LOCUS       DAJTNN010000001        89814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTNN010000001 DAJTNN010000000
VERSION     DAJTNN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 89814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.001"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..791
                     /locus_tag="OO150_000001"
     CDS             <1..791
                     /locus_tag="OO150_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5514292.1"
                     /translation="DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQT
                     GVCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGVWVDGMGSPSTGD
                     FHDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITY
                     DCGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQ
                     NGILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="OO150_000002"
     CDS             complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="OO150_000002"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5514293.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1758..3785
                     /gene="lpoA"
                     /locus_tag="OO150_000003"
     CDS             1758..3785
                     /gene="lpoA"
                     /locus_tag="OO150_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5514294.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQPVP
                     VSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEEL
                     LKSNTPLNVLVLNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSS
                     LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATTDSG
                     MTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAMLYAS
                     SRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDA
                     WSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3743..4138
                     /locus_tag="OO150_000004"
     CDS             3743..4138
                     /locus_tag="OO150_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5514295.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4158..4748
                     /gene="diaA"
                     /locus_tag="OO150_000005"
     CDS             4158..4748
                     /gene="diaA"
                     /locus_tag="OO150_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5514296.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4758..5333
                     /gene="yraP"
                     /locus_tag="OO150_000006"
     CDS             4758..5333
                     /gene="yraP"
                     /locus_tag="OO150_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5514297.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5453..6004)
                     /locus_tag="OO150_000007"
                     /pseudo
     CDS             complement(5453..6004)
                     /locus_tag="OO150_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            complement(6077..6712)
                     /locus_tag="OO150_000008"
     CDS             complement(6077..6712)
                     /locus_tag="OO150_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5514298.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6840..7358
                     /gene="yhbO"
                     /locus_tag="OO150_000009"
     CDS             6840..7358
                     /gene="yhbO"
                     /locus_tag="OO150_000009"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5514299.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7338..7781)
                     /locus_tag="OO150_000010"
     CDS             complement(7338..7781)
                     /locus_tag="OO150_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5514300.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     HRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7832..8134
                     /gene="yhbQ"
                     /locus_tag="OO150_000011"
     CDS             7832..8134
                     /gene="yhbQ"
                     /locus_tag="OO150_000011"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5514301.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8121..8624)
                     /locus_tag="OO150_000012"
     CDS             complement(8121..8624)
                     /locus_tag="OO150_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5514302.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8618..9142)
                     /locus_tag="OO150_000013"
     CDS             complement(8618..9142)
                     /locus_tag="OO150_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5514303.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADVSDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLHVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9351..10331
                     /locus_tag="OO150_000014"
     CDS             9351..10331
                     /locus_tag="OO150_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5514304.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10340..11218
                     /locus_tag="OO150_000015"
     CDS             10340..11218
                     /locus_tag="OO150_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5514305.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            11424..12431
                     /locus_tag="OO150_000016"
     CDS             11424..12431
                     /locus_tag="OO150_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR5514306.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12549..13793)
                     /gene="mtr"
                     /locus_tag="OO150_000017"
     CDS             complement(12549..13793)
                     /gene="mtr"
                     /locus_tag="OO150_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5514307.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13947..15836)
                     /gene="deaD"
                     /locus_tag="OO150_000018"
     CDS             complement(13947..15836)
                     /gene="deaD"
                     /locus_tag="OO150_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5514308.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15829..15909)
                     /gene="yrbN"
                     /locus_tag="OO150_000019"
     CDS             complement(15829..15909)
                     /gene="yrbN"
                     /locus_tag="OO150_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5514309.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16016..16900)
                     /gene="nlpI"
                     /locus_tag="OO150_000020"
     CDS             complement(16016..16900)
                     /gene="nlpI"
                     /locus_tag="OO150_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5514310.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDSDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17009..19144)
                     /gene="pnp"
                     /locus_tag="OO150_000021"
     CDS             complement(17009..19144)
                     /gene="pnp"
                     /locus_tag="OO150_000021"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5514311.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRSVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDICEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="OO150_000022"
     CDS             complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="OO150_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5514312.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19810..20754)
                     /gene="truB"
                     /locus_tag="OO150_000023"
     CDS             complement(19810..20754)
                     /gene="truB"
                     /locus_tag="OO150_000023"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5514313.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITIYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="OO150_000024"
     CDS             complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="OO150_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5514314.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21319..24039)
                     /gene="infB"
                     /locus_tag="OO150_000025"
     CDS             complement(21319..24039)
                     /gene="infB"
                     /locus_tag="OO150_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5514315.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREA
                     AEQAKREAAEKDKVSNQQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRV
                     AEEARRMAEENKWTDNAEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAA
                     RPKKGNKHAESKADREEARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITV
                     GELANKMAVKGSQVIKAMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEA
                     VMSDRDTGAAAEPRAPVVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVE
                     TENGMITFLDTPGHAAFTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVP
                     VVVAVNKIDKPEADPDRVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILL
                     QAEVLELKAVRKGMASGAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRA
                     MRNELGQEVLEAGPSIPVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKL
                     ARQQKSKLENMFANMTEGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSG
                     VGGITETDATLAAASNAILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAM
                     SGMLSPELKQQIIGLAEVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEG
                     ELESLRRFKDDVNEVRNGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24064..25551)
                     /gene="nusA"
                     /locus_tag="OO150_000026"
     CDS             complement(24064..25551)
                     /gene="nusA"
                     /locus_tag="OO150_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5514316.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(25579..26031)
                     /gene="rimP"
                     /locus_tag="OO150_000027"
     CDS             complement(25579..26031)
                     /gene="rimP"
                     /locus_tag="OO150_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5514317.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGHTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26238..26314)
                     /locus_tag="OO150_000028"
     tRNA            complement(26238..26314)
                     /locus_tag="OO150_000028"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26278..26280),aa:Met,seq:cat)
     gene            26662..28005
                     /gene="argG"
                     /locus_tag="OO150_000029"
     CDS             26662..28005
                     /gene="argG"
                     /locus_tag="OO150_000029"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5514318.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(28013..29638)
                     /locus_tag="OO150_000030"
     CDS             complement(28013..29638)
                     /locus_tag="OO150_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5514319.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFWAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQGNPPPEGQG"
     gene            complement(30097..30183)
                     /locus_tag="OO150_000031"
     tRNA            complement(30097..30183)
                     /locus_tag="OO150_000031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30147..30149),aa:Leu,seq:gag)
     gene            complement(30198..30530)
                     /gene="secG"
                     /locus_tag="OO150_000032"
     CDS             complement(30198..30530)
                     /gene="secG"
                     /locus_tag="OO150_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5514320.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30758..32095)
                     /gene="glmM"
                     /locus_tag="OO150_000033"
     CDS             complement(30758..32095)
                     /gene="glmM"
                     /locus_tag="OO150_000033"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5514321.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32088..32936)
                     /gene="folP"
                     /locus_tag="OO150_000034"
     CDS             complement(32088..32936)
                     /gene="folP"
                     /locus_tag="OO150_000034"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5514322.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(33026..34960)
                     /gene="ftsH"
                     /locus_tag="OO150_000035"
     CDS             complement(33026..34960)
                     /gene="ftsH"
                     /locus_tag="OO150_000035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5514323.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35060..35689)
                     /gene="rlmE"
                     /locus_tag="OO150_000036"
     CDS             complement(35060..35689)
                     /gene="rlmE"
                     /locus_tag="OO150_000036"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5514324.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35815..36108
                     /gene="yhbY"
                     /locus_tag="OO150_000037"
     CDS             35815..36108
                     /gene="yhbY"
                     /locus_tag="OO150_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5514325.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36265..36741)
                     /gene="greA"
                     /locus_tag="OO150_000038"
     CDS             complement(36265..36741)
                     /gene="greA"
                     /locus_tag="OO150_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5514326.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36989..38422
                     /gene="dacB"
                     /locus_tag="OO150_000039"
     CDS             36989..38422
                     /gene="dacB"
                     /locus_tag="OO150_000039"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5514327.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMIATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVIPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38462..39634)
                     /gene="cgtA"
                     /locus_tag="OO150_000040"
     CDS             complement(38462..39634)
                     /gene="cgtA"
                     /locus_tag="OO150_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5514328.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39650..40615)
                     /locus_tag="OO150_000041"
     CDS             complement(39650..40615)
                     /locus_tag="OO150_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5514329.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40742..40999)
                     /gene="rpmA"
                     /locus_tag="OO150_000042"
     CDS             complement(40742..40999)
                     /gene="rpmA"
                     /locus_tag="OO150_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5514330.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(41020..41331)
                     /gene="rplU"
                     /locus_tag="OO150_000043"
     CDS             complement(41020..41331)
                     /gene="rplU"
                     /locus_tag="OO150_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5514331.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41590..42561
                     /gene="ispB"
                     /locus_tag="OO150_000044"
     CDS             41590..42561
                     /gene="ispB"
                     /locus_tag="OO150_000044"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5514332.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42789..43067
                     /gene="sfsB"
                     /locus_tag="OO150_000045"
     CDS             42789..43067
                     /gene="sfsB"
                     /locus_tag="OO150_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5514333.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43115..44374)
                     /gene="murA"
                     /locus_tag="OO150_000046"
     CDS             complement(43115..44374)
                     /gene="murA"
                     /locus_tag="OO150_000046"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5514334.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(44429..44683)
                     /gene="ibaG"
                     /locus_tag="OO150_000047"
     CDS             complement(44429..44683)
                     /gene="ibaG"
                     /locus_tag="OO150_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5514335.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44843..45136)
                     /gene="mlaB"
                     /locus_tag="OO150_000048"
     CDS             complement(44843..45136)
                     /gene="mlaB"
                     /locus_tag="OO150_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5514336.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45136..45771)
                     /gene="mlaC"
                     /locus_tag="OO150_000049"
     CDS             complement(45136..45771)
                     /gene="mlaC"
                     /locus_tag="OO150_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5514337.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45790..46341)
                     /gene="mlaD"
                     /locus_tag="OO150_000050"
     CDS             complement(45790..46341)
                     /gene="mlaD"
                     /locus_tag="OO150_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5514338.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46346..47128)
                     /gene="mlaE"
                     /locus_tag="OO150_000051"
     CDS             complement(46346..47128)
                     /gene="mlaE"
                     /locus_tag="OO150_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5514339.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47136..47945)
                     /gene="mlaF"
                     /locus_tag="OO150_000052"
     CDS             complement(47136..47945)
                     /gene="mlaF"
                     /locus_tag="OO150_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5514340.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGCAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48155..49132
                     /locus_tag="OO150_000053"
     CDS             48155..49132
                     /locus_tag="OO150_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5514341.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49146..50132
                     /gene="kdsD"
                     /locus_tag="OO150_000054"
     CDS             49146..50132
                     /gene="kdsD"
                     /locus_tag="OO150_000054"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5514342.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50153..50719
                     /gene="kdsC"
                     /locus_tag="OO150_000055"
     CDS             50153..50719
                     /gene="kdsC"
                     /locus_tag="OO150_000055"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5514343.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSELLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50716..51291
                     /gene="lptC"
                     /locus_tag="OO150_000056"
     CDS             50716..51291
                     /gene="lptC"
                     /locus_tag="OO150_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5514344.1"
                     /translation="MSKARRWVTIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51260..51817
                     /gene="lptA"
                     /locus_tag="OO150_000057"
     CDS             51260..51817
                     /gene="lptA"
                     /locus_tag="OO150_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5514345.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51824..52549
                     /gene="lptB"
                     /locus_tag="OO150_000058"
     CDS             51824..52549
                     /gene="lptB"
                     /locus_tag="OO150_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5514346.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52597..54030
                     /gene="rpoN"
                     /locus_tag="OO150_000059"
     CDS             52597..54030
                     /gene="rpoN"
                     /locus_tag="OO150_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5514347.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTSETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54053..54340
                     /gene="hpf"
                     /locus_tag="OO150_000060"
     CDS             54053..54340
                     /gene="hpf"
                     /locus_tag="OO150_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5514348.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54458..54949
                     /gene="ptsN"
                     /locus_tag="OO150_000061"
     CDS             54458..54949
                     /gene="ptsN"
                     /locus_tag="OO150_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5514349.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54995..55849
                     /gene="rapZ"
                     /locus_tag="OO150_000062"
     CDS             54995..55849
                     /gene="rapZ"
                     /locus_tag="OO150_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5514350.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55846..56118
                     /gene="npr"
                     /locus_tag="OO150_000063"
     CDS             55846..56118
                     /gene="npr"
                     /locus_tag="OO150_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5514351.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56332..56964
                     /gene="yrbL"
                     /locus_tag="OO150_000064"
     CDS             56332..56964
                     /gene="yrbL"
                     /locus_tag="OO150_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5514352.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAYLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56961..57689)
                     /gene="mtgA"
                     /locus_tag="OO150_000065"
     CDS             complement(56961..57689)
                     /gene="mtgA"
                     /locus_tag="OO150_000065"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR5514353.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHRGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57686..58339)
                     /gene="elbB"
                     /locus_tag="OO150_000066"
     CDS             complement(57686..58339)
                     /gene="elbB"
                     /locus_tag="OO150_000066"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5514354.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58569..60905)
                     /gene="arcB"
                     /locus_tag="OO150_000067"
     CDS             complement(58569..60905)
                     /gene="arcB"
                     /locus_tag="OO150_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5514355.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(61001..61930)
                     /locus_tag="OO150_000068"
     CDS             complement(61001..61930)
                     /locus_tag="OO150_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5514356.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62605..67065
                     /gene="gltB"
                     /locus_tag="OO150_000069"
     CDS             62605..67065
                     /gene="gltB"
                     /locus_tag="OO150_000069"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5514357.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67078..68496
                     /gene="gltD"
                     /locus_tag="OO150_000070"
     CDS             67078..68496
                     /gene="gltD"
                     /locus_tag="OO150_000070"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5514358.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEQFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENKDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68680..69159
                     /locus_tag="OO150_000071"
     CDS             68680..69159
                     /locus_tag="OO150_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5514359.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            69176..69481
                     /locus_tag="OO150_000072"
     CDS             69176..69481
                     /locus_tag="OO150_000072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514360.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(69557..70021)
                     /locus_tag="OO150_000073"
     CDS             complement(69557..70021)
                     /locus_tag="OO150_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5514361.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPSEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(70018..70893)
                     /gene="nanK"
                     /locus_tag="OO150_000074"
     CDS             complement(70018..70893)
                     /gene="nanK"
                     /locus_tag="OO150_000074"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5514362.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(70890..71579)
                     /locus_tag="OO150_000075"
     CDS             complement(70890..71579)
                     /locus_tag="OO150_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5514363.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(71627..73117)
                     /gene="nanT"
                     /locus_tag="OO150_000076"
     CDS             complement(71627..73117)
                     /gene="nanT"
                     /locus_tag="OO150_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5514364.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVSGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(73226..74119)
                     /gene="nanA"
                     /locus_tag="OO150_000077"
     CDS             complement(73226..74119)
                     /gene="nanA"
                     /locus_tag="OO150_000077"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5514365.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(74241..75032)
                     /gene="nanR"
                     /locus_tag="OO150_000078"
     CDS             complement(74241..75032)
                     /gene="nanR"
                     /locus_tag="OO150_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5514366.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            75412..76778
                     /gene="dcuC"
                     /locus_tag="OO150_000079"
                     /pseudo
     CDS             75412..76778
                     /gene="dcuC"
                     /locus_tag="OO150_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(76821..77318)
                     /gene="sspB"
                     /locus_tag="OO150_000080"
     CDS             complement(76821..77318)
                     /gene="sspB"
                     /locus_tag="OO150_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5514367.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77324..77962)
                     /gene="sspA"
                     /locus_tag="OO150_000081"
     CDS             complement(77324..77962)
                     /gene="sspA"
                     /locus_tag="OO150_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5514368.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78357..78749)
                     /gene="rpsI"
                     /locus_tag="OO150_000082"
     CDS             complement(78357..78749)
                     /gene="rpsI"
                     /locus_tag="OO150_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5514369.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78765..79193)
                     /gene="rplM"
                     /locus_tag="OO150_000083"
     CDS             complement(78765..79193)
                     /gene="rplM"
                     /locus_tag="OO150_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5514370.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79411..80538)
                     /gene="zapE"
                     /locus_tag="OO150_000084"
     CDS             complement(79411..80538)
                     /gene="zapE"
                     /locus_tag="OO150_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5514371.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80732..81130
                     /locus_tag="OO150_000085"
     CDS             80732..81130
                     /locus_tag="OO150_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5514372.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81284..82651
                     /gene="degQ"
                     /locus_tag="OO150_000086"
     CDS             81284..82651
                     /gene="degQ"
                     /locus_tag="OO150_000086"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR5514373.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDCGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            82741..83808
                     /gene="degS"
                     /locus_tag="OO150_000087"
     CDS             82741..83808
                     /gene="degS"
                     /locus_tag="OO150_000087"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5514374.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(83871..84809)
                     /gene="mdh"
                     /locus_tag="OO150_000088"
     CDS             complement(83871..84809)
                     /gene="mdh"
                     /locus_tag="OO150_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5514375.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            85244..85714
                     /gene="argR"
                     /locus_tag="OO150_000089"
     CDS             85244..85714
                     /gene="argR"
                     /locus_tag="OO150_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5514376.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            86079..86342
                     /gene="yhcN"
                     /locus_tag="OO150_000090"
     CDS             86079..86342
                     /gene="yhcN"
                     /locus_tag="OO150_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5514377.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(86397..86669)
                     /locus_tag="OO150_000091"
     CDS             complement(86397..86669)
                     /locus_tag="OO150_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5514378.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(86761..88728)
                     /gene="aaeB"
                     /locus_tag="OO150_000092"
     CDS             complement(86761..88728)
                     /gene="aaeB"
                     /locus_tag="OO150_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5514379.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IIADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTERVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(88734..89666)
                     /gene="aaeA"
                     /locus_tag="OO150_000093"
                     /pseudo
     CDS             complement(88734..89666)
                     /gene="aaeA"
                     /locus_tag="OO150_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            complement(89674..>89814)
                     /locus_tag="OO150_000094"
     CDS             complement(89674..>89814)
                     /locus_tag="OO150_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5514380.1"
                     /translation="LLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLISRL
                     FV"
BASE COUNT        22069 a        24135 c        22315 g        21295 t
ORIGIN      
        1 atgacagcct ggatattaaa accacattac aggcaaacga cattccagga ctcacaaccg
       61 accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc gtatattctg
      121 ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc gttaatattg
      181 gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg atgaccattc
      241 cgaaaacgga tattgccgtc attaaaggcg tgtgggtcga tggaatggga agcccgtcta
      301 caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc gccccacagt
      361 cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc aatggtcaga
      421 agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt gatatcactt
      481 atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc gacggtacaa
      541 ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac aatgtgcccg
      601 atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct tttcagaacg
      661 gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc tggccagtta
      721 atctggtcgg tggtgagctg gaaacaggaa aatttcaggg cacagccacc attaccgtca
      781 tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca ctgttacccc
      841 tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat ttctgcgacc
      901 agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt gcgcagggca
      961 tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac catttcgcct
     1021 ttgcgacggt tttcatcttc ctttacccac gccagcagtt cgccaacggg cgcgccgtga
     1081 atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga ttcgcctaat
     1141 accgcaacga tatcttccag gctatctaac agacggtggg tggattcata aaaaatcagc
     1201 gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga tttggcaggt
     1261 aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa cgcagtgata
     1321 gcagcacacg gcccgggtag cggcaccacg cgaatccccg cttcacggca ggtacgcacc
     1381 agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag cgcaatgttt
     1441 tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc
     1501 agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt gtgacgagta
     1561 tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg ggtgatatcc
     1621 gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt atccgccgat
     1681 tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa
     1741 atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc
     1801 tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact cccgatcagt
     1861 ccactgctta tatgcagggc acagcgcagg ctgattctgc cttttatctt cagcagatgc
     1921 agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt gcactggtga
     1981 aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa gaactgaacg
     2041 attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg cagaaagatt
     2101 ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa caaaaccagc
     2161 aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc attgatttac
     2221 tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag cagaatattg
     2281 atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg ctggtgatca
     2341 acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg tttgataacc
     2401 gtaacgatcc cgatatgatg aaagccggga tcgccgactg gcagaaacgt tatccgaaca
     2461 atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct
     2521 cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta tttggtcgca
     2581 ctattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg gcggctcagg
     2641 tagctgccgc agacgtagca gaacaacctc agccgcaaac tgcggatggc gttgccagcc
     2701 cggcacaagc ctcggttagc gatctgaccg gtgatcagcc tgcggcccag ccggtgcctg
     2761 taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca tccgcagagc
     2821 tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag gttcagcagg
     2881 atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag ttgcttaaga
     2941 gcaacacccc gctgaacgtg ctggtactga accagccgga gaatatcgaa aaccgcgtca
     3001 atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg cgtcatattc
     3061 gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagttcattg ggcgatcgcg
     3121 tagccaatgc gtttgcgcag gagtggcaga aactgggcgg cggcaccgtt ctgcaacaaa
     3181 aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt atcgctttaa
     3241 cgggtacccc gattactccc agagcgacaa ccgactccgg catgacgacc aacaatccaa
     3301 cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt gtcgatgcgg
     3361 tgtacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc gccatgcgta
     3421 acggtagcca gagcggtgca atgctgtacg ccagctcccg cagcgcacaa ggcaccgcag
     3481 gcccggattt ccgtctggag atggaaggtt tgcagtacag cgaaatcccg atgctggcgg
     3541 gcggtaatct gccgttaatg cagcaggcac tcagcgcggt gaataacgat tattcactgg
     3601 ctcgcatgta tgcgatgggc gtcgatgcct ggtcgctggc aaatcatttc tcacaaatgc
     3661 gccaggttca gggttttgaa atcaacggta ataccggaag cctgacggca aacccggatt
     3721 gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta gtccccgcca
     3781 gttaaccacc aaacagaccg gcgatgcgtg ggaagtacaa gcgcgtcgct ggctggaagg
     3841 caaaggactg cgatttgtcg ccgctaacgt gaacgagcgt ggcggtgaga tcgatctgat
     3901 aatgcgtgaa ggctggacca ccgtttttgt cgaggtgcgt taccgccgct ctgcgcttta
     3961 tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac agactgcccg
     4021 cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt tcgatgtggt
     4081 agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc actcataatt
     4141 aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa agcattcaaa
     4201 ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc atgacgctgg
     4261 ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact tccgctgcca
     4321 atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg cccagcttac
     4381 ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac gatcgcttac
     4441 atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat gtattgttag
     4501 ccatttccac ccgtggcaac agccgcgata ttgttaaagc agttgaagcc gccgttacgc
     4561 gtgatatgac cattgtggca ttgaccggct atgacggcgg cgaacttgca ggtttgttag
     4621 ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt caggaaatgc
     4681 atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc cctcaccagg
     4741 atgattaagg agaatacatg aaggcattat cgccaatcgc agtccttatt tccgcgctgc
     4801 tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt accaaagccg
     4861 caactgaccc acgcagtgtc ggtacccagg tggacgatgg taccctggaa gtgcgcgtga
     4921 acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat gtcacggcgt
     4981 atcagggcaa agtgctgctg gttgggcagt caccaaatgc tgaactttcg gctcgcgcca
     5041 aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt cgtcagggcc
     5101 agccgattgg tctgggcgaa gcatctaacg atacgtggat caccaccaaa gtgcgttcgc
     5161 agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc gaaaacggtg
     5221 aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca gatattgcca
     5281 gccgggtgag cggcgtaaag cgggtaacca cagcgtttac gtttattaaa tagcagcccc
     5341 ttgtaatgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat gcaaaccgta
     5401 ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag aacaacagcg
     5461 ccagcccggc caccagacaa atcgccgcaa aaccccagcc gaggacaaac cccataaaca
     5521 ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccatcg
     5581 atacctgttg gcgacgcatc cccgccgcga ccggagccgc acagcaggta cacatcatgc
     5641 ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
     5701 gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca
     5761 ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc gcttgccacg
     5821 gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gctgcagtaa
     5881 acgctttgcc gtagtaatgt tgccatttca cataccagag accggcaatg acaacgagaa
     5941 agaaaagcgc gggtttccac cactgaatgg gtgttgccgc ctgagatgaa gactgaccag
     6001 tcatagcatt ccctaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
     6061 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca
     6121 cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat
     6181 caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca
     6241 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
     6301 agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
     6361 agtgggcatt ggcccccata gcactgacca ccagcatatg ctgcgcgccc agccgccgcc
     6421 cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc
     6481 ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt tcggtgacct
     6541 gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc atatcgccca
     6601 acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc aacatccgca
     6661 gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc
     6721 ctttacagaa ttgtctgcct tgcgctccac ggctcatgca ccagtcttaa tagaccatga
     6781 ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca
     6841 tgagtaagaa aattgccgtt ttaatcagtg atgaatttga ggattcagaa tttacttcac
     6901 ccgcagacga gttccgtaaa gccggacacg aagtaattac cattgaaaag caagcgggta
     6961 aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag
     7021 tgacgccagc ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc
     7081 gtggggacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc aaacctgttt
     7141 tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac
     7201 tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgcggaa ttttacgatc
     7261 aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac gatctgccag
     7321 cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca aatcattttt
     7381 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
     7441 ggggccgaca gcattctggc aaccggaaag cgatgattgt acgccttgcg cgcgaggtcg
     7501 ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg aattaacgct
     7561 accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc actcatctgc
     7621 gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaacaga
     7681 taaaaagcat tagcgcacca cagttcccct tcctgctgca cacaccaggt gacaacatgt
     7741 tgttttgcca accaacggct gatggtgatg agtgtttcca ttgctgttct cttttatact
     7801 gtgggcctga ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg
     7861 taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca
     7921 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt
     7981 ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg ttaagcaact
     8041 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag aactgttgag
     8101 cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccagaccg
     8161 gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgccc
     8221 ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg actgtacagc
     8281 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
     8341 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac cgccaacggt
     8401 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
     8461 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
     8521 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg cagcagggca
     8581 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
     8641 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
     8701 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
     8761 catgcagccc cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat
     8821 ctggatcttg tttacgcgcc gcaatcatca gcagatcgct gacgtcggca ctaaaactca
     8881 catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt
     8941 gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
     9001 catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
     9061 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
     9121 gggaacgaag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
     9181 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
     9241 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
     9301 cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc
     9361 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
     9421 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
     9481 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg
     9541 cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgcgcc gtggatatgg
     9601 cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gaatacgccg
     9661 ccgagcgtta tccgaatatt gagcgccacg tatcggtgca ggcttcggcg accaatgaag
     9721 aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt
     9781 tgtcgattca tcaggtgaaa cagctggcac gggtcacacc tgtaccactg gaagtgtttg
     9841 ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg
     9901 gtgagtcgcc taacaccgtg ggcgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa
     9961 cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg
    10021 aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc
    10081 acgcgctgga agaaccaacc agtctcaata ccttggaact gctgccggag ttaatggcgg
    10141 cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    10201 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa agcgcgtgga
    10261 tggagacgct cgggtcgatg tccgaaggca cgcagaccac ccttggcgcg tatcaccgta
    10321 aatggcagtg agaagagcaa tgaaatattc cttagggcca gtgctgtggt actggccaaa
    10381 agagacgctg gaagattttt atcagcaggc cgccaccagc agcgccgacg tgatttatct
    10441 tggagaagcg gtatgcagca agcgccgggc aaccaaagtt ggcgactggc tggagatggc
    10501 aaaatcgctc gccggaagtg gcaagcagat tgtgctctcc acgctggcgc tggtgcaggc
    10561 atcctctgaa ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc
    10621 cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca
    10681 cgcgctgaac tgctacaacg cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg
    10741 ctggtgtatg ccggtggagc tttcccgcga ctggctggtg aatctgctta atcagtgcga
    10801 tgagctgggc attcgcaacc agtttgaagt ggaagtcctg agctacggtc atctaccgct
    10861 ggcctactcc gctcgctgct ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga
    10921 aacctgctgc attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt
    10981 gtttgtactc aatggcattc agaccatgag cggctacgtt tacaacctcg gtaacgagct
    11041 gacatccatg cagggattgg ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt
    11101 cgcgatgctc gacgccttcc gcgctaatga aaatggcgct gcgccactat tgttgacggc
    11161 aaacagcgac tgtaacggct actggcggcg gctggcagga ctggagctgc aagcctaagt
    11221 aaatatgctc gtcatacttc aagttgcatg tgctgcatct gcattcgttc accccagtca
    11281 cttacttatg taagctcctg gggattcact ctcttgtcgc cttcctgcaa ctcgaattat
    11341 ttagagcaca gctcactttg ttaacaactt taactactct ttaatgcagt attaaagatt
    11401 aatcggtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg ttactcgatc
    11461 tggcccccat tcccgaaggt tcttcagcgc gagaagcatt ctcccactct ctcgatctcg
    11521 cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga
    11581 ctggcattgc cagtgctgcc acgtcggtat tgattggcta tctggcggcg aataccacca
    11641 cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg
    11701 aacagttcgg cacgctcaat acactctatc cggggcgaat cgatttgggg ctgggtcgtg
    11761 cgccaggtag tgaccaacgg acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg
    11821 ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc
    11881 cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca
    11941 gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact
    12001 tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagcaacttc aaaccgtcgg
    12061 cacgactgga aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc
    12121 gcgatgccga attcctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgcggcg
    12181 aaaccgggca actgccgcca ccgattcaaa atatggatca gttctggtcg ccgtccgaac
    12241 agtatggtgt gcagcaggcg ctgagtatgt cgttggtagg tgataaagcg aaagtgcgtc
    12301 atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtc aacgggcaga
    12361 ttttcgacca ccaggcgcgg ctgcattcgt ttgagctggc gatggatgtt aaggaagagt
    12421 tgttgggata gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg
    12481 tagacctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc
    12541 accgctgatt actgatacac cggcagtaaa ttaaagctcg ataaaatatg taccaatgcg
    12601 ttgccgacgc caaacaccag aatcagcgta atcatcggct tgccgcccca gacgcggaat
    12661 ttcgggctgc caaagcgttt acgcgatgcg cgggccagca gtgccggaac aattgccgcc
    12721 cagatggtag ccgctaaacc agcataacca atggcgtaca ggaatccatt cgggaacagc
    12781 agaccaccca caacaggcgg tgcaaaggtc aacaatgctg ttttcaagcg gcccatagcc
    12841 gagtcatcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg
    12901 aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaaggtccag gctacggctg
    12961 ttcagcacgc cgcttaatgc ctgtaccagc acatcaatat taccgccctt ctctgcaata
    13021 ccgataaact ccggacgcgg gatgttaccc atcgtcgcca gcaaccagat ggtatacagc
    13081 gccagtgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg
    13141 taatacttca tcaggcttgg cacgttaccg tgataaccaa acgacgccag gcagaacggc
    13201 aatgtcatca gcagatacgg cgcataagat gcattgcttt ctgcaacgtt gaacaatgtc
    13261 gcaggctgca catgccccaa caggctgcca aaggtgagga agaaggtaat gactttcgcc
    13321 cccagcacaa tcgccgtcat gcggctgacg gccttagtac tcaaccacac cacaaacgct
    13381 accagcagtg caaaaccaaa acccgccgcc cgtgccggga cgtttagcga catctctgcg
    13441 aaggtgtgat gcagaattga accactggca gaaatatagg cataggtcag gatatagagc
    13501 acaaaggcaa tggaaatacc gttgaccacg ttccagcctt tgcccagcaa atctttggtg
    13561 atggtgtcaa aactcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg
    13621 gaatgcagca tacagaacca ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc
    13681 ccggacatga ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc
    13741 accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct
    13801 tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacgcga
    13861 gtacaaaaga cagaaaaaaa gccccgatgg taaaaatcgg ggctgtatat attattttac
    13921 agattgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta
    13981 gagtcatcac gacgcggagc gcggccttca cgacgttcgc cgctaaaacg acgaccatca
    14041 ccacggccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgttcgcca
    14101 ccgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc atcgcccagt
    14161 aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg cagcacttca
    14221 cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttacca
    14281 atgtaacggc tgctgatgtc gccttcgtta gcaatcgcac caacgatatg acgaacttca
    14341 acaccatcat cacggcccac ttcaatgcgg tacagctgca tatcgccaac atcacgacgt
    14401 tcacgacgcg gacgatcttc acggtcacca cgcgggccac ggtcgttacg atcgcgcgga
    14461 ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggtacg
    14521 atcagagtac gttcaccctg tgccattttc agcagtgccg cagccagagt ttcgagatcc
    14581 agctcttcac cttctgcagt cggctgaatt ttgctcagca gtgcgcggta ttgatccaga
    14641 tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg gcgtttgccc
    14701 agcagttctg cgttcggcag ttctacttcc ggaatagtca gcttcatagt acgttcaatg
    14761 ttgcgcagca gacgacgctc gcggttctca acgaacagca gcgcgcggcc agcacgaccc
    14821 gcacgaccgg tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag
    14881 ttaactacca ggctgatacg ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc
    14941 aggatgtcca gacgaccatc tttcaggcgt tccagtgtct gttcacgcag cgcctggttc
    15001 atgtcaccgt tcagcgcggc gctgttgtag ccgttacgct caagagcttc agccacttcc
    15061 agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg
    15121 aaacgtacca gtgcttcgtt tttgcgcata ccccagacag tccagtagct ctggctgatg
    15181 tcaggacggg tagtcacgct ggactgaatg cgcacttcct gcggctcttt cataaagcgg
    15241 cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct
    15301 tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg
    15361 tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg
    15421 tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt
    15481 aattgcacgt cataacgctg gccgccgtac agagcaacca catttacgcc gcgcatgtgt
    15541 ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc
    15601 accagaatct gtggtgcttt cagctcagga tcaagattct gcaacagagg taaagagaat
    15661 gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga
    15721 tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct
    15781 tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag
    15841 tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc
    15901 aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc
    15961 tcacccctta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt
    16021 gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg
    16081 cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg
    16141 tggcgctgtc cgaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac
    16201 tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg
    16261 tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat
    16321 ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg agcttctgct
    16381 cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca
    16441 gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc ccgcgattca
    16501 agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag
    16561 catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaacact tcaggcatat
    16621 ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac
    16681 tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc
    16741 gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca
    16801 atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa
    16861 gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac
    16921 ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc
    16981 ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg
    17041 gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct
    17101 ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca
    17161 ctttctcaac gcgtttgtca gcgatttgag agatgtggac cagaccttct ttaccgccgc
    17221 cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccagtg tagacgcggc
    17281 ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc
    17341 cgtcggtcgc tgcgatcttc acagtaccgt catcttcgat ttcgatggta gtgccagttt
    17401 cttcggtcag ggcacggata acagagccgc ctttaccgat aacgtctttg atcttgtccg
    17461 ggttgatctt gatggtatgg atacgcggtg cgaactcaca gatatcgcca cgcggcgcgt
    17521 tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg
    17581 caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc aatgcagaga
    17641 taccctcgcg ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca
    17701 aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg
    17761 caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg
    17821 aagccataga agaggaaccg ttggattcag tgatttcaga cacaacacgt acggtgtacg
    17881 ggaatttgtc catatccggc atgactgcca gcacgctgcg cttcgccaga cgaccgtgac
    17941 caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga
    18001 agttgtagtg gaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg
    18061 cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg cgtttcacca cgggtgaaca
    18121 gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac atccagacca cggatcatat
    18181 ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacgtttt
    18241 tctcgatagc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa
    18301 gcagcgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt
    18361 cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca
    18421 gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg tttaccggct tctttcacca
    18481 gttcattgat gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca
    18541 gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagaaca gcggcttcag
    18601 taccggcaac aaccagatcc agtttgctct ctttcagctc gtcctgagtc gggttcagta
    18661 cgtactggtc attgatgtaa cctacgcgag cagcaccaat cggaccattg aacggaatac
    18721 cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg
    18781 ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca
    18841 gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttggacgac
    18901 cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt
    18961 taacggtcag tgggaagaag tcctgacctg gtttggcttt tttctggcca acaacggtaa
    19021 caaataccgc ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca
    19081 tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa
    19141 gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg
    19201 atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc
    19261 tctcattagc cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt
    19321 tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa
    19381 ctcggcagga attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg
    19441 tctttacgtt tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca
    19501 cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt
    19561 gcagtcagca gtgctacctg aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac
    19621 tcagaaacga ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata
    19681 tagaatgaaa ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt
    19741 aaacaattta cgcgacgtta agcggcagta ttctactcgt agcgacttgt tatcgcaaga
    19801 cggttaacat tacgccgggt attcaaccac caggcgacga ggcgcgacgc ggccttcatc
    19861 gtcaatttcg cccataccga taaacttgcc gttctcacct tctgtaacgc gaaccagtcc
    19921 ttccagcggc gcaccggatg tacgaactgg gttaccattt ttgaagtaaa cagaagacgt
    19981 taacggaaga ttcaccaccg ggtagtccga agctggactg tccattggca tcagtaatgg
    20041 atcaagtaac tccgcagccg gaatatcctg ctgttcggct tgttcgacca gttcacgcag
    20101 atgttccagg gtcaccatcc gttcaaccgg atatttactt accgccagac gacgcaggta
    20161 aataacatgc gcgccacaac cgagtttttc acccaggtca tcaatgatgg tgcggatata
    20221 agtgcctttt gagcagtgaa tttccagctc cagctcattg ccttcgtggc gaataaacag
    20281 caattcataa atggtaatcg ggcgcgcttc acgcggtact tcaatgccct gacgcgcata
    20341 ttcgtacagt tttttaccct gatatttcag tgctgaatac atcgaaggga tctgttcgat
    20401 atcgccacgg aaagtatcca gcgccgccgc cagttgctct gcgctaaagg ttaccggacg
    20461 ttcttcaacg atttgtccgt cggcatcaga agtatcggta cgctgtccaa gacgcgcaat
    20521 gacccgatag cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgaa
    20581 gcaaattggc aacatgccag ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt
    20641 ggcgttataa atccgtttca ctttttgcag cgcatcattg ctggacatac cctgcggttt
    20701 atccagcaac aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc
    20761 tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc
    20821 aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc
    20881 acgatacgca ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcttgc
    20941 aacgctttga tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat
    21001 actttggcat acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc
    21061 aggcgaggat ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc
    21121 gctacgcgct gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg
    21181 actgttagcc cctttttaaa attaatttca ggtggaaggg ctgttcacgt tggcctgata
    21241 agacgcgcca gcgtcacatc aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg
    21301 catggaaccc taaaaacctt aagcgatggt acgttggatc tcgatgattt cgaatacttc
    21361 gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg ccgataccac attccatacc
    21421 gttacggact tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta
    21481 gataaccacg ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt
    21541 aaccatacag cctgcgatgg caccaaattt cggcgatttg aacacgtcac gaacttccgc
    21601 cagaccgata atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac
    21661 ttcgtcaatc aggttataga tgacggagta gtaacgcaga tctaggcttt ccgcttcaat
    21721 cactttacgt gcagaggcat cagcacgtac gttgaagcca accaggatgg cgttggacgc
    21781 cgcagccagg gtggcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac
    21841 tttaacttcg tcagtagaca gtttcagcaa ggagtcggag atcgcttcca cagaaccctg
    21901 tacgtctgcc ttcaggacga tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa
    21961 catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg
    22021 acgatacagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc
    22081 cgcagccggt acgccggaca ggccgaggat ttccaccgga atggacggac ccgcttccag
    22141 cacttcctga cccagttcgt tacgcatcgc acgaacacga ccgtattcga agccacacag
    22201 aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg
    22261 acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt
    22321 cagctccaga acttccgcct gcagcaggat agcgtccagc agttcatcga tgccggtacc
    22381 cgctttcgca gatacgtgta cgaactggct ttcaccgccc cactcttctg gcaggatgcc
    22441 atactgggag agttcgttct taacacgatc cggatcggct tctggtttat caatcttgtt
    22501 cactgcaacc accaccggta cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg
    22561 cggcatcacg ccgtcgtcgg cagcaacaac caggactacg atgtccgttg cctgcgcacc
    22621 acgagcacgc attgaagtaa acgcggcgtg ccctggggtg tccaggaagg tgatcatgcc
    22681 gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga
    22741 ggccactttc gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc
    22801 catgatggtc acaaccggcg cgcgcggttc agccgcagcc cccgtgtcac ggtcgctcat
    22861 taccgcctct tccagctcgt tttcacgacg caggataact ttgtggccca tctcttcagc
    22921 aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt
    22981 catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac
    23041 ggtgatggtt tcgccgatca caacgtcacg gttaacggcc tgagctggct tctggaagcc
    23101 ttgctgcagg gaagaacctt tacgttttcc gcctttacct ccacgtactg ctgcgcgagc
    23161 ttcttcacga tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt
    23221 cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg
    23281 gcgagcatgt tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt
    23341 atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc
    23401 ttcttcgagt ttacgacgcg cttcttcttc agctttacgc ttgagctctg cagcttcctg
    23461 ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac
    23521 tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgtt tcgcttgttc
    23581 agcagcttca cgcttagctt gttctgcggc ctcacgttca gctttttgtt gcgcctcgcg
    23641 tttagccgat tcttctgcct cacgacgggc ttgctcttcc gcttcacgct gcgcttgctc
    23701 ttccgcttca cgctgcgctt gctcttccgc tgcaaggcgt tcagcctctt gcggatcgcg
    23761 tttcacaaag gtgcgtttct tgcggacttc gatttgtacc gatttgcttt ttccaccggt
    23821 accaggaatg ttaagggtgc tgcgtgtttt acgttgcagc gtcaatttgt ccgggcctga
    23881 atttttctga ttcaggtggt caatcaaagt ctgtttctct tgtgcggaca cagagtcgtc
    23941 agcagacttc cggatacctg catcagcaaa ttgctgtacc aggcgttcca cggaggtctg
    24001 tcgctctgcg gccagcgttt taatcgttac atctgtcatg ctgttccttc ctgctacagt
    24061 ttattacgct tcgtcaccga accagcaaat attacgggca gccataatca gtgctccggc
    24121 tttttcgtcg gtcacccctt cgatatcagc cagatcatca atgccctgtt cggcgagatc
    24181 ttccagcgta caaacgccac gggcggccag tttgaatgcc aaatcacgat ctatcccttc
    24241 aaggttcagc agatcgtcag ccggtttgtt atcaccgagg ctttcttcct gggcctgtgc
    24301 aatggtggcc agtgcatttt tagcacgctc gcgcagtgct tcaacggtcg gctcatcaag
    24361 gccttcgatt tccaacagct ctttcatcgg cacataggcc agttcttcca gcgtcgagaa
    24421 gccttcttct accagaacag tcgcgaagtc ttcgtcgatg tcgagatatt tggtgaaggt
    24481 gtcgatcgct gcgtgcgctt ccgcctgatg cttagcctgc aggtcgtcaa cggtcatcac
    24541 gttgagttcc caaccgctca gctgcgaagc cagacgcacg ttctgaccgt tacggccaat
    24601 cgcctgcgcc aggttaccgg cttcaacggc gatatccatg gtgtgtttat cttcatccac
    24661 cacgatagaa gcaacgtctg ccggtgccat tgcgttaatc acaaactgcg ccgggttatc
    24721 atcccacagg acgatatcga tacgctcgcc gcccagttca gtagaaaccg cctgaacacg
    24781 cgcgccacgc atacctacac aggcacctac cggatcgata cgtttatcgt tggttttcac
    24841 cgcgattttc gcacgagaac ccggatcgcg agccgctgct ttaatttcaa tcacttcttc
    24901 gccgatttct ggcacttcaa tacggaacag ttcgatcagc atttccggct tggaacgagt
    24961 gacgaacagt tgcgcgccac gcgcttccgg gcgaacggaa tagagcacgc cacgaacgcg
    25021 gtcgccaggg cggaagtttt cacgcggcag catatcttcg cgcaggatca cggcttcagc
    25081 attgttgccc agctccagag agatgttgtc gcggtttact tttttcacca cgccggtgat
    25141 gatttcacct tcgtgttcac ggaactgatc aaccaccatc gcacgttcgg cttcacgcac
    25201 tttctgcacg ataacctgtt ttgccgtctg ggtagtgata cggtcaaagg taacagactc
    25261 aatctgatct tcaacgtaat cgcccaggtt caggctttca tcttcataac gtgcggcttc
    25321 aagggtgatt tccttggtcg gctgggtgac ttcatcaaca actaaccagc gacggaaggt
    25381 gtcaaaatca ccgcttttgc gatcgatctg tacgcggacg tcgatctctt gttcatattt
    25441 tttctttgtt gctgtcgcca gcgcgctttc caatgcttcg aaaatcttct cgcgaggtag
    25501 cgccttttca ttggatacgg cttcaactac agccaaaatt tctttgttca tcgcgggctt
    25561 ttcacctcat ccagactatt aaaagtgggg aaccaggttc gccttctgga tattactcag
    25621 cgcgaacact tcatctttac cttcgacggt aactgtgatc atttcaccgt ctaccgcttt
    25681 gataacgccc tgccatttac gacggttttg taccgccata cggagaacca gagtcacctc
    25741 ttctccgaca aaacgggcgt agtgttcagc cgtgaacagt gggcgatcga gacccggtga
    25801 ggagacttcc aggttataag caacggtgat gggatcttca acatccagca cagcacttac
    25861 ctggtggctc acatcagcac aatcatcaac attgatgcca tcttcactat caatatagat
    25921 gcgcagtgtg gatgtgtgac cgcgaataaa ttcgatgcca accagttcaa aacccagggc
    25981 ctcaactggc gcagtaatca tctctgttaa tttttgctct aatgtggaca agcccacccc
    26041 caagacataa aaaaagggcc taaagcccag ttattctgta gtcagataac aaaaaacccc
    26101 gataaatcgg ggctttatat aactgaaccc tataaccgca actgcggtct ggagcacttt
    26161 ccagaaggat tttttcaaat cccactacga aggccgaagt cttcacagta tatttgaaaa
    26221 aggactctaa gggaaagtgg ttgcgggggc cggatttgaa ccgacgacct tcgggttatg
    26281 agcccgacga gctaccaggc tgctccaccc cgcgcctgaa actcggcaaa ttctactcgt
    26341 tttgggtaaa agatgcaaat actgctggga tttggtgtac cgagacggga cgtaaaatct
    26401 gcaggcatta tagtgatcca tgccacattt tgtcaacgtt tattgctaat cacgtgaatg
    26461 aatatccagt tcactttcat ttgttgaata cttttgcctt ctcctgctct cccttaagcg
    26521 cattatttta caaaaaacac actaaactct tcctgtctcc gataaaagat gattaaatga
    26581 aaactcattt attttgcata aaaattcagt gagagcggaa atccaggctc atcatcagtt
    26641 aattaagcag ggtgttattt tatgacgacg attctcaagc atctcccggt aggtcaacgt
    26701 attggtatcg ctttttctgg cggtctggac accagtgccg cactgctgtg gatgcgacaa
    26761 aagggagcgg ttccttatgc atatactgca aacctaggcc agccagacga agaggattat
    26821 gatgcgatcc ctcgtcgtgc catggaatac ggcgcggaga acgcacgtct gatcgactgc
    26881 cgcaaacaac tggtggccga aggtattgcc gctattcagt gtggcgcatt tcataacacc
    26941 accggcggcc tgacctattt caacacgacg ccgctgggcc gcgccgtgac tggtaccatg
    27001 ctggttgctg cgatgaaaga agatggcgtg aatatctggg gtgacggtag cacctataaa
    27061 ggaaacgata tcgaacgttt ctatcgttat ggtctgctga ccaatgctga actgcagatt
    27121 tacaaaccgt ggcttgatac tgactttatt gatgaactgg gcggccgtca tgagatgtct
    27181 gaatttatga ttgcctgcgg tttcgactac aaaatgtctg tcgaaaaagc ctactccaca
    27241 gactccaaca tgcttggtgc aacgcatgaa gcgaaggatc tggaatacct caactccagc
    27301 gtcaaaatcg tcaacccgat tatgggcgtg aaattctggg atgagagcgt gaagatcccg
    27361 gcagaagaag tcacagtacg ctttgagcaa ggtcatccgg tggcgctgaa cggtaaaacc
    27421 tttagcaacg acgtagaaat gatgctggaa gctaaccgca tcggcggtcg tcacggcctg
    27481 ggcatgagcg accagattga gaaccgtatc atcgaagcga aaagccgtgg tatttacgaa
    27541 gctccgggga tggcactgct gcacattgcg tatgaacgtc tgttgaccgg tattcacaac
    27601 gaagacacca ttgagcagta tcacgcgcat ggtcgtcagt tgggccgtct actgtaccag
    27661 gggcgttggt ttgactccca ggcgctgatg ctgcgtgact ctctgcaacg ctgggttgcc
    27721 agccagatca ctggtgaagt taccctggag ctgcgccgtg ggaacgatta ttcaatcctg
    27781 aataccgtct cagagaacct gacctacaag ccagagcgtc tgacgatgga aaaaggcgac
    27841 tcggtgttct cgccagatga tcgtattggt caattgacca tgcgtaatct ggatatcact
    27901 gatacccgag agaaactttt tggttatgcc aaaactggcc tgctctcctc ctctgccact
    27961 tcaggcgtgc cgcaggtgga gaatatggaa aacaaaggcc agtaattcga catcaaccct
    28021 gcccttcagg gggggggtta ccttgcttat cctctgtaaa ctcctttctg aacatcacat
    28081 aatcgaaaac atcatggttt gcatccacaa cccgtgagtc tcctttatag tgtgcaatca
    28141 cttcctcaag cgtttgcggc tgttccggct ttgttacccc catccacgca ttaataaggt
    28201 aattattgta agctgtcgaa aagatgttgt tttccgttgt gcgatccacg ccatccccaa
    28261 gaacggggct ataccagata aacatcggga catgatatgc ctgctggcta gcctccctgc
    28321 ctccatgaaa atagacgttc ttcttcgtag ggtcacgttc caggccgtgg tcggcaaaat
    28381 acatgaccga ggcgcggcga tcttttaata attcaaaaac ctgacctagc aaactatctg
    28441 tgtaatgaat ggagttgtca tagcaggcat cctgatcgtc ctgcggttga aacacggcgc
    28501 tggattgcgg ataggcgcta caagccggtt catggcttcc atttaaatga agaacaatca
    28561 gtttcttttg ctgcgtattt tgctgtaatg cttgcgataa atgcggcaac aacaattcat
    28621 caaatcctct gacataaact gtttccatgg cgcgcatggc gatactggta actgcagtac
    28681 cattctgccg aaaagcggat tgcgagctta gccagaaagt ctgaaatcct gcttgattag
    28741 ccatattaat aatgttgtcg gggtaattat gaatgtcatg actcaaaaca gaatcagcag
    28801 ttaaagaaag gggaaccgac agcgcagtgt aaggtgcgcc gcttattgct tgattaaaca
    28861 gtttgatctg ttttctttgt gcttcaactt gcggtgtcgt agagcgtgta tatccataca
    28921 aagacatatt gtcgacacgt acagactccc ccacaatcaa cacgtaggta tcaatacctg
    28981 tatccctgac tgataattga aaatacggca ccgtgtttgc aattgagagt aatctttgat
    29041 gctctttcgc tgctaaagca aaatagttga gattgaaaaa cggcgtatag gtagcaaatc
    29101 gcgacgctag tatatatgga ctgaacgttt ttttattttt tgcatcttta taagcaaatt
    29161 gacaagcgga aaataaactg cccgaaatga caatcagcaa taatattcct gtcacttttt
    29221 ttgtcggcaa ggaaacatca tattttatta ttactgccca aaaaagaagg gataaaaaag
    29281 caaaggcaca tagataaggg atatacatcc ccagcatttt gacaacttca tccggatcac
    29341 tctggagcac actaatcgca aatccatcat tgaaagttgt accaaaggta caccatgaat
    29401 agaggctaat actcatgtct gccgcagtca gtaacacaaa tggtgcggca acaagcagac
    29461 gaagcggaag ggatgatata cgttttacaa ccaggatggt caggacgaag aacagcaatc
    29521 gctgaaccat atgcgcgccg gaagaggcga ctaagttcgt aataccaaaa acaataacac
    29581 aaagatacaa caaccaatga gatttaaaac ttctagcaaa tttgttgaat actgtcatcc
    29641 agaaatccaa gttcacaacc aatataaaca attatcaaca atgttcaatt ttatatcaga
    29701 catgtttttc aactctgatc catatcagac cgaaacactg ttagtttttt ctttttaaat
    29761 gatgcggcca ttgcgttctc agtaattaaa taacattatc aatgcgtctc taaacattac
    29821 gcagaacgta gccatcagta gttatactga caggagcagg atggtttatc gagaagtaaa
    29881 catatccgta ttcgtaccgg aactggagtt gacacaataa agtgccaatt atgtcagcag
    29941 aagggaaaag tttataacta aggcgtaccg gcaccatcgt ttcaaggtac cagctacgag
    30001 taaagcaact ggacgagata cagatacctg acaaccattc ctcagaccag gaccaaaacg
    30061 aaaaaagacg cttttcagcg tctcttttct ggagtttggt accgaggacg ggacttgaac
    30121 ccgtaagccc tattgggcac taccacctca aggtagcgtg tctaccaatt ccaccacctc
    30181 ggcacggata ctacttttta gttcgggata tcgctggtcg gcttagccgg agcagctggt
    30241 tgagtttgtt cggttttcgc cggtgcactc agattttccc attcgctacc tttattggtt
    30301 ttgttgctgt tgatgttacc cagcaccaga ctgatgatga agaataacgt tgccagcagc
    30361 gccgtcatgc gggtcatgaa gttaccagaa ccacttgaac caaacagcgt agcggaagcg
    30421 cctgctccga aggaggctcc catatcagcg cctttacctt gctgcagcat gatcagacca
    30481 acaaggccaa ttgccacaat aaggaaaact accaaaagag cttcgtacat aatcaacctg
    30541 ttccttgcgg agttgccgcg taccaatgct tcaaccaata aagcgggagt ttttaatctt
    30601 tcccactgaa gcgggtgtga atactaacca aagcgaatga ccttcgcaag ggcaatttta
    30661 tcgcattgta tcaactgcgg aaaaaaacag caaaaccgat gtattcgctg agaaaaaagg
    30721 cgacaatcgc cgccttttta gccagtcatt tatcgtttta aacggctttt actgcatcgg
    30781 cgatgcggtg tgcaaattca gtcacctgcg cttcgtcttc gccttccacc atcacgcgaa
    30841 ttaacggttc ggtgccggat ttacgcagca acacgcgtcc acggttgccc agcgcagctt
    30901 caacctctgc ggtcacggct ttaactgact catgctcaag cggatcgccg ctacctgcgg
    30961 tgtaacgcac gttaaccaga atctgcggga acattttcat gccgctgcaa aggtcgtgca
    31021 ggctcatatg gttacgtgcc atcgccgcca gcacctgcaa gccagcaacg atgccgtcac
    31081 cggtagtggt tttatccagc aggatcacat gaccggaatt ctctgcaccg atacgccagc
    31141 ctttctcctg cattttttcc agtacgtagc ggtcacccac tttcgcgcgc gcaaatggaa
    31201 tacctaactg tttcagcgcc agctcaagcc ccatgttact catcaatgta cccacagcac
    31261 caccacgcag ctggccctga cgaagacctt cacgcgcgat gatatacatg atctgatcac
    31321 catcgacttt attgccttca tggtcaacca taatcacgcg atcgccgtcg ccgtcgaagg
    31381 caataccgag atccgctttt tcagccagca cacgagcctg gagcgcgcga acgtcggtag
    31441 cccccacttc ggcattgatg tttacaccgt tcggctcaca accgatagcg ataacgttcg
    31501 cccccagttc acgcagcacg ttcggcgcga tgtgataagt cgcaccgttt gcgcaatcca
    31561 ccacaatctt cagttcgctg aggctaagtt cgttcgggaa cgtggctttg caaaactcga
    31621 tatagcgacc cgcagcatca acgatacggc tggctttacc cagttctgcc gaatcaacac
    31681 agctgatctc tttttccatt tccgcttcga tggcctcttc taccgcatcc ggtagtttgg
    31741 tgccgtcgat agagaaaaat ttaatgccat tatcgtagaa cgggttatgc gatgcagata
    31801 tcacaattcc ggcctctgcg cggaaggtac gcgtcagata agccacggcc ggtgttggca
    31861 tcgggccagt gaagagtgcg gaaaggcccg ctgccgccag acccgcttcc agtgctgact
    31921 ccagcatata gccagaaata cgcgtgtctt taccaataat gatcttacgg gagccgtggc
    31981 gcgccagcac tttacccgcg gcccaaccca gcttaagcac aaaatcaggt gtgatcggcg
    32041 catcccctac acgaccacga atcccatcgg taccgaaata tttacgatta ctcatagcgt
    32101 ttgttttcct ttgcagacag agtggcttcc accacccgca tcgcttctac ggtttctttg
    32161 acgtcatgaa cacgaataat gtgcgcgcct tgcattgcgg caatgaccgc acaggccaga
    32221 ctaccgctca ggcgctcgga cggccccacg ttcagcagct gcccaatcat cgattttcgt
    32281 gacataccta ccaacagcgg caggttgaaa tggtgaaatt cagccaggcg cgccagtaat
    32341 gaatagttat gggagagatt tttaccgaaa ccgaatccgg ggtcgagcaa caatttctct
    32401 tttgcgatac ccgcctgctc gcaacgtgct atttgctcaa taaagtagcg attcacttct
    32461 gcaaagacat cgtcatactt cggagcttcc tgcatggttt ttggatttcc ctgcatatgc
    32521 atcagacaaa ccggtaaacc ggtttctgcc gccgcctcca gagcgccagg ttcggaaagg
    32581 gagcggatat cgttaataat gtgagcgcca actttcgctg actcacggat gacttctggt
    32641 ttggatgtat ctaccgagag ccagacttcg aagcgttgag caattgcctc aaccacagga
    32701 ataacacgtt gcaactcttc ttcaacgcta acttccgccg cccctgggcg cgtggactcg
    32761 ccaccaacgt caatgatcgt cgcgccagcg ttgatcatca gattcgcatg tttcaccgca
    32821 tctatcagcg agttatgcgt gccaccatcc gaaaaggaat caggcgtgac gttgaggatc
    32881 cccattacgt gaggatggct aaggtccagt gaagtgccct gggcaaagag tttcatggtg
    32941 ttatccctgg tatgaattga taagaaaaaa ccccggagca cgccccgggg ttttcggtac
    33001 aaatacagtc atctgatgcg ggaacttact tgtcgcctaa ctgctctgac atggtgttac
    33061 ccgggttcgg cgtacgcggt tcatcaaccg gacgaggagc ctttggacta ccattgtcgc
    33121 cagaattgtt agaagcgcct ggttcttccc agcccgctgg cggacgtaca tcgcgacgtg
    33181 ccatcaggtc atcaatctgc ggtgcgtcga tagtctcata tttcatgaga gcatctttca
    33241 tcgcatgcag aatatccata ttgtcggtca gaagctgacg cgcacgatta tagttacgct
    33301 caatcagtgc tttcacttcc tggtcgatga tacgtgcagt ttcatcggac atatgtttcg
    33361 ctttcgctac gctacggccg aggaacactt caccttcttc ttccgcgtac agcagtggac
    33421 ccaatttctc agagaagccc cactgagtca ccatattacg cgccaggttg gtcgcaactt
    33481 taatatcgtt ggacgcaccg gtagaaacat gttccggccc gtagatgatc tcttctgcca
    33541 gacgaccacc gtacagcgta gaaatctggc tttccagttt ctgacggctg gcgctgattg
    33601 cgtcgccctc aggcaagaag aaagtcacac ccagcgcacg accgcgtggg ataatcgtca
    33661 ctttgtgcac cggatcgtgt tccggcacca agcgaccgat aatcgcatga cccgcttcgt
    33721 ggtaagccgt cgattctttc tgcgcttccg tcatcaccat ggagcgacgt tccgcaccca
    33781 tcatgatttt atctttcgct ttttcgaact caatcatcga cacaacgcgt ttgttgccac
    33841 gagcagcgaa cagtgccgct tcgtttacca ggttcgccag gtcagcaccg gagaaaccag
    33901 gagtaccacg ggcaatgatt gccgcgtcga tatcgggtgc caatggtacg cgacgcatgt
    33961 gaactttcag gatctgctca cgaccgcgaa catctggcaa gccaaccaca acctgacggt
    34021 cgaaacggcc aggacgcagc agggctgggt caagaacgtc cggacggtta gtcgcggcga
    34081 taacgatgat accttcgtta ccttcgaagc catccatctc aaccagcatc tggttcagag
    34141 tctgttcacg ttcatcgtga ccaccgccca gaccagcgcc acgctggcgg cctacggcgt
    34201 cgatttcatc gataaagatg atgcacggtg ccgctttctt cgcctgttcg aacatgtcac
    34261 gaacacggga tgcacccaca ccgacgaaca tttctacgaa gtcagaaccg gagatagtaa
    34321 agaacggaac tttcgcttcg cctgcaatcg ctttcgccag caacgtttta ccggtacccg
    34381 gaggaccgac catcaggacg cctttcggga tcttaccgcc gagtttctgg aagcggctcg
    34441 gctcgcgcag atactctacc agttcagcaa cttcttcttt tgcttcgtcg cagcccgcaa
    34501 cgtcagcaaa ggtcgttttg atctgatctt ccgtcagcat gcgcgctttg ctcttaccaa
    34561 acgacatggc acctttgcca ccgccgccct gcatttgacg catgaagaag atccagacac
    34621 caatcagcaa cagcatcggg aaccaagaga tgaagataga agccagcagg cttggttctt
    34681 caggcggttc accgacaacc ttgacgttct tggtcaacag gttatccagt aatttcggat
    34741 cctgaaccgg aatgtaagtg gtataacggt tactatcttt cttggtaacg ttgatttcac
    34801 gtccgttgat acgcgcttca cgaacctggt cgttattgac ctcttgtagg aaggtagagt
    34861 aatccacctt acggccatta gactcgctgg gcccaaagct ctggaatact gacatcagca
    34921 caacggcaat gaccagccag agtattaggt ttttcgccat gtcactcaag ggattaacct
    34981 cttattacaa ctgtgttaaa aacagcgtca ggatactcta tatccagcat ctttcaaact
    35041 ttcgtctgaa atctcccggt tagggtttac gcccggtcgc tacaatatac acttcccgcg
    35101 aacgtgcacg agaagagtcc ggcttacgaa ctttgacctt cgtaaacagg gagcgaattt
    35161 cccttagata ctcatcgaaa ccttcgccct ggaacacctt cactacaaaa ctgccacctg
    35221 gcgctaatac atcacgacac atttctaacg ccagttccac cagatacatg gcacggggga
    35281 tatccaccgc cggtgttccg ctcatgtttg gtgccatatc ggacatgaca acctggactt
    35341 tgctgtcgcc aacgcgctcc agcagtgctt tcatcaccag ttcatcacga aaatcgccct
    35401 gaagaaagtc cacaccaacg ataggatcca taggtaaaag atcgcaagcg atgatgcggc
    35461 ctttgccgcc aatttgggtg accacatatt gtgaccaacc acccggagca gcaccaaggt
    35521 cgacaaccgt cattcccggt ttaaagagtt tgtcactttg ctgtatttca tcaagtttaa
    35581 accaggcacg ggaacgtaac ccctttttct gtgcctgttg aacatattta tcgctaaagt
    35641 gttcctgaag ccagcggctg gagctggcag aacgcttctt acctgtcatt taactttccc
    35701 atggggataa ctcatcgtaa ccaattgcgt aaatttttac gcgcctattt ggtgatatat
    35761 gggagatggc ggtagaatga cccgttttca atcccaacgt aagcaaaaaa tacgatgaat
    35821 ctgagtacta aacaaaaaca gcacctgaaa ggtctggcac atccgctcaa gccagttgtt
    35881 ctgcttggca gtaatggttt gaccgaaggg gtgctggccg agattgaaca agcgttagag
    35941 caccatgaac tcatcaaggt gaaaatcgcc accgaagatc gcgaaacaaa aaccttgatc
    36001 gtggaagcta tcgtgcgcga aaccggcgcc tgtaatgtac aggtcatcgg taaaacgctg
    36061 gtgctttatc gcccaactaa agaacgtaaa atctcgctgc cacgctaaga ttatcctaaa
    36121 gttacacaca tttgctgtgt aaaacgaggg gttttccgca ggcaggagag caaaatgcca
    36181 cgctctgttc gttgataaaa ggccgcatag cggccttttt cctttcttta caatacatca
    36241 acatcttgat gtattgggta attcttacag gtattccacc ttaattactt caaattctac
    36301 ttcgccgccc ggcgttttga tgaccacaac atcatcttct tctttgccga tcaggccacg
    36361 agcaataggc gagtttacag aaatcaggtt ttgtttaaag tcagcttcgt catcgccaac
    36421 gatgcgataa gtctgttctt cgtcagaatc cagattcagc accgttacgg tagcaccaaa
    36481 aataacgcgc ccattgttgg gcattttggt gacatcaatc acctgcgcgt tcgacagctt
    36541 ggcttcgatg tctttaatac ggccttcgca gaaaccctgc tgttcacgag ctgcgtggta
    36601 ttcggcgttt tctttcaggt cgccatgctc acgcgcttcc gcgatagcag caatgatttc
    36661 aggacggcgc acagatttca gaaaatccag ctcttcgcgt aatttttcag cgccgcgtaa
    36721 ggtcatcgga atagcttgca tttgttatac ctcttgaata ttcctgatgg ggcaagtctt
    36781 cacccacccc ggctgttcgg cctgcccggc ataactcctc accgggacca gaagcaaaaa
    36841 aataccgacc cgggtacaag tcccaggtca gctacaattc acattttgat agtcatttta
    36901 ccctgaagtt cccgaagggt catcgtttac tttatagggc gttgcgccgt agtatgacgg
    36961 ctcgattcca ggttgttagc gcgagattat gcgattttcc agatttatca tcggattgac
    37021 cagctgtata gcgttcagtg ttcaggccgc gaatgttgat gagtacgtta ctcaactccc
    37081 cgctggtgcc aaccttgccc tgatggtgca aaaagtcggc gcgtcggccc ccgctattga
    37141 ttaccacagt cagcagatgg cgctgcccgc cagtacgcag aaagtgatta ctgcgctggc
    37201 ggcgttgatt caactcggcc ccgattttcg tttcaccacg acgcttgaaa ccaaaggcaa
    37261 tgtggaaaac ggcgtactta agggtgactt agtggcgcga tttggtgccg atccgacgtt
    37321 aaaacgtcag gatattcgca atatgatcgc gactttgaaa aaatctggcg tcaaccaaat
    37381 cgatggcaat gtgttgatag atacctccat tttcgccagc cacgataaag cccccggctg
    37441 gccatggaat gacatgacac aatgctttag cgctccgcct gccgctgcca tagttgaccg
    37501 caactgtttc tccgtctcgc tctacagtgc cccaaaacct ggtgatatgg cttttatacg
    37561 cgtggcatct tattaccccg ttacgatgtt cagccaggta cgcaccctcc cccgaggttc
    37621 tgccgaagcg caatactgcg aactggatgt cataccaggc gacctgaacc gctttacgct
    37681 gacgggatgc ctgccacaac gttctgagcc gctcccgttg gcttttgccg tgcaggatgg
    37741 agccagctat gccggggcaa ttctgaaaga tgagttaaaa caggcaggta tcacctggag
    37801 cggaacactg ctgcgccaga ctcaggttaa cgaacctgga acggtagttg ccagtaaaca
    37861 gtcggccccg ttgcacgatc tgcttaagat tatgctgaaa aagtcggaca acatgatcgc
    37921 cgatacggtt ttccgcatga taggccatgc gcgcttcaat gtgcctggaa catggcgggc
    37981 cgggtcggac gccgtgcgtc agatcctgcg ccagcaagcc ggtgtcgata ttggaaacac
    38041 cattattgcc gatggttcag ggctttcgcg gcataacctg attgcccccg ccaccatgat
    38101 gcaggtgttg caatacattg cccaacacga caatgaactt aactttatct ccatgctgcc
    38161 gctggcgggc tatgatggct ctttgcagta ccgtgcaggt ctgcatcagg cgggcgtgga
    38221 tggaaaagtc tcagcgaaaa ccggttcgtt gcagggggta tataacctgg cgggattcat
    38281 taccacagcg agcgggcaac gaatggcgtt tgtacaatat ctttctggct atgcagtaga
    38341 accggcggat cagcgtaatc gccgtattcc gttagtgcgt tttgaaagcc gtttgtataa
    38401 agatatttat cagaacaatt agtcaaaaag aaaccccggc acatggccgg ggttttgctg
    38461 attaacgctt gtaaatgaac tcaacgcctt cttcgtcgtc ttcgtcccag tcatcatccc
    38521 agtcctcatc atcctcttca gcaatctctt caagctgctg gcgatgataa tcatcccaca
    38581 tgaattcgac tttctctggc tgtttcgctt cttcagcctg cacgaccggg ttttcaatga
    38641 taaaggtcat cacatcccag cagagatctt tcacgcccag tccactcgcc gcagagatca
    38701 gataatattt atcttcccag cccagcgctt cagcaatcgc tttcgctttc tcttcagctt
    38761 ctgccttatc cagcagatcg atcttattga acactaacca acgcggtttc gccgccagat
    38821 cctggctgta tttttccagc tcgctgataa taatacgcgc gttttcaacc ggatcggtgc
    38881 cgtcaatcgg atcgatatcg atgaggtgca acaggacgcg gcaacgttcc aggtgcttca
    38941 ggaagcggat acccagacct gcgccttccg cagcgccttc aatcagtcct ggaatatcgg
    39001 caacaacgaa gctcttttcg ttgtccattc gtaccacacc cagacttggc accagagtgg
    39061 taaacggata atccgccact ttcggtttag ccgccgatac cgcgcgaata aaggtcgatt
    39121 tacccgcgtt tggcatcccc aacataccga cgtcagccag cagcatcagc tccagcagca
    39181 gctcacgctt atcacccggc gtaccgttgg ttttctgccg tggtgtacgg ttaacggacg
    39241 atttgaaacg ggtattgccc agaccgtgcc agccgccctt agcaaccagc agacgctgac
    39301 cgtgtttagt catatcgccc atggtttcac cagtaccctg gtcgattaca cgcgtaccta
    39361 ccggcacttt aatcgtcacg tctttaccgc gcttaccggt acaatcgcgg cttgcgccgt
    39421 tctgaccgcg ctctgcacgg aaagattttt caaaacgata atcgataagc gtgttcaggt
    39481 tttcgtcggc ttccatccat acgtcaccac catcaccacc gtcgccgcca tccgggccgc
    39541 ctttcggaat atacttttcg cggcggaagc tcacgcaacc attaccgcca tcacctgcaa
    39601 cgaccagaat cgatgcttca tcaacaaact tcattttatt ctccgtaaat cattcgcctg
    39661 cgcggggttg cgataccacc gttgtatgct tacgtaatcc gccccaaata cgatgaccaa
    39721 tggcggaata catcgcgccc gcaaccacga caaacgcacc gagataacct aaaaggttta
    39781 acatcggtct ggcgaagaaa tcgggccagg ccagtgataa aagatctgaa aaaaacagcg
    39841 taaacagtgg ggtgagcgtg atgatcgcgc tcacctgcgc tgcctgccag cgagccatcg
    39901 cttccgccag ggcgccatat cctaccaatg tattcagtcc gcaaaaaatt aaacatgcga
    39961 gctgccagtg gctaagctgc gctatcactc ctggctttgc cagagggaag agcgcaattg
    40021 tacataaagt gtacagcaaa aacaggatct gcggtgaggc cagccgacgc aataaaacct
    40081 tttgcgccac gccataactc acccaaaccg tcgccgcacc gaccccaaag atgactcccc
    40141 aggtgtaatc agtgagcttt gtaaatatct cgaccagact ggtgttaaaa aacatcacca
    40201 ggccgctcag gagcatcaat gcccctacaa cctgagtgct gcgcattttc tctttcagga
    40261 taaatacgct ggcaaccatc atgccaactg gcgagagttg cccaatcacc tgcgaagcgg
    40321 tcggactcag gtattgcaag gatgagctga acaggatgaa gttcccaaac agcccggcgg
    40381 tcgccactgc caacaaaatc aaccagcgtg gcttacgaaa cacgcgtaat ggcggcaacc
    40441 tcttcttcac cgcaagaatg gcacccaggc caatactcgc catcaagaaa cggtaaaaca
    40501 cgattgtcgg aggttccatc acctccagca cctgcttcat tgcgattggc aacgcccccc
    40561 agcaaattgc tgtggtgagc gccaaaagaa tgccaatgcc tgcctgctgc ttcataccgt
    40621 attccctgca aagcgcattt gcggattaac gccgcaccat gctcattttt accggttatc
    40681 gaatgtaaaa agccccgcaa cgtgttgcgg ggctttcatc cgttaccggg acgcgaaaaa
    40741 cttattcagc ttcgatgctg ataaatttac ggtttttcgg gcctttaact tcgaatttca
    40801 ctttaccgtc tgctttagca aacagagtgt ggtcacgacc gcaacctacg ttagcgccag
    40861 cgtggaattt ggtaccacgt tgacgaacga tgatgctacc cgccagaacg gattcgccac
    40921 cgaaacgctt aacgcccagg cgtttagctt ctgaatcgcg accgttacgt gtggagccgc
    40981 cagccttttt atgtgccatt tgaaatctct cctcaggtct taggcgctga tgccagtaat
    41041 tttcacatca gtgaaccact gacgatggcc ctgctgctta cgatagtgtt tacgacgacg
    41101 aaacttaacg attttaactt tctcgccacg accgtgagca acaacttcag ctttgattac
    41161 gccgccatca acgaaaggaa cgccgatttt gacttcttca ccgtttgcga tcatcagcac
    41221 ttcagcgaac tcaacagttt cgccagttgc gatgtccagc ttttccaggc gaacggtctg
    41281 accttcgctt actcggtgtt gtttaccacc actttggaaa accgcgtaca tataaaactc
    41341 cgcttccgcg cacacctttt caatgattca gagtgcgcta taaatattca caatagggcg
    41401 cgaatattac gcaaaacgca cgcctttgac aagtgctaca gtcaatacac gaagaaaaaa
    41461 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatc accctatatt
    41521 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    41581 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    41641 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    41701 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    41761 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    41821 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    41881 accgccaacg ctgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    41941 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    42001 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    42061 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    42121 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    42181 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    42241 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    42301 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    42361 gccatcgaac agggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    42421 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    42481 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    42541 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    42601 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaaag ggcgatgaat
    42661 ctttatatat tctgaatatt cacactcttt acaggaactt tttagagcaa taggccatca
    42721 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    42781 gaggaaagat ggaaagtaat ttcattgact ggcatccccc tgacatcatt gcaggtttgc
    42841 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    42901 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    42961 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    43021 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    43081 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgcttaatat
    43141 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    43201 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    43261 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    43321 cggtattgct ttcgatttcg gcatgcgcgc ccatacggct aagctccggc acatgcataa
    43381 agcggttttc aaagaccgtt tcggtgatga atccggtccc ttctgccacc aggttcaaca
    43441 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    43501 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    43561 ccgctccagc gtcacgcagt ttcgccagca cggcgtcgag agtatctggc tgcgcgttac
    43621 ggcagataat tttgccgcga gaaatcgccg ccgccaccag gaaagtaccg gtttcgatac
    43681 gatccggtag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    43741 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    43801 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt accttccgcc agggttgcag
    43861 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    43921 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    43981 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    44041 gcagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagtgcc cagatagaag
    44101 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    44161 tatgcacaga accattacgt tctactttcg cacccagctg acttagcagc ttcattgatg
    44221 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagag
    44281 cggcaaaaag gataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    44341 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    44401 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    44461 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    44521 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcatgccg tcaaacaact
    44581 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    44641 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    44701 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    44761 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    44821 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    44881 tcgccagggt atacactttg tcgtttaccc cctgaagcgt cacattgttg ccctgctttt
    44941 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    45001 ggctaagatc gatgcaggta atccccttca ccgcttcctc acgcatttcc caaagcggta
    45061 gcaaaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    45121 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    45181 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    45241 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcctgcc agttgcccgt
    45301 ctgggagttt ttacgccact ggaagtccag acgcaccggc gggcggccat tcggatcaat
    45361 aatggtaacg cgaatcggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    45421 ataggtttga ccatgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    45481 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    45541 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    45601 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    45661 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    45721 actcagcggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    45781 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    45841 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    45901 aatgagatct tccagcacca tcgcagactt agtgtcctga attgtatcgc catccttcag
    45961 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttcccc
    46021 cagtaggccg gaagtacgaa tgctcagcga actggtatct ggaatgtggt tataacgttg
    46081 ttcaatttcc agcgttacgc gtggcagata ggttttcggg tccagcgtaa tatccgccac
    46141 ccggcccaca acaacgccac caatactgac cggagagcgc gctttcaggc cgccaatgtt
    46201 atcgaacgtc gcataaagcg tgtaggtcgg ttcagtacgt atggacgtca cgttcgccgc
    46261 cttcaggcaa acaaacagag ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    46321 ttcatttttt ttcgtttgca tgtactcaat tcccaaacat caatgcggtc agcacaaaat
    46381 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    46441 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    46501 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    46561 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagagatc
    46621 cgccccagat ccccacggcg acgaaaataa ccgtcaacag tggtaatgaa ataaccccag
    46681 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    46741 agagttgctc tgtagcgcgc atcaggccga tttctgcggt tagcgccgaa ccagcacgcc
    46801 cggcaaacaa caacgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    46861 gcatacccag actggtttcc gcactataag tggtcagaac cagataacct tgcagcccca
    46921 acaccattcc gatgaacacg ccagaaacca caataatcag catcgacagg acgccgacat
    46981 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    47041 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    47101 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    47161 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    47221 tgacgtacgc gcggatcagg attcgcctgc aacgcctggg cacagccatg agcgacaatt
    47281 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    47341 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    47401 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    47461 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    47521 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    47581 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    47641 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttgcgcact
    47701 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    47761 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    47821 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    47881 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    47941 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    48001 aacccaacat atttacagaa tattacccgc tgtggttagc gaaagctggc atttgtttta
    48061 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    48121 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    48181 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    48241 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    48301 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    48361 cgttggtaca gccctcggct caaacattat caatattttg ctgatcctcg gtctggctgc
    48421 gctggttcgt ccttttaccg tccattccga tgttctgcgt cgtgaattac ccttaatgtt
    48481 gttggtcagc gtagtggccg gttccgtact ctatgacgga caacttagtc gcagcgatgg
    48541 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    48601 ggctgaacgt caggggactg acagcctgac cagagagcag cttgcagagc tgccgcgtga
    48661 cggcggattg cccgtcgcgt ttttatggct cggcattgcg cttatcatca tgccagtggc
    48721 cacgcggatg gtggttgata acgccacggt gctggcgaat tactttgcca tcagcgagtt
    48781 gacgatgggt ctgacggcaa ttgctatcgg aaccagcctg ccggaactgg caaccgcaat
    48841 agcgggggtt cgcaaaggtg aaaacgacat tgctgtcgga aatatcattg gcgcaaacat
    48901 ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata acaccaggag agattgatcc
    48961 attggcgtac agtcgtgact acagcgtgat gttgctggtg agcattattt ttgcgttgct
    49021 gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg gtattattaa ctggcggatt
    49081 tatcgtatgg ctggcgatgt tgtactggtt atcgccaata ctcgttgaat aactggaaac
    49141 gcattatgtc gcacgtagag ttacaaccgg gttttgactt tcagcaagca ggtaaagaag
    49201 tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca atacatcaat cagaatttca
    49261 cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt tgtcgtcatg gggatgggga
    49321 aatcggggca catcgggcgc aaaatggccg caacgtttgc cagcaccggt acaccttcat
    49381 ttttcgtcca tcctggtgaa gccgcgcatg gtgatttagg catggtcacc ccacaggatg
    49441 tggtgattgc tatctctaac tcaggtgaat ccagcgaaat cacggcctta attccagtgc
    49501 ttaagcgtct tcacgtaccg ttaatctgca tcaccggtcg cccggagagc agcatggcgc
    49561 gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga agcctgtccg ttagggctgg
    49621 caccgaccag cagcaccacc gccacgctgg ttatgggcga tgccctcgct gtcgcgctgt
    49681 taaaagcacg cggctttact gctgaagatt ttgcgctctc acacccaggc ggcgcactgg
    49741 gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac gggcgatgag atcccgcatg
    49801 ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt tacccgcaaa aatcttggta
    49861 tgactgtcat ttgcgatgac aatatgatga ttgaaggcat ctttaccgac ggtgatttac
    49921 gccgtgtctt cgatatggga gtggatgttc gtcagttaag tattgccgat gtgatgacgc
    49981 cggggggaat acgcgtgcgc cctggcattc tggccgttga ggcactgaac ttaatgcagt
    50041 cccgccatat cacctccgtg atggttgccg atggcgacca tttactcggt gtgttacata
    50101 tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa ggataaacaa caatgagcaa
    50161 agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc gccgacgtta tagcaaaagc
    50221 agagaacatt cgtctgctga tcctcgacgt tgatggcgta ctgtcagatg gcctgattta
    50281 tatgggcaat aacggcgaag agctgaaagc gttcaatgtt cgtgacggtt atggcattcg
    50341 ttgtgcgctc acctctgata ttgaagtcgc tatcattacc gggcgaaagg ctaaactggt
    50401 agaagatcgt tgtgccacat tggggatcac tcacttgtat caggggcagt caaacaaact
    50461 gatcgccttt agcgaactgc tggaaaaact ggcgattgcc ccggaaaatg tggcttatgt
    50521 cggcgatgat ctcatcgact ggccggtaat ggaaaaagtg ggtttaagcg tcgccgtggc
    50581 cgatgctcat ccactgttga tcccgcgcgc cgattatgtg acgcgcattg ctggcggtcg
    50641 tggcgcagtg cgagaagttt gcgacttatt actcctggcg cagggcaaac tggatgaagc
    50701 caaagggcaa tcgatatgag taaagccaga cgttgggtta ccattgtgct atcactggcg
    50761 gttctggtga tgatcggcat taatatggcc gaaaaagacg ataccgccca ggtggtcgtc
    50821 aacaacaatg atcccaccta taaaagcgag catacggaca cgctcgtcta taacccagaa
    50881 ggggcactaa gctatcgatt gattgctcaa cacgttgaat attattccga tcaggccgtt
    50941 tcgtggttta cgcagccggt acttaccacg tttgataagg ataaaatccc gacatggtcc
    51001 gtaaaagcag ataaagccaa gctgaccaat gaccggatgc tctatttata tggacacgtt
    51061 gaagtcaacg cacttgtgcc ggactctcaa cttcgcagaa tcacgacgga taacgcgcag
    51121 atcaatctgg tgacgcagga tgttacctct gaagacctcg tcacgttata cggaacaaca
    51181 tttaactcca gcggtctgaa aatgcgcggc aacttacgca gcaagaacgc cgagctgatt
    51241 gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa ctcagcctta atcttgtgct
    51301 tgccagctca cttctggccg ccagcattcc ggcatttgcc gtaaccggag acactgatca
    51361 gccgatccac attgaatcgg accagcaatc tcttgatatg caaggcaacg tggttacctt
    51421 taccggtaat gtcatcgtca cccagggcac catcaaaatt aatgccgaca aagtggtcgt
    51481 tacccgtccg ggcggcgaac aaggtaaaga agtgattgac ggctacggta aaccggcaac
    51541 gttctaccag atgcaggaca acggtaaacc cgttgaaggt catgcttccc agatgcacta
    51601 cgaactggca aaagattttg tcgttctgac gggtaatgct tatctgcagc aggtcgatag
    51661 caacattaag ggcgataaga tcacttacct ggtgaaagag cagaaaatgc aggctttcag
    51721 cgacaaaggc aagcgcgtaa caaccgttct ggtgccgtcg cagctgcagg acaaaaacaa
    51781 caaaggccag accccggcac agaagaaggg taattaattc gttatggcaa cattaactgc
    51841 aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta gaagacgtca gcctgaccgt
    51901 caactccggg gaaattgtcg gtctgctggg gccaaacggt gccggtaaga ccaccacttt
    51961 ctatatggtt gttggcattg tgccgcgcga tgcgggcaac atcattattg atgatgacga
    52021 tatcagtctg ctgcctctgc atgcacgcgc gcgccgcggt atcggctatc tgccacagga
    52081 agcctccatt ttccgtcgcc tcagcgttta cgataacctg atggcggtac tgcaaattcg
    52141 tgacgacttg tctgctgaac aacgtgaaga ccgcgcgaac gagctgatgg aagagtttca
    52201 cattgagcac ctgcgtgaca gcatggggca gtcactctcc gggggtgaac gtcgccgtgt
    52261 agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt ctactcgacg aaccgtttgc
    52321 cggggttgac ccgatctcgg ttatcgacat taaacgcatc attgaacacc tgcgcgacag
    52381 cggcctgggc gtactgatca ctgaccacaa cgtgcgtgaa acactggcgg tttgtgaacg
    52441 cgcttatatc gtcagtcagg ggcatttgat tgcccacggc acgcctacag aaatcttaca
    52501 agacgaacac gttaagcgtg tataccttgg ggaagacttc agactctgat agggtagaag
    52561 tttgcgacgt tttagcagga gagtacgatt ctgaacatga agcaaggttt gcaactcagg
    52621 cttagccaac aactggcgat gacgccacag cttcaacagg cgattcgtct gttgcagttg
    52681 tcgacgctgg aacttcagca ggagctacag caggcgctgg agagtaatcc gctgcttgag
    52741 caaatcgaca ctcatgaaga aatcgacacc agcgaaacgc aagacagtga aacgctggac
    52801 accgccgacg cgctcgaaca aaaagagatg ccggaagagc tgccgctcga tgccagttgg
    52861 gacaccattt acaccgctgg tacaccatcc ggcaccagcg gtgactacat tgacgatgag
    52921 ctgccggtct atcagggcga aacgacgcag accttgcagg attacctgat gtggcaggtc
    52981 gagctgacac cgttttccga cactgaccgc gctattgcta cctctatcgt cgatgccgtt
    53041 gatgacaccg gttatctgac tgtcccgctg gaagatattc tcgaaagtat gggcgatgaa
    53101 gagatcgaca tcgacgaggt tgaagccgtc cttaagcgga tccaacggtt tgatccggtc
    53161 ggtgtagcgg caaaagatct gcgtgactgc ctgctaatcc aactctccca attcgataaa
    53221 accacgccgt ggctggaaga ggccagactg atcattagcg atcatctcga tctgttagcc
    53281 aatcacgact tccgcacttt aatgcgcgtc acgcgtctga aagaagatgt gctgaaagaa
    53341 gccgtcaatc tgatccagtc gctcgatccg cgccccgggc agtcgatcca gactggcgaa
    53401 cctgagtatg tcattccaga tgtgctggtg cgtaagcata acggtcactg gacggtagaa
    53461 ctcaacagtg acagcattcc gcgtctgcaa atcaaccagc actacgcctc gatgtgcaat
    53521 aacgcgcgca acgatggtga cagccagttt atccgcagca atctgcagga tgccaaatgg
    53581 ttgatcaaga gtctggaaag ccgtaacgat acgctactgc gcgtgagtcg ctgtatcgtt
    53641 gaacagcagc aagccttctt tgagcaaggt gaagaatata tgaaaccgat ggtactggcc
    53701 gatatcgccc aggctgtcga aatgcatgaa tcgacgatat ctcgcgtgac cacgcaaaaa
    53761 tacctgcata gtccacgagg catttttgaa ctgaagtatt tcttttccag tcacgtcaat
    53821 accgagggcg gcggcgaagc ttcctccacg gcgattcgtg cgctggtgaa gaaattaatc
    53881 gcggcggaaa acccagcgaa accgttgagc gacagcaagt taacctcttt gctgtcggaa
    53941 caaggtatca tggtggcacg ccgcactgtt gcgaagtacc gagagtcttt atccattccg
    54001 ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat aaggaagaca ctatgcagct
    54061 caacattacc ggaaataacg tcgagatcac cgaggcactg cgcgaatttg ttagagccaa
    54121 atttgccaaa cttgagcaat attttgaccg aatcaaccag gtctatgttg ttctgaaagt
    54181 ggagaaagtc acccacacct cagatgcaac actgcatgta aacggtggtg aaattcatgc
    54241 cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc ttaattgata agctggcacg
    54301 ccagttgacc aaacataaag ataaactgaa acaacactaa ttgtccgggc aattagcatg
    54361 tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta cggttaatgc tccgagcctg
    54421 ttccactgtt tgagtgggca ggttcttagg tgaaattatg acaaataatg atacaactct
    54481 acagcttagc agtgttctta acagggaatg tacgcgaagc cgcgttcact gtcagagcaa
    54541 aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa caacttagcc tgccgcctca
    54601 ggtggttttt gaagctatcc tgacgcggga aaaaatgggc agtaccggta tcggcaatgg
    54661 tattgccatt ccgcatggca aactggaaga agatactctg cgcgccgttg gcgttttcgt
    54721 tcagctcgaa acgcctatag ctttcgatgc catcgacaac caaccggtgg acttactttt
    54781 tgccctgctg gtgccggcag accaaactaa aacgcacctg catactctgt cgctggtggc
    54841 gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc gcagcccaga gcgatgaaga
    54901 gctgtatcaa atcattacgg ataccgaagg tactccggat gaagcgtagt tattcggtaa
    54961 tgtctctttt agacgttgtg aggagaaaca gtacatggta ctgatgatcg tcagcggacg
    55021 ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa gatatgggtt tttactgcgt
    55081 ggataacctt cccgtagtgt tgttacccga tctggctcga accctggccg atcgcgagat
    55141 ttctgccgcc gtcagcattg atgttcgtaa tatgccggag tcaccagaaa tattcgaaca
    55201 ggcgatgagt aacctgcctg acgctttctc accgcaactc ctgttcctgg atgccgaccg
    55261 taatacctta attcgtcgtt acagtgacac gcgccgactg catccgcttt ccagcaaaaa
    55321 cctgtcgctg gaaagtgcta tcgacaaaga aagcgatttg ctggagcctc tgcgttcgcg
    55381 agcggatctg attgtcgata cctcagaaat gtccgttcac gagctggctg aaatgctgcg
    55441 tacccgtctg ctgggtaaac gtgaacgcga actgaccatg gtctttgagt ctttcggctt
    55501 caaacacggt atccctatcg atgcagatta cgtctttgac gtgcgcttct tgccgaaccc
    55561 gcactgggat ccgaaactgc gtccaatgac aggtcttgat aaacctgtcg ccgcgttcct
    55621 cgaccgccac acagaagtac acaattttat ctaccagacg cgaagctatc ttgagctatg
    55681 gttacctatg ctggaaacca acaaccgtag ctacttgacg gtcgccattg gttgtaccgg
    55741 cgggaagcac cgttcggtgt atattgcaga gcaactggca gactacttcc gctcgcgcgg
    55801 taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt aaaccatgac cgtcaagcaa
    55861 actgttgaaa tcacaaacaa gctgggcatg catgcccggc ctgcaatgaa gctgtttgaa
    55921 ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg acgaaggcac cgaggccgaa
    55981 gccaacagcg ttattgcgct gctgatgttg gattctgcca aaggacggca gattgaagtt
    56041 gaagcgaccg gcccacagga agaggaagca ctggccgccg ttatcgccct ctttaactct
    56101 ggttttgatg aagattaatc ttcatcactt tgacatacaa ctcccttcaa actccccccg
    56161 acaataagaa aatcacgtac tgaaatcgtt ctcaatcaac gtcatttgta cattttgtgc
    56221 gcttttcact ttcagaagaa ccttaagaaa accttaagag gcattgttta ggttttgttt
    56281 aagttaatcg accatactgg agatcgtcag aaaatatttc caggagatgg catgattcgc
    56341 ttatctgaac aaagtcccct gggcaccgga agacatcgga aatgttatgc gcatccggaa
    56401 gatgcccaac gctgtatcaa gattgtctac catcgtggcg atggtggcga taaagagatc
    56461 cgccgtgagt taaagtacta cgcgtatctt ggtcgccggt taaaagactg gagtggaata
    56521 ccgcgctatc acggtaccgt tgagacggat tgcggaaccg gttatgtcta cgatgtgatt
    56581 gcagattttg acggcaaacc ctccattacg ctgaccgaat ttgctgaaca atgccgttac
    56641 gaagaagaca ttgcacaact gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat
    56701 aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc tctgtcacca catcagtgag
    56761 tcagaggtta tcccggtcgt ctgcgacaac attggtgaaa gcacattgat tccactggcg
    56821 acctggtcga agtggtgttg tctgcgtaag caagagcggt tatggaaacg atttatcgcc
    56881 caaccggcgc tggctattgc cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg
    56941 gccctcactt cgcgcgaagc ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct
    57001 aactgataca tctgccgtaa aatccacgcc tgacggctac gcacgtagcc cgatggcgag
    57061 gagactttga aacgaagtgg attaggtaat acagctgcca gtaatgcagc ttccgaccgg
    57121 gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg cagcttcgac gccaaacaca
    57181 ccgtcgccaa attcggcgat attcaggtaa accgtcagga tacgcttttt gctccagact
    57241 gtttctatcc ccagcgttaa tccagcttcc agcccttttc gtacccagct acgcccatcc
    57301 cataaaaaga gatttttggc cgtctgctga gaaatcgttg aagcaccgcg aatacggttt
    57361 tcattgcgct cgttgtgcgc cagggctttc tcaatggaag cgacatcaaa gccccggtgc
    57421 tcaggaaatt tctgatcttc tgcggcaatc accgccagtc ccatccacgg cgagatttga
    57481 tccatactga cccagtcaga atgtgccacg taacgaaaat tgccatgcag ccaggcgctg
    57541 acctgtcgct cgaccattac cgctgagaag ggaacaggcg caacactaaa caacgcgatg
    57601 cccccgcccc agaaaacggc gaggacaacc attaaccgca aaagaaaacg gcgaacgaaa
    57661 ctaaacaccg ttaagcggct tttactcatt cagccagaac cagcacgcgg gaaaccagct
    57721 tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag catatatgct ggggtggtga
    57781 caatcttatt gtcttcatca accacgatat catcgacagg acagggcaca tgctccgcgc
    57841 ccatctcttc cagcacttct gcggtatcga tatcagtacc gatggtcaaa cgcagcggga
    57901 aatcgaaaat tttcggcagc atcgccgggg caatacacat aaaaccaagc ggttttccgg
    57961 cctgatgcat cgcttgtgcc agcgccttta attcacggtc aacggtgcat tcgctaccaa
    58021 gagtggcaaa attgcttaaa ttcttcgccg cgccaaaccc ccccggcaca atcaacgcat
    58081 ccagttcagc ggcatcggcc tgggccagag gacggatttc accgcgcgtt attcgtgccg
    58141 cttcaatcag cacgttgcgc gtttccgtca tcgcttcgcc agttaaatgg ttgataacat
    58201 caacctgctg cttatccggt gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta
    58261 acaacgtcaa caccgcttca tgaatttcag aaccgtcata gacgccgcat ccgctcagaa
    58321 ttacgccaat tttcttcatt gtgatcatcc tttcgcaatt gactgaaaca cattaatttt
    58381 tttaataaaa atggtacgca tcacacattt aactgattca tgtaacaaat catttaagtt
    58441 ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa
    58501 taatttacgg cgcagccaag atttccctgg tgttggcgca gtattcgcgc accccggtct
    58561 agccggggtc attttttagt ggcttttgcc acccacgctt tcagcacttc tacgtcgtga
    58621 cgccactctt ctttcatctc ttcaatccat tcaccgacgt tatcttccca ggcaggaagg
    58681 tcaggagact gaatttgctg acccagctgt tgcagatggc gtaaccccac tgaccccgcc
    58741 gcacctttaa ttttatgtcc ttcctcaaca atgccttttt tatcctgcgc cgtcagattc
    58801 gactccagca cgctgacata gcccggcatc atcttctcaa acaccgctaa cccgtcggtg
    58861 atcagcttcg gtcctacaag ttcgagatac tgttccagca tgggaatatc gagcaatgct
    58921 tctgatttac tgttctcttc tgtcgtcacc gtactctcct catcatcctg ggtatcccag
    58981 aatttcttga tcatcgcggt tagcgccgga accgaaagcg gcttactcag cacatcatcc
    59041 attccagcat tgaggtactc ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc
    59101 ggcggtaaat cctcgcgcgg ataacgcttc gtcagttcac gagagatatc cagcccggtc
    59161 atatctggca actgaatatc cagcaacacc aggtcgtatt cgcccggttt aaacatctcc
    59221 agcgccgcct tgccggtcat ggcgacatca acgctgttac ctaatttttc cagcacagaa
    59281 cgcgcaacaa tcacgttcag ttcaatgtct tccaccagca gcacattcag cgccggtaaa
    59341 ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg ctaccgacgg tgcgtggatc
    59401 gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa ccgtaatatc gccgcccata
    59461 tttttcgcca gacgacgaga aacggccaga ccaataccgg tgccggtggc aggtttaccg
    59521 ccatgactgt ctttcacctg gtaatacatg gcgaaaattt tatccagctc atcctgcgga
    59581 atgccgatac cagagtcttc cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg
    59641 cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt tactgatgag gttccacagg
    59701 atctgccgta aacgcgtccc gtcggtaatg acctgatgcg gtaatggcag cgtcggctcc
    59761 aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg agagattttc cagatcggca
    59821 aggaagctgg tgaaatcaac cggttgatta tcaagctgga ccttgcgccg ttccatctta
    59881 tccatgtcga taatatcgtt aaagatattc cccagcgtga cggccgaaac atggatggtc
    59941 ttgagatatt tttcctgctc ggcggtgagt tcggtatcca gcagaatgcg gctcagaccg
    60001 acgataccgt tcagcggtgt acgcaattcg tgactgatgg tggagataaa cgtcgttttg
    60061 tcgcggctgg cccgttcaag cgcatcctga taccgcttac gctcggtaat gtcgcgacca
    60121 aagcccatca aaccgtgacg tttacccacg cggtcgtagt acggcacttt acggatttca
    60181 aagcaggctt tgcgcccgtc cgggtaatcc agccactgtt cataggtcag tgacacatta
    60241 tgacggaaca ctttttcatc ggtttcaatg acttttgcgg cggcttccgg tgagtaaaca
    60301 tcagcaggtt tcaggtgaac cagttgtttt tcgctttttc cggtcagcag ctccatcgcg
    60361 cggttacagc cggaaaactc tttatcttcg ttacgataaa aaaccaggtc gggtgaagca
    60421 tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc
    60481 tctttgattt caattttcag ttggccgaag gtttcctgca gttctgcttc cgctttttca
    60541 cgaacggcaa tttcctgatt tagctgggca atattatctt ttaactgaac gttgaggctc
    60601 aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc gtgacagacg ttgtcgtgac
    60661 tcctccagtt gctcgacgac caccgataga aagtagaccg cccacggcgt aatcagcaaa
    60721 ccaaagaaga tagaacgaat aacatcaatg ctttcgacct gaccatgcag caccatggtt
    60781 accgccattt gtaccacaat ggcaagaacg acgagggcca gcgccagcaa cattgagaag
    60841 cgcaccagac ctaacttcat catcaggtca acataatact gcgccagcag acgaatttgc
    60901 ttcattaggg aattccttca cgacaacctg ccccaataat acccaattct gacagttacg
    60961 ttataaattg tgtgagaaat gcggaattcc cctcaacgac ctactccgtt ggaggtagcc
    61021 acggacgtcc cagcgccgat ccctgtacgc catgttcatt cagatagcga tccagctcga
    61081 ccattcccgt ccatcgattt tcgcaccaca gcggagcaag cagcgtcggg cgacgggcgc
    61141 tggcggaaat gcggtggtag atcacttccg gtggcgtatg gcgaatcatt tctccggcag
    61201 tgagcgtgta atcctccagt tcaataccgt tcaaacgccc cgcttcccag gctttcgcca
    61261 taatgctgcc tttcacaata tgcagcggat gcagctttat gccgtccacg ccggtttcaa
    61321 ccactcgctc cagcgtttgc aaacattcag cctgcccttc accaggcagg ccgacaatta
    61381 agtgggaaca caccttcagc ccgcgctgac gtgccagctg ggttgtacgc tgataacagg
    61441 caaaatcatg gccgcggttg atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca
    61501 gctccagcca cacttcgtaa ccctggtcct tatattcgca aagcagatcc agcaccgcat
    61561 ccggcacgca gtccgggcgg gtaccaacac acaaaccgac aatattggcc tggctcaccg
    61621 cctgctgata catagaacgc agcacctgaa cttccgcaaa ggtgctagta tacgcctgaa
    61681 agtaggccag atagcgttta gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg
    61741 caatggaacg atgctgctgc gcttcatcgg caaacgaggc aacattacag aatgtgcagc
    61801 caccacgccc gatggtaccg tcacggttag ggcagctaaa accgccgtgc agcgtcagct
    61861 tatgcacctt ttgcccataa cgacgggtga gatcaccacc aaacatattg actaatttct
    61921 gtaactgcat aatctgatag accgcgcctt gaaaagaggc caaagcctgc catttttagc
    61981 tcaattcggc gatgacctgg atcaatcgtc ccggcctgct tttatcaact gcataatcaa
    62041 tcaaaattac cgaaatttca tgcataatca cataaatcac ttttgcttat cttgtgtcag
    62101 atttttttat ctccttatgg attttaggca aaaacagtag catgaaacgt cattaccaat
    62161 taaggcagta taaaatgctg gttttctcgt cagttcaagg taggataagg gttaacacgc
    62221 ctttatgaca gtcaggaatt gactgtttct ctaacgactt cccttttagc cttaaagata
    62281 aaattcattt taatttcagt tgcttaataa agaattttgc gctaaagcac atttctgtac
    62341 caataagctt gccatttgac ctgtatcagc tttcccgata agttggaaat ccgctggaag
    62401 ctttctggat gagcagcctg ctcatcatat ttatgcagta attgagatcc cctcttcacc
    62461 gtattaaccg atgcgaaaag gacaacaagg gggcgaatac gaggcgcgcg tatgacacgc
    62521 aacccccgtc gccacgctct ttctgtgccc gtgcgcagcg gttcggaagt ggggttcccg
    62581 cagagcctgg gggaggttca cgatatgttg tacgataaat cccttgagag ggataactgt
    62641 ggtttcggcc tgatcgccca catagaaggc gaacctagcc acaaggtagt gcgtactgca
    62701 atacacgcac tggcccgcat gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggt
    62761 gacggttgcg gcttgctgtt acaaaaaccg gatcgctttt ttcgcatcgt tgcgcaggag
    62821 cgcggctggc gtttagcaaa aaactacgct gtcgggatgc tcttcctgaa taaagatcct
    62881 gaactcgcgg ctgccgcacg ccgcatcgtt gaagaagagc tgcaacgcga aaccttgtcg
    62941 attgtgggct ggcgtgatgt ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc
    63001 tctctgcccc gcattgagca aattttcgtt aacgctccgg caggctggcg tccgcgggat
    63061 atggagcgcc gtctgtttat cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa
    63121 gacttctacg tctgtagcct gtcgaatctg gtgaacatct ataaaggtct gtgtatgccg
    63181 gcggatctgc cgcgctttta tctggatctt gcggacctgc gtctggaatc ggccatctgc
    63241 ctgttccacc agcgcttctc cactaacacc gtaccgcgct ggccgctggc gcagccgttc
    63301 cgctacctgg cgcataacgg tgaaatcaac accatcaccg gtaaccgcca atgggcgcgt
    63361 gcgcgtacct ataaattcca gacgccgctt atccctgatc tgcacgacgc cgcaccgttc
    63421 gtcaacgaaa ccggctctga ctccagttcg atggataaca tgctggaact gctgctggca
    63481 ggcgggatgg atatcatccg cgccatgcgt ctgttagtgc cacccgcctg gcagaacaac
    63541 ccggatatgg acccggaact gcgtgccttc tttgacttta actccatgca tatggagccg
    63601 tgggatggcc cggcgggcat cgtgatgtcc gacggtcgtt ttgccgcctg taacctcgac
    63661 cgtaacggtc tgcgtccggc gcgctacgtc atcaccaaag ataagctcat cacctgcgcc
    63721 tctgaagtcg gtatctggga ttaccagcct gacgaagtgg tcgaaaaagg ccgcgtcggg
    63781 ccaggcgaac tgatggttat cgacacccgc agtgggcgta ttctgcactc ggcagaaacc
    63841 gatgacgatc tgaaaagccg ccatccatat aaagagtgga tggagaaaaa cgtccgcaga
    63901 ctggtaccgt ttgaagatct gcccgatgaa gaagtgggta gccgcgaatt ggacgacgac
    63961 acgcttgcca gctaccagaa acagtttaac tacagtgcgg aagagctgga ctccgtaatt
    64021 cgcgtactgg gcgaaaacgg tcaggaagcg gtcggttcga tgggcgatga taccccattc
    64081 gccgtgctct ccagtcagcc gcgcattatt tacgactact tccgccagca gtttgcccag
    64141 gtgactaacc cgccaatcga cccgctgcgt gaagcgcatg ttatgtcgct cgccaccagt
    64201 atcggtcgtg aaatgaacgt cttttgcgaa gcagagggcc aggcgcaccg tttaagcttt
    64261 aaatcgccga ttctgctcta ctccgatttc aaacagctca cgacgatgaa agaggagcac
    64321 taccgcgcag atacgctgga tatcaccttt gacgtcacta aaaccacgct cgaagcgaca
    64381 gtcaaagagc tgtgcgacaa agccgaaaaa atggtacgta gcggcaccgt gctgctggtg
    64441 ctctccgacc ggaatatcgc taaagatcgc ctgccggttc cagccccgat ggcggttggc
    64501 gcgatccaga cccgtctggt cgatcaaagc ctgcgttgcg atgccaacat catcgtcgaa
    64561 accgccagcg cccgcgatcc gcaccacttc gccgtgttgc tgggcttcgg cgcgacggct
    64621 atttatccat accttgccta tgaaacgctg ggccgcctgg tagacaccca tgcgattgcc
    64681 aaagattatc gtaccgtgat gctcaactac cgtaacggca tcaacaaagg cttgtacaaa
    64741 atcatgtcca aaatgggcat ctccaccatc gcctcttacc gctgctcgaa actgtttgaa
    64801 gcggtcggtc tgcacgatga tgtagtgggc ctgtgcttcc agggggcggt cagccgcatt
    64861 ggtggggcga gctttgaaga cttccagcag gatctgctga acctgtcgaa acgtgcctgg
    64921 ctggcgcgta agcccatcag ccagggcggc ctgctgaaat acgtccacgg cggcgaatac
    64981 cacgcttaca acccggacgt ggtgcgcacg ctgcaacagg cggtacaaag cggcgagtac
    65041 agcgactatc aggaatacgc gaagctggtt aatgagcgtc cggcaaccac gctgcgcgat
    65101 ctgctggcaa ttacgccggg tgaaaacgcg gtcaacattg ctgatgttga accggcaagc
    65161 gaactgttta aacgctttga taccgccgcg atgtctatcg gcgcgttaag cccggaagcc
    65221 cacgaggcgc tggcggaagc gatgaatagc atcggcggta actcgaactc cggtgaaggc
    65281 ggcgaagacc cggcgcgcta tggcactaac aaagtgtcgc gcatcaagca ggtggcttct
    65341 ggtcgcttcg gcgttactcc ggcgtatctg gtcaatgccg acgtcattca gattaaagtc
    65401 gcccagggcg cgaagccggg cgaaggcggt cagttgccgg gcgacaaagt cactccttac
    65461 atcgccaaac tgcgctattc ggtgcccgga gtgacgctga tctccccgcc gccgcaccac
    65521 gatatctact ctatcgagga cttagcgcag ctcattttcg acctcaagca ggttaacccg
    65581 aaagcgatga tctccgtgaa gctggtttcc gaaccgggcg taggcaccat cgcgactggc
    65641 gtggcaaaag cttatgccga tttaatcacc atcgcaggct atgacggtgg caccggcgca
    65701 agcccgctgt cgtcagtgaa atacgcaggt tgcccgtggg agctggggct tgttgaaacc
    65761 cagcaggcgc tggttgccaa tggtctgcgt cataagatcc gtttgcaggt cgatggcggc
    65821 ctgaaaaccg gtgttgatat catcaaagcg gcgattctcg gcgcagaaag cttcggcttc
    65881 ggcactggcc caatggtcgc gctcggctgt aaatatctgc gtatttgcca cctgaacaac
    65941 tgcgcaacgg gtgtggcaac tcaggatgac aaactgcgta agaatcacta tcacggcctg
    66001 ccgttcaagg tgacgaatta ctttgagttt atcgcccgtg aaacccgcga gctgatggca
    66061 cagctggggg taacgcggct ggtggatctg attggtcgca ccgacctgct gaaagagctg
    66121 gacggtttca ccgccaaaca gcagaaactg gcgctgtcga agctgctgga gaccgccgaa
    66181 ccgcatccag gtaaggcact ctactgcacc gaaaacaacc cgccgtttga taacggcctg
    66241 ctgaacgcgc agttgctgca acaggcgaaa ccgtttgtcg atgaacgcca gagcaaaacc
    66301 ttctggttcg atatccgcaa taccgatcgc tccgtgggcg cgtcgctttc aggctatatc
    66361 gcccagacgc acggcgatca ggggctggca gccgatccta tcaaagcgta cttcaacggc
    66421 accgcaggcc agagtttcgg tgtatggaac gcgggcggcg tggaactgta cctgaccggg
    66481 gatgccaacg actatgtcgg taaaggcatg gcgggcggct taatcgccat tcgtcctccg
    66541 gttggttccg ccttccgcag ccatgaagca agtattatcg gcaacacctg cctgtatggc
    66601 gcgaccggtg gtcgtctgta tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcggaac
    66661 tccggtgcta tcaccgtggt agaaggcatt ggcgacaacg gctgtgaata tatgacgggc
    66721 ggtattgtct gtattctggg taaaaccggc gttaacttcg gtgcgggcat gaccggcggc
    66781 ttcgcttacg ttctcgatga aagcggtgat ttccgcaaac gcgttaaccc ggaactggtc
    66841 gaggtcttaa acgttgacga tctggcgatc catgaagagc atctgcgcgg tcttatcacc
    66901 gagcatgttc agcatactgg ctctcagcgc ggtgaagaga ttctggcgaa ctggtcaacc
    66961 ttcgccacta aatttgcgct ggttaaaccg aagtccagtg atgtaaaagc actgctgggt
    67021 caccgtagtc gtagcgcagc agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg
    67081 agtcagaatg tttatcaatt tatcgacctg cagcgcgttg atccgcctaa gaaaccgctg
    67141 aagatccgca aaattgagtt tgttgaaatt tacgagcagt tttccgaagg ccaggccaaa
    67201 gcgcaggctg accgctgcct gtcgtgcggc aacccatact gcgagtggaa atgtccggta
    67261 cacaactaca tcccgaactg gctgaagctc gccaacgagg ggcgtatttt tgaagcggcg
    67321 gaactgtcgc accagaccaa caccctgccg gaagtttgcg gacgagtctg cccgcaagac
    67381 cgtctgtgcg aaggttcctg cactctgaac gatgagtttg gcgcggtgac catcggcaac
    67441 attgagcgtt atatcaatga taaagcgttc gaaatgggct ggcgtccgga tatgtccggc
    67501 gtgaagcaaa ccggtaaaaa agtggcgatt atcggcgcag gcccggcagg tctggcgtgt
    67561 gcggatgtcc tgacgcgtaa cggcgtaaaa gccgttgtct tcgaccgtca tccagaaatt
    67621 ggcgggctgc tgaccttcgg tattccggcc ttcaagctgg aaaaagaggt aatgacgcgt
    67681 cgccgtgaaa tcttcaccgg catgggtatt gaattcaaac tcaataccga agtgggccgc
    67741 gacgtgcagc tggacgatct gctgagtgat tacgatgccg tcttccttgg cgtcgggact
    67801 tatcagtcaa tgcgcggcgg gctggaaaac aaagacgccg atggcgtgta cgcagcgctg
    67861 ccgttcctta tcgccaacac caaacagtta atgggctttg gtgaaacccg cgacgaaccg
    67921 ttcgtcagca tggaaggcaa acgcgtggtg gtccttggcg gtggcgacac tgcgatggac
    67981 tgcgtgcgta cgtccgtgcg ccagggagcg aagcacgtta cctgtgccta tcgtcgtgat
    68041 gaagagaaca tgccgggttc ccgccgcgaa gtgaaaaacg cgcgggaaga aggcgtagag
    68101 ttcaaattca acgtccagcc actggggatt gaagtgaacg gtaacggcaa agtcagcggc
    68161 gtaaaaatgg tacgtaccga aatgggcgaa ccggatgcca aaggccgtcg ccgcgcggag
    68221 atcgttgcag gttccgaaca tatcgttccg gcagatgcag tgatcatggc gtttggtttc
    68281 cgtccacaca acatggaatg gctggcaaaa cacagcgtcg agctggattc acaaggccgc
    68341 atcatcgccc cggaaggcag cgacaacgcc ttccagacca gcaacccgaa aatctttgct
    68401 ggcggcgata tcgtccgtgg ttccgatctg gtggtaaccg ctattgccga aggtcgtaag
    68461 gcggcagacg gtattatgaa ctggctggaa gtttaagctc tgtcccagcg ccgcctcctc
    68521 agaaaagagt cggcgctgaa attctccttt ctttctggaa agcgcctcgc ataatctgaa
    68581 gaaaaccaat gtaatacgct catctggatt gttaccggta gatgcccggc gtatagctga
    68641 ttccattatt caacgcagcg ccatataaag ggacagataa tggaatcgct ctcagagaga
    68701 acctcaacag gctaccagca aatccacgac ggtattattc acctggtcga tagcgcccgg
    68761 acggaaacgg tacgtagcgt taacgcgctg atgaccgcga catactggga aattggccga
    68821 cgaattgtcg aatttgaaca aggtggcgag gcaagggctg cgtatggtgc gcagctaatc
    68881 aagcgactat caaaggattt aagtctaagg tataagcgtg ggttctctgc aaaaaactta
    68941 cgccaaatga ggctttttta cctctttttt caacatgttg aaattcgcca gacagtgtct
    69001 ggcgaattaa caccattggg aattccgcag acaccatctg cagaattccc atcggcaaag
    69061 atttggcaga cgctgtctgc caaattgaga ggctgtttga tatttccaca gcttttgtat
    69121 tgcatctcct attttcggtt tgtatattca tgcaaataaa cgaaggaaag ttgccatgat
    69181 ttacattgaa cagcttgaat taatccataa aagtggtgat gtgttgtatc cagtaaaaat
    69241 aaccagaaaa tcatcaggaa aaactgcctt ccatcttgtt ccattcggct taaataagac
    69301 tcacgacctt ctagaggtgg aagatgcttc agaagcaatt cggctggtga ttgatgaacg
    69361 tcattccatt cgctgcagta cacttacagc aactattacg aataaaaagg gcaagcgaat
    69421 taagcggaca ggtatataca gtatcaaggg tgttaacata aaagaataca acgtcaggta
    69481 atggcacaga gcggtccgtc agttcaggtt tggctctgtg ccaaagatgg gtcaggtcac
    69541 acctgatcta ataaatttaa gccattaaat cagccttaac cttcaccacc acctttttaa
    69601 tctcacccgg ctcgccgaca acgcatcctg gtttatgcgg ttcacttggc ataaacacgg
    69661 cgaacattcc cggttttaag atgatggctt gctcgttatc aatggtgctg caaagctgat
    69721 aatcatcctc atggtggaac tcttcacact ggcgcgcagt gcctgccatg ccaaacagaa
    69781 tccgttcctc accgtttaat aacagctgga tatcaatgta ttgctcgtgc aattccgctt
    69841 ttttctcgac gggcgattga gtgttaaacg tcatgacatt cataaagata ttgtcgccct
    69901 gtaattcgta acgaccaggc gctttttctt gcggtctggc agctaatgcc agcgttaacg
    69961 cgtcctgtaa cgcaggatgt aacccagcag acggtaatga ctgtacttca cccatcatca
    70021 taatttttct ccctgggcca acagcgcagc cccaagtaaa cctgcatcgt ggcggtagtg
    70081 cgccgccagt aaatcaacat gaaatgccgc tggctcctgc gccagatacg tttccaccaa
    70141 cgccagatac ccttctgcca gaccaacgct gccaccgacc accacgcact ggcaatcagt
    70201 tgtggcttta atatcagcga tcagccttgc cagcacatgt gcggagcggt gaatcagttg
    70261 ctgcgcctgc tcgtcgccct gcccggcgcg tgtgaaaata gttctcgcat ccgcgcccat
    70321 gagttcacct tgcgctgccg ctgcaatacc gcgaccagaa gcaatcgctt ccacgcaacc
    70381 tgtgcgtcca cagccgcaga ctgggccgtg tggatcggca agcgtatgcc cgatgtgccc
    70441 cgccagaccg ccagggccgg taagcagttt accgccgctc actacaccgc cgccgacgcc
    70501 agtggaaacg gtgataaaca ccatatcggt tatatcgcct tccagcgcct gatactctgc
    70561 ccatgctgcg gcctgcgcgt cgttaatggc aatggtcggc aaattggaaa gttgttccag
    70621 cgttttgact aacggaaagt gtagcaatcc accaagatta tgcggattca gcgccagcaa
    70681 gctgccgtcg cgaataattc cggttgaagc gatggcaacc tgctgcgcat gagcttgcaa
    70741 cggggcgact aacgcggcta aggcatcacg caacgcctgc ggagtctggc tggctggtgt
    70801 cggaagttct cggcgatcgc ggatctgccc gtcagcgcca atcagcgcgg cggcaagttt
    70861 agtaccgccg atatcaatcg ccaacgtggt catagcaccg cctttttcat cgctgtgttg
    70921 taccactgac aaatgtgttc aagacgcgta atggcagaac cgaccgtcac cgcccacgcg
    70981 ccgtggcgca tcgcatctgc cgcctgagca ggcgtgttgt aacgcccttc ggcaatcacc
    71041 cgacatccgg cgtcgctcaa cgttttcacc agcgccagat ccggctcttc tggcgtttca
    71101 ggcgtggtat agccagaaag cgtagtgcca ataatttcgg ctcccagctt ttggcatgcc
    71161 aggccgtctt ccggcgttga gcagtcggtc atcgccagta aaccgtgatg gtgaatacgt
    71221 gccagcagcg tttcaacagg caccggacgc gggcggtcgg tgccgtcaat ggcgataatg
    71281 tccgcgcccg cctgcgccag cgcatcaaca tcttcaatat aggccgtgat gcgtaccgga
    71341 gaatcctcca gatcgcgttt cacaattcca ataatcggca cgctcaccac cgcacgcgtg
    71401 gcttgcagat ttgccacacc ttcaatgcga atggcaaccg cgcccgcctg ttctgccgct
    71461 aatgccatgg cagcgacgat ttcgggttta tcgagcgggc tgtccggaac cggctggcag
    71521 gagacaatca ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag taacgacata
    71581 catcttccct tagcgaaagg cccggtacag agaccgggca acaggattaa cttttggttt
    71641 tgactaaatc gtttttggcg ctgccaaatg gcacggcacc gctaaatggt ttgccgtcga
    71701 tagcgtcatg agtacgcaat gcttccgggc gcagccaacg ctgaacgcgg gaaggcatat
    71761 ccagaccaat cagcaggatc accacaaatg tcagactgaa cgatagcgat gccagcgcag
    71821 tacccagatc cagacgttga gcgatcaacg cgccgatgat tggggccagt gcgccgccca
    71881 atgcgccaac gttgtaggta aagcccaggc ccgctgcacg ctggtcggta tcgaaataac
    71941 cgccaatcag tttcggtaag atcccggcga tcccttgtcc aagcatttgc tggaagaaca
    72001 gtaacagacc gagcacccag acgtttgcgc cgccaatcgc aaataccgga ataatcaaca
    72061 gctgcgaggc caacaggcta caaacgtagg ctttgcgggt tcccagccag tcaccgagga
    72121 agccgctgac acagcacccc accgcagccc caaagccgct aaagaacagc acattggcga
    72181 cagtatgcgg gttataagcc agatcggttt tcagatacgt tggcagcagc gcctgaatcg
    72241 gccatgagta gaggaaagca aacaacacga ccaccatcag cattacgccc gttggccagc
    72301 gtttacccgt actttgcacc ataaagctga taaagattgc ggcgcataac agcccaagaa
    72361 cagcgacgat cgcggcattt tgcaggttac cggcgaagca aaaccacagc gcagtagccg
    72421 ccgccagtgt cattacgata ttggcaatgc gatgttcacc acggtagaga atatctacca
    72481 tcgtgcgtac tggtgcttta cctgcatgct tctctttcca gtcttccgct tccgggatgt
    72541 ttttacgcag ccagagagca aagatgatag gcaaaatgcc gataaagaac agcgcacgcc
    72601 agccccagac cggaaccacc aggctataga cctgagcggc aacgacggcc cccacagaga
    72661 agcctgaaat cagaaaacca ctggctttgt tacgcagatg ttttggccag ctttcaatga
    72721 cataggtggc gctggaaccg tattcacccg ccatccccat gccgatgacc agacgcgcga
    72781 taaacatggt gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc gagaagagaa
    72841 cgatgctggt gaccattgcc agacgacgcc cgtagcggtc acccatagcg ccgagcatca
    72901 ggccgccgaa ccagcgagag ataaaggctg cggagatcag acttgccgcc tgcaccgtcg
    72961 tcagcccgaa ttcaccctgt acttcggtga gtaccagggc gattaaaacg aaatcaaaac
    73021 cgtcaagcag atatcccaac caggcagcgg aaaatgcgcg ccattgtgca cggttgagat
    73081 ggcgatacca cgggatattc tgggttgtag tactcattgt gagtctcccg cggtgggcga
    73141 tgcccacacg ctttggtata aaaattgtag ggtacagatg cgtttatttc ccctcacccg
    73201 gtaggggcga gcgaggggaa acaactcacc cgcgctcttg catcaactgc tgggccagcg
    73261 ccttcagttc tggcagatat ttttcatcta ccggtccaaa cggtttgcgg cacagcggca
    73321 cagaaacgac atccatataa tggaggacag ttttcaggcc gcggaatacg cccgttttga
    73381 tcagtaaatc aatgacttta ttgcattcag tttgcagttt ctgcgcggtc tggatatcgc
    73441 cttctttcag cgccttaacg atcccctgat agcgccagcc catgatgttg taggtactac
    73501 cgataccacc atcagcgccc gccagcagac cagaggcgaa gatttcgtcg taaccgttat
    73561 agagcacaag atcaggatgt tcacgacgga tctgctccat ctgatagaga tcgccagagg
    73621 tctgtttcag cgcacctacg ccaggcaatg taacaagtgt gttgatctga tccagggtca
    73681 gttttacccc actcagggct ggaatgttgt acaccaccat cggcaaacca tccgccgaat
    73741 caataattgc ccgatagtga tcgcagtgtt cttcaaagct gaaaggatag tagaacggcg
    73801 tgacggcgga gacggcatcg aagccataac gtttagccga tgccgcaagt tgttggcttt
    73861 cggcggtgct gacgcaaccg acgtgggcga tgagtttaat cttacctttc gcctcttcgg
    73921 cgacgatttc cagtacctgt tcacgctcgg aaaggctttg tacaaaggcc tcgccggtcg
    73981 aaccacccac gtataaaccg tcgatgccct gctgaatatt gaactgaacc aggcgacgca
    74041 gactcgcttt atccagtgct tgttgttggt caaaaggagt caggagtgca gccattacgc
    74101 cacgtaaatt cgttgccata aatacctctg aagtgatgct tgtctgataa acgatatacc
    74161 tttatacctg ttataccaga tcgattaagc aacaccccat acagaaagct tataatgcga
    74221 tctgcttcac taaagtggca ttatttcttt ttgttggtgg tctgaccgaa agcgtgccag
    74281 gtagcagaga cgctgttgag atgcgattgc aacgcacgat cggcttcgtc aggatcatga
    74341 cggcggatcg catcaacgat cgcaatatgc tgttgataac taacgttgtt atgttcgtgc
    74401 agtgcctgat cggtaaccgt tgggcgtgcg gcaataagcc agtcgagcag ggcaacgtgg
    74461 atcgccatga agattgggtt accggggatc tccgccagca cgcggtggaa atcaacgtct
    74521 gaacgaatga atgccgcgtt gttatccagc gactgactgt tgatttccag tgcttttgcc
    74581 agcaaatcga tttgctcatc ggtggcatgt tcagccgcat agcgcaccag actggattca
    74641 aagaacagac gtaattgttc gaaatgggca atcccaccgg gatgagaaag gaaatctttc
    74701 gccatgccgg aaagctcacc gatgatagtg tccgcagaag gacgcgagac gcgagcgcgt
    74761 tcgccgttgt ttatttgcac cagacctttg cgttttaacg ctgccagcgc ttcacgcacc
    74821 gaaggacgcc cgacgttaaa gaacgccatc agttcgcgtt cagacggtaa ttgttcacct
    74881 tcgccaaatt cacgacggcg gatcatctgt tccagctctt cttccaccat ttcggagagt
    74941 tttttacgcg ccagcgggcg gctacgcaag ttgcgaccaa ttgcaggtga agaatcttcg
    75001 gtttgcgaat caaatgcgtt cataaggccc attctgtaag gtcagtgtga ttaacatcat
    75061 cagtgacatc ctatcacagg attgagagta ggggaaaatg gcaggttttt ctctttgtgc
    75121 ctcatcatta ccataattaa cggaataatt aactattgcg aaaaattaat gtaacgcaga
    75181 taaaaacatc ccgtttgaat tatttataag actattcacg agcattatga atattatgaa
    75241 tgcgttcttg caaaataatc ataagcgcat attttttaat gaaaaattat ctcacctaca
    75301 attaaaaaca cgacatccgc accataaata gacttgcaaa aaatataaca tcattgtttt
    75361 caatctgccg tttatgggat tgaccgtttt cttttgacac ggagttcaac aatgttcggc
    75421 ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa aaactacaaa
    75481 cctcaggtgg tgctggctgc cgcaggtatc ttcctgatga tgtgcggtgt ctggttaggg
    75541 ttcggtggtg tactcgagcc cgccaaaagc agcggctact tgatcgtcga tatttataat
    75601 gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctatcgat tatggcggtg
    75661 ggcggtatgc ccgctacatg gagcgcatag gggccagtcg cgcgatggtg agcttgttaa
    75721 gccgcccgtt aaaactcatt cgctcaccgt atattattct gtcggcaact tacgtcatcg
    75781 gtcaaatcat ggcgcagttt attaccagcg cctccggtct gggtatgttg ctgatggtca
    75841 ccttatttcc gacactggtg agtctgggag taagtcgtct ctctgcggtg gcggttatcg
    75901 caaccacaat gtccattgag tggggaattc tggaaacgaa ctccattttt gcagcccagg
    75961 tcgcgggaat gaaaattgcc acttacttct tccactacca gcttccggtc gcctcttgcg
    76021 tcattatctc ggtggcgatc tcccactttt tcgtgcaacg cgcttttgac aaaaaagata
    76081 aaaatatcaa tcacgaacaa gcagagcaaa aagctctcga taatgtcccg ccgctctatt
    76141 acgccatttt acctgtgatg ccgttaatcc tgatgctcgg ctcgctgttc ctcgcccacg
    76201 tcgggctgat gcagtcagaa ctgcatctgg tggtggtgat gttactgagt ttgacggtga
    76261 cgatgtttgt tgagttcttc cgcaagcata acttgcgcga aacaatggac gatgtgcagg
    76321 cgttttttga cggcatgggt acgcagtttg ccaacgtggt aacgctggtg gtcgcgggtg
    76381 aaatatttgc gaaaggctta acgacgattg gcactgtcga tgcggttatc aggggtgcgg
    76441 agcattctgg tctgggcggt attggcgtga tgattattat ggcgctggtc attgccattt
    76501 gtgccattgt gatgggctct ggcaatgcgc cgtttatgtc atttgccagt cttattccga
    76561 atatcgcagc cggactacat gtaccagcgg ttgtaatgat tatgccgatg cattttgcca
    76621 cgacgctagc gcgcgcggtt tcgccgatta ctgcggtggt ggtcgttacg tcaggaattg
    76681 caggcgtttc gccttttgcg gtggtgaagc gaacagcgat ccccatggca gtcggtttcg
    76741 tggtgaatat gattgccaca atcacgctat tttattaagc cattaaaaga caaaacaggc
    76801 caccagggcc tgtttttgta ttacttcaca acgcgtaatg ccggtcgacc accgcgtggt
    76861 ggctgcggag gttcatcgtc aggatgagtg tcatcatcgt gatctggctt gtcgccatca
    76921 ataaccgaca taacggtttc gttgtctgcc gatgcctctt catcattcat gatgctggta
    76981 tcttcatcgt aggcagcttc aggctcaaac attgtgcctg cgccattttc acgggcgtag
    77041 atagccagca cggcagccag cggcacagaa acctgacgcg gaatgccgcc aaagcgcgcg
    77101 ttaaagcgca cctcatcatt cgccagttcc agattgccga cagcacgcgg cgcaatgttg
    77161 agtacgattt gcccgtcacg cgcatattcc ataggaacct gcacgccagg gagcgtcaca
    77221 tccaccacta ggtgcggcgt gagctggtta tccagcaacc actcatagaa tgcacgcagc
    77281 agatagggac gacgtggtgt tagctgtgac aaatccatac agattaactc cggcccagac
    77341 gcatttcacg ttctgcttca gttaaagaag caaggaaaga gtcacgctca aagacgcggg
    77401 tcatatagcc tttcagctct ttcgcacccg ggccgctgaa ctcgatgccc agttgcggca
    77461 gacgccacag cagcggagca agatagcaat cgaccaggct gaactcatcg ctcaggaagt
    77521 acggcttctg accgaagacc ggcgcaatcg ccagcagttc ttcgcgcagt tgcttacgtg
    77581 cggcatctgc ttcagaagct gaaccgttga tgatggtgtt catcagcgtg taccagtctt
    77641 tttcgatgcg atgcatgtac agacggcttt caccgcgagc taccgggtaa acaggcatca
    77701 gtggcggatg cgggaaacgc tcatccagat attccataat gatgcgagat tcccacaggg
    77761 tcagctcacg atccaccagg gtcggaacgc tctgattcgg gttgaggtca atcagatcct
    77821 gaggcggatt gtccttttcc acgtgttcga tctcgaaact tacacctttc tcagccagca
    77881 caatgcggac ctgatggcta tagatgtcag taggaccgga aaacagcgtc attaccgaac
    77941 gtttgttggc agcgacagcc atgaaaacct ccaggtatag tcagaatttt tactgctacc
    78001 agccaccagg tggccagtca gaagttgtgt tacccagtaa ggaacgactc tctttgttcg
    78061 aaaatcaaac aaaaaatgag caatacccga catttgggca gaaaattgga tgatagttta
    78121 ccagattttg cgacctttgt ggtgagtcga tgccggaaat ggggaaaaag agatgcgctt
    78181 tagtctgaaa tagttgactt agtcccttat tggcgatgtg gtttttgttt tacctgtctg
    78241 tcaggtggca gcaaaaagca actttccagt ttttacgctg attcagattt tagctataaa
    78301 aaaacccgcc gaagcgggtt ttttcgaaaa ttgttttctg ccggagcaga agccaattaa
    78361 cgtttggaga actgcggacg acgacgtgct ttacgcagac cgactttctt acgttcaacc
    78421 tgacgagcgt cacgagtaac gaagccagct ttacgcagtt cagaacgcag ggactcgtcg
    78481 tattccatca gagcgcgggt gataccgtga cggatcgcac cagcctgacc agagatacca
    78541 ccacctttaa cggtgatgta caggtccagt ttctcaacca tgtcgaccag ttccagcggc
    78601 tgacgaacta ccatgcgggc agtttcacga ccgaagtact gttccagaga acgttggttg
    78661 attacgattt taccgttgcc cggtttgatg aaaacgcgag ctgcggaact tttgcggcga
    78721 ccagtgccgt agtattgatt ttcagccatt gcctataatc ccgattagat gtcaagaact
    78781 tgcggttgct gtgccgcgtg gttgtgctcg ttacccgcgt aaactttcag tttacggaac
    78841 atagcacgac ccagcgggcc ttttggcaac atgcctttaa ccgcgatttc aatcacacgc
    78901 tcaggacggc gagcaatcat ctcttcaaag gtcgcttgtt tgataccacc gatgtggccg
    78961 gtgtggtgat agtacacttt gtcagtacac ttgttgccgg ttacagcaac tttgtcagcg
    79021 ttcagaacga tgatgtaatc accggtatct acgtgcggag tgtattccgc tttgtgctta
    79081 ccgcgcaggc gacgagccag ttcagtagcc agacggccca gagttttacc ggtcgcgtca
    79141 acaacatacc agtcgcgttt tacggtttct ggtttagctg taaaagtttt cattaaaagc
    79201 ttacccaata aatagttaca cgttggtgaa cacccaaacg tcttcaattg ttgaggttca
    79261 cacgacaaag tccggcaaac ctaccccttc gaatagccta tgccagcaca caaaaagttt
    79321 tgggaaaaaa ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga
    79381 tcgacccctt tttgtgattt gtgacaggtt ttaacccgcc aaatgctcgc gcttcagata
    79441 ctcttcgctt tgcatctctt gcagacgtga caggcaacgc tggaactcaa acttcagccg
    79501 atcgccctga taaatttcat acagcggcac ttctgcactc accactaatt tgacatggcg
    79561 ctcgtaaaac tcatccacca gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt
    79621 cataactggt acatcaaaca acatgaccgt atgaaagagg cgtgagagcg caatatagtc
    79681 atgctggctg cgggcatcga cacacaaggt ggtaaaagag acagcaagcg tctgattctc
    79741 agcgcccatt gtcgccagcg gtcgatggtt gatttctaac gtcggtgaat tttcgcgttt
    79801 cgctcccgcc agcgccaacc atagtttatc catttgtgcc cgggtttcat cgtggagtgg
    79861 cgaaagccac agatgcgcct gagtgagtgt acgcagtcga taatcaacac cagcgtccac
    79921 gttcattaca tcacaatgct gtttaatggc atcgattgca ggcagaaaac gcgcacgttg
    79981 caagccattt cgataaagtt cgtccggcgg aatatttgac gttgctacca gggtaatacc
    80041 gcgggcgaac agggctttca tcagaccgcc aagtagcatg gcgtcggtaa tatcagaaac
    80101 aaaaaattcg tcaaaacaga gcacgtcagt ttcggcttta aagcgatcgg caataatttc
    80161 cagcggatcg gtctgcccct gtaaggcagt tagctcttcg tgcacccgca gcataaaacg
    80221 gtgaaagtgc aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag
    80281 ccaggttttc ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt
    80341 tgtgtcttcg cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg
    80401 ggctggtggc ctgctattga tgagttcttg ataaataatt tccaggcggc tgacggcctc
    80461 tttttgaacg tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt
    80521 tggggtaacg ctttgcatga tcttattatt attccttgaa taatcggtgc gccgttgttc
    80581 acggttgacg aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct
    80641 gtggaattaa cggttatagt ggcataatca gccgtaggca tggagcctga agccaacacc
    80701 ctacggaaac aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt
    80761 agtcgtcggc atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca
    80821 gcaacaggcg ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga
    80881 agagctggtt agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta
    80941 tcgccagctg tatcagcaca tggcgaaaag ctccagcagc ctgctgccgg aactgtctgc
    81001 tgaagcaaac ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc
    81061 ggtgcagatg cctcgcgact attctgaagg tgcatccggc ctgctgcgta ctggcgcgaa
    81121 gcgcgactaa tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga
    81181 ctatcattta atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt
    81241 tttgaatctc ttttcttatc attcaggtac gagagcagga ataatgaaaa aacaaaccca
    81301 gctgttgagt gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt
    81361 cgcgtcgatt ccaggccagg ttgccgatca ggcccctctc cccagtctgg ctccaatgct
    81421 ggaaaaagtg cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca
    81481 gaaaatcccg gaagaattca aaaagttttt tggtgatgat ttaccggatc aacctgcaca
    81541 acccttcgaa ggtttaggtt ccggtgtcat catcaacgcc agtaaaggct atgtgctgac
    81601 caacaaccat gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga
    81661 gtttgatgca aaactgattg gtagcgatga ccagagcgat atcgccctgt tacaaattca
    81721 aaacccgagc aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga
    81781 ttttgccgta gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt
    81841 ttccgcatta ggccgcagcg ggttgaatct tgaaggtctg gaaaacttta tccagacaga
    81901 tgcttccatt aaccgcggta actccggcgg tgcactatta aaccttaacg gtgagttaat
    81961 tggcatcaac actgcaatcc ttgcgcctgg cggcgggagc gtcgggattg gatttgccat
    82021 ccccagtaat atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg
    82081 cggtttgtta ggcatcaaag gcaccgagat gagtgccgat atcgccaaag ccttcaacct
    82141 tgacgtgcag cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcaaaagc
    82201 gggcgtcaaa gcgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc
    82261 tgagttgcgc tctcgtatcg cgactacaga gccgggcacg aaagtgaagc ttggcctgct
    82321 gcgtaacggc aaaccactgg aagtagaagt gacgcttgat accagcacct cttcgtcggc
    82381 cagcgctgaa atgatcacgc cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa
    82441 agattgcggc aaaggtatta agatcgatga agttgtcaaa ggaagcccag ctgctcaggc
    82501 aggcttgcaa aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc
    82561 tgaaatgcgt aaagtgctgg cggcaaaacc ggccatcatc gccctgcaaa ttgtacgcgg
    82621 caatgaaagc atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggcttacgtg
    82681 tgatgtccgg ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc
    82741 atgtttgtga agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg
    82801 gttgccatgc cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc
    82861 gatgagacgc ctgccagcta taatctggcg gttcgccgcg ccgcgccagc ggtggttaac
    82921 gtttacaacc gtggtgtgaa caccaactct cacaaccagc ttgagatccg caccctggga
    82981 tccggtgtaa tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac
    83041 gccgatcaga tcatcgtcgc cttacaggat ggacgtgtat ttgaagcatt gctggtggga
    83101 tctgactctc taaccgatct ggcggtactt aaaattaatg ccactggcgg tttacctacc
    83161 attccaatta atgcacgtcg cgtaccgcac attggcgacg tagtactggc gatcggtaac
    83221 ccgtacaacc tcgggcagac cattacccaa gggattatta gtgccacggg gcgaatcggt
    83281 ctgaacccta ccgggcggca aaacttccta caaaccgatg cttccattaa ccacggtaac
    83341 tctggcggcg cgctggtgaa ctcgctggga gaactgatgg gcattaacac gctgtcgttt
    83401 gataagagta acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta
    83461 gcaaccaaaa ttatggataa gctgatccgc gatgggcgcg tgatccgcgg ctacattggc
    83521 attggtggac gtgagatcgc accactgcac gcgcagagcg gtggtataga tcaactgcaa
    83581 gggatcgtgg ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc
    83641 aacgatctga ttatttcggt ggataacaaa ccggccatct ctgctctgga gacgatggat
    83701 caggtggcag aaattcgccc tggttcggtg atcccggttg tagtgatgcg tgatgataag
    83761 cagttaacgc tgcaggtcac cattcaggaa tatccggcaa ccaattaagt cgagtgctca
    83821 aaacaaaaaa ccggagtctg tgctccggtt ttttattatt cgctaatcaa ttacttatta
    83881 acgaactctt cgcccagggc gatatctttc ttgagcgtat ccagcatacc ttccagcgcg
    83941 ttctgttcaa atgcgctcag ggtaccgata gatttacgct cttccacgcc gtttttaccc
    84001 agcagcagcg gttgagagaa gaaacgggcg tactgaccgt cgccttcaac gtaggcacat
    84061 tcgacaacgc cttgttcgcc ctgcagtgca cgaaccagag acagaccaaa acgtgcagct
    84121 gcctggccca tagacagggt tgcagacccg ccaccggctt tcgcttcaac cacctcagta
    84181 cccgcgttct ggatacgttt ggtcagatca gccacttcct gctcggtaaa actaacgcca
    84241 ggaacctgtg acagcagcgg cagaatggta acaccagagt gaccgccaat aaccggcact
    84301 tcaacttcgc ctggctgttt gcctttcagt tccgcaacaa aggtgttgga acgaatgata
    84361 tccagcgtgg taacgccgaa cagtttgttt ttgtcataaa caccggcttt tttcagcact
    84421 tcagcagcaa ttgcaactgt ggtgttaacc gggttagtga taataccaat gcacgctttc
    84481 gggcaggttt tcgcaacttg ctgtaccagg tttttcacga tgccggcgtt aacgttaaac
    84541 aggtcggaac gatccatacc cggtttacgc gctacacctg cagagataag aacgacatct
    84601 gcgccttcca gcgccggagt cgcatcttca ccagaaaaac ctttgatttt cacagcagta
    84661 gggatatggc tcagatcgac agccacaccg ggagtcactg gagcgatatc atacagagag
    84721 agttctgaac ctgaaggcag ttgggttttt aacagtagtg caagcgcctg gccaataccg
    84781 ccagcagcgc cgaggactgc gactttcatc ctaaactcct tattatattg ataaactaag
    84841 atatgttgct ccgctgccgc gaccttaatc cacaaaattg ccgtttacaa tgaccacata
    84901 tcaagaatgt gtagtcacgc aagtttagcg tttatgcatt taattgccgt aatcaggaac
    84961 ctaacttacg taattaacag tcgcttatgc attagcgcaa cattccaaca ggtggtgaca
    85021 atatacccta ccgttcagcc aaaacaacat caatttgata acaattaatt tacttttaag
    85081 cagaatttgc atgccgtgac gcaggcatgt ttctcaataa cgaaatttga taaaatcccg
    85141 ctctttcata acattatttc agccttcttc agggttgact gtttgcataa aaattcatct
    85201 gtatgcacaa taatgttgta tcaaccacca tatcgggtga cttatgcgaa gctcggctaa
    85261 gcaagaagaa ctagttaaag catttaaagc attacttaaa gaagagaaat ttagctccca
    85321 gggcgaaatc gtcgccgcgt tgcaggagca aggctttgac aatattaatc agtctaaagt
    85381 ctcgcggatg ttgaccaagt ttggtgctgt acgtacacgc aatgccaaaa tggaaatggt
    85441 ttactgcctg ccagctgaac tgggtgtacc aaccacctcc agtccattga agaatctggt
    85501 gctggatatc gactacaacg atgcagttgt cgtgattcat accagccctg gcgcggcgca
    85561 gttaattgct cgcctgctgg actcactggg caaagcagaa ggtattctgg gcaccatcgc
    85621 tggcgatgac accatcttta ccacccctgc taacggtttc accgtcaaag acctgtacga
    85681 agcgatttta gagctgttcg accaggagct ttaatctctg ccccgtcgct tctggcggcg
    85741 gggaaaatgt tgcttatccc tctcaacccc ctgctttccc ctgcgattaa tttaacgaat
    85801 agtgcgtttt actgcgacat gtcattcaca taatgaatac atacggtaaa aaaagcacat
    85861 tatgcaaaat tcattatcta attgaaaata ctagaattaa aaataaatga ccgtgttttt
    85921 aattcttttt tgttattaaa attcacattt ttaacactta gtatcaactg aaacagttag
    85981 cgtggtatta attagttcaa taattagtgt atgcttgatt ttgtgatatg ggtcacaaaa
    86041 caaaggccca gctaaaagat tatgtcgagg taaaaatcat gaaaatcaaa accactgtag
    86101 ctgcattaag cgtactttct gttctctctt tcggtgcatt cgctgccgac tccattgatg
    86161 ctgcacaagc acaaaatcgt gaagcaatcg ggaccgtatc cgtaagtggt gtggcgtctt
    86221 cgccaatgga tatgcgtgaa atgctgaaca aaaaagcgga agagaaaggc gcaacggcct
    86281 accagattac tgaagctcgt agcggtgaca cctggcacgc tacggctgaa ctgtacaaat
    86341 aaaccctcat cgtcttgtcc gacgatattg cccccggttc gggggctttt tttgcgctaa
    86401 tgacgaacat taaaacgcaa atgcccttcc agctcttcct ctgcctcatc aaacagcaat
    86461 attaacgcgc caaaacggcg acgcgttttc tctcccagat gaacaaattc aatctcaagt
    86521 ggtagcggca ggacatcgtt cattaacaca tcccatagtg agtcgagatc gcgtacctta
    86581 tctttcgcca gaccaaaggt ttggctaaag tcacgataaa aatcctcctg actctcaatc
    86641 tcatcaaaat caaaggtata aatattcatc tattgccacc gtcacgtttc gcggcgagtt
    86701 ctgccgtgca tcgctaagta tagccatgaa aaaaccgaca cttttggcgt cggttttgac
    86761 ctaactatcg gtcaacgcat gttgatactt atggagcata cccgccagcc gatgaaccgg
    86821 ttccgtcacc tgcggtggcg cttgccagat gcgtagcttt tcctggtaga tttcaagttc
    86881 ttccagcaac tggccaaagt accgacgacg tttatcatcg ctacgggcag atatcacatg
    86941 gtctgctgtg cggcgcattt gtcggtgaaa cgccgataaa tcctcgttaa ccgggatcgg
    87001 cgcatcacgc aggcgctggt gcgcgataat catcgtcagc gccaggcgaa atttcggcaa
    87061 atcccctggg aacttattca tcagcaaaaa cagctgctga taaagtgccg ggaggtggtt
    87121 ctctttacga cgtgccacat tggtagtcat cgcggaaaca gcggcagaaa caaactgatt
    87181 aagcagtaca cgttcggtcc tgtcgcgcga tttatcccgc acaagcaaaa taacggtgaa
    87241 cgcgagcaca cagccgacga tttgccctaa tgcgctgtcg agaaactgac tgaaatggaa
    87301 agtcatcggg ttatccagca cgataatatt tatggtgctg gccagcgccc ccatcgagcc
    87361 cagtcgccgt tgctgtactt ctataccgag gaaaaatccc agcactgcca ggctaataca
    87421 cagcagcaac atgctctgtt gggtattagg gataatcacc aaaaagtaga gcagccctaa
    87481 cggcagcgcg gccagcgtcc cgtagataaa gtcgatcgcc accatgcgtg gattcggcaa
    87541 acgcattgcc agtgacgtca ctaccgcaat catcaccatt gcaccactgc cggaagtcca
    87601 gcccgtccac agccagaaaa gcgtgcccag aatgcaggaa agtgtggttc gccagaagtt
    87661 aaccatcgca tgatgacgtt cggctgactc tactttgact tccggttcgc cttgcaggat
    87721 ctcttcttcg gtggcgttga tttttgtgtt actgataacg ccgcgcttga gaagctgata
    87781 acgcgttgcc gccgcgaccc agctataaat ggtgacaggc gtttcccgtt ccccggtcca
    87841 ggcgataact ctccgcaggc gtttgagctg cttgtggacg tcctgcgcgg tttctaccgg
    87901 cgtgtcaaaa aattcgcgga aagtatcagt gatcaattcc gggcgcgtat tctgaataag
    87961 ataagtttcg caggattggg taatcagcgt cagcgatagc gtattgatcg cttttaaacg
    88021 tcgattggcc cgcgcccagc gggaagattc catattcagg ttgctgcgca tgccttgtag
    88081 cgccgtggtg cgtcgcacca ggtcgcccca ggctttatcg acaacttcac catcgccatg
    88141 cttgatacag agttgcatta attgatattg cgcgaccagc aaactttcca gctctcgatc
    88201 cacttcttgt ttgatcgatc gcggagaaaa gagcaaatcc gctataatcg cacacacaat
    88261 accgataacg atctcgctac aacgttcgac ggcaaactgc ggcgtaagca atggttccgg
    88321 ctgaatggtg atcacaatga tcagcgcggt ataaccggcc agcccccacg cgtacgagtt
    88381 ttctattcgt accagcgagg atatccaggt acaaaaaccg gcccagatac agcacaccag
    88441 aatcatcaat aatggtgcgc ggatcatcgc aatgatgatc accagtccgg caatacagcc
    88501 aataaatgtg ccgatgatgc gcaaaaagcc acgataacga atagcgccag aatacggttc
    88561 acctcccgca gcaaaggccg gaccggcggc aacaatcgcc gctgtcagta ccgcccagcg
    88621 tggcgtttcc agctggaagt gaaagccaac aaacagcgcc agtacaatgg cggtcgccag
    88681 ttttaccgca aagcgaatat gttggttagc aatggagaaa atacccatcg tgattaacca
    88741 aactcacgca ggcgatgggc cattttacgg aagaacgaat cctggctttc gtcgcgatct
    88801 tgtttgccag tgaccaccac tgtagcagtg gtgcccgcag gccagatgtt ctcttgctgg
    88861 ttgtcgagac gaatacgaac cggaacacgt tgcgcaagac gcacccattc aaggttagag
    88921 tctatagtcg ccatcccttt gtcgtcacgc gtgctgctgg cgttggtgac ccctgcggca
    88981 acactatcaa cagtcccttt cagcactttg ttactgccaa gcggcgtgat ctctgcacga
    89041 taccccggac gcaccccttc cagcttagtt tcttccatat aggccagtac atagaaggag
    89101 ttctgtttca ccagcgcaac cgccgttgat cctcgagtaa taaactcacc ggtatagacg
    89161 ttgaggttgg tcacccagcc atctgctggc gcgcggatca ccgtgcgttc aagatccagt
    89221 tttgccagat cgcgggtcgc ctgcgctttc gctaactgat gcagaaccgt ttgtagtacg
    89281 ttgttagcct agtcgatctc ttcgcgagac atcgcctgca caccgagacg gttacgacgt
    89341 ccggcctcct ggcgtttctc ctgtgccagt acctgataat aagcaacatc ggcttgcgct
    89401 tcctcaagcg ccttttgata gcgcggctgg tcgatggtga acagtatctg tccttttttc
    89461 accagctggt tatcatgaac attcacctgg gtaatgagtc cagaaacgtc cggcgcgatc
    89521 gcaatgacgt cagcgctaaa gcgcgcgtca cgcgtccagg gggattcggt gtaatagacc
    89581 caggcattaa aaattgcgat gaaggccaga atgactaata cgaccgtgat ggccgtacgg
    89641 gagaattttc ttattagtgt tttcacttca acctcaaacg aacagtcgcg atatcaaata
    89701 aaacaagcag caatagagcg cggtgttgaa caacgccgga tgccagacaa agtcgtagat
    89761 acctgttggc acaagtaccc ggcgcaccag ccagaaaatc gccagtgata aaag
//
LOCUS       DAJTNN010000002        80905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTNN010000002 DAJTNN010000000
VERSION     DAJTNN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 80905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.002"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            555..1757
                     /gene="proV"
                     /locus_tag="OO150_000095"
     CDS             555..1757
                     /gene="proV"
                     /locus_tag="OO150_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5514381.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1750..2814
                     /gene="proW"
                     /locus_tag="OO150_000096"
     CDS             1750..2814
                     /gene="proW"
                     /locus_tag="OO150_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5514382.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQVLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPSIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2872..3864
                     /gene="proX"
                     /locus_tag="OO150_000097"
     CDS             2872..3864
                     /gene="proX"
                     /locus_tag="OO150_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5514383.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTLLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKEPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAALK"
     gene            4156..5340
                     /locus_tag="OO150_000098"
     CDS             4156..5340
                     /locus_tag="OO150_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5514384.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRPRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5464..6201
                     /gene="ygaZ"
                     /locus_tag="OO150_000099"
     CDS             5464..6201
                     /gene="ygaZ"
                     /locus_tag="OO150_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5514385.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            6191..6526
                     /gene="ygaH"
                     /locus_tag="OO150_000100"
     CDS             6191..6526
                     /gene="ygaH"
                     /locus_tag="OO150_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5514386.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6617..7147
                     /gene="emrR"
                     /locus_tag="OO150_000101"
     CDS             6617..7147
                     /gene="emrR"
                     /locus_tag="OO150_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5514387.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            7274..8446
                     /gene="emrA"
                     /locus_tag="OO150_000102"
     CDS             7274..8446
                     /gene="emrA"
                     /locus_tag="OO150_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR5514388.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTVREISLAPVNKLIDDIVKANAG"
     gene            8463..10001
                     /gene="emrB"
                     /locus_tag="OO150_000103"
     CDS             8463..10001
                     /gene="emrB"
                     /locus_tag="OO150_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5514389.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWECGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(10065..10580)
                     /gene="luxS"
                     /locus_tag="OO150_000104"
     CDS             complement(10065..10580)
                     /gene="luxS"
                     /locus_tag="OO150_000104"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5514390.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10730..12286)
                     /gene="gshA"
                     /locus_tag="OO150_000105"
     CDS             complement(10730..12286)
                     /gene="gshA"
                     /locus_tag="OO150_000105"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5514391.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTQATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGKAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(12359..12787)
                     /locus_tag="OO150_000106"
     CDS             complement(12359..12787)
                     /locus_tag="OO150_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5514392.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12784..13350)
                     /gene="yqaB"
                     /locus_tag="OO150_000107"
     CDS             complement(12784..13350)
                     /gene="yqaB"
                     /locus_tag="OO150_000107"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5514393.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLAPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13498..13574)
                     /locus_tag="OO150_000108"
     tRNA            complement(13498..13574)
                     /locus_tag="OO150_000108"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13538..13540),aa:Arg,seq:acg)
     gene            complement(13773..13849)
                     /locus_tag="OO150_000109"
     tRNA            complement(13773..13849)
                     /locus_tag="OO150_000109"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13813..13815),aa:Arg,seq:acg)
     gene            complement(14047..14123)
                     /locus_tag="OO150_000110"
     tRNA            complement(14047..14123)
                     /locus_tag="OO150_000110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14087..14089),aa:Arg,seq:acg)
     gene            complement(14127..14219)
                     /locus_tag="OO150_000111"
     tRNA            complement(14127..14219)
                     /locus_tag="OO150_000111"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14183..14185),aa:Ser,seq:gct)
     gene            complement(14535..14720)
                     /gene="csrA"
                     /locus_tag="OO150_000112"
     CDS             complement(14535..14720)
                     /gene="csrA"
                     /locus_tag="OO150_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5514394.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14955..17585)
                     /gene="alaS"
                     /locus_tag="OO150_000113"
     CDS             complement(14955..17585)
                     /gene="alaS"
                     /locus_tag="OO150_000113"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5514395.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKDANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEVAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17713..18213)
                     /gene="recX"
                     /locus_tag="OO150_000114"
     CDS             complement(17713..18213)
                     /gene="recX"
                     /locus_tag="OO150_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5514396.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYDEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18456..19517)
                     /gene="recA"
                     /locus_tag="OO150_000115"
     CDS             complement(18456..19517)
                     /gene="recA"
                     /locus_tag="OO150_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5514397.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19597..20094)
                     /gene="pncC"
                     /locus_tag="OO150_000116"
     CDS             complement(19597..20094)
                     /gene="pncC"
                     /locus_tag="OO150_000116"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5514398.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(20239..21324)
                     /gene="mltB"
                     /locus_tag="OO150_000117"
     CDS             complement(20239..21324)
                     /gene="mltB"
                     /locus_tag="OO150_000117"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5514399.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21580..22143
                     /gene="srlA"
                     /locus_tag="OO150_000118"
                     /gene_synonym="gutA"
     CDS             21580..22143
                     /gene="srlA"
                     /locus_tag="OO150_000118"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR5514400.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSQGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLALSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            22140..23099
                     /gene="srlE"
                     /locus_tag="OO150_000119"
                     /gene_synonym="gutE"
     CDS             22140..23099
                     /gene="srlE"
                     /locus_tag="OO150_000119"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5514401.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            23110..23481
                     /gene="srlB"
                     /locus_tag="OO150_000120"
                     /gene_synonym="gutB"
     CDS             23110..23481
                     /gene="srlB"
                     /locus_tag="OO150_000120"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5514402.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23485..24264
                     /gene="srlD"
                     /locus_tag="OO150_000121"
     CDS             23485..24264
                     /gene="srlD"
                     /locus_tag="OO150_000121"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5514403.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24369..24727
                     /gene="gutM"
                     /locus_tag="OO150_000122"
                     /pseudo
     CDS             24369..24727
                     /gene="gutM"
                     /locus_tag="OO150_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            24794..25567
                     /gene="srlR"
                     /locus_tag="OO150_000123"
                     /gene_synonym="gutR"
     CDS             24794..25567
                     /gene="srlR"
                     /locus_tag="OO150_000123"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5514404.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25560..26525
                     /gene="gutQ"
                     /locus_tag="OO150_000124"
     CDS             25560..26525
                     /gene="gutQ"
                     /locus_tag="OO150_000124"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5514405.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26522..28036)
                     /gene="norR"
                     /locus_tag="OO150_000125"
     CDS             complement(26522..28036)
                     /gene="norR"
                     /locus_tag="OO150_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5514406.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            28223..29662
                     /gene="norV"
                     /locus_tag="OO150_000126"
     CDS             28223..29662
                     /gene="norV"
                     /locus_tag="OO150_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5514407.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCRVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            29659..30792
                     /gene="norW"
                     /locus_tag="OO150_000127"
                     /pseudo
     CDS             29659..30792
                     /gene="norW"
                     /locus_tag="OO150_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
     gene            complement(30920..33172)
                     /gene="hypF"
                     /locus_tag="OO150_000128"
     CDS             complement(30920..33172)
                     /gene="hypF"
                     /locus_tag="OO150_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5514408.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(33325..33852)
                     /gene="hydN"
                     /locus_tag="OO150_000129"
     CDS             complement(33325..33852)
                     /gene="hydN"
                     /locus_tag="OO150_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5514409.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(34001..35011)
                     /gene="ascG"
                     /locus_tag="OO150_000130"
     CDS             complement(34001..35011)
                     /gene="ascG"
                     /locus_tag="OO150_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5514410.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            35271..36728
                     /gene="ascF"
                     /locus_tag="OO150_000131"
     CDS             35271..36728
                     /gene="ascF"
                     /locus_tag="OO150_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR5514411.1"
                     /translation="MAKNYAALARSEIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTPRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYSIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            36737..38161
                     /gene="ascB"
                     /locus_tag="OO150_000132"
     CDS             36737..38161
                     /gene="ascB"
                     /locus_tag="OO150_000132"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR5514412.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(38320..38790)
                     /gene="hycI"
                     /locus_tag="OO150_000133"
     CDS             complement(38320..38790)
                     /gene="hycI"
                     /locus_tag="OO150_000133"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR5514413.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(38783..39193)
                     /gene="hycH"
                     /locus_tag="OO150_000134"
     CDS             complement(38783..39193)
                     /gene="hycH"
                     /locus_tag="OO150_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR5514414.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(39190..39957)
                     /gene="hycG"
                     /locus_tag="OO150_000135"
     CDS             complement(39190..39957)
                     /gene="hycG"
                     /locus_tag="OO150_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR5514415.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            complement(39957..40499)
                     /gene="hycF"
                     /locus_tag="OO150_000136"
     CDS             complement(39957..40499)
                     /gene="hycF"
                     /locus_tag="OO150_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR5514416.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(40509..42218)
                     /gene="hycE"
                     /locus_tag="OO150_000137"
     CDS             complement(40509..42218)
                     /gene="hycE"
                     /locus_tag="OO150_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5514417.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKEQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(42236..43159)
                     /gene="hycD"
                     /locus_tag="OO150_000138"
     CDS             complement(42236..43159)
                     /gene="hycD"
                     /locus_tag="OO150_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5514418.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITSRITWAGFGFAFLAFVSLLAA"
     gene            complement(43162..44988)
                     /gene="hycC"
                     /locus_tag="OO150_000139"
     CDS             complement(43162..44988)
                     /gene="hycC"
                     /locus_tag="OO150_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5514419.1"
                     /translation="MSAISLINSGVVWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILILSLLGGNAPLWWGIVLLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(44985..45596)
                     /gene="hycB"
                     /locus_tag="OO150_000140"
     CDS             complement(44985..45596)
                     /gene="hycB"
                     /locus_tag="OO150_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5514420.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNVITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(45721..46182)
                     /gene="hycA"
                     /locus_tag="OO150_000141"
     CDS             complement(45721..46182)
                     /gene="hycA"
                     /locus_tag="OO150_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5514421.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            46394..46744
                     /gene="hypA"
                     /locus_tag="OO150_000142"
     CDS             46394..46744
                     /gene="hypA"
                     /locus_tag="OO150_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5514422.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            46748..47620
                     /gene="hypB"
                     /locus_tag="OO150_000143"
     CDS             46748..47620
                     /gene="hypB"
                     /locus_tag="OO150_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5514423.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            47611..47883
                     /gene="hypC"
                     /locus_tag="OO150_000144"
     CDS             47611..47883
                     /gene="hypC"
                     /locus_tag="OO150_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5514424.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            47883..49004
                     /gene="hypD"
                     /locus_tag="OO150_000145"
     CDS             47883..49004
                     /gene="hypD"
                     /locus_tag="OO150_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5514425.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            49001..50011
                     /gene="hypE"
                     /locus_tag="OO150_000146"
     CDS             49001..50011
                     /gene="hypE"
                     /locus_tag="OO150_000146"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5514426.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            50085..52163
                     /gene="flhA"
                     /locus_tag="OO150_000147"
                     /pseudo
     CDS             50085..52163
                     /gene="flhA"
                     /locus_tag="OO150_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(52200..52553)
                     /locus_tag="OO150_000148"
     CDS             complement(52200..52553)
                     /locus_tag="OO150_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5514427.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(52630..52764)
                     /locus_tag="OO150_000149"
     CDS             complement(52630..52764)
                     /locus_tag="OO150_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514428.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            52840..55401
                     /gene="mutS"
                     /locus_tag="OO150_000150"
     CDS             52840..55401
                     /gene="mutS"
                     /locus_tag="OO150_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5514429.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            55507..56163
                     /gene="pphB"
                     /locus_tag="OO150_000151"
     CDS             55507..56163
                     /gene="pphB"
                     /locus_tag="OO150_000151"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCR5514430.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMELDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(56214..56981)
                     /locus_tag="OO150_000152"
     CDS             complement(56214..56981)
                     /locus_tag="OO150_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5514431.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            57177..58085
                     /locus_tag="OO150_000153"
     CDS             57177..58085
                     /locus_tag="OO150_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5514432.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRVGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGDAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            58082..59344
                     /locus_tag="OO150_000154"
     CDS             58082..59344
                     /locus_tag="OO150_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5514433.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            59341..59979
                     /locus_tag="OO150_000155"
     CDS             59341..59979
                     /locus_tag="OO150_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5514434.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTVLSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            59984..60758
                     /locus_tag="OO150_000156"
                     /pseudo
     CDS             59984..60758
                     /locus_tag="OO150_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
     gene            60847..62211
                     /locus_tag="OO150_000157"
     CDS             60847..62211
                     /locus_tag="OO150_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR5514435.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(62305..63297)
                     /gene="rpoS"
                     /locus_tag="OO150_000158"
     CDS             complement(62305..63297)
                     /gene="rpoS"
                     /locus_tag="OO150_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5514436.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(63360..64451)
                     /gene="nlpD"
                     /locus_tag="OO150_000159"
     CDS             complement(63360..64451)
                     /gene="nlpD"
                     /locus_tag="OO150_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5514437.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(64591..65217)
                     /gene="pcm"
                     /locus_tag="OO150_000160"
     CDS             complement(64591..65217)
                     /gene="pcm"
                     /locus_tag="OO150_000160"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5514438.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(65211..65972)
                     /gene="surE"
                     /locus_tag="OO150_000161"
     CDS             complement(65211..65972)
                     /gene="surE"
                     /locus_tag="OO150_000161"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5514439.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(65953..67002)
                     /gene="truD"
                     /locus_tag="OO150_000162"
     CDS             complement(65953..67002)
                     /gene="truD"
                     /locus_tag="OO150_000162"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5514440.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     AAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQRKALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(66999..67478)
                     /gene="ispF"
                     /locus_tag="OO150_000163"
     CDS             complement(66999..67478)
                     /gene="ispF"
                     /locus_tag="OO150_000163"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5514441.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(67478..68188)
                     /gene="ispD"
                     /locus_tag="OO150_000164"
     CDS             complement(67478..68188)
                     /gene="ispD"
                     /locus_tag="OO150_000164"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5514442.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(68207..68518)
                     /gene="ftsB"
                     /locus_tag="OO150_000165"
     CDS             complement(68207..68518)
                     /gene="ftsB"
                     /locus_tag="OO150_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5514443.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(68712..69035)
                     /locus_tag="OO150_000166"
     CDS             complement(68712..69035)
                     /locus_tag="OO150_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5514444.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(69085..69690)
                     /gene="cysC"
                     /locus_tag="OO150_000167"
     CDS             complement(69085..69690)
                     /gene="cysC"
                     /locus_tag="OO150_000167"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5514445.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(69690..71117)
                     /gene="cysN"
                     /locus_tag="OO150_000168"
     CDS             complement(69690..71117)
                     /gene="cysN"
                     /locus_tag="OO150_000168"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5514446.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     PSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(71119..72027)
                     /gene="cysD"
                     /locus_tag="OO150_000169"
     CDS             complement(71119..72027)
                     /gene="cysD"
                     /locus_tag="OO150_000169"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5514447.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            72279..73316
                     /gene="iap"
                     /locus_tag="OO150_000170"
     CDS             72279..73316
                     /gene="iap"
                     /locus_tag="OO150_000170"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5514448.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYISQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTTEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(73523..73816)
                     /gene="cas2"
                     /locus_tag="OO150_000171"
     CDS             complement(73523..73816)
                     /gene="cas2"
                     /locus_tag="OO150_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR5514449.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(73816..74736)
                     /gene="cas1e"
                     /locus_tag="OO150_000172"
     CDS             complement(73816..74736)
                     /gene="cas1e"
                     /locus_tag="OO150_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCR5514450.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLQVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(74733..75383)
                     /gene="cas6e"
                     /locus_tag="OO150_000173"
     CDS             complement(74733..75383)
                     /gene="cas6e"
                     /locus_tag="OO150_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR5514451.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDISVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(75365..76111)
                     /gene="cas5e"
                     /locus_tag="OO150_000174"
     CDS             complement(75365..76111)
                     /gene="cas5e"
                     /locus_tag="OO150_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCR5514452.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSLSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(76122..77200)
                     /gene="cas7e"
                     /locus_tag="OO150_000175"
                     /pseudo
     CDS             complement(76122..77200)
                     /gene="cas7e"
                     /locus_tag="OO150_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            complement(77215..77769)
                     /gene="casB"
                     /locus_tag="OO150_000176"
     CDS             complement(77215..77769)
                     /gene="casB"
                     /locus_tag="OO150_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCR5514453.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLEEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALGAGRWALEYYQAGDADNEQN"
     gene            complement(77766..79328)
                     /gene="casA"
                     /locus_tag="OO150_000177"
     CDS             complement(77766..79328)
                     /gene="casA"
                     /locus_tag="OO150_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCR5514454.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRSWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATFIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPDELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            complement(79426..80898)
                     /gene="cas3"
                     /locus_tag="OO150_000178"
                     /pseudo
     CDS             complement(79426..80898)
                     /gene="cas3"
                     /locus_tag="OO150_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
BASE COUNT        19081 a        21967 c        20685 g        19172 t
ORIGIN      
        1 atacttttgc gggtcaacat aagaggatga gtccttaata aaacaggtcc tcagagcata
       61 catattctgc ggagcgtgcg gcaacaacag gacgaacagg ggcggttttc aatttgcagc
      121 cgtcaggctg ccgtggttca ggctcctctt atgtgatggg gaaagcggtt gaaacagaag
      181 atgaagactg gaatttctga gggtgttatt ttcaaaaata tcactacccg cagcagggaa
      241 ataattcccg ccaaatagct ttttatcacg caaatattct gtagtgatct acactgagac
      301 tctgttgcat tattcgcctg aaactacaat attcaggcgt tttttcgcta tctatgacaa
      361 aaaatatcaa cttttctcga tttgctctca gcccttatat cacgggaaat tccggcgatt
      421 tgctcgcatc aatattcatg ccacatttgc cctcaggggt tgcctcagat tctcagtatg
      481 ttagggtaga aaaaagtgac tatttccatt gggtaatata tcgacataaa caaataaagg
      541 aatctttcta ttgcatggca attaaattag aaattaaaaa tctttataaa atatttggcg
      601 agcatccaca gcgagcgttc aaatatatcg aacaaggact ttcgaaagaa caaattctgg
      661 aaaaaactgg gctatcgctt ggcgtaaaag acgccagtct ggccattgaa gaaggcgaga
      721 tatttgtcat catgggatta tccggctcgg gtaaatccac aatggtacgc cttctcaatc
      781 gcctgattga acccacccgc gggcaagtgc tgattgatgg tgtggatatt gccaaaatat
      841 ccgatgccga actccgtgag gtgcgcagaa aaaagattgc gatggtcttc cagtcctttg
      901 ccttaatgcc gcatatgacc gtgctggaca atactgcgtt cggtatggaa ttggccggaa
      961 ttaatgccga agaacgccgg gaaaaagccc ttgatgcact gcgtcaggtc gggctggaaa
     1021 attatgccca cagctacccg gatgaactct ctggcgggat gcgtcaacgt gtgggattag
     1081 cccgcgcgtt agcgattaat ccggatatat tattaatgga cgaagccttc tcggcgctcg
     1141 atccattaat tcgcaccgag atgcaggatg agctggtaaa attacaggcg aaacatcagc
     1201 gcaccattgt ctttatttcc cacgatctcg atgaagctat gcgtattggc gaccgaattg
     1261 ccattatgca aaatggtgaa gtggtacagg tcggcacacc ggatgaaatt ctcaataatc
     1321 cggcgaatga ttatgtccgt accttcttcc gtggcgttga tattagtcag gtattcagtg
     1381 cgaaagatat tgcccgccgg acaccgaatg gcttaattcg taaaacccct ggcttcggcc
     1441 cacgttcggc actgaaatta ttgcaggatg aagatcgcga atatggctac gttatcgaac
     1501 gcggtaataa gtttgtcggc gcagtttcca tcgattcgct taaaaccgcg ttaacgcagc
     1561 agcaaggtct tgatgcggcg ctgattgatg cgccgttagc agtcgatgca caaacgcctc
     1621 ttagcgagtt gctctctcat gtcggacagg caccctgtgc ggtacccgtg gtcgacgagg
     1681 accaacagta tgtcggcatc atttcgaaag gaatgctgct gcgcgcttta gatcgtgagg
     1741 gggtaaataa tggctgatca aaataatccg tgggatacca cgccagcggc ggacagtgcc
     1801 gtgcaatccg cagacgcctg gggtacaccg gcgactgcac cgactgacgg cggtggtgct
     1861 gactggctga ccagtacgcc tgcgccaaac gtcgagcatt ttaatattct cgatccgttc
     1921 cataaaacgc tgatcccgct cgacagttgg gtcactgaag ggatcgactg ggttgttacc
     1981 catttccgtc ccgtcttcca gggcgtgcgc gttccggttg attatatcct caacggtttc
     2041 cagcaagtgc tgctgggtat gcccgcaccg gtggcgatta tcgttttcgc tctcatcgcc
     2101 tggcagattt ccggggtcgg aatgggcgtg gcgacgctgg tttcgctgat tgccatcggc
     2161 gcaatcggtg cctggtcgca ggccatggtt accctggcgc tggtgttaac cgccctgctg
     2221 ttctgtatcg tcattggttt gccgctgggg atctggctgg cgagaagtcc gcgagcggcg
     2281 aaaattattc gtccactgct tgatgccatg cagaccacgc cagcgtttgt ttatctggtg
     2341 ccaatcgtca tgctatttgg tatcggtaac gtgccgggcg tggtggtgac gatcatcttt
     2401 gctctgccgc cgattatccg tctgaccatt ctggggatta accaggttcc ggcggatctg
     2461 attgaagcct cgcgctcatt cggtgccagc ccgcgccaga tgctgttcaa agttcagtta
     2521 ccgctggcga tgccgagcat tatggcgggc gttaaccaga cgctgatgct ggccctttct
     2581 atggtggtca ttgcctcgat gattgccgtc ggcgggctgg gtcagatggt acttcgcggt
     2641 atcggtcgtc tggatatggg gcttgccacc gttggcggcg tcgggattgt gatcctcgcc
     2701 attatcctcg atcgcctgac gcaggccgtt gggcgcgact cacgcagtcg cggcaaccgt
     2761 cgctggtaca ccactggccc tgttggtctg ctgacccgcc cattcattaa gtaactctgc
     2821 acttgcccgg tgacgccggg cattatcacc ctgccaaaaa aaggaataac aatgcgacat
     2881 agcgtacttt ttgcgacagc gtttgccacg cttatctcta cacaaacttt tgctgccgat
     2941 ctgccgggca aaggtattac tgttaatcca gttcaaagca ccatcaccga agaaaccttc
     3001 cagacgctgc tggtcagccg tgcgctggag aaattaggct ataccgtcaa caaacccagc
     3061 gaagtagatt acaacgttgg ctacaccttg cttgcttccg gcgatgcaac cttcaccgcc
     3121 gtgaactgga cgccactgca tgacaacatg tacgaagctg ccggtggcga taagaaattt
     3181 tatcgtgaag gggtatttgt taacggcgcg gcacagggtt acctgatcga taagaaaacc
     3241 gccgaccagt acaaaatcac caacatcgca caactgaaag agccgaagat cgccaaactg
     3301 ttcgatacca acggcgacgg taaagcggat ttaaccggtt gtaatcctgg ctggggctgc
     3361 gaaggtgcga tcaaccacca gcttgccgcg tatgaactga ccaacaccgt gacgcataat
     3421 caggggaact acgcagcgat gatggccgac accatcagtc gctacaaaga gggcaaaccg
     3481 gtgttttatt acacctggac gccgtactgg gtgagtaatg agctgaagcc agggaaagat
     3541 gtggtctggt tgcaggtgcc gttctccgca ctgccgggcg ataaaaatgc cgataccaaa
     3601 ctgccgaatg gcgcgaatta cggcttcccg gtcagcacca tgcatatcgt tgccaacaaa
     3661 gcctggaccg agaaaaatcc ggcggcagcc aaactgtttg ccattatgca gttgccagtg
     3721 gccgatatta acgcccagaa cgccattatg catgacggca aagcctcaga aggcgatatt
     3781 cagggccacg ttgatggctg gatcaaagcc caccagcagc agttcgatgg ctgggtgaat
     3841 gaggcgctgg cagcgctgaa gtaattttta ttcaggcgga taaggcgttt ttgccttatc
     3901 cgccgctctg tacacaatgc ctgatgcgac gctgacgcgt cttatcatgc ctacaaaata
     3961 cactcattcc gtatggcgga taaggcgttt tcgccgcatc cgccgttctg tgcacaatgc
     4021 ctgatgcgac gctggcgcgt cttatcatgc ctacaaacca catcgcacat ttcaacaagc
     4081 tatctttcat cccatattca tcaacatccg ctattattga tttccagctt aatcatcacc
     4141 tgatgaacaa aaataatgac taaacctaat catgagctta gcccggcgct gatcgtgctg
     4201 atgtctatcg ccaccggtct ggcggtagcc agtaactatt acgcccagcc attgctcgac
     4261 accatcgcgc gtaacttttc cctttccgcc agttcggcag gctttattgt taccgccgcg
     4321 caattgggct atgcagcagg tctgctgttt cttgttcccc tcggtgatat gtttgaacgc
     4381 cgccgcctga ttgtctcgat gaccttactg gcggcaggcg gcatgttaat cactgccagc
     4441 agtcagtcgc tggcgatgat gatcctcggt acggcattaa ccggtttatt ctctgtcgtg
     4501 gcacaaattc tggttccgct ggcagcgacg ctggcttcac cggacaaacg cggcaaagtg
     4561 gttggcacta ttatgagcgg tctgctgttg gggatcttac tcgcacggac cgttgccgga
     4621 ttgctggcga atctcggcgg ctggcgcacc gtcttttggg ttgcctcggt gttaatggca
     4681 ctgatggcgc tggcattatg gcgtggtctg ccacaaatga aatcagaaac ccacctcaac
     4741 tacccacagt tgttaggttc cgttttcagc atgtttatca gcgataagat cctgcgcccc
     4801 cgcgcattgc tgggctgcct gacctttgcc aacttcagca ttctctggac ctcaatggcc
     4861 tttttgcttg ccgctccacc ttttaactac agcgatggtg tcattggtct gtttggactt
     4921 gcgggagctg ccggagcgtt gggcgctcgt ccggcgggcg gttttgccga taagggtaaa
     4981 tcgcaccaca ccacaacttt cggtctgctg ctgctattac tttcatggct ggcgatctgg
     5041 tttggtcaca cttccgtact ggcgctgatt atcggcatcc tggtgctgga tctcaccgta
     5101 cagggcgtgc atatcactaa ccagacggta atttatcgaa tacaccctga tgcgcgtaat
     5161 cgcctgaccg caggttacat gaccagctac tttattggcg gtgccgccgg ttcgctaatt
     5221 tcagcctcag cctggcaaca tggcggttgg gctggcgttt gtctggctgg cgcgacgatt
     5281 gccctggtta acttactggt ctggtggcga ggttttcatc gtcaggaagc cgcaaattaa
     5341 gcaatcagca accgttttcg tggctttaca cttataaggg tgttaagaag cccatcagtc
     5401 tgataaggtt aagatattca ttcagtctat ttataatatt aacaatcgtt aagcgtacac
     5461 tctatggaaa gccctactcc acagcctgct cttggttcgg cgaccttcat ggaaggatgc
     5521 aaagacagtt taccgattgt tattagttat attccggtgg cctttgcgtt cggtctgaat
     5581 gcgacccgtc tgggattctc tcctctcgaa agcgtttttt tctcctgcat catttatgca
     5641 ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg ggagtagttt gtgggttgct
     5701 gcactgaccg tcatggcaat ggatgttcgc catgtgttgt atggcccgtc actgcgtagc
     5761 cgtattattc agcgtctgca aaaatcgaaa accgccctgt gggcatttgg cctgacggat
     5821 gaggtttttg ccgccgcaac cgcaaaactg gtacgcaata atcgccgctg gagcgagaac
     5881 tggatgatcg gcattgcctt cagttcatgg tcatcgtggg tatttggtac ggtaataggg
     5941 gcattctccg gcagcggctt gctgcaaggt tatcccgccg ttgaagctgc attaggtttt
     6001 atgcttccgg cactctttat gagtttcctg ctcgcctctt tccagcgcaa acaatctctt
     6061 tgcgttaccg cagcgttagt tggtgccctt gcaggcgtaa cgctattttc tattcccgtc
     6121 gccattctgg caggcattgt ctgtggctgc ctcactgcgt taatccaggc attctggcaa
     6181 ggagcgcccg atgagctatg aggttctgct gcttgggtta ctggttggcg tggcgaatta
     6241 ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat gcccgcccaa ccaaacgtgg
     6301 cgcggtaggt attttgctcg acaccattgg catcgcctcg atatgcgctc tgctggttgt
     6361 ctctaccgca ccagaagtga tgcacgatac acgccgtttc gtgcccacgc tggtcggctt
     6421 cgcggtactg ggtgccagtt tctataaaac acgcagcatt atcatcccaa cactgcttag
     6481 tgcgctggcc tatgggctcg cctggaaagt gatggcgatt atataaccca caagaatcat
     6541 ttttctaaaa caatacattt actttatttg ttactgtcgt tactatatcg gctgaaatta
     6601 atgaggtcat acccaaatgg atagttcgtt tacgcccatt gaacaaatgc taaaatttcg
     6661 cgccagccgc cacgaagatt ttccttatca ggagatcctt ctgactcgtc tttgcatgca
     6721 catgcaaagc aagctgctgg agaaccgcaa taaaatgctg aaggctcagg ggattaacga
     6781 gacgttgttt atggcgttga ttacgctgga gtctcaggaa aaccacagta ttcagccttc
     6841 tgaattaagt tgtgctcttg gatcatcccg taccaacgcg acgcgtattg ccgatgaact
     6901 ggaaaaacgc ggttggatcg aacgtcgtga aagcgataac gatcgccgct gcctgcatct
     6961 gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt ttaccaccgc agcataactg
     7021 cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa gatcagctcg agcaaatcac
     7081 ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac ggtgtggttc tcgaagcgat
     7141 gagctaacgc gtcatctcgc tcaaaaatcc agatttataa aagaaaaaat gaccggccag
     7201 catcgcaaca tgctggcctt tttggcaagc aggtcggctc agccgatgag ttaagaagat
     7261 cgtggagaac aatatgagcg caaatgcgga gactcaaacc ccgcagcaac cggtaaagaa
     7321 gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg ctctttataa ttattgccgt
     7381 agcgataggg atttattggt ttttggtact gcgtcatttc gaagaaaccg atgacgcata
     7441 cgtggcaggg aatcagattc aaattatgtc tcaggtgtct ggcagcgtga cgaaagtctg
     7501 ggccgataac accgattttg taaaagaagg cgatgttctg gtcactctcg acccgacaga
     7561 tgctcgccag gcgtttgaaa aagccaaaac tgcactggct tccagcgttc gccaaaccca
     7621 ccagctgatg attaacagca aacagttaca ggcgaatatt gaggtgcaaa aaatcgccct
     7681 cgcgaaagca caaagcgact acaaccgccg tgtgccgctg ggcaatgcca acctgattgg
     7741 tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt gcccaggcgc aactggacgt
     7801 cgctattcaa caatacaatg ccaatcaggc gatgattctg gggactaaac tggaagatca
     7861 gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc tggctggcgc tggagcgtac
     7921 ccgtattgtc agtccgatga ccggttatgt ctcccgccgc gcggtacagc ctggggcgca
     7981 aattagccca acgacgccgc tgatggcggt cgttccagcc accaatatgt gggtggatgc
     8041 caactttaaa gagacgcaga ttgccaatat gcgtatcggt cagccggtca ctatcaccac
     8101 ggatatttac ggcgatgatg tgaaatacac cggtaaagtg gttggtctgg atatgggcac
     8161 aggtagcgca ttctcactgc ttccggcgca aaatgcgacc ggtaactgga tcaaagtcgt
     8221 tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag ctggagcaat acccgctgcg
     8281 cattggtttg tccacgctgg tgagcgtcaa taccactaac cgtgacggtc aggtactggc
     8341 aaataaagta cgttcaacac cggtcgcggt aagtaccgtg cgtgaaatca gcctggcacc
     8401 tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct ggctaatcca gaggtgcgtg
     8461 tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt cattatgacg attgcgctgt
     8521 cactggcgac attcatgcag gtgctggact ccaccattgc taacgtggcg atccccacta
     8581 tcgccgggaa tctgggctca tcgctcagcc agggaacgtg ggtaatcact tctttcgggg
     8641 tggcgaatgc catctcgatc ccgcttaccg gctggctggc aaagcgcgtc ggggaagtga
     8701 aactgttcct ttggtccacc atcgcctttg ctattgcgtc gtgggagtgt ggtgtctcca
     8761 gcagcctgaa tatgctgatc ttcttccgtg tgattcaggg gattgtcgct gggccgttga
     8821 tcccactttc gcaaagtctt ttgctgaata attacccgcc agccaaacgc tcaatcgcgc
     8881 tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg cggcccaatc ctcggcggtt
     8941 atatcagcga taattaccac tggggctgga tattcttcat caacgtgcca attggcgtgg
     9001 cggtggtgtt gatgacactg caaaccctgc gcggacgcga aacccgcacc gaacggcggc
     9061 ggattgatgc cgtggggctg gcactgctgg ttattggtat cggtagcctg cagattatgc
     9121 tcgaccgcgg taaagagctg gactggtttt catcacagga aattatcatt cttaccgtgg
     9181 tggcggtggt ggctatctgc ttcctgattg tctgggagct gaccgacgat aacccgatag
     9241 tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg ctgcttgtgt atcagcctcg
     9301 cgtatatgct ctacttcggc gcgattgttc tgctgccgca gttgttgcag gaggtctacg
     9361 gttacacggc gacctgggca ggtttggcct ctgcgccggt agggattatt ccggtgatcc
     9421 tgtcgccgat tatcggccgc ttcgcgcata aactggatat gcggcggctg gtaaccttca
     9481 gctttattat gtatgccgtc tgcttctact ggcgtgccta tacctttgaa ccaggtatgg
     9541 attttggcgc gtcggcctgg ccgcagttta tccaggggtt tgcggtggcc tgcttcttta
     9601 tgccgctgac caccattacg ctgtctggtt tgccaccgga acgactggcg gcggcatcga
     9661 gcctctctaa ctttacgcga acgctggcgg ggtctatcgg cacgtcgata accacgacca
     9721 tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac tgagtcggta aacccgttca
     9781 acccgaatgc ccaggcgatg tacagtcaac tggaagggct tgggatgacg caacagcagg
     9841 catcaggctg gattgcccag cagatcacta atcaggggct gattatttcc gccaatgaga
     9901 tcttctggat gtcagccggg atattcctcg tcctgctggg gctggtgtgg tttgctaaac
     9961 cgccctttgg cgcaggtggc ggcggaggtg gtgcgcacta agtacaacta agccagttca
    10021 tttgaactgg cttttttcaa ttaattgtga agatagttta ctgactaaat gtgcagttcc
    10081 tgcaacttct ctttcggcag tgccagttct tcgttgctgt tgatgcgtac gtcacgttcc
    10141 agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt gcatctggta agtgccacac
    10201 tggtagacgt tcagctccgg gatctgattc tgatcctgca ctttcagcac gtcttccatt
    10261 gccgctttcc aggcatcagc aacacgctgc tcatctggcg taccaatcag actcatataa
    10321 aaaccggtgc ggcagcccat tggcgagata tcgataatct ctacaccatt accgttaaga
    10381 tggttacgca taaaaccagc aaacaggtgc tccagagtat ggctccctct ttctggcatc
    10441 acttctttgt tcggcacgca gaagcgcaga tcgaacacgg tgattgcgtc gccatgcggg
    10501 gtgttcattg ttttcgccac ccgaactgca ggcgcttcca tccgggtatg atcgactgtg
    10561 aagctatcta acaacggcat ttagccacct ccggtaattt ttttaaaaat tttctgaact
    10621 ctttcttccc aggcgagtct gagtatatga aagacgcgca tttgttatca tcatccctga
    10681 attcagagat gaaattttgg ccactcacga gtggcctttt tcttttctgt caggcgtgtt
    10741 tttccagcca caccgcaaac ggttcggtat cagcggtttc catttcctgc tgacggcgtt
    10801 cagatgcctc gcgctcggct acaaaatcct cttcgcgcag aatttccaac ggctcttcac
    10861 gcagcagatt acggtaggct tctgcaaacg ctttgcctgt tccgccaata ccagtatcaa
    10921 tcatagacct taagatacgg gcagagaaag tcagatcggg attatcgaag caggcgacca
    10981 gttcatcaca cactttctga tacgctttgc cgccgttaat gctatccaac gtttgcgcga
    11041 cgcgtttcag atcgcggaac agatctttac ccacctgcgg taacgggaac tgcgcggttt
    11101 cgcagccgat acccagcgtc aggcccggtt tgcgaccttc gaggatcacc cggttccagt
    11161 taacgcgtgt acaggcaagt tcgctactgc tcatttccgg tgcatcagcc agcgcacacc
    11221 agaccataaa caggtcgagg aatcgcacct gctgttcatc tacaccaatc ggcgagaacg
    11281 ggttgatgtc cagcgaacgc acttcaatat attcaatgcc gccacgtaac agcgcatcag
    11341 aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat cggcgcatac agttcgtttt
    11401 caatctgcag cacgttgcta ttgatttgca gcctcttacc gtctttctca ataccaatct
    11461 tcgcgtactc ttccgatggc gttttgattg cctgtttaag gcccgctacg tactcgtaaa
    11521 gatcgttgaa ggtaatacca agattgcttt gcgatttatt ggtatagccg agatcgctca
    11581 aacgaagaga ggtcgcatac ggcaggtaat acataccgca ctcggttttc tcaaacggca
    11641 gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc cggagatgca ccaaacagat
    11701 aaggaatgac ccaaccgaaa cgatagtaat tgcggataac gcggaaatag cccgcagaaa
    11761 ttttctcttt ggcatcagtg cccgagatat caccgcactt cgcttgccag aatgccattg
    11821 gcaaagagaa attgtagtgc acgccggaaa tggtttgcat cagcgcgccg tagcgatttt
    11881 tcagcccttc acgatacagc gttttaaagc gtccggtgtt agaagtgccg tactgtgcca
    11941 gttcgatgtc ctgaccttct gcgatgtagc atggcatact taacggccac atccgctcat
    12001 cgcccatatt gcgcgccgta taacgatgca gatcgcgcat aaaggtcaac atatgttcaa
    12061 tatcaccatc cactggtgta atgaattcca gcaatgcttc cgcaaaatcg gtagtaatcc
    12121 atttgtgcgt cagtgcggaa cctaatgctt caggatgacc tgttgttgcc tgtgtgccat
    12181 cagcattaac acgcaaagtt tcgcgctcca gcccacgctg tatccccttt aacgcctgag
    12241 gatgtttttc cagccaggcc agcgcctgtg atacgtccgg gatcaaattg acctcccgcc
    12301 tgtcaaaatc gttttaatta gcataactgt aatggtgacc atatgtgcag gcctacaatt
    12361 agtgccacca catcatgccc tgaacggtag ctgctgcaac tgcaacatag cgtaaagctt
    12421 taccaaggca caaaaaaaag attaccggtc cccacgagat gcgcatccat cccgctaaca
    12481 gacacagtaa atcgccaacc acgggcatcc agcttaataa tagcgtgact gcaccatagc
    12541 gtttcagcca gccagtagct ttctcttgcc agcgcgatgt tttacgcaat ggaaagaaac
    12601 gcccaaggat aacgttagtt aaccctccaa ggctattacc cattgttgct gttaaaacta
    12661 aaacccaggg ataactgatc ccggaaagca acattgccac cagcacgact tcggagttgc
    12721 cgggtaatag tgtagcgctg agaaaactac tggcaaacaa cgagaaaagc gataacgctt
    12781 cactcacagc aagcgaacat ccacggcgtc catgcctgct gcacgggccg cctgaatacc
    12841 gaaatcggca tcttcaaaga ccacacactg cgtcggttgc acgcccatac gctgcgcgca
    12901 caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg tgatcagcag cgacgacggc
    12961 gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc tcagcgatgg cgctttcact
    13021 ccccgttcct acagccattg ggcgacgacc atgccaactt ttcaccactt caacaagagg
    13081 aagcggctcg acactatcca gcagcatact tcttactgct tctgtttttt cacgcgctaa
    13141 cgcatgcggg gcgagatcgg cctgattcag ctcaataatt gcctgagcaa tacgccaggt
    13201 gggcgatcca ttaagcgcaa tcattgcctg aacatcgtac tgaagaccgt agtgccctaa
    13261 tacttcgcgc cacgctttac ggtgcgtagg ctccgtatcc aggattgtgc catccatatc
    13321 aaaaattaaa cctgcataac gctcgtacat ggtcttctcg caagtcgaaa aatatgacgc
    13381 tactttagcg taattgcttg attttgtcgc tgatggcgaa ggagaagaaa attcaggaaa
    13441 tggataaagt agttatttag aaatgagata tttttgaagg aaatttttgg gagaagatgg
    13501 tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc
    13561 tgagctacgg atgcatcggg aaaatttatt ttactgctga tattgatatc gctactaaag
    13621 ccatatcaag taagagatgg tgcatccggg aggattactc gccttgcggc tcgcccttcg
    13681 ggccgttgct aaagcaacgt tatcatccct agtgcctgcg gttaattccg ctacctcttc
    13741 gataccttca ttgctgaaga tttcggagaa tatggtgcat ccgggaggat tcgaacctcc
    13801 gaccgctcgg ttcgtagccg agtactctat ccagctgagc tacggatgca tcgggaaact
    13861 tactttactg cagatttttt gataccgcta ctaaagccgt atcaagtaag agatggtgca
    13921 ttcgggagga ttactcgcct tgcggctcgc ccttctggcc gttgctaaag caacgttatc
    13981 ctccctggtg cctgcggtta taccgccacc tcttccatac cttcatcgct gaaagtacgt
    14041 agaatatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta gccgagtact
    14101 ctatccagct gagctacgga tgcaaatggc ggtgaggcgg ggattcgaac cccggatgca
    14161 gcttttgacc gcatactccc ttagcagggg agcgccttca gcctctcggc cacctcacca
    14221 cacgcctctt acgagtgctt cgaagaactt gtttatcgct catcgtcgct gcgtggcgca
    14281 catattactt tctgagactt ataagtcaaa caatttttcc cacactttta tcgtttgcac
    14341 acttcacgct caattagtct gtaaaaacgt caaaaagagt gttttatcta cagaaaaacg
    14401 gaggtctggc agatagcagt aatgcaaaaa aatggagact taagttgaat gaacgggagt
    14461 aaagcgaaaa gactatagag tgaaggagaa attttgaggg tgcgtctcac cgataaagat
    14521 gagacgcgga aagattagta actggactgc tgggattttt cagcctggat acgctggtag
    14581 atctcttcac ggtgaacaga aacttccttc ggggcattta cgccaatacg tacctggttg
    14641 cccttcaccc ctaaaactgt cacggtgacc tcatccccaa tcatgagggt ctcaccaact
    14701 cgacgagtca gaatcagcat tctttgctcc ttgaaagatt aaaagagtcg ggtctctctg
    14761 tatcccggca ttatccatca tataacgcca aaaagtaagc aatgacaaac acattacatg
    14821 taagcagtca tggcattaca ttctgttaaa cctaagttta gccgatatac acaacttcaa
    14881 cctgacttta tcgttgtcga tagcgttaat gcgaatgccg tgaagcgagt ccacggcatt
    14941 gcctgacgct tatattattg caatttcgcg ctgacccagc ctttcacact ggctaacgct
    15001 gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca tgtcaggacg tccaccaccc
    15061 ttgccgccca cctgctgagc gaccatacca atcagttccc ctgctttcac acgatctgtg
    15121 acgtccttag atacgcctgc aatcagagaa accttacctt cggctaccgt tgccagcacg
    15181 ataattgtcg accccagctg attttttaaa tcgtcaacca tggtacgcaa cattttcggc
    15241 tcaacaccgc taagctcgct aaccaacagc ttaacaccat taacatcaat tgccttactg
    15301 gaaagatttg cgctctcctg tgcggcagct tgttctttaa gctgttgtag ttctttttct
    15361 aactgacgcg tacgttccag tactgagcgc actttatccg ccagattatt gctatcgcct
    15421 ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg catgaacggt ggtgatagcg
    15481 ccttctccgg ttaccgcttc gatacgacga acgcctgcag cagtacccga ttcagagatg
    15541 atgcggaaca gaccaatatc accagtgcgg ctggcgtgag taccgccaca cagctcggtg
    15601 gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat acttctcgcc gaacagcgcc
    15661 atcgcacctt tcgctttcgc cacttcgaga tccatgatgt tggtttcgat cggcaaattg
    15721 cgacgaatct gtgcgttcac caggtcttcg accgcacgaa tctcttccgg tttcatcgct
    15781 tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa ccagtgaacc tttctgcgat
    15841 acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca ggtgcgttgc ggagtgattc
    15901 agacgaatac gggcgcgacg agcctcatca acatcagcct gcacagcgtc gcccactttc
    15961 agagaacccg cagcaagttt accgatgtga ccaatcgcct ggccgtattt ctgcgtatct
    16021 tccaccgcga aggagaagtt agcgtctttc agttcgcctt tatcaccaac ctgaccgccg
    16081 gattccgcat agaatggtgt ttgatctagc acgaccacag cttcctggcc tgcattgatg
    16141 gcatcaaccg ctttaccatc aacaaacagc gcggtcactt tgccgttcag ttccagatgg
    16201 tcatagcctt taaattcaga tgcactatca acacggatca ttgcgttgta atcggcacca
    16261 aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca ttgctgcttc aaaaccagct
    16321 tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg tcaggtcaac cgggaagcca
    16381 taggtgtcgt acagacggaa agccgtttca ccatccagcg tatcaccaga aagttttgcc
    16441 agctcttcat ccagcaacgc cagaccgcgc tccagagtac gggcaaactg ctcttcttca
    16501 gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca ggtcttcacc cgcagagccc
    16561 ataacgtcga tcagcggacc aaccagtttg tagaagaagg tttctttcgc gccgagcata
    16621 ttgccgtgac gcactgcgcg acgaatgata cgacgcagta cataaccacg gttttcattg
    16681 gacggcatta cgccatccgc gatcaggaac gcacaagaac gaatgtggtc agcgattacg
    16741 cgcagcgatt tattgctcag atcggtcgcg ccagtgactt tcgctaccgc ctggatcaac
    16801 gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt gcagcaccgc agcaatacgc
    16861 tccagaccca taccggtatc tacagacggc ttcggcagcg gttccatcgt gccatctgcc
    16921 tggcggttga actgcatgaa gacgatgttc cagatctcaa tgtagcggtc gccgtcttct
    16981 tccggacttc ccggagggcc accccaaatg tggtcgccgt gatcgtagaa gatttcggtg
    17041 cacgggccgc acggaccagt gtcacccatc tgccagaagt tgtcagatgc gtatggcgca
    17101 cctttgttat cgccgatgcg aataatacgt tcgcgcggga tcccgacttc tttttcccag
    17161 atttcgtagg cttcgtcgtc gctttcatag acggtaaccc acagacgctc tttcggcagg
    17221 gcaaaccatt tttcgctggt cagcagttcc catgcaaact gaatggcatc gtgtttgaaa
    17281 tagtcgccga agctgaagtt gcccagcatt tcgaagaagg tatggtgacg cgcggtgtaa
    17341 ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca cgcagcgttg ggaagtggta
    17401 gcgcgggaat aattacgctt gtcgagccca aggaacacat ccttgaactg attcatcccg
    17461 gcgttggtaa acaacaaagt tgggtcgtta tggggtacca gggagctgct ggcaactacc
    17521 tgatgtccct tactatggaa aaagtcgaga aacgcctgac ggatctcagc ggtgctcttg
    17581 ctcataatta tcctgaaatc aagctaacga aatatcgcca ccagctccag cgtgtcttaa
    17641 ccgccgggct ggtaactgaa aagtgggaat aagataagtt ttcttgactg ggaagtaaaa
    17701 taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc tggatatctt ccatcagata
    17761 gccacgatag agcagaaaac gctggatctt aactttttct gaaaagacag ttggcaaagg
    17821 ttcatcatat tttcgcgtcg cctgatcgcg cgccagtgcg caccagtcga tgtcacattc
    17881 acgcatcgct ttttctgtcg cttcgcggga aatacctttc tgattcagtt cctgacgaat
    17941 acgtgcaggt ccataacctt tgcggctacg gctggcgata aagcgcgcaa caaatcggct
    18001 gtcatcgaga tagccatgtt catggcacca tgcaataacg cgctcgtaat cttctgccgt
    18061 agcatcaatc tcttctgggc catttttgcc cataatcggt gccgcgagtt tacgtcgcag
    18121 ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc gcacgatcca acaggcgagc
    18181 atatgccggg cgacgggacg ttgattctgt catggcatat ccttacaact taaaaaagca
    18241 aaagggccgc agatgcgacc caggttttgc tgataaacag atttaatagt caatgagtgt
    18301 gcagaatgtt cccgcaggcc caattcgtta gaggatcgag gtgagagtaa tttttcacgc
    18361 cagtgctggc ctgttcccct caccctaacc ctctccccaa aggggcgagg ggaccgtccg
    18421 tgcacacatt atctattatc atcaataacg aacgattaaa aatcttcgtt agtttctgct
    18481 acgccttcgc tgtcatctac agagaaatcc ggcgttgagt tcgggttgct cagcagcaac
    18541 tcacgtactt tcttctcgat ctctttcgcg gtttccgggt tatctttcag ccaggcagtt
    18601 gcattcgctt taccctgacc gatcttctca cctttgtagc tgtaccacgc gcctgctttc
    18661 tcgatcagct tctcttttac gcccaggtca accagttcgc cgtagaagtt gataccttcg
    18721 ccgtagagga tctgaaattc agcctgttta aacggtgcag cgattttgtt cttcaccact
    18781 ttcacgcggg tttcgctacc caccacgttt tcgccctctt tcaccgcgcc gatacgacgg
    18841 atgtcgagac gaacagaggc gtagaatttc agcgcgttac caccggtagt ggtttccggg
    18901 ttaccgaaca tcacaccaat tttcatacgg atctggttga tgaagatcag cagcgtgttg
    18961 gactgcttca ggttacccgc cagcttacgc atcgcctggc tcatcatacg tgccgcaagg
    19021 cccatgtgag agtcgccgat ttcgccttcg atttccgctt tcggcgtcag tgccgccacg
    19081 gagtcaacga cgataacgtc tactgcacca gaacgcgcca gggcgtcaca gatttccagt
    19141 gcctgctcgc cggtgtccgg ctgggagcac agcaggttgt cgatatcgac gcccagttta
    19201 cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa acgcacaggt tttaccttca
    19261 cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt taccggaaga ttccggtccg
    19321 tagatttcga cgatacggcc catcggcaga ccacctgccc caagcgcgat atccagtgaa
    19381 agcgaaccgg tagagatggt ttccacatcc atggaacggt cttcacccag gcgcatgatg
    19441 gagcctttac caaattgttt ctcaatctgg cccagtgctg ccgccaacgc tttctgtttg
    19501 ttttcgtcga tagccatttt tactcctgtc atgccgggta ataccggata gtcaatatgt
    19561 tctgttgaag caattatact gtatgctcat acagtatcaa gtgttttgta gaaattgttg
    19621 ccacaaggtc tgcaatgcat acgcagtagc ctgacgacgc accgcatcac ggtcgccgct
    19681 gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg gcaaaagcaa accagacggt
    19741 gccgacaggc ttctcttcac tgccgccatc cggcccggcg ataccactaa tagacacggc
    19801 ataatcagca cgagccgctt tcagtgcgcc tatcaccatt tccaccacga cgggttcact
    19861 caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg atcatctgcg ctttggcttc
    19921 gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag ctaccggcaa tatcggtaat
    19981 cactttcgct acccaaccac cggtacaaga ctcggcagtt gttacggttg cgccacgggc
    20041 tttcagcgcc tgcccaacct gttcacttaa ctgcatcagt tcactgtcag tcatcacaac
    20101 ctctgtcagt caaaaattta tgccggacaa gatagcactt tcgtcaggaa gatggtgttg
    20161 gcgttgcaat tttacgagga ggattcagaa aaaagctgat tagccagagg gaagctcacg
    20221 cccccctctt gcaaatagtt actgcacgcg cgccagcgcc acggcttgtc ctaactgcca
    20281 gaccgccatt gcgtaatggg tgctgtggtt ataacgggta atggtgtaga agttcggcag
    20341 accgtaccag tactggtaac cggtaccgac atccagacgc agcaggctgg cttgttgatg
    20401 gttgcccagc ggctgctgtg gcgttaaacc tgccgtggca agctgcgaaa tgctgtacct
    20461 ggttttgaag ccatttggca agcctggagc ctgaccgttt gccattaccg cgacctgatc
    20521 gcctttcacc cagccgtgcg ctttgaaata gttcgccacg ctaccgatcg catcaaccgg
    20581 atcccacagg ttgatatgcc cgtcgccgct gaaatctacc gcatattgtt tgtaagacga
    20641 cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa cctttcagat tgagcggatc
    20701 gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc tcgccagaga aatactccgc
    20761 gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg aggatgcgag ttttccccat
    20821 cacgcgcccc cagcgggttt caacgccgat aatcccgacg ataatttccg gcggtacgcc
    20881 atacacctgc cacgcgcgat tcaacgcatc ttcatactga ttccagaaaa ccacaccgtt
    20941 ctgcacgttg tccggcgtaa taaatttttt gcgataacgc agccatgcgc cgttcggacc
    21001 tgatggcggt ttcaccgatg tggttggtgc ctggttatcc atcagccgca gtaccgaatc
    21061 cagacgcttc gcctgggaga gaatttcctg caactgctga cgatcgaaac cgtgtttgtt
    21121 caccattttg tcgatgaact gctgggcatt cgggttatta gcgaaatcgc cgcccatctg
    21181 catcacattg tgctgcggct caagcaggaa gccgccagac ggcgttccgg tggtcgtttc
    21241 agtctcagta ggttttggct tgctgctaca ggcggcaagc aacacaaaaa ggggaagcaa
    21301 tgttacataa cgacgcttga acatgagggg tccatttaac agattcaacc aggggcaagt
    21361 atggtaaagc atcacgcccc gcacaaggaa gcggtagtca ctgcccgata cggactttac
    21421 ataactcaac tcattcccct cgctatcctt ttactcaaac ttccaaatta aaatatttat
    21481 ctttcatttt tagattaaaa taacactttt aaatctttca atctgattag attaggttgc
    21541 cgtttggtaa taaaacaata aatcctgaag gagagaacaa tgatagaaac cattactcat
    21601 ggtgcagagt ggtttatcgg gctgttccaa aagggcggag aggtgtttac cgggatggtg
    21661 accggcattc ttccgctgtt gattagcctg ctggttatca tgaacgcact gattaatttt
    21721 atcggtcagc atcgtattga acgttttgct caacgttgcg ccggtaaccc tgtttcccgt
    21781 tacctgctgc taccgtgcat tggcacgttt gtcttttgca atccgatgac cttaagccag
    21841 ggtcgcttta tgccggaaaa gtacaaaccc agctactacg cggcggcctc ttatagctgc
    21901 cactcaatga atggcctctt tccccatatc aaccctggcg aactgtttgt ttatcttggc
    21961 attgccagcg gtctgacaac gctgaacctg ccacttggcc cactggcgtt gagctatctg
    22021 ctggttggtc tggtcaccaa cttctttcgc ggctgggtga ccgatctgac caccgccatt
    22081 tttgagaaaa agatgggcat tcaacttgaa caaaaagttc acctggcagg agcaacatca
    22141 tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg cggcccgctt gagctggaag
    22201 ccacgccggg caaaaaaatc gtctatatca ccgccggtac ccggcctgcg attgttgaca
    22261 aactggcaca acttactggc tggcaggcta ttgacggatt taaagaaggt gaacccgcgg
    22321 aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac attacgctgc ggcatctatc
    22381 cgaaacgacg tattcccacc attaatatcc actcgacggg caagtccggt ccgctggcgc
    22441 agtacattgt ggaagatatt tatgtctctg gcgtaaaaga agaaaacatc actgtagtgg
    22501 gtgatgcgac accacaaccc tcttccgtgg gccgtgacta tgacaccagt aagaaaatca
    22561 ccgaacaaag cgatggttta ctggcgaagg tgggaatggg catggggtcc gccgttgcgg
    22621 tgctgtttca atctggtcgt gacaccatcg acactgtatt aaaaaccatt ctgccgttta
    22681 tggcattcgt ctcggcgctc attggcatca ttatggcttc tggccttggt gactggattg
    22741 cccacggtct tgctccgctg gcgagccatc cactgggtct ggtcatgctg gcgctcatct
    22801 gctccttccc actgctttca cctttcctcg gcccaggcgc agttatcgca caggttatcg
    22861 gcgtattgat tggcgtgcag attggtctcg gcaatattcc gccgcatctg gctttaccgg
    22921 cactgtttgc catcaacgcg caggcggcct gcgacttcat cccggtcggt ttgtcgctgg
    22981 cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc tgtactggtg agccgctttt
    23041 taaccggcgc accgactgta ctgatcgcct ggtttgtctc cggttttatc tatcaataga
    23101 ggctgaaaca tgaccgttat ttataagacc accatcaccc gtatcggcgc gagtgccact
    23161 gacgccctca gcgaccagat gctcatcacc tttcgtgaag gcgcgccagc ggacctcgaa
    23221 gagtattgct tcattcattg ccacggcgag ttgaaaggtg cactccatcc cggtttgcaa
    23281 tttacgctcg ggcagcatcg ctatccggtg accgctgttg gcagcgtggc ggaagacaac
    23341 cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt taaacgaagc ggaatttccg
    23401 ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg cgccgggatc ggttttgaag
    23461 tttgaatctg ttaaggagta aaaaatgaat caggttgccg ttgtcatcgg tggtgggcaa
    23521 accttaggcg cgttcctgtg ccacggtctg gttgccgagg ggtatcgcgt cgcggttgtc
    23581 gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa ttaacgccga atatggtgaa
    23641 agtatggcgt acggttttgg tgctgacgcc actagcgagc aaagcgttct ggcgctctct
    23701 cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg tctatagcgc cggaatagca
    23761 aaagcagcct ttatcagcga cttccagctc ggcgattttg accgttcgct acaagtgaat
    23821 ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt tgatgatccg cgacgggatt
    23881 cagggtcgca ttattcagat caactcgaaa tccggcaaag tgggcagcaa acacaactct
    23941 ggctacagcg cagcgaaatt tggtggcgtc gggctgactc aatcactggc gctggatctg
    24001 gcggagtacg gcattacggt gcattcactg atgctcggta acctgctgaa atcgccgatg
    24061 ttccagtcac tgttgccaca atacgcgacc aagctgggta tcaaaccgga tcaagtcgag
    24121 cagtattaca ttgacaaagt accgctcaaa cgcggctgcg attatcaaga tgtgctgaat
    24181 atgctgctgt tctacgccag tcctaaggcg tcgtactgca ccggacagtc gatcaatgtc
    24241 accggcggtc aggtgatgtt ctgatcaaca gcggagatcc attaaggatc tccgtgagac
    24301 tatagaatgc ctgatgcgct acgctcatca ggcatacagg acttccgcca ctacattaag
    24361 gaaaagttat ggtatccgca ctcatcaccg tcgccgttat cgcctggtgt gcgcaactgg
    24421 ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt cgacacacta tgccagcaag
    24481 ggcgggttgg cgtgggtcgt tccagcgggc gctttaaacc gcgggtcgtg gtcgccatcg
    24541 gctggacgat cagcagcgca tcgtcgacac cttgtttatg aaaggactga ccgtcttcgc
    24601 ccgaccgcaa aaaattcccg caattaccgg tatgcatgcg ggtgatttac agcccgatgt
    24661 gatctttccc catgatccac tatcacagaa tgctctatca ttggcgctta aactgaaacg
    24721 tggataattt cgttgtgaat gttacttgct tgcgaagtta tcattttgaa acctaaatca
    24781 ggtaatcacg cccatgaaac ctcgtcagcg tcaggccgcc attctggagt atctgcaaaa
    24841 gcagggtaaa tgctcggttg aagaattggc gcaatacttt gacaccacag gcacaaccat
    24901 tcgcaaagat ctggtcattc tggaacatgc cggaaccgtc attcgtactt atggcggagt
    24961 ggtgttgaat aaagaggaat ccgatccgcc tatcgatcat aaaacactca tcaacaccca
    25021 caagaaagag ctgattgcag aagctgccgt tagttttatc catgatggcg attcgatcat
    25081 tcttgatgct ggcagtaccg ttttgcagat ggttcccctg ctctcgcgct ttaataacat
    25141 cacggtgatg accaacagcc tgcatatcgt caatgcgcta tccgaactgg ataacgaaca
    25201 aactatcctg atgccaggcg gaacgtttcg taaaaaatcg gcctcatttc acggacagct
    25261 ggcagagaat gccttcgaac atttcagttt cgataaattg tttatgggca ccgacggcat
    25321 cgatctcaat gcgggcgtaa ccaccttcaa cgaggtttat accgtcagta aggcaatgtg
    25381 caatgccgcg cgcgaagtga ttttgatggc ggactcatca aagtttggcc gtaaaagccc
    25441 caacgtagtt tgcagtcttg aaagcgtcga taagctgatt accgacgcag gtatcgatcc
    25501 ggcgtttcgt caggcgctgg aagagaaagg gatcgatgtg atcataaccg gagagagcaa
    25561 tgagtgaagc actactgaac gcgggacgtc agacgttaat gctggagttg caggaagcaa
    25621 gccgtttacc ggaacgtctg ggcgatgatt ttgttcgcgc cgccaatatc atcctgcact
    25681 gcgaaggcaa agtggtggtt tcgggaattg gcaaatcggg ccacattggt aagaaaatcg
    25741 ccgcaacact tgccagtacc ggcacaccgg ctttttttgt ccatccggca gaagcactgc
    25801 acggcgatct ggggatgatt gaaagccgcg atgtgatgct gtttatctct tactccggtg
    25861 gcgcgaagga actggatctg attattccgc gtctggaaga taaatctatc gcgctgctgg
    25921 cgatgaccgg caaaccgacg tcaccgctgg gcctggcggc aaaagcggtg ctggatatct
    25981 ccgtagaacg cgaagcctgc ccgatgcacc ttgcgccgac ctccagcacc gtcaataccc
    26041 tgatgatggg tgacgcgctg gcgatggcgg tcatgcaggc gcgcggattt aatgaagaag
    26101 attttgcccg ctcccaccca gccggggcac tgggcgctcg cttgctgaat aaagtgcatc
    26161 atctgatgcg ccgtgacgat gccatcccac aggtggcgtt aaccgccagc gtgatggatg
    26221 cgatgctgga actcagccgc accggtctgg ggctggtggc ggtatgtgac gctcaacaac
    26281 aggtacaagg cgtctttacc gacggcgatt tacgtcgctg gctggtaggc ggcggcgcac
    26341 tcaccacgcc agttaatgaa gcgatgacga caggcggcac cacgttacag gcgcaaagtc
    26401 gcgccatcga cgccaaagag atcctgatga agcgcaaaat cactgccgca ccggtggtgg
    26461 atgaaaacgg caaactcacc ggcgcaatta acctgcagga tttctatcag gccgggatta
    26521 tttaatcctt cattcccaga cgtttcgcca gccgatgcag gttggcgacg tcggtttcca
    26581 gcatccgcgc gcaggcagcc cagttatgat gattttgtgc cagtgcctgg cgaatagttt
    26641 cacgctggaa cgcttctgtc gcttcacgca ggttttgctt aacaacgggc accgccgccg
    26701 cttctggcgg cggcaacgtc acctcaggaa aagcaaaatg ttgcgcctca agaatcactt
    26761 catcgccgtt gcgggtggct ctcgccagca ctaccgcccg atgaatagca tgttccagtt
    26821 cgcgcacgtt ccccggaaaa cgatagtgtt gcagtaaatt tcgcgctccg gcacttaata
    26881 ccacgcggga gagccccagc cgcaaacgac actgttcgca gaaatacccc gccagcagaa
    26941 tgacatcatc gccccgctca cgcagcggcg gcaccgaaag tggaaacacg ctcaggcgat
    27001 gaaacaagtc agcgcgaaat cgccctgcca gcacctcttc gcgtaagtcg cggttagtcg
    27061 ccgccagcac gcgcacatcg acccgcaaac tacggtcatc gccaacgcgc tgaatatcgc
    27121 catactgcaa caccctcagc agcttggcct gcaatgccaa cgacaactcg ccgatctcat
    27181 ccagaaacag agtgccgtta tccgccattt caaacttccc gctgcggtta ctgatagcgc
    27241 cagtaaacgc tcctttcaca tgcccgaaca actcactttc cgccacactt tccggcagtg
    27301 cagcacagtt gagatagacc agcggattca ccgcccgtgg cgaggcttca tgaatcgctt
    27361 tcgccaccag ctccttaccg gttcccgtct caccgctgat caggacgttg agatcggacg
    27421 ccgccacaat ctcaatctct tttttcagtt gcgtcatgcc aggggacaag ccaatcatct
    27481 gcgtctgttt caccgcttca aacggcgtgg catcgcctgg catcatattc tggctttcca
    27541 gttgttcaat cagcaacgca ttgcttaacg ctcccgccgc cagcgcagca atcagccgta
    27601 gctcttcgtc gctgaaaaca tcgaactgat cgggctgcat cccgtcgagc gtcagtgcgc
    27661 cgatcaggtt ttgcccggca aacaatggca gaccaacgca ggcgtgaacc ttcagactct
    27721 cctgcccagg aatcaaaccg tcatagggat cgggcaattc gctgtctgcg ggaaagcgca
    27781 ccacatcccc ggcgcgggca atcgcttcca gccgtggatg cccttccagc gcaaagcgtc
    27841 taccgagtac accctttgcc agaccgtcga tggcaagcgg aataaactgc cgcgaatcgt
    27901 aacgtagcaa cgcagacgca tcgcactcca gcacctgacg tagcgtggtg atcaggcgct
    27961 gaaaacgatc ctgatgacca atcccacgct gcaattcgat ggcgatattc gccagcacat
    28021 caacggaaaa actcatcttt gcctcactgt caatttgact atagatattg tcatatcgac
    28081 catttgattg atagtcattt tgactactca ttaatgagca taattttatt tatagagtaa
    28141 aaacaatcag ataaaaaact ggcacgtaat ctgcaattag caagacatct ttttagaaca
    28201 cgctgaataa attgaggttg ctatgtctat tgtggtgaaa aataacattc attgggttgg
    28261 tcaacgtgac tgggaagtgc gtgattttca cggtacggaa tataaaacgc tgcgcggcag
    28321 cagttacaac agctacctca tccgcgaaga aaaaaacgtg ctgatcgaca ccgtcgacca
    28381 taaattcagc cgcgaatttg tgcagaacct gcgtaatgaa atcgatctgg cggatatcga
    28441 ttacatcgtg attaaccatg cagaagagga ccacgctggg gcgctgaccg aactgatggc
    28501 gcaaattccc gatacgccga tttactgtac tgccaacgct atcgactcga taaatggtca
    28561 tcaccatcat ccggagtgga attttaatgt ggtgaaaact ggtgacacgc tggatatcgg
    28621 caacggcaaa cagctcattt ttgtcgaaac gccaatgctg cactggccgg acagcatgat
    28681 gacttacctg acaggcgacg cggtgctgtt cagtaacgat gctttcggtc agcactactg
    28741 cgacgagcat ctgttcaacg atgaagtgga tcagacggag cttttcgagc agtgccagcg
    28801 ttactacgcc aatatcctga cgccgttcag ccgcctggta acaccgaaaa ttaccgagat
    28861 cctgggcttt aacttaccag tcgatatgat agcgacctct cacggcgtgg tatggcgcga
    28921 taacccgacg caaattgtcg agctgtacct gaaatgggcg gctgattatc aggaagacag
    28981 aatcaccatt ttctacgaca ccatgtcgaa taacacccgc atgatggctg acgctatcgc
    29041 ccaggggatt gcggaaaccg acccacgcgt ggcggtgaaa attttcaacg tcgcccgaag
    29101 cgataaaaac gaaatcctga ccaatgtctt ccgctcaaaa ggcgtgctgg tcggcacttc
    29161 gacgatgaat aacgtgatga tgccgaaaat cgccgggctg gtggaggaga tgactggttt
    29221 acgcttccgt aacaaacgcg ccagtgcttt cggctctcac ggctggagcg gcggtgcggt
    29281 ggatcgtctt tccacgcgcc tgcaggatgc gggtttcgaa atgtcgctta gcctgaaagc
    29341 gaaatggcga ccagaccagg acgctctgga gttatgccgt gaacacggtc gcgaaatcgc
    29401 ccgtcagtgg gcgctcgcgc cgctgccgca gagcacggtg aataaggtag tcaaagaaga
    29461 aacctctgcc accacgacgg ctgacctcgg cccacggatg cagtgcaggg tctgccagtg
    29521 gatttacgat ccggcaaaag gcgagccaat gcaggacgtt gcgccaggaa cgccgtggag
    29581 tgaagtcccg gataacttcc tctgcccgga atgctccctc ggcaaagacg tctttgaaga
    29641 actggcatcg gaggcaaaat gagtaacggc attgtgatca tcggttcggg cttcgccgcc
    29701 cgccaactgg tgaaaaatat tcgcaaacag gacgccacta ttccattaac cctgattgcc
    29761 gccgacagca tggatgagta caacaaacct gacctcagcc atgttatcag tcaggggcaa
    29821 cgtgccgatg accttacccg ccagacggcg ggtgaatttg ccgagcagtt taatctgcac
    29881 ctgtttccac aaacctgggt gacggatatc gatgccgaag cccgtgtggt gaaaagccag
    29941 aataatcagt ggcaatacga caagctagta ctggcaaccg gtgccagtgc ctttgtcccg
    30001 cctgtgcctg ggcgtgagtt aatgctgacg ttaaatagtc agcaagagta tcgcgcctgt
    30061 gaaacgcaac tgcgggatgc ccgacgcgtg ttgattgttg gcggtggttt gattggtagc
    30121 gaactggcga tggatttttg tcgtgcaggc aaagcggtca cgctaatcga caacgctgcc
    30181 agtattctgg cgtcgttaat gccaccggaa gtaagcagcc gcttgcagca tcggttgacg
    30241 gagatgggcg ttcatctgct gttgaaatct cagttacagg ggctggaaaa aacggattct
    30301 ggcattctgg caacgctgga ccaccagcgt agtatcgaag tggatgcggt aattgccgcc
    30361 accggactgc gcccggaaac agccctggca cgacgcgccg ggctgacgat taatcgcggc
    30421 gtttgcgtcg atagttatct gcaaaccagt aatgacgata tttatgcgct gggcgattac
    30481 gcggaaatca acggtcaggt actgccattc ctgcagccga ttcaacttag cgcgatggtg
    30541 ctggcaaaaa atcttctcgg caataacacg ccgctgaaac taccgacgat gctggtgaaa
    30601 atcaaaacgc cggaattacc gctgcatctg gcaggcgaaa cccagcgtca ggatttacgc
    30661 tggcaaatta ataccgaacg ctagggaatg gtggctcgcg gtgttgacga tgctgaccag
    30721 cttcgcgcct ttgtggtcag tgaggatcgg atgaaagagg catttggatt gttgaaaaca
    30781 ttgccgatgt aggtgggcta ctgtgcctaa aatgtcggat gcgacgctag cgcgtcttat
    30841 ccgacctacg gggacgcatg tgtaggccgg ataaggcgtt tacgccgcat ccggcaatgg
    30901 tgtccaaatg caacacgttt tatccgttct ggacttcacc cgctaaccaa cgcgccgcag
    30961 caataacccc ctgccccaga gacaaaccgc catcacccgc cggtaaactc tgtggaaaga
    31021 gcaatgtgaa atcagcgaga taatgcgcca gacgtgcacg cagcaaacgg ttatgaataa
    31081 ccccgccgct aaataccagc gtagtgatac cacgcatcgt ggcctgctca cgcatcaacg
    31141 cggcaaaacc ctgcgccagc gcatcatgaa acgcccacgc gcgttgatta acctgtgcct
    31201 gccagttcag ccactgctgc cagaaagcgg cgagatccag ttgattgtcc accagcggca
    31261 tcgtcaccgg atgcgtcact ccgtggcatg aggctgcgag cgcctccaga gcacaagccg
    31321 cttcaccttc ataacttaac gtggctggcg cacagcccag tgccgccgcc actgcatcga
    31381 acaaccgccc acacgatgac gccagcggcg cgttaattcc acgctcaatg gcccgcgcca
    31441 gcacgctcca gttttgctgt tgcacacttg ctgtttcagg gtaattctgc cactccggca
    31501 caaagcgcag gcactgcgcc agcaggtttc gccacggctg cttcgctgcc aaatcgccac
    31561 ccggaagcgc cactgcaggc aagccgccca ggtgctcaca ttcgcgatag ttcacccgca
    31621 ggcactcgcc gccccacaaa gcgccgttct cccccatacc gataccgtcg agcgtcaaag
    31681 caatgacatc accgccatcc agcggccact gatgctctgc cagacacgcc gctgcatggg
    31741 catgatgatg cagtaccgtt tgcgtcggca gattcatttc acgcgcccac tggctggaga
    31801 catagcccgg atgcgcgtca tgcacaacgt atttcggggt aaaatcgtag atgttttgca
    31861 tcaggcgtaa cgcttcgcgc cactgcatct ggatgccatc gtcacttaaa tcgcccagat
    31921 gctgactcaa caccgcttgt tcaccgcgca ccaggcagaa ggtatttttc agatccgctc
    31981 cgagacacag cacaggcgga acatttttaa agcccggagg caaagccagc gcatccggca
    32041 catacccccg cgaacggcgc agcatttcgc cgctttcgcg caccaccgaa tcatccatcc
    32101 gctgcacgat gtcgcggtta tgtatcaaga atccgtcggc aatgccctgc aaatccgcca
    32161 gcgcctgttc gttgctgata gctggtggtt taccgctcag gttgccggag gtcatcacca
    32221 gcgggcattg cagttcctgt aacagcaaat gctggagcgg gttcgcaggc aacattaccc
    32281 cgacttcgtt aaggtcaggg gcgatatcat cacaaagctc aggaacgtat tttttatcca
    32341 ccagcacaat cggcgcggcg ggcgtggtaa gcaactggcg cgcagcgtct ggtaaaccgt
    32401 cagccactgg caacatgacc gccagcggtt tcgccgggcg atgtttgcgc gcccgaagtg
    32461 tcgccaccgc gttactgtta cgtgcatcgc aggcaagatg aaatccgcca atccctttga
    32521 tggcgacaat gttgcccatt tttaactgtg cgatagccgc ctgtaatgcc gcctcttgtt
    32581 ctgcatgttc accatgactt acccattcaa gatgcgggcc acactccggg caggccaccg
    32641 gctgggcgtg gaagcgacga tcgagcgggt cgcggtactc tttgtcacaa gccggacaca
    32701 acggaaacgc cgccatcacg gtaaacgggc ggtcgtaagg catggcgcga ataatggtga
    32761 aacgcgggcc gcagtgggta cagttgataa acggataacg ataacgccgt tcgcctgggg
    32821 tattcatttc ggcaaggcaa gcagggcaag tagcggcatc cgggacaatt tgcgtattca
    32881 tggcacctcc cgcgctctgg cggatggtga actcagtggg cagttgtgac cagatgtacg
    32941 gctcacgctc gacgctatca atacgcgcca gcggcgggca gtgctgatgc aattgaacaa
    33001 gaaacgtttc cgggtcttcc cgcagccgga cttctacgcc atcgccgtca ttacagacat
    33061 cgccgtgaag atttaattgc tgtgccagct gccagacaaa cggacgaaaa ccgacgccct
    33121 gcactttgcc acgaatacgc agttggacac cgcaagatgt gttttttgcc attgagttat
    33181 tcccgccatc atgaattgcg taacccgccc agccggacac gacagcgtcg catccggcag
    33241 tcacaggtcg gcgataccgc ccgctccgta ttctacgaat atttccggga attcctttga
    33301 tgccagaaca gttctgtaag atttttagaa catcagcgcc gtacggcggc gtttttctgc
    33361 actcagttgt tcaagtttat tacgatcgac acaaatcagc gcatgagtcg ggcaagccgc
    33421 catacacgcc gggccgtctt cacgatggtt gcacaggtca catttattgg cttcggcttt
    33481 gtcagcccgt acattcagcc ccgcgccgct gttgcgaatc accggacgta ccaccacttc
    33541 catcgcgcca tacgggcaag ccacaacgca ggttttgcaa ccaatgcaac gttcctgcat
    33601 cacatgaaca aaccctttat cacggctgat agcaccattc gggcagacgt tagcgcacgg
    33661 tgcatcttca cactgacggc aaactgtcgc cgtggaaatg ttcacacctt taatgacatg
    33721 gatacgcggt aaaaatgttt ccggggtcag cgatgcacag tcctgatttt cctgatgaga
    33781 aaccacgcac gccacttcac aggtacggca accaatacat ttactcgcgt cagcaatgat
    33841 gaaacggttc atcaaattct ccagcaatga cagttaatgc gccgatacat tcacaaatca
    33901 tgccagtttt taattcactg ttatttaagg aaattaattt ctgtaatgca ggaaaaaaga
    33961 tgtcatcgac actagtgacg atgacatgtg atgacaatgt ttatcgcgaa agagcaatga
    34021 gtgagtcgcg gtggatcagt tttccgctga aggttttcgg cggtgagaaa tccccgccat
    34081 cgagcataaa aatcagccgt ccaataattt cctgaatcat ctcagtcacc ggaattttta
    34141 cgctggagag cgccggaacg atataggggg caattgcgat atcatcgaat ccgataactg
    34201 acacctgctc tggcaccgct acgccgcgct cgtgcaacgc tttcatcgca cctatcgcca
    34261 tatcgtcgtt actggcaact aacgcgctaa atttagcccc acgttcgagc aacgtttcta
    34321 ccccttcggc cccgctggcg ggcgtccatt taccgttagc gataagtttt tcattgagcg
    34381 caataccatg ctgcgccagc gcgtctttat acccagcaag acgttcaatg ctggtggggg
    34441 aatccatcga gccggtaagg aaagcaatct cctgatgtcc ggcgtttatc aactctgcca
    34501 cggcattaaa gctggtctgt ttatgatcgc accagacgct atggctgctg tttttgcgca
    34561 ggcggcgatt aagcaccatt atcggctgac tgtgcgcgtc aatgatgtca tcgatctcat
    34621 ccacgcttaa aaaacgcggg taaatcataa tggcgtcgca gcgcaaatcc agcagatact
    34681 gaatcgcctg gcgctcttct tcggcgctgt gtttaccatc tgccaatagc aactgccgcc
    34741 ctttctcttc cgccattcgc gcggcatgaa agagtaattc actaaaataa atgccgtggt
    34801 aaagcgtgtt ggtcactacc agccccagcg tctgagtact cttcgccgac agattgcgcg
    34861 ccagcaagtt tgggcggtaa ccgctctctt ctaccgcctg aaacacgcga tctttagtct
    34921 cctggctgac gtagccatta cctgaaagca cgcgggaaac ggtcgctttt gaaaccccgg
    34981 cgcgcttcgc cacttccagc atcgtcgtca tcattttcat ccctttacac gcaatcaacg
    35041 cagtgtactg caccgtttgc cgattgtcct tgcgcaatcg gcgggaaaaa tattcaggtg
    35101 accggtttca caaatataaa aaatgaataa ttaactctct tgcttattta gtgacaacta
    35161 ttcatgattt tgtgaaaccg gtttcctaat tccgtttcag catcggcatt tttccgttac
    35221 gtcgactgat aacaactaca tctaccctac tgataacagg ataaaatccg atggccaaaa
    35281 attatgcggc gctggcacgc tcggagatag cggcactggg cggcgttgat aacatctcgg
    35341 cggtcacgca ctgtatgacg cgtttgcgct ttgttatcaa agatgaccag cttatcgaca
    35401 gcccgacgtt aaaaaccatc cccggcgtgc tcggcgtggt acgtagtgac aaccagtgtc
    35461 aggtgattat cggcaatacc gtttcacaag cctttcagga agtcgtcagc ctgctgccgg
    35521 gagatatgca gcccgcacag cccgtgggta aacccaaact cacgccgcgt cgcattggtg
    35581 cggggatcct cgatgcgctg atcggcacca tgtcaccgct gatcccggcg attatcggcg
    35641 gatcgatggt caaactgctg gcgatgatcc tcgagatgag cggcgtgctg acaaaaggat
    35701 cgccgacctt aaccattctg aatgtgattg gtgacggtgc tttcttcttc ctgccgctga
    35761 tggtcgcggc atctgccgcc atcaaattta aaaccaatat gtcgctggcg attgccatcg
    35821 cgggtgtgct ggtacatccg agttttattg aactgatggc gaaagcggcc cagggtgaac
    35881 atgtcgaatt tgccctgatt ccggtcaccg cggtgaaata cacctacaca gtgatcccgg
    35941 cgctggtgat gacctggtgc ctgtcatata tcgaacgctg ggtggatcgc attacaccgg
    36001 cggtgaccaa aaacttcctc aagccaatgc tgattgtgtt gattgccgca ccgctggcaa
    36061 tcctgctgat tggcccgatt ggtatctgga tcggtagcgc catttcggcg ctggtttaca
    36121 gcattcatgg ttatctgggc tggctttcag tcgccattat gggcgcgctg tggcctctgc
    36181 tggtaatgac cgggatgcac cgcgtcttta cgccaaccat cattcagacc attgccgaaa
    36241 ccggcaaaga agggatggtc atgccgtcag agatcggcgc taacctgtcg ctgggcggtt
    36301 catcactggc ggtggcgtgg aaaacgaaaa acccggaact gcgccagacg gcgctggctg
    36361 cggcggcatc agccattatg gcggggattt ccgaaccggc gttatacggc gtggcgatcc
    36421 gcctgaaacg tccgcttatc gccagtctta tcagcggttt tatttgcggc gcggttgccg
    36481 gtatggcggg gcttgccagc cactcaatgg cagcgccggg gctatttacc agcgtgcaat
    36541 tcttcgatcc ggcgaatcca atgagcatcg tctgggtgtt cgcggtcatg gcgctggcgg
    36601 tggtgctgtc gtttatcctc acactgttgc tcggctttga ggatattcct gttgaggaag
    36661 cggctgccca ggcgcgaaag catcagagcg tacaaccgac cgtcgccaaa gaagtaagtc
    36721 ttaattgagg atgaaaatgt cagtatttcc agaaggtttt ttatggggcg gcgcgcttgc
    36781 cgccaaccag tctgaaggtg cgttccgtga aggcggaaaa gggctgacca ccgtcgatat
    36841 gatcccacac ggcgagcatc gaatggcggt gaaactgggg ctggaaaaac gttttcagtt
    36901 gcgcgatgac gagttttatc ccagccatga ggcgacggat ttttatcatc gttataaaga
    36961 agatatcgcc ctgatggcag agatgggatt caaggttttc cgtacctcaa ttgcctggag
    37021 ccgtctcttt ccgcagggcg atgaaatcac gcccaatcag cagggcattg ctttttatcg
    37081 ttctgtcttt gaagagtgta aaaagtacgg tatcgaaccg ctggtcacgt tgtgccactt
    37141 cgatgtgccg atgcatctgg tcaccgaata tggctcctgg cgtaaccgca agctggtgga
    37201 gtttttcagc cgctacgcca gaacctgctt tgaagcattt gatggtctgg tgaaatactg
    37261 gctaaccttc aatgaaatca acattatgtt gcatagcccg ttctccggcg cgggtctggt
    37321 gtttgaagaa ggtgaaaatc aggatcaggt gaaatatcag gccgcgcatc accagctggt
    37381 tgccagtgcg ctagccgcca aaatcgccca tgaggttaac ccgcaaaatc aggtgggctg
    37441 tatgctggcg ggcggcaact tctaccctta cagctgcaaa ccggaagatg tctgggcggc
    37501 gctggagaaa gatcgggaaa acctgttttt tatcgatgtg caggcgcgcg gcgcgtatcc
    37561 ggcttactct gcccgcgtat ttcgcgaaaa aggggtaacc atagataaag cgctgggcga
    37621 tgatgagatc ctgaaaaaca ccgtcgattt tgtctctttc agctattacg cctcgcgctg
    37681 cgcctcggcg gagatgaacg ccaacaacag cagtgcggcg aacgtggtga aatcgctgcg
    37741 taacccgtat ctgcaagtga gcgactgggg ctggggaatt gatccactcg gtctgcgtat
    37801 caccatgaat atgatgtacg atcgttatca gaagccgctg tttctggtgg aaaacggcct
    37861 cggcgcaaaa gatgaacttg ctgccaatgg cgagattaac gatgactatc gcatcagcta
    37921 tttacgcgaa catatccgcg caatgggcga agcgattgca gatggcattc cgctgatggg
    37981 ctacaccaca tggggctgta ttgatttagt ttccgcctct acgggtgaaa tgagcaaacg
    38041 ctacggtttt gtctacgtag accgtgacga cgcaggcaac ggcacgctga cgcgcacacg
    38101 taagaaatcg ttctggtggt ataaaaaagt gattgccagt aatggggaag atttagagta
    38161 agtaacagtg ccggatgcgg cgtgaacgcc ttatccggcc tacatgtcgc acgaaccagg
    38221 aaggcccgat aagcgcagcg catcgggcaa tttagcattt ggcatcagag ctggttcacc
    38281 ccatcaagaa catccctgtc ctgattcctt aatgaaaaac tactcttctt ccaccgctaa
    38341 ctgcgcgaag ccgccattcc cttcccagcc ttccagtcgt tgataaacgg tttctaccgc
    38401 atctttaatc ggctgggtca tcgggtagta aaagccgacg atatccggct gtatgccgag
    38461 gaaaatcact tcgccaatat cttctttcaa ctggtcgata agatagttca gaggcatgtt
    38521 atgggtagtc atcataaaca tctcggcgat atcatccggg tcgatgatgc ggatctcgcc
    38581 ggggtttagc cccatatccg tggcgtcgac aatcagtaac cgcgtagggc gcagttcacg
    38641 gatggcgacg atgtcgtttt ccggtgcact accgccgtca atcaccaccc agttaccttt
    38701 cggcgcggcg gcgcactttt ccgccagcag cggacctgcg ccatcatcgc ccatcatgct
    38761 attgccaaca cagagtaaaa cgtcagtcac gcaatctcct caccatcaaa tagatggcgt
    38821 tttcctgatg aatatcatgc agcatactta acagcattgt actccattcc tgctgctgcg
    38881 aagtttgcgc tgcccgggct ttatcgaatg cgttggccag catcggcaca tggttaatgt
    38941 cgatgacgat ctcaccgtac ttcggtacgc cttccatttt gcggcgggct tcactgcccg
    39001 cctccagagt ggcaatccat gccagatatt catcccacgg gcaggtgagc gccgcttcca
    39061 ggcaatcgat aaccccaagg tggtgaccaa tcgccaggct gtaatagacc acctgctgcg
    39121 cctcggcggg cgtggcatcg ttctcatcaa taaatttacg gctcagttga ctgaacacca
    39181 ccttttcact catcggatac gcacctcttc tacaacatga ttcagatggc tgacaatctc
    39241 gttcagacgc ggatcgtttt ccgcttccag ccagcgtgca acctgttctt cgccctgccc
    39301 taactgtgtc aggaagtcat cggcaatctg acgaccgtaa cgataacccg ccaggcggcg
    39361 tgcttcgcga tccactttca cgcgcagcgg ctgcaccata tcgccatgca ggatctccgc
    39421 cggttgttca tccagttcac ccggcccacg ggcgtgaatt ttctgctcca gcaggccgag
    39481 cgccattgca aagccgtaca gcgtggcggc aggcgttggc gggcagccag ggatataaac
    39541 atccacaggg acaattttat ccgtaccgcc ccacacgcag tagagatcgt ggaagatccc
    39601 gccgctgtta ccgcaggcac cgtaggagat acaaattttc gggtccggcg cggactgcca
    39661 cgcacgcagc gcaggggatc gcattgcacg ggtgaccgcg ccggtaaaca gtaaaatatc
    39721 cgcatgacgc ggtgaaggaa cgactttaat gccgaagcgt tctgcatcaa acagcggcga
    39781 taaagtggcg aaaatttcga tttcgcagcc gttgcagccg ccgcagtcca cgcggtaaac
    39841 ataggcagaa cgtttgattt ttttcagtaa cgacgccttc atgctggcga tggattcatc
    39901 caccgtcatc gggaccggaa tgccgttggc gtcacggggg cctaataaat tgctcatcag
    39961 atggcctctt tcatatggcg agtcagttca atacggtcgg acggcaccag gcatttctgg
    40021 cgcttacatt ccgggcaagt ctcaaagctt tcgcggtggt tttccgcgcg gctgtcgccg
    40081 ttgtgcttaa gcagcgcaat ggcgtagtcg atctctttct ggacggcgaa aggacgattg
    40141 cagacgcgac agttgcacag cgcgaagcga gactgttgca ggaagtcttc tttcttccac
    40201 accgccagtt cgtactcttg cgacagctta atcgccgccg tcgggcaaac ttcttcgcag
    40261 cgtccacaga agatgcagcg cccgagatta aactcccagg ccagctctcc tgtggcgagg
    40321 tcagtttcaa ccgttaaggc gtttgacggg caggcattga cgcaggccgc gcagccgatg
    40381 cactgctgcg ggttctgctc tggcttacca cggaagtttt tatcaaccgc aatcggctcc
    40441 agcggataag acgaggtcgc cgtaccggtt ttgataactt ttttgataaa ggtaaacatg
    40501 gcgattcctt atttcagcgg cgagttttta cgctcaatgc tgtagcgctc gagttctttg
    40561 tacggcacca ctttgctctt cttcttacgt acatcgacca cagtcatgcg gtcggtacag
    40621 gagtagcaag ggtccaggct accgataatc agcggcgcat cggaaacagt gttgccacgc
    40681 agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg cacggcagcg ccagcggtac
    40741 agcttctggt tgtcgccagt catgctccag tggatatcat caccgcgcgg cgcttcggca
    40801 aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt ccaccatcag cgggccaccc
    40861 ggcaggttat ccagaccgta gtcgatcatg ttcagcgcgg tatagacttc gttgatacgc
    40921 actttcagac gcgaaataac gtcgcagccc tgctcgctgt ggacttccat tggcagcagg
    40981 ccataaccaa caaacgggtg atcagcgcgg gtatcgcgag cgtgtccgct ggcgcggacc
    41041 atcgggccaa cgttactgaa atcgcgggca atttccgggt ccagacgacc aatgccgacg
    41101 gtgcgctgtt ccatgttcgg tgtgctcagc agcacatcca ccagctcctg cacttcacga
    41161 cgcatctgtt gcgccagctg gcgggtctgg atcatgtcat ctttcaacag atcgcgacga
    41221 atcccgccga tcaggttcag gccgtaggtt ttacgcgcac cggtgaggat ctcggccatt
    41281 ttcatggagg tttcacgcac gcggaagaat tgcataaagc cggagtcgaa gccggtaaag
    41341 tggcaggcga ggccgaggtt gagcagatgc gagtgcaggc gttccacctc cagcagaatg
    41401 gcgcggatca tctgcgcacg ttctggcacc tgaataccca tcgcgttttc caccgacgtg
    41461 gtgtaggcgg tgctgtgggc gaagccgcag atcccgcaca cacggtcaga gaggaaggtc
    41521 acttcgttat aacccatgcg ggtttccgcc agtttttcca taccgcgatg gacatagaac
    41581 aagcggtagt ctgcgtcgat aatgttttcg ccatcgacga acagacggaa gtggcccggt
    41641 tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt tgtttttctt gtcgcccagt
    41701 tcgttgatga actcgtaggt ttcagcatcg gtggtcggtg ccggacgctg acgataatcc
    41761 atgctgtctt tacgcagcgg ataaagttca tccggccagt catccggcag caccagacgg
    41821 cgttcatccg gcagaccaac cggaatcaaa ccgtacatat cgcgcacttc gcgctcgccc
    41881 cacaccgccg ccggaacgcg cggcgtcacg gacggatact ccggtttgtt ggcatcaact
    41941 tcgacgcgaa ccgttaccca gcacttagtc cccttctcca tcgacagcac gtaataaacg
    42001 gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaaa gcaccgacag ccagcccccc
    42061 tgcttgtagt agagaaactc caccacttcc ggcagatagt tcacctttac ggtgacagtc
    42121 agctgctctt tggtctgcca ggcgtggtcc agcacgacgc ccggaaatgc ctcattcagc
    42181 gcggcgagat aatgttgacc taatttttct tcagacatgc tcaaactctc tttaatcacg
    42241 ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa cccagcccag gtaatgcgcg
    42301 aagtaatatc aagacgcaga cgggccatgc tgttttcgaa cagcgcgata accaggacgc
    42361 cgaccaccag ttttacgatc gcaatcacca gcgccagcag cagtccaccg gcggtgaagg
    42421 tttccatttg tccccacgga ataaacaccc cgacgaacat ctgcaacacc accagctgtt
    42481 tcaggctgat accccatttc atgacgccaa agccgctgcc gctatactca gagagcggac
    42541 cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg cagtttgccc atttcgataa
    42601 aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg gatgctttgg ctcagcggcc
    42661 agtgataaac ggtgtcggta atgttgctga tgttggtgga accggcaacc tgtgcggcaa
    42721 cccacagacc aagcagcaac atcggttcga ccagcacgcc aagcatcgct tcacggctcg
    42781 caccgatagc ggtaaacggg ctaccggtat ccagaccgga aatggcaaag aagaaacgcg
    42841 cgatggcgaa gagatacagt aaggtgatca aatcgcccag ttgcggcagc ggagaaccga
    42901 cggtcaccac cggcagcgca gtagcgatag tcagcatgac gcccaccatc acatacggcg
    42961 tcaggcggaa cacccagcca gaggcatccg ggccaacgct ctggcggccc agcagtttga
    43021 taatgtcgcg atactcctgc aacacgcccg gcccgcgacg gttatgcaga cgggcgcgcg
    43081 ccacgcgggt aataccggag agcagcggcg caacggcaaa taacaccagc gcctgaatta
    43141 acggatataa aacactcatt ctcaggctcc tcgtgaaaca ataatcacca ccagtaccgc
    43201 cagttcaacc agcgccatcc ggcggaacag caacgcacta ccttcgcact gccagcccgg
    43261 caccagcgac accggattca gccatttgcg cagtttcagc actggcgcaa acgcctgttt
    43321 aaccggcatg gcaaaaccgt gagcggtaat caccattgat ttttcatggt cgtagccgca
    43381 cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg cctttgcaaa tcgccataat
    43441 gatgaatggc agcagcgggc aggcaatcag cagcaatgtg atcattggtt gagaaacggt
    43501 ggtgttagca ggctccagag gcagaggtac ggcagcagag agcatcggca gcagccacgg
    43561 cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc actacgctta cgctcatcag
    43621 gagcggcgca caggtggcgt tttcggcttc tttggtacgc ggcgcgccga ggaacgtgac
    43681 gccatagact ttcgccatac acatcaccgc cagcgcaccg gtaattgcca gacccacagc
    43741 gagcagcggc cccagcagac gggcaacaaa cgcgccacta ttgctcagtt tgaaaaagga
    43801 ttgatagata acccattccc cggcaaaccc gttcagcggc ggcagcgcag ccattgccat
    43861 caacccgact aacatggcga tggagataac cggcattttc ttgccaatac caccgagttt
    43921 ttcgatatcg cgatgaccgg tacggaacca gacgctcccc gccccgagga acagtacgct
    43981 tttgaacagg ctatggttaa gcagatggta cagaccaccg accaggccaa gagcaatcag
    44041 cgccggttgt tcgagcgcga tacccgttac gccagcgccc agccccagca ggataatgcc
    44101 gatattttcc agggtgtggt aagccagcag gcgctggatg ttgtgctcca tcagcgcata
    44161 cagaccaccg acaaacgcgg tgatcatgcc gagcaccagc agcacgatcc cccaccacag
    44221 cggtgcatta ccgcccagca gtgacaggat taaaatgccc agcaggccaa ttttcatgac
    44281 taccgtagaa aacaacgcgg cagctggcgc agaggcgtta gcatgtgcct gcggcaccca
    44341 gccgtgcagc ggaataatcc cggcgagcag gccaaagcca atcacaccga gcagccagat
    44401 atcggaaccg agcggcagct gttgcatacg catatccagc aggcgcagat ccagcgtgcc
    44461 ataacgctgc cacagcagcc agcaagcaat cgccagcagc agagtgccaa gacgccccag
    44521 cgcaaaccac agtttgccct ctttgctgtt gctggtgagg aacaccgcac acaaggccat
    44581 gacttcggcc attaccacga acatgccgag gttgctggcg atgacggcgc agacggcagc
    44641 agccatcaac atattgatct gcaagccgtt gcacttcacc tgcgcgtggc gatgccagtc
    44701 aatgttgtag aggctgacaa acagaccgca cagaccgagc gtaatcagcc aaatcgcgtt
    44761 aagcggggag atttgcacat cgtagcttac cagcgacagc gcaccgctca cgccaaccgc
    44821 gccagtcagc acagtgaagc ccgcggctgc cgtatacagg ctaccaaccg cgccgccaat
    44881 tccggctatc cagccactta gcgtcttttg aaaagaaaag agaaatgcca gaacagcggc
    44941 ggcgacaaac cacaccacgc cgctattgat cagggaaatt gcgctcattt agcctctcca
    45001 ctttgagcct gctgaaacaa ggtgagatcg ccaaagtccg tattaaaagt cagctcacgc
    45061 ttacgtttgc tgacgcgggc gatatcggtg ttatccacca gatgcagggc tttggtcggg
    45121 cacatccgca cgcaggccgg accttgttca tcaaagctac aaaggtcaca tttgacggcg
    45181 atcgcgcgaa tacccggcac ccagtcaagc agcgtactga tacgcgccgg agcaggcggt
    45241 gccggtggcg ctttcggggt attggcattt gcaggaatat ccagcggacg gctgccggaa
    45301 aattcaattg cgccaaacgg gcaggcgatg ccgcacagct tgcagcttac gcacaggctt
    45361 tcattcaact gcacggcccc atcaacgcgg gtgatgacgt taaccgggca gaccaccgcg
    45421 cagggtgcat cttcacagtg gtgacagagc tgcggcgcag attctttttc attcagcatc
    45481 actctcaggc gcggcattga ttgcaggccg tgctggcgat gcgtctctga acaggctgcc
    45541 tcacaagtgt ggcagccgat acagagcgtg gagtcagcaa ttacaaaacg attcaccagg
    45601 cattcctcag gtgattgtca tttttgacga aaacatgccg atgaaatgtc attttcgaca
    45661 ctcatcgaca cgcccatccc caaacaggcg taacgcctgc aaaacgggca aagcctcagc
    45721 tcatgctgcc gggctttgtc cctttaccag ttggcttaaa ttcaccggca cattgttttc
    45781 aatggcggtg tataagctgt cgtaaacgcc agcgattttt tcgagatagt gttccagaac
    45841 ctgttcgcga tcgcggatgt tgaccttgcc atcaatcatg ccgtcaatgc tcagttgcgc
    45901 ctgcgcaatc cgctgtttgt cttcgccctc ttttgtttcg acgtaatact cgatggtgtg
    45961 gctgttaaag ccatccgcca gggtgacgta aatacgaaaa tgttcaaaaa ggacgaaaca
    46021 gagttctttg tccggcgcgc agctcatggc gtgatgcagg cgcgctttcg ataacgtgat
    46081 cccctgaacc agcgaattgc agtagatgtg ccactggtcc tgtaggcgac gatgccgctg
    46141 tgcgatgtaa tcggctttct cgcttatttc ccaaatagtc attgtcaggt tacccgttta
    46201 acagagatgc cagctttaag cattttctgt gccaattttt aatttattgt tattaaaacg
    46261 atttttaagc taaagatgaa tttcgtcgcc gtgtcgacgt gtcatttcga catcatcgac
    46321 attattcacc gcagggataa tcaacactgg cacaattatt gcttgtagct ggcaatagtt
    46381 aatgggaggc gatatgcacg aaataaccct ctgccaacgg gcactggaat tgatcgaaca
    46441 gcaggccgca aaacacggcg caaaacgcgt aactggggtc tggctcaaaa ttggcgcatt
    46501 ttcttgtgtc gaaaccagct ctcttgcctt ttgttttgat ctggtttgcc gcggcagcgt
    46561 ggcggaaggt tgtaaactgc acctcgaaga acaagaggtc gaatgctggt gtgaaacatg
    46621 ccaacagtat gtgacgctat tgacccagcg cgtccgccgc tgtccacagt gtcatggtga
    46681 catgctacag attgtggcag acgacggttt acagattcgg cggatagaaa tagaccagga
    46741 gtgagcgatg tgtacaacat gcggttgcgg tgaaggcaac ctgtatatcg agggtgatga
    46801 acataaccct cattccgcgt ttcgtagcgc gccatttgcc ccggcggcac gcccgaagat
    46861 gaaaatcacc ggcattaaag cgcctgaatt tacccccagc cagactgaag aaggcgacct
    46921 gcattacggt catggcgaag cgggcactca cgcaccgggc atgagccagc gtcggatgct
    46981 ggaagtcgaa attgacgtgc tggacaaaaa caaccgtctg gctgaacgca accgcgcgcg
    47041 ctttgctgcc cgcaagcaac tggtgctcaa cctggtttcc agcccaggtt ccggtaaaac
    47101 caccctgctg acggaaaccc taatgcgcct gaaagacagc gttccgtgcg cggttattga
    47161 aggtgaccag caaaccgtga acgatgccgc acgcattcgc gctaccggca caccagcgat
    47221 tcaggtgaac acaggtaaag gctgccatct tgacgcacag atgattgccg acgccgcacc
    47281 gcgtctgcca ctggacgata acggtattct gtttatcgaa aacgttggca acctcgtatg
    47341 cccggccagc ttcgatctcg gtgaaaaaca caaagtggcg gtgctttccg ttaccgaagg
    47401 tgaagacaaa ccgctgaaat atccgcatat gtttgccgcc gcctcgctga tgctgctcaa
    47461 caaagttgac ctgttgccgt atctcaactt tgacgttgag aagtgcatcg cctgcgcccg
    47521 cgaagtcaat ccagaaattg aaatcatcct tatttccgcc accagcggtg aagggatgga
    47581 ccagtggctg aactggctgg agacacagcg atgtgcatag gcgttcccgg ccagatccgc
    47641 accattgacg gcaaccaggc aaaagtcgac gtctgcggca ttcagcgcga tgtcgattta
    47701 acgttagtcg gcagctgcga tgaaaacggt cagccgcgcg tgggccagtg ggtactggta
    47761 cacgttggct ttgccatgag cgtaattact gaagccgaag cacgcgacac tctcgacgcc
    47821 ttacaaaaca tgtttgacgt tgagccggat gtcggcgcgc tgttgtatgg cgaggaaaaa
    47881 taatgcgttt tgttgatgaa tatcgcgcgc cggaacaggt gatgcagtta attgagcatc
    47941 tgcgcgaacg tgcttcacat ctctcttaca ccgccgaacg ccctctgcgg attatggaag
    48001 tgtgcggtgg tcatacccac gctattttta aattcggcct cgaccagtta ctgcctgaaa
    48061 acgttgagtt tatccacggt ccgggttgcc cggtgtgcgt actgccgatg ggtagaatcg
    48121 acacctgcgt ggagattgcc agccatccgg aagtcatctt ctgtaccttt ggcgatgcca
    48181 tgcgcgtacc agggaaacag ggatcgctgt tgcaggcaaa agcacgcggt gccgatgtgc
    48241 gcatcgttta ctcgccgatg gatgcgttga aactggcgca ggagaatcca acccgcaaag
    48301 tggtgttctt cggcttaggt tttgaaacca ccatgccgac caccgccatc actctgcaac
    48361 aggcgaaagc gcgtgatgtg cagaattttt acttcttctg ccagcacatt acgcttatcc
    48421 cgacgctgcg cagtttgctg gaacagccgg ataacggtat cgacgcgttc ctcgcgccgg
    48481 gccacgttag tatggttatc ggcactgatg cctataattt tatcgccagc gattttcatc
    48541 gtccgctggt ggtcgctggt ttcgaacccc ttgatctact gcaaggcgtg gtgatgctgg
    48601 tggagcagaa aatagcggcc cacagcaagg tagagaatca gtatcgtcgg gtggtaccgg
    48661 atgccggtaa cctgctggcg caacaggcga ttgccgatgt gttctgtgtc aacggcgaca
    48721 gcgaatggcg cggcttaggc gtgattgaat cttctggcgt gcacctgacg ccggattatc
    48781 aacgattcga tgccgaagca catttccgcc cggcaccgca gcaggtctgc gatgacccgc
    48841 gcgcgcgttg tggtgaggta ttaacgggca aatgtaagcc gcatcaatgc ccgctgtttg
    48901 gtaacacctg taatcctcaa accgcatttg gtgcactgat ggtctcctcc gaaggagcgt
    48961 gcgccgcgtg gtatcagtat cgtcagcagg agagtgaagc gtgaataata tccaactcgc
    49021 ccacggcagc ggtggccagg cgatgcagca attaatcaac agcctgttta tggaagcctt
    49081 tgccaacccg tggctggcag agcaggaaga ccaggcacgt cttgatctgg cgcagctggt
    49141 agcagaaggc gatcgtctgg cgttctccac cgacagttac gtcattgacc cgctgttctt
    49201 tcctggcggt aatatcggca agctggcgat ttgcggcacc gcgaatgacg ttgcggtcag
    49261 tggcgctatt ccgtgctatc tctcctgtgg ctttatcctc gaagaaggat tgccgatgga
    49321 gacactgaaa gccgtagtga ccagcatggc agaaaccgcc cgcacggcag gcattgccat
    49381 cgttactggc gatactaaag tggtgcagcg cggcgcggca gataaactgt ttatcaacac
    49441 cgctggcatg ggcgcaattc cggcgaatat tcactggggc gcacagacgc taaccgcagg
    49501 cgatgtattg ctggttagcg gtacactcgg cgaccacggg gcgactatcc ttaacctgcg
    49561 tgaacaactg gggctggatg gtgaactggt cagcgactgc gcggtgctga cgccgcttat
    49621 tcagacgctg cgtgacattc ccggcgtgaa agcgctgcgt gatgccaccc gtggtggtgt
    49681 aaacgcggtg gttcatgagt tcgcggcagc ctgcggttgt ggtattgaaa tttctgaatc
    49741 agcactgccg gttaaacctg ctgtgcgcgg cgtttgcgaa ctgctgggac tggacgccct
    49801 taactttgcc aacgaaggca aactagtaat agctgttgaa cgcaacgcag cagagcaagt
    49861 gctggcagcg ttacattccc atccactggg gaaagacgcg gcgctaattg gtgaagtggt
    49921 ggaacgtaaa ggtgttcgtc ttgccggtct gtatggtgtg aaacgaaccc tcgatttacc
    49981 acacgccgaa ccgcttccgc gtatatgcta ataaaattct aaatctccta tagttagtca
    50041 atgacctttt gcaccgcttt gcggtgcttt cctggaagaa caaaatgtca tatacaccga
    50101 tgagtgatct cggacagcaa gggttgttcg acatcactcg gacactattg cagcagcccg
    50161 atctggcctc gctgtgtgag gctctttcgc aactggtaaa gcgttctgcg ctcgccgaca
    50221 acgcggctat tgtgttgtgg caagcgcaga ctcaacgtgc gtcttattac gcatcgcgtg
    50281 aaaaagacac ccccattaaa tatgaagacg aaactgttct ggcacacggt ccggtacgcc
    50341 gcattttgtc gcgccctgat acgctgcatt gcagttacga agaattttgt gaaacctggc
    50401 cgcagctggt cgctggtggg ctatacccct aatttggtca ctattgcctg atgccactgg
    50461 cggcggaagg gcatattttt ggtggctgtg aatttattcg ttatgacgat cgtccctgga
    50521 gcgaaaaaga gttcaatcgt ctgcaaacat ttacgcagat cgtttctgtc gtcaccgaac
    50581 aaatccagag ccgcgtcgtc aacaatgtcg actatgagtt gttatgccgg gagcgcgata
    50641 acttccgcat cctggttgcc attaccaacg cggtgctttc ccgcctggat atggacgaac
    50701 tggtcagcga agtcgccaaa gaaatccatt actatttcga cattgacgat atcagtatcg
    50761 tcttacgcag ccaccgtaaa aacaaactca acatctactc cactcactat cttgataaac
    50821 aacatcccgc ccacgaacag agcgaagtcg atgaagccgg aaccctcacc gaacgcgtgt
    50881 tcaaaagtaa agagatgctg ctgatcaatc tccacgagcg ggacgattta gccccctatg
    50941 aacgcatgtt gttcgacacc tggggcaacc agattcaaac cttgtgcctg ttaccgctga
    51001 tgtctggcga caccatgctg ggcgtgctga aactggcgca atgcgaagag aaagtgttta
    51061 ccactaccaa tctgaatttg ctgcgccaga ttgccgaacg tgtggcaatc gctgtcgata
    51121 acgccctcgc ctatcaggaa atccatcgtc tgaaagaacg gctggttgat gaaaacctcg
    51181 ccctgaccga gcagctcaac aatgttgata gtgaatttgg cgagattatt ggccgcagcg
    51241 aagccatgta cagcgtgctt aaacaagttg aaatggtggc gcaaagtgac agtaccgtgc
    51301 tgatcctcgg tgaaactggc acgggtaaag agctgattgc ccgcgcgatc cataatctca
    51361 gtgggcgtaa taatcgccgc atggtcaaaa tgaactgcgc ggcgatgcct gccggattgc
    51421 tggaaagcga tctgtttggt catgagcgtg gggcttttac cggtgccagc gcccagcgca
    51481 ttggtcgttt tgaactggcg gataaaagct ccctgttcct cgacgaagtg ggcgatatgc
    51541 cactggagtt acagccgaag ttgctgcgtg tattgcagga acaggagttt gaacgtctcg
    51601 gcagcaacaa aatcattcag acggacgtac gtttaattgc cgcgactaac cgcgatctga
    51661 aaaaaatggt cgccgaccga gagttccgta gcgatctcta ttaccgcctg aacgtattcc
    51721 cgattcacct gccgccacta cgcgagcgtc cggaagatat tccgctgctg gcgaaagcct
    51781 ttaccttcaa aattgcccgt cgtctggggc gcaatatcga cagcattcca gccgagacgc
    51841 tgcgcacctt gagcaacatg gagtggccgg gtaacgtacg cgaactggaa aacgtcattg
    51901 agcgcgcagt attgctaaca cgcggcaacg tgctgcagct gtcattgcca gatattgctt
    51961 taccggagcc tgaaacgccg cctgccgcaa cggttgtcgc tcaggagggc gaagatgaat
    52021 atcagttgat tgttcgcgtg ctgaaagaaa ccaacggcgt ggttgccggg cctaaaggcg
    52081 ctgcgcaacg tctggggctg aaacgcacga ccctgctgtc acggatgaag cggctgggaa
    52141 ttgataaatc ggcattgatt taactgcaaa ttgccggaca gatctgcctg tccggcatac
    52201 tattcacgag gttttttcgg acgatatttt tccggcagtt ctggcaccgg acgcttgtca
    52261 tcgatgagat gacgcacggt taagatcgga tgacgccaca gcattctcgg cccggcccaa
    52321 cgcataatct gtttcatctc ttcacgcttt gcaggctggt aacagtgcac cggacactgc
    52381 ttacaggctg gtttctcttc gccgaacaca catttatcca gccgcttttg cgcgtaaaca
    52441 aacaacgcct cgtaatgctc cggctccgct gacgcctgcg ggcatttcgc ttgataaaga
    52501 tcgatcattt ttttaatcgt cagtttttca cgagagatac gcttgccgga catgctgcct
    52561 ccatactctt aaggtgtatt tatattacaa cttaatttga aaggggacta tggctctgaa
    52621 ggaggaggtt tattcaaccg acgcaatttt gcgtacttgc ttcataagca tcacgcaaaa
    52681 gctgcaaaac agcatctttc ccggaaccag catcaagaac tcgccgttcg cttcttcccc
    52741 tgaaatgatt aactccggta tcatgtgcgc cttatgtgat tacaacgaaa ataaaaacca
    52801 tcacacccca tttaatatca gggaaccgga cataacccca tgagtgcaat agaaaatttc
    52861 gacgcccata cgcccatgat gcagcagtat ctcaggctga aagcccagca tcccgagatc
    52921 ctgctgtttt accggatggg tgatttttat gaactgtttt atgacgacgc aaaacgcgcg
    52981 tcgcaactgc tggatatttc actgaccaaa cgcggtgctt cggcgggaga gccgatcccg
    53041 atggcgggga ttccctacca tgcggtggaa aactatctcg ccaaactggt gaatcaggga
    53101 gagtccgttg ccatctgcga acaaattggc gatccggcga ccagcaaagg tccggttgag
    53161 cgcaaagttg tgcgtatcgt tacgccaggc accatcagcg atgaagccct gttacaggag
    53221 cgtcaggaca acctgctggc ggctatctgg caggacagca aaggtttcgg ctacgcgacg
    53281 ctggatatca gctccgggcg ttttcgcctg agcgaaccgg ccgaccgcga aacgatggcg
    53341 gcagagctgc aacgcactaa tccggcggaa ctgctgtatg cagaagattt cgccgagatg
    53401 tcgctgattg aaggccgtcg cggcctgcgc cgtcgcccgc tgtgggagtt tgaaatcgac
    53461 accgcgcgcc agcagttgaa tctgcaattt ggcacccgcg atctggtcgg ttttggcgtc
    53521 gagaacgcgc cgcgcggact ttgtgctgcc ggttgtctgt tgcagtatgc gaaagatacc
    53581 caacgcacga ccctgccgca tattcgttct atcaccatgg aacgtgagca ggacagcatc
    53641 attatggatg ccgcgacacg tcgtaacctg gaaattactc agaacctggc gggcggtgcg
    53701 gaaaatacgc tggcttctgt gctcgactgc accgtcacgc cgatgggtag tcgtatgctg
    53761 aaacgctggc tgcatatgcc agtgcgcgat acccgcgtgt tgcttgagcg ccagcaaact
    53821 attggcgcat tgcaggattt caccgccggg ctacagccgg tactgcgtca ggtcggcgac
    53881 cttgaacgta ttctggcacg tctggcttta cgaactgctc gcccacgcga tctggcccgt
    53941 atgcgccacg ctttccagca actgccggag ctgcgtgcac agttagaaac tgtcgatagt
    54001 gcaccggtac aggcgctacg tgagaagatg ggcgagtttg ccgagctgcg cgatctgctg
    54061 gagcgagcaa tcatcgacac accgccggtg ctggtacgcg acggtggtgt tatcgcatcg
    54121 ggctataacg aagagctgga tgagtggcgc gcgctggctg acggcgcgac cgattatctg
    54181 gagcgtctgg aagtccgcga gcgtgaacgt accggcctgg acacgctgaa agttggcttt
    54241 aatgcggtgc acggctacta cattcaaatc agccgtgggc aaagccatct ggcacccatc
    54301 aactacatgc gtcgccagac gctgaaaaac gccgagcgct acatcattcc agagctaaaa
    54361 gagtacgaag ataaagttct cacctcaaaa ggcaaagcac tggcactgga aaaacagctt
    54421 tatgaagagc tgttcgacct gctgttgccg catctggaag cgttgcaaca gagcgcgagc
    54481 gcgctggcgg aactcgacgt gctggttaac ctggcggaac gggcctatac cctgaactac
    54541 acctgcccga ccttcattga taaaccgggc attcgcatta ccgaaggtcg ccatccggta
    54601 gttgaacaag tactgaatga gccatttatc gctaacccgc tgaatctgtc gccgcagcgc
    54661 cgtatgttga ttattaccgg cccgaacatg ggcggtaaaa gtacctatat gcgccagacc
    54721 gcactgattg cgctgatggc ctacattggc agctatgtac cggcacaaaa agtcgagatt
    54781 ggacctatcg atcgcatctt tacccgcgta ggcgcggcag atgacctggc ttccgggcgc
    54841 tcaaccttta tggtggagat gactgaaacc gccaatattt tacataacgc caccgaatac
    54901 agtctggtgt taatggatga gatcgggcgt ggaacgtcca cctacgatgg tctgtcgctg
    54961 gcgtgggcgt gcgcagaaaa tctggcgaat aagattaagg cattgacgct gtttgccacc
    55021 cactatttcg agctgaccca gctaccggag aaaatggaag acgtcgccaa cgtgcatctc
    55081 gatgcgctgg agcacggaga caccattgcc tttatgcata gcgtgcagga tggcgcggcg
    55141 agcaaaagct acggcctggc ggttgcagct ctggcaggcg tgccaaaaga ggtgattaag
    55201 cgcgcacggc aaaaactgcg tgagctggaa agcatttcgc cgaacgccgc cgctacgcaa
    55261 gtggatggta cgcaaatgtc tttgctgtca gtaccggaag aaacctcgcc agcggtcgaa
    55321 gctctggaaa acctggaccc ggattcactg actccgcgtc aggcgctgga atggatttat
    55381 cgcttgaaga gcctggtgta ataacaattc ccgatagtct tttgctatcg ggaatattaa
    55441 cgacaactga cgaataaaat aaaaaccccc tgtataatag gaaagcttat tttacagggt
    55501 aaaaccatgc catctacacg ctatcaaaaa attaatgccc atcactatcg ccatatatgg
    55561 gtcgttggtg atattcatgg tgaatatcaa ttattacaat cccgcttaca tcaactctct
    55621 ttttgccctg aaaccgactt acttatttct gtcggcgata atattgatcg tgggccggag
    55681 agtcttgacg tcctgcgcct gctaaaccag ccctggttta tctccgttaa aggcaaccac
    55741 gaagcaatgg agctggatgc gttcgcgacc ggcgatggca atatgtggct tgccagcggt
    55801 ggtgactggt ttttcgattt aaatgattca gagcaacaag aggcaataga tctgttgctg
    55861 aaattccatc accttccaca tattattgaa atcactaacg acataataaa atatgtcatc
    55921 gcacatgcag attatccggg tgatgaatat ctctttggca aagaaatagc ggagagcgaa
    55981 ttactctggc ctgtttatcg tgtgcagaaa tcgcttaatg gcgagttaca acaaataaac
    56041 ggcgctgatt attttatatt tggacatatg atgtttgata acattcagac gttcgctaac
    56101 cagatttata ttgataccgg atcgccgaaa agtgggcggc tgtcatttta taaaataagg
    56161 tagtctcatg cttcttctgt gaagcatgag taaccagatg ttattgcagg ccattattca
    56221 tttttcgcta ccagcaaaga gagatcctgc ttcgccagcg cgcgactggc actctccggc
    56281 aaaccgtcgt ctgtaataat ctgatcaaac tcgcttaatg gtaacgccag ccatgtcgcc
    56341 acctgaccat atttcgtcgc atcacagacc aaaactcgct ggcggctggc actagcaatc
    56401 gcccgtttca ccgtgacttt atcttccgct ggcgtagaaa tcccccgcac actccatgac
    56461 gatgcagaaa taaaagcctg atcaatcatc aggctgcgca gcatggtcgc agcggcttcc
    56521 ccgacacagg aacggttttc ccgacacact gcaccgccag tgtgaataat tgtgcaatta
    56581 ctgttgtcga gcaagtagtt cgcaataacg aaatcgtttg tgaccacagt cagtgactcc
    56641 atgtgaatca gatgctgtgc tatcgctaac gtggtcgttc ccgcatccag atagatacaa
    56701 cttcccggct gaacaagact tgccgccagc ttgccaatag ccgctttttg cgtcattgct
    56761 agcgcagttt ttacctgatg agaaggttca tgcgccacgc gtcccggaga ctggacgcct
    56821 ccggacacca gcacaacggc tccctgctgc tccagttttt gtaaatcccg acgaatggtc
    56881 atatgtgata cattcattct gtccgttagt tcagcaatac tgacaatgcc tttttcagct
    56941 accatctcaa ggatgatttg gcgacgctct acgggtatca acttttgctc cttcctttgt
    57001 cctgctgaca ttctacgcta tttgcctgcg aaacgtgcgc ggcgcaacta acgcttagtt
    57061 cacataaaat aacacacaat gttaatttat gtgaataggc tcaccatatc gttatcttcc
    57121 agcgcttata ttcacaatat caaacaaaat atcacttaaa ttaacaagga gagcagatga
    57181 aaacgggatc tgagtttcat gtcggtatcg ttggcttagg gtcaatggga atgggagcag
    57241 cactgtcatg tgtccgcgta ggtctttcta cctggggcgc agacctgaac agcaatgcct
    57301 gcgctacgtt gaaagaggca ggtgcttgcg gggtttctga taacgccgcg acgtttgccg
    57361 aaaaactgga cgctctgctg gtgctggtgg tcaatgcggc ccaggttaaa caggtgctgt
    57421 ttggtgaaac aggcgttgca caacatctga aacccggtac ggcagtaatg gtttcttcca
    57481 ctatcgctag tgctgatgcg caagaaattg ctaccgctct ggctggattc gatctggaaa
    57541 tgctggatgc gccagtttct ggtgatgcag taaaagccgc taacggtgaa atgactgtca
    57601 tggcctccgg tagcgatatt gcctttgaac gactggcacc cgtgctggaa gccgttgccg
    57661 gaaaagttta tcgcataggt gcagaaccgg gactaggttc gaccgtaaaa attattcacc
    57721 agttgttagc gggcgtacat attgctgccg gagccgaagc gatggcactt gcagcccgtg
    57781 cggggatccc gctggatgtg atgtatgacg tcgtgaccaa tgccgccgga aattcctgga
    57841 tgttcgaaaa ccggatgcgt catgtggtgg atggcgatta caccccgcat tcagccgtcg
    57901 atatttttgt taaggatctt ggtctggttg ccgatacagc caaagccctg cacttcccgc
    57961 tgccattggc ctcaacagca ttgaatatgt tcaccagcgc cagtaacgca ggttacggga
    58021 aagaagacga tagcgcagtt atcaagattt tctctggcat cactctaccg ggagcgaaat
    58081 catgatcaag attggcgtta tcgccgatga ttttaccggc gcgacggata tcgccagttt
    58141 tctggtggaa aacggtctac caacggtaca aattaacggt gttccaacag gtaaaatgcc
    58201 ggaagcaatc gacgcactgg tgatcagcct gaaaacgcgc tcctgtccgg tggttgaagc
    58261 cacacagcaa tcgctggcgg ctctgagctg gttgcaacag caaggttgca aacagatcta
    58321 tttcaaatac tgctctactt tcgacagtac ggcgaaaggt aatatcggcc cggttaccga
    58381 tgcattaatg gatgctctcg acacgccgtt tacggtcttc tctccggccc tgtcggtcaa
    58441 cggacgtacg gtttatcagg ggtatttgtt cgtaatgaat caactgctgg ccgaatccgg
    58501 gatgcgccat cacccggtaa atcccatgac cgacagctat cttccccgtc tggttgaagc
    58561 gcaatccaca gggcgctgcg gcgtcgtttc ggcacatgtt ttcgaacaag gtgtggatgc
    58621 cgttcgtcaa gagctggctc gcttacagca agagggctac cgctacgcgg tgcttgatgc
    58681 gctgaccgaa caccatctgg aaattcaggg agaagccttg cgcgatgccc cactggtaac
    58741 gggcggttct ggtctggcga ttggcctggc ccggcagtgg gcgcaagaaa acggtaacca
    58801 ggctcgcgaa gcagggcatc cgctcgctgg gcgcggcgta gtgctctccg gttcatgctc
    58861 tcaaatgacc aaccgccagg tggcacatta ccgtcaaatt gcaccagccc gtgaagttga
    58921 tgtggcacgc tgcctctcaa ccgaaactct ggccgcttat gcacacgaac tggcagagtg
    58981 ggttctgggc caggaaagtg tacttgctcc actggttttt gccaccgcca gcactgacgc
    59041 attggcagca attcaacagc aatacggtgc acaaaaagcc agtcaggcag tagaaactct
    59101 gttttctcaa ctagcggcgc ggttagcagc ggaaggcgtg acacgcttta ttgtcgcagg
    59161 cggtgagacc tccggcgtag tcacacagag cctgggcata aaagggtttc atattggccc
    59221 aaccatttcc cccggcgtgc cgtgggtaaa cgcactggat aagcctgtct cactcgccct
    59281 taaatctggc aacttcggtg atgaagcctt tttttcacga gcccaaagag agtttttatc
    59341 atgagcgatt tcgcaaaagt agagcagtct ttgcgagagg agatgacgcg gattgccagt
    59401 tcattctttc agcgcggcta tgcaaccggt tcggctggca atctgtcgct gcttttacct
    59461 gacgggaatt tactggcgac accgacaggt tcatgcctgg gcaatctcga tccgcagcgg
    59521 ctttccaaag tcgccgcgga tggcgaatgg ttaagtggtg acaaaccctc gaaagaggtg
    59581 ctctttcatc tggcgctgta tcgcaacaat ccgcgctgta aagcggtggt gcatttgcac
    59641 agcacatggt cgacggtgct ttcctgcctg caagggctgg acagcagcaa cgttattcgt
    59701 ccgttcacac catacgtggt gatgcggatg ggaaatgtcc cgctggtgcc ctattaccga
    59761 ccgggcgata aacgcatcgc acaggatctg gcggaactgg cagcagacaa tcaggctttt
    59821 ttactggcaa atcatggccc agtggtttgc ggtgaaagcc tgcaagaagc cgccaacaat
    59881 atggaagagc tggaggaaac ggcaaagctg atttttattc tcggtgaccg cccgatccgt
    59941 tatctgaccg caggtgaaat tgcggaatta aggagttaat gcaatgcctc gttttgcagc
    60001 taatttatcc atgatgttca ccgaagtgcc ttttattgaa cgcttcgccg cagcgcgaaa
    60061 agccgggttc gatgctgtgg aatttctgtt tccctatgac tactccaccc tgcaaatcca
    60121 aaagcaactg gagcaaaacc atctgacact gcgctgttca ataccgctcc cggagatatt
    60181 aatgcggggg aatggggatt atccgccctt cccggacgtg agcacgaagc acacgcagac
    60241 atcgacctgg cactggaata tgcattggcg ctcaattgtg aacaagtcca tgtgatggca
    60301 ggcgtcgtgc ccgctggcga agatgctgaa cggtaccggg cagtatttat cgataatctc
    60361 cgctacgctg ccgaccgctt tgcgccacac ggcaagcgaa tattagttga agcactcagc
    60421 cccgacgtta agcctcatta tctcttctcc agccagtatc aggcactggc tattgttgaa
    60481 gaggttgcgc gagataacgt gtttattcaa ctagatactt ttcatgcgca aaaagtagat
    60541 ggcaacctga cgcatttaat ccgcgactac gctgggaaat atgcgcacgt acaaattgcc
    60601 ggactacctg atcgacatga ccggacgatg gagaaatcaa ctatccgtgg ctgttccgcc
    60661 tgttcgatga ggtgggatat cagggctgga tcggttgtga atataaacct cgtggcctca
    60721 ccgaagaagg gcttggctgg tttgacgcct ggcgctaatt cgttaaatcc ctaattacaa
    60781 cgtacccata catcccccca tctacggatg gggtaggtta actataactt tcagacaggg
    60841 tttcccatgt ccacaattac attgttatgc attgcgttag ctggcgtaat catgctgttg
    60901 ctgctggtca tcaaggcaaa ggtacaacca ttcgttgctc tgctcctcgt cagcctgtta
    60961 gtcgcacttg cggcgggtat accggcgggc gaagtgggta aagtgatgat cgccgggatg
    61021 ggcggtgtgc ttggttccgt cactattatt attggtctgg gcgctatgct ggggcgtatg
    61081 atcgaacact ctggtggtgc agagtcactg gctaattatt tcagtcgcaa gttaggtgac
    61141 aaacgaacta tcgctgcgct gactctggca gcgttcttcc tcggtattcc cgtcttcttt
    61201 gatgtcagct ttattattct tgcgccaatc atttacggtt ttgccaaggt tgccaaaata
    61261 tcgccactca aatttggcct gcctgtcgct ggaatcatgc taactgttca tgtggcagta
    61321 ccgccgcatc caggccctgt cgccacagcg gggttactcc acgcagacat cggctggcta
    61381 accatcatcg gtattgcgat ttctattccc gtagggattg ttggctactt tgcagcgaaa
    61441 ataatcaata agcgccaata tgcgatgtca gtagaagtac tggaacagat gcaactggct
    61501 ccggccagtg aggaaggcgc gacaaaatta agcgataaaa taaatccacc gggcgtcgcg
    61561 ctggtcacct cgctaattgt tattcctatc gcgattatca tgacgggtac ggtttccgca
    61621 acactgatgc cgccttcgca tcccctgctt ggtacgctac agctgatcgg ctcaccaatg
    61681 gtagctctaa tgattgcgct ggtgctggca ttctggttat tggctttacg tcgcggctgg
    61741 agcttacaac acaccagcga cattatgggc tcagcccttc ccactgcggc ggtagtgatt
    61801 ttggttactg gtgctggagg ggtatttggc aaagtgctgg tggaatcggg cgtcggcaaa
    61861 gcccttgcca atatgctaca aatgattgac ctgcctctgt taccagccgc atttattatt
    61921 tcactggctc tgcgtgcatc gcaggggtca gcaaccgtag caatcctgac aaccggcggg
    61981 ttactctcag aagcggtgat gggattgaat ccgattcagt gcgtattggt gacgctggca
    62041 gcctgctttg gtgggcttgg cgcgtcacat attaatgact cagggttctg gattgtgacc
    62101 aaatatctgg ggttgtcggt agcagacggt ctgaaaacct ggacagtgtt aacgaccatt
    62161 ctcggtttta ccggattctt aattacctgg tgcgtatggg cggtaatttg accttaataa
    62221 aaaggtccga tgggcatcgg accttttatt gtgcacagaa aaggccagcc tcgcttgaga
    62281 ctggcctttc tgacagatgc ttacttactc gcggaacagc gcttcgatat tcagcccctg
    62341 cgtttgcagg atttcacgca aacggcgcag gccttcaacc tgaatctggc gaacacgttc
    62401 acgggtgagg ccaatttcac gacctacatc ttccagtgtt gccgcttcgt accccagcaa
    62461 accgaatcga cgtgccagca cttcacgctg tttggcgttc agctcgaaca gccatttgac
    62521 gatgctctgc ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct ctttttcatc
    62581 ggccaggatg tccagcaacg ctttttcgga atcaccaccc agcggggtgt ctaccgaggt
    62641 aatgcgctcg ttaagacgaa gcatacggct gacgtcatca actggcttat ccagttgctc
    62701 tgcgatctct tccgcacttg gttcatggtc cagcttatgg gacaactcac gtgcggttcg
    62761 caggtaaacg ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac gggtttggtt
    62821 cataatcgcc cgttcaatcg tctggcgaat ccaccaggtt gcgtatgttg agaagcggaa
    62881 accacgttcc gggtcaaact tctctaccgc gcggatcagc cccaggttgc cctcttcgat
    62941 aaggtccagc aacgccagac cacgattgcc ataacggcgg gcaattttta ccaccagacg
    63001 caagttactc tcgatcatcc ggcggcgaga ggcgacatct ccacgcagtg cgcgacgcgc
    63061 aaaataaact tcttcttcgg ccgttaacag tggtgaataa ccaatctcac caaggtaaag
    63121 ctgagtcgcg tccaacactc gctgtgtggc tccctgcgat aacagttcct cttcggccaa
    63181 atcgttatca ctgggttcct cttctactaa ggccttttcg tcaaaaacct caactccgtt
    63241 ctcatcaaat tccgcatctt catttaaatc atgaactttc agcgtattct gactcataag
    63301 gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcctggt tccgccgatt
    63361 tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg aatttcaaaa
    63421 tgcaagcgtg ttgaactggt tccggtgcta cccatggttg ctattttttg ccccgccttc
    63481 acttcttgtt gttcccggac cagcattgtg tcgttatggg cgtaggcact caggtaatca
    63541 tcattatgtt tgatgataat cagattaccg tagccgcgca gcgcgttacc ggcataaaca
    63601 acgcggccat ctgcggtcgc gataattgcc tgtcctttgc tgcctgcgat atcaatcccc
    63661 ttgttgcccc cctcagaagc gccaaaggtt tcgatcactt tgccctcagt cggccagcgc
    63721 caggtggaga taggcgtact ggttgatgta ctgctgacag ttggctcggt tgtgcttgct
    63781 gttggtaccg ttacaggcgc tgtgaccgtg gtcgcagctg gcttgttgtt cggcaacatt
    63841 ttgttagcac tctgttcacc cgaagattca gaatacgtaa ttgtcggttg cgacgcaaca
    63901 gcaacggtgg aattttgtgc aggcttgatc acaactcctt gctctgctgc gtcggcctgg
    63961 gtaatggcat ttccgccagt gattggcgta ccggaagcat tacccacctg caaggtctga
    64021 ccaacgttca gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc acggaaatcg
    64081 ttgccagtaa tccaggcgat atagaaaagt gtgtcgcctt ttttcacggt ataggtactg
    64141 ccgctataac tgcctttcgg aatgttccca tactgacggt tatagacgat gcgtccgttt
    64201 tccatctgta ccggctgctg agctactggc tgcactggct ggatttgcgg ttgctgggct
    64261 gtagacgtcg tccccatttt cggcggcggc gtaatcaaca taccagaatt ggtatttgca
    64321 ggcgcattgc cattaacgga gctgaccggt gccggtggat ttgaagtgtc agaacagcct
    64381 gccagccata gcgaaaccag tgacaaagcc gcaatgcggc gaacggtgaa ttttgggctt
    64441 cccgcgctca tttatccccc aggaaaaatt ggttaataac cagtgacata attaccgtgc
    64501 aaggcaccct actgaacact ggaaaagatg ttcacgatac gctgacctgc ggcaaaataa
    64561 ccaggaaaaa tccaggtatt tcctcacgtt ttaagccagc tcccccttca ctaaagggac
    64621 aaagcgcacg gcctccacgg tatcgataat aaattcgcct ccccgacgac gcacccgttt
    64681 caaatactgg tgctcctccc ctacgggtaa gacgagaatc ccgccttcgt ccagctgcgt
    64741 cattagcgca gttggaattt ccggcggtgc cgccgtaaca atgatagcgt caaacggcgc
    64801 gcgtgcctgc caaccttgcc atccatcgcc atgacgggtt gaaacattat gtaaatcaag
    64861 atttttcagg cggcgacgcg cctgccactg caaaccttta atccgttcga ccgagcaaac
    64921 atgctggaca agatgcgcca ggattgccgt ttgatatccc gaaccggtgc caatttccag
    64981 cacccgcgac tgcggcgtca gctcgagtaa ttcggtcatt cgcgccacca tatacggctg
    65041 cgaaattgtc tgcccctgac ctatcggcaa ggcgatattg tcccaggctt tttgttcaaa
    65101 cgcttcatca acgaattttt cacgcggcac ggcagcaagt gcattcagca cctgctcatc
    65161 ctgaatacct tgcgcacgta attgatccag aagtgcttgt acgcgtctgc ttaccattgc
    65221 gtgccaactc ccacgctgtt taaccagtct gaaaccacat cttgcgcgct atgcgcagtt
    65281 aaatccacat gcagcggcgt gatggagaca tagccctcat ctaccgcagc aaaatcggtc
    65341 cccggaccgg catcacattt accgcccggc gggccaatcc agtacagcgt attgccgcgc
    65401 ggatcttgct gcgggatcac ctgatctgcc ggatgtcgtg taccgcagcg cgtcacgcga
    65461 atacctttga tttgatccaa gggtaaatcc ggaacgttaa tattaagaat acgtccggtg
    65521 cgcagcggct ctttacacag tgcgcgcaaa attgaacagg ttaccgccgc ggcagtgtcg
    65581 taatgtttat gcccgtcaag cgagacggcc agcgccggaa aacctaaatg acggccttcc
    65641 atcgcggcgg ctaccgtacc ggaataaata acgtcatccc ccagattcgg cccggcgtta
    65701 atgccggaca caacaatgtc cgggcgcgga cgcatcagag cattcacgcc aagatagacg
    65761 caatcggtcg gggttcccat ttgcacagca atatcaccat tttcaaaggt aaacgtgcgc
    65821 agggaggatt ccagtgtcag agaatttgaa gcgccgctgc ggttacgatc gggggcgacc
    65881 acctgaacgt cagcaaactc acgcaaggct ttcgccagcg tttgtatacc gggtgcatgt
    65941 accccgtcat cattactcag caatatgcgc ataatcacct gttgtgttga taagttccct
    66001 gacaacgctg gttgcaaaac tacccgccgg aagccagaaa cggatctcta cggtgacgtc
    66061 atcccaccaa ttccagctta attgttgcgg atacagcagc atcgctctgc gcgcggcttc
    66121 aactttttcg cgcaccagta aagcttgtaa ttcagtttct gcggcgacag ctgcttgttc
    66181 gaatgccagc gctttacgct gagttcccca ttcgccactg cccggcaatg cggcggttat
    66241 catcagctct ttatcgttga cgcgatgctg taattccgcc agttcttcgg tggttgctac
    66301 aaaccagcta ccacgtccgg ctaattgtag cgcatcgccg tcaacaactt gattaacgtc
    66361 tgcttttttg aggcgctcag caacaatctg attaaacaac gcactgcggg ctgccgacaa
    66421 ccaaaaactc cgtttattgc gatcgcgcac cggagtattg gtttgcgccc agcgcagcgc
    66481 gccctgcaag ttgctaccgc caatcccaaa acgttgggca gcgaagtagt tcggtacacc
    66541 ttttacgcaa atatcgatca gacgttgttc aacgtcatcg cgattgctca cttcgcgtag
    66601 aaccagggta aaggcgttac ctttcagcgc gcctaaacgc agcttgcgct tgtgccgcgc
    66661 atactccagc acctggcagc cttccagttg aaaggcgctc agatcgggca tttccttgcc
    66721 tggcacgcga gcgcataacc actgttccgt aacagcatgt ttgtcttttt gcccagcgaa
    66781 gctgacttca cgggcatgaa ttttcaggaa tttcgccagt gcatccgcca caaaacgggt
    66841 attgcagccg tttttgagga ttctaaccag aatatgctca ccttcaccat caggctcaaa
    66901 gcccaaatct tccaccacca caaagtcttc cggattggct ttcagcagcc cggtgccttg
    66961 cggtttaccg tggaggtaag tgagattatc aaactcaatc attttgttgc cttaatgagt
    67021 agcgccaccg cttcacaggc aatcccttcc ccacgtccgg taaatccaag tttttccgta
    67081 gtagtggctt tcacgttaac atcatccata tggcagccga gatcttcggc aataaacacg
    67141 cgcatttgtg gaatgtgcgg caacatcttc ggtgcctgag cgatgatagt gacatcgacg
    67201 ttgcccaggg tataaccctt cgcctgaata cgacgccagg cttcgcgtag cagctcgcgg
    67261 ctgtcggcac ctttaaatgc cggatcggta tccgggaaca gcttgccgat atcccccagc
    67321 gccgccgcgc caagcaatgc atcggtcaac gcatggagcg ccacgtcgcc atcagaatgc
    67381 gccagcaatc ctttttcgta aggaatgcgt acgccaccaa tgataattgg gccttcaccg
    67441 ccaaaggcat gtacgtcaaa accgtgtcca attcgcatta tgtattctcc tgatggatgg
    67501 ttcgggtgag gtaaaactcg gccagtgcca aatcttccgg gcgcgtgact ttaatgttat
    67561 ccgcacggcc ttcgaccaac tgaggatgga atccgcaata ttccagcgcc gaggcttcgt
    67621 cggtaatagt cgcgccttca tttagagcgc gcgtcagaca gtcatgtaac agctcacgag
    67681 ggaaaaattg cggcgtcagc gcgtgccata agccgttgcg atcaacggta tgagcaatgg
    67741 catttttgcc cggttcggca cgtttcatcg tatcgcgcac tggtgcggct aggatccctc
    67801 ccgtgcggct ggtttcgctc aacgccaaca atcgcgcgag gtcatcctga tgcagacaag
    67861 gacgagcggc gtcatgcacc aatacccact gcgcgtcgcc agcggctttc aaacctgcca
    67921 gcacggaatc ggcacgctca tcaccgccat ctacaacggt gatttgcgga tgattcgcca
    67981 gaggaagttg tgcaaaacgg ctatcgccag gacttatggc aatgacgaca cgtttcaccc
    68041 ggggatgcgc cagcagcgca tgcaccgagt gttcaagaat ggtttgatta ccgattgaga
    68101 gatattgctt aggacattcc gtttgcattc gacggccaaa tccggccgcc ggaaccacgg
    68161 cgcaaacatc caaatgagtg gttgccatgt taattcccgg gctgatttat cgattgtttt
    68221 gccccgcaga ctgtgcgcgc ttcgacgcgt caggcaccag acgataaaaa gtttcgcccg
    68281 gcctggtcat gctgagttca ttacgcgcac gctcttcgag cgcctcctgg ccgccattga
    68341 gatcgtcaat ttcggcaaaa agttgatcgt ttcgcgcttt aagtttcgcg tttgtagctt
    68401 gctgtgccgc cacatcatca ttgacgcggg tatagtcatg tataccgttc ttaccgaacc
    68461 acagcgaata ctgtagccag accagaatag ccagcaacag cagcgttagt ttacccatcc
    68521 tgccccctga aaaacggcat catcatccca tgcatccgaa gacgactcta catcctctgt
    68581 tggggatacc gcgacaacgc gggcaaatgt accacatttg tccattgtta cgtataccta
    68641 gggcgtgcag aacataatct cattattagt tacggtttga attatgaaca gaggagacaa
    68701 gaaagtacaa attagcccag tagccacata aacagtgcgc caaacataat gcctactgtc
    68761 accagggtga aaacaatact gtagcgtagc tttccgtcca tcaatgaatg cagcgcaatc
    68821 cccaccacta ccgcgacagg catcagtgcc agaaagaaag gccaggtgta gataaagaag
    68881 aacagcgtgt tagagccata aatcaacatc ggcatcgcca gcgcaaataa ccaggagata
    68941 aaaccgacca cggcaccagg cagtgaccat gtggtttctt catcctcagt aaggctgtcg
    69001 ttatttgtta gtgtaatgtt atggctatta cgcatatttg atcctgttac tttgacgaac
    69061 cgggcatgga aacccggtgg tgtctcagga tctgataata tcgttctgtc tcaacagatc
    69121 taataattgc tgtaccaaat ttgttactaa ttgttcacca ttgagatgaa tttctgccga
    69181 ttcaggcgct tcgtaaacgg aatctattcc cgtaaagttg cgcagttcac cggcacgcgc
    69241 tttcttatat aagcctttgg gatcgcgggc ttcgcaaatc gccagcggcg tatcgacaaa
    69301 cacttcgata aagcgccctt ctcctacgcg ttcgcgaacc atctggcgtt cggcgcggtg
    69361 tggcgagata aatgcggtca gcaccaccag tccggcttca accatcaaat tcgccacttc
    69421 accgacgcga cggatattct ctttacgatc ggcatcgcta aaaccgagat cgctgcataa
    69481 tccgtggcga acattgtcgc catccagcag atacgtactg acgccgagtt tatgtaacgc
    69541 ctcctccagc gccccggcga ccgttgattt accggaccca gagaggccgg taaaccacag
    69601 cactacacca cgatgaccgt ggtgtagctc gcgttgttgc acagtgaccg gatggctatg
    69661 ccagacgacg ttttcgtcat gcagcgccat tatttatccc ccagcaaatc gcgcgcgccc
    69721 cagtgcggaa agtggcgacg aaccagggca ttcaattcca gttcgaatgc actgaattca
    69781 gatgacgcaa cggcggcctc gtttttcggc tcatgcacca taccggcacc aacagtcacg
    69841 ttgctcaggc gatcgataaa aatcagccca cccgtcaccg gattttgttg ataacgatct
    69901 aacaccagcg gctcgtcaaa agtgagatcc acgaggccga tcccattcag tggcaggttt
    69961 tcaacttcac gctgggtaag gttattaata tcaaccggat agcgaatgcc atcaacacgc
    70021 gcgcgcgtct tcttaccggc aattttgatg tcgtaactct ggcccgggga aagcggctgt
    70081 tccgccatcc ataccacatc caccgacgcg ctcggcaccg ccggtaacgc ttcgtctgcc
    70141 gccagcagca gatcgccacg gctgatgtcg atctcatccg tcagcaccag ggtgatcgct
    70201 tctccggcaa aggcttcttc gcgatcacca tcaaaagtca cgatccgcgc gacgtttgat
    70261 tccacaccag agggcagcgc tttgacacgt tgcccgactt ccacgcgacc ggatgccagc
    70321 gttccggcgt agccacgaaa atcgagattc gggcgattaa cgtactgcac cgggaagcgc
    70381 attggctggg catccaccac tcgctggatc tccacggttt ccagcacttc gagcagtgtc
    70441 ggaccgctgt accacgacat actttcactt tgcgatgcca cgttgtcgcc ttccagtgca
    70501 gagagcggca caaagcggat atccagatta cccggcagct gcccggcaaa ggtcaaataa
    70561 tcttcacgaa tatgggtgaa cgtctcttca ctgtaatcca ccagatccat tttgttgatc
    70621 gccacgacca gatgtttgat ccccaacagt gtggagataa aactgtgacg acgggtttga
    70681 tcgagcacgc ctttacgggc atcgatcagt aagatcgcca gttcacatgt cgatgcgcca
    70741 gttgccatat tgcgagtgta ctgctcgtgc cctggggtgt cggcgataat aaatttacgc
    70801 ttctcggtag agaaataacg gtaagccacg tcaatggtga tgccctgttc gcgctcagct
    70861 tgcaggccgt ccaccagcag tgccagatcc agcttttcgc cctgggtgcc gtgacgctta
    70921 ctgtcgttat gcagcgatga gagctgatct tcgtagattt ggcgggtatc gtgcagcagg
    70981 cgaccaatca gggtactttt gccgtcatcg acgctaccac aggtcagaaa acgcagcagg
    71041 cttttatgtt gttgcgcaat catccaggct tcgacgccgc cttcattggc gatttgttgt
    71101 gcaagtgcgg tgttcatctt aaaaataccc ctgacgtttt ttcagctcca tagaccccgc
    71161 ctggtcgcgg tcaatcacgc ggccctgacg ttcactggtg gtagaaacca gcatctcttc
    71221 gatgatctcc ggcagtgttt gtgcatttga ctccaccgca ccggtcagcg gccagcagcc
    71281 cagcgtacgg aaacgcacca tccgtttttt aatcacttcg cccggttgca ggtcgatacg
    71341 gttgtcatca atcatcatca acataccgtc gcgttccaga accggacgtt ccgcagcgag
    71401 atacagtggc acaatgtcga tattttccag ccagatgtat tgccagatat cctgctcggt
    71461 ccagttagag agcgggaaga cgcggatgct ttcacctttg ttaatttgcc cgttgtagtt
    71521 gtgccacagc tccgggcgct gatttttcgg atcccagcga tggaagcggt cacggaaaga
    71581 gtagattcgc tctttagcgc gggatttctc ttcatcacgg cgcgcaccac cgaaggcggc
    71641 atcaaaaccg tatttgttca gcgcctgttt caggccttca gttttcataa tatcggtatg
    71701 tttcgcgctg ccgtgtacga atggattaat ccccatcgcc acgccttccg ggtttttatg
    71761 caccagcagc tcgcagccgt aggctttagc agtacgatcg cggaactcat acatctcgcg
    71821 gaatttccag ccggtatcga catgcagcaa cgggaaaggc agcgtacctg gataaaacgc
    71881 cttgcgcgcc agatgcagca tgacgctgga atctttaccg atagagtaga gcatcaccgg
    71941 atttgagaat tctgccgcca cctcgcgaat aatgtggatg ctttccgcct ccagttgccg
    72001 caggtgagta agtcgttttt gagccataac cgttcctttg caataccgct attttcttgc
    72061 catcagatgt ttcgactata gggagcgtaa gagaacgaat gaaattacca attagaatga
    72121 gtagttcctt aacggaataa cgatttggca aagctaatat caaaaagtgc ttaaggcacc
    72181 ggatttcggg cgtttaggaa gatttgaaat tgttttagcg cagcggcagt ttcatactat
    72241 ggcggtaaaa aaatttgcat ggtatttaag gactcactat gttttccgca ttgcgccacc
    72301 gtaccgctgc cctggcgctc ggcgtatgct ttattctccc cgtacacgcc tcgtcaccta
    72361 aacctggcga ttttgctaat actcaggcac gacatattgc tactttcttt ccgggacgca
    72421 tgaccggaac tcctgcagaa atgttatctg ccgattatat tagccaacag tttcagcaaa
    72481 tgggttatcg cagtgatatt cggacattta atagtcggta tatttatacc gcccgcgata
    72541 atcgtaagag ctggcataac gtgacgggaa gtacggtgat tgccgctcat gaaggaaaag
    72601 cgccgcagca gatcatcatt atggcgcatc tggatactta cgccccgctg agcgatgctg
    72661 acgccgatgc caatctcggc gggctgacgt tacaaggaat ggatgataac gccgcaggtt
    72721 taggtgtcat gctggaattg gcagaacgcc tgaaaaatac gcctaccgag tatggtattc
    72781 gatttgtggc gaccagcggc gaagaggaag ggaaattagg cgctgagaat ttactcaagc
    72841 ggatgagtga caccgaaaag aaaaatacgc tgctggtgat taatctcgat aacttaattg
    72901 ttggcgataa attgtatttc aacagcggtg taaaaaccac tgaagcagta aggaaattaa
    72961 cgcgcgacag ggcgctggca attgcgcgca gtcacggaat agccgcaacg accaatccgg
    73021 ggttgaataa aaattatccg aaaggcactg ggtgttgtaa tgacgcagaa atattcgaca
    73081 aagcgggcat tgctgtactt tcggtggaag cgactaactg gaatcttggg aataaggatg
    73141 gttatcagca acgcgcaaaa acagccgcat tccctgcggg aaatagctgg catgacgtaa
    73201 gactggataa tcaacagcat attgataagg cacttcctgg aagaatagaa cgtcgctgcc
    73261 gtgacgttat gcggataatg ctaccgttgg tgaaggagct ggcgaaggcg tcttgatggg
    73321 tttgaaaatg ggagctggga gttctaccgc aggggcgggg aattctaagt gatatccatt
    73381 atcgcatcca gtgcgcccgg tttatccccg ctggcgcggg gaacacacta agcatacata
    73441 actgttttta aacaaattta ttccgcatca acaatctacc aactaaatta aaacatttcc
    73501 ttatttttaa agaacggata acttattgat tatcaacagg aagaaaagaa accaaacgta
    73561 acccatccaa atccaccgga atacgtctgt tttctcccca ggtctgaaat tcaaaacccg
    73621 actcggtatt ggtcgcccag gccatcacca catttccgca accagccagt tgggtaattt
    73681 gctgccagat catctcccga atacgttttg atgtatcacc aacatacaca ccggcacgca
    73741 cttccagtag ccagattgcg agccgtccac gtaagcgtgg cgggacattt tctgtaacaa
    73801 ccacgaccat gctcatcagc cgcgtcctcg atgaccgcta tcgcccaacg tttcaggttc
    73861 ggggatggct ggcggtaaca tatccggcgc aggttgtggc ggttcaattt cacctgcggc
    73921 aaggacttcc tcaattaacg gtattaattt gcccgttaac ttagtgctac ggaaaatatc
    73981 acgacaggct aatctgactt ctttatcagg ttctgcgggt tgcctcgccg ctatttcaaa
    74041 tgcctttggt acaaccgaat caaatttaat gatatcggct atgtcataaa caaatgaaag
    74101 cggtttgcca ctatggataa acccaatagc gggcgcatat cccgcggcta atactgccgc
    74161 ttcagatata ccgtacagac atgatgtagc agcactgatg cagcgattca caacatcgcc
    74221 tttttcccag tctttaggat cgtatttgcg accattccat ttcacaccat attgtttcgc
    74281 cagtaatgca tacgtctggc gaacgcgaga tccctcaatt ccacgtagct gctccactga
    74341 acggcgagct ggcggtggct cacgaaaacg taattcatac attttgcgca ccaccttcag
    74401 acgtagatct tccgttaaag ccagctttgc ctggtagagt aacttatcag cacgcgctcc
    74461 accgggttgt ccggaagagt agacgcgaac acccgcttca ccgacccaga cctgtagcgt
    74521 ccccaccgtg gcggccagat gcaccgccgc gtgggaaact ctcgttcccg gttcgagcat
    74581 aatgcaggcg accgatccca ccggaatgtg cgtgcggatc ccggttttat cgatcagcac
    74641 gaaagcgccg tccagtacgt cgatttgccc gtactggagg aagatcatag aggtgcgatc
    74701 ttttaacggg atcgggctca gcggtacaaa cgtcacgcct ctgctccggg tttgatcagc
    74761 atcagaccac agccgaacgc gcggcttttg ccatacccct gactgagacg ttgcagaaat
    74821 aacccgggat cggtgaccgt aagcatcccc gtataatcca cgctactgaa ctggatcagt
    74881 tgccgggagt tttcccgccg cagttgctgt tgtcggtagg catcaaccga gatatccagc
    74941 agtgtaaaac cacttctctc cccctgcgct gccagccagt ccagcgccgc ctgttgttga
    75001 tgcaaccaga catcacttcc ttccgcctgc cccctcacct gccgtttcgc ctccatcagc
    75061 agatcgtggc gcttgcccgc tttacagatt gttggattcg cccgcaggtt gaaacaaagt
    75121 tgttgtccgg tacacagctc gggcgcaaat gaccggcatt ctatggtgaa cgtatcactt
    75181 tccgccgggc gttcctgcga cagtacaaaa aagcgaaacg cgccctggag ttcttcccga
    75241 cgataaagaa attgcctttc tttgccgcta gggaagagat cccacagcca ctgatgcatc
    75301 acatattcac cgcgatccac cagatgcagc aactgcgcag gcgaaagctg gccggtatgt
    75361 aaggttattc ttgagaggta catggttcct ccttgctgag ccacggcccc tgattgacgg
    75421 tgcgttcccc aaacagccac tgctgacgat ttaaaggaac atcccggcgg cgtaatattt
    75481 tgctcgcgac caggccgtcg tgtttccctt cccaccagca ttcatcctga agtttcggga
    75541 gtgaaacttt cagtttatgg aaatgatcct gatactgttg gtatgcatta cataaggcat
    75601 cacacgcgtt gccttcgagc agtaacggcg caagaggtaa tgccagcgga tgactttttc
    75661 gccccagata aagcggaaaa actggatggc gtaaaccgtc ctgcaactgt tcgaggctgt
    75721 aaggcgcatc ggctgttgtt gccaccgcca ccatccacca ggcatcggtg tagtagtcgc
    75781 gccgggagat aatcgcgctc aaaagctcag ggtcgctcaa ctcttcgcga cggctgaaat
    75841 aacgtgcttt acgcacctct tttggcatct ggaccgtgtg ataatcccgt gcccagcgcg
    75901 ggttacggct ggcgcaaacc accagtgaat agtggcgatt aaacgcgttt aagcgttcgg
    75961 tatcatcacg ccggatccct accccggcag ccagcagccc cagcaatgct gagagcgaag
    76021 gcagttcatg ggtatgacgc acttcgccgg gggcatcgac gccccaggat gccatcggcc
    76081 catgaagctg aaaaatcaga tattggctca taagccgccc cttacgcgca gataaagtcc
    76141 agcacgtcct tcatgcttcc ctgtttgttc atcacgtcaa aacttgcgta atcggtcttc
    76201 tgttcgtaga ccgtattcat attttcgcga agagttgtaa tacgctgcac cgccacctct
    76261 aactgacggg tgccattaat gggttcatag aaagctgccg ccagagaacg cggttgatcg
    76321 gtgccttttt ctgccagcgc ccaggaggca taagcccggc tggcaaagct gttttgttta
    76381 ccggttgggg agactttaag cgcggcttcc gtaaaggcgc gtatggtctg attagctaac
    76441 gcttcgtcac cgccgaggtt ttctaccagc agatctttat caatgcagat aagatatagg
    76501 tgtagaacag cgcagaaccg aatccggttt catctacatg accggcaccg gcatcttcag
    76561 aagcctggcg taaatcatct acggcggtga aaaaatcatt ttcgacaatc gtttcactga
    76621 caccaaacgc atgcgcgacc tggcaggcgg cttctacatt aaacccgggt tttttcgcca
    76681 gcatacgacc aaacatagcg atatctaccg ccatacggtc tttacgtaac aaagcgagat
    76741 ctttctcttt tggcgcgcgc ttttcttctg ccagttgatg ggccagcgct tttacggcgt
    76801 caaattctgc cgggctgata tggacgagct gttcagtttc ggcgctagtg agcggatctt
    76861 ttggtttttt gtcgtttttt tgttttttgt cttttttggc tttcccaaga taatcggcta
    76921 tctctactgc ccattcgatg gcttttttat cttcgattcc tttctcaatc aggatagttg
    76981 ccgcctcacg cgcaatacgc ccgctacgaa caccaatatg gcccgccagc gcctgttcaa
    77041 aaagttcaga agtgcgccaa gcacgcttca gactttgcga ggaaacgcgc agacgcgttg
    77101 ctccacccag aatcacagtt ttcggagagc cggaatcatc acggttaaga ttggcagcag
    77161 ggtaagcggt taacaaatga agctgaataa atgttgtcat gagatagtcc tttattaatt
    77221 ttgttcgtta tcggcgtcgc cagcctggta atattccagc gcccagcgcc cagcgcccag
    77281 cgcccagcgg atacggataa attctgttgg tcgctgctgg cggcggtgat gattgagcag
    77341 gtcatcgctc tcctggcacc agcggaagac cccctcttcc agagagtcaa gattgacgga
    77401 accgtttaat aatttgaccg cacggcgtaa ctgccgcaat aaatcatccg gcgtttttac
    77461 tgccaacaaa cgagtaaacc gccccttttt cattaccgcc gccagttgcg cggcaaaagg
    77521 ctgccgttcg tcaatagctt taacattggc gctaactgct gctatcagcg ctagtgcggt
    77581 gatacgccac tcgggttcat cctgccactt tatttgtctg ttcttaagaa acaggcgaaa
    77641 tccatccgtc agacaaacat cattaaccgt cgtactacgc cgcagactgg cacgctcgcc
    77701 gcgtttatcc tgcaactctt catgccattt acgcagcgca actttatgct catcttttac
    77761 aatactcatt cagcagcctc ctgctttttc tccctggcgg cgttagcatt ttgcttctcc
    77821 gctgatgttg taaaatattt cttgcgtgcg gtcatgacgc gttccaaatc aacgggctca
    77881 tatggattgg taaagacacg atcgtcaaaa tcctgacgcg cgaataacca catttccttt
    77941 tgccatttac tgagtaattc gtccggatcc tgaccttctt caattttgcg cactaatctc
    78001 aggaatcgat gctgagtttt gttccagaag tcaatatcca caaaaccgaa atcaagcttt
    78061 gtggttttct tctcagagaa ccatgcttct ttcaacgctc ggtgtaacaa gcttagaatg
    78121 tgtgatgccg cttgcgctgc cagacgcaac ttaggtatta ggtcttcttt tttgtttaac
    78181 aacagcggga aatggtgttc ataccaacaa cgaaccttca tatcttcaaa atcaaaacca
    78241 aatccccata agcccaccct tgtctgcttt agattgctgg cattcaaaag tttcacgacc
    78301 tgtgcgggga gttcagtatt gttttttgag ttacctacct ctatgaggcc taaccagtcg
    78361 cgccagatta aaccaccagg ttgtggttta actgagtaaa aatctccacc ctctttaagc
    78421 ggcagacggt aaggtgttag aggatggcgc cacatgacgt attgaacacc atagtttttg
    78481 agtgacatca agcccaaaag agcatcgctc tgcctaccgc aaatgtcgca gttacctatt
    78541 gaggttgtct taaaatcaat acgtatacgt cgtggcattc cccagtaggc ctgaagttta
    78601 ttgacttgtt catccgttac cacagcaccg tccagttcgc tggtacgcgt cggtgcaagc
    78661 caaggaaaga ccaggtcatc aaaggttttt ggtaaaggta aatcggcttc atcttgcggc
    78721 attacgttta gccacagctt tcgccacaaa ggggtttgtt gattgccctg atactccagc
    78781 aattctataa gtgtcgtcag aggccctccg ccacgtagcc caggatagta accgcgtcca
    78841 cctataggtg cattcaactg gattgaaaaa agcgctaaag gcacacaatg caggcacaga
    78901 tgctgagttg tgtctcgctt aataaaatgg tctgtatttc gttcttttgt ttgttttcct
    78961 ggtgcgtcag gaagaagcga agcaataaaa gttgccttaa cttttaattc atcaaaatcc
    79021 tgcataaatg aaggggaatc tggcccaaac tgaaaagcat gttctaaaga cagcaatgct
    79081 tcccttagct tttcagcttc cagtccgtct tcccagatat catcccaact acgttgatct
    79141 tttggcgcga agctggtttg tagtagcccc agcaaaaact gccaggccgc cccctggaga
    79201 tctgcccgcg gcgcagcgat atcgacaaca ttttcatcag ccagatcgac tggtgccagt
    79261 ttgcctgtcg ttccgtcttt aaaacgaacg ggcaaccacg gggttgtcag aagtgaaaac
    79321 gagttcatac gcatcctctc gtaaaaaaga ccatcccagg tcggttgtcc atccttccat
    79381 ccttttctgt atatcaacta ttcagagagt cttgtcgcag acatctcaac caaccttgcc
    79441 aatcaatccc tccctggccg aatacccgca actttcgtca tcggtgacta aaatcacgtt
    79501 tgccatttcc ggatcttgcc gctgttcaac gcaccactgc ctgaacgcat ccccttccag
    79561 taaagaaaac tcatcccgat gttttttcca ccagcttcgg cgcactctga caacgctcat
    79621 ttcccatgcg tgagcaccgg tggcataagg cttcaccaca tcgtcaatac aggtagccag
    79681 ccacagggaa acagactctt cagccagacg tgtcgacagt ttttccggaa ggtaatcgtt
    79741 gatattggcg gcatagccga gcctgaagtt cagcacatac tttttagccc ttgcgcgatc
    79801 acagtaatat ttgcccactt gctcctgctc gctgcgggca aatccttccg gcattaccac
    79861 gtcctcaccg taaactgatt caataagaag ccgggctgcg tgcggcatct gaatagcgcc
    79921 ttgctcacgc agcacacgct gcgtcagcca gattcgccca tgatcgggat agacatatgc
    79981 actgttacgc atggcactgc cgaaccattc gtcaccagga gcgtcatccc agacgggggc
    80041 cagaatcagc aattcagggg gggaacgctc gtcttttccg tcacgcttta actgaccatt
    80101 aatatcgcgg atatgccgct gtaatcgccc cgctcgctgg attagcaaat caatgggggc
    80161 caggtcggag atcatttcgt ccaggtcaat atccacgctt tgttcaatta cttgtgtaca
    80221 aacaatgact tgagacgctc tctgtaaact acaatattta ccaaagcgtg ccagcgtttc
    80281 cgtttcaatt cgctggcgat cgctaaaagc aaagcggcta tgaaaaagtg aaaggctgga
    80341 agcaggaatg acgccgcggg caagcagctg acgatacacc tgaatagcgt catcaacaga
    80401 attccgaatc caggcgatgc atttcccctg acttaccgcc gattcgatac gcgcaataca
    80461 ctcttgttca ctatggagcc aacccacgct cacgctacgt tcaacttctt tgcgcgtcgc
    80521 tacccggtgc gaatgtacat cggttttcgt gacatgcgtc agccaggggt aatcatcttt
    80581 tcccaggagt ggggcttctt gctggccctc tgcaccacgc gcaaaggcgg cgacgagttt
    80641 gtcgcgctgc tgttgagata acgtagcaga aagcaaaata acgctgtttc cgccacgcgc
    80701 ctgccgctcg atcagccctt caagaatgca cgacatataa gcgtcacagg cgtggatctc
    80761 atcagccagc aggattttgt tactcagccc cagaagccgc agattattat gtttaaacgg
    80821 catcactgcc atcatcgcct gatccagggt gccgacgcca atttcagcca gcaacgcctt
    80881 cttgttactg tcggcaaact aggcc
//
LOCUS       DAJTNN010000003        66148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTNN010000003 DAJTNN010000000
VERSION     DAJTNN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.003"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..407)
                     /gene="folK"
                     /locus_tag="OO150_000179"
     CDS             complement(<1..407)
                     /gene="folK"
                     /locus_tag="OO150_000179"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5514455.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPEL"
     gene            complement(404..1822)
                     /gene="pcnB"
                     /locus_tag="OO150_000180"
     CDS             complement(404..1822)
                     /gene="pcnB"
                     /locus_tag="OO150_000180"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5514456.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1861..2787)
                     /gene="gluQRS"
                     /locus_tag="OO150_000181"
     CDS             complement(1861..2787)
                     /gene="gluQRS"
                     /locus_tag="OO150_000181"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5514457.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(2824..3279)
                     /gene="dksA"
                     /locus_tag="OO150_000182"
     CDS             complement(2824..3279)
                     /gene="dksA"
                     /locus_tag="OO150_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5514458.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3457..4161)
                     /gene="sfsA"
                     /locus_tag="OO150_000183"
     CDS             complement(3457..4161)
                     /gene="sfsA"
                     /locus_tag="OO150_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5514459.1"
                     /translation="MEFSPLLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4176..4706)
                     /gene="thpR"
                     /locus_tag="OO150_000184"
     CDS             complement(4176..4706)
                     /gene="thpR"
                     /locus_tag="OO150_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5514460.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4780..7209
                     /gene="hrpB"
                     /locus_tag="OO150_000185"
     CDS             4780..7209
                     /gene="hrpB"
                     /locus_tag="OO150_000185"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5514461.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQSPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7405..9927
                     /gene="mrcB"
                     /locus_tag="OO150_000186"
     CDS             7405..9927
                     /gene="mrcB"
                     /locus_tag="OO150_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5514462.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWAGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10147..12336
                     /gene="fhuA"
                     /locus_tag="OO150_000187"
     CDS             10147..12336
                     /gene="fhuA"
                     /locus_tag="OO150_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5514463.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12387..13184
                     /gene="fhuC"
                     /locus_tag="OO150_000188"
     CDS             12387..13184
                     /gene="fhuC"
                     /locus_tag="OO150_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5514464.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSSKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13184..14074
                     /gene="fhuD"
                     /locus_tag="OO150_000189"
     CDS             13184..14074
                     /gene="fhuD"
                     /locus_tag="OO150_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5514465.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14071..16053
                     /gene="fhuB"
                     /locus_tag="OO150_000190"
     CDS             14071..16053
                     /gene="fhuB"
                     /locus_tag="OO150_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5514466.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATSTMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16088..17368)
                     /gene="hemL"
                     /locus_tag="OO150_000191"
     CDS             complement(16088..17368)
                     /gene="hemL"
                     /locus_tag="OO150_000191"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5514467.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17593..19014
                     /gene="clcA"
                     /locus_tag="OO150_000192"
     CDS             17593..19014
                     /gene="clcA"
                     /locus_tag="OO150_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5514468.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSEILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19038..19103
                     /locus_tag="OO150_000193"
     CDS             19038..19103
                     /locus_tag="OO150_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514469.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19096..19440
                     /gene="erpA"
                     /locus_tag="OO150_000194"
     CDS             19096..19440
                     /gene="erpA"
                     /locus_tag="OO150_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5514470.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19487..20110)
                     /locus_tag="OO150_000195"
     CDS             complement(19487..20110)
                     /locus_tag="OO150_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5514471.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20148..20947)
                     /gene="btuF"
                     /locus_tag="OO150_000196"
                     /pseudo
     CDS             complement(20148..20947)
                     /gene="btuF"
                     /locus_tag="OO150_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
     gene            complement(20940..21638)
                     /gene="mtnN"
                     /locus_tag="OO150_000197"
     CDS             complement(20940..21638)
                     /gene="mtnN"
                     /locus_tag="OO150_000197"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5514472.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFEADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21722..23239
                     /gene="dgt"
                     /locus_tag="OO150_000198"
     CDS             21722..23239
                     /gene="dgt"
                     /locus_tag="OO150_000198"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5514473.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23369..24793
                     /gene="degP"
                     /locus_tag="OO150_000199"
     CDS             23369..24793
                     /gene="degP"
                     /locus_tag="OO150_000199"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5514474.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24948..26105
                     /gene="cdaR"
                     /locus_tag="OO150_000200"
     CDS             24948..26105
                     /gene="cdaR"
                     /locus_tag="OO150_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR5514475.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGRLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIYRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26194..26580)
                     /locus_tag="OO150_000201"
     CDS             complement(26194..26580)
                     /locus_tag="OO150_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5514476.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26742..27554)
                     /gene="yaeI"
                     /locus_tag="OO150_000202"
     CDS             complement(26742..27554)
                     /gene="yaeI"
                     /locus_tag="OO150_000202"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR5514477.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27609..28433)
                     /gene="dapD"
                     /locus_tag="OO150_000203"
     CDS             complement(27609..28433)
                     /gene="dapD"
                     /locus_tag="OO150_000203"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5514478.1"
                     /translation="MQQLQNIIETAFERRAEITPTNADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGHVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28464..31136)
                     /gene="glnD"
                     /locus_tag="OO150_000204"
     CDS             complement(28464..31136)
                     /gene="glnD"
                     /locus_tag="OO150_000204"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5514479.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31198..31992)
                     /gene="map"
                     /locus_tag="OO150_000205"
     CDS             complement(31198..31992)
                     /gene="map"
                     /locus_tag="OO150_000205"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5514480.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32360..33085
                     /gene="rpsB"
                     /locus_tag="OO150_000206"
     CDS             32360..33085
                     /gene="rpsB"
                     /locus_tag="OO150_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5514481.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33343..34194
                     /gene="tsf"
                     /locus_tag="OO150_000207"
     CDS             33343..34194
                     /gene="tsf"
                     /locus_tag="OO150_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5514482.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34341..35066
                     /gene="pyrH"
                     /locus_tag="OO150_000208"
     CDS             34341..35066
                     /gene="pyrH"
                     /locus_tag="OO150_000208"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5514483.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35358..35915
                     /gene="frr"
                     /locus_tag="OO150_000209"
     CDS             35358..35915
                     /gene="frr"
                     /locus_tag="OO150_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5514484.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36007..37203
                     /gene="ispC"
                     /locus_tag="OO150_000210"
     CDS             36007..37203
                     /gene="ispC"
                     /locus_tag="OO150_000210"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5514485.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37391..38149
                     /gene="ispU"
                     /locus_tag="OO150_000211"
                     /gene_synonym="uppS"
     CDS             37391..38149
                     /gene="ispU"
                     /locus_tag="OO150_000211"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5514486.1"
                     /translation="MLSATQLLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFIDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38162..39019
                     /gene="cdsA"
                     /locus_tag="OO150_000212"
     CDS             38162..39019
                     /gene="cdsA"
                     /locus_tag="OO150_000212"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5514487.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVSLLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39031..40383
                     /gene="rseP"
                     /locus_tag="OO150_000213"
     CDS             39031..40383
                     /gene="rseP"
                     /locus_tag="OO150_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5514488.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40413..42845
                     /gene="bamA"
                     /locus_tag="OO150_000214"
     CDS             40413..42845
                     /gene="bamA"
                     /locus_tag="OO150_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5514489.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42967..43452
                     /gene="skp"
                     /locus_tag="OO150_000215"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42967..43452
                     /gene="skp"
                     /locus_tag="OO150_000215"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5514490.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43456..44481
                     /gene="lpxD"
                     /locus_tag="OO150_000216"
     CDS             43456..44481
                     /gene="lpxD"
                     /locus_tag="OO150_000216"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5514491.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44586..45041
                     /gene="fabZ"
                     /locus_tag="OO150_000217"
     CDS             44586..45041
                     /gene="fabZ"
                     /locus_tag="OO150_000217"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5514492.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45045..45833
                     /gene="lpxA"
                     /locus_tag="OO150_000218"
     CDS             45045..45833
                     /gene="lpxA"
                     /locus_tag="OO150_000218"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5514493.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            45833..46981
                     /gene="lpxB"
                     /locus_tag="OO150_000219"
     CDS             45833..46981
                     /gene="lpxB"
                     /locus_tag="OO150_000219"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5514494.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVARDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46978..47574
                     /gene="rnhB"
                     /locus_tag="OO150_000220"
     CDS             46978..47574
                     /gene="rnhB"
                     /locus_tag="OO150_000220"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5514495.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47611..51093
                     /gene="dnaE"
                     /locus_tag="OO150_000221"
     CDS             47611..51093
                     /gene="dnaE"
                     /locus_tag="OO150_000221"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5514496.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGVNAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51106..52065
                     /gene="accA"
                     /locus_tag="OO150_000222"
     CDS             51106..52065
                     /gene="accA"
                     /locus_tag="OO150_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5514497.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52164..54273
                     /gene="ldcC"
                     /locus_tag="OO150_000223"
                     /pseudo
     CDS             52164..54273
                     /gene="ldcC"
                     /locus_tag="OO150_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            54330..54719
                     /locus_tag="OO150_000224"
     CDS             54330..54719
                     /locus_tag="OO150_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5514498.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54784..56082
                     /gene="tilS"
                     /locus_tag="OO150_000225"
     CDS             54784..56082
                     /gene="tilS"
                     /locus_tag="OO150_000225"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5514499.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56131..56391)
                     /gene="rof"
                     /locus_tag="OO150_000226"
     CDS             complement(56131..56391)
                     /gene="rof"
                     /locus_tag="OO150_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5514500.1"
                     /translation="MLMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56378..56578)
                     /locus_tag="OO150_000227"
     CDS             complement(56378..56578)
                     /locus_tag="OO150_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5514501.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56744..57289
                     /locus_tag="OO150_000228"
     CDS             56744..57289
                     /locus_tag="OO150_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5514502.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57286..57708
                     /gene="arfB"
                     /locus_tag="OO150_000229"
     CDS             57286..57708
                     /gene="arfB"
                     /locus_tag="OO150_000229"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5514503.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSAKAMRGKVRSGRE"
     gene            57722..58432
                     /gene="nlpE"
                     /locus_tag="OO150_000230"
                     /gene_synonym="cutF"
     CDS             57722..58432
                     /gene="nlpE"
                     /locus_tag="OO150_000230"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5514504.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58632..59452)
                     /locus_tag="OO150_000231"
                     /pseudo
     CDS             complement(58632..59452)
                     /locus_tag="OO150_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(59505..61223)
                     /gene="proS"
                     /locus_tag="OO150_000232"
     CDS             complement(59505..61223)
                     /gene="proS"
                     /locus_tag="OO150_000232"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5514505.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61334..62041)
                     /gene="tsaA"
                     /locus_tag="OO150_000233"
     CDS             complement(61334..62041)
                     /gene="tsaA"
                     /locus_tag="OO150_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5514506.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62038..62442)
                     /gene="rcsF"
                     /locus_tag="OO150_000234"
     CDS             complement(62038..62442)
                     /gene="rcsF"
                     /locus_tag="OO150_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5514507.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62560..63375)
                     /gene="metQ"
                     /locus_tag="OO150_000235"
     CDS             complement(62560..63375)
                     /gene="metQ"
                     /locus_tag="OO150_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5514508.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63415..64068)
                     /gene="metI"
                     /locus_tag="OO150_000236"
     CDS             complement(63415..64068)
                     /gene="metI"
                     /locus_tag="OO150_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5514509.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64061..65092)
                     /gene="metN"
                     /locus_tag="OO150_000237"
     CDS             complement(64061..65092)
                     /gene="metN"
                     /locus_tag="OO150_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5514510.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65280..65852
                     /gene="gmhB"
                     /locus_tag="OO150_000238"
     CDS             65280..65852
                     /gene="gmhB"
                     /locus_tag="OO150_000238"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5514511.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15693 a        16410 c        18174 g        15871 t
ORIGIN      
        1 agctccggtg caatctcaaa cagcggccac agcataaatc cacgattctt catatcgtag
       61 tgcggaacgg tcaggcgttc ggtatttatc acttcattac caaacagcat gatatcgaga
      121 tccagcgtgc gtggtcccca gcgttcagct ttacgaacac gatcctgttg cagttctatg
      181 cgctgtgtat ggttgagtaa ctcttccggg gcgagcgaag tttcaagcgc cacggcggcg
      241 ttgaggtaat cgggttgatc ttgcggcccc agcggtggag tgcggtaaaa cgaagaaacg
      301 gcaagaatgt ggctttcagg gatatcgcct aatgctttca gggcagcatt gacctgctcc
      361 agcggagagg ccagattgct gcctatggca atatacgcca ctgtcatgcg gtaccctcac
      421 gacgcggtgc gcgtttgcgt ggacgacgag tacggcgacg cggtgacggc tcttcatcca
      481 actcgttaag catccctttt tgatctggtg gcgcggaaac ctggaactca ccccaccatt
      541 tcaccagacg ctgcagttca gcgttacgtt caacttcagc tcgcaaggcc aacaggtcat
      601 aagccgcacg gaacttagga tgctccagca gtttccatgc gcgtttaccc tgacgacggg
      661 acatacgcaa ctgcaactgc cagatatcgc gggttaacgt cgtcagacgt ttcgggattg
      721 ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc cagcgcgaaa gcgtcgtgat
      781 aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc cagcagtggg taccagaaca
      841 tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt atggatacgc gtatcggtat
      901 tcttcagcac ctgttcaatg atccgctcca tcgggctgtc gccattttcc gtgaagtagc
      961 gggtaatggt cgggaacagc ggctggaaca gatgatattc acacaacagc ttataggttt
     1021 cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc aaacaggcgt gccggtggga
     1081 tatcgttcag cagggtagcg aggcgaggga tcggttctgc ggtttccggg ctgatgcgca
     1141 tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg taccggatct tcacggtagc
     1201 gcgtttccgg gttaccaatc agacggataa cgccgtcctt cagatccttc atgccgccaa
     1261 cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag gctgttgata gtgaaatcgc
     1321 ggcgctgggc gtcttcttcg atggagccga aaatgttgtc gcgcagcaac atgccgtttt
     1381 gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc gtggtgtcca cggaaggttg
     1441 caacttcgat aatctccggg ccaaacatca catgagccag acggaaacgg cgacccacaa
     1501 ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt ggcgttagtg gttacgtcaa
     1561 aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc gccgccaacc agccaggctt
     1621 cgtatcccgc tttattgagc ctgtacatta ccttcagggc attttcactg atatctttgc
     1681 gggaaatagc atgctgctca cgcgggatca ccgtcacctg tggacgggcg actgcctgtt
     1741 cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa attagcgact cgggtaaaaa
     1801 tagtacacct cggtagtgtc aaacatcatt caggacaaaa aaatagcggc taatcatagc
     1861 tcagcatgac gcatttgaga atgttgaatt tacaattgcc gactcgggca cggcggtcag
     1921 cgtccagttt ttgacggctg actgaaggat ttgctcgacg ctgaaatcct gccagtgtgc
     1981 ttctacctgc tgccccagaa attgaagtgc cgcgattagt accgggcgtg gatcgccttt
     2041 cggcaacgca ggcgcatgat tctgcttgga aagtttagcg ccttgtggat taagcgccag
     2101 cggcagatga atgtaatctg gcactttcca gccaaaaagc tggtacagcg agatttgcct
     2161 tactgttggt tcaatcagat cagccccacg cactatttct gtaacgccct ggaaatgatc
     2221 atcaaccaca acagccaggt tgtaggcgaa caacccatca cggcgatgaa tgataaaatc
     2281 ttcccgtgcc agtttttcgt cggcgtgaat aatgccgcgc agctggtcag taaattgcgt
     2341 gaccggatgc tgctggcgga tacgcactgc ggcgttgtct ggtccatgat gcaacacccg
     2401 gcaatgaccg tcgtaaatac cgacaatgct ttgaatacgc gcacgcgtac aggtgcagta
     2461 ataacttagt ccttgttcat gtaaccaggc gagtgcttca cgataggcgt cgtgacgttg
     2521 cgattgccag agaacatcgc cgtcccagtg cagaccgtaa tgttccagct ggcgcaggat
     2581 agtttctgcg gcaccgggaa cttcacgagg cgggtcgata tcttctatgc gtaccagcca
     2641 gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc gcggcgatca gagagccaaa
     2701 atgaagctcg ccggaaggag agggggcgaa gcggccaata tactgtgtgt ctgtcatctc
     2761 tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg taaacgtgat ggaacggctg
     2821 taattagcca gccatctgtt tttcgcgaat ttcagccagc gttttgcagt cgatgcacag
     2881 atcggctgtc gggcgcgctt ccagacggcg aataccaatt tcaacaccgc aggattcgca
     2941 gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc tcgatctttt tgatcagctt
     3001 acgctcgcga tcgcggttac gcagttcgag gctgaactct tcttcctggg ctgcacggtc
     3061 taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt gtaacggtgc gatcgacttc
     3121 atccctgagt tgattacgcc atgcttccag aatacgacgg aagtgcgcca gctgggcttc
     3181 attcatatac tcttcgcccg gcttctcctg atatggttcc accccagcga tggcgagaat
     3241 actcagggac gatgttttac ggttttgccc ttcttgcatg ttgcttctcc ttaacacgca
     3301 ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat aaatagcaga tgcttttccg
     3361 gatagcaatt atctaaacgt aacacttgac aactgtgtga ggaaaagcgt atttgcgcac
     3421 gcgaccagaa tgtaaattaa ccagttactt actttactac aatgtaaccg gcagtgattt
     3481 tttaagagcc atgccttcag cagaaatttc cgctttgtaa gccagaattt ctacccccct
     3541 ctgttgagct tctgacaata gttgcgcgta tttctcatcg atgtggcgcg cgggtgaaaa
     3601 ccgtgtaatg gctgaatgca gcacggcgaa aaagataacc gcacgctggc cttcagccgc
     3661 tacgttcatc aactcccgaa ggtgtttctg acctcgttca gtgaccgcat cgggaaaata
     3721 tccctgttcg ttctccgcta acgtaaccga tttcacttca atatagcagt ctggacgcga
     3781 atccgcctgc aacataaagt caatacggct gcgttctgcg ccatatttta cttcgctttt
     3841 cagcgagcta tagcctgaca gttctgaaat tgattcatta aggatagcct ctttcgtcaa
     3901 cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg ctctggcttt gagttagttc
     3961 ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc gaataccaga cggtatcgcc
     4021 acgcgttgca caaccggtca tcgcacccgt attcgggcag tgtagcgtta attcgcgacc
     4081 atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc tgaattagcg tcgcgcgctg
     4141 tagaagggga gagaattcca ttgcgacaat ccttgttatt gcattagcgc ccagcgtttt
     4201 agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg cgtaaagggt gaactccgtc
     4261 accgcataag accagttaaa acctggcggc gggattgtca ccgcctcgct ggcgtcgcgc
     4321 aataaggtaa tatgtggatg aaacggacga ttgctttgaa aacaaccgct gcgggcagcc
     4381 tgtgaacgga gcatattcgc cagctggatt aagccgcgtg gcggctgacg catccctaac
     4441 cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa gcgtgagtgt gaaaccaggt
     4501 tgacgaatcc gtccggctaa aagagaaagc gccttctctt tctcggcgct cacttcgcct
     4561 aaaaatgcca gagtcagatg caaattatcg gcggcgaccg gacgtcccgc ctcaggtggg
     4621 aagtgtgtgg cgcgccagtg gataatctgt tcgcggattt ctgcaggtaa gtcgatagca
     4681 aagaacagac gttgcggttc agacatgtga ggcactcggt tatgaattac cgcgatgcta
     4741 caatgtggcg cgaagaatgt taaccctctg gagcgttttg tgtcgtcgtt gcccgttgct
     4801 gccgttttac ctgaattact taccgctctc gattgtgcgc cgcaggtatt attaagtgcg
     4861 ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac tgctggcgca tcccggcatt
     4921 aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg cgcgtaacgt cgcgcaacgg
     4981 ctggaggagc tgcttaacga aaagccaggc gataccgttg gctaccggat gcgtgcgcaa
     5041 aactgcgtcg ggccgaatac ccgcctggaa gtagttaccg aaggcgtgct gacgcggatg
     5101 atccagcgtg acccggagtt gagcggtgtt ggattggtga tccttgatga atttcatgag
     5161 cgtagcttgc aggcggattt ggcgttggcg ctgttactcg atgtgcaaca aggtctgcgt
     5221 gatgacctta aactgctaat tatgtcggct acgctggaca acgaccgctt gcagcaaatg
     5281 ctgccagaag cgcccgtcgt catctcagaa gggcgctcgt ttccggttga acgccgttat
     5341 ttaccgctgc ccgcgcatca gcgttttgac gatgccgttg cggtagccac cgctgaaatg
     5401 ctgcgtcagg aaagcggatc attactgtta tttttacctg gcgtcggaga aattcagcgt
     5461 gtgcaggaac aactggcttc gcgcatcggc agtgatgtat tgctctgccc gctgtatggc
     5521 gcgttgtcgc tgaacgatca gcgaaaagcg atcctcccgg caccgcaagg gatgcgcaaa
     5581 gtggtgctgg cgaccaatat tgctgaaacc agtttaacca tcgaaggtat tcgtctggtg
     5641 gtggattgtg cccaggagcg tgtggcgcgt tttgatccgc gcacggggct aacgcgactg
     5701 attactcaac gcgttagcca ggcatccatg acgcagcgtg ccgggcgcgc cgggcgtctg
     5761 gagccgggta tctgcctgca tctaatcgcc aaagaacaag cagaacgcgc tgccgcgcaa
     5821 agtgaaccgg aaatcttaca aagcgatctt tccggtttgc tgatggaatt actgcaatgg
     5881 ggatgcagcg atccggcgca gatgagctgg ctggatcaac cgccaacggt gaatctactg
     5941 gccgcgaaac gcctgttaca aatgctgggg gcgctggagg gtgaacggct tagtgcgcag
     6001 gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag cggcaatgct ggttagcgcg
     6061 aagaacgacg acgaagctgc taccgcggca aaaattgccg ccattctcga agagccgcca
     6121 cggatgggca atagtgacct gggcgtggcg ttttcgcgca atcaaccagc ctggcagcaa
     6181 cgtagtcagc aactgttaaa acgcttaaac gtacgtggcg gtgaggcaga cagttcgctt
     6241 atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg ctcgtcgccg tgggcaagat
     6301 ggacgctatc aactggcaaa cggcatggga gcgatgctcg atgccgacga cgcgctaagc
     6361 cgccacgaat ggttgatcgc accgttatta ttgcagggca gcgcctcgcc ggatgcgcgg
     6421 attttactgg cgctgccggt cgatattgat gagttagtac aacgctgccc gcagctggta
     6481 cagcagtctg acactgtgga gtgggatgac gcgcaaggta cgctgaaagc ctggcgtcgg
     6541 ctgcaaatcg gtctgctgac ggtgaaagtg cagccgctgg cgaaaccctc ggaagacgag
     6601 ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt taagcgtgct caactggacg
     6661 gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg caaagtggtt gccggaatat
     6721 gactggccag cggttgatga tgaaagttta ttggcgacgc tggaaacgtg gctgctgcca
     6781 catatgactg gcgtacattc actacgcggc ctgaaatcac tcgacattta tcaggcacta
     6841 cgcggattac ttgattgggg aatgcagcaa cgtctggata gtgaattgcc tgcgcattac
     6901 actgtgccga cgggaagccg gatcgccatt cgttatcatg aagataaccc gcccgcgctg
     6961 gcggtgagaa tgcaagagat gtttggcgag gccaccaatc cgacgatcgc ccaggggcgc
     7021 gtgccgctgg tgctggagtt gctttcacct gcccaaagtc cattacaaat cacacgagat
     7081 ttgagcgcct tctggaaagg agcgtaccgt gaggtgcaaa aagagatgaa agggcgttat
     7141 cccaaacatg tctggccgga cgacccggca aatactgcac cgacgcgacg gacgaaaaag
     7201 tattcgtaag gttggttttc tccctctccc tgtgggagag ggccggggtg agggcatcag
     7261 cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg agaggggacc gatcgaacac
     7321 aaattttgag agatatcttc ttctgtcttg taacagaaga acagaaaatc gggcttttgc
     7381 gcctgaatat tgcggagaaa aagcatggcc gggaatgacc gcgagccaat tggacgcaaa
     7441 gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc gtcgttacga agatgacgat
     7501 gattacgacg attatgatga ctatgaggat gaagaaccga tgccgcgcaa aggtaagggc
     7561 aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc tatggctact gctaaaactg
     7621 gctatcgttt ttgccgtgct gatcgccatt tacggcgttt atctcgatca aaaaattcgt
     7681 agccgtattg atggcaaggt ctggcaactg cctgcggcag tttatggccg aatggtcaat
     7741 cttgagccag atatgaccat cagcaagaac gagatggtga agctgctgga ggcgactcag
     7801 tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta ccgtgcaggc caacagcatt
     7861 gagatgatcc gccgtccgtt tgatttcccg gacagtaaag aagggcaggt gcgtgcgcat
     7921 ctgacctttg atggtgatca tctggcgacc atcgtcaata tggagaacaa ccgtcagttc
     7981 ggctttttcc gtcttgatcc gcgtctgatc accatgatct cctcgccaaa cggtgagcaa
     8041 cgtctgtttg tgccgcgcag tggtttccct gatttgctgg tggatacttt gctggcgaca
     8101 gaagaccgcc atttctacga gcatgatggg atcagcctct actcaattgg gcgtgcggtg
     8161 ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga gtacgctgac gcaacagctg
     8221 gtgaaaaacc tgttcctctc cagcgagcgc tcttactggc gtaaagcgaa cgaagcctac
     8281 atggcgctga tcatggacgc gcgttacagc aaagaccgta ttcttgagct gtatatgaac
     8341 gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg gcttcccgct ggcaagcttg
     8401 tattactttg gtcgcccggt agaagagcta agcctcgacc agcaggcgct gttagtcggt
     8461 atggtgaaag gggcgtccat ctacaacccg tggcgtaacc cgaaactggc gctggagcga
     8521 cgtaatctgg tgctgcgtct gctgcaacag caacagatta ttgatcaaga actctatgac
     8581 atgttgagtg cccgtccgct gggggttcag ccgcgcggtg gggtgatctc tcctcagcca
     8641 gcctttatgc aactggtgcg tcaggagctg caggcaaaac tgggcgataa ggtaaaagat
     8701 ctctccggcg tgaagatctt cactaccttt gactcggtgg cccaggacgc ggcagaaaaa
     8761 gccgccgtgg aaggcattcc ggcactgaag aaacagcgta agttgagcga tcttgaaact
     8821 gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg cgatggtggg aggttctgag
     8881 ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc gttcgattgg ttcccttgca
     8941 aaaccagcga cttatctgac ggctttaagc cagccgaaaa tctatcgcct gaacacgtgg
     9001 attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc tggtctggtc accgcagaac
     9061 gatgaccgtc gttatagcga aagcggcaga gtgatgctgg tggatgcgtt gacccgttcg
     9121 atgaacgtgc cgacggtaaa tctggggatg gcgctggggc tgcctgcagt tacggatacc
     9181 tggatcaaac tgggcgtacc gaaagatcag ctgcatccgg ttccggcgat gctgctgggg
     9241 gcgttgaact taacgccaat cgaagtggcg caggcgttcc agaccatcgc cagcggtggt
     9301 aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg aagatggcaa ggtgctgtat
     9361 cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg cggcgtatct gacactatgg
     9421 accatgcagc aggtggtaca acgtggtacg ggtcgtcagc ttggggcgaa atacccgaac
     9481 ctgcatctgg cagggaaaac agggactacc aacaataacg tagatacttg gtttgcaggc
     9541 attgacggca gcacggtgac catcacctgg gcaggccgtg ataacaacca gccgaccaaa
     9601 ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt atctggctaa ccagacgcca
     9661 acgccgctga atcttgttcc gccagaagat attgcagata tgggcgtgga ctacgacggc
     9721 aactttgttt gcagcggtgg catgcgtgtc ttgccggtct ggaccagcga tccgcaatcg
     9781 ctgtgccagc agagcgagat gcagcagcag ccgtcaggca atccgtttga tcagtcttct
     9841 cagccgcaac agcaacctgc tcagcaagag cagaaagaca gcgacggtgt agccggttgg
     9901 atcaaggata tgtttggtag taattaacat ctaagcgtga aataccggat ggcgagttgc
     9961 catccggtaa aataacatcc catctaagat attaaccctt tcttttcatc tggttgttta
    10021 ttaacccttc aggaacgctc agattgcgta ccgcttgcga acccgccagc gtttcgaata
    10081 ttatcttatc tttataataa tcattctcgt ttacgttatc attcacttta catcagagat
    10141 ataccaatgg cgcgttccaa aactgctcag ccaaaacact cactgcgtaa aatcgcagtt
    10201 gtagtagcca cagcggttag cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa
    10261 gaagacacta tcaccgttac cgctgcacct gcgccgcaag aaagcgcatg ggggcctgct
    10321 gcaactattg cggcgaagca ctctgctact gcgactaaaa cggatacacc aattgaaaaa
    10381 acgccgcagt ctatttcggt tgtgacaaat gaagagatgc agatgcatca atttcagtct
    10441 gtaaaagaag cattaggtta tacacctggc gttacggtta gcagtcgtgg tgcttctaat
    10501 acatatgatt ttgttatcat tcgtggtttc tcatctgttg gtctgaatca aaataattac
    10561 cttgatggac taaaacttca gggcaacttt tataacgatg ctgtgattga tccttacatg
    10621 ctcgagaggg ttgaactgat gcgtgggccg acgtctgttc tctatggaaa aagtaatcct
    10681 ggtgggatta tttctatggt gagtaagcgt ccgaccactg aacccctgaa agaaattcag
    10741 tttaaaatgg ggacggataa tctgtttcag accggattcg atttcagtga tgcactggat
    10801 gataacggtg agttctctta ccgtttgacg ggcctcgctc gctcaacaaa cgaacagcag
    10861 aaaaactctg aatctcagcg ttataccatt gctccatcat tctcatggcg tccagacgac
    10921 aaaaccaatt ttactttcct gtcctatttc cagaatgaac cagaaacggg ttattacggt
    10981 tggttgccga aagaggggac cgttgagcca ttgcctaatg gtaagcgtct accgactgac
    11041 ttcaatgaag gtgcatcgaa taatacatac tcccgtaacc agaaaatggt gggatatagt
    11101 tttgaacatg gttttaatga caccttcacc gtgcgtcaga atctgcgttt cagtgaaatg
    11161 aaaacctcac agaaaagtgt ttatggcaca gggattgctg gtgatggtca taccctaaac
    11221 cgcgggacag tggtggataa tgagcgtcta caaaacttta gcgttgatac ccaacttgaa
    11281 agtaagtttg ctacaggtga cgtcgagcat actttgctga caggggtaga cttcatgcgg
    11341 atgcgtaatg atatcaatgc cagctttgga tccgcaccat ccatcgatct ttataacaaa
    11401 tatcaccctg aatactttgc atttggtagc gcagagccat accagatgaa tgaaagcaaa
    11461 caaacaggta tttatgttca ggatcaggcg gaatggaata aatgggtatt cactctgggg
    11521 ggacgctacg attggtctaa gcaagcgacc actgttcgtg aaaactctta tacgccgact
    11581 gaaggttata ttgagcgcaa tgatcatcag ttcacctggc gcggtggtgt aaattactta
    11641 ttcgataatg gtatttcacc ttactttagc tatagccagt cctttgaacc gagtgctttc
    11701 gatctgtgga gcaacccacg catttcctat aagccatcga aaggtgaaca gtatgaagct
    11761 ggcgtaaaat atgttccgaa tgatatgccg gtcgttgtta cgggcgcagt ctatcaattg
    11821 acgaaaacaa ataacctgac agcagaccca acaaacccat tagcgcaagt cccagcaggt
    11881 gagattcgcg ctcgtggtgt ggaacttgaa gcaaaagcgg cgttaaatgc caatattaac
    11941 ttgacggctt cttataccta caccgatgcg gaatacacca aagacaccaa tctcaaaggt
    12001 aaaactccag aacaagtacc ggagcatatg gcatctctct ggggggatta taccttcaat
    12061 gaagggccgc tttctggttt aacattggga acaggtggtc gttttattgg ttccagctat
    12121 ggtgatccgg caaacacttt taaagtgggt agcgcagctg taatggatgc tgttgtaaaa
    12181 tatgatctgg cacgctttgg tatggcggga tccagccttg ctgtgaacgt caacaatttg
    12241 ctcgatcgtg agtatgttgc cagttgcttc cagacttatg gctgcttctg gggcgcagaa
    12301 cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttc tcttttgggg cacggatttc
    12361 cgtgcccatt tcacaagttg gctgttatgc aggaatacac gaatcattcc gataccactt
    12421 ttgcactgcg taatatctcc tttcgtgtgc ccgggcgcac gcttttgcat ccgctgtcgt
    12481 taacctttcc tgccgggaaa gtgaccggtc tgattggtca caacggttct agtaaatcca
    12541 ctctgctcaa aatgcttggc cgtcatcagc ctccgtcaga aggggagatt cttcttgatg
    12601 cccagccgct ggaaagctgg agcagcaaag cgtttgcccg caaagtggct tatttgccgc
    12661 agcagcttcc tccggcagaa gggatgaccg tgcgtgaact ggtggcgatt ggtcgttacc
    12721 cgtggcatgg cgcgctgggg cgctttgggg cggcagatcg cgaaaaagtc gaggaagcta
    12781 tctcgctggt tggcttaaaa ccgctggcgc atcggctggt tgatagtctc tctggcggcg
    12841 aacgtcagcg ggcgtggatc gccatgctgg tggcgcagga tagccgttgt ctgttgctcg
    12901 acgaaccgac ctcggcgctg gatatcgccc accaggttga tgtgctggcg ctggtgcacc
    12961 gtttaagtca ggagcgtggc ctgacggtca ttgccgtgtt gcacgatatc aatatggcgg
    13021 cacgctactg tgattatctg gtcgccctgc gcggtggtga aatgattgct cagggaacgc
    13081 ctgcggaaat tatgcgcggc gataccctcg aaatgattta tggcatcccg atgggtattt
    13141 tgccgcatcc ggcgggtgct gcacctgtga gttttgttta ttgatgagcg gcttacctct
    13201 tatttcgcgc cgtcgactgt taacggcgat ggcgctttct ccgttgttat ggcagatgaa
    13261 taccgcccac gcggcggcta ttgatcccaa tcgtattgtg gcgctggagt ggttgccggt
    13321 ggaattactg ctggcgctcg gcatcgtgcc ttacggcgta gcggatacca tcaactatcg
    13381 cctgtgggtc agcgaaccac cattgccgga ctcagtgatc gacgtcggtt tgcgcacaga
    13441 acctaacctt gaactgctga ccgaaatgaa accgtcgttt atggtctggt cggcaggata
    13501 tggcccttca gcagaaatgc tggcacgtat tgcgccgggg cgtggattta acttcagcga
    13561 cggcaaacag ccgctggcga tggcgcgtaa atcactgacg gaaatggcag atttacttaa
    13621 cctgcaaagc gcagcagaaa cgcatttagc gcaatatgaa gactttatcc gcagcatgaa
    13681 accccgcttt gtgaagcgtg gtgcacgccc gttattgctg acgacgctta tcgatccgcg
    13741 ccatatgttg gtcttcggtc caaacagctt gttccaggaa attcttgatg agtacggcat
    13801 cccaaatgcc tggcaagggg aaaccaactt ctggggcagt accgccgtca gtatcgatcg
    13861 tctggcagcg tataaagacg ttgatgtgct ctgttttgat cacgacaaca gcaaagacat
    13921 ggatgcgcta atggcaaccc cgctgtggca ggccatgccg tttgtccgcg ccggacgctt
    13981 tcagcgcgta cccgcagtct ggttttatgg tgcgacgctc tcggcaatgc actttgtgcg
    14041 cgttctggat aacgccatcg gaggtaaagc gtgagtaaac gaattgcgct tttcccggcg
    14101 ttattgctgg cgctgctggt gattgtcgct acggcgctga cctggatgaa cttttcgcag
    14161 gcgctgccgc gtagccagtg ggcgcaggcc gcctggtcgc cggatattga cgtcatcgag
    14221 cagatgattt ttcactacag cttgctgccg cgtctggcga tttcgctgct ggtgggcgca
    14281 ggcctggggc tggtgggcgt gctgtttcag caagtgctgc gtaatccgct ggcggaaccg
    14341 acgacacttg gcgttgctac aggcgcgcaa ctggggatta ccgtcactac gctctgggcg
    14401 atcccagggg cgatggcgag tcagtttgct gcgctggcgg gcgcttgcgt tgttggctta
    14461 atcgtctttg gcgtcgcgtg ggggaaacgg ctttcgccgg taacgctgat cctcgcgggg
    14521 ctggtagtga gcctttattg tggggcaatc aatcagttac tggttatctt ccaccatgac
    14581 caactgcaaa gcatgttcct gtggagcacc ggaacgctga cgcaaaccga ctggggcggt
    14641 gttgagcgtt tatggccgca gttgctgggc ggtgtgatgc tgacgttgct gctacttcgc
    14701 ccgttaaccc tgatggggct tgatgatggc gtggcgcgca atctcgggct ggccttgtcg
    14761 ctcgcgcgtc tggcggcgct gtcgctggcg attgtcatca gtgcgctgct ggtgaacgcg
    14821 gtggggatta tcggctttat cgggttgttt gcgccgttgc tggcaaaaat gctgggggcg
    14881 cggcgtctgc tgccgcgact gatgctggcg tcgttgattg gtgcgctgat cctctggctt
    14941 tccgatcaaa tcatcctctg gctgactcgc gtgtggatgg aagtgtccac cggttcggtc
    15001 actgcgttga tcggtgcgcc gctgctactg tggctgttgc cgcgtttacg cagcattagc
    15061 gcgccggata tgaaggtcaa cgatcgtgtc gcggctgaac gccaacatgt gctggcgttt
    15121 gccctcgcgg gcggcgtgct gctgttgatg gctgtggtgg tggcgctgtc gtttggtcgt
    15181 gatgcgcacg gctggatgtg ggcgagcggg gcgttgctcg atgatttaat gccctggcgc
    15241 tggccgcgaa ttatggcggc gctatttgcg ggcgtcatgc tggcggtggc gggctgtatt
    15301 attcagcgac tgaccggcaa cccgatggca agcccggaag tgctggggat tagctccggc
    15361 gcagcgtttg gcgtggtact gatgctgttt ctggtaccgg gtaatgcttt tggctggctg
    15421 ttacccgcgg gaagtatcgg tgccgcggtg acgctgttga ttattatgat cgccgccggg
    15481 cgcggtggat tttccccaca ccgcatgtta ctggcgggga tggcgttaag taccgcattc
    15541 accatgcttt taatgatgtt gcaggcgagt ggtgacccgc gaatggcgca agtgctcacc
    15601 tggatctctg gttcgaccta caacgccacc gatgcgcagg tctggcgtac cggaattgtg
    15661 atggtgattt tgctggcgat taccccgctg taccgccgct ggctgaccat tttacctctg
    15721 ggcggtgata ccgcacgagc tgtgggaatg gcgctgacgc cgacgcgaat tgcgctgctg
    15781 ctgttagcgg cttgcctgac ggcgacctcg acgatgacta ttggaccgtt gagttttgtt
    15841 ggtttaatgg caccgcatat tgcgcggatg atgggttttc gacggacgat gccacacatc
    15901 gtaatttcgg cgctggtggg tggtttactg ctggtattcg ctgactggtg tgggcggatg
    15961 gtgctgtttc cattccagat cccggcgggg ctgctgtcga cctttatcgg cgcgccatat
    16021 tttatctatt tgttgagaaa gcagagccgt taattgagtg cctcgcccgg tgggcgaggc
    16081 aaaattatca caacttcgca aatacccgac gtgcagcatc gatggtgtta ttgatatctt
    16141 ccatgctgtg cgccacagac ataaagcccg cttcaaacgc tgacggtgcc aggtaaacac
    16201 cttcgtccag catcatatgg aagaaacgct taaagcgttc cacgtcacag gccatcacat
    16261 cctgatagca cgtcacggac tcggcgtcgg taaagaaaat accgaacatg ccgccaacgt
    16321 ggttaacgac cagcggagtt ccggcttctt ctgccgcttc cagcagacct tctgccagac
    16381 gtgttgtcag ctcatccagc gtttcgtgaa cgcccggctg cgcgacttca ttcagacagg
    16441 cgaaacccgc tgccatcgca atcgggttac cggaaagcgt acccgcctga tagaccggac
    16501 ccgtcggggc cagcgcatcc attacatcac gacgaccacc gaatgcgcct accggcattc
    16561 caccgccgat gattttgccg aggcaggtta aatctggcac tacgccgtaa taatcctgtg
    16621 cgccagccag tgccacgcgg aagccggtca tcacttcatc gatgatcagc aacgcgccaa
    16681 attcgtcgca cagcgcacgc agacctggca ggaactctgg ctgcggcgga acacagttca
    16741 tattgcctgc caccggctcg acgataatac aggcaatctc ttgcgggtat tgctcaaacg
    16801 cggcgcgtac agaagccaga tcgttataag tacaggttaa ggtatgtttg gcgaaatctg
    16861 ccggaacacc cggcgagttt ggctggccta acgtgagtgc gccagaaccg gctttcacca
    16921 gcaggcagtc agcgtgaccg tggtaacaac cttcaaattt aatgattttg tcgcgaccgg
    16981 taaaaccacg ggccaggcgg atggcgctca tggtcgcttc agtgccggag ttcaccatgc
    17041 gcaccatatc catggtcggg accagttcgg tcaccagttg cgccattttc acttccattt
    17101 cggtcggtgc accaaagctt aaaccacgct cggcggcttc aatcacggca ttgcggatag
    17161 ccggatggtt atggcccagc accatcggcc cccaggaacc gacataatcg atataggctt
    17221 tgccatcaac atcgtacaga taagcgccgt ccgctttttc gataaacagt ggagtgccgc
    17281 ccacgccagt aaaggcgcga acaggggagt tcacaccgcc agggagcagc tcgcgcgctg
    17341 cgctgtaaag attttcagac ttactcatgg agggttcctg attcgtagaa aaagtgaatg
    17401 gctgctattc tatgttattc ataacaagtt aaatactcgt caaacatcag gctgcttgta
    17461 ctggtgcaat cctgatttct tagagtataa aagttttgtg catttgaaac attacgcttt
    17521 gcaaaggatt ttcatggaac gtgcgagtaa aatgccgtca tcttatttgt atgaccaata
    17581 agtgatcatt ggatgaaaac tgatactccc tctttagaaa caccgcaggc cgcgcgcctg
    17641 cgacgcagac aactgattcg ccaacttctt gagcgcgata aaaccccgtt agccattttg
    17701 tttatggcgg cagtcgtcgg cacgcttgtc gggctggcag cggttgcttt tgacaaaggt
    17761 gtcgcctggt tgcagaacca acgtatgggg gcgctggtac atactgctga taattatccg
    17821 cttctgttaa ccgtcgcttt tctctgttcg gcggtgctgg cgatgttcgg ctactttttg
    17881 gtgcgcaaat acgcgccgga agcaggtggt tcggggatcc cggaaattga aggggcgctg
    17941 gaagatcaac gtcccgttcg ctggtggcgt gtgttgccgg tgaaattctt tggcgggctg
    18001 gggacactcg gcggaggcat ggtgctgggg cgcgaagggc caaccgtgca gatcggcggc
    18061 aatatcggtc gcatggtact cgatattttc cgcctgaaag gtgatgaagc tcgccatacg
    18121 ctgctggcaa ccggtgctgc tgcggggctg gctgcggcct ttaacgcgcc gctggcgggt
    18181 attttgttta ttatcgaaga gatgcgtccg cagtttcgct atacgttaat ttcgattaaa
    18241 gcggtattta ttggcgtcat tatgtcgacc attatgtacc ggatttttaa tcatgaagtt
    18301 gcgttgattg acgtcggtaa actttctgat gcgccgctta atacgctgtg gctttatctg
    18361 atcctcggta ttatttttgg cattttcggc cctattttta ataaatgggt gctggggatg
    18421 caggatttgc tgcaccgtgt gcacggcggc aatattacca aatgggtgct aatgggcggt
    18481 gcgattggcg gtctgtgtgg attgctgggg tttgtggcac cagcaacgtc gggcggcggt
    18541 tttaacctga ttcctatcgc taccgcaggg aatttcagca tgggaatgct ggtgtttatc
    18601 ttcgtcgcgc gggtcattac caccttactg tgcttctctt ccggcgcgcc gggcggtatt
    18661 tttgccccga tgctggcgct gggtactgtg ctgggaaccg ctttcggaat ggttgtcgtt
    18721 gagctgtttc cgcaatatca ccttgaggcg gggacttttg ctattgccgg aatgggggcg
    18781 ttactggcgg catctattcg cgcgccgtta acggggatca ttttggttct ggagatgacc
    18841 gataactacc agctcatttt gccaatgatt attaccggtc ttggcgcaac actattagcg
    18901 caatttaccg gcgggaaacc gctatattcg gagattcttg cgcgcacgct ggcaaaacag
    18961 gaagctgagc aactggcgcg aagcaaggcc gcatcagcca gcgagaatac ttgaacgaaa
    19021 taccagggta ttagataatg gcgattatta ttgggttaga atttgcccaa ttgccgatgt
    19081 cgtttggagc aaaatatgag tgatgacgta gcactgccgc tggagtttac cgacgcagca
    19141 gccaacaaag ttaaaagcct gatcgctgac gaagataacc cgaatctgaa attacgcgtg
    19201 tatatcaccg gtggcggttg cagcggcttc cagtatggtt tcacctttga tgatcaggtg
    19261 aacgaaggcg atatgaccat cgaaaaacag ggcgttggcc tggtggttga tccgatgagc
    19321 ctgcaatatc tggtcggtgg ttccgttgac tataccgaag gactggaagg ttctcgtttc
    19381 atcgtgacca acccgaacgc gaaaagcacc tgcggttgcg gttcttcctt tagcatctaa
    19441 tctgttgttc atgtgccgga taaactatcc ggcacatctt ctctcttcaa cgcccattct
    19501 catccagcgc aaacgtcggt agcttaagat gccaacgaat agccgccagc cgaatcaata
    19561 gcgtcacgac catgcccatc atactggctg tttccagtgg tacggaaaat gtgtaataag
    19621 ccgtagcgtg gacaataccg ccgataatac aggcagttgc gtagatttct gtacgtaaaa
    19681 tcatggggat ttcgcgggcc agtacatcac gaatgatccc gccgccaacg ccagtaatga
    19741 cgcccataca aaccgcgatt aacggaccgg cttccgcatt aaaggcttta ttcacgccaa
    19801 tgccgacaaa caccgccaga ccaacggcgt ccagcaccgg caacatccat tttggtaagc
    19861 gtcttggctg gcgcaccagc acgatggtca gcatgctggt taccattgca acgaccagat
    19921 cggtgggatc tttcacccaa aataccgggc cgtgatccag cgccatgtcg cgaattgtcc
    19981 cgccgcctac agcggtaacc acgcccagta ccagaacacc aaaagggtcc atacgcaatt
    20041 ttccggctaa caaaacgccg gagatggcaa ataccgctgt gccgactata tccagccaat
    20101 agacgagcat tgttcaatcc ccactgagca cctaaaaagg tcaggcgcta atctacctgt
    20161 gaaagcgcat tacagagctg ttgtgcagcg aggataatac gtgggcttgc acgtcaaacc
    20221 agtcactcgt gagaggaata acgggaattt tgagctgttc accccagtat tgtttgattt
    20281 taggaatttg gtccggtccg cctgtaatga caatcgcctg tggcgagcgt gctaacacct
    20341 gttcgcggct aacttgcggc caaggaaccc ggctgtcttt aaagatgttt tctccgccac
    20401 aaacttcgag tacctggttt tgaatcgact cttttccact ggtaaatggc ggattaatgc
    20461 cgaattgcag aaaaacacgt tttttaggtt tatcagcata ttgcgctttc aattgcgcgt
    20521 actgatccag cagggattgc gcggcttgtt cggccttatc cggttgcgga ctccaggggg
    20581 ccagttgacg taacgcattg gcaatttgtt caatgcttgt cgcatcgacc cacatcactt
    20641 ttattcccgg cgaagccagc tggtcaacct gccgctcggc attacctcca cgccaggcaa
    20701 tcaccagatc gggtttcagc gcgacaatgc gttccagatt catcccctgc caggtggaaa
    20761 cctgctcaat cttttgcgct tgtggaggat agtcggaata gctgctgacc ccaaccggcg
    20821 tgatcccggc ggcaaaggca agttcagtgt tggcgggaga aagcgtgatg acgcgcggtg
    20881 ccgcgttgag ccacagtggc gcgagaaaag acagggcgac cagcgccctg aacagtgact
    20941 tagccatgtg ccagtttctg caccagtgac tcaaccatca ggctggactg tttagcggca
    21001 acagccagga actcatcgaa gctaagatga gactgttgat cggccacgtc ggagatggcg
    21061 cgtacgacaa caaacgggac gttgaaattg tggcagacat gggcgattgc cgtcgcttcc
    21121 atctctacag caatggcctg tgggaagttg tggcggattt tcgccagacc aacagaaccg
    21181 ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag cgttaagatt cagttcggca
    21241 atgcaggcct cagcggcagc gatcagttta tcgtcagctt caaagcctgc cggacagcct
    21301 ggtaactgac cgtattcata accaaatgcc gtgacatccg cgtcgtgata acgtgcttcg
    21361 tccgagacaa cgatatcgcc cactttcaac gttggtgcca ggccaccggc agaaccggtg
    21421 ttaataatca catctggctt gcagtgttcc aacagcaaag tggcacccag cgccgcagcg
    21481 actttaccga tgcccgattt cagaagcgca acctcggttc cattcagttg gccggtatag
    21541 atttcgcaac cgccgagact gatagtttga cgattttcga ttttgtcacg cagcagcgta
    21601 acttcttctt ccattgcacc aatgatgccg attttcatag atttactcgc gataagcccg
    21661 atttgaaggc atagtttacc atgcgcttac ggggaagcgt atttctcacg cgggagagga
    21721 catggcacag attgatttcc gaaaaaaaat aaactggcat cgtcgttacc gttcaccgca
    21781 gggcgttaaa accgaacatg agatcctgcg gatcttcgag agcgatcgcg ggcgtatcat
    21841 caactctccg gcaattcgtc gtctgcaaca aaagacccag gtttttccac tggagcgcaa
    21901 tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc cagcaggtgg ggcgctacat
    21961 cgccaaagaa attctaagcc gtatgaaaga gcttaaatta ctggaagcat acggcctgga
    22021 tgaactgacc ggcccctttg aaagcattgt tgagatgtca tgcctgatgc acgatatcgg
    22081 caatccgccg tttggtcatt ttggcgaagc ggcgataaat gattggtttc gccagcgttt
    22141 gcacccggaa gatgccgaaa gccagcctct gactgacgat cgctgcagcg tggcggcact
    22201 acgtttacgg gacggggaag aaccgcttaa cgagctgcgg cgcaagattc gtcaggactt
    22261 atgtcatttt gaggggaatg cacaaggcat tcgtctggtg catacattga tgaggatgaa
    22321 tctcacctgg gcacaggttg gcggtatttt aaaatatacc cgtccggcgt ggtggcgtgg
    22381 cgaaacgcct gagacacatc actatttaat gaaaaagccg ggttattatc tttctgaaga
    22441 agcctatatt gcccggttgc gtaaagaact taatttggcg ctttacagtc gttttccatt
    22501 aacgtggatt atggaagcag ccgacgacat ctcctattgt gtggcagacc ttgaagatgc
    22561 ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg aagcgtgggg
    22621 ccagcatgag aaaggttcgc tcttttcgct ggtggttgaa aatgcctggg aaaaatcacg
    22681 ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac gggtaaacac
    22741 cctaaataaa ctggtaccct atgcggcaca acgatttatt gataatctgc ctgcgatttt
    22801 cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg atcttcttaa
    22861 gctatataaa aatgtcgctg taaaacatgt gtttagccat ccagatgtcg agcagcttga
    22921 attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt tattaagcct
    22981 gtcgttatcc gactttactg aactggtaga aaaagaacgg gtgaaacgtt tccctattga
    23041 atcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag
    23101 taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc gttgccgcct
    23161 gctgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat accgacgtct
    23221 gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt ttaccttttg
    23281 cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac agcaattttg
    23341 cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac tgagtgcact
    23401 ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg agacttcttc
    23461 agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc
    23521 agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa
    23581 tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttctc cgttccagag
    23641 ctctccgttc tgccagggtg gccagggcgg taatggtggc ggccagcaac agaaattcat
    23701 ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca ccaacaacca
    23761 cgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggccgta agttcgacgc
    23821 gaagatggtc ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc agaacccgaa
    23881 aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg attacaccgt
    23941 agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg tctctgcgct
    24001 ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg atgcagcgat
    24061 caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga tcggtatcaa
    24121 caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcaa tcccgagcaa
    24181 catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac gcggtgagct
    24241 gggtattatg gggactgagc tgaactccga actggcgaaa gcgatgaaag ttgacgccca
    24301 gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag cgggcattaa
    24361 agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg ccgcactgcg
    24421 tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac tgcgcgacgg
    24481 taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg ttgattccag
    24541 ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag atcagggcgt
    24601 ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga agaaaggtga
    24661 tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc gtaaagttct
    24721 cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca ccatctacct
    24781 gttaatgcag taatctcact caaccccttc ctgaaaacgg gaaggggttc tccttacaat
    24841 ctgtgaactt caccacaact ccatacatct tcatcatcct ttaggcattt gcacaatgcc
    24901 gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg gctggctggc
    24961 atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc atcgatacca
    25021 atatcaacgt aatggatgcc cgtgggcgaa ttatcggcag cggcgatcgt gagcgtattg
    25081 gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc gatatcgatg
    25141 acgcggtggc acgtcatctg cacggtgtgc ggcaagggat taatctaccg ttacggctgg
    25201 aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg cgtaaatatg
    25261 gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg atgcacttgt
    25321 tggcgcagga tagccgtttg cgggaggaac tggtgatgaa cctgattcag gcagaggaga
    25381 atactcccgc acttactgaa tgggcgcaac ggctggggat cgatctcaat caaccgcgag
    25441 tggtggctat tgttgaggtc gacagcggtc agcttggtgt ggacagcgca atggcggagt
    25501 tacaacaact gcaaaacgcg ctgactacgc ccgagcgtaa taatctggtg gcgattgtct
    25561 cgctaaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc tgggatgcag
    25621 aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac ggccggctgc
    25681 gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc cgatcctatc
    25741 gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt cgctgctatt
    25801 tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac tggcaggcca
    25861 acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataacggc ttgctgcgac
    25921 gaacgctggc ggcgtggttt cgtcacaatg tgcaaccgct ggcaacgtca aaggcgttgt
    25981 ttatttatcg taataccctg gagtatcggc ttaatcgtat atcggaactg accgggcttg
    26041 atttgggcaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg gatgaagagc
    26101 ggtaggttat gcgttaaggt tgaggcggcg ctgtctcatt cccctcagcc cgaaggggag
    26161 aggggaatgc ccgaattaac cgtcaattcg ggattattta cgcgttagtt tttccagatc
    26221 ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta agtcgtcgag
    26281 gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat caagctcatc
    26341 aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct gacccacacc
    26401 atcagcgacg accgttttac gctgacgcgg atatttaaac ttaacgctct tggcgaaaaa
    26461 ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt cctgccggag
    26521 gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat tgtcgtacat
    26581 agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc ctagcccgcc
    26641 cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg gcgtaaaaat
    26701 caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag ttccagcatc
    26761 gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc acgggttgtg
    26821 tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc ttcgacaggg
    26881 gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg gccgcacagc
    26941 atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg tgccagcatc
    27001 agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc ccataagtca
    27061 ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc ctggttaaac
    27121 aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt ttctgtacca
    27181 acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc aaggggggag
    27241 aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta atcgccgccc
    27301 agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga aatcaggctt
    27361 aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa tggtgctgcg
    27421 ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctgg ctcccagtaa
    27481 tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg caacaatcgg
    27541 cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg ctgcccgtgt
    27601 gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc gcgagttttc
    27661 gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc ttttgacggc
    27721 agattacctg aaacaaccac agaccccgcc ggaacgtgac cgtagtggat ttcgccggtt
    27781 tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat gacggaacct
    27841 tcttcgacaa tcaccccttc aactacttca gagcgtgcgc caatgaagca gttatcttca
    27901 atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac gccaccggaa
    27961 aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt atcaaccatg
    28021 gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac ggtgttacgg
    28081 gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc ttctttctgg
    28141 aaacgtgctt cgtcgtagtc ggcgaatttc attggcactt tgtcgaagta gcggctttct
    28201 gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc ttttttcaac
    28261 cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc accggaatcc
    28321 agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt ggttggcgtg
    28381 atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg cattgttaaa
    28441 ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc ctctgtcaac
    28501 cgctgatgca cttcctgctg caactcgtta ttaagcgcac gccggtcagc ggtggcaatt
    28561 atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg aagcgaaatt
    28621 cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc gagcgcgata
    28681 agctcaagga acgatttgcg gtcggtatgg gtcggcaaaa acgttacttc ggtttcaaca
    28741 gtaaaatggc gtaatttggc gggttggcga cggggctgcg gtggctgcca gctactttgc
    28801 gtcagtactt gctccagacc aaaccgaata acctcatgac gatctgcgga cagcgggctg
    28861 ccatcgggtt ccagcacgat aaaggtatcc atcgccatac cgtcgcgagt ggtgaaaatt
    28921 tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc ggcaaacaga
    28981 taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc ctgcgggcta
    29041 agcaatacca gtggtttgct taaatcatgc tgtaataaat ggcgggcatg ccaggccagt
    29101 tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat ttggtgcagc
    29161 gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg gtgatggcga
    29221 acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt ttcggtggca
    29281 aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc gttggtggcg
    29341 caaatgtcag ccactgtcag gcataccaga tagcgcagac gattttccgt ttgaacttct
    29401 tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc tgtcaccgac
    29461 atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga gttcagcccg
    29521 tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc gccgccgcgt
    29581 cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga cggcaggcgc
    29641 ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc aaaactctcc
    29701 agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa cagatcaaac
    29761 tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac gctatggcga
    29821 tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct caaaaacagt
    29881 tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc atggcgtaac
    29941 tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat ggtgtagaac
    30001 atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag gtcgattagc
    30061 gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc aggaagggcg
    30121 aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac gcggcgtgta
    30181 acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc actgtaattc
    30241 agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta acggctgacg
    30301 accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt taattccgcc
    30361 cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt tgcgccaaaa
    30421 tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg gctacttttg
    30481 atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg gttctgttct
    30541 tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa aatatgtttt
    30601 tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat taaattggtg
    30661 gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg cacactatga
    30721 ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt ttgcgcctga
    30781 tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag tggatgcagc
    30841 tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg gctgaatccc
    30901 gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc ctcaatcaac
    30961 tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa agtatcgata
    31021 tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg attttgcggt
    31081 tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt attcattgtg
    31141 cgccactcat aagattaaat tatcgcatta aaaaagccgg cattcgccgg cttcatctta
    31201 ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca gaatttcgca
    31261 gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc gatctttggt
    31321 ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt tgaccattgg
    31381 ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg agtcatagtg
    31441 cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat attcacgaac
    31501 gacggagaag ccttctgctt cgacaaactt ctgaatcgcc gcaccgattt cgcgcaggtt
    31561 aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga tgcggcacag
    31621 acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat cgccgtggaa
    31681 accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca gcagcttcgc
    31741 atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg atttcggata
    31801 gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt aatcattaca
    31861 gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga tcatctccag
    31921 cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg gggtcttgat
    31981 tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata tatgtaagtg
    32041 ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc caaacgtgcc
    32101 actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc gccaacaatt
    32161 attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc catttcgtat
    32221 acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg tatcggcaca
    32281 tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata accccaactt
    32341 ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca aggctggtgt
    32401 tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca tcttcggtgc
    32461 gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca acgaagctct
    32521 ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg gtactaaacg
    32581 cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct tcgtgaacca
    32641 tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca tcaaacgtct
    32701 gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca agaaagaagc
    32761 gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta tcaaagacat
    32821 gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca ttgctatcaa
    32881 agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact ctgatccgga
    32941 cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga ccctgtacct
    33001 gggcgctgta gctgcaaccg tacgtgaagg ccgttctcag gatctggctt cccaggcgga
    33061 agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat agttcgagtt
    33121 gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg accgggatga
    33181 gcgagcgaag ataacgcatc tgcggcgcga aatatgaagg gggagagccc ttatagacca
    33241 ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt ttatatctgt
    33301 gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga aattaccgca
    33361 tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg caaaaaagca
    33421 ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa gtccggtgct
    33481 attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa aaccaaaatc
    33541 gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt tgcaaaagac
    33601 gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg caaaatcact
    33661 gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt agcgaaaatt
    33721 ggtgaaaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct gggttcttat
    33781 cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga agagctggtt
    33841 aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc agaagacgta
    33901 tccgctgaag tggtagaaaa agaataccag gtacagctgg atatagcgat gcagtctggt
    33961 aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt caccggcgaa
    34021 gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg tcagctgctg
    34081 aaagagcata acgctgaagt gactggcttc atccgcttcg aagtgggtga aggcatcgag
    34141 aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc ttaattatca
    34201 aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc agctaaccct
    34261 tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat ttcaatcgtt
    34321 gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa cgcattctgc
    34381 ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat gcaagcatac
    34441 tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag gttggtgtgg
    34501 tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt atgaaccgcg
    34561 ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca atgcgtgatg
    34621 cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg aatggcgtgt
    34681 gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt gtggtgatcc
    34741 tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc ctgcgtggta
    34801 tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg tttaccgctg
    34861 atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc gaagtgctgg
    34921 aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac cataaattac
    34981 cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta atgggtgaaa
    35041 aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt aagattccgc
    35101 gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg cagtttggtc
    35161 acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga gcactgctcg
    35221 gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta atatgtttaa
    35281 tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac tgagacaagt
    35341 tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta cgcatggaca
    35401 aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt gcttctccca
    35461 gcctgctgga tggcattgtc gtagaatatt acggcacgcc gacgccgctg cgtcagctgg
    35521 caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat cgttcaatgt
    35581 ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg aactctgcgg
    35641 gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa gatctgacca
    35701 aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg cgtcgtgacg
    35761 cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac gacgatcgcc
    35821 gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt gaagcggcgc
    35881 tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa aaacgccgct
    35941 cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct caactctgga
    36001 tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg cagcacgctg
    36061 gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc aggcaaaaat
    36121 gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt aatggacgat
    36181 gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg caccgaagtc
    36241 ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga tcaggtgatg
    36301 gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg cgcgggtaaa
    36361 accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt tatggacgcc
    36421 gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc catttttcag
    36481 agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca aaatggcgtg
    36541 gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt gcgcgatttg
    36601 gcaacaatga cgccggatca agcctgccgt catccgaact ggtcgatggg gcgtaaaatt
    36661 tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc gcgttggcta
    36721 tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt gattcactca
    36781 atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga tatgcgtacg
    36841 ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa gccgctcgat
    36901 ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta tccatgcctg
    36961 aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt gaatgccgca
    37021 aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga tatcgctgcg
    37081 ttgaatttat cggtactgga aaaaatggat atgcgcgaac cacaatgtgt ggacgatgtg
    37141 ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg tctcgcaagc
    37201 tgaggataat ccggctacag agagtctcgc tatttgttag cgtagggctt cagtgatata
    37261 gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc gtggttttgc
    37321 acggcttttt ttgtataggc ttcagtattc ctgagtaccg taaaccctgt cagggaataa
    37381 aaaacgcgtg atgttgtctg ctactcaact acttagcgaa aaattgccag cgcatggctg
    37441 ccgtcatgtt gcgatcatta tggacggcaa tggtcgctgg gcaaaaaagc aagggaagat
    37501 tcgtgccttt gggcataaag ccggggcaaa atccgtccgc cgggctgtct cttttgcggc
    37561 caacaacggt attgaggcgt taacgctgta tgcctttagt agtgaaaact ggaaccgacc
    37621 agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg gcgctcgata gcgaagtaaa
    37681 aagtctgcac cgacataacg tgcgtctgcg tattattggc gataccagtc gctttaactc
    37741 gcgtttgcaa gaatgtattc gtaaatctga agcgctaaca gccgggaata ccggtctgac
    37801 gctgaatatt gcggcgaact acggtggacg ttgggatata gtccagggag tcaggcaact
    37861 ggctgaaaag gtgcagcaag gaaacctgca accagatcag atagatgaag agatgctaaa
    37921 ccagcatgtc tgtatgcatg aactggcccc tgtagattta gtaattagga ctggggggga
    37981 gcatcgcatt agtaactttt tgctttggca aattgcctat gccgaacttt actttataga
    38041 tgttctctgg cccgatttcg atgaacaaga ctttgaaggg gcgttaaatg cctttgctaa
    38101 tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa acagcctgat ggggggcgct
    38161 tttgctgaag tatcgcctga tatctgcttt tgtgttaata cccgtcgtca tcgcggcgtt
    38221 gtttctgttg ccgccggtgg ggttcgccat tgtaacgctg gtggtctgca tgctggcagc
    38281 gtgggaatgg ggacagctta gcggttttac cactcgttcg cagcgagtat ggttggcggt
    38341 gttatgcggg ttattgttgg cgctgatgct ttttctgttg ccggaatatc accgaaatat
    38401 tcatcaaccg ctggttgaaa tctcactttg ggcttcgctg ggttggtgga ttgtctcgct
    38461 attgctggtg ctgttttacc caggttccgc agcaatctgg cgtaactcta aaacattgcg
    38521 ccttattttt ggcgtgctaa ccattgttcc cttcttctgg ggcatgctgg cgttacgggc
    38581 ctggcactat gacgagaatc attacagtgg cgcaatatgg ctgctctatg tcatgatcct
    38641 ggtatggggc gctgactccg gcgcatatat gtttggcaaa ttgtttggta aacataagct
    38701 ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt ataggtggac tcgctactgc
    38761 agcggtaatc tcatggggtt atggcatgtg ggcgaatctc gacgtcgctc ccgtcacctt
    38821 actcatttgc tctattgtcg cagcgttagc ctcagtgctc ggcgatctga ccgagagtat
    38881 gtttaagcgt gaagcaggaa ttaaggacag cggtcattta attccaggac acggtggtat
    38941 tttagatcgt attgatagcc tgacggctgc ggtaccggtc tttgcttgct tgttgttact
    39001 ggtattcagg acgctttaac ggaaggtaat atgctgagtt ttctctggga tttggcttcg
    39061 ttcatcgttg cactgggtgt acttatcact gtgcatgaat ttggtcattt ctgggttgcc
    39121 cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt ttggtaaggc gctctggcgg
    39181 cgaactgata agctcggcac cgaatatgtt atcgccctga tcccgctggg cggttatgtc
    39241 aaaatgctgg atgagcgcgc agaaccggtc gttccggaac tccgccacca tgccttcaat
    39301 aataaatctg ttggccaacg agcggcgatt attgccgcag gtccggttgc aaacttcatt
    39361 tttgctatct ttgcctactg gctggttttt attattggtg tgcctggcgt acgtccggtg
    39421 gttggtgaaa tagcagccaa ttcgatagct gcggaagcac aaattgcacc aggtacggaa
    39481 ctaaaagccg tagatggtat cgaaacgcct gattgggatg ccgtgcgttt gcagttggtc
    39541 gataaaattg gcgatgaaag caccaccatt acagtagcgc catttggcag cgaccaacgg
    39601 cgggatgtaa agctcgattt acgtcactgg gcgtttgagc ctgataaaga agatccggta
    39661 tcttcgctgg ggattcgtcc tcgtgggctg caaattgaac ctgtactgga aaatgtgcag
    39721 ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg acaggatcgt taaagtcgat
    39781 ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg tccgggataa cccggggaaa
    39841 tccttagcgt tagaaatcga aaggcaggga agtcccttgt ctttgacatt aatcccggag
    39901 agtaaaccgg gtaatggtaa agcgattggt tttgtcggta ttgagccgaa agtcattcct
    39961 ttgccagatg agtataaagt tgtacgccag tatgggccgt tcaacgccat cgtcgaagcc
    40021 acggacaaaa cgtggcagct gatgaagctg acggtcagta tgctgggaaa attgatcacc
    40081 ggtgatgtga aactgaacaa cctcagtggg ccgatctcta tcgccaaggg ggctgggatg
    40141 acagcggaac tcggggtagt ttattacctg ccgtttcttg cgcttattag cgtgaactta
    40201 gggataatta acctgtttcc gttgcccgta cttgacgggg cgcatctgct gttccttgcg
    40261 atcgaaaaga tcaagggcgg accggtatcc gagcgggttc aagacttttg ttatcgcatt
    40321 ggctcgattc tgctggtgct gttaatgggg cttgcacttt tcaatgattt ctctcggtta
    40381 tgagagttag ttaggaagaa cgcataataa cgatggcgat gaaaaagttg ctcatagcgt
    40441 cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg gttcgtagtg aaagatattc
    40501 atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct cctcagtatg ccggtgcgta
    40561 caggcgacac ggttaatgat gaagatatca gtaataccat tcgcgctctg tttgctaccg
    40621 gcaactttga ggatgttcgc gtccttcgtg atggtgatac ccttctggtt caggtaaaag
    40681 aacgtccgac cattgccagc attactttct ccggtaacaa atcggtgaaa gatgacatgc
    40741 tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga atccctcgat cgcaccacca
    40801 ttgccgatat cgagaaaggt ctggaagact tctactacag cgtcggtaaa tatagcgcca
    40861 gcgtaaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt tgacctaaaa ctggtgttcc
    40921 aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt tggtaaccat gctttcacca
    40981 ccgacgaact gatctctcat ttccaactgc gtgacgaagt gccgtggtgg aacgtggtag
    41041 gcgatcgtaa ataccagaaa cagaaactgg cgggcgacct tgaaaccctg cgcagctact
    41101 atctggatcg cggttatgcc cgtttcaaca tcgactctac ccaggtcagt ctgacgccag
    41161 ataaaaaagg tatttacgtc acggtgaaca ttaccgaagg cgatcagtac aagctttctg
    41221 gcgttgaagt gagcggcaac cttgccgggc actccgctga aattgagcag ctgactaaga
    41281 tcgagccggg cgagctgtat aacggcacca aagtgaccaa gatggaagat gacatcaaaa
    41341 agcttctcgg tcgctatggt tatgcctatc cgcgcgtaca gtcgatgcct gaaattaacg
    41401 atgccgacaa aaccgttaaa ttacgcgtga acgttgatgc gggtaaccgt ttctacgtgc
    41461 gtaagatccg ttttgaaggt aacgatacct cgaaagatgc cgtcctgcgt cgcgaaatgc
    41521 gtcagatgga aggtgcatgg ctgggaagcg atctggtcga tcagggtaag gagcgtctga
    41581 atcgtctggg cttctttgaa actgtcgata ccgataccca acgtgttccg ggtagcccgg
    41641 accaagtaga tgtagtctac aaggtgaaag aacgtaacac cggtagcttc aacttcggga
    41701 ttggttacgg tactgaaagt ggcgtgagct tccaggctgg cgtgcaacag gataactggt
    41761 taggtacagg ttatgctgtt ggtatcaacg ggaccaaaaa cgattaccag acctatgctg
    41821 aattgtcggt aaccaacccg tacttcactg tagatggtgt aagcctcggt ggtcgtctct
    41881 tctataatga cttccaggca gatgacgccg acctgtccga ctataccaac aagagttatg
    41941 gtacagacgt gacgttgggc ttcccgatca acgaatataa ctcgctgcgt gcaggtctgg
    42001 gttatgtaca taactccctg tccaacatgc agcctcaggt tgcgatgtgg cgttatctgt
    42061 actctatggg tgaacatccg agcacctctg atcaggataa cagcttcaaa acggacgact
    42121 tcacgttcaa ctatggttgg acctataaca agcttgaccg tggttacttc ccgacagatg
    42181 gttcacgtgt caacctgacc ggtaaagtga ccattcctgg atcggataac gaatactaca
    42241 aagtgacgtt agacacggcg acttatgtgc cgatcgatga cgatcacaaa tgggttgttc
    42301 tggggcgtac ccgctggggt tatggtgatg gtttaggcgg caaagagatg ccgttctacg
    42361 agaacttcta tgccggtggt tccagcaccg tccgtggctt ccagtccaat accattggtc
    42421 cgaaagcagt ttacttcccg catcaggcca gtaattatga tccggactat gattacgaat
    42481 gtgcgactca ggacggcgcg aaagacctgt gtaaatcgga tgatgctgta ggcggtaacg
    42541 ccatggcggt tgccagcctc gagttcatca ccccgacgcc gtttattagc gataagtatg
    42601 ctaactcggt tcgtacttcc ttcttctggg atatgggtac cgtttgggat acaaactggg
    42661 attccagcca atattctgga tatccggact atagtgatcc aagcaatatc cgtatgtctg
    42721 cgggtatcgc attacaatgg atgtccccat tggggccgtt ggtgttctcc tacgcccagc
    42781 cgttcaaaaa gtacgatgga gacaaggcag aacagttcca gtttaacatc ggtaaaacct
    42841 ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc gatgacttta ggcgatcaat
    42901 ataagatcgc cgggccacgc aaagaactgc accctccggt gcaaatggga tggtaaggag
    42961 tttattgtga aaaagtggtt attagcggca ggtctcggtt tagcactggc aacttctgct
    43021 caggcggctg acaaaattgc aatcgtcaat atgggcagcc tgttccagca ggtagcgcag
    43081 aaaaccggtg tttctaacac gctggaaaat gagttcaaag gccgtgccag cgaactgcag
    43141 cgtatggaaa ccgatctgca ggctaaaatg aaaaagctgc agtccatgaa agcgggcagc
    43201 gatcgcacta agctggaaaa agacgtgatg gctcagcgcc agacttttgc tcagaaagcg
    43261 caggcttttg agcaggatcg cgcacgtcgt tccaacgaag aacgcggcaa actggttact
    43321 cgtatccaga ctgctgtgaa atccgttgcc aacagccagg atatcgatct ggttgttgat
    43381 gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca tcactgccga cgtactgaaa
    43441 caggttaaat aagtaatgcc ttcaattcga ctggctgatt tagcgcagca gttggatgca
    43501 gaactacacg gtgatggcga tatcgtcatc accggcgttg cgtccatgca atctgcacaa
    43561 acaggtcaca ttacgttcat ggttaaccca aaataccgtg agcatttagg cttgtgccag
    43621 gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg cgaaaagtgc cgcgctggta
    43681 gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa ttttagatac cacgccgcag
    43741 cccgcgcaga acattgcacc cagtgcggtg atcgacgcga cggcgaagct ggggaacaat
    43801 gtatcgattg gcgctaacgc ggtgattgaa tccggcgttg aactgggcga taacgtgatt
    43861 atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg gtgcaggttc gcgtctctgg
    43921 gcgaacgtaa ccatttacca tgagatccag atcggtcaga attgcctgat ccagtccgga
    43981 acagtggtag gcgcagacgg ctttggttat gccaacgatc gtggtaactg ggttaagatc
    44041 ccacagattg gtcgcgtaat tattggcgat cgcgtggaga tcggtgcctg cactaccatc
    44101 gatcgcggcg cactggatga cactgttatt ggcaatggcg tgatcattga taaccagtgc
    44161 cagattgcac ataacgtcgt gattggcgac aatacggcgg ttgccggtgg cgtcattatg
    44221 gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg gagccagcgt aatcaacggg
    44281 catatggaaa tatgcgacaa agtgacggtt acgggcatgg gtatggtgat gcgtcccatc
    44341 actgaaccag gcgtctattc ctcaggcatt ccgctgcaac ccaacaaagt ctggcgcaaa
    44401 accgctgcac tggtgatgaa cattgatgac atgagcaagc gtctgaaatc gcttgagcgc
    44461 aaggttaatc aacaagacta acgttccatc ttttgttcgc caaactttac ggcctgtctc
    44521 attcttacga ttgcggcagg ccgtgttatt attgtcgttt cttatatttt gacaggaaga
    44581 gtatcttgac tactaacact catactctgc agattgaaga gattttagaa cttctgccgc
    44641 accgtttccc gttcttactg gtggatcgcg tgctggattt tgaagaaggt cgttttctgc
    44701 gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca gggccatttc cctggaaaac
    44761 cgattttccc gggtgtgctg attctggaag caatggcaca ggcaacaggt attctggcgt
    44821 ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta cttcgctggt attgacgaag
    44881 cgcgcttcaa gcgcccggtc gtgcctggcg atcaaatgat catggaagtc actttcgaaa
    44941 aaacgcgccg cggcctgacc cgttttaaag gggttgctct ggtcgatggt aaagtagttt
    45001 gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg atacgtgatt gataaatccg
    45061 cctttgtgca tccaaccgcc attgtggaag agggcgcgtc aattggcgcg aacgcacaca
    45121 ttggtccttt ttgtatcgtt ggaccccatg tcggaattgg tgagggtacc gtactgaaat
    45181 ctcacgttgt cgtgaatggt catactaaaa ttggccgcga taatgagatt tatcagttcg
    45241 cctccatcgg cgaagttaac caggatctga aatatgctgg cgaaccgacc cgtgtggaaa
    45301 tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca tcgtggcaca gtccagggcg
    45361 gtggattgac gaaggtgggc agcgacaact tactgatgat caacgcgcac attgcgcacg
    45421 attgtacggt aggtaaccgc tgtattctcg ccaacaacgc aacgctggcg ggtcacgtat
    45481 cggttgacga cttcgcgatc atcggcggca tgaccgcagt ccatcagttc tgcatcattg
    45541 gtgcgcacgt gatggttggc ggctgctccg gtgtggcgca ggacgtccct ccttatgtca
    45601 ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat cgaagggctg aagcgccgcg
    45661 gattcagccg tgaggcgatt accgctatcc gcaatgcgta taagctgatt tatcgtagcg
    45721 gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact ggcggaaaca tatccggaag
    45781 tgaaagcctt taccgatttc ttttcacgct caacgcgcgg tctgattcgt taatgactga
    45841 acagcgtcca ttaacgattg ccctggtcgc cggagaaacc tccggcgata tcctgggggc
    45901 cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc cgctttgttg gtgttgccgg
    45961 gccacgaatg caggctgaag gctgcgaagc ctggtacgaa atggaagaac tggcggtgat
    46021 gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg catattcgtg ccgatctgac
    46081 aaagcgtttt ggcgaactga agccagatgt ttttgttggt attgatgcgc ctgacttcaa
    46141 tattactctt gaaggtaacc tcaaaaagca gggtatcaaa accattcatt acgtcagtcc
    46201 gtcagtctgg gcgtggcgac agaaacgtgt tttcaaaata ggcagagcca ccgatctggt
    46261 gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa tacaacgtac cgtgccgctt
    46321 tatcggtcat accatggctg atgccatgcc attagatcca gataaaaatg ccgcccgtga
    46381 tgtgctgggg atccctcacg atgcccactg cctggcgttg ctaccgggga gccgtggtgc
    46441 agaagttgaa atgcttagtg ccgatttcct gaaaacggcc cagcttttgc gccagacata
    46501 tccggatctc gaaatcgtgg tgccactggt gaatgccaaa cgccgcgagc agtttgaacg
    46561 catcaaagct gaagtcgcgc gagacttttc agttcatttg ctggatggga tgggccgtga
    46621 ggcgatggtc gccagcgatg cggcgctact ggcgtcgggt acggcagccc tggagtgtat
    46681 gctggcgaaa tgcccgatgg tggtgggata tcgcatgaag ccttttacct tctggttggc
    46741 gaagcggctg gtgaaaactg attatgtctc gctgccaaat ctgctggcgg gcagagagtt
    46801 agtcaaagag ttattgcagg aagagtgtga gccgcaaaaa ctggctgcgg cgctgttacc
    46861 gctgttggcg aacgggaaaa tcagccacgc gatgcacgat accttccgtg aactgcatca
    46921 gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc gttctggagt tagcacaatg
    46981 atcgaatttg tttatccgca cacgcagctg gttgcgggtg tggatgaagt cggacgcggg
    47041 ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg acccggcgcg cccgattgcc
    47101 gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc tggcgctctg tgaagagatc
    47161 aaagagaaag cgttgagctg gagtctgggc cgcgcggaac cccacgaaat cgacgagctg
    47221 aacattcttc atgcgaccat gctggcgatg cagcgtgccg tcgctgggct gcatattgcg
    47281 ccggaatatg tgttgattga tggtaaccgc tgcccgaaat taccgatgcc tgcgatggct
    47341 gtggtgaaag gcgatagccg cgtaccggaa atcagtgccg cgtctatcct ggcgaaagtg
    47401 acgcgtgacg ccgaaatggc ggcgctggat attgttttcc cgcaatatgg ttttgcccaa
    47461 cacaaagggt acccaaccgc ttttcatctg gaaaaactgg ctgaacacgg cgcgaccgaa
    47521 caccatcggc gcagctttgg gcctgtcaaa cgcgcactgg gacttgcgtc ctgattcttg
    47581 tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac cacgtttcgt acacctgcgg
    47641 gtgcacagcg actactcgat gatcgatggc ctggccaaaa ctgcaccgtt ggtaaaaaag
    47701 gcggcggcgt tgggtatgcc agcactggcg atcaccgatt tcaccaacct ttgtggtctg
    47761 gtgaagttct acggagcggg acatggcgca gggattaagc ctatcgtcgg ggcagatttt
    47821 aacgtccagt gcgacctgct gggtgatgag ttaacccacc tgacggtact ggcggcgaac
    47881 aataccggct atcagaatct gacgttgctg atctcaaaag cgtatcagcg cgggtacggt
    47941 gccgctgggc cgatcatcga tcgcgactgg cttatcgaat taaatgaagg gttgatcctt
    48001 ctttccggcg ggcgcatggg cgacgtcgga cgcagtcttt tgcgtggtaa cagcgcgctg
    48061 gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg atcgctattt tctcgagctg
    48121 atccgcaccg gcaggccgga tgaagaaagc tatctgcacg cggcggtgga actggcggaa
    48181 gcgcgcggtt tgcccgtcgt ggcgactaac gatgtgcgct ttatcgacag cagcgacttt
    48241 gacgcacacg aaatccgcgt cgcgatccac gacggcttta ccctcgacga tcctaaacgc
    48301 ccgcgtaact attcgccgca gcaatatatg cgtagcgaag aggagatgtg tgagctgttt
    48361 gccgacatcc ccgaagccct tgccaacacc gttgagatcg ccaaacgctg taacgtaact
    48421 gtgcgtcttg gtgaatactt cctgccgcag ttcccgaccg gggacatgag caccgaagat
    48481 tatctggtca agcgtgcaaa agagggcctg gaagagcgtc tggccttttt attccctgac
    48541 gaggaagaac gtgttaagcg ccgcccggaa tatgacgaac gtctggagac tgaacttcag
    48601 gttatcaacc agatgggctt cccgggctac ttcctcatcg ttatggaatt tatccagtgg
    48661 tcgaaagata acggcgtacc ggtagggcca ggccgtggct ccggtgcggg ttcactggtg
    48721 gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat ttgacctgct gttcgaacgt
    48781 ttccttaacc cggaacgtgt ctccatgcct gacttcgacg ttgacttctg tatggagaaa
    48841 cgcgatcagg ttatcgagca cgtagcggac atgtacggtc gtgatgcggt atcgcagatc
    48901 atcaccttcg gtacaatggc ggcgaaagcg gtgatccgcg acgtaggccg cgtgctgggg
    48961 catccgtacg gctttgtcga tcgtatctcg aaactgatcc cgcccgatcc ggggatgacg
    49021 ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa tctacgaagc ggatgaagaa
    49081 gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg tcacccgtaa cgccggtaag
    49141 cacgccggtg gggtggttat cgcgccgacc aaaattaccg attttgcgcc gctttactgc
    49201 gatgaagagg gcaaacatcc ggtcacccag tttgataaaa gcgacgttga atacgccgga
    49261 ctggtgaagt tcgacttcct tggtttgcgt acgctcacca tcatcaactg ggcgctggag
    49321 atgatcaaca agcggcgggc gaagaatggc gagccgccgc tggatatcgc cgcgatcccg
    49381 ctggatgaca agaaaagctt cgacatgctg caacgctcgg aaaccacggc ggtattccag
    49441 cttgaatcgc gcggcatgaa ggatctgatc aagcgtctgc aacctgactg cttcgaagat
    49501 atgatcgcac tggtggcgct gttccgccca ggtccgttgc aatcagggat ggtggataac
    49561 tttatcgacc gtaaacatgg tcgcgaagaa atctcctatc cggacgtaca gtggcagcat
    49621 gaaagcctga aaccggtact ggagccaacc tacggcatca tcctgtatca ggaacaggtc
    49681 atgcagattg cgcaggtgct ttctggttat accctcggtg gcgcggatat gctgcgtcgt
    49741 gcgatgggta agaaaaagcc ggaagagatg gctaagcagc gttctgtatt tgctgaaggt
    49801 gcagaaaaga acggagtcaa cgccgaactg gcgatgaaaa tcttcgacct ggtggagaaa
    49861 ttcgcgggtt acggatttaa caaatcgcac tctgcggcct atgctttggt gtcatatcaa
    49921 acgttatggc tgaaagcgca ctatccggcg gagtttatgg cggcggtaat gaccgccgat
    49981 atggacaaca ccgagaaggt ggtgggcctg gtggatgagt gctggcggat ggggctgaaa
    50041 atcctgccac cagatataaa ctccggtctt taccatttcc acgtcaacga cgacggcgaa
    50101 atcgtgtatg gtattggcgc catcaaaggg gtaggtgaag gtccgattga ggccatcatc
    50161 gaagcccgta ataaaggcgg ctacttccgc gaactgtttg atctctgcgc ccgtaccgac
    50221 accaaaaagt taaaccggcg ggtgctggaa aaactgatca tgtccggggc gtttgaccgt
    50281 cttgggccac accgcgcggc gctgatgaac tcgctgggtg atgcgttaaa agcggcagat
    50341 caacacgcga aagcggaagc tatcggtcag gccgatatgt tcggcgtgct ggccgaagag
    50401 ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt ggccggagca ggtggtgtta
    50461 gatggggaac gtgaaacgtt aggtctgtac cttacgggac accctatcaa ccagtattta
    50521 aaagagattg agcgttatgt cggaggcgta aggctgaaag acatgcaccc gacagaacgt
    50581 ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc gggttatggt caccaagcgc
    50641 ggcaatcgta tcggtatctg cacgctggat gaccgttccg ggcggctgga agtgatgttg
    50701 tttactgacg ccctggataa ataccagcaa ttgctggaaa aagaccgcat acttatcgtc
    50761 agcggacagg tcagctttga tgacttcagc ggtgggctta aaatgaccgc tcgcgaagtg
    50821 atggatattg acgaagcccg ggaaaaatat gctagcgggc ttgctatctc gctgacggac
    50881 aggcaaattg atgaccagct tttaaaccga ctccgtcagt ctctggaacc ccaccgctct
    50941 gggacaattc cagtacatct ctactatcag agggcggatg cacgcgcgcg gttgcgtttt
    51001 ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa acgatctccg tggcctcatt
    51061 ggttcggagc aggtggaact ggagtttgac taatacagga atactatgag tctgaatttc
    51121 cttgattttg aacagccgat tgcagagctg gaagcgaaaa tcgattctct gactgcggtt
    51181 agccgtcagg atgagaaact ggatattaac atcgatgaag aagtgcatcg tctgcgtgaa
    51241 aaaagcgtag aactgacacg taaaatcttc gccgatctcg gtgcatggca gattgcgcaa
    51301 ctggcacgcc atccacagcg tccttatacc ctggattacg ttcgcctggc atttgatgaa
    51361 tttgacgaac tggctggcga ccgcgcgtat gcagacgata aagctatcgt cggtggtatc
    51421 gcccgtcttg acggtcgtcc ggtgatgatc attggtcatc aaaaaggtcg tgaaaccaaa
    51481 gaaaaaattc gccgtaactt tggtatgcca gcgccagaag gttaccgcaa agcactgcgt
    51541 ctgatgcaaa tggctgaacg ctttaagatg cctatcatca cctttatcga caccccgggg
    51601 gcttatcctg gcgtgggcgc agaagagcgt ggtcagtctg aagccattgc acgcaacctg
    51661 cgtgaaatgt ctcgcctcgg cgtaccggta gtttgtacgg ttatcggtga aggtggttct
    51721 ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata tgctgcaata cagcacctat
    51781 tccgttatct cgccggaagg ttgtgcgtcc attctgtgga agagcgccga caaagcgccg
    51841 ctggcggctg aagcgatggg tatcatcgct ccgcgtctga aagagctgaa actgatcgac
    51901 tccatcatcc cggaaccgct gggcggcgct caccgtaacc cggaagcgat ggcggcatcg
    51961 ttgaaagcgc aactgctggc ggatctggcc gatctcgatg tgttaagcac tgaagattta
    52021 aaaaatcgtc gttatcagcg cctgatgagc tacggttacg cgtaattcgc aaaagttctg
    52081 aaaaagggtc acttcggtgg ccctttttta tcgccacggt ttgagcaggc tatgattaag
    52141 gaaggatttt ccaggaggaa cacatgaaca tcattgccat tatgggaccg catggcgtct
    52201 tttataaaga tgagcccatc aaagaactgg agtcggcgct ggtggcgcaa ggctttcaga
    52261 ttatctggcc acaaaacagt gttgatttgc tgaaatttat cgaacataac cctcgaattt
    52321 gcggcgtgat ttttgactgg gatgagtaca gtctcgattt atgcagcgat atcaatcaac
    52381 ttaatgaata tctcccgctt tatgccttca tcaacaccca ctcgacgatg gatgtcagcg
    52441 tgcaggatat gcggatggcg ctctggtttt ttgaatatgc gctggggcag gcggaagata
    52501 tcgccattcg tatgcgtcag tacaccaacg aatatcttga taacattacg ccgccgttca
    52561 cgaaagcctt gtttacctac gttaaagagc ggaagtacac cttttgtacg ccggggcata
    52621 tgggcggcac cgcatatcaa aaaagcccgg ttggctgcct gttttatgat tttttcggcg
    52681 ggaataccct caaggctgat gtctctattt cggtcaccga gcttggttcg ttgctcgacc
    52741 acaccgggcc acacctggaa gcggaagagt acatcgcgcg gacttttggc gcggaacaga
    52801 gttatatcgt taccaacgga acatcgacgt cgaacaaaat tgtgggtatg tacgccgcgc
    52861 catccggcag tacgctgttg atcgaccgca attgtcataa atcgctggcg catctgttga
    52921 tgatgaacga tgtagtgcca gtctggctga aaccgacgcg taatgcgttg gggattcttg
    52981 gtgggatccc gcaccgtgaa tttactcgcg acagcatcga agagaaagtc gctgccacca
    53041 cgcaggcaca atggccggtt catgcggtga ttaccaactc cacctatgat ggcttgctct
    53101 acaacaccga ctggatcaaa cagacgctgg atgtcccgtc gatccacttc gattccgcct
    53161 gggtgccgta cacccatttt catccgatct accaggggaa aagtgatctt cgaaacgcaa
    53221 tcgacccaca aaatgctggc ggcgttatcg caggcttcgc tgatccacat taaaggcgag
    53281 tatgacgaag aggcgtttaa cgaagccttt atgatgcata ccaccacctc gcccagttat
    53341 cccattgttg cttcggttga gacggcggcg gcgacgctgc gtggtaatcc gggcaaacgg
    53401 ctgattaacc gttcggtgga gcgggcgctg cattttcgca aagaggtcca gcggttgcgg
    53461 gaagagtctg acggttggtt tttcgatatc tggcaaccgc cgcaggtgga tgaagccgaa
    53521 tgctggcccg ttgcgccagg cgaacagtgg cacggcttta acgatgcgga tgccgatcat
    53581 atgtttctcg atccggttaa agtcactatt ttgataccgg ggatggacga acagggcaat
    53641 atgagcgagg aggggatcct ggcggcgctg gtggcaaaat tcctcgacga acgtgggatc
    53701 gtagtagaga aaaccggccc ttataacctg ctgtttctct ttagtattgg tatcgataaa
    53761 accaaagcaa tgggattatt gcgtgggttg acggaattta aacgctctta cgatctcaac
    53821 ctgcggatca aaaatatgct acccgatctc tatgctgaag atcccgattt ctaccgcaat
    53881 atgcgtattc aggatctggc gcaagggatc cataagctga ttcgtaaaca cgatcttccc
    53941 ggtttgatgt tgtgggcatt cgatactttg ccggaaatta tcatgacgcc acatcaggca
    54001 tggcagagac agattaaagg tgaagtagaa accattgcgc tggaacaact ggtcggtaga
    54061 gtatcggcaa atatgatcct gccttatcca ccgggcgtac cgctgttgat gccaggagaa
    54121 atgctgacca aagagagccg cacggtactc gattttctac tgatgctttg ttccgtaggg
    54181 caacattacc ccggttttga aacggatatt cacggcgcga aacaggacga agacggcgtt
    54241 taccgcgtac gagtcctaaa aatggcggga taacttgcca gagcggctta cgggcgagta
    54301 acgtgctgtt aacaaataaa ggagacgtta tgctgggttt aaaacaggtt caccatattg
    54361 cgattattgc gacggattat gcggtgagca aagctttcta ctgcgatatt cttggtttca
    54421 cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg gaaaggggat ttggcgctta
    54481 atgggcaata tgtgattgag cttttctctt ttccgttccc gccggagcgc cccagccgac
    54541 cggaagcttg cggcctgcgt catctggctt ttagcgttga tgacatcgat gcggcagtgg
    54601 cgcaccttga aagccataac gtgaagtgtg aagccatccg tgtcgatcca tacacgcaaa
    54661 aacgcttcac cttctttaac gatccggacg ggctgccgtt ggaactgtat gagcagtaag
    54721 gcttgtcatc gccgcatttg cccggtaacg tgccgggcat tgctactgta aaatcgcacc
    54781 atcatgacac tcacgctcaa tagacaactt ctcacctcac gccagatttt ggtcgccttt
    54841 agcggcgggc ttgactccac cgttctgctg catcagttgg tgcagtggcg gacggaaaat
    54901 ccgggcgtca ctctgcgcgc tattcatgtg catcacggtt taagtgccaa tgccgatgcc
    54961 tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg tgccgctggt ggtcgaacgc
    55021 gtacaacttg cgcaagaggg gctggggatt gaggcccagg cgcggcaggc gcgttatcag
    55081 gcatttgccc gtaccctatt gcccggcgaa gtgctggtca ccgcgcaaca tctcgacgat
    55141 caatgtgaaa cctttctgct ggcgctaaaa cgcggtagcg gccctgccgg gctttcggct
    55201 atggcggaag tttcggagtt tgccggaaca cggcttatcc gcccgttgct tgcccgcacg
    55261 cggggggaac tggcgcagtg ggcattggcg catggtttac gctggattga agacgaaagt
    55321 aatcaggacg actcatacga tcgtaacttt ctgcgcctgc gcgtagtgcc gttattgcag
    55381 cagcgttggc cgcattttgc ggaagcaacg gcccgcagcg ccgcactttg tgctgaacaa
    55441 gagagtctgt tggatgaact gctggcggat gatttagcac actgccaaac gtcgcagggg
    55501 acgctgcaga ttgcgccaat gctggcgatg agtgatgccc gccgcgcggc gattatccgt
    55561 cgctggctgg cagggcagaa tgcaccaatg ccttcccgcg acgcgctggt gcggatctgg
    55621 caggaagtgg cgctggcgcg ggaagatgcc tccccctgtt tacgcttggg cgcgtttgaa
    55681 atccgccgct atcagtcgca actgtggtgg attaaatccg tcaccgggca aagtgaaacc
    55741 attgtgccgt ggcagacgtg gctacaaccg ctggaattac cggcggggct gggaagcgta
    55801 cagcttactg cgggaggcga tattcgccct ccgcgtgcag acgaagcggt cagcgtgcgt
    55861 ttcaaatcgg caggattgct gcatattgtc gggcgcaacg gcggacgtaa gctgaagaaa
    55921 atctggcaag agttaggcgt gccgccgtgg ctacgtgaca ccacgccact gctgttttat
    55981 ggcgaaacgc tgattgcggc ggcaggggta tttgtgacgc aagaaggtgt ggctgaaggt
    56041 gagaatggcg taagttttgt ctggcagaaa acgcttagtt aagtgaaagc cggataagac
    56101 gcatcaaacg tcgcatccgg cgaaagtcaa tcaggactcg ctcaccacca ccgtaccgat
    56161 ttccgggtgg ctaaagctgg taattttatc cagacgtagc tcacgggttt cgccagcggc
    56221 ctcgacgacc agatactcca catttttgcg ggagactaaa tcgctggctt ttgcctgcaa
    56281 tttttcgcca tctttcagct caagtgtcag cattaaatga tgctggcagg cgagctcaag
    56341 attatcgtaa tcatcacaat tgattggttg atacgtatca ttcattaaca taatcgctca
    56401 ccagtaagtt tgccgcagcg tatgctgctt tttccctgac agcctcagaa agggcgtcgt
    56461 cggcagccat ttcattcagc actttcaaaa cgcagcccag cgcgtccgga acgtatccta
    56521 agtctccgct ggcgatttcc gcgtaccgct tgcgtattaa ctcacaatat ttttccacat
    56581 gccctcctgt cagcacactg acttaaccgt ggatgcaagt ctaagcctac gaagataaac
    56641 tctgtttcgc aaggtgacta taccacactc atttctgcaa tatcagcgcc gcaactgcac
    56701 gtattccgtt acaatggcct cctgattcga aaggagtttt cttatggcgc ttaaagcgac
    56761 aatttataaa gcgacggtta atgtggccga tctcgaccgc aaccagtttc tcgatgcctc
    56821 tctgacgctg gcgcgccacc cttcagaaac ccaggagcgt atgatgctgc gcctgctggc
    56881 gtggctgaaa tatgctgatg aacgtctgca atttacccgt ggtttgtgtg ccgatgatga
    56941 gccggaagcg tggctgcgta acgatcacct gggcattgat ttgtggattg agctggggct
    57001 gccggatgag cggcggatta agaaagcctg cacccaggcc gcagaagtgg cgctgtttac
    57061 ctataatagt cgggcggcgc aaatctggtg gcagcaaaat cagagcaaat gtgtgcagtt
    57121 tgccaatctt tccgtctggt atctggacga tgaacaactg gcgaaagtaa gcgcctttgc
    57181 cgatcgtacc atgacgctgc aggcaacgat tcaggatggc gtgatctggt tatcggacga
    57241 taagaataat ctggaagtta acttaaccgc ctggcaacaa ccttcatgat tgtgatttcc
    57301 cgacatgttg ctattcccga tggtgagctt gagatcaccg ccattcgtgc gcagggcgcg
    57361 ggcgggcagc atgttaataa gacctcaacg gccattcatc tgcgttttga cattcgggcg
    57421 tccagcctgc cagagtatta caaagagcgt ctgctcgccg ccagccatca tttgatcagc
    57481 agtgatggcg tgattgtcat taaggcacag gaataccgca gtcaggaact gaaccgcgaa
    57541 gccgcgctag cccggctggt ggcagtgatt aaagatttaa caacagaaca aaaagcccga
    57601 cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc tggcatcgaa agcacaaaaa
    57661 tcaagcgcga aggcgatgcg cggcaaagta cgcagcggtc gggaataaaa agaaggaata
    57721 gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc agcctcttta ctctgatggg
    57781 atgtaataac cgggccgaag tcgatacgct ttctccagca caggctgccg aactgaaacc
    57841 gatgccgcaa agttggcgcg gcgtgctgcc gtgtgctgat tgtgaaggaa tcgaaacctc
    57901 tctgttcctc gaaaaagacg gaacatgggt gatgaatgag cgttatctcg gtgctcgtga
    57961 agaaccttcc tccttcgctt cctacggtac atgggcgcga accgctgaca agctggtatt
    58021 aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa ggcgatgcgc tggagatgct
    58081 cgatcgtgaa ggcaatccga ttgaatcgca gttcaactat acgctggaac cggcacaatc
    58141 cagtttacct atgacgccga tgaccctgcg aggcatgtat ttttatatgg ctgatgcggc
    58201 gaccttcact gattgcgcga ccggaaaacg tttcatggta gcgaataacg cagagctgga
    58261 gcgtggctac ctggctgcgc gcggtcacag tgaaaaaccg gtgttactgt cagtagaagg
    58321 tcactttacg cttgaggcta atccggatac tggtgcgcca accaaagtat tagcgcccga
    58381 tacggcgggt aaattttacc cgaataagga ttgcagtagt ttggggcagt aaacctgctt
    58441 aaggcagaaa caaacgcaaa acaggccaga aggatatatt tcaacatttt gaatttgcac
    58501 gttttttgta ggccggataa ggcgtttacg ccgcatccgg caatggtgct caacgcctga
    58561 tgcgacgctg gcgcgtctta tcatgcctac aacccccctc ataccttaag gcggataagg
    58621 catttacttt atcaccgggt caaaccaaca aagcgacccg ccttgatata aatccctggc
    58681 ttcaggtgcc caacatactg taactgcgtt tctggcttaa acgcagagac atcaccaata
    58741 cgcatatgca tcagatcggc agggctaatc cagcccgatt gcgccagtgt aagtggatgt
    58801 ccggctttgg caaatgcatc agtgacaaat tccgaacaaa accacgactt tttgtctcct
    58861 tcgcccacac tgcttaactg cgctttcgcc aggccgctga cgcactgctg gcgaaaatcc
    58921 tcggagaact gattcagtga gcacatctgg cgagtcacca taaagggaat aaattcgaca
    58981 atgccgcgat agttataacc gctatctttg attttattgg caaacgcggt gatatctgtg
    59041 gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa gcttatcact atgcttcatc
    59101 gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac gttattatca
    59161 cccagaaaga ttgcaacgtg actcacagag gaagtgctga agacgcggat tccaaatgag
    59221 gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt
    59281 tcttttattg attgttcggt gaaagagctt tgatgctgga atttgacagc ccatgttttt
    59341 gcctcggcat ctactgctgt ggctgatggg gctggctgat atcaacggta caagccgaaa
    59401 gtaaaagaaa gctggggaga agcaggcggc agtacgcctt tggtttatcc attttataca
    59461 atccatgtaa aaaagggccc tgaaattcag gaccctttct ggcatcagcc tttaatctgt
    59521 ttcaccagat attcgacgat gtcaccagtc ttaattaact gtttctcgcc gttgcgacga
    59581 tatttatatt cgatatcgtc gttgtcgagg ttacggtcgc ccagcacaat agtgtgcgga
    59641 ataccgatca gttccatatc agcaaacatc acgcccggac gctctttgcg gtcatccagc
    59701 agcacttcga taccttgtgc acgcagttcg ctgtacagtt tctcagcaag ctcttgtacg
    59761 cggaaggatt tgtgcatgtt catcggcaga atcgccacct ggaacggcgc gatagcgtca
    59821 ggccatacga tgccgcgttc gtcgtagttc tgctcaatcg ccgcagctac cacacgcgtt
    59881 accccgatac cgtagcaacc catcgtcagg atttggttac ggccatcttc accctgtacg
    59941 gaggctttca gtgcttcgga gtacttggta cccagctgga agatgtgacc aacttcgata
    60001 ccacgtttga tcagcagcgt accctggcca tccgggcttg gatcgccagc caccacgtta
    60061 cggatatctg caacttccgg ggtagcgaca tcgcgatccc agttgatacc gaagtagtgt
    60121 ttaccatcga tgttagcacc agcagcgaaa tcactcatcg ccgcaacggt acggtcaatc
    60181 accaccggaa tcggcatgtt taccggaccc agtgaacccg gaccggcttt aaccacagca
    60241 cgaatttctt cttcggtcgc gaaagtcagc gggcttgcaa cctgcggcag tttttctgct
    60301 ttaacttcgt tcagctcgtg atcaccgcgc accagcagcg caaccagcgg gaagctgctg
    60361 ccttcaaccg ctttaaccag cagagtctta accgttttct caatcggcag attgaactgt
    60421 tcaaccagtt ccgcgatggt tttcgcgttc ggcgtatcaa ccagcgtcat ttcctgggta
    60481 gcagcagcgc gcggttcttt cggcgcgata gcttctgcca gttcaatgtt cgctgcatag
    60541 tcagaggtgt cggagaagac cacatcgtct tcaccgctct gcgccagcac ctggaattcg
    60601 tgagaggcgc tgccgccgat agaaccggtg tcggcttgca cggcgcggaa atccagcccc
    60661 atgcggctga agattttgct gtaggccgca tacattgcat cgtaggtttc ctgcagggat
    60721 tcctgagaag tatggaaaga gtaagcatct ttcatcagga attcgcggga acgcatgacg
    60781 ccgaaacgcg gacgcacttc gtcgcggaac ttggtctgga tctgatagaa gttcagcggc
    60841 agctgtttgt aagagctaag ctcgttacga atcaggtcag tgataacttc ttcatgagtt
    60901 gggccgagta cgaacggacg ctcgccacgg tcaacaaaac gcagcagttc cggaccgtac
    60961 tgttcccaac gaccactctc ttgccacaaa tcggctggct gaaccaccgg catcgacacc
    61021 tcgattgcac cggcgttgtt catctcttca cgcacgatgt tttcgacttt tttcagaacg
    61081 cgcacgccgg tcggcagcca ggtatataac ccggaggcca gcttgcggat catcccggcg
    61141 cgcagcatca gctgatggct gatcacctcg gcgtcggcag gtgtctcctt gagagtggag
    61201 agcaggtatt ggctagtacg catgttgtta cggttccagt tggaaggtag aacaggctca
    61261 aggcgagcct gggacaaaaa aagtgattta gtttaccagt gcaaaagaaa tgtcaaaaga
    61321 gaagggcgtg aatttaacgc ggttccagcg caaagacttc aaaacctgcg tcggtgacgc
    61381 gccagcgaac gttaaaatca tgtagccaga cggcataggt tttgcccgtt tcctcacctt
    61441 tatgataggc cgggcgcggg tcctgcgcca gtacttcgcg gataaacagc gttaactgcg
    61501 gataacgctt ctccagcgtc aaaagctgct tttcgacctc tgcggtaaaa ctcaccgcca
    61561 tctctgcagc tggcgcactt tgcgcatagc tggcactggc atcgggaagc gattcggcaa
    61621 aggggagata cggtttgata tccactaccg gcgtaccatc gaccagatcc agactgccga
    61681 gcttcagaat cacgctgtct ttatggcaaa caacctcttt cagctctacc agcgacatac
    61741 caattgggtt agggcggaaa gtagagcgtg ttgcgaaaac ccccattctg gcgttaccgc
    61801 cgaggcgcgg tggacgcaca gtcggacgcc agccgccttc catcgtttga tgaaagacga
    61861 aaaggatcca taaatggctg aacgcttcca ggccgcgaac ggcgtcggcc tggttgtagg
    61921 gagcaatgag atgcagttcg ccgttggcgc tttttaccag acctggctgg cgcggaacgg
    61981 cgaatttttc tttatagggc gagcgaataa cgcctatttg ctcgaactgg aaactgctca
    62041 tttcgccgta atgttaagcg cagagccgat acatacagcc tgacgatagc agcctggcgt
    62101 accgctggtg acttcgcagc tatgcagtaa taccgcattg gctttcattt tagaggcgtt
    62161 gatttgcatc cgcttacgcg cggttggaat gctcggcgga gagtcctgat tagaggcctg
    62221 gcaagagtcg ccactgactt caccgagatc gcggaacggt ttgccgacta attcttctgc
    62281 attggtataa attcggaccg gcgtggcgcg cggcgctttc ggttttgcag gctccgcttt
    62341 cggctggggt gcagtgcttt gaacgggttc gacaggggat ctgcttaaca tggaacagcc
    62401 gcttagcatg agtgctacta aacagatcgg taaagcacgc atagtatttc ctcaatgtat
    62461 gatcaaaacg tcaatattga atcaggagct tgtaaaaatg acaagacggg caagtgccca
    62521 tcctgaatga tattacaaat tgtggaaaca gcctaaaaat taccagcctt taacagcgcc
    62581 gccgttaaac actttgtttg ctgcttcgta aacttcatca gactgataag cctggacgaa
    62641 tttcttcacg ttctcggcgt ctttgttatc ttcacgcgtt acgatcaggt ttacgtacgg
    62701 ggagtcttta tcttcgacaa agataccgtc tttcgctgga gtcaggccaa tctggctggc
    62761 ataggtggta ttgataactg ccagagcgat ttgtgcgtcg tccagagatc gaggcagctg
    62821 cggtgcttcc agctcaacaa ttttcagatt ttttgggttc tcaacaacat caagtacggt
    62881 cggcagcagg ccaacgccat ctttcagttt gatcaagccc actttttgca gcagcagcag
    62941 tgaacgacca aggttagttg gatcgtttgg cacggcaacc tgcgaaccat cctgcagttc
    63001 atccagtgat ttgattttct tggagtaacc agcaatcgga taaacaaaag tgttgcctac
    63061 tgcgaccagt ttgtagccac gatctttcag ttgctgatca aggtacggtt tatgctggaa
    63121 ggcgttggcg tcgatatcgc ctttgctcaa tgcttcgttt ggcagaacat agtcgttgaa
    63181 ggttaccagc tcaacgtcca ggccatattt gtctttcgca actttctgcg cgacttctgc
    63241 aacctgctgt tcggcaccaa caatcacgcc gactttaatg tggtttggat ctttttcatc
    63301 ctgaccgcag cctaccagtg ccagtgatcc aatcagggct cccactgccg caaaggtttt
    63361 gaatttgaac gccatacctt attccttctt caattattta tgttgtgttg aacgttactt
    63421 gcgagtgaca gcccggacga tgcggtcgcc tgcgaactga attaaataaa ccagaatgac
    63481 cagcaatacc agtaccgtat tcatcaccgt cgcgttatag ccgatgtagc catactgata
    63541 gccaatctga cctaaaccac cggcaccgac agcaccaccc atcgcggaat aaccgactag
    63601 ggtaatcagg gtgatagttg ccgcattcac cagacccggc aatgcttccg gtaacagcac
    63661 tttacggacg atctgcatcg gcgtggcacc cattgcacgg gaagcttcaa ttaacccggt
    63721 tgggatctcc agcagagcgt tctcgaccat acgggcaata aacggtgctg caccaacggt
    63781 taacggaaca atcgctgcct gcaaaccaat cgatgtacca acaataacgc gggtaaacgg
    63841 aatcatccat accagcaaga taatgaacgg gatagaacgg aaaatgttca caatcgcaga
    63901 aacggtacga tacagcttcg cgttagcaat aatttgcccc ggacgcgtga cataaagcag
    63961 aacgccaacc ggcagaccaa tcacaaagcc aaaaaaaccg gatacgaagg tcattgccag
    64021 cgtttcccat acgccacgaa ccagcagcca catcatcggc tcagacataa cccagtacct
    64081 ctacttttac atggtgttcc tgcagccagg caatggcggc ttgcgtatct tgttgtgtgc
    64141 cgtgcatttc agtcagcatg atgccgaact taacgccacc ggcgtagtcc atctgcgcgc
    64201 taataatgtt gttgttgacg ttgaaacgac gcgcggtttc agaaagcagt ggggcatcga
    64261 ccgattgacc ggtaaactcc agacgcagca tcggcacgca gtcagtaaat ggctccgctt
    64321 gcagacgttc ctggtaatct tccgggatat ccagatgcag ggtcgactga ataaacttct
    64381 gcgccagcgg cgttttcgga tgcgagaaca cttcacttac cgtgtcctgc tcgatcagtt
    64441 cgccattgct gatgaccgcc acgcaatcac aaatacgctt cacaacgtcc atttcgtggg
    64501 tgatcaacag aatcgtcaaa cccagacggc ggttaatgtc tttcagcagt tcgagaatag
    64561 aacgtgtcgt tgccgggtcc agcgcgctgg tggcttcatc acacagcaat actttgggat
    64621 tgctggctaa tgcacgggca atcgccacac gttgtttctg cccaccggaa agattcgacg
    64681 ggtagctatc atgcttatcg ccaagaccaa ccaatgacag caattccgtc acgcgacgtt
    64741 tgatctcgtc tttcggtgtg ttgtccagct ccagcggcag agccacgttg ccaaaaacag
    64801 tacgcgaaga gagcaggtta aaatgctgga aaatcatacc aatctggcgg cgagctttgg
    64861 tcaactcgga ttctgacagc gtggtcagtt cctggccatc gaccagcacg ctaccctcgg
    64921 ttgggcgctc cagcaggttt acacaacgta taagcgtact cttacccgcg cctgaggcac
    64981 cgataacgcc ataaatttgt ccagctggca catgcaggct gacgttgttc aacgcctgga
    65041 tggtgcgggt gccctggtgg aacactttgg tgatattcga aagttttatc attgattatt
    65101 tattatcgtc attaagttag tcgtggcatc tcgaatgcct gaaacgggca acgccgtcaa
    65161 tgaaatggat gttaaggcat ccagacgtct aaatcaatca ggtttatgcg aagagcactt
    65221 tcttgcaggt cgaaacatgc gatactagcg tcacatgcct tattaaggag ctataaaagg
    65281 tggcaaagag cgtacccgca atttttcttg accgtgatgg caccattaat gtcgatcacg
    65341 gctatgtcca tgagatcgac aactttgaat ttatcgacgg tgttattgac gccatgcgcg
    65401 agctaaaaaa aatgggcttt gtgctggtgg tagtaaccaa ccagtctggc attgctcgcg
    65461 gtaaatttac cgaagcacag tttgaaacgc tgaccgagtg gatggactgg tcgctggcgg
    65521 accgagatgt cgatctggat ggtatctatt attgcccgca tcatccgcag ggtagtgttg
    65581 aagagtttcg ccaggtctgc gactgccgta aaccacatcc ggggatgctt ttgtcagcac
    65641 gcgattattt gcatattgat atggccgctt cttatatggt gggcgataaa ttagaagata
    65701 tgcaggcagc ggctgcggcg aacgtgggaa caaaagtgct ggtgcgtacg ggtaaaccta
    65761 ttacgcctga agcagaaaac gcggcggatt gggtgttaaa tagcctggca gacctgccgc
    65821 aagcgataaa aaagcagcaa aaaccggcat aatgattaaa agatgagcga ttgaaataaa
    65881 aatgcatttt tccacttgtc ttcctgagac gactccctat aatgcgcctc catcgacacg
    65941 gcggatgtga atcacttcac acaaacagcc ggttcggttg aagagaaaaa tcctgaaatt
    66001 cagggttgac tctgaaagag gaaagcgtaa tatacgccac ctcgcgacag tgcgctaaag
    66061 tgcgtcgcaa ctgctcttta acaatttatc agacaatctg tgtgggcact cgaagatacg
    66121 gattcttaac gtcgcaagac gaaaaatg
//
LOCUS       DAJTNN010000004        64534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTNN010000004 DAJTNN010000000
VERSION     DAJTNN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.004"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..414)
                     /locus_tag="OO150_000239"
     CDS             complement(<1..414)
                     /locus_tag="OO150_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5514512.1"
                     /translation="MFYSPSLNIFVNPALKDDYINANSWPDDALAVSDDVYNEFAINT
                     PPDDKIRVAGKNGLPTWALIPPPSYEELIQQAESERQLLLNQANEYMNSKQWPGKAAI
                     GRLKGEELAQYNLWLDYLDALELIDTSSAPDIEWPT"
     gene            complement(416..802)
                     /locus_tag="OO150_000240"
     CDS             complement(416..802)
                     /locus_tag="OO150_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5514513.1"
                     /translation="MPVSHYLCIFLNVGLGELSLAGTASGVIGLNGYVTIPLIISGSR
                     RTLIIQWGQARFGGSGGEDAGYLNDFPFAFPSACYGMIVSHVGHTPSGAGILSASAIT
                     SNQFRGFSSIATAANAVLGRYIAIGV"
     gene            833..1399
                     /locus_tag="OO150_000241"
     CDS             833..1399
                     /locus_tag="OO150_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5514514.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            1542..2714
                     /locus_tag="OO150_000242"
     CDS             1542..2714
                     /locus_tag="OO150_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCR5514515.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            2724..3239
                     /locus_tag="OO150_000243"
     CDS             2724..3239
                     /locus_tag="OO150_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCR5514516.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            3294..3596
                     /locus_tag="OO150_000244"
     CDS             3294..3596
                     /locus_tag="OO150_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5514517.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            3611..3730
                     /locus_tag="OO150_000245"
     CDS             3611..3730
                     /locus_tag="OO150_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR5514518.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            3723..6800
                     /locus_tag="OO150_000246"
     CDS             3723..6800
                     /locus_tag="OO150_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5514519.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISANDGASATARITRETMRYNRQLSEQEAR
                     LRRVGEQQRKMHAARGAYARRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQGAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTATVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAATGTVLRMLASGPLAMLRVALYAVSGLLGALLSLIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAATPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     QRIEAPMQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            6797..7282
                     /locus_tag="OO150_000247"
     CDS             6797..7282
                     /locus_tag="OO150_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5514520.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            7279..8379
                     /locus_tag="OO150_000248"
     CDS             7279..8379
                     /locus_tag="OO150_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCR5514521.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPLDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            8470..8688
                     /locus_tag="OO150_000249"
     CDS             8470..8688
                     /locus_tag="OO150_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5514522.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(8923..10608)
                     /locus_tag="OO150_000250"
     CDS             complement(8923..10608)
                     /locus_tag="OO150_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5514523.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            10878..11255
                     /locus_tag="OO150_000251"
     CDS             10878..11255
                     /locus_tag="OO150_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5514524.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(11285..11542)
                     /gene="grxA"
                     /locus_tag="OO150_000252"
     CDS             complement(11285..11542)
                     /gene="grxA"
                     /locus_tag="OO150_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5514525.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            11702..11989
                     /locus_tag="OO150_000253"
     CDS             11702..11989
                     /locus_tag="OO150_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5514526.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            11973..12695
                     /gene="nfsA"
                     /locus_tag="OO150_000254"
     CDS             11973..12695
                     /gene="nfsA"
                     /locus_tag="OO150_000254"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5514527.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGPCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            12756..13658
                     /gene="rimK"
                     /locus_tag="OO150_000255"
     CDS             12756..13658
                     /gene="rimK"
                     /locus_tag="OO150_000255"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5514528.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            13746..14222
                     /locus_tag="OO150_000256"
     CDS             13746..14222
                     /locus_tag="OO150_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5514529.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            14573..15685
                     /gene="potF"
                     /locus_tag="OO150_000257"
     CDS             14573..15685
                     /gene="potF"
                     /locus_tag="OO150_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5514530.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHNPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            15780..16913
                     /gene="potG"
                     /locus_tag="OO150_000258"
     CDS             15780..16913
                     /gene="potG"
                     /locus_tag="OO150_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5514531.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            16923..17876
                     /gene="potH"
                     /locus_tag="OO150_000259"
     CDS             16923..17876
                     /gene="potH"
                     /locus_tag="OO150_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5514532.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVAAAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            17873..18718
                     /gene="potI"
                     /locus_tag="OO150_000260"
     CDS             17873..18718
                     /gene="potI"
                     /locus_tag="OO150_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5514533.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            18778..19266
                     /locus_tag="OO150_000261"
     CDS             18778..19266
                     /locus_tag="OO150_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5514534.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            19307..20464
                     /gene="rlmC"
                     /locus_tag="OO150_000262"
     CDS             19307..20464
                     /gene="rlmC"
                     /locus_tag="OO150_000262"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5514535.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQSEAAGAASDC"
     gene            complement(20608..21339)
                     /gene="artJ"
                     /locus_tag="OO150_000263"
     CDS             complement(20608..21339)
                     /gene="artJ"
                     /locus_tag="OO150_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5514536.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(21630..22298)
                     /gene="artM"
                     /locus_tag="OO150_000264"
     CDS             complement(21630..22298)
                     /gene="artM"
                     /locus_tag="OO150_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5514537.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(22298..23014)
                     /gene="artQ"
                     /locus_tag="OO150_000265"
     CDS             complement(22298..23014)
                     /gene="artQ"
                     /locus_tag="OO150_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5514538.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(23021..23752)
                     /gene="artJ"
                     /locus_tag="OO150_000266"
     CDS             complement(23021..23752)
                     /gene="artJ"
                     /locus_tag="OO150_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5514539.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYNSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(23770..24498)
                     /gene="artP"
                     /locus_tag="OO150_000267"
     CDS             complement(23770..24498)
                     /gene="artP"
                     /locus_tag="OO150_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5514540.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(24716..25231)
                     /locus_tag="OO150_000268"
     CDS             complement(24716..25231)
                     /locus_tag="OO150_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5514541.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            25357..25680
                     /locus_tag="OO150_000269"
     CDS             25357..25680
                     /locus_tag="OO150_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5514542.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25677..26507
                     /gene="amiD"
                     /locus_tag="OO150_000270"
     CDS             25677..26507
                     /gene="amiD"
                     /locus_tag="OO150_000270"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5514543.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26504..27514)
                     /locus_tag="OO150_000271"
     CDS             complement(26504..27514)
                     /locus_tag="OO150_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5514544.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     FRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGKL
                     PR"
     gene            complement(27613..29043)
                     /locus_tag="OO150_000272"
     CDS             complement(27613..29043)
                     /locus_tag="OO150_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5514545.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29054..30055)
                     /gene="ltaE"
                     /locus_tag="OO150_000273"
     CDS             complement(29054..30055)
                     /gene="ltaE"
                     /locus_tag="OO150_000273"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5514546.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30092..31810)
                     /gene="poxB"
                     /locus_tag="OO150_000274"
     CDS             complement(30092..31810)
                     /gene="poxB"
                     /locus_tag="OO150_000274"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5514547.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31943..32911)
                     /gene="hcr"
                     /locus_tag="OO150_000275"
     CDS             complement(31943..32911)
                     /gene="hcr"
                     /locus_tag="OO150_000275"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5514548.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVHTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAKAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32923..34575)
                     /gene="hcp"
                     /locus_tag="OO150_000276"
                     /gene_synonym="priS"
     CDS             complement(32923..34575)
                     /gene="hcp"
                     /locus_tag="OO150_000276"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5514549.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34719..35618)
                     /gene="lysO"
                     /locus_tag="OO150_000277"
     CDS             complement(34719..35618)
                     /gene="lysO"
                     /locus_tag="OO150_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5514550.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGSLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36112..36807)
                     /gene="aqpZ"
                     /locus_tag="OO150_000278"
     CDS             complement(36112..36807)
                     /gene="aqpZ"
                     /locus_tag="OO150_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5514551.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37233..38891
                     /locus_tag="OO150_000279"
     CDS             37233..38891
                     /locus_tag="OO150_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5514552.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38888..39844)
                     /locus_tag="OO150_000280"
     CDS             complement(38888..39844)
                     /locus_tag="OO150_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HCR5514553.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLKLTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39995..41110
                     /gene="macA"
                     /locus_tag="OO150_000281"
     CDS             39995..41110
                     /gene="macA"
                     /locus_tag="OO150_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5514554.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAEIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41107..43053
                     /gene="macB"
                     /locus_tag="OO150_000282"
     CDS             41107..43053
                     /gene="macB"
                     /locus_tag="OO150_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5514555.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHMVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALVRE"
     gene            complement(43126..43350)
                     /gene="cspD"
                     /locus_tag="OO150_000283"
     CDS             complement(43126..43350)
                     /gene="cspD"
                     /locus_tag="OO150_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5514556.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43673..43993
                     /gene="clpS"
                     /locus_tag="OO150_000284"
     CDS             43673..43993
                     /gene="clpS"
                     /locus_tag="OO150_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5514557.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44024..46300
                     /gene="clpA"
                     /locus_tag="OO150_000285"
     CDS             44024..46300
                     /gene="clpA"
                     /locus_tag="OO150_000285"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5514558.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46644..46731)
                     /locus_tag="OO150_000286"
     tRNA            complement(46644..46731)
                     /locus_tag="OO150_000286"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46695..46697),aa:Ser,seq:gga)
     gene            complement(46985..47203)
                     /gene="infA"
                     /locus_tag="OO150_000287"
     CDS             complement(46985..47203)
                     /gene="infA"
                     /locus_tag="OO150_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5514559.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47488..48192)
                     /gene="aat"
                     /locus_tag="OO150_000288"
     CDS             complement(47488..48192)
                     /gene="aat"
                     /locus_tag="OO150_000288"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5514560.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48234..49955)
                     /gene="cydC"
                     /locus_tag="OO150_000289"
     CDS             complement(48234..49955)
                     /gene="cydC"
                     /locus_tag="OO150_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5514561.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(49956..51722)
                     /gene="cydD"
                     /locus_tag="OO150_000290"
     CDS             complement(49956..51722)
                     /gene="cydD"
                     /locus_tag="OO150_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5514562.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQCGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51845..52810)
                     /gene="trxB"
                     /locus_tag="OO150_000291"
     CDS             complement(51845..52810)
                     /gene="trxB"
                     /locus_tag="OO150_000291"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5514563.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53355..53849
                     /gene="lrp"
                     /locus_tag="OO150_000292"
     CDS             53355..53849
                     /gene="lrp"
                     /locus_tag="OO150_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5514564.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            53984..58012
                     /gene="ftsK"
                     /locus_tag="OO150_000293"
     CDS             53984..58012
                     /gene="ftsK"
                     /locus_tag="OO150_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5514565.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSAAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQQVAPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            58167..58778
                     /gene="lolA"
                     /locus_tag="OO150_000294"
     CDS             58167..58778
                     /gene="lolA"
                     /locus_tag="OO150_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5514566.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58789..60132
                     /gene="rarA"
                     /locus_tag="OO150_000295"
                     /gene_synonym="mgsA"
     CDS             58789..60132
                     /gene="rarA"
                     /locus_tag="OO150_000295"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5514567.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSSKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60223..61515
                     /gene="serS"
                     /locus_tag="OO150_000296"
     CDS             60223..61515
                     /gene="serS"
                     /locus_tag="OO150_000296"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5514568.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61754..64198
                     /gene="dmsA"
                     /locus_tag="OO150_000297"
                     /pseudo
     CDS             61754..64198
                     /gene="dmsA"
                     /locus_tag="OO150_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64209..>64534
                     /locus_tag="OO150_000298"
     CDS             64209..>64534
                     /locus_tag="OO150_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5514569.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMA"
BASE COUNT        15867 a        16191 c        17381 g        15095 t
ORIGIN      
        1 cgtaggccat tcaatatctg gagcactgga ggtatcaatc agttccagtg cgtccagata
       61 atccagccac aaattatatt gcgccagttc ctcacctttc agacgaccaa tagcggcttt
      121 accgggccat tgtttactgt tcatgtattc gttggcctga ttaagcaata attgcctttc
      181 tgattctgcc tgttgaataa gttcttcata tgatggtgga ggtattagtg cccatgtggg
      241 taatccattt tttcctgcaa cacgaatttt gtcatctggg ggcgtattga ttgcaaattc
      301 attataaaca tcatcactga cagccagagc atcatctggc catgaatttg cattaatgta
      361 atcatcctta agcgcaggat tcacaaaaat gtttaaagat ggactataaa acatattaaa
      421 cccctatagc gatataacga cctaatacag catttgcagc agtcgctatg cttgaaaaac
      481 cgcggaactg atttgatgta attgcagaag ccgacaggat tcctgcgcct gaaggtgtat
      541 gccccacatg actaactatc atcccataac atgctgacgg aaaggcaaaa ggaaaatcat
      601 taagatatcc tgcatcttca ccaccagacc caccaaatct cgcctgcccc cactgaataa
      661 tcagtgttct ccgggaacct gaaataatta acggaatcgt tacataccca ttcaggccaa
      721 tgacacccga tgcagtgcca gccagagata attctcccaa accaacgttt aagaaaatgc
      781 agagataatg gctaactggc atcatccccg gtttttattc aggggattga tcatgcttat
      841 tggctatgta cgcgtgtcaa caaatgacca gaacaccgat ttgcaacgta atgcactgaa
      901 ctgcgcggga tgtgagcgga tttttgagga taaaatcagt ggcactaagc ccgacagacc
      961 ggggcttaaa aaactgctca ggacactatc ggcaggagac acgctggttg tctggaagct
     1021 ggacagactg ggacgcagta tgcggcatct tgttacgctg atagaagagt tgcgccagcg
     1081 tggtgtgaat ttccgaagcc tgactgacag tattgatacc agtaccccaa tgggccgttt
     1141 cttttttcac gtcatggggg ctctggctga aatggaacgt gaactgatag ttgaacgtac
     1201 cagagcaggg ctggctgcag ctcgtgccaa aggcagagta ggtggacgcc gtcctaagtt
     1261 gaccaccgaa cagtgggcac agattggacg tttactcgag gccggagaat caagacagcg
     1321 tattgcactg atttttgatg tgggcgtttc taccatttat agaaaatttc cggcgaatga
     1381 gagcaatgaa tcgccctgag tcagtattat tttgattttt cctgtaagcg gacaaatacc
     1441 gtcattttgt gtgaataacg acacaactgc gcttagctgt ttgtcaggca caatcacttc
     1501 aacatagggc gaagcctaat ccaatcagga ggttcgccac tatggctcag gattaccacc
     1561 acggggtgcg cgttgttgaa gtcaacgaag gcacccgatc cattaccacg gtgagcaccg
     1621 ccatcgtggg tatggtctgc acgggcgatg atgccgatgc aaaaatgttt cctcttaata
     1681 aacctgtgct gatcactgat gtgctgaccg ccagcggtaa agcgggtgag tctggcacgc
     1741 tggcccgttc gctggatgcc atcgctgacc aggcaaaacc cgtgaccgtt gttgtgcgtg
     1801 tgccgcaggg tgaaacggaa gaagaaacca caaccaatat cattggcgca gtgaccgccg
     1861 agggtaaaaa aacaggtatg aaagccctgt tatctgccca gtcacagctc ggcgttaaac
     1921 cgcgcattct cggcgtgcca ggtcacgaca cgaaggcggt agctactgag ttgctgagcg
     1981 tggcgcaaag cctgcgtgga tttgcttacc tgtcagcgta tggttgcaag acggtgcagg
     2041 aagcaatcac ttaccgtgaa aacttcagtc agcgtgaagg aatgctgatc tggcccgact
     2101 ttactggctg ggacacggtg ctgaatgccg aagcaacggc ttatgccacc gcccgtgcgc
     2161 tcggtctgcg cgctaaaatt gatgagcaga ccggatggca caaaagcctg tccaacgtgg
     2221 gcgtgaacgg tgtcaccgga atttctgctg atgtgttctg ggatctgcag gacccggcaa
     2281 ccgatgcagg tctgcttaac cagaacgata tcaccacgct tatccgcaaa gacggtttcc
     2341 gcttctgggg ttcccgctgt ctgagcgatg atccgctctt tgccttcgaa aactacaccc
     2401 gcacggcgca ggtgctgatg gacacgatgg cagaagcgca catgtgggcg gtggacaaac
     2461 cgctgaaccc gtcgctggcc cgcgacatta tcgaaggtat ccgcgccaaa atgcgtagcc
     2521 tggtcagtca gggctatctc attggtggtg attgctggct ggacgagtcg gtgaacgata
     2581 aagacacgct gaaagccggg aaactcacca tcgattacga ctacacgcca gtgccgccac
     2641 ttgaaaacct gatgctgcgt cagcgcatca ccgatcagta cctggtgaat ttctccagcc
     2701 aggtcagcgc gtaaggggac aacatggctt taccacgcaa attaaaacac ctgaacctgt
     2761 ttaacgacgg gaacaactgg caggggatcg ttgagtcgct gacgctgccg aaattcaccc
     2821 gcaaatatga gaagtatcgc ggcggcggta tgcccggagc ggtggatgtg gatctgggac
     2881 tggatgacag tgcactggat acagaatttt ccattggtgg tactgaactg ctgctgttta
     2941 agcagatggg caaagccacg gtggatggca tccagctgcg ctttaccggc tctattcagc
     3001 gtgatgatac cggggaagtg caggccgtgg agcttgtggt gcgtggacgt cacaaagaag
     3061 tagattccgg cgagtggaag acgggcgaaa gcaacactac caaagtgacc agtaccaaca
     3121 gctacgcgaa gctgaccatc aatggtgagg tgctctatga agtggacctg atcaacatgg
     3181 tggaaattgt ggacggtgtg gacctgatgg aagcgcaccg caacgccctc ggcctctgat
     3241 gtatctgaac ggcgcggaat gccgcgccag aacctaattt acaggacaac aaaatgagcg
     3301 ataagcagac tgaaaagacc attcaactgg atactcccat catgcgcggt aaaacagaaa
     3361 ttaccgaaat tgtgttgcgt aaaccgcagt ccggtgcgct gcgcggcaca cgcctgcagg
     3421 ccattatgga tatggatgtg aacgcgatga tgaccgtgat cccccgcatc tccagtccgg
     3481 cactgactgc acaggaaatt gcagagatgg acccggcaga tctcactgcc atgtcggttg
     3541 aggttgtcac ttttttgttg aagaagtcgg tgcttgccgg tttaccgaca gcctgacggt
     3601 tgacgatctg gtggctgata tcgccaccat ctttcactgg ccgccatccg ttactgacgt
     3661 tatgccgctg accgaagtgc tggaatggcg ggataaagcg attcagagaa gcggggccag
     3721 ccatgagtga caataacctg cgtctgcagg tgattcttaa tgcggttgac aaactcaccc
     3781 gcccatttcg atccgcgcag gccagttcaa gagaactggc tgctgctgtc aaaaaatccc
     3841 gcgatgcaat aaagcagctt gatcaggccg ggagcagtct ggacagcttc cgaaagctgc
     3901 aggcagaaaa tcagaaatta ggcgacaggc tgaactatgc ccgccagcgt gcaaatttgc
     3961 tcagtcagga actgggagcg atggggccgc cttcgcaacg tcaggttgtt gctctgggcc
     4021 gtcaacggct ggctgttcag cgcctagaag aacgccagaa aaagctgcag cagcagacgg
     4081 cgcttgtgcg tgcagaactt tatcgtgctg gtatttcagc taatgatggc gccagtgcga
     4141 cggcccgcat tacccgtgaa acgatgcgtt ataacaggca gctttctgag caggaagcga
     4201 ggttacgacg tgtcggggag caacagcgaa aaatgcacgc cgcccggggg gcttacgcca
     4261 ggcgtcttga ggtaagggat cgtattgcag gtgccggagc caccaccacg gctgcagggc
     4321 tggcaatggg cgcaccagtg atggcggcag taaaaagcta taccagcatg gaagatgcca
     4381 tgaaaggtgt ggcaaagcag gtcaatggtc tgcgtgacga taatggcaac cgcactgcgc
     4441 gtttttacga aatgcagggt gccatcaagg ctgccagcga acagttgccg atggaaaacg
     4501 gtgctgtgga cttcgccgca ctggttgaag gtggtgcgcg catgaacgtc gcaaaccctg
     4561 acgacagctg ggaagaccag aaacgtgacc tgctggcctt cgccagcacg gcggcaaagg
     4621 cggcaacagc ctttgaactg ccagcggatg aactgtcaga aagtctgggg aaaatcgccc
     4681 agctctacaa aatccctacc cgcaatattg agcagctcgg tgatgcgctg aactatctgg
     4741 atgataacgc catgtcgaaa ggggcggaca tcattgatgt catgcaacgc ctgggcggtg
     4801 tggctgaccg tctggattat cgtaaagcgg cggcgttggg ttccaccttc ctgacactcg
     4861 gcgctgcgcc tgaggttgca gccagtgcag caaacgcgat ggtgcgtgaa ttgtccattg
     4921 ccaccatgca aagcaagagt ttctttgaag ggatgaatct gctgaaactc aatccagaag
     4981 tgattgaaaa gcagatgacg aaggatgcga tgggaactat ccagcgggtg ctggagaagg
     5041 tgaatgcgct gccacaggat aagcgcctgt ctgccatgac catgttgttt ggtaaagagt
     5101 ttggcgatga cgcagcgaaa ctggcaaaca acctgccgga acttcagcgc cagttaaaac
     5161 tgactgcggg caatgatgcg ctcggttcca tgcagaaaga atccgacatc aacaaggatt
     5221 cactttctgc tcagtggttg ctggtcaaaa ccggagcaca gaacaccttc agcagcctgg
     5281 gggaaacgct gcgccagccg ctgatggata ttctgtacac ggtgaaaagc gtcacggggg
     5341 cgttgcgtcg ctgggtggaa gctaacccgg aactgacggg cactctgatg aaagtagccg
     5401 ctgtggtagc tgccgttacc gtgggcctcg gcaccttagc ggtggcgctg gctgcagtgc
     5461 tggggccgct ggcagtcatc cgtctgggat tctctgtgct gggtatcaaa acgttacctt
     5521 ccgttacggc aacagtaacc cgaaccagca acgcgttgtc ctggctggct ggcgcaccac
     5581 tggcactgct gcgacgcggg cttgcttcat cgggcaacgc tgcaggttta cttactgcgc
     5641 cgttgtcgtc tttgcgccgc acggcatcac tgacgggaaa tgtcctgaaa actgtagcag
     5701 gtgcgccggt tgcacttttg cggtctggat tatccggttt acgtgcggtt gctgtgatgt
     5761 ttatgaatcc actggcggta ctacgcggcg gactggctgc cacaggcacg gtgctgcgta
     5821 tgctcgcatc cggtccactg gcgatgctgc gcgttgccct gtatgccgtc tctggtctgt
     5881 taggtgctct gctcagtctg ataggtcttg tggttactgc actggcgggt gtggcgctgg
     5941 ttgtctggaa atactggcag cccatcaccg catttctcgg tggcgtggtg gaaggattca
     6001 aagcggcggc aggtcctgtc agtgccgcgt tcgaaccgct taagcctgtg ttccagtgga
     6061 ttggcgacaa agtacaggcg ctgtggggct ggtttactga tctgctgacg cccgttaagt
     6121 cgacctctgc cgaactgcag agtgcagcgg caatggggcg acgattcggg gaggctctgg
     6181 cggaagggct gaatatggtc atgcatccgc tggactccct gaaatccggc gtttcctggt
     6241 tgctggagaa acttggcatt gtcagtaaag aggccgcaaa ggcgaaactg ccggaaagcg
     6301 tgacgcgtca gcaacctgcg acggtgaatg cagacggtaa agtgatgatg ccatcgggtg
     6361 gttttccgtc atggggatat ggctttgcgg ggatgtatga cagcggcggg tatatcccgc
     6421 gcgggcagtt tggcatcgtc ggtgaaaacg ggccggaaat tgttaacggc ccggcaaatg
     6481 tgaccagccg gagaaataca gctgcactgg ctgccgttgt tgccggaatg atgggtgttg
     6541 ctgctacgcc agcagagctt ccaccgttgc accctttggc acttcccgcg aaaggcggtg
     6601 aagcgatggt gagtcgtgca gccactgtgc cgcccgttca acggattgag gcaccgatgc
     6661 agatcatcat tcaaacgcag ccaggacaaa gtgcgcagga tattgcgcgg gaggtggccc
     6721 gccagcttga tgaacgtgaa cgcaggctga aggcaaaagc caggagtaac tacagcgatc
     6781 aggggggata cgacgcatga tgatggtgct gggattgtac gtgtttatgc tgcgcaccgt
     6841 gccgtatcag gagctgcagt atcaacgcag ctggcgacat gcggcaaaca gccgggtaaa
     6901 ccgacgcccg tcaacgcagt ttctgggacc ggacaacgac atgctgacgc tttctggtgt
     6961 tcttatgccg gaggtaacgg gcggcaggct gtcgttgctg gctctggagc agatggcaga
     7021 acagggaaaa gcatggcccc tgattgaagg cagcggcacg atttacggta tgtatgtgat
     7081 tgagggactg aatcagacta aaacggagtt tttccgcgac ggtatgccgc gccggattga
     7141 gttcaccctg tcgctaaaac gcgtggatga atccctgtcc gatatgttcg gtgatctcag
     7201 tgcgcaactg aataatctgc agggaacggc aacatctgcc ttaagcgata tcagtaaaac
     7261 ggtgggaggg ctgctgtcgt gaatttcagc tctgaactgc ttaacaaagg caacaaaact
     7321 ccggcattca gcatcagtat tgaaggcagg gatatcacca ctgtgctgga caaccgcctg
     7381 atggggctga cgctgacgga taaccggggc tttgaagcgg accagcttga tctggagctg
     7441 gacgacgccg atggaaaaat cgtgctgccg cgccgtgggg ctgtcattac gctggcgctg
     7501 ggctggaagg gacagccgct tttcccgaaa ggggcattca cggtggacga gattgaacac
     7561 actggcgcac cggatcgcct gactatccgg gcgcgaagtg ctgattttcg tgaaacgctg
     7621 aatacccgcc gtgaaaagtc gtggcacaag accaccgttg gggaagtggt gaaggaaata
     7681 gctgcgcggc acaaactgaa gatggcattg ggtgaagact tgtcggataa actcgtggag
     7741 catatagacc agactaatga gagtgacggc agttttttga tgcggctggc gcgccagtac
     7801 ggtgctattg cgtcggtgaa aaatggcaat ctgttattca tccggcaggg gcagggtaaa
     7861 agcgccagcg gtaaaccact accggtgatc actatcacac gcaaggacgg cgacagtcac
     7921 cgctttaccc tggcagatcg cggagcttac acgggcgtca ttgccagctg gttgcatacc
     7981 cgcgaacccg cgaagaaaga aagcaccacg gtgaagcgta agcgcaggac taagaagcag
     8041 aagaaagagc cggaagcgaa gcagggcgat tacctggtgg gtacggatga aaacgtgctg
     8101 gtacttaatc gcacttatgc caaccggagc aacgccgaac gggcagcgaa aatgcagtgg
     8161 gaacgcctgc aacgcggcgt tgcatcattc tcgctacaac tggcggaagg gcgggcagat
     8221 ctctacacag aaatgcctgt gaaggtcagt ggctttaaac agccgctaga tgatgcggaa
     8281 tggaccatta cgactctgac gcatactgtc agcccggata acggttttac gaccagtctg
     8341 gagcttgaag tgaggattga tgatttcgaa atggaatgat tcttcgcaat ggagaacttt
     8401 taagtttgca aaatggaata atgcggtatc attattgtga atttagcaaa aatggggaga
     8461 actcgaaaaa tgatgatttg cccactgtgt ggaagtgccg cccatactcg cagcagtttt
     8521 caggtatctt cattgaccaa agagcgttac aaccagtgcc agaacattaa ctgcagccat
     8581 acttttgtta cccatgaaac ttttgttcgt tcgattgcaa cgccaaaaga gtcaaatccg
     8641 gttcagccgc atccaatgaa atcaggacag gtggcgctct ctctttgacg ctgccgccat
     8701 tttgtcgcca tcgttaaaaa acagtgcttc taacatcatg attttaaaag gcataaattt
     8761 cagacaacaa aaacccatca accttgaacc gaaatggcgg ggttgatggg ctccacaaaa
     8821 tggggacatc aaagaaaagc agtggcacta attaagactg atgccctgcg gaaaagttct
     8881 gcggttgtgc aaaaaaattt cattttcagg gcaacttcag ttttatccta atcctggcca
     8941 taccatgacg atgattgtcc ctgccagcgt cagcaggacg ttggcgattg cgtaggtgcc
     9001 cgcatagccc agcgcaggga tgttactgcg agctgtatca ctgatgatct ccattgccgg
     9061 cgcgcaggtg cgtgcgccca tcattgcgcc gaacaacagt gcgcggttca ttcgcaatac
     9121 ataagcaccg aacaagaaac agataaccac gggcacaaga ctgacaatta atccggcaat
     9181 caacatctga ccgccaatcg cgcccaggcc gttattaata ccgctaccgg cgctcagacc
     9241 aacgcctgcc ataaacacca tcaagccgaa ctctttcacc atgcttaatg caccttgcgg
     9301 aatgtaaccg aaggtcgggt ggttagcacg cataaagccc agcataattc cggcgaataa
     9361 caacccggca gcgttcccca tgccgaaact gaatgtgctg aactggaagg tgatcatccc
     9421 gatcatcagc ccaataacaa agaaggcgca aaatgccagc aggtcagtga cctggctgtg
     9481 aatcgagata aagccgatgc gatcggcgat ggtttttacg cgacgggcat caccgctgac
     9541 ttgtaaaacg tcacctttgt taagcacgac gttgtcatct atcggcatct caatctggct
     9601 acgaatgacg cggttaagga agcaaccgtg atcggtcaac ttcagttgtg cgagacgttt
     9661 acctacagcg ttatggtttt taacgaccac ttcttcagtg acgatacgca tgtcgagaag
     9721 gtcacgatcg aaaacttctt taccgttacg gaagctggga tcgagtcggg catgggcgtc
     9781 gggatagcct accaacgcta tttcatcgcc catttgtagc acggcatcac cgtctggatt
     9841 tgccagaatc ccgttacgtc gaatacgttc aatgtagcag ccggtttgtc gataaatacc
     9901 cagttcacgc agatttttgc cgtcggtcca ggccaccagc tccgggccga cgcgataggc
     9961 gcggatcacc ggtaaataaa ccttacggtt ggcatcagtg tccaggccac gttcgcgggc
    10021 gatttgctgg gcgctggtct gtaagtcctg atgctgcaat ttcggcaagt aacgcgcacc
    10081 aacaatcaaa ctcaccagac cgattaaata ggttaaggca tacccgaggc tcagattatc
    10141 cagtgccagt gagagctgcc tgctttccat gccggaatga cgcagtgtat cgccagcacc
    10201 gaccagaacc ggtgtcgacg tcatagagcc tgctaacata ccggccgtca ggccaatatc
    10261 ccagccaaac agcttaccta accctaaggc gatcaccagc gcactgccaa ccatcaccag
    10321 tgctaacatt aggtaatttt tcccatcgcg aaaaaaaatg gaaaaaaagt tcggtccggc
    10381 ttcgaccccg acgcagaaaa taaacagcat aaagccaaga ttaagcgcat cggtgttaat
    10441 gctgaaatgt tgttggccta ataacagcga tacgactaaa acgccaatgg aattacccag
    10501 ttggatcgaa ccaagtcgta actttccgag acatagccca agcgcgagga ccacaaataa
    10561 taacagaatg taattcccat ttaacaattc ggcgacgttt atattcacgg aggctaactt
    10621 cttgtttact agtaagctgt tgaaagaaat ggtaatttac gataatgttt tttaccagaa
    10681 ttcagggcgc agattcattc agcgcaccta aacgatagta aagtaacaat atattttact
    10741 agtgtaatca cattaggtat caacggctat atgaattgcg ttggcctata ttagcatgga
    10801 atgcgaagcg gctttatctt actgaacgcc acactggcga aaaatgtgtt cgatagacgc
    10861 agtgtcagga ggaacgagtg aaacataaac aacgttgggc gggggcaatc tgctgttttg
    10921 tcctcttcat tgtggtgtgc ctttttctgg cgacgcacat gaaaggcgct tttcgggctg
    10981 ccgggcatcc tgaaatcggc ttgctgtttt tcattcttcc tggagcagtt gccagtttct
    11041 tttcacagcg tagagaagtc ctgaaacctc tatttggcgc aatgctggcg gcaccctgtt
    11101 caatgctcat tatgcggctg tttttttcac cgacgcgctc attctggcaa gagctggcat
    11161 ggttactaag cgcggtgttc tggtgtgcgc tgggggcact gtgtttctta tttatcagta
    11221 gtttgtttaa accacagcac agaaaaaatc agtaaagccc tcaacgcgag ggcttgtcag
    11281 acgatcaggc gtccagattt tctttcaccc atgcagcaaa atcggtatag ccgccgatat
    11341 gttgctgatc gacaaaaatc tgcggcacgg tttctacggg tttacctgcc ttttgttgta
    11401 gatcttcttt agtgatccct tccgcacgaa tatctacata ctgatactga aaatcatcgc
    11461 gttcattgct caatttctca gccagatctt ttgcacgcac acagtaaggg caacccgaac
    11521 gaccaaaaat aacggtttgc attatttctc tcctcataga tttatgcctg taatgatcac
    11581 gctaaaatgt attcgctgaa agtaggttta acctgttgca ttaattgcta aaagctataa
    11641 ctgttaaaca caatacagtg aaaagtttta gactgaaggc tcactttgca gagggaagcg
    11701 tatgcgcgcg atcggtaaat tgcctaaagg cgtgttgata ctggaattta tcggaatgat
    11761 gctactggcg gtggcgctgc tgtcggtaag cgactccctg tcgctgcctg agccattttc
    11821 tcggccagaa gtgcagattc tgatgatttt tctcggtgtt ttgctcatgc ttcccgctgc
    11881 ggtggtggtt attcttcagg tggcaaaacg tcttgcccca cagctgatga accgtccacc
    11941 gcaatattca cgttcagaaa gagaaaaaga taatgacgcc aaccattgaa cttatttgtg
    12001 gccatcgctc cattcgccat ttcactgatg aacccatttc cgaagcgcag tgtgaggcga
    12061 ttattaacag cgcccgtgcg acgtccagtt ccagtttttt gcagtgtagt agcattattc
    12121 gcattaccga caaagcatta cgtgaagaac tggtgacgct gaccggcggg caaaaacacg
    12181 tagcgcaagc ggcggagttc tgggtgttct gtgccgactt taaccgccat ttacagatct
    12241 gtccggatgc tcagctcggc ctggcggaac aactgttgct cggtgtcgtt gatacggcaa
    12301 tgatggcgca gaatgcatta accgcagcgg aatcgctggg attgggcggg gtatatatcg
    12361 gcggcctgcg caataatatt gaagcggtga cgaaactgct gaaattaccg cagcatgttc
    12421 tgccgctgtt tgggccgtgc cttggctggc ctgcggataa tccggatctc aagccgcgtt
    12481 taccggcctc cattttggtg catgaaaaca gctatcaacc gctggataaa gacgcactgg
    12541 cgcagtatga cgagcaactg gcggaatatt acctcacccg tggcagcaat aatcgccggg
    12601 atacctggag cgatcatatc cgccgaacaa tcattaaaga aagccgccca tttattctgg
    12661 attatttgca caaacagggt tgggcgacgc gctaaaaccg ccacgtcgat gtatgatacg
    12721 cgggcttttg accaggtctg acagagaggt gcagggtgaa aattgccata ttgtcccggg
    12781 atggaacgct ctattcgtgt aagcggctgc gtgaagccgc tatacagcgc ggtcacctgg
    12841 ttgaaattct tgatccgctt tcttgctaca tgaacataaa tcctgcggcg tcttctattc
    12901 actacaaagg ccgcaagtta ccccattttg acgcagtgat cccgcgtatt ggcaccgcca
    12961 ttacctttta tgggacggcg gcactgcgcc agttcgagat gctggggagc tatccgctca
    13021 atgagtcggt cgccattgcc cgggcgcgtg acaaattgcg ttccatgcaa ctgctggcgc
    13081 gtcagggcat cgacctgcct gtcacgggca ttgcgcattc gccggatgat accagcgatt
    13141 taatcgacat ggtcggtggt gcgccgctgg tggtcaagtt ggttgaaggc acgcagggaa
    13201 ttggcgtcgt gctggcggag acgcgtcagg cggcggaaag cgtgattgac gctttccgcg
    13261 gtctgaacgc gcatattctg gtgcaggaat atatcaaaga ggcgcaaggg tgcgatatcc
    13321 gctgtctggt tgttggcgat gaagtggtcg ctgcgattga acggcgggcg aaagagggcg
    13381 attttcgttc caatttgcat cgtggcggcg cggcaagcgt cgccagtatc acaccacagg
    13441 agcgtgaaat cgcgataaaa gccgcgcgaa cgatggcgct ggacgttgct ggtgtggata
    13501 ttctgcgtgc taatcgcggg ccgttggtga tggaagtgaa tgcgtcgccg gggctggaag
    13561 gaatagaaaa aaccactggt atcgacatcg cgggtaaaat gatccgctgg atcgaacgct
    13621 acgctacgac agaatattgc ctgaaaacgg gtggttagtc gcaatcacat tactgatcat
    13681 ggttttgcct gcgctttttg cgtaagctgt gccggtcttt ttatcgaaag aggttgtaca
    13741 aaattatgac atcgctggtc gttcctggtc tggatacgct gcgtcaatgg ctcgatgacc
    13801 tggggatgag tttttttgaa tgtgataact gtcaggctct gcatctgccc catatgcaga
    13861 atttcgacgg tgtctttgat gccaaaatcg atctgatcga taacacgatc ctgttttctg
    13921 ccatggcgga agtccgacct tcagccgtat tgccgctggc ggcggattta tctgccatca
    13981 atgccagttc gctgaccgtg aaagcatttc ttgatatgca ggatgataat ctgccaaagc
    14041 tggtggtttg ccagtcttta tccgttatgc agggcgtaac ctacgagcag tttgcatggt
    14101 tcgtgcgtca gagcgaagag cagatttcga tggtcattct tgaagctaat gcccatcaac
    14161 tgctgttacc gactgacgat gaagggcaaa acaacgttac cgaaaactat ttcctccact
    14221 gataactcct ttcgagcacg cagtcgctgg tgcagtgact gcgcactgca aaattatctg
    14281 ctgtttttaa ccttttctta aagattattt cacttctctt gtgccgattt ggctttatca
    14341 catagagcaa atacgcataa caatttgtta aataccgttt tttaatccga gctatagtct
    14401 caaaccctgg ctaaagttat tcttgcgatg cttttatata gcgagcagtg ctggccggga
    14461 gagagttatc ttttcttaca ccgcgccgat aaaaaatatg cacgtttatt gcatatcttt
    14521 tagtatgaca acttttgttc gtttgttaac gaactttcag aaggaaagag atatgaccgc
    14581 cttaaataaa aaatggcttt cgggtctggt tgcgggtgct ctgatggccg tctctgtcgg
    14641 cacgctcgcg gctgaacaaa aaacactcca catttataac tggtctgatt atatcgcccc
    14701 tgacacggtg gccaattttg aaaaagaaac cggtattaaa gtcgtctacg atgttttcga
    14761 ctctaacgaa gtactggaag gcaaattaat ggcgggtagc accggctttg acttggtggt
    14821 tccatctgcc agctttcttg aacgccagtt gactgcggga gttttccagc cgctggacaa
    14881 aagcaaattg ccggagtgga agaatctcga cccggagctg ctgaagctgg tcgccaaaca
    14941 caatcccgac aataaatttg ctatgcccta tatgtgggcg acgaccggga ttggctataa
    15001 cgttgataaa gttaaagctg tgctgggcga aaacgcgccc gtcgatagct gggacttgat
    15061 cctcaaacct gaaaatctgg aaaaactgaa aagctgcggt gtctctttcc tggatgcgcc
    15121 agaagaagtt tttgctaccg tgttgaatta tctcggcaaa gatcccaaca gcactaaagc
    15181 ggatgattac accggaccgg caaccgatct gctgttaaag ctgcgcccga acattcgtta
    15241 tttccattca tctcaataca ttaacgacct ggcaaacggc gatatttgcg tcgctatcgg
    15301 ctgggcaggt gatgtctggc aggcgtcaaa ccgcgcgaag gaagcgaaga atggagtgaa
    15361 tgtctcgttc tcgattccaa aagaaggggc gatggcgttc tttgatgtat tcgccatgcc
    15421 tgcggatgcc aaaaacaaag acgaagccta tcagttcctg aattacctgc tgcgcccgga
    15481 tgtagtagcg catatttccg accatgtgtt ctatgccaac gccaataaag cagccacgcc
    15541 gctggtgagt gcggaagtcc gtgagaaccc gggtatttat cctccggcgg acgttcgtgc
    15601 gaagctattc actctgaaag tacaggatcc gaaaatcgac cgtgtgcgca cccgcgcgtg
    15661 gaccaaagtg aagagcggaa aataatccgc agccttagat gccgggcggg cgcaccacac
    15721 ccgccggcaa ttcgcaccat catggtgcgc ttgcacacat tcaatgccgg agagcagccg
    15781 tgaatgacgc tatccctcgc ccgcaggcga aaacccgtaa ggcgctgacg ccgctattag
    15841 aaatccgcaa cctcaccaaa tcctacgatg gtcagcatgc ggtcgatgat gtcagcctga
    15901 ccatctacaa aggtgaaatc ttcgcgctgc tgggcgcttc cggctgtggc aagtccacgc
    15961 tgctgcgtat gctggcaggt tttgaacaac cttctgccgg gcagataatg ctcgatggcg
    16021 tcgatttgtc acaggtgccg ccttacctgc gccccatcaa tatgatgttt cagtcttacg
    16081 cgctgtttcc gcatatgacc gtggaacaga acatcgcttt tggcctgaaa caggacaaac
    16141 taccgaaagc ggaaattgcc agtcgggtcc atgagatgct cggcctggtg cacatgcagg
    16201 agttcgccaa acgcaaaccg catcagcttt ccggtggtca gcgacagcgt gtggcgctgg
    16261 cccgaagcct tgccaagcgc ccgaaactat tactgctcga cgagccgatg ggcgcgctgg
    16321 ataaaaagct gcgtgaccgg atgcagcttg aagtggtgga tattctggag cgtgtcggcg
    16381 tgacctgtgt gatggtcact cacgatcagg aagaggcgat gacgatggcg ggacgtatcg
    16441 ccattatgaa tcgcgggaaa tttgtccaga ttggcgagcc agaagagatc tacgaacatc
    16501 caaccacccg ctacagtgct gaatttatcg gttcggttaa tgtctttgaa ggtgtactca
    16561 aagagcgtca tgaggatggg cttgtgcttt attcgcctgg gctggtgcat ccactgaaag
    16621 tcgacgcgga tgcctcggtg gtcgataacg tgccggtaca tgtggcgctg cgcccggaaa
    16681 aaatcatgct ttgcgaagag ccgcccgcca atggttgtaa cttcgcggtg ggggaggtga
    16741 tacacattgc ctatctcggc gatctttcgg tgtatcacgt tcgtctgaaa agtgggcaga
    16801 tgattagcgc ccagctacaa aacgcccatc gtcatcgtaa agggttaccg acctggggcg
    16861 acgaagtgcg tttgtgctgg gaagtggaca gctgtgtggt gctgacggtt taaggagcaa
    16921 agatgaatac acttgaacct gctgcccagt cgaaaccgcc gggcggattt aagctgtggt
    16981 tgtcgcagct gcaaatgaag catgggcgca aactggtcat tgcgttgcca tatatctggt
    17041 tgatcttgct gtttctgctg ccatttctga ttgtctttaa aataagcctg gcggagatgg
    17101 cgcgcgctat tccaccttat accgaactga tggagtgggc tgacgggcaa ctgtccatca
    17161 ctcttaatct cggtaatttt ctgcaactga ccgacgatcc gctctatttc gatgcttatc
    17221 tccagtcgct acaggtggct gcgatttcga cattttgctg tttactgatc ggctatccgc
    17281 tggcgtgggc ggtggcgcac agtaagcctt cgacccgtaa tattttatta ctactggtga
    17341 tcctgccgtc gtggacctcg tttctgatcc gcgtttatgc ctggatggga atattaaaaa
    17401 acaacggtgt gctgaataat tttctgctgt ggctgggggt tatcgatcaa ccgctgacca
    17461 ttcttcatac caatctggca gtttatattg gcattgttta cgcctacgtg ccgtttatgg
    17521 tgctgccgat ttataccgcg ttgattcgta ttgattattc gctagtggaa gcagcgctgg
    17581 atctcggtgc acgaccgctg aaaacgttct ttactgtgat cgtgccgctg actaaaggtg
    17641 ggattattgc cggatcgatg ctggtgttta tcccggctgt gggcgagttt gtgatcccgg
    17701 aactgctcgg tggcccggac agcatcatga tcgggcgcgt gctatggcaa gagtttttca
    17761 ataaccgcga ctggccggtg gccgcggcgg tagcgatcat catgttgctg ctgctaattg
    17821 tgccgataat gtggttccac aaacaccagc aaaaaagcgt gggagaacac ggatgaataa
    17881 tttaccggta gttcgttcgc cctggcggat tgtgattttg ctgctgggct ttacctttct
    17941 ctacgcgcca atgctgatgc tggttatcta ttcgtttaac agctcgaagc tggtgacggt
    18001 gtgggccggc tggtcaacgc gctggtatgg tgagttattg cgcgatgacg cgatgatgag
    18061 tgcggttggt ttaagcctga caattgctgc ctgtgcggca acggcggcgg cgatcctcgg
    18121 gactattgcg gcggtggtgc tggtgcgctt tggcaggttt cgcggatcaa atggctttgc
    18181 ctttatgatc acagcgccgc tggtaatgcc agatgtcatc acgggcttgt cgctgttgtt
    18241 attattcgtc gcgcttgctc atgccattgg ctggcctgcg gaccgcggta tgcttaccat
    18301 ctggctggcg catgtcactt tctgtacggc ttatgtggcg gtcgttattt cgtcgcgtct
    18361 gcgggaactg gatcgctcga tagaagaagc agcgatggat ctcggtgcga cgccgctgaa
    18421 agtgtttttc gtcattacgc taccgatgat catgcctgcg atcatttctg gctggttact
    18481 ggcttttact ttgtcgcttg atgatctggt gatcgccagc tttgtttccg ggccgggagc
    18541 caccacgtta ccgatgctgg tcttttccag cgtgcggatg ggggtgaatc cggaaatcaa
    18601 cgcccttgca acgttaattc tcggtgcggt cggaattgtc ggatttatcg cctggtatct
    18661 gatggctcgc gcagaaaaac agcggatacg cgatatccag cgtgcaagac atggctgaag
    18721 acactaaaat ttgccaacct ggctacataa tgccgcgcat gccgcggcat tgttttcatg
    18781 gaagacgaaa cgttgggatt ttttaagaaa acatcttcat ctcatgctcg cctgaatgtg
    18841 cctgcgctgg tgcaggtagc ggcgctcgcc attattatga tccgtggcct cgacgtgctg
    18901 atgattttca atacgctggg cgtgcgcggt attggcgagt tcattcatcg cagcgtacaa
    18961 acctggagct taacgctggt atttttaagc agtctggtgc tggtttttat cgagatctgg
    19021 tgtgcgtttt cactggtgaa agggcgtcgc tgggcgcgct ggctatatct gctgacacaa
    19081 atcaccgccg caagttactt gtgggcggct tcgctggggt atggttatcc ggagctgttc
    19141 agcattcccg gtgaatcaaa acgtgaaatc ttccatagcc tgatgctgca gaagctgccg
    19201 gatatgctca tcctgatgct gctgttcgtt ccctcgacca gtcggcggtt cttccagttg
    19261 caataatgtg tataatcgtc gcccctgatg atgtgaaggt caatgtatgc agtgcgcact
    19321 ttacgacgcg ggtcgctgtc gttcctgtca gtggataacg cagccgattc cagagcaact
    19381 ctccgctaaa accgccgatc ttaaaaatct gctcgccgat tttccggttg aggaatggtg
    19441 cgcgccggtg tcaggcccgg aacaagggtt tcgtaataaa gcaaaaatgg tggtgagtgg
    19501 tagcgttgaa aaaccactgc tcggtatgct gcatcgcgat ggcacgccgg aagacctttg
    19561 tgactgcccg ctttatcctg cctcttttgc gcccgttttt gcggcgctaa aaccgtttat
    19621 cgcccgtgcg gggttaacgc cctacaacgt ggcgcgtaag cgtggcgaac tgaaatacat
    19681 tctgctgact gaaagccaga gcgatggcgg catgatgctg cgctttgtac tgcgttctga
    19741 aaccaaactg gcacaactgc gtaaggcgct gccgtggtta caggaacaac taccgcaact
    19801 gaaagttatt accgtcaata ttcagccggt acatatggcg attatggaag gggagacgga
    19861 gatctacctg actgaacaac aggcgctggc agagcgtttt aatgacgtac cgctgtggat
    19921 ccgtccgcaa agtttcttcc agactaatcc ggcggtcgcc agccagctgt acgctaccgc
    19981 gcgcgactgg gtacggcagc tgccagttaa acatatgtgg gatctgttct gcggtgtggg
    20041 gggctttggt ttacactgcg cgacgcctga catgcagtta accgggatcg aaattgcgcc
    20101 agaggccatt gcctgtgcaa agcagtcagc cgctgaactg ggcttaactc gtttacaatt
    20161 tcaggcgctg gactccactc agtttgccac tgctcagggg gaggtgccgg agctggtgct
    20221 ggttaacccg ccgcgccgcg gcattggtaa accgctgtgt gattatctct caacgatggc
    20281 accgcgtttt atcatctact ccagctgtaa cgcccaaact atggcgaaag atatccgcga
    20341 actgcctggg tttcgcatcg aacgggtaca gctttttgat atgttcccgc acaccgcgca
    20401 ctatgaagtg ctgacgctgc tggtgaagca atctgaagcc gcaggtgcgg cttcagattg
    20461 ctgatcaggt gcgctttgtt catgccggat gcggcgtgta cgccttatcc ggcctacaaa
    20521 atcatgctaa atcaatacat tgcagagttg atgtgggcca gataagcgca gcgcatcagg
    20581 caatcttact tttgtcatca gtctcaatta ctgcgggaac cactggtcat tgattttctg
    20641 atatgtaccg tcagctttaa ttgctgccag cgcgttattc agtttttcca gcagagcttt
    20701 gttatccgga cgtacagcga tgcccagacc ggtgccgaag tattgcggat cggtcacttt
    20761 ttcagtagca acgcccagtt gcggattggt tttcagccat tcgtttacca ccgctgtgtc
    20821 accaaatacc ccatcaatac gaccattttt cagatcgata aaggcattct gataactgtc
    20881 ataggagaca gttttcactt cggggtgctg atcctgaata tatttctggt gcgtggtgcc
    20941 gttttccatc ccaatacgtt tgcctttcag atcggcaaac gttttgtagg tatctttttt
    21001 ggcaatcacg acggctgagt tttcatagta gggcgtggta aacgatacct gcttgctacg
    21061 ctcgggcgtg atatccatac cggagattac ggcgtcatat tttctgaatt tcagtgacgg
    21121 gatcaggctg tcgaacgcgt gattagtaaa agtacattct gcctgcattt gtttgcacaa
    21181 ggcttttgcc agatcgatat caaagccgac aatctcatta ttagcaccta tagattcaaa
    21241 gggtggatag gtggctgaaa cgccaaaatt gattttctct gcggcagaag caccgaaagt
    21301 aaaggaagca agtaaagcgg caagaactaa ctttttcatg atggaactcc cgtctgtcaa
    21361 tcttatgatt tttggccgtg tctgcggcat gggataacaa tgccatcaag tgaatttata
    21421 tgcaataaat atgattaaat aatttaaatg aaataaaaaa cgacggataa cttagtgagt
    21481 tgtccgtctt cattataaga atttatgcac tatgtaggcc ggataaggcg tccacgccgc
    21541 atccggcaca ggcaccgtgc tgatgtctga tgcgacgctg gcgcgtctta tcagacctac
    21601 aaaccccccg gcgaatgtac gcagctacat taattccgcc gttcgaatgc cagtgctttg
    21661 cgctcgatca gacgcatcat cagcgtcagc aggccgttga cgaccaggta aataatccct
    21721 gccgcaccga acaccattac atcgtaggtg cgtccgtaca acaactggct gtatcccatc
    21781 acttccatca gcgtaatggt gtaagccaga gaggtacttt tgaacaccag caccacttcg
    21841 ttggaataag aagagagcga gcgtttaaag gcatacggca gcaggatcgc cagcgtatct
    21901 tttttgctca ttcccagggc gctacaggac tgccactgac cttctgggat cgcacgaatt
    21961 gcaccgtaaa acagctgcgt ggtatacgcc gcactgttca gcgataacgc aatcagcgca
    22021 cataaccacg gttctgacaa caaatgccac agtgccggat actcctgcaa agtcggaaac
    22081 tggcccggcc cgtaataaat caggaagatc tgcaccagca gcggcgtacc ggtaaacagc
    22141 gtgatataac cccgcaccag ccacaccagc accggcgttt tcagcgtcag gatgatggta
    22201 aaaatcaatg ccagaatcag tgccacaatc agcgaggcaa cggttagcgt caggctggtg
    22261 tgtagccctt tcatcagttc gggtaaatac tcaaacatta gctgggcctc cgctcaaaac
    22321 gtgtcgcgcg caggtcaatg cgtttgagaa tgtactgact gagcagggtg atcaccaggt
    22381 aaatcgccgc cgccacaata taccaggtaa atggttcctg ggtacgagta gcgatgcttt
    22441 ttgtttgcag cattaaatca ttcacactaa tcaaactgac cagcgcggta tctttcagca
    22501 gcaccagcca ctggttaccg aggccaggca gcgcatgacg ccacatctgc ggcatcacca
    22561 gacggaaaaa gatagccgat ttcgacagcc ccagcgcctg accagactcc cactgaccca
    22621 ccggcaccgc tttcaacgcg ccccgaagcg tttgcgaggc ataggcggca tacagcagtg
    22681 acagagcgat gacaccacaa aggaacgggc taacgtcgaa gttctcaatg tccatctgca
    22741 ctgggatctg cacgaaccca agattgatag tgaagccatc cgaaagcgtc agcagcagct
    22801 gcgaggagcc aaaatagata aacagcacca ccagaatttc tggcaggcca cgcagaatgg
    22861 ttaccagcgc tgaacctgcc cacgcgacag gacgccattt ttccgactcc cataccgcaa
    22921 agaacatcgc cagcgccagc ccgacaatca atgcacaaac ggcaaggccg acggtcatcc
    22981 cggcggcgct tgctaaagga aaaaattcat tcatcaggaa ttacttctgg aaccatttgt
    23041 tgtagatggt ttcgtaagtg ccatctttct tcactttttc cagcgcagtg ttgagtttct
    23101 gctgcagctc agtgttgccc tgacgtaccg cgatgccgag gccagtgccg aagtaatctt
    23161 tatcggtcac tttgtcgccc accgccgcca gtttcgggtt atctttcagc cactcagtga
    23221 ccactgcggt gtcaccgaag acgccgtcga tacgcccgtt ttgcagatcc agttttgcgt
    23281 tctggtagct gttatacgga acggtagtga tttccgggtg cttatccata atgaatttct
    23341 ggtgtgtcgt cccgttctgt acgccgactt ttttgccttt cagctgatca acactggtgt
    23401 atttgccttg ctgacccaca aacagggcag agttgtcata gtacggggtg gtaaacagca
    23461 cctgcttttc acgctccgga gtgatatcca tgcccgccat cacggcttct acgcgacgga
    23521 atttcaggct tgggatcagg ctgtcaaacg cctggttaga gaaagtgcag gttgcatcaa
    23581 tctctttaca cagcgcttgt gccaggtcga cgtcaaaacc aacgatctgg ttgtttgcat
    23641 caatcgattc aaacggagga taggatgctt cggtagcaaa acgaatggtt tcggcagctg
    23701 tggcggaaag actaaaacct gcaattaacg cggcaatcag aacttttttc attgttgtta
    23761 tcccgaatct tagtgagaga gatagttttt aaatgcttcg gtttgcggct cggtaaagca
    23821 gctcgcgtcg ccttgttcta cgatatgacc attttccata tacaccactc ggctggcggt
    23881 tttacgcgcc acttcaactt cgtgggtgac gatcacctgg gtaatattcg tttctgccag
    23941 ctcacgaatg atgctgacga tttgtgccgt aatttccggg tccagtgcgg cggtcggttc
    24001 atcgaacagc agtacctgcg gttccatcat caacgcacgg gcaatagcaa cacgctgctg
    24061 ctgaccacca gaaagatgca gcgggtaacg atcgctataa ggtttgagac gcagacgttc
    24121 cagcagtttt tctgcacggg ccagcgcctg atctttactc aaccccagta cacggcaggg
    24181 cgcttcaatc aggttttgct gcacggtcag atgcggccac aggttgtatt gctgaaacac
    24241 catgccaacg ttacgacgca aatcgcgaat cgctttgtca gagggtgttt tggtgaaatc
    24301 gaaatggttg cctgcaatgt tgagcgtacc ggagcgcggc atctcaagca gattgagtac
    24361 acgcagcagc gagcttttac ccgcgccgct ggggccaagt aacaccagcg tttcgccctg
    24421 tgggcaatcc agcgtgatat cgaacagcgc ctgatgcgcg ccgtagaagc aattaatgcc
    24481 gtttaattga atactcattg acactcgtat actggcagtc tgatagctat tgaggtcgaa
    24541 gatagtacct ttgacagaat aattatgcaa tatttctgct ttaaaagtta aaagcaaagc
    24601 gcattattca ataaacatag cacaaaataa cgggggcggt ggtcggcgag cataaatgtc
    24661 ggcattcctc acgaaatgcc ggacaattta cggggtttat tggttgatca aggcgttagc
    24721 gattctcgat ggactgacgg agcgtacccg ccgtggcatg aacgctgccg cctaagtagc
    24781 gtacatcgtc gatcacccaa cactggcctt cctgaatcat taacacttca tcctgccagc
    24841 tctgatcacc ctgttttaga tccacacgca gaggaatatt tcgcgcatca cgattcggga
    24901 ttgttgaggc actggcaaca cgggcgctct ctggcaaggt tgtgcgactg gagaatggat
    24961 cgttggtcag tagttcccga tggttattat cccgggaggc atcgctaagc agtgtcgcca
    25021 gtttgtcgct cagataaggg cgcaaggcgg tgatgtcgtt gctgcggtgc aaaatgcggt
    25081 agtcataaaa ttgctgggcc acgttacccg ggcctccttc aacgcaagga ccactgcgtg
    25141 tgccgttatc tttataagct ggagtgactg tggtgcaggc actgaggagc agtgcgcagg
    25201 ggataagcat tgtcaatttg ctgtagcgca taatgatttc cttataagcg atcgctctga
    25261 aagcgttcta cgataataat gatatccttt caataatagc gtatcagtct gataatgctt
    25321 ttgagatcga aggcttagca aacaaggaga tcgatcatgc aattttctac aaccccaact
    25381 ctggaaggcc agaccatcgt tgaatattgc ggtgtggtga ccggcgaagc gattttaggt
    25441 gccaatattt tccgtgattt ctttgccggt atccgcgata tcgttggcgg acgttccggt
    25501 gcgtatgaaa aagaactgcg taaagcacgg gagatcgcct ttgaggaatt aggctcccag
    25561 gcgcgggcgc tgggggccga tgccgtcgtc ggtattgata tcgactacga aacggtcggg
    25621 caaaacggca gtatgctgat ggttagcgtc agcggtacgg cggtgaaaac gcgtcgatga
    25681 gaagagtctt ctggctggtc gctgccgctc tgttattggc agggtgtgca ggcgaaaaag
    25741 gcattgtcga gaaagaggga tatcagcttg atacccgacg ccaggcgcag gcggcgtatc
    25801 cgcgcattaa agtgctggtg atccactaca ccgcagatga ttttgatagc tcgctggcga
    25861 cactgaccga taagcaggtc agctcgcatt atctggtccc tgcggtacca ccgcgataca
    25921 acggtaaacc gcgcatctgg caactggtgc cggaacaaga actggcctgg catgcgggga
    25981 ttagcgcctg gcgcggggca acgcgcctta acgacacctc tattggcatt gagctggaaa
    26041 accgtggctg gcaaaaatcg gccggagtga aatattttgc cccgtttgaa ccggcacaga
    26101 ttcaggcgct tattccactg gcgaaagata ttattgcccg ttatcacatc aagccggaaa
    26161 acgtagtggc acatgcggat atcgcaccgc agcgcaaaga cgatccgggg ccattatttc
    26221 cctggcagca actggcgcag caggggattg gtgcctggcc ggatgcgcag cgggttaact
    26281 tttaccttgc cgggcgcgcg ccgcacacac cggtagatac cgcgtcattg ctggagcttt
    26341 tggcgcgcta cggttatgac gttaaacctg atatgacacc gcgcgagcag cggcgcgtga
    26401 ttatggcatt ccagatgcat ttccgcccga cgttatataa cggcgaagcg gatgcagaaa
    26461 ctcaggcgat tgccgaagca ttgctggaga aatacgggca ggattagcgc ggcagttttc
    26521 cgtggtcgcg tagccaggcg gcagttttct cgataccttc atccagggtg atgaccggct
    26581 gataacctaa ctcttcctgc gcacgcgtaa tatccagcgt aaagtcaaaa ttcaacttgg
    26641 agacgccgta gtgggtcagc ggcggctctt ttgctgactt gcggaaacgc tccatgctgc
    26701 gggcgatcat atccagcatc gggtagggga cggaacgaat acgacagtca atattcaact
    26761 cgtcgatcag cttctgcacg atgctgcaca gtgtgcgatg ctcgccgttg gtgatgttgt
    26821 acacacgccc ggaaggtagc ttatcgcagg cttcctggct tgccagccac attgcgtgca
    26881 cggcattttc atagtaggtc atatcgacca gcgcactgcc gccatgcggt aacagaatac
    26941 tgccgtagtg gtgcatcata tgcgccagac ggggaataaa gactttatcg tgcggtccga
    27001 acagactttg tgggcgcaga atagtaaagc gcgtttgtgg attcgcctgc gaaaacatat
    27061 tgatcacttc ttcgctggct gctttgctgc gggcaaactc gttggcgaag cggtgagggc
    27121 gaaaatcttc tttaatatcg cgatggtggt gataatcgaa gtacagggag ggggaagaga
    27181 tatgaataaa gttacgcaca ccccaggcga cagcccattc acccaagcgg cgagtggcgc
    27241 gaacgttagc cagatcgaaa gcctgttgtg tcccccaggg tgaggtaaag ctggagcagt
    27301 gccacagcgt atcaatgccc gcgagcatca ctttagcttg tgatgaaacc agctcggtca
    27361 gatccgccgg aacaaactct gcgcccattt tttccagcaa tttgcccatt gcctcgttgc
    27421 gaccggtcgc tcgcacgctg atgcctttct ggcataaaaa ctctaccgcg tttcgaccta
    27481 agccgctggt ggcgccggta accagtacct tcatatcaat ccactgttgt tgagaaaata
    27541 acgtgcgcat tcttccgtga tttcccccat gatgcaatgg gaaacatgaa agaataacgc
    27601 aggttttgtc gattaatctg tgctttgttc tgccagtctg gcgatttgtt ttgccattcc
    27661 gcgaaaaata aacagatgcg cggggatcat caataaccag taaaacagcc ccggcatacc
    27721 gtgcggatgc cagaaagcgc ggacatcgat agtacgatag tcgcctttat cttccaggct
    27781 aaaacacagt cgtcccagcc ccggcgcttt catgccaaat aacaacgtaa gttgtttttc
    27841 cggttcaacg acaatcactt tccagctatc taccgcatcg ccagtctgta aatattcgcg
    27901 ctccgggcgg cctttcgcca gcttatgacc gatcgcgcgg tccatcaacg cccgtgtctg
    27961 ccacaaaata ttgccaaaga aataacgctc tttaccgccg atttggttca ctacctgcca
    28021 taaagcagca aggctggcgg acgttttaac ggtaaacccc gcctgtttgg caaaataacc
    28081 gtactccggt cgccagcggg caaaggcctg agcgtcgtag ccccagtcgc tggagttgac
    28141 cagtttttcc tcctctttca acgtgctacg taccgcgtca tcgaaagcga tcagccgttg
    28201 tgggatgagt gcacgtagcg cggtatcatc cgccagcaga tcgtgtttca gcccctgaat
    28261 caacgccctg gcggtggtgg gcggtacgga agtaatcaca ttgagaaacc acaccgaaat
    28321 ccagcgggtg gggaggggga tggggatcaa ccagcggcgc ttgccgctca ccgccataaa
    28381 atgttcaaac tgttgctgat aactgagcac ctctggtccg gcggcttcga agatgcggtg
    28441 ttcgctggcc ggatgatcta acaacgccac cagatagtgc agcaagtttt ccagcgcgat
    28501 gggcgtggtg cgtgaacgta cccagcgtgg cggcgttaac actggcaggt tgtagaccat
    28561 atcgcgcatg acttcgaacg ccgctgaacc tgcgccaacg ataattccgg ctcgaagttc
    28621 ggtcacaggt acattcgctt cacgaagaat gtccgccgta gcctgacgag cacgcagatg
    28681 atccgactgc tcatgtggcg gggcctgcaa cgaactgaga aagattaatt gcttaactgg
    28741 tacttcacgt agcgcatcgc ggacgttgag agccacctgg cgctcctgag cgataaaatc
    28801 gccgccttcg cccatgctgt gcaccagaaa atagacggta tcgatatcct gcaacagggc
    28861 cggaaggtta tccggccagc tgagatcgac tttatggcaa ctgacgtttg ccagttgcag
    28921 ctttgcaagc ctgtcgacat gacgtgccgc cgccaggatc tgatgccctt gctggctgag
    28981 tgtgcgcacc agatgctgac caatgtagcc actggcaccg agaactaaaa tgcgttgcgg
    29041 cacgtctctc tccttaacgc gccaggaatg cgcgccagtg ggcggcgact tccgccagtt
    29101 gttcgcgcga gacgtcaaga tgcgtcacca ggcggacaat cggcgaggcg ttaatcagca
    29161 cgtttctcac tttcatgtat tcgcctaacg cggcagcatt ttcttccccg acgcgaacaa
    29221 acagcatatt agtgtcctga cgcatcacat ccgcgcctgc ttcacgcagt tgctccgcca
    29281 tccaggcggc gttgtcgtga tcttcctgca accgtgcgac gttatttttc agggcataca
    29341 tcccggcggc agccagaatc ccggactgac gcatcccgcc accggccatt ttccgccagc
    29401 ggatggcacg tttaatgtaa tcacgattac cgacgagtaa tgaaccgaca ggcgtcccaa
    29461 gacctttcga caggcaaatg gtgaacgaat cacaatattg cgtgatctct ttcagttcgc
    29521 agccgtaagc caccacggca ttaaagatgc gcgcaccgtc aacatgcagc gccagattgc
    29581 gctcgcgggt aaattcccat gcttctttca ggtattcacg cggcagcact ttgccgttgt
    29641 gggtgttttc cagactgagt aatttggtgc gggcgaaatg gatatcgtcg ggtttgattt
    29701 tcatcgccac tttatccagc ggtagcgtgc cgtcggcagc cgcgtcgatg ggttgcggct
    29761 gaatgctgcc cagcaccgct gcgccaccgg cttcaaacag atagttatgc gcggcctgac
    29821 cgacaatata ctcttcgcca cgttcgcagt gactgagcag agcgaccagg ttggcctgag
    29881 tgccggtagg cagaaaaatg gcggcttctt taccggaaag ctctgctgca tagtcctgca
    29941 gagcattaac ggtagggtcg tctccgtaaa cgtcgtcccc aaccggggcg gccatcatcg
    30001 cttcgagcat ggcgcggctc ggtcgggtaa cggtatcact gcgtaaatca atcatggcat
    30061 gtccttatta tgacgggaaa tgccaccctt tttaccttag ccagttcgtt ttcgccagtt
    30121 cgatcacttc atcgccgcgt ccgctgatga ttgcgcgcaa catatacaag ctaaatcctt
    30181 tggcctgttc gagtttgatc tgcggtggaa tggctaactc ttctttggcg accaccacat
    30241 ccaccagcac cggaccgtcg atggagaagg cgcgttgcag ggcttcatca acttcagacg
    30301 ctttttctac acggataccc gtaatgccgc acgcttcggc aatgcgggca aagtttgtgt
    30361 cgtgtagttc ggtgccgtca gtcaaatagc caccagcttt catctccatc gccacaaagc
    30421 ccagcacgct gttgttaaag acgacaattt tcactggcag tttcatctgc actactgaga
    30481 ggaaatcgcc catcaacatg ctaaaaccgc catcgccgca catggcgacc acctgacgtt
    30541 ctggctctgt cgcctgcgca cccagcgcct gcggcatggc gttagccatc gaaccgtggt
    30601 taaacgaacc taacaggcga cgcttgccgt tcatttttag ataacgtgcc gcccacaccg
    30661 ttggcgtacc aacgtcacag gtgaaaatag cgtcatcggc ggcaaaatga ctaatttgct
    30721 gcgccagata ttgcgggtga atggctttct cgctcggttt agctaaatcg tccagccctt
    30781 tgcgggcgtc gcggtaatct tccagcgctt tatccagaaa cttgcgatcg gctttttctt
    30841 ccaccaatgg aagcaatgca cgcagagtcg acttgatatc gccgaccagt gccatatcca
    30901 ccttgctgtg agcgccgatg ctggctgggt tgatatcaat ctgaatgatt ttggcatcgg
    30961 tcgggtagaa ggcgcggtag ggaaattgcg tgccgagtag cactaacgtg tcggcgttca
    31021 tcatggtatg gaaacctgac gagaagccga ttaacccggt cattccaaca tcatacggat
    31081 tatcgtattc gacatgttct ttaccgcgca gggcatgaac aataggcgct ttaattttcc
    31141 cggcaaactc aactaactct ttatgcgccc ccgcgcagcc gctgccacac atcagggcga
    31201 tattgctgga ataacgcagc agttgcgcca gtttgcgtaa ctcttcttct tccggcgtca
    31261 cgactggttg tggcgcatga taccagtgcg tggttgcccc ttctggcgca ggttttaacg
    31321 ccacgtcgcc tggtaacacg acaaccgaaa cgccacggtt aagcaccgct ttgcgcatgg
    31381 caatcgccag tacttgtggg atctgctccg ggctggaaac cagctcgcaa tagtgactac
    31441 attcgcggaa tagctcttgt gggtgggttt cctggaaata gccgctgcca atttcgctgg
    31501 agggaatatg agcggcaatc gccagtaccg gaacgtgatt gcggtggcaa tcaaacaggc
    31561 cgttgattaa gtgcaggttg ccggggccgc acgatccggc gcagaccgcc agttctccgc
    31621 taagttgtgc ttcagcgcca gcggcaaagg ccgccacttc ttcgtggcgg gtggacatcc
    31681 actcgatggt gcccatgcga ttaagactgt cactaagacc attcagagag tcgcctgtga
    31741 ctccccagat gcgtttcacc cctgccgatt cgagtgtttt ggcgatataa gctgcaaccg
    31801 tttgtttcat ggttctccat ctcctgaatg tgataacggt aacaagttta gttcatctga
    31861 cggaggggga agggatggga gagaaaggag gcactaacgg ttaaatagcc cgatgaaagg
    31921 aatatcatcg ggcataaggc gattatgcga gaaccaaatc cccctgcgga tggcaggagc
    31981 aggccagtac gtaaccttca gcgatttcgg cgtcggtcag cgtcattgta ctgctcaccg
    32041 tatattcacc ggaaaccact tttgtcttac agcagccgca aacacccgca cggcaggcag
    32101 cgacaaccgg aacgttattg ctttccagcg cctccagtag cgtggtgcca accggggcgt
    32161 aaaattctcg tgccggttgc agtttggtga atttcagacc gctggtcgcc gcttttgcta
    32221 ctggggtgaa gaatttctct ttaaagaaac gcgtcacgcc gagcgctttc acttcctgct
    32281 ctacccaatc catatacgga gccgggccgc aggtcatcac ggtacgtgaa gctaagtcag
    32341 gtacacctgc cagcagttct cgagtgagac gaccagcgat aaagccttcg gtaacgttat
    32401 tttctgccac cagcgttacc ggatagttac gccactcatc ggcgaaaata acatcctgcg
    32461 gcgtatgcac gttgtagatc acccgcacat cggcctgtgg acggttcttc gcaagccagc
    32521 gacgcatcga cataatcggc gtgacgccgc agcctgccgc cagcaacagg aatttatctt
    32581 ctgctttatc gtcgcaggta aattccccca tcgcgtccga aagccagaga taatcaccgc
    32641 gttttacatc gcgcgtcagc cactgggagc cgacaccgtc atcaatccgc cgcacggtca
    32701 gggtgatata ttcactcacg cctggcgtgg aggaaatggt gtaagcacgc agcgtttccg
    32761 ctgagtttcg cacgctgacc agtgcatatt gcccggcgcg atatgggtag taatcgtggc
    32821 aaatcaggga aatcgtccac acatccggcg tttcttgcgt aatgtgatga acctgcatcc
    32881 gccacgggca ttgattcgtt ggcatcgtca tcgacaaact ccttacgcgc tcaacagttg
    32941 cttcatgtct tcttcaacag tggtgataga acgcaggccg aatttctcgt tcagcaccgc
    33001 cagcaggtct ggtgtcagga aaccaggtgc agtcgggccg gtgacgatat ttttcacgcc
    33061 cagagaaagc agcgtcagca gaatgacgat cgctttctgt tcaaaccagg agagcaccag
    33121 cgacagcggc agatcgttca caccacagcc cagtttctct gccagagtga cagccagaat
    33181 aatcgctgag taagcatcgt tacattgacc tgcatctacc agacgcggca gaccttcgat
    33241 atcgccaaac tcaagtttgt taaagcgata tttaccacag gcgagggtca ggatcaggca
    33301 gtcatccggc acgatggtgg cgaaatcggt gaagtagtgg cgctcgccgc gtgcgccgtc
    33361 acagccacca agcaggaaga tatgacgcag tttttcacgg ctcaccagat caatcagcgt
    33421 atcagcagcg ccaagcagcg tctggcgacc aaaacccacg gtgataaggt gcggaatttc
    33481 gctgtacggg aagcctgcca tctgttgcgc ctgggtgata accgcagaga aatcatcacc
    33541 gtccagatga cgcacgccag gccagccaac aatgctgcgg gtccagatac gatcgtcata
    33601 agcgcttacg gttgggtcga tgatgcagtt cgaggtcatc acgatggggc cagggaaacg
    33661 agcgaactcc acttgctgat tctgccagcc gctgccgtag ttaccgacca gatgcttgaa
    33721 tttacgcagc tccgggtagc catgcgcagg cagcatttcg ccgtgggtgt agacattaac
    33781 gcccgtgcct tcggtctgtt ccagcaggtt gtagagatct ttgagatcgt gaccggaaat
    33841 cagaatgcat ttacccgccg tcgctttgac gttgacctgg gttggcgtcg ggtgaccgta
    33901 tttaccggtt tcgcctgcat ccagaatgct catcactttg aagttcatct ggccgatttc
    33961 cattgaacac tcaagaagcg cgttcatatc ggcaggccag gtccccagcc acgccatgat
    34021 tttatggtac tgggcataaa tatcgttgtc gtattgaccg agaacatgcg cgtgttccat
    34081 ataggccgcc gcacctttca ggccatacag gcacagcaga cgcaggccga gaatgttttc
    34141 gccaatcgcc gctttatctt tgttaggggt aaattctgct gcctgacgtt gcagctcgcc
    34201 gagatcatcg ctcaccagtt gcaggtcagc catcgggtta tcgacgcgcg cgttggcatc
    34261 tacagccagg cattgtgctt tcagcgcctc gcgcaaggca atcgcttcac gagcgtagcc
    34321 gacaatacgc ggagaatcga agttaacgtt ggtcagggtt gagaaaaagg cacgtggcgc
    34381 gaagctgtca acatcgtggt tgatgatgcc gtattcacgc gcttttaccg cccaggcaga
    34441 aagcccttgc agcgccgcga tgagtaaatc ctgaaggtca gaagtttccg ccgttttacc
    34501 acacatcccc tgcgcgtatg agcagccgtt tcctgccgga gtacggatag tttgttcaca
    34561 ttgcacacaa aacatgatca caccttttaa agttatattt aatatacatg tttaaggtta
    34621 agacgcttaa cgcggggata aaagggattt ttcatgcaac tttaagggag attgatttag
    34681 cgcaattttg gcggcaggga tctaccgcca gagaggtatt acgcagagaa aaaggcgatg
    34741 aggatcggca ctaacaggct aagaataaaa ccgtgaacaa ttgccgccgg gaccatatcc
    34801 agcccgccag tacgttgaag aacgggcagg gtgaaatcca ttgatgtggc accgcataag
    34861 cccagtgcag tagagcggct gcggcgaatc agcccaggga tcaacataat agcaatcagt
    34921 tcacgggcca gatcattaaa aaacgccgcg ctcccgatta ccggaccaaa agattcggtc
    34981 aataaaatac cggaaagaga ataccagccg aaaccggagg ccattgccag cgcggtattg
    35041 atggggagat caagaataaa ggcgttaatt aaactaccaa ttaatgaact gacaaccacc
    35101 accacggcga caatcattcc ccggcgatta aggacaatct gctttaaggt catgccatta
    35161 ttgcgcaact gaataccaac gaggaaaagt agcaaaatta acgtgtattc actggcttcg
    35221 gtcgcgtgtt gtaagaaagc cagtccactc agaccaatgg caaaaccaat cactactacg
    35281 ccgcacagtt ttagcgactc cagcgccatc gcaatacgcg acgggagttt ttcttgctga
    35341 tggtggttgc gccacggcag gcctcgctcc agccacatca gggcggcaat attacacagt
    35401 aaaataacgg taatactgac ggcagaataa tgcagaatcg ccaacaggtt actggcgagg
    35461 ttatcgagaa acgccagact gatacccata aaaaagagaa taaggtaaac catccagctt
    35521 aatagctgat taataacttt taacgcagct tgttggcgaa gcggaatgag gtaacccaca
    35581 atcaggggaa ccagaatgat taacagccca gaaaacatga aaacccagtc cttgcaaaga
    35641 tgaagtcgaa atgcgcgatg acacactact gaaagcggaa ggacgagtaa agttgcaatt
    35701 aaaaggaaat gttatgcata aggagcagta gagtattcgt tttcatttaa agatattctt
    35761 gcgctttaat tacaaactgc accgatgttg gtggcgtcaa aatcgccgag gcgttccctg
    35821 aaggccgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg atgttacctc
    35881 gcgcccgacc cggtagccgt aagggataag tcgagggcac cgcgcagcgg cgattttgtt
    35941 cgccagagcc cgggggggca gggggcggcg gcgattggcc gccccctgcg cgctccttgc
    36001 gccagtggca atatgtggct tagctcatga aaggagcgca acaagatgat gaatcaacat
    36061 ataacaacat cttaaaaaaa gcctgacatt acgccaggcc ttctgcgtta attaatcacg
    36121 cttttccagc agggtccggt aaatcagacc accgataatg ccgccgacaa ttggcaccac
    36181 ccagaagaac cacagttgtt ctaatgccca gccgccctgg aagatagcaa ccgcggtgct
    36241 gcgcgccggg ttaacagaag tgttagtcac cggaatacta attaagtgaa tcagggttaa
    36301 ggccagacca atagcgatcg gcgcaaaacc tgccggcgcg aatttggcgg ttgcgccgtg
    36361 gatcaccaac aggaaacctg cactcaatac cagttcaact accagcgcgg aaagcatgga
    36421 ataaccgcct ggtgaatgct cgccataacc gttagaagca aaaccgctgg ctgccgcgtc
    36481 aaaacccgtt ttaccactgg caattaaata cagcagcgcc gctgcaacaa taccgccgac
    36541 aacctgggca attacgtagc caacgacttc ttttgccgga aaacgtccgc cagcccataa
    36601 accaatagtg accgccgggt taaaatgacc accagaaata tgaccaacag caaaggccat
    36661 cgtcagaacg gtcagaccga acgccaacgc cacgccggca aaaccaatgc ctaattccgg
    36721 gaagcctgcg gccagtacag cactaccaca gccaccaaaa acaagccaga aagtaccaaa
    36781 acattcagct gctaattttc tgaacatatc cacctcaatt aaaaattgac cctgtgaaaa
    36841 atatggtcgt tttatagggt cgtcgtaaaa aaatgacgac ggaaataatg cgcggctatt
    36901 ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca
    36961 acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcac gcaaaagatt
    37021 taatccttta ggcgtaataa aaaataatat atcatgctaa ttatttgatt ttgttgtttt
    37081 tgcagactta tcagcaagag ggagtataac gcgattattc gctcattttt cagacatttg
    37141 ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgccagcg
    37201 taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg
    37261 gttttcgcgg tatcaaccgt ttgtcgttga tgctggaaca aaacaacgtc ctgattgggg
    37321 agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat
    37381 cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc
    37441 gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg
    37501 ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta
    37561 ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt
    37621 ttctcgataa agacggacat ccgattgatg tcgaggatat taacgatcag gcacgccatc
    37681 tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta
    37741 acggcacggt gccaaatgtc cctaatgtgg aagtcaccgc gcgccagctc gatttcctcg
    37801 cccgtgagtt atcctcacat ccgcaaaatc tctctgatgg gcagattcgt cagggacttt
    37861 ccgcaatggt acagctgctt gagcattatt tctctgagca gggggccgga caggcgcgat
    37921 atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca
    37981 tcaaccggat gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt
    38041 ttgctacttt attgcaggca aaaggcacat tgagactgga taaagacgcg cgtccattgt
    38101 tgctgatcga agatccagaa acccgtttac accccattat gctttcagtt gcctggcatc
    38161 tgttgaatct tctgccattg cagcgcattg ccaccaccaa ctcgggtgag ttgctttcgt
    38221 taacgccggt agagcatgtt tgccgactgg tacgtgagtc ctcgcgcgtt gccgcctggc
    38281 gtctggggcc gagtggcttg agtaccgaag atagccgacg catatccttt cacattcgtt
    38341 ttaaccgtcc gtcatcgctg tttgcacgct gctggttgct ggtggaaggg gaaacggaaa
    38401 cctgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca
    38461 aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg
    38521 ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg
    38581 tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg
    38641 cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg
    38701 cgcaaatccc ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc
    38761 gctcttccaa acccgatctt gccattgaag tggcaataga ggcaggacgt cgtggtgtgg
    38821 actccgtacc gacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc
    38881 gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat
    38941 agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta
    39001 tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc gattcccaga
    39061 aagcgttgta atcagcgtgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa
    39121 tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac
    39181 aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct
    39241 ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc
    39301 ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt
    39361 tgcgaaacag gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagcttaa
    39421 gcgtgaactg ctcgccgttt ttgccttcca gcttcgccag ttgcagcccg ggggtattca
    39481 aatataagct gaattcttcc gccgacatac atccgcggag taacgcataa tggtaacgta
    39541 acgcctccag caattgctta cggctaagat tcgccgcaag gtaagggcga tgcagacgca
    39601 caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt
    39661 tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa
    39721 aactacggcg atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa
    39781 gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat
    39841 tcatgaaatc attggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat
    39901 caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg
    39961 acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa
    40021 gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct
    40081 taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag
    40141 cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gatgtgggcg cgcaggtcag
    40201 cggtcagttg aaaactctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt
    40261 aggggttatt gatcctgaac aggctgaaaa ccagattaag gaggtcgaag caacgctgat
    40321 ggagctacgt gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta
    40381 ttcccgtcag caacgtctgg cacaaacgca ggcggtttca cagcaggatc tcgacaccgc
    40441 cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgg aaatcaagcg
    40501 caatcaggct tctctcgata cggctaaaac caatctcgat tacactcgca tcgttgcccc
    40561 gatggccggg gaagtcacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca
    40621 acaagcaccg aacattctga cgctggcaga tatgagcacc atgctggtaa aagcgcaggt
    40681 ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcttggttta cggtgcttgg
    40741 cgatccactg acgcgctacg aggggcaaat caaggatgta ctaccgacgc cggaaaaggt
    40801 taacgacgct attttctatt acgcccgttt tgaagtcccc aaccccaatg gtttgctgcg
    40861 gctggatatg actgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgctgacgat
    40921 ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg
    40981 taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga
    41041 gattgtcaaa gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc
    41101 tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat
    41161 gagcaggttg aggtgctgaa gggcatcacc ctcgatattt atgccggtga gatggtagcg
    41221 attgttggcg cttcaggttc cggtaaatcg accctgatga atattctcgg ctgtctggat
    41281 aaggccacca gcggcaccta tcgcgtcgcc ggtcaggatg ttgccacact ggacgccgat
    41341 gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttactt
    41401 tcgcatttaa ccgccgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg
    41461 aaacagcggc tgcttcgcgc ccaggagtta ctgcaacgac tggggctgga agaccgtaca
    41521 gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca
    41581 ttgatgaacg gcggtcaggt aattcttgcc gatgaaccaa ccggcgcact ggacagccat
    41641 tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tatggtgatt
    41701 atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac
    41761 ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag
    41821 cctgttgtca acacggtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg
    41881 acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg
    41941 attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa
    42001 atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa
    42061 gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc
    42121 atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg
    42181 cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat
    42241 gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt
    42301 gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat
    42361 gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa
    42421 gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg
    42481 atgtccgggc gagttatggg ccagtcgtgg ctgaactcca ttactgtcag ggtaaaagaa
    42541 ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacgga
    42601 aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca
    42661 cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt
    42721 ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc
    42781 atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg
    42841 gtttgcttgg tcggtggcgc gttgggaata acgctgtcac tgttaattgc tttcaccttg
    42901 cagcttttct tacccggctg ggagattggt ttttcaccgt tggcactgct gctggcgttt
    42961 ctctgctcga cggccaccgg gattttattt ggatggttac ccgcacgaaa tgcggcacga
    43021 ctggatccag tagatgctct ggtacgagag taatttttga gataaaaatg ccagccgatc
    43081 gggctggcat tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc
    43141 ttctacttcg acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc
    43201 aaactgaacg gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata
    43261 atgagcgaaa atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt
    43321 gttgaaccac ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa
    43381 gtaagatgga ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa
    43441 atacaattta gagaattagg gcgagccgtc aagcgtttga caggggccag ggggcaggta
    43501 tgaatcaaaa atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg
    43561 ccaacgatag atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt
    43621 gtcagcggca gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa
    43681 aacgaacgac tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa
    43741 accgccatct atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt
    43801 tattgacgtg ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct
    43861 cgctgttcac taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac
    43921 caaagtggcg atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct
    43981 agaaaaagcc tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact
    44041 ggaactcagt ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac
    44101 cgtcgagcac ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc
    44161 gtgttctgtg gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac
    44221 acccgttctg cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg
    44281 tgtactgcaa cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg taaccggtgc
    44341 aaacgttctg gtcgctatct ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa
    44401 acatgaagtc agccgtctcg atgtggtgaa ctttatctct catggcacgc gtaaagacga
    44461 gccgacacag tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga
    44521 ggaacgtatg gagaatttca cgacgaacct gaatcagctt gcgcgcgtgg gcggaatcga
    44581 cccactgatt ggtcgtgaga aggagctgga gcgtgctatt caggttctct gccgtcgccg
    44641 taaaaacaac ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga ttgcggaagg
    44701 tcttgcctgg cgaattgttc agggcgatgt gccggaagtg atggctgact gtacgattta
    44761 ctctctcgat atcggttctc tgttagcggg cactaaatat cgcggcgact ttgaaaaacg
    44821 ttttaaagcg ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga
    44881 gatccacacc attatcggtg cgggtgcagc gtctggtggc caggtcgatg cggctaacct
    44941 gatcaaaccg ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga
    45001 gttcagcaac attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat
    45061 tactgaaccg tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga
    45121 agcgcaccac gacgtgcgtt ataccgcaaa agcggtgcgt gcagcggtag agctggcggt
    45181 gaaatacatt aacgatcgtc atctgccgga taaagccatt gacgttatcg acgaagcggg
    45241 cgctcgcgca cgcctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat
    45301 tgagtccgtg gtggcccgta ttgcgcgcat tccagagaag agtgtttctc agagtgaccg
    45361 cgataccctg aaaaacctcg gcgatcgcct gaaaatgctg gtcttcggtc aggataaagc
    45421 cattgaggcg ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa
    45481 accggttggt tcgttcctgt ttgccggccc taccggggtc gggaaaacag aggtgacggt
    45541 acagctttcg aaagcgttgg gcattgagct gctgcgcttt gatatgtccg agtatatgga
    45601 acgccatacc gtcagccgtc tgattggtgc gcctccggga tacgttggtt ttgatcaggg
    45661 aggtttgctg actgatgcgg tcatcaagca tccacatgcg gtgctgttgc tggacgaaat
    45721 cgagaaagcg catccggacg tgttcaatat tctgttgcag gtgatggaca acggtacgct
    45781 gaccgataac aacggacgca aagcggactt ccgtaacgtg gtgctggtga tgaccaccaa
    45841 cgccggggta cgtgaaactg agcgtaaatc cattggtctt atccaccagg ataacagcac
    45901 cgatgcgatg gaagagatca agaagatctt tacaccggaa ttccgtaacc gtctcgacaa
    45961 cattatctgg ttcgatcatc tgtcaactga cgtgatccat caggtggtgg ataaattcat
    46021 cgtcgagttg caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc
    46081 gcgtaactgg ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg
    46141 tgtcatccag gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt
    46201 ggacggcggt caggtcaccg tcgcgctgga taaagagaaa aatgagctga cttacggatt
    46261 ccagagtgca caaaagcaca aggcggaagc agcgcattaa tctgattgtt aggtaggttg
    46321 gtcaagtccg taatctcgaa agaggttacg gactttttgt ttatggggtg gaggaggttc
    46381 agaccctttt tttaatgatg atggtaagtt gttgataatt agtgctgcgg gaaggtaagg
    46441 ataaaaaagg gtgctgcagg agaatgggat ggttttgctt tatttacaac gggctaaacg
    46501 tgtagtattt gagttcactg ccgtacaggc agcttagaaa ttcacaggta acatactcca
    46561 cccgcccacc atgttcactg ccgtacagac agataaaatg cgaaaaaaaa gctcgcactt
    46621 tcgtacgagc tcttctttaa atatggcggt gaggggggga ttcgaacccc cgatacgttg
    46681 ccgtatacac actttccagg cgtgctcctt cagccactcg gacacctcac caaattgttt
    46741 tgctaccaaa cctcatgggt ggcaacgggg cgctactata gggagttgga gtaaaacggt
    46801 caagaagaat tttaatgata attattgttt gctcatactg taaacaagtt gtgcagtata
    46861 tctacatcga gacaagttac ggacttatac ttccaaagta cttcatacat atcacaaaat
    46921 aaaaaggccg gttaaaccga ccttttactc gttctttctc ttcgcccatc aggcggtaaa
    46981 acaatcagcg actacggaag acaatgcggc ctttgctcag gtcgtacggg gtcagttcaa
    47041 cagtcacttt gtcgcccgtc aggatgcgga tgtagttttt gcgcatttta ccggagatgt
    47101 gtgcagtaac cacgtgaccg ttttctaact ctacgcggaa catggtatta ggcaacgttt
    47161 caagaacggt accttgcatt tcaatattgt cttctttggc catctaatcc tctggggtat
    47221 cactaccgta atttgaaccg gcaagataat gccgaagttc tgtaaataag taaagatttg
    47281 cgcgctaaat cgcaacaaac aggttcggca cattactccg aaaacacacg gctaagccgc
    47341 accaaaagcg caacgtataa gggagcggtg agataaacga tgggcgttac ctgacgcgaa
    47401 aaattcctta tcggcagcgg ggtaatgagc gtaaccaact ctgcgaccgc aattataaca
    47461 ctctggggag aaatgtgccg aaaacattca ttcttgtggt gaaaacaagc atcgtggtac
    47521 ccagaaatta ttcggcaatc gtccgaggcg catttgattg agataattaa ggtaatcccg
    47581 gcggggaatt tcgcaggcac caagcgatgc tgtgtgatcg ttaaggacct ggcagtcgat
    47641 aagcttaccg ccatgaccga taaattcctc acagaatacc agaagcgccg ttttagacgc
    47701 attttccatc cggctgaaca tggactcgcc acaaaatagc gttccctggg ccacgccgta
    47761 cataccgccg acaagctcat cttcacgcca gacttcaatg gagtgggcat ggccgagttc
    47821 gtgaaggcga tggtaggctt cgaccacgcc acgcgtgatc caggttcctt cttcgcgatc
    47881 gctggcacag ccttcaatga cctgaccaaa agcgtaattc atcgtgacac gatagggcga
    47941 gcgtttatga aatcgcttca tactacggct gatatgcagt gattctggcc atagcaccgc
    48001 gcggggatcg ggcgaccacc agaggatggg gtcgcctgga gaaaaccacg gaaaaatacc
    48061 acgctggtaa gccattaaca ggcgcgcagg gctaagatcg cccccaagtg ccagcaggcc
    48121 gttaggctca cgtaatgcgc cttccgggga agggaaggct attgaatggc gagaaagctg
    48181 aaccaggcgc atgaccgcaa aactccacgc aagtcggatc gttcaataat agcttacaaa
    48241 ccctgcttga actggtaata acgcccctgt ctggcaagca gctctgcgtg agtaccttgc
    48301 tcaataattt gcccgttgtc catcactatt atttgttgga aacgagagag tccgcgaagt
    48361 cgatgggtga ccattaacac cgttttctca cgcatcattt ctgcaagcaa ttcaaggatc
    48421 tggctttcgg ttgtggcatc taagccttcg gtaggttcat ccagcaacac cagtggcgca
    48481 tcatgtaaca gcgcacgggc gatagccaga cggcgcagtt caccaccgga gagctggcgt
    48541 ccgccttcac ctaaccaact gttgagacct gcatcctcga gcagcttctc cagaccaacg
    48601 cgacgcaagc tctccgccag agcctcatca ctactgccag gcgaggcgag taacagatta
    48661 tcacgcagcg tggcgctaaa cagatgcact cgctgaggaa caacgctgat ggtctgtcgt
    48721 agagccgctt cattcaggct ggctatgggg ctatcgttaa gcaaaatctc gccctgttgc
    48781 gggtcccaag cgcgggtcag cagttgtaac agtgttgatt tgccgcatcc ggttcgcccg
    48841 agaatcgcaa tatgttcccc ggcgtttacc tgaagagaaa tccctttaag tgcctgttgg
    48901 gattgctccg gataagtgaa ctgaacatcc cgtaatgtca gcgaaacgcg atcggcaaca
    48961 cgagtttggg tatcagggaa ggtgacttcc ggtttttgat ccgttaaatc agtgatccga
    49021 acggcagagg caatgacttg ccccagatgc tgaaatgcac ccgttactgg tgccagtgct
    49081 tcaaacgcgg ctaacgcgca gaagacaaac agggcaatta acgcgccggg ttgagcattg
    49141 ccgccaacgc cgccagacgc catccacagc atcaggatca ccgctaacgc gccaatgagc
    49201 agcattatcg cttgcgacaa tgcggtcagt tcagattgac ggcgttgcgc ttccagccat
    49261 tgaatttctg tattctctag ttgcgtgcga taacgatcgc tggcaccaaa aatggtcagc
    49321 tcagcttgcc cttgcagcca ggccgtcagt tgttggcgat actgtccgcg aagatgagtc
    49381 agattttgcc cggtgctttt tcccgcacga taaaacagcg gtggcatcag gaaaagcgtc
    49441 agtaacataa tgccgcccag cgtaaaggcg agggtgaaat caaggaaact taacccgatt
    49501 gtcaccatca taatcaccac aaaagcgccc accagcggcg agataacgcg caggtaaaga
    49561 tgatcgagcg tatcaacatc cgccaccacg cgattgagca attcgccctg acgatagcgc
    49621 gccagtccgg caggggagag gggcaacaat ttgctgaagg tgtaaatgcg cagatgctgc
    49681 aacacgcgga aagtcgcgtc gtgacttacc agacgttcaa aataacgccc ggcagtacgg
    49741 gtgattgcgg caccacgcac gcccgcagcg ggtagcatat agttgaagct gtacagtccg
    49801 gcaacccccg caaccgctga ggccgagagg aaccagccgg aaagtgtcaa cagtccgata
    49861 ctggcgagca gcgtcacaat tgccagcaca ataccaagac ttaacatcca tttatgacgt
    49921 ttatacagtg ctagataggg tagcaaagcg cgcatttaaa tctcctcctg acgatgggcc
    49981 agtaatgtgg cgaatgggcc accagccaca cttaattccg cgtaacgtcc ttgctcaata
    50041 atccggccat cctgcataac ccaaatgaca tcccagtcag caagatcttc taactggtgg
    50101 gtgaccatta acgttgtctg gcgcagagag gcggcattca gcgcctccat tacgcgctgt
    50161 tcactgtgag catcaaggct ggcagcgggt tcatccaaca gtaatagcga acagggattt
    50221 agtaacgcac gggccaccgc cacgcgctgc gcctgcccca cggaaaggcg ggcagcctgg
    50281 tcgccaacag gcgtatcaac gccttgtggc aggagcggta gaaactcgct gacccaggcg
    50341 ttatccagcg ctgcttgtaa ttcatgttcg ctggcatcag gtcgcgccag tagtacgtta
    50401 tcccgtaatg ttgctgccgg taattgtgga ttttgcccaa cccaggagag atgtttacgc
    50461 catgattctg gtgataaatc gcgtaattct atcccgttga ttcgtagcga tccctgatat
    50521 gagagaaaac cagaaagcgc gttcaacagt gaacttttac ctgaaccgct gcgaccaacc
    50581 aacacggcac gttggcctgc tggcaaagta aagttcagcg gtccggccag cgttttacct
    50641 tccggcgacg tgataaacag atcctcggcc tcaatggtca ccggatcggt cgatgctaat
    50701 tccacctcgc cacactgcgg atgggcgagc ggggtttcca taaacgtttt cagactgtca
    50761 gctgcaccaa cagcctgggc tttagcatga taaaacgtac cgagatcgcg taatggctgg
    50821 aaaaactctg gcgcaaggat cagggccaga aaacccgcag ccagcgtcac gccagtatcg
    50881 tagtgaccaa aatccagctc gccaagatag gaaaaaccaa agtagaccgc caccagagca
    50941 attgacagcg aggtaaaaaa ttcgagaatg ccggaggata aaaatgccag ccgtagcact
    51001 tccattgtcc gttggcggaa atcttccgaa gcagaacgaa tactttcaat ttcagcttca
    51061 ccacgaccaa aaatacgcaa tgtttccatg ccgcgcaggc gatcgaggaa atgcccactt
    51121 aagcgagcaa gagcgagaaa gttacgtcgg ttagcatcgg cagcccccat tccaaccagc
    51181 gccataaaca acggaattaa cggtgcagtg cccagcagaa tgagcgccgc agcccagttt
    51241 gaggggaaga ttgccaccac aatcagcaac ggcaccgaca ctgccagcgc catttgcggc
    51301 aggtagcgtg catagtaatc atgcatatcg tcaatttgct cgagtaccag cgtcgcccag
    51361 ctccccgcag gtttaccctg aatccacgct ggccctgctt gttgcagacg gtcgagaacc
    51421 tgccggcgga tggcaaagcg gatatgctgc ccggcgtgat aacccacccg ttcgcgtaac
    51481 cagaccaccc atgcgcgcag tacaaaggtc agaaccagta acgtaaaggg aagcagcagg
    51541 gcttcacggg gaatattctc cataatcata tgttgcagaa ttcgcgccat gaaccaggcc
    51601 tgggcaatga tcaatatgcc gctcacaaag cccagcagac gagaaatatt cagccaacgt
    51661 tgggagatga cgctttgctg ttttaaccag cgggttaact ctttttgacg agatttattc
    51721 attgcacgct tagcaggtga gttatcagaa ttatttgcag agcaatgtta caacggggaa
    51781 aaaataaagg cgacccatag tcgcaaggtg tcgccttctt tacttttgtt actgatttgt
    51841 aaaattattt tgcgtcagct aaaccatcga ggtagcgttc cgcatcaagt gctgccatgc
    51901 agcctgtacc ggccgaagta atggcctgac gataaatatg atccatcacg tcacccgcgg
    51961 caaagacgcc aggaatgctg gtctgggtgg cattaccatg aatacccgac tgtactttga
    52021 tgtagccgtt ttccagttcc agctgccctt cgaaaatggc agtattcggg ctgtgaccga
    52081 tagcaacaaa cagaccggca acgtcgagtg attcgatgtt atcgctgttt tgcgtatcgc
    52141 gcagacgaac gccagtgaca cccatttgat cgccggtcac ttcttccagc gtccggttgg
    52201 tgtgcagaat gatgttgccg ttctccactt tatccatcag acgcttaatg aggatttttt
    52261 ccgcgcggaa accgtcacgg cggtgaatca gatgtacttc cgaagcgatg ttagacagat
    52321 acaacgcctc ttcaaccgcg gtattgccgc cgccgatgac cgcaactttc tggttgcgat
    52381 agaagaaacc gtcgcaggtt gcacaagcag aaaccccacg gcctttaaag gcttcttcag
    52441 agggcaggcc gagatagcgt gcagaagctc cggtggcaat aatcagcgcg tcgcaagtgt
    52501 attcgccgtt atcgccattc agacggaacg gacggttttg cagatccacc ttgttgatat
    52561 gatcaaaaat gatttcagtt tcaaatttgg tggcatgttc gtgcatgcgc tccattaata
    52621 acggaccggt cagatcgttt ggatcgccag gccagttttc cacttctgtg gtggtggtca
    52681 gttggccgcc tttttccatg ccggtaatca gcacaggttg caggttggcg cgcgccgcat
    52741 agacagcagc ggtgtatccc gccgggcctg aacccaggat aagcagttta ctgtgtttgg
    52801 tcgtgcccat gagatcccca tagttgttgg cagacaatgg gcaggattgt agggaattta
    52861 ctgacgtaaa aaaagagtat gacgattttg ttaacaattt gtgcaatcgg cagcatcgat
    52921 aagcaggtca aattctcccg tcattatcac ctctgctact taaatttccc gctttataag
    52981 ctgattaaat gatgaataaa cgcccctgtt aatgaatatc tggcatgttg tactaaaaat
    53041 cgatgttttg ctttgacaat cccctggtgt tttgcgaaaa cattcgagga agaaaaaaaa
    53101 cagtattctt atatgcgcat aaccatgcat gtaaatacca tgtttaccgt gctagtgaaa
    53161 tctacgtatg gcgtggacag acgccattcg tgatgtcgat agctgccaca aggcaacggt
    53221 cttctcaccg tagacccagg cattgcgcgc cgtgaatctt catgatttcg gtctatcgtg
    53281 acgggtagcg actctgaaca gtgatgtttc agggtcagac aggagtaggg aaggaataca
    53341 gagagacaat aataatggta gatagcaaga agcgccctgg caaagatctc gaccgtatcg
    53401 atcgtaacat tcttaatgag ttgcaaaagg atgggcgtat ttctaacgtc gagctttcta
    53461 aacgtgtggg actttcccca acgccgtgcc ttgagcgtgt gcgtcggctg gaaagacaag
    53521 ggtttattca gggctatacg gcgctgctta acccccatta tctggatgca tcacttctgg
    53581 tattcgttga gattactctg aatcgtggcg caccggatgt gtttgaacaa ttcaataccg
    53641 ctgtacaaaa acttgaagaa attcaggagt gtcatttagt atctggtgat ttcgactacc
    53701 tgttgaaaac acgcgtgccg gatatgtcag cttaccgtaa gttgctgggg gaaaccctgc
    53761 tgcgtctgcc tggcgtcaat gacacacgga catacgttgt tatggaagaa gtcaagcaga
    53821 gtaatcgtct ggttattaag acgcgctaac acggaacagg tgcaaaatcg gcgtattttg
    53881 attacactcc tgttaatcca tacagcaaca gtactggggt aacctggtac tgttgtccgt
    53941 tttagcatcg ggcaggaaaa gcctgtaacc tggagagcct ttcttgagcc aggaatacac
    54001 tgaagacaaa gaagtcacat tgacaaagtt aagtagcggc cgtcgccttc tggaagcgtt
    54061 gctgatcctt attgtcctgt ttgccgtctg gttgatggct gccttactaa gctttaaccc
    54121 ttcggacccc agctggtcgc aaacggcctg gcatgaacct atccataatt taggtgggat
    54181 gcccggtgcg tggctggcag atacgctgtt ctttattttt ggcgtgatgg cttacaccat
    54241 tcccgtcatt attgtcggcg gttgttggtt tgcctggcgt catcagtcca gtgacgaata
    54301 cattgattat tttgccgttt cgttacgcat cattggcgtt ttggcgctca tccttacctc
    54361 ctgtggtctg gcggcaatca acgctgacga tatctggtat tttgcctccg gtggcgtcat
    54421 tggcagctta ctaagcacta cgttacaacc actgctacac agtagcgggg gaactattgc
    54481 gctgctctgc gtttgggctg cgggcctgac gctgttcacc ggttggtcat gggtgaccat
    54541 tgctgaaaaa ctcggcggct ggattttaaa cattctcacc ttcgccagta atcgtacccg
    54601 tcgcgatgat acctgggtcg atgaagatga atatgaagat gacgaagagt atgaagatga
    54661 aaaccacggc aaacagcatg aatcacgccg tgcccgtatt cttcgcggcg cgctagcgcg
    54721 tcgtaaacgg ttggcggaaa aattcattaa tccgatgggg cggcaaacag acgctgcgtt
    54781 gttctccggt aagcggatgg atgatgaaga agagattatc tacactgcac gcggtgtggc
    54841 tgccgacccg gacgacgtcc tattttcggg caatcgtgca acgcagccag aatatgacga
    54901 atacgatcca ttattaaacg gtgcgccaat taccgaacct gttgctgtag cagctgctgc
    54961 taccacggcg acacaaagct gggctgcgcc ggttgaacct gtgacccaga cgccgcctgt
    55021 tacttctgtt gatgttccac cttcgcaacc tacagtagcc tggcagcctg taccgggtcc
    55081 acaaacggga gagccggtta ttgctcctgc accggaaggt tacccacagc agccacaata
    55141 tgcgcagcct gcagtgcaat ataatgagcc gctgcaacaa cctgtacagc cacagcagcc
    55201 gtattatgcg cctgcagctg aacaacctgt gcaacaacct tactacgctc ctgcggcgga
    55261 acaacctgtt cagcaaccgt attacgcaac tgcgccggaa cagcctgcgc aacagccgta
    55321 ttatgcccct gcgccagaac aaccggtggc aggtaacgcc tggcaagccg aagagcagca
    55381 atccactttt gctccacagt ctacatacca gactgagcaa acttatcagc agccagccgc
    55441 tcaggagccg ttgtaccaac agccgcaacc cgttgaacag cagcctgttg tggagcctga
    55501 acccgttgta gaagagacaa aacccgcgcg tccgccgctt tactattttg aagaagtgga
    55561 agagaagcga gcccgtgaac gtgaacaact tgcggcctgg tatcaaccga ttccagaacc
    55621 ggttaaagaa ccagaaccga tcaaatcttc gttgaaagcg ccttctgctg cagcagtacc
    55681 tccagtagaa gccgctgccg ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact
    55741 ggcgacgggg gctgccgcaa ccgttgccgc gccagtcttc agtctggcaa atagcggtgg
    55801 accgcgtccg caagtcaaag agggaattgg cccgcagttg ccacgaccga aacgtattcg
    55861 cgtgccaact cgtcgtgaac tggcgtctta cggtattaag ctgccctcac agcgtgcggc
    55921 ggaagagaaa gcccgtgaag cccggcgcaa tcagtatgat tctggcgatc agtataacga
    55981 tgatgaaatc gatgcgatgc agcaggatga actggcacgt cagttcgccc agacacagca
    56041 gcaacgctat ggcgaacagt atcaacatga tgtgcccgta aacgcagaag atgcagatgc
    56101 tgcggcagag gctgaactgg ctcgtcagtt tgcgcaaact caacaacaac gttattccgg
    56161 cgaacaaccg gctggggcga atccgttctc gctggatgat tttgaatttt cgccaatgaa
    56221 agcgttgctg gatgatggtc cacacgaacc gttgtttacg ccaattgttg aacctgtaca
    56281 gcagccgcaa caactggttg caccgcagca gcagtatcag cagccacaac agcaggttgc
    56341 gccacagcag caatatcagc agccgcaaca gccggttgcg ccgcagccgc agtatcagca
    56401 gccacaacag caggttgcgc cgcagccaca atatcagcag ccgcaacaac cggttgcacc
    56461 gcagcagcag gataccctgc ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt
    56521 gcataaaccg acgacgccgc tgccttctct ggatttgctg acaccgccgc cgagcgaagt
    56581 ggagccggta gatacctttg cgcttgaaca aatggctcgc ctggtggaag cgcgtctggc
    56641 tgatttccgt attaaagccg atgtcgtcaa ttactctccg gggccggtta tcactcgctt
    56701 tgaacttaac ctggctcctg gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct
    56761 tgcccgttca ctttcgacgg tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta
    56821 tgtaggtctg gagttaccaa ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga
    56881 taacgccaaa ttccgcgata atccgtcgcc attaaccgtg gtgctgggta aagatatcgc
    56941 cggtgagccg gtggttgccg atctggcgaa aatgccgcac ttgttggttg cggggactac
    57001 cggttccggt aaatctgtcg gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca
    57061 gccagaagat gtgcgtttca tcatgatcga cccgaaaatg ctggagcttt cggtttatga
    57121 aggcattccg catctgttaa cggaagtcgt tactgatatg aaagatgccg ccaacgcgct
    57181 gcgctggtgt gttaacgaga tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg
    57241 taatctggcg ggttataacg aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc
    57301 agacccgtac tggaagccgg gtgacagtat ggatgcccag catccggtgc tgaaaaaaga
    57361 accatacatt gtggtgttgg ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa
    57421 agtggaagag ctgatagcac gtctggcgca aaaagcccgt gccgcgggta tccacctcgt
    57481 actggcaact cagcgtccat cggttgatgt tattactggt ctgattaaag cgaatattcc
    57541 gacccgtatc gcctttaccg tatccagtaa gattgactca cgtaccattc ttgatcaggc
    57601 tggcgcggaa tcactgctgg ggatggggga tatgctctac tctgggccga actccacgtt
    57661 gccggtacgt gtccatggtg cttttgttcg cgatcaggaa gttcatgccg tggtgcagga
    57721 ctggaaagcg cgtggtcgcc cacagtatgt tgatggcatc acttccgaca gtgaaagcga
    57781 aggtggcgcg ggcggtttcg atggcgctga agaactggat ccgttgttcg atcaggcggt
    57841 gcagtttgtc actgaaaaac gcaaagcgtc aatttctggc gtacagcgtc agttccgcat
    57901 tggttataac cgtgcagcgc gtattatcga acagatggaa gcgcagggga ttgtcagcga
    57961 acaggggcac aacggtaatc gtgaagtgct ggccccaccg ccgtttgact aattaatgca
    58021 ttgccggata aggcgcggta gcgtcgcatc cggcactcta tcaactgaaa attcagtatt
    58081 ttcttttttc ctcaagctga ttattagcct ggaatagaga gtagagggaa ctcccgatcg
    58141 ggagtgacgt aatttgagga ataatgatga aaaaaattgc catcacctgt gcattactct
    58201 caagcttagt agcaagcagc gtttgggctg atgccgcaag cgatctgaaa agccgcctgg
    58261 ataaagtcag cagcttccac gccagcttca cacaaaaagt gactgacggt agcggtgcgg
    58321 cggtgcagga aggtcagggc gatctgtggg tgaaacgtcc aaacttattc aactggcata
    58381 tgacacaacc tgatgaaagc attctggttt ctgacggtaa aacactgtgg ttctataacc
    58441 cgttcgttga gcaagctacg gcaacttggc tgaaagatgc caccggtaat acgccgttta
    58501 tgctgattgc ccgcaaccag tccagcgact ggcagcagta caatatcaaa cagaatggcg
    58561 atgactttgt cctgacgccg aaagccagca atggcaatct gaagcagttc accattaacg
    58621 tgggacgtga tggcacaatc catcagttta gcgcggtgga gcaggacgat cagcgcagca
    58681 gttatcaact gaaatcccag caaaatgggg ctgtggatgc agcgaaattt accttcaccc
    58741 cgccgcaagg cgtcacggta gatgatcaac gtaagtagag gcacctgagt gagcaatctg
    58801 tcgctcgatt tttcggataa tacttttcaa cctctggccg cgcgtatgcg gccagaaaat
    58861 ttagcacagt atatcggcca gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct
    58921 atcgaagccg ggcatctgca ttctatgatc ctctgggggc caccgggtac cggcaaaaca
    58981 actctcgctg aagtgattgc ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc
    59041 acctctggcg tgaaagagat tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca
    59101 ggtcgccgca ctattctttt tgttgacgaa gttcaccgtt tcaacaaaag ccagcaggat
    59161 gcatttctgc cacatattga agacggcacc atcactttta ttggcgcaac cactgaaaac
    59221 ccgtcgtttg agcttaattc ggcactgctt tcccgtgccc gtgtctatct gttgaaatcc
    59281 ctgagtacag aggatattga gcaagtacta actcaggcga tggaagacaa aacccgtggc
    59341 tatggtggtc aggatattgt tctgccagat gaaacacgac gcgccattgc tgaactggtg
    59401 aatggcgacg cgcgccgggc gttaaatacg ctggaaatga tggcggatat ggccgaagtc
    59461 gatgatagca gtaagcgggt cctgaagcct gaattactga ccgaaatcgc aggtgaacgt
    59521 agtgcccgct ttgataacaa aggtgatcgc ttttacgatc tcatttccgc actgcataaa
    59581 tcggtacgtg gtagcgcacc cgatgcggcg ctgtactggt atgcgcgaat tattactgcc
    59641 ggtggcgatc cgttatatgt tgcgcgtcgc tgtctggcga ttgcgtctga agatgtgggt
    59701 aatgctgatc cacgagcgat gcaggtggca attgcagcct gggattgctt tactcgcgtt
    59761 ggcccggcgg aaggtgaacg cgccattgct caggcgattg tttacctggc ctgcgcgcca
    59821 aaaagcaacg ctgtctacac tgcgtttaaa gctgcgctgg cggatgctcg cgaacgtccg
    59881 gattatgacg tgccggttca tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc
    59941 tacgggcagg aatatcgtta cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt
    60001 tacttcccgc gggaaatagc acaaacacgc tattatttcc cgacaaacag gggccttgaa
    60061 ggcaagattg gcgaaaagct cgcctggctg gctgaacagg atcaaaatag ccccataaaa
    60121 cgctaccgtt aatgttatcg ttgcggtaat gttgttactg tatccctgtg gtcgcaggct
    60181 gtggccacat ctcccattta attcgataag cacaggataa gcatgctcga tcccaatctg
    60241 ctgcgtaatg agccagacgc agtcgctgaa aaactggcac gccggggctt taagctggat
    60301 gtagataagc tgggcgctct cgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac
    60361 ctgcaagcgg agcgtaactc ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa
    60421 gatatcgagc ctttacgtct ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa
    60481 gccgagctgg atgctttaca ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg
    60541 cctgcagatg aagtgccggt aggtaaagac gaaaatgaca acgttgaagt cagccgctgg
    60601 ggtaccccgc gtgagtttga ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac
    60661 tctggcctcg actttgcagc tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa
    60721 gggcagattg ctcgcatgca ccgcgcactg tcgcagttta tgctggatct gcataccgaa
    60781 cagcatggct acagtgagaa ctatgttccg tacctggtta accaggacac gctgtacggt
    60841 acgggtcagc tgccgaaatt tgctggcgat ctgttccata ctcgtccgct ggaagaagaa
    60901 gcagacacca gtaactatgc gctgatccca acggcagaag ttccgctgac taacctggtg
    60961 cgcggtgaaa tcatcgatga agatgatctg ccaattaaga tgaccgccca caccccatgc
    61021 ttccgttctg aagccggttc atatggtcgt gacacccgtg gtctgatccg tatgcaccag
    61081 ttcgacaaag ttgaaatggt gcagatcgtg cgcccggaag actcaatggc ggcgctggaa
    61141 gagatgactg gtcatgcaga aaaagtcctg cagctgctgg gcctgccgta ccgtaaaatc
    61201 atcctttgta ccggcgacat gggctttggc gcttgcaaaa cctatgacct ggaagtatgg
    61261 atcccggcac agaacaccta ccgtgagatc tcttcctgct ccaacgtttg ggatttccag
    61321 gcacgtcgta tgcaggcacg ttgccgcagc aagtcggaca agaaaacccg tctggttcat
    61381 accctgaacg gttctggtct ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat
    61441 cagcaggctg atggtcgtat tgaagtacca gaagttctgc gtccgtatat gaacggactg
    61501 gaatatattg gctaataccc aatttttctg aatctaaaaa gcgcctgcgg gcgctttttt
    61561 tgtctccctt tgataccgaa caataattac tcctcactta cacgtaatac tactttcgag
    61621 tgaaaatcta cctatctctt tgattttcaa attattctat gtatacaagc ctatatagcg
    61681 agctgctata gaaataatta cacaatacgg tttgttactg gaatcaatcg tgagcaagct
    61741 tgagtgagcc attatgaaaa cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg
    61801 ccgtggtttg gtaaaaacga cagcgatcgg cggcctggca atggccagca gcgcattaac
    61861 attacctttt agtcggattg cgcacgctgt cgatagcgcc attccaacaa aatcagacga
    61921 aaaggttatc tggagcgcct gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca
    61981 cgtcgtggac ggtgaaatca aatatgtcga aacggacaat accggcgatg acaattacga
    62041 cggcctgcac taggttcgcg cctgcctgcg tgggcgttcc atgcgtcgcc gtgtctacaa
    62101 tccggatcgc ctgaaatatc cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga
    62161 gcgcattagc tgggaagaag cctacgacat catcgcgacc aatatgcagc gcctgatcaa
    62221 agagtacggc aacgagtcta tctatctgaa ctatggcacc ggtacgctgg gcggcaccat
    62281 gacccgctcc tggccgccgg gaaataccct ggtcgcgcgg ctgatgaact gctgcggcgg
    62341 ctatctgaac cattacggcg actactcctc cgcgcaaatt gcggaaggtt tgaactatac
    62401 ctacggcggc tgggcagatg gcaacagccc gtcggatatc gaaaacagta agctggtagt
    62461 gctgtttggt aataaccctg gcgaaacgcg aatgagtggc agtggggtga cttactatct
    62521 tgaacaggca cgccagaaat ctaatgcccg catgatcatc atcgatccgc gctataccga
    62581 caccggtgcc gggcgcgaag atgagtggat ccctattcgt ccgggaacag atgccgcact
    62641 ggttaacggt ctggcgtacg tcatgatcac tgaaaacctg gtggatcagg cattcctcga
    62701 taaatattgc gttggctacg atgagaaaac cctgccagcc agtgcgccga aaaatggcca
    62761 ctataaagct tatattctgg gtgaagggcc agatggcgtg gctaaaacac cggaatgggc
    62821 ctcacaaatc accggtgttc cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag
    62881 taccaaaccg gcgtttatca gccagggatg gggcccgcag cgtcacgcta acggtgaaat
    62941 cgcaacccgt gctatctcga tgctggcgat tctgaccggt aacgttggta ttaacggagg
    63001 caacagcggc gcgcgtgaag gttcatacag cttaccgttt gtccgtatgc cgaccttgga
    63061 aaacccgatc cagaccagca tttcgatgtt tatgtggacc gatgccattg aacgtggccc
    63121 ggaaatgacg gcgctgcgtg atggtgtacg cgggaaagat aagctggatg tgccgatcaa
    63181 aatgatctgg aactatgccg gtaactgcct gattaaccag cattctgaaa tcaaccgtac
    63241 ccatgaaatc cttcaggatg ataagaagtg cgagctgatt gtggttatcg actgccacat
    63301 gacctcatcg gcgaaatatg ctgacatcct gctgcctgac tgcaccgctt ccgaacagat
    63361 ggactttgcg ctggatgcat cctgcgggaa tatgtcttac gtgattttca acgatcaggt
    63421 gattaaaccg cgctttgaat gtaagaccat ctatgaaatg accagcgaac tggcaaaacg
    63481 tcttggcgtt gagcaacagt ttactgaagg ccgtacacag gaagagtgga tgcggcatct
    63541 gtatgcccag tcgcgggaag cgattcctga actaccaacg tttgaagagt tccgcaagca
    63601 ggggatcttt aaaaagcgcg acccacaagg gcaccacgtt gcttataaag ctttccgtga
    63661 agatcctcag gcaaattcac tgaccacgcc atcgggcaaa attgagattt attcgcaggc
    63721 gctggctgac attgccgcta cctgggaatt gccagaagga gatgtgatcg atccactgcc
    63781 gatctacacg ccgggctttg aaagttatca ggatccgttg aacaaacagt atccgctgca
    63841 gcttacgggt ttccactata aatctcgcgt tcactcaact tacggcgacg ttgatgtgct
    63901 gaaagcagct tgccgtcagg aaatgtggat caacccgctt gatgcgcaaa aacgcggtat
    63961 caacaacggc gataaagtca ggatctttaa cgatcttggt gaggttcata ttgaggcgaa
    64021 agtgacgcca cgaatgatgc cgggtgtggt cgcactgggt gaaggtgcct ggtatgaccc
    64081 ggatacaaaa cgtgtcgata agggcggttg tatcaacgta ctgaccactc aacgtccgtc
    64141 tcctctcgct aaggggaatc cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg
    64201 agtaaccgat gacaacccag tatggatttt ttattgattc cagccgttgc accggttgca
    64261 aaacctgcga gctggcctgt aaagactaca aagatttgac gccagaagtc agcttccgcc
    64321 gcatttatga atatgctggc ggcgactggc aggaagataa cggtgtctgg caccagaacg
    64381 tgtttgccta ctatctgtcg atttcatgta accactgcga agatccggct tgtactaaag
    64441 tctgcccgag cggtgcgatg cataaacgtg aagatggttt tgttgtggtc gatgaagatg
    64501 tgtgcattgg ctgccgctac tgccatatgg cttg
//
LOCUS       DAJTNN010000005        56411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTNN010000005 DAJTNN010000000
VERSION     DAJTNN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.005"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..940)
                     /gene="wcaC"
                     /locus_tag="OO150_000299"
     CDS             complement(<1..940)
                     /gene="wcaC"
                     /locus_tag="OO150_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5514570.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFS"
     gene            complement(937..1409)
                     /gene="wcaB"
                     /locus_tag="OO150_000300"
                     /pseudo
     CDS             complement(937..1409)
                     /gene="wcaB"
                     /locus_tag="OO150_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
     gene            complement(1412..2251)
                     /gene="wcaA"
                     /locus_tag="OO150_000301"
     CDS             complement(1412..2251)
                     /gene="wcaA"
                     /locus_tag="OO150_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR5514571.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGH"
     gene            complement(2429..4591)
                     /gene="wzc"
                     /locus_tag="OO150_000302"
                     /pseudo
     CDS             complement(2429..4591)
                     /gene="wzc"
                     /locus_tag="OO150_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
     gene            complement(4594..5037)
                     /gene="wzb"
                     /locus_tag="OO150_000303"
     CDS             complement(4594..5037)
                     /gene="wzb"
                     /locus_tag="OO150_000303"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5514572.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSWETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5043..6194)
                     /gene="wza"
                     /locus_tag="OO150_000304"
     CDS             complement(5043..6194)
                     /gene="wza"
                     /locus_tag="OO150_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5514573.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKTYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQVLMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVM
                     ATEFRLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            6503..6652
                     /locus_tag="OO150_000305"
     CDS             6503..6652
                     /locus_tag="OO150_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514574.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            6853..8436
                     /locus_tag="OO150_000306"
     CDS             6853..8436
                     /locus_tag="OO150_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5514575.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(8710..10563)
                     /gene="asmA"
                     /locus_tag="OO150_000307"
     CDS             complement(8710..10563)
                     /gene="asmA"
                     /locus_tag="OO150_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5514576.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRNYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEVVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(10585..11166)
                     /gene="dcd"
                     /locus_tag="OO150_000308"
     CDS             complement(10585..11166)
                     /gene="dcd"
                     /locus_tag="OO150_000308"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5514577.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(11258..11899)
                     /gene="udk"
                     /locus_tag="OO150_000309"
     CDS             complement(11258..11899)
                     /gene="udk"
                     /locus_tag="OO150_000309"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5514578.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHNLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            12217..15514
                     /locus_tag="OO150_000310"
                     /pseudo
     CDS             12217..15514
                     /locus_tag="OO150_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(15553..16410)
                     /gene="alkA"
                     /locus_tag="OO150_000311"
     CDS             complement(15553..16410)
                     /gene="alkA"
                     /locus_tag="OO150_000311"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5514579.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            16544..17896
                     /gene="yegD"
                     /locus_tag="OO150_000312"
     CDS             16544..17896
                     /gene="yegD"
                     /locus_tag="OO150_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5514580.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSNAAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(17908..19830)
                     /gene="yegI"
                     /locus_tag="OO150_000313"
     CDS             complement(17908..19830)
                     /gene="yegI"
                     /locus_tag="OO150_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR5514581.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPASKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINVNGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALYDTARERQKQ
                     KFLEGFFASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKASC
                     DRRFVFRPNEAVTPADRQAVMAAKRHRLESALTVGATELQRFRLYAPARTMPLMEPLR
                     QAAEKLAQAQADLSRC"
     gene            complement(19827..20588)
                     /gene="pphC"
                     /locus_tag="OO150_000314"
     CDS             complement(19827..20588)
                     /gene="pphC"
                     /locus_tag="OO150_000314"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCR5514582.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASVTQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(20585..21244)
                     /locus_tag="OO150_000315"
     CDS             complement(20585..21244)
                     /locus_tag="OO150_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5514583.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(21465..21524)
                     /gene="ibsA"
                     /locus_tag="OO150_000316"
     CDS             complement(21465..21524)
                     /gene="ibsA"
                     /locus_tag="OO150_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR5514584.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(21797..21853)
                     /locus_tag="OO150_000317"
     CDS             complement(21797..21853)
                     /locus_tag="OO150_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5514585.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(22125..22181)
                     /locus_tag="OO150_000318"
     CDS             complement(22125..22181)
                     /locus_tag="OO150_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5514586.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            22460..23707
                     /gene="mdtA"
                     /locus_tag="OO150_000319"
     CDS             22460..23707
                     /gene="mdtA"
                     /locus_tag="OO150_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR5514587.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGMIKVKARFNNQNDVLFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGNRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            23707..26829
                     /gene="mdtB"
                     /locus_tag="OO150_000320"
     CDS             23707..26829
                     /gene="mdtB"
                     /locus_tag="OO150_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR5514588.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLIFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYSAFGQRLISTIYTQANQYRVVLEHNTEITPGLATLDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            26830..29907
                     /gene="mdtC"
                     /locus_tag="OO150_000321"
     CDS             26830..29907
                     /gene="mdtC"
                     /locus_tag="OO150_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5514589.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGKLYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            29908..31324
                     /locus_tag="OO150_000322"
                     /pseudo
     CDS             29908..31324
                     /locus_tag="OO150_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            31321..32724
                     /gene="baeS"
                     /locus_tag="OO150_000323"
     CDS             31321..32724
                     /gene="baeS"
                     /locus_tag="OO150_000323"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5514590.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            32721..33443
                     /gene="baeR"
                     /locus_tag="OO150_000324"
     CDS             32721..33443
                     /gene="baeR"
                     /locus_tag="OO150_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5514591.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            33634..33966
                     /locus_tag="OO150_000325"
     CDS             33634..33966
                     /locus_tag="OO150_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5514592.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            34089..35489
                     /locus_tag="OO150_000326"
     CDS             34089..35489
                     /locus_tag="OO150_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCR5514593.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLRLAAMHILAKA
                     NPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLTFEEIRQLQENAPSVKHACAYC
                     RLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRNFNLSGIQPNALQRQQLQALFT
                     RNPELHNRLWGYIPFK"
     gene            35810..37171
                     /gene="yegQ"
                     /locus_tag="OO150_000327"
     CDS             35810..37171
                     /gene="yegQ"
                     /locus_tag="OO150_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5514594.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREYFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGVAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(37501..37818)
                     /locus_tag="OO150_000328"
     CDS             complement(37501..37818)
                     /locus_tag="OO150_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514595.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            38232..39130
                     /gene="yegS"
                     /locus_tag="OO150_000329"
                     /pseudo
     CDS             38232..39130
                     /gene="yegS"
                     /locus_tag="OO150_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
     gene            complement(39212..39985)
                     /locus_tag="OO150_000330"
     CDS             complement(39212..39985)
                     /locus_tag="OO150_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5514596.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(40091..41131)
                     /gene="gatD"
                     /locus_tag="OO150_000331"
     CDS             complement(40091..41131)
                     /gene="gatD"
                     /locus_tag="OO150_000331"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5514597.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITIGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGSMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(41179..42534)
                     /locus_tag="OO150_000332"
     CDS             complement(41179..42534)
                     /locus_tag="OO150_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5514598.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNIDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSVSLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFITWRRARGFIKQEKAVLA
                     E"
     gene            complement(42538..42822)
                     /gene="gatB"
                     /locus_tag="OO150_000333"
     CDS             complement(42538..42822)
                     /gene="gatB"
                     /locus_tag="OO150_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5514599.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(42853..43305)
                     /gene="gatA"
                     /locus_tag="OO150_000334"
     CDS             complement(42853..43305)
                     /gene="gatA"
                     /locus_tag="OO150_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5514600.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(43315..44577)
                     /gene="gatZ"
                     /locus_tag="OO150_000335"
     CDS             complement(43315..44577)
                     /gene="gatZ"
                     /locus_tag="OO150_000335"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5514601.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIVRGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(44606..45460)
                     /gene="gatY"
                     /locus_tag="OO150_000336"
     CDS             complement(44606..45460)
                     /gene="gatY"
                     /locus_tag="OO150_000336"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5514602.1"
                     /translation="MHVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATEPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(45768..46820)
                     /gene="fbaB"
                     /locus_tag="OO150_000337"
     CDS             complement(45768..46820)
                     /gene="fbaB"
                     /locus_tag="OO150_000337"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5514603.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVVSTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            47077..48354
                     /locus_tag="OO150_000338"
     CDS             47077..48354
                     /locus_tag="OO150_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5514604.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPGTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFCESDNEITAIKVDDRDIALTQGEVK"
     gene            48351..49355
                     /locus_tag="OO150_000339"
     CDS             48351..49355
                     /locus_tag="OO150_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR5514605.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSITRHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            49352..50317
                     /locus_tag="OO150_000340"
     CDS             49352..50317
                     /locus_tag="OO150_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5514606.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(50291..51037)
                     /locus_tag="OO150_000341"
     CDS             complement(50291..51037)
                     /locus_tag="OO150_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5514607.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(51089..51907)
                     /locus_tag="OO150_000342"
     CDS             complement(51089..51907)
                     /locus_tag="OO150_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5514608.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQKVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(51972..52772)
                     /gene="thiD"
                     /locus_tag="OO150_000343"
     CDS             complement(51972..52772)
                     /gene="thiD"
                     /locus_tag="OO150_000343"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5514609.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIESDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQYV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(52769..53557)
                     /gene="thiM"
                     /locus_tag="OO150_000344"
     CDS             complement(52769..53557)
                     /gene="thiM"
                     /locus_tag="OO150_000344"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5514610.1"
                     /translation="MQVDLQSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLPHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(53592..53688)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(55269..55562)
                     /locus_tag="OO150_000345"
     CDS             complement(55269..55562)
                     /locus_tag="OO150_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514611.1"
                     /translation="MYGQDTTAGGNLKKDSPLGKLWDFLHSPDLPTNPDFAKPADKTG
                     WVSSYSNPTYVPPTPTITAPQLKVDMAGNPLMTPPAWLNQPLNVNVNVKVQDG"
     gene            complement(55526..>56411)
                     /locus_tag="OO150_000346"
     CDS             complement(55526..>56411)
                     /locus_tag="OO150_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR5514612.1"
                     /translation="SEELKGQLSEALPGAVGLFVKALNQMKGTTNLTEKDLFDLMQQG
                     KLFSKDILPEVAKQMKAAARNGGALDKAMKSNRASWQRLQTSMQNAMNIFFASGFGNE
                     LTSAFDAISAAVNGSGGAFEMFGHIAGKIVEGATDAFTELHDTVILTFRIIEYYASKM
                     GITGEQLKAWGEMAAYAAGVAMFAGSIWKLGVAFKWLLTMLNPLTKLLGVMKGIAAIG
                     GIEAATTGGSGGEGKDKPNKPGRGPKVGKVGGAAALSGGPVGWSIAVGSVGGDYAWDK
                     VMTLTTPCMDRTPPPGAI"
BASE COUNT        13381 a        14814 c        14286 g        13930 t
ORIGIN      
        1 aactgaacac cagcgcatcc atctgattga gcgcgctcat caacttgcgc ttgtcagttt
       61 caaagccgtg attaaccacg ttgccagcgg tgaacggcga gaacttacca aaggtatgca
      121 gttcgatttt gtcgcccagc gccatcatct cacgcaccag ttgctggtta gtcttgccgt
      181 cgtaacgcaa gtcatgcgcc accaccgcga ttttcggctt gccctgggtt tcgcgcaccg
      241 gaggcaagtc cgccagaatc gcttcggttg ccatatcaat accattattg ataatccggc
      301 aacgccctgg accgtacagg ctattgaaag cgtcagccac atgctggctg ggggaaataa
      361 actgacagcc cagcgccagc atctcacgga ataactggcg tttgcccgcc accagctggt
      421 gtgcgcgatc aatcttcacc ggcggatagt tattcaaggt cgggcatttc tggcagcccg
      481 ttttccagcc ttcgcaaccg tcggtaaagg cgcagcgtcc ggtaacactc cagtggtcgt
      541 gcagcgtcca gaccagagtg acgtccggtt tatggttttt cactttttcg caaaagcgca
      601 ccacgctctt aagatttagc cagtagctgt gcagcacatg aaaatgcagg accaccggac
      661 ccggtgtgcg agtaatggtg cgatataact cattgaaatt gccaaacaga tcgcgattaa
      721 acagacgaaa cagggcaata ttcgccatcg cggtcatccg cggcgtatgt ttgatgacct
      781 gcggatagtt ctgatggctg acgctctctt tgccgccttt gccgtaaccg tagacaaaat
      841 gtgacgccag cccctgttgc agcgcacgct ggtggagatc taacgccaca cctgctgccc
      901 cgccttccgc cagtcgcaca ttaaattgca gaatattcat ttaattacct tcactcgcgc
      961 tttttctcct accaccagcg cgttgtccgg gacagagtcg agcaccacgc tgcccgcacc
     1021 cacggtgacg ttgttaccaa gcgtgatatc accaagaata atgacgttgg caccgagttc
     1081 gacgccgttg ccaatgtgtg gacatgccat gttatcggca ccacgattgc cgatagtgac
     1141 gccgtggcga atggtgaaat catcccccgc taccacgttt ttattgatca ctacggcgta
     1201 accgtgatgg atagtaaagc ggcggcctgg atttcataac cgaaaaagca ttcggtgata
     1261 atgcgataca gcaccagcag cggggccgcc cacagattgt tgaggacgtt ctttttgcgc
     1321 cacaccgaac aaaaatgagc aacacgatag gcaagaacca tgcagcacgg gcgtaaactc
     1381 cagctgttgg cacgcagatc ttccagcatc tttagtgccc ccgaatacca tcagccagac
     1441 gtttgccgtt acgcaccgac agcagcgtca gcaaagtgcg ccaggtcata cgtttattgc
     1501 ggatctggta gagggtaaac agctgatatt ttttgcttgc acggtcaaat ttatctttgt
     1561 gctttcggta aaagtggaag tacccggaga attttttcgg tgacgaggtg atctgcattt
     1621 caccgtgatt gatatgcagg atctgcgtcg cttcttccac tttccacggt tcgccgtact
     1681 ccaccaccat ccgcaggaag atgtcgtaat cctgtgctgc tttcagttcg gtatcgaaca
     1741 aacactcttt gaaacgccat gcccaagtaa agacctgatt gccaatgata ttgcgtttat
     1801 agaacaggcg gcgtgaatac ggcgatttgg gatatagcgg caaacttgct ggttgggaat
     1861 agacttcgcc ctggcaaacg tagtcgttag cgtacaaaaa ggcgtgagtg accagttgct
     1921 gtttatgggc gaggaagacg ctcagacggt tgggtgtcca ttcgtcatcg tcatcaatcc
     1981 cggtgatgta ttccccttgc gccagcataa tcgcctggtt acgtaccgcg cacgccccgc
     2041 tgttaatatc gttgtggatg taagtgatac gcggatcgtt gagggcggtg acgtactgtt
     2101 gcagttgttc ccaggaggtg gagcaatcat ccacgatgat catctcccag ttgctgtagt
     2161 cctggcgcag gaccgatttt atcgcccgaa tcgccagttg ttggcggttc caggtcggca
     2221 tatagattga gatcagcgga ttgtctttca taggttgttc tcccggagtg tcgagatgtt
     2281 ctgccggttt ataagcttgt ttacgggttc tgttgccgga tgcggcgtga gcaccttatc
     2341 cggcctacgg agctggtttg cttgccggat gcggcgtgaa ggtaggtcgg ataagatgcg
     2401 tcagcatcgc atccgacagc ggatgtcgtt atttcgcatc cgacttatat tcgtattcgt
     2461 aatagccata atcctgatac gcgctggcgc ggcggaagat ggagttcaga atcacccctt
     2521 tcaccggaat accgttttgc tcaaagcggc tcagactggt ttccacttct ttcaaggtgt
     2581 tgaccgcata acgcgccacc attaacgtgg ttccgacatg acgaccaaca attgccgcat
     2641 cggtcactgc cagaatcggc ggcgtatcaa tcaacaccag gtcatagttt ttactcgccc
     2701 agttcaccag ttcggcaaag cgttcgctca tcaacagttc agaaggattt ggcggcacct
     2761 gaccgcgcgg gatcaggtca aatttggcaa tagaggtcgg tttagcagcg gtagtaatat
     2821 cgccctgacc aatcagaatt tccgacaggc cattaacgtt attagtgcac agcagctcgt
     2881 gggtgtagcc tttgcgcata tcgcagtcga tcaacaacac ccgtttattg gtctggctga
     2941 tcaccgccgc caggttggcg cagacaaagg ttttaccgat tgacgggcta accccggtca
     3001 tcatcaacac attgttctgt gcctgcatca tcgcgaagtg caaactggta cgcagactgc
     3061 ggatggcttc aatcgccaga tcggttggat tccccatcgc cagtagctgg ctctgtttat
     3121 agcgtttaat ccctttgatg gttttgacgc tatcgcgcgc tttctgccat tccgacagcg
     3181 ggatgctggc atagacgctg ataccgtgtt cttccagcac ctgcgggctt tcgatgccgc
     3241 gattaaacaa cgagcgcagc agcacaccca cgatagaaag catcaggcca aggataatcg
     3301 cgccgaggat aatcagccct ttcttcggtt ttagcacgcc aggctgggtg attgccgggt
     3361 caacaatgcg cacatcgccg acggtgctgg cttcggtgat tttcagctcc tgctctttat
     3421 tcagcagttg catatagacc tgctgaccag actcgacatc gcgggtcagg cgcacaatct
     3481 cctgctgggt tttcggcatc gccgttacgc gaccgttaag tttggctttc tcttcttcca
     3541 gcgcctgacg tttctccagc agcgtgcggt acgccgggtg aactttggtg tacagcttgg
     3601 agatttccgc ctctttaaag gtcagttcgt tcaactgcgc gtcgatgttc accatcgaat
     3661 cgagcaccgc tttcgcttcc agcggcagat caacagaatc tttatcctga cggaaggcat
     3721 tcagtttgtt ttcggcaaca tcgaggcggc tacgtacttc cggtaactgt tgcgcgagga
     3781 aagcgaggct tttcgacgct tccgccgatt tgcgctcaat attttgttcc tgatagttac
     3841 gggcgatgct gttaagaatg tcgcggatct gttcgcgatc ttcaccggta taagtcaggc
     3901 tcagtacgcc tgcgtctttg ccgttctccg ttaccatcag gctgttttgc agttggttga
     3961 tcatccccaa cgtggagtat ttggtgacgg taaactcact gcccgggcgg gcgtgaatgg
     4021 cttcaaccat cagcgtgacg ccttcttttt tcagcatctg gcccgcttgc ccacgggcgc
     4081 taaagccgcc atcactgctc agggtgtagt ttttattgtc cagcacatta agcgtaaaca
     4141 cctagtccgc catctctttc gggcggttaa aggtagtcac tttcaccgtc tcgttctggc
     4201 gtcccatcag tcgatcccag cccgccccga aaatcgggaa cgtgtttttg ctcactgcaa
     4261 tatcgaggtc gagatcgtcc accgttttac caagcaccag acgcgaacga atcaactgga
     4321 tctcggcgtc cgatgcaggc ggtttgttgg ctaatgccga accaatgtcc tgcactaacg
     4381 aattgccgct gttttgctcg atttgtacca gtgcgtcggc actataaatc ggcgtggcga
     4441 agaaggtgta aaccacggca cagaggacaa atacagcggt gatgccaatc acccaccagc
     4501 gcgcttcaat gacggtgccg accaggcgac caatatcgat ttcatcactg cccgttaccg
     4561 gagcggcatg ttgttttact ttttctgtca ttcttatacc tgctctgcgt tcaatgcctg
     4621 cgcccactgg cgggcagacc gttcaagtaa tgtgtacacc gctgcaaacg tttcccagct
     4681 tttgcgatac ggatcgggga tttcacattc gttatcccag tgaccaaaca gcatcacttt
     4741 gccgcgcatc tcaggtgcca tctcgcataa gcgttcgata tggcgctttt ccatggtcaa
     4801 aatcaggtca tagttgcgac acaggcggcg gctgatttgt cgtgcacagt ggccttccag
     4861 agacagttga tgttctgcgg cgacgctgat agcggtagga tcagcgccct taccgaccag
     4921 cgcgccgagt ccagcggact ccactttcag ttccgggtga taacgttgca gtaagcgttc
     4981 cgccgtcggg gaacggcaaa tattgccgac acagacaact aatatgttgt taaacatgac
     5041 gattaccagt tatgaatgtc gctggctgta tccgtcatgt aacggacacc gctaatagtt
     5101 ggcagcaact gattgatcag acggttccag cgggaaaccg gggcggtggt gacatacacc
     5161 acgtcataag gttgcaggcg gaattctgtc gccatcacca gcgacgtggc atcggacata
     5221 tccagctggt agatattggc aatcttgccg ttacgcccgc cctcgccttt cagcggacga
     5281 atgacaaaga tgccgctggc gttggaggtg gtcatgtcga taccttcagc attacccagg
     5341 gcttcagtca gggcttcagt cagggtcatg ccgctaaagt ccattttcag ggtgctctgt
     5401 ttcttcactt cacccatcac aaatactttc agatcatcat tacgcggcac gtagagaata
     5461 tcgccgggat aaagcagtcg gttctgattg aggtcgccgt tttgcatcag cacctgcaaa
     5521 gaaatgcgtt cttcacgacc attgtgtgtt agcaccacgt tgcgccagtc agcggtgtcg
     5581 gtcaggccac ctgcggcgtt gatggcgtcg agaatggtca gtggtacgtt ggtgatcgcc
     5641 tgttgaccgg atttattcac ctgacctgag atataggtct tttgtgagcg gaaggcggcg
     5701 atattaacgt ccacctgcgg gtcagcgatg tacgtcgcta agcgcccggt aatatcactg
     5761 cggatttcag cgagcgtttt cccgactacg tgaaccttgc cgatatacgg gtaaaacata
     5821 gtgccgtcag gctgtaccca gttgccggtg tcgctggagc tgcggtactg accagctggc
     5881 gtggtgagtt ccgggtgatc ccagacggtg acattaagaa cgtcccccgg cccgacgcga
     5941 tactggtaat tcgcgatctc gctttccagc gtcatattgg ggcgcgctac attcgggcgt
     6001 gggcgtaatt ggtcaatcag gcgcggggtc agcggataaa cattcaccat tttgtcgaga
     6061 tcgaaatcag cgtcctgctg tttgatgacg tctttgccca tcgtcgacat attgctgccc
     6121 ggaagtactg tgcaaccgct tatcaaggtt actgacacca ataatggcat caatttcatt
     6181 ttggatttca tcattgttta tttatcactt tggcagagta attatcctgt gcactattaa
     6241 tagcaatgtc gccatgcaca tttaccttgc agttaattga atacaaattt aactggcatc
     6301 agtcctaaaa aaattgattt catccgcagg ctattgacag aataattcag actggtcttt
     6361 caggcatcca gacacgctac cgcccctggc tttttagcca ccaatacact gatttagctt
     6421 aattttttaa accctctctg catacaatcg ttgatgagaa agggttattg cggaaattaa
     6481 cttccgaata taaggtgaca ttatggtaat tgaatattgg ctctccaata atgcaggagg
     6541 aagtgttaca gctaacggaa tagcaggcaa ggtaacaatt cggcaattgg ctatttttaa
     6601 gaattatatt aagtgtcatt caatatggtt tttaggagtt tctttaggtt gacaatattt
     6661 aatattgcgc ctccgcatgc gatatttctt aaataatgtt ttattattac cacttttaat
     6721 tcagggataa tgtgaggtta ttaccctcaa ataatatgag ataaatagtg ctgcaacatt
     6781 gcatttttgc cccgatttat ccatgatcga attgtgacat ttgtcataca acgaataggt
     6841 tttgtactta ctatggaatg gattgccgat ccgtctatct gggccgggtt aatcacgctg
     6901 attgtgatcg aactggtcct cggcattgat aacctggtct ttattgccat cctcgccgaa
     6961 aaactaccgc cgaagcagcg cgaccgcgca cgggttaccg ggctgttgct ggcgatgtta
     7021 atgcgcctgt tactgctggc gtcaatctcc tggctggtca ccctgacgca accgctgttc
     7081 agcttccgct cgtttacctt tagcgcccgc gacttaatca tgctgtttgg tggtttcttc
     7141 ctgttgttca aagccacgat ggagctgaac gaacggctgg aagggaaaga cagcaataat
     7201 cccacacaac gcaaaggtgc gaagttctgg ggggtggtga cacaaattgt ggtactggac
     7261 gccatcttct cactcgactc agtgattacc gccgtcggga tggtcgacca tttactggtc
     7321 atgatggccg ccgtggttat cgctatcagc ctgatgttga tggccagcaa gccgttaacg
     7381 caatttgtta acagtcaccc gaccatcgtt attctctgct taagcttcct gttgatgatt
     7441 ggctttagcc tggtggcaga aggtttcggc ttcgtcattc cgaaaggcta cctgtacgct
     7501 gccattggtt tctcggtaat gatcgaggcg ctcaatcagt tggctatctt taaccggcga
     7561 cgttttcttt ccgctaacca gacgctacgc cagcggacta ccgaagcggt aatgcgcctg
     7621 cttagcgggc aaaaagaaga tgcggaactg gacgccgaaa ccgcgtccat gctggtggat
     7681 catggtaacc agcagatctt taatccgcag gaacggcgga tgattgagcg ggtacttaat
     7741 cttaaccagc gtactgtcag cagcattatg acgtcgcgcc acgatattga gcatatcgat
     7801 ctcaacgcgc cggaagacga gatccgccag ttgctggagc gaaatcagca tacgcggctg
     7861 gtggttaccg acggtgatga cgcagaagat ttgctcggtg ttgttcacgt tatcgacctg
     7921 ctacaacagt cactgcgtgg cgaaccgctc aacctgcggg tgttgattcg ccagccgcta
     7981 gtgttcccgg aaaccttgcc gttgttacct gccctggagc agttccgtaa tgcccgcaca
     8041 cactttgctt ttgtggtgga tgagtttggc tcggtggaag ggattgtgac attaagtgac
     8101 gtcactgaaa ccattgccgg taacttaccg aacgaagtgg aagagatcga cgcccgccat
     8161 gatattcaga agaatgcgga cggttcctgg acggcgaatg gtcatatgcc gctggaagat
     8221 ctggtgcaat atgtgccgct gccgctggat gaaaaacgtg aatatcacac cattgccggg
     8281 ctgttgatgg aatatttaca gcgtattcca aaacctggcg aagaagttca ggtgggggat
     8341 tatttgctta aaacgttgca ggtggaaagc catcgcgtgc agaaggtgca gattattccg
     8401 ctgcataagg atggcgagtt ggagtacgag gtgtgatggt atcggtcatt ttacccaata
     8461 gccgaaagga ataagatgag ggaaccgcgc cagcggcgat ttaccgccgg gagcccgggt
     8521 tgccagggtg gtggcgattg agcccccctg gcacgttcac cggcttgagc gttccatagt
     8581 agcgaggaac atcaagtgaa cggaacagcc ttcgatctta catattcaga atataatgat
     8641 gccgccacga acgcctgatg cgacgcttta cgcgtcttat caggcctaca ggacaggtca
     8701 ccaggtaacc tacatcttct ccagcaactt cttcacatct ttgccattgc gggaatcttt
     8761 attccgctcg gcccagtcat tcagacgacg tttcgcttcg tcctgtagat gtttgcgcag
     8821 taactgatcc acttgcagac tgtaattgag ttgctgccag ttgccataaa cccgcagcgg
     8881 tactggcgtt tctttaagga aatcaatcag tttgctttcc ccgttccagc cacccacgac
     8941 ccgaatatca aactgggtgt cgcaggtttg ctccgccaga ttcaacgtac cagccccgga
     9001 aagcgccagc acaggcgatt gaccttgcat gtcgtttaac gtcacgacgc catccttcaa
     9061 cgtcaaatcg gtggtaaagc ggtcaagacg cgttacgtta tcgaagtttt cagcggcctt
     9121 cacatcacca ccattacgtt ctaccgcttg ctgaatcatc tgctggaagt tcatcccttc
     9181 catgcgcgtg tcggtcattt cgacatgtgc ctgtccttgc cagttgtggc ggaatgcgtc
     9241 ggcatctatg tcagcaccgg agaaatcacc agccagtgac atctttccgg tcaacgaaat
     9301 cggatagtta aacgccttca gaatggtgcc aatctcaacg ttttccagcc gtggctggaa
     9361 gtttatccgc ggatttattg atgtcgcgtc cagcgtgccc ggcagtgaaa cctgtccacc
     9421 gttaagcttg ccctgcagtt gagtaatttc cagcaaaccc gacttgttgg tcatttgcgt
     9481 ggcaacatct gtaaaattca ttccgcgcca gcgcacgtta ctggcctgca acaaaatatc
     9541 agccgtaaag ccttgcagtc cctgatatgc cggttcatca atacgcgaag aaatgaccgg
     9601 gcgcggcaac gttgattggc tctgcccctg ctgcgcggca ccgttttcac cattcgctgt
     9661 ttcattaagc gggatgaggt tgtcaagatt cagttgcggg aattgcagtc tcagctgcca
     9721 ttccggtttc tctgtcagcg tgacctgtgc ttgcccgctc agcgtactgt cattggcggt
     9781 caaactaatt tggttaaatg aaaggcgttt atgtgactcc tgccactggg cctggaaact
     9841 cccctgccca tgaatacctt gttttggtaa atctgcaccc tgcaactgcc agttaatttg
     9901 ttcaatagcc gccgttaaat catgcggata atcagaagca tctaccgtac cgttaaggga
     9961 tatcgtgaga tcgcgctgat cgcgattaac ccgcccggag aactcaaatg aaccacgatg
    10021 ttggggatct tgttccattt gcaggcgaat attgcggatt gtcacctgct cgtcatcttc
    10081 atgctggaac accagcacgc tgtccgccac cttaagactg gatatatcaa acgaccatcc
    10141 gcgatcgtct gacagatccg gcaaggtatt gtcgcgcggt gcaaccggag cgtcttcact
    10201 gcgcaccact tccgtctgcg gcgtcagttg gatcactgcc ccttttagca tcacctgctt
    10261 aacgctcagt tgatgactca gtagtggtaa aagcgccacg tccagacgca tgttgtcggc
    10321 gcgaaccagt ggctggctcg cgccctgggc ggtgagagac attcgcccag agaggatact
    10381 aagctgcggc cagacgtgcc aacgcagtgg cccgtcgagc tgcaattgat aaccgctacg
    10441 tgcagcaact tgcttgacca tatagttgcg gaaatcattc ggattcacca gcaacactaa
    10501 cgcagataac ccggccacca gcacgaccag gagtatcatc agcgtcgtca gaaatcgtct
    10561 catggtatcc tcaatgggcc tgaattagtc tttatcgatt cggctggcta ccgcgccctg
    10621 ctggttgcga tatttcgcat cttcacggcg gttgtaaggt cgcgccgccg ggccggaaag
    10681 cggctcaaag ctcagcgcac caattaacat gcccggacgc agcgccagcg gcagcttacc
    10741 ggagttgtag aactccagca caatgcaacc agaccagccc ggatcgatac ggtgcgcggt
    10801 gacgtgcacc atcagcccca gacgcgccaa agaggaacgc ccgtccagcc agcccaccag
    10861 atcggctggc agcgtcaccg actccagcgt caccgccagc gccagttctc ctgggtgaag
    10921 atagaacgcc tcgccctcgt cgagaacgat ctcatcgctc atcacgcggt caagcgcggc
    10981 gctcacttca tctttgggac cgctcagatc gataaacgct gccgtgtgac cacggaaggt
    11041 acgaaattta ttgcccaggc gtacatccac cgtcgcgccg ttaatacgct ccactggcgg
    11101 acgtgggttg atcgacaaac ggccttcatc aagccaggct tcaatatctc ggtcacacag
    11161 acgcatggca ttttctcctt tcgcgcatca ctcccttaac gccaattacg tcaagggcat
    11221 accaggttat cactgaacgg tacacaattt atcaagctta ttcaaagaac tgacttattt
    11281 tcgctttcaa tatatcgatc gcgatgcggt ttttcccgcc gcgcggcacg ataatgtccg
    11341 catattgttt agaaggctca atgaattgca ggaacatcgg gcgcacggtt ttttgatatt
    11401 gcgccatcac ggaatccatt gaacgcccac gctcgttaac gtcacgcttg atgcggcgca
    11461 tcaggcagat atccagcggg gtatcaacga aaatggagaa gttaagttcg tcacgcaaac
    11521 gcgcatccgt cagcaacaaa atgccttcga gaatgatgac cttcttcggc tcaaccgtca
    11581 ccgtttcttt catacgcgta tgttcaacat agctgtaaac cggcaggtca attgccgagc
    11641 cgcgtttcaa cgcttgtaaa tgctcaagca gcaggttgtg atccatcgcg ctgggatggt
    11701 cgtagttggt cttaacgcgt tcttccatcg acagatggct ttgatctttg taatagcagt
    11761 cttcgggaat tacgccgatg tgttcatcac cgacttgctc acgtaattca cgataaaggg
    11821 tactggcaat aagactcttg ccggaagccg atgcgccagc gataccgata atgacgcact
    11881 gatgagactg atcagtcata tatttagcga cctgattaac ctggatgtta ggaagggggc
    11941 gacgaagcgc caaacgcggc aattataggg atttcatccg cctgatacca gtcgaatagc
    12001 gttgccgcgc gctcagagtt aattgttgac aaagaattcc cggtggcaaa ttacgttgat
    12061 cagttttatg taaggtaaaa aatgttatac gcagttgcgc aaattatccg cctttacgtc
    12121 actttatgag caattcacat ataaaatgta aaacttttgt actagcataa acacagaaac
    12181 gaatactggc gacctggtct tgcggataaa gcggtaatga gcaaacaatc acagcatgta
    12241 ttaattgccc tgccccaccc actgcttcac ctggtcagtt taggtttagt ctcgtttatc
    12301 tttacccttt tctcgcttga gctttcgcag tttggcaccc aactcgcccc actgtggttc
    12361 ccgacgtcca tcatgatggt ggcgttttat cgccatgccg ggcgcatgtg gccgggaatt
    12421 gcgctgagct gctcgctggg aaatatcgcc gcatccatcc tgcttttttc caccagctcg
    12481 ctgaacatga cctggacgac catcaatatt gttgaagccg tggtcggggc agtgctgcta
    12541 cgtaaattgc tgccgtggta taaccccttg caaaatctgg ctgactggct gcgtctggca
    12601 ttcggcagcg ccattgttcc gcctctgtta gggggtgttc tggttgtcct gctgacgccc
    12661 ggagacgatc ctctcagggc atttttgata tgggtactgt cagaatccat cggcgctctg
    12721 gtactggtgc cgctgggatt gttatttaag ccacactatc tgctgcgcca tcgcaaccca
    12781 cggttacttt ttgagtcact gctcacatta gccatcacgc tgacgttaag ctggctttct
    12841 atgttgtacc tgccgtggcc ttttactttc attattgtgc tgttgatgtg gagcgccgtg
    12901 cgcctgccac gaatggaagc ctttttgatc ttccttacca cggtgatgat ggtgtcgctg
    12961 atgatggccg cggatccctc cctgcttgct acgccgcgta cgtacctgat gagccatatg
    13021 ccgtggctac tgtttttgct gatcctgctg cccgccaaca tcatgaccat ggtgatgtat
    13081 gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa cccgctttcg gaacgcgatg
    13141 gaatattccg ctatcggtat ggcgttagtg ggcaccgagg gacaatggct gcaaaccaac
    13201 aaagcgctct gccagtttct cgggtacagt caggaagagc tgcgcggact cacctttcag
    13261 caactgacct ggccggagga tctcaataaa gatctccaac aggttgaaaa gctgataagc
    13321 ggtgaaataa acacctattc aatggaaaaa cgctactaca accgtaatgg cgatgttgtc
    13381 tgggcgttgc ttgccgtctc actggtgcgc cacacggatg gcacgccgct ctattttatc
    13441 gctcagattg aagacattaa cgagctaaaa cgcaccgaac aggtgaatca gcaactgatg
    13501 gagcgcatca cgctggctaa cgaagcgggc gggattggca tctgggagtg ggagctgaag
    13561 ccgaatattt ttcgagctgt atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg
    13621 gtacgagtgc gtgctgccgg aagatcgcca gcatgccgaa aaagtgattc gtgattcgtt
    13681 gcaatcacgc tcgcccttta agctggaatt tcgcattacc gtaaaagacg gtattcgcca
    13741 tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac gcctgctcgg
    13801 cattaatatg gatatgaccg aggttaaaca gcttaacgag gcattgtttc aggaaaaaga
    13861 gcgcctgcac attacgctgg attccatcgg tgaagccgtg gtctgtattg atatggcgat
    13921 gaaaattacc tttatgaatc cagtggcgga gaagatgagc ggctggacgc aggaagaagc
    13981 attaggtgtt ccgctcctga cggtgttgca tattactttt ggcgacaacg gaccattaat
    14041 ggagaacatt tacagtgccg acacctcacg ttccgcgatt gaacaagatg tggtgttgca
    14101 ctgccggaac ggcggcagtt acgacgtgca ttacagtatt acgccgttaa gtactctgga
    14161 cggcagcaat ataggctcgg tactggtgat tcaggacgtc accgaatcac gcaaaatgct
    14221 gcgccagctg agctacagcg cctcccatga tgcactgacg catctcgcca atcgcgccag
    14281 ttttgagaag caactgcgta tcctgctgca aacggtaaac agtacacatc agcgacatgc
    14341 cctggtgttt atcgatcttg atcgctttaa agcggtgaat gacagcgccg ggcatgcggc
    14401 gggtgacgct ttactgcgcg aactggcgtc gttgatgctg agtatgctgc gctccagcga
    14461 cgtgctggcg cgactcggtg gtgatgaatt tggtctactg ttgccagact gtaatatcga
    14521 aagtgcgcgt tttatcgcta cacgcattat cagtgccgtg aatgattatc actttatatg
    14581 ggaaggccgt gtgcatcggg taggtgccag tgccgggatt accttgattg atgacaacaa
    14641 tcatcaggcg gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg
    14701 tggacggggc cgggtgacgg tttacgaacc acagcaagct gccgcacata gcgaacgggc
    14761 ggcgatgtcg cttgatgaac agtggcggat gattaaagag aatcagttga tgatgatcgc
    14821 ccacggtgtc gcttcgccgc ggatcccgga agcgcgtaat ttgtggctga tttcacttaa
    14881 gctctggagt tgcgaaggcg agattattga tgaacaaaaa tttcgtcgta gcttcagcga
    14941 tccggcactt agccatgctc ttgaccgacg ggtattccac gaatttttcc agcaggccgc
    15001 aaaagcggtt gccagtaaag gcataagcat cgccctcccc ctttccgttg ccggtttgag
    15061 tagcgccacg ctggtgaatg atctgcttga gcagctggga aatagccctc taccaccacg
    15121 gttattacat ctgattattc cggctgaagc gattttagat cacgcagaaa gcgtgcaaaa
    15181 actgcggctg gtgggatgtc ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt
    15241 caactcgctg aaagcgaata tggcagatta cctgctactt gatggtgagt tatgcgccaa
    15301 cgtgcagggt aatttgatgg atgagatgct gattacgatt attcaggggc acgctcagcg
    15361 actcgggatg aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg atacgctttc
    15421 tggcatcggc gtcgatctga tttatggtga tgtgattgcc gatgcccaac cgctggattt
    15481 gctggtgaat agcagttatt tcgcgattaa ctgagagagc gtttgcccat tgcctgatgc
    15541 gacgtatcag gcctacaatt cgtccgttcc gtctggttgc cagccttccg tataccagat
    15601 atgcaacagc gcataagaac gccagggctt ccagcgctcg gcataacggc ggatttgtgc
    15661 cggtgtcatc cccggaaatc gctgtttaat cagataatca tccggcagaa aaacatcttt
    15721 cgcctgccag ccacgcaaag cgaaataatt cgccgtccag cgcccgatac ccggaaaagt
    15781 ttgcagcgtt ttcatcgcct gctccacatc gcccggaatt gtcattggta agctgccctc
    15841 cagcgccgca tttgccagat gaatcagtgc ctctgcccgt ttcaacggca tgcctaacgc
    15901 ttttaatgcc tgcgggtcgg ctgctgccag ccgctgaggc gtggggaagc agatatactc
    15961 cggaaaatca tccagccgtt cgccataaag ctgtacaact ttggcagtca attttgccgc
    16021 catcgccacg ctcaccagtt ggcctaaaat cgcccgcaca ccctgctcaa aagcatcaat
    16081 acagccgggt aaacgcaatc cgggccgcgc cgcgcctaac ttgcccaacg caccgttaac
    16141 aatctgcggg ttacattgca gatcaaacag gcggctcatt ttcgccagac actcagcggc
    16201 aacgggttct aaacctgcac ttaaatttat gtgcagagta tggcgggcta tatccggaat
    16261 agcagtcacc acgccgcgat attcgcccac cgccagacta cgggcataat aatcgtcagc
    16321 gaccgtttcc acaccactca ctgcacgggc ggcgagaaat cccaacatcc acgaccagtc
    16381 atacggcggc tgccagttca gggtatacat cgcatctcct tattcatccg ctttcagcat
    16441 aaacgttatt cagacgctac gctttgcttt catattccgg ttgtcgcgac ggcaacattt
    16501 cgctaaagtc acgccccttc ttcaccggca tggggattat ttcgtgttta ttggttttga
    16561 ttacggtaca gcaaactgtt cagtggcggt catgcgtgac ggtaaaccgc acttgctaaa
    16621 aatggaaaac gacagcacgc tgctgccttc aatgctttgc gcgccaacgc gtgaagcggt
    16681 aagcgaatgg ctgtaccgcc atcatgacgt tccggcagac gacgatgaaa cgcaggcgtt
    16741 gttacgacgg gcgattcgtt ataaccgcga agaagatatc gatgttacag cgaaaagcgt
    16801 gcagttcggt ctttcctcac tggcacagta cattgatgat ccagaagaag tgtggtttgt
    16861 gaaatcacca aaatcgttcc tcggtgccag cggcttaaaa ccgcagcagg tagcgctgtt
    16921 tgaggatctg gtctgcgcaa tgatgttgca cattcgccag caggcgcagg cgcagctgcc
    16981 agaagcgatt actcaggcgg tgattggtcg cccgatcaac ttccaggggc tgggcggcga
    17041 tgaagcaaac gcccaggcgc aagggattct ggaacgcgcg gcgaagcgtg ccggattcag
    17101 ggacgtggta ttccagtatg agccggtcgc ggctgggctg gattacgaag ccaccttgca
    17161 ggaagaaaaa cgggtgctgg tggtggatat cggtggtggt acgactgact gttcattgct
    17221 gctgatggga ccgcagtggc gttcgcgtct cgatcgtgaa gccagcctgc tgggtcacag
    17281 tggttgtcgt attggcggta acgatctgga tatcgccctg gcgtttaaaa acctgatgcc
    17341 actgctgggt atgggtggcg aaaccgaaaa aggcatcgcc ctgccgatcc tgccgtggtg
    17401 gaatgcggtt gccatcaacg acgtacctgc gcagagtgat ttctacagta gtgccaacgg
    17461 tcgtctgctt aacgatctgg tacgcgatgc ccgcgaaccg gaaaaagtgg ccctgttaca
    17521 gaaagtctgg cgtcagcgtt taagctatcg cctggtacgc agcgcagaag agagcaaaat
    17581 cgccctttca aacgctgcgg aaacccgcgc ctcactgccg tttatcagcg atgaactggc
    17641 tacgctgatt agccagcaag ggctggaaag cgccctcagc cagccactgg cgcggattct
    17701 ggaacaggtg caactggcgc tggataacgc ccaggaaaaa ccggacgtta tatacctgac
    17761 cggcggtagc gcccgttcgc cgctgattaa aaaggctctg gcagaacagt tgccgggcat
    17821 tccgattgca ggcggcgatg actttggctc cgtcaccgcc gggctggcac gctgggcgga
    17881 agtggtattt cgttaaaaga aaatggctca gcagcggctt aaatctgcct gcgcctgagc
    17941 cagtttttct gccgcctgac gtaacggttc catcaacggc atggtccgtg ctggagcata
    18001 aaggcggaat cgctgcaact ctgtcgcgcc gacagtcaac gccgattcca gccgatgtcg
    18061 tttggcggcc attaccgcct gtctgtctgc tggcgttacc gcttcgttcg gcctgaacac
    18121 aaaacggcgg tcacagctcg ctttccagtc gataaccgcc tgggtcagat ggtcgccaaa
    18181 ccctttaact tgcttaacgc cacgacgggt aacatccgct gctgtttcaa taccaaaaga
    18241 ccgtaacgcc gctttacgcg cagggccaac gccgggaata gaggcaaaaa atccctccag
    18301 aaacttctgc ttctgccgtt cccttgcggt gtcataaagt gctgccagag cgcgtttctc
    18361 ctcttccggt aacccgagaa tttcgtcctt cattttttcg agcatcgccc gtttggcgat
    18421 aaaaccttcc agcccgccca actgttggat ctggctgact aaatgctcat agtccatttt
    18481 cgcgcgatta aatgcctcac ggcgttgctg gatttctgct ttgtacactt tgcttgtcag
    18541 actgccaata atccagatag ccgacagcac aggaaccaaa ataatataac gcggttctgc
    18601 ctgaaggccg caaagcaaca gcgataacgc tgacagtgcg atctcaatca gaatgatgta
    18661 ttcgcgccgt aacaggccta aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa
    18721 tggcaattgc aatgctggcg gtgctactga cgccatcacc atcgcccaga ctttcgccag
    18781 cacaaaatca ccgctggtgg taatgacctc ttcaccgaga tcaacaaaat agataacgcc
    18841 ttgattatcc agcgtacacc acgggcagtc ggtcaaatga gcgggataaa catgcattgc
    18901 cgaaacggga catttcttta actgttggcg cagagaatca agtgccgcta cccacgcttt
    18961 agccgtcgga cgcccggttg ccacgccact ttccgtgaat gcctgctgaa acatggcttc
    19021 aacatcactc ggtaacatcg atagcggaat cgatcgtggc ggcggtttta aaccacgtcg
    19081 ctgattatct gacgcgtagg cataacggaa atgggtaata tccgtctcca gcgcattacc
    19141 cgcatcagag ataagcggca caccagaata aggatgccgg ccaccaaaca agacgtgaaa
    19201 aatcagcaac gcaaggccaa aattatcgtg attttctgtg cgttcaaagc cgacaaatga
    19261 cgacaaggtt tgtagctctg gcggcgtaaa atgcgacacg ccgacttcgc ataaatgcag
    19321 tgtgccattg acgttaatct gaaaggagtc gctatcaatc agtaccactt tgctgtcgcg
    19381 acctaccata aaactgttct ggtttacgtc acccacgacg tgcccgtgct cgtgaaccgt
    19441 agcaaaagat gaagcaatat tgcgcgcaac atagagtaga aaatcccacg cacaatgagg
    19501 gtaactctgg cgacgatgtg ccgggctata gatcatatga atcggttctt taccagaaac
    19561 ttttggcatc ataaaaccga taacttttcc gcctcgtcca ccgtgaagcg ttgcctgcgg
    19621 ccaggcgaca taattcaaca actgcgcgtc agctgtcgca gccataaagg caagtttgtc
    19681 ctgttttgag gcgggtggcg gcgtgtgata aatcttggcg acgctatcga caaactcctc
    19741 gatatcataa accgcgcctt caccgccttt gcccagttca cggcctggcg taacgcactc
    19801 accagtagca gtatataaag tgggtttcat ggtaaccaca gcgccagtgc caacgtttta
    19861 tcatcatccg tacgctcgtt taccgccggg ctggacaaaa actgctttaa caattcaggt
    19921 aataaatcga gttgttcctg cgttactgac gccagtccat tgaaaaacgg ggtaaagaaa
    19981 ggcacatgag gagaattatc cagcatattt aacgccagcc gctgaatacc atccgtaaat
    20041 gctgcaactt tatgcgcacg cccagtgctg gtgaaagttt ccagtctgga aacggcatct
    20101 tcgtcggtaa tgaagtgcgt catgttggca tattcaccca ccatcggcgt gagaggcaat
    20161 tgcagaccgt gacccaaatc taccaccacg ccgccatcgc caatctgcat aatcaaggtg
    20221 ccgtcaggcg atgaaatcaa tcccagaaac gtgcaggcaa aatcgcgaac tgctaatgct
    20281 ttggcttctg cctcggcaaa aagtcgttga cgaatagtca gtaccatatt tgtggcgagg
    20341 acatcattaa gccccaattc accgtcctgt actttttgca ccataaacgc catcgcttca
    20401 ttgacggcga gcgtcgcgcc ttctcctccc tgcgaaacgc tacctgcgcc atcagcaagg
    20461 aacatcacca gtaatggttg ctggtcgttt aaccagacaa cctgcatctg gcaggcatcc
    20521 tgacaaggca aatcagcact aatgtgcgat gttccaacag cggatgcata aaccagacgc
    20581 cagctcacac tgacgtccag ccttttggcg cttccagcac aacttccgtt cccggcgtgg
    20641 agcgggaaac cgaacgcagc gaactggata accagctaaa cagttcacgg aattgtaatc
    20701 cctgcagtgg caaaggctga cgaacgctga tttgtgccag agtcttcata tccgccccct
    20761 gaacaccgat ggaaaagaag gcaaacttct tatcttcttc cccctgaaac actttgttgg
    20821 cggcagcctg ccactcatcg gttggagcgc catcggtgat caggaaaatc cacgggcgat
    20881 aatacgaaat accatttgcg cggtactcac gtttcctctc ttcaaccata tctagggctt
    20941 tggtaatggc ggcccccata ggcgtatccc cctgggcaaa caggatgggc gggaaaaagt
    21001 tagcggcgct ggtaaagggt tgttccacat gcaccgggcc gaacgtcaca atgccgagtt
    21061 ccactctttt taaagccagc ggatcagcaa gcagttcatc acgaaaggta accaatccgg
    21121 cgttaagttc attaataggt ctgccgctca tcgagccaga aacatccagc agcaaaatgc
    21181 aggggcaacg tggttccggg ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc
    21241 tcataagaaa tcctttctta gagaattatc gaggtccggt atcataccga ttgcaataat
    21301 gaaataagag caaacgaaga aactttcaga attaggcccg tcggcgcaga aaattttgct
    21361 attcgcctcg caagaacgga gagccatcga ttgacactaa gggcggagtg acataatttc
    21421 aggagtgagg gttagggaga ggtttccccc tccccctggt gttcttagta agcctggaag
    21481 ctaatcacta agagtatcac cagtatgatg acgtgcttca tcataaccct ttccttatta
    21541 aaagccctct tctccgggag aggctttccc gtttcagcgt cccgctgaaa tcatcggctt
    21601 acctcctttc gccctgaatg cagtctatcg ctaacgcgtg aataaaatag tttcctctgt
    21661 gattattgcg taacgactcg caaagaatat ttgctggcag gatcgcagac tacaaagcct
    21721 acggattgac aatcttatcg tgaaggcata ctttcaggag tgagggtaga gcggggtttc
    21781 ccccgccctg gtagtcttag taagcgggga agcttatgac taagagcacc acgatgatga
    21841 gtagcttcat catgaccctt tccttattta aggccccttc ctcgggaggg gctttcccgt
    21901 ttcagcgtcc cgctgaaatc gtcggcttac ctcctttcgc cctgtatgca gtctatcgct
    21961 gacttgcgga taaaatagtt tccagtgtga ttattgcgta acgtctcgca aagaatattt
    22021 gctggcagga tcgcagactg caaagcctgc agattgacaa tcttatcgtg aaggcatact
    22081 ttcaggagtg agggtagagc ggggtttccc ccgccctggt agtcttagta agctggccag
    22141 ctaatgacta agagcaccac gatgatgagt agcttcatca tgaccctttc cttatttatg
    22201 gccccttcct cgggaggggc tttcccgttt cagcgtcccg ctgaaatcgg cggcttacct
    22261 cctttcgcca tgcaagcagt ctatccctaa cgcgtagata aaatagtttc ctgtgttatt
    22321 actggatgcg tgctcgcaaa tgtgcccgtc attcagacaa ttcccgacag tgtttcataa
    22381 ttcctccatt tttctccctt attggctggc tacactagta tcattccgcg aaacgtttca
    22441 ggaagagaaa ctcttaacga tgaaaggcag ttataaatcc cgttgggtaa tcgtaatcgt
    22501 ggtggttatc gccgccatcg ccgcattctg gttctggcaa ggccgcaatg actcccggag
    22561 tgcagcccca ggggcgacga aacaagcgca gcaatcgcca gcgggtggtc gccgtggtat
    22621 gcgttccggc ccattagccc cggttcaggc ggcgaccgcc gtagaacagg cagttccgcg
    22681 ttacctcacc gggcttggca ccattatcgc cgctaatact gttacggtgc gcagccgcgt
    22741 ggacggtcaa ctgatggcgt tacatttcca ggaaggccag caggtcaaag caggcgattt
    22801 actggcagaa attgacccca gccagttcaa agttgcatta gcacaaaccc agggccaact
    22861 ggcaaaagat aaagccacgc ttgctaacgc ccgccgtgat ctggcgcgtt atcagcaact
    22921 ggcaaaaacc aatctcgtct cccgtcagga gctggatgcc caacaggcgc tggtcagtga
    22981 aaccgaaggc accattaagg ctgatgaagc aagcgtcgcc agcgcgcagt tgcaactcga
    23041 ctggagccgc atcaccgcac cagtcgatgg tcgcgttggt ctcaagcagg ttgatgttgg
    23101 taaccaaatc tccagtggtg ataccaccgg aattgtggtg atcacccaga cgcatcctat
    23161 cgatttagtc tttaccctgc cggaaagcga tatcgctact gtagtgcagg cgcaaaaagc
    23221 cggaaaaccg ctggtggtag aagcctggga tcgcaccaat tcgaagaaat taagtgaagg
    23281 cacgctgtta agcctcgata accaaatcga tgccacaacc ggtatgatta aagtgaaagc
    23341 acgctttaat aatcagaatg atgtgctgtt tcccaatcag tttgttaacg cgcgcatgtt
    23401 agtcgacacc gaacaaaacg ccgtagtgat cccaacagcc gccctgcaaa tgggcaatga
    23461 aggccatttt gtctgggtgc tgaatagcga aaacaaggtc agcaaacatc tggtgacgcc
    23521 gggcattcag gacagtcaga aagtggtgat ccgtgcaggt atttctgcgg gcaatcgcgt
    23581 ggtgacagac ggcattgatc gcctgaccga aggggcgaaa gtggaagtgg tggaagccca
    23641 gagcgccacc actccggaag agaaagccac cagccgcgaa tacgcgaaaa aaggagcacg
    23701 ctcctgatgc aggtgttacc cccgagcagc acaggcggcc cgtcgcgcct gtttattatg
    23761 cgtcctgtcg ccaccacgct gctgatggtg gcgatcttac tcgccgggat tatcggttat
    23821 cgcgccctgc ccgtttcggc gctgccggaa gtggactatc cgaccattca ggtggtcacg
    23881 ctctacccag gtgccagccc ggatgtcatg acctctgccg ttaccgcgcc gctcgaacgc
    23941 cagttcgggc agatgtctgg cctgaaacag atgtcgtcgc aaagttccgg cggtgcgtca
    24001 gttatcactt tgcagttcca gctaacatta ccgctcgatg tcgccgagca ggaagtgcag
    24061 gccgcgatta acgctgcgac caacttgttg ccgagcgatc tgcctaaccc gccggtttac
    24121 agcaaagtga acccggcaga tccgccgatc atgacgctcg ccgtcacctc aaccgccatg
    24181 ccgatgacgc aagtggaaga tatggtggaa acccgcgtcg cgcagaaaat ctcgcagatt
    24241 tccggcgtcg gcctggtgac gctttccggc ggtcagcgtc cggctgttcg cgtcaaactt
    24301 aacgctcagg cgattgccgc cctcggcctg accagcgaaa ccgtgcgcac cgccattacc
    24361 ggcgctaacg ttaactcggc aaaaggtagc ctcgacggcc cttcccgtgc ggtcacgctt
    24421 tccgcgaacg accagatgca atccgccgaa gagtatcgcc agctaatcat cgcctaccag
    24481 aacggcgcgc caattcgtct gggcgatgtc gcaactgtag agcaaggtgc agaaaacagc
    24541 tggctcggcg cgtgggcgaa caaagaacag gccattgtga tgaatgttca gcgccagccc
    24601 ggtgctaaca ttatctccac cgccgacagc attcggcaga tgctgccaca gctcactgag
    24661 agtctgccga aatcggtgaa ggtgacagtg ctttccgatc gcaccaccaa tatccgcgca
    24721 tccgtcgatg atactcagtt tgaattgatg atggctatcg cgctggtagt catgattatc
    24781 tacctgtttt tgcgcaatat tccggcgacc atcattcccg gtgttgctgt accgctgtcg
    24841 ttaatcggca ctttcgcggt tatggtgttt ctcgattttt caatcaataa cctgacactg
    24901 atggcgttaa ctatcgccac cggattcgtg gtcgatgacg ccatcgtggt gatcgaaaac
    24961 atttcccgct atatcgaaaa aggcgaaaaa ccgttggcgg cggcgctcaa gggcgcaggt
    25021 gaaatcggct ttaccattat ctcgctgatc ttctcactga ttgcggtgtt gatcccactg
    25081 ctgtttatgg gcgatatcgt cgggcgactg ttccgcgaat ttgctattac cctggcggta
    25141 gcgattttga tctcagcggt ggtgtcgctg accctgacac cgatgatgtg cgcgcggatg
    25201 ctcagccagg agtcgttgcg taaacagaac cgcttctccc gtgcctcgga aaaaatgttc
    25261 gacaggataa tcgccgccta tggtcgtgga ctggcgaaag tgctgaatca tccgtggctg
    25321 accttaagcg tggcactcag cacgctgctg cttagcgtgc tgctgtgggt gttcattccg
    25381 aaaggtttct tcccggtaca ggacaatggc attattcagg gcactttgca ggcaccgcaa
    25441 tccagctcct ttgccaatat ggcccagcga caacgccagg tcgcggacgt gattttgcag
    25501 gatccggcag tgcaaagcct gacctcattt gttggcgttg atggcactaa cccgtcgctg
    25561 aatagtgcac gtttacaaat caacctcaaa ccgttggatg aacgtgatga tcgggtgcaa
    25621 aaagtcatcg cccgtctgca aacggcggta gataaagtgc cgggcgtcga tctcttcctg
    25681 caaccaacgc aggatctgac tattgatact caggtcagcc gcacccagta ccagtttacc
    25741 ttgcaggcca cctcactgga tgcgctcagt acctgggtgc cacagttgat ggaaaaactc
    25801 cagcaactgc cacagctttc tgatgtctcc agcgactggc aggacaaagg gctggtggcg
    25861 tatgtcaatg ttgatcgcga cagcgccagc cgtctgggga tcagcatggc ggatgtcgat
    25921 aatgccctgt acagcgcgtt tggtcagcga ctgatttcca ctatttatac tcaggctaac
    25981 cagtatcgcg tagtgctgga gcacaacacc gaaattaccc cgggcctcgc gacgctggat
    26041 accattcgcc tgaccagtag cgacggcggc gtggtgccgt taagctcaat tgccaaagtt
    26101 gaacagcgtt ttgcgccgct ttccatcaac catctggatc agttcccggt aacgaccatc
    26161 tcctttaacg tgccggataa ctattcgctg ggcgatgcgg tgcaggcgat tatggacacc
    26221 gaaaagacgc tgaatctgcc ggtggatatc accacgcaat tccagggcag caccctcgcc
    26281 ttccagtcgg cgctgggcag cactgtctgg ctgattgtcg cggcggtggt ggcgatgtat
    26341 atcgtgctcg gcattctgta cgagagcttt attcacccga tcaccattct ctcgacgcta
    26401 cccaccgcag gggtcggcgc gctgctggca ttaatgattg ccggtagcga actggatgtg
    26461 attgcgatta tcggcattat tttgctgatc ggtattgtga agaagaacgc catcatgatg
    26521 atcgacttcg cgctggctgc tgagcgcgag caaggcatgt cgccgcgcga tgcaatctac
    26581 caggcttgtc tgttgcgttt tcgtccgatc ctgatgacca ccctggcggc tctgcttggc
    26641 gcgctgccgc tgatgttgag taccggggtc ggcgcggaac tgcgtcgtcc gttaggtatc
    26701 ggcatggtcg gcggtctgat tgtcagccag gtgctgacgc tgttcaccac gccggtgatt
    26761 tatctgctgt tcgaccgcct ggcattgtgg accaaaagtc gctttgctcg ccatgaagag
    26821 gaagcgtaag tgaagttttt tgccctcttc atttaccgtc cggtggcgac gattttactg
    26881 tcggttgcca ttaccctgtg cggcatactg ggcttccgta tgctgccggt cgccccgctg
    26941 ccgcaggtcg attttccggt gattatggtc agcgcctcgc tgcccggtgc gtcaccagaa
    27001 acaatggcgt cttccgttgc cacgccgctg gagcgctcac ttgggcgcat tgccggagtc
    27061 agtgaaatga cctccagcag ttcgctcggc agcacgcgta ttattttgca gtttgatttt
    27121 gaccgggata tcaacggcgc ggcgcgcgat gtgcaggcgg cgatcaacgc tgcacaaagt
    27181 ttgctgccca gcgggatgcc cagccgtccg acctatcgca aagctaaccc gtcggatgcg
    27241 ccaattatga tcctcacgct gacatccgat acttattcgc agggtaaact gtacgatttc
    27301 gcctcgacgc agctggctcc gacgatttcg caaatcgacg gtgttggtga tgtcgatgtc
    27361 ggcggcagct cactgcccgc cgtacgcgtc gggctgaatc cgcaggcgct gtttaatcag
    27421 ggcgtctcgc tggacgacgt acgcactgcc atcagcaatg ccaacgtgcg taaaccgcag
    27481 ggggcgctgg aagatggcac tcaccgctgg cagatccaga ccaatgatga gctaaaaacc
    27541 gccgccgaat atcagccgct gattattcat tacaacaacg gcggcgcggt tcgtctgggc
    27601 gatgtggcga cagtgaccga ctctgtacag gatgtacgta acgccgggat gaccaatgcc
    27661 aaaccggcta ttttgctgat gatccgcaag ctgccggaag ccaatattat ccagacggtt
    27721 gacagcatcc gggcaaaatt accggagttg caggaaacca ttccggcggc gattgatctg
    27781 caaattgctc aggatcgctc ccccaccatt cgcgcctcgc tggaagaagt cgagcaaacg
    27841 ctgattatct cggtggcgct ggtgattctg gtggtgtttt tattcctgcg ctcgggtcgc
    27901 gcaactatta ttcccgccgt tgcggtgccg gtttcgctga ttggtacgtt tgcggcgatg
    27961 tacctgtgcg gtttcagtct caataatctt tcgttaatgg cgctcaccat cgctactggt
    28021 ttcgtggtgg atgacgccat cgtggtgctg gaaaacattg cccgtcatct ggaagcggga
    28081 atgaaaccgt tgcaagccgc actgcaaggt actcgcgaag tcggttttac ggtgctgtcg
    28141 atgagtctgt cactggtggc ggtgttcctg ccgctgctgt tgatgggcgg attgccgggc
    28201 cgactgttac gcgaatttgc cgtgacgctt tctgtcgcca ttggtatatc gttgctggtt
    28261 tctctgacat taacgccaat gatgtgtggc tggatgctga aagccagcaa gccgcgcgag
    28321 caaaagcgac tgcgtggttt tggtcgcatg ttggtagccc tgcaacaagg ctacggcaag
    28381 tcactgaaat gggtgctcaa tcatacccgt ctggtgggcg tggtgctgct tggcaccatt
    28441 gcgctgaata tctggctgta tatctcgatc ccgaaaacct tcttcccgga gcaggacact
    28501 ggcgtgttga tgggcgggat tcaggcggat cagagtattt cgtttcaggc gatgcgcggt
    28561 aagttgcagg atttcatgaa aattatccgt gacgatccgg cagtggataa tgtcaccggc
    28621 tttacaggcg gttcgcgagt gaacagcggg atgatgttta tcaccctcaa gccacgcgac
    28681 gaacgcagcg aaacggcgca gcaaattatc gaccgtctgc gcgtaaaact ggcgaaagaa
    28741 ccgggggcga atctgttcct gatggcggta caggatattc gcgttggtgg gcgtcagtcg
    28801 aacgccagct accagtacac gttgttatcc gacgacctgg cggcactgcg agaatgggag
    28861 ccgaaaatcc gcaaaaaact ggcgacgttg ccggaactgg cggacgtgaa ctccgatcag
    28921 caggataacg gcgcggagat gaatctggtt tacgaccgcg acaccatggc acggctggga
    28981 atcgacgtac aagccgccaa cagtctgtta aataacgcct tcggtcagcg gcaaatctcg
    29041 accatttacc agccgatgaa ccagtataaa gtggtgatgg aagtggatcc gcgctatacc
    29101 caggacatca gtgcgctgga aaaaatgttc gttatcaata acgaaggcaa agcgatcccg
    29161 ctgtcgtatt tcgctaaatg gcaaccggcg aatgccccac tatcggtgaa tcatcaggga
    29221 ttatcggcgg cctcgaccat ttcgtttaac ctgccgaccg gaaaatcgct ctcggacgcc
    29281 agtgcggcga tcgatcgcgc aatgacccag cttggggtgc cttcgacggt gcgcggcagt
    29341 tttgccggca cggcgcaggt gttccaggag acgatgaact cgcaggtgat cctgattatc
    29401 gccgccatcg ccacggtgta tatcgtgctg ggtatccttt acgagagtta cgtacatccg
    29461 ctgacgattc tctccaccct gccctcggcg ggcgttggag cgctgttggc gctggagctg
    29521 ttcaatgccc cgttcagcct aatcgccctg atagggatca tgctattaat cggcatcgtg
    29581 aagaaaaacg ccattatgat ggtcgatttt gcgcttgaag cccaacggca cggtaacctg
    29641 acgccgcagg aagctatttt ccaggcctgt ctgctgcgtt ttcgcccgat tatgatgacc
    29701 accctggcgg cgctgtttgg tgcgctgccg ctggtattgt cgggcggcga cggctcggag
    29761 ctgcggcaac ccctggggat caccattgtc ggcggactgg taatgagcca gctccttacg
    29821 ctgtatacca cgccggtggt gtatctcttt ttcgaccgtc tgcggctgcg tttttcgcgt
    29881 aaacctaaac aaacggtaac cgagtaaatg acagatcttc ccgacagcac ccgttggcaa
    29941 ttgtggattg tggctttcgg cttctttatg cagtcgctgg acaccaccat cgtaaacacc
    30001 gcccttccct caatggcgca aagcctcggg gaaagtccgt tgcatatgca catggtcatt
    30061 gtctcttatg tgctgaccgt ggcggtgatg ctgcccgcca gcggctggct ggcggacaaa
    30121 gtcggcgtgc gcaatatttt ctttaccgcc atcgtgctgt ttactctcgg ttcactgttt
    30181 tgcgcgcttt ccggcacgct gaacgaactg ttgctggcac gcgcgttaca gggcgttggc
    30241 ggcgcgatga tggtgccggt cggcagattg acggtgatga aaatcgtacc gcgcgagcaa
    30301 tatatggctg cgatgacctt tgtcacgtta cccggtcagg tcggtccgct gctcggtccg
    30361 gcgctcggcg gtctgctggt ggagtacgcg tcgtggcact ggatcttttt gatcaacatt
    30421 ccggtgggga ttatcggtgc gatcgccaca ttgctgttaa tgccgaacta caccatgcag
    30481 acgtggcgct ttgatctctc cggattttta ttgctggcgg ttggcatggc ggtattaacc
    30541 cctggcgctg gacggcagta aaggtacagg tttatcgccg ctggcgattg ctggcctggt
    30601 cgcagttggc gtggtggcac tggtgcttta tctgctgcac gccagaaata acaaccgtgc
    30661 cctgttcagt ctgaaactgt tccgtactcg taccttttcg ctgggcctgg cggggagctt
    30721 tgccggacgt attggcagtg gcatgttgcc ctttatgaca ccggttttcc tgcaaattgg
    30781 cctcggtttc tcgccgtttc atgccggact gatgatgatc ccgatggtgc ttggcagcat
    30841 gggaatgaag cgaattgtgg tacaggtggt gaatcgcttt ggttatcgtc gggtactggt
    30901 agcgaccacg ctgggtctgt cgctggtcac cctgttgttt atgactaccg ccctgctggg
    30961 ctggtactac gttttgccgt tcgtcctgtt tttacaaggg atggtcaact cgacgcgttt
    31021 ctcctccatg aacaccctga cgctgaaaga tctcccggac aatctggcga gcagcggcaa
    31081 cagcctgctg tcgatgatta tgcaattgtc gatgagtatc ggcgtcacta tcgccgggct
    31141 gttgctggga ctttttggtt cacagcatgt cagcgtcgac agcggcacca cacaaaccgt
    31201 ctttatgtac acctggctta gcatggcgtt gatcatcgcc cttccggcgt tcatctttgc
    31261 cagagtgccg aacgatacgc atcaaaatgt agctatttcg cggcgaaaaa ggagcgcgca
    31321 atgaagttct ggcgacccgg tattaccggc aaactgtttc tggcgatttt cgccacctgc
    31381 attgtcttgc tgatcagtat gcactgggcg gtgcgtatca gttttgagcg tggctttatt
    31441 gattacatca agcatggtaa tgaacagcga ttacaactgt taagtgatgc gcttggcgag
    31501 cagtatgcgc agcatggcaa ctggcgcttc ctgcgcaaca atgatcgctt tgtctttcag
    31561 atcctgcgtt catttgaaca cgataattcg gaagataaac ccggcccggg tatgccaccg
    31621 cacggctggc gtacccagtt ctgggtggtt gatcaaaaca acaaagtgct ggttggtccg
    31681 cgagcgccga ttccacctga cggtacacgg cgacccattc tggtcaacgg tgcggaagtt
    31741 ggcgcggtga tcgcctcccc cgttgagcgg ttaacgcgca atactgatat caatttcgat
    31801 aaacaacagc ggcaaaccag ctggttgatt gtcgccctgg caacgttact cgcggcactt
    31861 gccacttttc tgctggcgcg cggtttactg gcaccggtaa aacgacttgt cgatggcacg
    31921 cacaaactgg cggcgggcga tttcactacc cgcgtaacgc ccaccagtga agatgaactg
    31981 ggcaaactgg cgcaagactt caaccagctt gccagcacac tggagaaaaa ccagcaaatg
    32041 cggcgcgatt ttatggccga tatttctcac gaactgcgta cgccattagc ggtgctgcgc
    32101 ggtgaactgg aagccattca ggatggcgtg cgtaaattca cgccggagac ggtggcgtct
    32161 ttacaggcgg aggtcggtac actgaccaaa ctggttgacg atctccatca gttgtcgatg
    32221 tctgatgaag gcgctctcgc ctatcaaaaa gcaccggtag atttgatccc actgctggaa
    32281 gtggcgggcg gcgcatttcg cgaacgattc gccagtcgtg gcctgaaact gcaattttcc
    32341 ctgccagaca gtattaccgt atttggcgat cgcgaccgtt taatgcagtt attcaataat
    32401 ttacttgaaa acagcctgcg ctatactgac agcggcggca gcctgaaaat ctctgccgag
    32461 cagcacgaca aaacggtgcg cctgaccttt gccgacagtg cgccaggtgt cagtgacgag
    32521 cagctacaaa aattatttga acgtttttat cgcaccgaag gttcccgcaa ccgcgccagc
    32581 ggtggttccg ggctggggct ggcgatttgc ctgaacattg ttgaagcaca taatggccgc
    32641 attattgctg cccattcgcc ttttggcggg gtaagcatta cagtagagtt accgctggaa
    32701 cgggatttac agagagaagt atgaccgagt taccaatcga cgaaaacaca ccacgtattt
    32761 tgatcgtgga agatgaaccg aagctggggc agttgctcat tgattatctg cgtgctgcga
    32821 gctatgcgcc gacgcttatc agccacggcg atcaggtact gccgtatgtg cgccagacac
    32881 caccggatct gatcctgttg gatctgatgc tccctggcac cgatggcttg acgctgtgcc
    32941 gggaaattcg tcgtttttct gacattccta tcgtgatggt gacggcaaaa atcgaagaga
    33001 tcgaccgcct gctcggactg gagattggcg cagatgacta catctgcaag ccgtacagcc
    33061 cacgggaagt ggtagcgcgc gtcaaaacca ttttgcgccg ttgcaaaccg cagcgcgaat
    33121 tgcagcaaca ggatgccgaa agcccgttga ttatcgacga aggtcgtttt caggcttcat
    33181 ggcgcggtaa aatgcttgac ctgacacctg cggaatttcg tctgttgaaa acgctctctc
    33241 atgaaccagg aaaagtgttc tcccgcgagc aattgctcaa tcatctttat gacgactacc
    33301 gcgtagtaac cgaccgcacc attgacagcc acattaaaaa cctgcgccgc aagctggaat
    33361 ctctcgacgc cgaacagtca tttatccgcg ccgtttacgg cgttggttac cgctgggaag
    33421 ccgacgcctg ccgcatcgtt tagttttagc gacatgactt tgttagccgg agatggcgtt
    33481 cagaccagat ccggcaacat tatcccacgc atggtcagca aactgacatt atctctccgc
    33541 cctggcaggc atcagaaaat cgctcatact ttaatcggta aacagcacct ttagatgctg
    33601 ttttcgatac gcaatttcaa tcaaggagtc attatggctg gttggtttga actcagtaag
    33661 agcagtgata atcagttccg gtttgtgcta aaagcgggca atggtgagac tatcctcacc
    33721 agcgagctgt atacatcaaa agcctctgcg gaaaagggca tcgcgtcagt gcgtagcaac
    33781 agcccgcaag aagaacgcta tgagaaaaaa acggcaagta acggcaaatt ctatttcaat
    33841 ctgaaagccg ccaatcatca aattatcggc tccagccaga tgtacgccac cgcgcaatct
    33901 cgtgaaaccg gaattgcctc cgttaaagcc aatggcacaa gccagacggt gaaagacaat
    33961 acgtaattac gatgccgggc gtgggttgtt ggcgtccggc tttattttct ctcatcacag
    34021 agttactatc cctcaagaaa ttattaacca tgaaatgagt tttctcctca taactgataa
    34081 ggaaaattat gctcaaagga ttgcgtacat tattttcacg cagggttttg tttaacgtcg
    34141 aaatgaataa ttctgttgac tgtgaattac ttctcatcca gcaacaactc caggcattga
    34201 tagccgaacc aggcaattat ttagcacatg attgtgatct caacgatact ctggttcttt
    34261 gccagcttac ccatcatcac aatggatata tacgagaaaa ggccgtgcag tatctggggc
    34321 acaaagagga tgttgcggct atagaagaat tattacttgc agctaatgac tgggtaaaag
    34381 aggttcgcct tgctgcaacc atagcattac gtcatttaat gaaggataag aatgcagcgg
    34441 catttgccgt taatctaccc atgatacgta accttctcca atgccagcgt tacgatcacc
    34501 gtgcatttgt agacgagata cttagatatt tattgacaga aaataaccgc ccacaattaa
    34561 cgtattggct tacttgccct gataaaaagc tctcccgcgc ggtattacaa acgctgattg
    34621 aatacggtta ctttaatgat gaatcttcac tgctttcaat actcgataaa cccgatgagg
    34681 gtttgcgaat gctggcgacc acccactggc tgcgtcaaca attaccatta agcgaagcgg
    34741 tattaactcg tctgttaaaa gaccgctggc cgcgtattcg acaagcaacg ttatttagcc
    34801 tcactgaccg agcaatagaa atgcctccgg cgttgcacag cacattactg ctggataaca
    34861 atatgttgat ccgcctacgc gctaaaaata tgcttcacga agtcatggat gttccgcaat
    34921 tctggcgtca tgttgtcact tctgcggaat atactccctc acagcgacgg gccgctttat
    34981 acgggctgga ttccattcat gatcccaata ttttaaaact ggcagaatgg ggattatcac
    35041 agaatgtatt cccactgcgt ttagccgcta tgcacattct tgcaaaagct aatcctcgtt
    35101 gtggcgtcaa agagactatt ctgaccacgc tggctaaccc ggacgccgcc gggttgcgat
    35161 ttatggttaa catctgtgta tggtgtcgcg ttccgctcac gttcgaggaa atacgtcagt
    35221 tgcaggaaaa cgcgccgtca gttaaacatg cttgcgcata ttgtcggctg tatcataact
    35281 tgaacaaatg ggacggttta attcttcttc ttcaatcgca acacaagcta actgaagaat
    35341 ttgcaggtaa acagctcgcg atatggcaac gcaatttcaa tctttcgggc attcaaccga
    35401 atgctcttca gcgtcagcaa cttcaggcgc tttttacccg caatccggaa cttcacaaca
    35461 gattatgggg ttacattcct tttaaataac ttcgaattat agacttacga tgacaaatgt
    35521 acctgctgac gagcggaacc aaaacgaaac aatacgtgaa gctttacgct tgctacgaga
    35581 aaaacttgcc gagggactac gcagtggcga agcccaaccg tgggaaaagg atctgttttt
    35641 acataaagtg aaggccggta acaaataaat cccccagtct tttccctcac atttgcactg
    35701 gagccaaccc cgccgattgc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
    35761 cgcttcatca ggtgaagcgg atctgacctg tcatcagaac gagagaatta tgtttaaacc
    35821 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
    35881 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
    35941 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
    36001 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
    36061 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
    36121 gcgtgagtac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
    36181 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgaact
    36241 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga tcgagatctt
    36301 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
    36361 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
    36421 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga tcccggtgca
    36481 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
    36541 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
    36601 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
    36661 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
    36721 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
    36781 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
    36841 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
    36901 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
    36961 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
    37021 catggaaaac gccaaaggtg tagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
    37081 gctcccggtc ccgcaggatc ttgaactaaa ttacgcgctg ctgatgcgta atttctccgg
    37141 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
    37201 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
    37261 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
    37321 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
    37381 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
    37441 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
    37501 tcactctcca catttgaatg tcagacgagc gacaccatgt aatcctacac cttctgtctt
    37561 cagctcaact atttgcattt ttttgccctg agtaacacag aaatgagttg catcattttt
    37621 tactatattt tctgcaccag atattctact cctggctaaa gaagcttcgg cttcggtgta
    37681 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
    37741 acttgtctca tcattatgca cagcacaacc agacataata aaaatactaa ttaatgatat
    37801 agcagctatc tttttcatct cacctttccc cattaaatac caacgacact ctctagtgtt
    37861 taaatataat aatggctgat cattatattt gaataggatt ataataaatg ttctgtacaa
    37921 catttcctac ataagtagga cttacagaca tgaaggccct ttccaggtaa ctccatgggg
    37981 ctttaatata tttggaatag tatatttaag atgccactgt ttggttgaat attaggcata
    38041 tgctcttttt tgaaatttat cggtggctac cgttaatatt cgctgttccc attgcaagct
    38101 cctggtggta accactgaat cctccatact tgaactgact ttttatcctc cgactttcat
    38161 cctgttctga ctccaccttt tcttttctgc tctacactat ctacagacca atcataaagg
    38221 cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggc aaaagtactg
    38281 acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg acgatccatg
    38341 tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc cggaagttgg
    38401 gcgtcgcaac ggtaattgcc ggtggtggcg atggcaccat taatgaagtt tctacggcgt
    38461 tgattcagtg tgagggggat ggcatacccg cgctgggaat tttgccatta ggaaccgcca
    38521 atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg aaactggcaa
    38581 ttgccggtaa cgccattgcg atagatatgg cgcaggtcaa caaacaaacc tgttttatta
    38641 atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa aaattaaaag
    38701 ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat actctgcaac
    38761 cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc ctggtattgg
    38821 tattggtaac gggcgtcagg ccggtggcgg tcagcaattg tgtccgaacg cgttaattaa
    38881 cgatggcttg ctgcaactgc gcatttttac cggcgatgaa attcttccgg ctctcgtatc
    38941 aaccttaaaa tctgacgaag ataacccgag tattatcgaa ggcgcttcgt cgtggtttga
    39001 tatacaagcc ccacacgaaa tcacttttaa tcttgatggc gaaccgttga gcggacaaaa
    39061 ctttcatatt gaaatacttc cggcggcgtt gcgttgtcga ttaccaccag attgtccatt
    39121 attgcgttaa tcaaactatt ctctcttgaa ccagggagaa tccgaaatgc cggatgcggt
    39181 gtaaatacct tatccggcat acagaacaat actacaccgt aattaatttt acccgtgtct
    39241 tctgcaatgc cagtttatcc acctctgata cagcatcatc ggtaataata atgtcaattt
    39301 tttcgattgg cagtacctga ttaaaaccgc gacggttaaa tttcgatgaa tcgagaacgg
    39361 caatcacttt attagcggct gtgaccatcg caccgctaac ggaataaccc tcattaaagg
    39421 tcgtaatacc attaaccgca tcaataccat cagcaccgac aaacattaaa tcggcattaa
    39481 tatcctgcag ggaacgttca gcaatagatc catgcataga tcgcgtttta tggcggaccg
    39541 tgccaccgca gacgaccaga gtaatgtctt tattttcgga aagggcaaac gctgccggga
    39601 gactgttggt aatcaccgtg atatttttag cggtcattaa tccttcggcg ataagcattg
    39661 tggtacttcc gctgtcgaga ataacggtca tcccttcatg gatcattttg actgccgcct
    39721 gcgctattct gttttttggc gcgctggcga gctgaaatcg ctctttaaac ccgacttcct
    39781 gggtctcggt ttcactatta ccggacatta ttttcgccgc accgccatga aagcgcgtaa
    39841 ccacgccttt ttgttcgaga aagcgcaaat cggcacggat tgtcgcttcc gaggcagcaa
    39901 atactcccgc cagatcctga acaagcacgg ttccctgttc attcactaat tggatgatct
    39961 tattccttcg ctcgaatgaa ttcatcgtca tttacccata ttcaattgtg gctagtgtaa
    40021 acgaaggggg accattggtg aatgatctgt tttgcacaat accgggccag catcacgctg
    40081 gcccgcggtt tcatggaatg agcaacactt tgcccggcat ggcattacga gcgatgtcac
    40141 gcaccgcctg ggtgaagctt tcaaagcttc cacggtgagc gattaatggc tccaggctta
    40201 acttacgttc tgtcagcaac cggctcgccg tttcccactc ctgccccggc caagggctgg
    40261 aatagttcat ccaactgccg ataaccgtca gctctttacg caatattttg ccaaacgttg
    40321 ccgatgttaa atgcagatcc tgatgcaacg tgcccaccag cgccagttgg gcatgaggac
    40381 cggcaatctc taccgccagt tcgacagttt gcggtacgcc agccgtctcg aggataagct
    40441 gattaaaacg cagttcgcgt aaaacgctct gcatttgcgg cgcgctcatt tcgctactgt
    40501 taaatgtttg catcgaaccg aaagattttg ccagtgccag tttttctgag ctaatgtcga
    40561 tagccgtcac actctttgct cccagcgcga cagcgcactg aatagccagc aggccaatgg
    40621 ttccggcacc aataataata acgtttttat tctcacaacc ttgcgctaaa tgaaaagcat
    40681 gcagaccaat ggtaatcggc tcaataaaag ccccatcctc aataggcata tccgtgggta
    40741 gagcaaagac attttttcgc ttaacgacaa tatattcagc aaatccacca tcacgccgcg
    40801 agccaataaa atcatatttt gcgcactggg aataaaaccc tttcagacac tctggacaag
    40861 taaaacaggg taataacggc acacaggcaa ccgcatcgcc agggtgtaaa tcatcaacac
    40921 cggatcccac cgcatcaata tagccgctaa attcatggcc taacgttatt ggataataat
    40981 gtgcaccatt tttaaatatc ctgggtaaat cggaaccaca taagcccgag ctggcaattt
    41041 ttacccgcac ctcatcctga tgtttaattt caggaatgac gctttctgca acgcgcacga
    41101 taccatcagt atcattcacc actgatttca taaaaactcc tgattgttaa gggggataaa
    41161 ctccccctca gttaaaaatt attctgcgag aacggctttc tcttgtttaa taaaaccacg
    41221 cgctctacgc caggtaatga aaataccggt cagataaatt gcgccgataa taatgaaacc
    41281 gggaatattt tgcggggaga aaacctgaat cagtaaccag gtaatgggag aaccgccctg
    41341 atccattgaa gccaccatac ccccggcttt taacgcccca gcattagccg ccagttgggt
    41401 gtgcaaacca atagtttgcg tcgcgatcca cagagtgatg ctcatgatga tgacacccga
    41461 gattaaggtg cggaacagat ttccacgatg cacggcgact gccatcgcca caaagaagcc
    41521 gatggtggca agatcgccaa acggcagtac ctgattaccc ggaacacaaa cagcaattaa
    41581 aatggtgagc gggataaaaa tcaggcttac cgataccacc gccgtatgcc ccagcagtaa
    41641 tgcgggatcc aagccaatca ggaactcctg accgccgaac ttcgcctgta aacgactacg
    41701 ggcctgctta gcgatgggcg ttaaaccatc catgatgggt ttaatcaccc gtggcattag
    41761 cagcatcact gccgccgttt ttaccgccag ctgcaataca cctttgacat cgtaacccgc
    41821 gaggatgccg ataatcagcc ccatcacaaa acccacggtg acaggttcgc caaaagggcc
    41881 aaatttgcgc tgaatatcat cggcgctaaa tttaattcgg ttaacgcctg ggattttctc
    41941 gatgatagca tcgaccagca ccgcaatcgg ccccatatac gccgacgtac cgtgcggaat
    42001 agcaatacct tccagctcaa agaaatttcg ggtatcgcgg gcaaaccagt cgccgagctt
    42061 ataaacaaac gccgcgtgaa ttaccacacc cgccatccct atcatccatg aaccggttgc
    42121 cagatgcagc aacgcgccgg tgaaagtcat atgccagata ttccagatat caatatttac
    42181 cacccgcgtc atacgggtca gtaacatcgc cacgttaacc agaatagcaa tcggaatcgc
    42241 caccagcgca atttgcgacg cccaggtcat tggtgaagag cccggccagc cgacatcgac
    42301 cacatgcaga ttcaggtcga aattttccgc catcgctttc gccgccggac caatggaatc
    42361 cagcattaag ccaatcacaa ggccaatgcc aacaaagcca atccctatat gaagacccgc
    42421 tttaaagcaa tcgcctgcct tcatgcctaa tattttagaa aaaataatga tgacaatcgg
    42481 cagcatcacc gtagggccga ggtcgagaat ataacgcatg acttctgaaa acataggtca
    42541 cccctgtaag atagtcagaa ttttattttg taatgcttcg ataccgacac cagaaacaaa
    42601 aggcatgccg tgaactaacg gaatatcgcc aaaactacga tccaccctgg cagtagtgca
    42661 tatcaaatgc acgccatcca tataggtttc tatttcatta acccgacact ggattaattc
    42721 aacaggaata ttatgattct gacacaattc tttaatttct tccgccgcca tcgtagaggt
    42781 cgcaaccgcg cctccgcaag cgacaataat cttgcgtttc ataaatagtc ctttttattg
    42841 ttacagaggg atttattcgt ctgaatctaa aacatacttt gtgaagtatt cctttaactg
    42901 ggtttcagga agagtgatta gtgtctcgac gatatcgggc tgttgtaact tgccaaataa
    42961 acagcgtaaa agtttcaatt gctgctgcgg attttccaca atcaacgcaa taaccaacga
    43021 tacctccacg tcgttatcat catccgcttg ctgaaaatga actttatttg ttggccttaa
    43081 cagataaatg gctgacgact tagcatgaat cgcctcacaa tgcggtattg caatggcgtg
    43141 ctgctcaagc attatcccgg tagggaattc tgcttctctg gcaattaatg cctgtggcca
    43201 ggtatcatga accacacctt tggcgagcat ctcattaccg atatgggtta aaacttcgct
    43261 acgatcgaca aaagaaattc cgctacgaac aaacaggtta gtcatatacc gtccttattc
    43321 cgcacagccg tagcgatagg cgcgcaaaac atcataaatt ttatccataa tcagctgatg
    43381 cggtattgct gataattccc cggactgaat gcgttcaaat tgtttgggaa gatactgact
    43441 aatcatgccc agtgggatgt ccacgccttc aagattcacc atcatcgttt cgacgctatt
    43501 tttaatccga ctatgcggcc agtaataacg aatacgatcc gacaggctgt aacgaatatc
    43561 cagtaatgaa tcgttaaaac ccgtacgata atattttttc cagtattgcg gttcgtcgag
    43621 catcacttct tcaattaccg ccaggcaacc gctgcgattt tcaggggcga taagttcctg
    43681 ctcaatttgt gccagtgcaa atatcgcctc gcgtaaagca aaggttaatg cgggaccgac
    43741 tttcaatatt gcaaagtgat cgcggactaa ttcccaataa gccgtccggg tctggtaatc
    43801 ggtagaatgt gcttcataaa ccattcgggt gttttctatc cattgcgcca gcggctgcgc
    43861 ttcctgcggc tgataatgga taatattgct gtgatcaaat tccacgcccg gctgcaccac
    43921 gatggcaatc acgcgtgtta acgcctctgt cagcccacgg acaataaagg ccttttgatg
    43981 cgtacgtaaa gtattggcgg catcttcaac atgggtgatg tgtactgact gaatggcgct
    44041 ggcctcaccg cccggaaccg gaacttcggt gccaatgaca tagctcagtt gctcacgctg
    44101 gcaatctgtc gccacacttt ccgcagcaaa gcaaagcaca gcagctcgtt ccgcaaccgt
    44161 ttctggtgct aacggtatgg gatcccccgc gcaggacatt gacgcatcaa gatgaatttt
    44221 actgaagccg gcacgaacat attcctttac cagctcgacg gatttttcca tcgccgcatc
    44281 cgcattttct tgctgccagc agtttggccc cagatgatcg ccgccgagaa taatgcgttc
    44341 gcgtgcaaac ccaactttat cggcaatcgt aaaaacaaat tcgcgaaagt ctgccggtgt
    44401 cattccggta taaccgccaa attgattgac ctggtttgac gttgcttcaa tcagcacttt
    44461 gcgcgtgctg ttgcgatcaa atgccagcgc cgcttcgata accaacggat gggcagaaca
    44521 gactgaacat atgccgatat gttcaccagc tttatgccgg gcaattaacg ttttcatgtt
    44581 ttttccttat taaatggcag gtgcgttatg ccctgccctc gcagccacaa tcggcaatca
    44641 ctttgctcac cacatcgcgc attgcggatt tagccgactg caaataatcc cggggctcgg
    44701 tcgcttcagg gtgctcggtc aggtaatttt ttaacgcctg cgagaaggca tttttcagct
    44761 ccgttgcaac gttgattttg catatcccca gtttgatggt ttgctgaata tccttagtcg
    44821 ataaccctga cgcgccatgc agcaccagcg gtaagttcac ccactggcga atgttctcca
    44881 gtctagaaaa atcaagcgcc ggtgcgctgg catacatccc atgagccgtg ccgatggcga
    44941 ccgccaggga atcaattccg gttgcctcgg caaattcacg cgcctgagcg gggttggtat
    45001 aaaaagcatc ggcttcattg acttgcacat catcttcctg gccgccaagt tgccccagct
    45061 ccgcttcgac gctgacatca aagcgatggc aaaaatccac cacctctttg acccgtgaaa
    45121 tattttgcgc aaaaggcaaa tgcgaggcgt caatcatgac tgagcgcacg ccagaacgaa
    45181 ccttctgagc gatatcgtca aatttcgtgt gatggtcgag atgaattgcc agtggatggt
    45241 gatattgctt cgccatcgcg ctgaccagcg ccaacagatt ttctgtacca gcatgagtaa
    45301 atgtgccagg cgttccggcg atgatgaccg gcgcatgcag gttggcagcg gtttccacca
    45361 ccacttgcat cgtttcgaga ttgtgaatat tgaatgccgg aaccgcataa ccgccgcgct
    45421 gtgcgttgtt cagcatctgc tttgtcgata ccacgtgcat tttcatatcc tgtcgtttgt
    45481 tttcgttttc aaaatataat gaaattattt gtttttaaat atcgagataa cgatcacaaa
    45541 aacgacaata tgaaaattat tcgaggagtg aaaggcaaaa aaacggcctc ccgataggga
    45601 agccgtagca aagtgcgcgt gtttttatgc cggatgcggt gtaaacatac gtggcagtgc
    45661 tatgtaggca tgataagacg cttccgcgtc gcatcaggca taggttgccg gatgcagtgt
    45721 aaacgcctta tccgtcctac ggggtggtgt tgttcgtata aggcgtatca ggcaatagta
    45781 attttgctat cgagataaac gtcctgcacg gcgttaatca gtttcacgcc gtcagccatc
    45841 gatttcttga acgccttacg tccaagaatc agccccattc cgccagcgcg tttgttgata
    45901 accgcagtac gcactgcatc actgaggtca gtttcaccgc ctgcagcacc gccggagttt
    45961 atcaacccag cccggcccat atagcagtta gctaactgat aacgcaccag atcaatcggg
    46021 ttttcgctgg ttaacttgct gtacacgcga tcgtcggtat aaccgtaatt aatcgcttta
    46081 tagccgccgt tattttccgc cattttttgt ttgacgatat ctgcgccgat ggttgccgcc
    46141 agatggtttg cctgaccggt caggtcggcg gaaacatggt aatcaacgcc atctttctta
    46201 aaggcggagt tacgcaaata ggcccacagc acggtcacca tgcccagctc atgcgcacgt
    46261 tcaaaagccg cagaaatttc ttcaatctgg cgacgtgact cttccgagcc aaaatagata
    46321 gtcgcaccaa ccgccaccgc gcccatgttg aaagcctgct ccacgctggc atacagcgtt
    46381 tgatcgtagg tattcgggta acttagcgtc tcgttgtgat taagtttgac gagaaatgga
    46441 atgcgatgcg catagcgccg cgataccgac gccaacacgc cgtaagttga taccacacag
    46501 ttacagcccg cttcgatcgc cagttcaaca atgtttttcg ggtcaaagta gagcgggtta
    46561 gcagcaaatg aagctccggc agagtgctca acgccctggt caaccggcag aatagaaaga
    46621 tatcctgtgc cagccagacg cccggtgttg tacaacgtct gcatattacg taacaccgct
    46681 ggcgggcgat tattgtcaat cattacgcgg tctacgtagt catgtccggg gagataaagc
    46741 tggtcagaag gaattgtcat acaacggtgc tgtaaaaggt tgtcggcgtc tttgccaagc
    46801 aactgcgcaa tatctgtcat tactatgctc ccgtaaattc cgattggata tcggctatgg
    46861 attgtcctgg cccgcctttt gcgggcaatc ataatcctgg tcgttacggg cgagtttttc
    46921 catcttttgg atgttttttc agcgttttct gttggctcga ttcatcagaa aaaatgttag
    46981 cgcggtcaag ttttcaccgt aaaggcattt aaaaggtatt attaagtggt attgtcatcg
    47041 cgtaccttac attacctgtc atgaaggaat taaaagatga aaacaacagc aaagctgtcg
    47101 ttcatgatgt ttgttgaatg gtttatctgg ggcgcgtggt ttgtgccatt gtggttgtgg
    47161 ttaagtaaaa gcggttttag tgccggagaa attggctggt cgtatgcctg taccgccatt
    47221 gcggcgatcc tgtcgccaat tctggttggc tccatcactg accgcttttt ctcggcgcaa
    47281 aaagtgctgg cggtattgat gttcgcaggc gcgctgctga tgtatttcgc tgcgcaacag
    47341 accacttttg ccgggttctt tccgttactg ctggcttact ctctaaccta tatgccgacc
    47401 attgcgctga ctaacagcat cgcttttgcc aacgtgccgg atgtggagcg tgatttcccg
    47461 cgcattcgtg tgatgggcac tatcggctgg attgcctccg gtctggcatg tggtttcttg
    47521 ccgcaaatac tggggtatgc cgatatctca ccgactaaca tcccgctgct gattaccgcc
    47581 ggaagttctg ctctgctcgg tgtgtttgcg tttttcctgc ccggcacgcc accaaaaagc
    47641 accggcaaaa tggatattaa agtcatgctc ggcctggatg cgctgatcct gctgcgcgat
    47701 aaaaacttcc tcgtcttttt cttctgttca ttcctgtttg cgatgccact ggcgttctat
    47761 tacatctttg ccaacggtta tctgaccgaa gtcggcatga aaaatgccac cggctggatg
    47821 acgctcggcc agttctctga aatattcttt atgctggcat taccgttttt cactaaacgc
    47881 tttggtatca aaaaggtatt attgcttggt ctggtcaccg ctgcgatccg ctatggcttc
    47941 tttatttacg gtagtgcgga tgaatatttc acctacgcgt tactgttcct cggtattttg
    48001 cttcacggcg taagttacga tttttactac gttaccgctt acatctatgt cgataaaaaa
    48061 gcccccgtgc atatgcgtac cgccgcgcag gggctgatca cgctctgctg ccagggcttc
    48121 ggcagtttgc tcggctatcg tcttggcggt gtgatgatgg aaaagatgtt cgcttatcag
    48181 gaaccggtaa acggactgac tttcaactgg tccgggatgt ggacttttgg cgcggtgatg
    48241 attgccatta tcgccgtgct gttcatgatt tttttctgcg aatccgacaa cgaaattacg
    48301 gctatcaagg tcgatgatcg cgatattgcg ttgacacaag gggaagttaa atgaaaacag
    48361 aacgtattct cggtgctctt tatgggcagg cgttagggga tgcgatgggg atgccctccg
    48421 agctttggcc acgcagccgc gttaaagcac actttggctg gattgaccgt tttctgcctg
    48481 gaccaaagga gaataacgcg gcctgttatt ttaaccgcgc cgaattcacc gacgatacct
    48541 cgatggcgct gtgtctggcg gatgcgttgc tggaacgtaa aggcaagatc gatccggatc
    48601 tgattgggcg taatatcctc gactgggcgc tgcgtttcga cgccttcaac aaaaacgtgc
    48661 taggtccgac ctcgaagatt gcgcttaacg ccattcgcga cggtaaaccc gttgctgaac
    48721 tggaaaacaa cggcgtaacc aacggcgcag cgatgcgcgt ctcgccatta ggttgtttgc
    48781 ttccggcgcg cgatgttgat tcctttattg atgatgtggc gctggcgtcg agcccgaccc
    48841 ataaatccga tctggcagtt gcaggtgcgg tagtcatcgc atgggcgatt tctcgtgcta
    48901 ttgacggaga aagctggtca gcgattgttg attcactgcc ttcaattacg cgacatgccc
    48961 agcaaaaacg caccaccacg ttcagcgcct cactggcggc gcggctggag attgcgctga
    49021 aaattgtgcg caatgccgac ggcaccgaat ccgccagcga acagctttac caggtcgttg
    49081 gcgcaggtac cagcactatt gagtccgttc cgtgcgccat tgcgctggtt gaactggcac
    49141 aaaccgaccc gaaccgttgc gccgtcctct gcgctaacct tggcggcgat acagacacca
    49201 tcggtgctat ggcgacggca atctgcggcg cgttgcatgg cattaacgct atcgatcctg
    49261 cgttaaaggc ggagctggat gcggtaaatc agcttgattt caaccgctat gccacagcgc
    49321 tggcgaaata tcgtcaacaa cgggaggcgg tatgagcggc gctcgattac acacgctgct
    49381 gcctgaatta accacgcgtc agtcggtgat ggtcgtcggc gcggcggtca ttgacgtgat
    49441 cgccgacgct tatgccctcc cctggcgtgg gtgcgatatc gaactgaaac agcagagcgt
    49501 taacgttggc ggctgcgcac tgaatattgc cgtggcgtta aagcgcctcg gcatcgaagc
    49561 gggtaatgcc ttgccgctcg gtcagggggt atgggcggag attattcgca accggatggc
    49621 gaaagagggg ttaatcagcc tgatcgataa cgccgaaggc gataacggct ggtgtctggc
    49681 gctggttgag ccggatggcg aacgtacttt tatgtcattc agcggtgtgg aaaatcagtg
    49741 gaatcgccag tggctggcgc gattaaccgt tgcgcctggc agcctgctct atttttccgg
    49801 ttatcaactg gcctcgccct gcggcgaatt gttagtggaa tggctggaag agctgcaaga
    49861 cgtgacgccg tttatcgatt ttggcccacg tattggcgat atcccggacg cattactggc
    49921 gcggatcatg gcctgtcgac ctttagtgtc actcaatcgt caagaggctg agattgccgc
    49981 cgaacgtttt gctttatccg cagagataac aacacttggc aagcaatggc aggagaaatt
    50041 tgccgcgccg ttgatcgttc gtctcgataa agaaggcgca tggtatttca gcaacgacgc
    50101 ttctggctgc attccggcat ttccaacgca agttgtcgac accattgggg cgggcgacag
    50161 tcatgccggt ggcgtacttg ccgggctggc ctctggtctg ccactggcgg atgccgtatt
    50221 actgggcaat gcagtggcgt catgggttgt cgggcatcgg ggcggtgatt gtgcgccaac
    50281 gcgcgaggaa ctactcctcg cacacaaaaa cgtatagatc gctgcgacag tggctaatgc
    50341 tgtactcaat aggccgttgc tgttggtcaa gcgccacttg cttgatcacc agcaccggta
    50401 ttttgctatc taactgaata tgtgactgaa actcggcatc cggcatccgg gcgctaacgc
    50461 gggaacgcgt acgttgtggg taaatgtgct ggctgcggaa atagtcataa agtgaaatcc
    50521 cgatggcatc aacatcatga attaaatgcg ccggaaccca cgattcctca atcgataccg
    50581 cttcttcatc gacatagcga atacgcttaa gcaaaaagac atcacttcct gcttcgaccg
    50641 ccagctgctg cgcgacttct tcgggacatt tcacgacacg cttgttaacc catagcgtat
    50701 cgggcttttt cccgcgcaat accacctgct gagaaaaacc tcgcgcttct ttcaacgagt
    50761 attcgaagat gttgttgatc tgcgtgccgt aaccgcgcga acgcgtcacc acaccctctt
    50821 cttccagcgc ctgcatcgcc ttgcgcaccg taatgcgcga aacgccggtt aactgactta
    50881 aatcacgctc accgggcaaa atattgccat gctccagcac cccgctgcgc acggcatttt
    50941 ttaccgtttc ggcaaactta atataaagcg gcgtgttatc cgccgctaaa atacgctcgt
    51001 tcagttgggc aatcagctgg gtatgcgctt gttccatttg tcttgttcct gccggggagt
    51061 ctggtgggag tatatcactg cttcttattt aaggcgtttc tttaatccca tcaacgaatg
    51121 cctgcaactc ctccaccgtg ccattaaata cattaaaatc caccggacca ttgatgccat
    51181 ctacctgtcc acggtcggaa tgctgccaga agcgccaggc catgccgtca ttgtccggac
    51241 gacgttgata atagtgagcc acccaccacg gatattcatt gaaatagccc gccagattgg
    51301 tgtgataaaa aacggctcct gagtaaataa tcggcttttt tcccgtactt ttttcgacca
    51361 tttttagcca ctgacttact cgtttgcgta attcttttgc cgataatttt ccccgttctt
    51421 ctacgtccag taccgcaggg aaatcacctt gtgagaaatc caccgtttgc agaaataatc
    51481 gtgcctgaac cggcgctgct accgacgggg agaaataatg atacgccccg cgcagcaggc
    51541 cattttcgcg gcttagttgc cagttacgcg aaaaatacgg gtccaccagc ttttcgcctt
    51601 ccgtcgcctt aataaaagca aactgtaagc ggataccgtt gtcgcgcatt tttgccaccc
    51661 gctgccagtc gatccgctcc tgccagcgag aaacatctat tccgtgaatg gtgtaactgg
    51721 caggaatgcg aatagcgaaa gatttaaccg ggcgataacc gtagaaatgg atgtagtctg
    51781 taatctgaac tgctgtcgaa tagaaaaaat tcaccttctg acgcggataa attgcaacga
    51841 tggaaattag cccaagagcg cataatagag aggtgagttt ttttcgactg gtaattctta
    51901 gctgcattgg tttcatccct gaatgtcagt gccaaaggct gacaataacc aaagcagcta
    51961 tagtacggtg ctcaccacca ggcgtggaag tggtgaaccg gaccaatacc gtgaccaact
    52021 tccagcgtgt cggcctgggc taatgctgat gaaagccagc tttttgcctc ctgtacggtg
    52081 tcagcccagt ttgtatggcg cgggcgtagt gcagccaacg ccgcagagag cgtacaacca
    52141 gtgccgtggg tgtttttggt cataatgcgc ggtgcggtaa accgttgttc accctcgcgg
    52201 gtaaacagcc agtccgggct ttgctcatca tccagatgac cacctttcat tagcactgcg
    52261 ccacagccca tcgccaacag cgatcgccct tgttccagca tttcctgttc agtgcgcgcg
    52321 tgtggcgcgt cgagcaaggc ggcagcttcg ggcaagtttg gcgttattaa tgaaacctgt
    52381 ggcaataatc gactgcgcag cgtagcaacc gccgaaggtg aaagcagcgg gtcgccgctt
    52441 tttgccagca taacggtgtc gagtaccacg tattggatct gataacgttg caaccgttct
    52501 gccaccgctt caacaatatc ggtttccgcc aacataccga ttttagtggt gtcgattcgc
    52561 acatcgctga acaccgaatc gagctgggcg gcgacaaaat cagactcaat gcgatacacc
    52621 gactgtacgc cacgggtatt ttgcgccacc agtgcagtaa taactgagca accataagcg
    52681 ccaagtgccg agaaggtttt aagatcggcc tgaatccccg caccaccact cggatcagta
    52741 ccggcaatcg tcagagcgtt aattcgtttc atgcctgcac ctcctgcgtc aattgccaga
    52801 gcgcatcaag gaaatgtgga acaaaactgc ctggcccctc gcttctggcg actgcgcgtt
    52861 ctccggcttg tttcatccag tgacaggcag atgcgacatt ttccagcgta tcgcctggta
    52921 acgcacagca ggcagcgaca accgccgata atgcacagcc agttcctacc actttggtca
    52981 ttaacggatc gccaccgtga ataccaatga tacgatgtcc atcggtaaca taatccatct
    53041 cgccagtgac cacgacgatt gcgccagttt cccgtgccag tgtttgtgca gcgggtatcg
    53101 cgttagctgc ggcgtcagtg gtatccactc cccgtccacc attagcaatg ccagctaatg
    53161 ccatgatttc cgaagcatta ccacgtatag cggttggctt atgaggcaga agttccagac
    53221 aaaaacggcg gcgataatcg agtgcaccaa ccgctactgg atcaagcgtc cagggtgttt
    53281 gagagctttt tgcttgctca acggcggcac acatcgactg tgcgcgtagc tgcgtcagcg
    53341 tgccaacgtt aatcagcaac gcactagcaa tagccgcaaa ctgactggcc tcttcggttt
    53401 cgataaccat cgctggcgat gcaccgagcg ccagcaaggt attggcagta aaggtttgca
    53461 ccacatcgtt ggtcatgcag tgcacaagag gggaatgttg gtgaaaaagg tgtaacgcgt
    53521 gcgcagattg cgctgaactc tgcaggtcga cttgcatagt ttgctcctgc cataacgtga
    53581 agaagcaatg acctggtggt ccgtgacttc cctacgctgg cattatccag atcaggtaat
    53641 acgggtattt ctcagccttc acaaagaagg gcaccccgag tcatttaaaa caaaatcaaa
    53701 agattgcgat taacgtcccg ttaatccctg ttctggatca tgccagcgga aagccaaaga
    53761 ctcaacccta aaaaatcctc tgtgtcccag tttgtgtctc acgttgtggc ccacagataa
    53821 atggcaggca caaaaaaacc gcccataagg cggcttaagt aaattattca gtcgtgaaga
    53881 tttaaatgaa atctattttc tgcatacgct ttcttcatgc agtcgaagtg cttttgccaa
    53941 ttccaattgg aatgcaaagg aaagacggga acggctgatc ttgataccgt caataatgat
    54001 gtatgtaggg taaacagtga ccatgcggcc caagtattgg aagtgtttag ggtggtttac
    54061 tttatcgtta ttcattgtgt gccatccgtc tgggtggggg taatcccggc aacggatgat
    54121 tgatctaaaa tcgatcaaaa tcaataactt aacagatcac caaatccagg ggggtgtaca
    54181 ttggcgagcc tccctcagcc taacctatac acgaccataa aataggggcg gattccttcc
    54241 agttgggtgg gaaaatatct gggagtttgg atacagtagc caggcagggt aaccagaccc
    54301 atagatgaga atgtttatta ttcccaaata tccaggcgag gggcctgctg tggctctctt
    54361 tacgcgccgc tgtggcgtct tctctcctct ttctatacaa tcccatagct aacccattgc
    54421 gaggcttaca gagcgttaca gagcgtccgt gtgaatattg cataaatgaa agtggtgaaa
    54481 ggtccgattt ggtcaacttt tagacaacta tttaacatat gcgcaacagg ttagacctgt
    54541 accctttcaa taggtccgcc tcctgggcgc tgtgtacgcc ccataactgt ggctcttctg
    54601 tacccatccg ggagccattc cacagagtaa actgtaaaac tgatctgcct gtgagtaacg
    54661 cccttttcct gtggataact tccacgcact aaccccataa actccccttt tgaggcgtcc
    54721 gcctcttcag gccctttctt gtggcaaaaa cacaaaaagg gtttagggcg gtggctaccg
    54781 cccctgtaga tgggaatgag tcgcactcga aagagggatt gagaaccaat tctcaagtga
    54841 taatcgatct catttgtgtt cgaagttttc cccagaaaaa taattttggg gttaagcatg
    54901 taaaatgcta cacacttgca ctttgtaatg ataattaatt gaccataaaa acaaatagtt
    54961 agaagcgctg ggggagtccc actagttaca caattgattg ctttttgagc taaaactgat
    55021 tcttttaccg ggtatacaat ccatttgatc gccctttacc acatatttaa acggatttaa
    55081 agccatttat ttagggggtt agcaaagtgt tgattccatg gttacagata acaaaaaagc
    55141 ccgccggggg ttagccgggg gcctgtggtt gggggtgtca aacgtggtta gttcggcccg
    55201 ccctgcatta gcatgttgaa agcctgctga ttattgctct caacttcaga gcgtaccaga
    55261 tccttaacct atccatcctg cactttcaca ttaacgttca cgtttagcgg ctggtttagc
    55321 cacgctggcg gcgtcatcag tggattacct gccatatcaa ctttaagctg tggcgctgta
    55381 atcgtcggcg taggaggtac ataagttgga ttggagtaag agcttaccca ccctgtttta
    55441 tctgctggtt tggcaaagtc tggattagtc ggaaggtctg gactatggag gaaatcccac
    55501 agtttaccca gcgggctatc ttttttcaga ttgcccccgg cggtggtgtc ctgtccatac
    55561 atggcgtcgt tagcgtcatg accttatccc aggcataatc cccacctacc gaaccgaccg
    55621 caatgctcca gccaacagga ccgccagaga gagccgccgc gccgcctacc ttgccaactt
    55681 tcggaccccg acccggtttg tttggcttgt ctttaccttc cccaccagat ccgccagtag
    55741 tagcggcttc aatgccacca atagcggcga tccctttcat gacccccagt aatttggtta
    55801 gtgggttaag catggtcaac aaccatttaa aggctacgcc caacttccag atagacccgg
    55861 cgaacatagc caccccggcg gcgtatgctg ccatttcgcc ccaggcttta agctgctcac
    55921 cagtgatccc catcttgctg gcgtaatact caataatgcg gaaggtcagg atcacagtgt
    55981 catgcaactc tgtaaacgca tctgtggcac cctcaacgat tttcccggca atatgcccga
    56041 acatctcaaa ggccccacca gagccgttaa cagcggcgct gatagcgtcg aaagcgctgg
    56101 ttaattcgtt gccgaagcca gaagcaaaga agatgttcat cgcgttctgc atactggttt
    56161 gcaggcgctg ccagcttgcc cggttggact tcatagcctt atccagagcg ccgccgttac
    56221 gagctgcggc tttcatctgt ttagccactt ccgggaggat gtccttagag aacagcttcc
    56281 cttgttgcat caggtcaaac agatcctttt ctgtaaggtt ggttgtccct ttcatctggt
    56341 tgagagcctt aacgaacaga cccacagccc ccgggagagc ttcggacaac tggcctttga
    56401 gttcttcact c
//
LOCUS       DAJTNN010000006        54608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTNN010000006 DAJTNN010000000
VERSION     DAJTNN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.006"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            18..908
                     /gene="cyoE"
                     /locus_tag="OO150_000347"
     CDS             18..908
                     /gene="cyoE"
                     /locus_tag="OO150_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5514613.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            1057..2421
                     /locus_tag="OO150_000348"
     CDS             1057..2421
                     /locus_tag="OO150_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5514614.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(2549..3040)
                     /gene="yajQ"
                     /locus_tag="OO150_000349"
     CDS             complement(2549..3040)
                     /gene="yajQ"
                     /locus_tag="OO150_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5514615.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            3208..4119
                     /gene="panE"
                     /locus_tag="OO150_000350"
     CDS             3208..4119
                     /gene="panE"
                     /locus_tag="OO150_000350"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5514616.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSANLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            4082..4672
                     /gene="yajL"
                     /locus_tag="OO150_000351"
     CDS             4082..4672
                     /gene="yajL"
                     /locus_tag="OO150_000351"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5514617.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLQVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4726..6174)
                     /gene="thiI"
                     /locus_tag="OO150_000352"
     CDS             complement(4726..6174)
                     /gene="thiI"
                     /locus_tag="OO150_000352"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5514618.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIALQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            6380..6622
                     /gene="xseB"
                     /locus_tag="OO150_000353"
     CDS             6380..6622
                     /gene="xseB"
                     /locus_tag="OO150_000353"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5514619.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6622..7521
                     /gene="ispA"
                     /locus_tag="OO150_000354"
     CDS             6622..7521
                     /gene="ispA"
                     /locus_tag="OO150_000354"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5514620.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSPDTSALEALADYIIQRNK"
     gene            7546..9408
                     /gene="dxs"
                     /locus_tag="OO150_000355"
     CDS             7546..9408
                     /gene="dxs"
                     /locus_tag="OO150_000355"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5514621.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            9463..10437
                     /gene="yajO"
                     /locus_tag="OO150_000356"
     CDS             9463..10437
                     /gene="yajO"
                     /locus_tag="OO150_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5514622.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(10491..11009)
                     /gene="pgpA"
                     /locus_tag="OO150_000357"
     CDS             complement(10491..11009)
                     /gene="pgpA"
                     /locus_tag="OO150_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5514623.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10987..11964)
                     /gene="thiL"
                     /locus_tag="OO150_000358"
     CDS             complement(10987..11964)
                     /gene="thiL"
                     /locus_tag="OO150_000358"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5514624.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGVRIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(12042..12461)
                     /gene="nusB"
                     /locus_tag="OO150_000359"
     CDS             complement(12042..12461)
                     /gene="nusB"
                     /locus_tag="OO150_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5514625.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(12481..12951)
                     /gene="ribE"
                     /locus_tag="OO150_000360"
     CDS             complement(12481..12951)
                     /gene="ribE"
                     /locus_tag="OO150_000360"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5514626.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(13040..14143)
                     /gene="ribD"
                     /locus_tag="OO150_000361"
     CDS             complement(13040..14143)
                     /gene="ribD"
                     /locus_tag="OO150_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5514627.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWGEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(14147..14596)
                     /gene="nrdR"
                     /locus_tag="OO150_000362"
     CDS             complement(14147..14596)
                     /gene="nrdR"
                     /locus_tag="OO150_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5514628.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14747..15286
                     /locus_tag="OO150_000363"
     CDS             14747..15286
                     /locus_tag="OO150_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5514629.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15585..16469
                     /gene="tsx"
                     /locus_tag="OO150_000364"
     CDS             15585..16469
                     /gene="tsx"
                     /locus_tag="OO150_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5514630.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTDLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16646..16993)
                     /locus_tag="OO150_000365"
     CDS             complement(16646..16993)
                     /locus_tag="OO150_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5514631.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(17122..18093)
                     /gene="secF"
                     /locus_tag="OO150_000366"
     CDS             complement(17122..18093)
                     /gene="secF"
                     /locus_tag="OO150_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5514632.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGEDLTQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(18104..19951)
                     /gene="secD"
                     /locus_tag="OO150_000367"
     CDS             complement(18104..19951)
                     /gene="secD"
                     /locus_tag="OO150_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5514633.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19979..20311)
                     /gene="yajC"
                     /locus_tag="OO150_000368"
     CDS             complement(19979..20311)
                     /gene="yajC"
                     /locus_tag="OO150_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5514634.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(20334..21461)
                     /gene="tgt"
                     /locus_tag="OO150_000369"
     CDS             complement(20334..21461)
                     /gene="tgt"
                     /locus_tag="OO150_000369"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5514635.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(21517..22587)
                     /gene="queA"
                     /locus_tag="OO150_000370"
     CDS             complement(21517..22587)
                     /gene="queA"
                     /locus_tag="OO150_000370"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5514636.1"
                     /translation="MRVTDFFFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTSVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22680..23261
                     /gene="acpH"
                     /locus_tag="OO150_000371"
     CDS             22680..23261
                     /gene="acpH"
                     /locus_tag="OO150_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR5514637.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALGTRFWQFYPRMMAQASHKAL"
     gene            complement(23266..24988)
                     /gene="malZ"
                     /locus_tag="OO150_000372"
                     /pseudo
     CDS             complement(23266..24988)
                     /gene="malZ"
                     /locus_tag="OO150_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(25144..26517)
                     /gene="proY"
                     /locus_tag="OO150_000373"
     CDS             complement(25144..26517)
                     /gene="proY"
                     /locus_tag="OO150_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5514638.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(26593..27912)
                     /gene="brnQ"
                     /locus_tag="OO150_000374"
     CDS             complement(26593..27912)
                     /gene="brnQ"
                     /locus_tag="OO150_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5514639.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(28319..29614)
                     /gene="phoR"
                     /locus_tag="OO150_000375"
     CDS             complement(28319..29614)
                     /gene="phoR"
                     /locus_tag="OO150_000375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5514640.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(29672..30361)
                     /gene="phoB"
                     /locus_tag="OO150_000376"
     CDS             complement(29672..30361)
                     /gene="phoB"
                     /locus_tag="OO150_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5514641.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            30551..31753
                     /gene="sbcD"
                     /locus_tag="OO150_000377"
     CDS             30551..31753
                     /gene="sbcD"
                     /locus_tag="OO150_000377"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5514642.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALAESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31750..34893
                     /gene="sbcC"
                     /locus_tag="OO150_000378"
     CDS             31750..34893
                     /gene="sbcC"
                     /locus_tag="OO150_000378"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5514643.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQVSGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVKAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            35019..36251
                     /gene="araJ"
                     /locus_tag="OO150_000379"
     CDS             35019..36251
                     /gene="araJ"
                     /locus_tag="OO150_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5514644.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMFFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAGE
                     TTGVGYSLGVHYLLPDAA"
     gene            complement(36350..37260)
                     /gene="mak"
                     /locus_tag="OO150_000380"
                     /pseudo
     CDS             complement(36350..37260)
                     /gene="mak"
                     /locus_tag="OO150_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
     gene            37385..38296
                     /gene="rdgC"
                     /locus_tag="OO150_000381"
     CDS             37385..38296
                     /gene="rdgC"
                     /locus_tag="OO150_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5514645.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(38374..38457)
                     /locus_tag="OO150_000382"
     CDS             complement(38374..38457)
                     /locus_tag="OO150_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514646.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(38454..38795)
                     /locus_tag="OO150_000383"
                     /pseudo
     CDS             complement(38454..38795)
                     /locus_tag="OO150_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38943..39227)
                     /gene="ppnP"
                     /locus_tag="OO150_000384"
     CDS             complement(38943..39227)
                     /gene="ppnP"
                     /locus_tag="OO150_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5514647.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(39299..39976)
                     /locus_tag="OO150_000385"
     CDS             complement(39299..39976)
                     /locus_tag="OO150_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5514648.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(40234..40425)
                     /gene="yaiA"
                     /locus_tag="OO150_000386"
     CDS             complement(40234..40425)
                     /gene="yaiA"
                     /locus_tag="OO150_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5514649.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(40475..40999)
                     /gene="aroL"
                     /locus_tag="OO150_000387"
     CDS             complement(40475..40999)
                     /gene="aroL"
                     /locus_tag="OO150_000387"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5514650.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(41182..41640)
                     /locus_tag="OO150_000388"
     CDS             complement(41182..41640)
                     /locus_tag="OO150_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5514651.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            41760..42569
                     /gene="proC"
                     /locus_tag="OO150_000389"
     CDS             41760..42569
                     /gene="proC"
                     /locus_tag="OO150_000389"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5514652.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(42586..43701)
                     /gene="adrA"
                     /locus_tag="OO150_000390"
                     /gene_synonym="dgcC"
     CDS             complement(42586..43701)
                     /gene="adrA"
                     /locus_tag="OO150_000390"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5514653.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(43803..44123)
                     /gene="psiF"
                     /locus_tag="OO150_000391"
     CDS             complement(43803..44123)
                     /gene="psiF"
                     /locus_tag="OO150_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5514654.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(44242..45657)
                     /gene="phoA"
                     /locus_tag="OO150_000392"
     CDS             complement(44242..45657)
                     /gene="phoA"
                     /locus_tag="OO150_000392"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5514655.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(45758..46018)
                     /gene="iraP"
                     /locus_tag="OO150_000393"
     CDS             complement(45758..46018)
                     /gene="iraP"
                     /locus_tag="OO150_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5514656.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            complement(46245..46402)
                     /locus_tag="OO150_000394"
                     /pseudo
     CDS             complement(46245..46402)
                     /locus_tag="OO150_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            46480..47574
                     /gene="ddlA"
                     /locus_tag="OO150_000395"
     CDS             46480..47574
                     /gene="ddlA"
                     /locus_tag="OO150_000395"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5514657.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLRLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(47598..47810)
                     /locus_tag="OO150_000396"
     CDS             complement(47598..47810)
                     /locus_tag="OO150_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5514658.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGAVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            48070..48378
                     /locus_tag="OO150_000397"
     CDS             48070..48378
                     /locus_tag="OO150_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5514659.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(48437..49531)
                     /gene="yaiW"
                     /locus_tag="OO150_000398"
     CDS             complement(48437..49531)
                     /gene="yaiW"
                     /locus_tag="OO150_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5514660.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(49544..50764)
                     /gene="sbmA"
                     /locus_tag="OO150_000399"
     CDS             complement(49544..50764)
                     /gene="sbmA"
                     /locus_tag="OO150_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5514661.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            51116..52273
                     /gene="ampH"
                     /locus_tag="OO150_000400"
     CDS             51116..52273
                     /gene="ampH"
                     /locus_tag="OO150_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5514662.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(52274..52897)
                     /gene="iprA"
                     /locus_tag="OO150_000401"
     CDS             complement(52274..52897)
                     /gene="iprA"
                     /locus_tag="OO150_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR5514663.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FFILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(52985..>54608)
                     /locus_tag="OO150_000402"
     CDS             complement(52985..>54608)
                     /locus_tag="OO150_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5514664.1"
                     /translation="NNGSQVNVTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSY
                     STVNLTESTGWNNYSNLYTNTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHN
                     GNVISGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNG
                     TELDNSSVDNVVAATGNYKVRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKAD
                     LGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGL
                     ADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGD
                     MNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNS
                     DAWGFGLKAGYDFKLGDAGYETPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELG
                     VDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSF
                     TKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT        13508 a        14482 c        13644 g        12974 t
ORIGIN      
        1 cactaagagc ggcggttatg atgtttaagc aatacctgca agtaacgaaa ccaggcatca
       61 tctttggcaa cctgatctcg gtgattgggg gattcctgct ggcctcaaag ggcagcattg
      121 attatcccct gtttatctac acgctggttg gggtgtcact ggttgtggcg tcgggttgtg
      181 tgtttaacaa ctacatcgac agggatatcg acagaaagat ggaaaggacg aagaatcggg
      241 tgctggttaa aggcctgatc tctcctgctg tctcgctggt gtacgccacc ttgctgggta
      301 ttgctggctt tatgctgctg tggtttggcg cgaatccgct ggcctgctgg ctgggggtga
      361 tgggctttgt ggtttatgtc ggcgtctata gcctgtacat gaaacgccac tcagtctacg
      421 gcacattgat tggttcgctc tccggcgctg cgccgccggt gattggctac tgtgcagtaa
      481 ccggcgagtt cgatagcggc gcagcgatcc tgctggctat cttcagcctg tggcagatgc
      541 ctcactccta tgccatcgcc attttccgct ttaaggatta ccaggcggca aacattccgg
      601 tattgccagt ggtaaaaggc atttcggtgg cgaagaatca catcacgctg tatatcatcg
      661 cctttgccgt tgccacgctg atgctctctc ttggcggtta cgctggatat aaatatctgg
      721 tggtcgccgc ggcggttagc gtctggtggt taggtatggc tctgcgcggt tataaagttg
      781 ctgatgacag aatctgggcg cgcaagctgt tcggcttctc tatcatcgcc atcactgccc
      841 tctcggtgat gatgtccgtt gattttatgg taccggactc gcatacgctg ctggctgctg
      901 tgtggtaaca aaacctctct attaaaaagg tgctacggca ccttttttct tagcattaga
      961 aacatatccc tctcgaaata tttactaaaa aatccgcatg tttaccccat tcgtttgccg
     1021 ctttacacta gtcgcgaatt taaaacagag gtggtaatga acgattataa aatgacgcca
     1081 ggtgagaggc gcgcgacctg gggtttaggg accgtattct cgttgcgcat gctgggcatg
     1141 tttatggttc tgccggttct gaccacgtat ggcatggctc tgcaaggtgc cagcgaagca
     1201 ttaatcggta ttgccattgg tatttatggt ctgactcagg ccgtttttca gattccgttt
     1261 ggcctgcttt ccgaccgtat tggtcgcaaa ccattaattg tcggtgggct ggcggtgttt
     1321 gccgccggta gcgttatcgc tgcgctctct gactccatct ggggaattat tctaggccgg
     1381 gcgctacaag gctccggtgc gattgccgct gccgttatgg cgctgctttc cgatctcacg
     1441 cgcgaacaaa accgcaccaa agcaatggcg tttatcggcg tgagctttgg cattaccttt
     1501 gccattgcga tggtgcttgg cccgatcatc actcacaaac ttgggctgca cgcgctgttc
     1561 tggatgatcg ctattctggc aacgaccggc attgcgttga ccatttgggt tgtgcccaac
     1621 agtagcactc acgtacttaa tcgtgagtcc ggaatggtga aaggcagttt cagtaaagtg
     1681 ctggcggaac cgcggctgct gaaactcaac tttggcatta tgtgtctgca tattttgctg
     1741 atgtcgacgt ttgttgccct gcccggacaa ctggctgatg cagggttccc ggcggctgaa
     1801 cactggaagg tctatctggc gacaatgcta atcgcctttg gctcggtcgt gcctttcatt
     1861 atctacgctg aagttaagcg caaaatgaag caagtctttg tcttctgcgt cggattgatc
     1921 gtggttgcgg aaattgtgtt gtggaacgcg caaacgcagt tctggcaact ggtggtcggc
     1981 gtgcagcttt tctttgtagc gtttaatttg atggaagccc ttctgccttc acttatcagt
     2041 aaagagtcgc cagcaggtta caaaggtaca gcgatgggtg tttactccac cagccagttt
     2101 cttggcgtgg cgattggcgg ttcactgggc ggctggattg acggcatgtt tgacggtcag
     2161 ggcgtatttc tcgctggcgc aatgctggcc gcagtgtggc tggcagtcgc cagtaccatg
     2221 aaagaaccgg cgtatgtcag cagtttgcgc attgaaatcc cggcgaacat tgccgcaaac
     2281 gaggcgttaa aagtgcgttt gctagaaact gaaggcatca aagaagtgtt gattgcagaa
     2341 gaagaacatt cagcttatgt gaaaatcgac agcaaagtga cgaatcgctt tgatgttgaa
     2401 caggcaattc gccaggcata aaagcgaggc gaaattgcct gctgcgctac gctcatcagg
     2461 cctactaagc tcctgattta caagcctttg taggccgaac caggcgttca tgccgcatct
     2521 gacatgaaca aagcgcaggt tgtcgcgatt aatcgcggaa gtttttgaac tggaacggct
     2581 gacccagatc gccaccgcgc accatcgcca tgacagcctg caaatcatca cgagatttgc
     2641 cagtcacgcg gatctcatcg ccctgaattt gcgcctgcac tttcagtttg ctgtctttga
     2701 tcatcttgac gatttttttc tgggtcgcgc tctcaatgcc ctgcttcagt ttcgcttcca
     2761 caaaccaggt tttaccgcta tgaacgatat tttccggtac atccagtgaa ctgccttcaa
     2821 tgccgcgctt cagcagcttg gcacgcagaa tatccagcaa ctgattgacc tggaaatcgg
     2881 actcgctcaa cactttgatg gttttgctgg cgtcgttcag ctcaaatgag gcttcaacgt
     2941 tacggaagtc aaaacgggac tccacttcgc ggctcgcgtt atcgaccgcg ttacgtgctt
     3001 cctgaagatc aacttcagag acaatatcga aagatggcat cttttcctct cccttcattt
     3061 ttgatgcgaa gcataatacc cgcaaagtta aataaccgac agtcctgcac caggacgtat
     3121 cccttaattg tgcatagtca caacagcatg cacaattaag ggatagcctt attgaacacc
     3181 cggagtggtt gcgggtgagg aggaacaatg aaaattaccg tattgggatg cggtgcctta
     3241 gggcaattat ggcttacagc actttgcaaa cagggtcatg aagttcaggg ctggctgcgc
     3301 gtaccgcaac cttattgtag tgcgaatctg gttgagacag atggttcgat atttaacgaa
     3361 tcgctgaccg ccaacgatcc cgattttctc gccaccagcg atctgctcct ggtgacgctg
     3421 aaagcatggc aggtttccga tgccgtcaaa agcctcgcgt ccacactgcc tgtaaccacg
     3481 ccaatactgt taattcacaa cggcatgggc accatcgaag agttgcaaaa tattcagcag
     3541 ccattactga tgggcaccac cacccatgcc gcccgccgcg acggcaatgt cattattcat
     3601 gtggcaaacg gtatcacgca tattggtccg gcacggcaac aggatggcga ttacagttat
     3661 ctggcggata ttttacaaac cgtgttgcct gacgttgcct ggcataacaa tattcgcgcc
     3721 gagctgtggc gcaagctggc agtcaactgc gtgattaatc cactgaccgc catctggaat
     3781 tgcccgaacg gtgaattacg tcatcatccg caagaaatta tgcaaatatg cgaagaagtc
     3841 gcggcagtga tcgaacgcga agggcatcat acttcagcgg aagatttgcg tgattacgtg
     3901 atgcaggtga ttgatgccac agcggaaaat atctcgtcga tgttgcagga tatccgcgcg
     3961 ctgcgccaca ccgaaatcga ctatatcaat ggttttctct tacgccgcgc ccgcgcgcat
     4021 gggattgccg taccggaaaa cacccgcctg tttgaaatgg taaaaagaaa ggagagtgaa
     4081 tatgagcgca tcggcactgg tttgcctcgc ccctggtagt gaagagactg aagccgtcac
     4141 cacgatcgac ctcttggttc gcggcggtat caaagtcacc actgccagcg tagccagcga
     4201 tggcaacctg gcaattacct gctcgcgcgg cgtgaagctg ctggcggatg cgccgctggt
     4261 cgaggtggct gatggcgaat atgacgtgat cgtactgcct ggtggcatta aaggcgcaga
     4321 gtgttttcgc gatagcactc tgctggttga aaccgttaaa cagttccacc gttccgggcg
     4381 tatcgtcgcg gctatttgcg ccgcgccagc caccgtgctg gtgccgcacg atatcttccc
     4441 gattggtaat atgaccggct tcccgacgct gaaagacaaa attcccgctg aacaatggca
     4501 ggacaagcgc gtcgtctggg atgcacgggt aaaattgctg accagccagg ggccggggac
     4561 agctatcgac tttggtctga aaattatcga cctgcaggtt gggcgcgaga aagcccatga
     4621 agtggcatca caactggtga tggcggcagg gatttataat tattacgagt agtgtcggat
     4681 gcggtaagcg tcgcatctga ccagatgcga cgttacaacc acaaattacg ggcgatatac
     4741 cttcacattg ttaaagccct gctcgcgcag atagagcgcc tgcagacggc tcatcacccc
     4801 gcgctcacac cacagtagcc aggttctgtt ctggtcgaga tcgccaaact tggtgctcag
     4861 tttatagaac ggcagagaaa ctacatcaat cccttcgact ttcagtggct tatcttcctg
     4921 ttcatcgata gaacggatat cgaggatcac gtcgttcggg ccgaagccat tgacggtttc
     4981 cacttccacc acttcctgct cggtctgcag ggcgatttcg cggatatcaa cgttattcgc
     5041 ttcttcaacc actttatcga gaatgctgaa gtcgaatttc tcttcttccg cttcaatctt
     5101 cgacttaacc gctttcaccg tcgggctttt ggagataaca ccgcaatatt ccggcatcgt
     5161 gcgagcaaag tcttcggtgc caatctggcg agccaggttg atgatgtgct ctttgtcgta
     5221 agaaatcagc ggacgcagga tcagcgtatc ggagacgtta tcaatcaggc gcaggttggt
     5281 cagcgtctga ctggacacct ggccgagcgc ttcgccggtg accagcgcct gtacgccgta
     5341 acgttcagcc actttagacg cggcacgcac catcatacgt ttgaggataa cgcccatctg
     5401 accgtcgtcg attttctcga gaatttcccc gacgaccggt tcgaaattaa tagcgacaaa
     5461 acgcacgcgg tgggagctgc caaaacggtt ccacagataa tgcgccacct gacgcacgcc
     5521 aatttcatgc gccgcgccgc caaggttaaa gaaacagtaa tgcacgcggc agccgcgacg
     5581 catcaacata taactggaaa caccggagtc gaaaccaccg gaaatgagcg acagcacatc
     5641 ttcctgggtg ccgatcggga aaccgccaat accttcgtag cggcctttaa tcagcagaag
     5701 acgatcgtct tccacttcca gatggacagt cacttccgga ttggtcagct tcacgcgcgc
     5761 ggactcaata tgctgattta aaccgccgcc gacgtaacgt tccacatcaa tcgagctaaa
     5821 atcatgtttg ccacggcgct tcacgcgtac gcagaaggtt ttgccttcca gctgatcgcg
     5881 atactgaacc aacgctttct caaaaatatc gtgcatgtcg gtaaacggca cgtcttcgac
     5941 ttcgagaata tggtggatac ccggaatacg ggtcagggcg tcgcgaatag ccagacgctg
     6001 gttttcatct tttgcacgaa cttcgatgtt atcccagtgg cggacgacag cgagcgtctc
     6061 atcatagtgc tttaaaacgt tacgaatgtt cccggtaagg atttttataa agcgcaagcg
     6121 cacagattgg cttttgatgg tgatttccgg gaacaattta atgataaact tcatggcggc
     6181 aatggttcgt tggcaagcct taagcgactt gtatagggaa aaatacagca gcccacacct
     6241 gcggctgcat ccaggcgcgg aagtatacca ctaacatcgc tttgctgcgc acatcacctt
     6301 accattgcgc gttatttgct atttgccctg agtccgttac catgacgggg caaaaaatat
     6361 tgagagtcag acattcatta tgccgaagaa aaatgaggct cccgccagct ttgaaaaggc
     6421 gctgagcgag ctggaacaga ttgtaacccg tctggaaagt ggcgacctgc cgctggaaga
     6481 ggcgctgaac gagttcgaac gcggcgtgca gctggcacgt caggggcagg ccaaattaca
     6541 acaagccgaa cagcgcgtac aaattctgct gtctgacaat gaagacgcct ctctaacccc
     6601 ttttacaccg gacaatgagt aatggacttt ccgcagcaac tcgaagcctg cgttaagcag
     6661 gccaaccagg cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc
     6721 gaaaccatgc agtatggtgc attattaggt ggtaagcgtc tgcgaccctt cctggtttat
     6781 gccaccggtc atatgtttgg cgttagcaca aacacgctgg acgcacccgc tgccgccgta
     6841 gagtgtatcc acgcttactc attaattcat gatgatttac cggcgatgga tgatgacgat
     6901 ctgcgtcgcg gtttgccgac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct
     6961 ggcgacgctt tacaaacgct ggcgttctcg attttaagcg atgctgatat gccggaagtg
     7021 tcggatcgcg acagaatttc gatgatttct gaactggcga gcgccagcgg tattgccgga
     7081 atgtgcggtg gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg
     7141 cttgagcgta ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt
     7201 gcattaagcg ccggagataa agggcgtcgt gctctgccag tactcgacaa gtacgcagag
     7261 agcatcggcc ttgccttcca ggttcaggat gacatcctgg atgtggtagg agatactgca
     7321 acgttgggaa aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt
     7381 ctgggtcttg agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgtcagtcg
     7441 ctgaaacaac tggctgaaca gtcacccgat acctcggcac tggaagcgct agcggactac
     7501 atcatccagc gtaataaata aacaataagt attaataggc ccctgatgag ttttgatatt
     7561 gccaaatacc cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa
     7621 gagagtttac cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt
     7681 tccagcgggc acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat
     7741 gtctacaaca ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc ttatccgcat
     7801 aaaattttga ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggcctgcac
     7861 ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc
     7921 tccatcagtg ccggaattgg tattgcggtt gctgccgaga aagaaggcaa aaatcgccgt
     7981 accgtctgtg taatcggcga tggcgcgatt accgcaggca tggcgtttga agcgatgaat
     8041 cacgcgggcg atatccgtcc tgatatgctg gtggtcctca acgacaacga aatgtcgatt
     8101 tccgaaaatg tcggcgcgct caacaaccat ctggcgcagc tgctttccgg taagctttac
     8161 tcttcactgc gcgaaggcgg gaaaaaagtt ttctctggcg tgccacccat taaagagctg
     8221 ctcaaacgta ccgaagaaca tattaaaggc atggtagtcc ctggcacgtt gtttgaagag
     8281 ctgggcttta actacatcgg cccggttgac ggtcacgatg tgctggggct tatcaccacg
     8341 ctgaagaaca tgcgtgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt
     8401 cgtggttatg aaccggcaga aaaagaccca atcactttcc acgccgtgcc taaatttgat
     8461 ccctccagcg gttgtttgcc gaaaagtagc ggcggtttac cgagctattc aaaaatcttt
     8521 ggcgactggt tgtgcgaaac ggcggcgaaa gataacaagc tgatggcgat tactccggcg
     8581 atgcgtgaag gttccggcat ggtcgagttt tctcgtaaat tcccggatcg ctacttcgac
     8641 gtggcaattg ccgagcaaca cgcagtgacc tttgccgccg gtctggcgat tggtgggtac
     8701 aaacccattg tcgcgatcta ctccactttc ctgcaacgcg cctatgatca ggtgctgcat
     8761 gacgtggcaa ttcaaaagct tccggtcctg ttcgccatcg accgcgcggg cattgttggt
     8821 gctgacggtc aaacccatca gggtgctttt gatctctctt acctgcgctg cataccggaa
     8881 atggtcatta tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta taccggctat
     8941 cactataacg atggcccgtc agcggtgcgc tacccgcgtg gcaacgcggt cggcgtggaa
     9001 ctgacgccgc tggaaaaact accaattggc aaaggcattg tgaagcgtcg tggcgagaaa
     9061 ctggcgatcc ttaactttgg tacgctgatg ccagaagcgg cgaaagtcgc cgaatcgctg
     9121 aacgccacgc tggtcgatat gcgttttgtg aaaccgcttg atgaaacgtt aattctggaa
     9181 atggccgcca gtcatgaagc actggttacc gtagaagaaa acgccattat gggcggtgca
     9241 ggcagcggag tgaacgaagt gctaatggcc catcgtaaac cagtccccgt gctgaacatt
     9301 ggcctgcctg acttctttat tccgcaagga acgcaggaag aaatgcgcgc cgaactcggc
     9361 ctcgatgccg ccggtatgga agccaaaatc aaggcctggc tggcataatc cctactccac
     9421 tcctgctatg cttaagaaat tattcatggc aggagtggca gcatgcaata caacccctta
     9481 ggaaaaaccg accttcgcgt ttcccgactt tgcctcggct gtatgacctt tggcgagcca
     9541 gatcgtggta atcacgcatg gacactgccg gaagagagca gccgtcccat aattaaacgc
     9601 gcactggaag gcggcataaa tttctttgat accgccaata gctattccga tggtagcagc
     9661 gaagagatcg ttggtcgcgc actgcgggat ttcgcccgtc gtgaagacgt ggtcgttgcc
     9721 accaaagtgt tccatcgcgt tggtgattta ccggaaggat tatcccgtgc acaaattttg
     9781 cgctctatcg acgacagcct gcgacgtctc ggcatggatt atgtcgatat cctgcaaatt
     9841 catcgctggg attacaacac gccgatcgaa gagacgctgg aagccctgaa cgacgtggta
     9901 aaagccggga aagcgcgtta tatcggcgcg tcatcaatgc acgcttcgca gtttgctcag
     9961 gcactggaac tccaaaaaca gcacggctgg gcgcagtttg tcagtatgca ggatcactac
    10021 aatctgattt atcgcgaaga agagcgcgag atgctgccac tgtgttatca ggagggcgtg
    10081 gcggtgattc cgtggagccc gctggcgcgg gggcgactga cgcgtccgtg gggagaaact
    10141 accgcacgac tggtgtctga tgaggtgggg aaaaatctct ataaagaaag cgatgaaaat
    10201 gacgcgcaga tcgcagagcg gttaacgggc gtcagtgaag aactcggtgc aacacgagca
    10261 caagttgcgc tggcttggtt gttgagtaaa ccgggcattg ccgcaccgat tatcggtaca
    10321 tcgcgggaag aacagcttga tgagctattg aacgcggtgg atatcacttt gaagccggaa
    10381 cagattgccg aactggaaac gccgtataaa ccgcatccgg tagtaggatt taaataagag
    10441 tccaggcctg atgagacgca acaagcgtca catcaggcat cggtgcacaa ctacgacaga
    10501 atgcccagcg gccagtgatg accaataaag tacaggatgc cagcggagat caccccggcg
    10561 acaatatcat ctatcatgat ccccatgccg ccatgcacat tgcgatcaaa ccagcggatc
    10621 ggccacggtt tccacatatc cagaatacgg aaaatcacaa acccggcggc aacccactgc
    10681 cagtcattgg tcggcagcgc catgagcgtg atccacatac caataaattc gtcccagaca
    10741 atgctgccgt gatcgtgcac gcccatatct tttgcggtct gatgacaaag atagacgccg
    10801 atacagatcc ccagcatcac caccagcgag tagagctgcc agggcaaaaa ggtcatcaga
    10861 taccagaacg gaatcgctgc cagcgagccc atcgtcccag gaacgatcgg gcttaatcca
    10921 cttccgaatc cgacagcaag tagatgccac ggattactca tcttcaggcg acttttcgcg
    10981 acatctttat ggcgtggcaa aatggtcata tcctttccag tctaacgtaa caggttcacc
    11041 gtcacgaata aaacaaagcc cttcgatatc ggcggtcatt tgcccgatac aggtaaacgg
    11101 tacgcccagg tgtccgagag ccacatccag cgctccacgg ttcagttccg gcaccgtgaa
    11161 acacaactcg taatcttcac cgccagagag cgcccagcgc agcgcctgtt ccggttcaac
    11221 atggcgagaa agcgcatcag aaaacggcag caatgccagg tcaatacgta cgccgcagtc
    11281 gctggctttc acgatatgcc cgagatcgga aatcagaccg tcagagagat cgatagctga
    11341 atttgccaga tcgcgcagtg cctgcccctg taaaatacgc ggcgatggac ggagatgacg
    11401 tttgatcaag tagtccgcat ctttagcatc ggcaacctgc aaacggtttt gcaaaatcgc
    11461 cagcccggcg gcgctatcgc ccggtgtacc ggtcacatag atccagtcac ccggtttcgc
    11521 cccagagcgc gttaaggctc gtcccatcgg aacaaagccg tggataccca acgtcattga
    11581 taatggccca cgcgtggtat cgccgccaat gagttgcata tcgtaataat tgagaagatc
    11641 aaacaaactg tcgctgaagg actcaagcca cgcttcgtct acgtccggca aggttaatgc
    11701 cagcgtcagc caggccggat cggcccccat cgctgccaga tcgcttaggt tcaccgccag
    11761 tgctttatac gccagatcag caggatcgat atcagggagg aagtggttgc ccgccaccag
    11821 cgtatcagtg ctgatcgcca gggtctgttt ctcggggata ttgagaagtg cgcaatcgtc
    11881 gccgatgccc agttcgacat caagacgaga acttcttaca cggtcaaaat aacgggcaat
    11941 cagggagaac tcgccacatg ccatacgtta tgcctcagca ggtaaaaaag aaaaggccgg
    12001 cgatcatgga acggtcttcc gtgaatctac cggcctggat attacttttt gttagggcga
    12061 atcacaggtg ctgctttatc gagtacaccg ttgacgaact tatggctgtc ttctgcgccg
    12121 aacgatttcg ccagttcgat cgcttcgtta atggccactt tgtatggcac atcgctacgt
    12181 ttagacagtt cgtacagcgc aatgcgcagt actgcttttt ctacctgtcc cagttcttcc
    12241 agcagacggg acaggtatgg cttcatcagt ccgtcgaggt atgcggtatt agtcgccacc
    12301 ccggccagca gctcacggaa gtacaggacg tcaacgtctt ttacatcctg ttcagccagg
    12361 aactggtatt caacatcagc gatgtcgttc tgggacaact gccaggagta gagcgcctgg
    12421 acggcacact cacgagcgcg gcgacgagca gcaggtttca cggatttccc cttactaatt
    12481 tcaggccttg atggctttca atacattaat catttcaagc gcggtcagtg cagcttctgc
    12541 acctttgttg ccagctttgg tgccagcacg ttcgatcgct tgttcaatgc tttcagtggt
    12601 cagaacccca aaagcaaccg gaatttcgct gtcctgggca acatgcgcca ggccgttgct
    12661 tgcaccacca gcgacatatt caaaatgggc agtgccacca cgaataaccg tacccagcgc
    12721 aatcaccgcg tcgtatttac cggttttagc cagtgcaccc gccgccagcg gcagctcata
    12781 ggcaccaggc acccaaacaa cggtaatgtt ttcatctttt acctgaccga tacgtttcag
    12841 tgcgtcaatt gcaccttcca gcaggctgtc attgataaag ttgttgaaac gcgcgatggt
    12901 gatggcgacg cgagcgtccg gggtagcaac gttagcttca ataatgttca ttttcttcct
    12961 tcgggttcga gtatggcccc gcaggggggc ggattttagc ataatatttc gtgcgctgct
    13021 tccctttcga gccgggagat catgcaccca ctaaatgcag gcaaacatcc gggcctacat
    13081 gacgtatctc tttgaattta aattgggggg cgtcggctaa tttctcaagc cctggcaggg
    13141 tgcataatcc acgggcgtcg ctgcctaata gtttaggtgc gatatagaca atcagctcat
    13201 cgactaaacc cgcctgcagc aatgcgccag cgagcgttgg ccccgcttcc ccccagatgc
    13261 tgttaatctg ctgtttaccc agttgcatca tcagtacaac cagatccaga tgacctttat
    13321 gctctggaat cagcaaggta cgcaccgttt ccggccactc acgagaatct tcctgcgtac
    13381 gcgcgaacca ggtttcgccg ggctgctgca caatgtgatg ttccggcgtc acgcgatttt
    13441 ggctatcaat cacaatacgt accggctggc ggagattttg ttgcgggtag agcgcctgag
    13501 tttgttcatc cagttcggac caacgcaccg ttaaggcagg atcatccgcc agcaccgtgg
    13561 cgctgctggt taaaatggca tgactttgcg cgcgcagtcg ttgtacatca cgccgcgcct
    13621 ggggcgaagt gatccactgg ctttcgccgc tcgccatcgc cgtgcgacca tcaagcgatg
    13681 cgccaagttt taactgaata taaggaaagc cggtgcgcat ccgcttgaga aagcctttat
    13741 tcaattgctc ggcttcactc atcatcaggc cgtggctgac gtcaatgcca gcctgttgca
    13801 gacggtaaag tccacgccca gcgacctgcg ggttaggatc ttgcatcgag gcaaccacgc
    13861 gcgccacacc tgcggcaatt aaagcatcac agcaaggcgg cgtacggccg tgatggctgc
    13921 aaggttcgag tgtcacatac gccgttgcac ctttggcttt ttcacccgcc atacgcaacg
    13981 cgtgtacttc ggcatgtggt tcacccgctc gttgatggta accttcaccg acaatttcgc
    14041 catctttgac aatgacgcac ccgacatttg ggttggggtg ggtggtaaaa cgtcctcgtt
    14101 gcgccagctt tagcgcccgc gccatgtaat actcgtcctg cacggcttag tcctccaggc
    14161 gcgcgatctc ttcgccaaat tctttgatat cttcgaaact gcggtagaca gaggcaaaac
    14221 ggatataggc gactttatcg agctttttca attgctccat caccagattg ccaatcatct
    14281 tgctcggtac ttcgcgctca ccggtggcgc gcagctgcga tttaatatga ttgattgcca
    14341 tttcaacgtc atcggaactc accggacgtt tttccagcgc ccgcagcatt ccgctacgca
    14401 atttctcttc attaaacggt tcacgcacgt cgttgctttt tacaacacgc ggcataacca
    14461 gctccgccac ttcaaaggtg gtgaaacgtt cattacacac cagacactgc cgacggcggc
    14521 gtacggatga accctcgccc acgagacgag agtcaattac cttagtgtcc acggcgaaac
    14581 agaatgggca atgcatacgg cgtcctgacc aagtggttaa cggaaatcta ttttaccctg
    14641 aactgacgat acaacaaagg cgcaacgctt ttgagataaa ggatcgatgc tttcgctgct
    14701 tttaccctct accattaggc taattgcgac gagaaaggga cgacctatga atactaacgt
    14761 ttttcgactg ctcctgctgg gaagcctgtt cagccttagc gcctgtgtgc agcaaagtga
    14821 agtgcgacag atgaaacaca gcgtcagcac gctgaaccag gagatgacgc aactcaatca
    14881 agaaacagtc aaaatcactc agcaaaacag gctgaatgca aaatccagca gtgggggtta
    14941 ccttctgcct ggagcgaaaa caccggcaag actggaaagc cagatcggta ctttacgtat
    15001 gtcgctggtg aatattacgc ctgatacaga tggcaccacc cttacactac gtattcaggg
    15061 tgagtcgaat gatcccttgc ccgcattcag cggaactgtt gaatatgggc aaattcaggg
    15121 aacaatagac aactttcagg aaatcaatgt gcagaatcaa ttgattaatg cccctgccag
    15181 cgtcctcgcc cccagcgatg ttgatattcc gttacaatta aaggggattt ctgtggatca
    15241 gttaggcttt gtacgtatcc acgacattca acctgttatg cagtaaacgt atttcgggac
    15301 gattttgtgc gtcccgcaac atctttcccc gtcattttgt tactctgctt acatcacctg
    15361 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    15421 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    15481 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    15541 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    15601 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    15661 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    15721 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    15781 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    15841 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    15901 tctccatcga caagctgacc aatactgacc ttaacttcgg tccgttcaaa gagtggtact
    15961 tcgcgaacaa ctacatttac gacatgggtc gtaataaaaa tggtcgccag agcacctggt
    16021 acatgggtct gggtaccgac atcgatactg atctgccgat gagcctgtcc atgaacgtct
    16081 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    16141 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    16201 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    16261 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    16321 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    16381 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    16441 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    16501 ttctgattgt taacaacgtg agctttgttc atgccggatg cggcgtaaac gctttgtccg
    16561 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    16621 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    16681 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    16741 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    16801 aaagcaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    16861 gaacggtaag ttcacgcagg ttggaataaa caaactcgcg ggaacagcga ccgcagaccc
    16921 acggatatat ttttaatgct ttttcgcgat agccgctttc taaccgcgca tagttttttg
    16981 ggatgatagc catgaagagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    17041 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    17101 tcaacatcaa cgggaacttg attacggcag aattgacggc tgatccgccc cttctttttc
    17161 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    17221 cacatagatg gaagatgcag taccgatgga aacaccgata agcatggtca gcgagaagcc
    17281 ttccagtacc ggaccaccga agaggtacag catcaggata accatcaagg tagtaccgga
    17341 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    17401 accgcgacgg atcttgcgga agttttcacg aatacggtcc gataccacga tactgtcgtt
    17461 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    17521 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gccagcgcaa taacaacccc
    17581 tgccgccagt cgccactcaa agcgaaaacc tacgtacaca aggatagaca gcagcgccgc
    17641 catcaacgcc atcgcaccgg tttgcgtaag gtcttccccc acgctcggac cgacgaactc
    17701 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacctggct
    17761 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    17821 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    17881 cattacgtca atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa
    17941 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcggcga ttaacagcag
    18001 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    18061 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    18121 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    18181 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    18241 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    18301 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    18361 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgccaccgca agggttaaga
    18421 cgatacccgc gatacctggc atactcagcg ttgcgcctgg caacagcgac ataatgccga
    18481 cgattaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    18541 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgtt
    18601 caatgttctg catacccagg gttgggccaa tggtacgttc ttcaacaatc tgaatcggcg
    18661 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    18721 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    18781 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    18841 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt agtgaagtta gacatgatgt
    18901 taccaccagc gctatcgagc gagatgttaa cctgcggttg gttgtattcg tcctggctgg
    18961 aagtggagtc ggtgatatgg tcaccggtca gaattacgcg tttgtacagc acaactggct
    19021 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    19081 cctggtcaac gttggtgttt accagacgga attccagcgt tgccgtcgca cccagaatct
    19141 ctttcgcacg cgcagtgtcc tgaatacctg gcagttcaac aacgatacgg tcagcaccct
    19201 gacgctgaac caccggctcc gctacgccaa gttggtttac acggttacgc aggatattga
    19261 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    19321 gcagctggtt gctgccctgg ctactaatca ccaggtccgg atggcgcttg ctcagatacg
    19381 caatggcttc atcacgagct ttagcatcgc ggaaagtaat gctcaggccg tagttgtttt
    19441 ctttacgaac ggtggtatac gggatgccct tttcacgcag atcgctgcgc aggctatcga
    19501 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    19561 cgccgccacg caggtcaagg ccgagcttca tcggctcagc gtgaatagct gccagccagc
    19621 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    19681 atgcttcacg agcgcgcagc tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    19741 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    19801 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    19861 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    19921 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    19981 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    20041 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    20101 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    20161 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    20221 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    20281 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    20341 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    20401 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    20461 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcaag atgatgcaag
    20521 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    20581 tcgctcttat acttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    20641 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccacg acgtacgcct
    20701 tcaaccaggt cttctggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    20761 tgcgggcata catgctccag aatgcggtgc atatctgctt tcggctcacc cacagccaga
    20821 ccgccgacag cgtagccatc aaaaccgata tctaccagac ctttaacaga aatatcacgt
    20881 aaatcttcgt aaacgctgcc ctgaatgata ccaaacagcg catttttgtt tccgagactg
    20941 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccatgga gcgttttgcg
    21001 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    21061 agatcgtact gaatctccat tgatttttca ggatcgagga aaatcggatc gccgttgatc
    21121 gggttacgga agtgcacgcc ctgttcggtg attttacgaa tatcgccaag gctgaagacc
    21181 tggaagcccc cggagtcggt gaggatcggc cccttccact gcataaaatc gtgcagatcg
    21241 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    21301 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    21361 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    21421 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    21481 aaaaacagtc caacgtttta aaccagcgcc gcggaattac tccccgacgc gctcattaat
    21541 tgcctgcgga ttgtacgtaa taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc
    21601 ttctaccgct gctttatagg cgttcatggt gtgttgataa ccggcaaagg ccgaaaccag
    21661 cataatcagc gtcgactctg gcaagtggaa gttcgtcacc agcgcatcga ccactttgta
    21721 ctggaagccc ggatagataa agatttgggt atcgtcgaag aacggttcaa tgagatcgtt
    21781 tttcgctgcc tgagccgcgc tttccagcga acgtactgaa gtggtgccaa ccgcaatcac
    21841 ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg gtacttcagc
    21901 gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc
    21961 cgcaccaacg tgcaacgtca caaacgccat ctccacgcct ttggcgcgca atttttccag
    22021 caaaggctcg tcaaaatgca gaccagcggt cggggctgca accgcgcccg gtttttcgct
    22081 ataaacggtt tgataaagtt cgcggtcagc gtcttcgtcc ggacggtcga tatacggcgg
    22141 cagcggcata tgcccgatac tgttgaggat atccagtacc gaacgatcat cattgaattc
    22201 gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg ttaatacttt cgtcatcgcc
    22261 cagcagcagt tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg
    22321 tttgtcgtcg agcatccgct caaccagcac ttcaatcttg ccgccgctgg ctttacgccc
    22381 aaacaggcgc gccgggatca cgctagtatt attaaaaacc agaagatcgc cggggttgag
    22441 cttatcgagt aaatcggtga aagtaccgtg cgtcagcgcg cccgtaggcc cgtccagcga
    22501 cagtaaacga cagctactgc gttcaggcat ggggtagtgg gcaatcaggg attcgggcaa
    22561 ttcaaagaag aaatcggtaa cgcgcatgac actgactcag actaaaataa gaggcgggta
    22621 gtctagtgcc ggggcgctct ccctgcaacc tttacccccc tttggataga atataatcga
    22681 tgaatttttt agctcacctg catttagccc atctcgcgga aagctcgctt tccggcaatt
    22741 tactggctga tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg
    22801 gcattcatat gcatcgacgt atcgacgtat tgactgacaa tctgccggaa gtccgcgaag
    22861 cacgggagtg gtttcgtcgt gaaacgcgcc gcgttgcgcc tattacgctg gatgtcatgt
    22921 gggatcactt tctttcccgc cactggtcgc agctgtcgcc ggactttccg ctacaggaat
    22981 ttatctgtta tgcccgcaag caagtgatga cgattttgcc ggactcaccg ccacgtttta
    23041 tcaatctaaa caattacttg tggtcagagc agtggctggt gcgctatcgc gatatggatt
    23101 tcatccagaa cgtgttaaac ggcatggcaa gccgtcgccc acgtctggat gccctgcgtg
    23161 actcctggta cgatttaaac gcccattata ccgccctcgg aacccgcttc tggcagtttt
    23221 atccgcggat gatggcgcag gcgtcacaca aggcgttata aaacgttagt tcatccatac
    23281 cgtagccgaa atggcaggca aagccagaat cccgtcagtc agttgcccat gcccttcttt
    23341 gcattgccat tgcacggcat tgagaaacgg tgacgcgggt agcaccactt cacaggcctc
    23401 gccacggttg attgccacca gtacacgttg ctgattcagc acgcggacaa ataccactac
    23461 gttatcttcc gcatacagca cctgacagcc gccacgacgc agcgcctgac ttttcttacg
    23521 cagcacaatc attcgccggt acagcgcgaa taacgccgta tcctgctttt ccacctgcca
    23581 ggggaacggt ttacggcaaa acggatcgtt tttaccatcc agtcccactt catcaccgta
    23641 ataaatgcac ggtacaccag gccaggtgaa cagccagacc accgccagcg gcaggcgcgc
    23701 aatatcccga ccgagcagcg ttttaaatcg cgcagtatcg tggctgtcga gctgattaaa
    23761 catacgtaat tgttgttgat gagaaagccc tgcgcggtaa ttatccatcc aggccataca
    23821 ggtttgggca tcaatttgct gcggatcgta agagatatcg gtattggcaa gaaatcccca
    23881 caacgggaat gtgaagccac gatagttcat ggcggcatct tccacatcgg cctgtaacca
    23941 ttgccgtgca tcgccaaaat gttcgccgac aatatacgct tccggctggg tttctttcgc
    24001 cgcttcggtg atcccagcaa cgtgctgcat attattgcgc gccccacccg cctcccccag
    24061 catatgcacc acatccagcc gccagccgtc catactccac ggcgctgtca gccaatggcg
    24121 gacaatactg tcttccccgc gataaatttc attcaccaga ctttccgact gataatccag
    24181 cttcggcaag ctggcatagc caagccagtc gagcgccgtg ccatcatcac taaacgagta
    24241 ccagtcgcgc cagggcgatt cagggttgta acaagctccc cccgtgccac gattgtgcct
    24301 gtcaaaccag gcatgggaat cgccactgtg gttaaacacg ccgtccagca ccagccgcat
    24361 tcccagctgc tgcgtattgt gtcgcaaacg cagcaacgcc ccatcaccgc caaactgcgg
    24421 atcgacatgg cgataatcct cggtatcgta ttgatgtacg ctgggagctt taaacaccgg
    24481 attgagatac agcgctgtca cgccaagctt tttcagatac ggcagttttt cgcttatccc
    24541 gtccagatcg ccgccataga acgttgatcc gcccgcctgc gccgtgaccg gttcatccca
    24601 gtcacgcaag atgatctctt gtccggctgc atgatggtaa tagacatgat cctgttcagc
    24661 ttcacgagga agactacgcg caaaacgatc agggaagatc tgataaaaaa tctgatccgc
    24721 agcccattgt gggccgatat ccggtacatc gacggcaaac tgctccagtc gtgccggcgg
    24781 cattcggctg aagccctgcg gtgacgcctg gctgcggctg actgcgctgc ttatgcattg
    24841 gtactgacat ttcttcgtta tcgtgttctg tacgcagcat aatgcgctgc ggtgggtctt
    24901 cgcccgtcag ccacagtgta atgagcagtt gatctttgct ttgtttaaca aatgggggca
    24961 ccggcaggtg ccatgcattt aacatcataa ttcccctgtg atgaaaatct gatcaccttg
    25021 ccatagccta atgcaggatc actcatcatc tgaaggatat ttgggggagg aggacggggg
    25081 cggataactt tcggaatagc cggatcagga tttcctcctg aaccggctta cagaacgcaa
    25141 ggattactgg ttttcagcca gctgacgatc gtggcgacgc ttaaacatcc agccaatcaa
    25201 cagcacaaca atccacgcga aaccgacata cagcgagata cgcgtatccg ggtgataacc
    25261 aatcaacccg ataataaaga gcaggaaaat cagcccgccg atggtcgttg ctaccccacc
    25321 cggcacttta aatttcagcg ccttaacttc ttctggcggc aaacggcgac ggaaggcgat
    25381 ttgcgacagc aggatcataa tccacaccca taccgtggcg aaggttgcca gcgaagcgat
    25441 caccaggaag acgttttccg gcatgatgta gttcagatac accgcaaaca gcagcgcggt
    25501 agtcatcacc agcaccgtaa cccacggaat accgcgacgc gacgttttgc tgaaaatttt
    25561 cggtgcgctg ccctgctctg ccataccgtg gagcatacgg cctacgccaa atacgtcact
    25621 gttaattgcc gacagcgaag cggtcagcac aacaaagtta agaatgctgg cggcaaaggt
    25681 aatgcccata tgctggaacg tcagcacgaa cgggctaccg gcagtgccaa cctgattcca
    25741 cgggtagata gacataatga cgaacagcgt accgacgtag aacaccagaa tacgcatcgg
    25801 cacggagtta atcgcacgcg gaatcgattt ctcaggatct ttcgcttcac cggcggtaat
    25861 cccgataatt tcgatcccac cgtaagcaaa catcaccatt tgcaacgaca ttaccatgcc
    25921 aagccagccg ttactgaaga agccgccgtt gctccacagg ttatgaatac cggtcggttg
    25981 cccgccgttg ccaatccccc agatgatgat gccgaaaccg gcgacaatca tgatgatgat
    26041 ggtggcgact ttaaagaacg agaaccagaa ttccagctca ccgaatacct tcacgctcat
    26101 caggtttacg gcgcaaatga tcagcaccac gctcagtacc caaatccagt gcggcaccgt
    26161 cgggaaccag acacccatat agataccaaa agcggtcaca tcggcgatgg cgacaattag
    26221 gatttcaaag cagtaggtcc agccggtaat gtaacctgcc agcgggccga ggttttcctg
    26281 cgcataacgc gagaaagagc tggcggccgg gttatgtacc gacatttccc ccagcgcacg
    26341 cataatgata tacgccgcga taccaccgat aatataggcc aacaacacgc tcggaccggc
    26401 cattttgatg gcgtctgccg aaccgtaaaa cagcccggtg ccaattgctg aacccagtgc
    26461 cataaagcgt atgtggcggg tacttagccc acgctttagc ttgttcttac tttccatcaa
    26521 ttcctaaccc atcaacacaa taaaaaacca cggggcatcg cagccccgtg gttaaaacaa
    26581 atgttcagtg atttagtgag cgctggaggt cacctgacga cctgccgcgc gatcccagat
    26641 aatggccaga accaccatca ccactgttgg cattaaccac gccaggcctt gttcggccag
    26701 cggtaaacgc tgcgcccagg acggtaagat atcgctgaat gcagatgctt tgatcccgtc
    26761 gagaatacca aaaagcaggc tgataaacat cggcggggca atcacgcggg acgaattatg
    26821 ccaccatgag cgtgtaaaac ttaatacaac cagtgcgata cacggcggat aaatagcggt
    26881 cagcaccggt acggagatct ggatcagctg gcttaagccg aggttagaca ccaccatcga
    26941 gaagccgccg aggataaaca ccagcgtacg ataagagagc ggtacgtact gggcaaagaa
    27001 ttctgcacaa gcacaggtca ggccaactgc cgttaccagg caggcgatga agattaacgc
    27061 cgccagcagg aagctaccgc cgccgccaaa tgtgtgctga acgtaagcat gcagaatagc
    27121 agcgccgttt gcagactgat cgaccagcga cgcgctgtct gaacccagac ggaacagcgc
    27181 caggtacagc agagtcagac caacacccgc catcaggcca gcccagacgg tataacgggt
    27241 cagcagacgc gcttcggtaa cgccacgaga acgcgccgcg ttaacaataa cgataccaaa
    27301 caccattgcg cccagcgtat ccatggtcag atagccgtta acgaagccgt tagaaaacgc
    27361 agcgttttga taagcctcag tcgccgtgct gatagaaccc gccggccaga caatagcggc
    27421 aacagacagg atgaccagcg cgataatttt cagcggcgca aggaagttgc ccacggtatc
    27481 cagcagcttg cccggataga gcgaaaccag aataacgata gcgaaataga ccaggctgta
    27541 gataaacagc ggcagcgcgg aatcacccgt cagcggcgca atccccactt caaaggaaac
    27601 ggtagctgta cgcggcgtag cgaaaagcgg ccccaccgcc aagtaacaaa ccgttgccag
    27661 cagcacgcca gcgactttac cgattggcgt gctgaggctg tcaacaccgc cgccaacttt
    27721 tgccagcgcc actaccgtta ataccggcag gccaacggca gtaatgagga agccgaatgc
    27781 cgcagtccag acgtgttcgc ctgcctgcaa gccgaccatt ggagggaaaa taatgttacc
    27841 tgcgccgacg aacaacgcaa atgtcataaa gcccagagcg atgatatcgc gcgatcttaa
    27901 ttgatgggtc ataaatcata ctgcctgtgg atgtggtgtt gtaacgttaa atttttgtgc
    27961 caatccctgg ggatgataca gcggtatatt taaacaattt tagcgggaaa tactcccaac
    28021 ctggcgcggc gaagaatagt ttttcgattt accgcgttaa tgcagtcagt atgacagcac
    28081 ctgaagcgca atttaaacgc ttataacgtt ttaaagcaag gagggatcta aaaaccagag
    28141 gtatatagcg ataagaaatc accaaccagt ataaaacact atttccgtga aaaagacatg
    28201 gctaatcatg cgaacaaaaa atcaccaacc gttgtaaaat ctaacgcccg tattttgact
    28261 gcaaagaaaa taagccagca tcgactggct tatggcaata aaagatgaca aaggcggatt
    28321 aatcgctgtt tttggcaatt aaacgttccg ggataacaaa actgaaacgt gttccttttc
    28381 ctactgtact ctcaatattc aggcgacttt cgtgatgatt cacagcatgt ttcacgatcg
    28441 ctaaccctaa tccgctaccg ccggtttgcc gggaacgcgc tttatcaacg cgataaaaac
    28501 gctcggtcag gcgcggaata tgctccggtg caatgcccgg tccgttatct tcaacgctaa
    28561 attcggcacc gtgcggcact cgctgccagc gtacggtgat atgcgtgcct tctggagtat
    28621 gattcacggc gttatagacc aggttcgaaa tcgcactgcg tagctgatct tcgttgccag
    28681 acactttgag gccgttatct atctcaaagg taaatgtctg ttttttctga ctcagagtct
    28741 gagcctcgcg ctcaacaacg cgcagcatca tcggcacatc aaccttttca ttgagcaatt
    28801 gcgtcggcgc ggcttcaatt ttcgacagcg ttagcaactg cttcaccagc ccttccatcc
    28861 gctgggtctg ctcgcgcatg gtgtgcaacg ctttttcgcg taccgcgcct tccagcggct
    28921 gctcatccat catctccagg taaccctgta acacggtcaa tggcgtacgt aactcatggc
    28981 tcacgttggc aaaaaagtta cgccgcgccc cttccagttg atgcatttgc gtgacatcac
    29041 gcgccaccat cagcaactgt ttgtgggtat aaggcatgac gcgaatttcc agatgccgcc
    29101 cggtgttgag caccagattg agcgggcgag aaaaatcacg cgttttcaga tattgcgtaa
    29161 actccgggta acgcagtagg ttaaggatgt tctgcccgtt atcttccggc cagcgcaaac
    29221 caagaatttg ttgcgccaga ccgttacacc agaaaatacc gccctcttcc gtggtcagca
    29281 ccaccgcgtc gggcagcgac tccgcgccgc tacgaaagcg tttaatcaga ttgcccagtt
    29341 cacggcggcg ttttttattt cgcagctgca tctggtgtaa gccgtatagt agcggttccc
    29401 agctaccacg ccccggtggc ggggtcatac tgcgatccac ccacaaccac catgaaaggc
    29461 gcaataaatt ccagaaatgc cagataagca gtcctgttac cgatgccagc aaaaaccagg
    29521 gcaggtaacc aaaaaatgca cccaggatga aagccgggag gcaacaaagt agcagctcca
    29581 gcaccagcct tttccacgac agccgttcca gcacgtaaga tactccagtt aagaaatcat
    29641 aagccctgct ctgcgtccga tgagcaaggc gttaaaagcg ggttgaaaaa cgatatcctg
    29701 taccgcgcac ggtctgcacc atgcggtcat gcccgccggg ctccagtgct ttacgcaggc
    29761 gacgaatgtg gacatcgacc gtgcggtctt ccacatacac gttagttccc cagacgtggt
    29821 ttaacagctg ctcgcggctg tacacgcgct caggatgcgt cataaaaaag tgcagcagtt
    29881 taaattctgt cggccccatc tccagcggct cttcgcccgc catcactcgg tgagatgtcg
    29941 ggtcgagact taatccctgc atctcaatca cctcttccac cgccattggc gaaatacggc
    30001 gcattaccgc tttgattcgc gccaccagct ccttcggcga aaacggcttg gtgatatagt
    30061 catccgcgcc ggtttcaagg ccgcgcacgc gatcttcttc ttcccctctg gcggtcaaca
    30121 tcaccactgg aatatcccgg gtcatcgact cgcgcttgag gtgtttgatg aactggatac
    30181 cggagccgcc aggtaacatc cagtcaagga gaattaaatc cggccagggt tcattcagtt
    30241 gattcacagc actgtcataa tcttccgctt cgaccggctg aaagccattt tgttcgagca
    30301 cgaagcagac catttcgcga attggagctt catcttctac gaccagaata cgtctcgcca
    30361 tgatttgccc tgttgtaata aataggttgc gatcattaat gcgacgtcat tatgcgtcag
    30421 atttatgaca gatttatgaa aagctcgtcg cacatatctt caggttattg atttccgtgg
    30481 cgcagaaaaa agcaaatggc acatctgttt gggtataatc gcgaccatgc tttttcgcca
    30541 gggaaccgtt atgcgcatcc ttcacacctc agactggcat ctcggccaga acttctacag
    30601 taaaagccgc gaagctgaac atcaggcttt tcttgactgg ctgctggaga cagcacaaac
    30661 ccatcaggtg gatgcgatta ttgttgctgg tgatgttttc gataccggct cgccgcccag
    30721 ttacgcccgc acgttataca accgttttgt tgtcaattta cagcaaactg gctgtcatct
    30781 ggtggtactg gcaggaaacc atgactcggt cgccacgctg aatgaatcgc gcgatatcat
    30841 ggcgttcctc aatacaaccg tggtcgccag cgccggacat gcgccgcaaa tcttgcctcg
    30901 tcgcgacggg acgccaggcg cagtgctgtg ccccattccg tttttacgcc cgcgtgacat
    30961 tatttccagc caggcggggc ttaacggtat tgaaaaacag cagcatttac tggcagcgat
    31021 taccgattat taccaacaac actatgccga tgcctgcaaa ctgcgcggcg atcagcctct
    31081 gcccatcatc gccacgggac atttaacgac cgtgggtgcc agtaaaagtg acgccgtgcg
    31141 tgacatttat attggcacgc tggacgcgtt tccggcacaa aactttccac cagccgacta
    31201 catcgcgctc gggcatattc accgcgcaca gattattggc ggcatggaac atgttcgcta
    31261 ttgcggttcc cccattccac tgagttttga tgaatgcggt aagagtaaat atgtccatct
    31321 ggtgacattt tcaaacggca aattagagag cgtggaaaac ctgaacgtac cggtaactca
    31381 acccatggcg gtgctgaaag gcgatctggc gtcgattacc gcacagctgg aacagtggcg
    31441 cgatgtatcg caggagccac ctgtctggct ggatatcgaa atcactactg atgagtatct
    31501 gcatgatatt cagcgcaaaa tccaggcatt agccgaatca ttgcctgtcg aagtattgct
    31561 ggtacgtcgg agtcgtgaac agcgcgagcg tgtgttagcc agccaacagc gtgaaaccct
    31621 cagcgaactc agcgtcgaag aggtgttcaa tcgccgtctg gcactggaag aactggatga
    31681 atcgcagcag caacgtctgc agcatctttt caccacgacg ttgcataccc tcgccggaga
    31741 acacgaagca tgaaaattct cagcctgcgc ctgaaaaacc tgaactcatt aaaaggcgaa
    31801 tggaagattg atttcacccg cgagccgttc gccagcaacg ggctgtttgc cattaccggc
    31861 ccaaccggtg cggggaaaac caccctgctg gacgccattt gtctggcgct gtatcacgaa
    31921 actccgcgtc tctctaacgt ttcacaatcg caaaatgatc tcatgacccg cgataccgcc
    31981 gaatgtctgg cggaggtgga gtttgaagtg aaaggtgaag cgtaccgtgc attctggagc
    32041 cagaatcggg cgcgtaacca acccgacggt aatttgcagg tgccacgcgt agagctggcg
    32101 cgctgcgccg acggcaaaat tctcgccgac aaagtgaaag ataagctgga actgacagcg
    32161 acgttaaccg ggctggatta cgggcgcttc acccgttcga tgctgctttc gcaggggcaa
    32221 tttgctgcct tcctgaatgc caaacccaaa gaacgcgcgg aattgctcga ggagttaacc
    32281 ggcactgaaa tctacgggca aatctcggcg atggtttttg agcagcacaa atcggcccgc
    32341 acagagctgg agaagctgca agcgcaggtc agcggcgtcg cgttgctcac gccggaacaa
    32401 gtgcaatcgc tgacagcgag tttgcaggta cttactgacg aagaaaaaca gttacttacc
    32461 gcgcagcagc aagaacaaca atcactaaac tggttaacgc gtctggacga attgcagcaa
    32521 gaagccagcc gccgtcagca ggccttgcaa caggcgttag ccgaagaaga aaaagcgcaa
    32581 cctcaactgg cggcgctcag tctggcacaa ccggcacgaa atcttcgtcc gcactgggaa
    32641 cgcatcgcag aacacagcgc ggcgctggcg catactcgcc agcagattga agaagtaaat
    32701 actcgcttac agagcacaat ggcgcttcgc gcgagcattc gccaccacgc ggcgaagcag
    32761 tcagcagaat tacagcagca gcaacaaagc ctgaatacct ggttacagga acacgaccgc
    32821 ttccgtcagt ggaacaacga actggcgggt tggcgtgcgc agttctccca acaaaccagc
    32881 gatcgcgagc atctgcggca atggcagcaa cagttaaccc atgctgagca aaaacttaat
    32941 gcgcttgcgg cgatcacgtt gacgttaacc gccgatgaag ttgctaccgc cctggcgcaa
    33001 catgctgagc aacgcccact gcgtcagcgc ctggtcgcgc tgcatgggca gattgttccc
    33061 caacaaaaac gtctggcgca gttaatggtc actatccaga atggatctct ggaacagacg
    33121 caacgtaatg tcgcacttaa cgaaatgcgc cagcgttata aagaaaagac gcagcaactt
    33181 gccgatgtga aaaccatttg cgagcaggaa gcgcgcatca aaacgctgga agcccagcgt
    33241 gcacagttac aggcgggtca gccttgtcca ctttgtggtt ccaccagcca cccggcggtc
    33301 aaggcgtatc aggcgctgga gcctggtgtt aatcagtctc gattactggc gctggaaaac
    33361 gaagttaaaa agctcggcga agaaggtgcg gcgctgcgcg ggcaactgga tgcattaacg
    33421 aagcagcttc agcgcgatga aaacgaagcg caaagcctcc gacaagatga gcaagcactt
    33481 actcaacaat ggcaagccgt cacggccagc ctcaatatca ccttgcagcc acaggacgat
    33541 attcaaccgt ggctggatgc acaagatgag cacaaacgcc agctgcggtt actcagccaa
    33601 cgacatgaat tacaagggca gattgccgcg cataatcagc aaattatcca gtatcaacag
    33661 caaattgaac aacgccagca gcagctctta acggcattgg cgggttatgc actgacattg
    33721 ccacaggaag atgaagaaga gagctggttg gcgacacgtc agcaagaagc gcagagctgg
    33781 cagcaacgcc agaacgaatt aaccacgctg caaaaccgta ttcagcagct gacgccgatt
    33841 ctggaaacgt tgccgcaaag tgatgatctc ccgcacagcg aagaaactgt ggcgctggat
    33901 aactggcggc aggttcatga acaatgtctc gcattacaca gccagcagca gacgttacag
    33961 caacaggatg ttctggcggc gcaaagtctg caaaaagccc aggcgcagtt tgacaccgcg
    34021 ctacaggcca gcgtctttga cgatcagcag gcgttccttg cggcgctaat ggatgaacaa
    34081 acactaacgc agctggaaca gctcaagcag aatctggaaa accagcgccg tcaggcgcaa
    34141 acgctggtca ctcagacagc agaaacgctg gcacagcatc aacaacaccg acctgacggg
    34201 ttggctctca ctgtgacggt ggagcagatt cagcaagagt tagcgcaaac tcaccaaaag
    34261 ttgcgtgaaa acaccacgag tcaaggcgag attcgccagc agctgaagca ggatgcagat
    34321 aaccgtcagc aacaacaaac cttaatgcag caaattgctc aaatgacgca gcaggttgag
    34381 gactggggat atctgaattc gctaataggt tccaaagagg gcgataaatt ccgcaagttt
    34441 gcccaggggc tgacgctgga taatttagtc catctcgcta atcagcaact tacccggctg
    34501 cacgggcgct atctgttaca gcgcaaagcc agcgaggcgc tggaagtcga ggttgttgat
    34561 acctggcagg cagatgcggt acgcgatacc cgtacccttt ccggcggcga aagtttcctc
    34621 gttagtctgg cgctggcgct ggcgctttcg gatctggtca gccataaaac acgtattgac
    34681 tcgctgttcc ttgatgaagg ttttggcacg ctggatagcg aaacgctgga taccgccctt
    34741 gatgcgctgg atgccctgaa cgccagtggc aaaaccatcg gtgtgattag ccacgtagaa
    34801 gcgatgaaag agcgtattcc ggtgcagatc aaagtgaaaa agatcaacgg cctgggctac
    34861 agcaaactgg aaagtacgtt tgcagtgaaa taactattca gcaggataat gaatacagag
    34921 gggcgaatta tctcttggcc ttgctggtcg ttatcctgca agctatcact ttattggcta
    34981 cggtgattgg tagccgttct ggtggttgtg atggtggtat gaaaaaagtc attttatctt
    35041 tggctctggg cacgtttggt ttggggatgg ccgaatttgg cattatgggc gtgctcacgg
    35101 agctggcgca taacgtagga atttcgattc ctgctgccgg gcatatgatc tcgtattatg
    35161 cactgggagt ggtggtcggt gcgccaatca tcgcactctt ttccagccgc tactcactca
    35221 aacatatctt gttgtttctg gtggcgttgt gcgtcattgg caacgccatg ttcacgctct
    35281 cttcgtctta cctgatgctc gccattggtc ggctggtatc cggctttccg catggcgcat
    35341 tttttggcgt cggcgcgatc gtgttatcaa aaattatcaa acccggaaaa gtcaccgccg
    35401 ccgtggcggg gatggtttcc gggatgacag tcgccaattt gctgggcatt ccgctgggaa
    35461 cgtatttaag tcaggaattt agctggcgtt acaccttttt attgatcgct gtttttaata
    35521 ttgcggtgat ggcatcggtc tatttttggg tgccggatat tcgcgacgag gcgaaaggaa
    35581 agctgcgcga acaatttcac tttttgcgca gcccggcccc gtggttaatt ttcgccgcca
    35641 ccatgtttgg caacgcaggt gtgtttgcct ggttcagcta cgtaaagcca tacatgatgt
    35701 ttatttccgg tttttcggaa acggcgatga cctttattat gatgttagtt gggctaggga
    35761 tggtgctggg aaatatgcta agtggcagga tttcaggacg ttattcacca ctgcgcattg
    35821 cagcagtgac tgactttata attgtactgg cactgctgat gttctttttc tgcggcggca
    35881 tgaaaacaac gtcgcttatt tttgctttta tttgttgcgc gggattattt gccctttcag
    35941 caccgctaca aatattgtta ctacaaaacg ccaaaggcgg agagttatta ggtgccgcag
    36001 gtgggcaaat agcgtttaac ctcggtagcg ccgtcggcgc atattgcggt ggtatgatgc
    36061 tgacgctggg gctggcatat aattacgtgg cgctgcctgc cgccctgctt tcgtttgctg
    36121 cgatgtcgtc gttgctgctg tatggtcgct ataagcgcca gcaagcggcg gatactccgg
    36181 tgcttgctgg cgaaaccact ggggtaggtt atagtctcgg tgttcattac ttattgccgg
    36241 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgca ggcctgataa
    36301 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    36361 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttacgcaccg
    36421 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcggaacc gtttgatata
    36481 aacggtctac attgctcatc cccccccccc caggacaatc acatccggat cgagaatatt
    36541 cacgacatgt gccagcgatt ttgccagccg cagctcgtag cgacgcaatg ccagttccgc
    36601 taccggatcg ctttcttcaa ccaggcggat aatttcactg cctttcagcg catgtccgct
    36661 caaacgacga taatccatcg cgaatcccgt gcccgaaata aaggtttcaa tacaaccttg
    36721 tttaccgcaa taacaaggga cttcctcgcg ataacgcagt tcgtcttcgt ccatccacgg
    36781 tagcggattg tgtccccact cacctgccgt gccattgccg ctgatatgcg cccgcccatt
    36841 gaatgccacg cccgcgccgc atcccgtgcc gataatcacg gcaaataccg tctgcgctcc
    36901 cgctgccgcg ccatctactg cttctgaaac cgccagacag ttagcgtcat ttgccagccg
    36961 cacttcccgc tgcaacctcg cgcttaagtc tttatcgaat ggctgaccgt tgagccaggt
    37021 tgaattggca ttcttcacca caccggtgta aggcgaaatt gagccaggaa tgcccatacc
    37081 taccgttccg cgctgccccg tcgcctgctc cgccatatca accaacgtgg cgatcgtttc
    37141 aatagtctgc cggtaatcat cacgcggcgt gggcagacga tggcggtaca actgctcccc
    37201 tgcatcgccc agtgcaatca cttcagtttt ggtgccgcct aaatcgatac ctatacgcac
    37261 ggtactctcc ttattttttt caatatcaat agcgtagaga cggacaaccg gattggcaat
    37321 gcaaggccgc cgacaattcg ttatcatgcc cgctaaattt aacaacaagg ccgtggaaat
    37381 tatcatgctg tggttcaaaa atttaatggt ttaccgtctt agccgcgaga tttcgctgcg
    37441 tgcagaagag atggaaaaac agctagcctc gatggcattt accccatgcg gcagccagga
    37501 catggcgaag atgggctggg ttcctccgat gggatcgcac agcgatgcgt taacgcacgt
    37561 tgccaatggt caaattgtta tctgcgcgcg caaagaagaa aaaatcctcc cgtctccggt
    37621 gattaagcag gcgctggaag cgaaaatcgc caaactggaa gcggaacagg cgcgtaagct
    37681 gaagaaaacc gaaaaagatt cgctgaaaga cgaagtgctg cactctctgt tgccgcgtgc
    37741 tttcagccgt tttagccaga caatgatgtg gatcgacacg gttaacggtt tgattatggt
    37801 ggactgcgcc agtgccaaaa aagcggaaga tacgctggca ctgctgcgta aaagcctggg
    37861 gtcgttacct gtggtaccat tgagcatgga aaacccgatt gaactgacgc tgaccgaatg
    37921 ggttcgctcc ggtagcgcgg cacagggctt ccagctgctt gatgaagccg agctgaaatc
    37981 gttgctggaa gatggcggcg tgatccgcgc gaagaaacaa gatctgacca gcgaagagat
    38041 caccaatcac atcgaagccg gaaaagtggt gactaaactg gcgctcgact ggcagcagcg
    38101 cattcagttt gtgatgtgcg acgatggttc gctcaagcgt ctgaagtttt gcgacgagct
    38161 gcgcgatcaa aacgaagata tcgaccgtga agatttcgcc cagcgttttg atgccgattt
    38221 catcctgatg actggtgaac tggcagcgtt aattcaaaac ctgattgaag gattaggtgg
    38281 cgaagcacaa cgttaattgc tgattttcct ttaatgccgg atgcgacgcc tgccgcgtct
    38341 tatccggcgt acgaagccac accagggata taattattcg ctacggcgga caataatttt
    38401 tagcgcagca atattatgcg ttttatgctg taacttgctc catggacgtt gtgtcattgt
    38461 ttttcctcaa gccagtgacg cgcttcactg cgaattttcc gcattgcccc ggttttcact
    38521 aaatcccgta acagcgaaag atcgggttgt tcgacaaata agagtaatga tggatcctct
    38581 tttaaccacg tcgttttcac atcggcagcg agctgtggat tattcatagc tagcgaccga
    38641 tgccgagact cggtcaatgt cgttaacaac gacgccgggg tatgtgcgtt gcgtaatgcc
    38701 gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc atcatctgat
    38761 ttatctttta tcgtagccag ttccgcaaca atatctggag atcttcactg atatttatat
    38821 caaccttgat gaattatgaa tatatcggaa attgaaaaaa tcgcagcaaa ggaaatatta
    38881 ttatgggtta aagagaggat atttcagaaa aatgccctcc cgttcaaggg gaaggcgagg
    38941 aattacagat agcggcacag ataagaggtg ggttcggcaa cttgcagatg aaactcactg
    39001 tgaccgggaa cattaaacac cgaaccggct tcatacacct gccagtcggt cgcatcaggc
    39061 agtaacacat tcagcgcgcc actgattacc gtcatctctt ccggctcagc ggtgctgaag
    39121 gtgtattcgc cttcaaccat aacacccacg ctggcgcgac cagtgctgct gctggaaaag
    39181 ccgattgatt tcactttgcc ggaaaagtac tcattacttt gaagcataaa ctggccctgt
    39241 ctggtagttg aacatctact tcactatagg ggccagaggc gctggctgtc acgcaaaatt
    39301 acaccagtaa ttccgcagcc agccgtgcaa tcaatacgtt agacagcaag acaggaacat
    39361 cgagctgttt ttgcagtaaa tcgcgatgac gttggtgaaa tcctaaacaa tccagcatga
    39421 tgacatcagc cccttttgcc agtaattctt tccctgcatc aatgattttt tgttctgaat
    39481 catgaatggg gttacccaat gaaaataccg gcgatttctg caaaatttgc catttttgcg
    39541 cctgaacggg cagcatctcc tcaaccggaa cgataacccc cacctgatga tcttcaacaa
    39601 tagaggaaac cagtggaggc aatattcgcg acggctcaag aaagatcgta ttacgcgcag
    39661 tcatactact aatgtttgct gtactcatta ataaaatgac gtcataaccc tgattatcga
    39721 gcacttcaac cacaccttgc aggtctcgct ccactttacg acgcgaaaca tgggccagct
    39781 ggttgtcatt taataatgtg agaatggtgt cttcgcctgc ttctggcgcg tactccgcca
    39841 tcacttcttc acgacttaac ttccccagca ggctatgatg ggaaatatta tcttcgtcaa
    39901 tgtattccgt caggagcggc aaaacctcct gcataggtac aatgccgatg gtcaggatcg
    39961 ccaacgacgc actcattgtt catccacctt acttttcttt cgtcgttaat taccgggcaa
    40021 gtgtgaagca ccactgctga cacttacttg tgctacaaat gacaaaaagc gtagcagcag
    40081 acgtgcacgg cataatgtaa agattccaaa tgattccagt aatggattgt tattgtttaa
    40141 tattctaatt attagaaaaa catgaattat gaaaaatgtg acgcagatcc cttgtagtgg
    40201 cactcagaat cccttccgag cagtctgatg cggtcactct ttgtcagggt cctcatagcg
    40261 ttttttcgca tccatcgctt cctgagcggt cggatgttca ctgatcaacg agtctggttt
    40321 aggatgatcg gctctgagtt ggtaccaggt taccgtctgt cctggctttc ctttttcaat
    40381 cgtcactata tatgcttcac gaggataagg cggtttcgtt ggcatcgttc ttccttattt
    40441 cacggaatga acgttaagta taggcgctct aaaatcaaca attgatcgtc tgtgccaggg
    40501 cgctacgaat ttcagaaatc acctggctgg gttcgtttgt tgcgtcgatg ataatatgcg
    40561 caacttcgcg atatagcgca tcgcgttctt ccagcacttc ctgaacttct tcgctcagcg
    40621 gttttcccgt taaggttggc cgtaaatctt cttccggtgc agcttgcagt cggttaacca
    40681 ggactgatac tggcgcacac aaataaacca cgatcccgtt attttgcatg aagtgacgat
    40741 taaattccgt cagaataatg ccgccgcctg tagcgataac ggtggatggc gcagttaccg
    40801 cttccagcgc cgccgtttct ctggcgcgaa atcccgccca ctcttccctt tcgacgatct
    40861 ccgcgaccgt catattgagc tgtgattgca accactgatc ggtatcgaca aaccgacggt
    40921 taagcgaatc ggcaagggcc attccgaccg ttgttttacc acagccccga ggcccgatca
    40981 gaaaaagagg ttgtgtcatc gtgggttttc cccaataggt cgcaatgcgg cgaaagccgg
    41041 tgtcatgaga atagcgatca ttccatcaaa ctagtacaat ttcgattgta aagaaaaaat
    41101 tccacttaaa gtgaaaatct caatacatcc cttactatac caataaatat tcaagaatga
    41161 agtgtaaata ataaattaca tttagccacg actacgttgc acttccagcc accacttctc
    41221 cagctccgcg gcaaaggcct ggcggtcacg ttgtgaaagg ctatctggtc cgccggtctg
    41281 gatcccactg gcacgtaagg tatccataaa atcgcgcatc gtcaggcgct cacgaatggt
    41341 cgctggcgtg taacgttcgc cgcgcggatt aagcgccgct gcgcctttct cgatggcttc
    41401 agcagccaaa ggtatatctg cggtgatcac caaatcgccc gcttcacact gccggacaat
    41461 ttcgttatcg gcaacgtcga aacctgccgc gacgcgcagc gtacgaataa atcgcgatgg
    41521 cggcacgcgt aaactctggt ttgctaccag taccagcggc atctgcatac gttccgccgc
    41581 gcgatacaaa atctctttaa ttacattggg acacgcgtca gcatccaccc aaattgtcat
    41641 aaagtcatcc tttgttgggt aatcctctat tgtgtcgcgc ttttgccttc cggcatagtt
    41701 ctgtttatgc ttctgccagc gattatcaaa acaatgaatt tcacggcagg agtgaggcaa
    41761 tggaaaagaa aatcggtttt attggctgcg gcaatatggg aaaagccatt ctcggcggtc
    41821 tgattgccag cggtcaggtg cttccagggc aaatctgggt atacaccccc tccccgaata
    41881 aagtcgccgc cctgcatgac cagttcggca tcaacgccgc agaatcggcg caagaagtgg
    41941 cgcaaatcgc cgacatcatt tttgctgccg ttaaacctgg catcatgatt aaagtgctta
    42001 gcgaaatcac ctccagcctg aataaagact ctctggtcgt ttctattgct gcaggtgtca
    42061 cgctcgacca gcttgcccgc gcgctgggcc atgaccggaa aattatccgc gccatgccga
    42121 acactcccgc gctggttaat gccgggatga cctccgtaac gccaaacgcg ctggtaaccc
    42181 cagaagatac cgctgatgtg ctgaatattt tccgctgctt tggcgaagcg gaagtaattg
    42241 ctgagccgat gatccacccg gtagtcggtg tgagcggttc ttcgccagcc tacgtattta
    42301 tgtttatcga agcgatggcc gacgccgccg tgctgggcgg gatgccacgc gcccaggcgt
    42361 ataaatttgc cgctcaggcg gtaatgggtt ccgcaaaaat ggtgctggaa acgggagaac
    42421 atccgggggc actgaaagat atggtctgct caccgggagg caccaccatt gaagcggtac
    42481 gcgtactgga agagaaaggc ttccgtgccg cagtgatcga agcgatgacg aagtgtatgg
    42541 aaaaatcaga aaaactcagc aaatcctgat gactttcgcc tgacgtcagg ccgccacttc
    42601 ggtgcggtta cgtccggctt tctttgcttt gtaaagcgcc aaatctgccg atttcaacca
    42661 ctcacgatag tgactcattt gtgggttcag cggcgcaacc cccacactaa tccgtaaagt
    42721 tacctgtggc gtattcggca agcgtaacgt gtttagccct tcacgcaccc gtaacatggc
    42781 ggtaatggcg ctctcagctg gcgtaccgga catgattact gcaaactcat cgccgccaaa
    42841 ccgaccaatc acatcgctac cgcgcagggt aatttgtaac tgtcgggtaa gcgccacaat
    42901 cgcttcatcg cccacatcat gaccccaggt atcgttgatg ctcttgaaat ggtcgatatc
    42961 gataatcagt aacgttgcat cgcgattatg ccgccgacag ttatcaaatt cattgcgtaa
    43021 catagtttcc caatgacgtc ggttatacac gcccgtcatg ccgtcgcggg tactcatgac
    43081 ctgcaacctg cgtttatgtt ccgccagttt ggttgccgtc tggtagctga cccagccaaa
    43141 cagcagagga taaacgacaa taatgggaag ggagagccac cattccagcg gcgcactatt
    43201 gaacgacacg gtaatgcccg taagctcgag ggtgacaagg caggaaacca ccatcaacac
    43261 cagacccgcg acaaacagac gggggccgcc tgcccccatc aaattcagac acataatcat
    43321 caacatagcg gtggaaggca gcacgtttac gcccattacg cctacccaca ttcccgctaa
    43381 tactgcatcg gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg ccctgctcgc
    43441 tatctgccag gctaaatgcg gccagacgaa cgcccagccg accaacacta gccaccacca
    43501 gcccggcggc gggtgcgaaa ccagcgttga agcaatcggt aagaacatgc cagccaggcc
    43561 aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct gatgttcgtt
    43621 ttgaggagtt aaaggaggtt cctccccatg cgccgccgct tttttgaaaa agttttcatc
    43681 attcattatt tttgggaaca ttcagaaaca attttcccaa attatagaga cggggctaaa
    43741 ggcagaagta tgaaatttcg ggtgagccac tttcgtagct cacccgttag cactatcagg
    43801 tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc acctttcaga
    43861 gattgttgcg ttgcttgatt attacattcg cgcatctttt gctgctgtgg cgtcaaactt
    43921 ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt cttacgagca
    43981 tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg ctgttgttgt
    44041 ggggttaacg ttctctcggc agcgccgact gtggttaaaa aaaccagacc gaaaagcaag
    44101 gtaaccagta atgtaatttt catagcacca tccctcttca tgttttaacc atgagcgtat
    44161 gcgcccgaga tttgccatta agtctggttg ctaacagcaa aaaaaccacc cggcagcgaa
    44221 aattcactgc cgggcgcggt tttatttcag ccccagggcg gctttcatgg tgtagaagag
    44281 atcggtctgg tcggtcagtc caacgacatt ggcggcatgc gggccatacg ccgcaatacg
    44341 caactgactg ccggtatgtt cttgtgaatc ctcttcggag ttcccgtagc tcatcaccat
    44401 cactgcgcca tctttggtat ttagcgcctg ggtgaggccc ggagctttgg tatccggcgc
    44461 gacaatctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt tgccatcctt
    44521 tttagcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct cgccaatttg
    44581 cccacaagga ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa cttgcaggaa
    44641 aaatcctttc tcatttttac tcaacaattc aatggctttg tcggtcatct gcgccagggt
    44701 tggtacactg tcattacgtt gcggattagg cgtacaggtg actgcgggct tatcgatatt
    44761 gccgtggtac gttgctttcg gtcctagcca gcgcactggc atattgccgt cagcaaacag
    44821 tccaagcaga ggtttttgct gattcgcttc cgtcaccgca tttagcgagg cggcatcgct
    44881 gaccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct gccattcacc
    44941 ggcggttgcc gtttcagcaa aggtttttgc gccaccgcca agcgtaacgt cggcacgagc
    45001 attaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt tacccggaca
    45061 tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg ccaccagcgc
    45121 agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca gacctgcggc
    45181 ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc ccagcgcgcc
    45241 gttataggtt ttgacaccgg ttgaccaggc ggttgctgat gcagccgaat cggtgacgta
    45301 gtccggtttg ccggtttttt tattcagcgc atagtgagtg tattgcccgg taagcggtaa
    45361 ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg cggctgtaat
    45421 ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag gtttatcgct
    45481 aagagaatca cgcagagcgg cagtctgatc acccgttaaa cggcgagcac cgccgggtgc
    45541 agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg tccgggcttt
    45601 tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt gtttcacttt
    45661 attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa gtctcggccg
    45721 tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacgagga tgcttcaata
    45781 acttctttac ataattgcgc agcagctccg tatcgtcgtc aggaatgctg gcatcaggct
    45841 ttacctcgta aagagccccc tccacctgtt caatcaagcg ctgttggtca ttttgtgcca
    45901 tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgcg cacagttctt
    45961 tcgactcttc ttctttttgg gcaagcttta ataacaactc agcaatgaga tttttcatgt
    46021 ctgtatttcc ttatccaacg tatggagaag ttaacattat gtttcacaac ttcatagtgt
    46081 ctggaagtat tatttttatg acaaaggaaa tactttgcgc aaatgttctt tgtttgatta
    46141 ccagcgaaac gtttctcata tttaaataat taaccacaca aagaacaggc ctttaattat
    46201 caacctttat tataaacaaa agttatgaaa tatattctct gcgtttactt acaactaaga
    46261 gaaaaaactc accgcatggc catttaagcc aggaataaat tagcttcccg ttcatcattt
    46321 ttgtgatctc gcgcacatct ttgtaataac gcactttctt caccattacg ttgcacgctt
    46381 tgtcagcaga agattgcaac attctgtcag aaggccgcga atcccccgtt ttgtcgtggt
    46441 aatgtatgca ctttgtgtta gacgggatag gtttttaaga tggaaaaact gcgggtagga
    46501 atcgtttttg gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc aaaaaacatt
    46561 gtcgatgcca ttgataaaag tcgcttcgac gttgtgctgc tgggcattga taaacaaggg
    46621 caatggcacg tcagcgatgc cagcaattat ctgctaaatg cagacgatcc tgcccatatt
    46681 gcgttgcgcc cttcggcgac tagccttgcg caggtgccaa gtaaacatga gcatcagctt
    46741 atcgacgcgc aaaacggtca gccgttaccg acggtggatg tcattttccc gattgtccac
    46801 ggtacgctgg gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa tttaccgttt
    46861 gtaggttctg atgttctggc ttcagcagcc tgtatggata aagatgttac caaacgtctg
    46921 ctgcgcgatg ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc caatcgtcac
    46981 aacattagtt ttgccgaagt ggagtctaaa ctgaggttac cactgtttgt aaaaccggct
    47041 aatcagggct cttctgttgg tgtcagcaaa gtaaccagtg aagaacagta cgcaattgcc
    47101 gtcgatctgg cgttcgaatt cgatcataaa gtgatcgttg agcaagggat caaaggtcgt
    47161 gagatcgaat gcgcagttct gggcaacgac aacccgcaag ccagcacctg tggcgagatc
    47221 gtactcacca gcgacttcta tgcctacgac accaagtaca ttgacgaaga tggcgcgaaa
    47281 gtggtagttc cggcagccat tgcgccagaa atcaacgata agatccgggc gattgccgtt
    47341 caggcttatc aaacgttggg atgcgcaggc atggcgcgtg tagacgtgtt tttaacccca
    47401 gagaacgaag tggtgatcaa cgagatcaac acactgcctg gcttcaccaa catcagtatg
    47461 tatccgaagc tgtggcaagc cagcggtctg ggttacaccg atctgatcac acgtttgatt
    47521 gagctggcgc tggagcgtca cgctgcggat aacgcattga aaaccacaat gtaatatttt
    47581 aacgcccgat aatattctta ttgggcgttt tcagtttctt catcgcgacg acgaataatc
    47641 aaccccgcca gccagaaact aatcacccac gtcaccagac cgacggcata ggtctgccaa
    47701 ccttttgctt caaatcccag taaccccacc gcgccattaa gaatgaatat aaggccaatg
    47761 gcgaacgcat agtagtgcca gtcacggcgg atttttacag gcagattcat ggcagctccg
    47821 ttaacgaaaa acaacatcct tgcatattct gtaacaaccg tctgtggtgt agacaacatt
    47881 ttcgtaattg ttaataaatt taacttaagg ttgccatttt gtgactcaca acgaagattc
    47941 acgcttcggg aatattccca acatcaattt accatgattc tgatattgac aatctaaatg
    48001 acctgtcaga cttgccataa gccgctggta tcacaccaga agagaagcag atgacttccg
    48061 gaggttttta tggctgattt caccctgtca aaatcgctgt ttagcggaaa atatcgcaat
    48121 gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg tgctgttttg cttttgggct
    48181 ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg tctatgagcg tttaatgcag
    48241 gtccaggaaa caggtcgccc acgggtggaa attggtttag gtgtcggcac cattttcggg
    48301 ctgatcccgt ttttagtagg ctgcctcatt tttgcagtgg tggcgctatg gctgcactgg
    48361 cgacatcgcc gccagtaaga tttacgttgc cagatacggc ctaaaacgcc ttatctggca
    48421 tgacattcct gtttgatcac tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg
    48481 caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct ttccagttga atgccaggca
    48541 acatttcgcg gggtagtgat ttacccgttt tcgcttcggc aagttgataa actttcttgt
    48601 acagcgccgt ctcctcaaaa gagaagctgt cacctttctc taactgacgg cgaatttcgc
    48661 tgtcgctcat ccccagtttt cccgccagtt tgcgcgttgc cagttcggtt ttcccgggtt
    48721 ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg ctggccttac
    48781 tgaccgcgtt ctgaaacgcg gcattacggc tggcgtacca gccagcgtta aaatcagcaa
    48841 agcggtatat cggtgcgcta tagctggcgg gataattcag taaatggtaa gtaccaaacc
    48901 acaacccgcc gcggcgactg aagacttcct gacgaactgt accgtccatt ttccacggat
    48961 accctttggt atgctgttcg gcaaaagcaa tgctgacctg catcggccca ccggtgcgca
    49021 ccggattaag cgaaccaaac agcgtctgcc ccattggtac catgctgatc agatcgtcaa
    49081 aaatcgcgct caattgcttt tctgtacgca ccgaatctaa ccgttcgcta taacttttgc
    49141 cgtttggcga tttgattttt agcgcggtat gaaccagaaa agcaggaatg tgcatccgtt
    49201 cagcacgacg gtcaatctct tgccaggcga tcttgcttaa gcccggaacg gccggatctg
    49261 cctgataatt tgactcctgc tgcgccaccg ccagcaccga gcagacgttt tccagcgtcg
    49321 gtgccagccc ctgactttca aaggtggtgg cgagatcttt cgcccaggca tcgcggtctt
    49381 tcacgctcgc aggcatcttc tgccgcacga cgctcgctac atctatcgct ttttcgccct
    49441 ttttcaacgg ttgcggagcc tgactactac accccgctaa aaccagaacc gccaacaatg
    49501 acagcgaaga tagatgattg acgcgcgaca ttacttctcc tttttagctc aaggtatggg
    49561 ttacttcctg aattttgtca ccatccagct catgttcaaa gctgcgcaga cgtttgtaga
    49621 tagacatcaa ctcaaccagt gtggtccatg agttaatcag gtactggaaa gcaccgcgaa
    49681 cctgaccaaa aacgttggta atctgcgtca tcaggccgag cgtaatcgta ccggcaacaa
    49741 ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag agaatgcggg
    49801 cgatgttgaa atacatatag tgaaaataga ggcgaaaata gtttttccgt acggcgctaa
    49861 acagctcgcg taccgtaggc ggcgtcgcgc gcgtggcatc gtcttcacca taaaccagct
    49921 ctttacggta ggcggcctct actcgctggt ttttaaactc cagccccggc agtttgatcc
    49981 ctaccactgc cagcaatccg gtccccatca gcgaccagac gatagcggca atcaccagac
    50041 catacggaat gtgcccgata atcggcagct ccggcacatg cgcggagagc gttaccagca
    50101 ccggcaggaa ggcgaccaac gtcatgatgg cgttgataaa actgacgccc atattctcca
    50161 gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg tgcggcacct tcgatatgac
    50221 gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag cggaacacat
    50281 agtgactgac aaagaagttg ttcagtacac tgatcaccac cgcgatcagc gcaatcccca
    50341 gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac tttatgcggc gaacttagcg
    50401 cggtttgaat cagatcatag aacggtgcat accaggcgtt gacggcgact ccgacttcca
    50461 ccaaaaacca ggtgacgaag atgatcagtg cagtaccgag tattgaccag tattgccaac
    50521 ggtgcgggct gtagataaac cagaaaaatg caaaaagtcc tacgcaaaca atgtagtaag
    50581 cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc gctaatcggg atctgcccgg
    50641 aagcgccggt gatacgcgcc acccagtctc ccccaccggc ttgccagaag ataacggcaa
    50701 tcaatgccca aacaaaggcc gagagaaaaa acgctcccgg ctttgggaaa aaagacttaa
    50761 acatatgcac tcctgctaac ttcttatcgt tttaacaact gctgtgtatt aaccgcatga
    50821 ccactacaaa gcgtcagcca aatgaaaccg cccattgtga cccatagcaa ggcgcttagt
    50881 tcgccagcct gaccataaga attgtttcga cggatttcac aaaccaaaga ttgtttacat
    50941 aagcggatta acacgcgcag gatcacaaat gcaggcattg gtgagcagga aaaagatcgt
    51001 gtttacgcgc cgttacgttt ttaacactat tattgcacta attcgccctt tgcaatccat
    51061 caatgagtag tataaatacg ctcagttacc ttcattcaat ctatggacac caccgttgaa
    51121 acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg tccatgcggc
    51181 gcagccgatc actgaaccgg agtttgcctc tgatattgtc gatcgttatg ccgatcatat
    51241 tttttacggc agcggtgcca cggggatggc gctggttgtt atcgacggta accagcgcgt
    51301 ctttcgcagt tatggcgaaa cgcgacctgg taataacgtt cgcccgcagc tggattcggt
    51361 cgtgcgtatt gcttccctca ccaagctaat gaccagtgaa atgctggtga aattgctcga
    51421 ccaggggacc gtgaagttaa acgatccgct aagtaaatat gctccgccag gcgcacgcgt
    51481 gccaacctac aatgggacgc ccattaccct agtgaatctg gcaacccata ccagcgccct
    51541 gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc caacgcgcga
    51601 gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc gcgccaggca gtcaggcggc
    51661 gtactctaac cttgcgttcg acctgctggc agatgcactg gcgaatgcgt ccggcaagcc
    51721 ttatacccag ttgtttgaag agcagattac ccgtccattg gggatgaaag acaccaccta
    51781 caccccctca ccggatcagt gccgccgttt aatggttgct gagcgcggtg ccagtccgtg
    51841 caataacacg ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg gcgatatgat
    51901 gcgctggatg cagcagtatt tatcatctga tttttatcag cgcagtaacc aggcagatcg
    51961 catgcagacg ttgatttatc agcgcgcgca gtttaccaaa gtgattggca tggatgtccc
    52021 cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg gccccgaaag agggtcgtcc
    52081 ggggattatt cagaagacag gcggtggtgg cggtttcatt acctatatgg cgatgatccc
    52141 gcagaagaat attggcgcgt ttgtggtggt cactcgttcg ccgttgacgc gctttaaaaa
    52201 tatgagcgat ggcatcaatg atctggtgac cgagctaagc gggaataaac cgttggttat
    52261 ccctgcatcc tgattaatac tctgaaggca aacggttgat agcgaccagt ttgcctttat
    52321 tcatttcgat atagccgcct ttacgcaaag cagcgagaac ttctgcgacg acagaacgcg
    52381 atatgcgtgt acgttgatgg atataattca tcacaccaat acgtgatcgc aattcttcat
    52441 tccagtcaat cattgataat aatgttgcgc ggatttgttc gtaggaatta tgcccaatga
    52501 gctgcacgtc gcgtaattcc agaattctat tttgccaggc taaccagtaa aaagcgtctc
    52561 gccagagttg attttgttca ataagcgtaa tggtttgttt ggctggtaga tgatatcccg
    52621 tacaatttcc ttctgatatt aatttgtatg gtatatcgtt tttcattaaa ccatcagcca
    52681 gccccataat ataagaagcc tgggtaatac cgataagtac gttttcttct ctacgcagag
    52741 agataacgcc ctctaaaata aagaaagtat cttcgctatt gacatcactg gaaaatataa
    52801 cttgcttttc attattaaac tcgaagcgcg taccgtatct ggacaaacat ttatcgagct
    52861 taccaaattc ctgaagaggt ttaactacag ataacatttg cgcgtccttt gcagtaatgc
    52921 ccgtcaaatc cttgacgggc attatttaga ttaaattacc agaatttctt cggagtgaag
    52981 aatattacca agtatattta acacccacgt tcgcggacca gtcttgatct acgtcaccac
    53041 caccgaggta gttagcatcg gtataggcgc tgaagttctt ggtgaagctg aactgagtac
    53101 ccagaccaac acgtaccgca gacccttcag taccgttatc gatggaatca ccgttcacat
    53161 cgttatcgtt gtttgagtcg tcgtagacgt aagccagttt gaagtacgga gtcagagcct
    53221 gatcttcgct gtaggtgaag gtataacctg catctacacc cagttcataa cgcatgctgt
    53281 cgtaagactg accgtcaact ttcatgtcgt tgctcagctg gtagtcatca ccagactgga
    53341 acagaccaga aacgctgccg taaggagtct cgtaaccagc atcacccagt ttgaagtcgt
    53401 aaccggcttt caaaccgaag ccccacgcgt cagagttggt gctaccgtca acgtaagtac
    53461 cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga gtagctcaag ctaccatcaa
    53521 caaagacgtt gttcgcgaag tgagcagaag agtagatgta ggcagtctgg ctgtcttgat
    53581 ccacctgacc agaacggtca ttcatgtcac ctttagcgaa gcctgcagcc gcaccgacga
    53641 tccacttagc gttgttaccg tcaattttgg tatcaacacc gaccatgatg ccgttaacat
    53701 cctgatcata gttgatggtg ccgttgtcgc cgttgaagtt accaccgaag tagcttaccc
    53761 atgcgccgcc gttatcagcc aggccatgac gagagttggt cagacgagta ccaacggtgt
    53821 cttgttccag gttccagata ttggtgttcg cagacgggat gctcagcgcc atgttagcgt
    53881 agtcggtcag ctccatctgt tgcagaacaa cggtgttacc gcgctgttca gcctgatagg
    53941 tgtatgcacc caggtcagct ttgttagcag cagagaaggt cgcgttggtg ttagcgtcgt
    54001 ttacccgaat aagttcttta tctttgtaat cagcgatagt gccagcgcca gttgcgttgt
    54061 cgatacgaac tttgtagtta ccggttgcag caacaacgtt atcaacagag ctgttatcca
    54121 gttcagtacc gttgtctacg tcgccgttac cattgatagt caggtgacca tcagagttca
    54181 tagcaacaat accgtaaccg tagttagact tggaagtatc ccacgtacgg tcgttcacca
    54241 gatcagcgtt cagaacgtaa tcgctgctgt tgatgtcgaa cacaccagaa attacgttgc
    54301 cattatggtc agcaatgttg ccgctggtca gttccagttt atcagtacgg aaagcttcag
    54361 tatcgaactg atcaacattc acatccagca caccaccgtt agtcacagtg atagtattgg
    54421 tatacagatt agaataatta ttccaaccgg tagattcggt taaattaacg gtactgtagc
    54481 tatcaacagt caggtggtct gtagcaacca cgccatttgc accaatattc agtgaagaac
    54541 cgttggttaa accaatggta tctgatacca gagaggaatc agtaacattt acctgagagc
    54601 cattattt
//
LOCUS       DAJTNN010000007        47213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTNN010000007 DAJTNN010000000
VERSION     DAJTNN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.007"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            17..754
                     /locus_tag="OO150_000403"
     CDS             17..754
                     /locus_tag="OO150_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5514665.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGKASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            865..1875
                     /gene="pyrD"
                     /locus_tag="OO150_000404"
     CDS             865..1875
                     /gene="pyrD"
                     /locus_tag="OO150_000404"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5514666.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            2049..2591
                     /gene="zapC"
                     /locus_tag="OO150_000405"
     CDS             2049..2591
                     /gene="zapC"
                     /locus_tag="OO150_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5514667.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNVLVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2588..3697)
                     /gene="ycbX"
                     /locus_tag="OO150_000406"
     CDS             complement(2588..3697)
                     /gene="ycbX"
                     /locus_tag="OO150_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5514668.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            3941..6049
                     /gene="rlmKL"
                     /locus_tag="OO150_000407"
     CDS             3941..6049
                     /gene="rlmKL"
                     /locus_tag="OO150_000407"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5514669.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGALSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            6061..7968
                     /locus_tag="OO150_000408"
     CDS             6061..7968
                     /locus_tag="OO150_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5514670.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNIAEGKQEVMVNGKPRHVLSYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            8098..9351
                     /gene="pqiA"
                     /locus_tag="OO150_000409"
     CDS             8098..9351
                     /gene="pqiA"
                     /locus_tag="OO150_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5514671.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9356..10996
                     /gene="pqiB"
                     /locus_tag="OO150_000410"
     CDS             9356..10996
                     /gene="pqiB"
                     /locus_tag="OO150_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5514672.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            10993..11556
                     /gene="pqiC"
                     /locus_tag="OO150_000411"
     CDS             10993..11556
                     /gene="pqiC"
                     /locus_tag="OO150_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5514673.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11812..11979
                     /gene="rmf"
                     /locus_tag="OO150_000412"
     CDS             11812..11979
                     /gene="rmf"
                     /locus_tag="OO150_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5514674.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(12049..12567)
                     /gene="fabA"
                     /locus_tag="OO150_000413"
     CDS             complement(12049..12567)
                     /gene="fabA"
                     /locus_tag="OO150_000413"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5514675.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12636..14396)
                     /locus_tag="OO150_000414"
     CDS             complement(12636..14396)
                     /locus_tag="OO150_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5514676.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPNLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14582..15034
                     /gene="matP"
                     /locus_tag="OO150_000415"
     CDS             14582..15034
                     /gene="matP"
                     /locus_tag="OO150_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5514677.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(15110..16150)
                     /gene="ompA"
                     /locus_tag="OO150_000416"
     CDS             complement(15110..16150)
                     /gene="ompA"
                     /locus_tag="OO150_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5514678.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(16507..17016)
                     /gene="sulA"
                     /locus_tag="OO150_000417"
     CDS             complement(16507..17016)
                     /gene="sulA"
                     /locus_tag="OO150_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5514679.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            17235..17863
                     /gene="sxy"
                     /locus_tag="OO150_000418"
                     /pseudo
     CDS             17235..17863
                     /gene="sxy"
                     /locus_tag="OO150_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
     gene            complement(17826..19988)
                     /gene="yccS"
                     /locus_tag="OO150_000419"
     CDS             complement(17826..19988)
                     /gene="yccS"
                     /locus_tag="OO150_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5514680.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(19998..20444)
                     /locus_tag="OO150_000420"
     CDS             complement(19998..20444)
                     /locus_tag="OO150_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5514681.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            20567..22627
                     /gene="helD"
                     /locus_tag="OO150_000421"
     CDS             20567..22627
                     /gene="helD"
                     /locus_tag="OO150_000421"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5514682.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPLPVNRLEEFDNCREAWRK
                     CQAWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLA
                     GAGSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITART
                     FHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEE
                     MQWSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIK
                     LMAPLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAA
                     LLAALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNS
                     RIGKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERIL
                     ILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAAR
                     ESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPV
                     ARKP"
     gene            complement(22659..23117)
                     /gene="mgsA"
                     /locus_tag="OO150_000422"
     CDS             complement(22659..23117)
                     /gene="mgsA"
                     /locus_tag="OO150_000422"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5514683.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23213..23875)
                     /locus_tag="OO150_000423"
     CDS             complement(23213..23875)
                     /locus_tag="OO150_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5514684.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            24048..24461
                     /locus_tag="OO150_000424"
     CDS             24048..24461
                     /locus_tag="OO150_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5514685.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(24506..24823)
                     /gene="hspQ"
                     /locus_tag="OO150_000425"
     CDS             complement(24506..24823)
                     /gene="hspQ"
                     /locus_tag="OO150_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5514686.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(24881..26071)
                     /gene="rlmI"
                     /locus_tag="OO150_000426"
     CDS             complement(24881..26071)
                     /gene="rlmI"
                     /locus_tag="OO150_000426"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5514687.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            26166..26444
                     /gene="yccX"
                     /locus_tag="OO150_000427"
     CDS             26166..26444
                     /gene="yccX"
                     /locus_tag="OO150_000427"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5514688.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(26441..26770)
                     /gene="tusE"
                     /locus_tag="OO150_000428"
     CDS             complement(26441..26770)
                     /gene="tusE"
                     /locus_tag="OO150_000428"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5514689.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(26861..27520)
                     /gene="yccA"
                     /locus_tag="OO150_000429"
     CDS             complement(26861..27520)
                     /gene="yccA"
                     /locus_tag="OO150_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5514690.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(27727..27814)
                     /locus_tag="OO150_000430"
     tRNA            complement(27727..27814)
                     /locus_tag="OO150_000430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27778..27780),aa:Ser,seq:tga)
     gene            28241..29359
                     /gene="hyaA"
                     /locus_tag="OO150_000431"
     CDS             28241..29359
                     /gene="hyaA"
                     /locus_tag="OO150_000431"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5514691.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            29356..31149
                     /gene="hyaB"
                     /locus_tag="OO150_000432"
     CDS             29356..31149
                     /gene="hyaB"
                     /locus_tag="OO150_000432"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5514692.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            31168..31875
                     /gene="hyaC"
                     /locus_tag="OO150_000433"
     CDS             31168..31875
                     /gene="hyaC"
                     /locus_tag="OO150_000433"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5514693.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDGTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            31872..32459
                     /locus_tag="OO150_000434"
     CDS             31872..32459
                     /locus_tag="OO150_000434"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5514694.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            32456..32854
                     /gene="hyaE"
                     /locus_tag="OO150_000435"
     CDS             32456..32854
                     /gene="hyaE"
                     /locus_tag="OO150_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5514695.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            32851..33708
                     /locus_tag="OO150_000436"
     CDS             32851..33708
                     /locus_tag="OO150_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5514696.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            33842..35386
                     /gene="appC"
                     /locus_tag="OO150_000437"
     CDS             33842..35386
                     /gene="appC"
                     /locus_tag="OO150_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5514697.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            35398..36534
                     /gene="appB"
                     /locus_tag="OO150_000438"
     CDS             35398..36534
                     /gene="appB"
                     /locus_tag="OO150_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5514698.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADVRWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLSSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            36547..36639
                     /gene="cbdX"
                     /locus_tag="OO150_000439"
     CDS             36547..36639
                     /gene="cbdX"
                     /locus_tag="OO150_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5514699.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            36719..38017
                     /gene="appA"
                     /locus_tag="OO150_000440"
     CDS             36719..38017
                     /gene="appA"
                     /locus_tag="OO150_000440"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5514700.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNV
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(38132..40312)
                     /gene="etk"
                     /locus_tag="OO150_000441"
     CDS             complement(38132..40312)
                     /gene="etk"
                     /locus_tag="OO150_000441"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5514701.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLHDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(40332..40778)
                     /gene="etp"
                     /locus_tag="OO150_000442"
                     /gene_synonym="yccY"
     CDS             complement(40332..40778)
                     /gene="etp"
                     /locus_tag="OO150_000442"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5514702.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(40766..41905)
                     /locus_tag="OO150_000443"
     CDS             complement(40766..41905)
                     /locus_tag="OO150_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5514703.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTRNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(41951..44047)
                     /locus_tag="OO150_000444"
     CDS             complement(41951..44047)
                     /locus_tag="OO150_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5514704.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVNDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(44047..44793)
                     /locus_tag="OO150_000445"
     CDS             complement(44047..44793)
                     /locus_tag="OO150_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5514705.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(44790..45434)
                     /gene="gfcB"
                     /locus_tag="OO150_000446"
     CDS             complement(44790..45434)
                     /gene="gfcB"
                     /locus_tag="OO150_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR5514706.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(45541..45846)
                     /locus_tag="OO150_000447"
     CDS             complement(45541..45846)
                     /locus_tag="OO150_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HCR5514707.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(46288..46500)
                     /gene="cspH"
                     /locus_tag="OO150_000448"
     CDS             complement(46288..46500)
                     /gene="cspH"
                     /locus_tag="OO150_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5514708.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            46786..46998
                     /gene="cspG"
                     /locus_tag="OO150_000449"
     CDS             46786..46998
                     /gene="cspG"
                     /locus_tag="OO150_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5514709.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            47009..47197
                     /locus_tag="OO150_000450"
     CDS             47009..47197
                     /locus_tag="OO150_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5514710.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVTLQIRTDHAPLDFTK"
BASE COUNT        11627 a        11727 c        12404 g        11455 t
ORIGIN      
        1 cagtcgggta aagtgaatgc cagcgcgaca tttgtacttc attatgacta atacgtggaa
       61 cagattggcg ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg aattgcgggc
      121 aggagtggtg gtcggcggaa cgcgatttat ctttccggca gacagagaat cgatatctat
      181 tttactgact aataccagtc aggaatcctg gctgattaac agtaaaatca accgcccaac
      241 gcgttgggcc ggggggaaag cgtcgacagt gccagcacca ttactggccg ctccgccact
      301 tattctcctg aagcccggta ctacaggcac gttgcgcttg ctgagaacgg aaagcgacat
      361 cttgcctgtg gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc catccggcaa
      421 agttgaaaat cagagcgtaa aagtggcgat gcgctcggta tttaaactgt tctggcgacc
      481 cgaaggtttg ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga cacggaattc
      541 gcagggtgta caactcacta acccaacgcc ttactacatt aacctgattc aggtgagcgt
      601 aaatggtaaa gcgttaagta atgcgggagt tgtgccgcct aaaagccagc gtcagacaag
      661 ctggtgtcag gcaatcgcac cgtgtcatgt cgcctggcgg gcgataaatg attacgacgg
      721 gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac gtttgcgttt
      781 tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgc ccccggtttg
      841 cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc ccttttccag
      901 ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgtat tacaggaacg
      961 ccgtttgaag cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg catgggcctg
     1021 acgtttaaaa atccgcttgg tctggcagcc ggtcttgata aagacgggga gtgcattgac
     1081 gcgttaggcg cgatgggatt tggatcgatc gagatcggta ccgtcacgcc acgtccacag
     1141 ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt gatcaaccgt
     1201 atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa ggcccattat
     1261 gatggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtgga gcagggcaaa
     1321 gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat cgccatcaat
     1381 atttcatcgc cgaatacccc aggattacgc acgctgcaat atggtgaagc gctggatgat
     1441 ctcttaaccg cgattaaaaa taagcaaaat gatttgcaag cgatgcacca taaatatgtg
     1501 ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca ggttgccgat
     1561 agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact cgatcgttct
     1621 cttgttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg tccgcttcag
     1681 ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg cttaccgatc
     1741 atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc tgcgggtgcc
     1801 tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat taaagaaatc
     1861 gttacccata tctaattatt tcttcgaatt cctctttata agataatcag ggctttattt
     1921 tcagccctgg ttgttttata ttcatcgctg ttgcttattt agacattttg tacttttatt
     1981 gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct gcggttttat agggtagggg
     2041 agaggcagat gcgaattaaa ccagacgata actggcgttg gtattacgat gaagagcacg
     2101 atcgaatgat gctcgattta gccaatggta tgctatttcg ctcacgtttt gcgcgcaaaa
     2161 tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgacgacgcc gcgctctatt
     2221 tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa gccgaactgg
     2281 tgctgaatgt actggtagca attcgctatc tcaaaccgca aatgccgaaa agctggcatt
     2341 ttgtttccca tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc gtttggttga
     2401 gcgatactca cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat gccgcactat
     2461 gcctgctggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg ggcgacgcca
     2521 ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc agcctcgaac
     2581 aggcagtcta acgcgccaac ttaagtgcag tcttcggtac acagctacag caaagaatgg
     2641 tgccatcatc gcccattgct gattttttca gcggcgagac ttcgccttct aaaatctgaa
     2701 cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt ccctgatttt
     2761 ctaattgctc cagcaacacc tgttggttat ttccacgaaa tgcctgtccc tgccagtcaa
     2821 tatctacatt tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg gcagctgcgc
     2881 cgtaaatttt tgccggagcc gttgccagaa tttccacctc atcgccaacg cgaatcacgc
     2941 cgctattacg ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc tgggcagtgc
     3001 ggaaagattg caatgttttt aatggttcgc ctgccggatg tttttgccct ttttctgggc
     3061 tgacggtggt gaaaatacag cggctacaag gtttaaccac atcaaacacc acatcaccaa
     3121 tgcgaatcac tttccagcga tcttcttccc aggctgacgc gccggaaacc accagattgg
     3181 ggcggaattg ctccattttt acactggccg gacaacgttg ttggagatca cgtaacgagg
     3241 cttcgttagc aagaaggtaa ggatagccat cagcaaatga cagaggtaca gtgttgtggc
     3301 gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg cgggagaaaa
     3361 atccacttag ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa tgtgtgcccc
     3421 aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat gcgctactgc
     3481 catctggtgc ggtgagatgc aagccatcat gcacaggtga aggagtaaac cgtaccatct
     3541 ggggaaactg gcgagcggta ataaacgtac cgtcaggttc cgtgatcata aagatgcgat
     3601 cgaaggccag accactgaca tcagccagag catgtgtaag accaatgccg cgcatcgatt
     3661 taacaggatg aataaaaagc cgggttaatg tcgccacggt gcggtcctca aatgaaaata
     3721 agccctcaac tttatgacat gacgcgctta ttagctataa tgcgcaacaa ttttcttagc
     3781 gcctgtccca ttaggctatt ttatttgtca gtttggccct gggttgtgct cgaaatcctc
     3841 acgtacttgt gtacgctccg ttttctccgc gctgtccatg tccagactga ctgcaacaat
     3901 tacgcctact gcggtaggct cttagagtaa aagtgacgat atgaattctc tgtttgccag
     3961 tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg gggccgttga
     4021 atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg tttaccagag
     4081 cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt gtaaggttta
     4141 cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga tgtttaatcc
     4201 tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac accatccgca acagtcagta
     4261 cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa atctgccgcg
     4321 tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc ataaagaaac
     4381 cgccagtatc gctcttgatc tgagtggtga tggtttacat ctgcgtggct atcgcgatcg
     4441 tgctggtatt gcgccgatca aagaaaccct ggcagccgct attgtgatgc gatccggctg
     4501 gcagcctgga acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc tgattgaagc
     4561 ggcgatgctg gcgaccgatc gcgcacccgg cttgcaccgt gggcgttggg gctttagcgg
     4621 ctgggcgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc aaactcgcgc
     4681 ccgtaaaggc ctggctgagt atagctctca tttttacggt tcggacagcg acgcacgggt
     4741 gattcaacgt gcacgcacta acgcccgtct tgcggggatt ggtgaactga tcacctttga
     4801 ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg gcacagtgtt
     4861 gagcaacccg ccatacgggg aacgtctgga cagcgaaccg gcgctgattg cgctgcatag
     4921 cctgctgggt cggatcatga aaaaccagtt tggtggctgg aatctctctt tgtttagtgc
     4981 ctcgccggat ctgctaagct gcttgcagct gcgtgcagac aaacagtaca aggcgaaaaa
     5041 cggcccgctg gactgcgtac agaaaaatta ccacgttgcc gaaagcactc cagacagcaa
     5101 accggcgatg gtagcggaag actacgccaa ccgcctgcgt aagaacctca aaaaattcga
     5161 gaagtgggct cgccaggaag gaattgaatg ttaccgcctg tatgacgccg atctgccgga
     5221 atataacgtt gccgttgacc gttattccga ctgggtggtg gtgcaggagt atgcaccgcc
     5281 aaaaactatt gatgctcaca aagcgcgtca gcgtctgttc gatattatcg ctgcaaccat
     5341 ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac gccagaaggg
     5401 caaaaaccag taccagaaac tgggcgagaa gggcgagttt cttgaagtta ccgaatataa
     5461 cgctcacttg tgggtgaacc tgacggatta tctcgatact ggtctgttcc tcgatcaccg
     5521 tatcgcccgc cgtatgctcg gtcagatgag caaaggcaaa gatttcctca acctgttctc
     5581 ttataccggt agcgccaccg tgcatgcggg attaggcggc gcgctcagca ccaccaccgt
     5641 ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga atggcctaac
     5701 cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg aggcaaatga
     5761 acagtttgat ctgatcttta tcgatccgcc aaccttctct aactcaaaac gaatggaaga
     5821 tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac gtctgctgcg
     5881 tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg atctcgacgg
     5941 cctggcaaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct cccaggattt
     6001 cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa aggaatagta
     6061 atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc gcttctcgat
     6121 aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtgggccg caacggcgcg
     6181 ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag ggctggatga cggtcgcatt
     6241 atttacgagc aagatttgat tgtagctcgt ctgcaacagg atccgccgcg taacgttgag
     6301 ggtagcgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata tctgaaacgc
     6361 tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct caacgaactg
     6421 gcgaaggttc aggaacagtt ggatcaccac aacctgtggc agctggaaaa ccgcatcaac
     6481 gaagtgctgg cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct ttccggcggc
     6541 tggttgcgta aagcggcatt aggacgcgcg ctggtgagta atccgcgcgt gctgttgctt
     6601 gatgaaccga caaaccacct ggatattgaa accatcgact ggctggaagg gttccttaaa
     6661 accttcaatg ggacgattat tttcatctcc cacgaccgtt cgtttatccg caatatggcg
     6721 acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccagggaa ttacgaccag
     6781 tacctgcttg aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc cgagttcgat
     6841 cgcaaactgg cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg ccgtacccgt
     6901 aatgaaggcc gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga acgtcgcgaa
     6961 gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa gatcgttttc
     7021 gaaatggaag acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga tttttcggcc
     7081 caggttctac gtggcgacaa aattgtcctg attggtccga atgggtgcgg caaaaccacg
     7141 ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca cgttggcacc
     7201 aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga taaaacggtg
     7261 atggataaca ttgccgaagg taagcaagag gtgatggtta acggcaagcc acgccacgta
     7321 ttgagctatt tgcaggactt tctgttccat ccgaaacggg cgatgacgcc ggtacgtgcg
     7381 ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa accaagcaac
     7441 ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga actgctggaa
     7501 gaactgatcg acagctatca gggcacggta ttgctggtta gccacgatcg tcagtttgtc
     7561 gataacaccg ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg tcgttatgtc
     7621 ggcggttatc atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa acagcctgcg
     7681 gtgaaaaaaa ccgaagaagc cgccgcggca aaagcagaaa ctgtaaaacg cagcagtagc
     7741 aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct cgaagatctg
     7801 gaggcgaagc tggaagccct acagacgcag gtggcggatg cttccttctt cagtcagcca
     7861 catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga gctggagcaa
     7921 gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc gcaaaatagt
     7981 cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg tttatcaggc
     8041 atgaaaacca acactttgtt agtcatctgt aaaatagcgc atcattaagg agtaccaatg
     8101 tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat gctggtggcg
     8161 ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac aacgttaacc
     8221 gtggcgtggg atgcccctcg gcagcgtccg accgcctatg cgttggctgc actgttcatg
     8281 ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt taccagtgaa
     8341 attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag cctcggcacc
     8401 tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat tctgttactg
     8461 gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt gctttttcaa
     8521 ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag tttcgttaaa
     8581 ctgatggctt acggcagcat tggggtaggc agcagctttc tcccctggtg tttattttgt
     8641 gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga cgacatcgcc
     8701 ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca cggggatacg tcaggggctg
     8761 cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg cccgcgttgt
     8821 agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc gctgcttgta
     8881 acgtccatca tgctgtatct tccggctaat attttgccca tcatggtgac ggatttatta
     8941 ggctcgaaga tgccgtcgac gattctcgct ggggtcattc tgttatggag cgaaggctct
     9001 tatcccgtcg ctgcggtgat ctttctggcc agtattatgg tgccaacgtt aaagatgatc
     9061 gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca agcgcgacag tgaaagaatg
     9121 catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga cgttttcgtt
     9181 atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta tccggcaatg
     9241 ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat gacgtttgac
     9301 ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg acagtatgga
     9361 atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg tgtggatatt
     9421 tcctatcgtc acggcgctca ttggggcctg ggttctattt tatcattaca gccatcaggg
     9481 accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca aaaccaccat
     9541 taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg atgatttgac
     9601 gcacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc ataaagacac
     9661 cgtcttttgg gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc tgggaacgct
     9721 gctgtctgga gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa tggataaata
     9781 cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc gtgtgattct
     9841 cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc gtggctatcg
     9901 ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca gttatcaact
     9961 gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgcttct ggaaagatag
    10021 tggcattgcg gttgatctga cgtcagcggg gatgcgtgtg gagatgggct cattgacaac
    10081 gctgcttagt ggcggtgtta gctttgatgt gccggaaggt ctggatttag ggcagccagt
    10141 ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg attcgttgta
    10201 caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc tgcaaccggg
    10261 agctccggta gaattccggg gtattcgcct gggtaccgta agcaaagtgc cattctttgc
    10321 gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtactga ttcgtatcga
    10381 gccagagcgg ctgaaaatgc agcttggcga aaatacggat gttgttgagc accttggcga
    10441 attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca ctggtgcact
    10501 gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc gtgaatttaa
    10561 tggttatcag attatcccga ctgttagcgg cggcctggcg caaatccagc aacgactgat
    10621 ggaagcgttg gataagatca acaaactgcc attgaatccg atgattgaac aggcaaccag
    10681 tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg atagcatgaa
    10741 caagatcctc gctagccagt cgatgcagca gttgccgacg gatatgcagt caacgttgcg
    10801 tgaattgaat cgcagcatgc agggcttcca gcctggctcc gcagcctaca acaagatggt
    10861 ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc tgaaaacgct
    10921 caatgagaag agtaacgcgc tggtatttga agcgaaggac aaaaaagatc cagagccgaa
    10981 gagggcgaaa caatgaaaaa gtggctagtg acgattgcag cactgtggct ggccggatgc
    11041 agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag cggtacacaa
    11101 agtaccgcca gccagggcaa tcgtctgtta tgggtagagc aggtcactgt tcctgactat
    11161 ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat tgccaacaac
    11221 aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc caacctgagt
    11281 acgcaactgc ccggctgggt ggttgcctcc cagcctctgg gaagcgtcca ggacacgctc
    11341 aatgttaccg taacggagtt taacggtcgc tatgatggca aggtcattgt cagtggtgag
    11401 tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga aggagtgcaa
    11461 actcaggatg gttacgatga gatggttaaa gtgctggccg gtgtctggag tcaggaagcc
    11521 gcttctattg cacaagagat aaagcgtcta ccttaattat aaagatttgt aagtataacc
    11581 gtctccggta tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa aatttgttcc
    11641 tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga cagtggcgtg
    11701 aattttgcgc attgacggca gttatgattc gcggtattgc ttaactgtga ttgcacattt
    11761 agtaatcact gttttctttt ccaccagaaa ccagtatgag ggaaacgagg catgaagaga
    11821 caaaaacgag atcgcctgga acgggcacat caacgtggtt atcaggccgg catcgccgga
    11881 cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg gctgggaggc
    11941 tggcgagaag ccatggcgga cagggtagta atggcctgat tctgtctctt taaaaagaaa
    12001 cctccgcatt gcggaggttt cgccttttga tactctgtct gatgataatc agaaggcaga
    12061 cgtatcctgg aacagaccga ctttcaggtc gctggcggta tagatcagac gaccatcaac
    12121 cagcacttcg ccatctgcca gacccataat cagacgacga ttaacaatgc gtttaaagtg
    12181 aatacggtag gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt tcacttcgcc
    12241 aacgcccagc gcgcggcctt taccttcgcc gcccagccag ccgaggtaga accctactag
    12301 ctgccacatt gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa tgaagtggca
    12361 tccgaagaac cacagatccg gattgatatc cagttctgct tcaacatacc ctttgtcgaa
    12421 gttaccaccc gtttcggtca ttttgaccac acggtccatc atcagcatgt tcggtgctgg
    12481 caattgcggg cctttagcgc caaacagttc accgcgacca gaggcaagaa ggtcttcttt
    12541 tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc cttattttat tgaagcacgc
    12601 aggatagcta acacgtgtac gctgaacaag tccgatcagt tcggaataaa ccagttcagc
    12661 caacgtaatg gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg cgcgatacgt
    12721 tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat cccacaccag atttaataat
    12781 aacggcagtg cgtcagtcac atcgtctact gcccagatgg tgaatttgcc ttcttctacc
    12841 gctttcacca gttcactgtg aagacttaaa tggcgaacgt tagctgtggg gataatgaca
    12901 ccttgtttcc cggttaactc acgttgctgg caaatagcaa agaagccttc gattttctca
    12961 tttaaaccac cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt gatagcgata
    13021 ctctgattca ccggcacatc ggcgagggcg cttatcaggg cgcagagttc agccatcgag
    13081 gcactatctc catcaacttc actgtatgac tgctcaaatg tcagcgatgc tgagaagggg
    13141 atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat catccctttc
    13201 gcatggatat tgccgccaag ttccgctttg cgttcgatgt cggtgaattc accatcgcca
    13261 atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg acccggaaat
    13321 tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc aatcaggatt
    13381 tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg ccattcacgc
    13441 tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca cagggaggcg
    13501 acctctttac actggcggag gatccactgc gggctaagcg gaagtgtttc ttgttcaccg
    13561 gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg ggcaggtaag
    13621 tgattatgtc tggcggtaaa tgtcacccag cgacaccact ggcttactga ctccgcatcg
    13681 acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag ctctggctcc
    13741 atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt cagcttcaat
    13801 ggcatcgaag gcacagagac agggagaggg cgcgactcat caaacgcaac ccagtcaaaa
    13861 cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc cagcagtgta
    13921 cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg ctgcagggta
    13981 atgtcgccat taaactggcg caggcagcca aagagttgct ccgcttctac ccagtcggca
    14041 gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg acgtaagcgg
    14101 atggagtggc cggaaacctc atagtgatcg cccaccagtt ggcctgcatc gctttgtagc
    14161 gtacgcgcgg catcggcaat aagattcaga tactcagact cttccggggc cttagccagc
    14221 ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg cagccgcggt
    14281 tgagtatcac tgaataaagg atcgttttcg tcaatcaaat ttggctgagc aaatatttcc
    14341 tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat ggtcaaagtt
    14401 gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact acagcacacg
    14461 tagaaagcac cgacaatact cctggcatgg gcgttaaagc tcacaggatg gagattcttt
    14521 tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca ttgagatcgc
    14581 tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt atctggtgaa
    14641 gaagcatcgc gaaggggagt taattacccg ttacatagaa gccagtgccg cccaggaagc
    14701 cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg gtaaatggct ggattgacaa
    14761 gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc attcgggcca gacggaagcg
    14821 ccattttaat gcggaacacc agcatacgcg caaaaagtcg atcgatctgg aatttattgt
    14881 ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg aaacgattgt
    14941 tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt cttctctgaa
    15001 gctggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt caatttacta
    15061 aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgataact taagcctgcg
    15121 gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga tccggagcca
    15181 ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt
    15241 tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg gagatcaggt
    15301 aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg tcagaaccga
    15361 tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg ttgctcagct
    15421 ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct ttgttgaagt
    15481 tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt gccggagctg
    15541 gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa acacccaagc
    15601 tcagcatgcc gttgtctgga cgagtgccga tggtgtgtgc gtcaccgatg ttgttggtcc
    15661 actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca ccgccagcga
    15721 agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta gtgtctgcac
    15781 gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt gggtaaccca
    15841 gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg ctgcctttgt
    15901 acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg ttaacctggt
    15961 aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg ttgttgatga
    16021 aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag gtgttatctt
    16081 tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc gcgatagctg
    16141 tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat ctccaacgag
    16201 ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac cgatatcttc
    16261 ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa acttacaagt
    16321 gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta atcttttttg
    16381 tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa
    16441 aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga ttaatcctaa
    16501 atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc tcgtggcgtg
    16561 agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat ttgccgcatc
    16621 aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga tcaccacact
    16681 gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag gggagagctg
    16741 gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt cccgactcag
    16801 tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa
    16861 tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa cttcactgat
    16921 aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg cggatgagaa
    16981 cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc ctgtgagtta
    17041 ctgtatgaat atacagtaca tccagtgaca acaaagatca accctatttt cggaaagagc
    17101 ctcgcaaatt ttttcgttgg tgacgggaaa acataaatta atcttgcccc ttaagaataa
    17161 gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta tgttatgatt
    17221 ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca caagaatacc
    17281 tggcaatgtt gggcacaatt gaataccgat cattgtttgg cagttacagc ctgaccgttg
    17341 acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa
    17401 gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag tgtggccgat
    17461 ccgttaccct taattactat cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg
    17521 tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc
    17581 gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt ctcggggagt
    17641 ggggattaag gatgtacggg cgttacgtat acttggggca aaaatgtgtt ggttgcgact
    17701 gcggcagcaa aacagtctgg tgacagaaaa gattctgttt atgcttgaag gtgccattat
    17761 cggcattcat gaagctgcgc tcccggtggc acgccgccag gagcttgcag aatgggctga
    17821 ctcacttacg ccgaaacagg agtttcctgc ggaacttgag taatctggcg ttgcagacga
    17881 ccaatctcag gcagtaatgc aatcaataat ccaacttgtt gtacgaccag aggttctttg
    17941 ctgtctgccc gtggttcaag ttgctgcatc cgctgtttca ggctagctaa tgcctcattg
    18001 acgcgttctt catcagcagg ttgatgatgt aacgcgtcat caacatagca aactgcgtca
    18061 tcaagaaacg ccagaatttc aggattagtt aactgctccc ggtgagcacc gagggctgag
    18121 atatagctgg taaacgtatg gttaaggcac agcaaccgaa acgcggcttc gcgaatttgc
    18181 ggggtaacgt tcggctcgct ggacatattt gataccaccg acgccagctc agcatcacgg
    18241 ttgtgtgcat cgcggcgggc aatacgatac gccagacggt tatcacgccc ctgatggtat
    18301 tgctccagta tggcatcgag ataccgacag ttggcctctg tggcgcgttc gagcatgcgc
    18361 ggcaaattgc gaaactgcca gtcaggccag atgtagctca ctgccgccca cgcaatggca
    18421 caaccaatca gcgtatcgat tacgcgaggt aacgctactt caaaaccttc acccagtaag
    18481 ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg catgagcgta ttgcacgtta
    18541 cggaaggcaa aaaagagcac gccggtaata accagcagca ccagctgccc ttccagtgat
    18601 ggcacaaacc acagcacagg aatgccaatg gcgataccta ccagcgtacc aataatcctt
    18661 aacttcaggc ggtggcgcgt ggcgttatag tttggctggc agacaaacaa acttgtcagc
    18721 aagatccaaa acccgtgatg cattccggtt atttgaatga tggcgtagcc gaagcacaac
    18781 accagcgaca ttcttaccgc atgacggaag agggcggatt ccggcgtgaa gtgacggcta
    18841 agacgcagcc agatatcact caacccgtgc gggctgtcat cagcgagctc attttcgtca
    18901 ttattatggg gtagtgcctg ggcctgttct gattcaattg ttgccagttg ggcatcaatg
    18961 gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta aatcggcggg tgcgccgtta
    19021 tcgcgcatcc gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa atgcggatca
    19081 tgttgataag gctgacgcaa caaaatacag cgtgacagtt gctggcacgc ctggccctgc
    19141 atcgacatca gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg ttcacgcaat
    19201 gtttgatact gaatatgaga agagctggca cgctcgtgaa tatcctgtgc gacaaagtaa
    19261 taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg ggtcagcagc
    19321 gagagtttcg tctgattcaa tgtcgccatc agcagaccgt tggcgagagc caaatcgtac
    19381 agcggtgcct ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt gagctcaaga
    19441 taacgcgcca gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg gaccgggaac
    19501 agcagatgac caataagtgt caggacgttg taccagacgg caccggccag cagatacatc
    19561 ggctgctgat accagtgctc atacagtgat gttcccaaca tagtgtaaat ggcgatcagc
    19621 aatgcaccga aggcaattgt tgcatagcgt tgacccagac cgccgagcaa aatgaagccg
    19681 ctggtagaga gcgttaagcc aatcgcaaat agccagggcc agggaaacag caattctact
    19741 gaggccgagg cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa acgtcccgcc
    19801 agtcggtcat cgagatcggt cagcgccgct gccaccatcc ccagcgttag cggaatcgtc
    19861 agttttacat cacccagcca ccacggaaac gctgtggttc cacaaagcgc aataaaaata
    19921 cgcgcgtaat acagccaggc gctgttccag gtatagcgtt tgagcaaagg acttagcata
    19981 aaggccatat tagtggatta ttcaaaacga cgacgtgcat tggcttcacg cgcagcttgc
    20041 gctgtttcta ccgataccac gcgacgacca accggccata gtgcaatagc ggcaatttta
    20101 aagttcgcaa tgccgacagg aatgccaatg attgaaatac attgtgcgat gcccgttgca
    20161 atgtgcatca ggcataacca ccagccaaag aaaatcagcc agaaaatatt caataccgta
    20221 ccgccagtat tcagcagcac atttttgcca gccgggttca gttcatcgac atgaatagct
    20281 tcattgccat aaggcaccag agacagttta gtgatctccc agcaggatcg tgtcagcggt
    20341 aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca gccagcccag agtggtggca
    20401 aatccgccaa gcacaaagtt cagaatgttc aaaacggtac gcataaaacc tcgctttact
    20461 gtggttttca gtaatgggcg gcaattgtaa cgtttttttg ggctggagca cgttttctct
    20521 gacggttaca ctgataagaa ataatttcgt gtggatctac agagtcatgg aactgaaagc
    20581 gacaacgctt ggaaaacgtc tggcacagca cccttacgat cgggcggtga tcctcaatgc
    20641 cgggattaaa gtctccggcg atcgccacga ataccttatt cctttcaatc aattactggc
    20701 gattcactgt aagcgtggtc tggtatgggg cgagctggaa tttgtactgc cggacgaaaa
    20761 agtggtgcgt ctgcacggca ccgaatgggg cgagacgcag cgtttttacc atcatcttga
    20821 tgctcactgg cggcggtgga gtggcgagat gagcgaaatt gcgtctggtg ttttacgcca
    20881 gcaactggat ttgattgcca cgcgcactgg agaaaataaa tggctgacgc gtgagcaaac
    20941 ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg ttgccgttgc cgttgccggt
    21001 taaccgactg gaagaattcg ataactgccg tgaggcgtgg cgtaaatgtc aggcctggtt
    21061 gaaagatatt gaaagcgctc ggttgcagca taaccaggcg tataccgaag ccatgcttac
    21121 cgagtatgcg gattttttcc gccaggtcga gtcttcaccg ctgaatccgg cgcaggcccg
    21181 ggcagtcgtt aatggcgagc attctctgtt agtgctggca ggtgcaggaa gcggaaaaac
    21241 gtcggtgctg gtggcccgtg caggctggtt gctggcacgt ggtgaagcgt cccctgagca
    21301 aattttattg ctggcgtttg gtcgcaaagc cgctgaagag atggacgagc ggattcgcga
    21361 acggctacat accgaagaca ttaccgcacg cacgtttcat gcgctggcgc tgcatattat
    21421 tcagcagggc agcaaaaaag ttccgatagt cagcaaactg gaaaatgata ccgctgcccg
    21481 tcatgaactc tttattgctg agtggcgcaa gcaatgcagc gaaaagaaag cgcaggcgaa
    21541 gggctggcgg caatggctga cggaagaaat gcagtggtca gtgccagaag gtaacttctg
    21601 ggatgatgaa aaattacagc gtcgccttgc ctctcgcctc gatcgctggg taagtctgat
    21661 gcggatgcac ggtggtgcac aggcagaaat gattgccagt gcacccgaag agattcgcga
    21721 tctgttcagt aaacgtatca agttgatggc cccgttatta aaagcctgga aaggtgcgct
    21781 gaaggcagaa aacgctgtcg atttttcggg ccttattcat caggcgattg tgattctgga
    21841 gaaaggtcgc tttatcagcc cgtggaagca tattctggtt gatgaatttc aggatatctc
    21901 gccgcagcgg gcagcgttgt tagcggcatt acgcaagcaa aacagtcaga cgacgttgtt
    21961 cgctgttggt gatgactggc aggcgattta ccgattcagc ggtgcgcaaa tgtcgctcac
    22021 caccgctttc catgaaaact ttggtgaagg cgaacgttgt gatttagaca cgacttaccg
    22081 ttttaacagt cgtatcggta aggtggcaaa ccgttttatt cagcagaacc caggccagct
    22141 gaaaaagccg ctaaacagct taaccaatgg agacaaaaaa gccgtcacgt tattggatga
    22201 gagtcaactt gacgctttgc tggataagct ctctggttat gccaaaccgg aagagcgcat
    22261 tctgatcctg gcgcgttacc atcacatgag gcctgccagc ctggaaaaag cggcaacacg
    22321 ctggccgaag ttgcaaatcg actttatgac cattcatgcc agcaaagggc aacaggcgga
    22381 ttacgtcatc atcgttggct tgcaggaggg aagtgatggt tttccggctg cggcgcggga
    22441 gtcgattatg gaagaggcgc tactgccacc ggttgaggat ttcccggacg ctgaagaacg
    22501 gcggttaatg tacgtggcgc tgacccgggc acgccatcgg gtatgggcac tgtttaacaa
    22561 agagaatccc tctccctttg ttgaaatact gaaaaatctg gatgtgccgg tggcgagaaa
    22621 accgtaagaa acaggtggcg tttgccacct gtgcaatatt acttcagacg gtccgcgaga
    22681 taacgctgat aatcggggat cagaatatcg accgcgtcgt tgaaatgcgg cgactggatt
    22741 atgaagtctg ccgttgccac gttggtggcg accggaatgt tccataccgt cgccagacgc
    22801 agcaaggctt tcacgtcagg atcgtgcggc acggcattta gtggatccca gaagaaaatc
    22861 aatacatcaa ttttcccttc tgagatcaat gcgccaacct gctggtcacc ccccattggg
    22921 ccactcaaca tcgcgttgac gttcatgccg gtcgcgcggg aaattaagtt accggtagtg
    22981 cctgttgcat acagtacgtg ttgttccagt aacggttgat gccgttccac ccagctcatc
    23041 agcatttgtt tgcagtgatc gtgtgccacc agcgcaatat gtttccgcgc aggtaaagtg
    23101 cgagtcgtca gttccataat gtacatccgt agtcaacttt cctacagatt actgtaagca
    23161 cttatcgctg caagataaag accgaaaaag cctgcgcaca ggcacaaaaa tctcaggaag
    23221 atggttgttt ttccgcccac tgcaggaaag tatttcgcgt ttgtgggtca gccagtttaa
    23281 accaatactt cagccgttgt tctgtgagca cctgagactg cggtggaata gcatccagct
    23341 cggaaacacc cgaaagcagc gtactgtcat ctgccatcat cgtggcaaat tgcggtagtg
    23401 aagcgcgttt cgcggatttg ttatagcgtt caacctcgac ctcataatca atcgtttttg
    23461 ccgttgaggt aatggcgaga atatccagct ttaccggaat cggcgtcgca tcgccatcca
    23521 acaattcaag gcgcggcgcg gcatcaaaat ggttagcttc ccgctcattc tccaggcgag
    23581 gcaggcgaaa attgacctgg ttgatgagct gggtattaaa actgaccact agcggtgggg
    23641 agatatacag ccgttcttca ctattggaaa gatgaatcgt cttctcaacg cgaaacacta
    23701 actgatgcgg tccattatcc agttcaatgc tgtccgcacc gcgcagcaga gagctggaaa
    23761 ccttcttgcc gtcgagtacc agaagatcga catcggttga aagccgcagc gtggtggcaa
    23821 aaacggatac cggcaaacat aatgcaatca aggtggtcac gatgccggtt ttcataggta
    23881 gctcctgtca aaagaccgcc cgaataataa tgtgtcaaaa tttttcttta atcatactta
    23941 ctaacatata gacatatttc ccgcttttgc tctcattcat tcgtattagc tgcatggttg
    24001 gcatgtcggc tttgtcgtac actttgttaa acagtctgga ggaaataatg aaagaaaccg
    24061 atattgcagg cattttaacg tccactcaca ccattgcgct ggtgggggca agcgacaaac
    24121 ccgatcgtcc cagctatcgc gtgatgaaat atctactcga ccaggggtat catgtcattc
    24181 cggtttcgcc aaaagttgcc ggcaaaacgc tgctgggcca gcaggggtat ggcacgctgg
    24241 cagacgttcc ggaaaaggtc gatatggtcg atgttttccg taattcagaa gcggcgtggg
    24301 gcgtggcaca ggaagctatt gccattggcg cgaaaaccct ctggatgcaa ctgggggtga
    24361 ttaatgagca agcggcagta ctggcacggg atgccgggtt gaatgtggtg atggatcgct
    24421 gcccggctat tgagatccct cgcctgggcc tggccaaata aaaaatcccc ggaaggcaaa
    24481 aaccttccgg ggattcgttc agggatcagt tacgcagacg cggggcctgg agttgtttgc
    24541 ggatggtctg cgccagctca tccatggatg gctgttcagg atgttcatcc tgcaattcgc
    24601 tacttagctg ggcttcagcc aggtaggtat gaaccggtag gccgttatcg tcctccatca
    24661 ctacgtgata ccacggagcg gcgcgaagct cgtcatttac cgccagctca tcaggcgacg
    24721 gttcagagag cgaataaacc gggtcgatat ccacgaccac tccgagataa cctaacaggg
    24781 aatggcggac ctgctggccg ataccgaatt tgctggcaat catagtcacc tcccgggaaa
    24841 tctttacctt tgatataagg gtaaaattcc acatttcaag ttacatgacg cgacaggcaa
    24901 accctttcag atatagccct tccggatagg tagcgatcac cggatgatcg gctgcctgac
    24961 ggaactgctc tataaattgt acatcacggc cggcatcaat tgcggcatcc gcgatgattt
    25021 tctgaaataa atcgctggtc atcagaccgg aacaggagaa agtcaggaga ataccgcctt
    25081 cattcagcaa ctgaatcgcc agcatgttga tatctttata gccacgacac gcgcccatca
    25141 actggctttt attctcaaca aacttcggcg ggtccatcac gataacgtca aatttttcac
    25201 cgcgatcgcg ataagtacgc agcaatttaa agacatcatc acggacaaac tcagccttgc
    25261 tcagatccag tttgttcagc tcaacgttct gccgtgcaat atccagcgct tcctgggagg
    25321 tatcaacgct gacaacctgg ctgcaaccgc ccatcagtgc cgatacggcg aaaccaccgg
    25381 tataggagaa acagttcagc acacgtttat tttcaacgta gcggcgggta gccaggcggc
    25441 tgtctcgctg gtccaggtag tagcccgttt tgtgtccgtg ctgaatatcc accagcagct
    25501 tcatgccgtg ttcttcaatc ggcagcaggg caggcggcaa ctcgccggtg acgaggccca
    25561 gggtcagctc catcccttct tttttacgta ccgcaacatc gctgcgatcg taaatcgcac
    25621 attccgggta cagcgtttgc agggcactaa ctaatgccgc gcgctgatat tctgccccag
    25681 cactcagcag ttgcagcacc agaaaattac cgaaacgatc gatagtaata cccggcaggc
    25741 catcagattc tccggcgatt aaacgatagc tgtcgaggcc atctttttgc gccagccagt
    25801 cacgccattt ttgtgcttgt tgcaaacggc gggaaaaaaa agcaatgtcg atagactcag
    25861 acgggtcaaa cgtccagacg cgcgcccgga tttgcgaagc tggcgaataa gcgccgcgtg
    25921 ctaaccattt tccctgatga tcaacaatat cgatggtttc accgaggctg gctttacctt
    25981 ccatgcgggc aacggccccg gaaaagaccc acggatggcg acgaagtaat gatttttcgc
    26041 gccctttggc taacactaaa cgtacactca taatttactt ttctgtcgat gcaaagaaat
    26101 gggcgtcatt gtccggagtt ttccatggat ttgcaacgcg tccattaagg ataaggagag
    26161 cagcaatgtc gaaagtctgc ataattgcct gggtttacgg gcgagttcag ggcgtaggat
    26221 ttcgctacac cacacagtac gaagcgaaaa gactgggttt aaccgggtac gccaaaaatc
    26281 ttgacgatgg cagcgtggaa gtggttgcct gcggtgaaga agggcaggtg gaaaaattaa
    26341 tgcagtggtt aaagagtggc ggcccgcgtt ctgcgcgtgt agaacgggtg ctcagcgagc
    26401 cgcatcatcc ctcgggggaa ttaaccgatt ttcgtatccg ttaaatacat tttaccggtt
    26461 ttggcaggcc agcaattttg gtggcttgct tagccggacc tttcggaaac agtcggtata
    26521 aatagcggct gttacctttt tcttcgccaa atttattcgc catcgctttt accagcatac
    26581 gaatcgccgg agaagtattg aattccagat agaaatcacg cacaaaacgc accacttccc
    26641 agtgttccgg tgacagcgaa atcccttcgt tctctgcaat caccactgcc agcggctcac
    26701 tccactggct gctttctttg agatagcctt cggtatccgt ttctatctct ttaccttcga
    26761 agatcagcat aattattact accttaatca gactggcggc agtgtaacaa aaaacaaagc
    26821 cccgcataaa gcgaggctat gaaagtgtta gcgggtgaga ttaatcgcgg ctggcgaagc
    26881 ccagaatgct cagcaggctg acgaagatgt tgtacagcga aacatacagg ctaaccgtgg
    26941 cacgaatata gttcgtttca ccgccatgaa cgatgttgct ggtttcaaac aaaatagcgc
    27001 cagaggagat caaaatgaag accgcgctga tcgccagatg cagagcaggc agctgcagga
    27061 agatattagc aaccatacca atcagcacca ccacaatacc cgccatcagc ataccgccga
    27121 ggaacgacat atctttgcgg gtggtcagca catatgcaga gcagcagaag aacactaacg
    27181 ccgttccgcc cagagccata gcgattacgt cacccattcc ggcagacaga taggtgttca
    27241 gaataggtcc gaggatataa cccagaaaac cggtaaaggc gaatgcggag ataatcccgg
    27301 tcggcttatt cgccgtttta taggtcagga acatcaaacc atacataccc accagcgtca
    27361 gaatcagacc cggagatggc agcatcagca ccgtactggc agttgcggta atcgccgaaa
    27421 aggccagcgt caggctcagc agaaaatagg tattacgcag caccttatgg gtgctaagca
    27481 gtgatgtacg gtcatgtgaa gaactaacaa tacgatccat tgagtcactc tctatgacag
    27541 atgtaattaa ttaagcagca taatgataat gcgtaagggc acccagaagt tttacccatc
    27601 tttacgcatt tgatctggaa caggtttaac agcggattat caggtcatta agcaaatata
    27661 acgccctgag aatttcgaca ggcaaaagaa aaaggggtta gcatttagct aaccccttat
    27721 cttatttggc ggaagcgcag agattcgaac tctggaaccc tttcgggtcg ccggttttca
    27781 agaccggtgc cttcaaccgc tcggccacac ttccggaatg acgcgcacta taaacatccc
    27841 gatgcggcgt gtaaacccct aatttgtttg tttgcctgaa aaacagccaa aagtgcattg
    27901 atagcgtgaa ataacagcag attgatcatt tcatcaccat gaattccttc tcttttactc
    27961 gtttagcaac cggctaaaca tccccaccgc ccggcaaaaa gaaaaatagg tccattttta
    28021 tcgctaaaag ataaatccac acagtttgta ttgttttgtg caaaagtttc actacgcttt
    28081 attaacaata ctttctggcg acgtgcgcca gtgcagaagg atgagctttc gttttcagca
    28141 tctcacgtga agcgatggtt tgccttgcta cagggacgtc gcttgccgac cataagcgcc
    28201 cggtgtcctg ccggtgtcgc aaggagaaga gacgtgcgat atgaataacg aggaaacatt
    28261 ttaccaggcc atgcggcgtc agggcgttac ccggcgcagc tttctcaaat attgtagtct
    28321 ggctgccacg tcgctgggat taggcgcggg aatggcacca aagattgcct gggcgctgga
    28381 gaacaaaccc cgcattccgg tagtatggat ccacggtctg gaatgcacct gctgtaccga
    28441 atcttttatc cgctccgctc acccactggc gaaggacgtc atcctttccc tgatttccct
    28501 cgattatgac gatactttga tggctgccgc cggaacccag gcggaagaag tcttcgaaga
    28561 catcatcacg caatacaatg gcaaatatat cctcgcagta gaaggtaatc cgccgctggg
    28621 cgagcagggg atgttctgta tcagcagcgg tcgaccgttt attgagaaac tcaaacgtgc
    28681 cgctgccgga gccagcgcga ttatcgcctg gggaacctgc gcgtcctggg gctgcgtgca
    28741 ggccgcgcgg cccaatccga cgcaggcaac gcctatcgac aaagtcatca ccgacaaacc
    28801 cattatcaaa gtacctggct gcccgccgat cccggatgtg atgagcgcca tcattactta
    28861 catggtgacc tttgatcgct tgccagatgt cgacagaatg ggccgtccgc tgatgttcta
    28921 tggtcagcga atccacgata aatgctatcg ccgcgcccac ttcgacgccg gagagttcgt
    28981 ccagagttgg gatgatgacg ctgcccgcaa aggttactgc ctgtacaaaa tgggctgcaa
    29041 agggcctacc acctataacg cctgttcctc cacacgctgg aatgatggcg tttctttccc
    29101 aatccagtct ggtcacggct gcctgggctg tgcggaaaat ggtttctggg atcgcggttc
    29161 gttctacagc cgcgtggtcg atattccgca aatgggtact cattccaccg ccgataccgt
    29221 cggtttaacc gcgcttggcg tggtggcagc ggctgttggt gtgcacgcag tcgccagcgc
    29281 cgttgaccag cgcagacgtc ataaccagca acctacagaa accgaacatc agccaggcaa
    29341 tgaggataaa caggcatgag cactcagtac gaaactcagg gatacaccat caataatgcc
    29401 ggacgccgcc tggtggtcga cccgattacg cgcatcgaag gccacatgcg ctgcgaagtg
    29461 aatattaacg atcagaatgt gatcaccaat gccgtctcct gcggcaccat gtttcgcggg
    29521 ctggagatca tcctacaagg gcgcgacccg cgcgatgcgt gggcgttcgt tgaacgtatc
    29581 tgcggcgtct gtactggcgt acacgccctg gcttcggttt acgccatcga agatgctatc
    29641 ggtattaaag tgccggacaa cgccaatatc atccgcaaca ttatgctggc aacgctctgg
    29701 tgccacgatc atctggtgca cttctatcag cttgccggga tggactggat cgatgtgtta
    29761 gatgcgctga aagccgaccc gcggaaaacc tccgaactgg cgcaaagtct ctcctcttgg
    29821 ccgaaatcat cccctggcta tttcttcgac gtacaaaacc gcctgaaaaa atttgttgaa
    29881 ggcgggcagt tggggatctt ccgcaatggc tactgggggc acccgcagta caaactgccg
    29941 ccagaagcta acctgatggg ctttgcccac tatctcgaag ctctcgattt ccagcgtgaa
    30001 attgtcaaaa tccacgcggt ctttggcggt aaaaacccgc atccaaactg gattgtcggc
    30061 gggatgcctt gcgccatcaa cattgacgaa agcggcgcgg tcggggcagt caatatggaa
    30121 cgcctgaacc tggtgcagtc aattatcacc cgcacggcgg acttcattaa caacgtgatg
    30181 atccccgacg ccttagccat cggtcagttc aacaaaccgt ggagcgaaat cggcactggt
    30241 ctttctgata aatgcgttct cagctacggc gcattcccgg atattgccaa cgactttggc
    30301 gagaaaagtc tgctgatgcc tggcggcgcg gtgattaacg gcgacttcaa caatgtgctg
    30361 ccagtggatt tggttgatcc gcagcaggtg caggagtttg tcgaccacgc ctggtatcga
    30421 tatcccaacg atcaggtcgg gcgtcatccg ttcgatggca tcaccgaccc gtggtacaac
    30481 cccggcgatg tcaaaggcag cgataccaac attcagcagc tgaatgaaca ggaacgctac
    30541 tcgtggatca aagcgccacg ctggcgcggt aacgcgatgg aagtggggcc gctggcgcgc
    30601 acgttaatcg cttatcacaa aggcgatgct gcgaccgttg agtcggtcga tcgcatgatg
    30661 tcggcgttga acctgccgct ttccggtatc cagtcaacgt taggccgcat tttgtgccgc
    30721 gcgcacgaag cgcagtgggc cgcaggtaag ttgcagtatt tcttcgacaa gctgatgacc
    30781 aacctgaaaa acggcaatct cgccactgct tccacggaaa aatgggaacc tgcaacctgg
    30841 ccgacagagt gccgtggtgt cggttttacc gaagcgccgc gcggggcgtt aggccactgg
    30901 gccgccattc gcgatggcaa gattgatctc taccagtgcg tggtgccgac cacctggaac
    30961 gccagcccgc gcgatcccaa agggcagatt ggcgcttatg aagcggcgct gatgaacacc
    31021 aaaatggcga tccccgagca accgctggag atcctgcgta ctctgcacag ctttgacccg
    31081 tgcctcgcct gttcaacaca cgtgctgggc gacgacggta gcgagctgat ctccgtgcag
    31141 gtgcgttaac agcgaaggag aatcatcatg caacagaaaa gcgacaacgt tgtcagccac
    31201 tatgtctttg aagcgccagt ccgcatctgg cactggttga cggtgttatg catggcggtg
    31261 ttgatggtca ccggatactt tatcggcaag ccgctacctt ccgtcagcgg cgaggcgacg
    31321 tatctgttct atatgggcta catcaggtta attcacttca gcgccgggat ggtttttacc
    31381 gtggttttgc tgatgcggat ctactgggct tttgttggca atcgatactc ccgcgagctg
    31441 tttatcgtgc cggtatggcg taaaagctgg tggcagggcg tgtggtatga aatccgctgg
    31501 tatctgtttc tggcaaaacg tccgagtgcc gatataggcc ataatcccat cgcccaggcg
    31561 gcgatgttcg gctatttcct gatgtcggtc tttatgatca tcactggttt tgcgctgtac
    31621 agcgaacaca gccagtacgc tatttttgcg ccgttccgtt atgtggtgga atttttctac
    31681 tggacgggtg gcaactcaat ggacattcac agctggcatc ggctggggat gtggctgatt
    31741 ggcgcgtttg tgatcggtca tgtctacatg gcgctgcgtg aagacatcat gtccgacggc
    31801 acggtgatct ccaccatggt caacggctac cgtagccaca aatttggcaa aataagtaac
    31861 aaggagtgtt catgagcgag caacgcgtgg tggtcatggg gctgggcaac ctgctgtggg
    31921 ccgatgaagg cttcggcgtg cgggtggcgg aacggctgta tgcccattac cactggcccg
    31981 agtatgtgga gattgtcgat ggcggtactc agggactgaa cttgctgggg tatgtcgaaa
    32041 gcgccagcca tctgttgatt ctcgatgcca ttgactacgg gctggaacct ggaacgctgc
    32101 gaacctatgc cggagaacgc attccggctt atctcagcgc gaagaaaatg agcctgcatc
    32161 agaacagttt ctccgaagtg ctggcgctgg cagatatccg cggacatctg ccagcacata
    32221 ttgccctcgt cgggctgcaa cccgcgatgc tcgacgacta cggcggcagc ctgagcgaac
    32281 tggcacggga gcaactgctc gctgcggaac aatcggcgct ggcgcagttg gctgcgtggg
    32341 gaagtgtgcc gcaactggct aatgaatcgc gttgtctcaa ttatgactgt ctgtcgatgg
    32401 aaaattacga aggcgttcgc ttgcgccagt accggatgac acaggaggag cagggatgag
    32461 caacgacacg ccatttgatg cgttgtggca acgaatgctg gcgcgcggct ggacgccggt
    32521 cagtgaatcc cgtcttgacg actggcttac gcaagcgcca gacggcgtgg tgttattaag
    32581 cagtgacccg aaacgcacgc cagaggtcag cgataatccg gtaatgattg gcgaattact
    32641 gcacgagttt cccgactata catggcaggt ggcgattgct gaccttgagc agagcgaagc
    32701 catcggcgat cgctttggcg tctttcgctt tcctaccact ttagtgttta ccggcggaaa
    32761 ctatcgcggc gtgctgaatg gtattcaccc gtgggcggaa ctgataaacc tgatgcgcgg
    32821 gcttgtcgaa ccgcagcagg agcgtgcctc atgagcgaaa cttttttcca tctgctgggg
    32881 ccaggaacgc aaccgaacga tgacagtttc agcatgaatc cactgccgat cacctgtcag
    32941 gtgaatgatg aaccgagtat ggcggccctg gagcaatgtg ctcacagccc gcaggtgatt
    33001 gcgctgttaa acgagttaca acatcaacta agcgaacgcc agccgccgtt gggcgaggtg
    33061 ctggcagtcg atctgttaaa tctcaacgcc gacgatcgtc actttatcaa tacgcttctc
    33121 ggggaagggg aagtgtcagt gcgcattcag caggctgacg acagtaaaag tgaaatacag
    33181 gaggcgatct tctgcggatt atggcgggtg cgcagacgtc gcggcgaaaa gttgctggag
    33241 gacaaactgg aggctggctg cgcgccgctg gcgttgtggc aggcggcaac gcaaaatctc
    33301 ttgccgacag attcgctgtt accgccgccc attgatggcc tgatgaatgg cctaccgttg
    33361 gcgcatgagt tactggcaca tgtacgtaac cccgacgcgc agccgcacag cattaatctg
    33421 acgcaattac ccatcagcga ggctgatcgg ctttttctct cacgtctctg tgggccggga
    33481 aatattcaga ttcgtaccat tggctatggc gagagctata tcaacgccac ggggttacgc
    33541 catgtctggc atttacgctg tacggacacc ttaaaaggcc cgttactgga aagttatgaa
    33601 atctgcccaa taccggaagt ggtgctggca gcgccagaag atttggtcga ctctgcgcag
    33661 cggcttagcg aggtatgtca gtggctggcg gaagctgcac cgacgtaaaa agacggtaag
    33721 tatcgctttc agtcttatga atatcgcaat cggcgaatac ctctggtcgt agagtttcag
    33781 gataaagagg gagatctaac attatcgggt tatttttctc tcttcgccta caggagtgcg
    33841 catgtgggat gtcattgatt tatcgcgctg gcagtttgct ctgaccgcgc tgtatcactt
    33901 tttatttgta ccccttaccc tggggctgat ttttttgctg gctatcatgg aaaccattta
    33961 cgtggtcacc ggcaaaacaa tctaccgcga tatgacgcgc ttctggggta agctcttcgg
    34021 tatcaatttt gctcttggcg tggctaccgg cctgaccatg gagtttcagt ttggtactaa
    34081 ctgggcattc tattccaact atgtgggcga tatttttggc gcaccgctgg cgatggaagc
    34141 attaatggcc ttcttcctcg aatccacctt tgtcgggctt ttcttcttcg gctggcagcg
    34201 gctgaataaa taccagcacc tgctggtgac gtggctggtg gcgttcggtt caaatctctc
    34261 tgcattgtgg atattgaatg ccaacggttg gatgcaatac ccgaccggtg cgcattttga
    34321 tatcgacacc ctacgtatgg agatgaccag cttcagcgag ctggtcttta acccggtcag
    34381 ccaggtgaaa tttgtgcaca ccgtaatggc gggctacgtg accggggcca tgtttattat
    34441 ggcgatcagc gcctggtatt tactgcgcgg acgggagcgc gatgttgcat tacgctcgtt
    34501 tgccatcggt tccgtcttcg gtactctggc gattatcggc acgctacaac tcggggacag
    34561 ttctgcgtat gaagtcgcgc aagtacaacc ggtaaaactg gcggcgatgg aaggggagtg
    34621 gcaaacggaa cctgctcctg caccgttcca tgtggttgcc tggccggaac aggatcaaga
    34681 gcgtaacgcc tttgccatca aaattcccgc gctgttaggg atcctcgcca ctcactcatt
    34741 agataaaccc gtgccgggtc tgaagaattt gatggctgaa acctacccac gcttgcaacg
    34801 cggacgtatg gcctggctgt taatgcagga aatatcgcaa ggcaatcgtg agccgcatgt
    34861 gttgcaggca ttccgggaac tggaaggtga cctgggctac ggcatgttgc tctcccgcta
    34921 tgcgccggat atgaatcatg tcacagccgc acagtaccag gcggcgatgc gtggcgcgat
    34981 acctcaggtt gcgccggtat tctggagttt ccgcatcatg gtgggctgtg gttccctgct
    35041 gctactggtg atgctgattg cgcttgtcca gacgctgcgt ggcaaaatcg accagcatcg
    35101 ctgggtgctg aaaatggcgc tctggagttt gccgttgccg tggattgcga ttgaagccgg
    35161 gtggtttatg accgagtttg gtcgtcagcc gtgggcgata caggacatct taccgacata
    35221 ctccgcgcac tccgctttaa ccacaggaca actggctttc tcactgatca tgatcgtagg
    35281 gctttacacc ctgttcttaa tcgccgaagt ctacctgatg cagaaatatg cccgtctggg
    35341 gccgagcgcg atgcagagtg aacaaccgac gcagcaacag gggtaaagga gaaaatcatg
    35401 tttgattatg aaacattgcg cttcatctgg tggctgctga ttggcgtgat cctggtggtc
    35461 tttatgatct ccgacggatt tgacatgggg atcggctgtc tgctgccgct ggtggcgcgt
    35521 aatgatgatg aacgccggat agtgataaac agcgttggtg cacactggga aggcaaccag
    35581 gtctggttga tcctcgctgg tggggcatta tttgccgcct ggcccagagt gtatgcagcg
    35641 gcgttttccg gcttttatgt ggcgatgatc ctggtgctgt gctcactgtt cttccgcccg
    35701 ctggcctttg attatcgcgg aaaaatcgcc gatgtacgct ggcgtaaaat gtgggacgcc
    35761 ggtctggtca tcggcagtct ggtgccgccg gtagtcttcg gtatcgcctt cggcaacttg
    35821 ttgctcggcg tgccgtttgc cttcacaccg caattacgcg tggagtatct cggcagcttc
    35881 tggcaactgc tgacgccatt ccctttattg tgcggattgc tcagccttgg gatggtaatt
    35941 ttgcaaggtg gcgtctggtt acaactgaaa actgttggtg tgattcatct gagttcacag
    36001 ctggccacca aacgcgctgc actgttggtg atgctgtgct ttttgctggc gggttactgg
    36061 ctgtgggtcg gtattgatgg ctttgtactg ctcgcccagg atgctaacgg tccttccaat
    36121 ccgttaatga aactggtggc agtgctacct ggtgcctgga tgaataattt cgtcgaatcg
    36181 cccgttttgt ggatcttccc gctgctgggg ttcttctgcc cattgctgac ggtgatggcg
    36241 atttatcgtg gtcgcccagg ttggggattt ttaatggcgt cattgatgca atttggcgtg
    36301 attttcacgg caggcatcac gctgttcccc ttcgtcatgc cgtcaagcgt gagtccgatc
    36361 tccagcctga cgttgtggga cagcacttcc agccagctga cgctgagcat tatgttggta
    36421 atcgtgctga tatttttgcc cattgtgttg ctctacactc tctggagcta ctacaaaatg
    36481 tgggggcgca tgacaacaga aactctccgc cgtaacgaaa acgagttgta ctaaggagca
    36541 ggaacaatgt ggtatttact ttggttcgtc ggcattttgt tgatgtgttc gctctccacc
    36601 cttgtgttgg tatggctgga cccgcgtctg aaaagttaac gaacgtaggc ctgatgcggc
    36661 gcattagcat cgcatcaggc aatcaataat gtcagatatg aaaagcggaa acatatcgat
    36721 gaaagcgatc ttaatcccat ttttatctct tctgattccg ttaacctcgc aatctgcatt
    36781 cgctcagagt gagccggagc tgaagctgga aagtgtggtg attgtcagtc gtcatggtgt
    36841 gcgtgctcca accaaggcca cgcaactgat gcaggatgtc accccagacg catggccaac
    36901 ctggccggta aaactgggtt ggctgacacc gcgcggtggt gagctaatcg cctatttcgg
    36961 acattaccaa cgccagcgtc tggtggccga cggattgctg gcaaaaaagg gctgtccgca
    37021 gcctggtcag gtcgcgatta ttgctgatgt cgacgagcgt acccgtaaaa caggcgaagc
    37081 cttcgccgcc gggctggcac ctgactgtgc aataaccgta catacccagg cagatacgtc
    37141 cagtcccgat ccgttattta atcctctaaa aactggcgtt tgccaactgg ataatgtgaa
    37201 cgtgactgac gcgatcctca gcagggcagg agggtcaatt gctgacttta ccgggcatcg
    37261 gcaaacggcg tttcgcgaac tggaacgggt gcttaatttt ccgcaatcaa acttgtgcct
    37321 taaacgtgag aaacaggacg aaagctgttc attaacgcag gcattaccat cggaactcaa
    37381 ggtgagcgcc gacaatgtct cattaaccgg tgcggtaagc ctcgcatcaa tgctgacgga
    37441 gatatttctc ctgcaacaag cacagggaat gccagagccg gggtggggaa ggatcaccga
    37501 ttcacaccag tggaacacct tgctaagttt gcataacgcg caattttatt tgctacaacg
    37561 cacgccagag gttgcccgca gccgcgccac cccgttattg gatttgatca tggcagcgtt
    37621 gacgccccat ccaccgcaaa aacaggcgta tggtgtgaca ttacccactt cagtactgtt
    37681 tattgccgga cacgatacta atctggcaaa tctcggcggc gcactggagc tcaactggac
    37741 gcttcccggt cagccggata acacgccgcc aggtggtgaa ctggtgtttg aacgctggcg
    37801 tcggctaagc gataacagcc agtggattca ggtttcgctg gtcttccaga ctttacagca
    37861 gatgcgtgat aaaacgccgc tgtcattaaa tacgccgccc ggagaggtga aactgaccct
    37921 ggcaggatgt gaagagcgaa atgcgcaggg catgtgttcg ttggcaggtt ttacgcaaat
    37981 cgtgaatgaa gcacgcatac cggcgtgcag tttgtaatgc ataaaaaaga gcattcagtt
    38041 acctgaatgc tctgaggctg atgacaaacg aagaactgtc taatgcgtag accggaaaag
    38101 gcgttcacgc cgcatccggc cactttcagt tttactcttt ctcggagtaa ctataaccgt
    38161 aatagttata gccgtaactg taagcggtgc tggcgcgttt aatcacacca ttgaggatag
    38221 cgcctttaat attgacgcct gcctgttcca gacgctgcat tgacaaactc acctctttgg
    38281 cggtgttcaa gccaaaacgc gcaaccagca ggctggtgcc aacagaacgc cccacgaccg
    38341 cggcatcact caccgccagc atcggcggcg tatcgacaat caccagatcg taatggtcgt
    38401 tcgcccattc cagtaattga cgcatccgat cgcgcatcag cagttcagac gggttaggtg
    38461 gcacctgacc gcgagtaatc acatcaaagc ctcctttgcc aaaatgctgg atcactttgt
    38521 tgagctcatc tttacctgcc agatattccg acaagccatg ttcattactc acggtaaaca
    38581 ggttatgcga ataaccacgg cgtaagtcgg catcaataaa taacactttt tgatcggact
    38641 gggcgatcac cgctgccaga gttgaactga caaacgtttt accactgtct ggcgtcgcac
    38701 cggtgatcat cagaatgtta ttctccgtct ccatcatagc gaagtgcaga ctggttcgta
    38761 gcgcacgtac ggcttccaca gcagaatccg ccgggttatc caccgccagg aaggggatat
    38821 ttttagtacg atggcgctgc tgattagaaa ataaattttt cttacgcaga cgggtacgtt
    38881 tatccagcca ctcggacatt gggatagtgg cataaacgct gatgccgtgc tcttccagtt
    38941 gttccggggc ttctacacca cgacgcaaca tcgcacgcgc cagcacggca cccaccgaaa
    39001 taaacaggcc aagaataaaa ccaagcacca cgttcaacgc ttttttcggt ttcactggct
    39061 gcggctgagt gactgctggg tcgataatcc gcacgttacc aatggcactg gatttcgaaa
    39121 tactcaactc ctgctggcgg ttaagtaatt gcagatatac cgcacggccc gcttctacgt
    39181 cacggcttaa acgtaacact tcctgttgag tggaaggcat tgccgatacc cgcttattca
    39241 ggcgtttgcg ttcttgctcc agcgtctggc gtttttccag cagcgcacga taagttgggt
    39301 gatctttctt atacagctgg gagatctctg cctcgcggaa agtcagctca ttgagttgat
    39361 tatcaacgtt cacaatctgc tcaagaacgg ctttggcttc taggttaagg tcaaccgaat
    39421 cgcgctgctg gcgataaacg ttgagttttt cttccgcctg gtccagctcg ctgcgcactt
    39481 caggcaactg gcgctgtaag aattcaaggc tttgtgaatc ctgcgccgcc tggcgagcga
    39541 tattctgttg caaatagttg ttagcgatgc tgttcagaat acgagtaatc aactggggat
    39601 catcaccagt catggtaagt tccagcatcc cgctttcttt actgcgttcg ctaacggtaa
    39661 aggtttcctg caatgcgtta atcgcttcca gttcggtacg ctggctcagg acaaactgtg
    39721 ttcctggttt ggccttaatg tccgcgatag tcagcgcaac gccatctttt tccagacgct
    39781 ggccgaccat accattgacg gtgaactctt caccttccag tgtatagtgg ccgttttccc
    39841 caaccgtgag tgtcagttgc tgatcctgac cattcagttg tggaatatgc atccagctga
    39901 tcgccagctc acctggtttt tctttggtta atctcgccca gccgcgaccc acaatcggaa
    39961 aatacttctg ctcaactatg tcgtgcagat tcagttcagc aatggtttta ccgagaatca
    40021 tgcgcgattg cagcagttgg atctccggtg cagactcggg cgatgagtta gggatcatat
    40081 cgctcaggcc gctgagaatg gcgttgccct gtttttgctc aacctggacc agagtatctg
    40141 cctgataaat tggtgtgctt aacagcgagt aagcgacagc gatcagcgtg aataacgcgg
    40201 ttacgctgat aataaactta cggtgatccc ataactcgcc gaccagacga agcagatcga
    40261 tctcattttc ctgagtgctg cctggtggcg tattcatatt tttagttgtc atcgtatttt
    40321 ataaactcaa attaccggct gaggcgcttc gcccattcct gactggcgcg ctccaacata
    40381 ccgtagacat gttcaaatgc gtcctgactt ttacgatagg gatccgggat ctctttctgt
    40441 tccagccatt gcccaaacag cattgtcttc ccgcggacct cgggagcgat tgcggtaacc
    40501 tgagcaatat gctctgactc cattgccaga atcagatcgt aatttcgtgc catctcagca
    40561 gtgagcttac gtccggcatg tccttcaaga gatacgccat gattagcggc aacatctgct
    40621 gctgtcgcat ctgcagggtg ttttaccaga ccatgaacac ctgccgattt gacttttacg
    40681 cctggcagac gtttacgcag caagcgctcg ccaatgggcg aacggcaaat gttgccggta
    40741 caaaccacca ggattgagtt aaattttagt tgggccatgt ttgaatccac cgtgaagtct
    40801 ctgtcaggtc atgcaccccg gaaatcgtcg gtaccagcag agaaatcaca cggttccaac
    40861 gagccagcgg cgcggtagtg acataaacaa tgtcgtaagg ttcaagctgg aattccgttc
    40921 cgagtatcat tgccgatgcg tccttcgcat tcagctgata aatattggcg attttgccat
    40981 tctgcttgtt ttgcgttgca cgaataacaa aaatccccgt cgcatcagcg acatcctgat
    41041 tcataccctc agcgttaccc agcgcttccg ccagggtcat accgctgcgg tccattttca
    41101 gcgtgctctg tttacccact tcacccatca cgaagacttt cagatcatcg ttgcgcggaa
    41161 taaataaaat atcgcccggg tgcagcagtt tgttctggcg caagtcgcca cgctgcatca
    41221 gcgcatagag gttcactttg gtctttacgc cattctgggt gagcaccacg ttgcgccagt
    41281 ctgcatctgc ggtcagaccg cccgcggcgt taatcgcatc catgatggtc agaggaatat
    41341 tggtaatcgg ctgctgaccc gatttgctga cttcgccggt gacgtacgct ttttgcgatc
    41401 ggaacgccgc gacgctgaca tcgacttgcg ggctttcaat gactgaatcg agacgcgctg
    41461 taatctcgtt gcgtacctgg gtaagtgttt tcccggccac tttcaggcga ccaatatagg
    41521 ggtagaaaat cgcaccatcg gcgttaaccc agttgccggt atcactggcg ctacggtatt
    41581 gccctgcggg ggtggttagt tccgggtgat cccagaccgt aaccatcaac acatcaccaa
    41641 tcccgatacg atattcatag tttgcgattt gctgctccag ctccgggtta gcctgcgaca
    41701 taatcggttt ggcgcgcaac tgatcaatca atcctggcgt gacgggatag atattgacca
    41761 ttttgtccag gtcatactga ttgtcaggta gttcaatgac atccttgttc ctggtcgata
    41821 aattctgacc aggaacaagg gtacatgctg tcaaagacag aacagccaat gtcagtaccg
    41881 aaaacttaaa aatgttcttt ttcattggtt tatatttatc agtcaatgta atgttatgaa
    41941 ttacgtcagg ttattgtcca accggtactt ctcgctctgc cgtcattgga tagagctggt
    42001 atttgcgtga cagcatttgt ccaccatcac gcgtcaatgg tgtccacgaa accaccgcgc
    42061 ggttgcggtt aggtccaatg gtcatcaagt cgaatggaat cgagatataa aaacctttac
    42121 taaagccgcc ttcgccgtag tcatctttag atacgttact gattgccgcc cataccccta
    42181 ccgccacgcc gctgtcaaag cgtttggcga cgtcgatcgt tgctccttta tcttttgcca
    42241 gatattgccc aacgctaagt ttcatcagta cgccattgag cgtcggcggg ttccagtaag
    42301 ccgtcacgaa accagttggc gtggaataat cggtgaagcg catcatgttg tcccagtcac
    42361 gttgcttcac gtagttaacg tccacaccca gcgcccagct ggcatctagc gggcgataaa
    42421 gcagctcgga accgacaccg gcgtacatcg tttccagata accgccatac acctggccat
    42481 agaaaccatt gcctaagtcg gcaaagtagt tcgcctgcaa gttgttgaga taaacgtcat
    42541 tgcgaacgta atcacggata tgcgtgcgca cgcggggcag ggtagagtcc gcgggcaaca
    42601 gcgaagactt aaacttgtcg tagttgttgt aaatattggt gaaaataccg ccatcaagca
    42661 gcaggtggtc ggtaaaccag tagcgggcac tggacatcag ccccagctgg aacatataga
    42721 aatcttccgg gccgcccagc gactgtgaaa gtgttgggtt gaaagagtag ctaaagcgat
    42781 cttcacgaat acggtagccc cgaccaaagg cagaaagatc ttctgcttca acacgttgtt
    42841 gttgaagttg ctctgattga ccaggcgctg taccagccag ctgtttgcgc aggctggcta
    42901 cgtcggtttc ggtagtcacc atcgccatat gctcgcgctt ttgcgtcacg ctaatcttct
    42961 caacgccttg cggcaggtta ttcaccagaa tccggttggc acgatcgacc gcttcgcgag
    43021 aatcacggta tttgtattgt tgaccagaca tatacagcgt cttatcgcgc agctgaattt
    43081 ccggtgcttc aaaacctgcg ttgtacttca gcgcggtaag ttggttcgct actgtggtgt
    43141 actgcaatcc ttcagattca ggcgcaggtt gatatgccgg ttttggcgta tcgcgcaggg
    43201 cagggcgcag atcgttgaaa ttggtccgta acgtgaagcc aaacatcaac gtgttaccgc
    43261 gttcataact caggttgaga tctgcccagc tggcagcgcg ataaactgcg ccgacgttga
    43321 aatggcttgc ctgaggcagt ttgccagcga aatcattctg gtaattgttg ccatcgtatt
    43381 cgagtttcag acgcagggga ttccacggcg tttgatactc aatgccgcca aagatggaag
    43441 ccgggccacg aaagatatcg ctaaagctga tatcgcccgc atcgtgagac tctgcgcgat
    43501 gacaatattt atcgttcacc cggcaaaacg ggttggtaat attgcccgca ttaccggcgt
    43561 agccccatgc catcccgagg gtgaaatcaa atggccccgc ttgcttgctg gccaccagat
    43621 actcaccgtc aaacagaccc gtaccagcaa tatcacgttt accaaacgcc acttgcggta
    43681 gccagtaacc ttcttcccac aggcgaagtt taaaatcgaa tgatttgtct ttgtatgact
    43741 gatcgccgct gaaatcttcc cactggctat atttgcgtgt gcgcacatcc gtataacgaa
    43801 tggtgccttc cagccatggg aacagcgcca cggaggtgga gtagaaccgg tattgatcgt
    43861 tatcccggta gttgacgctg aattcacctt ccggtgcgat gcgcgcattt ggcatctgca
    43921 acaatcctgt gccgccaaag tctgattgcg acgaacccag cggatccggg taggttaata
    43981 cttcagcatg acaggcactg gagacggcaa tggccaggca gcttaaaaga taagaattct
    44041 tcttcatcag tcaggaacac gttgcgttaa aacagacaca atctggtcat ttaaatcagc
    44101 atatttttcg ggcagtacat gcgcagaaaa gcccaaccaa agctggctgc caggcggtgg
    44161 ttccacatgt cttttattcc acagggcaac aggggcgacg acagtctcac cctcaggcgt
    44221 aatcaccata acgttattct tgtccgcgcc tgcaaggcgg gggtgatctt gcagataatc
    44281 ggtaacgcta cggcctgcct gccagggcaa ttgcccggcc ccagaaaccg cacccagcag
    44341 agtaatagtc accggacgct gtacggtata gagcgtatag tccccgacca acgggggatt
    44401 gctgttctca tccactcgaa caaaatcagg gtccagtttg accggtaagc gaccagtgat
    44461 attgagattg agtaactgct ggcgcacaga tttaatcgtt gccgccacat cgtcgtcagc
    44521 ttctgcttcc caggaagcaa gctgtgccat gacatgttgg tagtctttaa gcgctttagc
    44581 tttcgccgca gagtcggtca gcaaggcacc aggccaccag aggcgatcgc gcagttgcgg
    44641 ttgtgtcacc aactggacaa cattttccat ggggccaacg gaaagtgtct gttgttcgcc
    44701 aggcagataa atagtcaccg ttccttgcgc aaaggcatgg ggtgtcatta cgtaaagtac
    44761 gctggcaatg aaatacgact gtaatttatt catggtgcgg gtttaagaaa cgtcatttct
    44821 acgggaataa cccctgcgcc taacatttgc cgactttgac gaacctggcc agtagccgaa
    44881 tcgacccaga atgtgttttg ccactgtttt tctggccgcg tggaagtcac ctcttcctgc
    44941 cagacattac acagcacatt gcgacccgca atattcagcg tctctaatcc agcaaaggaa
    45001 aaagtagaac tcagggtcgc agagcgaaaa tggttgtctt cactccagag aatatcgcgc
    45061 gtccagcggc tgccatcttt tattgccagc gcgttacgca gagggtcctg cccggaattt
    45121 gtcacttcca gcagattatt attaagtttg acggttttta aaagacgtcc attgtgggta
    45181 accagcatgg cgttatcctg ggataaccat ttgctttgtt cttgttctat atatcccagt
    45241 accacaaaga ttcgttgccc ttcattaagg cgtaaataca tcgtggaata aggcaacgcc
    45301 tggatctgct gatccgctac ggtgatatcc tgattatcga aaaggctggc gcgaaatgta
    45361 tcgaccatac tttgctggct gtgcgtacat cccgcaagaa atagtgcgaa aatcgataaa
    45421 ataagagggc gcacctttaa tccttgaaag gttaattaaa aaaatgcaaa tctttaaagc
    45481 ccggtacgaa tatcgcaacc gggtatcatt taactttaat ttcgacgcgt taaagcagta
    45541 ttagcgtgta gtggacgtgg tggttgaggt gttatgagaa ccgccatcgc cactgtcatt
    45601 tgctgcaaca actgccaacg tcgccacacc aacagccgtt gccacaccga caactgtcgc
    45661 cgtgccgaca gcgccagcag ccacgccacc agtggtagta gcagtcgtgg ttgcgccggt
    45721 agtggtcgtt gtggtgccag tagtacccgt cgttgcctcg gtcccgtttg ttgcttcagg
    45781 ggcagcaaac gcagctggcg tcgtcaacat gaaggccata aggattgcag aaagcttgtg
    45841 tttcataact tttcctttat tcatcgcatg gacaatacgt acgcttgaga tcaggattgt
    45901 ctactttgag tataacatcc ttgtgaaatc tccctttcct cataccgctt gcactatata
    45961 ctgcgggtag gaaaggcgcg cagagggaaa tataagattg ttaactatcc ccctctggaa
    46021 atacgacttg tgagcataag ataaatctta gcaaaataca aattaaagat aaggaaattg
    46081 ttacgaaagc tattaattat tgctggtaat atagtttcaa gtggtactat aactggctgt
    46141 taaatggcat ttataactat taggtgcaga gatattcgct taaatggatt agtttctttg
    46201 tggaaaagta actgatgtta taatatatga tgttgactat tgcactattc caataaaacc
    46261 agtcagcttt aaacaagcag cgtcatatta agagagataa acatttgccg ctgttggtcc
    46321 tcgcaggcca tttacgcggc aaaattccac acgtaatcct ggtataagca cttctgcgtc
    46381 gcggggagtg aatgcggaaa tatggacctg aacttcttta cgaccgtcgg aggggataat
    46441 gaatcctttg ccgcttttgc gatcaaaggt tttgacaatt cctgtcattt tacgggacaa
    46501 acaaattcct tactgaaaat actgcgctgc actatacggg gttaataaaa taaagccagc
    46561 gatatttaag accgccggac ggctaaaata aaatttgctt aatctcaatt atcatgcgtt
    46621 aatagctgcg tcggtttgaa agacagacag catacaaagt agtttactaa agcagttctc
    46681 attatcaggc attatcccct tcttttgagt ctctctcctg aacactaagt agtttctgta
    46741 ttaaagccct gtttgccgaa aggcccaaaa tgaaggaagt aaaatatgtc taataaaatg
    46801 actggtttag taaaatggtt taacgcagat aaaggttttg gctttatcac tcctgatgat
    46861 ggcagcaaag acgttttcgt ccatttcact gccatccaga gcaatgaatt ccgcacgctg
    46921 aacgaaaatc agaaagttga attttctatt gagcaggggc aacgtggccc cgcggcagcg
    46981 aacgttgtta cgctctaagg ttgccgttat tactcaacat ctccatttcc gctgtccatg
    47041 ttgtcatggt tcacagtacc gcacatcggc attcgatgtg acggagcgaa atcctttggg
    47101 cgctaagtgt attttttgta aatcgacgat gatcaccttt gataacgtca cgctgcaaat
    47161 acgcactgac catgcgccgc tggatttcac aaaataatat caggctccct cgt
//
LOCUS       DAJTNN010000008        43881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTNN010000008 DAJTNN010000000
VERSION     DAJTNN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.008"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            85..609
                     /gene="lpfE"
                     /locus_tag="OO150_000451"
     CDS             85..609
                     /gene="lpfE"
                     /locus_tag="OO150_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HCR5514711.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            862..2553
                     /gene="eptB"
                     /locus_tag="OO150_000452"
     CDS             862..2553
                     /gene="eptB"
                     /locus_tag="OO150_000452"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5514712.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            2645..2721
                     /locus_tag="OO150_000453"
     tRNA            2645..2721
                     /locus_tag="OO150_000453"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2679..2681,aa:Pro,seq:cgg)
     gene            3465..5072
                     /gene="dppA"
                     /locus_tag="OO150_000454"
                     /pseudo
     CDS             3465..5072
                     /gene="dppA"
                     /locus_tag="OO150_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
     gene            5380..6399
                     /gene="dppB"
                     /locus_tag="OO150_000455"
     CDS             5380..6399
                     /gene="dppB"
                     /locus_tag="OO150_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5514713.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPASGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            6409..7311
                     /gene="dppC"
                     /locus_tag="OO150_000456"
     CDS             6409..7311
                     /gene="dppC"
                     /locus_tag="OO150_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5514714.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            7322..8305
                     /gene="dppD"
                     /locus_tag="OO150_000457"
     CDS             7322..8305
                     /gene="dppD"
                     /locus_tag="OO150_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5514715.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            8302..9306
                     /gene="dppF"
                     /locus_tag="OO150_000458"
     CDS             8302..9306
                     /gene="dppF"
                     /locus_tag="OO150_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5514716.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(9336..10607)
                     /locus_tag="OO150_000459"
     CDS             complement(9336..10607)
                     /locus_tag="OO150_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5514717.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            11083..11190
                     /locus_tag="OO150_000460"
     CDS             11083..11190
                     /locus_tag="OO150_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5514718.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(11277..12956)
                     /gene="bcsG"
                     /locus_tag="OO150_000461"
     CDS             complement(11277..12956)
                     /gene="bcsG"
                     /locus_tag="OO150_000461"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5514719.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVETTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTTVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(12953..13144)
                     /gene="bcsF"
                     /locus_tag="OO150_000462"
     CDS             complement(12953..13144)
                     /gene="bcsF"
                     /locus_tag="OO150_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5514720.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(13141..14712)
                     /gene="bcsE"
                     /locus_tag="OO150_000463"
     CDS             complement(13141..14712)
                     /gene="bcsE"
                     /locus_tag="OO150_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5514721.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLTHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14985..15173
                     /locus_tag="OO150_000464"
     CDS             14985..15173
                     /locus_tag="OO150_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5514722.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            15185..15937
                     /gene="bcsQ"
                     /locus_tag="OO150_000465"
     CDS             15185..15937
                     /gene="bcsQ"
                     /locus_tag="OO150_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5514723.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHRQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            15934..18552
                     /gene="bcsA"
                     /locus_tag="OO150_000466"
     CDS             15934..18552
                     /gene="bcsA"
                     /locus_tag="OO150_000466"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR5514724.1"
                     /translation="MSILTRWLLIPPVNARLIGGYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRP
                     ERSETAQPSDQALAQQ"
     gene            18563..20901
                     /gene="bcsB"
                     /locus_tag="OO150_000467"
                     /pseudo
     CDS             18563..20901
                     /gene="bcsB"
                     /locus_tag="OO150_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            20908..22014
                     /gene="bcsZ"
                     /locus_tag="OO150_000468"
     CDS             20908..22014
                     /gene="bcsZ"
                     /locus_tag="OO150_000468"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5514725.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            21996..25469
                     /gene="bcsC"
                     /locus_tag="OO150_000469"
                     /pseudo
     CDS             21996..25469
                     /gene="bcsC"
                     /locus_tag="OO150_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            25551..27539
                     /gene="hmsP"
                     /locus_tag="OO150_000470"
     CDS             25551..27539
                     /gene="hmsP"
                     /locus_tag="OO150_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5514726.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            27722..29008
                     /gene="dctA"
                     /locus_tag="OO150_000471"
     CDS             27722..29008
                     /gene="dctA"
                     /locus_tag="OO150_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5514727.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPVSVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            29228..30724
                     /locus_tag="OO150_000472"
     CDS             29228..30724
                     /locus_tag="OO150_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5514728.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(30820..31749)
                     /gene="kdgK"
                     /locus_tag="OO150_000473"
     CDS             complement(30820..31749)
                     /gene="kdgK"
                     /locus_tag="OO150_000473"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5514729.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSTAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            31981..32748
                     /gene="pdeH"
                     /locus_tag="OO150_000474"
     CDS             31981..32748
                     /gene="pdeH"
                     /locus_tag="OO150_000474"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5514730.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            32818..34878
                     /locus_tag="OO150_000475"
     CDS             32818..34878
                     /locus_tag="OO150_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5514731.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEQLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(35112..36434)
                     /locus_tag="OO150_000476"
     CDS             complement(35112..36434)
                     /locus_tag="OO150_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5514732.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLLILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETQHQSL"
     gene            complement(36845..37858)
                     /gene="yhjD"
                     /locus_tag="OO150_000477"
     CDS             complement(36845..37858)
                     /gene="yhjD"
                     /locus_tag="OO150_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5514733.1"
                     /translation="MTQENEIKRPTQDLELEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMLGK
                     TQP"
     gene            complement(37907..38806)
                     /locus_tag="OO150_000478"
     CDS             complement(37907..38806)
                     /locus_tag="OO150_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5514734.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKNLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            39326..39928
                     /locus_tag="OO150_000479"
     CDS             39326..39928
                     /locus_tag="OO150_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5514735.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(39979..41628)
                     /locus_tag="OO150_000480"
     CDS             complement(39979..41628)
                     /locus_tag="OO150_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5514736.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVKRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            42032..43429
                     /gene="ccp"
                     /locus_tag="OO150_000481"
     CDS             42032..43429
                     /gene="ccp"
                     /locus_tag="OO150_000481"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5514737.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            43640..>43881
                     /locus_tag="OO150_000482"
     CDS             43640..>43881
                     /locus_tag="OO150_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5514738.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT        10539 a        11003 c        11926 g        10413 t
ORIGIN      
        1 tcccgttagc actaccggaa aattacccgc tgccggggat tatagcggta ttgctaccat
       61 acatgtcgaa ctggagtaac agtcatgaag cgaacaatac ttgcgttatg cctgctgcct
      121 ctttcctttt cgtcattcgc ggcaaccacg gatttagggc cgaaaggaac attgaaattc
      181 acccttaaaa tcagccaggg tgcttgcgaa ctggtgaaag acagcgttga ggttgacatg
      241 ggcaccgcgg ttctcaaaaa gccggtaaga atcggaacgg aaattaatcc cactcctttt
      301 tccatcggcc tgaaaaactg tagcgaagtg gtcagagcct acgtgactat ggacggtaca
      361 cccaatgcag acgactcaaa tctttttgcg ctggatgcgg gtggcgctac aggcgttggg
      421 ctgaaaatca aaaccgctgc tggagtacaa cagttcccgg aaaatacaac gccgacaccg
      481 gttgagtttg caattaacgg tgatggcgca catcaactga actatatcgc cagctacgtg
      541 cctattcgcg ccgatgcaac tattggtcgc gcagatgcaa cggtcgactt tactgtacag
      601 tacgaataat ttcacatctt aagggccagt taactggccc tttttactgc tttttgtaac
      661 gctaaagtct cttttcaaac ttgcattttt gtaaatttgt gcttcatgca cactctttcc
      721 ccacactttt tcgctttgct gtggtctact tattcgcgcg tgtagatttt acttatctga
      781 ctacctccgc acttttcccc tgccgggcct gaaaagccgc taagcagggt gttatcacct
      841 gtttgtccag ggtttgtttg catgagatac atcaaatcga ttacacagca gaagctgagc
      901 tttttgcttg caatctatat tggccttttt atgaatggcg cggtttttta tcgccgcttc
      961 ggcagctatg cgcacgattt taccgtctgg aaaggcgttt ctgctgttgt tgaactggcc
     1021 gccaccgtac tggtgacctt ctttttacta cgtcttctat cgctgtttgg ccgccgcagc
     1081 tggcgtattc tggcgtcgct ggtggtgctc ttttccgcag gtgccagcta ttacatgacc
     1141 ttccttaatg tggtcattgg ttatggcatc atcgcttccg tcatgaccac cgatatcgac
     1201 ctgtcaaaag aagttgttgg tctgaacttt attctctggt taatcgccgt tagtgcattg
     1261 cctcttatcc ttatctggaa taaccgctgt cgctacacct tgctccgaca actgcgaacc
     1321 ccagggcagc gtattcgcag cctggcggtc gtcgtactgg cgggtattat ggtttgggca
     1381 ccgattcgtt tgctggatat ccagcagaag aaagtggaga gggcgaccgg cgttgatttg
     1441 ccgagttatg gcggtgtcgt agcgaactct tatctgccat caaactggct ttctgcgttg
     1501 gggctgtatg cctgggcgcg ggtcgatgaa tcttccgata ataattcatt gcttaatccg
     1561 gcgaagaagt ttacatacca ggctccacaa aacgttgatg atacttatgt cgtgtttatc
     1621 atcggtgaaa ccacgcgttg ggaccatatg ggtattttcg gctatgagcg taataccacg
     1681 ccgaaactgg cccaggagaa aaatctggcg gcgttccgtg gttactcctg tgataccgca
     1741 accaaactct cactgcgttg catgtttgta cgtcaggggg gcgcggaaga taatccgcag
     1801 cgcacattaa aagaacagaa cattttcgcg gttctgaagc agttaggatt cagttctgac
     1861 ctctacgcta tgcagagcga aatgtggttc tacagcaaca cgatggcgga caacattgct
     1921 tatcgtgagc agattggtgc ggagccacgt aatcgtggca agccggtaga tgatatgttg
     1981 ctggtagacg aaatgcagca atcgctaggg cgcaacccgg atggtaagca tctgatcatt
     2041 ctgcatacca aaggttcgca ttttaactac acccagcgtt atccgcgtag cttcgcgcag
     2101 tggaagccgg aatgtattgg tgttgatagc ggctgtacca aagcgcagat gatcaactcc
     2161 tatgacaact cggtgaccta tgtggatcac tttatctcca gcgtgattga tcaggttcgc
     2221 gataagaaag cgattgtgtt ctacgcagct gaccacggtg agtcaattaa tgaacgcgag
     2281 cacctgcacg gcacgccgcg tgaattggca ccgccggagc agttccgcgt accgatgatg
     2341 gtctggatgt cagataaata tctggaaaat ccggccaatg cgcaggcatt tgcgcagctg
     2401 aaaaaagaag ccgacatgaa agtgccacgc cgtcacgtag agctgtacga taccatcatg
     2461 ggttgtcttg gctatacttc accggatggt ggaattaacg aaaacaacaa ctggtgtcac
     2521 atcccgcagg caaaagaggc agcggctaac taagcggcct gctgactttc tcgccgatca
     2581 aaaggcattt tgctattaag ggattgacga gggcgtatct gcgcagtaag atgcgccccg
     2641 cattcggtga ttggcgcagc ctggtagcgc acttcgttcg ggacgaaggg gtcggaggtt
     2701 cgaatcctct atcaccgacc aaattcgaaa agcctgctca gcgagcaggc ttttttgcat
     2761 ttgaagccca tgaggatgag aacctccggg ggctgggagg ttcgactcga cgtaggcctg
     2821 ataagacgcg ccagcgtcgc atcaggcaac ggcaccgcac ttattgtcgg atgcggcgcg
     2881 accgccttat tcgaccaaca caccacacat tttcctgcca actttgcgcc ccatatctca
     2941 ctatccatta acggatttgt gacagaaacc attgtctttt tttatatttg cttaaatctt
     3001 tttttgcgtt acttatggct ggcgctggca ttttccgctg tgcgttttag cgttcatggt
     3061 attaatcgct cagatatcca ccttttcctc agattttttt cattaaatgc ataaaactta
     3121 atcaccaaat gttgctaaat attaagctga ttgttctggt tatgttggag tagcacaaat
     3181 ttctctgctg caaatagctg tttgaagttt tttaatcttt gtttgtgggt tctcaccgtt
     3241 ggtgtaaatc ccggttggct gtattgacgt tttcacattc tgttgacaga ttgtaggtca
     3301 cgaggggcat tttatggagg atccgcactg ttacactgat gttaattagt acggcatccc
     3361 cacctcataa cgttgacccg accgggcaaa aaacaaaaaa ggtcaggtag cgacaaccca
     3421 ctgcaaaggg ttaaaacaac aaacatcaca attggagcag aataatgcgt atttccttga
     3481 aaaagtcagg gatgctgaag cttggtctca gcctggtggc tatgaccgtc gcagcaagtg
     3541 ttcaggctaa aactctggtt tattgctcag aaggctctcc ggaagggttt aacccgcagc
     3601 tgtttacctc cggcaccacc tatgacgcct cttccgtacc gctttataac cgcctggttg
     3661 aatttaaaat cggcaccact gaagtgatac cgggcctcgc tgaaaagtgg gaagtcagct
     3721 aagacggtaa aatctatacc ttccatctgc gtaaaggtgt gaagtggcac gacaataaag
     3781 aattcaaacc gacgcgtgaa ctgaacgccg atgacgtggt gttctcgttc gaccgtcaga
     3841 aaaacgcgca aaacccgtac cataaagtct ctggcggcag ctacgaatac ttcgaaggca
     3901 tgggcttgcc ggagctgatc agtgaagtga aaaaggtgga cgacaacaca gttcagttcg
     3961 tactgactcg cccggaagcg ccgttcctcg ctgatctggc aatggacttc gcctctattc
     4021 tgtcaaaaga atatgctgat gcgatgatga aagccggtac accggaaaaa ctggacctca
     4081 acccaatcgg aaccggtccg ttccagttac agcagtacca aaaagattcc cgtatccgct
     4141 acaaagcgtt tgatggctac tggggcacca aaccgcagat cgatacgctg gtcttctcta
     4201 ttacccctga tgcttccgtg cgttacgcga aattgcagaa gaatgaatgc caggtgatgc
     4261 cgtacccgaa cccggcagat atcgctcgca tgaagcagga taaatccatc aacctgatgg
     4321 aaatgccggg gctgaacgtc ggctacctct cgtataacgt gcagaaaaaa ccgctggatg
     4381 acgtgaaagt tcgccaggca ctgacctacg cggtgaacaa agacgcgatc atcaaagcgg
     4441 tttatcaggg cgcgggtgta tcagcgaaaa acctgatccc gccaaccatg tggggctata
     4501 acgacgacgt tcaggattac acctacgatc ctgaaaaagc gaaagccttg ctgaaagaag
     4561 cgggtctgga aaaaggtttc tccatcgacc tgtgggcgat gccggtacaa cgtccgtata
     4621 acccgaacgc tcgctgcatg gcggagatga ttcaggcaga ctgggcgaaa gtgggcgtgc
     4681 aggccaaaat cgtcacctac gaatggggtg agtacctcaa gcgtgcgaaa gatggcgaac
     4741 atcagacggt aatgatgggc tggactggcg ataacgggga cccggataac ttcttcgcca
     4801 ccctgttcag ctgcgccgcc tctgaacaag gttctaacta ctcaaaatgg tgctacaaac
     4861 cgtttgaaga tctgattcaa ccggcgcgtg ctaccgacga ccataacaaa cgcgttgaac
     4921 tgtacaagca atcgcaggtg gtgatgcacg atcaggctcc ggcactgatc atcgctcact
     4981 ccaccgtgtt tgaaccggta cgcaaagaag tcaaaggcta tgtggttgat ccattaggca
     5041 aacatcactt cgaaaatgtc tctatcgaat aattaaaagc catacaagac tgatggtaaa
     5101 ggcaaaactg cctgatgtgc tccgcttatc aggcctacga aaattctgca atgtattgaa
     5161 tttgcacgat tttgtaggcc ggataaggcg ttaacgccgc atccggcata aacaaagcgc
     5221 actttgtcaa caatctgtat acccggtggc gctgtgcctc tgcgtgcagc gccatccggc
     5281 agcaatactt cattctctgt ccgacaaggg cgggaattga tttgtgagca atacagacac
     5341 gcagttccag gctgcgggtc actatagaga atccgggtta tgttgcagtt tattctccga
     5401 cgtttgggac tcgtcatccc cacgtttatc ggtattaccc ttctcacatt tgcctttgtc
     5461 cacatgatcc cgggcgatcc ggtgatgatc atggcaggcg aacgtgggat ctcccctgag
     5521 cgtcacgcgc agctgctggc tgaactcggc ttagataaac cgatgtggca gcagtatctc
     5581 cattacattt ggggcgtaat gcacggcgat ctaggcattt caatgaaaag ccgaattcca
     5641 gtatgggaag agttcgtgcc gcgctttcag gccacgctgg aacttggcgt ctgcgcgatg
     5701 atttttgcta cggcagtcgg tattccggtt ggtgtgctgg ctgcggttaa acgcggttcc
     5761 attttcgatc acactgcagt tggcctggcg ctgaccggct actcgatgcc gatcttctgg
     5821 tggggcatga tgctgatcat gctggtttcg gtgcactgga acctgacgcc cgcctccggt
     5881 cgcgtgagcg atatggtgtt cctcgatgac tccaatccgt taaccggttt tatgctaatc
     5941 gacaccgcca tctggggtga agacggcaac tttatcgatg ccgtcgccca tatgatcttg
     6001 cctgccattg tgctgggtac tattccgctg gcggtcattg tgcgtatgac acgctcctcg
     6061 atgctggaag tgctgggcga ggattacatc cgcaccgcgc gcgccaaagg gctaacccgc
     6121 atgcgggtga ttatcgtcca tgcgctgcgt aacgcgatgc tgccggtggt gaccgttatc
     6181 ggcctgcagg tgggaacatt gctggcgggg gcgattctga ccgaaaccat cttctcgtgg
     6241 cccggtctgg gacgctggtt gattgacgca ctgcaacgcc gcgactatcc ggtagtgcag
     6301 ggcggcgtat tgctggtggc gacgatgatt atcctcgtca acttgctggt cgatctgctg
     6361 tacggcgtgg tgaacccgcg tattcgtcat aagaagtaag gggacatcat gtcacaggtt
     6421 actgaaaata aagtgattag cgcaccggtg ccgatgaccc cgttacagga gttctggcac
     6481 tattttaaac gcaacaaagg cgcggtcgtc gggctggttt acgtcgtcat cgtgctgttc
     6541 atcgcgatct ttgccaactg gattgcaccc tataacccgg cggaacagtt ccgcgatgca
     6601 ctgctcgccc cgccagcctg gcaggaaggc ggcagcatgg cgcacttgct gggcaccgat
     6661 gacgtaggcc gtgatatgct gtcgcgcctg atgtacggtg cgcgcctgtc gctgctggtt
     6721 ggctgtctgg tagttgtgtt atcgctgatt atgggcgtta ttctcggcct gatcgccggt
     6781 tactttggcg gcctagtcga taacatcatt atgcgcgtgg tcgatatcat gctggcgctg
     6841 ccaagtctgc tgctggcgct ggtgctggtg gcaattttcg gcccgtcgat tggtaacgcc
     6901 gcgctggcac tgaccttcgt tgccttgccg cactatgtgc gcttaacccg cgccgccgtg
     6961 ctggtggaag ttaaccgcga ttacgtcacc gcgtctcgcg tggcgggtgc cggggcgatg
     7021 cgtcagatgt ttattaacat cttcccgaac tgccttgcgc cgctgattgt tcaggcgtcg
     7081 ctcggtttct ctaacgccat tctcgatatg gctgctcttg gtttcctcgg catgggggca
     7141 cagccgccaa cgccggagtg gggcaccatg ctctccgacg tgttgcagtt cgcgcaaagc
     7201 gcctggtggg tcgtgacctt cccgggtctg gcgatcctgc tgacggtgct ggcatttaac
     7261 ctgatgggtg acggtctgcg tgacgcgctc gatcccaaac tgaagcagta agaggttcga
     7321 gatggcgtta ttaaatgtag ataaattatc ggtgcatttc ggcgacgaaa gcgcaccgtt
     7381 ccgcgccgta gaccgcatca gctacagcgt aaaacagggc gaagtggttg ggattgtggg
     7441 tgagtccggc tccggtaagt cggtcagttc cctggcgatt atggggctga ttgattatcc
     7501 gggccgcgta atggcggaaa aactggtgtt taacggtcag gatttgcagc gtatatcaga
     7561 aaaagagcgc cgcaacctgg tgggtgccga agtggcgatg atcttccagg acccgatgac
     7621 cagccttaac ccgtgctaca ccgtgggttt ccagatcatg gaagcgatta aggtgcatca
     7681 gggcggcaac aaaagtaccc gccgtcagcg ggcgattgac ctgctgaatc aggtcggtat
     7741 tcccgatccg gcttcgcgtc tggatgttta cccgcatcag ctttccggcg gcatgagcca
     7801 gcgcgtgatg atcgccatgg cgattgcctg tcgaccaaaa ctgctgattg ccgatgagcc
     7861 gaccaccgcg ctggacgtga ccattcaggc gcaaatcatc gaactactgc tggagctaca
     7921 gcagaaagag aacatggcgc tggtgttgat tacccatgac ctggcgctgg tggcggaagc
     7981 tgcgcataaa atcattgtga tgtatgcagg ccaggtggtg gaaacgggcg atgcgcacgc
     8041 catcttccat gcgccgcgtc acccgtatac tcaggcattg ctgcgtgcgc tgccagaatt
     8101 tgctcaggac aaagaacgtc tggcgtcgtt gccaggtgtc gttcccggca agtacgaccg
     8161 cccgaacggc tgcctgctta acccgcgctg cccctatgcc actgacagat gtcgcgctga
     8221 agaaccggcg ctgaatatgc tcgctgacgg gcgtcagtcc aaatgccatt acccacttga
     8281 tgatgccggg aggccgacac tatgagtacg caagaggcca ccctgcaaca accgctgttg
     8341 caggctatcg acctgaaaaa acattatccg gtgaagaaag gcatgttcgc gccggaacgt
     8401 ctggttaaag cgctggatgg cgtttcgttt aaccttgaac gtggcaaaac gctggcagta
     8461 gtgggcgaat ctggctgcgg taaatcgacc ctcggtcggt tgctgacgat gattgaaacg
     8521 cccaccggtg gcgagctgta ttaccagggg caggatctgc ttaagcacga tccgcaggcg
     8581 cagaagctgc gtcggcagaa aatccagatc gtcttccaga acccttacgg ttcgctgaat
     8641 ccgcgtaaaa aagtcgggca aattcttgaa gagccgctgc tgatcaacac cagcttaagc
     8701 aaagaacagc gtcgggaaaa agccctgtcg atgatggcga aagtcggcct gaaaaccgag
     8761 cactatgacc gctatccgca tatgttctcc ggcggtcagc gtcagcgtat cgccatcgcc
     8821 cgtggtctga tgctcgaccc ggatgtggtg attgccgatg aaccggtttc cgcgctggat
     8881 gtttcagtgc gcgcgcaggt gctgaatctg atgatggatt tgcagcagga gttggggctg
     8941 tcttatgtct ttatctccca cgacctgtcg gtggtggagc acattgctga tgaagtgatg
     9001 gtgatgtacc tgggccgctg cgtggagaag ggaacgaaag accaaatctt caataacccg
     9061 cgccatccgt acactcaggc gctactttcc gcgacgccgc gcctgaaccc ggacgatcgc
     9121 cgcgagcgca tcaagctcag cggtgaacta ccaagcccac tgaatccacc gccgggttgc
     9181 gccttcaacg cccgctgtcg tcggcgcttc ggcccctgca cccagttgca gccgcagcta
     9241 aaagactacg gcggtcaact ggtagcttgt tttgctgttg atcaggatga aaatccgcag
     9301 cgttaactca caaaaccggg gccatgcgct ccggtttact caatcagctt caacagtggc
     9361 gacaagcata gcaaaatgcc gtaaagcaga attagccagg ctttaaatcc gcgaagtttt
     9421 tccagttgtg cgactttata gatcaggaaa aacggaatga ggcacgacac aatgccatat
     9481 aacgggcttc ccaactgaaa gaacaccagc accgatacac gaaacgaaac ccaaatcgtc
     9541 aacgtaatga cgataaaagc gcagatcgcc agagtcagca tgcgtgagtt aattttcttg
     9601 gtatcaataa ttcggcttaa cagattgaga ataatgcctt taatagcctc gtggaaaccg
     9661 agataaatgc caaagaatgc ggtcagtacg gcaaagatat tcagcaccgt agaggtgata
     9721 tgaatgatat gtccagggat cacctgcgcg gccagtgcca gtgccgagat attttgttca
     9781 aaggcagaaa cggcttcttc gtggctaatt gagaaggtaa acgaaaaggc aaaaaacagg
     9841 atcaccgcga tgagcgtgat ataactaatt cggtgggtac gcagcgcgag ccgggttgcc
     9901 agtactttat ctgcttcccg tttacgatag gcaatattca ttgggttaag tacctgaata
     9961 aataccgcag aaaagaagca aaatggaatt gtaagcagga catcgcggaa aaagacggag
    10021 gcttgcggga aggcggttat attggcgaaa ttccagtgcg ggatcatcgc aaaaccgaac
    10081 acgacaataa tccctacttt gaccaccacc attggcccgg aaatcttaaa cagtaatcgt
    10141 tcaccaccag acgcaatcgc taccagtacg gcgaaaatag cgactttata aagtagagat
    10201 tgtgaaagat cggcatcggt taaaccgaag gttttcaggt acgaggcgct gtcgaaaacc
    10261 acggagagag agtagataaa aatcccgtgg ataatcatca aaaagtagat aaccccgagg
    10321 aaaattcccc agttcttccc caggtaatga ctgataatat cggtgtagtc attacaggaa
    10381 tcgctttcag aaagggtttt taaataaatg tcctgcacta cccaggtggc aggataagca
    10441 atgatcgctg cggtaataaa tacccaaatt cccttcaagc caatttgtac tggcatcagc
    10501 acggttccgg caccaatcgc catgcctatg cataataaaa cccagccgaa gtcgtagcgt
    10561 gtaaacggca atttctgatt gtgtttcgat agtgtgttgt gctgcataaa gtcctgtcca
    10621 gaaagaagtc ggcctcgcaa aaatgaataa acccagtttc tcttgcgcaa caggtggaga
    10681 agtcgaatac gcagtatgta aaaaatgagg caacagattc actggggagg tcgcgaggag
    10741 gcgcaattct acaaaattgc gcatttcact accagtctgg agtcagcccg gatatgaact
    10801 gcaactattg caaataaaga aattaagctg gaaggcatcg cgcaatgagg aagattgccg
    10861 ggtgctgaca gtaaaaagca atcgtaaaat cagcggcaag ccgaaagggt tccggtgagg
    10921 cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggagagag aaaacccccg
    10981 cacgttgcag gtatgcacct gacaacatca cgggggctaa tcttgaaccc aaacaactca
    11041 agaatagccg cgaaacgttg gcattacaac acaggcggct atatgacgct cgcagaactg
    11101 ggcatggcct tctggcatga tttagcggct ccggtcattg ctggcattct tgccagtatg
    11161 atcgtgaact ggctgaataa gcggaagtaa cgtgtcatgc gggcgtcagg ctgccgtaat
    11221 ggcaatttgc gcccggatca ggccgcagag gttaaactct gcggcctttt tcgtgcttac
    11281 tgcgggtaag gcacccagtc gccgccgttc aggcgaacgt acggtttatc ctggtattga
    11341 ataactactg catttgagtt ctcggagacc ggtgctgttt gtggcaaccc actggtgagt
    11401 tttttccagt caacattgtc ttcggtgaaa atcttgccat caagaacgcg aaccaccaga
    11461 tcggagatag ccaggaagct gctcggttgg tcgatgacaa tcggtgcccc ctgatgtggt
    11521 gccttcatgc cgaagaattt caccccaacg gggacgtctg tgatagacgg gctagggata
    11581 tcacgtaggc cagatacctg cattctgtcg cccttcagcg cgccgccgtg ttccggcacc
    11641 acgaccacca tcactttacg acccgatttc tccagttcag taaagaaggc gtccagttca
    11701 tcaaagaatt tctgcgcccg cgctttgtaa tccgctgttt tgctgacccc cggataatgg
    11761 ttgccgtcat gcagtggaag cgtgttgtag aacgtggcac tacggctgtt tttatctttt
    11821 tcggtaacgt ccagccagcg gttaagcacg gtggtatcgt cataaaccgg cgaaccatca
    11881 aagcccagca aaataaccgg cagatttgtt tgatccatca attcagtctg catgccgcca
    11941 ttttcgcgaa cttctttcaa aaaaccgccg aactggccgt tatgtcccat catcaggtgc
    12001 tgggtaaagc ccagtttcga aaggttatca aacagatagc agtcgttatt tgccggttga
    12061 tacagattag tgtgcgaagt ctgcccgcag ctggcgcgca gtaaacggat cgccgccggg
    12121 ccactgtagg aggtggcgga gttaaagttc ttgaactcaa tatcgaaatg cgaccacagt
    12181 ggatgcgaca tcaacccggc ggcttctata tccgaccagg aaagcgaaca gatgttaatc
    12241 accagtagtt caaatggctg agcatcagcg ggcagcgaag acgggaaggt cgatttacgt
    12301 ttcgcctccg cgttatagaa attattcagc caggcgttaa ggttcgccgt tgttggcggt
    12361 gtagtttgtg cgggcatatc acccactacc ggtgcgccac ccgtcgtctc aacggttgcc
    12421 gctgcgttac cacccgtcgt tgttacagtg gtcgtcggtt gtccggctgg ccacaaggag
    12481 aaacttggtc ccgccagggt aagtacgttc agccatagca gtatggcaac cacaaaaacg
    12541 gtaatgcgaa tccattgtga caggaataac caggccacta ataaaacaaa aatggcccca
    12601 atcatctgcc agttaataaa gcgtgtgaca aggtcgatta aataatcggt actgaacccc
    12661 gccacctgcg aaccctggct cattatgctt tccgggccag gcaaccaggt gtcatgccag
    12721 aacaaagcaa agccgatcgg cagggcaatc cagtggcgca agcgatgcag gctgtagcgc
    12781 ggaatgggca tcagcagaaa cgcggcaaac accaaattga ggagcggatg gaagttaaga
    12841 tatcccgccc acaacaggcc gaacttaacc agaaaataga agttccagcc ggaaaggccg
    12901 cgccagtatt gccagaggga agaaggcatg gcggtatttt gcgtaaattg agtcattttt
    12961 tggttgccct ggctttttcc gtgcggcgta atgtcccggc cggtttaaca taacgaggtt
    13021 tagcaaagaa caaacgtaag gtgtcgcgaa tgcgtcgcaa agagtgccgc gccagatagc
    13081 ccagcgggaa aaatatcagt gcgcaaacga caataatttc aatgatatcg ctgatggtca
    13141 tcatgatgag cgctccacag catcatctaa cagtcgcatg ggttctggta ttcgtcgcca
    13201 gtggcgacca tcgtgctcgg cattgatgac cggtttagaa ctttgcgtta aaggcagcgg
    13261 catgccccat tgttctgggg caagcaggcg catctgcacc agctcggcac tgatttgatc
    13321 atcttcaaac cagaccatac ggtttgagaa aatgtcgcca gtaggcaatg ggaaaatatg
    13381 attcaacgcg gtatccagat cgttaatccg acagaatgag agaaacagca ccagccgatt
    13441 accgccaatg gtcatgatat cgccggtacg gttagggcga cacagcgtca gggcttgttc
    13501 aacgcggata cccggtaccg gacgtagggc aaccagaacg cctttaccgt gggcaggtaa
    13561 tagagggtta ttcatcatgt tgttgacggc attacagaac acatcccact tctggaaacc
    13621 acgcagtttg agcggctggg tcattgacag caaggtagtg atatcttccg gcacatagcg
    13681 actaaacttc tgcccttgca cgctttcgat catcgtcaga caacgggaga gtggcgcatt
    13741 ccacggaata accatatttg caccgcaggc caataacaaa cgttcatcgg tggcgcgcag
    13801 gctagcggta ttttcccgca cgaggatttt catcgcacta ccgcgctggc gacgcagggt
    13861 atgaatgctg cgggccagtg gctcgatttg cgcattttgc tgtaaagaaa agaccaccgt
    13921 cgccgcctga gcggtacggg cttcattgaa caggacttcg ttattgttga acagttgcca
    13981 gtgttccgat agcggcggcg caccttccag tacagcaaca ttactcagaa tgcgtttttc
    14041 gtcgctgcgt ggttggatct ccgcctcttc gctttgaact aatgtccaga taccattttg
    14101 ttgctgaacg ctaagctgct gacgggcgct gacccctttt tcgttgcacc agaaggcaat
    14161 atccagcaaa tgttggtcac cctgaaaacg caaactggca agaccaaaaa gtgaacggta
    14221 ttcctcaagc aacaatgaaa attgtttatc gttattattt ccgggattaa ttaccaaaag
    14281 cgaacaatgg gttaacctgc tccatttatt cattttatcc aaccatgagc gaagccgctc
    14341 ggcaggaatg ttttgccatg cgttatttgc gcaaacaaga ataaaaaggt aattatgggg
    14401 atcaatagaa cactgcaaat cacgggtcaa atagtataga cctttttcat gatttagcat
    14461 tgaaaataat tttatttttt ccggcccttg ataatcatct aattgaaaga ttttcgccgg
    14521 atcgctgtcc atgctaatga ccgcgacgtg tgcggtttca gcctgggatg caattgtttg
    14581 attcgccaga ctgatagcat cttcatggcg atcgacgtta aaccaccaga cgccgcctgc
    14641 tggcatatgt cgcagctcat cccataatga tgagataccg atagagaata cagggcccac
    14701 aatgtccctc atgtcaccat tttgtctgat tatcagttta ctagcgaaag ctaagaaata
    14761 aacctaccat tgaaattaaa acttatcaga tttagcatgt gaatcaattt agaaggtcac
    14821 aagacgtgtg attttagtct tttcgttttt aactttttct gttgcgtaac ggaaagtcaa
    14881 aaagtgagca aattcccgtc tcgccgtaaa caataaaccg gcgaaatgct cacggttaga
    14941 attgtctcat gaacggtacg gttatttcat agggatcaag caaaatgaat aacaatgaac
    15001 cagatactct gcctgatccc gcgataggct atatcttcca gaatgatatt gtggcgttaa
    15061 agcaggcatt ttcactgcct gatattgatt atgccgatat ttcccaacgc gaacagttgg
    15121 ccgcggcatt aaaacgctgg ccgttgctgg cagagtttgc gcaacaaaag taggggattg
    15181 gtgaatggcc gtactgggat tgcagggggt gcggggaggc gtggggacaa caaccatcac
    15241 cgccgcatta gcctggtcat tacaaatgtt gggagaaaat gtcctggtgg tcgatgcctg
    15301 cccggacaac ttgttgcgcc tgtcatttaa tgttgatttt acccaccgtc agggctgggc
    15361 cagagcgatg ctggatggcc aggactggcg tgacgctggg ttgcgctaca cctcgcagct
    15421 cgatttgctg ccttttggtc agttatccat tgaagaacaa gaaaatccac agcacaggca
    15481 aacccggctg agcgatattt gctccggctt acagcaacta aaagccagcg ggcgttacca
    15541 gtggatttta atcgacttac cgcgtgatgc ctcgcagata acccaccagc tgctgagttt
    15601 gtgcgatcac tcgctggcaa tcgtcaatgt ggatgccaac tgccatatcc gactgcatca
    15661 gcaagcgctg ccggatggcg cacatatttt gattaatgac ttccgtattg gcagtcaggt
    15721 tcaggacgat atttaccagc tttggttgca aagccagcgc cgattactgc cgatgctcat
    15781 tcatcgtgat gaagcgatgg ctgaatgcct ggcggctaag caaccagtag gtgaatatcg
    15841 cagtgatgcg ctggcggctg aagagatact gacgctggcg aactggtgcc tgttgaacta
    15901 ctccgggctg aaaacgccag tcgggagtgc atcatgagta tcctgacccg gtggttgctt
    15961 atcccgccgg tcaacgcgcg gcttatcggg ggttatcgcg attatcgtcg tcacggtgcg
    16021 tcggctttca gcgcgacgct cggctgtttc tggatgatcc tggcctggat ttttattccg
    16081 ctggagcacc cgcgctggca gcgtattcgc gcagaacata aaaacctgta tccgcatatc
    16141 aacgcctcgc gtccgcgtcc gctggacccg gtccgttatc tcattcaaac atgctggtta
    16201 ctgatcggta catcgcgcaa agaaacgccg aaaccgcgca ggcgggcatt ttcaggtctg
    16261 cagaatattc gtggacgtta ccatcaatgg atgaacgagc tgcctgagcg cgttagccat
    16321 aaaacacagc atcttgatga gaaaaaagag ctcggtcatt tgagtgccgg ggcgcggcgg
    16381 ttgatcctcg gtatcatcgt caccttctcg ctgattctgg cgttaatctg cgttactcaa
    16441 ccgtttaacc cgctggcgca gtttatcttc ctgatgctgc tgtggggtgt agcgctgatc
    16501 gtacggcgga tgccggggcg cttctcggcg ctaatgttga ttgtgctgtc gctgaccgtt
    16561 tcttgccgtt atatctggtg gcgttacacc tctacgctga actgggacga tccggtcagc
    16621 ctggtgtgcg ggcttattct gctcttcgct gaaacgtacg cgtggattgt gctggtgctc
    16681 ggctacttcc aggtcgtatg gccgctgaat cgtcagccgg tgccattgcc gaaagatatg
    16741 tcgctgtggc cgtcggtgga tatctttgtc ccgacttaca acgaagatct caacgtggtg
    16801 aaaaatacca tttacgcctc gctgggtatc gactggccga aagacaaact gaacatctgg
    16861 atcctcgatg acggcggcag ggaagagttt cgccagtttg cgcaaaacgt gggggtgaaa
    16921 tatatcgccc gcaccactca tgaacatgcg aaagcgggca acatcaacaa tgcgctgaaa
    16981 tatgccaaag gcgagttcgt atcgattttc gactgcgacc acgtaccaac gcgatcgttc
    17041 ctgcaaatga ccatgggctg gttcctgaaa gagaaacagc tggcgatgat gcagacgccg
    17101 caccacttct tctcgccgga cccgtttgaa cgcaacctgg ggcgttttcg taaaacgccg
    17161 aacgaaggca cgctgttcta tggtctggtg caggatggca acgatatgtg ggacgccact
    17221 ttcttctgcg gttcctgtgc agtaattcgt cgcaaaccat tggatgaaat tggcggtatt
    17281 gctgtcgaaa cggtaaccga agatgcgcac acgtctctgc gcctgcaccg tcgtggctac
    17341 acctctgcat atatgcgtat tccgcaggcg gcggggctgg cgaccgaaag tctgtcggcg
    17401 catatcggtc agcgtattcg ctgggcacgc gggatggtgc aaatcttccg tctcgataac
    17461 ccgcttaccg gtaaagggct gaaattcgcc cagcggctgt gctacgtcaa cgccatgttc
    17521 cacttcttgt cgggcattcc acgactgatc ttcctgactg cgccgctggc gttcctgctg
    17581 cttcatgcct acatcatcta tgcgccagcg ttgatgatcg ccctgttcgt gctgccgcat
    17641 atgatccatg ccagcctgac caactcgaag atccagggca aatatcgcca ctctttctgg
    17701 agtgaaatct acgaaacggt gctggcgtgg tatatcgcac caccgacgct ggtggcgctg
    17761 attaacccgc acaaaggcaa atttaacgtc accgccaaag gtggactggt ggaagaagag
    17821 tacgtcgact gggtgatctc gcggccctac atcttccttg ttctgctcaa cctggtgggt
    17881 gttgcggtcg gcatctggcg ctacttctat ggcccgccaa ccgagatgct caccgtggtc
    17941 gtcagtatgg tgtgggtgtt ctacaacctg attgttcttg gcggcgcagt tgcggtatcg
    18001 gtagaaagca aacaggtacg ccgatcgcac cgcgtggaga tgacgatgcc cgcggcaatt
    18061 gcccgcgaag atggtcacct cttttcgtgt accgttcagg atttctccga cggtggtttg
    18121 gggatcaaga tcaacggtca ggcgcagatt ctggaagggc agaaagtgaa tctgttgctt
    18181 aaacgcggtc agcaggaata cgtcttcccg acccaggtgg cgcgcgtgat gggtaatgaa
    18241 gttgggctga aattaatgcc gctcaccacc cagcaacata tcgattttgt gcagtgtacg
    18301 tttgcccgtg cggatacatg ggcgctctgg caggacagct acccggaaga taagccgctg
    18361 gaaagtctgc tggatattct gaagctcggc ttccgtggct accgccatct ggcggagttt
    18421 gcgccttctt cggtgaaggg catattccgt gtgctgactt ctctggtttc ctgggttgta
    18481 tcgtttattc tgcgccgccc ggagcggagc gaaacggcac aaccatcgga tcaggctttg
    18541 gctcaacaat gatgataacg cgatgaaaag aaaactattc tggatttgtg cagtggctat
    18601 ggggatgagt gcgttcccct ctttcatgac gcaggcgacg ccagcaacgc aaccactgat
    18661 caatgctgag ccagctgtag ccgcccagac ggaacaaaat ccgcaggtgg ggcaagtgat
    18721 gccgggcgtg cagggcgctg atgcgccagt cgtggcgcag aacggtcctt cgcgtgatgt
    18781 gaagctgacc tttgcgcaaa ttgcgcctcc gccgggcagc atggtgctac gtggcattaa
    18841 cccgaacggc agcattgagt ttggtatgcg cagcgatgaa gtggtgacga aggcgatgct
    18901 caacctcgaa tacatgccat cgccatcgtt actgcctgtc cagtcgcagt taaggtttat
    18961 ctcaatgatg agctgatggg cgtactgcca gtgaccaaag aacagttggg taaaaaaacg
    19021 ctggcgcaaa tgcccattaa cccactgttt attaccgact tcaaccgtgt gcggctggag
    19081 tttgtcggcc attatcagga cgtgtgcgaa aacccggcca gaaccacgct ttggctggat
    19141 gttgggcgga gcagtggact ggatctgacc tatcagaccc tgaatgtgaa gaatgacctg
    19201 tcacacttcc cggtgccatt ctttgacccg cgcgataacc gtactaacac cttgccgatg
    19261 gtctttgcgg gtgcgccgga tgttgggctg caacaagcat ctgccattgt cgcctcgtgg
    19321 tttggttcgc gttctggctg gcgtgggcag aacttcccgg tgctctataa ccaactgccg
    19381 gatcgtaatg ccattgtctt tgcaaccaac gacaaacggc cggacttcct gcgcgatcat
    19441 ccggcggtaa aagccccggt gattgagatg atcaaccatc cgcagaatcc ttacgtcaaa
    19501 ctgctggtgg tgtttggtcg tgacgacaaa gacctgttgc aggcagcgaa aggtatcgct
    19561 cagggtaaca ttctgttccg tggtgaaagc gtggtagtga atgaagtgaa accgctgcta
    19621 ccgcgtaagc cgtacgatgc gccgaactgg gtacgtaccg atcgtccggt cacatttggc
    19681 gaactgaaaa cctatgaaga acagttacaa tccagcggtc ttgagccagc agcgattaac
    19741 gtttcgctaa acctgccgcc ggatctctac ctgatgcgca gtaccggcat tgatatggat
    19801 attaattacc gctacaccat gccgccggtg aaagacagtt cgcggatgga tatcagcctg
    19861 aataaccagt tcctgcaatc cttcaacctg agcagcaaac aggaggcgaa ccgcctgctg
    19921 ctgcggattc cggtattaca aggtttgctg gatggcaaaa cagatgtctc tattccggcg
    19981 ctgaaactgg gcgcgaccaa ccagctgcgc ttcgactttg agtatatgaa cccgatgccg
    20041 ggcggttcgg tggataactg tattaccttc cagccggtgc agaatcatgt ggtgattggt
    20101 gacgactcca ccatcgactt ctcgaagtat taccacttca tcccgatgcc ggatctacgc
    20161 gcctttgcta acgcgggctt cccattcagc cggatggcgg atctgtcgca aaccatcacc
    20221 gtgatgccga aagcgcctaa cgaagcacag atggaaacgt tgctgaatac tgttggtttt
    20281 atcggcgcac agacgggctt cccggcgatt aatctgacgg tgaccgatga tggcagcacc
    20341 attcagggca aagatgccga catcatgatc atcggtggta tcccggacaa actgaaagac
    20401 gataagcaga tcgacctatt ggtgcaggcg accgaaagct gggtgaaaac accgatgcgc
    20461 cagaccccgt tccccggcat tgtgccggac gagagcgatc gcgcggcaga aacccggtca
    20521 acgctgacct cttccggtgc gatggcggcg gtgattggct tccagtcgcc gtataacgac
    20581 cagcgcagcg tgattgcgct gttggcagat agcccacgcg gttatgaaat gcttaacgat
    20641 gcggtgaacg atagcggcaa acgcgccacc atgttcggtt cggtcgcggt gatccgcgag
    20701 tccggtatca acagcctacg tgttggcgac gtttattacg taggtcatct gccgtggttc
    20761 gagcgcgtgt ggtatgcgct ggcaaaccat ccgattctgc tggcggtgct ggcggctatc
    20821 agtgtgatat tgctggcatg ggtactgtgg cgtctgctgc gaattattag tcgtcgtcgt
    20881 cttaacccgg ataacgagta attgaagatg aatgtgttgc gtagtggaat cgtgacgatg
    20941 ctgttgttgg ctgcctttag tgttcaggca gcctgtacct ggcctgcctg ggagcagttt
    21001 aaaaaggatt acatcagtca ggaagggcgc gtcatcgacc ccagcgacgc gcgcaaaatc
    21061 accacctccg aagggcaaag ttacggtatg ttctttgccc tggcggctaa cgaccgtgca
    21121 gctttcgata atattctcga ctggacgcag aacaatctcg ctcagggttc tttaaaagaa
    21181 cgtttgcccg cctggctgtg gggcaagaaa gagaacagta agtgggaagt gctggacagc
    21241 aattcggcct ccgatggtga tgtctggatg gcctggtcgt tgctggaggc ggggcgtttg
    21301 tggaaagagc agcgttatac cgacatcggc agcgcgttgc taaaacgtat cgcgcgggag
    21361 gaagtggtga cggtgcctgg gctgggttcc atgttgttac cgggcaaagt gggttttgct
    21421 gaggataaca gctggcgttt taaccccagc tacctgccgc cgacgctggc gcagtatttc
    21481 acccgctttg gcgcgccgtg gactacgctg cgcgaaacca atcaacgttt attgctggaa
    21541 accgccccga aaggcttttc gccagactgg gtgcgttatg agaaagacaa aggctggcag
    21601 ctaaaagccg aaaaaacatt gatcagcagc tacgacgcca tccgcgttta catgtgggta
    21661 ggcatgatgc ctgacagcga tccgcagaaa gcgcggatgc tcaaccggtt taaaccgatg
    21721 gcgacattca ctgagaaaaa cggttatccg ccggaaaaag tggatgtggc tacggggaaa
    21781 gcgcagggta aaggaccagt cggtttttct gccgccatgc tgcccttttt acaaaaccgc
    21841 gatgcgcagg ccgttcagcg tcagcgcgtg gccgataact ttcccggcag cgatgcctat
    21901 tacaactatg tgctgaccct gtttggacaa ggctgggatc aacaccgttt ccgcttctcg
    21961 acaaaaggtg agttattacc tgactggggc caggaatgcg caaattcaca ctaaacatat
    22021 tcacgctttc cctcggtctg gccgtcatgc cgatggtcga ggcagcacca accgctcagc
    22081 aacagttgct ggagcaagtt cggttaggcg aagcgaccca tcgtgaagat ctggtgcaac
    22141 agtcgttata tcggctggaa cttattgatc cgaataaccc ggacgtcgtt gccgcccgtt
    22201 tccgttcttt gttacgttag ggcgatattg atggcgcgca aaaacagctc gatcggctgt
    22261 cgcagttagc gccgagttca aatgcgtata aatcgtcgcg gactacgatg ctactttcca
    22321 cgccggatgg tcgtcaggca ctgcaacagg cacgattgca ggcgacgacc ggtcatgcag
    22381 aagaagctgt ggcgagttac aacaaactgt tcaacggtgc gccgccggaa ggtgacattg
    22441 ctgtcgagta ctggagtacg gtggcgaaaa ttccggctcg ccgtggcgaa gcgattaatc
    22501 agttaaaacg catcaatgcg gatgcaccgg gcaatacggg cctgcaaaac aatctggcgc
    22561 tattgctgtt tagtagcgat cgccgtgacg aaggttttgc cgtcctggaa cagatggcaa
    22621 aatcgaacgc cgggcgcgaa ggggcctcta aaatctggta cgggcagatt aaagacatgc
    22681 ccgtcagtga tgccagtgtg tcggcgctga aaaaatatct ctcgatcttt agtgatggcg
    22741 atagcgtggc ggctgcgcaa tcgcaactgg cagaacagca aaaacagctg gccgatcctg
    22801 ctttccgcgc tcgtgcgcaa ggtttagcgg cggtggactc tggtatggcg ggtaaagcca
    22861 ttcccgaact acaacaggcg gtgcgggcga acccgaaaga cagtgaagct ctgggggcgc
    22921 tgggccaggc gtattctcag aaaggcgatc gcgccaatgc agtggcgaat ctggaaaaag
    22981 ccctcgcact ggacccgcac agcagcaaca acgacaaatg gaacagtctg ctgaaagtaa
    23041 accgctactg gctggcgatc cagcagggcg atgctgcgct gaaagccaat aatcctgacc
    23101 gggcagaacg cctgttccag caggcgcgta atgtcgataa caccgacagt tatgcagtgc
    23161 tggggctggg cgatgtggcg atggcgcgaa aagattatcc cgccgccgaa cgttattatc
    23221 agcagacctt gcgtatggac agcggcaaca ctaacgccgt gcgcgggctg gcaaatattt
    23281 accgccagca atcgccagaa aaagctgaag cgtttatcgc ctcgctctct gccagtcagc
    23341 ggcgtagcat tgatgatatc gaacgcagcc tgcaaaacga ccgtctggca cagcaggcag
    23401 aggcactgga aaaccagggc aaatgggcgc cggcggcagc acttcagcgg caacgactgg
    23461 cgctggaccc cggcagcgta tggattactt accgactttc gcaggatctc tggcaggccg
    23521 gacaacgcag ccaggccgat acgttaatgc gcaatctggc gcagcagaag tcgaacgacc
    23581 cggagcaggt ttacgcttac gggctgtacc tctctggtca tgaccaggac agagcggcgc
    23641 tggcgcatat caacagcctg ccgcgagcgc agtggaacag caatattcag gagctggtta
    23701 atcgactgca aagcgatcag gtgctggaaa ccgctaatcg cctgcgagaa agcggcaaag
    23761 aagctgaagc ggaagcgatg ctgcgtcagc aaccaccttc cacgcgtatt gacctcacgc
    23821 tggctgactg ggcgcagcag cgacgtgatt acaccgccgc ccgcgctgca tatcagaatg
    23881 tcctgacgct ggagccaact aacgccgatg ccattctggg gctgacggaa gtggatattg
    23941 ctgccggtga cacagcggcg gcacgtagcc agctggcgaa actgcccgct accgataacg
    24001 cctcgctgaa cacacagcgg cgcgtggcgc tggcgcaggc gcagcttgga gataccgcag
    24061 cggcgcagca gacgtttaat aagttgatcc cgcaggcaaa atctcagcca ccgtcgatgg
    24121 aaagcgcgat ggtgctgcgc gacggtgcga agtttgaagc gcaggcgggc gatccaaagc
    24181 aggcgctgga aacctacaaa gacgccatgg tcgcatccgg tgtgactacg acgcgtccgc
    24241 aggataacga cacattcacc cgactgaccc gtaacgacga gaaagatgac tggctgaaac
    24301 gcggcgtgcg cagcgatgcg gcgggcctct atcgccagca ggatcttaac gtcaccctcg
    24361 agcacgatta ctggggttcg agcggcaccg gtggttactc cgatctgaaa gcgcacacta
    24421 ccatgttgca ggtggatgcg ccgtattctg acgggcggat gttctttcgc agtgatttcg
    24481 tcaatatgaa cgtcggcagt ttctccacta atgccgatgg caaatgggat gacaactggg
    24541 gcacctgtac attacaggac tgtagtggca accgcagcca gtcggactcc ggtgccagcg
    24601 tggaggtcgg ctggcgaaat gacgtctgga gctgggatat cggtaccacg ccgatgggct
    24661 tcaacgtggt ggatgtggtc ggcggcatca gttacagcga tgatatcggg ccgctgggtt
    24721 acaccgttaa cgcccaccgt cggcccatct ccagttcttt gctggccttt ggtgggcaaa
    24781 aagactcccc gagcaatacc gggaaaaaat ggggtggcgt acgtgccgac ggtgtggggc
    24841 taagtctgag ctacgataaa ggtgaagcaa acggcgtctg ggcatcgctt agtggcgacc
    24901 agttaaccgg taaaaatgtc gaagataact ggcgcgtgcg ctggatgacg ggctattact
    24961 ataaggtcat taaccagaac aatcgccgcg tcacaatcgg cctgaacaac atgatctggc
    25021 attacgacaa agatctgagt ggctactcac tcggtcaggg cggttactac agtccgcagg
    25081 aatacctgtc gtttgctata ccggtgatgt ggcgggagcg cacggaaaac tggtcgtggg
    25141 agctgggggc gtctggctcg tggtcgcatt cacgcaccaa aaccatgccg cgttatccgc
    25201 tgatgaatct gatcccgacc gactggcagg aagaagctgc gcggcaatcc aacgatggcg
    25261 gcagcagtca gggcttcggc tacacggcgc gggcattact tgaacgacgt gttacttcca
    25321 actggtttgt tggcacggca attgatatcc agcaggcgaa agattacgca cccagccatt
    25381 tcctgctcta cgtacgttat tccgccgccg gatggcaggg tgacatggat ttaccgccgc
    25441 agccgctgat accttacgcc gactggtaag ttttcagata gcgcctctct taatgccgct
    25501 gcgatcgggt atactcgggc ggcaatctgg gatttccggg gggagacaat ttgcgcgtaa
    25561 gtcgctcgtt aacaatcaag cagatggcaa tggtggcagc cgttgtcctg gtgttcgttt
    25621 ttattttttg caccgttttg ctgttccatc tggtccagca gaatcgctat aacacggcta
    25681 cgcaactgga aagcattgct cgctctgtac gcgaaccctt atcttcagct attttgaaag
    25741 gcgatattcc cgaagcggaa gctattcttg ccagcattaa accggcaggc gtggtcagcc
    25801 gtgccgatgt agtgctgcct aaccagttcc aggcgctgcg taaaagtttt attccagagc
    25861 gcccggtgcc ggtaatggtt actcgcctgt ttgagctacc ggttcaaatc tcgctgggcg
    25921 tttactcgct cgaacgtccg gcaaacccgc agccaattgc ctatctggta ctacaggcgg
    25981 attccttccg tatgtataag ttcgtgatga gcaccctctc aacgttagtg accatttact
    26041 tacttttgtc gcttatcctg accgtcgcca tcagctggtg cattaaccgc ctgattttgc
    26101 atccgttacg caatattgct cgcgaactta acgccatccc agcccaggag cttgttggtc
    26161 accaactggc attaccgcgt ctgcatcagg acgatgaaat cggtatgttg gtgcgcagtt
    26221 acaacctcaa ccagcaattg ctgcagcgcc attatgaaga acagaacgaa aatgcgatgc
    26281 gcttcccggt gtcggatttg ccgaacaaag ccttgctgat ggagatgctg gagcaggttg
    26341 tcgcgcgtaa acaaaccacc gcgctgatga tcatcacctg tgaaaccctg cgtgatactg
    26401 cgggcgtgct gaaagaggcg caacgagaaa ttctgctgct gacgctggtg gaaaaactca
    26461 aatcggtact gtcgccacgt atgatcctcg cgcagattag cggttatgac tttgctgtca
    26521 ttgccaacgg tgtacaggaa ccgtggcacg caatcacctt aggtcagcaa gtgctcacta
    26581 tcatgagcga gcgcctgccg attgaacgta ttcaactccg tccgcactgt agcattggcg
    26641 tggcgatgtt ctacggcgat ctcaccgccg aacagcttta cagtcgcgct atttctgcgg
    26701 catttaccgc tcgccataaa ggcaagaatc agattcagtt ctttgatccg cagcagatgg
    26761 aagccgccca gaagcggttg acggaagaga gcgatatcct taatgcactg gaaaatcatc
    26821 agtttgctat ttggttacag ccacaggtcg agatgaccag cggtaaactg gtcagtgcgg
    26881 aagtgttact gcgtatccag caaccggatg gcagttggga cctgccggat ggcttaatcg
    26941 atcgcattga gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg gaagagtcct
    27001 gtcgattgct tgcagcctgg caagagcgcg gcattatgct gcccttgtcg gtaaacctct
    27061 ctgcgctgca actgatgcac ccaaatatgg tggcggatat gctggaactg ttaacccgct
    27121 atcgcattca accgggaaca ctgattctgg aagtgacaga aagccgacgt attgacgacc
    27181 ctcatgctgc ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg gtggcgctgg
    27241 atgatttcgg catgggctac gcagggctgc gtcagctgca gcatatgaaa tcgttgccaa
    27301 tcgacgtact gaaaatcgac aaaatgtttg ttgaaggctt gccggaagat agcagcatga
    27361 ttgctgcaat tatcatgctg gcgcagagcc tgaacttaca aatgattgcc gaaggcgtgg
    27421 agactgaagc acaacgcgac tggctggcaa aagcgggcgt tggtattgcc cagggcttcc
    27481 tttttgctcg cccactccct attgaaatct tcgaagagag ttacctggaa gaaaagtagc
    27541 taccccaaac tgattacaaa actttaaaaa gtgctggttt gtgcgagcca gctcaaactt
    27601 tttaaccttt ttgtttcaat tatgatccag gtacatttct gtgatgttgt ctgggtgtta
    27661 ttttaaggcc gcaggtacct cataacctta caagacctgt agttttacta aaggacaccc
    27721 tatgaaaacc tctctgttta aaagccttta ctttcaggtc ctgacagcga tagccattgg
    27781 tattctcctt ggccatttct atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg
    27841 cttcgttaag ctcattaaga tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg
    27901 cattgcgggc atggaaagca tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta
    27961 ctttgaaatt gtcagtacca tcgcgctgat tattggtctt atcatcgtta acgtcgtgca
    28021 gcctggtgcc ggaatgaacg tcgatccggc aacgcttgat gcgaaagcgg tagcggttta
    28081 cgccgatcag gcgaaagacc agggcattgt cgccttcatt atggatgtca tcccggtgag
    28141 cgtcattggc gcatttgcca gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt
    28201 tggttttgcg ctccaccgtc tgggcagcaa aggccaactg atttttaacg tgatcgaaag
    28261 tttctcgcag gtcatcttcg gcatcatcaa tatgatcatg cgtctggcac ctattggtgc
    28321 gttcggggca atggcgttta ccatcggtaa atacggcgtc ggcacactgg tgcaactggg
    28381 gcagctgatt atctgtttct atattacctg tatcctgttt gtggtgctgg tattgggttc
    28441 aatcgctaaa gcgactggtt tcagtatctt caaatttatc cgctacatcc gtgaagaact
    28501 gctgattgta ctggggactt catcttccga gtcggcgctg ccgcgtatgc tcgacaagat
    28561 ggagaaactc ggctgccgca aatcggtggt ggggctggtc atcccgacag gctactcgtt
    28621 taaccttgat ggcacatcga tatacctgac aatggcggcg gtgtttatcg cccaggccac
    28681 taacagtcag atggatatcg tccaccaaat cacgctgtta atcgtgttgc tgctttcttc
    28741 taaaggggcg gcaggggtaa cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc
    28801 ggtgggccat ttgccggtag cgggtctggc gctgatcctc ggtatcgacc gctttatgtc
    28861 agaagctcgt gcgctgacta acctggtcgg taacggcgta gcgaccattg tcgttgctaa
    28921 gtgggtgaaa gaactggacc acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga
    28981 tggcaaaacg cacgaattat cctcttaatc tcaccactta tgcccgcact cccttccccg
    29041 agtgcgggtg tttgcgcata attgacccct gtgcctgaac ttaccacctg ggtgagttca
    29101 ggtttatttc acttcttccg tgacattttc atgttcttgc ggtctaacac gaagtgtttt
    29161 taacgtcatt tagactgact gacaacgggc agttttttat taccaggata gttgatcagg
    29221 ggttcacatg cagggcacaa aaattcgact tttagcgggc ggtttgctga tgatggccac
    29281 tgctggctat gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct
    29341 ttccaacggt ttacagtggc aagtgctgac caccccccag cgtcccagcg atcgtgttga
    29401 aattcgcctg ctggttaata ccggttcgct cgccgaaagt acacaacaga gcggttacag
    29461 tcacgccatc cctcgtattg cgctaacgca aagcggtggc cttgacgcag cacaggcgcg
    29521 ttcattgtgg cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta
    29581 tgacaccacg ctgtttaatc tgagtttgcc caataaccgt aacgatttgc tgaaagaagc
    29641 gctctcttat ctggcaaatg ccactggcaa attgaccatc acaccagaaa ccatcaacca
    29701 cgcgctgcaa agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg
    29761 gcgctatcgt ctgaaagggt caaccttgtt aggtcacgat cctgccgatc cgctgaaaca
    29821 acccgttgaa gcggaaaaaa ttaaagattt ctatcagaaa tggtacaccc cggatgcaat
    29881 gacgctactg gtggtgggaa acgtggatgc gcgctcggtt gtcgaccaaa tcaacaaaac
    29941 gtttggcgaa ctgaaaggca aacgtgaaac gccagctccg gtgccgacgc tttctccgct
    30001 gcgtgcggaa gcggtgagta ttatgactga cgcggtgcgt caggaccggt tatctatcat
    30061 gtgggatacg ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc
    30121 ggacctggcc cgcgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtaacag
    30181 caaagacatc ggtcttggat ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat
    30241 caacatcgaa tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact
    30301 ggcgaaggtt cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa
    30361 gaaactggag ctgcagaaac tgtttgccgc ctatgcgcgg gctgataccg atattctgat
    30421 gggtcagcgg atgcgttcgt tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca
    30481 gaaactgcgg caggatttcc ttaacagcct gacggtggag atgttaaatc aggatctgcg
    30541 tcagcagttg tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt
    30601 taacatgaaa gcgttgcagg cggcctggga tcaaatcatg gccccatcga ctgctgctgc
    30661 cgccacctct gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca
    30721 gtaatgcctg atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag
    30781 aggggtaaca ctttatccag ccttttgcat atgctgcgtt tacgctggca tcgcctcacg
    30841 cgggataatc gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc
    30901 cgcgttttcc gcactgccgc ccgtcaaacg taccgccaga taaccggcgc tgaaagagtc
    30961 gccagctgcg gtggtatcga tcactttttc tttcggcagt ttcaccgccg gaacatccac
    31021 taacccttcg ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc
    31081 tttcacgccc gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag
    31141 cgcgtcttca tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgttggta
    31201 cacctgctgt gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat
    31261 cacttttccg ccgttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt
    31321 cgggcttaag atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc
    31381 ttcgcaaatc gccgtagact gctcactctc cagccagaat ttggcggcgg cttcgttccg
    31441 ccagtagtag aacgtacgct cgccggtgct gtcggtttca atgtagtaaa ggcccggcag
    31501 acggttttcc atccgttggg tcagggaaat atcaacgttc tcgccgtgcc aggcgtccag
    31561 catctgctgg ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc
    31621 aggatcgacc tgacgggcga tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg
    31681 cttaacgtcc gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt
    31741 tttggacata gtcgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt
    31801 ttgctggtca acaatattaa aacagtgttc cattattttt ttgagccaga acgtattcct
    31861 gaaagattcc gttgtggagt gaggaaattt accccggcca ggcggtaaag ttctgccctt
    31921 acgcgccgat aatctttgtc gagtccgggc agcatcactt ttaaacacag gacatctttg
    31981 atgataaggc aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag
    32041 gaacggcgtt tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca
    32101 tgcgggcggt taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg
    32161 caacgcctgc cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt
    32221 gtgaaagagc agattgattt gctggcgcaa aaagccgact tctttataga gcacggcctg
    32281 ctggcatcgg tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc
    32341 ctgcgccaaa ttgagcgtct tccctggctg cgtttcgaac tggtggagca tatccgtctg
    32401 ccgaaagatt caacctttgc ctcgatgtgt gaatttggcc cgctgtggct gggtgatttt
    32461 ggtaccggga tggcaaattt ctctgcacta agtgaagtgc gttatgacta catcaaaatc
    32521 gcgcgagaac tgtttgtgat gctgcgtcag tcgccggaag gacgcacact cttttctcag
    32581 cttttacatc taatgaatcg ctattgtcgc ggggtgattg ttgagggcgt agaaacgccg
    32641 gaagagtggc gtgatgtaca gaactcgccc gcattcgccg cacaaggctg gtttctttca
    32701 cgcccggcac cgatagaaac gctgaatacg gcggttctgg cgctataagc tgcctcattt
    32761 tccgcctggc tggactatct ttaggactgg cacaggaaga atatgaggaa gcgaacgatg
    32821 agcaaggcag gcaaaataac tgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg
    32881 gcgatcattt tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa
    32941 gtctctgcgg agttgaatcg tccgttcgct atccgtggcg atctgggcgt ggtgtgggag
    33001 cggcaaaaac aagaaactgg ctggcgcagc tgggtgccgt ggcctcatgt acatgcagaa
    33061 gacatcattc ttggcaatcc accggatatt cccgaagtca cgatggtgca tttgccgcga
    33121 gtagaggcaa cgctggcacc gctggcgctg ctgaccaaaa cggtctggct gccgtggatc
    33181 aagctcgaaa agcccgacgc gcgcctgatt cgcctctccg aaaagaacaa taactggacg
    33241 tttaatctcg ccaacgatga taacaaagac gcgaatgcaa agccgtcggc atggtcgttt
    33301 cggctggata atattctttt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa
    33361 gcggatctgg aaatttttgt tgatccgtta ggcaagccgc tgccgttcag cgaagttact
    33421 ggatcgaaag gtaaagcgga taaagaaaag gtgggcgatt acgtttttgg cctgaaggcg
    33481 cagggacgtt ataacggtga acagctcacg ggtacgggaa aaataggcgg tatgctggcg
    33541 ctgcgtggcg aagggacgcc gtttccggta caggctgatt tccgttcagg taatacccgt
    33601 gttgcttttg atggcgtcgt gaatgaccca atgaagatgg gcggtgtcga tttacggctt
    33661 aaattttctg gcgattcact gggtgatctc tatgaactga cgggcgttct gctgcccgat
    33721 accccgccgt ttgaaacgga tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg
    33781 gtctttgatt atcgcggttt taatgggcga attggcgata gcgatatcca cggttctctg
    33841 gtctacacca ccggaaagcc gcgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg
    33901 cggctggcgg acctgggacc gttgattggc gttgattcgg ggaaaggggc agaaaagtcg
    33961 aaacggtctg aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct
    34021 tatgaccgct tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggc
    34081 cgacgtatcg agcatggcag tagcctgccg attagcgatc tttctactca tatcatcctc
    34141 aaaaatgctg acctgcgcct gcaaccgctg aaatttggca tggcgggcgg cagcattgcg
    34201 gcgaatatcc atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct
    34261 cgtcgactga aactgaaaga actgatgccc gatgtggaac tgatgcagaa gacgctgggg
    34321 gaaatgaacg gtgacgcgga actacgcggt agcggtaact cggtggcggc gcttttaggc
    34381 aacagtaacg gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg
    34441 gagattgttg ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgacgatgag
    34501 gtgcgggtga actgcgcggc ggcgaatctg aatattgcca acggcgtagc gcgcccgcag
    34561 atttttgctt tcgatactga gaacgcgttg attaatgtta ccggcacggc aagttttgct
    34621 tcggaacagc tggatttgac tattgatccg gagagtaaag ggattcggat tatcacactg
    34681 cgttcgccgc tgtatgtgcg tgggacgttt aaaaatccgc aggctggggt gaaagccgga
    34741 ccgctgattg cccgtggtgc cgttgcggcg gcactggcaa cgctggtaac accggcggcg
    34801 gcgttattgg cactgatctc accttccgaa ggggaggcta atcagtgtcg gacgatattg
    34861 tcgcagatga agaagtgatg aggttggatt gtgtttgctg gtgagttcgg gagcagcgtg
    34921 ttcccctcac cctaatcctc tccccaaagg ggcgaggaga ctgttttgtg cgtgttgaaa
    34981 caacgagtac cgcttttccg ttcactttct gggggagggc tgttatgttc gcgatataac
    35041 atctcgctcc gcttttctcc ctctcccttt cagggagagg gctggggtga gggtggctgc
    35101 ccacacgata cttacaacga ctgatgttgc gtctcatggg tcagcagcag ggcgattaac
    35161 gtcaagccag ccatcgccgc cagatataac cccaccgcac ctaacccgta gttagtctgc
    35221 aaccaggctg cgatatacgg cgcaacggaa gccccgagaa tcgacgatac gttgtaagag
    35281 aacgatgctc cggtgtaacg cacttctgtc gggaacagct ctggtaacag cgcgcccatc
    35341 ggaccgaagg tcagccccat cagacttaac cccagcagca ggaaggcaaa aaccagaatc
    35401 gggttgccag aaccgagcaa agggttaaag gcgaatagcg cgaacaggat gagcagcgtg
    35461 gtgatgatta ccatgctttt acggcgacca aaggcatcag ccagtaatcc agcgactggc
    35521 accatcacgc caaaaccaat aactgccatc atcaacatcc acaacacttc gttacgcggc
    35581 aggccaagcc caactggcgc ggcggcggta ctaaaggtca tggagtagac cgtcatgatg
    35641 taaaacagcg tataggttgc cagcataatg aacgtaccca gtacggttac gcgaacatgt
    35701 ttggtcagca gcgtacccag cgggatcttc acctgttttt tcgctttagc gactttctca
    35761 aacaccggcg actcatgcag cgacacgcga acatacaggc cgataatgac cagcaccgcc
    35821 gagaagataa atggcacacg ccagccccag ctcataaact gctcgtcggt cagtagccag
    35881 gaaagcagca agaaagtgcc gttggcaaag aagaagccaa tcggtgcgcc cagctgcggg
    35941 aaggagccgt acaatgcacg tttgcgcggt ggggcgtttt cagtcgccag cagcgccgcg
    36001 ccgccccatt caccacctaa gcccagaccc tgaccaaatc gagccagcgc cagcagcagc
    36061 ggggcgaaaa taccaatcgt ggcatagcct ggcagcagac caatcaccac ggtcgaaatc
    36121 cccatcgtta gcaacgaggc gaccagcgtc gctttacgcc caacgcgatc gccaaaatga
    36181 ccaaaaacgg cagagccaat ggggcgcgcg acgaaggcga tggcgaaggt ggcgagcgac
    36241 tgtagcgttg ctgccgtagg atcgccctgc gggaagaaga tatgcggaaa cacaataacg
    36301 gccgcagtgg cgtaaatgta aaagtcgaag aactcaatgg ctgtgccaat gagagaggcg
    36361 acaaggactt tattacgcga gttgatcggc gtgtattctt gctcgtggtc gagtgttgtg
    36421 gctgttgctt gcataatctt ttcttatttt ttggctaacg aatagccata ttacgcacag
    36481 caaaagcgat atttcaatgt gtaacaaatc gcggaaatgg tcaaaaacca ggcaaaaagg
    36541 ggataatgtt taggccagtg atttttcttc tatgaaatgc ttcacataat tttataaata
    36601 gtggataaaa ctggtttcag gttaaataaa agttaccaac tggatttata tgctggggtt
    36661 atgagttaga atgaaattgt ttgctcccaa atagatggct gaagaggctc tggggacgag
    36721 tttcaagagg ttatgtgcgc attatagccg gatgcgatgc tggcgcatct tatccggcct
    36781 acggggagcc aggccgaata aaatcgtatg ccggataagg cgtttacgcc gcatccggcc
    36841 tattttaagg ctgcgttttc cctagcattc gcgggtcgtc tttatattcg gcggtggcaa
    36901 tccacgccgc gcaaaacagc gtcaaacggg cgaagaagta gaaaaatgcc atcagcccca
    36961 gcacggaacc aaatgctgcg ccagaggggg atttcatcag cgatggcagg gtgtaggtca
    37021 tcacgatttt aatcacttca aaaccaatag cagccagaaa tgttccgcga atcagggctt
    37081 ttttgcgcgg acggtggcgc ggcaaccgcc agaagatcca gaagaaaagc agatagttgg
    37141 cgaagatgga aatcgccagg ccaatcaatc gccacgtcgg tttcagccac tcaatgctgt
    37201 tcaggtgcag ggcgctaata atcatttgct gcgccgaacc ggcaaccgag gtgatcgaaa
    37261 gcgtcacaat cagcgcaatc aacaaaccaa tcagtgaaat aaaatcacgc agatatttta
    37321 cccagaactt ctcctgatct tgcggcgagc gttcccagac atcacgcgac tgggcacgaa
    37381 tcgcttcacg caggttaccc atccagttga tgccggaata aagtgccacc gccaggccga
    37441 caagccctac agtcgtacgc tgctgaacgg cggtgttgat ggtgtttttc aacgtggcgg
    37501 ctagtgtcgg atcgctgatg ttttgcagaa ttttgtcgaa gatatcctgt agcaacatcg
    37561 gatgggaggc cagcacaaag ccccctgcgg caaacgacac catcaaaatc gggatcatcg
    37621 acaaaaatga gaaataggtg atagccgcac caaactggtt acccagccga tcgttaaagc
    37681 gttctgtcgc gcgaatcagg tgcgcaataa ccggttggcg ttggactttt tcggcagtgg
    37741 tggtgacggt ttccagcgcc tggctaactt tgccgccttt ctcgctatta tccaactgct
    37801 taatcggctc gagctccaga tcctgggtgg gacgtttgat ctcgttttcc tgcgtcatcc
    37861 gctcttttgt ccttctgtcg tggtcattct taaaaagtat agtcggtcag tccaggtgct
    37921 ctttcattac gccagccagc cactgcataa acaggtttac acgccgggaa agctcccgac
    37981 gctgcgggta aaccagcgaa agggagagcg gctcggcacg gtagccaggt aatacttcaa
    38041 taagccgccc ggcacgcagg gcttcgcgca cggcaatgcg cgggatctga ataatcccca
    38101 gaccggcaag accggcggcg agatagtttt cgctactatt taccgtcaac atgccgccag
    38161 acttaaacca ctggacgcca ttaacgctgg caacctcaaa acctaacgga tgtacaccca
    38221 ggtgcggtgt ataacgcact attgcgtgtg aagtcagatc atcggggctt tgcggataac
    38281 caaagcgcgt cagatagtgc ggactggcac agttgaccat ggtcagtttg ccgaggggac
    38341 gggcgataac gccatcttcc ggtaacgcgc cagtgcgtat cacgcaatca aaaccatcat
    38401 gaagaatatc taccggacgg tcatggctac tcagttccag ctcaattccc ggatgcagat
    38461 agagaaattc cgacaggcgc ggcagtaaca ggtttttcgc gattcctggc gggatgtcga
    38521 tgcgtaattt accgctgata ctggtggcat cctgttgaaa cagaccgtcc agttcgttga
    38581 gattactcaa cacatctttt gcccgttgat aataggtcat gccttctggc gtgagtttga
    38641 cccgtcgcgt ggtgcgctgg agaagctggg tgccaagttg atgttccagt gcctgtatct
    38701 ggcgcgaaac acttcccttt ggcaaagcaa agaaatccgc tgcgcgggaa aaactttcca
    38761 gctccgcgac tttgatgaac aactgcattg cgtgaatttt atccatttct ttgaccgatt
    38821 gttgttcaca gcgaaacagt aacgcgcata tcgcattgtt tattgttttt tctaacatct
    38881 aatgccgggt ttcacaatgt tgtcatgacg aatgtggcga tttctcagca ggaaaagggc
    38941 ggaatagctt tatgagaaag gtgaatcccc ttaaacagag cgtatttttc agtgattccc
    39001 tttaaagtga atagaggacg tattggctat ttgtgcttat ttttgtctca ttgccatttg
    39061 ttattttggc ttgtagtcgc ggaatttcat ttttaattgc atgcgtacta accaccaata
    39121 taaggaaatc ctcaaaattg ttttaaatag tgccctcgac atgtaatttc aggcgtttag
    39181 ctgtaactat ttatatcggt aactattttc tacgcgtcat ctctgtagaa tggccaccgc
    39241 caaatggcga attcttattt tttctcgcgg gtaataccct tccactactt aagtcttttt
    39301 aacatattca ggtggagatt cagttatgca aatagtcatg tttgacaggc agtcaatatt
    39361 tattcatgga atgaaaatca gtttacagca gcgtattcca ggggtgagta ttcagggggc
    39421 cagtcaggca gacgagttat ggcaaaagct ggaaagttac cctgaagcct tagttatgct
    39481 cgatggcgat caggatggtg agttttgcta ctggttgctg caaaaaaccg tggtgcagtt
    39541 tcctgaggtt aaggtgttaa tcacggcgac ggattgcaat aaacggtggt tacaggaagt
    39601 tatccatttt aatgtgctgg ccattgtgcc tcgtgattca accgtcgaga catttgcgtt
    39661 ggcggtaaat agtgcggcga tggggatgat gtttctgcct ggagactggc ggacgacgcc
    39721 ggaaaaggac attaaagatc tcaaatcgtt aagcgcccgc cagcgggaga ttttaaccat
    39781 gttagcggcg ggcgagtcaa ataaggagat cggcagagcg ctgaatatca gtaccggaac
    39841 ggtaaaagcg catctggaat ccctgtatcg ccgtctggaa gtgaaaaatc gcacccaggc
    39901 ggcaatgatg ttaaatatct cctcctgaag aggataattt tacagccagc aggaagttag
    39961 cggctggctg taaaaatatt atggttcgcc gtacaaacca attaaacggc gtaccacacc
    40021 gttagtccag ccaaacccat cctgcaacgg atactcgcca ccgccgcctt cgcgtggaac
    40081 accatcggca atatggtatt tttcgatcag tttgtgctgt tccagataga actgattcac
    40141 cgtcttcagc cagcttcgcg cgatttcatc acccagaagg tcatcgccgt acattttaaa
    40201 tccctgaatc gccatccatt gtaacggtgc ccagccgttg ggtttatccc actgttcacc
    40261 ggtttcgtac tcgcttgcca gaatcccgcc aggtgtcagt aaccgactgc gcacggcatt
    40321 tgccagacga tcggcctgtt catggttcgc cataccgaca tagagtggca caatggcggc
    40381 agcggaaaac agcgccagtt gttcgcgtcg ccagtcgtaa tcgcggtaga tgccgttttc
    40441 atcatcccag aggtaacgct ttaccgcatc gcgacgggca ctggccttct ggcggaacag
    40501 tgcttctgtc tctttctcgc ctttcagcgc cgagatgttg gcgatggcgc tctccagttt
    40561 aaacaggaag gcattcagat cgatggggat gaactgggtg gtacgaatac tcgccagacg
    40621 accagtatca cgcagccaac gggaagagta atcccaaccg gaggccgccc ccgctcgtaa
    40681 atcgcggtac acctcgttgg gcgggcgacc agaatgtttc gcggtttcaa cgtcctcaag
    40741 ccaggattcg tcacgcggcg tgtcgcgatc gtcccagtaa cggttgagca gcgatccgtc
    40801 cggcatccgc acaacatggc gataggcctg attagggatc aacgattctg caccgtccat
    40861 ccagaaggca tattccattt tcaggtggtc gagatagcgg cgcgcaccgc gtacaccatc
    40921 ttcttcaaac aactccacca tcagcgcaaa aaccggtggt tgcgagcggc tcaaataata
    40981 ggtgcggttg ccgttgggga tgtgaccgta gttttcgatc atccaggcga agttatcggc
    41041 catgcatttc agcaaatctt cccgaccact ttccgccagc cccagcatgg tgaaatagga
    41101 atcccaatag taggtttcgc taaaacggcc gcccgggaca atatatgact gcggtagcgc
    41161 cagcagagaa gaccacggaa tgtgatcctg tggttcgcgg gttagcaccg gccacagctg
    41221 gtcgatatgc tctttcaggg aattttgcgg gtccgataca tactcgctgg agtagacctc
    41281 cggcagccag aagtgatttt caacaaactt gcgcaagtca aaatcacgat gacggcgcac
    41341 tttacggtag cggattaaaa tatccagcgg gtccattttg ggtgcgcagt cgggaaaggt
    41401 tttactgtcg gggaaaattt tcgccgactg aacatgctcg aacagttcga gatagcgatc
    41461 ggcaggcgtc agcgcatcag aggcgggcag cccttcaatc atctcgggtt ccggttctgc
    41521 ctcgatcatc tcatccagtt ttaattcata cgggtccagt tcatagcaga gatcgacttc
    41581 gatcatcagt tcgtctggat tagggttttg aattttctga ttgagcataa ggatccaacc
    41641 tccgaaattc gtcgctaatt atcggctttt ctttaagggt agtcattcgc tccgcctggt
    41701 gggcgacaaa gcgtgcggtg gatcacgttt attaacttgt caaaaaatat aacgcactaa
    41761 tatcaatgga atttataaaa atacggattt ggtgttgttt ttcattttca gaacattcaa
    41821 tcctgctctc gccgcaatca tgatatgcca ttcctgatct gggtaatagg cacaggctat
    41881 cttattgata gtttatattc atgtaattga ttgattccta tcaaaaagcg tatctccctg
    41941 ttttttatga tggctttgtc tcgcagagac gtttgcagca ttctggaaaa ttttctgggt
    42001 cacaataact caccaccgtg agggaagaat aatgaaaatg gtctcacgta ttaccgcgat
    42061 cggcctggct ggcgtcgcga tttgctattt agggttatct ggttatgtgt ggtaccacga
    42121 taataaacgc agtaaacagg ccgatgttca ggcatctgct gtcagtgaaa ataataaggt
    42181 tttaggcttt ctccgcgaaa aaggatgcga ctattgccac acgccttcgg cagaattacc
    42241 cgcctattat tatattcctg gcgcgaaaca gttgatggat tacgacatta agcttggata
    42301 taaatctttt aacctcgagg ccgtgcgtgc ggcactgctg gctgataaac ccgtttcgca
    42361 aagcgatctg aataagattg aatgggtgat gcagtatgaa actatgccac caacgcgtta
    42421 taccgcgcta cactgggcgg gtaaggtgag tgatgaagag cgggcggaaa tactggcctg
    42481 gattgcaaaa cagcgcgcgg aatattacgc cagcaatgat actgctccgg agcatcgcaa
    42541 tgaaccggtg cagcccatcc cgcaaaaact gcctaccgat gcgcaaaaag tggcgttggg
    42601 ctttgcgctg tatcacgatc cccgtttatc ggctgatagc accatttcat gcgctcattg
    42661 ccatgcgttg aatgcggggg gcgtcgatgg cagaaaaaca tcgattggtg ttggtggcgc
    42721 agttgggccg attaacgcgc cgacggtatt taactcagta tttaacgttg agcagttctg
    42781 ggatggtcgt gcggcaacat tgcaggatca ggcaggtgga ccgccgttga acccgattga
    42841 aatggcgtcg aaatcctggg acgaaattat tgctaagctg gaaaaagatc cgcagcttaa
    42901 agcgcagttc ctcgaagttt atccgcaagg tttcagtggc gaaaatatta ctgatgccat
    42961 tgctgaattt gagaaaacat taattacgcc ggattcccca tttgataaat ggttgcgtgg
    43021 agatgaaaat gcgctgacgg cgcaacagaa aaaaggctat caattattta aagataataa
    43081 atgtgcaact tgtcatggtg gtattattct cggcggacgt tcctttgaac cgttggggct
    43141 gaaaaaagac tttaactttg gggaaattac ggcggcggat attggtcgta tgaatgtgac
    43201 taaagaagag cgtgataaat tgcgccagaa agtacccggt ttacgtaacg ttgctttaac
    43261 ggcaccgtac ttccatcgcg gtgacgtgcc gacgctggac ggggcggtga aactgatgct
    43321 gcgctatcag gtaggcaaag agctgccgca ggaggatgtg gatgatatcg tagctttcct
    43381 gcacagtctg aacggggtgt acacgccgta tatgcaggat aaacaataac taatttgatc
    43441 gcccgaacag gaatgtttgg gcgattttta ttacgataat aaagtctgtt tttaatatta
    43501 tcatgttaaa tgtttatatt ataaaaagtc gtttttctgc ttaggatttt gttatttaaa
    43561 ttaagcctgt aatgccttgc ttccattgcg gataaatcct acttttttat tgccttcaaa
    43621 taaatttaag gagttcgaaa tggaccagaa gctgttaacg gatttccgct cagaactact
    43681 cgattcacgt tttggcgcaa aggccatttc tactatcgcg gagtcaaaac gatttccgct
    43741 gcacgaaatg cgcgatgatg tcgcatttca gattatcaat gatgaattat atcttgatgg
    43801 caacgctcgt cagaacttgg ccactttctg ccagacctgg gacgacgaaa acgtccataa
    43861 attgatggat ttgtcgatca a
//
LOCUS       DAJTNN010000009        42030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTNN010000009 DAJTNN010000000
VERSION     DAJTNN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.009"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..610)
                     /gene="yeeE"
                     /locus_tag="OO150_000483"
                     /pseudo
     CDS             complement(1..610)
                     /gene="yeeE"
                     /locus_tag="OO150_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
     gene            complement(789..2147)
                     /gene="plaP"
                     /locus_tag="OO150_000484"
     CDS             complement(789..2147)
                     /gene="plaP"
                     /locus_tag="OO150_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5514739.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYRVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="OO150_000485"
     CDS             complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="OO150_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5514740.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(2414..3343)
                     /locus_tag="OO150_000486"
     CDS             complement(2414..3343)
                     /locus_tag="OO150_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5514741.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(3389..4213)
                     /locus_tag="OO150_000487"
     CDS             complement(3389..4213)
                     /locus_tag="OO150_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5514742.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGLVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="OO150_000488"
                     /pseudo
     CDS             complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="OO150_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
     gene            complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="OO150_000489"
     CDS             complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="OO150_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5514743.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            5081..5131
                     /gene="hisL"
                     /locus_tag="OO150_000490"
     CDS             5081..5131
                     /gene="hisL"
                     /locus_tag="OO150_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5514744.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    5107..5230
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            5277..6176
                     /gene="hisG"
                     /locus_tag="OO150_000491"
     CDS             5277..6176
                     /gene="hisG"
                     /locus_tag="OO150_000491"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5514745.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            6182..7486
                     /gene="hisD"
                     /locus_tag="OO150_000492"
     CDS             6182..7486
                     /gene="hisD"
                     /locus_tag="OO150_000492"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5514746.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTEDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSLESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            7483..8553
                     /gene="hisC"
                     /locus_tag="OO150_000493"
     CDS             7483..8553
                     /gene="hisC"
                     /locus_tag="OO150_000493"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5514747.1"
                     /translation="MSIVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYGVSAETIGVECRTVPTLDNWQLDLQGISDKLEGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     ECLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            8553..9620
                     /gene="hisB"
                     /locus_tag="OO150_000494"
     CDS             8553..9620
                     /gene="hisB"
                     /locus_tag="OO150_000494"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5514748.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAIDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9620..10210
                     /gene="hisH"
                     /locus_tag="OO150_000495"
     CDS             9620..10210
                     /gene="hisH"
                     /locus_tag="OO150_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5514749.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            10210..10947
                     /gene="hisA"
                     /locus_tag="OO150_000496"
     CDS             10210..10947
                     /gene="hisA"
                     /locus_tag="OO150_000496"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5514750.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10929..11705
                     /gene="hisF"
                     /locus_tag="OO150_000497"
     CDS             10929..11705
                     /gene="hisF"
                     /locus_tag="OO150_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5514751.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEVVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11699..12310
                     /locus_tag="OO150_000498"
     CDS             11699..12310
                     /locus_tag="OO150_000498"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5514752.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVAFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLLLVNPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(12406..13383)
                     /gene="wzzB"
                     /locus_tag="OO150_000499"
     CDS             complement(12406..13383)
                     /gene="wzzB"
                     /locus_tag="OO150_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5514753.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(13530..13622)
                     /locus_tag="OO150_000500"
                     /pseudo
     CDS             complement(13530..13622)
                     /locus_tag="OO150_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            13792..14796
                     /locus_tag="OO150_000501"
     CDS             13792..14796
                     /locus_tag="OO150_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HCR5514754.1"
                     /translation="MKFLVTGAAGFIGFYLSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFERVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(14862..16028)
                     /gene="ugd"
                     /locus_tag="OO150_000502"
     CDS             complement(14862..16028)
                     /gene="ugd"
                     /locus_tag="OO150_000502"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR5514755.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKRQSP
                     LVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSERAKRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKNGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(16277..17683)
                     /gene="gndA"
                     /locus_tag="OO150_000503"
     CDS             complement(16277..17683)
                     /gene="gndA"
                     /locus_tag="OO150_000503"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5514756.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIT
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFHDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELIAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDNAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(17788..18621)
                     /locus_tag="OO150_000504"
     CDS             complement(17788..18621)
                     /locus_tag="OO150_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5514757.1"
                     /translation="MNHRLEKFSVLISIYKNDLPQFFEVALRSIFHDQTLKPDQIVIV
                     ADGELHQTHIDIINSFIDDVGNKIVTFVPLPRNVGLANALNEGLKACRNELVARMDAD
                     DISLPHRFEKQISFMINNSEIDVCGSFIDEIETVTEEFISTRKVPLEHREIVKFARKR
                     SAVSHPSVIFRKNTVLAVGGYPPFRKSQDFALWSLLIVHNARFANLPDILLKMRTGRN
                     LMARRGLSYLLYEYKVLYYQYKIGFIRKNELISNAMLRTFFRIMPSKLKELMYSIVRN
                     R"
     gene            complement(18618..19487)
                     /locus_tag="OO150_000505"
     CDS             complement(18618..19487)
                     /locus_tag="OO150_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5514758.1"
                     /translation="MDTVYAVLVAYNPEHNDLKNAVELLLRQVTKVVVCNNSTNGYKY
                     AENSSGDVKIFNFNDNLGIAEAQSIGMKWAFENGADFILQMDQDSIPDPKMVEQLLTC
                     YKKLLKQNVNVGLVGSQDFDKVTGELNKARVKKGKPLTEVYYEVDSTLSSGSLIPKNS
                     WLIVGGMKDELFIDAVDHEYCWRLRAAGFKVIRNKNALLAHRLGDGRFKILNILSVGL
                     PSPFRHYYATRNIFLLLNKNYVPIYWKISSLVKLIGKVFLYPIFLPNGNKRLYFFLKG
                     INDGLMGRSGKMK"
     gene            complement(19504..20691)
                     /gene="wzy"
                     /locus_tag="OO150_000506"
     CDS             complement(19504..20691)
                     /gene="wzy"
                     /locus_tag="OO150_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen polymerase"
                     /protein_id="HCR5514759.1"
                     /translation="MTSDFYNSKDKSLSVLLFFGFIFFLTRSFPFIQYSWIMEGFLCL
                     CIMSFTKKIANGIYHYPVILIFLLALFINFIYSYIKGNDIAIIIRFYIIILFILCAYF
                     CSYGTISIVKIFLYLMVLQAVIISIISIYMTKTYGIGDYSALRHYFLENDYGDVYTYG
                     SGFYRVQIKGNALIPFAFMLHIVIKDYFYYRFKNTITVILAIGTIVAGNFAYFVSICL
                     FFMYIILCSKSNSRYAKLRKIIFGVFLTVILPFFITYSIELIIMKSNGADSSLGVRWD
                     QFTVLINDLTESVSNFVIGSGLGNVIKIQTPIRDYSAYIYYELQSVYFLNQLGVILFT
                     LFLLINLLLTIKIIKYSELCVLYFLYVSYAITNPYILDSNHVAVIIVLVTLSNVLKKM
                     KAK"
     gene            complement(20747..21979)
                     /gene="wzx"
                     /locus_tag="OO150_000507"
     CDS             complement(20747..21979)
                     /gene="wzx"
                     /locus_tag="OO150_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen flippase"
                     /protein_id="HCR5514760.1"
                     /translation="MSIVKNTLWNISGYIIPSLIAIPALGILSRILGTEQFGLFTLAI
                     ALVGYASIFDAGLTRAVIREVSIYKNVHKELRAIISTSTVILTILGLIGGSVLFLSSN
                     VIVKLLNINANHVVESVKAIYIISATIPLYLLNQVWLGIFEGMEKFRKVNLIKSINNS
                     FVAGLPVIFCFFHGGLLSAIYGLVMARVLSLIVTFIFSRKLIISSGLSVKIVTVKRLI
                     GFGSWITVSNIISPIMTYMDRFILSHIVGADKVSFYTAPSEGIQRLTILPSALSRAIF
                     PRLSSELQSVKQTKILSYFIMVIGILPIVMLIIILSDFIMSAWMGPTYHGTPGIVLKI
                     LAIGFFFNCIAQIPFVSVQASGRSKITAIIHLLEVIPYLCILYIFIYHWGIVGAAIAW
                     SVRTSLDFLILLLIDTKY"
     gene            complement(21976..22533)
                     /gene="rfbC"
                     /locus_tag="OO150_000508"
     CDS             complement(21976..22533)
                     /gene="rfbC"
                     /locus_tag="OO150_000508"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5514761.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPDSDRGIVWNDPILSIKWPTI
                     EHNNYILSIKDARAKELHNMKELFL"
     gene            complement(22538..23416)
                     /gene="rfbA"
                     /locus_tag="OO150_000509"
     CDS             complement(22538..23416)
                     /gene="rfbA"
                     /locus_tag="OO150_000509"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5514762.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(23474..24373)
                     /gene="rfbD"
                     /locus_tag="OO150_000510"
     CDS             complement(23474..24373)
                     /gene="rfbD"
                     /locus_tag="OO150_000510"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5514763.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKEANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAAKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELITTTAI"
     gene            complement(24373..25458)
                     /gene="rfbB"
                     /locus_tag="OO150_000511"
     CDS             complement(24373..25458)
                     /gene="rfbB"
                     /locus_tag="OO150_000511"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5514764.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTRFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTLEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            complement(25831..26724)
                     /gene="galF"
                     /locus_tag="OO150_000512"
     CDS             complement(25831..26724)
                     /gene="galF"
                     /locus_tag="OO150_000512"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5514765.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(26956..27951)
                     /locus_tag="OO150_000513"
     CDS             complement(26956..27951)
                     /locus_tag="OO150_000513"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HCR5514766.1"
                     /translation="MNNNVLLIGASGFVGTRLLETAIADFNIKNLDKRQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(28109..29503)
                     /gene="wcaM"
                     /locus_tag="OO150_000514"
     CDS             complement(28109..29503)
                     /gene="wcaM"
                     /locus_tag="OO150_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5514767.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGHTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMSNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLVYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(29514..30734)
                     /gene="wcaL"
                     /locus_tag="OO150_000515"
     CDS             complement(29514..30734)
                     /gene="wcaL"
                     /locus_tag="OO150_000515"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5514768.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEILALQKGDTQN
                     THAAWTKYNLAARTHWLQDEPTGKIAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLGTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(30731..32011)
                     /gene="wcaK"
                     /locus_tag="OO150_000516"
     CDS             complement(30731..32011)
                     /gene="wcaK"
                     /locus_tag="OO150_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5514769.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMMALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(32287..33765)
                     /gene="wzxC"
                     /locus_tag="OO150_000517"
     CDS             complement(32287..33765)
                     /gene="wzxC"
                     /locus_tag="OO150_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5514770.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCLAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPQGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKPLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQFLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(33767..35107)
                     /gene="wcaJ"
                     /locus_tag="OO150_000518"
     CDS             complement(33767..35107)
                     /gene="wcaJ"
                     /locus_tag="OO150_000518"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5514771.1"
                     /translation="MVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQML
                     GGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTS
                     IGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLMESFRNQPWLGFEVVGVYH
                     DPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGARVKKLVHQLADTTCSVLL
                     IPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRAEDIVLATLILLLISPVLC
                     CIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKV
                     GNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPCITG
                     WAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY"
     gene            complement(35212..36582)
                     /gene="cpsG"
                     /locus_tag="OO150_000519"
                     /gene_synonym="manB"
     CDS             complement(35212..36582)
                     /gene="cpsG"
                     /locus_tag="OO150_000519"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5514772.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTSVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSRDALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(36775..38211)
                     /gene="cpsB"
                     /locus_tag="OO150_000520"
     CDS             complement(36775..38211)
                     /gene="cpsB"
                     /locus_tag="OO150_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5514773.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSSVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(38214..39437)
                     /gene="wcaI"
                     /locus_tag="OO150_000521"
                     /pseudo
     CDS             complement(38214..39437)
                     /gene="wcaI"
                     /locus_tag="OO150_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(39434..39913)
                     /gene="gmm"
                     /locus_tag="OO150_000522"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(39434..39913)
                     /gene="gmm"
                     /locus_tag="OO150_000522"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5514774.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLPGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(39916..40881)
                     /gene="fcl"
                     /locus_tag="OO150_000523"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(39916..40881)
                     /gene="fcl"
                     /locus_tag="OO150_000523"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5514775.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQSTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(40884..42005)
                     /gene="gmd"
                     /locus_tag="OO150_000524"
     CDS             complement(40884..42005)
                     /gene="gmd"
                     /locus_tag="OO150_000524"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5514776.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYCESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIVTGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLDDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
BASE COUNT        11010 a        10668 c         9704 g        10648 t
ORIGIN      
        1 cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg
       61 gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg
      121 ttatcaccag caaaacggca accaacggcc acacagacaa attaaaagtc tcagcaatag
      181 agttatgttc agtactgtag tgctgcaagg tttgatttaa accactggca tgtggagaac
      241 gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct
      301 cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga
      361 agatataccc acctataaca gtaccaggcc acgggaacgc cccggcttcg taagtcagta
      421 ggcccgccta aattaacgca aaaaccccca cactttgtac cgaaattgca atcagcaagg
      481 cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa
      541 aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca
      601 ttgaaaacat aaattaatta accagatgaa tgttaatgag gaaattattc atgactggtg
      661 gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttcttaa tgaggatttc
      721 gataaaaaag aataaaaaaa ccggagccaa atattcattc gactccggtt gattattaga
      781 aaagatgatt atgctacgtc ttcgtactgc ggaaccgggt tacggaagct cttggtgacg
      841 caagccaggt aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct
      901 tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac
      961 tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg cgagatgacc
     1021 gacaggttaa cgaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt
     1081 accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc
     1141 ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac gccgtcacga
     1201 cccattacgt acatcagacg cgctacgcct gcatgcgccg ccataccgga cgccagtacg
     1261 gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg
     1321 atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc
     1381 aggaagtaag ttgcaaagat gaagatcatg ccgccgatca gcgcggtcag gaaaatcgca
     1441 cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat gccgtcaaag
     1501 ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg
     1561 ccagaccaga atggacgagt gctcgccagc gtaccagcgc cttcaccttc aaatacgcga
     1621 taaacaacca tgcccagaat caccgcgatc agcaccacct gcaacacgac gattacagtg
     1681 ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcatgaa ggccaccaac
     1741 gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata gattttcgcc
     1801 agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata
     1861 aagccgacag tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga
     1921 tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac
     1981 gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa agtatcaaac
     2041 agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt
     2101 ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt cattctcctt
     2161 tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac
     2221 aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa
     2281 tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt agtcattttt
     2341 tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca
     2401 taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga
     2461 tcgcactatt tgctaaaatt tcatcccgcc accagccact ggcaagtcct gcggtctgtt
     2521 cattccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc ttctcgatta
     2581 acgcgccact atcgagaaaa tgttgcgcca gataacgggg taaatagccg cagccgaggc
     2641 cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt
     2701 cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc
     2761 gatatcgctt aataatacga cgatttaacg gctcttcttc cagggctaac ggatgatgag
     2821 gcgcgaccgc aaagacttgt tccagatcgc ccaaccgtga aaaaccaaac tcactggaag
     2881 atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc agggcatccc
     2941 atgaaccacc cagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa
     3001 aagcttcaat cagtggcgct agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca
     3061 gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttcc cgcacggtat
     3121 gcagaacttc ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt
     3181 gaccgctacg atcaagcaat tgaatattaa ggtcgctttc cagcttgtga acggtgtaac
     3241 tcagagcgga cggcgtcttg tagagtttgg ccgacgccgc cgcaaaactg ccttcttttt
     3301 ctaaggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt
     3361 gtatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc ggatcgggat
     3421 actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc
     3481 cgctgtccag actatttctg aactgcggag gttccagccc cagtaaacgg gccatctgcg
     3541 gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt
     3601 taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa
     3661 caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga tgacgccccg
     3721 gtccgaccag gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc
     3781 agtcttcgag ttcttctaat actagtccac tgttggttac cggattacgc ggggtagttt
     3841 ctttcaccat gccttgcgca tcgccataga cagatgttga gctggtaaaa ataattcggg
     3901 gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat
     3961 cgccggagcc gctacgacgt gccggaagcg taatgaccag tgcatcggca tccatcaggg
     4021 catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa
     4081 tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc
     4141 agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac ccgacaattg
     4201 cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta
     4261 tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca
     4321 atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg
     4381 gactagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc ttcaaatggc
     4441 gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc
     4501 tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac tcaataatgt
     4561 ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt
     4621 tagcggggga acgcagtaga taagccgtct cttccagcga gttgtattct tcgagtgaca
     4681 tcagaacaca agcctctcca ttctgacgag taataaggat cggggcatga tcttcaacgg
     4741 ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt
     4801 caatctcctc ttttgtacag ttcattgtac aatgatgggc gttaattaac tatttattaa
     4861 ttagtttgta gatcaaggta ttgtcagtga gacgaaaatc caggcttcgc tatttttggt
     4921 gccgtcagct tagaggacag tcctcttagc cccctccttt ccccgctcat tcattaaaca
     4981 aatccatcgc catagaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg
     5041 ttaaaagaca tcagttgaat aaacattcac agagactttt atgacacgcg ttcaatttaa
     5101 acaccaccat catcaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
     5161 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
     5221 gcttttttat tgcgcggttg ataacggttc agacaggttt aaagaggaat aagaaaatga
     5281 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
     5341 gcgaattgct ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
     5401 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgacgacatt cccggtctgg
     5461 taatggacgg cgtggttgac ctcgggatta tcggcgaaaa cgtgctggaa gaagagctgc
     5521 ttaaccgccg cgcccagggt gaagatccac gctactttac cctgcgtcgt ctggatttcg
     5581 gcggctgccg actttcactg gcaacgccgg ttgatgaagc ctgggatggc ccgctctcct
     5641 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     5701 aaggtatctc ttttaaatcc tgcttactga acggttctgt tgaagtcgcc ccgcgtgccg
     5761 gactggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     5821 tgcgcgaagt cgaagttatc tatcgctcga aagcctgcct gatacaacgc gatggcgaaa
     5881 tggaagaatc caaacagcaa ctgatcgaca aactgctgac ccgtattcag ggtgttatcc
     5941 aggcgcgtga atcaaaatac atcatgatgc acgcaccgac cgaacgtctg gatgaagtca
     6001 tcgccctgct gccaggtgcc gaacgcccaa ctattctacc gctggcgggt gatcaacagc
     6061 gcgtagcgat gcacatggtc agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     6121 aagcgctggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     6181 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     6241 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcactgtta acgatattct
     6301 cgataacgtg aaagcacgcg gcgatgaggc cctgcgggaa tacagcgcga agtttgataa
     6361 aaccacggtt accgcactga aggtgtctgc tgaggaaatt gccgccgcta gcgaacgcct
     6421 gagcgacgag ctaaaacagg cgatggcggt ggcagtgaag aatattgaaa ccttccacac
     6481 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcca ggcgtgcgtt gccagcaggt
     6541 cacgcgcccg gtcgcttcag ttgggttgta tattcctggc ggctccgccc cgctcttctc
     6601 aacggtatta atgctggcga ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     6661 ctcaccgccg ccgattgccg atgagatcct ttatgcggcg cagctgtgcg gtgtgcagga
     6721 cgtgtttaac gtcggcggcg cacaggccat tgccgcactg gcgtttggta cggaatctgt
     6781 gccgaaagtg gacaaaatct tcgggccggg caacgccttt gtcaccgaag cgaaacgtca
     6841 ggtgagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgtt
     6901 ggtcattgcc gacagtggcg caacgccgga tttcgtggct tctgatttgc tttctcaggc
     6961 tgaacacggc ccggactcac aggtgatttt actgacgccc gctgctgata tggcgcgtcg
     7021 cgttgccgag gctgtcgaac gccagctggc agaactgccg cgtgctgaaa ccgcccgtca
     7081 ggcactgaac gccagccgcc tgatcgtgac tgaagattta gcgcagtgcg tagagatctc
     7141 caaccagtac ggcccggagc acctgatcat ccagacccgc aacgcccgcg atctggtcga
     7201 tggcattacc agtgccggtt cggtatttct tggtgactgg tcactggaat cggcaggtga
     7261 ttacgcctcc ggcaccaacc acgttctgcc gacttacggt tacaccgcca cctgttccag
     7321 cctcgggctg gcagatttcc agaagcgcat gaccgtgcag gaactgtcga aagaaggttt
     7381 ctccgcgctg gcttcgacca ttgaaacatt ggccgccgcc gagcgcctga ccgcccataa
     7441 aaatgccgtt actttacgtg ttaacgccct taaggagcaa gcatgagcat cgtgactatt
     7501 accgatttag cgcgtgaaaa cgtgcgcaac ctgacgccgt atcagtcggc gcgtcgtctg
     7561 ggcggtaacg gcgatgtctg gctgaacgcc aacgaatatc ccacagccgt ggaatttcag
     7621 cttactcagc aaacgctcaa ccgctacccg gaatgtcagc cgaaagcggt gattgaaaat
     7681 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     7741 ggtattgaac tactgattcg cgctttttgc gaaccgggta aagacgccat cctctactgc
     7801 ccgccaacgt acggcatgta cggcgtcagc gccgaaacca ttggcgtcga gtgccgcaca
     7861 gtgccgacgc tggacaactg gcaactggat ttacagggca tttccgacaa gctggaaggc
     7921 gtaaaagtgg tctatgtttg cagccccaac aacccgaccg ggcaactgat caatccgcag
     7981 gattttcgca ctctgctgga gttaacccgc ggtaaagcga ttgtggttgc cgatgaagcc
     8041 tatatcgagt tttgcccgca ggcatcgctg gctggctggc tggcggaata tccgcacctg
     8101 gctattttgc gcacactgtc gaaagctttt gctctggcgg gccttcgttg cggatttacg
     8161 ctggcaaacg aagaagtcat caacctgctg atgaaagtga ttgcccccta cccgctctcg
     8221 acgccggttg ccgacattgc ggcccaggcg ttaagcccgc aggggatcgt cgccatgcgt
     8281 gagcgggtgg cgcaaattat tgcagaacgc gaatgcctga ttgccgcact gaaagagatc
     8341 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     8401 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     8461 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     8521 gtcattgacg ccttacgtgc ggagcaagtt taatgagtca gaagtatctt tttatcgatc
     8581 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggatcgt tttgacaaac
     8641 tcgcctttga accgggcgtg atcccggaac tgctgaagct gcaaaaagcg ggctacaaac
     8701 tggtgatgat cactaatcag gatggtcttg gaacacaaag tttcccgcag gcggattttg
     8761 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     8821 tgctgatttg cccgcacctg cccgccgatg aatgcgactg ccgtaagccg aaagtaaaac
     8881 tggtggagcg ttatctcgct gagcaagcga tagatcgcgc caacagttat gtgattggcg
     8941 atcgcgcgac cgatattcaa ctagcagaaa acatgggcat tactggttta cgctacgacc
     9001 gcgaaaccct gaactggccg atgattggcg agcaacttac caaacgagac cgttacgccc
     9061 atgtagtgcg caacaccaaa gagacgcaga ttgacgttca ggtgtggctg gatcgcgaag
     9121 gtggcagcaa gattaacacc ggcgttggct tctttgatca tatgctggat cagatcgcca
     9181 cccacggcgg tttccgcatg gaaatcaacg tcaaaggcga cctctatatc gacgatcacc
     9241 acaccgtcga agataccggc ctggcgctgg gtgaagcgtt aaaaattgcc ctcggcgata
     9301 aacgcggtat ttgtcgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     9361 cactggatat ctctggtcgc ccgcacctgg aatataaagc tgaatttacc taccagcgcg
     9421 tgggcgatct cagcaccgag atgatcgagc acttcttccg ttcgctctct tacaccatgg
     9481 gcgtgaccct gcacctgaaa accaaaggta aaaacgatca ccaccgtgta gagagcctgt
     9541 tcaaagcttt tggtcgcacc ctgcgccagg ccatccgcgt ggaaggcgat accctgccct
     9601 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     9661 ctcggtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     9721 cgtcgtgttg ctggccgata aactattttt acccggcgtt ggcaccgcac aagcagcgat
     9781 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     9841 aggcatctgc ttagggatgc aactgctggg gcggcgcagc gaagagagca acggcgtcga
     9901 tttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc taccgctgcc
     9961 ccatatgggc tggaaccgcg tttacccgca ggcaggcaac cgcctgtttc aggggattga
    10021 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
    10081 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
    10141 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
    10201 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
    10261 cagggcgatt acggcaaaca gcgcgattac ggtaacgacc cgctgccgcg cttacaggat
    10321 tacgccacgc agggtgccga agtgttgcat ctggtggatc tgaccggggc aaaagatcca
    10381 gcaaaacgtc aaatcccgtt gattaaaacc ctggtcgcgg gcgttaacgt tccggtgcag
    10441 gttggcggcg gcgtgcgtag cgaagaagac gtggcggcgt tactggaagc gggcgttgcg
    10501 cgcgtggtgg tcggctccac cgcggtgaaa tcaccagaaa tggtgaaagg ctggtttgaa
    10561 cgtttcggtg ccgatgcctt agtgctggcg ctggatgtcc gtattgacga gcaaggcaac
    10621 aagcaggtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
    10681 gaaacctatc tgcccgtcgg cctgaaacat gtgctgtgta ccgatatctc gcgcgacggc
    10741 acgctggcag gttctaacgt ctctttatat gaagaagtgt gcgccagata tccgcaggtg
    10801 gcatttcagt catccggcgg cattggcgac attaacgatg ttgctgccct gcgtggcact
    10861 ggcgtgcgcg gcgtaatagt tggtcgggca ttattggaag gtaaattcac cgtgaaggag
    10921 gccatcgcat gctggcaaaa cgcataattc cgtgtctcga cgttcgtgat ggtcaggtgg
    10981 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgccg ctggcaaaac
    11041 gctacgccga agaaggtgca gacgaactgg tgttctacga tatcaccgct tccagcgatg
    11101 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgttct
    11161 gcgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
    11221 ataaaatttc catcaactcg cctgcgctgg cggatccgac gttaattact cgcctggcgg
    11281 atcgttttgg cgtgcagtgt attgtggtgg gtattgatac ctggtacgac gctgaaaccg
    11341 gtaaatatca tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc actcagtggg
    11401 aaacgctcga ctgggtacag gaagtgcaaa aacgcggtgc cggagaagtc gtcctcaaca
    11461 tgatgaatca ggacggcgtg cgtaacggtt acgacctcga acaactgaaa aaagtgcgtg
    11521 aagtttgcca cgtcccgcta attgcctccg gtggcgcggg caccatggaa cacttcctcg
    11581 aagccttccg tgatgccgac gttgacggcg cgctggcagc ttccgtattc cacaaacaaa
    11641 taatcaatat tggtgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
    11701 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggac ttatgccggt
    11761 gattgtgcaa cacgcggtat ccggcgaagt gttaatgctg ggctatatga acccggaagc
    11821 cttagacaaa accatagaaa gcggcaaagt cgccttcttc tcgcgcacta aacagcgact
    11881 gtggaccaaa ggcgaaacgt cgggcaattt cctcaatgtg gtgagtattg caccggactg
    11941 cgacaacgac acgttactgt tactggtgaa tcctatcgga ccaacctgcc ataaaggcac
    12001 cagcagctgc ttcggcgaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
    12061 gctcgccgaa cgcaaatctg ccgacccgga aacctcctac accgccaaac tgtatgccag
    12121 cggcaccaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
    12181 gacagtacat gaccgctttg agctcaccaa cgaggcgtct gatttgatgt atcacctgct
    12241 ggtgctgttg caggatcagg atctggactt aacgacggtg attgagaacc tgcgtaaacg
    12301 gcatcagtga gttgcggggt aagcggatgc gatattgttg tagcatccgg aaaaaaaacg
    12361 ggcaaggtgt caccaccctg ccccttttct ttaaaaccga aaagattact tcgcgttgta
    12421 attacgcaga gcgttacgcc ccaagacaat ccccgcgcca accatgccgc ccagcagcac
    12481 cgccagaatc aaggtaattg cctttttcgg gctatcgcga cgaataggta acgtcggttt
    12541 cattacatag cggtaagcat gaatatcaag tttatcaacg tcaagattgt cgatatccag
    12601 caggttttga cgagtctgat agtagtttgg tgagaacacc aacggacgag tcgcttcatg
    12661 cttaatcatc gactccagcg cttcgctccc caaaaggaac aacgtatctt gtgtcacatc
    12721 ttcagtctgc tgaacctgtg gctttgtcac ctgcgcctga ttcgcatact gcaacgcttc
    12781 ctgaatctga cggatacgca gatctttctg ctcctgcgca actacttcct gagttttcag
    12841 agaatcctgc aagtttttca tacgcagcgc gatgttgtct ttcaggtctt ttcccagttc
    12901 ttcatttacc tggtcgtcaa cttgctggat atacttcgcc agctgcttct gagcaccttc
    12961 aggtgtttca ccaacataag acaccgctaa aggtaatgac tgatttttga cggtaggctc
    13021 aatggtcagc ttttctgctt cttcctggtt atccagcgtt tccgctaatg ctgagaatgc
    13081 agtgctgtaa cgtccgataa gtgaagcctg aatgtcggag actttcggta cagccgatcc
    13141 gtagatcaca ttcatcgcgt tggtataacc agcgatttgc cccacatcag gttgagtaac
    13201 gatagctgtg gaagtccact tttctttggc aacggccaga taccctatag ccagggcaat
    13261 agcaacaatg acagagatga taattgtcat cttgccacgc cacaactgca ccagtaaatc
    13321 aatcaaatca atctgttccg gatcatggtt ttgcccagaa acattattat tttctactct
    13381 catcattacc ctaacggacg aaataatttt aagtcgccat agtttagcga taatcgccat
    13441 gaatgctata ggataaatga taaaaataat gaattattac aaagggaaca taagagaatc
    13501 agggcgaaaa tcgccatgat aacaggatgt tagtcactgc caaagagatc gcgggtgtag
    13561 actttgtctg ccacatcctt aagctcttct gccatacggt tggagataat gacgtcggct
    13621 tgtatagtca gcttagaaca gctttgattg taaaatgagc acgttaccag aaaaaacagc
    13681 caagtttaga actgataccg attatctttt tttcttgtct aacggtataa tttaactttc
    13741 agttatgcca gatgaagatt ggctatattc tagcctgagc gaggattata aatgaaattt
    13801 ctggttacgg gagctgctgg ctttatcggt ttctatctaa gtaaacggct tcttgcagct
    13861 ggtcatcagg ttgtaggcat tgacaactta aatgattatt acgatgtcaa cctcaaacaa
    13921 gcacgacttg atttactcaa gcacgacaac ttcagttttt ataaaattga cctggccgat
    13981 cgtgagaaaa tggcggcact gtttgcagac gagcggttcg aacgcgtaat aaacctcgct
    14041 gcgcaagccg gtgtacgtta ctctcttgaa aatcccaatg catatgcaga tgcaaacctg
    14101 attggattcc tgaacatact agaaggatgt cgccataata atgttcagca tctactttac
    14161 gcttcctcca gttctgttta tggcatgaac cgcaagatgc ctttctctac agatgactct
    14221 gtagatcatc ctgtttcgct ttatgcagca actaaaaaag cgaatgaact catggcccat
    14281 acatattctc atttgtatgg cttaccgacc acagggctgc gtttctttac ggtttatggt
    14341 ccatggggac gtccggatat ggcattattt aaattcacta aagccatgct ggaaggtaaa
    14401 agcattgatg tttacaactt cggcaaaatg aagcgtgact ttacttacat tgatgatatt
    14461 gccgaagcta ttattcgctt acaggatgtt attccagaaa aaaacccaca gtgggctgta
    14521 gaaacaggct cgcctgcaac aagttcagca ccatatcgtg tctataacat tggtaatagt
    14581 tcgcctgtgg agttgatgga ctatatcaat gcgttagaag aggctctggg tattgaagcc
    14641 aacaaaaata tgatgcctct ccaacccggt gatgtactgg aaaccagtgc tgatacaaaa
    14701 gcactgtatg acgtaatagg attcaaacct gaaacgtcag ttaaagaagg ggtaaagaac
    14761 tttgtagaat ggtatcgtaa cttctataaa gtttgatttt acaaaaccat aagaaaaggc
    14821 cctaatttat tagggccttt tcttagaatg aaacaaaata attaatcatt gccaaacaag
    14881 tcgcgcgtat aaactttatc tgctacatca gccagatcgg cagacatacg gttagaaata
    14941 ataacatcag cttcttgttt gaacgcatcc agatcacgta ccacgcgcga ccggaaaaaa
    15001 tcgtcctctt tcatagctgg ctcataaacg attacaggca cacctttcgc cttgattcgc
    15061 ttcataatac cctgaatcga ggaagcacga aaattgtctg aaccattctt cataatcaaa
    15121 cgatagacgc caacaacttt cggtttacgt gcaaggatag aatcggcaat aaaatctttg
    15181 cgcgtgcggt tggcgtcaac aattgccgag atcaggttat tcggcacaga ctggtaattt
    15241 gccagtaact gcttagtatc tttcggcaga caataaccac cataaccgaa tgacgggttg
    15301 ttgtagtgat taccgatacg cgggtcaagg catacgccct caataatctg gcgtgaatta
    15361 agtcccaggc tttcagcata actatcaagt tcattgaaat acgctacacg catcgccaga
    15421 taagtgttcg caaaaagttt aatcgcctca gcctcggttg agtcagtaaa caatgttggt
    15481 atgtcttgct taatggcgcc ttcctgtaat aacgcagcaa aacgtttagc gcgttcagac
    15541 tgctcgccaa tcacaatgcg tgatgggtgt aagttatcat aaagtgcttt accttcacgc
    15601 aaaaactcag gcgaaaagat cacattttca ataccaaaac gttctttaat ggactctgta
    15661 aaaccaacag ggatagttga ttttataatc attaccgcgt tgggattaat ttctgtcaca
    15721 tcacgaatga ccgcttccac gcttgaggta ttaaaataat ttgttttcgg atcataatcg
    15781 gtaggtgtgg caataataac gtaatcggca tttttatacg cgtcatactt atctgtcgta
    15841 gcgcggaaat tgagatcttt agtcgccaga tactcttcaa tctccttatc aacaagcggt
    15901 gactgcctct tgttaagcat gtccactttg gcctgaacga tatccagtgc aaccacttcg
    15961 tggttttgcg caatcagaat accatttgaa agaccaacat aacctgttcc tgaaattgtt
    16021 attttcatta gctctgactt cttccggtta aacatttaga gtggtcatta atccaccacc
    16081 acagcttcat ctactgcggg atttttaacg ctgatgtaga tgaactgtca aggcagcgat
    16141 cctgctgtgc ggcgctgtat tatatcgcgt ttttaactat aaattataaa aaaaggccct
    16201 aacctgccgc tttgtataat aaaaaagccc ggagggtttc tccgggcctt gctttgatta
    16261 attgatttaa atcagattaa tccagccatt cggtatggaa cacaccttct ttatcaatgc
    16321 gcttataagt atgcgcaccg aaatagtcac gctgtgcctg gatcaggttc gcaggcagaa
    16381 cagcggcgcg gtagctgtcg taataggcaa ccgcagcggc gaaggtcggc accgggatac
    16441 cgttctgtac tgcgtaagcg acgacatcgc gcagcgcctg ctggtagtca tcggcaattt
    16501 gcttgaagta aggagccagc aacaggttag cgatctgcgg attttcggca taagcatcgg
    16561 tgattttctg caggaactgc gcacggatga tgcagccagc acggaaaatc ttcgcgattt
    16621 caccgtagtt cagatcccag ttgtactctt cagacgcagc gcgtagctga gagaagccct
    16681 gagcgtaaga aacgattttg cccagataca gcgcacggcg aactttttcg atgaactcag
    16741 cattgtcgcc agctggctgc gcttgcgggc cagagagaac tttagatgcg gcaacacgct
    16801 gctctttcag agaagagata taacgtgcaa acacagactc ggtaatcagc gacagcggtt
    16861 cgccgagatc cagcgcgctc tggctggtcc atttgcccgt acctttgttt gctgcttcat
    16921 ccagaatcac atcaaccagg tagttaccct cttcatcttt tttggtgaag atatctttgg
    16981 tgatgtcgat caggtagctg ctcagttcac cgttattcca ctcggtaaag gtctgcgcca
    17041 gttcttcgtt ggtgaggttc aagccacctt taagcagaga ataggcttca gcaatcagct
    17101 gcatatcacc gtattcaata ccgttgtgaa ccatcttcac ataatgacct gcaccatcgg
    17161 caccaatata ggtaacgcac ggttcgccgt cttcagccac agcggcgatt ttggtcagga
    17221 tcggcgcaat cagttcataa gcttctttct gcccaccagg cataatggaa ggacctttca
    17281 gcgcaccttc ttcaccaccg gaaacaccgg taccgataaa gttaaagcct tctgcagaaa
    17341 gctcacggtt acgacgaatg gtgtcatgga agaaggtgtt accaccatca atgatgatgt
    17401 cacctttatc gaggtatggc ttgagggaat caatagcagc atccgtgcca gcacctgctt
    17461 tcaccattaa caggatgcga cgaggcgttt ccagagattc aacaaattct ttcaccgtat
    17521 agtaaggaac cagtttcttg cctggatttt cggtaatcac ttcttcggtc ttttcacggg
    17581 aacggttgaa aatagagacg gtataaccac ggctttcgat attgagcgca aggttgcgcc
    17641 ccatcactgc cataccgacg acgccgatct gttgctttga cattgtttac tcctgtcagg
    17701 ataccgctgg gtggtatgcg ggttatgctt aattatagaa tatgcctaat aaaaataaat
    17761 ccataacact taatcagaaa attattatta tcgattccta acgattgaat acatcagctc
    17821 ctttaattta gatggcatta tacgaaaaaa tgttctcaac atagcattac ttattaattc
    17881 attttttcga ataaaaccaa ttttatattg ataatacaat actttatact cgtacaataa
    17941 atatgacaat ccacgtcgag ccataagatt acgaccagtt cgcattttta ataaaatatc
    18001 tggaagattt gcaaatcttg cattatgtac aattaatagg ctccacaatg caaaatcttg
    18061 agattttctg aatggaggat aaccaccaac agctaatact gtattctttc taaaaattac
    18121 agaaggatgg ctaactgcgc ttcgtttcct cgcgaattta actatttctc tatgttcgag
    18181 aggcactttg cgtgttgaaa taaactcctc agtaacagtt tcaatttcat caataaaact
    18241 gccacataca tctatttctg aattattaat cataaaagaa atttgtttct caaaccgatg
    18301 aggcaaagaa atatcatcag catccattct tgccactaac tcattcctac aagcctttaa
    18361 tccttcattt aaggcattag ccaatccaac atttctaggt aaaggtacaa atgttactat
    18421 tttattgcca acatcatcaa tgaatgaatt tataatatcg atgtgtgttt gatggagttc
    18481 tccatctgca acaattacta tttgatctgg cttaagtgtt tgatcgtgaa aaatagagcg
    18541 tagagccacc tcaaaaaatt gcggtagatc atttttataa atgctaatta aaactgagaa
    18601 tttttctaat ctatgattca tttcatttta ccacttcgac ccattaaacc gtcattaatg
    18661 ccttttaaaa aaaaatataa ccttttatta ccatttggaa ggaaaatagg atataaaaaa
    18721 acctttccaa ttaatttaac cagactagaa attttccagt agatgggtac ataattttta
    18781 tttaataaaa gaaagatatt tcgagtagca taataatgac gaaatgggct tggcaaaccg
    18841 acagaaagaa tatttaagat cttaaatcgc ccatctccaa gtctatgtgc aagtaacgca
    18901 tttttattcc taattacttt aaacccagca gctcttaatc tccaacaata ttcatggtct
    18961 accgcatcga taaaaagctc atctttcatt cctccaacaa tcaaccaact attttttggt
    19021 attagactgc cagaacttaa tgtactatct acctcataat aaacttctgt aagtggtttc
    19081 ccttttttta cccttgcttt atttaattca ccagttactt tatcaaaatc ttgtgaacca
    19141 actaaaccaa cattgacatt ttgtttaagc aattttttgt aacaagtaag taactgctct
    19201 accatcttag gatcaggaat actatcctga tccatttgca atataaaatc agcgccattt
    19261 tcaaaagccc atttcattcc tatactttgg gcttctgcta tgcctaaatt atcattgaaa
    19321 ttgaatattt ttacatcgcc tgaagaattt tcagcatatt tataaccatt tgtagagtta
    19381 ttgcaaacga caactttagt aacttgtctc aacaataatt caaccgcatt ttttaaatca
    19441 ttatgttctg ggttgtaagc aaccaaaacg gcatatacag tgtccatctt caccttaaaa
    19501 ccttcattta gctttcatct tttttagaac attacttaat gtcactaata caattattac
    19561 agcaacatgg ttagagtcta aaatataagg attagtaatt gcataagaaa catatagaaa
    19621 atatagcaca cacaactcac tgtattttat gattttaatc gtgagaagga gattaattaa
    19681 taaaaacaaa gtaaataaaa taacgccaag ttgatttaaa aaataaactg actgcaattc
    19741 ataatatata tatgcactat aatcacggat aggagtttga attttgatga cattacccaa
    19801 accagaacct ataacaaaat ttgatacaga ctctgtaaga tcattaatta atacagtaaa
    19861 ctgatcccat ctaactccta aagaagaatc agctccattt gatttcatga ttatcaactc
    19921 aattgaatat gtaataaaaa aagggagaat cacagtaaga aaaaccccaa aaataatttt
    19981 ccttaattta gcgtatcgtg agttagattt agaacatagt ataatataca taaaaaacaa
    20041 gcatatcgaa acaaaatatg caaaattacc agccactata gtacctatag ccagaataac
    20101 ggttattgta tttttgaatc gataatagaa ataatctttt atgactatat gcaacataaa
    20161 ggcaaatgga atgagagcat ttcctttaat ttgaactcta tagaaaccac ttccatatgt
    20221 ataaacatca ccataatcat tctccaaaaa ataatgtctt agtgctgaat aatcaccaat
    20281 accatatgtt tttgtcatat aaatactaat gatggatata ataaccgcct gtaataccat
    20341 taaatataaa aatattttaa caatcgagat ggttccataa gagcagaaat aagcacataa
    20401 tataaataat atgataatat aaaacctaat tattatcgct atatcgttac ccttgatata
    20461 ggaataaata aaatttataa aaagagctaa tagaaatatt aaaataacag gatagtgata
    20521 tattccgttt gcaattttct ttgtaaatga catgatacaa agacataaaa acccctccat
    20581 aatccaacta tattgaataa atggaaagct acgtgtaagg aaaaatataa acccaaaaaa
    20641 caaaagaaca cttaaacttt tgtcttttga gttataaaaa tcagaagtca tgtttgcact
    20701 ctaattagat gggcttgagg aagtaatccc taaaatcaat tcgctattaa tatttcgtat
    20761 caattaataa taatatcaaa aaatctaacg atgttcttac agaccatgct attgcggctc
    20821 caacaattcc ccaatgataa ataaaaatat ataatatgca taaatatggg ataacttcga
    20881 gcaaatgaat aatagctgta atttttgatc ttccactagc ctgaactgaa acaaatggga
    20941 tttgtgcaat gcaattaaaa aagaaaccta ttgcaagaat ttttaatact atacctggcg
    21001 tcccatgata tgtaggtccc atccaagcgg acattataaa atctgataaa ataattatca
    21061 acattacaat tggaagtata ccaataacca ttataaaata tgataatatt ttagtttgct
    21121 ttaccgattg caattctgaa cttaatcttg gaaaaatagc tctggacaac gcacttggta
    21181 atatcgttaa gcgttgtata ccttcagacg gagcagtata aaaagaaact ttatcagccc
    21241 ccacaatgtg tgaaagaata aaacgatcca tatatgtcat aatagggcta ataatattgc
    21301 taactgttat ccagcttcca aagccgatta atcttttaac tgttacaatt tttacagaca
    21361 gcccagatga tattattagt tttcgactaa atataaaggt cactataagt gataagactc
    21421 ttgccataac taaaccatat atagcactta gtaatcctcc atgaaaaaaa cagaaaatca
    21481 ctggtaatcc agccacaaaa gagttgttaa ttgattttat taaatttact tttctgaact
    21541 tttccatccc ctcaaaaatc cccaaccaga cttggtttaa caagtataag ggtatggtag
    21601 ctgaaataat atatattgct ttgacagatt ctacaacatg attcgcgtta atgtttaata
    21661 atttaacaat tacattgcta ctcaaaaata gtacactacc gccaatcaag cccaatatag
    21721 ttagaattac cgttgaagtt gaaatgatcg ctcttaattc tttatgaaca tttttatata
    21781 ttgatacttc tcttataaca gctctggtca atccagcatc aaaaatactt gcatatccaa
    21841 ctaaggcaat agctaacgta aaaaggccaa attgctcggt ccctagaatt ctagacagta
    21901 tacctaacgc aggaattgct attaatgatg gtataatata cccacttata ttccataaag
    21961 tattctttac aatactcaca aaaataattc cttcatgtta tgcaattctt tagcccttgc
    22021 atctttaatc gataaaatat aattattatg ttctatcgtc ggccatttta tgctcagaat
    22081 aggatcattc catacaatcc ctctatcact atcaggatga taatagttcg tcgttttata
    22141 taaaaattcc gcagtctcgc tcagcaccaa aaaaccatgt gcaaatccct cagggatcca
    22201 caattgccgc ttattctcag cagataaatt caccccaacc catttaccaa aggtaggcga
    22261 cgatttacga atatcaacag ctacatcaaa aacctcacca acaacgcaac gtaccagttt
    22321 cccttgcgca taaggttcta actgataatg cagcccgcgt aaaacacctt tactagactt
    22381 cgaatggtta tcctgaacaa attcaacctt acgtcctaca gcttcttcga aaactttctg
    22441 attaaagctt tccataaaga aaccacgctc atcaccaaaa actttcggct cgaaaattaa
    22501 cacatcagga atttctgttt taattacgtt cattttatta ataaccttta atcattttca
    22561 gcagatactg tccataagca ttttttttca gcggctccgc taatgctttc acctgttcag
    22621 catcaataaa ccctttacgg taagcaattt cttctgggca ggaaaccttt agtccctggc
    22681 gctcttcaat ggtggcaatg aagttgcttg cttcaataag actctgatgt gtccccgtat
    22741 ccagccatgc ataaccacgc cccatcatgg caacggataa acgcccctgt tccatataaa
    22801 tacggttaat atcggtaatt tccagttcac cacgggcaga aggcttaagg tttttcgcca
    22861 tttcgacaac gtcgttatca tagaaataaa gcccggttac cgcataatta ctttttggtt
    22921 gtagcggttt ttcttccagg cttattgccg taccgttttt atcaaactca acgacgccgt
    22981 agcgttcagg atcattaacg tgataggcaa ataccgttgc accactttct ttgttaacag
    23041 cgacatccat taacttcggc agatcatgac cgtagaagat attatcacca agaaccaaag
    23101 cacaatcatc accaccgata aactcttcac cgataataaa cgcctgcgca agcccatctg
    23161 gagtcggttg cactttgtac tgaagattta gcccccactg gctaccgtca cctagcagtt
    23221 gttgaaaacg aggagtatcc tgtggcgtac taataatcag aatatcgcga atacccgcca
    23281 acatcagtgt agagagcggg taatagatca tcggcttatc ataaataggt aatagctgtt
    23341 tactgacagc catagtcaca ggataaagac gtgtaccaga accacccgct aaaataatac
    23401 ctttacgcgt tttcatttca tcattccttt taattcatct tgctccacca tcacgaacaa
    23461 gatgcaaaaa ctattaaatt gctgtagtcg taattaattc gttgagcatt cgtttcacac
    23521 caacctgcca gtcaggcaag acaagcgcaa agttctgctg aaatttttct gtattaaggc
    23581 gagagttatg tggacgacga gctggtgtag gataggctgt tgttggtact gcgttgagct
    23641 tgttgagtgc aaggggaata cctgctttgc gcgcctcttc aaaaaccagc gcagcataat
    23701 cgtgccaggt tgtggtacca ctggctacca gatggtacaa acctgcgact tccggtttat
    23761 tcagtgccac acgaatagca tgtgccgtac aatcagccag cagctcagca cctgttggcg
    23821 caccaaattg atcatttatc acagccagtt cttcgcgctc ttttgccaga cgcaacatcg
    23881 ttttggcgaa gttatttcct ttagctgcgt atacccagct ggtacggaaa ataagatgct
    23941 tcgcgcaatg ttcctgtaac gctttttctc cggctaactt ggtttcaccg taaacattta
    24001 gcggtgcggt tgcatccgtc tccagccatg gcgtgtcgcc atttccaggg aatacgtagt
    24061 cagttgagta atgaattacc caagccccaa cttcattagc ctcttttgca attgattcaa
    24121 cactagtcgc attgagtaat tgtgcaaatt cgggttctga ctcagcctta tctactgcgg
    24181 tgtgagccgc agcattaaca ataacatcag gtcgaattct tttgactgtt tcagctacac
    24241 cttcaggatt actaaaatca ccacaataat cagtggagtg aacatcaaga gcaatcaaat
    24301 tacccaaagg tgccagagca cgctgtagtt cccaacctac ctgccctgtt ttgccgaaaa
    24361 ggaggatatt cattactggc ggccctcata gttctgttca atccacgatt gataagcacc
    24421 acttttcaca ttatcaaccc attttgtatt ggacaggtac cattccaatg tcttccgaat
    24481 cccgctctca aacgtttcct gcggtttcca gcccaattcg cggctaatct tctctgcatc
    24541 aatcgcataa cggcgatcgt gtcccgggcg atcggcaaca taagtaattt gctcgcggta
    24601 agatttctct ttcggtacaa tctcatccag caaatcacaa atagtgagca ctacatcgat
    24661 gtttttcttt tcgttgtgtc caccaatgtt ataagtttca cccgctttac cttcggttac
    24721 gacggtatat aacgcacgcg catgatcttc aacatacagc cagtcacgaa tttgatcccc
    24781 tttgccataa ataggtaatg ccttaccttc cagagcattc agaataacca atggaatcaa
    24841 tttttccggg aaatgataag gaccataatt attagagcaa ttagtcacaa tggttggtaa
    24901 accataggta cgtttccacg cgcggactaa atgatcgctg gatgcttttg aagcggaata
    24961 agggctgctt ggcgcgtaag ctgttgtctc tgtaaataag ggtaattctt ctgtattatt
    25021 tacctcgtca ggatgaggca aatcaccata gacttcgtca gtagaaatat gatgaaaacg
    25081 gaatctagtt ttcttgtcgc tatcaagagc agaccaataa ttgcgagcgg cttccaaaag
    25141 gacatatgta ccaacaatat tggtttcaat aaatgccgca ggacctgtaa ttgaacggtc
    25201 aacatggctt tcagcagcca ggtgcatcac tgcatctggc tgatgctgag caaaaatccg
    25261 tgccattgca gctgcatcgc aaatatccgc atgttcaaaa acatagcgtt cagaatcaga
    25321 aacatcagca agtgattcca ggtttccggc gtacgttaat ttatcgacat taacaacact
    25381 atcctgcgta ttatttataa tgtgacgaac tacagcagaa ccaataaatc ctgcgccacc
    25441 agtaacaagt attttcacct aatttattcc atattgcttc agagcatgct gtgaaataag
    25501 cggctctcag tttgattaat agaagtatta atgcacgcta ccgcccctgg ctttacagct
    25561 accagagcac tgcatgcatg cctacgatgt gacgagcgtt acccactcgc gctaaacccg
    25621 aaaaattcaa aagctaattg tcttaccaat ccgctctgga aacaaggaaa atcctggaaa
    25681 actttgacta aaatcctatt gctaactcgt tgttattctg attgtttata taaaacaacg
    25741 gcaggaatat tcgcaacaaa ttactttcac cacgaatctt cactgccgtt ataattttct
    25801 tatcaaccgt tacatccggt cagattttca ttattcgctt aacagcttct caataccttt
    25861 acggaacttc gccccttctt tcaggttgcg cagcccatac ttcacaaacg cctgcatata
    25921 gcccattttt ttaccgcagt cgtagctgtc gccggtcatc agcattgcat caacggactg
    25981 ttttttcgcc agctcggcaa tggcatcagt cagctgaata cgtccccatg caccaggctg
    26041 agtacgttca agttccggcc aaatatcggc agaaagcaca tagcgaccaa cggccatgat
    26101 gtctgagtcc agcgtctgcg gctgatccgg tttttcgata aattcaacaa tgcggctgac
    26161 tttaccttcg cgatccagcg gttctttggt ctggatgacg gagtattcag agaggtcacc
    26221 cggcatacgt tttgccagca cctggctacg gcccgtttca ttgaagcgcg caatcatggc
    26281 agcaaggttg tagcgtagcg ggtcggcgct ggcgtcgtcg atcacaacgt ctggcagcac
    26341 cacgacaaat ggattgtcac caatggcggg tcgtgcacac aaaatggagt gacctaaacc
    26401 taaaggttcg ccctgacgca cgttcataat agtcacgccc ggcgggcaga tagattgcac
    26461 ttccgccagt agttgacgct tcacgcgctg ctcaaggaga gattctaatt cataagaggt
    26521 gtcgaagtgg ttttcgaccg cgttcttgga cgcatgagtt accaggagga tttctttgat
    26581 ccctgcagcc acaatctcgt caacaatgta ctgaatcatt ggcttgtcga cgatcggtag
    26641 catctctttg ggtatcgcct tagtggcagg caacatatgc atcccaagac ccgctaccgg
    26701 tataactgct tttaaattcg tcattatttt cctacctcta aggggctgat agtgcgtaaa
    26761 ttattgtcat aggttagcca aacggtatgg ctatatacca agcataactt tgattaaacc
    26821 ttacgataac actacacacc atcagcatct gggttactcg gattactcgg aaatccacat
    26881 actgataatt taatcagtac ctctttccga ataatcgtag tccaacctgg tccttttttc
    26941 tctgactcgt ctgcattact cagaaacaaa cgttatgtcg tcttttttgg catggacgaa
    27001 ttcatactgc agagttcgat ccagaccttg cgacagcgta tacggtgcaa caaaacctga
    27061 agaatgcact ttcgttgcgt caaactgtgt tgttgcgcag aattttttca cgcgcacaga
    27121 gctgacagcg tattttttgc ccgtaatttt gctcaggata tcaaagcaat atccacccag
    27181 cattcctagt gggtaaggca agtgcataga agggatcttt ttgttcaggc tttgttcaac
    27241 ttcagcaacc aactggttca tgttcaggtc tggcttatca acatagttat aaacctcata
    27301 acctgcggca acattcttca gtttgtactt gataaactca acaatgtttc caacataagc
    27361 catggactta tagttagtcc ctgcgcccac catcataaac ttgccgccag cgatctgttt
    27421 cagcaagtta tagacgttac cgcggttgcg ttcaccgaag ataacggtag gacggatgat
    27481 ggttaatgaa cgttctgttg gtgctttgtt ataccattca cgcagcactt cctctgcctg
    27541 ccacttactt ttgccgtagt ggttgaaagg gtcgtgtgga tggttttcgt cagggttgtg
    27601 tttgttcaaa ccataaacag caacggaact ggtaaagatg atatttttaa cgccattttt
    27661 ttccatggcc gccagcacat tgcgggtacc ctgaacgttg acatcataat agagagaagt
    27721 agggctgacg tcatcgcggt gttccgctgc cagtagtaca acagtgtcaa aaccggctaa
    27781 cgcctggtcg agtgcctgtt gatcacgaac atcaccaatc tgtgtgattt ctggataaaa
    27841 gtggctctgc cgtttgtcca ggttcttgat attaaagtca gcaattgccg tttcaagtag
    27901 tcgggttcct acgaatccgg aagctcctat gagcaaaacg ttattgttca taaatcactt
    27961 tagtctggtt gttacgtaag aaacacaaga taaagatgag taccttccct gagtagtcaa
    28021 tgctgcccag ccccagcttt aacagttagt gtgaggatta taatctttta gaacattata
    28081 tccagtaagt ttatgaatgg tcgcaaatct actctctccg ttccggcaat ctaaagttaa
    28141 tcttttccac attaacaata tggtgattaa tcctgtcgat gtcgacggag ctttgccctc
    28201 tttcattcat cgcatgaaca tttgcaagtg acagcagtgt ttcttttttc gccataaaga
    28261 caccgcgtac gtctttgcgc atgtcgaagt tcatgctcaa tgctggtcca actgaggatt
    28321 cctgcatcac cttgatatta cgcataaaaa gatgttgcgg tttgttgtgt aactccagcg
    28381 aggcacgctt catctcaatg ttagtcagtg ccacaaagga gacagcattc ccggcggaga
    28441 tttggatgcc gcgcaattta taaacaagat gggtgttatc cagttgaata ttattcactc
    28501 ggaaattttg cggtatcgag agatatttgc ctttaattac cccatagccg attaacatcc
    28561 cggcgctatt aatcatttca atattatcaa tcacgaaatt gtcacaaccg taaatagcaa
    28621 ctgtcgcgtt atcaatgcct gctttcttac tgaaatccgg cgtgatattg cgggctttga
    28681 tattacggat aacaaaatgt ttgccatttt caacatggat caactgccga caatccgatc
    28741 ccgtgatatt cgccacgaca aagtttttca ctgcctggtc ttccgggtag ttattatcgt
    28801 aggtactccc cgcaaggcct atgccaattc cccagttgat tttgccatta gtacagttga
    28861 tgcgctcgat gacatggtcg gagatcaaaa tatcactgtc gttaattgcc acgttccatt
    28921 caatggcgtc gccctgtaag tcgctgaact tacaattggt gatgttggca ccgataatct
    28981 ggttatgaaa tccctggcgt aagatggcgt aattagcgtg gctgacagtg aggttatcga
    29041 tggtcaggtt gctcatgacc cgtttgtttt tgccgccgat ataaatctgc gttaccgggc
    29101 caaagccgct catcgccagc cctttgatgg tgcagtcaga accacgcaca tccaaggtga
    29161 tgttatgcat actgccgccc tcctcccctg tcacctggct gccgtcctgt aagacaaatc
    29221 gccctctgcc gttgccgcgc aagcttccaa gaatgtgtaa cgttttaccg ggagggataa
    29281 agatgccggt gttgatattg tcacaaacca atccggcagg cacgacgacc gtatgccctt
    29341 cgctgaaggc ttgtttaaat gaggcgatcc agtcgtgtgg gttgtagtcg ttaatgttaa
    29401 cgctttgtcg ggcgggaagt gcgcgagcga aaggggtatg gaggaaggca agcgccgagc
    29461 ttgccgtcag gaaggtgcgt cgggagagtg ttttaaatgg catgcgttct cctctataaa
    29521 gcttgcagca agctggcgag ttctcgattg atcacctgct ggttaaaatc gtgttcgact
    29581 ttttcgcgcg cacgtttgac aaccggagcc agttcgtcgg tgcccagttg gctaaacgcc
    29641 gccagtcgtt gcgccagtgc gcgagcatcg ttctcaggca ccagccagcc ggatttgtca
    29701 gcctccacca gttccggtat tccgctatgc agagtagaaa ccaccggaat accgaccgcc
    29761 atcgcttcca ttagcgccac cggaatgcct tccatatcac catccgcgcc cgttaccgat
    29821 ggcaacagaa agacatccgc gtcgtcgagc atcgctttca cttcgtggct cggtttaaag
    29881 cccggcatct ctaccacatc ttccagttga tattgttcga tgagggtacg caggcgtcgt
    29941 tcccacgggc caatgccgag gatgcgatag cgaaatgcca cgccctgctc tttcaactga
    30001 cggcaggctt caatcgccac atgcaggcct tttttctcgg ttaagcgtgc gacggaaatg
    30061 atttccagcg gcgttgcggg cgctttcacc ggacgcgggc taaagcgcgt catgtccacg
    30121 cccatacgcg atacggcgat tttttccctc gggcagccca ttttttgcag ccttccggcc
    30181 cacaaatcgc ttatcggtaa catcaggtcg ccacggcgaa acagttgttg atattcggga
    30241 gtgtaatggt tgagcacttc ccgactggaa atatcaatgc cgtggaaaat ggtggcaatt
    30301 ttgccgcgaa tgacacccag ttcccgtagt tttgctgcgg ttacccccgc cgggccaaaa
    30361 tgagcgatga acacatcggc atgaagcggt gttgcgacct ggccgcaaat ggcagacaaa
    30421 atcaggttcc gcgactcggc accatagcgt ttgaggttga gcgcctgcca ggtattttta
    30481 cgataaatgc cgcgtaacgt ctggctggcg cggtggcgca gtttcgctat tttgcccgta
    30541 ggttcgtcct gtaaccagtg ggttctggcg gcaaggttgt atttcgtcca tgccgcgtgg
    30601 gtgttttgtg tgtcgccttt ttgcagcgcg agaatctcta cctcaaatcc catatcaata
    30661 aacgcggtaa tttggttaag gacgaaggtt tctgacgaca gcgggaattt cagtaaaaag
    30721 aagccgacct tcatttcacc tccccgatgc gctcaagcac agactgcacc atctgcattc
    30781 ctgtctgacg ctcacgactg acggcttcac taagtcgcgc attcagtgcc ggaagctggc
    30841 ctaaggtatc cgcaaccatc gcttgcaggc tgccgtctaa taaatgacgg atatcaattg
    30901 ccatctccgg tagtcccagc tgctgcataa tcccggcgga tttatgttcg tagttgatgg
    30961 caattgccgg agtggcaaaa ttcatcgaga taatggcaga gtgcaggcgc gtaccgacgg
    31021 tgagttcaca ggcaccgaga attttgccca tttccagatc gttgagttca tccatcacca
    31081 catggtaacg ggcaggatcg ctgatgtgct ggcgaagatt gagcgccatc atgcggtcgt
    31141 ctttgttata gctgtcgata cccgtacagg tggagagtgc aatgacctga tagccttcgt
    31201 cgagaatgcg attgaccacc ccggcaaagg ctttttcata cgcttgttga gtggtgccga
    31261 ggcgtttgtc aaacggtgcc agttcgcgca gggtaattgc taccgttttc tgttgtgcgg
    31321 caacgtccag ccagtgttga acagcatagc tggcggtgaa gtcttcggtg tggtgatcga
    31381 ccagccacgc ggtatcgacg ccatgttcca cttttgcggt agtgatattg ctgcgtttca
    31441 tcagatcaag actgaccgat tcgcgcagga tcagcgcgtc acagtgacca aaaacgtagt
    31501 tcgccagttg gttaaattgc tcatcctgga aggggccgac gctgtgacca atcataaaca
    31561 gcggcttttt cgccataaac gtgcaaagcg catgttcaaa ctgcggcacg ccgtagagat
    31621 cgacaaaaaa cgatcctccg acctgaataa tggcgtcgta acctgagagc aggcgcacga
    31681 agtcggtgaa tccctgggcg atggcgatat tacgcagctt gccagtgtcg gtgacgcgtg
    31741 agagcaatac ctggtgttga tagcggcgac ggaggacttt tttaacgcgc ccgacaacgc
    31801 ccgccgcgct gttgtgttgt ttcatttgca ggaacagagg atcgcccatt accgggcggt
    31861 tgagtagcca ggaagaactg accggatagc ggctcatcac gtccacttcg gcttgtggat
    31921 tgaggatgtt gatggcatca agtaagccgc gcaagatggc gctgtcgcca cgattgccgc
    31981 aagtgtggtt gcccagaata agtaatttca ttttttcctc ataaatttga tgccaagtga
    32041 ggctgtgttt gttttgttcg ttgccgcttt ttgcctgatg cgacgctgac gcgtcttatc
    32101 aggcctacaa gacccgagca cagaaccgta ggccggataa ggcgttcacg ccgcatccgg
    32161 cattcagtgc ctgatgcgac gctgacgcgt cttatcaggc ctacaggtcc cgagcacaga
    32221 accgtaggat ggataaggcg ttttacgccg catccggcaa ccgttgtcgg aaccgaaaac
    32281 agcaactcac cccgcccgta aaagcatttt cattttttcg ctgcgacaaa actgacgctt
    32341 cacttccacc accagcggat ggcgcgacag cacaatcatc accacaaacg ccagcacgcc
    32401 cgcggctatt tgcaccgcca gcagcatccc cagcgccagt tgccctttca gcacaatgcc
    32461 cagcgcataa ctgaccacca gcgtcggcaa cgagagataa aacggcagcc acaaactcag
    32521 gatgtactgg cgataactgg aaccaagaac cggtttaatc atcacgaagt aactcagaat
    32581 ggtgttgata atctgcacca gcaggaaacc gagcgtgacg ccgattgcgc ccgccatctg
    32641 cccaccaata acaatcgccg gaataaacag aaatgttttg aatacgttga atttaaagct
    32701 gatatcgacc cgcgctttcg ccatcagcag cgaaccaatc gggttaccca cggagcgcag
    32761 tagccccacc acacacagaa attgcagcac cggaataatg ctgttccact tctcaccaaa
    32821 gaccagtggt acaaagttat tcgacaccac cattagcccg agcagcgccg gaaagttgat
    32881 aatccccacc accgacagcg gtttgtagaa gttaatacgc agcttttcgg tgtcgtcctg
    32941 aattttggca aacgccggaa acaacacacg ggtgatgatt gggttcagct tcatcggcgg
    33001 cacaacggcc acgttgtaag ccaggttgta gccccctgcc acgcccgcgc cgagaatacg
    33061 cgccagcacc agcgttgaaa ggttggtatt gagatagttg atgatgctgt ccgccgtcag
    33121 ccaggcacca aagcgtaagt tcggtgccac cgacgccagc gagaaatgca gaccggggcg
    33181 ataaattttg cggccaaagt agccaaacag cagcgttctc accgcactat tgaccagata
    33241 accgaggatc gcggtcatcg ccagcggcca gaaatgggcg ctaaccaccg taaaagtgaa
    33301 gcccgccagt accgcgctgg tttcgatcat gccgattttg ttgaactcca gctccttttg
    33361 catcaacgcg cggaactgct gcccctgggg gatcaccaca aacgccagcg acaatgtttt
    33421 aatcaacggt gccaggtccg ggttattcag cacgtcgccg atgagatcac tcaacaaaaa
    33481 caccgccaga cacaccacaa gccccagccc gacgttcagc cagtacagcg tggtgagttc
    33541 aaggtggctg atttcttttc gctgaataat cgagttagcg ataccgaagt cagaaagcgt
    33601 atctgccagc gcgatgatca ccagcgacac agtaagcaaa ccgaactggt ggttgtcgat
    33661 aatccgcgcc agcacggtca tctgcaccag cccgaggccg atgatgatca ccgtggcaat
    33721 cgccgaccac ttcgcgccgc tgatggtttt ttcacgtaag ctcatatcaa tatgccgctt
    33781 tgttaacgaa acctttgaac accgtcagga aaacgatttt gatatcgaac cagacgctcc
    33841 attcacggat gtactcaagg tcgaactcga cgcgtttttc cattttctcc agcgtgtcgg
    33901 tttcgccgcg ccagccgtta atctgcgccc agccggtaat gcacggtttc actttatggc
    33961 gcagcatgta gccttcaatg agctggcgat actgttcgtt atgcgctact gcgtgcggac
    34021 gtggaccgac aatcgacatc cccccggtca gcacattgat aaactgcggt aattcatcca
    34081 gcgaggtgcg gcgcagaaag ttccccactt tagtgacgcg cggatcgttc tgcgtcgcct
    34141 gggtcaccac tttgtcgttc tccatcactt tcatggaacg gaacttccac actttgatcg
    34201 gcttgccatc catgccgtag cgagtctggc ggaaaataac cggccctggt gaactgagtt
    34261 tcaccgccag cgcaatacag cacagcaccg gggagatcag cagcaagatc agcgtcgcca
    34321 gcacaatgtc ttccgcacgt tttagcaggc ggttaacccc ggaaagcggc gtgtcataca
    34381 gcggcaccac cggaacgccg ttcatctctt cgagacgtga atggagaata ttgaaggtaa
    34441 agacatcggg gatcagcagc accgaacagg tggtgtccgc cagttgatgg accagttttt
    34501 tcactcgcgc gccgtcgcac atttgcatcg cgatatagac gttatgaatc ttacccgctt
    34561 tagcatcctc gaccagctgt tgcagattgc ccgcccagtc gttagaaaca ccgcccggtt
    34621 tcgggtcgtg gtaaacgccc accacttcaa accctaacca cggctgatta cggaagctct
    34681 ccatcagcat ttgtccggcg gctaaatccc ccgccaccgc gaccatgcgc ttgttatagc
    34741 catgattacg cagccagccc gccccaatgc gaatacatga acggcaaacc accagtccga
    34801 tactggtcag cccataccac gccagccaga ttttcagttg cgtgtcgaaa tcattgttga
    34861 acgccaccag tccggcgctg aaaatcacgc ttaaggtcca gttttgtagc aacagggcaa
    34921 attctgtcgc tgcccgaaca ccgcgccatg agcgataaaa atcggtgatg ccgcccagca
    34981 tctggaacac caccagcgta atcaacgcca ccaacaggtg catgtagagg aatgacagtc
    35041 cgctcacttc gcaaaccagc catagtccgg caaacatgat ggtgatatct gaaaagcgtt
    35101 gcaccataga gattaacgat gcattggttt tcgctcgcgc ttttttagat ttgtcatcgt
    35161 tgttcctgtt attagcccct tacccgcagt ggggtaaggg aagatccgac attactcgtt
    35221 cagcaacgtc agcagagttc gcgttcgcgc ttccatcagc ggcacatcac cgcgtgattc
    35281 cacattcaac cgcaccaccg gttcggtgtt ggaggagcgc agattaaagc gccagtcggc
    35341 aaaggtcatg ctgatgccat cggtgcgatc caccgccagc gcatcacggc taaaatgctg
    35401 ttccacgcgg ttaatcgcct caacgggttg cgccagtttg ctgttgatct caccgcttgc
    35461 cggaaacgcc gccatccggt cgcgcaccag ttcgcccagc gtttttcctt tcaggcacac
    35521 cagttcggcg accagcagcc acgggatcat gccgctgtcg cagtaagcga aatcacggaa
    35581 gtaatggtgg gcgctcattt cgccaccgta gatggcgtct tccttgcgca tacgttcttt
    35641 aataaaggcg tgtccggttt tcgacatcac cgacgtgccg ccagcggcag tcaccacatc
    35701 aacggtattc caggagagac gtggatcgtg gatgatcttc gcgccgggat ttttttcgag
    35761 gaacgcttct gccagcaggc cgacaatgta gtagccttcg ataaactgcc ctttttcgtc
    35821 aaacaggaag cagcggtcaa aatcgccatc aaaggcaatg cccatatccg cgccgtgttt
    35881 gatgaccgca ttgcgggtgt cgtcgcggca ttccggcagt agcgggttag gaataccgtt
    35941 ggggaaattg ccgtccggcg tgttgtgcac tttgattaat tccacgggtg cgccaagggc
    36001 tttaaagcgg gcttcaatgg cgtccaccac cggacccgct gcgccattcc cggagttaat
    36061 caccagcttg agcggcgtga ggtttttgac attgatataa ccgaacaggt gatcaacgta
    36121 agcgtcacgc aggttgattt gctgatagcg accgcgtttg gtttcatcga cgggaggaaa
    36181 gtcgttggct tcagccagac gctggacgtc gcgcagtccg gtatcaccgc tgatcgggcg
    36241 agccccttcg cgcaccagct tcatgccgtt ataatccatc ggattatggc tggcggtgac
    36301 ttcaatgccg ccatccacgc cgagatggaa cgtggcgaaa tagatctctt cggtgccgga
    36361 cataccgata tccagcacgt caacgcccgc atcctgtaaa ccttttgcca gcgccagttt
    36421 taaggtttcg ctggtgagac ggacatcacc gcctaacaca atggttttcg gtttgagaaa
    36481 ttcgccatag gcgcgaccaa tgcgccaggc gatatcttca ttcagttctt cgcctaattt
    36541 cccgcgaata tcataggctt taaagcaggt taattttttc atgtcgttac cctttttcag
    36601 gcaatagttg gccctgaccg aaacgaccaa ttttgttttt gtcgttattt acatctcggt
    36661 ttcgcattca ttgcctgatg cgacgctgcc gcgtcttatc aggactacaa acccgaaccg
    36721 taggtcggat aaggcgttca cgccgcatcc gacaaacagc gcctgatgcg acgtttacac
    36781 ccgtccgtag cgatccgcga agcgcaccac atcatcctct tcgagataag agccggagcg
    36841 cacttcaatt aaatcaagcg gaattttccc cggattttcc aggcagtgcg tcgcccccag
    36901 cggaatataa atggactcgt tttcaccaag cagtttgata tcaccatcaa tggtgacttt
    36961 tgccgttccc gcgaccacta cccagtgttc cgcgcggtga tggtgcatct gtaccgacaa
    37021 accctcgccc ggtttcacgg tgatgcgttt cacctggtag cggtcgcccg cgtcgataga
    37081 gtcatatttg ccccacggac gatacacttc gcgatgcacc cgatgctcat ggcgaccatc
    37141 ggctttgatc tgctcgacca cttttttcac atcctgtacc gcgttacggt cggcaatcag
    37201 caccgcatct ttggtctgca ccactaccag atctttcacc ccgacggtgg tgaccaggcc
    37261 agattcggca tacacatagc tgttttcagt tttgtgatta atcacatcgc cgtggcaaac
    37321 gttgccctcg gcggtgtggg cgctgatctc ccataatgaa gaccaggaac caacatcgct
    37381 ccagcccgca tccatcggca ccacaacagc atctgccgta cgttccatga ccgcgtaatc
    37441 caccgactct tccgggcagg cgagaaacgc ttcttcatcc acacgaataa aatcaagatc
    37501 cggatcgacg gagctcatcg ctttttcaca ggcatcgaga atatccgggc gatatttttt
    37561 cagttcttcg agatagcgtc cggcgcggaa caggaacatg ccgctgttcc agtaatattc
    37621 gccgcttgcc acataggcct gggcggtttc cagattcggt ttttcgacaa actgcgccac
    37681 ttcaaaggcc accgtatcct gctcacccgc agacacttca ccgcgacgaa tatagccata
    37741 accggtttct ggtagatccg gcacaatgcc aaaggtcacc agcttgcccg cttcggcata
    37801 tggcatggca ttacgcacgg cggcacggaa cgcgtcttca tcggcaatta catgatccgc
    37861 cgccagcacc agcattaacg ggtcgttctc cgggctatga cgttttgccg ccagcgccgc
    37921 cagcgcaatg gccggtgcag tgttacgccc tgcaggttcg agaataatgt tctcggtgag
    37981 tttgttcagt tgacgcagct gttccgcgac aataaagcgg tgctgctcat tgcaaatcac
    38041 caccgggctt tcgcactcca cgccgttcag gcggcagatg gtggtttgca gcatggtgag
    38101 atcgcctttc aggcataaaa actgcttggg gtaaagtacg cgggaaagcg gccataagcg
    38161 gctaccggag ccacctgcca tcacaactgg atagagtttc gactgcgcca taattatccc
    38221 cgaatatcat ttataaattg acgtaacacg ttctctttat cgagcgtgcg ttcggcatat
    38281 tcacgtgcca ccgtgttgtg tttgggcagc aggagcgcct gactaatccc cgccaccagc
    38341 gcctcgaccg attccggctc tacgcaaacc gcaatgcccg gaaaggtttc gcaaagctgc
    38401 cccagttctg tgtgggcttc tgcggtaatc accgcgttac cgcctaccgc cagaatattg
    38461 gtcagtttcg acggcaatac ggcatctgcc gcgccgcatt tttgcaccac cagatggcaa
    38521 tcgcccatct tcagcagtgc gggtaaagcg tcatacgatt gcagcgggaa aaattgcatg
    38581 ttgcgcagtc cacgctgctg cgccattttt tccagccgcg ctttgccgcc gccctgcccg
    38641 acaatggcaa aaatcagcgg ttcatcgcgc aggcgatcgg cagcttcaat aacgttttcc
    38701 agtccctgct tttcaccaat attgccggag taaagaatga tttttttgtt atccggtagg
    38761 ccaagctggt tacgaagggc atcaacatcg gcgtctgcaa catgctgaaa acgggcgatt
    38821 tccgaccagt tggggaagaa aatgatgtta tccgccgcca cgcctttttc gatggcttta
    38881 ttcatcatcg aacgcgaaat cgtggagacg ttatcgacgt tatgcagtcc gctacgttcg
    38941 aacgccgttg ccagctgtgc cactttgccg cctttgcctt ttccggcaag gcccagcccc
    39001 agcatggcgt ccacttcgta atcctgaata tgcagcacgg tacgcgcacc agagagtttc
    39061 gccagcaggc gcattcccgg cgtgcaaaac agcgttggca ccacgccgat aatgcgatcc
    39121 ggcttccagc gacgttgcgc catcaacggg aaaaaactgc tgacggcaaa accgccgaga
    39181 tgcaacaggc gtttcagggt gctcggctgt tttggcacgt acagcgggca gcgccacacc
    39241 gtggcggccc cctcttctcg tttgtagcgc taggcggaat agttctcgcc cacctgccac
    39301 tgcgggtagt aaggcggtgc ggtaataacc cgcacctcat gaccttgcgc cgccagccat
    39361 tccaccattt ctccggtgta tttgccgatg ccggttaact ccggcgagta gttaatgccg
    39421 tagaccagta ttttcataat ccgggtactc cggcacgctt ctcagcgaga aaataggcgc
    39481 ggctgttagc gtgaacattg tcactggcga gcagtgcgtc cggcgtcagc cagcggtaat
    39541 cgtcatgctg ctcatccggc agtaacagtt cttcttccgc tactctgaag cgaaaaccga
    39601 gcaccacata gtgagtggtg aaatccgtgc cagagaagtt atcgtcataa aagtgctgcc
    39661 agacaccgta aaactggcct gctgttatcg gcagacgcag ccccagttcc gccatcgtca
    39721 gccgctcaaa tgcggcttcc agcgtttcgt ctttctgcac gcgccctccc ggcacaaacc
    39781 agtaaccctg cgccgggcgg ttggttcttt tgccaggcag aaactcgccg cgactgttct
    39841 cgacaataaa gtcgagagag acaagcggtg tggagcgcac taccgtggca aagtcttcct
    39901 gacgtaaaaa catcattacc cccgaaagcg gtcttgattc tcaaggaacc actggtaagt
    39961 gctggcaagc cccgcttcca gtgagatttc gtgataccag ccaagctgat gcaggcgcgt
    40021 cacatcaagc agtttgcgcg gcgtaccatc cggtttactg gcatcaaaaa ccacccgacc
    40081 tttgtaaccc accactttgg cgatggtttg cgccagctcg cggatagtgc agtcaacgcc
    40141 cgtgccgacg ttaatgtgcg acagcatcgg ctgggtgttt tccagccaga cttcgtgcgc
    40201 cagctccatc acatgaatgc tcgccgccgc catatcatcg acgtgcagga attcacgcat
    40261 cggtgtaccg ctgccccaca ccaccacgtc cggcgcattc tgtgccgtcg cctcgtggaa
    40321 gcgacgcagc aatgctggga tcacatgcga attactcggg tggaagttgt catgcgggcc
    40381 atacaggttg gtcggcatga ccgagcggta atctcgtccg tactggcgat tgtaagattc
    40441 gcacagtttg atcccggcga ttttggcaat ggcgtaaggc tcgttggtcg gctccagcgt
    40501 gctctgcaat aactcgcttt ctgccatcgg ctgttttgcc agtttcgggt agatgcagga
    40561 cgatccgaga aacagcagtt tgttcacgtc gttctgatgc gcggcgtgaa tgatgttgct
    40621 ctcaatcatc atgttctggt agatgaaatc cgccggatag gtgttgttgg caacaatgcc
    40681 gcccactttc gccgccgcca gatagacctg gtcaatgcgt tcgctggcaa agaaatcatg
    40741 cacggcgcgg ctgtccagca ggttcagctc gtcgcgggta cgtaatacca gctccacatc
    40801 accgcgctgt tcgagctgcc gcttgatggc agaaccgacc atcccacgat ggccagcgat
    40861 aaaaatgcgt tgtttactca tgcttatgac tccagcgcga tcgccacgtc gtagccgtga
    40921 gatttcagca gagagtgttt tttcgccgct tcgaggtcat tagccaccat ttcagacacc
    40981 atctctctga gggtgatttc cggtttccag cccagttttt cgtgcgcttt ggtcgggtcg
    41041 tcgagcagcg tttcaacttc tgccggacgg aagtaacgcg gatcaacggc gataatcaca
    41101 tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg aaaccacaat gcccttctct
    41161 tcaacacccg tgccttcaaa gcgcagtttg atgcccagct gtgccgctgc catttccacg
    41221 aactgacgta cggagtactg aacgccggtc acgataacga aatcttccgg ctgttcctgt
    41281 tgcagcatca tccactgcat ttttacgtag tctttggcgt ggccccagtc acgcagggaa
    41341 tccatattgc cgaggtacag gcacgactcc agcccctggg cgatgttggc gattgcgcgg
    41401 gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg attcatggtt gaagagaata
    41461 ccgttacaag cgtacatgcc gtaggattcg cagtagttaa cggtgatcca gtaggcgtac
    41521 agtttggcga ccgcatacgg agatcgcggg tagaacggcg tggtttcttt ctgcggaatt
    41581 tcctgcacca gaccatacag ttcagaggtg gaagcctgat agaaacgagt tttcttttcc
    41641 agaccgagga agcggatcgc ctccagcagg cgcagcgtac ccatcgcgtc gacgtcagcg
    41701 gtatattctg gtgactcaaa agagaccgca acgtggctca ttgcgcccag gttgtacact
    41761 tcatccggct gtacttcacg caaaatgcgc gtcaggttag aggtatcact caggtcgcca
    41821 taatgcagat ggaatttcgg gttgcaggtg tgcggatcct gataaatgtg atccacgcgc
    41881 tcggtgttga atgacgatgc gcgacgctta ataccatgca cctcgtaacc tttttccagc
    41941 agaaactctg ccaggtaaga accgtcttgt ccggttacac cggtgatgag agcgactttt
    42001 gacatgtctt attcctctgt atttttgaat
//
LOCUS       DAJTNN010000010        41632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTNN010000010 DAJTNN010000000
VERSION     DAJTNN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.010"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..255
                     /locus_tag="OO150_000525"
     CDS             <1..255
                     /locus_tag="OO150_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5514777.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO150_000526"
     CDS             complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO150_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5514778.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO150_000527"
     CDS             complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO150_000527"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5514779.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2907..3182)
                     /locus_tag="OO150_000528"
     CDS             complement(2907..3182)
                     /locus_tag="OO150_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5514780.1"
                     /translation="MSRTIFCIFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO150_000529"
     CDS             complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO150_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5514781.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4423..5142
                     /gene="trmB"
                     /locus_tag="OO150_000530"
     CDS             4423..5142
                     /gene="trmB"
                     /locus_tag="OO150_000530"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5514782.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLNNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5142..5468
                     /locus_tag="OO150_000531"
     CDS             5142..5468
                     /locus_tag="OO150_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5514783.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5652..6371
                     /locus_tag="OO150_000532"
     CDS             5652..6371
                     /locus_tag="OO150_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5514784.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6547..7593
                     /gene="ansB"
                     /locus_tag="OO150_000533"
     CDS             6547..7593
                     /gene="ansB"
                     /locus_tag="OO150_000533"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5514785.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7710..8717
                     /locus_tag="OO150_000534"
     CDS             7710..8717
                     /locus_tag="OO150_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5514786.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQSAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OO150_000535"
     CDS             complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OO150_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5514787.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(10001..10594)
                     /locus_tag="OO150_000536"
     CDS             complement(10001..10594)
                     /locus_tag="OO150_000536"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5514788.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVTQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(10602..10892)
                     /locus_tag="OO150_000537"
     CDS             complement(10602..10892)
                     /locus_tag="OO150_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5514789.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OO150_000538"
     CDS             complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OO150_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5514790.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGMWMAL"
     gene            complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OO150_000539"
     CDS             complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OO150_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5514791.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            12195..13175
                     /locus_tag="OO150_000540"
     CDS             12195..13175
                     /locus_tag="OO150_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5514792.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OO150_000541"
     CDS             complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OO150_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5514793.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(13767..14330)
                     /locus_tag="OO150_000542"
     CDS             complement(13767..14330)
                     /locus_tag="OO150_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5514794.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTASADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OO150_000543"
     CDS             complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OO150_000543"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5514795.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OO150_000544"
     CDS             complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OO150_000544"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5514796.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OO150_000545"
     CDS             complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OO150_000545"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5514797.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(17015..17512)
                     /locus_tag="OO150_000546"
     CDS             complement(17015..17512)
                     /locus_tag="OO150_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5514798.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OO150_000547"
     CDS             complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OO150_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5514799.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OO150_000548"
     CDS             complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OO150_000548"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5514800.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(20877..21092)
                     /locus_tag="OO150_000549"
     CDS             complement(20877..21092)
                     /locus_tag="OO150_000549"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514801.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            21228..21359
                     /gene="yqgB"
                     /locus_tag="OO150_000550"
     CDS             21228..21359
                     /gene="yqgB"
                     /locus_tag="OO150_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5514802.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            21368..23344
                     /gene="speA"
                     /locus_tag="OO150_000551"
     CDS             21368..23344
                     /gene="speA"
                     /locus_tag="OO150_000551"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5514803.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            23481..24401
                     /gene="speB"
                     /locus_tag="OO150_000552"
     CDS             23481..24401
                     /gene="speB"
                     /locus_tag="OO150_000552"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5514804.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OO150_000553"
                     /gene_synonym="yggG"
     CDS             complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OO150_000553"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5514805.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            25643..27634
                     /gene="tkt"
                     /locus_tag="OO150_000554"
     CDS             25643..27634
                     /gene="tkt"
                     /locus_tag="OO150_000554"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5514806.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            27948..28391
                     /gene="cmtB"
                     /locus_tag="OO150_000555"
     CDS             27948..28391
                     /gene="cmtB"
                     /locus_tag="OO150_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR5514807.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISHG"
     gene            28419..29807
                     /gene="cmtA"
                     /locus_tag="OO150_000556"
     CDS             28419..29807
                     /gene="cmtA"
                     /locus_tag="OO150_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR5514808.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSLGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDTGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            29822..31099
                     /locus_tag="OO150_000557"
     CDS             29822..31099
                     /locus_tag="OO150_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5514809.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            31096..32061
                     /gene="yggF"
                     /locus_tag="OO150_000558"
     CDS             31096..32061
                     /gene="yggF"
                     /locus_tag="OO150_000558"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5514810.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            32083..32592
                     /gene="fumE"
                     /locus_tag="OO150_000559"
     CDS             32083..32592
                     /gene="fumE"
                     /locus_tag="OO150_000559"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5514811.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            32589..33302
                     /locus_tag="OO150_000560"
     CDS             32589..33302
                     /locus_tag="OO150_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5514812.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(33274..33951)
                     /locus_tag="OO150_000561"
     CDS             complement(33274..33951)
                     /locus_tag="OO150_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5514813.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(33945..34622)
                     /locus_tag="OO150_000562"
     CDS             complement(33945..34622)
                     /locus_tag="OO150_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5514814.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(34610..35317)
                     /locus_tag="OO150_000563"
     CDS             complement(34610..35317)
                     /locus_tag="OO150_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5514815.1"
                     /translation="MHPFTSLILWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(35318..35896)
                     /locus_tag="OO150_000564"
     CDS             complement(35318..35896)
                     /locus_tag="OO150_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5514816.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(35919..36350)
                     /locus_tag="OO150_000565"
     CDS             complement(35919..36350)
                     /locus_tag="OO150_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5514817.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            36722..37741
                     /gene="epd"
                     /locus_tag="OO150_000566"
     CDS             36722..37741
                     /gene="epd"
                     /locus_tag="OO150_000566"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5514818.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            37791..38954
                     /gene="pgk"
                     /locus_tag="OO150_000567"
     CDS             37791..38954
                     /gene="pgk"
                     /locus_tag="OO150_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5514819.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            39169..40248
                     /gene="fbaA"
                     /locus_tag="OO150_000568"
     CDS             39169..40248
                     /gene="fbaA"
                     /locus_tag="OO150_000568"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5514820.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            40606..41466
                     /gene="mscS"
                     /locus_tag="OO150_000569"
     CDS             40606..41466
                     /gene="mscS"
                     /locus_tag="OO150_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5514821.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
BASE COUNT        10191 a        10409 c        11087 g         9945 t
ORIGIN      
        1 attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa
       61 ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg
      121 gcggttcaac gttatttcct tgcactggaa gaaggggtaa atttgttgcc gggattttcg
      181 ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt
      241 tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc
      301 gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca
      361 tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac catacggtct
      421 gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc
      481 cgaggataca gccgaagccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa
      541 ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg
      601 cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc
      661 cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct
      721 taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct
      781 gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca aacatcggat
      841 ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc
      901 ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac agcgcgaatg
      961 catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat
     1021 gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct
     1081 gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga
     1141 tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca
     1201 ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa
     1261 tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg aacagcgtga
     1321 tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca
     1381 caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta taaactgcgc
     1441 caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc
     1501 aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca
     1561 tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg
     1621 agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg
     1681 caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca
     1741 tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt
     1801 tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat
     1861 cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc
     1921 gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc cgcgatgttg
     1981 ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg gtgccggtat
     2041 caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg
     2101 agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc agcgcatcgg
     2161 aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc agaatcagcg
     2221 actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac
     2281 gtttatcaag gtggttcggt accatgttaa tggtgacgct gtagatgata cgcagcccgt
     2341 tactgcggct ttgcagacgg tttttcagca gataatccgc gaagttgctt gcgcgacctt
     2401 cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg
     2461 aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg tcacccatca
     2521 gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg gcgatagttt
     2581 cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt
     2641 atttcacgta gtccttagga ccggcgatca ccacctcttt gaatccccag atgttctcaa
     2701 tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg acccaggctt
     2761 cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag
     2821 ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt
     2881 gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata gccctcgata
     2941 tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag cttgcggtgc
     3001 tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt gtgctgccac
     3061 tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc gccggggtac
     3121 agctgaaaat cctgaccttc tgcttcacgt tgcaggaaaa tacaaaaaat cgttctgctc
     3181 ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg taactgctgt
     3241 aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa gttataccag
     3301 agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca cataggcaca
     3361 atatctaagt ggaaatggct gaaggtatgc cgaaacgcgg tcagttgcgt caggttatcg
     3421 gcagcaatct gccgttgcgc cagccactgc cgcaaacttt cttcgtcggc aaactgcggg
     3481 aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa tacttcatct
     3541 tcgtgctgta acagcaaaaa gtagccggtg cgctccggca gcgtctgttt cggttttttg
     3601 cccggataaa gcgaccagct attattggcg gcggcaatac atccgttttg tagcggacag
     3661 agcgaacatt tcggcttcga gcgcgtacaa atcatcgcgc ccaaatccat catcgcctga
     3721 ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca taatttattc
     3781 tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag cacgcgtttg
     3841 acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg
     3901 gtggaacgcc cgacgcccgg taacgccgcg acttcttcaa aggtttccgg gaatttaccg
     3961 ccgtgtaagg tcgccacttg ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag
     4021 ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag atcggtcacc
     4081 gtcgggaagc gcgccataaa gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt
     4141 tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg ccagggcagc
     4201 gtttttcgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc
     4261 atggtcaccg aattcactgt tgttgggggc aagattgcag cacagcgacg gcagggtgta
     4321 aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact aactttggat
     4381 aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt
     4441 tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc
     4501 caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc ggtgatgggc
     4561 gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga agcgccggtg
     4621 acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa agatcgccct
     4681 gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct
     4741 gcgcatgaag aaggtttaaa caacctgcga gtgatgtgtc acgatgcggt tgaagtgctg
     4801 cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc tgacccgtgg
     4861 cacaaagcgc gccataataa acgccgtatc gtacaggtgc cgtttgccga actggtaaaa
     4921 agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc ttatgcggaa
     4981 catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga gagcaatgat
     5041 tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt
     5101 ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag aaccgtagcc
     5161 gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt tcggtggcat
     5221 ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat gattttatta
     5281 acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg gcctgggaag
     5341 gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg attgtgcgta
     5401 agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc gacgtttggt
     5461 gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat
     5521 caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc gcatccggca
     5581 tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg attttttctt
     5641 gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg acggcaatga
     5701 ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc
     5761 aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac ggcaacgtga
     5821 tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg aaggattatc
     5881 aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaaa
     5941 aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgaaagc agcaaaatgc
     6001 gcagccgtct gaccaaactt gatgcgcaac tgaaagagca gatgaaccgc attatcgaaa
     6061 cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc gccgaaggcc
     6121 agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat gaaatgggcg
     6181 cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga agcctggggg
     6241 ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc cagcagtttg
     6301 gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc ctggtcggga
     6361 atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt ttaattcttc
     6421 gttttgttac ctgcctctaa ctttgtagag ctccaaaata tattcacgtt gtaaattgtt
     6481 taacgtcaaa tttcccatac agagctaagg gataatgcgt agcgttcacg taactggagg
     6541 aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg ttttagtggt
     6601 gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat tgccggtggt
     6661 ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga aaatctggtt
     6721 aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt agtgaatatc
     6781 ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat taacaccgac
     6841 tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga agaaaccgct
     6901 tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgc
     6961 ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt agtgaccgca
     7021 gctgataaag catccgctaa tcgtggcgtg ctggtggtga tgaacgacac cgtactggac
     7081 ggtcgcgatg taaccaaaac caacaccacc gacgtagcga ccttcaagtc tgttaactac
     7141 ggtcctctgg gatacattca caacggtaag attgactacc aacgtacccc ggcacgtaag
     7201 cacaccagcg atacgccatt cgatgtctct aagctgaatg agctgccgaa agtcggcatc
     7261 gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga tgcgggctat
     7321 gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt ataaatccgt gttcgacacc
     7381 ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt cttcccgcgt accgacgggt
     7441 gctaccactc aggatgctga agtggatgat gcgaaatacg gcttcgtcgc ctctggcacg
     7501 ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc tgacgcaaac caaagatccg
     7561 cagcagatcc agcagatctt caatcagtac taatcacctc gctccggtat cgtgccgggg
     7621 ctttttcact tcagacttac gtccattgcc aattttcatt accctaatga taatcaccgg
     7681 aataaattat ttcgcgtgag gtttttcggg tgaaaaagca atggattgtt ggcacggcac
     7741 tgcttatgtt gatgactggt aatgcctggg cagatggcga accgccaact gaaaatatct
     7801 taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgct atcaccttaa
     7861 aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat gtctcttcca
     7921 gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc gaacagacct
     7981 ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga acgcccgcgt
     8041 ctggctggtc ggtgaacttt tactcttttc agtcggcagc cagcgatcgt gggcgggtgg
     8101 tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat ttcaattatc
     8161 gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct gcgctggaaa
     8221 aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg gatgcatatt
     8281 ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa aaaattcacc
     8341 aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt aaatatgaca
     8401 aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag tgttatgaag
     8461 tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag acttttctgc
     8521 aatcacaaaa actcagccgt aaattgcagg atgactgggt aacgctcgaa aaagggcaat
     8581 atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt ttgatgaaaa
     8641 ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa ctgcggcgca
     8701 atggggtgat taagtaagtt tgatatagtg cacgatttgt agcaggatgc ggcgttgacc
     8761 ccgcatccgg caatcaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgt
     8821 tgccacatgt aggctggata aggcgttcac gccgcatccg gcaatacaag tttactcagc
     8881 cagaaaaagc tccagcagcg aatttaaaaa cagcttccca tgttccgtta tctgccagta
     8941 atccgcacat tctgtgagat aaccctgggc aatcgcctcg tctaactgtg ggcgaatcac
     9001 atcttcgcaa agcccggtat acgcactaaa ctctgcgcgt ggcgcggcct ccagcaagcg
     9061 aaagcggttc ataaagaact caaacggctt atctgcggct tcgacatcac gctggctttc
     9121 cagatacctt ccttgcataa aaccacgagg atgacgcgtt ttagtggtac gcagaatgcg
     9181 cccatccggg aaggtgactt tgccgtgcgc gccgcagcca ataccgatgt agtcaccaaa
     9241 tcgccagtag ttgagattgt gctggcactg ataaccgggt ttggcgtaag cggaagtttc
     9301 atattgctga taacccgctg cggttaataa ctgatgcccc tgttcgaaaa tatcccacag
     9361 cgcgtcatcg tccggcagca ccggtggtcg cgaaccaaac agcgtattgg gttcgatggt
     9421 cagttgatac caggaaagat gcggcggatt cagttcaatg gcctggcgta gatcgccaag
     9481 cgcctcttcc agtgattgat ccggtagtcc atgcatcaaa tcaaggttaa agctacgtaa
     9541 ccctaaaccg ctcgccagct ttgccgctcg tttcgcttct tgcgggccat gaatgcgccc
     9601 aagtcgtttc agcttttctt cgctaaaact ctgcacacca atagagatgc ggttcacacc
     9661 agcacgctga taatcgacaa agcgatcggc ttctaccgta ccagggttcg cttccatagt
     9721 aatttctgca tccgctgcca gcggcaaacg cgcacgcacg ccgtccagca gcgtttgcat
     9781 cgccgggccg gaaagcaggc tcggcgtacc accgccaata aagattgtct ttacttcacg
     9841 gccctgagcg taagccacat cattgtccag atcgcaaagc agatgctgaa cataatcgtc
     9901 gtgcggcact tctcctttca acgcgtgaga gttgaaatcg cagtacgggc atttctgcac
     9961 gcaccacggg atgtgaatgt agagactcag cggcggtaat ttaaccatta cgtaaagcgt
    10021 ccagcagcag tttcaacgcc tgaccacggt gggaaatggc gctcttttct tcgcgggtca
    10081 gttcggcggc ggttttccct tcggaaggta cgaagaagat tggatcataa ccaaagccac
    10141 cagtgcccgc tggttcgcga gtaatcacgc ccggccagct gccgtggcat accagcggag
    10201 tcggatcttc cgcgtgacgc agatatacca gcacgcagtg gaaacgtgcc tgacgttggt
    10261 cgtccggtac gtctttcagt gtttccaaca gtttttgcag attcttttga tcggtcgcgt
    10321 cttcaccgga ataacgcgcg gagtaaatac ccggcgcgcc acccagcgca tctaccgcca
    10381 gaccagagtc gtcggcaatt gccggtaaac cggtcacttt tgccgcatgg cgcgctttca
    10441 gaatcgcgtt ttcgataaag gttaggccgg tttcttcagc ggaatcaacg ccgaggtctg
    10501 tttgtgtcac gatatcaaga ccaaagtcgc taagcagcga cgccagctca cgcactttac
    10561 cggcattgcc ggttgcgagg acaacttttt gcataggata cctaattaat taacgccgcg
    10621 atttctggcg ggatttgttg cggattaatg attttaattt gtttgtggcg gccaagttcg
    10681 cctttttcaa tcaccacctg gcttttggca acccggaatt gcttaccgag aaacttcacc
    10741 agatgactgt tggcctggcc gtcaactggc ggcgcggtaa tggcgacttt aacttcgtcg
    10801 ccatgtaaac cgacaataga atcacggctg gctttcggct gaatatagag ccgtaaaacc
    10861 agaccgtcat cattaactgt tacggcattc ataacgccat ccacatcccc ggcagcagga
    10921 tgtttccggt tgcctgtaat acttctgcga cacccatatt gatgacatac agcagcagaa
    10981 cgaggatcat cggcgagaaa tcaatcccac ccattgccgg tagcaagcgg cgaatcgggc
    11041 gcagcagcgg atcggccagc tgaatcagca cgtattcaat cgggctacga ccctggctta
    11101 cccagctcat aatcgccatc accagcagga cccagaaaat cagcaagccg atggttttca
    11161 gcagaatcag taaaccggca atccagatga ttggcaggaa ggtcaccact ttaaacagca
    11221 caatggcttt gataaaactg agaatatagg caaccagcag cgaggcgctg tcaatgggac
    11281 ccattgccgg aataacgcgg cgcagtggcc cgataattgg ctgcgttacc ttcactacaa
    11341 actgtgagaa ggggttgtaa aaatcacaat gagcccactg catccagatg cgtaataaca
    11401 gcaccatggt atacagctca atgaccgttg aaagcaggaa agtcaacgta ttcatggcgt
    11461 tcctttaatt ccttattttt tagagtaatc acgcgcacca aaaattgcag tgccgatacg
    11521 aaccatcgtg ctacctgccg caatggcggc ttccatatcg tccgacattc ccagagagag
    11581 cgtgtcgata tgcgggtagc gcgttttcag tccggcaaat gctacagcca tttggcgtgc
    11641 aacttcaaac tgccttacat attctgactc aggcgcaggg attgccatca gcccgcgcag
    11701 acgtaaacgc ggtagttcag cgaccgccgc tgccagctcg tccagttctg ccagttgaat
    11761 cccggactta ctgttttcat cactaatatt aatttgaatc agtacgttaa gagggggaag
    11821 ttctgccggg cgctgatcgt tgaggcgggt agcaatgcgc aaacggtcga tggtatgaca
    11881 ccagtcgaaa tgctctgcca ccaggcggct tttattagac tgcaacgggc caataaaatg
    11941 ccattctaat cctgttacgc ccagttcctg aaagtggcga attttatcta ccccttcctg
    12001 aacgtagttt tcaccaaatt gacgctgccc ggcatcaatg gcttctgcga tggcgctcgc
    12061 aggttttgtt ttactgactg caagcagcgt aatttcttct ggagaacggc cgcaacgcgt
    12121 tgcagccgct gagattttgt cccggacctg tgccaggtta tgcgcaatat cgttcatttt
    12181 ccgaggatct tagtatgaat atggaagaaa ttgtggccct tagtgtaaag cataacgtct
    12241 cggatctaca cctgtgcagc gcctggcccg cacgatggcg cattcgcggg ctaatggaag
    12301 ctgcgccgtt tgatgcgctg gacgtcgaag agctactgcg ggagtggctg gatgacgatc
    12361 agcgggcaat attgctggaa aatggccagc tggattttgc cgtgtcgctg gcggaaaacc
    12421 agcgattgcg tggcagcgca ttcgcgcaac ggcatggcat ttcgctggcg ttacggctgt
    12481 taccttcgca ctgcccgcag ctcgaacagc ttggcgcacc aacggtattg ccggaattac
    12541 tcaagagcga gaatggcctg attctggtga cgggggcgac ggggagcggc aaatctacca
    12601 cgctggcggc gatggttggc tatctcaatc aacatgccga tgcgcatatt ctgacgctgg
    12661 aagatcctgt tgaatatatc tatgccagcc agcgatgttt gatccagcag cgggaaattg
    12721 gtttgcactg tatgacgttc gcatcgggat tgcgggccgc attgcgggaa gatcctgatg
    12781 tgattttgct cggagagctg cgtgacagcg agacaatccg tctggcactg acggcggcag
    12841 aaaccgggca tttggtgcta gcaacattac atacgcgtgg tgccgcgcag gcagttgagc
    12901 gactggtgga ttcatttccg gcgcaggaaa aagatcccgt acgtaatcaa ctggcaggta
    12961 gtttacgggc cgtgttgtca caaaagctgg aagtggataa acaggaagga cgcgtggcgc
    13021 tgtttgaatt attgattaac actcccgcgg tggggaattt gattcgcgaa gggaaaaccc
    13081 accagttacc gcatgttatt caaaccgggc agcaggtggg gatgataacg tttcagcaga
    13141 gttatcagca gcgggtgggg gaagggcgtt tgtgacgttg ccatgcccga tgcgcaagtt
    13201 tatagggcct ataaaggcaa taaatgtctg atgcgacgct aacgcgtctt atcagaccta
    13261 cagccgctgc cagatgtgtg ctggataagg cgttcacgcc gcatccggca agatggcggt
    13321 aacgactatc cgccaggctt ttagatcgcc ttaatatccc tgctcgaaat agctttcgag
    13381 aataataacc gcagaggcag agtcgacttt gcctttgttg agcgcccgat agccgccctg
    13441 ttcaaacaga ccggaacggg cttccacggt gctaagacgt tcgtcatgga gctttacttc
    13501 aacaccgaaa cgaccatgaa tacggttggc aaatttacgc gctctggcag tcaatggttg
    13561 ctcggtgccg tccatattca gcggcaaacc gacgatgatt tcgtccggct gccactcttt
    13621 cagtaaacgt tcgatgaggt tccagtccgg cgtaccgtcc tgtgctttaa ttgcaggcaa
    13681 agggcgagca gtgccggtaa tgcgttggcc gaccgctacg ccaatgcttt tggtgccgaa
    13741 gtcgaaggcg agtaaggttc cactcatcag gcgtgccctg ccacaccagg catggtgaga
    13801 atatccacac caatcagttt tgccgcctcg cgccagcggt cggcaatcgg cgttttgaac
    13861 agaatattca gatccgccga ggccgttagc cacgcgttat cgagaatttc ttgttccagt
    13921 tgaccttttt cccaggaggc atagcccaga gccaccaata cgtcagacgg ttgtttatcg
    13981 gtgccgagcg tttccagcac atcgcgggaa gtggtcatca ccgtgttgtc tgaaatgcga
    14041 atgctggaag caaaattgga gggcggagta tgcaaaataa acccgcgatc ttcagccagt
    14101 gggccgccga gcataacagg tttatccaga cggattgatt catcacgcgg ctccggcgtg
    14161 atcttcagct tttccagaat cccttcaatt ttgagatttt ccagcggctt gttgacgatg
    14221 atccccattg caccattggt attatgttcg caaatgtaga ccacggaacg acggaaaatc
    14281 ggatcctgga gagcaggcat ggcaataaga aagtgatgct gtaaattcat tgtcagaggt
    14341 tctgtttcct ggttcaaaaa agcaacagcg cccagtatgc gtggaaagaa gggcgctgtc
    14401 actcagagtc tcaacgagat ccttctcgct aaggtgggtt actgctgctg taaacgtgct
    14461 tcgatggcat ccattaacat tccggtgatc gacaccggaa actctgcttc aatctcacga
    14521 atacaggttg ggctggtgac gttaatttca gtcagacggt cgccgatgat atccagacca
    14581 acaaaaatca gccctttttc tttcagcgtc ggcccgatct gacgggcgat tttccagtca
    14641 ctttccgtca gcggccgagg ttcaccgcga ccaccggcag ccagattgcc acgggtttcg
    14701 cccccctgcg gaatacgcgc caggcagtac ggtaccggct cgccatccac caccagcacg
    14761 cgtttgtcgc catctttaat ggctggcagg taattttgcg ccatgcagta gcgagtgcca
    14821 tgctcagtca gggtttcggc aatcacgccg aggtttggat cgccttcttt cacgcggaaa
    14881 atcgacgcgc cgcccatacc gtccagcggc ttaagaatga tgtcgctgtg tttctcccag
    14941 aacgctttta gctgcgcttt attgcgcgta accagcgttt ctggcgttaa gtcagagaac
    15001 caggcggtaa acagtttctc gttacagtcg cgcaggctct gcggcttgtt aacgatcagc
    15061 gtccctttct cttcggcacg ttccagaata taggtcgcgt agataaactc ggtatcaaac
    15121 ggcgggtctt tacgcatcag gagcacatcg agatcggcca gcggcagatc ctgttcaccg
    15181 acgaacgaaa accactcttc gtagttctgc ttcacgttca gcgtgcgggt atgggcgcga
    15241 gcttcgccat tgatcagata cagatcgccc atctccatat agtgaagttc gtaaccacga
    15301 cgctgtgctt ccagcaacat agcgaaactg gaatctttct tgatgttgat gtttgcgatg
    15361 gggtccatca cgatgccgag cttgatcatt attcttctcc gttagcccaa atcgccaaat
    15421 cgtacttgta gcgcggtaat ggcggtgagc gcagttgtct ctgtacgcaa aacgcgaggt
    15481 cccaacagga tatcagtaaa ttgatagcgg gcagtcatgg caatttcatc tgccgataaa
    15541 ccgccttccg ggccaatcag caggcggacg cgttcaaccg gtaacggcaa cgtattgatg
    15601 ctgttactgg cgcgcgggtg aagattcagt ttcagtcctt catcctgctc tgcacaccag
    15661 gcttccagat ccatcgctgg acggatttcc ggcacccggt tacgaccaca ctgctcacag
    15721 gcagcaattg caatcttctg ccactgctgg agcttcttgt tcagacgttc actatccagt
    15781 ttaacgccgc agcgctcaga aaaaagtggc gtaatgaggc ttacaccgag ttcgatcgat
    15841 ttctggatcg taaattccat tttttcacca cgcgacatca cctgaccgag gtgaatatgc
    15901 agcggagatt cgcgatcgtc gatcttgcct tccagcacct tcacttccac gctttttttg
    15961 ctggcgctgg taatttcggc gtcaaagacc tggttgctac cgtcaaacaa ttgcaacgcc
    16021 tgccccggcc ccatgcgcag tacgcgcccg atatggttgg cggcatcttc gcaaagcgcg
    16081 atgtgagaat ggctggtcag tggttcagga tgataaatgc gggggatacg catagttaaa
    16141 aatccgcgtc gtctccccac gcggttgtac gcgtggggta ggggttaaca aaaagagtcc
    16201 cgctagtgta ggttagctct ttcgcgcctg gcaagcgcgt tgcacatacg ggttatgatt
    16261 gccttgcacc ttcgcgatgc gttcatcgcg ctcgcactcc cagtcggtaa ccggatacat
    16321 cttgttccat gcgttgaaca gctgcgtttg ctggcgagag agtgtcaggt tgtattggtc
    16381 gcgcatatag aagtaggtgc gcgcaatggc accgcgtgca cgcgctggtg gttcggcagc
    16441 tttttctttg aaatcgacct tcatggcgca ttgaccgtac tggccttcgc cgccattcca
    16501 ctggctgtac ataaagttgc cgcgatcgcc attcacctca ccgactgacg gctgcaggtt
    16561 atgcatatcg ctttccatct tgcgatagac cggatcttta gcgcagtttt tacgtccacc
    16621 gtcctgccag cactggcgct ggtgaccgaa ctgccaggcg ggaacgacgt gttcccactc
    16681 tacgcggctg gctcggtttt catttttgcg cacctgatag ccgcacgatt gcagatcaac
    16741 aacgcctttt ttgccctgcc agttaatttt acatccgcaa taaaacgtac cgggcgcgtc
    16801 agcgtggact tttaccgccg cggctttcgc ctgagaaaaa ctattgatac cttcggccaa
    16861 cgccgggccg gaaaatgctg cgctcagtac caccgcagca atagacaaat aacggtacat
    16921 cacttactcc gtgttaaaac gagccagcaa cgtagcgaaa gttgttttgg tatgcaatca
    16981 gccagatcag gaaagttcct gatagttcag atggttattt tgcgaccagt tgttcgccgc
    17041 agtgaacaca gcgatagacg gcttcgccac gaactacgcg attatggcgg cgtacggtaa
    17101 gctgatgctc ctggcacttg cagcggtagg ggaaggtgtt gcgacgcacg gattgcagtt
    17161 cgaactgatg cgtacgacgg gcgggaacac ccagcacgtt ttccatcatc cacttccact
    17221 ctttgccatg tggtgctacg cgcccgaaat gtttccatac cagcaaatgt gccagttcgt
    17281 gcggcaccac ttcttcaata aaggcttcac tgttttccaa cagcaaaacg ggattgaggc
    17341 ggatttcata gctttccagc caggctgttc cggcggaggt gccgcgctgg gtataagaga
    17401 gttttggctc cgggtagtta cgccccaatt tcaggttagc ctgggcgagt ttttcccgca
    17461 ggcgacgcat aacggcctgt tggatggcga tagggagacg ggatgttttc atagcggcag
    17521 aggatagagc gaagaaaaga tgactgcaag aggtggcttc ctccgcgatg ggaggaagct
    17581 tggggagatt aatcgtgagc gcctatttcg cgcagtttac gacctttcat cagattacgt
    17641 tcaatatgtt ccagcgaaac gtgtttggtt tccggtacca gccacaatgt cagcaggata
    17701 aacagtacgt tcagagccgc atacacccag aaggtgttgg cgttacccag tgtgttgagc
    17761 atggtcagga acgttgcgcc aacgatcatg ttggcaatcc agttggtggc ggtagagcag
    17821 gtgatgccaa aatcgcggcc tttcagcggc tgaatttcgg agcacagtac ccaaatcagc
    17881 ggaccggcac tcatggcaaa accgacaata aacatcagca gcatggcgat ggcgaaatac
    17941 tgcgctgacg gagagtgaat accgatatgc atcattgtac cgagtacgcc catgccagca
    18001 gccatcacca ggaagcccag cgttagcgtt ggtttacgtc cccagcggtc aacaaggccg
    18061 attgcgataa aggtggcaag tacgttggtc aggccgacaa tcactgtccc ccacatttgc
    18121 tcggtagtgt tggtataacc cgccagttcg aagattttcg gcgcgtaata catgatgacg
    18181 ttcatcccgg tgaattgctg cattacctgt aacagtacgc caaggaacac cgcgcggcgg
    18241 aagttgctat tctctttaaa cagcgcccag ccactctgtt taacctgcaa actttcacgg
    18301 atttcatcca gttcgcgttt cgcttccgcg ctggtgtcac gcaggcgtag cagcacgcgt
    18361 tcggcatcaa caaaacggcg tttggcggca aaccaacgtg ggctgtctgg caggaagaag
    18421 acaccaatca gcagcaaaat tgccgggatg ataatcacac ccagcatcca gcgccatgca
    18481 ccggtgtagc tgaaggcggt atcagaaaga taagcaccga ggatcccgat agtgatcatc
    18541 aactgataca tcgagatcat actgccacga attttttccg gcgcaatttc agagaggtac
    18601 agcggtgcgg tataagaggc cacacccacc gccagcccca gtagaacgcg ggaaagaatc
    18661 agtacttcaa cgtttggcgc agccgcagag aacagcgaac cggcaacaaa caaaattgcg
    18721 ccgatcatca ggctcttttt gcgcccgagt ttaaaggaga gccagccgct gcccaccgca
    18781 ccgactgccg caccgaacat catggagctt acgacccatt cttgcgtgtg cgaagtaatc
    18841 tggaattcat ctgcaataaa cggcagtgcg ccagcaatta caccgatatc caggccaaag
    18901 agtaatcccg ccagagcggc aaggaagcag acgaaaaacg tcattgcctt gtttgaccgc
    18961 ccctgttttt tagcgtcagg catgatgccc tccaatatgg ttatttttta ttgtgaatta
    19021 agataggtga gtacgacgta aaaagatgtg aagcaaatca catcagtgta atcgcttaca
    19081 ctaacattta tcttagttat ggtttaagtg catgaaaata attgagttat atcatcatag
    19141 attaaacgct gttatctgca attaagactt tactgaaaag aaatgtaaca actgtgaaaa
    19201 ctgaataata atatgaaaat attacaaata tttggctggt gttattggtg taatcggttt
    19261 cagtagtgat gataatttgc gataaaacgt gttgaggaag aatgatgtta ttgtgcgggc
    19321 agctttttgc cggatgcggc gtgaatgcct atccaccgcg gtgaggtaaa agaaaaaggc
    19381 cagcgcgagg ctggcctttg aaagcaggtg aagaaagatt acttcagacc ggcagcatcg
    19441 cgcagcagct gcgctttgtc ggttttttcc cacgggaaat gttcacgacc aaagtgaccg
    19501 tatgctgcgg tttctttgta gatcgggtgc agcagatcca gcatctgaat cagaccgtat
    19561 gggcgcaggt cgaagaactc acgtaccagc agagtcagtt gttcagaagg cactttctca
    19621 gtaccgaaag tttctaccat gatggaggtc ggttcagcca cgccgattgc gtaggaaacc
    19681 tgaatttcac aacgatcggc caggccagca gcaacgatgt ttttcgcgac ataacgtgct
    19741 gcgtaggctg cggaacggtc cacttttgat ggatctttac cagagaatgc tccgccaccg
    19801 tgacgtgcca tgccgccgta ggtatcaacg ataattttac gaccagtcag accgcagtca
    19861 cccatcgggc caccgataac aaaacgaccg gtcgggttga tgaagaatgt ggtggcagaa
    19921 gtcagccatt cagcgggcag aattggcttg atgatctctt ccattaccgc ttcttgcagc
    19981 gatttctggt cgatctcttc agagtgctga gtggaaagca cgacagcatc aataccaacg
    20041 attttgccgt cgtcatactg gaaagtcacc tggcttttcg cgtccgggcg cagccacggc
    20101 agagtgccgt ttttacgcac ttcagcctga cgctgtacca gacggtgtgc ataggtgata
    20161 ggtgctggca tcagcacgtc ggtttcatta gttgcgtagc caaacatcag accctggtca
    20221 cccgcgccct gttccagcgg atcggcacgg tcaacgccct ggttgatgtc aggagactgt
    20281 ttgccgatag cgctcagaac cgcacaggag ttagcgtcaa agcccatgtc ggaatgcaca
    20341 tagccaattt cgcgaacggt gttacgggtg atctcttcga tgtctaccca ggcgctggtg
    20401 gtgatttcgc cgccaactaa aaccatgccg gtttttacgt aggtttcgca agcaacgcgt
    20461 gctttcggat cctgttcgag gatcgcgtct aaaacggcat cagagatttg gtcagcaatt
    20521 ttgtcaggat gcccttcaga gacggactcg gacgtaaaaa ggtgttttgc catatttaat
    20581 atcacctaaa gagaatttgg ttagctcaaa ctgttgtgtg gattttctgt ggtagcggat
    20641 cctaccacga ctctgcaggt taaaaacact ggcagtctga gtgttaatcg gtatggatgg
    20701 attaacatct ggatggctat tttaggtcaa ttcttcaccc gatttccact tttttttgaa
    20761 tcgtgtctca ttctgttaaa aacgtggctg gaaatttttt ctgacaatgc cggcattctg
    20821 cgtatttata ttttgcaatt ttctgccatt gtggggtata aaacgcggcg cgcggcttaa
    20881 ataaaaagca cacgacgttt ctttcgtgtt gccacttcca gccgggttga aatcagagtt
    20941 ttggcttgtg ggttcgtctt aacaggcggc cgtggaggtg atacgaaata atgaaccgta
    21001 gtctgctgct taacctgtct caccgttctg gtgaagattc gttccccgca ctctgcatct
    21061 ctgctttgca tacctgccga tgttataccc atctcggcgc ttctcaggat tcaagagctg
    21121 gttacagtta ctgaggactg aacaagggcg ctcttgtaaa aacaagagtt ttctcgtggt
    21181 ttcgccgaac tgtcacactt acgttcggtt atgtgcttaa taatgttatg aaaaagaaac
    21241 cggttgcgca gttggagcgt cagcattcac tgctggaaaa tccatgtgct tatgggttgt
    21301 tatcgcagtt ccaggctgcg atagtcgtta actgttttac acttaataaa ataatttgag
    21361 gttcgctatg tctgacgaca tgtctatggg tttgccttcg tcagcgggcg aacacggtgt
    21421 actacgctcc atgcaggagg ttgcaatgag ctcccaggaa gccagcaaga tgctgcgtac
    21481 ttacaatatt gcctggtggg gcaataacta ctatgacgtt aacgagctgg gccacatcag
    21541 cgtgtgcccg gacccggacg tcccggaagc tcgcgtcgat ctcgcgcagt tagtgaaaac
    21601 tcgtgaagca cagggtcagc gtctgcctgc actgttctgt ttcccacaga tcctgcagca
    21661 ccgtttgcgt tccattaacg ccgcgttcaa acgtgcgagg gaatcctacg gctataacgg
    21721 cgattacttc cttgtttatc cgatcaaagt taaccagcac cgccgcgtga ttgagtccct
    21781 gattcattcg ggcgaaccgc tgggtctgga agccggttcc aaagccgagt tgatggcagt
    21841 gctggcacat gctggcatga cccgtagcgt catcgtctgc aacggttata aagaccgcga
    21901 atatatccgc ctggcattaa ttggcgagaa gatggggcac aaggtctatc tggtcattga
    21961 gaagatgtca gaaatcgcca ttgtgctgga tgaagcagaa cgtctgaatg tcgttcctcg
    22021 tctgggcgtg cgtgcacgtc tggcttcgca gggctcgggt aaatggcagt cttccggcgg
    22081 ggaaaaatcg aagttcggcc tggctgcgac tcaggtactg caactggttg aaacgctgcg
    22141 tgaagctggg cgtctcgaca gcctgcaact actgcacttc cacctcggtt cgcagatggc
    22201 gaatattcgc gatatcgcca cgggcgttcg tgaatccgcg cgtttctatg tggaactgca
    22261 caagctgggc gtcaatattc agtgcttcga cgtcggcggc ggtctgggcg tggactatga
    22321 aggcacccgt tcgcagtctg actgctccgt aaactacggc ctcaatgaat atgccaacaa
    22381 catcatctgg gcgattggtg atgcgtgtga agaaaacggt ctgccgcatc cgacggtaat
    22441 caccgaatcg ggtcgtgcgg tgactgcgca tcacaccgtg ctggtgtcta atatcatcgg
    22501 cgtggaacgt aacgaataca cggtgccgac cgcgcctgca gaagatgcgc cgcgcgcgct
    22561 gcaaagcatg tgggaaacct ggcaggagat gcacgaaccg ggaactcgcc gttctctgcg
    22621 tgaatggtta cacgacagtc agatggatct gcacgacatt catatcggct actcttccgg
    22681 cacctttagc ctgcaagaac gtgcatgggc agaacaactc tatctgagca tgtgccatga
    22741 agtgcagaag caactggatc cgcaaaaccg tgcgcatcgt ccgataatcg acgaactgca
    22801 ggaacgtatg gcggacaaaa tgtacgtcaa tttctcgctg ttccagtcaa tgccggacgc
    22861 atgggggatc gaccagttgt tcccggttct gccgctggaa gggctggatc aagtgccgga
    22921 acgtcgcgct gtgctgctgg atattacctg tgactctgac ggtgctatcg accactatat
    22981 tgatggtgat ggtattgcca cgacaatgcc aatgccggag tacgatccag agaatccgcc
    23041 gatgctcggt ttctttatgg tcggcgcata tcaggagatc ctcggcaaca tgcacaacct
    23101 gttcggtgat accgaagcgg ttgacgtgtt cgtcttccct gacggtagtg tagaagtgga
    23161 actgtctgac gaaggcgata ccgttgcgga tatgctgcaa tatgtgcagc tcgatccgaa
    23221 aacgctgtta actcagttcc gcgatcaagt gaagaaaacc gatcttgatg ctgaactgca
    23281 acaacagttc cttgaagagt tcgaggcagg tttgtacggt tatacttatc tggaagatga
    23341 gtaagtccgt attacttgaa tccgcttaat ttagcggtga taatccgcca caatttattg
    23401 tgacaaatcc aacccttcct cgtcgggcct aacgacgcgg aagggttttt ttatatcgac
    23461 tttgtaatag gagtccatcc atgagcacct taggtcatca atacgataac tcactggttt
    23521 ccaacgcctt tggtttttta cgcctgccga tgaacttcca gccgtatgac agcgatgctg
    23581 actgggtgat tactggtgtg ccgttcgata tggccacttc tggtcgtgcg gggggacgcc
    23641 acggtccggc agcgatccgt caggtttcga cgaatcttgc ctgggaacac aatcgcttcc
    23701 cgtggaattt cgacatgcgt gagcgcctga acgtcgtgga ctgcggcgat ctggtatatg
    23761 cctttggcga tgcccgtgag atgagcgaaa aactgcaggc acacgccgag aagctgctgg
    23821 ctgccggtaa gcgtatgctc tctttcggtg gtgaccactt tgttacgctg ccgctgctgc
    23881 gtgctcatgc gaagcatttc ggcaaaatgg cgctggtaca ctttgacgcc cacaccgata
    23941 cctacgcgaa cggttgtgaa tttgaccacg gcaccatgtt ctataccgcg ccgaaagaag
    24001 gtctgatcga cccgaatcat tccgtgcaga ttggtattcg taccgagttt gataaagaca
    24061 acggttttac cgtgctggac gcctgccagg tgaacgatcg cagcgtggat gacgttatcg
    24121 cccaagtgaa acagattgtg ggtgatatgc cggtttacct gacctttgat atcgactgcc
    24181 tagatcctgc tttcgcgccg ggaaccggta cgccagtgat tggcggcctg acctccgatc
    24241 gcgccattaa actggtacgc ggcctgaaag atctcaacat tgttgggatg gacgtagtgg
    24301 aagtggctcc ggcatacgat cagtcggaaa tcaccgctct ggctgcggcg acgctggcgc
    24361 tggagatgct gtatattcag gcggcgaaaa agggcgagta agcaccagat gcgatgcgca
    24421 cgggtaaaac gtgccattaa tgtcggatgc ggcgtgaacg ccttatccga cctacgttcg
    24481 gcacccgtag gccggataag atgcgccagc atcgcatccg gcaatgcgca caaggtaaca
    24541 aatgtgccat taatgtcaga tgcggcgtga acgccttatc tgacctacgt tcgacaccac
    24601 caggctttac ttaatcccat ccgcgctcat gcgatcgcga atatgctggg cgcgttcggc
    24661 ggatgccgga tggtcgtcaa acattgagct ttggcggcct tcttccagtt tcgccagttt
    24721 ttcaaagctg gtggcaagac ctgccgggct gatgccgcgt tggcgcagaa gatcgtaaga
    24781 ataatcatcg gcttctgctt cctggcgctg ggagaattgc gaattgacta atttctcgcc
    24841 cagatcacca agttgtgatt gagataaact gccgacaatc ccgcccgcag aggccgcagc
    24901 tactcgcacg gcatttgtac caagcgccac ctgcatccct ttcttcacat ggcctaacgc
    24961 cacgtgcccc atctcgtgac cgatcaccgc ttcgacttcg ttatccgtca tcatatccat
    25021 cagcccgcta tagacgcgga tacagccgtt agccattgca aaggcgttca catccttcgc
    25081 catatacact ttgtaattca ccggctgacc gttgatattg ttgcctagcg cattggcaat
    25141 agttgtcaga cgtttagcgt attcgctatt ggctggcgca atcgtcgcct tgctgtccat
    25201 ctcctgacat gcctgatcgc tcagggtttt cacctgcgca tcactcaaac tgtaagcctg
    25261 aaaagcttcc gctcctgatg agagcagtcc gttggagtcc atattctggc aaccagtcag
    25321 taccgttgcc acgctcattg ctaccaataa ggcgcgaatt ttcatgtttt ctcttccgca
    25381 caaggtcagg attattctaa aaatgcgccg tttgcaggtg aatcgacgct cagtctcagt
    25441 ataaggaaaa gcgcagcgga cgggcgagta gattgcgcaa catgcgagca tgatccagag
    25501 atttctgaag cggcaaaagg atgttccatg tacatgacgc gcggcttgcg gtaaattgtt
    25561 ggcaaatttt ccggcgtagc ccaaaacgcg ctgtcgtcaa gtcgttaagg gcgtgccctt
    25621 catcatccga tctggagtca aaatgtcctc acgtaaagag cttgccaatg ctattcgtgc
    25681 gctgagcatg gacgcagtac agaaagccaa atccggtcac ccgggtgccc ctatgggtat
    25741 ggctgacatt gccgaagtcc tgtggcgtga tttcctgaaa cacaacccgc agaatccgtc
    25801 ctgggctgac cgtgaccgct tcgtgctgtc caacggccac ggctccatgc tgatctacag
    25861 cctgctgcac ctcaccggtt acgatctgcc gatggaagaa ctgaaaaact tccgtcagct
    25921 gcactctaaa actccgggcc acccggaagt gggttacacc gctggtgtgg aaaccaccac
    25981 cggtccgctg ggtcagggta ttgccaacgc agtcggtatg gcgattgcag aaaaaacgct
    26041 ggcggcgcag tttaaccgtc cgggccacga cattgtcgac cactacacct acgccttcat
    26101 gggcgacggc tgcatgatgg aaggcatctc ccacgaagtt tgctctctgg cgggtacgct
    26161 gaagctgggt aaactgattg cgttctacga tgacaacggt atctctattg atggtcacgt
    26221 tgaaggctgg ttcaccgacg acaccgcaat gcgtttcgaa gcttacggct ggcacgttat
    26281 tcgcgacatc gacggtcatg acgcggcatc catcaaacgc gcagtagaag aagcgcgcgc
    26341 agtgactgac aaaccgtccc tgctgatgtg caaaaccatc atcggtttcg gttccccgaa
    26401 caaagccggt actcacgact cccacggtgc gccgctgggc gacgctgaaa ttgccctgac
    26461 ccgcgaacag ctgggctgga aatatgcacc gttcgaaatc ccgtctgaaa tctatgctca
    26521 gtgggatgcg aaagaagcag gccaggcgaa agaatccgca tggaacgaga aattcgctgc
    26581 ttacgcgaaa gcttatccgc aggaagccgc tgaatttacc cgccgtatga aaggcgaaat
    26641 gccgtctgac ttcgacgcga aagcaaaaga gttcattgct aaactgcagg ctaacccggc
    26701 gaaaatcgcc agccgtaaag cgtctcagaa tgctatcgaa gctttcggtc cgctgttgcc
    26761 ggaattcctc ggcggttctg ctgacctggc accgtctaac ctgaccctgt ggtctggttc
    26821 taaagcaatc aacgaagatg ctgcgggtaa ctacatccac tacggtgttc gcgagttcgg
    26881 tatgaccgcg attgctaacg gtatctccct gcacggtggt ttcctgccgt acacctccac
    26941 cttcctgatg ttcgtggaat acgcacgtaa cgccgtacgt atggctgcgc tgatgaaaca
    27001 gcgtcaggtg atggtttaca cccacgactc catcggtctg ggcgaagatg gcccgactca
    27061 ccagccggta gagcaggtcg cttctctgcg cgtaaccccg aacatgtcta catggcgtcc
    27121 gtgtgaccag gttgaatccg cggtcgcgtg gaaatacggt gttgagcgtc aggacggccc
    27181 gaccgcactg atcctctccc gtcagaacct ggcgcagcag gaacgaactg aagagcaact
    27241 ggcaaacatc gcgcgcggtg gttatgtgct gaaagactgc gccggtcagc cggaactgat
    27301 tttcatcgct accggttcag aagttgaact ggctgttgct gcctacgaaa aactgactgc
    27361 cgaaggcgtg aaagcgcgcg tggtgtccat gccgtctacc gacgcatttg acaagcagga
    27421 tgctgcttac cgtgaatccg tactgccgaa agcggttact gcacgcgttg cggtagaagc
    27481 gggtattgct gactactggt acaagtatgt tggcctgaat ggcgctatca tcggtatgac
    27541 caccttcggt gagtcagctc cggcagagtt gctattcgaa gagttcggct tcaccgtcga
    27601 caacgtcgtt gcgaaagcaa aagaactgct gtaattagca tttcgggtaa aaaggtcgct
    27661 tcggcgacct tttttattac cttgatatgt ccgtttgcgg acaagcaata gataaagcgt
    27721 gttgcagatc acaaatattt atatgcaata aatatcaatt atgtaatatg catcacgata
    27781 tgcgtattga catttgttgt tatatctata actcaatgtt atataagaaa ttaataattc
    27841 actgttttca aaacaccggt ttccctgctc aattgctttc attaaaccgc acgagtattt
    27901 tatttttacc gccggacggt accctaaaaa taaaataagg ataatttatg cggcttagtg
    27961 actattttcc agaatcatca atctcagtta tacattcagc aaaagattgg caggaagcta
    28021 tcgatttctc gatggtatca ttgctggata aaaactatat cagcgagaat tacattcagg
    28081 caattaaaga ttccaccatt aacaatggcc cttattatat tctcgcacca ggcgtggcaa
    28141 tgcctcatgc gcgaccggaa tgtggggcgc ttaaaaccgg gatgtctttg acattacttg
    28201 aacaaggtgt ttattttccg gggaatgacg aaccaattaa attactcatc ggactctctg
    28261 ctgccgatgc cgattcgcac attggcgcta ttcaggcgtt aagtgaatta ctgtgcgaag
    28321 aagaaatact cgaacaactc ttaacagcat catcagaaaa acaattagcg gacattatca
    28381 gccacggata attactctct cctctacagg ataatattat ggaaaacaag tctgctcgtg
    28441 caaaggtcca ggcttttggg ggctttttga ctgcaatggt catccccaat attggtgctt
    28501 ttattgcctg gggttttatt actgcgttat ttattcccac cggttggctg cctaacgaac
    28561 atttcgccaa aattgtcggc ccgatgatta cctatttatt gcccgtgatg attggttcta
    28621 caggtggtca tctggtcggc ggtaaacgcg gggcggtcat gggcggaata ggtactattg
    28681 gtgtgatcgt tggcgcagag atcccgatgt tccttggctc aatgatcatg gggccgctcg
    28741 gtgggttggt cataaaatat gtcgataagg cactggaaaa acgcatacct gccggttttg
    28801 agatggttat caataacttc tcattaggta tcgcggggat gctcctttgt ctgctgggtt
    28861 ttgaagtgat cggcccggcg gtgttaattg ccaatacttt cgtcaaagag tgtatcgagg
    28921 cgctggtaca tgcgggttat ctgcctctgc tgtcagtcat caatgaaccg gcgaaagtgc
    28981 ttttccttaa taatgcgatc gatcagggcg tctattatcc gctgggaatg caacaggctt
    29041 cggttaacgg taaatccatc ttctttatgg tggcctctaa cccaggtccg ggcctggggc
    29101 tgctgctggc gtttaccttg tttggtaaag ggatgagtaa acgttctgcg cccggggcga
    29161 tgattattca cttcctcggt gggatccacg aactgtattt cccatatgtg ctgatgaagc
    29221 cgctgactat tattgccatg attgcgggcg gtatgtctgg tacctggatg tttaacttac
    29281 tggacggtgg tctggtggct ggcccaagtc tggggtctat ctttgcttac ctggcactga
    29341 cgccgaaagg ctcgttcctg gcgacaattg ccggtgttac ggtaggtacc ctggtgtcct
    29401 ttgctattac ttcgctgata ctgaagatgg aaaaaacggt ggaaacggag agcgaagatg
    29461 agtttgctca gtcagccaat gcggttaagg cgatgaaaca agagggtgca ttctcgttaa
    29521 gcagggttaa gcgtatcgcc tttgtttgcg atacggggat gggctccagt gcgatgggcg
    29581 cgaccacctt ccgtaaacgc ctggaaaaag cggggctggc aatagaagta aaacattacg
    29641 cgatagaaaa cgtgcctgcg gatgccgata tcgtcgtcac tcacgccagt ctggaagggc
    29701 gcgtgaaacg tgtgacggat aaaccgctga tattgattaa taactatatt ggcgatccaa
    29761 aactcgacac tttatttaat caattaaccg ccgaacataa acactgatta cagaggtaaa
    29821 aatgaaaacc aaagttgctg ctatttatgg caagcgggat gtccgtctgc gcgaatttga
    29881 actgccagaa attaccgata atgaactgtt agtgagtgta atttctgaca gcgtctgttt
    29941 atcgacctgg aaagcggcgt tactcggtag tgaacataaa cgcgtacccg acgatttaga
    30001 aaatcatccg gtcattaccg ggcatgaatg tgccggggtt attgtcgaag tgggtaaaaa
    30061 tctcactggc aaatataaaa aaggccagcg ttttgtattg caaccggcga tggggttacc
    30121 aagcggatat tcagcaggct acagctacga atattttggc ggtaacgcca cctatatgat
    30181 tattcccgaa atcgccatta atttgggctg cgtattaccg tatcacggct cttattttgc
    30241 tgcggcgtcg ctggtagagc ctatgtgctg cattattggt gcttatcatg ccaattatca
    30301 caccacgcaa tatgtttatg agcatcgcat gggcgtcaaa cctggcggca atattgcact
    30361 gctggcgtgt gcgggtccga tgggcattgg cgctatcgat tacgccatta acggtggcat
    30421 acaaccgtca cgggtggtgg tggtcgatat cgacgacaaa cgcctggcgc aggtgcagaa
    30481 gctgctgccg gtggatctgg cggccagtaa aggcattgag ctggtgtatg tgaataccaa
    30541 agggatgagc gatccggtcc agacgctgcg ggcgctgaca ggagatgtcg ggttcgatga
    30601 catttttgtt tatgcggcgg tgcctgctgt cgttgagatg gctgacgaat tactggcgga
    30661 agatggctgt ctgaacttct ttgccgggcc gacggataaa aacttcaaag tgccgtttaa
    30721 tttctacaac gtccattaca acagcacgca cgtagtcggt acatccggcg gttcaacgga
    30781 cgacatgaaa gaggcgattg cgttaagcgc cacggggcag ttgcagccgt cctttatggt
    30841 cacgcatatc ggtgggctgg atgcggtgcc agataccgtg ctcaatctgc cggatatccc
    30901 tggcggtaaa aaactcattt ataacggcgt gaccatgccg ctcactgcca ttgccgattt
    30961 tgccgaaaaa ggcaaaaccg atccgctatt taaagagttg gcgcggctgg ttgaggaaac
    31021 gcacggcatc tggaatgaac aggccgagaa atatctgctg gcacaatttg gcattgatat
    31081 cggggaggcc gcgcaatgat gtccctggcg tggccattat ttcgcgttac ggaacaggca
    31141 gcgttggctg cctggccgca aaccggatgt ggcgacaaaa ataaaattga tggcctggcg
    31201 gtcaccgcga tgcgccaggc attaaacgac gtcgcttttc gtggacgagt ggttatcggc
    31261 gaaggagaaa ttgaccatgc gccgatgctg tggattggcg aagaggtggg caaaggcgat
    31321 gggccagaag tcgatatcgc ggttgatccc attgaaggca cgcggatggt ggcgatgggg
    31381 cagagcaatg cgctggcggt catggctttc gcccctcggg atagcctgtt acatgcgccc
    31441 gatatgtata tgaaaaagct ggttgttaat cgactggctg ccggggtgat tgatctgtct
    31501 ttgcctctgg cggacaactt gcgtaacgtg gcgcgggcgt taggtaaacc gctggacaag
    31561 ctgcgcatgg ttacgctgga taaaccgcgc ctgagtgccg cgattgagga agcaacacag
    31621 ctgggcgtga aggtttttgc cctgccggat ggcgatgtcg ccgccagcgt gctgacctgc
    31681 tggcaggata atccttacga tgtgatgtac accatcggcg gtgcgccaga aggcgtgatt
    31741 tctgcctgtg ccgttaaagc attaggcggg gatatgcagg ccgagctgat tgacttttgt
    31801 caggctaaag gggattacac ggaaaatcgg cagattgcag agcaggagag caagcgttgc
    31861 aaggcaatgg gcgttgacgt taaccgtgtg tactcgctcg atgaactggt aagggggaac
    31921 gatatcctct ttagcgctac gggcgtgacg ggcggcgagt tggtgaacgg tatccaacag
    31981 acggcgaatg gggtgcggac gcagacatta ctgatcggcg gcgcggacca aacgtgtaat
    32041 ataatagact ccctgcattg atggtgatct atcggaagac caatggcgaa gctgacagaa
    32101 gatgatgtgc ttgagcaact ggatgcacag gacaatttat tttcatttat gaaaactgcg
    32161 cattctattt tgctccaggg gatacgccag tttctgccgt cgctgtttgt cgataacgat
    32221 gaagagatcg tggaatatgc agtgaagccg ttactcgccc aaagcggccc gcttgacgat
    32281 attgatgttg cgctgcgttt gatttatgcg ctggggaaaa tggataaatg gctgtacgcc
    32341 gatatcacgc atttttccca gttctggcat tacctgaacg aacaggatga aacgcccgga
    32401 tttgccgatg acatgacctg ggattttatc agcaacgtca atagcataac ccgcaatgcg
    32461 atgctctatg acgcgttgaa agcgatgaag ttcgccgatt tcgccgtctg gtcagaggcg
    32521 cgttttagcg ggatggtcaa aacggcgctg acgctggcag taacgacaat cttaaaggaa
    32581 ttaacgccgt gaaaattgaa ttaacggtga atgggctgaa gatacaggca cagtaccaga
    32641 acgaagagat tgaaaatgta cataaaccgt tgctgcgtat gctggcggcc ttacagacgg
    32701 taaatccgca gcggcgtacg gtggtttttc tttgcgctcc gccggggacg ggtaaatcta
    32761 ccctaaccac cttctgggaa tatctcgcgc aacaagaccc ggagctgccc gctattcaga
    32821 cgctcccgat ggatggtttt caccattaca atagctggct ggacgcgcat caattgcgcc
    32881 ccttcaaagg cgcaccagag acatttgacg ttgcgaaact ggcggaaaat ctgcgccagg
    32941 tggtggaagg ggattgtacg tggccgcagt acgatcgaca aaagcatgat cctgttgaag
    33001 atgcgttgca cgttaccgca ccactcgtca tcgtcgaagg aaactggttg ttgctggacg
    33061 acgagaagtg gtgccagctt gcgcaatttt gtgatttctc gatctttatc aacgcgccag
    33121 ctactgcgtt gcgggagcgt ctggtggggc gcaagctggc gggaggggta tcgctggcag
    33181 atgcagaagc cttttatgac cgtaccgacg ggccgaatgt tcgccgggtg ctggaagaga
    33241 gtctgcctgc aaatttgacc ttaatgatga ccgctacggg tgaatatcgt ctggtggatt
    33301 aacgttcctg attacaccat cttccagtcg caccacacga gagaaatgac gccgggtaaa
    33361 tgcggcgtca tgactgattg ctaccacgct cgtgcctcgc tggcggcact tctccagcca
    33421 gctttcaaac acgctcaacc aattttcgtc aaaatcccga ctcggctcgt ccagcagtaa
    33481 tagtggcgga gagagtgctt ccaggcaggc gacggcgacc atccgtcgct gcgcactatg
    33541 cagatccaac gggtgcgagt tggcagtgtc tgctaactga caacattgca gggcagcatg
    33601 ggtacgttga gtgatttcat ccgccgggca tttttgcagt ttcaaaccaa atgcgatctc
    33661 gtcagcgacg gtgctgtgaa aaagttggtt ttccgcttcc tgaaacagta cgccaacctt
    33721 tgccgcgcgc tggcggtttt tcaggttttt catcgcctgt tgttgcagca taaccgtacc
    33781 ggctgtgggc gtaagaagac ctgccattac tcgtaacagc gtcgatttcc ctgcgccatt
    33841 gtcaccggtc agcgccaacc attcaccttg tttgagttgc agagaaatgt cgcaaagaca
    33901 atccgttgcc gcgccaggcc agcgataaga tatctgattt aacgttaaca tagtggctgg
    33961 agcgctccgt tttgcaattg ccacgcttgc tggcagtagt tcaggaaagg tgtgcgatga
    34021 cgttcaaaaa gaataatcag cgaaccacgt tcaaacgccc agtgttgtaa gcgctgcaac
    34081 agcatttcgc tggcctgcgg cgtcaggcgg ctaaaggctt catcaagaat taagagcttt
    34141 ggctgcatcg cgatggcgca ggcgatgacc acgcgctgtg tctctccacc ggagagtgtt
    34201 gccgggtgac gatggcgcaa cggctggcac tcggtcagag tcagggcggc atcaatccgc
    34261 gccataatct ctgcttcagc aagacacaga ttttctggcc caaaggcaac ttcttcttcc
    34321 acgctaaagg tacagccgga gagctgcaaa tagggcgatt gctggacgag ctggatggtg
    34381 gcggattgtt cattaagcgg gagatgccca atgggcgtac cgaggagcgt ccccgtgcct
    34441 gttatctctc ctggcagaaa gtccggatac cagcccgcca tcagctgtgc cagcgtactt
    34501 ttgccactgc cgttgtcacc aaagatggcg accatccccg gtttgacata gtgaaagtca
    34561 taacagaacg gaggatgcgt tgagtgtgtc gggcaataac ggaactgttc taacgtaacc
    34621 atatccacgc tccgaattct gtcagcatca gcaagatcat ggtgtaacgc gctacgcgtt
    34681 gtagggtgct atcggctggt gcccacaacg ttgttcgtcg gttgtggatc cgaaatgccc
    34741 gcatatccag cgccgcacca cggacagcca gatcgttaag cgcattgtgt gttagcggaa
    34801 taattaacgc gggcatcgca cgtaaacgct gataccagcc ctcatccagc ggcacgccgc
    34861 gggcacgctg cgcttcatga ataatcgcta attgccgttt taactgctca acgaccaata
    34921 acggcccggc aaacagataa gcgacgcctg gcggcaggcg agaagcaaac agagcgcgaa
    34981 taaaacattg cacggggacg tactgcatcc acaactgtga ggttgaaaca atcgccagaa
    35041 tccgcaacca gagcgtaata gcatgcgacc accgttctgg cgagcgtggc gtgccgctta
    35101 gccattctgg tagccagcca ccgtgtacca gccacagccc ggctcccagc gagaacatca
    35161 gccagacgac atattttgcc cgccgccgcg tggcctttag cgcaataagg cagaaaaagg
    35221 tcgccgcgct atagatcggc aaaatggtct gcgcgggcag gataagcgtg gtgcaggccg
    35281 ccaatgccca cagtattaat gaggtaaatg gatgcattta gcgcacggca gccattgccg
    35341 gaaaatggcg cgtggtgcga atcggcagct ggcgcagtaa aagccagaca atcaccgctg
    35401 taagaatttt atcgaccagg ttcgcgccta tcaccgtaat cgccaccgac tccaccaggt
    35461 tttgccccat cgagtgcatc caggcaacga acaaatcggc tccgctaccg gtgacaccgc
    35521 cgaacagcgc agtacgcagc ggtacagcga cgaccgttac cgcaagcgta ataatcacgc
    35581 cgctgacaac gacttttggc agggtgcgaa accaacccgc tcgcgccagc caaccggcga
    35641 ccaggccgat gaccatcgcg acgggtgcaa atgcggcggc gatcgggtcg gtcagcaatc
    35701 cccagagtaa attggtcagt aagccggtca gcattccgat caccggacca agcagtaccg
    35761 cgctaataag tgtgccgatg gagtcgagaa atataggtaa tttgaccatg ctggccagtt
    35821 gcccgccgat catattgatg gcgatagata taacgatcaa caccagtgcc tgactggaaa
    35881 agtggctacg cgccataaga aaacctcttt gaaagtagtt atttaacggg ggaaatatgc
    35941 aactcgtcgt aaccagaaag cgcgcaaggt tggcggctaa acgccagttc ctcaaggcta
    36001 ccgatcacca gttcaagcgt ggtccgcacg gtacagccgg gcatcatatg accgccaagc
    36061 atcgtgccgt gtggatcgga aacgcagaga tgaagatgct caccactctg ttccagcgtg
    36121 ccgtttagtg agatcacttc gaatgtaccg ttcaggagcg tggtgccttc ttgcctggca
    36181 tagcgcaagg cgacgtcagt caggctaccg gtacagcctg caatccaggc ggcatgaagt
    36241 tgatgctgct gtgcaaaagc gtgtagctgg gaaaaaactt cctgcccggg gaggaggcgc
    36301 agtgcgtaaa agcgtgcgga ggaagaatat gggtagggca atgtcatcat tggagtactc
    36361 cgaaactcgg acgtattgcg gataatatcg acgctgcatc agtcatattc tgatataagt
    36421 cagggtttgt gacggcacaa cgaaaagatt ttgctacgct tattcgtgat tctgcaaagt
    36481 ggaataaatt ctacaggggc ggggctgaaa acgtgacgta agtcagataa ttgctttctt
    36541 tgactggaca atcattcctt ttattccacg tttcgcttat cctagctgaa gcgtttcagt
    36601 cgattaaatg ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa ggtttccccg
    36661 gacgatttgc tggattactc tgtctggcaa atttgctcag ggaaaacctt gcaggagatc
    36721 tatgaccgta cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg tggttcgtgc
    36781 tttgtatgaa tccggacgcc gggcggaaat taccgtggtg gcaatcaacg aactggcgga
    36841 tgctgcgggc atggcgcatt tgttgaaata tgacaccagc catggccgtt ttgcatggga
    36901 agtacgacag gaacgcgatc aactttttgt tggtgatgac gccatccgcg tattgcatga
    36961 acgttcactg caatcgctcc cctggcgtga acttggcgtt gatgtagtcc tcgactgcac
    37021 cggcgtatat ggctcccgcg agcatggcga agcgcatatt gccgccgggg ccaaaaaagt
    37081 gctcttttca catcctggca gtaacgatct cgacgcgacc gttgtttacg gcgtcaatca
    37141 ggatcaactt cgtgcggaac accgcatcgt ttctaacgct tcctgtacca cgaattgcat
    37201 aattcccgtc atcaaattgt tagatgatgc gtacggtatt gagtccggca ctgtgaccac
    37261 aattcactcc gccatgcacg atcaacaggt tattgatgca taccatcctg acctgcgtcg
    37321 cacccgggca gccagccagt cgatcattcc ggtcgatact aaactggccg ccggtatcac
    37381 acgatttttt ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg tgccaaccat
    37441 aaatgtgacg gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag ccaatgaagt
    37501 caacctgttg ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg actatacgga
    37561 attgccgttg gtctctgtag attttaacca cgatccgcac agtgccattg tcgatggcac
    37621 ccaaacccgg gtcagtggcg cacacctgat caaaacgttg gtctggtgcg ataacgaatg
    37681 gggctttgct aaccgaatgc tcgacacgac gttagctatg gctactgttg ctttcaggta
    37741 agacgcaagc agcgtctgca aaacttttag aatcaacgag aggattcacc atgtctgtaa
    37801 ttaagatgac cgatctggat cttgctggga aacgtgtatt tatccgtgcg gatctgaacg
    37861 taccagtaaa agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct ctgccgacca
    37921 tcgaactggc cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg ggtcgtccta
    37981 ccgaaggcga gtacaacgaa gaattctctc tgctgccggt tgttaactac ctgaaagaca
    38041 aactgtctaa cccggttcgt ctggttaaag attacctcga cggcgttgac gttgctgaag
    38101 gtgaactggt tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa gacgacgaaa
    38161 ccctgtccaa aaaatacgct gcactgtgtg acgtgttcgt aatggacgca ttcggtactg
    38221 ctcaccgcgc gcaggcttct actcacggta tcggtaaatt cgctgacgtt gcgtgcgcag
    38281 gcccgctgct ggcagctgaa ctggacgcgc tgggtaaagc actgaaagaa cctgctcgcc
    38341 cgatggtggc tatcgttggt ggttctaaag tatctaccaa actgaccgtt ttggactccc
    38401 tgtctaaaat cgctgaccag ctgattgttg gtggtggtat cgctaacacc tttatcgcgg
    38461 cacaaggcca cgatgtgggt aaatccctgt acgaagctga cctggttgac gaagctaaac
    38521 gtctgctgac cacctgcaac atcccggttc cgtctgatgt tcgcgtagca accgagttct
    38581 ctgaaactgc accggctacc ctgaaatctg ttaacgatgt gaaagctgac gagcagatcc
    38641 tggatatcgg tgatgcttcc gctcaggaac tggctgaaat cctgaagaat gcgaaaacca
    38701 ttctgtggaa cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa ggtactgaaa
    38761 tcgtggctaa cgctatcgca gacagcgaag cgttctccat cgctggcggc ggcgacactc
    38821 tggcagcaat cgacctgttc ggcattgctg acaaaatctc ctacatctcc actggcggcg
    38881 gcgcattcct cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg ctcgaagagc
    38941 gcgctaagaa gtaaaaaatc acagggcagg gaaacctgcc cttgtttcag cgcgctttta
    39001 gagcacgcat tttttcatac tctaaataat tcgagttgca ggaaggcgac aagagagtga
    39061 atccccagga gcttacataa gtaagtgact ggggtgagcg aacgcagacg cagcacatgc
    39121 aacttgaagt atgatgagta taaggcccga cgatacagga caagagacat gtctaagatt
    39181 tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt tttccaggta
    39241 gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga ctccatcaac
    39301 gccgtactgg aaactgctgc taaagttaaa gcgccggtta tcgttcagtt ctccaacggt
    39361 ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg tgctgctatc
    39421 ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta tggtgttccg
    39481 gttatcctgc acactgacca ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg
    39541 gacgcgggtg aaaaacactt cgcagctacc ggtaagccgc tgttctcttc tcacatgatc
    39601 gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata cctggagcgt
    39661 atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg tgaagaagac
    39721 ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga agacgttgat
    39781 tacgcataca ccgagctgag caaaatcagc ccgcgtttca ccatcgctgc gtccttcggt
    39841 aacgtacacg gtgtttacaa gccgggtaac gtggttctga ctccgaccat cctgcgtgat
    39901 tctcaggaat atgtttctaa gaaacacaac ctgccgcaca acagcctgag cttcgtattc
    39961 cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg
    40021 aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct gaactactac
    40081 aaagcgaacg aagcttatct gcagggtcag ctgggtaacc cgaaaggcga agatcagccg
    40141 aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc gatgatcgct
    40201 cgtctggaaa aagcattcca ggaactgaac gcgatcgacg ttctgtaaga tattcctttc
    40261 tgcttatctc aaggcccgct ctgcgggtct ttttttcgcc aaaagcaaaa ttgcctgatg
    40321 cgctacgctt atcaggccta cacgtatcct gcaatatatt gaatttgcag gtttttgtag
    40381 gccggataag gcgttcacgc cgcatccggc atgacaaagc gcacattgat atctgtctga
    40441 gccccgctct gcggggattt tttcgccaaa agcaaggtga ttcagatgag aaatctgtga
    40501 cctatttggc aaaattatgc tttattgttt acccttgtca gactgcccgt cataaggcgg
    40561 cggagtgtat ttctccattt tgagtcagtt gaaaaggaat attgaatgga agatttgaat
    40621 gttgtcgata gcataaacgg cgcgggaagc tggctggtag ctaaccaggc gctgctgcta
    40681 agttatgcag taaacatcgt ggcggcactc gcgatcatca tcgttggttt gattatcgcg
    40741 cggatgattt ccaacgcggt gaatcgcctg atgatctccc gtaaaatcga tgccactgtt
    40801 gctgattttc tttctgcatt agtccgttac ggtattatcg cctttacgct aatcgctgcc
    40861 ctgggacgcg tgggtgtaca aacagcgtca gtcattgctg tactcggtgc cgcaggctta
    40921 gctgttggtc tggctttgca ggggtcactt tctaacctgg ccgctggcgt gttacttgtc
    40981 atgttccgac cgttccgtgc cggagaatat gttgacctcg gcggcgtagc cggtactgtg
    41041 ctgagtgtgc agattttctc caccaccatg cgtactgcag acggtaaaat tatcgttatt
    41101 ccgaacggta aaattattgc cggaaatatt attaacttct cccgcgagcc agttcgccgt
    41161 aacgaattta ttattggcgt ggcgtatgat tccgatatcg atcaggttaa gcagatcctg
    41221 accaatatta tccagtctga agatcgcatt ttgaaagatc gcgaaatgac tgtgcgcctg
    41281 aacgaacttg gtgcatcgtc gattaatttc gtggtccgcg tctggagcaa cagcggcgat
    41341 ctgcaaaacg tgtactggga tgtgctggag cgtattaaac gtgaatttga tgccgccggt
    41401 atcagcttcc cgtacccgca aatggatgtg aactttaagc gggtgaaaga agacaaagct
    41461 gcgtaatcaa cgctgaggcc agataatact ctctggcctc tctcttatta gttattctga
    41521 ttgccaatta atattatcaa tttccgctaa taacaatccc gcgatatagt ctctgcatca
    41581 gatacttaat tcggaatatc caacgtgttt tcttattact ttcaaggtct tg
//
LOCUS       DAJTNN010000011        40625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTNN010000011 DAJTNN010000000
VERSION     DAJTNN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.011"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(265..1122)
                     /gene="murI"
                     /locus_tag="OO150_000570"
     CDS             complement(265..1122)
                     /gene="murI"
                     /locus_tag="OO150_000570"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5514822.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1067..2915)
                     /gene="btuB"
                     /locus_tag="OO150_000571"
                     /pseudo
     CDS             complement(1067..2915)
                     /gene="btuB"
                     /locus_tag="OO150_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2969..3159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3273..4373
                     /gene="trmA"
                     /locus_tag="OO150_000572"
     CDS             3273..4373
                     /gene="trmA"
                     /locus_tag="OO150_000572"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5514823.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4413..4772)
                     /locus_tag="OO150_000573"
     CDS             complement(4413..4772)
                     /locus_tag="OO150_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5514824.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4772..5422)
                     /gene="fabR"
                     /locus_tag="OO150_000574"
     CDS             complement(4772..5422)
                     /gene="fabR"
                     /locus_tag="OO150_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5514825.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5743..7143
                     /gene="sthA"
                     /locus_tag="OO150_000575"
     CDS             5743..7143
                     /gene="sthA"
                     /locus_tag="OO150_000575"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5514826.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7126..8043)
                     /gene="oxyR"
                     /locus_tag="OO150_000576"
     CDS             complement(7126..8043)
                     /gene="oxyR"
                     /locus_tag="OO150_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5514827.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8310..9683)
                     /gene="argH"
                     /locus_tag="OO150_000577"
     CDS             complement(8310..9683)
                     /gene="argH"
                     /locus_tag="OO150_000577"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5514828.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRLAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDAFDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9744..10520)
                     /gene="argB"
                     /locus_tag="OO150_000578"
     CDS             complement(9744..10520)
                     /gene="argB"
                     /locus_tag="OO150_000578"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5514829.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10528..11532)
                     /gene="argC"
                     /locus_tag="OO150_000579"
     CDS             complement(10528..11532)
                     /gene="argC"
                     /locus_tag="OO150_000579"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5514830.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11686..12837
                     /gene="argE"
                     /locus_tag="OO150_000580"
     CDS             11686..12837
                     /gene="argE"
                     /locus_tag="OO150_000580"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5514831.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDIDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVIERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            13189..15840
                     /gene="ppc"
                     /locus_tag="OO150_000581"
     CDS             13189..15840
                     /gene="ppc"
                     /locus_tag="OO150_000581"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5514832.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEAPPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYKSWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYL
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            16023..17756
                     /locus_tag="OO150_000582"
     CDS             16023..17756
                     /locus_tag="OO150_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5514833.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17971..18822
                     /locus_tag="OO150_000583"
     CDS             17971..18822
                     /locus_tag="OO150_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5514834.1"
                     /translation="MYHDISYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18809..19150)
                     /locus_tag="OO150_000584"
     CDS             complement(18809..19150)
                     /locus_tag="OO150_000584"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5514835.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19152..20030)
                     /locus_tag="OO150_000585"
     CDS             complement(19152..20030)
                     /locus_tag="OO150_000585"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5514836.1"
                     /translation="MTSSAGQRISCNVVETRLNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19996..22293)
                     /locus_tag="OO150_000586"
     CDS             complement(19996..22293)
                     /locus_tag="OO150_000586"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5514837.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTPGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22344..22664)
                     /locus_tag="OO150_000587"
     CDS             complement(22344..22664)
                     /locus_tag="OO150_000587"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5514838.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22679..23758)
                     /locus_tag="OO150_000588"
     CDS             complement(22679..23758)
                     /locus_tag="OO150_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5514839.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24067..26568
                     /gene="ptsP"
                     /locus_tag="OO150_000589"
     CDS             24067..26568
                     /gene="ptsP"
                     /locus_tag="OO150_000589"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5514840.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSSILEAHRSLAGDTSLRQHLLTGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMTQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26580..27242
                     /gene="fsa"
                     /locus_tag="OO150_000590"
     CDS             26580..27242
                     /gene="fsa"
                     /locus_tag="OO150_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5514841.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27253..28356
                     /gene="gldA"
                     /locus_tag="OO150_000591"
     CDS             27253..28356
                     /gene="gldA"
                     /locus_tag="OO150_000591"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR5514842.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAAFSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28630..29247
                     /locus_tag="OO150_000592"
     CDS             28630..29247
                     /locus_tag="OO150_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR5514843.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29274..30179)
                     /gene="yijE"
                     /locus_tag="OO150_000593"
     CDS             complement(29274..30179)
                     /gene="yijE"
                     /locus_tag="OO150_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5514844.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMHPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(30273..32453)
                     /gene="katG"
                     /locus_tag="OO150_000594"
     CDS             complement(30273..32453)
                     /gene="katG"
                     /locus_tag="OO150_000594"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5514845.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     SSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32782..33672)
                     /gene="metF"
                     /locus_tag="OO150_000595"
     CDS             complement(32782..33672)
                     /gene="metF"
                     /locus_tag="OO150_000595"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5514846.1"
                     /translation="MSFFHASQRDALNQRLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(34021..36453)
                     /gene="metL"
                     /locus_tag="OO150_000596"
     CDS             complement(34021..36453)
                     /gene="metL"
                     /locus_tag="OO150_000596"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5514847.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36456..37616)
                     /gene="metB"
                     /locus_tag="OO150_000597"
     CDS             complement(36456..37616)
                     /gene="metB"
                     /locus_tag="OO150_000597"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5514848.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37893..38210
                     /gene="metJ"
                     /locus_tag="OO150_000598"
     CDS             37893..38210
                     /gene="metJ"
                     /locus_tag="OO150_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5514849.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38394..39002
                     /locus_tag="OO150_000599"
     CDS             38394..39002
                     /locus_tag="OO150_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5514850.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            39147..39822
                     /locus_tag="OO150_000600"
                     /pseudo
     CDS             39147..39822
                     /locus_tag="OO150_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39994..40266)
                     /locus_tag="OO150_000601"
     CDS             complement(39994..40266)
                     /locus_tag="OO150_000601"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514851.1"
                     /translation="MELQLSDKGVIKMKAIQCCYFLALVSSNLMAGVMGNDNIFLSNS
                     DSAALYCVAKIFPDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(40232..40625)
                     /locus_tag="OO150_000602"
                     /pseudo
     CDS             complement(40232..40625)
                     /locus_tag="OO150_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
BASE COUNT         9991 a        11161 c        10102 g         9371 t
ORIGIN      
        1 cccacacaga ttgtctgata aattgttaaa gagcagtgcc gcttcgcttt ttctcagcgg
       61 cgcggggtgt gcataatacg ctttcccgct acagagtcaa gcatttattt ttgctttctc
      121 tgtcggaatt ctcaggagaa ccccgctgac ccggcggcgt gtttgccgtt gttccgtgtc
      181 agtggtggcg cattataggg agttattccg gcctgacaag aggaaattta aaataatttt
      241 ctgaccgcgc aacattcaac caaatcagcc taaaactgcc agtttttcga gcgtttcgaa
      301 gccgtaacgc tgtaaaacgg gcaataattg ttcagcttct ggcgtcattg ccatacaaaa
      361 ggcaatattc gcatcggcag attttgcatc cggggcttca tgttctaaca accaggccgt
      421 tcggcgagca atcgctgcgc cagaatccac tagccgggtt ccctctggca gcacttgtaa
      481 cagttcttct tgtagtagag ggaaatgggt gcaacccaat acaacggtat ctggcggctc
      541 tttcattctt aaccacgggc gtaggatacg ttttagtgca tccagagaaa catcttcgcc
      601 atgtagcttc gcttcagcca actcaaccat ctctgccgag cccagcattt ctatctggca
      661 ttcattagcg aaacgcgcga tcagctcatg agtataagaa cgtttaactg ttccgcgggt
      721 tgccagtaat ccgacaatgc catttgccgt cagacgtgca gcaggtttaa tcgccggcac
      781 gacaccaaca accgggaagt cgaacttttc gcgtaatgca ggaagtgaaa cggtactggc
      841 agtgttgcaa gcgaccacag ccagcgcaag gggataacgt tcttgcaccg cggtgacaat
      901 tgccaccact cgctcaacaa taaacgcttc gcttttttcg ccatacggga aagcgacgtt
      961 atcgaaagca taaatgtaat ggagatccgg taagagatgc cggatctcgt catagaccga
     1021 caacccaccg acgccggagt caaacaccag cacggtggga cgtggttcag aaggtgtagc
     1081 tgccagacaa ggtgtattcc cgtcctgcag tttggtagcc atagactgtc tcataatctt
     1141 tgtcgaacag gttggctatt ttaccacgaa ctgtcaggtg agaggtgacc ggatacgcaa
     1201 ccgcaagatc ccacaagctc acaccgccca ttttaacggt ttgataagga taagatgagt
     1261 aatccttatc atagcgagtg cctaaatact gataagtaat accccagtcg aagtcataca
     1321 actgccagtc gagctggtat ttcacctgct gtttagcacg gcgtaacaac ggcgtgtcgg
     1381 taatcgcatt gcgcgcatcg acataatcat aactcacagt atgcgtcagt ggtccggtat
     1441 caaaattggc ggtcgcctcg acgcccttaa tccgcgcttt cccttcgttg taatatttca
     1501 gggtgtgatc atcataatcg atcaagtcac tgacatcgtt acgatatccg gaaatacgcc
     1561 agttcacccc agcggttaag ccttcaaacg cgccttccca ctgtttgctt ttctccgggt
     1621 ccagattcgg atttccgtag aagccataca gttgccccag atttggtgcc ttataagatg
     1681 tcccgtagga agcaatgaag cgataacctt cgatgaattc ccaaccggcg ctggtttgcc
     1741 aggttccatg acgaccaaac tgtgagttat cgtcactgcg tgctgcgcct tcaaaggtaa
     1801 aatcgccgac ttgttgcagc ccggtcagat agatgccggt attacgttga tcatatccat
     1861 cctcaacata acctgtaccc ggcgtcgtag tctgtttctg ccagtcgaca cccgcaccaa
     1921 tactaccgtg accaacgatg acattgtttg cccactggac ggtgtattgc ttcatctcat
     1981 cgagcgtcgc cgacgaatca taacgaccat aatggggatc gtagttgtaa tctttgctat
     2041 ggctatagct ggtaatgagt tgtgatttaa tcagttcgcc gttatagcgc agcccggcgt
     2101 cccaactttg gctatagagt ttacggatat cgagcaacgg tgaaccggga gaataatacg
     2161 cgtcataatt ggtacggtta tcatagccat agccgcgcac aaagccgctc caggcatcag
     2221 taaagttatg ctccagcgcg ccataaagcg ttttacttaa aaaaacatcg ttatctgtct
     2281 gcgcttgcgt tccggtatta ccataggcaa caacatcata accatgagta tgggcataat
     2341 cgcccaacag cgttacccgt gtcttatccc ccagttgttg ctgcgtagag acatcatagt
     2401 tctgataact attgcttccc caccctgctg aaatttccgt tccggggtca tcgcgcgtcg
     2461 tgatgatatt caccaccccg cctattgcat cggaaccata aacagcggag cgcggcccac
     2521 ggatatattc aacacgctgg acaagcgcac gcaataggga actggctaag gtcggcagaa
     2581 ccactcaccc ccgccagatt caggcgtacg ccatcaatta acaccaacac atgactggca
     2641 tttgtaccgc gaataaaaat agatgagagc tgacctgaac cgccgttttg ggtgatatcg
     2701 acgcccggaa gacggcgcag cacatcattg accgaggtcg actgccagcg gtcgatatcc
     2761 tgacgggtca caacggtggt tggtgcaagc acagtgctgc gcggctgttc aaaacggtta
     2821 gcagtaacga cgagagtatc cgggctggta tcctgtgccc aagcggaaaa ggctgtgaca
     2881 gaacacgccg tcagcagcga agctttttta atcattgtaa agcatccaca atagaagaag
     2941 gatgccgcag gtttcatcaa tattacgcga tgatgagacg ttggccggca ggtcttcggg
     3001 cttggagggg tatctaagat actaagagat gatgacttcc caccggatgg cagtgtccgc
     3061 ataacgcaat catcgcacct ttccttaccg ctgcgcgtca gctccagatt cgcactggat
     3121 tccctattaa ctcacaggac cggcaagtgg atgctacagg ttgtaacaag ttactgtcca
     3181 gacgtagctc acaaatagga attcatcaag atctggacat ctgatgagca atccctacaa
     3241 tcgccgcgta ctttaatttt tcaggataca tcatgacccc cgaacacctt ccaacagaac
     3301 agtatgaagc gcagttagcc gaaaaagtgg tacgtttgca aagtatgatg gcaccgtttt
     3361 ctgacctggt tccggaagtg tttcgctcgc cggtcagtca ttaccggatg cgtgcggagt
     3421 tccgcatctg gcacgatggc gatgacctgt atcacatcat tttcgatcaa caaaccaaaa
     3481 gccgtatccg cgtggatagc ttccccgccg ccagtgaact tatcaaccag ttgatgacgg
     3541 cgatgattgc gggtgtgcgt aataatcccg ttctgcgcca caagttgttc cagattgatt
     3601 acctcaccac gctgagtaat caggcggtgg tttccctgct ataccataag aagctggatg
     3661 atgagtggcg tcaggaagcg gaggccctgc gcgatgcact gcgcgcgcag aatctgaatg
     3721 tgcatctgat tggtcgggca acgaaaacca aaatcgagct ggatcaagat tacatcgacg
     3781 agcgtctgcc ggtcgcaggg aaagagatga tctaccgtca ggtagaaaac agctttaccc
     3841 agccgaacgc ggcgatgaat attcagatgc tggaatgggc gctggacgta accaaaggct
     3901 caaaaggcga tttactggag ctgtactgcg gcaacggtaa cttttcatta gcgctggcgc
     3961 gcaattttga tcgggtatta gccaccgaaa tcgctaagcc gtcggttgct gctgcgcaat
     4021 acaacatcgc agctaaccat attgataacg tacaaattat tcgtatggcg gcagaagaat
     4081 ttactcaggc gatgaatggt gtgcgcgagt ttaaccgcct gcaagggatc gacttaaaga
     4141 gttatcagtg cgaaaccatt tttgtcgacc caccgcgcag cggtctggac agtgaaaccg
     4201 agaaaatggt gcaggcgtat ccgtgtattt tgtacatctc ctgtaacccg gaaacgttat
     4261 gcaagaatct ggaaacatta agccagacgc acaaggtcga acgtctggct ctgtttgatc
     4321 agttccccta cacgcaccat atggagtgcg gcgtattact gaccgcgaag taaaacccat
     4381 gccggatgcg ccagcatccg gcataatacc gattactcag caacttcctg cttacggtta
     4441 cgcatcttcg cgccaatcca gaacaccata atgacggaga gtactgccgg gaagaagtta
     4501 gagccgatat ccggatattc cgcacgaact accgtgctgt acagcagtac gccgagaata
     4561 aaacaggcag ctgccagacc cggcaagcct accggcatag tgcgattaag ataacgttga
     4621 tgcaggcagt aaaccgtcag caccagggaa ataatcggga atacagaaaa tggcacaatg
     4681 gagctaaaca gcgctgcgaa agtaccatta atcgataagc cagcaactaa cgccagcagc
     4741 agcgtacctc tatcttgatt tgcttgtttc attactcgtc cttcacattt cccggaataa
     4801 ttgcggtttt ctcttgttca cggcgatacc agtaataagc ccctttcgaa atcattcgca
     4861 gttgcagtac cagtcgctct tctaattgcc gacgttgttc gacgccgacg tccaacgcct
     4921 cggcacccgc actgaagaca attgtcacca ttgcttcggc ttgcgcttca gtaaacgcac
     4981 gcggcatatg gttttcgagt tccagatagt ccgcaagttc cgcaatgaag tgctgaattt
     5041 cacgcgcaac ggcggcacga aacgcagcgg aggtgccgga gcgttcccgc aataataacc
     5101 ggaaggcgtt aggattatta ccgatgaact ccataaatgt ggagaccgag gtgcggatca
     5161 cactcccgcc tttggcgata cgctgacgcg cctggcgcat gagttggcgt agcattaaac
     5221 cgctctcatc aaccatggtc agacccagtt cgtctacgtc gcggaaatgc cgataaaaag
     5281 aggtgggagc aatgcccgct tcacgcgcca cttcacgcaa actcaggctg gcgaagctgc
     5341 gttcagcact taattggcta aatgcggctt ccaccagcga acggcgggtt ttttcttttt
     5401 gttgcgctct tatgcccatc acgatgtctg aatccttgcc aaaagtactg ctggcactat
     5461 accagagaat gaacatactg gattgctcca gtattgtgaa tgaacggtaa cgcaataaaa
     5521 catatttact ggccggttct tttacgtaca gcggaaacct gccgcttaaa cggagagtat
     5581 cgtcgataaa aatccaataa aacgtcaggg caaaagtaag aaacagacaa agcaaaggcc
     5641 gctcaggata tagccagata aatgacgggg atcaattggc ttacccgcga taaaatgtta
     5701 ccattctgtt gcttttatgt ataagaacag gtaagcccta ccatgccaca ttcctacgat
     5761 tacgatgcca tagtaatagg ttccggcccc ggcggcgaag gcgctgcaat gggcctggtt
     5821 aagcaaggtg cgcgcgtcgc agttatcgag cgttatcaaa atgttggcgg cggttgcacc
     5881 cactggggca ccatcccgtc gaaagctctc cgtcacgccg tcagccgcat tatagaattc
     5941 aatcaaaacc cactatacag cgaccattcc cgactgctcc gctcttcttt tgccgatatc
     6001 cttaaccatg ccgataacgt gattaatcaa caaacgcgca tgcgtcaggg attttacgaa
     6061 cgtaatcact gtgaaatatt gcaggggaac gctcgctttg ttgacgagca tacgttggtg
     6121 ctggattgcc cggacggcag cgtcgaaaca ctaaccgctg aaaaatttgt tattgcctgc
     6181 ggctctcgtc catatcatcc aacagatgtt gatttcaccc atccacgcat ttacgacagc
     6241 gactcaattc ttagcatgca ccacgaaccg cgccatgtac ttatctatgg tgctggagtg
     6301 atcggctgtg aatatgcgtc gatcttccgc ggtatggatg taaaagtgga tctgatcaac
     6361 acccgcgatc gcctgctggc atttctcgat caagagatgt cagattctct ctcctatcac
     6421 ttctggaaca gtggcgtagt gattcgtcac aacgaagagt acgagaagat cgaaggctgt
     6481 gacgatggtg tgatcatgca tttgaagtcg ggtaaaaaac tgaaagctga ctgcctgctc
     6541 tatgccaacg gtcgcaccgg taataccgat tcgctggcgt tacagaacat tgggctggaa
     6601 actgacagcc gcggacagct gaaggtcaac agcatgtatc agaccgcaca gccgcacgtt
     6661 tacgcggtgg gcgacgtgat tggttatccg agcctggcat cagcggccta tgaccagggg
     6721 cgcattgccg cgcaggcgct ggtaaaaggc gaagccaccg cacatctgat tgaagatatc
     6781 cctaccggca tttacaccat cccggaaatc agctctgtgg gcaaaaccga acagcagctg
     6841 accgcaatga aagtgccata tgaagtgggc cgcgcccagt ttaaacatct ggcacgcgca
     6901 caaatcgtcg gcatgaacgt gggcacgctg aaaattttgt tccatcggga aacaaaagag
     6961 attctgggta ttcactgctt tggcgagcgc gctgccgaaa ttattcatat cggtcaggcg
     7021 attatggaac agaaaggtgg cggcaacact attgagtact tcgtcaacac cacctttaac
     7081 tacccgacga tggcagaagc ctatcgggta gctgcgctaa atggcttaaa ccgcctgttt
     7141 taacacttta tcgaaatggc catccattct tgcgcggatg gcctctgcca gctgctcata
     7201 gcggctgcgc agcggtgagc caggacgata aaccaggcca atcgtgcggc gaggttccgg
     7261 cttaatacac ggcaaataaa caaccccatc gcgtttgcgc tccggcggca cagccagcgc
     7321 aggcagtaaa gtgatcccgc tacctgccgc caccatgtta cgcagtgtct ccaggctggt
     7381 cgcgcggaag tgtgtatctt catccgcccc ggcttcaaag cagaaaccca ttgcctgatc
     7441 gcgcaaacag tgaccatctt ccagcatcag cagtttttcc cctgccagat cggccatcgg
     7501 tacgcattcg cggttcgccc acgggtgatc ttcatagata gccagcaaca ttggctcatc
     7561 aaacaacggc acttcaatga atgcttcgct ctctttcacc agcgcgagga tcacgcaatc
     7621 gagtttgccg ctgtccagtt gcgccagtaa ctggtgggtc tgtgcttcat gcagatacat
     7681 ttccagcttt ggaaatgtct ggtgcagcat cgggataata tgcggcagca ggtacggtcc
     7741 aaccgtagga atcaaaccaa tatgcagcgg tccggacatc gtctcgccct gctggcttgc
     7801 catctcttta aggactttca cctcacgcag cacggtacgc gcctgatcca ccagcagcat
     7861 tcccgcctgg gtgaacaaca ctttacggct ggtccgctcc agcaacatca cgcccagctc
     7921 atcttccagc ttacgaattt ggccgctaag cgtcggctgg ctaacgtggc aggaatctgc
     7981 cgcacgccga aaatggcggt gttcagccaa tgccaccagg tactcaagat cacgaatatt
     8041 cattatccat cctccatcgc cacgatagtt catggcgata ggtagaatag caatgaacga
     8101 ttatccctat caagcattct gactgataat tgctcacaga aacggagcgg cacctctttt
     8161 aacccttgaa gtcactgccc gtttcgagag tttctcaact cgaataacta aagccaacgt
     8221 gaacttttgc ggatctccag gatccgcttt tttttgccat aaaaaagccc ggcgataagc
     8281 caggctcaaa tttgtacata taaatgttct taccccaacc gcgcctgcgc aaaagcaatc
     8341 gcttgggcca cctgctgcgg tgagacgccg ccttttgccg cacgcttgtc gaggcacgat
     8401 tgtagcgaca gaatcggata gacatcttcg ccaatcacgt ggctgaattt ctgcaactcg
     8461 tcgagcggca gatcttccag cggtttgccc tgacgaatgg cttccaccac cgcttcaccc
     8521 acgatatggt gcgcctcgcg gaaaggtacg cctttcgcca ccaggtaatc cgccagttcg
     8581 gtggcgttgg cgtaaccctg ctgagccgct tcctggcaac gtggacgttt cacctgaatg
     8641 ccgtccagca ccagcgccgc catatgcagg cagtccagcc aggtatcgaa cgcgtcgaac
     8701 agaccttctt tgtcttcctg catatctttg ttgtaagcca gcggcaaacc tttcagcgtc
     8761 atcatcatgc cggttaacgc accctgcacc cgaccgcatt taccgcgaat cagctccagc
     8821 gcatccgggt ttttcttctg cggcattaat gatgaaccgg aagtcacgcg gtcagaaagc
     8881 tccacaaacc ccgcttcgcc ggtgttaaag aaaatcagat cttcagcaag acgcgacaga
     8941 tgcaccatgc cgatagcggc agccgacagc agttccaaca catggtcacg gtcagaaacg
     9001 ctgtccagac tgttacgggt cgccgaagca aagcccagcc agcctgctaa ctgttcacgg
     9061 tcgatttcat aggccgttcc cgccagcgcg ccacagccta atgggctgac atccagacgc
     9121 ttaagcgcat cctgcaaacg gctttcatca cgcgccagca tctcaacata ggccaggcac
     9181 cagtgcgcga acgtcaccgg ctgggcgcgt tgcaggtgag tgtaacctgg cattaccgcg
     9241 tcctgattgt tttgtgcggt ttccaccagc gccgattgca gctgccggtt agccgtcagt
     9301 aactcgctaa cggtatcttt gcaccacagt ttcaggtcag tcgctacctg atcattacgg
     9361 ctacgcccgg tatgcagctt tttgcctaac tggcccactt tgtcgatcag tttgccttcc
     9421 acccagctat ggatatcttc ggcgtcgctt tcaaggattt gttgtggcct ggcgcgaaca
     9481 tcttccagca acacgttcag cgcctcttcc agttgcgcct gctcttctgc ggttaacacg
     9541 ccgaccgtta ccagggcttt ggaccaggcc acagagccaa caatatcctg ctccgccaga
     9601 cggtaatcaa agcgcagtga gtcgttgaat tgtttgaacc gttgatctgc tgcctgggta
     9661 aaacgcccgc cccaaagtgc cataactctg tttccttatt ttgaaattca atgccggaaa
     9721 gctgcgcctc cggctaacaa aacttaagct aaaatccgcg tacccatcgg cataccgtta
     9781 aacagtgccg gaagctgctc cgcatgacgc caggaggcga tatctaccgg acggcccagc
     9841 gtgcgggccg catccagcgc cgcgttcact ttcactatca tgccgtcagt aataatgccc
     9901 tgctcaatca gttgttctgc tttcgcggcg gtcatttcgg caatgcgttg ccctttgccg
     9961 tcgagaatgc cgctgacgtc ggagagcaaa atcagatccg cgcccagcgt tgccgccagc
    10021 gccgttgccg cctggtcggc attgacgttc atcagttgcc cttcgtctgt tacgccaatg
    10081 gagctgacca ccggcagata accgttctcc agcaaggagt tgataagctt aggcgaacct
    10141 ggctgcgcca gtccaacatg acctaactct tcatcaagct gggtcacttt gacgctgtcg
    10201 ccatcaccga gaaacaaacc tacagccgca atctgatgtt tcttcgccca tgccaacagg
    10261 gttttatttg ccgttcccgc cagtgctccg gtgataatgt ctatctgatc agcaggcgtc
    10321 acccgcaggc cgtttttctt tttcaccggc agattcagcc ctttcatcag ctcatccacc
    10381 acgcaaccgc cgccgtgcac aatcaccagc ggacgctgat gtgactcacg ataattcacc
    10441 agtgcgctaa acagacgttc cagcgcctct tcactatcca gcagtacgcc gcccagtttg
    10501 ataattaatg gattcatcat tgcaccctta aataagagac tgcgtttccg catagccgaa
    10561 acgaatattg gcgcactgta ccgcttgtgc cgccgcgcct ttcagtaagt tgtcttcggt
    10621 cgccacaata atcagatgct cgccctgaac ggcaaacccg atatcgcaaa atggcagccc
    10681 aacgacattt ttcagcgccg gaacgccttt gtcatacagc cgcaccagcg gtttatgggc
    10741 atacgcctgc tgtaacactt gcgcgacttg cgcctgggtc acacccgatt tcaggcggca
    10801 ggtaatggtt tcgagaatgc cgcgcgggaa attgcccaga tgtggggtga agataacgtc
    10861 agcaccgagg tgtgtggcga tctctggttg atggcgatga gtaaagacgc catacggttg
    10921 caggctaact tcacaaaaac tatttgaaat cgctgcctta cgtcctgcac cgctcacgcc
    10981 gctggtggcg ttaatcgccg gccactgatt gaggtcaaga agatcggcat caatcaacgg
    11041 tttcagcgcc agctgtgccg ccgtcggata acagcctggc accgcaatca aattcgcttc
    11101 ttttaattta ttaccgcacc actccgccag accgtaggct gcctgttcca gcagttccgg
    11161 gtattgatgg gtaaagccgt aatatttttc atagaaggtg gcgtcgttaa cacgaaacgc
    11221 gccggaaagg tcgaacacca cgcagcccgc ttcaagaaac tgcggcgcta aatcgtggct
    11281 aacttcatgg gcggtggcga gaaacactac gtccacccct gggctaaact cgctgatatc
    11341 cgacatcggc tgcaacggca gctcaacgat gccttttagc tgcggatgca aatcggagat
    11401 taactttccc gcatcattgc tttgcgctga aacagtcaaa gcggttatgt tcatatgcgg
    11461 atggcgattt acataggtca ctagctctgc gccagcgtag ccgctggcac ccacaatcag
    11521 cgtattcaac atcggggcta ttcaccttct tatgtctggt tgccaggtta aacgtaaaac
    11581 attcacctta cggctggtgg gttttattac gctcaacgtt agtgtatttt tattcataaa
    11641 tactgcatga atattgatac tatcatgacc agaggtgtgt caacaatgaa aaacaaatta
    11701 ccgccattta tcgagattta ccgcgctctg attgccacac cttcaataag cgctacggaa
    11761 gaggcactcg atcaaagcaa tgcagattta atcactctgc tggcggactg gtttaaagat
    11821 ttgggcttca atgtggaagt gcagcctgtt ccaggaactc gcaacaaatt caatatgctg
    11881 gcaagtatcg gacagggggc tggcggcttg ttgctggcgg ggcataccga tacggtgcca
    11941 tttgatgacg gtcgctggac gcgcgatccg tttacactga cggagcatga cggcaagctt
    12001 tacggcttag gcaccgccga catgaaaggc ttttttgcgt ttatccttga tgcgctacgc
    12061 gatatcgacg tcacgaaact gaaaaaaccg ctctacattc tggcgactgc tgatgaagaa
    12121 accagtatgg ccggagcgcg ttattttgcc gaaactaccg ccctgcgccc ggattgcgcc
    12181 atcattggcg aaccgacgtc actacaaccg gtacgcgcac ataaaggtca tatctctaac
    12241 gccatccgta ttcagggtca gtcggggcac tccagcgatc cagcacgcgg agttaacgct
    12301 atcgaactaa tgcacgacgc catcgggcat attttgcaat tgcgcgataa cctgaaagaa
    12361 cgttatcact acgaagcatt taccgtgcca taccctacgc tcaacctcgg gcatattcac
    12421 ggtggcgacg cttctaaccg tatttgcgct tgctgtgagt tgcatatgga tattcgtccg
    12481 ctgcctggca tgacactcaa tgaacttaat ggtttgctca acgatgcatt ggctccggtg
    12541 atcgaacgct ggccgggtcg tctgacggtc gacgagctgc atccgccgat ccctggctat
    12601 gaatgcccac cgaatcatca actggttgaa gtggttgaga aattgctcgg agcaaaaacc
    12661 gaagtggtga attactgtac cgaagcgccg tttattcaaa cgttatgccc gacgctggtg
    12721 ctcggtccgg gttccatcaa tcaagctcat cagcctgatg aatatctgga gacgcggttt
    12781 attaaaccca cccgtgagtt gattatccag gtaattcacc atttttgctg gcattaaaac
    12841 gtaggccgga taaggcgctc gcgccgcatc cggcaccgtt gccaaactcc ggtgccgcaa
    12901 taatgtcgga tgcgatgctt gcgcatctta tccgacctac acctttggtg ttatgtatgg
    12961 cgatttttta acatttccat aagttacgct tatttaaagc gtcgtgaatt taatggcgta
    13021 aattcctgct atttattcgt ttgctgaagc gatttcgcag catttgacgt caccgctttt
    13081 acgtggcttt ataaaagacg acgaaaagca aagcccgagc atattcgcgc caatgcgacg
    13141 tgaaggatac agggctatca aacgataaga tggggtgtct ggggtaatat gaacgaacaa
    13201 tattccgcat tgcgtagtaa tgtcagtatg ctcggcaaag tgctgggaga aaccatcaag
    13261 gatgcgttgg gagaacacat tcttgaacgc gtagaaacta tccgtaagtt gtccaaatct
    13321 tcacgcgctg gcaatgatgc taaccgccag gagttactca ccaccttaca aaatttgtcg
    13381 aacgacgagc tgctgcccgt tgcgcgtgcg tttagtcagt tcctgaacct ggccaacacc
    13441 gccgagcaat accacagcat ttcgccgaaa ggcgaagctg ccagcaaccc ggaagtgatc
    13501 gcccgcaccc tgcgtaaact gaaaaaccag ccggaactga gcgaagacac catcaaaaaa
    13561 gcagtggaat cgctgtcgct ggaactggtc ctcacggctc accctaccga aattacccgt
    13621 cgtacactga tccacaaaat ggtggaagtg aacgcctgct taaaacagct cgataacaaa
    13681 gatatcgctg actacgaaca caaccagctg atgcgtcgcc tgcgccagtt aatcgcccag
    13741 tcatggcata ccgatgaaat ccgtaagctg cgtccaagcc cggtagatga agccaaatgg
    13801 ggctttgccg tagtggaaaa cagcctgtgg caaggcgtac caaattacct gcgcgaactg
    13861 aacgaacaac tggaagagaa cctcggctac aaactgcccg tcgaatttgt tccggtccgt
    13921 tttacttcgt ggatgggcgg cgaccgcgac ggcaacccga acgtcactgc cgatatcacc
    13981 cgccacgtcc tgctactcag ccgctggaaa gccaccgatt tgttcctgaa agatattcag
    14041 gtgctggttt ctgaactgtc gatggttgaa gcgccccctg aactgctggc gctggttggc
    14101 gaagaaggtg ccgcagaacc gtatcgctat ctgatgaaaa acctgcgttc tcgcctgatg
    14161 gcgacacagg catggctgga agcgcgcctg aaaggcgaag aactgccaaa accagaaggc
    14221 ctgctgacac aaaacgaaga actgtgggaa ccgctctacg cttgctacca gtcacttcag
    14281 gcgtgtggca tgggtattat cgccaacggc gatctgctcg acaccctgcg ccgcgtgaaa
    14341 tgtttcggcg taccgctggt ccgtattgat atccgtcagg agagcacccg ccataccgaa
    14401 gcgctgggcg agctgacccg ctacctcggt atcggcgact acaaaagctg gtcagaggcc
    14461 gacaaacagg cgttcctgat ccgcgaactg aactccaaac gtccgcttct gccgcgcaac
    14521 tggcaaccaa gcgccgaaac gcgcgaagtg ctcgatacct gccaggtgat tgccgaagcg
    14581 ccgcaaggct ccattgccgc ctacgtgatc tcgatggcga aaacgccgtc cgacgtactg
    14641 gctgtccacc tgctgctgaa agaagcgggt atcgggtttg cgatgccggt tgctccgctg
    14701 tttgaaaccc tcgatgacct gaacaacgcc aacgatgtca tgacccagct gctcaatatc
    14761 gactggtatc gcggcctgat tcagggcaaa cagatggtga tgattggcta ttccgactca
    14821 gcaaaagatg cgggcgtgat ggcagcgtcc tgggcgcaat atcaggcaca ggatgcatta
    14881 atcaaaacct gcgaaaaagc gggtattgag ctgacgttgt tccacggtcg cggcggttcc
    14941 attggtcgcg gcggtgcacc tgcccatgcg gcactgctgt cacaaccgcc aggaagcctg
    15001 aaaggcggcc tgcgcgtaac cgaacagggc gagatgatcc gctttaaata tggtctgcca
    15061 gaaatcaccg tcagcagcct gtcgctttat accggggcga ttctggaagc caacctgctg
    15121 ccaccgccgg agccgaaaga gagctggcgt cgcattatgg atgaactgtc agtcatctcc
    15181 tgcgatgtct accgcggcta cgtacgtgaa aacaaagatt ttgtgcctta cttgcgctcc
    15241 gctacgccgg aacaagaact gggcaaactg ccgttgggtt cacgtccggc gaaacgtcgc
    15301 ccaaccggcg gcgtcgagtc actacgcgcc attccgtgga tcttcgcctg gacgcaaaac
    15361 cgtctgatgc tccccgcctg gctgggtgca ggtacggcgc tgcaaaaagt ggtcgaagac
    15421 ggcaaacaga gcgagctgga ggctatgtgc cgcgattggc cattcttctc gacgcgtctc
    15481 ggcatgctgg agatggtctt cgccaaagca gacctgtggc tggcggaata ctatgaccaa
    15541 cgcctggtag acaaagcact gtggccgtta ggtaaagagt tacgcaatct gcaagaagaa
    15601 gacatcaaag tggtgctggc gattgccaac gattcccatc tgatggccga tctgccgtgg
    15661 attgcagagt ctattcagct acggaatatt tacaccgacc cgctgaacgt attgcaggcc
    15721 gagttgctgc accgctcccg ccaggcagaa aaagaaggcc aggaaccgga tcctcgcgtc
    15781 gaacaagcgt taatggtcac tattgccggg attgcggcag gtatgcgtaa taccggctaa
    15841 tcttcctctt ctgcaaaccc tcgtgctttt gcgcgagggt tttctgaaat acttctgttc
    15901 taacaccctc gttttcaata tatttctgtc tgcattttat tcaaattctg aatatacctt
    15961 cagatatcct taaggaattg tcgttacatt cggcgatatt tttttcaaga caggttctta
    16021 ctatgcattc cacagaagtc caggctaaac ccctttttag ctggaaagcc ctgggttggg
    16081 cactgctcta cttttggttt ttctctactc tgctacaggc cattatttac atcagtggtt
    16141 atagtggcac caacggcatt cgcgactcgc tgttattcag ctcgctgtgg ttgatcccgg
    16201 tattcctctt tccgaagcgg attaaaatta ttgccgcagt aatcggcgtg gtgctatggg
    16261 cggcctctct ggcggcgcta tgctactacg tcatctacgg gcaggagttc tcgcagagcg
    16321 ttctgtttgt gatgttcgaa accaacacca acgaagccag cgagtattta agccagtatt
    16381 tcagcctgaa aattgtgctt atcgcgctgg cctatacggc ggtggcagtt ctgctgtgga
    16441 cacgcctgca cccggtctat attccaaagc cgtggcgtta tgttgtctct tttgccctgc
    16501 tttatggctt gattctgcat ccgatcgcca tgaatacgtt tatcaaaaac aagccgtttg
    16561 agaaaacgtt ggataacctg gcctcgcgta tggagcctgc cgcaccgtgg caattcctga
    16621 ccggctatta tcagtatcgt cagcaactaa actcgctaac aaagttactg aatgaaaata
    16681 atgccttgcc gccactggct aatttcaaag atgaatcggg taacgaaccg cgcactttag
    16741 tgctggtgat tggcgagtcg acccagcgcg gacgcatgag tctgtacggt tatccgcgtg
    16801 aaaccacgcc ggagctggat gcgctgcata aaaccgatcc gaatctgacc gtgtttaata
    16861 acgtagttac gtctcgtccg tacaccattg aaatcctgca acaggcgctg acctttgcca
    16921 atgaaaagaa cccggatctg tatctgacgc agccgtcgct gatgaacatg atgaaacagg
    16981 cgggttataa aaccttctgg atcaccaacc agcagacgat gaccgcccgc aataccatgc
    17041 tgacggtatt ttcgcgccag accgacaagc agtactacat gaaccagcaa cgtacgcaga
    17101 gtgcgcgtga atacgacacc aacgtgctga agccgttcca ggatgtgctg aatgaccctg
    17161 cgccgaagaa actgatcatc gttcatctgc tgggtacgca tatcaaatac aaataccgct
    17221 acccggaaaa tcagggcaag tttgatggca ataccgatca tgttccgcca ggattaagcg
    17281 cagaagagct ggaatcatat aacgattatg acaacgctaa cttgtataac gatcatgtgg
    17341 ttgccagcct gattaaagac tttaaagcgg cagacccgaa cggattcctg gtttacttct
    17401 ctgaccacgg tgaagaggtt tacgacacgc cgccgcacaa aactcagggg cgtaacgaag
    17461 acaacccgac gcgccacatg tacaccattc cgttcctgct gtggacgtcg gaaaaatggc
    17521 aagcgactca tccccgtgat ttctcacagg atgtcgatcg taaatacagc ctggcggaac
    17581 tgatccacac ctggtcagat ttggcgggct tatcttacga cggttacgat ccaacccgtt
    17641 cagtggtgaa tccgcagttc aaagaaacta cccgctggat tggtaacccg tacaagaaaa
    17701 acgcgctgat cgattacgac actctgccgt atggcgacca ggtaggtaat cagtaattat
    17761 cagccaggcg ctatttgccg gatgcgacgc ttgacgcgtc ttatccggcc tacagatcgc
    17821 ggcggtaggc tggataaggc gtttacgcca catccggcaa tacaactgcc gcaatgttta
    17881 catcgaactc ccctcaggcc acacttttca ggtggccttt tttaataatg ccagttactg
    17941 ttttcccgtc tacataccta agggaataac atgtatcacg acatcagcta tctgctctcc
    18001 cgcctgatca acggcccgct gtccctgcgc cagatttact ttgccagcag taacggcccg
    18061 gtgcccgatc ttgcgtatca ggtcgatttt ccacggctgg aaattgtgct ggaaggtgag
    18121 tttgttgata ccggcgctgg agcaacgtta gttcccggcg atgtgctgta cgttgccgct
    18181 ggtggctgga atttcccaca atggaaaaca cccgctacca cctttagcgt gctgtttggc
    18241 aaacagcaac tcggcttcag cgtcgtgcaa tgggatggca aacaatatca aaatctggcg
    18301 aagcaacacg tcgcccgacg cggcccacgt ataggttctt ttctgctaca aacgctcaat
    18361 gaaatgcaaa tgcagccgca ggagcagcaa acggcaaggc ttatcgtagc cagcctgctt
    18421 agccactgcc gcgatttgct cggcagccaa atccagaccg cctcacgcag ccaggcacta
    18481 tttgaagcta ttcgcgatta tatcgacgaa cgctatgcct ccgcgcttac ccgcgaatct
    18541 gtcgcacagg cattctatat ttcaccaaac tatctctcgc acctgtttca aaaaacgggg
    18601 gccattggtt ttaacgaata cctgaatcac acgcgactgg agcacgctaa gacgttattg
    18661 aaaggctatg acctgaaggt aaaagaagtg gcgcacgcct gcggctttgt cgacagcaac
    18721 tacttctgcc gtctgtttcg taaaaacacc gaacgctcgc cgtcggaata tcgccgacag
    18781 taccacagcc aactgacaga aaaaccgact actccagaat aagatgggtt tgctgcggcg
    18841 cagaaagcac tttgcgcacc gcgctcatta cccgctgcgg ctcacgcagg aatgcgtaga
    18901 tgctgcattg cacatagcgg caatgttcaa atcgctcggc accggcaagc tcgatatccg
    18961 taatcaacag agcaacatcc gcccgacgga tatcctcgtc cgctaaacga ttctccgttc
    19021 ccagcgcgcc ctgagtttca atgctgacgc cccacttctc taactggcac aacttttcca
    19081 gccgttccgc cgccatataa gtatgcgcca cgccgcttac gcaggcggtt actgccacca
    19141 ggtatgccat tttaacctcc cacggtaacc tgaaatccgg cccgttctgc catttcgcgc
    19201 atcgttgcca catcggctga tgacggcgca gcaacatcct tcatcgacca tgttttcccc
    19261 agcaggcggt atttcggctc gccgtactga tgaaacggca acagatggat ctgcctgata
    19321 ttcagcggga tcagcacgtc cagcgcctgc tgcatattct cccggctgag tgtgaaaccg
    19381 gggatcagcg gcaaacgcgg gatcacgttg acaccctcac tcaccagcaa acgcaggttc
    19441 tccagtacgc gtggcaggtt catcttcacc acatcccgcg cctgagtcgc gtccataatt
    19501 tttaaatcga acaacacttc atcgcacaat ttcgccagcg gtaacagctt gctggctggt
    19561 gcgtctccgg cagtttcaat ggcacatgag acaccccaca gccgcagtcg ctgtaaaaaa
    19621 cgggtagcaa actccgcctg cattaacact tcgccgccag aaagcgtgac gccgccgccg
    19681 gacgtgcgaa aaaaaatgtc atctttcatc acttcccgtt ccagagcgtc aaggctgata
    19741 tcgcgaccaa tccgttcaaa cgccccggag gggcattcat ccgcatcacg caaacatttc
    19801 gcacagtgca gacatttcgc ctctctgcgt accgtctgga ttttgccgga gatcgactcc
    19861 ggattagcac accacgggca aagatgcgga cagcctttaa aaaagaccac cgtacgaatg
    19921 ccctcgccgt cattcagtga ataacgctga atgttgaaaa tgcgcgccac atcattgagg
    19981 cgcgtttcca caacgttaca gctgatgcgc tgtccggcgg atgatgtcat cctggatctc
    20041 cttcgacagt tcgacaaaga aggcgctgta tccggcaacg cgcaccacca gcccggcata
    20101 atcttgtggg cgctgttgcg cttcccacaa cgtgtcggcg ttcaccacgt taaactgaat
    20161 atgctgcaac ttaagctggg taaacgcccg taagaagtcg gccagtttgc gtaatcccgc
    20221 ttcaccttcc agggtcgccg gagtgaattt cacgttcagc aacgtaccgt tagacagcag
    20281 cgtattatcg agcttactga ctgacttcag taccgctgtt ggcccttgtt cgtcctggcc
    20341 cagcataggt gacaagccgc cgtctgccag ctgttctccg gcaaaacgac cgtctggcgt
    20401 cgcgccaacc accgatccca acgggacgtg agcagaaacg gtatacgatc ccggcgtgaa
    20461 gtaaccaccg cgcgggttct ggtatttttc cacttctttg cagtagtggc gcaacagttc
    20521 ggcgctaatg ttatccacct cgtcgatatc gttaccatat ttctcaaagc ggttaattaa
    20581 gcgagcgcgg actttttcgc cttctggcgt tgcaaagttg gcttttaata ccgacagcaa
    20641 ttcgtcaaaa cttaaacgct gttgctcaaa aaccatccct ttcaacgcat ggagagaatc
    20701 gctcaggttg gcgataccga tcccctgtac gccggagaag ttataacgcg cgccgccatc
    20761 ggtaatatcg cgaccttttt ccagacaatc gctgataaaa gacgagagca gcggtacagg
    20821 tgcccagtcg cgatggccga tatcacaaat attgctgccc tcaaccatca gggtgatgta
    20881 gtggctgatc ttggcgcgga tctgctccag taaaccttca taagtcagcg cagcattgcc
    20941 ttcattttca tgcaggcaga tttccatcac tttcagcaga ttaaacatcg cgatgtcatg
    21001 caagccgtag gttctgccgg gaatagataa ttccacacag cccactacgg aatagtcgcg
    21061 cgcatcttcc agcgaaacgc cacggttgag gaacgctggt accaccactt catcgttaaa
    21121 gatttgcgga ataccggtgc cgaggcgaat ggtttcggcg gttttcatca ggaatggcgt
    21181 gtcgatcaag gcgttagtgc gcacgccgag gttcggttgc ggtaattgca cgctttgata
    21241 ggcgtcaagg caaaggaacg aaagcacgtt caccgcgcta cgtccgttct cggttaaccc
    21301 gccgagcagt gcggtatagc cggtcgggaa acctgcgaaa taacgcgcgc tgctggtgga
    21361 gcgcaacagc acgatgtcgt tgcatttcac ccataaagat tcgagcagtt ctttcaggaa
    21421 cgccggatct tcgcccgggg ttaaagatgc ctgatagaac ggcaacatat actggtcgaa
    21481 gcgccctaaa gacagcgaac tggcgttgga ttcgtattgc agaatgatgt tcatgtacca
    21541 gaataactgg cacgcctgcc agaacgtctg cggcttatgt tcggcgttat ggcgagagat
    21601 ctccgcaata gtcagcagtt cttcgcgacg ctgggcatct gtgcagtttg ccgccatcgt
    21661 ttccgccagt acagcgtaac gcaaaatgtg tttctgcgag gcttccagca gtaacagcgc
    21721 tgcctgataa aagtgattct ccggctgttg ctgacaatgt tgctgcatct gtgccaccaa
    21781 ctcccccagc ccgtgattca acaggcgtgg gtaatcaata ataatgtgcc cctggccttt
    21841 atccgtctgg ttaatgctga aaatctgcgt gctggtcgcg gcttttactt catccgtcat
    21901 ctgcccgttg atgaaatctt tcatcgaacg tttttcccag tacgggaaca actcttcacg
    21961 atagatacgt ttatcttctt cgctgatagc aaagcggtcc tgcggacgcg tcgggaattg
    22021 atccagctct ttcagcagcc agtaagggtc catttccggc gacataatcc cggcgcgcgg
    22081 ttttacggtg cggttaccgg caatcagttc ttcatcacga atcgaaattt caacatgttc
    22141 aaggatatac gctgttgctt tcgcccggcg caatatcacc ggttcgcctt cggtttgccg
    22201 atggctggcg gtataaagca gcgcccgctc cagcgagatt tcacgggtat tggcaaacag
    22261 tgcagttttg aggcgagaga tacgattcgt catgaggaac tcctgtggga aagcgaaccc
    22321 gcccttgcga gcgggagaag aacttaagcg gtttgcgcca ggtgagcttc aattttgctc
    22381 ataatggcgt cagcacgttt tactgcatcg ctgatgttga cgcgcacgat ggtcttgcca
    22441 gcaaaacgct cttcaaactt gatgccgata tctttggtca gaatcaccat atcagcgctc
    22501 gccacatctt ccgcagtcag ttcattttcc agaccaattg acccctgggt ttctactttc
    22561 acttcccagc ctttcgcttt cgcggcactt tccagcgcct ctgctgccat ataggtatgg
    22621 gcaacacctg aaggacatgc ggttactgca ataattttcg tcatggctcg ttcctcaatt
    22681 aattaatttc aaaatccaaa tccaggtcgt cttctttttc atcagtcgaa gacccatttt
    22741 tacgcgccag acttttcagc acgttcacac aaacagccgt caccactgct ccgacagcca
    22801 ctgctgcgat ataacccagc ttgccttcaa ccaccggcag cacaatcaga ccaccccagc
    22861 ctgcatagca ctgcgcaccg accagcgccg cagttactgc accacaaact gaaccgacca
    22921 tgatggaagg aatgacacgc agcggatcgg cagcggcgaa aggaatcgcc ccttcagtaa
    22981 cgccaacaca gcccatcacc agtgccgctt tgccggtttc gcgctcttct gcggagaaat
    23041 ttttacgacc aatcagcgtc gccagcccca tccccagcgg cgggatacaa atacccacgg
    23101 cagcgatgac caccacggta taaacaccct gagcaacgca aatcagcatg aaggcatagg
    23161 ctactttgtt aaccggaccg cccatatcga acgccagcat cagacccatg atcaccgcca
    23221 gcataacaat gctgccctgc tgcatcccct gaagccactg agtcaggctg ttggtcaacg
    23281 cccctacagg ctcgcccaag ccccacatca tgatgcctgc ggtaatcagt gtgccgacga
    23341 taggaataat gaagataggc atcaccgagc gcagaacttt atgcaccgga attttcttca
    23401 ggtaatgcac cacgatgccg ccgataatcc cggcgatcag tgcaccaaag aagcccgcac
    23461 caaagctgtt accaacccag gcaccgatag cgcacggagc cagcgcagaa cgctctgcaa
    23521 tggagtaacc aatgtaagcg gcgaggaaag gcaccatcag cgtcaaacct gcaacgccga
    23581 tatcaaacag ttttttcaaa ttcggatcgg ctacggcatc cggcactgcg cctttgccat
    23641 acaacatgac ggaaaccgcc agcaaaatac cgcccgatac cacgaaggga atcatgtgtg
    23701 aaacgcccgt cattaaatgc tgacgggtat ttttcaggat ctgcaccaac tcattcataa
    23761 gctgctcccg ggcttgcgaa agcccatgtc catatttttg atggaggaaa caataggcaa
    23821 tcgcaggcgg agcaaacagt ggataaaaag gccatttact ggacttttgt aatatcagta
    23881 caaaaatgcg atccgcctca taacttgcga taaaccggaa gcatgccgca aaacggcgtc
    23941 ttgtgagacg cgtcgcaaat tgacggttac attacatttg tccagctttt ggcagatttg
    24001 tcacatggca ggccgttgcc gtgcgctctt atcctgtaaa cagaaaaccg taccgggaga
    24061 gaggcaatgg ccctgattgt ggaatttatt tgtgagctac ctaacggcgt acatgcgcgt
    24121 ccggcaagcc acgttgaaac gctgtgtaat actttttcat cacaaattga gtggcataac
    24181 ctgcgcactg accgcaaggg caacgccaaa agcgcccttg cgctgattgg caccgatacg
    24241 ctggcgggcg ataactgcca gttactgatt tccggggccg acgaacagga agcgcaccag
    24301 cgtttaagcc aatggctgcg cgatgaattc ccccactgcg atgcgccgct ggcggaagtt
    24361 aaatctgacg aactggaacc actgccggtt tcactgacca atctgaatcc gcaaattatc
    24421 cgcgcccgca ccgtgtgcag cggtagtgca ggcggcattc tgacgccgat ctcttcttta
    24481 gatctcaatg cgctgagtaa tcttcccgca gccaaagacg ttgacgccga gcaatccgca
    24541 ctggaaaatg gcctgacgct ggtactgaaa aacattgagt ttcgtctgct ggatagcgac
    24601 ggtgctacca gctcgatact ggaagctcac cgatccctgg ctggcgatac atctttacgt
    24661 cagcacttac tgacaggcgt cagcgaggga ttaagctgtg ccgaagcgat tgtcgccagt
    24721 gcgaatcact tttgcgaaga gttcgcccgt tccagcagca gctacctgca agaacgtgcc
    24781 ctggacgtac gcgacgtctg cttccagtta ctccagcaaa tctacggtga gcaacgcttc
    24841 ccggcaccgg gcaaactgac gcagcccgcc atttgtatgg ctgatgaact gactcccagc
    24901 cagttcctcg aactggataa aaatcacctc aaaggtctgt tactgaaaag tggcggcacc
    24961 acctcacata ccgtaattct tgcccgttcg ttcaacattc cgacactggt tggtgtagat
    25021 attgatgccc ttactccgtg gcagcatcaa acgatttata tcgacggcaa cgccggggcg
    25081 attgtggttg agccagggga agccgtggcc cgttattatc agcaagaagc ccgcgtacag
    25141 gacgcccttc gtgagcaaca gcgtgtctgg ctgacccaac aagcccgtac cgctgacggt
    25201 atccgcattg aaattgccgc taacatcgct cactccgtgg aagcgcaggc cgcattcggc
    25261 aatggtgcgg aaggcgttgg tttgttccgc actgaaatgc tctatatgga tcgcaccagt
    25321 gcaccgggcg aaagcgagct gtacaacatt ttttgtcagg cgctggagtc tgccaacgga
    25381 cgcagcatta ttgtgcgcac tatggacatt ggcggcgaca aacccgttga ttatctgaac
    25441 attccggcag aagctaaccc gttcctcggt tatcgcgctg tgcgtattta tgaagaatac
    25501 gcctcgctgt tcaccacaca actacggtcg atcctgcgcg cctccgctca cggcagcctg
    25561 aaaatcatga tcccgatgat ctcctcaatg gaagagatct tatgggtgaa agaaaaactg
    25621 gcggaagcca aacagcaact gcgtaacgaa cacattccgt ttgatgagaa gatccagctt
    25681 ggcattatgc tggaagtacc gtcggtgatg ttcatcatcg atcaatgctg cgaagagatt
    25741 gatttcttta gtattggtag taatgacctg acgcaatatc tgctggcagt ggatcgcgat
    25801 aacgctaagg ttactcgtca ctacaacagc ctgaatccgg cattcttgcg ggcgctcgat
    25861 tacgccgtgc aggcggtgca tcgccagggc aaatggattg gtctgtgcgg tgagctggga
    25921 gcgaaaggtt ccgtgctgcc gttgctggtc ggcttagggc tggatgaact cagcatgagc
    25981 gcaccatcaa ttccggcggc gaaagcgcgg atgacgcaac ttgatagccg tgagtgccgc
    26041 aagttgctca accaggcaat ggcctgccgt acttcgctgg aagtggaaca cctgctggcg
    26101 caattccgca tgacccaaca ggacgcaccg ctggtcaccg ccgagtgcat cacgctggaa
    26161 agtgactggc gcagcaaaga agaagtgctc aaaggcatga ccgataacct gctgctggcg
    26221 ggccgctgcc gctatccgcg taaactggaa gccgacttat gggcgcgcga ggccgttttc
    26281 tctaccggtc tgggctttag ttttgccatt ccacacagca aatcagaaca cattgagcaa
    26341 tccaccatca gcgtggcgcg tctgcaagcg ccggtgcgct ggggcgatga tgaagcgcaa
    26401 ttcatcatta tgttaaccct gaacaaacac gctgcgggcg atcagcacat gcgcattttc
    26461 tctcgcctcg ctcgccgcat catgcacgaa gaattccgta acgcgctggt taacgccgcc
    26521 tctgccgacg ctatcgccag cctgctgcaa catgaactgg aactgtaaaa ggaaacatca
    26581 tggaactgta tctggacacc gctaacgtcg cagaagtcga acgtctggca cgcatattcc
    26641 ccattgccgg ggtgacaact aacccgagca ttatcgctgc cagcaaggag tccatctggg
    26701 aagttctgcc gcgtctgcaa aaagcgattg gtgatgaggg cattctgttt gctcagacta
    26761 tgagtcgtga cgcgcagggt atggtggaag aagcgaagcg cctgcgcgac gccattccgg
    26821 gcattgtggt gaaaatcccg gtgacttccg aaggtctggc cgccattaag atgctgaaaa
    26881 aagagggtat tactacgctt ggcaccgcag tgtacagtgc tgcacaaggg ttactggccg
    26941 cgctggcagg ggcaaaatac gttgctccgt atgttaaccg cgtagatgcc cagggcgggg
    27001 acggcattcg tacggttcag gagctgcaag cgctgttaga aatgcacgca ccagaaagca
    27061 tggtgctggc ggccagcttt aaaacgccgc gtcaggcgct ggactgttta ctggcaggat
    27121 gtgaatccat caccctgccc ttagatgtag cgcaacaaat gctcaacacc cctgcggtag
    27181 agtcagctat agagaagttc gaacacgact ggaatgccgc atttggcact actcatctct
    27241 aaaggagcaa ttatggaccg cattattcaa tcaccgggta aatacatcca gggcgctgat
    27301 gtgattaatc gtctgggcga atacctgaag ccgctggcag aacgctggtt agtggtgggt
    27361 gacaaatttg ttttaggttt tgctcaatcc actgtcgaga aaagctttaa agatgctgga
    27421 ctggtagtag aaattgcgcc gtttggcggt gaatgttcgc aaaatgagat cgatcgtctg
    27481 cgtggcatcg cggagactgc gcagtgtggc gcaattctcg gtatcggtgg cggaaaaacc
    27541 ctcgatactg ccaaagcact ggcacatttc atgggtgttc cggtagcgat cgcaccgact
    27601 atcgcctcta ccgatgcacc gtgcagcgca ttgtctgtta tctacaccga tgagggtgag
    27661 tttgaccgct atctactgtt gccaaataac ccgaatatgg tcattgtcga caccaaaatc
    27721 gtcgctggcg ctcctgcacg tctgttagcg gcgggtatcg gcgatgcgct ggcaacctgg
    27781 tttgaagcac gcgcctgttc ccgtagcggc gctaccacta tggcgggcgg caagtgcacc
    27841 caggcggcgc tggcactggc agaactgtgc tacaacactc tgctggaaga aggcgaaaaa
    27901 gcgatgcttg ccgccgaaca gcatgtagta acccctgcgc tggaacgcgt gattgaagcg
    27961 aacacctatt taagcggtgt tggttttgaa agcggcggac tggcggcggc tcatgcggtg
    28021 cataacggcc tgaccgctat cccggacgcg catcactatt atcacggtga aaaagtggca
    28081 ttcggtacgc tgacgcagct ggtactggaa aacgcgccgg ttgaggaaat cgaaaccgta
    28141 gctgcattta gccatgcggt aggtttgcca ataactctcg ctcaactgga tattaaagaa
    28201 gatgtcccgg cgaaaatgcg aattgtggca gaagcggcat gtgcagaagg tgaaaccatc
    28261 cacaacatgc ctggcggcgc gacgccagat caggtttacg ccactctgct ggtagccgac
    28321 cagtacggtc agcgtttcct gcaagagtgg gaataaccta ctccaaactc ccggcttgtc
    28381 cgggagtttg aacgcaaaat tgcctgatgc gctacgctta tcaggcctac gcaatctctg
    28441 caatatattg aatttgcgtg ctttttaggc cagataaggc gttcacgccg catccggcac
    28501 tctgtgccag taatctgaaa ctcctacgtt gttcaggagt tttttatccg cttctggaca
    28561 ttttctctac agaacaatcg ttagcccgga aggtcgaatc tgtgactctt attttcacgg
    28621 acgaaccaga tgaaagcttc actggcgctg ctcagcctgc taacggcatt taccagccac
    28681 tccttaaaat ctcccgccgt tccaccaacc gtggtacaaa tccaggccaa taccaacctt
    28741 gccatcgcag acggtgccag acagcaaatc ggcaacacgc tattttacga tccggcgtat
    28801 gtgcggctta cctatcctgg cggtgatgtc ccgcaagaac gcggtgtatg ttccgatgtg
    28861 gtgatccgtg cattgcgcag ccagaaggtc gatttgcaga aactggttca cgaagatatg
    28921 gcgaagaatt ttgccgagta cccgcaaaaa tggaagttaa agcgcccgga cagcaacatc
    28981 gatcaccgtc gggtgcctaa tctggaaacc tggtttagcc gccacaacaa aacacgcccc
    29041 atcagtaaaa atgccggtga ctatcaagcg ggcgatattg tctcctggcg attagataac
    29101 gggctggcgc atattggggt ggtgtcagat ggcttcgccc gcgacggtac gccactggtg
    29161 atacataaca ttggcgcagg cgcgcaggaa gaagatgtgc tgtttagctg gcggatggtg
    29221 gggcattatc gttattttgt taaataagcg atcgccccac atgaagaaaa aattcagatg
    29281 ctttttacac tgacagcttc ttttttctta cggctcacca gcgccagtgc cagcacaatc
    29341 agcacaatac cgctaccttc aacggccccc ggattctcac cgagcagcca ccaggaaaag
    29401 agtacgccgc aaacgggaac ggccagcgtg cttaagctgg caatactggc aggcaagttt
    29461 ttcaatacaa acaaccataa gctccacgcc agcgccgtcg ccagaatcgc actgtaggcc
    29521 agcgcccaga acacggtggg ctgccagtca atttcacgtt gcggcaccag taaagcgacc
    29581 acactcatca ccagcgccgc atagagcatc tgccaggatg ttaacgataa taaatccacg
    29641 cgcggatgac gggtgtatag gcgtttagca acaatcgcgc tcgcccccca actaacaccg
    29701 gagaggattg ccagcatggc acttttcatc gaagagaaat cgagttgcca cggctgcaac
    29761 accaaaaata agccgaaagc ggcaatcaga atcgcgaaat attgcccacg tcgcaggcgt
    29821 tcaccgagaa acaacgcggc gaaaatcacc acccagaacg gcatggtata gctcaggatc
    29881 gccaccttcc ccgcacctcc gctgaccaac gcccactgcg ccagaccaac catcccgcag
    29941 gtttgtaaca gggcaatggc taaggtgtat ttaaacggcg tcgggtgcat tccgcgacca
    30001 cgtaataaaa ggacgatgaa taaaacgaga gcgccgaaaa tgcagcgtaa ggcggtaaag
    30061 tcgaaggcac cgatgtaact ggtgacttgc ttcatgaaaa tccagctata actccagata
    30121 agtgtgagca caaccaggcc actgattgcc agtgggttgc tctttcctgc ggcagacatg
    30181 agagatccag tgtgtagttt tcctgtggac actatacgcc ggtaaagaaa cttcagcgac
    30241 tgcctgaaag acagtcactg aagaaggtca gattacagca ggtcgaaacg gtcgaggttc
    30301 atcactttca cccatgccgc tacgaagtct ttaacaaact tctcgtgggc atcgctgctg
    30361 gcgtaaactt ccgctaccgc acgcaggaca gagttagaac caaataccag atccgcacgg
    30421 ctggctgtgt atttcacttc gccggtttcg cggtcacgtc cttcgaacag ctctttcgat
    30481 tcgtcagtcg ctttccactc gtaacgcata tccagcaagt tcacgaagaa gtcattgctc
    30541 aatacgccaa cgcggtcagt gaagacgccg tttttgctgc tatcgaagtt ggcacccagt
    30601 acacgcatcc cgcccaccag cgcagtcatt tccggcgcgg tcagcgtcag ttgctgtgct
    30661 ttgtcgatca gcagtgactc ggtggtggaa acgtccagac gagcgcgata gttacggaaa
    30721 ccgtcagcaa ttggctccag cagctcaaac atctcaatgt cagtctgatc ctgacgcgca
    30781 tcaacgcgac ccggcgcaaa cggtacatga atgctcaaac ctgcggcgct tgcggctttc
    30841 tcaacaccaa ccacaccagc cagcacgatg atatccgcca gcgaggcttt accagactct
    30901 ttctggattt tctccagaac aggcagagca cgaacggctg cggcgttcac atcccagtcg
    30961 cgctgcggca ttaatgccag acgcgcaccg ttggcaccac cgcgtttgtc gccaccacgg
    31021 aaggtagaag cagatgccca ggccaccgat accagctcac taacagacag accagaatcc
    31081 gcaatcgcga atttcagatc gataatgtcc tgctcggtcg ggttgtagat cggctgcggc
    31141 agcggatctt gccagatcag atcttctttc ggcacttccg gcccgatgta gcgagatttc
    31201 ggccccatat ccctgtgcgt cagtttgaac caggcacggg caaaggcttc gttgaacgcc
    31261 tgcggatcgt tgaggaaacg acgagagatc ttctcgaact caggatcaaa acgcagcgtc
    31321 aggtcggtca ccagcattgt cggtttacgt ttcttcgacg gatcgaacgg atccgggata
    31381 atttccggtg cgtctaccgc ttcgaactgg attgctccag ccggactgcg ggtctgtacc
    31441 cactcatatt tgaacaggtt ctcgaagaaa tagttgctcc actgggtcgg cgtctgggtc
    31501 cagacaactt ccagaccaga ggtaatggca tctgcgccaa cgccgctgcc gtaagtgctc
    31561 gcccagccta aaccttgttc ttcaatcggt gcagcttctg gatcaggacc tacatttgat
    31621 gtcggaccgg caccgtgagt tttacccagc gtatgaccac ccgcaatcag cgccacggtt
    31681 tcttcgtcgt tcatgcccat gttgccgaag gtcgcacgga tagctgctgc cgcagaaagc
    31741 ggttcgccgc tgtgatccgg gccttccggg ttaacgtaaa tcagacccat ctcggttgca
    31801 cccagcggtg ctttcgccag cgcttccgga tgacggtgag tcagccaggc tttttcatca
    31861 ccccagttaa catccagatc cggttcccag acgtcttcac gaccggcacc aaaaccgaag
    31921 gtacggaagc cggagttttc cagcgccacg ttacctgcga ggataaacag gtcggcccag
    31981 gagattttct gaccatattt ctgtttgatt ggccacaaca ggcgacgcgc tttatcaagg
    32041 cttacgttat ccggccagga gttcagcggt gcaaaacgtt gctgaccacg acccgcgccg
    32101 ccgcgtccat cgattgaacg gtaagtacct gcgccgtgcc aggccatacg aataaacaga
    32161 ccggcgtagc tgccccagtc ggctggccac cacggctgag attctgtcag cagggctttc
    32221 agatcttttt tcaggccgta gtaatctaat ttgctgaatt ctttgcggta gtcaaagtcc
    32281 tcacccagcg ggttagaacg attagaatgt tggtttaaca ggtcaacacg gagttgattt
    32341 ggccaccagt cgcgagtggt tgtgcccgcc cccgcgctct ggtcgtgacc gccctgatgg
    32401 aacgggcatt tgccagtggc tgtcgtgtta tggatatcgt ctgacgtgct catcaatgtg
    32461 ctcccctcta cagtgttacc gttacgatac acagcgttag agagaagtta taattgaatt
    32521 aatccacgga tgcgatagtt gagatcttac atattgttgg ttaaagagat gtaggtcaaa
    32581 ttgatcgtaa ttaagctttt ttgattatgt ccgaattcgg acaaatgctt tataaaaaag
    32641 gggtttagct tcatatcctt caagtaatga gggagaaaac aaggctgaca accttatttt
    32701 cccgccctca tttcgaggca gcattttgtg ctctgtttaa aatttgtgat cactgtgtga
    32761 ttttcacaaa agccacacta tttataaacc aggtcgaaca cccagcgtat ggcaaatcgc
    32821 gtaactcatt tcagcgcggt taagcgtata gaagtggaaa tctttcactc cttcacggct
    32881 taaaatcttc accatatcca tggcaatatt cgcgcccacc agtttgcggg tttcggcatc
    32941 atcatccagg ccgtcgaaca tttgcgccat ccacgccgga atacgcacgt tggtcatatc
    33001 ggcaaatttc ttcgcctgtt taaagttaga taccggcaaa attcccggaa taatttccac
    33061 atcaatgccc gccgatacac agcggtcacg aaaacgcagg tagctttcga catcgaagaa
    33121 gaactgagta atcgcgcggt tggctccggc atcgactttg cgtttcagat taagcaaatc
    33181 cgcctgagcg ctttttgctt ccgggtgaac ttccggatac gccgccacgg agatatcgaa
    33241 atctgccact tcttttaata gcgtcaccag gtcagaagca tacatttctg gcttaccact
    33301 tcccggcggc agatcgccac gcagcgccac gatatgacga ataccgttat tccagtagtc
    33361 gcgtgcaatg gtgcgcaact cgtcgggcgt cgcatcaatg caagtaagat gcggtgccgc
    33421 ttccagacca gtgcgatctt taatgccttt aataatgctg tgcgtacggt cgcgctcgcc
    33481 ggagttcgcg ccataggtca ccgatacaaa cttcggtttc aggctgctaa ggcgatcgat
    33541 ggagttccac agggtctgct ccatttcact ggtacgcggc gggaaaaact cgaacgaaac
    33601 gttaatctgc ccctggactt ctgccaggcg ctgattcagg gcatcccgct ggctggcgtg
    33661 aaaaaagctc ataccttacc tcatcaatcg cttgtagtta tatgttgtgt tgtgcacatc
    33721 tatccgttta gacgtccaga tgtaaagatg aaggaaaagc cgaagggcgt caaccgaaaa
    33781 atgaccaatg atggtgagga aaaatcaggg aagatgaaaa aacttcagga tggtaagaaa
    33841 aagaaaatcc ggtatgcaaa gcaaaccgga ctgcaaatat aaactaacca tgtacttatt
    33901 gcttcttgcc ggattcgtcg taaacgctta atccggccta atcctatgat aattcaatat
    33961 atttcattag aaaccataaa cccgaaaaca tgagtaccgg gcattattaa atttctgaaa
    34021 ttacaacaac tgtgccagcc ggttgatatc cgactgaatc gccccggcgg tgacgtcgcg
    34081 ccccgcgcca ggcccacgga tcaccagcgg gttgtcgcga taccagcggc tttcgatggc
    34141 aaagacgtta tcgcacggca gcagtgatgc cagcggatgg tcttcacgca ccgcctccac
    34201 gcccacacgc gctttgccgt tggcatcgaa acgcgccacg tagcgcagca ccagccccat
    34261 ttcgcgggcc gcttccagcc gttgcaccat ctgctcgtta agttcatcgc cattttcaaa
    34321 gaaatggtcg atgctgccgc cttcgcaatg agcaggcacc agcgattcca cacgtacctg
    34381 gtccggttca atgttgtaac ccgcttcacg cgccagaatt accagcttgc gcattacgtc
    34441 tttgccggag aggtcgtcac gcggatcagg ctcggttaag ccctgctgcc acgcctgatc
    34501 caccagctcg gtaaacggca cgctaccgtc gaattgcagg aacagccagg agagcgtgcc
    34561 ggagaagatc ccgctgatcg acaaaatagt atcgccgctg tcgatcagat cgcgcacggt
    34621 gtggttgatc ggcaagcccg caccgacggt ggcattgtac agccagtgac gcccggtttt
    34681 ttcgaaggcg tcgtggatct ggcgatattt attgctgtcg ctggctcccg ccagtttgtt
    34741 ggcgctgata acgtggaaac cgtggctggc gaaatcaaga tactgatcag caagctgctg
    34801 gctggcggta acgtccagca ccactaaatc atcatacgga tgggcgcgca tccacaggaa
    34861 caacgactct tcatcctgct caaccgcttc atcgttgaag aaggctaacg cgcggctggc
    34921 gtccagcccg tcatagctca acaggctacg gcggctgtcc accacacctg ccagcacaaa
    34981 ctcaaagccg gtacgtgccg aaagcgtgct ctgctcacgg gcgaacagtt ccagccaacg
    35041 ggaaccgata ttgcccttac cgaacaatac caggccgatg cgtttttctg cgcggaagac
    35101 ggactgatgc agcccctgaa tcaggctttc ggtcgggccg gtgcgcagta ctgccaccag
    35161 gctgatgccg tcatcggact gccaggtaaa ttcgaccggc tggcctttca gttgctgcca
    35221 gaagcggtgg caatgcagcg ggttacgggt gacgcctgca ccgaccatcg ccaccagcgc
    35281 cagcccctga cgcaggcgca gttcgccagg taatcccgct tcgtcgagga ttttcagcgc
    35341 actgtcggcc acttctgagg tgtagcaaaa ttgcagcaac tggcgatcgt tatgtacgcc
    35401 aaccgccagc gggcgtacct gcgcgcgttt caggatttgg tcgatctctt tatgcgccag
    35461 tttgaaatcc tgactggcgg gcacctgaaa ctcaatcaaa cagacatcat cgtggctggt
    35521 gacaatacgc gcaccagtac cggaggccag cacgcgttca atgcgcgtgg aaccttgatc
    35581 cggcgtgtag ctacagcgca gttgcaggtc gatttcgctg ccagaaaccg gctgtaaagt
    35641 acgggcgtga agaacgggag ccgccaggcg cgccagttcg ctggcctcat ccagacgcag
    35701 caacggcagc aggcaggcat ctttcacttt acgcgggtcg gcactgtata ccccggcgac
    35761 gtcgctccag atggttacgc gagaaacacc cgccagcgca ccgatttgtg tcgcggaata
    35821 gtcggaaccg ttacgcccca gcagcaccgt ttcaccggcg ttgttgcggc tgataaatcc
    35881 ggtcaccacc agacgtttgc ccggatgttg caccagcagc tgttgcagca acgggtaaga
    35941 aagcccttca tcaacctgcg gttgtgcggc gcgttcagcg cgtaaaaact cgcgggcatc
    36001 aagccaggcc gctggcagcc cttgttgatt aagtaccgca gacatcaggc gtgccgacca
    36061 tacttccccg tggcccacca cttccgcata cactgcgtcg ttaataccgc tgtcgagcag
    36121 cgccgccagg cgctcaaggt cgctgacaaa agcgctaatg aggctatcag cttcttcagc
    36181 gggtagcaga ccgctaatca gatcgcactg atagcgacgc agcgtttgtt gaacctgatg
    36241 cgcagagaga cgatcggtct ggcttagttt caaccagtta atcaactggt tagtggtgct
    36301 accggcggcg gaaaccacca tcatatcgtc aggctgagag tactccgcca taatgcccgc
    36361 aacacgcaaa taacacttca catcagccag actactgcca ccaaatttat gcagttgacg
    36421 acctttcgcc cctgcctgcg caatcacact catttttacc ccttgtttgc agcccggaag
    36481 ccattttcca ggtcggcaat taaatcttcg ccatcttcaa taccggtgga gatacgcagc
    36541 agcgtctcgg agatcccggc ggcagcacgc gcttctggtg ccatgcctgc atgtgtcatg
    36601 gttgcggcgt gagagattaa actttccact ccccctaatg attccgccag cgtaaacaac
    36661 gacagcccgc ccaggaaacg acgcagcgtc tgctcatcgc catccagttc aaaactcaac
    36721 attgcgccaa agcctttttg ctggcgcgcg gcaatttcat gcccctgatt ttccggcagc
    36781 gacgggtgat acagtttttt caccaacggc tgggtttgca gatatttcac aatcgcctgc
    36841 gcgttgcgct gggccagctc catacgcggc accagcgttc gcaacccgcg tagcagcaga
    36901 tagctgtcaa acgcgccgcc cgtcacgcca atattgtttg tccaccaggc aagttcagtg
    36961 acaacgtccg ggtctttagc aatcaccacg ccagccacca cgtcagagtg accgttcaga
    37021 tatttcgtac atgaatgcaa caccagatcg gcacccaatg ccagcggatt ttgtaatgcc
    37081 gggcttaaga aggtgttatc caccacgctc accgccccga cttcccttgc cagatggcag
    37141 attttcgcaa tatccacgac gcgtaacaat ggattacttg ggctttctac cagtaccagt
    37201 ttaggttttt ctgccagcgc tgcccgtaat gcctgttcat cgccttgatc aacaaacaac
    37261 acgcgatagc aaccgcgttt cgccagactg tcgaacaggc gatagctacc gccgtagcag
    37321 tcgtgcggcg caaccagcag atcgccaggt ttcaaaaaga cggtcgttac caagtgaatc
    37381 gcggacatgc cggtattagt aagtactgcg ccagcaccgc cttccagttc tgccagcgca
    37441 cgctgaacca catcgcgcgt tgggttgccg cgacgtgagt aatcatgcgc gcgcggttca
    37501 ttaaatccgg taaagttata ggtgctggaa agatggatgg gagggacaac gcaaccatac
    37561 tgttcgtcgt catttaaccc gctacgcact gcgatggtgg cctgtttacg cgtcatgtga
    37621 tgaagtttcc tgggctttgt cggtgaaatg tcaggcacca gagtaaacat tgtgttaatg
    37681 gacgtcaata catctggaca tctaaacttc tttacgtata gattgagcaa atcccaaata
    37741 gccgttaaaa ttatatgcat tatcacgccg acaggtgcat tacacgatgt cacggtaacg
    37801 cctgtacggt aaactatgcg ggtttacggt cagtacccac ataaattgtg tggtctggtc
    37861 tcaatttatt gacgaagagg attaagtatc tcatggctga atggagcggc gaatatatca
    37921 gcccatacgc tgagcacggc aagaagagtg aacaagtcaa aaagattacg gtttccattc
    37981 ctcttaaggt gttaaaaatc ctcaccgatg aacgcacgcg tcgtcaggtg aacaacctgc
    38041 gtcacgctac caacagcgag ctgctgtgcg aagcgtttct gcatgccttt accgggcaac
    38101 ctttgccgga tgatgccgat ctgcgtaaag agcgcagcga cgaaatcccg gaagcggcaa
    38161 aagagatcat gcgtgagatg gggattaacc cggagacgtg ggaatactaa agcaaaaaag
    38221 agcggcgcgg agtggaatcg cctgatgcgc tacgcttatc aggcctacgt catattgcaa
    38281 tttattgaat ttgcacgaac ttgtaggccg gataaggcgt tcacgccgca tccggcataa
    38341 acaacgagca cgttgtctgc gacccaccgc tttttataca tggacgttta actatgaaaa
    38401 acaggctgct gatcctcagc ctgctggttt ctgtacctgc ctttgcctgg cagccacaaa
    38461 ccggcgacat catctttcag atctctcgct catcgcaaag taaagcgatc caactggcga
    38521 cccattccga ttatagccac accggtatgc tggtgatacg caacaaaaag ccctacgttt
    38581 ttgaagcggt cggcccggtg aaatacaccc cgctcaagca gtggatcgcc catggtgaaa
    38641 agggcaaata cgttgttcgc cgcgttgaag gcggactgag tgttgaacaa cagcaaaaac
    38701 tggcgcaaac ggtaaaacgt tatctcggta aaccgtatga tttcagcttc tcatggagcg
    38761 acgatcgcca gtactgttcg gaagtggtgt ggaaggttta ccagaacgcg ctgggaatgc
    38821 gcgtgggcga gcagcagaag ttgaaagagt tcgatctcag caacccgctg gtccaggcta
    38881 agctcaaaga acgctacggc aaaaatattc cgcttgagga aacggtagtt tcaccacaag
    38941 ccgttttcga cgcgccacaa ctcaccacgg ttgccaaaga gtggccgctg ttttcgtggt
    39001 aatggatgat gaaaaaaata attcccgccg tagtctgttt gttgctttgt agttgcacga
    39061 cctttccgca aactgtcgct ccggtaaacg gtgatgcctc caattagttg aattaaggat
    39121 ataatactcg cttttaaggt tgtttcatgg tcacagttaa ccttcattgc ccccgttgcc
    39181 agtccgttca ggtttaccgt cacgggcaga acccaaaaag acatgatcga ttccgcggcc
    39241 gtgactgtca ccgggtattt cagctcagtt actgttacga gccccgtaaa ccgggagtta
    39301 aagaccaaat cgctgaaatg actttcaacg gtgccggggt tcgcgatact gcccgtacgc
    39361 tgaaaatcgg cattaataca gtcatccgaa ctttaaaaaa ctctcgccac gaagaataac
    39421 gtcatccccg gtcgctcatg ctgatgtggc actcatctgc gaacttagtc agcaatggag
    39481 ctttgtcggc agtaaagcgc gacagcactg gctctggtac gtgtacaaca cgaaaactgg
    39541 tggtgttctg gcctacactt ttggcccccg gactgacgaa acctgtcgtg aattgctggc
    39601 gctgcttacc cttttaccat cggcatgttg accactgacg aatggggtag ttacaccaga
    39661 gagttaccga aggaaaagca tctgactggt aagattttta ctcagcgcat tgagcgcaac
    39721 aatctgaccc tgagaacccg aatcaaacgt ctggcccgca gaaccatctg tttctcgcgc
    39781 tctgtagaac ttcatgaaaa agtcatcggt gcttttatcg aaaatatatg ttctactaat
    39841 tggagtcatc acccttctga tgagctttcc ttggatgatt accagactga atacacagcc
    39901 aaagattttt acctcattct gctctttttt ctcaaatgac atatattata ttttacatca
    39961 tattcatagg catatgacat tcccccgccc ccattatgtc attgtgggtt gatgttgggc
    40021 tttcataaaa aaaacatctt tttttgaact agcattaatt atatttactt tatatattaa
    40081 aatattttct ccatctggaa atattttagc cacacaatat aaagcagctg aatccgaatt
    40141 acttaaaaat atattatcat tacccataac tccagccatt aaattactgg agactaaagc
    40201 aaggaaataa caacattgta ttgccttcat tttaattact cccttgtctg ataattgtaa
    40261 ctccattccc tttagctttg ggattatatt ttagataggt gttatttggt gttcccaagt
    40321 aactacttgt attctttttt tcccattcca ttcatataag aattcgttaa atctttgctt
    40381 gaaaaattta gactatcata aacatcattt tcttttgtta ctttatttcc tttatcgtca
    40441 gagtaaaaac cgtgagtgtg ataatcgcct actctctctg aaccttcagg gcattttata
    40501 tttcgcaagt ctacactatc attatcattt gaacttatcg gattcatggg gaaatattca
    40561 ccttgcttct tacatattag tccaccatat tctttattct cgttaattga ttctccatta
    40621 cacat
//
LOCUS       DAJTNN010000012        40049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTNN010000012 DAJTNN010000000
VERSION     DAJTNN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.012"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(447..2138)
                     /gene="fruA"
                     /locus_tag="OO150_000603"
     CDS             complement(447..2138)
                     /gene="fruA"
                     /locus_tag="OO150_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5514852.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAVARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2155..3093)
                     /gene="fruK"
                     /locus_tag="OO150_000604"
     CDS             complement(2155..3093)
                     /gene="fruK"
                     /locus_tag="OO150_000604"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5514853.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(3093..4223)
                     /gene="fruB"
                     /locus_tag="OO150_000605"
     CDS             complement(3093..4223)
                     /gene="fruB"
                     /locus_tag="OO150_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5514854.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4591..5772
                     /gene="setB"
                     /locus_tag="OO150_000606"
     CDS             4591..5772
                     /gene="setB"
                     /locus_tag="OO150_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5514855.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRTKD
                     V"
     gene            complement(5769..6023)
                     /locus_tag="OO150_000607"
     CDS             complement(5769..6023)
                     /locus_tag="OO150_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5514856.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6178..6750
                     /gene="yeiP"
                     /locus_tag="OO150_000608"
     CDS             6178..6750
                     /gene="yeiP"
                     /locus_tag="OO150_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5514857.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6973..8439
                     /locus_tag="OO150_000609"
     CDS             6973..8439
                     /locus_tag="OO150_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5514858.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMNGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8557..9543
                     /gene="yeiR"
                     /locus_tag="OO150_000610"
     CDS             8557..9543
                     /gene="yeiR"
                     /locus_tag="OO150_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5514859.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWTVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9582..10295
                     /gene="lpxT"
                     /locus_tag="OO150_000611"
     CDS             9582..10295
                     /gene="lpxT"
                     /locus_tag="OO150_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5514860.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10707..11273
                     /gene="mepS"
                     /locus_tag="OO150_000612"
     CDS             10707..11273
                     /gene="mepS"
                     /locus_tag="OO150_000612"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5514861.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11454..13010
                     /locus_tag="OO150_000613"
     CDS             11454..13010
                     /locus_tag="OO150_000613"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5514862.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGGETPEQARWLSEPGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            13092..14906
                     /locus_tag="OO150_000614"
     CDS             13092..14906
                     /locus_tag="OO150_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5514863.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQQLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14907..16001
                     /locus_tag="OO150_000615"
     CDS             14907..16001
                     /locus_tag="OO150_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5514864.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            16001..17026
                     /locus_tag="OO150_000616"
     CDS             16001..17026
                     /locus_tag="OO150_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5514865.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            17028..18617
                     /gene="yejF"
                     /locus_tag="OO150_000617"
     CDS             17028..18617
                     /gene="yejF"
                     /locus_tag="OO150_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5514866.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKLQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18621..18965)
                     /locus_tag="OO150_000618"
     CDS             complement(18621..18965)
                     /locus_tag="OO150_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514867.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19298..20481)
                     /gene="bcr"
                     /locus_tag="OO150_000619"
                     /pseudo
     CDS             complement(19298..20481)
                     /gene="bcr"
                     /locus_tag="OO150_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20509..21204)
                     /gene="rsuA"
                     /locus_tag="OO150_000620"
     CDS             complement(20509..21204)
                     /gene="rsuA"
                     /locus_tag="OO150_000620"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5514868.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21353..23113
                     /locus_tag="OO150_000621"
     CDS             21353..23113
                     /locus_tag="OO150_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5514869.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23238..23522
                     /gene="rplY"
                     /locus_tag="OO150_000622"
     CDS             23238..23522
                     /gene="rplY"
                     /locus_tag="OO150_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5514870.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23661..24668)
                     /gene="yejK"
                     /locus_tag="OO150_000623"
     CDS             complement(23661..24668)
                     /gene="yejK"
                     /locus_tag="OO150_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5514871.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24850..25077
                     /locus_tag="OO150_000624"
     CDS             24850..25077
                     /locus_tag="OO150_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5514872.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            25097..26857
                     /gene="pbgA"
                     /locus_tag="OO150_000625"
     CDS             25097..26857
                     /gene="pbgA"
                     /locus_tag="OO150_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5514873.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRPQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26932..27008
                     /locus_tag="OO150_000626"
     tRNA            26932..27008
                     /locus_tag="OO150_000626"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26966..26968,aa:Pro,seq:ggg)
     gene            27227..28468
                     /locus_tag="OO150_000627"
     CDS             27227..28468
                     /locus_tag="OO150_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5514874.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28964..29170
                     /locus_tag="OO150_000628"
     CDS             28964..29170
                     /locus_tag="OO150_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR5514875.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLAWQKEREQEARGTAA"
     gene            29185..29850
                     /locus_tag="OO150_000629"
     CDS             29185..29850
                     /locus_tag="OO150_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514876.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVYDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSCIAMRRNTSHHGADSFYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSFS
                     SGEIRILNCGDCPSPLGLLSRLIIDKWSPIELAFPLLGGHLNTRALKKAKPRKCHYHS
                     QGF"
     gene            29876..30076
                     /locus_tag="OO150_000630"
     CDS             29876..30076
                     /locus_tag="OO150_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5514877.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            30066..30287
                     /locus_tag="OO150_000631"
     CDS             30066..30287
                     /locus_tag="OO150_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514878.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            30280..30513
                     /locus_tag="OO150_000632"
     CDS             30280..30513
                     /locus_tag="OO150_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514879.1"
                     /translation="MRDDRFNSLKQEFPGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            30506..30739
                     /locus_tag="OO150_000633"
     CDS             30506..30739
                     /locus_tag="OO150_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514880.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            30745..31044
                     /locus_tag="OO150_000634"
     CDS             30745..31044
                     /locus_tag="OO150_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5514881.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            31041..32441
                     /locus_tag="OO150_000635"
     CDS             31041..32441
                     /locus_tag="OO150_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5514882.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPVEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVISIDGDDVAPL"
     gene            32641..32892
                     /locus_tag="OO150_000636"
     CDS             32641..32892
                     /locus_tag="OO150_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514883.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            32889..33299
                     /locus_tag="OO150_000637"
     CDS             32889..33299
                     /locus_tag="OO150_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR5514884.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            33310..33582
                     /locus_tag="OO150_000638"
     CDS             33310..33582
                     /locus_tag="OO150_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5514885.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            33821..34027
                     /locus_tag="OO150_000639"
     CDS             33821..34027
                     /locus_tag="OO150_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514886.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            34027..35082
                     /locus_tag="OO150_000640"
     CDS             34027..35082
                     /locus_tag="OO150_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5514887.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQSDGKKWSEHDKDIYDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAEMACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            35094..35429
                     /locus_tag="OO150_000641"
     CDS             35094..35429
                     /locus_tag="OO150_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5514888.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLVDNGEFK
                     PWDGQESGKAVYLTSYLVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            35442..35855
                     /locus_tag="OO150_000642"
     CDS             35442..35855
                     /locus_tag="OO150_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5514889.1"
                     /translation="MATNESIMALPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQTVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            36017..36451
                     /locus_tag="OO150_000643"
     CDS             36017..36451
                     /locus_tag="OO150_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514890.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMAGFLAGWRHAVSVRRASMEQSADTEQ"
     gene            36732..37013
                     /locus_tag="OO150_000644"
     CDS             36732..37013
                     /locus_tag="OO150_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514891.1"
                     /translation="MTEAEILGLIRRAGGISQQTDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            complement(37692..>40049)
                     /gene="yejO"
                     /locus_tag="OO150_000645"
     CDS             complement(37692..>40049)
                     /gene="yejO"
                     /locus_tag="OO150_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5514892.1"
                     /translation="NGGYQYIEMNGTAEYSVLNDGYQIVQMGGAANQTTLNNGVLQVY
                     GAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGGAIKASTRVM
                     EAFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTATG
                     VDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAIDTIL
                     DEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANVWAGT
                     MVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSV
                     WAIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNTLSGN
                     GNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDAAFTL
                     GNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDTV
                     RERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESSTI
                     RGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGKM
                     SNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRADV
                     GNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNHVKVNDDGKFNNDVAG
                     TPGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT        10073 a        10186 c        10021 g         9769 t
ORIGIN      
        1 tacgacataa tccttttcgc gcatagcaca tcctctggta aaaaattacc aaccctgtca
       61 atccacaaga atatagatac tgcaaagacg gtttaaacat gtgttcatat ttaaacatgt
      121 gctcatagca tgcatgtttt acgtaaagta aattaccgcc agagaggcaa aatgggcaga
      181 tttgagcagg ctcacaaaat gcgattaaat gcctgaatta caaaagcgag gaatttgtga
      241 cgggatgcac agaacgggaa caataaaaaa ggcagaaaat aatctgccct tgaggaaata
      301 accttagtgt ttgatgaacg tttcaagttt ctactgaaag ataaaaccat ttaaattcat
      361 aaaattacat tcacaaaccg gtcattctct ttgcgctcat caaatgttac agggcaggaa
      421 atttctgccc tgtaacacac cttttattac gctgctttcg ctactgcgtc cacttccgga
      481 cgtttcagga aggcataggc caaacccgcc accagcgtac cggcaataat tgctaccagg
      541 taacccagta ccggcgtaat agcgccaggg atcagcagaa caaacagacc accgtgcggt
      601 gccatcagtt tcgcaccaat cgccattgag attgcgccag tcagcgcccc acccacgata
      661 cagcacggca gcacacgcat cggatcacga gcagcaaacg gaattgcacc ttccgaaatg
      721 aagcacagtc ccaataccag agcggctttg ccaccttcct gctgcgcttt gtcgaatttg
      781 cgacgcgcca ccattgttgc cagacccatt gccagcggtg gcaccatacc tgccgccata
      841 atcgccgcca tcgggccata ggtttgagta ctcagcagac ccacaccgaa tgcgtacgct
      901 gctttgttta ccggaccgcc catgtcagta cacatcatgc caccgaggat cgcccccagc
      961 agaaccgcat tcgcagtccc catggtctgc agccagtgag tcagcccttc gagaatgcca
     1021 gcaactggtt taccgatcag gtagatcatc gccagaccga ccaccagact ggaaattagc
     1081 ggaatgatca ggatcggttt cagcgcctcc atactctgtg gcagtttcag ttgcgtactg
     1141 attaacttcg caatgtaacc agccaggaag ccagcaatta taccgccaat aaagccagaa
     1201 ccggtgctga ccgccagcat accgccaatc agacccggcg tgaggcccgg acgatcggca
     1261 atggagaagg caatataacc tgccagtacc ggcaccatca gcgcaaaggc tgaaccaccg
     1321 ccaatctgca tcagcgccgc tgccaacgta cccggctctt taaacgcttc gataccaaaa
     1381 gcaaaagaaa gcgcgataca cagaccacct gcgacgacca tcggcagcat ataagagacg
     1441 cctgtcagca agtgacggta agcgcctgca ctctctttct taccttcagt ggtcgccgtt
     1501 tgagctttgc ccgccggttc atacggcgtt gcttcagcaa ccgctttatc cagttcctgc
     1561 gcggttttct tcagcgccag accggtagag gtacgataca tcggtttacc agcaaatttc
     1621 gccagatcca cttcgatatc tgccgccaca atcaccagat ccgctgctgc gacttcttca
     1681 ggggtgattg cattgcccgc gccaacagaa ccacgggttt caactttcac ccaccagcca
     1741 cgttttttcg cttcggtttc aatggcttca gccgccataa aggtgtgtgc tacgccagtc
     1801 gggcaagcag tcaccgcaac tacgcgtttc ggaccgctgg cagcaaccgg tgctgtcgca
     1861 gcgaccggcg cagtgtaagg tttcgcatgg cctttggctt cactcaggaa cagctcaggg
     1921 tgcgcaactg cccgggaaat atcacccagc cagacatttt taccgttcag cgcgctgtca
     1981 ttcgggatgg aatcaccgag aacaatcgcc atttctgcgt cgttcggatt gtcgatgatt
     2041 tccagttttg cttttcgcgc caccgcgccc agcagggtct tcgccatata ggcgcgtgcc
     2101 tgaccgagat tagcgtcaat aatcagcagc gttttcatta tgcctctcct gctgtcagtt
     2161 aaaaggttgt aagtcgacgc gcgccatcat tgcggccaac tgcggacgat cggtaatacc
     2221 cacattgctt tgacttaccg ccagggctgc aacagctgtc gccagacgca gtgtgtgttc
     2281 actggattca cgcatcagca agccataaat caggccacca accatagaat cccctgcgcc
     2341 aacggtgctt acgacatcga ctgacggtgg tttggcgatc cattcgccgg aggcattaac
     2401 ccaaagcgcg ccttcggcac ccagtgaaat aacaacatgc gcaatacctt gttcacgtag
     2461 cgcatgtgca gcttcaatca catctttcat ttcaggcagt ttacggcctg cccaaatttc
     2521 caactcgcgg cggttaggtt tcaccagcca cggtgccgct ttcaaacctg ctactaacgc
     2581 ttcacggcta ctatcaaaga taatgcaagg acactgacta cgcaggcgag tcatccagtc
     2641 ggtgaacgct tccgggctga cgcctgacgg taagcttccg ctgacacaga ccatatcgaa
     2701 ctgaccgagc cagctcagag aatcagtcac aaagcgttcc cagtcggcgg gggtgacttc
     2761 aaaacccgag aagttgaagt cggtcacttc gccgtctttt tccgtcagct taacgttaat
     2821 tcgggtgcgc ccctgtacaa cctggaaacg gttggcaatg cccagctcgc tgaacagttg
     2881 ctgaaaacca tcctgattgt ctttacccag gaagccgcca acggtgacat caattcccag
     2941 gtcttttaat actttggcca cgttgatgcc tttacccgcc gcatgcagac cggtggtttt
     3001 caccaggttc acttcgccgc gttcaatttc cgggcagaaa ccaacaaggt cataagccgg
     3061 attaagggtg atcgtagcaa cacgtctgct cattatgcgc cctccccaag accagcagcg
     3121 atagcgtcgc caatggcttt cagcgcctgt tcagcatctg caccctgggc ggtaaagcgt
     3181 aggcgatgac ctttcttaac gccaagtgcc acaactttca tcagactacg tccgtttgcc
     3241 ggtttgccgg tgccatcaag gtttgtcacg gtaatatcac tgttaaattg tttaatggta
     3301 ttgaccagca tggtacctgg acgagcatgc aggccgtgtt cattgcgcac cacaaactcc
     3361 gcgcttaaca cgtcgtcggt cggcgcatca tcgctggtca gcagcgccag caacgttgcc
     3421 gcatctgctt tcagcaagcg gtcagcttta ttgtcgagca ataaatcagc gagacgctta
     3481 agaaccgcga tgggctgatc gtcattcatc gccacactca ccagcatggc tgccgtttcg
     3541 ccgtccacag caaaagcatt tgccgcacgg cttaccgcaa tcgcgctacg cagattgcct
     3601 tcggcgctat cgctcagcca gataccctgt ccgagattca gcggttgttc attgatggct
     3661 ttggtgacga aagtggcgtc aactgccccc gcctctttca gacgcgcagc gttcagcgcc
     3721 tgaagagtca gcagatcgct ggcgacgata tccagcgtca gcatttcgtt gtcgagcttc
     3781 agctgctcac tctgcttttc gcccatcagt aatgcgcgaa gttcttctgc tgttgttgct
     3841 gacttcagtt gttcagcaac ggaatcatcg ctcagtacgt gggtcagctg gcgtagcagg
     3901 cccagatgtt catccgagct ggcagcaata ccgattgcca cgtacgctac ctgaccgtca
     3961 ccccaggtga cgccttccgg gaactgaaat acctgaacgc cggttttcag cacctgatcg
     4021 cgggtgtcgg tagtgccgtg tggaatagca ataccattgc cgaggaacgt tgaggtttgc
     4081 tgttcgcgcg ccagcatgcc attgacgtag ccttctgcta cattaccggc ctgcaccagc
     4141 gccgcagcga cctggcgaat cgcctcttct ttgtctccgg ccttttcgcc cggatggatg
     4201 tcctgtacgg ataactggaa catagttctc ctctcttgct gaattgaaac gattcagcct
     4261 ctatgagaaa aaaagcgcca acctggctta gggttaaaga caagatcgcg ctgaaacgtt
     4321 tcaagaaagc ataatacttc tgtttcagca cgcaaggaaa gccgtaaagt tatgagcaaa
     4381 agtttgatgt gctgcacatt tttttgtcat ttttcctaat tgctgatggg aaaatccgtt
     4441 atcagcaatt tcatttcagc agcgtcagca gccaatcagc aacagtgaaa tggcattttg
     4501 attattcgtg gcgcttttga tttcgctgtc tgtttatttt ctgacaagca gcgtaaactc
     4561 cgcgtcttcc tcttccagtg atagacctgc atgcataact cccccgcagt ctccagcgcg
     4621 aaatcgtttg acctgacctc gacggcgttt ttaatcgttg cctttctcac cggtattgcg
     4681 ggtgctctgc aaaccccgac actcagtatt ttccttaccg atgaagtaca tgcccgtccg
     4741 gcgatggtgg gattcttctt taccggcagc gctgtcattg ggattctggt aagtcagttt
     4801 ctcgctgggc gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt ttgctgcctg
     4861 ttaggcgtgc tggcctgcac cctttttgcc tggaatcgca actactttgt tttgctattc
     4921 gttggcgtct ttcttagcag ctttggctcg accgctaacc cgcaaatgtt tgcccttgcc
     4981 cgtgaacatg ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt tttacgcgct
     5041 caggtttcac tggcatgggt cattggccca ccgctggctt atgcgttagc gatgggtttc
     5101 agctttacgg taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg cgtgatggtg
     5161 tggctgtttt taccgtcgat gcaaaaagag cttccgctgg cgaccggcac ggttgaagcg
     5221 ccgcgccgta accgtcgcga tacgctactg ctgtttgtca tttgtacatt gatgtggggc
     5281 tcgaacagcc tgtacatcat caacatgccg ctatttatta tcaacgaact gcatcttccc
     5341 gagaaactgg ccggtgtgat gatggggacc gccgccgggc tggaaatccc gacgatgttg
     5401 attgccggat atttcgccaa acgtctgggt aagcgtttct taatgcgcgt tgctgccgtg
     5461 ggtggcgtct gtttttacgc aggaatgctg atggcgcatt cacctgccat tctgttgggc
     5521 ttgcagctgc taaatgctat ttttattggc attctgggcg gcatcgggat gctctatttt
     5581 caggatttga tgcccggtca ggcaggttca gccaccacgc tctataccaa cacgtcgcgc
     5641 gtgggctgga tcatcgcagg atcagtggcg ggcatcgtcg ccgagatctg gaattatcac
     5701 gctgtgttct ggtttgcgat ggtgatgatt atcgccactc tgttttgctt actgcggact
     5761 aaagatgttt aagacgcggt cagcatttcc agatcgataa gccaggtcat ggcctgctga
     5821 cgcgagttac cgcacatttc agcgctggct tgtaagcctg cacagacttt cggtcgcagt
     5881 ggcgaggtga aaattttaca gcgttgctgt tcatcaagct gaatgcaggg cgtattggcg
     5941 ggcttgccat ctggcatacc gggaataggg ctgaaaattg aaggggcagt gcaacacgcc
     6001 ccgcaacccg gacggcattc catacgttgt tctcttaagg ttaaccaccg gattgatgag
     6061 cgcgcacagt accacctttt ttgcaccagc aaaagtgcga ataccacttg cccgaaaggc
     6121 ccgtcgcgag tactttgtcg cgatattttt gacattttcg actacaggaa tttttcgatg
     6181 ccaagagcga acgaaattaa aaaaggtatg gtactgaatt acaacggcaa actgctgctg
     6241 gttaaggata ttgatattca gtcgcccact gcccgcggtg ccgctacgct gtacaaaatg
     6301 cgtttttctg atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg tgatgatatc
     6361 gttgacaccg tgacgctgac ccgccgttac gttgacttct cctatgtcga tggcaacgaa
     6421 tatgtcttta tggataaaga agactatacc ccgtatacct tcaccaaaga tcagattgaa
     6481 gaagagttgc tgtttatgcc agagggcggc atgccggaca tgcaggtgct gacctgggac
     6541 ggtcaactgc tggcgcttga gctgccgcag accgttgatc tggaaatcgt tgaaaccgca
     6601 ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt gagcactggt
     6661 ctggtgattc aggtaccaga atacttaagc ccgggcgaaa aaattcgtat ccatatcgaa
     6721 gaacgccgtt atatggggcg tgctgactaa cttcagccgc atgcagaaaa gggatagctc
     6781 aggctgcccc ttttttaatt tattatacca atcttctatt ttgcgcttcc tgaacacctt
     6841 acgccctcaa ttttcactcg ttgattgatc gccctcacac ttcatcgcat taacaatcca
     6901 gaccaatttc aattgctgtc atataacttt acactgtcat tgttaattta tcgttactaa
     6961 gacgtgactc ctatgaagac aattgcctcc gttacgctcc cgcatcatgt acacgctcca
     7021 cgctacgatc gccagcagtt gcaatcacgt atcgttcatt ttggctttgg cgcctttcac
     7081 cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg cccagggcgg cgactggggg
     7141 atctgtgaaa tcagcttgtt cagcggtgat caactgatga gccagctccg cgcacagaac
     7201 catttatata ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat tgtcggtgcc
     7261 gttcacgaat gccttaatgc aaaactggat tccttagcgg caattattga gaaattttgc
     7321 gagccacagg tggcaattgt ttccctgacg attaccgaaa aaggctattg tattgacccg
     7381 gccaccggtg cactcgacac cagtaatccg cggattattc acgatctaca aacccctgaa
     7441 gaacctcact ccgcaccggg tattctcgtc gaagcactga aacgccgccg tgagcgcggc
     7501 cttacaccgt ttaccgtgct ctcctgcgac aatattcccg acaatggcca tgtggtgaaa
     7561 aacgcggtgc tgggaatggc agaaaaacgt tcgccagaac tcgccgggtg gataaaagaa
     7621 cacgtcagtt ttccgggaac catggtcgac cgcattgttc cggctgcaac cgacgaatca
     7681 ctggcagaaa tcagccagca tctgggggta aatgatccct gcgcgattag ctgcgaaccg
     7741 tttatccagt gggtggtgga agataacttc gtcgctgggc gtcctgcctg ggaagtcgca
     7801 ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg tatgcttaat
     7861 ggcagccact cttttctcgc ttatctgggt tacctttcag gattcgccca tatcagtgat
     7921 tgcatgcagg atcgcgcatt tcgccatgcc gccagaacat taatgctgaa tgagcaagcg
     7981 ccgacactac aaattaaaga tgtcgattta acacaatatg cggacaagtt aattgctcgt
     8041 tttgctaatc cggcgctgaa acataagacc tggcaaatcg caatgaatgg tagccagaaa
     8101 ttaccgcaac gtatgctggc aggtattcgc atacatctgg ggcgcgaaac ggactggtcg
     8161 ttgctggcat taggcgttgc tggctggatg cgttacgtca gcggcgttga tgatgccgga
     8221 aatgccattg atgttcgcga tccgcttagc gataaaattc gcgaacttgt tgcgggcagc
     8281 agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg tgatgatctg
     8341 ccagataacc cgcattttgt gcaggccatc gaacaagcct ggcaacaaat cgtacaattc
     8401 ggcgcacatc aggcgctatt aaacaccctc aaaatttaac gatttctgcg gttaaagcgg
     8461 atgaagctca ccttcgtccg ctctcccctt ctcttttctg ccttttttag ccaggattaa
     8521 cgctcagtta acttaccaga gtattgcgga gccgttgtga ccaggaccaa cctcatcacc
     8581 ggttttctcg gcagcgggaa aaccacgtcg attcttcatc tgttagccca taaagatccc
     8641 aacgaaaaat ggacggtact ggttaatgaa tttggggaag tcggaattga tggtgctttg
     8701 ctcgccgata gcggcgcatt gctgaaagag atccccggcg gctgcatgtg ctgcgttaat
     8761 ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc agaccgcttg
     8821 ttgatagagc cgaccgggct gggccatccg aaacagatcc tcgatctgtt aaccgcacca
     8881 gtctatgaac cgtggataga tctgcgcgcc accttgtgca ttctcgatcc acgcttgctg
     8941 ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc agctggctgc cgcagacatc
     9001 attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct acagcgttgg
     9061 tggcagcaaa atggtggcga tcgacaatta attcacagtg agcatgggaa agttgacggt
     9121 catcttctgg atttgccgcg tcgcaattta gccgagttgc ccgccagcgc cgcgcattct
     9181 catcagcatg tcgtgaaaaa agggttagca gcgttaagcc tgccagagca tcaacgctgg
     9241 cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg gctggatatt cgacgctgat
     9301 acggtattcg acaccattgg cattctggaa tgggcgcgac ttgcaccggt ggaacgcgtc
     9361 aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca accgtcaggg cgatgacctg
     9421 cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat ttccagcagc
     9481 gaagctgact ggaatgcctt gcagagcgcg ctgttgaagc ttcgtttagc gactaccgcg
     9541 taaggttgcc tgcgtttttc agtaagataa ttagagaaaa tatgattaaa aatttgccgc
     9601 aaatagtgtt gttgaatatt gtcggcctcg cgctgtttct ttcctggtat attcccgtta
     9661 atcatggatt atggttgccg attgatgcgg atatttttta tttctttaat cagaaactgg
     9721 tcgaaagtaa ggccttttta tggctggttg cattgaccaa caatcgcgct ttcgacggtt
     9781 gttctctgct ggcgatggga atgttgatgc tgagtttctg gctgaaagaa aacgccccag
     9841 gcagacgacg tatcgtgatt attggtctgg taatgctatt aactgcagtg gtattaaacc
     9901 agctgggtca ggcattaatt cctgtaaaac gggccagccc aacattgacg tttactaata
     9961 ttaaccgcgt cagcgaactg ctctctgtcc ccacgaaaga tgcctcacga gatagctttc
    10021 ccggcgatca cggcatgatg ctgcttattt tttcggcatt catgtggcgt tatttcggta
    10081 aagttgcagg tcttatcgcc cttattattt ttgtggtttt tgcgtttccc agagtaatga
    10141 ttggcgcaca ctggtttact gacatcattg tcggttcgat gaccgtgata ttgatcggtt
    10201 tgccctgggt gttgctgacg ccattaagtg atcgattaat cacctttttt gacaaatcac
    10261 taccgggaaa aaacaaacat ttccaaaaca aataactcac agtaattaac atcatcaggt
    10321 ttatttttat agtgaggata atcctgatga tgcgcaccgt gctttcatct atcgaacgca
    10381 aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt cactcaatcg tactttaatt
    10441 gaccaatccc gcttattaac tttctgtatc actttttctt ataaaaaatc atgtaaaacc
    10501 gctcgccaag accgcaccaa tcgggtaatc tcgaactcgt tttgcctcgg cggtagatta
    10561 tcctcacagc atataatttt gtgcgttagt ccacagattt ggccttaagg aattgtttca
    10621 acatgcccag gtaattagtc tcgtgtcgct tggcattttt ttataacgat atttgtcgtt
    10681 aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg agatatatct
    10741 tgcgcgggat tcccgcgatt gcagtagcgg ttctgctttc tgcatgtagt gcaaataaca
    10801 ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca tcactgcaag
    10861 cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg cgaattatgg
    10921 atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact aaaaaaggta
    10981 tcgattgttc tggtttcgta cagcgtacat tccgtgagca atttggctta gaacttccgc
    11041 gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat ttgcgtacgg
    11101 gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt tatatcggca
    11161 acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc atgaatgaac
    11221 cgtactggaa gaagcgttac aacgaagcac gccgagttct cagccgcagc taataaaccg
    11281 tttggatgca atcccttggc tatcctgacg agttaactga aagcactgct taggtagtgc
    11341 ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca ggctatagtc
    11401 cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga atcatgttca
    11461 tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgtagca ggcgtgatga
    11521 ttgcgatact ggttagctgc cttcagtttt tagtggcctg gcataagcac gaagtcaaat
    11581 acgacacact gattatcgac gtacaaaagt atctcgatac ctattttgcc gacctgaaat
    11641 ccactactga ccggcttcag ccgctgacct tagatacctg ccagcaggct aaccccgaac
    11701 tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg aaagataaaa
    11761 aaacattctg ttcatctgcg actggtgaga tggacattcc actcaatgaa ttgattccgg
    11821 cgctcgacat taataaaaac gtcgatatgg cgatcttacc cggcacgccg atggtgccga
    11881 acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc ggtgtgtttg
    11941 ccgctctgaa tctcaacctg acgccttctc tcttttatag ttcacggcag gaagattacg
    12001 atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca cgtttgatga
    12061 acgttaatga attaaccgac atgcctgtcc gtgaaactaa aattgcgggc attcctctga
    12121 ccgttcggct ttatgcggat gactggacat ggaacgatgt gtggtacgca tttttactgg
    12181 gcggcatgag tggaactgtc gttggcctgc tctgctatta cctgatgagc gtacgtatgc
    12241 gccccggcag agaaatcatg accgccatca agcgcgaaca attttacgtg gcgtatcaac
    12301 cggtggtgga tacacaagct ttgcgagtaa cgggcctgga agtactgcta cgctggcggc
    12361 atcctgtcgc gggagaaatt cccccggatg ccttcattaa ctttgccgaa tcgcaaaaga
    12421 tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc gcagaattag
    12481 aaaaagtgct gccagtaggc gtcaaatttg gtattaacat tgcgccggac catctgcaca
    12541 gcgaaagctt taaagcagat atccagaaac tgctcacttc cctgcccgca caccatttcc
    12601 agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc acacaactct
    12661 tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc accgggcaca
    12721 gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac cgtggattta
    12781 tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg ctgacgctgg
    12841 cgaaacgcct caatatgctg acggttgctg aagggggcga aacgccggaa caggcgcgat
    12901 ggctaagcga acccggcgtt aatttcatgc aaggctactg gattagtcgc ccgttaccgc
    12961 tggacgattt tgttcgctgg ctgaagaaac cgtatacgcc gcagtggtaa ggtgtgctta
    13021 cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga gtaaaaggaa
    13081 atccgttgca gatgattgtg cgcatactgc tgctgtttat cgccctgttc accttcggtg
    13141 cgcaggcgca ggctatcaag gaaagctatg cctttgccgt gctgggcgaa ccccggtacg
    13201 catttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt gggcagataa
    13261 cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta tgcactgcgc ggcaacccgg
    13321 gcgcacgcac cgagcagttg tacgacacgc tatttacgac ttccgatgac gaaccaggca
    13381 gttattaccc gctgattgct gaaagtgcac gctatgctga cgattattcc tgggtggagg
    13441 tcgctattaa tccgcgcgcc cgttttcatg atggttcgcc cattactgcc cgcgatgtag
    13501 agtttacttt tcaaaaattt atgaccgaag gcgtgccgca atttcgtctg gtctacaaag
    13561 gcaccaccgt caaagccatt gcaccgttaa ccgtgcgcat tgagttagct aaacccggca
    13621 aagaagatat gctgagtctg ttttcgctgc cggtatttcc agaaaagtac tggaaggatc
    13681 acaaacttag cgacccgctc gccacgcctc cgcttgccag tggtccgtac cgcgttacgt
    13741 cctggaaaat ggggcaaaat attgtctatt cccgtgtgaa agattactgg gcagcaaact
    13801 taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat tacctcgatg
    13861 ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg gaaaacgacg
    13921 ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac atcatcaaag
    13981 atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat atccaacgtc
    14041 cggtattcag cgatcgccgg gtccgggaag caatcactct cgcctttgac tttgaatgga
    14101 tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa cagttatttt cagaataccg
    14161 aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca ccaatgaaaa
    14221 aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct aaaggcgatg
    14281 gctacgatcg tgacaacctg ttaaaagccg acacacttct caacgaagcg ggctgggtgc
    14341 tgaagggtca gcaacgcgtt aatgccacaa cgggtcagca actcagcttt gaattattgc
    14401 ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg caacggctgg
    14461 gtatcaacat ggacattcgc aaggtggata actcgcaaat caccaaccgc atgcgcagtc
    14521 gcgactatga catgatgccg cgcctatggc gggcgatgcc gtggcccagt tccgatttac
    14581 agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc gtgcaaagcc
    14641 cggttatcga ctcactgatc aaccaaatta ttgccgcgca gggaaataaa gaaaaattat
    14701 tgccgttggg gcgagcactg gatcgcgtat taacgtggaa ttattacatg ctgccaatgt
    14761 ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccacccc gctgtacgcc
    14821 ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg gccaaactgc
    14881 cgtcagccag gcaacaggga gagtagatgg gcgcttacct gattcgccgt ctgttgctgg
    14941 tgatcccaac attatgggcg attatcacca tcaacttttt catcgtgcaa attgcgcctg
    15001 gcggtccggt cgaccaggcc atcgccgcca ttgagtttgg taatgccgga gtattacccg
    15061 gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc aatatcagcg
    15121 acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgctgag atcactcatc
    15181 gctacggttt tgataagccg atccacgaac gttacttcaa aatgctctgg gactacatcc
    15241 gctttgattt tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg attaaagaca
    15301 gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat ctggtgtcga
    15361 ttccgttagg cattcgcaaa gctgttcata atgggagccg ctttgacgtc tggagtagcg
    15421 catttatcat tatcggctac gccattccgg cctttttgtt tgccatcctg ctgattgtct
    15481 tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt tccgctaact
    15541 ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc acgctgccgg
    15601 tgctggcgac ggtgattggt ggctttgcgg cgctgaccat actgacaaaa aactcattcc
    15661 ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta agtgaaaaaa
    15721 atattctctg gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc ggttttccgg
    15781 cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg ttttcactca
    15841 atggtctggg cttactgggc tacgaagcga ccgtctcgcg cgattatcct gtaatgtttg
    15901 gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt gatatcagct
    15961 atacgctggt tgatccgcgt attgattttg agggacgtta atgtcgcgac tcagccccgt
    16021 caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc ggctactggt cgttatggat
    16081 tttcctcgtc ttgtttggtt tgagtttgtg ttctgagctt atcgccaacg ataaaccatt
    16141 gctggtacgt tatgacggca gttggtattt cccgttgttg aaaaactaca gcgaaagcga
    16201 ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga aacaacggct
    16261 ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca gtatcaactt
    16321 tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg gaacggatgc
    16381 caacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg ttctgtttgg
    16441 cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc tacaaggcta
    16501 ttacggcggt aaagtcgatc tctggggaca acgctttatt gaagtatggt cggggatgcc
    16561 gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct ggtggctgct
    16621 ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg cggagttttt
    16681 acgtactcgt aatttcgact acattcgtgc ggcgcaggcg cttggcgtca gcgatcgcag
    16741 tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct ttttaccgtt
    16801 tattttatgt agttcgatca ccaccctgac ctcactcgat ttcctcggct tcggtctgcc
    16861 gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc ttcaggctcc
    16921 gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc tgatctttat
    16981 tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg acgcaaactc
    17041 tgttagcgat tgaagatttg tcggtgggtt ttcgccatca gcaaaccgta cgtacagtag
    17101 tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg ggtgagtcag
    17161 gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc ccgccggttg
    17221 aatatctctc cggcgatatt cgttttcgtg gcgaatcgct gcttcatgcc agcgatcaaa
    17281 cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaagagccg atggtgtcgt
    17341 taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc caccgcggga
    17401 tgcgtcggga agcggctcga ggcgaaattc ttaactgcct tgatcgcgtt ggtatccgcc
    17461 aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa cgccagcggg
    17521 tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat gaaccgacca
    17581 ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa ctgcaaggcg
    17641 agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga aaactggccc
    17701 accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaattacgcc gctacgctat
    17761 ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg tcaggcgatc
    17821 cagtgccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt caggttgcct
    17881 tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg gtgaaaaaca
    17941 tcagttttac gctacgagcg ggtgaaacac tgggtttagt gggcgagtcc ggttccggga
    18001 aaagtacgac gggactggcg ctgctgcgac tgattaattc tcagggcagc atcatctttg
    18061 acggtcagcc actgcaaaat ttaaatcgcc gccagctgtt acctattcgt catcgcattc
    18121 aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt ttgcagatta
    18181 ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc gaacaacaag
    18241 tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt tatccggcgg
    18301 agttctctgg tggtcagcga caacgtattg caattgccag ggcattaatt cttaagccct
    18361 cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag gcgcaaatat
    18421 tgacgctatt gaaatcattg caacaaaagc tgcaactggc ctatttgttt atcagccacg
    18481 atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa ggggaagtag
    18541 tggaacaagg accgtgcgcg cgcgtgtttg ccgcaccgca gcaggagtat acgcgtcagc
    18601 tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc ggcaattgcc
    18661 acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt tacaaacagg
    18721 cacggctcac cttcacactc cagcaccgaa cacggaactt cgatgtcgct taacgcctgg
    18781 cttagtttgt acatcaccga ccatgcatta tcaccatccg ggctaagcaa tttaagcgcc
    18841 accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt cgaacctgcg
    18901 aaaccagcag accagcgaaa gttctgcggt agacgatgga cgattgagag ttgtaatgaa
    18961 gtcacttatt ttccccggaa gcacatttac ttcacaattt gtttacatca attttaacac
    19021 atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct gcgtggcctc
    19081 tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc tatatgtacc
    19141 cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt acataaattg
    19201 attttacata aaataaacat atatcggggg aatgatagat ttgtggttga catgcatcaa
    19261 caaaggaagc cttttagctt cctcgttgtg caatagatca ccgttttttc ggtcgactgg
    19321 cgtacagaca gaagagaatg gagctgcttg cgcagaatgc aattgaccaa atcatcggcc
    19381 atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct atgccaaaac
    19441 ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca tcaagaatga
    19501 ccgccatcgc attggatgac accatcgaca cacagcccac aaacgccgca acgccaacca
    19561 ccagaatccc agccccagca gcgcactgat gaccatccac gctgccataa taaattgtat
    19621 ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cgaacgaagc ggctgttaaa
    19681 gatggtcatc acgaacagga aaacaatgtt tagtgcaaag taataaccaa agttttccgg
    19741 cgcgacgtgg ttaatttcaa tgtagacaaa cggtccggcg ctcaggaacg agaacattcc
    19801 ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac ggaacagcgc
    19861 cgcaaagtta ccaatagtag tacgaatgtg aaaaggctgg cgacgctccg gtggtaaggt
    19921 ttctttgatc agaaagaaga tcattgccga agccagaatc gccgccacgg cgaggatcca
    19981 gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg gtgccatcag
    20041 cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact cttctttcgg
    20101 gtagatatca cgcatcaaag cgttgataac catgctggcc gcagccgcag ccagcccgtg
    20161 gaaaaaacgc atcacaatca gctgatcgat ggtttgtgcc aacgcacacg ccaccgcggc
    20221 ggcggcaaac accagcgtac cgccgagcac caccggctta cgcccgaagc tgtctgccat
    20281 cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactga gggtcatctg
    20341 cgtactgccc gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg gcagatacat
    20401 atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa tagcaaacga
    20461 cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt agacgacgct
    20521 ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag catccagcgt
    20581 aataccgcca atacgttcac gatgcagctc aaccacgtgg ttacccacgg cggcgaacat
    20641 gcgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg ttggggtaat
    20701 cacttccagc accgcaggct tagtgagatc tttttcgtta tgcagctgca cgcctttagc
    20761 aaattgctct gccgtatcgt cagctacagg tgattccagt gtcaccagat aggtcttctc
    20821 gcaatgatgg cgcggagaag taatgcggtg cgaccactga ccatcatcag tcatcagcac
    20881 cagaccggtg gtatcgatat ccagacgtcc tgcggcgtgc agtttccagg ctaccggttc
    20941 atccagaaaa tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa catagccctg
    21001 aggcttattg agcatgaagt aacgtggacc atgttgctgc gccagcgggt tgccatcgta
    21061 agcgacatca tgttcaggaa gcagtttgaa cgctgcatta cgaacaattt cgccatcgac
    21121 ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc cgagttgctg
    21181 tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaacgg aggtcgggca
    21241 ttgacccgaa aatctgaact gttgtctgcc cagtatagcg gtctaataac atccctcaag
    21301 ggaaaaagat ccatggcata ctattagcag aataatctac ctacgcgaga ccatgatttt
    21361 tacactccgc ccatatcagc aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca
    21421 taaaacccct gccgttatcg tgctgcccac cggcgcaggt aaaagcctgg tgattgccga
    21481 actggcacgg ctggcgcgtg gtcgtgttct ggtgctggca cacgtcaaag aactggtggc
    21541 gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc gatatttttg ccgctgggtt
    21601 aaagcgcaaa gagagccacg gtaaagtggt atttggcagc gtgcagtcgg tcgctcgtaa
    21661 tcttgatgcc tttcagggtg aattttcgct gttgattgtc gatgaatgcc accgtattgg
    21721 tgacgatgaa gagagccagt atcagcaaat cctcactcac ctgactaaag tgaatcccca
    21781 cttacgcctg ctggggctga ctgccacgcc ttttcgattg ggcaaaggct ggatctacca
    21841 gttccattat cacggcatgg tacgcggcga tgagaaagcc cttttccgtg actgcattta
    21901 tgagctgccg ctgcgttata tgattaaaca cggctatctg acgccgccag aacgactgga
    21961 tatgccagta gtgcaatacg atttcagccg cttgcaggca cagagtaacg ggctgttcag
    22021 cgaagctgat ctcaaccgtg agctgaaaaa acagcaacgc attacgccac atatcatcag
    22081 ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg attttcgccg ccaccgttga
    22141 acacgcaaaa gagattgtgg gattactgcc tgccgaagat gcagcactga ttactggcga
    22201 cacccccggc gctgagcgcg atgtgttaat tgaagatttt aaagcccagc gttttcgcta
    22261 tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc
    22321 cattctgcgt cctaccgaat cagtgagtct ttaccaacaa attgtcgggc gcggtctgcg
    22381 tctcgctcct ggcaagactg attgcttaat tcttgattat gcgggtaatc ctcacgacct
    22441 ctacgcgccg gaagttggta caccgaaagg caaaagtgac aacgtcccgg tacaggtttt
    22501 ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa acgaccgccg acgggacatt
    22561 gattgaacac tttggtcgtc gctgtcaggg atggtttgaa gatgacgacg gccatcgcga
    22621 acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcgg aaaacgatat
    22681 tgccgcccgc cgctgccgcg aatgtgacac ggtactggtt gatccggatg atatgttaaa
    22741 agcggcgcta cgactgaaag acgcgctggt attacgctgt agcggcatgt ctttacagca
    22801 tgggcacgac gagaaaggcg aatggttgaa aatcacctat tacgatgaag acggcgcgga
    22861 tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcga actgccttcg agcagctttt
    22921 tatccgcccg catacgcgca caccgggcat ccctctgcgc tggatcaccg ccgccgatat
    22981 cctcgcccag caagccttac tgcgacatcc ggattttgtc gtcgcccgca tgaaaggtca
    23041 gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga
    23101 attacgcggt taatggtagt tttgattgat gtataaggcg attgagtata gaatctcgcc
    23161 cgcttttgca tacgcaaagc agatcactta cctgttgctg ggtcgcctgt agcaggatta
    23221 atttaagaga gaaagaaatg tttactatca acgcagaagt acgtaaagag cagggtaagg
    23281 gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc aatcatctac ggtggcaaag
    23341 aagcaccgct ggctatcgag ctggatcacg acaaagtcat gaacatgcaa gctaaagctg
    23401 aattctacag cgaagttctg accatcgttg ttgacggtaa agaaatcaaa gttaaagctc
    23461 aggacgtaca gcgtcacccg tacaaaccga agctgcagca catcgacttc gttcgcgctt
    23521 aattgctgaa taagttgtaa aaaaccccgc tccggcgggg ttttttgtat ctgcagatta
    23581 tgcctgatta cggtattgct attttttgca ggccagataa ggcgttcacg ccgcatctgg
    23641 cattcagaat acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa ttggtcgcgc
    23701 aaattcggcg gcgtgccttt aatggtcaag gtatcggttg ctggatccca gaagatgcgt
    23761 tcacctaaca gcatcgcatc aaagttaata gtcaagccac cgccgctacc ggcaaacttc
    23821 gtcaactgac gcagtgtgct acggtctgcc gggaagctct cttccagttc gtagcctttt
    23881 tcagccgcaa acttcgtgaa gctcacttcg cttacgcctg ccagctcttt cgacagcgat
    23941 tccagttcga tctcttcgcc tgcctgcagc tgctcattac agtagctata aacctgctgg
    24001 cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc atcaaccgct
    24061 tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt cgctggcccc gaggaaatcc
    24121 ataaagaaat ccgccacttt gcgccctact cgccctttca ggaaagtgag atagcgggtg
    24181 gactctggat tggtttccca ttcggttaaa tcgatacgcg caacaatatc cgcatgattg
    24241 atatcaagat aatgggttgg gttgatgtcg agattttcgt taacgcgcat actgctcagg
    24301 ttgctcagaa ctgccaccag cagatactcc accgccagat aacggtagtg gcaaaacagc
    24361 acgaatccac cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg cccggttgcc
    24421 gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag cgtctgcgcc
    24481 agttcactct cttcgctaaa caagccgtaa gctttatttt tggcgctata gacccgatgc
    24541 agttctgcca ccatctcaac gacggtttct gtcggttcca gcaatgaatc gcgcaacacc
    24601 agctcaaggt tctgctcatc acgcttgata agctggtgca gagcaatctg gttgatatcc
    24661 agactcatga taaactctcc tttaagaccg ggcggtattc aaccaccgcc ggttaagcga
    24721 cgcaataaaa gatggcggtc attttacaga aggcagcgta acgagaatga tttaacgcat
    24781 aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg ccctttcatc aactaactga
    24841 tttcgattta tgccacaaat ttcccgctac agtgatgaac aggttgaaca attgctcgca
    24901 gagctgctca acgtactgga aaaacataag gctccgaccg acctttccct gatggtgtta
    24961 ggcaacatgg tcactaacct tatcaacacc agcattgccc cggcccaacg ccaggcaatt
    25021 gccaactctt ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc gcactaaggg
    25081 aaacagataa caggttatgg taactcatcg tcagcgctac cgtgaaaaag tctcccagat
    25141 ggtcagttgg gggcactggt ttgcactgtt caatattctg ctttcgctcg tcattggcag
    25201 ccgttacctg tttatcgccg actggccgac aacgcttgct ggtcgcattt attcctacgt
    25261 aagcattatc ggccatttca gcttcctggt gttcgccacc tacttgctga tcctcttccc
    25321 gctgaccttt atcgtcggct cccagaggct gatgaggttt ttgtccgtca ttctggcaac
    25381 ggcgggaatg acgctattac tgatcgatag cgaagtcttt actcgtttcc atctccatct
    25441 taatcccatc gtctggcagt tggttatcaa cccagacgaa aatgagatgg cgcgcgactg
    25501 gcagctgatg ttcatcagcg tgccggttat tttattgctt gaactggtgt ttgcgacgtg
    25561 gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc cgctggccgc
    25621 attcttattt atcgccttta tcgcctcgca tgtggtgtat atctgggccg atgccaactt
    25681 ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt tcgtacccga tgacggcgcg
    25741 acgtttcctt gagaagcatg gcctgcttga tgcgcaggag tatcaacgcc gtcttattga
    25801 gcaaggtaat ccagacgccg tttccgtaca gtatccgtta agcgaactgc gctatcgcga
    25861 tatgggcacc gggcagaatg tgctgttgat tactgtcgat ggcctgaact actcacgctt
    25921 cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca
    25981 tatgagctcc ggcaacacta cagacaacgg catctttggc ctgttctatg gcatctcgcc
    26041 gagctatatg gacggcattc tgtcgacccg tacccctgcg gcgttaatta ctgcgcttaa
    26101 tcagcaaggc tatcagctgg gattattctc gtcagatggc tttaccagtc cgctgtatcg
    26161 ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc ccccaatccg acgagcagac
    26221 cgccacgcag tggatcaact ggctgggccg ctacgcacaa gaagataacc gctggttctc
    26281 gtgggtctct ttcaatggca ctaacattga cgacagcaat cagcaggcat ttgcacggaa
    26341 atatagccgg gcggcaggca atgtcgatga ccagatcaac cgcgtgctca atgcactgct
    26401 tgattctggc aaactggaca atacggttgt gattatcact gccggtcggg gtattccgct
    26461 gagcgaagac gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc cattagtgat
    26521 tcactggcca ggcacgccgg cgcagcgtat taatgcgctg actgatcata ccgatctgat
    26581 gacgacgctg atgcaacgcc tgctacatgt cagcacacct gccagcgaat attcgcaagg
    26641 tcaggatttg ttcaaccctc aacgccgtca ttactgggtc actgcagcgg ataacgatac
    26701 gctggcaatt accaccccga aaaagacgct ggtgctgaac aataacggta aataccgcac
    26761 ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt tgttattgca
    26821 agtgctgaca gacgagaagc gttttatcgc taactgatta attataaatc agttagcgaa
    26881 atatcttact tgcaatcggt gtggaaaacg gtagtattag cagccacgag tcggcacgta
    26941 gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt
    27001 gccgaccaaa tcgcccaccg gataccaccg gaattaacag tttttagaca cgaaaagccc
    27061 gctaatcctt aattagcggg tttttttatg gtttttagtc accggacatc accggattta
    27121 taagctatca gccggatttt ttagttatac gcttagttat acgggcttgc gtataactaa
    27181 agccgtataa ctaaaaccct gttttttcgt gtggaatcat atacatatgg cgagaaaaac
    27241 acccccatta accacggtgc agatcaaagc agccagacca gcggaaaagg aatacaccct
    27301 acaggacggc ggagggcttt ttctcctggt caaaccgtcc ggatcaaaac tctggcgatt
    27361 ttcctactac cggccatcgg acaaaaaaag aatattgctg agttttggat cgcttgatga
    27421 tgtttccctg gctgatgcca gaaaacgccg tagcgagtac agagcgttaa tcagtgccgg
    27481 aactgacccg cagggccatg agaagaaaaa acgagaggca gaggcccgaa gacaagggaa
    27541 cacgttcgaa aatgtggcgg cggcatggta ccaggtgaaa atcagccaga atctggcccc
    27601 caacacgatt aaagacatct ggcgttcgct ggataaatat gtattcccgt tcatcggcaa
    27661 cacgccaata gataccctca ccgcccgaag gttcgttgaa gtgcttacac ccatcaagga
    27721 gcgcggcaac ctggaaacac tcaagcgggt tttacagcgc gttaatgagg taatggatta
    27781 cgccgccaac agtgggctga ttgatgccaa tccggctatg aatgtgcgta aggcgtttcc
    27841 ctcacctgta aaaaaacata tgccaacaat ccgccccgaa cagctgccgg agcttatgca
    27901 ggctttatca gtatcggcaa cagaacggca gaccagatta ctgattgaat ggcagttact
    27961 gaccgtaacc cgtcccgccg aagcgtcatc aacgcggtgg gatgaaatca acctggacgc
    28021 gaagcaatgg acgatacctg ccggacgcat gaagatgcgc agggatcacg ttatcccgct
    28081 ttccggtcag gctatggcgg tgctggaggc catgaaacca atcagccacc accgcaatta
    28141 cgttttccca agtctgaaag acccacagca gccgatgaac agccagacag ctaacgcagc
    28201 attgcggcgt atgggattcg ctggcgtgct ggtgtctcat ggattacgcg ccatattcag
    28261 cacagcagcg aacgaggaag gattcgagcc ggacgtaata gaggcggcac ttgcccacgt
    28321 cgacaccaac gaagttagac gggcatacaa ccggagcaac tacatagaaa aacgcatcgt
    28381 gctgatgcgc tggtggggcg aatttgtcga ggctgcggcg acgggcgtaa ccctcgccag
    28441 tggtaaaagg ggtatccgag ccgtgtagct gtacagaaaa ccagtaaaaa ctacgaaaac
    28501 catgtaaaac cgtcgtataa ttgcatcaaa tttaacgaca aggccgtgaa acatgaaacc
    28561 gttaagatgc aaaaaaatat cagatgcaat tgcgacgggc tgcaactggc cctgatggtt
    28621 cagcatgaat tttggtcaac ctacgatccg gaggacagaa cgacggcccc atcaaaaaaa
    28681 caggtagtag attttctggt atcccgtggc gcttccagaa atctggcggt aagtattgat
    28741 aaggtcgtac gtccggcatc tatgaagatc ggaggcaggc ccaaaaaatg gcggtaacaa
    28801 tcctggaagc ggcagaaatg ccgctttttt tataattcca tttcaaatca tcaatataaa
    28861 aacggtgtat accgtttaaa aacggtggga actgttttta cccatatccg atgatttacc
    28921 gtatttgtca ccggaataca ccggattcac aaggtaaatc acgatggaag caatcagaaa
    28981 aattatcttt cgccaggaag taaaaaaaat tatccacatc aaggcagaca gcacgctgca
    29041 aagcatgatc aacgccggag aatttccgca gggttttcgc gttggtttac gccgtcgcgg
    29101 atggtatgag gatgatgtgt tggcctggca gaaagaacgc gaacaggaag cacgcggaac
    29161 ggctgcttaa cgggtatcac agatatgaca aacacgaaaa aagcggcccc gatatggagc
    29221 cgcctttctg aacaattaac ccgctgcgcc ttatgtgtat atgatcccaa acataagcac
    29281 ggggatgata gccgctatca ggctggtggg caatgcaatc agtctggttc agttcgttgc
    29341 catacctgca atgagcgctt ttccctatac tctttaagga attgctcaag ggcaaaagca
    29401 catggtgcga atctttctga ttcatgctct atctttctgc gccgtctttt ccgtgccggt
    29461 gataatgttt tggtcaattc tttatcggtc attgtgttgt cctgcatagc aatgcgccgt
    29521 aatacctcac accacggcgc tgatagtttt tatcctttgg gttctatgcc gcgccgcttt
    29581 agttcagtgc gccccaactc tttaagccag ttggctaggc ttatgccgtc gctctgtgct
    29641 tctttgtcga attgctcttt tagctctgga gaaattcgca ttctgaattg tggggattgc
    29701 ccgtcccctt tagggctttt atcgcgtttg attattgaca agtggtcacc tattgaatta
    29761 gcctttccat tgttaggtgg ccaccttaac acaagagcac ttaaaaaagc aaagccccgc
    29821 aagtgtcatt accactcgca gggcttctaa ccaacaacgt aaactaggag ccgttatggt
    29881 tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt ggcgttttct
    29941 ggccctgagt gccggagaat ctcaaatcat ccacgtaacc gcctggacgg aacgcgaagc
    30001 gcgtagccgt tgcccgtccg gttgtgttgc tgtattcgcc gcccgtattc gtcaggggga
    30061 aacctatgca caataaaacc acaccgaacg cagcagccgc cgcactcact acgctgatgc
    30121 acgcgcttat tgatattgaa tgcactgcag agcttgcgca gggagaagaa cagaaagacc
    30181 ggacacagtt cgccctggaa tgtatccgat acatcgcaac gcggtcgctg aatgacgcta
    30241 aaaatattct tgttgctgat tgtgaaaatg gggggggtta tgcgtgatga tcgttttaat
    30301 tccctgaaac aggaatttcc cggcgttcct gatgatgcgg ctgatgcgct ttcgtcaatg
    30361 ccagaactta ttagagcggc ttttttctta ctttccacga gagaatataa atcaacgggg
    30421 cttgatgtac tgaatatcgc cgccgattat gcggaatatg tggcagaggc gcgttacaga
    30481 agaaaatttc ctgaggatgt aagccatgcg tgatatttac cacgaaacaa tagaccgcgc
    30541 atttcttgca ctttctcaca gtgaaaacat gctggaaata ttgcgcatat ggcttgaaac
    30601 acttggcgac aatgaacgcg acaaacaaaa atcaagaatt gccacggcat taataacgct
    30661 tcttgagcct gtaataatgg aactgcaaga aatagatcta ttgcacgaca gatataaaga
    30721 acagcacacc ggagaataaa aataatgaaa cttaaatatt ctggcttaac tgccagtggc
    30781 aacactcacc ctaaatttac gcgcggtgat atttaccgcg accagtacgg cggcacggta
    30841 atgattaagg gcgtggaaga acggcgtgta acctaccgcc gtgaaggtta cgaatatgat
    30901 tgcgtgatgc ctgtttatca gttccggcgt gatttttctc tggtacagac cgcgccgcat
    30961 aacgtgccca ccagcaacgc cagggcacgg gcaaacatcc agaagctgaa aaccatgatt
    31021 aacggattca ggggcaagaa atgaaaagcg caccgaactt aaaaaaacag ccttacgaca
    31081 agatgaccga agtcattatt tttgcgggta gtgatgcctg ggcacatgcg aaacagtggc
    31141 aggaacagga cgggcgactg gctggcgata atgtgcctcc cgttgtgctg gctgatgatc
    31201 aactggatga actggcagac ctgagaatca tcgacgaggg gcgctattgt gtccggctgt
    31261 acaaggcagg ccacatcagg ccatcaaata ttaatgccat tgcgcacaag ctggcggcgg
    31321 cgggtgtaac tgatgcgaat tattaccccg aagggatgca cagccatatg cgggagaact
    31381 ggcgcgaata cctggaacgg gtgcgcggga aagagccggt ggaagaaaaa aaccaccagc
    31441 gaaaaaccac gctaccgatg agcgttggat ctaccggata cgacacgcaa ctggattacg
    31501 tggttaaggg gattattccg gcggtatcgc tatgcagcat atacggggct agcgggtcct
    31561 ataaatcatt ccttgccgga tcgtgggcgt gccatgttgc cactggtcgc cagtggggag
    31621 gccgcagggt tgcacatggt gcggttctct atgtggttgg tgaaggcggt atcggcgttc
    31681 cgcgtcgtgt aaaagcctgg gaggttgtgc acgatgagca ggtgaaaaat ctgtatctgg
    31741 taaaccgccc catctttccg gctgccccgc ttgatgttga tgaaatggtt atcgctgccc
    31801 gtcaggtgga gcgggaaacg ggtaaacctg tacgcatgat tattctggat acgctggcgc
    31861 gttgctttgg tgggaatgat gaaaatgatt cccgtgatat gggggcgttt atccgtggtt
    31921 gtgacgaact gaaacgacgc acaggggcca cggtgctggt ggttcaccat tccggcaagg
    31981 atgagacgaa aggcgcgcgc ggttccagtg catttcgtgc ttcgctggat gctgaatacc
    32041 ggatacgcag ggaggacgca ggaagcgaag cgctggttat ctcatgcacc aaaatgaagg
    32101 acgcggagga actcaaagaa gccgcatatg acttacgcgt ggtggagctt tttaccgacg
    32161 ctgacggtga attaatcacg tcgctggtgg tggtggatga tccgcgccct cctgttgaac
    32221 tggagcgcat cgaggaggca gggaacaaga cggaaaacca taccgcgcta tgggggtgca
    32281 tccgttcacg cacacagaac ggcgacaagt gcacgatccc gctgttacgt gatgacatga
    32341 aaaagctggg gtatgaaatg aaaaacttcc ggcgctggct gtacaagctg gaaaaagatg
    32401 gggttattag tatcgatggg gatgatgtag cgccgctata aaagtgagga gcaaaagcga
    32461 gggggataga aagagggcca aaattagccc gctctccctc acttttcgac ctgtatacat
    32521 cctcaaaagt gaggggtaaa aaatacttat aaaacacaca catagaaaaa ccgaaaatcc
    32581 caactgcgac gaagtgagac gcttgaaaaa gtgaggcgaa aaagtgagag gttgcgagaa
    32641 atgacccaaa aacgcagaga cagaacagag ccaaaatata aagcgttaga catgactgag
    32701 cacaccttaa aggtggcaat cagaacgata gaccgccaca cgcgggaagg atacgcgaag
    32761 gaacatcccg acctgataag cgcattcatg accacggcgg cggcaaactt tgccacgctg
    32821 acagaacggg agattgccga agcggaacag gtaacaacca tcaacgttaa aaccggagag
    32881 gtggaattat gacggcacag atagccgctt acgggcggct ggtggacgac ccgcaggtaa
    32941 aacagaccag caagggcaca ccgatgacgc tggcgcgtat ggcggtatct ttgccatgca
    33001 gccaggcaca ggacggacag gcgacgttat ggttatcggt catcgcattt ggcaaacagg
    33061 ccggcttcct ggctaaacat caaaaaggcg atgttgccag cgtatccggc acgatgcagg
    33121 taagccagtg gaccggacag aacggagaaa cgcggcaggg ctggcaggtt atcgcagaca
    33181 gcgtaatcag tgcccgcgcg gcacgtccag gcgggaacag acgcaaaacc acaggcacac
    33241 agggtaatca gccaccagcg ggaggcgatg acccttacgg tgatgatatt tcgttctgag
    33301 ggggtgacga tggtacatga ccgcatagcg gaggaactgg aggcgaaagg cttttaccgg
    33361 agggcggcgg cgcgatgggg tgaagtcatg cagctggtgg agacagacaa ggaacggcat
    33421 cacatcacga tgcgacggct ggaatgttca aggaaggcac agagggcacc ggagccgccg
    33481 gataatttcg gagacctgaa aaaggcagtc gatcgcactt atgccgaaat gggtatagat
    33541 ggtgctggtg atgaaatatg gcgcaattac caggacagct aatcaaacag ccggagaaat
    33601 ccggcttttt tgcgcccaaa aaaagcccga taaggtcaga gggttcttat cgggcttttg
    33661 catatgaggt ttttggatgc actggcgttc gtgatcggga taatcatttc ataatttgca
    33721 acataactca atattattgc ataaaatgca attctgatta taatcaggac tggataaaca
    33781 tccagttatg atttttttag tcgaagagga atttcttact atggctgaag agaaaaaagg
    33841 cggtgtttcg gtgtacataa gccccgaaat cgtggaggtg ctcaagcagc gccacaaaaa
    33901 aaactatgag gctggcgtgg cggctggact ggatccgctg atgacgccgg agccgtcgat
    33961 aggttcactt gtacgctctt atttacttgc ggcgcttggg atgcataaaa attatggggg
    34021 tgaataatgg caggcaaagc aacggcactt aacactaacc agctttttat gtacctgaat
    34081 cgcggggata ttgcggattt taaattcagc cctctgttta ccacgctgtt tttcccgaac
    34141 gtggcgacat tcagcacgca aaacatcatg ctggataccc tggacattga agaagtcacc
    34201 atgtcggcgt tttgttcgcc tatggtgggt agccaggttc agcgcgataa agggtacgaa
    34261 accagcacaa tcaaacctgg ctacatgaag ccaaagcacg aaatcgatcc aacgaaaacc
    34321 atcatgcgca tggctggaga agatccggca cagcttaacg accctaccta tcgccgtatg
    34381 cgcctgatta ctggcaacat gcgccgccag ataaacgcca ttaaggcacg cgtggaatgg
    34441 ctggcggtga atgcggtaac gaccggaaaa aacatcattg agggcgaagg catagaacgc
    34501 tatgaaatcg actggaagat accggaaaaa aacatcatag agcagtccga cggcaaaaaa
    34561 tggtccgagc atgataaaga tatttacgat ccaatctatg acatcgaact atacgcagat
    34621 caggcaggtt gccccgccaa cgtcatgatt atgggcgctg aggtatggcg cacgttacgc
    34681 agctttaaaa aattccgtga actgtacgat ctttcccgtg gttcagaatc cgccgcagaa
    34741 atggcctgta aaaacctggg cgaagtggtg agctttaaag gctatctggg cgatattgct
    34801 cttattgtct attccggcaa atacactgac agcgacggca ccgaaaaata tttccttgag
    34861 cctgatttgc tggtcctggg caacaccaac aataaagggc tggtggccta tggtgcgatt
    34921 atggatcagg aagcggtaag aacgggcgca acgcaaaaca tgtactaccc gaaaaactgg
    34981 attgaggacg gcgatccggc gattgagtac gtgcagacac acagcgcacc gcagccggtt
    35041 ccggcagata ttcgcaaatt tgttaccgtc aaaattggtt aacgggggat tatatggaca
    35101 ctccatatat tgagttattt gcaggcagtc agcaggtatc aacgacactg gtacattttg
    35161 ccgctgatgc tggcgttatt caggaattta ccccgctgat gctggtggac aatggcgagt
    35221 ttaagccgtg ggatggtcag gaatctggca aggctgttta tctgacttcg tacctcgtgg
    35281 acacgtcaaa gcagaaatca gcacagtgtt acaagacggg gatattcaat attgccgccg
    35341 ttaactggcc tgagagcgtc gacaccgatg cgaaaaaatg cgccgccttt gcgggttctg
    35401 gcgtatccgt tcagccgctg gcccgataag cagggggaac gatggcaacg aatgaaagca
    35461 tcatggcgct accgctggcg agtaaattta aagccgaagc gcgggcaatg gctgacagag
    35521 gtttatcaac ctacgaggcc gtatatcaac tcaacaaact ggaagagcag gacaagccgc
    35581 gcgctgatgc gattatggcg cttcatgagc ataaagacta tcagccgctg ttgcgtgcaa
    35641 tggcaaacgt gccttgtatc gatgttgata cggctaaaaa catcctgaac atgaccatag
    35701 agcaggaacg cccgaaggtt gcaccagagc ttaccgcagc ctttgaaaac tttatggaca
    35761 tgcacagccc acaaaccgta tcagctggca tggcatacga tggcagaaac ccaggcgatg
    35821 acggcgacat cgatcgcata ctgaaaacca tctgagacaa ggccggagaa atccggcttt
    35881 tttgcgggtc cttcctggaa ttatggcccg ttacggggcg gcgacctcgc gggttttcgc
    35941 tatttatgac gtttttccgt gaaggtgaca ccaccaccac ttgattaata tttaaccatg
    36001 cagttaaggt aacattatga ttgataaagc ttgttttgta agtcagcagg aaatagctga
    36061 acatttcaag gttaacagaa ccactattcg cgcatggacc aaacagggga tgccgtatct
    36121 taatgcggat cgcggaaagt ctggcggtta tcacatcggg catacattgc tttggtcttc
    36181 aggtaaaagc cgtcttgagg ccatcagata tcacgtagaa accagtgcgc tggaaaaaat
    36241 tatgtttgcc aggctgcttt catctgagcg tgacgagtac tccagtgaag aaacagaaca
    36301 tcgatttgat gaaggtttgc agatttacgg ctattcaccg gaagatgtga gcaaggcacg
    36361 aaataaaatg gctggctttc tggctgggtg gcgtcatgcc gtaagcgttc gccgtgccag
    36421 catggagcaa tcagccgata cagaacagta aagccgataa cttgttaatc actcgcagca
    36481 atgcatgtga tttgtgcaca ctttcggtta actggttcga tgtccggttt tagtgtctgt
    36541 tttttgcgca tgtccggttc atggaaagca tgtttttata tttttcatat ggttaacttg
    36601 tagagaaacc ggacatggat cccgaaaaat tttcataaat agtgaaaacg cgcgaggtcg
    36661 ccgccccgta acgggtccat aattccagga aggacccgac gacaccagac aatcagaacg
    36721 ataggtgcac aatgacagaa gccgaaatac tgggattaat ccgccgtgct ggtggaatca
    36781 gccagcagac tgacgaacag gccacgcagc cggacagcgt gacagccgaa aattatgcgc
    36841 gtgttgttgc tgaggtgatg cgccgtgatg gtatccagct taatgatgtg gatatgcgca
    36901 acatacggat ccgcgttctt gaaatgctgg cctacaatcg ccgcgtggca ctgtatcggg
    36961 aaacggagaa aattacatac cactggaaga agccggagcg gttgcggcgg taactggttg
    37021 atattttcga aattcgccag cggcgaacat cggggaaacc agcctaaccc gttgatattt
    37081 tcgggaacct caaattgagg aggtcggaaa cataacccgt catattctgg atgctgataa
    37141 ttaccacgca gcccattaag gcctaacccg ttgattcttt cggaaacagc cattggcgta
    37201 atccggcgcg gtaacttgct gaacttaaag caaagcgcaa aattgcgctg gctggtgggc
    37261 atgatgccga tatggtgatc cccataacgg aaatatccac cagcacggca acggcagaat
    37321 agccacaaca cagaaaaacc acgaatatgg ggtttttgtt atggcatggg catgatgacc
    37381 gcccctgctc tgaatggtgg aaatcaccac gcagatcatc caccagcttc gtttttataa
    37441 attaaacatc cgggaaaatc cgaccctgaa ttgtgcattt tttcagcaaa tcattttagt
    37501 tatacgtttg gttatacggt ttaaaagttg aatcatatac attattttaa atcatgaagt
    37561 tacacgctca attaaactac tctcgtgcaa aaatcccaag aaaaaaccaa cctttacggt
    37621 tggttttttt atatctgcaa ttaatttgat aaacagaccg tgacacatca cagcctgttt
    37681 attttctgtt atcagaacgt ccagaccact cccgcctgag tattccacgg cgattctacc
    37741 cctgcgccat tgccatagct gactgacaaa tgaccgctta acgtcggggt aaacgatgac
    37801 cttatcccag cctgataaac gccaggggtt ccagccacat cattattaaa tttgccatcg
    37861 tcattaactt tcacatggtt agaatcggcg tattcctgac gtacggcggc tttcagccag
    37921 ggttccagcg tcgtaccatt ttgcaggtcc atgtgatagc ttaccgccgt tcccgcttca
    37981 gcgcgtaata tccgggtatt tcccacatca gcgcgcatgc cgtttgataa cgtgtagtcc
    38041 tgaccatctg tggtaaagcc ggtaaaggcc agatagggtc taacactcca caatccgtca
    38101 acccaacgga acccgctctc aacatgagca cccgcgccgt tactattgta atcgccaaac
    38161 gctgttgccc cattactcat cttgccatgg atggtgttgg caaaacggtc aactttcacc
    38221 accccatcaa cataggcacc gttctgatgc tcccaaccgg cataagcccc cagggtatag
    38281 ctatcgatat taccttttcc gccgcgatca aaaccaatat cagaatgaga gtaaccaaag
    38341 aacaagccgc gaattgtact gctttcttca cgggagaaac ggctatcgat accgagcgtc
    38401 aggcccgtca atgtttgctc aaaaccagct cccgcatcag tggtcacgtt gttgcgggtg
    38461 ttaattgccg aactccacat cgccgtatcg taactaacgc tttttacgct accaagacgc
    38521 tcacgcacgg tgtccagttc tgcatcaaat accagcggtt gtgcggccgc catattcagc
    38581 acatcagtgg ttgaaggggt aatttgcgcg cgattctctg ccaaactcca gctatggttg
    38641 ccattatcca gcaaggtata ttcatacgta ccgatatcaa caacgcctcc ggcattgccc
    38701 aacgtaaatg cagcatcacc gccgcccgtt gttaccagtg taaggctatc tcctgctgcc
    38761 gggctggcac cggtgtccgt cacgaatatt ttgaaatcac ctgttgcctg accggtgacg
    38821 ttgagctgat cgctctgatg attagccata tcggtacgca tataaaaatt gccgtttccc
    38881 gacagggtat tggtggtcaa cgtaatgaaa ttttccgcac ttgccgtcac tgatgaacga
    38941 gtcactggct catccatcat aatcacattt gcgtcagaca tcgcaaggtt ggctaagttg
    39001 aggagggtgt tttggttcgt cgtagtgata tcggcaatga tggcccatac gctattttcg
    39061 agcgaaacgt ccgctttgct ggtatccacg gcaccatgcg ttctaaaaga agcgccctca
    39121 gaaaccacta ccttacccac caacatagcg ggtgcataat ttatttgcgg cttcatgacc
    39181 tcaagaatgc catcattccc tctgactgac acgttaacca ttgtgccagc ccagacatta
    39241 gcgcggccat tgttgataac catattttca gagatggctt tagaggaata cgtgatactt
    39301 ccattctggt ctgatcgacc gagatcatat accgcatttg cctgcacttt cgtaccagta
    39361 tcctttccca gcgattgcat agtggcatgc tcatcaagga tagtatcaat ggcctgcgtg
    39421 tcctccataa caatgagccc ggaaccatca tccagttcat aattttttga cacaccatct
    39481 tttatactaa attggccttt actgtttgta ccactcactt ccagcgcatt cgttgagaca
    39541 attaattttc cgcctgcttt tttatcaacg ccagttgcag tcccgccatc cataacctcc
    39601 agtaagccgc cactatctac agtagtatta taagcgaagt cattttcttc aactcgcaaa
    39661 cttccgccgt tttccaacag catattgtta gcaataccat tcttgatttc gaactgaccg
    39721 agacggtttg ttccgaatgc ctccatgacc cgcgtgcttg ctttaatagc accgcctgct
    39781 ttctgatcta ccgcaatcgc taatcccccc tctttaacct ccagtaatcc tcccttttcg
    39841 atagcggcaa ggacggtaat cccatctttt tcaacgatta agcgcccgcc tttaatcgtg
    39901 ggatcattcg ctgcgccata aacctgtagc acaccattat tgagggtagt ctggtttgcc
    39961 gcgccaccca tttgaacaat ttgataaccg tcatttaata ctgagtattc tgcggtacca
    40021 ttcatttcaa tgtactgata ccctccgtt
//
LOCUS       DAJTNN010000013        39779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTNN010000013 DAJTNN010000000
VERSION     DAJTNN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.013"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..2069)
                     /locus_tag="OO150_000646"
     CDS             complement(<1..2069)
                     /locus_tag="OO150_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5514893.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFITRYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVE"
     gene            complement(2166..2642)
                     /gene="greB"
                     /locus_tag="OO150_000647"
     CDS             complement(2166..2642)
                     /gene="greB"
                     /locus_tag="OO150_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5514894.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            2870..3589
                     /gene="ompR"
                     /locus_tag="OO150_000648"
     CDS             2870..3589
                     /gene="ompR"
                     /locus_tag="OO150_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5514895.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3586..4938
                     /gene="envZ"
                     /locus_tag="OO150_000649"
     CDS             3586..4938
                     /gene="envZ"
                     /locus_tag="OO150_000649"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5514896.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5014..6636)
                     /gene="pckA"
                     /locus_tag="OO150_000650"
     CDS             complement(5014..6636)
                     /gene="pckA"
                     /locus_tag="OO150_000650"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5514897.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7015..8739
                     /locus_tag="OO150_000651"
     CDS             7015..8739
                     /locus_tag="OO150_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5514898.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNCSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDVTFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(8874..9752)
                     /gene="hslO"
                     /locus_tag="OO150_000652"
     CDS             complement(8874..9752)
                     /gene="hslO"
                     /locus_tag="OO150_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5514899.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(9777..10178)
                     /gene="hslR"
                     /locus_tag="OO150_000653"
     CDS             complement(9777..10178)
                     /gene="hslR"
                     /locus_tag="OO150_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5514900.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10189..10857)
                     /gene="yrfG"
                     /locus_tag="OO150_000654"
     CDS             complement(10189..10857)
                     /gene="yrfG"
                     /locus_tag="OO150_000654"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5514901.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(10922..13057)
                     /gene="igaA"
                     /locus_tag="OO150_000655"
     CDS             complement(10922..13057)
                     /gene="igaA"
                     /locus_tag="OO150_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5514902.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRSQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDCHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13377..13937
                     /gene="nudE"
                     /locus_tag="OO150_000656"
     CDS             13377..13937
                     /gene="nudE"
                     /locus_tag="OO150_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5514903.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14102..16654)
                     /gene="mrcA"
                     /locus_tag="OO150_000657"
     CDS             complement(14102..16654)
                     /gene="mrcA"
                     /locus_tag="OO150_000657"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5514904.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            16774..17553
                     /gene="hofM"
                     /locus_tag="OO150_000658"
     CDS             16774..17553
                     /gene="hofM"
                     /locus_tag="OO150_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5514905.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALFVDPDS
                     VAICGEGGFDPWEAVSVRQPLLPPPGGDFAIALGLALGKAY"
     gene            17553..18092
                     /gene="hofN"
                     /locus_tag="OO150_000659"
     CDS             17553..18092
                     /gene="hofN"
                     /locus_tag="OO150_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5514906.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRKQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18076..18516
                     /gene="hofO"
                     /locus_tag="OO150_000660"
     CDS             18076..18516
                     /gene="hofO"
                     /locus_tag="OO150_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5514907.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18506..18910
                     /gene="hofP"
                     /locus_tag="OO150_000661"
     CDS             18506..18910
                     /gene="hofP"
                     /locus_tag="OO150_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR5514908.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            18822..20060
                     /gene="hofQ"
                     /locus_tag="OO150_000662"
     CDS             18822..20060
                     /gene="hofQ"
                     /locus_tag="OO150_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5514909.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGIEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            20461..20982
                     /gene="aroK"
                     /locus_tag="OO150_000663"
     CDS             20461..20982
                     /gene="aroK"
                     /locus_tag="OO150_000663"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5514910.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21039..22127
                     /gene="aroB"
                     /locus_tag="OO150_000664"
     CDS             21039..22127
                     /gene="aroB"
                     /locus_tag="OO150_000664"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5514911.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22219..23505
                     /gene="damX"
                     /locus_tag="OO150_000665"
     CDS             22219..23505
                     /gene="damX"
                     /locus_tag="OO150_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5514912.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGDKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23612..24448
                     /gene="dam"
                     /locus_tag="OO150_000666"
     CDS             23612..24448
                     /gene="dam"
                     /locus_tag="OO150_000666"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5514913.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24466..25143
                     /gene="rpe"
                     /locus_tag="OO150_000667"
     CDS             24466..25143
                     /gene="rpe"
                     /locus_tag="OO150_000667"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5514914.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25136..25894
                     /gene="gph"
                     /locus_tag="OO150_000668"
     CDS             25136..25894
                     /gene="gph"
                     /locus_tag="OO150_000668"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5514915.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            25887..26891
                     /gene="trpS"
                     /locus_tag="OO150_000669"
     CDS             25887..26891
                     /gene="trpS"
                     /locus_tag="OO150_000669"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5514916.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            27160..28065
                     /locus_tag="OO150_000670"
     CDS             27160..28065
                     /locus_tag="OO150_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514917.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            28082..28444
                     /locus_tag="OO150_000671"
     CDS             28082..28444
                     /locus_tag="OO150_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5514918.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            28528..29691
                     /locus_tag="OO150_000672"
     CDS             28528..29691
                     /locus_tag="OO150_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5514919.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            29691..30917
                     /locus_tag="OO150_000673"
     CDS             29691..30917
                     /locus_tag="OO150_000673"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5514920.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            30914..31792
                     /locus_tag="OO150_000674"
     CDS             30914..31792
                     /locus_tag="OO150_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5514921.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            31803..32156
                     /locus_tag="OO150_000675"
     CDS             31803..32156
                     /locus_tag="OO150_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5514922.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            32168..33472
                     /locus_tag="OO150_000676"
     CDS             32168..33472
                     /locus_tag="OO150_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5514923.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            33484..34569
                     /locus_tag="OO150_000677"
     CDS             33484..34569
                     /locus_tag="OO150_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5514924.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGEVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34721..35452)
                     /gene="phoB"
                     /locus_tag="OO150_000678"
     CDS             complement(34721..35452)
                     /gene="phoB"
                     /locus_tag="OO150_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5514925.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCKKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(35552..36337)
                     /gene="frlD"
                     /locus_tag="OO150_000679"
     CDS             complement(35552..36337)
                     /gene="frlD"
                     /locus_tag="OO150_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5514926.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(36334..37164)
                     /gene="frlC"
                     /locus_tag="OO150_000680"
     CDS             complement(36334..37164)
                     /gene="frlC"
                     /locus_tag="OO150_000680"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5514927.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNIVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(37214..38236)
                     /gene="frlB"
                     /locus_tag="OO150_000681"
     CDS             complement(37214..38236)
                     /gene="frlB"
                     /locus_tag="OO150_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5514928.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQAAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(38257..39645)
                     /gene="frlA"
                     /locus_tag="OO150_000682"
     CDS             complement(38257..39645)
                     /gene="frlA"
                     /locus_tag="OO150_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR5514929.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLTFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISDLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGLFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
BASE COUNT         9198 a        10091 c        10944 g         9546 t
ORIGIN      
        1 tcttccacaa acttgttcga caatgaagag atgtgaacca ggccgtcctg atgcacgccg
       61 atatcgacaa acgcgccaaa gttggtgacg ttggtcaccg caccttcgag gatcataccc
      121 ggttgcaggt cgttcattgt ctcgacgcca tcggcaaact gagcggtttt aaattccgga
      181 cgcggatcgc gacccggttt ttccagctct ttgatgatgt cagttactgt cggcacaccg
      241 aatttttcat cagtaaagtc agacgctttc aggttacgca gttcgctgct gttacccatc
      301 agatctttca gtgcctgctg tgttgctgcc agaatgcgtt ccaccaccgg ataggcttcc
      361 gggtggacgg tagaagcatc cagcgggtta tcaccgtggt taatgcgcaa gaagcccgcg
      421 cactgctcga aggctttcgg ccccagacgg ctgactttca gcagttgctg acggttctgg
      481 aactggccgt tctcatcgcg ccaggccacg atgttttgcg ccatcatgcg cgtcaggccc
      541 gccacgcggg ttaacagcgg aacagaagca gtgttgagat cgacgccaac ggcgtttacg
      601 cagtcttcta ctactgcgtc cagtttgcgg gccagttgcg tctggctgac gtcatgctga
      661 tactgaccta cgccgataga tttcggatcg attttcacca gctccgccag cggatcctgc
      721 aaacggcggg cgatagacac cgcgccacgc agcgaaacgt cgagatccgg gaactcctgc
      781 gctgccagct cggaagctga gtaaaccgac gcgccagctt cgctgacgat cactttctgt
      841 gcggtcactt tcgggaactg cttctgcacg tcgagataga aacgttcagt ttcgcgggaa
      901 gctgtaccgt tgccgatcgc caccagttca acgttatgtt tttcacacaa ggcagcaacg
      961 gtcatcgctg cttttgcggc ttgtccggtg tgcgggtaaa tggtatcggt cgctaccagt
     1021 ttgccagtgg catcgaccac cgccactttt accccagtac gcagacccgg atcgaggccc
     1081 atcgttgcac gcagtccggc aggggccgcc atcagcagat cgtgcaggtt acgggcaaag
     1141 acgttgattg cttcatcttc cgcgcgttcg cgcacggtac ccatcagttc ggtttccaga
     1201 tgcatcagca ccttgatgcg ccaggtccag ctcactacgc ctttgcgcca gctatccgcc
     1261 ggggcattgt tcaggcgcag gccaaggtga tccatgatga tttgctcgca atagctctct
     1321 ttgggcggct catcgaactg cggatcggca ttcagcgaaa gctggagtac gccttcgtta
     1381 cgcccacgga acatcgccag agcgcggtga gaaggcaccg tggacaacgg ttcgtgatga
     1441 tcgaaatagt cgcggaattt cgccccttcc tcttctttac cgctcaccac cgtagaaacc
     1501 aaatgcgcgt tcttccacag ataatcacgc actttcgcca gcagcgcggc atcttcggca
     1561 aaccgttcca tcaggatata gcgcgcgcca tccagcgcgg ctttggtatc tgccacgcct
     1621 ttatcggcat caacatattg tgcagcggcg acttctggcg tgtgtgacgg atcgctccac
     1681 agcaggtcag ccaacggctc aagccctgct tcaatggcga tttgcccgcg ggtgcggcgt
     1741 ttaggtttgt agggcaggta gaggtcttcg agttcggttt tgcttagggt ggcgttgatg
     1801 gccttcgcca gatcatcggt gagtttgcct tgctcggaaa tggacttgag gatcgcctga
     1861 cgtctctctt ccagctcgcg cagatagctc agacgcgttt ccagattacg cagctgcgtg
     1921 tcatccagac cgccggtgat ttccttacga taacgtgtga taaacggcac ggtattccct
     1981 tcgtcaagca ggcgaacggc agcgtcaacc tgttccgggc gcgcctgaat ttcacccgca
     2041 ataatgcggc agaacgaatc attcatcatg gtttgagttc atcttttcgg atcaaaaatc
     2101 aggggatagt tatacggact ggctggcaaa aatgccagcc atcggcagga ggttaagact
     2161 cttccttacg gtttcacgta ctcgatagca ttaacatacc agctcgcttc cccggcaggg
     2221 gtattcacca ccgccagatc gccgacttct tttttcagca atgcgcgggc catcggggaa
     2281 tcgatagaga tgtaatcttt acggccaaaa atttcatcgt agccgacaat acggaaacgg
     2341 tgagtcacgc catcgtcgtt ttcaatctcc acccacgcgc caaaaaagac tttgccttcc
     2401 tgctgagggg agtaatcgac gattttgaga ttttccatgc atttagtgag atagcgcacg
     2461 cgacggtcga tttcacgcag acgcttttta ttatactgat agtcagcatt ttcgctgcgg
     2521 tcgcccagac ttgcggccca ggtcaccttt tttgtgacct ccgggcgttc ttcacgccag
     2581 agataattaa gctcttgttt gagtttttca tacccttccc gggtaaccag gggcgttttc
     2641 atctcgttga ttccctttgt ctgtttgata atgcgcacat tgggtataac gtgatcatat
     2701 caacagaatc aataatgttt cgccgaataa attgtatact taagctgctg tttaatatgc
     2761 tttgtaacaa tttaggctga aattcatacc agatttagct ggtgacgaac gtgagctttt
     2821 ttaagaatac acgcttacaa attgttgcga acctttggga gtacaaacaa tgcaagagaa
     2881 ctacaagatt ctggtggtcg atgacgacat gcgcctgcgt gcgctgctgg aacgttatct
     2941 caccgaacaa ggcttccagg ttcgaagcgt cgctaatgca gaacagatgg atcgcctgct
     3001 gactcgtgaa tctttccatc ttatggtact ggatttaatg ttacctggtg aagatggctt
     3061 gtcgatttgc cgacgtcttc gtagtcagag caacccgatg ccgatcatta tggtgacggc
     3121 gaaaggggaa gaagtggacc gtatcgtagg cctggagatt ggcgctgacg actacattcc
     3181 aaaaccgttt aacccgcgtg aactgctggc ccgtattcgt gcggtgctgc gtcgtcaggc
     3241 gaacgaactg ccaggcgcac cgtcacagga agaggcggta attgctttcg gtaagttcaa
     3301 acttaacctc ggtacgcgcg aaatgttccg cgaagatgag ccgatgccgc tcaccagcgg
     3361 tgagtttgcg gtactgaagg cactggtcag ccatccgcgt gagccgctct cccgcgataa
     3421 gctgatgaac cttgcccgtg gtcgtgaata ttccgcaatg gaacgctcca tcgacgtgca
     3481 gatttcgcgt ctgcgccgca tggtagaaga agatccagcg catccgcgtt acattcagac
     3541 cgtttggggc ctgggctacg tctttgtacc ggacggctct aaagcatgag gcgattgcgc
     3601 ttctcgccac gaagttcatt tgcccgtacg ttattgctca tcgtcacctt gctgttcgcc
     3661 agcctggtga cgacttatct ggtggtgctg aacttcgcga ttttgccgag cctccagcag
     3721 tttaataaag tcctcgcgta cgaagtgcgt atgttgatga ccgacaaact gcaactggag
     3781 gacggcacgc agttggttgt gcctcccgct ttccgtcggg agatctaccg tgagctgggg
     3841 atctctctct actccaacga ggctgccgaa gaggcaggtc tgcgttgggc gcaacactat
     3901 gaattcttaa gccatcagat ggcacagcaa ctgggcggcc cgacggaagt gcgcgttgag
     3961 gtcaacaaaa gttcgcctgt cgtctggctg aaaacctggc tgtcgcccaa tatctgggta
     4021 cgcgtgccgc tgaccgaaat tcatcagggc gatttctctc cgctgttccg ctatacgctg
     4081 gcgattatgc tattggcgat aggcggggcg tggctgttta ttcgtatcca gaaccgaccg
     4141 ttggtcgatc tcgaacatgc tgctttgcag gtcggtaaag ggattattcc accgccgctg
     4201 cgtgaatatg gcgcgtccga ggtacgttcg gttacccgcg cctttaacca tatggcggct
     4261 ggtgttaagc aactggcgga tgaccgcacg ctgctgatgg cgggggtaag tcatgactta
     4321 cgcacgccgc tgacgcgtat tcgcctggcg accgagatga tgagcgaaca ggatggctac
     4381 ctggccgaat cgatcaataa agatatcgaa gagtgcaacg ccatcattga gcagtttatc
     4441 gactacctgc gcaccgggca ggagatgccg atggaaatgg cggatctcaa cgcagtactc
     4501 ggtgaggtta ttgctgccga aagtggctat gagcgggaaa ttgaaaccgc gctttacccc
     4561 ggcagcattg aagtgaaaat gcacccgctg tcgatcaaac gcgcggtggc gaatatggtg
     4621 gtcaacgccg cccgttacgg caatggctgg atcaaagtca gcagcgggac ggagccgaat
     4681 cgcgcctggt tccaggtgga agatgacggt ccgggaattg cgccggaaca acgtaagcac
     4741 ctgttccagc cgtttgtccg cggcgacagt gcacgcacca ttagcggcac gggattaggg
     4801 ctggcaattg tgcagcgtat cgtggataac cataacggga tgctggagct tggcaccagc
     4861 gagcggggcg ggctttccat tcgcgcctgg ctgccagtgc cggtaacgcg ggcgcagggc
     4921 acgacaaaag aagggtaaat aaacgggagg cgaaggtgcc tcccgttttg ctttctataa
     4981 gatactggat agatattctc cagcttcaaa tcattacagt ttcggaccag ccgctaccag
     5041 cgcggcaccc gcaggggtgt cggtgtattt atcgaagttg tcgataaaca gtttcgccag
     5101 ggtttcggct ttttcctgcc actgttccgg agaagcgtag gtgttacgcg gatcgagaat
     5161 cttcgtgtct acgcccggca gttcggttgg gatcgccagg ttaaacatcg gcagagtgaa
     5221 ggtttctgca ttatccagcg aaccgttgag gatggcgtcg ataatggcgc gggtatcttt
     5281 aatcgagata cgtttgcctg tgccgttcca gccagtgtta accagataag cctgcgcgcc
     5341 cgccgcctgc atacgtttca ccagcacttc tgcgtactga gtcgggtgca gcgacaggaa
     5401 tgccgcgccg aagcaagcgg agaaggttgg cgtcggttcg gtgatgccac gctcagtacc
     5461 ggccagtttg gcggtgaagc cagagaggaa gtgatactgg gtttgatcgg cagtcaggcg
     5521 agaaaccggc ggcaacacgc cgaaagcatc agcagtcagg aagataacct tagtcgcgtg
     5581 gcccgctttg gaaaccggct taacgatgtt atcgatgtga tagattggat aagaaacgcg
     5641 ggtgttctcg gtttttgaac catcatcaaa gtcgatagtg ccatcttcac gcacggtgac
     5701 gttttccagc agcgcatcac gacggatagc gttgtagatt tcaggttccg cttctttcga
     5761 cagcttgata gtttttgcgt agcagccgcc ttcgaaatta aacacgccgt catcgtccca
     5821 gccgtgttcg tcatcgccaa tcaggcggcg tttcgggtcg gtggaaaggg tggttttacc
     5881 ggtgccggaa aggccgaaga acaccgcaac atcgcctttc tcgccaacgt tggcggagca
     5941 gtgcatagaa gcgatacctt tcagcggcag caggtagttc atcatcgaga acatcccttt
     6001 cttcatttcg ccgccgtacc aggtgccgcc aatcagctgc atgcgctcgg tcaggttaaa
     6061 cgccacgaag ttttcggagt tcagaccctg ttctttccac tgcgggttag tgcacttcgc
     6121 gccgttcata acgataaagt ctggtttgaa acctgccagc tcttcatcgc tcgggcgaat
     6181 aaacatgttt ttgacaaaat gcgcctgcca ggccacttcg gtgatgaaac ggacggaaag
     6241 acgagtatcc gggttcgcac cacagaaagc gtcgacaacg aacagacgtt tgccggaaag
     6301 ctgcctggtc accaggcctt tcagatgctg ccaggtttcc ggagagagag gtttgttgtc
     6361 gttcttacct ttgcctttgt ctgcccacca gaaagtatcg cgagtggtat cgtcacggac
     6421 gatatactta tcttttggtg aacgaccggt gaagatcccg gtatcgacgg caacggcacc
     6481 cagattagtt aacaccccgc gctcataacc tgtcaggctc ggatcgagct cttcctgata
     6541 cagcaggtcg tagcttgggt tgtaaacgat atcatgtacg tcactgatac cataagcctc
     6601 gagttcttgc ggggtcaaac cattgttaac gcgcatttca ctgctcctta gccaatatgt
     6661 attgcctgaa tagtaaagtc tttttggggg tgttaaccgc gacaaggctc atagatttac
     6721 gtatctggag aaattcatat ttgacaaaaa accccgtgat tcctgttacg aaacggttgc
     6781 tattatcgca gaaatagcat tttaggtgtg gaaaatgttc tcaaaaggtt aaatcttggt
     6841 ttatatggcg ggaatattgt gagtggaatc gcattcctta atgaaattga tgcaaattca
     6901 atcggtaaag tatcgattca ccacgctaac tttttttgat tattttctga aaaaagcaaa
     6961 atactactgc ccggatgcga taattaaccc accttttgat tctggataac caacatggat
     7021 aacgttgaac tttcaccggc aacacgctgg gggatgattg ctaccggatt acttcagggg
     7081 ctggtttgct acctgttgat cgcctggctt gccggaaaaa atcacagttg gattgtttat
     7141 ggcgtgcctg cgaccgttgc gttttcatcc gttttactct tctccgtgat ctcttttaaa
     7201 cagaagcgcc tttggggatg gctggcgctg gtgtttattg ccacgacagg gatgagcggt
     7261 tggctgaagt ggcaaactga tggcatgaac ccctggagag ctgaaaaggc gatttgggat
     7321 tttggctgct atctgctgtt gatggcaatg ttgttgctac cgtggataca acaaagcctt
     7381 cgtatccgca attgcagtag ccgctatagc tacttttacc agtcagtatg gcataacgta
     7441 cttatattac tggtgatttt tctcgccaat ggcctgacgt ggctggtgct tttactgtgg
     7501 ggtgagttgt ttaaacttgt tggcatcaag tttttcaata cgctcttttt tgcaaccgac
     7561 tggtttatgt atcttacatt aggtctggtt accgcactgg cggtgattct cgcgcgcaca
     7621 cagtcacgtt taatcgactc tattcaaaag ttgttcacgt taatcgccac ggggttgctg
     7681 ccgttagtat cattgctaac cctgatgttt atcatcactc tgccgtttac gggcctgagc
     7741 gcgatttctc gccacatctc cgccgccggg ttgctgttaa cgctggcctt tttgcaattg
     7801 atcttaatgg ctattgtccg cgatccgcaa aaagcgtcac ttccctggac ggggccgttg
     7861 cgttgcctga ttaaaaccgc tttgctggtt gctccgctgt atgtgttcgt cgccgcctgg
     7921 gcgttatggc tgcgggtcgc tcagtacggc tggactgtcg accgcttgca gggcgcgctg
     7981 gcggtgctgg tgttactggt gtggtcgctg gggtattttg tcagtatcgt ctggcgtaat
     8041 gggcaaaatc cccttgttct tcagggcaaa gtgaaccttg cggtttcgtt attggtgttg
     8101 gtgatactgg tgcttcttaa ttcgccggtg ctggacagta tgcgtattag cgtgaacagt
     8161 catatggcgc gttatcagag cggcaaaaac acgccagacc aggtaactat ctacatgctc
     8221 gaacagagcg gtcgctatgg acgtgcggcg cttgagtcgc tgaaaagcga tgccgggttt
     8281 atgaaagacc cgaaacgcgc gcgggatctg ctgatggcgt tagatggaga gcaacatctt
     8341 caggaacagg tatcggaaaa agtattagcc gaaaatgtgt taattgcccc tggttctgtt
     8401 aaaccggatg tgacattctg gtcggcctta atccaggatc gctataacgt gatgacctgt
     8461 attgaaaaag acgcctgcgt cctggtcgag caagatctga atagtgatgg tcaggcggag
     8521 cggatcctgt ttgcttttaa tgatgacaga gtcattgtct atggctttga ctcagacaga
     8581 aaagaatggg acgcgcttga tatgagttta cttccgaacg aaataacgaa agaaaaatta
     8641 ctcacagctg cgaaggatgg aaaactgggg acgaggccga aagcgtggcg cgatctgacg
     8701 gtagatggtg aaacgctgga aataaactta agtaagtgac aacgctttta tcggatacga
     8761 cgctgtatct gaccaaaata atttgcgatt tatttcatag gcgctggtaa gcgcagcaca
     8821 tcaggccatt aagttgtaaa atccggcaga gccctctctg ccggacatac tcattaatga
     8881 acttgcggat ctgccggaga cgcgttgttg cggatttcag caatatccat cgcattgaac
     8941 agatagtggt taccgcagta atcacaatgc atgtcaattt cgccatcttc cgccaggatg
     9001 ctatcaactt cttcatcagg cagcgttttc agcgcatcgg cgcaatgttc acgcgagcag
     9061 gtgcatttga actccacatc ctgcggatcg taaaccgtca cctcttcttc gtgatacaaa
     9121 cgccacaaca cttcgtttgc cggtaaggtc agcagttctt cggttttgat ggtttcggtt
     9181 agcgtcgcca ggtggtcaaa gtcgtcctgc tgggcatttt gcgcaggcat tacctgcaac
     9241 aacataccgc ctgcagccgg tttgccgtct acgtcgccgg tgcgaataaa caggcgcgtc
     9301 ggcagctgtt cagaacgcat aaagtaatct tccaggcagg ccgccagggt atcaccttcc
     9361 agaccaacta cgccctgata gcgttcgcct tcgctcgggg taatggtgat caccacgtaa
     9421 ccattgccga ccagcgtttt caggtcggca ttttctggaa tttcgccctg cacgcgcgcc
     9481 acaccgcgca tttgctggtt attgttaccg ttaataaccg ccagattcat cggaccgtcg
     9541 ccctgcagct gtacggtgat atcaccatca aacttcagcg tagcggttaa caggctggtc
     9601 gcaaccagca gttctgccag cacgttttta acgggctgcg gataatcgtg gttctcaagg
     9661 atctgttgca gggtttccga aacggttacc agttcgccgc gaacggcaaa attttcaaac
     9721 agatagcgat gtaattggtc atgttgcggc ataatcatct ctcttgcagg tgacagttat
     9781 tcactgtcgc cgtgtttaaa tcgtaacagg tcgcggcgct cttttttgtc cgggcgtcgg
     9841 tccgggtgcg gcatggttaa ggcattaagt ttacgtgcca tcgccatttt ttcgcgtttc
     9901 tctacacttt ccgcagtctc ttcatacagc aaggctgcct cgctggcggg gcgacgctgt
     9961 tcagtaatcg cctttacaat caccgtgcgt tcgtcatttc cctggcgcag agtgagcgtg
    10021 gcattcagct cgacgatttt gctcggcttg ctgcgctgcc cgttgtaatg caccttaccg
    10081 ccttcaatca tttcacgggc tagcgcgcgg gttttataaa aacgggcagc ccatagccat
    10141 ttatccagtc gaacctcaac agcaggtttc tctttcatgg cgtctccttc acattagcga
    10201 ggggatcagg cggcggtagt cattcagtga cggatggcgt tgatactgtt tctcggcaat
    10261 cccggaatca ggattagtca cgccgaggca gtaacgaata ccaaattgcg cggcagcatc
    10321 gagaatcgct tcgctgtcat caataaacag cgttctttca gctttcagac ccgtagcttc
    10381 ggccaccgca tgccataacc gctgatcctc tttcggataa ccaaatgtgt gggtggaaag
    10441 taataaatca aggtgtgcgt ccagacccgt atgctcaagt tttaccgcca ggttgtgcgg
    10501 atgagcattg gtgagcaaaa ttcgctgctt accgctggct ttcagtgcct caagaaacgg
    10561 aatggtatct tcacgcagta cggcgcgcgg tcccatctcg gtggtcattg cacagatatc
    10621 cagacccagt tgctcactcc agtaatcaag acagtaccag tttagcgtat gctgtacgtc
    10681 gtgatattgc tggcgcatat attccatcgc ttcctgtggc gtaaccccgt ttttcgcgcc
    10741 ccatgtttca ggcaccagct tttgccagaa atagttatcg aaggcgaggt cgagcaacgt
    10801 gccgtccata tccagcagaa cggtatctac gtcctgccag gcaatgttga tatgcatgag
    10861 ggaaatctcc agagtgaagc aatttgcgcg acagggtagc ataacctgtc gcgcaaacgt
    10921 gttattcgat aaggctttct gaaggggtga tcagttgcgg gttcaggcag ctttcataat
    10981 aggcctgaat ctccatcatg cgagtgcgat gacgctggta acgacgccag gcctgtacgc
    11041 cattgtaaat ggcgctaccc agcatcgtca gtagcagcaa tgtggtgctg agatagcgcc
    11101 acaggccgga acgatccggg atcggatgaa ggccaatatg ctgcgtacca ttggcgtcag
    11161 tgaagatttt tgtgacgata ccttcggcgt taaacggcgt atgcatcagc atttgtgcca
    11221 gtttctggaa agcgttccac tgttcttgcg gcgggtagtc gtaaagtgat gccgaaggcc
    11281 agggctgatc aacaaaatcg ctgccttcgt cgctgacaat caggaatccg ccgggggccg
    11341 gactgtttag tgattgtgcc gctcgcgccg tttcatgcgt gataaacggc gcggtggagg
    11401 ttgccaccag gttatccagc gattccgcac tcaccgggcg taataacaca ttcacgccat
    11461 cgagcttccc ggcgttggcg cgttttacca gcgcgtccca gtctttactg ttgccgagat
    11521 tgaccagcgc atttttcagt cgcacacagt catctttggc agaacataaa tccgctgtct
    11581 tcagtacaat gtcgccaaaa tcatcaagca ataccatgcc ggatttttga atcgctgaac
    11641 gtaatgaggc actgacgcga gattcatctt ccggtttagg gtgcagctgg cgattaactg
    11701 cttcagtcaa tgccgtcgct ttgttgacca gttcagattc tggtaatggt aatgagcggg
    11761 cgtcattcca gatgatctgc gagcagtcaa acggtaaaaa aggtgaattg gttttcgcgc
    11821 tccaggttcc ggaagtgcga atattacaca ttcccgtacc gctaatacgc aatgtatcgc
    11881 ctacccgcac gccagcgtca gccagttgtt ttacgctggt ggcttcaatg gtctgcgcgc
    11941 ctttcatcca tgagagagtg aatttcagcg gcatatccag cgggatccag aataacagca
    12001 taaacagcac cagcagcgag cccgcagcga taatcgtact gcgcagccag tgctgtaacg
    12061 gaaagttttt tacttcatca tgcagcgaaa gatatcgccc ctgacgcact acgtggcagt
    12121 cgagatagat atcaatatcg gtttgttgat cgagatcctg agcaatgtat ggctgccagt
    12181 gtgcgggata gaccaggtcg ataataccga gtgaaatatt gttgatctgt tcctgatcgt
    12241 tttcgccaaa caatccccaa cgacggggtg taccgcgcag acaatgaatt tcccgcaggg
    12301 aggattttgc cgggggcgcg aatagccccc acagacctgc gcccagcagc agtaatgcgc
    12361 cgcctgccag ccacggaaca aatacatccg gggtaatcag gcagaaaaag aacatcagga
    12421 aggaggcgac gatcagcaac gcttcacgca gcccgcgcgg acgactcagc gcatattctt
    12481 catgcgtttc tttgcgaata ttgagtagct cgatttgctc actctcttcc ccgcgaatcg
    12541 acgcctgcgt ggacggaaca ggttgcaagg catagccgcg cgtttcctgc atgtactcct
    12601 gtagcgtatg accgttgagt gaaataacca acggcagcga atcggtatga atcagttcaa
    12661 cggtattctc atcgttgatg tactgttccc agaagggggg caggtggact tctaccgaat
    12721 cgaggtagta acgccattta ttcggatcgt cggtagagat gccgtaacgt gtgatagcgt
    12781 gtgtcagcat catgacgttg ttgctttcgg cattcagcgc cagagagatc ggtgccgcgc
    12841 tagctcccgt tgggccaggc acctgtaata cctgcgtcag gctctcaaga taattttcaa
    12901 cggcgctacg ttcttcaggt gtgagtttac gcgtttgcgc atccgcgaag gcgttggtcc
    12961 agggcagctg actccgtctg gatcgcactt ttatcagcca tcccgcaagt agtgagcagg
    13021 ccagcaaagc agctaaaaaa atcacaatgg tgctcatgct ttccccatct tacttagtct
    13081 gtcaggcgtg gtcagtcgca ccagacacaa tacatgattc tgcggttggc tatcggcaag
    13141 cgtggagttt aacttgagca tcattaagca catcacagta accgctgatc atagcaaacc
    13201 ctctgaaggc ggaatatcag caaattccta gagttatttc aacctattga cttttaattt
    13261 gatttgagga cgattattta ttaagttaca taaatgtaaa taacatgcaa ttcacattgc
    13321 cgaaagcgtg tccgatatcg cacaataacc gctgatttca cagcatagat cccacgatga
    13381 gcaaatcatt acaaaaaccc accattctga atgttgaaac tgtagcccgt tcccgactgt
    13441 ttaccgtcga gagcgtggat ctggagttca gcaatggcgt gcggcgtgtt tatgaacgaa
    13501 tgcgtccaac caaccgggaa gcagtgatga ttgtgccgat tgtggacgat cacctgatcc
    13561 tgatccgcga atacgcagtg ggaactgaat cctacgaatt aggtttttcg aaaggattaa
    13621 ttgatccggg tgaaagcgtc tacgaagccg ctaaccgcga gctaaaagaa gaggttggat
    13681 ttggtgcgaa cgatctgact tttttgaaga agctcagcat ggcaccgtct tacttttcca
    13741 gcaaaatgaa tatcgtggtc gcgcaagatc tctacccgga atcgctggaa ggcgatgagc
    13801 cagagccgct accacaggtg cgctggccgc tggcgcatat gatggatttg ctggaagacc
    13861 ctgacttcaa cgaagcgcgt aatgtcagcg cgctgttcct cgtgcgcgaa tggttgaaag
    13921 ggcaggggcg agtgtaaatg cgtgaagttt gcctgatgca caacgcttat caggcttatg
    13981 gggttcctgc aatatattga tttttcacga ttttgttagc cgggtaacgc ggtcacccgc
    14041 atccagcata aacaaatgca ctttgtcagc caactgaaaa ggcgccgaag cgccttttta
    14101 atcagaacaa ttcctgtgcc tcaccattat cgataatggt cgttcccacc tcgtgcaccg
    14161 cctgttgtgt cggctgcgta ccttcgatga aatactcttc gcggctgttg ccaccattag
    14221 ctaactgccc ggtgctgcga tcgatattca ccgtcacaat acccggtggc ggcgtcagcg
    14281 gctgctccgg cacaccttca agaacggctt tcatataagc gtcccatgca ggctgggcac
    14341 tcttggctcc gccttcgtaa cctgagatct gatctttaat cgctccggaa gccgttgtat
    14401 gaccgagatt acgacggtga tcatcaaagc caatccagac cgaggtcaca acgcccggac
    14461 cgtaacccga gaaccacgca tctttcgaac tgttagtggt accggttttc ccgccgatat
    14521 cgcgacgctg caaatcacga cctgcacgcc agccagtacc ctgccagcct ggctcaccaa
    14581 agatattggt gttcaaagca ctcttaatca ggaatgccag cggagtgttg atgacgtgcg
    14641 gtgcgtactc ctgcgcgcca gtcttcgcca ctaacgcctg atttgcctgc tccagctgcg
    14701 gcattggtac agaaacattc tgctgctcgc gggagatagc gacatcttca acatcgttat
    14761 tttccagcac gttcgatttc tgcgtatcac cgtaaatcac cggaatatcg cattccgggc
    14821 aggctacttt cggtttcgct tcgaaaatca cgccgccctg atcgttttca attttgctga
    14881 taaaccacgg gtccaccagg aagccgccgt tcgccatgac cgcgtagccg cgcgccacct
    14941 gcattggggt gaaggacgct gaacccagcg ccagcgattc ggtgtggaca atgttttgcg
    15001 ccgggaagcc gaagcgttgc agatattctg cagcgtagtc gacgcccata gcccgcattg
    15061 cacgtaccat caccacgttt ttcgactgac ccagcccctg acgtaagcga attggaccag
    15121 catattgtgg cggcgagttt ttcggctgcc agtcagaacc ggcacctgcg tcccagcgag
    15181 aaattggcac atcgttcaac atacttgcca gcgtcagacc tttatccatc gccgcggtgt
    15241 agaggaacgg tttgatgttg gaacccacct gacgcagtgc ctgggtggcg cggttaaact
    15301 tgctctgatt gaaatcaaag ccaccgacca gcgccataac ggcaccgttt tgcggattga
    15361 tcgacaccag cgccgagttc acttccggca cttgtgccag ccaccatgca tcgccaacct
    15421 gacgaaccca gatttgctga cccgtttgca gaacatcggt cactttacgc ggcgtcggtc
    15481 cctgctgagt atccgaacgg taaggacgcg cccagcgaac gccttccata ctcaatgcga
    15541 cggtcgaccc gtccgccagc atcgccgtcg ctccctgagg attggcgctg gtgactgcgg
    15601 caggcagcag cggaccatag gtcggcagcg ccttcagcgt atcggtaatc ttgttgttat
    15661 cccacgccga ctcgcccact ttccacagca catttgccgg gccgcgatag ccgtggcgca
    15721 tgtcgtagtc cagcacgtta ttacgtaccg cctgctgtgc ggcctgctgc actttgcggg
    15781 tgatggtggt gtaaatgcga taaccgtctt cataggcact ttcgccataa cggttataca
    15841 tctcctggcg caccatttcg ctcaggtacg gcgcagagaa agcaatctcc ggcgcgtgat
    15901 agttagcgtt aatcgcctca gtgcgtgtct gatcgaactg ttgttgggtg atataccctt
    15961 catccagcat ccgcgacagt acgacgttac gccgcgcgac ggcacgatcc attgagtaga
    16021 gcggattaaa ggtggaaggc gctttcggca gcccggctat cacagccatt tcgttcagcg
    16081 tcagttggtc gaccgtttta ccgaaataga cttgtgccgc agcaccgaca ccataggcgc
    16141 ggtaaccgag gtaaatctta ttcagataaa gctcgaggat ctcgtctttc gtcagcaact
    16201 gttcaatgcg aatcgcgagg aagacttcct taatcttacg catcagcgtg cgttctggac
    16261 tgaggaagaa gtttctcgcc agctgctggg taatggtact cgccccttgt gacgcgtggc
    16321 cggagaacag cgccacactt gccgcacgga agatccccac cgggtcaacg ccgtgatgct
    16381 cgtagaagcg gctgtcttcc gtcgcgataa aggctttcac catctccggc gggatttgat
    16441 ccaacgtaac cggaatacga cgtttctcac cgtattgagc aatcagctcg ccatcggcgc
    16501 tgtaaatctg catcggaatt tgcaggcgaa catcttttaa tgtcgccaca tccggcagtt
    16561 gtggctcgat gtagcggtat aggccataaa tcgagcctgc tcccagcaga atgcaacaga
    16621 ctgcaaggat caaaaaatac tttacgaact tcactggaaa tttcccattt agtttcattt
    16681 gggcagttta taaacaaacg cgcgttagta taaaggcaag ccagacgcat tgatataccc
    16741 gtcagagtga cgggtgataa ggagatcatc acaatggcat ttaagatctg gcaaattggt
    16801 ttgcatttac aacagcaaga agcggtagcg gttgcgatcg tgcggggcgc aaaagaatgc
    16861 tttttgcaac gctggtggcg gttgccgctg gagaacgaca ttatcaaaga tgggcggatt
    16921 gttgatgcgc agcagctggc taaaacgttg ttaccttgga gtcgcgaact gccgcagcgt
    16981 catcacatta tgttggcgtt tcccgccagt cgcacattac agcggtcatt tccgcgcccg
    17041 tcgatgtccc ttggtgagcg ggagcaaacg gcctggctgt cagggacgat ggcccgcgag
    17101 ctggatatgg atccggactc cctgcgcttc gattacagcg aagactcact cagtcccgcc
    17161 tataacgtga ctgccgcgca aagcaaagag ctggcaacgc tgcttactct ggcagaaagg
    17221 ttgcgtgttc atgttagtgc gatcaccccg gatgccagtg cattacagcg attcctgcct
    17281 tttttacctt ctcatcagca atgtctggcc tggcgtgata acgaacagtg gctgtgggcg
    17341 acgcgctata gctgggggcg caaactggcg gtggggatga ctagcgcgaa ggagctggcg
    17401 gcagcgttat tcgttgatcc cgacagcgtc gcgatatgtg gcgaaggcgg atttgacccc
    17461 tgggaggccg tttctgttcg tcagccgctg ctaccgccgc ctggtggaga ctttgccatc
    17521 gcgctggggc tggcgcttgg gaaggcgtac tgatgaaccc gccaattaat tttttgccct
    17581 ggcgacagca acgccggacc gcttttctgc gtttctggtt gctgatgttc gttgcgcctc
    17641 tgctgctggc cgtcgggata acgctaatac tgcgtctgac aggcagcgcc gaagcgcgca
    17701 tagacgcagt tttgcttcag gcggaacaac aactcgcccg cagcttacag ataacgaagc
    17761 cacgtttgct ggagcaacag cagttacgca aacagcgttc gcaacggcag cgccagcgac
    17821 aatttacccg cgactggcaa tctgcgctgg aagcactggc ggcgctttta cccgagcacg
    17881 cctggctgac aacgataagc tggcagcagg gaacgctgga gatcaagggg ctgacaacaa
    17941 gcattaccgc gttaaacgca ctagaaacgt cactccgcca ggatgcgtct tttcatctca
    18001 atcagcgggg agccacgcag caggatgcgc agggacgctg gcaatttgag tatcagttaa
    18061 caaggaaggt tagcgatgaa catgttcttt gactggtggt tcgccacatc accccgcctc
    18121 cgccagtttt gctgggcatt ctggttgctg atgttagtta cgctcatttt tctgtcatcg
    18181 acacaccatg aagagcgcga cgcattaatt cgattacggg caagtcatca ccagcagtgg
    18241 gccacactgt atcgcctggt tgacaccact cccttcagcg aagaaaaaac gctgcccttt
    18301 tcgccactgg attttcagtt gcccggcgcg caactggttt cctggcatcc atccgcgcag
    18361 ggaggtgagt tggcgttgaa aacgctgtgg gaagcagtgc cgtcggcatt cacacggctg
    18421 gcagagcgca acgtcagcgt gagccgtttt tcgttaagcg tggaaggtga tgatcttttg
    18481 ttcacgctac aactggagac gccgcatgag ggttaaacgc tggttgttgg caggtattgc
    18541 attgtgcctt ttaaccggta tgcgtgaccc ttttaaaccg ccggaagatc tatgccggat
    18601 tagcgaactt agccagtggc gctatcaggg gatggtaggg cgaggcgagc gcatcatcgg
    18661 tgtaataaaa gacgggcaaa agaaatggcg acgggtgcag caaaacgatg tgctggaaaa
    18721 cggctggaca attttacagc tgacgccaga cgtactaacg ctgggtaccg ggacaaactg
    18781 cgaaccgcca caatggttgt ggcaacggca aggagataca aatgaagcaa tggatagccg
    18841 cactactgtt gatgctgata cccggcgtac aggcggcaaa gccgcaaaaa gtgacgctga
    18901 tggtggatga cgttccggta gctcaggtgt tgcaggcgct ggctgaacag gagaagttga
    18961 acctggtcgt gtcgccagac gtcagcggta cggtgtcgtt acatctaaca gatgttccct
    19021 ggaagcaggc actacaaact gtagtgaaaa gcgccggact gataacgcgg caggaaggca
    19081 acattctctc agtgcattcc attgcctggc agaataacaa tatcgcccgc caggaggcgg
    19141 agcaggcgcg ggcgcaggca aatctgccgc tggaaaatcg cagtataacc ctgcaatacg
    19201 ccgacgcggg agaactggcg aaagcggggg agaagctact gagtgccaaa gggagtatga
    19261 ccgtcgataa acgcaccaat cgccttttgc tacgagataa caaaacggcg ttaagcgcgc
    19321 ttgaacagtg ggtagcgcaa atggatctgc cggtcgggca ggttgagctg tcggcgcata
    19381 ttgtcaccat taatgaaaaa agtttgcgtg agttaggcgt gaaatggacg ctggccgatg
    19441 cgcaacacgc tggtggcgtt gggcaagtca ccacgcttgg tagcgacctc tccgtagcga
    19501 cggcgacaac gcatgtcggt tttaacattg ggcgcatcaa cggacgcttg ctggatcttg
    19561 agctttccgc gctcgaacaa aaacagcagc tggatattat cgccagtccg cgtctgctgg
    19621 cctcacatct tcagcctgcc agcattaaac aggggagcga aattccatat caggtttcca
    19681 gcggggaaag tggcgcgacg tcggtggaat ttaaagaggc cgtcctgggg atagaggtca
    19741 cgcccacggt gttacaaaaa ggtcgcatcc ggctgaaatt acacatcagc cagaacgttc
    19801 cggggcaggt gctacagcag gccgatggcg aagtgctggc gattgataag caggagatcg
    19861 aaacgcaggt cgaggtcaaa agcggagaaa cgttggcgct gggcggcatt tttacccgta
    19921 aaaataaatc gggtcaggat agcgtaccgt tgcttggcga cattccctgg ttcgggcaat
    19981 tatttcgtca tgacggaaaa gaatatgaac gacgcgagtt agtggtgttt atcacgccac
    20041 gactggtttc cagtgagtaa acagccgtaa aagcggtaat gtttttatgc tgaacgtgtt
    20101 tcatctattt gacgcgcgca ggtatttagc atacaaggag taccgatttg agagttggcg
    20161 ctcttcgctg cctgcgttcc atgatgatga tttatcattc aggcggcatt ttgctgtctt
    20221 ttttacgcta atcttacccg gtgatttatc gccagagcgg tggtagcaag gcagcgcgct
    20281 tgcagcgacc agatatgcag agggatgggt gatttattca gttgccaaac ccgctggagt
    20341 attgagataa ttttcagtct gactctcgca atatcttatg aggtttcagt tcatgtcctg
    20401 cggcgctctc tgagcgaagc gggtttatca ttaacgaata gtcttagtag taccgaaaaa
    20461 atggcagaga aacgcaatat ctttctggtt gggcctatgg gtgccggaaa aagcactatt
    20521 gggcgccagt tagctcaaca actcaatatg gaattttacg attccgatca agagattgag
    20581 aaacgaaccg gagctgatgt gggctgggtt ttcgatttag aaggcgaaga aggcttccgc
    20641 gatcgcgaag aaaaggtcat caatgagttg accgagaaac agggtattgt gctggctact
    20701 ggcggcggct ctgtgaaatc ccgtgaaacg cgtaaccgtc tttccgctcg tggcgttgtc
    20761 gtttatcttg aaacgaccat cgaaaagcaa cttgcacgca cgcagcgtga taaaaaacgc
    20821 ccgttgctgc acgttgaaac accgccgcgt gaagttctgg aagcgttggc caatgaacgc
    20881 aatccgctgt atgaagagat tgccgacgtg accattcgta ctgatgatca aagcgctaaa
    20941 gtggttgcaa accagattat tcacatgctg gaaagcaact aattctggct ttatatacac
    21001 tcgtctgcgg gtacagtaat taaggtggat gtcgcgttat ggagaggatt gtcgttactc
    21061 tcggggaacg tagttaccca attaccatcg catctggttt gtttaatgaa ccagcttcat
    21121 tcttaccgct gaaatcgggc gagcaggtca tgttggtcac caacgaaacc ctggctcctc
    21181 tgtatctcga taaggtccgc ggcgtacttg aacaggcggg tgttaacgtc gatagcgtta
    21241 tcctccctga cggcgagcag tataaaagcc tggctgtact cgataccgtc tttacggcgt
    21301 tgttacaaaa gccgcatggt cgcgatacta cgctggtggc gcttggcggc ggcgtagtgg
    21361 gcgatctgac cggcttcgcg gcggcgagtt atcagcgcgg tgttcgtttc attcaagtcc
    21421 cgacgacgtt actgtcgcag gtcgattcct ccgttggcgg caaaactgcg gtcaaccatc
    21481 ccctcggtaa aaacatgatt ggcgcgttct accagcctgc ttcagtggtg gtggatctcg
    21541 actgtctgaa aacgcttccc ccgcgtgagt tagcgtcggg gctggcagaa gtcatcaaat
    21601 acggcattat tcttgacggt gcgtttttta actggctgga agagaatctg gatgcgttgt
    21661 tgcgtctgga cggtccggca atggcgtact gtattcgccg ttgttgtgaa ctgaaggcag
    21721 aagttgtcgc cgccgacgag cgcgaaaccg ggttacgtgc tttactgaat ctgggacaca
    21781 cctttggtca tgccattgaa gctgaaatgg ggtatggcaa ttggttacat ggtgaagcgg
    21841 tcgctgcggg tatggtgatg gcggcgcgga cgtcggaacg tctcgggcag tttagttctg
    21901 ccgaaacgca gcgtattata accctgctca agcgggctgg gttaccggtc aatgggccgc
    21961 gcgaaatgtc cgcgcaggcg tatttaccgc atatgctgcg tgacaagaaa gtccttgcgg
    22021 gagagatacg cttaattctt ccgttggcaa ttggtaagag tgaagttcgc agcggcgttt
    22081 cgcacgagct tgttcttaac gccattgccg attgtcaatc agcgtaacaa caagaaaggt
    22141 caggccgctt atcaagcggt ctattagctt caggttaatt gcaacgtggt aagcattaac
    22201 cttttagtgg ggtgttaaat ggatgaattc aaaccagaag acgagctgaa acccgatccc
    22261 agcgatcgtc gtactggtcg ttctcgtcaa tcttctgaac gttctgagcg tactgaacgt
    22321 ggcgaaccgc agatcaattt tgatgatatt gaacttgatg acactgacga tcgccgtccg
    22381 actcgtgcgc aaaaagagcg caatgaggaa ccggaaatcg aagaagaaat tgacgaatcc
    22441 gaagatgaaa ccgtggatga agagcgcgta gagcgtcgtc cgcgtaagcg caaaaaagca
    22501 gccagtaaac ccgcttctcg tcagtatatg atgatgggcg tcggcattct ggttctactg
    22561 ctgttgatca tcggtatcgg ttctgcgcta aaagccccct cgacctcttc cagcgatcaa
    22621 accgcgtctg gcgataagag tattgatctt gcaggcaatg cgaccgatca ggcgaatggg
    22681 gtgcagccag cgccgggaac cacgtctgcg gaaaatactc agcaggatgt ttctctgcca
    22741 ccgatctctt ctacgccgac tcaagggcaa accccggcgg caacggatgg tcaacaacgt
    22801 gttgaagtgc agggtgacct gaacaatgcg ctgactcagc cacaaaatca gcaacagttg
    22861 aacaatgtgg cggtcaattc cacattgccg accgaacccg cgactgtcgc gcctgttcgc
    22921 aatggcaatg catcgcgtga cacggcgaaa acgcaaaccg ctgaacgtcc gtccactacg
    22981 cgcccagctc gtcagcaggc ggtgattgaa ccgaaaaaac cgcaagcaac cgtgaaaacg
    23041 gagccgaagc cggtagcaca gacgccgaag cgtactgaac cagctgcccc tgtggcgagc
    23101 acgaaggcac cggctgcgac ttctacgcca gcaccaaaag agacggcgac tacggctcca
    23161 gtacagacgg catccccggc gcaaaccacg gcaacaccag ccgctggagg gaagaccgca
    23221 ggtaatgttg gttcgttgaa atcggcaccg tccagccatt acactctgca gctgagcagt
    23281 tcctctaact acgacaacct gaacggttgg gcgaagaaag agaatctgaa aaactacgtt
    23341 gtctatgaaa cgacgcgtaa tggtcagccg tggtatgtcc tggtttctgg cgtgtacgct
    23401 tcgaaagaag aggcgaaaaa agcggtatct acattgccag cagatgttca ggccaaaaac
    23461 ccgtgggcga aaccgctgcg tcaggtacag gccgatctga agtaatcaag gttatctccc
    23521 gcaatggttt atcgttgcgg gagctgcctg aagcgctgga tgctgtcgga gctttctcca
    23581 cagccggaga aggtgtaatt agttagtcag catgaagaaa aatcgcgctt ttttgaagtg
    23641 ggcagggggc aagtatcccc tgcttgatga tattaaacgg catttgccca agggcgaatg
    23701 tctggttgag ccttttgtag gtgccgggtc ggtgtttctc aacaccgact tttctcgtta
    23761 tatccttgcc gatatcaata gcgacctgat cagtctctat aacattgtga agatgcgtac
    23821 tgatgagtac gtacaggccg cacgcgagct gtttgttccc gaaacaaatt gcgccgaggt
    23881 ttactatcag ttccgcgaag agttcaacaa aagccaggat ccgttccgtc gggcggtact
    23941 gtttttatat ttgaaccgct acggttacaa cggcctgtgt cgttacaatc tgcgcggtga
    24001 gtttaacgtg ccgtttggcc gctacaaaaa accctatttc ccggaagcag agttgtatca
    24061 cttcgctgaa aaagcgcaga atgccttttt ctattgtgag tcttacgccg atagcatggc
    24121 gcgcgcagat gatgcatccg tcgtctattg cgatccgcct tatgcaccgc tgtctgcgac
    24181 cgccaacttt acggcgtatc acacaaacag ttttacgctt gaacaacaag cgcatctggc
    24241 ggagatcgcc gaaggtctgg ttgagcgcca tattccagtg ctgatctcca atcacgatac
    24301 gatgttaacg cgtgagtggt atcagcgcgc aaaattgcat gtcgtcaaag ttcgacgcag
    24361 tataagcagc aacggcggca cacgtaaaaa ggtggacgaa ctgctggctt tgtacaaacc
    24421 aggagtcgtt tcacccgcga aaaaataatt ctcaaggaga agcggatgaa acagtatttg
    24481 attgccccct caattctgtc ggctgatttt gcccgcctgg gtgaagatac cgcaaaagcc
    24541 ctggcagctg gcgctgatgt cgtgcatttt gacgtcatgg ataaccacta tgttcccaat
    24601 ctgacgattg ggccaatggt gctgaaatcc ttgcgtaact atggcattac cgcccctatc
    24661 gatgtacatc tgatggtgaa acccgtcgat cgcattgtgc ctgatttcgc tgccgctggt
    24721 gccagcatca ttacctttca tccagaagcc tccgagcatg ttgaccgcac gctgcaactg
    24781 attaaagaaa atggctgtaa agcgggtctg gtatttaacc cggcgacacc tctgagctat
    24841 ctggattacg tgatggataa gctggatgtg atcctgctga tgtctgttaa cccaggtttt
    24901 ggcggtcagt cgttcattcc acaaacactg gataaactgc gcgaagtacg tcgtcgtatc
    24961 gacgagtctg gttttgacat tcgactggaa gtggacggtg gcgtgaaggt gaacaacatt
    25021 ggcgaaatcg ctgcggcggg cgcggatatg ttcgtcgccg gttcggcaat cttcgaccag
    25081 ccagactaca aaaaagtcat tgatgaaatg cgcagtgaac tggcaaaggt aagtcatgaa
    25141 taagtttgaa gatattcgcg gcgtcgcttt tgatctcgac ggtacgctgg ttgacagtgc
    25201 tcccggtctt gctgctgcgg tagatatggc gctgtatgcg ctggagttac ccgtcgcagg
    25261 tgaagaacgc gttattacct ggattggtaa cggcgcagat gttctgatgg agcgtgcatt
    25321 ggcctgggcg cgtcaggaac gtgcgactct gcgtaaaaca atgggtaaac cgcccgttga
    25381 tgacgacatt ccggctgaag aacaggtacg tattctgcgt aaactgttcg atcgctacta
    25441 tagcgaggtt gccgaagagg ggacgttttt gttcccgcac gttgccgata cgttgggcgc
    25501 gttgcaggct aaaggcctgc cgctaggcct ggtcaccaac aaaccgacgc cgttcgtcgc
    25561 gccactgctc gaagccttag atattgccaa atactttagc gtggttatcg gcggcgatga
    25621 tgtgcaaaac aaaaaaccgc atccggaccc gctgttactg gtggctgagc ggatgggaat
    25681 tgccccacaa cagatgctgt ttgtcggcga ctcacgcaat gatattcagg cggcaaaagc
    25741 ggcaggttgc ccatcagttg gcttaaccta cggatataac tacggcgagg ctatcgatct
    25801 cagccagcct gatgtaattt atcagtctat aaatgacctt ctgcccgcat tagggcttcc
    25861 gcatagcgaa aatcaggaat cgaaaaatga ctaagcccat cgtttttagt ggcgcacagc
    25921 cctcaggtga attgaccatt ggtaactaca tgggtgcgct gcgtcagtgg gtaaacatgc
    25981 aggatgacta ccattgcatt tactgtatcg ttgaccaaca cgcgatcacc gtgcgccagg
    26041 atgcacagaa gctgcgtaaa gcgacgctgg atacgctggc cttgtatctg gcttgtggta
    26101 tcgatcctga gaaaagcacc atttttgttc agtcccacgt accagaacat gcgcagttag
    26161 gctgggcact gaactgctat acctacttcg gcgaactgag ccgcatgacc cagtttaaag
    26221 ataaatctgc gcgttatgcc gagaacatca acgctggtct gtttgactat ccggtgctga
    26281 tggctgcgga catcctgctg tatcaaacta atctggtacc ggtgggtgaa gaccagaaac
    26341 agcacctgga actgagtcgc gatatcgccc agcgtttcaa cgcgctgtat ggcgagatct
    26401 ttaaggtgcc ggagccgttt attccgaaat ctggcgcgcg cgtaatgtcg ctgctggagc
    26461 cgaccaagaa gatgtccaag tctgacgata accgcaataa cgttatcggc ctgctggaag
    26521 atccgaaatc ggtagtgaag aaaatcaaac gcgcggtcac tgactccgac gagccgccgg
    26581 tagttcgcta cgatgtgcag aacaaagcgg gcgtttccaa cctgctggat atcctttctg
    26641 cggtaacggg ccagagcatc ccagaactgg aaaaacagtt cgaaggcaag atgtatggcc
    26701 atctgaaagg tgaagtggct gatgccgttt ccggtatgct gactgaattg caggaacgct
    26761 atcaccgttt ccgcaacgat gaagccttcc tgcaacaggt gatgaaagac ggcgcagaaa
    26821 aagccagtgt acacgcttcc cgtacgctga aagcggtgta cgaagcgatt ggttttgtgg
    26881 cgaagccgta atatcaaaac gctcggacgg tcccctcgcc cctttgggga gagggttagg
    26941 gtgaggggag caggcaagcg ccagtgggaa ttaccctttg ctcgcattgt cgaataaatc
    27001 acttcgtcat cactctgaaa ccggggaaac ccggtttttt tatcctcatt tgcaataatc
    27061 cgaaaaaatg tgaagcgcct cgccgtttcc acatctcaca gttgccactt atcttcattt
    27121 taatgaagat ataaacattc attttattga aaatatatta tgcgtcgaac gtttatcaaa
    27181 aaagaaggcg tcgtcatcac gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat
    27241 cgactgaaaa ccatagatga gctggcaact gaatgccgtt catcggttgg cctgacgcag
    27301 gccgcgttga aaacgttgga atcaagtgga gcgatacgga ttgaacgccg tgggcgcaat
    27361 ggcagttatc tggtcgaaat ggataacaaa gcgctgctga gtcatgtgga tatcaataac
    27421 gttgtgtgtg ccatgccttt gccctatacc cgcttgtacg aaggtttagc aagcgggctg
    27481 aaagcccagt ttgatggcat tcctttttat tatgcgcata tgcgtggcgc ggatattcgt
    27541 gtggagtgtt tgcttaacgg tgtctatgac atggcagtag tttcccgcct ggcggcggaa
    27601 agttatctca gccaaaacaa tttgtgcatc gccctcgagc ttggcccgca tacgtatgtt
    27661 ggtgaacatc aactgatatg ccgcaaaggt gagtctggaa atgttaagcg agtaggtctc
    27721 gatagtcgtt cggcggatca gaaaatcatg accgatgtct tttttggcga tagtgatgtg
    27781 gaacgtgtcg atctctctta tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat
    27841 gcggttatct ggaacgtggt ggcggaaaac gaactgacca tgctgggatt agaggcgacg
    27901 ccgctcacag acgatccgcg atttttacag gccaccgaag ccgtggtcct gacgcgagtc
    27961 gatgattacc caatgcaaca actgctgcgt gccgttgtag ataaacacgc cctgctcgcc
    28021 catcaacaac gggtagtgag tggggaacag gaaccgagtt attaaacgaa aggcatctga
    28081 tatggaaacc agactcaacc tgctttgcga ggcaggcgtt attgataagg acatctgcaa
    28141 aggcatgatg caggtcgtca acgtactgga aacagagtgc catctgccgg tgcgcagtga
    28201 gcaaggaacg atggcgatga cacatatggc gagtgcactg atgcgcagtc gccgtggtga
    28261 agaaatagag ccgctggata acgaattgct ggcagagctg gcgcaatcca gccactggca
    28321 agccgttgtg caattgcatc aggtgttgtt gaaggaattc gcactggaag ttaatccgtg
    28381 tgaagaaggc tatttgctgg ccaaccttta tggattatgg atggctgcta acgaagaggt
    28441 ttgaagctcc cgacaaatcc acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa
    28501 ttcaaggaac agaaacgagg caacaagatg tttgtagaag cattgaaacg ccagaacccg
    28561 gcgctgattt ccgccgcact aagtttgtgg cagcagggca agatcgcccc ggacagctgg
    28621 gtgatcgacg tggatcaggt actggaaaac ggtaagcgac tgattgagac ggcgcggctt
    28681 tacggcattg aactgtatct gatgaccaag cagtttggcc gtaacccatg gctggcggaa
    28741 aaattgctgg cattaggtta cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc
    28801 atgcgccgcg ctggtttgtc ggtggcgcat caggggcatc tggtacaaat cccttgtcat
    28861 caggttgctg acgccgttga acagggcacc gacgtcatca ccgtgtttac tctcgacaaa
    28921 gcgcgggaag tttctgcggc agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa
    28981 gtttatagcg acgatgattt tctttatccg gggcaggaga gcggttttgt acagcattcg
    29041 ctgcacgagg tggttgctga aattaagaaa ctgccgggtc tgcatttagc cggacttacc
    29101 catttccctt gcctgctttg ggatgaggct gccggaaaag ttttgccgac accgaatctt
    29161 cacacgctga tacaggcacg ggatcaactg gcgaaatctg gtattgcact tgagcaactg
    29221 aacgcgcctt cagcgaccag ctgcacttcg ctgccattac tggcgcaata cggtgtgact
    29281 catgccgaac ccggtcatgc gctgacgggc actattccgg caaaccagca gggcgatcag
    29341 tctgaacgta tcgcgatgct ctggttgagt gaaatctccc atcatttccg tggcgacagc
    29401 tactgctacg gcggcggtta ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg
    29461 ccagaaaatc aaaagattac tgaaaccaat ctcaaaactg tggatgacag cagtatcgac
    29521 tacaccctgt cgctggcagg cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc
    29581 acgcagattt ttgtcacccg tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa
    29641 ccgaaaatcg ttggtcgtta tgacagtctt ggaaactctc tgggggcgta atggcgcgat
    29701 ttgtggtgtt agtgattgat agctttggcg taggggcaat gaaagatgtc acgctggtgc
    29761 gtccgcaaga tgcgggagcg aatacatgtg gtcacatcct gagccagttg ccgcatttgc
    29821 agctaccaac gctggagaag ctggggctaa tcaacgcatt gggttatgcg ccaggcgata
    29881 tgcagccgtc agattccgca acctggggcg tggcagagct gcaacatgaa ggtggcgata
    29941 cctttatggg gcatcaggaa attttaggca cgcgcccgtt accgccgctg cggatgcctt
    30001 tttgcgatgt gattgaccgt gttgagcagg cattagtatc cgccggttgg caggtggagc
    30061 gccgtggcga tgaactgcaa tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc
    30121 tcgaggcgga tttaggccaa gtctataaca ttaccgccaa tctctctgtg atctcttttg
    30181 acgacgcaat caaaattggt cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta
    30241 catttggtgg cctgttaacc gacagtcaac gcattctcga tgccgcagaa agcaaagaag
    30301 ggcgctttat tggtatcaat gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg
    30361 tgcatatggg ctacggtgtc gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg
    30421 gcgtgccaac cgtgctggtg ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga
    30481 gctggcaaaa cctggtggat agccagcgga ttatggatat caccctcgac gaatttaaca
    30541 cccatccgac ggcgtttatt tgcaccaata tccaggaaac cgacctcgct ggtcatgcag
    30601 aagacgtcgc acgttatgcc gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg
    30661 ttgatgcgat gcagccagat gattgcctgg tcgtgatggc ggatcacggc aacgatccga
    30721 ccattggtca cagccaccat acccgcgaag tggtgccgct gctggtttat cagcaaggcc
    30781 tggctgctac gcagctcggt gtgcgcacca cgctttctga tgtgggagct accgtgtgtg
    30841 aatttttccg tgcgccaccg ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg
    30901 caggagacac cctatgagtt ttgatccgac gggttacacc ctggcccatg agcatctgca
    30961 tattgatctc tccggcttta aaaacaacgt ggactgccgc cttgatcagt atgcgttcat
    31021 ttgccaggag atgaacgacc tgatgacccg gggtgtacgt aatgtgattg agatgaccaa
    31081 ccgttacatg gggcgcaatg cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa
    31141 cgtggtggcc tgtactggtt attaccagga tgcgtttttc ccggaacatg tggtgacccg
    31201 cagcgtgcag gaactggcgc aggagatggt cgatgaaatt gaacagggta tcgatggcac
    31261 ggagctgaaa gccgggctca tcgcggagat cggctccagc gaaggaaaga ttacgccgct
    31321 ggaagagaag gtatttattg ccgctgcgct ggcgcataac cagaccggac gcccgatctc
    31381 cacgcatacg tcgttcagca ccatggggct ggaacaactg gctctgttgc aagcccacgg
    31441 agttgatctt tcgcgcgtca ccgttggtca ctgcgatctg aaagacaacc tcgacaacat
    31501 tttgaagatg atcgatctcg gcgcgtacgt gcagttcgac accattggta agaacagtta
    31561 ctacccggac gaaaagcgca ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa
    31621 ccgcgtcatg ctgtcgatgg atattacgcg ccgctcccat ttaaaagcca atggtggtta
    31681 tggctatgac tatttattga ccacctttat tccgcaattg cgccagtcag gattcagtca
    31741 ggccgatgtg gatgtgatgt tacgtgaaac tccctctcaa tttttccaat aaggacagac
    31801 tcatgaaaaa gattggcgtt gcaggcttac agcgtgagca gattaaaaaa accattgaag
    31861 cgacggctcc tggcagtttt gaagttttca ttcacaacga catggaagcg gcaatgaaag
    31921 tgaaatccgg gcaactggat tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat
    31981 tgtcgattgc catcgcggtg attggctata acaaaagttg caccattgcc aaaccaggca
    32041 ttaaagcgaa agacgagcat atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc
    32101 tttccgttga gcacgtcgaa cacgcgattc cgatgctgat taaccatctg aaataaaagg
    32161 cacgactatg gatctgtata ttcagattat cgtggtggcg tgcctgacgg gtatgacatc
    32221 gcttctggcg catcgctcgg cggctgtttt tcatgacggc atccgcccga tcctgccgca
    32281 actgattgaa ggctatatga accgtcgcga ggcggggagt atcgcttttg gtctgagcat
    32341 tggttttgtg gcctcggtgg ggatctcttt taccctgaaa accgggctgc tcaacgcatg
    32401 gttactcttt cttcctaccg atatcctcgg cgtactggcg ataaacagcc tgatggcgtt
    32461 tggtcttggc gctatctggg gcgtgttgat ccttacttgc ctgttgccag taaaccagct
    32521 gctgaccgcg ctgccggtgg atgtattagg tagcctcggg gaattaagct cgccggtagt
    32581 ttctgctttt gcactgttcc cgctggtggc gattttctac cagtttggct ggaagcaaag
    32641 tctggtcgcc gccgtggttg ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca
    32701 tcttaaccct gaatccatcg aaatctttat tggcatggtg atgctgctgg ggatcgcgat
    32761 aactcacgac ctgcgtcatc gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt
    32821 tgaagaacgc acgtcgcgga ttatcaaaaa cttaccctat atcgccatcg tgggagcatt
    32881 gattgccgcc gttgccagca tgaagatttt cgccggcagt gaagtgtcga tcttcactct
    32941 ggagaaagcg tactccgcag gcgtaacgcc ggaacaatcg caaacgctga tcaaccaggc
    33001 ggcactggca gaatttatgc gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt
    33061 agcaacgggt gtgtatgcag ttgcgggctt tacctttgtt tatgcggtgg gctatctctc
    33121 gccgaatccg atggttgcag cggtattagg cgcagtggtt atttcggcgg aagtcttgct
    33181 gcttcgttct atcggcaaat ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa
    33241 catccgtaac gccatgaata tgctgatgga agtggcgctg ctggtcggtt cgattttcgc
    33301 agcaattaag atggcgggtt ataccggatt ctctatcgcg gttgccattt acttcctcaa
    33361 cgaatccctg ggccgtccgg tacagaaaat ggcggcaccg gtcgtggcag taatgatcac
    33421 cggtattctg ctgaatgttc tttactggct tggcctgttc gttccggctt aaggaaggca
    33481 cctatgaaga cgtttcctct gcaaagcctg acgcttattg aggcgcagca aaagcagttt
    33541 gcgctggtgg acacgatttg ccgtcacttt cccggcgcgg agtttctaac cagcggtgat
    33601 ttgggcttaa cgccggggct gaatcaaccg cgtattaccc aacgggtgga gcaggtgctg
    33661 gctgatgcat ttcacgcaca ggctgcggcg ctggtgcagg gcgcggggac tggcgcgatt
    33721 cgcgccgggc tggcagcttt gctcaaaccg gggcagcgtc ttctggtgca tgacgcgcct
    33781 gtttacccga cgacacgggt tattattgag cagatggggc tgacgcttat tactgttgat
    33841 ttcaatgacc tgtcggcact gaagcaggtc gtcgacgagc aacaaccgga tgcggcgctg
    33901 gtgcagcata cgcgccagca gccgcaggac agctacgtgc tggcagatgt gctggcaacg
    33961 ttgcgcgcgg caggtgttcc agcgttaacc gatgacaact atgcggtgat gaaggtggcg
    34021 cgaatcggct gtgaatgcgg cgcgaatgtc tcgacatttt cctgcttcaa gctatttggg
    34081 ccagagggtg ttggtgcagt ggtcggcgat gctgatgtta tcaaccgtat tcgcgccacg
    34141 ctttactccg gcggtagcca gatccagggc gcacaggcgc tggaagtatt gcgttgtctg
    34201 gtgtttgcgc cagtgatgca cgcggtgcag gcaggggtat ctgaacggtt gctggctttg
    34261 cttaacggtg gtgaggtgcc ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag
    34321 gtgttgattg tcgagtttca tcagccgatt gccgccagag tgctggaaga ggcgcaaaag
    34381 cgcggtgcct tgccttaccc ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt
    34441 tatcgccttt ccggaacgtt tcgccaggcg aatccacaat cagaacattg tgcgattcgc
    34501 attaacccga atcgcagcgg tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc
    34561 agtatttaat gctggatagc accgtcaagc taaattccgt actgaacggt cccctcgccc
    34621 ctttggggag agggttaggg tgaggggaac gtacgggatg taaaattcaa cgcgccagca
    34681 tcgccggatg cgatgctggc gcatcttatc cggcctacgg ttaatgtctt ttattatcaa
    34741 tagttaaggt gatgcgattg gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat
    34801 tctgatcaaa ggcgattttg ctgatgcgaa acaacggttc gcccagttcg catttcagcc
    34861 attttgcctg cgggcgggtg gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc
    34921 gcgtgtcaaa acgctcctga aataactgat aggtggagct tccttcgacg taaatctcat
    34981 caaagtcagg ataacgcgac agcgggatcc atgaactatc aataaacagc ggctctttat
    35041 cgagatacat cacccggcag agatggaaca cttcgctgtt tccggggatg ttcagctttt
    35101 tgcaaaacgg cgcggcgctg acccgttcct gttcgatcac tttctctttc gtcgccttgc
    35161 cttgtgagac gccaaaatcg gtaaaaccac tgacagtaag cagggcgttt tcaactttct
    35221 ggctttgtac aaaggtgcct tttccctgcc agcggatcag tacgccgtcc gccactaagt
    35281 cgctgatggc tttgcgaatg gtaatgcggc tgacgttata ttgtgtacaa agctcgtttt
    35341 cggtagggat ctgttgcccg gcctggtaaa ccccctgcgc gatatcatcc agcagtcgct
    35401 ggcggacggt agcgtagagg agttgatgag agtagcggtc cgtagctgac ataatcgacc
    35461 ctgaaataaa gaatgaatta ctcggattat agggtcgttt tccggcaagt aacagcacgt
    35521 tggcgtgaag atgctcacgc caacgttata cctaccaggc accgtggtac tgaatggttt
    35581 tcgccgcgca cgccgttccc tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc
    35641 cgcaaaggaa tccggcaatg aacgaatctc cggcacccat ggtgtcgata accgtcaccg
    35701 gttcaggagc ctgacgccag aactgcgcgc catcccaggc aatgctgccg ttttcaccca
    35761 gcgtgacaat cactgttcct gcgccacggg caacaatcgc tttcatcttc agacgcagcg
    35821 tttcgtcttc ttgcggtgcg gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc
    35881 agagcgggct gtcccacttg tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca
    35941 gctgtgggaa tgcgtcttcc gcatgtcccc agattgccgc gtgcacaatg tcatactgcg
    36001 ccagccaggc gtaatcctct tcactcaggg caaagtcggc catcacgcct tcggtgtagt
    36061 cgccaaaaac gcgatcattg tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt
    36121 tcgtatggac atggctgata tcgacgccca tgcgggcgag atcctgcttc agctttgtgc
    36181 cgtagtcatc gtcacccacc caggtaatgc atcccggctg tatgccgtag cgagtgcagt
    36241 acaccgccac attgaccgca ttaccgccag aaaacgcttt attcagttgc gggtagatat
    36301 cgacgcagtt atcgccgatt gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc
    36361 gcgaaagcgt tccagcgcct ggcgggcata gagtctgggc tcgttcatat acatcgtcac
    36421 cagctccacc gtacagtaac cctcatagcc ccgctcaata atatcgcgca tcagttcccg
    36481 cagcggcatt ttgccttctc caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac
    36541 aatatgcaga tgacgtaatt tatcgcccag tttgtcgaaa taactcatca ccggttccgc
    36601 ctggacatac ggcgcgcaaa tgtcgaccat gctgaacagg cgcggcgaag gcaccagcgc
    36661 cagcgcatga agcacatcat tagcattaca cacgatgttc gattcatacg gcgttaacgg
    36721 ttcgaggatt agatccatgc cgatgttttc cgcgtactca tacagttcgc ttaggttctc
    36781 tgccagccgt ccccagataa cattaggtgg cgtgagatag cccgcgtggg ccgcggaaat
    36841 cagcgtataa cccgcgttca tctcttttgc catatccatc gccagcttga tcatgtcgag
    36901 gctttcgcga cgcatatgtt catcgcccag catcatgtta tacggatagc cattggtttc
    36961 tggcgtatag ccgataatcg gcatctgata cgtctgcgcc agcgccttga tttgtttgat
    37021 gccgcccgct tttaagtccg gcgcgaacgc gtgcgggcga ccgccccaaa tttcgatgcc
    37081 gtcgtaaccc agctcgcttg catcacgaaa tgcgtgttca ataggcagcc gctggtggcc
    37141 gcaggtaaac atacctgttt ttatcgggta actccttgcg taaatccggc ccgttgccgg
    37201 gccgggcgag ggattaatat tccaccagac caccgtaata gcggcgttca tccgggttgt
    37261 gatctttgta aatagacagg tagtagcaga gccactccat tggcacgaac atcaggaacg
    37321 gtgccagcca cgggtgcagc ccttgcgaaa tttcggcgta atcgatgacg atcacgttgt
    37381 cagtacgctg tttaacaaag ttaatggcgc gttcggtggt gtggcgactt tcatcattgc
    37441 cgagcaggaa caggaacgga acgcccggtt cgacaatctc cagcgggcca tggcggaact
    37501 ctccgctctc aatcacgcag ccgtgcgtcc aggtaaattc catcagcgtt acaatgcctt
    37561 ctttgtaacc cagcggacgc agcggacccg cagcaacggt ataaatcatc ggccactggc
    37621 tggccagttc accaagctgg cggccttttt cttcccaggt gcgtaccaga tgaccgagcg
    37681 cattcggcaa ctgcttgaga tcgtttttga tcttgccgat ttccgcgttc ggcgcgaggc
    37741 gggtgatcat ctccagcacc acgctgtagc agagcagcag gtgaatttcc cagatacagt
    37801 cggcctgata atcaatgcta aattccgccg ccgaggtaat cgggctgtcc gcgcgtttgg
    37861 tgaacgccgc agtgagtgcg ccgcaggccc ggcccagctc cagcgctttg attacctctt
    37921 ccgttttacc gtagtcagaa acgccaatca ccgcgcaacg atcgtcgagg cgatacgggg
    37981 tgttatcgca gaactcccag ccggaaatgg cgtagacctg aagatcggaa aagcgatccg
    38041 ccagatgttt cgccgcttgc gccgcgttga gtggcgagcc gcaggcaacg aaataaatac
    38101 gatcaatgtc gcgcttcacc atctcttcca cgatggcgtg caccagcgga acgtcatggc
    38161 tgagaacttt ttccacttcc tgaaccatat tttcggtgac cagaaagtcc acggtgcttt
    38221 tatcaatatc caacatttta ttttctccag ataacattac gacaaagcgt tgagctggcg
    38281 gctacgcttc gcccagaagg cgtaagcagg cagaccagta gcaataacga tgacggcgca
    38341 gataaggccg ggaatcggtg cccagacaaa ggttgaggcg accagaatga ggcttgacgc
    38401 aatggcgagg gtggtcatca gcccgaaagc aggagtacgc cacagcggtt tgtaatcgtc
    38461 gcgtttacga caccagatga tggagccgaa ggtgagggta tttttgaaac acatcaccag
    38521 ggtgaaataa cccagcaggc tggtgagatc ggaaacgaag atgaagaaga gccccagcgc
    38581 cccttgcagg atgatggaga catccggcgt gttgtatttc ggatgcacat ggccgaagca
    38641 tttaaagaac aggttgtctt tcgccatcgc gtattccagc cgcggctggt acatcacgca
    38701 gctggaaagc gaaccaagaa tgacgatcat cgccgtgatg gcaacaaaga tcccagcggt
    38761 gctgccgagt gcggggatcc aggtcagggc gtcggaaatc ggcgtttcag aattggcgag
    38821 tttgtcgaag ggcatcaggt cggaaatcac cagcgccagc agggtgtaga gcaccagaac
    38881 cagcagacag gaaccaatca gcgctcgtgg catggttttt ccggggtttt taatttcgcc
    38941 ggtcatataa cagatagagg ccatgccggt atacgaccaa ctggtggcag agatccccgc
    39001 cagcagcgcc ataaagctgc ccgttgcgcc aatcgcagtg gtggtagggg cggcaaaatt
    39061 ctccgcttta aaccagaaga tcccaaggcc aatgacgata gtgaacggga taattttggc
    39121 gatggtaatt agcgtctgaa acgctgcgcc gccttcaacg gagcgcaggt gtagcaacat
    39181 aaaggcgata attaatccgg cggcgataaa tttaccgggc aacggatcga taggcgttaa
    39241 aaagccaaga ttgctgacaa tcgccagcgc cataatcgac aatgacggcg catcgttggc
    39301 ccagaagctg gcccagccgg agaggaaagc cagcggtcgg cttccggcat ttttcagata
    39361 aacataatct gcgccatttt ccggataagc ggtggatagt tccgcataga cgcacatttg
    39421 cgggatcaca attaacccgc caatgacaaa cgtgagcacc gtaagccacg gcgtgcccgc
    39481 tgcttttgcc acttcaccca cagatacaaa aataccggag ccgacggttg tcccgacggc
    39541 gattgcaaga acggcccaaa atccgagctt gcgttggagt tcctggcttc ccatagcgat
    39601 acctttttct tgtccaacac gttgtagggg agagtttgca gtcatttaaa aggtaacatg
    39661 acaatgcatg atgaatataa taacatatga tgttatgtaa tgcggaggaa gatcacaaaa
    39721 tttcgcacag gatcgcgctg tggctaatga atgtagttat ccaattgaaa ttaaagtga
//
LOCUS       DAJTNN010000014        38882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTNN010000014 DAJTNN010000000
VERSION     DAJTNN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.014"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            7..438
                     /locus_tag="OO150_000683"
     CDS             7..438
                     /locus_tag="OO150_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5514930.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFA
                     VQSGPMLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            521..1423
                     /locus_tag="OO150_000684"
     CDS             521..1423
                     /locus_tag="OO150_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR5514931.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKSGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1468..3630)
                     /gene="uvrD"
                     /locus_tag="OO150_000685"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1468..3630)
                     /gene="uvrD"
                     /locus_tag="OO150_000685"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5514932.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCLPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3714..4430)
                     /gene="yigB"
                     /locus_tag="OO150_000686"
     CDS             complement(3714..4430)
                     /gene="yigB"
                     /locus_tag="OO150_000686"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5514933.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4430..5326)
                     /gene="xerC"
                     /locus_tag="OO150_000687"
     CDS             complement(4430..5326)
                     /gene="xerC"
                     /locus_tag="OO150_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5514934.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5323..6030)
                     /locus_tag="OO150_000688"
     CDS             complement(5323..6030)
                     /locus_tag="OO150_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5514935.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6027..6851)
                     /gene="dapF"
                     /locus_tag="OO150_000689"
     CDS             complement(6027..6851)
                     /gene="dapF"
                     /locus_tag="OO150_000689"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5514936.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6896..7090)
                     /locus_tag="OO150_000690"
     CDS             complement(6896..7090)
                     /locus_tag="OO150_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5514937.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGMVHPR"
     gene            7553..7873
                     /gene="cyaY"
                     /locus_tag="OO150_000691"
     CDS             7553..7873
                     /gene="cyaY"
                     /locus_tag="OO150_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5514938.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7913..10459)
                     /gene="cyaA"
                     /locus_tag="OO150_000692"
     CDS             complement(7913..10459)
                     /gene="cyaA"
                     /locus_tag="OO150_000692"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5514939.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLCGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10846..11787
                     /gene="hemC"
                     /locus_tag="OO150_000693"
     CDS             10846..11787
                     /gene="hemC"
                     /locus_tag="OO150_000693"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5514940.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11784..12524
                     /gene="hemD"
                     /locus_tag="OO150_000694"
     CDS             11784..12524
                     /gene="hemD"
                     /locus_tag="OO150_000694"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5514941.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            12546..13775
                     /gene="hemX"
                     /locus_tag="OO150_000695"
     CDS             12546..13775
                     /gene="hemX"
                     /locus_tag="OO150_000695"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5514942.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            13778..14974
                     /gene="hemY"
                     /locus_tag="OO150_000696"
     CDS             13778..14974
                     /gene="hemY"
                     /locus_tag="OO150_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5514943.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15653..15814
                     /locus_tag="OO150_000697"
     CDS             15653..15814
                     /locus_tag="OO150_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514944.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15898..17133)
                     /locus_tag="OO150_000698"
     CDS             complement(15898..17133)
                     /locus_tag="OO150_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5514945.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17280..17356)
                     /locus_tag="OO150_000699"
     tRNA            complement(17280..17356)
                     /locus_tag="OO150_000699"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17320..17322),aa:Pro,seq:tgg)
     gene            complement(17399..17485)
                     /locus_tag="OO150_000700"
     tRNA            complement(17399..17485)
                     /locus_tag="OO150_000700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17449..17451),aa:Leu,seq:cag)
     gene            complement(17506..17581)
                     /locus_tag="OO150_000701"
     tRNA            complement(17506..17581)
                     /locus_tag="OO150_000701"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17546..17548),aa:His,seq:gtg)
     gene            complement(17640..17716)
                     /locus_tag="OO150_000702"
     tRNA            complement(17640..17716)
                     /locus_tag="OO150_000702"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17680..17682),aa:Arg,seq:ccg)
     gene            complement(17819..19204)
                     /locus_tag="OO150_000703"
     CDS             complement(17819..19204)
                     /locus_tag="OO150_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5514946.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAVGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19395..20135)
                     /gene="rffM"
                     /locus_tag="OO150_000704"
     CDS             complement(19395..20135)
                     /gene="rffM"
                     /locus_tag="OO150_000704"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5514947.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20138..21490)
                     /gene="wzyE"
                     /locus_tag="OO150_000705"
     CDS             complement(20138..21490)
                     /gene="wzyE"
                     /locus_tag="OO150_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5514948.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21487..22566)
                     /gene="rffT"
                     /locus_tag="OO150_000706"
                     /gene_synonym="wecF"
     CDS             complement(21487..22566)
                     /gene="rffT"
                     /locus_tag="OO150_000706"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5514949.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRVLAIAAGEVA"
     gene            complement(22563..23813)
                     /gene="wzxE"
                     /locus_tag="OO150_000707"
     CDS             complement(22563..23813)
                     /gene="wzxE"
                     /locus_tag="OO150_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5514950.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23815..24945)
                     /gene="rffA"
                     /locus_tag="OO150_000708"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23815..24945)
                     /gene="rffA"
                     /locus_tag="OO150_000708"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5514951.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24950..25624)
                     /gene="rffC"
                     /locus_tag="OO150_000709"
                     /gene_synonym="wecD"
     CDS             complement(24950..25624)
                     /gene="rffC"
                     /locus_tag="OO150_000709"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5514952.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25602..26483)
                     /gene="rfbA"
                     /locus_tag="OO150_000710"
     CDS             complement(25602..26483)
                     /gene="rfbA"
                     /locus_tag="OO150_000710"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5514953.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLVQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDQERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26502..27569)
                     /gene="rffG"
                     /locus_tag="OO150_000711"
     CDS             complement(26502..27569)
                     /gene="rffG"
                     /locus_tag="OO150_000711"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5514954.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27569..28831)
                     /gene="wecC"
                     /locus_tag="OO150_000712"
     CDS             complement(27569..28831)
                     /gene="wecC"
                     /locus_tag="OO150_000712"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5514955.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28828..29958)
                     /gene="wecB"
                     /locus_tag="OO150_000713"
     CDS             complement(28828..29958)
                     /gene="wecB"
                     /locus_tag="OO150_000713"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5514956.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELVANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30014..31060)
                     /gene="wzzE"
                     /locus_tag="OO150_000714"
     CDS             complement(30014..31060)
                     /gene="wzzE"
                     /locus_tag="OO150_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5514957.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31072..32175)
                     /gene="wecA"
                     /locus_tag="OO150_000715"
     CDS             complement(31072..32175)
                     /gene="wecA"
                     /locus_tag="OO150_000715"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5514958.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32415..33674)
                     /gene="rho"
                     /locus_tag="OO150_000716"
     CDS             complement(32415..33674)
                     /gene="rho"
                     /locus_tag="OO150_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5514959.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33759..33860)
                     /gene="rhoL"
                     /locus_tag="OO150_000717"
     CDS             complement(33759..33860)
                     /gene="rhoL"
                     /locus_tag="OO150_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5514960.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34001..34330)
                     /gene="trxA"
                     /locus_tag="OO150_000718"
     CDS             complement(34001..34330)
                     /gene="trxA"
                     /locus_tag="OO150_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5514961.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34461..35726
                     /gene="rhlB"
                     /locus_tag="OO150_000719"
     CDS             34461..35726
                     /gene="rhlB"
                     /locus_tag="OO150_000719"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5514962.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEILEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35860..37344
                     /gene="gppA"
                     /locus_tag="OO150_000720"
     CDS             35860..37344
                     /gene="gppA"
                     /locus_tag="OO150_000720"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5514963.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37391..>38882)
                     /locus_tag="OO150_000721"
     CDS             complement(37391..>38882)
                     /locus_tag="OO150_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HCR5514964.1"
                     /translation="CNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQDTNT
                     SQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQNYRS
                     SGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAHHFV
                     NKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTN
                     PDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGYEAL
                     TRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQLFSW
                     MTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFPYVY
                     MVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRPEPS
                     RFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9655 a        10585 c         9825 g         8817 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg ttgcgggaga taaagtcggc atcattcgtc tggatgcctt taaaaccagt
      121 aaagagattc agtttgcggt gcagtcaggg ccaatgttat tagaaaacgg cgtaattaat
      181 ccacgcattc acccgaatgt tgcctcgcgt aaaattcgta acggtgtagg gattaataaa
      241 caagggaacg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ttgctttatc ttgatggcac aatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttaagc attgtacact cgaggaggcc agacaaactg ggactggcta
      481 cacagctgga aaatcatcag ataaatgaag ggaaacaaaa atgaaaaaaa cgctcgcaac
      541 gttattttta ttaacgtgtc tgggatcatc atccgcctat gcggataacg ctttgatttt
      601 acaaactgat tttagcctga aagatggtgc cgtctcggcg atgaaagggg tcgcattcgg
      661 cgttgaccat aatcttaaaa tctttgattt aacacacgaa attccaccgt ataacatctg
      721 ggaaggcgct tatcgcctgt atcagactgc cagttactgg ccgcaaggtt cggtgtttgt
      781 tagcgtggtc gatccgggcg taggcactga ccgtaaatcg gtagtgctga aaaccaaaag
      841 cggtcaatac ttcgtttcac cagataacgg tacgctcact ttggtggcac aaactctggg
      901 tattgatagc gtccgggaaa tcgacgaaaa agctaaccgc ctgaaaggtt ctgaaaagtc
      961 atataccttc cacggtcgtg acgtgtatgc ctacaccggt gcacgtctgg cttccggggc
     1021 gattacgttc gaacaggtgg ggccagagct tccagcaaaa gttgtcgaac tttcttatca
     1081 gaaagcaaaa gcaacaaaag gtgaagtgaa gggcaatatc ccgattctcg atattcagta
     1141 cggcaacgtc tggagcaata tcagcgacga gttgctgaat caggcagaaa tcaaactcaa
     1201 cgacacgttg tgtgtgacca tttccgaagg ttcccagcaa aaatacgcag ggaaaatgcc
     1261 gtatgtcgca agcttcggcg atgtgccgga aggccagccg atggtgtatc tgaacagctt
     1321 attgaatgtt tccgtggcgc tgaatatgga taatttcgcg cagaaacatc aggttgcgtc
     1381 tggtgctgac tggaatattg atgtgaagaa gtgcgctaag taaggtgctg atgcggaatg
     1441 cggtgtggcg caccgcatcc cgcaacgtca caccgtctcc aaccgggcat aagccgccac
     1501 cagccattta ataccctggc cctggaatgc cacctgcaaa cggctatgtt caccgctgcc
     1561 ttccatattg acgatggtgc cttcaccaaa cttagcgtgg cgtacacgct ggccgagctt
     1621 gtagccgctg tcgttctcga ccatcggcgt acccatccgc tgatggctga ccgggcggct
     1681 taccgtggcg cgcaggcgca cctcttccac acactcttcc ggcagctcgc caataaagcg
     1741 cgacgggcga tggtaaacct ctttgccata cagacggcga gtttccgcgt aggtcagcgt
     1801 cagtttctgc atcgcgcggg ttacgccaac gtaggccaga cggcgttctt cttccagacg
     1861 cccgccttca tccagcgaca tctggcttgg gaacatgccc tcttccatac cgacgataaa
     1921 cacctgcggg aactccagac ctttcgccga gtgcatggtc atcagttgta ccgcatcctg
     1981 ccaggtatcc gcctgccctt cgcctgcttc cagcgccgca tgggatagga acgcctgcag
     2041 cggcattaaa tcttcgtctt cttcgttgta gctgaactgg cgcgttgccg tcaccagttc
     2101 ctctaagttt tcgatacgcg tctgaccttt ttcgcccttc tcctgctcgt acatggtacg
     2161 caggccggag tctttaatta cccggtcagt ctgtacatgc agcggcatat cggcagtttc
     2221 ctgcgctaag gcgtcgatca gttccataaa ccgctgtaag gcgctggcag cacgtccggc
     2281 gagggctttt tcctgcaaca gttcacgaca tgcctgccag agtgttaact ggcgatcgcg
     2341 cgatgtctga cgtaccacgt ccagcgtccg gtcaccaata ccccgcgttg gcgtattcac
     2401 cacacgctca aaggccgcgt cgtcgttacg gttggcaatc aggcgcagat acgagagcgc
     2461 atctttgatt tcctggcgtt cgaagaagcg catcccgccg taaatacggt acggcatact
     2521 cgcctgcaac agtgcttctt ccagcacacg cgactgggcg ttactgcggt agagaatggc
     2581 gcactcggca agcgccccgc cgttgtcctg ccaggttttg atgcggttaa ccacaaaacg
     2641 cgcttcatcg agttcgttaa aagcgcaata gagggaaata ggctcaccgt ccgcgccatc
     2701 ggtccacagt tttttaccca gacgcccgtt attgttttca atcagggcgt tagcggcgct
     2761 cagaatattg ctggtagagc ggtagttttg ctccagacga atagtttcgg caccggggaa
     2821 atcattaagg aaacgctgaa tattctccac ctgcgccccg cgccagccgt agattgactg
     2881 gtcgtcatca ccgacgatca tcactttgcc ggtgtcgccc gccagcaggc ggatccacgc
     2941 gtactgaatg ttgttggtat cctggaattc gtccaccagg atattggtaa aacgttcgcg
     3001 gtagtgttgc aggatatgcg gcttgttaag ccacaactcg tgagcgcgca gcagcagctc
     3061 ggcgaagtcc accaggcccg cgcggtcaca cgcttcctga tacgcctgat acaccttctg
     3121 ccaggtctgc tccaccggat taccgtagct ttgaatatga tgtggacgca ggccttcatc
     3181 tttctggctg ttgatgtacc acattgcctg gcgcggcggc cactgcttct cgtcgaggtt
     3241 catcgccttg attaggcgtt tgagcaggcg tagctggtcc tcgctgtcga ggatctgaaa
     3301 atcctgcggc agattggcgt ccatatggtg cgcacgcagc agacggtgcg ccagcccgtg
     3361 gaaggtgccg acccacatgc cgccctggct ggtgcccatt agctgcccga tacgatgacg
     3421 catctccgcc gccgctttgt tggtaaacgt caccgccata atcgagtatg gcaagcagtt
     3481 ttccacgctc atcagccagg cgatacgatg caccagtacg cgcgtcttac cactgcccgc
     3541 gcccgccagc accagaaggt tgctgcgtgg cgcggccacc gcttcgcgct gtttgtcatt
     3601 aaggctgtcg agcaggtaag aaacgtccat tggcaccgcc gcgtaaaaat aggttgggcg
     3661 gagaagcgca acgccctccg ccaaaaagct gggtatatat acagatttgc tgattatatc
     3721 agcgaggtca gagatgccaa ccgggaaatt tccagatgcg gcagtaaacg gctgtcccag
     3781 gtttgcatca gatcgccatt ttccggtctg atccaacagg cctgcattcc gctgcgaatt
     3841 gccccaccca cgtcagtggt gagatcgtcc ccaacatgta agatctcgcc gatcggcacg
     3901 ttgagttttt ccgcagccag aaagtacata tcgctgaacg gttttgagcg cccgtgcggg
     3961 ccagcgcgca gcacaaactc aaaataatcc cccaaaccaa acagttccgg ctgggcgtta
     4021 ccgttggtga tcgccaccag cggccatttc ttcgccagct gttttaaggt gtcgtgagtt
     4081 tgctgcggga cgtcgattcg gctgcgccat ttggcaaagt tgatcattgc tgcgtgtgcg
     4141 cctgcactgg cttcttcggc actcagcccg gcgtcgagca tcgcttgttc aatcgaacga
     4201 aaacgccagc gcgtcacgtc gtgataaatc tcgggttccg cttcccgtac cgcctggcgc
     4261 aggcgttgca gatcttcatt ctggaagctg cgcagcgccg gatgataatt ttgcacaaag
     4321 gtaagcgcct ctcgctcggt gcgcaaaatc accggacggt tatcgtaaag ggtatcatcc
     4381 aggtcaaagg tgagcgccga gatgcgcccc aaaggccggt aaaaacgcat tatttccccc
     4441 gtttggcgcg tggatgcgcc gcatcgtaca ccgaggcaag gtgttgaaaa tcaagatgag
     4501 tatagatttg cgtggtggag aggttggcat gacccagtag ctcctgcaca ccacgaagat
     4561 cgccgctcga ctccagcata tgcgtagcga acgagtgacg taatttatgt gggtgaacgt
     4621 gattattcag cccttgtttt atgccccatt cggcaaagcg tttctgcaca ttacgcgcgg
     4681 agatacgctt gcccagtttc gacagaaaaa gcgcgtcgtc ttcgctacca aacaggtcgc
     4741 gcaaatcaag ccagtgctca atccacgcca cagcgttgcg accaatcggc aggcggcgct
     4801 ctttgctgcc tttccccatt acccacactt caccagactc caggtcgagg tgtttgatat
     4861 ccagccccac cagctcggaa agacgcagac cagcgccgta catcacttcc agcattgcac
     4921 ggtcgcgtac agcgagggga tcgttgatat caatatccag cagccgattc atatcgtcga
     4981 cgtcgatatt tttcggcaga tgacgcggcg ctttcggtgc cgaaacacct ttcgccgggt
     5041 tagctttgag ttcgttctgg ctgaccagcc agtcaaaaaa gctacgtagc gcagaaagcc
     5101 gtaacgccag acttgctgct cccagccctt tacggcgact gcgtacagca aaattgcgca
     5161 ccatcgtcac atcacattgc tgccagcttt gcaggccgtt ttcgctggca aaattgatga
     5221 tcgcctcaag ctgacgctgg tagttaagca gggttatcgg gctaagctgg cgctccacgc
     5281 tcagataacg taggtagcgt tctacatcgg tgtgtaaatc ggtcatacgc gttcaatcca
     5341 acgctccaga agctccggca acatcagcgc aatttcatga agtaactgcg ttccttgccc
     5401 ttgttgatag tgactggcat cgcgactggt aaacagcacg acacccaaat cagcatcgct
     5461 tcccagcatc gacatcgcca ccgatcccac cgctttcgct tccggtagca ccaccagcag
     5521 ctctggtccg ttaagcggcc caagatagtg ctgttcctgc cccaaacgct gaatacgcag
     5581 cggttcgaaa gactgacggc ttaatgccag atgagtgtgg ttcgacggcg cacctaagcg
     5641 ccagcgatcc ggaaacaggc gcagactcgc acctgccagg ccgagatcgc gcgcccagcg
     5701 gtgaaagcgc atcagcatat cgtcgagact gctggcggcg gtgagactgc gctgcaggta
     5761 gagtaggcga taaaacaggc cttcgttggc gatagcctgt tccatcaaca gcgccatgtt
     5821 ctcttccaga acatgaatat gattacgtgc gcgggccatg tgccactcga ccaacgaaac
     5881 ggtgccgcgt accggatgtg gcacacgtat cgcttctact gcgcgcgcat tacggataaa
     5941 aaactcagga tttttaatca gataatcgac aaccgcccgg tcatcaagct ccgtgagtgt
     6001 ttcctgcagt tcttcccctg gttgcttcat agatgaataa atccgtcgta gacatgtacc
     6061 gccgggccag tcatatataa cgggtgaccc ggacctttcc aggcgatatc aagacgaccg
     6121 ccggggagtt ccacgcgtac ttcttcggcc agcaaacctt gctgaatccc tactgcaacc
     6181 gccgcacacg cgccgctgcc gcaggcctgg gtttctcctg ccccacgctc ataaacgcgt
     6241 aaacgaatat gctcgcgctt aaccacttgc ataaaaccga tattggcgcg ctccggaaaa
     6301 cgctcgtggc tttccagaac aggaccaagc gtttctaccg ccgcggtatc gacatcatcg
     6361 acctgaatca cgcaatgcgg atttcccatc gacaccacgc cgcataagat tgtctgctcg
     6421 gcggcgcgca taatataggt cttttccgct ttgttagcgc gaaacggcac agcggaaggt
     6481 tcgaagttgg gttcgcccat atttacgcgg accagatcat catcggtgac ggtcagaacc
     6541 atccgcccgt tggcggtgct gacgcggata tcgcgcttat tggtcagccc tttcaggcgc
     6601 acaaaacggg caaagcagcg cgcaccgttg ccgcactgcg ccacttcact gccatcagca
     6661 ttgaaaatgc gatagtgaaa atccagttca ggatcatacg gcggctcaac caccagcagc
     6721 tggtcaaacc ctacccccag gtgccgatcc gccagccgac gaatcagctc cggtgaaaaa
     6781 aagacattct gcgttaccgc gtcgacgacc ataaaatcgt tgccaaggcc atgcattttc
     6841 gagaactgca tcatttactc caatcacgcg ggtacagaaa ctgactttta gtaattcacc
     6901 tgggatggac catcccagtg gcgcgatcgt ttttatccgg caccgtggat tgcgtttgcg
     6961 tctctaccgg tttggtcggc ggcggtgcat ttttatctgc aggcgggaaa tagagcggac
     7021 ctttcagacc gcagcccgtc aggctgaaga gagtaagtaa tacagtgagt accttaaaca
     7081 cgtttttcat tatggattgc ctgtaaagtt catcgctttc tgctttctat catcgcagga
     7141 gaaggcgtaa aagcaagcgt ttagcgactc tttatgaagt ctgaaaagtg aggaaatgct
     7201 gcgagtataa cgcttactcg tgaggattta tgttctgctg gcagtttcgt gatcggcacc
     7261 tcgcgccagg atggctttat gcactatcgc aatgtggttt gtttgttggc ttttttagtt
     7321 ccgtcagcgt gaggatgctc gctcggtaaa cctgatcata atgtttacca gaaacaggga
     7381 aaggcgtggt gtaactgcgc cctaatgaag agacaaatct ttcgccacac agagacatca
     7441 ttattccggt gacggatgaa tcatgccgga acgcagcagc agaggtgcaa actttgaagc
     7501 cctgacccca tgccgctata ctgccgccat cacttaaaaa caggatacaa caatgaacga
     7561 cagtgaattt catcgcctgg ctgatcaact gtggctgacc attgaagagc atctggacga
     7621 ctgggatggc gacagcgata tcgactgcga aatcaacggc ggcgtgctga ccattacctt
     7681 tgaaaacggc agcaaaatca ttatcaaccg ccaggaaccg ctgcaccagg tatggctggc
     7741 aaccaaacaa ggcggctacc attttgatct gaaaggcgat gagtggattt gcgatcgcag
     7801 cggcgaaacc ttctgggatt tgctggaaca ggcggcgacg cagcaggcgg gtgaaacagt
     7861 cagtttccgc taagattgca tgccggataa gcctcgcttt ccggcacgtt catcacgaaa
     7921 aatattgctg taatagcggc gtatcgtgat cctgattggc aggcggcatg ttaccgatag
     7981 attttgtgcg gaacggaatc acctgttcac gaccatcaac cttcacaatc tgatagaact
     8041 gcggcaggtt gaagttgatg aagcttgagc cgtaggtgaa gcggtcgtgc gatgacgagt
     8101 agaagcgact gacgtcacgc accagttcct ctttgctgcc ttcgcagtgg tgatacacct
     8161 caacccggtt gctttcgtca agaatgtaga tattaaagcc attctcgtct tgcgtttctt
     8221 cgaagaagaa ctggatgatc ccttcactag caaagccgtc caccaccgcc ggtaatttga
     8281 cgtgattggt ttcaacctgc actgacaggc cgtgcagttt gttatgcgaa atagcgccat
     8341 aaaactcgat ggcgttctcc agcttctgta ccgatacatt caggcgttcg aagaacaagc
     8401 cccaggtttg accagaaacg cgcagcgcct tgaaacgccc ggtttcctgg cgggtgctgg
     8461 aaaggcgcag ttcaatacac tcagaaacca gttgctgcac gcgagtacgg attaacccgc
     8521 acagatgctg gctataacag aagacttcca cgctatctgg cggtgcggcg tcctgatgca
     8581 ttttgccgag aatagttttc agggcttcga tcatcgattg ctcgccgttg aagtgcagcg
     8641 tacgcacttc gttccacgag ttgcggtaca gcaggtcaac gctacctacc aggcaatttt
     8701 gattctcgcc aaagctgaag acatccagct tacggaaatc aaaatgcacc acctgattgc
     8761 ggaacgccgc tgtcgggtca tattccaggt taacgataat cgccagatgg cggatctcac
     8821 acgggctgta gagcgccttc ggtgtcggtg caggtaagcg cagcgggaaa tggtgcgaca
     8881 cgtcggcgac catctcctgc aacttaggca aatcgacaat gccgttacct ttaatataca
     8941 aacgggtgcg cgaggtcagc aggccgttaa accatgccca cgccaccagt ttgttcaggt
     9001 aacggttata ttccagcggc tgatggctga tgatcgactc aatatttggc gcgcggttat
     9061 acagatacca acctgaacgg ttagcacggc ccggcggcac ataaataaag gtcagattcg
     9121 gttccgagag atcgggtgaa atctgcgggt ttaccagcgt cactttacct ggtaatgctt
     9181 caaacgcggc atacagctta cgggtcagca cgccgatatc ctgcggactg gcggagacgc
     9241 taaggttatt gcgacgcgca aagcggatca gattacggta gctctgcatc atcgcgtcga
     9301 gcaactcgtt gtgcgcctca cgcacctgat caatcttcca gttagcgcgg ttatcgagca
     9361 ttgccagacg agcttcgtcc caaccccact cgctcactaa ctggctcaac actgcgcgac
     9421 gccagcctac gcaggcgcgt tcacggctga gcttttcgca cacttttaaa tagaagcagc
     9481 gacgtactaa atccagacgg gtaaaatctt caatcgccgt caggtattca gtaacacgct
     9541 ccagcatcat gcagtatgga tcgagaccaa acgatacaat ctcaccgtcg tgcaaacgct
     9601 gtttgatatc tttcgccagc agacgtgggt tcgggtattc ccaggaatag gcttccagca
     9661 gcagtgtttt cagtaccgct ttgtatgggg aatcgatact cttgtagagc tgccaaaggc
     9721 tggcaccaaa gtactcttca gcagaaagcg agcttaagcc accgagatcc agccattcat
     9781 ttggcgtcag cacgccctgc gcgtaaagcg tcatcacata gtcgtcgtaa tgctcttctt
     9841 cgtcgcacgg caccatattc cacagaatac gcttaccggc gagacgcacg gcggtacgat
     9901 aaaattcgtc aagcagcagt atatgctggg tggagccaca atcttcgccc cccaagctgc
     9961 cgctttcatt atgacggaag cggttttcat caatcaggaa gaagctgact tccacaccca
    10021 gcgaggcggc ccagctttcc agcagactac atttacgttg tagtaactgg cgctcttcgc
    10081 tatcgagcca ggattgatga cagacccaga tatccaggtc agaggaacaa ctttgcccta
    10141 cggacgaggt gctgcccatg gtgtatacac cagtaattgg aagctcacct ttcggtggat
    10201 cctgtactga cattccacga tacagttcaa gctcgttcag gtagtggcgt tgagtttcat
    10261 caggcgtgta aaggcaaatg cctttgggaa cgttaccatc aaggtaaccc ggcattagcg
    10321 gatggtgata gtgcaacaat gtcggcagta gactgtagac ctgttggaat gcaggcccca
    10381 tagcagcaag cgcgcgatcc acacgcaatt gatttatggc atccagtctc tgtttcagag
    10441 tctcaatata gaggtacaag acgtatcgcc tgatttgcta cccgtcatga ctgtgattcc
    10501 gccaacatca acggtaacac gcggcattcg ggatatttcg tatgtcaaag gtaaccgtta
    10561 ccacttttcg cgcctggttt ttttagtttc acgacgaaaa aatggtctaa aacgtgatca
    10621 atttaacacc ttgctgattg accgtaaaga aagatgcgct acatacaagt gtagcaccgt
    10681 ttattctctg taaattcctt attacgacgg cgtgaaacgc ctgtcaggat ccactgccag
    10741 acctcatttt acggtttgcg caggcgtcta cgtttcacca caacactgac atcactctgg
    10801 caaggatgtt aggatggacc acggatgata atgacggtaa caagcatgtt agacaatgtt
    10861 ttaagaattg ccacacgcca aagcccactt gcactctggc aggcacacta tgtcaaagac
    10921 aagttgatgg cgagccatcc gggcctggtc gttgaactgg taccgatggt gacgcgcggc
    10981 gatgtgattc ttgatacgcc gctggcgaaa gtaggcggaa aaggcttatt tgtaaaagag
    11041 ctggaagtcg cgctcctcga aaatcgcgcc gatatcgccg tacattcaat gaaagatgtg
    11101 ccggttgaat tcccgcaagg tctgggactg gtcactattt gtgagcgtga agatcctcgc
    11161 gatgcctttg tgtccaataa ctatgacagt ctggatgcgt taccagcagg cagtatcgtc
    11221 gggacgtcca gtttacgtcg ccagtgccaa ctggctgaac gccgcccgga tctgattatc
    11281 cgctccctgc gcggcaacgt cggcactcgc ctgagcaaac tagataacgg cgaatacgat
    11341 gccatcattc ttgccgtagc cggactaaaa cgtttaggtc tggagtcccg cattcgcgcc
    11401 gcgttaccac ccgagatttc tcttccagcg gtaggacaag gtgcggtcgg tattgaatgc
    11461 cgccttgatg atgcacgcac tcgcgagctg cttgccgcgc tgaatcacca cgaaactgca
    11521 ctgcgcgtta ccgcagaacg cgccatgaat acccgtctcg aaggcggatg tcaggtgcca
    11581 attggtagct acgccgagct tattgatggt gaaatctggc tgcgtgcgct ggtcggcgcg
    11641 ccggacggtt cgcagattat tcgcggtgaa cgccgcggtg cgccgcaaga tgccgaacaa
    11701 atggggattt cgctggcaga agagctactg aataacggcg cgcgcgagat cctcgctgaa
    11761 gtctataacg gagacgcccc ggcatgagta tcctggtcac ccgcccgtct cccgctggag
    11821 aagagttagt gagccgtctg cgcacactgg ggcaggtggc ctggcatttt ccgctgattg
    11881 agttttctcc gggtcgacaa ttaccgcaac ttgctgatca actggcggcg ctgggggaga
    11941 gcgatctgtt gtttgccctc tcgcaacacg cggttgcttt tgcccaatca cagctgcatc
    12001 agcaagatcg taaatggccc cgactacctg attatttcgc cattggacgc accaccgcac
    12061 tggcgctaca taccgtaagc ggacagaaga ttctctaccc gcaggatcgg gaaatcagcg
    12121 aagtcttgct acaattacct gaattacaaa atattgcggg caaacgtgcg ctgatattac
    12181 gtggcaatgg cggtcgtgag ctaattgggg ataccctgac agcgcgcggt gctgaggtca
    12241 ctttttgtga atgttatcaa cgatgcgcaa tccattacga tggtgcagaa gaagcgatgc
    12301 gctggcaatc ccgcgaggtg acgatggtcg ttgttaccag cggtgaaatg ttgcagcaac
    12361 tctggtcgct gatcccacaa tggtatcgtg agcactggtt actacgctgt cgactattgg
    12421 tcgtcagtga gcgtttggcg aaactcgccc gggaactggg ctggcaagac attaaggtcg
    12481 ccgataacgc tgacaacgat gcgcttttac gggcattaca ataactctca taacaggaag
    12541 ccataatgac ggaacaagaa aaaacctccg ccgtggttga agagaccagg gaggccgtgg
    12601 acaccacgtc acaacctgtc gcaacagaaa aaaagagtaa gaacaatacc gcattgattc
    12661 tcagcgcggt ggctatcgct attgctctgg cggcgggcgt cggtttgtat ggctggggta
    12721 aacaacaggc cgtcaatcag accgccacca gcgatgccct ggctaaccaa ctgacggcat
    12781 tgcaaaaagc ccaggagagc caaaaagccg agctggaagg cattattaag caacaagctg
    12841 cacagcttaa gcaggcgaat cgtcagcaag aaacgctggc aaaacagttg gatgaagtcc
    12901 aacaaaaggt cgccaccatt tccggcagcg atgctaaaac ctggctgctg gctcaggccg
    12961 attttctggt gaaactcgcc ggacggaagc tgtggagcga tcaggacgtc acgaccgctg
    13021 cagcgttgct gaaaagtgca gatgccagcc tggcggatat gaatgacccg agtctgatta
    13081 ccgttcgtcg ggcaattacc gatgatatcg ccagcctttc tgcagtttcg caggtggatt
    13141 atgacggcat catccttaag cttaatcagc tttcaaatca ggtagataac ctgcgtctgg
    13201 ccgataatga cagcgatggt tcgccgatgg attccgatgg tgaagagctt tccagttcca
    13261 tcagcgaatg gcgtatcaat ctgcaaaaaa gctggcagaa ctttatggac aacttcatta
    13321 cgattcgccg tcgtgatgac accgccgtac cgctgttagc gccaaatcag gatatctatc
    13381 tgcgcgaaaa tattcgctct cgcctgctgg tcgcagcaca agctgtaccg cgtcaccagg
    13441 aagagactta tcgccaggcg ctggagaacg tctccacctg ggtacgtgct tactacgata
    13501 ctgatgatgc caccaccaaa gcgttcctcg acgaggtgga ccagttaagc cagcaaaata
    13561 tctcgatgga tcttccggaa accctgcaaa gccaggcgat gctggaaaaa ctgatgcaga
    13621 ctcgcgtgcg taacctgctg gcacaaccgg cagcagggac aacggaagct aaacctgcac
    13681 ctgcacctgc acctgcacct gcacctgcac ctgcacctgc acctgcacct gcaccgcaag
    13741 gtgatactcc ggcagccgcg ccgcaaggag aataaccatg ctaaaagtgt tattactctt
    13801 tgttttgctg attgcgggga tagtggttgg cccgatgatt gccggtcatc agggttatgt
    13861 gctgatccag accgacaact acaatatcga aaccagcgtc acgggcctgg cgatcatatt
    13921 gattctggcg atggtagtgc tgtttgccat tgagtggcta ctgcggcgga tcttccgcac
    13981 tggcgcgcac acccgtgggt ggtttgtcgg gcgtaagcgt cgccgtgcac gtaagcagac
    14041 cgaacaggcg ctgctgaaac tggcggaagg cgattatcag caagttgaaa agctgatggc
    14101 gaaaaatgcc gatcacgctg aacaaccggt ggtgaactat ctactggctg ccgaagccgc
    14161 gcaacaacgt ggtgatgaag cgcgcgccaa ccaacatctg gaacgcgcag cggagctggc
    14221 cggcaatgac accattccgg tagaaatcac ccgtgtacgt ctgcaactgg cccgtaatga
    14281 aaaccatgct gcacgccacg gcgtggataa gctgctggaa gttacgccac gccatccgga
    14341 agtattacgt ctggcggaac aggcgtatat ccgcacaggt gcatggagtt ctctgctgga
    14401 tatcatccca tcaatggcga aagcccatgt tggtgatgaa gaacatcgtg caatgctgga
    14461 acaacaggca tggattggcc tgatggatca ggcgcgtgcc gataacggta gtgaaggttt
    14521 gcgtaactgg tggaaaaacc aaagccggaa aacgcgtcat caagtagcgt tgcaggtggc
    14581 aatggcggaa catcttattg aatgtgacga tcatgatact gcccagcaaa ttatcatcga
    14641 tggcctgaaa cgccagtacg acgatcgcct actgctgccg attcctcgac tgaaaaccaa
    14701 caatccggaa cagctggaaa aagtgctgcg ccagcagatc aaaaacgtcg gcgatcgccc
    14761 gctgttgtgg agcacactgg gccagtcact gatgaagcac ggagaatggc aggaagcatc
    14821 gctcgccttc cgcgcagcgc tgaaacaacg tccggacgcc tacgattacg catggcttgc
    14881 cgacgcgctg gacagactgc acaagccgga agaagctgca gcgatgcgtc gcgacggttt
    14941 gatgttaacg ttgcagaaca acccaccaca gtagttcctt ctcacccgga ggcaagcacc
    15001 tccgggacct ttctgatgca taaaaaaacg cctgctctta ttacggagca ggcgttaaaa
    15061 caggtctgta tgacaacaag tgggtgcttc actcaacgtt gtgtccatgg tgtctgatga
    15121 ggcataagcg acatctgtca gtggacgata agcaccgtaa acggctctgc gtcattccgg
    15181 agtttatgag gcactaaggc gaacataaga gatggaatga gcatctactc gtttattatg
    15241 ccacagagaa tcgggaaata acatccctta acacttgtta tgagataatt ctgtaatcct
    15301 ctttgcttcc tgagtaataa cttcctgagt gaatatttaa cctgagcttg atcctacaca
    15361 tacttattat gaatgataaa attcattcaa ttaataacac atatattaat tgccgttaaa
    15421 actaaaaaaa gcatcaataa tcaacgcgat ataataaacc tgccttacat atcaactgcg
    15481 ccagaggtag gattgaaaac gctctcctga ttttccaatt cattttctgg ataaataaat
    15541 aatttatttt tgtcactatt atttatgtaa tcatcctgtc agggagaggg atctcaatta
    15601 tcaatgctta attacgtcat cattttgatg acatgaaacc ggagcatttt gcatggaatt
    15661 ttcgttttca cccaaacttc ttgttgttgc tgtcgccgcc gctcttcctc tcatggccag
    15721 cgcagcagat accccgtcaa ctgccaccgc acgcaaaggc tttgccggat acgatcaccc
    15781 aaaccagtat ccacagaacg ctgcaattta ttgaatttgc acgatcatgt aggccggata
    15841 aggcgtttac gccgcatccg gcaatcaacc gcaggcggcc acaaaatggc cgctatttta
    15901 cttattcacc accagcaaat gtgcctgcat aatgtcactg gccgggcgac cgtgcgccag
    15961 caaatcagcc attgctttaa gatatggcgg tagatggcgg aaataacgct gatacccggc
    16021 acacaaataa ttcagccccg gtttgccgct ggcatcgagc atgaagcggt gtttcgggca
    16081 gcctccccag cacgctttta acacgttaca actgcgacac tgcgccggta attgcttaaa
    16141 tttatcttca ccaaacacct gctgttgcgg ggaatcgatc atttctgcaa ttgtttgctg
    16201 atgcatattc cccagccgat attgcggata aacatagtga tcgcaggcgt aaacgtcgcc
    16261 gttgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa ccgcacccgg
    16321 cgcaccgaca aaattggcaa acgcccattc gatattcatc acgaaaatct tgccgacgtc
    16381 gcgtttgatc cagtggtcaa aaatcgccac cagaaattca ccaaattcat cggggcgcac
    16441 agaccattcc gtcagttccc cctgaatatc accaggcgca tgtagtttca gtccatcgct
    16501 ggcagctgtt tcatcagcta ggcgctcgac caccggaata aactggatga attcgactcc
    16561 cgcatcgcac aaaaaatcat aaacctgcaa cggttgctgc gcgctggtgc ggttgacgca
    16621 gaccagcacg ttatagtcga catgatgttt ttgcagcagc gtcagggcac gcatcaccag
    16681 cttatgcgtt ggtctgccac ctttggtcac gcgatattga ttgtggatct cagccggacc
    16741 gtccagcgat aacccaacaa gaaaatgatt ttctgccaga aatgcacacc atttatcatc
    16801 gagcagcacg ccgttagtct ggaagctgtt acttatcttc ctgccagcac cgtatttcgc
    16861 ctgtaacttt acggcacggc ggtaaaaatc cagcccgagt aacgttggtt cgccgccctg
    16921 ccaggtaaaa gccacttcgt tttgcggttc gctggcagcg atatagtgac ggacatacgc
    16981 ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg attgtttttc
    17041 gagataaaaa cagtagtcac agttcagatt acaatcggaa ccactcggtt tcgccatcac
    17101 atgaaaagca cgcgttggaa cctgttgcag catggtcact ccttgcctga agtcatcctt
    17161 caatacgtta agggcgaaat cataccgtgg ggcaatgagt tattacgggc gtttattgcg
    17221 aagagagggt gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg gttcaaaatt
    17281 ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt gcgctaccaa
    17341 gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattatt taaagtcgtg
    17401 gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct gaagctagcg
    17461 cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg tggctaatgg
    17521 gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga gctatagcca
    17581 ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc gggactaaat
    17641 ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc gctctatcca
    17701 gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt cccacccctc
    17761 tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgt tttgctgctt
    17821 attcctccag tttatgcgct ttcccgtggc gattaaggcc aaaaacttta taaatcgccg
    17881 tcaccgccag cataaagatg atgccaacaa acagcgacat acgcgtatct tcattaaagt
    17941 acatgccgat caaaacgcaa atcaggaatg ccattgttac gtagttggcc cacgggaaca
    18001 ggattgagcg gaacggatgg ctggcaatcg ccgctttatg tgcacgccga aaacgcagct
    18061 ggcttatcaa tatcacaaac catggcacca tccccggaag cacgctggca ctgtagacgt
    18121 agacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca attagcagaa
    18181 tagcaataga tactgccaca cccgcaactg gtacgccgtg acgggaaact ttcgccatcg
    18241 ccgccggtaa ctgacggttt ttcgccagtg cgtagagcat acgtccgcaa ctgtacatgc
    18301 cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg cccgccgctg
    18361 cggtaatacc gattttggca aaagtcagta cgaacgggct gccgttgctg ccactttcat
    18421 tccacgggaa gatggtgacg ataacgaaaa tcgcgcctac gtagaaaatc aggatccgcc
    18481 acagcacttt gcctacggca ctgcgcaacg tcacctgcgg attcttcgct tcaccggcag
    18541 taatgccaat cagctccacg ccctggtagg acgccaccac aatacacagc gcggtcagga
    18601 accctttcca gccacccgca aagaaaccgc catgctctgt gagattgcta aaaccaatcg
    18661 actgcccgcc attgccaaag ccaaagaaaa tcacgcccag gccaatgaca atcatcacga
    18721 taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac aaccgcaccg
    18781 ccgccagatt cgccaacgcc accagcgcca ctgcgatcaa tgcgggtatc cactgcgcca
    18841 tctccgggaa ccagaactgg acataaacgc caacggcggt gatttctgag atccccaccg
    18901 ccatccacat aaaccaataa gaccaggcgg tgagataacc aaagaacggg ctcatataac
    18961 gatgcgcata aacggcgaac gaaccggtaa ccggttcgag gaacaacatt tcgcccattg
    19021 aacgcatgat gaaaaagacg aatagcccgg cgatgatata ggccaacaat acggatggcc
    19081 cggcccattt caaggtactg gcggccccca taaacaggcc gacgccaatg gtgcccccca
    19141 gggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt ttgttatctg
    19201 ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc gttatgggta
    19261 catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg gcaaataaag cattaaaaat
    19321 ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga gtgaccactc cgtcgcttta
    19381 caaagagagg aaaatcagag gttgccggtg tagtgccagc gtaaataacg cagcaaacga
    19441 agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc cagtcccagc
    19501 gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta agtcccgcca
    19561 acgcccatat acagcgcatc tggatgcacc agacggcagt cgcgcatgaa gatctcctgc
    19621 tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc tggcatgaat gcgttcaaac
    19681 agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat attcacattc
    19741 cactggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc gcccacaaga
    19801 aataccggcg tcccttcttt gcctgcgcgc gccatcagct cttcccagag atcggcaccg
    19861 gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac aacgctgatg
    19921 ccatccgcat atttaaattc ggcagcgtta attaactccc tgacctcggc gttatcttca
    19981 atggtcagca ttttttcagc attaatggca accagcgttc cctgcttaag ctgcccgtca
    20041 gcaaacagat aatcgagggc gtgctgcata tcacgccaac caatcaactg taagccacgc
    20101 agcgtatagg tcggtgccgt ggtgttgtta ttcattgtta tccttcaacc tgcgtccgga
    20161 gcgatgattt tgtacgttta tgaatgagtc cggcgctttc aaaaagccag tacaacagtt
    20221 ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa gactacgcgt gagacaaacg
    20281 aatccagccc ttcacgtgcc agcacgatca tattgaagat cgccccaaag cagaaactgt
    20341 gcaatatcgc agccttatag cgattaggct cgcggttgcc cagctcatac agccagtcga
    20401 accatttgat gatcagacca accacgatcg ccccgagcgg gatgaacaac gcgccgccca
    20461 tcaccaccag tgagcctata agcgtaggcg agatcgccag tccggagtgg ttattcagca
    20521 cttccctggt aaagtagttg gctgagttca gcaccatact cgggcgaccc ggccacagcc
    20581 aggaagggat aaagacatag aaatcgcgga caattggagc caggccctgg aagtcgatgt
    20641 tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg cgagaaggtg tcgcgagtga
    20701 gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccatag cgttttagcg
    20761 ccagccagaa catgcccaca atccccagca cgcccgccgc cgccagcatc cacaacgaaa
    20821 tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg atattggcgc
    20881 gagtgccgcc gacaatcata taagtcagca agccaaaggc gaccgtgctg acgaggaaaa
    20941 acagccacgc tttgctatcc tggcgcagaa agtagaccac cagcatcgcc gggatgaaaa
    21001 agtaaaagaa gcgttttaac gccacgccgg agacttcact ggagaagatc tgactgtagg
    21061 agttaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg cttaccagcg
    21121 cgatgcccat caggatcacc cacgtaagat tggtctccac gcggttcatg gtaaacagcg
    21181 gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt tttgtaggtg acatagtaaa
    21241 ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacaa gatttctggc ggcgcgacac
    21301 caacatcaaa gcgaaatacc agcacgctgg tcagcgggaa gccgaagaaa aaggtgagca
    21361 aaaacagcaa tgaaaagaag acattgaagt taaagcgcac acggcgaaac tcaaaccaag
    21421 tcagcgtggc aataaacagc gtgcagagca gccagaccac aaacaggcca ctgaattgca
    21481 gcagactcat gcgacctccc cggcggcaat cgccaacacc cgctgccagc cttgtagata
    21541 gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc gctgcgcttc
    21601 acgcacaata tcctcgttga gatcgtcggt agtaaacagc accggcaaat gctgttccgt
    21661 catatcctgc cagaacggat tttcccggtt aagcacacaa ggaatgcccg cctgaatcag
    21721 taagcaaagc gtaccaatgc cctgctggcg ggcaaaaata aagtaaccaa gatcgcactg
    21781 acgaagtagc gccagatagg cgtcaaattc cagtttttcg ctcaaaattt gcagattttc
    21841 ttcgctgaat aactccagcc ccgcctgacg aacttcctca atgtacgctt cgttattagg
    21901 cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat gaacggcgcg
    21961 caaggcagca atatggtcat tgctgcggtc accggagttc cccaccagaa tggtcatttt
    22021 cccttcacgt tgccgatcgt tcgccatcgt attgagagaa gggtccatcc gcgtcgggaa
    22081 gtacagcagt tcgccccgca cctttggatg cgttttggca aaaaagctca aatcaccgcg
    22141 ggtggcaaat acacagccga ctcgcttttg cgccaggcga cgtagcgggt aaaaaagctt
    22201 atatctcaag ccactggaaa gctcatacag gtctgccccc cagatatgcc agaaaaactg
    22261 gctgggctta atgccaccac tcagcagagc cagccacagt gtgggattga actgaccgtg
    22321 gaaaaaaaat cgctgctgac ggttagcttt tgctttcgcg atgaccgctt ccgccagcga
    22381 ttttttccca gggaaaaatt gcacagaaag cgccggacag ctatcactta agccgtcgtc
    22441 cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc gcggccagcg cgtcattgaa
    22501 aaaccgcaaa acggttcggt tatggtgagg gatatccgat cccagtacgt gaatcagtac
    22561 agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa atagacgata
    22621 taagttgcca tatatgcctg cgccgcgccc agtgcaccat gcgcagggat tagccagtgg
    22681 gcaaatacca tcaataaagt gaactggctg acttccgcca gaatataaaa ccgcagtgac
    22741 gctttggcga tcaccagata accaaagaca taagcgccca cttttagcac atcacccact
    22801 aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag caaccagata
    22861 gcaaaatcac gcagcaacca gacggtaaaa ctcgccgccg ccactgccgg taagacgaat
    22921 tttagcgatt taaccacttc ccgggtgata tcgcgctttt ccgttagccg cgacaacgtg
    22981 ggcagcaaat atacgctgaa cgatgccgta atgaattgca ggtaggcatc ggaaatactg
    23041 ctcacccctt gccagatccc cacttcatcc cagctatact gcgccgccag cagtttacgc
    23101 atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgccatgag cgtaaatttg
    23161 ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa cgggatgaca
    23221 ccacgtttga tcaacatgat ggcggcagga attaccacca gcgcgggaat cagcgccaga
    23281 cccagcaacg ccccttcata accgcccaaa cggtaactga cgtagtacgc gagaacgcca
    23341 atcaagctgc cgacaatcag cgataacgca ttacctgcgg catcgcgaaa gcctttcatc
    23401 agcgccagta acaggttgcc ccaggcgatc cccatttgca ccagcgccac taaacgcacc
    23461 agcccctgat agtcggtatt accaaacaat ccctggctga ttggcgcagc tgccagcaca
    23521 aaaaccagcg ccatcagcgt agagaaacca agtaccatcg ctgatgaagt gccgaccacg
    23581 cggcgcagct gttgcggatt atcatggtac tgggcaacgt atttggttac accgttaaag
    23641 atgccagccc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt tgccgccagc
    23701 ccaagccccg ccggaccaaa tgacaccgcc agcaacttac cgaccagtaa cccggcacca
    23761 atcttgacca gtgtactggc cgccgtccac aaggacgctt ttgccaacga catatcagga
    23821 gaagtagttc aacaaagtcg caattaccgt acgctgattg acgggcgaca ggttgtagaa
    23881 cagcggcagg cgcagcaggc gctcgctctc tttggtggtg tagcgatctt caccgtggaa
    23941 ctcaccaaag tgttcccccg cagggcaacc gtgcagcgga atgtaatgaa acaccgccat
    24001 gatttccgct tctttcagaa agttaatcaa cgcgctccgg tcatcaatat cccgcagttt
    24061 aatgtagaac atatgcgcgt tctgcacgca gccatcggga atcgacggca gctcgatacg
    24121 cccggctttc gccagaggcg ctaacgcatc gtagtaattt tgccacagcg ccagacgttg
    24181 ctggttgata cgatccgctg cttccagttg cgcccacagg tatgcagctt gcagatcgga
    24241 catcaaatag ctggagccaa tatcgcgcca ggtatattta tcgacctgac cacggaagaa
    24301 ctggctgcgg ttagtgccct tttcacggat gatctcggct cgttcgatta acgctttatc
    24361 gttaatcagc gtcgcgccgc cttcaccacc cgccgtgtag tttttggttt catggaagct
    24421 aaagcagcca atatgaccaa tggttcccag tgcacgccct ttgtaagtgg acatcacgcc
    24481 ctgagcggca tcttccacca caaacaaatt atgctttttc gccaacgcca taatggtgtc
    24541 catttcgcag gccacacccg cgtaatggac cggcacgata acgcgcgttt tgtcggtgat
    24601 cgctgcttca atcaacgttt cgtcgatgtt catggtgtcc gggcgaacat ccacaaagac
    24661 gattttcgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt agctcggcat
    24721 gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca gcgaagcggt
    24781 gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt tgctccagcc actgctggca
    24841 gcgacgggta aaaccgccat cgccacacag tttgccgcta cccattgccg actgcatata
    24901 gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat cacctgtata
    24961 accagtacgc ggtgctttct acattcgcac cactttgtat gtatcgttta agcgcggcgg
    25021 tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc gcgagcatac gcccagttta
    25081 gcgccgtttg catcagctca gcacctgcac cgcgtccagc cagcaggcca attcgcgcat
    25141 ctgtcgcatt gagttcccgt aaagagacat agccgcgaat atcgccggac gccgcacgta
    25201 gaatcagaca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac tgtgcataaa
    25261 agcgaccgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc gcaaatgcgg
    25321 cgctagctaa ctgacgtaat gcgggaatat cggtctcttg tgccacgaca gcaccgctat
    25381 cactgacatt gttcacgggt agcgccaaat caacttcacc ttctaccagg gagaatccca
    25441 gctgttgcag ggcatccagt tcacccgtat ttgatgccgc aattttggcc tgcacccgtg
    25501 accacggcgc taacgcgtct ggcgtcagga gcggtgcttc agacgtaatg cgcacgatgg
    25561 cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg gcgcggacgg
    25621 gcacgaagta actccagcag atattggccg tagccagttt tcgctaatga actggcagca
    25681 cgcttcacac cctcgtcatc gagccagccg ttacgccagg cgatctcttc caggcaggca
    25741 atcttaaagc cctggcgttt ttctactgtc tgcacaaagg tactggcttc aatcaggctg
    25801 tcgtgagtgc cagtgtccag ccaggcaaat ccgcgcccga gcagttcaac ggtcaggttg
    25861 cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg ctccgacggc
    25921 ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc ggtcaccgcc
    25981 cagtttgact tcggctgttt tggcttttct tccagcgaga tagcgcggaa attgtcgtca
    26041 aactccacca cgccaaagcg ttcctggtcc atcacctgat agccaaatac cgtcgcccct
    26101 tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgaccgaa gaagatgtta
    26161 tcgcccagca ccagacaaga aggttcaccg ttgaggaagg tttcaccgat gataaaggcc
    26221 tgcaccagac cgtccgggct gggctgttcg gcgtattcca gctggatacc gaactcacta
    26281 ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat gatgagaatt
    26341 tcgcggatac cggccaacat cagcaccgac agcggatagt aaatcattgg cttatcgtaa
    26401 atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt gccggaaccg
    26461 cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt cagacctaaa
    26521 cgctcgccct gatagctgcc gtcctgcacc tgcttccacc agctttcatt agccagatac
    26581 cactgcaccg ttttacgcat tccactttca aaggtttcct gtggcagcca gccaagttca
    26641 cgggcaattt tcgaagcatc aatggcataa cgcagatcat gccccggacg gtcagcgaca
    26701 aaggtgatca agtcacgata gtgcaccacg ccgtgcggct tgttcggagc cagttcttcc
    26761 agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg accaccaata
    26821 ttataggttt caccgacttt cccggtggtc gccacgcaat acagcgcgcg ggcgtgatct
    26881 tccacataca gccagtcacg gatttgctgc ccgttgccat ataccggcag cggtttaccc
    26941 gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta aggaccgtag
    27001 ttattcgagc agttggtgat aagcgtcggc agaccgtagg tccgcagcca ggcgcgcacc
    27061 agatggtcgc tgctggcttt tgacgcggaa taagggctgc tcggcgcata cggcgtggtt
    27121 tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcggt ggagatatga
    27181 tgaaaacgga acgctgattt tttatcttcc gtcagcgtat tccagtaagc ccgcgccgct
    27241 tcaagcaatg tataagtccc gacaatgttg gtttcaataa acgctgccgg gccgtcaata
    27301 gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg atgctcagtg
    27361 aatacgcgtg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc aaagcgatcg
    27421 ctttgcgcga ccggtgccag cgacatcagg tttccggcgt aggtcagctt atcgaccact
    27481 accaccgcgt cgctcgtttc gttgatgata taacgcacca gcgccgagcc aataaacccg
    27541 gcaccacctg ttatcagaat ttttctcatc agcgccagac tcctttggca tcgacgacat
    27601 actgctgatg gacattgtcg ccattgataa ctttaaactg actatgatcg accagcatca
    27661 ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc cagagtacaa agcccggtca
    27721 gttttttcgg caactggtgg atgttaggct caacaaccag agtttcgccg ctatgccact
    27781 gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg caggtcatca atattcggtt
    27841 taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg gtagccgcca
    27901 ggcaatcagc caccgccgct ttcacctgat cgataaccca gaacggtttg tgatcgttca
    27961 cttcgcgcgc ggtacggata agccgcgcct gctgggggtt ctgtgccacg ataaaccacg
    28021 gatcaacagc aatgcagtga ccgcccacgc cagggccagg ctgaagaata ttaacgcgag
    28081 ggtgacgatt cgccaggcga atcagttccc agacgttaat cccctgatcg gcacaaatca
    28141 gcgacaattc attagcaaaa gcgatattca catcgcggaa gctgttttcg gtgagcttac
    28201 acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa attttgtaca
    28261 gttcgctggc gcgggtcgaa caaaccggcg tcataccacc aatcacgcga tcgtttttaa
    28321 tcagctcgac cataacctgg cccggtaaca cgcgttccgg gcagtaagca atgttgacgt
    28381 ccgcctgctc gcccacctgc tgtgggaaag tgagatccgg acgcatctct gctaaccatt
    28441 ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc agcgcgcctt
    28501 ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata tctggctcat
    28561 gatcgccttt aaacggcgtg ggtacagcaa tcagccaggc atccgcttca actggcgtcg
    28621 tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacgctcgcc aaatcaggct
    28681 cgacgatatg gatttcgcca cgattgatgg tatcaaccgc gtgttggttg atatcgacac
    28741 caatcacctg tttttgccgt gaggcaaacg ctgctgctgt aggcagcccg atgtaaccca
    28801 gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt ttaacgcttc
    28861 cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc tcatagtttg
    28921 atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct gcttatccgt
    28981 gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga cgctcagtgg tatcgcgcat
    29041 caccagcacc ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg agtcggtcaa
    29101 aatcagccag gcgtggttca tcaaccagac aaacggtaaa tactcctggg gatcgatcag
    29161 aatgacattt ttcacatgcc ccagaatgcg attgaccggc tctctgacgt tcgggttgag
    29221 atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg gcaatgtctg ccaacgcgtg
    29281 gcagatttct tcaaaaccac gaccgaaact ctcacgcctg tgaccggtca ccagaatcat
    29341 ctttttattg gggtcgataa acgggtaatt tgccaccagt tctgaacgca gcgtgtcgct
    29401 gctcatcacc tgatcacgca cccataacag tgcatcaatg actgtattac cagtaatgaa
    29461 gattcggcta tccgcaacgt tttcacgcag caagttttgc cgggaagttt cggttggaga
    29521 gaagtgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg gccacggcga
    29581 atagagatcg cccgtgcgta aaccagcctc aacgtgacca acaggaatac gctgataaaa
    29641 cgccgccagg ctggttgcca gcgtcgttgt tgtgtcgccg tgaaccagca cgacgtctgg
    29701 tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta tctctgtcag
    29761 gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa agagtttcag
    29821 cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct caaaaaaagg
    29881 agcttttgcc aacgcatgca ccaacggagc catcttgatg gcttccgggc gcgtaccaaa
    29941 tacagtcagt actttcacat cgattctctt cgaataagcg gcgagcgcct ttgcgctcgc
    30001 cgcagcagtg ttgctatttc gagcaacggc gggttaatgc gacaccagcc ccgatcagcc
    30061 ccccgacaat gccccacata atcatcagga aggcacgacg tgggctatcg cgttttaccg
    30121 gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg cggatccagg gttggaccaa
    30181 cattcagggt gtttaacatg gcccgattct gatcatagtc gagatcaaag gccggaccga
    30241 cggcctgtaa attttccagt cgagcctgga gcattggacg cccaagcagg aacatttctg
    30301 aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg cgaaatatta tgctgctcag
    30361 caattttcag cgcctgctca atgctgttca tccggcggtc gtagatggct ttcgccacct
    30421 cttcctgacg cttcacctga gctttcatct ggatggtgcg tgccgcccat gcgcctttca
    30481 gctcatcatt cagatggctg gctgcacgct ggctggcaaa agcaacatac tgacgtaaca
    30541 ggttattagc gtcaggcgcg gtttcggcaa taagcttcac gctgtcattg accgcgcggg
    30601 taaagtctcc ggggataaac tggatgttgt taatcatttc atccagcaac gccgcatcgg
    30661 ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt ttgcagccag aactctctgc
    30721 gggtatccca cgaggccagt tgcataacaa actctttata ggcttcgtcc atgaccgatg
    30781 gttgatcggc agaagccatg tttgaacgga catccaggtt acgcaaaaat tgctgctgcg
    30841 agtaatatcc ccccagcata ttcaccgttg gacgatcggt aatcgccgtc gagctccact
    30901 cctgacgagc aaaaaaagta tacgccagcg cgattaacgc aaacgccagc cccatgccaa
    30961 taatccatag cttcccagcc cacaaggtac gaaacaaccc acgaatatcc agttcatttt
    31021 cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat cacatcctca tttatttggt
    31081 taaattgggg ctgccaccac gatttctacg cagtctgcgt tttacgcgct taataaagcg
    31141 agcaactttc caggcacgct taatgcaata tccatagagg aagaatgcta gcaaaaagag
    31201 caccagcatg acccactccg ggacaaaatg agaatattct gccagcacgc caatggaagc
    31261 gagcagtgct gcggcaaggg taatcagcac aaacgcctga cgggaagtaa acccggcacg
    31321 catgatcaaa tggtgaatat gctgacggtc aggagagaat gggctcatgc ctttacgcag
    31381 gcgacggtac ataatcgcca ccatatccat tagcggaatg gcgattatcc acaaagcggt
    31441 aaccgggctg atgggatggg ttttgccctg ggtcgtttcg agcaggatcc agataacggt
    31501 aaaaccaatc agcgtactgc ccgcatcacc cataaagact ttgtagcggc gacccaggat
    31561 accaaggtta agcatgatgt atggcaggat ggcggcgatc atcgcaaagc accagattgc
    31621 gaggctggtt tgcccgtcga accacaaaat cataccgatt gctgcaaacg agacgcagga
    31681 caacccgccc agcaagccat caatgccatc aaccatgttg aacgcattaa tggccgccca
    31741 gacggcaaat agcgtcagga agtaaccaaa cggtccgagc accatctccc aggagccaaa
    31801 gatataaccc aggctactga gataaagttt gccgaacacc atcataacaa tgccaacagc
    31861 ggcctgtatg gtggcacgga tttttacgct gatatcaaaa cggtcatcca gcgcgccaat
    31921 gaaaacaagc acaccggcac aagcgagata gagagatgca tgcggaatat agtaatcgac
    31981 aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc cccccaacga gaggtatcaa
    32041 tccctggtga cgtttgcgga agtttggttt atccactaaa ccgacttttt ttgccacctt
    32101 acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata ctgatgagat cagtactcac
    32161 tgtcagtaaa ttcacaatgc atgctctcag agaaaattat tagcagaagt ataaccacga
    32221 agacctttat tcagaaggga aagtccgttc cgaacccacc agtcctgtgc aaaaattaac
    32281 catttgatat gcaaattgca taacatgcca gcaaattcca ggaattttcc taacatcgcc
    32341 agcgcggcat taagattaca gatataaaag caaaacgcca cgtaaacacg tggcgttttt
    32401 ggcataagac aaatttatga gcgtttcatc atttcgaaga aatcgtcatt ggtcttggtc
    32461 atggccagtt tattaatgag gaattccatt gcatcgattt cgcccatcgg gtgaatgatt
    32521 ttgcgcagga tccacatttt ctgcagttct tcctgagtcg tgagcagctc ttctttacgg
    32581 gtaccggaac ggttgtagtc gatagccggg aagacgcgtt tttcagcgat cttacgagag
    32641 aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt aaataacttc gtccatttta
    32701 gaaccggtat cgataagcgc cgtcgcgata atggtcaggc tgccgccctc ttccacgtta
    32761 cgcgccgcac cgaagaagcg tttcggacga tgaagggcat tggcgtccac accaccggtc
    32821 aacactttac ctgacgccgg aacaacagtg ttgtaagcgc gcgccagacg agtgatggag
    32881 tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc gtttggcctt ctcgatcacc
    32941 atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa aggtagaagc aacaacttca
    33001 ccttttacca gacgctgcat ctcggttact tcttccggac gttcgtcgat cagcagaacc
    33061 atcagcacac aatccgggtg gttgtaagca atgctctgag caatgttctg cagcagcata
    33121 gttttaccgg ctttcggcgg tgccacaatc agaccacgct gaccacgacc gataggtgat
    33181 gccagatcca gtacgcgagc ggttaaatct tccgtagaac cgttaccacg ttccatacgc
    33241 agacgagagt ttgcgtgcag cggggttaag ttctcaaaga ggattttgtt gcgggcgttt
    33301 tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg caaaatagcg ttcaccttct
    33361 ttcggcgggc gaatcttacc agagatggta tcaccagtgc ggaggttgaa acggcggatt
    33421 tggctagggg aaacgtagat gtcatcagga ccggcaaggt aggagctgtc tgcggaacgg
    33481 aggaaaccaa atccatcctg caatatctcc agtacgccat caccaaagat atcttcgcca
    33541 ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct gcttacgcat acgagccagg
    33601 ttttccagcc ccatattttc gccgagagtg atcagctcag aaaccggcgt attctttaat
    33661 tcggtaagat tcatagtggt gtgagttctt aaacttgggg taaatctcga acttaatgtt
    33721 gtgaatggta tggcaggatc atccatgcct gtttacggtc atcgactcat gtcttttcgc
    33781 tgcctggtca caggagagta cgcagaactg aaacgacaag acggattgag tgacgagcca
    33841 gaaatctgtt cacttcgcat ttaagataaa acgggaagcg ttggaaatta caagattcaa
    33901 acttaataag gtatgtttaa tacgaagtca acactaagtt agcatgactc acgccgggcg
    33961 tccagttttt agcgacgggg cacccgaaca tgaaattccc ttacgccagg ttagcgtcga
    34021 ggaactcttt caactgacct ttagacagtg cacccacttt ggttgccgcc acttcaccgt
    34081 ttttgaacag cagcagagtc gggataccac ggatgccata tttcggcgca gtgccagggt
    34141 tttgatcgat gttcagtttt gcaacggtca gtttgccctg atattcgtca gcgatttcat
    34201 ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc tgcccagaaa tcgacgagga
    34261 tcgccccgtc cgctttgagt acatccgtgt caaaactgtc gtcagtcagg tgaataattt
    34321 tatcgctcat atataactcc acaggaataa gcctggcgtg ttggtttcgt tgttggtgta
    34381 acattaacca actaaaggtt gactttattt caccggatac gctttcgtaa agcaatagta
    34441 agctgatatt ctaccacact atgagcaaaa cacatttaac agaacagaag ttttccgact
    34501 tcgccctgca tccgaaggtt gtagaaatcc ttgaaaaaaa agggtttcat aactgtacgc
    34561 ccattcaggc actggccctt ccgctgacgc tggcgggtcg tgacgtagcc gggcaggcgc
    34621 aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac gtttcattat cttctctctc
    34681 atcctgcgat tgccgatcgc aaggtgaatc aaccacgtgc cttaattatg gcaccgacgc
    34741 gtgaacttgc cgtgcagatc catgccgacg cagaaccgct ggcggaagct accggcctga
    34801 agctgggtct ggcttacggt ggtgatggct acgacaaaca gctgaaagtg ctggaaagcg
    34861 gcgttgacat tctgattggc actacggggc gtttaattga ctacgccaag cagaaccaca
    34921 ttaacctcgg tgccattcag gtggtggtac tggacgaagc cgatcgcatg tacgatctgg
    34981 gctttattaa agatatccgc tggctgttcc gccgtatgcc gcctgcaaac cagcgcctca
    35041 acatgctgtt ctccgccacg ctttcgtacc gggtacgtga actggcgttc gagcagatga
    35101 acaatgccga atatattgaa gtggaaccgg aacagaaaac gggccaccgt attaaagaag
    35161 agcttttcta cccttctaac gaagaaaaaa tgcgtttgct gcaaacgctg atcgaagaag
    35221 agtggccaga cagagcgatt attttcgcca ataccaaaca ccgttgtgaa gagatctggg
    35281 gccacctggc ggcagatggt catcgtgtcg gtttgttgac aggcgatgtc gcgcagaaaa
    35341 aacgtctgcg tattcttgat gaatttaccc gtggcgatct ggatattctg gttgccaccg
    35401 acgttgccgc gcgtggtttg catattccgg cagtgacgca cgtctttaac tacgatttac
    35461 ccgatgactg tgaagattac gttcaccgta ttggtcgtac aggtcgcgca ggcgcaagcg
    35521 gtcactctat cagcctggcg tgtgaagagt atgcattgaa tttgcctgct attgagacct
    35581 atattggtca ctcaattccg gtaagcaaat acaatccgga cgcattgatg accgatctgc
    35641 caaaaccgct gcgcctcacg cgcccacgta caggcaatgg tccgcgtcgt actggcgctc
    35701 cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac tcaaaatcat tcaaactgta
    35761 tcaagatggc aagagaatga atcccgatga gcatactgta gtaagtgatt caggtgaaag
    35821 aatgcagcca acacagagac agattgaagg atgaagagta tgggttccac ctcgtcgctg
    35881 tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc tggttgtgcg cgaggtggct
    35941 ggaagcatcc agacgctgac gcgaataaaa cgcaaagtgc gtctggctgc tggcctgaat
    36001 agcgaaaatg ccctgtctaa tgaagcaatg gagcgcggtt ggcaatgtct gcgcctgttt
    36061 gctgaacgtc tgcaagatat ccctccctcg caaattcgcg ttgtcgctac ggcgacatta
    36121 cgtcttgccg tcaatgcggg tgattttatt gccaaagcgc aggaaatcct cggttgtccg
    36181 gtacaggtga tcagcggtga agaggaagca cgtctgattt atcagggcgt tgctcacaca
    36241 actggcggtg ccgatcagcg cctggtggtg gatataggcg gtgccagtac tgaactggta
    36301 accggcacgg gtgcacaaac cacctcgttg ttcagcctgt cgatgggctg cgtcacctgg
    36361 ctggaacgct attttgccga tcgtaatctg gggcaggaaa attttgatgc tgcagaaaaa
    36421 gcggcacgcg aagtgttacg tccggttgcc gatgaattac ggtatcacgg ctggaaagtg
    36481 tgcgttggtg cttccggcac cgtgcaggcg ttacaggaaa tcatgatggc acaggggatg
    36541 gatgaacgca ttaccctgga aaagttgcag caattgaaac agcgagccat tcattgcggt
    36601 cggctggagg aactggagat tgacgggctg acgctggaac gtgcgttagt gttcccgagt
    36661 ggtctggcga tcctgatcgc catttttacc gaactgaata ttcagtgtat gaccctggcg
    36721 ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc atctggccgt cgagcaggat
    36781 attcgcagcc gtacgctgcg taatattcag cgccgcttta tgatcgatat tgatcaggcg
    36841 cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg tggaaaagga atggcatctt
    36901 gaagcaataa gccgcgattt gctcatcagc gcctgtcagc ttcatgaaat cggcctgagc
    36961 gttgacttta aacaagcgcc gcaacacgct gcttatctgg tgcgtaatct ggatcttccc
    37021 ggttttaccc ccgcacagaa aaaattgctg gcgacgctac tgctcaacca gactaatccg
    37081 gtcgatctct catcgctgca tcagcaaaat gccgtaccgc cgcgcgtcgc agaacaactc
    37141 tgccgtttac tccgcctggc catcattttt gccagccgtc gccgtgacga tctcgtgcca
    37201 gagatgacat tacaggctaa ccatgaactg ttgaccttga cgcttccgca aggttggcta
    37261 acccaacatc cgctgggtaa agagattatt gctcaggaaa gccagtggca gagctatgtc
    37321 cactggccgc tggaagtgca ttaatgagta agtgccggat gcgatgctga cgcatctttt
    37381 ccggccttga ttatttccct cgttttgccg ccatcatcgc tttcagattc gccagatggc
    37441 tttgcccttt ctgcatccgt tcttcggcgc tgaccacttt gcgctcctgt tcccaaatca
    37501 gatcatcctg cggcagctcc agcaaaaagc ggctcggctc cgggcgcacc agctcgccgt
    37561 actgacggcg ttctttacac agcgtaaagg tcaattcctt ctgggcgcgg gtaatgccga
    37621 cataggccag ccgccgctcc tcatcgatat tatcttcatc gatgctgctc tggtgcggca
    37681 aaaacccttc ttccataccg accatgtaga cataaggaaa ctccagccct tttgacgcgt
    37741 gcagagtcat cagttgcacc tgatccagct cttcttcact ctcaccacgc tccatcatgt
    37801 cgcgcaaagt aaagcgcgtc accacctggg tgagcgtcat cggctcatcc agttcgctgc
    37861 cttccagcat ctccgtcatc cagctaaaca gttggttgac gttcttcatg cgcatttcgg
    37921 cggctttcgg gctgggcgat gtttcgtaca gccaggattc ataatccatg ccgtggatca
    37981 gatcgcgcac cgcggcaatc ggctcccgct ccgccagacg ctggatttct gccaaccagt
    38041 gagtaaagcg ggtcaatgct tcataaccac gtccgctaag cgtctgactc aggcccatat
    38101 caaagctggc ggtaaacatg cttttattgc gcgtcatcgc ccactcaccc agctttttca
    38161 gcgtcgccgg gccaatctct ctcttcggcg tgttaacgat acgcagaaat gcgctgtcat
    38221 cgtccgggtt agtcagcacg cgcagataag ccagcaagtc cttgatttca ggacgagaga
    38281 aaaacgacgt accaccagaa attttgtacg ggatgcggtt ttgcatcagg aatttttcaa
    38341 acactcgcga ctgatggtta ccgcgataaa gaatagcgta atatttgtac tgcgttttat
    38401 tgacgaagtg atgcgcaatc agttcaccag tgacgcgctc ggcttcatgt tcttcgttat
    38461 tcgcgcttaa aactttaagc tctgtgccgt aacccagttc ggagaacaga cgcttctcaa
    38521 agacgtgcgg gttattggcg atcaggatgt tcgccgcttt caaaatacgt ccggaagagc
    38581 ggtagttctg ctcaagctta atcaccttca gcgccggaaa atcctgactc agcagcacca
    38641 ggttttgtgg acgtgcgccg cgccaggagt agatcgactg gtcgtcatcg cccaccacgg
    38701 taaagcgcgc gcggctgccc accagcagtt tcaccagttc atactggctg gtgttggtgt
    38761 cctgatactc atccaccagc agatagcgaa ttttgttctg ccagcgctca cggacttctt
    38821 cattgcgttg cagcagcaac gtcggcaata aaatcagatc atcgaagtcg agaacattgc
    38881 ac
//
LOCUS       DAJTNN010000015        37259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTNN010000015 DAJTNN010000000
VERSION     DAJTNN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.015"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1644
                     /locus_tag="OO150_000722"
     CDS             <1..1644
                     /locus_tag="OO150_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCR5514965.1"
                     /translation="NVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVT
                     QHNTPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFE
                     HDINDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNK
                     ILTNQTNLTSTFYTGSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGG
                     LTRNGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAI
                     TCPAGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFAL
                     AQSGSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTY
                     SQYGKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAF
                     TLWSQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDF
                     QLNVYNLFDTDYVASINKSGYRYHPGKPRTFLLTANMHF"
     gene            1686..2363
                     /gene="ybiX"
                     /locus_tag="OO150_000723"
     CDS             1686..2363
                     /gene="ybiX"
                     /locus_tag="OO150_000723"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5514966.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            2437..2703
                     /gene="ybiI"
                     /locus_tag="OO150_000724"
     CDS             2437..2703
                     /gene="ybiI"
                     /locus_tag="OO150_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5514967.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            2968..3228
                     /locus_tag="OO150_000725"
     CDS             2968..3228
                     /locus_tag="OO150_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5514968.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLTEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3456..4541)
                     /gene="hcxB"
                     /locus_tag="OO150_000726"
     CDS             complement(3456..4541)
                     /gene="hcxB"
                     /locus_tag="OO150_000726"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5514969.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGYLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(4682..5644)
                     /gene="ybiB"
                     /locus_tag="OO150_000727"
     CDS             complement(4682..5644)
                     /gene="ybiB"
                     /locus_tag="OO150_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5514970.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(5672..7822)
                     /gene="dinG"
                     /locus_tag="OO150_000728"
     CDS             complement(5672..7822)
                     /gene="dinG"
                     /locus_tag="OO150_000728"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5514971.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            7942..8424
                     /gene="ybiA"
                     /locus_tag="OO150_000729"
     CDS             7942..8424
                     /gene="ybiA"
                     /locus_tag="OO150_000729"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5514972.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8656..10020)
                     /gene="rhlE"
                     /locus_tag="OO150_000730"
     CDS             complement(8656..10020)
                     /gene="rhlE"
                     /locus_tag="OO150_000730"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR5514973.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10249..10920
                     /gene="cecR"
                     /locus_tag="OO150_000731"
     CDS             10249..10920
                     /gene="cecR"
                     /locus_tag="OO150_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5514974.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10923..11918
                     /gene="hlyD"
                     /locus_tag="OO150_000732"
     CDS             10923..11918
                     /gene="hlyD"
                     /locus_tag="OO150_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5514975.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            11911..13647
                     /locus_tag="OO150_000733"
     CDS             11911..13647
                     /locus_tag="OO150_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5514976.1"
                     /translation="MNDAVITLNGLEKRFPGMGKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELASEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13640..14773
                     /locus_tag="OO150_000734"
     CDS             13640..14773
                     /locus_tag="OO150_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5514977.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            14784..15890
                     /locus_tag="OO150_000735"
     CDS             14784..15890
                     /locus_tag="OO150_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5514978.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIATNYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(15852..16262)
                     /locus_tag="OO150_000736"
     CDS             complement(15852..16262)
                     /locus_tag="OO150_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5514979.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16395..17146
                     /locus_tag="OO150_000737"
                     /pseudo
     CDS             16395..17146
                     /locus_tag="OO150_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            17143..18384
                     /gene="clsB"
                     /locus_tag="OO150_000738"
     CDS             17143..18384
                     /gene="clsB"
                     /locus_tag="OO150_000738"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5514980.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18384..19340
                     /locus_tag="OO150_000739"
                     /pseudo
     CDS             18384..19340
                     /locus_tag="OO150_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(19376..20089)
                     /locus_tag="OO150_000740"
     CDS             complement(19376..20089)
                     /locus_tag="OO150_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5514981.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLVIVLWPGIISHGRNKLLELAGKCHSEKLWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(20295..20999)
                     /locus_tag="OO150_000741"
     CDS             complement(20295..20999)
                     /locus_tag="OO150_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5514982.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(21136..21588)
                     /gene="moaE"
                     /locus_tag="OO150_000742"
     CDS             complement(21136..21588)
                     /gene="moaE"
                     /locus_tag="OO150_000742"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5514983.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFAGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(21590..21835)
                     /gene="moaD"
                     /locus_tag="OO150_000743"
     CDS             complement(21590..21835)
                     /gene="moaD"
                     /locus_tag="OO150_000743"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5514984.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(21828..22313)
                     /gene="moaC"
                     /locus_tag="OO150_000744"
     CDS             complement(21828..22313)
                     /gene="moaC"
                     /locus_tag="OO150_000744"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5514985.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22316..22828)
                     /gene="moaB"
                     /locus_tag="OO150_000745"
     CDS             complement(22316..22828)
                     /gene="moaB"
                     /locus_tag="OO150_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5514986.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(22850..23839)
                     /gene="moaA"
                     /locus_tag="OO150_000746"
     CDS             complement(22850..23839)
                     /gene="moaA"
                     /locus_tag="OO150_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5514987.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(23827..23971)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24236..25144
                     /gene="yvcK"
                     /locus_tag="OO150_000747"
     CDS             24236..25144
                     /gene="yvcK"
                     /locus_tag="OO150_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5514988.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(25336..27357)
                     /gene="uvrB"
                     /locus_tag="OO150_000748"
     CDS             complement(25336..27357)
                     /gene="uvrB"
                     /locus_tag="OO150_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5514989.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(27936..28613)
                     /gene="bioD"
                     /locus_tag="OO150_000749"
     CDS             complement(27936..28613)
                     /gene="bioD"
                     /locus_tag="OO150_000749"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5514990.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(28606..29361)
                     /gene="bioC"
                     /locus_tag="OO150_000750"
     CDS             complement(28606..29361)
                     /gene="bioC"
                     /locus_tag="OO150_000750"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5514991.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(29348..30502)
                     /gene="bioF"
                     /locus_tag="OO150_000751"
     CDS             complement(29348..30502)
                     /gene="bioF"
                     /locus_tag="OO150_000751"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5514992.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(30499..31539)
                     /gene="bioB"
                     /locus_tag="OO150_000752"
     CDS             complement(30499..31539)
                     /gene="bioB"
                     /locus_tag="OO150_000752"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5514993.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            31626..32915
                     /gene="bioA"
                     /locus_tag="OO150_000753"
     CDS             31626..32915
                     /gene="bioA"
                     /locus_tag="OO150_000753"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5514994.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            32974..33450
                     /locus_tag="OO150_000754"
     CDS             32974..33450
                     /locus_tag="OO150_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5514995.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            33602..34885
                     /locus_tag="OO150_000755"
     CDS             33602..34885
                     /locus_tag="OO150_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5514996.1"
                     /translation="MNTFSVSRLALALAFSVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGCQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35026..>37259)
                     /locus_tag="OO150_000756"
     CDS             complement(35026..>37259)
                     /locus_tag="OO150_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5514997.1"
                     /translation="LASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSSHNTSGNMDKL
                     KIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGL
                     SAAQRYGGIFVPPHIAIIHQYMREMMAGGGKMILGSDSHTRYGALGTMAVGEGGGELV
                     KQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPG
                     VSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDG
                     CISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAHGKAKLSLLDK
                     VENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAK
                     KGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREGSKPANGQMSA
                     VALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPL
                     IYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGLAEFTL
                     SRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYA
                     VKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGD
                     YIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKN
                     RQM"
BASE COUNT         8737 a        10078 c         9640 g         8804 t
ORIGIN      
        1 aatgtaatgg gcgaaaaaac gcacgatgcc ggacgcgaca aagtcaaaaa tgagcgttac
       61 ggcgtcgccc cttctgtcgc ttttggcctt ggtacagcga atcgtttgta tcttaattat
      121 ctgcatgtca cccagcacaa cacgccagac ggcggcattc cgaccatcgg tttgccgggc
      181 tattctgccc catctgcggg aacggcggcc ctgaatcatt ccggaaaagt tgatactcat
      241 aacttttacg gcacggattc cgattacgac gattcgacca ccgacaccgc caccatgcgt
      301 tttgagcacg acatcaacga taacaccacc attcgcaata ctacccgttg gtcgcgcgta
      361 aagcaggatt acctgatgac ggcgattatg ggcggggcgt cgaatattac tcagcccacc
      421 agcgatgtga atagctggac ctggtcacgc acggcgaata ccaaagatgt gagtaataaa
      481 attctcacca accagaccaa cctgacctcg acgttctata ccggttctat cggtcatgat
      541 gtcagtaccg gcgtggaatt tacccgtgaa acgcagacta actacggcgt taatccggtg
      601 acgttacccg cggtaaatat ttatcatcct gacagcagca ttcatcccgg cggcctgacg
      661 cgcaacggcg caaacgccaa tggtcagacg gataccttcg caatttacgc ctttgatacg
      721 ctgcaaatca cccgtgattt tgagctgaac ggcgggatcc gtctggataa ttatcatact
      781 gaatatgaca gtgccaccgc ctgcggcggc agcggacgcg gtgccatcac ctgcccagct
      841 ggtgtggcaa aaggttctcc ggtcaccacc gtcgacaccg ccaagtcggg caatctggtg
      901 aactggaaag ccggggcgct gtatcacctg acggaaaacg gtaatgtcta tattaactat
      961 gccgtttccc agcagcctcc gggcggcaac aacttcgccc ttgcgcagtc tggcagcggt
     1021 aacagtgcca accgcaccga ttttaaaccg caaaaagcca acaccagcga gattggcacc
     1081 aaatggcagg ttctggataa acgcctgttg ctcaccgccg cactgttccg tactgatatc
     1141 gaaaatgaag ttgagcaaaa tgatgacgga acttactcgc aatacggtaa gaaacgtgtc
     1201 gaaggctatg agatatccgt ggccgggaat atcactcccg cgtggcaggt gattggcggc
     1261 tatacccagc aaaaagcaac catcaaaaac ggcaaagatg ttgcccagga tggttcctca
     1321 tcgctgccgt ataccccgga gcacgccttc accttatgga gccagtatca ggcaaccgat
     1381 gatatctctg ttggcgcggg cgcacgctat atcggcagta tgcataaagg ttcagacggc
     1441 gcggtgggaa cgccagcgtt taccgaaggt tactgggtcg ccgatgccaa actggggtat
     1501 cgggttaatc gcaatctcga cttccagcta aacgtttaca acctgtttga taccgattac
     1561 gtcgcctcaa tcaacaagag cggctaccgt tatcacccgg gcaagccaag aaccttcttg
     1621 ctcacagcca atatgcattt ctgattcaga tgtggggcgc aggccccact ttttggagaa
     1681 attgtatgat gtaccacatt cccggcgtgt tatcgccaca ggacgtcgct cgttttcgcg
     1741 aacaactgga acaagccgaa tgggtggatg gacgcgtcac caccggcgca caaggtgcgc
     1801 aagttaagaa caatcaacag gtagacaccc gcagcacgtt atacgccgcc ctgcaaaatg
     1861 aggtgctgaa cgcggttaac caacatgctt tattctttgc cgcggccttg ccgcgtaccc
     1921 tttccacgcc gctgtttaat cgctatcaga acaatgaaac ctatggtttt catgtggatg
     1981 gcgcagtacg cagccatcca caaaacggct ggatgcgcac tgacctttct gccacgctgt
     2041 ttttaagcga tccacaaagc tacgacggcg gcgaactggt cgttaatgac accttcggac
     2101 aacatcgggt aaaactcccg gcaggtgatc tcgtgttgta tccctccagc agcctgcatt
     2161 gcgtgacacc cgtaacccgc ggcgtacgag tggcatcatt tatgtggatc cagtcgatga
     2221 tccgcgatga taaaaagcgc gccatgctgt ttgaactaga caaaaatatt cagtcgctga
     2281 aaagccgcta tggtgaaaat gaagagatcc tgtcgctgct caatctttat cataatctgc
     2341 tgcgggaatg gtcggagatc tgacgcttga actgcccagg ttcgtcccta tattagctac
     2401 acttaactgt acaagcattg atatggggag gacgatatgg catccggttg ggctaacgat
     2461 gacgccgtca acgaacagat caacagtaca attgaagatg cgattgcccg cgctcggggt
     2521 gaaattccgc gtggcgaaag cctgtatgaa tgtgaagagt gcggtgcccc catcccgcaa
     2581 gcccgtcggg aagccattcc tggcgtgcgc ttatgtattc attgtcagca ggagaaagat
     2641 ttacaaaaac cagcgtatac aggatataat cgcagaggtt cgaaagacag ccagttacgt
     2701 taactacaac tggcgagatg catagcgagt ataatttgta ttttgcgtgg cattccgtgc
     2761 ctttaacgct gctgtgactg ccacgcggca taaaacaagt ccataagtac aacaaataaa
     2821 tggtttatca gtatgttaga tgtcaatcaa tttatttgaa caaggcggtc aattctcttc
     2881 gattttatct ctcgaaaaaa aacgtgatac tcatcacatc gacgaaacaa cgtcacttat
     2941 acaaaaatca cctgcgagag attaattatg aaaactatca atactgttgt tgctgctatg
     3001 gctctttcaa ctctatcatt tggcgtgttc gcggcggaac cggtaacggc atcccaggca
     3061 cagaacatga ataaaatcgg cgtggtttct gccgatggcg catccaccct cgatgccctg
     3121 gaagcgaaac tgactgagaa agccgcggca gccggtgcca gtggatacag catcacttct
     3181 gccaccaaca acaataaatt aagcggtact gcggtaattt ataagtaatt cgtaggacgg
     3241 ataaggcgtt tacgccgcat ccggcaacag atgcctgatg cgacgctacc gcgtcttatc
     3301 aggcctacaa aatccgaacc gttggacgga tcaggcggtt acgctacgtc cagcacaagt
     3361 atttccctgt ctgtttgccg acagacgcat atgctctaac cctcattgat cctatattac
     3421 ccttgtttgc ccgtccgtta ctagacgggc tttttttagc tggctaactg ctgacagaaa
     3481 gcctgcaacg tctcttccga cataccaatc tgccgcgccg catcacaaat ggcctgccag
     3541 cttcccgcat ccaatggaat cccctgctcc tgccgttcgc gacgcgtgtt cacttcccac
     3601 tcgcccggta gcaaaatcgg cttatcgtca tcatgcggcg aggctttcac ccactcggca
     3661 aaggcttcag tctgcgcgct acaatccggc gcgccgaaca gttccgggtt gatgatgata
     3721 gtggtcatgc agttaagaat ggcatcggga ctggtttgta acgtttcctg atgcgtcgtt
     3781 ttaccgccag aaagcgcccc gccaagaatt tcacacatcg ctgcaagggc gtagccttta
     3841 tgttcggcaa aggtcaacag cgaccccaac ggcgactcct gcattaccgc cggattggtc
     3901 gtcggcacgc cgttaacgtc aatcaggcaa cctggcggca cggggacgcc tttatgccag
     3961 gcgacgcgag ttttaccaaa tgcgatggcg ctggtggcgt aatcaagcaa cagcgggaaa
     4021 ttatctttac gagggaaaac cacacagaac ggattggtgc caaagcggct gtcgcgaccg
     4081 tggaacggtg cgaccattgg aataccgacc acgctaacaa agtggataga gacaaacccc
     4141 gccgctgcac actgctccgc ccagtaaccg atacggccga tatgatgcga gttatgtagc
     4201 gccacggcag caataccgtg ctgatgcgct ttctcaatcc ccagcgccat cgcttcgtgt
     4261 gccgcgacct gaccgaatgc gcgatcgcca tcgagcgtga ctgccgcccc cgcctcttta
     4321 acgattttgg catgatggtt aatttgcaga tacccctgac tccaggaacg cacatagctt
     4381 gggatcatgc caataccatg agaatcatgc cctgccaggt ttgccgcgat taaatgatcg
     4441 gccactaatt tcgcttcttg ttcctcgcta cccatctgac gaaatacagc ctgaataaaa
     4501 ctgtgcagcg tctgagcatc aaagcgatga ccactttcca tggttaactc ctgtttatgt
     4561 tatgtgtttg ttgttttttt atgctgcacg ccgggcgcgg gaaggtcaat aaagatgcag
     4621 gaatttcata ttgcggtggg gtaaaagccg gatgacatgg ctcatccggc tgagaaaaga
     4681 attaaaatgc ctgattaacg cgcgccaggc cgtcgctgat agttgccgct tcacccgtag
     4741 cctccaggca gcaagccatc tggattttca gcgattcggg aatcggttcg ctgccagcaa
     4801 ggcaacgctc aatccactgc gccgtggttt ctgggtcttt tgcttgtggc agtaactcgc
     4861 taccagcagt gtcctgtttt tcatacagca cccgcatccc ttcacggtca atgagattga
     4921 tctgcgggca gcgctgcgga ttagcataca cttcgccttc agtaccatgc attagtagcg
     4981 cccgcccgcc gatatcgcta aagaacttcg cgactcgccc gatgtattcc ggatgcgaaa
     5041 cgctggaaag acgcagcgcc tcaccttcgg caaatggcgt cgccagtttc gccagggtat
     5101 gtgcactgtt acgcacgccc atccgccagc gcatcgccag ttgtttttcc agcggcgggc
     5161 aaaacgcgcc gacaggcata aacaccggtt gatgttcgtc gagcttcgcc tgcgcctgcc
     5221 cgccgtgcaa cgttggtgta atacccatca gttcgaaaat ggtttctgtc agcacgcgag
     5281 ttggatcttc gctaaccccg tgaaccacca caggaaaacc gagtttgtgc aggagaatag
     5341 ccaacaacgg cgtcaggttg gcctgtttac gcgcgccgtt gtaactgggg atgacaatcg
     5401 gcatcggctt gcctgcaggc ggcgtcagct tgatggtgtg attttgcatg gcttcgtaaa
     5461 agccgagcat ctctgcttcc ccttcccctt tgatacgtag cgcaatcaat acgccgccca
     5521 actcaaggtc agggacttca ccattgagca tatgggcata cagaccgcgc gcagtatccc
     5581 ggtctaaatc gcgcgcgtgg tttttcccgc gcccgatctc tttaatgatt ttgcgatagt
     5641 ccatttacga ctccttacct gactcacatc attaacgccg ccgacggcgt ggggatttcg
     5701 ttttttcttt ctttttaact ataccttcag ggacttccgg ttgctctatc ggaaataccg
     5761 gtaatgcatc cagtagtcgc ttgccataat ttttggtcag caagcgtttg tcgtagataa
     5821 ccacttcgcc ccagcaaccg tggcttcgaa tcagtcgccc aacctgctga atcaggttaa
     5881 acgaggcgct cggcaggctt tgcacctcaa acggatagcg gttgaggctt ttcagccatt
     5941 ccccttcggt gatcaccacc gggctgtcga tgggcggaaa agcgattttg tggatatgca
     6001 cctggctgag cagatcacct ttcaaatcaa gcccttcggc aaatgactgt aagcccacca
     6061 gcacactacg ctcaccgttg gcaacgcgtt tgcggtgcag ttcaactaag cgataacgcg
     6121 gctgatcgcc ctgcaccagc agcatcaggc gtaaatccgt cacatagtcg agaaagcgct
     6181 gcatcgcccg tccgctggca aacagtacca acataccgag atgttttttg ctctccacct
     6241 gctcacggaa aaaggccgcc atttcggcaa tatgctgctc ttcgttgtcg atggaaggct
     6301 caaagcgcat ccggggaata acaattttgc cctgttcgca gtggttaaag ggggaatcca
     6361 gcgccacaaa acggtcgccc gctttctctt tcagcccgct catctcctgc aaacgcgaaa
     6421 aactgttcag cgaacgcaag gttgcggagg tgacaataat gtgcggaata ctgcgccaca
     6481 gcagcctttc cagctgatcg ctgacacgta ttcccacgca gtgaaaccag agatgcagct
     6541 gcccttcgcg ctcttcccgc gtcgcccatt tggtcaccgg cgcaccggaa gattgcgcca
     6601 gagacgccag ccgccagagt ttgctttgcg cctcgaacat ccccaacgcg cggttcatct
     6661 gcaaaatcaa ccgatgcaga cgcacaatat cgtggctgcc ggttttctca cttaaatcgt
     6721 taagaaataa ctccgccaga ccacgcagca tctcggtgag ttttgccagc cgctggcaga
     6781 tctccagcac ttcatcaggc agttcgccca tcgcaaaacg gtgctctgcc tcctgcccgg
     6841 caggcatgta gagattgaga atgttgttta acgaggcgat aagctcatac aactcttcac
     6901 aatgcgcatt caaacgttca gggatcgcca gcggtgggat ggtcttcggg cgaaactgct
     6961 ccatgcaggt agcgaccagt ttcgtgaaca agtccagctg tagtcgatac cagggcgcgg
     7021 tgatttcagc gctcatctcc agcgcatccc gggccacatc tggcaggtga tgaccttcgt
     7081 ccagcaccag cagcagattt ttcgggtcag gcagtacggc ttcgctttcc atcgccgcca
     7141 tcaccagcgc atggtttgcc accaccactt ccgcttcctg aatctcccga cgagcaacaa
     7201 aaaacgggca ttcacgatag tagtaacagt tacggttaag gcagctggct ttgtcggtac
     7261 tcaaacgacg ccagagatca tcatctatag cgatatccgt atgatcgcgc agaccatccc
     7321 atttataagt gtcgagatcg cccttcagct tcgcacaacg tttttgctct tcctgattat
     7381 ttggcgtcag ttcgtcgtca agaaacgcca gcagatcctg ttgcgtgggt tcagtgctgg
     7441 cgagcgccgt cagattacgc ggacaaacgt agcgcccacg cccgaaagcg gcagtgaatt
     7501 taagatcggg aatgatcttt ttcagcagcg gtaaatcttt gctgtaaatc tgatcctgca
     7561 acgccacgtt ggcggtactc accaccagcg ttttttgctc ttcgcgggca atggcgatgc
     7621 cggggatcaa ataggagagc gttttcccaa cgccggtggg ggcttcaatc gccagatgcc
     7681 gcccttcttc tccggccagc gttttggcga cgtccgcaat catctgccgc tgcggcgcac
     7741 ggggaataaa gtcggggatc tgttcctgaa gcgccttata ccaggcggca atttgcgctt
     7801 taagcgcggc ggttaatgcc atgagaaaac ctgaaatact gtataaacag ccaatattgt
     7861 ggcatttttc ggttttcagg ggtaatggtt tttctgtttc tggaaagcgg ctcgcaataa
     7921 ccaccaacat aatgcggttt aatgcccgtt cgagcacaaa ggatccaaca cgtcatgcaa
     7981 gacaccatca taaatttcta cagcaccagc gatgactacg gtgatttttc taactttgcc
     8041 gcctggccca tcaaggttga cgggaaaacc tggcccacct ccgaacacta ttttcaggca
     8101 caaaaattcc ttgatgaaaa ataccgcgaa gagatccgtc gggtttcttc ccccatggtc
     8161 gccgctcgca tggggcgtaa tcgttccaag ccacttcgta aaaactggga gtcggttaaa
     8221 gaacaggtga tgcgtaaagc acttcgtgct aaattcgaac agcatgcaga actgcgtgcg
     8281 ctcttgctgg caaccgcgcc cgcaaaactg gttgagcata cggaaaacga tgcttactgg
     8341 ggagacggtg gtaatggtaa gggcaagaat cgactgggct accttttaat ggagttgcgc
     8401 gaacaattgg ctatagagaa gtaacgtctc ggtggcttat gggcggtaaa acagataaat
     8461 tttttcccgt gttctcaaat tgctgacaag tgcttgttgt gaaatgccgg atgcggcgta
     8521 aacgccttat ccggcctaca aaagcatgca aattcaatat attgcagagt taacgtaggc
     8581 ctgataagcg tagcgcatca ggcagttttg cgtttgttca tcagcctgat gccgggcata
     8641 gcccggcata aaagattact gcgcagcggc aggtttacgc ggacggcggc gacgttgttg
     8701 ttcgcctgcc ggtttggcat cgccgaggcg gcgagacggt ttttctgcag gtttcgcgct
     8761 tgcagatttt gcgccacctt ccccacggcg tggttgctgt tgaccgcgac caccaccttg
     8821 cccacgaccg ccgccgccac gttgctggcg accgttctgg atcggttcgg ctttgattga
     8881 cgggtccggc tcatagcccg gaatcgcaat gcgcgggatc tcttttttca gcagtttttc
     8941 gatatcacgc agcagtttgt gttcatcaac acacaccagc gacaacgctt caccggtagc
     9001 agccgcacga ccggtacgcc cgatacggtg gacataatct tccggtacgt ttggcagttc
     9061 atagttgacg acgtgcggca actcttcgat atccagaccg cgcgcagcga tatcggtcgc
     9121 caccagtacg cgaatgtcac tcgatttaaa atcagccagc gcacgggtac gcgcaccttg
     9181 cgatttattc ccgtggatcg ccgcactacg gatgccatct ttattgagct gctcagccag
     9241 atggttagcg ccgtgtttgg tacgggtaaa caccagcacc tgctgccagt tccctttacc
     9301 aatcatgtgc gacagcaatt cgcgtttgcg tttcttatcg acaaagtgaa cgtgctgggt
     9361 cacctgatca gacgcggtat tgcggcgtgc cacttcgatt tccagcgggt tgtgcaacag
     9421 tttttccgcc agggctttaa tatcgtcaga gaaggtcgca gagaacagca ggttctggcg
     9481 tttcgctggc agttttgtta acacgcgacg gatatcgtgg ataaagccca tgtcgagcat
     9541 gcggtcagct tcatcgagga cgaggatttc aacctgatcc agcttcactg cattttgatg
     9601 ttccaggtcc agcaaacgtc ccggtgttgc caccagaaca tcaacgccgc cgcgcagctt
     9661 catcatttgc gggttaatgc tgacaccgcc aaacaccacc agcgaacgaa tgttcaaata
     9721 tttgctgtaa tcacggacgt tttcgccaat ctgcgcagcc agttcacggg tcggggtaag
     9781 aatgagcgca cgtaccggac gacgcccttt ggcgtgcggc tggcgagtga tcaggtgttg
     9841 caacagcggc agcgtaaagc ccgctgtttt accggtaccg gtctgggcgc tagccatcag
     9901 gtcgcggcct tccagcaccg cagggatcgc ctgctgctga ataggggtgg gttcacggta
     9961 accctgctcg gcaacggcgc gcaggatatc agggcttaaa cccaaagaat cgaaagacat
    10021 aactactccg aaccgccccg accgttaccg gtgtagtttt cagggagata ctgaaaagga
    10081 aaatgacaaa aaccacaatg tcatgaggga gcggagtgta gcagtttttg tgacgcagcg
    10141 cataaattat ccctgtgact ggcgttgcta aaattcttag ccatactgga caactcccct
    10201 atggagtcat aatcttaatc aatcatttga ttaagaatgg actgtgcgat gaataatcct
    10261 gccatgacaa tcaagggtga acaggcgaaa aaacagctga ttgctgccgc actggcgcag
    10321 tttggtgaat atggaatgaa caccaccact cgcgagatag ccgcccaggc cgggcagaat
    10381 atcgctgcca tcacctacta cttcggttcg aaagaagatt tgtacctcgc ctgcgcccag
    10441 tggattgccg attttattgg cgagcagttc cgtccgcatg ccgaggaagc cgaacgcttg
    10501 ttcgcgcaac cacagcctga tcgggctgcc atccgtgaac tgatccttcg cgcctgcagg
    10561 aacatgatta agctgctcac ccaggatgac accgtcaacc tcagcaagtt tatctcccgt
    10621 gagcagctct ctcccacggc agcctaccac ctggtgcacg aacaggtgat tagtccgcta
    10681 cacagccacc tgacacgcct gattgccgcc tggaccggct gcgacgccaa tgacacccgc
    10741 atgatccttc atacccatgc gttgattggt gagattctgg cgttccgtct tggcaaagaa
    10801 acaattctgt tacgtaccgg ctggaccgcg ttcgatgaag aaaagaccga actgatcaac
    10861 cagacggtga cctgtcatat cgacctgatt ttgcaaggat tatcgcaaag gagtttgtag
    10921 tgatgaaaaa acctgtcgtg atcggactgg cggtagtggt acttgccgcc gtggttgccg
    10981 gaggctactg gtggtatcaa agccgccagg ataacggcct gaagctgtat ggcaacgtgg
    11041 atattcgtac ggtgaatctt agtttccgtg ttggggggcg cgttgaatcg ctggcggtgg
    11101 acgaaggtga tgcgatcaaa gcgggccagg tgctgggcga actggatcac aagccgtatg
    11161 agattgccct gatgcaggtg aaagcgggcg tctcggtggc acaggcgcag tatgacctga
    11221 tgcttgccgg gtatcgcgat gaagaaatcg ctcaggccgc cgcagcggtg aaacaggcgc
    11281 aagccgccta tgactatgcg cagaacttct ataaccgcca gcaaggactg tggaaaagcc
    11341 gcaccatttc ggcaaatgac ctggaaaatg cccgctcctc gcgcgaccag gcacaggcaa
    11401 cgctgaaatc agcacaggat aaattgcgtc agtaccgttc cggtaaccgt gaacaggaca
    11461 tcgctcaggc gaaagccagc ctcgaacagg cgcaggcgca actggcgcag gcggagctga
    11521 atttacagga ctcaacgttg atagcaccgt ctgatggcac actgttaacg cgcgcggtgg
    11581 agccaggcac ggttctcaat gaaggtggca cggtgtttac cgtttcacta acgcgtccgg
    11641 tgtgggtgcg cgcttatgtt gatgaacgga atcttgacca ggcccagccg gggcgcaaag
    11701 tgctgcttta taccgatggt cgcccggaca agccgtatca cgggcagatt ggtttcgttt
    11761 cgccgactgc tgaatttacc ccgaaaaccg tcgaaacacc ggatctgcgt accgacctcg
    11821 tctatcgcct gcgtattgtg gtgaccgacg ccgatgatgc gttacgccag ggaatgccgg
    11881 tgacggtgca attcggtaac gaggcaggac atgaatgatg ccgttatcac gctgaacggc
    11941 ctggaaaaac gctttccggg catgggcaag cccgccgtcg cgccgctcga ttgtaccatt
    12001 cacgccggtt atgtgacggg gctggtgggg ccggatggtg caggtaaaac cacgctaatg
    12061 cggatgctgg cgggattact gaaacccgac agcggcaatg ccacgatgat tggctttgat
    12121 ccaatcaaaa acgacggcgc gctgcacgcc gtgctcggct atatgccgca gaaatttggt
    12181 ctgtatgaag atctcacggt gatggagaac ctcaatctgt acgcggattt gcgcagcgtc
    12241 accggcgagg cacgtaagca aacttttgct cgcctgctgg agtttacgtc tcttgggccg
    12301 tttaccggac gcctggcggg caagctctcc ggtgggatga aacaaaaact cggtctggcc
    12361 tgtaccctgg tgggcgaacc gaaagtgttg ctgctcgatg aacccggcgt cggcgttgac
    12421 cctatctcac ggcgcgaact gtggcagatg gtgcatgagc tggcaagcga agggatgtta
    12481 atcctctgga gtacctcgta tctcgacgaa gccgagcagt gccgtgacgt gttactgatg
    12541 aatgaaggcg agttgctgta tcagggggaa ccaaaagccc tgacacaaac catggccgga
    12601 cgcagctttc tgatgaccag tccacacgag ggcaaccgca aactgttgca acgcgccttg
    12661 aaactgccac aggtcagcga cggcatgatt caggggaaat cggtacgtct gatcctcaaa
    12721 aaagaggcca caccagacga tattcgccat gccgacggga tgccggaaat caacatcaac
    12781 gaaactacgc cgcgttttga agatgcattt attgatttgc tgggtggtgc cggaacctca
    12841 gaatcgccgc tgggtgcaat attgcatacg gtagaaggca cacccggcga gacggtgatc
    12901 gaagcgaaag aactgaccaa gaaatttggt gattttgccg ccaccgatca cgtcaacttt
    12961 gccgttaaac gtggggagat ttttggtttg ctggggccaa acggcgcggg taaatcgacc
    13021 acctttaaga tgatgtgcgg tttgctggtg ccgacttccg gccaggcgct ggtgctgggg
    13081 atggatctga aagagagttc cggtaaagcg cgccagcatc tcggctatat ggcgcaaaaa
    13141 ttttcgctct acggcaacct gacggtcgaa cagaatttac gctttttctc tggtgtgtat
    13201 ggcttacgcg gtcgggcgca gaacgaaaaa atctcccgta tgagcgaggc atttggcctg
    13261 aaaagtatcg cctcccacgc gaccgatgaa ctgccattag gttttaaaca gcggctggcg
    13321 ctggcctgtt cgctgatgca tgaaccggac attctgtttc tcgacgaacc aacgtccggc
    13381 gttgatcccc tcacccgccg cgaattttgg ctgcatatca acagcatggt agagaaaggc
    13441 gtcacggtga tggtcaccac tcactttatg gatgaagcag aatattgcga ccgcatcggc
    13501 ctggtgtacc gtgggaaatt aattgccagc ggcacgccgg atgatttgaa agcgcagtcg
    13561 gccaacgacg agcaacccga tcccaccatg gagcaagcct ttattcagtt gatccacgac
    13621 tgggataagg agcatagcaa tgagtaaccc gatcctgtcc tggcgtcgcg tacgggcgct
    13681 gtgcgttaaa gagacgcggc agatcgttcg cgatccgagt agctggctga ttgcggtagt
    13741 gatcccgctg ctactgctgt ttatttttgg ttacggcatt aacctcgact ccagcaagct
    13801 gcgggtcggg attttactgg aacagcgtag cgaagcggcg ctggatttca cccacaccat
    13861 gaccggttcg ccctacatcg acgccaccat cagcgataac cgtcaggaac tgatcgccaa
    13921 aatgcaggcg gggaaaattc gcggtctggt ggttattccg gtggattttg cggaacagat
    13981 ggagcgcgcc aacgccaccg caccgattca ggtgatcacc gacggcagtg agccgaatac
    14041 cgctaacttt gtacaggggt atgtcgaagg gatctggcag atctggcaaa tgcagcgagc
    14101 ggaggacaac gggcagactt ttgaaccgct tattgatgta caaacccgct actggtttaa
    14161 cccggcggcg attagccagc acttcattat ccccggtgcg gtgaccatta tcatgacggt
    14221 catcggcgcg attctcacct cgctggtggt ggcgcgagaa tgggaacgcg gcaccatgga
    14281 ggctctgctc tctacggaga ttacccgcac ggaactgctg ctgtgtaagc tgatccctta
    14341 ttactttctc gggatgctgg cgatgttgct gtgtatgctg gtgtcagtgt ttattctcgg
    14401 cgtgccgtat cgcgggtcgc tgctgattct gttttttatc tccagcctgt ttttactcag
    14461 taccctgggg atggggctgc tgatttccac gattacccgc aaccagttca atgccgctca
    14521 ggtcgccctg aacgccgctt ttctgccgtc gattatgctt tccggcttta tttttcagat
    14581 cgacagtatg cccgcggtga tccgcgcggt gacgtacatt attcccgctc gttatttcgt
    14641 cagcaccctg caaagcctgt tcctcgccgg gaatattcca gtggtgctgg tggtaaacgt
    14701 gctgtttttg atcgcttcgg cggtgatgtt tatcggcctg acgtggctga aaaccaaacg
    14761 tcggctggat tagggagaag agcatgtttc atcgcttatg gacgttaatc cgcaaagagt
    14821 tgcagtcgct gctgcgcgaa ccgcaaaccc gcgcgattct gattttacct gtgcttattc
    14881 aggtgattct gttcccgttc gccgccacgc tggaagtgac taacgccacc atcgccatct
    14941 acgatgaaga taacggcgag cattcggtgg agctgaccca acgttttgcc cgcgccagcg
    15001 cctttactca tgtgctgctg ctgaaaagcc cacaggagat ccgcccaacc atcgacacac
    15061 aaaaggcgtt actcctggtg cgtttcccgg ctgacttctc gcgcaaactg gataccttcc
    15121 agaccgcgcc gttgcagttg atcctcgacg ggcgtaactc caacagtgcg caaattgcca
    15181 ccaactacct gcaacagatc gtcaaaaatt atcagcagga actgctggaa ggaaaaccga
    15241 aacctaacaa cagcgagctg gtggtacgca actggtataa cccgaatctc gactacaaat
    15301 ggtttgtggt gccgtcactg atcgccatga tcaccactat cggcgtaatg atcgtcactt
    15361 cactttccgt cgcccgcgaa cgtgaacaag gtacgctcga tcagctactg gtttcgccgc
    15421 tcaccacctg gcagatcttc atcggcaaag ccgtaccggc gttaattgtc gccaccttcc
    15481 aggccaccat tgtgctggcg attggtatct gggcgtatca aatccccttc gccggatcgc
    15541 tggcgctgtt ctactttacg atggtgattt acggtttatc gctggtggga ttcggtctgt
    15601 tgatttcatc actctgctcg acacaacagc aggcgtttat cggcgtgttt gtctttatga
    15661 tgcccgccat tctcctttcc ggttacgttt cgccggtgga aaacatgcca gtatggctgc
    15721 aaaacctgac gtggattaac cctattcgcc actttacgga cattaccaag cagatttatt
    15781 tgaaggatgc gagtctggat attgtgtgga atagtttgtg gccgctactg gtgataacgg
    15841 ccacgacagg gtcagcggcg tacgcgatgt ttagacgtaa ggtgatgtaa cttcttatct
    15901 ttcgccagca aagacactac cgccgggccg gcaaggattg ccagccctgc aattgccagc
    15961 aaaaagttgt tttccagtac gcgggacaac agccacagca caatcagcgc tgcgccaaaa
    16021 taccaggtcg tggtcagctc ttccaacacg tctgccacct cacgccagcg ttgctcgggg
    16081 tggcgctgaa acaccaacat catcactacc gtgacacaat acagtgcgca taatcccacg
    16141 ccgacgtgaa ccagagtctt actcatccag cccaccacca gcagcgccac taccagtaaa
    16201 tgcaacataa tctgccagca acttagaccc gttgcgacac gaacacgttg ttgccacttc
    16261 atggcttctc tcctgaagaa tttatctcta cttactcaga gtaccgaact ttacggaaaa
    16321 ttccgtaaga ccgcaccttt ttttgacgcc gactacacta tttcaatgag ataagaggca
    16381 aagacaggaa aattatgccc gatcaaacac aacaattttc gttcaaggtg ctcaccatca
    16441 acattcaaaa aggttttacc gcgtttaacc gacgcttcat tttgccggaa cttcgcgacg
    16501 cggtgcgtac cgtcagcgcc gatattgttt gcctgcagga agtgatgggc gcgcacgaag
    16561 ttcatccgct gcatgtggaa aactggcccg atacctcgca ctacgagttt ctcgccgaca
    16621 ctatgtggag cgattttgcc tacggtcgca atgccgtata cccggaaggg catcacggca
    16681 acgccgtact gtcgcgttat cccattgaac attatgagaa tcgcgatgtt tcggtcgatg
    16741 gtgcggaaaa gcgcggcgtg ctctactgcc gcattgtgcc gccgatgacc ggaaaagcga
    16801 ttcatgtgat gtgcgtacat ctgggcctgc gtgaggcgca ccgtcaggcg cagcttgcga
    16861 tggggtgaat gagctaccgg acggcgaacc ggtattggtg gcgggtgatt tcaatgactg
    16921 gcggcaaaaa gctaatcatc cgttaaaagt gcaggccgga ctggatgaga tttttacccg
    16981 cgcccacgga cgcccggcgc gcacgtttcc ggtgcaattt cctctactac gactggacag
    17041 gatctacgtc aaaaatgcca gcgccagcgc gccaaccgcg ttgccgctgc ggacatggcg
    17101 acacctttct gatcatgccc ctttaagtgc ggagattcat ttatgaaatg tagctggcgc
    17161 gaaggcaata agatccagtt gctggaaaac ggcgagcaat attatcccgc ggtgtttaag
    17221 gcgattggcg aggcacaaga acgcatcatt cttgaaacgt ttatctggtt tgaggataac
    17281 gtcggcaaac agctgcatgc ggcactactg gcagcagcgc aacgcggggt taaagcggaa
    17341 gtcttgctgg atggctacgg ttcgccggat ctcagcgatg agtttgtcaa taaactgacg
    17401 gcagctggcg tagtgttccg ctactacgat ccccgccctc gcctttttgg tatgcgcacc
    17461 aatgtgtttc gccggatgca tcgcaaaatt gtggtgatcg acgcgcgtat cgcctttatt
    17521 ggcgggctga attactccgc cgagcatatg tccagctacg gtccagaggc taaacaggat
    17581 tacgcggtac gccttgaagg gccgattgtc gaagatattc tccagtttga gctggaaaac
    17641 ctgcctggac agagcgcggc acggcgctgg tggcgacgtc atcacaaagc ggaagagaac
    17701 cgccagccgg gagaagcgca ggtattgctg gtctggcgcg ataacgaaga acatcgcgat
    17761 gatattgaac gccattatct gaaaatgctc actcaggcgc ggcgggaagt gattatcgcc
    17821 aacgcctact tcttccccgg ctatcgattt ttacacgcct tgcgtaaagc ggcacggcgc
    17881 ggggtgcgga tcaaactgat cattcagggc gaaccggata tgccgattgt cagagtcggt
    17941 gcgcgcttgc tgtataacta tctggttaaa ggcggcgttc aggtttttga gtaccgccgc
    18001 cgcccgctcc acggcaaagt ggcattgatg gacgatcact gggcgacagt agggtccagt
    18061 aatctcgatc cgctcagttt gtcactgaat ctcgaagcaa atgtcatcat ccacgatcgt
    18121 cattttaacc agacgctgcg cgataatctg aacggcatta ttgccgcaga ttgtcagcag
    18181 gtggatgaaa ccatgctgcc caaacgcacc tggtggaacc tgaccaaaag cgtgctggcg
    18241 ttccactttt tacgccactt cccggcgctg gttggctggc atccggcaca cacaccacgt
    18301 ctgacgcagg ttgatccccc cgcacaaccg acaatggaaa cgcaggatcg ggtagaaact
    18361 gaaaacacgg gggtaaaacc ctgatgagta aatcacaccc gcgctagcgc ttagcaaaga
    18421 agatcctcac ctggctgttt tttatcgcgg tgattgtgtt gctggtggtc tacgccaaaa
    18481 aagtggactg ggaagaggtc tggaaggtca tccgcgacta caatcgcgtt gcgctgctta
    18541 gtgcggtcgg gctggtggtc gtcagctatc tgatttacgg ctgctatgac ctgctcgccc
    18601 gcttttactg cggtcacaaa ctggcgaagc gccaggtgat gctggtgtcg tttatctgct
    18661 acgccttcaa cctgacgctc agtacctggg tcggcggcat tggtatgcgc tatcgtttgt
    18721 actcccgact agggttaccg ggcagcacta ttacgcggat tttctcgctc agtattacca
    18781 ccaactggct gggctatatt ttactggcag ggattatctt taccgcaggc gtggtggtgt
    18841 tgccggatca ctggtatgtc gctcaaacta cgctgcgtat tctcggcatt ggcttactga
    18901 tgattatcgc ggtttatttg tggttttgcg ctttcgcgaa gcaccgccat atgaccatca
    18961 aagggcaaaa gctggtgctg ccttcatgga aattcgccct cactcagatg ctgatttcca
    19021 gcgttaactg gatggtaatg ggggcgatta tctggctgtt acttggtcaa agcgtgaact
    19081 atttctttgt actgggcgtg ttactggtta gtagtattgc tggcgtcatc gtgcatattc
    19141 cggcggggat cggtgtgctg gaagcggtgt ttatcgcact actggctggg gagcatacct
    19201 cgaaagggag cattatcgcc gcgctgctcg cttatcgcgt cctgtattac tttatcccgc
    19261 tgctgctggc gctgatctgt tacctgctac tggaaagcca ggcgaagaag ctacgggcga
    19321 aaaatgaagc ggcgatgtga aatttagtta agttctttag tatctgcatt cacgactaat
    19381 gaaaaaaacg cgtatgcctt tgccagacaa gcgttatagc tatcgcggca agtatcccaa
    19441 agaagccgat aaaatagtaa tagagtgtca atgcacctgt aaaagcgcaa cgaaccggac
    19501 tccacaactt ttcactatgg cattttcctg ccagttcaag gagcttattt cgtccatgcg
    19561 agataatgcc tggccataac acaatcacta agcaacttat tacccaaatg ggccgttcac
    19621 tcattaatgc cgcattcacg atgattacca gggccaaccc tgtcaacgtc atcataatga
    19681 taaaacggtg agaaccagga tcaacattaa ataataaacc ggccagcata ctgattaaaa
    19741 acatcgcgcc cgcagtgctg aacacaatga taatggcata tacaggaact aaacttgcaa
    19801 cgacaatcgc caccaaagag agtaaaaaca gatatattac gcctgcgatg acggcattta
    19861 tcttgttgat atccttactt aagccataca tacaaactaa taataaccct atggcgacat
    19921 acattgttgg tgaatcatat ttggttgccg taatttcgag atacttagct ccccaggcca
    19981 gtcctgcggt tgccaccaaa cctaaagcgg taatgagcca tataccgttt aatatcctgg
    20041 tctcgtgctg aaaatctaat ttattgctgt tttgtgagta tgattccatt cttttagttg
    20101 tccttagaaa aaatattaat attcaccatc aataaattta aacccacgca tacaaaatac
    20161 ttttgtattt atggatacgg aaaacgcttc cgctcggcca taaggcgtat tttatacctg
    20221 gtacttgtta agtgtcgttt ttagtaagaa aagaaatccc gacacatggc cgggatattg
    20281 atggtgaaaa agaattaacg gcggttgcca aaaattcgca gcaacatcag gaacaggttg
    20341 atgaagtcca gatacaaggt taacgcgcca agaatggaat atttgcgcag gttcgacgtg
    20401 tcgcgggtat caatctgctc acccatattt ttcaatttct gagtgtcata cgccgtcaat
    20461 ccgacaaaga caatcacgcc gatgtaggta actgcccaca tcaatgcttc gcttttcagc
    20521 cagaagttga ccagcgatgc cagcacaatg ccgattaacg ccataaacag catattgcca
    20581 aagccactta aatcgcgctt cgtggtgtaa ccgtacaggc tcattgcgcc gaacatcccg
    20641 gcagtaacga cgaaagtact ggcgatagaa gcagcggtat agacaatgaa tatactggaa
    20701 agcgtaagac ccgtcagcgc cgaataaagc ataaagagca tcgtcgttac acctgcgctc
    20761 agcttttgaa tcatcgctga taacacaata accaatgcta attgcgcgat gatcagaccg
    20821 attaaaaaga cacggttagt gaacaacagc tccatcacgg ccgcggaatt agccgcatac
    20881 caggcaacaa atgcggtcag caacaagcca acggtcatcc agccatagac ttgcgccata
    20941 taagtttgca agccagcccg gggttgtacg attgaatcag aacgtgggaa tctgtccatg
    21001 acgatctcct gaagatataa ggaatatctt aaggatactg caaaatgatg aggctgtgca
    21061 tcgacgcagc gtaaacgcat gtactgagcg gtgaaattgc cggacgcagc ggtgccttat
    21121 ccggctaaca aaaaactacc agcgttttgc cgcctgctga tcgctctccc gagcttcaac
    21181 ccagcggtcg ccttccggcg tggcttcgcg cttccagaac ggtgcgcggg ttttgagata
    21241 atccataata aactgcccgg cttcaaacgc actgctgcga tgcgcactgg tgacaccggc
    21301 aaaaacgatt tcatcgcccg gccataattc cccgatgcgg tgaatcacag tgacgcgccc
    21361 cagcggccag cggttacgcg cttcatcaac aatttctgcc agtgcttttt cagtcattcc
    21421 cggatagtgt tcgagggtta atgctttgac gctgtcgccc aggttatggt tgcgcacctt
    21481 accagtaaag gtgactaccg caccgtcttc gtcacgctcc gccagccacg ggtactcttc
    21541 tcctacgctg aacggctgcg gaccaacaac aattttggtt tctgccatct taacctccgg
    21601 ttaccggcgg gaagaaagct acttcgtcgc cgtcagtcag cggatggtca aaactcacca
    21661 gcgtctggtt gacggcagcc agtaatttgc catcttccag cgccagcgcc cagcgatcgc
    21721 tctgcgcagc catgtgctgg cgtaacgctt caacagttgg gaaatccgca gccacttcgg
    21781 ttgcatctgt tcccaccaac tcgcgcacct gcgcgaaaaa aagaacttta atcatccgct
    21841 tccaccttaa agtcacccga cttgccgccg ctcttcgcca gcaaacgtac cggaccaatc
    21901 accatatctt tttgcaccgc tttgcacatg tcataaatgg tcagcgccgc cacggaggcc
    21961 gcggttaatg cttccatttc gacaccggtt ttcccggtca ggcggcataa ggtttctata
    22021 cgcacccgat tgtgctccgg ctcggcctgt aaattgactt caactttgct gagcatcagc
    22081 ggatgacaga gcgggatcag atcccaggtg cgttttgccg cctgaatacc ggcaatacgc
    22141 gcagtggcaa atacgtcgcc tttgtggtgg cgaccatcaa taatcatcgc cagcgtctcg
    22201 ctgcgcatgg tgacaaaggc ttccgcccgc gcttcacgca cggtttccgc tttggcggag
    22261 acatccacca tgtgcgcttc gccagcggcg ttgatatggg tcagttgcga catacttatt
    22321 tcttcaaatg tggatggaaa ttacacggac gcgtacgggc atccagctgc ggcgcgatga
    22381 tattttccca tgcggtacgg cacgctttgg tcgaacccgg catggcgaaa atcagcgttt
    22441 tgttggcgac gcccgcaacc gcacgagatt gcaacgtgga agtgccaatc tcttcaaacg
    22501 acaacatccg gaacacttca ccaaaacctt caacttcacg gtcgaacaac ggcagcaatg
    22561 cttcgggagc ctgatcacct tccgtcaggc cagtaccacc cgtaatcaat accacttgta
    22621 catcgtcgct ggcgatccac gcagatacct gagcgcgaat agcgtagcgg ttttctttca
    22681 caatggcttt atcgacaacg tgatggcccg cttcttgcgc cgaatcgcgc agatagtgac
    22741 cggaggtatc gtcttcttca ccgcgacgat tagaaaccgt aagaatagca atacgggtcg
    22801 ggataaattc agtgcttacc tgactcatct gatctctcct tttgacgttt tagccgccaa
    22861 tgtacgataa gttttgcgta ataccggtgt tgttttgatg caggaaatgg gtctgtttct
    22921 tctcccgcag cgccgctgaa atacgcgctt ccagcgcctg ttgctgggta tcgtcttcca
    22981 gcagatcgcg caggttaacg ccgccttcac caaacaggca gagatggagt ttaccaatgg
    23041 aggaaacgcg caggcggttg caagtggcgc agaagtcttt ttcatacggc atgataaggc
    23101 caatctctcc ggcgtaatct ggatggcaaa agacttgcgc gggaccgtcg ctgcgttgac
    23161 gtaattggtg gatccagccg cgacgcagta gctcgtcacg cagaacctga ccagagatgt
    23221 gatgcttacg gaagagctcg ctgccctcgc ccgtttccat cagttcgatg aaacgcagct
    23281 ggatagggcg atgctggatc cagttcagaa aggtgtcgag ctggtgatga ttaacatcac
    23341 gcatcagcac ggtattgact ttgaccttct caaaaccggc ctcaaatgca gcatcaatcc
    23401 ctgccatgac ctggttgaat ttatcctgcc cggtaatagc gtgaaactgg cgggcgtcca
    23461 gactgtcgac actgacgtta atgccagtaa gtcccgcatc gcgccagttc gccacatcgc
    23521 gttccagacg gtaaccattg gtggtgaccg caatctggcg gatagcgtcg ttttcccgca
    23581 cagcggcgat gatatcggta aagtcgcggc gtaaagacgg ctcacctcct gtcaggcgca
    23641 ctttttcggt gcccagactg gcgaaggcgc gcgtaacccg gcgaatttca tcgacggtaa
    23701 gaaagccttt attggtgacg ccgctcggtt tgtagccatc cggcaggcag taggtgcaac
    23761 gaaagttaca cacatcggta atcgacaggc gcaagtagta aaacttacgc gcaaatgcat
    23821 cagtcagttg tgaagccatg tacacctttc cagatacggg aggcgaagtc atttcttcct
    23881 tcgccctggt ggcaatcttt acgagcattg tcacggccaa agcaccgtat cagttgaccc
    23941 aggtgcagag gctagagtgt ttagtggtta agccgatact agcgtgtaaa tgagattttt
    24001 accatccaca ctttcgctat gcaatcatgt atatagcgtc atgatcgtgc tatttcgcta
    24061 cagaataagg gaaaatggca gttttacttt gatatacatc atcagggagg catagcgtgc
    24121 atcgtccttt agaggtagaa gaaaatagtt tgtatcctca atattggcag gttaattgct
    24181 gtttccccgc aatttgcgct actgtagcgc ggataatctg actccaggaa actatatgcg
    24241 caatcgtacg ctggctgatc ttgatcgtgt cgttgctctc ggcggagggc atggactggg
    24301 acgcgttctc tcatcacttt cgtctttggg ttctcgttta acgggtatcg tcaccaccac
    24361 ggataatggt ggctcgacgg ggcgtattcg ccgttcagaa ggcggcattg cctggggcga
    24421 tatgcgcaac tgcctcaacc aactgataac ggaaccgagc gtcgcctccg cgatgtttga
    24481 ataccgtttt ggcggcaatg gcgaactttc cgggcacaac cttggaaact tgatgttaaa
    24541 ggcgctggat caccttagcg tgcggcctct ggaagccatc aatttaattc gtaatctgct
    24601 gaaagtggat acgcatttga ttccaatgtc agagcatccc gtcgatctga tggcgattga
    24661 cgatcagggg catgaagttt acggcgaggt caatatcgac cagttaacta cgccgattca
    24721 agagttatta ttaacgccga atgtacccgc aacgcgtgag gcagttcacg ctatcaatga
    24781 agcggatctc atcattattg ggcctggcag tttttatacc agcctgatgc caattctgct
    24841 gctgaaggaa atcgcccagg cattacgccg cacgccagcg ccgatggttt atatcggcaa
    24901 tctggggcgt gagttgagtt tacctgcggc taatttgaag ctggaaagca agctggcaat
    24961 tatggagcag tatgttggta aaaaagtcat tgatgcggtc atcgtcgggc caaaagtgga
    25021 tgtctcggcg gtgaaagagc ggattgtgat ccaggaggta ctggaggcca gcgatattcc
    25081 gtatcgtcat gaccgccagt tgttacataa cgcgctggaa aaggcgttac aggctttagg
    25141 ttaacggaaa tccgaaggaa aattccggct tcctattgaa gacaaagtgc gcgttgttta
    25201 tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc gcgcaaattc aatatattgc
    25261 ggagaaaatg taggcctgat aagcgtagcg catcaggctg ttttccgttt gtcatcagtc
    25321 ttcttcgcta tcctgttacg atgcggcaat aaacaactca cgcaactgat gcaactggtc
    25381 acgaatttgt gccgcttctt cgaactccag attctgcgcg tgttgcatca tcagcccttc
    25441 cagttcatgg attttctgct gcaacgcttt cggcgacata tccatcggca cattatccgg
    25501 ctcaacaatc gggcgcgatt ttcctctgcc cttcgctttg gttttggcaa tgttctgccc
    25561 cagcgccagg atatcgacca ctttcttgtt caagccttgc ggcgtaatgc cgtgttcctc
    25621 gttgtacttc tgctgtttct cacggcgacg ttcggtttcg ccaatcgctt tcgccattga
    25681 tggggtgatc ttatcgccgt agagaatcgc tttaccgtta acgttacgtg ccgcacgacc
    25741 aatggtctgg atcaacgaac gttcggaacg caggaagcct tctttgtcag cgtcgaggat
    25801 cgccaccagc gacacttccg gcatatccag accttcgcgc agtaagttga tccctaccag
    25861 cacgtcgaac tcacccagac gcaagtcgcg gataatctcc atacgttcga cggtgtcgat
    25921 atctgagtga agataacgca cgcgctcgcc atgttcttcg agatattcgg taagatcttc
    25981 cgccatccgc ttggtcagtg tggtgaccag tacgcgttcg ttaattgccg ctcgctgacg
    26041 aatctccgaa agaagatcat caacctgtgt cgccaccggc cgcacttcga taatcgggtc
    26101 aagcaatccg gttggacgca ccacctgatc caccacatcg ccgccggatt tttccagctc
    26161 gtaattaccc ggcgtcgccg aaacatagat ggtttgcggc gctaatgctt cgaactcttc
    26221 aaacttaagc ggacggttat ccagcgctga tggcaggcgg aagccgtact ccaccagtgt
    26281 ctctttacgc gcccggtcac cgcgatacat gccgccaatt tgtggaatgg tgacgtgaga
    26341 ttcatcgacg accagcagcc catcggcagg caggtaatca aacagcgtcg gcggtggctc
    26401 acccggtcca cgaccggaga ggaagcgcga gtagttttca atccccgaac agtagcccag
    26461 ctcgttcatc atctccagat caaactgggt acgctgggtc agccgctgct cttccagcag
    26521 tttgttgttt tccaacagca ctttgcgtct ggcggccagt tcttctttga tctcctccat
    26581 cgcctgtacg atgcgctcgc gcggtgtgac gtagtgcgtt ttcgggtaga tggtaaaacg
    26641 tggaatagtg gaaacaatct gcccggtcag cgggtcaaat aacgacaatc gttccacttc
    26701 ctcgtcaaac agttccacgc gaagtgcaat gtcatccgat tctgccggga agatatcaat
    26761 cacctcgcca cgaacgcgga aagtaccacg ctggaatgct tgatcattac gagcgtattg
    26821 cagctccgcc agtcggcgca gaatcgcgcg ctgatcgata atcataccga ccgtgagatg
    26881 gagcatcatc ttgagatata aatcaggatc gcccagacca taaatcgcgg aaacagaggc
    26941 cactacaacc acatcacgcc gctccagcat cgctttggtg gcggataaac gcatctgctc
    27001 aatatgttcg ttaaccgagg catctttctc aatgaaagtg tcggaactcg gtacataggc
    27061 ttccggctga tagtagtcgt agtaggagac gaaatactcc accgcgtttt ccgggaagaa
    27121 ctctttcatt tcgccataca gctgggccgc cagcgttttg ttgggcgcaa gtaccatggt
    27181 tgggcgctga aggtcagcaa tgacattggc aatggtgaag gttttccctg agccagtcac
    27241 gccaagtaac gtctggtgcg ccaggccatc ttccagcccc tcttcgagac gtcgaatcgc
    27301 ctctggctga tcgccagaag gtttaaaagc ggaattcagt ttgaacggtt tactcatgag
    27361 tcgctacctg aaggagttgg gcgggcaggt atgtaatttt actcgtcgta cttaattatg
    27421 ccaacaaatt atactggata aaaaaacagt tcatcaccat aatatttctg atacagcgta
    27481 aactccgctg tcaatcatga gcaaaattta ctctgtggcg agataaaact ccggccttac
    27541 cgggttatcc ccaaagcaac ggctttttta acatttgtca agatgagtga tgacagtttt
    27601 atggcaagag atgcctgttc agtgactgcc attgcattta tctaaccagt taaaaaataa
    27661 aagatatttc tttgagccgt ctttaacgcc aattcgcgtg caagccgcgt attctctcgc
    27721 ttgcctcgtg ttttctaact ctaatacaca tggttatcca caggaatagt ggataactgt
    27781 ctccagcccc tatcccccgc cgcctgggca attcccacat tcgccgcatg aggcggaagt
    27841 aaaatttttt tcgtgattat tttagaattt aattggttaa attgcagtca atcgaagacg
    27901 cgatctcgct cgcaatttaa ccaaatacag aatggctaca acaaggcaag gtttatgtac
    27961 tttccggttg ccgcattttc tggattttct gcaagccagg ggatctctcc cagcagcggc
    28021 gcgggaatca tgcgggtgag cgtggtcata tattcagcgt gacgttttcc cggaggcgta
    28081 acatcgttcg ccacccaacc cgccagagtc agtccggcgt gttgtattgc ctgcgcagtc
    28141 aacatcgcgt gattaataca gccgagtttc acaccaacta ccagtatcac cggcagttgt
    28201 tcctgtgtta cccaatctgc aaaagtgaaa gtgtcagaaa gcggcgtaaa ccagccgcca
    28261 gcaccttcca ctaacaccca gtcagcctgt tgttcaagcg cgcgtaatcc ggcgctcatt
    28321 accaatgatt ctatcggcct gccctcttgc gcgctgatga tgtgcggcga agtgggttct
    28381 gcgaaggtgt aaggatttac tgttgcgtaa tccagctgca ggctgctgtt gtgctgtaac
    28441 gccagcgcgt cactattacg taagccttcc ggggtctttt cgctgccaga ggcgaccggt
    28501 ttataacctg ccgtccgata acctactgcc ttcgcggctt gtaaaagtgc acaactggcg
    28561 acggttttcc ccacttcggt atccgttccg gtgacaaaat aacgtttact cacgagcaat
    28621 cactcccaaa aaaagatgat acgtcagagg atatcgcccc tgttgttgcg gccaggccag
    28681 ttgcaatcgc tgcaattgcg aacgcgttaa tattcgcggg tcgcgccctt catgaagatg
    28741 cgtagcaccg atgcctttca gcgaacgcat ggcactgagc gcatcatcaa accatagtgt
    28801 gatgggctga atatgatgtt gataatgcac gccgttcagc gactgttcga tttcatctgg
    28861 cggtaaaaag cgattagcat gcggacgctc gtccaccgcc tgccacgcct gatgcagttc
    28921 gggtaacgat ccctgcacca gcgtggtaaa cgccaccacg cctccggggc gcaccacccg
    28981 atacagctcg cggagtgccg tggataaatt accgcaccac tgcaccgcga gattgctcca
    29041 tgcaagatcg aacgtcgcag tcgctaacgg cagggattcg atatctcccg ccagataatg
    29101 gtctgcagca tctttctggc gggcttgcac aagcattggc ggtgagagat ctaacgccgt
    29161 cacctgtgcg tgacgctctc gccagtggcg gctcatccag cctggcccac accccgcgtc
    29221 cagtacgtgg gtgtatttac gctgtgggag cattgccagt aagacgtcag cactccggcg
    29281 ctgtaactct gcgtgttgct catagtgcgc ggctgcccga ccaaatgccg ctgcaatggc
    29341 ttgtttatta accgttgcca tgcagcacct ccagcagacg gtcgatatcc tgcatttcat
    29401 gcgctgcggt tagcgttaag cgcagtcgcg cagtaccagc gggtacggtt ggcgggcgaa
    29461 tcgccgtgac ccagcagcct tgctggcgca gtttttctgc cagttgtaac gcacggctgt
    29521 tatcaccgac gatcaatggc tggatggcgc tgcatgaatc agcaagcgta aacggcaaat
    29581 cctgtactcc ggcacgaaaa cgcgcaatga gtgacaccag tttttcgcgc cgtgcatccc
    29641 cctcatcacg gcgaatgacc gccagcgacg cacgtaatgc ctgcgcctga gcgggcggca
    29701 tactggtgtt gtagataagg tggcgggcga attgcagcag ataatccgcc accgtactgg
    29761 agcaaagcac cgctgccccg ctgacgccaa atcctttgcc aaaagtcacc accagcaatt
    29821 ctggttttac cttttgcagc cagcagctgc cgcgcccctg ctccccgata acgcccgtgc
    29881 cgtgggcatc atcgaccatc aaccagccat tgtgctgttg cgttacctgc tggatttccg
    29941 ccagtggcgc actatcgccg tccatgctga acacgccttc tgtcaccacc agttgctgcc
    30001 ccggacaggg ggaagcaagc agtcgcgcca gatgagtgac atcgttatga acaaaacggc
    30061 gaagctgcga cgggcttaaa ctggcagcct ccagcaatga ggcatggcta agccggtcag
    30121 cgacaatgcg gtcctctttt gccatcatcg cggcaatgac cgcctgatta gcggcaaaac
    30181 cagagataaa tagcagtgcc cgcgaatagc ccagccactc ggccagttct tcttccagcg
    30241 cctgatgcgc aacgctataa ccgctgacgt gaccggaacc gccgctaccg acgccaaatt
    30301 gctccgcacc ctgctgccag gcacggataa tttgcggatg atggcttaaa cctaaataat
    30361 cgttactgga aaagttcaga tactggcgat catccgccac cagccagcgt ccggctcctt
    30421 gcgccactgg ataacggcga cgcagggcat cggcagcacg ccgcgcatcg agcgccgcgt
    30481 tgattttctc ctgccagctc atagtgctgc cgcgttgtaa tattcgtcag tatccggggt
    30541 catcagcgcc tgttcaagac gttgctgttg ttcgttatcc cctgccagca cggcagtttg
    30601 ctgcggattt agccccagtt tgcggaacag ttgcaggtct ttatcttctt ccggattcgg
    30661 cgtggtcagc agtttgcaac cgtagaaaat cgagtttgcg cctgccataa agcacatcgc
    30721 ctgagtctgt tcgttcatct gctcgcgtcc ggcagaaagg cgcacgtaag aggtcggcat
    30781 catgatccgc gcgaccgcaa tggtgcgaat aaaatcaaag gcatcaacgt catcgttatc
    30841 ggcaagcggc gtgcctttca ccttcaccag catgttgatt ggcacgcttt ccggcggcgt
    30901 cggcaggttt gccagttgca gcaataatcc ggcgcgatct tttaccgttt cgcctaagcc
    30961 cacaatgccg ccagaacaga ctttgatccc ggcatcgcgc actttttcca gcgtatcgag
    31021 gcgttcctga taagtgcggg tggtgatgat attgccgtaa aactccggcg aggtgtcgag
    31081 gttgtggttg tagtaatcca gcccggcgtt cgcgaggcgc tgcgcctgag attcactcaa
    31141 cgtgcccagc gtcatacacg cctccagccc taaatctttt accccctgca ccatctgctc
    31201 caggtacggc atatcgcgtt cgttgggatt cttccacgcc gcgcccatgc aaaaacgcgt
    31261 cgatcccgcc gctttcgctt tgcgcgccga ctccagcacc tgttcaactt ccatcaaccg
    31321 ctcggcttcc agaccggttt tatagcgcga gctttgcggg caatatttgc aatcttccgg
    31381 gcaagcaccg gttttaatcg acagcaacgt gctgacctgc acctgacgag gatcgaaatg
    31441 ctggcgatgc acctgctgcg cttcaaacag cagatccagc aacggttttt caaataattc
    31501 tgcgacttgc gacaatgtcc agcgtgggcg gtgagccatg gggcttctcc aaaacgtgtt
    31561 ttttgttgtt aattcggtgt agacttgtaa acctaaatct tttcaatttg gtttacaagt
    31621 cgattatgac aaaggacgat cttgcctttg accaacgcca tatctggcac ccatacacat
    31681 ccatgacctc cccgctgccg gtttatccgg tggtgagcgc cgaaggttgc gagctgattt
    31741 tgtctgacgg cagacgcctg attgacggta tgtcgtcctg gtgggcggcg atccacggct
    31801 acaatcaccc gcagcttaat gcggcgatga agtcgcaaat tgatgccatg tcgcatgtga
    31861 tgtttggcgg tatcacccat gcgccagcca ttgagctgtg ccgcaaactg gtggcgatga
    31921 cgccgcaacc actggaatgc gtttttctcg cggactccgg ttccgtagcg gtggaagtgg
    31981 cgatgaaaat ggcgttgcag tactggcaag ccaaaggcga agtgcgccag cgttttctga
    32041 ccttccgcaa tggttatcat ggcgatacct ttggcgcgat gtcggtgtgc gatccggaca
    32101 actcaatgca caacctgtgg aaaggctatc tgccagaaaa cctgtttgct cccgccccgc
    32161 aaagccgcat ggatggtgaa tgggatgaac gcgatatggt tggctttgcc cgcctgatgg
    32221 cggcgcatcg tcatgaaatc gcggcggtga tcattgaacc gattgtccag ggcgcaggcg
    32281 ggatgcgcat gtaccatccg gaatggttaa aacgaatccg caaaatgtgc gatcgcgaag
    32341 gtatcttgct gattgccgac gagatcgcca ccggatttgg tcgtaccggc aaactgtttg
    32401 cctgtgaata tgcagaaatc gcgccggaca ttttgtgcct cggtaaagcc ttaaccggcg
    32461 gcacaatgac cctttccgcc acactcacca cgcgcgaggt tgcagaaacc atcagtaacg
    32521 gcgaagccgg ctgctttatg cacgggccaa cttttatggg caatccgctg gcctgcgcgg
    32581 ctgcaaacgc cagcctggcg attctcgaat ctggcgactg gcagcaacag gtggcggcta
    32641 ttgaagtaca gctgcgcgag caacttgccc ccgcccgtga tgccgaaatg gttgccgatg
    32701 tgcgcgtgct gggggcaatc ggcgtagtcg aaacaactcg tccggtgaat atggcggcgc
    32761 tgcaaaaatt ctttgtcgaa cagggggtat ggatccggcc ttttggcaaa ctgatttacc
    32821 tgatgccgcc ctatattatt ctcccgcaac agttgcagcg tctgaccgca gcggttaacc
    32881 gtgcggtgca ggatgaaaca tttttttgcc aataacggga agtcagcgtg tgggtttctg
    32941 gctacacttt ctgcaaacta gaaaggaggg ttcatgaaac tcatcagtaa tgatctgcgc
    33001 gatggcgata agctgccgca tcgtcatgtc tttaacggca tgggttacga tggcgataat
    33061 atttcaccgc atctggcgtg ggatgatgtt cctgcgggaa cgaaaagttt tgttgtcacc
    33121 tgctacgacc cggatgcgcc aaccggctcc ggctggtggc actgggtagt tgttaattta
    33181 cccgctgata cccgcgtatt accgcaaggg tttggctctg gtctggtagc aatgccagac
    33241 ggcgttttgc agacgcgtac cgattttggt aaaaccggat acgatggcgc agcgccgccg
    33301 aaaggcgaaa cccatcgcta catttttacc gttcacgcgc tggatgtaga gcatattgat
    33361 gtcgatgaag gagccagcgg cgcgatggtc gggtttaacg ttcatttcca ctctctggca
    33421 agcgcatcga ttaccgcgat gtttagttaa tcactctgcc agatggcgca atgctatctg
    33481 gtatcactta aaggtattaa aaacaacttt ttgtcttttt accttcccgt ttcgctcaag
    33541 ttagtataaa aaagcaggct tcaacggatt catttttcta tttcatagcc cggagcaacc
    33601 tgtgaacaca ttttcagttt cccgtctggc gctggcattg gcttttagcg tgacgctgac
    33661 cgcctgtagc tcaacaccac ccgatcaacg tccttctgat caaactgcgc ctggtacctc
    33721 ttctcgcccg attctgtcgg caaaagaagc gcagaatttc gatgctcaac actattttgc
    33781 atccctgaca ccaggtgctg cagcgtggaa tccttccccg attaccctgc ctgcgcaacc
    33841 tgactttgtt gtcggcccgg cgggcactca aggtgtaacg cataccacga ttcaggcggc
    33901 ggtagatgcg gcaattatca agcgtaccaa caagcgccag tatattgccg tgatgcctgg
    33961 tgagtatcag ggaacggtgt atgtccctgc tgctccgggt ggaattactc tgtacggtac
    34021 aggtgaaaaa ccgattgatg tgaagattgg gctttccctt gatgggggca tgagccctgc
    34081 cgactggcgt cacgacgtca acccgcgcgg caaatatatg ccaggtaaac cagcgtggta
    34141 tatgtacgat agctgccaga gcaaacgcag cgacagtatc ggtgttctct gctctgcggt
    34201 cttctggtca caaaacaatg gcctgcaact gcaaaacctg accatcgaaa acacgctggg
    34261 cgatagcgta gatgcaggta accatccggc ggtggcactg cgtactgatg gtgaccaggt
    34321 acagattaac aacgttaaca ttctcggttg tcagaacacc ttctttgtca ccaacagcgg
    34381 tgtgcagaac cgtctggaaa cgaatcgtca gccgcgtacg ctggtgacca acagctacat
    34441 tgaaggggat gtggatatcg tttctggtcg cggcgcagtg gtgttcgata acaccgaatt
    34501 ccgcgtggtg aactcacgta ctcagcaaga agcgtatgtg tttgcaccgg ctacgctgtc
    34561 caacatttac tacggtttcc tcgccgtaaa cagccgtttc aatgctttcg gtgatggtgt
    34621 ggcgcaactg ggccgctcgc tggatgttga tgccaatacc aacggtcagg tggtgatccg
    34681 tgatagcgcc atcaacgaag gttttaacac ggctaaaccg tgggccgatg cggtgatctc
    34741 taatcgtccg tttgcgggta ataccggcag cgtagatgat aacgacgaaa tacagcgcaa
    34801 tctgaatgac actaactaca accgcatgtg ggaatacaat aaccgcggcg tgggtagtaa
    34861 agtggttgca gaggcgaaga agtaagagca attaactatt tgccggatgc ggcgtaaacg
    34921 ccttatccgg cctacggttc gatgcgattt gtaggtcgga taagatgcgc aagcatcgca
    34981 tccgacaata agtgccgaat gcgacctaca ttcacatggc gctttttaca tctgacggtt
    35041 tttattgaag ttaatcaaac tacccgcctt gataatctcg cgctcttcag cagtcagact
    35101 ttccatatag agcgtaattt ccgttaccgg cgcatcttca tggatcacat aacctttaaa
    35161 cgtcgtaccc ggattatcca gcgccgcttt aatgccaggg atgtaaatgt aatcccccac
    35221 ttcaaaggtt ggcacttccg ccatctgcag cggtaacatc ccccagttga tgacgttaga
    35281 acggtagcgt ttagtcgcgt actcttcggc aatattcgcc agaccgccaa tcacacgctg
    35341 gcagctcgcc gcctgttcac gcgcagaacc atcgcctggt ttcaccgcat ataccatgct
    35401 gccaatttca gtttgcagcg gatcaatatg ctcctgacca gcaatctgct taatgcgcgc
    35461 aaacacctct gtcagctcgc tgacattccc cgccagacgc tgattttcca gctcagccgt
    35521 cgctttactt ctgccaacat aaccgggatc gcggcgtgac agggtaaact ccgccagacc
    35581 aatcggattt gaacgataag aagaggtttc accggaagga atcagttcgt cggtggtggt
    35641 cacttcgtcg aggatcttcg agcacacttt caggacgata ttgtcagtca gcgcacccaa
    35701 ttccggccag tctttaatgt tcggtccgta aatcagcggt tgctgagttg cccctttcac
    35761 aaagccctga taaacacggt ttttatacgg cgttacatcg aaggcgtact ccggcacgtt
    35821 gtcccagcaa tcaagttcgc tggcagaggt taaatagcca ccgtttgccg cagtcgcagc
    35881 gatagaacga gcgtccatca acgccaccgc tgacatctgc ccattagctg gcttagagcc
    35941 ttcgcggttc gggaagttac gcgtggtgtg gcgaatactt aatccgttgt tgattggcgt
    36001 atcgcccgcg ccaaagcatg ggccgcagaa cgcggttctg atgattgcgc ctgcgccaat
    36061 caaatctgct accacacctt ttttggctaa atccataaac accggctgtg atgacgggta
    36121 aaccgccagc gagaaggtgt cattgccaca ggattgaccg cgcagtgcat tcgccgccgc
    36181 gatgacgttt tcgtagttac cgccagaaca gcccgcgata atcccctgct gcactttcag
    36241 gcgaccattt tccactttat ccagcagcga gagtttggct ttaccgtgcg ccacgcgttc
    36301 ggactcaatt tcaatctcac gcagaatgtc ggtcaggttc tggttcagtg tgtcgatttc
    36361 atacacgttg ctcgggtgga acggcagcgc aatcattggt ttgatggcgc ttaaatcaac
    36421 gctgatgcag ccatcgtagt acgccatcgg ttgagggtta agctggcagt aatcctggcc
    36481 gcgaccgtgc agcgccagcc agttatgcac ttcttcatca gtttgccaga cggagcttaa
    36541 acaggtggtt tccgtggtca tcacgtcaac gctgttacgg aaatcggtag agagcgcgct
    36601 aacgcccggt ccaacgaact ccatgacttt gtttttgacg taaccgtttt tgaacaccgc
    36661 gccaatgata gccagcgcca catcctgtgg ccccacatac ggcgcaggtt ttccggtcag
    36721 atgcaccgca accacgcccg gatagtcgat atcccaggtg tcattaagca gctgttttac
    36781 caactcaccg ccgccctcac cgactgccat tgtccctaat gcaccgtaac gggtgtggct
    36841 gtctgacccg aggatcattt tgcccccgcc tgccatcatc tcacgcatat attgatggat
    36901 gatcgcaata tgcggaggca caaaaatacc gccataacgc tgagctgccg ataaaccaaa
    36961 aacatggtca tcaccgttaa tggtgccgcc gacggcgcag agtgagttat ggcagttggt
    37021 cagcacatac ggcagcggga aacgttccat tccggacgct ttagcggtct gtacaatacc
    37081 aacaaaggta atatcgtgag aggctaatga atcaaactta attttaagtt tatccatatt
    37141 tccggacgta ttatgagaag agagaataga ccaggcaata gtgccttttt tggcttcttc
    37201 ttttttaatt tcgccggtga aatgttcttc ggcaattatt tcgttattac tggcgagaa
//
LOCUS       DAJTNN010000016        36826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTNN010000016 DAJTNN010000000
VERSION     DAJTNN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.016"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            584..670
                     /gene="leuL"
                     /locus_tag="OO150_000757"
     CDS             584..670
                     /gene="leuL"
                     /locus_tag="OO150_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5514998.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            763..2334
                     /gene="leuA"
                     /locus_tag="OO150_000758"
     CDS             763..2334
                     /gene="leuA"
                     /locus_tag="OO150_000758"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5514999.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2334..3425
                     /gene="leuB"
                     /locus_tag="OO150_000759"
     CDS             2334..3425
                     /gene="leuB"
                     /locus_tag="OO150_000759"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5515000.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3428..4828
                     /gene="leuC"
                     /locus_tag="OO150_000760"
     CDS             3428..4828
                     /gene="leuC"
                     /locus_tag="OO150_000760"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5515001.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGQVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4839..5444
                     /gene="leuD"
                     /locus_tag="OO150_000761"
     CDS             4839..5444
                     /gene="leuD"
                     /locus_tag="OO150_000761"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5515002.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5493..6575)
                     /locus_tag="OO150_000762"
     CDS             complement(5493..6575)
                     /locus_tag="OO150_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5515003.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYEDNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(6676..6807)
                     /gene="sgrT"
                     /locus_tag="OO150_000763"
     CDS             complement(6676..6807)
                     /gene="sgrT"
                     /locus_tag="OO150_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5515004.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6896..8551
                     /gene="sgrR"
                     /locus_tag="OO150_000764"
     CDS             6896..8551
                     /gene="sgrR"
                     /locus_tag="OO150_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5515005.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSTLRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVNYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8585..8684
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8715..9698
                     /gene="thiB"
                     /locus_tag="OO150_000765"
     CDS             8715..9698
                     /gene="thiB"
                     /locus_tag="OO150_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5515006.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            9674..11284
                     /gene="thiP"
                     /locus_tag="OO150_000766"
     CDS             9674..11284
                     /gene="thiP"
                     /locus_tag="OO150_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5515007.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11268..11966
                     /gene="thiQ"
                     /locus_tag="OO150_000767"
     CDS             11268..11966
                     /gene="thiQ"
                     /locus_tag="OO150_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5515008.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(12079..12843)
                     /locus_tag="OO150_000768"
     CDS             complement(12079..12843)
                     /locus_tag="OO150_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5515009.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12929..13807)
                     /gene="araC"
                     /locus_tag="OO150_000769"
     CDS             complement(12929..13807)
                     /gene="araC"
                     /locus_tag="OO150_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5515010.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14146..15846
                     /gene="araB"
                     /locus_tag="OO150_000770"
     CDS             14146..15846
                     /gene="araB"
                     /locus_tag="OO150_000770"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5515011.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQTQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15857..17359
                     /gene="araA"
                     /locus_tag="OO150_000771"
     CDS             15857..17359
                     /gene="araA"
                     /locus_tag="OO150_000771"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5515012.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQHLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17559..18254
                     /gene="araD"
                     /locus_tag="OO150_000772"
     CDS             17559..18254
                     /gene="araD"
                     /locus_tag="OO150_000772"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5515013.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18329..20680
                     /gene="polB"
                     /locus_tag="OO150_000773"
     CDS             18329..20680
                     /gene="polB"
                     /locus_tag="OO150_000773"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5515014.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGSENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20844..23750
                     /gene="rapA"
                     /locus_tag="OO150_000774"
     CDS             20844..23750
                     /gene="rapA"
                     /locus_tag="OO150_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5515015.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHMPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23762..24421
                     /gene="rluA"
                     /locus_tag="OO150_000775"
     CDS             23762..24421
                     /gene="rluA"
                     /locus_tag="OO150_000775"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5515016.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24538..25353)
                     /gene="djlA"
                     /locus_tag="OO150_000776"
     CDS             complement(24538..25353)
                     /gene="djlA"
                     /locus_tag="OO150_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5515017.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25608..27962
                     /gene="lptD"
                     /locus_tag="OO150_000777"
     CDS             25608..27962
                     /gene="lptD"
                     /locus_tag="OO150_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5515018.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28015..29301
                     /gene="surA"
                     /locus_tag="OO150_000778"
     CDS             28015..29301
                     /gene="surA"
                     /locus_tag="OO150_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5515019.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29301..30290
                     /gene="pdxA"
                     /locus_tag="OO150_000779"
     CDS             29301..30290
                     /gene="pdxA"
                     /locus_tag="OO150_000779"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5515020.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMILNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30287..31108
                     /gene="rsmA"
                     /locus_tag="OO150_000780"
     CDS             30287..31108
                     /gene="rsmA"
                     /locus_tag="OO150_000780"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5515021.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            31111..31488
                     /gene="apaG"
                     /locus_tag="OO150_000781"
     CDS             31111..31488
                     /gene="apaG"
                     /locus_tag="OO150_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5515022.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31495..32337
                     /gene="apaH"
                     /locus_tag="OO150_000782"
     CDS             31495..32337
                     /gene="apaH"
                     /locus_tag="OO150_000782"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5515023.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32415..32894)
                     /gene="folA"
                     /locus_tag="OO150_000783"
     CDS             complement(32415..32894)
                     /gene="folA"
                     /locus_tag="OO150_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5515024.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33086..34948)
                     /gene="kefC"
                     /locus_tag="OO150_000784"
     CDS             complement(33086..34948)
                     /gene="kefC"
                     /locus_tag="OO150_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5515025.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34941..35471)
                     /gene="kefF"
                     /locus_tag="OO150_000785"
     CDS             complement(34941..35471)
                     /gene="kefF"
                     /locus_tag="OO150_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5515026.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35579..36826)
                     /locus_tag="OO150_000786"
                     /pseudo
     CDS             complement(35579..36826)
                     /locus_tag="OO150_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8959 a        10085 c         9687 g         8095 t
ORIGIN      
        1 catgcatata aaataagaaa aagcaaaatg agtaaaattc ggaaaacata aagatacggg
       61 ttctgataaa aacagaatcc attgccgtgg aatgagtcat ttaccacagc ataataatcc
      121 ataatgtttt tgttgcgaaa acaatctaat cataactacc gcgaatactc aatcatctac
      181 aaaatggatt aaatgtgctt atttaataat taatttatat atttaatgca ttaattatta
      241 acattaatgg atcaataata ttcaccatat caatatcaaa aaaaattcgc aaaacatata
      301 attcaataca aatcatcagg ataggttttg caacgcgtgc attttgtccc ctttttcctc
      361 gttgattaga tgcaaaaatt tattctgaaa tatgtcaacc tatgaaaagt gtcggtcgtt
      421 aaacagaaat taatatcgct tgctttaacc accgcagcac aattagctaa ttttacggat
      481 gcagaactcg cgctggcggg acgtttttat tgcgtcaggg ttgacatccg tttttgtatc
      541 cagtaactct aaaagcatat cgcattcatc tggagctgat ttaatgactc acatcgttcg
      601 ctttatcggt ctactactac taaacgcatc ttctttgcgc ggtagacgag tgagcggcat
      661 ccagcattaa gccagcacgc agtcaaacaa aaaacccgcg ccattgcgcg ggttttttta
      721 tgcccgaagc gaggcgctct aaaagagaca aggacccaaa ccatgagcca gcaagtcatt
      781 attttcgata ccacattgcg cgacggtgaa caggcgttac aggcaagctt gagtgtgaaa
      841 gaaaaactgc aaattgcgct ggcccttgag cgtatgggtg ttgacgtgat ggaagtcggt
      901 ttccccgtct cttcgccggg cgattttgaa tcagtgcaaa ccatcgcccg ccaggttaaa
      961 aacagccgcg tatgcgcgtt agctcgctgc gtggagaaag atatcgacgt ggcggccgaa
     1021 tccctgaaag tcgccgaagc cttccgtatt cataccttta ttgccacttc gccaatgcac
     1081 atcgccacca agctgcgcag cacgctggat gaagtgatcg aacgcgctat ctatatggtg
     1141 aaacgcgccc gtaattacac cgatgatgtt gaattttctt gcgaagatgc cgggcgtacc
     1201 cccattgccg atctggcgcg agtggtcgaa gcggcgatta atgccggtgc caccaccatc
     1261 aacattccgg acaccgtggg ctacaccatg ccgtttgagt tcgccggaat catcagcggt
     1321 ctgtatgaac gcgtacctaa catcgacaaa gccattattt ccgtacatac ccacgacgat
     1381 ttaggcctgg cggtcggcaa ctcactggcg gcggtacatg ccggtgcacg ccaggtggaa
     1441 ggtgcaatga acgggatcgg cgagcgtgcc ggtaactgtt cgctggaaga agtcatcatg
     1501 gcgattaaag ttcgtaagga tattctcaac gtccacaccg ccattaatca ccaggagata
     1561 tggcgcacca gccagttagt tagccagatt tgtaatatgc cgatcccggc aaacaaagcc
     1621 attgttggca gcggcgcatt cgcccactcc tccggtatcc accaggatgg cgtgctgaaa
     1681 aaccgcgaaa actacgaaat catgacacca gaatctattg gtctgaacca aatccagctg
     1741 aatctgacct ctcgttcggg gcgtgcggcg gtgaaacatc gcatggatga gatggggtat
     1801 aaagaaagtg aatataattt agacaatttg tacgatgctt tcctgaagct ggcggacaaa
     1861 aaaggtcagg tgtttgatta cgatctggag gcgctggcct tcatcggtaa gcagcaagaa
     1921 gagccggagc atttccgtct ggattacttc agcgtgcagt ctggctctaa cgatatcgcc
     1981 accgccgccg tcaaactggc ctgtggcgaa gaagtcaaag cagaagccgc caacggtaac
     2041 ggtccggtcg atgccgtcta tcaggcaatt aaccgcatca ctgactataa cgtcgaactg
     2101 gtgaaataca gcctgaccgc caaaggtcac ggtaaagatg cgctgggtca ggtggatatt
     2161 gtcgccaact acaacggtcg ccgcttccac ggcgtcggcc tggccaccga tattgtcgag
     2221 tcctccgcca aagccatggt gcacgtactg aacaatatct ggcgtgccgc agaagtcgaa
     2281 aaagagttgc aacgcaaagc tcaacacaac gaaaacaaca aggaaaccgt gtgatgtcga
     2341 agaattacca tattgccgta ttgccggggg acggtattgg tccggaagtg atgacccagg
     2401 cgctgaaagt gctggatgcc gtgcgcaacc gctttgcgat gcgcatcacc accagccatt
     2461 acgatgtagg cggcgcagcc attgataacc acgggcaacc actgccgcct gcgacggttg
     2521 aaggttgtga gcaagccgat gccgtgctgt ttggctcggt aggcggtccg aaatgggaac
     2581 atttaccacc agaccagcaa ccagaacgcg gcgcgctgtt gcctttgcgt aagcacttca
     2641 aattattcag caacctgcgt ccggcaaaac tgtatcaggg gctggaagca ttctgtccgc
     2701 tgcgtgctga cattgccgct aacggcttcg acatcctgtg cgtgcgcgaa ctgaccggcg
     2761 gcatctattt cggtcagcca aaaggccgcg aaggtagcgg acagtatgaa aaagcgtttg
     2821 ataccgaggt gtatcaccgt tttgagatcg aacgtatcgc ccgcatcgcg tttgaatctg
     2881 cccgcaagcg tcgccacaaa gtcacctcaa tcgacaaagc caacgtgctg caatcctcta
     2941 ttttatggcg ggagatcgtt aacgagatcg ccacggaata cccggatgtc gaactggcgc
     3001 atatgtacat cgacaacgcc accatgcagc tgattaaaga tccatcacag tttgacgtcc
     3061 tgctgtgctc caacctgttt ggcgacattc tgtctgacga gtgcgcaatg atcactggct
     3121 cgatggggat gttgccttcc gccagcctga acgagcaagg ttttggtctg tatgaaccgg
     3181 caggcggctc agcaccagat atcacaggca aaaacatcgc caacccgatt gcgcaaattc
     3241 tgtcgctggc actgctgctg cgctacagcc tggatgccga tgatgcggct tgcgccattg
     3301 aacgcgccat taaccgcgca ttagaagaag gcattcgcac cggggattta gcccgtggcg
     3361 ctgccgccgt tagtaccgat gaaatgggcg atatcattgc ccgctatgtg gcagaagggg
     3421 tgtaatcatg gctaaaacgt tatacgaaaa attattcgac gctcacgtag tgtacgaagc
     3481 cgaaaacgaa accccgctgt tatatatcga ccgccacctg gtgcatgaag tgacctcacc
     3541 gcaggcgttc gatggtctgc gcgcccacgg tcgcccggta cgtcagccgg gcaaaacctt
     3601 cgccactatg gatcacaacg tctctaccca gacgaaagac attaatgcct gtggtgaaat
     3661 ggcgcgcatc cagatgcagg agctgattaa aaactgcaaa gaatttggcg tcgaactgta
     3721 tgacctgaat cacccgtatc aggggatcgt ccacgtaatg gggccggaac agggcgtcac
     3781 cttgccgggg atgaccattg tctgcggcga ctcgcatacc gccacccacg gcgcgtttgg
     3841 cgcactggcc tttggtatcg gcacttccga agttgaacac gtactggcaa cgcaaaccct
     3901 gaaacagggc cgcgcaaaaa ccatgaaaat tgaagtccag ggcaaagccg cgccgggcat
     3961 taccgcaaaa gatatcgtgc tggcaattat cggtaaaacc ggtagcgcag gcggcaccgg
     4021 gcatgtggtg gagttttgcg gcgaagcaat ccgtgattta agcatggaag gtcgtatgac
     4081 cctgtgcaat atggcaatcg aaatgggcgc aaaagccggt ctggttgcac cggacgaaac
     4141 cacctttaac tatgtcaaag gccgtttgca tgcgccgaaa ggcaaagatt tcgacgacgc
     4201 cgttgcctac tggaaaaccc tgcaaaccga cgaaggcgca actttcgata ccgttgtcac
     4261 tctgcaagca gaagaaattt caccgcaggt cacctggggc accaatccag gccaggtgat
     4321 ttccgtgaac gacaatattc ccgatccggc ttcgtttgcc gatccggttg aacgcgcgtc
     4381 ggcagaaaaa gcgctggcct atatggggct gaaaccgggc attccgctga ccgaagtggc
     4441 tatcgacaaa gtgtttatcg gttcctgtac caactcgcgc attgaagatt tacgcgcggc
     4501 agcggagatc gccaaagggc gaaaagtcgc gccaggcgtg caggcactgg tggttcccgg
     4561 ctctggccag gtaaaagccc aggcggaagc ggaaggtctg gataaaatct ttattgaagc
     4621 cggttttgaa tggcgcttgc ctggctgctc aatgtgtctg gcgatgaaca acgaccgtct
     4681 gaatccgggc gaacgttgtg catccaccag caaccgtaac tttgaaggcc gccaggggcg
     4741 cggcgggcgc acgcatctgg tcagcccggc aatggctgcc gctgctgctg tgaccggaca
     4801 tttcgccgac attcgcaaca ttaaataagg agcagaccat ggcagagaaa tttatcaaac
     4861 acacaggcct ggtggttccg ctggatgccg ccaatgtcga taccgatgca atcatcccga
     4921 aacagttttt gcagaaagtg acccgtacgg gttttggcgc gcatctgttt aacgactggc
     4981 gttttctcga tgaaaaaggc caacagccaa acccggactt cgtgctgaac ttcccgcagt
     5041 atcagggcgc ttccattttg ctggcacgag aaaacttcgg ctgtggctcc tcgcgtgagc
     5101 acgcgccctg ggcattgacc gactacggtt ttaaagtggt gattgcgccg agttttgctg
     5161 acatcttcta cggcaatagc tttaacaacc agctgctgcc ggtgaaatta agcgatgcag
     5221 aagtggacga actgtttgcg ctggtgaaag ccaatccggg gatccatttc gacgtggatc
     5281 tggaagcgca agaggtgaaa gcgggagaga aaacctatcg ctttaccatc gatgccttcc
     5341 gccgccactg catgatgaac ggtctggaca gtattgggct taccttgcag cacgacgacg
     5401 ccattgccgc ttatgaagca aaacaacctg cgtttatgaa ttaatcccct tgcccggtca
     5461 aatgaccggg ctttccgcta tcgtccacgt catcaaacgt ctttaacctt tgcggttaaa
     5521 aataatgcga caacagaaat aaccgcaatt acccagtaga cagcaaagtg cccccaactt
     5581 tgtgcaattg ctccctgaat aacgccagcc agaattaccc cggtagaaat actgttagta
     5641 aataaggtgg tagctgcccc cgctcttcca ggcattaaat cctgaaacca tagcatccca
     5701 atacccgcaa caatgccgat aaatacagcg ttaaaaagtt gcagcgtcat caacgccata
     5761 cggctatgaa agaaaatcaa tccggtgtaa aacagtactc ctgccgccac tgctatgacc
     5821 atcattcgcc gcttaccata acgtttgaca tagtagccag ccagaatcat tgctggtatt
     5881 tccagtccag ctgccgtccc catcaggaaa cccgccagtt tgtctggcaa tcctaactcg
     5941 ctactgatcc acaacggcat atcaataatg tacatggtgt tgcaggtcca cattaacgtc
     6001 gaggcgacaa ataacatccg tacgttacta tcctgccagc caccttgcat tgataaagca
     6061 ttttccgacg gcagttctac ccgcgccaca gacggaagca taaatgcaat caataccaga
     6121 ctgagtgtga atatcccggc ggcaatcgaa aacatcacca cttctcgcgc cgagttatcc
     6181 tcatattccc gcgccagagc aaataactgt ggcattgccg tattggccag agatgccaga
     6241 agcacaccac aggtgataag cgtcagataa tgacgattaa atgcaaacaa tggcgcattg
     6301 ccgatagcca tcaaacagca aaatataatc agttttcgcc gatcgccctg actgtcagaa
     6361 cgttttgcca accagaggct tacgccgatc ccagcaatag tattcaccgt ataaaagagt
     6421 ccgatccaga aaggttgcgc gccaacctca cgactcagaa ataagctcaa tgtaggagcc
     6481 tgtagcgccc cggccacccc catcataaaa gcgaccagca taaatgccgc gtaaacaccg
     6541 ttcatacggc gagccatcgt cattatccag atcatacgtt ccctttttag cgcggcgaga
     6601 ataaaaaaac cagcaggtat aatctgctgg cgggtgattt tacaccaata ctcagtcaca
     6661 catgatgcag gcaagtcaac tttcagaatt gcggtcatcc cactgcatca gtccttcaag
     6721 catggttaat cgttgtggga cgcttaacca acgcaaccag cacaacttcg ctgtcgcggt
     6781 aaaatagtgc tgataaaact gacgcatggg gcacctcctt gcttcatcgt taggaattat
     6841 tgacttaata tagggaaaat aaaattgctg tcttttgcgc aggagttccc cttttatgcc
     6901 atctgctcgt ctgcaacaac agttcatccg cctgtggcaa tgctgcgagg gtaaatcgca
     6961 ggacacaacg ctcaacgaac tggcagcgtt attgagctgc tcgcgtcgtc atatgcgtac
     7021 cctgctcaac accatgcagg atcgcggctg gctgacgtgg gaagcggaag tcgggcgcgg
     7081 taaacgctcg cgtctgacat tcctctatac cgggctggcg cttcagcaac agcgggcgga
     7141 agacctgctg gagcaggatc gtatcgacca actagtgcag ttggttggcg acaaagcgac
     7201 tgtgcggcaa atgctggttt ctcatctggg ccgcagcttc cgccaggggc ggcacatcct
     7261 gcgcgtgctc tactatcgtc cgttgcgtaa tctgctacct ggcagcacat tgcgccgttc
     7321 cgaaacccat attgcccggc aaatcttcag ttcgctaacg cgcataaatg aggaaaatgg
     7381 ggaactggaa gcagacatcg cccaccactg gcagcaaata tcaccgcttc actggcgttt
     7441 ctttttgcgt ccaggagtcc attttcatca tggtcgtgaa ctggaaatgg acgacgtgat
     7501 cgcctcttta aaacgaatca atacgctgcc gctctattcg catattgcgg acattgtgtc
     7561 gccgacgccc tggacgctgg atatccatct cacgcaaccg gaccgctggt taccgttact
     7621 gctggggcaa gttccggcga tgatcctgcc gcgcgaatgg gaaaccctca gtaactttgc
     7681 cagccatccc atcggcaccg gtccgtatgc ggtgatccgc aacaccacca atcaactgaa
     7741 aattcaggca ttcgatgact tcttcggtta ccgggcatta atcgatgaag ttaacgtctg
     7801 ggttctgccg gaaattgccg acgagccagc tggaggtctg atgctaaaag gtccacaggg
     7861 tgaggaaaaa gagattgaaa gccgcctgga ggaaggttgc tactatttac tattcgacag
     7921 ccgcacccat cgcggagcga atcagcaagt cagggactgg gtaaactatg tgctttctcc
     7981 aactaatctg gtctatttcg ctgaggaaca gtaccagcaa ctgtggttcc cggcttatgg
     8041 actgctcccc cgttggcacc atgcccgcac cataaagagc gaaaaaccgg ctggcctgga
     8101 aagcctcacc ctgacctttt atcaggatca cagtgagcat cgggtgattg ccgggatcat
     8161 gcagcagatt ctggcaagtc accaggtcac gctggaaatc aaagagatca gctacgatca
     8221 gtggcatgaa ggagagatcg agagcgatat ctggcttaat agcgccaact ttacgctgcc
     8281 gctggatttt tcgctgttcg cgcacttgtg cgaggtgccg ctgctccaac actgtattcc
     8341 gatcgactgg caagccgacg ccgcccgctg gcgcaatggc gaaatgaacc tggcgaactg
     8401 gtgccagcaa ctggtcgcca gtaaagcaat ggtgccgctt atccaccact ggctgatcat
     8461 tcagggacaa cgcagtatgc gcggcctgcg tatgaatacc ctcggctggt tcgattttaa
     8521 atcagcgtgg tttgcgccgc cggatccatg attgctggta gataacaaaa tcactacact
     8581 aacgccgttc tcaacggggt gccacgcaaa cgcgtgcgct gagaaaatac ccgtcgaacc
     8641 tgatccggat aacgccggcg aagggatttg aggctccttc tcaagtcctt tgccactctt
     8701 ttttgaggtg caaagtgtta aaaaaatgtc ttcccctgct gttgctgtgc acagcgcccg
     8761 ttttcgctaa acccgttctg actgtttata cctacgattc cttcgccgcc gactgggggc
     8821 ctggtccaaa gatcaaaaaa gcttttgaag ccgactgtaa ctgcgaactg aaactggtgg
     8881 cgctggaaga tggcgtttcg cttctcaacc gtctacggat ggaaggcaaa aatagtaaag
     8941 ccgatgtggt gctggggctg gataacaatc tgttagacgc cgccagtaaa accggactat
     9001 ttgccaaaag cggtgtggca gcggatgccg ttaacgttcc cggcggctgg aataatgaca
     9061 ctttcgtccc gtttgattac ggctatttcg ccttcgtcta tgacaagaac aaactgaaaa
     9121 acccgccgca aagcctgaaa gaattggttg agagcgatca aaactggcgg gtgatttatc
     9181 aggatccgcg caccagtacg ccggggctgg gcctgctgtt gtggatgcaa aaagtctatg
     9241 gcgatgacgc cccacaagcc tggcaaaaac tggcgaagaa aaccgtcacg gtcaccaaag
     9301 gctggagcga agcctacggc ctgtttttaa aaggtgaaag cgatctggta ctgagttaca
     9361 ccacctctcc ggcttatcac attctcgaag agaaaaaaga taactacgcc gccgcgaact
     9421 ttagcgaagg tcactatctg caggtggaag tcgccgcccg caccgctgcc agcaagcagc
     9481 cggagctggc gcaaaaattc ctccagttta tggtttctcc ggcttttcag aatgcgatcc
     9541 caaccggtaa ctggatgtat ccggtggcaa atgtcacgct gcctgccggg tttgaacaat
     9601 tgaccaaacc ggccaccacg ctggagttca caccagctga agtggcggca caacgtcagg
     9661 catggattag cggatggcaa cgcgccgtca gccgttaatt cccggctggt taattccagg
     9721 tgtaagcgcc gccacgctgg tggtagcggt tgcgctcgcg gcgtttctcg ccctgtggtg
     9781 gaacgcgccg cagggtaact gggtggcagt ctggcaggac agctacctgt ggcatgtggt
     9841 gcgcttctcc ttctggcagg cgtttctctc ggcgctactc tctgtcgtac ccgcgatatt
     9901 cctcgcccgc gccctctatc gcaggcgctt tccgggtcgg ctggcgctgt tgcgtctgtg
     9961 cgcaatgacc ttgatcctcc cggtgctggt tgctgttttc ggcattctta gcgtctatgg
    10021 tcgccagggc tggctggcat cgctctgcca atcgctcggt ctggagtgga ccttttcgcc
    10081 ctacggcctg caaggtattt tgctggcgca cgtatttttt aatctgccga tggcgagccg
    10141 cttattactc caggcactgg aaaacattcc cgacgaacaa cgtcagcttg ccgcccagct
    10201 tgggatgcgc ggctggcatt ttttccgctt cgtcgaatgg ccgtggttac ggcgacaaat
    10261 cccgccggtt gctgcactta tctttatgct ctgtttcgcc agcttcgcca ccgtgctatc
    10321 actgggcggc ggtccgcagg cgaccactat cgagctggct atttatcagg cgctgagtta
    10381 cgactacgat cctgcccgcg cggcgatgct ggcgctgatc cagatggtgt gttgccttgg
    10441 gctggtactg ttgagtcagc gattgagtaa ggccattgcg ccaggcacca cgctgctgca
    10501 aggctggcgc gacccggacg atcgtctgca tagccgcatt tgcgacacgg tgttaatagt
    10561 gctggcgttg ctgctgttgt tgccaccgtt gctggcggtg atcgtcgatg gggtaaatcg
    10621 ccagttgccg gaagtgctgg cacaaccggt gctgtggcag gcgctgtgga cctcgttgcg
    10681 tattgcgctg gcggcaggtg tattgtgcgt agtgctgacc atgatgctgc tatggagcag
    10741 tcgcgaactt cgggcacggc agaaaatgct ggcgggtcag gcgctggaga tgagcggcat
    10801 gttgattctc gccatgccgg ggattgtgct ggctaccggc ttctttttac tgctcaacaa
    10861 cactatcggc ctgcaacaat ctgctgacgg cattgtgatt ttcaccaatg cgttaatggc
    10921 gatcccttat gcgctgaaag tactggaaaa cccgatgcgc gatatcaccg cccgctacag
    10981 tatgttgtgt cagtcgctgg gcattgaagg ctggtcgcgc ttaaaagtgg tggagctgcg
    11041 cgccctgaaa cgtccactgg cgcaggcgct ggcctttgcc tgcgtgctgt cgattggtga
    11101 ttttggcgtg gtggcgttgt tcggtaacga tgatttccgt accctgccgt tttatctcta
    11161 ccagcaaatt ggctcctatc gcagccagga cggcgcggtc accgcgttaa ttctgctgct
    11221 actctgtttt ctgctgttta ccgtgattga aaaactaccg gggcgaaatg ttaaaactga
    11281 ctgatatcac ctggctttac caacatttgc cgatgcgttt tagcttaacg gtggaacgcg
    11341 gcgagcaggt ggcgatcctc gggccaagcg gcgcaggtaa aagtaccctg ctgaatttga
    11401 ttgccggttt tctgacgcca gccagcggtt cgctgactat cgatggcgtt gatcacacca
    11461 cgacgccgcc gtcacgccgt ccggtgtcga tgctgtttca ggagaacaac ctgttcagcc
    11521 acctgacggt cgcgcagaac atcgggctgg ggcttaatcc aggattgaag ctgaacgcgg
    11581 cacagcagga gaaaatgcac gctatcgccc gtcagatggg gattgataat ttaatggcgc
    11641 ggttaccggg cgagctttcc ggcggccagc gacagcgcgt ggcgttagcg cgttgtctgg
    11701 tgcgcgaaca gccgatttta ttgctcgatg aaccgttctc tgcgctcgat ccggcgttac
    11761 gtcaggagat gctgacgctg gtgagcacga gctgccagca gcaaaaaatg acgctattga
    11821 tggtgtcgca cagtgtggaa gatgcggcgc ggatcgccac acgctcggtg gtggtcgccg
    11881 acgggcgcat cgcctggcag ggtaagactg aagaattgtt gagcggtaag gcgagtgctt
    11941 cggcaatatt ggggattacg ggttagtatg cactgtcaga tgcggcgtga acaccttatc
    12001 cgacctacgg acagtgcgtt ttgtaggcct gataagaccg gtcagcgtcg catcaggcaa
    12061 cccgcacaag acaatcccct aaaccccaac cactttacgc aaaatatcga tatacaccgg
    12121 catcaacggg tggcgaatta acaccaccag cgccaccacg ccgatggcag aaatcaacgg
    12181 cgtcagccac aacaaacggc cgcgcgacaa ataatgactt aaacgatcgg ttgctttacc
    12241 gctgcgccat aaccgccagc acagccagcc accaacccac aaaaataccg ccgtcgccag
    12301 cagcaaccat ttaaactcgc cgctctgcat tccggctgga atatcgatcg ccgcgcccgc
    12361 cagaatccca ggcaggaagt aaaacggtgg ccacagcagg cagccgataa tattcggcgt
    12421 aatgaatttt gccaccggca gatccagcat tcccgccacc attggcacca gcggacgcgt
    12481 cgggccaaca aagcgaccga ccagaatggt gaacatgctg tgttgatgca acgcatgttc
    12541 agttttatcg agtaatgctt tgtttttctt cagaaatgac cagcgatgca acggcttttt
    12601 aaaacgccag cccagccaga aggagatcca gtcgcccagc agacaaccga caatccctgc
    12661 cagccaggcg tgccagaaac ttaactcgcc gctgccaatc agcgctccca gccccgccat
    12721 cagcaccgta ccgggtagaa tcaaaccgac cagcgccagc gactccagaa aggccaccaa
    12781 caccaccgcc atcaatgaat acacggtaga ttgggtaata aagtgttcca gcaatgcttg
    12841 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
    12901 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
    12961 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
    13021 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
    13081 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
    13141 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
    13201 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
    13261 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
    13321 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
    13381 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
    13441 ccgggcgaaa gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccaat
    13501 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
    13561 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
    13621 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
    13681 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
    13741 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
    13801 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
    13861 gacattgccg tcactgcgtc ttttactggc tcttctcgct aacccaaccg gtaaccccgc
    13921 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
    13981 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
    14041 attccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
    14101 tctctactgt ttctccatac ccgttttttt ggatggagtg aaacgatggc gattgcaatt
    14161 ggcctcgatt ttggcagtga ttctgtgcga gctttggcgg tggactgcgc cagcggtgaa
    14221 gagatcgcca ccagcgtaga gtggtatccc cgttggcaaa aagggcaatt ttgtgatgcc
    14281 ccgaataacc agttccgtca tcatccgcgt gactacattg agtcaatgga agcggcactg
    14341 aaaaccgtgc ttgcagagct tagcgtcgaa cagcgcgcag ctgtggtcgg gattggcgtt
    14401 gacagtaccg gctcgacgcc cgcaccgatt gatgccgacg gtaacgtgct ggcgctgcgc
    14461 ccggagtttg ccgaaaaccc aaacgcgatg ttcgtattgt ggaaagacca cactgcggtt
    14521 gaagaagcgg aagagattac ccgtttgtgc cacgcgccgg gcaacgttga ctactcccgc
    14581 tacattggtg gtatttattc cagcgaatgg ttctgggcaa aaatcctgca tgtgactcgc
    14641 caggacagcg ccgtggcgca atctgccgca tcgtggattg agctgtgcga ctgggtgcca
    14701 gctctgcttt ccggtaccac ccgcccgcag gatattcgtc gcggacgttg cagcgccggg
    14761 cataaatctc tgtggcacga aagctggggc ggcttgccgc cagccagttt ctttgatgag
    14821 ctggacccga tcctcaatcg ccatttgcct tccccgctgt tcactgacac ctggactgcc
    14881 gatattccgg tgggcacctt atgcccggaa tgggcgcagc gtctcggcct gcctgaaagc
    14941 gtggtgattt ccggcggcgc gtttgactgc catatgggcg cagttggcgc aggcgcacag
    15001 cctaacgcac tggtaaaagt tatcggtact tccacctgcg acattctgat tgccgacaaa
    15061 cagagcgttg gcgagcgggc agttaaaggt atttgcggtc aggttgatgg cagcgtggta
    15121 cctggattta tcggtctgga agcaggccaa tcggcgtttg gtgatatcta cgcctggttt
    15181 ggtcgcgtac tcggctggcc gctggaacag cttgccgccc agcatccgga actgaaagcg
    15241 caaatcaacg ccagccagaa acaactgctt ccggcgctga ccgaagcatg ggccaaaaat
    15301 ccgtctctgg atcacctgcc ggtggtgctc gactggttta acggccgccg cacaccgaac
    15361 gctaaccaac gcctgaaagg ggtgattacc gatcttaacc tcgctaccga cgctccgctg
    15421 ctgtttggcg gtttgattgc tgccaccgcc tttggcgcac gcgcaatcat ggagtgcttt
    15481 accgatcagg ggatcgccgt caataacgtg atggcgctgg gcggcatcgc gcggaaaaac
    15541 caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc cgctgcaaat tgttgcctct
    15601 gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg tcgccgcgaa agtgcacgca
    15661 gacatcccat cagcccagca aaaaatggcc agtgcggtag agaaaaccct gcaaccgcgc
    15721 agcgaacaga cacaacgctt tgaacagctt tatcgccgct atcagcaatg ggcgatgagc
    15781 gccgaacaac actatcttcc aacttccgcc ccggcacagg ctgcccaggc cgttccgact
    15841 ctataaggac acgataatga cgatttttga taattatgaa gtgtggtttg tcattggcag
    15901 ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc caacatgccg agcacgttgt
    15961 taatgcgctg aatacggaag cgaaactgcc ctgcaaactg gtgttgaaac cgctgggcac
    16021 cacgccggat gaaatcaccg ctatttgccg cgacgcgaat tacgacgatc gttgcgctgg
    16081 tctggtggtg tggctgcaca ccttctcccc ggccaaaatg tggatcaacg gcctgaccat
    16141 gctcaacaaa ccgttgctgc aattccacac ccagtttaac gcggcgctgc cgtgggacag
    16201 tatcgatatg gactttatga acctgaacca gactgcacat ggcggtcgcg agttcggctt
    16261 cattggcgcg cgtatgcgtc agcaacatgc cgtggttacc ggtcactggc aggataaaca
    16321 agcccatgag cgtatcggct cctggatgcg tcaggcggtc tctaaacagg atacccgtca
    16381 tctgaaagtc tgccgttttg gcgataacat gcgtgaagta gcggtcaccg atggtgataa
    16441 agttgccgca cagatcaagt tcggtttctc cgtcaatacc tgggcggttg gcgatctggt
    16501 gcaggtggtg aactccatca gcgacggcga tgttaacgcg ctggtcgatg agtacgaaag
    16561 ctgctacacc atgacgcctg ccacacaaat ccacggcgaa aaacgacaga acgtgctgga
    16621 agcggcgcgt attgagctgg ggatgaagcg tttcctggaa caaggtggct tccacgcgtt
    16681 caccaccacc tttgaagatt tgcacggtct gaaacagctt ccaggtctgg ccgtacagca
    16741 tctgatgcag cagggttacg gctttgcggg cgaaggcgac tggaaaaccg ccgccctgct
    16801 tcgcatcatg aaggtgatgt caaccggtct gcagggcggc acctccttta tggaggacta
    16861 cacctatcac ttcgagaaag gtaatgacct ggtgctcggc tcccatatgc tggaagtctg
    16921 cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt cagcatctcg gtattggtgg
    16981 taaggacgat cctgcccgac tgatcttcaa tacccaaacc ggtccagcga ttgtcgccag
    17041 cctgattgat ctcggcgatc gttaccgtct gctggttaac tgtatcgaca cggtgaaaac
    17101 accgcactcc ctgccgaaac tgccggtggc gaatgcgctg tggaaagcgc aaccggatct
    17161 gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg caccataccg tcttcagcca
    17221 tgcgctgaac ctcaacgata tgcgtcagtt cgccgagatg cacgacattg aaatcacggt
    17281 gattgataac gacacccgcc tgccagcgtt taaagacgca ctgcgctgga acgaagtgta
    17341 ttacggattt cgtcgctaag tagccacatc aggtatgtaa cgcctgatgc gacgctgacg
    17401 cgtcttatct ggcctacacg ctgcgatttt gtaggccgga taagcaaagc gcatccggca
    17461 ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct acgcgctgcg attttgtaga
    17521 ccggataagc gaagcgcatc cggcacgaag gagtcaacat gttagaagat ctcaaacgcc
    17581 aggtattaga agccaacctg gcgctgccaa aacacaacct ggtcacgctc acatggggca
    17641 acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt
    17701 acagcgtcat gaccgctgac gatatggtcg tggttagcat cgaaaccggt gaagtggttg
    17761 aaggtacgaa aaagccctcc tccgacacgc caactcaccg gctgctctat caggcattcc
    17821 cctccattgg cggcattgtg catacgcact cgcgccacgc caccatctgg gcgcagtcgg
    17881 gtcagtcgat tccagcaacc ggcaccaccc acgccgacta tttctacggc accattccct
    17941 gcacccgcaa aatgaccgac gcagaaatca acggcgaata tgagtgggaa accggtaacg
    18001 tcatcgtaga aacctttgaa aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg
    18061 tccattccca cggcccgttt gcatggggca aaaatgccga agatgcggtg cataacgcca
    18121 tcgtgctgga agaggtcgct tatatggaga tattctgccg tcagttagcg ccgcagttac
    18181 cggatatgca gcaaacgctg ctggataaac actatctgcg taagcatggc gcgaaggcat
    18241 attacgggca gtaatgactg tataaaacca cagccaatca aacgaaacca ggctatactc
    18301 aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca ggttttatct taacccgaca
    18361 ctggcgggac accccgcaag ggacagaagt ctccttctgg ctggcgacgg acaacgggcc
    18421 gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt attcccgccg atcaggttcc
    18481 ccgcgctcag catattttgc agggtgaaca aggctttcgc ctgacaccgc tggcgttaaa
    18541 ggattttcac cgccagccgg tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa
    18601 ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc
    18661 agaacgctat ctgatggagc ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca
    18721 caatggcact atcgttaatg cccgtctgaa accgcatccc gactatcgtc cgccgctcaa
    18781 gtgggtttct atagatattg aaaccacccg ccacggtgag ctgtactgca tcggcctgga
    18841 aggctgcggg cagcgcatcg tttatatgct ggggtcggag aatggcgacg cctcctcgct
    18901 tgatttcgaa ctggaatacg tcgccagccg cccgcagttg ctggaaaaac tcaacgcctg
    18961 gtttgccaac tacgatcctg atgtgatcat cggttggaac gtggtgcagt tcgatctgcg
    19021 aatgctgcaa aaacatgccg agcgttaccg tcttccgctg cgtcttgggc gcgataatag
    19081 cgagctggag tggcgcgagc acggctttaa aaacggcgtc ttttttgccc aggctaaagg
    19141 tcggctaatt atcgacggta tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt
    19201 ctcgctggaa actgtcgctc aggagctatt aggcgaagga aaatctatcg ataacccgtg
    19261 ggatcgaatg gacgaaattg accgccgttt cgccgaagat aaacctgcgc tggcaactta
    19321 taacctgaaa gattgcgagc tggtgacgca gatcttccac aaaactgaaa tcatgccatt
    19381 tttactcgaa cgggcaacgg tgaacggcct gccggtggac cgacacggcg gttcggtggc
    19441 ggcatttggt catctctatt ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct
    19501 cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg atggattcac ggccagggct
    19561 ttatgattca gtgctggtgc tggactataa aagcctgtac ccgtcgatca tccgcacctt
    19621 tctgattgat cccgtcgggc tggtggaagg catggcgcag cctgatccag agcacagtac
    19681 cgaaggtttt ctcgatgcct ggttctcgcg agaaaaacat tgcctgccgg agattgtgac
    19741 taacatctgg cacgggcgcg atgaagccaa acgccagggt aacaaaccgc tgtcgcaggc
    19801 gctgaaaatc atcatgaatg ccttttatgg cgtgctcggc accaccgcct gccgcttctt
    19861 cgatccgcgg ctggcatcgt cgatcaccat gcgtggtcat cagatcatgc ggcaaaccaa
    19921 agcgttgatt gaagcacagg gctacgacgt tatctacggc gataccgact caacgtttgt
    19981 ctggctgaaa ggcgcacatt cggaagaaga agcggcgaaa atcggtcgtg cactggtgca
    20041 gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa cggctgacca gcgcattaga
    20101 actggagtat gaaacccatt tctgccgttt tctgatgcca accattcgcg gagccgatac
    20161 cggcagtaaa aagcgctacg ccgggctgat tcaggagggc gataaacagc ggatggtgtt
    20221 taaagggctg gaaaccgtgc gcaccgactg gacgccgctg gcccagcagt ttcagcagga
    20281 gctatacctg cgcatcttcc gcaacgagcc atatcaggaa tatgtacgcg aaaccatcga
    20341 caaactgatg gcgggtgaac tggatgcgcg actggtttac cgtaaacgcc ttcgccgtcc
    20401 gctgagcgag tatcagcgta atgtgccgcc tcatgtacgc gccgctcgcc ttgccgatga
    20461 agaaaaccaa aagcgtggtc gccccttgca atatcagaat cgcggcacca ttaagtacgt
    20521 atggaccacc aacggcccgg agccgctgga ctaccaacgt tcaccactgg attacgaaca
    20581 ctatctgacc cgccagctac aacccgtggc ggagggaata ctccctttta ttgaggataa
    20641 ttttgctaca cttatgaccg ggcaacttgg gctattttga gcaaaaaaag agttcgccag
    20701 ataccatttt gatgcgtgac gaatgctttg ccatccagta ccatagcgcc ctttccattc
    20761 ctggacctga ataacaccac tacctcataa gcacggtagc gggtggttat tgcctgcaat
    20821 taaagatata gagccgaaca catatgcctt ttacacttgg tcaacgctgg atcagcgata
    20881 cagaaagcga attgggactt ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac
    20941 ttttcccatc tactggtgaa aaccgtctgt acgcacgcag tgattccccc gtgacccgcg
    21001 tgatgttcaa ccctggtgat accattacca gccatgacgg ctggcagatg caagtcgaag
    21061 aagtaaaaga agaaaatggc ttgctgacct atatcggtac tcgcctggat actgaagagt
    21121 ccggcgtagc cctgcgtgaa gttttccttg atagcaaact ggtgttcagc aaaccgcagg
    21181 accgcctgtt tgccgggcag attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc
    21241 gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca
    21301 gcctgatccc gcaccagctc aacatcgctc atgatgttgg ccgccgccac gcgccgcgcg
    21361 tcctgctggc tgacgaagtg ggtttaggga aaaccattga agccgggatg atcctgcatc
    21421 agcaactgct ctctggcgct gctgaacgtg tgctgattat cgtcccggaa accttacagc
    21481 atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg ctttgctctg tttgatgatg
    21541 agcgttatgc cgaagctcag cacgatgctt acaacccgtt cgacaccgaa cagctggtga
    21601 tttgctcgct ggattttgcc cgtcgtagca aacagcgcct ggaacatctc tgtgaagccg
    21661 aatgggacct gctggtggtc gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa
    21721 gccgtgaata tcaggccatt gaacaactgg cagagcacat gccgggcgtt ctgctgctga
    21781 ccgcgacccc ggaacagctg gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc
    21841 cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca gaaaaattat cgtccggttg
    21901 cggacgccgt tgccatgctg ctggcaggta acaaactgag caatgacgaa ctgaacatgc
    21961 tcggcgagat gatcggcgag caggatatcg agccgctgtt gcaggcagca aacagcgaca
    22021 gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat gctgatggat cgccacggca
    22081 ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa aggattcccg aaacgcgagc
    22141 tgcacaccat taagctgccg ctaccgacgc agtatcagac ggctattaaa gtctccggca
    22201 ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga tatgctctac ccggagcgta
    22261 tttatcagga atttgaaggt gataacgcca cctggtggaa cttcgatccg cgcgttgagt
    22321 ggctgatggg ctacctgacc agccatcgct ctcagaaagt gctggtgatc tgcgccaaag
    22381 ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg
    22441 tgttccacga aggtatgtcg attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag
    22501 aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg ttctgaagga cgtaacttcc
    22561 agttcgccag ccacatggtg atgtttgacc tgccattcaa cccggatcta ctggagcagc
    22621 gtattggtcg tctggatcgt atcggccagg cgcacgatat tcagatccat gtgccttatc
    22681 tggagaaaac cgctcagtcg gtgctggtgc gctggtatca cgaaggtctg gatgcatttg
    22741 agcacacctg cccgaccgga cgcactattt acgatagcgt atacaacgat ctgattaact
    22801 atctggcttc accggatcaa accgaaggct ttgacgatct gatcaaaaac tgccgcgagc
    22861 aacatgaagc gctgaaagca cagctggaac agggtcgtga ccgcctgctg gaaatccact
    22921 ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat tgaagagcag gatgacgata
    22981 ccaacctgat cgccttcgcc atgaacctgc tcgatattat cggtatcaat caggacgatc
    23041 gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat gctggtgccg gacttccctg
    23101 gcctgtcgga agatggcatc accatcacct ttgatcgtga agtggcgctg gcgcgtgaag
    23161 atgcacagtt tattacctgg gagcatccgc tgatccgcaa cggtctggat ctgatccttt
    23221 ctggcgatac cggtagcagc acgatttcac tgttaaaaaa caaagcgttg ccggtaggta
    23281 cgctgttggt ggaactgatt tatgtggttg aagcccaggc tccgaagcag ttgcagctca
    23341 accgcttcct gccaccgacg ccggtacgta tgctgctgga taaaaacggc aacaacctgg
    23401 cggcgcaggt agagtttgaa acctttaacc gccagcttaa cgcggttaac cgtcacaccg
    23461 gcagcaaact ggttaacgcc gtgcagcagg atgttcacgc tatccttcaa ctgggtgaag
    23521 cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc gcgtaacgaa gccgacgaaa
    23581 aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc agtgaacccg aacattcgtg
    23641 acgacgaact gaccgccatt gagagcaacc gtcagcaggt aatggaaagc ctggatcagg
    23701 caggttggcg tctggatgcc ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa
    23761 aatggggatg gaaaactaca atccaccgca ggaaccctgg ttggttatcc tgtatcagga
    23821 tgaccacatt atggtggtta acaagccgag cggtttgttg tctgtgccgg ggcgtctgga
    23881 agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt
    23941 gcatcgtctg gatatggcta ccagcggcgt gattgtagtg gcgctgacca aagccgcgga
    24001 gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa aagcagtatg tggcccgcgt
    24061 ctgggggcat ccatcccctg cggaaggtct ggtggatctg ccgctgattt gcgactggcc
    24121 aaaccgcccg aaacagaaag tctgttacga aacgggtaaa cctgcgcaga cggaatatga
    24181 agtggtggag tatgcggcgg ataacacggc aagagtggtg ttaaaaccga ttactgggcg
    24241 ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat ccgattctcg gcgatcgttt
    24301 ttatgcatca ccagaagcga gagcgatggc accgcgtttg ttgctacatg cagagatgtt
    24361 gacgattacc catccggcgt atggcaatag tatgacgttt aaagcgccag cggattttta
    24421 atagtcacgc tggcgcagtt cggtaggcgg ataaggcgtt cacgccgaat ccggcaattg
    24481 tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca ggcctacagt ttaaaactta
    24541 tttaaaccct ttctgctgct ttatcagctc atatgcctgc tgaatttcct gcgctttctg
    24601 cttcgccatc tccatcatct caggcggcaa acctttcgcc accagcttat cgggatggtg
    24661 ttcactcatc agcttacggt aggctcgttt aatggtggtc gcatcatccg tcggcttcac
    24721 acccagcaca ttacaggcat cttccagcgt cgggccacgc tgcgcttgct gccagttacc
    24781 accgccagat tgctgctgat aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa
    24841 aaactggtca aactgggcgc gggagatccc taattcttct gcaatgacat acagcaccgc
    24901 ccgttcattc gggtgcagtg aaccatcagc aaacgctgcc tgaatctgga tctccagaaa
    24961 catacgaatt aagtcaaaac gaccaaagca gacactgcga aactggcgca tcttttcgcg
    25021 cagcgggtaa ttgtctgatt ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc
    25081 gccatgaagg ttcattcggt ccatcagctg gctggcgata tgaatatcag cctccgtgac
    25141 gcgacctttg gatttggtta aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc
    25201 ctgacgctca cgctggttgg cgaaccacgc cattttacgg ctacgggctt tatcaaacat
    25261 atggccaatt aacaggccta acactacgcc ccaaaagccg ccgcccatca gtaaggccac
    25321 ggccacgcca atgatttttc cccaatactg catatattcc ccaaatcgac acacggatat
    25381 cagggctatc tcccacaata taaaggtgct tttaccgttt tccggctgca gtcaattgtg
    25441 ggacatcgcc tataatttgc attatcatac ctgtcattca atgccgtgcc taacaccact
    25501 gacgctattc ggacaggatt aacactagcg tagagatgac gagtacgtta gtctctgacc
    25561 gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta
    25621 tccccactct cctggccacc atgattgcca ccgcccttta tagtcaacag ggactggcag
    25681 ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct ctggtacagg
    25741 gcgataccaa tgacttaccc gtgactatca atgctgacca cgcgaaaggg gactacccgg
    25801 atgacgccgt gtttactggc agcgtggata tcatgcaggg taacagccgt ctgcaggccg
    25861 acgaagtgca gctccatcaa aaagaggcac caggacaacc ggagccggta cgaaccgttg
    25921 atgcgctcgg taatgtccat tacgacgata accaggtgat cctcaaaggg ccgaaaggct
    25981 gggcgaatct gaacaccaaa gataccaacg tctgggaagg tgattaccag atggtgggtc
    26041 gccagggccg cggtaaagcg gacctgatga aacaacgtgg tgaaaaccgc tataccattc
    26101 tggataacgg tagctttacc tcctgtctgc cgggttctga cacctggagc gtggtaggta
    26161 gcgaaattat tcatgaccgc gaagaacaag ttgcggagat ctggaacgcc cgctttaagg
    26221 tgggtccagt accgatcttt tatagcccct atttgcagtt gccggtgggt gacaaacgtc
    26281 gctctggttt cttgatcccg aacgccaagt acaccaccac caactacttt gagttctacc
    26341 tgccatatta ctggaacatc gcgccaaata tggatgccac catcacgccg cattatatgc
    26401 atcgtcgtgg caacatcatg tgggagaacg aattccgcta cctctcccag gcgggcgctg
    26461 gcttgatgga actggactat ctgccttcag ataaagtcta tgaagatgaa cacccgaacg
    26521 atgacagttc acgtcgttgg ttgttctact ggcaacactc cggggtcatg gatcaagtgt
    26581 ggcgtttcaa cgtagactac accaaggtca gcgatcctag ctacttcaat gatttcgata
    26641 acaagtacgg ttccagtact gacggctacg caacgcaaaa attcagcgtt ggctatgcgg
    26701 tgcaaaactt caatgccacc gtttcaacca agcagttcca ggtctttagc gagcagaaca
    26761 ccagtagcta ctcggcagag ccgcagttag acgttaacta ctaccagaat gatgttggtc
    26821 cgtttgatac gcgtatttac ggccaggcag tgcactttgt taacaccaga gacgacatgc
    26881 ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt gccgctctct aataactggg
    26941 gcagcatcaa taccgaagcg aagttgctgg caacccatta tcagcaaacc aatcttgact
    27001 ggtataactc cagaaacacg accaagctgg acgaatccgt taaccgcgtt atgccgcaat
    27061 tcaaagttga cggcaaaatg gtctttgaac gcgatatgga aatgctggct ccgggttata
    27121 cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca
    27181 tctataacta cgactcgtct ctactgcaat ctgactactc tggcctgttc cgggaccgga
    27241 cttacggcgg tcttgaccgt attgcctccg ctaaccaggt gacgaccggt gtcacatctc
    27301 gcatatatga tgatgctgcc gttgaacatt ttaatatttc cgttggtcaa atctactatt
    27361 tcacggagtc tcgcactggc gatgacaaca taacatggga gaatgacgac aaaacgggct
    27421 cactggtgtg ggcaggcgat acttactggc gtatctccga gcgttgggga ttgcgtggcg
    27481 ggattcagta cgatacacgt ctggataacg tagcgaccag taactccagc attgaatacc
    27541 gtcgggatga agaccgtctg gtacagctga attaccgtta cgccagcccg gaatatattc
    27601 aggctacgct gcctaagtac tattccactg ctgagcaata taagaatggt atttcgcagg
    27661 taggtgctgt cgccagctgg ccaattgccg atcgttggtc cattgttggg gcctactact
    27721 acgacaccaa tgcgaacaag caagccgact ctatgttagg tgtgcaatac agctcctgct
    27781 gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg ttgggataac gataaacaac
    27841 atgcggtata tgacaacgca atcggcttta acatcgaact tcgcggcctg agctccaact
    27901 acggtctggg tacgcaagag atgctgcgtt cgaacattct gccgtatcaa aacactttgt
    27961 gatttgttga tttaccacgt aatccgcagt gcggttaatt gaaatggaaa aagtatgaag
    28021 aactggaaaa cgctgcttct cggtatcgcc atgatcgcga ataccagttt cgctgccccc
    28081 caggtagtcg ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt
    28141 gatggattaa tgcagtcggt aaaactgaac gctgcgcagg caaggcagca gcttcctgat
    28201 gacgcgacgc tgcgccacca aatcatggaa cgtttgatca tggatcaaat catcctgcag
    28261 atggggcaga aaatgggagt gaaaatctcc gatgagcagc tggatcaggc gattgctaac
    28321 atcgcgaaac agaacaacat gacgctggat cagatgcgca gccgtctggc ttacgatggt
    28381 ctgaactaca acacctatcg taaccagatc cgcaaagaga tgattatctc tgaagtgcgt
    28441 aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg aagtcgaatc cctggcgcag
    28501 caggtgggta accaaaacga cgccagcact gagctgaacc tgagccacat cctgatcccg
    28561 ctgccggaaa acccgacttc tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt
    28621 gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg cgattgctca ttctgccgac
    28681 cagcaggcgc tgaacggcgg ccagatgggc tggggccgta ttcaggagtt gccgggtatc
    28741 ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc
    28801 gttggcttcc atattctgaa agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg
    28861 accgaagttc atgctcgcca tattctgctg aaaccgtcgc cgatcatgac tgacgaacag
    28921 gcccgtgtga aactggaaca gattgctgcc gatatcaaga gtggtaaaac gacttttgct
    28981 gccgcagcga aagagttctc tcaggatcca ggctctgcta accagggcgg tgatctcggc
    29041 tgggctacac cagatatttt cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa
    29101 ggtcaaatga gtgcaccggt tcactcttca ttcggctggc atttaatcga actgctggat
    29161 acccgtaatg tcgataaaac cgacgctgcg cagaaggatc gtgcataccg catgctgatg
    29221 aaccgtaagt tctcggaaga agcagcaagc tggatgcagg aacaacgcgc cagcgcctac
    29281 gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt tgtgatcact cccggcgagc
    29341 ccgccgggat tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac
    29401 tggttgtttg tgccgatgcc actctcctta ccgaccgggc agcgatgctc ggtttgccgc
    29461 tcaccctccg cccttattcc cccaactccc ctgcacaacc gcaaactgcg ggcacattaa
    29521 cgctacttcc tgtcgcgcta cgcgaatctg tcactgcggg gcagttagcg attgaaaatg
    29581 ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg
    29641 ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa cgacgcaggt attccgttta
    29701 ctggtcatac cgagtttttc gaagagcgct cgcaggcgaa aaaagtggtg atgatgctgg
    29761 cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc gatatcgcag
    29821 atgctatcac ccctgcactt ttgcacgaag tgattgctat tttgcatcac gatttgcgta
    29881 caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg
    29941 aaggcggtca tatgggtacg gaagagatag acaccattat tccggtgctc gacgagctgc
    30001 gggcgcaggg gatgatactc aacgggccgc tgcctgccga taccctgttt cagccgaaat
    30061 atctcgataa cgccgacgcc gtgctggcga tgtaccacga tcagggtctt cccgtgctaa
    30121 aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt attcgcacat
    30181 cagtggacca cggcaccgcg cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt
    30241 ttattacggc gcttaatctc gccatcaaaa tgattgttaa cacccaatga ataatcgagt
    30301 ccaccagggc cacttagccc gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt
    30361 gatcgacagc attgtttctg ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg
    30421 ccccggtctg gcggcattga ccgagccggt cggcgaacgt ctggaccagc tgacggtcat
    30481 cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat ccattcttag gcccgaaact
    30541 gacgatttat cagcaggatg cgatgacctt taactttggc gaactggccg agaaaatggg
    30601 tcagccgctg cgtgttttcg gcaacctgcc ttataacatc tccacgccgt tgatgttcca
    30661 tctgtttagc tatactgatg ccattgccga catgcacttt atgttgcaaa aagaggtggt
    30721 gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt cgattaagcg tcatggcgca
    30781 atactattgc aacgtgatcc cggtactgga agttccgccg tcagccttta caccaccacc
    30841 caaagtggat tccgccgtcg tgcgcctggt tcctcatgca acgatgcctc acccggttaa
    30901 agatgttcgt gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc gtaaaaccat
    30961 tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc
    31021 ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc cagatggcga actatctggc
    31081 ggagaacgcg cctttgcagg agagttaacg atgatcaatt cgccccgagt gtgtattcag
    31141 gttcaaagcg tctacattga ggcacaatct tcacctgata atgaacgtta cgtttttgct
    31201 tataccgtaa ccatacgcaa tctggggcga gcgccagtgc agttgttggg gcgttactgg
    31261 ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc
    31321 cagccactta tcgcgcctgg cgaagagtac cagtacacca gcggtgcaat cattgaaacc
    31381 ccgctgggca ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt ccctttcagc
    31441 atcgacattc ccgtattccg actcgccgtt cccacactca ttcattaaaa gaatatggcg
    31501 acatacctta ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt gctgcataaa
    31561 gtagaattta cccctgggaa agataccctc tggctgacgg gcgatctggt cgcgcgcggc
    31621 ccgggttcgc tggatgttct gcgctatgtg aaatccttag gcgacagcgt acgtctggtg
    31681 ctgggcaatc acgatctgca tctgctggcg gtatttgccg ggatcagccg caataaaccg
    31741 aaagatcgcc tgacaccgct gctggaagcg ccggatgccg acgagctgct taactggctg
    31801 cgtcgccagc ctttgctgca aatcgacgaa gagaaaaagt tggtgatggc ccacgcaggg
    31861 atcacgccgc agtgggatct acagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg
    31921 ctatcgagtg actcctatcc cttctttctc gacgctatgt acggcgatat gccaaataac
    31981 tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta tcaccaacgc ctttacccgt
    32041 atgcgttttt gcttcccgaa cggtcaactg gatatgtaca gcaaagaatc gccggaagag
    32101 gcccctgccc cactgaaacc gtggtttgcg attcctggcc ctgtcgctga agaatacagc
    32161 atcgcctttg gtcactgggc atcgctggag ggcaaaggta cgccggaagg tatatacgcg
    32221 ctggataccg gctgctgctg gggtggtaca ttaacctgcc tgcgctggga agataaacag
    32281 tattttgtcc agccgtcgaa ccggcataag gatttgggcg aagcggcggc gtcttaaaca
    32341 cagcctgata taggaaggcc ggataagacg cgaccggcgt cgcatccggc actagccgta
    32401 aattctatac aaaattaccg ccgctccaga atctcaaagc aatagctgtg agagttctgc
    32461 gcatcagcat cgtggaattc gctgaatacc gattcccagt catccggctc gtaatccggg
    32521 aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca gatacagttt ctgcgctttt
    32581 ggcaggaact gctcataaac gcgaccgccg ccaatcacca tgatttctgg tacgtcacca
    32641 cacgccgcga tggcttcatc caccgacttc acccacgtta cgcgatcgtc cgtacccggt
    32701 tgactgctga ggataatatt tttgcgtcct ggcaacggac gaccgattga ttcccaggta
    32761 tggcggccca taatcacggg tttatttaag gtgttgcgtt taaaccaggc gagatcggca
    32821 ggcaggttcc acggcatggc gttttccatg ccgataacgc gatctaccgc taacgccgca
    32881 atcagactga tcattgagat ttcccgataa aaaaaattgt cgccactata cgtaaagcgt
    32941 aaaccgtcgt cgactggtgc gaggatgatg ttgaggaaaa ttttatattc tgctggcgag
    33001 tccacgctct ctccctggac tcgccgcatt acaatgaaac aaaaacaaac agttagctgt
    33061 aaagtgtgat ttatgtcact ctttattagg atgagggttt cgtttccggt tcatccgcca
    33121 tgttgccggt atgtttacct tcttcggttc cctgccagcc atgtcgttga attaatgaca
    33181 gatgttcgcg gtcctcggta atgatctcac ttaacatcgc gctggtgcgt ttatagaccg
    33241 ccgcgcgggc tttggtgtcg ttctcaacca ttgccatctc ttccaccatc tgaatattaa
    33301 agcggcggaa cacatcggca cgttctcgcg cttcatacgg ccccagacct aaactttcca
    33361 gtgccagacg cccggttttc agcgcacctt cgaaggtttc acgctccggc ttttcaacgc
    33421 ctgcctgacg caaacgaatg tagtggtcga catcgcgggc gcgggcaata atctgcaaat
    33481 gcgggaagtg ttctttcacc atctctgtca gttgcaggtt ggtttgcgga tcgtcgatgg
    33541 cgttaatcag cacttccgct ttcgccgctc ccgcagattc cagtaaatcc atccgcgtgg
    33601 catcgccata aaacactttc ataccaaatt tacgcaaggt ttcgatatgg tccggatcgt
    33661 gatcgagcac caccattttc accccgctgg agagcagtaa acgtccggtg atctgcccaa
    33721 aacgaccgaa tccggcaatg attacccgcg gctgttcttc gtcgatctca tcggcttcac
    33781 gcgcctcttc agtagaagat tgctcaaggc gattgaggat caccagcaga atcggcgttg
    33841 ccgccatcga cagcgccacc gccagggtca gcgatttcgc ccactccggc tccagcacat
    33901 tcgccatctg cgccgcgcca aataccacaa aggcaaactc actgccctgc cctaacaaca
    33961 ccgcaaacca acgacgctgt ttatttggca cttgcaacgg tcgggcaatc agccacagca
    34021 tggcgatttt gatgatgagg aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa
    34081 gcagcgtgcc aaagtctatc gacatgccaa caccgatgaa aaacagcccc aacagcaaac
    34141 ctttaaaggg ttcgatatcg ctctccagcg catgacggta ttccgagctt gccagcagta
    34201 cgcccgccag aaacgcgccc atcgccatcg acaagccgac ctcttccagc agcaaaccaa
    34261 agccaaacac gaggaataac gccacggcac taaacacttc ccgcaagcca gagcgggcta
    34321 caaaacgcag cgccggacgc gtgacatagc gccccagcaa taccaccagc accagcgcac
    34381 ccgccacttt taacgccgag agagcaaatg cgcccatcgt cgtcgaggca ctgctcgttg
    34441 ccagtagcgg aatcatcgcc accagcggga tcgccgcgat atcctggaac agcagcaccg
    34501 caaaggcact gcgacccatt tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg
    34561 caatcgccgt agaggagagc gccagcgtca tgccgatcaa ttccgcgacc tgccagcgca
    34621 gcccaagtaa catgcagaac agccccagca ggccgccgca aatcaccatc tgcaatgcgc
    34681 caccgccgaa cactgccgca cgcagcttcc acagcctttg tggatcgagt tcgaggccga
    34741 taataaacag catcagcacc accccaatct cggcaaagtg cagaatagat tcggcatcgg
    34801 tcaccagtcg cagcccccac gggccaataa tgcagccggc gatcaggtag ccaagtaccg
    34861 atcccagacc aagacgtacc gcaatgggta caatcagcgc tgccgaaccg agataaatca
    34921 gcgcctgaat cagcgtatgg ctatccatga tgggcctcct gccattccag cagacgttgc
    34981 ttatagtgac gcgcctgccc ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg
    35041 gcaaacggtg gcagccagtt cagcccgcag tagattgccg tcgcctgtag cggctgcgac
    35101 agcacatcaa agcccggatg cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc
    35161 accgcccaca gcaaatgttt gccatgcagc gccttgccgc cgtgaccgta agcccagccg
    35221 tgcgacaaaa ctttatcgat ccaaagttta agaagcggag gaatgctgta ccactgcatc
    35281 ggatgctgcc agacgatcag atcggcgcga gacagcgcct cctgctcggc ggcaatatcg
    35341 atattgaagt caggatagag ttgataaaga gagcgaattt cgacgccttc cagcgtcctt
    35401 gcctgttcaa gcatccgttt attcgcatgg gaatgatgcg gatacggatg cgcataaatt
    35461 ataagaatca taggtagccc gcgtgattac gcctttgttt tcccacaaag tgtagtcata
    35521 tattcacgcg gcttatagtc aataatattg attcagaaaa gagagaaatc tgaacaattt
    35581 accccattct ctgcccgcgg atcgtcatat tgctggtttg cgccaatgac atccctcgat
    35641 gtctccgggg caaacgctac ggaaatcaac aagccaaaca gcgagatacc cgcccccatt
    35701 agcatcgtgt tactgatacc gtaattattg ataaagatcg gcagtgccca ggtcgaaaca
    35761 atggtgccaa tacggcttaa ggacataatc acgcccacgg cagaggcgcg gatatctgtc
    35821 gggaagagtt cattaggata gagccactgc aaattacccg gcccgccaga gaaaaaggca
    35881 tacaccgcaa aggccatcac taccagccag atccccatat ccgggatcag ccccaaaacc
    35941 gccagctcca gcgtcatcat ggcaaagctg ccaatcaaca atggacgccg tccggcagtg
    36001 tttaaccaca gcatcggcgg aatacagccg agcataaaga acaggctaat caccacgttc
    36061 cccagcgccg cgtttttgcc aacacccaat cccaacaaac caacgatttg cgggccaaag
    36121 gtgtaaatgg cgaacattgg gatcacctgg caggtccaga tggcggcaac aaacagaaca
    36181 aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg tttgctgcgg ctgctcttca
    36241 tcgaaagcca ccggttcgcc aaacagtttt atcatcatct cttcgcactc ttttactcgc
    36301 cctttgcgta ataaccagcg gggagattca ggcagttcga atcgaccaat caaaatcaac
    36361 aaacagggga tcgccgcgct acccagcatc cagcgccagc cgccttccac atcataaagc
    36421 cagtagccga ccagatcggc gcaggtcgcg ccgacatacc acatggcggc gataaagctg
    36481 atggaaaatg cccgctgacg ggtactggag aactcggtga tcattgaggt ggcgatggga
    36541 taatcggcac cgatgacaat gccgataagt acccgcatca ccaacagttc gacgggggat
    36601 gaaacaaaca tcgtcgccac cgatatcacg ccgatggcga tgatatcaat gaggaacatt
    36661 ttgcgccgtc cgactttatc ggaaatatag ccaaacagcg atgtgccaac gaacagcccg
    36721 gcgagcgttc ccgcgcccaa caagccaatc cagtcagcgt ccagttttag cgccggagtc
    36781 agttgctcca gcgccacgcc aatcattacc agtacataac catcca
//
LOCUS       DAJTNN010000017        36586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTNN010000017 DAJTNN010000000
VERSION     DAJTNN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.017"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..208
                     /locus_tag="OO150_000787"
     CDS             <1..208
                     /locus_tag="OO150_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5515027.1"
                     /translation="TIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKPEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            355..1872
                     /locus_tag="OO150_000788"
     CDS             355..1872
                     /locus_tag="OO150_000788"
                     /inference="COORDINATES: protein motif:HMM:NF014273.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5515028.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEDDRIKAINEIRMAIHQ
                     VSPFREEPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK
                     NAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQIT
                     AMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1873..3022)
                     /gene="ybdL"
                     /locus_tag="OO150_000789"
                     /pseudo
     CDS             complement(1873..3022)
                     /gene="ybdL"
                     /locus_tag="OO150_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3131..4219
                     /gene="hcxA"
                     /locus_tag="OO150_000790"
     CDS             3131..4219
                     /gene="hcxA"
                     /locus_tag="OO150_000790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5515029.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4229..4426)
                     /locus_tag="OO150_000791"
     CDS             complement(4229..4426)
                     /locus_tag="OO150_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5515030.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4608..6713)
                     /gene="cstA"
                     /locus_tag="OO150_000792"
     CDS             complement(4608..6713)
                     /gene="cstA"
                     /locus_tag="OO150_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5515031.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6894..7307)
                     /gene="entH"
                     /locus_tag="OO150_000793"
     CDS             complement(6894..7307)
                     /gene="entH"
                     /locus_tag="OO150_000793"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5515032.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7310..8056)
                     /gene="entA"
                     /locus_tag="OO150_000794"
     CDS             complement(7310..8056)
                     /gene="entA"
                     /locus_tag="OO150_000794"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5515033.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8056..8913)
                     /gene="entB"
                     /locus_tag="OO150_000795"
     CDS             complement(8056..8913)
                     /gene="entB"
                     /locus_tag="OO150_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5515034.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8927..10537)
                     /gene="entE"
                     /locus_tag="OO150_000796"
     CDS             complement(8927..10537)
                     /gene="entE"
                     /locus_tag="OO150_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5515035.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATASPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQWLASRASA"
     gene            complement(10547..11722)
                     /gene="entC"
                     /locus_tag="OO150_000797"
     CDS             complement(10547..11722)
                     /gene="entC"
                     /locus_tag="OO150_000797"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR5515036.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVLASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12097..13053
                     /gene="fepB"
                     /locus_tag="OO150_000798"
     CDS             12097..13053
                     /gene="fepB"
                     /locus_tag="OO150_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5515037.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLSGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13057..14307)
                     /gene="entS"
                     /locus_tag="OO150_000799"
     CDS             complement(13057..14307)
                     /gene="entS"
                     /locus_tag="OO150_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5515038.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWLVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14418..15422
                     /gene="fepD"
                     /locus_tag="OO150_000800"
     CDS             14418..15422
                     /gene="fepD"
                     /locus_tag="OO150_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5515039.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15419..16411
                     /gene="fepG"
                     /locus_tag="OO150_000801"
     CDS             15419..16411
                     /gene="fepG"
                     /locus_tag="OO150_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5515040.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16408..17223
                     /gene="fepC"
                     /locus_tag="OO150_000802"
     CDS             16408..17223
                     /gene="fepC"
                     /locus_tag="OO150_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5515041.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIKHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17220..18353)
                     /gene="fepE"
                     /locus_tag="OO150_000803"
     CDS             complement(17220..18353)
                     /gene="fepE"
                     /locus_tag="OO150_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5515042.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18568..22449)
                     /gene="entF"
                     /locus_tag="OO150_000804"
     CDS             complement(18568..22449)
                     /gene="entF"
                     /locus_tag="OO150_000804"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5515043.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22446..22664)
                     /gene="ybdZ"
                     /locus_tag="OO150_000805"
     CDS             complement(22446..22664)
                     /gene="ybdZ"
                     /locus_tag="OO150_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5515044.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22667..23869)
                     /gene="fes"
                     /locus_tag="OO150_000806"
     CDS             complement(22667..23869)
                     /gene="fes"
                     /locus_tag="OO150_000806"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5515045.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24112..26352
                     /gene="fepA"
                     /locus_tag="OO150_000807"
     CDS             24112..26352
                     /gene="fepA"
                     /locus_tag="OO150_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5515046.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26527..27147
                     /gene="entD"
                     /locus_tag="OO150_000808"
     CDS             26527..27147
                     /gene="entD"
                     /locus_tag="OO150_000808"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5515047.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27269..27421)
                     /gene="hokE"
                     /locus_tag="OO150_000809"
     CDS             complement(27269..27421)
                     /gene="hokE"
                     /locus_tag="OO150_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5515048.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            27875..28993
                     /locus_tag="OO150_000810"
     CDS             27875..28993
                     /locus_tag="OO150_000810"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5515049.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQVTVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29059..29307
                     /locus_tag="OO150_000811"
     CDS             29059..29307
                     /locus_tag="OO150_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5515050.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPTPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29372..29740
                     /locus_tag="OO150_000812"
     CDS             29372..29740
                     /locus_tag="OO150_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5515051.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            29834..30487
                     /gene="nfsB"
                     /locus_tag="OO150_000813"
     CDS             29834..30487
                     /gene="nfsB"
                     /locus_tag="OO150_000813"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5515052.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            30595..31842
                     /gene="ybdG"
                     /locus_tag="OO150_000814"
     CDS             30595..31842
                     /gene="ybdG"
                     /locus_tag="OO150_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5515053.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(31910..33286)
                     /gene="pheP"
                     /locus_tag="OO150_000815"
     CDS             complement(31910..33286)
                     /gene="pheP"
                     /locus_tag="OO150_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5515054.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYICSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33388..36515)
                     /gene="cusA"
                     /locus_tag="OO150_000816"
                     /pseudo
     CDS             complement(33388..36515)
                     /gene="cusA"
                     /locus_tag="OO150_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
BASE COUNT         8789 a        10214 c         9312 g         8271 t
ORIGIN      
        1 gacaataatt aatagcattg cagttacaga tttactggga atagtcccct atgaacttta
       61 taactcccac cgtgactttc ttaatttaaa agagataaaa ccagaacatc cgttaccttc
      121 aataaagctt tacatttcct ataataaatc ttctttaaat aatttggtgt tctcccgatt
      181 tattgaccgt ctaaatgaga gtttttgata tataactaca gacagctact ataactttca
      241 tctatttatt cacagcgcga atgaaaaata aaacactctg ctttacagtg tttactttat
      301 aattaacagt cttatctcac tgttcaccct gtaacgttca gagagcgtct tcagatgtct
      361 atttataaaa ttcctcttcc gctcaatatt ctggaagctg cacgagagcg tattacctgg
      421 acactcaaca ccctaccccg cgtttgtgtc tcattttctg gcggcaaaga ctctggttta
      481 atgctgcatc tgacagccga acttgcccga caaatgggca aaaaaatctg cgttttgttt
      541 atcgactggg aggcgcaatt ctcttgcact attaactatg ttcagtccct gcgcgagttg
      601 tacaccgatg tcatcgaaga gttttactgg gttgcgctcc cgcttacgac gcaaaattcc
      661 ctttcacaat accaacccga atggcagtgc tgggaacctg atgtcgaatg ggtgcgtcag
      721 cccccgcaag atgcgataac cgatcctgac tttttctgct tttaccagcc aggcatgacc
      781 ttcgaacaat ttgtacgtga gtttgccgaa tggttttcac aaaaacgtcc ggcggcgatg
      841 atgatcggca tccgtgcgga tgagtcctac aaccgttttg tcgccatcgc cagtttaaat
      901 aaacaacgtt ttgccgacga taaaccatgg actacggccg caccaggcgg tcatagctgg
      961 tacatttacc ccatttacga ctggaaagtg gctgatatct ggacctggta tgcaaatcat
     1021 caaagtctct gcaacccact gtataaccta atgtatcagg caggcgttcc tctgcgtcat
     1081 atgcgaattt gcgaaccttt tggcccggag caacgacaag gattatggct ctatcacgtt
     1141 atcgaaccgg atcgctgggc tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc
     1201 atttacgccg gacatgacaa tcatttctat gggcatcgga aaatcctcaa gccagaacat
     1261 ttagactggc aagaatatgc attattgctg ctcaatagca tgccggaaga tgatcgcatc
     1321 aaagcgatta atgaaatccg catggcgatc catcaggtta gcccctttcg tgaagagcca
     1381 gtggattgcg ttctgtgggt taaaaacagc caacttatgc ctaatgatta caacccaaac
     1441 aacgtggcac cacccgaaaa aaaactgctg caaaaatcca ttgaaataga tggttttacc
     1501 caacccattg tggtcacaca tacggataaa aatgccatgg aaatcgtgga tggttttcac
     1561 cgccatgaaa ttggtaaagg ctcatcatca ttaaaattac gcttaaaagg ttatttgccg
     1621 gtgacctgtc tggaaggtac gcgtaatcaa cgcatcgccg cgactattcg tcataatcgc
     1681 gctcgtggac gccatcaaat caccgccatg tcggaaatcg tccgtgaact tagccagttg
     1741 ggatgggacg ataataaaat tggcaaagag ctgggaatgg acagcgacga agtgctgcgc
     1801 ctgaagcaaa ttaacggcct gcaagagttg tttgccgacc gtcaatattc ccgcgcctgg
     1861 acggttaaat agctaaagct ggcgcaggcg ttcagctgct gccagcaacg tcgattcctt
     1921 cttggcaaaa cagagacgaa tcagtttatg tgggaaggga tcggcgcaaa acaccgacag
     1981 cggaatcgcc gccacgccgt gctcccgcgt cagccactgg caaaactcaa catcatccag
     2041 ggtagaaacc tcgctgtaat ccaccagcaa aaagtatgta ccttcacacg gtaaaatctc
     2101 cagccggctt tcatttaaag cattcaccag aatatcgcgc ttctggcgat aaaagtccgg
     2161 taacgcaaga taatgctcag gttctgcacg tagcatatca gcaagcgcca gctgtgccgg
     2221 ggtattcacc gaaaaggtca gatactgatg taccttgcga atttcggcgc tgatgggcgc
     2281 tggcgcaaca caataaccca ctttccagcc ggtcatatga tagatcttgc caaatgaaga
     2341 aaccgccact gcccgctcac gcagctgcgg atgcgccagc acactggcat ggccctgttg
     2401 tgaaaagttg atgtgctcgt agacttcatc gctaatgaca aaaatctcgt gcccggcgat
     2461 cgcctgccac aaagcggcga aatcagcctg ctgccagaca gttgcactgg ggttatgcgg
     2521 agtgttgagg atcaccagtc tggtgcgctc gcttaataat gcggcaaatt cctgccagtc
     2581 aaggtggttg cagtgccata cgcttcacta ttcccccaga aagcgcgatg gcgggggcgt
     2641 aactgtcata gctgggatca aaacaaatca cttcatcgcc attgcgcacc agtgcggtaa
     2701 tcgccgcgta taacgcttcc gtcgcccctg ccgttacggt gatatcgcta tcggcatctg
     2761 gttgatagcc atacaaacgt tccgttttct gagcaatcgc ctcgcgcaag gcctgcacgc
     2821 cggtcatggg cgcgtattgg tttgccccct gggcaacgtg gtacgccagc cgctcctgta
     2881 aatagcgcgg accatcaaaa tcaggaaagc cttgcgacag gttaatcgcc tggtgttgct
     2941 gcgccagcgc gctcatctgg gtgaaaatag tggtgccaag ttgtggaagt ttgctttgtg
     3001 gaatcagagg gttatttgtc atttttattg tacctgacga agttgcgtgt tgaaggcact
     3061 ataacacgac gctagtattt ggcaatcaag acgtttagat gtctaaataa aacaataagg
     3121 acaacacaac atgcctcaca atcctatccg cgtggtcgtc ggcccggcta actacttttc
     3181 acatccaaga agtttcaatc acctgaacga ttttttcact gatgaacaac tttctcgcgc
     3241 ggtgtggatc tacggcaaac gcgccattgc tgcggcgcaa accaaacttc cgccagcgtt
     3301 tggacttcca ggggcaaagc atattttgtt tcgtggtcat tgcagcgaaa gcgatgtaca
     3361 gcaactggcg gctgagtccg gtgacgatcg cagcgtggtg attggcgtcg gcggcggcgc
     3421 actgctcgac accgcgaaag ccctcgcccg ccgtctcggt ctgccatttg ttgccgttcc
     3481 gacgatcgcc gctacctgcg ccgcctggac accgctctcc gtctggtata acgatgccgg
     3541 acaggcgctg cattatgaga ttttcgacga cgccaatttt atggtgctgg tggaaccgga
     3601 gattatcctc aatgcaccgc aacaatatct gctggcgggg atcggtgaca cgctggcgaa
     3661 atggtatgaa gcggtggtgc tggctccgca accagaaacg ttgccgctaa ccgtgcggct
     3721 ggggatcaat aatgcgcagg ccattcgcga cgtcttgtta aacagtagcg aacaggcact
     3781 tgccgatcag caaaatcagc agttaacgca atcattttgc gatgtggtgg atgctattat
     3841 tgctggtggt gggatggtcg gtggtctggg cgatcgtttt acgcgtgtgg cggcggctca
     3901 tgccgtgcat aacggtctga ctgtcctgcc gcaaaccgag aagtttcttc acggcaccaa
     3961 agtcgcttac ggaattctgg tgcaaagcgc cttgctgggt caggatgatg tgctggcgca
     4021 attaaccggg gcgtatcagc gttttcatct gccgactacg ctggcggagc tggaagtgga
     4081 tatcaataat caggcggaga tcgacaaagt gattgcccac accttgcgcc cggtggagtc
     4141 cattcattac ctgccagtca cgctgacacc agatacgttg cgtgcagcgt tcgaaaaagt
     4201 ggaatcgttt aaagcctgac gtgcaaattt agcagcagcg cgcgccgcct tttccaccgt
     4261 agcgcgcgtc ctgccgctca cggaaaaact cctcgtaggt catcggcgtt tgatcgggat
     4321 ggttaacccg catatgttcg acatagttgt cgtaatcagg cataccaatc atcagcttcg
     4381 ccgcctgacc taaatatttt ccggcttttg ccagtgaatc aaacatcttg gcctccgcta
     4441 accatgttgc tgaaagagtg ccggatgcgg cgtgaacgct ttatccggcc tacaaatgct
     4501 atcaacgcac tcggtcagtc ccctcgcccc atcggggaga gggttagggt gagggggaac
     4561 cctcggcact atgccaacat tcgccaacat cccccctcac tctgacttta gtgcgcgcct
     4621 ttcgcctgcg ctacgatctc ctcgacattt tccggcatcg gctcatacgg cgtttctttc
     4681 gccgttggct tcggatcttt caatgccgca agtgccgtct taatcgagaa cagtgccaga
     4741 accaccacga ccaccataaa gaagatggtt aacccggcat ccagacggtt gttgaacacc
     4801 agttgcgcca gctgtgactc agtatactgc gacggaatat tgccgctgtc gatcattgcc
     4861 tggaacttgt tagcaatggc caggaagccg actttcgcat ccgggctaaa cgctttctgc
     4921 cagccagcgg tcagggtaca aatcagcagc caggccgttg gtaccagcgc cacccaggcg
     4981 taacgttgac gtttcatctt gaacaacacc acggcacaga gcatcagcgc catccctgcc
     5041 agcatctggt tggcaatacc aaacagcggc cacagagtgt taataccgcc cagcggatcg
     5101 accacgccct gatggaggaa gtagccccac gccagtacgc acagcgccgt tgccagcagg
     5161 ttagcaggca gtgaatcggt ccgcttcagg ccaggagaca ccacgcccag cagatcctgc
     5221 aacataaagc gcgcagcacg cgtacccgca tctaccgccg tcagaataaa cagagcttca
     5281 aatagaatgg cgaagtgata ccagaacgcc acatccatca taccacccag cgcaccgtgc
     5341 aggatgtaag ccatccccac cgccaacgtc ggcgcaccgc ccgcacggga aatgatcgac
     5401 tgttcaccca cttcgctggc aatctggttg agcgtatctg gtgtaatgct aaagccccag
     5461 ctactcacca cctgcgcggc agaagcgacc acatccgccg tccctgctgg agccagcacc
     5521 gccattgggc tgttcatggc aaagtaaacg cccggatcga tgatacaggc ggaaaccagc
     5581 gccataatcg ccacgaagga ttccattaac attccaccgt agccgataaa acaagcctgc
     5641 ccttcgttcg ccagcatctt cggcgtggtg ccggaagaga tcagcgcatg gaagccagac
     5701 accgcgccac aggcgatggt gataaacagg aacgggaaca ggttaccggt ccataccggg
     5761 ccagtgccat cgacaaattt ggtcagcgca ggcatggtca gcgtcgggcg cataatcaaa
     5821 atgcctaccg ccagaccgac gatcgtcccg attttcagga aggtagagag gtagtcacgc
     5881 ggggccagca gcaaccacac cggcagcacc gccgccacaa aaccgtaacc caccagcatc
     5941 caggtcagct gcacgccggt aaagtcaaag tacggtgccc aggtcggact ttctgccacc
     6001 cagccgccag agataatggc gaaaatcagg aataccagac cgatgaccga cacttcacca
     6061 atacgccccg gacgcagata gcgcaggtag atccccataa acagcgccag cggaatggtg
     6121 aacgcaacgg tatacgttcc ccacgggcta tgagtcaggg ctttcaccac gatcatcgcc
     6181 agtactgcaa ggataatgac catgatcata aagcaggcca ccagcgctat caccccggcg
     6241 gttggcccca tctcttcttt gaccagctca cccagcgatc gaccgtcacg gcgcgtagaa
     6301 acaaacagca ccatgaaatc ctgcaccgca ccggcgagaa ccaccccggc gagtagccag
     6361 atcatccccg gcaggtagcc catttgcgct gccagcaccg gcccaaccaa cggacctgct
     6421 ccggcgatgg ccgcaaaatg gtgaccgaac agcactttct tgtccgtcgg cacatagtcc
     6481 agcccgtcgt tatggcgcac cgctggcgtc atacgcttcg ggtcaaccgc cagcacattt
     6541 ttggcgatat acaacccata aaaacggtaa gcgatcagat agatacagac cgacgccacc
     6601 acaatccaca gcgcgttgat ctgttcccca cgatttaaag caatgtatcc cagagcaaat
     6661 gctcccatta cagagagcac tgtccagacg aggtatttcc ctgatttgtt catagttgtt
     6721 atccgtgtgc gtgtccatag agatgttaca ttttgtttct ataacatctt tgtacgattt
     6781 aacaacacgg taactatgta aatcggagtt gaaaccaata tttaacttaa caatgttaac
     6841 tcgatcactc cgttaaccga gttttcctgc aatctcattt aattctgtcg ggctcatccc
     6901 aaaactgccg tacccagccg acaagtgcag caacgccgcc cctgttcatc gaaaacgacg
     6961 atttcccagc tctgattttg ccgcccaaga tgcagcggct ggcagacgcc gcgtaccttg
     7021 ccttcagaca ccgggcgatg atgggtggcg ttaagttccg tgcccaccac acactgcccg
     7081 tcacgggtca tcataaatcc ggccatcgat cccagcgttt cagccagcgc cgccgacgcg
     7141 ccgccatgca gcaggccaaa cggctgatga gtacgggtat caaccggcat ttcggcttcc
     7201 agcacatcat ctcccagacg ggtatacaca attcccagat gcgccaccat tgtgttatcg
     7261 ctggtggcgt tcagttcgtc gagcgttaaa tggcgtttcc agatcatgct tatgccccca
     7321 gcgttgagcc gccatcgacc acaatatcct gtagggtgat atggctggcg aggtcagagg
     7381 cgaggaacaa aatcgtgttg gcgatctctt gtggacgggc gattttcccc agcggaatgc
     7441 cgagtttaaa ctgctcgcca aagccgcgaa tacgctgttc ttcggcgtca tcgctcaccc
     7501 acagcgtgcg ttgcatatcg gtgtcggtgg agccaggcga aaccacatta cagcgcacgc
     7561 cgctacccgc cagttccagc ccgacgctca acgccaggct tttcagcgcc gctttcgatg
     7621 cgccataagc actcatacca atacgcggcg tgtgtgcggc gtcggacgcc acagtgacaa
     7681 tcgccccgcc ccgctgacgg cgaaactggt tcatggtttg ctggaacagg ttaaacgcac
     7741 cgccgacgtt aaccgcaaaa gtctgctgcc agtcctcttt actgagctga tcggtcgcgc
     7801 ccatgcgcaa aattcccgcc gcattgatca gcacgtccag tcgctccgtt tcagctaaca
     7861 gtcgctgaca tacctgcgcc acctgcccgg cgtcggcaac atccattact tcggtcgcaa
     7921 agggatattg ctcctgagtg aacgcttgat caaaacctgt aactttcgct cccgcctcaa
     7981 caaacgccag cgccgtggcg tagccgatac ctttacctgc gccggttacc cagacatttt
     8041 taccgctgaa atccattatt taacctcgcg ggagagtagc ttccaccagg cgtcgatggt
     8101 cgggtttttc gccagcatga caaagtcgat atcaccatgc actttgcgcc agcgcgccgc
     8161 cagcgccatc atgcgcaccg aatccagacc gtagtcgatc aggttgtcgt catcgaacgg
     8221 ttcatcggac tcgtccagca acggcaggat aacctcacgc agcgccgctt tgctggcggg
     8281 gacaggtgct ggcagtaatt cttcagtcat caccacccgg ccagaccgcc cggccacata
     8341 tttcagcgac atcaaatgct cgtcacggct gaaatcggcc agcgcatccg ccaccataaa
     8401 cggtttaata tcgcgcataa atgcgtcggt tgcggtggtc atacagccaa tgtgggcata
     8461 taccccggta ataatcagct ggttacgtcc actctctttg agcatttgct ccagcggaga
     8521 acgatgaaac gcgctgtagc gccacttcac cagcaccgtg tcgtcggcat ctggcgtcag
     8581 gcgatccacc accttttgct gttccggcga acgggtcagg cccggccccc acatatcatt
     8641 caacagcgcc cgatcttcat cgctctgctc tttcggctgg gcggtgtaat aaactgggat
     8701 attgtgctgt ttgcagtagt cgcgcagcgc agcaatattc gcgatcacct gctccatcat
     8761 cgggcagttc tcgccccaga agctgacaaa atagtcctgc atatcatgga ttaacaacgc
     8821 ggcacgttgc agttcaaagg cccagtcaac tttattctgc ggaatatcgt gagactccgg
     8881 cagtgcgtaa gcctgtaatt ttggaatagc catcgtgttc tctccttcag gctgatgcgc
     8941 gtgacgccag ccactgactt aattgttttt tatcgacttt cccgaccgcc gtcagcggaa
     9001 gtgaatctac acactccacg cgatccggta atttaaattc ggcaataccc tgttcacgca
     9061 ggaaacgacg cacctgcacc gcgcgcagcg gctcttttac caccagataa gcacagcttt
     9121 tttcgcccat cagctcatct tccatgctca ccagtgcggc atagatcacc gccggatggc
     9181 gcagcagcag gttttcgatc tcttcggcag cgatcttctc gccgccacgg ttgatctgat
     9241 ctttctcgcg cccctgcacg gtgatgtaac cctctggatc aatagagatc agatcgccgg
     9301 aacagtaaaa accgttggca tcaaaggcgc tggcattgtg ctgcggactt ttgtaataac
     9361 cgcggaaggt gtacggcccg cgcgtcatca ggcgtccgac ttccccttgc ggcagtggat
     9421 ttccttcggc atcggcaacc cacacttcat cgtccggaca cattgggtaa ccctgggtat
     9481 ggataatttt ctccgcgcta tcatcaagac gggtgtagtt caccagccct tccgccatgc
     9541 caaacacctg ctgcaactgg cagccaatct cagcgggaat acgcgccgca agggtggcag
     9601 aaagacgtgc gccgccgacc tgtaacagtt tcagcgaggc aagctgcgcc cggctttcgc
     9661 cttcagccag cgcctgcaac cacaggctga ctgccggcgg caccagcgcg gtgacgttca
     9721 cctgatgttt ttcaatcaat gggaagcaaa gcgtagcgct gggatcggcc gccagaacaa
     9781 ccgttcctcc ggcaagaaag acgcccagcg atcctggcga actcatggcg tagttatgag
     9841 ccgccgggat cgcgcacagg tagcgcgtct gttgtgtgaa ctgacaaatc tcgacgctac
     9901 gacgcacgct gtagtagtag tcgttatgag tgcgcgggat cagcttcggt gtaccggtgg
     9961 tgccgccgga aagctggaaa taggccactt catcagcagg tgatgccgtg gcagtaaaac
    10021 cgtctgccgg atggttaatc gcatcctgca agttatgctc accgctgtcg ttgtgcagtt
    10081 gcaccacgcg aatggaggaa tgttctgtga cgaaagtatt gaggaaatca tccccgctaa
    10141 acagcgcatg ttggcgatcg gcaatcagca atgcgggttc aatctggctg gcataggcgt
    10201 tcagttcact acgctgatgg ctgaacaacg ccagcaccgg cgcaacgccc agtttcagca
    10261 gcgcgaaaaa ggtaatatac aattcagcga cgttacccag ttgtaccagc gcggtttcac
    10321 caggtttaat gccctgacgg cgtaaagtac acgcgaggtt atccgccgcc tgattcagct
    10381 cccggtaact caactgtagc tcgccgtcga taaccgcgat gctgtcactc gcagcgtggc
    10441 gagtcagaat gtcggtcagc ggcaaatcct gccagtagcc tttttcccga tagcgacggg
    10501 caaactcttc cggccagcgg gtgaatggaa tgctcatcct cgctccttaa tgcaatccaa
    10561 aaacattcaa catggtagaa agtttgacgc ctgtttcgcg ccactcaccc aacggtgacg
    10621 acgcaagcac aatccccgct ccggcaaaca gacgcacctg attttcccgc agcttcgcgc
    10681 agcggatggt caccacccat tcgccgttac cttcgctgtc acaccaaccc acaatgccgc
    10741 caaacagttc gcggtcgaac ggctccagtt cagcaataac ctgagtcgcg gcctgatgcg
    10801 ggaaaccgct cagcgcgggg gtcggatgca gcagacaggc cagagtcagt gcgttttctt
    10861 gcgaattcgc tttaccttca aagggagttg cgagatgcca cagcgtcggc gtggtaatca
    10921 attgtggaga ggagggaacg tgtaactcac tactgcgttc gcgcagtacc tctttcatcg
    10981 cctgagtcac cagttcatgt tcatggcgat ctttttctga cgccagcaga cgattacccg
    11041 cttcgcgatc gagcacttca tccggctgac gacgcgcgga accggctaac ggaatggagc
    11101 taaaacgctc gccgtcttta cgtagcagca gttccgggct ggcccccagc aggacgccac
    11161 catcagccag cgggacatgg aagttgtaac taaccgggtt ttgcgcaatc aaccgttcca
    11221 gcaatacgcc actatcaatg gcggcgtcag tggtgatatc aatcaaccgt gacaacacca
    11281 ctttgtcgac ctgcggcgtg gcggtaagtg cggcagcgcg ggcaaccatc tgttcaaacg
    11341 tggtttgttc cggaattgcc tggcgttcca ccacattcag cgactggctg cgggtgaaac
    11401 ggcgggcgga agcttgtttt tcctgacggg agaacgactg ccaggattca ggaatataca
    11461 gcgacgaagg ctgacgtgga tcgaagggaa tcgccccgac catcaccgga tttttgatgc
    11521 cctgcacttt ggcatcggca aacagcgcgg cgagtttttg ctggaaggga ctgtcgggcg
    11581 aatccccgtt cacagccggt tcatcgaagc gggcgaaaca tcctgacgtc gtaaaactgc
    11641 ggtacggcga cataaagaaa aagcgattgg gcgcaagtgt tgccatggtc tgctgtactt
    11701 cctcagccag tgacgtatcc atatcatcct ccacaaaatg ataaaggctt taataatgat
    11761 tatcatttat attttcggtc gctaacctaa aagcaaacgc gcactatgtc aactcttgag
    11821 gtaacgcaat ctgcaactcg gggttgcatc tggttgtggt tacaatgaaa atgagaagca
    11881 ttattgatgg attcgcataa gcgcaatgtg atgtcctgcg ccgttcttcc ccctctccct
    11941 tccagggaga gggctggggt gagggtcaat attcgcacca gtgctggctg ctcccatcac
    12001 cctaaccctc tccccaaagg ggcgagggta cggatagtgc gctttgtcga atttgtcatt
    12061 acgcccttaa ccttattaat aacaggaagc tgatttgtga gactcgcccc gctctaccgc
    12121 aacgcccttc tattaacagg acttttgctt tccggaatag ccgcagttca ggccgccgac
    12181 tggccgcgtc agattactga cagccggggc actcataccc tggaaagcca gccgcagcgt
    12241 attgtttcca ccagcgtcac cctgaccggc tcactgctgg cgattgatgc tccggtgatt
    12301 gccagcggcg cgaccacgcc aaataaccgc gtcgcggatg accagggctt tttacgccag
    12361 tggagcaagg tggcgaaaga acgcaaacta caacggctct atatcggcga accgagcgcc
    12421 gaagccgttg ccgcgcaaat gccggatctg attttaatta gcgcaaccgg cggggattcg
    12481 gcgctggcgc tgtatgatca gctttccacc atcgccccga cattaatcat caattacgac
    12541 gacaaaagct ggcagtcgct gttaacgcag cttggcgaaa ttaccgggca tgagaaacaa
    12601 gcggcagagc ggattgcaca gtttgataag caactggcag cggcgaaaga gcaaatcaaa
    12661 ttaccgccgc agccggtcac tgctattgtc tataccgccg ctgcacacag tgccaatctc
    12721 tggacgccgg aatcagcaca agggcagatg ctggaacaac tcggctttac gctggcgaaa
    12781 ttgcccacag ggttaaacgc cagccaaagc cagggcaaac gccatgacat cattcagctt
    12841 agtggagaaa atctggctgc agggttaaat ggcgagtcac tattcctgtt cgccggtgat
    12901 cagaaagacg ccgatgcgat ttatgccaat ccactgctcg cgcacctgcc cgcagtacaa
    12961 aacaagcagg tttatgcgct gggaaccgag acgttccgcc tggattacta cagcgccatg
    13021 caagtgctgg aacgacttaa ggcgctgttt taagcattaa ctgtcggacg ctgtcacctg
    13081 cggcggcgtc tggcgaaaac gtcgcaactc caccagcacc agcaataaca acacgccgat
    13141 aatcaacaaa ccaaaaccgc tcgcgcttgc ggaagcaacc ggtgtcatca tcgctcccag
    13201 accgcctaac agcgccgcgc ctatggcatc gcccgtcacg ttttgtgccg tccacaaacc
    13261 gttgattcgc cctaacattg cttccggcgt ttgcgtttgc agcattgtat attgcagcaa
    13321 cgaactgacc gcactcagcc agccgaacag cgccagacaa accacgccta aaatccacat
    13381 cggcatcagg ccaaataaac caatggcgag gaacgatccc aacgtggaga gcaacatcaa
    13441 caatcctggt cgcgcgctat gcgccagttt cccactggtt aatgcgccaa tagccgcacc
    13501 gagcgggatc gccgcataga gaaaaccaat ctctgccgcc gacatctgcc agttgtcagc
    13561 cagcgccgga tacagtaccc gcaccgcgct cgccatcgtc aataaaccac ccagcaacgc
    13621 aatcccgccc accagcgggc tggcgagcag aaaacgaaat cctgccagta atgatttcaa
    13681 cggatgctcg cgcggctgcg gtggcggtgg caacgccgga aggcttaaca acggtagcaa
    13741 ggtaataaac gtgcccgccg ccgccagccc gtagttccag gctacgccac cggtcgccag
    13801 caataaaccg ccaatcatgg gcgaaatcac cgaccccaga cgcacggtca acatggtgat
    13861 cgccccggcc tgcattaagt tttcacgccc taccagtgct ggtgtcgccg ccaatagcgc
    13921 cgtaacgcca agcgatgcga aaaaaccatc ccataaacca agtaaataga ttgccagcaa
    13981 tgacggctcc ggcagcagtg cattaaggca cagtccaatg aagccaatgc cacaggtgcc
    14041 gcgcgccagc aaaatcactt ttttgcgctc atagcgatcc gccagcacac cgccaaccat
    14101 caggccaaca aacatcgcgc cgccggtcag cgtcaccgaa agccccacca gccaggtaga
    14161 atgcgtcatc atctggatct gcaccggcac cgcgacgccg agcaaaccca gagacacaat
    14221 tgagataaaa cgagcgagga atactgcgcg aaacgccggg tgcgttttca acaggctgag
    14281 gttaagcagc caggattgtt tattcattac aatgccttgc catcaaatat tttaaatacc
    14341 tattcttagg ctgcacatgc taacatatcc aaataagatc gataacgata attaatttca
    14401 ttatcatgga agttcgtatg tctggttctg ttgccgtgac acgcgccatt gccgtgcccg
    14461 gattgctgtt attactgatt atcgcgacgg cattaagcct gctcattggg gcaaaatcac
    14521 tccccgcttc cgtagtgctg gaggccttct ccggcacctg ccagagcgcc gactgcacca
    14581 tcgtgctcga cgcgcgactg ccgcgcaccc ttgccggttt actggcaggc ggcgcgcttg
    14641 gccttgccgg ggcattgatg caaaccctca cccgaaatcc acttgccgac cccggcttgc
    14701 ttggcgtgaa cgccggagcc agctttgcca ttgtgctggg tgcggcgctg tttggttact
    14761 catccgcgca ggaacaactg gcgatggcct tcgccggggc gctggtggcc tcattgattg
    14821 ttgcctttac cggcagtcag ggcggcgggc agttaagtcc ggtgcgttta accctggcgg
    14881 gcgtggcgct ggcggcagtg ctggaagggc tgaccagcgg catcgccctg cttaatcctg
    14941 acgtctacga tcaactgcgt ttctggcaag ccggttcgct ggatattcgc aatctacata
    15001 ccttaaaagt ggtgctgatc ccggtgctaa tcgccggagc aaccgcgcta ttactgagcc
    15061 gcgcgctgaa cagtttaagc ctcggcagcg acaccgcgac ggcgctgggc agtcgcgtgg
    15121 cgcgcacaca gttaattggt ctgctggcga ttaccgtgct ttgtggtagt gcgacagcgg
    15181 tagttggccc gattgccttt attggcctga tgatgccgca tatggcgcgt tggctggtgg
    15241 gtgccgatca tcgctggtcg ctgcccgtaa cgctgcttgc tacccctgcc ctgctgctgt
    15301 ttgccgatat catcgggcgg gtgattgtgc ccggtgaact gcgcgtttct gtggtcagtg
    15361 cctttattgg tgcaccggtg ctgatcttcc tcgtgcgacg taaaacgcga ggtggtgcat
    15421 gatttacgtc tctcgccgat tactcatcac ctgtttgctg cttatttccg cctgtgtggt
    15481 tgcaggtatc tggggattac gcagcggtgc cgtcacgctg gaaacctcgc aggtattcgc
    15541 cgcgctgatg ggcgatgcgc cgcgcagtat gacgatggtg gtcaccgaat ggcgtttacc
    15601 acgcgtgctg atggcgctgt tgattggcgc ggcactgggc gtcagcggtg cgatttttca
    15661 gtcgctgatg cgtaacccgc tcggcagccc tgacgtaatg ggctttaaca ccggggcgtg
    15721 gagcggcgtg ctggtggcga tggtgctgtt tggtcaggac ctgacggcta tcgcgctggc
    15781 agcaatggtg ggcggcattg tcacttctct gttggtctgg ctgctcgcct ggcgtaacgg
    15841 tatcgacacc tttcggttga ttattatcgg catcggcgtt cgcgccatgc tggtggcctt
    15901 taatacttgg ctgttgctga aagcgtcttt agaaacggcg ctaacagcgg gtttgtggaa
    15961 tgccggatcg ctcaatggcc tgacgtgggc aaaaacttcg ccttccgcac ccatcattat
    16021 attgatgctc attgccgccg ccttgctggt gcggcgaatg cgcttgctgg agatgggcga
    16081 tgataccgcc tgcgcgctgg gcgtcagcgt cgagcgttcg cgtctgttaa tgatgctggt
    16141 tgcagtggtg cttaccgctg cggcaacggc gcttgccggg ccgatttcct ttattgcttt
    16201 agtcgcgccg cacattgccc gacgcattag cggcaccgct cgctgggggc tgacccaggc
    16261 ggcgctgtgc ggagcgctgt tactgctggc ggccgatctc tgcgcccaac aactgtttat
    16321 gccgtatcaa cttccggttg gcgtcgttac cgtcagcctc ggcggtattt accttatcgt
    16381 cttgttaatt caggagtctc gcaaaaaatg accgaatcag tagcccgttt gcgcggcgaa
    16441 cagttaaccc tgggatatgg caaatatacc gttgcggaaa atctgactgt agaaattcct
    16501 gatggtcact tcacggcaat tatcggacca aatggctgtg gtaaatccac gttactgcgt
    16561 accttaagcc gcctgatgac gcctgctcac gggcatgtct ggctggatgg cgagcacatt
    16621 aaacattacg ccagtaaaga agttgcacgc cggattggtc tgttggcgca aaatgctacc
    16681 acgccgggcg atatcaccgt gcaggagctg gtggcgcgtg gacgttatcc gcatcaaccg
    16741 ctgtttaccc actggcgcaa agaggatgaa gaagcggtaa cgaaagcgat gcaggccacg
    16801 ggaataactc atctggcaga tcaaagcgtg gatacccttt ctggcggaca acgccagcga
    16861 gcgtggatcg cgatggtgct ggcccaggaa acggcaatta tgctgctcga cgaacccacg
    16921 acctggctgg atatcagtca tcagattgat ctactggaac tgttaagcga actgaaccgc
    16981 gagaaaggct ataccctggc ggcggtgctg cacgatctta atcaggcctg tcgttacgcc
    17041 agccatttga ttgcattgcg ggaagggaaa attgttgctc agggagcgcc gaaggagatt
    17101 gtcactgctg aactgattga gcgcatttat ggtctgcgat gcatgatcat tgacgatccg
    17161 gtagccggaa cgccgcttgt ggtgccgctc gaacgaacgg caccttcaac cgcaaatagt
    17221 taaactaagt ggtctgccat catggcatcc tgttttctgg atgccatcgc atagcgcaat
    17281 aacacgctac cacaagccac catcccgccg atcagcgcgg aaaggatcac aataatcgcc
    17341 ttacccggac cgtctttttt cactggcaat gacgggctta actgatattt aaacggcgta
    17401 aaattcacat cgttgacatt tgcttttgtt aattgctcga caagatactg ccgattacgt
    17461 aattcaccgt tcagttccgc aacgtcagta accgcttttt caatttccag tttgcgttca
    17521 ataccgtctg cgccgagaga aatagagaaa tcggggtcat ctttaacggc ctgaccatta
    17581 ctgtatacgg gctttttaat tcctgccgcg ttggcaatgt cgagtgaata attgaggcgc
    17641 tgaatgtttg catcaagttg atttttcgtt ttaatgcgat cctgagccag tttttctttt
    17701 tcaaactggg ttttgatctc cagtttatta cggacgtttt ctatcgactc tttcaccacc
    17761 aacgcagaga tataatcgat atacccgctc aaaacggtct gcgcctcttt actggttggc
    17821 gcggtaaaac ttagcgtcca ggaggtatac agtgacggtt catctttttt cttactggca
    17881 ttgtcatcaa ccgctttcat tttttcgctc aatgcaacaa tagcgcgatg caaatccagt
    17941 tcatcgattt tcgcctcttt taattggtcc atcacataag gtgatgaacg caggtactct
    18001 tccagcaagc taacagactg aaacttcttg ataaacaggt taaatgcacc gccacggtcg
    18061 atattaacat cgagatcaag aacgcggagt ttggtaaagg ttttctccag gtcctgccac
    18121 tgaatggctt cggcgggagt aataacggct gagctggtcc atttttgcgg caggatgaaa
    18181 gagatcagta aacctgcgca ggcaaatgca aaaacgaccg ccatgaccgt ttttttggcc
    18241 cgccataaaa cttcgattag attaagtaaa tcaatttcat tactactggg cgacgccaga
    18301 ggatactcgg aaaaatgagc gtcacttccc tgtttaatat tcagtgatga catgggtata
    18361 acctgaaaat gagtccaata gggggaaatc tccacggcgc tcactttacc agttggtgag
    18421 aattatccga agctgaagtt tgtaaattcc ttccactgaa tgcaacattc actctattta
    18481 agagaaatat tcatcaaacc tataaatcaa acaaataaaa aacccataat tattttctgt
    18541 aattatgggt ttatagataa tattaattta cctgtttagc gttgcgcgaa taatcggccc
    18601 aatttttaca aacgcccctg gagagataat atccacatgc gcacaatcct gacgatagat
    18661 atccagctcg gcaatccagg gcgaccaagc gcgttcgggg ctcatacctt cctgaagcgt
    18721 gcgttcagca acaaacagcg tcgctttacc atcaaacggt acgctatgag cagtcgtcag
    18781 cagacgcaca gcatcagcgt agttgccttc aatggtggta aacaactccg ttgaagtact
    18841 tccctgctgt gccgccagga aggcctcgcg ttcgcggtta atctccgcca gcacttccgg
    18901 gtccagacca ttagcctctt tttcctgcca gttttgcgtt tctggcggcc aggtatccag
    18961 caaaccaaga aatgccacct gttcgccacg ggcacgcagt cgcgccgcaa tgccctgcgc
    19021 cagcgtaccg ccaagggaat accccagcag gtaataaggg ccgtgcggtt gttgttcaag
    19081 taacgttgcc agatgcgctt cacagacttc atccagattt gccgcggtat gcatggggcc
    19141 attggggcgc ggcgactgaa tgccgataat cgaccattgt ggatcgatat aacgcgagag
    19201 cacgctaaac tgccaggcaa aaccggatgc cggatggaaa caaaacagcg tcgggccatt
    19261 accttcacgc aacggcagaa tggtttcgaa tcccatacgc cgggtgctgt cttcttcagc
    19321 atcaataatc gttgccagtt tagcgacggt tgacgcgacc atcacttgcc ccggcgtcac
    19381 ctggcgggca acctgccgac ttaactgcgc tgccagtttc attgccagta gcgaatgacc
    19441 accaagcgca aagaaatcag catcggcatc ctgcacgtca caacccagca acgacgcgaa
    19501 tgccgcggca ataatcgttt cactgcccgc ttttggcgca cgccccggcg cttgtgcctt
    19561 cagttcaggc aacggtaagg ctttgcgatc cagcttgccg ttggcgctaa gaggtaacag
    19621 tggaagttgc agcagaacca cgggcaccat atgtggtggc aatgtttcgc gaagctgcgc
    19681 ctgtaatgcg ctggtatcca acggcagacc tgattgcgac accagatagc ccaccaactg
    19741 acgcgcatca ccaccggtgg ctgccgcctg gttaatcaca caggcgtggg taacggcttg
    19801 ttcgacatcc ggcagcgcct gcatcacgcg atcgatttcg cccagttcaa tacgctgccc
    19861 gcgaattttt agctgatcgt cactgcgccc gaggtactcc accgcgccgt tatccagcca
    19921 gcgggcaacg tctccggtac ggtacatccg ttcaccaggg acaaaaggat cggcaataaa
    19981 gcggctggcg gtcagatcgg gtcgtccaag atacccctgc gccagttgaa tgccggtgag
    20041 atagagatca cccgccacac ccggcggcac cggatgcatc atcgcatcaa gaatacgcag
    20101 acccgtattc cataccggat aaccaatcgg cacactgctg ccgcgcacct gtgccagttc
    20161 ctcgccaaaa gccggatacc agctgacatc taccgccgct tccgtcgggc catatagatt
    20221 atgcaacggc acgctggtta actgttgcca ttcgcggcat aaatcagccg gtaaggcctc
    20281 accactacag aaaacctgtt tcaacgtcgc gcaactctgg cgagcggttt gcggcgtcag
    20341 cgatgcaaca aatgctgcca gcatcgacgg cacaaagtgc gtggtcgtta cgccatattc
    20401 ggcaaagaat tgctgcatag cgagcgggtc gcggtgcgct tccggttcag ccatcaccag
    20461 tttagccccg gcaataaacg gccagaaaaa ctcccacacc gagacatcaa aactgcacgg
    20521 cgttttttgg gcaacgacat cttcgcctgt aagcagataa tgattttgca tccacaacag
    20581 gcggttaacg atagccgtct gcccgaccat tacccctttc ggcctgccgg tggagccaga
    20641 ggtaaagatg atataagccg tgtgatgcgg ttgtgaaagt tgcagcggcg cactgccctg
    20701 cggtttaagc ggggcgttat agcaaaggtt ggttaaatct ggaacatcgg caaagcgcgg
    20761 cagttgatcg tcggtggtga ttaacagcga cggacgcgca tcttccagca tcattttcag
    20821 gcgatcgtcc ggatagccgg tatccagcgg tagccaggcc gcgccagctt caactatcgc
    20881 atgtagcgcc agggtcaaaa agaccgagcg cggtagcgcc accgccacgc tgtcgcccgg
    20941 tttaacgcca cgctcacgca gcagattcgc cagcgccacc acctgctcgc gcatttcccg
    21001 atagctgaac tggtaacgcg catctgccag cgccggagca tccggtgttt ttgccgcttg
    21061 ttctgccacc agcgcgctca gcgtggtttc tggaatctca acctgagtgg cgttgatctg
    21121 cgccagctgc gcatactcac ctggcagcat aatatcgaca tcgccgcaca acagcgccgg
    21181 atccgcggcg aactgggcaa tcagcatttt caggcgttca gcatgctgga ttaacgttgg
    21241 ctcatcgtaa cgctgtttat tggcgaggat ctcaatactc aaatcaccgt gttcatccgg
    21301 gaacagggcc agttcaaggt cattaaccgg accggttgcc aaggtatggg tttgcgcctg
    21361 aacaccagga atatccagtt ggtaatcaaa taccttgata ttgagtaccg gaccaaacag
    21421 cggttcatca cctgccgctc gcccgctgtc acggacaatt tgttcggcat cgtaacgttg
    21481 atgacgacgc atttttttca gttgtgctgc cagtcgggtt gccagttccg gcagcgtttc
    21541 ttgcgccgca atgtgaatac ccaacggcaa aacgttgagc acgggtccgg tagccgtcag
    21601 cgccgccgag cccagtcgac gcataaagat aaatccagcg gcgtagtcca tgcgattgca
    21661 caatcgcccc agccacaaag ccgtcagcgc aagggctaaa tcggtacgct gcacacctga
    21721 aagttgcgta gccaactggc ggaattcccc gtcggtaaat tccattttca gacgtaaaat
    21781 atctgccgaa gcgctgcgcc ccggtaaagg tgccggagaa agtgacgcgg gcggcggcag
    21841 ttgacgacgc tgttctgccc agaatgccgc atcacgctgc caggcttcgc tttcgcggta
    21901 ttgctggtac tcttccacta catcagcgaa aggcgtaaac ggcgaagcag gcgttggttc
    21961 accacgcagc catgtgcagt aaatattggc gatctggcgg gtaatggccg ggaaactgaa
    22021 gccatcgacc agcaaatggt gataacgctg ataccagtac cagcggttat ccgccacctg
    22081 tatcagctga tgaaagacca gtggtttacc gctatcgacg cgcagatctt gttgcaaatc
    22141 cgcctgcatt aatgcctgcg cagtaccgtg cggatcaatg ttggttcgca ggtcgataat
    22201 ttctggcagt tcgaacgtca gcgcatcatc gacccactgc cagacttcgc cgttatcttc
    22261 cgtaaaacgc atccgcagcg tatctgcttg cgctagtccg tcaaccaccg cgcgggccag
    22321 taatggcgca tcaacctctc cggttaactc aacgtaatgc gctacgctcc aggcggaggg
    22381 taaatccgac agtttttctg ccatccagat gccgggctgt gcggcgacca aaggtaaatg
    22441 ctggctcatt gcgcctcctg caactgggtg aaattcgccg gtgtcagagt acgccagtgg
    22501 gcttcaagcc actgctggca ggactcttgt gactgcggct gacacacaat gtcccagcct
    22561 gccggtaagg cgcattgttg cggccacaga ctgaattgcc cctgcgcatt gcgcaaaatg
    22621 taaaacgctc cctgtggatc atcgaaggga ttactgaatg ccatattcaa ctcctgtcat
    22681 ggaaaagtgg ttgccagagg tcgattagcc cctgcatcaa gccaccgcgc caacaaagcg
    22741 catcatgtcc gccgtcaacc tgacgccaga aaatggattc ttttatgggg tgtaattgcg
    22801 catacagcgc ctgattggcc cgcataatca tcggctcgcg aatacccgct tccagcacaa
    22861 tgcgcagacc ttcggcgcta acttcaccag ctttaagctt ttcaagtaac acgccctctt
    22921 gctgcccgcc ccgatgcggc caccagtacg atcctgactg gcttaataca caaccaaaac
    22981 gttcaggcca gtgcagtccg gcatacagcg cggaaagtcc accaaaactc tgcccggcaa
    23041 ccacggtgcg atcggcgcga tcgctaaaag gggcaatagc tttcaccagg ggtaataact
    23101 cttgctgtac tgcgagccag aaatccgcat tacacggcag ttcgtgagcg cggtgcgtgg
    23161 tgtcgatagc gtcgatcaac acatacacgg cgggaggaag ttgctgacga tgggtcagcg
    23221 aagtcagcac tggccagacg ggcatacttt gcgcccaaaa ttcgccatcg agcaaaactg
    23281 ccagcgggcg ttcttctgct gttgcatcgc cggtggtaaa aatccataca cgacgtgaat
    23341 ttttcaaccg ttcacttttc cagataattt ctttggctgg tatttctggc gcttgcggac
    23401 aatcccatcc cggttgcaga ggcgcttgcg gcatttcgag tgcagaaaca gcgtgccctc
    23461 gcccgccttt ccagctttgt ggattcagcg gatcggctat tgcctggggt aatagttttc
    23521 gccagccttc acgtaattcg aggcgatcgg gggatggtgc agaaaaaatg tcatcgcgtt
    23581 cggtgggaat aaagcagtag ctgccgcgcc agttggcatt gagttgtgtc gtccactgcc
    23641 agacatcagt acctgcaatg cgctgcatcg actggggctg gctgttctga tgatgatcgg
    23701 tcacaccagt gatgtagacc catacgcgct ttatcggcga gtattcttca gaaccttggg
    23761 gatcacgcca ccagaaagtg acctcaaaca tttcgtcatt cagacgctgc cattccgggc
    23821 catgtttcga ctgccaccag ctctcacttc ctacttttaa cgccgtcaca ccataacccc
    23881 atgtttactg tgcaattttt cattgattgc agaaatatat tgataatatt attgataact
    23941 atttgcattt gcaatagcgt aatggcgcgc cgtgggaagc gcggacatta attaaccaac
    24001 tgcactgcgt gtctttcagg atcaaaggtt ttcgcggtag cgggatgcgt cgtgttgagg
    24061 acgaccatgc ccgacagttg caattcgtgg caaaaatgca ggaataaaac aatgaacaag
    24121 aagattcatt ccctggcctt gttggtcaat ctggggattt atggggtagc gcaggcacaa
    24181 gagccgaccg atactcctgt ttcacatgac gataccattg tcgttaccgc cgccgagcag
    24241 aacttgcagg cacctggcgt ttcgaccatc accgcagatg aaatccgcaa aaacccggtt
    24301 gcccgcgatg tgtcggagat catccgtacc atgccaggcg ttaacctgac cggtaactcc
    24361 accagtggtc agcgtgggaa taaccgacag attgatattc gcggtatggg tccggaaaac
    24421 acgctgattt taattgacgg caaaccggtc agcagccgta actcggtgcg tcagggctgg
    24481 cgtggcgagc gcgatacccg tggtgatact tcctgggtgc cgcctgaaat gattgaacgt
    24541 attgaagttc tgcgtggtcc ggctgcagcg cgttatggca acggcgcggc gggtggcgta
    24601 gttaacatca ttaccaaaaa aggcagcggt gagtggcacg gttcctggga cgcttatttc
    24661 aatgcgccag aacataaaga ggaaggtgcc actaaacgca ccaacttcag tctgaccggc
    24721 ccgctgggcg acgaattcag cttccgcttg tatggcaacc tcgacaaaac ccaggctgac
    24781 gcgtgggata tcaaccaggg ccatcagtcc gcgcgtgccg gaacgtatgc cacgacgtta
    24841 ccagccgggc gcgaaggggt gatcaacaaa gatattaatg gcgtggtgcg ctgggacttc
    24901 gcgcctctgc aatcactcga actggaagcg ggttacagcc gccagggtaa cctgtatgcg
    24961 ggcgataccc agaacaccaa ctctgacgct tacactcgct cgaaatatgg cgatgaaacc
    25021 aaccgcctgt atcgccagaa ctactcgctg acctggaacg gtggctggga taacggagtg
    25081 accaccagca actgggtgca gtatgaacac acccgtaact cgcgtattcc ggaaggtctg
    25141 gcgggcggta ccgaagggaa atttaacgaa aaagcgacac aggatttcgt cgatatcgat
    25201 cttgatgacg tgatgctgca cagcgaagtt aacctgccga ttgatttcct cgttaaccaa
    25261 acgctgacgc tgggtacgga gtggaatcag caacggatga aggacttgag ttccaatacc
    25321 caggcgctga ccggggcgaa caccggcggc gctattgatg gtgtgagtgc caccgaccgt
    25381 agcccgtatt caaaagcaga aattttctcg ctatttgccg aaaacaacat ggagctgact
    25441 gacagcacca tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt cggcaataac
    25501 tggagcccgg cgctgaacat atcgcaaggt ttaggcgatg acttcacgct gaaaatgggc
    25561 attgcccgcg cctataaagc gccgagcctg taccagacta acccgaacta cattctctac
    25621 agtaaaggtc agggctgcta tgccagcgcg ggcggctgct atctgcaagg taacgatgac
    25681 ctgaaagcag aaaccagcat caacaaagag attggtctgg agttcaaacg cgacggctgg
    25741 ctggcgggcg tcacctggtt ccgtaacgat tatcgcaata agattgaagc gggctatgtg
    25801 gctgtagggc aaaacgcagt cggcaccgat ctctatcagt gggataacgt gccgaaagca
    25861 gtggttgaag gtctggaagg atcgttaaac gtaccagtta gcgaaacggt gatgtggacc
    25921 aataacatca cttatatgct gaagagtgaa aacaaaacca cgggcgaccg tttgtcgatc
    25981 atcccggagt acacgttgaa ctcaacgctg agctggcagg cgcgggaaga tttgtcgatg
    26041 caaacgacct tcacctggta cggtaagcag cagccgaaaa aatacaacta caaaggtcag
    26101 ccagcggttg gcccggaaac caaagaaatc agtccttaca gcattgtggg tctgagcgcg
    26161 acctgggatg tgacgaagaa tgtcagtctg accggcggcg tggacaacct gttcgacaaa
    26221 cgtttgtggc gtgcgggtaa tgcccagacc acgggcgatc tggcaggggc caactatatc
    26281 gcgggtgccg gggcgtatac ctataacgag ccgggacgta cgtggtacat gagcgtaaac
    26341 actcacttct gatgctaacg tcagattgtt gataaagtgc gcgtcgttca tgccggatgt
    26401 ggcgtgaacg ccttatccgg cctacaaaaa tcttgccaat tcaatatatt gcaggaccat
    26461 gtaggcctga taagcgtagc gcatcaggca gtttagcgtt tgtcaccagt ctctaatatg
    26521 gtcgacatga aaactacgca tacctccctc ccctttgccg gacatacgct gcattttgtt
    26581 gagttcgatc cggcgaattt ttgtgagcag gatttactct ggctgccgca ttacgcgcaa
    26641 ctacaacacg ctggacggaa gcgcaaagcc gaacatttag ccgggcggat cgctgcaatt
    26701 tatgcgctgc gggaatatgg ctataaatgt gtgcccgcaa tcggcgagct acgccaaccg
    26761 gtgtggcctg cggaggtata cggcagtatt agccactgcg gggctacggc attggccgtg
    26821 gtttcccgtc aaccgattgg cgttgatatc gaagaaattt tttctgcgca aacagcgacc
    26881 gaattgacag acaacattat tacaccagca gaacacgagc gactcgcaga ctgcggttta
    26941 gccttttctc tggcgctgac actggcattt tccgccaaag agagcgcatt taaggcaagt
    27001 gagatccaaa ctgatgcagg ttttctggac tatcagataa ttagctggaa taaacagcag
    27061 gtcatcattc atcgtgagaa tgagatgttt gctgtgcact ggcagataaa agaaaaaata
    27121 gtcataactc tgtgccaaca cgattaattg acaacatctg gtacgattcg cccgcagcca
    27181 tcactgaccg cgggcgaaag tgtaaagcag gtgccttacc atcctgacct gacaaccgga
    27241 tatgcgggga ttgctccccg cacaacggct acttcttcgg ttcgtaagcg agaacagcct
    27301 taaactcaat attacgttcc tttacggtga actcacacag cgaatccccg accagaagcg
    27361 taaatcccag caccgttaaa cacagcacta tgactgccgc aagggcatat ttcgtcagca
    27421 tgtttatatt gcctccatgc gaagaaggga ctaccatcca aattgttcag gtttgggtag
    27481 gtagccccag ggttgataga aatatcgtcc tggggcttca tctttccaga cctcagagta
    27541 aacctgaaac cggaaagctt caggcacccg cagttatctt acccttgctt agcaaaatag
    27601 ctgtttattt atacagtaag tcctgtttcg gacttctcac tccccatttg atcaactgaa
    27661 gctgaacccg tttttttctg catgcctcaa tcgtcaaaat tcttcgccac cagtcagcac
    27721 tgccataact tgtcacattg caaaagtagc caaattgctt ctgtcataat cagttcacca
    27781 tcaccaaaaa aaagaaacat tccagaactt ccgcatctcg cccagccgct cccacttcta
    27841 tactgaatag aaaacgccaa cataagagaa acctatgcca ttacccgatt ttcatgtttc
    27901 tgaacctttt acactcggta ttgaactgga aatgcaggtg gttaatccgc cgggctatga
    27961 cttaagccag gactcttcaa tactgattga cgcggttaaa aataagatca cggccggaga
    28021 ggtaaagcac gatatcaccg aaagtatgct ggagctggcg acggatgttt gccgtgatat
    28081 caaccaggct gccgggcaat tttcagcgat gcagaaagtc gtattgcagg cagccgcaga
    28141 ccatcatctg gaaatttgcg gcggtggcac acacccgttt cagaaatggc agcgacagga
    28201 ggtatgcgat aacgagcgct atcaacgaac gctggaaaac tttggttatc tcattcaaca
    28261 ggtgaccgtt tttggtcagc atgttcatgt tggctgcgcc agtggcgatg acgccattta
    28321 tttgctgcac ggcctgtcac ggtttgtgcc gcacttcatc gccctttccg ccgcgtcgcc
    28381 atatatgcaa ggaacggata cgcgttttgc ctcctcacga ccgaatattt tttccgcctt
    28441 tcctgataat ggcccgatgc cgtgggtcag taactggcaa caatttgaag ccctgtttcg
    28501 ctgtctgagt tacaccacga tgatcgacag cattaaagat ctgcactggg atattcgccc
    28561 cagtcctcat tttggcacgg tggaggttcg ggtgatggat accccgttaa cccttagcca
    28621 cgcggtaaat atggcgggat taattcaggc taccgcccac tggttactga cagaacgccc
    28681 gttcaaacat caggagaaag attacctgct gtataaattc aaccgtttcc aggcctgtcg
    28741 ctatgggctg gaaggcgtca ttaccgatcc gcacactggc gatcgtcgac cgctaacgga
    28801 agacaccttg cgattgctgg aaaaaatcgc cccttccgca cataaaattg gtgcatcgag
    28861 cgcgattgag gccctgcatc gccaggtcgt cagcggtctg aatgaagcgc agctgatgcg
    28921 cgatttcgtc gccgatggtg gctcgctgat tgggctggtg aaaaagcatt gtgagatctg
    28981 ggccggtgac taagtaggga attgccttac agaagaatat ccctgacaat ggtgttttaa
    29041 cttacattta acaatgttat gaaacaccct ttagaaacct tgactaccgc agcaggcatt
    29101 ttgctgatgg ctttcctctc ttgcctgctg ctgcccaccc ccgcactggg gctgacgctg
    29161 gcacaaaaac tggtgaccat gttccatctg atggatctta gccagcttta cactttattg
    29221 ttttgtctgt ggtttttagt gctgggcgct attgagtatt ttgttctgcg ctttatctgg
    29281 cgacgctggt tctcgctggc ggattaaacg tcgattatcg ccgtacttcg cggcatactt
    29341 tgcttattct ccttcgccta aaggaacgtt tatggataag caatcactgc acgaaacggc
    29401 gaaacgcctg gcccttgagt taccctttgt cgagctttgc tggccttttg gcccggagtt
    29461 cgatgttttt aaaattggcg gcaaaatttt tatgctgtcg tcggagctgc gcggcgtccc
    29521 ctttatcaat ctgaagtccg atccacaaaa atccctgtta aatcagcaga tatacccgag
    29581 cattaagcca gggtatcaca tgaataaaaa gcactggatt tcggtgtatc ccggcgagga
    29641 aatctccgaa gcgttacttc gcgatctgat caacgattcg tggaatctgg tggttgatgg
    29701 tctggctaaa cgcgatcaaa aaagagtgcg tccaggctaa agcggaaatc tatagcgcat
    29761 ttttctcgct taccatttct cgttgaacct tgtaatctgc tggcacgcaa aattactttc
    29821 acatggagtc tttatggata tcatttctgt cgccttaaag cgtcattcca ctaaggcatt
    29881 tgatgccagt aaaaaactta ccccggaaca ggccgagcag atcaaaactc tcctgcaata
    29941 cagcccatcc agcaccaact cccagccgtg gcattttatt gttgccagca cggaagaagg
    30001 taaagcgcgt gttgccaaat ccgctgccgg taattatgtg ttcaacgaac gtaaaatact
    30061 tgatgcctcg cacgtcgtgg tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa
    30121 gctggttgtt gaccaggaag atgctgatgg ccgctttgcc acgccggaag cgaaagccgc
    30181 gaacgataaa ggtcgcaagt tcttcgctga tatgcaccgt aaagatctgc atgatgatgc
    30241 agagtggatg gcaaaacagg tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc
    30301 tctgggtctg gacgcggtac ccatcgaagg ttttgacgcc gccatcctcg atgcagaatt
    30361 tggtctgaaa gagaaaggct acaccagtct ggtggttgtt ccggtaggtc atcacagcgt
    30421 tgaagatttt aacgctacgc tgccgaaatc tcgtctgccg caaaacatca ccttaaccga
    30481 agtgtaattc tctcttgccg ggcatctgcc cggctatttc ccctcagatt ctcctgattt
    30541 gcataaccct gtttcagccg tcatcatagg ctgctgttgt ataaaggaga cgttatgcag
    30601 gatttaatat cccaggttgg ggatttagcg ggtattgaga tcgatcacac cacctcgatg
    30661 gtgatgattt tcggtattat ttttctgacc gccgtcgtgg tgcatattat tttgcattgg
    30721 gtggtactgc ggaccttcga aaaacgtgcc atcgccagtt cacggctttg gttgcaaatc
    30781 attacccaga ataaactctt ccaccgttta gcttttaccc tgcaggggat tatcgtcaat
    30841 attcaggcgg tattctggct gcaaaaaggc accgaagcgg cagatattct gactacctgc
    30901 gcgcagttgt ggatcatgat gtatgcgctg ctttcagtct tctcgttgct ggatgttatt
    30961 ttgaatctgg cgcagaaatt cccggcggca tctcagttac cgctgaaagg gatatttcag
    31021 gggattaaac tgatcggcgc aatcctggtc ggcattttga tgatctcgct gctgattggt
    31081 cagtcgccag cgattctgat cagcggtctt ggtgcaatgg ctgccgtgct gatgttggta
    31141 tttaaagatc cgattcttgg tctggtggca ggtattcagc tttccgcgaa cgatatgctg
    31201 aaactgggcg actggctgga gatgccgaaa tacggcgcgg atggcgcggt gatcgatatt
    31261 gggttaacca ccgtcaaagt gcgtaactgg gacaatacca ttaccactat tcccacctgg
    31321 tctctggttt ctgactcctt taaaaactgg agcgggatgt cagcatctgg cgggcgacgt
    31381 attaagcgca gtatcagtat tgatgtcacc agtattcgtt ttcttgatga agacgaaatg
    31441 caacgtctga ataaagcgca cttgttaaaa ccttatttaa ccagccgcca tcaggaaatt
    31501 aatgagtgga atcgccagca aggttctacg gagtcgatat taaacctgcg ccgaatgacc
    31561 aatattggaa cctttcgtgc ctatctgaac gaatatctgc gtaaccatcc gcggattcgt
    31621 aaagatatga ccttaatggt acgccaactg gctccaggtg ataacggttt accgctcgag
    31681 atctacgcgt ttaccaacac cgtggtgtgg ctggaatatg aaagcattca ggctgatata
    31741 ttcgatcaca tatttgcgat tgtcgaagag tttggtctgc gacttcatca gtcgccagcc
    31801 ggcaatgata ttcgctctct ggcgggtgca tttaagcagt aattaaaaaa accgctctca
    31861 tcgaatggat gagagcggac atcgttttgt cggatgtagc gtgaatgcct tatttccgac
    31921 gcagcgtttt aaatgccaca aacaggaata caatccacac cggcagcagg atcgctgaca
    31981 agcgcatatc atccatcgtg cacatcagca gcaaaatcat gccgaggaag gcaatgcaaa
    32041 gatagttgcc gaacggataa agcagcgcct taaactgtgt ttcacgtccc tgacgtcgca
    32101 tcgccgcgcg aaaacgcaga tgcgccaggc agatcataat ccagttcaac agcagcgttg
    32161 ctaccaccag cgccatcagc agaccaaacg ctttttgcgg cagcagatag ttgattaaca
    32221 ccaccagcga agtgatcgct ccggaaagca tcagcgagtt aatcggcacg ccgcgatggc
    32281 tgacgcgagt caaaaacttc ggcgcattac cctgcacaga aaggccaaac agcatgcggc
    32341 tgttagagta aaccccgctg ttatacactg acagcgatgc taccagaatg acgaagttca
    32401 gcgcagaagc tacctcgttg ctgtcgagat tatggaaaat catcacaaac gggctactgt
    32461 tggatttcac ttccacccac ggatagagcg ccagtaaaac caccagtgaa cagatgtaaa
    32521 acagcaggat gcgatacacc acctgattta ccgcttttgg aatgcttttt tccggatcgc
    32581 gcgcttcagc ggcagtaatc ccaatcagct ccagaccgcc gaaggagaac ataattaccg
    32641 ccagcgacaa aatcagccca ttccagccgg tggcgaagaa accaccgtag cgccagaggt
    32701 tgtcgatact ggctttctcg ccgccgtgac cagaaaacag cagccacagg ccaaagccga
    32761 tcataccgat gattgccagc actttaatca acgcaaacca gaactcggtt tcgccatata
    32821 agcgcacgtt caccaggtta acggcgttga tgataataaa gaaggcggca gcccaaatcc
    32881 acgttggaac atccgggaac cagtactgca tatagatgcc cgcagcggtc agctctgcca
    32941 ttcccaccag cacgaacatt acccagtagt tccagccaga gaggaagccc gcaaacggtc
    33001 cccagtattt ataggcaaag tgggcaaatg aaccggatac cggctcttcg accaccatct
    33061 cgccgagctg gcgcataatc aggaaagcga tgatcccggc gacgccgtag cccagcaata
    33121 cagccggacc cgccatctga atcgccgggc caatgccaag aaacagacca gtaccaattg
    33181 cgccacccag cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga agcgtcggct
    33241 cttgatttga cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg
    33301 tgtctttttt gttgaggggc accttttaac atttagtgcc catcgtcgca agacacaatc
    33361 cacacggtta aacggggtat cctgctttta tttccgtacc cgatgtcggt gcagccacat
    33421 cagcttatac gccgccggga taataaacag cgacagcaaa ggcgcggtga tcatgccgcc
    33481 aatcatcggt gcggcaatcc ggctcatcac ctctgaacca gcccccgttc cccacagaat
    33541 cggcagcaga ccggcgataa tcaccgccac cgtcatcgct ttcgggcgca cgcgcagtac
    33601 cgcgccgtga tacaacgcct catccagctt ctgctcactg aatgtttgcg gattattcaa
    33661 cgacggctcg gcctctatgg cgtgacgtaa atacatcagc atcaccacgc caaattcggc
    33721 ggcgcccccg gcaagggcaa taaaaccggt gcccgttgcc acagaaagat gaaagcccat
    33781 ccaccacagc aaccagatgc cgcccaccag cgcaaacggt acgctgctga taatcagcaa
    33841 cgcttcgccc acccgacgga atgccagata caacagcacg aagatgatca tcagtgtcat
    33901 cggcaccatg agtttcagct tatggttggc gcgctccagt agctcgaact gcccggagaa
    33961 tgccacgctg gtgcccggtt ttaactgcac tttttcaact atcgcttttt gcaaatcgtg
    34021 aaccaccgac accatgtcac gatcgcgggc atcgatataa atccagctcg tcgggcgcgc
    34081 attctcggtt ttcagcatcg acggtccggt agagacttta acgtcggcca cgtctgccag
    34141 ggtgatttgc tgcttcatcg gcgtcaggat cggtagctgg cgcagtgcct gctttgcgga
    34201 taacgcagat taatgggata acgggcaatc ccttccaccg tttcgccaac catcgccccg
    34261 cccaccgcag aagtcacaaa caactgcaca tccgccaccg tcataccgta acgcgcggct
    34321 ttttcacggt taatctcaac gttgatatag cgcccacctt ccagacgctc ggcaagcgca
    34381 gaagccacgc ctggcaccgt tcgcgctact tcttcaattt gctcagccat cgcgtcgata
    34441 tccgccagca cagtgccgga aactttaatg ccgatggggc ttttaatgcc ggttgagagc
    34501 atatcgatac ggttacgaat tggcggcacc cacagattcg ccagccccgg cagccgcacg
    34561 gtgttatcca gttcctcaat gattttgtcc atcgtcatgc caggccgcca ctgatcctgc
    34621 ggcttaagct ggatggtcgt ttcaaccatc tcgagcggag cagaatcggt ggcggtttcc
    34681 gctttcccgg ttttgccaaa tacccgcgcc acttcaggta cgctcataat tagcttgtcg
    34741 tttttttgca gcatactcgc cgcctctgct gcggaaatcc ccggcagcgt cgatggcata
    34801 tacaacaagt cgccttcatt gatctgcggt aaaaattccc cgccaacttt attgagcggc
    34861 cagagaaccg tcagcaccga aagcgctgcc accaacagcg tggttttcgg ccagtgcagt
    34921 actttcagca atagaggatg ataaacacga atcaaaaagc gattgagcgg gttactgctt
    34981 tccggcggaa ttttgccacg gatccagtaa cccatcagga tcgggatcac tacgatcgcc
    35041 agcagcgccg cacccgccat cgcatacgtt ttggtgaacg ccagcgggcc aaacagacgc
    35101 ccttcttgcc cttccagggt gaagattggg ataaacgaca gcgtgataat cagcagactg
    35161 ataaacagcg ccggccccac ttcaacagat gcatcggtaa tcacctgcca gcgcgtttta
    35221 ttatccagcg tggcgtcagg atgctggtgc tgccactctt ccagccgttt atgcgcattc
    35281 tcgatcatga cgatagcagc atcgaccatc gccccgacgg caatcgcaat gccacccaac
    35341 gacataatat tggcattcag tccctggaag ggcatgacaa taaaagcaat acacaacccc
    35401 agcggcaacg aaataatcgc caccagcgcc gagcgcacat gccagagaaa cagcgcacag
    35461 actaccgcca ccacaataaa ctcttccagc aacttgccgc tgaggttgtc gatggcgcgg
    35521 tcaatgagct ggctgcgatc gtatgttgta actatctcca cgccttccgg cagactactt
    35581 ttcagcgttt ccagtttgtc cttcacggcg gcgatcactt ctcgggcgtt tttaccggag
    35641 cgcaggatca ccaccccgcc cgccacttcg ccttcgccgt tcagttcggc aatgccccgg
    35701 cgcatctccg ggccaacctg gaccttcgca acatcgcgca gataaacggg cacgccattt
    35761 tcactggctt ttaaaacgat gtgattaaag tcgtcgagcg tttgcagata gccgctggcg
    35821 cgcaccatat attccgcttc cgccagttcg atcgacgaac cgcccgcttc ctggtttgaa
    35881 gcatccagcg cgctttttac ttcggcgaga ctgatgccat actgcgccag gcgctgggga
    35941 tcgataacca cctgatactc tttcaccaca ccgcccaccg acgccacttc cgcaacgtca
    36001 gggatggttt tcagctcata tttgagaaac cagtcctgta atgagcgtaa atcggccaga
    36061 tcgtgcttac cgctgcgatc caccagtgca tattcataga tccagccaac acccgtggca
    36121 tctggcccca gctcggcgct gactcccgca ggcagcttac cctgtacctg gttgaggtac
    36181 tccagcaccc gcgaacgcgc ccagtacgga tcggtaccat cttcgaaaat gacatacaca
    36241 taagagtcgc caaactgcga gaaaccacgc acagtcttcg cgccaggcac cgacaacatg
    36301 gtggtggtta gcggataagt cacctgattt tcaacgattt gcggtgcctg cccgggatag
    36361 ctggttttaa taatcacctg cacatcggag agatccggca gcgcatccac tggcgtatta
    36421 atgatggtcc aggtgcccca gatgctaaga aacaacgcgc ccatcagcac caaaaaacgg
    36481 ttcgccaccg agcgacgaat aatccattca atcattggtt attccctcaa tgcgcatggg
    36541 tagcactttc agagcgcatc cgctccagtg cgccagaaat attggc
//
LOCUS       DAJTNN010000018        36520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTNN010000018 DAJTNN010000000
VERSION     DAJTNN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.018"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..896
                     /locus_tag="OO150_000817"
     CDS             <1..896
                     /locus_tag="OO150_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5515055.1"
                     /translation="DIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEEQ
                     IGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNTY
                     PTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELENL
                     TISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGINP
                     TIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIR
                     NHRYTATMLQHIAEDRDGTNH"
     gene            complement(893..2370)
                     /gene="scpC"
                     /locus_tag="OO150_000818"
                     /pseudo
     CDS             complement(893..2370)
                     /gene="scpC"
                     /locus_tag="OO150_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(2394..3179)
                     /gene="scpB"
                     /locus_tag="OO150_000819"
     CDS             complement(2394..3179)
                     /gene="scpB"
                     /locus_tag="OO150_000819"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5515056.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(3190..4185)
                     /gene="meaB"
                     /locus_tag="OO150_000820"
     CDS             complement(3190..4185)
                     /gene="meaB"
                     /locus_tag="OO150_000820"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5515057.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKRTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(4178..6322)
                     /gene="scpA"
                     /locus_tag="OO150_000821"
                     /pseudo
     CDS             complement(4178..6322)
                     /gene="scpA"
                     /locus_tag="OO150_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(6526..7419)
                     /gene="argP"
                     /locus_tag="OO150_000822"
     CDS             complement(6526..7419)
                     /gene="argP"
                     /locus_tag="OO150_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5515058.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7802..8461
                     /gene="rpiA"
                     /locus_tag="OO150_000823"
     CDS             7802..8461
                     /gene="rpiA"
                     /locus_tag="OO150_000823"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5515059.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8716..9948
                     /gene="serA"
                     /locus_tag="OO150_000824"
     CDS             8716..9948
                     /gene="serA"
                     /locus_tag="OO150_000824"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5515060.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            10177..10236
                     /gene="ibsC"
                     /locus_tag="OO150_000825"
     CDS             10177..10236
                     /gene="ibsC"
                     /locus_tag="OO150_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5515061.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10569..10628
                     /locus_tag="OO150_000826"
     CDS             10569..10628
                     /locus_tag="OO150_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5515062.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10729..11331)
                     /locus_tag="OO150_000827"
     CDS             complement(10729..11331)
                     /locus_tag="OO150_000827"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5515063.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHELKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11352..11535)
                     /gene="ssrS"
                     /locus_tag="OO150_000828"
     ncRNA           complement(11352..11535)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO150_000828"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11577..11906)
                     /gene="zapA"
                     /locus_tag="OO150_000829"
     CDS             complement(11577..11906)
                     /gene="zapA"
                     /locus_tag="OO150_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5515064.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            12074..12652
                     /gene="ygfB"
                     /locus_tag="OO150_000830"
     CDS             12074..12652
                     /gene="ygfB"
                     /locus_tag="OO150_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5515065.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12678..14003
                     /gene="pepP"
                     /locus_tag="OO150_000831"
     CDS             12678..14003
                     /gene="pepP"
                     /locus_tag="OO150_000831"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5515066.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            14000..15178
                     /gene="ubiH"
                     /locus_tag="OO150_000832"
                     /gene_synonym="visB"
     CDS             14000..15178
                     /gene="ubiH"
                     /locus_tag="OO150_000832"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5515067.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            15201..16403
                     /gene="ubiI"
                     /locus_tag="OO150_000833"
                     /gene_synonym="visC"
     CDS             15201..16403
                     /gene="ubiI"
                     /locus_tag="OO150_000833"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5515068.1"
                     /translation="MQCVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16477..16560)
                     /locus_tag="OO150_000834"
     CDS             complement(16477..16560)
                     /locus_tag="OO150_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515069.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16850..17944
                     /gene="gcvT"
                     /locus_tag="OO150_000835"
     CDS             16850..17944
                     /gene="gcvT"
                     /locus_tag="OO150_000835"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5515070.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17968..18357
                     /gene="gcvH"
                     /locus_tag="OO150_000836"
     CDS             17968..18357
                     /gene="gcvH"
                     /locus_tag="OO150_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5515071.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18476..21349
                     /gene="gcvP"
                     /locus_tag="OO150_000837"
     CDS             18476..21349
                     /gene="gcvP"
                     /locus_tag="OO150_000837"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5515072.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYL"
     gene            21615..22358
                     /locus_tag="OO150_000838"
                     /pseudo
     CDS             21615..22358
                     /locus_tag="OO150_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(22415..23848)
                     /gene="bglA"
                     /locus_tag="OO150_000839"
     CDS             complement(22415..23848)
                     /gene="bglA"
                     /locus_tag="OO150_000839"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5515073.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23893..24204
                     /locus_tag="OO150_000840"
     CDS             23893..24204
                     /locus_tag="OO150_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR5515074.1"
                     /translation="MQPNDITFSQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24368..25027
                     /locus_tag="OO150_000841"
     CDS             24368..25027
                     /locus_tag="OO150_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5515075.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSMCHFLAIYLYIGQ
                     A"
     gene            complement(25223..26203)
                     /gene="ygfZ"
                     /locus_tag="OO150_000842"
     CDS             complement(25223..26203)
                     /gene="ygfZ"
                     /locus_tag="OO150_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5515076.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAADDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26446..26712
                     /gene="sdhE"
                     /locus_tag="OO150_000843"
     CDS             26446..26712
                     /gene="sdhE"
                     /locus_tag="OO150_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5515077.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26693..27100
                     /locus_tag="OO150_000844"
     CDS             26693..27100
                     /locus_tag="OO150_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR5515078.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(27140..27661)
                     /gene="fldB"
                     /locus_tag="OO150_000845"
     CDS             complement(27140..27661)
                     /gene="fldB"
                     /locus_tag="OO150_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5515079.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27773..28669
                     /gene="xerD"
                     /locus_tag="OO150_000846"
     CDS             27773..28669
                     /gene="xerD"
                     /locus_tag="OO150_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5515080.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28694..29404
                     /gene="dsbC"
                     /locus_tag="OO150_000847"
     CDS             28694..29404
                     /gene="dsbC"
                     /locus_tag="OO150_000847"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5515081.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            29410..31143
                     /gene="recJ"
                     /locus_tag="OO150_000848"
     CDS             29410..31143
                     /gene="recJ"
                     /locus_tag="OO150_000848"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5515082.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31234..32332
                     /gene="prfB"
                     /locus_tag="OO150_000849"
     CDS             join(31234..31308,31310..32332)
                     /gene="prfB"
                     /locus_tag="OO150_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5515083.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32342..33859
                     /gene="lysS"
                     /locus_tag="OO150_000850"
     CDS             32342..33859
                     /gene="lysS"
                     /locus_tag="OO150_000850"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5515084.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33902..34450)
                     /gene="idi"
                     /locus_tag="OO150_000851"
     CDS             complement(33902..34450)
                     /gene="idi"
                     /locus_tag="OO150_000851"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5515085.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34505..34576
                     /locus_tag="OO150_000852"
     CDS             34505..34576
                     /locus_tag="OO150_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515086.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34573..34698)
                     /locus_tag="OO150_000853"
     CDS             complement(34573..34698)
                     /locus_tag="OO150_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515087.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34700..36148)
                     /gene="uacT"
                     /locus_tag="OO150_000854"
     CDS             complement(34700..36148)
                     /gene="uacT"
                     /locus_tag="OO150_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5515088.1"
                     /translation="MSAIDSQLLSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSVGL
                     SIIQVGIDWAAGGKGNPQYGNPVYSGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDALWFAIVTPMSFGMPIIDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         8794 a         9194 c         9766 g         8766 t
ORIGIN      
        1 tggacatttt tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata
       61 ttgcccgggc ggcagaaaaa atccatatga cggcttcgcc atttggtaaa agcattgccg
      121 cgctggaaga acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca
      181 ataaagccgg tcaggaactg tatcaaaaac tgttcccggt ttatcaacga ctttctgcga
      241 ttgataatga aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata
      301 atacttatcc gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg
      361 tgaccgccca gccagttgag ttcagcgaaa acggcgttat cgataatctt tttgaccgtc
      421 agttagattt tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc
      481 tgactatcag tgaattacca ccgctgcgat tgggctttct cgtttctcgc cgttatgaag
      541 agcgtcaaga gcaagagctg cttcaggagc tgccctggct acaaatgcgc tttcaaaacc
      601 gggctaactt cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatcccacca
      661 ttatttatcg tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc
      721 tgacggtgat cccgcatttt gcctggcgac tggtgaatcc ggcaacgctg aaatattttg
      781 atgcgccgca caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt
      841 ataccgccac gatgcttcag catattgctg aagatcgtga cgggacaaac cattaaccca
      901 gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt
      961 gaatatgtcc gccgggcgca ttttccagat agcgatgcag ataatcccga tacataggat
     1021 gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg
     1081 cgatcccttg ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc
     1141 acattggcac gacggttgag atcttgcctt ctttagcaat cgacggagcc ataaagatcg
     1201 acagatacgc gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc
     1261 cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa ctccaggccg acgttcagag
     1321 cgatgacgcc cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca
     1381 caatgcggct ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga
     1441 ttgtcaggct ggaggcgctg gccccgctga ttttgtcggt ttccagtaaa tgcaccaccg
     1501 attcctgtag cacttccgaa tacatcatga acggaggaat ttccgggttt tcccccagac
     1561 gcgccattac cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg
     1621 gaatacgcca tgcgccattt cctgcaataa gaacgtgacc acgttatcgg caatctgctg
     1681 gcacatggga ttttgcttat ccagcatatt accggcgtcg ggcaagttgg tttccacgac
     1741 ggcgacaatc tttttcggat cgatttgcac atagcgggta ccgacgcgat ccattgcatg
     1801 gaagatcgac acgctattgc gccgcggtgg cgcgccagga atcacaatat ctgccagttc
     1861 tgcaacgcgc ggatcgtgat agtgattaag ttcaatgatc actttcttcg ctcgcagcag
     1921 ccaggtcggc gcattaccga tcccgctggt taaccagact cgaccatccg gtgccagtgc
     1981 cgatgcttca atgacggcaa catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc
     2041 cacttcgctc aaatgcaggt caacgaaact caccgcgccc tgattgatct ttttacgtaa
     2101 accggacgat gtttgatatg gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc
     2161 gtcagcggcg gcgctgattg acgcccccgt cagcaggcga atttgatacg gctttttggc
     2221 ctcatgctgt tcgttagctc tgcgggcaat cgcggtgggt agggctttcg gcgaacccgc
     2281 cggggtaaag ccgctaaatg ccaccatgtc gttatgctgg ataatttctg ccgcttcatc
     2341 ggcggtcatc cttgtccact gagtttccat ttttactcct tcgttcgcag ggattaatga
     2401 ccaacgaaat taggtttacg tttttcgagg aaagcgttca ttccttcctg gtaatcttcg
     2461 ctgtcataaa ccgcgcgtcg catcccctga atacgttcaa attcatcgga gttcatggtg
     2521 tgcgcttcgc ccagtacacg cagctcttct ttgataacag caatggctaa cggcgctttc
     2581 tcagagatgt ggtgcgccat ttgtaaggtg aaatcttcca gttcttccac ttccacaaca
     2641 tggttgagga tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc
     2701 agctctttga caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta
     2761 tacgggacgc cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc
     2821 agatcggaac tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa
     2881 atgatcggtt tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag
     2941 gagagcggat cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact
     3001 ttggatccac tcggcgcgcg caaaatgata cagcgaattt ccggccggtt gagatcgctt
     3061 aacgcctgca taagatcatc aataaagact ttacttaagg cattaagttt tcggccatag
     3121 ttaaactcaa tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata
     3181 aaaattcctt taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt
     3241 gcgcggtgag agcgtattgt ttttgaccgc taaaagcgtc tggcggtaat agcgatcgaa
     3301 atcttcattc gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc
     3361 caccgattgt tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa
     3421 ctcgatgatg gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag
     3481 aacccgtggc tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat
     3541 atgccgggca atggcgacat tggtatggtt atcgccatcg tctttgttga taacgatcag
     3601 atcagccact tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat
     3661 ttgcaacgag ataaaacagt ccaccatgcg ggcgacttct gtttccgact gcccgacgcc
     3721 aaccgtttcg acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg
     3781 cgctcgctga ctggcaccgc ccagatgacc ggaggatggt accgggcgaa taaacgccgc
     3841 ttcggcacgc gccaggtcat tcatgcggat tttatccccg agaatgctac cgccagtgac
     3901 cgggctgctg ggatcgaccg caataaccgc gacctttaat ccctctcgaa tcaacaacat
     3961 gccaaaggcc tcaagaaagg ttcttttccc cgcgctgggg gtgccggtaa cgcccagtcg
     4021 cagggtgtta ccgcagtacg gcataatggc atcaagcagc tgcgtactta gtgcctgatg
     4081 acgcgggtga cggctttcca ccagcgtcat ggcctgggcg agtgtggcac gctcaccctg
     4141 acgtaagcgg cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca
     4201 gattcagtac gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca
     4261 cgccgcgctc ttgcaggaag gcgtaatcct gcggtggaat gacgccaccg gcgactacgc
     4321 agatatcttc gcgtccccat tttttcagcg cctcgaccag ttccgggatc agcgttttat
     4381 gaccggcagc cagtgaggat gcgcccacta cgtgaacgtc attttctact gccaggcggg
     4441 cgatctcttc aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat
     4501 aggcgctggc gatcactttc gagccacgat cgtgcccatc ctggcccatt ttggcgatca
     4561 gaatgcgcag gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat
     4621 cgaactcgga ggccgatttc tcagattgat gatagctttg cgcaatcacg ccggtaacac
     4681 actggcttgg caccagataa cggtcgaaag cggcttccag cgcatcggaa atttcaccca
     4741 gggtggcgcg aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg
     4801 cggcgtgagt cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa
     4861 tgcgttccag cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat
     4921 cggtttcgtc ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgtttgccct
     4981 ggtcgataag cgactgttcg cgcgctgagg cctattcgat cattcgtttt ggcagacctg
     5041 cttcgatcgc tttcgccata ccaccggctt cgtcgatctg ttggataata gctctggctt
     5101 gtttgacgat ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg
     5161 tgcggcagag ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg
     5221 agaaatcggt aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac
     5281 cgcccagcgt cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct
     5341 gttcagtcag gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcgggt
     5401 cctgtgcgcc aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg
     5461 cgacgttcat aaacagatcc atgccgatgc cgaagaagaa cgacaggcga ggagcgaagt
     5521 catcaatttt cagtccggca gagattgctg ctttgatgta ctcaatccca tcagcgagcg
     5581 taaatgctac ctgctgcacg cagttggcac ccgcttcacc catgtggtaa ccgctgatac
     5641 tgatggtatt aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc
     5701 gcattgacgg ttttggcggg taaatatagg tgttgcggca gaggtactct ttgaggatat
     5761 cgttctgaat ggtgccggtc agtttatcag gtgtaacacc ttgctcttcc gcggcgacga
     5821 tataaaacgc cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttat
     5881 ccagcgggat ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg
     5941 ctttgccgac gtcgcccgcc acgcgcgggt tatcggagtc gtagcctcgg tgggtggcaa
     6001 ggtcaaacgc aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt
     6061 tggactcttt tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat
     6121 acatagtggc acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct
     6181 ccagattatc gagatcggct tcggtataca gcggcttgat ggtgatccct tccgcggttt
     6241 gctgaaccag cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt
     6301 gccactcctg cacgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt
     6361 gcgcctacgg tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt
     6421 cgttttttgc cgcctgagat ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa
     6481 gtgaggacaa cgcctgatat gtgctatcag gcattatttg atggattaat cctgacgaag
     6541 gactttgtga ccataatcga gcaacgcatc agtgacttta cgcatcatgc ggctttcagg
     6601 agcaaagcgg tgccagtaga gcatccggcg ttgaaatagc cctggcgtta agtcaatcag
     6661 ttcaccgctg gctagttctt tctcgatttg caggtgcggg atcatacagc aggtggtgcc
     6721 ctggcgagca agttgcacga acgcttctga agaattaacg atatggcagg gcacgctgcc
     6781 tggaggcaga tcgaagtttt gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa
     6841 ggcgaccaca ggtgctttca ataatgccga acgcgttacg ccgttaggga aatatttttc
     6901 ggcaaacggt tttgagctga cgaacagata gtcaagcgca ccgagcttat cgacaaggca
     6961 actcggcagc gcctgatgtt gaatactcac cgcgccgacc acttcgccgc ggcgcagacg
     7021 ttcctgagta cgggtttcat cttctacctg caagttgagg cggataggcg aatcagccaa
     7081 tacaggagcc agtgccggaa gcaaccacgt cgccagactg tcggcgttga ccgccagtga
     7141 aagcagcagc ggagtcgaac cggtttgttc atcgcccagc cactcttctt ccagcaactc
     7201 cacctggcgc agcagtgcca gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt
     7261 acgcaccaac agcggctgcc cgaacatatt ttccagttgc ttaatgcgct gtgagacggc
     7321 tgattgtgta atgcacagct tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc
     7381 atccagtgcc tgtaatgttc tgtagtccgg gcgtttcatt gctgttgtct gctcctggta
     7441 aatgcttttt cctgcactat gacacaattt tgtgtcaggt tgcatatacc gcagccgtcg
     7501 gcgggtaaca tttgtatcaa tggttaaacg cgcagcttaa aagatcaggg atttgcgcta
     7561 ttgcatttcg cttagcgact tcgcgccctg gttatgttga atggcgtggc gttattgcct
     7621 ctgttcgcac cgtggagcgg catacccaga ccgttgtatg ctttatttgc ggggcgaaag
     7681 gggatgcccg ccattgcgcg atattttatg gatgagttaa ccacccggct tgccaacggg
     7741 gtctgaatcg ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat
     7801 catgacgcag gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc
     7861 cggcaccatt gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg
     7921 cacgatgaaa ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact
     7981 gaaaagcctc ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt
     8041 tgatggcgca gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct
     8101 gacccgtgaa aaaatcattg cttcggttgc ggaaaaattt atctgtattg ccgacgcctc
     8161 caagcaggtt gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg
     8221 tagtgcagtg gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt
     8281 ggtgaccgat aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat
     8341 agcgatggaa aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa
     8401 ccgtggcgcg gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg
     8461 atctgacggg ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg
     8521 tcacgttttt acctggctat tgtcgattgc tctaaataaa tcctctaaac cagcatattc
     8581 aaccaagaaa tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc
     8641 cgcaatatta ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa
     8701 gacaggattg ggtaaatggc aaaggtatcg ctggagaaag acaagattaa gtttctgctg
     8761 gtagaaggcg tgcaccaaaa ggcgctggaa agccttcgtg cagctggtta caccaacatc
     8821 gaatttcaca aaggcgcgct ggatgatgaa caattaaaag aatccatccg cgatgcccac
     8881 ttcatcggcc tgcgatcccg tacccatctg actgaagacg tgatcaacgc cgcagaaaag
     8941 ctggtcgcta ttggctgttt ctgtatagga acaaaccagg ttgatctgga tgcggcggca
     9001 aagcgcggga tcccggtatt taacgcaccg ttctcaaata cgcgctctgt tgcggagctg
     9061 gtgattggcg aactgctgct gctattgcgc ggcgtgccgg aagccaatgc taaagcgcac
     9121 cgtggcgtgt ggaacaaact ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt
     9181 atcatcggct acggtcatat tggtacgcaa ttgggcattc tggctgaatc gctgggaatg
     9241 tatgtttact tttatgatat tgaaaataaa ctgccgctgg gcaacgccac tcaggtacag
     9301 catctttctg acctgctgaa tatgagcgat gtggtgagtc tgcatgtacc agagaatccg
     9361 tccaccaaaa atatgatggg cgcgaaagaa atttcactaa tgaagcccgg ctcgctgctg
     9421 attaatgctt cgcgcggtac tgtggtggat attccggcgc tgtgtgatgc gctggcgagc
     9481 aaacatctgg cgggggcggc aatcgacgta ttcccgacgg aaccggcgac caatagcgat
     9541 ccatttacct ctccactgtg tgaattcgac aacgtacttc tgacgccaca cattggcggt
     9601 tcgactcagg aagcgcagga gaatatcggc ctggaagttg cgggtaaatt gatcaagtat
     9661 tctgacaatg gctcaacgct ctctgcggtg aacttcccgg aagtctcgct gccactgcat
     9721 ggtggtcgtc gtctgatgca catccacgaa aaccgtccgg gcgtgctaac tgcgctgaac
     9781 aaaatcttcg ccgagcaggg cgtcaacatc gccgcgcaat atctacaaac ttccgcccag
     9841 atgggttatg tagttattga tattgaagct gacgaagacg ttgccgaaaa agcgctgcag
     9901 gcaatgaaag ctattccggg taccattcgc gcccgtctgc tgtactaatt ccccttctct
     9961 gaaaattaac gggcaggtca ctgacttgcc cgttttttta tcccttctcc acaccgttgc
    10021 cgcgttgcgc attctacttt gcgagtctgc tccagaaaat aaatcaaagt cagatcaatg
    10081 cgttgtgttt atttaatggg gatttacgat ggcgggacag tatggggctg taacggtaaa
    10141 gccctcaccg aagcgagggc ttaaaggaga agggttatga tgcgacttgt catcatactg
    10201 attgtactgt tactgataag tttcggagct tattaacagt cagtctctgg ggaggagtca
    10261 tcctccctta cccttactca ctaaattagg tcaaagaatc aacgatgtca atcagggcga
    10321 tgcggggtgc atcgccctga accgaacttt ttgatagcca cttcatcgcc cacgtttact
    10381 gccgtaatag atcctttttc ccaaaaatta tctctgtttt gcatggggtt tttcgaacac
    10441 gctcgaagaa aagatttaaa ggtagatcaa tgcgttgtgg ttatttaaag agagcttacg
    10501 atagctgggc agcatggggc tgtaacggta aagccctcac cgaagcgagg gcttgaagga
    10561 gaagggttat gatgcgactt gccatcatac tgattgtact gttactcata agtttcccag
    10621 cttattaaca gtcagtcaca ggggaggagc aatcctccct tacccttact cactaaatta
    10681 ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg tatcgccctt accactccca
    10741 gactttcgac ggtgtaacca ccgcaggaag agggatatcc cactcttcaa cggggagttt
    10801 ttccaccaac tgacaatcat gcgcataacc caccggttgc gttttatagt gctgccagtt
    10861 ttgtaaggtc cgatcataaa aaccaccgcc cattcccagg cgctgaccgt actcatcaaa
    10921 ggcgaccagc ggtgtgatca gcacgtctaa tcgggaaagg ggtagcacgt cacgcacatc
    10981 cagttttagc tcatggattt tcaacctgtt catcaccagt tcgctttgcg gatggtaatt
    11041 caggaacagc aaattaccag cactaaaggg atgcaaaact ggaagatata cgcgcttacc
    11101 ggcgcgccag agttgttcta ttagcggctg ggtgtcgagt tcaccatcaa aagagaggaa
    11161 tacagcgacc gtatgcgcca tcaccaccgg gggataagtc atcatccggg tagcggcttg
    11221 ttgacccatt tcctgctgtt gttccggcgt taacgcacga cgacgttgcc gaatcatttt
    11281 gcggatttct tgtcgggata atgtcagtgg gagttctggt agttgcgtca tggctggtgc
    11341 cagataagaa gggaatctcc gagatgccgc cgcaggctgt aacccttgaa cccttggttc
    11401 aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg gaccgagcat gctcaccaac
    11461 cgcggagcgc cacattcttg tggtatgaaa tatcggctca ggggactggc ccgcttgcga
    11521 acatctcaga gaaattttgt cttcacggtt actctaccac agtaaaccga aaagtgtcat
    11581 tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc ttgttctatg
    11641 gtctgctgca gcatccgaat acgctgttcc atacttgccg cgtagtcacg agtcttcgct
    11701 ttttcttgcg ctaactcata gctgatattc aatgcggcaa tgaagaccaa ctgttcagta
    11761 tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc gtccgctgcc
    11821 tgattcaacg catccctttg gtcaggcggg cagttcacac gcagtgaacg gccaaaaatt
    11881 tggatatcga cgggttgtgc agacatgcca ccttcctgct gattgactgc gctgccttcg
    11941 tctttagacc catggtctgc gaaggggcga cactatagct accctgatga gaagagacaa
    12001 gcccttttct ggtccaccag ggcccaaagt ggtagcatat catgaatatt cctccctttg
    12061 acgacgaatg cttatgtcta tacagaacga aatgcctggt tacaacgaaa tgaaccagta
    12121 tctgaaccaa caagggacgg gtctgacccc tgccgagatg catggtttaa tcagtgggat
    12181 gatatgtggc ggtaacgatg acagctcatg gctgccgcta cttcacgacc tgacgaacga
    12241 aggcatggct ttcggtcatg agctggcaca ggcactgcgt aaaatgcact ctgccaccag
    12301 cgatgccctg caggatgacg gcttcctttt tcagctttat ctgcctgatg gcgatgatgt
    12361 cagcgttttc gatcgggctg atgcattggc aggttgggtc aatcacttcc tgcttggtct
    12421 tggcgttacg caaccgaagc tggataaagt gaccggcgaa accggtgaag ctatcgacga
    12481 tctgcgtaac attgcgcaac tgggttacga cgaagacgaa gatcaggaag agcttgaaat
    12541 gtcgcttgaa gagatcatcg aatacgttcg tgttgccgcg ctgttatgcc acgacacctt
    12601 tactcatccg caaccgaccg cgccagaagt acaaaaaccg actctacact aaaaacaaaa
    12661 aacgtaagga gagtgttatg agtgagatat cccggcaaga gtttcagcgt cgccgtcagg
    12721 ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca ccagaagtaa
    12781 cacgtagcgc cgacagcgaa tacccctatc gtcagaacag tgacttctgg tacttcaccg
    12841 gctttaacga accggaagcg gtgctggtgc tgattaaaag cgatgacact cataaccaca
    12901 gcgttctgtt taaccgcgtt cgcgacctga cggcggagat ctggtttggc cgtcgcttag
    12961 gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc actggcattc agcgaaatca
    13021 atcagcaact ttatcaacta cttaacggcc tggatgtggt ttaccatgcc cagggcgaat
    13081 atgcatatgc tgatgaaatc atgaacagtg cgctggaaaa actgcgtaaa ggttcgcggc
    13141 aaaatctcac cgcaccggca acgatgatcg actggcgtcc tgttgttcat gaaatgcgcc
    13201 tgttcaaatc gccagaagag attgccgtac tccgccgcgc gggagaaatc accgccatgg
    13261 cacatacacg ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccat ctggaaggcg
    13321 aaattcacca cgaatttaac cgccacggtg cgcgctatcc gtcctataac accattgtcg
    13381 gcagcggtga aaacggctgc attctgcact acaccgaaaa cgagagtgaa ctgcgcgacg
    13441 gtgacctggt gttgattgac gcgggttgtg aatacaaagg ttacgcgggc gatattaccc
    13501 gcaccttccc ggtcaacggc aaattcaccc aggcccagcg tgaaatctac gacattgtgc
    13561 tggagtctct cgaaaccagc ctgcgcctgt atcgtccggg aacttccatt ctggaagtca
    13621 ctggtgaagt ggtgcgcatc atggttagcg gcctggtaaa actcggcatt ctgaaaggtg
    13681 atgttgatga actgatcgct cagaatgccc atcgtccttt ctttatgcat ggccttagcc
    13741 actggttagg gctagatgtc catgacgttg gcgtttatgg tcaggatcgc tcgcgcattc
    13801 tggaaccggg catggtactg accgtagagc cagggctgta tattgcgccg gatgcagaag
    13861 tgccagaaca atatcgcggt atcggcattc gtattgaaga cgacattgtg attaccgaaa
    13921 ccggtaacga aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc gaagcgttga
    13981 tggctgctgc gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg cgggcgcgac
    14041 gctggcgctg gctatttccc ggttaagtca cggggcgctg ccggtacatt tgattgaagc
    14101 gactgcgcca gagtcacatg ctcatccggg ctttgatgga cgagcgatag cgctggcggc
    14161 gggtacctgt cagcaactgg cgcgcatcgg cgtctggcaa tctctggcgg attgcgcaac
    14221 tgccatcacc accgtgcatg tcagcgatcg tggtcacgct ggatttgtca ccctcgccgc
    14281 agaagattac caactggcgg cgctgggaca ggttgtcgaa ttgcacaatg tcgggcaacg
    14341 gctgtttgca ttgctgcgta aagcacctgg cgtaacgctg cattgccctg atcgcgtggc
    14401 taacgttgcc cgtactcaga gtcacgttga agtgacgctg gagagtggcg agacgctgac
    14461 gggccgcgtg ctggtagcag ctgatggcac ccattcagcg ttagccaccg cgtgcggcgt
    14521 tgactggcag caggagcctt acgaacaact ggccgtgatt gccaacgttg cgacttccgt
    14581 tgcgcacgaa ggccgcgctt ttgaacgctt cacgcaacat ggcccgctgg cgatgttgcc
    14641 gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc gcgaagaggt
    14701 gctgtcgtgg agcgacgaga agttttgccg tgaactccag tcggcctttg gctggcgact
    14761 tggaaaaatt acccacgccg gtaaacgcag tgcttatccg ctggcgttaa cccgcgccgc
    14821 cagagctatt acccatcgta ccgtgctggt gggcaatgcg gcgcaaactc tgcacccgat
    14881 tgccgggcaa gggtttaacc tcggtatgcg agatgtcatg agccttgcgg aaaccctgac
    14941 tcaggcgcat gagcgcggag aagacatcgg tgattacggc atattgtgcc gctatcagca
    15001 gcgtcgacag agcgatcgcg aagcaaccat cggcgtcacg gacagcctgg tacatctttt
    15061 tgccaaccgt tgggcaccgc tggttgtcgg gcgcaacatc ggactgatga cgatggaatt
    15121 attcaccccg gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg cgcgttgaag
    15181 gcggttttaa ggaataaaca atgcaatgtg ttgatgtagc cattgttggt ggcggcatgg
    15241 tggggctggc ggttgcctgc ggcttacagg ggagcggctt acgcgttgcc gtactggagc
    15301 agcgcgtacc ggaacctctg gcggcggatg caccaccaca actgcgcgtt tcggctatca
    15361 atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt ctctctcgta
    15421 gggccagttg ttatcacggt atggaagtgt gggacaaaga cagttttggt cacatttcgt
    15481 ttgacgatca aagcatgggc tatagccatc ttgggcatat cgttgaaaac tcagtgattc
    15541 actacgcgct gtggaacaaa gcgcagcagt cgtcagatat cactctgttg gcccccgcag
    15601 aattacagca ggtcgcctgg ggagaaaatg aaactttcct gacgctgaaa gatggcagca
    15661 tgttaacggc gcgtctggtg attggcgcgg acggtgctaa ttcctggttg cgcaacaaag
    15721 cagatatttc gctgactttc tgggattatc agcatcacgc gctggtagcg accattcgca
    15781 cggaagaacc gcatgatgcg gtggcgcggc aggttttcca tggcgaaggc attctggcct
    15841 ttttaccgct tagcgatccg catctttgct caattgtctg gtcactgtcg ccagaggaag
    15901 cgcagcggat gcagcaggca agtgaagatg aatttaatcg cgcgttaaat atcgcttttg
    15961 ataatcgcct gggcttatgc aaggttgaga gcgcgcgtca ggtgttccca ctgacggggc
    16021 gttatgcgcg ccagtttgcc gcgcaccgtc tggtgctagt gggcgacgcc gcgcatacca
    16081 ttcacccgct ggcggggcag ggagtaaatc tcggctttat ggatgctgca gagctgattg
    16141 ctgaactgaa acggttgcat cgtcagggga aagacatcgg gcagtacatt tacctgcgcc
    16201 ggtatgagcg tagccgcaag cacagtgcgg cgctgatgct ggctggtatg cagggattcc
    16261 gcgatctgtt ttccggtacc aatccggcga aaaaactgct gcgtgatatt ggtttgaaac
    16321 tggccgatac gcttcctggc gttaaaccac aacttatccg ccaggcaatg ggattaaacg
    16381 atttgcctga atggctgcgt taaaaatttc tcttctgctg tttatttgat acccatcaca
    16441 ctttcatctc ccggtttttt cgccgggaga ttttcctcat ttgaaataaa ctaatttcac
    16501 ctccgttttc gcattatatt ttctaatgtc attatttttt gatttagtgt tttttgacat
    16561 ttttttagct cttaatattg ccttattcaa attagatttc taatggcatc atctttatat
    16621 agaaactggt gtgttttttg gttttttatt ctgtcgcgat tttggcgttt tttaaccata
    16681 agctaatgtg atgatcaatt ttaccttatg gttaacagtc tgtttcggtg gtaagttcag
    16741 gcaaaagaga acgattgcgt tggggaccgg gagtggctcc gatgctgggt ttcgtggtga
    16801 taatttcacc atgaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    16861 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    16921 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    16981 cggaatgttt gatgtgtcac atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    17041 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    17101 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gacctcatcg tctactactt
    17161 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    17221 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    17281 gattgccgtg caagggccga atgcgcaggc aaaagctgcc acactgttta atgacgccca
    17341 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggcgggcg atctgtttat
    17401 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    17461 ggccgatttc tggcgtgcgc tggtggaagc gggtgttaag ccatgtggct tgggcgcgcg
    17521 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    17581 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccggcag atcgtgactt
    17641 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    17701 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    17761 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    17821 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    17881 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    17941 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    18001 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    18061 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    18121 gttagcgcgg gcgatgactg cgcggttgcc gaatcagtaa aagcggcgtc agacatttat
    18181 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc tccggaactg
    18241 gtgaacagcg aaccgtatgc aggtggctgg atctttaaaa tcaaagccag cgatgaaagc
    18301 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga cgagtaacgg
    18361 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    18421 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    18481 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    18541 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggcgca caatcgttaa acgcgctgac
    18601 cggccagatt gtaccgaaag atattcagct tgcgaccccg ccgcaggttg gcgcaccggc
    18661 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    18721 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    18781 gctggaaaat ccgggctggt ataccgcgta cactccgtat caacctgaag tctcccaggg
    18841 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    18901 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    18961 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccgatg tgcatccgca
    19021 aacgctggat gtggtccgta ctcgtgccga aacctttggt tttgaagtga ttgtcgatga
    19081 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    19141 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    19201 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    19261 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    19321 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    19381 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    19441 gcaacatatc cgccgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    19501 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    19561 ccgcattcac cgtttgaccg atatcctggc ggcaggcctg caacaaaaag gtctgaagct
    19621 gcgccatgcg cactatttcg acactctgtg tgtggaagtg gccgacaagg cgggcgtact
    19681 ggcgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg cggttgggat
    19741 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagcg tgctgctggg
    19801 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    19861 tattcagcct gcgatgctgc gcgacgacga aatcctcact catccggtgt ttaatcgcta
    19921 ccacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    19981 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    20041 gatcccaatc acctggccgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    20101 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    20161 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    20221 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    20281 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    20341 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    20401 gggcgataac ctctcctgta tcatggtgac ttatccctct acccacggcg tgtatgaaga
    20461 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    20521 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    20581 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    20641 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    20701 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    20761 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    20821 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    20881 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    20941 gaaagaagaa accagcatca gcgagctgga tattgccaag cgcctgatcg actacggttt
    21001 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    21061 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    21121 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    21181 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    21241 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    21301 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa tacctgtaat tcactgattc
    21361 gactattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    21421 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    21481 gcgcgaatat ttaccctcac cccggccctc tccctgaaag ggcgagggga aaagcgtgcc
    21541 aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg aattaatttt
    21601 tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc atcgggcggg
    21661 caactgcatt actgttggcg aaagaagagt atacggtggc ggttaattat caacaaaacc
    21721 tccatgcggc gcaggaagtg gtgaacttaa tcacgcaagc cggtggcaag gcattcgtgc
    21781 tccaggcgga tatcagcgac gaaaatcagg tcatcgcaat gtttacagca atcgatcagc
    21841 acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc cagtgcaccg
    21901 ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg acgggatatt
    21961 ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc agtggcggcg
    22021 ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa taggttgatt
    22081 acgcggcatc gaaaggggcg attgatacgt taaccaccgg actatcgctg gaagtcgccg
    22141 cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa atgcacgcca
    22201 gcggcggcga gcttggacgc gtcgatcgcg ttaagtcgaa catccccatg cagcgtggtg
    22261 gacaggcaga agaggtcgcg caggccattg tctggctact aagtgataaa gcctcttacg
    22321 tcacgggaag ttttatcaat ttggcgggcg ggaaataaaa cagggaagtt gtctgaccgg
    22381 atgcaacaag tattgcatcc ggtacttcat cgacttaaag cttctcgccg ttgctggcaa
    22441 tcacctcttt gtaccagtta aagctcttct tacgtgaacg cgacatatcg ccagtaccgt
    22501 cgtcatgttt attcacatag ataaagccgt agcgtttgct gtactgcccg gtagtgaatg
    22561 acacgcagtc gatgcagccc cacggtgtgt agcccatcag atccacacca tcgtaagtca
    22621 ctgctttttt catctcttca atatgggcgc gcaggtagtc aatgcggtag tcgtcgttga
    22681 tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc gttttcgaca ataaacagcg
    22741 gcctctgata acgctcatac agttcgcaaa gggcatagcg cagacctact ggatcaatct
    22801 gccagcccca gtcagacgct ttaacatacg ggttcggtac gctgccttca aaaccagaga
    22861 tcgcatcgcc ggtgccgcct tcggccttca ctgcgttgat catgtaatag ctgaaaccaa
    22921 gataatcgca ggtgccttca cgcagtacat ccagatcgcc atcttccatt ttgatgttaa
    22981 atccgcgacg ctcccactcg ttcaacacat aggacgggta atagccgcgt agctgcacat
    23041 cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa catcacatcg tccgggttac
    23101 aggagtaagg atagagcggc accatcgcca gcatacagcc gactttcatc tccgggttaa
    23161 tgcgacgcgc ggctttcact gctagggcgc tggcgacaaa ctggtgatgc agcacctggt
    23221 acatcgtctc ttccgggttg tcatgctcgg tatacaccac gccggagcag cagtaaccaa
    23281 acagcggcgc acgccagtta cgctggttgt ttatttcgtt gaaggtcatc cagtatttga
    23341 ctttatgctt gtagcgttcg aataccactt ccgcgaaacg gacaaagaaa tcaacgactt
    23401 tacggttggt ccagctaccg tattgctgca ccagatgcag cggtatttca aagtgggaga
    23461 gggttatcac cggttcgatg ttgtatttca gcagttcatc gaacagagag tcgtaaaact
    23521 tcagcccttc ttcgtttggc tgagcttcat caccttttgg aaaaatacgc gtccaggcga
    23581 tggaagtccg aaaacatttg aagcccattt cggcaaatag cttgatgtct tccttgtagt
    23641 gaccataaaa atcaacggct tcatggtttg gatagtattt tcctggcaag acttctttgg
    23701 tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac gtcacaaatg ctcggtcctt
    23761 tgccgccttt gttccagccg ccttcgacct gatgagcggc aacggcgcca ccccataaga
    23821 aatctttcgg taaggtgagt tttttcacta tcataggctc cagatgagtc aattcttaca
    23881 ggaaatagta ccatgcagcc aaacgacatc actttttctc aacgtttcca ggatgacatt
    23941 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    24001 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    24061 gcaacctcaa ccgtaacgct ggatacgctg acggaaaaac atgcagagca ggaaaatatg
    24121 accctgactg aactgataaa ggtcattgcc gacatctatc ccggtcagac acagttttat
    24181 gtgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttctcattt
    24241 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    24301 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctccgga
    24361 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    24421 caacagcgtc agtcacggca ttgggttggt gtttggtatc gttgggctgg tgttgctact
    24481 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagctaca gcctctatgg
    24541 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    24601 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    24661 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    24721 tatctggagc ctggcattgc tggggattct gtttaaactg accatcgcgc accgattcaa
    24781 aattttatct ctggtgacct atctggcgat gggctggctg tcgctggtgg taatttatga
    24841 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    24901 ttcgctcgga gtgattttct atgtctgcaa acgcattccc tacaatcatg ccatctggca
    24961 cggcttcgtg ctcggcggca gtatgtgcca ctttctggcg atctatttgt atattgggca
    25021 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg
    25081 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    25141 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    25201 atgcctgatg cgatacagag atttactctt cgagtgaata cggcagcggc tcgatatgca
    25261 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    25321 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    25381 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    25441 cgctacctgc cagcaaccag agcgcacgtt tattggcacc acggaatttt gctcgcgcca
    25501 ccatctcttg tccggtataa cagcctttct taaaactgat accgcccagc gcctggagat
    25561 tggtcgcctg tgggataaac tgcccgctgt tcgcggcatc aatcaccggg aaacccgctt
    25621 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    25681 cggtcagcat attggcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    25741 caaaccatag caaagtggtc gcgccttctt taactacctg tttttctctc gaaggtagtt
    25801 cactaaagag attggcgagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    25861 gctcgtcgtc tgccgcgatg gtcactttag agaacaccgc atattttttc agttcagtca
    25921 gctgcggttc acgcacgctg cgccgttcaa tccacgcaaa gccatcgccg tcgcggaaca
    25981 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    26041 tttctgtcat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    26101 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    26161 cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    26221 cttaacggcg tattaataac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    26281 tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa tgaagctctt gaattgttgt
    26341 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    26401 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    26461 aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    26521 ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    26581 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    26641 gatgcagaac tggaaatgat ggtccgactc atccagacac ggaaccggga acgtggtcct
    26701 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    26761 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    26821 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggcgta ttaatgctcg
    26881 ccagggggaa attcgattgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    26941 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    27001 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagctg aatggcggga
    27061 tttacggcgg attttgttgc aacaagagac gcaaagataa atatgacggc aaagcatatg
    27121 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    27181 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    27241 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    27301 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    27361 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaacaa
    27421 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    27481 tttcgccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    27541 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    27601 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    27661 taacgtaagc ttctcgattc tgctgtggct tttgcggcta gtgtatcaga atcgctttag
    27721 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    27781 ggatctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    27841 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    27901 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    27961 agagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    28021 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    28081 gaaattgcca cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    28141 ggcaccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    28201 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctacg
    28261 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttaggtga
    28321 agaggccgtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgttgaatgg
    28381 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    28441 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    28501 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    28561 ggtgcagatg ctactgggcc acagcgatct ctccaccacg caaatttata cgcatgtcgc
    28621 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    28681 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    28741 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcattaaaag
    28801 cagcgatatt cagccagcac ccgtggctgg catgaagact gtgctgacta acagcggtgt
    28861 gttgtacatc accgatgacg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    28921 cgcggctccg gtcaatgtca ccaataaaat gctgctaaag cagttgaatg cgcttgaaaa
    28981 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    29041 tacctgtggg tactgccaca aactgcatga gcaaatggca gactacaacg cgctggggat
    29101 caccgtgcgt tatcttgctt tcccgcgcca ggggctggag agccaggctg agcaacaaat
    29161 gaaagccatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggctggtaa
    29221 agctgccact ccggcaagtt gcgatatcga tattgccgat cattacgcac ttggcgtcca
    29281 gcttggcgtt agcggcactc cggcaattgt gctgagcaat ggcacacttg ttccgggtta
    29341 ccagccgccg aaagagatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    29401 ttaattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    29461 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    29521 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    29581 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    29641 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    29701 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    29761 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    29821 aacggtattt cctcccatgc gggggttgaa cacgctcgct cgttgggcat tccggttatt
    29881 gttaccgatc accatttgcc aggcgaaaca ttacccgcgg cggaagcgat cattaaccct
    29941 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    30001 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    30061 gcaattccta acctggcaga actgctggat ctggtcgcgc tggggacagt ggcggacgtc
    30121 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    30181 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaaccgtga tgcacaaaaa
    30241 ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc tcaatgctgc cgggcgactg
    30301 gacgatatgt ccgtcggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    30361 ctggcaaatg aactcgatgc gctaaatcag acgcgaaaag agatcgaaca aggaatgcag
    30421 attgaagcct tgaccctgtg cgagaaactg gagcgaagtc gcgacacgct acccggcggg
    30481 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgcatc
    30541 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgatgg tacgctgaaa
    30601 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    30661 ctctaccccg gtatgatgct gaagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    30721 gaagaggata aattcgaact ctttcaacaa cggtttggcg agctggttac cgagtggctg
    30781 gacccttcgc tattgcaagg cgaagtggtg tcagacggcc cgttaagccc ggccgaaatg
    30841 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    30901 ccgctgtttg atggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    30961 aaagtcatgg tcgaaccggt cggcggcggt ccgctgctgg atggtattgc ttttaatgtc
    31021 gataccgccc tctggccgga taacggcgtg cgcgaagtgc aactggctta caagctcgat
    31081 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    31141 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    31201 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    31261 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    31321 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    31381 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    31441 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    31501 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    31561 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgcta
    31621 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    31681 gcgtatgtat ttgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    31741 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    31801 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaagagcc cgtttgactc
    31861 cggcggtcgt cgccacacgt cgttcagctc cgcgtttgtt tatccggaag ttgatgatga
    31921 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    31981 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    32041 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aataaagacc aggccatgaa
    32101 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgctg agaaacaggc
    32161 gatggaagat aacaaatccg acatcggctg gggcagccag attcgttctt atgtccttga
    32221 tgactcccgc attaaagatc tgcgcaccgg ggttgaaacc cgcaatacgc aggccgtgct
    32281 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    32341 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    32401 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    32461 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    32521 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    32581 gggtaaagcc tcttttgtta ccctgcagga cgttggcggt cgtattcagc tgtacgttgc
    32641 ccgtgacgat cttccggaag gcgtttacaa cgagcagttc aaaaaatggg atctcggcga
    32701 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    32761 caccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggacaaat tccacggctt
    32821 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    32881 ccgcaacacc tttaaagtgc gttcgcagat cctctctggc attcgccagt tcatggtaaa
    32941 tcgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    33001 gcgtccgttt atcacccatc ataacgcgct ggatcttgac atgtacctgc gtatcgcgcc
    33061 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    33121 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    33181 ctatatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    33241 tctggcacag gatattctcg gtaagacgga agtcacctac ggcgacgtga cgctggactt
    33301 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    33361 cgacatggcg gatctggaca acttcgactc tgcaaaagcg attgctgaat ctatcggcat
    33421 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    33481 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    33541 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    33601 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcagcgctt
    33661 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgacgaaga
    33721 ttacgtcacc gcactggaac acggcttacc gccgacagca ggtctgggaa ttggtatcga
    33781 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    33841 gatgcgtcca gtaaaataag cattacgtta tgctaataac cccggcaaat gtcggggttt
    33901 tttaattgtg ctgcgcgaaa gcagacaaca attttcttgc ttcactattg gctgcctgca
    33961 ttaccatcca cggactgaac gcccacggcg tggcatcaat accgtgtaat acatctgcta
    34021 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctgttggtgc
    34081 gtgcggcaaa taccggacac acttcatttt ccacaatgcc attcggatcg gtggcgcgat
    34141 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    34201 aacggcggat cactgcgtct tcgttgcttt ctcccagttg tgggtgccca caaaccgagt
    34261 tagtccacac gccaggccat gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    34321 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    34381 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    34441 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    34501 tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    34561 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    34621 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacagcagac tgaatatagc
    34681 gcgcattaaa agatttatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    34741 gccacatacg tactgtcctg acttttaaat ctttatcaga gacggattcc ttcaccaggt
    34801 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    34861 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    34921 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    34981 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    35041 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    35101 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    35161 tatgaacgcg cgtcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    35221 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg cgaataatat
    35281 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    35341 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgataatcg
    35401 gcataccaaa cgacatcggg gtgacaatcg caaaccacaa ggcatcatgt agcccggata
    35461 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    35521 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    35581 caaaggagat acctgaataa acgggattac catattgcgg attcccttta cctccggcgg
    35641 cccagtcaat acccacctga atgatgctaa gtccgacaga agtaataacc acaccggtaa
    35701 ccagtggcgg gaataaaggc atcaagcgac cgataagtgg cgctaataat gtggtgataa
    35761 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc aatatccggg ttcatcccaa
    35821 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    35881 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    35941 ccgagctaat gagcatcgca atagcttctt ttgagaggcc cagtcggtca ccaatcataa
    36001 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    36061 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    36121 agagaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagagtaaaa
    36181 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    36241 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg gttaaaccac
    36301 gaaaatgaga agagaacctg acaatggaat tatctgtaat tttctcatat tgatatttca
    36361 ttaatcattc tggcatgtcg tactctcgca tttaatcgtt tttatctgga tagcactctt
    36421 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    36481 agtcgcaaaa ttataattaa aacggtcatg ctacctaata
//
LOCUS       DAJTNN010000019        35563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTNN010000019 DAJTNN010000000
VERSION     DAJTNN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.019"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            98..445
                     /gene="grxD"
                     /locus_tag="OO150_000855"
     CDS             98..445
                     /gene="grxD"
                     /locus_tag="OO150_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5515089.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(496..4903)
                     /locus_tag="OO150_000856"
                     /pseudo
     CDS             complement(496..4903)
                     /locus_tag="OO150_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4996..5643)
                     /gene="rnt"
                     /locus_tag="OO150_000857"
     CDS             complement(4996..5643)
                     /gene="rnt"
                     /locus_tag="OO150_000857"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5515090.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRSAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMIAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5746..6153)
                     /gene="gloA"
                     /locus_tag="OO150_000858"
     CDS             complement(5746..6153)
                     /gene="gloA"
                     /locus_tag="OO150_000858"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5515091.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6234..7331)
                     /gene="nemA"
                     /locus_tag="OO150_000859"
                     /pseudo
     CDS             complement(6234..7331)
                     /gene="nemA"
                     /locus_tag="OO150_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            complement(7368..7967)
                     /gene="nemR"
                     /locus_tag="OO150_000860"
     CDS             complement(7368..7967)
                     /gene="nemR"
                     /locus_tag="OO150_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5515092.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYTAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8070..8309
                     /locus_tag="OO150_000861"
     CDS             8070..8309
                     /locus_tag="OO150_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5515093.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8358..9254
                     /locus_tag="OO150_000862"
     CDS             8358..9254
                     /locus_tag="OO150_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5515094.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9335..9856
                     /gene="sodC"
                     /locus_tag="OO150_000863"
     CDS             9335..9856
                     /gene="sodC"
                     /locus_tag="OO150_000863"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5515095.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9857..11868)
                     /locus_tag="OO150_000864"
                     /pseudo
     CDS             complement(9857..11868)
                     /locus_tag="OO150_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994342.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11868..12725)
                     /locus_tag="OO150_000865"
     CDS             complement(11868..12725)
                     /locus_tag="OO150_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5515096.1"
                     /translation="MSIKTIKYFSIIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPMSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12791..12964)
                     /locus_tag="OO150_000866"
     CDS             complement(12791..12964)
                     /locus_tag="OO150_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5515097.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            13165..13599
                     /gene="slyA"
                     /locus_tag="OO150_000867"
     CDS             13165..13599
                     /gene="slyA"
                     /locus_tag="OO150_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5515098.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13646..14113)
                     /gene="slyB"
                     /locus_tag="OO150_000868"
     CDS             complement(13646..14113)
                     /gene="slyB"
                     /locus_tag="OO150_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5515099.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14387..15496
                     /gene="anmK"
                     /locus_tag="OO150_000869"
     CDS             14387..15496
                     /gene="anmK"
                     /locus_tag="OO150_000869"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5515100.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VIRPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            15594..15923
                     /gene="mliC"
                     /locus_tag="OO150_000870"
     CDS             15594..15923
                     /gene="mliC"
                     /locus_tag="OO150_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5515101.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15982..16638
                     /gene="pdxH"
                     /locus_tag="OO150_000871"
     CDS             15982..16638
                     /gene="pdxH"
                     /locus_tag="OO150_000871"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5515102.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16767..18041
                     /gene="tyrS"
                     /locus_tag="OO150_000872"
     CDS             16767..18041
                     /gene="tyrS"
                     /locus_tag="OO150_000872"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5515103.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18100..18963
                     /gene="pdxY"
                     /locus_tag="OO150_000873"
     CDS             18100..18963
                     /gene="pdxY"
                     /locus_tag="OO150_000873"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5515104.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(19007..19612)
                     /gene="gstA"
                     /locus_tag="OO150_000874"
     CDS             complement(19007..19612)
                     /gene="gstA"
                     /locus_tag="OO150_000874"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5515105.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNETLKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19718..21220)
                     /gene="dtpA"
                     /locus_tag="OO150_000875"
     CDS             complement(19718..21220)
                     /gene="dtpA"
                     /locus_tag="OO150_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5515106.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21831..22466)
                     /gene="nth"
                     /locus_tag="OO150_000876"
     CDS             complement(21831..22466)
                     /gene="nth"
                     /locus_tag="OO150_000876"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5515107.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22466..23161)
                     /gene="rsxE"
                     /locus_tag="OO150_000877"
     CDS             complement(22466..23161)
                     /gene="rsxE"
                     /locus_tag="OO150_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5515108.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(23165..23785)
                     /gene="rsxG"
                     /locus_tag="OO150_000878"
     CDS             complement(23165..23785)
                     /gene="rsxG"
                     /locus_tag="OO150_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5515109.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23789..24847)
                     /gene="rsxD"
                     /locus_tag="OO150_000879"
     CDS             complement(23789..24847)
                     /gene="rsxD"
                     /locus_tag="OO150_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5515110.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24848..27070)
                     /gene="rsxC"
                     /locus_tag="OO150_000880"
     CDS             complement(24848..27070)
                     /gene="rsxC"
                     /locus_tag="OO150_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5515111.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(27063..27641)
                     /gene="rsxB"
                     /locus_tag="OO150_000881"
     CDS             complement(27063..27641)
                     /gene="rsxB"
                     /locus_tag="OO150_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5515112.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(27641..28222)
                     /gene="rsxA"
                     /locus_tag="OO150_000882"
     CDS             complement(27641..28222)
                     /gene="rsxA"
                     /locus_tag="OO150_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5515113.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFTAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(28299..28739)
                     /locus_tag="OO150_000883"
     CDS             complement(28299..28739)
                     /locus_tag="OO150_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5515114.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            complement(28825..29040)
                     /gene="ydgT"
                     /locus_tag="OO150_000884"
     CDS             complement(28825..29040)
                     /gene="ydgT"
                     /locus_tag="OO150_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5515115.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(29313..29438)
                     /gene="blr"
                     /locus_tag="OO150_000885"
     CDS             complement(29313..29438)
                     /gene="blr"
                     /locus_tag="OO150_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5515116.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            29651..30691
                     /locus_tag="OO150_000886"
     CDS             29651..30691
                     /locus_tag="OO150_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5515117.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(30726..31727)
                     /gene="add"
                     /locus_tag="OO150_000887"
     CDS             complement(30726..31727)
                     /gene="add"
                     /locus_tag="OO150_000887"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5515118.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31830..33002)
                     /gene="malY"
                     /locus_tag="OO150_000888"
     CDS             complement(31830..33002)
                     /gene="malY"
                     /locus_tag="OO150_000888"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5515119.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(33012..34604)
                     /gene="malX"
                     /locus_tag="OO150_000889"
     CDS             complement(33012..34604)
                     /gene="malX"
                     /locus_tag="OO150_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5515120.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQTQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            34779..>35563
                     /gene="malI"
                     /locus_tag="OO150_000890"
     CDS             34779..>35563
                     /gene="malI"
                     /locus_tag="OO150_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5515121.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFG"
BASE COUNT         8902 a         9141 c         9262 g         8258 t
ORIGIN      
        1 aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg cactgacccg ctacaatgtc
       61 agattactgc cgtaaagaag tttgaggaag caagacaatg agcaccacta tcgaaaaaat
      121 ccaacgccag attgctgaaa acccgatcct gctgtacatg aaaggttcac cgaaactgcc
      181 gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc gcatgtggcg aacgttttgc
      241 ctatgttgat attctgcaga atccggacat tcgtgcggaa ctgccgaaat atgctaactg
      301 gccgaccttc ccgcaactgt gggttgacgg cgagctggtc ggcggttgtg atatcgtgat
      361 cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa gaaactgccg ctaaatacaa
      421 gtctgaagag ccggacgcgg aataatccgt cctgcaatac tgacggatgc gaacgggcat
      481 ccgtcagtcc tttctctatc cccaatccag cccttgtggc gaacttgaaa atcccgcctc
      541 acgcagcagc gtaaacatcg gcgtttgccg gaccggtaga tcattcactt ctgttagcgt
      601 aaagcgcagc cgtggttcgc gacgcagtgc ggtagtcagc gcgtggaaaa cctctggggc
      661 ttttcctgcc agaccagcat ttttttgcca ccttgcgcca gatagagtaa caatttgccg
      721 ctagaaacga ccaccagcgc acccgcccga cgcgttggaa ccagcgttgc cggatgtgca
      781 ggccagggca gaagatttcc ccacacatta gccggatcgt tggcagagag cgccactggt
      841 gtatagtggc gcgtttgcgt ggcttgtgtc gccagatcgc gccatcggtc aatagtcaga
      901 cgttcagcga attgcgcgcc acccagacct tctacaaaac gacctcgcat aattcgcccg
      961 gagtcttcca tacttcgaca aagcgtttgc atcgatggaa acccgccagg gatattttcg
     1021 gctatcaccg cctgacgact gatgatgccg tagcggtcga gcatattttc cgccagcgcc
     1081 agcatccttt cggtatcgtt tagtggctcc acctgcaata acgaccagcg tccagccaga
     1141 tttggtgtgt tgtaagatac cagcggcgag acgggttgcg catagacagg acgtccacga
     1201 cgagcccggt gactgcggcg agttgaggtg cgtgcgttgg aactgctgcg ggtgagggcg
     1261 cgtaacggtg cccaaatgtc gctggtgatg acgccttgcc agaccagcgc ccataacgcc
     1321 tcttgcaggg cagagagatc aaccgattcg ccgattttgt cgcgtatccg ttggctgatt
     1381 tgttgtgcaa accaggctcc tccgtcagcc agaacagcga ctatcgcttg ttgcagcgcc
     1441 gaacgattcg cctgatccgc ttcggcagga gtgaacgatt ctgcagcata ttcctgtaga
     1501 tgcagtgcca ccaggccgtc atcttcaccc agtttttttt gccccgacca gataaccgca
     1561 ccggttgcca gtaattcatc gagcatttct gacgaatagt cgcgtacgcg ggcaggcagg
     1621 atctggcttt cccagagtga ggcgggtaaa ccgactccgg caagctgttc gatcacccgc
     1681 atcacgccat ctacgccctc ataaacgcct ggcgatgttg aggcaaagag cgccgggcta
     1741 ccatcggtgg cgggtaatac gccctgacgt tccagcagca atcgcgcata ggtcgtggct
     1801 gcaacgggac gcgtcgcttc tctggcggct tgcagcgagc gcaaacgcag acgacgaaat
     1861 acttcatcgc tcacccagat gtcttgttgc agattcatca ccagaccctg ttcacgcagt
     1921 tgctgaagct gctcttcgac aatggcaata ccgagactaa actcatgagc cagttgttca
     1981 gccgtgacca gagcatgagc ccggagatag cgcagaaaga ggtcgcgaag cgggtaactg
     2041 actctatgta aataaatctc tggcaatgac tctggtagtc gtacgccgag ggcatcacgc
     2101 agcctggcgg catcatccat acaggcaaga cgctcctgtc cgctaatcat cgctgggaag
     2161 attcgtttta ctgcaagaag attttccaga tacgacgcaa cctcttcact gctgcctgta
     2221 tgccgttgcg ccaggtcttc aacggtcatt ggccccagtt cgcgcagcag gtcgaacaat
     2281 ccttcttcac ctttcgctct tctgccagga gccagtcgtt gcaactcttc ttccacctgg
     2341 cgaatgacct gcgggtcgag taattccccc ggatcgacct gtccgagtag attgcgcagt
     2401 aactcgctgt ccagcgacag tacggatgcc cggcgctctg ccagcggggc gtcgctctgg
     2461 tacataaatt ccgcgacata gccgaacaat aaacttgtgg cgaaaggcga gggcgtagtg
     2521 gtcgttacat cggatatttg aatttcgcca ccgttcaggc gacgcatcaa acgttccagt
     2581 gcgggaagat cataaacatc ttgcagacat tcgcgtagga tttcgagaat gaccggaaaa
     2641 tctggatatc cctgagcgat ttccagcaac tgactggcgc gcagccgttg ttgccaaagc
     2701 ggggtgcgat ggtccggagt gcgccccggc attaataatg cccgcgcggc gcattcgcga
     2761 aaacgggcgg cgaaaagtgc cgagctgcct accgcctcgc ggacaatttg cagcaacttt
     2821 tctggttcaa acaaaaaaat cgcggcgtcg ggcaatttac catcagtgtc aggaatgcgc
     2881 gcaacaatgc cgtcatcact ggcgaccaca gacgcgtcag cgcccagggt tcatgcaccc
     2941 gtcttccata gggagagtgc aaaataatac gccagtcgcc aatttcatca cggcaccgtt
     3001 cgaggaccag atggcgactc cccggaacga tgcccgtcgc gttgcgttgc tcgtcaatca
     3061 acccctgaat attggcgttc gtattttctt ctgccagcca cgggggaata gtcccggaaa
     3121 agaacgcgcc atccgccagt aaatgaagaa aatcgccgat catctcgcct aattcagccg
     3181 gacgtccgtt accttcacca cgccagaagg ggagccgggc agaacgaccc ggagcaggag
     3241 tcacaatcac ctgatcgcgg gtgatttgct ggatccgcca tgaggtagcg ccgagagtga
     3301 taatgtcgtt cacccgcgac tcatatacca tctcctcatc cagtttaccc acccgccgcg
     3361 aaccggcctt ttcttcacct tcgggtaata acacgctata catgccacga tccggaatgg
     3421 tgccgccgct ggtaaccgcc aacaattgag cgccaggtcg gacggtcaat atcccggtct
     3481 ccctgttcca gaccagtttg gggcgaaaag cagaaaaatc gccagaggga tagcgcccgg
     3541 aaagcatatc cagcgtggcg tcaaaaacac gtcttggcag atctttccac ggtgcggcac
     3601 ggcgtacgcg ggagtaccat tcgtctacct gtaatgcctc catcgccgcg gcggcaacgg
     3661 tttgctgcgc aaggacgtcg agtggattat gcggcggtgt caggttttcc agcctgcctg
     3721 cgaacataca ctctacaatg actgcggaat cgactaagtc acgccgggta cgagggaaaa
     3781 acagcccttt agatacaccg ccaacctgat gtccggcgcg accaatgcgt tgtaacccac
     3841 tggcaacaga aagcggcgtt gccacctgaa tcaccagatc cactgcgccc atatcaatcc
     3901 ccagttcgag actggaggtt gcgaccacgc agcgtaactc acccgatttc agcgcctgct
     3961 cggtgattgc tcgttgttct ttagagacgg agccgtggtg tgagcgggca ataaaaacgt
     4021 cgctactttg tacacggtta gaggttgcgc cggaggtcga ctcgaaatgg gccgcatcaa
     4081 cggcgataga cggggaacgc tgtaagcgtg cggcgtaaag ctcatttaat cgtgccgtca
     4141 gtttttccgc cagcccacgt gaattagtaa agacaatggt cgagcgatgg cgcaacactt
     4201 catcaaggat acccgtttca atatatggcc agatggagcc ttcccggccg gcatggctgt
     4261 cttcgccggt gctgctggcg accgatgaga catcatccat attggcgacc ggtacgacaa
     4321 ttcgtatctg cggatggcgc attgcgggcg ggttgactac cgtaaccggg cgatcgccac
     4381 caagaaaggc tgccacatcg ctggctgagc gcacagtggc agaaaggcca attcgctgtg
     4441 ctgaggtgtg gagcagcgca tcgagccgct ccagacttaa cgccagatgc gcaccacgtt
     4501 tactgcccgc taccgcgtgg acttcatcaa taattaccgt ttcgccgcgc cgccgccgtt
     4561 catcggcaat acccttcaac gggagctgca agttgcgctg aacgtcggtg cccagagctt
     4621 ttatcggtga aatatagagg atgcgtgagg ttttacgctt atgcgcctcg cgggtatctt
     4681 cgccgccttc gcggaagagc cgatcgaggg cgtagagaaa tgctgccagc gttttcccgg
     4741 agccggtcgg tgcaatcacc agcgcatgtt cgcttcgcgc cgccacatgc caggtttgcg
     4801 actggacagc ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc gccggtgaaa
     4861 acacgtccgg caggagcgat gaagggtctg gattatctgc cattaacgct attccccgtg
     4921 aatatcatgc aggatgcgtg gaatagcctc actatagatg cctgaatcat tgcatgtcat
     4981 caggcatcga ctcgattaca cctcttcggc ggcaggtagc ggccagcctc ccagacgttt
     5041 ccagcggttg acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt acagcgcgga
     5101 gtgcgcctgg gtgctgtcga agtccatgcc agcggtctgg caagcctttg acaatacggt
     5161 ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag ggtggaacgg
     5221 gttacgtttc agtgaggcgc gttctgcggc ggctatcata aagctgtgat caaaattggc
     5281 attgtgcgcc accataatgg cgcggttaca gccgctcgct ttaatacctt tacgtacaac
     5341 tttaaaaatt tcgtgcagcg cctcgtattc gctgaccgcg ctacgatcgg gatcgttcgg
     5401 gtcaatgccg ttgaaggcga gggcttctgg ttgcaaattt gcgccgacaa atggttcgac
     5461 gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt tcatccattt tcagggtgat
     5521 ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat ccggctgttt caacatcgat
     5581 cacaacagga taaaaaccac gaaaacggtc gcacagaccg gtaagttgag cgttatcgga
     5641 catctgattc tcttaatacg gataaattgc agcgcgcatt atgacaaata ttacttgtcg
     5701 atgcagtaaa gatgcgggcg cgatgagttc acgcccggca ggagattagt tgcccagacc
     5761 gcgaccggcg tctttctctt cgattaactc aattttgtaa ccgtccggat cttccataaa
     5821 cgcgataacc gtagtaccgc cttttaccgg acccgcttca cgggtcacgt tacccccgtt
     5881 ttgacggatt ttttcgcacg cttcagcggc gttatctacg ctaagcgcga tgtgaccata
     5941 agcagtgccg agttcgtatt tatccacgcc ccagttgtag gtcagttcaa tcaccgcttc
     6001 ttcggtttcc gggccgtagc caacaaacgc cagtgagtat ttgtattccg ggttttcgct
     6061 ggtacgcagc agtttcatgc ccagcacttt ggtataaaaa tcgatggagc gttgcaaatc
     6121 gccaacgcgc agcatggtat gaagaagacg catcttttta tcctcaaaat ggctaacaga
     6181 ttcaaaatgt tgttttagta tagcggcggc tttacgccgc tcgcaatgtt ggattacaac
     6241 gtcgggtaat cggtatagcc ttccgcgccg ccaccgtaga aactttcggc acgctgtggg
     6301 ttaagctcag ctttgcgctg caagcgggcg accagatccg ggttcgcaat ccagtcacga
     6361 ccaaatgcca ccgcatcaat taaccctttg ccgatcagcg tttcagcttt ttctactgtg
     6421 tatgcacctg cgccgataat cggaccgtgg aaacgggcgc gtactttttc gcggaacgca
     6481 tcagtatacg gttcaccccc cgcccaatct ggttctgaca tatgcagata agcaatgccg
     6541 cgtttaccca gttgttcaat cagatacagt gcatcggctt cttcattcgg gccgttatcc
     6601 gtgttctgga aagtaccgat tggcgaaatg cgaatgccaa tgcgatcggc accccattct
     6661 tcaatcccgg catcgaccac ttccagtacc agacgtgcgc gattttccac gctgccgccg
     6721 tactgatcgg tacgatggtt tgaagaagga gaaaggaact gatgcagcaa ataaccgtga
     6781 gcagagagga gctctaccag atcaaaaccg gcttcacgcg cgttagcaat ggcctgacgg
     6841 aaatcattga cgatacctgg aatctcttct agttcaagcg cacgcggcat ggatgtttca
     6901 acacggatcg cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc gctaagtgct
     6961 gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat gcgtccggtg
     7021 tgccacagct acacggccat atgaccattt tcagcatgaa cgccagcggt gatttttttc
     7081 catgcggcaa tttgctccgg actatggatg ccaggcgcac ctgcatatcc ttttacctgg
     7141 gcagaaattt gcgtggcttc actaataatc aaaccggcac tggcacgttg gcgatagtat
     7201 tccgccatca acggggtagg aatgtcaccc ggttcaatac tgcgcagacg cgtcagcggt
     7261 gccataaaaa tacggtttgc cgccgtgatc gcgcccactt tcagtgggga atacagtttt
     7321 tcagatgaca taaaggctcc cggtcgtctg gtgataaaaa atgcctgcta aacggcaggc
     7381 gtcgcaataa tgttttttac atgggccagc gcgttttcca gtggctcgaa actgcgcgaa
     7441 attttggcct gcagattcgc acccagccat agtgcgtaaa gcacttgtgc ctgttgtagc
     7501 ggttcgccac aaaaggttaa acaatggttc tcacggccat tttccagcgc ctgagagagc
     7561 agggcgatca cgccgcgagc acctttatcc atcgcgctgc gcatatcttc tgacagatcg
     7621 cacacttcgg cagagagttt tactgtcagg caaccactga tggttccatg ttggcaaaac
     7681 tggttcagtg tttgctggta ataagccagt atgcggtcgc ggtagttacc ttcgccggat
     7741 tgcagcaact cagtcagtcg ctggtgatat gcggtgtaat gacgctcaag catggcaacg
     7801 ccaaacgctt ctttagagcg aaagtagtga tagaaggacc ctttcggcac ttcagcggtt
     7861 tttagtaatt cgcttagccc catcccggtg aatccacgtt gcaggcaaag ttgctcgccc
     7921 gtcgccagga gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg ttggagtgta
     7981 gtagaccagt cggtctagtg caagcaaagt tgctactttg gacattaaca gcttcaataa
     8041 tggtaataat cttatctgag gagggcgaag tggcggagca attagagttc tttcccgtcc
     8101 agagcccgtg ccggggaatt tgccagtctg acgaacgcgg gttttgtcgc ggctgttttc
     8161 gtagtcggga tgagcgtttt aactggaata aaatgagcga tggcgaaaaa caagaggtgc
     8221 tgcgcctttg tcggcagcga ttaatgcgca aattacgcgc aaataagcca gcgtcatcag
     8281 acgaaccgga gcaaccatca ctcttttgaa cctaatcttg cgtatactcc tctcatatct
     8341 gataagagga agcgattatg gttcagcgta ttactattgc gccgcaaggc ccggagtttt
     8401 cccgttttgt gatgggctac tggcgattga tggactggaa tatgtccgcc cgccagctgg
     8461 tcagttttat tgaagagcat ctggatctcg gcgtgaccac cgtggaccat gctgatattt
     8521 atggtggcta tcagtgcgaa gcggcgtttg gcgaggcact gaaactggca cctcacctgc
     8581 gtgaacggat ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgcgt gaagaaaacg
     8641 tcattggtca ttacatcact gaccgcgatc acatcattaa gagcgccgaa cagtcgctaa
     8701 ttaatctcgc gaccgatcat ctggatctgc tgttaatcca ccgaccagac ccgttaatgg
     8761 atgccgatga agtggcggac gcgttcaaac atctgcatca gagcggcaaa gtgcgtcatt
     8821 ttggcgtatc gaactttacg cctgcgcaat ttgccctgtt gcaatcacgt ctgccgttta
     8881 cccttgccac taatcaggtg gaaatatccc cggtgcatca gccgttactg ctggatggca
     8941 cgctcgacca actacaacaa ctgcgtgttc gtccgatggc gtggtcctgc cttggtggtg
     9001 gtcgtctgtt taatgatgat tatttccagc cgctgcgtga tgaactggct gtggtggcag
     9061 aggagttaaa cgcgggctcg attgaacagg tggtttacgc ctgggtatta cgtttaccat
     9121 cgcagccgct gccaattatc ggttcaggta aaattgagcg cgtacgggca gctgtcgaag
     9181 cagaaacact gaaaatgacc cgtcaacaat ggtttcgtat tcgtaaagcg gcactggggt
     9241 acgacgtacc gtaagcactt ttaggaatag ccgccgttca aaaatgtgtc actggtttac
     9301 acttattcag gaatgcacaa tgaacggagg tcctatgaaa cgttttagtc tggctattct
     9361 ggcgctggtt gttgcaaccg gcgcacaagc tgccagtgaa aaagtcgaga tgaacctcgt
     9421 cacgtcgcaa ggggtagggc agtcaattgg tagcgtcacc attactgaaa ccgataaagg
     9481 tctggagttt tcgcccgatc tgaaagcatt accccccggt gaacatggct tccatattca
     9541 tgccaaagga agctgccagc cagccaccaa agatggcaaa gccagcgccg cggaatccgc
     9601 aggcgggcat cttgatccac aaaataccgg taaacatgaa gggccagaag gtgccgggca
     9661 tttaggcgat ctgcctgcac tggtcgtcaa taatgacggc aaagctaccg atgccgtcat
     9721 cacgcctcgt ctgaaatcac tggatgaaat caaagacaaa gcgctgatgg tccacgttgg
     9781 cggcgataat atgtccgatc aacctaaacc gctgggcggt ggcggtgaac gctatgcctg
     9841 tggtgtaatt aagtaattaa gaggcctgcg taccttgcgg tggtgcttgc tcaagttgcg
     9901 aaagcgagca gtacagccgc cagacaattg ccgccagctc acgggcggca ggttgatgat
     9961 gacgggcaag gctgtcgcaa atccgctgta attcttcaag tgtggccgcc agtgattttt
    10021 gctgaacgcc acgctcactc atcactcccc gcaacagtga aatacagtta tcccgtactc
    10081 gcgataacgg atcggaacgc gattcccagt cgcgcaattg ccagacaaca tgagaacagt
    10141 tcagcagcac tacaccccag cgtaataacc aacggcgagc cagcgcatcc tggctgttac
    10201 tcaactgact gacgtgatga taagtgagcg attcaaattc gctttcactc agtgttggat
    10261 ggcggcttag ctgatcgaca aaatcccggc gcagcgcgcg aatatggcgg cggcttttac
    10321 gagcatccga tcctggacgc agaatggcga acgctagcca agccagcgca acgccaacga
    10381 tttttgccag attatcgtta agaaagtcag caaaatcata caccggggga ttagtgacag
    10441 cgataaacga acccataaaa acaatcagtt gcccccacag tgcggcaaat ttgggcatct
    10501 gcaatttaag aagctgcatt gtcgccagca gtggaaagag aaacagtaaa aattgccaca
    10561 gatcgctaat ctggaccatc aggccaaatt tgaccacaaa gctgaatagc gaaagtaaca
    10621 ccagcgtgcg catcagcagt gacaacgact taaacggtgc tgcgacggcg gagtagagta
    10681 cgcagctaat tgctgccagc gttaatgcat tggcaccggc atcctattgc gatgcaatac
    10741 tccatgcgcc aatcatcatc aacgtacaaa atgtacgcag cccgctccac atagcttcgg
    10801 cgttgtcggt atgacgagcc agagcggatg tccgtgcgag tctggcatga tcatctacac
    10861 cgctttgcag acgatgtaat tcctgactac tttgcagata aaggcggcaa aaataacgta
    10921 gtcgctgcca gaaggcgacg tgccggtagt cggcgacgtt ggtcgggcgt agcggggcga
    10981 taatacgtgc gacggtgtaa gcatctgttt gcgaactggc gagcgccgtc agcaattgtt
    11041 cgagaatttc tcgcgtggca cctggcggtg agggccagtt gagcaacata cgtcgcaggc
    11101 tggagatgac actggtcata cgtaattgct ggtggagtag cgcattaagg cgcgcgtttt
    11161 gctggcgaaa acgatagtgg ctccagaaag cctggatacg cagcaaattc atggtcagta
    11221 tctgcccaat ccccccctca tgtgctgcac gaatggcatc gtttgtttca ggctgccaga
    11281 gtaaactggc atgttccagt aatcgggcgt gcatgttttt caatgcggtt aaaagggctg
    11341 tagcatcgga actgctaggc aggatcatca tcatcatgcc gccgcacaaa ataccgacta
    11401 tcacctcgca aacgcgcgcc tgagcgatat cctacagctg gctggcctca gtaatattaa
    11461 ccatcggaaa ggcgatgatg gcagccgtgt agccgccagt tgaaatgcat acgcgacgtt
    11521 attcgtgaag tgcgcacagg cccaggtaca aaagccaagc cacgccgaca tgctcaatag
    11581 aaaaaaccac ggctcattga gcgtatgccc ggcaagaagc agtgccgcaa tggctcccag
    11641 taaactgcca gcgatgcgtc cgaggctttt gctgatgaca ccgccaacgg tgggaaagct
    11701 aaccactgca gccgaggtca tcgcccaata gggttcatcc agattcaaat aataggcaac
    11761 cgtcagcgcc agacacatgg caatggtatt gcgtaacgca taacgccatt gcgccagcgt
    11821 ggccttgaac cagggcaaat tgcgcaagga ccatgacgat gcgttcatta ttgacctacg
    11881 gcaatgctac aggtggtgcc cgacaccagc gtgacatcgc ctggaacttt atccagcgca
    11941 aagcggacgg gaacgcgttg ggcgagacga acccagggga cgttaggttt cacatccgga
    12001 ataaggcttg agtcactttc aacgctttga tcataaatcg cccgaccgat actggaaacg
    12061 tgaccccgta acgttttgtt atcactatag agggtaattt gtgctggtgc accttcgcgg
    12121 atatggcgca acttggtttc ttcaaaataa ccaatgacgt aaaacgagtg gctgtcgaca
    12181 agggcaaaca gcggtttccc ggtgtcagcg taatcgccga tgcgggtagt gaggttagtc
    12241 acccaaccac tcatcggggc gcgaatttcc gtttgcgcca gttgccattg tgtctgtttc
    12301 agcgtggcct gtgcggcatt aacactggcc tgcatcgctt taacattgag gttagcggta
    12361 tcgagttctt cggcagaaat aaaattttgt gagagatgac gacgacgatt cgcttcgtta
    12421 ttggcttttg cgaggtcaga ttgcgctttc gccagctgag cctgtgcgtt cagctcggcg
    12481 atctgaaagg gcgttttatc gatcgtgagc agaagatccc ccgcgttaac cagttggttg
    12541 tcttttatat tcagctcaac aatacgtccc gacacctgtg gggtgataga aaccttttct
    12601 gcgcgtattt tcccgtcgcg tgtccacgga gattgcatgt aataattcca cagccaccat
    12661 ccggcaagaa ccgcaactat cgctacaatg attattgaga aatacttaat tgttttaatt
    12721 gacatagtta ccacgcaatc agtattgcca gagcaaggca aacgcaaatc gcaaacagcg
    12781 ataaatccat ttacaaggga tgccagatgt caccggcgta gatccagcca cgaagcaggc
    12841 ggtgtacgac aagccagatg acgaatccaa acgcgaaggc tttgaaaaac ggaggaaaat
    12901 agacggacgc accgaacacc agatcttgta agggcaatcc tgttgcattg agcataaact
    12961 tcacaaagta gattccttta cgaccgaata tattgcgtta gattaataaa tattctttaa
    13021 gtgcgaaaaa tttacgcgca atttctgaaa aatatgtctg acggtaacca aatgcagcaa
    13081 tacatttgtt ttagcaataa aattgctgca cactattcta aaagccgcat aatatcttag
    13141 caagctaatt ataaggagat gaaattggaa tcgccactag gttctgatct ggcacggttg
    13201 gtgcgcatat ggcgtgctct gatagaccat cgcctgaaac cgctggagtt aacacaaacc
    13261 cattgggtta cgttacacaa tatccatcag ttacctccag accagtcgca aattcaactg
    13321 gcaaaagcga ttggcatcga gcagccatca ctggtccgta ctctggacca actggaagaa
    13381 aaagggttaa tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg tattaaactg
    13441 acggaaaagg cagagccact gatcagcgaa atggaagctg ttattaacaa aacccgcgcg
    13501 gaaatattac atggcatctc cgcagaggaa ctggagcaac tgattacgct catcgcaaaa
    13561 cttgagcata atatcattga gttacaggcc aaagggtgaa atgaaggggg cgtgtggtca
    13621 gtaacctgac cacacgcaaa cttatttagc gcggagaaac ggtcacctga ctgccattgc
    13681 tggccagtac gacacgttgg cccggagaga aacgagtgtt gccttgtttc tgtaccacca
    13741 tgatggtatt accatcgtct ttacgaattt ccagctcgac accctgcgtt ttgttcattg
    13801 cactctgtac gccctgacca gctacgccac ctgcaacagc gcctgctgca gtagccagag
    13861 aacgcccggt tccgccacca acagtattcc ccaggaaacc accaagaaca gcaccgccaa
    13921 ttgcaccgat aacgttggaa tcatcaccgc cctgaatctg taccggacgt acgttaacga
    13981 tggtgccata gctgacattc tgtacttgtt tcgcttcaga agcggtataa acatcccctg
    14041 acagggtgtc gttattaaca caaccgacaa gagacagacc taccattgaa acaaccaata
    14101 cgcgtttaat cattgaaaaa tctcctgttc accatgaaac gccacgcaag catccctcat
    14161 ggtcaaagta tatggcatat gtgaatccat ggttacatgt tctaccaaat atgagcaaat
    14221 cataatgaaa gtaggatcaa ccaattataa acaaaacatt catgatgatt attgatttga
    14281 agataatgtt ttactaccaa cggtaaaaat cggtcattcc gcatggtatt cacgaaaagt
    14341 ttatgtttga gtggtgcgca aacgtcagat tgaaaaagga tgaactatga aatcgggccg
    14401 ctttattggc gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt tggcgacaat
    14461 agatgaacac cgggtcgcac agctggcaag cttgagctgg ccgatcccgg tgtctctgaa
    14521 acaggctgta ctggatattt gccagggcca gcagcttaca ctttcgcagt ttggacagct
    14581 tgatactcaa ctcgggcgac tttttgctga tgcggtcaat gccttgctta aggaacaaaa
    14641 cctgcaggcg agagatatag ttgcgatcgg ctgtcacggt caaaccgtct ggcatgaacc
    14701 gacgggcgtg gcaccacaca ctttacagat tggcgataac aatcaaattg tggcacgcac
    14761 cggaattacg gttgtcggtg attttcgccg tcgcgatatt gccttgggag gacaaggcgc
    14821 accgctggta cctgcgttcc atcatgcctt gctggctcac ccaaccgagc gacgaatggt
    14881 gctcaatatt ggcggcatcg ccaatctgtc actgctcatt cctgggcagc cggttggcgg
    14941 ctacgatacc ggtcctggta acatgctgat ggatgcctgg atctggcgtc aggccggtaa
    15001 accttacgat aaagatgccg agtgggcacg ggcgggtaaa gttattcgcc cactgctgca
    15061 aaatatgctc agcgacccat atttctcgca acctgcaccg aaaagcaccg gacgcgaata
    15121 ctttaactac ggctggctgg agcgccattt acgccatttt ccgggtgttg atccccgtga
    15181 tgttcaggcc acactggcag aactcaccgc cgtgaccatt tctgaacaag ttttgttgag
    15241 cggtggctgc gaacgattga tggtatgtgg tggaggtagt cgtaatccgc tactcatggc
    15301 gcgtctggcg gcattactgc caggcacaga agtcaccacc accgatgccg ttggcattag
    15361 tggcgatgac atggaagcat tggctttcgc ctggcttgcc tggcggacgc tggcgggatt
    15421 accaggaaat ctgccttccg tcactggcgc aagccaggag acggtactgg gggctatttt
    15481 ccctgctaac ccgtgatatc agagttaact gaatttttct taccgcgtct gccgttacac
    15541 ttggaacgtt agggagggcg tattgccctc cagaccagga aagtcttcgg gaaatgtcta
    15601 tgaaaaaact gttactgatc tgtttgccgg tgcttctcac cggctgtagc gcgttcaatc
    15661 aactggttga gcacatgcaa accgctacgc tggaatacca gtgtgatgaa aaaccgttga
    15721 cggtcaaact gaataatccg cgccaggaag tcagttttgt ttacgataat caactactgc
    15781 atctcaaaca gggcatttca gcgtctggtg cacgttacac tgacggaatc tatgttttct
    15841 ggtcgaaagg cgatgaagcg actgtctata aacgcgaccg catcgtattg aataactgtc
    15901 agttacaaaa tccacagcgt tgagattttt ccaggggcgg cgcacaatag cgtcacccac
    15961 tgacaatccg taaagaaaac catgtctgat aacgacgaat tgcagcaaat cgcgcatctg
    16021 cgccgtgaat acaccaaagg cgggttacgc cgtcgcgatc ttcccgccga tccattaacc
    16081 ctttttgaac gttggctctc tcaggcgtgt gaagccaaac tggcagaccc taccgcgatg
    16141 gtggtcgcta ccgtggatga acatgctcag ccttatcaac gcatcgtttt actcaaacat
    16201 tacgacgaaa aaggcatggt gttttacacc aacctcggta gccgtaaagc acatcaaatt
    16261 gaaaataatc cgcgcgttag cctgctgttc ccgtggcata cccttgaacg ccaggtgatg
    16321 gtgatcggta aagcagaacg actttcgact ctcgaagtga tgaaatattt tcatagccgc
    16381 ccgcgtgata gccagattgg tgcatgggtt tcgaagcagt ccagtcgcat ttctgcccgc
    16441 ggtatccttg aaagtaaatt cctggagctg aagcagaagt ttcaacaggg cgaagtgcca
    16501 ttgccgagct tttggggcgg ttttcgcgtc agccttgaac agattgagtt ctggcagggc
    16561 ggtgagcatc gcctgcatga ccgctttttg taccagcgag aaaatgatgc gtggaagatt
    16621 gatcgtcttg caccctgaaa agatgcaaaa atcttgcttt aatcgctggt actcctgatt
    16681 ctggcacttt attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc ggcaaaggtg
    16741 cagtcgttat atacatggag attttgatgg caagcagtaa cttgattaaa caattgcaag
    16801 agcgggggct ggtagcccag gtgacggacg aggaagcgtt agcagagcga ctggcgcaag
    16861 gcccgatcgc gctctattgc ggcttcgatc ctaccgctga cagcttgcat ttggggcatc
    16921 ttgttccatt gttatgcctg aaacgcttcc aacaggcggg ccacaagccg gttgcgctgg
    16981 taggcggcgc gacgggtctg attggcgacc cgagcttcaa agctgccgag cgtaagctga
    17041 acaccgaaga aactgttcag gagtgggtgg acaaaatccg taagcaggtt gccccgttcc
    17101 tcgatttcga ctgtggagaa aactctgcta tcgcggcgaa caactatgac tggttcggca
    17161 atatgaatgt gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt aaccagatga
    17221 tcaacaaaga agcggttaag cagcgtctca accgtgaaga tcaggggatt tcgttcactg
    17281 agttttccta caacctgttg cagggttatg acttcgcctg tctgaacaaa cagtacggtg
    17341 tggtgctgca aattggtggt tctgaccagt ggggtaacat cacttctggt atcgacctga
    17401 cccgtcgtct gcatcagaat caggtgtttg gcctgaccgt tccgctgatc actaaagcag
    17461 atggcaccaa atttggtaaa actgaaggcg gcgcagtctg gttggatccg aagaaaacca
    17521 gcccgtacaa attctaccag ttctggatca acactgcgga tgccgacgtt taccgcttcc
    17581 tgaagttctt cacctttatg agcattgaag agatcaacgc cctggaagaa gaagataaaa
    17641 acagcggtaa agcaccgcgc gcccagtatg tactggcgga gcaggtgact cgtctggttc
    17701 acggtgaaga aggtttacag gcggcaaaac gtattaccga atgcctgttc agcggttctt
    17761 tgagtgcgct gagtgaagcg gacttcgaac agctggcgca ggacggcgta ccgatggttg
    17821 agatggaaaa gggcgcagac ctgatgcagg cgctggtcga ttctgaactg caaccttccc
    17881 gtggtcaggc gcgtaaaact atcgcctcca atgccatcac cattaacggt gaaaaacagt
    17941 ccgatcctga atacttcttt aaagaagaag atcgtctgtt tggtcgtttt accttactgc
    18001 gtcgcggcaa aaagaattac tgtctgattt gctggaaata atgcattaag tggaaagggg
    18061 gagtgagaaa tcactccccc tggtttttat acagggaaca tgatgaaaaa tattctcgct
    18121 atccagtctc acgttgttta tggtcatgcg ggtaacagtg cggcagagtt tcctatgcgc
    18181 cgcctgggcg cgaacgtctg gccgctgaac accgttcaat tttctaatca cacccagtac
    18241 ggcaaatgga ctggctgcgt gatgccgccc agccatttaa ccgaaattgt gcaaggcatt
    18301 gccgccattg ataaattaca cacctgtgat gccgtgttaa gtggctatct gggatcggcg
    18361 gagcagggtg aacatatcct cggtatcgtc cgtcaggtga aagccgcgaa tccgcaggcg
    18421 aaatattttt gcgatccggt aatgggtcat ccggaaaaag gctgtatcgt tgcaccgggt
    18481 gtcgcagagt ttcatgtgcg gcacggtttg cctgccagcg atatcattgc gccgaatctg
    18541 gttgagctgg aaatactctg tgagcatgcg gtaaataacg tcgaagaagc ggttctggca
    18601 gcgcgcgaac tcattgcgca agggccacaa attgtgttgg ttaaacacct ggcgcgagct
    18661 ggctacagcc gtgaccgttt tgaaatgctg ctggtcaccg ccgatgaagc ctggcatatc
    18721 agccgtccgc tggtggattt tggtatgcgc cagccggtag gtgttggtga tgtgacgagc
    18781 ggtttactgc tggtgaaact gcttcagggg gcaacgctgc aggaggcgct ggaacatgtg
    18841 accgctgcag tctacgaaat catggtgacc accaaagcaa tgcaggaata tgagctgcaa
    18901 gtggtggctg ctcaggatcg tattgccaaa ccagaacatt acttcagcgc aacaaagctc
    18961 tgaaatatct catttcggcc cacaacgacc tgtgggccga tggctattac tttaagcctt
    19021 ccgctgacag tgcgtcttgt acttccggac gttcagccat acgttgcata aatgctgcaa
    19081 tgtgctctaa cccttccaga ttcagtttca ccgcgtatgc ccagcgcaga accgtaaaca
    19141 gataggcatc agcaattgta aatctttgcc cgcagatcca gtgctcatcc ttcagtgtct
    19201 cgttcacata ttgcagcttc ttctccagct gcgcgcgaac tgtcggtttg tactcttccg
    19261 gtgtatcagg gcgaaacaga ggtgtgaaac ctttatgcag ctcggtggcg atgtaattca
    19321 gccattcgat ggttttatag cgggaaatac tgtttaccgg tgccagcaac tggcggtcgg
    19381 ggacgctgtc ggcaagatac tgcataatcg ctacgccttc cgtcagcaaa gtaccgtcat
    19441 ccagcagcaa tgcaggcacc tgccccttag ggttaacggc aaagtaatcg tcaccgtttt
    19501 cgagacgttt tttcattaaa tccacactga cgagggtaaa atcctttccg ctctcacgca
    19561 gggtgatatg ggaagcgaga gagcaggcac ccggtttgta gaacaatttc atcggtaact
    19621 ccttttgtct gtggtttaaa gtatggtagt gcgttgctga acaaaaaaaa agccgctaac
    19681 gaaaagttag cggcttgtaa aagagtttcc ctaaatttta cgctacagca gctttcgccg
    19741 ctttgtctgc agcgtcatcc tgcgtcatac ggtgcagttt aggcgcggtc agcagcatca
    19801 gtactgcaat aatggcagta gcgacaccaa tctgcaagaa tacgcgacca taaacttcca
    19861 gtgacatcag cgggtcggta acgttatccg gcacagccat catacccgca acataaccac
    19921 caatcaggtt tgcaccggca gtggtcagga accagctacc cataatgaag cccatcagac
    19981 gctgcggaac gagttgtgca accattgcca gacccagacc agagatcatc agttccccga
    20041 tgctctgcag gccatagctt gcgaccagcc agcttacaga cacgatacca gcgtcagacg
    20101 cgaatttcgc acccagcggc agaatcagga acgcaccaga acacatcacc atgccgattg
    20161 caaacttggt tggcatcggc agggtatcgc ccatcttgtt atagatagcg gccagaatcg
    20221 gactaccgat gatgatccag aacgggttca gtgcctgata ctgctcaggt tctacggcca
    20281 gacccagaat ggagtgctca acgttacgaa tcgcaaagaa gttcagtgac gttggcatct
    20341 ggctgtacag cacgaagaag ataatggctt cgagcatcag gatgaaggca acgatcattt
    20401 tacgacgcgc agcacctttc atcgcgaagg cttctttacc gaagataacc acgataccga
    20461 aggcaacaac gcccagcgcc atacgcgcaa cttcctgatt gtgcagcagc caggtggcga
    20521 tagcgatcag tgccacaaca ccaataatgg tcagtagcag gttacggtag ttgataggct
    20581 cgaagtctgg ttttgaaccg tactgtttaa cccagcgttg gcagaaggcg aagttaacga
    20641 tagtgatcag caggcctaca acgctcaacg caaacgcaac actccagccg tatttcgcgg
    20701 ccagccacgg cgtagcaatc atagagaaga aagagccgat gttgacggac atgtagtaca
    20761 tggtgaatgc accgtccaga cgcgggtcgt ttttctcata gcatgtagaa agcagagaag
    20821 acgggttagc tttaaacagg ccgttaccga ccgcaatagc cgccataccc atataaacga
    20881 taccggcgtc gtgaccagac caggcaacca gagcataacc aatcgccagc acaatagcgc
    20941 cgagcataat tacgcgttta gtacccagta ccttgtcacc taaccagccg ccgatagcga
    21001 ccagaccata aaccagggca ctaaaggaag agaaaagggt gattgaatcc gcttcagaca
    21061 tacccagttg tttaaccagg taaacagcca taattccttg taggccgtaa taaccaaaac
    21121 gttcccataa ctcaatcgag aagatgagat agaacgcctt cggttgtttg aaagcgttca
    21181 aactgacgct ttcagttggt ttttggtttg cagtggacac ttttacctct ttttttacat
    21241 cccatattaa cgggggtgct ttctcgcgtt atgtctaaaa aacatacgcg caattgttat
    21301 agtgggaggg gaaacggcag gtaatgttca ctatcttgca ctttcagaca agtcctttgt
    21361 aataatttgt tacatataac tgaggcgggg aattcaacca ttccgggaaa tgttatccag
    21421 cgttaaattt ttcaaaaaac gtaacggcag attttttcac tgcactgaga ggggggtgat
    21481 ggcttaacgg cgatatgttc tgctatttag cgtttttact agtgagatag tccagtttct
    21541 gaaaaatagc cagtgtaatg ttttgtaggt caatatttgt tgcgtgtgta aatttaaatg
    21601 attttaacct gattcttgca atgtttttac attgcgacag catcgtgatc ttgatcacga
    21661 atgaatagaa aatttcgcta ttaaaaaaac aattaacctg catttttggt tatccgcaaa
    21721 cggattacct gatgataacg ctaagcaatg gcatcattca cgttaaaaag atgttttaaa
    21781 agaggataaa gaaaggttat caatggggta atcggtgtta ccccttttct tcagatgtca
    21841 actttctctt tgtattcaca aagatcttca ataatacaag agccacagcg gggcttgcgg
    21901 gcaatgcagg tataacgccc gtgcaggatc aaccaatggt ggcagtcgac tttaaactct
    21961 gccggaacca ctttcagtag cttttcttct acctgttcaa cgtttttccc cggcgcaaat
    22021 tgagtacgat tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg ccagccgaat
    22081 gcagtgttta atacgacgtt ggctgtttta cgacctacgc cgggcagggc ttcaagcgca
    22141 gcacgatctt ccggaacctc gccattatgc tgctccagta agatacggca ggttttgatg
    22201 atattttctg ctttgctgtt ataaagccca atcgttttga tataggtttt aaccccttca
    22261 acgcccagtt caagcatcgc tgcaggcgta ttcgccaccg ggtagagttt cgccgtcgcc
    22321 ttattaacac tgacatcggt cgcctgagcg gaaagcagta cggcaatcag caattcaaaa
    22381 ggcgaactga aattaagctc ggtggtggga tgaggattgt tctcacgcag gcgagtgagg
    22441 atctccaggc gttttgcttt gttcatcaga cattccctgt ttcaccgttt ggcaatgcac
    22501 gttcggcagc tgcttcagta cggcgctttt tcattctttc atcaatcagg tattttcctg
    22561 ccagcatcag tcccaggcca ataaatgcac ctggtggcag catcgccagc aggaaagggg
    22621 agtcggtgtg gaaaatctcc acgcgtaata cttttgccca gctacctaac agcgcatctg
    22681 caccgtcaaa caatgtgcca ttgccgataa tttcgcgtag tgaacccagc acgaacatgg
    22741 cgcaggttgc gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc ggaccttttt
    22801 tggcggcgaa ggcttcagcg cggcccacaa cgatacagtt agtgacaatc agcggaataa
    22861 aaatccctaa tgattgatac aggccaaagg cgtaggcgtt gatcagcatc tgtacagcgc
    22921 tgaccaccga ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct ggcgtccagt
    22981 gacgcagcgt cgaaatggtc aggttggtca gcgtcagtac cagcgtagtc gcaagtccta
    23041 aacccagagc attagtggca gtggacgtga ccgccaacag aggacaaagg ccgagcaact
    23101 ggaccagcgc agagttgttt ttccacaacc cctgaacaat aacgtcttta atttcgctca
    23161 cgctttattc tccacaggca ggaagttgag aaagttgtgc cggtaacgtc tgagcgtaca
    23221 atccggcgcg ttttaccgca ttaaccaccg cgcggggagt aatcgtcgcg ccggtgaact
    23281 ggtcgaaatc accaccatct ttcttcaccg cccagtgcgc atcatccgca ccactgattt
    23341 ttttacccgc aaaatgggtg atccagtcag aaaggcgcag ttcgatttta tcgccaagcc
    23401 ctggcgtttc gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta aaatcggctc
    23461 ccaccagcag ctgaatcgca ccggaatagc catctggcgc ggttgcttcc agaacggcgg
    23521 ctaccggttt gtcatcctgt ttggcgatgt aaacccgatg ctcaccttta cctaactctg
    23581 gcgcagttac cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg gctggcagca
    23641 cctgatcaaa taatgccttt tgttgcagac ttgcctgttc agcaatcgtc gttttggtca
    23701 tctggttgat ggccgcagtt aaccctgttg aacccgctgc aaatagcgct aacgtaatgc
    23761 cgtgttttcg gatagttttc agcatggttt accctttgcg atggccgtag acgcgcggac
    23821 gcgtgtagta atcgatcaga ggaaccgtga tgttcgccag caggacggca aaagccacgc
    23881 cgtcaggata gccgccgaaa ctgcggatca accagactaa taagcccgca agcgcgccga
    23941 aaataagacg accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa ataaagaatg
    24001 cgccgagcat ggtcgcacca gacagcagat gaatttgcgg tgctgccagt gtttctggtg
    24061 agaacaacca gcccaacgtt gcgcataacg ccagcgttac taagaagctg aggggaatat
    24121 gccagcgaat cgctttctgc catagcaacc acaggccgcc agccagccag gcgagattta
    24181 cccattgcca accagcgccc gccagaatac cgctgtagat cggatattgc ataatctgtt
    24241 caaccgaatg accggcacgg acagaggttt taaatgtatc cagcggtgtc gcctgactaa
    24301 tgccatcaat acctaagtgt agtgtgttca tatcagcacc actggcggta tgcccgctga
    24361 aaataacctg tatggcgtcg ataaaaccag ggatgttgac cgcaatttca tgtggcggta
    24421 accagctggt catctgcacc gggaaggaga tcagtaagac cacataacca atcattgccg
    24481 gattaaacgg gttttgcccc agaccgccat acaactgttt agcgatgatc accgcaaaca
    24541 ccgtacccag cacgaccatc caccatggcg cgaggggggg aatacttacc gccagcaata
    24601 agcctgtcag caatgctgag ttatctttca acgttgcggc taccgactgc ttgcgtagtt
    24661 tgagtacgag agcttcggct aacagagtac taaccgatgc caacaggatc tgaacgagag
    24721 taccccaacc aaaaaaccac agttgcgctg cgattcctgg cacggctgcg agcaacacca
    24781 acagcataat gcgcgatgtc tggcgctggt tatgggtata aggggagcta gctattctga
    24841 ataccattta gtcctcgttt acaacctttt gctgggcggc ttttttggcc tgagcgcggg
    24901 caatagccgc ggcaactgcc gctttgcgcg gatcaacctg ttcttctggt tctgcgttag
    24961 tctgttgctg ttccagcttg cgcgctttgg cacgggcaat agcggcttcg acggcggctt
    25021 tgcgcggatc aacctgttct tcaggtaccg cattagcctg ttgctgttcc agcttgcgtg
    25081 ctttggcgcg tgcaatagcc gcttcgacgg cagctttgcg cggatcaacc tgttcttctg
    25141 gctccgcatt agcctgttgc tgttccagtt tgcgcgcttt ggctcgggca atagcggctt
    25201 cgatggcggc tttgcgcgga tcaacctgtt cttctggttc cgcattagcc tgttgctgtt
    25261 ccagcttgcg cgctttggca cgggcaatag cggcttcgac ggcggctttg cgcggatcga
    25321 cctgttcttc tggttccgca ttagcctgtt gctgttccag cttgcgcgct ttggcgcggg
    25381 cgatagctgc ttcaacggca gttttacgtg gatcagcaac ggttgctgcg tcgttagttt
    25441 gctgcagttc tgcctgtttc gctctggctt gcgctttacg ggcttcccgt gctgcaataa
    25501 ttgcactgtt atccgggcgt tcgcccgctt taatcacaat aggctgtgta gcctgggcct
    25561 gtttctcttt cacccgcgcc agagcggcag caatcgcatc tttatctttg gctgcaggtt
    25621 gaacggctgc gctcttatgt cgttcaaggc gagccgcttt ttcgcgctcc agacgagcct
    25681 ggcgcgcttc gaaacgcgct ttggcttctg cggcgcgctt ttcttcctga cgaatagccg
    25741 caatttcagc tttttcctga cggaaatatt gcaccagggg aatattgctc gggcaaaccc
    25801 acgcgcaagc cccacattca atgcaatcag caatgttatg cgtggtagct ttatcgtgtt
    25861 gctgaccttt gctgaaccag tacaactgtt gcggcaaaag atccgcaggg caggcgtcag
    25921 cacaggcgct acaccggatg cagctttgtt cttcctgtgg ttcgccaagt tcattggcag
    25981 agggagccaa cagacagttg gtaatcttta cgaccgggac atccagccat ggcaaggtaa
    26041 agcccattag cgggccaccc ataatcacca tttgatcggc agaggggcag aatcgggcat
    26101 cattcaataa atgacgcact ggcgtcccca gccgtgccca gacgttgccc gggcgagcga
    26161 ttgcttcgcc agtcagggtt acaacacgct cggtaatcgg ctcgccatca ataacggcac
    26221 gtttcactgc ataagcagtg ccgacgtttt gcattaatac gccgatatcg gatgaacgcc
    26281 cgccatgtgg aacctgcttc ccggtcagaa tgtaggttaa ttgtttagca ccgccagaag
    26341 gatatttggt tggaatcacc cgcagagaaa tatcgttaga gtccgccagc accgcgcgca
    26401 gcatggaaat cgcctgcggt ttgttatctt caatgccgat aagaatttcg cgtggctgca
    26461 gaatatgcgc aagaatgcga ataccctcta cgacctgagc cgcgcaatcc tgcatcaaac
    26521 ggtcatcggc ggtaatgtac ggctcgcact cagccgcgtt gataatcaac gtttcaatct
    26581 tatctccgcc accctgcaat ttaacgcctg tcgggaatcc tgcaccgccc agcccggcaa
    26641 caccaaactg atgtatgcgc tcgattaact cttcgcgact acgagtgcga taatcggccc
    26701 agccgtcgcg cgggatccag cagtcttcac catcggcatc aataatcacg cttaattcag
    26761 ctaaagctga aggatgagcc gtagagtggg gcgcaatagc cgtaacggta cccgaggtgg
    26821 gcgcgtgaac aggcagcatt ttgccgcgac cacgggtaag cggctggccg cgcaatactt
    26881 tatcgccgac gctaacgcac aactcacctt cagcgccaat atgctgtttc agtggaataa
    26941 caaaacgctg cgccagtggt acctggcgca ggggtgtacc gttggactgg gttttcatct
    27001 ccggtggatg gatgccgccg ttgaaatccc agattttatt ttttctgaat gcagagaata
    27061 acttaagcat ggtgttccac gggaatgata cgcacgggaa tggtgttcag atcccatttc
    27121 caggagtcag gtgtttctgc gaccggttgc aacgagatgc agtgcgtcgg gcacggatca
    27181 acacataaat tgcagcccgt acagagatca ctcattaccg tatgcatggc acgggtagcg
    27241 ccaacgatgg cgtctaccgg acaagcctga atacatttag tgcagccaat acagttattt
    27301 tcatcaataa ccgccaccat ccgcgcaggc gttagctctt gcgcttcgcc atccagcggc
    27361 tgcggctcga cattaagcaa ctcggcaatt tttagcatca cagcttcgcc acctggggcg
    27421 caacggttga ttttttcacc gttacagctg atggcttccg cgtaggggcg acagccggga
    27481 taaccgcact gaccacactg gctctgcggt aagatttcgt caattttctc aacgaccgga
    27541 tcgtcttcca ccgcaaaacg gcgggaggca taacccagaa tggcgccaaa cgccaggccc
    27601 agcaggctca cggcggcaac ggcaatccag atagcattca ttacaacttc accaaaccac
    27661 taaagcccat aaaggccaga gacataagac ctgcggtaat taacgcaatg gcattaccgc
    27721 gaaaaggtgc cgggacatca gccacagcaa ggcgttcgcg gatggcggtg aagagcacca
    27781 tcaccagcga gaaaccgaca gcggcggaaa aaccgtacag cgccgactgc aagaaattgt
    27841 gcccgagatt gatattcagc aacgccacgc cgagcactgc acagttggtg gtgataagcg
    27901 gcaaaaaaat ccccagcaga cggtaaagca ccgggctggt tttgcgcacc accatctcgg
    27961 tgaactgcac gaccacagca atcaccagaa taaatgccag ggtgcgcagg taaatcagat
    28021 taagtgggat caaaatccac gtatcgataa gccaggcgca aatagacgcc agcgtcatca
    28081 caaacgttgt tgccagcccc atgcccatcg cggtttccag ttttttggaa acccccataa
    28141 acggacagag accgagaaac ttgaccagca caaagttatt gaccagtaca gttccgacaa
    28201 acagtaacag gtagtcagtc atttttcggc ctgaaataaa aaagccgcct attatccgtt
    28261 aatccagagc aggcgacaac aggttaactg taaggttatt acggattcac aaacgtcgct
    28321 tttacccgcg acgagcgttt gaaataggag acgatcaatg cggttgccaa caaagtaaac
    28381 agcaactggc gaacggctat gccatcttcc acgggtgaaa aagcgaatgc ttttactgcc
    28441 agcaatacgg aaataagcag ccagataata tagtgtttag gaacacaacg ccgacgttta
    28501 aagaaagcga tggtcaacca cagtgtgtaa taccacatgg cgatagcggt aataaaagaa
    28561 acgccccata agatttgtgt cgtcagtgct tgtccgccga gcgtttgaaa tgtttgcgga
    28621 gaagataacg cagcggtata cagcaacaac gccagcgtcg tactcaacag agcgactaaa
    28681 agccaggcca acgggccaag caaccagcct ccaatacgtt gtggcgttgt ggtggtcata
    28741 ggttctccag aatagcgacg caaacaggca aatctaattt taggggcgtt attataaggc
    28801 ttttataacc gatcgctact ttctttattg gacatagtgc cagacggact tcggtacctg
    28861 gccgaggtca aacaaacgcc cgccagaaac cagctctgcg cgacggtgat cggcagcacg
    28921 atacatattg atcagttcct gatcgtccgt cagggtgtaa ttaagatggt cgtagagttt
    28981 ttccagactt tcgagtgaac tgattttgcg aaattttaat aagtagtcct gaacagtcat
    29041 aaaaggtcca taaaataatt aataccaaag gggtaggtat tattagtgat gataaaattt
    29101 caccaaaagt gaacgcttgt gttattacaa taataagaat gcgctgatgg caaatattca
    29161 gggatgaaaa actgctctga aaagagtaac gcgctttact atccctgaca atcaccaaca
    29221 acatcgaaca agataataaa ttcctggttt aatatccgac aagtgaaaac atgcaaccgg
    29281 acgggcagca tggcgctcta caagcgcaga gcttacttgt gttgtaccga agcactctgt
    29341 tcaggtggct gatagttgtc aatgtgactc gccacgccaa gaagaatgac tgaaacgaca
    29401 agaacgatcc aacctgttaa ttcaataaga cgattcatta cagcccacat ctctcttgat
    29461 tgatccatta acttcagggg gtaaatgtta cttagcaata atagctccag caagattttt
    29521 actgaggttt ttgcgatatt tagcttttgt cgttggaaaa ttcgctattt ttttgactta
    29581 atattaaact gttgagcgtg ggtaaaggat agtgtcaaat agcaatcata cttcaatgag
    29641 aggcaatgac atgagcgaca acatccgtgt tgggttgatt gggtatggtt atgcgagcaa
    29701 aaccttccat gcgcccctga ttgcgggcac gcccgggctg gaactggcgg taatctccag
    29761 cagtgatgaa acaaaagtaa aagccgactg gccaacggtt acggttgtct ctgagccgaa
    29821 gcatctgttt aacgatccca acatagacct gattgtcatt cctacaccca acgataccca
    29881 tttcccgtta gccaaagcgg cgcttgaggc gggtaaacat gtggtcgttg ataaaccctt
    29941 taccgtgaca ctgtcacaag cgcgagagct ggatgcgctg gcaaaaagcc tggggcgtgt
    30001 gctgtctgta ttccataacc gtcgttggga tagcgatttc ttgacgctaa aaggtttgct
    30061 cgcggaaggc gtgctgggtg aagttgctta ctttgagtct cattttgacc gcttccgtcc
    30121 gcaggtgcgc gatcgttggc gtgaacaggg cggcccaggc agcggtatct ggtacgattt
    30181 agcaccgcat cttcttgatc aggccattac gctatttggt ttaccggtca gcatgacggt
    30241 agatttggca cagttacggc ccggagcgca gtcgaccgat tatttccacg ccatcttgtc
    30301 ctatccacag cggcgagtca ttttacacgg taccatgctg gcagccgctg agtcagcacg
    30361 gtatatcgtg catggatccc gaggcagtta tgtgaaatat ggcctcgatc cacaggaaga
    30421 acgtctgaaa aatggcgagc gtctgccgca ggaagactgg ggctacgata tgcgtgatgg
    30481 cgtacttacc cgcgtggaag gtgaggaacg tgtcgaagaa acgctgttga cggtacctgg
    30541 gaattatccg gcttactatg cggctattcg tgatgcgtta aatggcgatg gtgaaaatcc
    30601 ggttccggca agccaggcaa tccaggtaat ggagttgatt gagctgggca tcgaatccgc
    30661 caaacatcgc gcgactttgt gccttgcatg aaaaatggat ggtgcaattt gcaccatcca
    30721 ttttgttact tcgcggcgac tttttctcgc agtgcgcgtt tttcctcagc gctgaggaaa
    30781 gccatttcca gaccattaat ctgtgcctgg cggatttgct cgcgggataa cccagcagct
    30841 ggcgcggcaa cggtatattc gtgaatgata tccactccct gtacgccggg atcgtcagtg
    30901 ttaatgccgg cacgaatgcc atgctcaagg aacgttttca gcggatgtgc agccagctct
    30961 gctacggtgc tggtctgaat attggaggtc agacaggatt caataccaat ttgttgctcg
    31021 gcgagaaaat ccatcagcgc ccgatcttca atggctttta cgccatgtcc aatacgctcc
    31081 gcacccagtt cacgaatcgc ctgccagatg ctttccggcc cggcagcttc gcctgcatgg
    31141 acggtaatat gccagcccgc atcacgcgca aggttgaagt gagaaaggaa cagacttccc
    31201 gggaaaccaa gttcatcacc ggctaaatca agggcggtaa tctggtcacg gtgggctaaa
    31261 aaggcctcca gctcttgctg acaggcggct tcgccgaagg ttcggctcat aatgccgata
    31321 agcttcgcct gcacaccaaa agtgcggcaa ccttcacgta cgccatcgat caccgcttcg
    31381 acaacacccg ctacaggcag cttatgtgcc attgccatgt agcctggtga aaaacgcagc
    31441 tcgacatagt gcaggccgtt acgggctgca tcttcaatgt tttcaaatgc cacgcggcgg
    31501 caggcatcaa gagaggcgag aactttaacg ccccagtcaa gtttggtcag aaagctcacc
    31561 agatcgggtt cgttggcaat gacctgaacg tggggaatca gtgtttccag ggattgtgca
    31621 ggaagcgaga tattatactg gcggccaagt tcaagaatgg tctggggacg aatgttgcca
    31681 tcaaggtggc gatggatatc agttaatggc agggtggtat caatcatggt cgcactcttt
    31741 ttctggatat aaaagtgcgc accattataa aaacaaagca gggtaaaaat ccacgattgc
    31801 gcaacgattt ttacgttgcg caattggggt taacgaacag cgcggatggc gttaattaat
    31861 ccagccacac ctttttccag tttcgaacgt gggcagccgg cattgagacg gacaaaacca
    31921 cgaccttctt caccgtaggt ataccccggc atgatcgcga ctttttcttg ttcgataagt
    31981 gctttttgca acgcgttgtc gtcaatattc aacggacgta aatcaagcca tgccagataa
    32041 gtggattgtg ggatctgcca gttgagttca ggaaacgcgg cgttcatttt atctgcgata
    32101 tacgtcaggt tatctttcag atagatgcgt aaggcatcca gccacggcgc gccttgctga
    32161 taggcggcga tatgggcagt taacgccagt accgaagggg aagaaagccc atcacggcct
    32221 ttcagtgccg ataaataggc atcgcggcta ctgctatttt ctataatccc gtaagcaccg
    32281 gtcagggcgg gaatattgaa acttttcgag cccgacgtta gcaacgccca gtctccgcga
    32341 gccacattac tccagggaat atgcggctgc tcgccccaaa ccatatccat atggatttca
    32401 tcggaaataa cccgcacacc atgacgctcg cacaggtcag ccatgatctc cagctcatcg
    32461 cacgtccaca ctttcccggt aggattctgt gggctacaca ggagcataat tttacattct
    32521 ggtttcgcca acacggcttc caacttgccc atatcgcaaa accaaccatc agcctgcttc
    32581 tctaaagcaa cgggcattac tgtgcgctgg ttaccttcaa tggccttgta aaatgcgtca
    32641 taggcgggtg tgtggatcac cacgccttca cctgtttcag accactgacg aatcagttct
    32701 gaaaccatat agatgacaga agggccatac accaccgtct gagaatcgat ggcggtgtaa
    32761 tgctgggtgg aaaaccagtg ggcaatagca gcgagaaact catcgttttt ccagcggctg
    32821 tagccaaata cgccgtgcat caggcgctga ttcagcgcct ctataatgca gggggcagtg
    32881 gcaaaatcca tgtctgaaat cgtgaacggt aacaggtcag cagtgccgaa acggtcagcg
    32941 acataatccc actgtgtaca ccatgtgcca tgacgatcca cgacctttga aaaatcgaac
    33001 atatcttatc cttatgcctg gacagtatgc atcagaccgg ccatttcatc ttttactgac
    33061 tgaacttgtg gcccgataac aacctgcagg ttatgttgat taagttgtac tacgccaatt
    33121 gcccgattgt ccttcagtgc ctgcacatta acaagcgaca tatctttcac agacaaacgc
    33181 agacgggtaa tgcagttatc gaggctgaca atattgtcgg caccgcctaa tgcttcgagg
    33241 attgcaggaa cgttgtaacc tgatttaccc ggcgcagcgg caacggcttt ttcgattgag
    33301 ctggcaactt cgctatcgcg ccccggggtt ttgagattga agcgggtgat agcgaaacgg
    33361 aagatggcgt agtaaacgac aaaccagatt gccgccacca ctggcaccat gtaccacttg
    33421 gttgacagac catgcaaaat accgaacacc acgaagtcga tgatattgcc gtcggtatta
    33481 ccgatggtga cgccgagcac agacatgacg gtgaagccga ggccggttaa cagcgcgtgg
    33541 atgacataca gaactggcgc tacgaacagg aacaggaatt ccagcggttc ggtagtgccg
    33601 ccaacgacgc aggcgatcag gccagaaatc agcagacctt taattttatg gcgattttcc
    33661 gggcgcgcgc agtgatacat agctaacgct gcagctggca gaccgccgag aaacgcaggc
    33721 attttacctt gcgaaaggaa acgcgtggcg ctttcagaaa aaccgtgagt ggtcgggcaa
    33781 ctcaattgcg tctggaagat ggtcagtgcg ccgctgacgg tttgaccgca gacttcctgc
    33841 gtgccgcctg cgtcggtaaa gcgaattaat gccaccagaa tgtgatgcag accaaacggc
    33901 aacagcagac gttcaccggt accaaacagc atcggtccga aatcacccgc gctgtttatc
    33961 atatggccca agccgctaat acccatggcg aaaatcggcc agactaatgg aatcaccagg
    34021 ccgacaaggc ccatcaccag cgaggagata attggtacga agcgcgtacc gccgaagaat
    34081 gccagcgcat ccggcaggcg gatattatgg aaacgctcat gcagcatcca gacgataata
    34141 ccggcgatca ccgcaccgag gatcccggta tcgatcgact ggatcccaag aatgctctgg
    34201 atgttattgg ctttcagaac cgcggcatcc gtggttggca gaatgccttt attggtcaac
    34261 cagaagttta ccgcgaggtt cattaccgca taaccgacga agccagcgaa tgccgctacg
    34321 cctttatttt cgcgtgccag gcccagcggg atggcgatac agaacatgac aggcaggaaa
    34381 ctaaaagcaa acgagccaat cttactcatc caggtaaaga tagcctgcaa cacggggttg
    34441 cccaggaccg ggatcagggt tatgacatca tggctgctaa gagaactacc aatgccgagc
    34501 ataatgccgc agaacgacaa taatgccacg ggtaacatga aggttttgcc taactgctgg
    34561 aagaactccc acagcgtgac tttcggtgct gttttcgccg tcataaaacg actcctcgta
    34621 gagaacaatc tggattcagt aaattgcgcg agaagataaa acgttttatc aaattttagt
    34681 gaggcataaa tcacattacg caacgataat agcgggtata agataaataa aaggtaaaac
    34741 gttttatctg tcacataatc agggagtagg tcatctgcat ggctaccgcc aaaaaaataa
    34801 ccattcatga tgttgcgctg gctgcgggcg tgtcggtaag taccgtttcg ctggtgctta
    34861 gtggcaaagg gcgaatctct accgccacag gagaacgcgt taacgccgcc attgaagagc
    34921 tgggatttgt gcgcaatcgc caggcgtcgg cgctgcgcgg cgggcaaagc ggcgtcattg
    34981 gtttgatcgt ccgtgattta tctgcgccgt tttacgccga attgacggcc ggattgacgg
    35041 aagctctgga agcgcaggga cggatggttt ttttgcttca cggcggtaaa gacggcgagc
    35101 agctggcaca gcggttttca ctgttactga atcagggggt cgatggtgtg gtaattgccg
    35161 gggctgcagg aagcagcgat gacctgcgac ggatggcaga agaaaaagct atcccggtga
    35221 ttttcgcttc ccgtgccagt tatcttgatg atgttgatac ggttcgcccg gacaacatgc
    35281 aggctgcaca gttgttgacg gagcatctca ttcgcaatgg gcatcagcgg atcgcctggc
    35341 tgggagggca aagttcctca ttaacccggg cagaacgggt gggaggctat tgtgcaactc
    35401 tactaaaatt tggtctgccg tttcacagcg attgggtgct ggagtgcaca tccagccaga
    35461 agcaagctgc ggaagctatc acggcgcttt tacgtcataa cccgaccatc agtgctgtgg
    35521 tttgctataa cgaaactatt gcgatggggg catggtttgg ttt
//
LOCUS       DAJTNN010000020        35013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTNN010000020 DAJTNN010000000
VERSION     DAJTNN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.020"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            32..1270
                     /gene="dsdA"
                     /locus_tag="OO150_000891"
                     /pseudo
     CDS             32..1270
                     /gene="dsdA"
                     /locus_tag="OO150_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OO150_000892"
     CDS             complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OO150_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5515122.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OO150_000893"
     CDS             complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OO150_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5515123.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYVYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            4495..5109
                     /gene="evgA"
                     /locus_tag="OO150_000894"
     CDS             4495..5109
                     /gene="evgA"
                     /locus_tag="OO150_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5515124.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGASAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            5114..8707
                     /gene="evgS"
                     /locus_tag="OO150_000895"
     CDS             5114..8707
                     /gene="evgS"
                     /locus_tag="OO150_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5515125.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OO150_000896"
     CDS             complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OO150_000896"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5515126.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OO150_000897"
     CDS             complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OO150_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5515127.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OO150_000898"
     CDS             complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OO150_000898"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5515128.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSSVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OO150_000899"
     CDS             complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OO150_000899"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5515129.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(14507..15139)
                     /locus_tag="OO150_000900"
     CDS             complement(14507..15139)
                     /locus_tag="OO150_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5515130.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            15435..15710
                     /locus_tag="OO150_000901"
     CDS             15435..15710
                     /locus_tag="OO150_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515131.1"
                     /translation="MKVNLILFSLFLLAYIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(15787..16029)
                     /locus_tag="OO150_000902"
     CDS             complement(15787..16029)
                     /locus_tag="OO150_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5515132.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTEIFMFLAPACGLMIRFMVGYGRR"
     gene            16382..17302
                     /gene="lpxP"
                     /locus_tag="OO150_000903"
     CDS             16382..17302
                     /gene="lpxP"
                     /locus_tag="OO150_000903"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5515133.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            17658..17729
                     /gene="ypdK"
                     /locus_tag="OO150_000904"
     CDS             17658..17729
                     /gene="ypdK"
                     /locus_tag="OO150_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5515134.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OO150_000905"
     CDS             complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OO150_000905"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5515135.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRQDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            19409..21105
                     /gene="ypdA"
                     /locus_tag="OO150_000906"
                     /pseudo
     CDS             19409..21105
                     /gene="ypdA"
                     /locus_tag="OO150_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
     gene            21120..21854
                     /gene="ypdB"
                     /locus_tag="OO150_000907"
     CDS             21120..21854
                     /gene="ypdB"
                     /locus_tag="OO150_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5515136.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            21867..22724
                     /locus_tag="OO150_000908"
     CDS             21867..22724
                     /locus_tag="OO150_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5515137.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNSQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22727..25222)
                     /gene="ptsP"
                     /locus_tag="OO150_000909"
     CDS             complement(22727..25222)
                     /gene="ptsP"
                     /locus_tag="OO150_000909"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5515138.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRESDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFCTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(25247..26284)
                     /gene="ypdE"
                     /locus_tag="OO150_000910"
     CDS             complement(25247..26284)
                     /gene="ypdE"
                     /locus_tag="OO150_000910"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5515139.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGTRSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGVRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            complement(26284..27369)
                     /gene="ypdF"
                     /locus_tag="OO150_000911"
     CDS             complement(26284..27369)
                     /gene="ypdF"
                     /locus_tag="OO150_000911"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5515140.1"
                     /translation="MTLLASLRDWLKEQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSSYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(27384..28631)
                     /locus_tag="OO150_000912"
     CDS             complement(27384..28631)
                     /locus_tag="OO150_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5515141.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGVIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(28653..28979)
                     /locus_tag="OO150_000913"
     CDS             complement(28653..28979)
                     /locus_tag="OO150_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5515142.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(29198..30163)
                     /gene="glk"
                     /locus_tag="OO150_000914"
     CDS             complement(29198..30163)
                     /gene="glk"
                     /locus_tag="OO150_000914"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5515143.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            30367..31623
                     /locus_tag="OO150_000915"
     CDS             30367..31623
                     /locus_tag="OO150_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5515144.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTINIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            31738..32064
                     /locus_tag="OO150_000916"
     CDS             31738..32064
                     /locus_tag="OO150_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5515145.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERSYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(32205..33443)
                     /locus_tag="OO150_000917"
     CDS             complement(32205..33443)
                     /locus_tag="OO150_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5515146.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKTLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVLPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            33779..34981
                     /gene="nupC"
                     /locus_tag="OO150_000918"
     CDS             33779..34981
                     /gene="nupC"
                     /locus_tag="OO150_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5515147.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         9233 a         8697 c         8081 g         9002 t
ORIGIN      
        1 gtcacggcga actgcctttc aaggttgaag tggcacgacc tcattggctg aaggtttacc
       61 ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc
      121 accctatctg gcaaaagcat ttgctgaaac tgctgccact ggggggatta ttgaatcaga
      181 actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag
      241 cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccagctcca taaaagcacg
      301 cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct
      361 gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca
      421 atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc
      481 ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc
      541 gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt
      601 cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa
      661 ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc
      721 ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt
      781 tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca
      841 ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt
      901 acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct
      961 tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gtgcgtctgc tggatggctt
     1021 ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg
     1081 tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg tgtgtgcatc
     1141 agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata ccactcatct
     1201 ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa
     1261 aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga gcgatgctgc
     1321 cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct tttatcatca
     1381 cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat
     1441 aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt
     1501 tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt tatccataat
     1561 ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg
     1621 gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga
     1681 caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc
     1741 tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa
     1801 ctgcggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta
     1861 atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt ctattttgtt
     1921 gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg gcgcataagc
     1981 aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac
     2041 gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa tggtgaagtt
     2101 acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac
     2161 taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa
     2221 ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa
     2281 cagggtcagt cctggtagat tcattttgac cggtgaagtc tcagtttctc ttcctttaag
     2341 taaggttaag cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca
     2401 accccagcta aagttatcac aaatataacc gcccaatatc ggcccacata tcggagcgat
     2461 aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggcg gataattcct
     2521 taataataaa ctctgtgaca gtggaattaa cggccccgcc attaacccct gaacgactct
     2581 aaaaaatatc agcacatcaa gattggtcga taggctacac attaatgaag acagcgaaaa
     2641 aaaggttact gaaagtaaaa ataatcttaa ttcgcctatt ctttgtgcca acctgccagt
     2701 aacagggatc gcaatggcat ttgctacacc aaacgaggtg ataacccagg tgccttcgtc
     2761 tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc
     2821 caacatttgc ataaatgtcg ctaatgacaa tgcaatagtg acgcaccata acgtcccacc
     2881 ggttaatggt gccggagtcg atttagtgat tgccattaaa gttgtccatt atgcgaaata
     2941 atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa
     3001 gccggcatgg aggtcacggt tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc
     3061 gtatcaatag ttgctgtcat cgataaacca atacgcaagg gatgttccat gagttctttt
     3121 ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca
     3181 ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga
     3241 ccatgaaaca caacattttc accataaaga tcgctgataa tattgaccga ttgaccaatc
     3301 cgcacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt
     3361 acgacagcca ttaacgattg tccggggctc actgtttcgc cgacctgaac acttctctgg
     3421 gcaatatagc cggtaaccgg actcttaata tccgtacgtt taagcgccaa ccaggcttct
     3481 ttagttgcat ccgccgcttc aacgacttgt ggctgacggt ttaatggtgt gttcattact
     3541 aaagctttat tcgctttata agcctggata gcggcattca atgccgcttt gctacttatt
     3601 aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaacggc
     3661 actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg
     3721 gcactgtatt gtttatcctg taagtatagt ttattcgttt gccgaacaat atttgccaga
     3781 ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg
     3841 tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact acctgagact
     3901 tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct
     3961 tctaattcca ttgaccaata gacataggca cctgaaaacg caataaataa aactaccgcc
     4021 aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat ctgttccact
     4081 attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc attcattctt
     4141 catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa
     4201 atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg
     4261 cctgtaggat tagtaagaag acttatagtg ctaacttgaa actataaatc atcggtacaa
     4321 cccctgattt tattgttgac atttcattta tgccgactat ttatatggta tacttgtcga
     4381 attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga tgacgcctta
     4441 tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac
     4501 gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa
     4561 aacgatattg aaatcttagc ggagttgact gaaggggcaa gcgccgttca gcgggtggaa
     4621 acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag
     4681 gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattattgt ctccgctaaa
     4741 aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggatt cgtgagtaaa
     4801 aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat
     4861 ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac
     4921 tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat
     4981 gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg
     5041 atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa
     5101 atcggctaat cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc
     5161 gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg
     5221 tgtcacactt gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa
     5281 aaatcttgtg attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca
     5341 gcaacgggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat
     5401 caaattaaca ctccgggaat acgccgatca tcaaaaagca atggacgcgc tggaagaagg
     5461 tgaagtcgat atagtgttat cacatttagt tgcttcgccg cctcttaatg atgacattgc
     5521 tgcaaccaaa ccactgataa ttacctttcc ggcgctcgtc accacccttc acgattcaat
     5581 gcgaccgctt acctcatcaa aaccagtaaa tattgctcga gtagcaaatt atccccctga
     5641 cgaggtaatt catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca
     5701 ggcattagca tccgtctcag ccggacagaa tgattacttt attggtagta acatcattac
     5761 cagcagtatg atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc
     5821 accgcgtcaa tataattttt tcttgaccag aaaagaatct gtcgttctta atgaagtact
     5881 caatcgattt gttgatgctt taacaaatga agttcgctat gaagtttcac aaaattggct
     5941 tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca
     6001 gtggattaag caacatcccg atttaaaggt gctggaaaat ccttactctc ctccctattc
     6061 tatgacggat gaaaatggct cggttcgggg cgttatgggg gatattctta atattattac
     6121 cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc atgccggaac
     6181 acagcttaac cccggtggat gggatataat acctggcgct atttatagtg aagatcgaga
     6241 aaataatgtt tcatttgcag aagctttcat aacaacgcca tacgtttttg tcatgcaaaa
     6301 agcacctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta
     6361 tgagctgcat tcgcaattaa aagagatgta tccggaggtt gagtggataa aagtcgataa
     6421 cgccagcgct gcatttcaca aggtcaagga aggtgaactt gatgctctgg tcgcgacaca
     6481 gctaaattcg cgttacatga tcgatcatta ctatcctaat gaactgtatc attttcttat
     6541 tcccggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa
     6601 ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga
     6661 aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctctata gcgagcaatt
     6721 ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat ggggattcta
     6781 cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc
     6841 attccgaaaa gcactctcgg attccttacc aaatccaact tatgttgtaa actggcaagg
     6901 taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa
     6961 tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca
     7021 tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga
     7081 taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc tgccagccag
     7141 cgatcatgct gtttatattt gtggttggca agatattacc gagacgcgtg atttaattca
     7201 tgcactcgaa gtagagagaa ataaagcgat caatgcaact gtcgcaaaaa gtcagtttct
     7261 ggcaacaatg agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact
     7321 tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc
     7381 caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc
     7441 gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg
     7501 tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt
     7561 tcctgatcat tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt
     7621 actgagcaat gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt
     7681 tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt
     7741 atcgcaggaa gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca
     7801 gcaaacagga tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg
     7861 cgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt
     7921 agaaattagc cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc
     7981 tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg
     8041 ccagctaaat ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca
     8101 caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga
     8161 tggttttgag ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct
     8221 tacagccaac gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg
     8281 tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt
     8341 tgcgcatatt gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc
     8401 gaacgatcta caactgatgc aggagattct catcactttc cagcatgaaa cgcataaaga
     8461 tctacccgct gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat
     8521 tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca
     8581 tcagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa
     8641 ctctgtaaaa gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa
     8701 tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac
     8761 atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc
     8821 ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt
     8881 cccccggctt caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc
     8941 acacttaata acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca
     9001 gctgcctgcg tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg
     9061 cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg
     9121 cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta
     9181 ttgaaaacat caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc
     9241 cctgtcgcga tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc
     9301 gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt
     9361 ccgctgaata aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc
     9421 ggagcatcag gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg
     9481 taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg
     9541 aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct
     9601 gggcgaaaat tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta
     9661 tcgtaatcat tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa
     9721 tagagtgact gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc
     9781 ggctcaactt taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca
     9841 ttaaggacat gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta
     9901 tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg
     9961 taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc
    10021 cgtgacgaca aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac
    10081 attaaaccgg ctggcaataa taattccgga gaatgccggc ggtaatgcgc ctgccagtac
    10141 catcatctgc agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag
    10201 tggcatcaga atcagcttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt
    10261 atgtgcagcc agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat
    10321 cagattaaag gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt
    10381 tgccagaaca ggtgcccata ctactggctc ctttgccgca gaaattaatg cactcagatt
    10441 actattcttc ttaccatccg ctcctgaaga aggattcagc aaatacagac caataggaat
    10501 agtaattgcg ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc
    10561 accataaata ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacctgcaat
    10621 taatgcacat acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccgaacca
    10681 ggagaaaaag aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc
    10741 aaaaatcatt tccctgtttg cccgagtaat agatacaaat agagccgcag gaagcgcgta
    10801 gtttaatacc agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc
    10861 gctaaaataa cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt
    10921 tagcataata gtatttccga taagggtccc ctcaccatcg cataatgagt tggcaagagg
    10981 agacgatgtc agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc
    11041 actttcagtt cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg
    11101 aataccggtg gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc
    11161 acgaaacgca tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggagatgg
    11221 tgcaccagcg ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat
    11281 aacgatcgtc accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc
    11341 aaaagcacta tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat
    11401 ggcatagccc ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg
    11461 tttatacata tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa
    11521 ataaatatcc tggttctcgc gcaatacgtc gcgcacagaa cttaatgcat taaagtaatt
    11581 taatggttga gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg
    11641 gatatttaaa atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc
    11701 cagcatacct tgcatactgg atgcaatatc accaatgact ggcacagcaa tggggcggtt
    11761 gctgtcaatt tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc
    11821 ttttttaccg tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc
    11881 atttgccagg gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat
    11941 ccctttcgcc atagacattg gcaggaatgg aatctgagca ctttcaataa attcacgaag
    12001 ctgttcatca gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc
    12061 ttttgctaaa agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga
    12121 cggattttca actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag
    12181 gacatttgct ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat
    12241 agcgcgtgcc aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc
    12301 atacggtttt gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac
    12361 gatcgcgcgg tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt
    12421 ggccaatgcc gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg
    12481 tttttgggta agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa
    12541 accaatataa cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc
    12601 tacaacacca taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat
    12661 accatctgtc atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg
    12721 ggagccagaa gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca
    12781 tcgtcgctat aacctagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca
    12841 gctttaatat ccggcgtaaa agcagagaat ttcattggac aaccaacggt cagatatttt
    12901 ccacgcaacg gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt
    12961 gaaatttctt tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc
    13021 accgcttcat gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa
    13081 atatgtggct gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg
    13141 ccgatggctt tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg
    13201 ttcggatcgg tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct
    13261 gcattaccac cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct
    13321 tccagataac ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc
    13381 aatacggcat cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc
    13441 agcaaagcag caagcaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct
    13501 gcagcgctta ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct
    13561 gcctgagcaa cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct
    13621 ttgatcgaac caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc
    13681 atgtgatcaa tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc
    13741 ttttccatta cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta
    13801 ctgttaagca tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc
    13861 gttacgtcac caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc
    13921 atttgagtac aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga
    13981 agtggagttg acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat
    14041 ttaaggcgta atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat
    14101 ttttaaaatc ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg
    14161 catggtaagt agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga
    14221 gagagtcgcg acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa
    14281 gtaagggtat gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg
    14341 tgatgacacg ggattattgg ggtttacttt gaggaagata aaatgcgtgt ttatagaacc
    14401 gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg
    14461 gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca
    14521 gtgcttcgct gtcgacgatg attccatctt ccgcaccttt cagcgccagg tttgcctcgt
    14581 agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa
    14641 gcggcatcag atattggttt tccattacgc caacaccggc caggcgaagt tcttccattg
    14701 cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt
    14761 cgggagttgc tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt
    14821 catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat
    14881 ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa
    14941 acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac
    15001 gctctgctgc tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta
    15061 ccggtgcatt atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag
    15121 ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc
    15181 atattgggtg agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct
    15241 tacagttgta gttattatcg caagaagtat tttagtctta caattgtagg aatctgcttc
    15301 ctgtcgcaga gaactattct tatgtaccac aaagagatgt catttattat atcgatgtaa
    15361 atattgatta attatatgta aatacagcca taacatcgta ggttctcagt gaaccatttt
    15421 caggataact ttctatgaaa gtaaacttaa tacttttcag cttattttta ttggcctata
    15481 ttatggcatg caatgttttt gcattttcca tttctggtgg tgtaagtgag aggagctata
    15541 aagagactga aaaaacatca gcgatgacga ccacacactc tacaaaactt cagccatcac
    15601 aggcgatttt gtttaagatg agagaagatg cgccaccatt aaacctcaca gaagaaatca
    15661 cgcctactta tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac
    15721 atggcaggtg aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gagccatcct
    15781 gacccactat cgcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc
    15841 aagaaacata aatatctccg tcactagttc attaccgcca gttaaccaca tggtgaagta
    15901 ataaatgagt gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt
    15961 cagtacctga ccgatgtagc cgcttacgca gacaatacac agggcgagaa acatccataa
    16021 ataaatcata gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata
    16081 gtaagacaat ccttaacctc tggcaatttt tgctaagcat caatgaaaac agcatgttaa
    16141 atgcaagact gttgtgtacg gaaaaatatt tactttgcac gattaataat cagtagctga
    16201 aagcagtcag cgagtgataa agtacagata ccgcattcat gctcctcgtg gttatgtcct
    16261 gactagtctt tacacacttt acaggaacca ttgtcgtaca tgatggccca accaattgag
    16321 tgtaccgctg tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac
    16381 tatgtttcca caatgcaaat tttcccgcga gtttctacat cctcgctact ggctcacatg
    16441 gtttgggctt ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct
    16501 cggcacgcgt attggcgcaa tggcgcgacc attcctgaaa cgtcgtgaat ctatcgcccg
    16561 taaaaacctg gaactttgtt tcccgcagca ttctgcggaa gaacgcgaga agatgattgc
    16621 cgaaaacttt cgttcactcg gcatggcgct ggtagaaacc ggcatggcat ggttctggcc
    16681 cgacagtcgc gtacgtaaat ggtttgatgt tgaagggttg gataacctta aacgcgcaca
    16741 aatgcaaaat cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg
    16801 ccgcgtgatg ggactgtgcc aaccaatgat ggctacctat cgtccacata ataatcagct
    16861 gatggaatgg gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa
    16921 taatctgcgc ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga
    16981 tcaggattat ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc
    17041 cacaaccaat ggcacctatg ttctctcccg tctctctggc gcagccatgt tgaccgtaac
    17101 gatggtaaga aaagcggatt acagcggata tcgtttgttc atcaccccag agatggaagg
    17161 ctacccgaca gatgaaaatc aagccgctgc ctatatgaac aagattatcg agaaagagat
    17221 catgcgcgca ccggagcagt acctctggat ccactgtcgc tttaaaacgc gcccggtggg
    17281 agaatcgtcg ttgtacattt aacaaactgc tcttactctt tccctcctca aatcggccat
    17341 aataacctca tggtttttat ggcttttttc ttaaggtttt ttccttcgct gaatatctaa
    17401 ttattaccgt cagaaattaa actgtcgccg atatgagaca cttgtaagtt actaaaagta
    17461 aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc
    17521 agtttagctc agaattctgg ggtgatgccc acggtaaaat acagggcttt taaggtcgct
    17581 ttgcccagtg tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagcaatt
    17641 acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt
    17701 gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca
    17761 tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt
    17821 gctgctggcg ggtagtaaac cgtcggcacg gaacatcgct ttaatccctc tgatggcctg
    17881 acgaatacgg tcgcggtttt caatcagtgc aaagcgaaca tgcgtatcac catagtcgcc
    17941 aaagccaatc cccggcgaga cacagacctt cgcttcgtta agcaatttct tggcaaatac
    18001 cagcgagccc atggcagcat acggttccgg tattttcgcc cagacataca tcgaagcctt
    18061 cggcatttca actatccagc ccgcttcatg cagcccttta accaatacat cacggcggcg
    18121 tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc
    18181 cgccacctgc aacggcgtaa aggtgccgta atcgtgatag cttttaatac gagccagggc
    18241 gctgaccagc gttttgttgc caaccataaa gccgatacgc cagcccgcca tgttgtagct
    18301 tttcgacagc gtaaagaact cgactgccac atcgcgtgca cccggtacct gcatgattga
    18361 cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcgtgga ccaccagcac
    18421 atcgtagcgt ttcgccagcg ccacaacctt ttcgaagaac tccagctcca cgcattgcgc
    18481 ggttggattc gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat
    18541 ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg
    18601 cgccccggca atcaccgcgc cataaatatg gatcgggtaa cttggattcg gcaccagcac
    18661 cgtgtcacca tgatccagcg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat
    18721 agtgacgatg gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg
    18781 ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtcctg
    18841 gcgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt
    18901 acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc
    18961 ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg
    19021 agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc
    19081 cacaatggtg gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa
    19141 catttttatc aatggcttta aatgaggaac tccacttcaa aaacaggaaa tttttctacg
    19201 ctaacaagaa aattaccacc aattttatta acattataat ttcaataaat ttaccagcca
    19261 gaagtgcatt atcaagagat acattgctaa tacaccattt atctttcaat ccccttttaa
    19321 atttatggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata
    19381 gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt
    19441 cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta tccgcctgtt
    19501 tcgcgagctg ttgcacaagt cggcacactc cccaaaagag ttgcttgctg ttaccgccat
    19561 tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag gctcgctggt
    19621 gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt gggtaggcat
    19681 cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg gcgtgacggc
    19741 gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga taaacctgaa
    19801 aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt gtgagacgtt
    19861 gaccatgatt ctggtaattg tctgggcacc aactaccgcg ttggggatcg atatcgtctc
    19921 taaaatcggc attccaatga tcctcggtag cgtctgtatc ggctttattg tgcttctggt
    19981 gcaaagcgtt gagggcgaaa aagaggccag cgccgcgcgg caggccaagc tggcgctgga
    20041 tatcgccaac aaaacgctac cgctgtttcg ccatgtcaat agcgagtcat tacgcaaggt
    20101 ctgcgaaatt atccgcgatg acattcacgc cgatgcggtg gcgattacta ataccgatca
    20161 tgtgctggcc tatgttggcg tgggtgaaca taactatcag aatggcgatg acttcattag
    20221 cccgactacc cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca atgatgaagc
    20281 ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga aaggcgttgt
    20341 gaccggaacg ctgaaaattt actactgcca cgcgcatcag ataacctcgt cattacagga
    20401 aatggcggtt ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac gcgccgagca
    20461 gctacgtgaa atggcaaata aggcagagct tcgcgccctg caaagcaaaa ttaatcccca
    20521 ttttctgttt aacgctctga acgctatttc atcgtcaatc cgtctgaatc cggataccgc
    20581 tcgccagttg atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagacga
    20641 tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca
    20701 ggcccgcttt ggtgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg
    20761 cattcccagc ctgctgatcc agccgttggt ggagaacgcc attgtccacg gtattcagcc
    20821 ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggaaatc gggtacgcat
    20881 tgcggtgcga gataccggca cggcatcgat ccaaaggtga ttgagcgggt cgaagcgaat
    20941 gaaatgccgg gcaataaaat tggcctgctg aatgtccatc atcgcgtgaa gttattgtat
    21001 ggcgaggggc tgcatatccg ccgcctggag ccggggacgg aaattgcgtt ttacattcct
    21061 aaccaacgca ccccagtcgc ctcacaggct acgttattgc tttgagccgg agtgatattg
    21121 tgaaagtcat cattgttgaa gacgaattcc tggcacaaca ggaactgagc tggctaatta
    21181 aagagcacag ccagatggag attgtcggca cctttgacga cggtctggac gtgttgaagt
    21241 ttttgcagca taaccgcgtc gacgccattt ttctggatat caatattccg tcgctggatg
    21301 gcgtgttgct ggcgcaaaac atcagccagt tcgcccataa accgtttatt gtgttcatca
    21361 ccgcgtggaa agaacatgcg gtagaagcgt ttgaactgga ggcgtttgac tacattctca
    21421 aaccgtatca ggagtcacgt attaccggga tgctgcaaaa actggaagcg gcctggcaac
    21481 aacagcagac cagcagtacg cctgccgcga cggtaacgcg tgagaatgac accattaatc
    21541 tggtgaaaga tgagcgaata atcgtcacgc caattaacga tatctattac gccgaagcgc
    21601 acgagaaaat gacctttgtc tatacgcggc gtgaatccta cgtaatgccg atgaacatta
    21661 ccgaattttg cagcaaactg ccgccgtcgc attttttccg ctgccatcgc tcattttgtg
    21721 tcaatctgaa caaaatacgc gaaatcgaac cgtggtttaa taacacctac attctgcgac
    21781 tgaaagatct ggattttgaa gtgccggtca gccgcagcaa agtgaaagaa tttcgccagt
    21841 taatgcatct ttaatcggga actttcatga aagcaccagg cttgcccgcc gatcaacaat
    21901 ttttcgccga tctgttcagc ggcctggtgc ttaactcgca actactgggg cgcgtctggt
    21961 ttgccagcca gcctgcctcg ttaccggtgg gcagtttatg tattgatttt ccccgtctgg
    22021 atatcgtgct gcgcggcgaa tacggcaacc tgctggaagc aaagcagcag cgaatggtgg
    22081 aaggagaaat gctgtttatt ccggcacgcg cggctaattt accgatcaac aacaaaccgg
    22141 tgatgctgtt aagcctggtg ttcgcaccga cctggcttgg gttatcgttt tacgatagcc
    22201 gcaccacgtc gttgttgcat cctgctcgcc agatccagct tcccagcctg caacgcggtg
    22261 aaggtgaagc gatgcttacc gccctcactc atcttagccg ttcgccgctg gagcagaata
    22321 tcattcagcc actggtgtta agtttgctgc atctttgccg caacgtggtg aatatgccgc
    22381 cgggtaattc gcaaccgcgc ggcgattttc tctatcacag catttgtaac tgggttcagg
    22441 ataattatac ccagccgctc acccgcgaga gcgtggcgca gttttttaat atcacgccca
    22501 atcatctgtc aaaactgttt gctcagcatg gaacgatgcg ttttatcgag tatgtgcgtt
    22561 gggtgcgaat ggcgaaggcg aggatgattt tgcagaaata tcatctgtca attcatgaag
    22621 tggcacagcg ttgcggtttt ccggatagcg actatttttg tcgcgttttc cggcgtcagt
    22681 ttggtctgac gccgggagag tacagcgccc gttttcaggg ctaacgtcag aaggttaatt
    22741 ctgtttccag cagcgtcagg atactttgtg catcttgcgc ggcaaacaac gactggcgga
    22801 agtttttatt caccagttta cgcgccagct gcgagaagac tttcacatga ttcatccctt
    22861 cgttagcacc cagcgtcagc atgatcacca gttcgacttc gcccatttct gactgccagt
    22921 caatcggttt cgccagccgg gcaatgctga tactggaatg acggatccac tgagatttgg
    22981 tgtgcgggat cgctacgcca aaaccaacgc cggtggtaac aatctcttcc cgctgccaga
    23041 catcttcttc cagctcaaac ggatgttcag tgcgcccgtt aacgccgagg ttgccgcaca
    23101 ggaactggat cgcctgctct ttattgctaa aatcctgatc aacaaagata ttctccagcg
    23161 ccagcagtgg gcgaacgtct tcttccggcg taaaggcggt gagtaacgct tcaatttcct
    23221 gcgcactgcg gcattcacat gcctgacgcg ccagttcccg acacgcctcg ctatccagtt
    23281 gacgaagctg gcttttcacc gccggaatac gcgggctact catactcagc tcatccaggc
    23341 ccagcccaag cagtagcggc agataacggc tttcaccgcc cagttcaccg caaatgccta
    23401 cccatttgcc ccgctgatgc gcagtggtaa ctatttgctg caacatgcgc aggaacgatg
    23461 gcgtaatcgg gttatatagc ggcgatacgc gcgggttatt acgatcgacc gcatacagat
    23521 actgggtcat atcgttggag ccgatactga agaaatcgac ctcatcgcag aagtggtcga
    23581 tgatgtagca caccgacggc acttccacca tgatcccaag cgtaatcgtc tctgcatgac
    23641 gcaggccatc gcgcttaagc tcaacgatcg ctttttggat ctcgcccttc acccataaga
    23701 tctgatcgag actgtggacc atcgggatca tcaactgggc gttgccaaaa ctggcggcgc
    23761 gcaaaatcgc ccgtagttga gtgcagaaca ggccagcaaa ttccgggtaa atacgtaccg
    23821 cgcgatagcc gaggaacggg ttctcttcct ggggaatatt cagataagga atgcttttat
    23881 cgccaccgat gtccattgtg cggaagataa tcggcttgtc gcccgctgcc agtagcacct
    23941 gctggtaggc ttcaaattgc tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca
    24001 tttcagtacg gaacagtcca acgccttccg cgccgttggc aaacgcgcct ggcgcttcca
    24061 gagcggtgcc gatattcgcc gcaatatcaa tacgcttgtt atcacgggaa taggccagct
    24121 gcgcggcggc ctgcgcctgt tgtttttggc gtttatccgc cagcgtctgt gcgacctgat
    24181 aataaccgct caccgcgtca ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca
    24241 ccgccggttg cccggcataa cgggcaatcg catccagcgg caggccgctc agtaccggga
    24301 tcgccgaggc gcgggccaga atcagggtat gcgaggtgcg cccagttttc tccagaatca
    24361 tgcctgcaag attttttaaa tcgaggctca aaaactggct tggggttaaa tcctcggcta
    24421 ccagaatagt cggtttttcc agcaccagat tgttgcgcgg cttcagttcc ggccaggtga
    24481 tatgcagcaa ctgttcgcta atatcgcgaa tgtcgctaac acgttcgcgc agataatcac
    24541 tggcagaagc agaaagtttg gcgcaaacct gctccatatt gctgatgatc gccgccccca
    24601 gcccttgatg ctgttctgtc atcagacgac ggatattgcc cgcgaattca tcatcctgaa
    24661 tcagcgacaa atgggcgcta aggatagttt tgctttcgcc gtcactctca cgcaattgct
    24721 ggttcagttg ctcggcaagc gttgcaaggc tgtgctccag ccgggtggaa tcttgcgcac
    24781 tggcggggat tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca
    24841 cgccgacgct gcttgccagc acattgccgt acagtaaatc cgggttcagg cggcttaatg
    24901 aacgcggcag cggatgcgcc gtcagttctg cctgcgttgg ctggatgctg tcgctgtcga
    24961 taaagcgcac ctggatgtac tcttccaaca cgcgctgcgc ctgctcttca tcgctgccgc
    25021 taatgttcag gctgcaactg tcattaaata aagtaccggt gccaatcagc gccagcgagc
    25081 ttttcgcatc ggcctttgcg ttctggcgat ggttgataaa ggtgatttcg ctttgccact
    25141 ggctgcactg ttctttaagt tcccacgccg gacgagcatg aagaccgttt ggcaaaggac
    25201 agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat ctgaaatccg
    25261 tcagttgaac aaccgtctca cgcgtaagac gtttaataag ggcagataaa agctgctgca
    25321 tctgcaaaat gtcacggcaa tcggcaatcg atgcggcgca atgtccgtgg cgggttgctg
    25381 gccccatcac cactgtaggc acgccggtgc cggttaagtg caccgctccg ccgtccgtgc
    25441 cgccgttgct gaacatatct gcctgtaacg gcacgccaat ttctgccgcc acggtttcga
    25501 tccacgcggt aagttttggc ggcgcaatca gcgacttgtc gcttaacacc agcatcggcc
    25561 cgttacaaat ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg
    25621 tatcaagcac aatggcgaca tccggcgaca ccgcgcgggt ggcagtttgc ccgccgcgta
    25681 cccccacctc ttcgctggaa ctggccacca gccacacttc cgcaggcagt tcagcgctgt
    25741 gtagttcgcg cagtaacgtc accagcaggt agcaaccgag gcggtcatca aaggctttcc
    25801 ccatcacccg ctggtgaggg agaacctgaa aagttgtatc aaaggtgacg cgatcgcctg
    25861 gacgaattcc cgcctgcatc acttcgtcat acgagcgcgt accaatgtcc acgcgcatgg
    25921 cgctgacgtc attcccctgc cggtcgccgt caagcaggcc tggaattttg cactcttcac
    25981 gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgcacgttg ccaaccggca
    26041 gcacatcaat tgccccttcg cgggagatgc tgcgcaccat aaatcccact tcgtccatat
    26101 gggcgcagat catcaccttc ggccctgtcg attcattgag gcggatcagc accgatccca
    26161 gaccatcaaa tcgcacttct ttttgcaggc gatccgcttc ttccagcagg atctgccgca
    26221 cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat agcgataaat
    26281 ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca cttctgcgcc
    26341 ttgcggggta accagcacaa catcttcgat gcgcacgccc ccttgccctg gcaaataaat
    26401 ccccggctcc acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc gcggtgaaaa
    26461 acgcggatct tcatggactt caatgccgat agcgtgacca gtgttatgac cgaaatagtc
    26521 gccaaatcca gcctcggtaa tgacccggcg cgcggcgtcg tcaatctgct ggcagcgcac
    26581 gccggggcga attgcagaga ttgccgcgag ctgtgcctgc aggacaatct gatagacgtc
    26641 aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg tgcgcgtcat
    26701 atcagagcag tagccctgat acagcgcacc aaaatcgaga gtgacaaact cgcccgctgc
    26761 aacaatcttg tcgctggctt tgccgtgcgg cagcaccccg cgccagccgc tggcgacaat
    26821 ggtgtcaaaa gaggcttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc
    26881 tatctcgcgc tcgctcatcc ccgcctgaat aaagcggcga atatgctccg ctccgcgatc
    26941 ggcaatccca caggcgaggc ggattatctc cacctcctct ggcgttttga tttgccgcag
    27001 cacatccggc gtggcgctaa ccagtttcgc attgagttca gacttccagc ggtgcgcggt
    27061 ttcccaactc acctgctggc cttcaaaacc gagcgtttgc aactgctcat cggcaatgat
    27121 ttgattgacg atagtggtaa gcgtgttcgt cgcgtcaagc aaatgcagct ggtagccttg
    27181 cgcacgggct tccacctcaa cgaaatagct cgaatctacc agaatgtgcg cactttcacg
    27241 gctaataacc acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg
    27301 tgaggagaga agcactgcat ccagttgttg ctccttaagc cagtcgcgca gcgaagcgag
    27361 taatgtcatc tggattttcc ttcttacagg ctatcgatta acaatttgcc tttacggaac
    27421 atcatcagac gcaggaacac caccatcagc gcagttatga ctgcccccag cgcgatcccc
    27481 gccatataga cgccaaggtt agtgaccagc ggccatgccc agatagcgga ttccgggaac
    27541 cattgcactg cgcccagcca gacagcggcg gttgagccga caatcgcgcc gaccatatac
    27601 gacggaatgg cggtgatggg gctttccagc gcaaacggaa tcgcaccctc actaatcccc
    27661 ataaaggcaa ggaacatcgc ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt
    27721 ttgccggtta aacgacggtc aataatggtc gccagcccca gaccaatcgg aggaataacg
    27781 atagcgatag aacgagcggt gaccggtaat acgtggtcag tggtaaagct gaaggcaacg
    27841 aaacctgcgg ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc
    27901 attgcgtaca tcagcgcgcc cttctcacct gcggcagtca gcacggtacg gataacgccg
    27961 ttgatccagc cgccaaaggg ggtaatgacg tagtacatcg ccagcatgac aaaaatggcg
    28021 gaaagaatcg gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt
    28081 ttctggttca tccacttcac aaggtaaccc gcgacgatag aaataatcag cgcaccaatg
    28141 aaggtggacg gcaccggcga agaggtcgcc cattgcatag tgctgggatc gaagttcagc
    28201 agttgcgtcg gctgggtaga catcaagccg ccgataaaac cagccgggaa cgccagtttg
    28261 ccaccgatag agttcgccac aaaagcagca aacatcggaa tggcaaaacc aaacaacacg
    28321 ccgccgaagg actgcgacag ccaggcaaat ttcagtaagg agaggtcgaa accggagaat
    28381 ttcccgctat taagagcatc catgatgccg atctcagcag gaattttaag ccagctataa
    28441 gcaatcagct ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt
    28501 gaaatccctg acatcacatg ctgcgggagt tcaccccaga agctgctttt agaggcctgc
    28561 ggattcttaa ctgccgctgc cgcaccggat gcgccaggca caacggttgc actgcgtttt
    28621 ttaatggcca taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct
    28681 tcgatttctt tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa
    28741 acgtcgcgtg attcgaaacg ctcattatct tccggggtaa ctgccacgga gtggatgatg
    28801 atggttgctt cggcgatatc ctgcgccgtc aggcgattct ggataccgtc cgcgccctgg
    28861 gtttcaattt tcacttcata accggcttct accgccgctt cttccaacgc ctgagcggcc
    28921 ataaaggtgt gagccaggcc cattgggcag gcacataagg caatcagttt cttactcatc
    28981 gttaattcct cattcagtga tagttgacaa gtgaaattat gaacgtgcaa aacaaatcgc
    29041 cgttaccaga caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg
    29101 caggtcggcc ttgtgagggg acacaaagaa aaattgaatt aactgtatga tttaaacgat
    29161 tatcgggaga gttacctccc gatataacag gaaggattta cagaatgtgc cctaaggtct
    29221 ggcgtaaatg tgcaccggaa ccgagaagac ccggattgtc atggacgatg agatacaccg
    29281 gaatatcatg gacatattct ttaaagcgcc ctttatcttc aaatgcagca cggaaaccag
    29341 aggctttgaa gaactcaagg aagcgcggca cgataccgcc cgcaataaac acgccgccaa
    29401 atgtcccgag attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg
    29461 acaacgcgcg gcggcaatcg gtacagctgt cagccagcgc gcgttcggta atatcttttg
    29521 gcttgagatt ttctggcagg cggttgtcag ctttcacaat tgcgcgatac aaattcacca
    29581 gcccagggcc agaaagcacg cgctccgccg aaacatgacc aatttccgca cgcaatattt
    29641 cgaggataat ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg
    29701 gcaagcttac ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cctgttccgg
    29761 caccgtaaac cgcaataggt ttaccttcta ccggttctgc gccaccaaac tgaatcagat
    29821 gctctttttt cagcatcggg atcgccatcg atacagcggt aaaatcgtta ataatttcca
    29881 gatggctaaa accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg
    29941 tcattgccac ccagtcaccg gtaattgggc aagcgatggc aatacagccg tctttcacct
    30001 cgaccttatg ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc
    30061 ctgaataggt cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg
    30121 cgttggtgcc gcccacatca ccgactaatg catactttat cattcttcaa ctgctccgct
    30181 aaagtcaaaa taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga
    30241 aaggcaggct ccctgtaaat atcgatctgg gtcacacatt tactttatcg tttcagcacc
    30301 aattgcagcg atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa
    30361 tagaacatgc tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca
    30421 attgggattg cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag
    30481 aatttgctct ggcaacgact gccgggaact ctggggatag cccaggattc acctctctgg
    30541 atcatcggtg tattaacgct aacgggtatt gcggtggggt tggttatccg tttcagccag
    30601 ggtcatgccg gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccagt tccgccctct
    30661 gcgctaccag gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg
    30721 ccggaacatc cgatcatgac catcaatatc gcccttgccg tcgccattgg cgctcgtctg
    30781 ttaccgcgcg tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg
    30841 ctgtttggca cacctgttgc agcggcgttg atattttcgc aaaccttaaa tggcagtagt
    30901 gaagttccgc tatgggatcg tctctttgcg ccgttaatgg cggcagcagc tggtgcacta
    30961 actaccggat tatttttcca tccccatttt tcactgccca ttgcgcatta cggacagatg
    31021 gaaatgaccg atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg
    31081 gtcgccgtat ggtgcttacc acggttgcac gcgatgatga atcaaatgaa aaatccggtg
    31141 ctcgtgctgg gtattggcgg atttattctc ggtattctgg gggttattgg tggaccagtt
    31201 tcgctgttta aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc
    31261 agcgattact ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt
    31321 ggctttcgcg gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg
    31381 ctgcatgagc acgtacccgc cgtaccagcg gcaataaccg tttcttgcgc tattctcggc
    31441 atcgtgctgg tggtaacacg cgatggctgg ttaagtcttt ttatggcggc ggtcgttgta
    31501 cccaatacca cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta
    31561 gcaggtaagc cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataacgtt
    31621 tagcaaaaaa tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct
    31681 ccccgtaata ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg
    31741 ttcaggtcac tgtttctggc ggccgccctg atggcattta ccccgcttgc agcaaacgca
    31801 ggtgaaatca ccctactgcc atcaatcaaa ttacaaattg gcgatcgcga tcattacggt
    31861 aattactggg acggtggtca ctggcgcgac cgtgactact ggcatcgcaa ttatgagtgg
    31921 cgcaaaaacc gttggtggcg tcatgataat ggctatcatc gtggctggga taagcgtaaa
    31981 gcgtatgagc gtagctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc
    32041 cgcggacatg gacaccgcca ttaaacacat tcgccaatgg agcacaatgc ctgatgcgac
    32101 gctagcgcgt cttatcaggc ctacaaaccg gatctttaac gcgtagggcg gataaggcgt
    32161 ttacgccgca tccgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc
    32221 gtccccacca acaaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc
    32281 tgttttacgc gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc
    32341 agcagtggaa ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga
    32401 atccgtgtcg gatctaatcc catcagaatg acaataaatg acggcagcat ggtgactgta
    32461 cgacgcaccc acagcgggat atggaagcga atgaacccct gcatcaccac ctgccctgcc
    32521 agtgtcccca ctaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt
    32581 gccgcagcat ggcttaacag cggttgcagc gtcagataag cctcatcaag atcggcaaca
    32641 ccagtatgac cagaaaagtg gaacgccgcc gcagctgtag ccatcatcgc cagattgaca
    32701 aaaccggcaa tagtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga
    32761 cgcgaaccgc catgtaaatg ctgagtgagc gaggagtgca aataaatcac atgcggcata
    32821 atcgtcgccc ctaacacgcc tgctgccagg aagaccgctt ccgaagtagg taaactcggg
    32881 agcaccattc ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg
    32941 taagccgcgg caacaaacaa cagtaacccg ccaatcactt tctccagcgt tttttgcccg
    33001 cgacgttgca gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac
    33061 aacgaaacac caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg
    33121 gttgccatcg caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag
    33181 tgatcgcgaa tctgctccgc cagattttta ccggtggcaa tccctagttt ggcagagagg
    33241 atctgaatca gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg
    33301 aagctggcac ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc
    33361 gcaatgaacg caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta
    33421 ctctcaacgc gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat
    33481 gctatgataa acgagaatga ttatcaaatt catttaaatg gattgtggtg atttctgtga
    33541 tagaccagga ttatgactac gaaaagattg atgacttgaa tgttatggtt atgtttaatg
    33601 ttagcacatt tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagat
    33661 cactattttt gaagcctgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct
    33721 taaccatgtt acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat
    33781 ggaccgcgtc cttcattttg tactggcact tgccgttgtt gcgattctcg cactgctggt
    33841 aagcagcgac cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt
    33901 gttactggcg tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc
    33961 cgaaatgttc gaaaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag
    34021 catgaatgat caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat
    34081 ctctgcactg atcggtattc tccagcacat tcgcgtgttg ccggtgatca tccgcgcaat
    34141 tggtttcctg ctctccaaag tcaacggcat gggcaaactg gaatccttta acgccgtcag
    34201 ctccctgatt ctgggtcagt ctgaaaactt tattgcctat aaagatatcc tcggcaaaat
    34261 ctcccgtaat cgtatgtaca ccatggctgc cacggcaatg tccaccgtgt cgatgtccat
    34321 cgttggtgca tacatgacca tgctggaacc gaaatacgtc gttgctgcgc tggtattgaa
    34381 catgttcagc acctttatcg tgctgtcgct gatcaaccct taccgtgttg atgccagtga
    34441 agaaaacatt cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga
    34501 atacattctg gcaggtttca aagttgccat tatcgttgcc gcgatgctga tcggctttat
    34561 cgccctgatc gctgcgctga acgcactgtt tgccaccgtg actggctggt ttggctacag
    34621 catctccttc cagggcattc tgggctacat cttctatccg attgcatggg tgatgggtgt
    34681 tccttccagt gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga
    34741 gttcgttgcg atgatggatc tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg
    34801 catcatctct gtgttcctgg tttccttcgc taacttctct tcaatcggga ttatcgcagg
    34861 tgcagttaaa ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaaact
    34921 ggtttacggc tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta
    34981 agaccataca taaaaaagcc ggggataatt ccc
//
LOCUS       DAJTNN010000021        34857 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTNN010000021 DAJTNN010000000
VERSION     DAJTNN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.021"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(146..1405)
                     /locus_tag="OO150_000919"
     CDS             complement(146..1405)
                     /locus_tag="OO150_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5515148.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(1596..2459)
                     /locus_tag="OO150_000920"
     CDS             complement(1596..2459)
                     /locus_tag="OO150_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5515149.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            2586..3302
                     /gene="rph"
                     /locus_tag="OO150_000921"
     CDS             2586..3302
                     /gene="rph"
                     /locus_tag="OO150_000921"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5515150.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            3368..4009
                     /gene="pyrE"
                     /locus_tag="OO150_000922"
     CDS             3368..4009
                     /gene="pyrE"
                     /locus_tag="OO150_000922"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5515151.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(4046..4642)
                     /gene="slmA"
                     /locus_tag="OO150_000923"
     CDS             complement(4046..4642)
                     /gene="slmA"
                     /locus_tag="OO150_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5515152.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(4749..5207)
                     /gene="dut"
                     /locus_tag="OO150_000924"
     CDS             complement(4749..5207)
                     /gene="dut"
                     /locus_tag="OO150_000924"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5515153.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(5185..6405)
                     /gene="coaBC"
                     /locus_tag="OO150_000925"
     CDS             complement(5185..6405)
                     /gene="coaBC"
                     /locus_tag="OO150_000925"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5515154.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSENNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            6577..7245
                     /gene="radC"
                     /locus_tag="OO150_000926"
     CDS             6577..7245
                     /gene="radC"
                     /locus_tag="OO150_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5515155.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            7462..7698
                     /gene="rpmB"
                     /locus_tag="OO150_000927"
     CDS             7462..7698
                     /gene="rpmB"
                     /locus_tag="OO150_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5515156.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARSEKY"
     gene            7719..7886
                     /gene="rpmG"
                     /locus_tag="OO150_000928"
     CDS             7719..7886
                     /gene="rpmG"
                     /locus_tag="OO150_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5515157.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            7984..8793
                     /gene="mutM"
                     /locus_tag="OO150_000929"
     CDS             7984..8793
                     /gene="mutM"
                     /locus_tag="OO150_000929"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5515158.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(8832..9311)
                     /gene="coaD"
                     /locus_tag="OO150_000930"
     CDS             complement(8832..9311)
                     /gene="coaD"
                     /locus_tag="OO150_000930"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5515159.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(9319..10596)
                     /gene="waaA"
                     /locus_tag="OO150_000931"
     CDS             complement(9319..10596)
                     /gene="waaA"
                     /locus_tag="OO150_000931"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5515160.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            11009..12067
                     /gene="rfaQ"
                     /locus_tag="OO150_000932"
     CDS             11009..12067
                     /gene="rfaQ"
                     /locus_tag="OO150_000932"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5515161.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLRVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            12064..13188
                     /locus_tag="OO150_000933"
     CDS             12064..13188
                     /locus_tag="OO150_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5515162.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            13181..13978
                     /gene="rfaP"
                     /locus_tag="OO150_000934"
     CDS             13181..13978
                     /gene="rfaP"
                     /locus_tag="OO150_000934"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5515163.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            13994..15010
                     /gene="waaO"
                     /locus_tag="OO150_000935"
                     /gene_synonym="rfaI"
     CDS             13994..15010
                     /gene="waaO"
                     /locus_tag="OO150_000935"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5515164.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            15027..16022
                     /locus_tag="OO150_000936"
     CDS             15027..16022
                     /locus_tag="OO150_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR5515165.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            16032..16724
                     /gene="rfaY"
                     /locus_tag="OO150_000937"
     CDS             16032..16724
                     /gene="rfaY"
                     /locus_tag="OO150_000937"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5515166.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            16750..17775
                     /locus_tag="OO150_000938"
     CDS             16750..17775
                     /locus_tag="OO150_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR5515167.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            17860..18843
                     /locus_tag="OO150_000939"
     CDS             17860..18843
                     /locus_tag="OO150_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5515168.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIVNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRDCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            18889..20142
                     /locus_tag="OO150_000940"
     CDS             18889..20142
                     /locus_tag="OO150_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5515169.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(20212..21183)
                     /gene="rfaC"
                     /locus_tag="OO150_000941"
     CDS             complement(20212..21183)
                     /gene="rfaC"
                     /locus_tag="OO150_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5515170.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(21187..22233)
                     /gene="rfaF"
                     /locus_tag="OO150_000942"
     CDS             complement(21187..22233)
                     /gene="rfaF"
                     /locus_tag="OO150_000942"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5515171.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(22243..23175)
                     /gene="rfaD"
                     /locus_tag="OO150_000943"
     CDS             complement(22243..23175)
                     /gene="rfaD"
                     /locus_tag="OO150_000943"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5515172.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            23389..24585
                     /gene="kbl"
                     /locus_tag="OO150_000944"
     CDS             23389..24585
                     /gene="kbl"
                     /locus_tag="OO150_000944"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5515173.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            24595..25620
                     /gene="tdh"
                     /locus_tag="OO150_000945"
     CDS             24595..25620
                     /gene="tdh"
                     /locus_tag="OO150_000945"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5515174.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            25859..26893
                     /gene="waaH"
                     /locus_tag="OO150_000946"
                     /pseudo
     CDS             25859..26893
                     /gene="waaH"
                     /locus_tag="OO150_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(26880..27839)
                     /locus_tag="OO150_000947"
     CDS             complement(26880..27839)
                     /locus_tag="OO150_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5515175.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(27843..29126)
                     /gene="envC"
                     /locus_tag="OO150_000948"
     CDS             complement(27843..29126)
                     /gene="envC"
                     /locus_tag="OO150_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5515176.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(29136..30680)
                     /gene="gpmM"
                     /locus_tag="OO150_000949"
                     /gene_synonym="pgmI"
     CDS             complement(29136..30680)
                     /gene="gpmM"
                     /locus_tag="OO150_000949"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5515177.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            30925..31356
                     /locus_tag="OO150_000950"
     CDS             30925..31356
                     /locus_tag="OO150_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5515178.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            31498..31749
                     /gene="grxC"
                     /locus_tag="OO150_000951"
     CDS             31498..31749
                     /gene="grxC"
                     /locus_tag="OO150_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5515179.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            31812..32279
                     /gene="secB"
                     /locus_tag="OO150_000952"
     CDS             31812..32279
                     /gene="secB"
                     /locus_tag="OO150_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5515180.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            32279..33298
                     /gene="gpsA"
                     /locus_tag="OO150_000953"
     CDS             32279..33298
                     /gene="gpsA"
                     /locus_tag="OO150_000953"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5515181.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            33378..34199
                     /gene="cysE"
                     /locus_tag="OO150_000954"
     CDS             33378..34199
                     /gene="cysE"
                     /locus_tag="OO150_000954"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5515182.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(34252..34725)
                     /gene="trmL"
                     /locus_tag="OO150_000955"
     CDS             complement(34252..34725)
                     /gene="trmL"
                     /locus_tag="OO150_000955"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5515183.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         8715 a         8272 c         8701 g         9169 t
ORIGIN      
        1 tcggctgctc tgttaagcct gaaaaaaggg gcgggtggta attttttcat tgtgtcacct
       61 tttacgctgc gccttatttt gtgattgagc caggcggtaa ggcttcccgc ttttcgcccc
      121 gtcgagctag gctcaatcta ttcagttagt tcgcccttct gagaggcgtt acgttgtagt
      181 cttttccgct ttccagtgcc accagcagat cagcccactg tgccagtgct gctttacgct
      241 catcaaaata ctgatggcgg ttataaatcc cctctacgcc cttaatgcga tgattgaggc
      301 atcgttccgc cacaacagga tcaacaccca acgctgctaa atgagtacgt gcggtgcgtc
      361 gaaagtcgtg aatcgtgaaa ttaggcacat ccggcatttc ggcgcgaacc ttagccagtg
      421 ctacgggtaa agtgctttcc tgaatatgtg ggatcattct gttttgcatc ttcctggccg
      481 gaagcaccca tgcgctatta catgaaaagg tgtgtagctc tctcaaccat tcaacggctg
      541 gggaaggtag aggtatatca atagggtctc cgtttttgct gcgttcttcc ggcaaatgcc
      601 ataccgcacc atctaaatca aactcttccc atcgtgcggc gcataattcc attttgcgga
      661 cgcataacgc taacagtaat ttgaacgtga tttcgttctg gcgactgaat cccttagccg
      721 tgcgcatggc tttgaatagc tggattaact catcgcgggt taaccagcgg tcacgggcag
      781 cttctttccc tccggcgtct gccacctcaa aggctgaaca ggggttaatc tctagcgcgt
      841 gccgtttgat tccgtagtcg aatatgcggc gcgtccatct cagcacgtcc gttgctatgg
      901 tcggcgctcc acggtcaaca atacctttca gcatgtcatc gatatggcgc ggtttcacgt
      961 cctctacctt catgctgcca atgcaggggt ttatatcttt gtcgatacgg cggcggagta
     1021 tatcggggtg cttccagcgc gggaggatct gacgctcaaa gtattcagcg gcaagctctg
     1081 aaacgcgcat agcgttcttc tctgcttcca tcttcgccag tgcttcggtt ttgcgcttct
     1141 gcttctctcc tgcaacgtca tagcccagcg caacgcgagc cgatagctct ttggctgtct
     1201 ctctggcctt tgatagtgat agctcgctat acgaaccaat tagcatggcg cgggacttcc
     1261 ctgcgagttt atagcggaat ctccatgtgg gggttttgtc ggcttcacgg taacgtaggt
     1321 ataaaccgtt accgtctgcc cgcccctcga atcgctctcc gctcttaatc catgcgcgga
     1381 tctgcatgtc tgttagtttt ggcattttgc gaatatccaa actttgaata gggggtgtac
     1441 ccaaagcaat gtacccaaac ctcattgggt acacctttgt gtacaccata atcatgcgct
     1501 ttgttgcgtc tttatgatag cccatgataa caaaaaagcc cgctgggttg cgggcttggt
     1561 gtgatttatg cgtcgtccta aatgttgctg atagcttact cgatattctg aatctgctcg
     1621 cgcatctgct caatcaacac tttcagctca atcgcggagt ttgtcacttc ggcattgata
     1681 gacttcgacg caagagtgtt cgactcgcgg ttgaattcct gcatcataaa gtccagacga
     1741 cgaccaacgg cttctttttt cttcagaatg ttgtaggtct ctttgacatg cgcttccagg
     1801 cggtccagtt cttcggcaac gtcaattcgt tgtgccagca gaaccagctc ctgctccaga
     1861 cggttgtttt ccaactgaac ctgagcatct tccagcttcg cgaccagacg ctcacgctgc
     1921 cattgcagga tttccggcat atgggcgcgg actttgacca cttcggcggt gacgccttcc
     1981 agacgctgct cgatcaatgc tttcagtgcc tgaccttcgg tttcgcgcgc gacaataaag
     2041 tcgtccagcg taccatccag cgccgcgaga atttcagcgg caatggcgtc aagatcctgc
     2101 tcctgggctg ccatcacgcc cggccagcgt agaatatcaa ccgggttgat ttccccttcg
     2161 tcactctgca tttttaccca gttcgcggca gttaccagct gtttagccag tttttcgttg
     2221 aggatcagct caccttgcgc gctaatatct ggctcatagc gcagggtaca ttccacttta
     2281 ccgcgcgtca ggcgagaacg aatacgctcg cgaacgacag gttcaaggct acggaactgc
     2341 tccggcagac gaaagtaagt ttccagataa cgctggttta ccgagcgcat ttcccaggtt
     2401 gcgctccccc attcaccctt gatttcacgc cgggcgtagg cggtcatact gcggatcata
     2461 gacgttcctg tttataaaag gagaggtgga aggattatag ccatcgatgc cttgtcagga
     2521 taggaataac cgccggaagt ccgtataatg cgcagccaca tttgtttcaa gccggagatt
     2581 tcaatatgcg tccagcaggc cgtagcaata atcaggtgcg tcccgttacc ctgactcgta
     2641 actatacaaa acatgcagaa ggctcggtgc tggtcgaatt tggcgatacc aaagtgttgt
     2701 gtaccgcctc tattgaagaa ggcgtgccgc gcttcctgaa aggtcagggc cagggctgga
     2761 tcaccgcaga gtacggcatg ctgccacgtt ctacccacac ccgtaacgct cgtgaagcgg
     2821 cgaaaggtaa gcagggtgga cgcacaatgg aaatccagcg tctgatcgcc cgtgctcttc
     2881 gcgcggcagt agatttgaaa gcgctgggtg agttcaccat tacgctggac tgcgacgtgc
     2941 ttcaggctga tggtggcacg cgtaccgcgt cgattacggg tgcctgcgtg gcgctggcag
     3001 atgcgctaca gaagctggtg gaaaacggca agctgaaaac caacccgatg aaagggatgg
     3061 tagccgcagt ttctgtcgga attgtgaacg gcgaagcggt ttgcgatctg gaatacgttg
     3121 aagactctgc cgcagagacc gacatgaacg tagtgatgac cgaagacggg cgcatcattg
     3181 aagtgcaggg gacggcagaa ggcgagccgt tcacccatga agagctactc accttgttgg
     3241 ctctggcccg agggggaatc gaatccattg tagcgacgca gaaggcggcg ctggcaaact
     3301 gatttttaag gcgactgatg agtcgccttt tttttgtctg tagaaaagta agatgaggag
     3361 cgaaggcatg aaaccatatc agcgccagtt tattgaattt gcgcttggca agcaggtgtt
     3421 aaagtttggc gagtttacgt tgaagtccgg gcgcaaaagc ccctatttct tcaacgccgg
     3481 gctgtttaat accgggcgcg atctggcact gttaggccgt ttttacgctg aagcgttggt
     3541 ggattccggc cttgagttcg atctgctgtt tggccctgct tacaaaggga tcccgattgc
     3601 caccactacc gccgtggcgc tggcggagca tcatgacctt gacctgccgt actgctttaa
     3661 ccgcaaagag gcaaaagacc acggtgaagg cggcaatctg gttggtagcg cgttacaagg
     3721 acgcgtaatg ctggtagatg atgtgatcac cgccggaacg gcgattcgcg aatcgatgga
     3781 gattattcag gctaatggcg cgacgcttgc tggcgtgttg atttcgcttg atcgtcagga
     3841 acgggggcgc ggcgagattt cggcgattca ggaagttgag cgtgattaca actgcaaagt
     3901 gatctctatc atcaccctga aagacctgat tgcttacctg gaagagaagc cggaaatggc
     3961 ggaacatctg gcggcggtta aggcctatcg cgaagagttt ggcgtttaaa gaaactcgcc
     4021 ggatgaaaag tcatccggcg tcatattact gcaactgtgc cgcaattagc ggccagcggg
     4081 cgtcaaaatc atccgtcggg cggtatttaa attcgctgcg gacaaaacgt gacagcatac
     4141 cttcacagaa ggccaggatc tggcttgcca gcagggtttc atcggtggtg taaccttcac
     4201 cctcacgcat tctcttttca cgcaatacct gacgcagctg cgcttcaata cgctcgaaca
     4261 gctggttgat gcgcccttgc aggcgatcct gttcaaacat tagcgcatga ccagtgagga
     4321 tgcgggtcag gccaggatta cgctcaccaa aaccgagaag cagcaacaca atcagacgca
     4381 ggcgcgctgt ggtgtctttc tcatctttca gaatcaggtt gatgcgagta atcaggctat
     4441 cttcgataaa ctcaatcagg ctatcgaaca tgcgggtctt actggggaag tggcgataca
     4501 gtgccgcttc ggaaacgccg acagaggcgg ccagttttgc cgtcgtgata cgttggcttc
     4561 catcgctgga ttccagcatc agcgccagag actgaagtat ttcctcgcga cggttccttt
     4621 tcgcagtttg tttttctgcc atgttacaaa atacccctga aaataagcac ttgccaggcg
     4681 gcacccacgc tatgaccgca aacgaaatgt ttgcggctat gttatgacgt tattcggatg
     4741 cgtatgtgtt actgacgacc agagtgacca aagccgcctt caccgcggtc ggtggcgtcg
     4801 aaatcttcca ccagattaaa ttcagcctgt actaccggaa caaaaatcat ctgggcgatg
     4861 cgttcgccag gttgaatggt gaagctgtcc tgaccacggt tccacacgga aatcatcaac
     4921 tggccctgat agtcggaatc gatcaatcct accaggttac caagcacgat accgtgctta
     4981 tgtcccaatc cggagcgcgg cagcatcatt gccgccagtg aaggatcggc aatatgaatc
     5041 gccagcccgg tcggaaccag cgtagtgtca cccggagcca gttctacggc gtcgtcgaga
     5101 caggcacgca ggtcaagtcc ggcagagcca gaggtggcat aagtcgggag cggaaattcc
     5161 ttcccaacgc gcgggtccag aatcttaacg tcgatttttt tcatcataac gggtcacgat
     5221 ctcgtcgagt aatagttggc caaggagctc tttgcgctca agcggtaaga ctttatctcc
     5281 gtcctgccag aaaaggtgta atgcgttgtt ttcgctgtta aatccttgag ttggctggga
     5341 aacatcgttc gcgcagatca gatcaaggtt tttacggata cgtttttgcc gggcgtattc
     5401 ttccacatta tttgtttcgg cggcaaatcc aacgacgtag ggtcgatggt cttttagtgc
     5461 ggcaacgcct gcgacgatat cggggttttt aaccattttt attgttaatt catcaccctg
     5521 cgtggcctgc tttttgattt tttctggggc cacggtagct gcgcgataat ccgccacggc
     5581 ggcgcagccg ataaaaatat tttgctgctg tacagaagca ttcacggcgg cttccatttc
     5641 cagcgcggtc atcacatcaa cacgtttaac aaacggtggc gtcggtagtg aaaccggacc
     5701 tgataccagc gtgacgttcg cgccacgacg ggcagcggcg gcggcgatag caaaacccat
     5761 cttgccggag ctgtgattag agatataacg cacgggatcg agcggttcac gcgtcggacc
     5821 ggcggtaatc ataatgttca gatgtttcag gtcgttgacg ggcgaaaaat gcgccaccgc
     5881 catatccaca atggttaacg gatcgagcat tcgcccagga ccgatatcac cacaagcctg
     5941 actgccactg tctggccccc agatgagcaa accacgggaa gcaagcccct ctaaattatg
     6001 ctgcgtggca gcggcacggt acatctgctg gttcatggcg gggagcacgg ctacaggcgc
     6061 aggtgtagcc agacaaatcg tcgataccag gtcattcgcc attccggcag caacacgggc
     6121 aatcaaatct gccgtggcag gggcgagaat cactaaatca gcccatttac ccagctcaat
     6181 atggcccata gcggcttcgg ctgccgggtc cagcaaactg tcggaaacgg gataaccaga
     6241 aaccgcctgc aagctaagtg gggtgataaa ggcttttgcc gcttcggtca tggctacgcg
     6301 gacgtcggcc ccgcgatcgc gcaaacgacg caccagttca ggggttttat aggcagcaat
     6361 accgccgcta acgccgagaa cgattttttt accggccagg ctcatgatga tttttcctgt
     6421 tgggtgacac cagaagttgg cgattttatc acaatacttt tgttgtcgtg ccttcacctg
     6481 agaatcactt tgcgaggcgc tttccaggat tgaaaactgg ccgtcgattt aacggaacgg
     6541 ctatgacagg atgcgagcac cacaaaggag gtgaaggtga aaaacaattc acagctgttg
     6601 atgccgcgcg aaaaaatgct gaagtttggt attagcgcct taacggatgt cgagctgctg
     6661 gcgctatttc tgcgtaccgg aacgcgcggt aaagatgtat taaccctggc aaaagagatg
     6721 ctggagaatt tcggctctct ttatggcttg ttaacctctg aatatgaaca atttagtggc
     6781 gttcatggaa ttggcgtggc gaaatttgcc cagttaaagg ggattgctga actggcgcgg
     6841 cgttactaca acgtgcggat gcgtgaagaa agccctttac tcagcccgga gatgacgcgg
     6901 gaatttttac aaagccagct tacgggtgag gagcgggaga tctttatggt gatctttctc
     6961 gactcacaac accgggttat aacgcatagc cgtctttttt ccggcacgct aaaccatgtt
     7021 gaagtccatc ctcgggaaat tatccgcgaa gcgataaaaa taaacgcctc ggcgctgatc
     7081 cttgcacata atcacccttc gggttgtgct gaacccagta aagcggataa actcattact
     7141 gaacggataa taaagagttg tcagttcatg gatttacgcg tgctcgacca tatcgtgatt
     7201 gggcgtggag agtatgtttc ttttgccgaa cgcggctgga tttaacccgc tatgcgcgat
     7261 ccttcgggat ctttgtctgt tcgggacttg agcacatcgc tgagtcagcg tatactacgc
     7321 cacctttgag aatctcgggt ttggcatttg ggcctggcaa tcgagagttc acatagaact
     7381 gcgatgactg ggctgtaaag cctgacgagg cgccaatacc ccatacgaag ctcgagctaa
     7441 tttgattttt ggagaataga catgtcccga gtctgccaag ttactggcaa gcgtccggtg
     7501 accggtaaca accgttccca cgcactgaac gcgactaaac gccgtttcct gccgaacctg
     7561 cactctcacc gtttctgggt tgagagcgag aagcgttttg tcaccctgcg cgtatctgct
     7621 aaaggtatgc gtgtaatcga taaaaaaggc atcgatacag ttctggctga actgcgtgcc
     7681 cgtagcgaaa agtactaagt acttagagga aataaatcat ggctaaaggt attcgtgaga
     7741 aaatcaagct ggtttcttct gctggtactg gtcacttcta taccactacg aagaacaaac
     7801 gtactaagcc ggaaaaactg gaactgaaaa aattcgatcc agttgttcgc cagcacgtga
     7861 tctacaaaga agcgaaaatc aaataattct cgctttgatg taacaaaaaa cctcgctccg
     7921 gcggggtttt ttgttatctg cttgccccca tattgactgc atctgttcat tcctggagat
     7981 gctatgcctg aattacccga agttgaaacc agccgccgcg gcatagaacc gcatctcgtt
     8041 ggtgcaacca ttcttcatgc agtggtgcgc aacggacgct tgcgctggcc ggtttcagaa
     8101 gagatctacc gtttaagcga ccaaccagtg cttagcgtgc agcggcgggc taaatatctg
     8161 ctgctggagc tgcctgaggg ctggattatc attcatttag ggatgtctgg cagcctgcgc
     8221 atccttccag aagaacttcc ccctgaaaag catgaccatg tggatttggt gatgagcaac
     8281 ggcaaagtgc tgcgctacac cgatccgcgc cgctttggtg cctggctgtg gaccaaagag
     8341 ctggaagggc ataatgtgct ggcccatctt ggaccggagc cgcttagcga cgatttcaat
     8401 ggtgagtatc tgcatcagaa gtgcgcgaag aaaaaaacgg cgattaaacc gtggctgatg
     8461 gataacaagc tggtggtagg ggtagggaat atctatgcca gcgaatcact gtttgcggcg
     8521 gggatccatc cggatcggct ggcgtcatca ctgtcgctgg cagagtgtga attgttagct
     8581 cgggtgatta aagcggtgtt gctgcgttcg attgagcagg gtggtacaac gctgaaagat
     8641 tttctgcaaa gtgatggtaa accgggctat ttcgctcagg aattgcaggt ttacgggcga
     8701 aaaggtgagc cgtgtcgggt gtgcggtacg ccgattgtgg cgactaaaca tgcgcagcgg
     8761 gcaacgtttt attgtcggca gtgccagaag taattcatgc gcgccggatc gcataccatc
     8821 cggcataaac gctacgctaa cttcgccatc agcgcctgat ggacattctc cggcaggaaa
     8881 tgggtgacat cgccctgatg gcgcgccacc tctttcacca acgatgaaga gataaacgac
     8941 cactctttcg acggcatcag aaacacactt tccagttccg gcattaagtg gcgattcata
     9001 tgcgccagct gcatttcata ttcaaaatcc gccaccgcac gtaggccacg aatcagcacc
     9061 gtagcgtgtt gattacgggc gaagttcgcc atcaaatcac taaacccaac tacttccacg
     9121 ttccccagat gcgcggttgc ctgctgtgcc agcgccacac gctcttccag ggtaaacatc
     9181 ggttttttac tggggctggc ggcaatcgcc agaataacgt gatcgaacat ttgcgtggcg
     9241 cgcgtcacga tatcgatatg accattggta atgggatcga aagtacccgg ataaatcgcc
     9301 cgtttttgca taacaacctc aatgcgtttt cggtggcagg taaggttcca gcagttgaag
     9361 caggcgctgt agcgcgccct ggttttgata cagtacttca acggcatgac ggccatagaa
     9421 actacggtaa tcggcgtcgg tgagtaaaga ggaaacctct tttgcaagcg tagtggcatc
     9481 ggtaacggta atcagcccgc ttgcctgctc cagccgcgcg caaatgtctt taaaattaaa
     9541 agtatgcggc cccatcaata ccggaatagc gtgtgcggca gcttccagcg gattatgccc
     9601 accacgttca accagtgaac cgccaacaaa ggcgagatcg gcaatgccgt acagtaacat
     9661 caactcgccc atcgtatcgc caaccacaac ctgcgtgctg gtggagggga cttcccctga
     9721 agagcgtgtg atatagctta gtccagcctg gcggacaagg ttaatcgcat ccgggaagcg
     9781 ttctggatga cggggtacca ggatgagcaa taaattcggg aattgctgta acaatacctg
     9841 atgtgcggcg atcaccacac tctcttcacc ttcgtgagtg ctggtggcaa tccataccgg
     9901 gcggtgtggt gcccactggc ggcgcagcgt cacggcttta gcagccaact gcggcgttac
     9961 agaaatatcg aatttcaggc taccggtaac ggttacctgg ttattttttg cgcccagcgc
    10021 cacaaaacgt gcaccatctt cttcattttg ggcggcaatc agcgtaatac gacgcagcaa
    10081 gcgacggacg aatttaccca gtttggcata acctgcggcc gagcgggcag agagtcgcgc
    10141 gttcgcgatc accagcggaa ttttacgttt atgtagcgcc gcaatcaggt taggccatag
    10201 ttcggtttcc ataatcaaca ccagtttagg gtcgacttta ttcaggaaac gattaagtgc
    10261 atcgggcagg tcatacggca gataaacgtg ctgaacatcc ttcccgaaag ccgattgtac
    10321 gcgctccgaa ccggttggcg tcatggttgt aacggtaatc ggtaaatcag gataacgatg
    10381 acgcagcgcg cgcaccaacg ggatcgccgc cagagtttca ccgacggaga cggagtgcag
    10441 cataatgccg cctggtttta gcggatggcg gtaaaaaccg taacgttcac cccagcgttt
    10501 tcgataggcc ggagccttac gtccgcgcac ccagagccgt atccagatca gcggctgaat
    10561 aaggtagaga agggcggtgt aaagcaattc gagcatagta aatagctgac ttatggatgt
    10621 gctggggatt ctatgtattt agctgtggct ttaccattac ttttcccgtt tttgacttaa
    10681 atagcaattt acccgccaat tcttgctgaa catctatact ttttttgtat ttttatgcga
    10741 ttcattgatt tctgctgtac gtgcaggaag gaatcgcaga gcaacagtga aagaatatgg
    10801 ttgctaatga ttttatttga ttgatttctt tattgaaaat caacaatatg cttaaaaatc
    10861 attcgggtcg gattgccgcg aaagaagtga tacactagcg cgtcaatatc agtgcggtat
    10921 cagtattcag gtagctgttg agcctggggc ggtagcgtgc tttttctgct tcaccttaat
    10981 cggataatct caacaaaaag agttacttgt ggataagcta tttcgaagaa ttttgctcat
    11041 taagatgcgt tttcatgggg atatgttatt aactactccc gtcattagtt cgctgaaaaa
    11101 aaattaccct gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc cgatcctgtc
    11161 tgaaaatcca gagattaacg cgctctacgg cataaaaaat aaaaaagcaa aagcctcaga
    11221 aaaaattgcc aacttttttc atctcatcaa ggtattacgt gccaataagt atgaccttat
    11281 cgtcaatctc accgatcaat ggatggttgc tatactggtt cgcttattaa atgcccgtgt
    11341 gaaaatttcc caggattatc atcatcggca gtctgctttt tggcgtaaaa gtttcaccca
    11401 tttggtgccg ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc tgaccccatt
    11461 gcgagttgat tcgttggtga agcagacaac catgagttac ccgcctgcaa gctggaaacg
    11521 tatgcgtcgc gaacttgatc atgctggtgt tggacaaaat tatgtggtta tccaacctac
    11581 ggcgcggcaa atcttcaaat gctgggacaa cgccaagttt tccgctgtga ttgatgcctt
    11641 acatgctcgt ggttatgaag ttgttctgac gtccggccca gataaagacg atctggcctg
    11701 cgtcaatgaa attgcgcagg gatgccagac gccaccagta acggcgctgg ctggaaaggt
    11761 gaccttcccg gaacttggtg cgttaatcga tcatgcgcag ctgtttattg gcgttgattc
    11821 cgcaccggcg catattgccg ctgcagttaa tacgccgctg atatcgctgt ttggtgcgac
    11881 agaccatatt ttctggcgtc cctggtcaaa taacatgatt caattctggg cgggagatta
    11941 ccgggaaatg ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt cggttattcc
    12001 ggcggcagat gtcattgctg ctgtcgataa attactgccc tcctccacga caggtacgtc
    12061 gttatgatcg ttgctttttg tttatataaa tattttccct ttggcggttt gcagcgcgat
    12121 tttatgcgta ttgctcagac agtcgccgcc cgaggtcatc atgttcgggt ttatacccag
    12181 tcgtgggaag gcgaataccc tgatgtattt gaactgatca aagtgccggt taaatcgcat
    12241 accaatcacg ggcgcaatgc ggagtatttt gcctgggtgc aaaaacattt acgcgaacat
    12301 cccgtcgata aagtcgttgg attcaacaaa atgccggggc tggacgttta ttatgccgct
    12361 gatgtttgtt atgccgagaa agtcgcgcag gaaaaaggct ttttctatca cctgacgtca
    12421 cgttatcgcc attatgccgc ctttgagcgg gcaaccttcg aacagggcaa gccgacacag
    12481 ctgctgatgc tgacagataa gcaaatcgcc gatttccaga aacattatca gactgaagcg
    12541 gagcgttttc atattctgcc accggggatt tatcctgatc gtaaatatag ccagcagcca
    12601 gctaatagcc gtgaaatctt ccgtaagaag aatggaataa ccgaacaaca atatttattg
    12661 ttgcaggtcg gttcagactt cacgcgtaaa ggtgtcgatc gttccattga agcacttgct
    12721 tcgttaccgg attcgctgcg ccacaacaca ttgctatatg ttgttggaca ggataaaccg
    12781 cgaaaatttg aggcgctggc agaaaaacgc ggcgtgcgca gtaatgttca cttcttctcg
    12841 gggcgcaacg atgtctcgga attaatggcg gcggcggatt tattactgca tcctgcctac
    12901 caggaagcag cgggaattgt gctactggaa gcgattactg caggattacc ggtactaaca
    12961 acagccgttt gtggctatgc gcattatatt gtcgacgcca attgcggcga ggctattgct
    13021 gagccattcc gccaggaaac attgaatgag attttacgca aagcgttaac gcaatcttca
    13081 ttgcgccagg cctgggcgga aaatgcgcga cattatgctg atacacaaga tttatacagt
    13141 ctgccagaga aagcggcgga tatcataacg ggtggtctgg atggttgaac ttaaagagcc
    13201 gtttgccacg ttatggcgcg gtaaagatcc ttttgaggaa gttaaaacct tgcagggtga
    13261 ggtttttcgt gaactggaaa ctcgccgcac tctgcgattt gaaatggcgg gcaaaagcta
    13321 ttttctcaaa tggcatcgcg gcacgaccct gaaagagata atcaaaaatt tactctcatt
    13381 gcggatgcca gtattaggcg cagaccgcga atggagtgcg attcatcgac tgcgggatgt
    13441 cggcgttgat actatgtatg gggtggcatt tggcgaaaaa ggtattaatc cgctgagcag
    13501 aacctcgttt attattaccg aagatctgac cccaaccatc agtctggaag attactgtgc
    13561 tgactgggcg accaacccac cagatgttcg cgtaaagcgt atgcttatta agcgtgtcgc
    13621 gacgatggtg cgcgatatgc atgctgcggg cattaaccac cgtgactgtt atatctgtca
    13681 tttcctgctg cacttgcctt tttccggtaa ggaagaggag ttaaaaattt cggtaattga
    13741 cctacaccgg gcgcagcttc gcacgcgcgt tccacgtcgt tggcgcgata aagatcttat
    13801 agggctttat ttttcttcaa tgaatattgg cctgactcag cgggatatct ggcggtttat
    13861 gaaagtgtat tttgccgccc cgcttaaaga cattctcaag caggaacaag gactgctgtc
    13921 gcaagcagaa gcaaaagcca caaaaatcag ggaaagaacg attcgaaaat cgttgtaatt
    13981 tccggagaga aatatgagtg cccactattt taatccacaa gagatgatca ataagacaat
    14041 catcttcgat gaaaggccag cggcgtcagt ggcatcatca ttccatgttg cttatggcat
    14101 tgataaaaac tttctttttg gttgtggtgt ttcaatcacg tcagttttgt tacataacaa
    14161 cgacgtgagt tttgttttcc acgtttttat tgatgatatc cctgaagccg atatccagcg
    14221 tttagcccaa ttggcgaaaa gctatcgtac ctgtatccag atccatctag taaattgtga
    14281 acggcttaag gcattaccga cgaccaaaaa ttggtctatt gccatgtatt tccgttttgt
    14341 aattgcagat tactttattg atcaacaaga taagatcctt tacctggatg ctgatatcgc
    14401 ctgtcaggga aacttaaagc cgctgataac aatggatctt gccaataacg ttgctgctgt
    14461 tgttactgaa cgcgatgcta actggtggtc gttacggggt caaagtctgc agtgtaatga
    14521 acttgaaaag ggctacttta attcaggtgt cctgttaatt aatacactag cgtgggcgca
    14581 ggagtccgtt tctgctaaag cgatgtcgat gcttgctgat aaagccatcg tttcccgttt
    14641 cacctatatg gatcaagata tccttaatct tatcctgtta gggaaagtta aattcattga
    14701 tgctaaatac aatacgcaat ttagtttaaa ttatgaatta aaaaaatcat ttgtttgtcc
    14761 aattaatgat gaaaccgtat taattcatta tgtcggcccg acaaaaccct ggcattactg
    14821 ggccggttat ccaagtgcgc aaccttttat caaagccaaa gaagcatcgc cctggaaaaa
    14881 tgaaccgtta atgcggccag ttaactcaaa ctatgctcgt tattgcgcca agcataattt
    14941 taaacaaaat aaaccaatta acgggataat gaattatatt tattattttt atttaaagat
    15001 aataaaatga aattgagaaa agttaaatga atgaatttat aaaagaacgg ttttcgtatt
    15061 tagcagataa taaaaaagaa aacgccccag agctaaatgt ttcctacggt atcgataaga
    15121 attttttgta tggtgctggc gtttcaattt cttccgtttt gattaataat tcagatatta
    15181 attttgtctt tcatgttttc actgattatg tggatgatga ttatttaaag tcatttaatg
    15241 aaacagcaaa acaatttaat acctcaatta ttgtatattt aattgacccc aaatactttg
    15301 ctgatctgcc gacgtcacag ttttggtcat acgcgacata cttcagggta ttgtcttttg
    15361 aatatctgag tgaaagtatt tccacactgc tgtatctgga tgccgatgtt gtttgtaaag
    15421 gaagcctgaa acctctcaca gaaattatat ttaaagatga gtttgctgcg gtcattcctg
    15481 acaatgatag tactcaggcg gcatgtgcaa aacgcctcaa cattcccgaa atgaatggac
    15541 gttatttcaa tgcaggcgtt atctatgtca atcttaaaaa atggcatgaa gcaaatttga
    15601 caccgtattt actcaaactt ttacgagggg aaactaaata tggctctctt aaatatttag
    15661 atcaggatgc gttgaatatc gcatttaata tgaataatat ctaccttgcg aaggattttg
    15721 atactattta taccctgaaa aacgaacttt atgatcgtag tcatcgaaag tatcagcaaa
    15781 ccattaccga taaaacagtg ttgattcact atacagggat aactaaacca tggcatagct
    15841 gggctggata tccgtctgca tcatacttta atatcgcgcg tgaacaatct ccctggaaga
    15901 aatatcctct taaagaggcg cggactgttg cagaaatgca gaaacaatat aagcatctgt
    15961 ttgcccatgg tgagtatata aaaggcataa cttcattaat taagtacaag cttaagaaat
    16021 aaattactct tatgattaca agtatacgtt atcgcggctt ctcattttat tacaaagata
    16081 atgataataa atataaagaa atctttgatg aaatactggc ctataatttt aaaacagtaa
    16141 aagtattatg taatattgat gataccaaag tatcgttgat tgatacgaaa tatggtcgct
    16201 atgtttttaa agtctttgct ccgaaaacga aaaggaatga acgtttttta aaatcttttg
    16261 taaaaggtga ctactatcag aatttgatcg ttgaaaccga tcgtgtcaga agtgcaggtc
    16321 ttacttttcc taatgatttt tattttctgg ctgagcgtaa aattttcaac tatgccagtg
    16381 tttttatcat gcttatcgag tatgtcgagg gcgtagagct caatgatatg ccgattattc
    16441 cagaaaatgt taaagcagaa attaaggctt cgatggaaaa attgcatgcc ttaaatatgc
    16501 tatcgggcga cccacaccgg ggtaacttta tcgttagtaa agatggtgtc aggataattg
    16561 acctatccgg taaaagctgt acggcagagc gtaaagctcg tgatcgtctg gccatggaac
    16621 gtcacctggg tattgccaac gaaataaaag attatggcta ctattcagta atctacagaa
    16681 ccaaactacg caaatttatt aaaaaattaa aaggcaaagc gtaaaccaca cagtcaaaac
    16741 ggaaccaaca tggatttatt agcagagagt attactgaag tcgctgtctc tggggaaatt
    16801 gctaacaccg atcgtgtgtt aaatatcgct tacggtattg accgtaactt tttatttggt
    16861 gcggcagtat ctatgcaatc agttgttatg cataacccgg accttgcggt taagtttcat
    16921 ctctttaccg actacattga tgaagattat ctacaacgtg ttaatgcttt taccagcaaa
    16981 aatgctaacg ttgaagtaat aatttataaa gtctccaatg cctttattga tatcttcccc
    17041 agcctgaaac agtggtctta tgcaacattc ttccgtttag ttgcgttcca gtatttgagt
    17101 gaaactattg aaaatctgtt atatatcgat gctgatgtca tctgtaaagg ctcattagct
    17161 ggattgcttg atattaattt tgatgaggat aagttcgcag ctgttattaa agatgtgcct
    17221 tttatgcagg aaaaaccagc gaagcgtctg gctatagagg gacttccagg gaattatttc
    17281 aacgccggtg tagtatatct gcagcttgaa gcatgggcga aaaatgattt tatgaataaa
    17341 gccattgcta tgctggcaag tgacccgcag cacacgaagt ataaatgcct tgatcaggat
    17401 attttaaata ttctgttctt tggtcattgt aattttatta gcggcgatta tgattgcttt
    17461 tatggcattg actatgagtt aaaaaataaa agcgatgaag attataaaaa gaccattacc
    17521 gatgatacta agctgattca ttatgttggc gtaacgaagc cctggaacga ctggacgaat
    17581 tatccctgcc agaagtattt taatgaggct tatcaggctt cttgctggaa tgatgtggcg
    17641 tttattccag ccacgaatga aaagcagtat caagtgaaat atcaacatgc aaagaaaaat
    17701 ggtgatacgt ttaacgcttt tatttacttc attaaattta aattaaataa gtataaaaga
    17761 aaactatttg gataataata aaaatcatgg atgtctgagt tacaataata attaaggatg
    17821 agtactatca tattttatta gttcaggcaa gagaacacga tgagcaatga ttacccttta
    17881 gtatctataa taataccgac gtataattca tctgattaca ttactgaaac tctaacgaaa
    17941 ttagaaaaac aaacttaccc aaattttgaa attgttattg ttaatgatgg ttctaaagat
    18001 aacacatcaa acgttttgag agagtatggg ttaacccact ctcgattaat tattgtcaat
    18061 aaagaaaatg gcggtgtttc gtctgccagg aatacaggta tccgcaaggc gcaaggacag
    18121 tttatatgtt ttatggatga tgatgatgag atagatccta actatctgct gaagatgtat
    18181 accagacaac atgagacggg aggggatgcg atttattgtg ggctttatgg ccattatata
    18241 aaagatggtg ttacttactc acctataaat acagagttta atgaaggatc tttacttttc
    18301 gacttttttt ataaaaaggt tagattccat atagggtgct tgtttataag aaaacaactt
    18361 ctggaagaga ataatctttt ttttgatgaa gatttacgac taggagaaga tcttgatttt
    18421 atctatcgac tgctaattac atgcgatatg tatgcggttc catattatat gtataagcat
    18481 aactatagag aaaattcctt aatgaactca tgtagaacca tcactcatta tcgacatgag
    18541 tcatttgcgc acgaaagaat ctactcttct gtgatgcagt tatacaaagg taaccggaaa
    18601 gaagaaattc atacattatt gagtcaaaat agagcttatc ataaaactcg ttatttatgg
    18661 aatgttctac ttaatggtga ttttaaacta ttgaatcaat tagttgaaag caatgaaaaa
    18721 gaattaagag attgtaatct tcctggcaag agagataaga gacgagcaaa aatattagca
    18781 tcaaaaaatt atattctctg gaggatggta agactggtaa atagaaaaaa gaataaacgt
    18841 tagatcttgc attaaataac ccatcctcgt agcataggtt gaaattatat gtcgttttgt
    18901 tggaatgaaa ttaattctgg tataaagtct ttaattctta tattatgtat tttttcttta
    18961 atgactttgt ctttatggga tgatgttgca acaaagtttc ttcatgcagc tggaattata
    19021 tctgcattgt attttcttgc gacaccaaaa aaaacaataa ctaataatcc tactttgtta
    19081 attttcatct cattatgtct tttgggtatc gtaaatatca tctggtattc acattataaa
    19141 gtttcaggct ctgtttatac caatgcatat cgtggcccaa tggaaactgg aaaaattgcc
    19201 ttgtgtagcg cctttatttt cttagttctt tttgctaaaa atgagatgag aacaaaaata
    19261 aaatttggga aactaattct gttcgcatcc ctggcaacgc agttactttt ttttgcgcat
    19321 gccatgtggc aacatttcta tttaaacgtc gaccgtgttg cattatcagc ttcccacgct
    19381 acaacagcag gctacatcat cctttttcct tctttactgg catcaattct cattttaaaa
    19441 tccgacttta gacataaaac aacattatat acaattaact tcatgcttag cttatgtgct
    19501 gtcatagtaa ctgagacgcg tgcagccata ttagtgtttc cattctttgc gttaatatta
    19561 atcgtaatgg atagttatat taataagcga attaattata agttatattg ttttattgcg
    19621 attgcattat tagcaggtgt attttctttt aaagatacat tgcttatgag aatgaatgac
    19681 ttaaataacg atttagttaa ttattcgcat gataacacca gaacttcagt cggtgcccgt
    19741 ctggcaatgt atgaagttgg cttaaaaaca tattctccaa taggacaatc actggaaaaa
    19801 cgtgcagaaa aaatacatga gctagaagaa aaagagccta gattgagtgg cgctttacct
    19861 tttgtagatt ctcatttgca taacgatctc atagatacgt tatcaacgcg tggtattcct
    19921 ggagttgtat taacaatttt agcattttca gcaatactca tatatgcctt aagaactgct
    19981 aaagaacctt atattttaat cttgcttttt tcactactgg tagtaggcct aagtgatgta
    20041 atactctttt ctaaaccggt tccgactgct gtgtttgtca ccataatatt gctttgtgct
    20101 tattttaaag cacaatcaga ccaatattta ttagataagt aatgaatata tgaaataaaa
    20161 tcagcaagga ggctgatttt atttcaaaat tatctattaa ggggttgtaa tttaaatcat
    20221 agcagcgttt tcttcaatga atcgcttaac agcatttgct gtcaattgag acaactcatt
    20281 ccccggagcc ctacaaacca tttgattctt cccataccca ccaattaatc ccggatcggt
    20341 tggtccataa accgtgatat tgggtctatc cagtgccgcc gttaaatggc ttaaccccgt
    20401 atccaccgac actacaaatt tagccccggc cagcacgcgg gcaacgcctt ccagactcat
    20461 cttcggcaat acttcaacat aatcaaatcc ttccgccagt cgtttcgccc gttcttcctc
    20521 atgcggcgcg ccccacggaa gtttaatccg tattcctgaa tcagccagta aaccaatcaa
    20581 ttctcgccag tgttcttccg gccagtgttt atcatcacgg gtcgtcgcat gaagaaatac
    20641 ggcatattcg ccagcatctg taggcaggtt cgtcagaaaa tgctgtgcga tagcataatc
    20701 gccctgggtt tgcggtttgc tatagcccaa acttttggca aacagttcgc gggtgcgttc
    20761 tacggcgtgc tgctgttttg caatatgatg cttacaattg taaaacaggc tggctaaagg
    20821 ttcgcgagcg gtttgccagt ccaatccatg ctttacgcca tgcgccagac gtgtcaccag
    20881 tgccgcgctt tttaccagcc cctgagcgtc gataactgcg tcatagttct ctgcttgtag
    20941 cgcttcacga aacgctttgc gttcagcttt tatgggggcc gagaaccagg ctttacgcca
    21001 gcgacgtatt gccacaggaa taactcgctc aacggcagcg tgccaggaag gaatctgtgc
    21061 gaacccttct tccaccaccc agtcaaactt aatccctggg attgcctgct gggcatcagt
    21121 gagtgcgggc aacgtatgga gaacatcgcc catcgacgat gttttaacga tcaaaacccg
    21181 catccgtcag gcttcctctt gtaacaatag cgtgttgagt tcttccagta cgcgctgggg
    21241 agtaatgtcg atcaagctct ggtgataacc ctccgcagcg tcacctttac gcactttgtg
    21301 atagccggta atcagacgga tcacgcgcgc tttatgggat agcggcggtg tgaagtccgg
    21361 gctacttgga ccatacaggg caaccagcgg acgattgagc gccgccgcaa cgtgcatcag
    21421 gccagaatcg ttagtgacaa tggctttaca ggctgcaatc aggataaccg cttgatcaag
    21481 ctgtgtttcc cccgccaggt tccgacacca tgcctgctgc tcggtattca aagcggcaag
    21541 aatctcattg cccgcttcat gatctttcgc agagccaaac agaaccacct gataaccttc
    21601 atcaatcagc tgctttgcca gttccgcata gtggtagtgt ggccagcgtt ttgccggacc
    21661 aaactccgca cccgggcaaa agccaatcat cggacgttct gatgaaagcg aaaattgatt
    21721 acaggtatat gatttttcac cttcgctcac ctgcaactgc ggccataaca atggctgcgg
    21781 cagatcttgt gcggtacgca taatgccttt gtcataggcc agcgcgacat agcgttccac
    21841 cattagcggc caggcttctt tatcgagcac gcgtacatcg ttgagtaaac cgtagcgcat
    21901 ctcgccgcgc cagccggtgc gatgaggaat acccgcgaag aaaggcacta atgcagattt
    21961 gaaggagttg ggtaagacgt aggcgcggtc gtagcgcttt tcacgcaggc tatgacccag
    22021 tttgcggcgt tcgccgattt ccagcgctcc gtgaccgaga ggcataggga tagcttcgtt
    22081 aacttccggc atccgcgata ataatggacg gcaccatgcc ggtgccatca catcgattat
    22141 cgcctgggga tagcgcgcct ggagcgtgcg atagagactt tgcgacatca tcatgtcgcc
    22201 aacccaagac gggccgatca ccagtatttt catgcagagc tcttatgcgt cgcgattcag
    22261 ccaggccatg tattccgtta caccttcagc aacggttttg aacggtttgt cgtaacccgc
    22321 cgcgcgcaga tttgtcagat ctgcctgagt gaacgcctgg tagcggcctt tcagcttatc
    22381 cgggaacgga atgtattcga tctggccttt cttgtgataa gccagagtag catctgctac
    22441 ggcctggaag gattccgcac gaccagtacc gaggttgaag ataccggaaa cgccattttc
    22501 caggaaccac aggtttacat ctgccacgtc gcccacatag acgaagtcac gtttgaagtt
    22561 ctcgctacct tcaaacagct tcggtgattc accgttgtta agctgagtgt tgagatggaa
    22621 agcgacgctc gccatgctgc ctttatggcc ttcacgcggt ccataaacgt tgaaatagcg
    22681 gaagccaaca atctgcgagt tcgcttcagg caggatttgg cgaacatatt catcaaacag
    22741 gaattttgag taaccgtaga cgttcaacgg tttttcgtac tcgcgggatt caataaagtc
    22801 ggaggtgcgt ccgccgtagg tggctgcgga agaagcgtac aggaacggga tttcgcgctc
    22861 caggcagtag tgcagcagct ccttggagta ttgatagttg ttatccatca tatacttgcc
    22921 gtcccactcg gtggtggaag agcatgcgcc ttcgtggaaa atcgcttcga catcgccgaa
    22981 ctcttcgcca gccataatct ggatcaggaa gtcttcctta tccatatagt ccgcgatatc
    23041 cagatccacc aggttcacaa acttggtgcc gtctttcagg ttgtccacca ccagaatatc
    23101 ggtgatgcct ttatcattca gggccttaac gatgttgctg ccgataaagc ccgcgccgcc
    23161 ggtaacgatg atcataactg taaccttcga attatggagt cagagacaat ctcagacacg
    23221 aatacttcta tcatatcact atacggcgtt ggcttcagcc attcgccaaa agtgagattg
    23281 atggacgtta tttatcctga attttgcaga agtgttaacg cgtcatctcg tcgcgacgta
    23341 taagtttggg taatatgtgc tggaatttgc cctgtctgga gaatcgcaat gcgtggagaa
    23401 ttttatcagc agttaaccaa cgatctggaa accgcacggg cggaagggtt gtttaaagaa
    23461 gagcgcatta ttacgtctgc gcagcaagca gatatcactg tggctgatgg aagccacgtc
    23521 attaactttt gtgccaacaa ctatctcggg ctggcgaatc atccggatct gattgcggca
    23581 gcaaaggcgg gaatggattc tcacggtttc ggcatggctt cggtgcgttt tatttgcggc
    23641 actcaggaca gccataaaga gcttgaacaa aaactggcgg cattcctggg gatggaagat
    23701 gcgattctct actcttcctg ctttgatgct aacggtggac tgtttgaaac gctgctgggt
    23761 gcggaagacg ccattatctc cgacgcactg aaccacgcgt ctattattga tggtgtgcgt
    23821 ctgtgcaaag ctaaacgcta tcgctatgcc aacaacgata tgcaggagct ggaagcacgt
    23881 ctgaaagaag cgcgtgaagc cggtgcgcgt catgtgctga ttgccaccga tggtgtgttc
    23941 tcaatggacg gcgtgattgc caacctgaaa ggcgtttgcg atctggcaga taaatacgat
    24001 gccttggtga tggtggatga ctcccacgcg gtcggttttg tcggtgaaaa tggtcgtggt
    24061 tcccatgaat actgcgatgt gatgggccgg gtcgatatta tcaccggtac gcttggtaaa
    24121 gcgctgggcg gggcttctgg tggttatacc gcggcgcgca aagaggtggt tgagtggctg
    24181 cgccagcgtt ctcgtccgta cctgttctcc aactcgctgg caccggccat tgttgccgcg
    24241 tccatcaaag tactggagat ggtcgaagcg ggcagcgaac tgcgtgaccg tctgtgggcg
    24301 aacgcgcgtc agttccgtga gcaaatgtcg gcggcgggct ttaccctggc gggagccgat
    24361 cacgccatta ttccggtcat gcttggtgat gcggtagtgg cgcagaaatt tgcccgagag
    24421 ctgcaaaaag agggcattta cgtcaccggt ttcttctatc cggtcgttcc gaaaggtcag
    24481 gcgcgtattc gtacccagat gtctgcggcg catacccctg agcaaattac gcgtgcagta
    24541 gaagcattca cgcgtattgg taaacaactg ggcgttatcg cctgaggatg tgagatgaaa
    24601 gcgttatcca aactgaaagc ggaagagggc atctggatga ccgacgtccc tgtaccggaa
    24661 ctcgggcata acgatctgct gattaaaatc cgtaaaacag ccatctgcgg gactgacgtt
    24721 cacatctata actgggatga gtggtcgcaa aaaaccatcc cggtgccgat ggtcgtaggc
    24781 catgaatatg tcggtgaagt ggtaggtatt ggtcaggaag tgaaaggctt caaaatcggc
    24841 gatcgcgttt ctggcgaagg ccatatcacc tgcggtcact gtcgcaactg tcgtggcggt
    24901 cgtacccatc tgtgccgcaa cacgatcggc gtcggcgtta accgtccggg ctgcttcgcc
    24961 gaatatctgg tgatcccggc gttcaacgcc ttcaaaatcc cagacaatat ctccgacgac
    25021 ctggcttcca tttttgatcc cttcggtaac gccgtgcata ccgcgctgtc gttcgatctg
    25081 gttggcgagg atgtgctggt ctctggtgca ggtccgatag gtattatggc tgcggcggtg
    25141 gcgaaacacg ttggtgcacg caatgtggtg atcactgatg ttaacgaata ccgccttgag
    25201 ctggcgcgca aaatgggtat cacccgtgcg gttaacgtcg cgaaagaaaa ccttaatgat
    25261 gtgatggctg aactgggcat gaccgaaggc tttgatgtcg gtctggaaat gtccggtgcg
    25321 ccgccagcgt ttcgtaccat gcttgacacc atgaaccacg gcggccgtat tgcgatgctg
    25381 ggtattccgc cgtctgatat gtctatcgac tggaccaaag tgatctttaa aggcttgttc
    25441 attaaaggta tttacggtcg tgagatgttt gaaacctggt acaagatggc ggcgctgatt
    25501 cagtctggcc tcgatctctc gccgatcatt acccatcgtt tctctatcga tgatttccag
    25561 aagggctttg acgctatgcg ttcgggccag tccgggaaag taattctgag ctgggattaa
    25621 cacgaacaag ggctggtatt ccagcccttt tatctgggga taatctgttg aatatgtaaa
    25681 atcctgtcag tgtaataaag agttcgtaat tgcgctgatc tcttatatag ctgttctcat
    25741 tatctctcta ccctgaagtg actctctcac ctgtaaaaat aatatctcac aggcttaata
    25801 gtttcttaat acaaagcctg taaaacgtca ggataacttc agaggtcgtc ggtaatttat
    25861 gatgaacagc accaataaac ttagtgttat tattccgtta tataatgcgg gcgatgattt
    25921 ccgcacttgt atggaatctt taattacgca aacctagact gctctggaaa tcattattat
    25981 taacgatggt tcaacggata attctgttga aatagcaaag cattacgcag aaaactatcc
    26041 gcacgttcgt ttgttgcatc aggcgaatgc tggcgcatcg gtagcgcgta atcgtgggat
    26101 cgaggtggcg acgggcaaat atgtcgcttt tgtcgatgct gacgatgagg tctatcccac
    26161 catgtacgaa acgctgatga ccatggcgtt agaggacgac ctcgacgtgg cgcagtgcaa
    26221 cgctgactgg tcttttcgtg aaacgggaga aacctggcaa tccatcccca gcgatcgcct
    26281 tcgctcaacc ggcgtattaa ccggcccgga ctggctgcgg atggggcttt cttcgcgccg
    26341 ttggactcac gttgtctgga tgggggttta tcgccgtgat gttattgtta aaaataacat
    26401 taaatttatt gccggattac atcatcagga tattgtctgg acgacagaat tcatgtttaa
    26461 cgcgctgcgt gcgcgatata ccgagcaatc attatataaa tattatctgc ataatacgtc
    26521 agtgagtcgg ttgcatagac aagggaataa aaaccttaat tatcaacgtc actatattaa
    26581 gattacccgc ctgctggaga aattaaatcg aaattatgcc gacaaaatta cgatttatcc
    26641 ggaatttcat cagcaaataa cttacgaagc attgcgtgtt tgccatgcgg tgcgcaaaga
    26701 gccggatatt cttacccgcc aacggatgat tgccgagata tttacttccg gtatgtataa
    26761 gcgcctgatt accaatgtgc gcagcgtgaa ggtcggttac caggcgttac tgtggtcttt
    26821 ccgcttatgg caatggcgcg acaaaacgcg gtcgcaccat cgcattacgc gtagcgcctt
    26881 taatttgcgc tagcgttaaa cttcgccgct tcgggctgtt tgccccagcc ttgccactga
    26941 tgctggaagt aaacgatcag cgtgctctgg ctgatgcttt cgcttaatac ttcaaagaag
    27001 cgattagcat aaacaggttc tatcggtttc ttcggtttgc acagcttcac gccacggaac
    27061 ggattacgcg gtgcatcagg cacagcgttt ttcggcggtg tagaagtatc aacctgcggt
    27121 tcattcagca agctgctggc tttcaccagc gtaatgtctg gcggcaggtt ataaaccatc
    27181 tgttgtagca cgcgtaccgt taaagggtga ggatgcccaa tggcaatggt tgaaccgttg
    27241 cgacgcgcca gatcaattgc gcgattaaat tgcacacgga tgtccgcttc gttctgcgaa
    27301 tcgtcgagga acaccttccg tttaataacc ttcacgccag taccttgcgc ggcgcgcatc
    27361 gcctgggtat taccgatggt tacgctgtcg aggaagtaaa gattgtaacg ctccagtgcc
    27421 tgcatcactt tctgcatacc aaacaggtta gaggtcatct tgctacccat gtggttgttg
    27481 atccccacgg catagggcac gttattgacc gcactacgaa taatgcgctc aatttcgtcg
    27541 ctgctcatct ccgggcgcag cgtatttttc tccagcggct gtttactcag tggtgccatc
    27601 gggagatgaa tcaacacttc gtgcccgctg ttatgcgctt tggtcgccat ctctctggcg
    27661 tgcggtgaat cgggtaatac agcgacggag atagcggagg gcatcgccag cacctggttt
    27721 tcgttgtgcg ggcgataccc aaaatcatca atgacgatgg caagtttgcc agcaagtacg
    27781 ggggaggaga gcgccaacag agcggcaaat gcaagaacgt tacgacgaaa tggaaacaaa
    27841 acttatcttc ccaaccacgg ctgtggattg accgcctgac cctggcggcg aatttcgaaa
    27901 tagagtgaag gccgaccctg accgccactg ctgcccacca gtgcaattgg ctggcccgcg
    27961 cgaacctgcg aaccaacgct caccagtgcg ctctgattat agccgtaaag actcatgtcg
    28021 cctttaccgt gctcaaccac caccaccaga ccgtaacctt gcagccagtc agccagaatc
    28081 actcgaccgt cggcaattgc tttaacttca gtaccttcag aggcaccgat aaccatccct
    28141 ttccagcgta attcaccctg tagctgctcg ccatagcgat gcagcgtcgg cccgcgaacc
    28201 ggccagaatg cctgaccacg cggcgcaccc agaccaccag ttcgggacat cagcgatttt
    28261 tcgctttcgg tcggtttgta ggtggtgcct ttgcgcgtcg cttctttctg gcggtcgcga
    28321 accgcctggg cctcacgcgc ttctcgttca gcacgcgctt tcgccgcggc ttccgcacgg
    28381 gcaatgctgt tacgcagacg ggattcgttg gcacgcagtt cacttagctg ctgctgacct
    28441 tgctggatgg aagattccag ccccgccagc gtctttttac gctcgttcag agcctgagtc
    28501 agtttcgcct gttgggcgcg ctgttcatat aacagcgttt gttgctcgct ttgtttctct
    28561 tccagttcag cacgctgcat ggcgacttct tcacgcgttt gcttcaactg ggcaatggtt
    28621 tcttgtcgcg cctggttgag atagccgaaa taagcctgta aacgctgtcc acactggctt
    28681 tcttcaccgc tgagaattag ctgaataccg gtatgctcgc cctgacggaa tgcggcatcc
    28741 agttgtgcgg cgaggctgcg ctcctgggcg gctttttgct gctccagttt ggcaatcgac
    28801 gcgttcatct catcaatctg tttattgagt tgattgagcg tgttttgcgt ttcgcgcagc
    28861 ttacgggtgg cttcagagat cgcttcttcc tgctttttca attgtgcgag caggcttgcg
    28921 cgttgttgtt gcttttggcg taccgcgcgc tcttttgcgg cgatatcggc ctgaatagat
    28981 ttgagttggt cacgctcatc cgcgtgggcg gaaaaggcgc acaacaatac gccagcgcta
    29041 agaacgctgg cgtagatgat gggcctgatt gcaaacctgc gcggtttcac ggcccgtgtc
    29101 atggtattaa tcgcctttcc cctcatgggg agggattatt ccacgatgaa cagcggctta
    29161 ccagtcatct cttgcgggat ttccataccc atcagcgaca acatggtcgg cgcgatgtca
    29221 gaaagtttgc cgcctgcaac cgctttcacg ttcttatcac caacgtaaat cagcggaact
    29281 ggcaggttgg tgtgtgccgt gtgtgcctga cccgttgccg gatcgcgcat ctgttcagcg
    29341 ttaccgtggt cagcggtgat cagcagttgt ccacccacgg actcaaccgc tttcgcgact
    29401 tcttccacgc agtgatccag tgcttcaacc gctttaaccg ccgcttccat caccccggtg
    29461 tgacctacca tgtcgccgtt cggatagtta cagataatgg tgtcgtattt gccgctcttg
    29521 atggccgcaa ccagtttttc ggtcagctct gcggagctca tttccggttg cagatcgtag
    29581 gtagccactt tcggtgagtt gatcagaatg cgatcttcgc ctttgaatga ctcttctacg
    29641 ccaccgttga agaagaaagt aacgtgggca tatttttcgg tttcggaaat acgcaactga
    29701 gttttgtcgt ttttcgccat ccactcgccg aaggtgttaa ccagggaagc gggtgggtaa
    29761 gcaaccgcag ttttgatgtc agcggcgtat tcggtcagca tcacgaaatc gacgttaacc
    29821 actttcttac gcgcgaagcc gtcgaaatct gcgtttacga atgcacgagt gatttcgcgc
    29881 gcgcggtcag cacggaagtt catgaaaatc agcgcatcgc cgtcttccat tgccgcatct
    29941 ggctgacctt ctgcacgaat aacggtcgct ttcacgaact cgtcgttttc gtcgcgagca
    30001 taagctgcct gcaaaccagc aacggcagta tcggcctgga actcaccctg cgccagagtc
    30061 agcaggtcat aggctttttc aacgcgatcc caacggttat cgcggtccat cgcgtagtaa
    30121 cgaccaatga tggacgctac gcggccttta cccagcgcag caaatttctc ttcaaatttt
    30181 ttcagcgagg attcagcact gcgcggcggg gtgtcgcgac cgtcaaggaa tgcgtgcagg
    30241 tagatttttt ctgcgccgcg ttcagctgcc agttctacca tcgccatgat gtgatcttcg
    30301 tggctgtgta cgccgcctgc ggagagcaga cccataatgt gtactgcttt acctgcgttt
    30361 ttcgctttat ctaccgcgcc agtcagcact ggattagcaa agaaagcgcg atctttgatt
    30421 tcaacgtcca gacgagtcag gtcctgatac acgatgcggc cggcacccag gttaacgtgg
    30481 cctacttcgg agttacccat ctgacggtca ggcagaccga cttccagacc ggaagcgtcg
    30541 atcagggtat gcggacgatt ggcccacagt gcatccatta ccggggtttt agcactaaaa
    30601 atggcgttat cctgctgttc ttcacgatag ccatagccat ccagaatcac cagtaccata
    30661 ggttttttag aaaccgacat tgcgacaacc tcatactcaa gagtcaaaat ttgcgtaatt
    30721 ttactacagc tgaatcgata aaatagcctc tgaagatcaa aatatacgac cgctcgcggc
    30781 aagccgatgc cagatttgcg cttttttttc gtcgtgcccc gcaaaattgc attccagtta
    30841 acgcgctggc tgtatttgcc gcaccgcgca ggtatactcc tttcctggtt tttttaatca
    30901 ctacgtcggg agttgttacc ccccatgcaa gaaattatgc aatttgttgg ccgtcatccc
    30961 atactgagta tcgcttggat cgcgttactg gtggcggttc ttgtgactac gtttaagagc
    31021 ctgacctcga aagtgaaggt gattactcgt ggtgaagcta cgcgtctgat caacaaagaa
    31081 gacgctgtgg ttgtggattt acgtcagcgt gatgacttcc gtaaaggcca tatcgcaggt
    31141 tctatcaatc tgttgccgag cgaaatcaaa gccaacaatg ttggtgagct tgagaagcac
    31201 aaagacaaac cggttatcgt ggtagacggt tctggcatgc agtgccagga gcctgcaaac
    31261 gcgctgacga aagctggttt tgcgcaagta ttcgtgctga aagagggcgt tgctggctgg
    31321 gctggcgaaa acttgcctct ggtgcgcggc aaataattta cctgtcgtct ttcatgatgc
    31381 aggccgttag ctgcaattag gcccccagcg acttattgtt ataagcttct ggggacactc
    31441 cattttgcag ccgccctgca acatgaaatt tcagggtaaa aaaacaggag ctaaattatg
    31501 gccaatgttg aaatctatac caaagaaacc tgcccgtatt gccatcgtgc aaaagcactg
    31561 ctgagcagca agggcgtgag ttttcaggag ttgccgatcg atggcaatgc cgccaagcgt
    31621 gaagagatga tcaaacgcag cggtcgcacc acggttcccc agatttttat tgacgcacag
    31681 cacattggcg gctgtgatga cttgtatgca ttggatgcac gtggtggact ggatcccctg
    31741 ctgaaataac gtgtgaacgt tggcattaca ttgcgcagta tttaaggaca acacttaagg
    31801 gttttctaca catgtcagaa caaaacaaca ctgaaatgac tttccagatc caacgtattt
    31861 ataccaagga tatctctttc gaagcgccga acgcgccgca cgttttccag aaagattggc
    31921 aaccagaagt taaacttgat ctggatacgg catcttccca actggcagat gacgtatacg
    31981 aagtggtact gcgtgttacc gtaacggcct ccctgggcga agaaaccgcg ttcctgtgtg
    32041 aagttcagca gggcggtatt ttctccatcg cgggtatcga aggcacccag atggcacatt
    32101 gcctgggcgc atactgcccg aacattctgt tcccgtatgc tcgtgagtgc atcaccagca
    32161 tggtatcccg cggtacattc ccgcaactga accttgcgcc ggttaacttc gatgcgctgt
    32221 tcatgaacta tttgcagcag caggctggcg aaggtactga agaacatcag gatgcctgat
    32281 gaaccaacgt aatgcttcaa tgactgtgat cggtgccggc tcgtacggca ccgctcttgc
    32341 cattaccctg gcaagaaatg gccacgaggt tgtcctctgg ggccatgacc ctgaacatat
    32401 cgcaacgctt gaacgcgacc gctgtaacgc cgcgtttctc cccgatgtgc cttttcccga
    32461 tacgctccat cttgaaagcg atctcgccac tgcgctggca gccagccgta atattctcgt
    32521 cgtcgtaccc agccatgtct ttggtgaagt gctgcgccag attaaaccgc tgatgcgtcc
    32581 tgatgcgcgt ctggtgtggg cgaccaaagg gctggaagcg gaaaccggac gtctgttaca
    32641 ggacgtggcg cgtgaggcct taggcgatca aattccgctg gcggttatct ctggcccaac
    32701 gtttgcgaaa gaactggcgg caggtttacc gacggctatt tcgttggcct ctaccgacca
    32761 gacctttgcc gatgatctcc aacaattgct gcactgtggc aaaagtttcc gcgtttacag
    32821 caacccggat ttcatcggtg tgcagcttgg cggtgcggtg aaaaacgtca ttgccattgg
    32881 cgcggggatg tccgacggta tcggttttgg tgcgaatgcg cgtacggcac tgatcacccg
    32941 tgggctggct gaaatgtcgc gtcttggcgc ggcgctgggt gccgagcctg ccacctttat
    33001 gggcatggcg gggctgggcg atcttgtgct tacctgtacc gacaaccagt cgcgtaaccg
    33061 ccgttttggc atgatgctcg gtcagggcat ggatgtacaa agcgcgcagg agaagattgg
    33121 tcaggtggtg gaaggctacc gcaatacgaa agaagtccgc gaactggcgc atcgcttcgg
    33181 cgtagaaatg ccaataaccg aggaaattta tcaagtatta tattgcggaa aaaacgcgcg
    33241 cgaggcagca ttgacgttat taggtcgtgc acgcaaggac gagcgcagca gccactaacc
    33301 ccagggaacc tttgttaccg ctatgacccg gcccgcgcag aacgggccgg tcattatctc
    33361 atcgtgtgga gtaagtaatg tcgtgtgaag aactggaaat tgtctggaac aatattaaag
    33421 ccgaagccag aacgctggcg gactgtgagc caatgctggc cagtttttac cacgcgacgc
    33481 tactcaagca cgaaaacctt ggtagtgcac tgagctacat gctggcgaac aagctgtcat
    33541 cgccaattat gcctgctatt gctatccgtg aagtggtgga agaagcctac gccgctgacc
    33601 cggaaatgat cgcctctgcg gcctgtgata ttcaggcggt gcgtacccgc gacccggcag
    33661 tcgataaata ctcaaccccg ttgttatacc tgaagggttt tcatgccttg caggcctatc
    33721 gcatcggtca ctggttgtgg aatcaggggc gtcgcgcatt ggcaatcttt ctgcaaaacc
    33781 aggtttctgt gacgttccag gtcgatattc acccggcagc aaaaattggc cgtggcatta
    33841 tgctcgacca cgccacgggc atcgttgtgg gtgaaacggc ggtaattgaa aacgacgtat
    33901 cgattctgca atctgtgacg cttggcggta cgggtaaatc tggtggtgac cgtcacccga
    33961 aaattcgtga aggtgtgatg attggcgcgg gcgcgaaaat cctcggcaat attgaagttg
    34021 gtcgcggcgc gaagattggc gcaggttccg tggtgctgca accggtgccg ccgcatacaa
    34081 ccgccgctgg cgttccggct cgtattgtcg gtaaaccaga cagcgataag ccatcaatgg
    34141 atatggacca gcatttcaac ggtattaacc atacatttga gtatggggat gggatctaat
    34201 gtcctgtgat cgtgccggat gcgatgtaat catctatccg gcatacagta actaatctct
    34261 caacaacgct cccggatatc ccagctgccg ccaggcttca tacaccacta ccgacaccgc
    34321 attggacaga ttcatgctgc ggctgtccgg caccatcgga atgcgaattt tttgttcagc
    34381 gggcagggca tcaagaatgc tcgctggcag gccgcgtgtt tccgggccga acatcagata
    34441 atcgccatcc tgatagctta cggcgctgtg agcaggcgta cctttcgtgg tgagggcaaa
    34501 caggcgctgg ggattttctg cttcgaggaa cgcgcgatag tcatgatgac gcgtaacggc
    34561 ggtaaactcg tgatagtcca gccccgcgcg gcgcaggcgc ttatcgtccc aggcaaatcc
    34621 catcggttcg atgatatgca gacgaaagcc ggtattagcg caaagacgga tgatgttgcc
    34681 agtatttggc ggaatttctg gttcgtaaag tacgatgttt agcatactgc ccccttagtg
    34741 cgggggcagg atagcagaaa attggtgaac cgtgcggcct tttcccctca ccccggccct
    34801 ccccctgtct cttgatcagg agatctgatc aagagacagc tccctgaggg agagggt
//
LOCUS       DAJTNN010000022        34612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTNN010000022 DAJTNN010000000
VERSION     DAJTNN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.022"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..334)
                     /locus_tag="OO150_000956"
     CDS             complement(<1..334)
                     /locus_tag="OO150_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5515184.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGE"
     gene            complement(415..1842)
                     /locus_tag="OO150_000957"
     CDS             complement(415..1842)
                     /locus_tag="OO150_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5515185.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETEPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1839..2546)
                     /locus_tag="OO150_000958"
     CDS             complement(1839..2546)
                     /locus_tag="OO150_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5515186.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2709..3686
                     /locus_tag="OO150_000959"
     CDS             2709..3686
                     /locus_tag="OO150_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5515187.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3756..4238)
                     /locus_tag="OO150_000960"
     CDS             complement(3756..4238)
                     /locus_tag="OO150_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5515188.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4473..7055
                     /gene="leuS"
                     /locus_tag="OO150_000961"
     CDS             4473..7055
                     /gene="leuS"
                     /locus_tag="OO150_000961"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5515189.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7070..7651
                     /gene="lptE"
                     /locus_tag="OO150_000962"
     CDS             7070..7651
                     /gene="lptE"
                     /locus_tag="OO150_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5515190.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7651..8682
                     /gene="holA"
                     /locus_tag="OO150_000963"
     CDS             7651..8682
                     /gene="holA"
                     /locus_tag="OO150_000963"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5515191.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8684..9325
                     /gene="nadD"
                     /locus_tag="OO150_000964"
     CDS             8684..9325
                     /gene="nadD"
                     /locus_tag="OO150_000964"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5515192.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9349..9957
                     /locus_tag="OO150_000965"
     CDS             9349..9957
                     /locus_tag="OO150_000965"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5515193.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPPLTARGIEQAQNLHTLLHGV
                     SFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAENY
                     SAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARLI
                     GMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVDNA"
     gene            10217..10534
                     /gene="rsfS"
                     /locus_tag="OO150_000966"
                     /gene_synonym="rsfA"
     CDS             10217..10534
                     /gene="rsfS"
                     /locus_tag="OO150_000966"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5515194.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10538..11005
                     /gene="rlmH"
                     /locus_tag="OO150_000967"
     CDS             10538..11005
                     /gene="rlmH"
                     /locus_tag="OO150_000967"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5515195.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11036..12937
                     /gene="mrdA"
                     /locus_tag="OO150_000968"
     CDS             11036..12937
                     /gene="mrdA"
                     /locus_tag="OO150_000968"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5515196.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTTHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12940..14052
                     /gene="mrdB"
                     /locus_tag="OO150_000969"
                     /gene_synonym="rodA"
     CDS             12940..14052
                     /gene="mrdB"
                     /locus_tag="OO150_000969"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5515197.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14063..15151
                     /gene="rlpA"
                     /locus_tag="OO150_000970"
     CDS             14063..15151
                     /gene="rlpA"
                     /locus_tag="OO150_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5515198.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15291..16502
                     /gene="dacA"
                     /locus_tag="OO150_000971"
     CDS             15291..16502
                     /gene="dacA"
                     /locus_tag="OO150_000971"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5515199.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16613..16876
                     /locus_tag="OO150_000972"
     CDS             16613..16876
                     /locus_tag="OO150_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5515200.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVEILYEELGKIDIVRMVL"
     gene            16977..17618
                     /gene="lipB"
                     /locus_tag="OO150_000973"
     CDS             16977..17618
                     /gene="lipB"
                     /locus_tag="OO150_000973"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5515201.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17877..18830
                     /locus_tag="OO150_000974"
     CDS             17877..18830
                     /locus_tag="OO150_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5515202.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19039..20004
                     /gene="lipA"
                     /locus_tag="OO150_000975"
     CDS             19039..20004
                     /gene="lipA"
                     /locus_tag="OO150_000975"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5515203.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20105..20308)
                     /gene="tatE"
                     /locus_tag="OO150_000976"
     CDS             complement(20105..20308)
                     /gene="tatE"
                     /locus_tag="OO150_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5515204.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20437..21225)
                     /locus_tag="OO150_000977"
     CDS             complement(20437..21225)
                     /locus_tag="OO150_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5515205.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21318..21701
                     /gene="crcB"
                     /locus_tag="OO150_000978"
     CDS             21318..21701
                     /gene="crcB"
                     /locus_tag="OO150_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5515206.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21756..21965)
                     /gene="cspE"
                     /locus_tag="OO150_000979"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21756..21965)
                     /gene="cspE"
                     /locus_tag="OO150_000979"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5515207.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22140..22700)
                     /gene="pagP"
                     /locus_tag="OO150_000980"
     CDS             complement(22140..22700)
                     /gene="pagP"
                     /locus_tag="OO150_000980"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5515208.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23289..24674
                     /gene="dcuC"
                     /locus_tag="OO150_000981"
     CDS             23289..24674
                     /gene="dcuC"
                     /locus_tag="OO150_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5515209.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24715..25395)
                     /gene="dpiA"
                     /locus_tag="OO150_000982"
                     /gene_synonym="citB"
     CDS             complement(24715..25395)
                     /gene="dpiA"
                     /locus_tag="OO150_000982"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR5515210.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25364..27021)
                     /gene="dpiB"
                     /locus_tag="OO150_000983"
                     /pseudo
     CDS             complement(25364..27021)
                     /gene="dpiB"
                     /locus_tag="OO150_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            27370..28428
                     /gene="citC"
                     /locus_tag="OO150_000984"
     CDS             27370..28428
                     /gene="citC"
                     /locus_tag="OO150_000984"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5515211.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            28443..28739
                     /gene="citD"
                     /locus_tag="OO150_000985"
     CDS             28443..28739
                     /gene="citD"
                     /locus_tag="OO150_000985"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5515212.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGTLDCILRARLEALLARASGIPTLPWEDCQ"
     gene            28736..29644
                     /gene="citE"
                     /locus_tag="OO150_000986"
     CDS             28736..29644
                     /gene="citE"
                     /locus_tag="OO150_000986"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5515213.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29655..31187
                     /gene="citF"
                     /locus_tag="OO150_000987"
     CDS             29655..31187
                     /gene="citF"
                     /locus_tag="OO150_000987"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5515214.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIHRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQEQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            31191..31741
                     /gene="citX"
                     /locus_tag="OO150_000988"
                     /pseudo
     CDS             31191..31741
                     /gene="citX"
                     /locus_tag="OO150_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            31716..32593
                     /gene="citG"
                     /locus_tag="OO150_000989"
                     /pseudo
     CDS             31716..32593
                     /gene="citG"
                     /locus_tag="OO150_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
     gene            32644..34107
                     /gene="citT"
                     /locus_tag="OO150_000990"
     CDS             32644..34107
                     /gene="citT"
                     /locus_tag="OO150_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR5515215.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            34218..>34612
                     /locus_tag="OO150_000991"
     CDS             34218..>34612
                     /locus_tag="OO150_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5515216.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETA"
BASE COUNT         8598 a         8830 c         8562 g         8622 t
ORIGIN      
        1 cttccccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
       61 agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata
      121 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
      181 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttata ccggtataca
      241 tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa
      301 gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt aagtgcatta
      361 actgactact tttttatatt acttgccggg ctttccgcac attaacgtcg actgtcaccc
      421 gaacagatgg agtattttcc ctatcaaacc ggcaaagata aaaatgtaga aaataatttt
      481 gatgacgccc agacagcccg gcccaccatc gtcaacgggc attggctgcg gttgcggctc
      541 cggttcaaca gtttgcttct cggtaaaatt cgccgcctga attaataatc gacgaataat
      601 taatgcttcc agtggacaac gatcttctgc atcaagacgg cttacgatcg gcattaatga
      661 ttcttctgtc ggttggctgg cctgcactaa ttgtttggca actaaatata atggccataa
      721 gagatgttct gtatcacgcg tgagtgagat ccgcggctta cgtcctttgt gcaaaaccat
      781 ctcgaccagt gaacgcccca gcgcagagag tttttcatcg ctgagtattt cgtataaatg
      841 aaaacgcgta cgggcgctgt cgccggggac ataaagtagc tgtttaccct gatcgttaac
      901 cagttcacga gcttcaatca tcatcaccac taacgggtaa taatcgaccc gatgctgacg
      961 gcaccattgc aatattaaag catccgcctg ggtggaggga tactcgcgcc agtagtcaca
     1021 aagttctgcc agcaagcttt cgccttctcc acaatagacg cgttgcgcgt attcataata
     1081 acgcgcatct tcattatcag gttcagcttc ccgccacgac cgggcgtaat ccagaagttc
     1141 ggggatatcc aggcgacctg cgctatacca gcggagtaaa tttctgtgca gttttgcatc
     1201 atcggggatg atcactgcgc gatgttgcgc taaatacaat gtcagccatt ctggatgcga
     1261 tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg tcattttgca cggcaacggg
     1321 cagatgcaga atggtctgga agttaaaaac attccccttc gcaatggcat ataaaaagga
     1381 ttccagttct tcttcgtcta tttccccgac gttttcctcc tgcaaccacc gcaatctttc
     1441 tgctaataac accacgcact cgaaggataa atgggcagtg ttcatggcga ttgtgcacag
     1501 cgaccagcgt aattcatcaa tctcttccat cgagcaataa tttaattgct gaatgaatcg
     1561 ctgccaggcg gagggcagaa aacgttcgct atcagaggca agtaacgcgc gaaaggcgag
     1621 cagaatttca tgttctgcta cctcatattc ttctggttgc caaacagatt tagcaggcga
     1681 ctgcgcaatc cgtagcgcct cctcatacgc ctgacgaagt tgtttaaaac cctgcggttc
     1741 ggtttctgga tgaaaggacg gtaacagcgc cagataagcg cggcggataa tatcgacgtc
     1801 agtcgtttcc tctatatcga ggatcttcca gcaatttttc ataagtcgct catatcctta
     1861 agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc aggacaaggt agggtaatac
     1921 gcatcatcac tggcaaataa ttgataaaac atatcattgc cggaatcgtc gtattcttta
     1981 cgcgctaaga attcgcgtaa taaaggcaga tgatcatctt cgggagactg ccaatattgc
     2041 cgaccaaagg tataaccggc aaagtattgc cgccagttac tataataacg tagagcccgt
     2101 agatgaatac gcgactggat ccatagactt tcttcttcac tcaaccagtt attaagtacg
     2161 cccattcgac ttaaaaaacc catccggaca taatcccatg ctttaattcc tccctcacca
     2221 cagcattcgg ctgtgctggc gacaaattgc gcataagcct gaccttgctc atttaattca
     2281 gcaagatagt cacgccattc acatggagaa taacgaaacc agcggtgata aaaccctgcc
     2341 aaccgagcgg catgaccgtt ctcgatcatt cggtgaattg tgcgcagcag gtcatcacgg
     2401 tcatcaattc cccatgagga agaaaggtca aaaaagcatg tacctaaata cattttgggg
     2461 gatgaatgcg aaggtaaaca gcattcattg cgtatttcca tcggagtgga aagtgcaaac
     2521 aatatttttt gcgaccccat gtccatataa tttatcctta aacattcatt taatgaatgt
     2581 aaactacatt tcgagactca gttatttata cttttctgac gcgacataat acgcataaaa
     2641 taaggcttac gcaaaaatgg aatacataaa atataatatg ttttatctac aaccataagg
     2701 aaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
     2761 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
     2821 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgccg
     2881 agcaaggtca ttgtgaagca caatattcct taggacaaaa gtatactgag gataaaagcc
     2941 gtcataaaga taatgagcat gccatctttt ggctgaaaaa aactgcccta caaggccata
     3001 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagacccc aattataaag
     3061 aagcggttgt ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
     3121 ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga ctatgcgctg gcatttttct
     3181 ggtacaaaca agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt
     3241 atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
     3301 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
     3361 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
     3421 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
     3481 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
     3541 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
     3601 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
     3661 ttcgtgagac tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc
     3721 ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
     3781 aactggtcat gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
     3841 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
     3901 accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg
     3961 atatctgcca gctcctgcca taagctttct ttaatcaccc gcataaagac gctgtcagat
     4021 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tcttacagct
     4081 cgcgtcagct catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
     4141 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac
     4201 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
     4261 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
     4321 taaattaatg tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
     4381 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
     4441 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
     4501 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
     4561 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
     4621 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgctaccag cgtatgctgg
     4681 gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
     4741 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
     4801 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
     4861 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
     4921 ataagaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
     4981 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
     5041 agtggtttat caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg
     5101 atcactggcc agacaccgtt aaaagcatgc agcgtaactg gatcggtcgt tccgaaggcg
     5161 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
     5221 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
     5281 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
     5341 ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg
     5401 ttcacccatt aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt
     5461 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
     5521 ctaaatacgg cctgaacatc aaaccagtta tcctggctgc tgacggctct gagccagatc
     5581 tttctcagca agccctgact gaaaaaggcg tgctgttcaa ctctggcgaa ttcaacggtc
     5641 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
     5701 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg
     5761 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc
     5821 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
     5881 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
     5941 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
     6001 aaggtatgct ggattccgaa gcagctaact actggctgcc ggtggatatc tacattggtg
     6061 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
     6121 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc
     6181 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
     6241 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
     6301 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
     6361 cacaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
     6421 ctccggctga tatgactcta gaatggcagg aatccggtgt ggaaggggct aaccgctttc
     6481 tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa aggtgatgtt gcggcactga
     6541 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaacgatcg
     6601 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
     6661 tggagctgat gaacaaactg gcgaaagcac caaccgatgg cgagcaggat cgcgctctga
     6721 tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc gttcaccccg cacatctgct
     6781 tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg
     6841 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
     6901 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
     6961 gccaggaaca tctggtagca aaatatcttg atggcgttac cgtgcgtaaa gtgatttacg
     7021 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
     7081 ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
     7141 tgataccacg caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa
     7201 cgggccatta agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
     7261 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
     7321 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
     7381 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
     7441 ttcgttcttt gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
     7501 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
     7561 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
     7621 tgcacgcgtc tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc
     7681 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
     7741 ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt cgaagaacac
     7801 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
     7861 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
     7921 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgacga cctgctgttg
     7981 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt
     8041 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
     8101 gttgctgcgc gcgcaaaaca gctcaactta gaactggatg acgcggcaaa tcaggtgctc
     8161 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
     8221 ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
     8281 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcacattg
     8341 catattcttc agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta
     8401 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
     8461 gcgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
     8521 aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
     8581 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
     8641 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
     8701 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct
     8761 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
     8821 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga
     8881 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
     8941 gcaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
     9001 tggtcaggat tcactgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
     9061 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
     9121 ccagcaatgg ctggaagatc atttgacaca taaccaggaa gatcttcacc ttcagcctgc
     9181 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
     9241 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtac tgacttacat
     9301 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
     9361 ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc tttacagcgg tcatgcgccc
     9421 cccctgaccg cgcgcggtat tgagcaagcg caaaatctgc atacgctgct acatggtgtt
     9481 tcctttgatc tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt
     9541 ctcagtgacc gccagctccc cgtgcaaatc atccctgaac tcaacgaaat gttttttggc
     9601 gactgggaga tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg
     9661 tggtgcaatg actggcagca tgcaatcccc acgaacggtg aaggatttca ggcattttcg
     9721 caacgtgtgg aacgctttat cgcaaggctt agtgaatttc agcactatca gaatatttta
     9781 gtcgtcagcc atcagggtgt actgagtctg ttaatcgccc gtttaattgg catgcctgcc
     9841 gaggctatgt ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa
     9901 aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgataa tgcatgactt
     9961 ttctgttttt ttacgggtaa gccgcaacga ccattgacag acccgggcag gctgatattc
    10021 tccgcagcca gacttttttc gccagacacg actttgtaga aattgtttta caaaaatggc
    10081 gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca
    10141 attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt
    10201 cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga
    10261 tgacctcaaa ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga
    10321 ctgcatgatc atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt
    10381 tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc
    10441 cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg
    10501 tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt
    10561 gggaacgaaa atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc
    10621 gaaagatatg cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga
    10681 catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg
    10741 cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct
    10801 ggaacgctgg aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt
    10861 gtcgcctgcc tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc ttaccctccc
    10921 ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac
    10981 caaccatcct tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa
    11041 actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct
    11101 ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct
    11161 gcaaattgtt cgctttaccg actaccagac ccgctctaat gaaaaccgca ttaagctggt
    11221 gcctatcgcg cccagccgcg gcattattta cgaccgtaat ggtatccctc tggccctcaa
    11281 ccgcactatc taccagatag aaatgatgcc agagaaagtc gataacgtgc agcaaacgct
    11341 ggacgctttg cgcagcgtgg ttgatctgac cgatgacgat attgctgcat tccgaaaaga
    11401 gcgcgcacgt tcacaccgtt tcacctctat tcccgtgaaa actaacctga ccgaagtaca
    11461 agtagctcgc tttgccgtca atcagtaccg ttttccgggg gttgaagtta aaggctataa
    11521 acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat
    11581 caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac
    11641 gcatgatatc ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac
    11701 cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt
    11761 accaccgcaa gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat
    11821 tgaaacgctg ctggcgggta gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg
    11881 ggtgctggcg ctggtttcca cgcctagtta tgacccaaac ttgtttgttg acggtatctc
    11941 cagcaaagat tattccgcct tgttgaacga cccgaataca ccgctggtga accgcgccac
    12001 acagggggtt tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattgag
    12061 cgccggggtg atcacgcgca atacgacgct gtttgaccca ggctggtggc aactgccagg
    12121 ttcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag
    12181 atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga
    12241 tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc
    12301 ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc
    12361 gtggtatcag ggtgacacca ttccggttgg tatcggtcag ggttactgga cagcgacccc
    12421 aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca
    12481 tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc
    12541 gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt
    12601 tgctaaccgc cctaacggta cgacgcataa atactttgcc agcgcaccgt acaaaattgc
    12661 ggcgaaatct ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca
    12721 caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa
    12781 tccgcaagtg gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac
    12841 gctgatgcgt cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc
    12901 ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa
    12961 aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg
    13021 ctggtttaca gcgccctagt tatctggagc gccagcggtc aggatattgg catgatggag
    13081 cgtaaaatcg gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct
    13141 ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg
    13201 gcggtagatg ctttcggtgc catctctaaa ggtgctcaac gctggctgga cctcggtatt
    13261 gttcgttttc agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt
    13321 atcaaccgcg acgtttgccc accgtcgttg aagaacacag gcatcgcgct ggtgctgata
    13381 tttatgccca cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg
    13441 ctttccggtc tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca
    13501 gtagtgctgg tggcggcgtt cattcctatt ctgtggttct tcctgatgca tgattaccag
    13561 cgccagcgcg taatgatgct cctagacccg gaatcagacc cactcggcgc gggctatcac
    13621 attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcat
    13681 ggcactcagt cacaacttga atttctaccc gaacgccata ccgactttat cttcgcggta
    13741 ctggcggaag agctgggatt agtgggcatt ttgattctgc tcgctctcta cattctgctg
    13801 atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct
    13861 ggcggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt
    13921 attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc agcgctaatt
    13981 gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg
    14041 aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg
    14101 aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc
    14161 tgcggtatgt aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact
    14221 gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga
    14281 tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa
    14341 cctgacggcc tctggcgaag ctttcgatcc gacacagctg acggcggccc atccaacgct
    14401 tccgatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg
    14461 cattaatgat cgcggccctt acggcaacga ccgcgttatt tcgctttctc gcgcggcagc
    14521 tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca
    14581 ggatggttcg ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc
    14641 cctgcctgca cctcccgatt taagcggtgg cgcgggaaca agttcagtgt ctggcccgca
    14701 gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc
    14761 ggtaaccagt agcggtttcc tcggcgcacc aacgacctta gcgcctggtg tactggaagg
    14821 cagcgaaccg acgcctgcgc cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac
    14881 ctcgcctgca atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt
    14941 gcaagtcggg gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca
    15001 gaagttcggc gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg
    15061 ccccttcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca
    15121 attacagtca tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt
    15181 ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc
    15241 tatagtaggg cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    15301 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    15361 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga
    15421 tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga
    15481 acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    15541 gccaagcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    15601 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    15661 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    15721 gtgtcgccat ggctgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    15781 gctacgttaa cgccctgggc ctgaaaaaca cccacttcca gacggtacat ggtctggatg
    15841 ctgatggtca gtacagctcc gcgcgcgata tggcgctgat cggccaggcg ttgatccgtg
    15901 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    15961 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    16021 gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct
    16081 tgatctctgc ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac
    16141 tgctgacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt
    16201 tcgcctctga accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag
    16261 acatgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga
    16321 acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    16381 agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag
    16441 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    16501 aaaaattaaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa
    16561 ctcccgcctt ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac
    16621 caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc
    16681 gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta
    16741 caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa
    16801 cgccactcat atcgagcagg ttgaaatact gtatgaagaa ctgggcaaaa tcgatattgt
    16861 ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct
    16921 cctcgctgtg atatactttt cccccacttt tactcattct ccacggagat gccgttttgt
    16981 atcaggataa aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg
    17041 ctatgcatga attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg
    17101 agcactatcc ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg
    17161 gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac
    17221 aacaggtgat gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg
    17281 tgaccttgct tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc
    17341 ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac
    17401 gaattcgacg cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac
    17461 catttttacg tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat
    17521 ggaaacccga agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc
    17581 tactaaacaa tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat
    17641 ttacactggg tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat
    17701 attttcttta tcacttgcag aaatgaggtg ccacgccttg ctccgtaact caccccattg
    17761 attaacctat tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata
    17821 agattattgt aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg
    17881 atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa
    17941 tatttacaac cttacgaaat attgctctta accttctgac tatttttgaa gctgtatatg
    18001 tacataaagg gatcgttaat gcagcgaaag tgcttaatct gcccccctcg gcaatcagtc
    18061 agtctattca gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccaag
    18121 gtgtcactcc taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt
    18181 ccattcttgg cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg
    18241 cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc
    18301 actatccgca gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc
    18361 agtttcaaac cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc
    18421 atgttctgtt caccgacaat atggtgttaa tttgccgcga gggaaatcca ctactctctt
    18481 tagaagatga cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagagc
    18541 aaaatttcag cggtctgcgg cagagagttc aagaaatgtt tccggaccgg caaatcaatt
    18601 tcaccagcta caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga
    18661 ttattcccag ccgtttttat aacctgttta gccgctgctg gccgctggaa aaactgcctt
    18721 ttccgtcctt aaataaggag caaatagatt tctctatcca ctacaacaaa ttcagcctgc
    18781 gtgatcccat cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg
    18841 ttacagccag atcacagcgc agaacgataa aatggcacaa tccaacaaca attttacatt
    18901 ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt
    18961 caaaaatagt tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc
    19021 aactggaaca cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc
    19081 gatgccgata agatggccct tatcccggtt aaaaacgtgg caatagagcg cgaagccctg
    19141 ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc
    19201 atcaaagccg caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct
    19261 aacctggcgg aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt
    19321 acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat
    19381 gaaccagtga aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc
    19441 tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc actttgcgga ttgcattact
    19501 gccattcggg aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgtggt
    19561 cgtatggatc gtgctctgga tattctgact gcaacgccac cagatgtgtt caaccataac
    19621 ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg gtgcagatta caactggtcg
    19681 ctgaagctgc tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtcta
    19741 atggtgggac tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt
    19801 catggtgtga cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg
    19861 gttcaacgtt acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg
    19921 ggctttaccc atgctgcatg cggtccgttt gtccgttctt cttaccacgc cgatttgcag
    19981 gcgaaaggga tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa
    20041 cccgcttttt gaagcggttt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc
    20101 cacgtcactc tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg
    20161 ccgcagcatc atcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc
    20221 cgcccagcgt acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacta
    20281 gcagtttggt aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga
    20341 acgcgcctta aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc
    20401 tgtaacaagg tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa
    20461 gcgacggttg tttaagacgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac
    20521 ttccgccata atgagtgcag gcatttctga cgctgccgca atggtgacgc caaagggatc
    20581 tataatccgg ctttgcccga tatttttgtt cccgcactcg cccgccgcca ccatataaca
    20641 ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag tgatgctctt tgagcggccc
    20701 gcgaacccag gcggcaggaa gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc
    20761 cagctctgga aagcgtaagt cataacaggt catcagaccg accttcatcc cttccacctc
    20821 cagtaacgga gcgatttcat taccagcatc aacacggcgt gattcctgaa tggcaaatgc
    20881 atcatagaga tgcagtttgg cataacgggc gacgatgttt cctgcctgaa gtgccaccag
    20941 catattccat gcgcgccccg gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat
    21001 gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga
    21061 tttaaccgat agatctgcat catgatcatc gcgtgccagc aatgcttccg gcaggacaaa
    21121 cagcgatacg tcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt
    21181 ttcccacaca gatgtaacag caaactgtcc tgcagcaacc agcattgcca cctcctgtca
    21241 gagtcgtaaa attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca
    21301 aatagcagga ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg
    21361 gaagcgtggc gagatggctg ttaagtatgc gatttaaccc actgcatcag gcgattccgt
    21421 tggggacgct gacagcaaac ctgattgggg cattcatcat aggaatggga ttcgcatggt
    21481 tcagcaggat gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg
    21541 gcggtctaac aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggtc
    21601 gctttggctg ggcattactg aacgttttcg tcaaccttct ggggtctttt gccatgacag
    21661 cactggcttt ctggctgttt tcggcctcaa ccgcacacta aagacccaaa aaaaacccgc
    21721 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    21781 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    21841 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    21901 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    21961 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgcgtcggt taccagtaca
    22021 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    22081 ttttgatgtt acttatttta gctattgatt ttaaagaagt tactaaaact tcatttgtct
    22141 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    22201 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    22261 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    22321 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    22381 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    22441 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    22501 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    22561 tgttcaggct gttgccaggt tggtgcaata ttttctctaa acgttgtcat ccactcatct
    22621 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    22681 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    22741 tagcaaagct acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc
    22801 tgctctattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    22861 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    22921 atgcaatcaa ggaaaaatta ttttattttt taacgagata caacaatcac cttaacaaag
    22981 tatataatat atatcgtcaa cctatgctca tattagatta ttttttgagc gcacagccgc
    23041 agctaacacc attgcaatta acaaatttgc atcaatccac catcaatttg cacacattat
    23101 tatgtgataa ttgccaaccg ctaaatatgc gttttgttat ctatgtataa aaacagtaac
    23161 ttcaatgtct taatggcagt ttttcttgat tttaatcagc attcatcgcc aatttattgg
    23221 gcatattttt tccttaagct ttaggaattt tttattattt actttggggc ctggagacag
    23281 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    23341 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    23401 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    23461 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    23521 acctcggcat gatgattatg atgctgtgtg gatttgccgc ttacatgacc catatcggcg
    23581 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    23641 tgctgatgat tgccgcctat tttgtcgcct gtctgatgtc tctggccgtc tcttccgcaa
    23701 ccggtctggg tgttttgctg atggcaaccc tatttccggt gatggtaaac gttggtatca
    23761 gtcgtggcgc agctgctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    23821 caggggatgt ggtgctggcg gcgcaagctt ccgaaatgtc gctgattgac ttcgccttca
    23881 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    23941 ggcaacgtta tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa
    24001 tcaccaccac tgctcctgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    24061 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    24121 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    24181 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    24241 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    24301 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    24361 caatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    24421 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    24481 tgcaggcgtc aaaccttggc cgtacccttt cgcccgtttc tggcgtagtc gttgcggttg
    24541 ccgggatggc gaagatctcg ccgtttgaag tcgtaaaacg cacctcggta ccggtgcttg
    24601 ttggtttggt gattgttatc gttgctacag agctgatggt gccaggaaca gcagcagcgg
    24661 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    24721 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    24781 atggcggctg gcgcaatatt caagataacg tctggcagac gtgcggctga tggtcagtgc
    24841 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    24901 gtttagcgtc aagggatcaa tgccggtcgg cagttcgtct ttaggttcac cgcgagcata
    24961 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    25021 tttacgctgg cggaaacggg ttagcgtttg ccccagccgt tcataggcaa tgggcttaat
    25081 gagataatca aatacaccac aacgtacggc ttcagacacc gtttccatat cgctggctgc
    25141 agtggtaaac accacgtcgc cgggataatg cgcctgcacc agttcatgca ataaattaat
    25201 ccctctgccg tcaggaagat agttatcgag caagattagc cccggcttaa aacgctcgat
    25261 catcattcgg gcctgcgcca ggtttcccgc cagtaatatc tgactgaatc cgggaatgtg
    25321 acgaatatat tccgcatgca tctctgccag cggcgtttcg tcctcaacga tcaatagggt
    25381 taatggagct gtcattaggt ttcactttcg gaatatagat tgaaaataag gtaccgcagg
    25441 gatcattatc ttcgagagtg ataacaccac cgcagcgcgt tacgtagctg gcaatcaagt
    25501 acaacccaat gccatgttca ccgggctcgt cagcacgcgt actgaccccc tgctcaaata
    25561 ttttgtctcg tagagactct ggaacgccgc agccctgatc ggcgacttca atcaccacat
    25621 catcgccttc atcgctgagg aataattcaa cgatcttgtt tccttcatcg ctacgcaggc
    25681 tggcttcgaa ggcgttatca agtaaattgc ccacaatggc tgcaaactcg gtgctatcca
    25741 gtcctggcgg cagttgcgaa agctggctac cggggacaat gatcattttt agccccagtt
    25801 cccgggcgcg ctgcacttta ccaaaaagca gccccgccac ctagcgatcg gcaaacgcct
    25861 cgcgcaggct gtcaataagc tgttgctggg cctgagactc cccctgcacc atcgccagca
    25921 cgcgatcata ctctttcatc tgcaacagac cattgagcgt cgacatccaa ttgagatgct
    25981 cgtgtcgcaa tgtacgaagg ctctcaacgt attgttttat ttgcgtcagt tgcgcattga
    26041 gggtggaaat ttcgtcttta ctgcgaaagc tgataatggc ccccagcaaa tcatcacctg
    26101 aacgaatagc ttcccgattg gcaataacgc tcagaccgtt aaagttagcc accatatcct
    26161 gacgtttttc atcaatctgt tcggtaaaga aatcggcggg cctgaccact tcagcaatgg
    26221 gtttacccaa ccattgccgt ccgggggaac tcagccccag catctttctt gcgttacgat
    26281 tgatggcggt aatgtaaccg tgcggatcca ccgcaatcag cccttcataa accgaactaa
    26341 acagcgcctc ttgctgacgg accacgcgtg cgatttgctt tggctccatg cccatcatct
    26401 gccgacggat atgcgcggcc aggaaccacg acagcaacat cagaatccct aacagcacga
    26461 caaacacacc tgccatcggt aataaaaact cagcccgcca gctatcgatt ttactcacca
    26521 ggtagccaat cgacaccacg ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg
    26581 cgcgcatcgc catccccatt gaccctttcc cggtaatgaa gtagctctcc cctttctcca
    26641 gcgcgccctg tttggtgaac tgcataggat aaccaatttt ctccggatta ggatggtaaa
    26701 ggcggatcga gtgccggtcg ccatcaccac ataatcaaaa tcggtatctc tttgtaattt
    26761 gttagcgatg gtcgccagcc gtttgtagtc acgcgttttc accgcagaga tgacactgtc
    26821 attggaggca ataattttcg cctgattcat tgccatgtcg cgtacatgaa gcgttaaata
    26881 gtcctcaaaa ctggccgtaa aatattgcgc caaggctgca atgacaaata ttgagaacac
    26941 cagaatcagc aaaaagatac gcagcggaaa tgccagtctt tggaaaaatg caaactgttt
    27001 attctcgtta agctgcaaca ttgttttcct tgcgggttat cccgagcctc tttgacagat
    27061 gtaattaaaa tgttgaatca aaatacatag atattaatta tgatcgtggc aaattttatt
    27121 cattaaatca attaaatcac ttaaacctgc aggtcatttc gtaattacat taattaaaaa
    27181 acttaaacca ttaaataaat aaaaatttca cgccgaaata ctccttagga tgtatagcga
    27241 aaggagaaaa agatatacct cgatcacccc cctttctccc aagtgaaaat aaaaggttat
    27301 cagtttgcaa cattgaacaa cattcgttgc aaatcgataa caacatgcac cttcaggata
    27361 ctatttatta tgttcggcaa tgatattttc acccgcgtaa aacgttcaga aaataaaaaa
    27421 atggcggaaa tcgcccaatt cctgcatgaa aatgatttga gcgttgacac cacagtcgaa
    27481 gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat tgccggaaat
    27541 attattaaat gcgttgctat cagtgaatcc gttcgcggtg aaggactggc gctgacatta
    27601 gccactgaac tgataaacct cgcctatgag cggcacagca cgcatctgtt tatttatacc
    27661 aaaaccgaat acgaggcgct gttccgccag tgcggttttt ccacgctgac cagcgtaccc
    27721 ggcgtgatgg tgctgatgga aaacagcgcc acgcgactga aacgctatgc cgaatcgctg
    27781 aaaaaatttc gtcatccagg gaacaagatt ggctgcattg tgatgaacgc caatcccttt
    27841 acgaatggtc accgttatct gattcaacaa gctgcggcac agtgcgactg gttgcatctg
    27901 tttttagtca aagaagattc ttcacgcttc ccctatgaag accggctgga tctggtgtta
    27961 aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg gctccgaata catcatctcc
    28021 cgcgctacgt tcccttgcta cttcattaaa gaacagagcg tcattaacca ttgttacacc
    28081 gaaattgatc tgaagatttt ccgtcagtac ctcgctcccg cactgggtgt aactcaccgc
    28141 tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt acaaccagga tatgcgctac
    28201 tggctgaaaa cgccgactat ctccgcaccg cccatcgaac tggttgaaat tgagcggctg
    28261 cgttaccagg agatgccgat atccgcttcc cgggtacgtc aactgctggc gaaaaacgat
    28321 ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc attatttgca gaacctgctt
    28381 gagcactccc gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga aacaggtgaa
    28441 aaatgaaaat aaaccagccc gccgttgcag gcacccttga gtctggggat gtgatgatac
    28501 gcatcgcccc actcgatacg caggatatcg acctgcaaat caacagcagc gttgagaaac
    28561 agtttggcga tgcaattcgc accaccattc tggacgttct cgcccgctac aacgtgcgcg
    28621 gcgtacagct gaatgtcgat gacaaaggca cactggactg cattttacgt gcacgactgg
    28681 aagccctgct ggcacgcgcc agcggtatcc cgactctgcc atgggaggat tgccaatgat
    28741 ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt ttgtgcctgg
    28801 tgccaatgcc gcgatggtca gcaactcctt catctacccg gctgatgccc tgatgtttga
    28861 cctcgaagac tccgtggcat tacgtgaaaa agacaccgcc cgccgcatgg tttatcacgc
    28921 gctgcaacat ccgctgtatc gcgatattga aaccattgtg cgtgtcaacg cgctggattc
    28981 cgaatggggt gttaacgacc tggaagccgt cgttcgcggt ggtgcggacg ttgtgcgtct
    29041 gccgaaaacc gataccgctc aggatgttct ggatattgaa aaagagatcc tgcgtatcga
    29101 aaaagcctgt ggtcgtgaac ccggcagcac cggcctgctg gcggcgattg aatctccgct
    29161 gggcattacc cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg gtatcgccct
    29221 cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc tccccggaag gaactgaact
    29281 gctgttcgca cgctgttcca ttttgcaggc cgcgcgctct gcgggtattc aggcgttcga
    29341 taccgtctat tccgacgcta acaacgaagc cggattcctg caagaagccg cccacatcaa
    29401 acagctgggc tttgacggca aatcgctgat caacccgcgt cagattgatc tgctgcacaa
    29461 cctctacgca ccgacccaga aagaagtgga tcacgcccgc cgcgtcgtag aagccgctga
    29521 agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg tggacggtcc
    29581 ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa ctttccggca tccgcgaaga
    29641 ataaggcaat caaaatgacg cagaaaattg aacaatctca acggcaagaa cgggtagcgg
    29701 cctggaatcg tcgcgctgaa tgcgatcttg ccgctttcca gaactcacca aagcaaacct
    29761 accaggctga aaaagcgcgc gatcgcaaac tgtgtgccaa cctggaagaa gcgattcatc
    29821 gctctggttt acaggacggc atgacggttt ccttccatca cgctttccgt ggcggtgacc
    29881 tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac ctgactctgg
    29941 cctccagctc cctgagtgat tgccatgcgc cgctggtaga acacattcgc cagggcgtgg
    30001 ttacccgcat ttatacctct ggcctgcgcg gtccactggc ggaagaaatc tcccgtggtc
    30061 tgctggcgga accggtgcaa atccactctc acggcggacg tgtgcatctg gtacagagcg
    30121 gcgaactgaa tatcgacgtg gctttcctcg gcgtcccatc ctgtgatgaa ttcggtaatg
    30181 ccaacggtta caccggtaaa gcctgctgtg gctccctcgg ctatgcaatg gttgatgccg
    30241 acaacgcaaa acaggtcgtg atgcttaccg aagaactgct gccttatccg cataatccgg
    30301 caagcattga gcaagagcag gttgatttga tcgtcaaagt tgatcgcgtt ggcgatgctg
    30361 caaaaatcgg cgctggtgcg acccgtatga ccactaaccc gcgcgaactg cttattgccc
    30421 gtagcgctgc ggatgtgatt gtcaactctg gctacttcaa agaaggtttc tccatgcaaa
    30481 ccggcaccgg cggcgcttcg ctggcggtaa cccgtttcct ggaagacaaa atgcgtagcc
    30541 gcgatattcg cgccgacttc gccctcggcg gtattactgc aacgatggtt gatctgcacg
    30601 aaaaaggtct gatccgcaaa ctgctggatg tgcagagctt tgacagccat gctgcgcaat
    30661 cgctggcccg taaccccaat cacatcgaaa tcagcgccaa ccagtacgct aactggggtt
    30721 cgaaaggcgc atcggttgat cgtctcgacg tggtggtact gagcgcgctg gaaattgaca
    30781 cccagttcaa cgttaacgtg ctgaccggct ctgacggcgt actgcgtggt gcttccggtg
    30841 gtcactgcga taccgcgatt gcctctgcgc tttccatcat cgtcgcgccg ctggtacgcg
    30901 gtcgtattcc gactctggtg gataacgtgc tgacctgcat caccccgggc tccagtgtcg
    30961 atattctggt cacagaccac ggtatcgcag ttaacccggc acgtccggag ctggcagaac
    31021 gtctgcagga agcgggcatt aaagtggttt ccattgagtg gctgcgtgaa cgtgcgcgtc
    31081 tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg cgtcgttgcc gttgtgcgtt
    31141 accgcgatgg ctcggtgatc gatgttgtac atcaggtgaa ggaataagcc atgcacctgc
    31201 ttcctgaact cgccagccac catgcggtgt caattcccga gctgctcgtc agccgggatg
    31261 aaaggcaagc acggcaacac gtctggctca agcgccatcc tgttccactg gtctccttta
    31321 ccgtggttgc gcctaggccg attaaagaca gtgaggtcac acgccgaatt tttaatcatg
    31381 gcgtgacagc cttgcgtgcc ttagccgcaa aacagggctg gcaaattcag gagcaggctg
    31441 cactggtttc cgccagcggg ccggagggta tgttgagcat tgccgccccg gctcgcgacc
    31501 tcaagctcgc caccattgag cttgaacata gtcatcctct cgggcggtta tgggatatcg
    31561 atgtcctgac gcccgaaggc gaaattctct cccgccgcga ctattcactg ccgcctcgcc
    31621 gctgcctgtt gtgcgagcaa agcgcagccg tctgcgcgcg tggaaaaccc atcaactgac
    31681 cgatttactc aaccgcatgg aggcactgct gaacgatgtc gatgcctgca acgtcaacta
    31741 aaaccacaaa gcttgcgacg tcattaatcg atgagtacgc cctgctgggc tggcacgcca
    31801 tgctgactga agtcaatctg tcaccgaaac caggtctcgt ggatcgcatt aactgcggtg
    31861 cgcataaaga tatggcgctg gaagatttcc accgcagcgc gctggcgatt cagggctggc
    31921 taccccgttt cattgaattt ggtgcctgta gtgcggaaat ggcaccagaa gcggtactcc
    31981 acggattacg cccaattggt atggcttgcg aaggtgatat gttccgcgcc accgcgggcg
    32041 taaacacgca taagggcagc attttttctt tagggctact gtgtgcggca attggccgtt
    32101 tgcttcagct caaccagtcg ctaacgccaa caaccgtttg ttctacggcg gcaagtttct
    32161 gccgtggcct gaccgatcgc gaactgcgta ccaataattc acaactgacg gcaggtcaac
    32221 ggttgtacca gcagcttgcc cttaccggcg cacgcggtga agccgaagcg ggttatccac
    32281 tggtgatcaa ccacgccttg ccgcattacc tcactctgct ggatcagggg ttagatcctg
    32341 aactggcatt gctcgatacc ttgctcctac tgatggcgat caacggcgat accaacgttg
    32401 cttcgcgcgg tggcgagggg gcctgcgctg gctacagcgc gaggcgcaaa cattattgca
    32461 aaaagggggc attcgaaccc ccgccgatct cgattatctc cggcagttcg acagggagtg
    32521 tatcgaacga aatctcagtc taggcggcag tgccgacctg ctgatcctta cctggttttt
    32581 agcacagatt taattattta agcacttgat aaatttggaa atattaattt tcggagaacc
    32641 cgtatgtctt tagcaaaaga taatatatgg aaactattgg ccccactggt ggtgatgggt
    32701 gtcatgtttc ttatccctgt ccccaacggt atgccaccac aggcatggca ttacttcgct
    32761 gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa ttccggcaac agcgatcagt
    32821 tttattgcgg ttactatttg cgttattggc agtaattacc tgctctttga tgccaaagaa
    32881 ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga aatggggtct ggctggtttt
    32941 tccagcacca cggtatggct ggtatttggc gcatttattt ttgcattagg gtatgaagtt
    33001 tccgggttag gtcgtcgcat tgcccttttc ctggtgaaat tcatgggcaa acgcacgctg
    33061 acgttgggtt atgcgattgt cattatcgac attctgctgg cgccgtttac accgtccaac
    33121 accgcgcgta ccgggggtac ggtttttccg gtcattaaaa acctgccgcc gttgtttaaa
    33181 tcattcccca acgatccgtc cgcgcgtcgt attggcggct atttgatgtg gatgatggtc
    33241 attagtacca gtctgagttc gtccatgttt gtcaccggtg cggcaccaaa cgtgctgggt
    33301 ctggagttcg tcagcaaaat tgccggtatc cagattagct ggttgcagtg gttcctctgc
    33361 ttcctgccgg ttggggttat cttgcttatc attgcgccgt ggctttccta cgtgctgtac
    33421 aaaccggaaa tcacacacag tgaagaagtg gcaacctggg cgggtgatga actgaaaacc
    33481 atgggtgcgc tgacacgcag agagtggaca ctgattggcc ttgtattgct cagcttaggt
    33541 ttgtgggttt ttggcagtga agtcattaat gctactgcag ttggtctgct ggcagtttcg
    33601 ctaatgctgg ccctgcacgt tgtgccttgg aaagatatta cccgctataa cagcgcatgg
    33661 aacacgctgg tcaacctggc aactctggtt gtgatggcta acggcctgac tcgttctggc
    33721 tttattgact ggttcgccgg taccatgagt acgcacctgg aaggattctc accaaacgca
    33781 acggtgattg tactggttct ggtgttctac tttgcacact acctgtttgc cagcctgtct
    33841 gcgcacaccg caaccatgct gccggttatt ctggccgtcg gtaaaggtat tccgggcgta
    33901 ccaatggaac aactgtgtat cctgctggtg ctgtctatcg gtatcatggg ctgtctgacg
    33961 ccctatgcaa ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa atcaaaagat
    34021 tactggcgtc ttggcgcaat cttcggggtg atttatatct ctatgttgct gttggttggc
    34081 tggccgattc tcgccatgtg gaactaatac gttcgccaaa cgctgaataa atcatcacgc
    34141 ccgccaggta agcccacctg gcgggcgtga tgatttaata gatagtattc agtgtatgta
    34201 tgagttccac acccattatg aaagcattct ggcgtaacgc cgcgttgctc gcggtttctc
    34261 tgcttccctt ctcttctgcc aacgccgtag cgttgcaggc aaaacagtat ggcgattttg
    34321 atcgctatgt cctggccctc tcctggcaaa ccggattttg ccagagtcaa cacgatcgaa
    34381 atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac gaccaacaaa gctgattttc
    34441 tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt tgctgcccgt ggtgttgatg
    34501 aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat cccgaatcta ccagaagcgc
    34561 acgccagccg gatgtgttca tcgccggaaa ccgggttatc actggaaacg gc
//
LOCUS       DAJTNN010000023        34546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTNN010000023 DAJTNN010000000
VERSION     DAJTNN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.023"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            113..719
                     /locus_tag="OO150_000992"
                     /pseudo
     CDS             113..719
                     /locus_tag="OO150_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            822..1324
                     /locus_tag="OO150_000993"
                     /pseudo
     CDS             822..1324
                     /locus_tag="OO150_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1391..2848)
                     /gene="nuoN"
                     /locus_tag="OO150_000994"
     CDS             complement(1391..2848)
                     /gene="nuoN"
                     /locus_tag="OO150_000994"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5515217.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQSLISIVRLAMPLM"
     gene            complement(2855..4384)
                     /gene="nuoM"
                     /locus_tag="OO150_000995"
     CDS             complement(2855..4384)
                     /gene="nuoM"
                     /locus_tag="OO150_000995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5515218.1"
                     /translation="MLLPWLILIPFIGGFLCWQTEHFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4548..6389)
                     /gene="nuoL"
                     /locus_tag="OO150_000996"
     CDS             complement(4548..6389)
                     /gene="nuoL"
                     /locus_tag="OO150_000996"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5515219.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6386..6688)
                     /gene="nuoK"
                     /locus_tag="OO150_000997"
     CDS             complement(6386..6688)
                     /gene="nuoK"
                     /locus_tag="OO150_000997"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5515220.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6685..7239)
                     /gene="nuoJ"
                     /locus_tag="OO150_000998"
     CDS             complement(6685..7239)
                     /gene="nuoJ"
                     /locus_tag="OO150_000998"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5515221.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7251..7793)
                     /gene="nuoI"
                     /locus_tag="OO150_000999"
     CDS             complement(7251..7793)
                     /gene="nuoI"
                     /locus_tag="OO150_000999"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5515222.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7808..8785)
                     /gene="nuoH"
                     /locus_tag="OO150_001000"
     CDS             complement(7808..8785)
                     /gene="nuoH"
                     /locus_tag="OO150_001000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5515223.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8782..11508)
                     /gene="nuoG"
                     /locus_tag="OO150_001001"
     CDS             complement(8782..11508)
                     /gene="nuoG"
                     /locus_tag="OO150_001001"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5515224.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11561..12898)
                     /gene="nuoF"
                     /locus_tag="OO150_001002"
     CDS             complement(11561..12898)
                     /gene="nuoF"
                     /locus_tag="OO150_001002"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5515225.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12895..13395)
                     /gene="nuoE"
                     /locus_tag="OO150_001003"
     CDS             complement(12895..13395)
                     /gene="nuoE"
                     /locus_tag="OO150_001003"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5515226.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13398..15200)
                     /gene="nuoC"
                     /locus_tag="OO150_001004"
     CDS             complement(13398..15200)
                     /gene="nuoC"
                     /locus_tag="OO150_001004"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5515227.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15294..15956)
                     /gene="nuoB"
                     /locus_tag="OO150_001005"
     CDS             complement(15294..15956)
                     /gene="nuoB"
                     /locus_tag="OO150_001005"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5515228.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15972..16415)
                     /gene="nuoA"
                     /locus_tag="OO150_001006"
     CDS             complement(15972..16415)
                     /gene="nuoA"
                     /locus_tag="OO150_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5515229.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17046..17984)
                     /gene="lrhA"
                     /locus_tag="OO150_001007"
     CDS             complement(17046..17984)
                     /gene="lrhA"
                     /locus_tag="OO150_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5515230.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18903..20120
                     /gene="alaA"
                     /locus_tag="OO150_001008"
     CDS             18903..20120
                     /gene="alaA"
                     /locus_tag="OO150_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5515231.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20204..20803
                     /gene="yfbR"
                     /locus_tag="OO150_001009"
     CDS             20204..20803
                     /gene="yfbR"
                     /locus_tag="OO150_001009"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5515232.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20862..22694)
                     /locus_tag="OO150_001010"
     CDS             complement(20862..22694)
                     /locus_tag="OO150_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5515233.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22781..23431)
                     /gene="hxpA"
                     /locus_tag="OO150_001011"
     CDS             complement(22781..23431)
                     /gene="hxpA"
                     /locus_tag="OO150_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5515234.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPKVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(23442..23936)
                     /locus_tag="OO150_001012"
     CDS             complement(23442..23936)
                     /locus_tag="OO150_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5515235.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACLRQYHLSANEINQ
                     IINA"
     gene            complement(24020..24475)
                     /locus_tag="OO150_001013"
     CDS             complement(24020..24475)
                     /locus_tag="OO150_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5515236.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24813..26015
                     /gene="ackA"
                     /locus_tag="OO150_001014"
     CDS             24813..26015
                     /gene="ackA"
                     /locus_tag="OO150_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5515237.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26090..28234
                     /gene="pta"
                     /locus_tag="OO150_001015"
     CDS             26090..28234
                     /gene="pta"
                     /locus_tag="OO150_001015"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5515238.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNQEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28424..29944
                     /gene="yfcC"
                     /locus_tag="OO150_001016"
     CDS             28424..29944
                     /gene="yfcC"
                     /locus_tag="OO150_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5515239.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVSRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29977..30519)
                     /gene="yfcD"
                     /locus_tag="OO150_001017"
     CDS             complement(29977..30519)
                     /gene="yfcD"
                     /locus_tag="OO150_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5515240.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30577..31128)
                     /gene="yfcE"
                     /locus_tag="OO150_001018"
     CDS             complement(30577..31128)
                     /gene="yfcE"
                     /locus_tag="OO150_001018"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5515241.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31184..31816)
                     /gene="yfcF"
                     /locus_tag="OO150_001019"
     CDS             complement(31184..31816)
                     /gene="yfcF"
                     /locus_tag="OO150_001019"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5515242.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASVAMAEHLLALGQPNLFGEW
                     CIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31952..32599
                     /gene="yfcG"
                     /locus_tag="OO150_001020"
     CDS             31952..32599
                     /gene="yfcG"
                     /locus_tag="OO150_001020"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5515243.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGELGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32656..33018
                     /gene="folX"
                     /locus_tag="OO150_001021"
     CDS             32656..33018
                     /gene="folX"
                     /locus_tag="OO150_001021"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5515244.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33039..33932
                     /locus_tag="OO150_001022"
     CDS             33039..33932
                     /locus_tag="OO150_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5515245.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33980..>34546)
                     /gene="rpnB"
                     /locus_tag="OO150_001023"
     CDS             complement(33980..>34546)
                     /gene="rpnB"
                     /locus_tag="OO150_001023"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5515246.1"
                     /translation="AGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSS
                     AFPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLK
                     ALFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQ
                     EMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8661 a         9396 c         8644 g         7845 t
ORIGIN      
        1 ttgtatagat ctaacaaccg taataaaaca aatactcata actcattata aataatgata
       61 aaataacgtc ttttattgat aatttatata tgggaaaaat tccttttgtt atattgtttc
      121 tgatttcact ccctctgatt atttttacca accctgggtc actgcattac caccaacgcc
      181 ccgacacgtc agtcctgagc aattagaaaa aacagttcgt tatcttacac aaactgtgca
      241 tccacgtaat gccgacaata ttgataatct gaataggtcc gtagagtata taaaagagtt
      301 ctttgtcagt agcggtgcca gagttaccgc gcagggcgtc cccgattggc tattacgact
      361 ctgcgcctgg tagccaggat tacctttacc cgcaatgtcc tggctttatc ccgatcgggg
      421 ggactttatt gcctggtcgg cagaatacag gatatcaacg ccgctcgtca ggtaaaaacg
      481 gcattgttat catcccggga tttatctgtt tattctatga atgccccatg gtttattccc
      541 ggtattgatt tctctgacca cctgaattat tggcaacacg atattcccgc cgtaatgatt
      601 actgacaccg ctttttatcg taataaacaa taccacttgc ccggtgatac agcagacaga
      661 ttgaattatc agaaaatggc tcagatggtg ttataacctt gttacacaac agtaaataat
      721 aaaaatttct cgaggcgggt ccagaaacag cgacatgtac attttatttt ccctcgtctg
      781 ttttaagatg cgaaacataa aattcaatgg aggaaaaata aatgggaatg attggttatt
      841 ttgcagaaat tgactcagaa aagattaacc aatctgcttg agtctactga aaaacctttg
      901 atggataata tacatgacac gctttcagga ttacgccgac tggatattga taaacgctgg
      961 gattttttac actttgggct gacaggcacc cctgcttttg atcccgcgaa gaatgatccg
     1021 ctgagtcgtg ccgtgcttgg tgaacacagc ctcgaagatg gcatagatgg cttcctggga
     1081 ttgacctgga atcaggaatt agccgcgaca atcgaccgac tggaatcact ggatcgaagt
     1141 aagctgcgta aacagttttc cattaagcga ctatgaaatg gagatttatc cgggagtgac
     1201 cttttcagaa gagttagagg ggcaactctt tgcctcgatc atgcttgata tggaaaagct
     1261 gattagcgcg tatcgacgta tgctgcgaca agggaatcat gcgctgacgg ttattgttgg
     1321 gtgatggtgt cagagcgcca aaaaaaaccg ccgaacatgt cggcggtttt ctgacttaca
     1381 aactaacaga ttacatcagc ggcattgcta accgcacaat actaatcagc gattgtggcc
     1441 atacacccag caccagtacc aacagtgcag aaatcagcac cacgataccg cccgcgctgt
     1501 actgccagtt tgacggtgca tcgcgacccg gttgttccgg tgcgtgcagg tacaggctca
     1561 ccgccacgcg caggtagtag tagaggccga ttgccgaacc gacaaccacg gcacccacca
     1621 gccaccacaa gtgtgcctgg acaccgaccg ccagcacgta gaacttaccg ataaagccca
     1681 gcgtcatcgg gataccggcc agagacagca tcatcaccgt catcactgcc gcgaggatcg
     1741 gacgatgcca gaacagaccg cggtaagaga acagggaatc agcatccggg ccacgatacg
     1801 ggctggacat caggctgacc acgccgaacg cgccgaggct gctgaacaga taaccggcca
     1861 ggtaaacccc taccgcttcc atcgacatct cgccggtttg cagcgcaatc agcgccacca
     1921 gcagatagcc gaggtgagag atagatgagt aaccgagcag acgtttgatg ttggtctggc
     1981 tcaacgccat caggttaccg aagatgatgg aggcaaaggc gataatcgcc agtactacgc
     2041 gaatcgcttc gctgtcaccc accggtgcat agaggaacag acgcatcacc acaccgaaga
     2101 tagcgatttt gctcgccgtc gccaggaaag tggaaaccgg cgcaggcgcg ccttggtata
     2161 cgtctggcgt ccacaggtgg aacggcacca gagagagttt gaagccgagg ccaacaatca
     2221 tcatgccgaa acctgccagc aacagcggct cgttgagcat accgtcaccg aggtttttac
     2281 ccagcgcgac aaacgacagg tcgccagact gcgcatacac cagcgccata ccaaacagca
     2341 ggaaagaaga cgctgcggca gaaaggatgg tgtatttgat actggcttcc agcgaacgtt
     2401 tctggcggaa agcgtaaccg accaggccaa acagcggcaa agagatcagt tcaataccga
     2461 ggaacagaga cgccagatgg tttgcattcg ccagcaggat cccgcccagc gcggcaatta
     2521 acaccaacag gtagaactca tccttgttgt cgttatagcc ttcaagccac gggtaggcga
     2581 aagtacaggt ggcgaggctc gccaacaata ccagcccggt gtaaagcatg gcgaaaccat
     2641 caacgcgcat cagcggcgta acgtccatag cgcccgcctg gccaacaaac cagagcgaaa
     2701 ccagcgccgc gttaagccca ataaccgaga gcgtagcgtt gaggaaatga ttgcgtcgcc
     2761 acgcaatgga gagcatcaca accaccaccg ttaagccgac gatcagcaac ggtagcagtg
     2821 cgatcaggtt ttgtggagtt attgtcatgg cgatttacgg ccttgtagta gtaacggaat
     2881 taacaaacca ctgctggata ttgccaatcg cggagtgcga ggtatccaga atcggctgcg
     2941 gatagaagcc cagcagtacc agcagcacca ccagcaacag gatcataaac agctcacgca
     3001 gcgacatccc tggcagttcc tggctggcaa tctggctttt cgctttaccg aagtaagcgc
     3061 gatgcaacat cgccagcgaa taaacagatg caaagaccag cccaaaggta gagataacgg
     3121 taatcaccgg gacaacctgg aagctgccga acagaatcat aaattcgccg acgaagttac
     3181 cggtgccagg catcccaagc gttgccaccg caaagaacag cgacaatgct ggcagccatt
     3241 tcatcttgct ccacagaccg cccatcatgc gcatgtcgcg ggtatggata cgttcataaa
     3301 gctgaccaca aagaataaac agacccgccg ccgacaaacc gtgcgcaatc atctggatta
     3361 ccgcgccctg gtaggccaac tggctgccgg tgtagatagc aatcagcacg aagcccatgt
     3421 gggaaaccga ggtgtaggcg atcagacgtt tgatatcggt ctgggcgaag gccatccacg
     3481 caccgtagaa gatgccgata acacccagcc acatagcaat tggcgcgaac tctgccgacg
     3541 cgttcgggaa cagcggcagg gagaaacgca gcaaaccgta agcggcagtt ttcagcaaga
     3601 tccccgcgag atcaacggaa ccggcggtcg gagcctggga gtgcgcatcc ggcagccagc
     3661 catgcagcgg aaccaccggc attttgactg cgaaggcgat gaagaagccc agcattaaca
     3721 ggtattccac accactggac attggcgtat tcagcagctc ttcatagttg aaggtccaga
     3781 cgccggtcgc attgtagtga acaaaaacca gcgccaggat ggcaatcaac atcaccaggc
     3841 cactcgcctg ggtgtaaatg aagaacttgg ttgccgccgt gatacgcgtt ttaccgtcag
     3901 aggctttatg cccccacagt gcgatcagga agtacatcgg caccagcatc atttcccaga
     3961 agaagaagaa caggaacatg tcgatggcaa ggaacacgcc gataacgccg cccaggatcc
     4021 acatcaggtt gaggtggaag aagccctgat atttttcgat ctctttccac gaacagagta
     4081 ccgccagcac accgagcaga ccggtcagca cgaccatcag cagcgacaga ccgtcaatgg
     4141 cgagatgaat agagatacca aaacgcggga tccacggcat gtcgaattca gactgccact
     4201 gcggaattcc ggcggattgc gtcagtgaat aaccgccctg caaccacagt tgcagcgaca
     4261 gcgccagcgt caatcccatg gtgatcagcg cgatccagcg tggcaccttg acgtcaaagt
     4321 gttcggtctg ccagcacagg aagccgccaa taaagggaat taatattagc cagggtagta
     4381 acatggcgat ctttattcct tgtaaaagtc ccgtcaggac cggattttca acgaattctc
     4441 acgacagaaa attctcttcg gactgggggt agtgtcggat gcgccgcgaa ggcagcttat
     4501 ccgacctacg ttcttatatg cccaggagca atcccacaat ccttaactca acgcagtacc
     4561 atcaacagtg ccagcaccac gaccgcaccg atgctcatgg atgccacata ccagcgcaga
     4621 taaccgttct cgcttaacag caggccttta cctgcaaagc gggaaagaac cgccggaatg
     4681 ttcatcattg agttcagcgg atcgcgtttc agcaaccagg caatacccag aaacggcttg
     4741 acgaacactt tgtcgtacag ccagtcaaag ccccaggcgt tgtaccacca ggtgcccagc
     4801 agacggcccg gcgcactgtt ggcaatggag gtcaccagag tacgtttacc ctgccacagc
     4861 caggctgcca gcagaatgcc gaccaccgcg accacgccag aggtaatttc cagggtcaac
     4921 atgctgccgt gcgccagttc cgtcgtttgc ggaagcacgc tctgcagcgg cggtacaatc
     4981 agtgcgccaa cgaaggtgga aaggatcagc agcacaatca gcggcaggct gtgagttacc
     5041 cctttcacgg cgtgagcgtg aatttgttct tttccgtgga agacgatgaa aatcatacgg
     5101 aaggtgtaga gcgaggtcat aaacgcaccg accagacctg ccaccatcag attgatatga
     5161 ccattcgcca tcgcacccgc gaggatctca tccttactga agaagcccgc agtgaccagc
     5221 ggtagtgccg acagtgctgc gccgcccacc aggaagcaga aataaaccag cggaatagat
     5281 ttacgcagac cgcccatctt gaagatgttc tgttcgtgat ggcaggccag aatgacggaa
     5341 ccggatgcca ggaacagcag cgctttaaag aacgcgtgag tcatcaagtg gaaaatcgcc
     5401 gcatcccatg cctgcacgcc aagcgcgagg aacatgtagc caatctggct catggtagag
     5461 taagcgagaa cacgtttgat gtcggtctgt accagcgcgg caaaaccggc cagcagcagc
     5521 gtaaccgccc cgacaatgcc caccagatgc agaatttccg gcgtcatcag gaacaggccg
     5581 tgggtacggg cgatcaggta gacacccgcg gttaccatgg ttgcggcgtg gatcagcgcg
     5641 gagacaggcg tcgggcccgc catcgcgtcg gcaagccatg tctgcaacgg caactgcgca
     5701 gatttaccga ccgcaccgcc cagcagcatc agcgtcgccc acatcagcat gttattgccg
     5761 tcagcaaagt gcgctggtgc cagttccacc atttcgcgga agttcagggt gcccagttcg
     5821 ttgtaaagaa tgaacagcgc gaaagcgagg aacacgtcac ccacacgggt cacgacgaac
     5881 gctttcattg ccgctgcgcc attcttcgga tcggtgtaat agaacccgat cagcagatag
     5941 gagcacaggc ccacgccttc ccagccgagg tacatcagca gcaggttatc ggcgagaacc
     6001 agaactacca tgctggcgat gaacaggttg gtgtaggcga agaagcgaga gtagccctct
     6061 tcaccgcgca tgtaccagga ggcgtacatg tgaataagga aacccacacc agtgaccacc
     6121 gagagcatgg tcagcgacag gccgtccagc accaggttaa aaccgatgtt aaagtcgcct
     6181 accgacatcc acgtccacag cggctggctg tatgcctgct cgccgttagc gaagaaatca
     6241 acgccgataa aggcggttac cagcgccgcc aggcccacag agccaacgcc gacgatcgcc
     6301 gaaacgtttt cagaccagcg cccacgggag aacgccagca ggacgaagcc aatcaatggc
     6361 aaaataatgg ttaaggcaag catgttcatc cgcgcatctc acttactgaa tcgatgttca
     6421 ggttctggcg acgacggtga agttgcagca gcagcgcaag gccgatactc gcttctgccg
     6481 ccgcgaggct gatggcgaga atgtacatca cctgaccgtc ggtctgaccc cagtagcttc
     6541 cggcgaccac aaaggccagc gcggaggcgt taatcatgat ttccagacca atcagcataa
     6601 acagcagatt gcgacggata accagaccgg ttaagccaag aacgaataag attgccgaga
     6661 ggatcagtcc atgttgtaag gggatcatgc gtgctcctcc gtttttcttt tcgcgctgtc
     6721 gtctttacga ttgctcagca cttcacccgc acgctcttca cgaccgacgt ggaaggccac
     6781 aaccagaccc gcgagcagca gcatagaagc cagttccacc gccagtacgt aaggcccgaa
     6841 cagcgtaata cccactgctt tagcactgat tggcgtaccg tcgataccct gatcattaac
     6901 accaaggatg gcgtaaacaa tcaccaccag catgatggcc gacaaaattg ccggaccaat
     6961 ccacacctgc ggtttcagcc actggcgttc ctgttcgatt tctgaaccgc ccaggttgag
     7021 catcatcacc acgaacacga acagcaccat aatggcaccc gcgtagacga taatttccag
     7081 cgcaccggcg aagtaagcgc ccagtgagaa gaacaccccg gaaatcgcca gcagcgaaat
     7141 aatcaggtac agcagtgcgt gtaccggatt ggtatgggtg atcactcgca aggtcgcaag
     7201 tatggctatc aggccacaga tataaaaagc gaactccatt gcccctctcc ttacggtaac
     7261 aggctcttga cgtcgatagg cttggcttcg ttctctgctt cgcccttatc tttgccgtcg
     7321 attgccatac ctgccatccg gtagaagtta tattccgggt atttgcccgg accggagatc
     7381 agcagatcct ctttttcgta aaccagatcc tggcgcttgt attcccccat ttcgaaatcc
     7441 ggggttaact gaatcgccgt ggtcggacag gcttcttcgc acagaccaca gaaaatgcag
     7501 cgtgagaagt tgatgcggaa aaattccggg taccagcgac cgtctttggt ttctgctttt
     7561 tgcagcgaga tacagccgac cgggcaggct accgcgcaga ggttacaggc tacgcaacgc
     7621 tcttcgccgt ccgggtcgcg ggtcagaacg atacgaccac gataacgggg cggcagatag
     7681 accggctctt ccgggtacat tcgcgtttcg cgtttggcga acgcgtgcag gccgatcatc
     7741 cagatactac gaacctgggt gccgaaacct actaacaatt cttttaaggt catggtctta
     7801 ttgcccctta ttgcgcctgc cagagaatga cagccgccgt taccagcaag ttgatcagcg
     7861 tcagcggcag gcagattttc cagccgaagg acattacctg gtcataacgc ggacgcggta
     7921 acgacgcacg aatcaaaatg aacatcatca taaagaacgc ggttttcaac gcgaaccaga
     7981 tgaatggcgg taacaacggg ccttgccagc caccgaagaa cagcgtcacc atcaatgcag
     8041 agatggtcac aatcccgatg tactcaccca cgaagaacag accgaacttc ataccggaat
     8101 attcaatgtg gtaaccatcc gccagttcct gctcggcttc cggctggtca aacgggtgac
     8161 ggtgacatac cgccacgccc gcgatggcaa aggtaataaa accaaagaat tgcgggataa
     8221 cgttccacac atgcgcctgg ctgttgacga tgtcggtcat gttgaatgaa ccggcctgcg
     8281 ccaccacgcc catcagggaa agcccgagga acacttcgta gctcagggtc tgcgcagaag
     8341 cacgcatcgc acccagcaac gagtatttgt tgttacttga ccagcccgca aacagcaccg
     8401 cgtaaaccgc cagacctgcc atcatcagga agaacaaaat cccgatgttc aggtcggcaa
     8461 ccacccaacc cggactgact ggcacaatcg caaaggccag cagcagcgag gtaaaggcaa
     8521 tcatcggtgc cagggtaaag atgacgcgat ccgagaattt cgggatccag tcttctttaa
     8581 agaacatttt gatcatgtcc gcaaccagct ggagcgaacc gccccagcca acacggttag
     8641 gtccataacg gttctggaac agacccagca ggcgacgttc gccaaagctc atgaatgccc
     8701 cacaggtgac aaccaccagc aggatcacca ccgctttgag gatggtcagc aggatctcaa
     8761 tcagttccgg tgatatccaa ctcattgttg tgcctccttg agatcctcaa gatgcgcgcc
     8821 agccagcacc ggagcaatgc cggacatacc catcggcaag cccacctgcc ctgccgtcag
     8881 tccttcggcg atttcaaccg gcagcgtgac cgtgttgcca tcgtaactaa aggagacgcg
     8941 tgtacctgcg ttcacaccca acttcgcggc atccgctggg ttgagtttga tgtacggctg
     9001 cggcatacgg ctctggaaga ccggagcacg ctgtgacaat tcatcgctgc caaacaggtg
     9061 gtaatacggc gcaatacgcc atttcccgtc ctgcggctgg aagcgtgccg gtacgctggt
     9121 gaagtaatcc agaccattct cgctggtttc aaacagacgc acgcccggat cgccaaagcg
     9181 cagtttgccg cccacttcgt cctggaattt gttccacgcc tgcggggagt tccagcccgg
     9241 tgcccaggca aacggcactt gcgaacggtg cgcggtcggc tggttgttac cttccatcga
     9301 gaaggtgaac atggtgtcaa tatcctgcgg ctgacgcggc tcatgaacgc tgatattggc
     9361 gcgcatggcg gtacgaccgc tgtaacggtg cggttcacgg gccagtttct gcccacgaat
     9421 acggaatgtc gcatccggcg cagcatcttt gatacctgcc agttccggga ttttcgccac
     9481 aacagcgtca atcacatggt cgagctgcgt ccagtccact tcacggctca gcagggtgct
     9541 gtgcagcgag tgcagccagc gccagctttc cagcatgaca gttttgctgt cgtaataagc
     9601 cggatcgtaa acctggaaga aacgttgggc gcgaccttca ttattaatta ccgtaccgtc
     9661 gctttcagca aagctggcgg cggaaagtac cagatgggcg ttttccatga tcgctgtacg
     9721 ctgatggtca accaccatca ccagcggtgc tttagccagc gcagcattca cgcgggtagc
     9781 agaagcgtga cgatgcagat cgttttccaa caccaccacc gcgtcggcgc gtccggtttc
     9841 cagttcggtt aacgcttctt caagcgaacc gccacccata atgcccagcc ccatgctgtt
     9901 gacggaacgg gcaatcatgg tgataccgac gtcagcgccg cgacctttca gggctttcgc
     9961 gacgttagcc gccgcctgaa tcacctctaa gctaccggcg ttcgtcccgg agataatcaa
    10021 cggtttcttc gcacctgcca gtgcctgcac gatgacgtcg attttgcttt gcagctcagg
    10081 ttcgataccg tcaaccgctg gtgcagagtt atccagcgca tgggcgatgg caaaacctaa
    10141 acgcgcctga tcttcaaccg gtgcgcggta agtccacgcc gcgatatcat ccagacgggt
    10201 gtcatcaacg ttggtaacaa acagcggatg cttcgcacgt tgaccgatgt taaggattgc
    10261 cgcaatctgc cagtcagcca ctttctgtgc tgccgccatt tcgcgcgctt tacctttcac
    10321 cgcctgacgc actgccagcg cgacgcgcgc gccggtctgg gtaacgtctt cacccagcac
    10381 cagtaccgca tcgtaagatt cgatttcgcg cagagccgga gtataaatgc cgccttcacg
    10441 cagcactttc agcgccagtt gcagacgttc ctgctcaccg tgagcgatac cggtgtagaa
    10501 gttttcttcg cccaccagtt cacgcagtgc aaagttgctt tccacgctgg cacgcggaga
    10561 accaataccg atcactttct tcgactgacg cagaatatct gccgcgccct gcattgcctg
    10621 ttcggcgttg agggtaatga aatcatcgcc acgacgctgt actggctgac gcggacgatc
    10681 cttcaggttg acgtaaccgt aaccgaaacg accacggtcg cagaggaagt agtggtttac
    10741 cgtaccgttg taacggtttt cgatacgacg cagttcgccg taacgttcac cggggctgat
    10801 gttacagccg atggaacatt gctggcagat gctcggcgca aactgcatat cccatttacg
    10861 gttgtaacgc tcggagtgcg ttttgtcggt aaatacgccg gtcgggcaaa tttcgaccag
    10921 gttaccggaa aattcgcttt ccagcgtgcc gtcttccggg cgaccgaagt agacgttgtc
    10981 gtgcgcgcca taaacgccca gatctgtacc gtcagcgtaa tctttgtagt aacgcacaca
    11041 gcggtagcag gcgatgcagc ggttcatttc gtgagatatg aatggcccca aatcctgatt
    11101 acggtgggta cgtttggtga aacggtagcg acggaagctg tgtccggtca tcacggtcat
    11161 atcctgaaga tggcagttac cgccttcttc acataccgga cagtcgtgcg ggtggttggt
    11221 cattaaccac tcgaccacac tttcacggaa ctgtttcgct tcttcgtcgt caatggaaat
    11281 aaaggtgcca tcggaagccg gtgtcataca ggacatcacc aggcgaccac gcgtgtcttc
    11341 cgcgttttgg tattgcttca ccgcacactg gcggcaagca ccgacgcttc ccagcgccgg
    11401 atgccagcaa aagtaaggaa tatcaaggcc cagagacaga caagcttcca gcaggttgtc
    11461 cgctccgttg acctcgtatt ctttgccgtc tacatgaatt gtagccatta gcatgcttcc
    11521 agttttctcg gtcagggacc gagcgttaat cgaaattcgg ttaccagcgc tctttcagca
    11581 ggttcggctg aatcccatta atcaaatggg tattgctgaa cggctgtttg attcccgcct
    11641 caaattcttc gcggaaatat ttgatggcgc tctgtaacgg ctccactgca ccaggtgcgt
    11701 gggcacagaa agttttaccc gggcctaaga atcgacacag ttgctcaagt gtttcaatat
    11761 cgcccggctg gccttcgcca cgctccagcg cacgcagaat tttcacgctc cacggcagac
    11821 cgtcgcggca cggcgtacac cagccgcagg actcacgggc gaaaaactct tccaggttac
    11881 gcaccagcga caccatgttg atctcatggt caaccgccat cgccagcgcc gtacccagac
    11941 ggctgcccgc tttaccgata ctttcgaatt ccatcggcag atcaaggtgc gcttcggtca
    12001 ggaagtcagt ccccgcgccg cctggctgcc aggctttaaa tttcagacca tcacgcatac
    12061 caccggcgta atcttcgagg atctcgcgtg cggtggtgcc gaacggcagt tcccacagtc
    12121 ccggattttt cacccgaccg gagaagccca tcagcttggt gccagcatct ttacttttcg
    12181 agatgttctg ataccactcc acgccgttag cgaggatcgc cggaacgtta cacagggttt
    12241 cgacgttgtt gacacaggtc ggtttgcccc atacgccgga ggttgccggg aaggggggtt
    12301 tcgagcgtgg gttagcacga cgcccttcta gggagttgat taacgctgtt tcttccccgc
    12361 agatgtagcg ccctgccccg gtatggacga acagttcgaa gtcgaaacct gttcccataa
    12421 tgtttttgcc aagcaagccc gcttcagtgg cttcggcaat ggcgcggcgc agatttactg
    12481 ccgcttcgat atattcgcca cgcaggaaga tgtagccacg gtaagctttc agcgcaaacg
    12541 cggagatgag cataccttcc accagcaggt gcggcagttg ctccatcaac aggcggtctt
    12601 tataggtgcc cggctccatt tcatcggcgt tacacagcag gtaacggatg ttcatggatt
    12661 cgtctttcgg catcaggctc cacttcaggc cagtagaaaa gcccgctccg ccgcgccctt
    12721 tcagaccagc gtcttttacc tgattaacga tttcgtccgg agacagcccg gtcagcgcct
    12781 tacgcgcccc ttcgtaaccg tttttgctgc ggtattcgtc cagccacact ggctgtttgt
    12841 catcgcgcag acgccaggtc agcggatgcg tttcgggagt acggataatg tttttcattt
    12901 ataccgctcc agcagttcag ggatcgcttc cggggtcaga tgcgcgtgag tgtcctcatc
    12961 gatcatcatg tttggccctt tatcacagtt ccccaggcag caagttggca gcagcgtaaa
    13021 gcggccatca aatgtcgttt gccctggttt gatgttcagc tttttctcga gcgccgcctg
    13081 aataccctga tacccgttga tatgacagac cacgctgtcg caataacgga tcacatggcg
    13141 accaaccggc tggcggaaga tctgactgta gaacgttgcc acaccttcga cgtcgcttgc
    13201 cggaataccc agcacatcgg cgatcgcgtg gatcgcacca tccggcaccc agccacgctg
    13261 cttctgaacg attttcagcg cttcaatgga cgccgcacgc gggtcttcgt agtggtgcat
    13321 ctcgtgctca atcgcttcac gctctgccgc actcagctca aaagcctcgg tttgtggttg
    13381 ttgattctcg tgcataatta gcggtccaca tctgacataa caaaatcgat actgcccaga
    13441 taaacaatca ggtcagacac caggctgccg cggatcgccg ccggaatttg ctgcaaatgc
    13501 gcataactcg gcgtgcggat acgagtgcga taactcatgg tgctaccgtc gctggtcagg
    13561 tagtaactgt tgatcccctt ggtcgcctca atcatctgga aagattcatt ggcaggcatc
    13621 accggccccc acgacacttg caggaagtgg gtgatcaggg tttcgatatg ttgcagcgtg
    13681 cgctctttcg gcggcggcgt ggtcagcggg tgatccgctt tgaacgggcc ttccggcatg
    13741 ttgttgaggc actgctcaag aatgcgcaga ctctggcgca gctcttccac tttcagcatt
    13801 acgcgggtgt agcagtcaga aacaccacca ccaaccggga tttcaaagtc gaagttttca
    13861 tagccagaat aaggacgcgc cttacgcacg tcgaagtcga tcccggtagc acgcaggccc
    13921 gcgccagtgg tgccccactc cagcgcctct ttcgcgccat aggcggcaac gccctgggaa
    13981 cgacctttca gaatggtatt ttgcagcgcc gctttctcgt aagacgccag acgtttcggc
    14041 atccagtcga ggaactcacg cagcaggcga tcccagccgc gcggcaggtc gtgcgctacg
    14101 ccgccaatac ggaaccacgc cgggtgcata cggaaacccg tgatcgcttc caccagatcg
    14161 taaattttct gacgatcggt aaaggcgaag aacacgggcg tcattgcgcc gacgtcctga
    14221 ataaaggtcg agatgtacag caggtggctg ttgatacgga acagttcgga gagcataacg
    14281 cgaatgacgt taacgcgatc cggcacggtg atcccggcca gtttctctac cgccagcacg
    14341 taaggcattt cgttaacgca gccgccgagg tattcaatac ggtcggtata cggaatgtag
    14401 ctgtgccagg actggcgctc gcccattttc tcagcaccac ggtggtggta gccgatgtct
    14461 ggtacgcagt cgacaatctc ttcgccgtcg agttgtaaaa cgatacggaa agccccgtgc
    14521 gccgacgggt ggttcggacc gaggttgagg aacatgaagt cctcgttttc ggtgccgcgc
    14581 ttcatccccc actcttccgg tttgaaggtc agggcttcca tctccagatc ctgtttggct
    14641 ttggtcagct caaacggcga gaattcggta gcgcgcgccg ggtaatcttt acgcagcggg
    14701 tgacctttcc aggtttgcgg catcatgatg cggcgcaggt tcgggtgacc gtcgaaagta
    14761 atgccaaaca gatcccaggt ttcacgctca taccagttag cgttcgggaa cagtttggtg
    14821 aaggtcggta cgtgcaggtc gttttctgcc agcgccacct tcagcatgat gtcgcggtta
    14881 cgatcgatag aaatcagatg gtagaaaacg gaaaaatccg cggcaggtaa cccttcgcgg
    14941 tgtgtgcgca gacgttcgtc catgccgtgt aagtcaaaca gcatgacgta aggtttcggc
    15001 agtttcttta agaaatcgcc aacttccagt aattgttcac gcttgatcca cacaacggga
    15061 accccggtgc gagtcgcctg aacagtaaag gcatccggcc caaaacggtt gcgcagttcg
    15121 ccaattaccg gatcatcaag atgatcgcgg gtctgccagg cgggttcttg cgcggttaag
    15181 tcggtcatat tgttcaccat tgcaaatggt ccgtggtgac tgtcggcgca gtaagcttcg
    15241 cgaaatagat ttaggaataa gcgaagaaaa atccctttgc cgacaggcgc aaattaaatc
    15301 tcgtcaggtg tacgcaggtt ggttacggca atgcgttcac cgcgcttgcg ttcgcgctct
    15361 gattgcatat tggcgcgata aacgccctga tcgccaacca cccaggagag cggacgacgt
    15421 tctttgccga tagattcctg caacagcatc agtgcctgca tgtacgcttc aggacgcggc
    15481 gggcagcccg ggatatacac atcaaccggg atgaatttat cgacgccctg cacaacggaa
    15541 taaatatcgt acataccacc agagttggca caggcaccca ttgagataac ccattttggt
    15601 tccagcatct ggtcatacag acgctgaata accggtgcca ttttggtaaa gcaggttcct
    15661 gcaaccacca tcaggtcagc ctgacgcggc gaagcacgca atacttctgc gccaaaacgc
    15721 gccacgtcat gcaccgcggt aaacgaagtc accatctcaa cgtaacagca ggaaagaccg
    15781 aagttatacg gccaaattga gtttttacga ccccagttaa ccatgtcatt gagcttgccc
    15841 ataaacacgt ttttgttaac ttcttgctcc agagggtcgg ttacgatctc ctgcttttgc
    15901 agggggtaac ggtcgttctc accgttggga tctatgcggg tgagcgtata atccatctta
    15961 atgcctcgcg gttagcgttg acgattagcg atactgttcg tttccgggtt catacgctcg
    16021 cggcgtgaac gcgcgggcgt ccagtccagc gcgccaatac gcaccagata aaccagacct
    16081 gccagtaaca caaaaataaa aattgcagct tccacaaagc ctacccagcc gctttcgcgg
    16141 atagaggttg accatgcgaa cagatacagc gcttcaacgt cgaagataac gaagaacatg
    16201 gccaccagat aaaacttggc ggacaggcgt aagcgggcgg agccgaccga gtcgataccg
    16261 gattcaaacg gcacgttttt cgacctcgcg cgtgcgcgac cgcctaaaaa ccaaccacct
    16321 accagcatta ggcaacacag gccaatggca acgataagaa agatagcgaa tgcccagtga
    16381 tgagcgatga cttcagtgga tgttgacata ctcattgctt actcatcaaa agtagcgcca
    16441 gattcactgc tcttcacggc agatggacgc cacatcgatt catggggagg aataaaaaaa
    16501 accttacaat cactgtagaa attcttttat acagctaatt gatgtggtct tttactcctt
    16561 tctataacct tttgtcaact ttaacaaaag tttcttcaca ttagtttaca taatatcaac
    16621 accattagca tttaatgccc tttcacccca gatccttgac gactccagga taattagatg
    16681 ttgttgaatc gtgtccgttg tgaagcaatg gaaaaaatac gggtctattt tgacaggaat
    16741 ttgtgtcgat tcctcccccc aaaagagagt attttcttga tctgtgacac gcttttgtca
    16801 ttccataaca aaaacgcagc aacaaattta cgtatttttt aacatcattg tagcaggtga
    16861 tttttttcag gcgattattt gtgcgttcgg gacgtgaatc tctggtgatt aaaaaatgaa
    16921 cagttttgta cgttctgcac tatgcgagga aggattttta ctaaaaaaaa gccgctgggg
    16981 tttaaaacac ccccagcggc tcgtttttta cactattgtc tcaggaatta tctatcgtcc
    17041 gtcgattact cgatatccct ttcaatcaac aatgaatcat ccccttccgg agcagacatt
    17101 gggctgtatt gccacggatt atggtagctc tccatcgcct gatagatcac ctgggccagc
    17161 tcattattac tggacgggtc atagcacagc aaatattcgg tatcaggtag cggcggtaag
    17221 ccatctactc cactcagcac acgcaggtcc gggctcatca tctcaaccgg ccttgccgtc
    17281 acgccaagac cggctttcac tgccgcacga acggccggaa gcgtcgaggc gacataagcc
    17341 agtcgccatg gaatatctgc tttattaagc gttgccagca ccatatcgcg aaacggactt
    17401 ggatcatcca gcaatacaag ggggatcggc tcgccttttt gcaatacgta ttccgctgcg
    17461 cagtaccagt gtgttggcga ggtacgcagg ttcaatgcct taaacgctga aggacgatgg
    17521 gtggttacca tcaaatccac ttcctgggat tccagcattt ctgccatata ggcattacgt
    17581 ttaacgcgga catccagcgc taatttcgga taaaccgaac tcacgcgatt taacaggaaa
    17641 ggtaagatcg tatcggcaga ttcatctgaa gcaccgatag ttaacacgcc ctgaagatta
    17701 ctgaacatta atgatgagca ggcctcatca ttaaaacgca ggattttcct ggcgtaacca
    17761 agaagttgaa tgccatgttc agttaacagt ttgttgcgac cgtgacgagc gaacagttct
    17821 ttcccaacga gttgttccag acgctgcatt tgctgactta cggcggactg agtacgacac
    17881 acagcggcag ctgcggcagc aaaagtgttc agatcggcaa cagcaacaaa tgttctcagc
    17941 agatcgaggt cgaggttaat tatcggacga tttgcactta tcatatatta tcacttactg
    18001 gcggctcata ctgagctggt taatgctgtg cacacacaaa caagcaattc catttgtaat
    18061 gtgcctccct ggcagtttca tccggaaccc ggacgaaagt aaaaatgcat atgagttgca
    18121 ctaaaaaagc gactcacatt gttccgttat aatgcctgaa gtagatcaca gaatatatct
    18181 tcagggatcg catatctatt aagttactca ctcttttcta tttatgacat gcgcgtgttt
    18241 gtataaatgt aaatgtgagt ccttgttcca ctctcgtgca gcatcgctgg tcatacgcga
    18301 acacgtacaa acagcaatgg tgaggcggca tcaagagcag ggatccgtca tttatgcgag
    18361 cattttaccc caaaaacctt ttatttataa gggccattgc gaatcatctg atgcaaagtt
    18421 atgttatgtt aggcaaagta atcttctatt attaataagc acatcaaaac ctttttgaat
    18481 attaaataat aattaattag catcatcctc attcattaat tccgcttaac aatagtttca
    18541 caccttgccc cattacgaca cctcggaaat catcacgctg ttaaactctc cgtaatgcgg
    18601 aaatatcatc atcgcgtttc attagtgaat tcttaaatga ggcattttca cacaattatc
    18661 ttacagataa aaaaaccaga cttacaatta agaatcagaa caattcacta tataacattg
    18721 catgtaaagc atatacacct cattattttg tcatcattaa gttattaaca gcacaatcga
    18781 gccttcccct ctggcaaaat cttattctgc agaccttcaa aacaccgtcc tgggggagta
    18841 cattgttcta agctgacttc cacggcaggg agtggcgata acagcaaaaa aggtcaagat
    18901 tcatgtcccc cattgaaaaa tccagcaaat tagagaatgt ctgttatgac atccgtggtc
    18961 cggtgctgaa agaagcaaaa cgcctggaag aagaaggtaa caaggtactg aaactgaaca
    19021 tcggcaaccc agccccgttc ggttttgacg cgccagatga aatcctcgtt gacgtgatac
    19081 gcaacctgcc taccgctcaa gggtattgcg attccaaagg tctttactcc gcgcgtaaag
    19141 ccatcatgca gcactaccag gctcgtggca tgcgtgatgt taccgtggaa gatatttaca
    19201 tcggcaatgg tgtatcggag cttatcgttc aggcaatgca ggcattgctg aacagcgggg
    19261 acgaaatgtt ggttcctgca ccagattacc cactatggac cgcggcggtt tcgctttcca
    19321 gcggtaaagc ggtgcattat ctttgcgatg aatcctctga ctggttcccg gacctcgatg
    19381 atattcgcgc taaaattacg cctcgtacgc gtgggatcgt tattatcaac ccaaataacc
    19441 caaccggcgc ggtatattcc aaagagcttt taatggagat tgtggagatt gcacgtcagc
    19501 ataatctcat tatcttcgcc gatgaaattt atgacaaaat tctttacgac gacgctgagc
    19561 atcactcaat tgcgccgctg gcacctgacc tgctgaccat tacctttaac ggactgtcga
    19621 aaacgtaccg cgttgcaggc ttccgtcagg ggtggatggt gttgaacggg ccgaaaaaac
    19681 acgccaaagg ctacatcgaa ggtctggaaa tgctggcttc aatgcgcctg tgtgctaacg
    19741 ttcctgcgca acacgccatt cagaccgcgc tgggtggtta tcagagcatc agtgaattta
    19801 ttacccctgg cggtcgtctt tatgagcagc gtaaccgcgc gtgggaactg atcaacgata
    19861 ttccgggcgt ttcctgcgtg aaacctcgtg gtgcgctgta tatgttcccg aaaatcgacg
    19921 ccaaacgctt taacattcac gacgatcaga aaatggtgct ggatttcctg ttgcaggaaa
    19981 aagttctgtt ggtgcaaggg acggcattca actggccgtg gccggatcac ttccgcattg
    20041 tcacgctacc gcgtgtcgat gatatcgagc tgtctttgag caagttcgcg cgtttccttt
    20101 ctggttatca tcagctgtaa tcttaatttc actgccggag attgcatccg gcagcgttat
    20161 cccgccacaa tgacctgatg atgtcatcat acgtaaggtc actatgaaac agagccattt
    20221 ttttgcccat ctctcccgcc tgaaactcat taaccgctgg ccgctaatgc gcaacgtgct
    20281 gacggaaaat gtgtccgaac acagtttgca ggtagcgatg gtcgcccatg cgctggcagc
    20341 tatcaaaaat cgaaaatttg gcggtaatgt caacgccgaa cgtatcgctt tactggcgat
    20401 gtaccacgat gcctcagaag tgctcaccgg cgatctccct actccggtga aatacttcaa
    20461 ttcgcaaatc gctcaggagt acaaggctat tgaaaaaatc gctcagcaaa aactggtcga
    20521 tatggttccg gaagagctgc gggatatctt tgcgccgtta attgacgagc atgcatatag
    20581 cgatgaagaa aaatcgctgg tgaaacaggc agatgcactg tgtgcatatc tgaaatgtct
    20641 ggaagaactc gcggccggaa ataatgaatt cttgctggca aaaacgcgac tggaagcgac
    20701 gcttgaagcg cgtcgcagcc aggagatgga ctacttcatg gaagtatttg ttcccagctt
    20761 ccatctttcg ctcgatgaga ttagccagga ttcaccgctg taagcagccg gagtctgcgt
    20821 cgcatcaggc aataagcgcc ggatgcgaca tcaggctctt gtcaaaacgg aaacagcatc
    20881 gggatcatca ccacacaaac cgccatcacg ataatggtga acggtacccc caacttcaca
    20941 aagtcactga agctgtaatt tcccggacct aaaaccagtg tgttaacagg tgaagaaacc
    21001 ggtgtcataa aggcggcgga tgctgccatc gccacgacca tcgcgaatgg ataaggcgac
    21061 acccccatcg ttttggcagc agccagcgca atcggagcca tcaacaccgc cgtcgcggta
    21121 ttagagataa atagcccaat aaccgccgac aagacaaaca aacaccccag catcatatgt
    21181 ggcccgtaac caccgccaat gtccatcagc cctttcaccg ccagcgcgac acctcccgtt
    21241 ttctgtaatg ccacagcaaa cggcatcatc ccaacgatca aaataatgct cggccagtga
    21301 atggatttat aggcgctttc agcatctata cagcggaatt tccccatcag caggcaggcg
    21361 atgatagcgg caacaggatt aggaatttca tctgtcagca ttaacgccac cattagcacc
    21421 agacagaaaa tggcatgggg tgcctggctg tgcgcgggcg atgcttcact cacctcttcc
    21481 ggtaagttca gcgctacgaa gtcgcggccc tgtttggcca gcataccgat cagtttccag
    21541 ttacccacaa ccaggatgat atcgcccagc agcagaggct catccgccag cgaaccttcc
    21601 agcgccacgc cattgcgctt tagccccacc acattcagtc cgtagcgggt acgaaaacca
    21661 atttcgcgca ccgatttacc aatcagttct gactcaggaa ttaatgaaat ctctgccatg
    21721 cccacatcaa gggcctggtc agaaaaatac tcgccgcgca gtaccatcgg ctccagcaat
    21781 tgctcactac aaaattgccg gagatcgaca tcagccgcag acatatcaat aagcaaaacg
    21841 tcacgcgcgc gaaattcaga aaccccatta acgttcacga taacgcgacg aaaacgccgc
    21901 cagcgttcaa caccgatgac gttagcgcca taacgctcac gtaatttgag atcatccagc
    21961 cgttgaccaa tcattggcga tccggggcga atagccagac gtcgcgcacg cccggtcagt
    22021 cgatattcac ggataagatc gcgaaaggtt cgacgcgtcc agccttcgcg ctgcggggtc
    22081 tgggtatccc ctttcagcat gaaacgcatc actaacatat acaaaatacc cagcaccagc
    22141 acgaccaggc caacaggtgt tacgctaaag aaactgaagc cgtgatagcc ttcacgcagc
    22201 aattcactgt tgactaccag gttcggcggc gtcgccacca gcgtcatcat gccgctaatc
    22261 agcccggcaa aacttaacgg catcatcaga cgtgacggcg aagtttgcat acgcatggca
    22321 acgcttaaca ccacgggaat aaagatagcg acaacgccgg ttgaactcat aaacgcgcca
    22381 agccccgcga cggtcagcat caacaaaacc aacattttga tttcactgtt gcccgccact
    22441 ttgaccagcc atgttcccat tacggtggca acaccggtac ggaccaaacc atcgccaata
    22501 ataaacaagg cggcaatcag gacaacgtta ggatcagaaa agccggaaaa tacttcgggg
    22561 accgtcagcg ttccgcttaa tgcaaacgcg acaataacaa acaaagcgac cgcatccata
    22621 cgcactctgc ccgtcgcaaa caagacgatg gcaacggcca gtaatgaaag aacccaaatc
    22681 aattcaccgt tcacaactta tccttgttaa ttgaggggga tgacttgatt ctgccataaa
    22741 aaagccccga cgagacgggg ctaaatcatg atcaggtgtt tcactgaata ataacatcgc
    22801 catttagctg tttggtcaca gtaatttgct ccagactgtg gaggaccaaa tcgacctcat
    22861 tcaggcgcgg ggtatctgcc ggagcgttaa ccgcaatgac atggcaaccc gccgccaggc
    22921 cagaaagcac gccagcggga gcatcttccg ccaccacaca ctcctgcggc gcaagcccca
    22981 gcagctgcgc acctaacaga tacgcatcag gttctggttt tccgcgcttc actcgctcag
    23041 cggttacaaa cacctttggt gcgggaagcc cagctatttt atggcgcgct cgcgctaccg
    23101 gcatggagcc agaagtcaca atggcccacg gaatacctgc tttattcaaa tgattgagta
    23161 aggcgattgc ccccggaagc gcggtaatac cttcggtttc cgtggcctcg atgtgctcca
    23221 gacacgtaaa ctcggcggca atatcagcct cggatttgcc cgccataaaa tggcgcaaag
    23281 aggtgatcgc ctgtttaccg tgaatgaaag ccagcacctc ttccggcgct aacccatgac
    23341 gtctggccca gttgctccac gcccgttcta ccgcaggcag ggaatccacc agcgttccat
    23401 caagatcaaa cagaaaacct ttgcaccgca cgcgggcctc ctcaggcgtt gataatttga
    23461 ttaatttcgt tggcgctcaa atggtactga cgcaggcagg catgccacac attaagcatg
    23521 cgctgatatt tttcccacat tggcgtctgg gcgttaaaac catgagtccc agcgtcaaaa
    23581 tgggtatagc gcccttccac attaaccata aagcggacat aaccgaggta acgtgcttca
    23641 gtggcggcgt caaagccgag gaaggtgaca cgacgttcat cgatggattg ctggtcctgc
    23701 aaattagacc aggaaacatg caacgcatga tacatctcca taatgtcgat gatagtgcgg
    23761 caggtttctt ctttcagctc gccaaactcg cgatccaatt cacgcatctg taatccgtaa
    23821 ccacgctcaa taattgtttg caggcgacgg taacgttcag catttgccgg atcgagcata
    23881 gtcatcatct tgtactgatt agacaaaata agacgttgcg cgttggtcat ttccattgtt
    23941 gactcctgta tcactctact acggtgaaaa aaaaagaagg ctgagtatgc cttcttttat
    24001 atgcgtaatc aggggtcaat tacaaatcat caaggaaagt tttatccagt tgtttgaagg
    24061 cgcgcttaag cgtgtcagct aatgcctggt aatcaggctt gccttcaacg ggtgccaaca
    24121 cctgtccaga ctcctgcaat ttaccgcgaa cttcataaaa ccagttgagg atagcagggg
    24181 gtaatggcgt tacagaacgc ttgcccagcc accataatcc ctgcatgggt aaacttaagg
    24241 cgaacagcgc agtggcaact gccggcccaa gctgaccgcc cagggcaatc tgccagcaga
    24301 gagtaaatac ggcgatcggc ggcataaaac ggatcgcata acgcgtcatc ttgataacgc
    24361 gattttcgac aaagaccggg gcaaggcgtt tttccagcgg ccacgtcttt gagtaatgct
    24421 gtccccggcg aaacaagcta aaaaaattaa cagaacgatt atccggcgtt gacatgcttc
    24481 acctcaactt cacatataaa gattcaaaaa tttgtgcaaa ttcacaactc agcgggacaa
    24541 cattcaaaac attttgtctt ccatacccac tatcaggtat cctttagcag cctgaaggcc
    24601 taagtagtac atattcattg agtcgtcaaa ttcatataca ttatgccatt ggctgaaaat
    24661 tacgcaaaat ggcatagact caagatattt cttccatcat gcaaaaaaaa tttgcagtgc
    24721 atgatgttaa tcataaatgt cggtgtcatc atgcgctacg ctctatggct ccctgacgtt
    24781 tttttagcca cgtatcaatt ataggtactt ccatgtcgag taagttagta ctggttctga
    24841 actgcggtag ttcttcactg aaatttgcca tcatcgatgc agtaaatggt gaagagtacc
    24901 tttctggttt agccgaatgt ttccacctgc ccgaagcacg tatcaaatgg aaaatggacg
    24961 gcaataaaca ggaagcggct ttaggtgcag gcgccgctca cagcgaagcg ctcaacttta
    25021 tcgttaatac tattctggca caaaaaccag aactgtctgc gcagctgact gctatcggtc
    25081 accgtatcgt acacggcggc gaaaagtata ccagctccgt agtgatcgat gagtctgtta
    25141 ttcagggtat caaagatgca gcttcttttg caccgctaca caacccggct cacctgatcg
    25201 gtatcgaaga agctctgaaa tctttcccac agctgaaaga caaaaacgtt gctgtatttg
    25261 acaccgcgtt ccaccagact atgccggaag agtcttacct ctacgccctg ccttacaacc
    25321 tgtacaaaga gcacggcatc cgtcgttacg gcgcgcacgg caccagccac ttctatgtaa
    25381 cccaggaagc ggcaaaaatg ctgaacaaac cggtagaaga actgaacatc atcacctgcc
    25441 acctgggcaa cggtggttcc gtttctgcta tccgcaacgg taaatgcgtt gacacctcta
    25501 tgggcctgac cccgctggaa ggtctggtca tgggtacccg ttctggtgat atcgatccgg
    25561 cgatcatctt ccacctgcac gacaccctgg gcatgagcgt tgacgcaatc aacaaactgc
    25621 tgaccaaaga gtctggcctg ctgggtctga ccgaagtgac cagcgactgc cgctatgttg
    25681 aagacaacta cgcgacgaaa gaagacgcga agcgcgcaat ggacgtttac tgccaccgcc
    25741 tggcgaaata catcggtgcc tacactgcgc tgatggatgg tcgtctggac gctgttgtat
    25801 tcactggtgg tatcggtgaa aatgccgcga tggttcgtga actgtctctg ggcaaactgg
    25861 gcgtgctggg ctttgaagtt gatcatgaac gcaacctggc tgcacgtttc ggcaaatctg
    25921 gtttcatcaa caaagaaggt acccgtcctg cggtggttat cccaaccaac gaagaactgg
    25981 ttatcgcgca agacgcgagc cgcctgactg cctgatttca caccgccagc tcagctggcg
    26041 gtgctgtttt gtaacccgcc aaatcggcgg taacgaaaga ggataaaccg tgtcccgtat
    26101 tattatgctg atccctaccg gaaccagcgt cggtctgacc agcgtcagcc ttggcgtgat
    26161 ccgtgcaatg gaacgcaaag gcgttcgtct gagcgttttc aaacctatcg ctcagccgcg
    26221 taccggtggc gatgcgcccg atcagactac gactatcgtg cgtgcgaact cttccaccac
    26281 gacggccgct gaaccgctga aaatgagcta cgttgaaggt ctgctttcca gcaatcagaa
    26341 agatgtgctg atggaagaga tcgtcgcaaa ctaccacgct aacaccaaag acgctgaagt
    26401 cgttctggtt gaaggtctgg tcccgacacg taagcaccag tttgcccagt ctctgaacta
    26461 cgaaatcgct aaaacgctga atgcggaaat cgtcttcgtt atgtctcagg gcactgacac
    26521 cccggaacag ctgaaagagc gtatcgaact gacccgcaac agcttcggcg gtgccaaaaa
    26581 caccaacatc accggcgtta tcgttaacaa actgaacgca ccggttgatg aacagggtcg
    26641 tactcgcccg gatctgtccg agattttcga cgactcttcc aaagctaaag taaacaatgt
    26701 tgatccggcg aagctgcaag aatccagccc gctgccggtt ctcggcgctg tgccgtggag
    26761 ctttgacctg atcgcgactc gtgcgatcga tatggctcgc cacctgaatg cgaccatcat
    26821 caacgaaggc gacatcaata ctcgccgcgt taaatccgtc actttctgcg cacgcagcat
    26881 tccgcacatg ctggagcact tccgtgccgg ttctctgctg gtgacttccg cagaccgtcc
    26941 tgacgtgctg gtcgccgctt gcctggcagc catgaacggc gtagaaatcg gtgccctgct
    27001 gctgactggc ggctacgaaa tggacgcgcg catttctaaa ctgtgcgaac gtgctttcgc
    27061 taccggcctg ccggtattta tggtgaacac caacacctgg cagacctctc tgagcctgca
    27121 gagcttcaac caggaagttc cggttgacga tcacgaacgt atcgagaaag ttcaggaata
    27181 cgttgctaac tacatcaacg ctgactggat cgaatctctg actgccactt ctgagcgcag
    27241 ccgtcgtctg tctccgcctg cgttccgtta tcagctgact gaacttgcgc gcaaagcggg
    27301 caaacgtatc gtactgccgg aaggtgacga accgcgtacc gttaaagcag ccgctatctg
    27361 tgctgaacgt ggtatcgcaa cttgcgtact gctgggtaat ccggcagaga tcaaccgtgt
    27421 tgcagcgtct cagggtgtag aactgggtgc agggattgaa atcgttgatc cagaagtggt
    27481 tcgcgaaagc tatgttggtc gtctggtcga actgcgtaag aacaaaggca tgaccgaaac
    27541 cgttgcccgc gaacagctgg aagacaacgt ggtgctcggt acgctgatgc tggaacagga
    27601 tgaagttgat ggtctggttt ccggtgctgt tcacactacc gcaaacacca tccgtccgcc
    27661 gctgcagctg atcaaaactg caccgggcag ctccctggta tcttccgtgt tcttcatgct
    27721 gctgccggaa caggtttacg tttacggtga ctgtgcgatc aacccggatc cgaccgctga
    27781 acagctggca gaaatcgcga ttcagtccgc tgattccgct gcggccttcg gtatcgaacc
    27841 gcgcgttgct atgctctcct actccaccgg tacttctggt gcaggtagcg acgtagaaaa
    27901 ggttcgcgaa gcaactcgtc tggcgcagga aaaacgtcct gacctgatga tcgacggtcc
    27961 gctgcagtac gacgctgcgg taatggctga cgttgcgaaa tccaaagcgc cgaactctcc
    28021 ggttgcaggt cgcgctaccg tgtttatctt cccggatctg aacaccggta acaccaccta
    28081 caaagcggta cagcgttctg ccgacctgat ctccatcggg ccgatgctgc agggtatgcg
    28141 caagccggtt aacgacctgt cccgtggcgc actggttgac gatatcgtct acaccatcgc
    28201 gctgactgcg attcagtctg cacagcagca gtaatctcgt catcatccgc agctttgcgc
    28261 tgcggatatc tgaaccggaa ataatcacta tttccggttt tttattctct taatctgcat
    28321 taatcctttc tgattatctt gcttaactgc gctgcatcaa tgaattgcgc catttcactt
    28381 tgcatactta ccactttgtt ttgtgcaagg gaatatttgc gctatgtccg caatcactga
    28441 atccaaacca acaagaagat gggcaatgcc cgatacgttg gtgattatct tttttgttgc
    28501 tattttaacc agccttgcca cctgggtagt tccggtgggg atgtttgaca gtcaggaagt
    28561 gcagtatcag gttgatggtc aaacaaaaac acgcaaagtc gtagatccac actcatttcg
    28621 cattctgact aacgaagcag gcgaacctga gtatcaccgc gtacagctgt tcacgacggg
    28681 cgatgaacgc ccgggcctga tgaacttccc gtttgaagga ttaacctcag gatcgaaata
    28741 cgggacagct gttggcatca tcatgtttat gctggtgatt ggcggcgcgt ttggcattgt
    28801 gatgcgtaca ggaaccattg ataacggtat cctggcgctt attcgccata cccgcgggaa
    28861 tgaaattctc tttattcctg cgctgtttat tctgttttca cttggcggcg cggtatttgg
    28921 tatgggagaa gaggccgtcg cctttgccat tatcatcgca ccgctaatgg tccggctggg
    28981 ctatgacagt attaccaccg tcctggtgac ctatattgcc acgcaaatcg gttttgccag
    29041 ttcgtggatg aacccgtttt gtgtggtcgt tgctcagggg attgccggcg ttccggtgct
    29101 ttctggctcc gggttgcgca tcgtggtgtg ggttatcgcc actatgattg gcctgatctt
    29161 taccatggtg tacgcctcac gagtgaaaaa gaatcctctt ctgtcacgcg tgcatgagtc
    29221 cgaccgcttc tttcgtgaaa agcaggcgga tgttgaacaa cgtccgttta cctttggtga
    29281 ctggctggta ttgattgtcc tgaccgccgt aatggtctgg gtgatttggg gcgtgatcgt
    29341 taatgcctgg tttattccag aaattgccag ccagttcttc accatgggtc tggtgattgg
    29401 catcatcggc gttgtttccc gccttaacgg catgacggtt aataccatgg cttcatcctt
    29461 taccgaaggg gcgcgaatga tgatcgcccc tgccctgctg gtgggtttcg ccaaagggat
    29521 tttgctgctg gtcggtaatg gtgaagcggg tgatgccagc gtgttaaata ccatcctcaa
    29581 cagcattgcc aatgccatta gcggtctgga caacgcggtc gcggcctggt ttatgttgct
    29641 cttccaggcg gtatttaatt tcttcgtgac gtccggttct ggtcaggcgg cgttaaccat
    29701 gccgttactg gcaccgcttg gcgatctggt cggtgttaac cgtcaggtta ccgtgctggc
    29761 attccagttt ggtgatggct tcagtcacat catttaccca acctcagctt cgttaatggc
    29821 aactctcggt gtgtgcaggg tggacttccg taactggctg aaggtgggcg cgaccctact
    29881 tggactgctg tttattatgt ccagcgtcgt agtgatcggc gctcagttga tgggctacca
    29941 ctaaaaatgt taagagccgc aattgcggct cttttttcat tctgccgttt cagtctctac
    30001 ggcttcattt ttggcattgc gcttcatcca caacgccagc gctttcagcg agtctggagt
    30061 gaactcatcg cagcgtgcgg tgatttcttc cggcgtcagc cagcaaactt cactgacttc
    30121 atcttcctgt agggcgaagg gaccgtgaga gacgcagctg aacaacgcgc cccagacacg
    30181 gcaattttta tcttcgaaat agaactgccc gtgctcggca aaggggacac cggcaatgcc
    30241 caactcttct tccgcttcgc gacgcgcgga ttccagcagt tgctcatcgg cctgaaccac
    30301 accgcctgcg gtcgcatcta acatgccggg taaaaagtct tttgtctcgg tacgacgctg
    30361 gaccagaatt ttgcccatgc catcatgcac gacgatgtaa gttgcacgat gacgcagaca
    30421 ctgtgcccgc atttgttccc ggctggcttg tgcaatgact tcgttctctt cattgacaat
    30481 atccacccat tcagtacttg ccaaacgacg ctgttccatt atcaggaaac cttctttttc
    30541 tggcgctctt acggcgcatt ttgagttgtg ggtaaattac ggattaatcg cgacctgcgc
    30601 aatgatactt tgatcattga gtgcgattac actaagtacg tcattatcca gcatgccata
    30661 actcgccgga ttaccgcctt tcggaatact caccgagccg gggttgaagt ggaaaatctc
    30721 cccccgctgt tctgccactg gcagatgggt atgaccgtac accagcacat cgttctggtt
    30781 taaagcaggt agattttccg ggccaaaaag atgaccatgc gtcaaaaaca gacgttgttt
    30841 ttccagtaat acctgttgcc acggcgcggt tatcgggaaa tgcagcagca tttgatccac
    30901 ttcgctgtcg cagttgccgc gcacagcgat aaccttatgt gccacttcat taagccgttc
    30961 agcgactttg gctggcgcgt aaccctccgg taaagcatta cgcgggccgt gattcaacac
    31021 gtcgccaagg atcaccagcc actgggcacc gctttgggca aacaactcca gaacacgttc
    31081 cgtcgccggt aacgacccat gaatgtccga tgcaaacatc agtttcatca ctcactcctc
    31141 gtcgaaaaaa cgtaagctat gatactggat tctgtcgttg ctatcagcca gattgcttcg
    31201 ccgaaagtgc aataaaacgc tggacagacg ctcgctgcca ctggaatgtc gcataatcca
    31261 ccaggcgttc tggcacctca tcgccatgta gtaccaggcg gttaatcatt agcgccagat
    31321 cagtatcagc aatgcaccat tcaccaaata aattcggctg acccagtgct aacaaatgtt
    31381 ctgccatcgc gacactggct tttccctcgg ccgttagtgg cgcttttttc gcccccgcaa
    31441 agacaacatc cgtcggacgc tcttcgcgga tgggcatcag atcgctgcgc agccaggcct
    31501 gaatctgtcg tgcacgcgca cgattttcta aatcaagcgg ataaatacgt tcccaggtcg
    31561 gtggcgcaaa tcgatcttcc agatactccg caatggcaga agattcactc aactcaaaat
    31621 catcgatttg taataacggc acacggcgtg tctgaccgta accttgccac gtcggctgca
    31681 aatgttcacc gctgtcgagg tcgatggtct tgatatgaaa cgacaggcct ttttcctgca
    31741 acgccaccca agcggataac acataagggg agaaaaagtg ggcatctgac caaagcgtga
    31801 tagcgggttt actcattatg tcctcggcaa taaaggaggc tggctatcaa actatagagc
    31861 ctttttgtca ctgtcacgtg acgaatactc acgtaagtcg ccgcgctttc tatacttgtg
    31921 gtgttcagat gattaaacaa ccggagctgc aatgatcgat ctctatttcg ccccgacacc
    31981 caatggtcac aaaattacgc tgtttctcga agaagcagag cttgattatc gcttgattaa
    32041 ggtagacctg gggaaaggcg gtcagtttcg tccggaattt ttgcgcattt cgcccaacaa
    32101 caaaattccg gcaattgttg atcattctcc tgccgatggc ggcgaaccgc taagcctctt
    32161 tgaatctggt gccattttgt tgtatctggc tgagaaaacg gggctctttt tgagtcatga
    32221 aacgcgtgaa cgcgccgcca cattacagtg gttattctgg caggtaggcg aactggggcc
    32281 gatgcttggg caaaatcatc attttaatca cgcagccccc caaaccattc cttacgctat
    32341 tgaacgttat caggttgaaa ctcagcgtct ttaccatgta ctaaacaagc ggctggaaaa
    32401 ctcgccctgg ctgggaggcg agaactacag cattgcggat attgcctgct ggccgtgggt
    32461 taatgcctgg actcgccagc gaattgacct cgcaatgtat ccggcagtca agaactggca
    32521 tgagcggatc cgttcgcgcc ctgccaccgg gcaggcactg ctaaaggcac aactcggtga
    32581 tgagcgttcg gatagttaac agaaacaggt tctcgtgtat tatttcatcc taagtaaaac
    32641 aacggagaac ctgcaatggc acaacctgcc gctattattc gtataaagaa ccttcgttta
    32701 cgtacgttta tcggaattaa ggaagaagaa attaacaacc gtcaggatat tgttatcaat
    32761 gtgacgatcc actaccccgc cgataaagcg cgcactagcg aagatatcaa cgatgcgctg
    32821 aattatcgca ccgtaacgaa aaacattatt cagcatgtag agaataaccg tttctctttg
    32881 ttggaaaaat taactcagga tgtgctcgat atcgcacgtg aacatcactg ggtgacgtat
    32941 gctgaagtgg agatcgataa actgcacgcg ctgcgctacg ccgattcggt atccatgacc
    33001 ttaagctggc agcgttaacc gccatatcgg gaggctgcat gaacatagtg atcaccggag
    33061 ggacgggatt aattggtcgc catttgattc cgcgtttgct ggagctgggc catcaaatca
    33121 cggtagtgac gcgtaaccca cagaaagcca gttccgttct cggccctcgg gtgacgctat
    33181 ggcaagggct agccgatcaa agcaacctca acggcgttga tgcggtaatc aacctggccg
    33241 gagaaccgat tgctgataaa cgctggactc acgagcaaaa agagcgtctc tgccaaagcc
    33301 gctggaatat cacgcaaaaa ctggtcgatt tgattaatgc cagcgacacg ccgccgtcgg
    33361 tactcatttc cggctcggca acgggctatt atggcgactt aggtgaagtg gtggttaccg
    33421 aagaggaacc gccgcataac gaatttactc ataaactctg cgcccgctgg gaagagattg
    33481 cctgccgggc gcaaagtgac aaaacgcgag tgtgcctgct gcgaaccggc gtggtactgg
    33541 caccggacgg cggtattctc ggtaaaatgc tgccaccgtt tcgtcttggc ctgggcgggc
    33601 cgattgactc tggtcggcag tatctggcct ggattcatat tgatgatatg gtcaacggta
    33661 ttctctggct gctggataac gagctgcgcg ggccatttaa tatggtttcg ccctacccgg
    33721 tacgcaatga acaatttgcc catgcgctcg gtcacgcgct gcatcgcccg gccattttgc
    33781 gcgtccccgc gaccgccatt cgactgttaa tgggcgaatc ttcagtactg gtattaggtg
    33841 gacaacgcgc gctgcctaaa cggctggaag aagcgggttt tgcgtttcgc tggtacgatt
    33901 tagaagaggc gctggcggat gtcgttcgct gatgtggttt acagcaaaca tccgccagtt
    33961 aactcccggt gttacaggat tagtggcttt gcgcgataag atcgtctggt gaaagtcggg
    34021 tcaccatcat aactaactct ctgtctaaac ctctatccag catctcctga gcaatacgca
    34081 gggcttcttc gtgtttgccc tgcattgcgc cttcttcacg taatctgtca gcaatggtca
    34141 tcagtttctc cttttcttgt ggtgcgcgtt ccgctatctc accaataaat gcacgaaaac
    34201 gctgggcatc ccctgtttgt aatacgtaat taaacagggc ttttagctgt ctgtcattag
    34261 tgttccctgt aactagcagc gaaacaattt ggtcgactaa tcccaacaga tcgcgctgac
    34321 gaatatgttt ctgaattaac tccaacagcg ccattttgcg gtgttgcata atctcgtcat
    34381 ccggcaccac ggtaatatcc accaacggaa acgctgatga atatattttg cgggctatag
    34441 caggctcggc aaattcatca agccagcaga gtgaataagg ataaggactt ctgcaaccat
    34501 gataaaacag catcgggatc accaatggaa gctctttata gcccgc
//
LOCUS       DAJTNN010000024        34140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTNN010000024 DAJTNN010000000
VERSION     DAJTNN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.024"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..142)
                     /locus_tag="OO150_001024"
     CDS             complement(<1..142)
                     /locus_tag="OO150_001024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515247.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGH"
     gene            complement(302..514)
                     /gene="rpmE"
                     /locus_tag="OO150_001025"
     CDS             complement(302..514)
                     /gene="rpmE"
                     /locus_tag="OO150_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5515248.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            717..2915
                     /gene="priA"
                     /locus_tag="OO150_001026"
     CDS             717..2915
                     /gene="priA"
                     /locus_tag="OO150_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5515249.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3071..4096
                     /gene="cytR"
                     /locus_tag="OO150_001027"
     CDS             3071..4096
                     /gene="cytR"
                     /locus_tag="OO150_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5515250.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4188..5147
                     /gene="ftsN"
                     /locus_tag="OO150_001028"
     CDS             4188..5147
                     /gene="ftsN"
                     /locus_tag="OO150_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5515251.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5240..5770
                     /gene="hslV"
                     /locus_tag="OO150_001029"
     CDS             5240..5770
                     /gene="hslV"
                     /locus_tag="OO150_001029"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5515252.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5780..7111
                     /gene="hslU"
                     /locus_tag="OO150_001030"
     CDS             5780..7111
                     /gene="hslU"
                     /locus_tag="OO150_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5515253.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7178..8104
                     /gene="menA"
                     /locus_tag="OO150_001031"
     CDS             7178..8104
                     /gene="menA"
                     /locus_tag="OO150_001031"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5515254.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDRPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8197..8682
                     /gene="rraA"
                     /locus_tag="OO150_001032"
     CDS             8197..8682
                     /gene="rraA"
                     /locus_tag="OO150_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5515255.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8767..9012)
                     /gene="zapB"
                     /locus_tag="OO150_001033"
     CDS             complement(8767..9012)
                     /gene="zapB"
                     /locus_tag="OO150_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5515256.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9438..10283
                     /gene="glpF"
                     /locus_tag="OO150_001034"
     CDS             9438..10283
                     /gene="glpF"
                     /locus_tag="OO150_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR5515257.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVKEKETTTPSE
                     QKASL"
     gene            10306..11814
                     /gene="glpK"
                     /locus_tag="OO150_001035"
     CDS             10306..11814
                     /gene="glpK"
                     /locus_tag="OO150_001035"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5515258.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRSE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11949..12959
                     /gene="glpX"
                     /locus_tag="OO150_001036"
     CDS             11949..12959
                     /gene="glpX"
                     /locus_tag="OO150_001036"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5515259.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13056..13802
                     /gene="fpr"
                     /locus_tag="OO150_001037"
     CDS             13056..13802
                     /gene="fpr"
                     /locus_tag="OO150_001037"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5515260.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIG
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13807..14235)
                     /gene="uspD"
                     /locus_tag="OO150_001038"
     CDS             complement(13807..14235)
                     /gene="uspD"
                     /locus_tag="OO150_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5515261.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14262..14561)
                     /locus_tag="OO150_001039"
     CDS             complement(14262..14561)
                     /locus_tag="OO150_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5515262.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSVDEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14773..15213)
                     /locus_tag="OO150_001040"
     CDS             complement(14773..15213)
                     /locus_tag="OO150_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5515263.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     CFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15314..15913
                     /locus_tag="OO150_001041"
     CDS             15314..15913
                     /locus_tag="OO150_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5515264.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16021..16788
                     /gene="tpiA"
                     /locus_tag="OO150_001042"
     CDS             16021..16788
                     /gene="tpiA"
                     /locus_tag="OO150_001042"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5515265.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEHGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16843..17598)
                     /gene="cdh"
                     /locus_tag="OO150_001043"
     CDS             complement(16843..17598)
                     /gene="cdh"
                     /locus_tag="OO150_001043"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5515266.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17705..18694)
                     /gene="sbp"
                     /locus_tag="OO150_001044"
     CDS             complement(17705..18694)
                     /gene="sbp"
                     /locus_tag="OO150_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5515267.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19014..19976)
                     /gene="pfkA"
                     /locus_tag="OO150_001045"
     CDS             complement(19014..19976)
                     /gene="pfkA"
                     /locus_tag="OO150_001045"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5515268.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20157..21059)
                     /gene="fieF"
                     /locus_tag="OO150_001046"
     CDS             complement(20157..21059)
                     /gene="fieF"
                     /locus_tag="OO150_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5515269.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21207..21719)
                     /gene="cpxP"
                     /locus_tag="OO150_001047"
     CDS             complement(21207..21719)
                     /gene="cpxP"
                     /locus_tag="OO150_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5515270.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQRS
                     TQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQ
                     AEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLK
                     LLSSSNSRSQ"
     gene            21869..22567
                     /gene="cpxR"
                     /locus_tag="OO150_001048"
     CDS             21869..22567
                     /gene="cpxR"
                     /locus_tag="OO150_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5515271.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22564..23937
                     /gene="cpxA"
                     /locus_tag="OO150_001049"
     CDS             22564..23937
                     /gene="cpxA"
                     /locus_tag="OO150_001049"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5515272.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24043..24717)
                     /gene="yiiM"
                     /locus_tag="OO150_001050"
     CDS             complement(24043..24717)
                     /gene="yiiM"
                     /locus_tag="OO150_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5515273.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24866..25831)
                     /gene="kdgT"
                     /locus_tag="OO150_001051"
     CDS             complement(24866..25831)
                     /gene="kdgT"
                     /locus_tag="OO150_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5515274.1"
                     /translation="MRKSRGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMITGTVSI
                     LAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVEVGFFAG
                     LSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGTAGIASF
                     EPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGL
                     LGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPMLIAEMVPAFKP
                     MAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26110..26730)
                     /gene="sodA"
                     /locus_tag="OO150_001052"
     CDS             complement(26110..26730)
                     /gene="sodA"
                     /locus_tag="OO150_001052"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5515275.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27014..28048
                     /gene="rhaT"
                     /locus_tag="OO150_001053"
     CDS             27014..28048
                     /gene="rhaT"
                     /locus_tag="OO150_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR5515276.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28045..28911)
                     /gene="rhaR"
                     /locus_tag="OO150_001054"
     CDS             complement(28045..28911)
                     /gene="rhaR"
                     /locus_tag="OO150_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5515277.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(28967..29803)
                     /gene="rhaS"
                     /locus_tag="OO150_001055"
     CDS             complement(28967..29803)
                     /gene="rhaS"
                     /locus_tag="OO150_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5515278.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30091..31503
                     /gene="rhaB"
                     /locus_tag="OO150_001056"
     CDS             30091..31503
                     /gene="rhaB"
                     /locus_tag="OO150_001056"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5515279.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            31500..32759
                     /gene="rhaA"
                     /locus_tag="OO150_001057"
     CDS             31500..32759
                     /gene="rhaA"
                     /locus_tag="OO150_001057"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5515280.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWGEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            33026..33850
                     /gene="rhaD"
                     /locus_tag="OO150_001058"
     CDS             33026..33850
                     /gene="rhaD"
                     /locus_tag="OO150_001058"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR5515281.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33860..>34140
                     /gene="rhaM"
                     /locus_tag="OO150_001059"
     CDS             33860..>34140
                     /gene="rhaM"
                     /locus_tag="OO150_001059"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5515282.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASADVCQRWWKYMTDVMPANPDNSPV"
BASE COUNT         8365 a         8909 c         8861 g         8005 t
ORIGIN      
        1 gatggccgga cgtcaccccg ccggggcaca ctgaacaggc cacgccggtg ggagcaccaa
       61 tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg tcgccctgac
      121 gcgccgccgg ttttccgctc atcgtaaaat ccttttgaaa taaataagaa ccagaagcct
      181 agcgccagtc aacaacagga aatgataatg agttaacacc ccggaatttt tcagaattta
      241 taaaaggcaa aattcaggca caaaaaaagc gccgtgcggc gcttttttcg gaaatccggt
      301 cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg ccaccggtag
      361 caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg tcgaggttca
      421 ggtcatgacc aacagtggag cggattttca ttacgttacc gcaagagcag ctagcagtaa
      481 tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt aaggccgcgt
      541 cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct tcaatacgat
      601 catttcgtac gaaggcgcga aatcatacag aaattaacca acgtatgcaa actgatccgc
      661 actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat gatgctatgc
      721 ccgttgccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat ctgctgccag
      781 aaggcatgac ggttaaagcc gggtgtcgcg tgcgcgtgcc gtttggcaaa cagcaggagc
      841 gcatcggggt tgtggtgtca gttagcgatg tcagtgagct gccgcttaac gagctgaaag
      901 cggtagtgga agtgctggac gttgagccgg tatttactca ctccgtctgg cggttactgc
      961 tctgggcggc agattactat catcatccga ttggcgatgt gctgtttcat gccttgccga
     1021 ttttactgcg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg tttgccactg
     1081 aacaaggcca ggcggtggat ctgaacagcc tgaaacgctc ccccaagcaa caacaggcgc
     1141 tggcggcgtt acggcaaggc aaaatctggc gcgaccaggt cgccacgctc gaatttaatg
     1201 atgccgcgtt gcaggcgcta cgcaaaaaag gtctgtgtga tttagcaagt gaaacaccag
     1261 agtttagcga ctggcgaacg aactatgccg tttctggtga gcggttgcga ttgaataccg
     1321 aacaggccac cgccgttggc gcaattcata gcgcggcaga tactttttct gcctggctgc
     1381 tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg gaaaacgtgc
     1441 tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca ccgcaaacta
     1501 tcgcccgttt tcgtgaacgt tttaatgccc ccgtggaagt tctgcattcc ggcctgaacg
     1561 acagcgagcg gctttctgcg tggttgaaag cgaaaaatgg cgaggcggcg attgtgatcg
     1621 gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc attgatgaag
     1681 agcacgacag ctcctacaag cagcaggaag gctggcgcta tcatgcccgc gacctggcgg
     1741 tgtatcgtgc gcacagcgag caaatcccga ttattcttgg ctccgcaacg cccgcgctgg
     1801 aaacgttatg caacgtccag cagaaaaaat accgcctgct gcgcctgacc cgtcgggcag
     1861 ggaatgctcg tccggcaatt caacatgtgc tggatttaaa aggtcagaag gtgcaggcag
     1921 gtctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccaac aatcaggtta
     1981 tcctctttct taatcgtcgg ggttttgcgc ctgcgctgct gtgccacgac tgcggctgga
     2041 tagccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa cagcatttgc
     2101 gctgtcacca ctgtgacagt cagcgtccgg tgccgcgcca gtgcccttcc tgcggttcca
     2161 cgcacctggt ccccgtgggg ctgggcaccg aacagcttga acagacgctt gcgccgatgt
     2221 tccccggcgt gcccatttca cgtatcgacc gcgataccac cagccgcaaa ggggcactgg
     2281 aacagcaact ggcggaagtg catcgcggcg gcgcgaggat tttgatcggc acacaaatgc
     2341 tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat gtggacggcg
     2401 cgctgttttc tgccgatttt cgctcggcag aacgtttcgc ccagctttac acccaggtcg
     2461 ccggtcgtgc cgggcgtgcg ggtaaacagg gcgaagtggt gctgcaaacg caccatccgg
     2521 aacatcctct gttgcaaacg ttgctctata aaggctacga cgcctttgcc gaacaggcgc
     2581 tggctgagcg acgcatgatg cagctgccgc cgtggaccag ccatgtgatt gtgcgtgcgg
     2641 aagatcataa caatcagcac gcgccgctgt tcctgcaaca actgcgtaat ctgatccttt
     2701 ccagcccgct ggcagacgac aaactgtgga ttctcggccc ggttccggct ctggcaccta
     2761 agcgtggtgg ccgctggcgc tggcagatct tgttgcagca cccttcccgc gtgcgcttgc
     2821 aacacatcat tagtggtacg ctggcgctta ttaatacaat accggattcc cgcaaggtga
     2881 aatgggtgtt ggatgtcgat cccatcgaag gctaatgcgc aaacgttgcg aggcggatcg
     2941 aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc acgcgctatc
     3001 tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac gtaccaggcg
     3061 aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag acgttgccct
     3121 caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg ataaagtctc
     3181 ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt taccgcagcc
     3241 tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg tcccggatat
     3301 ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg caaatcacgg
     3361 atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa cctttatcga
     3421 tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggttcaaggc tgccgtttga
     3481 tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg aatttgcacc
     3541 ggaactggag ctgcctaccg ttcatatcga caatctgacc gccgcatttg atgcagtaaa
     3601 ttatttatat gagcaagggc ataaacggat tggctgtata gccggtcccg aagagatgcc
     3661 actgtgtcac taccgcctgc aaggctatgt tcaggcgctg cgtcgctgcg gaattatggt
     3721 tgatccacaa tacatcgccc gtggcgactt caccttcgaa gccggaagca aagcaatgca
     3781 acagctgctt gatcttccac aaccgcctac tgcagtcttc tgccatagcg atgtgatggc
     3841 gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag acctttccat
     3901 aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga caaccatcgc
     3961 gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc aaatgcaggg
     4021 gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc ggggatcaac
     4081 acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag gcccgccgct
     4141 taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg gcacaacgag
     4201 attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc tcacggaaaa
     4261 agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc gccgttcttg
     4321 tgacctttat cggtggtctg tacttcatta cgcatcacaa gaaagaagag tccgagacgc
     4381 tgcaaagcca gaaagtgacc ggaaacggac taccaccaaa accagaagaa cgctggcgct
     4441 acattaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa ccttctgccg
     4501 gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt cttgaacaaa
     4561 tgcaggctga tatgcgccag cagccaacgc agctggttga ggttccgtgg aatgaacaga
     4621 cgccggaaca gcgtcagcaa acgctacaac gccagcgtca ggcgcagcaa ttagcggagc
     4681 agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag cagacgcgta
     4741 cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccagct tctactcagc
     4801 agccgtacca ggatctgctg caaacccctg cgcacacgac cgcgcagtcg aagccgcagc
     4861 aagctgcacc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag aaaaaagacg
     4921 aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca gagacggtac
     4981 gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat ggctggaatc
     5041 gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc aatcggttga
     5101 agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac cctcaaaatc
     5161 ccccccatct ataattgcat tatgcctcgt acttttgtac ggggtttgta ctctgtattc
     5221 gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg gccatgtggt
     5281 catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca acgtgaaaaa
     5341 ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta ctgcggatgc
     5401 ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc atctggttaa
     5461 agccgccgtt gagctggcaa aagactggcg taccgatcgc atgctgcgca aacttgaagc
     5521 actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg gtgacgtggt
     5581 gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc aggctgcggc
     5641 gcgcgcgctg ttagaaaaca ctgaacttag cgcccgtgaa attgctgaaa aggcgttgga
     5701 tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag aattaagcta
     5761 caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca gcgaactgga
     5821 taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc tgcgtaaccg
     5881 ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga aaaatatcct
     5941 gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg ctaagctggc
     6001 gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtgggct atgtcggtaa
     6061 ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg tacgcgtcca
     6121 ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta ttctcgacgt
     6181 gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg aaccgtccgc
     6241 tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca aagaaatcga
     6301 gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccag gcatggaaga
     6361 gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc aaaaagcgcg
     6421 taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg cgaaactggt
     6481 gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg ggatcgtgtt
     6541 tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg atgtttctcg
     6601 tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt ccaccaaaca
     6661 cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc agattgcgaa
     6721 accgtctgac ctaatcccgg aactgcaagg tcgtctgcca atccgcgttg aactgcaggc
     6781 actgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta tcaccgtgca
     6841 gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact ccggtattaa
     6901 acgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg gtgctcgtcg
     6961 tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca gcgatttaag
     7021 cggtcaaaat atcactattg acgcagatta tgtgagcaaa catctggatg cgttggtggc
     7081 agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca tcattgtttg
     7141 atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac aaattagccg
     7201 aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct ttgctgcaat
     7261 tatcgtcggg acggcgctgg catggtggca aggtcatttt gatccgctgg tcgccctgct
     7321 ggcactgatt accgccgggc tattacagat cctttctaac ctcgccaatg attacggcga
     7381 tgcggtaaaa ggcagcgata gacctgaccg cattgggccg ctacgcggca tgcaaaaagg
     7441 ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc tcatctgtct
     7501 ctccgggctg gcactggttg cagtggcgtg ccatacgctg gctgattttg tcggtttcct
     7561 gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca atcgtcctta
     7621 tggttatatc ggtctggggg atatttccgt actggttttc tttggctggt taagcgtcat
     7681 ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc cggcgaccgc
     7741 atgcggcctg ctggcaacgg cagtactgaa cattaataac ctgcgtgata tcaatagcga
     7801 ccgagaaaac ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg cgcgtcgtta
     7861 tcatgcctgc ctgctgatgg gctctctggt atgtctggcg ctgtttaatc tcttttcgct
     7921 gcatagcctg tggggctggc tgttcctgct ggcggcaccg ttgctggtga agcaagcccg
     7981 ttatgtgatg cgggaaatgg acccggtggc gatgcgaccg atgctggaac gcactgtcaa
     8041 gggggcgtta ctgactaacc tgctgtttat tttagggata ttcctaagcc agtgggcagc
     8101 ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca tagcgcgata
     8161 tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac ttccgagctt
     8221 tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa ctttggcgga
     8281 cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa cgggttgctg
     8341 tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg cggtggttct
     8401 gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa tgaatgggaa
     8461 ggtctggtta tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt ggatatcggc
     8521 atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg cgaaagcgat
     8581 gtccgcgtca attttggcgg tgtaaccttc ttctccggcg accatcttta tgccgacaat
     8641 accgggatta ttctttcaga agatccgctg gatattgaat gataagaaag gcaccgcaag
     8701 gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc gaaagatgaa
     8761 gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct gccagccgtt
     8821 ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat gctgggcatt
     8881 ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt ccatctgcaa
     8941 cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa acacttctaa
     9001 tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaaat cctcgtcccg
     9061 attaccggtg acgccttaat aaatacgagc gcactttagt tagctccgat tgtatgaagc
     9121 cgcgccatcg ctgtccagcg gcacgccttg cagattacgg tttgccacac ttttcatcct
     9181 tctcctggtg acataatcca catcaatcga aaatgttaat aaatttgttg cgcgaatgat
     9241 ctaacaaaca tgcatcatgt acaatcagat ggaataaatg gcgcgataac gctcatttta
     9301 tgacgaagca cacacatttt aagttcgata tttctcgttt ttgctcgtta acgataagtt
     9361 tacagcatgc ctacaagcat cgtggaggtc cgtgactttc acgcatacaa caaacattaa
     9421 ctcttcagga tccgattatg agtcaaacat caaccttgaa aggccagtgc attgctgaat
     9481 tcctcggtac cgggttgttg atttttttcg gtgtgggttg cgttgcagca ctaaaagtcg
     9541 ctggtgcgtc ttttggtcag tgggaaatca gtgtcatttg gggactgggg gtggcaatgg
     9601 ctatctacct gaccgcaggg gtttccggcg cgcatcttaa tcccgctgtt accattgcat
     9661 tgtggctgtt tgcctgtttc gacaagcgca aagttattcc ttttatcgtt tcacaagttg
     9721 ccggcgcttt ctgtgctgcg gctttagttt acgggcttta ctacaattta tttttcgact
     9781 tcgagcagac tcatcacatt gttcgcggca gcgttgaaag tgttgatctg gcaggcactt
     9841 tctctactta ccctaatcct catatcaatt ttgtgcaggc tttcgcagtt gagatggtga
     9901 ttaccgctat tctgatgggg ctgatcctgg cgttaacgga cgatggcaac ggtgtaccac
     9961 gcggcccttt ggctcccttg ctgattggtc tactgattgc ggtcattggc gcatctatgg
    10021 gcccattgac gggttttgcc atgaacccag cgcgtgactt cggtccgaaa gtctttgcct
    10081 ggctggcggg ctggggcaat gtcgccttta ccggcggcag agacattcct tacttcctgg
    10141 tgccgctttt tggccctatc gttggcgcga ttgtaggtgc atttgcctac cgcaaactga
    10201 ttggtcgcca tttgccttgc gatatctgtg ttgtgaaaga aaaggaaacc acaactcctt
    10261 cagaacaaaa agcttcgctg taatatgact acgggacaat taaacatgac tgaaaaaaaa
    10321 tatatcgttg cgctcgacca gggcaccacc agctcccgcg cggtcgtaat ggatcacgat
    10381 gccaatatca ttagcgtgtc gcagcgcgaa tttgagcaaa tctacccaaa accaggttgg
    10441 gtagaacacg acccaatgga aatctgggcc acccaaagct ccacgctggt agaagtgctg
    10501 gcgaaagccg atatcagttc cgatcaaatt gcagctatcg gtattacgaa ccagcgtgaa
    10561 accactattg tctgggaaaa agaaaccggc aagcctatct ataacgccat tgtctggcag
    10621 tgccgtcgta ccgcagaaat ctgcgagcat ttaaaacgtg acggtttaga agattatatc
    10681 cgcagcaata ccggtctggt gattgacccg tacttttctg gcaccaaagt gaagtggatc
    10741 ctcgaccatg tggaaggctc tcgcgagcgt gcacgtcgta gtgaattgct gtttggtacg
    10801 gttgatacgt ggcttatctg gaaaatgact cagggccgtg tccatgtgac cgattacacc
    10861 aacgcctctc gtaccatgtt gttcaacatc cataccctgg actgggacga caaaatgctg
    10921 gaagtgctgg atattccgcg cgagatgctg ccagaagtgc gtcgttcttc cgaagtatac
    10981 ggtcagacta acattggcgg caaaggcggc acgcgtattc caatctccgg gatcgccggt
    11041 gaccagcagg ccgcgctgtt tggtcagttg tgcgtgaaag aagggatggc gaagaacacc
    11101 tatggcactg gctgctttat gctgatgaac actggcgaga aagcggtgaa atcagaaaac
    11161 ggcctgctga ccaccatcgc ctgcggcccg actggcgaag tgaactatgc gttggaaggt
    11221 gcggtgttta tggcaggcgc atccattcag tggctgcgcg atgagatgaa gttgattaac
    11281 gacgcctacg attccgaata tttcgccacc aaagtgcaaa acaccaatgg tgtgtatgtg
    11341 gttccggcat ttaccgggct gggtgcgccg tactgggacc cgtatgcgcg cggggcgatt
    11401 ttcggtctga ctcgtggggt gaacgctaac cacattatac gcgcgacgct ggagtctatt
    11461 gcttatcaga cgcgtgacgt gctggaagcg atgcaggccg actctggtat ccgtctgcac
    11521 gccctgcgcg tggatggtgg cgcagtagca aacaatttcc tgatgcagtt ccagtccgat
    11581 attctcggca cccgtgttga gcgcccggaa gtgcgcgaag tcaccgcatt gggtgcggcc
    11641 tatctcgcag gcctggcggt tggcttctgg cagaacctcg acgagctgca agagaaagcg
    11701 gtgattgagc gcgagttccg tccaggcatc gaaaccactg agcgtaatta ccgttacgca
    11761 ggctggaaaa aagcggtcaa acgcgcgatg gcgtgggaag aacacgacga gtaatgtaaa
    11821 tgccggatga agcgtttatg ccgcatccgg tagtcctgaa acgtgcgggg gcaaccccgc
    11881 acacatcaat aatcccaccc ttcccctgtg ctacacttcg cgccattcct tactgcttag
    11941 agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa tctgcggcgc
    12001 tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc gcggcggtaa
    12061 acgccatgcg tattatgctc aaccaggtca acattgacgg caccatcgtc attggtgaag
    12121 gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact ggtcgcggcg
    12181 acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg atgggccagg
    12241 ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg cttcctcaat gcgccggata
    12301 tgtatatgga gaagctgatt gtcgggccgg gagccaaagg caccattgat ctgaacctgc
    12361 cgctggcgga taacctgcgc aatgtagcgg cggcgctcgg caaaccgttg agcgaactga
    12421 cggtaacgat tctagctaaa ccacgccacg atgccgttat cgctgaaatg cagcaactcg
    12481 gcgtacgcgt atttgctatt ccggatggcg acgttgcggc ctcaattctc acctgtatgc
    12541 cagacagcga agttgacgtg ctgtacggta ttggtggcgc gccggaaggc gtagtttctg
    12601 cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg cgtcatgacg
    12661 tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca cgctgcaaag
    12721 cgatgggcat cgaagccggt aaagtattgc gcctgggcga tatggcgcgc agcgataacg
    12781 tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt agccgcaaag
    12841 gcaatatcgc gactaccgaa acgctgctga tccgcggcaa gtcacgcacc attcgccgca
    12901 ttcagtccat ccactatctg gatcgcaaag acccggaaat gcaggtgcac atcctctgat
    12961 tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt tcggagaacg
    13021 aagataaggc aagtcaatca aaacaggaga aaaacatggc tgattgggta acaggcaaag
    13081 tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac gcccccgtgc
    13141 ttccgtttac cgccgggcaa ttcaccaagc ttggccttga aatcggcggc gaacgcgtcc
    13201 agcgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt tacctggtca
    13261 ccgtccccga tggcaaatta agcccacgat tggcggcact gaaaccaggc gatgaagtgc
    13321 aggtggttag cgaagcagct ggcttctttg ttctggatga agtaccagat tgcgaaacgc
    13381 tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg caactaggca
    13441 aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat gccgccgact
    13501 taagctattt gccactgatg caagaactgg aaaaacgcta cgaaggcaaa ctgcgcattc
    13561 agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cggacggata ccggcattaa
    13621 ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat gaataaagaa accagccatg
    13681 tgatgctgtg cggcaatcca cagatggtgc gcgatacaca acagttgctg aaagagaccc
    13741 ggcagatgac gaaacattta cgtcgccgac cgggccatat gacagcggag cattactggt
    13801 aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcatct tattgatcat
    13861 cccgcgatat gctggcatca aacggttgat aaatgagtga tgatgaccac agacaaggag
    13921 gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc cgcgttcaat
    13981 acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct tgttatccga
    14041 cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac ccggatacaa
    14101 ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat gtctggcgag
    14161 ctccagagct ttattcacca gtaaggcatc ttcttcattc ccggaaattg ccacgccaat
    14221 gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa cgaacgcagt
    14281 gataactcaa aaataatcat ctcggcctgg catgagaaag tgaaggccgc atcaagctca
    14341 acttgcccct ccagatcctt aatcttgtag gccacgctgg ccccttcatc tacagaggat
    14401 gcagcggcgg caagctcttt cagtttggtc ataaaagact ctgcttcttc gcgggtggca
    14461 aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc gatatcaatc
    14521 gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct ctgtatattc
    14581 gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac aaacaagtaa
    14641 tgatcttatg gattaccatt ttttttgata ttgcttaagg tgaagagttc agaaggattt
    14701 tccgggtatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata gcagtaagag
    14761 atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat cctgagtatc
    14821 tttaccatat ttattttcgc cttgtgtgcc aacaaacgca ccaagatcga taagcatcat
    14881 caccagaatc aacgtcggga caaaacaccc caccgcccat tgccagacac ccggtaaaat
    14941 cgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc atgcgccgga
    15001 acgcccgcga tcatgcaagc gcttaacagt tactgccgct gttggccaaa gcaagcacac
    15061 aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg agaaaagcac
    15121 cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc caatacgccc
    15181 tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt tgtcttttcc
    15241 aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc agtcataaca
    15301 aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt ctgcattaac
    15361 cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc cttatctgct
    15421 ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact ttaacagcca
    15481 gaatcctagc ctgccactga acgaatttcg tgccatcgac agcagtcccg acaaagccaa
    15541 tctcactcgt gctgcaagta aaattaacga gaacttgtat gcttctacag cgctggagcg
    15601 cggtacctta aaaatcaaaa gcattcaaat gacctggcta cccatccagg ggccagagca
    15661 aaaagcagcg aaagcgaaag ctcaggaata catggcagca gtgatccgca cactcacccc
    15721 attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctgctaacgg cggggaaaaa
    15781 caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg acaacggcga
    15841 aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat cgcttgaagg
    15901 tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac tgtttcacag
    15961 acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg gagaattaaa
    16021 atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca catggttcac
    16081 gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc ggttgcaatc
    16141 gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag ctgaaggcag ccacatcatg
    16201 ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga aacctctgct
    16261 gctatgctga aagacatcgg cgcacagtac atcatcatcg gccactctga acgtcgtact
    16321 taccacaaag agtctgacga actgatcgcg aaaaaattcg cggtgctgaa agagcatggt
    16381 ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg caaaactgaa
    16441 gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc ggcattcgaa
    16501 ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc tgcaactccg
    16561 gctcaggcac aggctgttca caaattcatc cgtgaccaca tcgctaaagt tgacgctaac
    16621 atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa cgctgcagaa
    16681 ctgtttgctc agccagacat cgacggcgcg ctggttggcg gtgcgtctct gaaagctgac
    16741 gctttcgcag taatcgttaa agctgcagaa gcggctaaac aggcttaagt ctgacaggtg
    16801 ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca aaatctcaca
    16861 ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat ttcgttgtgt
    16921 cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt agcttcccat
    16981 atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc gttgtaccag
    17041 ttcgctttcc gttacccgac gcgccaggta ttcatgcccg cgcaaaccac ctggcagtgg
    17101 caaccagcgg ctgctgatgt tcgccagatt gttatccagt tgttcgcgca catcaggacg
    17161 aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg agttgatcgc
    17221 caaagaaacc gcgcgatcgg gaaccggctg gccgtacttt ttgctcataa aatcacgcgc
    17281 ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag gactttcagt
    17341 accgttaata cgatacgttg gcatcaacag atattgcagt gggccattaa gatcttttaa
    17401 aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt gattttgctg
    17461 ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact cttcaccggt
    17521 taatttccag taaccaatac cggcagccac aacggcgata actatcatca ccaaaaaaag
    17581 aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag ggtaacgcaa
    17641 aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg ggtttgataa ttatcctggg
    17701 gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc tctttttgcg
    17761 ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt ggaaacgcat
    17821 tttcgtactt tttcgctact tcagcgtcgc gcggacggta gtagtttttc gcggcaattt
    17881 cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct ttggtgcctt
    17941 ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata gactcactcg
    18001 gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga gcttcgtttt
    18061 cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa ggtgttagtg gagccgcgcg
    18121 cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc tgtgcttttg
    18181 cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc agccaggtag ttccagcgtg
    18241 cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta atcagatcgt
    18301 tccagtcatg gatctgcttc ggattgccct tacgcaccag gaaaacaatg gtggaagtgt
    18361 acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc ccgcgttccg
    18421 caattgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca ataccgttga
    18481 ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg acgcatcacc acgttatcac
    18541 cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac aattcgcgcg
    18601 ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg gttgccgcca
    18661 gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat gttgtgttgt
    18721 gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa gattggctat
    18781 aacttgcggg tatatgttga gggattaaaa aggcggaaga aatccgcctc atattgctga
    18841 caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc tacaaaagtt
    18901 tgcaaattca ataaattgca gaattcatgt aggcctgata agcgaagcgc atcaggcatt
    18961 tttgcttctg tcatcggttt caggctaaag gaatctgcct ttttccgaaa tcattaatac
    19021 agttttttcg cgcagtccag ccagtcacct ttgaacggac gcttcatgtt ttcgatagcg
    19081 tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacacaacg accgccgtaa
    19141 cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat acggtcgtaa
    19201 ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt ttcacgaccg
    19261 gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc ggtaatcgcc
    19321 acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac caggtcttca
    19381 cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat ggccgcagcc
    19441 aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg ctggtgagaa
    19501 gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc agtgaagaaa
    19561 ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag accgatgcac
    19621 gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc gccgataacc
    19681 accagcgcgt cgataccacg ttttttcagg ttttcgatag ccacggcgcg gatgttctcg
    19741 tcgcggaatt ccgggaaacg cgcagaaccg aggaacgtac cgccacggtt gatcatgtca
    19801 gacacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag atagccgtca
    19861 taaataccca ttacttccag accttctgtc agcgcagaac gaacaacccc gcgaattgcg
    19921 gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt aatcatgact
    19981 acctctgaac tttggaatgc aaaatgaaat ctgttgccgg aagtcttctt gcacatcgaa
    20041 gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg attcaggtca
    20101 ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta tactgattat
    20161 gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg atggataatt
    20221 acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc caccatatgt
    20281 gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa gcgggtcggc
    20341 cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga agtcacgata
    20401 tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga ctgtaccgcc
    20461 tcatatccca tgcgtaacgc gctatataaa atatagatgc cgattcccaa tgcaaacaga
    20521 gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat tgcgccgttc
    20581 atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct ttgcgtccgg
    20641 cgcaccaccc aacgctgaaa cgagacaagg ataatcgtac aaattagcgc cacaattgtc
    20701 acgataaccc cgacgcctgg atctatcatc ggtgttggag atatcagatg ttgaataccc
    20761 gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag cgccgcgagg
    20821 gactctgctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag ggaatatcgc
    20881 accaccagta aattcgtcaa cgacgcgccg atatccacca gcgaatccac cagcgcggcg
    20941 agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa tagcaacgaa
    21001 gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga ttgattcata
    21061 aatactcccg ctatcaactg acgctagtat aacggaagca aatcatctgc aatgcattaa
    21121 gcagcaggca aattgaggat aaaaaaaacc ccacatcatg tgggggaaga cagggatggt
    21181 gtctatggca aggaaaacag ggtttactac tgggaacgtg agttgctact actcaatagc
    21241 ttcaacgatg aacttttttg ccattgcgtc acgtcacgca actgctccat tcgttgttga
    21301 tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg
    21361 acttttgcca tctcaacctg acgagcaatt tgctcattcg ccattttttc tgcctgtgcg
    21421 cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc gatgcattgt ctccagttcg
    21481 ctaacattaa caggaggctg ttcgtgccgg gcctgttgca taagatctcg catctgctga
    21541 cgctgatgtt cggttaaact tatgccgtcg aacatatggc tctgcgtact gcgctgcgta
    21601 ctgcgctgcg taagttcttc acccggatgc cagttatcgc ctgaaccgac ttcagcagcg
    21661 tggcttaatg aactgactgc cagcgttgaa gccatgacgg cagcggtaac tatgcgcatc
    21721 atttgctccc aaaatctttc tgtcgcgatt caacgataga gagtttacga ttcaggctgc
    21781 aaacatgcgt cagggggtgt aaaacaacgt aaagtcatgg attagcgacg tctgatgacg
    21841 taatttctgc ctcggaggta tttaaacaat gaataaaatc ctgttagttg atgatgaccg
    21901 agagctgact tccctattaa aggagctgct cgagatggaa ggcttcaacg tgattgttgc
    21961 ccacgatggg gaacaggcgc ttgatcttct ggacgacagc attgatttac ttttgcttga
    22021 cgtaatgatg ccgaagaaaa atggtatcga cacattaaaa gcacttcgcc agacacacca
    22081 gacgcctgtc attatgttga cggcgcgcgg cagtgaactt gatcgcgttc tcggccttga
    22141 gctgggcgca gatgactatc tcccgaaacc gtttaatgat cgtgagctgg tggcacgtat
    22201 tcgcgcgatc ctgcgccgtt cgcactggag cgagcaacag caaaacaacg acaacggttc
    22261 accgacactg gaagttgatg ccttagtgct gaatccaggc cgtcaggaag ccagcttcga
    22321 cgggcaaacg ctggagttaa ccggtactga gtttaccctg ctctatttgc tggcacagca
    22381 tctgggtcag gtggtttccc gtgaacattt aagccaggaa gtgttgggca aacgcctgac
    22441 gcctttcgac cgcgctattg atatgcacat ttccaacctg cgtcgtaaac tgccggatcg
    22501 taaagatggt cacccgtggt ttaaaacctt gcgtggtcgc ggctatctga tggtttctgc
    22561 ttcatgatag gcagcttaac cgcgcgcatc ttcgccatct tctggctgac gctggcgctg
    22621 gtgttgatgt tggttttgat gttacccaag ctcgattcac gccagatgac cgagcttctg
    22681 gatagcgaac agcgtcaggg gctgatgatt gagcagcatg tcgaagcgga gctggcgaac
    22741 gatccgccca acgatttaat gtggtggcgg cgtctgttcc gggcgattga taagtgggca
    22801 ccgccaggac agcgtttgtt attggtgacc accgaaggcc gcgtgatcgg cgctgaacgc
    22861 agcgaaatgc agatcattcg taactttatt ggtcaggccg ataacgccga tcatccgcag
    22921 aagaaaaagt atggccgcgt ggaactggtc ggtccgttct ccgtgcgtga tggcgaagat
    22981 aattaccaac tttatctgat tcgtccggcc agcagttctc aatccgattt cattaactta
    23041 ctgtttgacc gcccgctatt actgctgatt gtcaccatgt tggtcagtac gccgctgctg
    23101 ttgtggttgg cctggagtct ggcaaaaccg gcgcgtaagc tgaaaaacgc tgccgatgaa
    23161 gttgcccagg gaaacttacg ccagcacccg gaactggaag cggggccaca ggaattcctt
    23221 gccgcaggtg ccagttttaa ccagatggtc accgcgctgg agcgcatgat gacctctcag
    23281 cagcgtctgc tttctgatat ctctcacgag ctgcgcaccc cgctgacgcg tctgcaactg
    23341 ggtacggcat tactgcgccg tcgtagcggt gaaagcaagg aactggagcg tattgaaacc
    23401 gaagcgcaac gtctggacag catgatcaac gatctgttgg tgatgtcacg taatcagcaa
    23461 aaaaacgcgc tggttagcga aaccatcaaa gccaaccagt tgtggagtga agtgctggat
    23521 aacgcggcgt tcgaagccga gcaaatgggc aagtcgttga cagttaactt cccgcctggg
    23581 ccgtggccgc tgtacggcaa tccgaacgcc ctggaaagtg cgctggaaaa cattgttcgt
    23641 aatgctctgc gttattccca tacgaagatt gaagtgggct ttgcggtaga taaagacggt
    23701 atcaccatta cggtggacga cgatggtcct ggcgttagcc cggaagatcg cgaacagatt
    23761 ttccgtccgt tctatcggac cgatgaagca cgcgatcgtg aatctggcgg tacaggattg
    23821 gggctggcga ttgttgaaac cgccattcag cagcatcgtg gctgggtgaa ggcagaagac
    23881 agcccgctgg gcggtttacg gctggtgatt tggttgccgc tgtataagcg gagttaaact
    23941 ccgcatttgt aggcaggata aggcgtttac gccgcatccg gcatttgagc aggatgcctg
    24001 atgcgacgct gatagcgtct tatcaggcct acactccggg ggttattttc cccacaaccg
    24061 gcgggaaaaa tcttcgatct tgccgctcag tcggcgcttt tgcatcgtcc gcgtccagct
    24121 tttcgataac ccggcggcgg agagtaaacg gtgatactga tcgtcatcaa acggcatatg
    24181 ccatgcgatg gcggcagcct cctgcacggt cacatcgctg acacgggaaa ccaactccag
    24241 cggcgcgtcc gcagacactt tccctggcgc tatcacgcta tacaaccacc ccaccttacc
    24301 tgtgttttgc atcaactgcg ctatatcgct gatatcaaaa tggtaattga gtttatagca
    24361 aggcgagcgc ggctggctga cctgaattaa tgcctctccc cagcggaaaa tatcgcccat
    24421 ataaacatta ctttccgtca ggccgtcggt tgagaggttt tcaccaaacg caggcgcaac
    24481 aaacaactcc gcctgttccg gaaattcccg cgcccagtag agataatgct cacgaggata
    24541 atgacacagc gctctgtctg gcccaccatg aacctttttc tccgcctgct cgtcaccttc
    24601 cagccccagc tccgtcagca tcaactcacc atcaacctgg attttggcaa ttgcgctggg
    24661 tttgccttcg ggataagcct gaatcttgcc tgtgtataca tcaaccggat atcgcatctg
    24721 ttccctctcc acgagaaatt tccccattag accatattta ttttatgtca ggcgtgctgt
    24781 tttcgcggta agtatccagg caggaactca atagcaaacc ggacgaacgt tagttcatcc
    24841 ggctgtgggg attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg
    24901 ctctggcttt gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca
    24961 caatgaccgc cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca
    25021 ccatttctgc aatcagcata ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa
    25081 ttccggcagt gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg
    25141 tcacgataat tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag
    25201 tcaaatcaat ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt
    25261 tgctgaaaaa ttctcgtaat tcagggtcaa ggttccccag ggcaaagccc accaggaacg
    25321 gtaatactgc gccgacgaaa acatgcggtt caaacgaggc aatccctgca gtgcccagaa
    25381 taatcatcgt catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt
    25441 cttttgtgcc gtactgctgc atgatggaag cgtaaagtcc gccattggtc atatccatcg
    25501 ccgccaccag cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg
    25561 gaataatgcg cgaggcaatt gccgcaacca cccacgcgac ggcaattttt gttaccacca
    25621 gcgtaccgga tttacgcaat accgttcccg tcgcgcttaa ttttattgac gcccccatgc
    25681 aaaaaaacca caccgccaga atggacaccg taccggtaat catcccgttg gtgaatgatc
    25741 caaaatattt ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga
    25801 cgagcatcat ccccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc
    25861 tgtcggcata tttttaaggg ctgatgcgta attcctcaat ggagcatcag tgttgatgga
    25921 aacgaagata gacattcatt taatcaataa aagtgatcaa aaccacatca attgaaacgc
    25981 tgttttattt ttatcggatc attgttttta ttttgaaatt gatcacaaaa aaaccaccgc
    26041 cgttggcgat ggttcatttt ttaagctgat atgcggcgta aacgcctcat tgcagcaggc
    26101 ggcaaatgat tattttttcg ccgcgaaacg tgccgctgct tcatcccagt tcaccacgtt
    26161 ccagaactct ttgatgtagt ccggacggcg gttctggaat ttcaggtagt aagcatgttc
    26221 ccacacatcc aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt cacccatcag
    26281 cggggaatcc tggttagcag tagaaaccac cgccagttta tcgcctttca gcaccagcca
    26341 tgcccagccg gaaccaaagc gggaagctgc cgctttctca aattctgctt tgaagttatc
    26401 aacggagccg aagtcacgtt cgatagccgc tttcaggtca ccctgtaagg tggtgccttt
    26461 tttcagacct ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac
    26521 ggttttcttg tctgctggca gctggtccag tttggtgatc agctcttcaa ccggcaggtt
    26581 ggcaaattct ggcaggcttt ccagcgccgc gttggcgttg ttgacgtagg tctgatggtg
    26641 tttggtgtgg tggatttcca tggtctgctt atcgaagtgc ggttccaggg catcgtaagc
    26701 atacggcagg gatggcaggg tatagctcat attcatctcc agtattgtcg ggcggccgat
    26761 tgttaatgcg cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta
    26821 tcaatgccgt acttttcgta agggtatggt tttgcaggaa aattcccgag atgtgaagca
    26881 aatcacccac ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg
    26941 ttaatcgaca gcacgatttt tacactcatc tcgtcggaga tgtgacgcga cgaaaaatga
    27001 tgaggataag aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc
    27061 ggccagtgca gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac
    27121 catgtggtca gttggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct
    27181 gttactgccg aatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt
    27241 ttttctgttc ggcgctatgt gggggatcgg taatatcaac tacggcctga ccatgcgtta
    27301 tctcggcatg tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct
    27361 gatgacgcca attatcaacg gcaatttcga tgttctgctt aataccgaag gcggacgcat
    27421 gacgttgctc ggcgttctgg tggcgctgat tggtgtaggg attgtgactc gcgccgggca
    27481 gttgaaagag cgcaaaatgg gcattaaagc cgaagagttc aacctgaaaa aagggctggt
    27541 gctggcggtg atgtgcggca ttttctctgc cggtatgtct tttgcgatga acgccgcaaa
    27601 accgatgcat gaagccgctg ccgcactggg cgtcgatcca ctgtatgtcg ctctgccaag
    27661 ctatgtcatc atcatgggcg gcggcgcgat catcaacctc ggtttctgct tcattcgtct
    27721 ggcaaaagtg aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat
    27781 tcacaatgtg ttactctcag cactgggcgg gttgatgtgg tatctgcaat tctttttcta
    27841 tgcctggggc cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat
    27901 gagtttctat gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatgc
    27961 aggtcgccgt ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgccaa
    28021 catcgtcggc atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct
    28081 gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc
    28141 acattcggtc gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg
    28201 acacactctg acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg
    28261 ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg
    28321 acttttcagg ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt
    28381 tggcggcaac gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa
    28441 cagcaactca gccatttcgt tagcaaacga cacatgctga ctactttcat gctcaagctg
    28501 accgataacc tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc
    28561 tgcgctggcg ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc
    28621 cgggcaataa ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc
    28681 gtcagcatga atgtaaaaga gatcgccacg ggtaatgcga taagggcgat cgttgagtac
    28741 atgcagacca ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc
    28801 aaaaacatct tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa
    28861 atcatctttg agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag
    28921 ttgattattc gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc
    28981 catcccgtcc ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg
    29041 aaaagtggtt actgtcgctg aatccgcagc gataggcgat gtcagtaacg ctggcctcgc
    29101 tgtgacgtag cagatgtcgg gccttcatca gtcgcaggcg gttcaggtaa cgctgaggcg
    29161 tcagtcccgt ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat
    29221 ccgccacggc atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca
    29281 agttgagacg tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga
    29341 gcagtaattg cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt
    29401 cctgctgttc catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc
    29461 gccagtgaga cggatactgc ccatccagtt cttgtggcag caactgattc agcccggcga
    29521 gaaactgaaa tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat
    29581 gttcatacag atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat
    29641 agggctgccc attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa
    29701 aatcatgatg atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg
    29761 cgttaccaga cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc
    29821 gtcaacacgg cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa
    29881 accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag
    29941 catcacatca ccacaatgaa gcaaattgtg aacatcatca cgttcatctt tccctggttg
    30001 ccaatggccc attttcctgt cagtaacgag aaggtcacga attcaggcgc tttttagact
    30061 ggtcgtaatg aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat
    30121 ctcggcgcat ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg
    30181 acgctgcgcg aaatccatcg ttttaacaat ggcctgcata gccagaacgg ttatgtcacc
    30241 tggaatgtgg atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa
    30301 gggattcgta tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac
    30361 caacagggtc agcgtgtggg cctgcccgtt gcttatcgcg atagccgctc caatggccta
    30421 atggcgcagg cacagcaaca actcggcaaa cgcgatattt atcaacgtag cggcatccag
    30481 tttctgccct tcaatacgct ttatcaactg cgtgcgctga cggagcaaca acctgaactt
    30541 attccacaca ttgctcacgc tctgctgatg ccggattact tcagctatcg cctgaccggc
    30601 aagatgaact gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac
    30661 gactgggacg agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg
    30721 acgcatccgg gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg
    30781 gtcgccgttg ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca
    30841 cgcgctgctt atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca
    30901 tttaccaatg acacggcgct ggcagccaac ataaccaatg aaggcggggc ggaaggtcgc
    30961 tatcgggtgc tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggagcgg
    31021 caaatcaacg atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc
    31081 atcatcaatc ccaatgacga tcgctttatt aatcctgagg cgatgtgcag cgaaattcag
    31141 gctgcgtgtc gggaaacggc gcaaccgatc ccggaaagtg atgctgaact ggcgcagcta
    31201 cgcggtgaag atttctcgca actgcatatt gtcggcggcg gctgccagaa cacgctgctc
    31261 aaccagctat gtgccgatgc ctgcggtatt cgggtgatcg ccgggcctgt tgaagcctcg
    31321 acgctcggca atatcggcat ccagttaatg acgctggatg aactcaacaa tgtggatgat
    31381 ttccgtcagg tcgtcagcac caccgcgaat ctgaccacct ttacccctaa tcctgacagt
    31441 gaaattgccc actatgtggc gcagcttcac tctacacgac agacaaagga gctttgcgca
    31501 tgaccactca actggaacag gcctgggagc tggcgaaaca gcgtttcgcg gcggtgggga
    31561 ttgatgtcga ggaggcgctg cgccaacttg atcgtttacc cgtttcaatg cactgctggc
    31621 agggcgatga tgtttccggt tttgaaaacc cggaaggttc gctgaccggg gggattcagg
    31681 ccacaggcaa ttatccgggc aaagcgcgta atgccagtga gctacgtgcc gatctggaac
    31741 aggctatgcg gctgattccg gggccgaaac ggcttaattt acatgccatc tatctggaat
    31801 cagatacgcc agtctcgcgc gaccagatca aaccagagca cttcaaaaac tggggtgaat
    31861 gggcgaaagc caatcagctc ggtctggatt ttaacccctc ctgcttttcg catccgctaa
    31921 gcgccgatgg ctttacgctc tcgcatcccg acgacagcat tcgccagttc tggattgatc
    31981 actgcaaagc cagccgtcgc gtttcggcct attttggcga gcaactcggc acaccatcgg
    32041 tgatgaacat ctggatcccg gatggtatga aagatatcac cgttgaccgt ctcgccccgc
    32101 gtcagcgtct gctggcagca ctggatgagg tgatcagcaa gaagctaaac cctgcgcacc
    32161 atatcgacgc cgttgagagc aaattgtttg gcattggcgc agagagctac acggttggct
    32221 ccaatgagtt ttacatgggg tatgccacca gccgccagac tgcgctgtgc ctagacgccg
    32281 ggcacttcca cccgactgaa gtgatttccg acaagatttc cgccgccatg ctgtatgtgc
    32341 cgcagttgct gctgcacgtc agccgtccgg ttcgctggga ctgcgatcac gtagtgctgc
    32401 tggatgatga aacccaggca attgccagtg agattgtgcg tcacgatctg tttgaccggg
    32461 tgcatatcgg ccttgacttc ttcgatgcct ctatcaaccg cattgccgcg tgggtcattg
    32521 gtacacgcaa tatgaaaaaa gccctgctgc gtgcgttgct ggaacctacc gctgagctgc
    32581 gcaagctgga agcggcgggc gattacactg cgcgtctggc actgctggaa gagcagaaat
    32641 cgttgccgtg gcaggcggtc tgggaaatgt attgccaacg tcacgatacg ccagcaggta
    32701 gcgaatggct ggagagcgtg cgggcttatg aaaaagaaat tttgagccag cgtgggtaaa
    32761 cactgccgga tgcggcgcga gcgccttatc cggcctacgg gtcggcaaca gttgtaggcc
    32821 tgataagacg cgacagcgtc gcatcaggca ttgattgccg gatgcggcac gagtgcctta
    32881 tccggcctac aggtcggcaa tagttgtagg cctgataaga cgcgacagcg tcgcatcagg
    32941 cattgattgc cggatgcggc acgagcgcct tatccggcct acgggtcgtg catctgacaa
    33001 caccgaattt acaggaacac agaacatgca aaacattacc cagtcctggt ttgtccaggg
    33061 aatgatcaaa gccaccaccg atgcctggct gaaaggctgg gacgaacgca acggcggcaa
    33121 cctgacgctg cgcctggatg acgccgatat cgcgccttat aaagacaacg tccatgcaca
    33181 accgcgctat atcccgctca gccagcccat gcctttactg gcaaatacac cgtttattgt
    33241 caccggctcc ggcaaattct tccgtaacgt ccagctcgat cctgcagcta acttaggcgt
    33301 cgtaaaagtc gacagcgatg gcgcgggcta ccatattctc tgggggttaa ccaacgaagc
    33361 cgtcccgaca tccgaactcc cggcgcactt tctctcccac tgcgagcgca ttaaagccac
    33421 caacggtaaa gatcgggtga tcatgcactg ccacgccacc aacctgatcg ccctcaccta
    33481 tgtactggaa aacgacaccg cggtctttac tcgccaactg tgggaaggca gcaccgagtg
    33541 tctggtggta ttcccggatg gcgttggcat tctgccgtgg atggtgcccg gcacggacga
    33601 aatcggccag gcgaccgcac aagagatgca aaaacattcg ctggtgttgt ggcccttcca
    33661 cggcgtcttc ggcagcgggt cgacgttgga tgaaacattc ggtttaatcg acaccgcaga
    33721 aaaatcagca caagtattag tgaaggttta ttcgatgggc ggcatgaaac agaccatcag
    33781 ccgtgaagag ttgatagcgc tcggcaagcg ctttggcgtt acaccactag ccagtgcgct
    33841 ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgtc atgcaggtta accccgacgc
    33901 ccacgaagag tatcagcgtc ggcataaccc catctggccg gaactggaag cagtgttgaa
    33961 atctcacggt gcgcataact acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc
    34021 cacggtagag attgaatctg aagaacggtg gaatgcggta gccagtgctg atgtttgcca
    34081 acgttggtgg aaatatatga ccgatgttat gcccgctaac ccggataaca gcccggtgag
//
LOCUS       DAJTNN010000025        32656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTNN010000025 DAJTNN010000000
VERSION     DAJTNN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.025"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..1351
                     /gene="gspE"
                     /locus_tag="OO150_001060"
                     /pseudo
     CDS             1..1351
                     /gene="gspE"
                     /locus_tag="OO150_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1341..2543
                     /gene="hofC"
                     /locus_tag="OO150_001061"
     CDS             1341..2543
                     /gene="hofC"
                     /locus_tag="OO150_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5515283.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(2578..3621)
                     /gene="guaC"
                     /locus_tag="OO150_001062"
     CDS             complement(2578..3621)
                     /gene="guaC"
                     /locus_tag="OO150_001062"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5515284.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(3777..3821)
                     /locus_tag="OO150_001063"
     CDS             complement(3777..3821)
                     /locus_tag="OO150_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515285.1"
                     /translation="MIIKTLKMSARKRN"
     gene            3846..4466
                     /gene="coaE"
                     /locus_tag="OO150_001064"
     CDS             3846..4466
                     /gene="coaE"
                     /locus_tag="OO150_001064"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5515286.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4466..5209
                     /gene="zapD"
                     /locus_tag="OO150_001065"
     CDS             4466..5209
                     /gene="zapD"
                     /locus_tag="OO150_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5515287.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5219..5416
                     /gene="yacG"
                     /locus_tag="OO150_001066"
     CDS             5219..5416
                     /gene="yacG"
                     /locus_tag="OO150_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5515288.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5523..5912)
                     /gene="mutT"
                     /locus_tag="OO150_001067"
     CDS             complement(5523..5912)
                     /gene="mutT"
                     /locus_tag="OO150_001067"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5515289.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(5972..8677)
                     /gene="secA"
                     /locus_tag="OO150_001068"
     CDS             complement(5972..8677)
                     /gene="secA"
                     /locus_tag="OO150_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5515290.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(8739..9251)
                     /gene="secM"
                     /locus_tag="OO150_001069"
     CDS             complement(8739..9251)
                     /gene="secM"
                     /locus_tag="OO150_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5515291.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9482..10399)
                     /gene="lpxC"
                     /locus_tag="OO150_001070"
     CDS             complement(9482..10399)
                     /gene="lpxC"
                     /locus_tag="OO150_001070"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5515292.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(10500..11651)
                     /gene="ftsZ"
                     /locus_tag="OO150_001071"
     CDS             complement(10500..11651)
                     /gene="ftsZ"
                     /locus_tag="OO150_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5515293.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(11712..12974)
                     /gene="ftsA"
                     /locus_tag="OO150_001072"
     CDS             complement(11712..12974)
                     /gene="ftsA"
                     /locus_tag="OO150_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5515294.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(12971..13801)
                     /gene="ftsQ"
                     /locus_tag="OO150_001073"
     CDS             complement(12971..13801)
                     /gene="ftsQ"
                     /locus_tag="OO150_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5515295.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKETAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQLAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(13803..14723)
                     /gene="ddlB"
                     /locus_tag="OO150_001074"
     CDS             complement(13803..14723)
                     /gene="ddlB"
                     /locus_tag="OO150_001074"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5515296.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(14716..16191)
                     /gene="murC"
                     /locus_tag="OO150_001075"
     CDS             complement(14716..16191)
                     /gene="murC"
                     /locus_tag="OO150_001075"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5515297.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16245..17312)
                     /gene="murG"
                     /locus_tag="OO150_001076"
     CDS             complement(16245..17312)
                     /gene="murG"
                     /locus_tag="OO150_001076"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5515298.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17309..18553)
                     /gene="ftsW"
                     /locus_tag="OO150_001077"
     CDS             complement(17309..18553)
                     /gene="ftsW"
                     /locus_tag="OO150_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5515299.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18553..19869)
                     /gene="murD"
                     /locus_tag="OO150_001078"
     CDS             complement(18553..19869)
                     /gene="murD"
                     /locus_tag="OO150_001078"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5515300.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(19872..20954)
                     /gene="mraY"
                     /locus_tag="OO150_001079"
     CDS             complement(19872..20954)
                     /gene="mraY"
                     /locus_tag="OO150_001079"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5515301.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(20948..22306)
                     /gene="murF"
                     /locus_tag="OO150_001080"
     CDS             complement(20948..22306)
                     /gene="murF"
                     /locus_tag="OO150_001080"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5515302.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22303..23790)
                     /gene="murE"
                     /locus_tag="OO150_001081"
     CDS             complement(22303..23790)
                     /gene="murE"
                     /locus_tag="OO150_001081"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5515303.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTVPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(23777..25543)
                     /gene="ftsI"
                     /locus_tag="OO150_001082"
     CDS             complement(23777..25543)
                     /gene="ftsI"
                     /locus_tag="OO150_001082"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5515304.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25559..25924)
                     /gene="ftsL"
                     /locus_tag="OO150_001083"
     CDS             complement(25559..25924)
                     /gene="ftsL"
                     /locus_tag="OO150_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5515305.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(25921..26862)
                     /gene="rsmH"
                     /locus_tag="OO150_001084"
     CDS             complement(25921..26862)
                     /gene="rsmH"
                     /locus_tag="OO150_001084"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5515306.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(26864..27322)
                     /gene="mraZ"
                     /locus_tag="OO150_001085"
     CDS             complement(26864..27322)
                     /gene="mraZ"
                     /locus_tag="OO150_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5515307.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(27924..28928)
                     /gene="cra"
                     /locus_tag="OO150_001086"
     CDS             complement(27924..28928)
                     /gene="cra"
                     /locus_tag="OO150_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5515308.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHLMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29010..29096)
                     /locus_tag="OO150_001087"
     CDS             complement(29010..29096)
                     /locus_tag="OO150_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5515309.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29108..29599)
                     /gene="ilvN"
                     /locus_tag="OO150_001088"
     CDS             complement(29108..29599)
                     /gene="ilvN"
                     /locus_tag="OO150_001088"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5515310.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29602..31326)
                     /gene="ilvI"
                     /locus_tag="OO150_001089"
     CDS             complement(29602..31326)
                     /gene="ilvI"
                     /locus_tag="OO150_001089"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5515311.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPISISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            31445..31489
                     /locus_tag="OO150_001090"
     CDS             31445..31489
                     /locus_tag="OO150_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515312.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(31644..32588)
                     /gene="leuO"
                     /locus_tag="OO150_001091"
     CDS             complement(31644..32588)
                     /gene="leuO"
                     /locus_tag="OO150_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5515313.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7835 a         9174 c         7937 g         7710 t
ORIGIN      
        1 ctgtgtctgc gttatcaggg agtcttgctg gatgccagcg aagaggtggt tcatgttgcg
       61 gtagtcgatg caccttcgca tgagttgctg gacgcattgc atttcgctac caccaaacgt
      121 attgagatca cctgctggac gcgccaacaa atggaaggcc acgccagtcg cacacaacag
      181 acattgcccg tagctgttca ggagaagcat cagtcgaaag cagagttgct gactcgaacg
      241 ttacaatctg cgctggaaca acgtgcgtct gatattcata tcgaaccagc ggacaatgcc
      301 taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat
      361 gccggagtcg cattaaccgc cagattaaaa gtgctgggaa acctggatat tgcggaacat
      421 cgcctgccgc aggacgggca attcactgtc gaactggcag gaaacgccgt ctcatttcgt
      481 attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg
      541 aaccaggcac tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct
      601 catgccttgc aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa
      661 acggtcacgc tttatagtgc cctgcaaaag ctgaataccg ctgacattaa tatttgtagc
      721 gtcgaagatc cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt
      781 gccgggctca cctttcaggg cgttttgcgt gcgttattgc gccaggatcc tgacgtcatc
      841 atgatccgat ggcgaaacgg cagaaattgc cattaaagcc gcgcaaaccg gtcacctggt
      901 gttgtctacc ctacacacta attccacctg cgaaacgctg gtacgtttac agcaaatggg
      961 agtcgcccgc tggatgctct catcagcgct tacgctggta atagcccagc gtctggtacg
     1021 taaattttgc ccacattgtc gccagcagca aggggagccc atccatattc cagacaatgt
     1081 atggccgtcg ccgctgcccc actggcaggc acccggttgt gtacattgct accacggttt
     1141 ttatggtcgt accgccttgt ttgaagtcct gcccattaca ccagccatac gccagcttat
     1201 ttccgctaat accgacgttg aatcgctgga aacgcacgca cgacaggcgg gtatgcgtac
     1261 gctttttgaa aacggctgcc tagccgtgga gcaaggctta accacctttg aagagttaat
     1321 ccgcgtactg gggatgccgc atggcgagta agcaactctg gcgctggcat ggcataaccg
     1381 gcgacggcaa tgcgcaagat gggatgctat gggcagagag ccgtgctttg ctgctcatgg
     1441 cactacagca acagatggtt accccactta gcctgaagcg aatcgccatc aattctgcgc
     1501 agtggcgagg agataaaagc gcggaagtca ttcatcaact ggcgacgcta ctcaaagccg
     1561 ggttaacgct ttctgaaggg ctggcactgc tggcggaaca gcatcccagt aagcaatggc
     1621 aagcgttgct gcaatcgctg gcgcacgatc tcgaacaggg cattgctttt tccaatgcct
     1681 tattaccctg gtcagaggta tttccgccac tctatcaggc gatgatccgc acgggtgaac
     1741 tgaccggtaa gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc
     1801 agttgaccga caaagtgaaa tcagcgttac gttatcccat catcatttta gcgatggcaa
     1861 tcatggtggt tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atctataaga
     1921 ccttcaacac cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg
     1981 gcgaatggag ctggctgctg gtgttgttcg gctttctgct ggcgatagcc aataagttgc
     2041 tgatgcgccg accgacctgg cttatagtgc ggcagaaatt gctgttacgc atcccgatta
     2101 tgggttcact gatgcgggga caaaaactca cgcagatctt tacgattctg gcgctgacac
     2161 aaagtgcagg cattactttt ttgcagggcg tagagagcgt cagagaaaca atgcgctgcc
     2221 cgtactgggt gcaacttctg acacaaatcc agcacgatat cagtaacggt catcccatct
     2281 ggctggcgct aaaaaatacc ggggagttta gcccgctctg tttgcaatta gtgagaacag
     2341 gagaggcatc cggctcgctg gacctcatgt tagacaacct cgcccatcat catcgggata
     2401 acacaatggc gctggcggat aacctcgcag ccttactgga accggcgttg ctgatcataa
     2461 cgggaggaat tatcggtacg ctggtggtgg cgatgtatct gccaattttc catttaggcg
     2521 atgcgatgag tgggatggga taacgctggc gtgttgctcc acaccagcgt tggaagatta
     2581 caggttgttg aagatgcggt tttcttgttc ctgcacacgg ataaatgtgg tgcgcttggt
     2641 cagttctttc aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat
     2701 atcacgcgcg gtattttcaa ccgggcctcg cagcggcagc ttaacggttt taccttctgc
     2761 tgcgcgatat tccgcaacgc cgccaacgtg acgtttcatc gcagactcgg agctcatgcc
     2821 gtagaacagc ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg
     2881 gcccgccagc atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg cttttgcgac
     2941 atcgcccggc gtggtgcagc caccgtcgct gacgatcatt ccgcccagac cgtgcgccgc
     3001 atcggcacat tcgattaccg cagaaagctg cggataaccg acgcctgttt tgacgcgagt
     3061 tgtacaaaca gaacctgggc caatgccaac tttaacgata tcagcaccgg agaggataag
     3121 ctcctcacac atttcaccag tcactacgtt gccagcacaa atggttttgg tcggccacgc
     3181 ttcacgcgct ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacatcaat
     3241 acaaacaaag tttaatgccg ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc
     3301 atcagacgta ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat
     3361 aaacgcttgc cactcttcga cagaatagtg tttatgcaca gcagtcaaaa tatcaaaaga
     3421 agccagcgca gaggccatag aaaatgtacc tacggtgtcc atatttgcgg cgataatcgg
     3481 cacgccggac cagctctgat ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc
     3541 ggaacggctt ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt
     3601 cagatcttct tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc
     3661 gcaactccag tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc
     3721 tttttttacg ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa
     3781 tttcgctttc gtgcactcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat
     3841 aaattatgag gtatatagtt gccttaacgg gaggcattgg cagtggcaag agtaccgttg
     3901 ccaatgcgtt tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg
     3961 tggttgaacc aggtgcacct gcgctacatg ccattgctga tcactttggc gctaacatga
     4021 ttgctgctga tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg
     4081 aagagaaaaa ctggcttaac gccctgctgc atccgctgat tcagcaagag acgcaacacc
     4141 agatccagca agctacttcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact
     4201 cactgtataa aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta
     4261 agcgcaccat gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc cttgctgctc
     4321 aggcaacgcg cgaagcccgc cttgccgtgg cagatgacgt cattgataat aacggcgcac
     4381 cggatgctat cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc
     4441 agtttgtctc acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa
     4501 tgaaaaaatg cgtacatggc tgcgcattga gtttttgatt cagcaactca ccgttaattt
     4561 acccatcgtt gaccacgctg gcgcgctgca tttcttccgt aatgtcagtg aattactgga
     4621 tgttttcgag cgcggcgaag tccgcactga gctgttgaaa gaacttgacc ggcagcaacg
     4681 taaactccag acctggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat
     4741 tcagcagtta aaagcggcgg ggagcgtatt aatttccgcg ccgcgtatcg ggcaatttct
     4801 gcgtgaagat cgtttgattg ctctggtgcg tcagcgactg agcatcccag gcggctgttg
     4861 cagctttgat ttacctacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag
     4921 ccaggtagaa acctggattg ccagcctgaa cccgctcacc caggcactta ccatggtgct
     4981 ggatttaatt cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca
     5041 ggataacggt ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt cacagcttta
     5101 tccgcaaatt tccggacata agagccgttt tgccattcgt tttatgccgc tggacagtga
     5161 aaacggacag gtaccggaac gtctagattt cgaactggcc tgttgctaag gagtgaaaat
     5221 gtcagaaact attacggtga attgcccaac ctgcgggaaa acggtggtat ggggtgaaat
     5281 cagcccgttt cggccatttt gctccaaacg ttgtcagctg atcgacctcg gagaatgggc
     5341 tgctgaagaa aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga
     5401 agagccaaag cagtgacatt ggtctgatgc cgcacatagg cctgataaga cgcggacagc
     5461 gtcgcatcag gcatcttgtg caaatgtcgg atgcggcgtg aacgccttat ccgacctaca
     5521 gactacagac gcttaagctt cgcaattacc ggttcattgg ctggcggaaa atcatcggca
     5581 ttaagaccga ccagcgacat ccactcaccg ggttgccctt ctttacccca cggcacccct
     5641 tcccaacttt cgaccagcca gaaccacaga gttatatgcc tgtccgggaa ttcatatgcc
     5701 agtttttcaa atagcgaaaa atgttgtggg gtaatcccga cctcttcctg aagttcacgc
     5761 accaccgcct gttccggcgt ttcgcccatt tcaattttac cgccgggaaa ctccagttta
     5821 ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt ctcgttgcga
     5881 ataataccta ccgcaatttg cagctttttc attgttttaa acctataaaa aaggcgcaga
     5941 atcctgcgcc ttttgcttca acagttagct tttattgcag gcggccatga cactgcttgt
     6001 attttttacc agaaccgcac gggcaaggat cgttacgtcc tactttgcgc tcaccggttt
     6061 gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg atggctaagc tgctgcattt
     6121 gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc tcttcaggca
     6181 tacgtacctg aactttgctc agcgtactga taacttcata tttcaacgac tccagcatcg
     6241 ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc tgtgcatagc
     6301 cacgcaggtg gataccctga cgcagatagt ccatcgctgc caggtgctct ttccacaggg
     6361 agtcaagcgt ttgcagcatg acgcctttct cgaagtgacg catcatctca gcaccaacca
     6421 cttcttcttt acgctgatac acttcgatgg actgcgccag aatgcgctca cgcagcgtct
     6481 cttcatgcag ttctggttct ttatccagcc actcggcaat tggtaaatcg aggtcgaaat
     6541 cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagc gactgtggcg
     6601 gaatgtaggc atcaatggtg gctttgaaca catcttcacg aatgctgttg atggtttcgc
     6661 tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga cgctgatcgt
     6721 tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacga ctttcaactt
     6781 tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct tcgcctggct
     6841 tcatacccag tttacgcatc atgccggata ctcggtcgga agcaaaaata cgcatcagcg
     6901 catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga ccagaacgac
     6961 cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgatg atatgcaggc
     7021 cacctgcttc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt ttttcaattt
     7081 gctctgcggt cggattttcc agcgcggcaa cttctgcctg ccagctacca ccgagcacaa
     7141 tatctgtacc acgacccgcc atattggtcg cgatagtcac cgcagccgga taacctgctt
     7201 gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacgttg tgcttaatac
     7261 cggctttggt cagttcgttt gacaccagct ccgatttttc gatggagata gtacccacca
     7321 gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc tgaatttttt
     7381 ccgcttcagt catgtagacc aggtccggca gatctttacg aatcattgga cggttggtcg
     7441 gaacaacgac ggtatccagc ttgtagatgg agctaaattc gaaagcttcg gtatcagcag
     7501 taccggtcat ccccgccagt ttttcataca gacggaagta gttctggaag gtgatcgaag
     7561 ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca gcctggtgca
     7621 gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca acgatgataa
     7681 cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga gcgcgcagcg
     7741 ccgccgttac gtggtgcatc agcatgatgt tggccggaga gtacagagac tccccttcat
     7801 ccatgatgcc ctctttcacc agcagttctt caatcagcac cagaccacgt tcggtcaggt
     7861 tcacctggcg agatttttcg tccaccgaga agtggccttc gccctggaag gtttcggagt
     7921 cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta tacatttccg
     7981 agctgtcttc tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg atcaggatgg
     8041 agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct tcagggctga
     8101 acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg taagtgatgt
     8161 cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg ataccgacag
     8221 tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc gccaggtagt
     8281 cgttgacggt aactacgtgc acgcctttac cggttagtgc gttcaggtaa gcaggcagcg
     8341 ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt tcgttaagaa
     8401 ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc ttacttgcct
     8461 cacgtaccac ggcgaaagct tccgggatca gattttccag cacttcgcct ttttccagac
     8521 gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt tccatctccg
     8581 gttccatggc attgatgatg ttgaccactt tgcgcatccg gcgcagggtg cgatcgttac
     8641 gactaccgaa aactttagtt aacaatttga ttagcataat aaaatctcaa acgccccgcg
     8701 ttgcggagtt aataaaatga agtaaaggtt tattgttgtt aggtgaggcg ttgagggcca
     8761 gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc ttgtggggta
     8821 aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg ggtcagcagc
     8881 gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga ttcttcagca
     8941 acgggcagtg tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt gcgaatggca
     9001 tattgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt cgcttccagc
     9061 aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt tgtcgctttt
     9121 gcgggcacgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact cgccgcgacc
     9181 atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca gcgcgtcagt
     9241 attccactca cgttattgcc atcccgatcg aattcaaacg tatttagcgc acaaatctta
     9301 tcattaaagc ctgaagtcca cagcatgaat gaagataagt tgtgcgtaaa agtgcttaaa
     9361 aaaacagcgt tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa acgactggtt
     9421 cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag tataaatgtc
     9481 gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg tcgtcctgga
     9541 aggtcacata ttcccaagct tcctgtttcg ccaggacagc ctgcagcagt ttgttattca
     9601 gtgcatgacc ggatttataa gcgataaatg caccaataat attgtgacca cacatgaaca
     9661 agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa cgcaggccgt
     9721 cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg ccgcccaggc
     9781 acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta cgcgcacggc
     9841 tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg ttgctggaat
     9901 caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg tacggcttaa
     9961 attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga acaaattttt
    10021 tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac ggagcggcgc
    10081 tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta tcgatgccca
    10141 ggcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca tgctcgttga
    10201 ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg aaatctaccg
    10261 gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca gggcgtaacg
    10321 tcagagtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg atacgtttaa
    10381 gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc gaagtgtact
    10441 atacattcgg cgggccagtt tagcacaaag agcctcgaaa ccaaaattcc agtcaattct
    10501 taatcagctt gcttacgcag gaatgctggg atatccagat aatccggctc tttcgcagtt
    10561 tgcggcgcat tgtcattcac gactttagca accggcttct gctcctgggt cagcggagcc
    10621 atcccatgct gctggtagcg atccatcact ggctgctgaa cctgcttatt ggtcaccaga
    10681 gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac gcgcagctcg
    10741 tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc ggaagcaaat
    10801 gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa gcccgccgtg
    10861 atgttaacca gcacgccgcg cgcgccagac aggtcgatat cttccagcag cggagaagag
    10921 atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc agaacccatc
    10981 attgcgtagc ccatctcaga cattacggtg cgtacgtctg caaagtccac gttcatcaaa
    11041 cccggacgag taatcagttc agcgatacct tgcacagcgc ctttcagtac atcgttcgct
    11101 gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag tttgtcgttc
    11161 gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg ctccgcgaat
    11221 gccatacgct tcttgccttc aaagttgaaa ggcttagtga cgacagcaac ggtcaggata
    11281 cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt accaccaccc
    11341 atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcaa tgcatcgcga
    11401 tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag tcctttggtg
    11461 ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc ttgtgcatcg
    11521 gtatttaccg cgaagaattc aacaccttca atgcgctcgc gcaccatgtg ttcaacagca
    11581 ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt aagttccatt
    11641 ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa tcatcgtcgg
    11701 cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat ccacgagcca
    11761 actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga ctctttccca
    11821 tagtgaagca atcccaccgc cgtcgaataa tacggctcct gagcataatc cgttaaaccg
    11881 gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg agcacaggct
    11941 gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc caggtgatgt
    12001 ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt gaccaggttg
    12061 agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg cagactccgt
    12121 ggcggacgac cgcctacgct cggcacttcc acgctctcat cttttccaac gatggaaccc
    12181 agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt gccaaaggcg
    12241 taagcgatat cactggtcac gacattgcca gcataaggaa ttaccttagt gtggcgcaat
    12301 gccccaccgg tataaacggc gatatccatt gtaccacctc cgatatcgac gacgcagaca
    12361 cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagtcc ggcaaatatc
    12421 agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt cgccatatcg
    12481 ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag tcctaccgga
    12541 ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg cagcacacga
    12601 tgttcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac atcttcttgc
    12661 gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct gatgtgctta
    12721 ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc tgcctggtca
    12781 atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc tttatccata
    12841 ccacgtgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg cagaacttcc
    12901 cctactaaag cggcaacctt cgcggtacca atctccagtc ctactaccag ttttctgtcc
    12961 gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta gattcctctg
    13021 gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca acgtagctaa
    13081 tccgtttgcc atcggtttgc gccagctgct gtaaaaccgg ataaagttct acaaagcgag
    13141 ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta ttcagcgtca
    13201 actgccagga acgccgcgcg gtcatcgccg tttccttcag agtaaatctg tcctttgcca
    13261 gcatctgccc catttcgcga tagccctgca acacttcatt ggcgctgcct tccgggccat
    13321 acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag gtgtttcctt
    13381 ccgcgtctac catatgttga tcattccacc gcgcaatcgg cacatattca accagatgaa
    13441 tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac ggcaggcgtt
    13501 gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc ggctcaccca
    13561 atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca ccggtcaaca
    13621 ccagctttga gagcggcagg cgttgcgcat cttccatcca gcccaacacg acccagccgc
    13681 tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga cgcgttccat
    13741 tattgcggcg agaagaaacc tcttcttcgc tgtttcgcgt gttcagagca gcctgcgaca
    13801 tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat acctgcctga
    13861 cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt ggcttccagc
    13921 agataaaact gtccatcgct gtccagcata acgtcaatac gtccccatcc tttgcaacct
    13981 aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga cgcatccaga
    14041 cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata atcatagaag
    14101 gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat cgcaaccgtg
    14161 aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg aaatgccaat
    14221 cttaatgcat cttgtagagc attttctgct actacttttg acattcccac actggaacct
    14281 tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc taactgctta
    14341 tcgctcaggc ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc gaccggtaaa
    14401 ccggcacctt gccacagcag tttgctgcgt agtttatcca ttgaaagcgc agatgccatc
    14461 actccgctgc cggtataagg caagcccatc agctcgagca tgccctgcag cgtaccatct
    14521 tcaccgccgc gaccgtgtag cgcgataaac actttctgaa aacccatcga cttcagttgc
    14581 gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa taccaccttc acgcagcccg
    14641 gctaacaccg ctgcgccaga attcagagaa acttcccgct cagcggaagt cccgcccaac
    14701 aggaccgcga ttttatcagt catgttgttc ttcctccgga gtttgcggct tcagtttgat
    14761 ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga gaatcaggtc
    14821 gttaccggtt aataccggtg ccagcatctc ggctacctgc gcagggtccg gcaccagaat
    14881 gggatcaatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt ccgctcccgg
    14941 aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa cctgcgtcag
    15001 cacattggcg aaatcatcat acaagtcgcg cgtacgggta aaacggtgcg gctgaaacag
    15061 cattaccagg tttttatccg gccagcctgc gcgcgccgct ttaatggtgg cgtcaacttc
    15121 cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac cattcactgg
    15181 ctccagcggg aattcaccga ggaaatcaaa acggcgacca gtccccagga agctctccag
    15241 cgcacgcaaa atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa ccgcagctgc
    15301 ggcgttcagc gcgttatgac ggcctggcgc attcagggtg acgcgcatcg gctctttgtc
    15361 ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt ctacacgcac
    15421 gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg gtaacaattc
    15481 gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaacggca ggttgtgcag
    15541 aaaattaata aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat ccatgtggtc
    15601 ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg atgcatcact
    15661 ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa cccccgccgc
    15721 ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt agatgctgga
    15781 aaccatcgcg gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc catgacgaaa
    15841 acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg cttcatgagc
    15901 tgcgacaatt tccgggttat cggcagaaat ggcgctggaa acaacgacca cgctggcatc
    15961 acgtacgttt tccgggcgat ggttgaaata aatcgtcgct cccagattca ttaactgctg
    16021 cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat tggccagaac
    16081 ttcggcaata ccgcccatac cggcaccacc gatgccgaca aaatgtatgt gccgaacgcg
    16141 acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca ttctttacgc
    16201 cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc ccgggcagcc
    16261 cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc gcgggcgcgt
    16321 tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc gacagcatcc
    16381 acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag cggtagcgca
    16441 ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg tagtcctgcc
    16501 gcggcgattt cactcaccgt taaagcaccg gagcggcaaa cgacgacatc cgcccacgca
    16561 tacgccgccg ccatatcatc aataaattcc gtcactttat gctgcggttg ccccgcttcg
    16621 gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca gatagtgact
    16681 gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg tgcgccctgg
    16741 gaaccaccca ctaccagcac acgaaccgga ccttcacgtc cagccaaacg ttgctgcggc
    16801 aacggcagcg ccagcacatc ggtacgcacc gggttaccca ccacttccgc attagggaaa
    16861 gcgcctggaa acgcctgcat cactttggtg gcaatcttcg ccagccattt attggttaag
    16921 cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca cgcggccaga
    16981 ccacctggac ctgatacata gccgcccata ccgagtacca cgtcaggttt gtacgctttc
    17041 ataatcgccc gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc tatcagtgct
    17101 tttatacctt ttccacgcag accagagata cgaatgaaat caatttcgat gccatgtttt
    17161 ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg ccaaccctga
    17221 gccattagat ggtgcgcaac cgccagtccc gggaatacat gtccaccggt tccgcctgcc
    17281 atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac gcctgcgctt
    17341 tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc gacataatca
    17401 gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag acctttggtc ggtaacatcc
    17461 ccgccgccgc gcctacgtta accagcgcct ggaagctaaa ccagatgcca atagaacagg
    17521 cgagaaaacc ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc gacatcgcgc
    17581 gaaaagcgac gaagaatacc attaaaagtg ccagcaccac accgacatac cccagttctt
    17641 cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc agtttttgta
    17701 ccgagttacc taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc agcgattgcg
    17761 ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg gtaacacggc
    17821 ggatacggta cggttcggcg agtatcagca acacaaccgc tgaaatgccc ataccgataa
    17881 tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc gtagtcacaa
    17941 acaccaccac caccgtacca aggtctggct gtgccagcag taacactgcc aacaccagaa
    18001 tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg cctttacgca
    18061 ccagatagtt ggcgatataa caaaacagcg acagttttgt cagctccgca ggctggatac
    18121 gcagcaaacc gagatcgatc caacgcgatg cccctttaac cgagctcccc actaccagca
    18181 cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt tgccagaact
    18241 ccatcggcag acgcagcgta atgatcgcca gaatgaatgc caggatcaga tagacgccat
    18301 cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc attgacgccg
    18361 aggtcaccat gataaagcca atcgccgcaa ggccgaaggt cagccacagt aaggtgcgat
    18421 cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag ccttttagcg
    18481 ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc aggcgaggga
    18541 gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc ctcgttgttc
    18601 aaagttcttg aactgatcaa ggctggcaca ggccggggag agcagaacca tatcgcccgg
    18661 ctgaacacgc ggagccagca agcgcatcgc ctgttccata gtttcggttt gttctgccac
    18721 ttccgggcgt agcgccgcca gctgcgcacc gtcacgacca aaacagtaca aacgcacgtt
    18781 gtcgccattc agatagcgcg ccagtggact gaagtccgcc gatttaccat cgccgcccag
    18841 caacaaatgc agcgtgccgt ctacctgcaa gccattcaac gccgcttccg tactgccgac
    18901 gttagtggct ttcgaatcgt taatccagcg tacgccattg tgctccagca ccacttcaaa
    18961 gcgatgcggc agaccggtga aggtggttaa cgctttcagg ctgctggcgc gcggcaaacc
    19021 agctgcgtct gccagtgcca gtgctgccag cgcattggtg tagttatgct gcccggaaag
    19081 tttcatctct ttcacgttca gcactttctc gcctttcact cgcagccagg tttctccctg
    19141 ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc gttcatcagc
    19201 accgcgaatc ggcattgtta aggcatcatc agcattgacg acacaaactt tcgcattttc
    19261 gtaaatacgc aattttgccg cacggtattg ctgcaaacca aacggatagc ggtccatatg
    19321 atcttcagtc acgttcagaa tggtcgccgc caccgcctgt aagctggaag tggtttccag
    19381 ctggaagctc gacagttcca gcacgtacag ttcgcactca gcatcaagca gcatcaacgc
    19441 tggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg ccatttcacc
    19501 cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa tcggtgcttg
    19561 tgcttcgcgg cagaacagct cgatatcgcc aacgatttcg attccggcat cagcggcagc
    19621 gcttaaggat ggatgcgcca gtgcaatacc gggactggcg acaatcagat ctgccgccat
    19681 cagccattca tcattcagac tgcccgtgtg gcgttctacg gcttcgggta atttatccag
    19741 gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag cgaggaaaaa
    19801 gtccacgcag gaaagcccgg tgaggcccag gccgataatg acgacatttt taccctgata
    19861 atcagccatg attaacgtac cttcagcgtt gccagaccaa tcagaaccag catcagcgaa
    19921 ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc atagtggtga
    19981 tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc gacctgcagg
    20041 atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag gaattcctga
    20101 cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc tacatcgccc
    20161 ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc cccgactatc
    20221 gcggtacaga caataaccag ttccccggcg tgtcgcagat acggtatgtg taaatagctg
    20281 gcaaagttca tgttgccggt cgcccacgcc accagcgcaa aaccaccggc gacaaatacg
    20341 gtcggcataa ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt gccagtaccc
    20401 acaatgacga agtaagccag cagaatgtag aacagcccca actgcggcat cacatcttta
    20461 aagaatggga ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta cagggcgaag
    20521 gcgacaccca gcgcaatgac cgacatccag aaatacttcc aacgagcgat caaccctttg
    20581 gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc gtaacctacc
    20641 agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag cagtacggag
    20701 atcacaatcg ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg cttgctgaag
    20761 tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg caaatgagca
    20821 atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct gacgatggcg
    20881 cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa atgttcggcc
    20941 agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc atggcggcac
    21001 tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt gatttaagac
    21061 gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg gtgctgatag
    21121 catggctttg tttacccacg cttaacacgc ggtcaatacc agcagctttt gccgcctcgc
    21181 ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg cccaccacca
    21241 gcacgcggta gcccggcatt tcagccagta cctgaacagc ggcggtcatt gaaccgacat
    21301 tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg gggaacagac
    21361 ggcctggaac agctttcaga tttgccagcc ccgctttgat agcatcaagc gttgcgccca
    21421 cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc cccggcaacg
    21481 gcagcagaac atcgacgcta ccggttgggg tttgtagggt aaattccgta ccgtgcgagg
    21541 tcacatggat attggtggcg gtgaaatcgc tgttggcggc attgggtgag aaacgccaca
    21601 ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg gcgttcataa
    21661 tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc gcgacacccg
    21721 caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc gcagcttccg
    21781 ggcgagtcag actcacagtc caggctattt cgccctgatg gttcgcgcca agttcaataa
    21841 ctgcgtaatc gtattccggc gttaagcgca acaacgtcat cggtacaccg atgtcgttgt
    21901 tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc gccgtcatct
    21961 ctttaacgga ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc ggaacttgct
    22021 ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg attaactgcg
    22081 gcaggtcgat atccagcgga cggctgacca gtagtgcgcc cgcgccgcca gctttcgcct
    22141 ggtcggcaaa atcgtgggca tcaaaacgtt cgcctttcag ggcaacaaac aggcagcccg
    22201 gcgtcagttt tcgggtatca gtggttacag catcaagggt gatatctgca ccttgcagtt
    22261 caccgttgag aatgtcggta agttggctaa gggttacgct aatcatgcaa tcacccccag
    22321 cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa caatctggta
    22381 atcttcatgg cctttgcccg cgaccagcac cacatcgttc tctttagcct gcataacggc
    22441 gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat ctaacattcc
    22501 cgccagaata tcgttgatga tggcacgcgg ttcttcggta cgcgggttat cgtccgtcac
    22561 caccgccacg tcagcaaact cttcggcaat tgcgcccatc agtggacgct tacctttatc
    22621 gcgatcgcca ccacagccaa agacacacca cagcttgccc gcacagtgca gacgcgccgc
    22681 ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca ccgtcggttt
    22741 gcctggcaca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg cggcggtttt
    22801 caacagatca gccaatggat agccgagtgc caacagtgtc gccagcgcga gcagcaggtt
    22861 gctgacgtta aaagcgccta tcagatggct ttcaatttcg ccatcgcccc aacttgagct
    22921 aaagcgaatc gtcacaccgc tgtcgtgata gttcacgtcg atcactttta accagcgtcc
    22981 gtgacagttc ggattaatat gatcttccat tgataccgca accgcgtccg gcagctttgc
    23041 cagccaacgg cggcccactt catcgtcggc gttaataatc gcctgaccgc aatgatgttc
    23101 agaataaagc agccatttcg cagcttcgta gtgttccata tcaccatgat aatcaaggtg
    23161 atcgcggctt aagttggtaa agaccgacgc cgcaaatttc aatgccgcca cacggtgctg
    23221 taccagcccg tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct ccaccagccc
    23281 cgccagctca tgctgaacat cgactgccga accggtggta ttttctgtcg ggatcacttt
    23341 ccccagcaga ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa gcagttggct
    23401 ccactgcgcc agcagctggg tagtcgtggt tttgccgttg gtgcccgtta cgcctacgag
    23461 acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata aacgctcgtt
    23521 gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac cgtcggtcgc
    23581 ctcatctttc gcctctgcaa taatggcagc cacaccttgc gctatcgcct gcgggatata
    23641 tcgacgcccg tccgcctgat gacctactac agctacaaag agatcgcccg ccgcagccac
    23701 acggctgtcg agtgtcatct ctcgcagtgc tcgcgaaggt gcgtctggca cccacggagc
    23761 aagaaggtcg cgcaaattac gatctgccac ctgtcccctc gccttgatta atcacaaatt
    23821 catttttatc gcccgttgtc agcgcatccg gctcgatgtt catggtacgc aatacgccgc
    23881 ccatgatggc accaaagacc ggcgcggaaa cggcgccgcc gtagtattta cccgcctgcg
    23941 gatcgttgat aacaacaacc agcgcgaagc gcggctgact cgcaggcgca acgcctgcgg
    24001 tataagcaat atatttattg atgtagcgac cgtccggccc gacctttttc gcggtaccgg
    24061 ttttaatggc gatacgatag cctttaatcg ccgccttcac gccgccgccg cccggtagcg
    24121 ccacgctttc catcatatgc accacggtgc ggacaatgga ttccgggaag acacgttcac
    24181 cgggaaccgg ggggtcaact ttggtaatcg acagtgggcg ataaatgccg tagctgccga
    24241 tagttgcgta gactcgcgct aactgtaacg gtgttaccat tagcccgtag ccgaaagaga
    24301 aggtggccct ctctatgtca gaccaccgtt gtttttgagg atataagcca ctgcgttctc
    24361 cgaccaaccc caaattggtc gcttttccca gtccaaaacg tgagtaagta tctactaacg
    24421 ctgaggacgg catcgctaac gccagcttgg aaacaccgac gttactcgac ttctgtaata
    24481 ccccggtcag ggttaattcg ctgtagcgtg ccacgtcttt gatttcgtgg ccgttaattc
    24541 gataaggaat ggtattgagt accgagtttt cccgcaccac gccacgttgc aacgcggtca
    24601 tcaccaccat cggtttaacc gttgagcccg gttcaaacac gtcggtgatg gtacggttac
    24661 gcatcgcctc tttcggcgtg ccgctcagat tgttagggtt gtatgacggg ctgttagcca
    24721 tcgccagcac ttcacctgtg ttgacatcca ccagcacggc gctaccagat tcagccttgt
    24781 taaaggccac cgcgttgttc agttcgcgat aaaccagcgc ctgcaggcgt tcatcaatac
    24841 tcagcgccag gttgtgcgct gcctggctgt cagtagaaga aatatcttca attacgcgac
    24901 catagcggtc tttacgcaca atgcgctcac ccggctgccc ggtaagccat ttatcgaaac
    24961 tcttctcaac gccctcaatc ccttgactat cgacgttagt aaagccaatg aggtgagcag
    25021 tcacttcgcc ggatggataa taacggcgag attcttcacg cagatgaata cccggcagtt
    25081 tcagtttttt gatgtagtcc gccatgtcag ggtttacctg acgcgccaga taaataaagc
    25141 gccctttcgg gttggcgtta atgcgggcag aaagctgatc cagcggaata ttgagcgcgt
    25201 tagccagcgc cttccagcgg tcaccgacgc tgataccgcc agcgtcatgc acttctttcg
    25261 ggtcagccca aatcgctttt accggcacgc tcaccgctaa cgggcgacca gaacggtcag
    25321 taatcatgcc gcgggaggtg gaaacttgct gaacgcgaag agaacgcatg tcgccctctt
    25381 tcaccagcat atccggggag ataacttgta accacgctac gcgtccgagc agaaaagcca
    25441 gcgccaggag aatacagccg cataacaacg caaaacgcca actaataaag ttggcatgtt
    25501 cttcctgacg ttttggtttc tgcgttttcg ccgctgcttt catgcgtcgc gtttatcctt
    25561 atttttgcac tacgatattt tcttgtgacg gatcaacatg ctgcatttgc agcttttccg
    25621 tggcgatcct ttccacccgg ctatggtcgc cgagcgcatt ctcttcaagg atcaggttgc
    25681 gccattcaat gtctaaagca tctcgctcca gcaccagttg ttcgcgctga gcggtcagta
    25741 aacgggtatg gtgcgccgtg gttaccacgg tcaccgccgt caaaataatg caaatgaaca
    25801 ggcagagtgg cagcttccca aatcgcaaaa gatcgtcacc gataacacca ggcaatgcat
    25861 ggcgctcgtg gcttcccatc gatcctttaa ctttgcttag agcttctgtc actctgctga
    25921 tcatgcgttc gtcctctctg cgatacgcag aactgaacta cgggcacgag gattctcagc
    25981 cacctcttct tcgcccggca ttaacttgcc taatgctcgc agctgacggc cacccagttt
    26041 tttgagctgc tcttcagtca tcggtaaccc tgccggaact tgcggaccgc ggctgttttc
    26101 acgcataaaa cgtttcacaa tacggtcttc cagcgagtgg aagctgatga tcgaaagccg
    26161 cccacccggg gccagcacgt tgagcgagct ttttagcgcc tgctctatct cctccagttc
    26221 actgtttacc caaatgcgca ccgcctggaa ggtacgggtc gcgggatgtt taaacttatc
    26281 tttcaccggc gttgcagcag ccacgacttc cgccagttct ttggtgcggg tcatcggctg
    26341 ttcgcggtta cgctcgacaa tggcgcgggc aatgcgtttg gcaaaacgct cttcaccata
    26401 ggttttcaat acccaggcga tatcggcttc ttctgcggtt tgtagccatt cagcggctga
    26461 ctgcccacgg gttgggtcca tacgcatgtc cagcggacca tcgcgcataa aggaaaagcc
    26521 acgttcagca tcatcaagtt gcggtgaaga gacgccaaga tcgaggagaa tgccgtcgat
    26581 cttgccgata agatcgcgct cggcaacgta ttcgcccagc gcggagaaag gtccgtggat
    26641 gatggagaag cgcggatcat caatagtctt cgcaacggcg atagcctgcg ggtcgcgatc
    26701 gatcgccagc aaacgcccct cttcgccaag ctgcgagagg atcagacgtg agtgaccacc
    26761 gcgaccaaaa gtcccatcaa tgtagatgcc atcaggacgg atattgaggc cattaacggc
    26821 ttcatccagc agcaccgtag tatgtttata gttttccatc attttataga gacaagtcct
    26881 gcagtcgctc cgataagtct ccagtagcca actgctctgc gtcgatatct tccttgacct
    26941 gttgatgcca ggttgtttca tcccacagct caaacttgtt gaactgtcca accagcatca
    27001 cttcttttgt cagcccggca tgttgccgca gtactggcgc gattaacaat cgacctgcgc
    27061 catccatctg acattcgctg gcatgaccta acagtaggcg ctgcacacgg cgctcaactg
    27121 ggttcatgct cgacagacgc gataattttt gctcgataat ttcccattca ggcagggggt
    27181 aaagcagcag gcacgggtga tgaatgtcaa tggtgcaaac catttgaccg gcagcgttct
    27241 caagcagctg ttcccgataa cgggtaggca ctgataagcg ccctttgctg tcgagattga
    27301 ctaacgttgc tccccggaac atgccagcct caccccttat tcccactttg ccccacaatt
    27361 tcccactgaa aggagtttac ggagcggagg aaaagcttgt caagccagtc acaaggcata
    27421 ccgaggtaaa aaacccgatg tttacggcta atatcagcgt tgtttatttt acgtccaacg
    27481 cgcgaagcaa attaacgtta tgaatagctg taagaaaaaa tccctcatcg tcatcactgt
    27541 tttaaaatga ctcactattt tccacagcaa atataaagtg tcagtttgcg acgcgagcgg
    27601 cattttagga catatcttcc ccggttaaca gtccttgtta cgtctgtgtg gcgcttgatg
    27661 ccagcgggtt tgcgcaaaag caaagaaaag tgtttgttaa atcggcaatt catcgccgca
    27721 tgtaacaaaa taatgcaaaa acgcgctgtc gatatcactt aattattgtg aatttcagct
    27781 ggtgagttaa ttaacgggcg ggtttaaaac catttatcaa taatagtcag gaatagtctt
    27841 atttacttta agcatattga tgtccagtcc cgtactctac gcgccagagg gaaattcacc
    27901 tggcgcgtat ttttgttcgc ggcttagcta cggctgagca caccgcggcg atagagatta
    27961 cgtttaatgc gcgttaaacc aggttttggc ttacgcggtt cgtccaggct tgccaggaca
    28021 atctccagca cacgctctgc gacatcgcgg tgacgttgag ccactgccag caccgggcac
    28081 tgtaagaagt cgagcagttc gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt
    28141 ttaccatcgc gacgtagcgt gacatccatc accccttgca acaacgcaaa cgacgttgtg
    28201 aacagcgcct gcggcatcag atgcgtttcc agccattttt cgaataactg ggcagccgcc
    28261 tcccgctcat agctgttggc atacaggaaa tgcacttcgc gcggatcatc tttccaggca
    28321 gtacggaaac cttgttcacg caggaagctg acagaaagct ccggcagcgc gccaagataa
    28381 agcaccgtct cggcgggaaa cttacgtaac tcttccgcca gcatttcggc atcatcctga
    28441 tcggcaccaa ccacgctggt gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc
    28501 gggaacgggg cgttagccca gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa
    28561 acaataatgg catcaacctg acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct
    28621 ggctgatctt ctgagcaggc aatcagcagt tgataacccc gttgccgcgc ctggcgctca
    28681 agatagttag cgatgcgggt atagctggtg ttctccagat cggggatcac aagaccaata
    28741 gaacgtgtgc gtccagcacg aagcccagct gccacggcgt tcgggtggta attgtgctca
    28801 cgcaccacag ccatgacttt ttcaacggtt ttgtcgctca cacggtattg cttcgctttg
    28861 ccgttaataa catagcttgc agtggtccgc gacactcccg ccagccgagc gatttcatcc
    28921 agtttcacaa ttgccccttg cgtaaaatgt aaaaaccata gccattgtac tggtatgggt
    28981 taaattcatt aacatctaag cgcagaaaat taactgcggc aactgctttt attccaggtt
    29041 cccactgatt tcgcaaaaaa agcccagcgt gacatgttgg gctgcaaatt gcacactgag
    29101 atcattctca acgcattatt ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa
    29161 cttccacaat tttcgccacc tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg
    29221 tgcctgctaa ttgaacggta taaagcgagg gggtgacatc gataatttgc ccacggaata
    29281 tttccgtatt acgtttcact tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca
    29341 tgatttcccg ctcaacatgc gcgccctgcc ccaactcact cacgcgcaag acatcgacca
    29401 gcttgtgtaa ttgcttttcg atctgctcaa gtactttttc atcgcccacg gtctggatgg
    29461 tcatacgcga taatgtcgga tcgtcggttg gcgcaacggt caggctttca atgttgtagc
    29521 ctcgctggga aaaaaggcca atcacgcggg ataacgcgcc tgattcattt tcgagtaaga
    29581 ctgataatat ccggcgcata atcaggttct ctccgttttg cttaaccaca tttcatccat
    29641 tccgcccccg cgaatctgca tcgggtagac gtgctcactg ccatcgacgg taacatcaac
    29701 aaacaccagg cgattattgc gcacctgttc cagcgcctcg ctaagtttgc tttccagctc
    29761 atgcggatga gagatctgga tcccgacgtg accataggct tccgccagac ggacgaaatc
    29821 gggtagtgat tgcatataag attgtgaatg acggccggaa tagatcatgt cctgccactg
    29881 tttcaccatc cccagatagc ggttattgag attcaccacc agtacgggca actcgtattg
    29941 caacgcggta gacagttcct gaatgttcat ctgaatactg ccgtcgccag tgacgcaaac
    30001 cacggtttct tctggcaacg ccattttgac gcccagtgcc gcaggtaaac caaaacccat
    30061 cgtgccgagg ccaccggaat tgatccagcg acgcggtttg tcgaatggat aataaagtgc
    30121 agcaaacatc tggtgctgcc cgacatcgga cgtcacgtaa gcgtctccct tcgtcaaccg
    30181 ccaaagagtc tcgatcaccg cctgcggttt aatcttttca ctgtgagtgt catatttcag
    30241 gcactgacga gcgcgccact gttcaatttg ctgccaccag tcgcggatct catccagtgg
    30301 ttgatgggcg gattcttgcg acaagagttc aagcatttgt tcgaggacct ggcgagcatc
    30361 ccccacaatc gggatatccg cagtcacggt tttagaaatg gaaataggat caatatcgat
    30421 atgcagaaca gtggcatttg ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa
    30481 tcgtaccccg acggcgaaaa tcacatccgc gttatgcatc gtcatattgg cttcgtaggt
    30541 accgtgcatt cccagcatgc ccagtgcctg acgatgcgtt gccggaaacg cccccagccc
    30601 catcaatgag gaaacaacgg gcagattcaa cgcctccacc gtttctttca actgctgatg
    30661 gcagcccgcc atgattgccc caccgcctac gtagacaacc ggtttttttg ccactaccag
    30721 cgtttgcaga gcacgcttaa tttgcccttt atgtccggta gtagtgggat tgtaagaacg
    30781 catactgacc gactccggcc agacataggg taatttgttc gccggattaa gaatatcttt
    30841 cggtaaatca acgactactg gtccaggacg accacttgcc gccagccaga aagccttttt
    30901 cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa ctgtgtttaa ccactggtcg
    30961 cgaaatcccc accatgtcgc actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac
    31021 ctgcccggaa aggacaacta atggaatgga atccatataa gcggtggcga tgccggtaat
    31081 cgcattggtc gcccctggac ccgacgttac cagcacgacg ccgacttccc cggtcgcgcg
    31141 cgccagacca tcggccatat gcactgccgc ctgctcatga cgaactaaaa catgatcgat
    31201 accacccacg gtatgcaatg catcgtaaat atcaaggact gcgcctccag gataaccgaa
    31261 tacttgttta acgccctgat cgataagcga tcggacgacc atctcggctc cagacaacat
    31321 ctccatggcc tgcctcactg tttgatggaa aaaatgtgta aaacacatta accgtccgat
    31381 cttgcttaag caattggcag ccagagacat aaacgcaggg taataagaat aaacaggagg
    31441 aaagatgaaa agaaacgata aaatcctcca ttacgaagga ttaaattgat aataaatcgg
    31501 ctgaatccca caacttacag caatttctct atttttttaa ttaatcctaa aatagagagc
    31561 ccacacattc agaataaaat tctacattca ataaaaaatg cagaataaac cagacattca
    31621 tgtctgacct attctgcatt cagttagcgt ttgcaaattg agactaattg ttcttccatc
    31681 cactgatggc ctttatcgcg cccggcagct tcatgccatg agagataaca ggttctgctg
    31741 ttttgtttta acggcagcgg taatacctgt aattctaagg attcagcgaa ctcttcagcc
    31801 agccaacgcg gcgcaatagc gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc
    31861 attgccatgc cctgatacgc gatactggct tgcttatcta ccgtgtcata ccaaggttga
    31921 ctaaatgacg cgaaacgatc gagcgaaacc gccgcatgtt gttcgttata aacatcatgt
    31981 ttcagtaacg ggcctttaat tgttggatga tttttgctgg ctaccagcac catttcatct
    32041 ttaaataatg gcacgctggt aaattcagga cgatggaagt cttcataact aatcacaaac
    32101 tccgtttcct gataacgcag ctgatgttca gtgttctgat ttaatgaaga cttgaacata
    32161 acatgtatat ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt cagaatgctg
    32221 tctaacgggc tgcaaacaca aagatgaaat acacgttcac tactcgcggg ttcaaaacct
    32281 gaaccaggca attcattttg tactagttgc aatgcctggc gaactgaacc aaaaagttga
    32341 aatgcgcgag cagtcggttg aataccacgg ccataacgaa caaaaagctc gtcattaaac
    32401 atcaccttca ggcgtgcaac agcgttactg accgcaggtt gcgacatacc cagaacatga
    32461 gcggcacggg taatgttttg ctcctgcatc acggcatcga aaacggttaa taagttgaga
    32521 tcgaccatgc gtagctgtgg cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc
    32581 tctggcatac ttaactccac tgtcacactt aactcccttt cccttattgg aatgcagaga
    32641 aataatcctg aagaat
//
LOCUS       DAJTNN010000026        31106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTNN010000026 DAJTNN010000000
VERSION     DAJTNN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.026"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO150_001092"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO150_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5515314.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO150_001093"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO150_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5515315.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1338..1766
                     /gene="rplK"
                     /locus_tag="OO150_001094"
     CDS             1338..1766
                     /gene="rplK"
                     /locus_tag="OO150_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5515316.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGAGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1770..2474
                     /gene="rplA"
                     /locus_tag="OO150_001095"
     CDS             1770..2474
                     /gene="rplA"
                     /locus_tag="OO150_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5515317.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2887..3384
                     /gene="rplJ"
                     /locus_tag="OO150_001096"
     CDS             2887..3384
                     /gene="rplJ"
                     /locus_tag="OO150_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5515318.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3451..3816
                     /gene="rplL"
                     /locus_tag="OO150_001097"
     CDS             3451..3816
                     /gene="rplL"
                     /locus_tag="OO150_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5515319.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4136..8164
                     /gene="rpoB"
                     /locus_tag="OO150_001098"
     CDS             4136..8164
                     /gene="rpoB"
                     /locus_tag="OO150_001098"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5515320.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8241..12464
                     /gene="rpoC"
                     /locus_tag="OO150_001099"
     CDS             8241..12464
                     /gene="rpoC"
                     /locus_tag="OO150_001099"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5515321.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12677..13216
                     /locus_tag="OO150_001100"
     CDS             12677..13216
                     /locus_tag="OO150_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HCR5515322.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OO150_001101"
     CDS             complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OO150_001101"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5515323.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAKTEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OO150_001102"
     CDS             complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OO150_001102"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5515324.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OO150_001103"
     CDS             complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OO150_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5515325.1"
                     /translation="MQILFNDQPMQCATGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OO150_001104"
                     /pseudo
     CDS             complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OO150_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OO150_001105"
     CDS             complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OO150_001105"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5515326.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVISAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OO150_001106"
     CDS             complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OO150_001106"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5515327.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19071..19171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OO150_001107"
     CDS             complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OO150_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5515328.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQKVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19795..20568
                     /gene="nudC"
                     /locus_tag="OO150_001108"
     CDS             19795..20568
                     /gene="nudC"
                     /locus_tag="OO150_001108"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5515329.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20608..21672
                     /gene="hemE"
                     /locus_tag="OO150_001109"
     CDS             20608..21672
                     /gene="hemE"
                     /locus_tag="OO150_001109"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5515330.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKVTRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21682..22353
                     /gene="nfi"
                     /locus_tag="OO150_001110"
     CDS             21682..22353
                     /gene="nfi"
                     /locus_tag="OO150_001110"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5515331.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22396..22986
                     /locus_tag="OO150_001111"
     CDS             22396..22986
                     /locus_tag="OO150_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5515332.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVVLSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23173..23445
                     /gene="hupA"
                     /locus_tag="OO150_001112"
     CDS             23173..23445
                     /gene="hupA"
                     /locus_tag="OO150_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5515333.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23458..24153
                     /locus_tag="OO150_001113"
     CDS             23458..24153
                     /locus_tag="OO150_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5515334.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OO150_001114"
     CDS             complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OO150_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5515335.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24818..26194
                     /gene="zraS"
                     /locus_tag="OO150_001115"
     CDS             24818..26194
                     /gene="zraS"
                     /locus_tag="OO150_001115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5515336.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26191..27515
                     /gene="zraR"
                     /locus_tag="OO150_001116"
                     /pseudo
     CDS             26191..27515
                     /gene="zraR"
                     /locus_tag="OO150_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OO150_001117"
     CDS             complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OO150_001117"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5515337.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLLLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OO150_001118"
     CDS             complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OO150_001118"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5515338.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            31018..>31106
                     /locus_tag="OO150_001119"
     rRNA            31018..>31106
                     /locus_tag="OO150_001119"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7391 a         8018 c         8468 g         7229 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgactcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcttggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgagtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt atttttatgg
     1261 gccttgcccg taaaaacgat tttttatatc acggggagcc tctcagaggc gttattaccc
     1321 aacttgagga atttataatg gctaagaaag tacaagccta tgtcaagctg caggttgcag
     1381 ctggtatggc taacccgagt ccgccagtag gtccggctct gggtcagcag ggcgtaaaca
     1441 tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga
     1501 ttccggtagt aatcaccgtt tacgctgacc gttctttcac tttcgttacc aagaccccgc
     1561 cggcagcagt tctgctgaaa aaagcggctg gtatcaagtc tggtgccggt aagccgaaca
     1621 aagacaaagt gggtaaaatt tcccgcgctc agctgcagga aatcgcgcag accaaagctg
     1681 ccgacatgac tggtgccgac attgaagcga tgactcgctc catcgaaggt actgcacgtt
     1741 ccatgggcct ggtagtggag gactaagaaa tggctaaact gaccaagcgc atgcgtgtta
     1801 tccgcgagaa agttgatgca accaaacagt acgacatcaa cgaagctatc gcactgctga
     1861 aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg
     1921 acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt actgccgcac ggtactggcc
     1981 gttccgttcg cgtagccgta tttacccaag gtgcaaacgc tgaagctgct aaagctgcag
     2041 gcgcagaact ggtaggtatg gaagatctgg ctgaccagat caagaaaggc gaaatgaact
     2101 ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc
     2161 tgggtccgcg cggcctgatg ccaaacccga aagtgggtac tgtaacaccg aacgttgctg
     2221 aagcggttaa aaacgctaaa gctggccagg ttcgttaccg taacgacaaa aacggcatca
     2281 tccacaccac catcggtaaa gtggactttg acgctgacaa actgaaagaa aacctggaag
     2341 ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga
     2401 aagttagcat ctccaccacc atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg
     2461 cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt ctacaatctt acccccacgt
     2521 ataatgctta atgcagacgt atatccgaga tattcgggtt gtggcaaggc ggcaactgag
     2581 tgagtcgcca agagcatagc taactatgtg actggtgcga atgaaggaag ccaacgccgc
     2641 cacaagttga atagcgacgg atagaaaaga tttgttcgtt ggagcttggc ctatccaggc
     2701 ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc ccctgcgtag acggtgacag
     2761 aacgctaaga ttattctttt atattctggc ttgtttctgc tcaccgtaat taagacgctc
     2821 tctccgtttg gaggagtgaa gtgagttcca gagattttct ctggcaaaca tccaggagca
     2881 aagctaatgg ctttaaatct tcaagacaaa caagcgattg ttgctgaagt cagcgaagta
     2941 gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg gcgtaactgt agataaaatg
     3001 actgaactgc gtaaagcagg tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc
     3061 ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt
     3121 ccgaccctga ttgcatactc tatggaacac ccgggcgctg ctgctcgtct gttcaaagag
     3181 ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg cctttgaagg tgagctgatc
     3241 ccggcgtctc agatcgaccg cctggcaact ctgccgacct acgaagaagc aattgcacgc
     3301 ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg ttcgtactct ggctgctgta
     3361 cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt aacgcattcg cttacgtata
     3421 aacttattct gatattcagg aacaatttaa atgtctatca ctaaagatca aatcattgaa
     3481 gcagttgcag ctatgtctgt aatggacgtt gtagaactga tctctgcaat ggaagaaaaa
     3541 ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg gcccggttga agctgctgaa
     3601 gaaaaaactg aattcgacgt aattctgaaa gctgctggcg ctaacaaagt tgctgttatc
     3661 aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag ctaaagacct ggtagaatct
     3721 gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg cagaagcact gaaaaaagct
     3781 ctggaagaag ctggcgctga agttgaagtt aaataagcca acccttccgg ttgcagcctg
     3841 agaaatcagg ctgatggctg gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc
     3901 cagtagcgtt tcacactgtt tgactactgc tgtgcctttc aatgcttgtt tctatcgacg
     3961 acttaatata ctgcgacagg acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg
     4021 tgatagcaac aggcattgcg gaaagtgttc cattttccgg tcaacaaaat agtgttgcac
     4081 aaactgtccg ctcaatggac agatgggtcg acttgtcagc gagctgagga accctatggt
     4141 ttactcctat accgagaaaa aacgtattcg taaggatttt ggtaaacgtc cacaagttct
     4201 ggatgtacct tatctccttt ctatccagct tgactcgttt cagaaattta tcgagcaaga
     4261 tcctgaaggg cagtatggtc tggaagcagc tttccgttcc gtattcccga ttcagagcta
     4321 cagcggtaat tccgagctgc aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt
     4381 ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct
     4441 ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa gacattaaag aacaagaagt
     4501 ctacatgggc gaaattccgc tcatgacaga caacggtacc tttgttatca acggtactga
     4561 gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg
     4621 taaaacccac tcttcgggta aagtgctgta taacgcgcgc atcatccctt accgtggttc
     4681 ctggctggac ttcgaattcg atccgaagga caacctgttc gtacgtatcg accgtcgccg
     4741 taaactgcct gcgaccatca ttctgcgcgc cctgaactac accacagagc agatcctcga
     4801 cctgttcttt gaaaaagtta tctttgaaat ccgtgataac aagctgcaga tggaactggt
     4861 gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta
     4921 cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc cagctggaaa aagacgacgt
     4981 caaacttatc gaagtcccgg ttgagtacat cgcaggtaaa gtggttgcta aagactatat
     5041 tgatgagtct accggcgagc tgatctgcgc agcgaacatg gagctgagcc tggatctgct
     5101 ggctaagctg agccagtctg gtcacaagcg tatcgaaacg ctgttcacca acgatctgga
     5161 tcacggccca tatatctctg aaaccttacg tgtcgaccca actaacgacc gtctgagcgc
     5221 actggtagaa atctaccgca tgatgcgccc tggcgagccg ccgactcgtg aagcagctga
     5281 aagcctgttc gagaacctgt tcttctccga agaccgttat gacttgtctg cggttggtcg
     5341 tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa ggttccggta tcctgagcaa
     5401 agacgacatc attgatgtta tgaaaaagct catcgatatc cgtaacggta aaggcgaagt
     5461 cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa
     5521 ccagttccgc gttggcttgg tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg
     5581 cgatctggat accctgatgc ctcaggatat gatcaacgcc aagccgattt ccgcagcagt
     5641 gaaagagttc ttcggttcca gccagctgtc tcagtttatg gaccagaaca acccgctgtc
     5701 tgagattacg cacaaacgtc gtatctccgc actcggccca ggcggtctga cccgtgaacg
     5761 tgcaggcttc gaagttcgag acgtacaccc gactcactac ggtcgcgtat gtccaatcga
     5821 aacccctgaa ggtccgaaca tcggtctgat caactctctg tccgtgtacg cacagactaa
     5881 cgaatacggc ttccttgaga ctccgtatcg taaagtgacc gacggtgttg taactgacga
     5941 aattcactac ctgtctgcta tcgaagaagg caactacgtt atcgcccagg cgaactccaa
     6001 cctggatgaa gaaggccact tcgtagaaga cctggtaact tgccgtagca aaggcgaatc
     6061 cagcttgttc agccgcgacc aggttgacta catggacgta tccacccagc aggtggtatc
     6121 cgtcggtgcg tccctgatcc cgttcctgga acacgatgac gccaaccgtg cattgatggg
     6181 tgcgaacatg caacgtcagg ccgttccgac tctgcgcgct gataagccgc tggttggtac
     6241 tggtatggaa cgtgctgttg ccgttgactc cggtgtaact gcggtagcta aacgtggtgg
     6301 tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa gttaacgaag acgagatgta
     6361 tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa tacacccgtt ctaaccagaa
     6421 cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt
     6481 gctggcagac ggtccgtcca ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt
     6541 agcgttcatg ccgtggaatg gttacaactt cgaagactcc atcctcgtat ccgagcgtgt
     6601 tgttcaggaa gaccgtttca ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga
     6661 caccaagctg gggccggaag agatcaccgc tgacatcccg aacgtgggtg aagctgcgct
     6721 ctccaaacta gatgaatccg gtatcgttta cattggtgcg gaagtgaccg gtggcgacat
     6781 tctggttggt aaggtaacgc cgaaaggtga aactcagctg accccagaag aaaaactgct
     6841 gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac tcttctctgc gcgtaccaaa
     6901 cggtgtatcc ggtacggtta tcgacgttca ggtctttact cgcgatggcg tagaaaaaga
     6961 caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag gcgaagaaag acctgtctga
     7021 agaactgcag atcctcgaag cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg
     7081 tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat cgctggctgg agctgggcct
     7141 gacagacgaa gagaaacaaa atcagctgga acagctggct gagcagtatg acgaactgaa
     7201 acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct
     7261 ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg gttaaacgcc gtatccagcc
     7321 tggtgacaag atggcaggtc gtcacggtaa caagggtgta atttctaaga tcaacccgat
     7381 cgaagatatg ccttacgatg aaaacggtac gccggtagac atcgtactga acccgctggg
     7441 cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc cacctgggta tggctgcgaa
     7501 aggtatcggc gacaagatca acgccatgct gaaacagcag caagaagtcg cgaaactgcg
     7561 cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt cgtcagaaag ttgacctgag
     7621 taccttcagc gatgaagaag ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat
     7681 cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg
     7741 cgacctgccg acttccggtc agatccgcct gtacgatggt cgcactggtg aacagttcga
     7801 gcgtccggta accgttggtt acatgtacat gctgaaactg aaccacctgg tcgacgacaa
     7861 gatgcacgcg cgttccaccg gttcttacag cctggttact cagcagccgc tgggtggtaa
     7921 ggcacagttc ggtggtcagc gtttcgggga gatggaagtg tgggcgctgg aagcatacgg
     7981 cgcagcatac accctgcagg aaatgctcac cgttaagtct gatgacgtga acggtcgtac
     8041 caagatgtat aaaaacatcg tggacggcaa ccatcagatg gagccgggca tgccagaatc
     8101 cttcaacgta ttgttgaaag agattcgttc gctgggtatc aacatcgaac tggaagacga
     8161 gtaattctcg ctcaaacagg tcactgctgt cgggttaaaa cccggcagcg gattgtgcta
     8221 actccgacgg gagcaaatcc gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa
     8281 ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc agacatgatc cgttcatggt
     8341 ctttcggtga agttaaaaag ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg
     8401 acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta
     8461 agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc
     8521 agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg actgcgcaca
     8581 tctggttcct gaaatcgctg ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg
     8641 atatcgaacg cgtactgtac tttgaatcct atgtggttat cgaaggcggt atgaccaacc
     8701 tggaacgtca gcagatcctg actgaagagc agtatctgga cgcgctggaa gagttcggtg
     8761 acgaattcga cgcgaagatg ggtgcggaag caatccaggc tctgctgaag agcatggatc
     8821 tggagcaaga gtgcgaacag ctgcgcgaag agctgaacga aaccaactcc gaaaccaagc
     8881 gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac
     8941 cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt ccgctggttc
     9001 cgctggatgg tggtcgtttc gcgacttctg acctgaacga tctgtatcgt cgcgtcatta
     9061 accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc gccggacatc atcgtacgta
     9121 acgaaaaacg tatgctgcag gaagcggtag acgccctgct ggataacggt cgtcgcggtc
     9181 gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta
     9241 aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg
     9301 taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc
     9361 tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt gctaccacca
     9421 ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat atcctggacg
     9481 aagttatccg cgaacacccg gtactgctga accgtgcacc gactctgcac cgtctgggta
     9541 tccaggcatt tgaaccggta ctgatcgaag gtaaagctat ccagctgcac ccgctggttt
     9601 gtgcggcata taacgccgac ttcgatggtg accagatggc tgttcacgta ccgctgacgc
     9661 tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc ctgtccccgg
     9721 cgaacggcga accaatcatc gttccgtctc aggacgttgt tctgggtctg tactatatga
     9781 cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg aaagaagcag
     9841 aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg
     9901 agtatgaaaa agatgcaaac ggtgaattag tagcgaaaac cagcctgaaa gacacgactg
     9961 ttggccgtgc cattctgtgg atgattgtac cgaaaggtct gccttactcc atcgtcaacc
    10021 aggcgctggg taaaaaagca atctccaaaa tgctgaacac ctgctaccgc attctcggtc
    10081 tgaaaccgac cgttattttt gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc
    10141 gttctggtgc atctgttggc atcgatgaca tggtcatccc ggagaagaaa cacgaaatca
    10201 tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct ggtctggtaa
    10261 ctgcgggcga acgctacaac aaagttatcg atatctgggc tgcggcgaac gatcgtgtat
    10321 ccaaagcgat gatggataac ctgcaaactg aaaccgtgat taaccgtgac ggtcaggaag
    10381 agaagcaggt ttccttcaac agcatctaca tgatggccga ctccggtgcg cgtggttctg
    10441 cggcacagat tcgtcagctt gctggtatgc gtggtctgat ggcgaagccg gatggctcca
    10501 tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc cagtacttca
    10561 tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaact gcaaactccg
    10621 gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc gaagacgatt
    10681 gtggtaccca tgaaggtatc atgatgactc cggttatcga aggtggtgat gttaaagagc
    10741 cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg
    10801 ctgatatcct cgttccgcgc aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag
    10861 agaactctgt cgacgcggtt aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg
    10921 tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac aagggtgaag
    10981 caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg accatgcgta
    11041 cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc caggtgaaaa
    11101 acaaaggtag catcaagctc agcaacgtga agtcggttgt gaactccagc ggtaaactgg
    11161 ttatcacttc ccgtaatact gaactgaaac tgatcgacga attcggtcgt accaaagaaa
    11221 gctacaaagt accttacggt gcggtactgg cgaaaggcga tggcgaacag gttgctggcg
    11281 gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt
    11341 ttgtacgctt tactgacatg atcgacggtc agaccattac tcgtcagaca gacgaattga
    11401 ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt ggtaaagatc
    11461 tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatt ccgggtaccg
    11521 atatgcctgc gcagtacttc ctgccgggta aagcgattgt tcagctggaa gatggcgtac
    11581 agatcagctc tggtgacacc ctggcgcgta ttccgcagga atccggcggt accaaggaca
    11641 tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc acgtcgtccg aaagagccgg
    11701 caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc
    11761 gtcttgttat caccccggta gacggtagcg atccgtacga agagatgatt ccgaaatggc
    11821 gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc
    11881 cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgttact cgttacatcg
    11941 ttaacgaagt acaggacgta taccgtctgc agggcgttaa gattaacgat aaacacatcg
    12001 aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt taacgcgggc agctccgact
    12061 tcctggaagg cgaacaggtt gaatactctc gcgtcaagat cgcaaaccgc gaactggaag
    12121 cgaacggcaa agtgggtgca acttactccc gcgatctgct gggtatcacc aaagcgtctc
    12181 tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc gtgctgaccg
    12241 aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg
    12301 gtcgtctgat cccggcaggt accggttacg cgtaccacca ggatcgtatg cgtcgccgtg
    12361 ctgcgggtga agctccggct gcaccgcagg tgactgcaga agacgcatct gccagcctgg
    12421 cagaactgct gaacgcaggt ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
    12481 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
    12541 tcagaatatt taaggaattt ctgaatactc ataatcaatg tagagatgac taatatcctg
    12601 aaactgactg aactaattga gtcaaactcg gcaaggattc gatactattc ctgtgtaact
    12661 ttcttaagga acgagaatga aacaggaagt ggaaaagtgg cgaccttttg gacatccgga
    12721 tggtgatatt cgtgatttat catttcttga tgctcatcag gctgtctacg ttcagcatca
    12781 tgagggcaaa gagcctttag agtatcgctt ttgggttacc tactctcttc actgcttcac
    12841 aaaagattat gaacatcaga cgaacgaaga aaaacaatcg ttaatgtacc acgcgcctaa
    12901 agaatctcgt cccttctgcc agcaccgtta taacttagcg cgcacacact taaaaagaac
    12961 tattttggcg ctgccagaaa gcaacgttat tcatgccggg tatggtagct atgccgtgat
    13021 tgaggtggac ttagacggag gagataaggc attttacttt gttgcgttca gggctttcag
    13081 ggaaaagaaa aaactccgtt tgcatgtaac tagcgcttat cccatttctg aaaaacagaa
    13141 aggtaaatca gtgaaatttt tcaccattgc ctacaactta ttgagaaata agcagcttcc
    13201 tcagccctca aaataacaaa acccacctta aggtgggttt cgccagagaa ttatctctgg
    13261 tattcagaac gccattaccg gactttgcct tgaccttgcg ataatcgcag gttgcgggat
    13321 gtctgaattt cttcagtctg ctgcatcctg gaagatgaga acatgtgttc ttattttcgt
    13381 ctctatcata gttgagtatt tactctctta caatcagatc tctttcattg ctcaacaggc
    13441 gatggcttca gactttgcat tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa
    13501 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13561 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    13621 ccgtctcata gtctttgcga ggcccgtccc agatagctgt cccagtcttt ccataccggc
    13681 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13741 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13801 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13861 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13921 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13981 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    14041 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    14101 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    14161 tgttttttgc ctttcagatg atggcgggca taagtcgcct cgtgatatgt ctcctgataa
    14221 accatcacgc catccagacc aagctgcttt aactcggcgt attccgtctt cgccagcggt
    14281 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    14341 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    14401 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    14461 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14521 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14581 cgttgggcca gttgttccag atagccactg gcggctggcg ataacagcgc catcatatcg
    14641 tcgcgggtga attgcgaggc atttagcgcc cgctctacgt caacagccgt tttgccgttg
    14701 atacgcaggc ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg
    14761 ccgatgcctc cagaaatcca gtcagcgggc tggtggcatg agcaaaatga ctgcggctgc
    14821 ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat gccttcgcca
    14881 tgttgacggg atcgtccgct acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14941 tttccagggc ctgcgctgca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    15001 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    15061 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    15121 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcatttcgg
    15181 cggctttcag agtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcta
    15241 attttaacca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    15301 ccgctgtttt cgccccggaa gtatttggca gcagggtcac acccgccgcg ataagcggtt
    15361 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    15421 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15481 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15541 ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgt ccactgctca
    15601 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15661 tccagtagtt cgtgaacagt ttgcccggtg gcgcactgca tcggttgatc gttaaacagg
    15721 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15781 ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggtgtct ctataccgct
    15841 taataactta atggcttcca gtgcctgcaa agtgcccata accccgacca ccgggccaac
    15901 cacgcccgcc gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta
    15961 acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct
    16021 ggcggtgata agcggcgtgt tgagtaccac gcaggcggca ttaatctcct ggcgagtcgc
    16081 catattgtcg gtacagtcga gcaccacatc ggcccgtgca accgcatctt ttaacgcctc
    16141 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    16201 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga gaatttgtcg
    16261 ttacagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    16321 gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa tcagcacctg
    16381 gctgtcgagc agtttttgct gcccgtcaag agcgatatca tcgagcagga tttggcggct
    16441 ataacgcata aaatcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16501 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgat gacggcgata ctgccgacac
    16561 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16621 aatccgccag tcgctcaaca tgccgtgcca gctgttccag cccctgcggt gcagaaggca
    16681 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16741 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacacc cagccgcagg cctgccgcgc
    16801 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16861 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16921 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16981 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    17041 ccactgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    17101 ttcctcctta cgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    17161 cattcccatt tcaatagttt gcgtggcggc gtaatcacgc acttcctggc tgattttcat
    17221 cgagcagaat ttcggcccac acatggagca aaaatgggcg actttacctg actcttgcgg
    17281 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    17341 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    17401 atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa gcccctgctt
    17461 aacatcttct ttattaggca gacccagatg ctcttttggt gttacgtaac agagcatcgc
    17521 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17581 cgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17641 ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc cttcaatcat
    17701 cacctgcacg tcatattccc aggcaatttt ggtcagttcg cccagcgtat gcagctcggc
    17761 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17821 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17881 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17941 accggtcagg cgtttcgcgg tcatcggcac atagcgcaac agtacgcccg catggatagt
    18001 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    18061 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    18121 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    18181 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    18241 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    18301 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    18361 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gacgtgcgcc
    18421 aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgcgct cgcggcccat
    18481 attctcgcgg atggcgatga attccatttc cggtgtgatg atgccctggc gggcgtagtg
    18541 cagttgggtg acacggcgtc ctgctttggc gcgttttggt gttagtacgc cgctaaaacg
    18601 cagttcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac tgcgcacggt
    18661 aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18721 ctgcacgtta atggcgatct gcggatcgcc atatgggccg gaggtgtcgt agaccggaat
    18781 cgcttcgttt tcttcaaact gcggctgttc tttgctgccg ccgattagcg tcgggctaag
    18841 ctggatctca cgcatcggca cgcgaacgcc agagtgtgtg ccagtgaggt aaatgcgttt
    18901 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18961 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    19021 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    19081 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    19141 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    19201 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    19261 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    19321 aaaacctttt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    19381 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    19441 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    19501 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    19561 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    19621 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    19681 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19741 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19801 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19861 ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19921 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca gcagcggcgt
    19981 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgtgctgtt tcaactggcc
    20041 ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    20101 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg ccgtgagcgt
    20161 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgatcctc
    20221 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    20281 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    20341 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    20401 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    20461 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    20521 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    20581 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    20641 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    20701 tatctaccgg aatataaagt cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20761 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20821 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20881 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataag
    20941 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    21001 cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    21061 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    21121 tatgccgatc cgcaggcgct gcacgctcta ctcgataaac tggcgaaaag cgtcactttg
    21181 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    21241 ggtgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    21301 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt taccaaaggc
    21361 ggcggacagt ggctggaagc gatggcagaa accggttgcg atgcgttggg cctcgactgg
    21421 acaacggata tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    21481 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    21541 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    21601 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacaa
    21661 tatcaccgct aaggagtgat tatggatctc gcgtcattac gtgctcaaca aatcgaactg
    21721 gcttcttctg tgatccgcga ggatcgactc gataaagatc caccggatct gatcgccgga
    21781 gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag cggcgatggt gctgctgaaa
    21841 tatccctcgc ttgagctggt ggagtataaa gttgcccgca tcgccaccac catgccttac
    21901 attccaggtt ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg ggagatgctg
    21961 tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga tctcgcatcc tcgccgtctt
    22021 ggcgtcgcca gccattttgg tttgatggtg gatgtgccaa cgatcggcgt ggcgaaaaaa
    22081 cggctctgcg gtaaattcga accgctctcc agcgaaccgg gcgcgctggc cccactgatg
    22141 gataaaggcg agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa cccgttgttt
    22201 atcgctaccg gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca acgctgcatg
    22261 aaaggctatc gtctgccgga gccaacgcgc tgggcggacg cggtggcctc ggaacgtccg
    22321 gcgttcgtgc gctatacagc aaatcagccc taattcaggt aaactgcgga taatttccgt
    22381 atttgagaac ttaacatgtt acaaaaccca attcatctgc gtctggagcg cctggaaagc
    22441 tggcagcatg tcactttcat ggcttgctta tgcgaacgca tgtaccccaa ttacgccatg
    22501 ttctgccagc aaaccggttt tggtgatggg caaatttacc gtcgtattct cgatctcatc
    22561 tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg acagccaact ggagaaattt
    22621 gaagaagcga ttccttcagc cgacgatttc gatctgtacg gcgtttatcc ggcaatcgat
    22681 gcctgcgtgg tgttaagtga actggtccat tcgcgtttga gtggtgaaac gctcgaacac
    22741 gcggtggaag tgagtaagac ctccatcacg accgttgcga tgctggaaat gactcaggtt
    22801 ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag ctgtagagca agaatgggac
    22861 attcagtggg aaatattccg acttttagct gagtgcgaag aacgcgatat cgagctgata
    22921 aaaggcctta gggcagacct gcgtgaggcg ggtgagagca atattggtat aatttttcag
    22981 caataagacc agaaaacgtg atttaacgcc tgatttgtcg tacctggagt cttccctttc
    23041 gccccccgtc tggtctacat ttggggggcg aaaaaaagtg gctatcggtg cgtgtatgca
    23101 ggagagtgct attctggcat ttccgtcgca ctcgatgctt agcaagcgat aaacacattg
    23161 taaggataac ttatgaacaa gactcaactg attgatgtaa ttgcagagaa agcagaactg
    23221 tccaaaaccc aggctaaagc tgctctggag tccactctgg ctgcaattac tgagtctctg
    23281 aaagaaggcg atgctgtaca actggttggt ttcggtacct tcaaagtgaa ccaccgcgct
    23341 gagcgtactg gccgcaaccc gcagaccggt aaagaaatca aaattgccgc agctaacgta
    23401 ccggcatttg tttctggcaa ggcactgaaa gacgcagtta agtaagattg cgtggcagtg
    23461 aacagtttta acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg atcattgatg
    23521 ctggctggcg ctctgcttct cactgcctgt agtcataact cttcacttcc tccctttacc
    23581 gccagtggat ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga cagcggcgat
    23641 aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg gcgataccac gacgcgagag
    23701 tatcgctggc agggcgataa cctcacgctc atcaatatca atgtttacag caaaccgccg
    23761 gtgaatattc gcgcgcgttt tgacgatcgc ggtgatctga gctttatgca acgtgaatcc
    23821 gatggggaaa agcagcagct ttctaacgac caaatcgatt tataccgtta tcgtgctgat
    23881 cagatccgcc agattagcga tgccttacgt caggggagag tcgtgctgcg ccaggggcgc
    23941 tggcatgcga tggaacagac cgtgaccacc tgcgaagggc aaaccattaa acctgattta
    24001 gattcgcagg cgatagcgca tatcgagcgc cgccagagcc gctcttctgt tgatgtcagc
    24061 gtggcatggc tggaagcgcc cgaaggttcg caattactgt tagtggcaaa ctctgatttc
    24121 tgtcgctggc aacccaacga gaaaacgttc tgatttacca gtggcccata cccatatgac
    24181 cgccaccacc gcagccgccg tagcccatgc ccattccggc tccgcgcgga atacccgctt
    24241 cagccatcgc gatatctcgt ttcacccgta actcatctaa cgactgacgc aaattctcca
    24301 tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat
    24361 tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt
    24421 cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg gcattttgct
    24481 gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa
    24541 gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg
    24601 tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg acgggtaatc
    24661 tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca
    24721 ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga tggcatgatt
    24781 tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc aacgttctaa
    24841 agactcctta gctaaatggt taagcgcgat cctccccgtg gtcattgttg ggctggtagg
    24901 gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag actgccgccg ccagacaaac
    24961 gctgctggaa aaaggcagtg tacttattcg cgctcttgaa tccggctcgc gcgtcggcat
    25021 ggggatgcgc atgcatcatg cgcagcagca ggccttactg gaagaaatgg ccgggcagcc
    25081 tggagtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc atagcaactc
    25141 cggcatggtg ggaaaacagc tttattcccc gcaggaaatg cagcagttac atccgggaga
    25201 tgaagaagcg tggcggcgga tcgatagcgc agacggtgag cctgttctgg aaatttatcg
    25261 ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg cgccatatgc agcagtatgc
    25321 cgcgacacca caagcaattt tcatcgcttt cgacgccagt aatattgtga gtgccgaaga
    25381 tcgtgagcag agaaacaccc tgattatcct cttcgccctg gcgacggtct tgctggcaag
    25441 cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc tacaagatga
    25501 aatgaagcgc aaagagaagc tggtggcact ggggcatctg gcggcaggcg ttgcccacga
    25561 aatccgtaat ccactttcct caattaaagg gctggcgaaa tactttgccg aacgcgcgcc
    25621 agcaggggga gaagcgcatc aactggcgca ggtgatggcg aaagaagccg accgtttaaa
    25681 ccgcgtggta agcgagttgc tggaactggt taagccaacg catctggctt tgcaggcggt
    25741 ggatctcaac acgctgatta accactcatt acagctggta agccaggatg caaacagccg
    25801 ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg ccgatccgga
    25861 caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga ttgttcagca
    25921 tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc gtgaaaatca gcgttaccga
    25981 cagcggtaag ggaattgcgg cagatcagct tgaagccatc ttcactccgt acttcaccac
    26041 caaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg aacaacacgg
    26101 tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc tctggcttcc
    26161 ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga tattctggtg
    26221 gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg ctggggctat
    26281 aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc aggtgcggga acaggttttt
    26341 gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac gctgaaagag
    26401 atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc cagcgtcgag
    26461 acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc gctggatttc
    26521 gataacctac aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat tgatgctgaa
    26581 acacctgcgg tgactgccag ccagttcggt atggtcggta aaagcccggc gatgcaacac
    26641 ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cagtgctgat ccacggcgat
    26701 tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa
    26761 aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga atctgaattg
    26821 ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg gcgctttgtt
    26881 gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc gatgatgcag
    26941 gtgcgtctgc tgcgtgcgat tcaggagctc gaagttcagc gtgtcggtag caaccagact
    27001 atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc agaggtgaat
    27061 gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat tgaagtacca
    27121 tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct gcagcgcttt
    27181 gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga tctgttgatt
    27241 cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg ggcagtggtg
    27301 ctgctgccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc agtacgccga
    27361 tcccgctggg gcaaagtcag gatattcagc cgctggtgga agtggaaaaa gaggtgattc
    27421 tggcggcact ggagaaaacg ggcggcaaca aaactgaagc cgcccgtcag ttagggatca
    27481 cgcgtaaaac gttattggca aaactgtcgc gttagttctg ctcgcgttcg atagcacgcc
    27541 agccgatatc tttccggcag aagcagtcgt cccagtggat atcggtcatt aaggcatagg
    27601 cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt acgcgcccgc
    27661 cgttggtcac tacctgctcg tcatccgcca gttttgtgcc cgcgtggaac actttgccgc
    27721 ctgccacttc ttccagcagc aggccgtgga tcacgtcacc ggtgcggtaa tcacccggat
    27781 atccacccgc agccatcacc acgccgagag aagcgcgttc atcccactcg gacgttttct
    27841 cgtccagttt gctttcacag gccgccaggc agagctcaac cagatcggac ttcatgcgca
    27901 gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt aaattcaata accttcggat
    27961 tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg
    28021 ccgccatgcc tttcacggtt ggccagataa tacgttccat ggtgcgctga tgaacgtcat
    28081 cggttactac cggcgcgggg gagtaagcgc ccatcccgcc ggtgtttggt ccggtatctt
    28141 tatcgcctac gcgtttgtga tcctggctgg tagccatcgg cagcacatgc tcgccgtcca
    28201 ccatcacgat aaagctcgct tcttcgccat cgaggaactc ttcgataacg atgcgatgac
    28261 ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcc gcttcttcca
    28321 gcgtcatcgc cacgataacg cctttcccgg cagccagacc gtccgcttta atgacgattg
    28381 gcgcgccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag ttctggtatt
    28441 ccgccgtagg gatcttatgg cgggccagga aatctttggt aaacgctttt gagccttcca
    28501 gttgggccgc acctgcggtt gggccgaaga ttttcagccc ggcggcgcgg aaggtatcga
    28561 ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc ttttcgtttt
    28621 gtgcgaaatc caacagcgcc gggatatcgg tcacgccaat agcaacgttt tgcagcgcgg
    28681 gttccagcgc agtgcctgca ttacccggag caacaaaaac agtctcaacc agcggcgact
    28741 gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact aatactttca
    28801 tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg caataccgtg
    28861 ctcgtcggcg gcggcaatca cttcgtcatc acggatggaa ccgccaggct ggattacgca
    28921 ggtcacgccc gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga
    28981 agccatcgag gaacctttca cttccaggcc ttcatcggca gctttaatac cggcgatttt
    29041 cgcggagtac acgcggctca tctggcccgc gccaataccg atggtcatat tgtttttggc
    29101 atagacgata gcattggatt tcacgaactt cgccactttc cagcagaaca gcgcatcgcg
    29161 cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg caccgaccat
    29221 ccccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat caagacccgg
    29281 aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat
    29341 tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact gacgagaaat
    29401 gatggcctgt gcggtttccg catctagctc gcggttaaag gcaatgatgc cgccgaatgc
    29461 ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat tgccgatagc
    29521 tacaccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga actctttcac
    29581 gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt taccctgaac
    29641 ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga aggcagcctg
    29701 ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca ggttcagcgt
    29761 gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg tacgccggaa ccatgctgcc
    29821 gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag
    29881 gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt taataatggc
    29941 gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag cggcggagcg
    30001 caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg agcagccttc
    30061 acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga taggctggat
    30121 ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc caccatgtac
    30181 tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga
    30241 aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc ctgtagacag
    30301 cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga
    30361 aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc ccctggattt
    30421 gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg
    30481 gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt
    30541 ttctctttca atggtgatca caattttgac agtggctacc gtgggaaaaa tacagaaatt
    30601 acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg
    30661 aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga gaactcccta
    30721 taatgaacct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    30781 gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta atatacgcca
    30841 cactcacgac agtgcgctaa agctcgtcgc aactgctctt taacaattta tcagacaatc
    30901 tgtgtgggta ctcgaagata cggattctta acgtcgcaag acgataaatg aataccaagt
    30961 ctcaagagtg aacacgtaat tcattacgaa gtttaattct ttgagcatca aacttttaaa
    31021 ttgaagagtt tgatcatggc tcagattgaa cgctggcggc aggcctaaca catgcaagtc
    31081 gaacggtaac aggaatcagc ttgctg
//
LOCUS       DAJTNN010000027        31059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTNN010000027 DAJTNN010000000
VERSION     DAJTNN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.027"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(5..3286)
                     /locus_tag="OO150_001120"
     CDS             complement(5..3286)
                     /locus_tag="OO150_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5515339.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISCYKNVPVTTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLVIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            3404..4597
                     /locus_tag="OO150_001121"
     CDS             3404..4597
                     /locus_tag="OO150_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5515340.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4661..5914)
                     /gene="glyA"
                     /locus_tag="OO150_001122"
     CDS             complement(4661..5914)
                     /gene="glyA"
                     /locus_tag="OO150_001122"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5515341.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6242..7432
                     /gene="hmpA"
                     /locus_tag="OO150_001123"
     CDS             6242..7432
                     /gene="hmpA"
                     /locus_tag="OO150_001123"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5515342.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIYYECFGP
                     HKVL"
     gene            complement(7477..7815)
                     /gene="glnB"
                     /locus_tag="OO150_001124"
     CDS             complement(7477..7815)
                     /gene="glnB"
                     /locus_tag="OO150_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5515343.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7876..9210)
                     /gene="glrR"
                     /locus_tag="OO150_001125"
     CDS             complement(7876..9210)
                     /gene="glrR"
                     /locus_tag="OO150_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5515344.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9200..9913)
                     /gene="qseG"
                     /locus_tag="OO150_001126"
     CDS             complement(9200..9913)
                     /gene="qseG"
                     /locus_tag="OO150_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5515345.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGCWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10078..11505)
                     /gene="qseE"
                     /locus_tag="OO150_001127"
     CDS             complement(10078..11505)
                     /gene="qseE"
                     /locus_tag="OO150_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5515346.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12063..15950)
                     /gene="purL"
                     /locus_tag="OO150_001128"
                     /gene_synonym="purI"
     CDS             complement(12063..15950)
                     /gene="purL"
                     /locus_tag="OO150_001128"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5515347.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16210..17766
                     /gene="mltF"
                     /locus_tag="OO150_001129"
     CDS             16210..17766
                     /gene="mltF"
                     /locus_tag="OO150_001129"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5515348.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17763..18299)
                     /gene="tadA"
                     /locus_tag="OO150_001130"
     CDS             complement(17763..18299)
                     /gene="tadA"
                     /locus_tag="OO150_001130"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5515349.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18324..18959)
                     /gene="yfhb"
                     /locus_tag="OO150_001131"
     CDS             complement(18324..18959)
                     /gene="yfhb"
                     /locus_tag="OO150_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5515350.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDKPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19168..20016
                     /locus_tag="OO150_001132"
     CDS             19168..20016
                     /locus_tag="OO150_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5515351.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20072..20332
                     /gene="yfhL"
                     /locus_tag="OO150_001133"
     CDS             20072..20332
                     /gene="yfhL"
                     /locus_tag="OO150_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5515352.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20526..20606)
                     /gene="shoB"
                     /locus_tag="OO150_001134"
     CDS             complement(20526..20606)
                     /gene="shoB"
                     /locus_tag="OO150_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5515353.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21026..21406)
                     /locus_tag="OO150_001135"
     CDS             complement(21026..21406)
                     /locus_tag="OO150_001135"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5515354.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21406..22137)
                     /gene="pdxJ"
                     /locus_tag="OO150_001136"
     CDS             complement(21406..22137)
                     /gene="pdxJ"
                     /locus_tag="OO150_001136"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5515355.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22149..22877)
                     /gene="recO"
                     /locus_tag="OO150_001137"
     CDS             complement(22149..22877)
                     /gene="recO"
                     /locus_tag="OO150_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5515356.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22889..23794)
                     /gene="era"
                     /locus_tag="OO150_001138"
     CDS             complement(22889..23794)
                     /gene="era"
                     /locus_tag="OO150_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5515357.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23791..24471)
                     /gene="rnc"
                     /locus_tag="OO150_001139"
     CDS             complement(23791..24471)
                     /gene="rnc"
                     /locus_tag="OO150_001139"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5515358.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24744..25718)
                     /gene="lepB"
                     /locus_tag="OO150_001140"
     CDS             complement(24744..25718)
                     /gene="lepB"
                     /locus_tag="OO150_001140"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5515359.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25734..27533)
                     /gene="lepA"
                     /locus_tag="OO150_001141"
     CDS             complement(25734..27533)
                     /gene="lepA"
                     /locus_tag="OO150_001141"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5515360.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27731..28210)
                     /gene="rseC"
                     /locus_tag="OO150_001142"
     CDS             complement(27731..28210)
                     /gene="rseC"
                     /locus_tag="OO150_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5515361.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28207..29163)
                     /gene="rseB"
                     /locus_tag="OO150_001143"
     CDS             complement(28207..29163)
                     /gene="rseB"
                     /locus_tag="OO150_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5515362.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29163..29813)
                     /gene="rseA"
                     /locus_tag="OO150_001144"
     CDS             complement(29163..29813)
                     /gene="rseA"
                     /locus_tag="OO150_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5515363.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29846..30421)
                     /gene="rpoE"
                     /locus_tag="OO150_001145"
     CDS             complement(29846..30421)
                     /gene="rpoE"
                     /locus_tag="OO150_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5515364.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30418..30573)
                     /gene="rseD"
                     /locus_tag="OO150_001146"
     CDS             complement(30418..30573)
                     /gene="rseD"
                     /locus_tag="OO150_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5515365.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30829..>31059
                     /locus_tag="OO150_001147"
     CDS             30829..>31059
                     /locus_tag="OO150_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5515366.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGI"
BASE COUNT         7425 a         8323 c         7834 g         7477 t
ORIGIN      
        1 gggtttagtg aacgtagtta aaaataaaag gcatcacagt ggtgaataat gccgctttcg
       61 gccaggccgg attaatcgcc gtcaattcag cgcgagcaga ctcatattgc gcaacctccc
      121 cgagtcccgc ggacgccagc atcgccacca tcaggcattt ttctttatgc tgctgttgta
      181 aatcgccata cagcgataac aggtcaggtt gtgaaacagc aaagaaatcc gcctcaatac
      241 tggttttcgc catctcttgc gcccactgtt tcatttcact aaacagttgc tgtgcggttt
      301 gctgttcacc cagcagtcgc agcgccattc cttgccagaa gagataatca accggctgat
      361 cgttgtaata actgtggatg ttaatggtgc gatcgccggt cgccgccaga cgtaaacaac
      421 gcatcgcttc agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag aaccagatgt
      481 cgttatcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa tgcagcgcgg
      541 catgaagcag ttcgctggcc tgctgcggct ctctggcatc aagatgctgc caggcgcgta
      601 ataactggtt gaggataaac tgactggtga ccttcccttc cccgccttcc cacgggtgga
      661 atttacgcgt ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga tgccacaaat
      721 tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta ttttccagac
      781 gcgccagtcg tttctccggt gtggctccac ttaacttatc aagcaaatcc cgttcgaaaa
      841 gcagacgtgc atcctgcggc gcaagctgat aagcattatc aagataacgc gcggccagct
      901 cataatcgtg ttgcttattc cacgcatgga tcgctaaccc gcgccagccg tcggcaaact
      961 ccggcgacat ctctacgcaa cgttgccaaa aggcaatggc tttgttgtag ctacgtttgt
     1021 tgtagtagaa gcaggccagt aaatggcgag caaaccagca ctcttcaata ctctccagcg
     1081 ccgccacttc ttccagcgta ttcgggaaac ggacaaactg cgggaagaca tcaatggctt
     1141 ttgcgaccag ttcgccacgt tcggctttcg gcagcaagct ggcttgcagg taaagcggca
     1201 gcgtgcgctg gcagtccagt gcgttcagca tctctgccgc cagggtgggc attccccagt
     1261 taatcagttg cccggcggtc atcagggcgt taacgtcgcg tccctgacac agaccgcgcc
     1321 actgcgcgag agctgattcg ctacgaccat cgaaccagtt cagccaccac agagtggcgt
     1381 tcagcggata atcgcgcagc agtttctcgc gctgcacacg cgcgttgtcc tgacgaccac
     1441 ttaacaccag cagcagatta tgcaggcaaa gcacttcctg attggttggg caggcgcgaa
     1501 gactttgttg gcaaaaatcc agaccagcgt cgaagttacc attacgcgcc gccagtcgtg
     1561 ccaggccata atagccaccg gctttgctgt tgccgctcca gaccgcacgc cagaaatcct
     1621 cttcggcttg ttgatattgt ccctgacgtt cgtaagcact ggcgcgaatc aaactcgcct
     1681 gtccgcactg cggatttttg ttcagcgcat gtgcgcgttt cagagcctga gtggcatacg
     1741 ccaccgcttg cgggaaatcg gcgcggttat attccagcat cgccagcgcc aggttacagc
     1801 ggtaatccag cggatccagc gccacgccgc gcaggtagta atcgaacggt gaacggctgg
     1861 cgtgatgata ttgctccaga tgctgaccga taaaccaggc ttcatctgta ctggtaatgt
     1921 cttgtgctgc cagtggcgct ttggcgacgt ccagcaacgg caactcttgc ggctgatgtt
     1981 cctgataact cagtacaata ttgccgtcgg catcggagag ctcaatggtc agcctttcag
     2041 gattgatacc gtgcaacacg ccctggatgg cggtcgcagg catcagtgcc acggcatcat
     2101 caagtaacgc gttgcatttg ccgatttcgc ggatcaccag gcggtatccg ttcaacggag
     2161 agatggcata cagcccccac tcaatccccc gcttactacg ctggagtttt atcaccgcat
     2221 cgcgggaggc attttgcacc atgcccaaag aatgataagg caggaaatac tgctcaaaac
     2281 gcttctcttc gtaagcatca agccaggtaa aatcgggctg gttatcggca aaaataccgg
     2341 tcatcagttc gatatacggg ccgttattgt cggtcagact cttatcccac gcctggccaa
     2401 attcactgtg tccccaactc cactgttttt tacctggcgc aatatggtgg ttggcaacgt
     2461 gcagcaaacc gccatcttca tcgtgacacc acgcgccgac aaaatcgtac tgcgattttt
     2521 cggccatata tgaggttgta acaggcacat ttttatagca agaaatgtcc actccggcgg
     2581 agtagtccac tttgtagtaa gtgccggtgg cgatggggaa agcggagacg gcccgtttgc
     2641 cgtgatcaaa caccgcagtc acatccggtg ggaaaacgct ctgatgccct tccccccctt
     2701 tcactgccgg gttggcccac cacaagaaat gacgcggcgt ggcgttaccg ttatagacgc
     2761 ggctggcgat ttccagcgcc gcccggtcag ggcgcagggt gaaacctgtc atcacctgta
     2821 aaccatgcat cggctccgtt tcgcctaccc acaccgtctg tgcaccgtct tcatgggctt
     2881 cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg atgttgcggc cagttaaact
     2941 caatcccacc ggaaatccac ggccccagca gccccaccag cgcaggttta atgacttcat
     3001 tgtgataaac aaaatcgcgc tgtttcactt tatcccacgc gcgatgcacc cgaccgccca
     3061 gttccggcag gatcatcact ttgatgtagt cgttttccag ccacaccgcc tgccaggatt
     3121 tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta gggataaacc gcgccggacg
     3181 atccctgata aacgcgattt tccaggaaca tgggatgtat atcctgcggc ccggtttcat
     3241 aggtcgggat ctcaacgcgc tcttgccaca cttttactgg agtcatggtg ccctcaatat
     3301 taacaagaca tactgaatta aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt
     3361 tcgcggaata atcacgcaat taactaaaca aggtttagtg aagatgagag cctgcattaa
     3421 caatcaacag attcgccacc ataacaaatg cgtgattctg gaactgctgt accggcaaaa
     3481 gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg cagtcagtaa
     3541 tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat attgacgatg aaagccagac
     3601 gcgcgggcat agtagcggta catggctgat tgcgccggaa ggtgactgga cgctgtgcct
     3661 gaacgtgacg cccaccagta ttgagtgtca ggttgctaat gcttgtttaa gtccgaaagg
     3721 tgaatttgag tatttacaga ttgatgcacc gacgccgcag gcgctgctgt ccgaaatcga
     3781 aaaatgctgg catcgccacc gtaaattgtg gccggaccgc accatcaatc tggcgctggc
     3841 aatccacggt caggttgatc cggtgaccgg cgtgtcgcaa accatgccgc aagcgccgtg
     3901 gacaacgccg gttgaggtga agtatctgct ggaagagaag ctgggcattc gggtgatggt
     3961 cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg caaaataatt cgcaggaacg
     4021 ggatttctgc gtgatcaacg ttgattacgg cattggctcg tcgttcgtga ttaacgagca
     4081 aatttatcgc ggcagtttgt atggtagcgg acaaattggt cacaccatcg ttaatccgga
     4141 tggcgtcgtc tgcgactgtg gacgttacgg ctgcctggaa accgtcgcct cgttaagtgc
     4201 attaaaaaaa caggcgcggg tatggctaaa atcacaaccg gttaatactc agcttgatcc
     4261 tgaaaaactg acaaccgcgc agttaatcgc tgcctggcag agtggagaac cgtggatcac
     4321 cagctgggtt gatcgctctg ccaatgccat tggtttgagt ctgtataact tcctcaacat
     4381 cctcaatatt aatcagattt ggttgtacgg tcgcagttgt gcctttggtg agaactggct
     4441 taatactatt attcgccaga caggatttaa cccgttcgac cgcgacgaag gaccgagcgt
     4501 gaaagcgacg caaattggct tcgggcaatt aagccgcgca caacaggtgc tgggaattgg
     4561 ctatttgtat gttgaggcgc agttacgaca gatttgatgg cgcgataacg tagaaaggct
     4621 tcccgaagga agcctttttt caacagatta accgtttcgc ttatgcgtaa accgggtaac
     4681 gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca tcattgatgc
     4741 tgtccagcac gtcacacatc cagccagcca gttctttcgc ttctgcttct ttgaagccgc
     4801 gacgcgtaat tgccggagta cccacgcgaa taccggaggt cacaaacggg ctcttcggat
     4861 cgttcggtac gctgtttttg ttgacggtga tgttagcacg gcccagagcg gcgtctgctt
     4921 ctttaccggt caggttttta tcaaccagat caaccaggaa caggtggtta tcagtgccgc
     4981 cggaaaccac tttgtagccg cgctcgagga acacttctac catcgcttta gcgtttttcg
     5041 cgacctgctg ctggtaagtt ttgaactcag gctccatcgc ttctttcaga gcaaccgctt
     5101 taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg gcagagttca
     5161 gttttttgta cagctcttcg ctaccacctt tcgccaggat caggccgccg cgcggacccg
     5221 ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg aggaaccggg ttcgggtaga
     5281 cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac gaacaggtaa gcaccgatgc
     5341 tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc ggaatatgca gagaaaccac
     5401 cgataatcat tttcggcttg tgttctttgg cttgtttttc cagatcggcg tagtcgatat
     5461 gaccggtagc atcgataccg taaggaacga tgttgtacag tttaccggag aagttaaccg
     5521 gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat acccagaacg gtatcacctg
     5581 gttccagcag cgcggtgtag accgcaaagt tagcctggga gccggagtgc ggctggacgt
     5641 tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt tcaacgatat
     5701 caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca tatttgttgg
     5761 tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg gaggcgatca
     5821 gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac agttcggcat
     5881 cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc agctaacaat
     5941 aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat aaccgaatca
     6001 ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc aggctacgca
     6061 aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg tttgtgatgc
     6121 aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt acattgcagg
     6181 gctatttttt ataagatgca tttgaaatac atcaattaag atgcaaaaaa aggaagacca
     6241 tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac tggtggaaac
     6301 ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc cagaactcaa
     6361 agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc tgtttaacgc
     6421 tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag cggtagaaaa
     6481 aatcgcgcag aagcacacca gcttccagat caaaccggaa cagtacaaca tcgtcggtga
     6541 acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc tggacgcgtg
     6601 gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg aaatctataa
     6661 cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca ttgtggctaa
     6721 aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg gtggcgcagt
     6781 ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag gtttcccgca
     6841 tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct atcgtattgc
     6901 ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg ccaatgttgg
     6961 cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt atggctgtcg cagatgacac
     7021 accagtgacg ttaatctctt ccggtgttgg tcaaacgcca atgctggcaa tgctcgacac
     7081 gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag aaaatggcga
     7141 tgttcacgcc tttgccgatg aagttaagga actggggcag tcactgccgc gctttaccgc
     7201 gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt ttgatagcga
     7261 aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa tgcagttcta
     7321 tctctgcggc ccggttggct tcatgcagtt taccgcgaaa cagttagtgg atctgggcgt
     7381 gaagcaggaa aacatttatt acgaatgctt tggcccgcat aaggtgctgt aatttgatgt
     7441 tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt cgtcctcttc
     7501 accggtacgg atgcgaatga cccgtgccac gtcaaagacg aagattttac cgtcaccgat
     7561 tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa tgtcgtccgg
     7621 tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg cgccgcggta
     7681 cagctcggta tggcctttct ggcgaccaaa gcctttcact tcggtcaccg tcatgccggt
     7741 aataccgact tcggccagtg cttcgcggac atcgtccagc ttgaagggtt ttataatcgc
     7801 atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt cttttgatct gctaaacgta
     7861 acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc gggaaagcag
     7921 tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga cgttgccttt
     7981 ggtaatttgc agcagcttac gcaaatagtt gagttcaaac tgattacgtg cctcaacaaa
     8041 ggttggcagc gccgtgtttt caccctccag cgcctgctct actaatgcat cgctaataac
     8101 cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc gcacattacc
     8161 cggccagcta gcggtcatca ggcgtttcat cgcatcagtg gagaacgcgc ggacaaacgg
     8221 tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agaagcggaa tgtcttctgt
     8281 gcgctctgcc agagccggaa ttttcaggct gacaacgttg aggcggtaat agagatcttc
     8341 gcggaattcc ccgcgcgcca tcgcttttgg cagatcacgg tgagtggcag aaataatccg
     8401 cacattgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct cctgcaacac
     8461 gcgcagcagt ttgacctgta acggcgcagg catatcgcca atttcatcga gaaatagcgt
     8521 tccgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag taaacgcgcc
     8581 acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc cacagttaat
     8641 agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg atagcctggg cgaaaatctc
     8701 tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg ccaccagccg
     8761 cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaattgcct cgcgccagcg
     8821 ttcatcggtg gctggcgcgg attgctccag cgcatcgtca attgcctgat atagcgcgtc
     8881 tttgtcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag caacggcatc
     8941 ggggatagaa ccatgcgcag taagaataat tactggcatt cccggctgca ctttctggat
     9001 ttcagcaaat agctgcatac cgtccatttc atccatccgc aggtcgctga taactaaatc
     9061 tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg tgaccacact
     9121 gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat cgtcatcgac
     9181 caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg gatggcgcgg
     9241 gcttttcact ctcgtgcggc gtatccggcg agaaatttcc agccggtttg cgggtcgaga
     9301 gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc agttgctgct
     9361 gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct gtcgctggac tgctggagtt
     9421 tactgtaacg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg tcgcgccaga
     9481 gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca atacgcgcga
     9541 ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc ccttgtttaa
     9601 aggtattttg ccagcagccg tcgtcatatt ggcgagcttg ctgacgcgac tgcgcaggca
     9661 ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt tcggttgatt
     9721 taccttgcag cgcccagatg tcgctacatt cagtagaaag atagtcagcc agttgataaa
     9781 ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc tgcacgcaac
     9841 ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat aatcgttgaa
     9901 aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat tgttgcagac
     9961 agtctggaca tggacagcgc ggagaaaccg gagcgtacat atcgtacgtg aagatttcga
    10021 gcactgcccg gagccgaact gacaaataaa atagcctgat aggataggct gttagcatta
    10081 tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc cgctctcgtc
    10141 gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc ttaatcccag
    10201 accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg gttcgaagat
    10261 catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa tataaacccg
    10321 cgcaccatgt gaactgctgc gaaggcaaat gttaccggat tcagccccgt agtgcaccgc
    10381 attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca ggcaagctgt
    10441 tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc tatgagcaga
    10501 aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg cactgtccgc
    10561 ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc ggctgctgct
    10621 atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa cctggtcagc
    10681 cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat gagataaatg
    10741 tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta accaaagaat
    10801 acgttgccca accgagcgta actcgctcgg tccactgaac gaaacgctat tgcccagaga
    10861 acgcccttcc cccagacggt tgatcatgcg ctcgatattt ttcaccggcc cgataatcat
    10921 ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg attgccaacc
    10981 aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag agaacaccac
    11041 tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa gacgcgcggc
    11101 ggcggcagca tcgggaccac tgttgttaca ctgaagttgg gccagattgt tcaaatcctg
    11161 acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat cgagcatttc
    11221 gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt ccagcacgca
    11281 atactgacgg tagctacgct ccatctccag cgccgcgttg gtcatcgctt cactacgccg
    11341 ggcatcaata agcgtagtgc ggttaaccag cgccgcctga tcgctaagcg cattcaggct
    11401 ttgccaggct tgccatgcca gcaccaacag gggtagtaga attagcaaaa atgccagcat
    11461 taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt tactctcgtc
    11521 agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag ccgggaatta
    11581 cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga tggttgatat
    11641 agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat tcgaatttta
    11701 tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgct tactattatg
    11761 cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg ttgctttgag
    11821 ggatttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct aaaaagagac
    11881 agcttaagat aaacttatta gattacaata aaatcaatca cttaagtgtc gccagaaagc
    11941 gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc ggagttggga
    12001 gcttatgata gtggttggtg cttaatgccg catccgggct gcaaaaccaa tgaactgatg
    12061 aattacccca actgcttacg cgcattgcgg aaaatgcgca tccatgggcc atcctcgccc
    12121 cagttttccg gatgccagga gttgctgaca gtacggaaaa cacgttccgg gtgcggcatc
    12181 ataatggtga ctcgaccact ttcagtcgtg actgccgtaa taccgttcgg tgaaccgttc
    12241 gggttggccg ggtaggtttc agtgactttg ccgaagttat cgacatagcg cagtgccacc
    12301 agccctttgc tttccagtgc cgccagatgc gccgcatcac gcacttccac gcgcccttca
    12361 ccatgagaga cagcaatcgg catttgcgag cccaccatcc cctgcaacaa cagagacggg
    12421 ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc gatcggaggt attgcgcaca
    12481 aaacgtggcc acaactcact acctgggatc agttcgcgca ggttagacat catctggcaa
    12541 ccgttacata cccccagcgc cagcgtttgc ggacggtgga agaaggttgc aaactcatcg
    12601 cgtacacggt cattgaacag gattgacttc gcccaacctt caccggcacc cagcacatca
    12661 ccgtaggaga aaccaccgca cgcgaccagg gcgtggaaat cttccaggcc cgtgcgtccg
    12721 gtcagcaggt cactcatatg cacgtcgata gcatcaaagc ctgcacggtg gaaagctgcc
    12781 gccatttcaa catgcgagtt cacgccctgc tcacgcagta cggcaacttt cggacgtgcg
    12841 ccagtggcaa tatacggtgc tgccacatct tcgttgatat cgaacgacag ttttacattc
    12901 aggcccggat cggcgtcgtt agatttcgcc tgatgctcct gatcggcaca ctccgggttg
    12961 tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc agacacgcaa cgtggtgcgg
    13021 ctttcgctga atacagtctg cccgttggcg gtaatcacaa aacggtcacc ggaaaccgcc
    13081 tgccctacat aatggacaca atcagcaagc ccatgctgtg ccagtacgga ctcgaccgct
    13141 tcacggtcag cggcacgaac ctgaatcacc gcacccagtt cttcgttaaa caacgccgcc
    13201 aggcgatcgt cgcccagaga ggcaatatcc gcgttaatgc cgcaatgacc agcaaaggcc
    13261 atttccgcca gcgttaccag caggccgcca tcagagcggt cgtggtatgc gagcagctta
    13321 cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt tcagttgcgc gacgtcgcgt
    13381 acatctgccg gtttgtcgcc aagctgacga taaacctgcg ccagcgccgt tgcgcccagc
    13441 gcgttattgc ctttgcccaa atcaatcagc aatagtgcgt tatcttcggt agaaagctgt
    13501 ggcgtgatgg tgtgacgcac atcttccacg cgggcaaatg cagaaatcac cagcgacagc
    13561 ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc gggttttcat cgacatagag
    13621 tctttaccca ccgggatcgt caggcccagc gccggacaaa gctcttcgcc cacggcttta
    13681 acggcttcat acaggcccgc atcttcacca gggtggcctg ccgccgccat ccagttggcg
    13741 gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg caatattggt taacgcttca
    13801 ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg caaccggcgc acgctcgcca
    13861 atcgccatcg cttcaccgta gtagctgtcg aggctggcgg tagtgaccgc gcagttagcg
    13921 accggcacct gccacggacc caccatctga tcgcgcgcta ccatgccagt tacgctgcgg
    13981 tcaccgatgg tcaccaggaa agttttctcc gccacggtcg gcagatgcag tatgcgtttc
    14041 accgcgtctg caatggtgat cccttcacgg accagcgcat cgcccttcgc tttcagcgtt
    14101 tgtacatcgc gggtcatctt cggcgtttta ccaagcagga cgtccagcgg cagatcgatc
    14161 ggctgattat caaaatgacg atcgtgtaga gaaagatgca gttcttcggt cgcttcacca
    14221 atcaccgcgt agggtgcgcg ctcacgctta cacagttcgt caaacagcgg taattgatcg
    14281 gcagcaaccg ccagcacgta gcgttcctgg gattcgttac accagatttc cagcgggctc
    14341 atgcctggct cgtcgctcag aatgtcgcgc agttcaaatt taccgccacg cccgccgtcg
    14401 ctcaccagtt ccggcatagc gttagaaaga ccgcctgcgc caacgtcgtg gataaacagt
    14461 attgggttgg catcaccaag ctgccagcaa cggtcaatca cttcctggca gcgacgctcc
    14521 atctccgggt tgtcgcgctg tacggaagca aagtcgaggt cggcatcaga ctgaccagac
    14581 gccatggaag acgccgcgcc gccgccaaga ccgatgttca ttgccgggcc gccgagaacg
    14641 accagcttcg cgccaacgtt gatctcgcct ttttgtacgt gatcggcgcg aatgttgccg
    14701 atcccgcccg ccagcatgat cggtttgtga taaccgcgca gctcttcgcc gttgtggctg
    14761 ttcacttttt cttcataagt acggaagtag ccgttcagtg ccggacgacc aaattcgttg
    14821 ttaaacgccg caccgcccag cgggccttcg gtcatgatgt ccagcgcggt gacaatgcgc
    14881 tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc caagaattcg caggttggaa
    14941 acggagaaac caaccaaacc cgctttcggc ttcgcgccgc gcccggtggc accttcatcg
    15001 cggatttcac cgccggaacc ggtcgccgcg cccggccacg gagaaatcgc cgtcgggtgg
    15061 ttgtgagttt cgactttcat cagaatatgc gccggttcct gatggaaatc atagcggccc
    15121 gtttcgtggt cagcaaagta gcggcccact tcagaacctt ccattacggc ggcgttatct
    15181 ttataagcag agagaacgtg atctggcgtg gtttcgaaag tatttttgat catcctgaac
    15241 agcgatttcg gctgttgttc accgtcgata atccagtcgg cgttaaaaat tttgtggcgg
    15301 cagtgctcag agttcgcctg agcgaacata tacagctcga tgtcgttcgg gttacgacca
    15361 agctttgtga aagcatcctg cagatagtca atttcatctt ccgccagagc caggccaaga
    15421 cgcaggttag cgtcgatcag cgcctgacgg ccctgcccca gcaaatcaac gcttgtgacc
    15481 ggagtcggtt gatggtgggc aaacaactgc tctgcatcat ctaaatcaaa aaagacagtt
    15541 tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc attgttcatt ggtcagcgta
    15601 ccggcttcta tatagtaagc aacgccgcgc tcaaggcggt ttacctgttg tagcccgcag
    15661 ttatgggcaa tatcggtcgc tttcgaagac cagggagaga tggtgccagg acgcggggtc
    15721 accagcagga gtttgccttg cggggcgtgg ctggcgagtg ccggaccata tttcagcagg
    15781 cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat tgaggtcagc aaaatggaca
    15841 tactcggcgt aaatattgtg aaccgggagc ctggcagcct gaaaacgtgc cagcagtttg
    15901 ttgattcgga atgccgacag tgcaggcgaa ccacgcagaa tttccatcat aagtctctcg
    15961 tcttctaagc tttcggtgta cccaaggggg ggggaaacgg gcgtcattat aaagaatctg
    16021 atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg
    16081 ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt
    16141 atgagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac gacacagaga
    16201 attaactaat tgaaaaaatt aaagattaat tatctgttca tcggcattct ggcactgctg
    16261 ctcgcggtcg ctctctggcc atccattccc tggtttggta aagccgacaa ccgtatcgcc
    16321 gccattcaag cgcggggaga gttgcgtgtg agcaccattc atactcccct gacttataac
    16381 gaaatcaacg ggaaaccttt tggcctggat tacgaactgg cgaaacagtt tgccgattac
    16441 ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca gccagctgtt tgacgacctt
    16501 gataatggta acgccgacct gctggcggca ggactggtct ataacagtga gcgggtaaaa
    16561 aattatcagc ctggccctac ctattattcc gtgtcacaac aactggttta taaagtgggt
    16621 cagtatcgcc cacgtacgct gtgcaacctg acggcggagc aactcaccgt tgcaccgggt
    16681 catgtggtgg ttaacgatct ccagaccctg aaagaaacaa aattcccgga attaagctgg
    16741 aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga aggaaaactc
    16801 gattacacca ttgctgattc tgtcgccatc agcctgtttc agcgcgttca cccggagctc
    16861 gccgtagcgc tcgatatcac cgatgaacaa ccggtgactt ggtttagccc gttagatggc
    16921 gataataccc tttccgccgc cctgctcgac ttcttcaacg aaatgaatga agacggtacg
    16981 ctggcacgca ttgaagagaa atacctgggg catggcgatg attttgatta cgtcgatacg
    17041 cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt taaagcccct gtttgagaaa
    17101 tacgccgaag aaattgactg gcgtttgctg gccgctattg cttatcagga atcgcactgg
    17161 gatgcacagg ccacttcacc gacgggtgtg cgcggcatga tgatgttaac caaaaatacc
    17221 gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac agagcatcag cggtggcgtg
    17281 cgttatttgc aggatatgat gagtaaagtg ccggaaagtg tgccggagaa cgagcggatc
    17341 tggtttgccc tcgctgcgta caatatgggc tatgcgcata tgctggatgc ccgcgccctg
    17401 acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg taaaacagcg tctgccttta
    17461 cttagccaga aaccctatta cagcaagctg acttacggct atgctcgtgg acatgaagcc
    17521 tacgcttatg tcgaaaatat tcgtaagtat caaattagcc tggtgggtta tctgcaagag
    17581 aaagagaagc aggctacaga agcggcgatg caactggcgc aggattatcc ggcggtatcg
    17641 cctacggagt tgggcaaaga gaaatttcct tttctctcgt ttctttccca gtcgtcatca
    17701 aactatttga cccattctcc ctctctgctg ttttccagga aagggagtga agagaaacaa
    17761 aattaatccg tcgaggattg cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga
    17821 aagaagtcac tgagcaacgc cgcgcactca tccgccagta ttccttccgt aatttccact
    17881 cggtgattca tacccggatg atgcagcaca tccattaaag atcccgcagc gccagttttc
    17941 gcgtcacgcg caccaaagac cacgcgacca atgcgactgt ggatcatcgc tccggcacac
    18001 attacacatg gttcaagcgt gacatacaac gtggcgtcga tcagacgata attttgcatc
    18061 accagaccac cctgccgcag ggccatgatt tctgcatgtg cggtgggatc atggcgacca
    18121 atcgggcggt tccaaccttc gccgattacc cgattgttat gcactaatac cgcgccgacc
    18181 ggcacttccc gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg catccagtat
    18241 tcgtggctaa attcgacttc agacaaaaag aaaactccgg ttataaaagc gcggcgcatt
    18301 atacacggac gctatgcttt actctattcc agttgctgga gttcaccgcg cggggttact
    18361 cgccaacgat gctgacagaa ataaagcagc ggattgtcct gattactgtc gctatagcca
    18421 ctgtacagcc gcagcggagt gccgattttg cgctccagtt gtgcgacctt ttcatgtccc
    18481 agacaacgca tcgtcaatac ccaaccacca tagccacgct gaatttggct ggcgataaga
    18541 ttaacccgcg gcagccaggg cttatcgaaa taaaccgctt caaccagcgg ctgcggagag
    18601 ccggtaatca accagatatc agcatcggaa cttaacaggt aggtggttaa tcgctcctga
    18661 accagcggaa aggcggtaac attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc
    18721 tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca gaagcagact catcggccag
    18781 cgtgccgcac gaccttttat caataacgca atggctataa tcggtaacaa cggcaggaca
    18841 agtaacgcat tcagcggttg gcgacgtagt aaatagcgca gaaaactgcc gaacatatcc
    18901 tgctgatgca atgttccatc taagtcaaaa aacaccacac gacgctcgtg agttgccaaa
    18961 ccatactcct ctggactgaa acatcctgat taattttcgc tactacatag cctaacagat
    19021 agatcatcac ttttccggca acaatcgacc gcatcatggc taactggaat ttttaattca
    19081 tggcaattag cggcaatgga atataaaatt cactcgcgtg tgtctcatat tgcctgttgt
    19141 cgccactatt tcatcgcgag gaaagatatg aacggcttac ttcgtatccg tcagcgttac
    19201 caggggcttg cccagagtga taaaaaactg gcggattatc tgctgctaca acctgatacg
    19261 gcgcgccatt taagctctca gcaactggcc aacgaagccg gagtcagtca gtccagcgtc
    19321 gtgaagttcg cgcaaaaact cggctataaa ggttttccgg cgcttaagtt ggcgttgagt
    19381 gaagcgctgg caagccagcc ggaatcgccc tccgtgccca ttcataacca aatccgcggt
    19441 gatgatccgt tacggctggt cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac
    19501 gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct
    19561 gcgcggcgga taattctgac cggtattggc gcttcgggtc tggtggcgca aaactttgcc
    19621 tggaagctga tgaagattgg cttcaatgct gccgcagtgc gcgatatgca tgcgctactc
    19681 gcaacagtac aggcgtcgtc ccctgacgat ctgttattag ccatttccta caccggtgta
    19741 cgacgcgagt taaacctggc ggcagatgag atgctgcgag tgggcggaaa agtgctggcg
    19801 attaccggct ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct gtataccatt
    19861 gccgaagaac aagcgacaaa cagtgcttca atctctgctt gtcacgctca gggaatgtta
    19921 acggatttgc tgttcattgc gctgattcag caggatctgg aactggcacc agaacgtatt
    19981 cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc
    20041 gcccggtttg tgttgttttg agagtttcct tatggcgttg ttaatcacta aaaaatgcat
    20101 caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg atttcaatgg gagatcatat
    20161 ctacgagatt aacagcgata agtgtaccga atgcgtaggg cactacgaga caccaacctg
    20221 ccagaaggtg tgcccgatcc ccaatactat tgtgaaagat ccggcgcatg tcgagacaga
    20281 agaacagttg tgggataaat ttgtgctgat gcaccacgcg gataaaattt aatattctac
    20341 tctggaagta gagtattaat tatattactg ggaagcctta acgccattat atttatttaa
    20401 ttgatgacat tagcataatc attcactaag ttaatttata tagtatctgc caagacactt
    20461 atttatagtt attaaaggcg cgtccgattg gttcaccgga cgcaccttaa gtacgtttcc
    20521 ttgtgttata agaacagaag gatcagctgt aaaacagcaa tgatgatttt gatgacccgt
    20581 ttaatcaggt atcggcaatc agtcattcgt ttttccttaa acaaggaatg cagccgttca
    20641 gattagccct tacatctccc caaaactgaa cgtgcgagtt attgagggtg catgctgcac
    20701 tccacaccag agctttgacg acaccactcg tttcaatggg ggaattctgt ggcatggtgt
    20761 aaagcacagc aaaatcttca ataacgaagc caattttaat gtacttacga attggcggtc
    20821 acgttaatct ttccatcaat attgctttct tcgtaaaggc tcgagttttt atgctaaaga
    20881 ttgcaagttg cttgtaaaag ataagtacac tgatccataa tcgctgttgt tgagggtgca
    20941 tgctgcacaa aattaaagtt aaaaagtaaa acccccgttc cttaccagtt cgggggtttt
    21001 actttttaaa gagaacggta ttattttaac tttcaataat taccgtggca caagcatagt
    21061 ggcgttcatc tgccagcgtt acatgcatat ttacaacgcc gagcttttcc gccagtttta
    21121 atgcctcacc ccatagccgt agccgtggtt tgccgagctc atcattgaat acttcaaatt
    21181 gattaaacgc cagaccattg cggatcccgg tgccaaacgc ttttgctgcg gcttctttca
    21241 cagcaaaacg cttcgccaga aaacgcaccg gctggtggtg cgttttccag attgcccatt
    21301 cgttatcgct taatacgcgg cgtgctaggc gctcaccgga tcgggcgatc actgcttcta
    21361 tgcgagcgat ctctacgata tccgtgccta aacctaatat tgccattagc cacgcgcttc
    21421 cagcatcaga cgcttcattt ctgccaccgc atctttcagt ccggtcatca ctgcacggcc
    21481 aataatggca tgaccgatat tcagttcatg catctcaggg atggcggcaa tggctttcac
    21541 gttgtgatag gtcagaccgt gtccggcgtt aactttcaga ccgagactag cggcaaaggt
    21601 cgcggctttg gcgatacgca ccagctcttg cgcctgttcg gcgtcagttt tggcatcagc
    21661 atagcaaccg gtgtggatct cgataaacgg tgcgccaacc tctgccgcag ctttgatctg
    21721 ctcttcatcg gcgtcgataa agagagaaac ctgaatcccg gcatctgtca gacgtttgca
    21781 ggcatcgcgc attttttcac gctgccctgc aacgtccagg ccgccttcgg ttgttacttc
    21841 ctgacgcttt tccggtacca ggcagcaaaa atgtggcttc gtctcaacgg cgatcgccag
    21901 catctcttcg gtcactgcca tctccagatt cattcgggta tccagcgtct gccgcaggat
    21961 gcgcacatcg cggtcagtaa tgtggcggcg atcttcacgt aaatgcacgg taatgccgtc
    22021 cgctcccgcc tgctcggcaa taaacgccgc ctgcaccgga tccgggtaag cagtaccgcg
    22081 tgcgttacgc agcgttgcaa tatggtcaat gttgacgcct aacagtaatt cagccatgac
    22141 aatcctcatc attcataatg tgttttcacc gttcgcttag gcataaactg ccggaacagt
    22201 tccctgctct ttaatggttt accgccaaga tacggcttaa gcgccatgcg ggtaaagcgt
    22261 ttcgcggcgc gtagtgtgtc tgcgtcagga aattcccgcg cgtttaacgc ttttaactgc
    22321 cttccggtga acgttttatt gtcgataacg acgcttgcga taaacccttt ttcttcgcga
    22381 taacgatacg tcatggtgtc atctaccggc tcgccgctac ccgcgcaata ggtaaaattg
    22441 acgccatagc ccagatgccc gagcaatgcc agttcaaagc ggcgcagcgc gggttctggc
    22501 gtaccagtgg cacctgcaag agactgaatg cagtgcaaat aatcgaaaaa gagttcagag
    22561 aagcgcgtct cgtattccag tacgcgggag agaagttcgt tgatgtacag accgctgtaa
    22621 agcgtgatac cgcttaatgg cagcgccagc gagacagctt cagcactgcg cagcgttttg
    22681 acttcgccac gcccgccgaa acgtagcaag agaggggtga aaggctgtaa tgcacctttc
    22741 agagtagagc gtttagagcg tgcgcctttg gcaaccagac gcacgcgacc cgattcctcc
    22801 gtgaagacgt ccagcatcag gctggtttcg ctccacgggc gactatgcag gacaaatgcg
    22861 cgctgccagc cttccatcgg agtgactctt aaagatcgtc aacgtaaccg agactgcgca
    22921 gtgcgcgttc gtcgtcggcc caaccggatt tcacttttac ccacagttca aggtggacag
    22981 gcgcttcgaa catttcctgc atgtctttac gcgcttcaat cccgatggtt ttgatcttgg
    23041 cccctttgtt gccaataacc atcttcttct gcccttcacg ctcaacgaga atcaaaccat
    23101 tgatgtcata accaccgcgt tcgttagaga cgaaacgttc gatctccacg gtcacggagt
    23161 acggcagttc agcgccgagg aaacgcatca gtttttcgcg gatgatttca gacgccataa
    23221 aacgctgtga gcgatcggtg atgtaatctt ccgggaagtg atgggtcgct tcaggcagat
    23281 gcttacgcac gattgctgcg atagtgtcga cattcagccc ggtttcagca gagatcggca
    23341 cgatatcgag gaagttcatc tgacttgcca ggaactgtaa gtgcggcagc agatcggctt
    23401 tttcctgcac gttatccact ttgttcaccg cgaggatcac cggcgctttg ccatcgcgca
    23461 gtttgttgag caccatttcg tcgtccggcg tccagcgggt gccttcaacg acaaaaatca
    23521 ccagctcaac gtcgccaata gagctgctcg ccgctttgtt catcaggcgg ttaatagcgc
    23581 gtttttcttc catatgcagg ccaggggtat caacgtagat cgcctgatac gcgccttcag
    23641 tatggatccc cacaatgcgg tgacgagttg tttgcgcttt gcgggaggtg atggagatct
    23701 tctgccccag cagtttgttc aacaatgtgg atttgccaac gttcggacgt ccgacgatgg
    23761 caataaatcc gcagtaactt ttatcgatgc tcattccagc tccagttttt tcaacgcctg
    23821 ttcggcggca gcctgctcag ccttacgacg gcttgaacct gtgccaacca ccggttcact
    23881 caggccgctg acctggcagt ggatagtaaa ttcctgatcg tgcgcttcgc cacgtacctg
    23941 gactaccaga taagtcggca gcggcagatg gcgaccctgc aaatattctt gcaagcgcgt
    24001 tttcggatct ttttgtttat cgcctgggct aatttcgtcc aaacgagttt gataccagtt
    24061 gaggattaat ttctcgacgg tttgaatatc actgtcgagg aatacgccac caattaatgc
    24121 ttcgacggta tcggcgagaa ttgactcacg acgaaatcca ccgcttttaa gttcaccagg
    24181 ccctaaacgt aagcactcgc ctaactcaaa ttcgcgcgcc agttccgcca gcgtattgcc
    24241 acggaccagc gtggcgcgca tccggctcat atcgccttca tccacacgag ggaaacggtg
    24301 ataaagcgca ttggcgataa cgtagctcag aatagagtcg cctaaaaatt ctaaacgctc
    24361 gttatgtttg ctgctggcac tacgatgagt taatgcctgc tgcaacagtt cctgatgatt
    24421 aaaagtgtag cccagcttcc gttgaagccg attaattacg atggggttca tgcgatacca
    24481 ataaataaat gcgtcaacaa ttcagcacac gaaacagacc tgatatacat ggctctgcta
    24541 actgcttcgc tgcagtttca gtttaccggt atatggggac caacgctgtt tcgtgtgccg
    24601 tggcaacctg gaggtgccaa ccttaaactt cgggggaata ttctatacac aacgacgggg
    24661 gatgtcgtta gccacgggag atttatctca taaataattc acgttgtcgc cataacggcg
    24721 acaacgtgaa cgaagatggg ctattaatgg atgccgccaa tgcgacttaa gcgcacacct
    24781 gtcggccatt cgccttcttg cttatcgaaa ctcatccaga tagccgttgc ccgaccgacc
    24841 agattcgctt caggcacaaa accccagtaa cggctgtccg cgctgttgtc gcggttgtcg
    24901 cccatcatga agtattgtcc cggcggaaca atccaggttg ccagttgttg ccctggctgc
    24961 tggtaataca tccccacctg gtcctgcgca atcggcactg tcagaattcg gtgcgtcaca
    25021 tcacccagtg tctctttacg ctcggaaaga cgaattccat tttctttggt ttcgtttttc
    25081 ggcacttcaa agaatccgct ggtcgcttcc ccaccattac ggcgtgagaa ggtctgaacg
    25141 aaatcgctcg gttccacgtt ggagtaggtg accggtagcg cgttttcaca cgcctggccg
    25201 gaactgcatc ccggttgaat cgtcagctct tttgagaccg gatcgtaagt gactttatcg
    25261 cccggtaaac ccaccgcgcg cttgatgtaa tcaagctttg gatcttccgg atatttaaag
    25321 accacgatat cgccgcgttt cggatgaccg gtttcgatca gcgttttctg gtagatagga
    25381 tctttaatgc cataagcaaa cttctctacc agaataaaat caccgattaa cagagtcggc
    25441 atcatcgaac ctgacgggat ctggaacggt tcataaataa acgaacgtac aatcaatacg
    25501 atagccagca ccggaaaaac agaagcaccg gtttccagcc agccaggctt cggcgcaacc
    25561 tttttcaggg ttgctttatc cagtgagtca ccggcagccg cctgcgccgc tgcctgacgt
    25621 tcccgccgtt taggtgcgaa aaagaattta tccacgcacc ataaaatgcc cgtcaccagt
    25681 gtggcaatca ccagaatcag ggcaaacata ttcgccatgc caactcctaa gggttatttg
    25741 ttgtctttgc cgacgtgcag aatggcgagg aacgcttcct gcggcagctc gacgttaccg
    25801 atctgcttca tgcgtttctt accttctttc tgcttctgca gcagcttttt cttacggctg
    25861 atatcgccgc cataacattt agccagtacg tttttacgca gctgtttcac ggtggatcgc
    25921 gcaataatgt gcgtaccaat cgctgcctga atggcgatat caaactgctg gcgtgggatc
    25981 agatctttca tcttctccac caactcgcga ccgcggtttt gcgaattatc acggtgggtg
    26041 atcagcgcca acgcatcaac acgttcaccg ttgattaata cgtctacacg taccatgtcg
    26101 gacgcctgga agcgtttgaa gttgtaatcc agagacgcat aaccacgcga ggtagatttc
    26161 aggcgatcga agaagtcgag caccacttcc gccatcggga tctcgtacgt cagcgccacc
    26221 tgattaccgt ggtaaaccat attggtctgc acgccgcgtt tttctacgca cagcgtaata
    26281 acgttgccga gatatgcctg cggcagcagc atgtgacact ctgcaatcgg ctcgcgcagt
    26341 tcgtaaatgt tatttaccgc aggcagcttg gatgggctgt cgacgtagat aacttccctt
    26401 gacgtggttt caacttcata cactacggtc ggcgcagtgg tgatcagatc cagatcgtat
    26461 tcacgttcca gacgttcctg gatgatctcc atgtgcagca ggccaaggaa gccgcagcgg
    26521 aaaccaaagc ccagcgcgct ggagctttcc ggctcataga acagtgaggc atcattcagg
    26581 ctgagtttac ccagcgcgtc acggaaggct tcatagtcgt cggaacttac cgggaacaga
    26641 ccggcgtata cctgcggttt gactttctta aagccaggca gcgccttttc tgccggatta
    26701 cgcgccagcg ttaaggtatc gccgactgga gcgccgtgga tatctttaat cgcacatacc
    26761 agccagccta cttcgccaca tttcagttca gtgcggtcaa cctgtttcgg cgtgaagatg
    26821 cccagacggt cggcgttata ggtctgcccg gtactcatga ctttcacttt gtcgccctta
    26881 cgcagggtgc cgtttttaat acggataagt gaaacaacgc ccaggtagtt gtcgaaccat
    26941 gagtcgataa ttagtgcctg caacgggcct tccggatcgc cttccggcgg cggaatgtcg
    27001 cgcaccagac gttcgagaac gtcctgcacg ccaacgccgg ttttcgctga acagcgcacc
    27061 gcgtcagtgg cgtcgatgcc gacgatatct tcaatttctt ccgccacgcg ttcaggatcg
    27121 gctgccggca ggtcaatctt gttcagtact ggcaccactt cgagatccat ttccatggcg
    27181 gtgtagcagt ttgccagggt ttgcgcttcc acgccctgcc cggcgtcgac caccagcaat
    27241 gcaccttcac aggcagccag cgaacgggaa acttcatagg agaagtctac gtggcccggg
    27301 gtgtcgataa agttaagctg ataggtttcg ccgtcagacg ctttgtagtc cagcgtcacg
    27361 ctttgcgctt tgatggtaat gccacgctca cgctcaagat ccatggaatc gagaacctgc
    27421 gcctccattt cacggtcaga caggccaccg cagatctgga taatacggtc agacagcgtc
    27481 gatttaccgt ggtcaatgtg agctatgatc gaaaagttac gtatattctt cataaagtat
    27541 gattattgtg ccttaatgcc cggttaacca ggcttttaga agtcgctgtt ctgagcttaa
    27601 cgtctgtatt aatagaaacg ccgcattcta cactacaaca ttgaggcgag gaaatgttca
    27661 taccgtatgg attgtggtat ctggaaacgt cctcgcattt gttatgcaaa atgcaacaaa
    27721 gccagtgaaa tcactggctc gcgtcttccg aagatgtttc aaatcgcaca aggccaggcg
    27781 gcaatgccac gcttaagatg atcggttgcc attctgcccg ggcagcaaac ttacgcgagt
    27841 agccgcgcgc aatcaggaac ccgccaatac cgccgaggac cgcaccacat aatgctgcga
    27901 cgtcagaagc aaagagtagc tgaaatagcg aagcgatgag gaataatccc accagcggcg
    27961 acatataaac cagtaatgcg gagctaagca ggctgccttc ggcgatcccc aattccactt
    28021 tttgccccgg cactaacggt tcatcacagg gtacgacaat ggtatgcgtg gtttgcgggc
    28081 caagtttatt caacacgcgg ctaccgcaac cggcgcgtga agcgcagctg ctgcatgaag
    28141 ctttaacatc acaactgacc agcgcctgcc cgttttgcca ggagacgacg gtagcccact
    28201 ctttgatcat tgcgctgccc cgaacttaat attctcggca atgcgtttcg ccgtttgcgg
    28261 cggcagttca ccgacaatgg tgatttcggc gttatcacgt acgcttgtac tgacggttct
    28321 gcgtccggtg cgcaacatct gatcggtgct cgatggcgta gcgcggttaa cgtttaccga
    28381 gaagctgaat aatccgtcag aatagagacg tgattcgata ggcatgttgt ccatcgtcgg
    28441 tagcggacgt cgactactgg aaacttcgct aaaaccctgt ggcaaccagg ttggcgtcca
    28501 gctgaattta gctttttcac ctacaggaac agaaagcaac ggcggcaaat ttgccttcgc
    28561 cagcgtctgc atactgctgc tgatatcctg attgacgtta aaagcaatca cgcgaaattg
    28621 ttccagcgtt tcaccatcgc gatcaaggag atcaacccgc atcggtaatt tcgattcggt
    28681 gtccatccac acgatgtagc tgtagcgtgt accatctcgg gcaaccacgc gaatgacttc
    28741 gcaaagacga tcagcaatac gcgtgcgccc gacggagata aagtcgtagt aaggagaaag
    28801 gcgtttgaaa tcggtataga tgagcgatgg cagagaatca acaatgtaat cgccattaag
    28861 cgtgaacggt tcaagtcccg gttcaaaata gctgatttca tcgccgcgct gtaccacttc
    28921 ccggcgcggg ccatccattt gcaacaattg tgcaagagga cggttatcga ggcgtgcatg
    28981 tcgataacgc agagactcaa caccctgttt attgatgctg atgaatgaca gctcgtaatt
    29041 cagtgactga ctggccaggt tcatctgctg taataacgcc ccggacgcgg gagtggccga
    29101 ggcgttagca gagaataaca ggctacctgt cactaatgac atggcaaacc aaagttgctt
    29161 cattactgcg attgcgttcc taaagtttga attcctggca cctgtacagc ggcttgctgg
    29221 gtttgcgcct gctcaaactg aagctgttca gagtggagtc ggcgttgcag ttcgtaatcc
    29281 tgcaacattg cattaatgcg acgacgctgc tcctgtacct gctgctgttg accattgttt
    29341 gcggtcgctt cagaaggtac tcccaggctt accgggctgg ctttacccat catcggcagt
    29401 gtattaaata ccggcgtttc gggctgctgg gacgtttcag attgtccatt atagtgctgg
    29461 acgccaacga taactgcaag cgatacgcat gctgctacgc ccatttgggt aagctgtgcc
    29521 gcccacggac gtactttctg ccagaatggc attttctgcc attgatgcgg cgcaggctgg
    29581 gcttccggga tcaatgtcgc cggttgacgt actggctctt cttcaatggc ggccatcacg
    29641 cgtgaagaga tatcgaaatg gagcacctcg ggagtatcac cccgcattga gtcacggatt
    29701 aagtgatagc tttcccaggt tttctgcatt tctgggttat gagccagttc gttaagcagc
    29761 tcactatcca gcgtttcgcc atccattaaa gcggaaagtt gttctttctg catgcctaat
    29821 acccttatcc agtatcccgc tatcgtcaac gcctgataag cggttgaact ttgttatcaa
    29881 tagcttccct cgctcggaag atacgtgaac gcaccgtacc taccggacaa tccatgatag
    29941 cggctatctc ttcatagctc aggccatcca gctcccgcaa ggttattgcc atgcgcaaat
    30001 cttccgggag ggactcaata gttcggaaaa ctatctgtct cagttcttct gacaacatta
    30061 agttctcagg gttcgaaatt tctttcaacg cgccgccact ttcgaagttt tcagcttcaa
    30121 tggcatccac atcactggaa ggtggacgac gcccctgagc aaccaggtaa tttttcgctg
    30181 tatttacagc aatccgatac agccatgtat aaaaagcgct atctccccgg aacgaatcca
    30241 gcgcacgata ggctttaata aaagcttctt gtaccacatc gggaacatca cccgacggca
    30301 catagcggga aaccagactc gccactttat gctgatagcg cactaccagt aagttaaagg
    30361 ctttctgatc tcccttctgg acccgttcaa ccaggacctg gtccgttaac tgctcgctca
    30421 tccgaggtaa agtctcccca aaccaaattt ccacgcgcta tcgaaacgcc actccattag
    30481 ctgcaatttg agcaagcaaa gggttagagt gtctcgtttt tgtaaagttc cgtaacgcat
    30541 ctgtttttgt ttgtcatgct gtagacggat cattatctat cattataagt ctacagaatc
    30601 tgaacatcgc attatctgtg tagaaatgcc catttaactg cctgaagagt aacccaacgg
    30661 cctttttatt tcaccaccta atcctccacc agccagtaac ttctcttttt ctcgccgccc
    30721 tgcatcagcg tgtttagcaa ctgtaacaaa tattaaaata gcaggtgttt attcgcacaa
    30781 catgatgcta tactgaccaa accgtgttta gtaaattaaa caaagaaaat gaatactctc
    30841 cctgaacatt catgtgacgt gttgattatt ggtagcggcg cagccggact ttcactggcg
    30901 ctacgcctgg ctgaccagca tcaggtcatc gttctaagta aaggcccggt aacggaaggt
    30961 tcaacatttt atgcccaggg cggtattgcc gccgtgtttg atgaaactga cagcattgac
    31021 tcgcatgtgg aagacacatt gattgccggg gctggtatt
//
LOCUS       DAJTNN010000028        30781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTNN010000028 DAJTNN010000000
VERSION     DAJTNN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.028"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..144
                     /locus_tag="OO150_001148"
     CDS             <1..144
                     /locus_tag="OO150_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5515367.1"
                     /translation="GFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLIDNKIV
                     ELA"
     gene            complement(436..1332)
                     /gene="rpnC"
                     /locus_tag="OO150_001149"
     CDS             complement(436..1332)
                     /gene="rpnC"
                     /locus_tag="OO150_001149"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5515368.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQGKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            1606..1986
                     /gene="panD"
                     /locus_tag="OO150_001150"
     CDS             1606..1986
                     /gene="panD"
                     /locus_tag="OO150_001150"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5515369.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(1990..3190)
                     /locus_tag="OO150_001151"
                     /pseudo
     CDS             complement(1990..3190)
                     /locus_tag="OO150_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(3285..3725)
                     /locus_tag="OO150_001152"
     CDS             complement(3285..3725)
                     /locus_tag="OO150_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5515370.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(3830..4600)
                     /locus_tag="OO150_001153"
     CDS             complement(3830..4600)
                     /locus_tag="OO150_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5515371.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVASVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(4597..5523)
                     /locus_tag="OO150_001154"
     CDS             complement(4597..5523)
                     /locus_tag="OO150_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5515372.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKLETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            5632..6294
                     /gene="can"
                     /locus_tag="OO150_001155"
     CDS             5632..6294
                     /gene="can"
                     /locus_tag="OO150_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5515373.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(6335..6871)
                     /gene="hpt"
                     /locus_tag="OO150_001156"
     CDS             complement(6335..6871)
                     /gene="hpt"
                     /locus_tag="OO150_001156"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5515374.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            7077..9467
                     /gene="gcd"
                     /locus_tag="OO150_001157"
     CDS             7077..9467
                     /gene="gcd"
                     /locus_tag="OO150_001157"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5515375.1"
                     /translation="MAINNTGSRRLLVTLTVLFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPTEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPKLKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(9514..11064)
                     /gene="cueO"
                     /locus_tag="OO150_001158"
     CDS             complement(9514..11064)
                     /gene="cueO"
                     /locus_tag="OO150_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5515376.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            11230..11577
                     /locus_tag="OO150_001159"
     CDS             11230..11577
                     /locus_tag="OO150_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5515377.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            11683..12549
                     /gene="speE"
                     /locus_tag="OO150_001160"
     CDS             11683..12549
                     /gene="speE"
                     /locus_tag="OO150_001160"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5515378.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            12565..13359
                     /gene="speD"
                     /locus_tag="OO150_001161"
     CDS             12565..13359
                     /gene="speD"
                     /locus_tag="OO150_001161"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5515379.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(13396..13758)
                     /locus_tag="OO150_001162"
     CDS             complement(13396..13758)
                     /locus_tag="OO150_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5515380.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(13933..16530)
                     /gene="acnB"
                     /locus_tag="OO150_001163"
     CDS             complement(13933..16530)
                     /gene="acnB"
                     /locus_tag="OO150_001163"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5515381.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGLLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFMNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            16885..18738
                     /locus_tag="OO150_001164"
     CDS             16885..18738
                     /locus_tag="OO150_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR5515382.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAAVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYRMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(18809..20233)
                     /gene="lpdA"
                     /locus_tag="OO150_001165"
     CDS             complement(18809..20233)
                     /gene="lpdA"
                     /locus_tag="OO150_001165"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5515383.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(20558..22450)
                     /gene="aceF"
                     /locus_tag="OO150_001166"
     CDS             complement(20558..22450)
                     /gene="aceF"
                     /locus_tag="OO150_001166"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5515384.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(22465..25128)
                     /gene="aceE"
                     /locus_tag="OO150_001167"
     CDS             complement(22465..25128)
                     /gene="aceE"
                     /locus_tag="OO150_001167"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5515385.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(25289..26053)
                     /gene="pdhR"
                     /locus_tag="OO150_001168"
     CDS             complement(25289..26053)
                     /gene="pdhR"
                     /locus_tag="OO150_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5515386.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            26594..27967
                     /gene="aroP"
                     /locus_tag="OO150_001169"
     CDS             26594..27967
                     /gene="aroP"
                     /locus_tag="OO150_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5515387.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(28010..28864)
                     /gene="ampE"
                     /locus_tag="OO150_001170"
     CDS             complement(28010..28864)
                     /gene="ampE"
                     /locus_tag="OO150_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5515388.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(28861..29412)
                     /gene="ampD"
                     /locus_tag="OO150_001171"
     CDS             complement(28861..29412)
                     /gene="ampD"
                     /locus_tag="OO150_001171"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5515389.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            29500..30393
                     /gene="nadC"
                     /locus_tag="OO150_001172"
     CDS             29500..30393
                     /gene="nadC"
                     /locus_tag="OO150_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5515390.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            30596..>30781
                     /locus_tag="OO150_001173"
     CDS             30596..>30781
                     /locus_tag="OO150_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5515391.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGG"
BASE COUNT         7417 a         8386 c         7548 g         7430 t
ORIGIN      
        1 ggcttccgcg ccgatgatat tcagattcgc gatgccgaca cgttgctgga agtctctgaa
       61 accagcaaac gagctgtaat tctggtagcc gcctggcttg gcgatgcccg cctgatcgac
      121 aacaaaatcg tcgaactggc gtaatgcttt tactctaaat aattcaagtt gcaggaaggc
      181 ggcaagcgag tgacaaattc gtcaggaacg aatttgccca gccgcaggct ggccttcggt
      241 gagagacatg gatgtctctc attaatcccc aggagcttac ataagtaagt gactggggtt
      301 agcgaacgca gacacagcac atgcaattta aagtatgacg agtataactg gcgctacggc
      361 tgatggcgcc aattattaat ttgccccacg tcatggcgat ggcgtctgta agcaattatt
      421 cataacgcag acaacttagt taatcagcct atcaatctca gcaagagata aatttgtcat
      481 ctctgcaacg tcttcccgag acatcccttt actcagaaaa cgctgggcga attcttgcct
      541 ttcctcctgt cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc
      601 cagcgtcatc atagactccc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc
      661 cgcttgttca gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc
      721 gctagtgtac ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg
      781 ctggcgaata tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc
      841 gtcatccggt gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgtgc
      901 cagctccggc gagtaaaata tatcaaacca gcacattgag agcggataag gtgtggcctc
      961 gccctgataa aacagtatcg gcaccaccag cggcagctta tcgtgatccg cctccagatg
     1021 ccggtgcatg gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt
     1081 gccttggtgt tcaatcacaa catgcagata accgggattg ccctgcattt gcacggaata
     1141 gagcacgtcc gtgctgtgtc ctttcaggct ctcttcaatg aaactccccg actctaaatg
     1201 aagcgtgttg aggtcacaaa gttctcgtaa ttccactggc aaatgtatct ccagaaagtc
     1261 gcgagccgtc tccgcgtgca ttaaaaattg tttaaacact gcatcatgcg gtgtggtact
     1321 cggtgcatcc atcttccctg ccttccgtgt tgtcttgcgt gaaggcagta tccagtcaag
     1381 gcgtgccatc gtcagccacc tctttagtgt ttaccgagca gcgttcagag aaatttcaat
     1441 tggttacagc cgttacatgg cacgcttcgg cacggtacgc aaactgctga tgtaaatgcc
     1501 gtcagatgag tagacactaa acaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc
     1561 aggcatcgcc tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg
     1621 ctgcagggca aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct
     1681 tgcgccattg accaggattt tcttgacgca gccggtattc tcgaaaacga agccattgat
     1741 atctggaatg tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt
     1801 tcgagaatta tttctgttaa cggtgcggcg gcccactgcg ccagtgtcgg cgatattgtc
     1861 atcatcgcca gcttcgttac catgccagat gaagaagctc gcacctggcg acccaacgtc
     1921 gcctattttg aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt
     1981 gcttgattgt tatccctgcg gctggttact caccagccgc gacatcgtct ccagcgaatc
     2041 cgttcttaag atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc
     2101 tttcatggtt gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc
     2161 attaaatccg ccaaacggat acgaaagata ccagacatgc ggattaaatt gcgccagagc
     2221 gcggcgtgaa cgtgcaaaat caaacagaat attgtgttca ctacggctcg gtaatatggg
     2281 gcggcgataa ccatccaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc
     2341 gcgaatttcg ttaagttcag aaacgctcat aaattgcagc gattttgggt cccacttctg
     2401 cgggtgacgt ttgatgcgcg aggtaacaat aaacgccgtc gccttcatgc catattgttt
     2461 caacacggga tacgcatagc ggctcaccga tttgaggcca tcatcaaagg taatcaccac
     2521 cgctcgcgca gggagattga tcttattctt cacgtaacct tccagctgca ccatgctcag
     2581 tgtcgcgtat cccctgtcgc gcagccaggc catctggtta ttgaaagcgc gtactgaggt
     2641 ggtcgtcgaa gtatggcgaa aacgggtgtt ttcttcatcg cgcagaatat ggtgataggt
     2701 tagcaccggc aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc
     2761 gccaatacgg atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg
     2821 gtagcgcaaa ttgtccgcca gtaccccata atggcgcact gcccgcactc ggcgcgttat
     2881 agaccggcgt atctttccag gtaattaagt tctgattact cagcggcttg ttgaggtcac
     2941 ccaaaccgtc ttcaactttt tgtcgcccct gaactggctc gagatgccct ttatcgataa
     3001 accctttacc aaagccaaaa ttaaatgcgt aataacttgc ggcagtgggc tccacggcaa
     3061 tgatttgccc ggcccgaata ttccccacgg tcaccatctt gtcgccaatt tgcgcccaga
     3121 tcgcagcatt ttcgatggtt tgcatataac gggcaggtaa cgcggcacta acgcttgcgg
     3181 taaacagcat cagcaggatg aataacagct tgtttataca tgaaaaaata accgagggtg
     3241 aaacaacgct tgtattgtag caaaagcgcc atcagaatcg cgggttaata ctcatacaaa
     3301 ttatgtttga ggacaaaagg cggatttttt tgttgttggt gccaaagact actcacctcc
     3361 ggcgcaccca gttcgacaat acgctcgcga aactctgaac tggtcatggt ttcacggcaa
     3421 gccatcatct gttcaattaa cggtaacgtg acaccagaaa tcacttcaca acgggagtgt
     3481 ttatggctta ataatgaagc cacgcggtac ggtggcgctc cggctatatc ggttaagaag
     3541 atgacaccct caccagagtc cgcttcatgc agagcatcgc acatcatgcg gctgagcata
     3601 ttagagctta atccgcgcca gaaattcacg gcccggcact gaagaagtgc cccatgtttt
     3661 ttctccagcg catccagtat ctcttgcgcc cggtcatcgt gacaggtaat tacccaacct
     3721 aacatcgcct tcctccttag ccgaaaatca gtctatcctg accattgttg ataaagggtg
     3781 atagccgtca aaactcctct cccccgaatc cagaggagag gaaatagcct tagctacgca
     3841 aaccacgtcc acgttggatc agcgaccaac agatcaaata aaacaccaca ataaagacca
     3901 ccagtacgcc aaaggtagtg gccagcggca catcatttat accgaggaag ccgtagcgga
     3961 aaccactgat catataaacg attgggttca ggtgcgacag cccttgccag aacggcggca
     4021 acaaagtcag ggagtaaaac accccgccca aatacgtgag tggcgttaac acaaaggttg
     4081 gcaccaggct gatgtcatcg aacgttttgg caaacacgcc gttcagcaaa cccgcaaggg
     4141 agaatagcac cgccgtgagc accagggtta aggcaacgaa tacccacgaa tgcacctgaa
     4201 acggcacaaa aaacagtgaa attgccgtca ccagaatgcc aacaaacaga ccacgcgcca
     4261 cgccaccgcc gacatatccg gcaataatga cgtgagtcgg aaccgacgct accagcagct
     4321 cttcaatatt acgctggaac ttggcaccaa aaaatgatga cgcgacgtta gcgtaggcat
     4381 tggtgatcac cgacatcatg atcagccccg gtacgatgaa ctgcatatag ctgaagccat
     4441 gcatatcgcc aatacgcgaa cctatcaggt taccgaagat aataaagtaa agggtcatgg
     4501 tgatgactgg cggcaccagc gtctgcaccc agatacgcat aaagcgatgg atctctttcg
     4561 cccagatgct ttttagcgcc acccagtaaa gatgcatcat gcgcgatctc cttgtttttc
     4621 attaaccagt gaaacaaaca gctcttccag acggttagct ttgttacgca tacttaatac
     4681 ctgaatgccc tgctcactta actgcgtaaa tacgctgttg atcccctgct cacgcagcac
     4741 ttcaacttcc agcgtcgcgg tatcgaccag tcgatactga tagccatcga gcttcggtaa
     4801 cgggcttttc ggtgcgagat cgagaataaa ggtttccaat ttcagcttcg ccagcagcgc
     4861 cttcatcgag gtattttcca ccagctcacc gtgttgaata atgccgatat tgcggcacag
     4921 catttctgct tcttccaggt agtgtgtagt gagaatgatg gtggtgcctt tgtcgtttaa
     4981 atccttcaaa aagccccaca ttgagcggcg aagttcaata tccacgcctg cggtcggttc
     5041 gtcgagaatc agtagtttag gttcatgcat taacgcacgg gcaatcatta aacggcgctt
     5101 catcccgcca gataacatac gcgcacgttc gttgcgtttt ccccataaat cgagttgttt
     5161 gagatatttt tcgctgcgga tgtacgcttc tttgcgctcc acgccgtagt accctgcctg
     5221 attcaccaca atttgctgca cggtttcaaa cgggttgaag ttaaattcct gcggcaccag
     5281 tcccaactga cgtttagcgt tcacgacatc cttctcgaga tcgtaaccaa atacgctgac
     5341 ccgcccggag gttttattta ccagagagct gataataccg atagtggtcg atttcccggc
     5401 cccgttcggc ccgagaagcg cataaaaatc acccgcttcg acctgcaaat ctattccacg
     5461 aagcgcctga acgccgcctg gataggtttt tttaagctgt tgaagttcca gtgcaatggt
     5521 cataaatttt tacttacctt acgttcttac actttatctg tggtttaaat cgtcccggag
     5581 ttgccctata ttagccaaac gtaattattt ggttacaggt cgttaacctc catgaaagac
     5641 atagatacac tcatcagcaa caatgcacta tggtcaaaaa tgctggtgga agaggatccc
     5701 gggttttttg agaaactggc acaagcgcaa aaaccgcact ttctatggat tggatgttcc
     5761 gacagtcgcg ttcctgcaga acgtttaacc ggtcttgagc cgggcgaact ctttgttcac
     5821 cgtaatgttg ctaacctggt cattcacacc gacctgaact gcctttccgt ggttcagtat
     5881 gcagtggatg tactagaagt tgaacacatt attatctgtg gccactacgg ttgcggcggc
     5941 gtacaagccg cagttgaaaa cccggaactg gggcttatca acaactggct gctgcatatc
     6001 cgcgatatct ggttcaaaca tagctcattg ctcggcgaaa tgccgcaaga gcgccgtctg
     6061 gataccttgt gtgaactgaa cgtcatggaa caggtgtata acctaggcca ctccaccatt
     6121 atgcaatcag cgtggaaacg cgggcagaaa gttaccattc acggctgggc ctatggcatt
     6181 cacgacggct tgctgcgtga tctggatgtt actgccacca accgcgaaac ccttgagcaa
     6241 cgttaccgtc acgggatttc caacctcaag ctgaaacacg ccaaccacaa ataaaaatgc
     6301 catgccggat gcaacacatc cggcaacttc acacttactc gtccagcaga atcactttgc
     6361 cgatatacgg cagatgacgg taacgctgtg cgtaatcaat gccgtaaccc accacaaact
     6421 catccgggat cgagaaaccg ataaattcta ccgggacgtt cacttcacga cgggacggtt
     6481 tatccagcag cgtacaaatc gccagcgact tcggttcgcg caggcttaag atctcacgca
     6541 ctttcgacag tgtattcccc gagtcgatga tatcttcaac aatcagcacg tccttgccac
     6601 ggatatcttc atccagatct ttgaggattt tcacatcacg ggtggtggac atgccgctac
     6661 cgtagctgga ggcggtcata aagtcgactt catgagatac ctgaacttca cggcacaggt
     6721 ccgccataaa cataaatgag ccacgcagca gacccaccag caccatatcg ctgccgctgt
     6781 ctttgtaacg ctcagtaatc tgacgaccca gttcggcgat acgcgcttta atctccgctt
     6841 cggggatcat tacttctaca gtatgtttca tatctctaac catatgattt aaaaacaaat
     6901 cacttcgcac gggcaggttt tggcaccgca acaaagcgca agacacgcag tataccagta
     6961 aaactattca ttcgcggaat ctgttaataa agctcgaatt gtgatgacga tcacacatgt
     7021 taaaacctat aattaattgc tactaaaata ttaatgaatt gaaatggtgt ctctttatgg
     7081 caattaacaa tacaggctcg cgacgattac tcgtcacgct aacagtcctt tttgcagcgc
     7141 tttgcgggct gtatctactc attggcggag gctggctggt cgcgattggc ggctcctggt
     7201 actaccctat cgctggcctt gtgatgctcg gcgtcgcctg gatgctgtgg cgcagtaaac
     7261 gtgccgcgct ttggctatac gccgccctgc tgctcggcac catgatttgg ggcgtctggg
     7321 aagttggttt cgacttctgg gcgctgactc cgcgcagcga tattctggtc ttcttcggca
     7381 tctggctgat cctgccgttt gtctggcgtc gcctggtcat tcctgccagc ggcgcagttg
     7441 ccgcactggt ggtggcactg ctgattagcg gtagtatcct gacctgggcc ggatttaacg
     7501 atccgcagga aatcaacggc accttaagcg ccgatgccac acctacggaa gctatctcac
     7561 ccgttgccga tcaggactgg cctgcctatg gtcgcaatca ggaaggtcaa cgcttttcac
     7621 ctctgaaaca aattaacgcc gataacgtcc ataatctgaa agaagcctgg gtgttccgta
     7681 ctggcgatgt gaagcagccg aacgatccgg gcgaaatcac caatgaagtg acgccgatta
     7741 aagtgggcga caccctttac ctgtgtaccg ctcaccagcg cctgtttgcg ctcgatgccg
     7801 ccagcggcaa agagaaatgg cattacgatc ctaagctgaa aactaacgag tctttccagc
     7861 acgtaacctg ccgtggcgtc tcttatcatg aagccaaagc agaaacggct tcgccggaag
     7921 tgatggcgga ttgcccgcgt cgtatcattc ttccggtcaa tgatggtcga ctgattgcga
     7981 ttaacgctga aaacggcaaa ctgtgcgaaa ccttcgccaa taaaggcgtg ctcaatctgc
     8041 aaagcaatat gccagacacc aaaccgggtc tgtatgaacc gacttcgcca ccgattatca
     8101 ccgataaaac catcgtgatg gcaggttcag ttaccgataa cttctcaacc cgcgaaacgt
     8161 ctggcgtgat ccgtggtttt gatgtcaaca ccggggagct gctgtgggct tttgatcccg
     8221 gcgcgaaaga tccgaacgca atcccgtctg acgaacacac ctttaccttt aactcgccaa
     8281 actcctgggc accagcggcc tatgacgcga agctggatct ggtctatctg ccgatgggcg
     8341 tgaccacgcc ggatatctgg ggcggtaacc gcacaccgga acaggaacgt tatgccagct
     8401 cgattctggc gctgaatgcc actaccggga aactggcgtg gagctaccag accgttcacc
     8461 acgacctgtg ggacatggat cttccggcac agccgacgct ggcggacatc accgttaatg
     8521 gtcagaaagt gccagttatt tacgctccgg cgaaaaccgg caacattttt gtgctcgatc
     8581 gtcgcaatgg cgaactggtg gttccggccc cggaaaaacc ggtgccgcaa ggtgctgcga
     8641 aaggcgatta cgttactccg acccagccgt tctctgaact gagcttccgt ccgaagaaag
     8701 atttgagcgg tgcggatatg tggggagcca ccatgtttga ccaactggtg tgccgcgtga
     8761 tgttccacca gatgcgctat gaaggcattt tcacgccgcc atctgaacag ggcacgctgg
     8821 tcttcccggg taacctggga atgtttgaat ggggcgggat ttccgttgat ccaaatcgtg
     8881 aagtggcaat tgccaaccca atggcactgc cgtttgtttc gaaactgatc ccacgcggtc
     8941 cgggtaaccc gatggagcag ccgaaagatg ccaaaggcac gggtacggaa tccggcattc
     9001 agccacagta cggtgtaccg tatggtgtca cgctcaaccc gttcctctca ccgtttggtc
     9061 tgccgtgtaa acagccagca tggggttata tctcggcgct ggatctgaaa accaatgaag
     9121 tggtgtggaa gaaacgtatt ggtacgccgc aggacagtat gccgttcccg atgcctgtac
     9181 cggtgccgtt caatatgggt atgccgatgc tgggcgggcc aatctccacg gcgggtaacg
     9241 tgctgtttat cgccgctacg gcagataact acctgcgcgc ttacaacatg agcaacggtg
     9301 aaaaactgtg gcagggtcgt ttaccagcgg gtggtcaggc gacgccaatg acctatgaag
     9361 tgaatggtaa gcagtatgtg gtgatctccg caggcggtca cggttcattt ggtacgaaga
     9421 tgggcgacta tattgtggct tatgccctgc cggatgatgt gaaataacca taatgcccgg
     9481 agagatccgg gcatatttcc gaatacggtc tttttatacc gtaaacccta acatcatccc
     9541 cgtatcttca tgctccagca gatggcagtg cgccatataa gcatgttctt tcggtgcgtc
     9601 gtgattaaac ttcaccagca cttcactgac attgccttct accttaacgg tatctttcca
     9661 gcccgcgcga tgagccgctg gcggtttgcc attttctgac aagatacgga actgcgtgcc
     9721 gtgaatatgg aacggatgca gcatcatgtc gcccacgcca gagataaccc aacgttcgta
     9781 ctgccctttc gccgccgcaa acatcggctt attcatatca aacgcctgac cgttgatttt
     9841 attggcatgg tggaaatcga acttcccgcc gtggttcata tgattcatat taccgtgccc
     9901 catatggccc atcatctggc tgtgatccat tccggccatc gcctgatcgc catatttctc
     9961 cattagcatc tgcataccca tcatatcgag catcgggtcc atagaaagtt gcagcttgcg
    10021 taccgtcagc ccttccagcg aaggtaacgc aggcaggcta cttaatgtgt ctggcaaagc
    10081 accggaggca ctaatagcaa tcggctgaat ccgcattacc ggatgcggct tgtcaaacgg
    10141 cgcaatcgcc atccccatct ggctgaccgg tagcgtcacc aggtcaaagg gtttgttgtc
    10201 gttaacctcc accagcactt caaaacgctc gcccatcagc accggcagct cgctcacctt
    10261 caccggttca ggtagcagac caccgtcgct ggcaatcaca tacagcgggc gattgtcgct
    10321 ggtggcgaaa ttgagcgagc gggcattaca gccattgagc aaacgcaggc gcagccaacc
    10381 acgcggggca gcgtgttgtg ggtagattgc accgttggtc agcagcgtat cgccaaacca
    10441 gcccacggcg gcggtcatca catccagttg ataatcaatc tgcccatcag cgttaaattt
    10501 tttatcctga acaatcaccg gaacatcatc gataccccac tgttttggca gcattaattt
    10561 caggatctcg tcatcttcaa ttaccaccag cccagccaac cccatcgcca cctgtcgccc
    10621 agtcttgcca tgttgatgtg gatggaacca gcaagtagcg gcaggttgat caacgttcaa
    10681 cgtcaccgag cgcttgccac ctggcggaat aattccctgc gggccgccgt cgacttcacc
    10741 cggtacttcc agcccgtgcc agtgcaacgt cgtctcttcc gtcagttggt tatagatatc
    10801 aaccgttacc gctttgccgc gctgtaattt caccgccggc cccagcagat tgccgttata
    10861 gccccaggta gttgcagttt tctcgccaaa ggtagactga cctgcgccaa tagttaactg
    10921 aatgcgatta cgggcatcgg tcgtgagcaa atcagggatc ggtaacgttg ggcgttccgc
    10981 cgcaaagact gcgcggctcc acagcggcaa ggctgaagcc acacccagcg cgacggaata
    11041 ttttaagaaa tcacgacgtt gcatagttat ttccttattc ttaagcaggc gaaacaaaat
    11101 caaacgttga gcatagtcct tccccttacg ggaaggtcaa gccgcagaca caatttaatc
    11161 gacaataatg gtcgtcgcct cgctcgcagt gtgctaacgt ttatcttctt taagcccctg
    11221 gtagaagcaa tgaagacgtt tttcagaaca gtgttattcg gcagcctgat ggccgtttgc
    11281 gcaaacagtt acgcgctcag cgagtctgaa gccgaagata tggccgattt aacggcagtt
    11341 tttgtctttc tgaagaacga ttgtggttac cagaacttac ctaacgggca aattcgtcgc
    11401 gcactggtct ttttcgccca gcaaaaccag tgggatctca gtaattacga caccttcgac
    11461 atgaaagccc tcggtgaaga cagctaccgc gatctcagcg gcattggcat tcccgtcgct
    11521 aaaaagtgca aagccttggc ccgcgattcc ttaagcctgc ttgcctacgt caaataatcc
    11581 ctgatacctt tttgtagaaa tattgaccgt gcatccgcgg tcaatgttag ctattatgtt
    11641 gcgccctttt tttacgggtg ttaacaaagg aggtatcaac ccatggccga aaaaaaacag
    11701 tggcatgaaa cgctacacga ccagtttggg cagtactttg cggtagataa cgttctgtat
    11761 catgaaaaga ccgatcacca agatctgatc atttttgaga acgctgcatt tggtcgcgta
    11821 atggcgctgg atggcgtagt acaaaccacc gagcgcgacg agtttatcta tcatgagatg
    11881 atgacccatg ttccgctact ggcccacggt cacgcgaaac atgtgctgat tatcggcggc
    11941 ggcgacggtg ccatgctgcg tgaagtaacc cgacataaaa acgttgagtc aatcacgatg
    12001 gtggaaatcg atgcgggtgt cgtgtcgttc tgccgtcagt atctgcccaa ccataacgcc
    12061 ggtagctacg acgatccgcg ctttaagctg gtgatcgacg atggcgtcaa tttcgttaat
    12121 caaaccagcc agacctttga tgtcattatc tccgactgca ccgatcctat cggtcccggc
    12181 gaaagtctgt tcacctcagc attttatgaa ggctgcaaac gttgcctgaa tcctagcggt
    12241 atcttcgtcg cacaaaacgg cgtctgcttt ttacagcagg aagaagccat cgacagccat
    12301 cgcaaactca gccattactt cagcgacgtt ggcttttatc aggcggcgat cccgacctat
    12361 tacggcggta tcatgacttt tgcatgggcg acagataacg acgccttacg ccatctctca
    12421 accgaaatta ttcaggcgcg ttttctcgct tctggcctga aatgccgtta ttacaatccg
    12481 gcaatccata cggcagcttt tgccttacct cagtatctgc aagatgcact ggcttcacag
    12541 ccgtcctaag gagaagataa gaaattgaaa aaactgaaac tgcatggctt taataatctg
    12601 accaaaagtc tgagtttttg tatttacgat atctgctacg ccaaaactgc cgaagagcgc
    12661 gacggttata ttgcttatat cgatgaactc tataatgcca accgtctgac cgaaatcctg
    12721 tcagaaacct gttccattat cggggccaat attcttaaca tcgcccgcca ggattacgaa
    12781 ccacagggtg ccagcgtcac tattctggtg agtgaagaac cggttgaccc gaaactcatc
    12841 gacaaaacag aacaccccgg cccactgcca gaaacggtcg ttgcccatct cgataaaagt
    12901 catatttgcg tacataccta cccggaaagc catcctgaag gtggtttatg taccttccgc
    12961 gccgatattg aagtctctac ctgcggcgtg atttctccgc tgaaggcgct gaattacctg
    13021 atccaccagc ttgagtccga tatcgtaacc attgattatc gcgtgcgcgg ttttacccgc
    13081 gacattaacg gtatgaagca ctttatcgac catgagatta attcgattca gaactttatg
    13141 tctgacgata tgaaggcgct gtatgacatg gtggatgtaa acgtctatca ggaaaatatc
    13201 ttccatacca agatgttgct taaagagttc gaccttaagc actacatgtt ccacaccaaa
    13261 ccggaagatt taaccgacag cgagcgccag gaaattaccg ctgcgctgtg gaaagaaatg
    13321 cgcgagattt attacgggcg caatatgcca gctgtttaac ggctctggcg gagctccagg
    13381 ctccgccaga tttatttact tctgctgcac gaaattgcgg taagccgcca cgacctgcag
    13441 aaaatcctca acgccgcata gcgacaggct ttcttcgtcg tagtagttca tcccctcttc
    13501 catttcatcg ccagcgaatt ccagttgatt ggcgcgaacc atcacctctt caccgtccat
    13561 ccacagggtg tattcatgcc ctgcccgttg ccaggaccgt tcgctacctt tcagtgcgtg
    13621 cgctgcttgt tccacttcat caagcaaggc caggttttct ttcacctctt cattaaacca
    13681 gtgcccgacc acttcatgcc ccatggacat acgcaccttt accactccgg taatatcgcg
    13741 cagaaattcg taatccatag tgtgttcctc tgctcccggc aatgcgccga aatcgcattg
    13801 cgccattgat gtaattatcg cagccttacc ggagaaaaac agcggaacgg cgatggttta
    13861 gaaataaaaa atgcccggga atctcattca ccgggcattg tgtcgtttat gcgcagcgcg
    13921 tgcgcttact tattacaccg cagtctggaa aatcacccca tcggcttttt cggtgtactg
    13981 agaaagttgg ttgaagttca gataacgata agtatcaacg gcagttttat ctacttgcgc
    14041 aacgtaggtc tggtactctt ccggcgtcgg cagtttgcca atcagcgccg caacagccgc
    14101 cagttctgca gaagccagga agacattcgc gccagtcccc agacggttcg ggaagttacg
    14161 ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac ccatacacag
    14221 agaacagccc gggatctcga tacgagcccc gctcttaccg aagacgctgt agtagccttc
    14281 ttcagtcaac tgtgcggcgt ccatacgggt tggcggtgcc acccacaggc gggtcggcaa
    14341 ctggccttta tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga tgttagtcat
    14401 gcaagaaccg ataaatactt cgtcgatctt ctcgccctgt accgcagaca gcggacgcgc
    14461 gtcatccggg tcgttcggag cacacaggat tggctcttta atatccgcca gatcgatgtc
    14521 aatcactgcc gcgtattccg catctgcatc ggcttccagc agctcaggat tcgccagcca
    14581 tttttccatg ccctgaatac gacgttccag ggtacgacga tcgccgtaac cttccgcgat
    14641 catccacttc agcaggacga tattagagtt aaggtattcg atgattggtt ctttgttcag
    14701 cttgatagta caaccagcgg cagaacgctc agcggacgca tcggtaagct caaaggcctg
    14761 ctcaactttc agatccggca gaccttcaat ttccagaatg cggccagaga agatgttttt
    14821 cttgcctttc ttctcaacgg tcagcagacc ttgtttgatg gcatacagcg gaatggcgtg
    14881 taccagatcg cgcagggtga tgcccggctg cattttgcct ttgaagcgca ccagaacgga
    14941 ttccggcata tccagcggca ttacgcctgt tgcggctgca aatgccacca gaccagaacc
    15001 cgccgggaaa gagataccga tcgggaaacg ggtatgggag tcacctccgg taccgacggt
    15061 atccggcagc agcatacggt tcagccagga gtggattacg ccgtcacccg gacgcagcga
    15121 cacaccgcca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg
    15181 cttcggatac gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg agaagcccag
    15241 gcacgccagg tctttcagtt catcacgggt catcgggccg gtagtgtcct gtgaacctac
    15301 agaagtcatt ttcggttcgc agtacgcgcc cggacgaatg cctttcacgc cacaggcacg
    15361 gcctaccatt ttctgcgcca gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg
    15421 acggaacaca tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg tcaggccacg
    15481 cccgataatc agcgggatac ggccgccagc acgcacttca tcaatcagca cgtcggtttt
    15541 cagttcgaag gtcgccagca gttcgcctgt ttcgtggtta cgcacttcac ctttgtacgg
    15601 gtaaacgtca atcacgtcgc ccatgttcag gttagagacg tcgacttcga ttggcagtgc
    15661 acccgcgtct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga ggcacaaacc
    15721 accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca gaacggagtt
    15781 cgtggcggat ttacgcgatg aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa
    15841 acctttctgt tgcagagctt caatctgttt gatcgggcca acaacacctg gctggtctgg
    15901 ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga tatccgggcg
    15961 cgaccacgca tccggtgccg gagaaaggtc atcggtgttg gtttcgccag tgactttgaa
    16021 gacggtaacg gtcagttttt cagccagcgc cgggcgattc ataaaccatt cggcatccgc
    16081 ccaggactgc attacctgct tcgcatattc gttgcctgct ttcgctttct cttctacgtc
    16141 atagaagtta tcgaacatca gcagtgtgtg agaaagtgct ttggcagcga tcggtgccag
    16201 tttggcatca tccagcgcgt cgatcagcgg atgaatgttg taaccaccct gcatggtgcc
    16261 cagcagttcg atggcttttt ccggagtcag cagaggggat ttggcttcgc ctttcgcgat
    16321 agcagccagt aagcctgctt tgacataggc ggcttcatcg acgccagggg gaacacggtt
    16381 ggttaacaga tctaacagga attcttcttc gcccgcgggc gggtttttca gcagctctac
    16441 aagtgcggcc atttggtttg catccagggg tttgggcgca atcccctcag cggcacgctc
    16501 agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca ttgtcatagt
    16561 gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta aatgcccagt
    16621 accgcaacgg gcagaatagc aggattttga cgacgtgtta atctgtttac aaaaaagcaa
    16681 cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc cgtaaaaact
    16741 tcgttaattc gcatggtgat aatcaccttt ctcaacgaat cccaagatta gtcaaaattt
    16801 aaactaccgc ctctttatac tcggattaac agcacctgcg ggtgacagtt cgccgaccat
    16861 tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc ccatgtgctg
    16921 gcactaattt gcagtgccgg gctttgtgcc gcctctgccg ggctatatat aaaaagccgc
    16981 acagtggaag cggctgtgga aacgcaatcg acacaactgg ctgtgtctga cgctgccgcg
    17041 gttacgcttc ctgcaacggt ttccgcgcct cccgtaacac ccgccgtcgt taaatccgca
    17101 ttcagcactg cacaaataga tcaatgggtc gcgcccgtcg cgctgtatcc cgacgcccta
    17161 ctttcgcagg tgctgatggc atcaacctat ccggcaaacg ttgctcaagc agtgcaatgg
    17221 tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc tgaccagccg
    17281 tgggacgcca gcgttaaatc actggtggcc tttccacaat tgatggcatt gatgggcgaa
    17341 aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca ggacgtgatg
    17401 gactcggtac agcgattgcg gcaactggcg caacaaaccg gttcgctgaa gtcatcaaac
    17461 gaacaaaaag ttattaccac aacgaagaaa gctgtaccgg taaaacagac agtcacggca
    17521 cccgtcatac catccaatac cgttttaact gccagccccg tcattacaga gcctgcaaca
    17581 accgtcattt ccattgagcc cgccaatcct gatgtggtct atattcccaa ctacaaccca
    17641 accgtggttt acgggaactg ggccaatact gcgtatccgc cggtttatct gccaccacca
    17701 gccggagaac cgtttattga cagctttgtg cgcggattcg gctataggat gggtgttgct
    17761 accacgtacg cactattcag cagcatcgac tgggatgacg acgatcatga ccatcatcat
    17821 catgacgatg atgattatca tcaccacgat ggcggtcatc gtgacggtaa tggctggcaa
    17881 cacaacggcg acaacatcaa tatcgacgtc aacaatttca accgtatcac cggtgagcat
    17941 cttactgata agaatatggc atggcggcac aatccaaact accgtaatgg tgtgccctat
    18001 catgatcagg atatggcaaa gcggtttcat caaaccgatg tcaacggcgg aatgagtgcc
    18061 acgcagctac ctgctccaac acgcgacagc cagcgtcagg cggcagcaag tcagtttcag
    18121 caacgaacac acgccgcccc cgtcattaca cgagataccc aacgtcaggc agcggcacag
    18181 cggtttaatg aagctgaaca ctatgggagc tatgacgact tccgcgactt cagccgtcgc
    18241 caacccctga cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc agcttctcct
    18301 gagcagcgcc aggcagttcg cgagagaatg cagactaacc cgcagatcca gcagcgaaga
    18361 gaggcagcgc gtgagcgcat tcagcccgcc tcgcctgagc agcgccaggc agtccgcgag
    18421 aaaatgcaga ctaacccaca gatccagcag cgaagagacg cagcgcgtga gcgtattcag
    18481 tcagcctcgc ctgagcagcg ccaggtgttt aaggaaaaag tacagcagcg cccactgaac
    18541 caacagcaac gtgataacgc ccgccagcgt gttcaatcag catcacctga acaacgtcag
    18601 gtttttcggg agagagttca ggagagccgc ccacaacgtc taaacgacag taaccgtact
    18661 gccagattga ataacgatca acggtcagca gtacgcgaac gtctctctga gcgcggagca
    18721 aggcgactgg aaaggtaaat tacagacgta aaaaaagcgg cgtggttagc cgctttttta
    18781 attgccggat gttccggcaa acgaaaaatt acttcttctt cgctttcggg ttcggcaggt
    18841 cggtaatgct accttcgaac acttctgccg ccaggcccac agactcgtgc agagtcgggt
    18901 gcgcatggat ggtcagcgcg atgtcttcag cgtcacaacc catttcgatt gccaggccga
    18961 tttcacccag cagctcgccg ccgttggtac cgacaatcgc accaccgatc acacggtgag
    19021 attctttgtc gaaaatcagc ttggtcatac cgtctgcgca gtcggaagcg atagcacgac
    19081 cagaagcagc ccacgggaag gtggcggttt catagctgat gcctttctct ttcgcttctt
    19141 tctcagtcag acctacccat gcaacttctg gttcggtata ggcgatagac gggataactt
    19201 tcggatcgaa gtagtgtttt ttaccggcga taacttcagc ggcaacgtga ccttcgtgaa
    19261 cacctttgtg tgccagcatc ggctgaccaa cgatatcgcc gatagcaaag atgtgcggta
    19321 cgttggtacg cagctgtttg tcaacgcgga tgaaaccacg gtcgtccact tccacgccag
    19381 ctttgcctgc gtcgaggttt ttaccgttcg gcacacgacc aatcgctacc agcacggcgt
    19441 cgtaacgctg cggttcagcg ggtgcttttt tgccttccat cgtcacataa ataccgtctt
    19501 ctttcgcttc aacggcggta actttggttt ccagcatcag gttgaatttc ttgctgatac
    19561 gcttggtgaa gactttaacg atgtctttgt cagctgccgg gataacctgg tcgaacattt
    19621 caaccacgtc aatctgtgaa cccagcgcgt ggtatacggt gcccatttcc agaccgatga
    19681 taccgccacc cattaccagc aggcgttctg gtacttcttt cagttccagc gcgtcagtgg
    19741 agtcccagat acgcggatct tcatgcggaa taaacggcag ttggatcggg cgagaacccg
    19801 ctgcaatgat cgcgttgtcg aagttgatca cggttttacc gttctcacct tcaacttcca
    19861 gggtgttagc cccggtaaat ttacccagac cgttgaccac tttgactttg cggcctttcg
    19921 ccataccagc cagaccaccg gtcagctgat tgatcacttt ctctttccag gtacgaatct
    19981 tgtcaatatc ggttttcggt tcgccgaaga cgataccgtg ttcagccagc gctttggctt
    20041 cttcgataac ttttgctacg tgcagcagtg ctttagaagg gatacagccg acgttcaggc
    20101 aaacaccgcc aagggtgttg taacgttcta cgattacggt ttccagacct aaatcagcgc
    20161 aacggaaggc agcggagtaa cctgcggggc ctgccccaag taccacgacc tgagttttga
    20221 tttcagtact catcatgacc tctatatatt tatctccggc ggtcataccc gtcgtctttc
    20281 aggccgcagc ggcgttagct tcacgccctc accccagtca cttacttatg taagcccctg
    20341 gggattctgg cgcttgctac cttgctgcac ccagaaatcc atagggtatg tgaagtaata
    20401 ccctaaccac caccgggtcg ttctatccgt cggtatttta caaaattgtt aacaattttt
    20461 aaacaacaaa cggcaaccga tttgtctatt cgctaataac ctcaatacat tcatgagatt
    20521 accagaaaaa agccggccgt tgggccggct cttttactta catcaccaga cggcgaatgt
    20581 cagacagcgt gttgttaatg atggtaatga aacgggcacc atcagcaccg tcgatcacgc
    20641 ggtggtcgaa ggagagagaa atcggcagca tcagacgcgg cacgaactct ttaccattcc
    20701 acaccggctc catcgcggac ttggaaacgc cgaggatagc cacttccggc gcgttcacaa
    20761 tcggcgcgaa gtgggtagta cccaggccgc cgatgctgga gatggtgaag caaccgccct
    20821 gcatttcgcc cgcagtcagc ttaccgtcac gcgctttctt agaaatagtc atcagctcgc
    20881 gagacagctc gatgatgcct ttcttgttga cgtctttgaa taccggaaca accagaccgt
    20941 tcggggtatc caccgccaca ccgatgttga tgtatttctt cagggtcaga cgctgaccgt
    21001 cttccgacag cgaactattg aagcgaggca tctgctcaag agctgcagca acggctttca
    21061 tgatgaagac aaccggggtg atcttcacat ccagcttacg tttcgccgct tcttcgttct
    21121 gctgtttacg gaacgcttcc agctcggtga tatcggtttt gtcgaagtgg gtaacatgcg
    21181 ggatcatcac ccagttacgg ctcaggttag caccagaaat tttctggata cggcccagtt
    21241 ccacttcttc gatttcacca aacttgctga agtccacctt cggccacggc agcatgcctg
    21301 ggataccgcc gccagtcgcc gccggagctg cttctgcacg tttgatagct tctttcacgt
    21361 aagcctgaac gtcttcgcgc aggatacgac ctttacggcc agtgcccttc actttcgcca
    21421 ggttaacacc aaactcgcgt gccagacggc ggatcagcgg agtcgcgtga acataagcgt
    21481 cgttttcagc aaattcagat ttgccttccg ctttcgcagc tggtgctgct gccggggctt
    21541 cagcttttgc tgccggtgcc ggcgctgccg cttcctgttt cgcaggagct gccgcaggcg
    21601 ctgcgccttc aacttcgaag atcataatca gcgagccagt tttcacttta tcgccaacgt
    21661 tgactttcag ttccttcacg acgcctgcaa acggcgccgg aacttccata gaagctttgt
    21721 cgccttctac ggtgatcagt gactgttcag cggcaacttt gtcgcccact ttcaccatca
    21781 cttcagtcac ttcaacttcg tcaccgccga tatccggaac gttaacttct ttcacgccag
    21841 ctgctggtgc aggggccgct gccggagccg cttcctgttt agccgccgga gctgccgcgc
    21901 ctgcttcacc cgcgacttcg aagaccataa tcagcgagcc ggtagacact ttgtcaccca
    21961 cgttcacttt gatctctttc acggtgccag caaacggagc cggaacttcc atagaagctt
    22021 tatcgccttc tacggtgatc agcgactgtt cagcttcaac tttatcgcca actttcacca
    22081 ggatttcggt cacttcaact tcgtcgctgc cgatatccgg aacgttaacg tcttttgccg
    22141 ccgcagccgc gggtgctgct gccggagctg cttctttctt ctcttctgcc tgagcaggtg
    22201 cagcgtctgc tgcaccgtcg gcggaatcga aaatcataat cagtgcgccg gtctgggttt
    22261 tatcgccaac agagactttg atctctttaa cgatacccgc ctgcggagac ggaacttcca
    22321 tagaggcttt gtcgccttct acggtgatca gcgactgttc ggcttcaact ttgtcgccca
    22381 ctttgaccag gatctcggtg atttcaactt catcagcccc gatgtccggt actttgattt
    22441 cgatagccat tattctttta cctcttacgc cagacgcggg ttaactttat ctgcatcgat
    22501 gttgaatttg gcgattgcgt cagcaaccac tttcttatcg atttcgccac gtttagccag
    22561 ttcgcccagc gccgcaacca cgacataaga agcatcaact tcgaagtggt gacgcaggtt
    22621 ctcacggctg tcggaacgac cgaagccatc agtacccagt acgcggtagt cgtcagccgg
    22681 tacgtaagta cggacctgct cagcgaacag tttcatatag tcggtagatg ccactgccgg
    22741 agcgtcgttc atcacctgag cgatatacgg aacgcgcgga gtttccagcg gatgaagcat
    22801 gttccagcgt tcacaatcct gaccatcacg cgccagctcg gtgaaggagg tcacgctata
    22861 aacgtcagaa cctacgccgt aatctttcgc caggatctct gccgcttcac ggacgtgacg
    22921 caggatagaa ccggagccca gcagctgaac tttacctttg ctaccttcaa tggtttcgag
    22981 tttgtagata cctttacgga taccttcctc agcaccttcc ggcattgccg gcatgtggta
    23041 gttttcgttc agcgtggtga tgtagtagta aacgttctct tgtttttcac cgtacatacg
    23101 ctccagaccg tcatgcataa tgacagcaac ttcgtaagcg taagccgggt cgtaagagat
    23161 acagttcgga atagtcagcg actgaatgtg gctgtgacca tcttcatgct gcagaccttc
    23221 tccgttcagg gtggtacgac cggaagtacc gccgatcagg aagccacgcg cttgctggtc
    23281 gccagccgcc cagcacagat cgccaatacg ctggaagccg aacatcgagt aatagatgta
    23341 gaacgggatc atcggcagat tgttggtgct gtaagaggtc gccgctgcca gccaggaaca
    23401 acctgcgccc agctcgttga tcccttcctg cagaatctga cctttctcgt cttctttata
    23461 gtaagcaacc tgctcgcggt cctgcggggt gtactgctga ccgttcgggc tgtaaatacc
    23521 aatctgacgg aacagacctt ccataccgaa agtacgcgct tcgtcggcga tgatcggtac
    23581 cagacgatct ttgatcgact tgttcttcag cattacgttc agggcacgaa cgaaagcgat
    23641 agtggtagag atctctttgc tctgctcttc cagcagcgcg ccgaagtctt gcaggctcgg
    23701 cagctcaagc ttctcggtga agttcggctg acggcttggc agataaccgt gcagtttctg
    23761 acgctgtgcg tgcagatagg tatgctcttc agaaccttcc gggaaggtga tgtacggcag
    23821 tttttcgata tctgcatcag acaccggcac attgaaacgg tcgcggatgt gacgcacgcc
    23881 gtccatgttc attttcttaa cctggtgcgc gatgttttta ccttcagccg cgtcgcccat
    23941 gccgtaacct ttaatggaat gagcaaggat tactgtcgct ttgcctttgg tttcctgcgc
    24001 tttcttgaat gcagcgtaga ttttcttcgg atcgtgacca ccacggttca gtgcccagat
    24061 ctgctcgtca gtccagtctg caaccagtgc tgcggtttca ggatatttac cgaagaagtg
    24121 ttcacgaacg tacgcaccat ctttcgattt gaaggtctgg tagtcgccgt caacggtttc
    24181 gttcatcagc tggatcagtt taccgctggt atctttacgc agcagttcat cccaacggct
    24241 accccacatc actttgatca cgttccagcc agcaccttcg aagatgcctt ccagttcgtt
    24301 gatgatcttg ccgttaccgg tgaccgggcc gtcaagacgc tgcaggttac agttgataac
    24361 gaagaccagg ttatccagtt tttcacgggt agcgatggtg atcgcacctt tggattccgg
    24421 ttcgtccatt tcaccgtcgc cgaggaacgc gtaaacggtc tgtttagagg tatctttcag
    24481 gccacggtgt tccagatatt tcaggaattt agcctggtaa atagcaccaa tcggccccag
    24541 ccccatagat acggtcggga actgccagaa ttccggcatc agtttcgggt gcggatagga
    24601 agagaggcca ttgccgtgaa cttcctgacg gaagttatcc agctgctcct gagtcagacg
    24661 accttccagg aaagcacgag cgtacacgcc cggggagatg tggccctgga agtaaaccag
    24721 gtcgccgcca tcctgctcgt tgcgtgcacg gaagaagtgg ttaaagcaca catcataaat
    24781 ggttgcggaa gactggaagg acgccatatg gccgcccagt tcgaggtctt ttttcgacgc
    24841 acgcagcacc gtcatgatgg cgttccagcg gatagctgaa cgaatacggc gttccagttc
    24901 cagattaccc ggatactccg gttgttcttc aacggggatg gtgttgatgt agttgctgat
    24961 acctgtgcct gcggctacgt ttacaccgcc tttgcgggct tcagcaagca gttggtcgat
    25021 cagatactga gcacgctcaa caccttcttc acggatgacc gattcgatcg cctggagcca
    25081 gtcgcgagtt tcgatcggat ccacgtcatt tgggaaacgt tctgacatgg gttattcctt
    25141 atctatctaa taacgttgag ttttctggaa cctgtcccat tgaactctcg ccggaaagct
    25201 caataagaca ggttctacgt ttagttgccg cgcttttata tgcgcttgat ttacaacatc
    25261 ttctggataa tttttaccag taaaatcact aattctttcg ttgctccaga cgacgcagag
    25321 aacgctcacg gcggctctct tcacgacttc tgtcgagcaa aatttcttcg ataaaggcca
    25381 gatggcgatg cgatgcttcg cgcgcttctt ccggcttacc ggccataatc gcttcaaata
    25441 tgcgggtgcg gtgactactc accagcggca gcatctcgcg acgcgaatag agcaattcga
    25501 agttctggcg gacattctgg gccaacatcg gctccataca ccttagcaga tgaagcagaa
    25561 ccacattgtg ggccgcttca gtgacagcaa tctgatactg gagtacggcg tttgattccg
    25621 cgtccagatc gccagactgc tgcgccagtt ctatggcgtg atggagttca cggatgcgtt
    25681 ccttgtcttc atcggtacta cgcagcgcgg cgtaataagc ggcgatacct tccagggcgt
    25741 gtcgtgtttc gagcaagtca tactgtgact caggatggtc ggagagcagc tccaccagcg
    25801 gatcgctgaa gctttgccat aggctgctct ggacaaaagt gccgccaccc tggcgacgaa
    25861 gcaacaagcc cttcgcttcg agacgttgaa tcgcctcacg caaggaggga cgggagacgt
    25921 caaactgttt tgccagttcg cgttccggtg ggagtttttc gcccgggcgg agagtgcctt
    25981 cgaggatcaa aaactccagt tgctgctcaa tcacatcgga gagttttggt tggcggattt
    26041 tgctgtaggc catgagttcc tgtcttaagc cacttgccga agtcaattgg tcttaccaat
    26101 ttcatgtctg tgacgctaaa gtaacaaagt attcacctta tgtccataca ggttttgatt
    26161 gaaatcatga aactgtgcac attttaacaa cttgacatat ataacgtttc aaagttgtaa
    26221 ctatgcacaa atgtagactt tacgtaggaa agggattttt taacgattat taaactataa
    26281 aaatccgaat tgaaccgatt cacttaccaa ttttgtgatt tttaattcaa ttaaaacgaa
    26341 tttaaattca ttctacatat tgagaggggt tgaggctgag ctttacaaac ggtttctttt
    26401 taagcaactc atcttcaacc atgcataaag cgggtgcatt cgctgccgca taccattatt
    26461 cttgatctga cggaagtctt tttgtaacaa ttcaaacttc tttgatgtaa acaaattaat
    26521 acaacaaacg gaattgcaaa cttacacacg catcactgcg tagatcaaaa aaacaaccac
    26581 cgcacgaggt ttcatgatgg aaggtcaaca gcacggcgag cagctaaagc gcggccttaa
    26641 aaaccgccat attcagctta tcgcgctggg tggcgcgata gggaccgggt tattcctggg
    26701 tagcgcctcc gtaatacagt ccgcagggcc agggattatc ctgggttacg ccattgctgg
    26761 ttttatcgcc tttctgatca tgcgtcagct gggtgaaatg gtggtcgaag aacctgtcgc
    26821 aggctccttt agccactttg cttataaata ctggggcagt tttgccggtt tcgcctctgg
    26881 ctggaactac tgggtactgt acgttttagt tgccatggct gagctgactg ccgtgggtaa
    26941 atacattcag ttctggtatc cggaaatccc cacctgggtt tctgccgccg tattctttgt
    27001 ggtgattaac gccatcaacc tgaccaacgt taaagtgttt ggcgagatgg agttctggtt
    27061 tgccattatc aaagttatcg ctgtggtagc gatgatcatc ttcggcggct ggctgctatt
    27121 cagtggcaac agcggcccgc aggcgaccgt tagcaacctg tgggatcagg gtggtttcct
    27181 gccgcacggc ttcaccgggc tggtgatgat gatggcgatt atcatgttct cgttcggtgg
    27241 tctggaactg gtggggatca ccgcagcaga agctgataac ccggagcaaa gtataccgaa
    27301 agcaactaac caggttatct accgcatcct gattttctat attggttcgt tagccgttct
    27361 gctctcactg atgccgtgga cccgcgttac cgccgatacc agtccgtttg tgctgatctt
    27421 ccacgagtta ggcgatacct ttgtggcgaa tgcgctgaac atcgtggtac tgactgcggc
    27481 gctctccgtg tacaacagct gcgtatattg caacagccgt atgctgtttg gtctggcaca
    27541 acagggtaat gcgccaaaag cgctggcgtc tgtcgataaa cgtggtgtac cagtaaatac
    27601 cattctggtg tctgcactgg taacggcgtt gtgcgtactg attaactacc ttgccccaga
    27661 gtccgctttc ggactgttaa tggcgctggt ggtatctgca ctggtaatca actgggcgat
    27721 gattagcctg gcgcatatga aattccgtcg cgccaagcag gaacaaggcg tggtaactcg
    27781 cttccctgct ctgctttatc cgctgggtaa ctggatctgc ctgctgttta tggcggcggt
    27841 actggtgatt atgctgatga ccccaggaat ggcgatttcg gtatacctga tcccggtatg
    27901 gctgatcgtg ttaggtatcg gctatctgtt taaagagaaa accgccaaag ccgtaaaagc
    27961 gcattaatct ctctacgccc tcacccgtac agggtgaggg caataatctt tacaccaacg
    28021 ccccgtaaat cgtcagtagt gcaatcacca ccacgaccac gaacgaggtt ttcttcgcca
    28081 ttgaaaccgc tgccttcggc gtctccacct tatcgacatg cggttcacgc gccagagaga
    28141 actgcgccag acgcgttaac acctgatact gcgaagtatg gaagtcaccc agcgaagcaa
    28201 accaggccgg taacgctttc tccccatgac cgatcaaggc atataccaca cccgcaagac
    28261 gaaccggcac ccaatccagt acatgaagca cggcatcaat gccggactgt aaacgatgat
    28321 gcggcgtctg gtatcgcgcc agccagtatt gccatgcacg caaaaacgca taccccatca
    28381 gcgtaacggg tccccagatt ccccccacaa tcaaccagaa cagcggagca agataaaaac
    28441 gaaagttaat ccacagcaat gcattttgca gctcacgcaa atactcacgt tcatcgcagc
    28501 ctgccgggac gccgtgaatc atggtgagtt cgccagccat cgtgacacgg gcatggctat
    28561 cattacgtga agcagctgtc agataagcat gataatgaag acgaacttta cctgcgccaa
    28621 tacacagcaa accaatcagc agccacacca gcagcgtggg aacgttgaac aatactccct
    28681 gcaatgcgcg taacagtaaa aaagtcacgc ccatcgcaat aatggtcatg cctaacgtgc
    28741 gcccgagaga aaaatgtttc acccgccgaa agaacgcttc aagacgatga tcaagctgcc
    28801 agtgctcgcc caacttaaac aggcgctcga aaattaacac cagtaaggtt gtaaatagcg
    28861 tcatgttgtc tccttgctga ccagcgcacg aaaccgcgcc caatcaaatg cagggccggg
    28921 atcggttttc cgatccggcg caatatcaca atggcccgtc atgttattag cgatatctgg
    28981 atagcgatca atcagtgccc gcgtaaccgc cgcaagctgt tgatactgcg catcggtata
    29041 cgccagcgta tcggtgcctt caagctcaat cccaatagaa aaatcattgc agcgttcgcg
    29101 cccctgatac tgagagactc ccgcatgcca tgcacgttta tcgaaaggaa catactggac
    29161 tatttcacca tcacggcgaa tcaaacagtg agcggagacg cgcaaatggg cgatctcagc
    29221 aaagaaagga tgtgcctgcg gatcaatagt tccagtgaat aatgcgtcga tccacggacc
    29281 gccaaactcg cctggcggca ggctaatatt gtgcaccacc agcagggtgg gtgtttcgtc
    29341 atccgggcgg caatcgtaat gtggtgaggg aacgcggcgc gcgccaacca gccacccctg
    29401 ttctaacaac atgcaggatc tccttatatg tggtgctaat acccggttca gagtagcatg
    29461 tttctacctt atgattcgtt agctatctgg agttttaaca tgccgcctcg ccgctataac
    29521 cctgacaccc gacgtgacga gctgctggaa cgcattaatc tcgatatccc cggcgcggtg
    29581 gcccaggcgc tgcgggaaga tttaggcgga acagtcgatg ccaacaatga cattacggca
    29641 aaacttttac cggaaaactc tcgctctcat gccacggtga tcacccgcga gaatggcgta
    29701 ttttgcggca aacgctgggt tgaagaggtg tttattcaac tggcaggcga cgatgtcacc
    29761 ataatctggc atgtggatga cggcgatgtc atcaacgcca atcaaccctt gttcgaactt
    29821 gaaggcccat cccgcgtgct gttaacgggc gaacgcactg cgcttaattt tgtgcaaacc
    29881 ctttcaggag ttgccagtaa ggtacgccac tatgtcgaat tactggaagg caccaacacg
    29941 cagttgttgg atacgcgcaa aaccttacct ggcctgcgtt cagctctgaa atacgcggta
    30001 ctttgcggcg gcggagcgaa tcaccgtctg gggctttctg atgccttcct gatcaaagaa
    30061 aatcatatta ttgcctccgg ctcagtgcgc caggccgtcg aaaaagcgtc ctggctgcac
    30121 ccggatgcgc cagtagaagt cgaagtagag aatctggaag aacttgatga agccctgaaa
    30181 gcaggagccg atatcatcat gctggataac ttcgaaacag aacagatgcg cgaagccgtc
    30241 aaacgcacca acggcaaggc gctactggaa gtgtctggca acgtcactga caaaacactg
    30301 cgcgaatttg ccgaaacggg cgtggacttt atctccgtcg gtgcgctaac taaacacgta
    30361 caagcactcg acctttcaat gcgttttcgc taattgctga cagatttatc cgctccatcg
    30421 cgggcggata atctcccctt ccccaacttt cttcgtaacg cctcgcaaat ttcttgtctt
    30481 tcgctgcaac gaaataaaaa acctcggaag ctgctttccc gtttcgtttt ttgcccctcg
    30541 cttgtgctga tggcgtttgc caaagtagcg ccaaccaaat caaggagcga aacagatgga
    30601 caagcaacgc ggttttacac ttatcgaact gatggtggtt attggcatca ttgccatttt
    30661 aagcgccatt ggtattcccg cttatcaaaa ctacctgcgc aaagctgcac tcaccgacat
    30721 gctacaaacc tttgtgcctt accgtaccgc cgtagagttg tgcgcgctgg aacatggtgg
    30781 a
//
LOCUS       DAJTNN010000029        29722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTNN010000029 DAJTNN010000000
VERSION     DAJTNN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.029"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..706)
                     /locus_tag="OO150_001174"
                     /pseudo
     CDS             complement(1..706)
                     /locus_tag="OO150_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(681..1481)
                     /gene="otsB"
                     /locus_tag="OO150_001175"
                     /pseudo
     CDS             complement(681..1481)
                     /gene="otsB"
                     /locus_tag="OO150_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(1648..2634)
                     /gene="araH"
                     /locus_tag="OO150_001176"
     CDS             complement(1648..2634)
                     /gene="araH"
                     /locus_tag="OO150_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5515392.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2649..4163)
                     /gene="araG"
                     /locus_tag="OO150_001177"
     CDS             complement(2649..4163)
                     /gene="araG"
                     /locus_tag="OO150_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5515393.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGAIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4233..5221)
                     /gene="araF"
                     /locus_tag="OO150_001178"
                     /pseudo
     CDS             complement(4233..5221)
                     /gene="araF"
                     /locus_tag="OO150_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5935..6438
                     /locus_tag="OO150_001179"
     CDS             5935..6438
                     /locus_tag="OO150_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5515394.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6517..6768)
                     /locus_tag="OO150_001180"
     CDS             complement(6517..6768)
                     /locus_tag="OO150_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5515395.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6883..6969)
                     /gene="azuC"
                     /locus_tag="OO150_001181"
     CDS             complement(6883..6969)
                     /gene="azuC"
                     /locus_tag="OO150_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5515396.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7232..7555
                     /locus_tag="OO150_001182"
     CDS             7232..7555
                     /locus_tag="OO150_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5515397.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7726..8223
                     /gene="ftnA"
                     /locus_tag="OO150_001183"
     CDS             7726..8223
                     /gene="ftnA"
                     /locus_tag="OO150_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5515398.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8261..8500)
                     /locus_tag="OO150_001184"
     CDS             complement(8261..8500)
                     /locus_tag="OO150_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5515399.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            8691..9902
                     /gene="tyrP"
                     /locus_tag="OO150_001185"
     CDS             8691..9902
                     /gene="tyrP"
                     /locus_tag="OO150_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5515400.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVR"
     gene            complement(9964..10629)
                     /gene="yecA"
                     /locus_tag="OO150_001186"
     CDS             complement(9964..10629)
                     /gene="yecA"
                     /locus_tag="OO150_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5515401.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10825..10911)
                     /locus_tag="OO150_001187"
     tRNA            complement(10825..10911)
                     /locus_tag="OO150_001187"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10875..10877),aa:Leu,seq:taa)
     gene            complement(10924..10997)
                     /locus_tag="OO150_001188"
     tRNA            complement(10924..10997)
                     /locus_tag="OO150_001188"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10963..10965),aa:Cys,seq:gca)
     gene            complement(11052..11127)
                     /locus_tag="OO150_001189"
     tRNA            complement(11052..11127)
                     /locus_tag="OO150_001189"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11092..11094),aa:Gly,seq:gcc)
     gene            complement(11279..11827)
                     /gene="pgsA"
                     /locus_tag="OO150_001190"
     CDS             complement(11279..11827)
                     /gene="pgsA"
                     /locus_tag="OO150_001190"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5515402.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCGAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11884..13716)
                     /gene="uvrC"
                     /locus_tag="OO150_001191"
     CDS             complement(11884..13716)
                     /gene="uvrC"
                     /locus_tag="OO150_001191"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5515403.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEDEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13713..14369)
                     /gene="uvrY"
                     /locus_tag="OO150_001192"
     CDS             complement(13713..14369)
                     /gene="uvrY"
                     /locus_tag="OO150_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5515404.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14664..14840
                     /locus_tag="OO150_001193"
     CDS             14664..14840
                     /locus_tag="OO150_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515405.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            14827..15051
                     /locus_tag="OO150_001194"
     CDS             14827..15051
                     /locus_tag="OO150_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5515406.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(15118..15840)
                     /gene="sdiA"
                     /locus_tag="OO150_001195"
     CDS             complement(15118..15840)
                     /gene="sdiA"
                     /locus_tag="OO150_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR5515407.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(16070..16822)
                     /gene="yecC"
                     /locus_tag="OO150_001196"
     CDS             complement(16070..16822)
                     /gene="yecC"
                     /locus_tag="OO150_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5515408.1"
                     /translation="MSAIEVKNLVKKFQGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(16819..17487)
                     /gene="tcyL"
                     /locus_tag="OO150_001197"
     CDS             complement(16819..17487)
                     /gene="tcyL"
                     /locus_tag="OO150_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5515409.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPILSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17502..18486)
                     /gene="dcyD"
                     /locus_tag="OO150_001198"
                     /pseudo
     CDS             complement(17502..18486)
                     /gene="dcyD"
                     /locus_tag="OO150_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
     gene            complement(18591..19391)
                     /gene="tcyJ"
                     /locus_tag="OO150_001199"
     CDS             complement(18591..19391)
                     /gene="tcyJ"
                     /locus_tag="OO150_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5515410.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19479..20030)
                     /gene="fliZ"
                     /locus_tag="OO150_001200"
     CDS             complement(19479..20030)
                     /gene="fliZ"
                     /locus_tag="OO150_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5515411.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQAHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(20076..20795)
                     /gene="fliA"
                     /locus_tag="OO150_001201"
     CDS             complement(20076..20795)
                     /gene="fliA"
                     /locus_tag="OO150_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR5515412.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLSTKLGKL"
     gene            complement(21115..22821)
                     /locus_tag="OO150_001202"
     CDS             complement(21115..22821)
                     /locus_tag="OO150_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5515413.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRILELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSAKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGAGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLL
                     QG"
     gene            23087..24493
                     /gene="fliD"
                     /locus_tag="OO150_001203"
     CDS             23087..24493
                     /gene="fliD"
                     /locus_tag="OO150_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5515414.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAKNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            24518..24928
                     /gene="fliS"
                     /locus_tag="OO150_001204"
     CDS             24518..24928
                     /gene="fliS"
                     /locus_tag="OO150_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5515415.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            24928..25294
                     /gene="fliT"
                     /locus_tag="OO150_001205"
                     /pseudo
     CDS             24928..25294
                     /gene="fliT"
                     /locus_tag="OO150_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            25372..26859
                     /gene="amyA"
                     /locus_tag="OO150_001206"
     CDS             25372..26859
                     /gene="amyA"
                     /locus_tag="OO150_001206"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5515416.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADVFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYNLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26893..27306)
                     /locus_tag="OO150_001207"
     CDS             complement(26893..27306)
                     /locus_tag="OO150_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5515417.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLALMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            27493..28698
                     /gene="yedE"
                     /locus_tag="OO150_001208"
     CDS             27493..28698
                     /gene="yedE"
                     /locus_tag="OO150_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5515418.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            28695..28928
                     /gene="yedF"
                     /locus_tag="OO150_001209"
     CDS             28695..28928
                     /gene="yedF"
                     /locus_tag="OO150_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5515419.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            29035..29706
                     /locus_tag="OO150_001210"
     CDS             29035..29706
                     /locus_tag="OO150_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR5515420.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
BASE COUNT         7391 a         7641 c         7118 g         7572 t
ORIGIN      
        1 ccggcagcct gtttggctat ttctttcggt tcaatgccga tcgggtagac ttctgttcga
       61 aatgctttgc cccaggctgt atggcttttt gcgctacgtg tcgtgacgcg ggtcaggtta
      121 gaaagacaat ccaggaacgc cagacgatcg ttttctgtct ggaaacccag caaatcataa
      181 tcacaaagct gttcaagcaa ggtgtcatat gtcggcagcg cgttgaagat ttccggtgtc
      241 gggaaaggaa tatgcagaaa gaaaccaatg cgattattca ccccacgttt gcgtaattca
      301 tgcgcaaagg gcaacaggtg ataatcgtgg atccagataa tgtcatcgtc ttgcaacagc
      361 ggcagtaatt tatctgccag caacgcattt acgcgtagat agccgtccca ggcaggacgc
      421 tgaaattgca tcagatcgag ccgataatga aaagcgggcc agagaacggc attggagaat
      481 tggttgtagt attcgtcaag gtcctgttcg ctgaggttaa aagaggccca cgtaatgtta
      541 ccttttttag cggctgatcc tcattccctg tttcaccact ccagccaaac cacagtccgc
      601 ctgtggcttt cagtgccccc agtatgccaa cggcaaggcc accggcactg gcggcgtgct
      661 cgtctggtgg tgcaatccgg ttagatacta cgactaaacg actcatagtc atcactcctg
      721 ttattttctc ttttttgttg taatgcggtg gttatcattt caagcctgct ccagacatcc
      781 ggcacacccg ccagtcgcca tgatgcctga gttgcacctg tgccaatttt tactgacatt
      841 ccgcccagtc ggttaacgac tgcgaagcca gattcatcgg ttaaatcatc gcccagaaat
      901 acgggcgttc gcccgataaa gggagcttcc tgcataaaag ctgcaattgc ctcaccttta
      961 ctggtacctc tcggtttgat ctcgacaaca cactttccct gctgtaacgc catttgtggc
     1021 cagatctgag taatacgtta cgctaatgtc attaatgcgt cttcatgctg cggagcctga
     1081 cgataatgca gcgcaaaagc catccctttc gcctccagct ccgcgccggg atactgagcg
     1141 ataactgtat gcagttgcac gctaatatca cgcgcaatcg catccggcag atgaacgata
     1201 tgcgttttac cattgatgtc acggcgctcc gccccatgca cgcccgctaa cgggaagcga
     1261 taaggttttg ccagtgcgtc aagctccacc attgagcgcc ctgatatcaa tgccaatgca
     1321 ccatcacttg cggttgccag tagctgtagt ccttgcagaa tattgtcagg cacgacgacc
     1381 tgatcgggat gcggtttgat ttccgccagc gttccatcga gatcaaaaaa ccaggcatat
     1441 ttcgcggata gttcaggggt ttcggttaac ggttctgtca cccggttctc ctacttctct
     1501 tttcttattg ttttgcgcag aacgcgcttt acagacatgt aagtatagac agtgtgacgg
     1561 gggtcgccat ttgcaacgaa gaacagccaa ccgttatggc ggttggctgt ggtgggaaaa
     1621 aacgctaaat tgttgcagaa aaaagcatca gacagtgcgt ttcgcttttt gcttgtaacg
     1681 gtcgaagatc accgctgcca gcaggattaa gccgcgaacc acgtactgcg cgaaaggaga
     1741 aatattaagc aggttcatgg cgttttccac ggtgcctaaa attaagatac ccgccaccac
     1801 atatgagatt tttccgatgc ctcctttcag agaaacgcca cctaaaacac aggcggagat
     1861 aacaatcagc tcgtaaccaa tcgacgtcat tggctgccca ctggtcatac gtgaagccag
     1921 aataattccg gctatcgcag ataccaggcc agagagaaca aagataataa ttttggtgcg
     1981 aacaaccggt acacccgcca gacgcgcagc ctcttcgttc ccgccaatcg ccagggtgtt
     2041 acgaccaaag gtggttttat tcagcaacaa accaaagata atcagacacg cgacggtgag
     2101 ccagattggc gcaggcagac cgaaccagtt agcgtaacca agggcaaaga agctttcatc
     2161 ttcgataccg accgctttac cgtctgaaat gatgtacgcc agacctcgaa caatctgcat
     2221 cgttgccagt gttgtgatca gagcatttat tttcagtttg gcgataacaa agccattgac
     2281 caggccacag agaatgccaa gcagcaaccc cgctgccacg ccaatccaca ggctttcagt
     2341 caggttgata accaccgccg tggtgacacc cgcacaggca attacggagg cgacagaaag
     2401 gtcaaagtca ccggaagcaa ggcagaacaa catgccacac gccaccatcc ccgacatgga
     2461 aattgccagg cccaaccctt tcatattaat gaaggtggca aaatttggga caaaaatggc
     2521 acaggcgata aagagcaccg caaacaccac cagcatgccg tactgatccc agatacgccc
     2581 gaagctgaat gacgacttag gtgcgccaga acccgatgta gaaacagaag acatcatact
     2641 ctccttactc aggcaactgc ctggctgact ttaggcatcg caaggctcag tgcctgacgc
     2701 tcatctgcct gctcgtgtaa caattcaccg gcgattgcac cttcccgcat caccacaatc
     2761 cggtcggcaa cgccgaggac ttcaggtaag tcgctggagg caaacagcac cgccacaccc
     2821 tgcgccgcca gcgcataaat tacgttatat atttcgtgct tagcaccaac atcaatgccg
     2881 cgcgtaggtt catccagcaa aatgaccttc atctcttccg ataaccagcg gcccagaatg
     2941 gctttttgct gatttccgcc tgagagattc atgatcagtt gctcagcgcc cggcgttttg
     3001 atgttgagcg aacgaatgtg ctgatcggca ttgttttctt cccaaccgtt gttgattaca
     3061 caaccgccga gcacatgttt gcgtctggca ctgatgttga tattgtcgcg aacggagtgc
     3121 acgggaataa tgccttccgc tttgcgatct tccgggcaga gcatcatgcc tgcggcaatg
     3181 gcgtggctcg gtttacgaat atcgatcggc tgttggtcga tataaacctg accggcggtg
     3241 atttgcgtcc cgccaaacaa gcctttcatt aattcgctac gccccgcccc taccagacca
     3301 aacagcccaa caatttcacc actgcgaacc gccagactta ttggcgtacg cacgcctggt
     3361 gctttcacag catcaagacg caggcgctcc tcgccataac tacgaggttg ccagccgtag
     3421 atatcgccaa tgtcgcgccc gaccatcgcc tgcaccagcg cgtcgtggtc aacctgctgc
     3481 atatcggtaa aggttttgac ataacgtcca tctttaaaga cagtgatggc atcgctgagg
     3541 gcaaatattt cttccatacg gtgagaaacg tataagatca cccgcccctc ttttcgcagt
     3601 tcacgaataa cgcggaaaag attgtcgatt tcgcgggcag aaagggagct ggttggctca
     3661 tcaaaggcga taattttagc gttacgcgcc agcgccttgg cgatttcaac catctgccac
     3721 tgaccaatgg agagatattt cagcggcgtg tccgggtcaa tatccatacc aagatgttta
     3781 agttgtaaac ccgcctcata attcagcaat gagcgattca caatgccgcc tttatgcggc
     3841 agctggccga gatagatgtt ttccgcgacg gtcatttccg gcacgagatg cagttcctgg
     3901 taaataatcg ccacacccgc gttaagtgct gcggtcgtgt cggaaaagga catttcctgc
     3961 ccattaatca ctacagaacc cgtggttggc gcatagttgc cgctgaggat ttttaagaga
     4021 gttgattttc ctgcgccatt ttcacccatc aacgcatgaa cctgaccggc atagcagtca
     4081 aaactaatat ccgtcagcgc cttaacgccg ggaaacgttt taccgatgcc gcgaaatgag
     4141 agatacgggg tagactgttg cataacgtct ccgtgaatca ctggtcgttc acaccccctc
     4201 tgcatcatgc agaggggaat ttttccggca aattacttac cgcctaaacc ttttttctcc
     4261 agttcttctt taaagttgtc acgcgtgatc agcaccacgt cggtaacttc ggtaaatttt
     4321 ggcggttcaa cgtcttttgc tacccagttg taaagcattt cgctggattt atagccatgt
     4381 acgtccgggc ttggcagcag ggaaccgtag aagccggttg cctgtacttt agacagttcg
     4441 ctcaccgcat ccacaccgtt aatgccaatg ccgataatat cggcagcttt aaagccctga
     4501 ccttccgtcg cgcgtacgcc gcccagcacg gtgctgtcgt tcataccgac gatcagccaa
     4561 tgtttaactt caggatgttg aaccagcatt gagttggcag cgtcaaatgc ccccgggatg
     4621 tcgttagatt tggtaggtac ctgataaatt tgtttttccg ggaatccggc cgctttcagc
     4681 gcatccatag atcccgtagt acggcggcgg gcagtatcca gttcgttggc ggtaatcacc
     4741 atcaccgcgc tttctttgac atcccagcca cgtttctgca tctctttata cagttcctga
     4801 ccctgacgtt cgccaatttt agtcgcgcca tcatcaccag cggaacggta tccattggct
     4861 tacctttggc gttaacaaac tgatcatcca cggcaatgac tttcatatcg tagccacgcg
     4921 ctttcgcgac gatggcagag ccgagtttgg ggtccggagt acaaataacg aaaccttttg
     4981 cgccactggc agccaggctg tcgatcgcgt tcaatgtttt ttcgccatcc ggcacggcaa
     5041 tcttaataac ctcaaaccct aaatccttcc cggctttatc ggcaaacttc cattcggtct
     5101 ggaaccacgg ctcttccggt tgcttcacca gaaaaccgag cttcaggttc tccgccatag
     5161 cggattgtga cataacggct gccagaccaa tggctgccag ggctttagta aatttgtgca
     5221 tggttctctc cagctttagt gtcgttttgt gtagggcaaa aacgaatgac attcgttaaa
     5281 ttaatcggaa aacaaagcat taccttttaa ctaaaagata agtgaccgtg ttgacatagt
     5341 tttagcgaga aattaattct ccataggaga gcaatatcac atcgcagaat tacagtgaga
     5401 acgtgcataa atttagcggg aaaagacata agggaaagcc aatttgtcag acaaattgtc
     5461 gaatgcacag cagattaatc cataagatta gcttggaaat ccttgttgtc tttggtaccc
     5521 atgcgggatg tcttcttttt aaccagtcaa taggccgcat tacctggcgt tgagtttttg
     5581 aaatggtgta ataaccgcaa ctcaaagatg tggaaaatgc acgtcattca tttcgccatt
     5641 aattatcact gtgctcatta attaacagaa cacgtataat gagagccatc tcgcaaaaat
     5701 gaaaaaacgt tttataaaat catcatttca tcaatgaaat gtaaaaatat ataaacttga
     5761 tgatttaagc attttcttat acccgttcag acgttattct tatttcagat catcgtcaga
     5821 attgactcca cgatcacatt tcggaccggc agaaaggaat tattctgcaa acagtaatta
     5881 tggtgttttg atttatcttg cacctctcca cttctggata taaggatatt aggtatggca
     5941 accgctggaa tgcttctcaa actcaactct caaatgaacc gcgagtttta cgcatccaat
     6001 ctctaccttc acctgagtaa ctggtgttct gaacagagtc tgaacggcac cgccactttc
     6061 cttcgcgccc aggcacagag taatgtgacc caaatgatgc gcatgtttaa ctttatgaag
     6121 agtgtcggcg ctacccccat cgttaaagcc attgatgttc ccggtgaaaa actgaactct
     6181 ctggaagaac tgttccaaaa aacgatggaa gaatacgagc aacgttctag tacgttggca
     6241 cagttagccg atgaagcgaa agaactgaat gatgattcaa ccgtcaattt cctgcgcgat
     6301 ctggaaaaag aacagcagca tgatggtctg ttgctgcaaa ccattcttga tgaagtgcgc
     6361 agtgcgaaac ttgctggtat gtgcccagtg cagaccgacc aacatgttct gaatgtcgtg
     6421 tcgcatcagc tgcattgatc atacatcggc gctaatgcat tgcgccgatg aagtttttga
     6481 gaaaccgctg cctcatcttt ttgaagcagc ggttttttaa tgggattcgc cttgtggggt
     6541 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6601 ggcggcggga tggttactaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6661 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6721 aaccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6781 atagcatttt gaaaaccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6841 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6901 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6961 cagtttcatc gcggcactcc gaccatttgt tatacatgtg ttattttctt tgtgcttata
     7021 atatgacaac catcacaaaa atcaatcttt atgtgatgca aatcacacaa ataccccctt
     7081 aatgttataa aaatgataat caaaaaacag cccccccatt tctgacacct acagatggca
     7141 agaaatagcg cctgccaggc gtcttttccg gtcattgtcg cagcgctgta acgcgtaaaa
     7201 tagtgctttc ttttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7261 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7321 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7381 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcaat
     7441 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7501 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7561 ttaatcagcg aggggatctt cgctgattaa aaaaatagcg gtaatgcgtt ttattcccat
     7621 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7681 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7741 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7801 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7861 cacgcccaag aagagatgac gcatatgcag catctgtttg attacctgac tgataccggc
     7921 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7981 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     8041 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcaatg gtatgtttct
     8101 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     8161 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8221 taatactctc cggcggcagg tttactgccg ccgtttcaaa tcagtgacgg caaatcttac
     8281 tctgatcggt cgaaaaaccg tctttcgaag gtataaattt gccttttgct gccagaaacg
     8341 ctaccagctc tcccgctgtc atcccttctg ccgagcaggt gtgaaaacgt gcctgttcac
     8401 caaaacgcgc tttaattgct gcttccagac tggcatgcgt atattgctcg cctgattcaa
     8461 tcatcatatt taacacttca tgaccgtgaa tagagtccat cgttcctcct caaaaaaagc
     8521 ctagcgtagc gattgccgct tatgaagact ttgcgccaga gcagaactga atgcttttta
     8581 ttgtacattt atatttacac catatgtaac gtcggtttga cgaagcagcc gttatgcctt
     8641 aacctgcgcc gcagatatca ctcataaaga tcgtcaggac agaagaaagc gtgaaaaaca
     8701 gaactctggg aagtgttttt atcgtggcgg gaaccacaat tggcgcaggc atgctggcaa
     8761 tgccgctggc tgcggcaggt gttggtttta gcgtcacgtt aatcttgttg attgggcttt
     8821 gggcgttgat gtgctacacg gcgctattac tgctggaggt gtaccagcat gttccggcag
     8881 ataccggtct gggcacgctg gcaaaacgct atctgggacg ctacggtcaa tggctgacgg
     8941 gcttcagtat gatgttctta atgtatgctc tgactgcggc atacatcagc ggtgccggtg
     9001 aattgttggc ctccagcatc agcgactgga caggtatttc aatgtcggca accgctggcg
     9061 tgctgttgtt cacttttgtt gccggtggcg tggtttgtgt tggaacatcg ctggtcgatt
     9121 tatttaaccg ttttctgttc agcgccaaaa ttatttttct ggtggtgatg ctggtattgc
     9181 tgctaccgca tattcacaaa gtgaatcttt taaccctgcc gttgcaacag gggctggctc
     9241 tgtcagcaat cccggtgatt tttacctcat ttggttttca cggtagcgtg ccgagtattg
     9301 tcagctatat ggatggcaac gttcgtaagc tacgcagggt gtttataacc ggtagtgcga
     9361 tccccctggt ggcatatatt ttctggcagg tggcaacgct tggcagcatt gattcaacaa
     9421 cctttatggg attgctggct aatcatgctg gattaaacgg gctgttacag gcgttacgcg
     9481 aaatggtggc ctctccgcat gttgagctgg cagtgcattt atttgctgat ttagccctcg
     9541 ccacgtcatt tctcggcgtt gcgttaggct tatttgatta tctggctgat ttatttcagc
     9601 gttcaaatac cgttggtgga cggttgcaaa ctggtgcaat tacctttctg ccgccgttgg
     9661 cgtttgcact gttttatcca cgaggatttg tgatggcgct gggttacgcc ggtgtggcgc
     9721 tggcggtact ggcattgatt atcccttcgc tgttgacctg gcaaagcaga aagcacaatc
     9781 ctcaggcggg ttaccgggtc aaaggtggtc gtccggcgct ggtggtggtg tttctctgtg
     9841 gtattgctgt gattggcgtg caatttttga ttgcggcagg gttgttacca gaagtgaggt
     9901 gatcagatag cctcaaattc cttattgggt gccagaatta acgctgacac ccaatttggc
     9961 ctcttaatgc aggcagcact gcttaaattt cttaccacta ccgcacgggc aaggatcgtt
    10021 acgccccggt ttctcttctg ctttgatcgg ttgctgaaca gctttttcct gcggatgcgc
    10081 catccagtac gcatgtagat caagcgccgc cagtcgaatg gcatctacgc tctcttcaaa
    10141 cgcttctggc gacatctttt ctacccgctc gaagttttcc tcagtaccgt gcagcgcaat
    10201 cgcctccagc gctggtttta acgaatcggg caacgttgac cagtcagaaa gtgccacgcc
    10261 ccgcatatag ccaaagcacc actcctcaac aatcgtcagc tcgctgccat caacttctcg
    10321 caagccgaat aacggctcaa actgctccgg gaattcgttc agacgctctg cggtatcggc
    10381 catatgttga aaagccaggt tcataaagcg cgtcatctct ttctctgacg cccagcgcgg
    10441 cacatagtca gccccacccc acacggcaac cagccactgt tccggttcaa tctcttgcgg
    10501 agaactcaac accgccgtca ataaatcgtc cagctccgcc acatcaagga tggcgtggtt
    10561 agtgttgtat ttggtcagaa tatcgtccag ccattccaac tcactttcgt ttaacggtcc
    10621 cgttttcata cgcttttcct tgtggatctc aactcgccag cacctatctt acatgccggt
    10681 ccgtatcaga gatacttttt gagtggcttt gccggtgatt aaaaattaag gagggtgtaa
    10741 cgacaagttg caggcacaaa aaaaccaccc gaaggtggtt tcacgacact gcttattgct
    10801 ttgattttat tcttatcttt cccatggtac ccggagcggg acttgaaccc gcacagcgcg
    10861 aacgccgagg gattttaaat cccttgtgtc taccgattcc accatccggg ctcgggaaga
    10921 aagtggaggc gcgttccgga gtcgaaccgg actagacgga tttgcaatcc gctacataac
    10981 cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca gacatccgct tgacgccgat
    11041 gtcttttaaa ctggagcggg aaacgagact cgaactcgcg accccgacct tggcaaggtc
    11101 gtgctctacc aactgagcta ttcccgcatt cattaagcaa tcagttaatc acttgatttt
    11161 attatcgtct ggcaatcagt gccgccgttc gatgcgttgc attctactta cctggcgcga
    11221 tgagtcaacg atatttttca ccacttttga tcgtttgctg aaaattacgc cgaaacgatc
    11281 actgatcaag caaatctgca cgcgcagcgc tcaaatattg caacattgac cacagagtca
    11341 gcaccgcagc cacaaagaaa agtgcaatac cggcgtactc aacccaaatg ttcggacgcc
    11401 acagcagcca ggccaacgcc accatctggg cagtggtttt cactttccca atccaggaga
    11461 cagccacgct actgcgttta cccaactccg ccatccattc gcgtagcgca gaaataataa
    11521 tttcacgggc gatcatcgtt gccgccggta gggtcaccca ccagctgtga taatgctcgg
    11581 ttaccagcac catggcgata gccacgagaa ctttatctgc tacagggtca aggaaagcac
    11641 caaaccgggt actctggttc cagcggcgtg ccagaaaacc atcgaaccag tcagtcaccg
    11701 ccgcgccgca gaaaatgagc gcggcggcaa acggcgacca ggtgacaggc agataaaaga
    11761 ccaatacaaa gaatgggata aggatgacac ggaacagtgt aagcaacgta gggatattaa
    11821 attgcataat gacgggtaac tatctgttgt cagtaagatt acccctatgt tgctacagag
    11881 acatcaatgt ttcaacgacc agaagatctt ttctgccaga ccttgcgaaa tacccggcac
    11941 ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc aaaccgccca tatatttcaa
    12001 caacatttgc cgacgttttg gcccgacgcc ttcaatggtt tccagggaac tggtattttt
    12061 gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga tcatgtgatt catcgcgaat
    12121 atgctggata acatgcagcg cgggtgaatc tggcggcaaa ctaaatccct catcttccgg
    12181 ctcaaagaac agcgtttcca gtccagcctt acgatctgct cctttggcaa cgccaagtag
    12241 cagcggatga tttttatccc atgagacatc cagttcggcg aagacatttt tcgcctgcgc
    12301 aagctggcct ttgccgccgt cgataaggat cacatccggg atcttactgt cgtcaatggc
    12361 tttaccataa cgccgacgca gcacctgatt catcgccgca taatcatcgc ccggcgtgat
    12421 gcctgtaatg ttatagcgcc gatactccgc acgcagcggg ccgttagcat caaacaccac
    12481 acaggaagcg acggtttgtt cgcccatggt atggctgatg tcaaagcact ccatccgctt
    12541 cacttccggc aatttcaaca cgctggcaag cgcggtcagt cgctggtgaa cggtagattg
    12601 ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg cgcgcgagtt tcagataacg
    12661 cgccctatcg ccgcgaggtt tggtttgaac attaatcttg cgtcccgcca gttctgaaag
    12721 ggaatcggcg agcagcgttt tatcgctaag attaaaatcg agcaggatct cacccggtaa
    12781 ggtgcgcatc tggctgcctt gtaaatagaa ctgacctacg aaggtttcta ccacctcgct
    12841 cagttccgta ccgccaggca ctttcgggaa atagctgcgg ctgccgagca ctttgccctg
    12901 acgaatgaac aatacgtgga cacaagccat gcccgcatcg aacgccacac caataacgtc
    12961 aaggtcgtcg ccggtattgg aaacaaactg tttttcggtg acgcgtcgca ccgcctgaat
    13021 ttggtcgcga atacgtgccg cttcttcaaa ctccagattc tgactggcag tttccatacg
    13081 gctaatgagt tgcgtaagca cctgatcatc tttgccagac aaaaacaggc gcacatactc
    13141 gacctgctga gcgtattctt cttcactcac cagtccttca acgcacggtc ccagacagcg
    13201 ccctatctga tattgcagac acggacgcga gcgattgcga taaacactat tttcgcactg
    13261 gcgaatgggg aaaatctttt gcagtagcgc cagagtttca cgtacggcat agccattcgg
    13321 gaacgggccg aaatattcac ctttggcatg cttcgcacca cgatgcatcg ccagacgcgg
    13381 gtgggtatcg ccactcagga agataaaagg ataagattta tcatcgcgta gcaaaacgtt
    13441 gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc aacagcgctt cggtttctgt
    13501 atgagtaacc gttacatcaa tttgctgaat ctgggcgacc agcgcttcgg ttttgcgcga
    13561 agcgaggttg ctacggaaat agctggaaag ccgttttttc aggtctttcg ctttgccgac
    13621 atagataacc gtaccaccag catcgtacat gcgataaacg cctggctggc tggttacggt
    13681 ttttaaaaac gcttttgcgt caaactgatc actcactgac ttgataatgt ctccgcatta
    13741 cacagaccat ggcgaattgc caggtgagtc agctcaacat cgccatgaat gtttagttta
    13801 ctgaacatac gatagcggta gctgttcacc gttttcggac tgagattgag ctgttctgag
    13861 atctcattga ccttctggcc cttggtgatc atcagcataa tctgcaattc acgttcagac
    13921 aaactggcaa atgggctttc tgttttttct ggttcgatct ggcttaacgc catttgttga
    13981 gcgatgtcag aagcaatgta acgctgccct gaatagacag aacgaatcgc actcacgact
    14041 tcctgcggag ccgcgccttt gctgaggtag cccgcagcac cggcctgcat gactttcgct
    14101 ggtaaagggt tttctgtatg aacggtaagc atgatgattt tgacatcagc tgtggaacgc
    14161 gcgattttac gcgtcgcctc aagaccgcca atgcccggca tactcatgtc cattagcacc
    14221 acgtcaacgg catttgtccg gcaccactta acggcgtctt caccgcacga tgcctcaccg
    14281 acgactttta taccctttat atcttccaga atgcgtcgta tccctgcgcg caccagttcg
    14341 tggtcatcaa caagtagaac gttgatcaaa gaaatatctc cagaaatagg gataacgcta
    14401 ctgatagtta gtcattcgta tattagcggt ttttattgca actttgaaac gttaaaaatg
    14461 gtgggtattc gatttttctc tcgtttttgt caattcgatt gtccacagtt atggtcacgc
    14521 ccgtcaccaa cggcttaccg gatcgtttca cagtaagccc tacgatgaaa tgtgacaaaa
    14581 attgacttta ttcagcaaaa atgaaaatca gcccgctgaa tatgtaacat taattaaccc
    14641 gaacaatacg ggcaaaaaca ttcatgataa aaatatttat tggtcattat attaacgtat
    14701 tttatagcac tgccgatatc acgctcaaaa aacaaccact gctattttta gcaaagctta
    14761 tggtatactc cgccgcctta acatttttca ccgcaaattt tcattgcaac atgacgagga
    14821 aaataaatga gtacgcctga tttttctact gccgagaata atcaagaact ggcaaatgaa
    14881 gtctcctgcc tgaaagcgat gctgacgctg atgctgcagg cgatgggaca agctgacgcg
    14941 ggccgcgtga tgttaaagat ggaaaaacag cttgcgctga tcgaagacga aacccaggct
    15001 gcagtatttt ccaaaacggt taagcaaatt aaacaagcct accgtcagta atataaaacc
    15061 ggctgagagc gtgcctttca gccggttttt gcatctggca cgcagaaaga aaagcgatca
    15121 aattaagcca gtagcggccg cgtaacaggc cacctgggtc ttatttggtg cattgatttt
    15181 tttctgcatg tttttctgat ggaaattgac cgtattctca gagattgaca aaatcatcgc
    15241 tatctctgct gatgtcttcc cttccgccgt ccatttcaga atttcttttt cgcgcttgct
    15301 gaaattcatc tctggcgtca tcactatttc atcatttaaa cgcatcagag ccatcagact
    15361 ttcgcgcacc agtaactgca tttttaattg cagttcatca ctaagaatgg gtatttcgcg
    15421 cgtgctgcaa cgggaaaagg acaaaaagcc cagcgcccga tttggcagca ttaaatactg
    15481 agtgacaccg cggcgtaaac catgtgcgtg cgcggcttcc cataatggct gtgcttcgct
    15541 gaataagtca tcattccaca ttaaatggcc ctgactaaag ttttcagggt tcagcacagg
    15601 atcaattgcg agaaaatttt ttgcctgata ataactaacc cacgactcag ggtaattggt
    15661 gtagaaagcc actttaggtc gcgtgaatgg taccgggtgg cgaacacata acgaatagta
    15721 atcgtactcc agctgctgag cctgaagctc tatttcatgg tagacctctt ctgcggcctc
    15781 catcctctga aaacgcaaca gcatcgtgcg acgccaactg aaaaaatccg tatcctgcat
    15841 agtaaaccgc aacgcccctg agagtgagta tcatttataa tgataattca aactatcaca
    15901 taaataagat ttatatataa tttatattat tcaggcaatg aattactttt gcaagccatc
    15961 gcattctctt atgttattaa tgagttatgc tgatttgtta agcagtttta tcacgcttga
    16021 aatggcgtcc cgccccgaca ggtgaatcgt cggggctgat tttttcttat tattgcagca
    16081 gaaacttctc gaggaactgt cgggtgcgag gctgctgggg atcggcaaat aacgcttttg
    16141 ctgccccctg ctcgactatc cgccccaggt ccataaaaat cgcccggtcc gcaacatccc
    16201 gggcaaagct catttcgtgc gtcacaatca ccatcgtgcg cttttcctgc gccagctggc
    16261 ggattgtgtt caggacttcg cccaccagct ctggatccag cgctgacgtt ggctcgtcaa
    16321 acaaaatcac ctcaggacgc attgccagcg cacgcgcaat cgctacacgc tgctgttgac
    16381 cgccagacaa acgacgtgga tagctggttt ctttaccggc cagcccaact ttcgccagca
    16441 gctcgcgagc gcgcgccgtg gcctcttctt tcggttcgcc tttgacgatc accggccctt
    16501 caataatgtt ttccagcacc gtacgatgcg gaaacaaatt aaagttctgg aagacaaacc
    16561 cgacgtgctg acgcaactgg cgaatcagcg atttttgctg acttaatgaa cgtgcagtat
    16621 caatagtgat atcgccaacg gtgatcgttc ccgcttccgg ttgttccagc agatttatgc
    16681 tgcgtagcaa cgtggttttg ccggaaccac tcggaccgat aattgccacc acttcgccag
    16741 gctttacctc gaggtcgata ccgtgcagca ccgtctgacc ttggaatttt ttcaccaggt
    16801 tcttaacttc aatggcactc atttgggttc tctctcctgg cgattaagtt gattctcaaa
    16861 atggttctgc aacgtcgata acacagtcgc catgatccag tagatgagcg aagccgccag
    16921 gtacatggtg aacacctcca gcgtgcgcga ggtaatcaac tgcgcctgac ggaacagctc
    16981 cggcacctgg attgtcgcgg ccagcgacgt atcttttacc agactgataa agctgttcga
    17041 cagcggcggc aacgccaccc gcgccgcctg tggcaaaatc gcgcgacgca tggtctgcca
    17101 cggcgtcata ccaatactgg cagcggcttc ccactgacct ttatcaatag aagaaatggc
    17161 ggcgcgcagc gtttcggcgg cataggcggc agtgttcagc gacagaccaa tcattgctga
    17221 cagaatggga tctaattcga taccaaactg cggcaggccg taatagatca taaacagttg
    17281 tgcgatgagc ggcgtaccac gaaaaataga gatataaaaa cgcgccagcc agcgcacggg
    17341 ccagattggc gacaggcgca tcagcgcgag aataaacccg agtagtaagc caaaaaacat
    17401 gccgccgata ctgagttgca gggtataccc agcccctttc aacaggaacg gcaaggaatc
    17461 aataaccagt tgtatacttt cttgcatgag cgttcccgga gctaaacgtg gggatgatag
    17521 gcgaacagcg caggcgcgcc gccggtatga ataaacagaa tcggcccttc atctttgaag
    17581 cgtttctgac tgataccgtc aatcagcccc gccatcgctt ttccggtata cacaggatca
    17641 agcagaatgc cttccagacg cgccagcaat ttcactgctt ccatgccttc gtcgttcggc
    17701 acgccgtagc caggtgcaaa atagtcatcc cagagtaaaa tttccgctga tgcggtcagc
    17761 tccagttctt tcgcaatcgc ctgttgtagg ttaaccactt tcggcaattg atcggcaacg
    17821 gaacgcgaca cggtcacgcc aatcagttcg ctttcaggca tcaggtgttc cagcccaaca
    17881 gccaatccgg cgtgagttcc ggcactgccc gatgcgacca ccaccgacga aatattaacc
    17941 gccccttcac actgttgcgc gatttccagc gcactctcca cataacccag cgcgcccaga
    18001 gcattagaac cgccaaccgg aatgacatac gggcgaaagc cttgtgcttc gactcgcgtc
    18061 gccagctctt ccagttgggc attgggatcg gtcagtgcgt cgcacatttc aatctgggta
    18121 ttgaacagat tcagcaacaa acgattgccg ttggttaaat agttttctgc ggttgtgcca
    18181 ataggatttt ccagcagcgc cacgcagtgc aggccgagtt tcgccgcaac tgcggcagtc
    18241 tgccgcacat ggttagactg gatcgcgccg gcagtaatgc gtatcggcac cttcgcgcag
    18301 ggcatctgcc gcgagaaatt ccagcttacg taatttattg ccgcccattg ccatgggtgt
    18361 gacgtcatcc cgtttgatga aaatttcccg tccaagataa tcagaaaagc gcggcagata
    18421 ttcgagcggc gttggcgcgc cgataaactc cagccgtgga aaacgggtta aattatgcag
    18481 tggcataata gcctccgatg tgtgttgttg tgattttctt attatgcacg ctgaaaacgc
    18541 gtaaataaaa aaggcgctag tgaaagcgcc cttttttgtc attatgctga ttatttggtc
    18601 acatcagcac caaaccattt ttcggaaagg gcttgcagag tgccatcttt ttgcatttcc
    18661 gcaattgcat cgttcactgc tttcagcagg tcttcatttc ctttacgcag cgccacgcca
    18721 gactcctgac gggagaatgc ttcaccggtt actgccagcg tatcgttggt tttcttcacc
    18781 agatccagcg ccgccagacg gtcaacgagg atcgcatcga tacggcctac gcgcagatcc
    18841 tgatatttgg tcgggtcatc atcataggta cgcacatcga cgccctgaac attctgccgc
    18901 agccactctt catagttggt gcccagaccg acacccactt ttttgccttt cagatcagca
    18961 gccgttttaa tggtgccttc gttacctttt ttcaccagcg cctgaatacc agaaatggtg
    19021 tacggggtag agaaatcgta ttttttcttg cgctcatcag aaatggtgac ctgattaatc
    19081 accacatcaa tacgtttaga gtccagcgac gctagcatac cgtcccattt ggtcggtttt
    19141 agtgacgcct caacgccaag atgttttgcc agctgttggg caaattccac ttcaaaaccg
    19201 gttaatttgc cgtcatcccc ctgaaaactg aacggcggat aagttccttc cagccctacc
    19261 agtagcgtac cgcgctcttt gactttatta agcagacctt catctgcaaa acttttaacg
    19321 ctcatgcccg caaccagcgc cacggccatc acacccatca atgcctgacg tcccagatgt
    19381 gctaatttca tattcacccc gaatgttgtt atgtctgttt gcagtgtaga gccattgttt
    19441 gtaaacacaa aaacaactcc gctacatctt attcttattt aatatatatc agaagcaggc
    19501 aggctggagg atttctgcac aagtccggta cacgtttgac gctgatagtg ttgatacttc
    19561 cgtaatgcga tgcggtaatt gttggtgctg gtggtgggca gccacggggc gaggatttca
    19621 tcaaggaaac cgtcctgcag ctgatcgagg gaaatatttt gctcatgtag atgattcccc
    19681 aaacggcgca aacgaacgac atattcacgc accgtacctg ggctcatttc agtttgctca
    19741 aaaagaaatt gcttaaagcc gataatatcg aaaaaatcac tctgcgtttt gcaatgtaaa
    19801 tcaccgcaaa atcggcacaa tgccgcccat gactgttgtt ccacttgcca gccattttca
    19861 tcaagcagcg tgtccaggcg ggaaatctcg attttattca ctattttgcc gtcgcgaact
    19921 aaggtaatgc gatcgagtaa tttacggcaa tgcgcgcaat gggcctggct gtgtttaaag
    19981 tctttaagat agcggcttaa tggccgtctt ttcaggtgct gcaccatcat taagaactcc
    20041 tggtagtcaa agttaaagtg cggcatttac tgacgttata acttacccag tttagtgctt
    20101 aaccgtttaa tggcctggct gtgtaactga ctgacccgcg attccccgac ctccagcacc
    20161 gcgccaatct ctttgagatt cagctcttcc tggtaataga gggttaatac cagtttttcg
    20221 cgctccggca acgtttcgat agcttccatc acccgctggc gcagattact gtccagcagt
    20281 tgttgtagcg ggttttctcg ctgatgatca tcagtaacca gttcgatgct atcgccgtgc
    20341 tcttcgcgcc actcatcgta ggagaaaagc tggctgttat tggtgtcgag caacatttgg
    20401 cgataatcgg caatatcgat ccctaaacgt tccgctacct cagtttccgt ggcgttgcgg
    20461 ccaagttcct gctccagttg ccctattgcc tgtgccactt cacgcgcgtt gcgtcgcacg
    20521 ctgcgcggca cccagtcacg gctgcgaagt tcatccagca tagcgccacg gatacgctgc
    20581 actgcgtaag ttgtaaatgc cgttccttgt agggcgtcat agcgttcgac ggcattaagt
    20641 aacccaatgc cgcccgcctg tagcagatcg tcaagttcca cgctcgcggg cagtcgaacc
    20701 tgcaggcgca atgcttcgtg acgcaccagc gggacataac gctgccacag cgagtgttta
    20761 tccattacac cttcagcggt atagagtgaa ttcacgataa acagccctgc gttatatgag
    20821 ttatcggcat gattatccgt ttctgcaggg tttttaatcg gacgattagt gggtgaaatg
    20881 aggggttatt tgggggttac cggtaaattc catgcagaaa aaaccccgcc ggtggcgggg
    20941 aagcacgttg ctgacaaatt gcgctttatg ttgccggatg cggcgtaaac gccttatccg
    21001 gcctacaaaa atgtgcaaat tcaataaatt gcaattcacc ttgtaggcct gataagtgca
    21061 gcgcatcagg caatttggcg ttgccgtcag tctcagttaa tcaggttacg acgattaacc
    21121 ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca cggagttacc
    21181 ggcctgctgg atgatctgcg ctttcgacat gttggacact tcggtcgcat agtcggcgtc
    21241 ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc
    21301 agaatccaga cggttctgaa tagcacccag ggatgaacgg aatttgtcga tggagctgat
    21361 agcttcatcc agggcagcca gaggatcttt ggttgcagtg cctgcactgg tggtttcagt
    21421 ggtcagtttg cctgtagagt taacataagc ctgtgcccca acagtagctg caaatttacc
    21481 agttgcacct gcgccatttg aagctacagt tactgtaccg gtatccttat cgacgttgta
    21541 gttgatagtg taagatgcgg ttgtggtgag ctctttgtct gcatcagtat aggtgtcatt
    21601 cgaattacca gcaccattag tattcgcacc accgaaggac gccgttgcag actgaatacc
    21661 tgtattatac ttaattgtcg caccagtaac tgctgtgtta gccgttgcag cactctgcac
    21721 tgtgctcagt acagtatcag cgcttacagt tttggtgtag gttacgccag aagttgccgc
    21781 tgcaccaaag ttataagttg tgccagacaa ctggatagaa ccagttgtcg ctaaggtact
    21841 accgctagca aacagatcgg acaatttcgc tgaagatgca atgccggcgt tgtttgtaga
    21901 taggttacca gtggcgtcca ggtacgcggc tttaccaccg atggtcacat cgccattagc
    21961 atctacatca aacttcacat caccagtact acgagtataa acacctgatg cagtagtgcc
    22021 agccgctggt ttcagagtat tcgcaaagtt aacaacatcg ccatctgcaa ctgttgcttg
    22081 agtggtgaag ttccctttag ccgcatcata agtatagatc gcagcactcg agccagtagt
    22141 agtaactgta tctccggttt tcaggcgaga cagtgcatcg ctagcgctga gtgctgtatt
    22201 gtttgtggtc acagcataag gacctgttcc cgttgcacca gcagcggtca gatcgctgac
    22261 tgtagcagct ttgttcgcaa tagtgccttt gccattcacg ttaaaaccat tcagccccag
    22321 cgtatctgag tcaattttct tcagatcaat cgtgatagtc tggccgtcat tcgcaccaac
    22381 ctgaattttc attgaaccgt ctttcgccag tacgttcacg ccgttgaact gggtctggcc
    22441 agatacgcgg tcaatttcgt ccagacggga tttgatttcg tcctgaatgg agtccagatc
    22501 cgaatcggag ttagtcccgg tagaagcctg aaccgtcagt tcaagaatac gctgtaagtt
    22561 gttgttgatt tcggacagcg cgccttcagt ggtctgcgca acggagatac cgtcgttggc
    22621 gttacgggcc gcctgagtca ggcctttaat attagaagta aaacggttag caatcgcctg
    22681 acctgcggcg tcatccttcg cgctgttaat acgcaagcca gaagacagac gctcgataga
    22741 actcgacagc gcagactggt tcttgttgat attattttga gtgatcagcg agaggctgtt
    22801 ggtattaatg acttgtgcca tgattcgtta tcctatattg caagtcgttg attacgtatt
    22861 gggtttccac ccgtcggctc aatcgccgtc aaccctgtta tcgtctgtcg taaaacaacc
    22921 tttagaattt ttttcacaaa cagccatttt ttgttagtcg ccgaaatact cttttctctg
    22981 ccccttattc ccgctattaa aaaaaacaat taaacgtaaa ctttgcgcaa ttcaggccaa
    23041 taaccccggt attcgtttta cgtgtcgaaa gataaaagga aatcgcatgg caagtatttc
    23101 atcgctggga gtcgggtcag gtctggattt aagttcaatc cttgatagcc tcaccgccgc
    23161 gcaaaaagcg acgctaaccc ctatttcaaa tcagcaatcg tcgtttaccg ctaaacttag
    23221 cgcctacggt acgctgaaaa gcgcgctgac gactttccag accgccaata ctgcattgtc
    23281 taaagccgat cttttttccg ccaccagcac taccagcagc accaccgcgt tcagtgccac
    23341 cactgcgggt aacgccatcg ccgggaaata caccatcagc gtcacccatc tggcgcaggc
    23401 gcaaaccctg accacgcgca ccaccagaga tgatacgaaa acggcgatcg ccaccagcga
    23461 cagcaaactc accattcaac aaggcggcga caaagatccg attaccattg atatcagcgc
    23521 ggctaactca tcgttaagcg ggatccgtga tgctatcaac aacgcaaaag cgggcgtaag
    23581 tgcgagcatc attaacgtgg gtaacggtga atatcgtctg tcagtcacat caaatgacac
    23641 cggccttgat aatgcgatga cactctcggt cagcggtgat gatgcgctac aaagttttat
    23701 gggctatgac gccagtgcca gcagcaacgg tatggaggtc tcggtcgccg ccaagaatgc
    23761 gcagctgacg gtcaacaacg tcgccattga gaacagcagc aacaccatca gcgacgcgct
    23821 ggaaaacatc accctgaacc tgaacgatgt caccacgggc aaccagacgc taaccatcac
    23881 tcaggacacc tccaaagcgc aaacggcgat taaagactgg gtgaatgcct ataactcgtt
    23941 aatagatacc ttcagtagcc tgaccaaata caccgccgta gatgcgggag ctgatagcca
    24001 gagttctagc aatggcgcac tgctcggcga ctccacgctg cggacgattc agacgcagtt
    24061 gaagtcgatg ctgagtaata ccgtcagttc ttccagctat aaaacgctgg cgcagattgg
    24121 tatcacgacc gatcccagcg atggcaaact ggaactggat gccgacaaac tcaccgctgc
    24181 actgaaaaaa gatgccagcg gcgtaggtgc attgattgtt ggcgatggta aaaaaaccgg
    24241 catcacgacc accatcggca gcaacctgac cagttggctt tcgacaacgg gcattattaa
    24301 agccgctacc gatggcgtta gtaagaccct gaataagtta actaaagact acaacgccgc
    24361 cagcgatcgc attgatgcgc aggtcgcgcg ctacaaaaaa caatttaccc aactggacgt
    24421 tttaatgacc tcgttaaaca gcaccagcag ctacttaacg cagcagttcg aaaacaacag
    24481 taattccaag taatcattat tcatcgggag acaggtcatg tacgcggcaa aaggcaccca
    24541 ggcctatgca caaattggcg tcgaaagcgc cgtaatgagc gccagccagc agcagctggt
    24601 caccatgcta tttgatggag tgctgagcgc actggttaga gcgcgcctgt ttatgcagga
    24661 caacaatcag caaggcaaag gcgtctcttt gtcaaaagcg atcaacatca ttgagaacgg
    24721 actgcgggtg agtcttgatg aagagagcaa agacgaacta acccaaaact tgattgctct
    24781 ttatagctat atggtcaggc gcttgctgca agccaattta cgcaacgatg tctccgcagt
    24841 cgaagaagtg gaagcattaa tgcgcaatat tgccgatgcc tggaaagagt cgttactctc
    24901 cccttctttg attcaggacc cagtctgatg aaccatgcac cgcatttgta tttcgcctgg
    24961 caacaactcg tcgaaaaaag ccagctcatg ttacgcctgg caacggaaga acaatgggac
    25021 gaactcatcg ccagcgaaat ggcgtatgtg aatgcggtgc aggagtatag cacacctgac
    25081 tgaagaggtt gccccgtcca ccacgatgca ggagcagctc cgcccgatgc tgcgcctgat
    25141 tctcgacaac gaaagcaagg taaagcagtt attacagatt cgtatggatg aactggcgaa
    25201 actggtcggt cagtcatcgg tgcaaaaatc ggtgttaagt gcctatggcg atcagggcgg
    25261 ctttgtgctg gctccgcaag ataacttctc gtgaatctta acgagctaat ggttcgcgaa
    25321 taatccgatt acggctacgc ttctaatgtt ccccttgaat ggagtcgaag aatgcgtaat
    25381 cccacgctgt tacaatgttt tcactggtat tacccggaag gcggtaagct ctggcctgaa
    25441 ctggccgagc gcgccgacgt ttttaatgat attggtatca atatggtctg gttgccgccc
    25501 gcctataaag gcgcatcggg cgggtattcg gtcggctacg actcctataa tttatttgat
    25561 ttaggcgagt ttgatcagaa aggcagcatc cctactaaat atggcgataa agtacaattg
    25621 ctggccgcca ttgacgccct gaaacgtaat gacattgcgg tgctgttgga cgtggtagtc
    25681 aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgac
    25741 gaccgtacgc aaattgatga agaaatcatt gagtgcgaag gctggacgcg ttacaccttc
    25801 cccgcccgtg ccgggcaata ctcacagttt atctgggatt tcaaatgttt tagcggtatc
    25861 gaccatatcg aaaaccctga tgaagatggc atatttaaaa ttgttaacga ctacaccggc
    25921 gaaggctgga acgatcaggt tgatgatgaa ttaggtaatt tcgattatct gatgggcgag
    25981 aatatcgatt ttcgcaatca tgccgtgacg gaagagatta aatactgggc gcgctgggtg
    26041 atggaacaaa cgcaatgcga cggttttcgt cttgatgcgg tcaaacatat tccggcgtgg
    26101 ttttataaag agtggatcga acacgtacag gaagttgcgc caaagccgct gtttattgtg
    26161 gcggagtact ggtcgcatga agtcgataaa ctgcaaacgt atattgatca ggtggaaggc
    26221 aaaaccatgc tgttcgatgc gccgctgcag atgaaattcc atgaagcatc gcgcatgggg
    26281 cgcgactacg acatgacgca gattttcacg ggtacattag tggaagccga tcctttccac
    26341 gccgtgacgc tcgttgccaa tcacgacacc caaccgttgc aagccctcga agcgccggtc
    26401 gaaccgtggt ttaaaccgct ggcgtatgcc ttaattttgt tgcgggaaaa tggcgttcct
    26461 tcggtattct atccggacct ctacggtgcg cattacgaag atgtcggtgg tgacgggcaa
    26521 acctatccga tagatatgcc aataatcgaa cagcttgatg agttaattct cgcccgtcag
    26581 cgtttcgccc acggtgtaca gacgttattt ttcgaccatc cgaactgcat tgcctttagc
    26641 cgcagtggca ccgacgaata tcccggctgc gtggtggtca tgtcgaacgg ggatgatggc
    26701 gaaaaaacca ttcatctggg agagaattac ggcaataaaa cctggcgtga ttttctgggg
    26761 aaccggcaag agagcgtaat aaccgacgaa cacggcgaag caaccttctt ttgcaacggc
    26821 ggcagcgtca gcgtgtgggt tatcgaagag gtgatttaaa ttcatccccg gcggcaagcc
    26881 ggggagattt cattacggca gtggcgtcgg taacggcgct ttatccagcg cggcggcaca
    26941 ctctgccgtc gggcgatcca cacgctccat cgtcatacca tcgtactcaa tggtgttacc
    27001 gtcacgctca acctcgtaca gctcacgttt tacggtaacg ttagtgagat catcagacat
    27061 caatgccaac ttacccggca cagcaatcac ccgctgccac tgacggcaat ccagagtatc
    27121 accctcttta gtgacaatca ggctgcctat cgcttccgga ctgaccagcg cacgttgcgg
    27181 ccctttggtc tgccagtagc ccgccagcca gtccggcgcg ggggttttca cgacattgtt
    27241 atagttttcg acttcggcac agcccgccag caacatgagc gcgcctgcaa ttgcgagttt
    27301 tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg gcattaaagc cctgatgtcg
    27361 ccagcctttc caggttcaga gagaaatttg atctaactat tatttttgtg aattattacc
    27421 ccgaaaataa atttgcatga tgtatgattc gcgactcttg tttcaaacct tctgagttca
    27481 gaggctacat tcatgtcatg gcagcaattc aaacacgcct ggttgattaa attctgggca
    27541 cccatccctg cggtcatcgc ggcgggtatt ctctccactt actattttgg cattactggc
    27601 accttttggg ctgtcacggg tgaatttacc cgttggggcg gccagctcct gcagctgttt
    27661 ggcgtccatg ctgaagagtg gggttatttt aaaattatcc atctggaagg atcgccatta
    27721 acccgcatcg acgggatgat gatcctcggc atgtttggcg gctgctttgc cgcagcgctg
    27781 tgggccaaca atgtcaaact gcgcatgcca cgcagccgta tccgcattat gcaggccatt
    27841 attggcggta ttatcgctgg ttttggcgcg cgtctggcaa tgggctgtaa cctggcggcg
    27901 ttctttaccg gtattcctca gttctcgctg catgcctggt tctttgccat cgccactgcc
    27961 attggttcat ggtttggcgc acgctttacc ctgctgccca tcttccgtat tcccgtgaaa
    28021 atgcagaaag tttctgccgc ctcaccgctg acgcaaaaac cggatcaggc gcggcgtcgt
    28081 tttcgtctcg ggatgctggt ctttttcggt atgctgggct gggcgctgct cacggcgatg
    28141 aaccaaccaa aactggggct ggcaatgctg tttggcgtcg gctttggttt actgattgaa
    28201 cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt ggatcaccgg acgtacccat
    28261 atggcgaaag caatcattat cggtatggcg gtgagtgcca tcgggatctt cagttacgta
    28321 cagttaggcg ttgaacccaa aatcatgtgg gcgggaccaa acgcggtaat tggtggttta
    28381 ctgtttggtt ttggcatcgt gctggcaggc ggctgcgaaa ccggctggat gtaccgcgcg
    28441 gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca atgtgatcgg ctcgacgatt
    28501 ctggcgtatt actgggatga tttcgctccg gcgctggcca ccgactggga caaaatcaac
    28561 ctgctgaaaa cctttggccc gatgggtggc ctgctggtga catatttgct gttgtttact
    28621 gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc gccgtgcggc accgcagact
    28681 gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg ccttgatatg gtgggtgaac
    28741 cctgccctta tccggcagtc gcaacccttg aggcgatgcc gcagttaaaa aaaggggaaa
    28801 tcctggaagt ggtgagcgac tgtccacagt cgatcaataa tattccactg gatgcgcgta
    28861 atcacggcta tacggtgcgg gatattcagc aagacgggcc gaccattcgt tatttaattc
    28921 agaagtaatc cctcattccc gctgtggccc acagcgggaa catcgttgcc tctgacacat
    28981 agattttacc cgccccctct cctattcttt tcagtatgat ctcaggaggt ttctatgtgt
    29041 ggacgctttg cccaatccca aacgcgtgaa gattaccttg cgcttctcgc ggaagatatt
    29101 gaacgcgata ttccctacga tcccgaaccc attggcagat acaacgtcgc gccgggaacc
    29161 aaagttcttc tgctcagtga acgtgatgaa caccttcatc tggatccggt tttctgggga
    29221 tatgctcccg gatggtggga taaaccgccg ctgattaacg cccgcgtaga aactgccgca
    29281 accagtcgca tgtttaaacc gctctggcaa catggtcggg caatctgttt tgccgatggc
    29341 tggtttgagt ggaaaaaaga aggcgacaaa aaacagcctt tttttatcta tcgtgctgat
    29401 ggacaaccta tttttatggc cgcgataggt agcacaccat ttgagcatgg tgacgaagcc
    29461 gaaggatttt taattgtcac tgctgcagca gaccagggtc tggtagacat tcatgaccgc
    29521 cgcccgctgg ttctgtcgcc agaagctgca caggaatgga tgcggcaaga gattggcggt
    29581 aaagaagcat cagaaattgc taccaacggc tgtgtgccag caaaccagtt cacctggcac
    29641 ccggtatcgc gcgcggtagg taatgtcaaa aaccagggcg cggagttaat tcaacctgtt
    29701 tgttgacatc ctccacgccc tg
//
LOCUS       DAJTNN010000030        29253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTNN010000030 DAJTNN010000000
VERSION     DAJTNN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.030"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..155
                     /locus_tag="OO150_001211"
     CDS             <1..155
                     /locus_tag="OO150_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HCR5515421.1"
                     /translation="NYYGGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELTL
                     VKSFDW"
     gene            430..3279
                     /gene="rcsC"
                     /locus_tag="OO150_001212"
     CDS             430..3279
                     /gene="rcsC"
                     /locus_tag="OO150_001212"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5515422.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGGEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGEPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3479..4129)
                     /gene="rcsB"
                     /locus_tag="OO150_001213"
     CDS             complement(3479..4129)
                     /gene="rcsB"
                     /locus_tag="OO150_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5515423.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4146..6818)
                     /gene="rcsD"
                     /locus_tag="OO150_001214"
     CDS             complement(4146..6818)
                     /gene="rcsD"
                     /locus_tag="OO150_001214"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5515424.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7557..8687
                     /gene="ompC"
                     /locus_tag="OO150_001215"
     CDS             7557..8687
                     /gene="ompC"
                     /locus_tag="OO150_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5515425.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINSRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8799..9854
                     /gene="apbE"
                     /locus_tag="OO150_001216"
     CDS             8799..9854
                     /gene="apbE"
                     /locus_tag="OO150_001216"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5515426.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GINGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9928..10980
                     /gene="ada"
                     /locus_tag="OO150_001217"
     CDS             9928..10980
                     /gene="ada"
                     /locus_tag="OO150_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5515427.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCPVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVLANAIGKPKAVRAVASACAANKLAIVIPCHRVVGGDGTLSGYRWGV
                     SRKAQLLRREAENEER"
     gene            10980..11630
                     /gene="alkB"
                     /locus_tag="OO150_001218"
     CDS             10980..11630
                     /gene="alkB"
                     /locus_tag="OO150_001218"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5515428.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTIHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFYPLTTDCRYNLTFRQAGKKE"
     gene            11706..13349
                     /gene="yojI"
                     /locus_tag="OO150_001219"
     CDS             11706..13349
                     /gene="yojI"
                     /locus_tag="OO150_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5515429.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLMAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13567..15213
                     /gene="mqo"
                     /locus_tag="OO150_001220"
     CDS             13567..15213
                     /gene="mqo"
                     /locus_tag="OO150_001220"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR5515430.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15363..15851)
                     /gene="eco"
                     /locus_tag="OO150_001221"
     CDS             complement(15363..15851)
                     /gene="eco"
                     /locus_tag="OO150_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5515431.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            16259..16753
                     /gene="napF"
                     /locus_tag="OO150_001222"
     CDS             16259..16753
                     /gene="napF"
                     /locus_tag="OO150_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5515432.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16743..17006
                     /gene="napD"
                     /locus_tag="OO150_001223"
     CDS             16743..17006
                     /gene="napD"
                     /locus_tag="OO150_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5515433.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            17003..19489
                     /gene="napA"
                     /locus_tag="OO150_001224"
     CDS             17003..19489
                     /gene="napA"
                     /locus_tag="OO150_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5515434.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTQDATDPLSKETDFKKCAVKLEKV"
     gene            19496..20191
                     /gene="napG"
                     /locus_tag="OO150_001225"
     CDS             19496..20191
                     /gene="napG"
                     /locus_tag="OO150_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5515435.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            20178..21041
                     /gene="napH"
                     /locus_tag="OO150_001226"
     CDS             20178..21041
                     /gene="napH"
                     /locus_tag="OO150_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5515436.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            21038..21487
                     /gene="napB"
                     /locus_tag="OO150_001227"
     CDS             21038..21487
                     /gene="napB"
                     /locus_tag="OO150_001227"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5515437.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            21497..22099
                     /gene="napC"
                     /locus_tag="OO150_001228"
     CDS             21497..22099
                     /gene="napC"
                     /locus_tag="OO150_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5515438.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            22118..22735
                     /gene="ccmA"
                     /locus_tag="OO150_001229"
     CDS             22118..22735
                     /gene="ccmA"
                     /locus_tag="OO150_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5515439.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            22732..23394
                     /gene="ccmB"
                     /locus_tag="OO150_001230"
     CDS             22732..23394
                     /gene="ccmB"
                     /locus_tag="OO150_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5515440.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            23436..24173
                     /gene="ccmC"
                     /locus_tag="OO150_001231"
     CDS             23436..24173
                     /gene="ccmC"
                     /locus_tag="OO150_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5515441.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            24170..24379
                     /gene="ccmD"
                     /locus_tag="OO150_001232"
     CDS             24170..24379
                     /gene="ccmD"
                     /locus_tag="OO150_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5515442.1"
                     /translation="MTLAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            24376..24855
                     /gene="ccmE"
                     /locus_tag="OO150_001233"
     CDS             24376..24855
                     /gene="ccmE"
                     /locus_tag="OO150_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5515443.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24852..26801
                     /gene="ccmF"
                     /locus_tag="OO150_001234"
     CDS             24852..26801
                     /gene="ccmF"
                     /locus_tag="OO150_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5515444.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     MLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIAIASLLSGRLDSTYARFTRPWTLAAW
                     IFLTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKA
                     WTLLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAA
                     RGHKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPF
                     FNTMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKV
                     VAMTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQN
                     YSVERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEK
                     RYYNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMAL
                     GGLLCLFDPRYRKRVSPQKTAPEAV"
     gene            26798..27355
                     /gene="dsbE"
                     /locus_tag="OO150_001235"
     CDS             26798..27355
                     /gene="dsbE"
                     /locus_tag="OO150_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5515445.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            27352..28404
                     /gene="ccmH"
                     /locus_tag="OO150_001236"
     CDS             27352..28404
                     /gene="ccmH"
                     /locus_tag="OO150_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5515446.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(28439..29086)
                     /gene="narP"
                     /locus_tag="OO150_001237"
     CDS             complement(28439..29086)
                     /gene="narP"
                     /locus_tag="OO150_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5515447.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPSFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         6682 a         7219 c         8104 g         7248 t
ORIGIN      
        1 tcaactacta tggtgggcgc agcgaaacgg aattaaccac cgcccaactg acgctgatca
       61 ccgatgaagg atcggtcaat gagaaacagg aaacatttat tgtaccgatg cgtaatgctg
      121 gcgagctgac gctggtgaaa agttttgact ggtgatccat cccttcacac aacctggctc
      181 tctctttttt cagataagac tgctgggcga tctgccgacg ccaggatata accattaaca
      241 agcgtcggca gattggtcat ttcttatttt tcgtactgag aactgtccat ctactccggc
      301 tttcctggtt aaggcaatgg tttctaagcg atacgcatta ttgcaataat gacagaacat
      361 tgcggtaaca cgcttttacc gctaccttaa ccacactcca tcggtcacct gaggcggagc
      421 ttcgcccctt tgaaatacct tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg
      481 ttcagagcat tggcgttagt gctctggctg ttgattgctt tttcatccgt tttttacatc
      541 gttaatgcgt tacatcagcg agaatcggaa attcgtcagg aatttaatct gagttccgat
      601 caggctcagc gctttattca acgcacctct gatgtgatga aagagctgaa gtacatcgcc
      661 gaaaatcgct tatcggcaga aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg
      721 gatgtgcctg cgtttgaacc gctgtttgcc gactccgatt gttccgcaat gagtaacacc
      781 tggcgaggtt ctctggagtc attggcgtgg tttatgcgct actggcgcga taatttttct
      841 gcggcttacg atctaaaccg ggtattttta atcggcagcg ataacctctg catggccaat
      901 ttcggtctgc gtgatatgcc agtggaacgc gataccgcgt tgaaagcttt gcatgaacgc
      961 atcaataaat atcgaaatgc accacaagat gatagcggca gtaacctcta ctggatcagc
     1021 gaaggtccgc gccctggcgt cgggtatttt tacgcgttga cgccagttta tctggcgaac
     1081 cggttgcagg cgcttttggg gggcgagcag accatccgga tggagaactt tttcttaccg
     1141 ggtacgttgc cgatgggggt taccattctt gatgaaaatg gtcataccct gatttcgctt
     1201 accggaccag aaagtaaaat taagggcgag cctcgctgga tgcaggaacg ctcctggttt
     1261 ggctatacgg aagggttccg ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta
     1321 agcatcgtgt attcggtgcc ggttgataag gtgctggaac gcattcgcat gttgatcctt
     1381 aacgcaattt tgctgaatgt gcttgccgga gctgcattgt ttactctcgc acggatgtac
     1441 gagcgacgta ttttcattcc ggcggaaagc gacgccctgc gactggaaga acatgagcag
     1501 ttcaatcgca agattgtcgc ctccgcgcca gtgggtatct gcattttgcg taccgctgat
     1561 ggcgtcaata ttttaagtaa cgaactggcg catacctatc tcaatatgct tacgcatgag
     1621 gaccgccaac gactgacgca aattatctgt gggcagcagg tcaattttgt tgatgtcctg
     1681 accagcaaca ataccaatct gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac
     1741 gtggccattt gtgtgctggt ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag
     1801 gagatggcac aagcagcgga acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc
     1861 agtcatgagc tgcgaacgcc gctgtatggc attatcggta acctggatct gttgcaaacc
     1921 aaagagttac cgaaaggcgt cgatcggctg gtgacggcaa tgaacaactc ttccagcctg
     1981 ttgttgaaaa ttatcagcga tattctcgat ttctcgaaga ttgaatcgga acagttgaag
     2041 atcgaaccgc gtgagttttc accgcgtgaa gtgatgaacc acatcaccgc caactattta
     2101 ccgctggtgg tacgcaagca gttaggcttg tactgcttta ttgaaccgga tgtgccagtg
     2161 gccttaaatg gcgacccgat gcgtttacag caggtcatct ccaacctgtt gagtaacgcc
     2221 ataaaattca ccgataccgg ctgtatagtt ttgcatgtcc gcgctgatgg cgattatctc
     2281 tctatccgtg ttcgcgatac cggcgtgggg attccggcga aagaagtggt gcgcttgttt
     2341 gatcccttct tccaggtcgg aacgggcgta cagcgaaact tccaggggac cggtctgggt
     2401 ctggcgattt gtgaaaaact gatcagcatg atggacggcg atatctcggt agattcagaa
     2461 ccgggaatgg gcagccagtt taccgtgcgt attccgttgt acggcgctca gtacccgcag
     2521 aaaaaaggcg tggaagggtt gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg
     2581 ctctgtcagt tcctagaaac tagtttgcag cgcagcggca tcgtcgttac aacatacgaa
     2641 gggcaggaac cgactcccga agatgtgtta attactgacg aggtagtgag taaaaaatgg
     2701 cagggtagag cggtagtgac cttctgtcgt cgccatattg gtattccgct ggagaaagcg
     2761 ccaggggagt gggtacacag tgtggctgcc ccgcatgagc taccggcatt gttggcgcgt
     2821 atttatttga tcgagatgga gagcgacgat cctgctaacg ctctgccgtc gacggacaaa
     2881 gcggtcagcg ataatgacga tatgatgatt ctggtcgtgg atgatcatcc gattaaccga
     2941 cgtctgctgg cagatcagtt gggatcgttg ggctatcaat gtaaaaccgc gaatgatggt
     3001 gtcgatgcgc ttaatgtact tagcaagaat catattgata tcgtgcttag cgacgtcaac
     3061 atgccaaata tggatggtta ccgcttgacg caacgcattc gtcagttggg actgacgttg
     3121 ccggtaatcg gagtaactgc taatgcgttg gctgaagaga agcagcggtg tctggagtcc
     3181 ggtatggaca gctgcctgtc gaagccggta acgctggatg tgataaaaca gacgctgacg
     3241 gtatatgccg agagggtcag gaaatcgcgg gaatcgtagg acggataagg tgtttatgtc
     3301 gcatctggca ttcagtgcaa atgccagatg cgacgctgac gcgtcttatc tggcctactt
     3361 tgatgtaatc tgttgaaata atgggaatcg taggacgcat aaggcgttta cgccgcatcc
     3421 ggcaatcggt gcaaatgcta gataagacac taacgcgtct tatctggcct acaggtgatt
     3481 agtctttatc tgccggactt aacgtcactg aagagagata attcagcagg gcgatatcgt
     3541 tctcgacacc cagcttcatc atcgcagatt tcttctggct actgatggtt ttaatactgc
     3601 ggttcagctt tttagcgatc tcggtcacca agaagccttc cgcaaacagg cgcagaactt
     3661 cactctcttt tggcgagaga cgcttgtcac cgtaaccacc agcactgatt ttttccaaca
     3721 ggcgagaaac gctttccggg gtaaatttct tccctttctg cagcgcggcg agagctttcg
     3781 gcagatcggt cggtgcacct tgtttcagca cgatcccttc gatatccaga tccaataccg
     3841 cactaagaat cgccgggttg ttgttcatag tcagaacaat gatcgacagg cttgggaaat
     3901 ggcgcttgat gtacttgatt aaggtaatgc catcgccgta cttatcgcca ggcatggaga
     3961 gatcggtaat caacacatgc gcatccagtt tcggcaggtt gttgatcagt gctgtagagt
     4021 cttcaaattc gccgacaaca ttcacccact caatttgctc aagtgatttg cgaataccga
     4081 acaagactat cggatggtca tcggcaataa ttacgttcat attgttcatg taataggcta
     4141 ccttgctaca gcaagctctt gacataactg tcaatgtcgc tgatgtattt ttctattcct
     4201 ggaacatcct tctcacgaat cagatgttcc agcgtttcac ataactgctt gccgggtacc
     4261 agatttagca tggcaaatac gcctttaaga cgatgagccg tttgggctaa cgcagcaaag
     4321 tcactggttg ctgcttcagt atacagcctc ttaacatcat ccggtactgt gtctacaaag
     4381 agcgcataat agccgctggc atggagttgc gcattttcat ctccgcccag aggcgattct
     4441 gtcacctctt cctgcgccag ttgcacttca attaattgta agaccgcttc ctgcatagcg
     4501 ttgctcatat tgaagttgac gcacaattga ccaggcccaa tttcccgtac gccagactca
     4561 tcatcgctta aaagcaagcc agaggcagta agattagacg gattatccgt taaaaagata
     4621 tcataatctt gactaattaa tctttcatcg ggtgtgatac aggttgcacc ccaattttct
     4681 aactggcgag tgacaatatt ccgaatttct gccgaagtaa catccaccat tacgcagaca
     4741 tcatccagta aacgctcttc ttcctcttca acttccgggt cagcggcgag cattttgata
     4801 tgcacagagt agcgtgtacc aagcccatcc cgcgttttga tgtttaaatg accgcccagt
     4861 ttacgcgcca gttgatcgct cagccagaat gccagcgggt ccgccttgcc atagcgatcg
     4921 ttttggggct ggttgataaa cgggaagtgc aaattatcca tttcatgaat acttacgcct
     4981 tctcccgtgt ccagaatgcg gaatgtcagg cggtcttcag aggactcatc ctgatcaacc
     5041 tcaagggtga tttttcccaa ttgcgttgag gtcacggcat attgcatcag tagcagcaaa
     5101 atacgtcgta aggcatcgcg atcgccgcgg cgcatatcgt gtgctttcag atgattgtta
     5161 atcagcagtt gcagaccttt acgcttgatg gcaggcaaca ctgaaggcac aacttcatca
     5221 attaaatcct gcacggagaa cagcaccgtc tcacttttcc agctatcgtc cgcaagcatg
     5281 ttcgctaact gtatttcatc gaccagccgc accagcacat ctgcctgatt cgccagttgt
     5341 ttgctttccg gggcgttgag tttagccgcg ctctccgcca gggactgtgc gggttctttc
     5401 agcgcatcgc caatgttttt cataaaggtc atccgcccct gctggttttt ctcatacaga
     5461 cgctgcgcct gcttgagttt cttgtttacc agcacttcgc gatcctgatc gcgaataatg
     5521 aaaatttgtg tgcgcggcgc gacctggctg cggaacatgc ggatctcata cagctcgtta
     5581 ttgatcgtcg cctgaataat cccctgatgc tgttccgcca tggtggtgat gttttgcaga
     5641 ttcaaatgcg gcagcaaatg atcggcaatt ttgttactta tgacagtgcg gttcgattcc
     5701 tgatcgtgaa ccagcaggcc gagcggcagc agtgagacta tctcttcatt aattgcccgt
     5761 aaaatgcgca attcgttatt gaccgccgtg ctgggtagac tttctgtact gcggctggag
     5821 aaatggcgga atgtggtata gccaaataac gccagcgcca gcaaaccgat gttcagcagc
     5881 agtggcagca gaatgttttg caacgtatcc agcaataagg tgccataagg aacctgccag
     5941 accaggcgca tatcggtaga gttgagtgcc gaggagattt caatcttcgt actgttaaag
     6001 tggatactga cgctatccgt cccttctttc tcattatcat tgtctcccgt tgccgtcgcg
     6061 tctggctcaa ggcggaaact gtccagcggc atacccggtg ggatcaaatc attaatcggt
     6121 agatcaaaag ctacgaccgt tgccagatgt cccggctggt tgaacgtggt acgcaaggta
     6181 aagtaatgac cgttctgcca ggccaggcgg cgcatgttag aaaaactttc gcgttcatcg
     6241 agggcgttgg cctgttgcaa catctctgca cgacgtgaat caacaatgtc gctgatggtc
     6301 gattctttaa atccggaggt gagatctttg aggggcaggg ttgagatcag caccagactg
     6361 ttatcctgac cattcaggta atacatcgac cacggtacat tttctgcgcc ccacaatgtg
     6421 tccagatagg tggacatccg ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag
     6481 attaacgctt ccgttttgcg gcgcggtttc tccagatagt agacatcctg tttcaggcgc
     6541 gtctcttgta aaccttcgcc ggaggagggg gaggttgtcg cggcaatgtt gtcgtagatc
     6601 tgccaggtca cgtaacgcca ggtattgacg cgcttttgga tagcgtgggt aatgtcgaca
     6661 atctggtaac ttttatcttt cagccatgcg ttaacggcgc tttgtaccat tacacccatc
     6721 gtcaccagta acacaatgat caacagtaaa aagaagcggg taatgctccc cggtaggagt
     6781 gaaaagcggg tcgtggccgt tgtctctttc tgacgcattc gtgtttatga cctgttaaaa
     6841 cttcgcgaag tgatagggca gtataaaggg tacagtgtga aattccagac tcttacctga
     6901 ttgatgattc tctattctcc cgtgatattt atcatcctgc attgggggaa atagatgtac
     6961 aagcgccatt ttgctgtaca aataacctac aaaaagccca acgaaattaa ttgcaaataa
     7021 acgatgattg ctggaaatta tgctgatgtt aattatttgt gaaatagtta acaagcgtta
     7081 tagtttttct gtggtagcac agaataatga aaagtgtgta aagaagggta aaaaaaaccg
     7141 gatgcgaggc atccggttga aataggggta aacagacatt cagaaatgaa tgacggtaat
     7201 aaataaagtt aatgatgata gcgggagtta ttctagttgc gagtgaaggt tttgttttga
     7261 cattcagtgc tgtcaaatac ttaagaataa gttattgatt ttaaccttga attattattg
     7321 cttgatgtta ggtgcttatt tcgccattcc acaataatct taaaaagttc ccttgtattt
     7381 acattttgaa acatctatag cgataaatga aacatcttaa aagttttagt atcatattcg
     7441 tgttggatta ttctgcattt ttggggagaa tggacttgcc gactgattaa tgagggttaa
     7501 tcagtatgca gtggcataaa aaagcaaata aaggcatata acagagggtt aataacatga
     7561 aagttaaagt actgtccctc ctggtcccag ctctgctggt agcaggcgca gcaaacgctg
     7621 ctgaagttta caacaaagac ggcaacaaat tagatctgta cggtaaagta gacggcttgc
     7681 actatttctc tgacaacaag tcagaagacg gcgaccagac ctatgtacgt cttggtttca
     7741 aaggcgaaac tcaggttact gaccagctga ccggttacgg ccagtgggaa tatcagatcc
     7801 agggcaatac ctctgaagac aacaaagaaa actcctggac ccgtgtggca ttcgcaggtc
     7861 tgaaattcca ggatgtaggt tctttcgact acggtcgtaa ctacggcgtt gtttacgacg
     7921 taacttcctg gaccgacgta ctgccagaat tcggtggcga cacctacggt tctgacaact
     7981 tcatgcagca gcgtggtaac ggcttcgcga cctaccgtaa caccgacttc ttcggtctgg
     8041 ttgacggtct gaactttgct gttcagtacc agggcaaaaa cggtagcgta agcggcgaag
     8101 gcatgaccaa caacggtcgt ggcgctctgc gtcagaacgg cgacggtgtc ggcggttcta
     8161 tcacttatga ttacgaaggc ttcggtatcg gtgctgcagt ttccagctcc aaacgtactg
     8221 atgatcaaaa tggtagctac atcagcaatg gtgtagttcg taactacatc ggtactggcg
     8281 accgtgctga aacctacact ggtggtctga aatacgacgc taacaacatc tacctggctg
     8341 ctcagtacac ccagacctac aacgcaactc gcgtaggttc cctgggttgg gcgaacaaag
     8401 cacagaactt cgaagctgtt gctcagtacc agttcgactt cggtctgcgt ccgtctgtag
     8461 catacctgca gtctaaaggt aaaaacctgg gtgtcatcaa tagtcgtaac tacgacgacg
     8521 aagatatcct gaaatatgtt gatgttggcg cgacctacta cttcaacaaa aacatgtcca
     8581 cctatgttga ctacaaaatc aacctgctgg acgacaacca gttcactcgt gacgctggca
     8641 tcaacactga taacatcgta gctctgggtc tggtttacca gttctaatct cgattgatat
     8701 cgaacaaagg gcctgcgggc ccttttttca ttgttttcag cgtacaaact cagttttttg
     8761 gtgtactctt gcgaccgttc gcatgaggat aatcacgtat ggaaataagc tttacccgcg
     8821 tggcactgct ggctgccgcg ctcttctttg ttggttgcga tcaaaaacca caacccgcca
     8881 aaacccacgc tactgaagtt accgttcttg aaggcaaaac catgggtacc ttctggcgtg
     8941 ccagcatccc gggcattgat gccaaacgca gtgccgaact taaagaaaag attcagaccc
     9001 agctggacgc cgacgatcag ctgctttcga cctataaaaa agattccgcg ctgatgcgct
     9061 ttaacgactc gcaaagtttg tcgccgtggc cagtaagtga agcgatggcc gaaatcgtca
     9121 ctacctcgct gcgcattggc gcgaagaccg atggtgcgat ggatattacc gtcgggccgc
     9181 tggtaaatct gtggggcttt ggcccggaac aacagccggt tcaaattccg agccaggaac
     9241 agatcgacga gatgaaagcc aaaactggtt tacagcacct tacggtcatt aatcagtcgc
     9301 atcagcaata tctgcaaaaa gacctgccgg atttatatgt cgatctctct accgtcggcg
     9361 aaggttatgc ggcggatcac ttggcacgct tgatggagca ggaagggatt tcccgctatc
     9421 tggtgtcggt gggcggcgcg ctgaacagcc gtggtatcaa cggtgaaggc ctgccgtggc
     9481 gggtagcgat tcaaaaacca accgataaag aaaacgcagt tcaggctgtg gtggatatca
     9541 acggtcatgg gattagcacc tctggcagct accgtaacta ttacgaactg gacggcaaac
     9601 gtctttccca tgttatcgat ccgcaaaccg ggcgtcccat cgaacacaat ctggtatccg
     9661 tgacggtgat tgctccgacg gcgctggaag ccgatgcctg ggatactggc ttgatggtac
     9721 tcgggccgga gaaagccaaa gaggttgttc gccgggaagg gctggcggtt tatatgatca
     9781 ccaaagaagg cgatagcttt aaaacctgga tgtcaccaca gtttaaaagc ttccttgtca
     9841 gcgaaaaaaa ttaaagcgca agattgttgg tttttgcgtg atggtgaccg ggcagactaa
     9901 aggctatcct taaccaggga gctgattatg aaaaacgcca catgcttaac tgacgatcaa
     9961 cgctggcaat ctgtcttagc ccgcgacccg aatgccgacg gcgaattcgt tttcgccgtg
    10021 cgcaccacag gcatcttttg ccgtccgtct tgccgcgcca gacatgcctt gcgggaaaat
    10081 gtctccttct acgcaaatgc cagcgaggca ctcgccgccg gatttcgccc ctgcaaacgc
    10141 tgccaacctg ataaagccaa tgcccagcaa catcggttgg ataaaatcac ccacgcgtgt
    10201 cgactgctgg aacaggaaac gcctgtaacg ttggaagcct tagccgacca ggtggcgatg
    10261 agtccatttc atctacatcg gttgtttaaa gcgactaccg gaatgacgcc taaagcctgg
    10321 caacaggcct ggcgcgctcg ccgtttgcgc gaatcgctgg cgaaagggga gagcgtgacg
    10381 acgtctattc ttaacgccgg attccccgac agcagcagtt actaccgcaa agccgatgaa
    10441 acgctgggca tgacggctaa acaattccgt cacggtggcg aaaatctggc ggtgcgttac
    10501 gcgctggctg attgtgcgct gggtcgttgc ccggtggcag aaagcgagcg ggggatttgc
    10561 gcgatattgc tgggcgatga tgacgcgaca ttaatcagcg agttgcagca gatgtttccc
    10621 gctgccgaca acgcgcctgc cgatctgacg tttcagcaac atgtgcgtga agtgatcgcc
    10681 agcctcaatc aacgcgatac gccgctgacg ttaccgctgg atattcgcgg cactgctttt
    10741 cagcaacaag tctggcaggc actgcgcacg ataccttgcg gtgaaaccgt actggctaac
    10801 gccatcggca aaccgaaagc ggtacgggcc gttgccagcg cctgtgccgc caacaagctg
    10861 gctatcgtaa taccctgtca tcgagtggtc ggtggtgatg gcacactttc cggttaccgc
    10921 tggggcgtgt ctcgtaaagc gcaactgctg cgccgcgaag ctgaaaatga ggagaggtaa
    10981 tgttggatct gtttgccgat gctgaaccgt ggcaagagcc actggcggct ggtgcggtaa
    11041 ttttacggcg ttttgctttt aacgctgcgg agcaactgat ccgcgatatt aatgacgttg
    11101 ccagccagtc gccgtttcgc cagatggtca cccccggggg atataccatg tcggtggcga
    11161 tgaccaactg tgggcatctg ggctggacga tccatcggca aggttatctc tattcgccca
    11221 ttgatccgca aacaaataaa ccgtggcccg ccatgccaca aagctttcat gatttatgtc
    11281 aacgtgcggc tacggcggcg ggctatccag atttccagcc agatgcttgt cttatcaacc
    11341 gctacgctcc tggcgcgaaa ctgtcgctgc atcaggataa agacgaaccg gatctgcgcg
    11401 cgccaattgt ttctgtttct ctgggcttac ccgcgatttt tcaatttggt ggcctgaaac
    11461 gtaatgatcc gctcaaacgt ttgttgttgg aacatggcga tgtggtggta tggggcggtg
    11521 aatcgcggct gttttatcac ggtattcaac cgttgaaagc ggggttttat ccactcacca
    11581 ccgactgccg ctacaacctg acattccgtc aggcaggtaa aaaagaataa aaataagaat
    11641 tattattgct gtgcgcgcga agatgtttaa actgcgggct gtaattcatt gtccgggttt
    11701 tctgcatgga acttcttgta cttgtctggc ggcagtatcg ctggccattt atcagtgtga
    11761 tggcgctaag cctcgccagt gcggcattag gcattggctt aattgctttt atcaatcagc
    11821 gccttatcga aatggcggat accagtctgc tggtattgcc ggagtttctg ggattattgc
    11881 tgctgttgat ggcagtcact ctcggatcgc aactggcgct caccactttg gggcatcact
    11941 tcgtttaccg actgcgtagt gaatttatca agcggattct ggatacccac gtcgagcgca
    12001 ttgaacaact cggtagcgcc tcgttgttgg cgggattaac cagcgatgtg cgcaatatca
    12061 ccattgcttt tgtgcgtctt ccggaactgg tgcaggggat cattctcact atcggttccg
    12121 cggcgtatct gtggatgctg tcgggcaaaa tgttgctggt aacggctatc tggatggcga
    12181 tcaccatctg gggtggtttt gtactggtgg cgcgggtgta caaacatatg gcgaccctac
    12241 gtgaaaccga agacaagctg tacacggatt ttcaaactgt acttgaaggg cgcaaagagc
    12301 tgactctgaa ccgggaacgc gccgagtatg tgtttaacaa cctctacatc cccgatgcgc
    12361 aagagtatcg ccatcatatt attcgcgcag acaccttcca tcttagtgcc gtgaactggt
    12421 caaacatcat gatgctgggc gctatcggcc tggtgttctg gatggcgaac agtctcggtt
    12481 gggctgatac caacgttgct gcgacctatt cgttgacgct tttattcctg cgtacgccgc
    12541 tgctttcggc ggttggcgca ttgccgacgc tgctgatggc gcaggtggcg tttaacaagc
    12601 tgaacaaatt cgcgctcgcg cctttcaaag cagagtttcc gcgcccgcag gcgtttccca
    12661 actggcaaac gctggagctg cgtaatgtga cgtttagcta tcaggataac gcgttttccg
    12721 ttggtccgat taatctcacc atcaaacgtg gcgagctact gtttctgatt ggtggtaacg
    12781 gtagtggaaa atcgacgctg gcgatgttgt tgacgggctt gtatcagcca caaagcggcg
    12841 aaatcttgct ggatggcaaa cctgtcagcg gcgaacaacc ggaagattat cgcaaactgt
    12901 tttcggcagt gtttaccgat gtctggcttt ttgatcaact gctggggccg gagggtaaac
    12961 ccgctaaccc gcaactggtt gagaagtggc tggcgcagct gaaaatggct cataagcttg
    13021 agttaagcaa cgggcgtatt gttaacctga agttatcaaa agggcagaaa aaacgcgtgg
    13081 cgctgttgct ggcgctggca gaagaacgcg atattatcct gctggatgaa tgggcggcgg
    13141 atcaggatcc acacttccgt cgtgagtttt atcaggtgtt gctgccgctg atgcaggaga
    13201 tgggtaaaac tattttcgct atcagtcatg atgatcatta ctttatccac gccgaccgcc
    13261 tgctggaaat gcgcaatggg caacttagcg agctgacggg cgaagagcgc gatgccgctt
    13321 cgcgtgatgc cgttgcccgg acggcataat attacgacgc tgacctggcg gactgctgcc
    13381 gtcaggtcaa tatgctaaaa atcccttcat ttctaataac cctataatta atcaacgaaa
    13441 ttataatgtt tctaaaatta gaatataatt tataaacatt atttaaatgt tgttacttaa
    13501 gtgttacctt tgatgccgcg caaatctcac ctgcaataaa gcgactaaaa gtaaggcatt
    13561 aacaagatga aaaaagtgac tgccatgctc ttctcgatgg ccgtggggct taatgccgtt
    13621 tcgatggcgg caaaagcgaa agcgtccgag gagcaggaaa ctgatgtact gttgattggc
    13681 ggcggcatta tgagcgccac gttggggacc tatttacgcg agctggagcc tgaatggtcg
    13741 atgaccatgc tggagcgcct ggagggtgtc gcgcaggaga gttcgaacgg ctggaataac
    13801 gccggaaccg ggcattctgc actgatggaa ctgaactaca ccccgcaaaa cgccgatggc
    13861 agtatcagta ttgaaaaagc agtcgccatt aacgaagcat ttcagatttc ccgccagttc
    13921 tgggcgcacc aggtcgagcg cggcgtgctg cgtactccgc gttcatttat caataccgtt
    13981 ccgcatatga gctttgtctg gggcgaggat aacgttaatt tcctgcgcgc ccgttacgcc
    14041 gcgttgcaac aaagctcgct gtttcgcggt atgcgttact ctgaagatca cgcgcagatc
    14101 aaagagtggg caccgttagt gatggaaggg cgcgatccgc aacagaaagt ggcagccacg
    14161 cgtacggaaa ttggtaccga tgtgaactac ggcgagatca cccgccagtt aattgcttcc
    14221 ttgcagaaga aatctaactt ctcgctgcaa ctcagcagcg aagtccgcgc cctaaagcgt
    14281 aatgacgata acacctggac cgttaccgtt gccgatctga aaaatggcac tgcacagaac
    14341 attcgtgcca aatttgtctt tatcggcgcg ggcggtgcgg cgctgaagct gttacaggaa
    14401 tcggggattc cggaagcgaa agactacgcc ggtttcccgg tgggcggaca gttccttgtt
    14461 tcggaaaacc cggacgtggt taatcaccat ctggcgaagg tttacggtaa agcatccgtt
    14521 ggcgcaccac cgatgtcggt tccgcatatc gatacccgcg ttctggacgg taaacgcgta
    14581 gtgctgtttg ggccatttgc caccttctca accaaattcc tcaaaaacgg ttcattgtgg
    14641 gatctgatga gttccaccac cacctctaac gtgatgccga tgatgcacgt cgggctggat
    14701 aatttcgatc tggtgaaata tctggtgagt caggtgatgt tgagtgaaga ggatcgtttt
    14761 gaagcgttga aagagtacta tccgcaggcg aaaaaagagg actggcgttt gtggcaagcg
    14821 gggcagcgtg tgcagattat caagcgtgat gccgataaag gtggtgtact gcgtctgggt
    14881 actgaagtgg tcagtgacca gcaagggacc attgccgcgc tcctgggagc atcgccaggg
    14941 gcgtcaaccg ccgcgccgat tatgttggat ctgctggaaa aagtatttgg cgatcgcgtt
    15001 tccagcccgc aatggcaggc tacgttgaaa gcgatcgttc cgtcttatgg acgcaagctg
    15061 aacggtgatg tagcggcaac agaacgcgag ttgcagtaca ccagcgaagt gctggggctg
    15121 aaatacgaca agccgcaggc agcagatagt acgccaaaac cgcagttgaa accgcaaccc
    15181 gttcaaaaag aagtggcgga tattgcgttg taatgatgtg ccacatccgg catggtatgc
    15241 cggatgtggc gtatactgat aagacgcgcc agcgtcgcat caggcaaccg gctcgggcgt
    15301 tagatgttgg atgcggcgta accgccttat ccaaccaacg gggtgccgta cttcacaaca
    15361 gtttagcgaa cttctgcgtt gtcaattttc tcttccgcct tccagacgcg gtatttcaca
    15421 tctacattgt ctggcgtata caccacgatc ggcagcttgc tgttgtaacg cagcattcca
    15481 gcatcgccca gatacgcggt gacaaatttc ttctctttct tgccatccgg gcaggccatc
    15541 atcgtggaaa ccggcgagct aactttatca aagacataat aatcatagcc ccagccttcc
    15601 agcgttttgc tttccagctt cccgccgaga cgatgcaaat tgcaatcgac ttccagcgtc
    15661 tgaccgatta acagttctac tttcagggta gattcatctt cttgcggggt taactgaatc
    15721 acctgacgct tcatcccttt ttcagcttgt ggataaggcg cgattttttc cagtggctgg
    15781 acgctttctg ccgcccaggc ggaagtggta gcgaaagcgg caaacaatac tgcaggtaga
    15841 atggtcttca tttttttgct ttccttcttc atttataggt tttcaccgat gatgtcgctg
    15901 aaggttaaca tattcataag atttattaat ttgctttatg aataacttaa agaatattca
    15961 gacaatttta cctgctcttt tagtgttaaa ttctgatgag agaacaaacc agcagaaata
    16021 tctctggtga attaatcgct atataaatat gtttataacc atttgaaatg tgagcaaaag
    16081 cccgtttttg ccacgcttcc cgccactctt ttgatcctgc tacaggtttt accccgatcg
    16141 gggtatgcat ctttgacaca tcctttaata tcttagcggt tataaaaatg gcttattaat
    16201 tatgcggttt atttggtcgc tctcaatttt cagagcgcgt taatgatgga aggtcaatgt
    16261 gaagattgat gcatcccgtc ggggcatact cactggtcgc tggcgcaaag ccagtaacgg
    16321 tatccgtccg ccctggtccg gtgatgaatc tcattttctg acccattgca cccgttgcga
    16381 cgcctgtatt aatgcttgtg aaaacaacat tctgcaacgc ggcgcgggcg gctatccgag
    16441 cgttaatttc aaaaataacg agtgcagctt ctgttacgct tgcgcgcagg cttgccccga
    16501 atcgctattt tctccgcgcc acaccagggc ctgggatttg cagttcacca ttggggacgc
    16561 gtgcctggcg tatcagtcag ttgaatgccg ccgctgtcag gatagctgtg agccaatggc
    16621 aattatcttt cgcccgacgc tctccgggat ctaccagccg caacttaata gccaactttg
    16681 caacggatgt ggagcatgtg cagccagctg tccggtatca gccataactg cggagtatct
    16741 ccatgcacac taactggcaa gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt
    16801 cagacatcag cacccaactg aacgcctttc ccggctgtga agttgctgtc agcgacgcgc
    16861 cgagcggtca gttgattgtg gtggtggaag cagaagacag cgaaacgctg atccaaacca
    16921 ttgagtcagt acgcaacgta gagggcgtgc tggcggtgtc gctggtttat caccagcagg
    16981 aagagcaagg tgaggaaaca ccatgaaact cagtcgtcgt agctttatga aagctaacgc
    17041 cgttgcggcc gctgcggcgg ctgccggtct cagcgtgccg ggcgttgccc gcgccgttgt
    17101 tggtcagcag gaagccatca aatgggataa agcgccgtgc cgtttctgcg gtactggttg
    17161 cggcgttctg gtcggaacgc agcagggacg tgtggtggcc tgtcagggcg acccagacgc
    17221 accggttaac cgtggcctga actgcattaa gggctatttc ctgcccaaga tcatgtacgg
    17281 taaagaccgt ttgacgcagc cgctgctgcg tatgaaaaac ggtaaatatg acaaagaagg
    17341 cgaatttacc ctaatcacct gggatcaggc cttcgatgtg atggaagaga aattcaaaac
    17401 cgccctgaaa gaaaaagggc cggaatcgat cggtatgttc ggttctggtc agtggactat
    17461 ctgggaaggt tatgccgcgt ccaagctgtt taaagcgggc ttccgttcga acaacatcga
    17521 cccgaacgcg cgtcactgta tggcgtcggc agtagttggc tttatgcgta cctttggtat
    17581 ggatgagccg atgggctgct atgacgacat cgagcaggct gacgcgtttg tgctgtgggg
    17641 cgcaaacatg gcggagatgc acccgatcct ctggtcgcgc atcactaacc gtcgtctctc
    17701 taatcagaac gtcaccgtgg cggtgctttc tacctaccag catcgtagct tcgagctggc
    17761 ggataacggc atcatcttta cgccacagtc tgacctggtg atcctgaact acatcgccaa
    17821 ctatatcatt caaaacaatg cgataaatca ggacttcttc agcaaacacg ttaacctgcg
    17881 caaaggggcg acggacattg gctacggttt acgtccgacc catccgctgg aaaaagcagc
    17941 gaagaatcca ggttctgacg cctccgaacc gatgagcttt gaagattaca aagccttcgt
    18001 tgccgagtat acgctggaaa aaactgccga aatgaccggc gtgccgaaag accagttaga
    18061 acaactggcg cagctgtatg ccgatccgaa caagaaagtc atctcctact ggacgatggg
    18121 cttcaaccag catactcgtg gcgtgtgggc taacaacctg gtctacaacc tgcacctgct
    18181 gaccggcaaa atttcccagc cgggttgcgg tccgttctcc ctgaccgggc aaccttctgc
    18241 gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt ctgcctgcgg acatggtggt
    18301 gaccaacgag aaacaccgtg atatctgcga gaagaagtgg aatatcccga gcggcaccat
    18361 tccggcgaaa atcggtctgc acgcggtggc acaagaccgt gcgctgaaag acggcaaact
    18421 gaatgtttac tggaccatgt gtaccaacaa catgcaggcc gggccgaaca tcaatgaaga
    18481 gcgtatgccg ggctggcgcg atccgcgcaa cttcatcatc gtctccgatc cgtatccgac
    18541 agtcagtgcg ctggccgccg acttgatcct gccgaccgca atgtgggtag agaaagaggg
    18601 cgcttacggt aacgccgaac gccgtactca gttctggcgt caacaggtac aggcaccggg
    18661 cgaagcgaaa tcggatctct ggcagttagt ccagttctcc cgccgcttca aaactgaaga
    18721 agtatggccg gaagatctgc tggcgaagaa accggaactg cgtggcaaaa cgctgtacga
    18781 agtgctgtat gccacaccgg aagtgagcaa attcccggta tccgaactgg cggaagatca
    18841 gctgaacgat gaatcccgcg agctgggctt ctatctgcaa aaagggctgt tcgaagagta
    18901 cgcatggttt ggtcgcggtc acggtcacga tctggcaccg ttcgatgact accacaaagc
    18961 gcgcggtctg cgctggccgg tggtgaacgg taaagaaacg cagtggcgtt acagcgaagg
    19021 taacgacccg tacgtgaaag cgggcgaagg ctataagttc tacggcaaac cggatggcaa
    19081 agcggtgatc ttcgcactgc cattcgaacc ggcggcggaa gcaccggatg aagagtacga
    19141 cctgtggctc tctaccggac gcgttctgga gcactggcac accggcagta tgactcgccg
    19201 tgtaccagaa ctgcaccgcg ccttcccgga agcggtcctg tttattcacc cgctggatgc
    19261 gaaagcgcgc gatctgcgtc gtggcgacaa agtgaaagtg gtttctcgcc gtggcgaagt
    19321 gatctcgatt gtcgaaacgc gcggtcgtaa ccgtccgccg cagggcctgg tgtacatgcc
    19381 gttcttcgac gccgcacagc tggttaacaa actaacgcag gacgcgaccg atccgctctc
    19441 gaaagagacg gacttcaaga agtgcgcagt caaactggag aaggtgtaag ccattatgtc
    19501 ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg cgcgatgttg ttcgcacagc
    19561 aggcgggctg gctgccgtgg gtgtggcgct ggggttacaa cagcaaaccg cacgcgcatc
    19621 tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac gcctttgcca gtgcctgtgt
    19681 gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc ttaaaactgg cgacgctggc
    19741 ctctggtctg tcggcgggca cgccatattt tgtcgcacgg gatattcctt gcgaaatgtg
    19801 tgaggacatt ccgtgcgcca aagtgtgccc gagcggtgcg ctggatcgtg agatcgaatc
    19861 gatcgacgac gcgcggatgg ggctggcggt actggtggac caggaaaact gtctcaactt
    19921 tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg aaaattgatg aggccatcac
    19981 cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc cgctttctgc cgacggttca
    20041 cagagacgcc tgtaccggtt gcggtaagtg cgaaaaagtg tgcgtgctgg aacaaccggc
    20101 aatcaaggtg ttaccgctgt cactggctaa aggggagtta ggtcaccatt accgcttcgg
    20161 ctggctggag gggaacaatg gcaaatcgta aacgtgacgc cgggcgcgag gcgctggaga
    20221 aaaaaggctg gtggcgcagt caccgttggc tggtgttacg tcgcctttgt cagttcttcg
    20281 tgctggggat gtttttgagc ggtccgtggt ttggtgtgtg gatcttacac ggtaactaca
    20341 gcagtagcct gttattcgac accgttccgc tgaccgatcc gctgatgacg ctgcaaagtc
    20401 ttgccagcgg tcatctgcct gccacggtgg cgttgaccgg tgcggtgatt atcaccgtgc
    20461 tctacgccct ggcaggaaag cgattatttt gcagctgggt ctgcccgctg aatccgatta
    20521 ccgacttagc gaactggtta cgcaggcgct ttgacctcaa tcagtctgcg acgatccctc
    20581 gccacattcg ctacgtgctg ttggtggtca ttctgctggg atcggcctta actggcacgc
    20641 tcatctggga atggatcaac cccgtatctc tgatggggcg cagcctggta atgggcttcg
    20701 gcagcggcgc gctgcttatt ctcgcgctgt ttttatttga tctactggtc gttgaacacg
    20761 gctggtgcgg gcacatttgt ccggttggcg cgctgtatgg cgtgctgggt agcaaaggtg
    20821 tgattaccgt tgcggccacc gatcggcaga aatgtaaccg ctgtatggat tgttttcatg
    20881 tttgcccgga accgcgtgtg ctacgtgccc cggtgctgga tgaacaaagc ccggtgcagg
    20941 tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga tgtctgttct gaggatgtat
    21001 ttacaataac tacacgatgg agttcgggag cgaaatcatg aaaagccatg acctgaagaa
    21061 agcgctgtgt caatggacgg cgatgctggc cctggtggta agcggcgcgg tttgggcggc
    21121 taatggcgtc gattttagcc agtcgccgga agtatccggg acacaggaag gggccattcg
    21181 gatgccaaaa gagcaggaac ggatgccgct gaactatgtg aatcagccgc caatgatccc
    21241 gcatagcgtt gaaggttatc aggtaacgac caataccaac cgctgcttgc agtgccacgg
    21301 tgtcgaaagt tatcgcacca ctggcgcgcc gcgcattagt cctactcact ttatggacag
    21361 cgacggcaaa gtgggcgcgg aagtggctcc gcgtcgttat ttctgtctgc aatgtcacgt
    21421 accgcaggcc gataccgcgc caatcgtggg gaataccttt accccatcaa aaggttacgg
    21481 gaaataagag gttattatgg gaaattctga ccgtaagcct ggtctgatta agcgcctgtg
    21541 gaaatggtgg cgtaccccca gccgtctggc gctggggacg ctgctgttga tcggttttgt
    21601 tggcggcatc gtcttctggg gtggctttaa caccgggatg gaaaaagcca ataccgaaga
    21661 gttctgcatt agctgccacg aaatgcgcaa cacggtgtat caggaataca tggattccgt
    21721 gcactacaac aaccgtagcg gcgtccgtgc gacctgtccg gattgtcacg ttccgcacga
    21781 gtttgtgccg aagatgatac gcaagctcaa agcaagtaaa gagctgtatg gtaaaatttt
    21841 tggcgttatt gacacgccgc agaaatttga agctcatcgt ctgacgatgg cacagaatga
    21901 gtggcggcgc atgaaggaca ataactcgca ggagtgccgt aactgtcaca acttcgagta
    21961 tatggataca accgcccaga aatcggttgc cgcgaagatg catgaccagg cggtgaaaga
    22021 tgggcaaacc tgtattgatt gccataaagg gatagcgcac aagctgcccg atatgcgtga
    22081 agtcgagcca ggtttttaac agggttattg cgtgggtatg cttgaagcca gagagttact
    22141 ttgtgagcgg gatgaacgaa ccttatttag tggcttgtca tttacgctga acgcaggaga
    22201 gtgggtacaa atcaccggta gcaacggcgc ggggaagaca acgcttctcc gtttgctgac
    22261 ggggttgtct cgccctgacg caggcgaggt tctctggcaa gggcagccct tgcatcaggt
    22321 acgcgacagc taccatcaaa acctgttatg gataggccat cagccgggga tcaaaacccg
    22381 gctgacggcg ttagaaaatc tgcactttta tcatcgcgat ggcgataccg cacaatgtct
    22441 ggaagccctg gcgcaggccg ggcttgccgg attcgaagat attcctgtaa atcagctctc
    22501 ggccgggcaa caacgccgcg tcgctttagc gcgtctgtgg ctgacccgtg ccacgttatg
    22561 gatcctcgac gagcctttta ccgcgattga cgttaacggc gtcgatcgtc tgacccagcg
    22621 tatggcgcag catacggagc agggggggat tgtgattctg actacccacc agccgctcaa
    22681 cgttgctgaa agtaaaattc gccgcatttc actgacgcaa acgagggccg catgatgttc
    22741 tggcgcattt tccgtcttga gctgcgtgta gcgtttcgcc atagcgccga aatcgccaac
    22801 ccgctgtggt tcttcctgat tgtaattacc ctttttccgc tcagtatcgg tccggagccg
    22861 caactgctgg cgcgtattgc accgggcatt atctgggttg ctgcgctgct ttcatccttg
    22921 ctggcgctgg aacgactgtt ccgtgacgat ttgcaggacg gcagtcttga acaattgatg
    22981 ttgttgccgt tacccttgcc cgccgttgtg ctggcgaagg tgatggcgca ctggatggta
    23041 accggtctgc cgttactcat cctttcgcca ctggtagcaa tgctactggg aatggatgtt
    23101 tatggctggc aagtgatggc gctgacgctg ctgctgggaa cgcctacgct tggctttctc
    23161 ggtgcaccgg gtgtggcgtt gacagtggga cttaagcgcg gtggtgtgct gctcagcata
    23221 ctggtgttac cgctgactat cccattactc atctttgcca ccgccgcgat ggacgcggct
    23281 tccatgcatt tgcccgttga cgggtatctg gcaattttag gcgcgttgct ggcaggcacc
    23341 gcgacattaa gtccttttgc gacggcggca gcgttacgaa tcagcattca ataacgctgg
    23401 attactgatt tttgttcgag tctggtatcg aaactatgtg gaaaacactg catcaactgg
    23461 cgatcccacc acggctgtat caaatctgtg gctggtttat accgtggctg gcaattgcca
    23521 gtgtggtcgt gcttaccgtc ggctggatct ggggattcgg ctttgctccg gctgattatc
    23581 agcagggaaa tagctaccgc attatctacc tgcatgtgcc tgcggcgatc tggtcgatgg
    23641 gtatttatgc atcaatggcg gtggcggcgt ttattggcct tgtctggcag atgaaaatgg
    23701 ccaacctggc ggtggcggcg atggccccca ttggtgccgt gtttaccttt attgccctgg
    23761 ttaccggctc tgcatgggga aaaccgatgt ggggcacctg gtgggtatgg gatgcacgtc
    23821 tgacttctga actggtgctg ctgtttttgt atgtgggtgt gattgccctg tggcacgcct
    23881 tcgacgaccg ccgtctggcg ggccgtgcgg caggtatcct ggtgctgatt ggcgtggtga
    23941 atctgccgat tattcattac tccgtggagt ggtggaacac cctgcatcag ggatcaacgc
    24001 ggatgcagca aagtatcgat ccggcgatgc gttcgccgct gcgctggtcg atttttggct
    24061 tcctgctcct gtctgccacg ctgacgctga tgcggatgcg taatttgatt ttgctgatgg
    24121 aaaaacgccg tccgtgggtg agtgaactga tactgaaaag aggccgtaaa tgacccttgc
    24181 atttgcttcc tggaatgaat ttttcgcaat gggcggttac gccttttttg tctggctggc
    24241 ggtggtgatg accgttattc cgctggtggt tttggtcgtg cactcggtga tgcagcatcg
    24301 cgcaattctg tgtggtgtgg agcaacagcg ggcgcgtgag gcgcgtttac gtgctgcgca
    24361 acagcaggag gctgcatgaa tattcgccgt aaaaaccgct tgtggattgc ctgtgctgtg
    24421 ttggcaggtt tggcgctgac catcggtctg gtgctgtatg cgctgcgctc gaatatcgat
    24481 ctcttttata cgccggggga aattctctac ggcaagcgtg aaactcagca aatgccggaa
    24541 gtcggtcagc gtctgcgcgt tggcgggatg gtgatgccgg gtagtgtgca gcgcgatccc
    24601 aattcgctga aagtgacctt caccatttac gacgctgaag gctcagtgga tgtctcttac
    24661 gaaggcattt tgccggatct gttccgtgaa gggcagggcg ttgtggtgca gggcgagctg
    24721 gaaaaaggca atcatatcct cgcgaaagaa gtgctggcga aacacgacga aaactacacg
    24781 ccgccagaag ttgagaaagc gatggaagct aaccaccgtc gcccggcgag tgtttataag
    24841 gacccagcat catgatgccg gaaattggaa acgggctgct gtgcctggcg ctgggaattg
    24901 cgctgctgct gtccgtgtat ccgctatggg gcgtggcgcg cggagatgcg cgcatgatgg
    24961 cgtcttcccg cttgtttgcc tggatgctgt ttatgtctgt ggctggcgca tttctggtgc
    25021 tggtcaatgc tttcgtggtc aacgacttta ccgtcaccta tgttgccagc aactccaata
    25081 cccagcttcc ggtgtggtat cgcgtggcgg ctacctgggg cgcgcatgaa ggttcgcttc
    25141 tgctgtgggt gctgctgatg agcggctgga cctttgcggt ggcgattttt agtcagcgta
    25201 ttccgctgga tattgtggcc cgtgtactgg cgataatggg gatggtcagc gtcggctttt
    25261 tgctgttcat tctctttacc tctaacccgt tctcacgcac gctgccgaac ttcccaattg
    25321 aagggcgcga tcttaacccg ctgttacagg atccggggct gatcttccat ccgcctctgc
    25381 tttatatggg gtacgtgggt ttctcggtgg cgtttgcttt tgccattgcc attgcttctt
    25441 tgctgagcgg gcgtctggac agcacttatg cgcgttttac tcgcccgtgg acgctggcgg
    25501 cgtggatttt cctgacgctc ggcattgtgc tcggttccgc atgggcctat tacgaactcg
    25561 gctggggggg ctggtggttc tgggatccgg tagaaaacgc ctcgtttatg ccgtggctgg
    25621 tggggactgc gctgatgcac tcactggcgg tcactgaaca acgcgccagc ttcaaagcgt
    25681 ggacattact gctggcaatc agtgccttct cgttgtgtct gctggggact ttcctggtgc
    25741 gttccggcgt gctggtatcg gtacacgcgt ttgcctctga tccggcacgc ggtatgttta
    25801 tcctcgcctt tatggtactg gtgattggtg gttcgctgct gctgtttgcc gcgcgtggac
    25861 acaaagttcg ctcacgcgta aacaatgcgc tgtggtcgcg ggaatctctg ctgttagcga
    25921 acaacgtttt gttggtcgcc gcgatgctgg tggtattact ggggacgctg ctgccgctgg
    25981 tgcacaagca actgggactg ggcagtattt cgattggcga accgttcttc aacaccatgt
    26041 ttacctggct gatggtgccg tttgcgctgc tgcttggtgt cggtcctctg gtgcgctggg
    26101 ggcgcgatcg cccacgtaaa atccgcaatt tattgattat cgccttcatc tcaacgctgg
    26161 tgctgtcgct gctgctgccg tggctgttcg aaagcaaagt tgtggcgatg acggtgctcg
    26221 gcctggcaat ggcctgctgg attgctgtgc tggcaattgc ggaagctgcg ctgcgtattt
    26281 cacgtggcac gaaaaccacc ttcagttatt ggggaatggt ggcggctcac ctggggctgg
    26341 cagtgacaat tgttggtatt gcctttagcc agaactatag cgttgagcgt gatgtgcgca
    26401 tgaagtccgg cgatagcgtc gatattcatg aatatcgctt caccttccgt gatgtcaaag
    26461 aggtgactgg cccgaactgg cgcggcggtg tggcgactat tggcgtaacg cgagagggta
    26521 aaccggaaac ggtgctgtat gcggaaaaac gttattacaa cactgccggg tcgatgatga
    26581 ccgaagcggc gattgacggc ggcatcacgc gtgacctgta cgccgcgctc ggtgaagagc
    26641 tggaaaacgg cgcgtgggcc gtgcgtcttt actacaaacc gtttgttcgc tggatttggg
    26701 cgggcgggct gatgatggcg ttgggcggac tgctgtgtct gtttgatcct cgctatcgta
    26761 agcgcgtgag tccgcaaaaa actgcgccgg aggctgtatg aagcgcaaag tattgttaat
    26821 tccgttgatt atcttcctgg cgattgccgc ggcgctgctg tggcagctgg cgcgtaatgc
    26881 cgaaggggat gatccgacca atctggaatc ggcgctcatt ggcaagccag tgccgaagtt
    26941 tcgcctcgaa tcactggaca atccggggca gttttatcag gcggatgtgc tgactcaggg
    27001 caaacctgtg ctgcttaacg tttgggctac ctggtgtccg acctgccgtg cggaacatca
    27061 atatctgaat cagctttctg cgcagggcat tcgcgtggtc ggcatgaact ataaagacga
    27121 tcgccagaag gcaataagct ggctgaaaga gctgggcaat ccttacgcgc taagcctgtt
    27181 tgatggcgat ggcatgttag ggctggatct cggtgtctat ggcgcgccag aaacgttcct
    27241 catcgacggc aacggcatca ttcgctatcg ccatgcgggc gatctcaatc ctcgcgtctg
    27301 ggaagaagag atcaagccgc tgtgggagaa atacagtaag gaggccgcac aatgaggttt
    27361 ttattgggcg tgctgatgct gatgatctcc ggctcagcgc tggcgaccat cgacgtgttg
    27421 cagtttaagg atgaagcgca ggagcagcag ttccgccaac tcactgaaga actacgctgc
    27481 ccgaaatgcc agaacaacag cattgccgat tccaactcga tgattgccac cgacctgcgc
    27541 cagaaagtgt atgaactgat gcaggaaggt aaaagtaaaa aagagattgt cgattatatg
    27601 gtggcgcgtt acggcaactt cgtcacttac gatccgccgt taacgccgct gaccgtgctg
    27661 ctgtgggtgc ttccggtagt ggctattggc attggcggtt gggtcattta cgcccgttcg
    27721 cggcgtcggg tacgcgtggt gccggacgcg tttcctgaac aaagcgtgcc ggaaggtaag
    27781 cgtgccggat atattgttta tctgccgggt attgtggtgg cgttaattgt ggctggcgtc
    27841 agctactacc agactggcaa ttatcagcag gtgaaaatct ggcagcaggc cacggcacag
    27901 gctccggcgt tgctggacag ggcgctggat ccgaaagccg atccgctcaa cgaagaagag
    27961 atgtcgcgtc ttgcgctggg gatgcgtact caactgcaaa aaaatccggg agatatagaa
    28021 ggctggatta tgttgggccg cgttggcatg gcgctgggta acgccagtat cgccaccgat
    28081 gcatacgcta ctgcgtatcg cctcgatccg aaaaacagtg atgccgcact gggttatgct
    28141 gaagcgttga cacgttcatc tgatcccaac gacaaccgcc tcggtggtga actgctgcgc
    28201 cagttggtga gaacggacca tagcaatatc cgtgtgttaa gcatgtatgc gtttaatgcc
    28261 tttgagcagc agcgatttgg cgaagccgtt gccgcgtggg agatgatgtt gaaactctta
    28321 tctgccaacg atactcgccg tgcggtgatt gaacgtagta tcgcgcaggc gatgcaacat
    28381 ttgtcgccgc aggagagtaa ataaaaagag gcccgatggt gatgccatcg ggctattttt
    28441 attgtgcccc gcgttgttgc aggaacagaa tggtggctgc cacgcgtgag cggacattga
    28501 gtttgcgcag cagattgcga atatgtactt ttactgtctg ctctgaaata ttcaacaccg
    28561 aggcaatctg tttatttgac agcccctgtg ccagctcgtg cagaacatcc agctcgcgct
    28621 ccgtcagcac gctgaaggga tcttcttccg cgccaaacat ttcacgttca cgtaagtact
    28681 gattgacgcg ttcgctaaag actttgctgc ctttcgctcc agcacgaatt gcttccagca
    28741 atacttccgg gtcgctgtct ttcaacagat aaccgtctgc gcctgcgtct atcagcgcaa
    28801 agacatcgct ggaggcatct gatacggtca ggataataat ttgcgcggta acgccatccc
    28861 tgcgcaaggc attgagggta tccaggccac tcataccttt catattgaga tccagcaaga
    28921 tcacgtcgat atccagtcta tttgccagat cgatcgcgct cgcgccgtcg cccgcttcgg
    28981 cgaccacttc aaagctagga tcaagctcca gtaactgacg aacaccgcgt cgcataagtg
    29041 gatgatcatc cacaatcatc acctgaaaag gcgttgcttc aggcatagta gtctcctggg
    29101 gttttattag aatcattatg tttgagcgta gcgttggtaa cgctaccagg cgcgtgaatg
    29161 ttaccccatt ttatgtatgg acatgtagtg aaaactacaa ccttgtcgtt cgctttggca
    29221 tcattttcat tataaagacg gaattttgca tca
//
LOCUS       DAJTNN010000031        28862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTNN010000031 DAJTNN010000000
VERSION     DAJTNN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.031"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            163..606
                     /locus_tag="OO150_001238"
     CDS             163..606
                     /locus_tag="OO150_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5515448.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLTDVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            842..1660
                     /gene="ybhA"
                     /locus_tag="OO150_001239"
     CDS             842..1660
                     /gene="ybhA"
                     /locus_tag="OO150_001239"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5515449.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1661..2719)
                     /gene="modC"
                     /locus_tag="OO150_001240"
     CDS             complement(1661..2719)
                     /gene="modC"
                     /locus_tag="OO150_001240"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5515450.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2722..3411)
                     /gene="modB"
                     /locus_tag="OO150_001241"
     CDS             complement(2722..3411)
                     /gene="modB"
                     /locus_tag="OO150_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5515451.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3411..4184)
                     /gene="modA"
                     /locus_tag="OO150_001242"
     CDS             complement(3411..4184)
                     /gene="modA"
                     /locus_tag="OO150_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5515452.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4350..4499)
                     /gene="acrZ"
                     /locus_tag="OO150_001243"
     CDS             complement(4350..4499)
                     /gene="acrZ"
                     /locus_tag="OO150_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5515453.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4628..5416
                     /gene="modE"
                     /locus_tag="OO150_001244"
     CDS             4628..5416
                     /gene="modE"
                     /locus_tag="OO150_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5515454.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5484..6956
                     /gene="modF"
                     /locus_tag="OO150_001245"
     CDS             5484..6956
                     /gene="modF"
                     /locus_tag="OO150_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5515455.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKILLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7217..8233
                     /gene="galE"
                     /locus_tag="OO150_001246"
     CDS             7217..8233
                     /gene="galE"
                     /locus_tag="OO150_001246"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5515456.1"
                     /translation="MRVLLTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8243..9289
                     /gene="galT"
                     /locus_tag="OO150_001247"
     CDS             8243..9289
                     /gene="galT"
                     /locus_tag="OO150_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5515457.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9293..10441
                     /gene="galK"
                     /locus_tag="OO150_001248"
     CDS             9293..10441
                     /gene="galK"
                     /locus_tag="OO150_001248"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5515458.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10435..11475
                     /gene="galM"
                     /locus_tag="OO150_001249"
     CDS             10435..11475
                     /gene="galM"
                     /locus_tag="OO150_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5515459.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11677..12429
                     /gene="gpmA"
                     /locus_tag="OO150_001250"
     CDS             11677..12429
                     /gene="gpmA"
                     /locus_tag="OO150_001250"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5515460.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12587..13639)
                     /gene="aroG"
                     /locus_tag="OO150_001251"
     CDS             complement(12587..13639)
                     /gene="aroG"
                     /locus_tag="OO150_001251"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5515461.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13955..14335
                     /locus_tag="OO150_001252"
     CDS             13955..14335
                     /locus_tag="OO150_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5515462.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14449..15390
                     /gene="zitB"
                     /locus_tag="OO150_001253"
     CDS             14449..15390
                     /gene="zitB"
                     /locus_tag="OO150_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5515463.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHAHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15387..16106)
                     /gene="pnuC"
                     /locus_tag="OO150_001254"
     CDS             complement(15387..16106)
                     /gene="pnuC"
                     /locus_tag="OO150_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5515464.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(16144..17187)
                     /gene="nadA"
                     /locus_tag="OO150_001255"
     CDS             complement(16144..17187)
                     /gene="nadA"
                     /locus_tag="OO150_001255"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5515465.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17436..17511)
                     /locus_tag="OO150_001256"
     tRNA            complement(17436..17511)
                     /locus_tag="OO150_001256"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17476..17478),aa:Lys,seq:ttt)
     gene            complement(17658..17733)
                     /locus_tag="OO150_001257"
     tRNA            complement(17658..17733)
                     /locus_tag="OO150_001257"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17698..17700),aa:Lys,seq:ttt)
     gene            complement(17737..17812)
                     /locus_tag="OO150_001258"
     tRNA            complement(17737..17812)
                     /locus_tag="OO150_001258"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17777..17779),aa:Val,seq:tac)
     gene            complement(17864..17939)
                     /locus_tag="OO150_001259"
     tRNA            complement(17864..17939)
                     /locus_tag="OO150_001259"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17904..17906),aa:Lys,seq:ttt)
     gene            complement(17942..18017)
                     /locus_tag="OO150_001260"
     tRNA            complement(17942..18017)
                     /locus_tag="OO150_001260"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17982..17984),aa:Val,seq:tac)
     gene            complement(18053..18128)
                     /locus_tag="OO150_001261"
     tRNA            complement(18053..18128)
                     /locus_tag="OO150_001261"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18093..18095),aa:Lys,seq:ttt)
     gene            complement(18293..19084)
                     /gene="cpoB"
                     /locus_tag="OO150_001262"
     CDS             complement(18293..19084)
                     /gene="cpoB"
                     /locus_tag="OO150_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5515466.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(19094..19615)
                     /gene="pal"
                     /locus_tag="OO150_001263"
     CDS             complement(19094..19615)
                     /gene="pal"
                     /locus_tag="OO150_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5515467.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19650..20942)
                     /gene="tolB"
                     /locus_tag="OO150_001264"
     CDS             complement(19650..20942)
                     /gene="tolB"
                     /locus_tag="OO150_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5515468.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLVFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(21075..22256)
                     /gene="tolA"
                     /locus_tag="OO150_001265"
     CDS             complement(21075..22256)
                     /gene="tolA"
                     /locus_tag="OO150_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5515469.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAADKKAAEKAAAEKAAADKKAAAEKAAAEADDIFGELSSG
                     KNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKT
                     CTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
                     P"
     gene            complement(22321..22749)
                     /gene="tolR"
                     /locus_tag="OO150_001266"
     CDS             complement(22321..22749)
                     /gene="tolR"
                     /locus_tag="OO150_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5515470.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22753..23445)
                     /gene="tolQ"
                     /locus_tag="OO150_001267"
     CDS             complement(22753..23445)
                     /gene="tolQ"
                     /locus_tag="OO150_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5515471.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23442..23846)
                     /gene="ybgC"
                     /locus_tag="OO150_001268"
     CDS             complement(23442..23846)
                     /gene="ybgC"
                     /locus_tag="OO150_001268"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5515472.1"
                     /translation="MNTTLFRWPARVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23996..24289)
                     /gene="ybgE"
                     /locus_tag="OO150_001269"
     CDS             complement(23996..24289)
                     /gene="ybgE"
                     /locus_tag="OO150_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5515473.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            complement(24289..24402)
                     /gene="cydX"
                     /locus_tag="OO150_001270"
     CDS             complement(24289..24402)
                     /gene="cydX"
                     /locus_tag="OO150_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5515474.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24417..25556)
                     /gene="cydB"
                     /locus_tag="OO150_001271"
     CDS             complement(24417..25556)
                     /gene="cydB"
                     /locus_tag="OO150_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5515475.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25572..27140)
                     /gene="cydA"
                     /locus_tag="OO150_001272"
     CDS             complement(25572..27140)
                     /gene="cydA"
                     /locus_tag="OO150_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5515476.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27988..>28862)
                     /locus_tag="OO150_001273"
     CDS             complement(27988..>28862)
                     /locus_tag="OO150_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5515477.1"
                     /translation="GVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTPFNTDSVL
                     ADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVFSEGLREF
                     EVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSCRLSLLSY
                     TGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPPVGCLISA
                     LKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQGSNLSGP
                     FLPGQSRTFSYRLA"
BASE COUNT         7300 a         7584 c         7042 g         6936 t
ORIGIN      
        1 gacattttta aaacagctat agcttctggt cttgccgaag aagatccagc ttctgcaacc
       61 aggccattca gaactgaagt gaagcgcagt cgactgttaa ttgacgagta tctgttaatc
      121 aggaaaatag cagacgtcca gaacgaatgg ttcggccttt gcatggatct tgcactggta
      181 acaggacagc gcgaaggaga tctggcggct atgagatggg aggatataag agatggcaga
      241 ctatacgtgg aacaacaaaa aacaggggca aaaattcgca tttctctccc caccacgatt
      301 tccagattga atttaacact taccgatgta ctggataact tgaaaaaaat aaacggtaaa
      361 aacgaaaagt tactcggagg gaagacagcc aggacaatcg cagcacagtt caggattgcc
      421 cgagacacat ccggattgaa atgggaaggt gacccaccgc catttcatga aatccgaagt
      481 ttatccggtc gcctgcactc agcagaaaaa ggcagtgatt tcactcaggc gctccttgga
      541 catcgttcat ccagcatgac ggataaatac cgtgacggac gagggaggga atggaaagac
      601 atttagaatt aatttcgaat gatttcgaaa caccacttgt aattaattga ttataaagga
      661 ttaattccta agaggcattt gcttcatgaa tgctcctttg catttagcaa ctgactgtaa
      721 cagctaaaat tagtcgcttt tggcggtgaa tgctgaatta aaacgcagaa ttttgtccga
      781 tagtggtatc ggtgtaccat tcgcgcagac gaaaattctt aaccttaatc tggaatacgc
      841 catgaccaca cgcgtgattg ctctcgactt agacggcacc ttattgaccc cgaaaaagac
      901 cctgcttcct tcatcgatag aagccctggc ccgcgctcgc gaagcaggct atcaattaat
      961 catcgtcaca ggtcgccatc acgtcgctat tcatcctttt tatcaggcgc tggcgctgga
     1021 tacacctgct atttgctgta atggcaccta tttgtatgat tatcatgcaa aaaccgtgct
     1081 ggaagcggac ccaatgcccg ttaataaagc cctgcaactc attgagatgc tgaatgaaca
     1141 ccacattcac ggtctgatgt atgtggatga tgcaatggtc tatgagcacc cgaccgggca
     1201 tgtcattcgc acgtctaact gggcgcaaac cctgccgccg gaacagcgtc cgactttcac
     1261 acaagtcgct tctctggctg aaacggcgca acaagttaac gccgtatgga agttcgccct
     1321 gacgcacgat gacatgccgc aattgcagca ttttggtaag catgtcgaac atgaactggg
     1381 actggagtgt gaatggtcct ggcacgatca ggttgatatt gcacgcggcg gcaacagcaa
     1441 aggtaaacgt ttgacgaaat gggttgaggc gcaaggctgg tcgatggaaa acgtcgtggc
     1501 gttcggcgat aactttaatg atatcagtat gctggaggcc gctggtacag gcgtggcgat
     1561 gggcaacgcc gatgacgcgg taaaagcgcg cgccaacatt gtgattggtg ataacaccac
     1621 cgacagcatt gcccagttca tttatagcca cctgatttaa tcaggcggtt atcgacacac
     1681 ttttaatttg cgcgtacagc cacaggcctg gtttgatcgc cagttcatcc ctggcccacg
     1741 ggctgatacg cgcccacagc gttttaccgc cgacttccag ctccacttcc acctgaccgt
     1801 tgtcgtcata actattaaca acttttgccc gcaatacgtt acgaatgctg gtttgctgcg
     1861 gcggttgtaa aaccagagaa acatcggaag cctgaatgcg gatacgtagc gcagcttgca
     1921 gcggttcgtc cagcttattg acccacaaat gctgatcgcc cagcgccagc gcggtcatcg
     1981 cgtaatgcgg atgatgctca agcaccgtca ctttcagaat gctactttgt tgctctttcg
     2041 gcagccacgg attcatcacg ctactacccc agacttcttc cagtgcgcca aaggctttca
     2101 cctgaccgtt ttccagtacc atcactctgt ctgccagatg gaggatctca tccagcgaat
     2161 ggctgacata caacatcggg atgttgattt cccgcgtcag acgttgcaga taaggcaaca
     2221 gttcgcgttt acgcggaata tccagtgacg ccagcggttc atccagcaac agcaattctg
     2281 gtgccgtcag caaagcccgc ccaatcgcta cgcgctgttt ttcgcctccg gacaggctgc
     2341 ctggtaaacg gtcaagcaac ggttcaatgc ctaaaagcgc caccagctta tcgaactgat
     2401 cgaccatgct tttcgccatg ccgtagcgca gattgccacg cactttgtaa tgcggaaaca
     2461 gccgcgcatc ctgaaaaaca tagccaacgc gacgcttttc tggcgtcagg cagatacctt
     2521 tttcggcatc atttagtacc cgaccattga ggacaatcct ccctttttgc gggcgcgtca
     2581 gtccactgat ggcgttaatc agcgaagttt ttccggcacc ggagacgcca aagatagcag
     2641 tgatgccatt ggcgggcagc gtttcattaa tagtcaggca atggttgccc aacgtctggg
     2701 aaaaattcag ttccagcatg attagcgccc cgcccgttca cggctgattc tggccagcca
     2761 ttctgaaatc aacagggaga tcatcgccag cgcaatagaa ataatgcaca gtctcgccgc
     2821 gccactttcc ccgccggggg tctggatcag ggtatacatg gcagagggaa tggttcgcgt
     2881 ttcgccagga atgttcgaca caaaggtgat ggttgcacca aactcaccga gagaacgagc
     2941 aaaagccagt accgtaccaa caataattcc cggtaaggtc agcggtaacg tgatagtaaa
     3001 gaaaacgcgc cagcgcccgg cacctagtgt tcttgcggcc tgttcgagtt tgacatcaac
     3061 cccttccagc gccagacgaa ttgcccgcac catcagcgga aacgacatga cggcggcagc
     3121 gagaaccgcg ccgcgccagc taaaggcgaa ggtaatacca aaccagtcat acagacgttc
     3181 accgataaat ccgcgccgtc ccatcgaaac taataataag taaccgacga ccacgggcgg
     3241 taacaccagc ggtagatgca gtacgctgtc gagcagagct ttgcccggaa acgtgcaacg
     3301 caccagtaac caggcaaaaa agatcccaaa cggcaggcta aacagcacag ccagggaaga
     3361 aactttcagg cttaataaaa ctgcctgcca ttctggatcg gtcagtatca ttacttggtt
     3421 gtaaatccgt aacgtttaaa gatttccgct gcctgcggtc ccttcagata atcataaaat
     3481 gctttcactg tcgcattgtt atgcccttcc acaacagcaa ccggatattc cactttttta
     3541 tgtgaatctt ccgggaaggt ggcaaccact tttacccctt tgctgacaac tgcgtcagaa
     3601 ccgtaaacaa tgcccaacgg cgcttcattg cgttcgacca acgccagcgc gccacgaaca
     3661 tcttccgccg gagccaattt cggggagagc gtatcccatg cgcccagttt ttgcagtgct
     3721 tcttttgcat aaatgccagc agggacatgt tccggatcgc caaccgcgag gcgaccgcca
     3781 ttcagcagcg aagtccagtt agttgtgctg tcgatgatga aatctttctg ctcgctggct
     3841 ttcggtgcta caacgaccag gctattgccg agcagtgtct ggcgcgaagc cgtatcgatc
     3901 gcttttttat caaccgcata atccatccat ttctgatcgg cagagataaa cagatccgca
     3961 ggcgctcccg cttcaatctg acgggccaga gtagatgacg aagcgaaaga agaaaccaca
     4021 tccacgcctt tttctttttt atactgcgta gcaatgtcct gcattgcgtt agtcagtgat
     4081 gcggcggcga acaccgtgat tttcccttca tctgccagtg tattgccagc aacagcgaaa
     4141 gagagtgccg ccccggcaaa caagttcaac catttacgag ccattggtaa ccccttaatg
     4201 taacgttata taggtggcaa tataacggct aactcagaat tttcccagca gttattgcta
     4261 acctttgtga ggtagataag aaaaaatatc ggctggaaaa gcaggaagtt gagaaaaaga
     4321 aatgcccgac taagtgggca ttcagggaat caatgatttt gtccgggctg gtctttttta
     4381 ccaacaccag aaaagatgtt gaatacttca ccaagaccgt aaatcagacc caggatgatg
     4441 gccatcacga caggtaccat gattacggcg aataccagac tttttaataa ctctaacatg
     4501 gtcaactcca gatatagtca tgagactatt ctaaccgcta agcacagaaa agcactcccc
     4561 ttttgtgcgg tcagctttgc gtggcgttcg ttttccgtca caataagact tttgccagga
     4621 cattgttatg caggccgaaa tccttctcac ccttaagctc caacaaaaat tattcgccga
     4681 cccgcgccgg atttcgctac taaaacacat tgcgctttcc ggttccatta gccagggagc
     4741 gaaagatgcc ggtatcagct ataaaagcgc ctgggatgcc attaacgaga tgaatcagtt
     4801 aagcgagcat attctggttg agcgcgcaac aggcggtaaa ggtggcggcg gcgcagtact
     4861 gacccgctat ggtcagcgac tgattcagct ctatgactta ctggcgcaaa tccagcaaaa
     4921 agcctttgat gtgttaagtg acgatgacgc cctgccgctg aacagcctgc tggccgcgat
     4981 ctcacgtttt tcactgcaaa ccagcgcccg taaccagtgg ttcggtacca tcaccgcccg
     5041 cgatcatgat gacgttcaac agcatgttga tgtcttactg gctgacggaa aaacacgcct
     5101 gaaagtcgca attaccgcac aaagcggcgc acgtctgggg ctggatgaag gcaaagaagt
     5161 gttgatattg ctaaaagcgc cgtgggtagg cattactcag gacgaagcgg tcgcacaaaa
     5221 cgccaacaac caattaccgg gtattattag tcatattgag cgcggcgcag agcagtgcga
     5281 agtattaatg gcgctacccg acgggcaaac actgtgcgcc acagtgccgg taaatgaagc
     5341 gacttctctt cagcaagggc agaatgtcac ggcctacttt aatgccgaca gcgtgattat
     5401 cgccacgctg tgctaagcgt gttgacaatt tgttatgaaa cacgtatccc tgtcagtaat
     5461 cgctgcacaa aatgggatat aaaatgtcat cgttgcaaat tttgcaaggc acgtttcgtc
     5521 ttagcgacac aaaaacgctg caattgcctc agctaacgtt aaacgcgggt gatagttggg
     5581 cgtttgtcgg ttcgaatgga agcgggaaat cggccctggc ccgcgcgctg gcgggggaac
     5641 ttccgctttt gaaaggtgaa cggcaaagcc agttttccca catcactcgt ctctccttcg
     5701 agcaattgca aaaactcgtc agcgacgaat ggcagcggaa taacaccgat atgctcggcc
     5761 ctggcgaaga tgacaccgga cgcactacgg ctgagatcat tcaggatgaa gtaaaggatg
     5821 caccgcgttg catgcaactg gcgcagcagt tcggtattac cgccctcctc gaccgacgct
     5881 ttaaatacct ttccactggc gagacgcgaa aaatcctgct gtgtcaggcg ctgatgtcgg
     5941 agcctgactt gttgattctt gatgagccgt tcgatggcct ggatgttgcc tcacgtcagc
     6001 agctggctga gcgactcgcc tcgttacatc agtccggtat tactctggta ctggtgctca
     6061 atcgcttcga tgagatcccg gagtttgtcc agtttgctgg cgtgctggcg gattgcacgt
     6121 tagcggaaac tggcgctaaa gaggaactgc tccaacaagc actcgtcgcg caactggcac
     6181 atagtgaaca gcttgaaggt gtgcaactgc cggagccgga tgaaccttca gcacgtcacg
     6241 ccttacccgc caacgaaccg cgcattgtgc tgaacaatgg cgtggtttct tataacgatc
     6301 gccccattct taataacctt agctggcagg tgaatccagg tgaacactgg caaattgtcg
     6361 ggccaaatgg tgcaggaaaa tcgacgttat taagcctggt tactggcgat catccgcaag
     6421 gttacagcaa cgatttgacg cttttcggac gacgtcgcgg cagcggcgaa accatctggg
     6481 atatcaaaaa gcatatcggt tacgtcagca gtagtttgca tctggattac cgggtcagca
     6541 ctaccgtgcg taatgtgatt ctttctggct attttgattc gattggcatt tatcaggccg
     6601 tttcggatcg ccagcaaaaa ctggtgcagc agtggctgga tattctcggc attgataaac
     6661 gtacggctga cgctccgttc catagtcttt cctggggaca gcagcatctg gcgctgatcg
     6721 tccgcgcact ggtgaaacat ccgacgttgc ttattctcga tgaaccacta caggggcttg
     6781 atccgctcaa tcgccagctt atccgccgtt ttgttgatgt gctgattagc gaaggtgaaa
     6841 cgcaattgtt gtttgtttcg caccacgctg aagatgcgcc tgcctgtatt acccatcgcc
     6901 ttgagttcgt gccggacggt ggactctatc gctatgtgct gacaaaaata tattgagtcg
     6961 gtagtgctga ccttgccgga ggcggcctaa gcgccctctc cggccaacgg ttcgacgcat
     7021 gcaggcatga aaccgcttct tttttcagat aaaaagcgca atcattcata aaccctctgt
     7081 tttataatca cttaatcgcg cataaaaaac ggctaaattc ttgtgtaaac gattccacta
     7141 atttattcca tgtcacactt ttcgcatctt tgttatgcta tggttatttc ataccataag
     7201 cctaatggag cgaattatga gagttctgct taccggtggt agcggttaca ttggaagtca
     7261 tacctgcgtg caattactgc aaaacggtca tgatgtcatc attcttgata acctctgtaa
     7321 cagtaagcgc agcgtactgc ctgttatcga gcgtttaggc ggcaaacatc caacgtttgt
     7381 tgaaggcgat attcgtaacg aagcgttgat gaccgagatc ctgcacgatc acgctatcga
     7441 caccgtgatc cacttcgccg ggctgaaagc cgttggcgaa tcggtacaaa aaccgctgga
     7501 atattacgac aacaatgtca acggtactct gcgcctgatt agcgccatgc gcgccgctaa
     7561 cgtcaaaaac tttattttta gctcctccgc caccgtttat ggcgatcagc ccaaaattcc
     7621 atacgttgaa agcttcccga ccggcacacc gcaaagccct tacggcaaaa gcaagctgat
     7681 ggtggaacag atcctcaccg atctgcaaaa agcccagccg gactggagca ttgccctgct
     7741 gcgctacttc aacccggttg gcgcgcatcc gtcgggcgat atgggcgaag atccgcaagg
     7801 cattccgaat aacctgatgc catacatcgc ccaggttgct gtaggccgtc gcgactcgct
     7861 ggcgattttt ggtaacgatt atccgaccga agatggtact ggcgtacgcg attacatcca
     7921 cgtaatggat ctggcggacg gtcacgtcgt ggcgatggaa aaactggcga acaagccagg
     7981 cgtacacatc tacaacctcg gcgctggcat aggcagcagc gtgctggacg tggttaatgc
     8041 cttcagcaaa gcctgcggca aaccggttaa ctatcatttt gcaccgcgtc gcgagggcga
     8101 ccttccggcc tactgggcgg acgccagcaa agccgaccgt gaactgaact ggcgcgtaac
     8161 gcgcacactc gatgaaatgg cgcaggacac ctggcactgg cagtcacgcc atccacaggg
     8221 atatcccgat taaggaacga ccatgacgca atttaatccc gttgatcatc cacatcgccg
     8281 ctacaacccg ctcaccgggc aatggattct ggtttcaccg caccgcgcta agcgcccctg
     8341 gcagggggcg caggaaacgc cagccaaaca ggtgttacct gcgcacgatc cagattgctt
     8401 cctctgcgca ggtaatgtgc gggtgacagg cgataaaaac cacgattaca ccgggactta
     8461 cgttttcact aatgactttg cggccttgat gtctgacacg ccagatgcgc cagaaagcaa
     8521 cgatcctttg atgcgttgcc agagcgcgcg cggtaccagc cgggtgatct gcttttcacc
     8581 ggatcacagt aaaacgttgc cagaactgag cgttgcggca ttgacggaaa tcgtcaaaac
     8641 ctggcaggag caaaccgcag agctgggaaa aacgtacccg tgggtgcagg tctttgaaaa
     8701 caaaggtgcg gcgatgggct gctctaaccc gcatccgcac ggacagattt gggcaaatag
     8761 cttcctgcct aacgaagctg agcgcgaaga ccgcctgcaa aaagaatatt ttgccgaaca
     8821 gaaatcacca atgctggtgg attatgttca gcgcgagctg gcagacggta gccgtaccgt
     8881 tgtcgaaacc gaacactggt tagccgttgt gccttactgg gctgcctggc cgttcgaaac
     8941 gctactgctg cccaaagccc acgttttgcg gatcaccgat ttgaccgacg cccagcgcag
     9001 cgatttggcg ctggcgttga aaaagctgac cagtcgttat gacaacctct tccagtgctc
     9061 cttcccctac tctatgggct ggcacggcgc accatttaat ggcgaagaga atcaacactg
     9121 gcagctgcac gcgcactttt atccgcctct gctgcgctcc gccaccgtac gtaaatttat
     9181 ggttggttat gaaatgctgg cagagaccca gcgagacctg accgcagaac aggcagcaga
     9241 gcgtttgcgc gcagtcagcg atatccattt tcgcgaatcc ggagtgtaag aaatgagtct
     9301 gaaagaaaaa acacaatctc tgtttgccaa cgcatttggc taccctgcca ctcacaccat
     9361 tcaggcgcct ggccgcgtga atttgattgg tgaacacacg gactacaacg acggtttcgt
     9421 tctgccctgc gcgattgatt atcaaaccgt gatcagctgt gcaccacgcg atgaccgtaa
     9481 agttcgcgtg atggcagccg attatgaaaa tcagctcgac gagttttccc tcaatgcgcc
     9541 cattgtcgcg catgaaaact atcaatgggc gaactacgtt cgtggcgtgg tgaaacatct
     9601 gcaactgcgt aacaacagct tcggcggtgt ggacatggtg atcagcggca atgtgccgca
     9661 gggtgccggg ttaagttctt ccgcttcact ggaagtcgcg gtcggaaccg tattgcagca
     9721 gctttatcat ctgccgctgg acggcgcaca aatcgcgctt aacggtcagg aagcagaaaa
     9781 ccagtttgtt ggctgtaact gcgggatcat ggatcagcta atttccgcac tcggcaagaa
     9841 agatcatgcc ttgctgattg actgtcgttc actggggacc aaagcagttt ccatgccgaa
     9901 aggtgtggct gtcgtcatca tcaacagtaa cttcaaacgt accctggttg gcagcgaata
     9961 caacacccgt cgtgaacagt gcgaaaccgg tgcgcgtttc ttccagcagc cagccctgcg
    10021 cgatgtcacc attgaagagt tcaacgctgt tgcacatgag ctggacccaa tcgtggcgaa
    10081 acgcgtgcgg catatcctga ctgaaaacgc ccgcaccgtt gaagctgcca gcgcgctgga
    10141 gcagggcgac ctgaaacgta tgggcgagtt gatggcggag tctcatgcct ctatgcgcga
    10201 tgatttcgaa atcaccgtgc cgcaaattga cactctggta gaaatcgtca aagctgtgat
    10261 tggcgacaaa ggtggcgtac gcatgaccgg cggcggattt ggcggctgta tcgtcgcgtt
    10321 gatcccggaa gagctggtgc ctgccgtaca gcaagctgtc gctgaacaat atgaagcaaa
    10381 aacaggtatt aaagagactt tttacgtttg taaaccatca caaggagcag gacagtgctg
    10441 aacgaaactc ccgcactggc acccgatggt cagccgtacc gactgttaac tttgcgtaac
    10501 aacgcaggga tggtagtcac gctgatggac tggggtgcga ctttactttc agcccgtatt
    10561 ccgctttccg atggcagcgt ccgcgaggcg ctgctcggct gtgccagccc ggaatgctat
    10621 caggatcagg ccgcgtttct gggggcctct attggtcgtt atgccaaccg catcgccaat
    10681 agccgttata cctttgacgg tgaaaccgtg accctttcgc caagtcaggg cgttaaccag
    10741 ctgcacggcg gcccggaagg gttcgacaaa cgtcgctggc agattgtgaa ccagaacgat
    10801 cgtcaggtgc tgtttgccct gagttcagat gatggcgatc agggcttccc gggtaatctc
    10861 ggcgcaacgg tgcaatatcg tctgaccgac gataaccgta tctccattac ttatcgcgcc
    10921 acagttgata aaccttgccc ggtgaatatg actaatcacg tctatttcaa tcttgacggc
    10981 gagcagtctg acgtgcgcaa tcacaagttg cagattctgg cggacgaata tctgccggtt
    11041 gatgaaggcg gcattccgca cgacggcctg aaatctgtcg ccggaacgtc ttttgatttc
    11101 cgcagcgcca aaatcatcgc cagtgagttt cttgccgacg acgatcagcg caaagtgaaa
    11161 ggttacgatc acgcattctt gttacaggcc aaaggcgatg gcaagaaagt ggcggcgcat
    11221 gtctggtcag cagatgaaaa attgcagctg aaggtctaca ccaccgctcc ggctctgcaa
    11281 ttctactccg gcaacttcct cggcggcaca ccgtcgcggg gaacagaacc ttacgccgac
    11341 tggcaagggc tggcgttgga aagcgagttt ctgccggaca gcccgaacca ccctgaatgg
    11401 ccgcaaccgg actgcttcct gcgtcctggc gaagagtatt ccagcctgac ggaatatcag
    11461 tttattgctg agtaatgtta agccctcgtc atgagggctt tatctcatat tgctcaaatc
    11521 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    11581 attttcgcta tggttatgcg taagcattgc tgttgcttcg tcgcggcaat ataatgagaa
    11641 ttattatcat taaaagatga tttgaggagt aagtatatgg ctgtaactaa gctggttctg
    11701 gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg
    11761 gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa
    11821 ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg
    11881 aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac
    11941 gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaactgctga aaagtatggc
    12001 gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga ctccgccgga actgactaaa
    12061 gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagaactg
    12121 ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact
    12181 attctaccgc gtatgaagag cggtgagcgc gtaatcatcg ccgcacacgg taactccctg
    12241 cgtgcgctgg tgaaatatct tgataacatg agcgaagaag agattcttga gctgaatatc
    12301 ccgactggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat
    12361 tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgcaaa ccagggtaaa
    12421 gcgaagtaaa cgtcattcgt ttaaaatgag aaagccgact gcaagtgagt cggctttttt
    12481 gttgctaaca atggagcaca ttgcctgatg cgacgcttcg cgacttatca ggcctgtggt
    12541 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    12601 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccgatgcagg
    12661 catcggtgat gctcttaccg taggccagcg gctccccgct ctcgaggctc tgattgcctt
    12721 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    12781 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    12841 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12901 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcgc
    12961 cgttaccgct ggtattcaca atcgccgaat gcccccattt cgttacggac aggaagcagt
    13021 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tcggtgccat
    13081 ttttgaagcc gactggacaa gaaagccctg atgccagttc gcggtgcacc tgcgattcgg
    13141 tggtacgtgc gccaattgcg ccccagctca tcaggtcagc gagatattgt ggggtgatca
    13201 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    13261 gggctatacg aagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    13321 gccctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    13381 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    13441 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    13501 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagtagcgg
    13561 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    13621 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    13681 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacatta tgaacggatt
    13741 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    13801 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    13861 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13921 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13981 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    14041 gcaaactaat aacggccagg caaacgccgc aactgatgcg ggccaggtag cccctgacgc
    14101 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    14161 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaga
    14221 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaac
    14281 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    14341 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    14401 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    14461 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    14521 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    14581 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    14641 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    14701 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    14761 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    14821 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    14881 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14941 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    15001 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    15061 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    15121 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    15181 tggatggtag gtgagaagcc ggtgatgacg ctgcatgtgc aggtgatccc gccacacgct
    15241 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    15301 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    15361 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcatgagcc tctttcacgt
    15421 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    15481 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    15541 acccacaaca gccagttttc cacatactta cgcgtcatca gaagcattgc cacgatagat
    15601 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    15661 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    15721 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    15781 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    15841 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15901 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15961 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    16021 cctaccgctt cgatccatga gagatcataa ccgcctgctc ctattggtat atgtaccagg
    16081 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    16141 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    16201 ctctcgcagc ctttcatcaa catgaacctc gtgattgctt ccttcctgtt ctaatgcctc
    16261 tgcgatggcc tgaaggccat tcatggccat ccacggacaa tgcgcgcagc tgcggcaggt
    16321 tgcaccctca cctgcggttg gtgcttccag taactcttta tctggcaccg cctgctgcat
    16381 tttgtagaaa ataccccgat cggttgccac aataagcctc tgatgtggca atgttttcgc
    16441 agcagcgatc agttgactgg tggaaccgac cgcatccgcc atatcgacaa tagcttgtgg
    16501 tgattctgga tgcaccagta tggcagcatc cgggtattct tcttgcaagc gggttaacgc
    16561 ctgagtctta aattcatcat gcacaataca ggcaccctgc cagcatagaa tgtctccacc
    16621 cgtctgtttt tgcacgtaac gccccagatg tttgtcgggt gcccagatga ttttttcacc
    16681 caaactatca agatgatcaa taagttcgac ggcaatgctt gaagttacca cccaatctgc
    16741 gcgcgctttt accgcagcag aagtgttggc gtagacgacg acagtacgat cgggatgggc
    16801 atcgcaaaat gcgttaaatt cttcaacagg gcagccgaga tccagtgaac attcagcctg
    16861 aagtgtcggc atcagaattg ttttttccgg actgagaatt ttggcggttt ctcccataaa
    16921 tctcacccca gcgactaaca aagtagaagc gggatgcttt gcaccgaagc gcgccatttc
    16981 cagagaatca gaaatacagc caccggtttc ttctgccagt tgttgaattt cgggatcggt
    17041 atagtagtgg gcaaccatca ccgcattacg ttcttttagc agacgtttta tcttctcgcg
    17101 gtaatacgct ttttcatcaa tgcttaacgg cgtcggcttc ggggggaaag gataaatcgc
    17161 cgtgtctgga tcaaacatta cgctcatctt accatctcgt tttacaggct taacgttaaa
    17221 accgacatta gcgtaatatt cgctgttatc ggtgtaattt gttttatatg ctaaacaaga
    17281 tagtcgaatg gtggggaaaa gtcactcgaa ttacgcctaa ttccaccgac gtagagttgc
    17341 ttgcacagta agctctgacg atgtcatcag gtttacaaag tgaaattgag gatgcctgaa
    17401 tagcaaaaaa gcgcctttag ggcgcttttt tacattggtg ggtcgtgcag gattcgaacc
    17461 tgcgaccaat tgattaaaag tcaactgctc taccaactga gctaacgacc catacgggtg
    17521 ttacctgaaa gatttctact cggggccaat ctttaagatt ggtgtgtcgt gcaggatgac
    17581 tcgcttcgct tgccctacgg gccgttgctg gcgcaacgtt atccttcacg cttaacatct
    17641 gagtttgatg ttaaaattgg tgggtcgtgc aggattcgaa cctgcgacca attgattaaa
    17701 agtcaactgc tctaccaact gagctaacga cccgagtggt gggtgatgac gggatcgaac
    17761 cgccgacccc ctccttgtaa gggaggtgct ctcccagctg agctaatcac ccgatactgc
    17821 gctggatact acaaactact tccaccgaac ccatcgtaat tagtggtggg tcgtgcagga
    17881 ttcgaacctg cgaccaattg attaaaagtc aactgctcta ccgactgagc taacgacccg
    17941 gtggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc
    18001 agctgagcca atcacccaat actacgctgg atactacaac taactatgtt agtggtgggt
    18061 cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct
    18121 aacgacccac tttttcgttg ctttcggttt gtttgatatc ccgtggcaac ggcggcatat
    18181 attactgatt tcagacttga gcgcaacaaa aatttcgatg cagatcactc aactgcttat
    18241 gattcgcacg acacgaccag aaataatgca acttctggtc gtgtgttatg cattacatcg
    18301 cgttcagacg tttttgtgcc tgtttagcgc catcagtacc agggtattta ctgataacct
    18361 gctggtatac ggctttcgct tttgcggtgt cacctttgtc ctgcatgatg acgccgactt
    18421 taaacatcgc atcagcagcc tttggtgact tcggatagtt tttcactacc gaagcaaaat
    18481 agtacgccgc atcatctttt ttacccttgt tgtagtttaa ctgacccagc caataattgg
    18541 cgtttggcag gtaagttgaa tcagggtaat ttttgatgaa attctgaaat gccaccattg
    18601 cgtcatcctg gcgggattta tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg
    18661 catcgccgct tttcaccggc gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg
    18721 ttgatgccgc cgcaccgctt tgatcgctgc tggttgattg cgccgctgca ccaccgctgc
    18781 tgaggctgtc gatctgcaac aggatctgct tttgccgctc cacgacctga ttcagttgat
    18841 actgattttc ctgaatctga ccacgcaggg aatcaatatc ggattgatta tcagaaagtt
    18901 gttgctggag ttgggttaaa agctggctgt gagcattaga aatacgctca agttgagtga
    18961 cgcggtcttc gaccgagcct gagccgacac tactgattgg tgcctgagca aaagcggccc
    19021 agggggccgc tataccaacc agtaacgaca gactcaatag ttgatgtctg aagttactgc
    19081 tcatgcaatt ctcttagtaa accagtaccg cacgacggtt tttggagtat gccgcttcgt
    19141 catgacccag tactgcaggt ttttctttac cgtaagaaac gatggagatc tggtctgcag
    19201 aaacgccttt accctgcagg tacatcttaa cggcgttcgc acgacgttca cccagggaga
    19261 tgttgtattc cggagtacca cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg
    19321 ggttgctacg caggaagttt gcatgtgcat ccagcatttg agcgaagtca gaacggatat
    19381 cgtacttgtc cagatcgaag taaacgatgt tgttctgctg cagctgttgc atttgcagac
    19441 gagcctgctc ttcggaagac atgttgccgt tgccgccgtt cgcatccata ccagtgccgg
    19501 cacccagcat gccttcgctg ccgtcattgc tggcgttctt gttggaagaa catgccgcaa
    19561 ttgccataac aggcagagca atcatcagcc ctttcagcac tttgttcagt tgcatttcaa
    19621 tgattccttt actattcaat taattattat cacagatacg gcgaccaggc agggaatttg
    19681 acctgtccat cagttgccgg aagacgcgct ttgaaacgcc catctgtaga aaccaaattc
    19741 agcacggatc ccatcccctg agaagagctg tagattacca tagtgccatt aggtgccaga
    19801 cttggcgttt catccaggaa cgtggacgac agaacttgta cgcctcccgt tgccagatct
    19861 tgtttggcaa tgtgctgctg cccaccattg gagctgacca ttaccataaa tttaccgtcg
    19921 ctgctgacat ccgcatcctg gttctgcgaa ccttcccagg taatacgttg tggcgcaccg
    19981 ccgttgatat tcactttata aacctgcgga cgaccggcct ggtcagaagt aaataccagg
    20041 ttctggctat ccgggaacca ggtcggttcg gtattgttac tgcgaccatc agtcacctgg
    20101 cggatctgac cagaagccaa atccattacg tacaggttca gactaccggt tttcgacaag
    20161 gcgaatgcca gtttgctgcc gtctggcgag aatgcaggtg caccgttgtg acgcgggaat
    20221 gaagccacct gacgtacagc gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg
    20281 ctttcgaagg tcacataagc cagtttagaa ccgtctggtg accacgccgg agacatcagc
    20341 ggctgcggtg aacggtgaac gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt
    20401 tcatacggga actgaccgcc gttggtctga acaacgtagg caatacgggt acggaacgca
    20461 cctttaatgc cggtcagttt ttcaaacact tcatcactgg cggtatgacc agcataacgc
    20521 agccactgct tgttcacttt gtacgagttc tgagcaagta cagtacccgg tgcgccgcca
    20581 gtgtcaacaa gttgataagc aacattgtaa gaaccatccg gattcggagt gacctgaccg
    20641 acaactacag cgtcaatgcc cagtgcggac catgcagatg gttgtacttc ctgcgcacta
    20701 cccggctgct gtggcagacg agcgcgatct aacggattaa atttaccgct gttacgcaag
    20761 tcagcagcaa cgatgccgcc aatatcttca ggtgccgcac caggccccgc ccactggaaa
    20821 ggaacaacac caataggacg accggaatct acaccgctgt cgatcacaat gcggacttca
    20881 gcatgcagaa ctgatgccca cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc
    20941 atcatatctc ccttatctgg accgatggcc cacgataatt tagcagaatg ttaacaaact
    21001 aaaatacaca aaactaccag aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac
    21061 agtcaacatc gcgattacgg tttgaagtcc aatggcgcgt ttttgaacac ttcatatact
    21121 gcctggcttg gtggtttcgg gatcttcgca agtttagctg ctgccaacgc agcctgacaa
    21181 agtgcgggat cgccaccttc aggtttgata tccagtaaca taccatcggg tgccagtttt
    21241 atgcgcagcg tacaggtttt gcctgcatag gacgatgcgt catagaactt actttcgata
    21301 gcagatttaa tctgcccggc atagttattg atatcggccc ctgatgcgcc attgttttta
    21361 gtattaccac tcccggcagg cgaagcattg ttccctttcg cccctccccc cgttttcggt
    21421 gcattcttac cagagcttag ctcaccgaaa atatcatctg cctctgcggc ggctttttct
    21481 gccgctgctt tcttatcagc tgctgccttt tcagcagccg ctttttctgc tgcttttttg
    21541 tcggctgcag ctttcttatc agctgcagcc ttttcagcag ccgctttctt ctcagcttct
    21601 gctttggctt tttcagcagc ttcagttgcc gctttctttc ttgcttcagc tgcagctgct
    21661 tctgccgctt ccgctttctt cttcagtgcc gcagcggctg cctcggcttt tttctgcgct
    21721 gcggctttgg cggcttctgc ttctgctttt ttctttgcgt ctgcagccgc tttcttcgct
    21781 gcttcttccg cagctttagc atctgcttcg gccttcgctt tagcatctgc cgccgctttc
    21841 gctgccgcct cttcagcttg cttctgcttt aactcggcct gttttgcggc ttcttcagcc
    21901 tgctttttct gctcctgagc cgctaaccgc tctttctcaa gttgcttcag gcgttcctgt
    21961 tcagccgctt gtttctcacg cagttcttca gcagcctgct gttccttcat cttgcgctgt
    22021 tcatcagaac gcttcgcgct tgattcctgg ctttgcatgc gtttgtactg ctcaactacc
    22081 gcacctgaat caaccatgac agcgtcgatg gacgaaccac cgccgcctcc ggctgaagct
    22141 tctatattct catcgaacga actccagatc agcgccgcaa ataagatgac atgcagcact
    22201 gctgaaataa ttatcgcccg cttgagcttg tcgttttgtt cggttgcctt tgacactctc
    22261 ggtttccaaa aactgttcgc ctgttacccg ctctctttca agcaagggaa acgcagatgt
    22321 ttagataggc tgcgtcatta aaccaaccga tttcacaccc gcactatgta acaagttcag
    22381 tgctttaatt atttcatcgt aaggcacatc ttttgcgcca ccgatcagaa agaccgtttt
    22441 cgggttggcc ttgaagcggc tggacacttc cgccaccacc tgctctggtg gtaaacgctc
    22501 caggcgatct ttctcaacca ccacggtgta ctgaccaata ccagacactt caacaatcac
    22561 tggcggatta tcgttactgc tcaccgcctg tgattcagta gcgtctggca gatcgacctc
    22621 cacgctctgg gtgatgatgg gcgctgtcgc catgaagatc agcaacagca ccagcagtac
    22681 gtccagcaac ggtacaatgt tgatttcgga cttaagttcg cgacgacctc gtccacgcgc
    22741 tctggccatg acttacccct tattgctctc gctaacggta aacgcctggc ggtgcagaat
    22801 cgcggtaaac tcttccataa agttgtcgta attcagttcc agtttgttta cgcgctggtt
    22861 gaggcggttg taggccataa ctgccgggat agcggcaaac agaccaattg cagtcgcaat
    22921 caacgcttct gcgataccgg gcgcaaccat ttgcagtgtt gcttgtttta ccgccccgag
    22981 ggcgataaag gcatgcatga tcccccagac cgtaccaaac agaccaatat acgggctgat
    23041 ggagccaacc gtaccgagga acggaatgtg cgtttccaga ttttcaagtt cacggttcat
    23101 ggagatacgc atagcacgcg acgccccttc cactacggct tccggcgcat ggctattggc
    23161 acgatgcagg cgcacaaact ctttgaaccc gctgtaaaat atttgttccg aaccagtcag
    23221 attatcccgt ttcccctgac tctcttgata gaggcgagag agttcgattc cagaccagaa
    23281 tttatcttca aacgcttcgg cttcgcgcgc cgctgcgtta agaatacggg tccgctggat
    23341 aataatggcc caggatgcga ttgaaaaacc aatcaaaatc aacatgataa gtttaaccag
    23401 aaggctagcc ttcaggaaca aatcaaggat attcatgtca gtcactgctt aaactccgcg
    23461 acaatagact tgggaagcgc acgaggcttc attttgagtg ggtcaacgca aacaaccaga
    23521 acctctgctt cattcagcaa agtattctcg gcgttgacaa tacgttgcgt gaaaaccaaa
    23581 gaggtgccac gcattgatgt tatttcagtc tgtatttcga gcatatcgtc gagccgcgca
    23641 ggtgcgtaat attccaccgt cattttacgt accacaaagg caacgcgttc agccatcagc
    23701 gcctgctgac tgaagtgatg atgacgcagc atctctgtgc gtgctctttc ataaaaagcg
    23761 acgtaactgg cgtggtacac cacaccaccg gcatcggtat cttcatagta gacgcgagcc
    23821 ggccatcgaa acagcgttgt attcacttta catcccggta atgcaacaaa agttagagct
    23881 tttaaacgct gctactatac gcgcgcgatt gaggtttggg aatgagggca agttaaggga
    23941 gcgtaaaaat ttatgggcct ggagcggccc ataatttttt aaagagaatt ctgatttaaa
    24001 agaagaagaa aatcagcccg ataatgagaa caatatcggc aagcaatggg caaaaaatcc
    24061 cttgccaaag aaccttctgc ggacgaaagc ccacgccgtg aatcacacca gcacagacgg
    24121 cccacatcag caataaacca tgccagattt ccagttcact ggtcttcgcg gcaaaacgag
    24181 atgggtccca aaacatacat cctgctaaca gaagcgccat cacgaaggaa agcgcccgca
    24241 gggggcgctt gtccattacc gcatacaagg tcgcgataat cttactcatc agatgtcttc
    24301 ttgaccggct ttgcctgatt cgacgtgttc aagcgccagc gcggtgatta ccccaaacga
    24361 acaggcaaga agcgttccca gaatccatgc gaaataccac atttttagct ccttacttag
    24421 tacagagagt gggtgttacg ttcaatatct tctttggtga tacgaccgaa cattttccag
    24481 taacaccagg cggtgtagag cagaatgatc ggtaccagaa ccaccgcaac ccaggtcatg
    24541 acgttaagcg tcagctggct ggaagttgca tcccacattg tcagacttgc gttcatcatg
    24601 gtgctggacg gcatcacaaa cgggaacatt gcgataccgg ctgtcaggat gatgcaggcc
    24661 agcgtcaggg aggagaacac aaacgcccac gcggctttat ccatacgtgc agtcaggatg
    24721 gtcagcagcg gcagaaccac acccagtgcc ggaatagccc acagaattgg cgtgttgttg
    24781 aagttaacca gccatgcgcc agcttcacga accacttctt tattcagtgg gttagaggct
    24841 gcgtaatggt ccattgtcga tttcacgaca taaccatcga taccgtacat cacccatacg
    24901 ccagccagtg cgaaacagac cagtgtcacc agcgcagcca cctgagccgt tgcacgggta
    24961 cgcaggtgca gttcgcccac ggtacgcatt tgcagatagg ttgcgccctg agtaatgatc
    25021 atccctacgc tcaccacgcc tgccagcagg ccgaacgggt taagcaactg gaagaagtta
    25081 ccggtgtagt acagacgcag atattcatca acgttgaacg gtacgccctg caacaggtta
    25141 ccgaacgcaa caccaattac cagcggcgga acgaagctac caatgaagat gccccagtcc
    25201 cacatgttac gccagcgggt ttcttcaatc ttggagcggt agtcaaaacc gaccggacgg
    25261 aagaacaaag acgccagcac gaggatcatc gccacataga agccggagaa cgcagcggca
    25321 tagaccatcg gccaggcagc aaagagtgcg ccgcccgcgg tgatcagcca aacctggtta
    25381 ccgtcccagt gtggtgcaat ggagttaatc ataattcgac gctcggtgtc gttacgaccg
    25441 aggaaacggg tgagcatgcc cacccccatg tcgaaaccgt cagtgactgc aaaaccaatc
    25501 agcagaacgc caaccagcag ccaccagata aaacgcaata cttcataatc gatcatttga
    25561 cgactcctgt cttagcgtgc cggctgagta gtcgtggaag actgctcaaa gtgatagcga
    25621 ccggttttca ggctgcttgg gccgaggcgt gcaaatttga acattaagaa caattctgcc
    25681 accaggaaca gggtatacag gccgcaaatc agcaccattg agaagatgag atcgcctgcg
    25741 gtcagtgacg aattcgccac agctgtcggc agcacttcac cgatagccca cggttggcgg
    25801 ccatattcag ccacgaacca gcccgcttct acagcaatcc acggcagcgg aataccgtac
    25861 agcgcggcgc gcagcagcca ttttttctcg ccaatgcggt tgcggatgac actccagaaa
    25921 gagagcgcga tgattgccag cagcaggaag ccacacgcca ccataatacg gaacgcgaag
    25981 tacagcggcg ctacacgcgg aatggagtct ttagttgcct gttgaatctg cgcttcagtc
    26041 gcatcagcca cgtttggcgt atagcgtttc agcagcagac cgtaaccgag gtctttcttc
    26101 atgctattga actggtcacg aaccgcctgg tcggtagaac cagaacgcag ttgttcgagc
    26161 agagagtacg ccttcatccc gttacgaatg cgttcttcat gctgcaccat cagctctttc
    26221 aggccgataa ccggggtatc cacggaacgc gttgcaatga tgcccagtgc gtaagggatc
    26281 tgaatcgcaa atttgttcgt ctcctcttcc tgatcaggaa tgccgaacag agtaaaggca
    26341 gcaggcgcag gttgcgtttc ccactcggct tcaatagcag ccagtttggt tttctgcacg
    26401 tcgcccattt cgtagccgga ttcatcaccc agaacaataa cagacagaac agcagccata
    26461 ccgaagctgg cagcgatagc aaaggagcgt ttagcgaagg cgaagtcacg acctttcagc
    26521 atataccatg cgctgatacc gaggatgaac atcgcgccag tcacataacc agacgctaca
    26581 gtgtgaacga atttcacctg agcaaccggg ttaagcacca gctcggagaa gctcaccatc
    26641 tccatacgca tagtttcaaa gttgaaatcg gacgcgattg ggttttgcat ccagccgttc
    26701 gcaaccagaa tccacagtgc ggacaggttt gaaccgagcg ccaccagcca ggtgacacac
    26761 atatgctgaa ctttacccag acgatcccaa ccgaagaaga acagacctac aaaggtggat
    26821 tcgaggaaga aggccatcag accttcgatt gccagcggcg caccgaagat atcccctaca
    26881 tagtgggaat agtaagacca gttagtcccg aactggaact ccatggtcag accggtagcc
    26941 acacccagag cgaagttgat accaaacaac ttgccccaga acttggtcat atctttataa
    27001 atctgtttgc cggagaggac gtagaccgtt tccataatgg ccagcaggaa cgccataccg
    27061 agcgtcagtg gcacaaaaag gaagtggtac atcgcggtca aggcaaactg taagcgcgac
    27121 agttcgacta tatctaacat catgactcct tgctcatcgc atgaagactc cgagagtgaa
    27181 ccccgttaga aagggtcaca cgcatgcccc aatagaaatt tgtttgaaca ctttcatcat
    27241 accatgtgaa tgcacacaaa aacggcgtaa agaaaggttg caaacatgtt aataaaaact
    27301 caaattgatc ccacgtatat attacgccgc aaaatcctta caataaacag gtttttgttg
    27361 aagcaaattt gcatttttcg acggtgatca atttatagct aaattaccgc ctttcagcca
    27421 atttgatcga gaacaattta tctctttttg atgcccattt ccaagactta tacattgata
    27481 aatatcaatt cctacaaatt atgttaatca tgtttcaata aggtaacttt agtgacattt
    27541 atgtttaaaa tgtgtgagtt ataaggctga aattagtttc ggttggcaat taaagataaa
    27601 aagagacgaa gcaaaaacag cacggttgac cttaattctt tttttggtca ctggctaata
    27661 cgacgcagag tttcaggatt actaataaag gcataaatcc acccccgccg atattaacat
    27721 ttaattaact tataattata ttataattaa ctcacccagg gttattattt tgttaaaaat
    27781 aaagccacat atacgtggct tgagagtgaa gataaacgca aaattacctg atgcgctacg
    27841 cttgccagtg tctacatcat ctctgcaata tattgaattt gcctgctttt gtagaccgaa
    27901 taaagcgttc atgccgtatc cggcatgaac aaagcgccct ttgtcaacaa tctggccgcg
    27961 catatgtggc ttcggtttga ttgctattca ggcaagccgg taactgaacg tccgtgactg
    28021 gccgggtaaa aaaggccccg atagatttga accttgattt tcctcggtgg taatgtgttc
    28081 atccatcatc gtttctgagc aagaaatcac ctcgcgactg aaagcaacag tcgcatcaca
    28141 ggttgctgat tcagccggat taaacagccg taaaatcact tcttgtcggt cttcagcttt
    28201 cttaagtgcg cttatcaggc atcccactgg gggcattttc aacaaactat aactttccgg
    28261 cacgttgaat ccggctttgt tgagcttcat cgcatcccat gggattttgt tgtaacactg
    28321 tactggagtc agccatgctc gcgcctgctg agctacacca gcggcggttg gcgtaccggt
    28381 ataactcaat aaacttaggc gacaagaaag cagaccacgt agttgtgagt ccgggactgg
    28441 cattttaatt cccgaaggcc gcccaggcct taaaagcaga tcttctttgc ccagtaagcc
    28501 cacgccacgc agcaacgtaa tggcaaaggt tttcttctct tcaccgatga cttcaaattc
    28561 acgtaaccct tcgctaaaga cagccatgcc gttacgccct tcctgtaagg caacatagtt
    28621 gagcatattc cataccggaa ccggcgcttc tttccagcct tcttgctgcc agttgttcat
    28681 tgcactgtcg ttcacggggc gcgttagcga accaaactgc gtatctgcca gaacactgtc
    28741 ggtgttaaaa ggtgtaggga ccaggacacg aaggcgatga tcgtcagcct ggttatcaag
    28801 gttgatatcc acatcaatac gcctgctgtt atgactaaga gtgacaacca acaccacccc
    28861 ta
//
LOCUS       DAJTNN010000032        28073 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTNN010000032 DAJTNN010000000
VERSION     DAJTNN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.032"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..364
                     /locus_tag="OO150_001274"
     CDS             <1..364
                     /locus_tag="OO150_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5515478.1"
                     /translation="GPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLL
                     GISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWA
                     ITAHDRKYDRNHVSIRPY"
     gene            1075..4260
                     /gene="rne"
                     /locus_tag="OO150_001275"
     CDS             1075..4260
                     /gene="rne"
                     /locus_tag="OO150_001275"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5515479.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(4457..5410)
                     /gene="flgL"
                     /locus_tag="OO150_001276"
     CDS             complement(4457..5410)
                     /gene="flgL"
                     /locus_tag="OO150_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR5515480.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFAIQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLAVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(5422..7065)
                     /gene="flgK"
                     /locus_tag="OO150_001277"
     CDS             complement(5422..7065)
                     /gene="flgK"
                     /locus_tag="OO150_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR5515481.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(7131..8072)
                     /gene="flgJ"
                     /locus_tag="OO150_001278"
                     /pseudo
     CDS             complement(7131..8072)
                     /gene="flgJ"
                     /locus_tag="OO150_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            complement(8072..9169)
                     /gene="flgI"
                     /locus_tag="OO150_001279"
     CDS             complement(8072..9169)
                     /gene="flgI"
                     /locus_tag="OO150_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5515482.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVSGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(9181..9879)
                     /gene="flgH"
                     /locus_tag="OO150_001280"
     CDS             complement(9181..9879)
                     /gene="flgH"
                     /locus_tag="OO150_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5515483.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(9932..10714)
                     /gene="flgG"
                     /locus_tag="OO150_001281"
     CDS             complement(9932..10714)
                     /gene="flgG"
                     /locus_tag="OO150_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5515484.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMQQKLTQL"
     gene            complement(10852..11607)
                     /gene="flgF"
                     /locus_tag="OO150_001282"
     CDS             complement(10852..11607)
                     /gene="flgF"
                     /locus_tag="OO150_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR5515485.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(11627..12835)
                     /gene="flgE"
                     /locus_tag="OO150_001283"
     CDS             complement(11627..12835)
                     /gene="flgE"
                     /locus_tag="OO150_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5515486.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(12860..13555)
                     /gene="flgD"
                     /locus_tag="OO150_001284"
     CDS             complement(12860..13555)
                     /gene="flgD"
                     /locus_tag="OO150_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5515487.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTMLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAITASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(13567..13971)
                     /gene="flgC"
                     /locus_tag="OO150_001285"
     CDS             complement(13567..13971)
                     /gene="flgC"
                     /locus_tag="OO150_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR5515488.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(13975..14304)
                     /gene="flgB"
                     /locus_tag="OO150_001286"
     CDS             complement(13975..14304)
                     /gene="flgB"
                     /locus_tag="OO150_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5515489.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTLGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYQMSLSALSGQIKGMM
                     NVLQSGN"
     gene            14460..15119
                     /gene="flgA"
                     /locus_tag="OO150_001287"
     CDS             14460..15119
                     /gene="flgA"
                     /locus_tag="OO150_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5515490.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARMRMVSGLVVSGVVDADGNILIN
                     L"
     gene            15195..15518
                     /gene="flgM"
                     /locus_tag="OO150_001288"
     CDS             15195..15518
                     /gene="flgM"
                     /locus_tag="OO150_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5515491.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSTS
                     TSTSTSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADALINEAQQ
                     DLQSN"
     gene            15523..15939
                     /gene="flgN"
                     /locus_tag="OO150_001289"
     CDS             15523..15939
                     /gene="flgN"
                     /locus_tag="OO150_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5515492.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(15979..17514)
                     /gene="murJ"
                     /locus_tag="OO150_001290"
     CDS             complement(15979..17514)
                     /gene="murJ"
                     /locus_tag="OO150_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5515493.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(17681..18547)
                     /locus_tag="OO150_001291"
     CDS             complement(17681..18547)
                     /locus_tag="OO150_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5515494.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAAPDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(18549..19196)
                     /locus_tag="OO150_001292"
     CDS             complement(18549..19196)
                     /locus_tag="OO150_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5515495.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAELESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(19207..19791)
                     /gene="rimJ"
                     /locus_tag="OO150_001293"
     CDS             complement(19207..19791)
                     /gene="rimJ"
                     /locus_tag="OO150_001293"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5515496.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            20027..21235
                     /gene="mdtH"
                     /locus_tag="OO150_001294"
     CDS             20027..21235
                     /gene="mdtH"
                     /locus_tag="OO150_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5515497.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            21299..21946
                     /gene="grxB"
                     /locus_tag="OO150_001295"
     CDS             21299..21946
                     /gene="grxB"
                     /locus_tag="OO150_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5515498.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            22080..22640
                     /locus_tag="OO150_001296"
     CDS             22080..22640
                     /locus_tag="OO150_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5515499.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            22746..23792
                     /gene="pyrC"
                     /locus_tag="OO150_001297"
     CDS             22746..23792
                     /gene="pyrC"
                     /locus_tag="OO150_001297"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5515500.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            23866..24111
                     /gene="dinI"
                     /locus_tag="OO150_001298"
     CDS             23866..24111
                     /gene="dinI"
                     /locus_tag="OO150_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5515501.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            24401..24655
                     /gene="bssS"
                     /locus_tag="OO150_001299"
     CDS             24401..24655
                     /gene="bssS"
                     /locus_tag="OO150_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5515502.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            24770..25888
                     /gene="solA"
                     /locus_tag="OO150_001300"
     CDS             24770..25888
                     /gene="solA"
                     /locus_tag="OO150_001300"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5515503.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCTGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            25936..26049
                     /locus_tag="OO150_001301"
     CDS             25936..26049
                     /locus_tag="OO150_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5515504.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            26310..26876
                     /locus_tag="OO150_001302"
     CDS             26310..26876
                     /locus_tag="OO150_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5515505.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFTLGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            26880..27455
                     /locus_tag="OO150_001303"
     CDS             26880..27455
                     /locus_tag="OO150_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5515506.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGNDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(27497..>28073)
                     /locus_tag="OO150_001304"
     CDS             complement(27497..>28073)
                     /locus_tag="OO150_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5515507.1"
                     /translation="EYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGG
                     IRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISD
                     EIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQR
                     RRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
BASE COUNT         6772 a         7449 c         7084 g         6768 t
ORIGIN      
        1 tggaccactt attgcgacgg cagcggtggc caccatgggg gaagcatcag cgtttaaatc
       61 ggggcgagag ttcgccgcat atgttggtct ggttccaaaa caaactggct ccggagggaa
      121 agtacgtctg ctggggataa gcaaacgtgg tgacacttat ctcaggacat tatttatcca
      181 cggtgcaaga gcggtggcat tagtagctaa agagcctggc ccgtggataa ccgaactgaa
      241 aaaacgtcgt ccagccagtg tggcaatcgt cgccatggca aacaagctgg cacgaacagt
      301 atgggcgata accgcccatg accgtaagta tgacaggaac cacgtcagta tcagaccata
      361 ttaatcgctg ataccattaa acaatgaact cttaacaaaa gggtgaatgc tgaaaggttg
      421 ctatggcggc cagagtgatg acaaagacag gtaagaccgt gactcactaa acctgaacag
      481 tattttgggc ttgaagtccg ccgtgaaaat aaggggtgag tcggcgaatt acataggggc
      541 tcgcagcgtt acggctgcaa taaagccgga tataaagctg caacctaccc gtcatgtcaa
      601 aacaatggat gccttgcaaa cgggatgcgt tcatataaaa actgtgagta agcgggtgat
      661 aaatggtaaa agtcatcttg ctataacaag gcttgcagtg gaataatgaa gccgtttccg
      721 tgtccatcct tgttaaaaca agaaatttta cggaataacc cattttgccc gaccgatcat
      781 ccacgcagca atggcgtaag acgtattgat ctttcaggca gttagcgggc tgcgggttgc
      841 agtccttacc ggtagatgga aatatttctg gagagtaata cccagtctgt ttctttgata
      901 attgcgctgt ttttccgcat gaaaaacggg caaccgacac tctgcgcctc tttgagctga
      961 cgataaccgt gaggttggcg acgcgactag acacgaggcc atcggttcac acccggaaag
     1021 gcgttacttt gcccgcagct tagtcgtcaa tgtaagaata atgagtaagt tacgatgaaa
     1081 agaatgttaa tcaacgcaac tcagcaggaa gagttgcgcg ttgcccttgt agatgggcag
     1141 cgtctgtatg acctggatat cgaaagtcca gggcacgagc agaaaaaggc aaacatctac
     1201 aaaggtaaaa tcacccgcat tgaaccgagt ctggaagctg cttttgttga ttacggcgct
     1261 gaacgtcacg gtttcctccc actaaaagaa attgcccgcg aatatttccc tgctaactac
     1321 agtgctcatg gtcgtcccaa cattaaagat gtgttgcgtg aaggtcagga agtcattgtt
     1381 cagatcgata aagaagagcg cggcaacaaa ggcgcggcat taaccacctt tatcagtctg
     1441 gcgggtagct atctggttct gatgccgaac aacccgcgcg cgggtggcat ttctcgccgt
     1501 atcgaaggcg acgaccgtac cgaattaaaa gaagcactgg caagccttga actgccggaa
     1561 ggcatggggc ttatcgtgcg caccgctggc gtcggcaaat ctgctgaggc gctgcaatgg
     1621 gatttaagct tccgtctgaa acactgggaa gccatcaaaa aagccgctga aagccgcccg
     1681 gccccgttcc tgattcatca ggagagcaac gtaatcgttc gcgctttccg cgattactta
     1741 cgccaggaca tcggcgaaat ccttatcgat aacccgaaag tgctcgaact ggcacgtcag
     1801 catatcgctg cattaggtcg cccggatttc agcagcaaaa tcaaactgta caccggcgag
     1861 atcccgctgt tcagccacta ccagatcgag tcgcagatcg agtccgcctt ccagcgtgaa
     1921 gtccgtctgc cgtccggtgg ttcgattgtt atcgacagta ccgaagcgtt aactgccatc
     1981 gacatcaact ccgcacgcgc gacccgcggc ggcgatatcg aagaaaccgc gtttaacacc
     2041 aacctcgaag ctgccgatga gattgctcgt cagctgcgcc tgcgtgacct cggcggcctg
     2101 attgttatcg acttcatcga catgacgcct gtacgccacc agcgtgcggt agaaaaccgt
     2161 ctgcgtgaag cggtgcgtca ggaccgtgcg cgtattcaaa tcagccatat ttctcgcttt
     2221 ggcctgctgg aaatgtcccg tcagcgcctg agcccatcac tgggtgaatc cagtcatcac
     2281 gtttgtccgc gttgttctgg tactggcacc gtgcgtgaca acgaatcgct gtcgctctct
     2341 attctgcgtc tgatcgaaga agaagcgctg aaagagaaca cccaggaagt tcacgccatt
     2401 gttcctgtac caatcgcttc ttacctgctg aatgaaaaac gttctgcggt aaatgccatt
     2461 gaaactcgtc aggacggtgt gcgctgcgtg attgtgccaa acgatcagat ggaaaccccg
     2521 cactaccacg tgctgcgcgt gcgtaaaggg gaagaaaccc caaccttaag ctacatgctg
     2581 ccgaagctgc atgaagaagc gatggcgctg ccgtctgaag aagagttcgc tgaacgtaag
     2641 cgtccggaac aacctgcgct ggcaaccttt gccatgccgg atgtgccgcc agcgccaacg
     2701 ccagctgaac ctgccgcgcc tgttgtagct ccagcaccga aagctgcacc ggcaacacca
     2761 gcagctcctg cacaacctgg gctgttgagc cgcttcttcg gcgcactgaa agcgctgttc
     2821 agcggtagtg aagaaaccaa accgaccgag caaccagcac cgaaagcaga agcgaaaccg
     2881 gaacgtcaac aggatcgtcg caagcctcgt cagaacaacc gccgtgaccg taatgagcgc
     2941 cgcgacaccc gtagtgaacg tactgaaggc agcgataatc gcgaagaaaa ccgtcgtaat
     3001 cgtcgccagg cacagcagca gactgccgag acgcgtgaga gccgtcagca agctgaggta
     3061 acggaaaaag cacgtaccac cgacgagcag caagcgccgc gtcgtgaacg tagccgccgc
     3121 cgtaacgatg ataaacgtca ggcgcaacaa gaagcgaagg cgctgaatgt tgaagagcaa
     3181 tctgttcagg aaaccgaaca ggaagaacgt gtacgtccgg ttcagccgcg tcgtaaacag
     3241 cgtcagctca atcagaaagt gcgttacgag caaagcgtag ccgaagaagc ggtagtcgca
     3301 ccggtggttg aagaaactgc cgctgccgaa ccaattgttc aggaagctcc agctccacgc
     3361 acagaactgg tgaaagtccc gctgccagtc gtagcgcaaa ctgcaccaga acagcaagaa
     3421 gagaacaatg ctgataaccg tgacaacggt ggcatgccgc gtcgttctcg ccgctcgcct
     3481 cgtcacctgc gcgtgagtgg tcagcgtcgt cgtcgctatc gtgacgagcg ttatccaacc
     3541 cagtcgccaa tgccgttgac cgtagcgtgc gcgtctccgg aactggcctc tggcaaagtc
     3601 tggatccgct atccaattgt acgtccgcaa gatgtacaag ttgaagagca gcgcgaacag
     3661 gaagaagtac aagtgcagcc gatggtgact gaggtccctg tcgccgccgc tgtcgaaccg
     3721 gttgttagcg cgccagttgt tgaagaaatg gctgaagtcg tagaagcccc cgtaccggtt
     3781 gccgaaccgc aaccggaagt ggttgaaacg acgcatcctg aagtgattgc cgccgcggta
     3841 actgaacagc cgcaggtgat taccgagtct gatgttgccg tagcccagga agttgcagaa
     3901 cacgcagaac cggtggttga accgcaggaa gagacggcag atattgaaga agttgccgaa
     3961 actgctgagg ttgtggttgc agaacctgaa gttgttgctc aacctgccgc gccagtcgtc
     4021 gctgaagtcg cagcagaagt tgaaacggta gccgcggttg aaccagagat caccgttgag
     4081 cataaccacg ctaccgcgcc aatgacgcgc gctccggcac cggaatatgt tccggaggca
     4141 ccgcatcaca gtgactggca gcgccctact tttgccttcg aaggtaaagg tgccgcaggt
     4201 ggtcatacgg caacgcatca tgcctctgcc gctcctgcgc gtccgcaacc tgttgagtaa
     4261 taattagctc aaagtaatca agccctggta actgccaggg cttttttatt tcatgtttga
     4321 atcaacatat taaaattcat caaatggcat ccttgctaac caacaatgca aaataggcaa
     4381 aaaaaatgcc cgccccggcg atccctgagc gggcagacaa aacataccca gtttcatgat
     4441 atgttttaaa gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa
     4501 atgctttata cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat
     4561 tccagtccac atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac
     4621 ctaaggaatc cagcgactcc agttcgttca gctgcgtgcc taattccgcg cgaacggcaa
     4681 ggacattatt cagcgagttt ttcagtccac ggttggtttt atccaatgcc gcagcggcga
     4741 tttctttatc cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca
     4801 gcatggcaaa aagattggtt tcagaagcgc taccgtctgg ctccgcaact gcgttgctgg
     4861 taatactgtc gaaaattttg tcacccgtgt gccctatcac catcgaacgc gaagcatcga
     4921 cctgttgctt aatactttct gctccaccga cgtatttccc tttctcttcg ctaaacggcg
     4981 cagtctctgt tttataaccg gcaaaaatgt agcgcccgtt accgtcagtg gtgttcgcca
     5041 gattcagcaa ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt
     5101 cactcaaggt gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg
     5161 tgacctggct aagtacactc tcttccagtg acactttttg aatggcgaaa gtacgcgcca
     5221 gcgtgtactg gctgttttgc gcctgtgcct gggagagaac tacggcttgt gatgctgcaa
     5281 tgggatcgtc agaagggtta acgactcgct tacctgtcga catctgctcg ccgtacttca
     5341 tccattccgc ctgagaattg gtgatgccac gcatgttttg ctggtacatc atctgggtac
     5401 tgaaacgcat cttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc
     5461 cgtctgcaga acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta
     5521 ctcttcgtcg agattgacgc cagagataga ctgctgctga ttggaaagct gcgtcaccac
     5581 attaccttgc gtggcgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac
     5641 taacgaggca taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg
     5701 cagatccagc agggcctgac cgttgcggtt atcgctatca cccgcatctt tttcgctcgc
     5761 catcgctatt ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac
     5821 tggtttcagc gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc
     5881 atcaaatgcc actttaccgt tggcatccgg cgtcaccgta aaagtggtat tgctggcaag
     5941 gcgggtgacc tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccgc
     6001 ggaggcatca gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg tgttttgcaa
     6061 aaccgcgggc ttaccgatag caaagaaatc ttcaccagca tcgccgttgg catcaaatcc
     6121 ggctttgtgt tgggtgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt
     6181 acgcgtctgg tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt
     6241 cagtaatttc tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt
     6301 acgagaaggg tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac
     6361 cagtgagtaa ccattggcca tcgtgatgtt ataagtgccg ccatcctgaa cgctgacttc
     6421 tacaccaaca atctggttta attcgctcac cagttgatcg cgttgatcca gcagattgtt
     6481 aggtgacgcc cctgccccca cgcctgtcag gcgggagatt tggtcgttca ggctggcgat
     6541 ttgtttagcg taattgttga tctgatcaac gctggcacct atcgcgatat tgacctgttt
     6601 gtcctggtca cgaagatact gatcggtggt tttaaactga ttcaccaacc cttctgattt
     6661 cccaatcagc gcctggcgcg ctgccgggtc ttccgcgtta ctcaccagcg tttgcaggct
     6721 ggtgaagaaa tcctgcatct gtgttgccag cgaagaggta ctggtggaga gcatattgtc
     6781 gattttcgac atctgctcat agcgggcagt caggccgcta ctttgcgtct gcgccgcacg
     6841 taactggttg gtgataaacg catcatactc acgctgcaca ccagaaacgt agacaccatt
     6901 gccaacccag ccgccagcgc ccaacgtgct attggcctgc gccataatag tggtttggcg
     6961 ggtatatccg gcaacgttat agctggagat attattactt gccgtattta acgccgcctg
     7021 ggccgcgttc agtccgctca tggcgttatt aatcaagctg gacatggagg ttccttataa
     7081 gccttcaatt acagagtatt atcggcagcg acccgccgga cttgagttat tcaaaacaga
     7141 ttatcaatgt tcatactgta ggttttgctc accttgtcgc ttatcgattt catctgctga
     7201 atcatgttgg tgagtttgcg ggcatagtga ggatcggtgg catagcccgc gtcctgtagg
     7261 gcctgcgccc cctgctccgc actcgtggcg gtggtcacgg cggcgtagcg cgggttacgc
     7321 gttaacagcc caacgtaatc cgactatgct tccagatacg agctgtagac ccgaaacttc
     7381 gcttttactt tcttcgcttc gccgttttca tattcagtcg tggtgatttc agtaactggc
     7441 cctttccagt tgccagaggc tttgacacca aacaggttat agctcggctc gccgttttcg
     7501 cggcggattt gccgttgccc ccaaccagat tccagcgccg cctgagcgag gatcaaatga
     7561 tgtggcacac cgctttgctg gcttgccagt tgggcgggca gcgagagttg cgcgaggaat
     7621 gctttactgt cacccggcag cgaatcatcg tagttacgtg gcacggcctt ttgcaccagc
     7681 tgcgaaagcg cctgattttg ataacgcacc acagtttcga gcgggaattt catcggtgct
     7741 gctggcgtgg actcctctgg caatggttgt tctggcgtca tctgtttaac catcatctct
     7801 gcaagcccca gacctttgcc cgccgtcatc tgttgggcaa tctgctggtc atacatactg
     7861 gtatacaggc gagtgtgctc gctgctgaac aggccatctt ttggtaaagc gtcgcgcatg
     7921 cttttcaaca tcatctgcac gaacatccct tccacctgac gggccaccgg acggatattt
     7981 gccgccggat cttcgcccgc tttcgccttt agttcgttga gtgattgcgc atcccaggcc
     8041 gcacttgcca gtagtttgct atcgctgatc atcagatgat ttccagtttt gcccgcagac
     8101 atcccgcact ttgcattgat tgcagtatgg acatcagatc catcggcgta gcgcccagcg
     8161 cattgagcgc gcgcaccacg ttattgagcc tggcgctgga acgtacgctt tgcagcgaac
     8221 cgccgctctg gcgtaaatcg atctgcgttt gtggagtaac cacagtctgt ccaccaccaa
     8281 acggtgtatc tggctggctg acattggcct gacgattaac tgttactgag agattcccct
     8341 gcgctaccgc gcagctgtcg agggtcactt cgcgattcat caccaccgaa ccggtgcgcg
     8401 agttaatcac tactttagcg tcctgcgggg tgacattaac ctgcatattc tggatatcgg
     8461 caaggaagcg gacctgggaa ctgttgccac tcggtacgcg cacctgaata gtccgcgcat
     8521 ctaacgcggt ggcgctgcca tatccacgca cgcggttgat ggtgtcagcg atttgctgcg
     8581 ccatgctgaa atcttcgtcg ttaagttgca aattaagggt attcccgacg ccaaactggc
     8641 tgggcaattc acgttcaata accgcaccat tggtgatccg tccaccgttc agttggttaa
     8701 cctgaacact gctaccgcca gcggaggctc ctgcgccgct aaccagaata ttgccctgcg
     8761 ccagcgcata cacctgactg tcaacgccct taagcggtgt catcaacaac gtacctccac
     8821 gcaagctttt ggcattaccc atggaagaaa ccaccacatc gatggtttgc ccctgacgtc
     8881 caaacggagg aagtgacgct gtcaccatta ccgcagcgac gttttttagc tgcatattgg
     8941 tgcccgtcgg aacggtaatt cccagctgtg agagcatgtt attaagcgtt tgtgtggtaa
     9001 acggcgtctg ggttgtctgg tcaccggtgc catccagccc caccaccaga ccatagccaa
     9061 tcagtgagtt ttgccttacc ccctgaacac tggtgagatc gcgaatacgc tcagcctgag
     9121 ccgccgtcgt gaccagtaga agaattaatg cagagagaaa tttaatcacc acagcctcac
     9181 ttacattggc gacaggttaa ggaagaaacg ctgcaaccag cccatatttt gcgcttcgtt
     9241 aatgtagcca ttgcctacgt attcaatgcg cgcatccacc acctgagtag acggtacggt
     9301 attgctgccg ctgatagtgc gtggattaac cacgccagag aagcgaataa attcggtacc
     9361 ctgattaatg gcaatctgtt tttcacccac cacatgcagg ttgccgttga ccagtacctg
     9421 gtcaaccgtc accgtcaacg tgccgctaaa ggtattgctg gcattggccc cgccctttcc
     9481 gttgaacgtg ttaccaccgg aggcttcgac atcggcacga gcgttaccaa acagcccctg
     9541 caaatagcgc ggcacagtat caaagccaaa attagtttta ccgtcacggc tggcattcgc
     9601 agaggagctt ttgctggcgc tgacgttctc ctgcaacacg atggtcagcg tatcgccaat
     9661 attgcgtggt cgacgatctt caaacagcgg ttgatagcca tagttaatcg gctgagcaga
     9721 ctggaaaata gaaccgttgg cgacgggcgt cggaccggga accggttgtg cactggtcgc
     9781 cccctgcacc agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa gcaccaacaa
     9841 gctggaaatg gcataagtat gcgcagcgtt tttttgcatg gctatcatct tcaaaaatca
     9901 gtaaaccggt gaacctgcca ccggttaagc cttagagttg cgtcagtttt tgctgcatct
     9961 gatcggtggt ggacaccgct ttactgttga tttcgtaagc gcgttgcacc tgaatcatat
    10021 tgaccagttc ttccgccacg ttgacgttag acgtttcaac atacccttga tacagcaatc
    10081 ccgcgccgtt caggcccggc gtgctttcgt tcggtgcacc agaggattgc gtttcggtgt
    10141 agaggttttc gccaatgctc tccagcccgg tgtcattcat aaaggtggtg agattgagct
    10201 gcccaacctg aaccggagct gcctggcctt gttgggttac gctgaccacg ccatcacgac
    10261 cgatggtgat acttaacgca ttcgccggaa tggtgatcgc tggctgcacc tgaaaaccac
    10321 cagccgtcac cagctgcccg ttctgatcca cctggaaaga gccgtcacgg gtataggctg
    10381 atgaaccatc tggcaacatc acctggaaaa agccctgccc tttaatcgcg acatctttgc
    10441 tgttgttggt ctgcgacaga tttccctggc tgtgtaagcg ttcagtggcg accgggcgta
    10501 cccccgtgcc gatttgtaat ccggagggta aggtggtttg ttcggaagac tgtgcccccg
    10561 gctggcgaat ggtttgataa agcagatctt caaacaccgc gcgctgacgc ttaaaaccgt
    10621 tagtactgac gtttgccagg ttgttggcaa tgacgtccat attggtttgc tgggcgtcaa
    10681 ggcccgtttt ggcgatccat aatgaactga tcataaaatg tcctgtagtg gattagtgac
    10741 aagcagagca gaagcacgcg acagtgtgaa ttccccctca ctctaaccct ctccccaaag
    10801 gggcgaggga atagtccgcg cgcttttatt gcaatgtcat caatcgttca attaactcat
    10861 cgacagcagt tggttggcac ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg
    10921 catttcaaaa cgccgcgcgc tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt
    10981 actgccttcc agaacccccg acatcacacg caaggttgga tctgcctgca gtaccggccc
    11041 tcgcgtggcc tgggtttctg cgcttaaacg aaaaatgccg tcgtcaccgc gctgcacttc
    11101 gctgcccgtg gctttcacca gtttcagacg ccctactggc gcaactgtat ttgccggatc
    11161 gcccggattg agcgccgaga ttgtgccatc ggcagcaata gtgatttccg ctccttccgg
    11221 cacagcaatt ggccccgcct cgcctatcac ctgatgcccc tgaattgtca gttgcccggt
    11281 gggatcaacc tgaatgctgc cattacgcgt atacccttcg ctgccgtcag cggtctgcac
    11341 ggccagccag ccatcctgct gcaacgcaac gtccagcggg cgcgaggtgt aatctatctt
    11401 gccgggcgtc atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga
    11461 aagcccttcc accggcactg cgcgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga
    11521 ggcattggcc agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc
    11581 catcgcagta taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa
    11641 ccagcgtgtt gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac
    11701 gctgggcaac gatcatattg accagttctt tactgagatc gacgttggac gcttccagcg
    11761 caccgttggt cagggtgcca aagtttcccg tcccggctgt ccccaacagc gccacgccag
    11821 aagattgcgt cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa
    11881 agttcgccag tacaatctgc cccagcagtt gagtttgttc gttggaatag ttgccgacaa
    11941 ccgtaccgtc atcattgatt tgataactca ccagatcgcc cggtttgtag ccgttctggg
    12001 gggttgccac aatattgtta gcgccggtat tttgctgcat ggagttgagg aagctcagac
    12061 taaacgtggc gggttctgca ccgttaattg cgccggttgc gatattattc gccatcgcac
    12121 catccactaa tgtgccatta gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt
    12181 ttggatcact gctatcctgg gtgtagacct gccagttatt atccccggtc ttcacaaagt
    12241 agacgctcat gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt
    12301 tatagctatc cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga tcactggaat
    12361 tcaggttgat ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggga
    12421 tcgaaatatt ggtcggattc gccccttgct gaatagtcgg cggcgtaccg gttgccgggt
    12481 aacccgtcag ctgtaaacct tgcatattca ccaggttacg gttttcatcc agcttaaatt
    12541 gtccgttacg gctgtagaac accgaaccgt tgctgtctac cagacggaaa aaaccgttct
    12601 ggctgatagc aacgtccaga cctcgcccgg tgttggtggt cgtgccatct gtaaagtcct
    12661 gagtgatacc ggcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa
    12721 aagaggccgt gcctgattta aagccgtagg tggcggagtt ggcaatattg ttgccaataa
    12781 catcgaggtt agtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg
    12841 actcctgaaa tgtgaaggct taaattatct gccgtacttc gtcgagggtg gtggtgccgt
    12901 aagtgccgag atccagcgta ttaccgctgt tgccgcggat cacaccctgc accagagcaa
    12961 actgcagcgg ctgggcaacc agttgtgtac caccgttact ggcggtaatc gctacattgt
    13021 aagaaccgtt cggcgcagtg ctgccatcag tcaacgtacc gtcccaggtg aaactgtgaa
    13081 ctccggcggt cagttcacca atatcaatgg tgcgcacaac cgcgccattt ttatcggtga
    13141 tggtggccgt aactttgtct gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg
    13201 tcacagcccc ttcttcactg ccggttcccg caagaacagt tgtgccgggg atcatcacgc
    13261 cgtgaccgat caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag
    13321 atccgagcat ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg
    13381 acgtcagctc gttgttttcc attggattgg tcgggtcctg gtttttcagc tgcgccacca
    13441 gcaaagtcag aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc gaactactgc
    13501 tggtggtact gacgccggta tttgtcggat cggtggtggt taccgcaatg gacatagctt
    13561 tctcctttat tgaccgagcg taagggtttt cagcatcatg cttttcaccg tgttgagcac
    13621 ttcaacattg gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct ctccgacaac
    13681 atcaacgttc ggcattttta cgtagccctt tgcatccgcc agcggattac ccggttcata
    13741 aaccagtttg tccggggcct gactttctat aacatcggca acctttacgc cgcctgtcgc
    13801 agcacctggt gcagcgttaa cctggaatac cacctgtttt gcccgatatg gctgtccatc
    13861 gggaccggtc acgctatcag cattcgccag attactggcc gccacgttca ggcgctggga
    13921 ctgggcagtt aacgccgacc cggcgatatc aaaaatattc agcagtgcca tccgttaatt
    13981 tccgctctgt aaaacgttca tcatgccttt gatttgcccg ctcaacgcac taaggctcat
    14041 ctggtattgc agttctgcgg taggaggcgt cagcgcctgc gccggaatgt gttgcgttga
    14101 ggtcatcgtc agagcaacca cactggttgc atcccgtcca cgttgcatga cttttttaag
    14161 ttcactggca aaatcgatat cgcgcgcctg ataaccaagg gtatcggcat tggcgatgtt
    14221 tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa
    14281 ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct
    14341 taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc
    14401 cgttgatgac cattggggac gggtacaatt cacgttgtag aaatagctgg ggggcgaaaa
    14461 tgctggcaat aaaacgtagc gtggcgatca tcgcgatact gttcagtccg ttaagtgcgg
    14521 cgagcaatct cacatcgcaa ttgcacacct tttttagcgc ccagctcgtg ggggtaagtg
    14581 atgaggttcg tgtttctatt cgtacagcac ccaatctact accgccgtgc gagcagccat
    14641 tgctttcgat gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg gcacgctgcg
    14701 gtaacgacaa acgatattta caggttaatg tgcaggccac aggaaattat gtggttgccg
    14761 cgatgcccat tgtgcgggga ggaaagctgg aagctggcaa tgtcaaacta aaacgcggac
    14821 gactggatac cctgccaccg cgtacggtgc tggatatcaa tcaacttgtt gatgccatta
    14881 gcctacgcga tctatcaccc gatcaaccaa tccaattaac ccattttcgc caggcatggc
    14941 gggtaaaagc gggacaacgc gtcaacgtga tcgccagcgg tgacgggttt agcgccaacg
    15001 cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa tgcgcggatg cgcatggtat
    15061 cgggactggt ggtcagcggc gttgttgatg cagatgggaa tattcttata aacctgtaag
    15121 ctgttaaaga ttacccgtcc cttgccgata aataagcaac acatgataaa agcgccctca
    15181 atgaggaata aaccatgagt attgatcgca cttcgcctct gaatcccgta agcaccgttc
    15241 aaccgcgcga aaccactgac gcgccggtaa cgaacacccg ggcggcaaaa acaaccgact
    15301 ccaccagcac cagcaccagc accagcacca gcaccagcac cagtgtgacg ttaagcgacg
    15361 cgcaagcaaa actgatgcaa cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt
    15421 taaaactggc gattcgtaac ggtgaactaa aaatggacat cggcaaaatt gccgatgcgc
    15481 tgatcaacga agctcagcaa gacttgcaga gtaactgatc gtatgacacg tcttgcagag
    15541 atccttgacc agatgtccgc tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag
    15601 caacatctct ctatggggca gatcaacggc agccagttgc aatggattac agaacaaaaa
    15661 agctcactgc tggcgacgct ggattacctc gaacagttac gcaagaaaga acccaataca
    15721 gcaaatagcg ttgatattag tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta
    15781 cgccaaatga atcaacataa cggttggtta ctggaaggac agattgagcg caatcaacag
    15841 gcgctggaaa tgttgaaacc gcatcaggaa ccgacgctat atggggcgaa cggtcagacc
    15901 tcaacaaccc atcgcggcgg taaaaagatt tcgatctgaa gattatctcc ggcctgcact
    15961 gcaggccgga atgcattgtt acaccgtccg gcgggcaaat tctttaactt tgaagcccag
    16021 taccgccagt gcagcgaagt acgcggcaat ccccgccagc acgaccgcca ttaaacgcag
    16081 taagcgccag ggcatggtac ccaatgacca ctccggcatg atatgtaaca tacctaaaag
    16141 cacgccagac atcaccagta ccgccaccac cagacgcaac agaaacgcca tccagccggg
    16201 ttgcggagta aagattttct gcttacgcaa ctgccagtaa agcagcgaag cattcagaca
    16261 cgccgccaga ccaatagaaa gtgacagccc ggcatgtttc aacggaccaa taaacgccag
    16321 gttcatcaat tgcgttaaaa tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc
    16381 ctggcgggaa taaaagccag gagccaacac tttcactaca atcaggccaa tcaaacccac
    16441 cgagtaggca attaacgccc gctgggtcat cagcgcatca aacgcggtaa atttaccgta
    16501 ctggaacagc gaaacggtta acggacctga aagaatgccc aacgcaaccg cactcggcag
    16561 cgccaacagg aaacaaagac gcaaccccca gtccatcaaa cggttgtatt catcatgatt
    16621 gccactggca aaacttttcg acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag
    16681 cacaccggac ggaaactcca ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga
    16741 agcaagaaac gaggcaaaaa tggtgttgat gattaaggag atctggctca cagagacgcc
    16801 aaggatcgcc ggtcccatct gtttcaccac gcgcattgct ccggcatcgt ggaagttaat
    16861 gcgcggcagg accagcatgc cgatcttctt caggtgcggt agctgataca ccagctgcag
    16921 gatgccgccg accgtaacag cccacgccag cgccagcacc ggtgggttaa agtacggtgc
    16981 ggcaaacagc gcgaaaccaa tcatgctgat gttaagcagt gttggcgcaa acgccggaat
    17041 cgagaagcgg ttccacgtat tcagaatcgc tcccaccagc gacgccaggg agatcagcaa
    17101 gatataggga aaggtaatct ttagtagctg gctggtcagg gcaaatttgt cagctgtatc
    17161 agcgaagcct ggcgcggtca ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt
    17221 cacaaccgcc agcgcaagtg tcagcaggcc agaaacataa gagacaaaga cccgcgtggc
    17281 gtcttcaccc tgcttacttt tatattccgc cagaatcggt acaaatgcct gggaaaatgc
    17341 cccttcggca aagatacggc gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc
    17401 ggttgccatc cctgcgccaa agattctggc gacaattgcg tctcgtgcga agccaagcac
    17461 acgcgaaaac atggtcatcg agctgacggc ggccagcgat tttaataaat tcatcggtgt
    17521 tctaatccag accaacgggc aaaacgcctg caagacaggc gctgaaagag gcgaatagtc
    17581 tacctggatt atggtgaatt gctaccgcca gatgttacag ggtttattca ctcatcgcat
    17641 cgcgccagat cttgtcaacg atacgttgcg ccagcacggc ctattcgccg gcggtttgcg
    17701 gaactgtctg attttgcaca cattcaatga agtgccgcgc acagccgaca aatccacgtt
    17761 gttcaagagt gctctgccaa ccaggaatcg gtttatgcac tacgccctgc ccgcgctcct
    17821 cacgccattc gcgcatatcc gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg
    17881 tttcacgctg acttccggca cggcgatgca tacaggtggt gatctgcaaa ggaccggccg
    17941 aaaaatggtg ctcggcaaac agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat
    18001 ccagagaggc tttgccgccc gacaaccaca gcgcggtatc caccacatgc agataatcat
    18061 ccagcaacgt gaaataaaga tcgtgtggcc cgacactatt gctacgatgt ttatccattc
    18121 ttagcgaagc tgcggtagcg agttgcgttt ttaactcacc gtagagtggt gcgaaacgac
    18181 ggttaaaacc gaccatcaac gtcagttttt tccgcgccgc cagttccacc agccgttcag
    18241 catcgcgcag attttctgcc agcggtttat cgacacagac atgtacaccc gcattgagta
    18301 acgtactgac cacgtcaaag tggctggcgg tgctggaatg cacaaaaacc gcatcgcaac
    18361 tggcggcaag gctggataac gaatcggcat aaggaatgcg ccagctttca caaattggca
    18421 gggctttcgc gcgcgtaggc gaccaggctc cttgtaacat ccagtcaggc gctgccgcca
    18481 gcaccggtaa ccacgctttt tgcgcaatgc cgcctaatcc cacaacgccg atacgtaatt
    18541 ttttcacttt aatctcccag atgggccagc aacgaatcaa gacgctgttt cagttctgcc
    18601 acttcgattt ccagggcttc gacgcgggcc tgtaaatcac catcaaccgc gttcgacata
    18661 tccgttaccg ccggctgatc ctctacctca ccactgaaaa gatgcatgta gcggctttcg
    18721 cgtttacccg gttcgcgggc cagacgcacc acaaaaggac catcttcgcg atttgccagt
    18781 tgttccagcg tcgactccaa ttccgccata tcgctgaatt catacattcg cgcggcgcgg
    18841 ctgcgcagtt cacccggcgt ctgggcacca cgcaataaca acgtggtgat taacgccact
    18901 tccgctgcgc tcagtttcag atcgccaaat tctgaattac aaaaacgttg ttcatatttg
    18961 gtgacccgat taccaaagcc actcaccgtg cgcagataat ggcgtttgac cagattatcc
    19021 agttgctcct gcacttcaga ttcactcaga ttcatgaccg gttcacggtt cgttttctga
    19081 ttacaggccg tgactacacc attgactgag agcgggtatt gctccggcgt ggtcacctgc
    19141 ttttccagca agcaaccaat cacgcgagct tcaagggcag taagttgata tttcatgaga
    19201 ttctccttag cggccgggcg tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg
    19261 ccattgtcca tcaatcaaca gatagtcttt cgcatagcct tctttttcaa aacccagtcg
    19321 cgccagtaaa tcaccgctgc gtttattgtg cggcatataa ttagccataa tgcgatgaat
    19381 atgttgggtg cgctgcatat aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt
    19441 gccctgccat ttttgcccaa tcgaataacc gagatagcag gcatgaaaag agccacgaac
    19501 aacattggaa aaattggcaa cgccaataat ctctttttcg tccgggtcga ataagccaaa
    19561 gtagaaagct gaaccttgtt tatgaaattc gttaatcatc cccagcctgg cctgccagcc
    19621 tgatggataa cagtggcttt cgtcgcgcac tggctcccag ggcttgagga aatggcgatt
    19681 ctctgcgtaa taatccgcaa gacgccaggc atcacgatca tgcaccagac gcacgaccag
    19741 tcggtctgtg gttaagcgca cttttggcac gttactgcga tagccaaaca ttctatacct
    19801 actccttccc gtaacgtctt taatacgctg taagtattac tatacctgcg caaccagacc
    19861 ctgtgaaaag tccaaaatgc cattttctgc attattcaca tatttgataa aaactctcta
    19921 tcaaagcgac gttttgataa aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg
    19981 caatgcagaa tagaaagtgc tcatctttta tttcccggga ggggaaatgt cccgcgtatc
    20041 gcaggcgagg aacctgggta aatatttcct gctcatcgat aatatgctgg tcgtgctggg
    20101 gttctttgtt gtcttcccgc tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc
    20161 cgtcatggtc ggtattgctc tcggtctacg ccagtttatt cagcaaggtc tgggtatttt
    20221 cggcggcgca attgccgacc gctttggtgc caaaccgatg attgttaccg gtatgctgat
    20281 gcgcgccgcc ggattcgcca caatgggtat cgcccacgaa ccgtggctat tgtggttttc
    20341 atgcctgctc tcgggactcg gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt
    20401 gaaactaatc cgtccacaac aacgtggtcg ttttttctcg ctgttgatga tgcaggacag
    20461 tgccggtgcg gtcattggcg cattgttggg gagctggctg ttgcaatacg actttcgcct
    20521 ggtctgcgcc acaggggccg ttttgtttgt gctgtgtgcg gcgttcaatg cgtggttgtt
    20581 accggcatgg aaactctcca ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat
    20641 gcgtgacaag cgttttgtca cctatgtcct gacgctggcg ggttactaca tgctggctgt
    20701 acaagtgatg ctgatgctgc caattatggt caacgacgtg gctggcgcgc cctctgccgt
    20761 taaatggatg tatgccattg aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg
    20821 ctggagtgaa aagcattttc gtctggaaca ccggttgatg gctgggctgt tgataatgtc
    20881 attaagcatg atgccggtgg gcatggtcag cggcctgcaa caacttttca ccctgatttg
    20941 tctgttttat atcgggtcga tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct
    21001 ggcggacgca agagctcgcg gcagctatat ggggtttagc cgtctgggtc tggcgattgg
    21061 cggcgctatt ggttatatcg gtggtggctg gctgtttgac ctgggcaaat cggcgcacca
    21121 gccagagctt ccgtggatga tgctgggaat tattggcatc ttcactttcc ttgcgctggg
    21181 ttggcagttt agtcagaaac gcgccgcgcg tcgtttgctg gaacgcgacg cctgatttga
    21241 tactcccccc tgactcctcc atactgaaag tgaactgaca gccaaatgga ggagtcacgt
    21301 gaagctatac atttacgatc actgccctta ctgcctcaaa gcccgcatga ttttcggcct
    21361 gaaaaatatc cccgtcgaat tacatgttct gctcaacgac gacgcagaaa cacccacccg
    21421 gatggtcggt caaaaacagg ttcccattct gcaaaaagat gacagccgct atatgccaga
    21481 aagcatggat atcgttcact atgtcgataa actcgacggc aaaccgttac tgaccggcaa
    21541 acgttcccct gccattgaag agtggctgcg caaggtcaat ggctacgcca acaaactgct
    21601 gttgccgcgt tttgccaaat cggcatttga tgagttttct actcccgccg cgcgcaaata
    21661 tttcgtcgac aagaaagagg ccagcgcggg taattttgcc gacctgctgg cccactctga
    21721 cggtctgatt aagaatatca gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc
    21781 gaacgccgtg aatggagaac tttcggaaga tgatattcag ctatttccgc tactgcgtaa
    21841 tctgacgctg gtagccggaa tcaactggcc aagccgcgtt gctgattacc gcgataatat
    21901 ggcgaaacag acacaaatca atttgttatc atcaatggcg atttaatcct gacccgccgc
    21961 ggcagcctgt attcgcgggt tgccgccgtt ttcagttttt tctgctggcg agtttatcgc
    22021 tggtgacaca tgctgcgttg tgtcaggata ttgacgcaga tttcatgtcg aggactacca
    22081 tgaacaagtt tttatttgcc gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc
    22141 aactcaccca atacaccatc accgaacaag aaattaacca gtcgcttgcg aaacataata
    22201 atttctcaaa agatatcggt ttacccggcg tggctgacgc ccatattgtt ctgacaaact
    22261 tgaccagcca aattggtcgc gaagagccga ataaggttac cctaaccgga gacgccaatc
    22321 tggacatgaa ctccctgttc ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc
    22381 tgcccgtgtt tgataaagaa aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg
    22441 cgacggtaca accggaaaaa atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc
    22501 aggcattacg caattacttt aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc
    22561 agggcgaagc aatggcgaaa aaactggcga aaggcattga agtgaagcca ggcgaaattg
    22621 tcattccgtt tacggattaa tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag
    22681 gaaaacgttt ccgcttatcc tttgtgtccg gcaaaaacat cccttcagcc ggagcataga
    22741 gattaatgac tgcaccatcc caggtattaa agatccgccg cccagacgac tggcaccttc
    22801 acctccgcga tggcgacatg ttaaaaactg tcgtgccgta taccagcgaa atttatggac
    22861 gggctattgt aatgcccaat ctggctccgc ccgtgaccac tgttgaggct gccgtggcgt
    22921 atcgccagcg tattcttgat gccgtacctg ccgggcacaa tttcacccca ttgatgacct
    22981 gttatttaac agattcgctg gatcctaatg agctggagcg cggatttaac gaaggcgtgt
    23041 tcaccgctgc aaaactttat ccggcaaacg caaccactaa ctccagccac ggcgtgacgt
    23101 caattgacgc aatcatgccg gtacttgagc gcatggaaaa aatcggtatg ccgctactgg
    23161 tgcatggtga agtgacacat gcagatatcg acatttttga tcgtgaagcg cactttatag
    23221 aaagcgtgat ggaacctctg cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca
    23281 tcaccaccaa agatgctgcc gactatgtcc gtgacggaaa tgaacggctg gctgccacca
    23341 tcactccgca gcatctgatg tttaaccgca accatatgct ggttggtggc gtgcgtccgc
    23401 acctgtattg tttacccatc ctcaaacgca atattcacca acaggcattg cgtgaactgg
    23461 tcgccagcgg ttttaatcga gtattcctcg gtacggattc tgcgccacat gcacgtcatc
    23521 gcaaagagag cagttgcggc tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt
    23581 acgctaccgt ctttgaagag atgaatgctc tgcagcactt tgaagcattc tgttctgtaa
    23641 acggcccgca gttctatggc ttgccggtca acgacacatt catcgaactg gtacgtgaag
    23701 agcaacaggt tgctgaaagc atcgcactga ctgatgacac cctggtgcca ttcctcgccg
    23761 gggaaacggt acgctggtcc gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt
    23821 tattttacct gtataaataa ccagtatatt caacaggggg ctattatgcg aattgaagtc
    23881 accatagcga aaacttctcc attgccagct ggggctattg acgccctggc tggcgaactt
    23941 tcccgccgta ttcagtatgc gtttcctgat aatgaaggcc acgtatcggt acgttatgcc
    24001 gcagcgaata atttatcggt tattggcgta acaaaagaag ataaacagcg cattagcgaa
    24061 attctccagg aaacgtggga aagcgccgat gactggtttg tcagcgaata atatgcagtg
    24121 atttttgttt ttgccgggtc gccccggctt ttttatttta cttacaaatt ttgagtacaa
    24181 aatttctaca cttcctcaaa ataattgcac aatatgctaa aaaaatatga atgacatgct
    24241 gttttattgt tcatataaat cttaaaagca gaaatttatc tgttcccccc tttaaaaatt
    24301 cggtgaatac ccttacttat tggtatactg aagttgcttc agataaaaac gcattgaacc
    24361 tcgaataacg ttgtctagta acacgaatta gggggccatg atggaaaaaa ataatgaagt
    24421 cattcagact catccgctcg tagggtggga catcagcacc gttgatagct atgatgcgct
    24481 gatgttgcgt ttgcactacc agaccccaaa taagtccgag caggaaggga ctgaagttgg
    24541 tcagacgctc tggttaacca ctgatgttgc cagacaattt atttcgatat tagaagcagg
    24601 aatcgccaaa attgaatccg gtgatttccc ggtaaacgag tatcggcgtc attaacatga
    24661 catgaccatc cacccaaaag gcacctcacc ggtgccttta ccatttctgg gttgtatatc
    24721 tgcattcagt taattactct gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga
    24781 tctcatcatt attggcagcg gttccgtagg cgctgccgcc gggtattatg caacccgcgc
    24841 cggtttaaac gtgctaatga ccgacgccca tatgccaccg catcaacacg gcagccacca
    24901 cggcgatacg cgattaattc gccatgctta tggtgaaggc gaaaagtatg tcccgttggt
    24961 cctccgcgcg caaacgctgt gggatgaact ttcccgccac aacgaagatg atcccatttt
    25021 tgtacgctct ggtgtcatta acctcggccc ggctgactcc gcatttctcg ccaacgtcgc
    25081 ccacagcgcc gaacagtggc aactcaacgt tgaaaagctc gatgcgcaag ggattatggc
    25141 ccgctggcca gaaatacgcg tcccggacaa ctacatcggc ttatttgaga ctgattccgg
    25201 ttttttgcgc agcgaactgg cgattaaaac ctggatccaa ctggcgaagg aagcgggctg
    25261 tgcgcaactg ttcaactgcc cggtcaccgc aattcgtcat gacgatgatg gcgtaactat
    25321 tgaaaccgtt gacggtgagt atcaggcgaa aaaagcgatt gtctgcacgg gaacatgggt
    25381 aaaagacctg ctcccggagc tgcctgtcca gcctgtacgt aaagtatttg cctggtatca
    25441 ggccgatggc cgctatagcg tgaagaataa attcccggcg tttaccggtg aactgcccaa
    25501 tggcgatcaa tattatggtt ttccggcaga aaacgacgcg ttgaagattg gcaaacataa
    25561 cggaggccag gttatccatt cagcggatga acgtgttccg tttgcggaag tggtcagcga
    25621 tggttcggaa gccttcccgt tcttgcgcaa tgtattgccg ggtatcggtt gctgcctgta
    25681 cggcgctgcc tgcacctatg ataattcgcc tgacgaagat tttattatcg ataccctacc
    25741 cggccacgat aatacactgc tcattaccgg cctgagtggg cacggtttta aatttgcgtc
    25801 agttttaggg gaaatagctg ccgattttgc gcaagacaaa aaaagcgatt ttgatttgac
    25861 gccattcagg ctttcccgct tccaataatc atcattacgg cctccagaat gaggccgttt
    25921 ttacaggagt atttaatgcg tcggctgttg cactatctca tcaataatat tcgcgagcat
    25981 ctgatgcttt atctttttct ctggggattg ttggcaatta tggatctgat ctatgtgttt
    26041 tatttttagt tttccgcact gacataaact gacaaatatt taatctccct ttcatttagc
    26101 gtgaaaaatt gaatgaaatt attcctgctt ctacagcact caccagacat ctctgatgct
    26161 gaacccacta aacttatgaa gattctttca ttattaattc aaaattattg aaataaaaat
    26221 aaatatcatt caatttgtta tttttgtgtt gcaattcatt ctccgacacg ccattttctt
    26281 tctctcgcaa attcgtattg gatgcactta tgtcattcac aaatacccct gaacgctatg
    26341 gtgttatatc tgcggccttc cattggctaa gcgcaattat tgtctacggc atgtttactt
    26401 taggtctgtg gatggtcacg ctcagttatt acgatggctg gtatcacaaa gcacccgaac
    26461 tgcataaaag catcggtatt ttgttaatga tggggctggt tattcgcgtt ctgtggcgtg
    26521 tcatatctcc accgccgggt ccgctgccga gttattcgcc catgactcgt cttgccgcaa
    26581 aggctggaca tctcgcattg tacctattgc tgttcgccat cggcatcagc ggctatctga
    26641 tctcaactgc cgatggtaaa ccgatcagcg tttttggctg gtttgacgtc cccgcgaccc
    26701 ttgccgacgc tggcgcacag gcagactttg ccggtgccct gcatttttgg ctcgcgtgga
    26761 gcgtcgtagt actgtccgtt atgcacggat ttatggccct gaagcatcat ttcatcgata
    26821 aagacgacac tctgaagcgc atgctgggaa agtcgtcatc tgactatgga gtataaaaaa
    26881 tgaaaaaaag cctgcttggt ttaaccttcg cgtccctgat gttctctgcc ggttcagcgg
    26941 ttgccgccga ttacaaaatt gacaaagaag gtcagcacgc ctttgttaat ttccgcatcc
    27001 agcaccttgg ctatagctgg ttatacggca cctttaaaga tttcgacggt acttttacct
    27061 ttgacgaaaa aaatccggct gccgataaag tgaatgtgac aattaacacc accagcgtcg
    27121 atactaatca cgccgaacgc gataaacatc ttcgcagtgc agatttcctc aataccgcaa
    27181 aatatccaca ggcaacattc acctccacca gcgtgaagaa agacggtgac gaactggata
    27241 ttaccggcga tctgacgctg aatggcgtaa ccaaacccgt cacgctggaa gcgaaattaa
    27301 ttggtcaggg caacgaccca tggggtggta aacgtgcagg cttcgaggcc gaaggcaaaa
    27361 ttaagctcaa ggacttcaat atcaagacag atttaggtcc agcttctcag gaagtggatc
    27421 tgattatttc agtggaaggc gtacagcaga agtaagcgtg aaatgttgcc ggatggcacg
    27481 catccggcaa tgatatttat tctgttggat caggaatgcc cagtgttgta ttcagacgtc
    27541 cacgagactt attaaagatc ttattgccat tttcacgtcc tgcccgacgg cgtcgctgtt
    27601 cttctggcgg taacgcgctt tcttcgcagc aaatctcact acaacaacct ttgtattttt
    27661 ccgcgcatac tggacactga ataaacagca ggtggcagcc atcattttta cagttggtat
    27721 ggctgtcgca cggcgcgccg cactggtggc aatgcgcgat aatctcatcc gatatacgtt
    27781 cgcccatccg ctcgtcaaaa acaaaatttt tgccaataaa acgcaccggc aagccctgct
    27841 cgcgcgcctt acgggcgtat tcaataattc cgccctcgat atgccagact ttgttgaatc
    27901 cgttatgttt catccaagca ctggcctttt cacaacgaat gccgccggtg cagtacatga
    27961 cgattttttt atctttatgt gcctgcatca tctcgactgc ttttggcagc tgctcacgga
    28021 aggtatctgc cggaatttcg agcgcgtttt caaagtgccc cacttcatac tca
//
LOCUS       DAJTNN010000033        28011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTNN010000033 DAJTNN010000000
VERSION     DAJTNN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.033"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..88)
                     /locus_tag="OO150_001305"
     CDS             complement(<1..88)
                     /locus_tag="OO150_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5515508.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILT"
     gene            complement(88..702)
                     /locus_tag="OO150_001306"
     CDS             complement(88..702)
                     /locus_tag="OO150_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5515509.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(692..2683)
                     /gene="cyoB"
                     /locus_tag="OO150_001307"
     CDS             complement(692..2683)
                     /gene="cyoB"
                     /locus_tag="OO150_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR5515510.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWANSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(2705..3652)
                     /gene="cyoA"
                     /locus_tag="OO150_001308"
     CDS             complement(2705..3652)
                     /gene="cyoA"
                     /locus_tag="OO150_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR5515511.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(4113..5588)
                     /gene="ampG"
                     /locus_tag="OO150_001309"
     CDS             complement(4113..5588)
                     /gene="ampG"
                     /locus_tag="OO150_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5515512.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(5632..6210)
                     /locus_tag="OO150_001310"
     CDS             complement(5632..6210)
                     /locus_tag="OO150_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5515513.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLAASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            6515..6832
                     /gene="bolA"
                     /locus_tag="OO150_001311"
     CDS             6515..6832
                     /gene="bolA"
                     /locus_tag="OO150_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5515514.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            7176..8474
                     /gene="tig"
                     /locus_tag="OO150_001312"
     CDS             7176..8474
                     /gene="tig"
                     /locus_tag="OO150_001312"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5515515.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDSAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            8719..9342
                     /gene="clpP"
                     /locus_tag="OO150_001313"
     CDS             8719..9342
                     /gene="clpP"
                     /locus_tag="OO150_001313"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5515516.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            9468..10742
                     /gene="clpX"
                     /locus_tag="OO150_001314"
     CDS             9468..10742
                     /gene="clpX"
                     /locus_tag="OO150_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5515517.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            10930..13284
                     /gene="lon"
                     /locus_tag="OO150_001315"
     CDS             10930..13284
                     /gene="lon"
                     /locus_tag="OO150_001315"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5515518.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            13493..13765
                     /gene="hupB"
                     /locus_tag="OO150_001316"
     CDS             13493..13765
                     /gene="hupB"
                     /locus_tag="OO150_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5515519.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            13957..15828
                     /gene="ppiD"
                     /locus_tag="OO150_001317"
     CDS             13957..15828
                     /gene="ppiD"
                     /locus_tag="OO150_001317"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5515520.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            15979..16350
                     /locus_tag="OO150_001318"
     CDS             15979..16350
                     /locus_tag="OO150_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5515521.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            16444..16842
                     /gene="fadM"
                     /locus_tag="OO150_001319"
     CDS             16444..16842
                     /gene="fadM"
                     /locus_tag="OO150_001319"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5515522.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(16894..17589)
                     /gene="queC"
                     /locus_tag="OO150_001320"
     CDS             complement(16894..17589)
                     /gene="queC"
                     /locus_tag="OO150_001320"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5515523.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(17654..19354)
                     /locus_tag="OO150_001321"
     CDS             complement(17654..19354)
                     /locus_tag="OO150_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR5515524.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLALG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            19454..20236
                     /gene="cof"
                     /locus_tag="OO150_001322"
                     /pseudo
     CDS             19454..20236
                     /gene="cof"
                     /locus_tag="OO150_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
     gene            20425..20883
                     /gene="decR"
                     /locus_tag="OO150_001323"
     CDS             20425..20883
                     /gene="decR"
                     /locus_tag="OO150_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5515525.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            20913..22685
                     /locus_tag="OO150_001324"
     CDS             20913..22685
                     /locus_tag="OO150_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5515526.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASENIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            22678..24459
                     /locus_tag="OO150_001325"
     CDS             22678..24459
                     /locus_tag="OO150_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5515527.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            24640..24978
                     /gene="glnK"
                     /locus_tag="OO150_001326"
     CDS             24640..24978
                     /gene="glnK"
                     /locus_tag="OO150_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5515528.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            25008..26294
                     /gene="amtB"
                     /locus_tag="OO150_001327"
     CDS             25008..26294
                     /gene="amtB"
                     /locus_tag="OO150_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5515529.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(26343..27203)
                     /gene="tesB"
                     /locus_tag="OO150_001328"
     CDS             complement(26343..27203)
                     /gene="tesB"
                     /locus_tag="OO150_001328"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5515530.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            27421..27993
                     /locus_tag="OO150_001329"
     CDS             27421..27993
                     /locus_tag="OO150_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5515531.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
BASE COUNT         7045 a         6822 c         7634 g         6510 t
ORIGIN      
        1 ccgtcagaat gatcgacagg ataaagcctg tcatgtaggt ttttacgctg ccatgggacg
       61 cgccgctgtg atcggtagaa tgactcatta catcgccccc atcagataaa caacagtgaa
      121 cacacagatc caaaccacat ccaggaagtg ccagaacagg ctcaggcaca tgatgcgggt
      181 acggttagtg ctggtcaggc cgcgacgagc gatttgtacc atcagcaccg ccatccagat
      241 aagaccggaa gtgacgtgca gaccgtgcgt gccgaccagc gcaaagaacg ctgacaggaa
      301 gccgctgcga tccggaccca tgccgttaac aatcaggtga tggaattcgt agatttccat
      361 cccgataaat ccggcaccaa acaaacaggt caacgccagc caggagataa cctggctttt
      421 gttgtttttg tacatggcga tagccgccat gccgtaggtg atggagctga acaacagcaa
      481 gaaagtttca accagaacga acggcagttc gaaaatgtcc ttacctgtcg ggccgcctgc
      541 ggtgccgttc accagaacgg cataggtagc aaacaagatt gagaacagaa tgcagtcgct
      601 catcaggtag atccaaaatc cgaagatttt ggttccgcct gcatcgtggt gcccgtgttc
      661 gtgcgcgtgg gcagtcgcgt gcgtcaaagt atcagttgcc atttttcagc cctgccttag
      721 taatctcatc gaaatgctgg ttttccagtt tttcgatttc tgccaccggc acgtagtaat
      781 ccacgtcctc gtcgaagctt ttcacgatcc aggtgatgat catgcctgcg aagccaacaa
      841 tcgccagcca ccagatatgc cagatcatgg cgaaaccgaa gatggtggag aaagctgcaa
      901 tgacgatacc tgcaccgctg tttttcggca tatgaatttc ttcatagtgg tcaggctttt
      961 tatacgcttc gcctttctct ttcatttccc agaatgcatc acgctcgtga acgtgcggca
     1021 caacggcaaa gttatagaac ggaggcgggg aagagtttgc ccactccagc gtacggccac
     1081 cccacgggtc gccagtcagg tcacggttct ggtcgcggtc gcgaatagaa acgtacatct
     1141 gaataacgag gcagagaata cccagcgcaa tcagtactgc accgctagct gcaatcatca
     1201 gcatggtgtg gaactgcggg tcaatctgct ggctcaaacg acgggtcatg cccatgaagc
     1261 ccagcgcata cagcggcata aaggcaacga agaagccgat gatccagaac cagaacgcgc
     1321 gtttacccca ggtttcattc agtttgaaac cgaacgcttt aggccaccag taggtcatcc
     1381 ctgcgaagca gccgaagacc acgccgccga tgataacgtt atggaagtgc gcaatcagga
     1441 acaggctgtt atgcagaacg aagtccgcgc ccggtactgc cagcagcacg ccagtcatcc
     1501 cgcccaccga gaaagtgacg ataaaaccga tggtccacag catcgcagaa tggaacacga
     1561 tacggccctg atacatggtg aacagccagt tgaagatctt caccccggtc gggatggcga
     1621 taatcattgt ggtgatacca aagaaggcgt ttacgttcgc gcccgcaccc atcgtaaaga
     1681 agtggtgcag ccaaacgatg aacgacagca cggtgataca gacggttgcc cataccagcg
     1741 aggtataacc aaacagacgt ttacgcgaga aggttgccgc aatttcggag aacacaccga
     1801 aaacaggcag gatcaggatg taaacttccg ggtggcccca ggcccaaatc aggttgatgt
     1861 acatcatcat gttgccaccc atatcgttgg taaagaaatg ggtgcccaga tagcgatcca
     1921 gggtcaacaa cgcgacggta accgtcagaa ttgggaagga agcaataatc agtacgttcg
     1981 cgcacagtga tgcccaggta aatactggca tcttgaacat ggtcatgccc ggtgcgcgca
     2041 tcttcagaat ggtaacgaag aagttgatac cggtaagcgt cgtacctata ccggatagct
     2101 ggagactcca tatccagtaa tcaacaccga ctcccggact gtactctatt cccgatagcg
     2161 gtggataggc cagccagccg gtctgcgcaa attcgcccac gccgagagaa acgttaacca
     2221 gaatcacacc aacaacagta aaccagaagc ttaagttgtt gaggaacggg aacgcaacgt
     2281 cacgcgcgcc gatctgcagc ggaaccacca ggttcatcag accgataacg aaaggcatcg
     2341 ccacgaagaa gatcataatc acgccgtgcg cggtgaagat ctgatcgtag tggtgaggtg
     2401 gcaggaagcc cgcttcgccc gccgaggcaa gagcctgctg gctacgcatc ataatggcgt
     2461 cagcaaaacc acgcagcaac atcacaatcg ccacgatgat atacatgata ccgaggcgtt
     2521 tatggtcgac ggaggtcagc cactctttcc acaggtaggt ccacttaccg aagtaagtga
     2581 tcaggccaac gagcgccaga cctcccaaaa taatgccagc gatcgtaacc atgacgatag
     2641 gttcatggaa cgggactgca tcaagtgata attttccgaa catctttatt cttcctcaac
     2701 ccctttaatg ggcggattcc gcgtggctca tgtccatgcc ttccatacct tcgtgtgcgc
     2761 tgtgctcacc ttctggctgg gtcatgtcca tgctcttacc gtgagccata aacttgttaa
     2821 tcacatcagc aaacaagtct ggtttcacgt tggagaaata ttccacctgg ttgtattcgc
     2881 taggcgcggc cagtttttcg aacgcagcca tgtcagacat ggtgttcggc gactgcttcg
     2941 cttttgcgac ccactggtcg aattcggcgc gatccggtgt tgcaatagct ttgaacttca
     3001 tgcctgagaa gcccgggccg ctgtagctgg cggagatacc gtcataagta ccgggttcgt
     3061 tggcgatcag atgcaggcga gtctgcatac cagccatggc ataaatctgg ctacccagac
     3121 gcggaatgaa gaaggagttc atcacggagt tggaggtcac tttgaagtac accggagtgt
     3181 tcgccgggaa agcgatttca ttcacggtag caatgccctg ttccgggtag atgaagaacc
     3241 atttccagtc catggaaacc acttcgatgg taatgggctt ctcgtcgtgt gccagcggct
     3301 tgctaggctc aagagcgtga gtggttttcc aggtcagtac cgcaaggaag atgatgatta
     3361 agataggtac cgtccagacc acagcttcca ctttattgga gtgtgaccag ttcgggctgt
     3421 acttagcatc tttattgctc gcacgatact tccaggcgaa accaacagcc atcaagattg
     3481 cgggaataac gacaatcaac atcaggccaa atgccgtcag tatcagtgaa cgttgctcca
     3541 gaccaatctg tcctttggga tctaacagcg cagaattaca gccactgagc aatacagtgc
     3601 ctgcaaataa tgacaaccat cccaaacttt tattgtattt cctgagtctc atttaacgac
     3661 ctcaattcca cgggatctgg tggcgtttaa agtgtgtggg cattttatgg gaaggttaca
     3721 ttactgtaaa catgattaaa ttcctgttac ttggatttga ttgtcaggtc acatatgttg
     3781 caaaacatat caagttgatt ttaagaagct gttgcaaaag ggcgttataa cgaaagggga
     3841 gtgcagaagg agtttggatg ggcaattggt ataaccaatc tggaataaaa taattgttta
     3901 acaaataatt atcattatgg tgacaattgg gtggggggaa aataaagact cgttagctga
     3961 atcgtgtaag aaaaaataga attcataaat tgcgtcaata atttccaaac gctaagccgc
     4021 acaaaagaac aaatattatt aagcgatact gatgtttatt tagccgtaat aattacggcg
     4081 agtgattact acagctaaat aatatttaca gattacgtca gatgcgtttt tcgtagagcc
     4141 agataatcca gcaaaccacc aagcacgacg ccagaaaggg cgactaaaac cccgacttcc
     4201 agcagagcag gcaggaaaga gaagtgcgtc aaatccagcg cgtccatcgt caacagcagt
     4261 aaccacacgg ccaacaggct gacgcccgcc gccagtgtcc acatggcaaa ggcataacct
     4321 gccggatatt cggtacggga gataaagttg tcatttactc gtgtatattc aagcgtctgg
     4381 cggcaaacca gcagcaaaat aagccccggt acggcagcgg cgacggagaa tagatagaat
     4441 gtcgaccagc cgtgtgcttc aacaaaccaa cccgccacgg ggccgacata aactcgtcct
     4501 acagcggaaa gcgctgagag cagggcaaat tgagtagcgg aaaatgactt attacacagc
     4561 gtcattaaca gcgcgacaaa ggctgatgtg cccatcccgc cacagaggtt ttcgaaaaag
     4621 acggctgcgc ccatgctgta gagatgctta tcagtaatcg acagcagcca gtaaccagcg
     4681 ttagacgcac cttgtaaaat gccgaaaatc agcagcgccc ggaacagcga caggcgctgc
     4741 atcaaaatcc caccgtacaa tgcgcccaca atggtcgcca gtaagccaag cgttttgtta
     4801 accacgccta cttcacccgc atcaaacccg acgccgcgaa tcaaaaacgt ggttgtcagg
     4861 ctcatggcga atgcgtcgcc cagcttatac agcacgataa gaagcaaaat aagccaggca
     4921 ttattgcgac caaagaaatc tcgcagaggt gcaacaaccg cttgttccag cgtttttggc
     4981 acaggaatgg tgtcggtcgg ttctggtgca agcaacgtcg cgataataca agggatcaac
     5041 agcgccgcca tcaaccagta catgccctgc cagcccagcc atttatctgc cagccacagg
     5101 gctaagccgc cggaaaccag catccctaaa cggtaaccca gcacgctgat tgccgcgccc
     5161 gcaccacgtt cttctgccgg gagcacatcg gttttccacg catcgaagac aatatcctgg
     5221 gaggccgagc aaaaagcgat caccaccgcc agtgccgcca tccagcggag ttgggtgcca
     5281 ggttcgagaa aacccatcgc cgcaatggcg actaataaca ggatttgcgt ggcgagcagc
     5341 caaccgcgcc gccgcccaaa aaatgggggc gtgtagcggt ccatcagcgg tgaccagagg
     5401 aatttaaaaa cgtaagcctg gcctaccaga gagaagaaac caatggtttt gagatcaata
     5461 ttctcgaccg tcatccaagc ctgtaaggta ccggatgtca gggcgagcgg tagcccggaa
     5521 gcaaaaccca ggatcagcaa tatggctgaa cgcggctgtt gaaaaatacg taaatattga
     5581 ctggacatgg gcgtctacag gcccggtttg caccgggcca gagggcagaa attaacgcgc
     5641 gttctgcttg atgaattcgt gaatgctggt gtcctgagac atatcagcga tggtatcggt
     5701 cagcacactg ttaaccgcat cggcgatatt tttgttggag gcctggaacg caccttcaac
     5761 gttgtagctg gcacgatagt ttttggttat tttgttgcca ttctgcgcgg tagcgatgat
     5821 ggcgatatcc gctttggtag cgatgttgta gcgcacgttg ccctgggaca cgtcagcata
     5881 cagttggcta acgatgattt gcagattaac cgggccattc ggaccaacca tgtaaccacg
     5941 cgcggtcatc tgtttttcca gcacttcttg cagcaggaaa cgcagatcgc gggaggcggc
     6001 cagggtaacg atttgattat cgcgggtgac ttttgccagc gcctgatcgg tacgctgatc
     6061 ggcaccatta atgcttacgg tgacgcccat caggcttgga tcctgctgtg gcagtgtaat
     6121 cgtcggggaa acttcaatag ttgttggcgg ttttgcgcat cctgcaagca taaacagagc
     6181 aactaacggg aagaggattt ttttgaacat gttcgggctc tcagagactc ttaagcgtgt
     6241 ttggtaaaaa ttcccgccat cataacattg ccaacggcga ggggaagtgg gtaaggcatg
     6301 taaattcatc atgttgacga aataatcacc cctggtaaaa gaaacgctga tgcgaggcct
     6361 gtgtttcaat ctttaaatca gtaaacttca tacgcttgac ggaaaaacca ggacgaaacc
     6421 taaatatttg ttgttaagct gcaatggaaa cggtaaaagc ggctagtatt taaagggatg
     6481 gatgacatct cagcgttgtc ggaggagata tttcatgatg atacgtgagc ggatagaaga
     6541 aaaattaagg gcggcgttcc aacccgtatt cctcgaagta gtggatgaaa gctatcgtca
     6601 caatgtccca gccggctctg aaagccattt taaagttgtg ctggtcagcg atcgttttac
     6661 gggtgaacgt tttctgaatc gtcatcgaat gatttacagt actttagcgg aggaactctc
     6721 tactaccgtt catgcgctgg ctctgcatac ttacactatt aaggagtggg aagggttgca
     6781 ggacaccgtc tttgcctctc ctccctgtcg tggagcagga agcatcgcgt aaaaacgcat
     6841 ttgcaactgt cggcgctttt tcagtatgtt gctaaagatt ttatgaaaaa cggcctgcgg
     6901 gccgttttgt tttgtctgga ttttgcgctt tttgcccagc attcagacga aaattgcccg
     6961 ggaattgtga aaaaatacgc gacagcgcgc aataaccgtt ctcgactcat aaaagtgatg
     7021 ccgctataat gccgcgtcct atttgaatgc tttcgggatg attctggtaa cagggaatgt
     7081 gattgattat aagaacatcc cggttccgcg aagccaacaa cctgtgcttg cggggtaaga
     7141 gttgaccgag cactgtgatt ttttgaggta acaagatgca agtttcagtt gaaaccactc
     7201 aaggccttgg ccgccgtgta acgattacta tcgctgctga cagcatcgag accgctgtta
     7261 aaagcgagct ggtcaacgtt gcgaaaaaag tacgtattga cggcttccgc aaaggcaaag
     7321 tgccaatgaa tatcgttgct cagcgttatg gcgcgtctgt acgccaggac gttctgggtg
     7381 acctgatgag ccgtaacttc attgacgcca tcattaaaga aaaaatcaat ccggctggcg
     7441 caccgactta tgttccgggc gaatacaagc tgggtgaaga cttcacttac tctgtagagt
     7501 ttgaagttta tccggaagtt gaactgcagg gtctggaagc gatcgaagtt gaaaaaccga
     7561 tcgttgaagt gaccgacgct gacgttgacg gcatgctgga tactctgcgt aaacagcagg
     7621 cgacctggaa agaaaaagac agcgctgttg aagcagaaga ccgcgtaacc atcgacttca
     7681 ccggttctgt agacagcgaa gagttcgaag gcggtaaagc gtctgatttc gtactggcga
     7741 tgggccaggg tcgtatgatc ccgggctttg aagacggtat caaaggccac aaagctggcg
     7801 aagagttcac catcgacgtg accttcccgg aagaatacca cgcagaaaac ctgaaaggta
     7861 aagcagcgaa attcgctatc aacctgaaga aagttgaaga gcgtgaactg ccggaactga
     7921 ctgcagaatt catcaaacgt ttcggcgttg aagatggttc cgtagaaggt ctgcgcgctg
     7981 aagtgcgtaa aaacatggag cgcgagctga agagcgccat ccgtaaccgc gttaagtctc
     8041 aggcgatcga aggtctggta aaagctaacg acatcgacgt accggctgcg ctgatcgaca
     8101 gcgaaatcga cgttctgcgt cgccaggctg cacagcgttt cggtggcaac gaaaaacaag
     8161 ctctggaact gccgcgcgaa ctgttcgaag aacaggctaa acgccgcgta gttgttggcc
     8221 tgctgctggg cgaagttatc cgcaccaacg agctgaaagc tgacgaagag cgcgtgaaag
     8281 gcctgatcga agagatggct tctgcgtacg aagatccgaa agaagttatc gagttctaca
     8341 gcaaaaacaa agaactgatg gacaacatgc gcaatgttgc tctggaagaa caggctgttg
     8401 aagctgtact ggcgaaagcg aaagtgactg aaaaagaaac cactttcaac gagctgatga
     8461 accagcaggc gtaatttacg cagcataacg cgctaaattc gcacaaaggc ccgtcaccgc
     8521 caggtggtgg gctttttttg tcatgaattt tgcatggaac cgtgcgaaaa gcctctttcg
     8581 gtgttagcgt aacaacaaaa gattgttatg cttgaaatat ggtgatgccg tacccataac
     8641 acagggacta gctgataatc cgtccataag gttacaatcg gtacagcagg ttttttcaat
     8701 tttatccagg agacggaaat gtcatacagc ggcgaacgag ataactttgc accccatatg
     8761 gcgctggtgc cgatggtcat tgaacagacc tcacgcggtg agcgctcttt tgatatctat
     8821 tctcgtctac ttaaggaacg cgtcattttt ctgactggcc aggttgaaga ccacatggct
     8881 aacctgattg tggcgcagat gctgttcctg gaagcggaaa acccagaaaa agatatctat
     8941 ctgtacatta actccccagg cggggtgatc actgccggga tgtctatcta tgacaccatg
     9001 cagtttatca agcctgatgt cagcaccatc tgtatgggcc aggcggcctc gatgggcgct
     9061 ttcttgctga ccgcaggggc aaaaggtaaa cgtttttgcc tgccgaattc gcgcgtgatg
     9121 attcaccaac cgttgggcgg ctaccagggc caggcgaccg atatcgaaat tcatgcccgt
     9181 gaaattctga aagttaaagg gcgcatgaat gaacttatgg cgcttcatac gggtcaatca
     9241 ttagaacaga ttgaacgtga taccgagcgc gatcgcttcc tttccgcccc tgaagcggtg
     9301 gaatacggtc tggtcgattc gattctgacc catcgtaatt gatgccagag gcgcaactgt
     9361 gccgctatac ttatccaggg cggcacaacg ctgtaagcgg cttgcgcctg agaatggcat
     9421 ttgcgtcgtc gtgtgcggca caaagaacaa agaagaggtt ttgacccatg acagataaac
     9481 gcaaagatgg ctcaggcaaa ttgctgtatt gctctttttg cggcaaaagc cagcatgaag
     9541 tgcgcaagct gattgccggt ccatccgtgt atatctgcga cgaatgtgtt gatttatgta
     9601 acgacatcat tcgcgaagag attaaagaag ttgcaccgca tcgtgaacgc agtgcgctac
     9661 cgacgccgca tgaaattcgc aaccacctgg acgattacgt tatcggccag gaacaggcga
     9721 aaaaagtgct ggcggtcgcg gtatacaacc attacaaacg tctgcgcaac ggcgatacca
     9781 gcaatggtgt cgagttgggc aaaagtaaca ttctgctgat cggtccgacc ggttccggta
     9841 aaacgctgct ggctgaaacg ctggcgcgcc tgctggatgt tccgttcacc atggccgacg
     9901 cgactacact gaccgaagcc ggttatgtgg gtgaagacgt tgaaaacatc attcagaagc
     9961 tgttgcagaa atgcgactac gatgtccaga aagcacagcg tggtattgtc tacatcgatg
    10021 aaatcgacaa gatttctcgt aagtcggaca acccgtctat tacccgtgac gtttccggtg
    10081 aaggcgtaca gcaggcactg ttgaaactga tcgaaggtac tgttgctgcc gttccaccgc
    10141 agggcgggcg taagcatccg cagcaggaat ttttgcaggt tgatacctcc aagatcctgt
    10201 ttatttgtgg cggtgcgttt gccggtctgg ataaagtgat ttcccaccgt gtagaaaccg
    10261 gctccggcat tggttttggc gcgacggtaa aagcgaagtc cgacaaagca agcgaaggcg
    10321 agctgctggc gcaggttgaa ccggaagatc tgatcaagtt tggtcttatc cctgagttta
    10381 ttggtcgtct gccggttgtc gcaacgttga atgaactgag cgaagaagct ctgattcaga
    10441 tcctcaaaga gccgaaaaac gccctgacca agcagtatca ggcgctgttt aatctggaag
    10501 gcgtggatct ggaattccgt gacgaggcgc tggatgctat cgctaagaaa gcgatggcgc
    10561 gtaaaaccgg tgcccgtggc ctgcgttcca tcgtagaagc cgcactgctc gataccatgt
    10621 acgatctgcc gtccatggaa gatgtcgaaa aagtggttat cgacgagtcg gtaattgatg
    10681 gtcaaagcaa accgttgctg atttatggta agccggaagc gcaacaggca tctggtgaat
    10741 aattaaccat tcccatacaa ttagttaacc aaaaaggggg gattttatct cccctttaat
    10801 ttttcctcta ttctcggcgt tgaatgtggg ggaaacatcc ccatatattg acgtacatgt
    10861 taatagatgg cgtgaagcac agtcgtgtca tctgattacc tggcggaaat taaactaaga
    10921 gagagctcta tgaatcctga gcgttctgaa cgcattgaaa tccccgtatt gccgctgcgc
    10981 gatgtggtgg tttatccgca catggtcatt cccttatttg tcgggcggga aaaatctatc
    11041 cgttgtctgg aagcggcgat ggaccatgat aaaaaaatta tgctggtcgc gcagaaagaa
    11101 gcttcaacgg atgagccggg tgtaaacgat cttttcaccg tcgggaccgt ggcctctata
    11161 ttgcagatgc tgaaactgcc tgacggcacc gtcaaagtgc tggtcgaggg gttacagcgc
    11221 gcgcgtattt ctgcgctctc tgacaatggc gaacactttt ctgcgaaggc ggagtatctg
    11281 gagtcgccga ccattgatga gcgggaacag gaagtgctgg tgcgtactgc aatcagccag
    11341 ttcgaaggct acatcaagct gaacaaaaaa atcccaccag aagtgctgac gtcgctgaat
    11401 agcatcgacg atccggcgcg tctggcggat accattgctg cacatatgcc actgaaactg
    11461 gctgacaaac agtccgttct ggagatgtcc gacgttaacg aacgtctgga atatctgatg
    11521 gcaatgatgg aatcggaaat cgatctgctg caggttgaga aacgcattcg caaccgcgtt
    11581 aaaaagcaga tggagaaatc ccagcgtgag tactatctga acgagcaaat gaaagctatt
    11641 cagaaagaac tcggtgaaat ggacgacgcg ccggacgaaa acgaagccct gaagcgcaaa
    11701 atcgacgcgg cgaagatgcc gaaagaggca aaagagaaag cggaagcaga gttgcagaag
    11761 ctgaaaatga tgtctccgat gtcggcagaa gcgaccgtag tgcgtggtta tatcgactgg
    11821 atggtacagg taccgtggaa tgcgcgcagc aaggtcaaaa aagacctgcg tcaggcgcag
    11881 gaaatccttg ataccgacca ttatggtctg gagcgcgtga aagatcgcat ccttgagtac
    11941 cttgcggttc aaagccgtgt caacaaaatc aagggaccga tcctctgcct ggtagggccg
    12001 ccgggggtag gtaaaacctc tcttggtcag tccattgcca aagccaccgg gcgtaaatat
    12061 gtccgtatgg cgctgggcgg cgtgcgtgat gaagcggaaa tccgtggtca ccgccgtact
    12121 tacatcggtt ctatgccggg taaactgatc cagaaaatgg cgaaagtggg cgtgaaaaac
    12181 ccgctgttcc tgctcgatga gatcgacaaa atgtcttctg acatgcgagg cgatccggcc
    12241 tctgcactgc ttgaagtgct ggatccagag cagaacgtag cgttcagcga ccactacttg
    12301 gaagtggatt acgatctcag cgacgtgatg tttgtcgcga cgtcgaactc catgaacatt
    12361 ccggcaccgc tgctggatcg tatggaagtg attcgcctct ccggttatac cgaagatgaa
    12421 aaactgaaca tcgccaaacg tcacctgctg ccgaagcaga ttgaacgtaa tgcactgaaa
    12481 aaaggtgagc tgaccgtcga cgatagcgcc attatcggca ttattcgtta ctacacccgt
    12541 gaggcgggcg tgcgtggtct ggagcgtgaa atctccaaac tgtgccgcaa agcggttaag
    12601 cagttactgc tcgataagtc attaaaacat atcgaaatta acggcgataa cctgcatgac
    12661 tacctcggtg ttcagcgttt cgactatggt cgcgctgata acgaaaactg tgtcggtcag
    12721 gtaaccggtc tggcgtggac ggaagtgggc ggtgacttgc tgaccattga aaccgcatgt
    12781 gttccgggta aaggcaaact gacctatacc ggttcgctcg gcgaagtgat gcaggagtct
    12841 attcaggcgg cgttaacggt ggttcgtgcg cgtgcggaaa aactggggat caaccctgat
    12901 ttttacgaaa aacgtgacat ccacgtccac gtaccggaag gtgcgacgcc gaaagatggt
    12961 ccgagtgccg gtattgctat gtgcaccgcg ctggtttctt gcctgaccgg taacccggtt
    13021 cgtgccgatg tggcaatgac cggtgagatc actctgcgtg gtcaggtact gccgatcggt
    13081 ggtttgaaag aaaaactcct ggcagcgcat cgcggcggga ttaaaacagt gctaattccg
    13141 ttcgaaaata aacgcgatct ggaagagatt cctgacaacg taattgccga tctggacatt
    13201 catcctgtga agcgcattga ggaagttctg actctggcgc tgcaaaatga accgtctggc
    13261 atgcaggttg tgactgcaaa atagtgacct cgcgcaaaat gcactaataa aaacagggct
    13321 ggcaggctaa ttcgggcttg ccagcctttt tttgtctcgc taagttagat ggcggatcgg
    13381 gcttgccctt attaaggggt gttgtaaggg gatggctggc ctgatataac tgctgcgcgt
    13441 tcgtaccttg aaggattcaa gtgcgatata aattataaag aggaagagaa gagtgaataa
    13501 atctcaattg atcgacaaga ttgctgcagg ggctgatatc tctaaagctg cggctggccg
    13561 tgcgttagat gctattattg cttccgtaac tgaatctctg aaagaagggg atgatgtagc
    13621 actggtaggt tttggtactt ttgccgttaa agagcgtgct gcccgtactg gccgcaaccc
    13681 gcagaccggt aaagagatca ccatcgctgc tgctaaagta ccgagcttcc gtgcaggtaa
    13741 agcactgaaa gacgcggtaa actaagcgtt gtccccggtg gggatgtgac gaagttcaag
    13801 ggcgcatcta ctgatgtgcc ttttttattt gtattcggtg actttctgcg tcttgtgggc
    13861 tgacaattgc ccccgtttct tgtcacaata ggcctttgcg cgcatcgata cgttgcgtga
    13921 ggtacacagt catctacagc ggagtgttgt tacaccatga tggacagctt acgcacggct
    13981 gcaaacagtc tcgtgctcaa gattattttc ggtatcatta tcgtgtcgtt catattgacc
    14041 ggcgtgagtg gttacctgat tggcggaggc aataactacg ccgcaaaagt gaatgaccag
    14101 gaaatcagcc gtgggcagtt cgagaacgcc tttaacagcg agcgtaatcg catgcagcaa
    14161 cagctgggcg atcaatactc cgagctggca gcgaatgaag gctatatgaa aaccctgcgt
    14221 caacaggtgc tgaatcgtct gatcgacgag gcgctgttgg atcagtacgc tcgtgagctg
    14281 aaactgggta tcagtgatga gcaggttaaa caggcgattt tcgcgacccc agccttccag
    14341 gttgacggca aatttgataa cagccgctat aacggtatcc tcaaccagat ggggatgacc
    14401 gccgatcagt acgcccaggc gctgcgtaac cagctcacta cccaacagct gattaacggt
    14461 gttgccggta ccgattttat gctgaaaggt gaaaccgacg agctggcggc actggtcgcg
    14521 caacaacgcg tggtgcgtga ggcgactatc gatgttaacg cgctggcggc gaagcagcct
    14581 gtgaccgaac aggagattgc cagctactac gaacaaaaca aaaacaattt catgacgccg
    14641 gaacaattcc gcgtgagtta catcaagctg gatgccgcaa cgatgcagca accggttagc
    14701 gatgcggata tccagagcta ctacgatcag catcaggatc aattcaccca gccgcagcgt
    14761 acccgctaca gcatcatcca gaccaaaact gaagatgaag cgaaagcggt acttgatgag
    14821 ctgaataaag gcggtgattt tgctgcatta gccaaagaaa aatctgccga tattatctct
    14881 gcccgtaacg gcggcgatat gggttggtta gaagatgcca ctatccccga cgaactgaaa
    14941 aatgctggtc tgaaagaaaa aggccaactg tctggtgtca tcaaatcttc ggtcggtttc
    15001 ctgattgtac gtctggacga cattcagcca gcgaaagtga aatcgttaga cgaagtacgt
    15061 gacgacattg cggcgaaagt gaaacacgaa aaagccctcg atgcgtacta tgcgctgcag
    15121 cagaaagtga gcgatgcggc aagcaacgac accgagtctc tggccggtgc agagcaagct
    15181 gccggcgtta aagccactca gacgggttgg ttcagcaaag ataacctgcc ggaagagttg
    15241 aacttcaagc cggttgccga cgctatcttt aacggcggtc tggtaggtga aaacggcgcg
    15301 ccgggcatca actctgacat catcaccgta gacggcgacc gtgcattcgt gctgcgcatc
    15361 agcgagcaca aaccggaagc ggtgaaaccg ttggcagatg ttcaggaaca agttaaggca
    15421 ctggttcagc acaacaaagc tgaacaacag gcgaaagtgg atgctgagaa actgctggtt
    15481 gatttgaaag ccggcaaagg tgcggaagct atgcaggctg ccggtctgaa atttggcgag
    15541 ccgaaaacct taagccgttc cggtcgtgac ccgattagcc aggcggcgtt tgcactgcca
    15601 ctgccagcga aagacaaacc gagctacggt atggcgaccg atatgcaagg taatgtggtt
    15661 ctgctggcgc tggatgaagt gaaacaaggt tcaatgccgg aagatcagaa aaaagcgatg
    15721 gtgcagggta tcacccagaa caacgcacaa atcgtctttg aagctctgat gagtaacctg
    15781 cgtaaagagg cgaaaatcaa aattggcgat gcgctggaac agcaataatc ctgaagccgc
    15841 ctcgcaaaaa aatgcgttgc agctgtaaca agaaaaggtc gctttcgcgg ccttttccat
    15901 ttctgaacat tgccatttgt ttactgtttt cactgccgtt aaggtgattc cactgttaac
    15961 aaacaaggag aaaacagtat gaaacacgga attaaagcac tgctcattac cctgtccctg
    16021 gcctgtgccg gaatgtctca tagcgcgctg gcggcagctt ctgtggcgaa accgacggcg
    16081 gtagaaacca aagcggaagc tcctgcagca caaagtaaag cagcagtacc ggcgaaagcc
    16141 agtgacgaag aaggcacccg ggtcagcatt aataatgcca gcgcggaaga gctagcccgc
    16201 gcgatgaatg gcgttggcct gaagaaagcg caggcgattg tcagttatcg cgaagagtac
    16261 ggtccgttta aaacagtgga ggatctaaag caggtgccgg ggatgggcaa ttcgctggtg
    16321 gaacgtaatc tggcggtatt aaccctgtaa ttaatttgca tagtggcaat tttttgccag
    16381 actgaagagg tcataccagt tatgacctct gtacttataa caacaacgta aggttattgc
    16441 gctatgcaaa cacaaataaa agttcgtgga tatcatctcg acgtttacca gcacgtcaac
    16501 aacgcccgct accttgaatt tctcgaagaa gcccgctggg atgggttgga aaatagcgac
    16561 agttttcagt ggatgacggc ccataacatc gccttcgtcg tggtcaatat caatattaac
    16621 tatcgtcgcc cagcggtatt aagtgacctg ttaactatta ccagtcagtt gcagcaatta
    16681 aacggtaaaa gcggcatctt aagccaggtc attacactgg agccggaagg gcaggtggta
    16741 gcggatgcgc ttattacgtt tgtttgtatt gatcttaaaa cgcagaaagc attagctctg
    16801 gaaggggaat tgcgcgaaaa gctggagcag atggttaagt aaacgttttg tggtgccgga
    16861 tgctcaagcc gcatccggcg acacccggaa taattacctc aacccggttt tctgcttcat
    16921 cgctgccatc accgtcggtt tatcggccag ataatgattc aaaccgttgg cgcgtaaatt
    16981 acatgccgca caatgaccgc aaccgtcgcc tttaatgccg ttatagcagg tcaacgtttc
    17041 gttacggact aaatccagtt tgccgtaata atctgccagc gcccaggttt ccgctttatc
    17101 aatccacatc agcggcgttt caaaacgaat atctttcgcc atgcccaaac tgacggcatg
    17161 gtttagtgct ttcacaaact catcgcggca atccgggtag ccggagaaat ccgtttcaca
    17221 gacgccggta attacggctt ctgcttttac ctgatacgca tatattgccg ccagcgtcag
    17281 gaacaaaata ttacgccctg ggacaaacgt attcgggatg ccatcggctt caggttcata
    17341 atcaggcacc ggaatgctgt cacgtgtcag gctgctgacc gccagctcgt tgagcagcgt
    17401 tacatcaaga accttatgcg cgcgtgctcc cagtttcagc gccagttcgc gtgccacgtc
    17461 gatttctgcg cgatgtcgct gaccgtaatc gaacgtcacg caatggactt catcatattg
    17521 ttgtaatgcc tgcaccagac aggtggtgga atcctggcct ccactgaaca caacgacagc
    17581 acgtttcata gataatcctg cttgaacaat aaaagcgtta tggtaacgcc tgcgattaac
    17641 ccggaccagc tattcattgc gacggcgcgg gaagccaggc ttcggtaaat tcaaaccagc
    17701 cgcgcggtgt cagtcgcaca ccgttcacgc cgggaggggc actaatgcga tagtgatagt
    17761 tgaacagcgg cgtcagcgtc gcgtctgcca ttaactggct aaaaaccgct ttcagggcat
    17821 taaatcggtt ttcctcatca ggcattactt gcaccgcgtc cagcgtcgat tgcagatgtg
    17881 catatgctgg agcgtcgaaa acatgtggcc atagtggatc gcaacgtagc cattgctcca
    17941 gagtatattc cggtgcttcg ccaattaatc tgtcgcccat catgaggtct gcgtgtgcca
    18001 gtagggtcgt gtcgtcccag ttttttgcgt tatgaaaaat aattgtgagt tcacagcctt
    18061 ccgctgccag tgtcgcctgt aggcgttctg ccatggtatg aagttctatc ggtaggtgat
    18121 aaaccagcgt caaggttttc ggtagtttga cttcatccgg tacttgccag tgcggaatag
    18181 tccagcctgg cagtaatgca tgactggcgg tgatcaggtt ttctccgact tctaacgttt
    18241 gtaataaacc ggattgatga ataatggaga tcacttttcg cgcctgccag agggagagtc
    18301 ggggactttt gcgcaacgtt aaatagcaaa aacctaaact gatgccacta ctgacctggc
    18361 tgacccgttg cagctcctcc ggtttgccga tggtgatttg cacgggatgc cgacaactgg
    18421 ttcccaaatc tttttcgaaa agcggcggag ttatccagta ctcaaccgct ttaagcagcg
    18481 gatgacgcaa atggtaataa tcatggctct ccaggcgcac cagctccgct gtgaattgtg
    18541 ttaagcgaaa aggaccagtg ccgatcagtg ggaattgcgg atgcgccaga tggctgcaat
    18601 agctcgccag ccggtgcgca agccagtaat cggggcgatg taaaaagaag gtcagacact
    18661 gcggatgggt gacttcaata cgcttcacgc taataaataa ttgatccagt gctggcagtt
    18721 gtaacagcat caataatcgc tggtgtaagt gtgaggcttt tactgcatcg ccgttatgcc
    18781 agtgtagggt tgaacgaaga taaaagtccc agcgtaaccc gtcagtagag gtttcccagt
    18841 gatgcgctaa atcgccaatc gggcgctgag tattattatc gaagcgggtc aggccggaaa
    18901 atatctgccc ggcgagatgc tgctcggcac ggccgggcaa aaagcctggt tgtagcggtt
    18961 cgagcgggcg atagtaggga atacgcaacg tgggtgtatc gttttgccat tgtccgccca
    19021 taaacggctg taacagagtg cgtagctcac ctagggccag ttgcgccagc tccagcacat
    19081 cttgctgctt tccggtttcc agtgcctgtt ccatcatcgc attgcgtagc gattccggcg
    19141 tgaccagaaa gcgtaattgt ccgcgctttc cgcgtcctga ctgcgcctgc cactccagcc
    19201 atcccgcctc ctgtgcctga cgcaacagcg tacgaacatg gcgttcgctg caaaaacagc
    19261 gttcggccag ttcgctgacg gtgacggttt gcggctttcc ggctgaaggt tgccacagac
    19321 gttgatactg gttaagacgg ttgagcaatc gcatataaac ccggaacaat attatttaac
    19381 tattcactat tacttccgta tatatcaggt gatactcaat ctccattaac cgtgtcacag
    19441 agtggagaaa gaaatggctc gtctggcagc atttgatatg gatggcactt tattgatgcc
    19501 tgaccatcat ttaggtgaga aaaccctctc tactttggcg cgactgcgtg aacgcgacat
    19561 taccctcact tttgccacgg ggcgtcatgc gctggagatg cagcatattc tcggggcgct
    19621 atcgctggat gcatatttga ttaccggcaa cggaacgcgc gtgcattctc tggaaggtga
    19681 acttttacat cgtgatgatt tacctgcgga tgtcgcggag ctggtgctgt atcagcaatg
    19741 ggatacccga gccagcatgc atatcttcaa tgacgacggt tggtttaccg ggaaagagat
    19801 ccctgcgttg ttgcaggcat ttgtctatag cggttttcgt tatcagataa tcgatgtcaa
    19861 aaaaatgcca ctcggcagcg tcaccaagat ctgcttctgt ggcgatcacg acgatcttac
    19921 acgcttgcag atccagctat acgaagcatt aggcgagcgt gcacatttgt gtttttccgc
    19981 cacggattgc ctcgaagtgc tgccggtggg ctgcaataaa ggcgctgcat tgacggtgct
    20041 gacccaacat ctaggtttat cgttgcgcga ttgcatggcc tttggtgatg cgatgaacga
    20101 tcgcgaaatg ttaggcagcg tcggtagcgg gtttattatg ggcaatgcga tgccgcaact
    20161 gcgcgcggag ctcccgcatt taccggtgat tggacattgc cgaaattagg ctgtctctca
    20221 ctatttgacg cactgactgg actatccaca tctaccttat tcccccgaat aatgagatcc
    20281 cttccagcac cgggcaattg cccggttttt tttgcgttga atttgtcatt ttgtgccgtg
    20341 gtgtttaaac cgcacagaat aaattgtcgt gatttcacct ttaaaataga attaaaagag
    20401 aaaaaaattc tctgtggaag ggctatgtta gataaaattg accgtaagct gctggcctta
    20461 ctgcagcagg attgcaccct ctctttgcag gcactggctg aagccgttaa tctgacaacc
    20521 accccttgct ggaagcgcct gaaacggctg gaggacgacg gtatccttat cggcaaagtc
    20581 gccctgctgg atccggaaaa aataggcctc ggcctgaccg cctttgtgct gataaaaacg
    20641 caacatcaca gtagcgaatg gtattgccgc tttgtcacgg tggttaccga aatgccagaa
    20701 gtgctggggt tctggcgcat ggctggtgaa tacgattatc tgatgcgcgt ccaggttgcc
    20761 gacatgaaac gctacgacga gttttataag cgtctggtaa acagcgtgcc ggggctgtcg
    20821 gacgtcactt ccagcttcgc gatggaacag attaaataca ccacttcttt acccatcgaa
    20881 taaatatcca gaatcaggtc aggacacaac gcgtgcgatt atttgctcaa ttaagctggt
    20941 atttccgtcg ggaatggcgt cgctatctcg gggctgtcgc cttgcttgtc attatcgcga
    21001 tgctgcaact ggttccgcca aaagtggttg gtattgttgt cgatggcgtg acagaacaac
    21061 actttactac cgggcagatc ctgatgtgga tcgccaccat ggtgctgatt gccgttgtgg
    21121 tttatttcct gcgttacgtc tggcgggtat tgctgtttgg tgcgtcttat caactggctg
    21181 ttgaactgcg tgaagattat taccgtcagc taagccggca gcatcctgag ttttacctgc
    21241 gtcatcgcac gggtgacctc atggctcgtg cgaccaatga cgtcgatcgc gtcgtgtttg
    21301 cagccggaga aggggtgctg acgctggtgg attcactggt gatgggctgc gctgtgttga
    21361 ttatgatgtc tacgcaaatt agctggcagt tgaccttatt tgccctgttg ccgatgccag
    21421 tgatggcgat catgatcaag cgcaacggcg atgccttgca tgaacgcttt aagctggcac
    21481 aggcggcgtt ttctagtctt aatgaccgca cccaggaaag cctcaccagt atccgcatga
    21541 tcaaagcctt tggtctggaa gatcgccagt cggcgttatt tgccgcggat gccgaagata
    21601 ccggcaaaaa aaacatgcgg gtggcgcgta ttgatgctcg tttcgacccg accatctata
    21661 tcgcgattgg tatggcgaat ttgctggcga ttggcggcgg tagttggatg gtggtgcagg
    21721 gcagtttaac gctgggccag ctcaccagtt ttatgatgta tttaggtctg atgatttggc
    21781 caatgctggc gctggcatgg atgtttaaca ttgtggaacg tggtagtgct gcatacagcc
    21841 gtattcgcgc gatgctggcg gaagcgccgg tggtgaacga tggtagcgaa cccgtgccgg
    21901 aagggcgtgg cgaactggat gtaaatattc accagttcac gtatccgcag actgaccatc
    21961 ctgcgctgga aaacgtcaat ttcgccctga aacccggtca gatgctgggt atctgcgggc
    22021 cgactggttc cggcaaaagt accctgttgt cgctcattca gcgtcatttc gacgtcagcg
    22081 agggggatat tcgctttcat gatattcctc tgacgaagtt acaactcgat agctggcgta
    22141 gccgcctggc ggtagttagc cagacaccgt tccttttttc cgacaccgta gcgaacaaca
    22201 tcgcgttggg ttgcccgaat gccacccagc aagagattga gcatgtcgcg cggttagcca
    22261 gcgtacatga cgatattttg cgtctaccgc aaggttacga tacagaagtg ggcgagcgcg
    22321 gcgtgatgct ttccggtggg caaaaacagc gtatctccat tgctcgagcg ttattagtca
    22381 acgcggaaat cctcatcctt gatgatgcgc tttcggcggt ggacggacgc acagagcacc
    22441 agatcctgca taacctgcgt cagtggggac agggaagaac ggtaatcatc agtgcccatc
    22501 gcctttcggc actgacggaa gccagtgaaa atattgtgat gcagcacgga catatcgccc
    22561 agcgtggcaa tcatgatgag ctggcgcaac aaagcggctg gtatcgcgat atgtatcgct
    22621 atcaacaact ggaggcggcg ctcgacgacg ctccggaaat tcgcgaggag gccgtcgatg
    22681 cgtagtttta gccaactgtg gccgactctc aagcgtctgt tagcgtacgg ttcgccgtgg
    22741 cgtaaaccgc tggggattgc ggtcctgatg atgtgggttg cggcagcggc agaagtcagt
    22801 gggccgctgc ttatcagcta ttttatcgac aatatggtag cgaaaaataa cctgccgttg
    22861 aaaatggttg cagggctggc tgcggcgtat gttgggctac aactgtttgc cgccgggcta
    22921 cattacgcgc agtcgctgct gtttaatcgg gcggcagtag gcgtagtgca acagttgcgt
    22981 accgacgtga tggatgccgc attgcgccag ccattaagcg agtttgatac ccaacccgtc
    23041 gggcaggtga tttcccgcgt cactaatgac actgaagtga tccgcgatct ctacgttacc
    23101 gtagtggcaa ctgtcctgcg tagcgccgca ctggtgggcg cgatgctggt ggcgatgttc
    23161 agcctcgact ggcgaatggc gctggtggcg ataatgattt tcccggtggt gctggtggta
    23221 atggtgatat accagcgtta cagcacgccg attgtccgtc gtgtgcgcgc ctatctggcg
    23281 gatatcaacg acggctttaa cgaaatcatc aatggcatga gcgttatcca gcagttccgt
    23341 cagcaggcgc gatttggcga acgtatgggg gaggccagtc gttcacacta tatggccagg
    23401 atgcaaaccc tgcgcctcga cggttttctg ctgcgtccgc tgctgagtct gttttcatcg
    23461 ctcattcttt gtggcttgtt gatgctgttt ggcttctccg ctagcggcac cattgaagta
    23521 ggcgtgctgt atgcgtttat cagctatctt gggcgactta acgaaccctt aatcgaactg
    23581 accacgcaac aggcgatgct gcaacaggct gttgttgctg gtgagcgcgt gtttgaactg
    23641 atggacggac cgcgccagca atatggcaat gatgatcgcc cgttacagag tggcaccatc
    23701 gaagtcgata acgtgtcatt tgcttatcgc gatgacaatc tggtgctaaa gaacattaat
    23761 ctctctgtgc cttcgcgtaa ctttgtggca ctggtcgggc ataccggcag tggcaaaagc
    23821 accctcgcca gtttattgat gggctattac ccgctaacgg aaggtgagat tcgccttgat
    23881 ggtcgtccat taagttcgct aagtcacagc gcgctgcgcc agggcgtggc aatggtgcag
    23941 caagatccgg tggtgctggc ggataccttc ctcgccaacg tgacgctggg gcgtgatatc
    24001 tccgaagaac gcgtctggca ggcgctggaa accgtgcaac tggcggagct ggcgcgcagc
    24061 atgagtgatg gtatttatac gccgctgggc gagcagggga ataatctctc cgtcgggcaa
    24121 aagcaactgc tggcactggc acgcgtgttg gtggagacgc cgcaaatcct gatccttgat
    24181 gaggcaaccg ccagcattga ctccggtact gaacaggcga ttcaacatgc tctggcggct
    24241 gtgcgtgaac ataccacgct ggtggtgatt gctcaccgct tatcgaccat tgttgatgcc
    24301 gacaccattc tggtgcttca tcgtgggcaa gccgtggagc agggcactca ccagcaactg
    24361 ctggcggccc agggacgcta ctggcagatg tatcaactgc aacttgcggg cgaagagctg
    24421 gcagccagcg tgcgtgaaga agaatcattg agcgcctgaa tagcgcaata tttcatcgtt
    24481 ggtgcaaaaa tgtaacgcac tgtgcactgt catagtgcgt tttcattttc aatcttctta
    24541 acttcctgct ctctttctcg tttttcattt ctggcacacc gcttgcaata ccttcttcgt
    24601 gtagcagaac cattaccgaa ttctgactgg aggggatcta tgaagctggt gaccgtgata
    24661 atcaaaccat tcaagctgga agacgttcgt gaagcgttat cttccattgg tattcagggc
    24721 ctgaccgtca ccgaagtgaa aggtttcggg cgtcagaaag ggcatgccga gctgtaccgg
    24781 ggggcggaat acagcgtcaa cttcctgcca aaagtaaaaa ttgatgtggc aattgctgat
    24841 gaccaactcg atgaagtgat cgatatcgtc agtaaggcgg cttacaccgg aaaaattggc
    24901 gacggcaaaa tcttcgtcgc tgaattgcaa cgcgtcattc gtattcgtac cggcgaagcc
    24961 gacgaagcgg cgctgtaatc tctggcacac agcaacagga acgaaaaatg aagatagcga
    25021 cgataaaaac tgggcttgct tcactggcga tgctgccggg actggtaatg gctgcacctg
    25081 cggtggccga taaagccgac aatgcgttta tgatgatttg tactgcgctg gtgctgttta
    25141 tgactattcc ggggattgcc ctgttttacg gtgggttgat tcgcggcaaa aacgtgctgt
    25201 cgatgctgat gcaggtgacg gtgacgtttg cactggtctg tattctctgg gtggtttacg
    25261 gttactcgct ggcgtttggt gagggtaaca acttcttcgg caacgttaac tggttgatgc
    25321 tgaaaaacat cgaactgacg gcggtgatgg gcagcattta tcagtatatc cacgtagcgt
    25381 ttcagggatc gtttgcctgc attaccgtag gcttgatagt tggggcgctg gcggaacgaa
    25441 tccgcttctc agctgtgttg attttcgtgg tggtatggct gacgctctct tacattccga
    25501 ttgcgcatat ggtgtggggc ggcggtttgc tggcttctca cggtgcactg gatttcgcgg
    25561 gtggcacagt ggtgcacatt aacgccgcaa tcgccggtct ggtgggcgcg tatctgatag
    25621 gcaaacgcgt gggcttcggt aaagaggcgt ttaaaccgca caacctgccg atggtcttca
    25681 ccgggactgc cattctctat atcggttggt ttggctttaa cgccgggtca gcgggcacgg
    25741 cgaatgaaat cgcggcactg gcatttgtga atactgtggt cgcaacggcg gcggcaattc
    25801 ttggctggat cttcggtgaa tgggcgctgc gtggtaagcc ttcactgctg ggggcgtgtt
    25861 ctggcgcgat tgccggtctg gtcggcgtga cgccagcgtg cggctacatt ggggttggcg
    25921 gcgcgttgat tatcggcgtg gtagctggtc tggcgggctt gtggggcgtt accatgctca
    25981 aacgcttgct gcgggtggat gatccctgcg atgtcttcgg tgtgcacggc gtttgtggca
    26041 ttgtcggctg tatcatgacc gggatttttg ccgccagctc gctgggcggc gtgggcttcg
    26101 ctgaaggtgt gacgatgggt catcagttgc tggtacagct ggaaagcatc gccattacga
    26161 tcgtctggtc cggtgtggtg gcatttattg gctacaaatt ggcggatctg acggttggtc
    26221 tgcgtgtacc ggaagagcag gagctagaag ggctggatgt caacagtcac ggcgagaatg
    26281 cctataacgc gtaacaagca ctgcaaaaaa cagccggacg gttttcacct ccggctattt
    26341 ttttaattgt gattacgcat caccccttcc tgaacggtcg aggcaaccag tacgccgtcc
    26401 tgggtataaa actcaccgcg cacaaagcca cgtgcgctgg acgccgaggt gctctccacg
    26461 ctatacagca gccattcatt caaattaaac gggcgatgga accacatgga atggtcaatg
    26521 gtggcaatct gaatccccgg ttcgagaaaa ccgatgccgt gcggctgtag agctaccggc
    26581 aggaagttaa gatcagaagc gtaaccgagc agatactgat gaacgcgcag gtcatccggc
    26641 acgctaccat ttgcgcggat ccacacctga cgatgtggtt ctgcgacgtg acctttcagt
    26701 gggttatgaa actccaccgg acggacttcc agcggacgat cgcagatgaa tttatcttta
    26761 agcactggcg gcagcaggtg cgccagcgat tgggcgattt gcgtttccga aggcaggcca
    26821 tcaggcgctg gcgcggacgg cattgttttt tgatgttcga aacccgcttc tggtgcctgg
    26881 aaagaggccg tcatataaaa aatcggtttg ccgttttgaa tagcagcaac ccggcgggcg
    26941 ctgaagctgt taccatcacg cagcgtttcg acatcataaa taatcggttt cttactatcg
    27001 cctgggcgaa gaaagtagct gtgaaacgaa tgtaccagcc gctcttccgg gacggtctct
    27061 tttgcagcat acaaggcctg acccacgacc tggccgccaa acacctggcg taaacctaaa
    27121 tcttcactct ggccgcgaaa gagtcctgcc tcaatttttt ccagatttaa caatgccagt
    27181 aaatttttta gcgcctgact catataactc tccagtaaca aagctgccgc agcaagccaa
    27241 agtgagttga gtataacgca aatttgccac tggtccgatg ggtgcaataa tctgaattac
    27301 gggctaatta caggcagaaa tgcgtgatgt gtgccacact tattgacgtt actattttgt
    27361 taaccactct tccggcgagg aaagttagcc cgctggtgca ttgataataa ggagaaatga
    27421 atgaaactcg tgcacatggc cagtggttta gcggttgcga ttgcgttggc ggcttgcgca
    27481 gataaaagcg cggatattca gacgccagcc ccggctgcaa atacgtctat ttcagcaaca
    27541 caacaacctg ctatccagca accgaatgtc tccggtaccg tctggatccg gcagaaagtc
    27601 gcactgccgc ctgatgctgt gctgaccgtg acactttctg acgcgtcgtt agccgatgca
    27661 ccgtcaaaag tgctggcgca gaaagcggtg cgtaccgaag gtaaacagtc accattcagc
    27721 tttgttctgc catttaaccc ggcagatgtt cagccgaacg cgcgtattct gttgagtgcg
    27781 gcgattaccg tgaatgacaa actggtattt attaccgata ccgttcagcc ggtgatcaac
    27841 aagggcggaa ctaaagccga cctgacattg gtgccggtgc agcaaaccgc cgtgccggtt
    27901 caggccagcg gtggtgcaac gactaccgta ccttcgactt caccaactca ggtgaatccg
    27961 tcttcggcag ttccagctcc tacgcaatat taagcgacgt taaccctctc c
//
LOCUS       DAJTNN010000034        27997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTNN010000034 DAJTNN010000000
VERSION     DAJTNN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.034"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(206..1306)
                     /gene="chaA"
                     /locus_tag="OO150_001330"
     CDS             complement(206..1306)
                     /gene="chaA"
                     /locus_tag="OO150_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5515532.1"
                     /translation="MSNAQEAVKTRHKEISLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1576..1806
                     /gene="chaB"
                     /locus_tag="OO150_001331"
     CDS             1576..1806
                     /gene="chaB"
                     /locus_tag="OO150_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5515533.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1964..2659
                     /gene="chaC"
                     /locus_tag="OO150_001332"
     CDS             1964..2659
                     /gene="chaC"
                     /locus_tag="OO150_001332"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5515534.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="OO150_001333"
     CDS             complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="OO150_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5515535.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3240..4634
                     /gene="ychO"
                     /locus_tag="OO150_001334"
     CDS             3240..4634
                     /gene="ychO"
                     /locus_tag="OO150_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5515536.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYFTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLLANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEYNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4635..5285)
                     /gene="narL"
                     /locus_tag="OO150_001335"
     CDS             complement(4635..5285)
                     /gene="narL"
                     /locus_tag="OO150_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5515537.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5278..7074)
                     /gene="narX"
                     /locus_tag="OO150_001336"
     CDS             complement(5278..7074)
                     /gene="narX"
                     /locus_tag="OO150_001336"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5515538.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRVRLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7413..8804
                     /gene="narK"
                     /locus_tag="OO150_001337"
     CDS             7413..8804
                     /gene="narK"
                     /locus_tag="OO150_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5515539.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9320..13042
                     /locus_tag="OO150_001338"
     CDS             9320..13042
                     /locus_tag="OO150_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5515540.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            13060..14598
                     /gene="narH"
                     /locus_tag="OO150_001339"
     CDS             13060..14598
                     /gene="narH"
                     /locus_tag="OO150_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5515541.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14595..15305
                     /gene="narJ"
                     /locus_tag="OO150_001340"
     CDS             14595..15305
                     /gene="narJ"
                     /locus_tag="OO150_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5515542.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEVIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15305..15982
                     /gene="narI"
                     /locus_tag="OO150_001341"
     CDS             15305..15982
                     /gene="narI"
                     /locus_tag="OO150_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5515543.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFAGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16165..16296)
                     /locus_tag="OO150_001342"
     ncRNA           complement(16165..16296)
                     /ncRNA_class="other"
                     /locus_tag="OO150_001342"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(16344..16428)
                     /locus_tag="OO150_001343"
     tRNA            complement(16344..16428)
                     /locus_tag="OO150_001343"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16392..16394),aa:Tyr,seq:gta)
     gene            complement(16463..16547)
                     /locus_tag="OO150_001344"
     tRNA            complement(16463..16547)
                     /locus_tag="OO150_001344"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16511..16513),aa:Tyr,seq:gta)
     gene            complement(16707..17549)
                     /gene="purU"
                     /locus_tag="OO150_001345"
     CDS             complement(16707..17549)
                     /gene="purU"
                     /locus_tag="OO150_001345"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5515544.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(17599..18057)
                     /locus_tag="OO150_001346"
     CDS             complement(17599..18057)
                     /locus_tag="OO150_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5515545.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            18170..19075
                     /gene="rssA"
                     /locus_tag="OO150_001347"
     CDS             18170..19075
                     /gene="rssA"
                     /locus_tag="OO150_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5515546.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQCREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRVLGADIVIAVDLQHDAHLIQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            19167..20180
                     /gene="rssB"
                     /locus_tag="OO150_001348"
     CDS             19167..20180
                     /gene="rssB"
                     /locus_tag="OO150_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5515547.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            20382..21290
                     /gene="galU"
                     /locus_tag="OO150_001349"
     CDS             20382..21290
                     /gene="galU"
                     /locus_tag="OO150_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5515548.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(21434..21847)
                     /gene="hns"
                     /locus_tag="OO150_001350"
     CDS             complement(21434..21847)
                     /gene="hns"
                     /locus_tag="OO150_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5515549.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            22452..23069
                     /gene="tdk"
                     /locus_tag="OO150_001351"
     CDS             22452..23069
                     /gene="tdk"
                     /locus_tag="OO150_001351"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5515550.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(23351..24247)
                     /locus_tag="OO150_001352"
                     /pseudo
     CDS             complement(23351..24247)
                     /locus_tag="OO150_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(24371..27046)
                     /gene="adhE"
                     /locus_tag="OO150_001353"
                     /gene_synonym="adhC"
     CDS             complement(24371..27046)
                     /gene="adhE"
                     /locus_tag="OO150_001353"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5515551.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGILSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            27524..>27997
                     /gene="ychE"
                     /locus_tag="OO150_001354"
     CDS             27524..>27997
                     /gene="ychE"
                     /locus_tag="OO150_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5515552.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIA"
BASE COUNT         6891 a         6735 c         7303 g         7068 t
ORIGIN      
        1 ctcaattcat cttcacttca tccctgaaat gtttgcaata aagagtacat tccggctttt
       61 caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt ctgattaacg
      121 agctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatcttatc tcccccgatt
      181 tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag gcggcaaaca
      241 gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag gagatatggc
      301 acagcactaa agaggccacc atcaccacca tttctggcgc accaagtgca aactgtaatt
      361 cgttacccgt cataaaggca attagcgtga cgacaggtac ggtaagtgaa atggttgcta
      421 acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttgttcaac actgctttta
      481 acgcacctaa accttccggc gacagaatca acagagccac caggaagcca gtaaaggcga
      541 caggggcatt catgctgtcg agcaatgtct ccagcgggct ggcattcatt ttggtgactg
      601 caataacggc aatcagatgg ataatcaacc agatagcatg ccacacgctg ctatgggcag
      661 acggtttacc gtgatgcggg tcgtcgtcat cactgtcatc ttcgtgctcg tagacaaaca
      721 aactttgatg cgttttggtc tggatcagca aaaatacgcc atacattgcc gcagaaatta
      781 atgctaccag taacgcctga ccggttgaaa aattcgccgc aggcagagcc attggaaata
      841 ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttgata ccaaacagat
      901 tcatatattg ggtggcaaac ttacgaccgc ccaacaataa tgaaaagcca accagcccac
      961 cggtaacaat cataatgatt gaatagagcg tatcacgcat tagcgttggc gcggcgtcgc
     1021 cggttgccat taaagctgaa atcaaactga cttcaagaat aaccactgaa aggctaagaa
     1081 taagcgaacc gtaaggttct cccaggcgat gggctaatac gtccgcatga cggacaacac
     1141 taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg accactggta
     1201 gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg aaaataagcg
     1261 aaatctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg gttatccctt
     1321 tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa aaataacaga aagtgtctgg
     1381 atatcggtaa cattttacga atttttaccc cactgtcgat tttttactca ttgggacata
     1441 aaaataagta ttacgtttag acaatgtttg ttttagcgtt tcttaagaac agtctgacat
     1501 gaaaattctt atgttttggc aagagtagat attgttgacc acacttaatg ttcaactttg
     1561 taaaaggagt caacgatgcc gtataaaacg aaaagcgatc tgccggaaag cgtaaagcac
     1621 gttctaccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc atgggatcaa
     1681 tataaagata aagacgatcg gcgtgatgac gccagtcgcg aagaaaccgc gcataaagtg
     1741 gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg gcataaaaaa
     1801 tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca agtggtccgt
     1861 ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc cggaagacac
     1921 caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt aaggtgataa cgcgtgattt
     1981 cttgatgaat gccgattgta aaacggcatt tggtgccatt gaagaatcac tcttatggtc
     2041 agcagagcaa cgagcggctt cgctggcggc gacgctggct tgtcgacctg atgagggacc
     2101 ggtgtggatc ttcggttatg gatcgttgat gtggaatccg gcactggagt ttaccgagtc
     2161 gtgcaccggt acactggttg gatggcatcg cgcgttttgc ctgcgcctga ccgccgggcg
     2221 cgggactgcg caccagccgg gacggatgct tgcactgaaa gagggcggac gcactacagg
     2281 cgtcgcctat cgactgccag aagagacgct ggagcaggaa ctaaccctgt tgtggaagcg
     2341 cgagatgatt accggctgtt atctgccaac ctggtgtcag cttgatcttg atgatggatg
     2401 cacagtaaac gccattgtgt ttattatgga cccgcgacat ccagaatatg aatctgacac
     2461 tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc ggtccgctgg gaaccaacgc
     2521 acaatacctg ttttcactgg aacaggagct tatcaaactg ggaatgcagg acgatgggct
     2581 gaatgatttg ctggtatcgg taaaaaaact gctggcggag aattatccgg atggtgtgtt
     2641 acggccggga ttcgcctgag taaacttccc gcatagtggg gcgtcagacg ccccctcaaa
     2701 cattaaaatg tgagcacttt atcggctgac agcgtccatt gcgccagttc cacaagagta
     2761 ccgatttcca ccccatcaat cagaggaagt gtactaatcc cgcgcccgtc ggtacaggtt
     2821 ttgcacaatt tcaccggtac attctgagcg gtaaggatct ccagcatttg ctgaatgttg
     2881 tagccttccc ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga catgaggaac
     2941 agacgcagat ccagattgct ctcctgctct cgtaacgcaa tggccagccg caagctgtta
     3001 aacaaggatt cgctcccgta aggtgcgcca ttggcaacga tcacgatttt ttgcattatt
     3061 tactcctgta ttcagggaat tagacactca tcttctatct tactgcttct gcagcgtctg
     3121 accaatcggt cacattttta aggattttcc tgaaagcgcg agaaaatacg acaaaagttg
     3181 ccagtaatcg ttattcttta aggctatggt tttcattatt accggaagtt accgacgttt
     3241 tgagccgttt cgttcctcgc attatcccgt tttatttact cttgcttgta gcaggcggta
     3301 cagctaacgc acaatctacc ttcgagcaaa aagcggcaaa tccctttgat aataacaatg
     3361 atggtctgcc ggatttaggc atggctcccg aaaatcatga tggggaaaaa cactttgctg
     3421 aaattgtgaa agatttcggc gaaaccagta tgaatgataa cgggctggat actggcgagc
     3481 aggcaaaagc tttcgcattg ggaaaagtcc gcgacgcgct tagtcaacag gttaatcagc
     3541 acgtagagtc ctggctatca ccgtggggaa atgccagtgt tgacgtcaaa gtggataacg
     3601 aaggacattt caccggcagt cgtggaagct ggtttgtgcc gttacaagat aatgatcgtt
     3661 atttcacctg gagccagctt ggtcttactc agcaggataa tgggttggtg agcaatgtgg
     3721 gcgttgggca acgctgggcg cgcggcaact ggctggtggg ttataacact ttttatgaca
     3781 acttgctgga cgaaaatctt cagcgagcgg gctttggtgc cgaagcgtgg ggcgaatatt
     3841 tgcgattatt ggcaaacttt tatcagccgt ttgctgcatg gcatgaacag acagccacgc
     3901 aggaacaacg gatggcgcgc gggtacgacc tgacagctcg gatgcgcatg ccgttctatc
     3961 aacacctcaa taccagtgtc agcctagaac agtattttgg tgatcgtgtt gatttgttta
     4021 actctggtac gggttatcac aatcccgtcg cgttgagtct gggattaaat tacacccctg
     4081 tgccattagt cactgtgacg gcccagcata aacagggtga aagtggcgag aatcaaaata
     4141 acctcgggct gaatcttaat taccgctttg gtgtaccgct caaaaaacaa ctttctgcgg
     4201 gcgaggttgc cgaaagtcag tcgttacgtg gtagtcgcta tgacaatccg cagcgaaata
     4261 atctaccgac tcttgagtac cgacagcgaa aaacgttaac ggtgtttctg gcgacaccgc
     4321 cgtgggatct aaaacctggc gaaacagtgc cgctgaaatt acaaatccgc agtcgttacg
     4381 gtattcggca actgatttgg cagggcgata cgcagatatt aagtttgacg ccgggcgcac
     4441 aagccaacag cgcggagggc tggacgctga tcatgcctga ctggcagaac ggggaagggg
     4501 caagcaatca ctggcgattg tcggtggtgg tggaatataa ccaggggcag cgtgtctcct
     4561 ccaatgagat cacgctaacg cttgtcgaac cgttcgatgc attgtcaaac gacgaactgc
     4621 gctgggaacc gtaatcagaa aatgcgctcc tgatgcaccc ataccgctgc ttccacgcga
     4681 gacttgagct tcattttctt cagcatgtgc ttgacgtgca cttttactgt gctttcggtg
     4741 atatccaggc ggcgggcaat catcttgttc ggcaaaccct gggcaatcag cttgagaata
     4801 tcgcgctcgc gtggggttaa ctggttaaca tcgcgctcag tagtggcacg gttagcgcgc
     4861 aagctggcgg ccagaacagg cgttaatgct tcgcttaata ccatttcgcc agcagctgcc
     4921 tgatgcaatg ctttcagcag atcttccggt tccatatctt ttaacagata accatccgcg
     4981 ccgcgtttca gtgcggtgac cacatcttct tcatggttag agacgctgaa taccacaatg
     5041 cgccctgaga gggacttttc gcgcagttta tccagcgttt ccagaccgtt catgccgggc
     5101 atattgagat ctaacaggat cagatcggga tcaagagact ccgccagttc aataccctgt
     5161 tcgccattac tcgcttcgcc aaccacggtg atatctggtg ccatactgat aagctgtttt
     5221 acgccagttc gcagcatcgg gtgatcgtcg atcagcagga tagtagccgg ttcctgatta
     5281 ctcatgggta tctccttgga cgtctgtgaa agttttttcg ggaataaagg tgaccaccac
     5341 ttcggtgcca cctgattcac gacggcggac gcggcaatcg cctcgtaaac tttgcgcacg
     5401 gtcgcgcatt attatcatgc cgtagtgatt gctgcggatg gcattttcag gcacgccgca
     5461 gccgttatct tggacggtca gtttgacctg attatcgttt tgcgccaccg tcaccacgac
     5521 ctcactcgct tgcgaatgtt tgagggcgtt acttaatgcc tcacgggcaa tttgcaacaa
     5581 gtggattgcc tgatgtgaag gcaccagacg aggcggcaat tgataatcca gcttcaccgg
     5641 gaagccaaat ttggcgctgt actcttcgca actcgcctcc agcgccggac gtaatccagg
     5701 ctcggtgagc tgcaagcgga atgtggtgag caattcacgc aactgcgccc aggatgcatt
     5761 cagttcgtta cggatctgac ttaacagttc gcggctgctt gctggcagcg catcgccctg
     5821 catctgtaaa caactcacct gcatcttcat gcaagagaga gattgggcaa tagaatcatg
     5881 cagttcgcgc gcaatggtgg cacgctcttc catcacgatc aactgttgct gacgttcctg
     5941 atggcgatcc agcgccagcg tggcggtgag ttgttcaacc agggtatcca ccagttgttg
     6001 ttgatcatgg ctaagatgac gcccctgcgg cagggtcgcc agcaaaatac cgtactgcgt
     6061 atgagagtca gccagccgcc acttcagggt tgtgccgcga tcaccaacgg gtaatacgcc
     6121 gcgcgggcag agctggcagc ctttatcatc acaagtcata tctggctggc aggtaaactc
     6181 ctgatgattc tcttcatcat cagtgtcata cacccgcagt tcgatatcac gtagcagggt
     6241 taaattctgt aagccgttga gtacaggtga caggcgttca cacagcgggg cgcgggaatg
     6301 caaacggcgg ttagcctgcc ataaaaaaga gaggatctga tttttatgct ccagcccggc
     6361 ggttttctcc tgaacccgct gctcaagtac ggcataactt tcggccagtt ctgcagacat
     6421 attgttcaat gcagttccaa gcatcgccat ttcgttgcgc ccgctgatgt tcgcgcgttg
     6481 ggtaaaatcg cgatgactga cggcactcgc cattgccagc agttgccgcc acggttgtag
     6541 cagtcgcacc cgcaaccaga taatagtgaa caccagtaaa agtgccataa ataccgccat
     6601 tacccgatgg accagtacca ctgtctcaat gcgcatttcc gtggtgcggt caaaaccaga
     6661 taccagctga tcaagcccgg caacaaactg gctgacatcc gctgacaccg tttcgcggtt
     6721 ttgtgcacgc atcagtgcag ggatcagttc attacgccag taatcttgta aaccctgtaa
     6781 ttgcgccagt tgttcgtctc gttctgctgc tcgagttaac tcggcgctaa atgccgtttg
     6841 ttccatctct ttaattaagg gcttgtcttt ctcacttaat ggcactgccg ccaacagacg
     6901 gtaactttgc atgcgcagcg atcccgcttt gttgatcgca tgggcgctgc cctgaacgcc
     6961 ttgcaccagc cagccagaaa ccgccatccc tgccagtcca atagcagtag aaagcaacac
     7021 aataagcgca acctgattaa ccagggtgag cggagagaga caacgtttaa gcatgtaaac
     7081 ctcttccttc aggctttaaa tgagcaataa ccttaatgaa tgtgacgata cattctggaa
     7141 tggcagtatt ctcggctatt ggctgaagta tacccatacc cggaaagagt tactccttat
     7201 ttgccgtgtg gttagtcgct ttacatcggt aagggtaggg attttacagc accgtgaaaa
     7261 atctcataat ttttatgaaa tcactgtact cactatgggt aatgataaat atcaatgata
     7321 gataaagtta tcttatcgtt tgatttacat caaattgcct ttagctacag acactaaggt
     7381 ggcagacatc gaaacgagta tcagaggtgt ctatgagtca ctcatccgcc cccgaaaggg
     7441 ctactggagc tgtcattaca gattggcgac cggaagatcc tgcgttctgg caacaacgcg
     7501 gtcaacgtat tgccagccgt aacctgtgga tttccgttcc ctgtctgctg ctggcgtttt
     7561 gcgtatggat gttgttcagc gctgttgcgg tgaacttacc gaaagtcggt tttaatttta
     7621 cgaccgatca gctatttatg ttgactgcgc tgccttcggt ttctggcgcg ttattacgtg
     7681 ttccatactc ctttatggtt cctatcttcg gtggtcgtcg ctggacggcg ttcagcaccg
     7741 gtattctgat tattccttgc gtctggctgg gttttgccgt gcaggatacc tccacgcctt
     7801 atagcgtctt catcatcatc tctctgctat gcggctttgc tggcgcgaac ttcgcatcca
     7861 gtatggcaaa catcagcttc ttctttccga aacagaagca gggtggcgcg ctgggtctga
     7921 atggtggtct gggaaacatg ggcgtcagcg tcatgcagtt ggttgctccg ctggtggtat
     7981 cactgtcgat tttcgcagta tttggtagcc agggcgtcaa acagccggat gggactgagc
     8041 tgtatctggc gaatgcgtcc tggatatggg tgccgttcct tgccatcttc accattgcgg
     8101 cgtggtttgg catgaacgat cttgctacct cgaaagcctc catcaaggag cagttgccgg
     8161 tactcaaacg gggtcatctg tggattatga gcctgctgta tctggcaacc ttcggctcct
     8221 tcatcggctt ctccgcgggc tttgcaatgc tgtcaaaaac gcagttcccg gatgttcaga
     8281 ttctgcaata cgctttcttc gggccgttta ttggtgcgct ggcgcgttct gcaggtggtg
     8341 cattatctga ccgtctgggc ggaactcgtg tcacgctggt gaactttatt ctgatggcga
     8401 ttttcagcgg cctgctgttc ctgaccttac cgactgacgg gcagggcgga agcttcatgg
     8461 cgttcttcgc ggtcttcctg gcgctgttcc tgacagctgg gctgggtagt ggttccactt
     8521 tccagatgat ttcagtgatc ttccgtaaac tgacaatgga tcgcgtgaaa gcagaagggg
     8581 gttctgacga acgtgcgatg cgtgaagcgg caaccgacac ggcggcggcg ctgggtttca
     8641 tctctgcgat tggcgcgatt ggtggcttct ttatcccgaa agcgtttggt agctcgctgg
     8701 cattaacggg ttcgccagtc ggcgcaatga aggtattttt gattttctat atcgcctgcg
     8761 tagtgattac ctgggcggta tatggtcggc attctaaaaa ataaaccgtt actcgtcata
     8821 ctccgggtta catgtgctgc ggctgcgttc attcacccca gtcacttact ttagtaagct
     8881 cctgggattc attcacttgc cgccttcctg taaaccgaat tatatagagt aaaatatttg
     8941 attatccttt gcgcggcatg atgtcgcgct ttttttaagc gttatttagt tacaacatac
     9001 taatgttata tggtttattt cgccggattt cattaagagc cattaatatg ttacccatgg
     9061 ggaatactcc ttaataccca tctgcataaa aatcttaata gtttaaataa ctacaggtat
     9121 aaaacgtctt aatttacagt ctgttatgtg gtggctgttg attatcctaa aggggtatct
     9181 taggaattta ctttattttt catccccatc actcttgatc gttatcaatt cccacgctgt
     9241 ttcagagcgt taccttgccc ttaaacatta gcaatgtcga tttatcagag ggccgacagg
     9301 ctcccacagg agaaaaccga tgagtaaatt cctggaccgg tttcgctact tcaaacagaa
     9361 gggtgaaacc tttgccgatg ggcatggcca gcttctcaat accaaccgtg actgggagga
     9421 tggatatcgc cagcgttggc agcatgacaa aatcgtccgc tctacccacg gggtaaactg
     9481 caccggctcc tgcagctgga aaatctacgt caaaaacggt ctggtcacct gggaaaccca
     9541 gcagactgac tatccgcgta cccgtccgga tctgccaaac catgaacctc gcggctgccc
     9601 gcgcggtgcc agctactcct ggtatcttta cagtgccaac cgcctgaaat acccgatgat
     9661 gcgcaaacgc ctgatgaaaa tgtggcgtga agcgaaggcg ctgcatagcg atccggttga
     9721 ggcatgggct tctatcattg aagacgccga taaagcgaaa agctttaagc aggcgcgtgg
     9781 acgcggtgga tttgttcgtt cttcctggca ggaggtgaac gaactgatcg ccgcatctaa
     9841 cgtttacacc atcaaaaact acggcccgga ccgtgttgct ggtttctcgc caattccggc
     9901 aatgtcgatg gtttcttacg catcgggtgc acgctatctc tcgctgattg gcggtacttg
     9961 cttaagcttc tacgactggt actgcgacct gcctcctgcg tctccgcaaa cctggggcga
    10021 gcaaactgac gtaccggaat ctgctgactg gtataactcc agctacatca tcgcctgggg
    10081 ttcaaacgtg ccgcagacgc gtaccccgga tgctcacttc tttactgaag tgcgttacaa
    10141 aggcaccaaa actgttgccg tcacaccaga ctacgctgaa atcgccaaac tgtgcgatct
    10201 gtggctggca ccgaaacagg gcaccgatgc ggcaatggcg ctggcgatgg gccacgtaat
    10261 gctgcgtgaa ttccacctcg acaacccaag ccagtatttc accgactatg tgcgtcgcta
    10321 caccgacatg ccgatgctgg tgatgctgga agaacgcgac ggttactacg ctgcaggtcg
    10381 tatgctgcgc gctgctgatc tggttgatgc gctgggccag gaaaacaatc cggaatggaa
    10441 aactgtcgcc tttaatacca atggcgaaat ggttgcgccg aacggttcta ttggcttccg
    10501 ctggggcgag aagggcaaat ggaatcttga gcagcgcgac ggcaaaactg gcgaagaaac
    10561 cgagctgcaa ctgagcctgc tgggtagcca agatgagatc gctgaggtag gcttcccgta
    10621 ctttggtggc gacggcacgg aacacttcaa caaagtggaa ctggaaaacg tgctgctgca
    10681 caaactgccg gtgaaacgcc tgcaactggc tgatggcagc accgccctgg tgaccaccgt
    10741 ttatgatctg acgctggcaa actacggtct ggaacgtggc ctgaacgacg ttaactgtgc
    10801 aaccagctat gacgatgtga aagcttatac cccggcctgg gccgagcaga ttaccggcgt
    10861 ttctcgcagc cagattattc gcatcgcccg tgaatttgcc gataacgctg ataaaacgca
    10921 cggtcgttcg atgattatcg tcggtgcggg gctgaaccac tggtatcacc tcgatatgaa
    10981 ctatcgtggt ctgatcaaca tgctgatttt ctgcggctgt gtcggtcaga gcgggggcgg
    11041 ctgggcgcac tatgtaggtc aggaaaaact gcgtccgcaa accggctggc agccgctggc
    11101 gtttgccctt gactggcagc gtccggcgcg tcacatgaac agcacttctt atttctataa
    11161 ccactccagc cagtggcgtt atgaaaccgt cacggcggaa gagttgctgt caccgatggc
    11221 ggacaaatcc cgctataccg gacacttgat cgactttaac gtccgtgcgg aacgcatggg
    11281 ctggctgccg tctgcaccgc agttaggcac taacccgctg actatcgctg gcgaagcgga
    11341 aaaagccggg atgaatccgg tggactatac ggtgaaatcc ctgaaagagg gttccatccg
    11401 ttttgcggca gaacaaccag aaaacggtaa aaaccacccg cgcaacctgt tcatctggcg
    11461 ttctaacctg ctcggttctt ccggtaaagg tcatgagttt atgctcaagt acctgctggg
    11521 gacggagcac ggtatccagg gtaaagatct ggggcaacag ggcggcgtga agccggaaga
    11581 agtggactgg caggacaatg gtctggaagg caagctggat ctggtggtta cgctggactt
    11641 ccgtctgtcg agcacctgtc tctattccga catcattttg ccgacggcga cctggtacga
    11701 aaaagacgac atgaatactt cggatatgca tccgtttatt cacccgctgt ctgcggcggt
    11761 cgatccggcc tgggaagcga aaagcgactg ggaaatctac aaagccatcg cgaagaaatt
    11821 ctccgaagtg tgcgtcggcc atctgggtaa agaaaccgac atcgtcacgc tgcctatcca
    11881 gcatgactct gccgctgaac tggcgcagcc gctggatgtg aaagactgga aaaaaggcga
    11941 gtgcgacctg atcccaggta aaaccgcgcc acacattatg gtcgtagagc gcgcttatcc
    12001 ggcgacttac gaacgcttta cctctatcgg cccgctgatg gagaaaatcg gtaatggcgg
    12061 taaagggatt gcctggaaca cccagagcga gatggatctg ctgcgtaagc tcaactacac
    12121 caaagcggaa ggtccggcga aaggccagcc gatgctgaac accgcaattg atgcggcaga
    12181 gatgatcctg acactggcac cggaaaccaa cggtcaggta gccgtgaaag cctgggctgc
    12241 cctgagcgaa tttaccggtc gtgaccatac gcatctggcg ctgaataaag aagacgagaa
    12301 gatccgcttc cgcgatattc aggcacagcc gcgcaaaatt atctccagcc cgacctggtc
    12361 tggtctggaa gatgaacacg tttcttacaa cgccggttac accaacgttc acgagctgat
    12421 cccatggcgt acgctctctg gtcgtcagca actgtatcag gatcaccagt ggatgcgtga
    12481 tttcggtgaa agcctgctgg tttatcgtcc gccgatcgac acccgttcgg tgaaagaagt
    12541 gataggccag aaatccaacg gcaacccgga aaaagcgctc aacttcctga cgccgcacca
    12601 gaagtggggg atccactcca cctacagcga caaccagttg atgctgacct taggtcgcgg
    12661 tggcccggtg gtctggctga gcgaagccga tgccaaagag ctgggcattg ccgataacga
    12721 ctggattgaa gtttacaaca gcaacggtgc gctgactgcc cgtgcggttg tcagccagcg
    12781 tgttccggca gggatgacca tgatgtacca cgcgcaggaa cgtatcgtta acctgccggg
    12841 ttcggaaatt actggccagc gtggggggat ccacaactcg gtcacccgta tcacgccgaa
    12901 accgacgcat atgattggtg gctatgccca tctggcatac ggctttaact actacggcac
    12961 cgtaggttct aaccgcgatg agtttgttgt agtgcgtaag atgaagaaca ttgactggtt
    13021 agatggcgaa ggcaatgact aggtacagga gagcgtaaaa tgaaaattcg ttcacaagtc
    13081 ggcatggtgc tgaatctcga taagtgcatc ggctgccaca cctgttcagt tacctgtaaa
    13141 aacgtctgga ccagccgtga aggcgtggag tacgcgtggt tcaacaacgt ggaaaccaag
    13201 ccgggtcagg gcttcccgac tgactgggaa aaccaggaaa aatacaaagg cggctggatc
    13261 cgtaaaatca acggcaaact gcagccgcgc atgggtaacc gtgccatgtt gctgggtaaa
    13321 atcttcgcta acccgcatct gccggggatc gacgattatt acgagccgtt cgattttgac
    13381 tatcagaacc tgcataccgc gccggaaggc agcaaatcgc agccgattgc ccgtccccgt
    13441 tcgctgatta ccggggaacg gatggcgaaa atcgaaaaag ggccgaactg ggaagatgac
    13501 ctgggtggtg agtttgacaa actggcgaaa gacaagaact tcgacaacat ccagaaggcg
    13561 atgtatagcc agttcgaaaa cacctttatg atgtatttgc cgcgcctgtg cgaacactgc
    13621 ctgaacccgg catgtgtggc gacctgcccg agcggtgcga tttacaagcg tgaagaagat
    13681 ggcatcgtcc tgatcgacca ggataaatgc cgtggctggc gtatgtgcat caccggatgc
    13741 ccgtacaaaa aaatctactt caactggaag agcggtaagt ctgagaagtg catcttctgc
    13801 tatccgcgta ttgaagcggg tcagccgacc gtgtgctcag aaacctgtgt cggtcgtatc
    13861 cgttatcttg gcgtgctgct gtacgatgcc gacgcgattg aacgtgcagc cagcaccgag
    13921 aacgagaaag atctttacca gcgtcagctg gaggtgttcc tcgatccgaa cgatccgaaa
    13981 gtcatcgagc aggcgattaa agacggtatt ccgctgagcg ttattgaagc cgcacagcag
    14041 tcgccggttt ataaaatggc aatggaatgg aaactggcgc tgccgctgca tccagaatat
    14101 cgcacactgc cgatggtctg gtacgtgccg cctctgtctc cgattcagtc tgcagcagac
    14161 gcgggtgagc tgggtagcaa cggcattctg ccagacgtcg aaagcttgcg tattccggta
    14221 cagtatctgg cgaatctgct gaccgccggt gataccaaac cggtactgcg cgcactgaaa
    14281 cgtatgctgg cgatgcgtca ttacaaacgt gctgaaaccg ttgacggtaa agttgatacc
    14341 cgtgcgctgg aagaggtcgg tctgaccgaa gcccaggcac aggagatgta ccgttatctg
    14401 gctattgcta actacgaaga tcgctttgtg gtgccgagta gtcatcgtga actggcacgg
    14461 gaagccttcc cggagaaaaa tggctgcggc tttacctttg gtgatggctg ccacggctca
    14521 gataccaaat tcaatctgtt caacagccgt cgtatcgatg ccatcgatgt gaccagcaaa
    14581 acggagccgc atccatgatc gaactcgtga ttgtatcgcg tctccttgaa tatccggatg
    14641 ctgccttatg gcagcatcaa caagagatgt ttgaggtgat tgctgcgtcg aaaaatctgt
    14701 cgaaagaaga tgcccatgcg ctgggcattt tcctgcgcga tttaacggcg atggatccgc
    14761 tcgatgccca ggcccagtac agcgaactgt tcgaccgtgg ccgcgccacg tcactgttgc
    14821 tatttgaaca tgttcacggt gaatcccgtg accgcggcca ggcgatggtg gacctgctgg
    14881 cgcagtacga gcagcacggc ttgcagttaa acagccgcga attaccggac catctgccgc
    14941 tgtatctgga gtacctgtcg cagctgccgc aaagcgaagc cgtggaaggt ttgaaagata
    15001 tcgcgccgat tctggcattg ctgagcgcgc gtctgcaaca gcgtgaaagc cgttatgccg
    15061 tgatgtttga tctgctgctg aaattggcca ataccgctat cgacagcgac aaagtggcgg
    15121 aaaaaattgc cgacgaagcg cgcgatgata cgccgcaggc gctggatgct gtctgggaag
    15181 aagagcaggt taaattcttt gctgacaaag gctgcggtga ttcagcaatc actgctcatc
    15241 agcgtcgctt tgccggtgcc gtcgcgccgc aatatctgaa tatcaccacc ggaggacagc
    15301 actaatgcaa ttcctgaata tgttcttctt tgatatctac ccgtacatag ccggggcggt
    15361 cttcctgatt ggtagctggc tgcgttatga ctacgggcag tacacctggc gcgcggcgtc
    15421 cagccagatg ctggatcgca aagggatgaa cctggcgtcg aacctgttcc atatcggtat
    15481 tctggggatt tttgctggtc acttcttcgg tatgctgacg ccgcactgga tgtatgaagc
    15541 ctggctgccg attgaagtga aacagaaaat ggcgatgttt gctggtggtg ccagcggcgt
    15601 gctgtgtctg attggcggcg tgctgttgct gaagcgtcgt ctgttcagcc cacgcgtgcg
    15661 tgcaaccact accggagcgg atatcctgat cctgtcgctg ctcgttatcc agtgcgcgct
    15721 gggcctgttg accattccgt tctccgctca gcatatggac ggtagcgaga tgatgaaact
    15781 ggttggctgg gcgcagtcgg tggtgacctt ccacggtggc gcttctcaac acctcgatgg
    15841 tgtggcgttt atcttccgtc tgcacctggt gctggggatg acgttattcc tgctgttccc
    15901 gttctcgcgt ctggtacaca tctggagcgt accggtggag tatctgacac gtaagtacca
    15961 gctggtgcgc gctcgtcact aaacgaattt tagttcacat agaccctgct tcggcggggt
    16021 tttttatggg cacggtgcgg ggtgagttgt cggatgcgct tcgcttatcc gacctacagg
    16081 ggaggatatt gtaggcccgg taaacgtggt gccatcgggc aaaattagct cggagtaaca
    16141 ggttttgatt attcaaggag gcgatggtgg tgggggaagg attactcagc gctgcgcgct
    16201 tcgcccttcg ggtcgttgcc tgcggcaacg ctctctcgct ggcgctcaag tcgaaccttg
    16261 gtcgaagctt ctcatccttc cccgcttggg cagaatattt aattgcggat tcgtttgaga
    16321 gtttacggac ttttgaaaag tgatggtggt gggggaagga ttcgaacctt cgaagtcgat
    16381 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccag gggtaattca
    16441 aattttgagg taatgcttga gatggtggtg ggggaaggat tcgaaccttc gaagtcgatg
    16501 acggcagatt tacagtctgc tccctttggc cgctcgggaa ccccaccacg gggtaatgct
    16561 ttttactggc ctgctccctt atcgggaagc ggggcgcatc atatcaaatg acgcgccgct
    16621 gtaaagtgtt acgttgagaa aaatgaactg gttgcgtaat tttcatccgt aacggattaa
    16681 aggtaaccag ttatttttgc tggcgattaa agaataatcg tccgattacc gtaaacaaag
    16741 acgcgctgtg ccagtacttt gtatagcgca cgacttaaga cgtttttctc gacgtcacga
    16801 cctgcgcgca tcatatcttc agctgtgtag gtatgatcga catgaataac gtcctgcatg
    16861 atgattgggc cttcgtccag attgtcattc acatagtgag cggttgcgcc aataatcttc
    16921 acaccacgtt cataggcctg gtgataagga cgtgcgccga taaacgcagg caggaatgaa
    16981 tggtgaatat tgatgatttt attcgggaag cgtgccacaa attccggcgt taatacccgc
    17041 atatacttcg ccagcaccac atagtcaggt ttataagcat caatggcgtc tgccatcttt
    17101 tgatcgtgct cgttgcggct taacccttca tggtttacca gctcaaacgg aatatcaaaa
    17161 cgctcaacca gagaacgtaa agtatcgtgg ttaccaataa ctgccgcgat ttcgacatcc
    17221 aggccgccgt aattggcttt catcaacaaa tcgccaaggc aatgcgcttc tttagtgacc
    17281 agaatcacta tccggcgacg accggcagga ttcagctcac gcacggagcc ttctggcaat
    17341 gcgctatcga gatccgccag cagggtggaa tcattaaaaa tcccttccag ttccgtgcgc
    17401 ataaaaaagc gcccggtacg gtgatcaaca aattcattgt tctgtacgat atttaactcg
    17461 tgcttgtagc aaatattggt aatacgtgcg atcagacctt tttggtccgg acaaatagta
    17521 cgcagaactt tacgttggag tgaatgcatt gctggaaaaa ccttgttgag agtgtttgct
    17581 aaaccgtacc gtcaaccatt attggccgca gcacttttta aattttttac ctgaaccaca
    17641 agggcaggga tcgttgcgac caaactgcgg acgtgtaccg tcaatatagt accattgacc
    17701 gttttctttc aaaaatcgcg aacgttcaat tattgcacct gttttgccac cttcggtaaa
    17761 tcgagcaaca aaactgacaa atccgatatt gtcggcgtcc tgccaacaat gttcaaatac
    17821 ggtcaatccc aaccattcag tatgcgcaaa tccggccatc aattcggcac gtaacgccgc
    17881 tgctccacaa gagggatgcc aggtctttat taaataatct gcgtcttgca tcacaaaagc
    17941 gcagtaacgc gaacgcatga gatgttctgg atcaggtgca accttttcac cagacacata
    18001 agggtggcaa cataggctat actcgacagc actaccacag ggacaaagct gagacacaaa
    18061 taatctccct ggaaacaata acggcgtatt aaccgcctga gtagcactat gttaaccgag
    18121 cagtagcgat gtggctacga ttgcattcca ggggaatctt gcgggaataa tgagaaagat
    18181 aaaaataggg ctggcgctgg gatctggcgc ggcgagaggt tggtcgcata ttggcgttat
    18241 taatgcgcta aaaaaagtgg gtattgaaat tgatatcgtt gcaggatgtt caattggttc
    18301 gctggtgggc gctgcctatg catgcgatcg attatctgcg ctggaagatt gggtgacctc
    18361 tttcagttat tgggatgttt tacgcctgat ggatctctcc tggcagcgcg gtgggttact
    18421 gcgcggcgag cgtgtcttca atcaatgccg cgaaataatg ccggaaacag agatcgaaaa
    18481 ttgttcccgt cgctttgcgg ctgttgccac caatttaagt acgggacgtg aattatggtt
    18541 tactgaaggc gatctccatc ttgctattcg cgcatcatgc agtattccag gactcatggc
    18601 acctgttgca cataacggct actggctggt tgatggagca gtagttaacc caattcctat
    18661 ttccctcacg cgtgtattgg gggctgatat tgtgatagcg gtcgacctgc agcacgatgc
    18721 tcatttgata caacaagatt tgctctcctt taatgtcagt gaagaaaata gcgagaatgg
    18781 tgaatctttg ccgtggcatg cgcgtctgaa agaaaggtta ggcagtataa caacacgtcg
    18841 ggcggtgaca gcgccaacgg caacagagat tatgaccact tctatccagg tgctggagaa
    18901 ccgccttaaa aggaaccgca tggcaggtga tccgcccgat attctgattc aacctgtttg
    18961 cccgcaaata tctacgcttg atttccatcg cgcgcacgct gccattgcgg ccggacagct
    19021 ggcagtggaa aggaaaatgg acgaactttt gccgttggta cgcaccaaca tttgaccaga
    19081 atttttatct acacttaagt taattctgac aggcgcaggt ggcaatagca tgccactatt
    19141 gagtaaagcc agtcagggga gagaacatga cgcagccatt ggtcggaaaa cagattctca
    19201 ttgttgaaga tgagcaggta tttcgctcgc ttctggattc atggttttcc tcattgggag
    19261 cgacaacggt actggcggct gatggggtgg atgcccttga gttgctggga ggtttcactc
    19321 cagacctgat gatatgtgat atcgcgatgc cacgaatgaa cgggcttaaa ctgctggagc
    19381 atatacgtaa cagaggcgac cagaccccag ttctggtgat atctgccact gaaaatatgg
    19441 cagatattgc caaagcgtta cgtctgggcg ttgaagatgt tttgctgaaa ccagttaaag
    19501 atctgaatcg cttgcgcgag atggtttttg cctgtctcta tcccagcatg tttaattcgc
    19561 gcgttgagga agaggaaagg ctttttcgcg attgggatgc aacggttgat aaccctgccg
    19621 cagcggcgaa attattacag gaactacaac cgccggttca gcaggtgatt tcccattgcc
    19681 gggttaatta tcgtcaattg gttgccgcgg acaaacccgg cctggtgctt gatattgccg
    19741 cactttcgga aaacgatctg gcattttatt gccttgatgt cacccgagct ggacataatg
    19801 gcgtacttgc tgccttgtta ttacgcgcat tgtttaacgg attattacag gaacagcttg
    19861 cacaccaaaa tcaacggttg ccagagctgg gcgcgttatt gaagcaggta aaccatttac
    19921 tccgtcaggc caatctgccg gggcagtttc cgctattagt tggctattat catcgcgaac
    19981 tgaaaaatct cattctggtt tctgcgggtc tgaatgcgac gttaaatacc ggcgaacacc
    20041 aggtgcaaat cagtaatggt gttccgttag gcactttagg taacgcttat ttgaatcaat
    20101 tgagccagcg atgcgatgcc tggcaatgcc aaatatgggg aaccggtggt cgactgcgct
    20161 tgatgttgtc tgcagaatga gcaaacgata acgcggacta aatttgcatt acctgctaat
    20221 gtcggctggt ggtactatcg tcgccattcg tataagtaat tgtcttaatt atgctaactc
    20281 gcctcctttt cagaacttag ccccttcggg gtgctgatat actgggatgc gatacagaaa
    20341 tatgaacacg ttcaaaacac gaacagtcca ggagaattta aatggctgcc attaatacga
    20401 aagtcaaaaa agccgttatc cccgttgcgg gattaggaac caggatgttg ccggcgacga
    20461 aagccatccc gaaagagatg ctgccacttg tcgataagcc attaattcaa tacgtcgtga
    20521 atgaatgtat tgcggctggc attactgaaa ttgtgctggt tacgcactca tctaaaaact
    20581 cgattgaaaa ccactttgat accagttttg aactggaagc aatgctggaa aaacgtgtaa
    20641 aacgtcaact gcttgatgaa gtgcagtcta tttgtccgcc gcacgtgact attatgcaag
    20701 ttcgtcaggg gctggcgaaa ggcctgggac acgcggtatt gtgtgctcac ccggtagtgg
    20761 gtgatgaacc ggtagctgtt attttgcctg atgttattct ggatgaatat gaatccgatt
    20821 tgtcacagga taacctggca gagatgatcc gccgctttga tgaaacgggt catagccaga
    20881 tcatggttga acaggttgct gatgtgaccg catatggcgt tgtggattgc aaaggcgttg
    20941 aattagcgcc gggtgaaagt gtaccgatgg ttggtgtggt agaaaaaccg aaagcggatg
    21001 ttgcgccgtc taatctcgct attgtgggtc gttacgtact tagcgcggat atttggccgt
    21061 tgctggcaaa aacccctccg ggagctggtg atgaaattca gctcaccgac gcaattgata
    21121 tgctgatcga aaaagaaacg gtggaagcct atcatatgaa agggaagagc catgactgcg
    21181 gtaataaatt aggttacatg caggccttcg ttgaatacgg tattcgtcat aacacccttg
    21241 gcacggaatt taaagcctgg cttgaagaag agatgggcat taagaagtaa catccatatc
    21301 ggtgttatcc acgaaacggc gttgaacaat cgacgccgtt tttttatagc ttattcttat
    21361 taaattgtct taaaccggac aataaaaaat cccgccgatg gcgggatttt tagcaattgc
    21421 aatctacaaa agattattgc ttgatcagga aatcgtcgag ggatttacct tgctcatcca
    21481 ttgctttttt gattacagct ggagtacggc cctggccagt ccaggtttta gtttcgccgt
    21541 tttcgtcaac atagctatat tttgccggac gctgagcacg tttagctttg gtgccggatt
    21601 taacggcagc aaggctattc agcagttcgt tcgggtcaat accgtcagcg atcagcattt
    21661 cgcgatattg ctgcagttta cgagtgcgct cttcaacttc agcagcagcc gcgctttctt
    21721 cttcgcgacg ttcgttaaca acaacttcta atttttccag catttcttcc agcgtttcaa
    21781 gtgtacattc tcttgcctgc gcacgaagag tacggatgtt gttcagaatt ttaagtgctt
    21841 cgctcattgt agtaatctca aacttatatt ggggtggttt gttgaggtaa taatagagcc
    21901 ttaaattcag ttgtgcaata gccaggaatg taaggaattc aaaattgttc tttattttgt
    21961 gccgccaata aatatctttt cataaaatta gccagaaaag acgcggcata tagccctatt
    22021 tacaccgatg atttcgcagc acgtgaggtt aaaacttcct gattcatgtc acattttatg
    22081 gggagattat cgtaggctga cggcctttca gtcttctgta ttagttgtgt ttacgagaat
    22141 tccctattaa gcgaatgatg aaaagtagaa cagtcgcaat aagagcatgg acttagtatt
    22201 gcactatctc ctggaggtca acagagggct attacttgcg caacaggtta aagattgtga
    22261 atagttacca gcagtcattt acccgcttat aacaagcgag gcagttgtaa tgatagctca
    22321 gaaggattat gcaaggcttc gtaagggaga acgcatatac ccacttctgt gcatactgtt
    22381 gagctgaaaa actgacgaat tatgataaac tccagccaac tttatttcat atcattgagg
    22441 gcctgtggct gatggcacag ctatatttct actattccgc aatgaatgcg ggtaagtcta
    22501 cagcattgtt gcaatcttca tacaattacc aagaacgcgg catgcgcact gtcgtatata
    22561 cggcagaaat tgatgatcgc tttggtgccg ggaaagtcag ttcgcgtata ggtttgtcat
    22621 cgcctgcaaa attatttaac caaaattcat cattatttga tgagattcgt gcggaacatg
    22681 aacagcaggc aattcattgc gtactggttg atgaatgcca gtttttaacc agacaacaag
    22741 tatatgaatt atcggaggtt gtcgatcaac tcgatatacc cgtactttgt tatggtttac
    22801 gtaccgattt tcgaggtgaa ttatttattg gcagccaata cttactggca tggtccgaca
    22861 aactggttga attaaaaacc atctgttttt gtggccgtaa agcaagcatg gtgctgcgtc
    22921 ttgatcaagc aggcagacct tataacgaag gtgagcaggt ggtaattggt ggtaatgaac
    22981 gatacgtttc tgtatgccgt aaacactata aagaggcgtt acaagtcggc tcattaacgg
    23041 ctattcagga aaggcatcgc cacgattaat aagaatttct ttactgacag ggtgagcagg
    23101 gcacttttat cctgtcagtt cgttttacgc acttcttccg ggctatatac ccttctcggc
    23161 agttttttaa cgccgctata cgcctcacag ggctcttaag caccgacgtt gacttgtgac
    23221 ctgtaaagta caatatccct gtgtttaggc gttatacatc gtcgcaaata tgatgaaggc
    23281 taatgctgtc ggtttatgga aaagttgctt tgggtaaaca aaaaatacgg ccccagaagg
    23341 gcaatgccgt tcacttaaga ggagcggcac tatgtttcac aggataacgg gtttttgata
    23401 tcttaaccga cctcggcctt gatggtcggg ggcgttttgt tatgaacacc acttccagag
    23461 caccccgaag atgctccagt cgtttcggga tggtccctgg tgacgccgtg tttcccagct
    23521 ctatcatttc tgatgcgata ttcttccacg caggcagtag ccagtggcgg ttacaaccct
    23581 tctggttcag cgttagcagc aggtcttcgc tgtaaaaaag cttatcaaac aacgtaatag
    23641 agttatccgg gatggtggcg agcatggagt gggccagcac agtttcgctc tgccggtaag
    23701 gtgcggtcac ggcattcagc agaatgtgac ttcccaggtt cattaaggcc accagacgca
    23761 taaccgggta ggcgttctgc cgcttagtgg atgtgttggc agacccataa tattcacgca
    23821 gctcgggttt atcaggtgtc ctgaactgtg cgccatcaat ggcaaaaagt tgcaggccgt
    23881 gccagtcatc cttcaggtaa cgttccgcgc ccctgtctgt gcggtctggc ggaagagcca
    23941 ttccactggg gcggccccca cgcgctgacg cgcttgggtg acagcgctgc gggccagcag
    24001 gttcatcccc gcttcgccat ccgcgctcag gttcagacgg cgaacaacat cggtaattgg
    24061 ctcattgcac caccatccag ataaccatgt cccccggtaa acgacggcgg cgaacggtcg
    24121 catgagcaga aagcgtcagg cagtgttgta tccactcggt gggaaggtgt tctgcaaata
    24181 gttgtgcaga gggcggaggc ataagcggat ggtcactgaa atcgagcaga tcattgagaa
    24241 gtggcataag aaaacggctc cctattgtgg aagccgttat agtgcctcag tttaaggatc
    24301 ggtcaactaa tccttaactg atcggcattg cccagaaggg gccgtttatg ttgccagaca
    24361 gcgctactga ttaagcggat tttttcgctt ttttctcagc tttagccgga gcagcttctt
    24421 tcttcgctgc agtttcacct tctacataat cacgaccgta gtaggtatcc agcagaatct
    24481 gtttcagctc ggagatcagc gggtaacgcg ggttagcgcc ggtgcactgg tcatcgaatg
    24541 catcttcaga cagtttatcc acgttcgcca ggaagtctgc ttcctgaacg ccagcttcac
    24601 ggatagattt cggaataccc agttcagctt tcagcgtttc cagccatgcc agcagtttct
    24661 cgatcttagc agcagtacgg tcgcccggtg cgctcagacc caggtggtca gcaatttcag
    24721 cataacgacg gcgagcctgc ggacggtcat actggctgaa tgcagtctgc ttggtcgggt
    24781 tgtcgttcgc gttgtagcga ataacgttac aaatcagcag ggcgtttgcc agaccgtgcg
    24841 gaatatggaa ctgggaaccc agtttgtgcg ccattgagtg acatacaccc aggaaggcgt
    24901 tcgcaaacgc gatacccgcg atagtcgctg cactgtgaac acgttcacgc gctaccggat
    24961 ttttagaccc ttcgtggtag gacgctggca gatattcttt cagcagtttc agtgcctgca
    25021 gagcctgacc atcagagaac tcagatgcca gtacagaaac ataagcttcc atggcgtgag
    25081 ttactgcgtc cagaccaccg aaagcacaca gggacttcgg catgtccata accaggttgg
    25141 cgtcgacaat cgccatatcc ggagtcagcg catagtctgc cagcggatat ttctgaccag
    25201 tagcgtcgtc agttacaacc gcaaacggag tgacttcaga acctgtacca gaagtggtgg
    25261 tgacagcgat cattttcgct ttcacgccca ttttcgggaa cttgtagata cgtttacgga
    25321 tatccataaa gcgcagcgcc agctcttcga agtgagtttc cggatgttcg tacataaccc
    25381 acatgatctt cgcggcgtcc atcggggaac caccacccag cgcgataatc acgtctggtt
    25441 tgaaggagtt tgccagttct gcacctttac gaacgatgct cagggtcggg tccgcttcta
    25501 cttcgaagaa gacttcagtt tcaacgcctg ctgctttcag tacggaagtg atctgatcag
    25561 cataaccatt gttgaacagg aagcggtcag tcacgatgag cgcacgtttg tggccatcag
    25621 taatcacttc atccagcgcg attggcaggg agccacggcg gaagtagata gatttcggaa
    25681 gtttgtgcca caacatgttt tcagctcgct tagcaacggt tttcttgttg atcaggtgtt
    25741 tcggaccaac gttttcagag atggagttac caccccaaga accacaaccc agagtcaggg
    25801 aaggtgcgag tttgaagtta tacaggtcac cgataccacc ctgagacgct ggggtgttaa
    25861 tcaggatacg agccgttttc attttctgac cgaagtaaga aacgcgagcc ggttggttat
    25921 cctggtcagt gtacaggcaa gaggtatgac cgataccgcc catagcaacc agtttctctg
    25981 ctttttctac cgcgtcttcg aaatctttag cgcggtacat tgccagagtc ggggacagtt
    26041 tttcatgtgc gaacggttcg ctttcatcaa caacggtcac ttcaccgatc agaatcttgg
    26101 tgttttctgg tacagagaag cctgccagtt cagcaatttt ataggctggc tgaccaacga
    26161 tagccgcgtt cagcgcaccg tttttcagga taacatcctg aacagctttc agctctttac
    26221 cctgcaacag atagccgccg tgggttgcaa aacgttcacg tacagcgtca taaacagagt
    26281 caacaacaac aacagactgt tcagaagcac agattacgcc gttgtcgaag gttttggaca
    26341 tcagtacaga tgcaactgca cgtttgatat cagcagtttc atcgataaca actggagtgt
    26401 tgcccgcgcc tacaccgata gctggtttac cggagctgta tgcggcttta accatgcccg
    26461 gaccaccagt cgcgaggatc aggttgatgt ctgggtggtg catcagtgcg ttagacagtt
    26521 caacagaagg ttgatcgatc cagccgatca gatctttcgg agcaccggca gcgatagcag
    26581 cctgcagaac gatatcagcc gctttgttgg tggcatcttt tgcacgcggg tgcggggaga
    26641 agataatggc gttacgggtc ttcagactga tcagcgattt gaagatagca gttgaagtcg
    26701 ggttagtggt cggaacgata ccgcaaataa taccgattgg ttcagcgata gtgatggtac
    26761 caaaagtgtc gtcttcagac agaataccac aggttttttc atctttatag gcgttgtaga
    26821 tatattcaga agcaaagtgg tttttgatca ctttatcttc gacgataccc atgccggatt
    26881 cggcaacggc cattttcgcg agtgggattc gagcatctgc agcagccaga gcggcggcgc
    26941 ggaagatttt gtctacttgc tcttgagtga aactggcata ttcacgctgg gctttcttta
    27001 cacgctctac gagtgcgtta agttcagcga cattagtaac agccataatg ctctcctgat
    27061 aatgttaaac ttttttagta aatcatctgc tcgaatacga gagtatagtc agtgcggtga
    27121 tgatttgctt aacctatgaa aatcaaaagc ttactcgcgc tcacactcac tgtgatttac
    27181 taaaagagtt taaacattag agttattatc tctaatgcgt cacttccagg tggcgtaagc
    27241 aagattactc acttctgggt actgattacg tgatccaaat caaatttttt gcaaagctga
    27301 cacctttcag catcgctttt cgccattata gctaacagtt aataaattgt agtatgattt
    27361 ggtggctaca ttagcatgtt ttgcacaact agataacaat aacgaatgat agcaatttta
    27421 agtagttagg aggtgaaaaa tgctgtcaaa aggcgtattg tcagcgcgtc ttttcaacct
    27481 tatttatggc taacattatc cggcttttgc ttcggagcta accgtgattc agaccttttt
    27541 tgattttccc gtttacttca aatttttcat cgggttattt gcgctggtca acccggtagg
    27601 gattattccc gtctttatca gcatgaccag ttatcagaca gcggcagcgc gaaacaaaac
    27661 taaccttaca gccaacctgt ctgtggccat tatcttgtgg atctcgcttt ttctcggcga
    27721 cacgattctg caactttttg gtatatcaat tgattcgttc cgtatcgccg ggggcatcct
    27781 ggtggtgacg atagcgatgt cgatgatcag cggcaagctt ggcgaggata aacagaacaa
    27841 gcaagaaaaa tcagaaaccg cggtacgtga aagcattggt gtggtgccat tggcgttgcc
    27901 gttgatggcg gggccagggg cgatcagttc taccatcgtc tggggtacgc gttatcacag
    27961 cattagctat ctgtttggtt tctttgtggc tattgca
//
LOCUS       DAJTNN010000035        27775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTNN010000035 DAJTNN010000000
VERSION     DAJTNN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.035"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..542)
                     /locus_tag="OO150_001355"
                     /pseudo
     CDS             complement(1..542)
                     /locus_tag="OO150_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
     gene            complement(720..1631)
                     /gene="gsiD"
                     /locus_tag="OO150_001356"
     CDS             complement(720..1631)
                     /gene="gsiD"
                     /locus_tag="OO150_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5515553.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1634..2554)
                     /gene="gsiC"
                     /locus_tag="OO150_001357"
     CDS             complement(1634..2554)
                     /gene="gsiC"
                     /locus_tag="OO150_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5515554.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2572..4110)
                     /gene="gsiB"
                     /locus_tag="OO150_001358"
     CDS             complement(2572..4110)
                     /gene="gsiB"
                     /locus_tag="OO150_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5515555.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILVH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4130..6001)
                     /gene="gsiA"
                     /locus_tag="OO150_001359"
     CDS             complement(4130..6001)
                     /gene="gsiA"
                     /locus_tag="OO150_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5515556.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLCVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5988..6953)
                     /gene="iaaA"
                     /locus_tag="OO150_001360"
     CDS             complement(5988..6953)
                     /gene="iaaA"
                     /locus_tag="OO150_001360"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5515557.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPAIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIGHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7157..8392
                     /gene="moeA"
                     /locus_tag="OO150_001361"
     CDS             7157..8392
                     /gene="moeA"
                     /locus_tag="OO150_001361"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5515558.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8392..9141
                     /gene="moeB"
                     /locus_tag="OO150_001362"
     CDS             8392..9141
                     /gene="moeB"
                     /locus_tag="OO150_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5515559.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHNDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEYDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9217..9879)
                     /gene="fsa"
                     /locus_tag="OO150_001363"
     CDS             complement(9217..9879)
                     /gene="fsa"
                     /locus_tag="OO150_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5515560.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            10010..10909
                     /locus_tag="OO150_001364"
     CDS             10010..10909
                     /locus_tag="OO150_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5515561.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            10915..13347
                     /locus_tag="OO150_001365"
     CDS             10915..13347
                     /locus_tag="OO150_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5515562.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13493..14308
                     /gene="ybiV"
                     /locus_tag="OO150_001366"
     CDS             13493..14308
                     /gene="ybiV"
                     /locus_tag="OO150_001366"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5515563.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14460..15725
                     /locus_tag="OO150_001367"
     CDS             14460..15725
                     /locus_tag="OO150_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5515564.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFSREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSTSRPEIYPIYWSQTQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     HEDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15966..17558)
                     /locus_tag="OO150_001368"
     CDS             complement(15966..17558)
                     /locus_tag="OO150_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5515565.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17777..18697
                     /gene="ldtB"
                     /locus_tag="OO150_001369"
     CDS             17777..18697
                     /gene="ldtB"
                     /locus_tag="OO150_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5515566.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18756..19874)
                     /locus_tag="OO150_001370"
     CDS             complement(18756..19874)
                     /locus_tag="OO150_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5515567.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(19871..20338)
                     /gene="mntR"
                     /locus_tag="OO150_001371"
     CDS             complement(19871..20338)
                     /gene="mntR"
                     /locus_tag="OO150_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5515568.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20524..20652
                     /gene="mntS"
                     /locus_tag="OO150_001372"
     CDS             20524..20652
                     /gene="mntS"
                     /locus_tag="OO150_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5515569.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            20924..22507
                     /gene="opgE"
                     /locus_tag="OO150_001373"
     CDS             20924..22507
                     /gene="opgE"
                     /locus_tag="OO150_001373"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5515570.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22556..23071)
                     /gene="ompX"
                     /locus_tag="OO150_001374"
     CDS             complement(22556..23071)
                     /gene="ompX"
                     /locus_tag="OO150_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5515571.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23124..23189)
                     /locus_tag="OO150_001375"
     CDS             complement(23124..23189)
                     /locus_tag="OO150_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515572.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23424..24311
                     /gene="rhtA"
                     /locus_tag="OO150_001376"
     CDS             23424..24311
                     /gene="rhtA"
                     /locus_tag="OO150_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5515573.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24610..25113
                     /gene="dps"
                     /locus_tag="OO150_001377"
                     /gene_synonym="pexB"
     CDS             24610..25113
                     /gene="dps"
                     /locus_tag="OO150_001377"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5515574.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25517..26263
                     /gene="glnH"
                     /locus_tag="OO150_001378"
     CDS             25517..26263
                     /gene="glnH"
                     /locus_tag="OO150_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5515575.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26402..27061
                     /gene="glnP"
                     /locus_tag="OO150_001379"
     CDS             26402..27061
                     /gene="glnP"
                     /locus_tag="OO150_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5515576.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27058..>27775
                     /gene="glnQ"
                     /locus_tag="OO150_001380"
     CDS             27058..>27775
                     /gene="glnQ"
                     /locus_tag="OO150_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5515577.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHV"
BASE COUNT         6850 a         7431 c         6895 g         6599 t
ORIGIN      
        1 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
       61 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
      121 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttactta ttcctgatga
      181 taaacaacga ttttatcatt cgccctccgt gaaaagagat aatatttatt atcatcaagt
      241 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcacgttga
      301 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
      361 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
      421 tttaccgcgc gttccgatac cagttgatag ttaatgatga aaaaaagacc aataaataac
      481 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
      541 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
      601 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
      661 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
      721 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
      781 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
      841 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
      901 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
      961 accacgatag acgagacggt cccaggcaag atatgacgca ataaaatggt catatcgctg
     1021 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     1081 cgcaccagtc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     1141 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     1201 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     1261 aagcccagca acgtcccgat cgccgcaccg ataaacacgg caaacacgcc cgccgccagc
     1321 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     1381 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     1441 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     1501 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     1561 cgcacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     1621 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     1681 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     1741 ggtaatcgcg catttctacg gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     1801 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
     1861 ctaccgggat catcgcgtta cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
     1921 ctttcgccct cgcggtacgc atataatctt cgcttaaaac atcgacaaac gacgcgcggg
     1981 taaagcgcgc catcacggct gccactgccg cgccgagcgt cagggaggat aaaatgtagt
     2041 gctgccaact gtctgctccc acggtaggca gccagcccag ttcaacggag aatacctgaa
     2101 ttaaaagcat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
     2161 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
     2221 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
     2281 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
     2341 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
     2401 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
     2461 cgggcagcat atgcacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
     2521 accccagtaa gcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
     2581 atccgcgtct tcaaagctga agccggtgtc tggcatgatc caaaaaccgg tcaggttttt
     2641 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgact cttgccagat
     2701 gatatcctgc gccgccttat acaagcgggt cttttccgcc ggatcattag ttttcagtgc
     2761 ctgagccagg aagtcgtcca cctgtttatt gctgtaaaat gcggtattaa acagcgtcgg
     2821 tggccagttt tgcgaggcga acagtggcga tagcgcccag tccgcttcgc cggttgaagc
     2881 cgaccagcca gtgtagaaca tccgcacgcc gctctctttt tgccctttac cttcaacttc
     2941 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
     3001 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
     3061 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
     3121 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
     3181 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
     3241 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
     3301 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
     3361 tgcggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
     3421 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
     3481 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
     3541 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
     3601 cgctgccggt gaaatcatcg cggtcgccgg atggacaaga atattaataa acgctgagaa
     3661 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
     3721 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
     3781 tttcaccgcc gcggcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
     3841 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
     3901 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
     3961 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
     4021 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
     4081 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
     4141 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
     4201 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
     4261 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
     4321 gcgtataagg atgctgcggg ttttcgaaga ccgcgcgccg tggaccaatt tcaacaattt
     4381 gcccgagata catcaccgcc acacgatgac taatccgctc taccacggcc atatcgtggg
     4441 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
     4501 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
     4561 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
     4621 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
     4681 cagccgcgtc tttacctggc aataaaccgt gtacacacag cggttcgata atcgaatcac
     4741 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
     4801 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
     4861 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
     4921 tgccagactc gcccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
     4981 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
     5041 tgacaagatt acgcactcgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
     5101 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
     5161 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
     5221 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
     5281 ccagtacccg atcggctatc tctgccacca cgcccatatc gtgcgtgata aagataacgc
     5341 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
     5401 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
     5461 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcggataa cgtgaaagaa
     5521 tggtttgtgc ctcaggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
     5581 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
     5641 ataccgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgaa
     5701 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacttcgcga ctgcgccgct
     5761 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacagg cgcatcaatg
     5821 ccaacgcagt cactgactta ccggagccgg attcgccaac aattgccagc gtctcaccgc
     5881 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
     5941 caatattcag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
     6001 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
     6061 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gccgatagcg
     6121 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
     6181 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
     6241 cggatgaaga cttcgcccgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
     6301 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
     6361 cctgtggacg tggctgccgc caaattgccg tctaaatcca acgccacggc ccccacggtg
     6421 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggacggt tgccccttcc
     6481 ttgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatcgc cggcgagacg
     6541 cgctccatgc cacgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
     6601 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
     6661 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
     6721 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
     6781 cgcaccgctt ccgtcaccac atccagcgca ctttcgcccg cttccagcat tttctgcccg
     6841 gtttcaacaa tggcagacaa cgcctcaatg tagcgtaatt cctgttgcag actcatctgc
     6901 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
     6961 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
     7021 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
     7081 ccgaggtggc atacagtctg tggcgttttt ctgacataat aggcaaattc gattttgcct
     7141 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
     7201 tgagatgctt tctcgcgtca ccccactgac cgcccaggaa acgctgccac tggtacagtg
     7261 ttttggtcgt attctggcga gcgatgtcgt ttcgccgctt gatgttccgg ggtttgataa
     7321 ctccgcaatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
     7381 cgttgccggt aaatcctttg ccggtcagcc gtatcatggt gaatggcctg cgggcacctg
     7441 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
     7501 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
     7561 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
     7621 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
     7681 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
     7741 gctgggtgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
     7801 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccccatg ccctgcgcgc
     7861 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
     7921 gggtgaagcg gattacacca aaacgattct tgaagagctg ggggagatcg ccttctggaa
     7981 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
     8041 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
     8101 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
     8161 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
     8221 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
     8281 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
     8341 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
     8401 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
     8461 gacggtcagg aggcgctaaa agattctcgt gtgctgatcg tcggcctcgg tggactcggc
     8521 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
     8581 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acaatgatgc cacggtcggg
     8641 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
     8701 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaatacgat
     8761 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
     8821 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
     8881 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
     8941 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga tcggcgtaat tggttcgttg
     9001 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
     9061 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg taatccgggg
     9121 tgtgaggtgt gcgggcagta attgccacct ccaggccaga taagacgcgg cactccgcca
     9181 cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc tgccaaacgc
     9241 tcccagccag tcctgttcaa acttcgccac agcggcatca accgccggat agctaatcat
     9301 ctgttgtgcc acatccagtg gcagcgtaat tgattcacat cctgccagta agcagtccag
     9361 cgcctgacgc gggtttttga aactcgctgc cagcactttc gcctgcggcg catgcatttt
     9421 caataactgg tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag catcaatacg
     9481 attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcagcc cttgtgctgc
     9541 gccatatacc gcggttccca gcgttggaat cccttccgct tttaacatct taatagctgc
     9601 cagcccctcg gcggtcaccg gaactttcac cacgatatcc gcaataatag aacgcaattt
     9661 acgtgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa acagacgccc
     9721 ctgaccgccc atcgcttcat gaagttgcgg aagcacaaca tccagcggtt ttttacccgc
     9781 ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg acagcgcctt
     9841 cactgcaaca acgtctgaag tatccagata cagttccata accatcctca aaatgtactt
     9901 tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca catcaatcca
     9961 actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta tgattttcaa
    10021 tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat ttcttaaagg
    10081 ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgca cgcaggatct
    10141 gctgtatgac gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg cagcgccgga
    10201 agtgattgag cgcgcattga atggtttgct tattcatcgg gaaaagttaa ccccggagca
    10261 tctgacggcg ctaaccgact gctgcccgac acaggcgtta accgtgtgtg gggaagtgaa
    10321 aagcgttgag gagatcatgg cgaccgttct gcgcgataaa ccgttttacg atcgcagcgg
    10381 cggcggctta acgctttcgg gtggtgaacc ctttatgcag ccggaaatag cgacggcgct
    10441 actgcaagcc tgccacgagg caggcattca tactgcggta gaaacctgtc tgcatgtgcc
    10501 gtggaaatat atcacccctt ctctgcccta tatcgatctg tttcttgccg atttaaaaca
    10561 cattgccgac gcgccgttta aacagtggac cgacggtaac gccgccaagg tgctggataa
    10621 cctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc tgattcaggg
    10681 ctttaatgcc gacgaaacct ctgtaaaagc cattaccgat tttgccgccg acgagctgca
    10741 cgttggcgaa attcattttc tgccctacca cacgctgggc atcaacaaat atcacttact
    10801 taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagacctgc tcgactttgc
    10861 ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac aaccatgacc
    10921 acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc gctggtgcat
    10981 attgtgaaac cgccggtctg taccgagcgc gcgcagcact ataccgagat gtatcaacaa
    11041 catctcgata agccgatccc ggtacgtcgc gcgctggctc tggcgcatca cctggcgaat
    11101 cgcaccatct ggatcaagca cgatgagttg atcatcggca accaggcaag cgaggttcgc
    11161 gctgcgccga tcttcccgga atataccgtc tcgtggatcg aaaaagagat tgatgatctg
    11221 gcagatcgtc ccggtgctgg ttttgcggtg agcgaagaga acaaacgcgt tctgcatgaa
    11281 gtgtgcccgt ggtggcgcgg tcagacggta caggatcgct gctacggcat gtttaccgat
    11341 gagcaaaaag gtctgctggc gaccggaatc attaaagcgg aaggcaatat gacctccggc
    11401 gatgcgcacc tggcggtgaa tttcccgctg ctgctggaaa aaggtcttga tggtctgcgc
    11461 gagaaagtag cggaacgtcg ctcgcgcatc aatctgacgg tactggaaga tttgcacggc
    11521 gaacagttcc tgaaagcgat tgatatcgtg ctggtggcag tcagcgaaca cattgaacgt
    11581 tttgcagccc tggcgcgtga aatggccgcg accgaaaccc gcgaaagccg tcgcgatgaa
    11641 ctgctggcga tagcagaaaa ctgcgatctt atcgcccacc agccgccgca gactttctgg
    11701 caggcgctgc aactgtgtta cttcatccag ttgatattgc agatcgaatc taacggtcac
    11761 tcggtatcgt ttggtcgtat ggaccagtat ctctacccgt actatcgccg agacgttgaa
    11821 ctcaaccaga cactggatcg cgaacacgcc atcgaattgc tgcatagctg ctggctgaaa
    11881 ctgctggaag tgaacaagat ccgctccggc tcacactcaa aagcctctgc gggaagtcct
    11941 ctgtatcaga acgtcaccat tggcggacaa actctggttg atggtcaacc aatggacgcg
    12001 gtgaatccac tctcttacgc gatcctcgaa tcctgcggtc gcctgcgttc cactcagcct
    12061 aacctcagcg tgcgttacca tgcaggaatg agtaatgatt tcctcgacgc ctgcgtacag
    12121 gtgatccgtt gcggcttcgg gatgccggcg ttcaacaacg acgaaatcgt gatcccggaa
    12181 tttattaaac tcggtattga accgcaggac gcttacgact acgcagcgat tggttgtatc
    12241 gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt tatcaacttc
    12301 gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg ccacaagcgg caacgtgttc
    12361 ctgccacaag aaaaagcgtt gtcggcaggc aactttaaca atttcgatga agtaatggac
    12421 gcgtgggata cgcaaatccg ttattacacg cgtaaatcaa tcgaaatcga atatgtcgtc
    12481 gacaccatgc tggaagagaa cgtgcacgat attctctgct cggcgctggt ggatgactgt
    12541 attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt ttctggcctg
    12601 caggttggca ttgccaacct cggcaacagc ctggcggcag tgaagaaact ggtatttgaa
    12661 cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt cgatggcctg
    12721 actcatgagc aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg caacgacgat
    12781 gatactgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga actgaaacag
    12841 taccataatc cgcgctacgg tcgtggtccg gttggcggca actattacgc gggtacgtca
    12901 tctatctccg ctaacgtacc gtttggtgcg caaactatgg caacgccgga cgggcgtaaa
    12961 gcccacaccc cgctggcaga aggcgctagt ccggcctctg gtactgacca tctcggccct
    13021 actgcggtca ttggctcggt gggtaaactg cctacggcag cgattctcgg cggcgtgttg
    13081 ctcaaccaga aactgaatcc ggcaacgctg gagaacgaat ctgacaagca gaagctgatg
    13141 atcctgctgc gcaccttctt cgaggtgcat aaaggctggc atattcagta caacatcgtt
    13201 tcccgcgaaa cgctgctgga ggcgaaaaaa catccggatc agtaccgcga tctggtagtg
    13261 cgtgtcgcgg gttattccgc cttcttcacc gcgctctctc cagacgctca ggacgatatc
    13321 atcgctcgta ctgaacatat gctgtaatct cttcggcccg gctgcggcgt cgccgggcta
    13381 aatcattcac atcatcaatt tcatccttac tttcattcga aatataattt gtgctctgcg
    13441 tcacattgtt attacactgt tacggtttca ttttcgagcc aggagcacac ctatgagcgt
    13501 aaaagttatc gtcacagaca tggacggtac ttttcttaac gacgccaaaa cgtacaacca
    13561 accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattgagt tcgttgtcgc
    13621 cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg atgagatctc
    13681 ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcaactgt tccacggcga
    13741 actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata agcaactcaa
    13801 ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg aagcatttgt
    13861 cgcactgatg gcaaaacact accatcgcct gaaagctgta aaagattatc aggagattga
    13921 cgacgtactg ttcaagtttt cgctcaacct gccagatgaa caaatcccgt tagtgatcga
    13981 caaactgcac atagcgttcg atggcattat gaaacccgtc accagtggtt ttggctttat
    14041 cgacctgatt attcccggtc tacataaagc aaacggtatt tcgcggttac tgaaacgctg
    14101 ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg cggagatgct
    14161 gaaaatggcg cgttattcct ttgcgatggg caatgctgcg gaaaacatta aacaaatcgc
    14221 ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc aggcggtgct
    14281 ggataacaca tcccctttta acagctgacc tctctaccgg agccgtgcgc tccggttttc
    14341 ccttcttttt acatcctgta catcacatca aatcattaaa cttgcattaa cttatttata
    14401 acttatattg tcacatgcaa atcataatgg tttcgaatga aagttaatga gaggtggtta
    14461 tggcttctac ttttaccagc gacacattgc cagccgatca caaagcagct atccgtcaga
    14521 tgaagcacgc gctgcgggcg cagcttggcg acgtccagca gatctttaat cagctaagcg
    14581 atgacattgc cacgcgagtg gctgaaatca acgcactcaa agcacagggc gatgccgtct
    14641 ggccggtgct gtcttatgcc gatatcaaag caggtcatgt tactgcagag cagcgcgaac
    14701 agattaaacg tcgcggttgt gcggtgataa aaggccattt ctcccgcgaa caagcgctag
    14761 gctgggatca gtcgatgctg gactatctgg accgcaaccg ctttgacgag gtctacaaag
    14821 gccccggcga taatttcttc gggacgctca gcacttcacg tcccgagatt taccccatct
    14881 actggtcgca gacgcaaatg caggcccgcc agagtgaaga aatggcgaat gcgcagtcgt
    14941 ttctcaatcg tctgtggaca tttgaaagtg atggaaagca atggtttaac ccggatgtga
    15001 gcgtcatcta ccctgaccgt atccgccgcc gtccgcccgg aacgacctcc aaaggtcttg
    15061 gagcgcatac cgactccggg gcgctggaac gctggctgct tccagcgtat cagcgcgttt
    15121 tcgccaacgt ctttaatggc aatctggcgc aatatgatcc ctggcatgcg gcacatcgta
    15181 cggaagttga agagtacacg gtggacaaca ccaccaaatg ttccgtgttt cggacattcc
    15241 agggctggac agcgctctct gatatgctgc ctggtcaggg gttgctgcac gttgtgccca
    15301 ttcctgaagc catggcgtac gtactgttac gtccgctgct tgatgatgtg ccggaggatg
    15361 aactgtgcgg cgtagcgccc ggaagagtgt tgccggtatc agagcaatgg catccactgt
    15421 taattgaggc gttaaccagc attccaaaac tcgaggccgg agactccgtc tggtggcact
    15481 gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca gggttggggc aacgtgatgt
    15541 acattcctgc ggcaccgatg tgcgagaaaa atcttgccta cgcgcacaag gtgaaggccg
    15601 cactggaaaa aggcgcatcg ccgggcgact tcccgcacga ggactatgaa acaaactggg
    15661 aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg ggcatggatg
    15721 tttgagagtt aatggaagtg aattgatgtg ggattaagaa aatcgattcg acgttactca
    15781 gattgctgac aagtgcgctt tcttaatgcc tgatgcggcg ttaacgcctt atctggccta
    15841 caaagtcctg cagattcaat atattacagg tactgcgtag gcctgataag cgtagcgcat
    15901 caggctattt tgcatttgtc atcagccgcc gtcaccccga cagtatcggg gtgacggata
    15961 atcacttact cgatcccttt actacgcagg taatcttcgt aattaccgct aaagtcgatc
    16021 acgcgttccg gggtgatttc cagaatgcgg gtcgccaggg agcttacgaa ctcacggtcg
    16081 tgtgaaacaa agatcagcgt gccctgatac agttccagcg ccatgttcag tgactcgatg
    16141 gattccatat ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg cttctgcatc
    16201 attaacttac caaacagcat ccgccctttt tccccaccgg aaagcacttt agctggcttt
    16261 ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc tgcgtaccgc ctgctcgtca
    16321 tcgccttcct gcttccactg actcatccat tcgaacacgg tcagatcatt ttcaaactca
    16381 tattcgtggt cctgggcgta gtaaccaatg cgcgcgtttt cagaccattt tacggtgccg
    16441 ctgtccggtt gcagatcgcc caccagcgtt ttcatcagcg ttgatttacc gacgccgttg
    16501 gtacccagta ccgcaagttt ttcaccgacc tccagcagca ggttgagatt tttaaacagt
    16561 ggaccgttat caaacccttt ggtcagacct tccacttcca gcgcgttacg gaacagtttc
    16621 ttatcctgtt caaaacggat gaacgggttc tgacggctgg aggctttcac ctcttccagt
    16681 ttgattttat caatctggcg cgcgcgcgaa gttgcctggc gagatttcga ggcgttggcg
    16741 ctaaagcggc taacgaaaga ttgcaactca gcaatctgcg ctttcttctt ggcgttatcg
    16801 gccagcagac gttcacgcgc ctgggtcgcc gccgtcatgt actcatcgta gttacccgga
    16861 taaacgcgca gctcgccgta atccagatcc gccatgtggg tacagaccat gttaaggaag
    16921 tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct cgttcagcac ctgttccagc
    16981 cagcgaatgg tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag aatatccgga
    17041 tcagcaaaca gcgcctgcgc cagaagcaca cgcagcttcc agccaggagc aacttcactc
    17101 atcgggccgt agtgctgttc cactggaatt cccacgccaa gcaacaattc accggcgcga
    17161 gcttccgcag agtaaccgtc catttcgccg tatttaactt ccagatcggc cactttatag
    17221 ccgtcttcct cactcatttc cggcaaagca tagatgcggt cacgctcctg cttcacttcc
    17281 cacaactctt tatgccccat gatcaccgta tccagcacag tgaactcttc aaaggcaaac
    17341 tgatcctgac gcagcttacc aatgcgctcg ttgggatcga gggaaacgtt acccaaagtc
    17401 ggctctaagt cgccgccgag gatcttcata aaggtggatt taccactacc gttcgcgcca
    17461 atcaggccgt aacggttgcc gccgccaaat ttgacggaaa tgttttcaaa caacggctta
    17521 ctgccgaact gcatggtgac gttactggaa actaacacag gcgtatcctg aaaagagata
    17581 tgacaaaccg cgcattatgc cataagtctg aaagaagatc ctgctcctcg cttaaggtct
    17641 acactcaagc caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac tgtgagctgg
    17701 aatgaactta taatgcgctt ccaatactct aatattctca acccaatggc ctgccaggca
    17761 caaaatctcg cttaacatga atatgaaatt gaaaacatta ttcgcagcgg ccttcgctgt
    17821 tgtcggcttt tgcagtaccg cctctgcggt aacttatcct ctgccaaccg acgggagtcg
    17881 cctggttggt cagaatcagg tgatcaccat tcctgaaggt aacactcagc cgctggagta
    17941 ttttgccgcg gagtaccaga tggggctttc caatatgatg gaagcgaacc cgggtgtgga
    18001 taccttcctg ccgaaaggcg gtactgtact gaacattccg cagcagctga tcctgccgga
    18061 taccgttcat gaaggcatcg tcattaacag tgcagagatg cgtctgtatt actatccgaa
    18121 agggaccaac accgttatcg tgctgccgat cggcattggt cagttaggca aagatacgcc
    18181 tatcaactgg accaccaaag ttgagcgtaa gaaagcaggc ccgacctgga cgccgaccgc
    18241 aaaaatgcac gcagagtacc gcgctgcggg cgaaccgctt ccggctgtcg ttccggcagg
    18301 tccggataac ccgatgggtc tgtatgcact ctacatcggt cgcctgtatg ctatccatgg
    18361 caccaacgcc aacttcggta tcggcctgcg tgtaagtcat ggttgtgtgc gtctgcgtaa
    18421 cgaagacatc aaattcctgt tcgagaaagt accggtcggt acccgcgtac agtttattga
    18481 tgagccggta aaagcgacca ccgagccaga cggcagccgt tatattgaag tccataaccc
    18541 gctgtctacc accgaagccc agtttgaagg tcaggaaatt gtgccaatta ccctgacgaa
    18601 gagcgtgcag acagtgaccg gtcagccaga tgttgaccag gttgttcttg atgaagcgat
    18661 taaaaaccgc tccgggatgc cggttcgtct gaattaatct tcaaaactta aagcaaaagg
    18721 cggactcata atccgccttt tttatttgcc agaccctagt tggccgggag tataactaac
    18781 aaaacatatc ccaccaacgc cgcccacaac agcatcggta ttgaatagag atggaaacgc
    18841 caccagatgc ggcgatcgtt cgccatacgt agcgcaatca aacttgccag cgatccgggt
    18901 aataacccaa agccacctac gtttaccgcc catgccagta ataaagacgg cggcacatag
    18961 ttcagcaaca atatggtact cggcacgtta ctgatcacct gcgataaacc gattgccgtt
    19021 aaccataacc cgggttcaga tagatgactc acgttaccca acacgccttg caacgctgga
    19081 agctgggtca gtaaatggac gtcgataaac atcgccataa acaccagcag cagcgtccag
    19141 tccacactga gcaccacgcg acgtgccagc agcgcaaagc ctgccgccac aatcaccagt
    19201 ccccacagct cttgtttgaa ctccagcgcc gttagaaaga cgatatacag ccccagacaa
    19261 ctccacacca gccgcggttt ccactccggt gtttgcaccc ccgtatggta ttgcagcacc
    19321 tttccaggga aacaacacca gcacaggagc aggagcgtca gcatcattgc gccagccagc
    19381 ggtgccattt gggcaataaa tccggcaaac gaaagaccag aacgtcccca gataagaata
    19441 ttttgcgggt tgccaattgg cgtcagtagc gaaccagcat tgactgccag cgcctcaaaa
    19501 ataatcagac gattaaccgg gatctcacac agtcttttta gcgtgatagt cagcggaaca
    19561 acaataaaca gcgcgacatc gttggtcaga aaggtagaaa gcagcgccgc cgccagcacc
    19621 ataaacatcg ccagccgacg ctccgtagca aagcggcgca ccattttgcg ccccagcaca
    19681 tcaaaataac cgcttaactc cacacctttg gtcagcagca tcaggccgct taaggtgatg
    19741 atggtgtgcc agtcgatagc agcaggccag gatttcggtg caaagggcac gaaaaagctt
    19801 aatccgatac caacaagaat taataactga aaaaaacgat cgccttgcag cgtgcgtaaa
    19861 aaaggcaggc tcatttggca ccgtgtttct gggtaaacaa acgaaaagcg tcgagcgtct
    19921 cttcactaac atggtgctcc atgccttccg cgtcgcgacg ggcgatttcc ggactgacgc
    19981 ccaacaccaa caagaaattt tcgactatct gatgtcgctc gcggctttcc tgcgccagct
    20041 tctctccttc tgccgttaaa aacacgccac gccaggggat catttcaatc agccccatgg
    20101 ttgccagccg cttaagcatt ttagccaccg tcggttgcga aactcccaga cgagcagcca
    20161 tgtccacctg acgagcttcc cccacttccc tgatcaagtc agaaatcagc tcaacgtaat
    20221 catcaataag ctcgcgccga tgcgcctctc tgacctggcg gaacccttca acgtgctctt
    20281 ccacgttcac taactgcgtc actttttttg ctgttggcgt acctgcgcga cgactcattg
    20341 tgcttcctca ttatggtgac gcatacatag cgttctcatt tcgtgcgcac attgtaaacc
    20401 agagttgcga aggtacaaaa aattaacgtt ttagcaatag ctatataata tagcctgtgc
    20461 tatatctgta tgtaatgcaa tcatccctca aggatcgacg ggattagcaa gtcaggaggt
    20521 cttatgaatg agttcaagag gtgtatgcgc gtgtttagtc attctccctt taaagtacgg
    20581 ttaatactgc tctctatgtt gtgcgatatg gtcaacaaca aaccgcagca agataaacct
    20641 tccgataaat agcggcgtcg cggtacgccg cttcactcct gctttcatgc aggcataacg
    20701 cgttttggcc tgaaaaaccc cactttttgt cggatttgca atccccttcg caaaagattt
    20761 gttcgtcagt agttgacctg aacggcggct cgctctatct tcttgcagcc ctgcgtatat
    20821 tgcggctcgc ggatgcggac ccctttccac tcttcacgca ctcttgcagg tattgaccct
    20881 tgacgccagg gtaagcacat ggcgtttgtt acgatagtgg catatgaatt taaccctcaa
    20941 agaatcgctt gttacccgta gccgggtatt tagcccgtgg actgcgttct actttttaca
    21001 gtcgctatta attaacctcg gcttaggtta ccccttcagt ttgctctaca ccgctgcgtt
    21061 tacggctatt ttgcttttgc tatggcgaac attgcctcgc gtacaaaaag ttctggtcgg
    21121 tgtcagttcg ctggtggcgg cttgttattt cccttttgct caggcctacg gcgcgcctaa
    21181 tttcaataca ttgctggcat tgcactccac caatatggaa gagtcgaccg aaatcctgac
    21241 gatttttccg tggtacagct acctggtcgg cttatttatt tttgcgctcg gcgtaatagc
    21301 aatcaggcga aaaaaagaga atgaaaaagc gcgctggaat accttcgaca gcctgtgtct
    21361 ggtattcagt gtggcgacat tttttgttgc tcccgtgcaa aacctggcct ggggtggcgt
    21421 atttaaactg aaagatactg gctatccggt atttcgtttt gctaaggatg tcatcgtcaa
    21481 taataacgag gtgattgaag agcaagaacg gatggcaaaa ctttccggaa tgaaagatac
    21541 ctggacggtc actgccgtta agccgaagta tcagacctat gtggtggtga tcggtgaaag
    21601 cgcgcgtcgc gatgccctcg gtgcctttgg cggtcactgg gacaataccc cgtttgccag
    21661 cagcgttaac ggtttgatat ttgctgacta cattgccgcc agtggctcca cgcagaaatc
    21721 gcttggctta acgctcaatc gcgtagtcga tggcaaacca cagtttcagg ataactttgt
    21781 caccctggca aatcgcgcgg gcttccagac ctggtggttt tccaaccagg gccaaatcgg
    21841 cgaatacgat actgctatcg ccagcatcgc caaacgtgca gatgaagtgt acttcctgaa
    21901 agaaggtaat tttgaagcag ataaaaacac gaaagacgaa gcgttactgg atatgaccgc
    21961 tcaagtgctg gcgcaagagc actcgcaacc gcagctgatt gttctgcatc tgatgggctc
    22021 gcatccgcag gcctgcgaca ggacacaagg caaatacgaa acctttgtgc aatcgaaaga
    22081 aacgtcgtgc tatctctata ccatgacgca aacggacgat ttactgcgca agctgtacga
    22141 tcagttacgc aacagcggca gcagcttctc tctggtttac ttttctgacc acggtctggc
    22201 ctttaaagag cgcggcaaag acgtgcaata ccttgcccat gatgataaat atcagcaaaa
    22261 tttccaggtg ccttttatgg tcatttccag cgacgataaa gcgcatcggg tgattaaagc
    22321 ccgccgctca gccaatgact tcttaggctt tttctcgcag tggacgggaa ttaaagcgaa
    22381 ggaaataaat atcaaatacc cgtttatatc tgagaagaaa gccgggccga tatacatcac
    22441 caacttccag ttacagaagg tggattacaa ccatctcgga accgatattt tcgacccgaa
    22501 accttaaaaa caaaaatccg ccccgagagg cggatttttt atatcaccaa agtgattaga
    22561 agcggtaacc aacaccggca atccaggtgc ctacgtcaac gctacgaata cggctctgct
    22621 cgtaagagaa gtccagagca acgttttcca tcgggttgaa ctgcagacca gcaccgtagg
    22681 agaaaccgta gtcgctggtg tcgtgtttgt aggtcgggta ttcagtggtc tggaatttac
    22741 cataacccac acccactaca ccgtagatgc ttgcccagtc gttaatgcgg taagccggac
    22801 cagcagtgat gccatagtac tggtttttgt tgtagtcacc agagcttgca gtacgacttt
    22861 tctcggtgta agtgaaagaa ccgatcacac ccagcgggct gttgtcttct tcatagcggt
    22921 atttcaggtt gaaaccgccc attttgttca tttggccctg agcgtcgctc tgtgcgtaac
    22981 cgccagttac ggtagaagtc gcagctacgg aagtacctgc ggtgaaagcc agaactgcgg
    23041 ccagtgctga aagacatgca atttttttca taaccacctc aaatgtgatt caaataagtc
    23101 ctaagtttta aatatatcaa aaattaatgg gaaactcttc gcgatttgtg atgtctaacg
    23161 ggccatttca tgtaacagaa cgtttccata caccgctatc catctaaatt taaatcactt
    23221 tttcagagaa ctgcgtaagt attacgcatg ttttccctgt cattcatcca gattattcct
    23281 aatcaccaga ctaatgattc catcaatcct ggcgcatttt agtcaaaacg ggggaaaatt
    23341 ttttcaacaa atgctcaacc agcattgggt atatccagta cactccacgc tttacttaag
    23401 tctagatatt tgtgggagaa aggatgcctg gttcattacg taaaatgccg gtctggttac
    23461 caatagtcat attgctcgtt gccatggcgt ctattcaggg tggagcctcg ttagctaagt
    23521 cactttttcc tctggtgggc gcaccgggtg tcactgcgct gcgtctggca ttaggaacgc
    23581 tgatcctcat cgcgttcttt aagccatggc gactgcgctt tgccaaagag caacggttac
    23641 cgctgttgtt ttacggcgtt tcgctgggtg ggatgaatta tcttttttat ctttctattc
    23701 agacagtacc gctgggtatt gcggtggcgc tggagttcac cggaccactg gcggtggcgc
    23761 tgttctcttc tcgtcgcccg gtagatttcg tctgggttgt gctggcggtt cttggtctgt
    23821 ggttcctgct accgctgggg caagacgttt cccatgtcga tttaaccggc tgtgcgctgg
    23881 cactgggggc cggggcttgt tgggctattt acattttaag tgggcaacgc gcaggagcgg
    23941 aacatggccc tgcgacggtg gcaattggtt cgttgattgc agcgttaatt ttcgtgccaa
    24001 ttggagcgct tcaggctggt gaagcactct ggcactggtc ggttattcca ttgggtctgg
    24061 ctgtcgctat tctctcgacc gctctgcctt attcgctgga aatgattgcc ctcacccgtt
    24121 tgccaacacg gacatttggt acgctgatga gcatggaacc ggcgctggct gccgtttccg
    24181 ggatgatttt cctcggagaa acactgacac ccatacagct gctggcgctc ggcgctatca
    24241 tcgccgcttc aatggggtct acgctgacag tacgcaaaga gagcaaaata aaagaattag
    24301 acattaatta aatttacatt tctgcatggt tatgcataac catgcagaat ttctcgctac
    24361 ttttcctcta caccgtcttt atatatcgaa ttatgcaaaa gcatatttat tccgaaaatt
    24421 cctggcgagc agataaataa gaattgttct tatcaatata tctaactcat tgaatcttta
    24481 ttagttttgt ttttcacgct tgttaccact attagtgtga taggaacagc cagaatagcg
    24541 gaacacatag ccggtgctat acttagtctc gttaattact gggacataac atcaagagga
    24601 tatgaaatta tgagtaccgc taaattagtt aaatcaaaag cgaccaatct gctttatacc
    24661 cgcaacgatg tctccgacag cgagaaaaaa gcaacagtag agttgctgaa tcgccaggtt
    24721 atccagttta ttgatctttc tttgattacc aaacaagcgc actggaacat gcgcggcgct
    24781 aacttcattg ccgtacatga aatgctggat ggcttccgca ccgcactgat cgatcatctg
    24841 gataccatgg cagaacgtgc agtgcagctg ggcggtgtag ctctggggac cactcaagtt
    24901 atcaacagca aaaccccgct gaaaagttac ccgctggaca tccacaacgt tcaggatcac
    24961 ctgaaagaac tggctgaccg ttacgcaatc gtcgctaatg acgtacgcaa agcgattggc
    25021 gaagcgaaag atgacgacac cgcagatatc ctgaccgccg cgtctcgcga cctggataaa
    25081 ttcctgtggt ttatcgagtc taacatcgaa taaatccatc gctgatggtg cagaacttta
    25141 gtacccgata aaagcggctt cctgacagga ggccgttttg ttttgcagcc cacctcaacg
    25201 cacttattta gtgcatccat ctgctatctc cagctgatta agtaaatttt ttgtatccac
    25261 atcatcacac aatcgttaca taaagattgt tttttcatca ggttttacgc taaataatca
    25321 ctgtgttgag tgcacaattt tagcgcacca gattggtgcc ccagaatggt gcatcttcag
    25381 ggtattgccc tataaatcgt gcatcacgtt tttgccgcat ctctaaaaat caatgagttg
    25441 caaaactggc acgatttttt catatatgtg aatgtcacgc aggggatcgt cccgtggata
    25501 gaaaaaagga aatgctatga agtctgtatt aaaagtttca ctggctgcac tgaccctggt
    25561 ttttgcggtt tcttctcatg ccgcggataa aaaattagtt gtcgcgacgg ataccgcctt
    25621 cgttccgttt gaatttaaac agggcgataa atatgtgggc tttgacgttg atctgtgggc
    25681 tgccatcgct aaagagctga agctggatta cgaactgaag ccgatggatt tcagtgggat
    25741 cattccggca ctgcaaacca aaaacgtcga tctggcgctg gcgggcatta ccatcaccga
    25801 cgagcgtaaa aaagcgatcg atttctctga cggctactac aaaagcggcc tgttagtgat
    25861 ggtgaaagct aacaataacg atgtgaaaag cgtgaaagat ctcgacggga aagtggttgc
    25921 tgtgaagagc ggtactggct ccgttgatta cgcgaaagca aacatcaaaa ctaaagatct
    25981 gcgtcagttc ccgaacatcg ataacgccta tatggaactg ggcaccaacc gcgcagacgc
    26041 cgttctgcac gatacgccaa acattctgta cttcatcaaa accgccggta acggtcagtt
    26101 caaagcggta ggtgactctc tggaagcgca gcaatacggt attgcgttcc cgaaaggtag
    26161 cgacgagctg cgtgacaaag tcaacggcgc gttgaaaacc ctgcgcgaga acggaactta
    26221 caacgaaatc tacaaaaaat ggttcggtac tgaaccgaaa taataacgct acacctgtaa
    26281 agcgcactgg cagttccctc tcccctctgg ggagaggatt agggtgaggg gcgcaaaccc
    26341 gctccggggc cattaattac cctgaatttg attatttaca ccacggtaac aggaacaaca
    26401 tatgcagttt gactggagtg ccatctggcc tgccattccg cttctgattg aaggtgccaa
    26461 aatgaccctg tggatttcgg tcctcggtct ggctggcggt ctggtaatcg gattgctggc
    26521 aggttttgca cgcaccttcg gtggttggat agccaaccac gtcgcgctgg tctttattga
    26581 agtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt atctatttcg ccctgccgat
    26641 ggcgtttaac gacttacgca tcgacccatt tactgcggcg gtggtcacca tcatgatcaa
    26701 ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg ctgtctattc acaaaggttt
    26761 tcgtgaagca ggactggcgc tcggtctttc acgttgggaa accattcgct acgtcatttt
    26821 accgctggca ctgcgtcgta tgctgccgcc gctgggtaac cagtggatca tcagcattaa
    26881 agacacctcg ctgtttattg ttatcggcgt ggcggaactg acccgtcagg ggcaagaaat
    26941 tattgccggt aacttccgcg cccttgagat ctggagcgcc gtggcggtgt tctatctgat
    27001 tattaccctg gtgctgagct ttattctgcg tcgtctggaa agaaggatga aaatcctgtg
    27061 attgaattta aaaacgtctc caagcacttt ggcccaaccc aggtgctgca caatatcgat
    27121 ttgaacattg cccagggcga agtcgtggtg attatcgggc cgtccggttc cggtaaatcg
    27181 accctgctgc gctgcatcaa caaactggaa gaaatcacct ccggcgatct gattgtcgat
    27241 ggcctgaagg ttaacgatcc gaaagttgac gagcgcctga ttcgccagga agcaggtatg
    27301 gtgttccagc agttttacct cttcccgcat ctgacggcgc tggaaaacgt catgtttggt
    27361 ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa aactggcgcg tgagctgctg
    27421 gcgaaagtcg gtctggcaga acgtgcacat cactaccctt ccgaactttc tggtggtcaa
    27481 cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc cgaaaatgat gctgtttgat
    27541 gaaccgactt ccgctcttga cccggaactg cgccatgaag tgctgaaggt tatgcaggat
    27601 ctggctgaag aagggatgac gatggtgatc gtgacccacg aaatcggttt tgccgagaaa
    27661 gtagcttcgc ggctgatctt tatcgacaaa ggccggattg cggaagatgg caatccgcag
    27721 gtgttgatca agaacccgcc gagccagcgc ttgcaggaat ttttgcagca cgtct
//
LOCUS       DAJTNN010000036        27518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTNN010000036 DAJTNN010000000
VERSION     DAJTNN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.036"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(174..704)
                     /gene="ppa"
                     /locus_tag="OO150_001381"
     CDS             complement(174..704)
                     /gene="ppa"
                     /locus_tag="OO150_001381"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5515578.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            1014..1970
                     /gene="ytfQ"
                     /locus_tag="OO150_001382"
     CDS             1014..1970
                     /gene="ytfQ"
                     /locus_tag="OO150_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR5515579.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENRIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            2110..3612
                     /locus_tag="OO150_001383"
                     /pseudo
     CDS             2110..3612
                     /locus_tag="OO150_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            3626..4648
                     /locus_tag="OO150_001384"
     CDS             3626..4648
                     /locus_tag="OO150_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5515580.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            4635..5630
                     /gene="yjfF"
                     /locus_tag="OO150_001385"
     CDS             4635..5630
                     /gene="yjfF"
                     /locus_tag="OO150_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR5515581.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="OO150_001386"
     CDS             complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="OO150_001386"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5515582.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            6837..8210
                     /gene="mpl"
                     /locus_tag="OO150_001387"
     CDS             6837..8210
                     /gene="mpl"
                     /locus_tag="OO150_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5515583.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(8293..8844)
                     /locus_tag="OO150_001388"
     CDS             complement(8293..8844)
                     /locus_tag="OO150_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5515584.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            8938..10290
                     /gene="pmbA"
                     /locus_tag="OO150_001389"
     CDS             8938..10290
                     /gene="pmbA"
                     /locus_tag="OO150_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5515585.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGVDCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            10474..10860
                     /gene="cybC"
                     /locus_tag="OO150_001390"
     CDS             10474..10860
                     /gene="cybC"
                     /locus_tag="OO150_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR5515586.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="OO150_001391"
     CDS             complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="OO150_001391"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5515587.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(11468..13606)
                     /gene="nrdD"
                     /locus_tag="OO150_001392"
     CDS             complement(11468..13606)
                     /gene="nrdD"
                     /locus_tag="OO150_001392"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5515588.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYSNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(14000..15655)
                     /gene="treC"
                     /locus_tag="OO150_001393"
     CDS             complement(14000..15655)
                     /gene="treC"
                     /locus_tag="OO150_001393"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5515589.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(15705..17126)
                     /gene="treB"
                     /locus_tag="OO150_001394"
     CDS             complement(15705..17126)
                     /gene="treB"
                     /locus_tag="OO150_001394"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5515590.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNARQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(17227..18192)
                     /gene="treR"
                     /locus_tag="OO150_001395"
     CDS             complement(17227..18192)
                     /gene="treR"
                     /locus_tag="OO150_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5515591.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            18377..18430
                     /gene="mgtL"
                     /locus_tag="OO150_001396"
     CDS             18377..18430
                     /gene="mgtL"
                     /locus_tag="OO150_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5515592.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            18571..21267
                     /gene="mgtA"
                     /locus_tag="OO150_001397"
     CDS             18571..21267
                     /gene="mgtA"
                     /locus_tag="OO150_001397"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5515593.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(21473..21859)
                     /gene="ridA"
                     /locus_tag="OO150_001398"
     CDS             complement(21473..21859)
                     /gene="ridA"
                     /locus_tag="OO150_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5515594.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYETFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(21932..22393)
                     /gene="pyrI"
                     /locus_tag="OO150_001399"
     CDS             complement(21932..22393)
                     /gene="pyrI"
                     /locus_tag="OO150_001399"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5515595.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(22406..23341)
                     /gene="pyrB"
                     /locus_tag="OO150_001400"
     CDS             complement(22406..23341)
                     /gene="pyrB"
                     /locus_tag="OO150_001400"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5515596.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(23345..23479)
                     /gene="pyrL"
                     /locus_tag="OO150_001401"
     CDS             complement(23345..23479)
                     /gene="pyrL"
                     /locus_tag="OO150_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5515597.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(23759..24154)
                     /locus_tag="OO150_001402"
     CDS             complement(23759..24154)
                     /locus_tag="OO150_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5515598.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     SYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(24285..24998)
                     /gene="bdcA"
                     /locus_tag="OO150_001403"
     CDS             complement(24285..24998)
                     /gene="bdcA"
                     /locus_tag="OO150_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5515599.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            25069..25662
                     /locus_tag="OO150_001404"
     CDS             25069..25662
                     /locus_tag="OO150_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5515600.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            25807..26259
                     /gene="tabA"
                     /locus_tag="OO150_001405"
     CDS             25807..26259
                     /gene="tabA"
                     /locus_tag="OO150_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5515601.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            26382..27518
                     /locus_tag="OO150_001406"
                     /pseudo
     CDS             26382..27518
                     /locus_tag="OO150_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         6598 a         6813 c         7279 g         6828 t
ORIGIN      
        1 tccagcaagt ggctatgaac agcaaaatgg tggtcgggtt tgcttgttct taccgttcaa
       61 gccttgcaac cgctgagaat aatgatggtg gaatagagag tttatttatc gcgggcataa
      121 aaaaaccctt actaaccgaa gcccggcgtt cagggttatt gcgctagaag aacttattta
      181 ttctttgcgc gctcgaagga agcaacgatt tcagctttag cggcttctgc gttttcccaa
      241 ccttcaactt tcacccactt gcctttttcg aggtctttgt agtgctcgaa gaagtgagcg
      301 atttgcgctt tcagcagttc aggcagatcg tttacgtctt taatgtgatc gtattctttg
      361 ctcagcttgc tgtgcggaac cgcaaccagt ttcgcatctt caccggcttc gtcggtcatt
      421 ttcagaacgc caaccggacg gcaacggatc acagaaccag gctgcagcgg gtacggagtc
      481 gggaccagta cgtcaaccgg gtcaccgtcc agagacaggg tgtggttgat gtaaccgtag
      541 ttgcacggat agaacatcgc ggtggacatg aagcggtcaa cgaacagtgc gccgctctct
      601 ttgtcgattt cgtatttgat cggatctgcg ttagccggga tctcaataac aacgtagatg
      661 tcttccggca gatctttacc cgcagggacg ttgagtaagc tcatgtctgt ttcctttaaa
      721 aatatgtggc aaacaagtgc cgagtattat agccaactcg cgccgaatgt ctttgcttgt
      781 tttcgtcttc attttccttt ttcgccagtt ttaagacggt atacagagca ggaaaatcca
      841 taaccgtagc cgcatttttc atagtgagat gaaagcgatt acaaacttgt gattaacgtt
      901 ttatttactt ttttgaagtg tgatgtaacg caatccgtta cataacgaat tgtctatagt
      961 tttttcgcga acatcttttc accaataata attaccccga cgaggacaac cctatgtgga
     1021 aacgcttact tgtagtctct gcagtctcgg cagccatgtc gtctatggcg ttggccgctc
     1081 cattaaccgt aggattttcg caggtcggat cggaatccgg ctggcgcgcc gcagaaacca
     1141 atgtggcgaa aagtgaggcc gaaaaacgcg gaattacgtt gaaaattgcc gatggtcagc
     1201 aaaagcagga aaaccggatt aaagcggtac gttccttcgt tgcacaaggg gtggatgcga
     1261 tctttatcgc tccagtggtt gcgacgggtt gggaaccggt attaaaagag gcgaaagatg
     1321 ccgaaatccc ggtcttcttg ctcgaccgtt ccatcgatgt gaaagacaaa tctctctata
     1381 tgaccaccgt tactgccgac aacatcctcg aaggtaagtt gatcggtgac tggctggtaa
     1441 aagaagtgaa tggcaaacca tgcaacgtgg tggagctgca gggcaccgtt ggggccagcg
     1501 tcgccattga ccgtaagaaa ggctttgccg aagccattaa gaatgcgcca aatatcaaaa
     1561 ttatccgctc gcagtcaggt gacttcaccc gcagtaaagg caaagaagtc atggagagct
     1621 ttatcaaagc ggaaaacaac ggcaaaaaca tctgcatggt ttacgcccat aacgacgaca
     1681 tggtgattgg tgcaattcag gcaattaaag aagcgggcct gaaaccgggc aaagatattc
     1741 tgacaggttc tatcgatggc gtaccggata tctacaaagc gatgattgat ggcgaagcga
     1801 acgccagtgt tgaactgacg ccgaatatgg cgggacccgc ttttgacgcg ctggagaaat
     1861 acaaaaaaga cggcaccatg cctgaaaagc tgacgttaac caaatccacc ctttacctgc
     1921 ctgataccgc aaaagaagag ttagagaaga agaaaaatat ggggtattga gggttgctat
     1981 gcctgatgcc gattcgtagg ccggataagg cgctcgcgcc gcatccggcg atggtgcact
     2041 gaagcctgat gcgacgctta ccgcgtctta tcatgcctac tgggagcacg ctttacaccg
     2101 ggggaaacca tgacgaccga ccaacaccag gaaatcctcc gcaccgaagg attaagtaaa
     2161 tttttccccg gcgtcaaagc gttagacaac gttgatttca gcctgcgccg tggcgaaatc
     2221 atggcgctgc tcggtgaaaa cggggcggga aaatcaacgc taatcaaagc attaactggt
     2281 gtataccacg ccgatcgcgg caccatctgg ctggaaggcc aggttatctc accgaaaaat
     2341 accgcccacg cgcaacaact cggcattggc accgtctatc aggaagtcaa cctgctaccc
     2401 aatatgtcgg tcgctgataa tctatttata ggccgcgaac ccaaacgctt cggccttcta
     2461 cgccgcaaag agatggaaaa gcgcgccacc gaactgatgg catcttacgg tttctccctc
     2521 gacgtgcgcg aaccgctcaa ccgcttttca gtcgcgatgc agcaaatcgt cgccatttgc
     2581 cgggctatcg atctctccgc caaagtgctg atcctcgatg aacccaccgc cagcctcgac
     2641 acctaggaag tggagctatt atttggcctg atgcgtcagt tgcgcgatcg cggcgtcagc
     2701 ctgatctttg tcactcactt tctcgatcag agctatcagg tcagcgatcg gatcaccgtt
     2761 ttacgcaacg gcagtttcgt aggctgtcgg gaaacgcgcg agctaccgca gatcgaactg
     2821 gtaaaaatga tgctggggcg cgagctggat acccacgcgc tacagcgtgc cgggcgaaca
     2881 ttgttgagcg acaaacccgt tgccgcgttc aaaaattacg gcaaaaaagg aacgatagca
     2941 ccgtttgatc tcgaagtgcg ccccggcgag atcgtcggcc tggcaggctt gctgggatcc
     3001 ggacgtaccg aaaccgccga agtgatcttc ggtatcaaac ctgctgacag cggcacggcg
     3061 ttgatcaaag gcaaaccgca aaacctgcga tcgccacatc aggcttcggt actgggcatc
     3121 ggcttctgcc cggaagacag gaaaaccgat ggcatcatcg ctgccgcctc ggtgcgggaa
     3181 aatatcatcc tcgctctcca ggcccagcgc ggctggctac gacccatttc ccgcaaagag
     3241 cagcaagaga ttgccgaacg ctttatccgc cagcttggca ttcgcacacc ttcaactgaa
     3301 caaccgattg aatttctctc cggcggcaat cagcaaaaag tgttgctttc acgttggcta
     3361 ctgacccgac cgcaatttct gatcctcgat gagccaaccc gtggcattga tgttggtgcc
     3421 cacgccgaga tcatccgcct gattgaaacg ctatgcgccg atggtctggc gctgctggtg
     3481 atctcctccg aactggaaga actggtgggc tatgccgacc gggtgatcat catgcgcgat
     3541 cgcaaacagg tggcggagat ccggctggca gagctttccg ttccggctat catgaacgcc
     3601 attgcggcgt aaggagaaca gtgtgatgcc tcaatctctt ccggacacca ctacgccaaa
     3661 aaggcgcttt cgctggccga cggggatgcc gcagctggta gcactattgc tggtgctgct
     3721 ggtcgacagc ctggtagccc cgcatttctg gcaggtagtg cttcaggatg ggcgtttgtt
     3781 cggtagcccc atagacattc ttaaccgtgc agcccccgtt gcgttactgg cgattggtat
     3841 gacgctggtg atcgccaccg gtgggattga tctctccgta ggggcggtga tggctatcgc
     3901 cggagccaca acggctgcga tgacggtcgc ggaagtcagc ctgccgattg ttttgttaag
     3961 cgccctaggc accggcatcc tggcgggatt gtggaacggc atactggtag cgatcctcaa
     4021 aattcagcca tttgtcgcca ccctgatcct gatggtcgcc gggcgcggcg tggcgcaact
     4081 gatcacctcc ggacagatcg tcacgtttaa ctcgccggat ctctcatggt ttggcagtgg
     4141 atcgctgttg ttcctgccaa cgccagtcat tattgcggta ctgacgcttc tcctgttctg
     4201 gctgttgacc cgcaaaacgg cactggggat gtttatcgaa gccgttggta tcaacattcg
     4261 ggcggcaaaa aatgccgggg taaacacgcg gatcatcgtc atgctcactt atgtgttgag
     4321 cgggctgtgt gcggcgattg cgggcattat cgtggcggcg gatattcgcg gtgccgatgc
     4381 caacaacgcc ggattatggc tggagctgga cgccattctc gcggtggtga ttggcggcgg
     4441 atcgctgatg ggcgggcgct ttaacctgct gctctcggta gtgggggcgc tgattattca
     4501 ggggatgaac accggaattt tgctttcggg ctttccaccg gagatgaacc aggtggtgaa
     4561 agcggtggtg gtgctttgcg tgctgattgt ccagtcgcaa cgctttatca gtctgattaa
     4621 aggagtacgt agccgtgata aaacgtaatt tgccactgat gatcaccatc ggcgtctttg
     4681 tgctgggcta tttctactgc ctgacgcagt ttcccggttt tgcttccaca agagtgatct
     4741 gcaatatcct gaccgataac gcttttctgg ggattattgc cgttggtatg acctttgtga
     4801 tcctctctgg cggtatcgat ctctccgtcg gttcggtgat cgcctttact ggcgtgtttc
     4861 tggcaaaagt ggttggcgat ttcggcctct cgccgctgct ggcgtttccg ctagtgctgg
     4921 tgatgggctg tgccttcggc gcatttatgg ggcttctgat cgacgccctg aagatcccgg
     4981 catttatcat tacgcttgcg gggatgttct ttttgcgcgg cgtcagctat ctcgtttcgg
     5041 aagagtcgat tccgataaac catcccattt atgacacgct ctcaagcctt gcgtggaaaa
     5101 tccccggcgg cggtcgcctt agtgcgatgg ggctgttgat gctggcggtg gtggttatcg
     5161 gtattttcct tgcgcatcgt acccgttttg gcaatcaggt gtacgctatt ggcggcaacg
     5221 cgacgtcggc gaacctgatg gggatttcta ctcgcagcac tactattcgc atttatatgc
     5281 tctccaccgg actggcaaca ctggcgggga ttgtcttctc gatttatacc caggccggat
     5341 atgcactggc gggcgtaggt gtggaactgg acgccatcgc gtcggtggtg attggcggta
     5401 cgttgttaag tggtggcgtc gggacggtgc ttgggacgct ttttggtgtg gcaattcagg
     5461 gactgatcca gacttacatc aactttgacg gcacgctgag ttcctggtgg acgaaaatcg
     5521 ccatcggtat tttgttgttt atttttatag cattacagcg tgggttaacg gtgctgtggg
     5581 agaatcgtca gagttcgcca gtgacaagag tcaacattgc gcagcgataa aaatgcctct
     5641 ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag cgttcgacat
     5701 cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcaga gtttccggga
     5761 tgatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt tccgccagga
     5821 atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg ctggcggtgc
     5881 ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca accagtgaac
     5941 cgatataacg tgaggtatac gggcggttgg tggatttatc ttcttcctgg cagaatttaa
     6001 tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg gagtaggttt
     6061 tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc agcgaaggat
     6121 cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta gaggagccgt
     6181 ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct tcttccgtta
     6241 ccggcgtgcc gacgggcgta actcggcggt agatggagaa aatggtaccg acagagacgt
     6301 taacatcgat gttggacgag ccatccagtg ggtccatcag caccacgtat tttgcatgtt
     6361 cacagccttc aaagacaaca atctcatctt cttcttcaga ggcaatgcct gcaacgatat
     6421 cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt ttttgctgaa
     6481 cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt cctgctttgt
     6541 tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa gcagtgagct
     6601 caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct aacgttttca
     6661 taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta acaaataatc
     6721 tcaatgttgc gctcaggtga atcgcgccag caaattacgg attatcctga aatgcgtttc
     6781 tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag tgacgtatgc
     6841 gcattcatat tttaggaatt tgtggcacgt ttatgggcgg tctggcgatg ctggcgcgcc
     6901 agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg agcaccttac
     6961 ttgagaagca aggcattgaa ctgattcagg gttacgatgc cagccagctc gatccgcagc
     7021 cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg gaagcggtac
     7081 tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt gtgctgcgcg
     7141 accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg ggaatggcga
     7201 cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt gtgccgggga
     7261 actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa gcggatgagt
     7321 acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg cgtacgctga
     7381 tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa gcgatccaga
     7441 aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc tggccggaaa
     7501 atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag gagctggtgg
     7561 gtgagcaggg gcactggcag gcgaaaaagc tgaccactga tgcttccgaa tgggaagtct
     7621 tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa cataatatgc
     7681 ataatggcct gatggcgatt gcggcggccc gccatgttgg cgtagcgccg gcagataccg
     7741 ctaacgcgct gggttcgttt attaatgctc gtcgccgtct ggagttgcgt ggtgaagcga
     7801 atggcgtcac ggtatatgac gattttgccc atcacccgac ggcgattctg gcaacgctgg
     7861 cggcgctgcg tggcaaagtt ggtggtacag cgcgcattat tgctgtgctg gaaccgcgct
     7921 cgaataccat gaaaatgggg atctgcaaag acgatttggc accttcatta ggtcgtgccg
     7981 atgaagtctt cctgctgcaa ccggcgcata ttccgtggca ggtggcagaa gtggcagaag
     8041 cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat atggtggtga
     8101 aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt ggtgggatcc
     8161 atcagaaact gctggatggt ctggcgaaga aggcggaagc tgcgcagtaa ttcggcctca
     8221 gcctgagata gcattgccgg ataaggcgtt tacgccgcat ccggcaaacc atactcacat
     8281 caacaacgaa aattactctt cattctccgc cagttcgcgc agatactgga aaatctggcg
     8341 tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttgcgga tcagagtacg
     8401 cagttgctga cgatcggcat cgggccacag atttaatacc tcagcgatgg cgtcatcacc
     8461 ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca cctgctggtt
     8521 gtgacggttt ttcagcttat ccagcgcctg acggatcggt tctacatcgc gctggcgcag
     8581 catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac gctgggcgag
     8641 ctcaatagcc gcgcgcagat ccgcatctaa cgggatctta tccagcgcgt ttttccccag
     8701 atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa tttcactttt
     8761 actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa cgtcgtcgag
     8821 ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag
     8881 ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg
     8941 gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca
     9001 gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc
     9061 aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat
     9121 agcgatggcg cgctggggat cactgtttat caccagaacc gcaaaggtag cgcatcatcc
     9181 accgatttaa gcccgcaggc cattgcccgt actgtacagg cggcgctgga tattgcccgt
     9241 tatacctcgc cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca
     9301 ccagatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg
     9361 gcccgcgcag aacaggcggc attgcaggcg gacaaacgca tcaccaatac cgaaggtggc
     9421 agctttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt
     9481 tactgctcaa cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg
     9541 gagcgtgatt acgcctacac cattggtcgt gcgatgagcg atctgcaaac gccagagtgg
     9601 gttggggtcg actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc
     9661 atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg
     9721 ggggcgatag cgggtggatc ggtttaccgt aaatctacct tcctgctgga ttcgctgggt
     9781 aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg
     9841 gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat
     9901 ggcatcctga ctcagtggct gctgaccagc tattcggcgc gaaaactggg gctgaaaagc
     9961 accggacatg cgggcggtat tcacaactgg cggattgccg gacaaggtct aagcttcgag
    10021 cagatgctca aagagatggg caccgggctg gtggtgacgg aattgatggg ccagggcgtg
    10081 agtgcaatta ccggtgatta ttcccgtggt gcggcgggct tctgggtaga aaacggcgaa
    10141 attcagtatc cggttagcga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat
    10201 attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg
    10261 ttgccggaga tgaaaatcgc cggacagtaa aaaaactggc gcgacctgtc gcgctataaa
    10321 ctaaaaaatt acacaaaatc attcgcactg catcgagacg gaaagtgaat gagggccggg
    10381 gagcgtactc gcagtacgtg acccggtcga atgagcgtag tcgtcgatga ggcagtgtga
    10441 atgatgaagt gtaaataaaa aaggaagtga gcaatgcgaa aaagcctgtt agctattctt
    10501 gcagtctcct cgttggtatt cagttcggcg tcgtttgccg ctgatcttga agacaatatg
    10561 gaaaccctca acgacaattt aaaagtggtc gaaaaagccg ataacgcggc gcaagtcaaa
    10621 gacgcgttaa cgaagatgcg cgccgcagcg ctggatgcgc aaaaagcaac gccgccgaag
    10681 ctcgaaggca aatcaccgga cagcccggag atgaaagact tccgtcacgg tttcgatatt
    10741 ctggtcggtc agattgacga cgcgctgaag ctggcaaatg aaggtaaagt aaaagaagcg
    10801 caggctgccg cagagcaact gaaaacgacc cgcaacgcct atcaccagaa gtatcgttaa
    10861 ttcctcattt ccctgttgcc tgcactcagg taacagggaa cttatcatcg caaatgatgc
    10921 accacctgat tgctgctgcc gcgccagatc agggaagggt cttttaaatc ctgcacaaat
    10981 ttgccgtcga ccagcacgtt aatcaaatca acaacctgca tttgcgctgc gttgagttcg
    11041 tcgagtttat agcctgtcca cacccagatg tctttacccg gacactcggc gcggatgcgt
    11101 tgtaccagtt tcagaatatc cggcacgttt tgcggatgca gcggatcgcc gccggagagg
    11161 gaaataccct ggcgtttgat acgagtgtca ttcagatcgt taatgatctg gtcttccatt
    11221 gctttagtaa atggctgacc ggaatttacc cgccatgtgc ttttgttata gcaaccgggg
    11281 cattcatgaa cacacccgga gacaaacaga gtgcaacgag tgccggggcc gttgacgatg
    11341 tcgacaggat agtactgatg ataattcatt atttcgcctg gcttatcagt ccgcgtgtag
    11401 actgcatccg gtaatcaatg cctgatgcga cgctttcaca tcacatccgg catttgctta
    11461 gtgcagatca acctatctgc ccatttccca aatgtttaac gcggcgctta acttcttcct
    11521 gcttgccggc gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag
    11581 ttaccgacac acgggaggcg tcatggttac cacatttcgg gcaggtgaag cctttgctgg
    11641 tgcactcgaa ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc
    11701 cgtaatacgg tacatgctga tagctgtaat cccagacatc ttccagcgcc ttcaggttgt
    11761 gctgaatgtt tgaatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag
    11821 gcgcttcaaa gtcgatcttg tcgtacgggt tcaccttctt ctccacatcg aggtggaaac
    11881 tgttggtgta gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga
    11941 gacggcagaa gcggtcgcac aggttttcac tcggcgtgct gtagagactg aaaccataac
    12001 ccgtttcttc cttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt
    12061 tcgcgcgaag ctgctcgttg tcgtaaacat gctcgccgcc gaacagcgcg ttaatggttt
    12121 cgtggatgcc gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa
    12181 catcatcgtc agcattgaga cgcacgccac aagcaccttc catatagagg atcggggcca
    12241 cgcgcgcttt cacgccttcg agacgagcga tacgggtcat cagcgcctta cgtgccagca
    12301 ccagacgttc atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa
    12361 tacgcggcag gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc
    12421 cgttttcatt ttcccacacg ccgaggaagc tgcggcagcc catcggagtt ttaaacgaac
    12481 cggtgacttt cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc
    12541 actccagcgc cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat
    12601 cgcgaatcgc aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg
    12661 cgatacggtt acgcaggata gattcctgaa tcaggcgcga ctcccagctg gtgcccagac
    12721 caaaaccaaa ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt
    12781 actccagcga ctggaaggca tcgtagcact ctttaatggt acgagagtta gcgtagcctt
    12841 cggcgtccgg gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtga
    12901 caaacggtgc cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg
    12961 cgacctgagc aataatctgc gcagttacag cggttgccgt agagatcgac ttcggcggtt
    13021 caatctcggc gttccccatc ttaaagccct gggtcagcat gcctttcagg tcgatcaaca
    13081 tgcagttgaa catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac
    13141 gctcatgtgc ctgcaccacg tcacgcggca gcaggtgctg acgtgcatag tgtttagcca
    13201 cgatcccggc cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt
    13261 cgttgagtaa cgaggcgttg gtctgctcga ccagaccacg gatctcctga ttcaggcgac
    13321 cgcgtttttc acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt
    13381 gtttgtatgg acctgacatc agctggtttt caacagcggt ctggatctca ttgatatcca
    13441 cctggttgcg gccctgcatc tgctcgctga caactgcagc aacagtggcg caataatcgg
    13501 catcatcgac ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt
    13561 taaacggcac tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt
    13621 tttaagaaca ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt
    13681 ttccggtgct ttccgcaatc gccatccgct tttccacaac gccgcacctt tttcgtgggg
    13741 agctgtagta gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag
    13801 tctatatata gtgctttgca tcaaggatgt ttgaactttt tttgatgtag ctcaaagtaa
    13861 aaagcagagc gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc
    13921 tggttgaaaa ataaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt
    13981 accggggctt agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat
    14041 tcatggcaca gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc
    14101 cgcgcatttg ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca
    14161 aggtttgccc cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat
    14221 cctggtaatt gccccatgtc aggatggctt cctgcttacg cagtgcgatt aacttttggt
    14281 aggtgtaaaa caccgaggaa tcatcggcca gcgcggcttc tacgttgatt tgttgatagt
    14341 tatcacccag gccaatccac ggttcgccag ccgtaaatcc ggcattatcg ccgttgctcc
    14401 attgcatggg cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt
    14461 cggcatcacg accatcgttg cgcagctcgg caaacatatt gaggctctct acgtcgcgat
    14521 agtcagtaat gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat
    14581 acggcgttcc ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt
    14641 attcaccttc atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca
    14701 aggcattcca tgctacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg
    14761 ccacaaagtc aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt
    14821 gatgaaaatt aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat
    14881 gctcaaggct ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat
    14941 cgcggttcat ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc
    15001 cgtcgccatc caggtcatca gggaaacgcg ggtctttgga gatcagattc accacatcca
    15061 ggcgcaaccc gtcgacccca cgatcggccc agaactcaca gacttttttc agcactgcgc
    15121 gtaccgctgg attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt
    15181 actgttcgct ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt
    15241 tcggtggcgt ttctggttct ccatcgcgcc agatataaaa ctggcggtaa gggctttctt
    15301 tgttcagcgc ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga
    15361 gaatgatacg aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg
    15421 tgccgtaggt gggatcaatc gccgtatagt tcgctacgtc gtaaccgtta tcgacctggg
    15481 gagagacata aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca
    15541 ggcgttggat aacgccacgt aaatcgccgg taccgctacc cgtggtgtcc tgaaaactct
    15601 ttggataaat ctggtagata acgccgtttt gccaccagtg gggaggatta gtcattacgt
    15661 tattcctgca aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc
    15721 gtgcccaggc ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg
    15781 gcgatagcca ttgccagcgc aaacacctgc cagtagttcg gttgaatcga gagaattccc
    15841 ggcaggccgc ctacgccgat gccattcgcc ataacgccgt tcaggccgca tagcaatcct
    15901 gccagaccag aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg
    15961 tacattgcag gctcagtgac cccaagccag gcggagatcg ccgcaggcac ggagatctcg
    16021 cgttcattgt gcttgcggct ggaaatgata atgccgatca cggcggagcc ctgagcgata
    16081 ttcgacagcg caatcagtgg ccacactggc gtaccaccca tgctttgaat catctgcaaa
    16141 tcaatagcaa gcgtggtctg gtgtaccccg gtgatcacca gcggggcgta caggaagcca
    16201 aacaatgccg cgccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca
    16261 acgccatcgc caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg
    16321 aggatcagcg aacagacggg caccaccacc agatagaggt aatctggcac gatgcgttta
    16381 aggcgagttt caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc
    16441 tgatagccca ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc
    16501 ccgagcagat aagcgttcat cagttggggg gaaaccagtg tcacgccaag cacgatacca
    16561 aggatcggtg tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag
    16621 aagaagatcg cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac
    16681 atttgcgcca gcgtttgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg
    16741 aggatcaaac cgccgctaat caacgcgggc agcaacggga agaaggtctc tgcgaaatga
    16801 gagatcaact gctcatgcca tttcatattc tgccgggcgg ctttttttac ctgctcttta
    16861 tcaacctgcg cctgtccggt tgacgctatc agtgcttgat agtagtcacc cacgttggtg
    16921 ccaatcacca cctgaaattg cctggcattg gtgaaacagc ctttcaccat ggggagttgc
    16981 tcaatttctt tcggtctggc attggccggt tggttgagga caaagcgtag gcgagtaata
    17041 cagtggctca ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata
    17101 tccgtttggt ttattttgct catcataaag ccccatggca gatgacattt ttggttggct
    17161 gcagaatata ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat
    17221 cacaatttat cgttcaggaa acgatctgga cagggtggcg gggatgatga tttgttgcgg
    17281 ttcgctgcgc ccggttacct gtgcgatcaa ctggcaggcc gcctggcgtc cagcttcggc
    17341 gtaaccggga tctacggtga cgatctccgg atggaggaat ttcattaacg gcgtattacc
    17401 gacgctcgcc agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag
    17461 tgccagcgtg tcggttgcgc acagtaaggc ggtagtttca ggcgtaatca cttttgctac
    17521 gttctcatag ccttgcttca tagcaaggcc tggcagagcg gcaacgggat gcagtttatg
    17581 cgctttgcag aacgccaggt aggcttcgtg acgtcgctta ccggtcgtca cgtcactgtg
    17641 cggcacgccg agataactga tattacgatg cccctggtca tacagccgtt gcatcaggat
    17701 cttgattgcc ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag
    17761 cagaaccagc gatgactgcc agtgggctaa catttcttct gttatgccgg taaaaccgaa
    17821 cagcactacg ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg
    17881 cggggaaaac tgactttcca tcatgattgg gtcgtaacct tgttcataga actctggcag
    17941 catggtttga acggcgagat tttctgacaa cgaatccagg cgggtaacaa tgatggcgac
    18001 cactttatcg ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg
    18061 attcatcact gcttcaacac gctcgcgggt gcgctggctc acgccgcttt cgttattcag
    18121 cacccgggaa actgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag
    18181 ccgattttgc atcctgttgt cctgtaacgt gttgtttaat tatttgagcc taactttacc
    18241 cgtgcattca gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta
    18301 tatcgccata gatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc
    18361 acttaccgga ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact
    18421 tttccggtaa gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaac
    18481 gtccctgctc agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt
    18541 actgtttcag acagtgcgga gggactcctt atgtttaaag aaatttttac ccggctcatt
    18601 cgccatttac cttcccgtct ggttcatcgt gatccattgc ctggcgcgca gcagacagtg
    18661 aatacggtgg tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa
    18721 gaagaattgt ggaaaacgtt cgacacccat ccggaagggt taaatcaggc ggaagtggaa
    18781 tctgcccgcg aacaacatgg tgaaaataaa ttacccgcac aacaactgtc gccgtggtgg
    18841 gtacatttat gggtctgcta tcgcaacccc tttaatattt tactcaccat tctcggcgct
    18901 atttcttacg ccacggaaga tttatttgct gcgggcgtta tcgcgctaat ggtcgctatt
    18961 tctacgttgc tgaactttat tcaggaagca cgttccacta aagcggcaga tgccctgaaa
    19021 gcgatggtca gcaataccgc gacggtgctg cgcgtaatta acgacaaagg cgaaaatggc
    19081 tggctggaga tcccgatcga ccagctggtg cccggcgata ttataaaact ggcggcgggg
    19141 gatatgatcc cggcagattt acgtatcttg caagcgcggg atctgttcgt cgctcaggcg
    19201 tcgttaaccg gtgagtctct gcccgtggaa aaagccgcta ccactcgcca gccggagcac
    19261 agcaatccgc tggagtgcga cacgctgtgt tttatgggca ccaccgtggt gagcggcacg
    19321 gcacaagcaa tggtgattgc tacaggtgcc aatacctggt ttggtcaact ggcggggcgt
    19381 gttagtgagc aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgtcagtatg
    19441 ctgctgattc gctttatgct ggtgatggca ccggtggtgc tgttaatcaa tggctacacc
    19501 aaaggcgact ggtgggaagc ggcgctgttt gcgctttcgg tcgcggtagg cctaacgccg
    19561 gaaatgttgc cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa
    19621 cagaaagtga tcgtcaaaca tctggatgct attcagaact ttggcgcaat ggatattctg
    19681 tgcactgata aaaccggcac cctgacgcag gataaaattg tgctggagaa tcataccgat
    19741 atctccggta aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattatcag
    19801 accggactta aaaacctgct cgatacagcg gtgctcgaag gtacggatga agagtcagcg
    19861 cgctcgctgg ccagtcgttg gcaaaaaatc gatgagattc cgtttgattt cgagcgtcgc
    19921 cggatgtcgg tggtagtggc agaaaatacc gagcaccatc agctggtttg caaaggtgca
    19981 ttgcaggaaa tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtgccgctc
    20041 gatgacatca tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg
    20101 cgcgtggttg cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg
    20161 gatgaatccg acctgatcct cgaaggatat attgcttttc ttgatccgcc aaaagagaca
    20221 accgctccgg cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc
    20281 gacagtgagt tagttgcagc gaaagtgtgc catgaagtgg ggctggacgc gggagaggtg
    20341 gtcattggta gtgatattga aacgctatct gacgacgaac tggcaaatct tgcacagcgc
    20401 accacgctgt ttgcccgcct gacgccgatg cataaagaac gcattgtgac cttactgaag
    20461 cgcgaagggc atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc
    20521 gcggcggata tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat
    20581 atcatcctgc tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc
    20641 actttcgcca acatgctgaa atacatcaaa atgacggcga gttctaactt tggtaatgtg
    20701 ttcagcgtgc tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg
    20761 cttattcaga acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac
    20821 gacgagcaaa ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatgatc
    20881 ttcttcggac cgatcagctc gatcttcgat attctgacgt tttgcctgat gtggtgggta
    20941 ttccatgcca acacgccgga aacgcaaacg ctgttccagt cgggatggtt tgtggtgggc
    21001 ttactgtcgc aaacgctgat tgtgcatatg atccgcaccc gccgtgtgcc gtttattcag
    21061 agctgtgcat cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca
    21121 ttgccgtttt caccgctggc cagttatctg caattacagg cgttgccgtt aagctatttc
    21181 ccgtggctgg ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaaggattt
    21241 tatagccgtc gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga
    21301 ttgcagacaa agtgagtttt gttcatgccg gatgcggcgt aaacgcctaa tccggcctac
    21361 aaattcaatc aattgcagga atcacgtagg cctgataagc gtagcgcatc aggcgatttt
    21421 gcgtttgtca tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac
    21481 gaatagcgat cgcttcgatc tcaatcttta cgtctttcgg cagacgggca acttcaacgc
    21541 aagaacgtgc cgggaaggtg gcgttgtgtt cggtgaagaa ggtttcgtaa gtggcgttta
    21601 cggttgcgaa gtcgttcaga tcttttacaa acacggtagt tttaacgatg tcgcccactt
    21661 tcagaccagc ggcttcgacg atcgctttta cgttatccag cgactgacgt gcctgtgcag
    21721 cgacgtctgc cggtacttcg cccgttttcg gatttaccgg gatctgaccg gaggtgatga
    21781 tcatatttcc cagatcaacg ccctgtacgt aaggaccgat agctgctggt gcattttccg
    21841 tcgcgatagt tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta
    21901 tagggagcca gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa
    21961 actctttttc acagtatttg catttgagcg cgatgtcatt ggcgcgtttt cgcacggcaa
    22021 agctggatga aaccggttcg gcatggctga tacagttgct gttcgggcag accaacacat
    22081 tgtcgatgcg ctctggcaga ctggggcgcg atttacccac cacttcatag ttgtcgatac
    22141 ggttaaccgt ggcttgcggc gcatacaatg ccagttgatc tacctgatct tcactcaaaa
    22201 aggtattttc gattttgatc agatctttgc ggcccatctc gccagaaggc aggttcagac
    22261 caatggtgat gcgctgatcc gtttcggtca gcttgaacag actcaacagc ttaaaaccga
    22321 tctgggcggg gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat
    22381 tatcgtgtgt catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca
    22441 gtaacgcctg gcgagcgaaa atcccgttgc ctgcctgctg gaagtaccag gcgtgtggcg
    22501 ttttatcaac atccgtcgca atttcatcaa cacgcggcag cggatgcagt actttcatat
    22561 tggctttggc gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt
    22621 attcggatgg gtccaggcgc tctttttgca cgcgggtcat atacaggatg tctacttccg
    22681 ccatcacttc ttcaatagag ctgtgcagac tccatgcgat ccctttttca tcgagcatat
    22741 ccagaatgta ttgcggcatt gccagcgcgt ccggcgcgat gaagtaaaaa cggttgccgt
    22801 cgaacttcgc taacgcctga gtcagggagt gaacggtgcg gccatatttc aggtcaccaa
    22861 ccattgcgac gtggagattg tccagacgcc cctgggtttc ctgaatagtg aataagtcca
    22921 gcaaggtttg cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc
    22981 cggaaaactc ggtggccagg cgcgccgcac cttcctgcgg atgacgcatc actatcgcat
    23041 cgacgtaagt gctgataacc gaaatggtat cggccagcgt ttcgcccttt ttacccagtg
    23101 atgtattggc gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt
    23161 cgaaagagag gcgggtacgg gtagaggctt cgaagaagca gctggcaatg actttgtgct
    23221 tcaacagctc tggttgcggg tttgctttca gttttgccgc tgttgccagc accagattaa
    23281 ggtcatcgcg actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca
    23341 tcttttatct cctgacgcct gggcaaaaaa aagcccctcg attgaggggc tgggaatggg
    23401 tgatcaacgg gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat
    23461 gtcgaacaca ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatcgtca
    23521 gaagcggtgc aagcaattga tgcaatattt tgtcagcgca aacggtttat ttgaatttaa
    23581 agttaaaata tatgcatttt tatttactgt gattctgggg ggaactgtcg ttcggtatca
    23641 ataattgcag acatttcctg cttttttctt tttcactttc acgcaatcag atatgcattt
    23701 tattccattc atccgactta tagggcggag tgtgaaaacg aacggctagc actattgctt
    23761 agagctcagg gatgcgcgct atcactttaa tttcaaaatc aaagcctgcc agccatgtaa
    23821 cgcccaccgc cgtccaggtt ggataagatg gcgcgctaaa tatttcattt ttcaccgtca
    23881 tgatgtcttc aaattgtttt tctggatcgg tatgaaagct cgtaacatca atgatatcgt
    23941 caaaagtgca tcccgcagct gccagggtcg catgcaaatt atcaaaggcc agtctgactt
    24001 gttgctgaaa atcgggttct ggtgttccgt cctctcgact tcctacttgc ccggaaacaa
    24061 acagcaaatc gccggaacga atagccgcag aataacgatg ctcagcatac agtgaatgtc
    24121 gtccagcagg gaaaacagcg gttctttcta ccatttggtt atcctcaaga tttacgacat
    24181 gaacagaaga tttatattta ccaggagccg cttttagcgg acgacgtgag taaacaaaac
    24241 ccagacatca tgggaaatgg ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg
    24301 ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc taaccatgcg
    24361 accataccag cgacctcttc cggttgccca tgtcttttga tggccataaa actatgcgac
    24421 atatcgcgca ttggcccgtt ggcgggatta gcgtcggtat caattggccc tggctggacg
    24481 acgttgatgg tgatcccacg cggtccgaaa tcacgggcca gcccgcgcgc catgccttgc
    24541 agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg atcgccattc
    24601 acggagccga tgattaagat gcgtccgcct tcgggcatct gccgggcggc ttcaacagag
    24661 gcatggtaag gagcatgaat attgattttg aaaaggcgat caatatcgtc ggcatttaat
    24721 tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc caatgcgccg
    24781 ctcttacgaa cgacatcaat aacagcgtct ttgtcagcac tatctgtgaa tactgctgtc
    24841 gctccagtct cttgtgccag gcgttcagcg gcatctttcg accccgcata ggtgaatcgt
    24901 acattggccc catcggtgac gaaacgacgt acgatagcgg caccgattcc acggctacca
    24961 ccgaggatga gaactgtctt acctgtaaaa gcgcccataa ggactccttg atttattatg
    25021 taacatgcat tacaaaactg ttttaacttt ctgtcaacaa gttttgtaat ggtcactaaa
    25081 aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg
    25141 gcaaaagtgc tttttcacca aaaaggtttc gatgctgtca gtgttgctga agttactgat
    25201 tatcttggta ttaacccccc gagcctctac gcggcttttg gcaataaggc tgggttattt
    25261 agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga tattcttcgt
    25321 gatgatcgtc cggtagggga gtgcctggct gaggtattaa aagaagcggc gcgccgatat
    25381 agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gcattcatag tcatgatcca
    25441 caagcgcgtg atattgccgt tcaatattat cacgccgcag aaacgaccat ttatgactat
    25501 atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag taccgtgatg
    25561 tcagggcttt ctgcgaaggc acgggagggg cactccatcg aacaactctg tgcaacagct
    25621 gcactggcgg gggaagcgat aaaaactatt ctcaaggagt gatgcgggcc ttgacccgaa
    25681 aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgcg ttaaggctag
    25741 cggaattgat catcttttcg tataacgatg gaaatgaaac gttgttttaa ttaaggagtg
    25801 gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca ggagttacgc
    25861 caggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg caagcacgat
    25921 atcgaaggca atcgtctgtt ttatcttatc tcggaagata tgaccgagcc gcacgaagct
    25981 cgccgtgcgg agtaccatgc ccgctatctc gacattcaga ttgtgttaaa aggtcaggaa
    26041 ggcatgacct tcagcacgca acctgcaggc accccggata ccgactggtt agctgataaa
    26101 gacatcgcat ttttgccgga aggcgttgat gagaaaacag ttatccttaa tgaaggtgat
    26161 tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg cgcgccagcc
    26221 cgggttcgca aagcagtagt gaagatgctg atggcgtgat gacttttcgc cgtaaataac
    26281 tccaggttta cggcgagttt gtgaaaagag cgttttttga tatttttttg tgagtaaaat
    26341 ttgtaatgct tagacgttct tattaactca aggagttcgt catgagcaaa atatcagatt
    26401 tgaattattc tcaacacatt acattagccg acaattttaa acaaaaaagt gaagttttaa
    26461 atacctggcg tgttggaatg aataattttg cccgtaatgc cgaggggcag gataacacaa
    26521 gaaatatcct tgatcctaag acatttttgg aatttttggt aaaaatattt actctaggtt
    26581 atgtggattt tagcaaacgc tccaacgaag cgggaagaaa tatgatggct catattgagt
    26641 cctcatctta tatcaaaaat aatgatggca gtgagataat aaagtttgtt atgaataatc
    26701 ctgaagggga acgagcggat ttatctaagg tggaaataga aatcacactt tccactttca
    26761 ctactatggg gactcgtcaa ggacatacgg ctattatatt tcaacaacct gatggttcga
    26821 ctaatcgtta tgaaggaaag tcctttgaaa gaaaagatga gagttcatta cacctgatta
    26881 ctaacaagat tctggcatgt taccaacgcg aagctaacaa ggaaatagct cgtctattaa
    26941 atatttcaca ggagttaaat aattctcagg acttgaataa ctcgcaggtg agttgtaaag
    27001 attcagttga ttctacgatt acggatttat tagaaaaacc attgaataat gcattattag
    27061 caataaggaa agaacatctg ctattaatgc cttatgtatg taatgaatcg atttcatact
    27121 tgcttggtga aaaagggata ctgaaagaaa tagatgacct taacgcagta aataattatc
    27181 ttcttaataa taagaaagct actgacaatg aaattaatga catcaaagtt aacctgtctc
    27241 atattcttat tgattcctta gatgatgcaa aagttaacct tacaccggtc atcgattctg
    27301 gagacttttt taaaatcccc atatattaat gatgtaagaa tactggattg gtgttttaat
    27361 aaaagaatgc aatattttgg tgattctgaa aagataaagt atgcatgctc cgtgataaat
    27421 catattgatt ttagccgcga tcaatctaaa gattttagct gcgatcaatc taaaattaaa
    27481 atagctgaga ccttattttt caatctcgat aaagaacc
//
LOCUS       DAJTNN010000037        27206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTNN010000037 DAJTNN010000000
VERSION     DAJTNN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.037"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(40..1338)
                     /gene="eno"
                     /locus_tag="OO150_001407"
     CDS             complement(40..1338)
                     /gene="eno"
                     /locus_tag="OO150_001407"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5515602.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1426..3063)
                     /gene="pyrG"
                     /locus_tag="OO150_001408"
     CDS             complement(1426..3063)
                     /gene="pyrG"
                     /locus_tag="OO150_001408"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5515603.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3291..4082)
                     /gene="mazG"
                     /locus_tag="OO150_001409"
     CDS             complement(3291..4082)
                     /gene="mazG"
                     /locus_tag="OO150_001409"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5515604.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4153..4488)
                     /gene="mazF"
                     /locus_tag="OO150_001410"
     CDS             complement(4153..4488)
                     /gene="mazF"
                     /locus_tag="OO150_001410"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR5515605.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4488..4736)
                     /gene="mazE"
                     /locus_tag="OO150_001411"
     CDS             complement(4488..4736)
                     /gene="mazE"
                     /locus_tag="OO150_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR5515606.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4814..7048)
                     /locus_tag="OO150_001412"
     CDS             complement(4814..7048)
                     /locus_tag="OO150_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5515607.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7096..8397)
                     /gene="rlmD"
                     /locus_tag="OO150_001413"
     CDS             complement(7096..8397)
                     /gene="rlmD"
                     /locus_tag="OO150_001413"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5515608.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8454..11210
                     /gene="barA"
                     /locus_tag="OO150_001414"
     CDS             8454..11210
                     /gene="barA"
                     /locus_tag="OO150_001414"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5515609.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11442..12782)
                     /gene="gudD"
                     /locus_tag="OO150_001415"
     CDS             complement(11442..12782)
                     /gene="gudD"
                     /locus_tag="OO150_001415"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5515610.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDNAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12803..14143)
                     /locus_tag="OO150_001416"
     CDS             complement(12803..14143)
                     /locus_tag="OO150_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5515611.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELHVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14145..15515)
                     /gene="gudP"
                     /locus_tag="OO150_001417"
     CDS             complement(14145..15515)
                     /gene="gudP"
                     /locus_tag="OO150_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5515612.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(15932..16381)
                     /locus_tag="OO150_001418"
     CDS             complement(15932..16381)
                     /locus_tag="OO150_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5515613.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16399..17181)
                     /gene="truC"
                     /locus_tag="OO150_001419"
     CDS             complement(16399..17181)
                     /gene="truC"
                     /locus_tag="OO150_001419"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5515614.1"
                     /translation="MLEILYQDEWLVSVNKPSGWLVHRSWLDRDEKVVVMQTVRNQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17181..17510)
                     /locus_tag="OO150_001420"
     CDS             complement(17181..17510)
                     /locus_tag="OO150_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5515615.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(18132..18677)
                     /gene="syd"
                     /locus_tag="OO150_001421"
     CDS             complement(18132..18677)
                     /gene="syd"
                     /locus_tag="OO150_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5515616.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18745..19593
                     /gene="queF"
                     /locus_tag="OO150_001422"
     CDS             18745..19593
                     /gene="queF"
                     /locus_tag="OO150_001422"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5515617.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPKVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19705..21069
                     /gene="ppnN"
                     /locus_tag="OO150_001423"
                     /gene_synonym="ygdH"
     CDS             19705..21069
                     /gene="ppnN"
                     /locus_tag="OO150_001423"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5515618.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKAVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21626..22915
                     /gene="sdaC"
                     /locus_tag="OO150_001424"
     CDS             21626..22915
                     /gene="sdaC"
                     /locus_tag="OO150_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5515619.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22973..24340
                     /gene="sdaB"
                     /locus_tag="OO150_001425"
     CDS             22973..24340
                     /gene="sdaB"
                     /locus_tag="OO150_001425"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5515620.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24452..25207
                     /gene="xni"
                     /locus_tag="OO150_001426"
     CDS             24452..25207
                     /gene="xni"
                     /locus_tag="OO150_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5515621.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25312..26449)
                     /gene="fucO"
                     /locus_tag="OO150_001427"
                     /pseudo
     CDS             complement(25312..26449)
                     /gene="fucO"
                     /locus_tag="OO150_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26477..27124)
                     /gene="fucA"
                     /locus_tag="OO150_001428"
     CDS             complement(26477..27124)
                     /gene="fucA"
                     /locus_tag="OO150_001428"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5515622.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIAFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6577 a         7297 c         6556 g         6776 t
ORIGIN      
        1 tcccggaggc tggcattttt aaatcagata aagtcagtct tatgcctggc ctttgatctc
       61 tttacgaccg ttgtacggtg ctttttcgcc cagagcttct tcgatacgaa tcagctggtt
      121 gtatttagca acacggtcag aacggctcat agaaccagtt ttgatctggc ctgcagcagt
      181 accaacagcc aggtcagcga tggtagcgtc ttcagtttcg ccagaacggt gagagataac
      241 tgcagtgtag ccagcatctt tcgccatctt gattgcagcc agagtttcgg tcagagaacc
      301 gatctggttg aatttgatca ggatggagtt agcgatacct ttttcgatac cttctttcag
      361 gatcttggtg ttggttacga acaggtcgtc accaaccagc tggattttgt cgcccagaac
      421 tttggtctgg tatgcgaaac cgtcccagtc agattcgtcc agaccgtctt cgatagaaac
      481 gatcgggtac tgtttggtca gttcttccag gaagtgagtg aattcttcag aggtgaacgc
      541 tttgttgcct tcgccagcca gaacgtattt accatctttg tagaattcag aagctgcgca
      601 gtccatcgcc agagtgatgt ctttgcccag ttcataacca gcagctttaa cagcttcagc
      661 gataacagcc agagcttcag cgttggaacc caggttcggc gcatagccac cttcgtcacc
      721 aacagcagtg ttcatgcctt tcgctttcag aacttttgcc aggtgatgga aaacttcaga
      781 acccatgcgg atggcttctt tcacagtttt cgcgccaacc ggctgaatca tgaattcctg
      841 gatatcaacg ttgttgtcag cgtgctcacc accgttgatg atgttcatca tcggaaccgg
      901 catagagtat ttgcccggag taccgttcag ttcagcgatg tgctcgtaca gcggcatacc
      961 tttagcagct gcagcagctt tggcgttagc cagagataca gccaggattg cgttcgcgcc
     1021 gaatttggat ttgttttcgg tgccgtccag gtcgatcatg atcttgtcaa tgccagcctg
     1081 atctttagca tctttgccaa tcagcgcctg agcgatcggg ccgtttaccg cagcaacagc
     1141 tttggttacg cctttaccca ggaaacggga tttgtcgcca tcgcgcagtt ccagagcttc
     1201 acgggaacca gtagaagcac ctgacggagc agctgccata ccgacgaaac caccctccag
     1261 atgtacttcg gcttcaacag tcgggttacc acgggagtcg atgatttcac gaccgatgat
     1321 ttttacgatt ttggacatta ggttttcctc aagtcactag ttaaactgaa actccagaca
     1381 aacaacacgt acccagggta cgcgttgccg ttctaacttt tttacttact tcgcctgacg
     1441 tttctggaac tcgctggcgg ctttcacaaa gcctgcaaac agcgggtgac catcacgtgg
     1501 agtagaagta aactccggat ggaactggca agccacgaac cacgggtgat tcggaacttc
     1561 gatgatctcg accaactgat catccccgga acggcccgca acgcgcagac ctgcatcttc
     1621 aatctgtttc aacagcatgt tgttgacttc gtaacggtga cgatgacgct caacaattgt
     1681 cggcgcattg tacagctggc gaaccaggct atcgtcaacc aactggcact gctgtgcgcc
     1741 gagacgcatg gtaccgccga gatcgctctt ctcgctacga acttcaacgt tgccgttttc
     1801 atcgcgccac tcggtaatca gcgccacaac cgggtactta cagtctggca caaattccgt
     1861 agagttggcg ttctccatgt tggcaacatg gcgagcgtaa tcaattaacg ccacctgcat
     1921 acccaggcaa atgcccagat aaggaatatt gttctcacgc gcaaaacgcg cggtcgtaat
     1981 catgccttct acgccacgat agccgaaacc gccaggtacg aggattgcgt ccagaccttt
     2041 aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg atcagtttga tgttgacgct
     2101 gacacgattc ttcagcccac cgtgtttcag tgcttcgatc actgatttat aagcatccgg
     2161 cagttcaatg tacttgccga ccataccgat ggtgacttca cttaccgggt tcgcttcttc
     2221 gaagataacc tgttcccatt cggacagatt cgcttccggg cagtttaagc tgaatcgttt
     2281 acaaatataa tcgtccagcc cctgagattt caacaggccc ggaattttat agatggaatc
     2341 gacgtctttc agagaaataa ccgctttttc cggaacatta cagaacaatg caatcttcgc
     2401 acgttcgttc gccggaacag cgcgatctga acgacaaatc aggatgtcag gctggatacc
     2461 gatggagagc agctctttta cagagtgctg agtcggtttg gttttgactt caccagacgc
     2521 tgccatgtac ggcaccagcg tcaggtgcat aaacagagtg tgctcacggc caatttcaac
     2581 agccatctgg cgaatcgctt cgaggaacgg caaggattcg atatcaccta ctgtaccgcc
     2641 gatttctacc agtactacgt catgaccttc gccaccttcc agcacgcgct ctttgattgc
     2701 gttagtgatg tgcggaataa cctgcacggt tgcgccgagg tagtcaccgc ggcgttcttt
     2761 acgcagaacg tcagagtaga tacgacccgt ggtgaagttg ttgcggcggc tcattttggt
     2821 acgaatgaaa cgctcgtagt gccccaggtc caggtcggtt tcagcgccgt cttcagtaac
     2881 gaacacttcc ccgtgttgga ttgggctcat agtacctgga tcgacgttga tgtacggatc
     2941 cagtttcatg atggtcacat tgaggccacg ggcttcaaga atggctgcga gggaggctgc
     3001 ggcaatgcct ttacccagag aggatacgac cccgccggtc acaaaaatat agttcgttgt
     3061 catgctgaac ctgagaagtt aggttgaaaa gacgatggaa taaccaggac gggaaagcag
     3121 tatacccgaa catgacctgt gccacaaact ttcattatcc ctcctcttcg ccagcgcact
     3181 attgaaatca ggagtgagaa aatagcccct ttggggtaaa tgtttttgac gcaaatcaag
     3241 cgcttgtcat ttaaaaaatg acacaaatgg cacttgaccg cgtaattccc ttagagatca
     3301 atttcctgcc gttttacctg ttgccagact tcttccattg tttcgaggtc aacacctgtc
     3361 atttccagtc cacgcgcggc aacaatacgc tccacttcgc gaaaacgacg ctcgaatttt
     3421 tcgttcgctt tttgcaatgc gatttctgct ttcgtcccta aatggcgagc cagattaacc
     3481 gtggcaaaca gcaggtcccc catttcctcc tccagtttag cctggtcgac aacagcctgc
     3541 cgcgcttcgt acatcacctc gtcgatctct tcgtagactt tatcaaccac cgggccaagc
     3601 gtcgtccagt cgaagccaac gttggcgcaa cgtttctgga ttttttgcgc ccgcattaaa
     3661 gccggtaaac tacgaggaat atcgtccagc gccgaatgct gcgctttctg cgcgcgctct
     3721 tcggttttga tttgctccca acgggcaagc acttcactac tgttttcggc agaactatca
     3781 gcaaaaacat gcggatggcg acgctctaat ttatcgctaa tagcagcgca aatatcatta
     3841 aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa acaccacctg gaatagcaga
     3901 tcgcccagtt caccgcgaag atcgtcaaaa tcttcacggg cgatggcgtc cagcacttcg
     3961 taggtttctt caagggtgta aggcgcaatg gtggcaaatg tctgctcttt atcccacggg
     4021 cagccgtttt ccggatcgcg caggcgctgc ataatagtga gcaaacgatc gatttgattc
     4081 attgaattgt cctgaaaatt gcgggtctgt caggtggaaa cctgtgacca gaatagaagt
     4141 gagttagtaa cactacccaa tcagtacgtt aattttggct ttaatgagtt gtaattcctc
     4201 tggggcaact gttcctttct tcgttgctcc tcttgcccgc caggcgattc tttttacctg
     4261 atcagctaac gctacgccat cacgttcctg accggataaa acaacttcga acggatatcc
     4321 ttttgattgc gttgtacaag gaacacacag acacatacct gttttgttgt tgtacatgaa
     4381 aggactcagg acaacagctg gacgatgtcc agcttgctcg ctaccttttg tcgggtcaaa
     4441 atcaacccaa atcagatcgc ccatatcggg tacgtatcgg cttaccatta ccagacttcc
     4501 ttatctttcg gctctcccca gtcgatattc tcgtggaggt tttccggcgt gatgtcgttg
     4561 accagttcag caagcgtaaa tacgggctct ttacgcactg gctcaataat taatttgcca
     4621 tccaccaggt caatcttcac ttcatcatca atattcagat tgagcgcctg cattaacgta
     4681 gccgggatcc gcaccgccgg tgaatttccc caacgcttta cgctactgtg gatcatcacc
     4741 ctttcctcaa accgctatca tatgtagata cagtatatat caatctacat tgtagatacg
     4801 agcaaatttc tgcctaactc ccgtgcaacc gacgcgcgtc gataacatcc ggcacctggt
     4861 tgagtttacc cagcacgcgg cccagcacct gcaggttgta aatctcaatg gtcatgtcga
     4921 tggtcgccag ttgctgtttg gtgtcgctac ggctggcaac gccaagtacg ttcaccttct
     4981 cgttggcgag aatggtcgtg atatcacgta acaacccact acgatcatta gccaccacgc
     5041 ggaccaccag cgaatatccg gcggagtagc tctcacccca taccgcgtca acaatgcgtt
     5101 ctggcgcatg agagcgcagt tccgccagtt gttcgcaatc ggcgcggtgt actgaaatac
     5161 cgcgcccctg ggtaatgaag ccgacaatct catctccagg aatcggctgg cagcagcgcg
     5221 cgatgtggtg catcaggttg ccaacacctt cgactaccac gcgaccgtta tctttactgc
     5281 ggttttgcgg cgtgtagctt ttttgctgaa gttgcttcag cgcggcggcg tcctgctctt
     5341 cggcactcgg cttattaaat tgcgattgca ggaagttcac catctgattg agacggatat
     5401 ccccgccacc aatcgccgcc agcaactcgt cgacatcatt aaagttgtaa cgcggcagca
     5461 gatgtttttc tgcttctttc aggctgatcc ccagatgttc cagctcgtcg tcaaggattt
     5521 gccgcccagc cagaatgttt ttgtcacggt cctgtttacg gaaccaggcg tgaattttcg
     5581 aacgcccacg gctggttgtg acgtaaccga ggtttgggtt taaccagtca cggctggggt
     5641 tcggctgttt ctgggtgata atttcaatct ggtcgcccat ctgcagctgg taggtgaacg
     5701 gcacaatgcg cccgccaatt tttgccccga tgcagcggtg tccgacatca ctgtggatgt
     5761 ggtaagcgaa gtccagcggc gttgatcccg caggcaaatc aacgacatca cctttcggcg
     5821 taaagacata cacccggtcg tcaaagacct ggctgcgtac ttcgtcgagc atttcgccgg
     5881 aatcagccat ctcttcctgc cacgcaatca gtttacgcag ccaggcaatc cggtcttcat
     5941 gtcccgaacg tgcgccgcca gctgccgcgc cctctttata tttccagtgc gcagcaacac
     6001 ccaactctgc atcttcatgc atctgtttgg tgcggatttg gatctcaacg gttttgccac
     6061 ctggccccag aaccacggta tgaatagact gataaccgtt tggtttcggg ttagcgacat
     6121 aatcgtcaaa ctcatccggc aggtggcgat agtgagtgtg cactatcccc agtgcggcat
     6181 aacaatcctg taaacgctcg gcgacaatac gtaccgcacg cacatcaaac agctcatcga
     6241 aggcgaggtt ctttttctgc attttgcgcc agatgctgta gatgtgtttc ggacggccat
     6301 acacttcagc tttaacgcct tcagctttca tctcagcgcg cagatgaccg acaaactctt
     6361 cgatatagtg ttcgcggtcg agacggcgtt catgcagcag ttttgcgatg cgtttgtatt
     6421 cggtcgggtg cagataacgg aagcagtaat cttccagctc ccatttcagt tgcccaatcc
     6481 caagacggtt cgccaacggc gcgtagatat tggtgcactc ttttgccgcc agtacgcgtt
     6541 catcttccgg cgcatctttt acttcgcgca gatgagcaat acgctccgcc agtttgatga
     6601 ccacgcagcg aaaatcatcg accatcgcca ataacatccg gcgaacgtta tcgacctgtt
     6661 cggaggaaac agaatcagtg tgcgtcgctt tcagctggcg gatcgccgcc atatcacgca
     6721 cgccgtgaat aaggttaacg acagacttac cgacgctctc acgcagcaca tcttcgctga
     6781 ctacgttggc atcggccaga gggaacagca gcgccgcccg cagcgtgtca atgtccatac
     6841 ttaatgtcga gaggatctcc accatctcaa caccacgcca caataacaga ctggcatccg
     6901 gatgcccctg cgtctgctgc agacaatacg cccaggtttc ggctaagcac tcacacgact
     6961 tctggctggt aatacccaga cttgcgatcc atttttccgg atcaaattca ccagccttat
     7021 tgatatgtgc acttcttacc gcaaccattg tcctctcctt tagggaccag acctgccgaa
     7081 atcggcaaat cgtaactatt taacgcgcga gaaaagtacc atcgattcca gatgtcccgt
     7141 gtgtgggaac atatccagca tcgccagtcg cgcaatggta tatcctgctt ttaataacgc
     7201 ttcgctatcc cgagccagcg ttgcagggtt acaggataca taaactatac gaataggttc
     7261 cagttttata atttgctgca taacacctgc ggcacctgct cgcgccgggt ccagcaacac
     7321 tttatcgaag ccgtttttcg cccacggctg ctttgtgaca tcttcttcaa gattttcgtg
     7381 ataaaacgtc acattctgta agccattaag acgcgcattc tgctggcctt tttccaccag
     7441 cgccggaaca ccttctacac cgaccacact ggcagcttgt gtcgccaatg gcagtgtaaa
     7501 gttgcccata ccgcagaaca gatccagtac gcgatcttca ggttgcacat ccagccattc
     7561 caacgcactc gctaccattt tttggttcac acccgcattg acctgaataa aatcgcgcgg
     7621 gctaaaagtt aagcgcaacc cgttagagtc ataccagggc atctcaccag agacggtttc
     7681 gagtatctca ctatcggggg cgagatacag atccaggcct tcagaatgcg aaaagcgttc
     7741 cagtttttcg cgatctgccg aacttagcgg tgcggtatgg cgcaaaatca tcagcgtgcc
     7801 gctggttgcc tgtaccagtt caacatgacc aagatggcgc attgcttgta agctgcccag
     7861 gcatgccctg actttgggca gcaatgcttc aagttggggc actaaaatgg gacattgttt
     7921 aacgtcgaca atgtcactgg agcccgcttt gcgaaacccc atctgaagtt gctgtgtttt
     7981 cggtaagtag ttcagactta aacgcgcgcg acggcgatag ccccagggaa catcggcgat
     8041 cacttcagag acttcgtgtt tcattaatcg ggcgagtgcc gcacttttgc ttcgctgctg
     8101 taaatccacg ctggcgtgtt gttgctgaca gccgccgcat acgccaaaat gaggacagcg
     8161 tggcgtttcg cgttccgggc tatcgcttaa ccggcgtacg actttagcgc gggcatactg
     8221 ttttttatct tcagtaacag taacttccgc gttttcctgc ggcaataatc cggggataaa
     8281 tagcgttttg ccgttatgtc gcgccacgcc ctgaccaaaa gagtcgaggt cgttgactga
     8341 aacggttatg atctgacgcg tcgtcgtgcg tcgttttgca gagtagaatt gcgccattgg
     8401 cgagactttc tcaatttaac agtgtgacct taattgtccc ataacggaac tccatgacca
     8461 actacagcct gcgcgcacgc atgatgattc tgatcctggc accgaccgtc cttattggtt
     8521 tattgctgag tatatttttc gtcgtgcatc gctataacga cttgcagcgt caactggaag
     8581 atgcaggtgc cagcattatt gagccgcttg cagtttctac tgaatatggc atgagcctgc
     8641 aaaatcgcga atctatcggt cagttaataa gcgtactgca tcgtcgccat tccgatattg
     8701 ttcgcgcgat ttcggtttat gatgaaaata accgactctt tgtcacctcc aattttcatc
     8761 ttgatccctc atcaatgcag ctcggcagca acgtgccgtt tcctcgccag ctcactgtca
     8821 ctcgtgacgg cgatattatg atcctccgca cgccgattat ttctgagagt tactcccccg
     8881 acgaatcgcc cagtagcgat gccaaaaata gtcaaaatat gttgggatat attgcgcttg
     8941 agctggatct taaatcggtt cgcttgcagc aatataaaga gatctttatt tccagcgtga
     9001 tgatgctgtt ttgtatcggt attgcgctta tttttggctg gcgcttaatg cgcgatgtaa
     9061 ccggtccgat tcgcaacatg gtgaataccg tcgaccgcat ccgtcgcggg caactcgaca
     9121 gccgggtgga aggatttatg ctcggcgagc tggatatgct gaaaaacggt atcaactcga
     9181 tggcaatgtc gctggctgct tatcacgaag agatgcagca caatatcgac caggcgacgt
     9241 ccgatctgcg tgaaacgctg gagcagatgg aaattcagaa cgttgagttg gatctggcga
     9301 aaaagcgcgc ccaggaagcg gcgcgtatta aatccgagtt tctggcaaat atgtcacacg
     9361 agctgcgtac accactgaat ggtgttattg gctttacccg cctgacgctg aaaacagaat
     9421 taacaccaac gcagcgcgat cacctgaata cgattgaacg ttcggcaaat aatttgctgg
     9481 caattattaa tgatgttctc gacttctcga aactggaagc aggtaagctg attctggaaa
     9541 gtattccatt cccactacgc agcacgctgg atgaagtcgt tactctgctg gcacattctt
     9601 ctcacgataa agggttggaa ctgacgctca atattaaaag cgacgtgcct gataacgtga
     9661 tcggcgaccc actgcgatta cagcaaatca tcactaacct ggtggggaat gcaattaaat
     9721 tcaccgagaa tggcaacatt gatattctgg tagaaaaacg tgcgctgagt aataccaaag
     9781 tgcagattga agtgcagatt cgggataccg gcattggtat tcctgaacgc gatcaatcgc
     9841 gcttattcca ggccttccga caggctgatg ccagtatttc ccgccgtcat ggtggcaccg
     9901 ggctggggct ggtgattaca caaaaactgg ttaatgaaat gggcggcgat atttcgttcc
     9961 atagccagcc gaatcgcggt tcaactttct ggttccacat taatctcgat ctgaacccga
    10021 acattattat cgaagggcca tccacccagt gcctcgcagg taagcgcctg gcctatgtcg
    10081 aaccaaactc cgcagcagcg caatgcacgc tggatatttt aagtgaaacg ccgctggaag
    10141 tggtttatag cccaacgttc tccgcgctgc ctcccgcgca ttacgacatg atgttgttag
    10201 gcatcgcggt gaccttccgc gagccgctaa caatgcaaca tgagcgatta gcgaaagcgg
    10261 tatcgatgac cgatttcctg atgctggcac ttccttgcca tgcacaagtc aatgctgaaa
    10321 aactcaagca agatggtatc ggcgcgtgtc tgctgaaacc attaacacct acacgcctgt
    10381 tgcctgccct gacggaattt tgtcatcaca aacaaaacac gcttttgcct gtaaccgatg
    10441 aaagtaagct ggcaatgaca gtcatggcgg ttgatgacaa ccccgctaac ctgaaactta
    10501 tcggcgcatt gctggaagat atggtgcaac atgtggaact ttgcgatagc gggcatcagg
    10561 cggttgaacg ggcgaaacag atgccgttcg atttgatctt aatggatatt caaatgccgg
    10621 acatggatgg cattcgggcc tgcgaactca tccaccagct cccccatcag caacaaacgc
    10681 cggttatcgc ggtaacggcg catgcaatgg ccgggcaaaa agagaagctg cttggcgcag
    10741 ggatgagcga ttatctggcg aaaccgattg aagaagagcg attgcataat ttgttgttgc
    10801 gctacaagcc tggcagcggt atttcctctc gcgtcgtgac gcccgaagtc aacgaaattg
    10861 tggtgaaccc gaatgcgacc ctcgactggc aactggcact acgccaggca gcaggaaaaa
    10921 ccgatttagc gcgcgatatg ctgcaaatgt tactcgattt cctgcctgaa gttcgcaaca
    10981 aagttgagga acagctggtt ggagaaaacc cggaaggcct ggttgatttg attcataaac
    11041 tgcatggcag ttgcggctat agcggtgtgc cgcgtatgaa gaatctctgc caactgatcg
    11101 aacaacagct acgtagcggt actaaagaag aagatttgga gccggagctg ctggaactgt
    11161 tggacgagat ggataatgtc gcgcgcgaag ccagcaaaat tctcgggtaa cggacgtctt
    11221 gtcgagtagc aatcggcttt ttatgacgct ggtttcagat tggtgacaaa gtgcctgtgt
    11281 ttatgccgga tgcggcgtta acgccttatc cggcctacaa aaccaattaa attcaatgaa
    11341 ttaaaaaatg atgtagacct gatacatagc acaacaggct attttgcgtt tagcatcagt
    11401 ctcaagccgg ctccagatag agccggtttt ggttttctgt cttaacgcac catgcacggg
    11461 cgcttgttat cgaacgtcca gccaggaatc agatactgca ttcccatcgc attgtcacgc
    11521 gcgcaaagcc cgtgtttctg atacagctca tgggctttca tcacttgatc catatcgatt
    11581 tctacaccca gccccggttt ttccggcacc tgaaccagac cgcctttgat ctcaaacggt
    11641 tctttggtca agcgctgatt gccttcctgc caaatccagt gcgtatcaat cgcggtgatt
    11701 ttacccggtg cagcggcggc aacatgggta aacatcgcca gggaaatatc gaagtggttg
    11761 tttgagtgtg aaccccaggt caggccaaat tcatggcaca tttgcgccac acgtaccgaa
    11821 ccttgcatcg tccagaaatg cggatccgcc agcgggatat caacggattg cagggagagc
    11881 gtatggccca tctgccgcca gtcggtggcg atcatattgg ttgcggtcgg caggccagtt
    11941 gcgcgacgga actctgccat cacttcacgc ccggagaaac cttgctccgc accacacgga
    12001 tcttctgcat aagccagcga ccctttcagg tatttaccaa ttttaatcgc ttcgttcagc
    12061 gaccaggcac cgttaggatc gagcgtaata cgcgcctgcg ggaagcgttg cgccagtgcc
    12121 acaatagact cggcctcttc ttccccggcc agtacaccgc ctttcagttt gaaatcgttg
    12181 aagccatatt tttcatacgc cgcttccgcc aggcgcacca ccgcatccgg cgtcatcgct
    12241 tcttcatgac gcaggcgata ccagtcgcat gagtcatccg gctggctttg atacggcagc
    12301 ggtgtggctt tgcgatcacc gacgaagaac agataaccga gcatttcgac ttcgctacgc
    12361 tgttgaccat cgcccagcag ggatgccacg tttaccccca gatgctgccc cagcagatcc
    12421 agcattgccg cttctatccc ggtaactaca tgaatagtgg tacgcaaatc aaatgtctgc
    12481 aaaccgcgcc caccagcatc acgatcggca aaagtattac gcaccagcgt cagaacgttt
    12541 ttgtattcac ccagcgtttt acctaccacc agcggaatcg catcttccag cgttttacgg
    12601 attttctcgc cgccgggaat ttcccctacg ccagtgtgac cagaattatc tttgataatc
    12661 ataatattac gcgtaaagaa cggtgcgtgt gcaccactca gattcatcag catactgtca
    12721 tgacccgcca ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa ttgagaactc
    12781 atatttatgt cctttttcag aatcaatgac ggccgaaaac gggacgttta cggtcaaagg
    12841 tccagccggg gatcaggtac tgcatcggac ctgcgtcgtt acgcgcaccg ccaggcagac
    12901 gtttataggc ctcatgtgcc ttttgtacct gttcccagtc cagttccacg cccagaccgg
    12961 gcgcatcagg aacggcaatt tttccgtttt taatctccag cggatttttg gtcaggcgac
    13021 aatcgccctc ctgccaaatc cagtgggtat cgatagcggt aggattaccc ggtgccgccg
    13081 cgcccacatg ggtaaacatc gccagagaga tatcgaaatg gttattagaa tggcagcccc
    13141 aggtcagccc ccagtcgtcg caaagctgcg ccacacggac tgcaccggaa agcgtccaga
    13201 agtgcggatc ggcaagtgga atatctaccg cattgagcat caccgcatga cccatttcgc
    13261 gccagttggt ggcgatcatg ttagtcgcga cgggcaagcc ggtcgcccgc cgaaactccg
    13321 ccataacttc acgaccggag aaaccctgtt ctgcgccgca tggatcttcg gcataggtaa
    13381 gaacatcatt cagccctttg catagagaaa tggcttcatc aagtagccag gcaccgttgg
    13441 gatcaacggt aatccgcgca tcagggaagc gtttcttcaa tgcacgaaca gtgtcgattt
    13501 cttgctcgcc aggtaacacc ccgcccttaa gtttgaaatc tttaaagcca tagcgatcct
    13561 gtgaggcttc cgccagacgc acaacggctt cgctgttcat cgctttctga tggcgcaact
    13621 gataccactc atggttgccc ggcgtatttt ccagataagg aagatcggtt ttggtccgat
    13681 caccgatata aaacagataa ccgaggacgg taatagtctc gcgttgcttg cctggcccca
    13741 acagttcgca gaccggaaca ttcagcgcct tacctagcag gtcaagcaag gcggcttcca
    13801 gcgccgccac ggcattaacg tgcaattcaa aagtccaggc acctttgccg aaggtatcaa
    13861 aatcggctgc ctgattacct ttatgcacct gctgaaccac tttattcagg cgcgcaactt
    13921 cctggcccag aaccatcgga atagcatcga ccagtgtttg ataaatcacc tctccgcccg
    13981 gcgcttcacc aatgccggta tgcccggcgt tatcggtgag taccacaata ttgcgggtga
    14041 aatatgcgtt atgtgcccca ccaatattaa gcaacatgct atcgtgcccg gcgaccggaa
    14101 tgactttcat atcagtaata acaggactgg attgtgtcgt catcttattg ccccgcgaca
    14161 ggtttcagct cgatacgctt gatatcgccc accagcacca ggtagctcag taccgcgatt
    14221 aaggcatgaa caccaacata aatcagcgcc ccattaaagg agccagtcgt gccaacgata
    14281 taaccaattg cgattggcgt gacgatgcca gaaatgttac cgaacatgtt gaacaggcca
    14341 ccggaaagac cgctgatctc ttttggcgcg gtatctgcca ttactgccca acccagcgcc
    14401 ccgatgccct taccgaagaa ggccagcgcc ataaagccga tgatcatcca ctcaacgttg
    14461 acgtagttgc agaacaccat caccatcgac agcaacatgc ccattacgat cggtgtttta
    14521 cgcgcaatgt tcagcgatcc cgtgcggcgc atcagccagt cggaaataat cccacccagc
    14581 acaccgccga taaaaccgca aaccgccgga acggaagcca caaagcccgc tttcagaatt
    14641 gacatcccac gcgcctgcac cagataaacc ggaaaccagg taatgaaaaa gtaagtcagg
    14701 gcgttgatac agtactgacc gatataaacg ccgatcatca tccgtgaccc gagcagttgt
    14761 ttgatctgcc cccacttcac gctgaacggc actttaactt tggtgttttg ctgatccata
    14821 ttgatcagcg cgccacccgc ggcgatgtac tccagctctt tctgatttac ccccggatgt
    14881 tgatttggct cgtggatgac tttcaaccag ataaagctga tgacaatccc cagaccgccc
    14941 ataaagaaga agacgtgtga ccagcccact tcatgcgtca gccagcccat aatcggcgcg
    15001 aagatcaccg ttgcgaagta ttgagcggag ttaaaaatcg acaccgccgt tcccctttcc
    15061 tgcgccggaa accaggccgc aacaatgcga ctgttgccgg ggaaagatgg cgcttcagca
    15121 agcccgacca ggaagcgcag agtaaacagg gcaacgataa tgccgaatcc actaaagata
    15181 tcgacgaagc cttgcagcaa ggtaaacatc gaccagataa agatcgacca gaagtagacg
    15241 cgttttgaac caaaacgatc cagcaaccaa ccaccaggga tctgcccgat aacataagcc
    15301 catgagaagg cagagaacac atagcccatt cccacgggat caaggccgat atctttggcc
    15361 atttccgaac cggcaataga aagcgtagcg cggtcgccgt agttgaagga tgtgacgata
    15421 aacaacatca ccactatcca gtaacgagca ttcgtgcgtt tttccacact gctcgcagcc
    15481 tgacttaaag aactcgttgt tgcactcctg aaaattcgcg ttagccacgc taactctgga
    15541 ctgcgacata gccaggaagt cagaggtgac gtagggtgtt ttttgccgtt tttataggtc
    15601 gttcgccgaa tacggcgcgt gtttatatct ggcaatagca gtataaaaag cgcgccatag
    15661 cggctcaccg tgcaacaaca caacattaat gcgttcaatg aggcccgatt ttggcattag
    15721 ccctggacgg tggaatccac ttcacggaaa tgaaaacaag aacaagaaag gaagggttaa
    15781 aacgaagaaa taaagaagag tatgaaatgg atcgcttgac tccaggcaaa cgccagtaaa
    15841 aatccgcgct atgaagcagt ttttactggc atttgcctga aaagattcga ttcagcaccg
    15901 ctaaaacgac atttaccgct cgctgaacat atcaggacaa cagcgtgccc cactgttcga
    15961 cccacggatt tgattccgtt tccggttccg ggttctcact ggcgtcaatc aacagcattt
    16021 cgccaactcg ctgagcgctc tgttcctgca acaaggcatc gaactgtttg ccgccattgc
    16081 agaaatttac ataactgctg tcgccgagcg caatcacgcc ataacgcaga ttcggctgga
    16141 agcccagact atctttgatt ccctgaaaga gcggcacaat gctatcagga aggtcgccct
    16201 gcccggtcgt ggacgtaacc accagaacat acttatcctg ataaggcagc cagtcgctta
    16261 attcaggatc ttcaaatacc gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt
    16321 cttccgccac taacagcgaa tttccgtaca tggtgccgac aaaaatacca atttccgcca
    16381 taccgttttc tccctggatt aggaacttat ctctccatcc tgacccgatg gcgcactgaa
    16441 ctcaaccctt tcattttcag gaagcagacc gcgccagcca aattgtgata acgcctgcat
    16501 ccaggtgtcg tccaggcccg cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa
    16561 tgacaactga ctggcatgta gcattaaccg ctggaggcca aaatgctcag caccgctgcg
    16621 attctggcgt aaatcgccat gtttgctatc gccaataatc ggatgacgca aatgggcaag
    16681 atgtcggcga agctgatgtt tgcgtccggt tttcggctcc agttccacca ggccgtagcg
    16741 cgtggtcggg taacgtccgg tcgctaccgg catttctacg gtcgccagac cgcgataatg
    16801 cgtcactgct ggctgcgggc ctttatcttc gcgggcaaat ttatcagcga ttttgtccag
    16861 ttcttccacc agtggataat ccagcaccgc ttcttccatc aaccagccgc gcacaatcgc
    16921 atggtaacgt ttctggattt ggtgctgttc aaactgttgt gccagcagcc gtccggcctc
    16981 gctggataat cccatcaaca acacaccaga agtgggtcgg tccagacgat gagcagtaaa
    17041 aacatgctgt cctatctggt tacgcacggt ttgcatgacc actactttct cgtcgcgatc
    17101 cagccagcta cggtgaacca gccagccgga gggtttattt accgaaacca gccattcatc
    17161 ctgatagagt atttccagca ttagctcgca tcatccgcaa aaagagcatc cagtttttcc
    17221 aactcagcca gaataagcgc gtgttgcgga tggtccgtcg ccagcgccat ttcataatag
    17281 ggtgcaacag caaaagcgcc cggtaacggc tggttgttat ttaacaaatc gtgcattcgc
    17341 gggatcagca cccactgcaa ccactccagt ggttccatgg tgtccataaa gaacggttgg
    17401 gtactattaa attgatgcgg ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt
    17461 aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat gagtggtcat aaagcaacct
    17521 caataagaaa aactgccgcg aaggatagca ggaataaaaa aagggagcac tgtattcaca
    17581 gcgctcccgg ttcgtttcgc agcattccag ctacttttgt tgctccctgc tcatccttga
    17641 caacttttcc tctggccttg cggccaatcg ttcatcctga actattgctt cctgctcaca
    17701 ccaccccgat gtgatacttc atcctgaagt gtccttggcc ttcctgacca gccgaatcat
    17761 cctgaccggt tctcattctc catcctggag gtgtccttta acgcgtcctg cgtcatcctc
    17821 ttcgcttcat ccagaagcct ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc
    17881 atcatcctga tgttcacttc gttgtctgac tccctgtcga cgaagataga atcgtctttt
    17941 tcgcgaagtc ttacaaggcg cttacagaca atgccttaag gaaaatttca tatgaaagtt
    18001 aagaattaac aatcaacatt ttgattttaa atgaaaataa cgattcttat ctttttaagt
    18061 attcgcttat ttcctggcga tctcctacag agtctgtaag agatctctca caaccagatg
    18121 gggtatttgg attacagaag aggcttaagt tgattaagga attccgcaag atttgaggct
    18181 aaatgcgtcc gtttacgtgt tcccagcgtc tctttgcaca cttcaccgga gagattacac
    18241 accgagatga cctctaactc ttcttccagc gtcgcgataa atagcgtggg cggcagtttt
    18301 agccttttct gagtcaccag atgaccaatc agattttcct gcacccgacg gaagtcgtct
    18361 tcactccagg tttgcagcaa tgtcagtttg atatcgccaa actgcgcgtg catatccccg
    18421 gcaaactgag tcgtataaaa ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt
    18481 tcaaccgcgt ttacattttg ttccccggta aacggctggg gttgccagta tacggcatct
    18541 tcggtagttg aaataatgca cggtgaagga acaccgtaca gttcctcact taacggccag
    18601 cttttatgct cttcatgcca cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt
    18661 gcggtcaaat cgtccactaa tttctctctt cacttaaacc agatacactt gtcgtttagt
    18721 ttatctggtt tatgacggtg aaacatgtct tcttatgcaa accatcaggc acttgcgggc
    18781 ctgactcttg gaaaatcaac cgattaccgg gatacctatg acgccagcct actgcaaggc
    18841 gttccacgca gcctgaatcg cgacccgctg ggtctgaaag cggataacct gccttttcac
    18901 ggtacggata tctggacgct gtatgaactt tcctggctga atgcgaaagg tttgccgaag
    18961 gtcgctgtcg gtcatgttga acttgattac accagcgtaa atctgattga gtcgaagagt
    19021 tttaagctct atctcaacag ttttaaccag acgcgtttta ataactggga tgaggtgcgc
    19081 cagacgctgg agcgcgactt aagcacttgc gctcagggta agattagcgt ggcgttatat
    19141 cgtcttgatg aactggaagg ccagccgata ggtcatttta atggcacttg cattgatgac
    19201 caggatatca ctatcgataa ctatgaattc actactgact atctggagaa tgccacctgt
    19261 ggtgaaaaag tagtggaaga gacgcttgtc agccacctgc tgaaatcaaa ctgcctgatc
    19321 acccatcaac cagattgggg ttcgatccaa attcagtatc gtggacgcca aattgacaga
    19381 gaaaaactgc tgcgttacct ggtctcattc cgtcatcaca acgagttcca cgaacagtgc
    19441 gtggaacgca tctttaatga cctgttacgc ttctgccagc cagaaaaatt gagcgtttac
    19501 gcacgttata cccgtcgtgg cggtctggac attaacccgt ggcgcagtaa tagcgatttt
    19561 gtcccatcga ccacaagact ggttcggcaa taaatttttt ctcaattttg cgtgctggat
    19621 tcacgcagaa ggttgtgaaa ggtcatcagg cagggctatt gtaatcaaag ggaatgacga
    19681 tattcgtccc ataaggagtt tttcttgatt acacatatta gcccgcttgg ctccatggat
    19741 atgttgtcgc agctggaagt ggatatgctt aaacgcaccg ccagcagcga cctctatcaa
    19801 ctgtttcgca actgttcact tgccgtactg aactccggta gtttgaccga taacagcaaa
    19861 gaattgctgt ctcgttttga aaatttcgat attaacgtct tgcgccgtga acgcggcgta
    19921 aagctggaac tgattaatcc cccggaagag gcttttgtcg atgggcgaat tattcgcgct
    19981 ttgcaggcca acttgttcgc ggtcctgcgt gacattctct tcgtttacgg gcaaatccat
    20041 aacaccgttc gttttcccaa cctgaatctc gacaactccg tccacatcac taacctggtc
    20101 ttttccatct tgcgtaacgc tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc
    20161 tgctggggcg gtcactcaat taacgaaaac gagtatttgt atgcccgtcg cgtcggaaac
    20221 cagctgggcc tgcgtgagct gaatatctgc accggctgtg gtccgggagc gatggaagcg
    20281 ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt acaaagacag tcgttttatt
    20341 ggtatgacag agccgtcgat tatcgccgct gaaccgccta acccgctggt caacgaattg
    20401 atcatcatgc cagatatcga aaaacgtctg gaagcgtttg tccgtatcgc tcacggtatc
    20461 attatcttcc ctggcggtgt gggtacggca gaagagttgc tgtatttgct gggaatttta
    20521 atgaacccgg ccaacaaaga tcaggtttta ccattgatcc tcaccggccc gaaagagagc
    20581 gccgactact tccgcgtact ggacgagttt gtcgtgcata cgctgggtga aaacgcgcgc
    20641 cgccattacc gcatcatcat tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg
    20701 atgccgctgg tgaaagaaaa tcgccgcgac acaggcgatg cctacagctt taactggtca
    20761 atgcgcattg cgccagattt gcaaatgccg tttgagccgt ctcacgagaa tatggctaat
    20821 ctgaagcttt acccggatca acccgttgaa gtgctagctg ccgacctgcg ccgtgcgttc
    20881 tccggtattg tggcgggtaa cgtaaaagca gtcggtattc gcgccattga agagtttggt
    20941 ccttacaaaa tcaacggcga taaagagatt atgcgtcgta tggacgacct gctacagggt
    21001 tttgttgccc agcatcgtat gaagttgcca ggctcagcct acatcccttg ctacgaaatc
    21061 tgcacgtaat ctcccctccc ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac
    21121 ttactcatca actcatttca tttgttatat gaatgtttct taccaccctc acgcgacaaa
    21181 tatcatcaca gttaatatgt catacaattt atgttgcaac gcaaacgttt ctctattttc
    21241 ataaaccgta ttttatcgct atgaaaaaga aattatcgcc atgattaact aaaagtattg
    21301 attttttcag ttcaacctac atatattgcg cgccctggaa gaagtcagat gtcgtttaat
    21361 gggcaaatat tgcccttaaa ttctctttta cttttgattt acagagtaaa gcgttgggat
    21421 aatctatctt ccaagtagat tattgtattt gagatcaaga tcactgatag atacataact
    21481 tgtgtgtatc tttccgccct caaattatta cggcggtaaa tgattaagcc atcgccgata
    21541 gacagatttc atttttacgg tcaggcacct tcccgggctg aactggctaa aagctgaatt
    21601 atttgcattc ctccaggaga aatagatgga aacgactcaa accagcacga ttgcgtcgaa
    21661 agactctcgt agtgcctggc gcaagacaga caccatgtgg atgctgggcc tttacggcac
    21721 ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc
    21781 gctgatcatc atggctatcc ttgcgttccc gatgacgttt tttgctcacc gcggcctgac
    21841 tcgcttcgta ctgtctggta aaaacccggg cgaagacatc accgaggttg tagaagaaca
    21901 ctttggtatt ggcgcaggta aactgattac cctgctctac ttcttcgcta tctacccgat
    21961 cctgctggtt tatagcgtgg caatcaccaa tacagttgaa agcttcatgt ctcaccagct
    22021 gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac
    22081 catcgttcgc ttcggtgagc agatgatcgt taaagcgatg agtattctgg tgttcccgtt
    22141 tgttggcgta ttgatgctgc tggctctgta cctgatcccg cagtggaacg gcgcagcact
    22201 ggaaacgctg tctctggaca ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg
    22261 gctggcaatt ccggtaatgg tgttctcgtt caaccactct ccgatcatct cttctttcgc
    22321 cgttgcgaag cgtgaagagt acggcgatat ggcagaacag aaatgctcga agatcctggc
    22381 attcgcacac atcatgatgg tgctgaccgt aatgttcttc gtcttcagct gtgtactgag
    22441 cctgactccg gcagacctgg ctgcggctaa agagcagaac atctcgattc tgtcttacct
    22501 ggctaaccac tttaacgcac cggttatcgc gtggatggct ccgattatcg cgattatcgc
    22561 tatcaccaaa tccttcctcg gccactacct gggcgcacgt gaaggcttca acggtatggt
    22621 gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc aacaagctga accgtatcac
    22681 tgcgctgttc atgctggtaa cgacctggat tgttgccacc ctgaacccga gcatcctggg
    22741 tatgattgaa accctgggcg gtccaatcat cgcgatgatc ctgttcctga tgccgatgta
    22801 cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt cacatcagca acgtattcgt
    22861 tgtcgtgatg ggtctgattg caatctccgc aatcttctac tctctgttca gctaagtcct
    22921 ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc gatgtatttc ctatgattag
    22981 cgtattcgat attttcaaaa tcggcattgg cccttccagt tctcataccg ttggaccaat
    23041 gaaagcgggt aaacaattta ccgacgatct gattgcccgt aacctgctta aagacgtgac
    23101 ccgcgtggtg gttgacgtgt acggctcgct ctctctgacc ggtaaaggcc accacactga
    23161 tatcgccatt attatgggcc tggcgggtaa cctgccggat accgtggata tcgattccat
    23221 ccctggtttt attcaggatg tgaatactca tggtcgcctg atgctggcaa acggtcagca
    23281 tgaagtggag ttcccggttg atcagtgcat gaacttccac gccgacaacc tttctctgca
    23341 tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa gtcgtttaca gccagactta
    23401 ctactctatt ggcggtggct ttatcgttga tgaagagcat tttggccagc aggatagcgc
    23461 accggttgaa gttccttatc cgtacagttc agcagccgat ctgcaaaaac attgtcagga
    23521 aaccgggctg tcactctctg gcctgatgat gaaaaacgag ctagcgctgc acagcaaaga
    23581 agagctggaa cagcacctgg cgaacgtatg ggaagtcatg cgtggcggta ttgagcgcgg
    23641 tatttccacc gaaggcgtgt tgcctggcaa actgcgcgtt ccacgccgtg ctgcggcact
    23701 acgccggatg ctggtcagcc aggataaaac caccactgac ccgatggcgg ttgttgactg
    23761 gatcaacatg tttgcactgg cagtgaacga agagaacgct gctggcggac gcgtggtgac
    23821 tgcgccgact aacggtgcgt gcgggattat cccggcagtg ctggcgtact acgacaagtt
    23881 tatccgcgaa gtgaacgcta actcactggc tcgttacctg ctggtagcca gtgccattgg
    23941 ttctctttat aagatgaacg cgtcaatttc tggtgctgaa gtcggctgcc agggtgaagt
    24001 tggtgtggcg tgctcaatgg cggcggctgg tctggcagaa ctgttaggtg caagcccggc
    24061 gcaggtgtgc atcgcggcgg aaatcgccat ggagcacaac ctcggtctga cgtgtgaccc
    24121 ggtcgccgga caggtccagg tgccatgcat cgagcgtaac gccattgcgg cagtaaaagc
    24181 ggtgaacgcc gcacgtatgg cgctacgccg taccagcgag ccgcgcgtct gcctcgataa
    24241 agttatcgaa accatgtacg aaacaggtaa agatatgaac gccaagtacc gcgaaacctc
    24301 tcgcggcggc ctggcaatga agatcgttgc ctgcgattaa tcgctctcca gaggcctcgt
    24361 tttgcgaggc ctcttcccga tttctcatcc agccgtagcc tgttccggca tcgaatgtta
    24421 cccttatcgc ctgatcttta agggggttat cgtggctgtt catttgctta ttgtcgatgc
    24481 actgaatctt attcgtcgca ttcatgccgt tcagggatcg ccctgtgtcg aaacctgcca
    24541 gcatgcgctc gatcagctca ttatgcacag ccagccaacc cacgcggtcg ccgtttttga
    24601 tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta ccagattaca aagcgggtcg
    24661 accgccaatg ccggaagagt tgcacgacga gatgcctgca ttacgcgccg cctttgagca
    24721 acgcggcgtc ccgtgctggt caaccagcgg caacgaagcc gatgacttag ccgccacgct
    24781 ggcggtcaaa gtgacacagg ccgggcatca ggcaacgatt gtttcgacag ataaaggcta
    24841 ctgtcagtta ctttcaccga cattacgtat tcgtgattac ttccagaaac gttggctgga
    24901 tgcaccattt atcgataaag aatttggcgt tcaaccgcag cagttgcccg attactgggg
    24961 acttgcgggg atcagcagtt caaaggtacc gggtgttgcg ggaatcggac caaaaagcgc
    25021 cacgcagctg ctggtcgagt ttcagagtct ggaagggata tatgagaatc tggatgcggt
    25081 tgccgaaaag tggcgcaaaa aattagaaac ccataaagag atggcgtttc tgtgccgcga
    25141 tattgcccgc ttacaaaccg atttgcatat cgacggcaat ttacagcaat tgcggttggt
    25201 acggtaacgg cgagccggat gcaaacgatt atcaacacat tgaatttaat gcacttatat
    25261 accggataag acgcggctag cgtcgtatcc ggcattatca catcagcgca tttaccaggc
    25321 ggtatggtaa agctctacaa tatcctcaag cgttgcttca cgcgggttgc caccggtaca
    25381 aacatcatcc agtgccgcct gcgccagtgc cggaatgtct tccttgcgta caccaacatc
    25441 acgcaaatgt ggcggaatac cgacatcacg gttgagagca aacaccgctt caacagcggc
    25501 attacgcgcc tcttccagcc actttcacgc ccataacgcg cgcgatatcg cggtacttct
    25561 caccggtaaa gtcagcgtta tagcgcatga catgcggtaa caggatggcg ttcgcaacac
    25621 cgtgtggagt gttgtaaaac gcgcccagtg gatgcgccat accatgcacc aaccctaacc
    25681 caacattcga gaagcccata cccgcaacat actgcccgag cgccatttct tctccggcat
    25741 ccttatcacc agcaaccgat cctcgcagcg ccccagcaat gatttcaatc gctttaatgt
    25801 gcagtgcatc ggttagcgcc cacgcgccac gggtaatata cccctcaata gcatgagtga
    25861 gcgcatcgac acccgtcgca gctttcagcg ctggaggcat accatccatc atgtcagcgt
    25921 caataaacgc cacctgcggg atatcatgcg gatcaacgca aacaaacttg cgccgtttct
    25981 cttcgtcagt gatcacgtag ttaatggtca cttctgccgc agtacctgct gtggtaggaa
    26041 ttgccagaat cggtacactg ggtttattgg tcggggaaag cccttccagg ctacgcacat
    26101 cggcaaactc cgggttgttg ctgataatgc caatcgcttt acaagtatcc tgtggagaac
    26161 caccaccaat agcgatcagg taatccgcgc cgctattctg gaatacaccg agcccttctt
    26221 tgacgacagt aattgttggg ttgggcacta cgccgtcgta aatcgcccat gccagccctg
    26281 cagcatccat cttatcggtc actttcgcca ccacgccgca ttgcaccagc gttttatcgg
    26341 tgacgatcag cgccttctga taaccacggc gtttcacctc atcggttaaa gccccaacag
    26401 caccccgacc aaaccatgcc gtttcgttca gaatcattct gttagccatc atccttctcc
    26461 ttgttgcttt acgaaattac tcttcaatcc gtaacccata ggttttgaat ttctccagca
    26521 ctacggcaat ctcttcatcg ctcagcactg gcaccgggtc cgtaatcgcc agggtcgtca
    26581 ggtaaagttg cgccagcact tcaacttcat gcgccagcca taacgctttt tccagattca
    26641 cctcacaagc gataagccca tgatgttgta acaaagttgc cttacgattt ttgagagcca
    26701 gcgcaacatg ttcagaaagt tcgcgtgttc caaaggtcgc ataaggcgcg caaggaatag
    26761 aattaccacc tgccgccgca atcatgtagt gaatagcggg gatcggtcgg ttaagaatgg
    26821 aaactgctgt gcaatgaacg gcatgattgt gaacaaccgc gttggcatcc ggtctgcttt
    26881 gataggccgc catatggaaa cgccattcgc ttgaggggag ctttccttcc tcatgtttac
    26941 cgttaccatc aataaaggca atatgcgact ccgtcagttt ttcatatgga atgcctgtag
    27001 gcgtaatcag catcccatcc tgataacgta cactgacgtt ccccgctgtc ccctggttca
    27061 gtcccaggcg ggtcatttcc aggcaagtgt caataatctg acgagcaagt ttatttcgtt
    27121 ccattagcta cctctctctg attcaaaaca gggcaataat gttgttcctt tcacactatt
    27181 gaattagccg tttaattacc caccat
//
LOCUS       DAJTNN010000038        27170 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTNN010000038 DAJTNN010000000
VERSION     DAJTNN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.038"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..517
                     /locus_tag="OO150_001429"
     CDS             <1..517
                     /locus_tag="OO150_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5515623.1"
                     /translation="IMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAEIINK
                     KHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNG
                     GRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLM
                     PLIRRRTMLQL"
     gene            883..1950
                     /locus_tag="OO150_001430"
     CDS             883..1950
                     /locus_tag="OO150_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5515624.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1947..4682
                     /gene="rbbA"
                     /locus_tag="OO150_001431"
     CDS             1947..4682
                     /gene="rbbA"
                     /locus_tag="OO150_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5515625.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDMWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4682..5806
                     /locus_tag="OO150_001432"
     CDS             4682..5806
                     /locus_tag="OO150_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5515626.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5879..6153
                     /locus_tag="OO150_001433"
                     /pseudo
     CDS             5879..6153
                     /locus_tag="OO150_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6150..6509
                     /locus_tag="OO150_001434"
     CDS             6150..6509
                     /locus_tag="OO150_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5515627.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6629..7030)
                     /gene="nikR"
                     /locus_tag="OO150_001435"
     CDS             complement(6629..7030)
                     /gene="nikR"
                     /locus_tag="OO150_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5515628.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7036..7842)
                     /gene="nikE"
                     /locus_tag="OO150_001436"
     CDS             complement(7036..7842)
                     /gene="nikE"
                     /locus_tag="OO150_001436"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5515629.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7839..8603)
                     /gene="nikD"
                     /locus_tag="OO150_001437"
     CDS             complement(7839..8603)
                     /gene="nikD"
                     /locus_tag="OO150_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5515630.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8603..9436)
                     /gene="nikC"
                     /locus_tag="OO150_001438"
     CDS             complement(8603..9436)
                     /gene="nikC"
                     /locus_tag="OO150_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5515631.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSH
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9433..10377)
                     /gene="nikB"
                     /locus_tag="OO150_001439"
     CDS             complement(9433..10377)
                     /gene="nikB"
                     /locus_tag="OO150_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5515632.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQFFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10377..11951)
                     /gene="nikA"
                     /locus_tag="OO150_001440"
     CDS             complement(10377..11951)
                     /gene="nikA"
                     /locus_tag="OO150_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5515633.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12062..12649)
                     /gene="acpT"
                     /locus_tag="OO150_001441"
     CDS             complement(12062..12649)
                     /gene="acpT"
                     /locus_tag="OO150_001441"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5515634.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12704..13753)
                     /locus_tag="OO150_001442"
     CDS             complement(12704..13753)
                     /locus_tag="OO150_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5515635.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVTITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMIGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYACILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13885..15102
                     /locus_tag="OO150_001443"
     CDS             13885..15102
                     /locus_tag="OO150_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5515636.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15106..15663)
                     /gene="dcrB"
                     /locus_tag="OO150_001444"
     CDS             complement(15106..15663)
                     /gene="dcrB"
                     /locus_tag="OO150_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5515637.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAENNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15736..16401)
                     /locus_tag="OO150_001445"
     CDS             complement(15736..16401)
                     /locus_tag="OO150_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5515638.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16622..16867
                     /gene="tusA"
                     /locus_tag="OO150_001446"
     CDS             16622..16867
                     /gene="tusA"
                     /locus_tag="OO150_001446"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5515639.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16969..19167)
                     /gene="zntA"
                     /locus_tag="OO150_001447"
     CDS             complement(16969..19167)
                     /gene="zntA"
                     /locus_tag="OO150_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5515640.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEYKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19241..19867)
                     /locus_tag="OO150_001448"
     CDS             complement(19241..19867)
                     /locus_tag="OO150_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5515641.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYF"
     gene            20008..20367
                     /locus_tag="OO150_001449"
     CDS             20008..20367
                     /locus_tag="OO150_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5515642.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20370..20639)
                     /locus_tag="OO150_001450"
     CDS             complement(20370..20639)
                     /locus_tag="OO150_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5515643.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20629..21225)
                     /gene="rsmD"
                     /locus_tag="OO150_001451"
     CDS             complement(20629..21225)
                     /gene="rsmD"
                     /locus_tag="OO150_001451"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5515644.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21375..22868
                     /gene="ftsY"
                     /locus_tag="OO150_001452"
     CDS             21375..22868
                     /gene="ftsY"
                     /locus_tag="OO150_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5515645.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22871..23539
                     /gene="ftsE"
                     /locus_tag="OO150_001453"
     CDS             22871..23539
                     /gene="ftsE"
                     /locus_tag="OO150_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5515646.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23532..24590
                     /gene="ftsX"
                     /locus_tag="OO150_001454"
     CDS             23532..24590
                     /gene="ftsX"
                     /locus_tag="OO150_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5515647.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24835..25689
                     /gene="rpoH"
                     /locus_tag="OO150_001455"
     CDS             24835..25689
                     /gene="rpoH"
                     /locus_tag="OO150_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5515648.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25960..27063
                     /gene="livJ"
                     /locus_tag="OO150_001456"
     CDS             25960..27063
                     /gene="livJ"
                     /locus_tag="OO150_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5515649.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGALKGFEFGVFDWHANGTATDAK"
BASE COUNT         6409 a         7051 c         7595 g         6115 t
ORIGIN      
        1 aataatggca aatcatcatg ggataatttt tgatgagcag cgaaaacgtt acattatagg
       61 atcgataaca gtctctattg atgaaatgac caatgctctc gatccggtcg aattagcgga
      121 aattattaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa atagccgggc
      181 tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca gacctaaact
      241 tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg cacataacgg
      301 taatggtgga agagaatgta taggtgaaac ggggacagtt ttaggtctta acgcccgtga
      361 cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc ctggtgcagg
      421 gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa atggacttat
      481 gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca tgaatattaa
      541 tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg ttcctgcaac
      601 tgaacaggcg tgaaacgcga tccaacagga tggataccgt tttgtcagcc agttttatag
      661 atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga tgacaacatt
      721 actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg ctggtcgtat
      781 ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg gcggcagttg
      841 taacacgcgt cgtttgctta cagggattat ggcgggccga ttatggataa gagtaagcgc
      901 catctggcgt ggtgggttgt cggggcactg gcggtggctg ccgtcgtggc gtggtggctg
      961 ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat tgaagcgacg
     1021 gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt gaaagaaggc
     1081 cagtttgttc gcgaaggtga agtgctggcg aagatggata ctcgtgtgtt gcaggaacag
     1141 cgactggaag ccatcgcgca aatcaaagag gcacaaagcg ccgttgccgc cgcgcaggct
     1201 ttgctggagc aacgacaaag cgaaactcgc gccgcgcagt cgctggttaa tcaacgccag
     1261 gcagaactgg actccgtagc aaaacgtcat atgcgttccc gttcactggc ccaacgaggg
     1321 gctatttctg cgcaacagct ggatgacgac cgcgccgccg ctgagagcgc ccgagctgcg
     1381 ctggaatcgg caaaagctca ggtttctgct tctaaagccg ctatagaggc ggcacgtacc
     1441 aatatcattc aggcgcaaac ccgcgtcgaa gcggcacaag ccactgaacg gcgcattgcc
     1501 gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca gtatcgggtt
     1561 gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt cgatctcagc
     1621 gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctaaa actgggcggt
     1681 gaagcacggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac catcagtttt
     1741 gtcgccagcg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga acggctgaaa
     1801 ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca tctggaatat
     1861 gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga atgaagaact tccgtggcct
     1921 gacgacctgg tggtgaggtt gccgcaatga cgcatctgga actggttccc gtcccgcctg
     1981 tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg aacaatatca
     2041 ctctcgatat tccggcccgc tgtatggtcg ggctgattgg cccggacggc gtcgggaagt
     2101 cgagtttgtt gtcgttgatt tccggtgctc gtgtcattga gcagggcaat gtgatggtgc
     2161 tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc atcgcctgga
     2221 tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa aacgtcgatt
     2281 ttttcgcccg cctgttcggt cacgacaaag cggagcggga agtgcgaatt aatgagctgc
     2341 tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc tccggcggga
     2401 tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg ttgatccttg
     2461 atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat ctgatcgaca
     2521 gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat atggaagagg
     2581 ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca accggcagcg
     2641 ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt attaatctgt
     2701 taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat caacctgaaa
     2761 acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc ttcgttgccg
     2821 ttgatcacgt caatttccgc attccacgcg gggagatttt tggattcctt ggttcgaacg
     2881 gctgcggtaa atccaccacc atgaaaatgc ttaccggact gctgcccgcc agcgaaggtg
     2941 aggcgtggct atttgggcaa ccggttgatc caaaagatat cgatacccgc cgtcgtgtgg
     3001 gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa aacctggagt
     3061 tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg gctgaaatga
     3121 gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg ccgctcggca
     3181 ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg ttaatcctcg
     3241 atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag ttgatggtcg
     3301 atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg aacgaagcgg
     3361 aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc ggtacaccgc
     3421 aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc gcctatttgc
     3481 aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac gacaccaccc
     3541 acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc cgcgaagcgc
     3601 tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg gtgatcctga
     3661 tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt gcggtgctcg
     3721 accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt tcccgttact
     3781 ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg cgtgcgggcg
     3841 atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg cgtggtacgc
     3901 ctgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa acggtaaaag
     3961 gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga caatcgacac
     4021 ccgccagcca aagcgggctg atgaacattg agacgcgtta tcgctataac ccggacgtaa
     4081 aaagcctgcc agcaattgtt ccggcggtga tcccgcttct gctgatgatg atcccgtcaa
     4141 tgctaagcac ccttagcgtg gtgcgggaaa aagagcttgg gtcgattatc aacctttacg
     4201 tgacccccac cacgcgtagt gaatttttgc ttggtaaaca gttgccatac atcgcgctgg
     4261 ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc gtaccgcata
     4321 aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt gccaccggaa
     4381 tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc ggaacggcga
     4441 ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta gcttcgctgg
     4501 aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg actatcgccc
     4561 gcgggacgtt ctcgaaagcg ctggatctga ctgatatgtg gcaacttttt atcccgttgc
     4621 tgatagccat cccgctggtg atgggcttaa gtatcctgct gctgaaaaaa caggagggat
     4681 gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca gtctgctcgg
     4741 tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt attcgtcagc
     4801 gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata tggatcaatc
     4861 gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc caccggagat
     4921 gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct tcgcgataaa
     4981 tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata ttcaggtgaa
     5041 cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc agaatattat
     5101 caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac cgttggtatc
     5161 gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg gcggggtgat
     5221 ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg cgctgatccg
     5281 cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc cgtttgagat
     5341 catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg gattatcgct
     5401 ggtgctgatg gtgaaaggtg tactgggcgt accgattgaa ggctcgatcc cgctgtttat
     5461 gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta tggggacgat
     5521 agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc cgctgcaaat
     5581 gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg acattatgct
     5641 gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc ggggtgccgg
     5701 attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg cgtttttcac
     5761 cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa gggaaacttg
     5821 acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag gacatgccat
     5881 gagaaccaag gtacaggctt tacggaagaa acaaaaaata cattggatca aatatttaaa
     5941 actcctgttc ctcaagggat caagtggtct gatatagagt cactggttaa agcattaggc
     6001 ggagaaatta aggaaggaag aggttcgcgt tgtaagttca tactaaatat gagcgttgcg
     6061 tgtttccatc ggcctcatcc gtcgccagat accgataaag gcgctgtaga aagcgtgcgt
     6121 gactggttac taagtatagg ggtaaaacca tgatcaaact gaagacgcca aactctatgg
     6181 agattgccgg gcaaccagct gttattacct acgtgccaga actgaatgca tttcgtggca
     6241 aattcctggg cttgtcaggt tattgtgatt ttgtctctga cagcattcag gggctgcaaa
     6301 aagaagggga actttccctg ctagagtatc ttgaggattg taaagctgcg ggtattgagc
     6361 cgtatgcgcg tacagaaaag attaagacct ttacgttacg ctatccagaa tcgctgagtg
     6421 aacgccttaa taatgcggct gcgcagcaac aagtctctgt taatacctat attattgaaa
     6481 cactgaatga gcgtttgaat catttataaa ttttagcggc gtacattacg cagaatatct
     6541 gcctttacag gatctatcta tgcaactgaa cttaatttta taaattaagt tcagttaata
     6601 tcttttcttt cctcgtttca gaaataactc aatcttcctt cggcaagcac tgcaaatgcc
     6661 catgccgcac gccgcgctgg gcgataacgt catcggcaaa atgctgcacg tcgcccatat
     6721 cacctttcag cacggcgatt tccagacagt cgtcgtgatt gatatgcaca tgcagcgtgg
     6781 cgacggagag atcgtggtga tggtgctggg tggagacaat gcggctggct aagtcgcgtt
     6841 tttcgtgttc atacacatag gagagcaccg cgaaaccttg cgtgccgtgc tgctgagtgg
     6901 cctcctgggc cagggcgcta cgcagaatat cgcggatagc ttcggaacgg ttgttataac
     6961 cacgacgctg gctcaggctg tccagcgtct ccagtaaatc gtcatcaagc gtgatggtga
     7021 ctcgttgcat ttgcgttaaa ccttttctgt ggcgcgacgg cgcacgggga atgcgggtaa
     7081 taccgcgttt tgtagcacac gtccggcgtc agaggaaaag gttaatttat ctcccaccgc
     7141 ctgggtttcg acgatttgtc cgttgtccat caccatcacc cgctggcaaa agcgttctac
     7201 aagacgtaag tcgtgggtga tgaacaggca ggcggtgcca aactgttgtt gcagcttttt
     7261 cagcaggcga atgacgcccg cctgtaacac gagatcaagg ttagaaacgg tttcatccag
     7321 aatcagcaat ttcggttcga ccgccagcgc gcgtgccagg cagacgcgct ggagctggcc
     7381 gccgcttaac tgcggtgggc gtttgtcgag aacgctgtca tcgagatcga ccgccttcag
     7441 catttcgctg gcgcgcgcca gttgttcgga ttttttcagt gatagcaggt ggcgcatcgg
     7501 ttcacgcagg atctcgcgca cggttttgcg cggatttacg gcactgatgg agtcctgaaa
     7561 caccatctga atatcacggc ggaaggcttt acgttgggcg cgattaagtt tcgccagcga
     7621 ttcgccgcgc cagctaatat tcccttgtga gggagactct aagcctacca gcagccgcgc
     7681 gagggtgctt tttccgcagc cgctgcgtcc cagcagggcg acggtttcgc cgcttttcag
     7741 ggtcagggaa acgttattca gcaccgcctg atgttgatgt tttccgctaa atccaccgtg
     7801 ggcatagtga tgggaaaggc cgctgacgtt aagtaaggtc atgatgccag ctccataccg
     7861 tagagggcga gatgagcgga aaccaggctg cgcgtcaccg tatgtttggg ggcgttaaac
     7921 agcgtttcta catcgccctg ttcgacaatt ttaccttgtg acatcaccgc cacgtcatcc
     7981 gccagacgcg ccacaacgcc catatcatgg gtgaccagca gcattcccgg cgcttgtttt
     8041 tgcataatgc tttccagcag atcgaggatg cgcgcctgtg ctaccacgtc gaggtcggtg
     8101 gtcggttcat cggcgatgat aaacggtgat tcacacagca ccgccatcgc aatcatcatg
     8161 cgctgcaaca tgccgccgct catctcgaac gggtacagct tcagcacgcg cgcggcgttt
     8221 tccagcccca ccgcttctat ggcagcggta agcgtagcgt catcggcggg tttccctaac
     8281 gccaggcagg tttcccgcgc gtgggtgtgc atggtgtgta gcggattaaa ggcgctgcgc
     8341 gggttctgca tgatggtggc aattttgatg ccgcgcaggg cgcagggata aaccgctttg
     8401 ccatcggcta aaatttcccc cgccgtctgg cgaacgccag cgggcaaaat gcccagcgtc
     8461 gcggcgcagg ttaatgattt cccgctgccg ctaccgccga ctaacgccag cacgcgcccg
     8521 cgttgcaggg ttaacgatac accgtgtacc agcggctgcg cggcctgtag cgcgatatta
     8581 cgtagttcaa tctgttgcgg cattagtgtg cgtgctccgt caccagatga gggtccagat
     8641 gatcgcgcag tgcgtcaccc accaggttaa aggccatcac gctgataaac agcgccagcc
     8701 ccggccagaa catttgcagc ggctgggtcc agatatactg gcgcgcgtcg ttaatcatca
     8761 cgccccattc ggcggtcggc gcggtcacac cgaggccaag gaaagacatc cccgagacgt
     8821 gcagcatcat atggccgata tccagcgttg ccagcaccag cagcgaaggg atcaccgcgc
     8881 ctgccagatg atcgacaaac actcgcacat ggcccgcacc ggaaagccgt gacgccagca
     8941 caaactcgcg ttggcgtagt gaaatcacca ggctgcgcac catgcgtgca taccacgccc
     9001 agtgcgacag ggcgatggcg ataattacgt tggtcagccc ggtgccgagc acgccaacca
     9061 taaagaacga cagaatcgag gtcgggaagg tcataaacat atcggcgacg cgcatggtgg
     9121 cctgatcaac gcgcccgcca atcaacccgg cgctgccgcc aataaccagc cctaatgtca
     9181 gcaccagcag caggcaggcc attaccgaac cgagcgacac gcgggtcgct gccatcagcc
     9241 gcgagaaaat atcgcgacct aagtgatcgg tgcccagcca gtgctgcgca tccggcgaaa
     9301 gcaggtgcga cggcaaatca atcgcctggg ggtcatacgg cagccaccac tggctggtga
     9361 gcgcaatcag cgccagcagg gcgataatga tcagcgccag gcgtaccgac cagcgggaag
     9421 agaggaaaaa gttcacgcgt gcgctccttc atgacgacga atgcgcgggt ccagcgcggc
     9481 gttgagcaaa tcgacaatca aattacagac cacaaaaacc accaccatca tcagcgtaaa
     9541 gaactgaatc accggatagt cacggttaaa aatcgccgac accgcatagc gcccgacgcc
     9601 aggccaggca aagatgtttt cgataatcat cgtcccgcca atcaattcgc caatgtgcat
     9661 ccccacggcg gtgatcatcg gcagcgaggc attgcgcaga atatggcggc gttcggtctg
     9721 tttgtcattc aggccgcgta gccgcgccca ggtgacgtga cgctgaccgg cgacgtccag
     9781 catactggcg cgcagcagtc gcgcgttaat cgccagcgac ataaaggcga tggaaaccgc
     9841 aggcaaaatg atgtgctgcc agccgccgta gcccatcgcg ggtagccatt gcagatacac
     9901 cgaaaacgcc atcactagca aaaatgccag ccagaagttg ggcatcgaca cgccaagaaa
     9961 cgcgataaag cgcacggcga aatccggcaa acgatcgcgg tggcgcgccg cccagatacc
    10021 gagcggtacg gaagtgagca gaattaatac cagcgccgca cctgcaagtt ccagcgtggc
    10081 gggcaggaag ttcagcatat cgtccagcac cgggcgttgg ctggcgaatg agataccaaa
    10141 gtcaagatgc agcgccttcc acaaccaggt gccgtactgg acgtacagcg gctgatccag
    10201 tccgagcatg gtgcgggtag aggccagcat ctccggcgtc ggcggcaggt tagacagacg
    10261 caaataatcg agcgccgggt cgccggtacc gaggcgcagc atcagaaaaa tgatcaccga
    10321 ggcggcgagc accatcggga tcagcagcag aaagcggcgt aatacgtaac gcaacattaa
    10381 ggtttcaccg gtttaatctg ttcgaacgga atttcggtgg cgatcggcgc gtaggggatg
    10441 ttacccagct ccggttttga taccaccatc attgagatgt aactgatagg cagataaacc
    10501 gcctcgtcat gcagacgggt cagaatgtcg cgatacagcg cctgacgttg cgtttcgtca
    10561 tgggtcgcca gcacttcgcc gatctcttta tcaatcagcg gtttgtcggc taatccttgc
    10621 tgtgcctgga agtcagcgtg cgacggtacg cgcattgaac tcaggaaggc gtgtggatcg
    10681 tatggcgcgc cccaggtgcg gtggaaaatc atgccaaaac gaccgtcgcg ctgacgagca
    10741 tagatactgc tctcttcttc gccaatcagc gagacatctg cgccaatctg gcgcatatca
    10801 gcctgaatga tttccgccat cgatttgctt aacgcatcgg tgccgatgaa cgacagttca
    10861 atgcgcagcg gctgaccatt tttctcgcgg atgtctttgc ccgcaggcag cgtccaaccg
    10921 gctttttcca gcaacgcttt cgctttctgc ggatcgtact ggcgcggttt caggccgagg
    10981 ttggcgtagg gcacagaagg ggcaaacagg gtgtcggcga cctgctgggt gccatacaac
    11041 gcgttatcaa tcagcgattt tttgtttacc gcgtaattaa gagcttcacg tactgccagc
    11101 tcattggtgg gggttttggc ggtattaagc gccagcatca cggtttcgat cggctgtgac
    11161 agttgggtat ggtaagccgg gttctggcta aagcgggcga aggtatcgag cggtaataac
    11221 ccttcgtttc cgtacagcag gtcgatatcg ccagtttcaa acgccaccgc gcgggtagtc
    11281 gggtccggga tgacgttaaa ggtgatcttt ttaatcgctg gcttttcgcc ccagtagttt
    11341 tcgttacgga cgaagacatc gtactgattc agtttcgatt cctgcaaaat ccacggtccg
    11401 gtgccaatcg gcgctttaat tccgttcatg gtttcatggt ttttaaactg cgagggagcg
    11461 ataaaacgga aaggacgggg cagggccagt tcttgcagga aaggatagta ggcgcttttc
    11521 agggtaattt gcagctctgt tttactgagt gctttaacat caacaatctg gtttgccagc
    11581 tccagccagg cgtgacgttg acggttatcg agcactgcgc ggaagttttc tgccgccgcc
    11641 tcggcatcga acggttcacc gttggataat ttcacgtcat cacgcagggt gaaggtccag
    11701 gttttaccat cttctgaatg agtccagctt tttgccagcc acgggatcac cgaaccgtct
    11761 gcctgatatt tcaccaatgg ttcataaacc atgctctggg cgaacatctg gttaggcgtg
    11821 taaaggtgcg ggtttagtgg cccgacattc accggccagg cggtagtgat ttcatctggt
    11881 gcagcggcat ggacgataaa agacgcacaa gccagcagcg caaatagagt gcggcggagt
    11941 gtggagagca tgataacccc aatggattaa aatagatggc ggaaataagt atgacgattt
    12001 taagtattcg tcatactgat tacctgttct ggatcaatag atgggcagtc gggtgggcgg
    12061 atcagttaac tgaatcgatc cattgcacac tgtcggcggt gagcgtaaag ggggtggggg
    12121 tgcagatcgc aaggcttaaa ttttctagct gacaatggct gactgacagc gaggagtgat
    12181 aggtgctatc tacgctgacg atttgccagg cgctgccgcc acgctgttta acgatggctt
    12241 ctttgcgcgt ccagatgcgc cagaacattt ccagttgctg atcaggatgc acggcgtcca
    12301 tttcagcgtg ttccccgagg ctgaacacgg cgttcgccag ccagcgccag ttggcgcgcg
    12361 ggcgaatcac ttcgatatcg cagccgactt cgccttcatc gctcaacagc agagcgatat
    12421 cgtcaccgct atggcttaag ttgaaccata gcggcgtttc cggcgcgaat gcaggtttgc
    12481 cttgttcgcc atagatgatc tccggtagcg gggaaagcgt gtgcgaaagc aatgcacgcc
    12541 ccgccagcca gcgttcgcgt cgtggacctt gcggtgcttg ctcgcgtaaa cccggtggca
    12601 gtggagctgc gcttaaggtc gaaactttcc ccagaactat ccgatacata tcagggccaa
    12661 cgtttaatgg aaaatgaaag tgcgtatcgt atcacttgtc gcctcatccc ggtaaccgac
    12721 ttttcggtct gcctggcccc agtaaaatcg ccagtttgct accgcctttg gtggtttcca
    12781 tccagatttt acacacgctg gttaatggca ccgaaagtag catccctacc gggccgagca
    12841 gccatcccca aattaacaat gaaagaaata ccaccatggt ggacatcccc aggcgatggc
    12901 ccatcatccg tggttctaaa atattgccga tgaccatatg gaccactaaa aacaatgcgc
    12961 cgaccagaat acatgcgtaa acaccattaa acagcagcac ctgaatcatt ggcggtacgg
    13021 cggaaattac cgcgccgata ttgggcacgt agttgagcaa aaacgccagt actgcccaca
    13081 tcagcgcaaa ctgtacgccc attagcgcca gccccagcca gacgatgaca cctgtccata
    13141 aactgagtag cgtctttaac gccagataat gcgaaacgcc tttaagtgcg cggtgtaatc
    13201 ctgcgatgtg aatctgtggg ttattcagcg caaaacgcat tttgtaaggg acgtggcgca
    13261 cttcaaacag cataaaaact acggtcatca ccagcaaaag cacgctcgcc attgccccgg
    13321 aaagcccgat cattagcgct gtggtgaagg taaccacttt ttccgagtcc atccgctgca
    13381 gcattcgctc cggcgacata tgcaaattaa gaaaaggcaa catctcctgc aatttaaaaa
    13441 gtttgcgcgt cagctcctta ttaaacttcg gcagcataga gataaattcg ttaaacgatg
    13501 ccgccagtac gccgactagc gcggttagtg cgatcagcat caccaccact acaatcgtaa
    13561 tggtaacggg gcgttgtact ccccgacgaa taaaccaggt aacgagcggg ttgaggacga
    13621 tggcaaaaaa cagcgccagt aacagctgca caatgatatc tgccgctgcg tgaatgcccg
    13681 cgaggatcac taccagcgag gccaacttga gcagaatgtg catgcccgtt ttatcgggtt
    13741 gaggggtttc cattggggct tccttgtgac tttttgtgtt aagtgtagcg ggagtcacgt
    13801 cagcaatatt ctgattctta ctgctgattt tcacgtcagc acgtggtaaa aatgaaacac
    13861 tgttgtaaaa atgtggtgat cctcatgccc gaacccgtag ccgaacccgc gctaaacgga
    13921 ttgcgcctga atttgcgcat tgtctccatt gtcatgttta acttcgccag ctacctcacc
    13981 atcgggttgc cgctcgctgt attaccgggc tatgtccatg atgtgatggg ctttagcgct
    14041 ttctgggcag gattggttat cagcctgcaa tatttcgcca ccttgctgag ccgtcctcat
    14101 gccggacgtt acgccgattt gctgggcccc aaaaagattg tcgtcttcgg tttatgcggc
    14161 tgctttttga gcggtctggg atatctgacg gcaggattaa ccgccagtct gcccgtcatc
    14221 agcctgttat tactttgcct gggacgcgtg atccttggga ttgggcaaag ttttgccgga
    14281 acgggatcga ccctgtgggg cgttggcgtg gttggctcgc tgcatatcgg gcgagtgatt
    14341 tcgtggaacg gcattgtcac ttacggggcg atggcgatgg gtgcgccgtt aggcgtcgtg
    14401 ttttatcact ggggcggctt gcaggcgtta gcgttaatca ttatgggcgt ggcgctggtg
    14461 gccattttgt tggcgatccc gcgtccgacg gtaaaagcca gtaaaggcaa accgctgccg
    14521 tttcgcgcgg tgcttgggcg cgtctggctg tacggtatgg cactggcact ggcttctgcc
    14581 ggatttggcg tcatcgccac ctttatcacg ctgttttatg acgctaaaag ttgggacggt
    14641 gcggctttcg cgctgacgct gtttagctgt gcgtttgtcg gtacgcgttt gttattccct
    14701 aacggcatta accgtatcgg cggcttaaac gtagcgatga tttgctttag cgttgagata
    14761 atcggcctgc tactggttgg cgtggcgact atgccgtgga tggcgaaaat cggcgtctta
    14821 ctggcggggg cagggttttc gctggtgttc ccggcattag gtgtagtggc ggtaaaagcg
    14881 gttccgcagc aaaatcaggg ggcggcgctg gcaacttaca ccgtatttat ggatttatcg
    14941 cttggcgtga ccggaccact ggctgggctg gtgatgagct gggcgggcgt accggtgatt
    15001 tatctggcgg cggcgggact ggtcgcaatc gcgttattac tgacgtggcg attaaaaaaa
    15061 cggcctccgg aacacgtccc tgaggccgcc tcatcatctt aaaacttact gaataaccag
    15121 cgtattaatg atgttttctg cggtggtctg cgctttttgc tgatcgtcag cgggcagcgt
    15181 aatttgcatg gtcagcagtt gattacccac gttacccaga ataacggaag agtacgccgt
    15241 ctggcctttc gcggagataa tactgtctaa ctgctgcatt ttgtgacctt tcagctcaat
    15301 ggctttattg gttaccactt gcagctgcgg atcgcggcta cgttgctgat cttccagacg
    15361 cttcgccagc accgccagat cttctttcgg atcatcgccc atgatgacga tgactgcttt
    15421 ctgcccggtg gcgtcggacc agacatgcat gttattggcc tgcgttccca gcttaccgct
    15481 ctggtcggtc atatccgctg gcagcgagaa acttaacttg ccatcaagca ggttgacggg
    15541 attcccggta gcgttatttt ccgcgaccga accctgcgcc gtagcgttag tgtctttatc
    15601 atcacaggcc gcaagcccca taaccagcag gccaattccg acatatttaa ccagattgcg
    15661 cattgacttc ttcctttcga taaacggcca taacggctca ttcatccatc ttatcacaac
    15721 tctgataacg aacctttaac tcgcctgcaa agcgttgatt tcggatttat ctgccagtct
    15781 tttcaacagc atattgagta atacgccata cattggcagg aagaaaacga tactgattaa
    15841 cactttgaaa cagtaatcga ccagcgcgat ttccatccag tgttcagcca taaaggcatc
    15901 cgggctacgc cagaaggcaa tgaagaaaaa ggccagcgtg tcgctgacgt taccgaacag
    15961 tgtggacgct gtcggtgcca gccaccagcg gcgactctga cgcaggcggt taaaaacgtg
    16021 cacgtcgagg atctgtccca gcgcgtaggc catgaaactg gcggtggcga tacgggcgac
    16081 aaacaggttg aagtgggcga gtgcgccgaa tccctgccag gaacccatat agaatagcga
    16141 cgagatgacg taggagatca gcaacgccgg gatcatcacc gcaaagatga tacgacgggc
    16201 cagcggtgcg ccaaaaatac gcacggtcag gtcggtagca aggaaaataa acgggaagct
    16261 aaacgcgccc caggtggtat ggaaacccaa aatggagacg ggaagctgaa ccagatagtt
    16321 actggaggtg atcaccagca gatgaaataa cgataaccag aacaacgcct tatagcgttg
    16381 agtttgcgag aaaacgttca tattgtacct ttttgattaa ccattggggt gagggaaccc
    16441 aatacgtacg acacgtctgt taccttaaca taataacgtg cttacccttt tttcgagccg
    16501 ccgcatgata ctgctttacg ttgacaatgc aatggttaat tttcacgcaa tcgttaacct
    16561 ggtttgctta cggactcaca gggcgtaaac tagcgccgtt tttttatgtg atgagaagaa
    16621 aatgaccgat ctcttttcca gccctgacca cacactcgac gcacttggcc tgcgctgccc
    16681 ggaacccgta atgatggtgc gcaaaactgt gcgcaatatg cagcccggcg aaacgttgct
    16741 gattatcgcc gacgatccgg ccactacccg cgatattcct gggttttgta cctttatgga
    16801 acacgaactg gttgcgaaag agacggatgg actgccttat cgttatttga ttcgtaaagg
    16861 cggttgatgg gggctgattg gcttcgatgc cgccttttcc cctcacccta accctctccc
    16921 cagaggggcg aggggaccga tcgcgctcaa tgttgcgatc ggtttgcctt atctcctgcg
    16981 caacaatctt aacgcattcg ctgtcaccag caccgtcgca cccgtatctg ccagcaccgc
    17041 cagccatagt ccggtcatcc ctaaaagcgt ggtgacgagg aagatccctt tcagtcccag
    17101 cgcaatggtg atgttctggc ggatattggc gtgagtggcg cgtgccagtt caatcatttg
    17161 caccaggccg cgcaggtggt tatgggttaa tgctgcatca gcggtttcca gcgccacgtc
    17221 tgtgccactg cccattgcaa tcccaatggc ggcagctttc atcgctggcg cgtcgttaat
    17281 accgtcaccg accatcgcca gcggcgcatg ttgattcagc tcggtcaccg ctttgacttt
    17341 atattccggc aacaggcccg ctttaaattc cagccccagc tccccggcaa ttgccgccgc
    17401 tgcgcgtgga ttatcgccgg tgaggatcac ccctttgacg cccagcgcgt tcagttcact
    17461 gatggcagtt gcagcatcgg cgcgcagggt atcctgcaat gcaatgatac ccagcacgtc
    17521 atcattacgt actaccagca caaccgtttg cccggcgctt tccagttcat taatcaaacc
    17581 agcaaatgca tcagcgggat gtttcccggc agcgcatatc aacacgcgct caccgttaac
    17641 ctgcgcttca atgccagacc cgaccagcgc ccgctgtgat tcggcggcgg gaatggcgag
    17701 tgcagcaacc tgtgcttcgc gcacgatggc ctgcgccagt ggatgcgtcg cgctttgctc
    17761 gaccgccgcc gccagtgtca gcagttcaga ttcactaata cccgttgccg gatgaatcgc
    17821 ggtaacgcgc ggtttaccga cggtcagcgt accggtttta tcaaacgcca cctgagtaac
    17881 acgacctagt tgttccagcg ccgctccgcc tttaatcaac gccccacgac gcgctgctgc
    17941 cgccagcccg gaggtaatcg ccgcgggcgt ggagataact aacgcacacg ggcagccaat
    18001 cagcagcagc gtcaggcctt tataaatcca ctcctgccag ctggcggcaa acagcagcgg
    18061 tggcaccagc gtcaccagca gggcgacggc cataatcgct ggcgtataaa tacggctgaa
    18121 acggtcgata aaccgctcaa tgggggcgcg gcgctcttcg gcttcttcga tcagtttcag
    18181 aatccggtca atggcgctgg cacccggttc tgacagcact tccagcgtta ccagacggtc
    18241 tacgctggtg gctcctgcag gaactttatc gcccgtcgcg cgctccaccg gaatggattc
    18301 tccggtcagg gcgctttcat caaaactggc aaacggtgag agcagtttac cgtcggcagg
    18361 caaacgccca cctgcggcga cttcaatcac atcgccaggc cgcaggctgt taatcgccac
    18421 ctcttcccgc tcaccgttac gcaggcgcgt ggcggtttct ggtttcagcg ccattaacgc
    18481 gctaacgccc tgacgcgcgc ggctggcggc ccagccttcc agtcgttcac caatcaaaaa
    18541 cagcagcaac accatcgcag cttcagccgt tgcgccaata aacagtgcac caatagcggc
    18601 tacgctcatt aaggtttcaa tggcgaagta gctgccggat ttgatcagcc gtaatgcctg
    18661 atgagcaatc gggtacagcc caaccagcgt ggtcgcgata aacgccagtt gcccgaacgg
    18721 atgattgaac tgctccagac cccagctgat tgccatcatc acgattagtg taatcagcgg
    18781 cagattctct ttcaggcgcg atgcttgtgg ctcgtcggcg gcctgctcat cgcgcaggga
    18841 atagcccgct ttttgcaccg cagattcaac ttgtgcacga atgtcattgt cggcatcaac
    18901 caccagtttt tcggtggcga acaacacctg cacctgattc acacctgcaa gctggcgcac
    18961 ggcattttct accttgcgcg cacaggcggc gcagtccatg ccactgactt tccagctata
    19021 gcgggtgccg gagacgtttt cagagagcgt tggcgagctg gagcatgcgc cgtcgcagca
    19081 acagtcgttg gcgttctgta ccgtggttag cggtttgaac gcagcaaatt gaggggcttt
    19141 cttgccgtga ttgtcaggtg tcgacatggc atactccggt taagtttttt ctcattaacc
    19201 gaaggataca ctctggagtc gactccagag tcaagtttta tcagaaatac agtgagcgga
    19261 cgatgaggaa gtgaccggca aagtagcagg ccgcagcaat cgcgttatcc gcacggaagc
    19321 gtcggcgata gtggctcccc agccagacaa agttactgat aaacagcaac gaagcgccaa
    19381 caaacgcaga gagcgccgga gcggtcggac ggaagaacca cagttcacct gccagccaca
    19441 ccatcaccag cgtcatgccg ataaacgtgc agataggcca acgatactct tccagccgcg
    19501 tccagataat cgccagtaac agcgcaccca gaaccagcaa caccagtggc agaggccaga
    19561 agaaagagag cgtcatctga ctggcgaaat agatggtgta cagcaggtgc gagaggaaaa
    19621 acgcgccgat ggcgtacatc agacgttgac gtggcaacag ggttagcgca tcgcccagca
    19681 gtgaggcgca cagccctgcc agcaccagat agctaatggc gtcgaacatc ggcgcttgcc
    19741 aggccagtaa cagcaggaga agaagggtta acggtttaaa cacccagcgt tgccaggttg
    19801 gcccacgata cgatgcatcc acagatagcc atgcggaaag acagacagcg ataaacgacc
    19861 aaagcatctt agttccttga attcgttatt ttttcgtaag cggaatgcct acggtcttgc
    19921 ttccagttta gtggcgaggg tggacggatg acaacaccat aactagcagg gtatgcttat
    19981 ttccgcattt tccgatgagg gataaagatg agcaaaccac ctcttttctt tattgttatc
    20041 attggcttaa ttgtcgtcgc cgcatcgttt cgttttatgc aacagcgacg ggaaaaagct
    20101 gataatgata tggctccgct ccagcaaaag ctggtggtgg tgagcaacaa gcgggaaaaa
    20161 ccgattaacg atcgccgttc gcgccagcag gaagtgactc cggcaggcac cagtatgcgc
    20221 tatgaggcaa gcttcaaacc gcaaagcgga ggaatggagc agacgtttcg cctcgacgcc
    20281 cagcagtacc atgccctgac agtgggcgat aaaggtacgc tgagctataa aggaacgcgc
    20341 tttgttagct ttgttggcga acaataaggt tattttttaa ctttaaattt cttctgccag
    20401 accagcagtt caaacacgcc gaaaaggaaa atccgcacct tttcggcggt ggtcatctgc
    20461 gggccatctt tcggtaaagt ggatttcaat agcgccagct gcataccatg catcagcacg
    20521 gtaaaaatca gcgccacgtt aacgaaaata ttcagcgggc gcgggaaggg atgcaccaga
    20581 ttgaggatta aaaatcccca aacgcagagc attaacaaac gaccaatatt aatcagcatc
    20641 actttctcct tgtgcttcgc gttgatacag ccgataagcc acctgacccg ccactttttc
    20701 ccgatgtaat gaccagtttg ccggaacagt gggcagaccg ttttcgactt cgctttcgac
    20761 ataaatcagg gcttcgtcag ccagccagcc gttatcttcc agtaaattta tcgtctcttc
    20821 taacaagcca cggcggaacg gtggatcgac aaacacgata ttatgcggtg tacctttttg
    20881 cgccaggaat gacatcgcgt tgctgttcac cacgcgtgca ttgcctgctt ttagtgtcgc
    20941 cagattctta attaactgct gagaaaccgc gcgatccatc tcaatcaacg tggcccccgc
    21001 agcgtagcgc gataacgctt ccagtcccag cgcgccgctc ccggcgaagc aatccagaca
    21061 ttgggcgtca acaatgaccg gagccagcca gttaaacaat gtttcgcgta cgcggtcggt
    21121 ggtggggcgc agacctgggc tatcaggaac cgggagttta cggcctcgcc actgcccgcc
    21181 aataatgcgg atttggccgc tgccggaatg attcggtttt ttcatgataa attgctcaat
    21241 ccgccagata acacatactt gcaggcggtg atgtgaatta agttaagtga tagactattt
    21301 catcattttt ttagctgcta tgtacatagc gttaacgctg tgccatgaag caacagcgag
    21361 gagtgtagtc gcaaatggcg aaagaaaaaa aacgtggctt tttttcctgg ctgggctttg
    21421 gtcaaaaaga gcagaccccg gaaaaagaga cagaagttca gaatgaacaa ccggttgtag
    21481 aagaaatcgt tcaggcgcaa gagcctgcga aggcttctga acaagccgtc gaagagcaac
    21541 cgcaggcgca tactgaagcc gaggcggaaa cttttgctgc cgacgttgtg gaagtcactg
    21601 aacaggttgc tgaaagtgaa aaagcgcagc ctgaagcgga agtcgttgca cagccggaac
    21661 cggtcgtaga agaaacgccg gagccagtgg ctatcgaacg tgaagagctg ccgttgccgg
    21721 aagacgtcaa cgccgaagag gtttcgccag aagagtggca ggctgaagcg gaaaccgtag
    21781 agattgtcga agcggcggaa gaagaagcgg ctaaagaaga aatcaccgac gaagagctgg
    21841 aagcacaggc gctggctgcc gaagcggcag aagaggcggt gatggtggtt cctccggtag
    21901 aagagcagcc ggtggaagaa atcgctcagg agcaggaaaa accgaccaaa gaaggtttct
    21961 tcgcgcgcct gaaacgcagc ctgttaaaaa ccaaagaaaa tctcggttcc ggatttatca
    22021 gcctgttccg cggtaaaaaa atcgacgatg atctgtttga ggagctggaa gaacagctgt
    22081 tgatcgccga tgtgggcgtg gaaaccacac gtaaaattat caccaatctg acggaaggcg
    22141 catcccgcaa gcagcttcgt gatgccgagg cgctctatgg cctgctgaaa gaagagatgg
    22201 gcgagattct ggcgaaagtc gatgagccgc tgaatgttga aggtaaaacg ccgttcgtga
    22261 tcctgatggt gggcgtcaac ggtgtgggta aaactacgac gattggtaag ctggcacgtc
    22321 agtttgagct gcagggtaaa tcggtgatgc tggcggcggg cgatactttc cgtgcagcag
    22381 cggttgaaca gcttcaggtc tggggtcagc gcaacaatat tccggtgatt gcccagcata
    22441 ccggtgcgga ttccgcctct gttatcttcg acgccattca ggcagctaaa gcgcgtaata
    22501 tcgacgtcct gattgccgat accgccggac gcctgcagaa caaatcgcac ctgatggaag
    22561 agttgaagaa aatcgtccgc gtgatgaaga aactcgacgt tgaagcgccg catgaagtta
    22621 tgctgactat tgatgccagt accgggcaga atgcggtaag ccaggccaaa ctgttccacg
    22681 aagccgttgg cttaaccggc atcacgctaa cgaaactgga cggcacggcg aaaggcgggg
    22741 taattttctc ggtggctgac cagtttggta tccctatccg ctacattggt gtcggcgaac
    22801 gtattgagga tttgcgtccg tttaaggcgg acgactttat agaggcactt tttgcccgag
    22861 aggattaaca atgattcgct ttgaacatgt cagcaaggct tatctcggtg ggagacaggc
    22921 gctgcagggc gttacgttcc atatgcagcc gggtgagatg gcgtttctga ccggtcattc
    22981 cggcgcaggg aaaagtaccc tcctgaagct gatctgtggg attgagcggc ccagcgccgg
    23041 gaaaatctgg tttagcggcc atgacatcac gcgtctgaaa aaccgtgaag ttccgtttct
    23101 gcgccgccag attggcatga ttttccagga tcaccatcta ctgatggacc gtactgtcta
    23161 cgataacgtg gcgatcccgc tgattatcgc tggtgccagc ggtgacgata ttcgtcgccg
    23221 ggtgtcggcg gcactggata aagtcgggct actggacaaa gcgaagaact tccctattca
    23281 gctttccggc ggtgaacaac agcgtgttgg cattgcccgc gcggtggtga ataagcccgc
    23341 ggtactgctg gcggacgaac cgaccggtaa cctggacgac gcgttgtcgg aagggattct
    23401 acgtctgttt gaagagttta accgcgttgg ggtaaccgta ttgatggcaa cgcacgacat
    23461 caacctgatt tcacggcgtt cctatcgcat gctcaccctg agcgatggtc acttgcatgg
    23521 aggcgtgggc catgaataag cgcgatgcaa tcaatcatat tcggcagttt ggtgggcgtc
    23581 tggatcgctt ccgtaaatcg gtcggcggct caggcgacgg cggtcgtaac gcaccgaaac
    23641 gcgcgaaatc ctcgccaaaa ccggtaaatc gcaaaaccaa cgttttcaac gaacaggtgc
    23701 gctatgcctt ccacggcgca ttgcaggatc tgaaaagcaa accgttcgcc acgtttttaa
    23761 cggtgatggt tatcgccatc tccctgacgc tgcccagcgt ctgttatatg gtgtacaaaa
    23821 acgttaatca ggcggcgacg cagtattatc cgtcaccgca aatcactgtt tacttgcaaa
    23881 aaacgctgga cgatgacgcc gctgcgggcg tggtggcaca gttgcaggcc gagcaaggcg
    23941 tggagaaagt gaactatctt tctcgtgaag acgcactggg cgagttccgt aactggtctg
    24001 gttttggtgg tgcgctggat atgctggaag aaaacccgct tccggcagtg gcggtggtga
    24061 tcccgaaact cgattttcag gggacggaat cactcaatac gctgcgtgat cgtatcacgc
    24121 agattaacgg cattgacgaa gtgcggatgg atgacagctg gtttgcccgt ctggcggcgt
    24181 tgaccgggct ggtcgggcgc gtttcggcga tgatcggcgt gttgatggtg gcggcagtgt
    24241 tcctcgtcat cggcaacagc gtacgtctga gcatctttgc tcgccgtgac tccatcaacg
    24301 tgcagaaact gattggtgcg acggatggtt tcatccttcg cccgttcctg tatggtggcg
    24361 cactgctggg attttctggc gcattgttgt cattaatttt gtcagaaatt ctggtgctgc
    24421 gattgtcatc ggcggttgcg gaagtggcac aggttttcgg aacgaatttt gatatcaatg
    24481 gtttatcgtt cgatgaatgc ctgctattgc tgctggtatg ctcgatgatt ggctgggtgg
    24541 cagcgtggct tgccacggta caacatttac gccactttac gcctgaataa taaaagcgtg
    24601 ttatactctt tccctgcaat gggttccgta gcagggaaag agatcccgtt gtctcttccc
    24661 ggtatttcat ctctatgtca cattttgtgc gtaatttatt cacaagcttg cattgaactt
    24721 gtggataaaa tcacggtctg ataaaacagt gaatgataac ctcgttgctc ttaagctctg
    24781 gcacagttgt tgctaccact gaagcgccag aagatatcga ttgagaggat ttgaatgact
    24841 gacaaaatgc aaagtttagc tttagcccca gttggcaacc tggattccta catccgggca
    24901 gctaacgcgt ggccgatgtt gtcggctgac gaggagcggg cgctggctga aaagctgcat
    24961 taccatggcg atctggaagc agctaaaacg ctgatcctgt ctcacctgcg gtttgttgtt
    25021 catattgctc gtaattatgc gggctatggc ctgccacagg cggatttgat tcaggaaggt
    25081 aacatcggcc tgatgaaagc agtgcgccgt ttcaacccgg aagtgggtgt gcgcctggtc
    25141 tccttcgccg ttcactggat caaagcagag atccacgaat acgttctgcg taactggcgt
    25201 atcgtcaaag ttgcgaccac caaagcgcag cgcaaactgt tcttcaacct gcgtaaaact
    25261 aagcagcgtc tgggctggtt taaccaggat gaagtcgaaa tggtggcccg tgaactgggc
    25321 gtaaccagca aagacgttcg tgagatggaa tcacgtatgg cggcacagga catgaccttt
    25381 gatctgtctt ccgacgacga ttccgacagc cagccgatgg caccggtgct ctatctgcag
    25441 gataaatcat ctaactttgc cgacggcatc gaagatgata actgggaaga gcaggcggca
    25501 aaccgtctga ccgacgcgat gcaaggtctg gacgaacgca gccaggacat catccgcgcg
    25561 cgctggctgg acgaagacaa caagtccacg ttgcaggaac tggctgaccg ttacggtgtt
    25621 tccgctgaac gtgtacgcca gctggaaaag aacgcgatga aaaaactgcg cgctgctata
    25681 gaagcgtaat ttccgctatt aagcagagaa ccctggatga gagtccgggg tttttgtttt
    25741 ttgggcctct acaataatca attccccctc cggcaaaacg tcaatcccca cgcagattgt
    25801 taataaactg tcaaaatagc tattccaata tcataaaaat ggggtatgtt ttagcagagt
    25861 atgctgctaa agcacgggta gtcatgcata aaacgaaata aagtgctaaa tcacaacacc
    25921 acaacacacg taataaccag aagaatgggg attctcagga tgaatataaa gggtaaagcg
    25981 ttactggcag gatgtatcgc gctggcattc agcaatatgg ctctggcaga agatattaaa
    26041 gtcgcggtcg tgggcgcaat gtccggtccg gttgcgcagt acggtgacca ggagtttacc
    26101 ggcgcagagc aggcggttgc ggatatcaac gctaaaggcg gcattaaagg caacaaactg
    26161 caaatcgtaa aatatgacga tgcctgtgac ccgaaacagg cggttgcggt ggcgaacaaa
    26221 gtcgttaacg acggcattaa atatgtgatt ggtcacctct gttcttcatc aacgcagcct
    26281 gcgtctgata tctacgaaga cgaaggcatt ttaatgatca ccccagcggc aaccgcgccg
    26341 gagctgaccg cccgtggcta tcagctgatc ctgcgcacca ccggcctgga ctccgaccag
    26401 gggccgacgg cggcgaaata tattcttgag aaagtgaaac cgcagcgtat tgctatcgtt
    26461 cacgacaaac agcaatacgg cgaaggtctg gcgcgagcgg tgcaggacgg cctgaagaaa
    26521 ggcaatgcta acgtggtgtt ctttgatggt atcaccgccg gggaaaaaga tttctcaacg
    26581 ctggtggcgc gtctgaaaaa agagaatatc gacttcgttt actacggcgg ttatcacccg
    26641 gaaatggggc aaatcctgcg tcaggcacgc gcggcagggc tgaaaactca gtttatgggg
    26701 ccggaaggtg tggctaacgt ttcgctgtct aacattgcgg gcgaatcagc ggaagggctg
    26761 ctggtgacca agccgaagaa ctacgatcag gttccggcga acaaacccat tgttgacgcc
    26821 atcaaagcga aaaaacagga cccaagcggc gcattcgttt ggaccaccta cgccgcgctg
    26881 caatctttgc aggcgggcct gaatcagtct gacgatccgg ctgagatcgc caaatacctg
    26941 aaagcgaact ccgtggatac cgtgatgggt ccgctgacgt gggatgagaa aggtgctctg
    27001 aaaggctttg agttcggcgt atttgactgg cacgccaacg gcacggcgac cgatgcgaag
    27061 taatcattaa tcggcaactt tgggttgccg ccaaattgct aatatcgagt acgttgcttc
    27121 atgccggatg cggcgtaaac gccttatccg gcctacaaga tccaaagaaa
//
LOCUS       DAJTNN010000039        26510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTNN010000039 DAJTNN010000000
VERSION     DAJTNN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.039"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(245..1018)
                     /gene="hisP"
                     /locus_tag="OO150_001457"
     CDS             complement(245..1018)
                     /gene="hisP"
                     /locus_tag="OO150_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5515650.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEAHERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1026..1742)
                     /locus_tag="OO150_001458"
     CDS             complement(1026..1742)
                     /locus_tag="OO150_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5515651.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGIYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVLHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(1739..2425)
                     /gene="hisQ"
                     /locus_tag="OO150_001459"
     CDS             complement(1739..2425)
                     /gene="hisQ"
                     /locus_tag="OO150_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5515652.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2515..3297)
                     /gene="hisJ"
                     /locus_tag="OO150_001460"
     CDS             complement(2515..3297)
                     /gene="hisJ"
                     /locus_tag="OO150_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5515653.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3518..4300)
                     /gene="argT"
                     /locus_tag="OO150_001461"
     CDS             complement(3518..4300)
                     /gene="argT"
                     /locus_tag="OO150_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5515654.1"
                     /translation="MKKSILALSLLVGLSVAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4566..5135)
                     /gene="ubiX"
                     /locus_tag="OO150_001462"
     CDS             complement(4566..5135)
                     /gene="ubiX"
                     /locus_tag="OO150_001462"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5515655.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAISLPEDLFARWQGA"
     gene            complement(5230..6747)
                     /gene="purF"
                     /locus_tag="OO150_001463"
     CDS             complement(5230..6747)
                     /gene="purF"
                     /locus_tag="OO150_001463"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5515656.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQDYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(6784..7272)
                     /gene="cvpA"
                     /locus_tag="OO150_001464"
     CDS             complement(6784..7272)
                     /gene="cvpA"
                     /locus_tag="OO150_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5515657.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(7531..8193)
                     /gene="dedD"
                     /locus_tag="OO150_001465"
     CDS             complement(7531..8193)
                     /gene="dedD"
                     /locus_tag="OO150_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5515658.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8183..9451)
                     /gene="folC"
                     /locus_tag="OO150_001466"
     CDS             complement(8183..9451)
                     /gene="folC"
                     /locus_tag="OO150_001466"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5515659.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9521..10435)
                     /gene="accD"
                     /locus_tag="OO150_001467"
     CDS             complement(9521..10435)
                     /gene="accD"
                     /locus_tag="OO150_001467"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5515660.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVEMGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGSIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10591..11250)
                     /locus_tag="OO150_001468"
     CDS             complement(10591..11250)
                     /locus_tag="OO150_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5515661.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGMLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11333..12145)
                     /gene="truA"
                     /locus_tag="OO150_001469"
     CDS             complement(11333..12145)
                     /gene="truA"
                     /locus_tag="OO150_001469"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5515662.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPDDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12145..13158)
                     /locus_tag="OO150_001470"
     CDS             complement(12145..13158)
                     /locus_tag="OO150_001470"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5515663.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13224..14360)
                     /gene="pdxB"
                     /locus_tag="OO150_001471"
     CDS             complement(13224..14360)
                     /gene="pdxB"
                     /locus_tag="OO150_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5515664.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIAVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14459..15454
                     /gene="flk"
                     /locus_tag="OO150_001472"
     CDS             14459..15454
                     /gene="flk"
                     /locus_tag="OO150_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5515665.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLERLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15451..16629)
                     /locus_tag="OO150_001473"
     CDS             complement(15451..16629)
                     /locus_tag="OO150_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5515666.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16931..18151)
                     /gene="fabB"
                     /locus_tag="OO150_001474"
     CDS             complement(16931..18151)
                     /gene="fabB"
                     /locus_tag="OO150_001474"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5515667.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18310..20316
                     /gene="mnmC"
                     /locus_tag="OO150_001475"
     CDS             18310..20316
                     /gene="mnmC"
                     /locus_tag="OO150_001475"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5515668.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQQDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20437..20715)
                     /locus_tag="OO150_001476"
     CDS             complement(20437..20715)
                     /locus_tag="OO150_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5515669.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(20749..21297)
                     /gene="epmC"
                     /locus_tag="OO150_001477"
     CDS             complement(20749..21297)
                     /gene="epmC"
                     /locus_tag="OO150_001477"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5515670.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21297..22106)
                     /locus_tag="OO150_001478"
     CDS             complement(21297..22106)
                     /locus_tag="OO150_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5515671.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFAGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22106..22930)
                     /gene="mepA"
                     /locus_tag="OO150_001479"
     CDS             complement(22106..22930)
                     /gene="mepA"
                     /locus_tag="OO150_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5515672.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(22934..24019)
                     /gene="aroC"
                     /locus_tag="OO150_001480"
     CDS             complement(22934..24019)
                     /gene="aroC"
                     /locus_tag="OO150_001480"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5515673.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24054..24986)
                     /gene="prmB"
                     /locus_tag="OO150_001481"
     CDS             complement(24054..24986)
                     /gene="prmB"
                     /locus_tag="OO150_001481"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5515674.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25152..25703
                     /gene="smrB"
                     /locus_tag="OO150_001482"
     CDS             25152..25703
                     /gene="smrB"
                     /locus_tag="OO150_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5515675.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(25776..26489)
                     /locus_tag="OO150_001483"
     CDS             complement(25776..26489)
                     /locus_tag="OO150_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5515676.1"
                     /translation="MATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSR
                     VSMSACPGLEQYDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDD
                     DFPDYIGPTKVSSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMEN
                     GKLCNGNNFDERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDT
                     TSRQPIDSTCRFTYILNMY"
BASE COUNT         6528 a         7255 c         6698 g         6029 t
ORIGIN      
        1 agatttaaat accgcatcat gcggcgtgga agttgtcgat attgtcatgg cgttccgtca
       61 cccctcaaat ttaagatgac gcgacaataa ccacgattaa cacccaatgc agcaggcagt
      121 ttttatcttt ggacagcgaa ttcagaagta tttttagcgg actgcaactt ttacatttca
      181 gcggaagctg actcgcaaat tccccatgaa aatactccgg cagcgttagc caccggagag
      241 gaaattattt cagcgatccc ttaaggaact gttgcagacg agggctttgc gggttgccaa
      301 ataactgctc cggcgcgccc tcttcttcta ttttcccctg atggaggaaa atgacatgag
      361 tagaaacatg gcgagcaaag cccatttcgt gagtcactac caccatggtt ttcccctctt
      421 ctgccagttg ctgcataata cgcaacactt cgcctaccag ttcaggatcg agcgccgagg
      481 taggttcatc aaacagtaaa acttccggtt ccatcgccag cgcccgcgcg atagaaacac
      541 gctgttgctg accgccggaa agatgcaccg gatatttccc ctgcgcacgt tcgtctatcc
      601 cgacttttgc cagatacttg accgcccgct cgtgcgcttc ctgcttgctc aggcccaaca
      661 cctgaatcgg cgcttccatg acgttttcca gcaccgtcat atggctccag agattgaagt
      721 gctggaatac catcgtcagg cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt
      781 tgagttgacc gtctttgtcg cgcaccagat tgatcgtctg gccattgacc acgatcgacc
      841 cttcactcgg tttttcgagg aagttaatgc agcgcagaaa ggtacttttc cccgatcccg
      901 acgatccgat gatgcttatt acatctccgg cattcgcttg cagtgagacc cctttcagca
      961 cttcatgttc gccgtagcgt ttgtgcaaat cgataacgtt taatttattc tcggacatcg
     1021 tattctcagt gcgttgaaga aggtttcaca tgctgcaacc agcgtttttc cgctctgcga
     1081 aagaggctga tcaggacata agagatgatt aaatagagca ccgcggcaat gccgaaggcg
     1141 gtaaaaggtt gatacgtggc ggcgttaata tcgcgggcta ttttcagcag atccggcaca
     1201 gtggccgtaa acgccagcgc agtagagtgc agcatcagga tcacttcgtt gctgtatgcc
     1261 ggtaacgcaa tacgcagcgc agaaggcaaa ataatgcaac gatacatttt aaaagtcgag
     1321 aagccatagg ctctggcggc ttcaatttcc ccatgcagaa ccgaacggat tgccccagca
     1381 aaaatctcgg tagtgtaagc gcaggtgtta agcgtcagcg ccagcacggt acagttcagg
     1441 ccactgcgga agaaagcgtt aaggaattcg gttcccttaa caatctcaag cgtgtatatg
     1501 ccggaataga acaccagcaa ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat
     1561 aaccagattg gaaactggat gtatttatta ctggagacac gaccaatcgc cagaaacagc
     1621 gccaggactc cgcctatcac taccgacaaa ataagcagcc acagagtgat cgccacacca
     1681 gtaaagcgat aaccgtcggt ccacagcagc ggtttccagt attcatgtaa gatttcgatc
     1741 acaggtcagc cctcttcaca cccacggagt agcggcgctc aaggaacagc agcacaccat
     1801 tggaaacagt ggtgaaaacc aggtaaatca cgccacagac gatggcgaaa tagaacggtt
     1861 cccaggtact tttgcctgcc agttgggtgg ctttgaccac atcttccagg ccgagtaacg
     1921 aaaccagtgc ggtagatttg aggatcacct gccagttgtt gccaatgcct ggcagggcgt
     1981 aacgcatcat cgccggaaac atgatccgcc gaaacacttg cccacgagta aaaccgaacg
     2041 ccgtcgccgc ctctatatgt cctttcggca ctgccataaa agcaccacgg aacgtttcgg
     2101 taaaataagc accgtagata aaaccgagag tgataatacc agcgaccatc ggatcgatat
     2161 caatctgccc gacgcccatc gcctccgtca ccgtgtttag cgcaatctgc aaaccgaaga
     2221 aaatcagcag catcaacact aagtccggca cgccacgaat cagcgtggtg tacccttcga
     2281 aaatcagccc cgaaagccga ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga
     2341 ttacagcgag cactacagag ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa
     2401 taacacctga aaacccatat aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc
     2461 tcttgtctcc ctccggttac ccgaagggag cgggctttct ttcaccgatg gcgattagcc
     2521 accataaaca tcaaaatcga agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg
     2581 catttcggca aaggctttgt tcagtgcttc gcgcagttcg ttatcttctt tacgcaggcc
     2641 catgccggtc cctacgccaa acagtttttc atctttaaca gacgggccac cgaatttgta
     2701 atctttaccg acaggttgtt tgaggaaacc ttcgctggca gcgacctcat cctggaacgc
     2761 ggcatcaata cgtccggcag tcaggtcaga ataaatgttg tcctgcccct gatacgagac
     2821 gatttcaatg ccttttggtg cccaatgttc attaccgaac gtctcctggg tggtgccctg
     2881 caatacgcct acccgtttgc ctttcagcga ctcgactgtc ggctgaatgt cagaattttt
     2941 cgccaccacc aaacgagaat cggcagcgta cagtttgtcg gtgaaggcta tttcttgctg
     3001 acgtttttcc gtaatggaaa gcgatgacat gatggcgtca atcttcttcg cttttaagga
     3061 cgggattaac gcatccagcg gattttcgac aaacgtacat tgcgtattga tgcgtttgca
     3121 taattccttt gccagatcga tatcgaagcc aaccagttcg ccttgtgaat tttttgattc
     3181 aaatggcgca taggtcgggt cggtaccgat gcggatgttt tgcggaatcg cagcaaatgc
     3241 cgcagttgcg ctggagaagg ccagaaccag agagagcgat agcaccagtt ttttcataat
     3301 gtcctcaact gacagtcttt ttatagggaa tatttacagg tttgttacac ttatcgtgcc
     3361 ataaaattga ccatcaaggc aaaatatccc gccccgttag atgtattatt tttcattaaa
     3421 aatgcttaag tatgcatttc aatgcaccac gatagtgcaa caaacctagc gtgcaccatg
     3481 atgatcattc catcaggtac agcttcccag cgacgtatca gtcaccgtag acattaaagt
     3541 cgaaatactt tttcgccatc ttgtcgtagg tgccgtcctg acgcagctcg ccaagcgcct
     3601 tattgaaggc agccgtcagt tcagcatcat ctttacgtag ccctacaccg gtgccatcac
     3661 cgaagtattt tttgtctttt actgatgagc cagcaaaggc gaaatcttta ccagcaggtt
     3721 gcttgaggaa tccttcgctg gcagcaactt catcttgtaa cgcagcatcc agacgtcctg
     3781 cagccagatc ggaatagacc aaatcctggt tggcataggc caccacatcc acgcctttgc
     3841 tacgccaggt ctcgttagcg taagcttcct gggttgatcc ctgcagcaca ccaacatgtt
     3901 tacctttcag tgaatccagc gttggctgaa tcggtgaacc tttggcagca atcaaacgag
     3961 aatctgcggc gtacagcttg tcggagaagg caatctcctg ctgacgttta tcggtaatgg
     4021 aaagcgacga aataatggcg tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa
     4081 agtcactggc aacccaggta catttcacct gcatccgttt gcacatctcg ttaccgagat
     4141 cgatatcaaa gccaacaaaa tcacctttag catctttcga cgagaacggc gcataggtgg
     4201 tatcggttcc gatacgtacc gtctccggta gcgccgcata gctggaagcc gctacggaga
     4261 gaccgactaa caaagacaga gcgagaatcg acttcttcat acataaccct caagtgaaaa
     4321 tggctttttt atgttgtgta ttgtgttgtg tgtttgcagg ctctttcatg caggtcttat
     4381 gccatcttga cgacaacagt aacattcaac gttaaatatg ttaataagac gttgcattat
     4441 tgtcctgaag ttgaagatag caggtatagc ggttgaatcg cagcgtttcg attgaatggc
     4501 agaaacaaaa tgtcgaggat ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag
     4561 cggagttatg cgccctgcca gcgggcaaag agatcttcag gaaggcttat tgcaaactgg
     4621 tcaagaacac gattaaccgt ctgatttatc acatcatcaa gggattgcgg gcgatgataa
     4681 aacgccggaa caggaggcat aatcaccgca ccgatttctg ccgcctgagt cattaaacgc
     4741 agatggccta agtgcaatgg tgtttcacgc acgcagagca ccaacgggcg acgctctttc
     4801 agcaccacat ctgccgcacg ggtcagtaag ccatcagtat agctatggac aatgccggaa
     4861 agggttttga ttgaacaggg taaaatcacc atccccagcg tctggaaaga accggaagag
     4921 atgctggcgg aaatatcgcg cgcatcgtgc gtgacatcag ctaatgcctg cacttcgcgc
     4981 agggaaaaat ccgtttcgag ggataaggtc tggcgcgccg cctggctcat caccagatgc
     5041 gtttcgatat ctgtgacatc gcgcagaacc tgtaataagc gcacgccata aatcgcgccg
     5101 ctggcaccgc tgatgcctac aatgagtcgt ttcataaata gttgccctgt cagacttgca
     5161 ggcagacttt gcagtatttc ggcgggagtt gcaagtcagg gtgccagacc ggcaccctca
     5221 gcgaaggcat catccttcgt tatgcatttc gagattttcc acttcgttct gacgttgcac
     5281 tgctttggcg tcatcattac gtaacgtatc gaggaaatcg aggtagtcct gatcaacatc
     5341 tttggtgacg tagacgccgt tgaacaccga gcattcaaac tgctggatat ccggattttc
     5401 agcgcgaacg gcgtcgatca gatcgttcag atcctggaaa atcaacccgt cagcaccgat
     5461 gatctggcga atttcatcaa cttcgcgacc gtgagcgatc agttccgtgg cgctcggcat
     5521 atcaatacca taaacgttcg ggaagcgaat ttccggggcc gcagaagcga ggtacacttt
     5581 cttcgctccg gcttcgcgtg ccatctcgat aatctgctca gaagtggtgc cacggacgat
     5641 ggagtcgtcg accagcagga cgtttttatc gcggaactcg gcgcggttgg cgttcagttt
     5701 acggcgcacg gacttacgac gcagctgctg gcccggcatg atgaaggtgc ggccaacata
     5761 gcggttttta acgaagccct ggcggtacgg cttgcccaga atacgagcaa tttccagcgc
     5821 gatatcacac gaggtttccg ggatcgggat caccacgtcg atatccagat cttcccattc
     5881 gcgggcaatt ttctcaccca gtttcgtgcc catattcaca cgcgcgctgt aaacggaaat
     5941 tttgtcgata aacgagtccg ggcgggcaaa gtatacatac tcaaacaggc acggattgct
     6001 gaccggattg tcagcacatt gacgggtaaa caactgccct tcttcagtga tgtaaatcgc
     6061 ttcgcccggc gcgacgtcac gcaggaaatc aaagcccagc gtatcgagcg ctacgctttc
     6121 ggaagcgacc atatattctg tacggttctc gtcaatatca cgttttccca gtaccagcgg
     6181 acgaatcccg tttggatcgc ggaaagcaac cataccgtgg ccgataatca tcgccacaca
     6241 ggcatatgcg ccgcggatta agcggtttgt ggcagcaatg gcagcgaaaa tattgtcggc
     6301 ttccagcggg tagtggcgga agttgtccag ctcgctagcg aagatattaa gcagaatttc
     6361 cgagtcggaa gtggtgttga tgtggcggcg tttttcttca aacagttttt tacgcaactc
     6421 gtgagcgttg gtcagattgc cgttgtgggc aagcgtaatg ccatacgggg agttaacgta
     6481 aaacggctgc gcttcagagg cgctggagct gccagccgta gggtaacgca catgaccaat
     6541 gcccatattg ccctgcaaac gctgcatatg gcgagcttca aatacatcgc tcaccagccc
     6601 gttcgcttta cgcaaacgga agcagttatt ggcatctatg gtgatgatgc cggcggcatc
     6661 ctgaccgcga tgctgaagca ccgttaaggc atcataaatc gactggttaa ccggcataac
     6721 accggcgata ccgacaatac cgcacatacg tctttttcct cgttaagcca catctcagag
     6781 cacttacgct ctgggcaaga aacttgacga gctttgcaga taatcaaaaa accatctgat
     6841 gataaaactg aactgcggga tcagctgtga tttgctccag tcttcgcttt tcgacacccc
     6901 ggtaaaggag tcgagaaaga agagaatggc ggcaacaatc aacacaccgc gcaacgcacc
     6961 gaaacagacg cccagcaccc gatcggtgcc tgacaacccc gttttctcca ccaactggcc
     7021 tatcacgaag ttcacgatag caccaacgat cagggtagcg ataaacagta ccgcgatggc
     7081 aattccattt cgaaccagtt cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta
     7141 gtaatgactg gcaacaaaga aagcacaacc ccatgtcacc agcgataacg cttcacgaac
     7201 aaagccgcgg atcaggctaa ccagagagga aaaagcaatc accgcgatta tggcgtaatc
     7261 aatccagacc atatgtgtcc cacgatttta cgccctgtca tcctgttcgg ggcgcattct
     7321 aacagaaaaa gaaaacgttt gcgtagggat ttccttccca cgcatcaata aaaatggcgc
     7381 tgaaaaaata ttcaacgcca tcgacttttt atgcctttgc ggcatcgtgc aatgcgtgtc
     7441 ggatgcggcg taaacgcctt atccgaccta cggttctaac actgcccaag cctgataaga
     7501 cgcgccagcg tcgcatcagg caagactgta ttaattcggc gtatagccca ttaccacgcc
     7561 acttaagcca gaaagttgct tcaactcacc cagcgaacct ttcagcttat ctttcgaggc
     7621 atccggccca accagaatac gggtaatttt accctgcact ggcgtggatg gcgacgtata
     7681 aacccgataa ccggcaccgc gcagcttacc gacaatctca ttcactttat cggcattttt
     7741 cagcgcaccc agttgcacaa cataggcttt acccgttggc gctgcttttt cttccacgac
     7801 cggcttcggt tctggcttcg gcgcaggtgg tgcttcaacc ttcggtttag gcggctccac
     7861 cggtttcggc ttcggtgggg cgaccggtgc aggttccggt tcaaactcgg tgttattggc
     7921 tgcaatagtg gccggatcga gcgacggtgc tgcggcatca cctgcccgca cctcttccgc
     7981 tgcgccttcc ggcggctgcg tcggcaacgc ctgggtggcg gctggcatca tatcaggctc
     8041 atcgcgatcg cccgcttttg gaaccagcgg gatagccgca aactcatcct gataatgttt
     8101 tttctgcccg tccagcagcc ctggaagtac aatcaccccc agcgccacca gcacgatcgt
     8161 gcccactaac cgattctgaa acttacttgc caccgcttct cctcgcgtca atcacttcca
     8221 tgacatgtgc gaccgtgtgg aaagaaccac acaccagcac ggtgtcttcc gctttagcgt
     8281 ccgccattgc ggcatcccat gcctgcgcaa cgctatcaaa tgatttgccg ttacccaaat
     8341 gctcaagcag ttgttctgcc gtggcaccgc gcggcccttc cagtggcgca caataccagt
     8401 catcaaccac gcttttcaac caggccagag ttccggcaat atctttatca tgtagcatac
     8461 cgataaccgc cagcacgcgc ccgtttttcg gtagcgcttt catacgcccg gtgagatatt
     8521 ccgccgcatg tggattatgc gcgacatcaa aaataacgcg tggcgactcg ctcacaatct
     8581 ggaaacgtcc cggcaaaatt gcgctggcaa tcccgtcgcg aatggcattt tcactgactt
     8641 ccagcccgct ggcacgcagt gccgccagcg ctgttgcggc attcggttgc gggacaagcg
     8701 gcaacggcag attttccagc gtgccgtgag catcgctaaa cgcccagtca tgatcggtga
     8761 cggaatagtt ccactcaacg ccccgacgtt gtaacagtgc acctttttcc tgcgccacat
     8821 cagcaatggt agaaggcatt tccggctcac cgacaattgc cggttttgcg ctgcgaaaga
     8881 tgcctgcttt ctcgcgacca atactttcgc gatctggccc cagccagtcg gtatgatcca
     8941 gcgcaatact ggttactacc gcgacatcgg catcgacaat attggttgcg tccagacgac
     9001 cgcccagccc tacttccaga atcaccacgt caagttgtgc ctgtttgaac agccacaacg
     9061 ccgacagcgt accgtactcg aaataggtca gggaaatatc accgcgtgcc gattcaatct
     9121 ccgcaaaaga ggcggtgtgg gccgattccg gcaattcctg accctgcaca cgtacgcgct
     9181 cggtataacg caccagatgc ggcgaactgt agacgcccac tttgtaccct gccgccatca
     9241 gaatcgactc cagcgtacgg caggtggtgc ctttgccatt cgtacccgca acggtaaaca
     9301 caaatggcgc tggtttcaga acgccaagac gagccgcgac ctggctcacg cgctcaaggc
     9361 cgagatcgat agttttactg tgcaggtttt ccagataaga aagccacgaa gccagaggcg
     9421 acgcggcttg aggagtgcgt ttgataatca tggtatccgc tgattcgtta cggtgagaat
     9481 agcaaaaggg cagagccagt ggccctgccc ttatcagtta tcaggcctca ggttcctgat
     9541 ccggtaccgg gggtaccact acgccttcac gcggcgcttc aggattcggc gctggcagat
     9601 tcatcaactt cgccagaatg ctcgccagtt tcaggcgcat ttccggacga cggacgatca
     9661 tgtcgatcga gcctttctcg atcaggaatt cactgcgctg gaatcctagc ggcagttttt
     9721 cgcgaacggt ctgttcgata acacgcggac cggcaaagcc aattaacgct ttcggttcag
     9781 cgatgttgag atcgcccagc atggcgaaac ttgcagaaac accgcccatc gtcgggtcgg
     9841 tcagcacgga gatgtacggc aagccgcgct cctgcatttt tgccagtgcc gcagaggttt
     9901 tcgccatctg catcagcgac atcagtgctt cctgcatacg tgcgccacca gaggcggaga
     9961 agcagatcag cgggcagtta tcttccagcg cctgctcaac ggcacgcacg aaacgtgcac
    10021 ccacaacaga ccccattgaa ccgcccataa aggcgaactc gaatgccgca gcgacaaccg
    10081 gcattccata cagagtgcct ttcatcacca ccagcgcatc tttttcgccg gtttctttct
    10141 gcgcagaagc cagacggtct ttatacttct tggagtcacg aaacttcagc acatctttcg
    10201 gctcaagctc gctacccatc tccacaaggc ttccttcatc taacaggcta tgcaggcgat
    10261 tacgcgctgt catacgcatg tgatggtcac acttcggaca gacctcaaga ttacgttcca
    10321 gctcagcgcg gtataaaacc tgaccgcagc tatcacactt agtccacacc ccttcaggaa
    10381 tgctcgcctt gcgggtggga gtaatgttgc ttttaattcg ttcaatccag ctcattaggg
    10441 acctttctgt ctgaacctgg ttcgatgcca gttttatctt tggggacgca taatgccatt
    10501 tttgccccca acagaccatg aatgttgcac attaaaacat aacagcccga aactttggat
    10561 aaaaaagtgg tcgaaccgcg gagttacttt ttattttgcg gcgcgtgccg cagcgcgttt
    10621 gtgacgaata aattcgaaaa ccccaggcaa tattgaaaca aaaataatgc caacaatcat
    10681 cagcttaagg ttgctctgaa tgaaggggag catgccgaag aaataacccg cgtaggtaaa
    10741 aatcagtacc cacaacagtg cgccgatcac gttataagcg gcgaaatgac ggtacgacat
    10801 gtggcccatt cccgcaacaa acggcgcgaa cgttctgacg atcggtacaa aacgggcgag
    10861 aataatcgtt ttgccgccat gtctctcgta aaactgatga gtcttatcaa gataactgca
    10921 acgaaagatt ttcgaatccg ggttactgaa cagtttgtca ccaaataacc gtccaatggt
    10981 atagttcact gcatcaccga caatagcggc aatcaacata agcaccacca tcatatggac
    11041 attgagatcg ttagtttcca gcgacgccaa tgcaccagca acaaacagca gcgaatcacc
    11101 aggcaggaat ggcgttacca ccagccccgt ttcgcagaac agaatcaaaa acagaatggc
    11161 ataaacccag acgccgtact ccgcgaccag ctctgccaga tgcacgtcaa tgtgcaggat
    11221 aaaatcgatg aggaaataaa tcaggtccat atttgcctat gccttgtact cgtcatcttt
    11281 caggctgtaa ctacgttggc tacagcctga aatgttccga gcattattct cgttagtccg
    11341 ccagaaatag cgggcccatt ggcggttttg gaagatcata ccggtcaggg taatccaccg
    11401 cgaccagata cagcccttcc gcttttgccg ttgctgccgc cagcgttctg tcctttgccg
    11461 ccagcaactc tgctatccag ctctccggct ggttgtgggc accgacttcc atcaggctgc
    11521 cgacaatatt cctgaccata tgatgtacaa aggcattcgc tttgatatct accaccacat
    11581 aaggaccgtg acgcgtgaca ttaatatgca taacgttgcg ccacggggtt cgggactggc
    11641 actgcaccgc acggaacgag gtgaaatcat tctcgcccag caagcattgc gcagcccgat
    11701 gcatccgttc agcgtccagc ggttcgtaaa aatgggttac ccctttactc agtaccgccg
    11761 ggcgcagccg atgattgtag atgatgtagc gataacggcg agccgtggcg ctaaatcggg
    11821 catgaaaatc atcaggtaca gctttaaccc aacgcacggc gatgtcatca ggtaaattcg
    11881 catttacgcc caaggtccac gccgcgtctt tgcgctgcgc ggttgtttcg aaatgcacca
    11941 cctgcccggt accgtgtacc cctgcgtcag tacgcccggc gcagaagacg gtgatgggtt
    12001 cgttcgccac ctgggagagc gccttttcca gcttctcctg cacactgcgg acttcattct
    12061 gccgttgcca gccgtaatac ttactgccgt cgtactcaat gcccagcgca attttataaa
    12121 ctggcagttg ttgctggtcg gacattagta cagatactcc tgcaccagtt tctcggcgat
    12181 ttttactgcc atcagcgcgc cgccaaagcg aacgttatcg gccaccgacc agaactggac
    12241 ttgctccggc ataccgtagt cattacgcac gcagccaaca gaaagatgcg gagtacccga
    12301 agcatcccct acctgagtcg ggaattcgtt ctcttcagag agcacaatat cttcgccctg
    12361 agcaaacgca tcacgcgctt cttctgccgc cagcggacgc agtgcttcaa agttgaccat
    12421 ctgggcatga ccgtagaata ccggtgcctg gacgacgctt gccgaaatca tcagcccttc
    12481 gtcctgcagg attttgcgta cttcgtcaac gatacgacgt tcttcacgca cgctaccttc
    12541 gctatccggc aacaacggca gcatattgaa cgccagttga cgaccgaaga aatcttcttc
    12601 gtcaatcgga atgccgttga gcaatttcgc actctgcccc gctaacgcat cgaccgcttt
    12661 tttgccctgg gcggaggctg aaatcaggct ggtaacactg atacgcgaca aaccgccctg
    12721 atcgattaac ggtttcagtg ccgccagcag ctggctggtc agactgtctg gtacggcgat
    12781 gacattccgg ttgcggtaat cggtcagtac aaacgggttt acttccggca ccaccagcgg
    12841 tacgtcgggt tcgagagcaa acagtccgct gctgtcgatc accaggcaac ctgagttggt
    12901 cgcttcttca acccaggcag cggtagcttc tttgcctgcg acaaaaaatg ccagctgcgc
    12961 ctgcgtccag tcgaattcag cggcatcctg cacggtgatt gttttaccac caaagcgcag
    13021 ttgttcgcct gcgctttcgt tacgtgccag tgcataaatt tccccaaccg ggaactgacg
    13081 ttcagccagc gtttcaagca gggcttcgcc cacagcgcca gttgcgccca ggacggcaat
    13141 gttccagcct tcagacatgg tggtttactc cagaaatagc aaagctccct gccaatgtta
    13201 cagcagagag catgaagaag agattaacgt gccggatgat gaacggcgtt aaaacccagt
    13261 ttacacagca atgatgccgc actggcgtca tcacaaatta catacagaga tgaccattca
    13321 cggcgctcaa gatagttttt gcgcagttta tcgaactcac ccggtatccc ggcgacttta
    13381 cgcagcggtg cgtcatcgcg gcgcacatca tacaccaaat gcaccagcct tttcagcgtc
    13441 ggttgatcga gcgggccatg cagcgtaatg cgaccaaact ctggcgcggg cagtaatgta
    13501 tccagcgcaa cgtgctgttc atgcccaata aacttgctat aagcttcaaa cacttgcgta
    13561 gtaccgcgtg ctttaccttc cagggtataa cctgcgatat gcggcgtgcc gatatccact
    13621 tttgtcagca actccacgtt aagttccggt tcgccttccc agacatccag cactacgctt
    13681 aacttctggc cttcattcag gcaggtcagc aacgcagtat tatcgacgac tgcgccacgg
    13741 caggcgttaa tcagaatcgc tcccggcttc aggctacgga tcagtttttc atccgccaga
    13801 tgcagcgttt tgtacggccc atctttgaag agtggcgtat ggaaagtcag aatatccgca
    13861 tgctgaacta attcatccag cgagcggaaa tcaccctcat ccccacggtc agcgcgaggc
    13921 ggatcgcaaa gcaatgtttt aatccctaac gcttccagtc gcgcctgcaa tcgacgtcca
    13981 acgttaccaa cgcccacgat ccccaccgta cggtcgtgca gtgaaaatcc atcgcgttcg
    14041 gcaagcatca gtagtgagga gaaaacatat tccaccaccg caatcgcatt acagccaggt
    14101 gcagcggaaa aaccaattcc cgcctgcttc aaccatgctt catcgacatg gtcggtcccc
    14161 gcagtggcag tgccaacaaa tttaatgggt tttcctgcca gcaaagattc attcactttc
    14221 gtgaccgaac gcaccatcag cgcatccgcg tctgccagtt gagcgacggc gattggacgc
    14281 ccgggaaccg cggtcacctc acccaaacgg ctaaataagt cgcgggcata aggcatattt
    14341 tcatcaacaa ggattttcac gtttgtgtta cctgtatgag acgagagtta accggacaag
    14401 tgtgccataa tctcgcggcc aggcatactt gcgaagattt caggtataag gatacgtaat
    14461 gatacaacct atttccggcc ctcctcctgg gcaaccacca ggtcagggag ataacctgcc
    14521 gtctggcgcg ggcaatcagc ctttatccag tcagcaacgt acttcgctgg aaagattaat
    14581 gacgaaagtg acctcactga cgcaacagca aagagcagaa ctgtgggcgg gtatcaggca
    14641 cgatattggt ctgtcgggag attcaccgct gctttcgcgt cacttccctg ccgctgagca
    14701 taatctggcg caacgtctgc tggccgcgca aaaaagccat tctgcccgcc agcttttagc
    14761 gcaattaggg gagtatttac gtctggggaa taatcgtcag gcggtcacgg attatatccg
    14821 tcataacttt ggtcagacgc cgctgaatca gctctcaccg gagcaattaa aaaccattct
    14881 caccctgttg caggaaggga agatggttat tccgcaaccg cagcagcgcg aggcgaccga
    14941 ccgtccttta ttaccggcgg agcacaatgc gctaaaacag ctggtgacca aacttgcggc
    15001 ggcaacgggg gaacccagca aacagatctg gcaatcgatg ctggaacttt ccggggtgaa
    15061 agatggcgag ttaattccag cgaaactgtt taaccatctg gtgacctggc tacaggcgcg
    15121 tcagacgcta agccagcaaa atacgccgac gctggaatca ctacagatgg cgctaaaaca
    15181 acctttagat gccagtgaac tggcggcgtt atcggcatat atccagcaaa aatatggcct
    15241 ttctgcgcaa tcatcgcttt cttctgccca ggccgaggat attcttaatc agctttatca
    15301 acggcgggtt aaagggattg atccgcgtga tatgcaaccg ctgcttaatc cttttccacc
    15361 gatgatggac acgttgcaaa atatggcaac gcgtcccgcg ctgtggatac tgttagtcgc
    15421 gattatcctg atgctggtct ggctggttcg ttaaccccga cgaaatgaca gtatcgtgac
    15481 aataataccc agcaccgcag agatcgcccc ggcaagaaat accgaagagt aaccaaacgt
    15541 ggtcgccagc attcccgcca gcggcccgga gacgccgagg gcgatatcct gaaacgcggc
    15601 gtaaccgccc agtgcggtgc cgcgaacttg tgaggggacg cgtttaacca cctccacgcc
    15661 cagcgcagga aagataagcg aacatccggc tccggttaac gccgcgcccg ctaatgcgac
    15721 ccatgcacct ggggcttgcc agagcagcaa caagcccacc gtttctacaa gcagagagac
    15781 aatcgccact ttcacgccgc caaaacggtc cggcatccag ccaaacatga cgcgcatcac
    15841 gacaaatgcg ccgccaaacg cggtaagagt aaagcccgcc atcgcccatc ctttgctggc
    15901 aaagtagagc gaaacgaaag tcccgataac cgcaaaacca acgccttgta gtgccagccc
    15961 taaccctggt ttccagataa gcccgacaac gctccacagc gatggacgtt ctcccgccag
    16021 ggccggtact ttgcgcactg tgccgttaca ggcccacgcc agtaagggta ataccattgt
    16081 ggtgatcgcc agtgcggcaa aaccgtaatg gctatgaatc aacaggccaa gcggcgcacc
    16141 aacagcgagg gcaccgtaaa tcgccattcc gttccatgac atcactttgc cagagtgttt
    16201 tgcccctacg atgcctaatc cccaggtcag agcgcctgtc agtaactggc tttcaccaaa
    16261 gccaagaatc aaacgcccga cgaccaacag ggcaaatttc aacggtgcgg agacaggcaa
    16321 aatcgccgcc agcagcaacg cgccgccagc cagaccacaa gctaacatcc cctgaagcgc
    16381 cgaacgtttt gcaccatatt gatcggccag tcgcccggca tagccacgcg tcagcaccgt
    16441 agccagaaac tgaatcccga cggcaatccc gaccatggta ttgccatagc ccagatcatg
    16501 atgaacaaac agcgggataa ccggcaacgg cagccctacg gtcatgtagg tgagaaaaac
    16561 cgcaaaagcg atgcggaaga gcgaaaaatt ggcagaagat cgtgtttcgg tttggcttac
    16621 agcagtcatg cattactcca gaatgcagcg caaggcgagg agtatccccg tctcatctct
    16681 ctggtttcag ggttacagtg cgttggcagg atttaacgcg tacgtctttt cagaaggaaa
    16741 tcgacaaagc gggaagtttg cctggaactg gcggcgattg tcaatgatgt gaaaaaggga
    16801 accatcaggt tcccttttgc gttagtgccg gaggccgcat tggcgcgtaa cgtcggatgc
    16861 gacgctggcg cgtcttatcc gacctactcc gactactccg actactccga ctactccgac
    16921 ctactgcgaa ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg ccgccgaagc
    16981 cgaagctgtt agacataacg gtggtcagtt cgcgatcggt cgtttcggtc acgatgttca
    17041 gacctgccgc ctgctcgtcc agctcttcaa tgttgatgct cggggcgata aagccgtgtt
    17101 ccagcatcag cagagagtag atagcttcct gtacgccagc agcgcccaga gagtgaccgg
    17161 tcatggcttt ggttgcagaa atcgccgggc tcttatcgcc gaacacttca cggatagctg
    17221 ccagttcttt cacgtcgcca accggagtcg aagtaccatg agagttcagg taatcgattg
    17281 gggtatcaac gccgtgcatc gccatcttca tgcagcgtac tgcgccttcg ccagacggag
    17341 caaccatgtc tgcaccatca gaggttgcgc cgtagccaac gatttcagca tagatgtgag
    17401 caccacgcgc cagcgcgtgt tccagctctt caaccactac cataccgccg ccgccagcga
    17461 taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc tttttccggg gtgtcgttgt
    17521 atttagtaga cagcgcaccc atcgcgtcga attcgcaggc catttcccag cacagctctt
    17581 cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg gatctgctct actgcgttac
    17641 cgatacagtg tgcggaagtc gcacacgcgg agctgatgga gtagttaacg ccatgaattt
    17701 taaacggcgt ggcgaggcag gcagaaacgc cggatgccat cgctttggtg accacatacg
    17761 ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc agcgccgaat acctggaaac
    17821 gcggggaacc gccgccagaa cctgcaatca ggccaacgcg cgggttattc tggtaagctt
    17881 ccggagaaag gcccgcatct gcgattgcct gctccataga aaggaatgca taaatggatg
    17941 cgtcgctcat aaagcgcaca actttgcggt caatgaggcc agtggtatcc agttttacgt
    18001 tgccccagac gtggctacgc atgccggaat ccttaagctc ctgagagaaa gtgatccctg
    18061 aacgtccttc acgcagagat gccaggactt cctgctggtt attaccgatg ctggaaacaa
    18121 tgcccaggcc agtaatcact gcacgtttca ttcaatacct ctgtaagtcg cacatagagt
    18181 aagtttcgaa tgcacaatag cgtacacttg tacgccgaac aagtccgatc agccatttaa
    18241 tagagaaatt tgcgcagcct tacacacatc gctaagatcg agccaccgcc tgtaagacga
    18301 gtaacttacg tgaaacacta ctccatacaa cctgccaacc tcgaatttaa tgctgagggt
    18361 acacctgttt cccgagattt tgacgatgtc tatttttcca acgataacgg gctggaagag
    18421 acgcgttatg tttttctggg aggcaaccaa ttagaggtac gctttcctga gcatccacat
    18481 cctctgtttg tggtagcaga gagcggcttc ggcaccggat taaacttcct gacgctatgg
    18541 caggcatttg atcagtttcg cgaagcgcat ccgcaagcgc aattacaacg cttacatttc
    18601 attagttttg agaaatttcc cctcacccgt gcggatttag ccttagcgca tcaacactgg
    18661 ccggaactgg ctccgtgggc agaacaactt caggcgcagt ggccaatgcc cttgcccggt
    18721 tgccatcgtt tattgctcga tgaaggccgc gtgacgctgg atttatggtt tggcgatatt
    18781 aacgaactga ccagccaaca ggacgattcg ctaaatcaaa aagtagatgc ctggtttctg
    18841 gacggctttg cgccagcgaa aaacccggat atgtggacgc aaaatctgtt taacgccatg
    18901 gcaaggttgg cgcgtccggg cggcacgctg gcgacattta cgtctgccgg ttttgtccgc
    18961 cgcggtttgc aggacgccgg attcacgatg caaaaacgta agggctttgg gcgcaaacgg
    19021 gaaatgcttt gcggggtgat ggaacagaca ttaccgctcc cctgctccgc gccgtggttt
    19081 aaccgcacgg gcagcagcaa acgggaagcg gcgattatcg gcggtggtat tgcctgcgcg
    19141 ttgttgtcgc tggcgctatt acggcgcggc tggcaggtaa cgctttattg cgcggatgag
    19201 gcccccgcac tgggtgcttc cggcaatcgc cagggggcgc tgtatccgtt attaagcaaa
    19261 cacgatgagg cgctaaaccg ctttttctct aatgcgttta cttttgctcg tcggttttac
    19321 gaccaattac ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt aggctgggat
    19381 gagaaaagcc agcataaaat cgcacagatg ttgtcaatgg atttacccgc agaactggct
    19441 gtagccgttg aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa ttgcagcggc
    19501 attacttatc cgcaaggtgg ttggctgtgc ccagcagaac tgacccgtaa tgtgctggaa
    19561 ctggcgcaac agcagggttt gcagatttat tatcaatatc agttacagaa tttatcccgt
    19621 aaggatgact gttggttgtt gaattttgca ggagatcagc aagcaacaca cagcgtagtg
    19681 gtactggcga acgggcatca aatcagccga ttcagccaaa cgtcgactct cccggtgtat
    19741 tcggttgccg ggcaggtcag ccatattccg acaacgccgg aattggcaga gctgaagcag
    19801 gtgctgtgct atgacggtta tctcacgcca caaaatccgg cgaatcaaca tcattgtatt
    19861 ggtgccagtt atcatcgcgg cagcgaagat acggcgtaca gtgaggacga tcagcagcag
    19921 aatcgccagc ggttgattga ttgtttcccg caggcacagt gggcaaaaga ggttgatgtc
    19981 agtgataaag aggcgcgctg cggtgtgcgt tgtgccaccc gcgatcatct gccaatggta
    20041 ggcaatgttc ccgattatga ggcaacactc gtggaatatg cgtcgttggc ggagcagaaa
    20101 gatgaggcgg taagcgcgcc ggtttttgac gatctcttta tgtttgcggc tttaggttct
    20161 cgcggtttgt gttctgcccc gctgtgtgcc gagattctgg cggcgcagat gagcgacgaa
    20221 ccgattccga tggatgccag tacgctggcg gcgttaaacc cgaatcggtt atgggtgcgg
    20281 aaattgttga agggtaaagc ggttaaggcg gggtaatctg ctctcgcagt gtttgccgga
    20341 tgcggcgtga acgccttatc cgacctacgt gtgacgtgta ggcctgataa gacgcgataa
    20401 gcgtcgcatc aggcatggtg ctccagacgc cgcaacttac tgctgtgacg cctgctgaaa
    20461 caggttttcc cacatatcgg tcaccagcgc ctggtcacgc ggcgacaatt caccggcacc
    20521 aatggctttt tccagactct ggctaacggt cgtatgcacc gcctgagcgg agtggtcatc
    20581 accactttcc agttctgcga tggctaatgt caggtggcca cgcaaatacc cactggcaaa
    20641 caactcatca tcactggcat ggtcaatcat gccgtcgatt aatgccagaa tgcgtgattc
    20701 aaactccgcg atcatcttct ttcctcattg taaaacgtgg caccggcttc agttgagcgc
    20761 ttccggccac gggaactgtt ccgccgtaag ttccggcgtg tgataataat tttgtagtgc
    20821 tttaatgaag cgtgccggac gctcgggaat gccatttgtc agataatcca tcacctgcgc
    20881 atgtacccgc cgttgaaaca ccacacgatc cggttcgaag tcgccttcca gattgtcgca
    20941 actgacgtta aacggatatc ccgccgccac acagaacaac cagtcgagtg cctgcggctt
    21001 cacttcaaca tcttcaaact ggctttgcgt ttgggcatca cgcccatccg ggcaatacca
    21061 gtagccgaaa tcaaccagct cacggcgcgc ttttccggca atacaccagt gcgaaatctc
    21121 atgaattgcg ctggcataaa agccgtgggc aaagacaatt cggttatacg gtacttccgc
    21181 atcagcagga agatagatcg gttcgtcgtc gcctttaatc agacgggtat taaattcatc
    21241 ggcaaagcag ctattaaaaa tttcaatcaa ctgctcatag tggtgtgtac tgttcattag
    21301 ttcatcccca accagtggag gatctcctgt ccgtggctat cgtaaagtaa tttggcactc
    21361 atcaccgccg agacgataac gatcatcggg cggatcagtt tttgtccttt gctcagcacc
    21421 agtcttgaac ccatgcgcgc gccgaggaac tgccccacca gcatgacaaa gcccgtcgcc
    21481 caaatcactt tgccgccgag aataaacagc agcagaccgc caatgtttga cgttgcgttg
    21541 agtaatttgg cgtgagccgt ggctttggcg aggttgaatc cgcacagcgt aacgaaggcc
    21601 agcgcgtaaa acgatccggc agccgggcca aagaatccgt cataaaaacc gacgcagcca
    21661 ccggcaatta acgcgaaggg taaaccgtac atccggcgct ggcgatcttc ttcacccagc
    21721 tttggcatca gcaagaaata gaggccgata caaattacca gaatgggcaa aatctgccgt
    21781 aagacatcag cctgaacgta ttgcaccagc agtgcgccgc tcattgagcc ggcaaaggtc
    21841 atggcgatat tgagtttctg atcgcttaaa ctaaccactt tgcggcgaat aaagtagata
    21901 gtggcggaaa tagagccgcc gcaggcttgc agtttattgg ttgccagcgc gttagcggga
    21961 gacattcccg cagccatcaa tgccggaatg gtgagtaacc caccgccacc ggcaatcgag
    22021 tcgataaatc ccgccagcat ggcgacaaaa aagagaactc ccagcaacag cggggaaacc
    22081 ataaacaggc tattaaacgt ttccattaga tcacgtgctc atccagtagc gcctggcagg
    22141 aaggcggcaa cggaggcggt gtcttcttct caggctttgt tgttcctggt tttggaggtg
    22201 caaaccagct ttgcagttct gccccgcaac catcgcctgg tggcggtaaa ggttgatctt
    22261 cacactccag actatcggca gggcaacgta atcgtacatg catatgcgcg cgatgctgga
    22321 accagggtcg cactttgcgc aaccagtcgc gatcggtgcc cgcatcaagg caaagttgtt
    22381 gtttaatcgc cggattaaca aaaatgcgcg tgacgtcttt gtcctgggcg gcgagtttga
    22441 tcaagctgaa aatttctggc ttccacagcg tggagacaac gtgtttaccg tcgcgggaaa
    22501 ctaagtccag tgcttgcggg cgcaagagct gcgcggaggt ccagcgagtt ttcggcagtt
    22561 gcagaaagat atcgacatcc agtccggtct ggtggctggc atgaccgccg ttgaaacgcc
    22621 caccagcggg catccccata tcgccaatca gcaccgtacc catgcccaga ttgctcacct
    22681 ggctactcag acgctggata aacatcacca gatccgggtg accgaaatag cgacgctgat
    22741 cggtacgcat gacctgataa tgttcggact gtatcggcaa cgtgtcagcg ccgacaatac
    22801 agccattaga aaaactgcct atcgattgtg cgctacccgg cacaggttgg gttatttttt
    22861 gccacggcgt cgctgccaag ctagcgctac tggcaagcag agccagcagc gcaatcgcgg
    22921 ttttgttcat tttttaccag cgtggaatat cagtcttcac atcggcattt tgcgcccgct
    22981 gccgtaacag gtgatccatt aaaacgatcg ccagcatcgc ttcagcgatc ggcactgcac
    23041 ggatcccgac acagggatcg tgacgacctt tggtgatcat ctcaacttct tcgccaaagc
    23101 ggttaatagt gcgccccggc acggtaatgc tggaggtcgg tttcagcgcc atatgagcaa
    23161 tgatttgctg cccgctgctg ataccgccga gaatgccgcc cgcatggttg ctctggaaac
    23221 cgtctttggt gatttcgtcg cggttctggc tgccgcgcag cgccactacg tcaaagccgt
    23281 cgccaatttc cacgcctttc accgcgttga tgctcatcag cgcatgggcg atgtcggcat
    23341 ccaggcggtc aaataccggc tcgccaagtc cggcggggac gccactggca acaacggtga
    23401 ctttcgcgcc gatggagtcg ccctcttttt tcagcgcgcg catcagttca tctaacgcgt
    23461 cgattttgtc cggatccggg cagaagaacg gattttgttc aaccagcgac cagtctttga
    23521 tttccagcgg aatgtcgccc atctgggtca agcagccgcg gatttcaata ccaaatttct
    23581 cggcgagata ttttttggca atcgcccctg ccgccacgcg catggcggtt tcgcgggcgg
    23641 aagaacgtcc accgccgcga taatcgcgca gaccgtattt ttgttcgtag gtgtaatcag
    23701 catggcctgg gcggaaaacg tctttaattg cgctgtaatc ctgagagcgc tggtcagtgt
    23761 tttcgatcaa caagccaatg ctggtgccag tagtaacgcc ttcaaaaaca ccagagagaa
    23821 ttttgacctg atccggctcg cggcgctggg tggtatagcg cgatgtccca gggcgacgac
    23881 ggtcgaggtc atgttgcagg tccgcttccg tcagcagaat gcctggcgga acaccatcga
    23941 cgatgcagcc gagcgccagc ccatgcgatt cgccgaaggt ggttacgcga aagagttgtc
    24001 caattgtgtt tccagccatc acggctccgt tattgttgtg tttgcgtgtt tacttaatct
    24061 ttataaatcg cgaaatattc acgggccgcc agcagctgct ctttggtgag cataaacaca
    24121 ccatcgccgc cgttatcaaa ctccagccag gtgaacggaa catccggata ttgttccata
    24181 agatgtacca tgctgttgcc gacttcacaa atcaacacgc catcatcagc aaggtaatct
    24241 gccgcgtttc caagaatgcg acgcgtcagt ttaaggccgt cggtgccaga tgccaggccc
    24301 agttccggct cgtgacggta ttcgtttggc aggtcggaca tatcttccgc gtcgacatac
    24361 ggcgggttag tgacaatcag atcgtactgc actttcggca agtcgcggaa cagatcggaa
    24421 cgaatcggaa tgacgttgtg gatcagaccg tgttcttcga tgttctgttc agcaaccgcc
    24481 agcgcgtcgg gagagatatc caccgcgtcg acttctgcat ccgggaaggc ataagcacag
    24541 gcaatggcga tgcagccgct accggtacac atatctaaaa tatgctgcgg ttgtttgctg
    24601 atgagtccgg caaatttatt gttgatcagt tcaccaatcg gcgagcgcgg caccagcacg
    24661 cgttcatcga cgtaaaattc atgaccgcag aaccaggctt tgttggtcag gtaagccacc
    24721 ggaatgcgct cgttgacgcg gcggatcacg cgttcaacaa tacgatgttt ttcgctggag
    24781 gtcagacgcg cggtgcgcat atcttccggg atatccagcg gcaggtagag cgatggtaac
    24841 accagctgta cggcttcatc ccacgggtta tcggtgccgt gaccgtacca gatatttgcc
    24901 gcgctgaagc ggctcactga ccagcgcaac atgtcctgaa tggtttgcag ctcatttact
    24961 gcttcatcaa cgaaaatttt atccacgtat tcctccaggg catgatcgca ataatttcgg
    25021 cggctagttt gccatgaaga tgacgataaa tcagcattca cgcgcggtga gtgaggaaaa
    25081 atacgtttaa aacgatcgat tgtgctacga gtcgggtaaa ctgtaggaaa attagaaata
    25141 gagacagata aatgaaaaag aaaacaacac ttagcgagga ggaccaggct ctgtttcgcc
    25201 agttgatggc ggggactcgc aagattaagc aggacacgat tgtccaccga ccgcagcgta
    25261 aaaaaatcag cgaagtgccg gtgaaacgct tgatccagga gcaggctgat gccagccatt
    25321 atttctccga tgagtttcag ccgttattaa ataccgaagg tccggtgaaa tatgttcgcc
    25381 cggatgtcag ccatttcgag gcgaagaaac tgcgccgcgg cgattattca ccggaattgt
    25441 ttttggattt acacggtctg acgcagctgc aggccaagca ggaactgggc gcgttgattg
    25501 ccgcctgtcg ccgcgaacat gtgttttgtg cctgcgtgat gcatgggcac gggaagcata
    25561 ttttgaagca acaaacaccg ctgtggctgg cacaacatcc gcatgtgatg gcatttcacc
    25621 aggcaccgaa agagtacggc ggtgatgcgg ctttgttggt gttgattgaa gtggaagagt
    25681 ggttgccgcc ggagttgccc tgaggagttg agcgtgttgt gccaggagcg cggaatggca
    25741 ggggcgaatg accctgccag gtgagaaaag gtgcgctaat acatattcag tatgtaagtg
    25801 aatcggcagg tagaatctat gggctgtcga gatgtcgtgt ccacgcgtag cttcatatca
    25861 tgcagttgcc tggacgtaat cggttgcggc tcaggcgtca ccgtcacttt ggcattatca
    25921 cagccggaag tcgagaaggt catctgctgt gcgataaaac ggcagtagac accacgctca
    25981 tcaaaattat ttccgttaca cagtttgcca ttttccatca ggaaagtggg cagtgtttgc
    26041 tcaataacac cacctgttga ctgcaacctg acgactttgt tatgtacaac ataattttca
    26101 ttccaggaaa catcatacgg tgccaggggc accgccggac atgaagaact caccttcgtt
    26161 gggccaatat agtcaggaaa atcatcatca tacgttttag cgaagggatc tctactctta
    26221 acaaacgacg acacaatcca cggacaacca tgtaaattcc catcgtaata aacaaacatc
    26281 tttaattcat tacatgtcca tcccgttttg gtctggctat catactgctc taatccagga
    26341 caggctgaca tggataccct gctgtccgaa attttcgaca tagatggttg agtagaacta
    26401 tcgccagtcc acgaaactaa atccggctgt gaattgggca tgaatgtagc cttaaatata
    26461 gcgtaattaa ctgaatctgg cgtcgctata atcagatgaa actcaaactc
//
LOCUS       DAJTNN010000040        25620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTNN010000040 DAJTNN010000000
VERSION     DAJTNN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.040"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..2115)
                     /locus_tag="OO150_001484"
     CDS             complement(<1..2115)
                     /locus_tag="OO150_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5515677.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSSEEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVLHPAYGNVRIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMA"
     gene            complement(2226..3638)
                     /gene="ftsP"
                     /locus_tag="OO150_001485"
     CDS             complement(2226..3638)
                     /gene="ftsP"
                     /locus_tag="OO150_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5515678.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDSRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAASPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3713..4450)
                     /gene="plsC"
                     /locus_tag="OO150_001486"
                     /gene_synonym="parF"
     CDS             complement(3713..4450)
                     /gene="plsC"
                     /locus_tag="OO150_001486"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5515679.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4683..6941)
                     /gene="parC"
                     /locus_tag="OO150_001487"
     CDS             complement(4683..6941)
                     /gene="parC"
                     /locus_tag="OO150_001487"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5515680.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSLMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(7487..7969)
                     /locus_tag="OO150_001488"
     CDS             complement(7487..7969)
                     /locus_tag="OO150_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5515681.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(8022..8414)
                     /gene="ygiW"
                     /locus_tag="OO150_001489"
     CDS             complement(8022..8414)
                     /gene="ygiW"
                     /locus_tag="OO150_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5515682.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8566..9225
                     /gene="qseB"
                     /locus_tag="OO150_001490"
     CDS             8566..9225
                     /gene="qseB"
                     /locus_tag="OO150_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5515683.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLASEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9222..10571
                     /gene="qseC"
                     /locus_tag="OO150_001491"
     CDS             9222..10571
                     /gene="qseC"
                     /locus_tag="OO150_001491"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5515684.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPSGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            10681..11262
                     /gene="mdaB"
                     /locus_tag="OO150_001492"
     CDS             10681..11262
                     /gene="mdaB"
                     /locus_tag="OO150_001492"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5515685.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11293..11607
                     /locus_tag="OO150_001493"
     CDS             11293..11607
                     /locus_tag="OO150_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5515686.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11655..13547)
                     /gene="parE"
                     /locus_tag="OO150_001494"
     CDS             complement(11655..13547)
                     /gene="parE"
                     /locus_tag="OO150_001494"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5515687.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13576..14157)
                     /gene="yqiA"
                     /locus_tag="OO150_001495"
     CDS             complement(13576..14157)
                     /gene="yqiA"
                     /locus_tag="OO150_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5515688.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14157..14984)
                     /gene="cpdA"
                     /locus_tag="OO150_001496"
     CDS             complement(14157..14984)
                     /gene="cpdA"
                     /locus_tag="OO150_001496"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5515689.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(15009..15431)
                     /locus_tag="OO150_001497"
     CDS             complement(15009..15431)
                     /locus_tag="OO150_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5515690.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15432..16061)
                     /gene="nudF"
                     /locus_tag="OO150_001498"
     CDS             complement(15432..16061)
                     /gene="nudF"
                     /locus_tag="OO150_001498"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5515691.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHSLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16266..17747
                     /gene="tolC"
                     /locus_tag="OO150_001499"
     CDS             16266..17747
                     /gene="tolC"
                     /locus_tag="OO150_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5515692.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            17895..18566
                     /locus_tag="OO150_001500"
     CDS             17895..18566
                     /locus_tag="OO150_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5515693.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18572..19732
                     /locus_tag="OO150_001501"
     CDS             18572..19732
                     /locus_tag="OO150_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5515694.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLPLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19770..20558)
                     /gene="ygiD"
                     /locus_tag="OO150_001502"
     CDS             complement(19770..20558)
                     /gene="ygiD"
                     /locus_tag="OO150_001502"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5515695.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEARGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20701..21462
                     /gene="zupT"
                     /locus_tag="OO150_001503"
     CDS             20701..21462
                     /gene="zupT"
                     /locus_tag="OO150_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5515696.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGISPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21520..21690)
                     /locus_tag="OO150_001504"
     CDS             complement(21520..21690)
                     /locus_tag="OO150_001504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515697.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(21950..22603)
                     /gene="ribB"
                     /locus_tag="OO150_001505"
     CDS             complement(21950..22603)
                     /gene="ribB"
                     /locus_tag="OO150_001505"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5515698.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(22707..22855)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            22977..23267
                     /gene="ubiK"
                     /locus_tag="OO150_001506"
     CDS             22977..23267
                     /gene="ubiK"
                     /locus_tag="OO150_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5515699.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            23551..24102
                     /locus_tag="OO150_001507"
     CDS             23551..24102
                     /locus_tag="OO150_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR5515700.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILNSQSGEVTLGTPVVITFNNTNSYQELNFKVRM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            24162..>25620
                     /locus_tag="OO150_001508"
     CDS             24162..>25620
                     /locus_tag="OO150_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5515701.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNAIINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLHGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDTQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTN"
BASE COUNT         6416 a         6752 c         6328 g         6124 t
ORIGIN      
        1 cgccatcggc gtatgtttcg tcaacgccgg acgggtattg cggttctggc gacgagcttc
       61 acgcatctct tcaatggttg gcgcaggcac taagcaatcg cgacggctac caatcagatg
      121 ctttttaccc atcgcttcca gcgcctggcg gattaacggc cagtttgccg gatcgtggta
      181 acgcaacaac gctttatgca aacgacgctg cttgtcgccc ttcggtacga agacgtcttc
      241 actcttataa ccaatcttcg ccagcggttt tttcccggtg taatacatgg tggttgagtt
      301 cgccagcggc gatgggtaga agttctgtac ctggtcgaga cggaaacgat gctttttcag
      361 ccacagcgcc agattcacca tatcttcatc acgcgtaccg gggtgcgcgg agatgaaata
      421 ggggatcaga tactgctctt tacctgcctg ttttgagtaa gtatcgaaca gctctttaaa
      481 gcggtcatag ctgcccatgc ccggcttcat catcttcgat aacggccctt cttcggtatg
      541 ttccggggca atcttcagat aaccgccgac gtgatgggtc gccagttctt tgatatagcg
      601 cggatcttcc acggcgatgt cataacgcac accagaggca atcaggatct ttttaatgcc
      661 tttcagatca cgcgcacggc gatagaggtt gatcgttggt tcgtggttcg tgtccatgtg
      721 cggacaaata tccggataaa cgcacgacaa acggcggcag gtttgctcag cgcgtggcga
      781 tttgcagcgc aacatataca tgttggcagt tggcccaccg agatcggaaa ttacgcccgt
      841 aaaacctgga acggtgtcgc ggatcgcttc gatctcatta atgatcgaat cttcggaacg
      901 gctctgaata atgcgccctt cgtgctcggt gatagaacag aaagagcagc cgccaaagca
      961 gccacgcata atgttgaccg aaaaacggat catttcgtaa gccggaatac ggacattgcc
     1021 ataggccgga tgtagcacgc gcttgtatgg cagcgcaaaa acgctgtcca tctcttcggt
     1081 agaaagcgga atagcaggcg ggttgatcca cacatagcgg tcgccgtgtt tttgcatcaa
     1141 tgcgcgggca cagcctgggt tggtttcgtg gtgcagaata cgcgaagcat gggcgtacag
     1201 cactttatcg cccttcactt tctcgaaaga aggcagcaac acgtaggttt tttcccacgg
     1261 tttcgggcgc ggtggctgca cggttacggc tttggcttcc tgctttttcg gtgccaccgg
     1321 tttgttatcc gcgcacggca aatcttcacc atacggatgc gggattgggt cgatttttcc
     1381 aggggtatca agacgggtgg aatccacgcc gctccagcca ggcagggcct ctttcacgat
     1441 aatcgcggta ttacgcacat cgcggatttc actaatcggc tcgcccatcg ccagacggtg
     1501 cgccacctcc accagcggac gctcaccgtt accaaacatc agcatgtcgg ctttcgaatc
     1561 caccagcacg gaacggcgca cggtatcgga ccagtaatca taatgtgcgg tacgacgcag
     1621 actagcctca ataccgccga ggatcaccgg tacatctttc cacgcctctt tacaacgctg
     1681 ggtataaacc agtgtggcgc gatccgggcg cttacccgcg acgttatccg gcgtgtaggc
     1741 atcgtcatga cgtaaacggc gatcggcggt ataacggttg atcatcgaat ccatgttgcc
     1801 agcagtaaca ccgaaaaaca gattcggttt acccagacgc ataaagtcgt ctttgctgct
     1861 ccagtccggc tgggcgatga tcccgacgcg aaagccctgc gcttccagca tacgaccgca
     1921 aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg ccagtaacca aaatgatgtc
     1981 gcagctatcc cagccaagtt gatccatctc ttcactagac atcggcaaaa acggtgccgg
     2041 tccaaaacag gcggcccagt actgcggcca ggagaacagg tcgcgatccg gttggatcag
     2101 ggagatagag ctcattttgc ttccagaaat gataaaaaaa taatcaaagg ccggggatta
     2161 taagccggaa cgaaagagaa atcgaaaagt attccatact cgccctcctc gggcgagtat
     2221 gaagattacg gtaccggatt gactaacagt tgcccaatcg agccacggtc cgccatttcc
     2281 agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc gaaataaaca
     2341 agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc ttccgggaac
     2401 ggcatcgcac cattcacgtt acggatctgg aacatcacgc cttcaatatg gaacgcttgc
     2461 ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt gacatcaata
     2521 cggttgacgt cccacagttg tccattaata cccgggtcat cacccagact gatatcgcga
     2581 ctgcgaatcg gcgaagcggc catgatttca gttggcagca agcgcatcgg aagactgtct
     2641 gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccagggtaga aaccagaatg
     2701 ctggatggct caaagaagcc acgaatacga tcaacaatgc tcgccgcttc gccacaggtg
     2761 atcgacactt catcaccgtt gctcatatcc accagaatct cgcggcgctc gcccggtgcc
     2821 aacgaaagtt gcttcaccga tacaggagca ggcaggaatc cctgatcgcc agaaatcaca
     2881 tgtaacgggc gactatcgct catctgtagt tgatagcgac gagagttcga cgcgttcagc
     2941 agtcgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac accgttaacc
     3001 agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg cgtaccaaag
     3061 ttatccagcc gtttatcctg gataatgacc ggaaaatcat ccacaccata atggttgggg
     3121 ataggcagcg acttgctgac ttcatcttcc accagccaca ttccggcaag gccgttatag
     3181 acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc tgcgttctgg
     3241 cgaatgggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc
     3301 atcagcgggc ctggtacctg tagcccggcc accgtcattg agacattttc tgtcaggcgg
     3361 ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa
     3421 cgaccattga ttccccagac cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt
     3481 tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gtagcggcgg aacgggtagc
     3541 ggttgctgtt gcccggctgc gctggccttc aggggaacag cgccggcaca aagtgcaatc
     3601 cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg taaaactggc
     3661 taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg acttaaactt
     3721 ttccggcggc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt tgttccatta
     3781 tcgaacgaca atgggcagcc agctcacgaa cctgatcttt gccatactga ctgacgtcaa
     3841 ttggcggcag catttcgaca atcaccagac cgttgtgcag tcgattaaga ttaatcttat
     3901 tcgaagttgt agagacgcac acgggaataa tcgggacgcc cgccgcaatt gccgcgtgaa
     3961 atgctccagt cttgaacggt agcaggccgc gaccacggct gcgggttcct tccgggaaca
     4021 tccagatgga aatacggcgt tttttgaaat gattcacgac ttccgcaatg gtgccgtgag
     4081 ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac aactgcccga
     4141 agaaggggat ccacagcaag ctctttttac ctaccgtcac cgtcggcggt tgcacaatgt
     4201 tcgatgctgt caccatgtca tagttgttct ggtggttagc gatatagata gcattgccgt
     4261 agctttcagc atctgcaggt ttgcggcact caactttcag gccgaacagc ggcgcaagac
     4321 ggccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg aaaaggcagt
     4381 aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata agacgaaaga
     4441 tatatagcat aacaacctct gaaggtctga gagactggca ttgtacgtca cctgaaatca
     4501 ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg caacgcatga
     4561 ataaacaacg gtttcaaggg aatttttatc gtttccgggg gttgaatacg ctgccggatg
     4621 acgacttaac gtttcatccg gcgttccttg caagcgggag gaaacaggcc ctccccggca
     4681 tattactctt cgctatcacc gctgctggca cggcgaggag agtcgatctc aacacgatcg
     4741 atacgctgca aaccgcgcat caacgtaccg cggcgtccac gttcgccagt gactttctgt
     4801 aactcttccg ggcgcagttt aattttgcgt ttcccaacat gaatggtcag cgtgctttgc
     4861 ggcggcagaa cgtacaattg cgccagacca tcttctccac gcgcggcttc tgccgatgga
     4921 atgttgataa tcttgttgcc tttgcccttc gacagctgcg gcagatcgct taccgggaac
     4981 atcaacatac ggcctgcctg agtgattgcc agcagcatat cggaagcatc ttcaatcacc
     5041 accggcggca taacatgggc attttccggt aaggtgatca aagccttacc tgcacggtta
     5101 cgcgccacca gatcgttaaa ggtgcagacg aaaccgtaac ccgcatcgga agccatcagc
     5161 agtttctgat cgtcgctttc catcagcata tggtcaacgg tcgccccagg cggcaacgtt
     5221 aatttgccgg tgagtggttc gccctgacca cgcgccgacg gcagcgtaat cgggtcgatg
     5281 gcatagctac gaccggtgga atcaacaaac actaccggtt ggttgctctt acctttcacc
     5341 gccgctttga agctatcacc cgctttataa ttcaggcccg gcgcgtcgat atcatggcct
     5401 ttagcgctgc gtacccagcc catcagcgac agcacaatgg tgacaggttc agacggcagc
     5461 atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct gcaacggcga acgacgatcg
     5521 tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca gcaggttatt cattttacgc
     5581 tcggaaacca aaatgccctg caactggtcg cgctcttttt ccagctcact ctgctcaccg
     5641 cgaatcttca tctcttccag tttggcaaga tgacgcagtt tcagttcgag gatcgcttcc
     5701 gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg gtttcggttc atcttcgtta
     5761 cgaatgatct caatcacttc gtcgatgttg agaaacgcca ccagcaaacc ttccaggata
     5821 tgcaggcgct tgaggacttt ctccagacga tagttcagtc ggcggcgcac ggtatcgcga
     5881 cggaacacca gccattcgga gaggatttcc agcaggtttt tcaccgccgg acgaccatcc
     5941 agaccgatca tattgaggtt aatgcgatag ctcttttcca gatcggtggt agcgaagagg
     6001 tggttcatca cctgatccat atccacgcgg ttggaacgcg gcacaatcac cagacgggtc
     6061 gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca tcggcagctt tttgttgcgc
     6121 atttgcgcag caatttgctc cagtacgcgc gcacctgaaa cctgatgcgg caatgcgctg
     6181 ataaccaccg cgccatcttc tttcttccac accgcgcgca tacgcactga accacgtccg
     6241 ttctcgtaga ttttacggat ctcggcgcgc gaagtgataa tttccgcttc agtcggataa
     6301 tccggcccct gcacgatatc cagcagctga tcgagcgtgg ttttcggctg gtcgattaat
     6361 gcgattgccg cctgagctac ttcacgcagg ttatgcggtg gaatatcggt cgccatgccg
     6421 acggcaatac cggtggtgcc gttaagcaaa atgtttggca gacgggcagg cagcattttc
     6481 ggctcctgca aagtgccgtc gaagtttggc acccagtcag ccgtcccctg ccccagctcg
     6541 ctcaatagca gttcggaata tttcgacaac cgggattcgg tgtaacgcat tgccgcgaac
     6601 gatttcggat cgtccggcgc gccccagttc ccctgaccat caaccagcgg ataacggtaa
     6661 gagaacggct gcgccatcag gaccatcgct tcataacagg cactatcgcc gtgcggatgg
     6721 tatttaccca gtacgtcacc gacggtacgg gccgattttt taaatttggc gctggcattc
     6781 aggcccagtt cagacatcgc atacacaatg cggcgctgaa caggtttaag accatcgcca
     6841 ataaacggca acgcacggtc catgatcacg tacatggagt agtttaagta ggcgttttcc
     6901 gtaaattcat gtagcgcaag gcgctctgcc atatcgctca ttaattctga ttcctcaact
     6961 tattcgccag ccttcaaagg ggcaatattg ccggagatac tacattatct gccgcgtcga
     7021 gtcacaggag atcagcgtga atagcgccgc cggacgtgct ccgacggcga taaaggcatc
     7081 aatgtgcctt gatatacaac tctttgctgt agacttgatc ggcatagagg gcagcacaag
     7141 gttgcgccca tgccacacca ctcggcatgg gggtccgctc cgggcgattc gagaagttga
     7201 ggttatgcga gtccagggaa gcctaaagca tcaaagtagt cgactgtaag tatacggttc
     7261 agcccagttt agctgtgtct ccaccagtgc cccgcgcctc ctgccataag tgcggcttgc
     7321 tcatggatac ccccgagacg gcttacggcg caccggataa taaatttcgc gtatacatgg
     7381 tattccttac cggagaaatt ataagcccaa caacaatacg cctgtggcat tgttcgctca
     7441 aacttatcgc gagtgatttg gctcacactc cggtaaagat tccccatcac gctaacggca
     7501 cataaacatc cgtttccagt cgctgttctg tcataccttc ggcaagattg gtgtagtgaa
     7561 acagaatcgg tgctttacgc attttctcgc cgcttgcagg cagccagtgg cgaatgatgc
     7621 cccataccgt gtgtgaaata tcgtccagct cgccaacgtg gcgggccacg gcataacgtc
     7681 caccggtaag ttcaccattg ctcacaccat aacgattatc gggaatcggt tcgctaacgc
     7741 tgccgcagat atcaaagcga aacgctttcg gtgcggtggt ggcagggtcg tcccaggcga
     7801 cgccaaaagt ctggctttgg ttaacaggag aaagccccgt ctctttacgc cacatgataa
     7861 atttcgccac actgtagttg agcaactcag ggctacagcg gtgcggcaac atcgccacag
     7921 ggattgatgg gaaatcgata atgtttacat ccagtgtcag gtttgtcatt ttgcctccct
     7981 gcgaaattat tcccgggaac gcgtcccggg agcggtaaca attacggatt tactttgcgg
     8041 atctgtttga cgtcaatttc aacagaattc cagtctttat cgacttcccc ctgaatctca
     8101 accgtatctt tcggcgtcac cgtcacaccg ttccagcgtt tgtggtcgat atcaacattg
     8161 atagtaccgc tggcatcttt aaacacgtag agatcgtcag agatgcgttc aacgatattg
     8221 ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg cgctttctac agtcgttacg
     8281 ctgccgttcg gcccctggaa tcctccggcc tgactttgcg ttgccgatgg gccagaaaaa
     8341 ccgccctgct ctgctgccat caccggtgca ctgcacaggg ccattactgc gattactgct
     8401 gcgaattttt tcatgtttat tactcccttt aatgtctgtt tccgagcatt taacaagata
     8461 gtccttaaca acttcttaag ggaaaaaaat aaaatttaat gctgtacaga gcgcgttaca
     8521 acacggttta ctggcagcaa atacggttat cgcagggatg aaaaaatgcg aattttactg
     8581 atagaagatg acatgctgat tggcgacggc atcaaaactg gccttagtaa aatgggtttt
     8641 agtgtcgact ggtttacaca aggtcgtcag ggaaaagagg cgctttatag cgcaccttat
     8701 gatgcggtga tcctggattt aaccttacca ggcatggatg gtcgcgatat tttgcgcgaa
     8761 tggcgagaga aaggtcagcg tgagccggta ctgatcctga ccgcgcgtga tgcgttagcg
     8821 gaacgtgtag aagggctgcg tctgggagct gacgattatc tgtgtaaacc ttttgcgttg
     8881 atagaagtcg ccgccaggct ggaggctctg atgcgccgaa ccaacggcca ggccagcaac
     8941 gagctgcgcc acggtaacgt catgctcgac cccggcaaac gtatcgccac gctggctagc
     9001 gaacccttaa cactgaaacc aaaagaattt gccctgctgg aattactgat gcgtaacgct
     9061 ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt atacctggga cgaagaggtc
     9121 accagtaatg ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg tagtgatttt
     9181 attcgtaccg tgcatggtat tggctacaca ttaggtgaga aatgaaattt acccaacgtc
     9241 ttagtctgcg cgtcaggctg acgctaatct ttttaattct ggcctcggtg acctggctgc
     9301 tttccagctt tgtcgcctgg aaacaaacaa cggataacgt cgatgaattg ttcgacaccc
     9361 aactgatgct gtttgccaag cggttaagta cgctcgatct caacgaaatc aacgcggcgg
     9421 atcgcatggc acagacgcca aataaattaa aacacggtca tgttgatgac gatgcgctga
     9481 cctttgccat ctttacccac gacggcagaa tggtccttaa tgatggcgat aacggagaag
     9541 atattcccta tagctatcaa cgggaaggtt ttgctgacgg gcaactggtc ggtgaagacg
     9601 atccttggcg ttttgtctgg atgacctcac ctgatggcaa atatcgcatc gttgttggcc
     9661 aggaatggga ataccgtgaa gacatggcgc tggcgattgt tgccgggcaa ttgatcccgt
     9721 ggctggtcgc actgccgatt atgttaatca tcatgatggt actactgggt cgtgaactcg
     9781 cgccgctgaa caaactggcg ctggcactac gtatgcgtga ccctgactcg gaaaaaccac
     9841 taaacgcgac tggcgtaccc agcgaagtgc gaccactggt tgagtcgcta aatcaactgt
     9901 tcgcccgcac acatgcgatg atggttcgag aacgacgctt tacctccgat gcagctcacg
     9961 aactacgtag cccgttaaca gcgctgaaag tgcaaaccga agttgcgcag ctctctgacg
    10021 atgatccgca ggcacggaaa aaagcactgc tccaattaca ttccgggatc gatcgcgcta
    10081 ctcgtctggt tgatcaactg ctcacgctat cgcggctgga ctcactggat aaccttcagg
    10141 acgtcgcgga gatcccgctt gaagatctcc tgcaatcgtc ggtgatggat atttaccaca
    10201 cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa tgtccagggc atcaaacgca
    10261 ccggacaacc gctattgcta agtttgttgg tgcgaaattt gctggataac gccgtgcgtt
    10321 acagtccaca gggcagcgtg gtggacgtca cgctgaatgc cgataatttc atcgtgaggg
    10381 ataacggccc cggtgtgaca ccagaggcac tggcgcgaat tggcgaacgc ttctatcgcc
    10441 cctccggaca aaccgctacc ggcagtggac ttgggctatc gattgtccag cgaatcgcca
    10501 agctgcatgg catgaatgtt gagtttggga atgcggaaca aggtggattt gaggcgaaag
    10561 taagctggta agggtaagac ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat
    10621 ttcaccgtac cgctctgtga cgtactatag tcggcaaacg tctcaccttg aggttaaaaa
    10681 atgagcaaca ttctgattat caacggcgcg aaaaaattcg cccactccaa tggtcaactg
    10741 aatgacaccc tgaccgaagt cgcggatggc acactgcgcg acctcgggca tgatgttcgc
    10801 atcgttcgcg ccgacagcga ctacgatgtc aaagcggaag tacaaaactt tctctgggct
    10861 gatgtggtga tctggcagat gccaggctgg tggatgggcg cgccgtggac agtgaaaaaa
    10921 tacattgatg atgtattcac cgaaggtcac gggacgctgt atgccagcga tggccgtacc
    10981 cgcaaagatc cgtcaaaaaa atacggttcc ggcggcctgg tacagggcaa aaaatatatg
    11041 ctttctctga cctggaacgc accaatggaa gccttcaccg aaaaagatca attcttccac
    11101 ggcgttggcg tcgacggtgt gtatctgccg ttccataaag caaaccaatt ccttggcatg
    11161 gaaccgctgc cgacatttat cgctaatgac gtgataaaaa tgcctgatgt tccccgctat
    11221 actgaagaat atcgcaagca tcttgtggaa atttttggtt aactagagct caggctttag
    11281 aaggagttaa ccatgcttac cgtaatcgca gaaatccgta ctcgtcctgg tcaacatcac
    11341 cgtcaggcgg tattggatca gtttgctaaa atcgttccaa ccgtactgaa agaagaaggt
    11401 tgccacggct atgcgccaat ggtggattgc gctgctggcg tgagtttcca gtctatggca
    11461 ccggattcta tcgtgatgat tgagcagtgg gaaagcatcg cgcaccttga agcgcatctg
    11521 caaaccccgc acatgaaggc gtacagcgaa gccgtaaaag gtgacgtgct ggagatgaat
    11581 attcgtattc tgcagccagg gatttaatcc tgccttgttt gcccggccat cctgaccggg
    11641 caatgttctt tcctttaaac ctcaatctcc gccatgtcgc ctttctcttg caaccagttg
    11701 cggcgatctt ccgagcgttt cttcgccagt agcatgtcca tcatcgcgtc agtacgctga
    11761 tcgtcttcat catcgatagt caactgcacc agacggcgag tgttcggatc aagcgtggtt
    11821 tcgcgcaatt gcatcgggtt catttccccc agacctttaa aacgctggac gttcggcttg
    11881 cctttcttgc gttttaattg ctcaagtacg ccctctttct cttcttccgt cagcgcgtaa
    11941 taaacctctt tcccgagatc aatacggtag agcggtggca gtgcgacgta aacgtgaccg
    12001 tgtttcacca acgcgcggaa gtgttttaca aacaaagcgc agagcagcgt ggcaatgtgc
    12061 agaccatcgg agtcagcatc cgccaggata cagatcttgc cgtaacgaag ctggctcaaa
    12121 tcgtcgctgt caggatcgat accgatcgct accgaaatat cgtgcacttc ctgcgaagcc
    12181 agcacttcgt cggaagagac ttcccaggtg ttaaggatct tacctttcag tggcatgatc
    12241 gcctgatatt cgcgatcgcg cgcctgcttg gcagatccgc ctgcggagtc accttccaca
    12301 agaaacagtt cggtgcggtt aaggtcctgc gcggtacagt cagccagttt gccaggcagc
    12361 gccggaccgc tggtcagctt tttacgcacc acttttttag ccgcacgcat acgacgctgg
    12421 gcgctggaaa tcgccatctc tgccagcaac tccgccgcct gaacgttctg gttcagccac
    12481 aggataaagg catctttcac cacgccagaa acgaatgccg cgcactgacg cgaagagaga
    12541 cgctctttgg tctgcccggc aaactgcgga tcctgcattt ttactgacag cacataggcg
    12601 cagcgatccc agatatcttc cgccgacagc tttacaccgc gcggcagaat gttgcggtat
    12661 tcacagaact cacgcatcgc gtccagcagg ccctgacgca gaccattaac atgggtaccg
    12721 ccctgcatcg ttgggatcag gttgacgtag ctttcggtca gcagttcacc gccttccggc
    12781 agccacagta gcgcccagtc caccgcttca gtatcgccag cgaaattacc gataaacggt
    12841 ttttctggca gcgtcggtaa accgtttacc gcttccgcca ggtaatcatt cagaccgtcc
    12901 tgatagcacc agcgctgttc ggtgttgttg atctcatctt taaaggtaat ctcaacgccg
    12961 gggcacagta ctgctttggc tttcagcaca tgcgtcaggc gtgaaacaga aaaacgcggg
    13021 ctatcgaaga aggtttcatc cggccagaaa tggacgctgg ttccggtatt gcgtttaccg
    13081 caagtgccga caacctgtaa atcctgcacc ttttcgccat tttcaaaggc gatgttataa
    13141 atctgaccat cgcggcgcac gttaacttct acgcgcttcg acagggcgtt aaccaccgaa
    13201 atccccacgc catgcaggcc gccagagaac tggtaatttt tgttagagaa tttaccgcct
    13261 gcatgcagac ggcaaagaat cagttcaacc gccggtacac cctcttccgg gtgaatatcc
    13321 accggcatcc cgcgcccatc gtcaataact tctaacgact ggtcagcatg taaaataacg
    13381 tccacgcgtt ttgcgtgacc cgccagtgct tcatccacac tgttatcaat gacttcttgc
    13441 cccaaatggt tagggcgagt ggtatcggta tacatccccg gacggcggcg aaccggctca
    13501 agcccggtga gtacctcaat ggcatcagcg ttataagttt gcgtcatggt ttaagttagt
    13561 aattcgagtt gatcgtcaga gatggtgcag accaagaaaa tcgacgatcg ggttgaaata
    13621 atcttcgaag cccgtgaatg cgtggttgcc gccttctatg acagtctggc ggcaggaagc
    13681 gtagtacgcc accgcctggc ggtaatccag cacttcatct cccgtctgtt gcagcagcca
    13741 gatcaaatcc ggcgcttcca gcgggtcaat ctgcatgact ttaagatcgt aaatatggcg
    13801 tgactctagc acatattgct gcccggtgta ggggttctcg ttctgaccga gatagtccgt
    13861 cagcagttca aacgggcgca ccgccgggtt taccaccact gcgggcagca taaaacattg
    13921 tgacaaccag gtggcgtaat atccccccag tgacgaaccg acaataccca gcgaatcgcc
    13981 gccatgttcc aggacaatgg attccagcag ctctgccgcg tcggaaggat acggcggcaa
    14041 ctgcggaatg atcatctcaa cgtcagggtg atgttccgcc agccagtttt ttaacaagct
    14101 cgcttttgca gagcgcggcg agctgttgaa accgtgtaaa taaagaagcg tagacatcag
    14161 tagccttctg aagcggtatc aggttggaaa cgtgtgtccg ccaggcgatg cacctcggtg
    14221 gtcagcgtgc catcagcatg taactcgaga gtacgccagc cgggcgcgat ggtatccagc
    14281 gtaaagttgg aacagtgcgg cttaaactgc acacaggtcg acggcgttgc cagcaggcgg
    14341 cgaccgttcc agtcgagatc cagctcctga tgaatatgac cgcacagcaa gtatttgacg
    14401 tgcggaaact tcgccagcac ggtatccagt tcgcccgcgt tacgcagact gtgttgatcg
    14461 agccaactac aacccgcagg tagcggatga tgatgcagca gcagcaacgt atggcgttct
    14521 ggcgcatcgg ccagtttacg ttccagccac tcaagctgaa actcgctcag ctcaccgtgc
    14581 ggcacgccaa acacctggct atccagcaac aggatttgcc attgctcacc aataaacacg
    14641 cgcttcgccg gggagatacc cgcatcctgt aacgcgctgt acatcgcggg ctggaaatcg
    14701 tggttgcccg gcagccagac gcagggcgca cgaaaacttg cgatgccttc agcgaaatgc
    14761 tgataggccg cagaggattg atcctgcgct aaatcacctg tcgcgacaat caggtcgaat
    14821 tcgtgctgat gtggacgaat cgcctccagc accgcctggt agctttccca ggtgtttacc
    14881 cctaacaggg cttcgtgctt ttgtgcaaac aggtgagtat cggtaatttg taaaatcctg
    14941 actctggcct caccagccag aggaagggtt aacaggcttt ccaaatggtg tccttaggtt
    15001 tcacgacgct agtaaaccgg aatcgccatc gctccatgtg ctaaacagta tcgcaaccag
    15061 tccgctaaaa actggttaat ttgatgcttt tcgtcgcgtt gatgcaactt tttattagga
    15121 taatcatacc gcgctttgaa gcgaaaaatc tgctggcttg aacacacttc agccaccatc
    15181 gcgtcatgat acagacgcac cgtcattgac ggaaggctcc agtaactgat cgcgggcgca
    15241 gtctgttcta ttgtcaccag ggtagtgtat cgggtcgatt ccacaatcgt cagccgatat
    15301 tgtgcgtttg ccacctgata gcttacagtt tcgccgggtg cgtcattgcg cggtaacaaa
    15361 cggcgcaatt gtgaaaagtt catctcgcac aggcgcatca tttcaggaaa gtcaggtgtg
    15421 taacgcttca tttatgccca ctcatttttt aacgcttggt ggtgcagctg cagccattgc
    15481 aaagcgatga ccgacgctgc gttgtcgatt ttcccctctt ctacccactg gtatgcctgt
    15541 tcccggctta ccacatgaac gcgaatatct tcgttttcat cagccagacc atgaataccg
    15601 cttgcggtcg tggcgtccac ttcgcccacc ataattgacg aacgctcact ggtgcccccc
    15661 gggcttgcca ggaaacttaa caccggtttg gtccgtttga ctatcagccc cgcctcttca
    15721 atcgcttcgc gacgggcaac atcttccaca ctttcaccct cttcaatcat cccggcaacc
    15781 atctccagta gccagggggt ttcgctagtg tcgtacgcgg caattcgaat ctgctcaatc
    15841 agcacaactt catcacgcac tgggtcaaag ggtagcaaga ctgcggcgtg accgcgctca
    15901 aaaatttccc gccgcacctc atgactcatt tgcccgttga atagactatg acgaaatcta
    15961 taaagatcta atgaaaaaaa gccgcgataa agtgtttctc gtgcaataat ttctacatcg
    16021 tttttgccaa atgtaacggg caggttgtct ggcttaagca ttgttaatgt cctggcacta
    16081 atagtgaatt aaatgtgaat ttcagcgacg tttgactgcc gtttgagcag tcatgtgtta
    16141 aattgaggca cattaacgcc ctatggcacg taacgccaac cttttgcggt agcggcttct
    16201 gctagaatcc gcaataattt tacagtttga tcgcgctaaa tactgcttca ccacaaggaa
    16261 tgcaaatgaa gaaattgctc cccattctta tcggcctgag cctttctggg ttcagttcgt
    16321 tgagccaggc cgagaacctg atgcaagttt atcagcaagc acgccttagt aacccggaat
    16381 tgcgtaagtc tgccgccgat cgtgatgctg cctttgaaaa aattaatgaa gcgcgcagtc
    16441 cattactgcc acagctaggt ttaggtgcag attacaccta tagcaacggc taccgcgacg
    16501 cgaacggcat caactcgaac gcgaccagtg cgtccctgca gttaactcaa tccatttttg
    16561 atatgtcgaa atggcgtgcg ttaacgctgc aggaaaaagc agcagggatt caggacgtca
    16621 catatcagac cgatcagcaa accttgatcc tcaacaccgc gaccgcttat ttcaacgtgt
    16681 tgaatgctat tgacgttctt tcctatacac aggcacaaaa agaagcgatc taccgtcaat
    16741 tagatcaaac cacccaacgt tttaacgtgg gcctggtagc gatcaccgac gtgcagaacg
    16801 cccgcgcgca gtacgatacc gtgctggcga acgaagtgac cgcacgtaat aaccttgata
    16861 acgcggtaga gcagctgcgc cagatcaccg gtaactacta tccggaactg gcggcgctga
    16921 atgtcgaaaa ctttaaaacc gacaaaccac agccggttaa cgcgctgctg aaagaagccg
    16981 aaaaacgcaa cctgtcgctg ttacaggcac gcttgagcca ggacctggcg cgcgagcaaa
    17041 ttcgccaggc gcaggatggt cacttaccga cgctggattt aacggcttct accgggattt
    17101 ctgacacctc ttatagcggt tcgaaaactc gtggtgccgc tggtacccag tatgacgaca
    17161 gcaatatggg ccagaacaaa gtgggcctga gcttctcgct gccgatttat cagggcggaa
    17221 tggttaactc gcaggtgaaa caggcccagt acaactttgt tggtgccagc gagcaactgg
    17281 aaagcgcgca tcgtagcatc gtgcaaaccg tacgttcctc cttcaacaac attaatgcat
    17341 ctatcagtag cattaacgcc tacaaacaag ccgtagtttc cgctcaaagc tcattagacg
    17401 cgatggaagc gggctactcg gtcggtacgc gtaccattgt tgatgtgttg gatgcaacca
    17461 ccacgctgta caacgctaag caagagctgg caaatgcgcg ttataactac ctgattaatc
    17521 agctgaatat taagtcagcc ctgggtacgt tgaacgagca ggatctgctg gcactgaaca
    17581 atgcgctgag caaaccggtt tccactaatc cggaaaacgt tgccccgcaa acgccggaac
    17641 agaatgctat tgctgatggt tatgcgcctg atagcccggc acccgtcgtt cagcaaacat
    17701 ccgcacgcac taccaccagt aacggtcata accctttccg taactgatga cgacgacggg
    17761 gcttcggccc cgtctgaacg taagacaacg taaagatacg ggttatctgc cgcattcttc
    17821 ccccttctcg cttcaatttc gaccagccat cctctattct gatgggtatt taccactggt
    17881 cccggaagac aaaaatgaaa cggacaaaat ccatacgcca cgcatcgttc cgcaaaaact
    17941 ggagcgcacg ccatctgaca ccagtcgctc tcgcggttgc cactgttttt atgctggcta
    18001 gctgtgaaaa gagtgatgaa acagtgtctc tctatcaaaa tgctgacgac tgttcagctg
    18061 caaacccagg caaaagcgcc gaatgtacca ccgcgtacaa caatgcgctg aaagaagccg
    18121 aacgtactgc gccgaaatac gccacccgtg aagactgtgt tgctgaattt ggtgaaggtc
    18181 agtgccagca ggcaccagcc caggctggca tggcaccaga aaaccaggcg caggcccagc
    18241 aatccagcgg gagtttctgg atgccgctga tggccggtta catgatgggg cgtctgatgg
    18301 gcggcggcgc gggatttgca cagcagccgc tgttctcctc gaaaaaccca gccagcccgg
    18361 cttacggtaa atataccgac gcgacgggta aaaactatgg cgcagcccag ccaggccgca
    18421 ccatgaccgt accgaagaca gccatggcac caaaaccggc gaccactact accgttaccc
    18481 gtggcggttt tggtgaatct gttgccaaac aaagcactat gcagcgtagc gcaaccggca
    18541 cctcttctcg ttcaatgggt ggctgatacc gatggaaaga gtcagtatta ccgagcgccc
    18601 ggactggcgt gaaaaagccc acgaatacgg tttcaatttt cacaccatgt acggcgagcc
    18661 gtactggtgt gaagatgctt actacaagtt gcccctcgcc caggttgaaa agctggaaga
    18721 agtcaccgcc gaactgcacc agatgtgcct gaaagtggtg gaaaaagtga tcgccagcga
    18781 tgagctgatg accaaattcc gcattccaaa acacacctgg agttttgtgc gtcagtcatg
    18841 gctgactcac cagccatcgc tttattcgcg tcttgatctg gcatgggatg gcactggcga
    18901 acctaaactg ctggaaaata acgccgatac accaacgtca ctatacgagg cggcgttctt
    18961 tcagtggatc tggctggaag atcagcttaa cgccggtaac ttgccggagg gcagcgacca
    19021 gtttaacagt ctgcaagaaa agctaatcga tcgctttgtc gagttgcgtg aacagtatgg
    19081 cttccagttg ctgcatctca cctgctgtcg cgacacggtg gaagatcgcg gaaccattca
    19141 gtatttgcag gactgcgcga cggaagctga aatcgctacc gagttcctct acattgacga
    19201 tatcgggtta ggtgaaaaag gtcagttcac ggatttacag gatcaggtaa tttccaacct
    19261 gttcaagctg tatccatggg aatttatgtt gcgtgagatg ttctccacca agctggagga
    19321 tgcaggcgta cgctggctgg aaccggcgtg gaagagcatt atctccaaca aagcgcttct
    19381 accgctactg tgggagatgt tcccgaatca cccgaacctg ctgcccgctt attttgcgga
    19441 agatgatcat ccgcaaatgg aaaaatatgt ggttaaaccg atcttctccc gtgaaggcgc
    19501 aaacgtgtcg atcattgaga acggcaaaac cattgaagca gcggaaggtc cgtatggcga
    19561 agaagggatg attgttcagc aattccatcc gttaccgaaa ttcggcgaca gctatatgct
    19621 gattggtagc tggctggtga acgatcaacc cgccggaatt ggcattcgtg aagaccgtgc
    19681 attgatcacc caggatatgt ctcggtttta tccacatatt tttgttgagt aatcaatgat
    19741 gccggatggc attcgccatc cggtaattgt taacctatct gcaccgacag catactcagg
    19801 ctgcccattt ctataccatc gaccggaatg gtaattggct cctgcccatc ccacgcacct
    19861 aagacataca acaacggcag atagtgctcc ggcgttgggt tcgataacgc gccaccttca
    19921 tggtcgaggt aattcaccag aggatgctgc tctaccgccc cttgccacgt cagattcgct
    19981 ttcacatact cattaaacga catcgcccaa ggatatggtg aactatcacc gtgccacttc
    20041 actgtacgca ggttatgcac cacgttgcca ctggcgacca acattattcc ttcatctcgc
    20101 agcgctgcca gtttgcgccc catttcgaaa tgccaggcgg caggtttgct actgtcgata
    20161 ctcaactgca ccatcgggat atcggcgtca ggatacatct taatcagcac gccccaggaa
    20221 ccgtggtcaa agcccctggc ttctttatcc agcgtcaccg ggaccggcgc taacagctca
    20281 accagatgct gtgccagcac aggcgaaccc ggagcaggat aatgcgtatc gtacagcgcc
    20341 tgcgggaagc caccaaagtc atgaatcgtg ggcggcgtct ccatcgcggt cactcctgtt
    20401 ccacgggtaa accagtgagc cgaaaccacc acaatcgctt gcgggcgtgg caatgtcatc
    20461 cccaacttct gccagctgcg ggtatacaaa ttatcttcca gcacgttcat cggactaccg
    20521 tgacctaaaa acaatgctgg catacgtgtt gaagacatga tgatatcctt aactaagggt
    20581 gtcattttga tatcctcaca atacgcttgt tcggcggagt aagaacccgg ataacaatga
    20641 tgatgatcat cagttatttt gacgatctgc ctgaaggtga agatttataa ggagttgtcg
    20701 atgtcagtac ctctcattct gaccatactg gcgggggcag ccacgtttat tggcgcgttt
    20761 ctcggcgttc tcggacaaaa accctcgaac cgcttactgg cgttttcgct aggttttgcg
    20821 gcggggatca tgttgctcat ctcattaatg gaaatgcttc ctgccgcact agcagctgaa
    20881 ggaatatcgc ctgtgttggg ttatggaatg tttatcttcg gtctgcttgg ctactttggc
    20941 ctggaccgca tgttgccgca tgcgcatccg caggatttaa tgcaaaaatc ggtgcagccg
    21001 ttgccaaaat cgatcaagcg cacagccatt ctgctcactc tcggcatcag tctgcataac
    21061 ttcccggaag ggattgccac ctttgtcacg gcgagcagca acctggagct gggatttggc
    21121 atcgcactgg ccgtcgcgtt gcacaatatc cctgaaggtc tggcagtggc aggcccggtt
    21181 tatgcggcaa cggggtctaa acgcaccgcg attctgtggg cggggatttc tggactagca
    21241 gaaattcttg gtggtgtgct ggcgtggtta atcctcggta gcatgatttc cccggtggtc
    21301 atggcggcgg ttgcaggaat tatggtggcg ctctcggttg atgaattaat gccgctcgcc
    21361 aaagagattg accctaataa taaccccagc tatggcgtac tatgtggaat gtcagtgatg
    21421 ggattcagtt tagtgctgct acaaacggcg ggaattggtt aaaaacaaca tcagaagttg
    21481 tccagcacat ggacaacttc tgccagcttc acgaaagcct tattctacct tttcggctgt
    21541 ctcttctctc gtactgttta agtatttgtt ccgcgcttca agcagtgcgt ctctgtcctg
    21601 gcgaaacgcc cgacaataac gtttcaaatg caggaaataa cccaccacaa ccagagcaat
    21661 caatacaatc cagtaccagg cgataaacat cctttaccct taatatttaa caatactaaa
    21721 catttagcgc ataaatttca catatccttt ttcggatata ttcatatggt cgcataataa
    21781 cggaacaatt atatgatttt gatcataatg aaaaagaatg cctgatatta atgaaatttt
    21841 ctcatagaaa gagaagcgga attaacatct tgcatagcag ggcaaaataa aaaccgaagc
    21901 cagctacaac ttcggtttct tgattaaggc agtaattaag cagcggtttt cagctggctt
    21961 tacgctcatg tgcctgacgg tatgccacca ggtcttcaat agtcacgagc gccatattgt
    22021 gtttattggc aaactcaatg cactctggtg cacgcgccat cgtgccatcg tcattagtca
    22081 gctcacacag cacaccagcc ggtttaaagc ctgccagcgt catcaaatca atagtcgctt
    22141 cagtatgacc gccacgcgtc agtacaccac ctgcctgcgc gcgaagtggg aaaacgtggc
    22201 ccgggcgatt cagatcagaa ggttttgcgc catcggcaat cgctgcgcga acggtcgtaa
    22261 tacgatcagc ggcagaaaca ccggtagtca caccttcagc cgcttcaatg gtcacggtaa
    22321 aaccggtgcc ataggcgctg gtgttatttt ctaccatcat tggcagatca agttgtttac
    22381 ggcgatcttc agtgatgcac aggcaaacaa taccgctacc gtggcgaatg gtcagcgcca
    22441 tctgctcaac ggtcatggtt tctgccggga agatcatatc gccttcgttt tcacggtctt
    22501 catcatcaag caccattaca ccgcgtcctt cacgcagcgc agccagtgca ttttcaacac
    22561 gttcgaaagg cgtaccaaaa gaggaaagta gcgtctgatt catggtaaaa aaaactcact
    22621 aaaattatgg ttaccagaat cagggcagtc ttaggagtgg cggcatatag ccaaaataac
    22681 gtgagcgggt ccatgcccga cagaatcgtt actctctccc atccggactc taaccgtcgg
    22741 ccccggaatt acaccggatc tgctgtcctt tgagttcgca cccaaagcgc tcgcgggctt
    22801 tcaactgagt tgatttaccg ccggtgggga atttcgcccc gccctgagaa taagcggatt
    22861 cactataacg ctaatgatta gcggcagcaa cgcatagctt cacataattc tggtttatga
    22921 cttaccctta tcgcactaca atggtactca acacctatca gtacagggaa actacaatga
    22981 ttgacccgaa aaaaattgag caaatcgctc gccaagttca cgaatcaatg cctaaaggaa
    23041 tcagggagtt cggggaagat gtggagaaaa aaatccgcca aaccctacaa gctcagctga
    23101 cgcgcctcga ccttgtaagc cgtgaagagt tcgacgtcca aacgcaagtc ctgttacgga
    23161 ctcgtgagaa actggcgctg cttgaacaaa gaatgagcga actggaaaat cgcagtactg
    23221 agattaaaaa gcagccagat ccagagactc tccccccaac gctgtaaaca tttgtcttta
    23281 atgcactgta aattatgaat taatcttcta atttacagtg ctttctatta ccttatcgct
    23341 taattccatt cagaatatca ccacaaaaat gaatgagtgc aattgaaaat ataattgtta
    23401 tgtatttttt taagttttac ttaatccata tttagataaa ctaatttcat aaaacccgaa
    23461 aagtaaaata taatatttga atgattagca atccttaaat aacagttact aatcattcat
    23521 tttctactta tatttatata aaggaatatt atgtctgctt ttaaaaagtc acttcttgtt
    23581 gctggcgtag caatgattct ttccaataac gtatttgctg acgaaggtca cggcattgtt
    23641 aaatttaaag gtgaagttat ctctgctcca tgttcaatta aaccaggtga cgaagactta
    23701 actgtaaatc tgggtgaagt agcagatacc gttctgaaaa gcgaccagaa atctttggct
    23761 gagcctttca ctattcacct gcaagactgc atgttgagcc agggtggaac cacttattca
    23821 aaagcgaagg ttactttcac cacagcgaat actatgacgg gtcagactga cctgctgaaa
    23881 aacaccaaag aaactgaaat cggtggtgcc acaggcgttg gcgtacgtat tctgaatagc
    23941 cagagcggcg aagtgacttt aggtactccg gtagtaatta cattcaacaa tactaacagc
    24001 tatcaggaac tgaacttcaa agttcgtatg gaatccccgg gcaaagatgc taccccgggc
    24061 aacgtttacg ctcaggctga ctataagatt gcttacgagt aatcacatta ggccgggttc
    24121 gcccggccac atcattaacg gattaatgat aagtggaaca gatgtataaa aaattaaaat
    24181 taaccacaat aagcgaattg attaaaaata tttattgttc attatccgtt ctcatcattg
    24241 gttgtgcgtc agcttatgcc gttgaattca acaaagattt aatcgaagcc gaagatcgtg
    24301 aaaacgttaa cctttcccaa tttgaaactg atggccaatt acccgtaggt aaatactcat
    24361 taaacgctat tattaataat aaaaggacgc caatccatct ggacctgcaa tgggtattaa
    24421 ttgataacca aactgcagtt tgtctgactc cagagcaatt aacattatta ggctttactg
    24481 atgagattat tgaagaggct cagcaaaacc tgatcgatgg ttgttaccct atcgaaaaag
    24541 aaaaacaaat tacaacttat ctcgataaag ggaaaatgca attatccata tctgcacctc
    24601 aggcatggtt aaaatacaaa gatgcaaact ggacgcctcc tgaactttgg gatcatggta
    24661 ttgctggggc atttcttgac tacaatttat atgcctctca ttatgcacca catcagggcg
    24721 ataattcaca aaatataagt tcctatgggc aggctggggt taatcttggg gcctggcgcc
    24781 tgcgtactga ttaccagtac gatcagtcat ttaacaatgg caaaagccag gcgaacaacc
    24841 tggattttcc gcgtatttat ttgtttcgcc caatcccagc aattaatgca aaactaacta
    24901 taggtcaata cgatactgaa tcctctattt tcgactcttt ccatttttct ggcgtttcgt
    24961 tgaaaagcga tgagaatatg ttgccgcctg atctacgtgg ttacgcaccg caaatcacgg
    25021 gtgttgcaca aacgaatgca aaggtcactg tctcacagaa caaccgtatt atttatcaag
    25081 aaaatgttcc tcctggccca tttgctatta ctaatttatt caatacatta caggggcaac
    25141 tggacgtcaa ggttgaagaa gaggacgggc aagtcacgca atggcaagtt gcatctaata
    25201 gtattcctta tctgacgcgt aaagggcaga ttcgctacac cactgctatg ggtaaaccga
    25261 ccagcgttgg tggtgattcc ttacaacaac ccttcttctg gactggtgaa ttctcatggg
    25321 gttggctgaa caatgtatcc ctgcatggtg gttcagtttt aacaaaccgt gattatcaat
    25381 ctctggctgc cggcgttggc tttaacctta actcattggg ttcattatct tttgatgtca
    25441 cacgatctga tactcagttg cataatcagg ataaagaaac gggttatagc taccgcgcta
    25501 actattcaaa acgttttgaa tctaccggta gccagctcac tttcgctggt taccgtttct
    25561 ctgataaaaa ctttgtgaca atgaatgaat atatcaatga cactaaccat tacacgaatt
//
LOCUS       DAJTNN010000041        25512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTNN010000041 DAJTNN010000000
VERSION     DAJTNN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.041"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            353..1711
                     /locus_tag="OO150_001509"
     CDS             353..1711
                     /locus_tag="OO150_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5515702.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1950..3335)
                     /locus_tag="OO150_001510"
     CDS             complement(1950..3335)
                     /locus_tag="OO150_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5515703.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OO150_001511"
     CDS             complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OO150_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5515704.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3729..4118)
                     /locus_tag="OO150_001512"
     CDS             complement(3729..4118)
                     /locus_tag="OO150_001512"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5515705.1"
                     /translation="MSDMQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALE
                     NGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEV
                     TFRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4464..4898)
                     /locus_tag="OO150_001513"
     CDS             complement(4464..4898)
                     /locus_tag="OO150_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5515706.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4886..5287)
                     /locus_tag="OO150_001514"
     CDS             complement(4886..5287)
                     /locus_tag="OO150_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515707.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5547..6116)
                     /locus_tag="OO150_001515"
     CDS             complement(5547..6116)
                     /locus_tag="OO150_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5515708.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6861..7004)
                     /locus_tag="OO150_001516"
                     /pseudo
     CDS             complement(6861..7004)
                     /locus_tag="OO150_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(7338..7604)
                     /locus_tag="OO150_001517"
     CDS             complement(7338..7604)
                     /locus_tag="OO150_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515709.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7745..7951
                     /locus_tag="OO150_001518"
     CDS             7745..7951
                     /locus_tag="OO150_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5515710.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            8046..8648
                     /locus_tag="OO150_001519"
     CDS             8046..8648
                     /locus_tag="OO150_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515711.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSPEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            9848..10765
                     /locus_tag="OO150_001520"
     CDS             9848..10765
                     /locus_tag="OO150_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515712.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            10850..11722
                     /locus_tag="OO150_001521"
     CDS             10850..11722
                     /locus_tag="OO150_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5515713.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            12094..14940
                     /gene="ag43"
                     /locus_tag="OO150_001522"
     CDS             12094..14940
                     /gene="ag43"
                     /locus_tag="OO150_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5515714.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            15011..15169
                     /locus_tag="OO150_001523"
     CDS             15011..15169
                     /locus_tag="OO150_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5515715.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            15324..16142
                     /locus_tag="OO150_001524"
     CDS             15324..16142
                     /locus_tag="OO150_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5515716.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            16484..16957
                     /locus_tag="OO150_001525"
     CDS             16484..16957
                     /locus_tag="OO150_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5515717.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            16973..17449
                     /locus_tag="OO150_001526"
     CDS             16973..17449
                     /locus_tag="OO150_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5515718.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            17512..17733
                     /locus_tag="OO150_001527"
     CDS             17512..17733
                     /locus_tag="OO150_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5515719.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            17896..18270
                     /locus_tag="OO150_001528"
     CDS             17896..18270
                     /locus_tag="OO150_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5515720.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            18317..18691
                     /locus_tag="OO150_001529"
     CDS             18317..18691
                     /locus_tag="OO150_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5515721.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            18688..19179
                     /locus_tag="OO150_001530"
     CDS             18688..19179
                     /locus_tag="OO150_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515722.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            19191..19388
                     /locus_tag="OO150_001531"
     CDS             19191..19388
                     /locus_tag="OO150_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515723.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            19473..20033
                     /locus_tag="OO150_001532"
                     /pseudo
     CDS             19473..20033
                     /locus_tag="OO150_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            20105..21109
                     /locus_tag="OO150_001533"
     CDS             20105..21109
                     /locus_tag="OO150_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5515724.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            21391..21672
                     /locus_tag="OO150_001534"
                     /pseudo
     CDS             21391..21672
                     /locus_tag="OO150_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(21822..21909)
                     /locus_tag="OO150_001535"
     tRNA            complement(21822..21909)
                     /locus_tag="OO150_001535"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21873..21875),aa:Ser,seq:gga)
     gene            22143..23081
                     /gene="ghrA"
                     /locus_tag="OO150_001536"
     CDS             22143..23081
                     /gene="ghrA"
                     /locus_tag="OO150_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR5515725.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            23136..23873
                     /gene="ycdX"
                     /locus_tag="OO150_001537"
     CDS             23136..23873
                     /gene="ycdX"
                     /locus_tag="OO150_001537"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5515726.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            23897..24451
                     /gene="ycdY"
                     /locus_tag="OO150_001538"
     CDS             23897..24451
                     /gene="ycdY"
                     /locus_tag="OO150_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5515727.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWMEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            24553..25044
                     /locus_tag="OO150_001539"
     CDS             24553..25044
                     /locus_tag="OO150_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5515728.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(25108..>25512)
                     /locus_tag="OO150_001540"
     CDS             complement(25108..>25512)
                     /locus_tag="OO150_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCR5515729.1"
                     /translation="GYESNVKSGGVGARYFGIGADTQYQLDQIAVNLRVVNVSTGEIL
                     SSVNTSKTILSYEVQAGVFRFIDYQRLLEGEVGYTSNEPVMLCLMSAIETGVIFLIND
                     GIDRGLWDLQNKAERQNDILVKYRHMSVPPES"
BASE COUNT         6164 a         6628 c         6633 g         6087 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa aagtaagcgt
     1921 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     1981 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     2041 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgct
     2101 ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc acgcaaccag
     2161 ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag ccgctgctct
     2221 gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg ctccagggct
     2281 tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttcg gcgggcatga
     2341 gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa cccggcgtac
     2401 gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg gatgcttttt
     2461 ctgtccgggc tgtaagcgaa ccacactgca ggtgccaacg ctgacccggc attgcggtca
     2521 tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg cagcagtacc
     2581 tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc atgaagcgcc
     2641 tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag cagtgaacgg
     2701 ctcagctcca caccttgccg gccgtatatt tctgactggc aatacagcgg ggtgtgctct
     2761 gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc ccgctcgatg
     2821 ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc atgtttttcc
     2881 cgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc
     2941 tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc aggcaacagt
     3001 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
     3061 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tttctgaagc
     3121 cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga acgactgccg
     3181 aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc gcgctcccgg
     3241 ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag gcgctgtttc
     3301 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
     3361 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttggg
     3421 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
     3481 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
     3541 cagtccgtcg ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
     3601 gaaaatatgg ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc
     3661 gttgaagcta tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
     3721 taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg
     3781 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
     3841 gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac aggaagtagc
     3901 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
     3961 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
     4021 tcacaggagg cagcaacgag cagctgttta aattcgggag gataattagg gcagcctttt
     4081 cgcctgccgg gagtcacatt tttctgcata tctgacactt tggttcccac tacttatttg
     4141 gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc tgtacgctta
     4201 ccactatttt atccctgttg aatttggtta agccggtgca gacgcgccgg gaaacgcaca
     4261 cctgaaccgg cacaataaaa tacaatacaa ccccctgtta ctggagatac gaatggaatg
     4321 acgaggcagc ctgtcagtca cagggggctg ttggcataaa atcaacggaa aggccaccaa
     4381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa aggcatcacc
     4441 gcaaactgct ccggaggcaa ccattacagc ctctgtaacg gattatcagc cctggcatct
     4501 tccgacggca cctcctgccg cccccacgcc ctgctgtata ccccggtacc gcagcgtggg
     4561 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     4621 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     4681 gggtcaagcc aggtacatcg cagcaggatt ttttcagcga cagtcatctg gtcatgttca
     4741 aaccacggtg gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
     4801 cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt tcgccgtaac aaaatggcct
     4861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
     4921 gaatactgaa tcctccggtc tggcagtgtg tgcagtactg ctccccataa taatcgtggt
     4981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
     5041 gctgcagacg tttaatcaac tgtatttctc tgcgctccgg cctgacataa gggcactgtt
     5101 gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca gggaagttca gtttcgtatt
     5161 gcggatggta tctgagtgca ctgccacaca agtggcaggt gtagttagca taaggtgcgg
     5221 cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc aagagcgata aaggattttg
     5281 cgtacatagt catatacctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
     5341 ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga gtttgggtaa
     5401 agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggaacact gacgtaaaaa
     5461 acagtcagtg ggcgagtgga tacatcgttg ccgccagaca acaacccata cttttggttt
     5521 gccggatatt ggcgcgccag tcgtgctcaa ctccggctac agccaaaatt gcgctcaccg
     5581 gtgcagtgag ctttggtatg ctccttcgcc agatagtcag cacgttccag cacttgctga
     5641 aagccggtgt catcattacg cgccagccac accgccggcc aggccggaaa atgcaccagc
     5701 gtggcatggc acccgacatc cactcccagg gcactgcacc acgcctgttt aatcatcccg
     5761 gccagagact ccggagcgcg gtaatcaccg gcacgacacc aggtatcccg gttgaccagc
     5821 agcatcaggt gatagtgttt tttacctttg cactccccaa actcccgggc ccagacgtaa
     5881 tgcagggttg tgggatgcac gcgtttacct tcccgatgct tacgtttctg gtaagcgtcg
     5941 attcgggctt tcagggcatt gatgaagcgg gatatcacag ctgcgtccgt tgctgccggt
     6001 acatccggga gacgcaggtc aacccgcaat gccgtcaggc ggggatgaac attcagtgcg
     6061 tgccgcaccg tctcacgaat acgttgctgc cagaaggggt tgtatttgta ggtcatggtt
     6121 aaatctccgt atggttcata cggaatagcc acgtcgtaaa aaatacgcag agtccctgaa
     6181 gtgacctccg gcaggacact gtctcaggca ctctgtgata acccgaacgg tcgtctccgg
     6241 actgacggtt gaattttcag cgtcgttttt taatgtaaaa aaccggttac gggtaaggct
     6301 gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag ggactgtgga aacgcgatta
     6361 ctcataatac agtgtgttgc ccgtattgtg gttctgtcga ccccttcggc tactaccgga
     6421 atacagaccg tatcgttacc atactgctgg cgctgattat tgtggtcctg ctgactactg
     6481 tcagcgtcag tgtgtgtata ctgtgtagct ggtgagataa cggttcatgt atcccctgag
     6541 ctgacagtgt accgtgtata ttaagcgtaa ccgacaggtt ctccttcata ccatattaaa
     6601 ttttccaggg attgccgctg ccttatttcg tcccatacag gaaaactcat ctgggtttct
     6661 gacaaatacg cgaaactcac agcccctcaa tatcaaccac acgttgtaag ccggtaatac
     6721 ctgaaaatca gcctattacc gcccacgcct ccggcaccat tcccttttga tttcgaattc
     6781 tttgttgtcg ctataccgct taatgttccc ggtcctccgg tgcagataac accggagtca
     6841 tcgtagcgct gcttcaatat tcaggtgacg tatttccaga cggaagccgg cgcgcgatcg
     6901 taaagtttgt tcattgtcag ttctgccagc cggttgtcaa cggcagagtt aaaggcttcc
     6961 agctccgcaa gggtaagttt ataacgcgtg tgggaattca cttttcccgc gtctcctgac
     7021 gtattacggg ctgccactta cctggtgtcc cgcaatacag ccttagtgtt ccgttatcgg
     7081 ggatccggac gcaccgcaac aaccggggaa gtatttctgg tttagagcag tgtcaagagg
     7141 gatttctcag cccctcactc accggctcag tctcagggca ggagcattaa tacgctcagt
     7201 tcatgtctta tacaggaatg ggcacagggg gagctggcgt gtcactgctg attttacgca
     7261 tcagcgttca tgtatctgca ctctgataca tgaacgattc acagcgctct gttaccggag
     7321 agatttactc tcttagctta ctctggcaaa tcctttacgt tactctctga tgacttctta
     7381 tatttataat accaatcaga cagccttatc ccatcggtga tatcagtctc ctcctcgcca
     7441 ggaacgggct gactcactgt aactacctcc gaaaacaact ctgcaattat cagaacgcct
     7501 gcttctctcc ctgtcctcac gaaaactatc ccctctttat cgcgcgtgcg tgcggaagca
     7561 tcttttcgca acaaccaccc ggtataccgg tacggctcag ccaccgcaac ctccccgttt
     7621 atctcctggc ggtcacattc ccgattattt tttacgtttc tccccggttg ttatgccggt
     7681 gaaggtggcg cgctgttttc atgaccacac cggctggttt aacaacatcc ggaggaacat
     7741 tctcatggct accccagttt cactgatgga tgaccagatg gtcgacatgg catttatcac
     7801 tcaactgacc ggcctgacag ataagtggtt ttacaggctc atcagggatg gggcctttcc
     7861 ggctcccatc aagctgggcc gcagctcccg ctggctgaaa agtgaagtgg aagcctggct
     7921 acaggcgcgt attgcacagc cccgcccgta atttctgacc gttatccgtt cacccgcagc
     7981 agacgtaatc cccggcctgc cggcggcatt ctgctgcctg ttatatcccc gtgaggaata
     8041 tgaaaatgaa acaacagtat cagacccgct acgaatggct ccacgaaagc taccagaaat
     8101 ggctgaccgg cttcacccgg cacgccgtat cctggggcgt gtgtcatccg aatatctact
     8161 acttccataa tctgacgccc ggttgggtgt cattcaatgg tgaaaaaccg gagattgcca
     8221 tcgtacccca gagcctgcac cggctgattt atggccctga caaacgggcc acgccgcccc
     8281 tggatgatga tctgatagtg aatttatgca ccagtgaaca tctgctggtt catcatccga
     8341 tgctggaagg cattctcctg tctgaatgtg aacgtctgag gcagcgttct ctggcaaata
     8401 aactgatcag cctcttccgt cagtttggtg gcacggagtt gcgcctcaaa ctggtctggc
     8461 tttgctggct tgatttaatg accggaaaca gccctgaaga ctggaaagag aacctgaaac
     8521 ggaaatcaga aaaagagctg gaggaatgga tcatcaaccg tcagaggcag agcgcagcac
     8581 tgacggatct gatggatcag tacgtgctcc tggcataccg cacaacggtt gacgatagcc
     8641 gcaactgatg ccggttgctt ccggaaggtg tgacaaaata cggaaatgat gatgctcccg
     8701 gcccccatgc tcacctgcag agcgtgttcg tcgtcatcat actgagccat gacgggcgtc
     8761 ttaagggcat cctgtcattg ctgagcaggc aacaccacca gaggagccac ggacagggat
     8821 gttctttaca gcccgataca cgccaggttc tcagcagact gaatgtgcag gtgcggttat
     8881 tcaccagaaa tggcatgctg cggtacatac ccatcctggt gacaatatgt acgtggcgac
     8941 agcgatcccg gcactgtgga agagactcac tcagcctgca ggatattctt tccggcatcc
     9001 gggaatgtcc gcctgaatgc aaacaggatg atatttgaat ccgtaatact gttctttcat
     9061 cataaataat ttatgcagat acagttaacc gttacagtag ccggtttgtt ttaccgggaa
     9121 ataatatccg tcagactgat accggaacat atctgaaaca cgttatgatg ttttgttatc
     9181 agaaatgttg tatattctta tccgtggcga cagtttaatg ccctctggta tcacctgata
     9241 atgtatatcc tgcgatgatt cgttatattt acgggacacg cctcttttca ttgtgctcct
     9301 tttcagactt cattttccgt aagtgttccc cggcataaag gaggtacctt tctggcgttt
     9361 ataactctct ttatcggtga cgccggtaat acagggaata ccacaccgac aaacgaggta
     9421 ttaacagacg atatttcagt gtccataatt tttctgtccg gttaaataac gtcagtctgc
     9481 tgccctctga ctgtaatcat caaagccgga atgcataccg ggttacaacg taaaaaaagt
     9541 gatgctggcc cccggtgcag ctggcgggcg aaacatgttt acctttcaat accccctgtc
     9601 tgcagtctcc actcttccat cctcttaagc tacgtcggcc acatgagtgc acttccatga
     9661 taacaggtat tcctgtcagc ataacgaagc cggttacctt cccttcctcc tgcatttcct
     9721 gtcagaatga ctatacagcg cgcagattgc ctttttattg accatcttaa tataccgtta
     9781 taaacttctt tttatcatat tattatcacc ttattatgtt taatttttaa gcagtcggag
     9841 aaagaggatg gttcagaaaa ttttgtcgga taaggttatg aatgaaagaa caaatgcgta
     9901 ttactcttat tatcttggag agcgtaatat ctccgtactg ccccttaatg tttatgatcc
     9961 cccggaacgt tttatcgctt acataaaaaa aaacagagaa aatctgaaca tcacactttc
    10021 agattttgaa cttgaacaaa taatttctgg tatgcgtctg aaggccctgg ccttcctggt
    10081 ccccctggaa aaaatatcct ggatcgcggg cagtgaacgc gcatgcttat tttcctggta
    10141 tctgttgatg cagtttatcc agaataacag ggcaaaaata agcgcagact tgcttcaaaa
    10201 gaataaactg tatcttaaag aagagtatct ggaagggaat gccttcccgt cggactcatc
    10261 gacacagttc agacaaatac tcagagttct cgatattctt tctgataaga atttgcggga
    10321 tgagtggata atccagacca aagacagatg gatgcgggca tttaaatcaa agagtccttt
    10381 cagttacctc ctcccggaga acgaacatga atgcatatgg acatggaatt atttgaaggg
    10441 aaaaaatatt gcactggaaa aactggccag ttttcctggt tctgctgata tttaccacgc
    10501 aattcacctt tcatttgaca tatgggttac ctgtccactc acctcacctg acgatataaa
    10561 aaatttcaga aacagtttca ataaggccaa ggcgcagcgc aagtacaaaa aaatgcagga
    10621 agataaagtc aatgtacagt tttttcttga cgcagaaacc agggcgcagc tcaaagaatt
    10681 atcgagggtc agaagactca gtaccggcga aatgctgcat gacctgattg ttgaagagta
    10741 taaaaggtac cgacattcca gataatttca gacatatatt atctccgtga gtcccccgcc
    10801 acctttccgg tgtgcggggg ttttgtcttt tttcaccggg aatacatgta tgaatccgtc
    10861 tgatgccatt gaggcaattg aaaaaccgct ctcctccctg ccttactcac tttcccgtca
    10921 catcctggaa catctgcgca aactcaccag tcacgaaccc gtgattggca ttatgggtaa
    10981 aagcggggcc ggtaaatcct cactctgtaa tgcactgttt cagggggagg tcaccccggt
    11041 cagtgatgtt cacgccggca cccgggaagt gcagcgtttc cgtctgagtg gccatggtca
    11101 cagcatggtt atcactgacc tgcccggggt gggcgagagc cgggacagag atgcagagta
    11161 tgaagccctg taccgtgaca ttctgcctga acttgacctg gtactgtggc tgattaaagc
    11221 cgatgaccgt gccctgtctg tggatgagta tttctggcga cacatcctgc accggggaca
    11281 tcagcaggtg ctgtttgtgg tgacgcaggc cgacaaaacg gagccctgcc atgaatggga
    11341 tatggccggt attcagcctt ctcccgcaca ggcacagaat attcgcgaaa aaacggaggc
    11401 ggtattccgt ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg
    11461 ggaactggat acgctggtca gtgcgctaat gacagcactt cccgaccatg ccgccagtcc
    11521 cctgatgacc cgcctgcagg acgagctgcg cacggaatct gtccggggtc aggcccgtga
    11581 acagtttacc ggtgcggtgg accggatatt tgacacggcg gagagcgtct gtgttgcctc
    11641 tgttgcccgt acggttctgc gtgccgtccg tgacacggtg gtctctgttg cccgcgctgt
    11701 atggaactgg attttcttct gaacagtctg ccagggaaat gcctgataac attacgttaa
    11761 ccaactgaaa acatgacaca aacccctcat actcctcctg tttctcttgc gtgaccgcct
    11821 tgtcgttaat agaataaaac tatcgataaa acagatatcg atcgttgcta tcgatcgttt
    11881 atagtggtta atggtctgcg aaaaggcagc tgaatctctt catcatgcag aacggaattg
    11941 cacacaacgg actgatactt ctctggcgga tgagagaggg gaagcattta tgtccgggaa
    12001 aaaccacttt atctgaccat gctgttttgt acctgccggt atccactttt gtgggtaccg
    12061 gcttttttat tcaccctctg taaggaaaag ctgatgaaac gacatctgaa caccagctac
    12121 aggctggtat ggaatcacat tacgggcacc ctggtggtgg cttccgaact ggcccgctca
    12181 cggggaaaac gcgccggtgt ggcggttgtg ctgtctcttg ctgctgtcac atcagtcccg
    12241 gcactggctg ctgacacgat tgtacaggcg ggagaaaccg tgaacggcgg aacactgaca
    12301 aatcatgaca accagattgt tctcggtacg gccaacggaa tgaccatcag tagcggtctg
    12361 gagtatgggc cggataacga ggccaatacc ggcgggcaat ggatacaaaa tggcggtatc
    12421 gccaacaaca ctactgtcac cggtggtggt cttcagagag tgaatgccgg aggaagcgtt
    12481 tcagacacgg ttatcagtgc cggaggcgga cagagccttc aggggcaggc agtgaatacc
    12541 actctgaacg gcggtgagca gtgggtacat gaaggcggga ttgcaacggg taccgtcatt
    12601 aatgagaagg gctggcagac cgtcaaatcc ggcgcaatgg caaccgacac ggttgtgaat
    12661 accggcgcgg aagggggacc agatgcagaa aatggtgata ccgggcagtt tgttcgcgga
    12721 aatgccgtac gtaccactat caatgaaaat ggtcgtcaga ttgtggctgc tgaaggaaca
    12781 gcaaatacca ctgtggttta tgccggcggc gaccagacgg tacacgggca tgcgctggat
    12841 accacactga atggcggtta ccagtatgtg cacaacggcg gtacggcgtc tggcactgtt
    12901 gtaaacagtg acggctggca gattatcaag gaaggtggtc tggcggattt caccaccgtt
    12961 aaccagaaag gcaaactgca ggtgaacgcc ggtggtaccg ccacacatgt caccctgaaa
    13021 cagggtggag cactggttac cagtactgcg gcaacggttc ttggcagcaa ccgtctgggc
    13081 aatttcacgg tggaaaacgg taaggctgac ggagttgttc tggagtccgg cggtcgcctg
    13141 gatgtactgg aaggccattc agcgcagaaa acacgggtgg atgacggcgg aaccctggca
    13201 gtgtctgccg gtggtaaggc aacaggtgtc accatgacat ccggtggtgc cctgattgca
    13261 gacagtggtg ccactgttga ggggaccaat gccagcggta agttcagtat tgatggcaca
    13321 tccggtcagg ccagcggact gctgctggaa aatggcggca gctttacggt taatgccgga
    13381 ggactggcca gcaacaccac tgtcggacat cgtggaacac tgacgctggc tgccggggga
    13441 agtctgagtg gcagaacaca gctcagtaaa ggtgccagca tggtactgaa tggcgatgtg
    13501 gtcagtaccg gcgatattgt taacgcaggg gagattcgct ttgataatca gacgacaccg
    13561 gatgccgcgc tgagccgtgc tgttgcaaaa ggcgactccc cggtaacgtt ccataaactg
    13621 accaccagta acctcaccgg tcagggtggc accatcaata tgcgtgttcg ccttgatggc
    13681 agcaatacct ctgaccagct ggtgattaat ggtggtcagg caaccggcaa aacctggctt
    13741 gcgtttacaa atgtcggaaa cagtaacctc ggggtggcaa cctccggaca gggtatccgg
    13801 gttgtggatg cacagaatgg cgccaccaca gaagaaggtg cgtttgccct gagtcgcccg
    13861 cttcaggccg gcgcctttaa ctacaccctg aaccgtgaca gcgatgaaga ctggtacctg
    13921 cgcagtgaaa atgcttatcg tgctgaagtc cccctgtata catccatgct gacacaggca
    13981 atggactatg accggattct ggcaggctcc cgcagccatc agaccggtgt aaacggtgaa
    14041 aataacagcg tccgtctcag cattcagggc ggtcatctcg gtcacgataa caacggcggt
    14101 attgcccgtg gagccacgcc ggaaagcagc ggcagctatg gcttcgtccg tctggagggt
    14161 gacctgctga gaacagaggt tgccggtatg tctctgacga caggggtgta tggtgccgca
    14221 ggccattctt ccgttgatgt taaggatgat gacggttccc gcgccggcac ggtccgggat
    14281 gatgccggca gcctgggcgg atacctgaat ctgacacaca cgtcctccgg cctgtgggct
    14341 gacattgtgg cacagggaac ccgccacagc atgaaagcgt catcggacaa taacgacttc
    14401 cgtgcccggg gctggggctg gctgggctca ctggaaaccg gtctgccctt cagtatcact
    14461 gacaacctga tgctggagcc acaactgcac tatacctggc agggacttac cctggatgac
    14521 ggccaggata acgccggtta tgtgaagttc gggcatggca gtgcacaaca tgtgcgtgcc
    14581 ggtttccgtc tgggcagcca caacgatatg agctttggtg aaggcacctc atcccgtgac
    14641 actctgcgcg acagtacaaa acaccgtgtg agtgaactgc cggtgaactg gtgggtacag
    14701 ccttctgtta tccgcacttt cagctcccgg ggtgacatga gcatggggac agccgccgcc
    14761 ggcagtaaca tgacgttctc accgtcccgg aatggcacgt cactggacct gcaggccgga
    14821 ctggaagccc gtgtccggga aaatatcacc ctgggcgttc aggccggtta tgcccacagc
    14881 gtcagcggca gcagcgctga aggttataac ggtcaggcca cgctgaatgt gactttttga
    14941 taatcaggtc actctccttc aggccccggt catcatgacc ggggcttttt actttcaggg
    15001 agcaacagat atgccgggtt gtacatcacg tttattgccc gaaggccctt tctcacgcga
    15061 acaggctgtt gccgtcaaaa cagcttaccg caatgtgttt actgaagatg accagggaac
    15121 atattcccgg ctggttatcc gtaatgctga aggtcagttg cgctggtgat gccggaacac
    15181 agcttaatac tcagctcgcc agttaagggc ttctcagaca ataaacgtca ttcatttcat
    15241 ctgtcaggcc gcgtcttctc cgggagacgc agctttttca tttattacac cagtaaatcg
    15301 taaccaccga taaggagcaa attatgcgac tagccagtcg ttttggttat gcgaaccaga
    15361 tacgccgtga ccgtccgctg acacacgaag aactgatgca ctatgttccc agtattttcg
    15421 gtgaagaccg gcatacttcc cgcagtaaac gatatgcgta cattcccacc atcaccgtac
    15481 tggaaagcct gcagcaggaa ggttttcagc cattcttcgc ctgccagacc cgcgtgcgcg
    15541 acccgggccg ccggggatac acaaaacaca tgctgcgtct gcggcgggcc ggagagataa
    15601 acggagaaca tgtccctgaa attattctgc tcaactctca tgacggtacc tccagctacc
    15661 agatgctgcc gggttacttc agattcgtct gccagaatgg ctgcgtctgc ggtcagtctc
    15721 tgggggaagt gcgtgttcca caccggggaa atgtggtgga gaaagttatc gaaggggctt
    15781 acgaagtggt gggggttttt gaccggattg aggaaaagcg tgatgccatg cagtcgctgg
    15841 tcctgccgcc accggcacgc caggcgctgg cacaggcggc actgacttac cgttatggtg
    15901 acgaacatca gccagtcacc accgctgaca ttctgacacc acgacgcagg gaggattacg
    15961 gtaaagacct gtggagcgca tatcagacca tccaggagaa tatgctgaaa ggcgggattt
    16021 ccggccgcag tgcaaaagga aaacgtatcc acacccgtgc cattcacaac atcgacaccg
    16081 atattaagct caaccgcgca ttgtgggtaa tggcagaaac gctgctggag agcctgcgct
    16141 gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg ccccggaggc
    16201 gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta cccgtctgct
    16261 ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg catctggcca
    16321 tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc gaatatcatt
    16381 catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc cagctcccga
    16441 cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaaa cattgtctca
    16501 gaacaccacc gcttcggcct gtgcaccgga gactggcctg caacaactgg ttgccactcc
    16561 cgtccctgat gaacagcgca tcagcttctg gccgcagcat tttggcctca ttccacagtg
    16621 ggtcaccctg gaaccccgtg tcttcggctg gatggaccgt ctgtgtgaag actactgcgg
    16681 tggtatctgg aatctgtaca ccctgaacaa cggcggggca tttatggcac ccgaaccgga
    16741 tgacgatgat gacgaaacat gggtactgtt caatgccatg aacggtaacc gcgctgaaat
    16801 gagcccggaa gccgccggca ttgctgcctg tctgatgacg tacagtcatc atgcctgtcg
    16861 tacggagtgt tatgccatga cggtccatta ttaccggctg cgggattacg ccctgcagca
    16921 tccggaatgc agcgccatta tgcgcatcat cgactgaatg gaggcaggga caatgcagca
    16981 gctttccttt ctgcccggaa acatgacgcc cggcgagcgc agtctcattc tgcgggccct
    17041 gcaaaccctg gaccgccatc ttcatgaacc cggcgtggcc ttcacctcca cccgtgcggc
    17101 acgggaatgg ctgattctga acatggcggg actggagcgg gaagagttcc gggtgctgta
    17161 tctgaacaac cagaatcagc tgattgccgg tgaaaccctc ttcaccggca ccatcaaccg
    17221 tacggaagtt catccccggg aagtgattaa acgcgccctg taccacaatg ccgctgccgt
    17281 ggtgctggcg cacaatcacc cgtccggtga agtcacaccc agtaaggcag accggcttat
    17341 caccgaacgt ctggtacagg cactgggcct ggtggatatc cgggtgccgg accatctgat
    17401 agtcggtggc aaccaggttt tctcctttgc cgaacatggt ctgctttaac ccgtcacaac
    17461 cacatcacac ctgttttcac ttttatcttc tgtcttcaga ggtatcacat catgagaatc
    17521 atcacccgtg gtgaagccat gcgtattcac caacagcatc ctgcatcccg tctttttccg
    17581 ttctgtaccg gtaagtatcg ctggcacggc agtgctgaag cgtatactgg tcgtgaagtg
    17641 caggatattc ccggcgttct tgccgtgttt gcagaacgcc gtaaggacag ttttggcccg
    17701 tatgtccggc tgatgagtgt caccctgaac tgatgttccg tcacccggga aacgagtacg
    17761 accagatgac ctcagacgcc gctgtggatg aggccattct catcaatgca tacatcttca
    17821 ccgaaaccgg tcagcgcgcc ggctgacctg tcatcgttaa accattctta accacttttc
    17881 agagaggatt ttatcgtgtc agacaaactc tccgggataa ctcatcccga tgacaatcac
    17941 gaccgcccct ggtgggggct tccctgcaca gtgaggccct gttttggcgc tcgtctggtg
    18001 caagagggta accgcctgta ttaccttgcc gaccgcgccg gtatcagagg ccgcttcagc
    18061 gacgcagatg cataccatct ggaccaggcc tttccgctgc tgatgaaaca actggaactc
    18121 atgctcacca gcggtgaact gaatccccga tatcagcata ccgtcacgct gtatgcaaaa
    18181 gggctgacct gcgaagccga caccctcggc tcctgtggtt acgtttatct ggctgtttat
    18241 ccgacaccag cacccgcaac cacctcataa ccccgaagct ctcaccttta gcactaccag
    18301 ttacagagaa cttaagatga aaacattatc cgacacacat gtacgggagg tatctcgctg
    18361 cccgtctccc gtcaccatct ggcagacact gctcatccga ctgctggacc agcattatgg
    18421 cctcacactg aatgacacac cgtttgtcga tgaacgtgtg attgagcagc atattgaggc
    18481 aggcatttca ctgtgtgatg cagttaattt tctcgtggaa aaatacgcgc tggtgcgtac
    18541 cgaccagccg ggattcagca cctgtccccg ctctcagtta ataaacagta ttgatatcct
    18601 ccgggctcgc cgggcgaccg gcctgatgac ccgcgacaat tacagaacgg taaataacat
    18661 taccctgggt aagtatccgg aggcgaaatg atgaaactgg ccctgacact ggaagccgac
    18721 agcgttaacg tacaggcact gaacataggg cgcattgtcg ttgacgtcga tggtattgag
    18781 ctcgctgaac tgattaacat ggtctgcgat aacggctact cccttcgtgt tgttgatgaa
    18841 tctgaccaga cctcagcaga atgcacgcca ccatttgcta cccttaccgg catacgctgc
    18901 agtaccgcac atatcacgga aacggacaac gcctggttgt actcgctgtc acaccagacc
    18961 agtgacttcg gtgaatcaga atggattcat tttacaggta gcggatatct gttacgtacc
    19021 gatgcgtggt catacccggt tctgcggctt aaacgcctgg ggctgtcaaa aacgttccgt
    19081 cgtctggtcg tcacactcat ccggcgttat ggcgtcagtc tcattcatct ggatgccagc
    19141 gctgaatgcc tgccgggttt acccactttc gactggtaac caggaacaac atgaaatcat
    19201 taaccacgga aacagcgctg gatattctga ttgcgtggct gcaggacaat atcgactgcg
    19261 gatcgggaat tatctttgac aacgatgagg ataaaacgga ttcagcagca ctgttgccct
    19321 gtatcgaaca ggcccgggag gatgtccgta ccctgcgtca tctgcagctt ctgcaccaga
    19381 accggtgaag ctcactcacc agacttcatt cctctgacgc cagcctgcac agggctggcg
    19441 ttttcattta tctgcaaaaa ggaatatcga ttatgtcaga tatcacaatc tcccgtccgg
    19501 aagtggtcaa cgggcatacg gacgttatct gctcaaccag cattcgccac attctggctg
    19561 tacgaaagag tacactgttg caaatcgaca cacttatccg acaactggct gaaatctcgg
    19621 tgctgacgga aagcatcgga ggtaaaactg ccccggactg ggccatgaaa caggattttc
    19681 gctgcggctg ctggctgatg gagaaacccg aaacagcaat gaaagccatt acccggaatc
    19741 ttgatcgcga aatctggcgt gacctgatgc aacgttccgg gatgctgtca ttaatggata
    19801 cgcaggcccg tgatacatgg taccggtcac tggagtacga taattttccg gaaatcagcg
    19861 aagcgaacat tctgagcaca tttgaacaac tgcaccagaa taaggatgag gtgtttgagc
    19921 gaggggtgat caacgtcttc agagggctga actggaatta caaaaccaat tgcccctgta
    19981 aatttggcag taaaattatc gtcaacaatc tggtgagatg ggaccggtgg ggataatgat
    20041 atggtcaccc ccaccttaac agcagtacgc tgttaaggta aatcccttta acggagagac
    20101 catcatgcag aacatcacac ttattggtat cgatctcgga aagcactcct tccacattca
    20161 ttgtcaagac aagtctggta aggctcttct gcgtaaaaaa tttacccgta ctaaactgat
    20221 gcagtttctg gcgacatgtc cgccctcagt tgttgtaatg gaagcctgtg cgggtgcaca
    20281 ctttatggct cgctgtatct ctgattttgg gcatgaaact aagctgattt ctccacagtt
    20341 cgttcgcccg tttgtcaaaa gtaataagaa tgattttgtt gatgcagaag ccatatgcga
    20401 agcggcttca cgaccatcaa tgcgttttgt ccagccaaga actgagaccc agcaggccat
    20461 gagggcgtta caccgggtca gagaatcgct catcagagat aaggtgaaaa caaccaacca
    20521 gatccacggt ttcttgctgg aatttggaat cagcctgccg ataggtgatg ctgtaataaa
    20581 acggttgtct ctcgtgttgg ctgaacacga aatccccgaa tatctgagcc gccttttggt
    20641 gaggttgcat acccactact tttaccttgt tgagcaaata gcagagctgg aatcagaatt
    20701 aagtcagtca atcaacgctg atgataccgc tcagcgcatt atgacaattc ctggtgttgg
    20761 tccgattact gccagcttac tatcgtcgca acttggtgac gggaaacagt tctcatgcag
    20821 cagggatttt gcagcttcca caggacttgt tccccggcag tatagcactg gaggaaaatc
    20881 gactctgttg ggtatcagta agcgaggtga taaaaattta cgccggttac tggttcagtg
    20941 tgcccgctca ttcataatgc gacttgaacg caagcatgga aaacttgctg agtgggttcg
    21001 ggagcaactc aacaagaagc attcgaatgt ggttacctgc gctctggcaa ataaactggc
    21061 tcgaattgcc tgggcaatca ctactcagaa aaaagaatat caggcctgat atagatgctg
    21121 atataactca attagtttat tttaaacaat aagatacaag ttacctttct ggttttgcga
    21181 agactgatta ttgatgacat gaacggcaaa tcgacctgat taaaaaccta acagatgaaa
    21241 tggctcactg aagccgaggg gatttttagg attatcaggt gcggaactca tcggggcgcg
    21301 ggcatccttg cccacagaga cgccggatag atttaagcaa gccaaccatt aatcaaaaat
    21361 cagtgttgca aaaacggggg tgaccataga tttcatctta tcaccggaca acaggctgac
    21421 cgactcgccg atctggaaag aatgttgcac ctgttcagcg gtaaaccaat ccccgacaac
    21481 cggggaaaca tcacccttcg tctggatgat cacattcagt ctgttcaggg taaagagagc
    21541 tatgaagatg agatgttcag catcagatac tttaagaagg gctccgcgca tatcacgttc
    21601 agaaaaccag aactggttga cagactgaat gatattatcg caaaacacta tcctgatatg
    21661 ctggcagtat agctttgcca gtatgctcaa agatcggaaa ttgtccgata agatatgatc
    21721 gcggggcata taaaccggtt tcagatggca tacccgatgg catactgtct ggaaggaaaa
    21781 ataaaaaagc gcatgttaaa caataacatg cacttataat ctggcggtga gggggggatt
    21841 cgaacccccg atacgttgcc gtatacacac tttccaggcg tgctccttca accgctcgga
    21901 cacctcacca aattgttgtt cctgtcttgc tggaacgggc gctaatttag ggaaatcatg
    21961 acctgaggtc aacaaacttt ttgaaaaaat cgcgcgttta ttcaaacttc aatcaatgtg
    22021 tggttttaat aagcgaaatc tgcttttttt gccaccgacc acggatttgt tatgctggtg
    22081 gcctttgtag atcataacga taagtgcgaa taaatttcgc acaacgcttt tcgggagtca
    22141 gtatggatat catcttttat cacccaacgt tcgataccca atggtggatt gaggcactgc
    22201 gcaaagctat tcctcaggca agagtcagag catggaaaag cggagataat gactctgctg
    22261 attatgcttt agtctggcat cctcctgttg aaatgctggc agggcgcgat cttaaagcgg
    22321 tgttcgcact cggggccggt gttgattcta ttttgagcaa gctacaggca caccctgaaa
    22381 tgctgaaccc ttctgttcca ctttttcgcc tggaagatac tggtatgggc gagcaaatgc
    22441 aggaatatgc tgtcagtcag gtgctgcatt ggtttcgacg ttttgacgat tatcgcatcc
    22501 agcaaaatag ttcgcattgg caaccgctgc ctgaatatca ttgggaagat tttaccatcg
    22561 gcattttggg cgcaggcgta ctgggcagta aagttgctca gagtctgcaa acctggcgct
    22621 ttccgctgcg ttgctggagt cgaacccgta aatcgtggcc tggcgtgcaa agctttgccg
    22681 gacgggaaga actgtctgca tttctgagcc aatgtcgggt attgattaat ttgttaccga
    22741 atacccctga aaccgtcggc attattaatc aacaattact cgaaaaatta ccggatggcg
    22801 cgtatctcct caacctggcg cgtggtgttc atgttgtgga agatgacctg ctcgcggcgc
    22861 tggatagcgg caaagttaaa ggcgcaatgt tggatgtttt taatcgtgaa cccttaccgc
    22921 ctgaaagtcc gctctggcaa catccacgcg tgacgataac accacatgtc gccgcgatta
    22981 cccgtcccgc tgaagctgtg gagtacattt ctcgcaccat tgcccagctc gaaaaagggg
    23041 agagggtctg cgggcaagtc gaccgcgcac gcggctacta ataaagcatc aggattcctg
    23101 ctatccttgg cgggaattga atacaggaga gagttatgta tcccgtcgac cttcatatgc
    23161 ataccgttgc cagcacacat gcatatagca cattgagtga ttacattgcc caggccaaac
    23221 aaaagggcat taaacttttt gcgatcaccg atcatggccc ggatatggaa gatgctccgc
    23281 atcactggca cttcattaac atgcgtatct ggccgcgagt ggttgatggg gtagggatcc
    23341 tgcgcggcat cgaagctaac attaaaaatg ttgatggtga aattgattgc agcggtaaaa
    23401 tgtttgactc gctggatctg attattgccg gttttcatga gccggttttt gcaccacatg
    23461 acaaagcgac caatacacaa gcgatgatcg ccactatcgc cagcggcaat gtgcatataa
    23521 taagccatcc cggaaatccc aaatatgaaa tagatgtgaa agccgttgct gaagcagccg
    23581 cgaaacatca ggtggcgctg gaaatcaata attcttcatt tttacactca cgtaagggca
    23641 gtgaagacaa ctgtcgtgcg gtagccgcag cggtacgtga tgctggaggt tgggtggcat
    23701 taggctcgga ttctcacacc gcgtttacca tgggggaatt tgaagagtgt cttaaaatcc
    23761 tcgacgcggt agattttccg ccagagcgca ttttgaatgt ttctccgcgc cgcttactga
    23821 acttccttga atctcgcggt atggcaccga ttgcggaatt tgcagacctt taattactag
    23881 taatggaaat atattaatga acgagttttc tatcctctgt cgtgtgctgg gttcgctcta
    23941 ttaccgccaa ccgcaagatc ctttactggt gccgctgttt accctgattc gtgaggggaa
    24001 actggctgcg aactggccac tggagcagga tgagttactg acgcgtttac agaaaagctg
    24061 tgatatggcg caagtctctg ccgattacaa tgcgttgttt atcggcgatg aatgtgccgt
    24121 gccgccatat cgtagcgcat gggttgagga cgcaacggaa gcggaagtgc gcgcttttct
    24181 ttccgaacga gggatgccac tggcggatac gccagccgat cacatcggca cattgctgct
    24241 tgcggcttcc tggatggaag atcagtcaac ggaagatgag agcgaagcac tggaaacact
    24301 gttcagtgag tatctgttac cctggtgtgg tgcgttcctc ggcaaagtgg aggcccatgc
    24361 aaccacgcct ttctggcgca ccatggcacc gctaacccgt gatgccatta gtgcaatgtg
    24421 ggacgagctg gaagaagatt ctgaagagta aatgtgatca tcgtcactca taactgcaac
    24481 agataatttg ttgttgcata aaatgtgtgc tcgatctcat tcatggccgc gttttctgct
    24541 atcatgtgcg gcatgaacat acttctctct attgcaatca caacaggcat tctctccggt
    24601 atctggggat gggtggctgt ttccctgggc ttactaagct gggcgggctt cctgggctgt
    24661 acggcctact ttgcctgccc gcaaggtggg ctgaaagggc tggcgatctc cgctgcaacc
    24721 ctgcttagtg gcgtggtgtg ggcgatggtc attatttacg gtagtgcact ggcaccacat
    24781 ctggaaattc tcggttatgt cataaccggt attgtggcgt ttctgatgtg tattcaggcc
    24841 aaacagctgc tgctttcatt tgttccgggg acgtttatag gcgcatgcgc gacgtttgcc
    24901 gggcagggtg actggaaact ggtgttacct tcgctggcgc ttgggctggt atttggttac
    24961 gcaatgaaaa acagcggcct gtggctggcg gcgcgtagtg caaagacggc tcaccgtgag
    25021 caggaaatca aaaataaagc gtgaggggca ctcacgcttt cgcttaaaca gtaaaatgcc
    25081 ggatgataat tccggctttt ttatctgtca ggattccggt ggaaccgaca tatggcggta
    25141 tttcaccaga atgtcattct gccgttctgc tttattttgc aaatcccaca gaccacggtc
    25201 gataccatca ttaatcagga aaatgacccc tgtttcgata gccgacatca gacacagcat
    25261 aacgggttcg tttgaggtgt aacccacttc cccttccagc aagcgctggt agtcaataaa
    25321 gcggaaaacc ccggcctgaa cttcatagga aagtatcgtc ttactggtgt tcaccgagga
    25381 gaggatctca ccggtactca cattgacgac acgcaggttc acggcaatct gatcgagctg
    25441 gtattgcgtg tctgcaccga tcccaaaata tcttgcccca acaccgcccg atttaacgtt
    25501 gctttcataa cc
//
LOCUS       DAJTNN010000042        25410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTNN010000042 DAJTNN010000000
VERSION     DAJTNN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.042"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1401)
                     /locus_tag="OO150_001541"
     CDS             complement(<1..1401)
                     /locus_tag="OO150_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5515730.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAF"
     gene            complement(1404..3071)
                     /locus_tag="OO150_001542"
     CDS             complement(1404..3071)
                     /locus_tag="OO150_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5515731.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(3068..5176)
                     /locus_tag="OO150_001543"
     CDS             complement(3068..5176)
                     /locus_tag="OO150_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5515732.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(5163..5984)
                     /locus_tag="OO150_001544"
     CDS             complement(5163..5984)
                     /locus_tag="OO150_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5515733.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(6144..7973)
                     /gene="glmS"
                     /locus_tag="OO150_001545"
     CDS             complement(6144..7973)
                     /gene="glmS"
                     /locus_tag="OO150_001545"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5515734.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(8135..9505)
                     /gene="glmU"
                     /locus_tag="OO150_001546"
     CDS             complement(8135..9505)
                     /gene="glmU"
                     /locus_tag="OO150_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5515735.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQESNTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(9857..10276)
                     /gene="atpC"
                     /locus_tag="OO150_001547"
     CDS             complement(9857..10276)
                     /gene="atpC"
                     /locus_tag="OO150_001547"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5515736.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(10297..11679)
                     /gene="atpD"
                     /locus_tag="OO150_001548"
     CDS             complement(10297..11679)
                     /gene="atpD"
                     /locus_tag="OO150_001548"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5515737.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(11706..12569)
                     /gene="atpG"
                     /locus_tag="OO150_001549"
     CDS             complement(11706..12569)
                     /gene="atpG"
                     /locus_tag="OO150_001549"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5515738.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(12620..14161)
                     /gene="atpA"
                     /locus_tag="OO150_001550"
     CDS             complement(12620..14161)
                     /gene="atpA"
                     /locus_tag="OO150_001550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5515739.1"
                     /translation="MQLNSTEISELIKLRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(14174..14707)
                     /gene="atpH"
                     /locus_tag="OO150_001551"
     CDS             complement(14174..14707)
                     /gene="atpH"
                     /locus_tag="OO150_001551"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5515740.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(14722..15192)
                     /gene="atpF"
                     /locus_tag="OO150_001552"
     CDS             complement(14722..15192)
                     /gene="atpF"
                     /locus_tag="OO150_001552"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5515741.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(15254..15493)
                     /gene="atpE"
                     /locus_tag="OO150_001553"
     CDS             complement(15254..15493)
                     /gene="atpE"
                     /locus_tag="OO150_001553"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5515742.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(15540..16355)
                     /gene="atpB"
                     /locus_tag="OO150_001554"
     CDS             complement(15540..16355)
                     /gene="atpB"
                     /locus_tag="OO150_001554"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5515743.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(16364..16744)
                     /gene="atpI"
                     /locus_tag="OO150_001555"
     CDS             complement(16364..16744)
                     /gene="atpI"
                     /locus_tag="OO150_001555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5515744.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(17361..17984)
                     /gene="rsmG"
                     /locus_tag="OO150_001556"
     CDS             complement(17361..17984)
                     /gene="rsmG"
                     /locus_tag="OO150_001556"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5515745.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(18048..19937)
                     /gene="mnmG"
                     /locus_tag="OO150_001557"
     CDS             complement(18048..19937)
                     /gene="mnmG"
                     /locus_tag="OO150_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5515746.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(20316..20759)
                     /gene="mioC"
                     /locus_tag="OO150_001558"
     CDS             complement(20316..20759)
                     /gene="mioC"
                     /locus_tag="OO150_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5515747.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(20849..21307)
                     /gene="asnC"
                     /locus_tag="OO150_001559"
     CDS             complement(20849..21307)
                     /gene="asnC"
                     /locus_tag="OO150_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5515748.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
     gene            21459..22451
                     /gene="asnA"
                     /locus_tag="OO150_001560"
     CDS             21459..22451
                     /gene="asnA"
                     /locus_tag="OO150_001560"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5515749.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYLDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(22456..23907)
                     /gene="viaA"
                     /locus_tag="OO150_001561"
     CDS             complement(22456..23907)
                     /gene="viaA"
                     /locus_tag="OO150_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5515750.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(23901..25397)
                     /gene="ravA"
                     /locus_tag="OO150_001562"
     CDS             complement(23901..25397)
                     /gene="ravA"
                     /locus_tag="OO150_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5515751.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARALEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         6297 a         6581 c         6023 g         6509 t
ORIGIN      
        1 aaaagcttgg ctaatccgtc taatttgata atcttccaca ctctctgagt aacgctttaa
       61 gcaaagcgct accaaaggca gtttctgtag attttttgca agagaggttc cgttaggagt
      121 ttgctttaac agccagctcg atgtcgctct tgggtgatca aggctactat ctagacgctt
      181 tatgattctt aatagttgtc gtacagcaat tcgatctctt tggttccaat caactctagg
      241 ggcgggtgcc agacgctctt gttgatgctg ttgattccag tgaactagcc aatccctgtc
      301 atgtcggtaa agccaagcgt atagcacccc accctctaaa gactgtcttg ccgccttaat
      361 tccttggtat ttatgcacta gttgctgcca gtctttatgc ttaacagata aatcttcaga
      421 gttaggttgc acagactggc taacaggtct tgtcgttata gagtgctcag taagagcact
      481 tgcctgctgt agcgcttcta taaccgttag ttttggcaat aaggcttgcc acacaatact
      541 atgctgtaaa taactaaagg cttttctatg cttacggaat atacttttca gccaacacgt
      601 gtccttgttc tctgccaact ttaaatccag tttctctagt gcctcatcac taaaagtctg
      661 cctcactctc tccgccacca agtcatgacg aatgtgcttg cttttggtta gccctagatc
      721 ctgcgctaag cgctgataaa acagcgtcca ctgctcaagg cttggggaaa gctcttgcgc
      781 tcgtggagca tctaacagag gggctatata agcagctagt gctgttaatt gagataggga
      841 gtctttgggg tagtctgaaa gcagctcagt atgacccaaa gcccaaaatt gatgtcggtg
      901 atcatctaca gctctatcaa agaagactaa agcaccgtgt tttggacaat atggcaaagc
      961 gggcaaatac caatctcgtt gccaaaaggc ttccccatac ctatttagct gaagagcaac
     1021 gcaatcaggg cagtatctaa agcggttatc gctcttaact ctagaagcag cgactcctag
     1081 cattaaatgc accgcacctt gcgcttggta ctccattaac cgaatagctt cgtctcggcg
     1141 ctccttgcct acaaacggag catataaagg gaataaggta tgctcataaa taagctgctg
     1201 aacagcgtaa cgtcctgttt gatgtagatg tcttgctatc acacctaaat gcgagggcag
     1261 acctaaggta gcgaccacct tgcggttgcc atacacctca tccaacagct gcttaggact
     1321 aacaatccct tgataaacgc ctgcccgtgc aatagtgcta taaatcagct cattcgagta
     1381 cggaacagga aagtttctca tggctaacct gcttgtttaa ataagctcgc catatccact
     1441 attacccctt tcccttttaa ccgttcatgc atggtttttt caggttggcg ttgtgaatag
     1501 atataacgta aatccgtatc aggcaagcta tcccagtcgc tgggcttgac tacctttata
     1561 gccgaaacct ttttactctt ggagggcttt tctagttctg acactaccgt ttcgccttcc
     1621 atcaaccact gcaaaacaag aggcagtaac ttttgtcttg tcatcgttgg attctggcta
     1681 aacgcttttt taatagtggg aattaacagg cttgaatcgt aatcctcttt cagcatcaga
     1741 tataaatgac gctgatcttc ggtatctaac tcttgaaggg ctttttcttc tggtgtttgt
     1801 tcttgtatcg ctgcgatatc tagctgaagt tggattaacc gtttatcaat ctcgggaacg
     1861 actagatcag aataacgagc aatgcgttct gggatacccg agcgtaatgc ctctagcatg
     1921 gggtgcacag gctttaactc atcttgatac acttgccgca ataaaccagc ggtaatacgc
     1981 tcattgccta aagctagcgc acggagctga gcgagtacaa aaagttttac tacaatgtcc
     2041 atcactcctt ggcttagctc ataccacaca tcacggacct catccgataa cagcgcatct
     2101 ttgcgttgta aaagctgtaa ttgccaaaga ttatccgtaa aagcgatcca ctcttgattg
     2161 ggctttccac gttgcgtttg ttgtatagga tcccagaata tagctccaaa ccctgcccct
     2221 ctacgtgcag accgcaaatc agcctcaaaa atctctcgtg ctttaggggt accaatcaac
     2281 atcactggta cgccaataat attcaccatc gtcacaaaaa agttcagcat ctcttgagat
     2341 ccacccgaac gagagcggct taaatgctga atttcatcaa taaccaacaa ccctaaagca
     2401 tgtgcattgg ctatttgcga catcaaagcc aacatggttt ctataccatg acgttttaag
     2461 ccataacgac gctcatagtt cgagcccaag gctcgatcca acgctctgaa aaaattcaag
     2521 cagatttctt ttagcgaacc attatgcgag cagtctattt tcaaatacac cacctgctct
     2581 acattgagtt cacgatggta aatcacctga ggatacgtgg ctagaatacg atgaagagag
     2641 gtcgtcttcc cactaccaga acaaccaatt aacaataagc tttgtgccgt agatcgtgcc
     2701 tcctcaaagc gaaatgtctc caactctccc gtttgaacac gctcataacc attttgtaaa
     2761 tgcttttgta aatctcctgt tttaggattt ctgcctacgt agccacctcg aatcatgacc
     2821 gaaatacgct cacttagtag caaatgcgta cctaatggct gaaaatagtc atctggaata
     2881 cgacaaatgg tatgagctct gataacacgg gacttttgca agtcagagga agtaagctgt
     2941 aaagaggatt tcagtgatgc agcactattc actgactctt gtaatggtgg taaggcctcg
     3001 ataaaaggat tatcacgata agcctctacc cccgtatcac gataaactgc ttgaatccgg
     3061 gtagcactca tgactcatcc ttttctggtg gatcctgaaa taattcaggc acgtatgtgg
     3121 gtaggctgta atcttcttga tcatccgctt ccactgcgtt gaaaggaata actttagcct
     3181 catcacctga agagcttggc ttcaaatgct ccgcacgttt tttacgctcc gaggtcacgg
     3241 cttctttttt attagtttta atctgcttaa tgcgtgttga tttgggctca gtagtactgg
     3301 gcgttaactt attcgctttc tgaatggttt gctgaataaa cgcctcaagc tccctgcgtt
     3361 tagttaactc atcctgcttc gcattggctt tattgtgttt ttcttgtgct tgtatatccc
     3421 aaacctccca aaatgagaga cctttaaact gccgactacg ttccgtcaga ttacagcgcc
     3481 aaaatacacg gctgccaact tgcggaaaca aataaatcgt atcaaccagc actgggtcat
     3541 aagccgcttc taaatgttga ggtctagcta tatcagtgct ccgctgcaac caaccctcac
     3601 gcagaatctc tgaccccgag taatacaaac cccacaaatt aacgccaaat gaagaaatag
     3661 agacctttcg gcgaggcagt aacgctactc gcaactgctc ttgctccaca gcccttaaac
     3721 tacctgtacg atgctgcata ccccattgcc atagctggac aggaatagac ggtaaatctg
     3781 taggaaaatc agcatctcga tcgtatttat ccatcaccag atggttattt ctgaataaga
     3841 tcgtacgcaa aataatttgt gtgaactcaa ataccgacag agatgcatct aacctatagt
     3901 ctgtttcacc atggcttttg atccgactgc cctctacaat gccaggtgca aaggacttaa
     3961 actcggcttg tagtgttcta aaagtgcttt ccactatgcc tttagcatcg ccacgtctag
     4021 gtggagcact ttccactcgc acattaaaac tagaaactaa ggcttcgacc tgatgactca
     4081 ttaattcgcc acggtccgct agcaacacat ctggcaaacc tacacacggc cagtctgagc
     4141 tactaatctc aatatcatgc tgggcacaaa tggccgtttt gtcagagcaa gcatttacaa
     4201 aagcctgcat cgccaccaca taagacggat tttcaaagcc gatataaaag cccgtgatca
     4261 tccgactaaa cacatcaatc acaatgtaaa gcgttggtct tcctatgatt ttttggcgat
     4321 catgatgatc cactaaataa atatcagcaa tcgtggcatc aatctcataa cgactcccag
     4381 ggcctaacgc ctgagaagtg gctgtactac ttaagggtcg tacgtctttt ttatataccc
     4441 ctgctttaac tctagacttt aagcgctgag ctttagggta ttctcgatca taaaaataac
     4501 gaaactgacg tagtgttggg taatcctctt ggggaatgcg aggaaaatac tgagcaaaca
     4561 agtccacaaa tcgtctatag gcaacggtgg tctttgtacc tttttgattt aacaggtgct
     4621 tttctatggt caacctaaaa aggcgttcaa tctcgggcgt taccttggtt ccttcaccct
     4681 taccatattc tctggctcgg ccaatctttg ctgttcctgt cgctgaacgt ctttcccctg
     4741 gtgcaccgct gtttttgtag tcaggaatta atgcattagg cgtttgacca cgctgccagt
     4801 aacggcgtaa caacttataa actgtagcct tagtaacctt atgttcttgg accacatgct
     4861 caacgagttc gcttctgact ttagggtcga aacgatcctt actattaata ataggaagaa
     4921 tttttcgata atcctcctcg cgcttctgga agctgacaga atctacagac ggctcttcta
     4981 aatcaagatg tacataaggg tcatcagttc taactaagcg gccttcatct aaatactgca
     5041 ttagcaacaa ctcagcccta gcttgtggaa ctcctttatc agcgcttatt tgcatccaga
     5101 caacttggcc atcctctata gccaaaatgc gatacggatc attatcaaat agcacaacct
     5161 cattaatttg ccacatagcg caactcctcc atattcacta cttggctaat acagagatct
     5221 gcacacttat ttgccctgaa agacttataa atattgaact taataaaacc atttgcggtt
     5281 aaggctcgta tctcactcaa tgttttgcct aactccaaat cataagcaat atcaacctgc
     5341 ttacagacat tgataatgtt ttcatctcct ttttcttgca ggatatgggc caatggggat
     5401 agttgtgcta aaagctccgc agaaacttct tctgttttca ctgaataaag ccattcaata
     5461 ttttctttta ctacgggatt tatttcttta tcagtaaaaa tgaaccaagg aatttgcttt
     5521 tgctgccaat agcgacgctc tagttctagt ttttctaagg tacgctcgtc ttgtaaggct
     5581 gctgcaggtt tgacttgaat agcaaactgc tcaaaaggac catctttgca gtccactaaa
     5641 aaatcagtag acataacctg atctacacca cgaataacag gatgcttaat accactatct
     5701 attgcaatct gcctggtatc actaggtaat aaggggaact gctcgcgtat atctagcacg
     5761 ctgctctccc actcaagact gagaaaaaca gcaagctcta agtcagatag caaatgatgg
     5821 actcgtcccg tcttatgaga ataaatacgg tgggaacgac ctgaagaagg aacttcttgt
     5881 actgttagcc atggaatata gtctttacca tgcccttggc cacgcccctc tttaatacga
     5941 cgggcaattt gcacttcaga aaatgaagag tttgctttag ccataacaaa agtccagtat
     6001 gctttttcac agcataactg gactgatttc agtttacaac tattctgtct agtttaagac
     6061 tttattgtca tagtttagat ctattttgtt cagtttaaga ctttattgtc cgcccacaag
     6121 ccctttttta tctatgatag tgattactca accgtaaccg attttgccaa gttacgcggc
     6181 tggtcaacgt cggtgccttt gatcagcgcg acatggtaag ccagcagctg cagcggaacg
     6241 gtgtagaaga tcggtgcaat cacctcttcc acatgcggca tctcgatgat gtgcatgtta
     6301 tcgctactta caaaacccgc atcctgatcg gcgaagacat acaactgacc gccacgcgcg
     6361 cgaacttctt caatgttgga tttcagtttt tccaacagtt cgttgttcgg tgcaacgacg
     6421 ataaccggca tatccgcgtc aatcagcgcc agcgggccgt gtttcagctc gccagcagcg
     6481 taggcttcag cgtgaatgta ggagatctct ttcagcttca atgcgccttc cagtgcgatt
     6541 gggtactgat cgccacggcc caggaacagt gcgtgatgtt tgtcagagaa atcttctgcc
     6601 agagcttcaa tgcgtttgtc ctgagatagc atctgctcaa tacggctcgg cagcgcctgc
     6661 agaccatgca caatgtcatg ttcaatggag gcatccagac ctttcaggcg agacagcttc
     6721 gccaccagca tcaacagcac agttaactga gtggtgaatg ctttagtgga cgccacgccg
     6781 atttccgtac ccgcgttggt catcagcgcc agatcggatt cgcgcaccag agaagaaccc
     6841 ggaacgttac agattgccag cgaaccaagg taacccagct ctttcgacag acgcaggccc
     6901 gccagggtat ccgcggtttc gccagactgt gacaaggtga tcatcaggct gttacgacgc
     6961 acggcagatt tacgatagcg gaattcagag gcgatttcga cgtcgcacgg aatacctgcc
     7021 agcgattcaa accagtagcg ggaaaccata ccggagttat aagaagtacc acaggcgagg
     7081 atctgaatat gctcaacctt cgacagcagt tcgtcggcgt tcggtcccag ctcgcttaaa
     7141 tcaacctgac cgtggctgat gcgcccagta agggtgtttt tgatcgcgtt cggctgttcg
     7201 tagatctctt tctgcatgta gtgacggtaa atacctttat cgcccgcgtc atattgcaga
     7261 ttggattcga tatcctgacg ttttacttcc gcgccagttt tatcgaagat gtttaccgaa
     7321 cggcgagtaa tttccgcaat atcgccctct tcaaggaaga taaagcgacg ggtcaccggc
     7381 agcagcgcca gctggtcaga agcgataaag ttttcgccca tacccaggcc aatcaccagc
     7441 ggactaccag aacgtgccgc cagcagggta tccgggtgac gggagtccat gatcactgta
     7501 ccgtacgcac cgcgcagctg cgggatagca cgcagaacag cctcacgcag agtcccgcct
     7561 tgttttagct cccagttcac cagatgggca atcacttcgg tgtcggtttc agaaacgaag
     7621 gtatagccac gcgcttttaa ctcttcacgc agcggttcat ggttttcgat gatgccgtta
     7681 tgcaccacca taatgtgttc agaaacatgc ggatgcgcgt tagcttctga aggttcaccg
     7741 tgggtcgccc agcgagtgtg agcaataccg gtgccgccat gcagaggatg ttcttccgct
     7801 gcctgagcca gcatctggac tttaccgagg cgacgcaggc gggtcatatg accttccgca
     7861 tcaacaacgg ccagaccagc agagtcatat ccgcggtatt ccagacgacg taaaccttca
     7921 agaaggattt ctgctacatc acgttgcgcg atcgcgccaa caattccaca catagttttt
     7981 gattccgatt tatatcgttg tcggtcaacc tgtatgcccg ttttttcggg cgccccgagc
     8041 cttgtagaga gtggggttat ttttatagtt actgcttgtg ggagggagat tatgttatct
     8101 cctcatccca gatgaccgga ttacccggcc agaattactt tttctttacc ggacgacgcc
     8161 agccttcttt ctgagtctgc ggcacacggc tgatagctaa tgcattttcg ccgacattac
     8221 gcgtcacagt tgtacccgca gcaatggtcg cgcctttgcc tactgttacc ggggccacca
     8281 gctgagtgtc ggaaccgaca aacacatcgt cgccgataat ggtcttaaat ttattcgcac
     8341 catcgtagtt gcaggtaatg gttcccgcgc cgatgttaac gttatcgcca atttccgcat
     8401 cgcccaggta agtcagatga ccagctttcg agcctttacc cagacgcgct tttttcatct
     8461 caacaaagtt accgacgtgt gcaccttcca gcaactcagc accaggacgc agacgggcaa
     8521 acgggccaat agtacaggcc gctgccagat tcacgtcttc cacaacggta tacggactga
     8581 tttcgcaatc atcgccaatc acgctgtttt taatcacgca accggtgccg attttcacgc
     8641 gatgaccgag agtcacgttg ccctcgatga taacgttagt atcaatttca acatcgcgcc
     8701 cgtgagtaag cgtaccgcgc agatcaaaac gcgccggatc gcgcagcata acgcctgcta
     8761 acagcagttt ttcagcctgt tcggactggt aaacgcgctc cagacgggag agttgcaggc
     8821 ggttattcac accttctact tcgcttaaac gttgcggatg aacggcgacg atttcacgcc
     8881 cttcctgata cgccagcgca ataatgtcgg tgatgtagta ttcgccctga gcattattgt
     8941 tggtcagctt cgccagccag cgtttcatat ctgcgccgtt agagatcaga atgccggtgt
     9001 tgctctcctg aatctgacgc tgctcgtcgg tggcatcttt gtgctcaaca atgccggtaa
     9061 ctttgccatt ttcacgggtg atacgtccat aaccggtcgg atcatccagt ttcaccgtca
     9121 gcagaccaat gccaccctgc ggtttagcat cacgcaggcg ctggagtgtt tcgacagaga
     9181 tcagcggcac gtcgccgtag agcattaaaa tgtcttcatc atcggcaaag aaaggtgcgg
     9241 cctgctgcat tgcatgaccc gtacccagct gctctgcctg aagcacccag ttaaggttgt
     9301 cgtctttcag cgcctgttta agcagatcgc cgccgtgacc gtacaccagg tgaacgtgcg
     9361 ctgcgcctaa ttcattcgca gcatcaatga catgctgaac catcgctttc ccggcaaggg
     9421 tatgcagcac tttcggaaga tcggaataca tgcgcgtgcc tttgcctgcg gcaaggatca
     9481 ctacgctcat agcattattc aacatacgcg tcctgactgt aatttgagaa cgaatttaaa
     9541 ccgcttcacc ttgaaaaaac tacatttttt tcatcgtgaa atggacagag gataaattgt
     9601 tcaataacgg attatccccg ctgacgaaag tgccattttc gaccatcgtc acgccttttg
     9661 tctgcagaaa attaaggcag taccttcatt taagcgtcag gtggatgttt ttgctcttat
     9721 ttcgatcaat gaataaacgg gaaaacaggg gtgttttata tcagcaggat ctatgtgaac
     9781 gctcttcagg acggatcacg cgtaaaaaaa aaaccagcct gtttccagac tggcttttgt
     9841 gcttttcaag ccggtgttac atcgcttttt tggtcaactc gataacgcgc agctgcgcga
     9901 tcgctttggc cagttccgca gacgcctgag cgtaatctac gtcgccgtga gagctgctaa
     9961 tgtgctcttc agccttacgt ttcgcttcca tggctcgcgc ttcgtcgaga tcctggccgc
    10021 gaattgcggt gtcggccaga acggtcacgt tgccagactg cacttcaaga atgccgccag
    10081 acagatagat aaactcttcg tgaccgtgct gtttcacgat gcgaatcata ccaggcttaa
    10141 tggcggtgag cagcggtgcg tggccagggt agatccccag ttcaccttcg ctacccgtta
    10201 cctggatttt ctcgaccaga ccagagaaca tttgttgctc tgcgctgacg acgtccaggt
    10261 ggtaagtcat tgccatatca ccctccgatt aaggcgttaa agttttttgg ctttttccac
    10321 agcttcttcg atggaaccga ccatgtagaa cgcctgctcc ggcaggtgat cgtattcgcc
    10381 ttccatgatg cctttaaagc cacggatggt gtctttcagg gagacgtatt tacccggaga
    10441 accggtgaat acttctgcca cgaagaacgg ctgggacagg aagcgctgga tcttacgagc
    10501 acgcgctacc accagtttgt cttcttcaga cagttcatcc atacccagga tggcgatgat
    10561 gtctttcagt tcctgataac gttgcaggat ggactgaacg ccacgcgcgg tgtcgtagtg
    10621 ttcctgacca accaccagcg ggtccagctg acggctggtg gagtccagcg ggtcaacggc
    10681 cgggtagata cccagagacg cgatctgacg gctcagtacc acggttgcgt caaggtgcgc
    10741 aaaggtggtt gccggagacg ggtcagtcaa gtcatccgca ggtacgtata ccgcctgtac
    10801 ggaggtgata gaaccagttt tggtggaggt gatacgttcc tgcagaacgc ccatctcttc
    10861 cgccagggtc ggctgataac ctaccgctga aggcatacgg cccagcagtg cggatacttc
    10921 cgtaccggcc agggtgtaac gatagatgtt gtcaacgaac agcagaacgt cacgaccttc
    10981 gtcacggaat ttctcagcca tggtcagacc ggtcagagca acgcgcagac ggtttcccgg
    11041 cggctcgttc atctggccat acaccaggga tactttgtcg ataacgttgg agtcggtcat
    11101 ttcgtggtag aagtcgttac cctcacgagt acgttcacct acgcccgcaa acacagagta
    11161 accggagtgc tcgatcgcga tgttacgaat gagctccatc atgtttacgg ttttacctac
    11221 acccgcacca ccgaacagac caactttacc gcccttagcg aacggacaca tcaggtcgat
    11281 aactttgata ccggtttcca gcagttcctg agagtttgac agctcttcgt aggaaggtgc
    11341 tgcgcggtga atcgcccaac gctcttcttc accgatctcg cctttcatgt cgaccggttc
    11401 acccagtacg ttcatgatac ggcccagagt cgctttacct accgggactt cgatcgggtg
    11461 ttcgaggtct tttacatcca gaccgcgacg cagaccgtcg gaggaaccca ttgcgatggt
    11521 acgcacgata ccgccgccga gctgctgctg aacttccagc accagacgct cattaccatt
    11581 ttgcacctca agagcatcgt acacgcgcgg tacggcatcc tgagggaatt cgacgtcaac
    11641 tacggcgccg attacctgga caatctttcc agtagccatc ttaaatcctc tacgaaataa
    11701 cctgtttaaa ccgcggcggc ccccgagacg atctcggtga gttcctgagt aatgctggcc
    11761 tgacgagctt tgttgtatac caactgcagc tctttaatca ggctgccgcc attgtcggtc
    11821 gcggctttca tcgccaccat acgggcggcc tgctcgctgg ccaggttttc aaccacgccc
    11881 tgataaacct gagattcgac ataacgacgc agcagggtat ccagcaacgc cttcggatcg
    11941 ggttcgtaca ggtaatccca ggatttatgt ttcagatcat catcatctga tgccggtaac
    12001 ggcagcagct ggctgatggt cggaacctga gacatggtgt taataaattt gttgctgaca
    12061 atgtaaagct tgtccagacg gccttcgtcg taggcctgca acatcacttt taccggaccg
    12121 atcagttcgg acagggaagg gttatccccc atgccggtga cctgggcaac aacattgccg
    12181 cccacggagt tgaagaacga cacgcctttc gagccgatca ttgcgaggtc gcattgaacg
    12241 cctttgtcgg tccaggtctt catttccgcc agcagttttt tgaacaggtt aatgttcaaa
    12301 ccaccgcaca aaccacggtc ggtcgacacc accaggtagc ccacgcgttt aacgtcgcgg
    12361 tcttccaggt aaaggtgctt atattccaga ttaccatgtg caaggtgacc aatcactttg
    12421 cgcatggttt ctgcataagg acggctggcc gccatgcgat cctgcgattt acgcattttg
    12481 gaagcggcga ccatctccat cgctttagtg atcttttgcg tgttctggac gcttgcgatc
    12541 ttactacgta tctcttttgc gccggccatg agcttctcct caatgccttg cggcctgccc
    12601 taaggcaagc cgccagacgt taccaggatt gggttgcttt gaaggaatcg aggatgcctt
    12661 tcagtttgcc ttcgatttcg tcgttgtagc caccggtctg gttgatctct tgcatcaacg
    12721 gagcgtgatc acggtcgacg taagccagca gagcggcttc gaagctgcca attttcgaca
    12781 gttcaacatc cgccaggtaa ccacgttctg ctgcgaacag aaccagagac tgctgcgcaa
    12841 cggacatcgg cgcatactgt ttctgtttca gcagttcggt cactttctga ccgtggtcaa
    12901 gctgcttacg tgttgcatcg tcaaggtcgg atgcaaactg agagaacgct gccagttcac
    12961 gatactgtgc cagagcggta cggataccac cggacagttt tttcatgatc ttggtctgtg
    13021 ctgcaccacc aacacgggat acggaaatac ccgggttaac cgcaggacga ataccggcgt
    13081 tgaacaggtt ggtttccagg aagatctgac catcggtaat ggagattacg ttggtcggaa
    13141 cgaacgcaga aacgtcaccc gcctgagttt cgataatcgg cagtgcggtc agagaaccgg
    13201 ttttcccttt cacttcacct ttggtgaagg cttcaacgta ttcggcgtta acacgtgcag
    13261 cacgctccag cagacgagag tggaggtaga aaacgtcgcc cgggaatgct tcacgtcctg
    13321 gcggacgacg gagcagcagg gagatctgac ggtaagcaac agcctgttta gacaggtcat
    13381 cgtaaatgat cagcgcatct tcaccgcggt cacggaagta ttcgcccatt gcgcaaccgg
    13441 catacggtgc caggtattgc agtgcagcgg attcagacgc ggttgctacc acaacgatgg
    13501 tgttagccag tgcgccgtgc tcttccagtt tacgtaccac gttagaaatg gtggacgctt
    13561 tctggccgat agcgacatag atacatttga taccggaatc gcgctggttg atgatggcat
    13621 cgatagccag tgcggtttta cctgtctgac ggtcaccgat gatcaattca cgctgaccac
    13681 gaccgattgg gatcatggag tcaacggctt tataaccggt ctgtaccggc tgatctacgg
    13741 actgacgttc gataacgccc ggagcgattg cttctacagc agagaagccg tcgtgatcca
    13801 gcggaccttt accgtcgatt ggtgcaccca gagtgttaac cacacggccc agcaggccac
    13861 ggccaaccgg aacttccagg atacggccag tacacttaac tttcatgcct tcggcaaggt
    13921 cagcgtacgg acccataaca accgcaccta cagagtcgcg ctcgaggttc agtgcgatag
    13981 cgtaacggtt acccggcagg gagatcattt caccctgcat acaatcggcc aggccgtgaa
    14041 tgcggataac accgtcactt acagaaacaa tagtaccttc gttgtgagct tcactcacaa
    14101 cattgaactg agcaatgcgc agcttgatca gttcgctgat ttcggtggaa ttcagttgca
    14161 tgctccagtc cccttaagac tgcaagacgt ctgcaaggcg ctcaagacga ccgcgtacgc
    14221 tgccatcaat gaccatatca cccgctcgga tgataacgcc tgccattaca gacttatcga
    14281 ttttgcaatt cagcttaact ttgcgtgaca gacgtttttc catcgcagca gaaattttcg
    14341 cgagctgttg ttcactcagt gcggcagcgg aaatgacgtc tacctcagcg gtagcctcac
    14401 tcacggcacg aaggtgaata aactgctcca gaacatccgg gagcgcgtta agacgaccat
    14461 tttcagccat tacccgaatc aggttctgac cgttttcgtc cagttgctca ccacaaactg
    14521 cgataaacga ctcggcgagc gtttctggcg caagcgcgcc agagagaagc tctgccattt
    14581 gttcgttttt ggttacctcg gcggcaaacg ccagcatgtc ctgccagcgt tctacacttt
    14641 ggtgttcgac ggcaaagtca aaagctgctt tggcgtaggg gcgagctacc gtaataaatt
    14701 cagacatcag cccctccctc cttacagttc agcgacaagt ttatccacga tgtcgctgtt
    14761 agcagcttca tccacggaac gttcgatgat cttctcggcg ccagcaacag ccaggatagc
    14821 aacttgctta cgcagctctt cacgggcacg tttacgctcg gcttcaattt ctgcctgcgc
    14881 ctgggccacg attttagtac gttcctgttc tgcctcagct ttcgcttcgt ccagaatctg
    14941 cgagcggcgt ttgttcgcct gctcgatgat tacctgggct tccgctttcg cttttttcag
    15001 ctggtcggtc gcgctggcct ttgcaaggtc aaggtcctta tgtgctcgtt ctgcggaagc
    15061 aaggccgtca gcaatttctt tttgacgttt ttcgatggct gccattaatg gcggccatac
    15121 gtacttcatg cagaacagaa cgaacaggac aaacgcgatg gcctggccga ggattgttgc
    15181 gttaagattc acagcacaat gcctctattt agttaacgtt ctgatattgc tctttaaata
    15241 aaagcaacgc ttactacgcg acagcgaaca tcacgtacag acccagacct acagcgatca
    15301 tcgggatagc atccaccaga cccataacga taaagaactg agtacgcagc agaggaatca
    15361 gatcaggttg acgcgctgcg ccttccaaga atttaccccc gaggatgccg ataccgatcg
    15421 cagcaccgat tgccgccaga cccatcatca cagcggcagc catgtacagc agatccatat
    15481 tcaggttttc catgacagtc tccagtttgt ttcagttaaa acgtagtagt gttggtaaat
    15541 taatgttctt cagacgccat cgacagatag acgatcgtca gaaccatgaa gatgaaggct
    15601 tgcagcgtaa tgatcaggat gtggaaaatg gcccacggca cattcaggat ccactgtgac
    15661 caccacggca acagaccagc aatcagaatg aaaatcagct caccggcata catgttaccg
    15721 aacagtcgca aaccgagtga aactggtttg gacagcaggc ttaccccttc aaggattaag
    15781 ttgacaggaa tgaacgccca gtgattgaac ggctgcagcg tcaactcttt cgtgaagccg
    15841 ccgatgcctt tcattttgat gctgtagaac agaatcagga taaatacgcc caatgccata
    15901 gacagcgtta cgttcacgtc cgcagacgga accacacgca gtgcaggcag acccagtaca
    15961 tgttcagcaa tgtacggcag caggtcgata ggcagtaaat ccatcaggtt catcaggaat
    16021 acccagacga agatcgtcag ggccagcgga gcaatcagct tgcttttgcc atggtacatg
    16081 tctttcacgc taccattaac aaagccgatc accagctcaa tcgcggtctg aaacttacct
    16141 ggcacaccgc tggtcgcctt tttggctacg ctacggaata aaaccaggaa caacagaccc
    16201 agcaccaccg agaagaacat ggagtcaata ttgattgtcc agaaggtggc tggggggttt
    16261 tgtggatcca ccagcgagaa tgtacgcagg tccagctgaa ggttattcag gtggtgtcct
    16321 atgtaatcct gcggcgtcat attttctgaa gccatgatgc cttttaccct ttgttgttaa
    16381 ttacagccgg tgccagtatc tgaaccacca gcaccaaaac ccacgtaacg atcagcggca
    16441 agaataccgc ctttaaaacc gccaacgcca ccaccagtaa caccaacatc gccagaactt
    16501 tgaaagcttc gccaaatgcg aatgtccagg ccacccggcc tttcgctggt gtatgcgcct
    16561 ggtgacgcca ggcaaatatc ataaacaaaa cgttaggcag aaagactgcc aggcccccgc
    16621 ttattgcaga gacgccccag aaggggtctt tgaggctgaa cagcaatcca cttgctatca
    16681 ccaccagtaa ctgaacgagc agaagcttcc gagcaacgtt tcgactcacg agcgacacag
    16741 acatcacgtt ttttactcct gctcccttcg aggtatgccg cgtgtcgtat aaaactttct
    16801 ttaaggctta gagtcaagca tcaaaaagcg gtcaaattat acggcgcgcc cccgtgattt
    16861 caaacaataa gtagccaaaa ggtgaataaa tgtttaaata tttttccagt gcatacaatt
    16921 gcgacttttc tactaaccct gttcgatcat gaaaaactgt aaataacgcg taaacactgg
    16981 tgataaagcg tgcttcagat cacatattgc gcatgttcgc gcacagcata tttatttact
    17041 tggcaaatga tgcctttgca agtttatgat atttcagtct aaaaacagat actgttttaa
    17101 taaatgacat ttacacaaca aaaaccaccc attgacattt ttaataatgt tttagcagcc
    17161 aatgatggtt cttagcgccg atttttagca gactgatatt ttcactaatg acttattttc
    17221 tgcttaccaa aaaaagccac gttatcttgt tgatgcaaaa gagtgaacgt ggcgttaaat
    17281 gtaaccagtt atatcagtag aaaacctggt tgttgttaac agtctaaccg gtcaattttt
    17341 tatgattttt ttgataaaaa ttaaatttta tttgctttaa tcaccaccag atgacgttcg
    17401 ccatccaggg ctggaacctg aagtttaacc actgattcga cctgatattc ttcgggcaac
    17461 aaagcgattt catcttccgg catttgccct ttcagcgcgt agaaacggcc ttgctcacca
    17521 ggaagatggt ggcaccagct caccatatcg ttcagagagg caaaagcgcg gctaattacg
    17581 ccatcaaatg gcggctctga aggaaactct tctaccctgc tctgtacagg ttcaatattc
    17641 tccagtttaa gctcatgttg cacctgacga aggaaacgca cgcgtttacc aaggctatcc
    17701 aacagagtga aatgggcttc aggacgcacg atagagagtg gaatgcctgg cagtcctggt
    17761 ccggtgccga catcgataaa ccgttcacct tgcagatacg gtgccaccac aatgctatcg
    17821 agaatatggc gtaccagcat ctcattagga tcgcggaccg aagtcaggtt gtacgctttg
    17881 ttccatttat gcagcatatt cacgtaggca ataagctggt ttttctggtg atcggtaagc
    17941 gaaatacctg cgtctttcag cagtaaggag agtttgttga gcacggtgat tacctgttct
    18001 tgatgcgttg cctggtaagc gggtgcttac caggcatttt taatgcgtta tgcgctacga
    18061 cgcagcatac cctgtttttt cagccacacc agcagaatgg agatagccgc aggcgtgacg
    18121 ccagaaatac gcgaagcttg gccgatagag gctggtttgt gatcgttaag tttggcgatc
    18181 acttcgttag aaagaccgga tacctggcgg taatccagtg tcgcgggtag cagggtgttc
    18241 tcgttacgca gctgcttttc gatctcatct tgctggcgcg cgatataacc ttcgtattta
    18301 acctgaatct caacctgttc cgccgcctgt tcgtctgtca acgcaggggc aaacggcgtc
    18361 agcgtggtta atttttcata agtcatttcc ggacgacgca gcagatcttc accactggct
    18421 tcacgggaaa gcggcgcagt caggtgagca ttcacttcgg ctgcagcttc cgccgacggg
    18481 gttacccagg tcgatttcag acgctgacgc tcacgctcga tattctcaag tttctcgtta
    18541 aagcgcgccc aacgttcgtc atccaccagg cccagttcac gaccgatttc agtcaaacgc
    18601 agatccgcat tatcttcgcg tagcatcaga cgatattctg cgcgcgaagt aaacatacga
    18661 tacggttctt tggttcctaa agtgcacagg tcatcaacta gtacgccgag atacgcctga
    18721 gaacgtgccg gagcccaacc ttctttgtca gcagacagac gggcagcgtt aagaccggcc
    18781 agcaaacctt gcgcagcggc ttcttcgtaa ccggtagtgc cgttaatctg accagcaaag
    18841 aacagcccct ggataaactt gctctccagc gtcggtttca ggtcgcgagg atcgaagaag
    18901 tcatactcaa tggcataacc cggacgcacg atcttcgcgt tttccatccc ctgcatagag
    18961 cggacgattt gcatctgcac atcgaacggc aggctggtgg agataccgtt cggataaatt
    19021 tcattagagg tcagtccttc cggttcaagg aagatctgat gctgatttct gtcggcgaag
    19081 cgcatgactt tgtcttcgat cgacgggcag tagcgtgggc cgacaccttc gatcacccct
    19141 gcatacattg ggctacgatc gaggttactg cggatcacat catgggtttt ctcgttggta
    19201 tgagtgatat aacacggcac ctgctgggga tgctgggacg cattgcccat aaacgagaat
    19261 accggcattg ggttatcgcc atgctgttgc gccagcacac taaagtcgat agttcgagca
    19321 tcaatacgcg gtggtgtccc ggttttcaga cgaccaacgc gcagcggcag ttcacgcaaa
    19381 cggcgagaaa gcggaatgga cggcggatca ccagcacggc caccgctgta attatccaga
    19441 ccgatatgaa ttttaccgtc gaggaacgtc ccaacggtga gcacgactgc tttggcacgg
    19501 aacttcagtc ccatttgggt aacagcaccg accacgcgat cgttttcgac aataagatct
    19561 tcaaccgcct gctggaagat catcaggttc ggttggttct ccagcgccgt acgtaccgcc
    19621 tgacggtaga gcacacgatc cgcctgagct cgggtagcgc gaaccgccgg tcctttgctt
    19681 gcgtttagta tcctaaactg gatacccgct tgatcgatcg ctttcgccat cagaccgccg
    19741 agtgcatcca cttcttttac cagatgtccc ttcccaatac cgccgatcgc cgggttgcag
    19801 ctcatctgcc ccagagtgtc gatattgtgt gtcaaaagca gagtctgttg acccatacgc
    19861 gccgcggcca tcgcggcctc ggtgcctgca tgacccccgc caatgatgat gacgtcaaaa
    19921 ggatccggat aaaacatggt gattgcctcg cataacgcgg tatgaaaatg gattgaagcc
    19981 cgggccgtgg attctactca actttgtcgg cttgagaaag acctgggatc ctgggtatta
    20041 aaaaggagat ctatttattt agagatctgt tctattgtga tctcttatta ggatcgcact
    20101 gccctgtgga taacaaggat ccggctttta agatcaacaa cctggaaagg atcattaact
    20161 gtgaatgatc ggtgatcctg gaccgtataa gctgggatca gaatgagggg ttatacacaa
    20221 ctcaaaaact gaacaaccgt tgttccttgg ataactaccg gttgaaccaa gcttcctgac
    20281 agagttatcc acagtagaac gcacgatctg tatacttatt tgagtaaatt aatccacgat
    20341 cccagccatt cttctgccgg atcttccgga atgtcgtgat caagaatgtt gatcttcagt
    20401 gtttcgcctg tctgttttgc accggaattt ttgagttctg cctcgagttt atcgatagcc
    20461 ccacaaaagg tgtcatattc acgactacca ataccgattg cgccaaagcg gaccgcagaa
    20521 agatcgggct tctgttcctg caatgcttca tagaaaggag aaaggttgtc cggaatatct
    20581 ccggcaccgt gggtggagct gataatcagc cagatccccg aggcagataa atcttccaac
    20641 agcggaccgt gcagcgtttc ggtggtaaaa cccgcctctt ccagcttttc agccaggtgt
    20701 tctgctacat attcggcacc gccgagggtg ctgccgctga taagagtgat atctgccata
    20761 aaccgccacc tttattaaga gtggcgtatt gtacgctgtg aacgcgttgg gatctacctg
    20821 tggaaaagta tgggattaaa aaagccgatc agggcttgat agtacgcatg atcgggtgtt
    20881 gcaggacgat caatgtctcg gtggactgaa tttcatcaat tgtttggatc ttgttgataa
    20941 gtacatgctg gagagcgtcg atcgaacggc acattacttt tataaagatg ctgtagtggc
    21001 ctgttgtgta ataggcttca gtgacttcat caaggctttc cagctttgcc agcgcggaag
    21061 ggtagtcttt ggcgctcttt aatataatgc cgataaagca gcctacgtca taaccgagct
    21121 gcttcgggct gacatcaata cgcgccccgg taatgatccc cgcctgcttc attttctcta
    21181 cccgaacgtg aatcgtcccc ggactgacgc caaattgttt cgccagttcg gcgtaagcgg
    21241 tgcgcgcatt gcccattaat gcttccagga tgccacggtc cagattgtcg atcagataat
    21301 tttccatagg attttcttat gcagattgat gattcattct attttagcct tcttttttaa
    21361 tgaatcaaaa gtgtgttagg ctttttattg aatgattatt gcatgtgtgt cggtttttgt
    21421 tgcttaatca taagcaacag gacgcaggag tataaaaaat gaaaaccgct tacattgcca
    21481 aacaacgcca aattagcttc gtgaaatctc acttttctcg tcaactggaa gaacgtctgg
    21541 ggctgatcga agtccaggca ccgattctta gccgtgtggg ggatggcacg caggataact
    21601 tgtcgggctg tgaaaaagcg gtgcaggtaa aagtgaaagc tctgcctgat gcccagttcg
    21661 aagtggttca ttcactggcg aagtggaaac gtcagacctt agggcaacac gacttcagcg
    21721 cgggcgaagg gctgtacacg cacatgaaag cccttcgccc cgatgaagac cgtctttctc
    21781 cgttgcactc ggtctatgta gaccagtggg actgggaacg cgtaatgggc gacggtgagc
    21841 gtcaattctc gactctgaaa agcacggtag aggcgatctg ggcgggaatt aaagcaaccg
    21901 aagctgcggt tagcgaagag tttggcctgg caccgttcct gccggatcag atccacttcg
    21961 tacacagcca ggagttactg tctcgttatc tggatctcga tgccaaaggg cgtgagcggg
    22021 cgatagcgaa agatcttggc gcggtattcc ttgtcgggat tggcggcaag ctgagcgatg
    22081 gtcatcgcca cgacgtgcgc gcaccggatt atgatgactg gagcaccccg tcagagctgg
    22141 gtcatgcggg tctgaacggc gatattctgg tgtggaaccc tgtactggaa gatgcgtttg
    22201 agctttcttc catggggatc cgcgtggatg ccgacacgct gaagcatcag ctggcgctga
    22261 ccggtgatga agatcgtctg cagctggaat ggcatcaggc gctgctgcgc ggtgaaatgc
    22321 cgcagaccat cggcggcggt atcggccagt ctcgtttgac tatgctgctg ctgcaactgc
    22381 cgcatatcgg ccaggttcag tgtggagtat ggccagctgc tgttcgcgag agcgtccctt
    22441 ctctgctgta ataatttatc gccgccagcg tctgagcagg cggcttcgca tcccggtatc
    22501 aaagcgccag atatgatcga aaatgcgcat gatgccgggt ttgccgtgtg ccgacatcgc
    22561 cacggcatga aagcgatgct gatgtacccg ctgcagctct ttcactttac tcgtcacgtc
    22621 gtcaggcaac cgctgagcga taaaatcaga aatcaccacc gcatcggcat caaaccattc
    22681 cctgctttgc aagcgttcca taatggcgcg aaaacaactg gcaagatcgg tgccgccacg
    22741 aaactgctgg cttaaaaaac ggattgcttg ttcgatgcct tgtgggcctg aaagctcata
    22801 acggacgatc tcggtggaaa atagcataat atagcagcgc cggttttctg cgagagcaat
    22861 gcgcatcaag gccaggcaga acgctttcgc acactgttca ttaaagccgc ccattgagcc
    22921 ggaagtatcc acacagacaa taaacggccc gcgcggctgt tcatcgtaat ctttatgtac
    22981 caccggacgt tcgatcactt tttcacgcca cgactcaccg tgcaggcgat aggtgagcaa
    23041 ctgtttttcc accagccgac ggtaaaactc atactccagt tccgttatcc ctagtgtcgc
    23101 cagttctggc ggcaggagac gtaaaatatc atcgctttgt tgcagaccat caacctgctc
    23161 aggaaccgtc gccggttcgc gcaccatggt gcggaaggtt tccatctgcg catcgttgcg
    23221 cggtattgat ttggcttccc gagaacgccc cagctgctct gccaggcgtt tcagttccgg
    23281 ctgttcgtta agaaattcac cgtatttcac aatcaactga tagtcgccac gtttaagctg
    23341 gccggcgctc atatcccaca gacgaccagc tgcggtattg ttatctgcga gaatcggttc
    23401 aagttgtccg ctcagcgtca tgcgttcctg aacttcactt aacagttgtt cgcgttcttc
    23461 ttctaatagc tgttgattta acgtcgttgc ttgcacgatc agacttaaac gccagcgctg
    23521 gagaaaaagc gtgtgtaacg ctgaagttat tgtgctgtta gcatcaacca actgccgggc
    23581 ttgttctgcc catggggaat tcagacgatg cagtaagtcc aggatctgtg gtagctgcac
    23641 aataaactgc ggcgtggaga ggagctggct ttgctgatag cacatcacct cttcggtgag
    23701 ttctggcggg actcgggcat ctttcagccg actgcgcagc gcctcacgcc agcggggaac
    23761 atcatcagtg attgctgcct tcagtcgtgg gaatttttca aagaagaccg ccagctgcgg
    23821 tgaggccagc agtgcgatga tcatctcttc gatcaatccc tcttcgctga cggccagcat
    23881 cacattaagc gtatccagcg ttagcattgt tgtgcctggc gaatctgtgc accgacatcc
    23941 tgtaggctgg cttcaatgcg acctaaccag tcgccaggga taaacagaca tttttgctgt
    24001 tcgctgaata aagcgtgttg cttacgccag tcgctttcaa gctcttccag ttgttgtttt
    24061 atttcacctg gcagaccttc agccgatgaa ccgggaagtg ccagcgtact gccttgtaaa
    24121 cttacatcgc gtacaacaag atgttgggtg ctatcaactt ccagattcag tttctgggca
    24181 aagccgatac cgttcagttt gccgcgaatt tcaccacctt tactcagcca ctgttccagc
    24241 gcgctacgct caaaggagat atgaaccact tccatatcat gcagttttaa cggtttttgc
    24301 agcagcagag tcagagtgga agcagtaacg ttaacaggga gttgatactg ctgacgacgg
    24361 ctaaaaatgc cgcccagacg aattaccgtt aaggctgttt tatcgctttg ttgctgctga
    24421 agttgcaggt gacgttgcac aatcgcgcct aggcgggtca acatcccttg ctgttgccag
    24481 gcgtgaccgg tcatcaatac atcaatttgt tgttgtatca aattcaggct ttgcgcgtca
    24541 taccacaggc aatctttcag caaaatgaga tctaccgggg caacagcact gcgaccgcta
    24601 aaaaaggcgc tggcctgcaa taatcggatc gcttttttcc agcgacgatc cgagacataa
    24661 ggcgcatccg gtaatttatc cagttgctgg cgcagcataa aaatcagctc aaatacatga
    24721 tcgggcagcg taatttcacc aatctctttc tgccagcgtt catattcttc atctgtgacc
    24781 tgcaaggcat caggaaccgg attgtcgttt tcatcctgtt gactggtcag catggagcgg
    24841 aaattcgctt tatcctgcac tttatctaac cacagacgaa tcagcatgcg gtcatataac
    24901 gcttccagac tgctgtctgc ttccggcagc tcgttggagg ccgccaccag cagacgcatc
    24961 gggatttttt ctacgtgtgc accgttgcgg aactggcgct cgttaatggc ggtgagcaag
    25021 gtattaagaa ttgccgggcc cgctttccag atctcatcca gaaagacaat ttctgcttcc
    25081 ggcaggtaac cgctggttaa acgttcatag cgcccttcat cttttagcgc ctgaatagaa
    25141 aggggaccaa aaacttcttc cggcgtggag aagcgggtca tcagatattc aagcgcgcgg
    25201 gcattctgaa aggcgaattt taagcgccgg gcgatcaaac ttttggcaat acctggcggg
    25261 ccaaggagga acacactttc accacttaat gccgctaata aacacaagcg gatggcgtgg
    25321 ctacgttcat aaagcccctt ttccaacgaa ctgctcaggc gggaaattct ttccgctaat
    25381 aaatgagggt gagccataat gaagtggcgt
//
LOCUS       DAJTNN010000043        25141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTNN010000043 DAJTNN010000000
VERSION     DAJTNN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.043"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..403)
                     /locus_tag="OO150_001563"
     CDS             complement(<1..403)
                     /locus_tag="OO150_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5515752.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLP"
     gene            782..1093
                     /gene="rpsJ"
                     /locus_tag="OO150_001564"
     CDS             782..1093
                     /gene="rpsJ"
                     /locus_tag="OO150_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5515753.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1126..1755
                     /gene="rplC"
                     /locus_tag="OO150_001565"
     CDS             1126..1755
                     /gene="rplC"
                     /locus_tag="OO150_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5515754.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1766..2371
                     /gene="rplD"
                     /locus_tag="OO150_001566"
     CDS             1766..2371
                     /gene="rplD"
                     /locus_tag="OO150_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5515755.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2368..2670
                     /gene="rplW"
                     /locus_tag="OO150_001567"
     CDS             2368..2670
                     /gene="rplW"
                     /locus_tag="OO150_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5515756.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2688..3509
                     /gene="rplB"
                     /locus_tag="OO150_001568"
     CDS             2688..3509
                     /gene="rplB"
                     /locus_tag="OO150_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5515757.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3526..3804
                     /gene="rpsS"
                     /locus_tag="OO150_001569"
     CDS             3526..3804
                     /gene="rpsS"
                     /locus_tag="OO150_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5515758.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3819..4151
                     /gene="rplV"
                     /locus_tag="OO150_001570"
     CDS             3819..4151
                     /gene="rplV"
                     /locus_tag="OO150_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5515759.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4169..4870
                     /gene="rpsC"
                     /locus_tag="OO150_001571"
     CDS             4169..4870
                     /gene="rpsC"
                     /locus_tag="OO150_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5515760.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4883..5293
                     /gene="rplP"
                     /locus_tag="OO150_001572"
     CDS             4883..5293
                     /gene="rplP"
                     /locus_tag="OO150_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5515761.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5293..5484
                     /gene="rpmC"
                     /locus_tag="OO150_001573"
     CDS             5293..5484
                     /gene="rpmC"
                     /locus_tag="OO150_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5515762.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5484..5738
                     /gene="rpsQ"
                     /locus_tag="OO150_001574"
     CDS             5484..5738
                     /gene="rpsQ"
                     /locus_tag="OO150_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5515763.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5903..6274
                     /gene="rplN"
                     /locus_tag="OO150_001575"
     CDS             5903..6274
                     /gene="rplN"
                     /locus_tag="OO150_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5515764.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6285..6599
                     /gene="rplX"
                     /locus_tag="OO150_001576"
     CDS             6285..6599
                     /gene="rplX"
                     /locus_tag="OO150_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5515765.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6614..7153
                     /gene="rplE"
                     /locus_tag="OO150_001577"
     CDS             6614..7153
                     /gene="rplE"
                     /locus_tag="OO150_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5515766.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7168..7473
                     /gene="rpsN"
                     /locus_tag="OO150_001578"
     CDS             7168..7473
                     /gene="rpsN"
                     /locus_tag="OO150_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5515767.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7507..7899
                     /gene="rpsH"
                     /locus_tag="OO150_001579"
     CDS             7507..7899
                     /gene="rpsH"
                     /locus_tag="OO150_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5515768.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7912..8445
                     /gene="rplF"
                     /locus_tag="OO150_001580"
     CDS             7912..8445
                     /gene="rplF"
                     /locus_tag="OO150_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5515769.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRIKEAKKK"
     gene            8455..8808
                     /gene="rplR"
                     /locus_tag="OO150_001581"
     CDS             8455..8808
                     /gene="rplR"
                     /locus_tag="OO150_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5515770.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8823..9326
                     /gene="rpsE"
                     /locus_tag="OO150_001582"
     CDS             8823..9326
                     /gene="rpsE"
                     /locus_tag="OO150_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5515771.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9330..9509
                     /gene="rpmD"
                     /locus_tag="OO150_001583"
     CDS             9330..9509
                     /gene="rpmD"
                     /locus_tag="OO150_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5515772.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9513..9947
                     /gene="rplO"
                     /locus_tag="OO150_001584"
     CDS             9513..9947
                     /gene="rplO"
                     /locus_tag="OO150_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5515773.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9955..11286
                     /gene="secY"
                     /locus_tag="OO150_001585"
     CDS             9955..11286
                     /gene="secY"
                     /locus_tag="OO150_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5515774.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11318..11434
                     /gene="rpmJ"
                     /locus_tag="OO150_001586"
     CDS             11318..11434
                     /gene="rpmJ"
                     /locus_tag="OO150_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5515775.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11581..11937
                     /gene="rpsM"
                     /locus_tag="OO150_001587"
     CDS             11581..11937
                     /gene="rpsM"
                     /locus_tag="OO150_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5515776.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11954..12343
                     /gene="rpsK"
                     /locus_tag="OO150_001588"
     CDS             11954..12343
                     /gene="rpsK"
                     /locus_tag="OO150_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5515777.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12377..12997
                     /gene="rpsD"
                     /locus_tag="OO150_001589"
     CDS             12377..12997
                     /gene="rpsD"
                     /locus_tag="OO150_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5515778.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13023..14012
                     /gene="rpoA"
                     /locus_tag="OO150_001590"
     CDS             13023..14012
                     /gene="rpoA"
                     /locus_tag="OO150_001590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5515779.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14053..14436
                     /gene="rplQ"
                     /locus_tag="OO150_001591"
     CDS             14053..14436
                     /gene="rplQ"
                     /locus_tag="OO150_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5515780.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14543..14911
                     /locus_tag="OO150_001592"
     CDS             14543..14911
                     /locus_tag="OO150_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR5515781.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGF
                     STDFLRGDYADKLLDKINDN"
     gene            14922..15347
                     /gene="zntR"
                     /locus_tag="OO150_001593"
     CDS             14922..15347
                     /gene="zntR"
                     /locus_tag="OO150_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR5515782.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15403..15621
                     /gene="arfA"
                     /locus_tag="OO150_001594"
     CDS             15403..15621
                     /gene="arfA"
                     /locus_tag="OO150_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR5515783.1"
                     /translation="MSRYQHTKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="OO150_001595"
     CDS             complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="OO150_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR5515784.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="OO150_001596"
     CDS             complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="OO150_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5515785.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="OO150_001597"
     CDS             complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="OO150_001597"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5515786.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRHH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="OO150_001598"
     CDS             complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="OO150_001598"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5515787.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERYIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19853..20362)
                     /gene="def"
                     /locus_tag="OO150_001599"
     CDS             complement(19853..20362)
                     /gene="def"
                     /locus_tag="OO150_001599"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5515788.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20492..21616
                     /gene="dprA"
                     /locus_tag="OO150_001600"
     CDS             20492..21616
                     /gene="dprA"
                     /locus_tag="OO150_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5515789.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     YALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVSPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21588..22061
                     /locus_tag="OO150_001601"
     CDS             21588..22061
                     /locus_tag="OO150_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5515790.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22090..22632
                     /locus_tag="OO150_001602"
     CDS             22090..22632
                     /locus_tag="OO150_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR5515791.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22637..23209
                     /gene="tsaC"
                     /locus_tag="OO150_001603"
     CDS             22637..23209
                     /gene="tsaC"
                     /locus_tag="OO150_001603"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5515792.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVLGETGGRLNPSEIRDALTGELFRQG"
     gene            23214..24032
                     /gene="aroE"
                     /locus_tag="OO150_001604"
     CDS             23214..24032
                     /gene="aroE"
                     /locus_tag="OO150_001604"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5515793.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24029..24286
                     /locus_tag="OO150_001605"
     CDS             24029..24286
                     /locus_tag="OO150_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5515794.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24262..24816)
                     /locus_tag="OO150_001606"
     CDS             complement(24262..24816)
                     /locus_tag="OO150_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5515795.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGGVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6421 a         5976 c         6549 g         6195 t
ORIGIN      
        1 aaggcagcgg gttttttaat gattcttttc ctctcatgag cagaccaata actacggctc
       61 cgcaagcgaa tgaggcagca aggaaaacca gtcgtggtaa gaaggcccat gaatgccagg
      121 cgccaagggc ggcgagaaac ttcacatcac catagcctaa tccttcttta tgtcgcagta
      181 tgcgataccc ccagtaaatg acagcgaatg tgccgtaacc aacaattgtc ccccataacg
      241 catcggctaa accatcagga tgacaaactt gatagaacaa aagacctgac cagagtaacg
      301 ggcacgtaaa tctgtcaggt aacaagccat gctttgcatc ccagaaaaaa agtagggctg
      361 ataaacaagc gtacaaaatc agaaagggaa gggtagtgtc cataaaacat cctctgtaat
      421 tggagtatca ccatcgaata actccggatg ctcagcaaca gagagagcgc tgatttgtta
      481 acctgattcc ggtgaaaaac gctatcttca caaccttaca aatttcgtgg aatgcagctc
      541 gcaatctgcg cgttgcgatt aatttctacc cgatgcttgc gtcaggctca gatttgcgta
      601 taatgcgcgg gcttgtcgta gttgacagca ggttcaatct gaacctcagt tagccgaatt
      661 tggctaccta acaatgctcc caatcgggga gctacgtaag aacggttaca ctctcccatc
      721 aatcgtaatg ggtctgagga gtaatcattt tcgtttataa aataattgga gctctggtct
      781 catgcagaac caaagaatcc gtatccgcct gaaagcgttt gatcatcgtc tgatcgatca
      841 agcaaccgcg gaaatcgtcg agactgccaa gcgcactggt gcgcaggtcc gtggtccgat
      901 cccgctgccg acacgcaaag agcgcttcac tgttctgatc tccccgcacg tcaacaaaga
      961 cgcgcgcgat cagtacgaaa tccgtactca cttgcgtctg gttgacatcg ttgagccaac
     1021 cgagaaaacc gttgatgctc tgatgcgtct ggatctggct gccggtgtag acgtgcagat
     1081 cagcctgggt taatcaggtc attgagcgat tgagaggttg aaacaatgat tggtttagtc
     1141 ggtaaaaaag tgggtatgac ccgtatcttc acagaagacg gcgtttctat cccagtaacc
     1201 gtaatcgaag ttgaagcaaa ccgcgttact caggttaaag acctggctaa cgatggctac
     1261 cgtgctattc aggtgaccac cggtgctaaa aaagctaacc gtgtgaccaa gcctgaagct
     1321 ggccacttcg ctaaagctgg cgtagaagct ggccgtggtc tgtgggaatt ccgcctggct
     1381 gaaggcgaag agttcactgt aggtcagagc attagcgttg aactgtttgc tgacgttaaa
     1441 aaagttgacg taactggcac ctctaaaggt aaaggtttcg caggtaccgt taagcgctgg
     1501 aacttccgta cccaggacgc tactcacggt aactccttgt ctcaccgcgt tccgggttct
     1561 atcggtcaga accagactcc gggcaaagtg ttcaaaggca agaaaatggc aggtcagatg
     1621 ggtaacgaac gtgtaaccgt tcagagcctt gacgtagtac gcgttgacgc tgagcgcaac
     1681 ctgctgctgg ttaaaggtgc tgtcccgggt gcaaccggta gcgacctgat cgttaaacca
     1741 gctgtgaagg cgtaaggaga tagcaatgga attagtattg aaagacgcgc agagcgcgct
     1801 gactgtttcc gaaactacct tcggtcgtga tttcaacgaa gcgctggttc accaggttgt
     1861 tgttgcttat gcagctggtg ctcgtcaggg tactcgtgct cagaagactc gtgctgaagt
     1921 aactggttcc ggtaaaaaac cgtggcgcca gaaaggcacc ggccgtgcgc gttctggttc
     1981 tatcaagagc ccgatctggc gttctggtgg cgtgaccttt gctgctcgtc cgcaggacca
     2041 cagtcaaaaa gttaacaaga agatgtaccg cggcgcgctg aaaagcatcc tgtccgaact
     2101 ggtacgtcag gatcgtctga tcgttgtcga gaagttctct gtagaagcgc cgaaaactaa
     2161 gctgctggca cagaaactga aagacatggc tctggaagat gtgctgatca tcaccggtga
     2221 gctggacgaa aacctgttcc tggctgcgcg caacctgcac aaggttgacg tacgcgatgc
     2281 aactggtatc gacccggtta gcctgatcgc cttcgacaaa gtcgtaatga ctgctgatgc
     2341 tgttaagcaa gttgaggaga tgctggcatg attcgtgaag aacgtctgct gaaggtgctg
     2401 cgtgcaccgc acgtttctga aaaagcgtct actgcgatgg aaaaatccaa caccatcgta
     2461 ctcaaagttg ctaaagacgc gaccaaagca gaaatcaaag ctgctgtgca gaaactgttt
     2521 gaagtcgaag tcgaagtcgt taacaccctg gtagttaaag ggaaagttaa acgtcacgga
     2581 cagcgtatcg gtcgtcgtag cgactggaaa aaagcttacg tcaccctgaa agaaggccag
     2641 aatctggact tcgttggcgg cgctgagtaa gtcggaggag taatacaatg gcagttgtta
     2701 aatgtaaacc gacatctccg ggtcgtcgcc acgtagttaa agtggttaac cctgagctgc
     2761 acaagggcaa accttttgct ccgttgctgg aaaaaaacag caaatccggt ggtcgtaaca
     2821 acaatggccg tatcaccact cgtcatatcg gtggtggcca caagcaggct taccgtattg
     2881 ttgacttcaa acgcaacaaa gacggtatcc cggcagttgt tgaacgtctt gagtacgatc
     2941 cgaaccgttc cgcgaacatc gcgctggttc tgtacaaaga cggtgaacgc cgttacatcc
     3001 tggcccctaa aggcctgaaa gctggcgacc agattcagtc tggcgttgat gctgcaatca
     3061 aaccaggtaa caccctgccg atgcgcaaca tcccggttgg ttctactgtt cataacgtag
     3121 aaatgaaacc aggtaaaggc ggtcagctgg cacgttccgc tggtacttac gttcagatcg
     3181 ttgctcgtga tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag
     3241 aagcagactg ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc
     3301 tgggtaaagc aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc ggtaccgcga
     3361 tgaacccggt agaccaccca catggtggtg gtgaaggtcg taactttggt aagcacccgg
     3421 taactccgtg gggcgttcag accaaaggta agaagacccg cagcaacaag cgtactgata
     3481 aattcatcgt acgtcgccgt agcaaataat tttagaggat aagccatgcc acgttctctc
     3541 aagaaaggtc cttttattga cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc
     3601 ggagacaaga agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc taacatgatc
     3661 ggtttgacca tcgctgtcca taatggtcgt cagcacgttc cggtatttgt aaccgacgaa
     3721 atggtcggtc acaaactggg tgaattcgca ccgactcgta cttatcgcgg ccacgctgct
     3781 gataaaaaag cgaagaagaa ataaggtagg aggaagagat ggaaactatc gctaaacatc
     3841 gccatgctcg ttcttctgct cagaaggttc gccttgttgc tgacctgatt cgcggtaaga
     3901 aagtgtcgca ggctctggat attttgacct acaccaacaa gaaagcggct gtactggtca
     3961 agaaagttct ggaatctgcc attgctaacg ctgaacacaa cgatggcgct gacattgacg
     4021 atctgaaagt tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc attatgccgc
     4081 gtgcaaaagg tcgtgcagat cgcatcctga agcgcaccag ccacatcact gtggttgtgt
     4141 ccgatcgctg agactctgga gactagcaat gggtcagaaa gtacatccta atggtattcg
     4201 cctgggtatt gtaaaaccat ggaactctac ctggtttgcg aacaccaaag aattcgctga
     4261 caacctggac agcgatttta aagtacgtca gtacctgact aaggaactgg ctaaagcgtc
     4321 cgtatctcgt atcgttatcg agcgtccggc taagagcatc cgtgtaacca ttcacactgc
     4381 tcgcccgggt atcgttatcg gtaaaaaagg tgaagacgta gaaaaactgc gtaaggtcgt
     4441 agcggacatc gctggcgttc ctgcacagat caacatcgcc gaagttcgta agcctgaact
     4501 ggacgcaaaa ctggttgctg acagcatcac ttctcagctg gaacgtcgcg ttatgttccg
     4561 tcgtgctatg aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt
     4621 tgaagttagc ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt accgcgaagg
     4681 tcgcgtaccg ctgcacactc tgcgtgctga catcgactac aacacctctg aagcgcacac
     4741 cacttacggt gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat
     4801 ggctgctgtt gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg
     4861 ccgtaaataa ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc gtaaaatgca
     4921 caaaggccgt aaccgcggtc tggcgcaggg tacggatgtt agcttcggca gcttcggtct
     4981 gaaagctgtt ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat
     5041 gacccgtgca gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg acaaaccgat
     5101 cactgaaaag ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt
     5161 tgccttgatt cagccgggta aagtcctgta tgaaatggac ggtgttccgg aagagctggc
     5221 ccgtgaagca ttcaagctgg cagcagcgaa actgccgatt aaaaccacct ttgtaactaa
     5281 gacggtgatg taatgaaagc aaaagagctg cgtgagaaga gcgttgaaga gctgaacacc
     5341 gagctgctga acctgctgcg tgagcagttc aacctgcgta tgcaggctgc aagtggccag
     5401 ctgcaacagt ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta
     5461 ctgaacgaga aggctggtgc gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg
     5521 ttagcgacaa aatggagaaa tccattgttg ttgctatcga acgttttgtg aaacacccga
     5581 tctacggtaa attcatcaag cgtacgacca aactgcacgt acatgacgag aacaacgaat
     5641 gcggtatcgg tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag actaaatcct
     5701 ggacgctggt tcgcgttgta gagaaagcgg ttctgtaata cagtacactc tctcgatacg
     5761 aataaacggc tcagaaatga gccgtttatt ttttctaccc atatccttga agcggtgtta
     5821 taatgccgcg ccctcgatat ggggattttt aacgacctga ttttcgggtc tcagtagtag
     5881 ttgacattag cggagcacta aaatgatcca agaacagact atgctgaacg tcgccgacaa
     5941 ctccggtgca cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc
     6001 aggcgtaggc gacatcatca agatcaccat caaagaagca attccgcgtg gtaaggtcaa
     6061 aaaaggtgat gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga
     6121 cggttctgtc attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca acagcgagca
     6181 gcctatcggt acgcgtattt ttgggccggt aactcgtgag cttcgtagtg agaagttcat
     6241 gaaaattatc tctctggcac cagaagtact ctaaggagcg aatcatggca gcgaaaatcc
     6301 gtcgtgatga cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta
     6361 agaatgtcct gtcttccggc aaggtcattg ttgaaggtat caacctggtt aagaaacatc
     6421 agaagccggt tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc
     6481 aggtttccaa cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta
     6541 gattcgaaga cggcaaaaaa gtccgtttct tcaagtctaa cagcgaaact atcaagtaat
     6601 ttggagtagt acgatggcga aactgcatga ttactacaaa gacgaagtag ttaaaaaact
     6661 catgactgag tttaactaca attctgtcat gcaagtccct cgggtcgaga agatcaccct
     6721 gaacatgggt gttggtgaag cgatcgctga caaaaaactg ctggataacg cagcagcaga
     6781 cctggcagca atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg
     6841 cttcaaaatc cgtcagggct atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat
     6901 gtgggagttc tttgagcgcc tgatcactat tgctgtacct cgtatccgtg acttccgtgg
     6961 cctgtccgct aagtctttcg acggtcgtgg taactacagc atgggtgtcc gtgagcagat
     7021 catcttccca gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg atattaccat
     7081 taccactact gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct ttgacttccc
     7141 gttccgcaag taaggtaggg ttactaaatg gctaagcaat caatgaaagc acgcgaagta
     7201 aaacgcgtag ctttagctga taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc
     7261 tctgatgtga acgcttccga cgaagatcgt tggaacgctg ttctcaagct gcagactctg
     7321 ccgcgtgatt ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat
     7381 ggtttcctgc ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt
     7441 gaaatcccgg gtctgaaaaa ggctagctgg taattgtcac caattgaatc acgggagtaa
     7501 agacagatga gcatgcaaga tccgatcgcg gatatgctga cccgtatccg taacggtcag
     7561 gccgcgaaca aagctgcggt caccatgcct tcctccaagc tgaaagtggc aatcgccaac
     7621 gtgctgaagg aagaaggttt tattgaagat tttaaagttg aaggcgacac caagcctgaa
     7681 ctggaactta ctctgaagta tttccagggc aaagctgttg tagaaagcat tcagcgtgtc
     7741 agccgcccag gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt tatggcgggt
     7801 ctgggtatcg cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag
     7861 gctggtcttg gtggcgaaat tatctgctac gtagcctaat cggaggaaaa aatgtctcgt
     7921 gttgctaaag caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt
     7981 attacgatca aaggtaaaaa cggcgagctg actcgtactc tcaacgatgc tgttgaagtt
     8041 aaacatgcag ataataccct gaccttcggt ccgcgtgatg gttacgcaga cggttgggca
     8101 caggctggta ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac cgaaggcttc
     8161 actaagaagc tgcagctggt tggtgtaggt taccgtgcag cggttaaagg taatgtgatt
     8221 aacctgtctc tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg tatcactgct
     8281 gaatgtccga ctcagactga aatcgtgctg aaaggcgctg ataagcaggt gatcggccag
     8341 gttgcagcgg atctgcgcgc ctaccgtcgt cctgagcctt ataaaggcaa gggtgttcgt
     8401 tacgccgacg aagtcgtgcg tatcaaagag gctaagaaga agtaaggtaa cactatggat
     8461 aagaaatctg ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc
     8521 gcaactcgcc tggtggtaca tcgtaccccg cgtcacattt acgcacaggt aattgcaccg
     8581 aacggttctg aagttctggt agctgcttct actgtagaaa aagctatcgc tgaacaactg
     8641 aagtacaccg gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct
     8701 ctggaaaaag gcatcaaaga tgtatccttt gaccgttccg ggttccaata tcatggtcgt
     8761 gtccaggcac tggcagatgc tgcccgtgaa gctggccttc agttctaagg tagaggtgta
     8821 agatggctca catcgaaaaa caagctggcg aactgcagga aaagctgatc gcggtaaacc
     8881 gcgtatctaa aaccgttaaa ggtggtcgta ttttctcctt cacagctctg actgtagttg
     8941 gcgatggtaa cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga
     9001 tccagaaagc gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg aataacggca
     9061 ctctgcaaca ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg cagccggctt
     9121 ccgaaggtac cggtatcatc gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg
     9181 ttcataacgt tctggctaaa gcatatggtt ccaccaaccc gatcaacgtg gttcgtgcaa
     9241 ctattgatgg cctggaaaat atgaactctc cagaaatggt cgctgccaag cgtggtaaat
     9301 ccgttgaaga aattctgggg aaataaacca tggcaaagac tattaaaatt actcaaaccc
     9361 gcagtgcaat cggtcgtctg ccgaaacaca aggcaacgct gcttggcctg ggtctgcgtc
     9421 gtattggtca caccgtagag cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg
     9481 tttccttcat ggttaaagtt gaggagtaag agatgcgttt aaatactctg tctccggccg
     9541 aaggctccaa aaaggcgggt aaacgcttgg gtcgtggtat cggttctggc ctcggtaaaa
     9601 ccggtggtcg tggtcacaaa ggtcagaagt ctcgttctgg cggtggcgta cgtcgcggtt
     9661 tcgagggtgg tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc acttctcgta
     9721 aagcagcgat tacagccgaa gttcgtctgt ctgacctggc taaagtagaa ggcggtgttg
     9781 tagacctgaa cacgctgaaa gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag
     9841 tgatcctggc tggcgaagta acgactccgg taactgttcg tggcctgcgt gttactaaag
     9901 gcgctcgtgc tgctatcgaa gctgctggcg gtaaaatcga ggaataagta gcagatggct
     9961 aaacaaccgg gattagattt tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga
    10021 ctgctgtttg ttatcggtgc gctgattgtg ttccgtattg gctcttttat tccgatccct
    10081 ggtattgatg ccgctgtact tgccaaactg cttgagcaac agcgaggcac catcattgag
    10141 atgtttaaca tgttctctgg tggtgctctc agccgtgctt ctatctttgc tctggggatc
    10201 atgccgtata tttcggcgtc gatcattatc cagctgctga cggtggttca cccaacgttg
    10261 gcggaaatta agaaagaagg ggagtctggt cgtcgtaaga tcagccagta cacccgctac
    10321 ggtactctgg tgctggcaat attccagtcg atcggtattg ctaccggtct gccgaatatg
    10381 cctggtatgc aaggcctggt gattaacccg ggctttgcat tctacttcac cgctgttgta
    10441 agtctggtca caggaacgat gttcctgatg tggttgggcg aacagatcac tgaacgaggt
    10501 atcggcaacg gtatttcaat cattatcttc gccggtattg tcgcgggact cccgccagcc
    10561 attgcccata ctatcgagca agcgcgtcaa ggcgacctgc acttcctcgt gttgctgttg
    10621 gttgcagtat tagtatttgc agtgacgttc tttgttgtat ttgttgagcg tggtcaacgc
    10681 cgcattgtgg taaactacgc gaaacgtcag caaggtcgtc gtgtctatgc tgcacagagc
    10741 acacatttac cgctgaaagt gaatatggcg ggggtaatcc cggcaatctt cgcttccagt
    10801 attattctgt tcccggcgac catcgcgtca tggttcgggg gcggtactgg ttggaactgg
    10861 ctgacaacaa tttcgctgta tttgcagcct gggcaaccgc tttatgtgtt actctatgcg
    10921 tctgcaatca tcttcttctg tttcttctac acggcgttgg ttttcaaccc gcgtgaaaca
    10981 gcagataacc tgaagaagtc cggtgcattt gtaccaggaa ttcgtccggg agagcaaacg
    11041 gcgaagtata tcgataaagt aatgacccgc ctgaccctgg ttggtgcgct gtatattacc
    11101 tttatctgcc tgatcccgga gttcatgcgt gatgcaatga aagtaccgtt ctacttcggt
    11161 gggacctcac tgcttatcgt tgttgtcgtg attatggact ttatggctca agtgcaaact
    11221 ctgatgatgt ccagtcagta tgagtctgca ttgaagaagg cgaacctgaa aggctacggc
    11281 cgataattgg tcgcccgaga agttacggag agtaaaaatg aaagttcgtg cttccgtcaa
    11341 gaaattatgc cgtaactgca aaatcgttaa gcgtgatggt gtcatccgtg tgatttgcag
    11401 tgccgagccg aagcataaac agcgccaagg ctgatttttt cgcatatttt tcttgcaaag
    11461 ttgggttgag ctggctagat tagccagcca atcttttgta tgtctgtgcg tttccatttg
    11521 agtatcctga aaacgggctt ttcagcatgg aacgtacata ttaaatagta ggagtgcata
    11581 gtggcccgta tagcaggcat taacattcct gatcataagc atgccgtaat cgcattaact
    11641 tcgatttatg gcgtcggcaa gacccgttct aaagccatcc tggctgcagc gggtatcgct
    11701 gaagatgtta agatcagtga gctgtctgaa ggacaaatcg acacgctgcg tgacgaagtt
    11761 gccaaatttg tcgttgaagg tgatctgcgc cgtgaaatca gcatgagcat caagcgcctg
    11821 atggatcttg gttgctatcg cggcttgcgt catcgtcgtg gtctcccggt tcgcggtcag
    11881 cgtaccaaga ccaacgcacg tacccgtaag ggtccgcgca aaccgatcaa gaaataatcg
    11941 gggtgattga ataatggcaa aggcaccaat tcgtgcacgt aaacgtgtaa gaaaacaagt
    12001 ctctgacggc gtggctcata tccatgcttc tttcaacaac accatcgtga ctatcactga
    12061 tcgtcagggt aacgcgttgg gttgggcaac agccggtggt tccggtttcc gtggttctcg
    12121 caaatccact ccgtttgcag ctcaggttgc agcagagcgt tgcgctgacg ccgtgaaaga
    12181 atacggcatc aagaatctgg aagttatggt taaaggtccg ggtccaggcc gcgaatctac
    12241 tattcgtgct ctgaacgccg caggtttccg catcactaac attactgatg tgactccgat
    12301 ccctcataac ggttgtcgtc cgccgaaaaa acgtcgcgta taacgcctcg ttttccaggt
    12361 ttgttggaga aagaaaatgg caagatattt gggtcctaag ctcaagctga gccgtcgtga
    12421 gggcaccgac ttattcctta agtctggcgt tcgcgcgatc gataccaagt gtaaaattga
    12481 acaagctcct ggccagcacg gtgcgcgtaa accgcgtctg tctgactatg gtgtgcagtt
    12541 gcgtgaaaag caaaaagttc gccgtatcta tggtgtgctg gagcgtcagt tccgtaacta
    12601 ctacaaagaa gcagcacgtc tgaaaggcaa caccggtgaa aacctgttgg ctctgctgga
    12661 aggtcgtctg gacaacgttg tataccgtat gggcttcggt gccactcgtg cagaagcacg
    12721 tcagctggtt agccataaag caattatggt aaacggtcgt gttgttaata tcgcttctta
    12781 tcaggttagt ccgaatgacg ttgtaagcat tcgtgagaaa gcgaagaagc agtctcgcgt
    12841 gaaagccgct ctggagctgg ctgagcagcg tgaaaagcca acttggctgg aagttgatgc
    12901 tggcaagatg gaaggtacgt ttaagcgtaa gccggagcgt tctgatctgt ctgcggacat
    12961 taacgaacac ctgatcgtcg agctttactc caagtaaagc ttagtaccaa agagaggaca
    13021 caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga
    13081 gttcgacgca cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg
    13141 gtaacgcact gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg
    13201 agattgatgg tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg
    13261 aaatcctgct caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc
    13321 ttaccttgaa taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg
    13381 atgtcgaaat cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta
    13441 ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgaattc
    13501 attcggaaga agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg
    13561 tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctggaca
    13621 agctggtcat cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg
    13681 cggcaaccat tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc
    13741 ctgaagtgaa agaagagaaa ccagagttcg atccgatcct gctgcgccct gttgacgatc
    13801 tggaattgac tgtccgctct gctaactgcc ttaaagcaga agctatccac tatatcggtg
    13861 atctggtaca acgtaccgag gttgagctcc ttaaaacgcc taaccttggt aaaaaatctc
    13921 ttactgagat taaagacgtg ctggcttccc gtggactgtc tctgggcatg cgcctggaaa
    13981 actggccacc ggcaagcatc gctgacgagt aaccggatca caggttaagg ttttactgag
    14041 aaggataagg tcatgcgcca tcgtaagagt ggtcgtcaac tgaaccgcaa cagcagccat
    14101 cgccaggcta tgttccgcaa tatggcaggt tcactggttc gtcatgaaat catcaagacg
    14161 actctgccta aagcgaaaga gctgcgccgc gtagttgagc cgctgattac tcttgccaag
    14221 actgatagcg ttgctaatcg tcgtctggca ttcgcccgta ctcgtgataa cgagatcgtg
    14281 gcaaaactgt ttaacgaact gggcccgcgt ttcgcgagcc gtgccggtgg ttacactcgt
    14341 attctgaagt gtggcttccg tgcaggcgac aacgcgccga tggcttacat cgagctggtt
    14401 gatcgttcag agaaagcaga agctgctgca gagtaatctg aagcaacgta aaaaaacccg
    14461 ccccggcggg tttttttata cccgtagcat ccccacttat ctacaatagc tgtactcttt
    14521 ttgttcatcc cctggagtat ttatgtggtt acttgaccag tgggcagagc gtcatatagc
    14581 agaagcgcaa gcgaaaggtg agtttgataa tctggcaggt agcggcgaac cattgatact
    14641 ggatgatgat tctcatgtgc caccggaatt acgtgcgggg tatcgcttgc tgaagaatgc
    14701 tggttgctta ccgccagaac ttgagcagcg gagggaagca attcagcttc tggatatcct
    14761 gagaggtatc cgtcacgatg atccgcaata tcaagaggtt agccgtcgat tgtcattact
    14821 ggaattgaag ctgcgacaag ctggatttag taccgatttt ttacgcggcg attatgctga
    14881 caagttgttg gacaaaatca acgataacta gtggagtatg tatgtatcgc attggtgagc
    14941 tggcaaaaat ggcggaagta acacctgaca cgattcgtta ttacgaaaaa cagcagatga
    15001 tggagcatga agtgcgtact gaaggcgggt ttcgcctgta taccgaaagc gatctccagc
    15061 gattgaaatt tatccgccat gccagacaac taggtttcag tctggagtcg atccgcgagt
    15121 tgctgtcgat ccgcatcgat cctgaacacc atacctgtca ggagtcaaaa ggcattgtgc
    15181 aggaaagatt gcaggaagtc gaagcacgga tagccgagtt gcagagtatg cagcgttcct
    15241 tgcaacgcct taacgatgcc tgttgtggga ccgcccatag cagtgtttat tgttcgattc
    15301 ttgaagctct tgaacaaggg gcgagtggcg ttaagagtgg ctgttgattt tttgcactgg
    15361 caggattaca ctcgcgccgt aaaataacca attggagttt ttatgagccg atatcagcat
    15421 actaaagggc agataaggga taatgcgata gaagcattac tacatgatcc cttattccga
    15481 cagcgcgtag agaaaaataa gaaggggaaa ggcagttaca tgcgaaaagg aaaacatggc
    15541 aatcggggga actgggaggc cagtggcaag aaagtaaatc acttttttac cactggtctt
    15601 ctgctttcag gcgcttgtta agagcggtta ttctgctctt tcagcaaatc acgaatttct
    15661 gccagtaata cttcttcttt agttggtgca ggtgcggcag ctggttcttc ttttttccga
    15721 ttcagtttgt tgattagctt aatcgccata aagatggcaa aggccacaat cagaaaatca
    15781 aagacgtttt gaatgaagac accgtaatgc atcacaacag cagggatatc cccctgcgca
    15841 tcgcgtagcg tgacagcaaa ctgtttaaag tcgatcccgc caattaataa gcccagaggt
    15901 ggcatgatga tatcggcaac cagtgaagat acaatcttcc cgaatgccgc accgataatg
    15961 acacccaccg ccaaatccac cacgttcccg cgcatcgcaa attcgcgaaa ttctttaata
    16021 atgctcatgt tattctccct atgaagccga caaccataag tctaacaaat gttaagccat
    16081 tttcctgcca ggaatgaatt taattaatcc ttgataatca aagggaaata ataaggcgtc
    16141 attagacgcc ttattaatta caagaagaaa gggcttggct ggaagagtct ttcgacgtcg
    16201 gtaataaact ttttatctgt gaggaacata attacgtgat cgccttgctc aatgcgcaga
    16261 ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg ctccaataat cgttcctggc
    16321 ggtagcttga tttcgtcaat gactctgccg acaacgcgtg aggtgctttc atcaccgtga
    16381 gcaacggctt caatagcttc tgctacgccg cggcgcaatg aggaaacacc aacaatatct
    16441 gcttttcgca catggctaag caacgcagaa atagttgctt gttgtggtga aattgcaata
    16501 tcgatcacgc tcccctgaac cagatccaca taagcgcgac gctggatcaa taccatcacc
    16561 tttttcgcac ccatacgttt ggcaagcatg gcggacatga tattggcctc gtcatcgttg
    16621 gtgacggcaa taaacagatc aacttgatcg atatgttctt cggccagcag ttcttgatcc
    16681 gacgcatcac caaaaaagac gatcgtattc tgcaactttt ccgccagttc ggcagcgcgc
    16741 tgctgattac gttcgatgag tttaacgctg taatcttttt ccagacgacg cgccagccct
    16801 gcaccgatat taccgccacc aaccagcatg atccgcttat acggtttttc cagtcgctgt
    16861 aattcactca tcaccgcgcg gatatgctgt gaagcggcaa taaagaacac ttcatcacca
    16921 gcttcaacaa tggtcgaacc ttgtggacga atggggcgat cgtggcggaa aattgctgcc
    16981 acacgagtat cgatatgtgg catatgttcg cgcatggtcg aaagtgcatt accaatcagc
    17041 gggccgccat aataggcttt aactacagcg aggctgactt taccctcagc gaagttcacc
    17101 acctgcaatg cgcctggata ctcaatcagt cggtaaatat tatcgataac caactgctct
    17161 ggtgcgatca gatgatcaat cggtacagca tctgaatgaa atagcttatc ggcatcgcgt
    17221 acgtagtctg gtgagcggat gcgagcgatg cgattagggg tgttgaaaag tgagtaggct
    17281 acctggcagg caaccatatt ggtttcatct gaactggtta cagcaaccag catatcggcg
    17341 tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc catgcccctg cacgactcgc
    17401 aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt tggtatcgac aacagtaata
    17461 tcgttgttct cgccaaccag gttttccgcc agtgtgccgc caacctggcc ggcacccaga
    17521 atgataattt tcatcagtct cgacccgtta tctcatcact ttttgattag cttagcgtaa
    17581 aagaagccgt cgccctcttc ggcaccaggc agattttgtt tacccggttg ctctggtgtt
    17641 cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa aagctttaat ctgcaggcta
    17701 ttctcttccg gtaacaccga acaggtggca tagaccaggg ttccaccgga ttttaaatgc
    17761 ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga gttccgggat atcgcgatcg
    17821 cgacgtaacc atttaatatc cggatggtga cgaatcaccc ccgttgctga acaaggcgca
    17881 tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt gggaagggta acggccatca
    17941 ccttgtttca cggttgcctt cataccaagg cgttttaaat tgtcgtaaac gcgagagagg
    18001 cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg gtgccacctc aaggatatgc
    18061 gttgttttac cgccgggggc ggcacaaaga tccagaatgt gttcaccgtt ttgtggcgca
    18121 agccaggtca tgcaaccttg tgctgatgca tcctgaacgg taacccatcc gtcttcaaaa
    18181 ccaggtagcg catgaacagg tgcaggtgtt tccagacgta cagcatcagg gtaatccgca
    18241 tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa gccagctgtc gcgggaatga
    18301 tgcgtacgat taacgcgcag ccacattggc ggacgctggt tattggcttc gacgatggat
    18361 tgccactgct ctggatacgc tttttgcaga cgcttcagca accaggaagg atgcagataa
    18421 cgtgcatcac tggcattaaa cttggctaat aactcttctt gctgacgctg gaactggcgt
    18481 aatacaccgt taatcaaccc tttaagttgc ggacgcttaa ttgcgatagc gccttcaacc
    18541 gtttcagcca gcgcagcatg aggtggaatg cgggtataaa gcagttgata caaaccaacc
    18601 ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg gacgggccat taacttatta
    18661 atcagccagt ctaactgcga aagcgtacgc agtacgccaa agcacaactc ttgaagaagt
    18721 gctttgtctt tatcagaaac tttttgctgg agcggtggca gaatgttgct taatgattgc
    18781 ccttgctcga cgacttgttc aacggcctgg gccgccatgc tacgtaaatt acgttgtttt
    18841 ttcataagta taaaaatgcc cggtaagacc gggcttagaa gagtggacta tcaggccaga
    18901 cggttgcccg gaacaaacca ttcccgacga gaattcagga ggtcttgcgc gctcatcgct
    18961 ttcttacccg caggttgtaa cgagagcaga ttcaggattc catcaccagt tgcaacctga
    19021 atgccttgtt tgttggcttc aaggatcgtt cctggtgcag cgttggttgc cgtatcaatg
    19081 accgacgctt tccagacttt aaccggctgt ctttcaattt ccagccagct cattggccat
    19141 ggattgaaag cgcgaatgta gcgttcaagc tgtgctgccg aaagtgacca gtcaatacgc
    19201 gcttcttctt tactcaactt ttcggcgtaa gtgacaagag tttcgtcctg aacttctggt
    19261 ttcgccgtgc cgtctgccag ttgtttcaac gtggtgataa gcccttgtgg gccaagctct
    19321 gccagcttgt cgtacagcgt accactggta tcttctgcag taatcgggca ggagagctta
    19381 tagagcatgt caccggtgtc taaaccgaca tccatttgca taatggtcac accagtttct
    19441 gcatcacccg cccatagtga gcgttggatt ggtgcagcac cgcgccagcg tggcagcagt
    19501 gaaccatgaa cgttgataca gccaagacgc ggcatctcca gcactgcttt aggcagaatt
    19561 aagccatagg cgacgacgac cataacatca gcctgcagat cggcgaccag ttgctggttt
    19621 tcttgtggac gcagggaaac aggttgaaaa acgggcagac ctttttcctc agccagaact
    19681 ttaaccgggc tgggcatcag ttttttaccg cgtcctgccg gtcggtctgg ctgggtgaac
    19741 acgccaacga cgttatgacc agaagacaac agcgcgtcga gatgacgcgc tgcaaagtca
    19801 ggtgtacccg caaaaataat acgtagtgat tctgacacgt tagttcttat ccttaagccc
    19861 gggctttcag acgatccagt ttttcaactt tctgacgaat acgttgttgt ttcaacggtg
    19921 acagataatc cataaacagt ttgccgacca ggtgatccat ctcatgctga atacagatgg
    19981 ctaacagacc gtctgcttcc aattcaaatg gtttaccgtc gcggtccagg gcgcgaattt
    20041 taactttctc tgcgcgtggc actaaagcac gttgttcagg gatcgacagg caaccttctt
    20101 caatgcctgt ttcgccgctt ttttctaaaa gctccggatt gattaacacc agccgttcgt
    20161 cacggttttc cgaaacatca ataacaatga tacgttgatg gatatcaacc tgggttgccg
    20221 ccaggccaat accttcttct gcgtacatcg tctcgaacat atcatcgacg atacgctgaa
    20281 tttctgcatt cacttcttct accggtttag caactttgcg aagccgctcg tccggaatat
    20341 gtaacacttg caaaactgac ataaatctcc agagatgtgt tcaggagtta gaaagattat
    20401 ttcttctatt ctagacaaat ccccctctga ttgacagcat cactgaccaa tcgcaaagat
    20461 tgctaaggct gcttatggca gggagataag gatggtcgat acagatattt ggctgcgttt
    20521 aatgagcatc agcagcttgt acggcgatga tatggtccgt atagctcact ggctggcaaa
    20581 acagtcgcat attgatgcgg ttgtattgca gcaaacaggg cttacattgc ggcaggcaca
    20641 acgctttctt tcatttccgc gaaagagtat cgaaagctca ctttgttggt tggagcaacc
    20701 caaccatcat ttaatccctg cggacagcga attttatcct cctcaacttc tggcgacgac
    20761 agattacccc ggcgcactgt ttgttgaagg agaactgtac gcgctgcatt catttcagct
    20821 tgccgtagtg gggagtcggg cgcattcatg gtatggcgag cgatggggac gattattttg
    20881 cgaaactctg gccacgcgtg gagtgacaat tacgagtgga ctggcgcgtg gaatcgatgg
    20941 tgtggcgcat aaagcggcct tacaggtaaa tggcgtcagc attgctgtat tggggaacgg
    21001 acttaatacc attcatccgc gccgccatgc ccgactggct accagtttgc ttgaacatgg
    21061 tggggcactt gtctcggaat ttcccctcga tgtttcaccc cttgcttaca atttcccacg
    21121 aagaaatcgc attatcagtg gtctaagtaa aggtgtactg gtggtggaag cggctttgcg
    21181 cagtggttcg ttggtgacag cacgttgtgc gcttgagcag gggcgtgaag tttttgcctt
    21241 gccaggacca atagggaatc cgggaagcga agggcctcac tggttaataa aacaaggtgc
    21301 gattcttgtg acggaaccgg aagaaattct ggaaaacttg caatttggat tgcactggtt
    21361 gccagacgcc cctgaaaatt cattttattc accagatcag caagacgtgg cattgccatt
    21421 tcctgagctc ctggctaacg taggagatga ggtaacacct gttgacgtcg tcgctgaacg
    21481 tgccggccaa cctgtgccag aggtagttac tcaactactc gaactggagt tagcaggatg
    21541 gatcgcagct gtacccggcg gctatgtccg attgaggagg gcatgccatg ttcgacgtac
    21601 taatgtattt gtttgaaacc tatattcaca cagaagctga gttgcgtgtg gatcaagaca
    21661 aacttgaaca ggatcttacc gacgcaggat ttgatcgaga agatatctac aatgccctgc
    21721 tttggctgga aaagcttgcg gattatcagg aaggactgac agaaccgatg caactggcct
    21781 ctgatccact ctccatgcgt atttataccc ctgaggagtg tgaaagactg gatgccagct
    21841 gtcgtggttt tctgcttttc cttgagcaaa ttcaggtgct taaccttgaa atccgtgaaa
    21901 tggtgataga gcgagtgctc gcgctggata ccgcagagtt cgacctggaa gacctgaaat
    21961 gggtaatcct gatggtgttg ttcaatattc cgggctgcga aaatgcgtac cagcaaatgg
    22021 aagaattact ctttgaagtg aatgaaggta tgctgcatta atcatttctt taattcagca
    22081 taagttgtta tggcgaaatc agcactgttc acggtgcgta ataatgagtc ctgcccaaag
    22141 tgcggggctg aactggttat tcgatccggg aaacacggtc cgtttcttgg atgctcacag
    22201 tatccggcgt gtgactacgt ccgtcctctg aaatcttcag cggatggaca tattgtcaaa
    22261 gttctggagg ggcaggtttg ccctgcatgt ggcgcaaatc tggtattacg ccagggacgc
    22321 tttggtatgt ttattggttg cagtaactac cctgaatgcg aacataccga acttatcgat
    22381 aaaccggacg aaacagcaat tacatgcccc caatgtcgga cgggccatct ggtccagcgc
    22441 cgctcccgtt atggcaaaac atttcactcc tgtgatcgct acccggagtg tcaatttgcc
    22501 attaacttca aacccatagc tggagaatgc cctgagtgtc attatccact actcatcgaa
    22561 aagaaaaccg cgcagggtgt aaaacacttt tgtgccagta aacaatgtgg aaagccggtt
    22621 tcggcggaat aataacgtga ataataacct gcaaggagac gctatcgcag ctgcgataga
    22681 tgtcctcaat gaagaacgtg tcatcgccta tccaacggaa gccgttttcg gtgttgggtg
    22741 cgatcctgat agcgaaacag cagtgatgag actgttggag ttaaaacagc gtccggttga
    22801 taaggggctg attttaatcg cggcaaatta cgagcagctt aaaccctata ttgatgacac
    22861 catgctgact gatgcgcagc gtgaaaccat tttttcccgc tggccaggtc ctgtcacctt
    22921 tgtctttccc gcgcctgcga caacaccgcg ctggttgacg ggccgctttg attcgcttgc
    22981 tgtacgagtc accgaccatc cgttggtggt tgctttgtgc caggcttatg gtaaaccgct
    23041 ggtttctacc agtgccaact tgagtggatt gccaccttgt cgaacagtag acgaagttcg
    23101 cgcacaattt ggcgcggcgt tcccggttgt gcttggtgaa acgggggggc gtttaaatcc
    23161 ttcagaaatc cgcgatgccc tgacgggtga actgtttcga caggggtaac ataatggaaa
    23221 cctatgctgt ttttggtaat ccgatagccc acagcaaatc gccattcatt catcagcaat
    23281 ttgctcagca actgaatatt gaacatccct atgggcgcgt gttggcaccc atcaatgatt
    23341 tcatcaacac actgaacgct ttctttagtg ctggtggtaa aggtgcgaat gtgacggtac
    23401 cttttaaaga agaggctttt gccagagcgg atgagcttac tgaacgggca gcgttggctg
    23461 gtgctgttaa taccctcaag cggttagaag atggacgctt gcagggtgac aataccgatg
    23521 gtataggttt gttaagcgat ctggaacgtc tgtcttttat ccgtccgggt ttacgtattc
    23581 tgcttatcgg cgctggtgga gcatctcgcg gcgtactact accactcctt tccctggact
    23641 gtgcggtgac aatcactaat cggacggtat cccgtgcgga agagttggct aaattgtttg
    23701 cgcacactgg cagtattcag gcgttgggta tggacgaact ggaaggtcat gagtttgatc
    23761 tcattattaa tgcaacatcc agtggcatca gtggtgatat tccggcgatc ccgtcatcgc
    23821 tcattcatct aggcatttat tgctatgaca tgttctatca gaaaggaaaa actccttttc
    23881 tggcatggtg tgagcagcga ggctcaaagc gtaatgctga tggtttagga atgctggtgg
    23941 cacaggcggc tcatgccttt cttctctggc acggtgttct gcctgacgta gaaccagtta
    24001 taaagcaatt gcaggaggaa ttgtccgcgt gaatcaggcc atccagtttc cggacaggga
    24061 agagtgggac gagaataaaa aatgtgtatg ttttcccgct ctcgtgaatg gtatgcaact
    24121 gacatgcgcg atctctggcg agagtctggc gtatcgcttt actggagata cgccagaaca
    24181 gtggttagcg agttttcgtc agcatcgctg ggacctggaa gaagaagcgg aaaacttaat
    24241 tcaggaacaa agtgaagatg atcaaggctg ggtctggtta ccctgatcca gatattcgtc
    24301 cttccatttc acgtaattat tcgcggaata gcgtaaccca gccttctctt catcacttaa
    24361 cgggcggatc tgtttgacgg ggctaccgag atacagatat ccgctctcca gccgtttatt
    24421 ttgtgggacc agactacccg caccaatcat cacatcatct tctactattg cgccatcaag
    24481 taaaattgag cccatcccaa ccaaaactcg attgccaatg gtgcagccgt ggagcatcac
    24541 cttgtgacca acagtgacat cttcgccaat ggttaatggg ttgccatctg ggttgtacga
    24601 ggatttatga gtgacatgca acatactgcc atcctggata ttggtgcgtg ctccgatctg
    24661 tacataatgt acatctccac gaatcacaac gagcggccag atccccacat catcagccag
    24721 acgaacgcca ccaatcacga cactgctatc gtcgatcatt acgcgctgac cgatttgtgg
    24781 aaaaagatcg cggtatgggc gtaaaacatc agacatactt acctcagcaa taaatgattt
    24841 actaatgact ttgggggcat tattggcctt gtgcaagtct tttagtatgc aaaaaagcac
    24901 cgttttgtgt gcgattgcag caaaaagggt gaaaaaacaa caaacagaaa aaaagatcaa
    24961 aaaaatactt gtgcaaaata ttgggatccc tataatgcgc ctccgttaag acgacaacgt
    25021 gaaacacttc acaggatggt cggaacaacg aagagaaaaa atcctgaaat tcagggttga
    25081 ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgagctgaa agccgcgtcg
    25141 c
//
LOCUS       DAJTNN010000044        25001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTNN010000044 DAJTNN010000000
VERSION     DAJTNN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.044"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..799
                     /locus_tag="OO150_001607"
     CDS             <1..799
                     /locus_tag="OO150_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR5515796.1"
                     /translation="LMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRL
                     LMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTF
                     TVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMS
                     WGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFS
                     YWIYGCMGVLAALFMWKFAPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(843..1241)
                     /gene="psiE"
                     /locus_tag="OO150_001608"
     CDS             complement(843..1241)
                     /gene="psiE"
                     /locus_tag="OO150_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5515797.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            1511..1789
                     /locus_tag="OO150_001609"
     CDS             1511..1789
                     /locus_tag="OO150_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515798.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(1836..3932)
                     /locus_tag="OO150_001610"
     CDS             complement(1836..3932)
                     /locus_tag="OO150_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5515799.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIGGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNVGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3932..4669)
                     /locus_tag="OO150_001611"
                     /pseudo
     CDS             complement(3932..4669)
                     /locus_tag="OO150_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            complement(4666..5304)
                     /locus_tag="OO150_001612"
     CDS             complement(4666..5304)
                     /locus_tag="OO150_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5515800.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(5418..5660)
                     /locus_tag="OO150_001613"
     CDS             complement(5418..5660)
                     /locus_tag="OO150_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515801.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(6122..7771)
                     /gene="pgi"
                     /locus_tag="OO150_001614"
     CDS             complement(6122..7771)
                     /gene="pgi"
                     /locus_tag="OO150_001614"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5515802.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            8296..9645
                     /gene="lysC"
                     /locus_tag="OO150_001615"
     CDS             8296..9645
                     /gene="lysC"
                     /locus_tag="OO150_001615"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5515803.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            9897..10169
                     /locus_tag="OO150_001616"
     CDS             9897..10169
                     /locus_tag="OO150_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5515804.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(10302..11174)
                     /gene="rluF"
                     /locus_tag="OO150_001617"
     CDS             complement(10302..11174)
                     /gene="rluF"
                     /locus_tag="OO150_001617"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5515805.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            11522..12469
                     /locus_tag="OO150_001618"
                     /pseudo
     CDS             11522..12469
                     /locus_tag="OO150_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            12539..13345
                     /locus_tag="OO150_001619"
     CDS             12539..13345
                     /locus_tag="OO150_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5515806.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALVWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTHG"
     gene            13355..13762
                     /locus_tag="OO150_001620"
     CDS             13355..13762
                     /locus_tag="OO150_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5515807.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            13762..14256
                     /locus_tag="OO150_001621"
     CDS             13762..14256
                     /locus_tag="OO150_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5515808.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            14322..15119
                     /locus_tag="OO150_001622"
     CDS             14322..15119
                     /locus_tag="OO150_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5515809.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            15130..15972
                     /locus_tag="OO150_001623"
     CDS             15130..15972
                     /locus_tag="OO150_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5515810.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITTALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLSRF
                     FAPA"
     gene            16006..17235
                     /gene="sorE"
                     /locus_tag="OO150_001624"
     CDS             16006..17235
                     /gene="sorE"
                     /locus_tag="OO150_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5515811.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            17618..18307
                     /gene="pepE"
                     /locus_tag="OO150_001625"
     CDS             17618..18307
                     /gene="pepE"
                     /locus_tag="OO150_001625"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5515812.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEDNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(18398..20029)
                     /locus_tag="OO150_001626"
     CDS             complement(18398..20029)
                     /locus_tag="OO150_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5515813.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTAALTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(20249..23937)
                     /gene="metH"
                     /locus_tag="OO150_001627"
                     /pseudo
     CDS             complement(20249..23937)
                     /gene="metH"
                     /locus_tag="OO150_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            24137..24961
                     /gene="iclR"
                     /locus_tag="OO150_001628"
     CDS             24137..24961
                     /gene="iclR"
                     /locus_tag="OO150_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5515814.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         5882 a         6612 c         6531 g         5976 t
ORIGIN      
        1 gctgatgtca cgcggcaagc aagaacaggc ggaaagtatc ctgcgcaaaa ttatgggcaa
       61 cacgcttgca actcaggcag tacaggaaat taaacactcc ctggatcatg gccgcaaaac
      121 cggtggtcgt ctgctgatgt ttggcgtggg cgtgattgtc atcggcgtta tgctctccat
      181 cttccagcaa tttgtcggca tcaatgtggt gctgtactac gcgccggaag tgttcaaaac
      241 gctgggggcc agcacggata tcgcgctgtt gcagaccatt attgtcggag ttatcaacct
      301 caccttcacc gttctggcaa ttatgacggt ggataaattt ggtcgtaagc cactgcaaat
      361 tatcggcgca ctcggaatgg caatcggtat gtttagcctc ggtaccgcgt tttacactca
      421 ggcaccgggt attgtggcgc tactgtcgat gctgttctat gttgctgcct ttgccatgtc
      481 ctggggtccg gtatgctggg tactgctgtc ggaaatcttc ccgaatgcta ttcgtggtaa
      541 ggcgctggca atcgcggtgg cggcccagtg gctggcgaac tacttcgtct cctggacctt
      601 cccgatgatg gacaaaaact cctggctggt ggcccatttc cacaacggtt tctcctactg
      661 gatttacggg tgtatgggcg ttctggcagc actatttatg tggaaatttg ccccggaaac
      721 caaaggtaaa acccttgagg agctggaagc cctctgggaa ccggaaacga agaaaacaca
      781 acaaactgct acgctgtaat cttcctgtcc agcacgccgc gccagttcgg cgtgctgtct
      841 ttttactccc gcttcagccg tttcgaatta cacagccaca gagtgatcac cagtaacagg
      901 atcgcagccg agtagatcaa cacatccagt ggcgatttat gatcgacgat gatcaagcgc
      961 acaatggcgg tgatcccaat atagacaaag taacgtagcg ggaagtgaaa accggactga
     1021 aagtacttca caatcagcgc gataaattcg aaatagagaa agtaaaccac cagctcatat
     1081 ttgctggttt gttctggcgc gaacagcaca tctgccagat gcaccgtttc tttgccgagg
     1141 aagacaacca aaatcaggcc gaggcacagc aggccaagat tgagtacggt ctgcaaaatg
     1201 gtggagataa actccacgcg cggacgagag agtgacgtca tagcagtacc tccttcacag
     1261 tggcgagctt cctgtataac gcaaaaatgt gacggagatc tatatttttt gtgtatgttt
     1321 tgttcaacct ggatttggag cgcgcctcca ccaaagccgg atatacctac ccgtaggccg
     1381 gataagccgc gcctggcggc gcatccggca agacaaaccg cacccaagcc ggtaattcaa
     1441 aaatcaccca ttcaggtgat tcccagccac cagcaatgcg cgaaaaatcg acaatgttcc
     1501 tctggaacaa atgaaacgta acctgataaa ggtggtaaaa atgaagcctt attttgctgc
     1561 tttgatgtta tcagtctctg ttcttcctgc ttatgctggt ccgctcggta ctgctgataa
     1621 agccgaccta ccgcaaagca atgtctccag cccaatgatg gcccagtcgc tcaggcaacc
     1681 agacctgcaa ccgatatcga cggacaggaa gacagaatgt tttcgccttt acacaccaga
     1741 tcgcaaaccg ggggtgaact gcgttcctga tggctcgaca ggccattaag aatgaaggtg
     1801 ccagtgagtg cgaaaactgg cacctttccc atgacttagc ggaaattgac gctacggtcg
     1861 ctggtcatgt catacaactg gaacttacgc ccaagttgct gaccaccgtc acgcgtcagc
     1921 ggcgtccagc caatcgccgc acggctgcgt gtcgggccag acgagaagag atccaacggt
     1981 accgagacat acacgccttt ggtgaagtcc ccttcgccgt actcctcttt caaaacgttg
     2041 gtgatcgtgg catagccacc caccaccacg ccgctgtcaa agcgtttggc gatctccagc
     2101 gtgccgcctt tatcccctgc cagatactgc ccgacgctgg ctttcaccaa cacgtcctgg
     2161 gcgaaagatg gcgtccagta ggcggtcaga tgtccggttt tcacgctgta gtcggtgaat
     2221 ttcatcatat cttttgcact acgccagtcg cgctgtttaa cgtagttggc atccagacca
     2281 aacgcccagt tactgtccag cggacgatac aacacttcgg cccccgcacc gccaaacatg
     2341 gtttcgagat aacctccata gacctgaccg tagaagccgt tgcccagatg ctggaagtag
     2401 ttggcttgca ggttattcac atagacatcg ttctgcacat actcgcgcac atgggtacga
     2461 acgcgcggta agtgcgagtc ctgcggcgga ttggtgtagt taaacttgtc gtagttgttg
     2521 gcaagatttg caaacaggct gccggtggtc agcagatggt ccgtcagcca caaatctgcc
     2581 attcccatca cgcccagctg atacatgtaa aagttttccg ggccaccgac cgactggttc
     2641 agcaccggat cgatatggaa atcaaagcgt gatttgtcga tataccagcc ctgctcggtg
     2701 gactgcggaa ccactggctc gacgcgtttt tgcgccagct tcgtttcgtg gcccaacggc
     2761 tctccggcga gatgattttt caggctggcg acatcggttt ccgtcgtcac ctgcggcatg
     2821 ttaagacgat tttccgtaat gcggatcgtt ttgatcccat ccggcagatc gttcatcacg
     2881 atccgattag cacggatgat cccttcgcgc gaatcacgat atttcacctg ctcgccggtg
     2941 acatacagcg tatcgccttt cgcctggatc tgcggatcgg caagtccgac attgtatttc
     3001 aacagcgtta actgattcgc caccaccgaa tgctgcaaaa tggcatcctg cggctgcggt
     3061 tgatattgcg ggcgggcgtt atcgttgtaa gacgggcgca gatcgttaaa gttggtgcgc
     3121 agcgtgacgc caaacataaa ggtgttgcca cgttcatagc taaggttaac gtcggcccaa
     3181 tcggtaacgc gataaatcgc accgacgtta aacttacttt tttgctccag cttcccggca
     3241 aaatcctgct gataattatt gccttcatac tccagtttca ggcgcagcgg ttgccagggc
     3301 gtctggtatt ccacgccgcc aaacagtgag gcaggaccgt ggaacatctg gctaccgccg
     3361 atagatcccg cctgtttgta gctgttatcg cgatagcaat atttatcact ggctgaacag
     3421 agcggatttt tcacattacc gctggtgccc agataacccc agcccaggcc gagcgtaaaa
     3481 tcgaacggcc cccaggcttt gctggcaaca agatattccg catcaaacag ccccgtaccg
     3541 ccgatatccc tcgcaccaac cgccacttgc ggcagccagt aactctcttc ccacaaacgc
     3601 agtttgagat cgaaggcttt atctttatac gtttgatcgc cagagaacgc ttcgacgctg
     3661 ctgtactgcc gggtgcgcac gtcggtgtag cgcagcgttg tttccagcca cgggaagagt
     3721 tgcactgaag ctgagtaata acggtactga tcgttatcgc gatagttcag gctcaactcc
     3781 ccttcccgcg ccatgcgtgc ggtgggcgtt tgtaataatc ctacgccacc gaaatccgac
     3841 tgcgacgggc caatgggcgc aggatatgtt tcgccgtagc aagccatgct aatgcccagc
     3901 gccagtaagc taagcagatg tctttttttc attattgggg tatccgctgc gtcagagtct
     3961 gaagaatgtc ggcgttaagg gcatcaggcg tctcactcca gacggagtcc gcgaggccaa
     4021 cataaataat gctgccgggc atcggctcta cgtgacgctt gttccagtaa gccaccggcg
     4081 ctttttgcgt gcgtccgtcc gggtaaacca cccacgcgta gctgcgatcc gcaccgctaa
     4141 gcaggttctg cccggagaga tagctcgcca cgtcgcgacc tggagtgaat ggctgcttgc
     4201 caggacggct gataagcccg aacaacgtga ccgtggacgg cggtggtccg acccacagcg
     4261 tgtagttgcc ctgcaacggt gggttaccgc gttcggcaac gcgcacgata tcgggatcaa
     4321 gattgatttt ttgcctgccc gtcactttca acgcctgaat ttgctggcgt aaggcgttaa
     4381 tggcggcggc atcgtcggcg ctggagtctg cggcctgttc agccagccgt gtcagcaacg
     4441 cctgctgctg acgcaatgct gccgccgttg ccagctcttc gctaatcacc gcaccgggcc
     4501 accagttgtt tgccagccgc ggttagccta ccagatcgat taaatgctct gcgcctgtta
     4561 gtgttttggc ctcactgctg ccattgctga acaccttgac ggttcctgcc gcgaagaccg
     4621 atgacgctcc cacactcaaa agcaacgcga caaaagtttg tttaatcatt gttttgccgc
     4681 cttgatgaga gtggttttca ccgggaaata atccgcgccg agatactgtt ccgactggcg
     4741 aatttgccct tcgctgtcga tccagtagcg gttatgccag cgcgcctggt cggtggagac
     4801 ttcttcgtcc agcacgcgaa ccaaggtttc atcgctgccg actttgacgg tatcggtgcc
     4861 atcccatttg aagactgagc gtgcggtggc gtagcgtacc tgctggtact cggtccagcc
     4921 catcgtgcgc gtccatgttg caccatcaac aatttgtgcg ggtttaatca gcgggtcggc
     4981 ggcaaggtta ttcacttcaa tcaggttgtc gccgccaagc aaagtcttca ccagacgacc
     5041 atgttgtgtg acaagagtgg cttgatcctg agtgacccat ttttgttgtc cgtcttcagc
     5101 gaaggccagc accacaaaca attgcggccc gccattaagc tgcatgtact ggctggcgta
     5161 gggcatattt tgaatatcat cgtccgtcag ctgtacgcct ggcgtgccga acagactgtc
     5221 ccacagtgaa ttgcccagct ctttagtcgt ggctgaacag gcctgtaata gcaggcagat
     5281 aagaatgagt gcaggtcgct tcacgactct tctccagagg ggcagaataa ccacaccgga
     5341 gtgtggttat ggtaaatccc ttcatacttc aagccacaga tgcaatgact caaattattt
     5401 tggggataca tactttatta ctgggtactg gtggtcgtgg ttgtggtggt cccggtatta
     5461 gaaccatcac cgccaccggt tgccgcgagc gcgacaccca ccgccgagct tacggtgctg
     5521 acgctggtcg cggaggaact acccgccgaa accgacgttg ctgccgaccc tgccgcttcg
     5581 cccacctgta caggtgcagc ccacgcagaa gtcgccgcaa gcgcagatat ggcaaaaatg
     5641 ccatacagaa cttttttcat aacaatttcc cttcattgaa tgaatggaga tttacccaaa
     5701 aacatttcgg gcgggatcga gtatacacaa ctaaagcatg cgaattacgg cgcggggaat
     5761 tagcgatggc gttttaggat aattggaatg aatatcaaat ataagcttca acttatattt
     5821 aaataacaat aaaaatcata aacttaaaat aaaaacaaaa gtaattaatc tgcgtattct
     5881 ccgaaataaa caacgattaa aaatctattt taggattaac ctcacggtat gatttccgtt
     5941 aaatcacaga caaggggatt tatctgataa aacaatgccg acttattgtc ggcattgttt
     6001 ttaaggttat ttaactcctc atactatgca ttaatgtcga tattggcatc aggctccggt
     6061 tgccggatgc ggcgtaaacg ccttatccgg cctacatatc ggcatcaggc acgcatgatg
     6121 attaaccgcg ccacgcttta tagcggttaa tcagaccatt ggtcgagcta tcgtggctgc
     6181 tgatttcttt gtcatctttc agctctggca gaatacggtt cgccagttgt ttacccaact
     6241 ccacgcccca ctggtcgaag gtaaagatgt tcaggatcac gccctgagta aagattttgt
     6301 gctcatacag cgcaatcaac gcccccaggc tgaacggagt gatttcgcgc agcaggatgg
     6361 agttggtcgg gcggttacct tcgaacactt tgaacggcac cacgtagtca agcgttgccg
     6421 gatctttacc ctgatcgcga tattcctgct caaccacttc gcgggattta ccaaacgcca
     6481 gcgcttcggt ctgggcgaag aagttagaca gcagtttctg gtggtgatca gagagcgggt
     6541 tatgggtgat agccggggcg atgaaatcgc acggtaccat tttggttccc tggtggatca
     6601 gctggtagaa cgcgtgctga ccgttagtgc ctggttcacc ccagataatc gggccagtct
     6661 ggtaatccac aacgttaccg ttacggtcaa catacttacc gttggactcc atattgccct
     6721 gctggaagta cgccgcgaaa cggtgcatat actggtcata cggcagaatc gcttcagttt
     6781 ccgcaccaaa gaaattgttg taccagatgc caatcagcgc cagcaggaca ggcaggtttt
     6841 tctcggcagg cgtggtggag aaatgcttgt ccatcgcgtg tgcgccagaa agcagttcaa
     6901 cgaagttatc aaagccgatg gagagaacaa tcgacaggcc aatcgctgac cacaaagagt
     6961 aacggccgcc gacccagtcc cagaactcga acatgttggc agtatcaata ccaaactcgc
     7021 caacggcttt ggcattggtg gaaagcgccg caaagtgttt tgcaacgtgt ttttcatcac
     7081 ctgccgcttt caggaaccag tcacgcgcgc tatgggcgtt ggtcatagtt tcctgagtgg
     7141 tgaaggtttt agatgctacc aagaacagcg tggtttccgg gtttactttt ttcagcactt
     7201 cagcgatgtg agtcccatcg acgttagaaa caaagtgcat gttcaggtga tttttgtacg
     7261 gacgcagtgc ttcggtcacc atgtacgggc cgaggtcaga accaccgatc ccgatgttca
     7321 ctacgtcagt gattgctttg ccggtataac ccttccactc accggagata atcgcttccg
     7381 agaaggtttt catcttctcc agcaccgcgt tgacttccgg cattacgtct ttgccatcaa
     7441 ccagaatcgg ggtattgcta cggttacgca gcgctacgtg cagcacggcg cggttttcag
     7501 tgcggttgat cttctcgcca gagaacatcg acttaatcgc gcccgccaga tcgcactctt
     7561 tcgccagatc ctgtaatttc gccagcgtct cttcagtgat gcggtttttg gagtaatcca
     7621 ccagcatctg atcgtcgaat gttgcggaga acttagaaaa acgatcgccg tctttagcaa
     7681 aaagatcggc gatcgtaacg tctttcattt catcgaagtg tttctgtagt gcctgccagg
     7741 cagcggtctg cgttggattg atgtttttca ttagcaatac tcttctgatt ttgagaattg
     7801 tgactttgga agattgtagc gccagtcaca gaaaaatgtg atggttttag tgccgttagc
     7861 gtaatgttga gtgtaaaccc ttagcgcagt gaagcattta ttagctgaac tactgaccgc
     7921 caggagtgga tgaaaaatcc gcatgacccc gtcgttgaca accgccccgc tcacccttta
     7981 tttataaatg tactacctgc gctagcgcag gccagaagag gcgcgttgcc caagtaacgg
     8041 tgttggagga gccagtcctg tgataacacc tgagggggtg catcgccgag gtgattgaac
     8101 ggctggccac gttcatcatc ggctacaggg gctgaatccc ctgggttgtc accagaagcg
     8161 ttcgcagtcg ggcgtttcgc aagtggtgga gcacttctgg gtgaaaatag tagcgaagta
     8221 tcgctctgcg cccacccgtc ttccgctctt cccttgtgcc aaggctgaaa atggatcccc
     8281 tgacacgagg tagttatgtc tgaaattgtt gtctccaaat ttggcggtac cagcgtagct
     8341 gattttgacg ccatgaaccg cagcgctgat attgtgcttt ctgatgccaa cgtgcgttta
     8401 gtagtcctct cggcttctgc tggtatcact aatctgctgg tcgctttagc ggaagggctg
     8461 gaacctggcg agcgattcga aaaactcgac gctattcgca acatccagtt tgccattctg
     8521 gaacgtctgc gttacccgaa cgttatccgt gaagagattg aacgtctgct ggagaacatt
     8581 actgttctgg cagaagcggc ggcgctggca acgtctccgg cgctgacaga tgagctggtc
     8641 agccacggcg agctgatgtc gaccctgctg tttgtcgaaa tcctgcgcga acgcgatgtt
     8701 caggcacagt ggtttgatgt acgtaaagtg atgcgtacca acgaccgatt tggtcgtgca
     8761 gagccagatg tagccgcgct ggcggaactg gccgcgctgc agctgctccc acgcctcaat
     8821 gacggcttag tgatcaccca gggatttatc ggtagcgaaa ataaaggtcg tacaacgacg
     8881 cttggccgtg gaggcagcga ttatacggca gccttgctgg cggaggcttt acacgcatct
     8941 cgtgttgata tctggaccga cgtcccgggc atctacacca ccgatccacg cgtagtttcc
     9001 gcagcaaaac gcattgatga aatcgcgttt gccgaagcgg cagagatggc aacttttggt
     9061 gcaaaagtac tgcatccggc aacgttgcta cccgcagtac gcagcgatat cccggtcttt
     9121 gtcggctcca gcaaagaccc acgcgcaggt ggtacgctgg tgtgcaataa aactgaaaat
     9181 cctccgctgt tccgcgcgct ggcgcttcgt cgcaatcaga ctctgctcac tttgcacagc
     9241 ctgaatatgc tgcattctcg cggcttcctc gcggaagttt tcggcatcct cgcgcggcat
     9301 aatatttcgg tagacttaat caccacgtca gaagtgagcg tggcattaac ccttgatacc
     9361 accggttcaa cctccactgg cgatacgttg ctgacgcaat ctttgctgat ggagctttcc
     9421 gcactgtgcc gggtggaagt agaagaaggt ctggcgctgg tcgcgttgat tggcaatgac
     9481 ctgtcaaaag cctgcggcgt tggcaaagag gtattcggcg tactggaacc gttcaacatt
     9541 cgcatgattt gttacggcgc atccagccat aacctgtgct tcctggtgcc cggcgaagat
     9601 gccgagcagg tggtgcaaaa actgcatagt aatttgtttg agtaaatact gtatggcctg
     9661 gaagctatat ttcgggccgt attgattttc ttgtcactat gcttatcaat aaacgagcct
     9721 gtactctgtt aaccaacgtc tttatcgaga ataattgcct ttaatttttt tatctgcatc
     9781 tctaattaat tatcgaaaga gataaatagt taagagaagg caaaatgaat attatcagtt
     9841 ctgctcgcaa acgaattccc gtacactgaa ctctctggct cactgagggc accatcatgg
     9901 cactcccccg tattacccaa aaagagatga ctgaacgcga gcagcgtgaa cttaaaacac
     9961 tgctggatcg ggcgcggatt gcgcatggtc gcgtactgac caactccgaa accaacagca
    10021 ttaagaaaga gtacatcgat aaattaatgg tcgagcgtga ggctgaagcg aaaaaagccc
    10081 gccaattgaa gaaaaagcag gcttataaac cggatcctga agcgtcattt agctggtcag
    10141 caaatacgtc gacgcgcggc aggcgctaat gaacaatcaa cgcctgagat tttcactcgt
    10201 ctgtgtggac acaatcttcc cctcacccta accctctccc cgaaggggcg aggggactgt
    10261 ccgagctctt tttggacgtt ttcatcagtc tgaggcgtta atcagcgtcc cttcttttta
    10321 cgccccggcg aggtaaaacg cttaccgttg gacgccgggc gaccgccttt ttccgccgtt
    10381 ttttccatct taacgactgg acgtttgatg cccgctgttt tcggtttggc cttcgcttta
    10441 ggttttgcct cggaagagga attttcgatg agcttaaaga ggtcgattaa ctcatcgtcg
    10501 gttaaatcgc gccactcccc cagcggaatg ccgcttaagc tgacgttcat aatgcgcgta
    10561 cgttccagct ttttcacttc atagccgaaa tgctcgcaca tgcgccggat ctgacggttc
    10621 agcccctgca ccagggtaat gcggaagaca aacggcgctt cttttttgac tttgcacttt
    10681 ttcgtcaccg tcccgaggat cggcaccccc gcccccatac cacgaataaa ttcatcggta
    10741 atcggtttat cgaccgtcac cagatactct ttctcatgat cgttacccgc acggaggatc
    10801 ttattcacca gatcgccgtg attggtgagg aaaatcagcc cctgggagtc tttatccagg
    10861 cggccaatcg ggaacacgcg tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg
    10921 ccatcttcgg tggtgctgac aataccgacg ggcttgttca gggcgataag taccaaatct
    10981 tcggcttccc gaggctcaat caactgaccg tttactttca caatgtcgcc gggtttcacc
    11041 tgatcgccaa tggtggctcg cttgccatta aggaacacat tgccttgctc gatatagcga
    11101 tccgcttcgc ggcgtgagca aattccgctt tcgctgatgt atttatttaa acggactgat
    11161 gagtcgggca gcataaattc tcctgtaaaa cgcggaatat accgtacctc ttagcgggga
    11221 aaaacagggt tatgcggcaa cgtcgaaatt aaatgtgatc tgccacgcct tttatcacaa
    11281 aagtgtctcc ggaagtacga tgcaaatttg cctggcaaaa taaggataat caacatatta
    11341 cgaatcgctg cacacctgtg ctgatgacta ctacgcagag aaacaaatgt gcaagagaaa
    11401 gcgctatatc tccccatgca aagagtgaaa aatcgctttg aaaggccctc atgatggggt
    11461 tatatcagat caagcggtga gaaagcatat ttgcacaaat gtgcagaacc aggaggcggc
    11521 aatggaaaac agtgacgata tccgtttgat tgtgaagatt gcccaactct attacgaaca
    11581 ggatatgacg caggcgcaaa tcgcgcgcga actgggtatt taccgcacca ccatcagccg
    11641 cttgcttaaa cgaggccgcg atcagggaat tgtcaccatc gccatcaact atgactacaa
    11701 cgaaaatctc tggctggagc agcaactgaa gcaaaagttt ggcctgaaag acgttgtggt
    11761 ggtgtcggga aatgatgagg atgaagagac tcaactggcg atgatggggt tacacggcgc
    11821 gcaactgctg gatcgcttgc tggagcctgg cgatattgtc ggtttttcct ggggtcgcgc
    11881 ggtgagcgca ctggttgaaa acttgccgca ggcggggcaa tcgcggcagt taatctgcgt
    11941 accaattatt ggcggaccgt ccggtaaact cgaaagccgc tatcatgtaa acacattaac
    12001 ctacagcgcg gcagcgaagc tgaaagggga atcgcatctc gcggattttc cggctctgct
    12061 ggataaccca ttaattcgta atgggatcat gcagtctcag cactttaaaa ccatctctgc
    12121 ctactgagat aatctggatg tcgccctggt gggaattggc tcaccggcca ttcgcgacgg
    12181 cgctaactgg catgcgtttt atggtggtga agagagtgac gacctgaatg cccgccaggt
    12241 tgctgacgat atttgctcgc gcttttttga tattcacggc gcaatggttg aaacgaatat
    12301 gagcgaaaaa acactctcta tcgaaatgaa taaattaaag caggcacgat attccattgg
    12361 cattgccatg agtgaagaaa aatacagcgg aattgttggt gcactgcgtg gaaaatatat
    12421 taattgtctg ataacgaata gcagcacagc tgaactatta ctgaaataaa aatcttcccg
    12481 atgtttagcg taataaaaat tacggcgggg ccctattatc taaaatcagg agtctgttat
    12541 gcaaacgtgg ttaaatttgc aggataaaat cattattgtc accggcggcg catccggtat
    12601 tggtctggcg attgtagagg aattattagc acaaggcgca aacgtacaga tggtagatat
    12661 tcacggtggc gatggtcaat atgaaggtca taaaggctat cagttttggc cgaccgatat
    12721 ttccagcgcc aaagaggtaa atcatacggt agcggaaatt atccagcgtt ttggtcgcat
    12781 cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt ctgctggtcg atgagaaagc
    12841 gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca atatcaacca
    12901 gaaaggcgtt tttctgatgt cgcaggcggt ggcgcgacag atggtcaaac aacatgatgg
    12961 cgtgattgtg aatgtttcct cagaaagtgg actggaaggc tcagaaggcc aaagctgtta
    13021 cgccgctacc aaagccgcgc ttaatagctt cacgcgatcc tggtcgaaag aattgggtaa
    13081 acacggtatc cgtgtggtcg gtatcgcgcc ggggattctg gaaaaaacag gactgcgcac
    13141 gccggaatat gaagaagcgc tggtgtggac gcgcaatatc accgtcgagc agctgcgtga
    13201 aggctatacc aaaaacgcca ttcctattgg gcgcgccgga agattagcag aagtcgctga
    13261 ttttgtttgt tatctgctgt ctgaacgcgc cagctatatc accggagtaa ccactaacat
    13321 tgcgggcggc aaaacgcacg gctaaggagg ctttatggtc aatgccattt tttgcgccca
    13381 cggcaaatta gcttgcgcca tgctggaatc ggttcagatg gtgtacggcg acgccagggt
    13441 cgaggcagtt gaatttgtgc ccggcgagaa cgccggagac atcgtagcaa agctggaaaa
    13501 gttagtaagc attcacaatc aagatgagtg gctgattgcc gtggatttac agtgcggcag
    13561 cccgtggaat gcggcagcga tgcttgcgat gcgcaatcct cgtctgcggg taatcagcgg
    13621 cctttctctg ccgctggcgc tcgaactggt tgataaccag gacagcatga atgttgatga
    13681 actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc gttgtctgga aacaactggc
    13741 aacggcagag gaggatttct gatgaacatt acgctcgccc gcattgatga ccgcctgatc
    13801 cacggtcagg tcactaccgt ctggtcgaaa gtggctaacg cccagcgcat tattatctgc
    13861 aatgacgaag tttataacga tgaagttcgc cggacattat tgcgtcaggc tgcgccaccg
    13921 ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg ctgtttatca caacccgcaa
    13981 tatcaggacg aaaccgtttt ttatcttttc actcgcccac aggatgcctt agcgatggtt
    14041 cgccaagggg taaaaataga caccctaaat attggcggca tggcctggcg gcccggtaaa
    14101 aaacagttaa ccaaagcggt ttcattagat gatgatgata ttaacgcatt tcatgagtta
    14161 aataatcttg gcgtcatact ggatttacgc gtcgtagcgt cagatccctc aatcaatatt
    14221 atcgacaaaa taaacgaaca gttaatcgca aattaaataa tagcgcttgt gtacatatgc
    14281 tttaacaggc agggatgacg acacttaaaa ggtgaaaaat catggaaata agtaccctac
    14341 aaatcattgc tatatttctt ttttcctgta ttgccggaat gggcagcgtg ctggatgaat
    14401 ttcagactca tcgcccgtta attgcctgta cggtgattgg tttaattctc ggtgatttaa
    14461 aaaccggaat aatactcggt ggtacgctgg aattgatagc tctcggctgg atgaacgtcg
    14521 gcgcggcgca atctccggat tctgcactcg ccagcataat ctccgccatt ctggttatcg
    14581 ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc
    14641 aggtgctgac tgtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag
    14701 cagcggaaga agcacggttt cgcactctcg atattctgca tgtctccgcg tttggcgtgc
    14761 aggcgctgcg cgttgctatt ccggcactga ttgtctcact gttcgtcagc gccgatatgg
    14821 tgagcaatat gctgagcgcg ataccggaat tcgtgacgcg cggtctgcaa attgctggcg
    14881 gttttatcgt ggtggtcggt tacgccatgg tgctacgtat gatgggcgtg aaatatttga
    14941 tgcctttctt tttcctcggt ttcctcgcag gtggctacct cgatctcagt ctgctggcgt
    15001 tcggtggcgt cggcgtgatc atggccctgg tctacatcca gctaaatcca cagtggcgta
    15061 aagctgaacc tcaactccag gccaccgcct ccaccgccct tgaccaactt gacgattaac
    15121 ggagccatca tggaacagag aaaaattaca cgcagcgatc tggtgagcat gtttctgcgc
    15181 tccaacctgc aacaggcgtc ctttaacttt gaacgtattc acgggctggg tttttgctac
    15241 gacatgatcc ccgccatcaa acgactttac ccattaaaag aggatcaggt tgcggcgctc
    15301 aggcgacacc tggtgttctt caataccacg ccagccgtat gtggcccggt catcggcgtc
    15361 actgccgcca tggaagaggc gcgggccaac ggcgcggaaa ttgatgacgg taccattaac
    15421 ggcatcaaag tcggtctgat gggaccattg gcaggagttg gcgatccact ggtctgggga
    15481 acgctgcgcc cgattaccac cgcgctcggc gcatctctgg cactttcggg caacattctc
    15541 ggcccgctgc tgttcttctt tattttcaat gcggtgcgtc tggcgatgaa gtggtatggc
    15601 ctacagctcg gctttcgcaa aggggtgaat atcgtcagcg atatgggcgg gaatgtgctg
    15661 caaaaactca ccgaaggcgc gtcgattctc ggactgtttg tgatgggcgt gctggtcacc
    15721 aaatggacgt caatcaacgt accgttggtg gtttcacaaa cgcatgccgc cgatggctcc
    15781 accgtcacca tgaccgtgca gaacattctc gaccaacttt gccctggttt gctggcgctc
    15841 ggtctgacgc tgcttatggt tcgtctgctc aacaaaaaaa ttaacccggt atggctgatt
    15901 ttcgccctgt ttggcttagg gattatcggt aatgcgctgg gcttcctgtc cagattcttc
    15961 gccccggcat gactgccggg gccatcgctc aacatgaggt ggtttatgaa aacaacagct
    16021 ctgcgtcttt atggtaaacg tgatttacgc ctggaaacct ttgaccttcc tgaaatgcag
    16081 gaggatgaaa tcctcgcgac ggtggtcact gacagcctgt gcctctcttc ctggaaagag
    16141 gccaatctgg gtgaaaacca taaaaaagta cccgacgatg tggcgaccaa ccccatcatc
    16201 atcggccacg agttttgcgg cgatattctg gccgtgggta aaaagtggca gcacaaattc
    16261 cagccgggtc agcgttatgt gattcaggcc aacctgcaac tccccgaccg cccggactgc
    16321 cccggctact ccttcccgtg ggtgggcggc gaagccacgc atgtggttat tcccaacgag
    16381 gtcatggaac aagattgcct gctggcatac gacggcgaaa cctattttga aggctcactg
    16441 gttgaaccgc tttcctgcgt gattggcgcg ttcaacgcca actatcatct tcaggaaggt
    16501 agttataacc acacgatggg gattcgcccg caagggcgca tgctgatcct cggcggcacc
    16561 ggaccaatgg gactgttggc gattgattat gcgctacatg gacccgttaa cccgtcgcag
    16621 ctcgtcatta ccgataccga caacgataaa ttgagttatg cgcgcaagca ctatccatca
    16681 gaaccgcaaa cactgattca ttatctcaat gccgccgatg cagcatttga tacgctaatg
    16741 gcgctgagtg gcggtcacgg cttcgatgat attttcgtct ttgtgcctaa tgaaggactg
    16801 gtgactctcg cctcttcctt gctggcgaca gatggttgcc tgaatttctt cgccggaccg
    16861 caggataaac atttcagcgc gccaattaat ttctacgatg tgcattatgc atttacccac
    16921 tacgtgggca cgtcaggcgg caataccgac gacatgcgcg cggcggtcaa actgattgaa
    16981 gagaaaaaag tgcaggccgc aaaagtggta acacatattc ttgggctgaa tgccgcgggc
    17041 gaaaccacgc ttgaattgcc tgccgtcggc ggcggcaaaa agctggtgta taccgggaaa
    17101 tacctgccgc tgacgtcact cacgcagatt caggatcaag cactggcggc gattctggcg
    17161 cgtcatcagg ggatctggtc cggtgaggcg gagcaatatc tgctcactca tgcagaggca
    17221 atttcccatg attaatcgcg ctacttaact ctgcatgtcg ctggctggtc gccgcggcaa
    17281 cttcgcgacc tgatgtgcgg ctgattgctg aagcggccca atgtgcacga gaaggataaa
    17341 aaaacatcag cgccttaagg ggcgctcaaa ttggtggcaa agtacacatt gtttatgccg
    17401 gatgcggaat aaagccttat tcggcctaca taatctcgaa aattcaatat attgcagtta
    17461 tctggtaggc ctggtaagcg tagcgcatca ggcaatgctg tgtttgtcat cagtctcagc
    17521 tcccggtgca atacgtgaca tcgtgagcca tgtcagtaag tggttaaatt cgcagtggta
    17581 gcatgccttc cgtgatttct tactaaccgg agaagtaatg gaactgcttt tattgagtaa
    17641 ctcgacgctg ccgggtaaag cctggctgga acatgcactg ccgctaattg ctgaacagtt
    17701 gcagggtcgc cgctcagcgg tgtttatccc tttcgctggc gtaacgcaga cctgggatga
    17761 ttacacagcg aaaacggctg cggttctcgc tccgctgggt gtttctgtca ccggtattca
    17821 tagcgttgtc gatcccgttg ccgcgattga aaatgctgag atcgtgattg tcggcggcgg
    17881 gaatactttc cagttgctga aacagtgccg cgagcgcggg ctgctggcac caattactga
    17941 cgtggttaaa cgtggtgcgc tgtatattgg ctggagcgca ggcgctaacc ttgcttgccc
    18001 aaccattcgt accaccaacg atatgccgat tgtcgatccg caaggtttcg atgcgctaaa
    18061 tctgttcccg ttgcaaatca acccgcactt caccaacgcg ctgccggaag gccataaagg
    18121 tgaaacccgt gagcagcgta ttcgcgaact gctggtcgtc gcgccagaac tgacgattat
    18181 tggtctaccg gaagataact ggatcacagt gagtaaaggt cacgctacgc tgggtggccc
    18241 gaacaccacc tatgtgttta aggccggtga agaagcggtt ccgctggaag ctggtcaccg
    18301 tttttaagtg aattgccgga tgcatgatcc ggcatcttgt tcatagtgcc tgatgcgacg
    18361 cttgacgcgt cttatcaggc ctacaggatt cacaatatta atactcgtcc cgtccttcat
    18421 cttcatccga ctgttccagc acactgtaag ccaccgaaca aaacagcgag ttcaggcgct
    18481 gcatatctcc cagtaagcct aaatgtagcg aactggtttc aatgctctgc acgttttgct
    18541 gatgcaggcg atcgacgtgg gcgtgggaat agcggcgatt aagaatgcga aagcgatgct
    18601 tactacggcg caaacgacga gcgctggtga catcgccaga gaagaacacc gacattgcca
    18661 gctttaaatt actgagcaat tgctcataga gcgcatccag ttccttcaac ccatcaagcg
    18721 aaaatgcccg ccgtgctgcc agtgatttgt cagcaatttc gctgcccatg cgctcgacga
    18781 tatcggaggc ctgttcaagg ttgagcgaca tttcaataat ctccgcccag cggcgcgact
    18841 cttcctccgc cagctcttct tttggcatcc gcgccagata cagcttaatg gcggtgtaga
    18901 gaacgttgat atcatctgcc agcttacgca gctctttctc ctgccgtggc tcaccgtgca
    18961 tcactttatt caacccttcc atcatctgtt ccatggcgtc gccaatgcgc agggtttcgc
    19021 gcgcggcgtt cgccagagca agcgtgggcg tatccagcgc gctgacatcc agatgtttag
    19081 gccgtagctg ggtatccagt tccggttcat cgcgaataat cgttttgcaa aaccgtgcca
    19141 tcgggtcaac aaatggcagc atgaccaggc aacgtacaag gttgtagaag acgtggaaat
    19201 agatcaccag ttccgctttt ggcaatgaca gcttccccat tgtctctgcc agcaaatgga
    19261 caaacggcag gataatcagg ctacccacca gcttaaacag cagactaccc agcgcgacac
    19321 ggcgggctgc ggcattggcg gcactgttgt tgagcatcgc cagcaggccg gaaccgaggt
    19381 tagcaccaat caccagacag agcgccacgg ggaaggagat aatgcctgcg gcggtcagag
    19441 ccgcggtcag cagtacagca gcaaggctgg agtagctgat aatggcgaac atcgcgccaa
    19501 tcagcgcatc cagcagaata tcgccggtca gcgaggcaaa gatcacctga acgccgtttg
    19561 cctgggtgat cggcgttacg gcctgcacaa tcaactccag cgccagcaaa atcagcccaa
    19621 gaccaatacc gacgcggccc agttgcccgg cgcgtgactg tttgcgtccg aggaaaaaaa
    19681 tcacgccgat aaaaataagt aacggtgaca gccaggataa gtcgaaggtg agaatacgcg
    19741 ccattagcgc cgtcccgaca tctgccccca gcacaatgac cagagccggt gcgagggcta
    19801 ccagatcctg agcgacgaac gaggtcacca gcatggtggt ggcattactg ctctgtacca
    19861 gtgcggtaac gccgatcccc gcgcaaaagg cgagcggctt tttttcgacg ctccggctaa
    19921 ggacagtacg caaacgcgcg ccgaagacgc gcattacgcc ggttcgaaca atatgagtcc
    19981 cccagaccag cagggcgacg gcagaaagca ggtgaagcag cgttaacacg agttcaggtt
    20041 ctccttatcg ttatatgttc ctgaagcaat tgaatgacca aaggaccgga aacacgctga
    20101 ctggcatctt tactgtgcct tttgacattc gtttttccgg ctaagattat tccagcctga
    20161 ttccagtata agtgtttcat attaataaag agacagggca ctcattgagc gccctgtttg
    20221 ttgtaaggaa aagtgacaga tttgtgaatc agtccgcgtc ataccccaga ttcggtgcca
    20281 gccagcgctc aacatctgag acgctcatac ctttacggcg ggcatagtct tcaacctgat
    20341 cgcgctgaat ttgcgccacg gcgtagtact tgctgtccgg gtggctgaaa taccaacccg
    20401 aaaccgatgc accgggccac atggcgaaag attctgtgag tttcatgccg gtatgttttt
    20461 ccacttccag cagctcccag atggtggctt tttccgtatg ttccgggcag gccgggtagc
    20521 ccggtgccgg acggatgccc tggtagtttt cgcggatcag ttcttcgttg ctgaggttct
    20581 cgttcggcgc atagccccag tagactttac gcacacgctc atggagatac tccgcaaagg
    20641 cttcggctaa acggtcggca agcgctttca ccatgatttt gttgtaatca tcgtgctgcg
    20701 cttcaaaggc atcagccagt gcgtcctctt ccagcccgcc agtcacggca aatgcgccga
    20761 tgtaatctgc tttaccagaa agcttcggcg caacgaagtc agcgagacag tagttagcga
    20821 atcccgtttt ttcggtctgt tgacgcagat ggtggctgac attgatcaca tgggtgcgcg
    20881 tttcgtcacg gtagatttca atgtcatcgc ccacacggtt tgccgggaac aggcccacca
    20941 cgccacgcca cgcggattca gcgttttctc ggcgcttaat ttatccagca tgtcgttggc
    21001 gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa tgcgcggata
    21061 cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgcaat tacgcagagt
    21121 ttcgatgctg gcttcaactt cctgcacgcc gagacggtgc gccaccggcg gcgtgtaagc
    21181 ctgccagtca aaagcgaagt cgttatcgcg cgccgcttcc agcgtgaccg gtggtgtgcg
    21241 cggtttcttg cgcccgtgct gaatacgtac ggtttcgtac tccttgcggg tacgagcgac
    21301 aaaatcatcg cgctgagtat cggaaagcag cgccgccacc acaccaacgg tgcgcgaggc
    21361 attctgcaca tacaccgtcg ggccgctgta gttctgctca attttcaccg ccgtgtgcgc
    21421 ttttgaggtg gtcgcgccac cgatcagtaa cggaatggtg aagccctgac gctccatctc
    21481 tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa ggccaatcaa
    21541 atcagcattc acttctttag cagtacggag aattttttcc gcaggcacca tcacgccgag
    21601 atcgacaatt tcgtagttgt tacattgcag caccacgcca acgatatttt taccgatatc
    21661 gtggacgtcg cccttcacgg tggcgatcac catcttgccg ttggttttgc cctgctcttt
    21721 gctggcttca ataaacggtt cgagatacgc caccgcctgt ttcatgacgc gcgccgattt
    21781 gaccacctgt ggcaggaaca ttttgccttc gccaaacaga tcgccgacca cgttcatgcc
    21841 gtccatcaac gggccttcaa tcacttcaat cgggcgcgta gcctgctggc gggcttcttc
    21901 ggtatcctgc tcgataaact cggtaatgcc tttgaccagc gagtattcca gacgtttatt
    21961 cacttcccac gagcgccact ccgcctgctg ggcgttatcg gtgtcgtcgg ttttgctgcc
    22021 gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcgtcacgac gattaagaat
    22081 cacatcttcc accgcgtcgc gcagttcagc gggtaggtcg tcgtaaatcg ccagttgccc
    22141 cgcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga acactgcgtg
    22201 aatggcttcg cgcaccggat cgttgccacg gaatgagaaa gaaacgttag atacgccgcc
    22261 ggaaatcagc gcgtgcggca gttcgcgttt gatgtcttca cacgcgccga taaagtcctg
    22321 cgcgtagttg ttgtgctctt caataccggt ggcgaccgcg aagatgttcg ggtcgaagat
    22381 gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc gacggcaaat
    22441 ctcgattttc cgtgcgcgag tatcggcctg tccctgttca tcaaaggcca tcaccaccac
    22501 tgccgcaccg tagcgacgca acaatttcgc gtgatggata aaggcgtcga cgccctcttt
    22561 catcgagatt gagttaacaa tgcctttgcc ctgaatacac ttcagccctt tttcgataac
    22621 gtcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt caccggcaat
    22681 cagattgaga aaacgcacca tcgccgcttc ggcatcgagc atcccttcat ccatgttgat
    22741 atcgataatc tgcgcgccgt tttccacctg ctgacgcgcc acatcctgcg cctcgctgta
    22801 tttctcttct ttgatcaggc gcttgaactt agcagagccg gtgacgttgg tgcgttcacc
    22861 cacgttcaca aacaggctat cgtcgccaat gttcagcggc tccaggccag acaaacggca
    22921 ggctacggga atttccggca gtttgcgcgg cgctaatcct tctactgcac gactcatcgc
    22981 tgcaatatgt tgcggcgtgg tcccgcagca accaccgacg atattgagaa agcccgcttg
    23041 cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtatt caccaaaggc
    23101 gttaggcagc ccggcgttcg ggtgggcggt gacgtagcat tccgcaatcc gtgatagttc
    23161 ctgcacgtac tggcgcagtt catcgggccc cagcgcacag ttcaggccaa aggttagcgc
    23221 ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga gcgtgcgccc
    23281 ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca gcgcttcaaa
    23341 ctccgttttc accgcaaaaa ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat
    23401 caggatcaga tccgcaccac cttccaccag cgctttggtg gattcgcgat aggccgccac
    23461 cagctggtca aaagtgatgt tgcggaatgc cggatcgttg acgtccggag aaatagacgc
    23521 cgtgcggttg gttgggccga gaacaccggc aacgtagcgc ggtttctccg gcgtgcgcgc
    23581 ggtccactcg tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga tttccgccga
    23641 cagggattcc atctggtaat ccgccatcgc aatggtcgtg gagttgaagg tgttggtttc
    23701 gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca cttccggttt
    23761 gctgagcacc agcaggtcgt tgttgccttt gagatcgcat ggccagtcgg caaagcgttc
    23821 accacgaaaa tcggcttcgt tcagtcgata actctggatc atggtgccca taccgccgtc
    23881 cagcaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc tgctcacact
    23941 tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac tggcataaac
    24001 gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt aactcatcgg
    24061 atcagttcag caactattgc attagctaac aataaaaatg aaaatgattc ccacgataca
    24121 gaaaaaggag actgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt
    24181 tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga aattactgga
    24241 gtggattgcc gaatccaatg gcagtgtggc actcacggaa ctggcgcaac aagccgggtt
    24301 acccaattcc acgacccacc gcctgctaac cacaatgcaa cagcagggtt tcgtgcgtca
    24361 ggttggcgaa ctgggacact gggcaatcgg cgcacatgcc tttatggtcg gcagcagctt
    24421 tctccagagt cgtaatttgt tagcaattgt tcaccctatc ctgcgcaatc taatggaaga
    24481 gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag cgattattat
    24541 cgaccaggta cagtgtacgc atctgatgcg aatgtccgcg cctatcggcg gtaaattgcc
    24601 gatgcacgct tccggtgcag gtaaagcctt tttagcccaa ctgagcgaag aacaggtgac
    24661 gaagctgctg caccgcaaag ggttacatgc ctatacccac gcaacgctgg tgtctcctgt
    24721 gcatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg acgatgagga
    24781 acatgcgctg gggctacgtt gccttgcagc gtgtattttc gatgagcacc gtgaaccgtt
    24841 tgccgcaatt tctatttccg gaccgatttc acgtattacc gatgaccgcg tgaccgagtt
    24901 tggcgcaatg gtgattaaag cggcgaagga agtgacactg gcgtacggtg gaatgcgctg
    24961 actttttctg gcgggcagag gcaatattct gcccatcata c
//
LOCUS       DAJTNN010000045        24792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTNN010000045 DAJTNN010000000
VERSION     DAJTNN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.045"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..643
                     /gene="yaaA"
                     /locus_tag="OO150_001629"
     CDS             <1..643
                     /gene="yaaA"
                     /locus_tag="OO150_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5515815.1"
                     /translation="ISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYT
                     GLQAETFSEDDFDFAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFW
                     GDIITNKLNEALAAQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIIS
                     FYAKKARGLMSRFIIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(796..1092)
                     /locus_tag="OO150_001630"
     CDS             complement(796..1092)
                     /locus_tag="OO150_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5515816.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGNRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1306..2592)
                     /gene="thrC"
                     /locus_tag="OO150_001631"
     CDS             complement(1306..2592)
                     /gene="thrC"
                     /locus_tag="OO150_001631"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5515817.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(2593..3525)
                     /gene="thrB"
                     /locus_tag="OO150_001632"
     CDS             complement(2593..3525)
                     /gene="thrB"
                     /locus_tag="OO150_001632"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5515818.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(3527..5989)
                     /gene="thrA"
                     /locus_tag="OO150_001633"
     CDS             complement(3527..5989)
                     /gene="thrA"
                     /locus_tag="OO150_001633"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5515819.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6014..6133)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6069..6134)
                     /gene="thrL"
                     /locus_tag="OO150_001634"
     CDS             complement(6069..6134)
                     /gene="thrL"
                     /locus_tag="OO150_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5515820.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(6348..7034)
                     /locus_tag="OO150_001635"
     CDS             complement(6348..7034)
                     /locus_tag="OO150_001635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5515821.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(7434..7574)
                     /locus_tag="OO150_001636"
     CDS             complement(7434..7574)
                     /locus_tag="OO150_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515822.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            7670..8386
                     /gene="arcA"
                     /locus_tag="OO150_001637"
     CDS             7670..8386
                     /gene="arcA"
                     /locus_tag="OO150_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5515823.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(8446..9798)
                     /gene="creD"
                     /locus_tag="OO150_001638"
     CDS             complement(8446..9798)
                     /gene="creD"
                     /locus_tag="OO150_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5515824.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMPLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDWATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9856..11280)
                     /gene="creC"
                     /locus_tag="OO150_001639"
     CDS             complement(9856..11280)
                     /gene="creC"
                     /locus_tag="OO150_001639"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5515825.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(11280..11969)
                     /gene="creB"
                     /locus_tag="OO150_001640"
     CDS             complement(11280..11969)
                     /gene="creB"
                     /locus_tag="OO150_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5515826.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11982..12455)
                     /gene="creA"
                     /locus_tag="OO150_001641"
     CDS             complement(11982..12455)
                     /gene="creA"
                     /locus_tag="OO150_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5515827.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            12666..13535
                     /gene="robA"
                     /locus_tag="OO150_001642"
     CDS             12666..13535
                     /gene="robA"
                     /locus_tag="OO150_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5515828.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(13532..14179)
                     /gene="gpmB"
                     /locus_tag="OO150_001643"
     CDS             complement(13532..14179)
                     /gene="gpmB"
                     /locus_tag="OO150_001643"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5515829.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14231..14752
                     /gene="yjjX"
                     /locus_tag="OO150_001644"
     CDS             14231..14752
                     /gene="yjjX"
                     /locus_tag="OO150_001644"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR5515830.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVTVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14837..15163)
                     /gene="trpR"
                     /locus_tag="OO150_001645"
     CDS             complement(14837..15163)
                     /gene="trpR"
                     /locus_tag="OO150_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5515831.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(15253..17190)
                     /gene="sltY"
                     /locus_tag="OO150_001646"
     CDS             complement(15253..17190)
                     /gene="sltY"
                     /locus_tag="OO150_001646"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5515832.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            17401..19068
                     /gene="ettA"
                     /locus_tag="OO150_001647"
     CDS             17401..19068
                     /gene="ettA"
                     /locus_tag="OO150_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5515833.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(19375..20607)
                     /gene="nadR"
                     /locus_tag="OO150_001648"
     CDS             complement(19375..20607)
                     /gene="nadR"
                     /locus_tag="OO150_001648"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5515834.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(20628..22010)
                     /gene="radA"
                     /locus_tag="OO150_001649"
     CDS             complement(20628..22010)
                     /gene="radA"
                     /locus_tag="OO150_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5515835.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22059..23027)
                     /gene="serB"
                     /locus_tag="OO150_001650"
     CDS             complement(22059..23027)
                     /gene="serB"
                     /locus_tag="OO150_001650"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5515836.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23133..23777
                     /locus_tag="OO150_001651"
     CDS             23133..23777
                     /locus_tag="OO150_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5515837.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23805..>24792
                     /gene="lplA"
                     /locus_tag="OO150_001652"
     CDS             23805..>24792
                     /gene="lplA"
                     /locus_tag="OO150_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR5515838.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELREL"
BASE COUNT         5939 a         6866 c         6165 g         5822 t
ORIGIN      
        1 gattagcacg ctgatgcgca tcagcgacaa actggcgggt atcaacgccg ctcgctttca
       61 tgactggcag ccagatttca cgccggcgaa tgcccgccag gcgattctgg cgtttaaagg
      121 tgatgtctac accggcttgc aggccgaaac cttcagcgaa gacgatttcg attttgccca
      181 acagcatttg cgaatgcttt ccggcttgta tggcgtactg cgcccgctcg atttaatgca
      241 gccttatcgt ctggaaatgg ggatccgtct tgagaatgcc cgagggaaag atctgtatca
      301 attctgggga gatatcatca ccaacaagct gaacgaggcg ctcgcagcac aaggcgataa
      361 tgtggtgatt aatctggcgt cagatgagta ttttaaatcg gtgaagccga agaaattgaa
      421 tgccgagatt atcaagccgg tgttcctcga tgagaagaac ggcaaattta agatcatcag
      481 cttctacgct aagaaagcac gcggtctgat gagtcgtttc attattgaaa atcggctgac
      541 caaaccagag caactgactg gttttaatag cgaaggttac ttctttgatg aagcttcctc
      601 cagcaatggc gaactggtgt ttaaacgcta cgagcagcgt taagtttgca gtcaatgccg
      661 gatgcggcgt gaacgcctta tccggcctac aaaagcatgc agattcaata tattgcagaa
      721 ttcacgtagg cctgataaga cgcgccagcg tcgcatcagg cgctgaatgc cggattgtta
      781 cccggcattt gtcatttagc ggtgatgttt gcctggacca tgaccgccgt gatgatcatg
      841 aggagctttc ttatggtggc gcggcggtgg cggcggtccg tgtgggtgcc agcgattgcc
      901 tcgccattca taatgttgtt tccaccagcc gtggtcgcgc cagtgacctc catcccagta
      961 atagccacga ttatcacgat tgcctatctg taattttact gacgggacta acgtaatttc
     1021 cgcagcctgt gctgccatgg gagcgaccag aaccagggaa agtgcgagta cgatagattg
     1081 catctttttc acaggttact ccttttatca ttgcccgtta tgggtcgata tccggagatt
     1141 acgcgatgag aacgacggtt gttattctcc gcaatcctaa tctctaagtt accgcattta
     1201 ttgagaatat ctcctttttc cctgtcgttt ttctccataa tttcttcata aaaaagccgg
     1261 gctgcataaa agcaaacccg gcctgattga gataatgaat agattttact gatgattcat
     1321 catcaattta cgcaacgcag caaaatcggc gggcagatta tgtgaaagca agggtaaatc
     1381 agcacgttct gccagctctt ttggcagatc caacgtttca ccgagaatcg cttccacgct
     1441 ctctttaaat ttcgccggat gcgcggtgcc gaggaacaag ccatattcgc ctggattcaa
     1501 ctggtcacgc agcgcacgat acgctacggc agcgtgcggc tccgaggtgt agcccagttc
     1561 ttttaactca cgcattgtct gttgcgtggt ttcatcatcc acggctgcat aacccagctc
     1621 tttcagttgc cagattttgc ggcggaacaa ctcttccaca cgcggccagt tgttcggctg
     1681 actcacgtcc atcgcgttgg ataacgtcgc ctgagtcgct ttgggtgacc actgaccgtc
     1741 gtgcaggaaa cgtggcacgg tatcgttcac gttggtcgca gcaataaaac gtttcaccgg
     1801 cagaccgagt gacttcgcca gcagacccgc cgtcaaatcg ccgaagtttc cgcttggcac
     1861 cgagacaacc agctggttgc gcgcttcctg cggcagctgc gcaactgctt caaagtagta
     1921 gcaaatctgc gccagcaaac ggctgatgtt aatcgagtta gctgagttta accccagcgc
     1981 cactttcagc tcttcatcat caaacgcctg cttcaccagc gcctgacagg catcgaaatc
     2041 gccgtcgatg gcaacagttt cgatattgcc gcccaatgta cagaacagtt tttcttgcag
     2101 tggactgatt ttgcctcgtg gatagaggat aaccactttc acattcggta aaccgtagaa
     2161 agcatgagcc actgccgctc cggtatcacc ggaggtcgcg gtcagaatgg tcactggctt
     2221 atcgcccgca atatgggtca gcatttgtgc cataaagcga ccgccgaaat ctttaaatgc
     2281 cagcgttggc ccgtggaaca attccagaca accgacatcg ctttcaacat tggcgaccgg
     2341 agccgggaag gcaaacgccg cgcgcacgcg ctcttccagg atttcctgcg ggatttcatc
     2401 accaataaac gccgagagga tcttcgcact gcgggtgaca aaatccagct tcagcatctc
     2461 atcaatttca gtcaggctga attccggcag gtcgtgcgga aaaaacagcc cctgattttt
     2521 gcccaacccc tgggttacgg cttgcgcaaa gctgacctgc tcattgtgat ctttcagatt
     2581 gtagagtttc atttagtttt ccagtactcg tgcgcccgcc gtatccagcc ggcaaatatg
     2641 aacaaaacct tcctgatttt gcaggtagtt cttacccaac cagtcggcaa cgcgctgggc
     2701 ggtatccggc ttgtcacaca gagcgaacaa ggtcgggccg gagccggaga taccgctcgc
     2761 taccgcgccg atttccgcaa ccgcctgccg cgcctgccgg aagccaggca gtaaccgttc
     2821 acggtagggt tcagcgataa catctttcat cagcttcgcg gcaagctcag gctgacggga
     2881 atagcaggcg tgaatgaagc cagccagatg tcgcccgtgc gcaatgcaat cctggcggcg
     2941 atactgcgcc ggtaaaatag ccctggcttc tgccgttgag actttaatcc ccggatacgc
     3001 cagcacccac agccactcat caaaccctgg cacttgctgg ctgatgatgt cgttttcttc
     3061 gatcatcaac tgcataccac caagaaaaca cggtgccacg ttgtcgtaat gaatgctgcc
     3121 ggagattcgt ccttccagct cgcccatcaa agccagcaaa cgagtgtcat taagcggctt
     3181 gccacagtgt tcattcatcg ccatcagcgc cgcgaccacc gaacaggcgc tggagcctaa
     3241 gcccgaaccg attggcatat tcttttccag agtcatcgcc actggaattt gcttgccaag
     3301 ctcctggcaa aaacgctccc agcactgata aacgatattt tcccgtggtt ctgacggcag
     3361 cttatcggca aagcgtccga ggttgttgag actgaatgtc tctgccgcct caaccgtgac
     3421 tacatctccg agcaatgcac catcaacagg tgtcaccgcc gccccgagca catcaaaccc
     3481 gacgctcata ttggcactgg aagccggggc ataaacttta accatgtcag actcctaact
     3541 tccatgagag ggtacgtagc agatcggcaa agacaccggc agctgtaacg tcattgcccg
     3601 caccatatcc gcgcagcacc aacggcagcg gctgataata gtggctataa aaggccaggg
     3661 cgttttcgcc atttttcact ttgaacagcg gatcattacc atccacttcg gcaatcttca
     3721 cgcggcaggc accatcttca tcaatattgc caacatagcg caaaactttt ccttcatcac
     3781 gggccttcgc cacgcgcgcg gcaaagagat cgtcgagctg tgacagattc gccataaaag
     3841 cggcaacatc accctcagcg ttaaactctg cgggcagcac aggttcaatt tcaatatccg
     3901 ccagctccag ttcacgtccc gtttcacgag cgagaatcaa tagcttacgc gctacatcca
     3961 taccagaaag atcatctcgc ggatccggtt cggtataacc catttcccgc gccagagtag
     4021 tcgcctcgga gaaactcatg ccttcgtcta acttgccgaa gatataagaa agcgaacctg
     4081 aaagaatgcc ggagaacttc atcaattcat caccagcatt gagcagattt tgcaggttct
     4141 caataaccgg taatccagcc ccaacgttgg tgtcatagag gaatttacgc cgcgattttt
     4201 ccgccgcatg acgcaacaga tggtagtaat ccatcgacga ggtgttggcc tttttgttcg
     4261 gcgtgacaac gtggaaacct tcgcgcaaga agtcggcata ttgatccgcc actgcctggc
     4321 tggaggtgca gtcaacaatg accgggttca gcagatgata ttctttcacg aggcgaatta
     4381 agcgcccgag attaaacggc tctttggctt gcgccagttc ttcctgccag ttttccagat
     4441 ttaggccatg cacattggtg agcagtgcct tcgagttggc aacaccgcag acacgtaagt
     4501 cgatatgttt attcttcagc cagctttgtt gacgcttcag ttgctccagc agcgcaccgc
     4561 caacgccacc gacgccaatc acaaacactt cgataacctg atcggtattg aacagcatct
     4621 gatgagtaac gcgcacgcca gtggtcgcat catcgttatt taccacgaca gagattgagc
     4681 gttcagaaga tccctgagca atggcgacaa tgttgatatt ggcgcgggcc agcgcggcaa
     4741 agaatttcgc cgagatccca cgcaaggtgc gcataccatc acctaccacc gagataatgg
     4801 ccagccgttc cgtcaccgcc agcggctcca gtaagccttc tttcagttcc aggtagaact
     4861 cttcctgcat tgcccgttca gctcgcacac agtcgctttg tggaacgcag aaactgatac
     4921 tgtattcgga agatgattgc gtaatcagca ccacggaaat acgggcgcgt gacattgcag
     4981 caaagacgcg cgccgccatg ccgaccattc ctttcatccc cggaccagaa acgctgaaca
     5041 ttgccatatt attcagattg gaaatgcctt tgaccggtaa ttcgtcttca tcacggctgg
     5101 caccaatgag cgtacctggt gcttgaggat ttccggtatt tttaatcagg caagggatct
     5161 ggaactgggc gatgggggta atggtgcggg ggtgaagaac tttagcgccg aagtaggaaa
     5221 gctccatcgc ttcctggtag gacatcgact tcaacaacct cgcatcgggc acctgacgcg
     5281 ggtcgcaggt atagaccccg tcaacgtccg tccaaatctc gcaacaatcg gcgcgtaaac
     5341 aggcagccag caccgccgcg gagtagtcgg aaccgttacg tccaagtacc accagttcgc
     5401 ctttttcatt accggaggtg aaacctgcca tcaacaccat gtgatcagcc ggaatgcggc
     5461 ttgccgcaat acggcgggtg gactcagcaa tatcgacggt agattcgagg taatgcccca
     5521 ctgccagcag tttttcgacc ggatcgataa cggtaacgtt gtgaccacga gcttctaaca
     5581 cgccggccat aatggcgatc gacattttct cgccacggca aatcagcgca gcgttgatgc
     5641 tatccgggca ctgccccaac aaactaatgc catgcaggac atgttttatt tgggcaaatt
     5701 cctgatcgac gaaagttttc aattgcgcca gcgggaaccc cggctgggcg gcggcgagtc
     5761 ccgtcaaaag ttcggcaaaa atacgttcgg catcgctgat attgggtaaa gcatcctggc
     5821 cgctaatggt tttttcaatc atcgccacca ggtggttggt gattttggcg ggggcagaga
     5881 ggacggtggc cacctgcccc tgcctggcat tgctttccag aatatcggca acacgcagaa
     5941 aacgttctgc atttgccact gatgtaccgc cgaacttcaa cactcgcatg gttgttacct
     6001 cgttaccttt ggtcgaaaaa aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc
     6061 tgtacgcgtc agcccgcacc gttacctgtg gtaatggtga tggtggtggt aatggtggtg
     6121 ctaatgcgtt tcatggatgt tgtgtactct gtaattttta tctgtctgtg cgctatgcct
     6181 atattggtta aagtatttag tgacctaagt caataaaatt ttaatttact cacggcaggt
     6241 aaccagttca gaaactgcta tcagacactc tttttttaat ccacacagag acatattgcc
     6301 cgttgcagtc agaatgaaaa gctgaaaaat acttactaag gcgtttttta tttggtgata
     6361 tttttttcaa tatcatgcag caaacggtgc aacattgccg tgtctcgttg ctctaaaagc
     6421 cccaggcgtt gttgtaacca gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc
     6481 agcaatgcca tgactcgttc gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca
     6541 cttttcgtcg gttgttgtat taatgttgct aattgatagc aatagaccat caccgcctgc
     6601 cccagattga gcgaaggata atccgccacc atcggcacac cagtaagaac gtcagccaac
     6661 gctaactctt cgttagtcag cccggaatct tcgcgaccaa acaccagcgc ggcatggctc
     6721 atccatgaag atttttcctc taacagcagc accagttcta ctggcgtggc gtagtaatga
     6781 tatttcgccc gactgcgcgc agtggtggcg acagtgaaat cgacatcgtg taacgattca
     6841 accagtgtcg ggaaaacttt aatattatca ataatatcac cagatccatg tgcgacccag
     6901 cgagtggctg gctccagatg tgcctgacta tcgacaatcc gcagttcgct aaaacccatc
     6961 gttttcattg cccgcgccgc tgccccaata ttttctgctc tggcgggtgc gaccagaata
     7021 atcgttatac gcatattgcc actcttcttg accaaatgac cgcgaacagg gtgatcactg
     7081 tcaacttatt acacggtgcg aatttacaaa tccttaacgt aagtcgcaga aaaagccctt
     7141 tacttatctt aaaaaaggct aaactatttc ctgactgtac taacggttga attgttaaaa
     7201 aatgctacat atccttctgt ttacttagga taattttata aaaaataaat ctcgacaatt
     7261 ggattcacca cgtttattag ttgtatgatg caactagttg gattattaaa ataatgtgac
     7321 gaaagctagc atttagatac gatgatttca tcaaactgtt aacgtgctac aattgaactt
     7381 gatatatgtc aacgaagcgt agttttattg ggtgtccggc ccctcttagc ctgttatgtt
     7441 gctgttaaaa tggttaggat gacagccgtt tttgacactg tcgggtcctg agggaaagta
     7501 cccacgacca agctaatgat gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta
     7561 cgtactttag tcatgttacg ccgatcatgt taatttgcag catgcatcag gcaggtcagg
     7621 gacttttgta cttcctgttt cgatttagtt ggcaatttag gtagcaaaca tgcagacccc
     7681 gcacattctt atcgttgaag acgagttggt aacacgcaac acgttgaaaa gtattttcga
     7741 agcggaaggc tatgatgttt tcgaagcgac agatggcgcg gaaatgcatc agatcctctc
     7801 tgaatatgac atcaacctgg tgatcatgga tatcaatctg ccgggtaaga acggtcttct
     7861 gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg atgttcctga ctggccgtga
     7921 caacgaagtc gataaaattc tcggcctcga aatcggtgca gatgactaca tcaccaaacc
     7981 gttcaacccg cgtgaactga cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct
     8041 gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac aagttcaatg gttgggaact
     8101 ggacatcaac agccgttcgt tgatcggccc tgatggcgag cagtacaagc tgccgcgcag
     8161 cgagttccgc gccatgcttc acttctgtga aaacccaggc aaaattcagt ctcgtgctga
     8221 actgctgaag aaaatgaccg gccgtgagct gaaaccacac gaccgtactg tagacgtgac
     8281 gatccgccgt attcgtaaac atttcgaatc tacgccggat acgccggaaa tcatcgccac
     8341 catccacggt gaaggttatc gcttctgtgg tgatctggaa gattaatcgg ctttaccacc
     8401 gtcaaaaaaa acggcgcttt ttagcgccgt ttttattttt caaccttatt tccagatacg
     8461 taattgatcg tccattgtaa cttctttact ggctttcatt ttcggcagtg aaaacgcata
     8521 ccagtcgata ttacgagtca caaacatcat gccggccagc gccaccacca gcacactggt
     8581 tcccaacaac agcgcgctat cggcagagtt gagcagtccc cacatcacac catccagcaa
     8641 caacagcgcg agggtaaaca acatgctgtt gcgccagcct ttcaataccg cctgcaaata
     8701 aataccgttc attaacgccc caatcagact ggcgattatc catgccacgg taaaaccgat
     8761 atgttcagaa agcgccagca agagcaaata aaacatcacc aatgaaagcc ccaccagcaa
     8821 atattgcatt gggtgtaaac gttgcgcggt gagcgtttca aaaacaaaga acgccataaa
     8881 agtcagtgca atcagcagaa tggcgtactt agtcgcccag tccgttaatt ggtattgatc
     8941 ggctggcgtc gtcactgcga cgctaaacgc ggggaagttt tcccagccgg tatcattgcc
     9001 tgaagcaaaa cgctcaccga gattattagc aaaccagctg ctttgccatt gcgcctgaaa
     9061 acctgactcg ctaacttccc gtttggctgg tagaaaatca cctaaaaaac tgggatgcgg
     9121 ccagttgctg gttaagggca tttcgctatt acgcccggca ggcaccacag aaagatcgcc
     9181 ggtaccgctt aaattcagtg ccatattcag ctgcaggttc tgcttccgcc agtccccttc
     9241 gggtaaaggg atatgcacgc cctgcccgcc ttgctctaac ccggtaccgg gttcaatggt
     9301 cagtgccgtt ccgttaactt caggcgcttt caccacacca ataccacgcg catccccgac
     9361 gctaatcaca ataaagggct tgcctaagat gatatttggc gcgttgagct cgctaagacg
     9421 cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc cagacctgac cggtataaat
     9481 ccctatcttg cgttcttcca cgttctgatt gccatcaacc atcaatgact caggtaacca
     9541 aaaatggata aaacttcgtt tccgctccac ggttttatcc tcttcctgca ccgtataaag
     9601 ctcggtcaca ggaatagcga taagcggccc aacgagtttt tgcggcccgc tggtactttg
     9661 gcgaatcgca tcttccacat cgctacggta atcggcacgt tcgacaatca cttgccgaat
     9721 cagcattatc ggaatcaaca acagcaatac tgcaccaaac aaggtggtca ttttccagaa
     9781 caggggggat ttcaacatag tcttctcctt tgcaatggca gcaggatacg aaggctatgt
     9841 gggaagaatt tgaagctatg tgaagtgacg gtgaagtcga agcgaggcca gcacgccacc
     9901 ttcctgcacg ttgcgcagcg tgacttcgcc gttaaacaaa cgggcgacct cactgacgaa
     9961 cgccaacccc agaccgctgc ttttttgccc atttgcacgc ggcaaagagt aaaagcgttc
    10021 aaaaatacgt gacagcgcgt agtcaggaat accactaccg gtatccagca ccttaagggc
    10081 gacgtgttcc tgatccactt cggcgcttag cgttatgcga ccgctctcgg gggtaaaatc
    10141 gatggcgtta tccagtaaat tccccagcgc ctgctccagt aacgccggtt cagcagcaac
    10201 attaacctca gtgggcataa catgtagagt gatgtttttt tctgccaact gcacggtgcg
    10261 cgcttcgctg acgcggcgaa ataatgccgc cacatcaaca acagtcagaa cgacttcctg
    10321 acgattctcc agtcttgcct ggcgtagtaa cgtttctacc aatgcctgca tacgcgcatt
    10381 ttgcgtcaga atgttgtcgg taaaacgagc caccacttcc ggtggcggac cttcgcgtaa
    10441 aatttccgcc gcgccacgaa tcgccgccag tgggcttttt agctcatggg ttaacgcata
    10501 aacatactgc tcaatatagt ttttcccttc cagcttcacg cgcatacttt ccagcgcctg
    10561 cgcaagctta cgcaactcgc tactgccgag ttcaggaaga ggaacgggct tattgtcagt
    10621 gacggaatcg gcatagcgag tgagccgggc aatggagcgg ttgatccacc aaaccatgcc
    10681 tgcgccaatc accagtgcaa tccccagcaa aatggcgctg gcccataaaa ttcgccgctc
    10741 gctacgctta atgaccggag ccatcgccgc gttcggtttg cctacgctca aaacgccaat
    10801 aagccgcgag ccgtccataa tcggcgcggc aacatacatc accgaacttt cgggatcggc
    10861 aggattttgc aacgtgctgc gcgcaccata ctgaccacgc aacgttagcc agacgtcatt
    10921 ccagcgcgaa taatcctgcc caacggcttt atttgccgaa tcgaacaata ctttaccctg
    10981 cgaatcggtc atatagacat gatattcatt gcgcactttg ttaatgccac cgatattggc
    11041 gcgaaacgga cgatgttgta gctgattaaa cgcctgcgcc agttgcccat gcgttgggtc
    11101 cccagagagc aaatcgggac gcgccagctc cgccagcaac gttgcggtgt cgatcaacgt
    11161 cccctccgtt gctcttcgca cgcccggttt aacttctttg acaaaaatag ccagtacgaa
    11221 ccaggccgcc accgccacca gtaaaaaata gcccagcagc aaccgcatgc cgatacgcat
    11281 tacaggcccc tcaggctata tcccatgccg cgatgagtat taatcggtga aagatcgggg
    11341 ttgatggcac gcagcttggc atgcaacgtt ttaatgtggg tatcaacggt gcgatcgtag
    11401 gtgtcctgcg catcttccca cacgctatcc atcagttgct ggcgggacca gacgcggcct
    11461 ggcgacttga gtaacgtctt cagcaataaa aactcatacc gcgtcagcgt taatggcgtg
    11521 tcaaaccagc tgatctgcgc cgcgggttca ttcaattcaa aatgaccaat acggatgacg
    11581 ggagacggcg acgagaactt cttcacccga cgcagtaagg tgcgcaccct ggcgcacact
    11641 tcgcggggtg aaaacggttt agccacatag tcgtcagcgc caatttccag cccaagcagg
    11701 cgatcgacct cttcacttcg ggccgtcagg aacagtacag gtaacgccgg atggagcgcc
    11761 agtaactggc tgcacaattc aaagccgctg atatcaggca aaccaacgtc gagaatcatg
    11821 acgtcgggaa ccagctggcg agctttatcc agcaccggca agcctcgctc aaagacctcg
    11881 acggcaaaac cttcctgctg caacatgtag accagcgtgt cggctatccc ttgctcatct
    11941 tccactaacc agaccgtttc ccgttgtata aatcgcctct gttattgccg ccacggcatg
    12001 acaggaaccg cgctaatcgc gtttttcggc gaaccttcta caactttgtc ggagtaagcc
    12061 agataagcga gcgcgttgcg tttggcgtca taaaagcgca cgacctgtaa cgacttaaag
    12121 accagggacg tgcgtttttt gaatactacc tcgccctgag ctttgccgtt tttaatacga
    12181 tccgacagtt caatcggccc gacttgctga caagaaatag ccgcatcgga ggtatcttcc
    12241 gccagaccca atcccccttt aataccacct gttttcgccc ggctcacata acaggtgaca
    12301 tttttcacat cgggatcgtc aaaggcttcc acaacaattt tgtgatccgg gccgatcatt
    12361 ttaaatacgg tgtcgaccga accaatctct tctgcatgag ccaatggccc cagcattatc
    12421 aggcttaaag acaggatcaa atgcttgtat ttcatattgt taccattttt atctattaca
    12481 cgagcaatta gttcgtcacg gtaaataaca tattgattaa gaacaaaatc tcaattcttt
    12541 tatttccgtc aagccctaca acatactcta ctaagaaaaa aacactgaat gctaaaacag
    12601 caaaaaatgc tattatccaa ttacctgatg tcaggtgctc gttgttgaaa ggatgaggat
    12661 attttatgga tcaggccggc attattcgcg accttttaat ctggctggaa ggtcatctgg
    12721 atcagcccct gtcgctcgac aatgtagcgg cgaaagcagg ttattccaag tggcacttac
    12781 agagaatgtt taaagatgtc actggccatg ctattggcgc gtatattcgt gctcgtcgtt
    12841 tgtcgaaatc ggcggtcgca ctacgcctga ctgcgcgtcc gattctggac atcgcgctgc
    12901 aataccgctt cgactctcaa cagacattta cccgcgcatt caagaagcag tttgcccaga
    12961 ctcctgcact ttaccgccgt tctcctgaat ggagcgcctt tggtattcgc ccgccgctac
    13021 gtctgggtga attcacaatg ccagagcaca aatttgtcac cctggaagat acgccgctga
    13081 ttggtgttac ccagagctac tcctgttcgc tggagcaaat ctctgatttc cgccatgaaa
    13141 tgcgttatca gttctggcac gattttctcg gcaacgcgcc gaccattccg ccagtgctct
    13201 acggcctgaa tgaaacgcgt ccgagtcagg ataaagacga cgaacaagag gtattctata
    13261 ccaccgcgtt agcccaggat caggcagatg gctatgtact gacggggcat ccggtgatgc
    13321 tgcagggcgg cgaatatgtg atgtttacct atgaaggtct gggaaccggc gtgcaggagt
    13381 ttatcctgac ggtatacgga acgtgcatgc caatgctcaa cctgacgcgc cgtaaaggtc
    13441 aggatattga gcgatactac ccggcagaag acgccaaagc gggagatcgc ccaattaatc
    13501 tgcgctgtga actgctgatt cctatccgcc gttaacgctg cagctcatct aatgcagggg
    13561 cgtccagatg cgagatgtcc cctgcggttt caacgaccca gccagacgcc agccacaggc
    13621 tttcctgata atccacgcgc gaaatagaac agttacgcag acgtaagcgg cgcgctgccc
    13681 atgctggtaa tccgagaatc gtactcacca gacatcccag tgcaatacca tggctcacca
    13741 gcagcggtcg gcttccctgc ggtaagtccc ggcaagattc cagtgccgca ttgacgcgat
    13801 cgctgagctc ctgcattgac tcgccttcag gaatacgccc gtcaacggtg ccattgacca
    13861 gctgccgacg ccagttctct tcttcttcgg tcagagaatc gatatgtctt ttttccagca
    13921 cacccatgtt taattcacgc aggcgagaat caaagatgat gtcacagccg caggcctggg
    13981 cgatgatttc cgccgtacgc cgggtgcgtc ctaaatcgct actgataata tgcgtaatgc
    14041 caagctcttt ggcacgggtt gccacctgca tcgcctgttg ctcacctttg gcggtcagcg
    14101 ggctgtcaga ctggccctga atacgtcgct cggcgttcca ctgcgtttca ccgtggcgga
    14161 ctaggtatac ctgtaacatg ctttttttcc gtatactgct gtaacgttaa ttttctgagt
    14221 aatgctgatt atgcaccaag ttgtctgtgc gaccaccaat cccgctaaaa ttcaggccat
    14281 tctgcaggca tttcacgaga tcttcggcga aggatcctgc catattgcat ccgttaccgt
    14341 cgagagcggt gtaccggaac agccctttgg cagtgaggaa acgcgcgctg gcgcacgaaa
    14401 tcgggtagca aatgcccgcc gtttacttcc agaggctgat ttttgggtgg cgattgaagc
    14461 tggcatcgat ggtgacagca ctttcagttg ggtggtgatt gaaaacacca gccagcgcgg
    14521 cgaggcgcgt tcggctactt tgccgttacc tgcggtgatt ctgcagaaag tgcgtgaagg
    14581 tgaggcgctt ggtcccgtga tgtcgcgtta taccggtatt gatgagattg gtcgtaagga
    14641 aggggcgatc ggcgtgttta ccgccgggaa actcactcgc gccagcgtct atcaccaggc
    14701 ggtgatcctg gcgctgagtc cgtttcacaa tgccgtgtat caggcattgt aggacggata
    14761 aggcgttcac gccgcatccg gcattcggtg cacgatgcct gatgcgacgc tgccgcgtct
    14821 tatcaggcct acaaaatcaa tcgcttttca gcaacacctc ttccagccac tggcgcagct
    14881 ccacgggcgc ggctttcagg ctgttagatc cacgcgtaat cgtcgcgatg cccgcgccga
    14941 gttcattttt taactcacgc tgactcattt cgccacgcaa cagctcttcg acaatacgca
    15001 cgcgagtccc caacgcttcg cgctcatctg gcgtcagcat caggtttaac aacggtaaat
    15061 ggagatcgtt ttggtaggca ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac
    15121 gctgttctgc catcgctgct gaatagggtg attgttgggc cataatatgt cgccattgtt
    15181 agcgggggaa gcaaaatgcc tcccccggtt gtactcgcta aagagtacga tagcatatca
    15241 taaacgtgcg gatcagtaac gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc
    15301 ccccatgaaa taacggtagt aagcgtcata agccagcacg ttcttcacat aaccgcgcgt
    15361 ctcggagaac ggaatactct cgacaaatgc cacagcgtcg atacgcccgg cgctgttgcc
    15421 aagccaggtt cgcacccgtc caggtccggc gttataagct gctgaggaga aaatacggtt
    15481 attgccaaac tgctgataaa catattgcag gtaactggtg ccaatgttga tattcgtttc
    15541 cggatccagc aattgcccag ggctgctata accaggaata gagaacatct tcaccgtatg
    15601 ggtcgctgta ccaggcataa tctgcatcag gccgctggcc cctaccggtg atttcacttt
    15661 cggattccag gcgctctcct gacgggcaat cgccatcgca tagctttgcg ggatctcttt
    15721 cccactggtg taacgtttga aaagatcgtt gtaagccagc gggaatcgct cttccagatg
    15781 atcccaaagc ttcccggcga tcgttgcctg aacgctaaga tcccaccatt ggttattgaa
    15841 agcataccga gccagttgag cctgctctgt ttttgacttg ctcttcacca gattggccca
    15901 ctcgctacgc gcggtgttat cgagattcca gtacatcaac tcgcgcacgc gcgccatctc
    15961 cggcccctga gtcagggcgc tgtcaacatt ctgcggcgct ttatcaatct tcagctcata
    16021 ctcttctccg atgcgttgtg ctgcaaccat cgggtagaaa ccacgctgtt gcatgagttg
    16081 atgcaaaatc tctttcgctt cagcttcacg tccgcgttcc agcaataaat ccgcctgcca
    16141 gtaacgccat tcatctttct cttttgcttc catcggcaga cgcgccagcc aggtattcag
    16201 gccgcgacga tcgccggtgc caagtgccat tcgcacacgg cgttcaatca acgacgtgga
    16261 ttgcgagcgc ataatggcgt catcgcgcca tttcgcctgc tcgtcggtga catcgttgcc
    16321 catcaaacgc caggcgacga tatcgcgcag ctcctgaatc tgatcttcat taagctgctg
    16381 cgcctgggca agcgacggga tcatcagccg tgcattttca gcatcctgcc gcgccacact
    16441 ggcaaacgcc accgctgcca tttgacgggt aaaatcggtc gctccggttg tacgcgcgaa
    16501 ggtcagtacc gtattagggt tgttcgccag tgaaatgatt gccgaggcga tagtctggta
    16561 atcggcaggc atctgccctg ccagcactgt taccaggcca gtgttacccg ctttcatcgc
    16621 cagacggata cgctctaaat acgccagcgg atcttgttta cctgacgcac gccagacgct
    16681 aaataacttg tcacaggcgt taggctggct cttgccggtt agccacagct ctttcgcccc
    16741 ttgccaggct tcttcactct gcccggtgtt ccatttcgca tagtagtaat tacattgcgc
    16801 ttcggtagtt ccgggctttt ccgggctaaa ggctaacaag ccacgccagt cttcacgccg
    16861 cgccagttca ttgacgaaac gagattgcag cgtgcgagcg ggaggaagcg tggggttagc
    16921 gcgaacaaag ttagtgaccg tcaccgccgg ttgattcatc agatcatcgg tgatctggcg
    16981 gtattccagg tagggataaa gcggataatc cttcagtcca ggcatcattt gttcgaccac
    17041 atccatttgt cgattatccc aggcctgctt gatttgcgcg taacggctac gctgctcatc
    17101 cagtgagtcg gctcgcgcca cgctgctgac cgtcagcaga cagacaccgg cagccaacag
    17161 ccgccaggta acttgtttgg ctttttccac aagcgcatcc tctaagtgta aatacatcaa
    17221 tgcagcgtca tgccgcgtaa tagatttatg ctaaccagtc attgccgttt acgccacgtt
    17281 acggacactt ttttactttt actgcgaagg tgatcgacca actgggatta ggcggggaaa
    17341 aaggctacac ttcgcctttg aaaactcatc atcacgataa acatagaggc gaagtccaac
    17401 gtggctcaat tcgtttatac catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat
    17461 attttgaaaa acatctctct gagtttcttc cctggggcaa aaattggtgt cctgggtctg
    17521 aacggcgcgg gtaagtctac cctgctgcgc attatggcag gcattgataa agacatcgaa
    17581 ggtgaagcgc gtccgcagcc agacatcaag attggttacc tgccgcagga accgcagctg
    17641 aacccggaac acaccgtgcg tgagtccatt gaagaagcgg tttctgaagt ggttaacgcc
    17701 ctgaaacgcc tggatgaagt gtatgcgctg tacgccgatc cggatgccga ttttgacaag
    17761 ctggccgctg aacaaggccg tctggaagag atcattcagg ctcacgacgg tcataacctg
    17821 aacgtacagc tggagcgtgc ggcggatgcg ctacgtctgc cggactggga cgcgaaaatc
    17881 gctaacctct ccggtggtga gcgtcgtcgc gtagcgttgt gccgcctgct gctggaaaaa
    17941 ccagacatgc tgctgctcga cgaaccgacc aaccacctgg atgccgaatc cgtggcctgg
    18001 ctggaacgct tcctgcacga cttcgagggc accgtggtgg cgattaccca cgaccgttac
    18061 ttcctcgata acgttgcagg ctggatcctc gaacttgacc gcggtgaagg tattccgtgg
    18121 gaaggcaact actcctcctg gctggagcag aaagatcagc gcctggcgca ggaagcttca
    18181 caagaagcgg cgcgtcgtaa gtcgatcgag aaagagctgg agtgggtacg tcagggaact
    18241 aaaggccgcc agtcgaaagg taaagcacgt ctggcacgct ttgaagagct gaacagcacc
    18301 gaatatcaga aacgtaacga aaccaacgaa ctgtttattc cacctggacc gcgtctgggc
    18361 gataaagtgc tggaagtcag caacctgcgt aaatcctacg gtgatcgcct gctgattgat
    18421 gacctgagct tctcgatccc gaaaggggca atcgtcggga tcatcggtcc gaacggcgcg
    18481 ggtaaatcga ccctgttccg tatgatctct ggtcaggaac agccggacag cggcaccatc
    18541 actttaggtg aaacggtgaa actggcatcg gttgatcagt tccgtgactc aatggataac
    18601 agcaaaaccg tttgggaaga agtttccggc gggctggata ttatgaagat cggcaacacc
    18661 gagatgccaa gccgcgccta cgttggccgc tttaacttta aaggggttga tcagggtaaa
    18721 cgcgttggtg aactttccgg tggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag
    18781 gttggcggca acatgctgct gctcgacgaa ccaaccaacg acctggatat cgaaaccctg
    18841 cgcgcgctgg aaaacgccct gctggagttc ccgggctgtg cgatggttat ctcgcacgac
    18901 cgttggttcc tcgaccgtat cgccacgcac atcctggact accaggatga aggtaaagtt
    18961 gagttcttcg aaggtaactt tactgagtac gaagagtaca agaaacgcac gctgggcgca
    19021 gacgcactgg agccgaagcg tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc
    19081 cggatgcggc gtgaacgcct tatccggcct acaaaccaag aaatttcaat acgttgtgaa
    19141 tctccaggcc tgataagacg cggtaagcgt cgcatcaggc attgagcacc gaatgccgga
    19201 tacggcgtga acgccttttc cggcctacaa tagccttagt atccgcaatt tcgtagcggg
    19261 tcacccgtgc tgattctttt aaaccttgtt taatctcgac agcctcttcc agagaggttt
    19321 tcaactcatc aaaaaactta ttttatacct cgcctttgag ccgtttcatc gcggttatct
    19381 ctgctccccc atcatctccc gcaccagctc cacgcagcgc aggaaacggc tgtcataatc
    19441 gtcctcttca acccgcacga attcgatatt gttctcttcc agcatctcca ccagcaagtt
    19501 ctggaactct ttgcgatcca ccgaactgcc gaggctgcgt aaaccatccg ccacccacgg
    19561 cgtgttgttc tccagcagga tcaccagatc gaaacggtat tcatcaatca acgcctgtac
    19621 gaacggatgc tcacgccctt cgtacttttt gcagaacgcc tgggtagtga caaaatcagt
    19681 gtcaataaac gccactttat tggcatattt cactgcaaaa tcaatgtatt gtgcgtggcc
    19741 cagcgcgatt ttatcgtaat cggaatactg caatgcgatc tcatcaccgc cgaggtgtga
    19801 aaagacataa tcgcgaccat attcccacgc actggtggta ttgaagatat tggcaagttt
    19861 gtttaccagg gtggatttac cgctcgactc gccaccaagg atcgccacgg tacgtacaaa
    19921 gaacggcttc acttccgtag gaatatattc ccagtagcgg aacgggtttt cgcggatctg
    19981 cgcaccgctg atactcataa aggtacgttt cggatcgacc agcaccgtat cgatccccag
    20041 atgttccata tactgtggcg catcggcttc ttccgaggtg tagatcaggt ccggctgaat
    20101 ccccttttca gccataaact ttttgatgcc gttgctccac acatcccagc cgtgcggata
    20161 cggctccatg ccctcctcgt tgaaagcatg aatgcgaata tttttctgat atttaaaagt
    20221 ttgtaataac cagcgcagac gatccggcac cgtgggctgc tgcgacatgg cgctgtcttc
    20281 aaacaacgcg cggtcgcggg tatcgtcaaa acccataatg atatgcagct cgtcaacctg
    20341 gctacaggcg cgctggataa ggtagatatg tccggtatgc agcgggtaga acttaccgaa
    20401 tacgacacca atggttttct tctgtcgggg aaattcaagc cccaaaaaac ggtgcaacgc
    20461 ctccagcttt tgcgcgctgg ggcttttaat tttggcattc agtaactggc ttaaataccc
    20521 tttggtcata ccgctggcat cagctacctg ctgtagcgtg cagccctgtt gcttgatggc
    20581 agttttcagg taatcaaatg acgacatatc tccctccgta tctctcatta taagtcgtcg
    20641 aacacgctaa gcgcgtcgga gagtttttta acgccaaaaa tctgcatccc ttccggcgct
    20701 tttttcggta cgttagccgc cggaacaatc gcccggcgaa aaccgtgttt cgccgcttct
    20761 gaaattcgtt cctgaccgct gggcaccggg cggatctccc ctgccagccc gacttcacca
    20821 aacaccacca gatcctgtgg cagcggtctg tcacgcaggc tggaaaccat cgccagcagt
    20881 aacgctaaat cggcactggt ttcggttacc ttcacgccgc cgaccacgtt cacaaataca
    20941 tcctgatcgg ccatttgcag accaccgtga cggtgcaaga cagccagcag gattgccaga
    21001 cggttttgtt ccagccccac tgccacgcgg cgcggattcg ccatcatcga gtgatcgacc
    21061 agcgcctgaa tctccaccag caacggacgc gttccttccc acaccaccat cacggagctg
    21121 ccggaggtca cttcatctcc gcgacttaag aaaattgccg aagggttgct gacttcacgc
    21181 agcccctgtt cggtcatcgc gaagacgccc agctcattca ccgcgccgaa gcggttttta
    21241 tggctgcgca aggtgcgaaa acgggagtcg gcatcaccat ccagaagcac cgaacagtcg
    21301 atacagtgtt ccagcacttt agggccagcc agcgagccat ctttggttac gtgcccaacc
    21361 atgacaatcg ccacaccgcg cgttttggcg aagcgcgtca gataagccgc cgtttcacgc
    21421 acctgcgcca cgctaccagg cgatgattgt acatccgcca tatgcatcac ctggatcgag
    21481 tcaattacca tcagtttcgg ttgctcttct tcggcaatca ggcagatctg ttcgatgctg
    21541 gtttccgaca acatattgag attgtcagtc ggcaggccaa ggcgatgagc gcgcattgcc
    21601 acctgttgca gcgactcttc gccagtgaca tacagcgttt tcatctgctg ggccagtttg
    21661 cacagcgttt gcagtagcag cgtggatttc cccgcacctg ggttaccgcc aatcagaatg
    21721 gcgcttcccg gcaccacgcc gccgcctagt acgcggtcga actctttaaa tccggtggaa
    21781 aaacgcggca gctcttcaag gctgatatcg gagagtttct ggacttttgc caccccggcg
    21841 ctaccggcat agccgctgag acgctcgtta cgcgccaccg ttggcgacgc agcaagacgc
    21901 acctcggtga tggtgttcca ggcatgacag gcactgcact gcccctgcca gcgcggataa
    21961 tcggccccgc attcattaca aacaaaggcg cgttttggag cttttgccac ggtgtacctc
    22021 gttaatgctg tgccgcccgc aggatggcgg gcgagcaatt acttctgatt caggctgcca
    22081 gagaggatgc agaatacccc catcaggtca gcgtgacgga tggtgacttc cgccttttca
    22141 ttcacttttg gcttggcatg gtaggcaatc cccagccctg ctgctttgat catcggcagg
    22201 tcattagctc catcgccaat cgccacggtc tgcgccagcg ggatttcata ctcctgcgcg
    22261 aggcgagtca aagttttcgc tttgtactgc gcgtctacga tgtcgccgat cacattgccg
    22321 gtaaatttac cgtccatgat ctccagttca ttggctacca cggcggtcag gcgcagcttg
    22381 tcgcgcagat attcagcaaa gaaagtaaag ccgccggagg caatcgccac tttccagccc
    22441 agcgtttcca gcttgagcac cagttgcgtt aagcctggca tcagcggcag attttcacgc
    22501 acctgttgca gaatattggc gtcagcgcct ttcagcgtcg ccacacggct gcgcaggctg
    22561 gcggtaaaat cgagttcgcc gcgcatcgcc cgttcggtta cttccgccac catctcgccc
    22621 gttccggcca gtttggcaat ttcatcaata cattcaatct ggatggcggt ggaatccatg
    22681 tccatcacca gcaaacccgg cgtgcgcagg tgcgggattt tccccagcgg ggcgacatcc
    22741 agttgcgctt catgggccag gcgcgtagcc cgtgcggtga gtgaacctgc cagacgaatc
    22801 acctgataat cttccacgca ccaggcggca acaatcacca tcgccgcacc cagtttgctc
    22861 tggtattggg tcagacgctg tttatccagc ccacgaccat acagcagcca gccgctacga
    22921 cctgcgtggt aatccagtgg catcacttca tcaccactta atgaaagagg cagaccaggc
    22981 cataaagaga catcttcagg caggtcgcac caggtaatgt taggcattaa ggatcctgta
    23041 aaatcgttcg aagcagggaa aataacgcat gaggctacct tgtatccatt gcttctggca
    23101 acattaagtc tcaaattttc aaagggtgga agatggctcg cacaaaactg aaattccggc
    23161 tgcatcgggc agtgattgtc ctgttctgtc ttgccttgtt agtggcgctg atgcagggag
    23221 cgtcatggtt tagtcaaaac caccagcgac aacgtaatcc acagctggaa gaactggccc
    23281 gcaccctggc gcgtcaggta acgctgaacg ttgcaccgct gatgcgtacc gactcaccgg
    23341 atgaaaaacg cattcaggcg atcctcgatc agttaacgga tgaaagccgt atcctcgacg
    23401 cgggtgtgta tgacgaacaa ggcgatctta tcgcacgttc tggcgaaagc gtcgaagtgc
    23461 gcgaccggct ggcgctcgac ggtaaaaaag cgggcggcta ttttaaccag cagattgtcg
    23521 aaccaattgc cgggaaaaac ggaccgctcg gctatctgcg cctgacgctc gacacccata
    23581 cgctcgccac cgaagcccaa caggtggata acaccactaa cattttacgc ctgatgttgc
    23641 tgctttcact ggcaatcggc gtagtgctga cccgcacgct gctacagggt aaacgtaccc
    23701 gctggcagca atcgcccttc ctgttaaccg ccagcaaacc ggtgccggaa gaggaagaaa
    23761 gcgagaaaaa agagtgaccc attactacaa gaaaggaaat cgttatgtcc acattacgcc
    23821 tgctcatctc tgactcttac gacccgtggt ttaacctggc ggtggaggag tgtatttttc
    23881 gccagatgcc cgccacgcag cgcgttctgt ttctctggcg caatgccgac acggtagtga
    23941 ttggtcgcgc gcagaacccg tggaaagagt gtaatacccg gcggatggaa gaagataacg
    24001 tccgcctggc acggcgcagt agcggtggcg gcgcggtgtt ccacgatctc ggcaatacct
    24061 gctttacctt tatggctggc aagccggagt acgataaaac catctccacg tcgattgtgc
    24121 tcaatgcgct gaacgcgctt ggcgtcagcg ccgaagcgtc cgggcgtaac gatctggtgg
    24181 tgaaaaccgc cgaaggcgac cgcaaagtct caggatcggc ctatcgcgaa accaaagatc
    24241 gtggcttcca ccacggcacc ttgctgctca atgccgacct tagccgcctg gcaaactatc
    24301 tcaatccgga taaaaagaaa ctggcggcga aaggcattac ctcagtgcgt tcccgcgtga
    24361 ccaacctcac cgagctgctg ccggggatca cccatgagca ggtttgcgag gccataacca
    24421 aggccttttt cgcccattat ggcgagcgtg tagaagcgga aatcatctcc ccggacaaaa
    24481 cgccagactt gccaaacttc gccgaaatct ttgcccgtca gagtagctgg gaatggaact
    24541 tcggtcaggc tccggcattc tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg
    24601 aactgcattt cgacgttgaa aaaggccata tcacccgcgc ccaggtgttt accgacagcc
    24661 tcaaccccgc gccgctggaa gccctcgccg ggcgactgca aggctgcctg taccgcgcgg
    24721 atatgctgca acaagagtgc gaagcgctgt tggttgactt cccggaccag gaaaaagagc
    24781 tacgggagtt gt
//
LOCUS       DAJTNN010000046        24416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTNN010000046 DAJTNN010000000
VERSION     DAJTNN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.046"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..336)
                     /locus_tag="OO150_001653"
     CDS             complement(<1..336)
                     /locus_tag="OO150_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515839.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAA"
     gene            complement(452..1003)
                     /gene="maa"
                     /locus_tag="OO150_001654"
     CDS             complement(452..1003)
                     /gene="maa"
                     /locus_tag="OO150_001654"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR5515840.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1175..1393)
                     /gene="hha"
                     /locus_tag="OO150_001655"
     CDS             complement(1175..1393)
                     /gene="hha"
                     /locus_tag="OO150_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5515841.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1419..1793)
                     /gene="tomB"
                     /locus_tag="OO150_001656"
     CDS             complement(1419..1793)
                     /gene="tomB"
                     /locus_tag="OO150_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5515842.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2339..5488)
                     /locus_tag="OO150_001657"
     CDS             complement(2339..5488)
                     /locus_tag="OO150_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5515843.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5511..6704)
                     /gene="acrA"
                     /locus_tag="OO150_001658"
     CDS             complement(5511..6704)
                     /gene="acrA"
                     /locus_tag="OO150_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5515844.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDNKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6846..7493
                     /gene="acrR"
                     /locus_tag="OO150_001659"
     CDS             6846..7493
                     /gene="acrR"
                     /locus_tag="OO150_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5515845.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7621..10983
                     /gene="mscK"
                     /locus_tag="OO150_001660"
     CDS             7621..10983
                     /gene="mscK"
                     /locus_tag="OO150_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5515846.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLELQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIVGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYIDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11195..11356)
                     /gene="rsmS"
                     /locus_tag="OO150_001661"
     CDS             complement(11195..11356)
                     /gene="rsmS"
                     /locus_tag="OO150_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5515847.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11370..11897)
                     /gene="priC"
                     /locus_tag="OO150_001662"
     CDS             complement(11370..11897)
                     /gene="priC"
                     /locus_tag="OO150_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5515848.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            11967..12344
                     /locus_tag="OO150_001663"
     CDS             11967..12344
                     /locus_tag="OO150_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5515849.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12497..13048
                     /gene="apt"
                     /locus_tag="OO150_001664"
     CDS             12497..13048
                     /gene="apt"
                     /locus_tag="OO150_001664"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5515850.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13177..15048
                     /gene="dnaX"
                     /locus_tag="OO150_001665"
     CDS             13177..15048
                     /gene="dnaX"
                     /locus_tag="OO150_001665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5515851.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVPSALEKAP
                     AKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAARVS
                     QLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSTLKGSTVEL
                     TIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI"
     misc_feature    14441..14505
                     /gene="dnaX"
                     /locus_tag="OO150_001665"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15101..15430
                     /locus_tag="OO150_001666"
     CDS             15101..15430
                     /locus_tag="OO150_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5515852.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15430..16035
                     /gene="recR"
                     /locus_tag="OO150_001667"
     CDS             15430..16035
                     /gene="recR"
                     /locus_tag="OO150_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5515853.1"
                     /translation="MQTSPLLTQLMEALRCLSGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16145..18019
                     /gene="htpG"
                     /locus_tag="OO150_001668"
     CDS             16145..18019
                     /gene="htpG"
                     /locus_tag="OO150_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5515854.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18200..18844
                     /gene="adk"
                     /locus_tag="OO150_001669"
     CDS             18200..18844
                     /gene="adk"
                     /locus_tag="OO150_001669"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5515855.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            18976..19938
                     /gene="hemH"
                     /locus_tag="OO150_001670"
     CDS             18976..19938
                     /gene="hemH"
                     /locus_tag="OO150_001670"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5515856.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEYIEMMANLVAAYR"
     gene            complement(19935..20894)
                     /gene="aes"
                     /locus_tag="OO150_001671"
     CDS             complement(19935..20894)
                     /gene="aes"
                     /locus_tag="OO150_001671"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR5515857.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            21046..22350
                     /gene="gsk"
                     /locus_tag="OO150_001672"
     CDS             21046..22350
                     /gene="gsk"
                     /locus_tag="OO150_001672"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5515858.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22483..24159)
                     /locus_tag="OO150_001673"
     CDS             complement(22483..24159)
                     /locus_tag="OO150_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5515859.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVVSARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         6067 a         6362 c         6280 g         5707 t
ORIGIN      
        1 cgcggcaggt acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag
       61 cacgtcgata tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag
      121 caggataaat aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat
      181 caccgccagg gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg
      241 gataaaactg atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc
      301 aatcgtttcg gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa
      361 ctttccaggg aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatctacc
      421 acaattttgc ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat
      481 taccgcccac gacaacgttg tccgggacac cttttgtgac aactgcacct gaagctacca
      541 cgacgttatc accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt
      601 tattaccgat ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga
      661 tgggatgtgt tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc
      721 gaatagggca gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttgccgagaa
      781 aaatgttata gccataatca cagcgaaacg ttggctcaat ataagcctct gtcacctgac
      841 cgaataaatc agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt
      901 atcggtgaat aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc
      961 gatacaactc accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc
     1021 tgaaaatgcg gcaacacagt aagacgaaaa gtcagtgaac agaacgttcc cattacatct
     1081 gtgtgataaa gatcacatag ggtatgtctt catggtgaaa aagtataaaa ttcttaataa
     1141 acagccggtt atagctccga aagcgaatta tttattagcg aataaatttc catactgagg
     1201 aagggatctt gtcgtatagt ttattcatgg tcaattcggc gaggcggtga tctgcggctg
     1261 agtaaaatac cgccagttca ttatctgata attcgtattt atttttctcg ataacacgct
     1321 ccagcgtgtc aattgtctgg caacgacgta aacgcattaa ataatcggtt ttcgttaaag
     1381 gtttttcgga catacttcta cccatggttg taataattct aacaagataa actcgcagga
     1441 ttctctttcg tcgcattgac aaaacaacgg aatagtcgat tacctgactt ccgccatttc
     1501 tgaagatcct gcatattaat accataacta ctgaacaaca taaaggtgtc atccagatat
     1561 tcgtcgatct gctcaatgag cttattgtct tcattatact taattttgta attaagtgcg
     1621 aaggtcgcaa tatgctcaat cagttcattc aactggaggt tgatcgccga ggttgggtcg
     1681 ttaacccagc cataattgct ttcttcaagg tttgcaaggc agtcatgata cagggtttca
     1741 cagagaaact taagctgtgc gatatcatgt cttttgggtg agtattcatc cataacgcgt
     1801 ccccttctta gcggttgaac taacggacac ctttcgggat ggaaaaaact tactgacctg
     1861 gacttgccct ctcgtttgtt agcttaacta taagccactc tttgcaggta ttcatcgcat
     1921 tattacgaaa aattacaatt aggaaggaat cgggacagga ctcacttttt catactagca
     1981 ctaacccgca gcaggtaaaa gcagtttgct cgaactgttc ataacatttc aggattatcg
     2041 ccagatgaaa ttggtgacac taataccagg attgctctga atatgacgta ataaccgagg
     2101 aatgaataaa gaattaccgc aaataattaa gaatagcctc taaatgatta tggattatta
     2161 aatctattag cgctacttaa tataaattaa ctaacaatca gtatttttta tgaattttat
     2221 ccgtggttaa tactggtttt cgtatgagat cctgagttgg tggttcaatt actccttaat
     2281 gttcgtaggt tatgcataaa aaaggccgct tgcgcggcct tagtgattac acgttgtatc
     2341 aatgatgatc gacagtatgg ctgtgctcga tatcttcatt cttgcggcta aagcggcggc
     2401 gaaccaccac aaagaatacc ggaacgaaga agattgccag taccgttgcg gtcaccatcc
     2461 cgcccattac accggtacct actgcgttct gcgcgccgga accagcacca gtactgataa
     2521 ccagcggcat aacgccgagg ataaacgcca gcgaggtcat caggatcgga cgtaaacgca
     2581 tccgcactgc atcaagcgtc gcttcaatca gacctttacc ttctttatcc atcaagtctt
     2641 tggcgaattc gacgataagt atcgcgttct tcgccgacaa cccaatggtt gtgagcaggc
     2701 ctacttggaa gtaaacgtca ttggtcaggc cacggaaggt ggcagccagc aacgcaccga
     2761 taacccccag cggaacgacc agcataacgg agaacggaat cgaccagctc tcgtacagcg
     2821 ccgctagaca caggaatacg acaatcaacg aaatcgcgta cagtgaaggt gcctggttgc
     2881 cggagaggcg ttcctgatag gacatccccg tccagtcata gccaacaccg gtaggcagtt
     2941 tgctcgccag ttgttccatt agctccattg cttcaccggt acttttaccc ggtgccgcct
     3001 ggcctaagat ttccatggat ggcaggccat tgtaacgttc cagacgcggc gaaccgtact
     3061 cccaacgaga agaggagaat gccgagaacg gcaccatctg accatcagca gcacgaacat
     3121 accagtcgcc gatatcatcc ggcagcatac ggtatttcgc ttctgacatg acgtaaactt
     3181 tcttcacacg accgcggtcg ataaagtcgt tcacatagct accgccccat gcagcgccca
     3241 gagtggtgtt aatgtcgttg atagaaacac ccagcgcctg cgctttttcc tggtcgatat
     3301 caatcttaaa ctgcggggta tcttccagac cgtttggacg tacgctggtc aacatatcag
     3361 gatgctttgc tgcttctgca agcagctggt tacgcgcctg agtcagtttt tcgtgaccaa
     3421 ggccagcctg gtcaatcagc tcaaagtcaa agccggttgc agtacccagt tccacgattg
     3481 cgggcaggtt aaaggcgaaa accatcgcat ctttgatttg cgagaaagcg cgtgttgcac
     3541 gcatggtaat cgcttcaact ttgttttctt cgcccggacg atcggcccag tccttcaagg
     3601 aaacgaacgc aataccggta ttctgaccac gtcccgcaaa gccgaagccg ttaacggcga
     3661 acaccgactc aacgttgttc ttttctttgg tcagatagta atgcgttacc tcattgagca
     3721 ctttctgcgt acgttcctgc gttgcacctg ctggcagctg aaccatggtc ataaatacgc
     3781 cctggtcctc atctggcaag aaggagcttg gcagacgcac gaacagatag gccatgccga
     3841 ccacgatgat cagatacagc accaggtaac gccccgtact gcgcagaata ccgcctacgc
     3901 tgtcggtgta gtggtgcgtg ctcttctcga acatgcggtt aaaccagccg aagaagcctt
     3961 ttttaccttc cccgtgatcg cctttggcaa tcggtttcag catggtggca caaagagccg
     4021 gagtcaggat caacgctacc agtaccgaca gcgccattgc tgaaacaatg gtaatagaga
     4081 actgacgata gatagcacca gtagaaccgc caaagaaggc catcggtacg aataccgctg
     4141 acagtaccat cgcgataccg accagagcgc cctgaatctg ccccatcgac ttacgggtag
     4201 cttcttttgg cggcaaacct tcttccgcca taacacgctc cacgttttct accacaacga
     4261 tagcgtcatc caccaacaag ccgatggcga gcaccatccc gaacattgtt agcgtgttta
     4321 tcgagaagcc aaaggcggca aggacggcaa aggtcccgag caataccacc ggtacggcaa
     4381 tggtcggaat caacgtcgcg cggaagttct gcaggaacag atacataacc aggaacacga
     4441 ggatgatcgc ttcgaccagc gttttaacca cttcgtgaat agagattttc acgaacggcg
     4501 tggtgtcgta cgggtaaaca attttcagac ccgacgggaa gaacggttcc atcttcgcca
     4561 gttcagcacg gattgccgca gcggtatcca gcgcatttgc accggtcgcc agcttgatcc
     4621 ccagaccgga agccggttgg ccgttaaact ctgcgatgat gtcgtagttc tcaccaccca
     4681 gctcaatctt cgccacatca cgcagcagga cgcgggaacc atcctgattc actttcagca
     4741 ggattttgcc gaactcttca gtagaggtca gacgcgtctg agcaataata gaggcgttaa
     4801 gctgttggcc tttcaccggc ggcgtaccac cgagctgacc cgccgcaacc tgggcgttct
     4861 gcgctttgat ggcggtaatg acatcaaccg gcgttagctg gaatttgttc agctcattcg
     4921 ggttcatcca gatacgcatc gcgtactgtg aaccgaacaa ctgaacatca cccacgcccg
     4981 acgtacggct gatggcatct ttcatattcg ccgctacgta gtcagagata tcctcctgcg
     5041 tcatggtgcc atcggtgttg ataacgccga caaccatcag gaagctgctg gatgatttct
     5101 caacgctcac cccttgctgc tgaacttctt gcggcagcaa cggcatcgcc agctgcagtt
     5161 tgttctgcac ctgaacctgc gcgatatccg catcagtacc agactcaaag gtcagggtga
     5221 tctgcacggt ccccgtggag tcactgttag aggacatgta catcaggtta tcgataccgt
     5281 tcatattctg ttcgataacc tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccgg
     5341 ggtaggaggc ggagatcgtt actgccggcg gtgcaatcgt aggatattgc gccaccggca
     5401 gtttgaggat cgccagcccc cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa
     5461 tcgggcgatc gataaagaaa ttaggcatgt cttaacggct cctgtttaag ttaagacttg
     5521 gactgttcag gctgagcacc gcttgcggct tgctggttat tatcagcggt aacttcttgt
     5581 gcttttacct ggacaccagg acgcactttc tgcagcccac ttattactac gcgatcgcct
     5641 gctttcagac cttctgtcac cagccactta tcgccaatag cctggcttgc aacgatcgga
     5701 cgggtttcca ctttgtcatc cgcgccaact accagtacgg tggcatcacc acgcggcgta
     5761 cgggttacgc cctgttgcgg gactaaaata gcgtttggat taagcccttc ttccagacgt
     5821 gcgcgcacga acatacccgg cagcagagtg tgatccgggt tcgggaagat agcgcgtagg
     5881 gtgatagacc cagtggtctg atcaacggta acgtcagaga attccagcgt accgtcctgc
     5941 gggaacttaa tgccgtcact ggtgatcagc gacactttgg ctttgccgtt ctcttgtttc
     6001 agcgtgccat tcgccagttc ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca
     6061 tcaacgtaga tcggatcaag ttgctgcacg gttgccagcg cagtcgcctg accgttctgt
     6121 accaatgcgc cttccgtcac gttcgactta ccaatgcgac cgctaatcgg agcggtgact
     6181 ttggtgtaag ccagattgat ccgcgcagtt tcaacggcag ctttcgccgc agttaccgca
     6241 gcattcgcct gttgcgcatc agccagagcc tgatcgtact cttgcttact gatgtactga
     6301 gtaccgagca gtttctgata acgattcacc gtcaattgcg cgatattggc tgcagcctgg
     6361 gctttcgcca gatcaccttt cgcactgtcg tatgtcgcct gataggtcgc aggatcaatc
     6421 tgatagagag agacaccagc ttcgatgtcg ctaccttctt tgaaattacg cttcaggata
     6481 atcccgctaa cttgaggacg aacttctgcg atccggtagg cactggtgcg acccggaagc
     6541 tcggttgtga tctgcagagg ttcagttttg actgttacta cgccaacggc gggcatctgc
     6601 tggccacctt gttgggcctg tttgttgtca catcctgtta gggctaagct gcctgagagc
     6661 atcagaacga ccgccagagg cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg
     6721 tccgatttca aattggtcaa tggtcaaaag ttaataaacc cattgctgcg tttatattat
     6781 cgtcgtgcta tggtacatac attcacaaat gtatgtaaat ctaacgcctg taaattcacg
     6841 aacatatggc acgaaaaacc aaacaagaag cgcaagaaac gcgccaacac atcctcgatg
     6901 tggctctacg tcttttctca cagcaggggg tatcatccac ctcgctgggt gagattgcaa
     6961 aagcagctgg cgttacgcgc ggtgcaatct actggcattt taaagacaag tcggatttgt
     7021 tcagtgagat ctgggaactg tcagaatcca atattggtga actagagctt gagtatcagg
     7081 caaaattccc tggcgatcca ctctcagtat taagagagat attaattcat gttcttgaat
     7141 ccacggtgac agaagaacga cgtcgattat tgatggagat tatattccac aaatgcgaat
     7201 ttgtcggaga aatggctgtt gtgcaacagg cacaacgtaa tctctgtctg gaaagttatg
     7261 accgtataga acaaacgtta aaacattgta ttgaagcgaa aatgttgcct gcggatttaa
     7321 tgacgcgtcg ggcagcaatt attatgcgcg gctatatttc cggcctgatg gaaaactggc
     7381 tctttgcccc gcaatctttt gatcttaaaa aagaagcccg cgattacgtc gccatcttac
     7441 tggagatgta tctcctgtgc cccacgcttc gtaatcctgc cactaacgaa taaccctgaa
     7501 tctgactcca ggatttttcc tggacatttt cgtcgttgct attctggttc actgcgtcgt
     7561 gatattcttg cggtttgact ttttcaggtc gttcttcagg ttcagaaacc ttcattcatc
     7621 atgactatgt tccagtatta caaacgatca cggcattttg ttttttcagc atttattgct
     7681 tttgtttttg tcttgttatg ccagaacacg gcgtttgcgc gggcgtcatc gaatggtgat
     7741 ctgccgacaa aagcggacct gcaggcgcaa cttgactcac taaataaaca aaaagatctt
     7801 tctgctcagg acaaactgga gctgcaggat ctgacagata cattagccac cctcgataaa
     7861 atcgatcgca taaaagaaga gacagttcag ctacggcaaa aagtcgctga agcgccggaa
     7921 aaaatgcgcc aggcgaccgc ggcgttaaca gcacttagcg atgtcgataa cgacgaagaa
     7981 acgcgcaaaa ttctgagtac gctttcgttg cgccagctgg aaactcgcgt tgcccaggcg
     8041 ctggacgatt tgcaaaacgc acaaaacgat ctggcgtctt ataacagcca gctggtttcg
     8101 ttacagacgc agcccgaacg cgtgcaaaat gcgatgtata acgcttcgca gcagctgcaa
     8161 caaattcgca gtcgtctgga tgggactgat gtcggcgaga ctgccttacg tcccagccag
     8221 aaagtgttaa tgcaggctca gcaggcgttg ctgaatgcgg agattgacca gcagcgtaaa
     8281 agcctggaag ggaacaccgt cttgcaggat accttgcaaa agcaacgtga ttacgtgacg
     8341 gcgaacagcg cccgtctgga gcaccagttg cagctgttgc aagaagcggt aaacagcaag
     8401 cgcctgactt taactgaaaa aacggcgcag gaagccgtct ccccggatga agccgcgcgt
     8461 attcaggcta atccgctggt gaaacaggaa ctggaaatta accagcagtt aagtcagcgt
     8521 ctgattaccg cgactgaaaa cggtaatcag ttgatgcagc aaaacattaa agtcaaaaac
     8581 tggctggagc gggcgctgca atcggaacgc aatattaaag agcagattgc cgtcctgaag
     8641 ggcagcctgc tgttgtctcg tatcctttac cagcaacaac aaacgctgcc ctcggcggat
     8701 gaactggaaa acatgaccaa ccgcatcgcg gatttgcgtc tcgaacagtt tgaagttaac
     8761 cagcagcgtg atgcactctt ccagagcgat gcgttcgtca acaaactgga agaaggtcac
     8821 accaacgaag tcaacagcga agttcacgat gcgttattgc aagtggttga tatgcgtcgc
     8881 gaattgctgg atcaactcaa caaacagttg ggtaaccagc tgatgatggc cattaacctg
     8941 caaatcaacc agcagcagtt aatgagcgta tcgaaaaacc tgaaatccat cctgactcag
     9001 caaatctttt gggtgaacag taaccgtcca atggactggg actggatcaa agcgttcccg
     9061 caaagcctga aagatgaatt taagtcgatg aaaatcacgg tgaactggga aaaagcctgg
     9121 cctgccgttt ttatcgcttt cctcgccggt ttgccgctgc tgttgattgt cgggctgatc
     9181 cactggcgtc tgggctggct gaaagcgtat caacaaaaac tggcttccgc tgtgggttcc
     9241 ctgcgtaacg atagccagct caacacacca aaagcgatcc ttatcgacct gatccgcgcg
     9301 ctgccggtgt gcctgattat tctcgcggtt ggcttgattc tgttgaccat gcagctcaac
     9361 atcagcgaac tgctatggtc gttcagcaaa aaactggcga ttttctggct ggtgtttggc
     9421 ctgtgctgga aggtactgga gaagaacggc gttgccgtgc gtcacttcgg catgccggaa
     9481 cagcagacca gccactggcg tcggcaaatt gtccgcatca gtctcgcatt gctgcctatc
     9541 catttctggt ctgtggtggc agaactttcc ccgctgcatc tgatggatga tgtgctgggg
     9601 caagcgatga ttttcttcaa cctgctgctg attgccttcc tggtatggcc aatgtgccgc
     9661 gaaagctggc gtgataaaga gtcgcacacc atgcgactgg tcaccattac cgtgctgtcg
     9721 attatcccga ttgcattgat ggtgctgact gccacgggtt atttctacac cacgctgcgc
     9781 ctctccgggc gttggattga aaccgtttat ctggtgatca tctggaactt gctctaccag
     9841 acggtattgc gcggcttaag cgtagcggcg cggcgtatcg cctggcgtcg tgcgctggcg
     9901 cgtcggcaga atctggtgaa agagggcgca gaaggtgccg aaccgccgga agaacccacc
     9961 attgcactgg aacaggttaa ccagcagacg ctgcgtatta ccatgttgct gatgtttgcg
    10021 ctgttcggtg tcatgttctg ggcaatttgg tccgatttga tcaccgtgtt cagctatatc
    10081 gacagcatta cgctctggca ttacaacggt actgaagctg gcgctgcggt ggtgaaaaac
    10141 gtcaccatgg gcagtctgtt gtttgcgatt atcgcctcaa tggtggcctg ggcgttgatt
    10201 cgcaacctgc ctggtttgct ggaagtgctg gtgctctcgc gactgaatat gcgccagggc
    10261 gcgtcgtatg ccattactac catccttaac tacatcatta ttgccgttgg tgcgatgacg
    10321 gtgttcggat cgctgggcgt ctcttgggat aaactccagt ggctggcggc agcattatcc
    10381 gtaggtcttg gttttggttt acaagaaatt ttcggtaact tcgtctccgg tttgatcatt
    10441 ctgttcgaac gtccggtgcg tattggcgat acggtaacca ttggtagctt ctcggggacg
    10501 gtaagtaaga tccgtattcg tgcgacaacg attaccgatt tcgatcgcaa agaagtgatc
    10561 atcccgaaca aagcgtttgt taccgagcgt ctgatcaact ggtcgttgac tgacactact
    10621 acgcgtctgg tgatccgtct cggcgtggcc tatggctccg atctggaaaa agtgcgtaaa
    10681 gtgttactga aggcggcgac tgagcaccca agggtgatgc acgaaccaat gccggaagtc
    10741 ttctttacgg cgtttggtgc cagcacgttg gatcatgagc tgcgtctgta tgtgcgtgaa
    10801 ctgcgtgacc gtagtcgtac tgtcgatgag ctgaaccgta ctatcgatca gctgtgccgt
    10861 gaaaacgaca tcaacattgc ctttaaccag cttgaagtgc atctgcacaa cgagaagggc
    10921 gatgaggtga cggaagtaaa acgcgactac aaaggcgatg acccgacgcc agcggtaggg
    10981 taataaccaa agggggcgac atttagttgc cccgagatta ctgacaaagt gcgcgttgtt
    11041 catgccggat gcggcgtgac cgccttatcc ggcctacgaa accgcaagaa ttcaatgtat
    11101 tgcaggagcg gtgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgcccaca
    11161 atcttagggg acatttagcg gccccattta tttctcactt ttccgcctca tcatcgcgcg
    11221 ttaatttctt ttcataatcg cgttttacaa tatccagcgc gcgcagcacg gtgcgggctg
    11281 ggatctgatt ttcttccagc agcacaatca aatcgacagc cagtttgaca tcgtcagggg
    11341 cattttccag tgacatactc tctccattgc tagcgggtta agcgcgctag cctgttttcg
    11401 attttttcca gcgcatggcg gcagcgtgcc aggcgcgctt cataggcttc cacttcacga
    11461 tgcagcgttt gctgttccac gagatcggtc acccgcgcca ggcgggctct gcgttcggca
    11521 accatctcac gtagccgccg ctcaaaatcc tgatgctgaa tacgtttacg ctgccagcgg
    11581 gcaattttcg gtggcgcact gtcccactcg cgcaatgacc aggcggaggc ttcacgcgcg
    11641 atggcttcca gttgtgccgc cagatgttcc gccagccagg ccacttgcgg cagctgttgc
    11701 tgctcaactg catgacgaag cgcagccaga ttatcgccag cctcatcgag acaagcctgc
    11761 agtgttgtcg tacgggtttg aaaaaggtgt ctgtcaaaac gggcacttag cgtggcaaat
    11821 tgcgctaccg gggcacaacg ctgacgcagc gtagcgagct gtccttccag tttttccagc
    11881 agcagggcgg ttttcacgat aagacctcaa tgaaaatgat aattgttatg ctaaagtagc
    11941 cactctgtaa gctgacaaca ctcaatatgc aacgaatcat tttaatcatc attggctggc
    12001 tggcggtagt gctgggtacg ctgggcgtgg tattaccggt attaccgacg acgccgttta
    12061 tcctgctggc ggcctggtgc tttgcccgtt cttccccgcg ctttcacgcc tggttgctgt
    12121 accgctcatg gtttggcagc tatctacgtt tctggcagaa acatcatgcg atgccgcgcg
    12181 gcgtcaaacc gcgggcgatt ttgcttattt tgctcacgtt tgccatttct ctgtggttcg
    12241 tccagatgcc atgggtgcgc atcatgttgc tggtaattct cgcctgtttg cttttctata
    12301 tgtggcgaat tccggtgatt gatgaaaagc aagaaaagca ctgaagcaca acaatcgcag
    12361 ttgcaattat tgcgtacagc cagtacattc tggcgttttc gagcacaggc gcaggcggtc
    12421 aaaggttaaa caactgttac ttttgatacg tttaaaacgc gccgtgagta ccaccgtaac
    12481 aagcaggcat acacttatga ccgcgactgc acagcagctt gagtatctca aaaatagcat
    12541 caaaagcatt caggactacc caaaacccgg cattcttttc cgcgatgtca ccagcttact
    12601 ggaagacccg aaagcttacg ctctcagcat cgacttgctg gttgagcgtt acaaaaatgc
    12661 gggcattacc aaagttgtcg gcaccgaagc gcgtggcttc ttgtttggcg ctccggtagc
    12721 tctgggtctg ggcgttggct ttgtaccggt ccgtaaaccg ggcaaactgc cgcgtgaaac
    12781 catcagtgaa acttacgacc tggaatacgg caccgatcag ctggagatcc acgttgatgc
    12841 catcaaaccg ggcgacaaag ttctggtggt ggacgatctg ctggcaaccg gcggcactat
    12901 cgaagcgacc gttaaactga tccgtcgtct gggtggtgaa gtggctgacg ctgcgttcat
    12961 tatcaacctg ttcgatctcg gcggcgaaca gcgtctcgaa aaacagggca ttaccagcta
    13021 cagccttgtc ccgttcccgg gccattaatt atcgccagtc ttgtgctgcc cacgctacgg
    13081 acagcacaag atgtgcattc agcctcgccg ttctgacggg gctgtgttag cattacccct
    13141 tcgtgaatcc accttccagc gtttcagagc ctgccaatga gttatcaggt cttagcccga
    13201 aaatggcgcc cacaaacctt tgctgacgtc gtcggccagg aacatgtgct gaccgcactg
    13261 gcgaacggct tgtcgttagg gcgtattcat catgcttatc ttttttccgg tacccgtggc
    13321 gtcggaaaaa cctctatcgc ccgactgctg gcgaaggggc taaactgcga aaccggcatt
    13381 accgcgacgc cgtgcggcgt gtgcgataac tgtcgtgaaa tcgagcaggg gcgctttgtc
    13441 gatctgattg aaatcgacgc cgcctcgcgc accaaagttg aagatacccg cgacctgctg
    13501 gataacgtcc agtacgctcc ggcgcgtggt cgtttcaaag tttatctgat cgacgaagtg
    13561 catatgctgt cgcgccacag ctttaacgca ctgttaaaaa cccttgaaga gccgccggag
    13621 cacgttaagt ttctgctggc gacgaccgat ccacagaaat tgccggtgac gattttgtcg
    13681 cgctgtttgc aatttcatct caaggcgctg gatgtcgagc aaattcgcca tcaacttgag
    13741 cacatcctca acgaagaaca tatcgctcac gagccgcggg cgttgcaatt gctggcgcgc
    13801 gccgctgaag gcagcctgcg agatgcctta agtctgaccg accaggcgat tgccagcggt
    13861 gacggccagg tttcaaccca ggcagtcagt gcgatgctgg gtacgcttga tgacgatcag
    13921 gcgctgtcgc tggttgaagc gatggtcgag gccaacggcg agcgcgtaat ggcgctgatt
    13981 aatgaagccg ctgcccgtgg tatcgagtgg gaagcgttgc tggtggaaat gctcggcctg
    14041 ttgcatcgta ttgcgatggt acaactttcg cctgctgcac ttggcaacga catggccgcc
    14101 attgagctgc ggatgcgtga actggcgcgc accataccgc cgacggatat tcagctttac
    14161 tatcagacgc tgttgattgg tcgcaaagaa ttaccgtatg cgccggaccg ccgcatgggc
    14221 gttgagatga cgctgctgcg cgcgctggcg ttccatccac gtatgcctct gcctgagcca
    14281 gaagtgccgc gccagtcctt tgcacccgtc gcgccaacgg cagtaatgac gccaacccag
    14341 gtgccgccgc aaccgcaatc agcgccgcag caggcaccga ctgtaccgct cccggaaacc
    14401 accagccagg tgctggcggc gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca
    14461 aaaaagagtg aaccggcagc cgctacccgc gcgcgtccgg tgccatcggc gctggaaaaa
    14521 gcgccagcca aaaaagaagc gtatcgctgg aaggcgacca ctccggtgat gcagcaaaaa
    14581 gaagtggtcg ccacgccgaa ggcgctgaaa aaagcgctgg aacatgaaaa aacgccggaa
    14641 ctggcggcga agctagcggc agaagccatt gagcgcgacc cgtgggcggc acgggtgagt
    14701 caactttcgc taccaaaact ggtcgaacag gtggccttaa atgcctggaa agaggagagc
    14761 gacaacgcag tatgtctgca tttgcgctcc tctcagcggc atttgaacaa ccgcggtgca
    14821 cagcaaaaac tggctgaagc gttgagcacg ttaaaaggtt caacggttga actgactatc
    14881 gttgaagatg ataatcccgc ggtgcgtacg ccgctggaat ggcgtcaggc gatatacgaa
    14941 gaaaaacttg cgcaggcgcg cgagtccatt attgcggata ataatattca gaccctgcgt
    15001 cggttcttcg atgcggagct ggatgaagaa agtatccgcc ccatttgatc gtaagcacag
    15061 cttacgttcg tcatccttaa cgtgattgag agagaaacct atgtttggta aaggcggtct
    15121 gggtaacctg atgaagcaag cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga
    15181 gatcgcgcag ctggaagtca ccggcgaatc tggcgcaggt ctggtaaaag tgaccatcaa
    15241 cggtgcacac aactgccgtc gcgtagagat cgacccgagc ctgctggaag acgacaaaga
    15301 gatgctggaa gacctggtgg ctgcagcatt caacgacgca gcacgtcgta ttgaagaaac
    15361 gcagaaagaa aaaatggcct ctgtatcctc cggaatgcag ctgccgcctg gctttaagat
    15421 gccgttctga tgcaaaccag cccgctgtta acacagctta tggaagcact gcgctgtctg
    15481 tcgggcgttg gcccgaagtc ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt
    15541 agcggcggga tgcgtctggc gcaggcgctc acccgggcga tgtcggaaat cggccactgc
    15601 gccgattgcc gcactttcac cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt
    15661 caggaaaacg gtcaaatctg tgtggtggag agtccggcgg acatctacgc cattgagcag
    15721 acggggcagt tttctggtcg ttattttgtg ttgatggggc atctgtcacc gctggacggc
    15781 atcggtccgg atgatatcgg gcttgatcgt ctggaacagc gtctggcaga ggaaaaaatc
    15841 actgaagtga tcctcgccac caatcccacg gttgaaggtg aagctaccgc taactacatt
    15901 gccgagcttt gcgcgcaata tgacgtggaa gctagccgaa tcgctcatgg cgtaccggtt
    15961 ggcggcgaac tggaaatggt cgatggcacc acgctgtcac actcccttgc cgggcgtcat
    16021 aagattcgtt tttaagcaaa cgagagcagg atcacctgct ctcgcttgaa attattctcc
    16081 cttgtcccca tctctccctc atcctgtttt taaccttaaa atggcattat tgaggtagac
    16141 ctacatgaaa ggacaagaaa ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct
    16201 gatgatccat tctctctatt ccaataaaga aatcttcctg cgtgagctta tctctaacgc
    16261 ctccgatgcg gcggacaagc tgcgtttccg tgcgctatct aacccggacc tgtacgaagg
    16321 tgatggcgaa ctgcgcgttc gtgtctcttt cgataaagac aagcgcacgc tgaccatctc
    16381 cgataacggc gtggggatga cccgcgacga agtgattgac catctgggga ctatcgctaa
    16441 atccggtacc aaatcattcc tcgaatccct gggttctgac caggcgaaag acagccagct
    16501 gatcggtcag tttggtgttg gtttctactc tgcgtttatc gtggccgaca aagtgactgt
    16561 gcgtactcgc gcggcaggcg aaaaaccaga aaacggcgtc ttctgggaat cggctggcga
    16621 aggtgaatat accgttgccg acatcaccaa agaagatcgt ggtactgaaa tcaccctgca
    16681 tctgcgtgaa ggcgaagacg agttcctcga tgactggcgc gtgcgttcca tcatcagcaa
    16741 atactccgac catatcgcgc tgccggtaga gatcgaaaaa cgcgaagaga aagacggcga
    16801 aaccgttatc tcctgggaga aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc
    16861 ggaaatcacc gatgaagagt acaaagagtt ctacaaacac atcgcccacg actttaatga
    16921 tccgctgacc tggagccaca accgcgttga aggtaagcag gagtacacca gcctgctgta
    16981 catcccgtcc caggctccgt gggatatgtg gaaccgtgat cataaacacg gcctgaaact
    17041 gtacgtccag cgtgtgttca tcatggacga cgcagaacag ttcatgccga actatctgcg
    17101 cttcgtgcgt ggtctgattg actccagcga tctgccgctg aacgtttccc gtgaaatcct
    17161 ccaggacagc acggtaacgc gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat
    17221 gctggaaaaa ctggcgaaag acgacgcgga aaaataccag accttctggc aacagtttgg
    17281 cctggtactg aaagaaggtc cggcggaaga tttcgctaac caggaagcga tcgccaaact
    17341 gctgcgtttt gcttctaccc ataccgattc ttctgcgcag accgtatctc tggaagacta
    17401 cgtttcccgc atgaaagaag ggcaggagaa aatctactac atcaccgcag acagctatgc
    17461 ggcagcgaag agcagcccgc acctggaact gctgcgtaag aaaggcatcg aagttctgct
    17521 gctttccgac cgcatcgatg agtggatgat gaactatctg actgagttcg acggtaaacc
    17581 gttccagtcg gtgtctaaag ttgacgagtc gctggaaaaa ctggctgacg aagttgatga
    17641 gagcgcgaaa gaagcggaga aagcactgac tccgttcatc gaccgtgtga aagccttgct
    17701 cggcgagcgc gtgaaagatg tccgtctgac tcaccgtctg accgatacgc cagcgatcgt
    17761 ttcgaccgac gcggacgaaa tgagcactca gatggcgaaa ctgttcgctg cggcgggcca
    17821 gaaagtgcca gaagtgaaat acatcttcga actgaacccg gatcacgtac tggtgaaacg
    17881 tgcggcagat actgaagatg aagcgaagtt cagcgagtgg gtagaactgc tgctggatca
    17941 ggcgctgctg gcagaacgcg gcacgctgga agatccgaac ctgtttattc gtcgtatgaa
    18001 ccagctgctg gtttcctgat gtcatgccgg atgacctttg tgtcatccgg catttttctt
    18061 ttcatcatct gcactttccg caaattatct cgccattaac cgtttcagcc tcaggtgcct
    18121 ttcttgaggc aatcgcctgt tggtggtatc gtttatcgct ttttcaaaaa attcgacaca
    18181 ttttaagggg attttcgcaa tgcgtatcat tctgcttggc gctccgggcg cggggaaagg
    18241 gactcaggct cagttcatca tggagaaata tggtattccg caaatctcca ctggcgatat
    18301 gctgcgtgct gcggtcaaat ctggctccga gctgggtaaa caagcaaaag acattatgga
    18361 tgctggcaaa ctggtcaccg acgaactggt gatcgcgctg gttaaagagc gcattgctca
    18421 ggaagactgc cgtaatggtt tcctgttgga cggcttcccg cgtaccattc cgcaggcaga
    18481 cgcgatgaaa gaagcgggca tcaatgttga ttacgttctg gaattcgacg taccggacga
    18541 actgattgtt gatcgtatcg taggccgccg cgttcatgcg ccgtctggtc gtgtttatca
    18601 cgttaaattc aatccgccga aagtagaagg caaagacgac gttaccggtg aagaactgac
    18661 tacccgtaaa gacgatcagg aagaaaccgt acgtaaacgt ctggttgaat accatcagat
    18721 gactgcaccg ctgatcggct actactccaa agaagcggaa gcgggtaaca ccaaatacgc
    18781 gaaagttgac ggcaccaagc cggttgctga agttcgcgct gatctggaaa aaatcctcgg
    18841 ctaatcaagc cgcagcagat ctgctttcgg gcaggtctgc tcatccacct caggcaatac
    18901 ctctcacagc aattagttct tcttcctcac ttttccgcta caattatcaa caagttgaat
    18961 cgataagagg cggtaatgcg tcagactaaa accggtatcc tgctggcaaa cctgggtacg
    19021 cccgatgccc ccacacctga agcggtaaaa cgctatctga aacaattttt aagcgacaga
    19081 cgcgtggttg atacctcacg gttgttatgg tggccattgc tgcgcggcgt tattttgccg
    19141 ctgcgctcgc cgcgtgtggc gaagctgtat gcctctgtct ggatggaagg tggctcgccg
    19201 ctgatggttt acagccgcca gcaacagcag gcgctggcac aacgtttacc ggagacgccc
    19261 gtagcgctgg gaatgagcta cggctcgcca tcactggaaa gcgccgtcga tgaactcctg
    19321 gcagagcatg tagatcatat tgtggtgctg ccgctttatc cgcaattctc ctgttctacg
    19381 gtcggtgcgg tatgggatga actggcacgc attctggcgc gcaaacgtag cattccgggg
    19441 atatcgttta ttcgtgatta cgccgataac cacgattaca ttaatgcact ggcgaacagc
    19501 gtacgcgctt cttttgccaa acatggcgaa ccggatctgc tgctgctctc ttatcatggc
    19561 attccccagc gttatgcaga tgaaggcgat gattacccgc aacgttgccg cacaacgact
    19621 cgcgaactgg cttccgcact ggggatggca ccggaaaaag tgatgatgac ctttcagtcg
    19681 cgctttggcc gggaaccctg gctgatgcct tataccgacg aaacgctgaa aatgctcgga
    19741 gaaaaaggcg taggtcatat acaggtgatg tgcccgggct ttgctgcgga ttgtctggag
    19801 acgctggaag agattgccga gcaaaaccgt gaggtcttcc tcggtgccgg cgggaaaaaa
    19861 tatgaatata ttccagcgct taatgccacg ccagaatata tcgaaatgat ggctaatctt
    19921 gttgccgcgt atcgctaaag ctgagcggta aagaactgag cgccgtcgcg aagagcctcg
    19981 tcggcggttt tcatcattcg tgaataatgc aaaaaggcat gcagcgtacc tgggtagagt
    20041 ttgaactcac agggctgctg atgcgccgct aacgtctggt aaagcagacg gctgtcatcc
    20101 agcagcggat cgaactccgc cccggcaata aaacagggcg gaacttcgcg agtgagatca
    20161 ttattaaaca gacagtaata cggtgactcg cggtccgcgt cgttgcttaa atacgcctct
    20221 tcatacatct gcaaatcctg ttgcgttaag ccatcccaga caccgcccaa cagacgacga
    20281 gtcacggaat cccgtaatcc gtaaagccca taccacagca aaacacccgc aattttaccg
    20341 caatcgatct gtttatcacg caaccacaac gcactggcga gcgccagcat ggcacctgcg
    20401 gaatcaccgg caaagccaat gcgggacata ttgatttgat aatcctccgc ctgctggtgg
    20461 aaataacaac aagcagccac aatttcctct atcgcttgcg gaaaacgcgc ttcaggtgaa
    20521 agggggtaat caataccaat caccgtacat tggctgtagc ttgccagcag gcgcatgatg
    20581 cgatcgtggg tatcgagatt gccgagaata aaaccgcctc catgcaaata aaatagcgtc
    20641 gccgggctat ctggctgcgg acaaaagaga cgtgtttcca cctgcccata ttttgttgga
    20701 accatgtaag ctctggttgc catttctgga gcgcccgcat tccagaatcg gcgctcaagc
    20761 gtgtaatact gtcgttgctc agcaatcgtt cccgttgcgg gccaggacgg taaatccggc
    20821 tgaagagtat tcacaacggt cttcatttca gcagaaataa ggtccagaac aggtagtttg
    20881 ttttccggct tcatacaaaa actcctttca aattacgtca ttgtaaggaa ccactgccat
    20941 gaaaatgcga tcccgcctgc tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga
    21001 ttgtgttaac attcgccgct cagttaacca cccgtaaaaa caaccatgaa atttcccggt
    21061 aaacgtaaat ccaaacatta cttccctgta aatgcacgcg atccgctgct tcagcaattc
    21121 cagccagaaa acgaaaccag cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat
    21181 attgaagcga aagtggatga tgaattcatt gagcgttatg gattaagcgc cgggcattca
    21241 ctggtgattg aggatgacgt agccgaagcg ctttatcagg agctaaaaca gaaaaacctg
    21301 attacccatc agtttgcggg tggcaccatt ggtaacacca tgcacaacta ctcggtgctc
    21361 gcggacgacc gttcggtgct gctgggcgtc atgtgcagca atattgaaat tggcagttat
    21421 gcctatcgtt acctgtgtaa cacttccagc cgtaccgatc ttaactatct acaaggcgtg
    21481 gatggcccga ttggtcgttg ctttacgctg attggcgagt ccggggaacg tacctttgct
    21541 atcagtccag gccacatgaa ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc
    21601 ggagcctcgg cactggttct cacctcttat ctggtgcgtt gcaaaccggg tgaacccatg
    21661 ccggaagcaa ccatgaaagc cattgagtac gcgaagaaat ataacgtacc ggtggtgctg
    21721 acgctgggca ccaagtttgt cattgccgag aatccgcagt ggtggcagca attcctcaaa
    21781 gatcacgtct ctatccttgc gatgaacgaa gatgaagccg aagcgttgac cggagaaagc
    21841 gatccgttgt tggcatctga caaggcgcta gactgggtag atctggtgct gtgcaccgcc
    21901 gggccaatcg gcttgtatat ggcgggcttt accgaagacg aagcgaaacg taaaacccag
    21961 catccgctgc tgccgggcgc tatagcggaa ttcaaccagt atgagtttag ccgcgccatg
    22021 cgccacaagg attgccagaa tccgctgcgt gtatattcgc acattgcgcc gtacatgggc
    22081 gggccggaaa aaatcatgaa cactaatgga gcgggggatg gcgcattggc agcgttgctg
    22141 catgacatta ccgccaacag ctaccatcgt agcaacgtac caaactccag caaacataaa
    22201 ttcacctggt taacttattc atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc
    22261 tatcaggtac tgaaccagca ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac
    22321 agcctggaag agtcttactg ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc
    22381 gttcacgccg catctggcat ttggctctcg atgcctgatg cgacgctggc gcgtcttatc
    22441 atgcctacat atttttcata ttttacatcc ggcaaccacc gtttaccccg tcaccacctc
    22501 acccgccggt ggcgtttcca gcagttccag catggtacgg gcgatttcac gctcgcccat
    22561 cactacctga ttcgcaccac gttcggtgat atacgccact tcatcgtcat aatgggcgcg
    22621 ggcaataatc tcaatatccg gatttttcgc gcgggcagat accacaatct cacccgcttc
    22681 ataaccgttg ggaatcgtca ggatcagcca ttttgcacat tccagatgcg ccagttgcat
    22741 aatttcttcg ttcgccgcat tgcccaatac tgcgcggacc ccgcgctctc gcagctcatc
    22801 aacacgggtt cgtgacgtct caatcaccac cagcggaata tcagaggcga gcaatttctc
    22861 ccccagcagg ctgcctacac gaccgtaacc caccagtagc gcatggttgc aaatatccac
    22921 tgggatctgc ttctcttctt cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt
    22981 cttcgccaga tatttctcca gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat
    23041 cgcccctgcc agtaccaggt tttgtccggc ctgcggcagt aaattcaatg ccattcccag
    23101 tcccgccagg ataaacgcga actcaccaat ctgcgccagg ctggcggcga tggttaatgc
    23161 cgtacgttgg gagtgaccaa acagtcgcac caggaaaaat gcggctaacg acttaccaaa
    23221 cagaataatc gccagcgtcg ccagcactgc cagcggttgc tgaatcagaa ttaacggatc
    23281 aaacaacatc ccgacggaga caaaaaacag caccgcaaac gcgtcgcgca atggcagcgt
    23341 atcgtgggcg gcacggtgac tcagttcaga ctcgttcagt accatcccgg caaagaacgc
    23401 accgagtgca aaggagacat caaacagctc taccgcacca aaggcaatcc ctaacgccag
    23461 cgccagcacc gacagggtaa acagctcgcg agaaccggtt gccgcgctgc gtgccataat
    23521 ccacggcacc agacggcgac ctaccagcat cataatggcg ataaatgcga tcactttgcc
    23581 gatggtgatc cccatatcga ctgcaagagt ggcaaagccc acatcgccct gttccatcat
    23641 tcctgcaact gcgggcagca acaccagcgt cagaaccatt accaggtctt ccacaatcaa
    23701 ccaaccgatg gcgatttgcc cacgctgact gtcaattaat tgccgttctt caagtgcgcg
    23761 cagtaacacc acggtactgg cggtggaaag acataaaccg aacacgatac cggtcattaa
    23821 cgaccagccc agcacggcag agagcgccat acccagcagc gtcgccacgg ctatctgggc
    23881 gatcgcaccg ggaatggcga tggcctttac cgccatcaaa tccttcagcg aaaagtgcaa
    23941 accgacgcca aacatcaaca gaatgacgcc cagttcagcc agttccggcg caagcttggt
    24001 atcggcaaca aagcccggag tgaatggtcc tgccagcaca cccgctaaca gatatcccac
    24061 cagaggagaa atacgtagtt tattggccag catgccgagg ataaaggcga gcacaaggcc
    24121 gccaacaatg gtggtgataa gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg
    24181 ttattgtcca tttttggccg ggaaaaccaa aattacaggt aatagtttat gacaatttca
    24241 ttgatgatgt tcatgaataa ttattgaatt ttgcagaaaa atggaattag ctgcaaaaaa
    24301 agcacggatc ggaaaacaga aggcggttta acagaggaaa ggcttatggc gactgtaaaa
    24361 cgatgcagcc aaagtttgcc tttggctgca atgaaatcag tctttatgcc ggttat
//
LOCUS       DAJTNN010000047        24252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTNN010000047 DAJTNN010000000
VERSION     DAJTNN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.047"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(54..764)
                     /locus_tag="OO150_001674"
     CDS             complement(54..764)
                     /locus_tag="OO150_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5515860.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            872..1177
                     /locus_tag="OO150_001675"
     CDS             872..1177
                     /locus_tag="OO150_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5515861.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQSTQDGAPAEPQ"
     gene            1376..1942
                     /locus_tag="OO150_001676"
                     /pseudo
     CDS             1376..1942
                     /locus_tag="OO150_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            1924..2052
                     /locus_tag="OO150_001677"
                     /pseudo
     CDS             1924..2052
                     /locus_tag="OO150_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
     gene            2095..2709
                     /gene="dmsD"
                     /locus_tag="OO150_001678"
     CDS             2095..2709
                     /gene="dmsD"
                     /locus_tag="OO150_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5515862.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQYEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELSRLTLAQWQSQLLIPVAVKPLFR"
     gene            2868..4160
                     /gene="clcB"
                     /locus_tag="OO150_001679"
     CDS             2868..4160
                     /gene="clcB"
                     /locus_tag="OO150_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5515863.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4113..4808)
                     /gene="bioD"
                     /locus_tag="OO150_001680"
     CDS             complement(4113..4808)
                     /gene="bioD"
                     /locus_tag="OO150_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5515864.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4933..6153)
                     /gene="mlc"
                     /locus_tag="OO150_001681"
                     /gene_synonym="dgsA"
     CDS             complement(4933..6153)
                     /gene="mlc"
                     /locus_tag="OO150_001681"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5515865.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNLGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPIISDSIRQQALPTYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLHG"
     gene            complement(6288..7181)
                     /locus_tag="OO150_001682"
     CDS             complement(6288..7181)
                     /locus_tag="OO150_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5515866.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRAQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            7288..8540
                     /locus_tag="OO150_001683"
                     /pseudo
     CDS             7288..8540
                     /locus_tag="OO150_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            8937..9251
                     /locus_tag="OO150_001684"
     CDS             8937..9251
                     /locus_tag="OO150_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5515867.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTTKAAPAKT
                     THHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQP
                     AA"
     gene            9344..9427
                     /locus_tag="OO150_001685"
     CDS             9344..9427
                     /locus_tag="OO150_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515868.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            9527..10348
                     /locus_tag="OO150_001686"
     CDS             9527..10348
                     /locus_tag="OO150_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5515869.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(10387..10716)
                     /gene="mdtI"
                     /locus_tag="OO150_001687"
     CDS             complement(10387..10716)
                     /gene="mdtI"
                     /locus_tag="OO150_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5515870.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(10703..11068)
                     /gene="mdtJ"
                     /locus_tag="OO150_001688"
     CDS             complement(10703..11068)
                     /gene="mdtJ"
                     /locus_tag="OO150_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5515871.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            11480..12514
                     /gene="tqsA"
                     /locus_tag="OO150_001689"
     CDS             11480..12514
                     /gene="tqsA"
                     /locus_tag="OO150_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5515872.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(12539..13927)
                     /gene="pntB"
                     /locus_tag="OO150_001690"
     CDS             complement(12539..13927)
                     /gene="pntB"
                     /locus_tag="OO150_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5515873.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQDVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(13938..15470)
                     /gene="pntA"
                     /locus_tag="OO150_001691"
     CDS             complement(13938..15470)
                     /gene="pntA"
                     /locus_tag="OO150_001691"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5515874.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            15994..16938
                     /locus_tag="OO150_001692"
     CDS             15994..16938
                     /locus_tag="OO150_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5515875.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            17124..18506
                     /locus_tag="OO150_001693"
     CDS             17124..18506
                     /locus_tag="OO150_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5515876.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            18543..19265
                     /gene="folM"
                     /locus_tag="OO150_001694"
     CDS             18543..19265
                     /gene="folM"
                     /locus_tag="OO150_001694"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5515877.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKLGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(19262..19574)
                     /locus_tag="OO150_001695"
                     /pseudo
     CDS             complement(19262..19574)
                     /locus_tag="OO150_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
     gene            19700..20419
                     /gene="rstA"
                     /locus_tag="OO150_001696"
     CDS             19700..20419
                     /gene="rstA"
                     /locus_tag="OO150_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5515878.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGITICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            20423..21724
                     /gene="rstB"
                     /locus_tag="OO150_001697"
     CDS             20423..21724
                     /gene="rstB"
                     /locus_tag="OO150_001697"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5515879.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            21800..22729
                     /gene="tus"
                     /locus_tag="OO150_001698"
     CDS             21800..22729
                     /gene="tus"
                     /locus_tag="OO150_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5515880.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHCDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(22726..24129)
                     /gene="fumC"
                     /locus_tag="OO150_001699"
     CDS             complement(22726..24129)
                     /gene="fumC"
                     /locus_tag="OO150_001699"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5515881.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         5842 a         6263 c         5926 g         6221 t
ORIGIN      
        1 gcagagcaca aaacaccggg tcagactctc ctgaagccga ccgcacaata gcattaataa
       61 taatcgatcg tatttttgat ggtgtaaacc cgttcgacgg cgggttttac tccttcatca
      121 acaatgatca gctgacagtc caccggattg gcgtgactgt catattcaca gctctgtttt
      181 acattaccaa ctcgttgatt attcagtaaa gtaaccgctg tgtaatctaa ttttttgatc
      241 gggtccgttg atggcgtggc gctgacagat aatgttttgt cgttactttt cgtggtttta
      301 cccagtggat aaccctgatc atcatagcga tattccatct gcatttgttt gctgctggct
      361 ttaatcacga agccattatc atcggtttcc caactcaccc cggcagaagg taattctgct
      421 agctggcatt ttccctgtaa acgtactctc ttctccagcg tctcggcatc acggtaataa
      481 ttggcgtcca gtaccagagc gaccacggta ttattttcca gatccagtaa ttcgagtgaa
      541 tcaaaacagc cttcttccga caaagtccca gaaacacgtt tcgtcacttc accttgctca
      601 tccattaatg tctgagtgaa atcttttacc ggaccacgca gcggatcaaa atcgaattca
      661 ttagagaaac tggccatctc aggggtaaat gaaagtgtta cctctgtgcg gtcacatcct
      721 gtgaggaata tcgcgagtaa gcatggtagt aatttgtatt tcacaacagt caccagagag
      781 tagagatgat tctcaatcat agtagcaaat acagtacttt acacgttaaa tgctatgctt
      841 aaagaagtta tcttcgcgta aggagcttat gatgaaactc tcaacctgct gtgccgcgct
      901 tctgctcgcc ctcgcctcac ccgcggtact cgccgcgccg ggatcctgtg agcgcataca
      961 gagcgatata tcacagcgca ttatcaataa tggcgtaccc gaaagcagct tcacattaag
     1021 tattgtaccc aatgaccagg ttgatcagcc tgattcccag gtcgtcgggc attgtgctaa
     1081 tgatacgcat aaaattctct acacccgcac caccagcgct aacgtctctg ctcccgcgca
     1141 gtctacccag gatggcgcgc ctgccgaacc gcagtaatac attatcgact gaacgccgga
     1201 tatgacacat ccggcgattt gaacatacaa cataatccca ccttattact catacccttc
     1261 tattgatgtg gattaataat tcttaaccca aaatgggtag accccctcta ttgttagcgc
     1321 gctaaatatt caatacataa acatttatat aacgataaag aacagggagt gagttatgtc
     1381 caaaaatgac cgaatggtgg gcatcagccg cagaacactc gttaaatcaa ccgcgatagg
     1441 ttctctggcg ctggctgcag gcggtttttc tttaccgttc accctgcgca gtgcagcggc
     1501 aacggtacaa caggccagcg aaaaagtgat ctggggtgcc tgttccgtca actgtggtag
     1561 ccgctgtgca cttcgtctac atgttaaaga taatgaagtg acctgggtgg aaactgacaa
     1621 taccggcagc gatgagtacg gcaaccatca ggtacgcgcc tgtttgcgcg gtcgctccat
     1681 ccgccggcgt attaatcatc ccgatcgctt gaattaccca atgaaacgcg tgggtaaacg
     1741 cggtgaaggc aaattcgaac ggattagctg ggatgaagcc ctggatacta tcgccagtag
     1801 cctgaaaaaa accgtcgaac aatatggcaa tgaggctgta tatattcagt actcttcggg
     1861 gattgttggc ggcaatatga cccgctcttc gccatcagcc tcggcggtca aacgcctgat
     1921 gaactgctac ggcggttccc tcacgttgcc ggacttattc tggggctaat cctcatactt
     1981 ggcggtgaga tgatcgggcg tgtgctcttt tatggcctgc acatgacagt aggaatggct
     2041 attgcaggtt aacaaattgg tgcggggcaa cccgcacatt tcaggatgtt aggaatgacc
     2101 catttttcac agcaagataa tttttctgtc gcggcgcgcg tgttgggcgc gctgttttat
     2161 tacgctccag agagtgcgga agccgcacct ctggttgcgg tactcaccag tgacggctgg
     2221 gaaactcagt ggcctctacc agaagcgtca ttagcgccgc tggtgacggc ttttcagaca
     2281 cagtgtgaag agactcacgc ccaggcctgg cagcgtttgt ttgtcggccc gtgggcactg
     2341 ccgtctccgc cgtgggggtc ggtctggctg gatcgcgaat ctgtgctgtt tggcgattca
     2401 acattggcac ttcgtcagtg gatgcgcgag aaaggcattc agtttgaaat gaagcaatac
     2461 gaaccggaag atcattttgg ttcactgctg ttgatggctg cgtggttggc ggagaatggt
     2521 cgccagacag aatgcgaaga attgctggca tggcaccttt ttccgtggtc aacacgtttt
     2581 cttgatgttt ttatcgaaaa agcagaacac cctttctacc gtgcactggg tgaactgtct
     2641 cgcttaacgc tggcgcaatg gcaatcacaa ctgttaattc ctgtcgcggt taaaccgctg
     2701 tttcgatagg atcaccgtaa tattgccgga tggtgacgat tacgcgcaat ccggcaataa
     2761 taggttacag tgtcacgttt ttttatctct taaagcacgc actgcttttg cggctggcct
     2821 cttttgccgc aaaatagtcg cccgtgtttc attgcccatt tctgctcatg catcatctac
     2881 acatctatcc ggatctgcgc actatgttcc gccgtctgct tatcgcaaca gtcgtcggta
     2941 ttctcgcggc ctttgccgtt gccgggtttc gtcatgcgat gctgctactg gagtggttgt
     3001 tcctcaataa tgactccggc agtctggtca atgcagcgac aaacctttcc ccctggcgac
     3061 ggttgctaac tccggcgctc ggcggactgg cggcgggttt gttgctgatg ggctggcaga
     3121 aatttaccca acaacgccct catgcgccga ccgattacat ggaagcgttg caaaccgatg
     3181 gacagttcga ttacgcagca agcctggtta aatcgcttgc ctcgctgctg gtagtaacca
     3241 gcggcagtgc aattggtcgc gaaggtgcga tgattctttt agctgccctt gccgcctcct
     3301 gttttgccca acgttttacg ccacgccagg agtggaaatt atggatcgcc tgtggcgccg
     3361 cggcgggaat ggctgcggcc tatcgtgccc cgcttgctgg cagtttattt atagccgaag
     3421 tgctgtttgg cactatgatg ttggcctctc tcggcccggt gattatttcc gccgtcgtgg
     3481 cattgctggt tagcaatctg attaatcata gcgacgcgtt actctacaac gtacaactct
     3541 cagtgacggt tcaggctcgt gactatgcgc tgattatcag tacaggtgtg ctggcaggtc
     3601 tgtgcggacc actgttgtta acgttaatga acgcctgtca tcgtggattt gtgagtctca
     3661 aacttgcgcc gccctggcaa ctggcactag gcgggttgat cgtgggtctg ctttccctgt
     3721 tcacacctgc agtgtggggc aacggctata gcaccgtaca atccttttta accgccccac
     3781 cactgttaat gatcattgcc gggatcttcc tttgtaaact gtgtgccgtg ctggcgagta
     3841 gcggttccgg cgcacccggt ggtgtcttta caccgacgct atttatcggt cttgccattg
     3901 gcatgttgta tggtcgtagc ctgggattat ggttccctga tggcgaagaa attacgcttt
     3961 tactcggatt gaccgagatg gcgacactgt tggcggcaac cacgcacgcg ccgattatgt
     4021 cgacgttgat gatatgtgaa atgaccgggg agtatcagct actccccggt ttattgattg
     4081 cctgcgtcat tgcgtcggta atttcgcgga cgttacaccg tgactctatc taccgccagc
     4141 acactgcgca gcatagctaa gcggatgtat tgccccagtt ctcgctgttc ggcgcgcggc
     4201 agataaggca gttcaccaat gagcggtgcc ggaagttttt tacccagcac atcaatgatt
     4261 tccgcataat gcgccagtcc tgggttgatt cggttagcca cccagccaat gagcggcagc
     4321 ccgtcgttgg caatcgcctg agctgttagc agtgcatggt taatgcaacc ttcctgaata
     4381 ccgacaacca tcaacaccgg caactgttcc tgcaccaccc attcagagag tggacgcaaa
     4441 tcattcatca gactgcgcca gccgccagtc ccttctacca cgacatgatc gactttatcg
     4501 gtcaggtttg ccaggccgtt tgaaatgagg gtgtaattga ttgggcaact gtgcgccacg
     4561 ctactttctt cttcgcttaa cgcgatagga ttaactgctt cataaggtag ttcgatggtt
     4621 gaaacactct gcaacaccag ggcatcttta ttacgcagcc cttcgggtgt ctctttgctc
     4681 cccttcgcta cgggtttata tcccgcaact gtttttccct gggaggctaa cgcttgtagc
     4741 aatgcgcggg aaaccaccgt tttccctaca gaagtgtctg taccggtaat aaagaaacgc
     4801 ttcagcatca ctaactccac cgttatgctt cacaaatata aactaggaaa ataattaacc
     4861 ttgaaagtct aagttatgct ttcctggccc aaattgagat agcgcaaatt ttggtagaac
     4921 agttaaaaaa tgttaaccat gcaacagacg aatcaacaaa gaaccgttat acatcgcgtc
     4981 ttttaccagc gcagcgcctg ccatcgtgcc ctggttagaa aactgagtac tctcaacgct
     5041 aatgtgctga ctatacgtag gaagggcctg ctgacgtatg ctgtccgaga tgatagggaa
     5101 gaggatatct gccgctttac ttaacggtga gccaatcagt attttttgtg ggttaaataa
     5161 attcaccatg atggcaagaa tgcgcccgac atgcgcgccc accccggtaa tgatgtcttt
     5221 tgccagtaga tcgccgcgca atgccgcctg acacaatgag tccacggtta acggttgccc
     5281 atgtaacatc gagctcatgg attgattgag acgcagctgt gccagctcaa gaatactgtc
     5341 cacactggcg atggtttcga ggcagccgag gttcccgcaa taacagcgtt taccatatgg
     5401 gtcgacctgt gtgtggccta tttccacgag actactgctg cctgcgtgta gcagatgacc
     5461 atcggtaatg acgcccgccc ccacgttgtg atcgataacc acctgaatca catcgcgcgc
     5521 cccgcgtgag gcaccaaaca aggcctctgc catcgtccat gcgctgatat catgctgaat
     5581 ataaaccggt acgccggtat gctgctccag cgcctcgccg agcggcatct cttttacatc
     5641 ctcgtagaac ggcatgcgat gtacaatacc attttccgta tcaataattc ccggcagggt
     5701 tatggcaatc gaagttagac gctcaagttt tttctggtgg cggataaaaa actgatcgat
     5761 atgggaaatg atacgatcca gcaatggcaa gtcatctttt aacgccagtt cctgcgactc
     5821 ttccaccacc agtttgctgc tcagatcgcg cagagcaagg aaaatctcac cgcgactaat
     5881 gcgcagagaa agatagtgcc aggcttcagt ttcaaccacc agtcccaccg ccggacggcc
     5941 acggttcccc gcttctttga tttccagctc ttgcaccaag tgtgcttcga gcatctcacg
     6001 gacaatttta gtgatactgg caggagccag ctgcgccaga cgggaaagat cgatacgcga
     6061 gactggacca agctgatcaa tcaggcgata aaccgcgccc gcgttggtct gctttatttg
     6121 atcaatgtgc ccaggctggt tttcagcaac caccgcatac tccctatatt ttcgcgctcc
     6181 gaaataatct gtaggctatg gtgaagcact tcaatacgtg tcgtcaaatt tttacttaga
     6241 catgtgatta acagcacatt tttcgggctt tttcgctgaa atttccctca cctgagagca
     6301 ttcagcagat gaatacgaaa gttacgcgaa gctggacttt gttcatgatg tttcggccag
     6361 accaaccaca tttcagaaac cgcatcctct tcagcaatcg gcacccagcg catttcgttg
     6421 agctgagcac gtttaaatga agcaggcaaa attgaaacac ccagaccggc ggaaaccaga
     6481 ccgatgatgg tcattgcctc gcccacctcc tgagtgatga cgggcgtcaa atggtaacgt
     6541 cgcatcagcc cgagaatatc gtcatacagc cctgtcccga cgtgcggatc aaaaaagacg
     6601 aagggttctt tcgccagttc agccaacgtt acattcgggt tatttgccag gggatgatcg
     6661 tgcgggatca tcgccataag cggttcatgg acgatgactg cgtgttcaag cgtctccggt
     6721 aacgctgtgt tacgcagcaa tcccatatcc agcgttcctt caatgagcgg agcgatttgc
     6781 tcacgagtgt tcatttcgcg ggtttgtaaa tggacaccgg gataatcacg gcgaaacagc
     6841 gataacgtat cggacaccgc ccgaataaaa ggagcggacg aagtaaaacc aatgcgcaac
     6901 tcccccgctt caccctgatg cagtctttca gcgcgagcgg cagcgtcatc taccatagac
     6961 aggatttgcc gactatctgc aagaaactgt tttcctgctg ccgtcagcaa tacactgcga
     7021 ttagttcgtg ccagcagtcg ggcaccaatt tgttgctcca gtgcctgaat ctgctgactt
     7081 agcggtggtt gcgaaatatt caggcgggca gcggcgcgcc cgaaatgcag ctcttccgca
     7141 acagcaacaa agtaacgcag atgacgaagt tcaatattca tatttaaaac atcttatttg
     7201 agattattaa tatattagac agaacaattc gattttccta acctatgtat aagcctgatc
     7261 cacaggcata tttagcaagg atttcaagtg agccgtacta caactgttga tggcgctccg
     7321 gcaagcgaca ctgacaagca aagcatttct cagccaaatc aatttattaa acgcggtacg
     7381 ccgcaattta tgcgcgtcac cctggcgctg ttctctgccg gactggcaac ctttgcactc
     7441 ctctattgtg tgcagcctat ccttccggtg ctttcgcagg agtttggctt aacccccgcg
     7501 aacagtagta tttcactgtc catttccacg gcgatgttgg ctattggttt gctgtttact
     7561 ggccccctat ccgatgccat tggtcgcaaa ccagtgatgg tcacggcgct actgttggcc
     7621 tcaatttgta cgttatttcg acaatgatga ccagctggca cggcattttg attatgcgcg
     7681 ccttgattgg gctttcgtta agtggcgtgg cagctgttgg catgacttat cttagcgagg
     7741 aaatccaccc cagtttcgtg gccttttcga tggggttgta tatcagcggc aactcaattg
     7801 gcggcatgag cggacgctta attagtggtg tcttcacgga ctttttcaac tggcgaattg
     7861 ctctggcggc aatcggttgt ttcgcgctag cctcggcgtt gatgttctgg aaaatccttc
     7921 cggaatcacg ccattttcgc ccgacttcgc tgcgacctaa gacgttgttt atcaactttc
     7981 gtctgcactg gcgtgaccgg ggattaccgt tattgttcgc agaaggcttt ttgctgatgg
     8041 ggtcgttcgt cacgctgttt aattacatcg gctatcggtt gatgctttcc ccctggcatg
     8101 tcagtcaggc cgtggttggc ttattatcgc tggcttattt gaccggtaca cggagctcac
     8161 ccaaagccgg aaccatgacc acccgctacg ggcgcggtcc ggtgatgttg ttttcgacgg
     8221 gggttatgct gtttggttta ctgatgacct tattcagctc gctgtggctg atctttgccg
     8281 gaatgttact cttctcagca ggattcttcg cagcccactc agttgccagc agctggatcg
     8341 gcccccgcgc aaaacgcgct aaaggccagg cctcctcgct gtatctgttc agttactatc
     8401 tgggatcgag tattgccggg acgctgggtg atgttttctg gcataactat ggctggaacg
     8461 gcgtcggcgc atttattgct ctgatgctgg tcattgctct gctggtcggg acgcgtttgc
     8521 atcgtcgtct gcacgcctga aaaataagcc cggactgcgg taactacacg tccggactta
     8581 ttgcttgctc aaaccaacgt taatagccat cctaaaatag acgaagcgcc agccaattcc
     8641 cgcagtgcgt ctagcgtcat caggattata agtacccaaa taaacggatt cattttgctg
     8701 tgtgtcattt attactgatg cgcagttatt ctaccgcttt gtaagtagta aaatagttaa
     8761 cccgatcaag actactatta ttggtagcta aatttccctt aagtcacaat acgttattat
     8821 caatgctgta atttattcag cgtttgtaca tatcgttaca cgctgaaacc aaccactcac
     8881 ggaagtctgc cattcccagg gatatagtta tttcaacggc cccgcagtgg ggttaaatga
     8941 aaaaacaaat tgagggtatg acaatgaaaa aagtattagc tctggttgtt gccgctgcta
     9001 tgggtctgtc ttctgccgcc tttgctgcag agactgcgac gaccaccaaa gcagcgccgg
     9061 cgaaaacgac acatcataaa aaacagcata aagcagcacc tgcccagaaa gcgcaggcag
     9121 ctaaaaagca tcataaaaat acgaaagctg aacagaaagc ccctgaacaa aaagcgcagg
     9181 cagcgaagaa acacgccggg aaacacagtc atcagcaacc ggcaaaacct gctgcacaac
     9241 ccgcagcgta agttttcaac agtaatgctg gcgcgccccc tcgcgcctga aaattacggt
     9301 gctaagcggg taacgtttag caccgccttt agcggagggt agtatggtgg gccgttatcg
     9361 ctttgagttc attcttatca tccttatttt atgcgcactg attgccgccc gtttttatct
     9421 ttcctgactg tagttatctg attttactcc cactttcatc ccgtcccgtc tatagtattt
     9481 atgagggttt gcttttaata atcataatta cccaccagag tgtgatatgc gtacaaccat
     9541 tgctgtagtg ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag ataaaccaga
     9601 cgttgccaaa tcggcaaacg atgaggtcag caccctgttt tttggtcatg atgatcgtgt
     9661 gccagtgaat gacacggccc aatcaccgtg ggatgcggtt gggcaactgg aaacggccag
     9721 cggcaattta tgtacggcga cgctgattgc acccaatctg gcattaacgg caggacactg
     9781 tttattgaca cctccaaagg gtaaagccga taaagcagtg gtgctgcgtt ttgtgtcaaa
     9841 taaaggtctt tggcgctatg agatccacga catagaaggc cgcgttgatc cgacactggg
     9901 aaagcggtta aaagcagatg gggatggttg gattgtacct cccgcagccg cgccgtggga
     9961 cttcggattg attgtgctac gtaatccccc ttctggcatt acaccgttgc cgttatttga
    10021 gggagataaa gccgcgctta ctgccgcatt aaaatcggct ggccgtaaag taactcaggc
    10081 gggataccct gaagatcatc tcgatacgtt gtacagtcat caaaactgtg aagtgactgg
    10141 ctgggcgcaa acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg acagcggttc
    10201 gcctctgatg ttgcatacca atgacggctg gcaattaatt ggggtgcaaa gttcggctcc
    10261 tgccgcgaaa gatcgctggc gcgccgataa ccgggccatt tctgttaccg gttttcgcga
    10321 caagctggat caactgtcgc aaaaataatg ttcaaacgct gcccgactgt gcgggcagcg
    10381 tgttcatcag gcaagtttca ccatgatcat tccagccaac agcaagacca ggccaatcca
    10441 gcctttacga tttaaccgtt gaccaaacaa gatccaacct gcggctaacg tggcggcaat
    10501 accaaacccg ccccacaatg cataagcgac agacaagtcg atccctttaa cggcctgaga
    10561 aagcgcacta aaggcagcca gcaccgccgc cagggagagc aagccaaata ttttgcgacg
    10621 aaagccgtca gaaaatttca aaaagacgtt agcaacgatt tccagcacga ttgccaatgc
    10681 cagccaggcg gcgtgaaccc attcaaactg cgccatggtt tacctccagt tcaggtttac
    10741 gcgctttacg ggtacctgat tttatcaaca caatcccggc gaccagggtg gttaacccgg
    10801 caattttcat cagcgataaa ctttcgtcga ataacaaaac gctaaacaag gtaataaata
    10861 aaataccgat accttcccac aacgcataag ctacgcctaa ggcgatcttt ttaacggcga
    10921 aagagagaaa tatatacgac agagaaatca tcaccagcat taaaataaag ccgccatttc
    10981 cctcactgac gctcgcccat ttcattgaca gcgtaccggt aatttctgta gcaatagcca
    11041 ggcctaataa aatccaataa atatacattg tccttctcct gcaagagaat tattttaatt
    11101 ttcgcttaat tcagcgaaat caaagtaaag tggtcaagcc ccgcgcatag ggcaagctta
    11161 ggcagaagaa aggactaaag cgcgttgcgc cagtgctgct cacctacgag caagatagtg
    11221 gatgaggtac aaagatgggt aaatgttgaa aataaggtcc tgaacaaatt gtccataata
    11281 ttacaattat ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc tcggtagttg
    11341 aacacgcctg atttgtatca tagcttaaga attaactcaa aatattttca cttctttacc
    11401 tgagcggttt gattttcgtt atgatgacgg agcgaaaaag acattattat tagcaaagga
    11461 agaaaaaacg gggacaagca tggcaaagcc gatcatcacg ctcaatggcc taaaaatcgt
    11521 cattatgttg ggaatgctgg tcattattct ctgcggtatc cgttttgccg ccgagatcat
    11581 cgtgccgttt attctcgcat tatttattgc tgttattctt aacccgctgg tgcaacacat
    11641 ggtccgctgg cgagtgccgc gtgtactggc ggtgtcgatt ttgatgacca tcatcgtgat
    11701 ggcgatggtg ttgctattag cttatctggg ttccgcgctc aacgagttga cgcggacgtt
    11761 accgcaatat cgcaactcta ttatgacgcc gctgcaagct cttgagccgt tgttgcaacg
    11821 cgtagggatt gacgtctcag ttgaccagct ggcgcattac attgatccga acgcagcgat
    11881 gacgttgctc accaacttat tgacgcagtt atctaatgcc atgtcatcaa tatttttatt
    11941 gctgctgacg gtgctgttta tgctgctcga agtgccacaa ttgcccggaa aatttcagca
    12001 aatgatggcg cgtccggttg aagggatggc ggcgattcaa cgtgcaattg acagtgtttc
    12061 tcattatctg gtgctgaaaa cagccatcag catcatcacc ggcctggtcg cctgggcgat
    12121 gctcgccgca ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa
    12181 ttacatccct aatattggtt cagttctcgc ggcaatcccc cctatcgctc aggtactggt
    12241 gtttaatggc ttctacgaag cgttgctggt gctggcggga tatctgctga ttaatctggt
    12301 cttcggcaat attctggagc cgcgcatcat gggacgtggg ctggggcttt ccacattggt
    12361 ggtatttttg tcgttgattt tttggggatg gttgttagga ccggtgggta tgctgctttc
    12421 cgtgccgtta acaattattg tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat
    12481 cgccgttctg ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc cgtcagggtt
    12541 acagagcttt caggattgca tccacgctgg ctttggcgtc accaaacagc atgtgggtgt
    12601 tttccttgaa gaacagcggg ttttgcacac cagcatagcc agtgttcatc gaacgtttaa
    12661 agacaatcac gtcctgcgct ttccacactt ccagcacagg cataccagca ataggactct
    12721 tcggatcatc ctgcgccgcc gggttaaccg tatcgttagc accaatcacc agtacggtat
    12781 cggtatcagc aaagtcatcg ttgatctcgt ccatttccag cacgatgtca tacggtactt
    12841 ttgcttcagc cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc gggtggatac
    12901 cgaaacgcac gttgatacca cgagcgcgca gtttctcggt aatttcagcg acaggatatt
    12961 gcgcctgcgc gactgccatg ccgtaccccg gagtaatgat cactgaatgg gagtttttca
    13021 gcagttccgc tgtctcttct gcggtgattt cgcggtgctc gcccacttcc tgatcatcgc
    13081 cagtagaaga gccgtcggtg ccgaaaccac ccgcaataac gctgataaag gaacggttca
    13141 tcgccttaca cataatgtaa gaaaggatag cccccgaaga accgaccagc gcaccggtca
    13201 caatcagcag gtcgttgctg agcataaagc ccgcagccgc agccgcccag ccggagtacg
    13261 agttcagcat cgacaccacc actggcatat ctgcaccacc gatggaggcg actaaatgcc
    13321 agccgaatac cagcgcaatg gcggtcatta ttagcaatgc cagcacttgc aggccgacgc
    13381 tgtccgtgcg aacaaataca atcagcagca ggaaggaaac gaccagagcc gccaggttca
    13441 ttttgtgacg gtttggcagc atcaacggtt tagacgaaat cttgccacac agtttgccga
    13501 acgccaccac cgaacccgtg aacgttaccg ccccgatgaa gataccgagg aacacttccg
    13561 tcaggtgaat attgaccaga atcggtgcca ttcccgcgtc atgatgcaga tagctgttaa
    13621 agccaaccag cactgccgcc agacccacga agctgtgcag gatcgccacc agttctggca
    13681 tttcggtcat ttcaactttc ttcgccagac ggataccaat tgccccacca atgaccatcg
    13741 ccagcaagat ccagccaaca ttacccgtat ccggtccaaa aatggttgcg attaacgcaa
    13801 tcgccatccc tgcgataccg aagttgttac cctggcgaga cgtttcatgt ttcgaaagac
    13861 cggccagact gaagataaac aggatcgcgg caacaatgta tgcagctgta actaatcctc
    13921 cagacatatg ttacccctta atttttgcgg aacattttca gcatgcgctg agtcacggtg
    13981 aagccaccga aaatattaat gctggctata agcaccgcga taaaactaag gaagctaacc
    14041 cagccgccct ggccaatctg caacagtgct ccgacaacaa taatccctga aatcgcgttg
    14101 gtgaccgaca tcaacggtgt atgcagcgcg tgcgatacat tccacaccac gtaataaccg
    14161 acaacgcagg ccagcgcgaa aacggtgaag tgcccaagaa actctttcgg cgcaacgctt
    14221 gccagccaac cgaaaaggat gattgccagc gccatcaacg cgtatttacg ccacggtgag
    14281 caggcacatt tttcctcagt tttcacttcc ggtgccgctt tttgtgccgc ctgcggctga
    14341 gctgatacct gaatcggcgg tgccggccag gtaatttcgc ccgcacggat cacggtcacg
    14401 cggcgaatca ccacatcatc aaaatcaaca gtgatattgc cgtctttctc tttgcacaac
    14461 agtttcagca gattaacgag gttagtaccg taaagctgcg aggattgcgt cggcagacgg
    14521 cccggaagat cggtataacc aatcactttg acaccatttt ccgtagtgaa gatttcaccc
    14581 ggcacggtgt attcacagtt gccaccgttt tgggctgcca ggtcgacaat cacactgccc
    14641 gtcttcatgg agtcaaccat ttcacgggta attagcttcg gcgctggttt gcctggaata
    14701 agcgcggtgg tgacaatgat atcgacctct tttgcctggg cggcaaagag ttccatttct
    14761 gctttaatga acgcgtccga catcactttg gcatagccat cgccgctgcc cgcttcctct
    14821 ttaaaatcca gctcgaggaa ttccgcgccc atactttgaa cttgttcttt cacttccgga
    14881 cgggtgtcga atgcacgcac aatcgcgccg agactgtttg ctgcgccaat ggcggccaga
    14941 cctgcaacac ccgcaccaat caccatcact tttgccggtg gcactttccc ggccgcagta
    15001 atttgcccgg taaagaagcg cccaaattca tgtgccgctt caacaatggc gcgataaccg
    15061 gcgatgttcg ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca
    15121 gaatccatcg ccatcacggt cacgttacgt tccgcaagtt tttgcattaa ttccggattc
    15181 tgcgcaggcc agataaaact caccagcgtt gtcccaggat tcagtaacgc aatttcatca
    15241 tctaacggcg cattgacctt cagaatgatc tctgactgcc agacgctatt cccttctaca
    15301 atttcagcgc ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg
    15361 ctctctaccg cgacggtaaa acccagtttc agcagctgtt ccactgtttt tggcgttgct
    15421 gcaacacggg tttcattggt taaccgttct cttggtatgc caattcgcat gatattccct
    15481 tccatcggtt ttattgatga tggtttgcct gtgtcaggag ccacacaagc cgctcatgta
    15541 cgagctaaat gttactccgt taaaataaat tagtaacaaa cgccctataa cgtactgaaa
    15601 attatgcctg tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa
    15661 cgattcagcc gtgatagtgg gataaacacc ttagaacgcc ggataaagac tgataattgt
    15721 cttcgacggt cgggtaaaac gagacatcgc cccggcacga ttcactactt aacattaaat
    15781 taacttatac aattcagttg cttcagtagt aatgatgctg atacggctgt tttttaagca
    15841 tagacggtca tttgagcagg attaaaattg gcttaaggaa tgtgatatga aaaatgacgc
    15901 agacagttac accgtttaaa tgcaataatc agccacgttt ctcgttaata acaataccag
    15961 tacctggttt gcgcaaggcg aaggattatt tttatgaagc ttaagaacac cctcctggcg
    16021 tcggcactgc tttctgctac ggcattctcc gttaacgcag caacagaact gacaccggag
    16081 caagcggcag cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt taatgctatt
    16141 ggcgaagcgg tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc ctctttttat
    16201 gttgtcgaca cttctgattt tggtaacagc ggtaactggc gtgtggtcgc tgacctctat
    16261 aaagccgatg ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa
    16321 ctgccgaaag atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc
    16381 tatcgtagcc agccagaagt caatgatgcc atcaccaaag cggcaaaagc gaaaggtgcc
    16441 tactctttct acatcgttcg tcaaatcgat gccaaccagg gcggcaacca gcgtattact
    16501 gcattcatct ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg
    16561 gcagattccg aagcaggacg tgcagctctg gctgccggtg gcgaagccgc gaagaaagtt
    16621 gagatcccag gtgttgcgac taccgcatca ccaagttctg aagtcggtcg tttctttgaa
    16681 acccagtcat caaaaggcgg gcgttacacc gtcacgctcc cggatggcac taaagtcgaa
    16741 gaactgaaca aagcgaccgc agcgatgatg gtcccgttcg atagcatcaa attctctggc
    16801 aactacggca acatgaccga agtctcctat caggttgcga aacgtgccgc gaagaaaggt
    16861 gctaagtatt accacatcac ccgccagtgg caggaacgtg gtaataacct gaccgtcagc
    16921 gcagatctgt ataaataact attcagttac tcctataagg cggcttgatg ccgcctttcg
    16981 catttttagt cacaaatttc cccgcgacca ttgcatgcag acacatctct tcgtaaaatc
    17041 ccgcgctcca gtggcaaact ccatttttat gcgttttgcc ctaatattta ttcttcttat
    17101 cacgttttaa tcactggata tcgatggaaa agaaactggg actgagcgca ctcaccgcgc
    17161 tggtattaag ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat atggcggcag
    17221 ttgccagccc ggcagcactg ctcatcggct ggggtattac tggcgctggc attttattgc
    17281 tggcctttgc catgctgatc ctcacgcgca ttcgtcccga acttgacggc ggtatcttta
    17341 cctatgcccg cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg ggatactggc
    17401 tgtgcgcagt catcgccaac gtctcctatc tggtgatcgt tttttccgcg ttaagctttt
    17461 ttacggacac gccggaattg cgcctgttcg gcgatggtaa tacctggcaa tcgatcgtcg
    17521 gtgcatcggc tttattgtgg attgttcact ttttgatcct gcgcggggtg caaaccgctg
    17581 ccagcattaa cctggtggcg acactggcaa aattgttgcc gctgggtctg tttgttgtgc
    17641 tggcgatgat gatgttcaaa ctggatacct tcaagctcga cttcaccgga cttgtacttg
    17701 gcgtacccgt ttgggaacag gtgaaaaaca ccatgctgat caccctgtgg gttttcattg
    17761 gtgtggaagg tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat gttggcaaag
    17821 cgacactgct ggcggttctc tccgctctgg gcgtttactt gctggtaacg ctgctttcgc
    17881 tgggtgtggt ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg gccggtctga
    17941 tggtggaaat gatgggacca tggggcgaaa tcatcatcgc tgccagtttg atcgtttccg
    18001 tttgcggcgc gtacttaagc tggaccatca tggcggcaga agttccgttc ctggctgcca
    18061 ctcataaagc attcccacgc atttttgcgc gccagaacgc acaagctgcg ccatccgcct
    18121 cactgtggtt gaccaacatc tgcgtgcaaa tttgtctggt actcatctgg ctgaccggtt
    18181 ccgattacaa cacgttgctg accatcgcct cagaaatgat tctggtgccc tatttcctgg
    18241 tcggcgcgtt cttgctgaaa atcgcaaccc gtccactgca taaagcggta ggtgtcggtg
    18301 cctgcattta tggcttatgg ttattgtacg cctctggtcc aatgcacttg ctgttatccg
    18361 ttgttctgta tgcgccgggg ctgctggttt tcctgtatgc gcgcaaaacg catacacatg
    18421 ataatgtact gaaccgtcag gaaatggtgc tgattggcat gctgttaatt gcctcagttc
    18481 cggcgacctg gatgctggtg ggataacgtt catcccatcg ttggatagca aaggagataa
    18541 cgatgggtaa agcccagccc ttgccaatat taattactgg cggaggtcgt cgcatcggcc
    18601 tcgccctcgc ctggcatttc attaatcaaa agcaaccggt gattgtcagc tatcggacgc
    18661 actatccagc cattgatgga ctgattaagg caggtgcgca gtgtattcag gctgattttt
    18721 cgaccaacga cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc catggtctgc
    18781 gtgctatttt gcataacgcc agtgcgtgga tggcggaaaa actgggtgcg ccactggccg
    18841 acgtactggc ttgcatgatg cagatccacg ttaatacccc atacctgctc aaccatgcgc
    18901 tggaaagatt actgcgtggg cacggacacg ccgccagcga tatcattcac tttaccgatt
    18961 atgtggtgga gcgcggtagc gacaagcata ttgcgtatgc tgcaagcaaa gcggcactgg
    19021 ataatatgac ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg aattctattg
    19081 ctccatcgct gatcctgttt aatgaacatg atgatgccga atatcgacaa caggcgctga
    19141 ataaatcact gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg gtcgattact
    19201 tacttaccag ttgctttgtc accggacgca gtttcccact tgatggcggt cgtcatctgc
    19261 gttaatgcag ctttatccag catataatca acacccatgc aacacgcaac agaaccaaca
    19321 aaagcggcgg gcaagcgcat cattccggtg aaataccaca gcgacaccag gtaaacaaaa
    19381 tagggaataa tcgaccacat actaaaaatg atggttgcgc gtaaggcttc aatgccgcgt
    19441 tcgctggcaa caatataatg cgcgataagc gcaaaggtcg ggaaaagtgg aatcagcccg
    19501 gcgatataat aatttttcgt ttttgctaaa acaccaatca acagcactac cagcgcaccg
    19561 agcgcggctt tgattttacc ttaacacttc cataacaagt catcagtaga atacctgatg
    19621 aaaacttgtt tagaaacgat tgatagtaag taaaaacagc gcggtgtatt gtgacgtttt
    19681 tatatctacc gtgaatgtta tgaacactat cgtatttgtg gaagatgatg cggaagtcgg
    19741 ttcactgatt gccgcgtacc tggcaaaaca tgatatgcag gttaccgtag agccgcgcgg
    19801 cgaccaggcc gaagaaacca ttttgcgaga aaatccggat ttggtgttac tcgacatcat
    19861 gctaccaggc aaggacggca ttaccatttg tcgtgattta cgcgcaaagt ggtctggacc
    19921 gattgttctt ctaacctctc tcgatagcga tatgaaccac atcctggcac tggaaatggg
    19981 tgcctgcgac tatattctca aaacgatgcc ccctgctgtt ttgctagcgc gtttacgttt
    20041 gcatttgcgt cagaatgagc aagccacact gaccaaaggt cttcaggaaa cgtctctgac
    20101 tccctacaaa gccctgcatt tcggcacgtt gaccatcgat cccatcaacc gcgtagtcac
    20161 cctggctaac actgaaatct cgctctcgac agctgatttc gaattattgt gggaattagc
    20221 tacccatgcc gggcaaatca tggaccgcga tgcattgctg aaaaatttac gcggcgtcag
    20281 ttatgacgga ctggatcgta acgtggacgt ggctatttcg cggttaagaa aaaaactgct
    20341 cgataacgcc gcagaacctt atcgcattaa aactgtgcgt aacaaaggct atctttttgc
    20401 gcctcatgca tgggaataag cgatgaaaaa actgtttatc cagttttacc tgttattgtt
    20461 tgtctgcttc cttgtgatgt ctctgctggt tgggttggtg tacaaattta ccgccgaacg
    20521 cgcgggcaaa cagtcgctgg atgatttgat gaacagttcg ctgtatctga tgcgcagcga
    20581 attgcgtgag atccccccac acgactgggg taagacgctg aaagagatgg atttaaatct
    20641 ctctttcgat ctgcgtgtcg agccactgag taaataccat cttgatgata tttccatgca
    20701 ccgactgcgt ggcggcgaaa ttgtcgccct ggacgatcag tacacgtttt tgcagcgtat
    20761 cccgcgcagc cactacgtgc tggcagttgg tcctgttcct tatctttatt atctccatca
    20821 gatgcgattg ctggatatcg ccctgatcgc ttttattgct atttccctcg cctttccggt
    20881 gtttatctgg atgcgtccgc actggcagga tatgttaaaa ctggaagcag cggcgcaacg
    20941 atttggcgat gggcatctct atgagcgtat ccactttgat gagggttcga gctttgaacg
    21001 acttggtatc gcatttaacc agatggcgga caatatcaac gccttgattg ccagcaaaaa
    21061 acagcttatt gacggtatcg ctcacgaact gcgaacaccg ttagtgcgcc tgcgttatcg
    21121 actggagatg agcgataacc tgagcgccgc cgagtcccag gcgttgaatc gtgatatcag
    21181 tcaacttgaa gctttaattg aagagctgct gacttatgcc cgactcgatc gcccacaaaa
    21241 cgagcttcat cttagcgaac cagacctgcc gttgtggctg tcaacgcatc tggcagatat
    21301 tcaggcagta acgcccgata aaacggtacg gataaaaacg ctcgtgcaag gccattatgc
    21361 ggcgttggat atgcgcttaa tggagcgcgt gctggataat ttgctcaata acgccctgcg
    21421 gtactgccat tcaacggttg aaaccagcct gctactgtcg gggaatagag cgacattaat
    21481 tgttgaggat gatggcccag ggattgcccc agaaaaccgc gaacatatct ttgaaccttt
    21541 tgttcgcctc gatcccagcc gggatcgctc aaccggcggc tgcgggctgg ggctggcaat
    21601 tgtccactct atagcactgg caatgggcgg tacggttaat tgtgacacca gcgaactggg
    21661 tggtgctcgc ttctcgttta gctggccgtt atggcataac atcccgcaat ttacctctgc
    21721 ctgacactac gcgcacgatg gtcaagtcac caccattgtg ctataaatta aggatgttgt
    21781 aactaaagtg gttaatatta tggcgcgtta cgatctcgta gaccgactca acactacctt
    21841 tcgccagatg gaacaagagc tggctgcatt tgccgctcat cttgagcaac acaagctatt
    21901 ggttgcccgc gtgttctctt tgccggaggt aaaaaaagag gatgagcata atccgcttaa
    21961 tcgtattgag gtgaaacaac atctcggcaa cgacgcgcag tcgctggcgt tgcgtcattt
    22021 ccgccattta tttattcaac aacagtccga aaatcgcagc agcaaagccg ctgtccgtct
    22081 gcctggcgtg ttgtgttacc aggtcgataa cctttcgcaa gcagcgttgg tcagtcatat
    22141 tcagcacatc aataaactca agaccacgtt cgagcatatc gtcacggttg agtcagaact
    22201 ccccaccgcg gcacgttttg aatgggtgca tcgtcatttg ccggggctga tcacccttaa
    22261 tgcttaccgc tcgctcaccg ttctgcacga ccccgccact ttacgttttg gctgggctaa
    22321 taaacatatc attaaaaatt tgcattgtga tgaagtcctg gcacagctgg aaaaaagcct
    22381 gaaatcacca cgcagtgtcg caccgtggac gcgcgaggag tggcaaagaa aactggagcg
    22441 agagtatcag gatatcgctg ccctgccaca gaacgcgaag ttaaaaatca aacgtccggt
    22501 gaaggtgcag ccgattgccc gcgtctggta caaaggagat caaaaacaag tgcaacacgc
    22561 ctgccctaca ccgctgattg cactgattaa tcgggataat ggtgcgggcg tgccggacgt
    22621 tggtgagttg ttaaattacg atgccgacaa tgtgcagcac cgttataaac ctcaggcgca
    22681 gccgcttcgt ttgatcattc cacggctgca cctgtatgtt gcagattaac gcccggcttt
    22741 catactgccg accatctgtt ctggccgtac ccagctgtca aactcggctt cgctaagata
    22801 ccccagcgca agggccgcag cttttaaggt cagcccttct ttatgcgctt ttttggcgat
    22861 ctccgcggct ttgtcataac caatgtgggt gttaagcgca gtcaccagca tcagcgattc
    22921 attgagtaat tgattgattc gctcacgatt cggttcaata cccactgcgc agtgtttgtt
    22981 aaaactttcc atgccatctg ccagcaagcg caccgattgc aggaaattat ggatcaccat
    23041 cggacggaag acgttcagtt caaagttacc ggaagcgcca cccatgttga tcgccacgtc
    23101 gttccccatt acctgacagc agagcatggt taatgcttca cactgggttg ggttcacttt
    23161 ccccggcatg attgagctgc ccggctcatt ttccgggatt gagatttcac caattccgca
    23221 gcgcgggcca gaggccagcc agcggacatc attggcgatt ttcatcagtg acgcagccaa
    23281 ccctttcaac gcgccgtgcg cctgaaccag ggcatcacag gtcgcaagcg cttcaaattt
    23341 gttcggcgcg gtaacaaacg gtgcacaggt aatgactgcc agttcatctg ctacgcgacg
    23401 cgcatactcc ggatgggtat ttagtccagt acccaccgct gtaccgccaa gagccagttc
    23461 cgctacgtga ggcaggctgt attcgatatg tttgagatta tgctcgagca tcgctaccca
    23521 gccggaaatc tcctgcccta gtgttagcgg cgtggcgtcc tgcaagtggg ttcgaccgat
    23581 tttgacgata tcggcaaatg cacgcgattt ttcactcagt gtctgcgtca gggttttaag
    23641 ctgcggaatg agttgcttgc gcagcgccag cagcgccgca acgtgcatcg ccgtcggaaa
    23701 gacatcgttg gaactttggc ttttgttcac gtcgtcgtta gggtgaactt tacgttccat
    23761 cccgcgcacg ccgccgagta attcactggc ccggttagcc agcacttcgt tcatgttcat
    23821 gttactttgc gtgccggagc cggtctgcca gatagccagc gggaattcgt cgtcatgctg
    23881 tcctgccagt acttcatccg ccgcctgccg aatggcgctc gctttctctt cagacaacaa
    23941 gcctaaatct tcattaactt ttgccgctgc gcgcttggtt agcgccagcg catgaatcag
    24001 tgaggtgggc attttctccg tcgaaatgcg gaaatgctcc agcgagcgtt gagtttgtgc
    24061 gccccacagc ttatctgccg ggacatcaat cgcccccatc gaatcttttt cgctgcgtac
    24121 tgtattcatg gcctgctcct cacctgttta attttttctt tctgttttgc tttcgctaag
    24181 caacttttag ctcacttatt atttactatt tgataacaag tgtttggtct ttcgtgccat
    24241 gtaaaaaaac cg
//
LOCUS       DAJTNN010000048        24216 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTNN010000048 DAJTNN010000000
VERSION     DAJTNN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.048"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..2747)
                     /gene="valS"
                     /locus_tag="OO150_001700"
     CDS             complement(<1..2747)
                     /gene="valS"
                     /locus_tag="OO150_001700"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5515882.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVSL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FV"
     gene            complement(2747..3190)
                     /gene="holC"
                     /locus_tag="OO150_001701"
     CDS             complement(2747..3190)
                     /gene="holC"
                     /locus_tag="OO150_001701"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5515883.1"
                     /translation="MKNATFYLLDNDTTVDGLSTVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3350..4861)
                     /gene="pepA"
                     /locus_tag="OO150_001702"
     CDS             complement(3350..4861)
                     /gene="pepA"
                     /locus_tag="OO150_001702"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5515884.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5128..6228
                     /gene="lptF"
                     /locus_tag="OO150_001703"
     CDS             5128..6228
                     /gene="lptF"
                     /locus_tag="OO150_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5515885.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6228..7310
                     /gene="lptG"
                     /locus_tag="OO150_001704"
     CDS             6228..7310
                     /gene="lptG"
                     /locus_tag="OO150_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5515886.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7471..8973)
                     /locus_tag="OO150_001705"
     CDS             complement(7471..8973)
                     /locus_tag="OO150_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5515887.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(9103..10122)
                     /gene="ahr"
                     /locus_tag="OO150_001706"
     CDS             complement(9103..10122)
                     /gene="ahr"
                     /locus_tag="OO150_001706"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5515888.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            10318..10402
                     /locus_tag="OO150_001707"
     tRNA            10318..10402
                     /locus_tag="OO150_001707"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10352..10354,aa:Leu,seq:caa)
     gene            10566..11831
                     /locus_tag="OO150_001708"
     CDS             10566..11831
                     /locus_tag="OO150_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5515889.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLQANKGKTITPYDFAKINKSR"
     gene            complement(11886..12896)
                     /locus_tag="OO150_001709"
     CDS             complement(11886..12896)
                     /locus_tag="OO150_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515890.1"
                     /translation="MIYITSESKETLMIKYEGKMKKNIRVIPKNIHSKLRRLGNTVVA
                     GTSIAFTENQLKSGALEHLGIYFDNGVNAYETSVIPDPLQGKYSLKNVFGEEIVRKDL
                     PKETHYTEIESPNWGDSSNGTHTVRLPYEKYPRDIIPPTLIAIEINHKQPSDSHFIFN
                     FRVTRILDKQSNKFDDELLFDLNLLQENLGKCGIEDADKPISTYADTLIVSWEIFPPG
                     SKEETLARIFRGKNITSDKKNVAENRYDFFMSLEPKKIVTGNSTFSNYIGAMLEDDLV
                     VFENIEYGNAIYILYDNWDDISKLSRIDLLSGRAGSNFDRIIHSGNWKDEVRKKVAAG
                     RL"
     gene            complement(12847..13383)
                     /locus_tag="OO150_001710"
     CDS             complement(12847..13383)
                     /locus_tag="OO150_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515891.1"
                     /translation="MLKAIAGIAKYTWIYMAIFIISLMIISSSDINYVYNDFANYCTL
                     LVSVSGMVFTIMGIWLAFLYPNALQRIVNPKTIETVDFTEQLEDTRRLEAIVGSVLKS
                     SFVMITLLVVYLVKLVFFKSHLYLESQDVIKNTSLALIIAISYMQIEAVISVILANVM
                     FINDLHNKREQRDIDDQV"
     gene            complement(13384..14463)
                     /locus_tag="OO150_001711"
     CDS             complement(13384..14463)
                     /locus_tag="OO150_001711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515892.1"
                     /translation="MNVGHLNFFKVNKCGLYKVNDDNTYGLELSETFDLIQDWVGTKS
                     LALTIPWDPKEKPNSSKCYCKDIYKDENTGDFLVMLWKSDTDSTGSLLGASEDGEIGS
                     SSVVKYTNSYRGKKVIWGRPCFYWVIPELETIVSIKFDHSVCDSELFQDYVHSSITNR
                     VKHSKRVKNKTEKGYIRLSNTDDEDLYKYMYRFDMKLRSLETTHTELGKLIPKITHIV
                     RRETIIINPNDARADWLKTFSTLVPFVSGKKNAKTRQIEIKAEAKPSLNEVQEIIEKY
                     SAEDREKRLWDNVGFATDKGITWVDKYRMRDILNVPSDDYSTYSAAYIYEQISHKRKE
                     FISPILKELKALQSQPRIKKASGED"
     gene            complement(14861..15433)
                     /locus_tag="OO150_001712"
     CDS             complement(14861..15433)
                     /locus_tag="OO150_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HCR5515893.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAT
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(15507..16007)
                     /locus_tag="OO150_001713"
     CDS             complement(15507..16007)
                     /locus_tag="OO150_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5515894.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(16004..16738)
                     /locus_tag="OO150_001714"
     CDS             complement(16004..16738)
                     /locus_tag="OO150_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HCR5515895.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            17290..17556
                     /locus_tag="OO150_001715"
     CDS             17290..17556
                     /locus_tag="OO150_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5515896.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            17553..18152
                     /locus_tag="OO150_001716"
     CDS             17553..18152
                     /locus_tag="OO150_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5515897.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            18145..18432
                     /locus_tag="OO150_001717"
     CDS             18145..18432
                     /locus_tag="OO150_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HCR5515898.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            18425..18880
                     /locus_tag="OO150_001718"
     CDS             18425..18880
                     /locus_tag="OO150_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515899.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYQDIYPQDTVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            19016..19336
                     /locus_tag="OO150_001719"
     CDS             19016..19336
                     /locus_tag="OO150_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HCR5515900.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            19351..21684
                     /locus_tag="OO150_001720"
     CDS             19351..21684
                     /locus_tag="OO150_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5515901.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDVAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEVTRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPLDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            21850..22044
                     /locus_tag="OO150_001721"
     CDS             21850..22044
                     /locus_tag="OO150_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5515902.1"
                     /translation="MWIKDALVWIRATDPNLLIKNYLTFRFIDGLKIDWSEKQWGHKR
                     GHISLLCFIICFLSLTYFDI"
     gene            22262..23530
                     /locus_tag="OO150_001722"
     CDS             22262..23530
                     /locus_tag="OO150_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5515903.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     AKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANREKGITPFDYAKINRGNGE"
     gene            23921..>24216
                     /locus_tag="OO150_001723"
     CDS             23921..>24216
                     /locus_tag="OO150_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5515904.1"
                     /translation="MNFKKKLEEHFKQFEASPVLFVGSGVSRRYLGVPCWQDLLKHFA
                     EAIGENHIKLKTKSNGDLPEYAQLLVSAYAEKWWDTEEGQLALSEKEQEKTFIN"
BASE COUNT         5737 a         6288 c         6034 g         6157 t
ORIGIN      
        1 acaaagcctt cattcgccag cttgttctcg atacggctga tttcgccttc gatcttcgcc
       61 acttctttcg ccagacgcgc cagctcatct tctttgttga tgaggccagc catcgggatc
      121 agcagctctg cgccgtcgat gatcttcgta acggaaaccg gacctttgtc atcggcaggc
      181 agcacggtga tgctttccag acgcgccagg gtttgcagga agccacggtt ttcatttacg
      241 cgacgttctg catccgcgct gcaaccacgc agcagcagct ccagcggttt gcccggcgcg
      301 atgttcattt ctgcacggat gttacgtacc gcaacgatcg cctgtttcag ccactcggtg
      361 tcagccagtg cggcttcatc aacctgagat gcatcgtact gcgggaacgg ctgcagcatg
      421 atggtgtcgg cagtgatacc gcaaagtact ttcacacgct gccagatggt ttcggtgatg
      481 aacggaatga tcggatgcgc gaggcgcagc agaccttcca gtacagtcac cagcgtatgg
      541 cgagtaccgc gcagttcggc ttcggttcca ccgttcatta ccggcttagt cagctccaga
      601 taccagtcgc agaactggtt ccaggtgaac tcatacagaa tgcctgcagc gatatcgaag
      661 cggaagctgt ccagcgcttc gcggtacgct ttgatggtct ggttgaactc tgccagaatc
      721 cagcggtccg ccagcgacag cgtcatttca ccaccgttga agccgcaatc ctgaccttcc
      781 gtgttcatca gcacaaagcg gctggcgttc cacagcttgt tacagaagtt acggtagcct
      841 tccaggcgtt tcatatccca gttgatgtca cgaccggtag aagccagcgc cgccagggtg
      901 aagcgcaggg catcggtgcc gtgcggctca atgccgttcg ggaactgttt ctcggtgcgc
      961 ttacggattt tgtccgccag ctgcggctgc atcatattgc cggtacgttt ctccagcagt
     1021 tccggcagcg aaataccgtc aaccatatcc agcgggtcaa taacgttacc cttggatttg
     1081 gacatcttct ggccttcgtc gtcacgaatc aggccggtca tgtaaacggt gtggaacggc
     1141 acctgcggtt tgccattttc atctttgatg aagtgcatgg tcatcatgat catgcgggca
     1201 atccagaaga aaatgatgtc gaaaccagat accatcacgc tggttgggtg gaactggcgc
     1261 agtgcgtcgg tgttttccgg ccagcctagg gtagagaagg tccacagcgc agaagagaac
     1321 caggtgtcga gaacgtcttc gtcctgacgc agggcaacgt cggcaccgag gttattttcc
     1381 ttgcgcactt cttcttcgtt gcggccaaca taaacgttac ctgcttcgtc gtaccatgca
     1441 gggatacggt gaccccacca cagctgacga gagatacacc agtcctgaat atcgcgcatc
     1501 caggagaagt acatgttttc gtactgcttc ggtacgaact gaatgtcgcc gttctcaacc
     1561 gcctcaaccg ccggtttcgc cagtacatcg gcacgcacgt accactggtc ggtgagcatc
     1621 ggttcgataa ctacgccgcc acggtcgccg taaggcacgg tcaggtcgtg cggtttaatt
     1681 tcttccagca ggccgagcgc gtcaaccgca gcaacgactg ctttacgtgc agcaaaacgc
     1741 tccagtttct ggaactctgc cgggatttcg ctggaataaa cgtcagattc gttacctttg
     1801 gtatcgaaca cctgggcgct ttcacggata tcgccgtcaa aggtcaggat gttgatcatc
     1861 ggcagcgcgt gacgtttacc cacttcatag tcgttaaagt cgtgcgccgg agtgattttc
     1921 acgcagccgg tgcctttttc catgtcggcg tgttcgtcgc caacgatcgg aatacgacgg
     1981 ttaaccagcg gtagaatgac atatttgcca atcaggtctt tgtaacgcgg atcttccggg
     2041 ttaacggcca cgccggtatc gcccaacagg gtttccggac gggtagtcgc aaccaccaga
     2101 taatctttac cgtctgcggt tttcgcgccg tcagccagcg gatagcggat gtgccacatc
     2161 gaacctttcg attcgcggtt ttccacttcc aggtcagaga tagcggtgcg cagtttcgga
     2221 tcccagttta ccaggcgttt gccacggtaa atcaggtctt ctttatacag actaacgaaa
     2281 acttctttca ccgcattgga caggccttcg tccatcgtga agcgttcacg ctcccagtcg
     2341 acggagttgc cgagacggcg catctgacgg gtaatcgtgc cgccggattc cgctttccat
     2401 tcccaaattt tgtcgatgaa agcttcgcgg ccgtagtcgt gacgggtttt accttcttct
     2461 gcggcaatct tgcgctcaac gaccatctgg gtagcgatcc cggcgtggtc agtaccgacc
     2521 tgccacaggg tgtttttgcc ctgcatgcgc tgatagcgga tcatggtatc catgatggtt
     2581 tgctggaagg cgtgacccat atgcaaactg ccggtgacgt tcggcggcgg gatcatgatg
     2641 cagaaacttt cctggctttc atcgccatta ggcttaaagt agccctgctt ttcccagtgc
     2701 tcgtaaagcg gctgttcgat atcttgtggg ttatatgtct tttccattat ttccaggttg
     2761 ccgtattcag gttgaaacca gccacgcggt aggctttata gcgttcgcgc gccagttgtt
     2821 tcagagaatc ttcataagga acgaagtcta ccacttctgt gaaagcggtg gcaaaatctg
     2881 caaagcttgt tcgcagacta atcaatatat cgcgcgggct gctgctgcgt ttttgcggcc
     2941 aggcgatctc caccggcgca ccgccgcgcg gtccttctcc cgctaaatta tgcggaacaa
     3001 agctttctgc cggacgcgcc cacagggctt catccagccg gtaagcctgc ttttcatctt
     3061 cacaggcgat gagcacgcgc ttaccgctgc gccaacgttc tgcggcaatt tcacacacca
     3121 gttgctcaac ggtgcttaag ccatcgacgg tggtgtcatt gtccagaagg tagaacgtcg
     3181 cgtttttcat atatggggct tcttgtggtg gatttaaata cgcatcaggt atacaccgca
     3241 gcgcctgatg cgacgctgtt gtgtcttatc aggcctacga gttcagtgct gtgtaggtcg
     3301 gataaggcgt tcacgccgca tccggcaata acagccttgc ctgacgcaat tactcttcgc
     3361 cgttaaaccc agcgcggttt aacaggaact gtgccagcaa cgccaccgga cgaccggtgg
     3421 cgccttttgc tttaccagaa cgccaggcgg ttccggcgat atccaggtgc gcccagttgt
     3481 acttacgggt aaagcgcgac aggaagcaac ctgcggtaat cgccccacca ggacgaccgc
     3541 caatgttcgc catatcggca aaattggact ccagctgttc ctgatactcg tcacccagcg
     3601 gtaagcgcca tgcgcggtca ccggattgtt cagacgcggc aatcagttca tgggccagcg
     3661 gattatggtt cgccatcaga ccggtaatgt gatgacccag cgcgatcacg caggcaccgg
     3721 tcagcgtcgc cacgtcaatc accgcttccg gctcaaaacg ctcaacgtaa gttaacacgt
     3781 cgcacagtac caggcggcct tcagcgtcgg tattcagcac ttcaacagtt tgaccggaca
     3841 tggtggttaa cacatcgccc ggacgatagg ctcgtccgcc aggcatgttt tcgcagcctg
     3901 ccaacacgcc gataacgtta atcggcagtt gtagctccgc gaccatccgc atcacgccgt
     3961 aaaccgctgc cgcaccgcac atatcgtact tcatctcatc catgccttct gaaggcttga
     4021 tcgagatacc gccggagtcg aaggttaaac ctttacccac cagcacgatt gggcgtgcat
     4081 cttccgacgc gttgcctttg tactcaatca ccgacatcag cgattcgttt tgcgaaccct
     4141 gaccgaccgc cagataggaa tgcatcccca gctctttcat ctgctgttcg ccgataacgc
     4201 gggtgatgac attcttgctg tagctgtcag ccagctggcg cgcttgtgaa gcgaggtaag
     4261 cggcgttaca gatattcggc ggcatattgc cgagatcttt tgctgcttta atcccggcgg
     4321 caatcgccag accgtgcagg atcgcgcgct caccgctggt cagttcacgg cgggtcggca
     4381 cgttgaacac cattttacgc agcggacgac gcggttcgct cttgttcgtt ttcagctgat
     4441 cgaaactgta gagcgtctct tttgccgtct cgacagcctg acgcactttc cagtagttgt
     4501 tacggccttt aacgtgcagt tcagtcagaa agcagaccgc ttccattgag ccagtatcat
     4561 tcagcgtatt aatggttttc tgaataacct gcttgtactg acgctcatcc agctcacgtt
     4621 ctttgccgca accaataagg agaattcgct cggaaagtac attcggaaca tggtgcagca
     4681 acaatgtctg ccccggtttt ccttccagtt cgccccgacg tagcagggcg ctgatgtacc
     4741 catcgctgat tttatcgagc tgttctgcaa tcggagaaag gcgacgtggt tcgaagacgc
     4801 ccacgacgat gcaggcactc cgctgtttct ccgggctacc gctttttaca ctaaactcca
     4861 tgcactacgc tcctgaatct taaagacaac ggcggtggct acagatagaa ttgcaagctt
     4921 tcgtaactca tgtccgctgt tgcgatgact tcgtgttaat cttaacgtta ttacggcatt
     4981 ggcacgtcag aacaaaatct gagaggtgaa tccgttgagt ataattatct tagcgacgat
     5041 ttcgacgact caagagaata aatgacgttt aagccatgaa acaagctaaa atcctgcaaa
     5101 agacgagttt ttacgggcgt atttaaagtg ataatcataa gatatctggt gcgggagacg
     5161 ctcaaaagcc agctggcgat actcttcatc ttgcttttga tcttcttctg tcaaaagtta
     5221 gtgaggatcc tcggcgcagc ggttgacggc gatattccgg cgaatctggt gctctccctt
     5281 ctcgggttgg gcgtgccgga aatggcgcag cttatcctgc cattaagcct gttcctcggg
     5341 ctgctgatga cgctgggcaa actgtatacc gaaagtgaaa ttacggtaat gcatgcctgc
     5401 ggcctgagca aagcggttct ggtgaaagcg gcaatgatcc ttgcggtatt cactgcaatc
     5461 gtcgcggcgg ttaacgtgat gtgggcggga ccgtggtcat cgcgtcatca ggatgaagtg
     5521 ttagcagaag cgaaagcgaa ccctggcatg gcggcgctgg cgcaagggca attccagcaa
     5581 gcgactaatg gcagctcggt gctgttcatc gaaagcgttg acggcagcga tttcaaagat
     5641 gtgttcctcg cgcaaattcg accaaaaggt aatgcacgtc cttctgtggt ggtggccgat
     5701 tccggacatt taacccagct gcgcgacggc tcccaggtcg tcactctcaa ccagggaacg
     5761 cgcttcgaag gcactgcact gttacgtgat ttccgcatta cggacttcca ggattatcag
     5821 gcgatcattg gtcaccaggc ggtagcgctc gacccgaacg ataccgacca gatggacatg
     5881 cgtacattgt ggaacactga caccgatcgt gcccgcgcag aactgaactg gcgtatcacg
     5941 ttggtattca ccgtgtttat gatggcactt atggtcgtac cgctgagcgt ggttaacccg
     6001 cgtcagggac gcgtactatc gatgctgcca gccatgctgc tgtatctact tttcttcctg
     6061 atccagacct ccctgaaatc gaacggcggt aaaggtaagc tggacccgac gctgtggatg
     6121 tggaccgtta acctgattta tctggctttg gcgattgttc tcaacctttg ggacaccgtg
     6181 ccggtccgcc gcctgcgcgc cagtttttcg cgtaaaggag cggtgtgatg caaccttttg
     6241 gcgtacttga ccgctatatc ggtaaaacta ttttcaccac catcatgatg acgctgttca
     6301 tgctggtgtc gctgtcgggc attatcaaat ttgtcgatca gctgaaaaaa gccgggcagg
     6361 ggagttacga cgcgttaggc gcaggaatgt ataccttgct gagcgtgccg aaagatgtgc
     6421 aaacattctt cccgatggcg gctctgcttg gggcgttgct tggtcttggg atgctggcgc
     6481 agcgcagcga actggtggtg atgcaggctt ctggtttcac ccgtatgcag gttgcgctgt
     6541 cggtgatgaa aaccgccatt ccgctggtct tgctgacgat ggcgattggt gaatgggtcg
     6601 cgccgcaggg cgagcagatg gcgcgtaact accgtgcgca ggcgatgtac ggcggctcgt
     6661 tgctctctac ccagcaaggc ttatgggcga aagatggcaa caacttcgtt tacattgagc
     6721 gggttaaagg tgacgaagag ttaggtggca tcagtattta tgcctttaac gagaatcgtc
     6781 gtctgcaatc cgtacgctat gccgctactg cgaagtttga cccggaacat aaagtctggc
     6841 gtctgtcgca ggttgatgaa tctgatctga ccaatccgaa acagatcacc ggttcgcaga
     6901 cggtgagcgg cacctggaaa accaacctca cgccggacaa actgggcgtg gtggcgctgg
     6961 acccggatgc actctcaatc agcggtttgc acaactacgt gaagtatctg aagtcgagcg
     7021 gtcaggatgc cggacgttat cagctcaata tgtggagcaa aatcttccag ccgctatccg
     7081 tggcggtgat gatgctgatg gcgctgtcgt tcatctttgg cccactgcgt agcgtaccga
     7141 tgggcgtgcg tgtggtcacc ggtatcagct tcggttttgt cttctacgta ctggaccaga
     7201 tcttcggccc gctgacgttg gtttatggca tcccgccgat catcggcgca ctgttgccaa
     7261 gcgccagctt cttcttaatc agcctgtggc tgttaatgag aaaatcgtaa ccgaatgaac
     7321 gaataaaggg agcgaaagct ccctttatta ttgttagcaa agtgtgcttc gttcatgcct
     7381 gaaaaatcat gaaaatccaa tatattgcag tggtgtaggc atgataagac gcgccagcgt
     7441 cgcatcaggc aaagacaaat ctcgcctgac ttaccttctt cttcccccca gcaaactccc
     7501 caacatccca cgtacaatct gattcgtcac ctggcgggcg gcgcttttcg ccattgtttg
     7561 caccacacca tctttcttcc cgccgcgtgg tccggtagtg ccaaacaaaa tatccttcaa
     7621 tccaccaaga ataccgtcat ccaccgccac cgctttccct ttcgcgggag gattattttg
     7681 ctgcttggta ctggcctgaa agcctttttg caacatctca taggcggatt cccggtccac
     7741 gtcatcctcg tacttgccat acaccgggga gtggttaatc aagccattgc gctcatcttc
     7801 cgtcaccggt cccatccgcg aacaaggcgc aatcaccatc gcccgttcca ccacagaagg
     7861 acttcctttt gcatcgagaa acgagattaa cgcctcgccg gtccccagtt cctggattgc
     7921 cttttcggta tcaaacgccg gattggcccg catggtttgc gccgcagctt ttaccgcttt
     7981 ctgatctttg ggcgtaaaag cccgcaaagc gtgttgaacg cgattaccga gctgcccgag
     8041 cacattatcc ggaatatcag acgggttttg cgaaacgaac cagacgccca cgccttttga
     8101 gcgaataagc cttattacct gctcaatctt atccagcagt acctgcggtg catcgttaaa
     8161 cagcagatgt gcctcgtcga agaaaaacac cagttttggt ttctccagat cgcccgcttc
     8221 cggcaattgt tcatacaact ctgaaagcat ccacagcagg ctggcggcgt acagtttcgg
     8281 catctgataa agcttctcgg cgctgaggat attgataacg cctttaccgt tggtatcggt
     8341 gcgcatccag tctttgatat ccagcatcgg ctcgccaaag aaatgcgccg caccttgctt
     8401 ttccagcgac agtaaaccgc gctggatggc accaaccgat gcgctactga tattaccgta
     8461 ctgattctgg aaagatttgg cgttatcgcc gatgtactgg gtaatcgccc gcagatcttt
     8521 aaagtcgagc agcaacaatc cctgatcgtc agcaatacgg aagatgatat tcagcacgcc
     8581 agattgcaca tcgttgagat tcaacagccg cgccagcaac agcggcccca ggtctgaaac
     8641 cgtcgcccgc accggatggc ctttctcgcc aaagatatcc cacaccacca ccggattagc
     8701 atgcggttgc cagtcattga cgccgatatt tttaagcctt gtgagcagtt tttccgacgc
     8761 cgttcctgcc tgcgcgatac cggtcagatc gcctttcaca tcagccataa acaccggcac
     8821 gccgatttcc gacaatgact ctgccagttt ttgcagcgta acggttttac ccgtccccgt
     8881 tgcgccagta atcagcccgt gacggttagc cattcccggc agtaaaaaaa gttctgtgtc
     8941 cggcgtgcgg gcaattaata ggggttcgct catgagattt cctccagttt cctgcctgga
     9001 gtataggcaa cggctgcgca ctcttcaccg ctaaattcag cagattgcga gacgccttcc
     9061 aaagagattt aagtgccggg cgacaggcac ccggcaaagg gatcagaagt cggctttcag
     9121 aaccacgcgg taacgcgcct taccgtcgcg cacatgcttg atggcatcgt taattttcga
     9181 catcgggaac agttcggtgg tcggcgcaac cttgctacgg gcggcaaagc gcatcagctt
     9241 tcgcagctca taaggcgtgc cggtagcaga accagagatg ctgcggtcgc ccgcaattaa
     9301 tgtaaaggcc ggaacagaca gcggcgtgag aaccgcaccg accgtatgga aattaccgcc
     9361 ataggtcagc gcctcaaaat aaggctgcca gtcgaggctg acgttcacag tattgataat
     9421 gagatcaaac tgccccgaca gggctttcag tgcctgcgga tcgcggctat tcaccacttt
     9481 atcggcaccc atcgccagta cttcctgctc tttcgccgga ttagaactaa aggccgtcac
     9541 ctcgcatccc attgcgtgca gaagttttat agcgatatgc cccagcccgc caataccaat
     9601 taccccaacg cggctggtag cagtgatatg gtgcatcaac agtggtttaa agaccgtgat
     9661 accgccgcac aacagcggcc cggcggactc gatatcaata ttttcaggca gtggaatcac
     9721 ccattgccag tccgcacgca acttctcggc aaagccaccg cgattcataa tcgtcggcac
     9781 cgcaccttgc tcgcagttga tctgattacc gctaatacag gcgtcgcagt gaccacagct
     9841 acgcgccgtc cagccaatcc cgacacgctg accgacctgc aaacctttat cctgcgcggc
     9901 gctcccgagt gccaccacgc gcccaatcac ctcatgcccg gcaaccagcg gatattgtga
     9961 aaatccccat tcattgtcga tcatcgatag atcggaatgg cagatcccgc aataatccac
    10021 ctgcacttca acatcttgtg gcctcagctc accgggatcg tactcataaa cttccagttc
    10081 gccgcccgct tcttttgcgg catagctttt tatcatcgac attttttggt ccttctctgg
    10141 tgttgtttgg gaagtgtaga gcatggcagg gcggggtgcc tggagtgtga caaaggttac
    10201 acatcgctgt gtgcaatgct gaaaatttca gcacttagcg aggtgcgagc aagctggcgc
    10261 ttgcatggtg gcgtgcgaca ggtataatcc acaacgtttt ccgcatacct cttcagtgcc
    10321 gaagtggcga aatcggtaga cgcagttgat tcaaaatcaa ccgtagaaat acgtgccggt
    10381 tcgagtccgg ccttcggcac cattcttact tccaagacca tccgagaaag tccaaatatc
    10441 cctttaaaat caatgcttgc agcgattttt tcgtcctgcg tcttccgagg ttgtccgttg
    10501 aaatccggat atcattgggg gcataattgg gggcatctta acttcgatta gaaatgtgcc
    10561 cccaaatgaa gctcaacgcc agacaggtcg agaccgcaaa gccaaaagac aaaacctaca
    10621 aaatggccga tggtggtggt ttgtatcttg aggtttcggc caaaggttcc aaatactggc
    10681 gcatgaaata cagacgtccc tctgacaaaa aagaggatcg tctcgcgttt ggtgtttggc
    10741 ctactgtgac gcttgctcag gcaagagcaa agcgcgatca agctaaaaag ctgttagcgc
    10801 agggcattga tccaaaagcc gaacagaaag aagctcaggc cgagaattcg ggggcatatt
    10861 ctttcgaaac tatcgctcgt gaatggcatg ctagtaataa gcgctggagt gaagatcacc
    10921 gatcacgcgt tctacgttat cttgagcttt atatctttcc tcatattggt tcatctgaca
    10981 tccgtcagct caaaactagc cacctattag ccccgattaa aaaagttgat gccagtggta
    11041 agcatgacgt tgcacagcgc cttcagcagc gtgtcacggc tattatgcgt tatgcagtac
    11101 agaacgatta catcgactca aatccagcca gtgatatggc cggtgcgcta tcgacaacca
    11161 aagcgcgaca ttaccccgct ttgccctcca gccggttccc tgagtttctt gctcgtcttg
    11221 ctgcatatcg tggccgtgta atgacgcgga tcgcggttga gctttcctta ctaacttttg
    11281 tgcgttccag tgagttacgt ttcgcgcgtt gggatgagtt cgacttcgat aaatctctct
    11341 ggcgagtacc tgcaaaacga gaagaaatta aaggtgtgcg ttactcgtac cgtggcatga
    11401 agatgaaaga ggagcatatc gttcagctta gtcggcaggc gatgattttg ctaaaccagt
    11461 taaagcagat tagtggagat aaagagctgc tttttccggg tgatcatgac gcaactaagg
    11521 ttatgagcga aaacacggta aatagcgcat tgcgtgcgat gggctatgac acgaaaaccg
    11581 aagtttgcgg gcacgggttt agaacgatgg cccgtggtgc gttgggcgag tccggattat
    11641 ggagtgaaga tgctatagaa cgacagttga gccattcaga gcgtaataat gtacgtgctg
    11701 cttatattca cacctctgaa catttggatg aacgtcgatt gatggttcaa tggtgggctg
    11761 attatctcca agcaaataag ggcaaaacaa taacacctta tgattttgct aaaataaaca
    11821 aaagtcgata gtgaaaataa acgcctataa tatataggcg tttatttctg tattaaacac
    11881 tttgatcata atcttccagc agcaactttt tttcgtactt catctttcca attgccacta
    11941 tggattattc tatcgaaatt acttcctgct cttcctgata ataagtcaat tcgagatagc
    12001 ttgctgatat catcccagtt atcatagagg atatatatag cattaccata ttctatgttt
    12061 tcaaatacaa ccaaatcgtc ctccagcata gctccaatat aattactgaa cgtgctgttt
    12121 cctgtgacga tttttttagg ttcaagactc atgaagaagt catatctatt ttcagcgaca
    12181 tttttcttgt cggaagtgat atttttacct ctaaaaattc ttgctaaagt ttcttcttta
    12241 ctacctggtg gaaatatttc ccatgagact ataagtgtat cagcataagt cgatatgggt
    12301 ttatcagcat cctcaattcc acatttacca agattttctt gaagaagatt taaatcaaat
    12361 aataattcat catcaaactt atttgattgt ttgtctaaaa ttctagttac tctgaagtta
    12421 aatatgaaat ggctgtccga gggctgtttg tgattaattt caatggctat gagtgttggt
    12481 ggtataatgt ctctgggata tttctcgtaa ggtaatctaa cagtatgtgt accattagag
    12541 ctatcacccc agttaggtga ctcaatttca gtgtaatgag tttcttttgg gaggtctttt
    12601 ctgacaattt cttcaccaaa aacattttta agtgaatatt taccctgcaa tggatctggt
    12661 atgaccgacg tctcataagc attaacacca ttatcaaaat atattccaag gtgctctaat
    12721 gctccacttt ttaattggtt ttccgtaaat gcaatagaag tgccagcaac aactgtatta
    12781 ccaagccttc ttaacttgct atgaatattt ttaggtataa cacgtatatt ctttttcatt
    12841 tttccctcat acttgatcat caatgtctct ttgctctcgc ttgttatgta aatcattgat
    12901 aaacataaca tttgcaagta ttacgctaat tactgcctca atctgcatgt acgaaatagc
    12961 aataattaat gctaaagatg tgttttttat tacatcttgg ctctctaaat aaagatgact
    13021 tttgaaaaac actagtttaa ctaagtatac aacaagtaaa gtaatcataa cgaaagatga
    13081 ttttagaaca cttccaacta ttgcttctaa tcttcttgta tcttccagtt gttcggtgaa
    13141 atcaacggtt tctatagttt tggggttcac aatacgctgc aaagcatttg ggtaaagaaa
    13201 cgctaaccaa attcccataa tagtaaaaac cattccagaa actgatacga gaagagtgca
    13261 atagttggca aaatcgttat acacatagtt aatatcggat gacgaaataa tcatcagcga
    13321 tataatgaat atcgccatgt atatccatgt gtatttggct atgcctgcta tagctttcaa
    13381 tattcaatcc tctccagatg ctttctttat tcttggttga gattgtaatg cttttaattc
    13441 ttttaatata ggggaaataa attcttttct tttgtgtgat atttgttcat aaatatatgc
    13501 ggcggaatat gttgaatagt cgtcagaagg gacattgaga atatcacgca ttctatattt
    13561 atccacccaa gttataccct tgtctgtagc aaatccaaca ttgtcccaaa gacgtttttc
    13621 tctatcctca gccgaatact tttcaatgat ttcttgaact tcattcaagg atggttttgc
    13681 ttctgctttt atttcaattt gtcttgtttt ggcatttttc tttccggaca caaatggaac
    13741 gagagtgcta aaggtcttta accaatcagc tcgtgcatca tttggattta taataatagt
    13801 ttccctgcga actatgtgtg ttatttttgg tattagttta cctaactcag tatgagtggt
    13861 ttctaagctt ctgagcttca tatcaaacct gtacatatat ttatataagt cttcgtcatc
    13921 ggtattagat agccttatat aacctttctc ggttttattt ttaacccttt tgctatgttt
    13981 aactctatta gtaatagatg agtgtacata gtcttgaaat agttcagaat cacaaacaga
    14041 atggtcgaat ttaatggata ctatagtttc aagttcaggt ataacccaat aaaagcatgg
    14101 ccgcccccag ataacttttt tccctctgta gctattagta tatttaacca ccgatgagct
    14161 tccgatttca ccgtcttcac tagctcctaa taaagagccc gtactatcag tatcagattt
    14221 ccataacatg actaagaaat caccagtatt ttcatcctta taaatatcct tgcaataaca
    14281 ctttgaactg tttggttttt cttttggatc ccatggtatc gtgagtgcaa gagatttagt
    14341 tcccacccaa tcctgtatga ggtcaaatgt ttcgctaagt tctaaaccat aggtattgtc
    14401 atcatttacc ttgtataaac cgcacttatt aaccttgaag aaattgagat gccctacatt
    14461 catgctcttt ccttagcttc ttatcttggg atatgtatca tgatcaaaaa ttaagcataa
    14521 ccccattgac ttcgtgcggg caatgttttc accctagaat ttgatcggtt taacgttaaa
    14581 tattcataag ttacatgagc atttttcact tcttcccaat tacagacgtc atgagctgaa
    14641 tattaataaa ataattatcc tcacggcatg gcgcgcaatg ctctccccgt cacgcccgcc
    14701 cgctttgcag ggcggtttta atgcagttgc acagtcacgc tcaggctgcg ccgggaatgg
    14761 cgcggtctgc agaaaatgag gcaggaaaac gcatgcaaag ccatgcacct tatcgatgca
    14821 tggctttttt cagtaaaaac gggcggattt tcggggaatt ttacacagac tgacgtgatg
    14881 ccagttgcgc acttttacgc gaaaaaatca tgttctgcgc aggggtgaat ttttcacggc
    14941 tgtcatccac cgaagtcgcg tcaggcctga atccgatggt cgttaaaatg tcactatcct
    15001 gtgcggaata attaattttt tcccccttcg caagccagga cagaagggct tcacgcaggg
    15061 catctgtggc acgctgtatg gcacagtttc gggcaatggc cgtcagctca ctgtagccca
    15121 tcagctccgg tgccagtgcc gccgccagtg ctgtgccgtg ctgctgcata aaatcattca
    15181 gccggtcgcg gatgctgatg tgctgaacgg cttcatgcga acgaatataa cgaccggcag
    15241 cctgattcac ctgccatttt ctgacttcga taatattgcg taattcgtcc aggcgactga
    15301 cgtttctgcc ttccccggac agaagccgca gatattcctg ttcggccgca gccagctcat
    15361 ttttgcgttg cagccatgct gctttgttat tctgacaggt gtcaaaggcc tgctgtaagg
    15421 ctgtgctttc catcgttatc tctttctcat catgctgaag aataaaaata cggtgtgcgg
    15481 cgacggccgg tgttaaccgg cagccctcat tccagacgca gcgaatatga ttgtgttttt
    15541 aagcgtactg gcggcagttc ctgtttttca tgcaggcttt ctgccagttc gtccggcgtg
    15601 accgggcgga caataaaacg gttgatggtc tgaagcgttt taaacaccag accacagccc
    15661 ggatccgtgc acacataaaa acgctcggtg acttcctgag acagacgccg cgatgttctt
    15721 gacagtgcaa ggcctttaca tctccgacaa caatatccgg taacaagcat tcttttcggg
    15781 cgtttcatac tgccggaggc tgacgtcagt gaatcgcggt atctctgttt gcctgaaatg
    15841 tattccattc ctgaatcttt acagtcagag aaaaagcttt cactcgcttc aaatgtcgca
    15901 gagcaataaa tattccggca ctgtgcaatc attatcttgg tgccatcgtc catgaaatgt
    15961 gcgcgacggg tgtgagcaac atgtccacac gacgggcagt aaatcatgac agcagtcctc
    16021 tggccttaag ctctgctccc tgctggtcta ttttgtcctg ccacaccttg cgctgtgccg
    16081 gtgtgcctgc cacctcataa tccatgtgcg ggagtgttgc cgctgacagt ccggtcagcc
    16141 ggagaaccgg ctcgccggtg aggctgattt gcatctgttt aattttctgt tccagcgatg
    16201 atttcacctg ctgcatgaca gccttttccg gtgcgacgta gccctgatgg ccggtggtgt
    16261 tggcgagcgg attttcctgt accaggatac tcagatgcat tgcccggaca agcgcctcac
    16321 aggtttcatt cagggcgtgt tccagctcat gctctgcata cagactcagc aggtgatgat
    16381 gtgccttccg gtaggcggtg gccgtgctgt cacacgcccc tttcaggcgt tcacgttcaa
    16441 aattcagcac cacggccaga ttgtcatatt cctgtaccag ctcccggcgt gccacgcgct
    16501 caatgtggcg ctgtttcagc tcgtcgctca ggacaccacc ggctgcacga aaggccgtgc
    16561 gccagtcgtc agcgtcattt ccgtcggcct gcgccagcgc atttttttcc tgctctgccc
    16621 gttcaatggc cgtgacggtc tcatccatca ggcgggcgtt ctcaaggtgg gcggctctgg
    16681 ccttttccag ttgtgccagt gcgggttgca gatattcagg gatggtgttg tcagacattt
    16741 tccggctcct cgtcacttca ggttaagaaa attgtgacgt acaccggaca acaacacgac
    16801 gcattgcaga tgtgccagcc ctgacacagg agactcatcc tcagaccggc aagccaggaa
    16861 aaggtcgcag gaaaaaccgg cttactgttt gtttttttat attttactgt tcacctctgt
    16921 tcaccataag aaaaaagata agtaatacag taagttaaag ggtgaacaat cgcagtaatg
    16981 actgttcacc gtctgttcac cactgttcac ccgctcatag actttttgtg ctgtttatta
    17041 ctttttattt ttattaatcc actgaaataa ataagaaaaa acaatttgta tttcactata
    17101 attttttcca gctccttcca gagcgtttta aagccatctg gcgctgatgt ataaaaaata
    17161 cacagccatt gtaagactgc cggaacaaat cccccctgtt gcatctgcca aaaatattca
    17221 caaaataaag cgctacccga agccggacgg acttatccgg tgctgtatgg acattaacga
    17281 ggtagcccga tgcaagctgt tttttcttcc ccgtctcccg cccctgtgac gccactgatg
    17341 ccgctgccgg acatcacgca ggagcgtttt ttacgtctgc cggaagtgat gcacctgtgc
    17401 ggcctgtcac gctcgaccat ctatgaactc atccgtaagg gggaatttcc gccgcaggtg
    17461 agtcttggcg gtaaaaatgt ggcctggctg cactctgaag tcaccgcatg gatggccgga
    17521 cgcattgccg gacgcaaacg ggggtacgac gcatgatgat gcccgctctg caaaaactcc
    17581 ctttttctgg cttgcctttt tccggcattt gcggatatag tttttccgct gccgcaaaat
    17641 cggcagccgg gcgtgagaac ccgagcaatt cacaggcgac atatgacgcg ccacgcgtct
    17701 ttttttgtgt cgcaatcaat gccacagagc gccagattat ggtgtggtgt gtggtcagtc
    17761 gtgcagatgg cattccgtgc atcctgcccg cgtccgctca ttatgccgca gagtcaatgg
    17821 tagctcaggc ggggcagcct ccgggctggc cggtatcctg tgaagccggt attctcaccc
    17881 ccgtctgggc tatcgccatc gagcgtgaga actccggcga tagcgttatt tgctattcac
    17941 aggaggctgc catcatggct acaaccctta ccccctcaca ccctgaattt gtctttgtgt
    18001 ttgcggctgt ccgtcgcgca gaccgtcatc cccgtatctg tatgcttcgc accgtggccg
    18061 gtgatgaacg cagtgcccgc cgttcccttg tccgtgacta tgtgctctcc cttgctgccc
    18121 gtctgccggt ggtggaggtg tcccgtgcgt aataaaaaag cccctcagac cgtctcagca
    18181 cgtcatgacg cccgtgaaca cctcagcatt gaggcttacc ataagctcaa ccgcgccagc
    18241 gccgtatccc agtttgttgg gggtgatttg attcaccgtg aactctccgg cctgcatcag
    18301 ctctacattc cgcatatttt cagctacctg aatgaagata ttgattttgt gctgaatgag
    18361 ctgaaagcca aaggcctgtg ccgcgatttt ctcgcccagc agaaagaccg gggagacagg
    18421 acgcatgttt gattttcccc agcccggtga gatttaccgt tctgccggtt ttcccgatgt
    18481 ggccgtggtc ggcattctgg aagacggtat tccgtgggaa atgccgtacc gctgcccgga
    18541 cattgtctgg aacccgtacc gccgtaaatt cagtatcctt gtgcgtatcc tcgccgacgg
    18601 gcgcaccaca gacatcccgc tggggcgttt tctgcgggaa tttacctgtg accgtcctga
    18661 cctgtttaaa cgcagccccg taaaccggca tgcggtactg aaagaaatgg ccggagaccc
    18721 ggaattacag aaatggcggg agaaatatca ggatatttac ccgcaggaca ctgttccggt
    18781 cagccgggca gcaccggtag cgcgggaatg gcgggaaatt ccccgcacgg agcctgaccc
    18841 ggaaatcacc ccggataaca gttaccgcaa ttatctgtaa ttaaaaaacg acacccgaaa
    18901 aattaaatgt gcgtattcgc gcagggatac gcacgtcttc aggagacgca gatatgcctt
    18961 atcagttaat gcaaccggca cggaatgcag tcatctgtca cagggaggaa agcaaatgaa
    19021 aacaccctta ccgcccgtct tacgcgcagc cctttaccgt cgtgctgtcg cctgtgcctg
    19081 gctgaccgtg tgcgaacgtc agcaccgcta cccgcatctc acccttgagt cactggaggc
    19141 ggccatcgcc gctgagctgg aaggctttta tctgcgccag cacggtgagg aaaaagggcg
    19201 tcagatagcc tgtgccctgc tggaagattt aatggaatcc ggccccctga aggccgcacc
    19261 gtcgctgtcc tttctcgggc tggttgtgat ggatgaactc tgtgcccgtc acataaaagc
    19321 gccggtactg cactgaagga gaacaacacc atgaaaatga acgtaaccgc caccgtcagc
    19381 catgcgctcg gccactggcc gcgtattctc ccggcgctgg ggattcaggt gctgaagaac
    19441 catcatcagc cctgtccggt ctgtggcggg agtgaccgct tccgttttga tgacagggag
    19501 gggcgcggca cctggtactg caatcagtgt ggtgccggtg acggcctgaa actggttgaa
    19561 aaggtgtttg gtgtctcccc gtccgacgtg gccgcaaagg tggctgccgt gaccggcagt
    19621 ctgcccccgg ctgacccggc agtgacggcc gccgccgttg ctgaaacaga cgctgcccgg
    19681 aagaacgccg ccgcactggc acaaaccctg atggcaaaaa cccgtcccgg aaccggtaac
    19741 gcctacctga cccgcaaggg ctttcccggc cgggaatgcc ggatgctgac cggcacacac
    19801 agagccggtg gcgtgagctg gcgcgccggt gaccttgtgg tgccactgta tgacgacagc
    19861 ggcgaactgg ttaaccttca gttaatcagt gctgacggcc gtaagcgcac cctgaaaggc
    19921 ggacaggtca ggggcacctg tcacaccctt gaaggacaga atcaggccgg aaaacgtctg
    19981 tggatagcgg agggatacgc gaccgcactt accgtacatc acctgaccgg tgaaacggtg
    20041 atggtggcgc tttcttccgt gaacctcctt tctctggcca gccttgcccg gcagaagcat
    20101 ccggcctgtc agattgtcct tgccgcagac cgtgacctca gcggtgacgg ccagaaaaaa
    20161 gccgccgcag ccgcagatgc gtgtgaaggt gttgttgccc tgccgccggt cttcggtgac
    20221 tggaatgatg ccttcacgca gtacggcggg gaggtcaccc gtaaggccat ttacgatgcc
    20281 atccggccac cggctgaaag cccgttcgac accatgagcg aagcagagtt ttccgccatg
    20341 agtaccagcg aaaaggccat gcgtatctat gagcattacg gcgaggcgct cgcggtcgat
    20401 gccaacggcc agcttctgtc ccgctatgaa aatggtgtct ggaaggtgct gccgccactg
    20461 gactttgccc gggatgtggc cgggctgttt cagcgtctgc gcgcgccgtt ctcctccggg
    20521 aaggtggcct ccgtggtgga caccctgaag ctgattattc cgcagcagga agccccctcc
    20581 cgccgcctga ttggctttcg taacggtgtg ctcgacacac agaacggcac gttccacccg
    20641 cacagtccgt cacactggat gcgtaccctg tgcgatgtgg atttcacccc accggtggaa
    20701 ggggaaacgc tggaaaccca cgcccccgcg ttctggcgct ggcttgaccg tgccgccggt
    20761 ggccgtgcgg aaaaacgcga cgtgattctg gccgcactgt ttatggtgct ggcaaaccgc
    20821 tacgactggc agctctttct ggaggtgacc ggtcccggcg gcagcggcaa aagtatcatg
    20881 gccgaaatcg ccaccctgct ggccggggag gataacgcca cgtcggccac cattgagacg
    20941 ctggaatccc cgcgtgaacg tgccgcgtta actggcttct cactgatacg cctgccggac
    21001 caggaaaaat ggagcggcga cggtgccgga ctcaaggcca tcaccggcgg cgatgcggtg
    21061 tccgtggacc cgaaataccg ggatgcgtac tccacgcata tcccggcggt gattctggcc
    21121 gtgaacaata acccgatgcg cttcaccgac cgcagcggcg gcgtgtcacg ccggcgggtg
    21181 attattcact tcccggaaca gatagccccg caggagcgcg acccgcagct taaggacaaa
    21241 atcacccgcg agctggcggt catcgtgcgt cacctgatgc agaagttcag cgacccgatg
    21301 ctcgcccggt cactgcttca gtcccagcag aactcagacg aggcgctgaa catcaaacgg
    21361 gatgccgacc cgacgtttga ttttatcggc tatctggaaa ccctgccgca gaccagcggc
    21421 atgtatatgg ggaacgccag tatcatcccg cgtaattacc gtaaatacct ctatcacgcc
    21481 tatctggcct acatggaggc aaacggctac cggaatgtac tcagtctgaa aatgttcggg
    21541 ctggggctgc cggtgatgct gaaggaatac ggactgaatt acgagaagcg ccataccaaa
    21601 caggggatac agaccaacct gacactgaaa gaggaaagct acggcgactg gctgccaaaa
    21661 tgtgacgacc ctgcaacaac ctgacccacc tgaccggcat ctgccggtct ttttttatcc
    21721 cgacctcccc cgaaggtgaa caatccactg ttcacccttc accgtatatt cacccgttat
    21781 cacactgaaa ttaaaagaga aaaatgaaag gtgaacagtg tgaacagttc tttcgaaaaa
    21841 aaatttttta tgtggataaa agatgcactg gtttggatcc gggctacgga tccaaactta
    21901 ctgattaaaa attatttaac tttccgattt attgatgggc tgaaaataga ttggtcagaa
    21961 aaacagtggg ggcacaaaag ggggcatatt tcattgttgt gttttattat ttgtttttta
    22021 tcattaactt attttgatat ttgagtccgg ccttcgcacc aaaagtatgt aaatagacct
    22081 caactgaggt ctttttttat gcctgaaatc cgcgctacac aaggcttttc cagcttttcc
    22141 cctcaactga agtcaacctg actcaaccca catcaacagg cttgtgagta tactaatgag
    22201 tatatttgcc gattcgatat tgcttgtata ctcacgagat accaatggaa ggaagaccat
    22261 tatggcccta acggatacta aagttcgctc tgcgaaacct gaggagaaag agtattcact
    22321 tgttgacggc gacggcatgt ccttacttgt aaaacctggc ggttcaaagt attggcgatt
    22381 tcgtttccgt tttggcggta aacaacattt gatggcgttc ggcgtttacc cggatgtttc
    22441 gctggcggat gcgcggaaga aaagggaaga ggccagaaag ctggtagctg ctggtatcga
    22501 tcctcgtgag cataagcgtg ctgtgaaaga agagcaggcg aaagagatta ttactttcga
    22561 gaaggttgcc agagagtggc tcgtaaccaa ccaaaaatgg tcggaagagc atgctaatcg
    22621 tgtaaaaaag agcctggagg acaatatctt cccggcaatt ggttcccaca atattgctga
    22681 actgggcacc cgcgatctgt taattcctat taaagccgtg gagaaatccg gacgtcttga
    22741 agtggcttcc cgccttcaac agcgcaccac ggccatcatg cgttatgcag tgcaaagcgg
    22801 gttaattgat tacaatccgg cgcaagagat ggcgggggcg gtagcttcca gtaatcgaca
    22861 gcatcgtccg gcgctggagt taaagcgtat cccggaatta ctccagaaaa tcgatgaata
    22921 caccggcagg ccactaaccc gctgggcaac cgaacttacc ctgcttattt ttattcgatc
    22981 cagtgagctg cgttttgctc gctggtcaga aatcgatttt gaaacgtcaa tgtggaccat
    23041 cccgcctgag cgagaaccta ttcccggtgt gaaacattct cagcgaggag caaagatgcg
    23101 tacacctcat ctggtgccgc tttcaaagca ggcgctagcg atcctgaagc agataaaaca
    23161 gttctgtggc gaacatgagc tgatttttat tggcgatcac gatccgagta agcccatgag
    23221 tgaaaacacg gtgaacagtg cattgcgggt gatgggctat gacaccaagg ttgaggtttg
    23281 cggccacggc ttccgcacta tggcctgtag ctcattgatt gagtcagggt tatggtcgaa
    23341 ggatgcggtg gaaaggcaga tgagccatat ggaacgcaat tcggtgaggg cggcgtatat
    23401 ccataaagcg gaacatctgg atgagcgcaa gctgatgttg cagtggtggg ctgattttct
    23461 ggatgcgaac cgggagaagg ggattacgcc gtttgactat gcgaagatca atcggggtaa
    23521 tggcgagtga gttatctaaa agcgcacatt cgcctctaaa ttgaaagctg ctgacttatt
    23581 caaacgagga attggtcagc agtgccaaat agccgagttt tcactaaggt aagccttttt
    23641 attgaaccta atcggcagta atgctccacc taagcctaaa tttaaagtca tgattactta
    23701 taaaagggga gtggaccctt gattgagccc tacatcgtaa caaaggtaca gcaattcggc
    23761 tacatcagga gtgatagtca attagttaat ctaataatta attgaatcta aataattaaa
    23821 tttcttacgt aaaattgacc aatttactgg tggtgtaagc cgcaatatct taatgtaatc
    23881 agtataaagg ggcttgattt cttttaaaga tagtatccgt atgaatttta agaaaaaact
    23941 cgaagagcat tttaagcagt tcgaagcatc cccagtgtta tttgtcggct ccggtgtgtc
    24001 tcgtcgttat cttggtgtgc catgttggca agaccttttg aagcattttg ctgaggcaat
    24061 tggggaaaat catataaaat taaaaacaaa atctaatggt gacttacccg aatatgcaca
    24121 attattggtg tctgcatatg ctgagaaatg gtgggatact gaagaaggac aattagcatt
    24181 aagtgaaaaa gaacaggaga aaactttcat taacga
//
LOCUS       DAJTNN010000049        23788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTNN010000049 DAJTNN010000000
VERSION     DAJTNN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.049"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            324..551
                     /gene="feoA"
                     /locus_tag="OO150_001724"
     CDS             324..551
                     /gene="feoA"
                     /locus_tag="OO150_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5515905.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            568..2889
                     /gene="feoB"
                     /locus_tag="OO150_001725"
     CDS             568..2889
                     /gene="feoB"
                     /locus_tag="OO150_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5515906.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2889..3125
                     /gene="feoC"
                     /locus_tag="OO150_001726"
     CDS             2889..3125
                     /gene="feoC"
                     /locus_tag="OO150_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5515907.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3328..4206
                     /gene="rpnA"
                     /locus_tag="OO150_001727"
     CDS             3328..4206
                     /gene="rpnA"
                     /locus_tag="OO150_001727"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5515908.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMGCLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(4235..5005)
                     /gene="bioH"
                     /locus_tag="OO150_001728"
     CDS             complement(4235..5005)
                     /gene="bioH"
                     /locus_tag="OO150_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5515909.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            5043..5726
                     /gene="gntX"
                     /locus_tag="OO150_001729"
     CDS             5043..5726
                     /gene="gntX"
                     /locus_tag="OO150_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5515910.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5785..6360
                     /gene="nfuA"
                     /locus_tag="OO150_001730"
     CDS             5785..6360
                     /gene="nfuA"
                     /locus_tag="OO150_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5515911.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6721..8037
                     /gene="gntT"
                     /locus_tag="OO150_001731"
     CDS             6721..8037
                     /gene="gntT"
                     /locus_tag="OO150_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5515912.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGLDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8082..10166)
                     /gene="malQ"
                     /locus_tag="OO150_001732"
     CDS             complement(8082..10166)
                     /gene="malQ"
                     /locus_tag="OO150_001732"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5515913.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(10176..12569)
                     /gene="malP"
                     /locus_tag="OO150_001733"
     CDS             complement(10176..12569)
                     /gene="malP"
                     /locus_tag="OO150_001733"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5515914.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLQDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEISEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13181..15886
                     /gene="malT"
                     /locus_tag="OO150_001734"
     CDS             13181..15886
                     /gene="malT"
                     /locus_tag="OO150_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR5515915.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIATVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(15929..16945)
                     /gene="rtcA"
                     /locus_tag="OO150_001735"
     CDS             complement(15929..16945)
                     /gene="rtcA"
                     /locus_tag="OO150_001735"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5515916.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(16949..18175)
                     /gene="rtcB"
                     /locus_tag="OO150_001736"
     CDS             complement(16949..18175)
                     /gene="rtcB"
                     /locus_tag="OO150_001736"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5515917.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18364..19962
                     /gene="rtcR"
                     /locus_tag="OO150_001737"
     CDS             18364..19962
                     /gene="rtcR"
                     /locus_tag="OO150_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5515918.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWHPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(19944..20702)
                     /locus_tag="OO150_001738"
     CDS             complement(19944..20702)
                     /locus_tag="OO150_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5515919.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(20719..21549)
                     /gene="glpG"
                     /locus_tag="OO150_001739"
     CDS             complement(20719..21549)
                     /gene="glpG"
                     /locus_tag="OO150_001739"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5515920.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIIAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(21594..21920)
                     /gene="glpE"
                     /locus_tag="OO150_001740"
     CDS             complement(21594..21920)
                     /gene="glpE"
                     /locus_tag="OO150_001740"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5515921.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22110..23615
                     /gene="glpD"
                     /locus_tag="OO150_001741"
     CDS             22110..23615
                     /gene="glpD"
                     /locus_tag="OO150_001741"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5515922.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
BASE COUNT         5559 a         6495 c         6016 g         5718 t
ORIGIN      
        1 accaaactca taatatattc cggcaatatt tatcacttca ttaacaactg aaaccttaat
       61 taaacattag ccagtccggg taattcacta ttcgaattat attttcgctg cgatataacc
      121 ttgagccaca tcaacattga gtcagattat tattcaaacc aacattcgca cacattttaa
      181 gtattgctga tagaaaccat tctcattatc attgtgttat tgattattta atctctcctt
      241 tgttggcaaa tcatctggtc tcatgtcgct gtcaaacgcc ccatgaggta gttatccagt
      301 taatgagaaa caagtaggca cctatgcaat acactccaga tactgcgtgg aaaatcactg
      361 gcttttcccg tgaaatcagc ccggcatatc gccaaaaact gctttctctt ggcatgttac
      421 ctggctcctc ttttaatgtg gtgcgcgtcg ctccactcgg cgaccccatt catatcgaaa
      481 cccgtcgtgt gagcctggta ttacgcaaaa aagatctggc cttattagaa gtggaagcgg
      541 tttcctgtta atacggtgat aacaacaatg aaaaaattaa ccataggctt aattggtaat
      601 ccaaattctg gcaagacaac cttatttaac cagctcactg gcgcacgtca gcgtgtaggt
      661 aactgggctg gcgttaccgt cgaacgtaaa gaagggcaat tctccaccac cgatcatcag
      721 gtcacgctgg tggacctacc cggcacctat tctctgacca ccatctcatc gcagacctcg
      781 ctcgatgagc aaatcgcctg tcactacatt ttgagtggcg acgccgacct gctgattaac
      841 gtggtggatg cgtctaacct tgagcgtaac ctgtacctga cgctacaact gctggaactc
      901 ggcattccct gcattgtggc actgaacatg ctcgacattg ccgagaagca aaatattcgt
      961 attgaaattg atgctctgtc ggcgcgtctg ggctgtccgg tgatcccgct ggtttcaacc
     1021 cgtggtcgcg gtattgaagc gctcaagctg gcgattgatc gctataaagc taacgagaat
     1081 gtggaactgg tgcattacgc acagccgctg ctcaacgaag cagattcact ggcaaaagtg
     1141 atgccttccg acatcccgct gaaacaacgt cgctggctgg gcctgcaaat gctggaaggc
     1201 gatatctaca gccgcgccta cgccggtgaa gcgtcgcagc atctggatgc cgccctcgcc
     1261 cgtctgcgta atgagatgga cgatccggcg ctgcacattg ccgatgcgcg ttaccagtgc
     1321 attgctgcca tctgtgatgt ggtaagcaac accctgacgg cagaacccag ccgtttcacc
     1381 actgcggtag ataaaatcgt gctcaaccgt ttcctcggtc tgccgatttt cctctttgtg
     1441 atgtacctga tgttcctgct ggctatcaac atcggcgggg cgttacagcc gctgtttgac
     1501 gtcggctccg tggcgctatt tgtgcatggt attcaatgga ttggctacac gctccacttc
     1561 ccggactggc tgactatctt cctcgcccag ggcctgggtg gcggcattaa caccgtgctg
     1621 ccactggtgc cgcagattgg catgatgtac ctgttcctct ccttccttga ggactccggg
     1681 tatatggcgc gtgcggcgtt tgtgatggac cgtctgatgc aggcgctggg cttgccgggg
     1741 aaatcctttg tgccgctgat cgtcggtttc ggttgtaacg taccgtcggt aatgggtgca
     1801 cgtacgcttg atgcaccgcg tgaacgtctg atgaccatca tgatggcacc gtttatgtcc
     1861 tgcggcgcgc gtctggctat cttcgcagta ttcgcggctg ccttcttcgg gcagaacggt
     1921 gcgctggcgg tcttctcgct gtatatgctg ggtattgtga tggcggtgct gactggcctg
     1981 atgctcaagt acaccatcat gcgcggtgaa gcgacgccgt ttgtcatgga gttgccggtc
     2041 tatcatgtac cacacgttaa aagcctgatt atccagacct ggcagcgtct gaaaggcttc
     2101 gttctgcgtg ctggtaaagt gatcatcatc gtcagcattt tcctgagcgc tttcaacagc
     2161 ttctcgctga gcgggaaaat cgtcgataac atcaacgact cggcgctggc gtccgtcagc
     2221 cgggtgatca ccccggtctt caagccaatt ggcgtgcatg aagataactg gcaggcaacg
     2281 gttggcctgt ttacaggtgc catggcgaaa gaagtggtag tgggtacgct caacaccctc
     2341 tacaccgcag aaaatattca ggacgaagag ttcaatccgg cagagtttaa cctcggtgaa
     2401 gagctgttca gtgcgataga tgaaacctgg cagagcctga aagacacctt cagccttagc
     2461 gtactgatga accccattga agccagcaaa ggcaacggcg aaatgggtac cggggcgatg
     2521 ggcgtgatgg atcagaaatt cggtagcgca gcagcagctt acagctacct gattttcgtc
     2581 ctgctgtatg taccatgtat ctcggtgatg ggagccatcg cccgtgaatc aagccgtggc
     2641 tggatgggct tctccatcct gtgggggctg aatatcgctt actcgctggc aacattgttc
     2701 tatcaggtcg ccagctacag tcagcaccca acttacagcc tggtgtgcat tctggcggtt
     2761 atcctgttta acatcgtggt tatcggtctg ctgcgccgcg cgcgtagccg ggtggatatc
     2821 gaactgctgg caacccgcaa gtcggtaagc agttgctgcg cagccagcac caccggtgat
     2881 tgccattaat ggcttcactt attcaggtgc gcgatttgct ggcgttacgg ggccgtatgg
     2941 aagcggccca gataagccag acattgaaca ctccacagcc aatgattaac gccatgctgc
     3001 aacaactgga aagtatgggc aaagccgtgc ggattcagga agaacctgac ggctgcctct
     3061 ctggcagttg taaaagctgc ccggaaggaa aagcctgtct gcgcgagtgg tgggcgcttc
     3121 gttaacctta ctccatcgcc tgttttggaa agcccggtat gcgtctgcat ccgggctttt
     3181 ttgcgtgcgg ctttccataa aaatgcaact cttgcagcac ggcgtaagtt cctttgaaag
     3241 catctcgcag ggatgaaaac tcgctaatac acaggtgtgg agtggcgcgt agagtcgcgg
     3301 cattcaaaca acaggtgaag gaacgccatg agcaaaaagc agagttccac cccacacgat
     3361 gcgctgttca aactcttttt acgccaaccg gacacggctc gtgattttct tgcgtttcat
     3421 ttaccggcac ccattcacgc gctttgtgat atgaaaaccc tcaagctgga gtcgagcagc
     3481 tttattgatg acgatctgcg tgaaagctat tccgatgtgc tgtggtcggt gaaaacggaa
     3541 caaggaccag gatacatcta ttgtctgatt gaacatcaaa gcacctcaaa caaactgatc
     3601 gcatttcgca tgatgcgtta cgctattgcc gcaatgcaaa atcaccttga tgctggatac
     3661 aaaacgttgc cgatggtggt gccattgttg ttttaccacg gtattgaaag cccctatccc
     3721 tattcgctgt gttggctgga ttgtttcgcc gatcccaaac tggcaaggca gctttatgcc
     3781 tccgcatttc cgctgattga tgtcaccgtc atgcctgatg atgaaatcat gcagcaccga
     3841 cgtatggcgc tgctggagtt aattcaaaaa catattcgtc aacgcgacct gatggggcta
     3901 gtagagcaaa tgggctgctt attaagtagt ggatacgcta atgacagaca aatcaaaggg
     3961 ctgtttaatt acatactgca aaccggcgac gcggtacgtt ttaacgattt tatcgacggc
     4021 gttgccgaac gttcaccgaa acacaaggag agtttaatga caattgcgga aagattgcgg
     4081 caggaggggg aacaatccaa agccctgcat atagccaaaa taatgcttga atccggagtc
     4141 cctcttacag acatcatgcg ctttaccggg gtgtcagaag aagagttggc tgcggcgagc
     4201 cagtaaattt ctgtctcgcc atttcaaaag ttgcctacac cctctgcttc aacgccacca
     4261 gcaggtgaca aaactcgacc ggatgcgaaa taaacggcgc atgggccgct ttggcgaaga
     4321 tatatgattt gctgcgaggc cagagtttat ccagcatcgg caccactttg cgcggcacca
     4381 gaccatcgag atagccatac aatcgcaaaa acggcatggg cacgttttgc agcggctgac
     4441 ggagatcaac cgttttcagg atttccagcc cgccattaag cacgtcaacc tccggcatcg
     4501 gtaacgccag aacggttttc ttcaacgccc gcgcatcctg gcgcgccgtt tcagtcccca
     4561 tggtttgtaa cgccaggaac cgctccactg tacgctgaaa atcatcactg agttgctgct
     4621 gaaatcccgc cagcacgtcc ggttttatcc ccggccaatc gtcacgagca ctaaaacaag
     4681 gtgacgacgc cacggtgacc agcgcctgaa cacgctcggg atgggttaac gcaatctggc
     4741 ttgccaccag cccgcccaga ctccagccta accaaatggc tttatcaggt gcctgttgca
     4801 gcacggcttc ggccatatca gcaagtgaca gcgcaccaaa tccctggctg cgcccgaagc
     4861 cgggcagatc aacaaggtgc agcgtaaaat gcgagctaag ttcctcgtca atgcaacgcc
     4921 acacttcggc attcagtccc catccgtgca gcagcacaag atgaacattc ccctgacctt
     4981 tggtctgcca ccagatgtta ttcatccgct attgttctct tttgacttac aaggatgaac
     5041 atatgctaac agtaccggga ttatgctggc tatgccgaat gccgctggcg ttaggtcatt
     5101 gggggatttg ttcggtctgc tcacgcgccg cccgtacaga taaaacgtta tgcccacaat
     5161 gtggattacc cgccacacac tcacatcttc cctgcggtcg ctgcctgcaa aaaccaccgc
     5221 cctggcaaag actggtcacg gttgccgact atgcgccgcc gttaagtccg cttatccacc
     5281 agcttaaatt ttcccggcgc agtgaaatcg ccagcgccct gtcacgtctg ttactattgg
     5341 aagtcttaca cgctcgtcgc accaccgggt tgcaattgcc ggatcgcatc atcagcgttc
     5401 cgttatggca gcggcgtcac tggcgtcggg gatttaatca gagcgatttg ctgtgtcagc
     5461 cgttatcacg ctggttgcac tgccgatggg atagcgaagc cgtcacacga acacgggcca
     5521 ctgcgaccca gcattttctc agtgcccggc tacgcaagcg caacctgaaa aatgcctttc
     5581 gtcttgaatt gcccgtgcaa ggtcgccata tggtgattgt ggatgatgtc gttaccaccg
     5641 gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa tggtgcggcg actgtccagg
     5701 tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatag gcgtattata accaactaaa
     5761 atagtcaact attaggccat tactatgatc cgtatttccg atgctgcaca agcgcacttt
     5821 gccaaactgc tggcaaacca ggaagaaggg acacaaatcc gcgtatttgt gattaaccct
     5881 ggcacgccta acgctgaatg tggtgtttct tattgtccgc cggatgccgt ggaagccacc
     5941 gacactgccc tgaaatttga cctgctgacc gcgtatgttg atgagttaag cgctccttac
     6001 ctggaagatg cagagatcga ttttgttacc gaccagttgg gttctcagct aacactgaaa
     6061 gcgcctaacg ccaaaatgcg taaagtggca gacgatgcac cactgatgga gcgcgtggag
     6121 tatatgctgc agtcgcagat caacccacag cttgctggtc acggtggccg cgtttcactg
     6181 atggaaatca ccgaagacgg ttacgccatt ctgcaatttg gcggcggctg taacggttgt
     6241 tccatggtcg atgtgacgct gaaagaaggg atcgagaagc agctgctaaa cgaattcccg
     6301 gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg gcgaacactc ctactactaa
     6361 gttatcccct cttttgtaga ttgcccgatg cgacgctaaa gcgtcatttc gggcctacgt
     6421 tgaacatgtg ccagacgtgg gcgcagcatt gctttggcaa atccctccct tcgtttctcc
     6481 gcgataatat gaccaacctc tcataattta aatttactcc gctctggtga ttctcaaacg
     6541 ccagatgtta ccggtatcat tcacatgggt accaaacata ctcctgacat ctgactacaa
     6601 taattagttt tagtgggtat cagtcgtggt gccgcaatat ctctgttccc gattgggata
     6661 attagagttt gtcgtcagaa aattgacgtt acccgcaaca aatgaaaggc caggtaaatc
     6721 atgccattag tcattgttgc tatcggtgta atcttgttgt tgctcctgat gatccgtttc
     6781 aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg ctgttggatt aatgcaaggg
     6841 atgccgctgg ataaagttat tggctccatc aaagccggtg tcggcgggac gctcggtagc
     6901 cttgccctga tcatgggttt tggcgcgatg ctgggcaaaa tgctggcaga ctgcggtggc
     6961 gcacaacgta tcgccaccac cctgattgcc aaatttggta aaaaacacat ccagtgggcg
     7021 gtggtactga ccggttttac cgttggtttt gccctgttct atgaagtggg ctttgtgctg
     7081 atgctgccgc tggtgtttac catcgcggct tctgcgaata ttccactgct gtatgttggt
     7141 gtaccaatgg cagctgcact gtctgtgacc cacggcttcc tgccaccgca tccgggtccg
     7201 actgcgattg ccaccatttt caatgccgac atgggtaaaa ccctgctgta cggtactatt
     7261 ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg ctcgcgtgtt gaaaggtatc
     7321 gataagccga tccctgaagg tctgtacagc gcgaaaacct tcagcgaaga agagatgccg
     7381 agctttggcg tcagcgtctg gacctctctg gtgccggtag tgctgatggc gatgcgtgcg
     7441 attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga gttcctcggt
     7501 gacccggtaa tggctacgct gattgccgtg ctgattgcga tgttcacctt tggtctgaac
     7561 cgtggtcgtt caatggatca gattaacgac acgctggttt cttccatcaa aatcattgcg
     7621 atgatgctgt tgatcatcgg tggtggcggt gcgttcaagc aggtgctggt agacagcggc
     7681 ttggacaaat acattgcttc catgatgcac gaaaccaaca tttctccgct gctgatggcc
     7741 tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg caaccgttgc ggcaatcact
     7801 gcgggtggta tcgcggcacc gctgattgca acgacgggtg ttagcccgga gctgatggtt
     7861 attgcggtgg gttccggtag tgtgattttc tctcatgtga acgatccggg cttctggctg
     7921 ttcaaagagt actttaacct gactatcggc gagaccatca aatcctggtc gatgctggaa
     7981 accattatct cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt gatttgataa
     8041 cactgactgc cggatgcatc gcatccggca tcaaccgcac tctactgctt cttcgctgca
     8101 gctctgcgcc gtctgtccaa atccttcagc aacttattca cgccatcatc ggcaaacatc
     8161 gtctcaagcg ttgcggaaag cttgcgtcgc cagtttttat actggtaact ggtgccagga
     8221 atattcaccg gttcggccat atccagccag tcttccggct gtagtcctaa cagagcactg
     8281 ttactgtcgg caatgtagcg ctgcaaacca cgattaagcg tcggcgtcat cgacatcaac
     8341 gatgccttat gcccggcacg ttttggcaga caaccatatt tatgcagtgc atccagcagc
     8401 ccttgcttcg ccagttcgcg atcctgatac agaccgcgca gtaccacttc atccggatac
     8461 agccccaggg ttttgcccaa cgttagatcc ccgctctccc agtaaccgcg cagcgtaggc
     8521 aggtcatgtg tcgccgcaac cgccatcgac tgctccggat acgctttcgg tgcacggaac
     8581 gtcttctcgt ggtcgttttc gaaatagagc actttgtagg agtacacacc gctgctgcgc
     8641 agcttaccga caatctctac cggtacggta ccgagatctt caccaatcac catacagcga
     8701 tgacgtttac tttcgagtgc cagaatcgag agcagatcat ccaccggata gtgaacatac
     8761 gcgccctgat ctgccgtctc gccatacggt atccaccaca aacgcagcat cgacatcaca
     8821 tggtcaattc gtaatgcgcc gcagttttgc atattggcac gcaacagctc gataaacggt
     8881 tcataggcac gcgcggtgat gatatgcggg tccattggcg gtaatcccca gttctgcccc
     8941 aacgggccga ggatatccgg cggcgcgcca accgacgctt tcaggcaata tagttcacgg
     9001 tcacaccagg tttccgcccc accttccgct acgccaaccg ccagatcacg atacaagcca
     9061 atcggcattt catagccctg gcttatctcc cagcaggcgg caaactggct gtaagccagc
     9121 cactgcaacc agagataaaa atcgacgtca tcacgatgtt cttcgcagaa ctgacgcact
     9181 tctggtgaat ccacgttctg atacatctct ggccatgcag gccagcccca gcgcatttcg
     9241 tcctctttca cttgctgggc atgtagcgca tcaaaggctg cctgccagaa caggctgtcg
     9301 ccctgctctg caacaaactg gcgaaacgcg gtcatctgct catcatcacg ttgcgcgaaa
     9361 cctttccacg ccattcgtaa tgctgtcatt tttagggcgg taaccgtgga gtaatcgacc
     9421 cagtcggcat cgcgcgcctg ttgcagcgtc tgttgcgtgg tcggcaactg ccaccaggcc
     9481 tgagcctctt cgctaagatg gaaatcttca acggcgttaa cgtcgatata aatcacattc
     9541 agccaacggc gagaagacgg actgtatggg ctggcactct ccggatttgc cggatagagc
     9601 gcatgaatcg ggttcaggcc gatgaacgac ccgccacgtt ttgccacatc caccagcatc
     9661 gctttgagat cgccaaaatc cccaataccc cagttttttt ccgatcgcag cgtataaagc
     9721 tgaacgcagg caccccacag cttttgttta ttcagcaacg cctgcggttc gtaacagcgt
     9781 ttcggggcga caatcacccg gcaatgcgcg cgctggtcgt cctgggtgag tgtcagcgtg
     9841 tgataacctt ccggcagctt cgtcggtaga ttgaacgctt tgccccccgt tacatggcct
     9901 ttgtactgtg ttccttcttc ggtggtcagc agccagctat attcgccgct gccctccacc
     9961 accatcggca tttttttgcc gctggtataa accatgacat tcgggactgg cgttaccgcc
    10021 actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac gccgtttggt ttcggcgcta
    10081 atcgactgcg gtttaccgtg ggcattgatg taattggggc taatccccgc cgccagcgcg
    10141 gcattatcca gacgtttgct ttccatcgag cttccttagc gttttgcctg ccagatacga
    10201 gcctgataat cgcgaataga gcgatccgag ctaaacatac cgcagcgggc ggtattgagg
    10261 atcgccgcgc gagtccaggc ctcctggtcg cggtacagca catccacctg cttttgtgcc
    10321 tcaacatagg ctgcgaaatc cgccatcacc agatacggat cgccgccttg tttgccgata
    10381 ctgtgcagca tctggtcgaa ggcatgctta tcgccgtcgc tgtatttacc gctttccagc
    10441 tctttcaata ctgcgtccag caccttatct ttcttccgcc atttcaccgg gtcgtagcct
    10501 ttggccagaa ttgccttcac ttgttccacg gtatgaccaa aaataaagat attttcttca
    10561 ccgactttct cggagatttc aacgttcgca ccatccagcg taccgacagt aagcgcacca
    10621 ttgagcgcca gtttcatatt gccggtaccg gaagcttctt tacctgcagt cgaaatttgt
    10681 tcggagatat ccgccgccgg gatcagtttt tccgccgccg aaacgcaata atcctgcagg
    10741 aacaccacct tcaacttatc gccaaccagt ggatcgttgt tgatcacgtc agccactttg
    10801 ttgatcgcaa agataatatt cttcgccagg tagtagcccg gtgccgcttt cgcgccgaag
    10861 aggaagacgc gcggtacgcg atcagcctgc gggttttcac gaatttcttt gtacaacgcc
    10921 agaatatgca gcagattcag gtgctggcgt ttgtactcat gcagacgttt aatctgaata
    10981 tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa ctttcacaaa ctccgccaga
    11041 cggactttat tcgcctgctt gatctcgcga tattgctgac ggaatttcgc atcatcagcg
    11101 aatttttcca gattgatcag ctgatcgaga tcgttagccc actctttttg cagtaattta
    11161 tccaacagag ccgccagtgc agggttgcac tgtttgatcc agcgacgtgg ggtaataccg
    11221 ttggtgacgt tatggaattt gttcggccat agctggtgat attccgggaa cagatctttc
    11281 accaccagat ccgagtgcag cgccgcaaca ccgttcaccg cgaaaccgcc aaccacacac
    11341 aggttcgcca tatgcacttg tttatcgtgc accaccgcca gtttggccca cactttttca
    11401 tcgcccggcc aggttttctc caccagcgtt ttaaagcgag tattaatttc gttaataatc
    11461 tgcatatggc gcggcagtaa gcctttcacc agtttcacat cccagcgttc cagcgcttct
    11521 ggcatcaggg tatggttggt gtaagcgaaa gttttgctgg taatggccca ggcgtcatcc
    11581 cagctcatct ggtgctcatc gatcagcacg cgcagcagtt ctggaatcgc gatagttggg
    11641 tgggtatcgt tcagctgaat aacttcataa tccgccagtt cgtgcagttt acgccccgcc
    11701 agatgatggc gacgcaaaat atccgctacc gaacaggcac actggaagta ctgctgcatc
    11761 aagcgcagct ttttaccggc agtatggttg tcgtttggat agagaacttt ggtcagtttt
    11821 tccgcattga tgccctgctg ttcggcacgc aggaaatcac cgtcgttaaa tttagtcaga
    11881 tcaaacggat gcgcgtgcgt cgcctgccac agacgcagcg gctgcgccac gccattacga
    11941 tagccgacaa cggggagatc ccacgcttga ccggtaatgg taaactccgg ctcccagcgt
    12001 ccgtctttcg tcactttacc gccaatccct acctgcacat ccagtgcttc gttgtggcgg
    12061 aaccacgggt agttactacg atgccagtca tccggcgctt caacctgttt gccatcgaca
    12121 aaagactggc ggaacaaacc atattgatag ttcagaccgt aacccgtcgc agactgaccg
    12181 atagttgcca ttgagtcgag gaagcacgcc gccagacgtc ccagaccacc gttacccagc
    12241 gccgggtcga tctcttcttc cagcaggtcc gtcagattga tgtcataagc cttcaacgaa
    12301 tcctgtactt cctgatacca gccgagattc aacaggttgt tgcccgtcag gcgaccaatc
    12361 aaaaactcca ttgagatgta gttaacatgt cgctgattcg ccaccggctt ggcgaatggc
    12421 tgagcacgca gcatttcggc cagtgcttca ctcactgcta gccaccactg gcgaggagtc
    12481 atttcagccg cagaatttaa gccataacgc tgccactgac gtgaaagcgc ttcctgaaat
    12541 tgcttatcgt taaaaatagg ttgtgacata ggagttccac ttttcttaga ttttcaacac
    12601 aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc tcatcctgcg ggggattagg
    12661 cagggaggag ttgcggggat gagcaaggaa atgtgatctc aaccacttaa agctagtgca
    12721 aaccacagga ttagcagcaa atcaatgcaa tacagcgcag aaaatctgta tctaagtgca
    12781 aaaaatggcc gttgcgtatt ttcaaaaagc ggaaggtaac tctataaatt aagtaaagga
    12841 gtgaaacagt ttcacaagta aaatatccag tgtgctccat ctcattctta atagatttat
    12901 taagatcatc tttttagatg gcactttcat caggaatgaa gaagaaaccc ttgcttaaat
    12961 gaatctgatg aacataaggg aaaccagtat tcacgctgga tcagcgtcgt tttaggtgag
    13021 ttgttaataa agatttggaa ttgtgacaca gtgcaaattc agacacataa aaaaacgtca
    13081 tcgcttgcat tagaaaggtt tctggccgac cttataacca ttaattacga agcgcaaaaa
    13141 aaataatatt tcctcatttt ccacagtgaa gtgattaact atgctgattc cgtcaaaact
    13201 aagtcgtccg gttcgactcg accataccgt ggttcgtgag cgcctgctgg ctaaactttc
    13261 cggcgcgaac aacttccggc tggcgctgat cacgagtcct gcgggctacg gaaagaccac
    13321 cctcatttcc cagtgggcgg caggcaaaaa cgatatcggc tggtactcgc tggatgaagg
    13381 tgataaccag caagagcgtt tcgccagcta tctcattgcc accgtgcagc aggcaaccaa
    13441 cggtcactgt gcgatatgtg agacgatggc gcaaaaacgg caatatgcca gcctgacgtc
    13501 actcttcgcc cagcttttca ttgagctggc ggaatggcat agcccacttt atctggtcat
    13561 cgatgactat catctgatca ctaatccagt gatccacgag tcaatgcgct tctttattcg
    13621 ccatcaacca gaaaatctca ccctggtggt gttgtcacgc aaccttccgc aactgggcat
    13681 tgccaatctg cgtgttcgtg accaactgct ggaaattggc agtcagcaac tggcatttac
    13741 ccatcaggaa gcgaagcagt tttttgattg ccgtctgtca tcgccgattg aagccgcaga
    13801 aagcagtcgg atttgcgatg acgtttccgg ttgggcgacg gcactacagc taatcgccct
    13861 ctccgcccgg cagaataccc actcagccca taagtcggca cgccgcctgg cgggaatcaa
    13921 tgccagccat ctttcggatt atctggtcga tgaggttttg gataacgtcg atctcgcaac
    13981 gcgccatttt ctgttgaaaa gcgccatttt gcgctcaatg aacgatgcgc tcatcacccg
    14041 tgtgaccggc gaagaaaacg ggcaaatgcg tctcgaagag attgagcgtc aggggctgtt
    14101 tttacagcgg atggatgata ccggcgagtg gttctgctat cacccgctgt ttggcaactt
    14161 cctgcgccag cgctgccagt gggaactggc ggcggagctg ccggaaatcc accgtgccgc
    14221 cgcagaaagc tggatggccc agggatttcc cagcgaagcg attcatcatg cgctggcggc
    14281 aggcgatgcg ctgatgctgc gcgatattct gcttaatcac gcctggagtt tgttcaacca
    14341 tagcgaactg tcgctgctgg aagagtcgct taaggccctg ccgtgggaca gtttgctgga
    14401 aaatccgcag ttagtgttat tgcaggcgtg gctaatgcaa agccaacatc gctatggcga
    14461 agttaacacc ctgctagccc gtgctgaaca tgaaatcaag gacatcagag aaggcaccat
    14521 gcacgcagaa tttaacgctc tgcgcgccca ggtggcgatt aacgatggta atccggatga
    14581 agcggaacgg ctggcaaaac tggcactgga agagctgcca ccgggctggt tctatagccg
    14641 cattgtggca acctcggtgc tgggtgaagt gctgcactgc aaaggagaat tgacccgctc
    14701 actggcgcta atgcagcaaa ccgaacagat ggcacgccag cacgacgtct ggcactacgc
    14761 cttatggagt ttaatccagc aaagtgaaat tctgtttgcc caagggttcc tgcaaaccgc
    14821 gtgggaaaca caggaaaaag cgttccagct gatcaacgag cagcatctgg aacagctgcc
    14881 gatgcatgag tttctggtgc gcatccgagc acaactgtta tgggcctggg cacggctgga
    14941 tgaagccgaa gcgtcggcgc gtagcgggat tgaagtcttg tcgtcttatc agccacagca
    15001 acagcttcag tgcctggcaa tgttgattca atgctcgctg gcccgtggtg atttagataa
    15061 cgcccgtagc cagctgaacc gtctggaaaa cctgctgggg aatggcaaat atcacagcga
    15121 ctggatctct aacgccaaca aagtccgggt gatttactgg caaatgaccg gcgataaagc
    15181 cgccgctgcc aactggttgc gtcatacggc taaaccggag tttgcgaaca accacttcct
    15241 gcaaggtcaa tggcgcaaca ttgcccgcgc acaaatcttg ctgggcgagt ttgaacctgc
    15301 ggaaattgtt ctcgaagaac tcaatgaaaa tgcccgcagt ctgcggttga tgagcgatct
    15361 caaccgtaac ctgttgctgc ttaatcaact gtactggcag gccggacgta aaagtgacgc
    15421 ccagcgcgtg ttgctggacg cattaaaact ggcgaatcgc accggattta tcagtcattt
    15481 tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag ctgattcaac tcaatacgct
    15541 gccggaactg gaacagcatc gcgcgcagcg tattctgcga gaaatcaatc aacatcatcg
    15601 gcataaattc gcccatttcg atgagaattt cgttgaacgt ctgctaaatc atccggaagt
    15661 gccagaactg atccgcacca gcccgttgac ccaacgtgaa tggcaggtcc tggggctgat
    15721 ctactccggt tacagcaatg agcaaattgc gggcgagctg gaagtggcgg caaccaccat
    15781 caagacgcat atccgcaatc tgtaccaaaa actcggcgtc gcccaccgtc aggccgcggt
    15841 acaacacgcg cagaaactgc tgaagatgat gggatatggg gtgtgagttt agccggataa
    15901 tgcattggat ccggtttaca tctttacatc attcaatact cacccgcgtt acgccatctg
    15961 tttctatcaa accaaaccgc accggcaaga aacgctccac cacggcgata ttggtcagca
    16021 gatggcatga aggatgggcg accgtaaatt ctcccgcgcc cgccagcgcc atcggtagca
    16081 ccagctggtc ggcgagatat tcccccactg ccgccgggct tgccaggtag cgtttcacct
    16141 ctttcactaa ctgcgcggct accacctcgg cactgacgcg cttttcaccg acgacgaaaa
    16201 agcgttcagt gatattttca ctttcgactt ccagcgagac ggtattaccc ggcccctgat
    16261 cgcgcggcag gttatgaata ttctgttcat gcagggaaaa actccccgcc agtgtagcga
    16321 tttcacgctc agcaacatgg cgcggcacac cagctaatag aacttctcca cgcatctgca
    16381 caatgttccc gcgctcgcca agttgcaagg tgttaaacga tgccacgggc gagacttccg
    16441 ttgccaccac gccgcctccg gcaggataaa aaccgtgacg taacagcgtg gtttgctgat
    16501 gaattcctat tttcgccagc agcggctcca gcacccggcg gataaaatcc gcaggcggcg
    16561 ccgacgggtt atcggtgcct ccgctcactt caacacgcga aggtccatcg gcaaaccaca
    16621 gcgcgggcag caccgtttgc agcaccagcg tacagcttcc ggcactaccg atagcaaagc
    16681 ggtactcgcc gccgcgcacg gcgccaggcc ggaagaccag acgctgcgac cccagctccg
    16741 cgccttccac cgttgcccta caaatttccg cagccgcttt taccgcggtc agatgctggc
    16801 gcaacagccc cggtttcgcc cgcccggcac gaatgctggt gatggtaaat ggctggccgg
    16861 ttatcatcga caggctcagc gccgagcgca gaatctgccc gccaccttcg ccctgtgcgc
    16921 catccagcgc aatcatcctt ttcatcattt atccttttac gcacaccacc tgacgcaggg
    16981 tatagataac ttccaccaga tcgctttgtg ccgtcatcac cgcatcaata tctttatacg
    17041 ccatcgggat ttcgtcgatc acttcggcat ctttacggca ttccacatgc gcggtggcac
    17101 gaatttgatc ttccacgctg aacagttttt tcgctttagt tcggctcatt acccgcccgg
    17161 caccgtggct gcacgaacag aacgactctt catttcccag cccacggacg ataaagcttt
    17221 ttgctcccat cgaaccggga ataattccat attgaccagc acgcgcagac accgcgcctt
    17281 tacgcgtcac gtagatctct tcaccaaagt gctgttcttt ttgcacatag ttgtggtgac
    17341 agttgatctc ttccatcgcc agcgtttgtg gctgtctgac cgttttctgc gtaatgctct
    17401 gcaatgccgt taccacgttt tccatcatca catcgcggtt aaggctggca aaaagctgcg
    17461 cccaggccac ggctttcagg taatcatcaa agtattccgt accttccata aagtacgcca
    17521 gatcacgcga cggcaacgtc tcaagcgttt cctgcatctc tttttgtgcc agatcgataa
    17581 agtaagtccc gatggcgtta ccaattccgc gtgaaccgga gtgcagcata atccacacct
    17641 ggtccgactc atcaaggcag attttaataa agtggttacc ggttcccagc gttcccaggt
    17701 gtttatagtt attggtattc aggaaacggg gatatttttg cgttaaccac tgataaccgg
    17761 cttcaagctc agcccatttc gcatcgacgt tgacaggcgg gttttcccat gcgcctttat
    17821 cacgtttaca acgcccggta gtacgcccgt gcggcacggc cgtttcaatc gcctgacgca
    17881 gctctgccag gttttcaggc aggtcttccg ccgttaacgc ggtacgcagc gcgttcattc
    17941 cacagccaat atccacgccc accgccgccg gaataatcgc ccctttggtc gggatcacgc
    18001 taccaatggt ggaatcttta cccaggtgta catcaggcat taccgcaata tgtttgaaaa
    18061 taaacggcat cttcgccgta ttaataagtt gctgacgcgc atcggcttct accggcacgc
    18121 ctttggtcca catttttacc ggggcatttt cagtggtcag taattcgtaa ttcattttgt
    18181 ttttctcttt tcgttgtttg ctgtcctgat aattgcaacc gtcgtgccag aaaattaaat
    18241 aagtaaatta attttttaat tcattgtttt taaaaagatt atatctttat gtccgtaacc
    18301 ggagatttcc cgcaaagccg atttaccgat aatgaaatat cgtcttttat aaggatatct
    18361 aagatgcgta aaacagtagc ttttggcttt gtcggaacgg tgctggatta tgccgggcgc
    18421 ggcagtcagc gctggtcaaa atggcatccg acactctgtt tatgccagca agaatcgttg
    18481 gtcatcgatc gactggaatt attgcatgac gcccgctcgt gctcgctatt tgaaacgctt
    18541 aaacgcgata tcgccagcgt ttcgccagaa acagaagtgg tgagcattga gattgaactg
    18601 cataacccgt gggatttcga agaggtttac gcctgcctgc atgatttcgc ccgtggttac
    18661 aagtttcagc cagaaaaaga agactattta attcacatca ccaccggcac ccacgtcgcg
    18721 cagatttgct ggtttctgct ggcggaagcg cgttatctac ccgcccggtt gatacaaact
    18781 tcaccaccgc gcaaaaaaga acaaccgcgc ggtgccggtg aagtgacgat tatcgatctc
    18841 gatctaagcc gttataacgc catcgccagc cgctttgccg aggaacgcca gcaaacgctt
    18901 gattttctta agtccggcat tgccacgcgt aacccccact tcaaccgcat gattgagcag
    18961 attgaaaaag tggcgattaa atcccgcgca ccgatcctgc ttaacggccc aaccggtgcg
    19021 ggaaagtcat ttctggcgcg acgtatattt gaattaaaac aggcgcggca tcagtttagc
    19081 ggcgcgtttg tggaagtgaa ctgcgccacc ctgcgcggcg ataccgccat gtcgacgctg
    19141 tttggtcatg tgaaaggcgc gtttaccggg gcgcgggaat ctcgtgaagg tttattacgc
    19201 agcgccaacg gcggaatgtt gttttttgat gagattggcg aactgggcgc agacgaacag
    19261 gcaatgctgc taaaagccat tgaagagaaa accttttacc cgtttggcag cgatcgccag
    19321 gtgagcagtg attttcagct tatcgccgga acggtgcgcg atttgcgcca gctggttgcc
    19381 gaaggcaaat ttcgcgaaga tctgtacgcg cggatcaatc tctggacctt caccctgccg
    19441 ggtctacgcc agcgccagga agatattgaa ccgaaccttg attatgaagt ggagcgccac
    19501 gccacactca ctggcgacag cgtgcgtttt aacaccgaag cgcggcgcgc ctggttggct
    19561 tttgcgacct ctccccaggc aacatggcgc ggtaactttc gcgaactttc tgccagcgtc
    19621 acgcggatgg ccacctttgc cactagcgga cgcatcactc tggacgtggt tgaagatgag
    19681 ataaaccgtc tgcgctataa ctggcaggag agccgcccct ccgcgcttac ggcgttgctg
    19741 ggcgcagagg cagaaaacat cgatctcttc gaccgtatgc aactggaaca cgttatcgct
    19801 atctgccgcc aggcaaagtc gctttctgca gccggacgac agctttttga tgtttcgcgc
    19861 cagggaaaag ccagcgtcaa tgacgcggat cggctacgca aatacctggc gcgttttggt
    19921 ctgacgtggg aagccgtgca ggatcagcac agctccagtt gaatatgatg gtccgtcagc
    19981 acctgcatta cgcttactgg cggcggggcg tcggtgtaga cggcatctac catgctgatg
    20041 ctgcccatat tgaccatcgc gttacggcca aatttcgagt gatcgacaac cagcataacg
    20101 tggcgcgagt tctcaataat ggcgcgcttg gtgcgaactt cgtggtaatc gaactccagc
    20161 agcgagccgt cgctatcgat gccgcttatc cccagaatgc cgaaatcaag gcggaactgg
    20221 gagataaaat cgagcgtcgc ttcgccaatg atcccgccat cgcggctgcg taattcgcca
    20281 ccagcgagaa taatgcggaa atcttctttt accatcaacg tgttagcaac attgagattg
    20341 ttggtgacaa tacgcaaatt gctgtgatta agcagcgcgt gcgccaccgc ttccggcgtg
    20401 gtgccgatat cgataaacag tgtcgagccg ttggggattt gctccgccac tttgcgggca
    20461 atcctctctt tttcttcggt ctgggtggct ttgcgatcgt gccacggcgt gttaaccgaa
    20521 ctggaaggca gcgccgcacc gccatggtga cgcaggatca ggttttgctc cgccagctca
    20581 ttgaggtcgc ggcgaatagt ctgcgggctg acggagaaat gctctaccag ctcttcagta
    20641 ctgacataac cctgctgttt aaccagttcg ataataccgt tgtgacgttg tgtttgtttc
    20701 atttataaat ccctggaatt attttcgttt tcgcgcattg agcgaatcaa caaaagccat
    20761 cgctaaaccc acggctaacc cggcgatgtg tgctccgttc gccatcgaca tcccaaacaa
    20821 atcaaaccat ccggcgataa tccagatcag cgcaaagata attaacccac gttgcaggta
    20881 aatgccactt tgcggatcgc gctcaccacg tagccagacg tagcccatca gcgcatacac
    20941 cacgccagaa agcccgccaa accacggccc gctgaatttt tgctgcacat agccgcttaa
    21001 cagggcacta atgagagtaa taacaattag cttaccgcta ccgaggcgtt tttccaccgc
    21061 accgccgaga taccaccacc agagcaggtt aaagaggata tgcatcagcg agaagtgcat
    21121 taacgcgtgg gtgaagtaac gccagaactc aaatttcagt gttggatcga atggccaggc
    21181 cagccataac atcacttcct gatcgccgag aatttgcatg gcgataaaca ccaccacgca
    21241 ggcgatcatc accacccagg ttaccggacc tgcgcgttca cgcaaggcag caaagaaagg
    21301 ataacggcga taatgcagac cactgccggt atggcccgcc tgccagctgg ccgccagata
    21361 acgcggatct gccgggtttt cgagaaaacg cgccagctcc gcccgcacgc gctcggcctg
    21421 ggactcatcc gccagccaga catcgctttg gttatgttgt tgaatcgtaa ggataacacc
    21481 ctgcgtcgcc atgtaatcaa caaacgcctg cgccacgcgg gggttagcaa aagaggtaat
    21541 catcaacatc gttgctgtcg cttattccac acaaaagggg acagtataaa gcgttacgcg
    21601 ccgtacgcca cctctgcggg aaactgacgc tgccaggctt caaaaccgcc gtcaatgcta
    21661 tagaccacat cgtagccctg ttgcagcaga tactgcgccg cgcctttgct gctattgccg
    21721 tgataacaca tcaccatcac cggagtgtca aagtcgttat cacgcataaa cgcgcccagc
    21781 gtgtcgttgg ttaaatggaa agcctgcacc gcatgtccca ttgcgaaact ctgtggatcg
    21841 cgaatatcga ccagcaccgc ctctttttcc tgcaacttct ggtgcgcgtc ggcaacgtta
    21901 atacattcga acagatccat gcgtctctct ttctttacaa acaagtgggc aaatttaccg
    21961 cacagtttac gtcgaagcgg cagataaacg ccataatgtt atacatatca ctctaaaatg
    22021 ttttttcaat gttacctaaa gcgcgattct ttgctaatat gttcgataac gaacatttat
    22081 gagctttaac gaaagtgaat gagggcagca tggaaaccaa agatctgatt gtgataggtg
    22141 gcggcatcaa tggtgctggt atcgcggcag acgccgctgg acgcggttta tccgtgctga
    22201 tgctggaggc gcaggatctc gcttgcgcga cctcttccgc cagttcaaaa ctcattcacg
    22261 gtggcctgcg ctaccttgag cactatgaat tccgcctggt cagcgaggcg ctggctgaac
    22321 gtgaagtgct gctgaaaatg gctccgcata tcgccttccc aatgcgtttt cgcctgccac
    22381 atcgtccgca tctgcgcccg gcgtggatga ttcggattgg tctgtttatg tacgatcatc
    22441 tgggtaaacg caccagcctg ccgggatcaa ccggtttgcg ttttggcgca aactcagtgt
    22501 taaaaccgga aattaagcgc ggattcgaat attctgactg ttgggtagac gacgcccgtc
    22561 tggtactcgc caacgcccag atggtggtgc gtaaaggcgg cgaagtgctt actcggactc
    22621 gcgccacctc tgctcgccgc gaaaacggcc tgtggattgt ggaagcggaa gatatcgata
    22681 ccggcaaaaa atatagctgg caagcgcgcg gcctggttaa cgccaccggc ccgtgggtga
    22741 aacagttctt cgacgagggg atgcatctgc cttcgcctta tggcattcgc ctgatcaaag
    22801 gcagccatat tgtggtgccg cgtgtgcata cccagaagca ggcctacatt ctgcaaaacg
    22861 aagataaacg tattgtgttc gtgatcccgt ggatggacga attttccatc atcggcacca
    22921 ccgacgtaga gtataaaggc gatccgaaag cggtgaagat tgaagagagt gaaatcaact
    22981 atctgctgaa agtgtataac gcgcacttta aaaaacagct tagccgtgac gatatcgtct
    23041 ggacctactc cggtgtgcgt ccgctgtgtg atgatgagtc cgactcgccg caggctatta
    23101 cccgtgatta cacccttgat atccatgatg aaaacggcaa agcaccgctg ctgtcggtat
    23161 tcggcggtaa gctgaccacc taccgaaaac tggcagaaca tgcgctggaa aaactgacgc
    23221 cgtattatca gggtattggc ccggcgtgga cgaaagagag cgtgctgccg ggtggtgcca
    23281 ttgaaggcga ccgcgacgat tacgcagctc gtctgcgccg tcgctatcca ttcctgactg
    23341 aatcgctggc gcgtcattac gcacgcactt acggcagcaa cagcgagctg ctgctcggca
    23401 atgcgggggc gataagcgac ctcggggagg atttcggtca tgagttctac gaagcggaac
    23461 tgaaatacct ggtggatcac gaatgggtcc gccgcaccga cgatgccctt tggcgtcgca
    23521 caaaacaagg catgtggctg aatgcggatc aacaatctcg tgtgagtcag tggctggtgg
    23581 aatatacgca gcagaagttg tcgctggcgt cgtaaggatg atgcccggtt gaagtgcaac
    23641 cgggctattt ggattcctta gactattcat tgtaaataat tgaataacgg cccctgagct
    23701 aaaatcgcca aacttaagca cgttacccaa tcgccccact tcttcctctg gtttctattt
    23761 tttctgcacc aatggcaata atatccca
//
LOCUS       DAJTNN010000050        23346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTNN010000050 DAJTNN010000000
VERSION     DAJTNN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.050"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..312)
                     /gene="sufE"
                     /locus_tag="OO150_001742"
     CDS             complement(<1..312)
                     /gene="sufE"
                     /locus_tag="OO150_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5515923.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RP"
     gene            complement(325..1545)
                     /gene="sufS"
                     /locus_tag="OO150_001743"
     CDS             complement(325..1545)
                     /gene="sufS"
                     /locus_tag="OO150_001743"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5515924.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAEYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(1542..2813)
                     /gene="sufD"
                     /locus_tag="OO150_001744"
     CDS             complement(1542..2813)
                     /gene="sufD"
                     /locus_tag="OO150_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5515925.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTSLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(2788..3534)
                     /gene="sufC"
                     /locus_tag="OO150_001745"
     CDS             complement(2788..3534)
                     /gene="sufC"
                     /locus_tag="OO150_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5515926.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(3544..5031)
                     /gene="sufB"
                     /locus_tag="OO150_001746"
     CDS             complement(3544..5031)
                     /gene="sufB"
                     /locus_tag="OO150_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5515927.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(5040..5408)
                     /gene="sufA"
                     /locus_tag="OO150_001747"
     CDS             complement(5040..5408)
                     /gene="sufA"
                     /locus_tag="OO150_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5515928.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(5955..6143)
                     /locus_tag="OO150_001748"
     CDS             complement(5955..6143)
                     /locus_tag="OO150_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5515929.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(6243..6653)
                     /gene="menI"
                     /locus_tag="OO150_001749"
     CDS             complement(6243..6653)
                     /gene="menI"
                     /locus_tag="OO150_001749"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5515930.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(6650..9706)
                     /locus_tag="OO150_001750"
     CDS             complement(6650..9706)
                     /locus_tag="OO150_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5515931.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDVLDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPELDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            10095..11207
                     /gene="ydiK"
                     /locus_tag="OO150_001751"
     CDS             10095..11207
                     /gene="ydiK"
                     /locus_tag="OO150_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5515932.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            11393..11500
                     /gene="rprA"
                     /locus_tag="OO150_001752"
     ncRNA           11393..11500
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO150_001752"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            11636..12015
                     /locus_tag="OO150_001753"
                     /pseudo
     CDS             11636..12015
                     /locus_tag="OO150_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            11960..12682
                     /locus_tag="OO150_001754"
                     /pseudo
     CDS             11960..12682
                     /locus_tag="OO150_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12694..13560
                     /gene="ydiB"
                     /locus_tag="OO150_001755"
                     /pseudo
     CDS             12694..13560
                     /gene="ydiB"
                     /locus_tag="OO150_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
     gene            13592..14350
                     /gene="aroD"
                     /locus_tag="OO150_001756"
     CDS             13592..14350
                     /gene="aroD"
                     /locus_tag="OO150_001756"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5515933.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            14496..16091
                     /locus_tag="OO150_001757"
     CDS             14496..16091
                     /locus_tag="OO150_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCR5515934.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFNFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIQELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            16105..17256
                     /locus_tag="OO150_001758"
     CDS             16105..17256
                     /locus_tag="OO150_001758"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR5515935.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(17299..18210)
                     /locus_tag="OO150_001759"
     CDS             complement(17299..18210)
                     /locus_tag="OO150_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5515936.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLSNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            18524..19295
                     /locus_tag="OO150_001760"
                     /pseudo
     CDS             18524..19295
                     /locus_tag="OO150_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            19315..20253
                     /locus_tag="OO150_001761"
     CDS             19315..20253
                     /locus_tag="OO150_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5515937.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLVATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            20309..21598
                     /locus_tag="OO150_001762"
     CDS             20309..21598
                     /locus_tag="OO150_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5515938.1"
                     /translation="MSDDKFDAIVVGADVASSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYSRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            21595..21888
                     /locus_tag="OO150_001763"
     CDS             21595..21888
                     /locus_tag="OO150_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5515939.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            21945..>23346
                     /gene="fadK"
                     /locus_tag="OO150_001764"
     CDS             21945..>23346
                     /gene="fadK"
                     /locus_tag="OO150_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5515940.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYCSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAM"
BASE COUNT         5742 a         5950 c         5841 g         5813 t
ORIGIN      
        1 cggacgcaca tcgaaattga caatatcctg cggcgtcatc tgatcgtaga gaataaatac
       61 gaccgcaata agccctttca caatcgccgc atcgctgtcg ccctgcaatt caataattcc
      121 ctgcgcattc tggcgcatga caatccacac ctgattctgg cagccctgaa tgctattttg
      181 tgggcttcgg tcttcgtcgc gtaattctgg cagacgctgg cccagctcaa taatgtagag
      241 atatttctct tcccagttgg cgcagcgtaa aaaattacgc agcaattttt ctttatccgg
      301 caataaagcc atagtgcctc cctgttatcc cagcagacgg tgaatacgtt gcaggccggt
      361 caccagacga tccacttctt catgagtgtt atacatggcc agcgacgccc gacacatcgc
      421 agggacgttg taataggcca ttaatggcat agcgcagtga tgtccggtac gcacagcaat
      481 gccgtaatta tcgagaaaac tgccaacatc ataggcgtgg tgtttaccga gattaaaagc
      541 aataacgcca agcctgtttt gtgggccata gagagtgaga ttcggtacag attccagctg
      601 tgatagcgcg taatgcatca gattcagttc atactcggct atgttattaa gccccagcac
      661 cgaaacatat tccagcgccg cgccaagacc aatgatgccc ccggtattgg gtgtaccggc
      721 ttcaaaccgc catggtgctt tggtccaggt agtgccttca ctcaggctga cggtggcgat
      781 catagaaccg cccccttccc acggcggcat ctcctgcaac aaggcttctt tgacataaag
      841 aatgccaatc ccggtggggc catacagttt atgcccggaa aacacgtaaa aatcgcaatc
      901 cagcgcctga acatccaccg gatgatgcat caccgcctga gcgccatcca ccagcacttt
      961 tgcgccatgc tggtgcgcaa gcgtgatcat tcccgccagt ggattttctg tgccaagcac
     1021 gttggagaca tgagtaattg ccagcaggcg agttttctca tcaaacagcg tatgcagcgt
     1081 ctccagttgc agcgtaccgt cggggttgag agggatcaca cgcagctctg cgccaacgcg
     1141 tgcgcaaagc atctgccagg ggacaatgtt agcgtggtgc tccatctgac tgatgatgat
     1201 gttatcgccc gcccgcacat tgctgttgcc ccagctattg gcgaccagat tgatcccttc
     1261 cgtcgtgccg cggacgaaca ccagctcttc cgccgaacgg gcattaataa acagcgatgc
     1321 ccgcttgcgc acgttctcca ttttctcggt cgcctgggcg cttaaggtat gaataccgcg
     1381 atgcaccgcc gcgtagccat gacgataaaa ctcggcctcg gcgtcaatca cctggctcgg
     1441 tttctgtgca ctggcggcgc tgtcgagata agccagcggc aaaccgttta cctcacgcga
     1501 aagcaccgga aagtcggccc gcactttgtc gacggaaaaa gtcatcttgc acctcctggc
     1561 agccgttgac cgattcgggc cagcacctgc tgtttaagcc cctcatcacg cagtgcttcc
     1621 gtcagttcgg cagcaaaggc gtaaatgatc atctgctggg catcctgctg attgatcccg
     1681 cgcgagcgca gatagaacat ctgttcatca tcaatacgcc ccaccgtcgc gccgtggctg
     1741 catttcacat catctgcata gatttccagc tgcggtttcg tatccacttc cgccagtttg
     1801 cccatcagca gattattgtt ggtcatctga ccatccgttt tgatggcgtg ctgcgcgacg
     1861 ttgatcaaac cgttaaatac cgcgcggcct ttgtcgctga cgatagtttt gtgcaactgt
     1921 cggctgttac aaaaaccttt attgtgttcc agccaggtac gggtatcaca cacctcgttt
     1981 ttcaccggca tcgccaggct attgatccgc agcgtgctgt tttcgccatt gagttgcgta
     2041 ctggtgttgt gtcgtaacac tgcgccaccc agcaggaaac tatggctaaa tgcggtggca
     2101 tcctcagcca gcaacaaatc gttatgagca aagtggtgac tgagcgggtt ttcaaacgcc
     2161 agcttgatat gctgcaagtg ggcgttcgct gcgacattga tagtgaaccg tgccccggta
     2221 aaatgacgag catcattaag gctgacaaaa tgttcgatca ccgttgcttc ggcaccttcc
     2281 gccagatcca gatgatgccg gtaatgggca gtgttcacct cttcacctgc cacgccctgg
     2341 gtgatataca ttaacagcaa tggctttgcc ggccgttgac cgcgcttcac ggcgatatgc
     2401 gtcacgcttt gtgccaggct ttccgtcaaa tgcagaaaca cttccgcctg aatagcgtcg
     2461 ggtaacccct gacggtcgtc gttaatgctc acttcatatc cgctgccttc aattgcatcg
     2521 ctcagcgcgg gcacgtaacg tccatcgaca aacaccagcc gcacggcgtc taacgttaac
     2581 gctaaggcat cacgctgctg tggggatatc tcccccgcaa tgctgacaaa ctggctattg
     2641 gtcagccctt ccagcgacgt atatttccag ttttcatgtt tacgtgtcgg cagtccggta
     2701 cgcagcaatt gttgtaaatg ctgctgtgct tgcggggagc gttttgtccc ttcagcttca
     2761 aacaagtgat gccactgttg cagcgcgtta ctgctgttcg gtaagccagc cataaccctg
     2821 ctcctccagt tgtttgacca acgtgaaatc gccggatttc acaattcgtc cctgatatag
     2881 cacatggacg taatcaggct tgatgtagtc gagaatgcgt tggtagtgcg taacaatgat
     2941 gaatgagcgc ttgccatcac gcagggagtt cacgccatcg gcgaccactt ttaatgcgtc
     3001 aatatccagc ccggagtccg actcatcaag aatgcataac tccggttcca gcaccgccat
     3061 ttgcaaaata tcgttgcgct ttttctcgcc gccggaaaaa ccaacgttta ccgaacgggt
     3121 taataaatct tccggcatct tcaggagagc gattttctct tccatcaaat cctgaaaatc
     3181 aaagcggtcg agcgtttcct ggccgcgata gctgcgcact gcattaagtg ccgtttgcag
     3241 gaaaaactgg ttactgacgc ctggaatctc caccggatac tggaaggcca taaagatgcc
     3301 ttcgcctgca cgatcttccg gcgacagtgc aagcaaatct ttgcctttga actcaaccgt
     3361 gccgcccgtc acttcataat cttcgcgccc ggcaagcgtt gccgataagg tacttttgcc
     3421 cgaaccgttt ggccccataa tggcgtgaac ttcgccggga cgaacggcga ggcttaatcc
     3481 acgcaggata gctttatctt ccacgctgac gtgtaaatct ttaatactta acatgtttat
     3541 tccttatccg acgctgtgtt caagactgat cgcgaggagt ttttgtgctt caacggcaaa
     3601 ttccagcggc agttctgaga acacgtcttt gcagaaaccg ttaacaatca tcgagatggc
     3661 gtcttcttcg ctgatcccgc gttgcaggca gtaaaacagt tgatcttcac caatacgtga
     3721 tgtcgttgcc tcgtgctcca gttgtgcgct gttattgcga cattcgacat acgggaaggt
     3781 atgcgcccca caattagcgc caatcagcat tgagtcgcac tgagtgaaat tgcgcgcatt
     3841 ggttgccgtc ggcatgattt tcactaagcc gcgataactg ttctgactat gtccggcaga
     3901 gatccctttc gagataatgg tcgatttggt gtttttaccg atgtggatca tcttggtgcc
     3961 ggtatccgct tgctgatgac cgctggtcag cgccactgag taaaactcac caatggagtt
     4021 atcgccgcgc aaaatgcagc tgggatattt ccacgtaatc gcggacccgg tttctgattg
     4081 cgtccatgac attttgctgt tttcgccttc gcacaaagca cgcttggtga cgaagttgag
     4141 aataccgccg gtgttgttat cgccagggaa ccagttttgt accgtggaat atttcacctc
     4201 ggcgttttta tggatgatga cttccaccac tgccgcgtgt aactgatagc tgtcacgcac
     4261 cggagcggaa cagccttcaa tgtagctgac gtagctgtct tcgtcggcca ccagaatggt
     4321 gcgctcaaac tgcccggttt tttccgcgtt aatgcgaaaa taggtggaaa gttccatcgg
     4381 gcagcgcacg cctttaggca cataaataaa cgtaccgtca gaggctaccg ccgcattaag
     4441 cgcggcaaag aagttgtcat tccccggcac cacggtgccg agatatttac gcaccagttc
     4501 cgggtgatcg tggatcgcct caccaaagga acagaaaata attccctgct ccgccagttt
     4561 ttcgcgataa gtagtggcaa ccgaaacaga gtcgaaaatg gcatccaccg ccacctcttt
     4621 gccttcccgc acgggaacgc ccaactgctc aaacgccgcc tccacctctt tacttaaaaa
     4681 ggcgttcgcg ccagtttgct gcaccgcgcc aggttcagac gcgcaagtgt cgtcacaatt
     4741 accgcacgat ggtgctgagt agtagctgta atcctgataa ttcagcttgt cgtagtgcgc
     4801 tttcaaccag tgcggttctt ccatctccag ccatgcgcga taggcgttta gacgaaactc
     4861 cagcatccac tccggctcat tacgcttcgc cgaaattgcg cgcaccacct cttcgtttat
     4921 cccctttgcc agctcatcgg tggctaactg ggtgaagaat ccttctttat aattcagcgg
     4981 gccgccggtc caggttttga catcgtcagt tgcttcagta ttacgagaca tagtaccgcc
     5041 tataccccaa agctttcgcc acagccacat tcattctggg ctttagggtt gtgaaatttg
     5101 aatatctgat taagtccttc acgaacgaaa tcgacttccg tgccatcaat aaacggcatc
     5161 gcttgcagcg ggacaaacag cttcgcgccg tcgtgttcaa acagcagatc gtctttgtcc
     5221 ggctcgctaa cactgtcgag cacatagcca aagcccgcgc agcccgtttg cttcacgcct
     5281 aaacgcacgc cgaccatacc cggctgcttt gccaccagct cacggatgtg tatcgccgct
     5341 gcgggtgtca gcgttaagcc ttgccaggcg aaatcttgtg ggttaaaggt tcctgaatgc
     5401 atgtccatcg atttacctca cttcatcgct ttcagcgtat aacagcatgt tggtgataat
     5461 gattatcagt tcaacccagc aaacgcaggg gctttaccgt aaaacatgct ttttgcctgc
     5521 ttttaataag catagaccct gatgtgtggg ttaacaggca cgctaagaat taggtatctc
     5581 attgttagat aatggttatt atctaaggag cattaaggct gtgatgaaaa agaaaaagtt
     5641 gtattaaaat gactatttaa gagataggta aaaaagtgca gcgttcagaa ataagaaaac
     5701 ccttaagtct gtgcgacaca ggcttaaggg tttctacccc atccggcgct tatctccggc
     5761 actctcagtg gcttagctct tgaaggggcg ataagaataa tctcataaag ctaacccgcc
     5821 gttttaacac aaactgcgat tagtattatt tttgaacaat atcaggcggt agataagcag
     5881 tattaagaag gtcatcgaac ctggacggag gttaatccag gtcgatttgg cgaacttgcg
     5941 gcattaagtc aggatcaatg cacgcccaga cgccaggcaa agtagatttc ttcttttaat
     6001 tcagcagaag agagagtaag caggtcagca aattcaagtt ctagttgttt cagacgtttg
     6061 agatattggg caggtgaaag attgctctgg tcacggcgta aaaattcaat ggccagctgg
     6121 gtgggatcaa gttgagtaga catagcatcc tcgcttttag acaagacgtg tacagtatac
     6181 caccgtttac tgtgcagata atgaccaaaa gcaatatgcg tcacactttt ctggtgacaa
     6241 cgtcacaaaa tggcggtcgt caatcgtgac gaacagcaca aacgcccttt ctcatcgaag
     6301 atttcaatct gccagacctg gtgacgcgaa ccgagatgca acggtttaca tatgccgcgc
     6361 acccgccctt ctcgtgccga gcggacgtga ttagcattga tttccagacc aaccactttt
     6421 tgctcacctt cggtacataa ataaccggca acggaaccga tactttcggc cagtaccacg
     6481 gatgctcctc catgcagcaa cccgaaaggc tgctttgtcc gcgagtctac tggcattgtc
     6541 gcttcaaggg tgtcatcacc aatatgttca aagcgaatat ccaggaaccc caccatgttt
     6601 ccttcaccca tagcattcag tgcttccagg gtgattttcc gtttccatat catttaataa
     6661 tctccagtaa agcctgcaca ggatggcgta cccccgtgcc ttcaacccgt tttacctggc
     6721 tacggcagga atatccggtc gccagacagc ggttacgcgg cagtcgctgc atagcctgat
     6781 gccaggataa ctcatagatc ccgagcgaat tttcatggtt tttcgcttca tgtccgtaag
     6841 tccctgccat gccgcagcaa cccacgctga cattttccag tttcgcgcca aaacgggcaa
     6901 atatcgcggc ccattgtgct ggcgcacccg gcaaggcggt aacttcggta cagtgaccaa
     6961 agaaatacca tgattcaccg ctgactgtag ccacaggctg tgactcaagt gcgcttgcca
     7021 gccattcatt cgccagtaag acgttaaact cgccacgctc ctcgcccagc gccagtttat
     7081 attcatcgcg ataacaaagt accagcgccg gatcgacgcc caccattggc atacccagct
     7141 tcgccatacg gttgaggaaa tccgccgtct ttttcgccgt cttcgcaaaa cgattaagaa
     7201 aacctttaat atgctgggct ttgccatttg gcgaaaatgg cagtaacaca ggctggaaac
     7261 ctaatttttc gaccagacgg acaaaatccg ccaccacttg cgcatcgtaa tagctggtaa
     7321 aggggtcctg caccaccaac actgtgcgcg ctttctgctc tgcattgaga gattcaagct
     7381 gttccagcgt catgtttgcc gagcgatgcc ccaccatttg ttgttgtagc gaggggaccg
     7441 acagcagcgg caaatcaacc atgccgatat gtttttccga gagtttgcgc accagcggct
     7501 ggttaatgaa gaagttaaag gttttcggcg cgcgtgccat cagcggcgcg tagctctcga
     7561 ccgtagcgac gaggtggtcg cgcagcgggc gtaaataacg ggtgtgatag agctgcagaa
     7621 aacgagagcg aaactccggc acatcaattt tgatggggca cagggtcgaa cacgctttac
     7681 aggccagaca gcccgacatc gcctctttga cttcgtgtga gaagtcatat tcgcctttat
     7741 tcgcatgcca gctattgcgc gtgcgggcaa ttaacgtccg caaactgacg ccggattcag
     7801 gcagttcttg ttccagtttg agtggatcaa cgccgcgatc cgccaacaaa cgcagccatt
     7861 cacgcaccag cgttgcgcgc cctttcggtg aatgaatccg gttctgggtg atcttcatcg
     7921 acggacacat cggactacgg gcatcaaagt tgaagcataa accgttgccg ttacactcca
     7981 tcgcaccgcg ccactgctgg cgtaccgcaa tggggatctg ccgatcgaat gtaccgcgct
     8041 tcaccgcgtc cactttcatc atcggcgcat cgagaccttc tggcgggcaa atcttccctg
     8101 ggttgagtcg gttatgcggg tcaaatgccg ctttcacttt gcgcagttct gcaaaaagtt
     8161 cctcaccgaa aaacgccggg ctgtattcag cgcgaaaacc tttgccgtgc tcgccccaca
     8221 acaaaccacc gtatttcgca gtcagcgcca ccacgtcatc agagatttgc ttcatcaaaa
     8281 tctcttgttg aggatcgcac atatccagct ctggacggac gtgcaagaca cctgcgtcga
     8341 cgtgaccgaa cataccgtag cttaagccgt ggctgtcgag cagcgcgcga aattcagcaa
     8401 tataatccgc caggtgttcc ggcggtacgc aggtatcctc agcaaacgga attggcttag
     8461 cggcaccttt ggcattgcca agcagaccaa cggctttttt gcgcattgca tagatacgtt
     8521 caacccccgc cagctcacgg cacacctgcc agccgatgac acctgcttga tgactggcga
     8581 tcagctcatc caaccgcgca cagagtgcat ttacccgctc atcaatcagc gcctcatcat
     8641 caccagcaaa ttccacaatg ttcagcccga gcatctcttg gtcaggcaca tcggtaatca
     8701 actcgctgac ggaatgccag acaatatctt cccgcgccag attcagcact tttgagtcca
     8761 ccgtctctac cgaaagcgcc cgcgcctcaa ccataaacgg cgcgttacgc agcgcagagt
     8821 caaaagagtc atatttgacg ttcaccagac ggcgcacttt aggcaagcgc gtaatatcca
     8881 gccgcgcttc ggtaataaag gccagcgtcc cttctgaacc cgtcagaatg cgcgtcaggt
     8941 cgaactcggt catctcatcg ttaaagacat gacgcagatc gtaaccggta agaaagcggt
     9001 taagtttggg gaagttgtcg ataattaact ggcgttgctg acggcaacgt tgataaaccg
     9061 tgttataaat tcgcccgatt gtggtattgg atttacccag cgtttccgcc aattcgacgg
     9121 gtaaaggttg cgtatcgaga acatcgcccc ccaacaacac cgcgcgtacg ccaagtacgt
     9181 gatctgacgt tttgccatag accagcgatc cctgaccgga tgcatcggta ttgatcatcc
     9241 caccgagcgt tgcccggttg ctggtcgaaa gttccggcgc aaaaaagtag ccgaacggtt
     9301 tcaggtactg attgagttga tcttttatca ccccggcctc aacgcgcacc cagccctctt
     9361 cagggttaat ttcgatgatg cggttcatat ggcgggacat atcaacaata atcccctggt
     9421 tgagcgcctg accgttagtg ccggtgccgc cgccgcgggg ggtaaagatc agcgatgaat
     9481 agcgttcctg cgcggcaaga cgggcgatca gcgccacatc tgcggttgaa cgcggaaata
     9541 ccaccgcatc ggggagaagt tggtaaatac tgttgtcggt cgacattgtc agacgatcgg
     9601 cataacttgt cgccgtatcg ccggtaaaac cttgttgctc cagctcttgc aaaaaattaa
     9661 gcaccagttg aacgacgccg ggtgcctgag aaatctgtgg aatcattata ttgccccttt
     9721 cctgcggtct gtgatgtagg tcgatacact attctttcag gcggctgcaa tagcgcactg
     9781 aaaggtgatg tttatttact ctatggattt cgagttgcag gaaggcggca agcgagtgaa
     9841 ctccaggagc ttacaatagt aagtgactgg ggtgaacgaa cgtagccgca gcacatgcaa
     9901 cttgaaatac gacgagtaaa tcgtttgcgt gttgcctgag ttgttgtacc acattttttt
     9961 ctaacacgcc catcagaatt aagggcagaa tcggcctgtt aaaaaccgct gaaattgctc
    10021 atcattatgc aggtgagttt cgcgtgttca cgtcgcgtcg acgatttgac gcacaaaaaa
    10081 ggtgaaaagt agttatggta aatgttcgtc agcccaggga tgtcgcacaa attctgcttt
    10141 cggtgctgtt tttagccatc atgattgtgg catgtctgtg gattgttcaa ccctttattc
    10201 tcggctttgc atgggccggt acggtggtta tcgccacctg gccggtattg ttacgtttgc
    10261 aaaagatcat gtttggccgc cgctctctcg ccgttctggt gatgacgctg ttattagtga
    10321 tggtgtttat catccctatt gctttgctgg ttaacagtat cgtcgacggc agcggcccgc
    10381 taattaaagc catttccagc ggcgacatga cgttacccga tctggcgtgg cttaatacca
    10441 ttccggtgat tggcgcgaag ctgtatgcag gctggcacaa cttgctggat atggggggga
    10501 cggcgatcat ggcgaaagtc cgcccttata ttggcaccac caccacctgg ttcgttgggc
    10561 aggcggcgca tatcgggcgc tttatggtgc attgtgcgct gatgcttctc ttcagtgccc
    10621 tgctgtactg gcgcggtgaa caggtggcac aaggcattcg ccattttgca acccgtctgg
    10681 caggcgttcg cggagatgcc gccgtactgc tggcggcaca ggctatccgc gcggtggcgc
    10741 tgggtgtggt ggtgacggcg ttagtacagg cagtgcttgg cggtatcggc ctcgccgtat
    10801 ccggcgtacc ttatgcaacg ttgctaacgg tgttaatgat cctctcctgc cttgtccagc
    10861 ttggcccgtt gccggtactg attccggcga ttatctggct ctactggact ggcgatacca
    10921 cctggggaac ggtattgtta gtgtggagcg gtgtggttgg cacgctggat aacgtcatcc
    10981 gcccaatgtt aattcgcatg ggtgccgatt taccgctgat cctgatcctc tctggtgtta
    11041 ttggtggttt gattgctttc gggatgatcg gtctgtttat tggtccggtt ctgttagccg
    11101 tttcctggcg tctgtttgcc gcgtgggtgg aagaagtccc gccgccgacg gaccaaccgg
    11161 aagaaattct cgaagaactt ggcgaaatcg agaaaccgaa taagtaattt ctcatcaggc
    11221 ggctctgccg cctgattgtt aacccccgct aattgatatt tgcttgctct tccccatatt
    11281 ttcctgctta ccgccattca gctggtagta cctgtcgcaa attctttaca gtttttaaac
    11341 taatgagacg aatctgatcg acgcaaaaag tccgtatgcc tactattagc tcacggttat
    11401 aaatcaacat attgatttat aagcatggaa atcccctgag tgaaacaacg aattgctgtg
    11461 tgtagtcttt gcccatctcc cacgatgggc ttttttttaa catttttcca cttcgctacc
    11521 tcgcccctca ctctttccaa taatgttgca caagatactg tgactctctt caagccgtca
    11581 gcaggctgat aatgttattt atattttatt gtttatttca tgcagccatt acacgatgaa
    11641 cgcttatgaa ctccaggcac tccgacatat ttttgccatg actattgatg aatgcgcacc
    11701 tggattgccc agaccggtga cagtgaaagc tggcgtcagt gggaaaatgg caagtgtgcc
    11761 attcctgatc gtgtagtcga gcaactgttg gctatgcgtc agcaaagaaa aaaacatctt
    11821 catgccatca tagagaaaat aaataaccgc atcggcaaca acaccatgcg ttttttcccc
    11881 gacttaaccg catttcaacg agtctaccct gatggcaatt ttattgactg gaagatctat
    11941 caatcggtag ccgctgaagg taatgctgtt gaaaagcaaa ttccccagcc agttggtgga
    12001 cgccagcgta gctaatgaat taccgcaaat gaacagcaaa ccgttagtct ggctggaagg
    12061 tgtttcatcg gtactgttcg gtgtagccgc attctcgacc ttttatgtga ttgtggtgtg
    12121 gatgcccaaa tatgcgatgg cttttgctgg tatgtcagaa gctgaggcat taaaaaccat
    12181 ctcttattac agtatgggct cgttggtctg tgtctttatt tttgccgcac tactgaaaaa
    12241 aatggtccgg cccatctggg ctaatgtatt taactctgca ctggcaacaa taacagcagc
    12301 cattatctat ctgtaccctt ctccactggt gtgtaatgcc ggcgcctttg ttatcggttt
    12361 ctcagcagct ggcggcattt tacagctcgg tgtttcggtc atgtcagagt ttttcccaaa
    12421 aagcaaagcc aaagtcacca gtatttatat gatgatgggt ggactggcta actttgttat
    12481 tccgctgatt accggttatc tgtcgaacat cggccttcaa tatatcattg ttctcgattt
    12541 tactttcgcg ctgctggccc tgattaccgc aattattgtt tttatccgct attaccgcgt
    12601 ttttattatt cctgagaatg atgtgcggtt tggcgagcgt aaatttagca cccggttaaa
    12661 cacaattaag catagaggtt aaaggagtta attatggatg ttaccgcaaa atacgaattg
    12721 attgggctga tggcctatcc tatccgccac agtttatcgc ctgaaatgca gaataaagcc
    12781 ttagaaaaag cgggattgcc atttacctat atggcctttg aagtggataa cgatagtttt
    12841 cccggagcaa ttgaaggatt aaaagctcta aaaatgcgcg gaactggtgt atcaatgccg
    12901 aacaaacaac tggcttgtta atatgttgat gaattaacgc cagcagccaa actggtgggt
    12961 gccatcaaca ccatcgttaa tgatgatggc tacctgcgcg gctataacac cgacggcacg
    13021 ggccatattc gcgccattaa agagagcggt tttgatatca aaggcaaaac aatggtgctg
    13081 ttaggggccg gaggtgcctc aacggctatt ggcgcgcagg gggcaattga aggtttaaaa
    13141 gaaattaaac tctttaaccg tcgggatgag ttcttcgata aagccctcgc cttcgcgcag
    13201 cgggttaacg aaaacaccga ttgtgtcgtc acggttaccg atctcgccga tcagcaagcc
    13261 tttgctgaag ccctggcttc cgccgacatt ttaaccaatg gcacaaaagt gggtatgaaa
    13321 ccccttgaga atgaatcatt agttaatgat atcagtctgt tacatccggg acttctggtc
    13381 actgaatgcg tgtataaccc gcatatgacg aagttattgc agcaggcgca acaagctggt
    13441 tgcaaaacga ttgatggata cggcatgttg ttgtggcaag gggctgaaca gttcacgtta
    13501 tggactggca aagatttccc tctggaatat gttaaacagg tcatggggtt cggtgcctga
    13561 caggctgacc gcgtgcagaa agggtaaaaa aatgaaaacc gtaactgtaa aagatctcgt
    13621 cattggtacg ggcgcaccta aaatcatcgt ctcgctgatg gcgaaagata tcgcccgcgt
    13681 gaaatccgaa gctctcgcct atcgtgaagc ggactttgat attctggaat ggcgtgtgga
    13741 ccactttgcc gacctctcca atgtggagtc tgtcatggcg gcggcaaaaa ttctccgcga
    13801 aaccatgcca gaaaaaccgc tgctgtttac cttccgcagt gccaaagaag gcggcgagca
    13861 ggcgatttcc accgaggctt atattgctct caatcgtgca gccatcgaca gcggcctggt
    13921 tgatatgata gatctggagt tatttaccgg cgatgatcag gttaaagaaa ccgtcgcctg
    13981 cgcccacgcg catgatgtga aagttgtcat gtccaaccat gacttccata aaacgccgga
    14041 agccgaagaa atcattgccc gtctgcgcaa aatgcagtcc ttcgacgccg atattcctaa
    14101 gattgcgctg atgccgcaaa gtaccagcga tgtgctgacg ttgcttgccg cgaccctgga
    14161 gatgcaggag cagtatgccg atcgtccaat catcacaatg tcaatggcaa aaactggcgt
    14221 aatttctcgt ctggctggtg aagtatttgg ctcggcggca acttttggtg cggtaaaaaa
    14281 agcctctgcg ccagggcaaa tctcggtaaa tgatttacgc acagtattaa ctattttaca
    14341 tcaggcataa gcaataatat ttcggcggga atatcctccc ccgccgaact aaaaaaaata
    14401 tattcaaccg tatttaataa aaatatttcg tgagtctctg tgcgctaaat atccatctgg
    14461 cgtagggaaa atcacacctg aatcagaaat taacaatgaa acctgtaaaa ccacctcgta
    14521 ttaatggacg agtgccggtc ctgtcggcac aggaagcggt gaattatatt cccgacgaag
    14581 caacactttg tgtgttaggc gctggcggcg gtattctgga agccacgacg ttaattactg
    14641 ctcttgctga taaatataaa cagactcaaa cgccacgtaa tttatcgatt attagtccaa
    14701 cagggcttgg cgatcgcgcc gaccgtggta ttagtccgct ggcgcaagaa ggtctggtga
    14761 aatgggcatt atgtggtcac tggggacaat cgccgcgtat ttctgaactc gcagaacaaa
    14821 ataaaattat tgcttataac tatccacaag gtgtacttac acaaacctta cgcgccgccg
    14881 cagcccacca gcctggtatt attagtgata ttggcatcgg gacatttgtc gatccacgcc
    14941 agcaaggcgg caaactgaat gaagtcacga aagaagacct gattaaactg gtcgagtttg
    15001 ataacaaaga atatctctat tacaaagcga ttgcgccaga tatcgccttc attcgcgcta
    15061 ccacctgcga cagtgaaggc tacgccactt ttgaagatga ggtgatgtat ctcgacgcat
    15121 tggttattgc ccaggcggtg cacaataacg gcggtattgt gatgatgcag gtgcagaaaa
    15181 tggttaagaa agccacgctg catcctaaat ctgtccgtat tccgggttat ctggtggata
    15241 ttgtggtggt cgatccggat caaagccaac tgtatggcgg tgcgccggtt aaccgcttta
    15301 tttctggtga cttcaccctt gatgacagca ccaaacttag cctgccccta aaccaacgta
    15361 aattagttgc gaggcgtgca ttattcgaaa tgcgtaaagg cgcggtgggg aatgtcggcg
    15421 tcggtattgc tgacggtatt ggcctggtcg cccgggaaga aggttgtgct gatgacttta
    15481 ttctgacggt agaaacaggt ccgattggcg gaattacttc acaggggatc gcctttggcg
    15541 cgaacgtgaa tacccgtgcc attctggata tgacgtccca gtttaatttt tatcacggtg
    15601 gtggtctgga tgtttgttat ttgagttttg ctgaagtcga ccagcacggt aacgtcggcg
    15661 tgcataaatt caatggcaaa atcatgggta ccggtggatt tattgatatc agtgccacct
    15721 cgaagaaaat tattttctgc ggcacgttaa ccgcgggcag tttaaaaaca gaaattgccg
    15781 atggcaaatt aaatatcgtc caggaaggac gggtgaagaa atttattcag gaactaccgg
    15841 aaattacttt tagcggaaaa atcgcgctcg aacgggaact ggatgttcgt tacatcaccg
    15901 aacgtgctgt attcacgctg aaagaagacg gcctgcattt aatcgaaatc gcccctggcg
    15961 tcgatttaca aaaagatatt ctcgacaaaa tggatttcac cccagtgatt tcgccagaac
    16021 tcaaactgat ggacgaaaga ttatttatcg atgcggcgat gggttttgtc ctgcctgaag
    16081 cggctcatta acaggagtat cacgatggat ttttctttaa ctgaagaaca agaactgctg
    16141 ctggccagta ttcgcgaact gattacgact aactttgcgg aagagtattt ccgcacctgc
    16201 gatcaaaacg ggacatatcc gcgtgagttt atgcgggcgc tggcggataa cggcatttct
    16261 atgcttggcg tgccggaaga gttcggtggc atccctgcgg attacgtcac ccagatgctg
    16321 gcgctgatgg aagtgtcaaa atgcggtgct ccggcatttt tgattaccaa cggtcaatgt
    16381 attcacagta tgcgccgttt cggttctgca gagcagctac gtaaaacggc agagagcacc
    16441 ctggaaacgg gtgatcccgc ctatgccctg gcgttgacgg agccaggcgc aggctcagac
    16501 aacaacagtg ccactaccac ttacacgcgt aaaaacggca aggtttacat caacggacag
    16561 aaaaccttta ttaccggtgc gaaagagtac ccgtatatgc tggtgttggc gcgcgatccg
    16621 caaccgaaag atcccaaaaa agccttcacc ctgtggtggg tcgactccag taagcccggc
    16681 attaagatta acccgctgca taaaatcggc tggcatatgc tcagcacctg cgaagtctat
    16741 ctcgacaacg tggaggttga agagagcgac atggtgggcg aagaaggaat gggtttcctc
    16801 aatgtgatgt acaactttga gatggagcgc ctgatcaacg ccgcgcgcag caccggcttt
    16861 gccgaatgcg cctttgaaga tgccgcccgc tatgccaacc aacgtatcgc ttttggtaag
    16921 cccattggtc ataaccagat gatccaggaa aaactggcgc tgatggcgat taagatcgac
    16981 aacatgcgca acatggtgct gaaagtggca tggcaagccg atcagcatca gtcactgcgc
    17041 accagcgcgg cgctggcaaa actgtattgc gcacgtaccg caatggaagt cattgatgat
    17101 gcgattcaaa tcatgggcgg tctgggctat accgatgaag cgcgcgtctc ccgcttctgg
    17161 cgtgatgtcc gttgtgaacg tatcggcggc ggtacagacg aaattatgat ttacgtagca
    17221 ggtcggcaga tcctgaaaga ctatcagaac aaataatctg caggcggcgc agcttcttaa
    17281 caaactgcgc cgccagattt atccaacaag acttaccggt tgaggaaatt cagataggat
    17341 ttcttgttcc gcaaaacagt ttttaaattg tctacggtaa tcgctgggcg aacaaccgac
    17401 atggcgcaaa aacagtttgg caaagtgatc gacattttca taacccacac gccaggagat
    17461 ctctgcctgt gataattcag tattagtgag tgaccatttt gcttccgtca tacgccgttg
    17521 aataacatag ttaatagggg aaatacgata ctctttggta aattcatggc aaatataact
    17581 gacgctggca cgaaattttt tcgataactg ttctaaagtg attttttcgc gataattatt
    17641 actcagatag aaaagaatat cttttatcag aacatctttt ttaatatacc cctgctccga
    17701 acgataagca tttttaaaat tttcgtagta aagaatggct aacgtatagg caaatgcatc
    17761 ccataacgaa gatgtttggc tatttttact ttgcggcaaa atcacactta gctcattaaa
    17821 taaggttttt atgacttctt ttccctgccc cgcggcaatt accggacaag aatgcggttg
    17881 cagtagctga ttttcctcag ccccctgaaa ctgaaagccg tacagcgcac aggtacacgt
    17941 cgttgccgga tcgttaacgt cagaggccac cgcatgcagc ctgccgcgtt ctatcaccac
    18001 aatgtcatcc gcatgtgcga catataacga agaatcgatg gtaaaccttg caaccccttt
    18061 cttgacgtaa atcaactcgg tttcattgtc atgaacgtga tgtccggact cccattttgg
    18121 atcatcgcta aatgcaaaac gtgaaagccg tggcgtttta ccggcgacaa acagcgtttc
    18181 actggcgtta tcaaaacagc gttgatacat gacaacctcc ctattccatg agcaagcaaa
    18241 aacaatatat gccggataaa atacggcgtg tattgcggtt atacaaccgc gtttagcgag
    18301 agctaaacaa gattatttac actgaatgca agattgtacg gtcatggaat aactatattg
    18361 ccgcaaattg cacttttgtt ttagcgatcg catttttttg caagattgtt ggcaaggaaa
    18421 acagcttgct ccgtcgaaaa cccccaccgc tatcgcacac tattttcagg ccatttttac
    18481 cttccatcgg agatggttcc gtatgcgact cacaggagaa atcatgaaaa taataacctg
    18541 ctttaagctg gtgcctgaag aacaggacat tgttgtcact ccagaataca ccctgaattt
    18601 cgacaatgcc gacgccaaaa tcagccagtt cgatctcaat gccattgaag ctgcaagcca
    18661 gctcgcaaca gatgacgatg agatagccgc gctgaccgtt ggcggctcat tgttgcagaa
    18721 ctcgaaagtg cgcagtgcgc aaagatgtgc tatcccgcgg gccgcacagc ctgtatttgg
    18781 tgcaggatgc gcaacttgaa catgcactgc ctctcgatac cgcaaaagcg ctggcggcag
    18841 caattgaaaa gatcggcttc gatttactga tctttggtga aggttccggc gacctttatg
    18901 cccagcaggt tggcttgctg gtcggagaaa ttctgcaact tccggtgatt aatgcagtga
    18961 gtgctattca gcgtcagggt aatacactgg tgattgaacg cacgcttgaa gatgatgttg
    19021 aagttattga actctctgtt ccagccgtgc tctgcgtcac ctccgatatt aacgtgccac
    19081 gcattccttc gatgaaagcc attctcggcg cgggtaaaaa accggtaaat cagtggcagg
    19141 caagtgatat tgactggagc cagagcgcgc cacttgctga actggtcggc attcgcgtac
    19201 cgccgcaaac agaacgtaag cacatcatta tcgataacga ttcgccggag gccattgccg
    19261 agctggcgga acatctgaag aaagccctga actgaagcct acggagaaga aacgatgagt
    19321 caattaaaca gcgtctgggt ctttagcgat aatcctgaac gttatgctga actgtttggc
    19381 ggcgctcagc aatggggcca acaggtgtat gccattgtac aaaataccga ccaggcgcag
    19441 gcagttatgc cttatggtcc aaaatgtatt tatgttcttg agcaaaacga cgcgctgcaa
    19501 cgcactgaaa attacgccga aagcattgct gccccgctga aagataaaca ccccgctatg
    19561 ttgctgttgg tcgcgacgaa acgtggtaaa gctctggcag cacggttaag tgtgcaactg
    19621 aatgcggcgc tggtgaacga tgccacggcg gtggatattg tcgatggtca catttgcgcc
    19681 gaacaccgga tgtatggcgg gttagcgttc gctcaggaaa agatcaacag cccgctggcg
    19741 atcattaccc ttgcacccgg tgttcaggaa ccgtgcacca gtgatacctc tcatcagtgc
    19801 ccgacagaaa cggtacctta tgttgctccg cgtcatgaaa ttctctgtcg cgaacgccgt
    19861 gcgaaagccg caagcagcgt ggacctgagc aaagcaaaac gtgtggttgg cgtcggtcgt
    19921 ggtctggcgg cgcaggatga cctgaaaatg gtccacgaac tggcggcggt gctgaatgct
    19981 gaagtcggct gttcacgtcc aattgccgaa ggcgagaact ggatggagcg tgaacgttat
    20041 atcggtgtct ccggcgtgtt gctgaaatcc gatctctacc tgacgctggg gatctccggg
    20101 cagatccagc atatggttgg cggcaacggc gcaaaagtga ttgtcgccat caataaagat
    20161 aaaaatgcgc caatcttcaa ttatgccgac tacggtctgg tgggcgatat ctacaaagtc
    20221 gtccctgccc tgattagcca gttgagccgc taattcccct ttcaaccaca tctcccgctg
    20281 taaacgcagc gggaagggag cataacgcat gtcggatgac aaatttgatg ccattgtggt
    20341 cggtgcggac gttgctagta gcgttgccgc actggtcatg gcgcgagccg gactggatgt
    20401 cctggtgata gaacgcggcg acagtgccgg atgtaaaaac atgaccggcg ggcgtcttta
    20461 tgcccacaca cttgaagcaa tcattccagg ctttgcagta tcagcgccgg tagaacgcaa
    20521 ggtcacacgc gagaaaatct ccttcttaac cgaagaaagc gccgttaccc tcgattttca
    20581 ccgcgagcaa ccagatgttc cgcaacacgc atcttatacc gtattgcgta atcgtctgga
    20641 cccgtggttg atggaacaag ccgagcaggc gggcgcacag tttatcccgg gcgttcgcgt
    20701 cgatgcgttg gttcgtgaag gaaacaaggt cactggcgtg caggccgggg atgatattct
    20761 ccaagcgaat gtggtggttc tagctgatgg cgtaaactcg atgcttggcc gctcgctggg
    20821 aatggttccc gcttccgatc cgcatcatta cgctgttggt gttaaagagg ttattggcct
    20881 cacaccagaa cagatcaacg atcgctttaa tattacgggc gaggaaggtg ccgcctggct
    20941 gtttgccggc tccccttctg acggcctgat gggcggggga tttctctata ccaacaagga
    21001 ttccatatcc ttggggctgg tttgtggatt gggtgatatc gcccatgcgc aaaaaagcgt
    21061 gccgcaaatg ctggaagatt ttaaacaaca ccccgccatt cgcccactaa ttagcggcgg
    21121 caaactgctt gaatattccg cgcatatggt gccagaaggc ggtctggcaa tggtgccgca
    21181 gatggttaac gatggcgtga tgatcgttgg tgacgccgca ggcttctgcc tgaatttggg
    21241 ttttacagtt cgtggcatgg atttagccat tgcatcggct caggctgccg ccacaacggt
    21301 gatcgccgcc aaagaacgcg aggatttctc cgccagcagt ctggcgcaat acaaacgtga
    21361 gctggaacaa agctgcgtca tgcgcgatat gcagcatttc cgcaagatcc cggcgctgat
    21421 ggaaaacccg cgcctgttta gccaatactc acgaatggtc gccgacatca tgaacgagat
    21481 gttcaccatt gacggcaaac caaaccagcc ggtacgcaaa atgatcatgg gacacgcgaa
    21541 gaaaattggg ctgatcaact tgctgaaaga tggcattaag ggagcaaccg cgctatgagc
    21601 cagaacgcta cggttaacgt cgacatcaaa ttaggcgtca ataaattcca tgttgatgag
    21661 ggccacccgc atatcattct ggcggcaaat cccgatatca atgaattccg taaattaatg
    21721 aaagcttgcc ctgccggact ttataagcag gatgacgccg gcaacattca tttcgattcc
    21781 gccggttgtc tggagtgcgg cacctgtcgg gtgctgtgcg gcaacactat tctcgaacag
    21841 tggcaatatc ccgcaggcac cttcggtatt gattttcgct acggctaatc atgcatccca
    21901 caggcccgca tctcgggcct gatgttctgt ttcgagagtc caaaatgaaa gtgacattaa
    21961 cgttcaacga acaacgtcgt gcggcgtatc gtcagcaagg gttatggggc gatgcttcac
    22021 tggccgatta ctggcagcag accgctcgtg cgatgccaga caaaattgcc gtggtcgaca
    22081 atcatggtgc aacgtacacc tatagcgcgc tcgatcacgc cgcgagctgt ctggcaaact
    22141 ggatgttagc gaagggtatt gaatcaggcg atcgcatcgc atttcaactg cctggctggt
    22201 gtgaatttac cgttatctat cttgcctgcc tgaaaatcgg tgcggtttcc gtgccgctgt
    22261 tgccttcctg gcgggaagca gaactggtgt cggtgctcaa taagtgtcag gcaaaaatgt
    22321 tctttgcacc gacgttgttt aaacaaacac gtccggtaga tttaatcctg ccgctgcaaa
    22381 atcagcttcc acaactacaa caaattgtcg gcgtggacaa actggctccc gccacctctt
    22441 ccctctcatt aagtccgatt atcgccgaca atacctcact gaccacggcg ataacgaccc
    22501 acggcgatga attagctgcg gtgctgttta cctccggaac cgagggtctg ccaaagggcg
    22561 tgatgctaac gcataacaat attctcgcca gtgagcgggc ttattgcgcg cgactgaatc
    22621 tgacctggca ggatgtcttt atgatgcctg cgccacttgg tcacgcaacg ggctttctgc
    22681 atggcgtaac ggcaccattc ttaattggcg ctcgcagcgt gttgttagat attttcactc
    22741 ctgatgcgtg tctcgcgctg cttgagcagc agcgttgcac ctgtatgcta ggcgcaacgc
    22801 cgtttgtcta tgatcttttg aatgtactag agaaacaacc cgcggacctt tcagcgctgc
    22861 gtttctttct ttgcggcggt accacaatcc ccaaaaaagt ggcacgtgaa tgccagcagc
    22921 gcggcattaa attattaagt gtttatggtt ccacagaaag ttcgccgcat gcggtggtga
    22981 atctcgatga tcctttgtcg cgctttatgc acaccgatgg ttacgctgcc gcaggtgtag
    23041 agattaaagt ggtcgatgac gcacgcaaga ccttaccgcc aggttgcgaa ggtgaagaag
    23101 cctcgcgtgg ccccaatgtg tttatggggt attttgatga acctgaatta accgcccgtg
    23161 cgctggacga agaaggctgg tattgcagcg gcgatctctg ccgtatggat gaggctggct
    23221 atataaaaat taccggacgc aaaaaagata ttattgtccg cggcggcgaa aatattagca
    23281 gccgtgaagt ggaagatatt ttattgcagc atcctaaaat tcacgatgcc tgtgtggttg
    23341 caatgc
//
LOCUS       DAJTNN010000051        23331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTNN010000051 DAJTNN010000000
VERSION     DAJTNN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.051"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(299..613)
                     /gene="pspE"
                     /locus_tag="OO150_001765"
     CDS             complement(299..613)
                     /gene="pspE"
                     /locus_tag="OO150_001765"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5515941.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(688..909)
                     /gene="pspD"
                     /locus_tag="OO150_001766"
     CDS             complement(688..909)
                     /gene="pspD"
                     /locus_tag="OO150_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5515942.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(918..1277)
                     /gene="pspC"
                     /locus_tag="OO150_001767"
     CDS             complement(918..1277)
                     /gene="pspC"
                     /locus_tag="OO150_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5515943.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1277..1501)
                     /gene="pspB"
                     /locus_tag="OO150_001768"
     CDS             complement(1277..1501)
                     /gene="pspB"
                     /locus_tag="OO150_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5515944.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1555..2223)
                     /gene="pspA"
                     /locus_tag="OO150_001769"
     CDS             complement(1555..2223)
                     /gene="pspA"
                     /locus_tag="OO150_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5515945.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKESGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2390..3367
                     /gene="pspF"
                     /locus_tag="OO150_001770"
     CDS             2390..3367
                     /gene="pspF"
                     /locus_tag="OO150_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5515946.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3487..4752)
                     /gene="puuE"
                     /locus_tag="OO150_001771"
     CDS             complement(3487..4752)
                     /gene="puuE"
                     /locus_tag="OO150_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5515947.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4790..6059)
                     /gene="puuB"
                     /locus_tag="OO150_001772"
                     /pseudo
     CDS             complement(4790..6059)
                     /gene="puuB"
                     /locus_tag="OO150_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
     gene            complement(6061..7548)
                     /gene="puuC"
                     /locus_tag="OO150_001773"
     CDS             complement(6061..7548)
                     /gene="puuC"
                     /locus_tag="OO150_001773"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5515948.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FSDCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7823..8380)
                     /gene="puuR"
                     /locus_tag="OO150_001774"
     CDS             complement(7823..8380)
                     /gene="puuR"
                     /locus_tag="OO150_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5515949.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8407..9170)
                     /gene="puuD"
                     /locus_tag="OO150_001775"
                     /pseudo
     CDS             complement(8407..9170)
                     /gene="puuD"
                     /locus_tag="OO150_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            9382..10800
                     /gene="puuA"
                     /locus_tag="OO150_001776"
     CDS             9382..10800
                     /gene="puuA"
                     /locus_tag="OO150_001776"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5515950.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10949..11114
                     /locus_tag="OO150_001777"
                     /pseudo
     CDS             10949..11114
                     /locus_tag="OO150_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11104..12489
                     /gene="puuP"
                     /locus_tag="OO150_001778"
     CDS             11104..12489
                     /gene="puuP"
                     /locus_tag="OO150_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5515951.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12623..12868
                     /locus_tag="OO150_001779"
     CDS             12623..12868
                     /locus_tag="OO150_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5515952.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13181..14824
                     /gene="sapA"
                     /locus_tag="OO150_001780"
     CDS             13181..14824
                     /gene="sapA"
                     /locus_tag="OO150_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5515953.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLKFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14821..15786
                     /gene="sapB"
                     /locus_tag="OO150_001781"
     CDS             14821..15786
                     /gene="sapB"
                     /locus_tag="OO150_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5515954.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15773..16663
                     /gene="sapC"
                     /locus_tag="OO150_001782"
     CDS             15773..16663
                     /gene="sapC"
                     /locus_tag="OO150_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5515955.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMLAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16663..17655
                     /gene="sapD"
                     /locus_tag="OO150_001783"
     CDS             16663..17655
                     /gene="sapD"
                     /locus_tag="OO150_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5515956.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17657..18463
                     /gene="sapF"
                     /locus_tag="OO150_001784"
     CDS             17657..18463
                     /gene="sapF"
                     /locus_tag="OO150_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5515957.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18531..18884
                     /locus_tag="OO150_001785"
     CDS             18531..18884
                     /locus_tag="OO150_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5515958.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19252..20040
                     /gene="fabI"
                     /locus_tag="OO150_001786"
     CDS             19252..20040
                     /gene="fabI"
                     /locus_tag="OO150_001786"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5515959.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20185..21312
                     /locus_tag="OO150_001787"
     CDS             20185..21312
                     /locus_tag="OO150_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5515960.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTARDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     TAQFSPLLEHEQSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21380..23314
                     /gene="rnb"
                     /locus_tag="OO150_001788"
     CDS             21380..23314
                     /gene="rnb"
                     /locus_tag="OO150_001788"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5515961.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
BASE COUNT         5266 a         6176 c         6089 g         5800 t
ORIGIN      
        1 aagacgcgtt acaagtttct gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc
       61 caggtaatca gtaattttct gtttcatgtt gcctttcagt ggtagcgatc ccatgaaacg
      121 agagactact ccgcagaaaa taaactgtca attgagcaca agggttttag tcacgcagga
      181 aatcattttt taccagggaa aaagcgtatt tgtgagctga cgcaaataat ctatggggat
      241 gtaaataagg tgtgacagaa aaaaaacggc gcataagcgc cgctcatggt gaattctttt
      301 aacctttgac cttcggcatt gcgatgtctt tcaagccacc ggcgttctca acgtgggtat
      361 atcccatctc gctaaggatc tcttttgctt gccctgactg gcgtccggca ttgcaataca
      421 ctttcacggt gtcgttttta tccggaacgg cggtggcaat gcgctctttc acttccttca
      481 ggggaatatt gatggccccc tgaacgtgct cttgctgata ctgctctgga acacgaacat
      541 cgatccagtg ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga gcaagtaagc
      601 cttttttaaa catgtaagcg tccttctatg gatgaaaatt gccatccatt atacgcctga
      661 gatttaacaa tttttgcgaa actcccctca ccttttataa cgctgtgcca gtttattagc
      721 agcccgactt aacagcggtt ccagcgccac agccagcaac attttcagcg gtcggcgagc
      781 aactgatttt atcgcccagc ccgctacacc cgccgggcca tagcgcagtg cggtaagaag
      841 taccagcttg cctgctaatt tgaaaccagg ctttaccttt tgcccggcct gttgccagcg
      901 agtattcata actttcctca cagttgacgg aaacggctac gtaacgtgaa agtatcggaa
      961 gtgacataac gttccatctc gcgtaaacgc gtttcacttg ccgccagttc acggtcgact
     1021 tcatccagca attcgctgct ggagggtagc tgctcaccaa aggccatgtt gtccggcatt
     1081 ggatcaagcg caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag
     1141 aaaatcgaca gcaccaccag gatacgcacc agttttaccg gtacatcaaa atagttggca
     1201 atcccggcgc agacgccgcg gaccatgccc tgctgtggaa tacgccataa ttttttattg
     1261 agattaatgc ccgccattag cgatccctcc agttcggatg ttctgcatca agaatagatt
     1321 ccagcgcctg aatacgttcg cgcatccgtt ttgcttcatc agccagttgc gctaatcgct
     1381 gctgctcact ttgcgacaat tcactgcgac cagaacgatt gctgtaatgc agccataacc
     1441 agatcggtaa aacaaacagc acaaaaatgg ttaacggaat agccagaaat agcgcgctca
     1501 taagtactcc ttagacgatg agcggcggcg cgttcggacg ccgcctagat gttattattg
     1561 attgtcttgc ttcattttgg cttttaattg tgccagttgt tcgctgattg catcatcggc
     1621 tttcagttcg gcaaactgat cgtccagcga tttttgttta ccgaagctgt ggctttctgc
     1681 ttccgcttcc atctggtcaa tacgacgttc gaaagattcg aaacgagcca ttgcttcatc
     1741 cagtttgcca ctgtccagct gacgacgcac atcgcgcgac gagtttgccg cctgatggcg
     1801 taacatcaat gcctgctggc gagcgcgtgt ttcgctcaat ttgttttcca gctcaccgct
     1861 ctctttcttc atgcgtgcca gcgtatcgtc caccagcgtc acttcatgtt ccagactctt
     1921 aatcagatcg gtcagtttct gtttttcaat taacgccgca cgtgccaaat cctctctctc
     1981 tttcagcagc gccagttcgg ctttttcctg ccattcaact tcacgcgccg acgcttgttc
     2041 aatacggcga gtcagctgtt tcttttctgc caacgcacgc gccgaagtag aacgtacttc
     2101 aaccagtgta tcttccatct cctggatcat cagacgcacc agtttctgtg gatcttccgc
     2161 tttctctaac agagcgttga tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc
     2221 cataatgttg tcctcttgat ttctgcgttc aggattgtcc tgctgaactg ttaatacaat
     2281 ttgcgtgcca attttttatc tttttgattt ataaagatct gattgaagaa tcatcagcaa
     2341 catgccagga tgaattagcg aattacacta acaagtggcg aatttcatca tggcagaata
     2401 caaagataat ttacttggtg aggcgaacag ctttctcgaa gtgctggaac aggtttcgca
     2461 tctcgcaccg ctggacaaac cggtgctcat catcggcgaa cgcggcaccg gtaaagagct
     2521 gattgccagc cgcctgcatt atctctcctc ccgttggcaa gggccgttta tttcccttaa
     2581 ctgcgcggcg ttaaatgaaa atctgctgga ttccgaactg tttggtcacg aagcgggggc
     2641 gtttaccggt gcgcaaaaac gtcatccagg gagatttgaa cgtgccgacg gcggtacgct
     2701 atttcttgat gaactcgcta cggcaccgat gatggtgcag gagaaattat tgcgcgtgat
     2761 tgagtacggt gaactggagc gcgttggcgg cagccagcca ttgcaggtga atgtgcggtt
     2821 ggtatgcgcg acgaatgccg atctcccggc gacggtcaat gaaggcactt ttcgcgctga
     2881 cctgctcgac cgactggctt ttgatgttgt acaactgcca ccactgcgcg agcgcgaaag
     2941 cgacataatg ttgatggcag aacactttgc catccagatg tgtcgggaaa tcaagctgcc
     3001 tctgttcccg gggtttacgg agcgcgccag agaaacattg ctgaattatc gttggccggg
     3061 aaatattcgt gaattgaaaa acgtggtgga acgttcagtg tatcgccacg gcaccagcga
     3121 ttatccgctt gatgacatca ttattgatcc ctttaaacgg cgtccgcctg aagacgctat
     3181 cgccgtttca gaaaccacct cgcttccaac actgccgctg gatttacgtg agtttcagat
     3241 gcagcaggaa aaagagttgc tgcaactcag tttgcaacaa gggaaatata accagaagcg
     3301 cgcggctgaa ttactggggt taacttatca tcagttccgc gcgttgttga aaaagcacca
     3361 gatttagcga agttgagcaa tacaacttgc cggatgcggc gtgaatgctt tattcggcat
     3421 acataatcaa taagtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt
     3481 gggctattaa tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc
     3541 cggaatggtc agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag
     3601 gagcagcccc tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc
     3661 gcccgtttgc ggatcgttaa actctgccgc aatcatcgac cccaggccgc gtaccgcagc
     3721 aatggccgga acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg
     3781 attcgcgcgt tcgcagagtg attctttgtc gataatattg agcaccgcgt gcgcggcagc
     3841 caccgccagc gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat
     3901 aatattcgcg ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat
     3961 cgtcattaag tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc
     4021 aaagccgctt tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg
     4081 aatggcggca accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc
     4141 gaaaataatg gccgccacct gcttcgcttc aatgtccgat ttaaacaagc gttcgatggc
     4201 gtcgagggag tcctgtgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac
     4261 cgaaccaggg aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat
     4321 ggtcatatat gtacgaccgt ggaagccgcc gctaaacgca atcacgccag gtcgtccggt
     4381 atgggcgcga gcaattttca ccgcgttttc caccgcttcc gcgccggtag tgaagaatgc
     4441 ggttttagcc tgcccgctca ctggggcaag ggcgttaatt ttctccgcca gcgtgacgta
     4501 gctttcgtac ggcacaatct gatacgccgt gtgggtaaac tgttgcaatt gctgctccac
     4561 cgccgcgacc agatcagggt ggcgatgccc ggtattcagc accgcaatgc ctgcggcgaa
     4621 atcgatgtat tcgttgccct ctacgtcctt cagcgtagcg ttttctgccg actgggcgaa
     4681 gaagttacac atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc
     4741 attgttgctc atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca
     4801 gacgatcgcg caggctgtaa taagccgcgc ccatcgcggt aaatggcacg cgcagcgtgc
     4861 gcccgccggg gaacgggtaa tgcggcagat tggcgaaggc atcgaaacgc tcggcgtcac
     4921 cgcgcagcag ttcagcaatc aaacgcccgg cgaggtgggt acaggtgacg ccgtggccgc
     4981 tgtagccctg catgtaatag atgttggtat cgaggcgacc gaactgcggc atccgtgaaa
     5041 gggtcagcag gaagttgccc gtccagcgat agtcgatttt gacgcccttc agctgcggga
     5101 aggttttcag cagtttcggc acaacaagac gctcaacgtc atccgggtcg cgcgcgccgt
     5161 agaccacgcc gccgccgtac agcaaacggt tgtcggcggt aagacggtag taatcaagca
     5221 gataattaca gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg
     5281 acagccgttc ggtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gccagttccg
     5341 gctctacttt atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct
     5401 gacctttggc agtgcgcaca acggctggcg tggtgtgctg gatctgcgtc acggcagaaa
     5461 gttcatacac gcgcccgccg ttgaggcgga tggcgtccgc ttcgccaatg gcgaggttta
     5521 gcgggtgaat atgcccaccg ctgtgatcca gcagcgcacc ggtatagcga tcgctggcta
     5581 cttcacggcg aatggcgttg gcgtccagca attccagctg tttattgccg tagcgttccc
     5641 agttctcttt ctgctcttca agtgtggcga gctgtttatc attcatcgcc acaaacaggc
     5701 tgccggggcg gtagtcgcag tcaatctgat aacgtttgat acgttcgcgg atgatctcac
     5761 caccttcgaa catcatgctg ccgagcatag gggctgtgtc catgccgtag cttttttcga
     5821 tcacgtcgat gtcgcggttg taggagttca caagctgccc gccattgcgc ccgcttgcgc
     5881 caaagccgat gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca
     5941 gcgcggagga gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact
     6001 cattcagggt gtcgaatggc acgtatttat gcgtaataac tgctggtatg ttcggtcatt
     6061 tcaggcctcc aggcttatcc agatggtttt cagttcggtg aatttttcaa gggcatgcag
     6121 ggatttgtcg cgaccgttgc cgctctgctt atagccgcca aacggcacgg tcatatcgcc
     6181 gtcgttgtag ttattgacga agacggaacc ggctttcagg cggcggctca tacggtgcgc
     6241 gcgggagagg tcgcgcatcc ataccgccgc gccaaggccg tactggctgt cgttggcaag
     6301 ctgtagcgcc tgttcttctg atgtgaaacg cgtgaccacc agcaccggac cgaaaatctc
     6361 ttcgcggctt aaggacgcat tcgggtctac atccacaaag atggtcgggc cgatggcggc
     6421 agccagcccg gcgttacggc catccagcag cagttgccct ttgccttcgc cctcacgaat
     6481 aaagctatgg accgagtcgg cgtgggcgca gtcgattaaa gtgcccatgg tggttgcggg
     6541 gtcaagcgga tggcccggct gccagttttg cgcctgctgt tttaacaggg ctaagaattc
     6601 atcggcgatg ctctcttcca gcaacaggcg tgttccggcg atgcacacct gaccctggtt
     6661 gtagaagatg cctgctgcgg tggagctcgc cgctttttgc agatccgaac aatcggagaa
     6721 aacgatgttg gcgcttttgc cgcccgcttc cagccagacg cgtttcatgt tgctgtcgcc
     6781 cgcatctttc agcagctgtt tcccggtacg ggttgaaccg gtaaaggcaa tggcgtcgat
     6841 atcgttatga cgcgacagcg cctgccccgc ttcatgacca aaacccgtca ccacgttcaa
     6901 cacaccatcc ggcaagcctg cttctttcgc cagccccgcg agacgaatcg cactgagcgg
     6961 tgatttttca gacggtttta gaatcacgct gtttcccgcc gccagcgccg ggccgagttt
     7021 ccagcaagtc agcaacagcg ggaagttcca cggcacgatg gcggcaatca cgccgaccgg
     7081 ttcacgcacg atcatcgcca gctcatggct actggtggtc gccacttcgc catacacttt
     7141 gtcgatcgct tcggcgtacc agcgaatggc gcgcgccgcg ccgggaatat catcacgcag
     7201 actgtgacga atcggtttgc cggtgtcgag agtttccagc agtgccagct cttcggcgtg
     7261 ggcttccatt aaatcggcga gtttattcag taccgcttta cgtttagccg gagaagagag
     7321 tgaccagtcg ccgcgttcaa atacgccgcg tgctgcgctc accgcacggt cgatatcgac
     7381 gctcttgccg cgggcaattt tcgccagcgg tgcctgggtg actggatcaa cggtttcaaa
     7441 ggtttcattt tccgccgcag cagtatattc accgttaata aataagcggt tttcaatggc
     7501 gagacttaac gctttatcct gccagtaagc cagatgatga aaattcatta tgactcctgt
     7561 ttcacgtcta tcagatatat gcagtttgtg aacgtcaggg cgcagagatc cccgggccgt
     7621 atgcccgttt atttctggcg acattcttta tatacacttc atccttcaag ccgcctctgc
     7681 cttggctgcg ttcgctcccc cgaatcactt acctgagtaa gctcatcggg attcgctcac
     7741 ttgccgcctt gatgcaactt gaatgatttt gtgtatagat tgccgggcgc attgcgctta
     7801 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     7861 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     7921 gacccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     7981 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8041 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     8101 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     8161 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     8221 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     8281 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     8341 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     8401 tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga taaagccctc
     8461 gaacaatata cgcgaaagag cgtactcgct actgttccat tccgggtgcc actgtacgcc
     8521 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     8581 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     8641 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     8701 atgcgacggt gcatattgct gttccaccgg cagttcggat cttcccgatg ttccagcaat
     8761 tcaggctgtt cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac cagttcttgt
     8821 aaaccccggc agatggcgaa aatggggatg cgcctttcga gtgcggcatt aattatcgcc
     8881 atgctcagaa gatcacgccc gggatcggcg tcaggctcat cgccgttttc accatatagg
     8941 tgcggctgca cattgctggg actaccagga agataaatgc catcgagttt cggcaaaagt
     9001 tgttcaagta acgacggttc cgccagcgca tgtggtagcg caattggcaa gccgcctgcg
     9061 tggatgatgg cattcaggta cttttcttgc agagtctggg tcgcatgacc cttaagcctg
     9121 ttcctgcaca ttacgacacc gataaccgga ttgttcatta tatttttcat gctcaatctc
     9181 acaaagtgga ctaaattatc gccattactg cgctttaaat gacctttatg ttcaatattt
     9241 tttcaatcta gcagtgggtt ttcatttttg caaactaaat ttaacattta gcaaacattt
     9301 agtttgcata cagattcgaa tggtggtcat tatattttac gctttgataa cgagcggaaa
     9361 acaaaccaaa ggcgaagaat catggaaacc aatatcgttg aagtagagaa ctttgttcag
     9421 cagccagaag agaggcgggg tagcgccttt acgcaggaag tgaaacgcta cctggagcgc
     9481 tacccgaata cgcaatatgt tgatgtttta cttactgatt tgaatggttg cttccgtgga
     9541 aaacgtatcc ctgtttcaag cctgaagaag ttggagaaag ggtgttattt cccggcctcg
     9601 gtgtttgcaa tggatattct gggcaacgta gtagaagaag cgggtctggg tcaggaaatg
     9661 ggcgagccgg atcgtacttg tgttcctgtt ctcggttcct taaccccttc tgccgccgat
     9721 ccagagttta tcggtcagat gctcctgacc atggtcgatg aagatggcgc tccctttgac
     9781 gttgagccgc ggaacgttct taaccgcctg tggcagcagc tacgccagcg cggattgttc
     9841 ccggttgtag cggtagagct ggagttctat ttactggatc gtcagcgcga cgctgaaggg
     9901 tatctgcaac cgccctgcgc gcccggcacc gataaccgca atacacaaag ccaggtttac
     9961 tccgttgata acctcaacca cttcgccgac gtgctcaatg atattgatga actggcgcag
    10021 ttacagttaa ttcccgccga cggtgcggtc gccgaagcct cgccgggtca gtttgaaatc
    10081 aacctttacc ataccgataa cgtgctggaa gcctgcgatg atgcgctggc gctaaaactc
    10141 ctggtgcgtc tgatggcaga aaagcataag atgcactcca cttttatggc gaagccgtat
    10201 gaagagcacg cgggcagcgg gatgcatatc catatcagta tgcaaaataa tcgtggcgag
    10261 aatgtgcttt ctgacacaga aggcgaagat tcgccgctgc tgaaaaagat gctcgccggg
    10321 atgattgacc tgatgccgtc gtcgatggcg ttgctggcac caaacgtgaa ctcgtatcgc
    10381 cgcttccagc cgggaatgta tgtgccgacg caggcgtcgt ggggccataa caaccgcacc
    10441 gtcgccctgc gtattccgtg cggcgaccgt cataatcacc gcgtggaata tcgcgtggcg
    10501 ggtgccgatg ccaacccata tctggtgatg gcagcgattt ttgccggtat tttgcatggc
    10561 cttgataacg agctgccgtt gcaggaagaa gtcgaaggca acgggctgga acaggaaggc
    10621 ttacccttcc cgattcgcca gagcgatgcc ctgggtgagt ttatcgagaa tgatcacctg
    10681 cgccgctatt taggcgaacg gttttgccat gtgtatcacg cctgtaaaaa tgatgagctg
    10741 ttgcagtttg agcgcctcat cacagaaaca gaaattgagt ggatgttgaa aaacgcatag
    10801 taccccttgc ggggttgttt catcgacctg agcggagcca ggcctgacat ttgctggtaa
    10861 tccagacatt tcccggatgt cgcgttatgg agcgcaggcg gcaacgggct tccgttaacg
    10921 attaagactg tgacgaggaa atgagatgat gccgatgatt aaatccccgc atggcgaggg
    10981 aggctgcgta tgcgcccctc ctgctactga ctggactccg tcccctttgc ttcccttgct
    11041 aaacaggttt gatttcaggt cgacacgacc gcaaacctta ttacgcaggg ggaggcagca
    11101 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    11161 aaatcactga aattgtggca ggtggtgatg atgggtctgg cctatctcac gccgatgacc
    11221 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    11281 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    11341 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    11401 tttatggtcg gctggtcatc gctgctggat tatctctttt tgccgatgat caacgtcttg
    11461 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtagta
    11521 accttcgtcg ccattttaac cgccgcgaat ctgaagagca tcaacctggt cgctaacttc
    11581 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtagtt
    11641 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    11701 gagaacgcgc acctgatccc gattattacc ggggcgacga ttgtctgttt ctccttcctc
    11761 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    11821 aaagccatct tcctgacggc ggtctacggt ggcgttatct ttatcgcggc gtcgttcttt
    11881 atgcagctgt tctttcccga tatcagccgc tttaaagatc cggatgccgc actgcctgaa
    11941 attgcgctgt acgtcggcgg caagctcttc cagtcgattt tcctctgcac cacgtttgtg
    12001 aacacgttag cctctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12061 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    12121 ccggcactca acgtcattat ggtcgggatt gtcgcgttgt cggcgctgtt cttcgattta
    12181 gttaccgcca cagcgttaat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    12241 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    12301 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaacctc
    12361 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttatgg
    12421 tatctgatcc gccgctatcg caaagtgccg ttgtatgatg gtgacagaac gccggtgagt
    12481 gaaacgtaat agtcggatgc gcggcgcatt atcctgccgg agggtaatgc accgcgccat
    12541 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    12601 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    12661 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagac gctgaacgca caccgctggc
    12721 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    12781 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    12841 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    12901 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    12961 taatgggttt ctcacgatcg taccgctctg ctcctcgctg agttgttctg tgagttatag
    13021 ttatagagga agcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13081 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    13141 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    13201 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    13261 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    13321 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    13381 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    13441 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    13501 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    13561 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    13621 cttcgacagc ctgaaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    13681 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    13741 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    13801 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    13861 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    13921 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    13981 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14041 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    14101 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    14161 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    14221 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    14281 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    14341 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    14401 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcac ggttgatgga
    14461 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    14521 cttccgtcct ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    14581 cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcggcgcg
    14641 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattgccca ttttgccgct
    14701 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    14761 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    14821 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    14881 ttcgttggct tcagcttgag ctatttcacc cctcatgcgc cgttacaagg cgcgtcactg
    14941 tggaatgcct gggtgttctg gtttaacggc ctgatccact gggattttgg tgtttccagc
    15001 attaacggtc aaccgattgc cgaacagtta aaagaggtct tcccggcgac gatggaactg
    15061 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    15121 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    15181 atcccggtgt tctggctggc gcttctgttg acgctgtttt gttcactcac gctaggttgg
    15241 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    15301 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    15361 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    15421 ctgatgcgta tcagcactat cgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    15481 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    15541 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    15601 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    15661 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    15721 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    15781 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    15841 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcggggctgg ctgtactgtg
    15901 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    15961 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16021 cggacgcgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16081 tgtggtgacg cttgccgcga cgatctgtgg cctagtactg ggcaccttcg ccggggcgac
    16141 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg caatcccttc
    16201 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgct
    16261 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    16321 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    16381 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    16441 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    16501 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    16561 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    16621 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    16681 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    16741 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    16801 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    16861 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    16921 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    16981 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17041 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    17101 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    17161 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgacc
    17221 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    17281 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    17341 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    17401 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    17461 agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg gcgctatccc actgctggaa
    17521 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    17581 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    17641 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    17701 ccgctaccgg accggctggt ttcgccgtca gaccgtagaa gcggtaaaac ccttgagctt
    17761 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    17821 gctggcaaaa atgctggcgg gaatgataga gcctaccagc ggcgagttat tgattgacga
    17881 tcatccactg cattttggcg attattcctt ccgcagtcag cgtattcgca tgatttttca
    17941 ggatccttcg acctcgttga atccccgtca acgtatctcg caaattctgg attttccact
    18001 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18061 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    18121 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    18181 tgaagcactc gcttcactgg atatgtcgat gcgttcgcag ttgattaatc tgatgctgga
    18241 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    18301 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    18361 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    18421 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    18481 ccagattatc cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    18541 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    18601 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    18661 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    18721 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    18781 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    18841 agattattct gcaggaactg aaccgccgat caccctcacc ctgaaagggc gagggggccg
    18901 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    18961 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19021 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttagtg
    19081 taaggggaaa agcgcccacc ctgccgcagc ctgctccggt cggacctagc aactatagct
    19141 actcacagcc aggttgatta taataaccat ttatctgttc gtactgttta ctaaaacgac
    19201 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    19261 ctttccggta agcgcattct ggtaaccggt gttgccagca aactatccat cgcctacggt
    19321 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    19381 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    19441 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    19501 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    19561 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    19621 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    19681 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    19741 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    19801 gttaacgcca tctctgctgg tccgatccgt actctagcgg cttccggtat caaagacttc
    19861 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtaccgt tactattgaa
    19921 gatgtgggta actccgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    19981 gtggtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga attgaaataa
    20041 tcattctgtt ggtaaatatt gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    20101 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    20161 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    20221 tatcatgaaa cgcaatccag tactgcggag tatgacgttc tgcctctggt cccggaagcc
    20281 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagaaac gctagctccc
    20341 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg aattgttccc tgaccagctt
    20401 accgtgaccc gttcacagac cttcaccgct tatgaacgct tgtctacggc cctgacggtt
    20461 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    20521 cccgggcctg attcctctag ggaaagcaat cataggttgg cacaaatcac gcaatatgcc
    20581 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    20641 gtcggtctta ctgccaggga ctgtgtaatc attaaccaaa tcattggttt tattggcttt
    20701 caggcgcgga ccattgcgac atttcaggct tatctcgggc acccggtacg ctggttaccc
    20761 ggtctggaaa tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    20821 cgaagcagct atgaggtgga aaaactacct gaagagtaca caaaaagttc aacagcagaa
    20881 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaagg
    20941 ttgttaaaca gtacacgggt taatacacag ccggataata agcttgcggc gttgttatgc
    21001 gcgcggataa atggcagtcc tgcttgtttt gccgcctgta tggattcatc aaatgaatat
    21061 aaaaaaatca gcccccttct gcgcaagggc gaaaatgaaa ttaaccaatg ggctgaccgt
    21121 cattctgttg agcacgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    21181 tttagcaccg cccagttcag ccctttactc gaacacgaac aatcatcaac gcagattatt
    21241 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    21301 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    21361 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    21421 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    21481 cttcctggaa gtcgacgcgc aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    21541 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    21601 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    21661 tgaccgtctg gccatcgttc ctgatcatcc actcttaaaa gacgccattc cttgccgcgc
    21721 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    21781 tcatccgctg aaaggcgatc gttctttcta tgctgaattg acacaataca tcacttttgg
    21841 tgacgaccac tttgtaccgt ggtgggttac acttgcgcgc cataatctgg aaaaagaagc
    21901 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgaccgc
    21961 gctggatttt gtcaccatcg acagtgccag cacagaagat atggatgacg cccttttcgc
    22021 taaggcgttg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22081 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    22141 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    22201 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcgccat
    22261 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcaaagc tggtgtatga
    22321 ccaggtttct gactggctgg aaaataccgg tgactggcag cctgaaagtg aagcaattgc
    22381 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    22441 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    22501 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    22561 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    22621 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    22681 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    22741 tgaactggac gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    22801 tgctgaaatt agcactgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    22861 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    22921 tatcaaaggc gaaactgcga cgcgcccaca ggatgaaatc actgtccaaa tggccgagcg
    22981 tcgccgtctc aaccggatgg cagaacgtga tgttggtgac tggttatacg cacgcttcct
    23041 gaaagacaaa gccgggaccg acacccgttt cgcagcggaa attgtcgata tcagccgtgg
    23101 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    23161 acacgctatg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    23221 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    23281 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggcct a
//
LOCUS       DAJTNN010000052        23033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTNN010000052 DAJTNN010000000
VERSION     DAJTNN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.052"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..565)
                     /locus_tag="OO150_001789"
     CDS             complement(<1..565)
                     /locus_tag="OO150_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5515962.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGH"
     gene            complement(784..1761)
                     /gene="epmA"
                     /locus_tag="OO150_001790"
     CDS             complement(784..1761)
                     /gene="epmA"
                     /locus_tag="OO150_001790"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5515963.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2086..3894
                     /gene="frdA"
                     /locus_tag="OO150_001791"
     CDS             2086..3894
                     /gene="frdA"
                     /locus_tag="OO150_001791"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5515964.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3887..4621
                     /gene="frdB"
                     /locus_tag="OO150_001792"
     CDS             3887..4621
                     /gene="frdB"
                     /locus_tag="OO150_001792"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5515965.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4632..5027
                     /gene="frdC"
                     /locus_tag="OO150_001793"
     CDS             4632..5027
                     /gene="frdC"
                     /locus_tag="OO150_001793"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5515966.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5038..5397
                     /gene="frdD"
                     /locus_tag="OO150_001794"
     CDS             5038..5397
                     /gene="frdD"
                     /locus_tag="OO150_001794"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5515967.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5460..6593
                     /locus_tag="OO150_001795"
     CDS             5460..6593
                     /locus_tag="OO150_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5515968.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6682..7215
                     /gene="blc"
                     /locus_tag="OO150_001796"
     CDS             6682..7215
                     /gene="blc"
                     /locus_tag="OO150_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5515969.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7212..7529)
                     /gene="sugE"
                     /locus_tag="OO150_001797"
     CDS             complement(7212..7529)
                     /gene="sugE"
                     /locus_tag="OO150_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5515970.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7711..7857)
                     /gene="ecnB"
                     /locus_tag="OO150_001798"
     CDS             complement(7711..7857)
                     /gene="ecnB"
                     /locus_tag="OO150_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5515971.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7968..8093)
                     /gene="ecnA"
                     /locus_tag="OO150_001799"
     CDS             complement(7968..8093)
                     /gene="ecnA"
                     /locus_tag="OO150_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5515972.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8145..8711)
                     /gene="efp"
                     /locus_tag="OO150_001800"
     CDS             complement(8145..8711)
                     /gene="efp"
                     /locus_tag="OO150_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5515973.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8753..9802
                     /gene="epmB"
                     /locus_tag="OO150_001801"
     CDS             8753..9802
                     /gene="epmB"
                     /locus_tag="OO150_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5515974.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            10176..11045
                     /locus_tag="OO150_001802"
     CDS             10176..11045
                     /locus_tag="OO150_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5515975.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(11248..11601)
                     /locus_tag="OO150_001803"
     CDS             complement(11248..11601)
                     /locus_tag="OO150_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5515976.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11739..13385)
                     /gene="groL"
                     /locus_tag="OO150_001804"
     CDS             complement(11739..13385)
                     /gene="groL"
                     /locus_tag="OO150_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5515977.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13429..13722)
                     /locus_tag="OO150_001805"
     CDS             complement(13429..13722)
                     /locus_tag="OO150_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5515978.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13998..15254
                     /gene="yjeH"
                     /locus_tag="OO150_001806"
     CDS             13998..15254
                     /gene="yjeH"
                     /locus_tag="OO150_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5515979.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPCAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRSASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGIIT"
     gene            complement(15270..15746)
                     /gene="fxsA"
                     /locus_tag="OO150_001807"
     CDS             complement(15270..15746)
                     /gene="fxsA"
                     /locus_tag="OO150_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5515980.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            16083..17519
                     /gene="aspA"
                     /locus_tag="OO150_001808"
     CDS             16083..17519
                     /gene="aspA"
                     /locus_tag="OO150_001808"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5515981.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17637..18938
                     /gene="dcuA"
                     /locus_tag="OO150_001809"
     CDS             17637..18938
                     /gene="dcuA"
                     /locus_tag="OO150_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5515982.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPNMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            19054..19392
                     /gene="cutA"
                     /locus_tag="OO150_001810"
     CDS             19054..19392
                     /gene="cutA"
                     /locus_tag="OO150_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5515983.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19368..21065
                     /gene="dsbD"
                     /locus_tag="OO150_001811"
     CDS             19368..21065
                     /gene="dsbD"
                     /locus_tag="OO150_001811"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5515984.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNHALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            21102..21677
                     /locus_tag="OO150_001812"
     CDS             21102..21677
                     /locus_tag="OO150_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5515985.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKQLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21784..21859
                     /locus_tag="OO150_001813"
     tRNA            21784..21859
                     /locus_tag="OO150_001813"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21817..21819,aa:Phe,seq:gaa)
     gene            22057..>23033
                     /locus_tag="OO150_001814"
     CDS             22057..>23033
                     /locus_tag="OO150_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5515986.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGTGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQTVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENVMNAS"
BASE COUNT         5741 a         5945 c         5699 g         5648 t
ORIGIN      
        1 aatgcccacc atttaataac aaaatagtaa tgcttactaa cagcaatact aaattcagac
       61 acattactgc aataccgccc accgcagtaa tgcgggcaat tttattaatc ccctttgaag
      121 caacgacggt gaccagaatc atccatgcta ctgccagcag accaaccacc tgcgtaggct
      181 ccagtccggc aatacgccag tgctgggtca tgtcgctacc atagaggaat gttgagaacg
      241 gtacccaaac tttcgcggag gtactcacca tccagatgat ataagaggaa aaccacataa
      301 acgtaccaat gaaggcaaaa cgtggtccga cactattatt catccaggaa tagataccac
      361 cttcttcttt gcgataagca gctcccattt cagccatcat taaggcgaat ggaataaaga
      421 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      481 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      541 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      601 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      661 atacccggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      721 catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      781 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      841 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      901 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      961 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
     1021 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
     1081 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
     1141 ttctttgcca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
     1201 gtcttcttcg gtatcagcaa cgttactcaa atccagtttc gctgcgactt cccgcagttg
     1261 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
     1321 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1381 caaccggtac atatcatagt gcggtcgata ccactcaagc atagtgaact cagggttgtg
     1441 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1501 ggcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1561 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1621 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcag caaagaaacg
     1681 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1741 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1801 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1861 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1921 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1981 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     2041 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     2101 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     2161 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     2221 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     2281 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     2341 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtgggggtg cccatggagc
     2401 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2461 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2521 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2581 ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt gcagatccgt
     2641 gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg tttatcgtta caacaccaac
     2701 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2761 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2821 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2881 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2941 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     3001 tccacgccgc gtggcgatgt ggtttatctc gacttgcgtc acctcggcga gaaaaaactg
     3061 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     3121 aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat cgaaaccgat
     3181 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     3241 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     3301 atggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagaggca
     3361 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc
     3421 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctag caatggaaga aggttgcggt
     3481 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3541 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3601 accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3661 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3721 gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3781 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3841 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
     3901 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3961 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     4021 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     4081 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     4141 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     4201 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     4261 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     4321 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4381 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4441 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
     4501 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4561 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4621 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4681 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4741 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggacgcct
     4801 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4861 tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4921 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4981 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     5041 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     5101 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     5161 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     5221 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     5281 cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc
     5341 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtatcgttac aatctaacgt
     5401 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5461 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc
     5521 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5581 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5641 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5701 tcagcaaaac atttacgggc gtgcttggtg gcgatgctat tgctcgaggg gaaatcaagt
     5761 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5821 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcagatg ccggatgaag
     5881 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5941 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     6001 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     6061 accatacgtg gattaatgta ccgcccgcag aagaaaagaa ttacgcctgg ggatatcgcg
     6121 aaggcaaggc agttcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     6181 cgaccattga agatatggcc cgctgggtgc aaagcaattt aacccccctt gatatcaatg
     6241 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     6301 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     6361 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     6421 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     6481 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     6541 ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca
     6601 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     6661 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
     6721 tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtgacagt ggtgaataat
     6781 ttcgacgcta aacgctatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
     6841 gaacgtggac tggaaaaagt caccgcaaca tacagtctgc gtgatggcgg cggcctgaat
     6901 gtcatcaata aaggctataa ccctgacaga gaaatgtggc agcagagtga agggaaagcg
     6961 tactttaccg gcgcaccaac acgcgctgcg ctgaaagtgt cattctttgg tcctttctat
     7021 ggcggttata acgtcattgc acttgatcgg gaataccgcc atgcgctggt ttgcggcccg
     7081 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
     7141 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
     7201 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
     7261 agactcgcca ggcgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatagcc
     7321 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctactgg taacgatttc
     7381 atcgcccagg caagtagcgc catactgaca atcatcgccg tcacggtaat aacactcggc
     7441 gtcaaacgac taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
     7501 ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg
     7561 gtgaaaagac gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
     7621 ggtggcaggg gaatttcaac tattacctta gtgactatcg cagaaaaaac aaacggcacg
     7681 acacagctgt cgtaccgtta ttgcttattg ctattgctgc gctttcgttg ctgcgccaga
     7741 aatcgcgtta ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt
     7801 taatactgtt gaaagcacca gaacagaaaa gatcgctgca attgtcttct tcaccataac
     7861 gtcttccttt taggtaatgt tatttatgtt tgcctatagc aaattaataa tagacaacat
     7921 cgccaaaaat gacggaaatc tgatgatttt tcggaagaga agaaaaatta gctggcagcg
     7981 cgagagatgg agttgccgag atgtttgatg tcttcgccga aaccgcgagc ggtgttacag
     8041 cccgtgagca gcgtactggc aagcaaaaca agaacgataa ggcgtttcat catctctgac
     8101 ctgaataagt gatggtgcag cctgcaggcc gcaccacaac cgcattactt cacgcgagag
     8161 acgtattcac cagagcgggt atccactttg atgacttcgc cgatttgtac gaacagcgga
     8221 accttaacca cagcgccagt agacagggta gccggtttgc cgccagtacc tgcggtatca
     8281 cctttcaggc ccggatcggt atcaacgatt tccagttcaa cgaagttcgg cggagtaacg
     8341 gagatcggct gaccattcca cagagttacg atacactctg cctgatccag cagccattta
     8401 gcgttatcgc caattgcttt tgcatcagca gacagctgct cgaaagtttc gttgttcatg
     8461 aagtgccaga actcaccgtc gttgtacagg taagtcaggt tcatatcgac aacatcagcg
     8521 ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc gagtaccggt cagcagacga
     8581 cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt ttacgaattc actcgcttca
     8641 accgcgtaag gttcgccgtc taacatgatt ttaagaccag cacgaaaatc gttgctatag
     8701 tacgttgcca taaggccctc tgaaatttgt taattggtag ctaagccaca aaatggcgca
     8761 tattgtaacc ctaaataccc catccagaga agattggtta acgcaacttg ccgatgttgt
     8821 gaccgatcct gatgaacttc tgcgtctttt gaatatagac gcggacgaaa aactgttggc
     8881 cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc cgctcattta tcgatcgcat
     8941 ggagaaaggc aatccggacg atcctctttt gcgtcaggta cttacctcgc aagatgagtt
     9001 tgtcgtcgcg tccggattct ccaccgaccc actggaagaa cagcacagcg tagtgcctgg
     9061 tttgttgcat aaataccaca accgggcgct tttgctggtc aaaggcggct gcgcggtaaa
     9121 ttgccgctat tgcttccgtc gccacttccc ctatgccgaa aatcagggca acaagcgtaa
     9181 ctggcagact gcacttgagt atgttgctgc gcatccggaa ctggacgaga tgattttctc
     9241 cggcggcgat ccgctgatgg cgaaagatca cgagctggac tggttgctca cacaactgga
     9301 agccatcccg catatcaaac gtctgcggat tcacagccgt ctgccgattg tgatcccggc
     9361 acgcatcaca gatgcgctgg ttgaacgctt ttcacattct acgctgcaaa tcctgctggt
     9421 gaatcacatc aaccatgcca atgagataga tgaaacattc cgtcaggcga tggctaagtt
     9481 gcgccgggta ggcgttactt tgctgaacca gagcgttctg ttacgtgatg tgaacgataa
     9541 cgcacaaacg ctggcaaacc tgagtaatgc gttgttcgat gccggcgtga tgccgtatta
     9601 cctgcatgtg ctcgataaag tacagggcgc agcgcatttt atggtgagtg atgacgaagc
     9661 acggcagatt atgcgtgagt tgctgacatt ggtgtcggga tatctggtgc cgaaactggc
     9721 gcgagaaatt ggcggcgaac ccagcaaaac gccgctggat ctccagctac gccagcagtt
     9781 actgattaaa cagggaagtt aaacattaaa attgcctgat gcgctacgct tatcaggcct
     9841 acatcgtgca ttgaaattga atggatttgc aatgaactgt aggccggata aggcgtttac
     9901 gccgcatccg gcaagagcaa caaacgcaag tacagcaacc tgtcattaaa ctgagaaatt
     9961 taatctttgc ccgttgcata acctttaatt attcatatgc attgctgttg ttacttttta
    10021 tatagctaac accaataatt aacctttctt ttctgtttat atagagtgag cacggctatt
    10081 tatgttattt taacaactat tactaccaag cccatttatc tggcttataa attaaaatta
    10141 attatttaat tgagaaatgg ttagggagaa cctacatggc cataagcatc aagggtgtta
    10201 ataccggtgt tattcgcaag agtaataatt tcattgcact ggcgctgaaa attaaagaac
    10261 ctcgcaataa agaatcgttg ttctttatgt ctgtcatgga actgcgcgac ctgctgattg
    10321 cgctggaaag tcgtctgcat caaaagcata aactggatgc tgctgcacgc cttcagtatg
    10381 agcaagctcg cgataaagtc attaagaaaa tggcagaaaa catcccagaa attctggttg
    10441 atgagcttaa aaatgccgat attaatcgtc gggttaacac gctggaactt accgataatc
    10501 aaggcgaaaa tcttaccttc gtattaacgc tacatgacgg tagcaaatgt gagttagtcg
    10561 tcaatgagtt gcagattgaa atgctagcgc gagcaatcat tcatgcaatt aataatgcgg
    10621 aaatgcgtga actggcatta cgtattacct cactgttaga tttcctgccg ttatatgacg
    10681 ttgactgcca ggaaaatggc aatctggaat atgacaccta ttcccaaccg gaatggaaac
    10741 ataacttgtt cgaccattat ctggcggtgc tttaccgctt taaagatgaa agtggcaaag
    10801 aacaatttag cggcgcagtg gtgaaaacgc gtgaagcaac gccgggtaaa gaagtagagg
    10861 cgatcactcg ccgaatgctt gatttcagcc cacgactgaa aaaactcgcg ggtgtgccat
    10921 gtcaggtcta tgttcgcacc gtagcggcga ataacgccca accgctaacc caggatcaat
    10981 gcttacgcgc gctgcatcac ttacgtgttc aatccaccag caaaactgca ccacaggcga
    11041 aataatctca tctttgctaa tgcgaggctg gagccatcca gcctgaaatg actgataacg
    11101 tgatcgttgg tcatgccgga tgcggcgtaa acgccttatc cggcctacaa attctcaagc
    11161 attcaatcag ttgcaatgcc tgctgtaggc ctgataagcg aagcgcatca ggcaataata
    11221 cgtttgtcac ccagcctctg gatcacatca gttcgggcac ttataaacct gaccgataat
    11281 ctggctatcc gtcgggacaa aactggacaa cattccctgc gacgggctgc tgatgccata
    11341 aatcacgtta ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat
    11401 agaaccgccc tcttctccgt gttgcccgga aagccagttg ctttgcttac ctgtcgcagt
    11461 accaatcagc tggcactctg cgcctggctg ctcgtccaca atgcgtacac tctgaccggc
    11521 agcactcaat tcgttgctgg agctacaacc cgccatcagt agcgcggcac cgacaatccc
    11581 tgctaagtat tttacgtgca tgttattccc cataatcaat gagctggacg ccactcgcgt
    11641 cgtccgtgtc tgaatcttat actagaaaga tgaccaaaag aaaaaccccc agacatttct
    11701 gcccgggggt ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca
    11761 cccatgccgc ccataccgcc agcagcgcct aagtcagctg catcgttttt cggcaggtcg
    11821 gtaaccatgc actcggtggt gatcatcagg ccagccacag aagccgcgta ctgcagagca
    11881 gaacgggtta ctttggttgg atccaagata cccatgtcga tcatgttgcc gtattcttcg
    11941 gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa cagtgttagc aacaacagac
    12001 ggttcttcgc cgcagttcaa tacgatctga cgcagcggag cttccattgc acgcagtgca
    12061 actttgatac ccacgttctg gtcttcgttc tgaccacgca ggtcagccag tttagacgct
    12121 acgcggatca gcgcaacacc accaccagca accacgcctt cttctaccgc agcacgggtc
    12181 gcgtgcaggg catcttcaac gcgtgctttt ttctctttca tttcaacttc ggtagcagca
    12241 cctactttga taactgcaac gccgcctgcc agtttcgcta cgcgctcctg cagtttttca
    12301 cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga tctgagcaac acggccctgg
    12361 attgcagctt cttcacccac gccatcaatg atggtggtgg tgtctttgtt gatcacaaca
    12421 cgtttagcct gacccaggtc ttccagggtt gctttttcca gctccatacc gatctcttca
    12481 gagattacgg taccgccagt cagggttgcg atatcctgca gcatagcttt acggcgatcg
    12541 ccgaagcccg gagctttaac cgcagcaact ttcacgatgc cacgcatggt gttaacaacc
    12601 agagttgcca gcgcttcgcc ttcaacatct tcagcgatga tcagcagcgg tttacctgct
    12661 ttagcaacgg cttccagaac cggcagcatt tcgcggatgt tggagatttt cttgtcagcc
    12721 agcaggatga acgggctttc cagttctact gcgccagttt ccggcttgtt gatgaagtaa
    12781 ggagacaggt agccacggtc gaactgcata ccttcaacca cgtccagttc gtcctgcaga
    12841 ccggtaccgt cttcaacggt gataacgcct tctttaccga ctttgtccat cgcttcagcg
    12901 atcagtttac ctacggtttc gtcggagtta gcggagatgg taccaacctg agcaatcgct
    12961 ttagagtcag agcatggtac ggacagcgct ttcagttctt caactgcagc ggtaacagct
    13021 ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg cagcaactgc tttcagaccc
    13081 tcagtgatga tagcctgagc cagtacggtt gcagtggtgg taccgtcgcc tgcagcgtcg
    13141 ttcgctttag aggcaacttc tttcaccatc tgcgcaccca tattttcgaa cttgtcttcc
    13201 agttcgattt cacgagcaac ggaaacacca tctttggtga tggtcggtgc accgaaagat
    13261 ttatccagaa ctacgttacg gcctttcgga ccgagggtaa ctttcactgc atctgccagt
    13321 acgtttacgc cgcgcagcat tttcacacga gcgtcgttac cgaattttac gtctttagct
    13381 gccattatct ttattcctta aattcgtatg ttcagtgtcg tgcgaggatt acgcttcaac
    13441 aattgccaga atgtcgcttt cggacatgat caacacttct tcattgtcga tcttctcaga
    13501 tttcacaccg tagccatcgt tgaaaataac gatgtcgcca actttcacat ccagcggctt
    13561 cacttcgcca ttttcaagga tacggccatt gccgacagcc agcacttcgc cgcgggtgga
    13621 tttagccgct gcagagccgg tcagaacgat gccgccagca gatttagttt caacttcttt
    13681 acgcttgacg atcacgcgat catgcaatgg acgaatattc attgataact ctcctttgag
    13741 aaagtccgta tctgttatgg gtgacgccgg agcttacgtg gtttcccggc tggtgaccag
    13801 agaaatgggg atgaggattc gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc
    13861 acaaaatcgg gtgaaaaccc tgattcacct cacatttcat cgcaatttct tcatcgccgt
    13921 cataagcgaa tctgattgtg ctaccatcga aaatctacgc agttgcccaa aatttgggcg
    13981 caatcggata tcaacccatg agtggactca aacaagaact ggggctggcc cagggcattg
    14041 gcctgctatc gacgtcatta ttaggcaccg gcgtgtttgc cgttcctgcg ttagctgcac
    14101 aggtagcagg caataacagc ctgtgggcgt ggcccgtttt gattatctta gtgttcccga
    14161 ttgcgattgt gtttgcgatt ctgggtcgcc actatccctg cgcaggcggt gtcgcgcact
    14221 tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac cggctggttg tttttatcgg
    14281 tcattcccgt gggtttgcca gccgcgctac aaattgccgc cgggttcggc caggcaatgt
    14341 ttggctggca tagctggcaa ctgttgttgg cagaactcgg tacgctggcg ctggtgtggt
    14401 atatcggtac tcgcagtgcc agttccagtg ctaatctaca aaccgttatt gccggactta
    14461 tcgtcgcgct gattgtcgct atctggtggg cgggcgatat caaacctgcg aatatcccct
    14521 tcccggcacc tggtaatatc gaacttaccg ggttatttgc tgcgttatca gtgatgttct
    14581 ggtgttttgt cggtctggaa gcatttgccc atcttgcctc ggaatttaaa aatccagagc
    14641 gtgattttcc tcgcgcattg atgattggtc tgctgctggc aggattagtc tactggggct
    14701 gtacggtagt cgtcttacac ttcgacgcct atggtgaaaa aatggcggcg gcagcatcgc
    14761 ttccaaaaat tgtagtgcaa ctgttcggtg taggagcgtt atggattgcc tgcgtgattg
    14821 gctatctggc ctgctttgcc agtctcaaca tttatataca gagcttcgcc cgcctggtct
    14881 ggtcgcaggc gcaacataat cctgaccact acctggcacg cctctcttct cgccatatcc
    14941 cgaataatgc cctcaatgcg gtgctcggct gctgtgtggt gagcactttg gtgattcatg
    15001 ctttagagat caatctggac gctcttatta tttatgccaa tggcatcttt attatgattt
    15061 atctgttatg catgctggca ggctgtaaat tattgcaagg acgttatcga ctactggcgg
    15121 tggttggcgg gctgttatgc gttctgttac tggcaatggt cggctggaaa agtctctatg
    15181 cgctgatcgt gctggcgggg ttatggctgt tgctgccaaa acgaaaaacg ccggaaaatg
    15241 gcataatcac ataatccggc gtttcgacat taatcctggc gatcgtcttt atgatcaagg
    15301 cggtcgcgct catcatcctt tcgctggtac tcaccatcaa aagtattacc gccaccggtc
    15361 ccggcgctaa aaccgccgcc tggcatacga gaaaagcgca aatgcggcat caacttcact
    15421 gtcagatgct tttgcaccgg cggtaataaa agcagaagac cgaggaagtc ggtgaaaaag
    15481 cccggtaata aaagcagcaa accggcaatg atcagcgaaa cacttttaat catctccgcc
    15541 gctgggtttt cacccgccgc cattttttgc tgcatcagca cgaaattctt aaagccctgg
    15601 ttacgtacca gtgacatccc gataactgac gtgaatataa ccagcacgag ggtcagcaat
    15661 acccccaata catgggcaac ctgaataaag attgaaatct caatatagac ataaaggaaa
    15721 atggcaataa aaggtaacca gcgcaaaggt ttctcctgta atagcagccg gtaaaacccg
    15781 gctacctgaa tgggttgcga atcgcgttca gcttatattg tggtcattag caaaatttca
    15841 agatgtttgc gcaactattt ttggtagtaa tcccaaagcg gtgatctatt tcacaaatta
    15901 ataattaagg ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga aatcctcaag
    15961 cagcatatga tctcgggtat tcggtcgatg caggggataa tcgtcggtcg aaaaacattc
    16021 gaaaccacat atattctgtg tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc
    16081 acatgtcaaa caacattcgt atcgaagaag atctgttggg taccagggaa gttccagctg
    16141 atgcctacta tggtgttcac actctgagag cgattgaaaa cttctatatc agcaacaaca
    16201 aaatcagtga tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta
    16261 tggcaaacaa agagctgcaa accattccta aaagtgtagc gaatgccatc attgccgcat
    16321 gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt cccggtagac gtctaccagg
    16381 gcggcgcagg tacttccgta aacatgaaca ccaacgaagt gctggccaat atcggtctgg
    16441 aactgatggg tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac catgttaaca
    16501 aatgtcagtc cactaacgac gcctacccga ccggtttccg tatcgcagtt tactcttccc
    16561 tgattaagct ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg
    16621 aattccagga catcctgaaa atgggtcgta cccagctgca ggacgcagta ccgatgaccc
    16681 tcggtcagga attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa aatatccaac
    16741 gtaccgctga actgctgctg gaagttaacc ttggcgcaac agcaatcggt actggtctga
    16801 acacgccgaa agagtactct ccgctggcag tgaaaaaact ggctgaagtc actggcttcc
    16861 catgcgtacc ggctgaagac ctgatcgaag cgacctctga ctgcggcgct tatgttatgg
    16921 ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa aatctgtaac gacctgcgct
    16981 tgctctcttc tggcccacgt gccggcctga acgagatcaa cctgccggaa ctgcaggcgg
    17041 gctcttccat catgccagct aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat
    17101 gcttcaaagt catcggtaac gacaccactg ttaccatggc agcagaagca ggtcagctgc
    17161 agttgaacgt tatggagccg gtcattggcc aggccatgtt cgaatccgtt cacattctga
    17221 ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg catcactgct aacaaagaag
    17281 tgtgcgaagg ttacgtttac aactctatcg gtatcgttac ttacctgaac ccgttcatcg
    17341 gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg
    17401 aagtcgttct ggaacgcggt ctgttgactg aagcggaact tgacgatatt ttctccgtac
    17461 agaatctgat gcacccggct tacaaagcaa aacgctatac tgatgaaagc gaacagtaat
    17521 cgtacagggt agtacaaata aagaaggcac gtcagatgac gtgccttttt tcttgtgaac
    17581 agtaacttaa aaacaacaat ctaatatcaa cttgttaaaa aacaaggaag gctaatatgc
    17641 tagttgtaga actcatcata gttttgctgg cgatcttctt gggcgccaga ttggggggaa
    17701 taggtattgg ttttgcaggc ggattggggg tgctggttct tgccgctatt ggcgttaaac
    17761 ccggtaacat cccgttcgat gtcatttcca ttatcatggc ggttatcgcc gctatttctg
    17821 ccatgcaggt tgctggcggt ctggactatc tggttcatca gacagaaaag ctgctgcgcc
    17881 gtaacccgaa atacatcacg atcctcgcac ctatcgtgac ctatttcctg acaatctttg
    17941 ctggtactgg caacatctct ctggcgacac tgccagttat cgctgaagtt gcgaaggaac
    18001 aaggcgttaa accttgccgt ccgctctcta ctgcagtggt atccgcgcag attgcgatca
    18061 ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc cgtgatggaa ggtcatggca
    18121 tcagctacct ccatctgctc tccgtggtca tcccgtccac cctgctggcg gttctggtga
    18181 tgtcctttct ggtcactatg ctgttcaact ccaaactctc tgacgatccg atttatcgca
    18241 agcgtctgga agagggcctg gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg
    18301 gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt atctatgcaa
    18361 tcatcaacag cccaaacatg ggtctggttg aaaaaccgct gatgaacacc accaacgcaa
    18421 tcctgatcat catgctcagc gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg
    18481 acaacatcct caactccagc accttcaaag caggtatgag cgcctgtatt tgtatcctgg
    18541 gtgttgcgtg gctgggcgat actttcgttt ccaacaacat cgactggatc aaagataccg
    18601 ctggtgaagt gattcagggt catccgtggc tgctggccgt catcttcttc tttgcttctg
    18661 ctctgctgta ctctcaggct gcaaccgcaa aagcactgat gccgatggct ctggcactga
    18721 acgtttcacc gctgaccgct gttgcttctt tcgctgcggt gtctggtctg ttcattctgc
    18781 cgacctaccc gacgctggtt gctgcggtac agatggatga cacgggtact acccgtatcg
    18841 gtaaattcgt cttcaaccat ccgttcttca tcccgggtac tctgggtgtt gccctggccg
    18901 tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg atcaatcgcg gggcgttcac
    18961 gccccgcttt ctttcccgcc gactaacatc cttcccccgt acgttgtata gtgacctctc
    19021 tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag ttcgaatacc
    19081 gcgtctgtcg tggtgctatg tacggcacca gatgaagcga cagcccagga tttagctgcc
    19141 aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga tccccggcgc tacctctctc
    19201 tattactggg aaggtaagct ggagcaagaa tacgaagtgc agatgatttt aaaaactacc
    19261 gtatctcacc agcaggcact gctggaatgc ctgaagtctc atcatccata tcaaaccccg
    19321 gaacttctgg ttttacctgt tacacacgga gacacagatt acctctcatg gctcaacgca
    19381 tctttacgct gatcctgcta ctttgcagca cttccgtttt tgccggatta ttcgacgcgc
    19441 cgggacgttc acaatttgtc cccgcggatc aagcctttac ttttgatttt cagcaaaacc
    19501 aacatgacct taatctgacc tggcagatca aagacggtta ctacctctac cgtaaacaga
    19561 tccgcattac gccggaacac gcgaaaattg ccgacgtgca gctgccgcaa ggcgtctggc
    19621 atgaagatga gttttacggc aaaagcgaga tttaccgcga tcggctgacg cttcccgtaa
    19681 ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac ctaccagggc tgtgctgatg
    19741 ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg gtcgccaaca
    19801 acgcagcgtc acagcctgtg tctgtttcgc agcaagagca gcacaccgcg caattgccct
    19861 tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca tgcgtgctgc
    19921 caatgtaccc actgatttct ggcatcgtgc tgggtggtaa acagcggctc tccactgcca
    19981 gagcattgtt gctgaccttt atttatgtgc aggggatggc actgacttac acggcgctgg
    20041 gtctggtagt tgccgccgca gggttacagt tccaggcggc gctacagcac ccatacgtgc
    20101 tcattggcct cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc ttgtttactc
    20161 tacaactccc ctcttcgctg caaacacgtc tcacgttgat gagcaatcgc caacagggcg
    20221 gctcacctgg cggtgtgttt gttatggggg cgattgccgg acttatctgt tcaccatgca
    20281 ccaccgcacc gcttagcgcg attctgctgt atatcgccca aagcgggaac atgtggctgg
    20341 gcggcggcac gctttatctc tatgcgttgg gcatgggcct gccgctgatg ctaattaccg
    20401 tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc aaaaccgcgt
    20461 ttggttttgt gatcctcgca ctgccggtct tcctgctgga gcgagtgatt ggtgatgtat
    20521 ggggattacg cttgtggtcg gcgctgggtg tcgcattctt tggctgggcc tttatcacca
    20581 gcctacaggc taaacgcggc tggatgcgcg tggtgcaaat aatcctgctg gcagcggcat
    20641 tggttagcgt gcgcccactt caggattggg catttggtgc gacgcatacc gcgcaaactc
    20701 agacgcatct caactttaca caaatcaaaa cggtagatga gttaaatcat gcgctcgttg
    20761 aagccaaagg caaaccggtg atgttagatc tttatgccga ctggtgcgtc gcctgtaaag
    20821 agtttgagaa atacaccttc agcgacccgc aggtgcaaaa agcgttagca gacacggtct
    20881 tacttcaggc caacatcacg gccaacgacg cacaagatgt ggcgctgtta aagcatctta
    20941 atgtccttgg cctaccgaca attctctttt ttgacggaca aggccaggag catccacaag
    21001 cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc acatttgcgc gatcgccaac
    21061 cgtgaacgac acttgcagcg ggaaagacgg aggaaaatag cgtgcaacgt gaagatgtac
    21121 tgggagaagc cctgaaatta ttagaattac aagggattgc caacaccacg ctggagatgg
    21181 ttgctgaacg tgtggattat ccactggacg agctacgccg cttctggcca gacaaagagg
    21241 caatcctcta cgatgcgctg cgttatctta gccaacagat agatgtctgg cgtcgtcagc
    21301 tgatgctgga cgaaacgcaa accgccgaac aaaagcagct ggcacgttat caggcgttat
    21361 cggagtgcgt taaaaacaac cgctatccgg gctgtctatt tatcgctgcc tgtacgtttt
    21421 atcccgatcc tggccaccct attcatcaac tggccgatca gcaaaaaagc gcggcctacg
    21481 atttcaccca cgaactgtta accacactgg aagttgacga tccggcgatg gtagcaaagc
    21541 agatggaact ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt agccaggcgg
    21601 atgtcgacac tgcacatcgg ctggcggaag atatcctgcg cttcgcccgc tgccgccagg
    21661 gtggtgcatt gacctgacag aaacacagaa aagaagcgat ttgccgcaat cttaagcagt
    21721 tgaatcgctt ttactgaaat taggttgacg agatgtgcag attacggttt aatgcgcccc
    21781 gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg
    21841 attccgagtc cgggcaccaa attcatatca acggacctct acggaggttc gtttttcgtt
    21901 tcagaacgcc acgatttaag cgttctgcct acaaatcaat tctaccgaac tcaaccagat
    21961 tccccccaca tcaaccccat tgtgtgggta taattgcggg cataccccag ttcgacagaa
    22021 tttgtaccct ctttaacgca ttcagaaggc tgaactatgg cactgactga cgcaaaaatc
    22081 cgggctgcaa agcccactga caaggcttat aaactcactg acgggactgg catgttcctg
    22141 ctggtacatc ctaatggctc ccgttactgg cgtctccgtt atcgtattct gggtaaggag
    22201 aagactctgg cacttggtgt gtatccagaa gtttctctct ccgaagctcg tacaaaacgg
    22261 gatgaggccc gaaaactgat ttcggagggg gttgaccctt gcgaacagaa aagagctaaa
    22321 aaagtagtcc ctgatttaca gctctctttt gaacatattg cgcgacgctg gcatgccagt
    22381 aataaacaat gggcacaatc acacagcgat aaagtactca aaagcctcga gactcacgtt
    22441 ttccccttta tcggcaaccg ggatatcaca acactcaata ccccggatct gcttatccct
    22501 gttcgtgctg cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
    22561 tctgccgtaa tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat
    22621 atggctggcg cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc
    22681 ctgcctgaac ttttgtcgcg tattggcagt tacaaagggc aaactgtcac ccagcttgcc
    22741 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct
    22801 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc cctgccaggc
    22861 gtaaaattct cacaccgggg ctccaagatg cgaacaccac atcttgtgcc actcagcaaa
    22921 caggctgtag ccatactgac agaactacag acatgggctg gtgaaaatgg tctgatcttt
    22981 acgggagcac atgacccgcg taaaccaatc agtgaaaatg taatgaacgc atc
//
LOCUS       DAJTNN010000053        22917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTNN010000053 DAJTNN010000000
VERSION     DAJTNN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.053"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..730)
                     /gene="fsr"
                     /locus_tag="OO150_001815"
     CDS             complement(<1..730)
                     /gene="fsr"
                     /locus_tag="OO150_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5515987.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSVGAVLLAAALVGTSSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLTAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYM"
     gene            948..2600
                     /gene="ushA"
                     /locus_tag="OO150_001816"
     CDS             948..2600
                     /gene="ushA"
                     /locus_tag="OO150_001816"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5515988.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(2637..3116)
                     /gene="ybaK"
                     /locus_tag="OO150_001817"
     CDS             complement(2637..3116)
                     /gene="ybaK"
                     /locus_tag="OO150_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5515989.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3320..4114)
                     /locus_tag="OO150_001818"
     CDS             complement(3320..4114)
                     /locus_tag="OO150_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5515990.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4252..4593
                     /locus_tag="OO150_001819"
     CDS             4252..4593
                     /locus_tag="OO150_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5515991.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4808..7312)
                     /gene="copA"
                     /locus_tag="OO150_001820"
     CDS             complement(4808..7312)
                     /gene="copA"
                     /locus_tag="OO150_001820"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5515992.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPLW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNPALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7574..8506
                     /gene="glsA"
                     /locus_tag="OO150_001821"
     CDS             7574..8506
                     /gene="glsA"
                     /locus_tag="OO150_001821"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5515993.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAVVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8509..9801
                     /locus_tag="OO150_001822"
     CDS             8509..9801
                     /locus_tag="OO150_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5515994.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            9926..10333
                     /gene="cueR"
                     /locus_tag="OO150_001823"
     CDS             9926..10333
                     /gene="cueR"
                     /locus_tag="OO150_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5515995.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10334..10792)
                     /locus_tag="OO150_001824"
     CDS             complement(10334..10792)
                     /locus_tag="OO150_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5515996.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10789..11706)
                     /locus_tag="OO150_001825"
     CDS             complement(10789..11706)
                     /locus_tag="OO150_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5515997.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11852..12529
                     /gene="fetA"
                     /locus_tag="OO150_001826"
     CDS             11852..12529
                     /gene="fetA"
                     /locus_tag="OO150_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5515998.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12516..13295
                     /gene="fetB"
                     /locus_tag="OO150_001827"
     CDS             12516..13295
                     /gene="fetB"
                     /locus_tag="OO150_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5515999.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13358..14212)
                     /gene="cnoX"
                     /locus_tag="OO150_001828"
     CDS             complement(13358..14212)
                     /gene="cnoX"
                     /locus_tag="OO150_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5516000.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14273..15082)
                     /gene="ybbO"
                     /locus_tag="OO150_001829"
     CDS             complement(14273..15082)
                     /gene="ybbO"
                     /locus_tag="OO150_001829"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5516001.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15072..15695)
                     /gene="tesA"
                     /locus_tag="OO150_001830"
     CDS             complement(15072..15695)
                     /gene="tesA"
                     /locus_tag="OO150_001830"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR5516002.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            15666..16352
                     /locus_tag="OO150_001831"
     CDS             15666..16352
                     /locus_tag="OO150_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5516003.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16349..18760
                     /locus_tag="OO150_001832"
                     /pseudo
     CDS             16349..18760
                     /locus_tag="OO150_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            19189..22917
                     /locus_tag="OO150_001833"
                     /pseudo
     CDS             19189..22917
                     /locus_tag="OO150_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         5288 a         6001 c         6477 g         5151 t
ORIGIN      
        1 ccatatagaa atatttcgag aaaatgagga ttaacagaat gctgactgcc agtaccactt
       61 tgttgcgcgg cagtggattg attatcgtcg ctttgggttt tcctttattc attcggtgct
      121 gtgccgagta ccaacggcta atttgcgcca acaccacgat cgccagcagt gccgcaagca
      181 caaaccaggc aacgttgcct ttgccataag gcgcgataat caccgccgtc agcaagggtc
      241 ccagggaact gccaaagttg ccgccgacct gaaagataga ttgcgccagg ccatgccgcc
      301 cgccggaagc catacgggct acgcgagaag attccggatg aaagaccgat gaactggtac
      361 cgaccagcgc cgccgccagc agaactgcgc caacactgcc cgccagcgca agcagcacca
      421 gaccacttaa ggtaaagcac atgccaattg gcaacgacca cggcatcgga tatttatcgg
      481 tccagtagcc gaccactggt tgcagtagcg aagaggcgag ctggaaggtg agggttatca
      541 tgccaatctg cataaatgtc agagaaaatt ctgactgaag cagaggataa atcgccagaa
      601 tcagcgattg gatcatgtcg ttcagcagat gtgagaggct gatagcacct aaaataccaa
      661 acgatgttcg ggccttggtc gttgacgcag ccgcgcccgc cacaggctgg gtttgttcac
      721 tcattgccat aggaaagtca ctttttcagg gttgcgatgt aaagaatgat cttatttgtg
      781 attattaccc gactaacata cctgtaagcg tcgcctgaag gaagtctcaa cgccgaatac
      841 agaatttcta atctggatgc agatttatct tcaccggacg cagacttgtc tattatgttg
      901 cgtcatacta tttttcaaca cgttgaaatc aggtcaggga gagaagtatg aaattattgc
      961 agcggggcgt ggcgttagcg ctgttaacca catttacact ggcgagtgaa actgctctgg
     1021 cgtatgagca ggataaaacc tacaaaatta cagttctgca taccaatgat catcatgggc
     1081 atttttggcg caatgaatat ggcgaatatg gtctggcggc gcaaaaaacg ctggtggatg
     1141 gtatccgcaa agaggttgcg gctgaaggcg gtagcgtgct gctactttcc ggtggcgaca
     1201 ttaacactgg cgtgcccgag tctgacttac aggatgccga acctgatttt cgcggtatga
     1261 atctggtggg ctatgacgcg atggcgatcg gtaatcatga atttgataat ccgctcaccg
     1321 tattacgcca gcaggaaaag tgggccaagt tcccgttgct ttccgcgaat atctaccaga
     1381 aaagtaccgg cgagcgcctg tttaaaccat gggcgctgtt taagcgtcag gatctgaaaa
     1441 ttgccgtcat tgggctgaca accgatgaca cagcaaaaat tggtaacccg gaatacttca
     1501 ctgatgtcga atttcgtaag cccgccgatg aagcgaagct ggtgattcag gagctgcaac
     1561 agacagaaaa gccagacatt attatcgcgg cgacccatat ggggcattac gataatggtg
     1621 agcacggctc taacgcaccg ggtgatgtgg agatggcacg cgcgctgcct gccggatcgc
     1681 tggcgatgat cgtcggtggt cactcgcaag ctccggtctg catggcggca gaaaacaaaa
     1741 aacaggtcga ttacgtgccg ggtacgccat gcaaaccaga tcaacaaaac ggcatctgga
     1801 ttgtgcaggc gcatgagtgg ggcaaatacg tgggacgggc tgattttgag tttcgtaatg
     1861 gcgaaatgaa aatggttaac taccagctga ttccggtgaa cctgaagaag aaagtgacct
     1921 gggaagacgg gaaaagcgag cgcgtgcttt acactcctga aatcgctgaa aaccagcaaa
     1981 tgatctcgct gttatcgccg ttccagaaca aaggcaaagc gcagttggaa gtgaaaatag
     2041 gcgaaaccaa tggtcgtctg gaaggcgatc gtgacaaagt gcgttttgta cagaccaata
     2101 tggggcggtt gattctggca gcccaaatgg atcgcactgg tgccgacttt gcggtgatga
     2161 gtggaggagg aattcgtgat tctatcgaag caggcgatat cagctataaa aacgtactga
     2221 aagtgcagcc attcggcaat gtggtggtgt atgccgacat gaccggtaaa gaggtgattg
     2281 attacctgac cgccgtcgca caaatgaagc cagattcagg tgcctacccg caatttgcca
     2341 acgttagctt tgtgacgaaa gacggcaaac tcaacgacct gaaaatcaaa ggcgaaccgg
     2401 tcgatccggc gaaaacttac cgtatggcga cattaaactt caatgccacc ggcggtgatg
     2461 gctatccgcg ccttgataac aaaccgggct atgtgaatac cggctttatt gatgccgaag
     2521 tgctgaaagc gtatatccag aaaagctcgc cgctggatgt gagtgtttat gaaccgaaag
     2581 gtgaggtgag ctggcagtaa tccgaaagtg ccggatattt gcatccggca caatgcttaa
     2641 tcgcggcggg cgatatcagc aaatttggca tcgaggatct tcgccagatc gcctgccgcc
     2701 agttcgatat ccagtccgcg cttgccgcca gaaacataaa tagtggcaaa ttcttgtgcg
     2761 ggggcgtcga taatcgtcgg cagacgtttt ttctgcccca gtgggctaat ccccccaacc
     2821 aggtatcccg tcgaacgctg cgcggccatc ggatcggcca tctcaacttt cttggcaccc
     2881 agcgcttttg ctactttttt aagatccagt tgacctgcga ccggcgtaac ggccactgcc
     2941 agatgtttca tatcaccgtt cactgccacc agtagcgttt tgtagacttg atccggattc
     3001 aaacctaatt ttttgacgac ttcatcgcca aaattggttt cagccggatc gtgctcgtag
     3061 gtatggattt gaaacgaaat cttgtttttt tcgagtaatt taactgcggg tgtcatggta
     3121 attttcctgc cttaaacaaa aaaacgaagc aagcatacgc ctgatggcgg tccaaaaaaa
     3181 agagtcatct tgcctaagag tattggcagg atggtgagat tgagcgacaa tcgagttaca
     3241 ccgtcgctta aagtgacggc ataataataa aaaaatgaaa ttcctctttg acgggccaat
     3301 agcgatattg gccatttttt tagcgcaaca tttgcggcaa attcccttct ccatacaggt
     3361 gtagtgcacc gaccgcgacc acatatcgcc ccggcggcat ggcgcgtaat ttatcccgcc
     3421 aggcgagatt tcgctgatgc atcagcacat cgtacagcga ctgactgaac gtattgggca
     3481 gcgttatatc attattttgc ggaggtgcat tcagccacca gctcatcatt tgttgcagca
     3541 accgtgcgtt ggtatgccag tgggtcagcg tatcgtccag cagcgccagt cctttgtcag
     3601 ggagctggag caacatggta atctggtttt cagccccttc cagttcaatc acgggtttat
     3661 gttgttgctt cgccgcctgc aataactggt aatcgatacc gtattctgcc cgcagcccca
     3721 gtttttgtgc ctgcgtcgcc tgaagaacca tcgcgatttg ccacagcggt tgagtagaaa
     3781 agagcgacgg agaaatgccc atctcctggc taatgtgctg cagattttgg agttgctcct
     3841 cgttaatgcg ctcttccagc gcctcgcaag taggcaaatt agcaaaaggc gtatcgctgg
     3901 tggaaacatc cgcctcgacg atcagcgcat cggcgttttt gagctttttg agcaaacggg
     3961 tgggcagggg agtcatatcg tggctaccca tatgaatact gccaatcaga tgaaaatggc
     4021 gattgccggg aagggtgata tcgatggcag gccaggtgta atgattaccg cgcagcgcgg
     4081 cccaaagcgt ttttacccgg tacaacagat ccatacgacc tccctttgtg aaatatcatg
     4141 ctagcgcgcg gtgagggatg gcgcaaatgg agttggggac ttgtttaatg tgtttgtatg
     4201 attcagtatg ttcttgcatc gctattcaca gggaagcaac agttaaaaac catgaaacag
     4261 gcaacaagaa aaccgacgac acccggagat attcttctct atgaatatct ggaaccgctc
     4321 gatttgaaaa tcaatgagtt agcagagttg ctgcatgttc atcgtaatag cgtcagtaca
     4381 ctgatcaata ataatcgtaa actcaccact gagatggcat tccgtctggc gaaagttttt
     4441 gataccacag tcgatttttg gctaaacctc caggcggcag ttgatctttg ggaagttgaa
     4501 aacaacatgc gcacccagga agaattggga cggattgaaa cagtggctga atatttggca
     4561 cgccgtgaag agcgtgcaaa aaaggtcgcg taaggccaaa acatcgcttc atctcgtagg
     4621 ccggataagg cgttcacgcc gcatccggca tccgagcacc atagcctgat gcgacgctta
     4681 cccgtcttat caggcctaca aacctgtgcc tgaaccgtag gccggataag gcgtttacgc
     4741 cgcatccggc agtcatgcgt cgatgccaaa tgcgccgccc taaagcagcg catccgcaat
     4801 gacgtactta ttccttcggt ttaaaccgca gcaaccggtt ggcgttactc actacggtaa
     4861 tcgacgagag cgccattgcc gctccggcaa ctaccgggtt aagcagtgtt ccagtaaacg
     4921 gccacaaaat accagcggcg accggaatac caatgctgtt gtagataaac gcaccgagca
     4981 ggttctgctt catattgcgc agcgttgcgc gggaaatcgc gagcgcatct gcaacaccca
     5041 tcaggctatg gcgcatcagg gtaatcgccg cggtttcaat ggcaacatca ctgccgccac
     5101 ccatcgcaat accgacatcc gcctgagcca gcgctggcgc gtcgttaatg ccgtcgccca
     5161 ccattgccac ctgacgtcct tcactttgca gacgtttgat cgcttcggct ttaccgtctg
     5221 gcagcacccc ggcgattacc tcatcaatcc ctgcttcttt ggcgatcgca ttggcggtgg
     5281 ttgggttatc cccggtcaac atcaccagac gatatcccgc tttatgcagg cgttgcagcg
     5341 ccgccacgct atcactacgc aacggatcgc gtacagccag cagggctact actttcccgt
     5401 caatcgccag cagcacaggc gttgctcctt gcgatgcctg agctgaaata tcggcttcaa
     5461 tcgctttagt atcgacttgc tggtcattta acagcgccgg attacccagc aataacgcat
     5521 gaccttcagc ttcaccgctt acgcccagcc cgcgcaatgt gcggaaaccg ttgacctgcg
     5581 gcagctgcat atcgcctgct ttatcgagga tcgctcgtgc cagcggatgg ctggaacctt
     5641 gctccagcgc cgccgccaga cgcaatgctt gcgcttcatc aacatcagca aatgttttta
     5701 ctgcgacaac ctgcggcttc ccttcggtca gcgtcccggt tttatcgaac acgacggtat
     5761 cgagtgtact ggcgcgttgc agcgcgtcgg cgtcccgcac cagcacgcca aactcagccg
     5821 cccgcccgac gccggaaata atcgacattg gcgtcgccag ccccagcgca cacggacagg
     5881 caataatcag caccgtggtg gctatcacca gggtatagac aatctgcggt gccggaccaa
     5941 agaaatacca gattgctgca ctgatcagcg caatcaccac tactaccggc acaaatacgg
     6001 ctgagatttt atccgccagc tgaccgattt ctggcttgct gctctgggcc tggcgcacca
     6061 tgcgaataat ccttgacagc gtggtatggc tgccaaccgc actggcgcga aatagcacgc
     6121 taccgtcctg taccactgtc ccggcatgga cgctttcgcc ttcgcctttt tgctgcggga
     6181 ttggttcgcc cgtcagcatc gcttcatcca gccatgcttc gccctgggta atctcgccat
     6241 ctaccggcac gcgatcgccg gtcgtcaggc gcagtaacat ccctggctga acttccgcca
     6301 gcggcacgct tttttcaccc tctgcggtga ccactcgcgc cgtcggtggg gttaaatcga
     6361 gtaatttctc cagcgcctta gaagaacgct gacgcgcgcg cgcttccagc atatggccga
     6421 gattaatcag accgataatc atcgcactgg cttcatagta aagatgccgc gcttccatcg
     6481 ggaaccacag cggccacagg ttgacgctca tcgaatagag ccacgccaca cccgtaccca
     6541 gcgccaccag cgtatccatc gtcgccgcac cgttcagcag gcttttccac gcgctgcggt
     6601 aaaaatgccc gccggcgaaa accattaccg ccagggttat caggccgata actaaccaca
     6661 ggctgcggtt gtcagcggtg accatcatgt tatcgccgat catcccccag accattaccg
     6721 gaatacccac cgccagtgcg acaatcgcct gccagcggaa gcgcttcatc gtagcgacgg
     6781 cggtttcctg ctggcgctcg cggcgtttcg cgtcatcttc aatcgcttcc gcgccgtagc
     6841 ccgctttttc caccgcctgc actaaatctt gtggggaggc actgcccatc accagcgcag
     6901 tacgctccgc caggtttacc cgtgcctgag tgacgcccgg tacgctttgc agcgcatttt
     6961 gtacgcgggt gacacagctg gcgcagctca tgccgctcag cagcaactgc tggctgtcat
     7021 cgtcatcggc ggtcgctgcc ggaagcgcct cagaaaccgc tgtcagtgct tccgacggga
     7081 ttgatgactc cgccagcggt ttagcctttg ggtggcttac agatgcgtca taacccgctt
     7141 gtttgatggt ttcgatcagc tgttctgcac tggcagtccc ggtaacgtgc gcttcagtga
     7201 tagacacatc cgcttgttcc acatccgggc gctgttcgag actttctttc acgcgtttaa
     7261 cgcagtgacc gcaggacagg ccgtccaggg tcaggtcgat agtttgtgac ataaaacact
     7321 cctttaagac agttttgact ggctgtgata aaggttaaac cttccagcaa ggggaaggtc
     7381 aagaaattaa taaaccaggc gggtaaaagt ccgtaaagat taaaaaatcg gctcgatttg
     7441 catcaggatt agacatttat ttctttgttt tcctgtagtt aagttgctgg tgctaagtta
     7501 aatcaggatg cctgaaaatc ggcaccgggg tgaggaatta cctcccgcat ctataaaaag
     7561 gagttaacaa aagatgttag atgcaaacaa attacagcag gcagtggatc aggcttacac
     7621 ccaatttcac tcacttaacg gcggacaaaa tgccgattac attccctttc tggcgaatgt
     7681 accaggtcaa ctggcggtag tggctatcgt gacctgcgat ggcaacgtct atagtgcggg
     7741 tgacagtgat taccgctttg cactggaatc catctcgaaa gtctgtacgt tagcccttgc
     7801 gttagaagat gtcggcccgc aggcggtaca ggacaaaatt ggcgctgacc cgaccggatt
     7861 gccctttaac tcagttatcg ccttagagtt gcatggcggc aaaccgcttt cgccactggt
     7921 aaatgctggc gctattgcca ccaccagcct gattaacgct gaaaatattg aacaacgctg
     7981 gcagcgaatt ttacatatcc aacagcaact ggctggtgag caggtagcgc tctctgacga
     8041 agtcaaccag tcggaacaaa caaccaactt ccataaccgg gccatagcct ggctgctgta
     8101 ctccgccgga tatctctatt gtgatgcaat ggaagcctgt gacgtgtaca cccgtcagtg
     8161 ctccacgctc atcaatactg ttgaactggc aacgcttggc gcgacgctgg cggcgggtgg
     8221 tctgaatccg ctgacgcata aacgcgttct tcaggccgac aacgtgccgt acattctggc
     8281 cgaaatgatg atggaagggc tgtatggtcg ctccggtgac tgggcgtatc gcgttggttt
     8341 accgggcaaa agcggtgtag gtggcggtat tctggcggtc gtccctggcg tgatgggaat
     8401 tgccgcgttc tcaccaccgc tggacgaaga aggtaacagt gttcgcggtc aaaaaatggt
     8461 ggcatcggtc gctaagcaac tcggctataa cgtgtttaag ggctgatcat gatgaacacg
     8521 gaaggtaata acggtaacaa acccctcggt ctatggaatg tcgtttccat cggtattggg
     8581 gcaatggtgg gggcggggat cttcgcgctg ctggggcagg ctgcgttgct aatggaagcc
     8641 tcgacctggg tcgcctttgc ttttggcggt attgtggcga tgttttccgg ttatgcctat
     8701 gcgcgtctgg gggcgagcta tcccagcaat ggcggcatta tcgacttctt tcgtcgcgga
     8761 ttaggcaacg gcgtcttttc gctggcgctc tcgttactgt acctgttgac gctggcggtg
     8821 agcatcgcca tggtcgccca tgcttttggc gcttatgccg tgcagttttt gcatgaaggc
     8881 agccaggagg agcaccttat tttgctctac gcgttgggga gcattgcggt gatgacgctt
     8941 ttcaactcct taagcaacca tgcggtaggg cggctggaag tgatcctcgt cggcattaaa
     9001 atgatgatcc tgttattgct gattattgcc ggtgtctggt cgctgcaacc agcgcatatt
     9061 tccgtctctg cgccccccag ctccggtgcg ttcttctcct gtattgggat aactttcctt
     9121 gcctatgcgg gctttggcat gatggcgaac gcggcggata aagtgaaaga tccgcaggtc
     9181 attatgccac gggcgtttct ggtggcgatt ggcgttacca cgttgcttta tatctcgctg
     9241 gcactggttt tgcttagcga tgtatcggca ttagagttag aaaaatatgc cgataccgcc
     9301 gtagcgcagg ctgcttctcc gctgctcgga catgtgggtt atgtgatcgt cgtcatcggc
     9361 gctttactgg cgacggcttc agccattaac gcgaacctgt tcgccgtgtt taacatcatg
     9421 gacaacatgg gcagcgaacg cgaactgccg aagctaatga ataaatccct gtggcggcag
     9481 agtacctggg gcaacatcat tgtcgtggtg ttgattatgc tgatgacggc ggcactgaat
     9541 ttaggctcac tcgccagcgt tgccagcgcc acctttttga tttgctacct ggcggtgttt
     9601 gtggtggcga tccgcctgcg tcatgatatt cacgcctcgt tgccgattct tatcgttggt
     9661 acgttggtga tgttgttggt gatcgttggc tttatctaca gtctgtggtc ccagggtagc
     9721 cgtgcgttga tatggattat tggctcactc ttactcagcc ttattgtggc aatggtcatg
     9781 aagcgcaata aaaccgtata acatctctct gtgcgcagta cttcctgtat tattgtggtg
     9841 gcggtcgata ttcgcactgg caaaaaaacg tgcttgaata tctgttgaaa ccctttaaca
     9901 aagcacagga ggcgttgcgc gaacgatgaa catcagcgat gtagcaaaaa ttaccggcct
     9961 gactagcaaa gccattcgct tctatgaaga gaaggggctg gtgacgccgc cgatgcgcag
    10021 cgaaaacggt tatcgcacct acacgcagca gcatctcaac gaactgacct tactgcgcca
    10081 ggcacggcag gtgggcttta acctggaaga gagcggcgag ctggtgaatc tgtttaacga
    10141 cccgcagcgg cacagcgccg acgtcaaacg gcgcacgctg gagaaggtgg cggagatcga
    10201 acgacacatt gaggagctgc aatccatgcg cgaccagctg ctggcactgg cgaatgcctg
    10261 ccctggcgat gacagcgccg actgcccgat tatcgaaaat ctctccggct gctgtcatca
    10321 tcgggcaggg tgattaagac gagacggctc ggatatgtag ggttatccct tctatcgcaa
    10381 tgacttcaac atgcgtacct gcaccgagat cctcgctggc gctgacaggc catgaactgt
    10441 caccgacgcg catatgaccg cgcccgttga ccagcggaga ttccagcaca aaacgtcggc
    10501 caatcagctg ctgcccgcgc tggtttaaat gactatcgct gtgcttttgt tcgcgcaccc
    10561 gccgcgacaa ccatttccac cacagccagg cggcgagcag cgtcaggatg gcaaacatta
    10621 ccccttgcca ctcccaaccc agcggcacca gccagaccac caggccggta atcaccgctg
    10681 ccacgccgct ccacaacaaa taaccatttc cgcccagcat ctcggctgcc agcagcaaac
    10741 cgccgagact gagccagaaa atatgtggat gaacgaccat taactccatc atggctgagt
    10801 ccgtttgttg gcgctgtctt tcaccagttc ggcaatcccg gcaatcgacc ccatcaggct
    10861 gctggcctct aatggcatca tcactacttt gctgttactg gaggaaccga tctgctgtaa
    10921 cgcttcggtg tatttctgcg ctacgaagta gttcaccgcc tgaatatcac cggaggcgat
    10981 agcttcagac accattttgg tggcgcgggc ttctgcttcg gcggaacgtt cacgcgcttc
    11041 agcctgtaaa aacgccgact gacgttcgcc ttccgctttc aggatttgcg actgtttttc
    11101 accttcggct ttgaggattt ccgcctgacg gatcccttcc gcttcaagaa tgtaagcgcg
    11161 tttggtacgt tccgctttca tctgcgcgtt cattgaagag ataagctctg ccggtgggcg
    11221 cacgtcgcga atttcaatac gggtgacttt aatcccccac gggttggtgg cttcatcgac
    11281 aatacgcagc aggcgtgagt tgatgctgtc gcgctgagag agcatttcgt caagttccat
    11341 tgaacccaac acggtacgga tgttagtcat ggtcaggttg atgatcgcca gctccagatt
    11401 gctgacttca taggcagcgc gtggcgcgtc aatcacctga ataaaacaga ctgcgtcgat
    11461 ggtaacgttg gcgttatctt tcgagataac ttcctgggaa gggatatcga gcacttgctc
    11521 catcatattg atcttgcgac caatgcgatc cataaacggc accaccagac tgagccccgg
    11581 ctgtaacgtt ttggtatagc gaccaaaacg ttctaccgtc cattgatagc cctgcggcac
    11641 gattttgacg cccgcgccga caatgaccag cgcgacaaaa atgagaatcg ggataaagat
    11701 aagcatcgga aaaacctcct gttgtaccgt ccataatcag caaaattgct gcttgattaa
    11761 acaaattata cctgattact gaaagagagt tcccccttat tcctgcgaag gataaactgt
    11821 ttttagtaaa aatcagaaaa agggaacagc gatgcaggaa aatagtcctt tgcttcagct
    11881 acaaaacgta ggatatctgg cgggtgatgc gaagattctt aataacatca atttttcgct
    11941 gcgtgctggc gaatttaagt taattaccgg tccttctggt tgtggcaaaa gtacgctgct
    12001 aaaaatagtt gcttcattga tcagcccaac cagcggaacg ttactgtttg aaggtgagga
    12061 tgtcagcaca ctaaagccag aaatctaccg ccaacaagtc tcttactgcg cccagacacc
    12121 gacgctgttt ggcgatacgg tatacgataa tctgatcttt ccttggcaga tccgtaaccg
    12181 gcagcctgac ccagccattt ttctcgattt tctcgaacgc ttcgccttgc cggacagcat
    12241 tttgacgaaa aatatcgccg agctatctgg tggtgaaaaa caacgcatct cactgattcg
    12301 taacctgcaa tttatgccga aggttttatt gctggatgaa atcaccagtg cgctggatga
    12361 aagtaataaa cataacgtta atgagatgat ccatcgttat gtgcgcgagc aaaatattgc
    12421 cgtgctgtgg gtgacacacg ataaagacga aattaatcat gcggataaag tgattacact
    12481 gcaaccgcat gccggagaaa tgcaggaagc acgctatgaa ctcgcataat attactaacg
    12541 aatcattggc actggcatta atgctggtgg tggtggcaat cttaattagc cataaagaaa
    12601 aactggcgct ggagaaagat attttctgga gcgtcgggcg cgcgataatt cagctgatta
    12661 ttgtcggcta tgtgctgaag tatattttca gcgtggatga tgccagcctg acattattga
    12721 tggtgttatt tatctgcttt aatgcggcgt ggaacgcgca aaaacgcagt aaatatattg
    12781 ctaaagcttt tatctcatcg tttattgcca ttacggttgg ggcgggaatt accctggcgg
    12841 tgctgattct ctcagggtcg attgaattta tcccgatgca ggtgatccct atcgccggga
    12901 tgattgccgg taacgccatg gtagcggtgg ggttgtgtta caacaattta gggcaacggg
    12961 tcattagcga acagcaacag attcaggaga aactgagtct tggcgcgacg ccgaagcagg
    13021 cttcagcgat attgattcgc gacagtattc gcgcggcttt aattccgacg gtcgattcag
    13081 caaaaacggt tggcttagtg agtttaccag gaatgatgtc cgggctgata tttgccggga
    13141 ttgatccggt gaaggcgatt aaatatcaga ttatggtgac ctttatgctg ctctcaaccg
    13201 ccagcttgtc gaccattatt gcctgctatt taacctatcg taagttttat aattcgcgcc
    13261 accagttggt ggtgacgcaa ttgaagaaga aatgatgcga tgccggatgc gcagcatcgc
    13321 atccggcgtt gtggttcatg tgccgaacaa cggaatatca atacaacaat gcatacagct
    13381 ggcggcgata cttcgacgcc agtgcatcac ccgtacccag cgcagcgagg atctcctgga
    13441 acgttttacg cgtctgaccg tctgcggcgg tgagatcttt acgcagatgc ccgaacagca
    13501 actccagcgc ctcttcattg cgcccaacct gatgcagttg cagcgccagt tgcgtcgcca
    13561 gtgcggcatc ttctggattc tccgccacct gctgttgcaa ctgttgaatt tccggcgtat
    13621 cagccgcctg cttcagcagt tcgatttgcg ccaccagccc ctggtagcgg gtgtcctgat
    13681 cctgcaacgg aatggttttc agcaccgctt ccgcatcttc agaacggttc agcgcaatca
    13741 gcgtttctgc cagcagcagg ccgatctccc cgttctgatt cgacaactgc caggcgtctt
    13801 tcagcaatgg cagggcatcg gtgtaattgc cttcctgcat cagttgcatc gcctgctgcg
    13861 ctttcagctc ttcttcgcgc ggcagcactt tatccagcag ggcgcggatc gcctcttccg
    13921 gttgcggccc ctggaagcca tctaccggtt gcccgttctg gaacagatac acggtcggaa
    13981 tcgcgcgcag accaaactgg gcggcaatca tctgctccgc gtcgcagtcc agcttcgcca
    14041 gaataaattg cccgttgtac tgcgccgcga ggctttccag gatcggggtt aactgcaaac
    14101 agtgctggct acgttcagac caaaaataga acagcaccgg agtggtcatc gactgttcaa
    14161 gaacctgttg caggttagat tcgttaatgt tgacaatatt ttctacggac atggagtcgc
    14221 tctctgttgt cgatattttc tttgacatgg gggcttaagc gcgcgcttca actcacccct
    14281 gcaatatttt gtccatcacg cgcccaggca gcaggcgctt gagcaccatt actgcccagg
    14341 tcaccagcgt taccggatag cgcatcttcg gcttctcgct aataaaagca tggcgcactt
    14401 tgtcgaccac cgcttccggt cccaacgtaa agcgggcggc gatgccggga ttttcgactg
    14461 gtttatcact ttgcgtctgg ttgacgttgt cggtgaagcg agtacgaatg ggaccgggtt
    14521 cgatcaggct gactttaatt ccgctgtggc gtagctccat gcgcagcgcg tctgaccacg
    14581 cctccagcgc atatttactg gccgcgtaag cgccacgacc cggcgtggag attaatccca
    14641 tcaccgatga tgtcatcaca atacgccctt caccgtgcgg taacatcgcg ggtaacaggc
    14701 gcatggtgag ctggtgtgcg ccgaaaaagt tggcggaaaa ctgctgttcc atctgcgcac
    14761 ggctgatggt ggaaaggggg ccatacatgc cgaatccggc attgttaaag atcccataca
    14821 gacaattatc ggtcagggcg atcacctcgt ctgctgcgcg atcaacactt tctggtgaat
    14881 ccagatcgat caacacgccg gtaaatccca tgttgttcat acgctcaaca tcatccggtt
    14941 ttcggcaacc tgccagcaca tgaaaaccct ggcgttttaa ttcgagcgcg ctttccaggc
    15001 caattccact ggaacatccg gtaattaaga ccgatttttg cataacttta cctgtcagga
    15061 tctccgttgc tttatgagtc atgatttact aaaggctgca actgcttcgc catccagtcg
    15121 gcaataaacg gctgggcgtc gcggttggga tgaataccgt catcctgcat ccattgtggc
    15181 ttgaggtaga cctcttccat aaaaaagggc agcagcggaa catcaaactc tttggcgagt
    15241 ttggggtaaa tggcgctaaa ggcttcatta taacggcgac catagtttgc aggcagacgt
    15301 atttgcatta acaatggttc agcgttggcg gctttgacat cctgcaaaat ctggcgcagc
    15361 gtttgctcgg tttgctgtgg ctgaaaacca cgcaaaccgt cattgccgcc cagttcaacc
    15421 agcacccagc gcggctgatg ctgtttcagc agagccggaa ggcgcgccag tccttgttgc
    15481 gaggtgtcgc cgctgatgct ggcattaact accgacgttt tactctgcca cttatcattc
    15541 aacaaggcag gccaggccgc gctggcagac attcgatacc cggcgctcag gctatcaccc
    15601 agaatcaata acgtgtccgc tgcggcggca cggaaggtta acaggaccag gaacaggaag
    15661 ggcaaatgcc agcggaaaac attgttgaag ttcatcatct taagaagtcc gtcggtcagg
    15721 gggagcatga actctccatc ctcaccggag ttgagctggt tgtcaaacgt ggcgagacca
    15781 tcgcactggt gggcgagtcg ggatcgggta agtcaacctt gctggcgatc ctcgccgggc
    15841 ttgatgacgg cagcagtggc gaagtgagtc tggtgggaca accgctacat aatatggacg
    15901 aagaagcgcg ggcaaagttg cgcgcgaagc acgtcggctt tgtttttcag tcatttatgt
    15961 taattcctac ccttaacgcg ctggaaaacg tcgagcttcc ggctctgctg cgcggtgaga
    16021 gtagcgcgga aagtcgtaac ggggcgaaag cgttgctcga acagttaggg ctgggtaaac
    16081 gtctggatca tcttccggca cagctttccg gcggtgaaca gcaacgagtg gcgctggcac
    16141 gagcctttaa tggtcgacct gatgtgctgt ttgccgacga acccaccggc aaccttgacc
    16201 gccagacggg cgataaaatt gccgacctgc tgttttccct caaccgtgaa catggcacca
    16261 cgttgattat ggtgacccac gacctgcaac tggcggcacg ctgcgaccgc tgcttacggc
    16321 tggtgaacgg gcagttgcag gaggaagcat gattgcacgt tggttctggc gcgaatggcg
    16381 ttcgccgtcg ctattaattg tctggctggc gctaagcctg gcggtggcct gcgtgctggc
    16441 gctgggcaat atcagcgatc gcatggagaa gggcttaagc cagcaaagcc gtgagtttat
    16501 ggcgggcgat cgggcgttgc gcagttcacg cgaagtgccg caagcgtggc tggaggaagc
    16561 gcaaaagcgc ggcctgaaag tcggcaagca gctgactttc gccacaatga cctttgcagg
    16621 cgacacaccg cagctggcga acgtcaaagc ggtggatgat atctacccga tgtatggcga
    16681 tctgcaaact aatccccctg gcctgaaacc gcaggcgggc agcgtattgc tggccccacg
    16741 cctgatggca ctgcttaacc tgaaaacggg cgacaccatt gacgtgggcg atgccacctt
    16801 gcggattgcc ggagaagtga ttcaggaacc ggattccggt tttaacccct tccagatggc
    16861 tccgcgtctg atgatgaatc tggcggatgt cgataaaacc ggagccgtgc agccggggag
    16921 tcgggtcacc tggcgttata aattcggcgg caacgagaac cagctcgacg gctatgagaa
    16981 atggttgtta cctcagctta aacccgaaca acgctggtac ggtctggaac aggacgaagg
    17041 cgcgctgggg cgatcgatgg aacgctcgca acagttcctg ctgctttcgg cgcttctgac
    17101 cttgctgctg gcagtggcag cggtggcggt agcgatgaat cattactgtc gcagtcgcta
    17161 cgatctggtg gcgatcctca aaacgctggg ggcagggcga acgcaactgc gtaagctaat
    17221 cgtcggtcag tggttgatgg tgctgacgct ttcagccgtt accggtgggg ccataggcct
    17281 gttgttcgaa aacgtgttga tggtgctgct caagcccgtt ctgcctgctg cactaccgcc
    17341 agccagcctc tggccgtggc tgtgggcgct tggcaccatg acggtcatct cgctgctggt
    17401 ggggctacga ccataccgct tgttgctggc aacgcagcct ttacgcgtat tacgtaatga
    17461 tgtggtagcg aacgtctggc cgctgaagtt ttatctgccg attgtcagta tggtggttgt
    17521 gctgctgctc gccggattaa tgggtggcag catgctgctt tgggcggtgc tggcgggcgc
    17581 agtagtactg gctttgctgt gcggtgtgct gggctggatg cagctgaatg tactttgccg
    17641 catgacgctg aaatcgctac ctctgcgcct ggcggttagc cgcctgttac gtcagccgtg
    17701 gtcaacgtta agccagcttt cggcattttc gctctccttt atgctgctgg cgctgttgct
    17761 ggtattgcgt tgcgatctgc tcgaccgctg gcagcagcta ccgccagaaa gcccgaacta
    17821 ctttttaatt aacatcgcca cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca
    17881 tcatatagtc ccggaatcat tttatccggt agtgcgggcg cggctgacgg cgattaacga
    17941 taagccgaca gaaggtaatg aagatgaggc gcttaatcgc gaactcaatc ttacctggca
    18001 aaatacgcgg cccgatcata atccgattgt cgccggtaac tggccgccaa aagccgatga
    18061 agtgtcgatg gaagaggggc tggcgaaacg cttaaacgtt gccctcggtg gtaccgtgac
    18121 ttttatgggc gatacccagg agttccgcgc taaagtgacc agcctgcgca aagtggactg
    18181 ggaaagtctg cggcctaatt tctattttat tttcccggaa ggggcattag acgggcaacc
    18241 gcagagctgg cttaccagtt tccgctggga gaatggcaac ggcatgttga cgcaactcaa
    18301 ccgccagttc ccgaccatta gcctgttaga tattggcgcg attttaaaac aggtcggtta
    18361 ggtgctggag caggtaagtc gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg
    18421 tatgttgctg ttgctggcac aggtgcaggt gggaatgcgc cagcgtcatc aggagctggt
    18481 ggtatggcgc acactcggtg cggggaaaaa actgctgcgc acgacattgt ggtgtgagtt
    18541 cgccatgctt gggtttgttt ccggcctggt ggccgcaatt ggtgcggaaa cggcactggc
    18601 agtgttgcag gcgaaagtgt ttgatttccc gtgggagcca gactggcgat tgtggattgt
    18661 tctgccgtgc agcggtgcgc tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact
    18721 ggttaagggt aaggcgctgt tcaggcagtt tgcggggtga tgagagtaaa taaggggcgt
    18781 tctggttgaa tggaacgcct tgtttaaatt aattctacaa tatgtttcat tgatgatgtt
    18841 attgaattgg tgatttccta tctttctatt gctgatatta atctgaattg gtggagttta
    18901 ataattttaa atatagccct acaaaaataa ctcagactaa taaaatcata aatcatatgc
    18961 gttgaatgga tattatccat atagtgaatt tgttgatgat gaattcatct gtgctaaaaa
    19021 ttttagttta ataaaatatt gaaagtgacc tgtaataaca gttattgttg attgagaaca
    19081 aataagttta tgtgaaaaat atataaatac attagctggt catgtgtgtc attttatttt
    19141 tttgttgcta acacagggat atgaacaata actaaaaggg cactttatat gagcggaaaa
    19201 ccagcggcgc gtcagggaga tatgactcag tatggcggtc ccattgtcca gggttcggca
    19261 ggtgtaagaa ttggcgcgcc aactggcgtg gcgtgctcgg tgtgtccggg cgggatgacc
    19321 tcgggcaacc cggtgaaccc gctgctcggt gcaaaagtac agcccggcga gacggacctt
    19381 gcgctgcccg acccgctgcc gttcattctc tcccgcacct acagcagcta ccggacgaag
    19441 acgcctgcgc cagtgggaac tttttggccc cggctggaaa gcgccttctg atatccgctt
    19501 acagatacgc gatgatgcac tggtactcaa cgacaacggc gggcggagca ttcactttga
    19561 gccgctgctg ccggggaagg ctgtgtacag ccgcagtgag tcaatgtggc tggtgcgtgg
    19621 cggtaaggca gcgcagccgg acggccacac gctggcacga ctgtgggcgt cgctgccgcc
    19681 ggatatccgg ttaagcccgc atctttacct ggcgaccaac agcgcacagg ggccgtggtg
    19741 gatactgggg tgatctgagc gggtgccggg tgctgaggac gtactgccag cgccgctgcc
    19801 gccgtaccgg gtgcttaccg ggatggcgga ccgcttcggg cggacgctga cgtaccggcg
    19861 tgaggccgca ggcgacctgg ccggggaaat caccggcgtg acggacggtg ccgggcggga
    19921 gttccgtctg gtgctgacca cacaggcgca gcaggcggaa gaggcccggc agcaggcggt
    19981 cgtcgccgga gcaaaacgac cggacattcc tgattcactg ccggagagca cagagtacgg
    20041 ccccgacagg ggtatccgcc tttcggcggt gtggctgatg cacgacccgg catacccgga
    20101 gagcctgccc ggtgcgccac tggcgcggta cacgtatacg gaagccggtg aactgctggc
    20161 ggtatatgac cgcagcaata cgcaggtgcg cgctttcacg tatgacgcgc agcacccggg
    20221 ccggatggtg gcgcaccgtt acgcgggaag gccggagatg cgctaccgct acgacgatac
    20281 ggggcgggtg gtggagcaac tgaacccggc agggttaagc taccgctatc tttatgagca
    20341 ggaccgcatc accgtcaccg acagcctgaa ccggcgtgag gtgctgcata cagaaggcgg
    20401 ggccgggctg aaacgggtgg tgaaaaaaga actggcggac ggcagcgtca cgcgcagcgg
    20461 gtatgacgcg gcaggaaggc tcacggcgca gacggacgcg gcgggacggc ggacagagta
    20521 cggtctgaat gtggtgtccg gcgatatcac ggacatcacc acaccggacg ggcgggagac
    20581 gaaattttac tataacgacg ggaaccagct gacggcggtg gtgtacccgg acgggctgga
    20641 gagcagccgg gaatatgatg aaccgggcag gctggtatcg gagacatcgc gcagcgggga
    20701 gacagtacgc taccgctacg atgacgcgca cagtgagtta ccggcgacga caacggatgc
    20761 gacgggcagc acccggcaga tgacggcggt tcaccgcgag gaaggtatca gcctttaccg
    20821 ccactatgac aaccgtggcc ggttaacctc ggtgaaagac gcacagggcc gtgaaacgca
    20881 gtatgagtac aacgccgcag gcgacctgac tgccgttatc accccggacg gcaaccggag
    20941 cgagacacag tacgatgcgt ggggaaaggc ggtcagcacc acgcagggcg ggctgacgcg
    21001 cagtatggag tacgatgctg ccggacgtgt catcagtctg actaacgaga acggcagtca
    21061 cagcgacttc agctacgatg cgctggaccg gctggtacag cagggcggct ttgacgggcg
    21121 gacgcaacgt tatcattatg acctgaccgg aaaactcacg cagagtgagg atgaggggct
    21181 tgtcaccctc tggcactacg atgcgtcgga ccgcatcacg caccggacgg tgaacggcgc
    21241 cccggcagag cagtggcagt atgacggcca cggctggctg agagaaatca gccacctgag
    21301 cgaaggccac cgtgtcgctg tccactacgg ctacgacgat aagggccgcc tgacgggcga
    21361 acggcagacg gtggagaacc cggagacggg ggaactgctg tggcagcatg agacgaaaca
    21421 cgcctacaac gaacaggggc tggcaaaccg cgtcacgccg gacagcctgc cgccggtgga
    21481 gtggctgacc tacggcagcg gttacctggc gggcatgaag ctgggcggga cgccgctggt
    21541 cgagtatacg cgggacaggc tgcaccgtga gacggtgcgc agcttcggca gcatggcagg
    21601 cagtaatgcc gcatatgaac tgaccagcac atacaccccc gcaggccagt tacagagcca
    21661 gcacctgaac agcctggtgt atgaccgtga ctacgggtgg aatgacaacg gcgacctggt
    21721 gcgcatcagc ggtccgcgac agacgcggga atacggctac agcgccacgg gcaggctgga
    21781 gagcgtgcgc accctcgcac cggacctgga catccgcatc ccgtatgcga ccgacccggc
    21841 gggaaaccgg ctgccggacc cggagctaca cccggacagc acgctcacgg cgtggccgga
    21901 taaccgcatc gcagaggatg cgcactatgt ttaccactat gatgaatacg gcagactgac
    21961 ggagaagacg gaccgcatcc cggcgggtgt gatacggacg gacgacgagc ggacccacca
    22021 ctaccactac gacagccagc accgcctggt gttccacacg cggatacagc atggcgagcc
    22081 actggtcgag agccgctacc tctacgaccc gctgagccgc cggacgggga aacgggtgtg
    22141 gcgtcgcgag cgtgacctga cggggtggat gtcgctgtcg cgtaaaccgg aggtgacgtg
    22201 gtacggatgg gacggcgacc ggctgacgac ggtacagacc gacaccacgc gtatccagac
    22261 ggtataccag ccggggagct tcgcgccgct catccgcatc gaaacagaca acggtgaacg
    22321 ggagaaagcg cagtgccgca gcctggcgga gaagttacag caggaaggga gcgaagacgg
    22381 tcacggtgtg gtgttcccgg ctgaactggt ggggctgctg gacaggctgg agggggaaat
    22441 ccgggcaaac tgcgtgagca gtgaaagccg ccagtggctt gcgcagtgcg ggctgacggt
    22501 ggagcggctg gcggcacaga tagagccggt ttatttgcct gagcgaaaaa tacatcttta
    22561 tcactgtgac caccggggcc tgccgctggc gcttatcagt gaagacggca atacggcgtg
    22621 gagcgcggaa tatgatgaat ggggcaacca gcttaatgag gagaacccgc atcacctgca
    22681 ccagccgtac cggctgccgg ggcagcagta tgataaggag tcggggctgt actacaaccg
    22741 gaaccggtac tacgatccgt tgcaggggcg gtatatcact caggacccga tagggctgga
    22801 ggggggatgg agtctgtatg cgtatccgct gaatccggtg aatggtattg atccattagg
    22861 gttaagtccc gcagatgtag cgctaataag aagaaaagat caactaaacc atcaaag
//
LOCUS       DAJTNN010000054        22844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTNN010000054 DAJTNN010000000
VERSION     DAJTNN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.054"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            29..958
                     /gene="aaeR"
                     /locus_tag="OO150_001834"
     CDS             29..958
                     /gene="aaeR"
                     /locus_tag="OO150_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5516004.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1086..2531)
                     /gene="tldD"
                     /locus_tag="OO150_001835"
     CDS             complement(1086..2531)
                     /gene="tldD"
                     /locus_tag="OO150_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5516005.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2687..6487)
                     /gene="yhdP"
                     /locus_tag="OO150_001836"
     CDS             complement(2687..6487)
                     /gene="yhdP"
                     /locus_tag="OO150_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5516006.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFHLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6555..8024)
                     /gene="rng"
                     /locus_tag="OO150_001837"
     CDS             complement(6555..8024)
                     /gene="rng"
                     /locus_tag="OO150_001837"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5516007.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8014..8607)
                     /gene="yhdE"
                     /locus_tag="OO150_001838"
     CDS             complement(8014..8607)
                     /gene="yhdE"
                     /locus_tag="OO150_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5516008.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAHKKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8616..9104)
                     /gene="mreD"
                     /locus_tag="OO150_001839"
     CDS             complement(8616..9104)
                     /gene="mreD"
                     /locus_tag="OO150_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5516009.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9104..10189)
                     /gene="mreC"
                     /locus_tag="OO150_001840"
     CDS             complement(9104..10189)
                     /gene="mreC"
                     /locus_tag="OO150_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5516010.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPTTGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10255..11298)
                     /gene="mreB"
                     /locus_tag="OO150_001841"
     CDS             complement(10255..11298)
                     /gene="mreB"
                     /locus_tag="OO150_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5516011.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11603..13543)
                     /gene="csrD"
                     /locus_tag="OO150_001842"
     CDS             complement(11603..13543)
                     /gene="csrD"
                     /locus_tag="OO150_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5516012.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKYPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13695..14669
                     /locus_tag="OO150_001843"
     CDS             13695..14669
                     /locus_tag="OO150_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5516013.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVMVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15647..16117
                     /gene="accB"
                     /locus_tag="OO150_001844"
                     /gene_synonym="fabE"
     CDS             15647..16117
                     /gene="accB"
                     /locus_tag="OO150_001844"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5516014.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16128..17477
                     /gene="accC"
                     /locus_tag="OO150_001845"
     CDS             16128..17477
                     /gene="accC"
                     /locus_tag="OO150_001845"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5516015.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17569..18616)
                     /locus_tag="OO150_001846"
                     /pseudo
     CDS             complement(17569..18616)
                     /locus_tag="OO150_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(18613..19575)
                     /locus_tag="OO150_001847"
     CDS             complement(18613..19575)
                     /locus_tag="OO150_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5516016.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDVHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            complement(19597..20586)
                     /locus_tag="OO150_001848"
                     /pseudo
     CDS             complement(19597..20586)
                     /locus_tag="OO150_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(20587..22086)
                     /locus_tag="OO150_001849"
     CDS             complement(20587..22086)
                     /locus_tag="OO150_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5516017.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAERGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRITNPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(22147..>22844)
                     /locus_tag="OO150_001850"
     CDS             complement(22147..>22844)
                     /locus_tag="OO150_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5516018.1"
                     /translation="QDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIKADKAGIP
                     VITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQSVVARVD
                     GFKKGLSDTPDIKIVAEQPSITRAEALTTAQNIMQANANLNGLFGFGDDAALAAVIAA
                     KSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDHLNGKPVE
                     KMIPVQPGVYTGK"
BASE COUNT         5662 a         6190 c         5455 g         5537 t
ORIGIN      
        1 ctaaatcact tttaagtcag agtgaataat ggaacgacta aaacgcatgt cggtgtttgc
       61 caaagtagtt gaatttggct cttttaccgc cgccgccaga cagctacaga tgagcgtttc
      121 gtccatcagt cagacggtgt caaaactgga agatgagttg caggtaaagc tgttaaaccg
      181 tagcacacgc agcattggcc tgaccgaagc cggtagaatt tactaccagg gctgccgtcg
      241 tatgcttcat gaagtgcagg atgttcatga gcaactgtat gctttcaata acacccccat
      301 cgggacgcta cgcattggct gttcttcaac tatggcacaa aatgttctcg ccgggctgac
      361 agccaaaatg ctgaaagaat acccaggttt gagcgtcaat ctggttaccg gaattccagc
      421 ccccgacctg attgccgacg gtctggatgt ggtgatccgc gtcggcgcgt tgcaggattc
      481 cagcctgttt tcccgccgtc ttggcgcgat gccaatggtg gtgtgcgccg cgaaaagcta
      541 tctcacacaa tacggcatac cggaaaaacc cgccgattta agtagccatt catggcttga
      601 atacagcgtg cggcccgaca atgaatttga actgattgca ccggaaggga tctcgactcg
      661 cctgatcccg caagggagat ttgtgactaa tgatccgatg acgctggtgc gctggctaac
      721 ggcgggtgcc gggatcgcct acgtgccgct gatgtgggtg atcaacgaga tcaatcgtgg
      781 ggagctggag atcctgctgc cgcgttatca gtcagatcca cgcccggttt atgcgttata
      841 taccgaaaaa gataagctgc cgctgaaggt acaggtcgtg atcaactcgc tgacggatta
      901 ttttgttgag gtcggtaaat tgtttcagga gatgcacggg cgcgggaaag agaagtaatt
      961 catattgtac tgttacgttc tacaaacctg tgccaacggg ttcccctcac cctaacccca
     1021 gaggagcgag gggaccgttc gtgcacgtag aaagattaat tatccttctg aaaataatga
     1081 aattattacg cagtaccgcc aacagtcagg ttatcaactt tcagcgttgg ctggcccacg
     1141 ccaaccggca aactttgccc ttctttaccg cagacaccca cgccgttatc cagtttcagg
     1201 tcgttgccaa ccatcgaaat ctgctgcatg gtttcgatac cggaaccaat caacgttgcg
     1261 cctctcaccg gcttcgttac tttaccgttt tcaatcagat atgcttctga agtggagaaa
     1321 acgaatttgc cggaggtgat atccacctga ccgccaccaa agttcggtgc atagataccg
     1381 tactcaacgg attcaataat ttcctgcggg gtcgatttac ccggcagcat ataggtgttg
     1441 gtcatacgcg gcatgggcag atgggcgtag gattcacggc gaccgttgcc agtcggcgtc
     1501 atccccatca aacgcgcgtt gagtttatcc tgcatgtagc ctttcagaat gccgttctca
     1561 atcagcacgt tgtactggcc tggcgtacct tcgtcatcaa tcgccaccga accacggcga
     1621 tcgaccatcg tgccgtcatc aaccacggta cacagttctg aagccaccag ctccccgacc
     1681 tgcccactaa atactgaagt gccgcggcgg ttgaagtcgc cttccagacc gtgacctacc
     1741 gcttcatgca acagcacgcc cggccaacct gcgccaagta ctaccggcat ggtgcccgct
     1801 ggtgcagcaa cggcagaaag attgaccagc gccatacgca ctgcttcttt tgcccatgca
     1861 tccgcacgga cttcgccgtc aagatcggca aggaagaact cataaccaaa acgaccgcca
     1921 ccgccactgg caccgcgttc gcgtttgcca tcttcttcga ccagaacgct cacggaaaga
     1981 cgcaccagcg gacggacatc cgccgctagc gtgccgtcgg tggccgcaac cagaattaat
     2041 tcatagacac cactgaggct ggcagtcact tcctgtacgc gcttgtccgc ttcgcgagca
     2101 accttatcga cgcgacgcag gatatccagc ttctcttcac ggctcatgct ttgcagcgga
     2161 tctaccgagg tatacaacgg gctatgccct accgcgccca gcgtctgtac tttgccatca
     2221 ccactatcac ggacgatggt gcgcgccgct tgcgcactct gttccagcgc cagcaggctg
     2281 atttggtcag cgtaagcaaa tccggttttt tcaccgctga ttgcacgcac gccaacgccc
     2341 tgatcgatgt tgtaagaacc atctttaata atgcggtctt ctaaaaccca ggattcgtga
     2401 tagctcgact gaaaatagag atcgccataa tcaaggcgac gttcggccag ttgaccgagg
     2461 atcgcgaaca agtcctgatg tttcaggccg ttcgccgcta gcaattgttc acttactagg
     2521 ttaagactca tcgtttttgc tactcgttag ttactgcagt agaggatttt ttacggctgc
     2581 cggatgcggc gtgaacgccc tatccgacct acggttatgt tctggctcgt tggcctttgg
     2641 caacgattat catatgagat tggggcaatt acgcgccctc gtcaaatcat tgcgcttttt
     2701 ctttacgcgg ttggcgcaac acttcgttga tttgcggatc gtccagcgga cccgaaatgt
     2761 gatagcgcaa aatggagact ttgctccaca gcggccccag cactttactg gctgcaaata
     2821 ctgccgcgcc aacaatgggg ttaaccgcaa aagccgcagc cacgcccacc gtcgcagaaa
     2881 tctctggtgc gacaaccgct tccatattca ggtcgcgacg taccagattt accgaccctt
     2941 tcatggcgat atccgcctcc aggccatcca ccagcgtgtc gtcggtgtgc ataacgccgt
     3001 ctttaatcca cgcggtgctg cgaatggagt caaaatagaa cccttcgcca aaagtgtctc
     3061 tgaaatcaaa acgcagctta cgcatcaggg catcaacgct caataagcgc agcaattgcc
     3121 cggcatgtcc ggtattgatt tcggtaattt cgcctttacc cagttgagta tgaatgatgc
     3181 cattcaacgt cgcctcatct ggttgccagg gtgctttgcg ccagtgtaaa tcgtaatcca
     3241 cattaaatga cgactggcgt atgggcgtcg tgacaccaaa aaattctgcg gcggcatcaa
     3301 ttttctgccc gcgcagtttt cctttcagcg aggtacgttc attccccgga ttattaaccc
     3361 attcaccatc ggcagtaagc cgcgagaaac cagtatcaat cagtccattg gtcagcgtta
     3421 acgtatcgcc agaaatggtg agatcactgt caatacgacc gaatttttgc ccccagaacc
     3481 agcactctgt gcatcgtatt tgtgcgtccg gccagccacg gaagttaatg cgctccgttg
     3541 tcgggaaagg tgatgacggt gttgaatcac cacgagtttt cgccacgctc gggttgtaat
     3601 aaagatattt gatattcgcc agccacggcg cgttattacg catcgccagc gtggcattga
     3661 tttcacgccc ctgcgcctca accagggtgc catttgccgt cggttgcgaa acaatactca
     3721 ggttattcca ttgctgattt cccagtgaca acataggcgt acgtaacgtt atgtgttgtg
     3781 ggaaactcgc tgcaccaccg acactctccg cagcgccttt ctggaacagg gccagccact
     3841 cggcaccatt catcggcggc atattgagtt caacgccact ttgttccggc aacggcggga
     3901 gcgttttact gtctgccgcc caaatagcac gatcgagcgt cagcttttga ccaagcaacc
     3961 agcggctatt gaaatggtta tccgcaccag cctgtccggt taattcaaag ctgctgagat
     4021 tgccatcaac cttaacgttt accggcagtg gttcacccgc aggtttggct aacggtgaag
     4081 gtaagtgact gctcacatta ttcagatcgc cgttcagctc tacgttatag gtcgcgccag
     4141 catgataagg cagatcaatg cccactttac catcccacgc cacgctgcca ctcaatgctt
     4201 cgttcaccgc tgcaggcaga acgccggttt tcgccggttg ccagttaccg ttgaggttta
     4261 ccgcaacctg gtaggctttt gccccttctt tggtggaaaa atccacgttc aacggctgat
     4321 taaaccagct tgctgtcagt ggttcacttt gcagatcgcc attgataaag ctgaatttac
     4381 cgctcaaatt tttcagggtg ctgtcgagta gtttgataaa cagactgtta ttacgcagcg
     4441 tcacttcacc tttcgcggtt accagttcgc cgttcagcgg gatatcaaga tgtaagcgag
     4501 cattcacatc gccgtcgagc tggagttctt gcagggtcgc acccagagaa tctttcagcg
     4561 gtgtctcatc aaagtaaggg ccaacggctt tacccggacc tttaatgtca gcgtcaatca
     4621 gcagtttttc tttcgagtag tcagggatca ctgcggtgag attactcgcg cgcacgccgc
     4681 ccagattaac gtcatcggtt ttcatccata aaccgtcgtt aataaagtcc agttcaatat
     4741 caaggttagt taatgcaggc cagtccggct ggaaggcaaa cttcgcgtta cgcagcggca
     4801 ccagcacttc aaactgacct tcgttgtgtt tataggggaa gagttgcgga ttgccaccat
     4861 aaaccagcgt cgcgttatcc gcttcaccgc cctgaatcgc gccacttaag taatcaacta
     4921 ggtctttacc catcaagttt tccgggaaat agcgccaggc ttgtgaacca tcatcggtac
     4981 tgatgccagc cagaataccc agccagggtt catcgttagc aggttgcagg taacgaaaac
     5041 cgccgcgcgc atggacagct ttggctttaa cgtcaatatt acgcccatcc aggtggaaac
     5101 ctttgtcatt gttcagccag cttatagttg cctggccgtc ggcgatttcc agtggcgcac
     5161 ggaataccgt ttcgtaaggc atctttgcct gcttcatcga cgcggtaagc aaaccatttt
     5221 caacgctgcc ggaaagcgtc ccggagaagt gttccgcacc cggtaataat ttccattgct
     5281 tccaggccag atcgctccac gatgcctgaa aacgggtctt gtctgccgcc tgaagcggga
     5341 tatccagcgc cagagtgtta atcttgccgc tcggttgtgt ggagcgccaa acatcaccca
     5401 gtgcaggtga aagtttcgcg gccagcgggc gtatgccctc caggcctgcc agctccagat
     5461 tactggcgcg aatccggagt tcgtcactgc gtttattgtc tttgccgcca acgtcctgtt
     5521 ccggtatcca ggccagcgtc aatgctccgc tcggccaggg tttgccgtcc atcgtgatcc
     5581 gtgtatcggg aatagagaac tgccagcccg gattttcacg cgtaatatgc gcggtcagat
     5641 tatccaccga cagcgtatgc gtttgcttct cgcccaacca gctggcaccg ccctgtttca
     5701 gccagacgtc accgccggtt acatcgcctt tgtcgatcgt catccagcct tcaagggaga
     5761 actgtgccgt ttccagagca atattgtcct gcatccattt accgagccac ggcttcaggt
     5821 cgatgtcatc cgcctgtagc cagacgcgac cattgcttaa caacccctca tcatcgcgca
     5881 aatccatgcg cacctgcatc acgccgtgct gtccggtaag gctggagagg cttaccaggc
     5941 cttccgcacg gtgtcgacgt ggatcgttca gccaggtgag ttgtgggatc gccagctcgg
     6001 cgcgctgacc ggatggcgtc agaaaactga cttcactgtc gcggagatcg aaatggtcaa
     6061 attgacgaag aaacagatcg ctgatgtgac tggcttccag gctatcatta ccaccaccgc
     6121 tggtgatagg agtgttggtg cgaaagcgca gctgccagaa agtgaggtcg cgaaactgcc
     6181 agcgcatatg taacaagctc tgccagacat ccagcgccag agtaacgcgt ttaaccgaaa
     6241 attcgccgcc atcttttagt tctgcacgga tgtcgtgtgc ttcaagcgtc gggccaaaat
     6301 tctgccagct ggctgagagc tgactggctt ctaccggcat gccagtcgcg gattctattt
     6361 tgttgaggat ttccggacgc caggcgtcaa gatgcggtaa agcaatacgc aggccgctaa
     6421 ccagcagggc tgcgatcaca acgagcgcgg ctccagtaag cagtaaaatc cccggcaatc
     6481 gcctcacccg tcactccttg tctgctaaaa atgtgactca aaaacccttt gccggatggc
     6541 ggcccagcat ctgtttacat cattacgacg tcaaactgct cctggttata gagcggttca
     6601 atttgtactt taacctgttt gccaacgaaa atttccactt ccgccagcga gtgtgactct
     6661 tcgcctttca aggcttcagc tactgccgga gaagcataga ccaggaaacg gtcggagtcg
     6721 taagcatggt ggacacgaac aatctcgcgc atgatttcat agcataccgt ttccacggtt
     6781 ttcaccgttc cgcgaccgtg gcaggttggg cattcgttac acagtacgtg ctcaatgctt
     6841 tcgcgggtgc gtttacgcgt catctccacc agccccagcg ccgaaaaacc attaacgctg
     6901 gttttcaccc ggtctttgct caacgcctgc tccagcgagt gcagcacgcg gcggcggtga
     6961 tcttcattat tcatatcgat gaaatcaata atgataatcc cacccagatt acgcaaccgt
     7021 aactggcgag cgatagcctg cgtcgcttca atattggtat tgaaaatggt gtcgtccaga
     7081 ttgcgatgac cgacaaacgc tccggtattg atgtccacgg tggtcatcgc ttcggtctgg
     7141 tcgataatca gataaccacc ggatttcagt tctactttgc gttccagcgc tcgctggatt
     7201 tcgttttcga catcaaagag atcgaaaatc ggctggcgtc cggtgtaatg ctccagcttg
     7261 ctggtcatct cgggaatgta ctccgaggta aactccagca atgcttcgta agtcaggcgt
     7321 gagtcaacgc gaatgcggtc cagttcggcg tcggcgaaat cacgcagaac acgctgcgcc
     7381 agcgccagtt cgccgtacag ctgataacgg gtctgcgggc gttttttacg ctccataact
     7441 ttggtccaga cgcgtttcag ataagcggca tcggaggcca gttcagcctc gccaacccct
     7501 tccgctgcgg tacggatgat aaacccgccc tgctcgtcgc aatactctgc gaccactttt
     7561 ttcaggcgtt cacgttctga ttcgctttca atacgttggg aaaccccaac gtgagaagcc
     7621 cctggcataa acaccagata gcgagaaggc agcgtgatat cggtggtcag gcgcgcacct
     7681 ttagtgccaa gcggatcttt caccacctgc accatcagat cttgcccctg acgaaccagt
     7741 tccgagatgt cgcgcaccgt gaattgcttt tgttcttcac ccgccacaca ttcggtgtgc
     7801 ggcatgatat cggatgcatg aagaaacgcg gctttatcca gcccaatatc tacaaaagcc
     7861 gcctgcatac ccggaagtac acgacttaca cgacccttgt agatattgcc tactattccg
     7921 cgtcgcgcct cacgttcaat atgaatttcc tgcagaatac cgccatcaat atacgccact
     7981 cgcgtttccg aaggcgttac gtttactaac aattcagccg tcatgtttat cccttttctc
     8041 acgcagtgcg ttaaaattac ttaataattc atacgtttca accagcggta agccgactac
     8101 ggcgtgatag ctgccattta tcttcctgac aaaacagcca cccagcccct gaataccgta
     8161 tgcacctgct ttatctaacg gttcaccgct ggcgacatag cccgcgatgt cttcgtctgt
     8221 taacgttctg aaagtcacat cggtgaccac caggcaatcg agaatgtgct ggctgtcggc
     8281 caacaccact gccgtcatca cctgatgggt ctgacccgat aatttgcgca acatctgcgc
     8341 cgcatgctct gcgtcgcgcg gtttctccag cacttctcca ttcaggataa cgatagtatc
     8401 cgcacccagc accgggagat cctgcgccgt ttgcgcgaca cccgcctgtg ctttcttgtg
     8461 tgccagacgc acaacatact gctgcgcgct ctcctgcggc tgacgctgct cctcaatgcc
     8521 cgtaacaata cgttcaaagg tcacgccaag ttgcgcaagt aactcctgac gacgcggaga
     8581 accggaagct aaatacagag aagtcataga aacctttatt gcactgcaaa ctgctgacgg
     8641 actttacgca tcagcaagaa aatccacggc cagagcaccc cattgactac actactccag
     8701 aacacttccg gtctgaaaga gacgttaatc actaaaaact ctgcccagaa aacaataata
     8761 tccaccacca gcgaaagcaa catgacgact agcgcctgct gccataatgc gaggttgcgg
     8821 aaaagctggt atttcagcgc caccaggtaa gcaatgatgc tcatcgccaa tacgcgtacg
     8881 ccaagcgtcg agccgctgat cagatccagt atggcaccca tcacaaaacc tgtgcccaca
     8941 tttacgcgat ggggcaaggc caggatccaa tacaacaaga taagtaacac ccagtttggc
     9001 cggaaaacaa tcaggttatc cggccagggc atgatttgca gcaacagcgc aatgaggaaa
     9061 gagagccaga ttacccaacg tccctggcta cgatagctcg ccactattgc cctcccggcg
     9121 cacgcgcagg cggttgagca ccactttgcg gcggcgtagc cctttgtgga gagcgattag
     9181 cagcaggcgc agtagctgca tttcctgtcg tcggttgctg cggagtcggc tgagcgatcc
     9241 ccgttgtcgg ttcaggtaac tttggcccca tcgcgtctgg cgaaggcaat acctgcggca
     9301 tcatctgcat cagacgttca ttagcaacac gatgcacctc ttccggcgtc atcgggttag
     9361 cgccgttacg atccgcgccc cacagcagca gcagataacg caaacgttgc agtcctgcag
     9421 tcggacgcgc ctgaatcaca gtataagcgc gctgggtatc gagttttacg gaagagacaa
     9481 ccgcgaccgg atagccttcc gggaaacgac cgcccagacc ggaggtcacc agcacatcac
     9541 caacacgaat atccgtattc gctggcagat gctcaagctg caaatcatct gtacaaccgt
     9601 taccggctgc aattacgcgg atatcgttgc gcagcacctg gattggcagc gcgtgggtcg
     9661 catcacaaat cagcagcacg cgactggtca gtttagcgac ggccaccacc tgaccaacaa
     9721 cacctttgtc gctgatgacc ggctggcctt cataaacgcc attaacgcta cctttatcga
     9781 taacaacttg atcgctataa ggatcgttaa ccgtggagat aacctgagtc accattttct
     9841 gctcatcctg acgcagcggg gaacccagca gctcgcgcag acgcgcgttc tcctgtttgt
     9901 attgtccaag catcagcagt tcactgtttt tcagcaacag ttcctgacgt aacgcccggt
     9961 tttcaagttc tagttggtca cgcgaggcca gcgtctgcga tacgccatcc agcaattcac
    10021 gaggagcatt ggaaacaaag tagaaaggac tgacggcggt atccatataa gtacggattt
    10081 gactgaacgt ccccaggcgg ctgtcggcaa taataatgcc gagcgccacc agcaccgcca
    10141 gaataaggcg aatctgtagc gacgggccac ggctaaaaat tggcttcata agttatgcgt
    10201 attctcgtat cagactaggc agggtaaaca gacatttccc ctgcctgcat ccgattactc
    10261 ttcgctgaac aggtcgccgc cgtgcatgtc gatcatttcc agcgctttgc cgccaccgcg
    10321 cgccacacag gtcagcgggt cttcagcaac aacgactgga atgccggttt cttccattaa
    10381 caaacggtca aggttacgca gcagtgcgcc accaccggtg agcaccatgc cgcgctcgga
    10441 gatgtcggaa gccagttccg gcgggcactg ttccagtgca accattaccg cgctcacaat
    10501 accggtcagc ggttcctgca gtgcttcgag gatttcgttg gagttcaggg taaaaccgcg
    10561 tggaacacct tctgccaggt tacggccacg aacttcgatt tcacggactt catcgcccgg
    10621 gtaagccgaa ccgatttcgt gcttgatacg ttctgcggtg gcttcaccga tcagagaacc
    10681 gtaattacga cgcacatagt tgatgatagc ttcgtcgaaa cgatcaccac caatgcgcac
    10741 ggaagaggag taaaccacgc cgttcaagga gataacagca acttcagtgg taccaccacc
    10801 gatatcaacc accatagaac cggtcgcttc agaaaccggc aggccagcac caattgcggc
    10861 agccatcggt tcttcaatca ggaagacttc acgggcacca gcgccctgcg cggattcacg
    10921 aattgcgcgg cgttcaacct gggtcgcgcc aaccggtaca caaaccagaa cgcgcgggct
    10981 tggacgcata aagttgttgc tgtgcacttg tttgatgaag tgctggagca ttttttcagt
    11041 cacgaagaag tcggcgataa cgccgtcttt cattgggcga atggcagcaa tattgcccgg
    11101 cgtacggccc agcatctgct tcgcgtcatg acctactgca gctacgcttt tcggtgaacc
    11161 ggcacgatcc tgacgaatgg ccaccacgga aggctcattc aatacgatgc cttgtccttt
    11221 tacataaatg agggtattcg cagtacccag gtcaatggac aagtcattgg aaaacatgcc
    11281 acgaaatttt ttcaacatac taagggataa tcctgaaagc tggggcggaa aagaaaatcc
    11341 gcttacttta ccaaccacac gcagcagcga caaggcgcaa aaatcatctg ctacggtgaa
    11401 aattagtgca gttcgtttcc tttgttacaa atctctgcct gagtccagaa aggcttaatg
    11461 catcagcagc attcctcgcc tgtttgcaac cgcgtaaggt cattcatctg catatgtgct
    11521 gcaacaatct ggcgagcaga caagcacact cccatgagac gcagcgcgcg ttattctacg
    11581 tgaaaacgga ttaaacggca ggttaaaccg agtatctttg tgaatatttt ttcacgttag
    11641 tatcaagtgg ctgtgaggac gcgaaaaaat ccccttgccc gcctgtaaca ccgcgctgaa
    11701 tcagggtctg ccactcgctt cgcgaacgca caccggtggc gtaaacctgg gtgctggtcc
    11761 cggagcaggc ttccaccagg ctttgaacca gcagctggtt ctccgttcgc ttctcaatgt
    11821 ttctgaccag ccccggatgg agcttgagta actcaacatt aagttctttg atccaactgg
    11881 tgcttaccag cgtcaaacca gcctggttga cggctacccg tacccctaaa gcattcacta
    11941 aacgaataac aggttgtaaa cgactgatat gttgacctac atcggcctct gcaagttcaa
    12001 taattatgcg ttttcgttgt gatttttcac attgcattaa cgtatcgcgc agccaacgct
    12061 gaaaacgcgg gcgaatcagc gactcaacgg taacctgaat cgccagattt tcctctggcc
    12121 agtaacgcaa tagtggaata agacggctga tttgcagacg gtcatactct tccgataagc
    12181 caaactgcaa gaccatcggc atatactccg ccgagctaac ctcttcatta ccatcgaaga
    12241 tgcggcacat gagttcgcga tgatgaacct gaccttcgcg agtaaccgcc ggtttttgat
    12301 aaaggcgcgg gccgccgcga ctgagcattt gctcgataag cgtacgccag cgaacattac
    12361 cgcgtccttt ttcaggcaac gagtcatcgt aaatagccca gctattgccg ccctgcaatc
    12421 ccgcattacg cgtggcagac tctgcatgtt ccattacctg ctcggtatcc tgaccactac
    12481 gccaggcgca gataccaatg tggatcatat cgtcgcgatc gagcatttta ttgttcggca
    12541 aggtatcgac ggctttgatt aactgaccgg cgatgctctc tgcctctttt aacgtccggt
    12601 gcggtaacag cgcagcaaaa tcactgcggt ggtaacgcgc cagcagtgcg ccagggtagc
    12661 gcatcataaa tgtcgacagc agattcgtca gagtgaagaa ctgttcttca acctggctgt
    12721 gcccccaagt atcgctcaac atattgaaat ccggcagacg aatcatcatc acgatcccgt
    12781 gggtacctac tttctcctga tcttccagta acgttgctaa ctgattatcg aaaaagagtc
    12841 ggttattgag gccggttttc acgtcctggg cggcataaga gcggatcagc gtatcaagac
    12901 ggctgtgttg ttcgcgtgcg ttctgaattt cacgaagcag cgtatccagc gcactgctgg
    12961 ttctgggcgg ccattcatag atggttccca acacattaga gccacgctca ccgtttaaga
    13021 tacgagtagc ccgggtttcc agcaattctt gcccggcaag ttgccgttgt aaccagcgta
    13081 ccgccaagaa gagcataaca atgataaagc taatcgcccc cgtgagcggc gcggtggtca
    13141 tcaacgaatg gaaatagttg cccatcggat cctgataaac cagacgcagc gacatccccg
    13201 gatactttat caacggaacg ctcagttcac gaaacagatc gctggagcca accggacgat
    13261 aactaccatt tctggccagg gtataaacct gtttatcacc atggagcagg tctacacgaa
    13321 cgatatctgc cgacatcatt aattcggtaa tttgtggcct taatgtgctg aagtcattcg
    13381 acacaaggtg ggtatcaatc gccgtcgcca ccgcctgaac gcgatgacta aacttatact
    13441 gaattgcgtt gtagaaactt agcgaacagc ccagcaaagt cacaaaaatt gttaacccgg
    13501 tgagcagcgt aacaaaggcc gaaaatttcg tcgttaatct catccttgtg ttaactccga
    13561 tagtgaggaa gcgggcatac tagcaaatca gatttatctc gcaatttatt gcgcttcatc
    13621 ggctttgctt ttccattagc gagtataatc ttcagaaatt attttccaat ccatcatgca
    13681 catgaggacc acttatgcag gcgttacttt tagaacagca ggacggcaaa actctcgcat
    13741 cagtacagac tctggacgaa agtcgcctgc cggagggcga tgtcatggtc gatgttcact
    13801 ggtcgagcct gaactataaa gatgcgctgg cgattaccgg taagggaaaa atcatccgta
    13861 attttccgat gattcctggg atcgattttg ccggaactgt acgcaccagc gaagatccgc
    13921 gttttcatgc cggtcaggag gtgttactca ctggctgggg cgttggtgaa aaccactggg
    13981 gtgggctggc ggagcaggcg cgagtgaaag gtgactggct ggttgccatg ccgcaagggc
    14041 tggacgcgcg taaagcaata attatcggta ctgccggttt taccgccatg ctgtgtgtga
    14101 tggcgctgga agatgccggt gttcgcccgc aggacgggga gattgtcgtg acgggtgcca
    14161 gtggtggcgt cggcagtacc gccgtggcgc tgctgcataa gttgggttat caggtcgttg
    14221 ccgtttccgg tcgcgaaagt acccatgaat atctgaaaag tttaggtgcc agccgtattc
    14281 tccctcgtga tgagtttgcc gaatcccgtc ctctggaaaa acaagtctgg gctggggcaa
    14341 ttgacactgt tggcgacaaa gtgctggcaa aagtgctggc gcaaatgaat tacggcggct
    14401 gcgtggcggc ctgtggtctg gcgggtggtt ttactctgcc aaccacggtc atgccattta
    14461 ttctgcgtaa tgtccgtttg caaggggtgg attcagtaat gacgccacca gaacgccgcg
    14521 cacaagcctg gcagcgactg gtcgccgatt taccggaatc attctatacc caggcggcaa
    14581 aagagatatc tctgtcagag gcaccgaact ttgccgaggc catcattaat aaccagatcc
    14641 agggtcgcac gctggtgaag gttaactaac catttagcaa ggaataataa gagagggaac
    14701 tcattttgac atcattattc aatcggttaa cgggaaaagc ggttagccgg acagctttcg
    14761 tcgaacacct tggtcaggaa gttgtacaac atcatccaaa ctggaaagtc atgatttcga
    14821 ctgaccacaa attgatgcgc attgatactc ctctaaacag ccattattga tacgcctccg
    14881 tcgcctgtta ggtttatggt gctttgcctg ggcgacacta cgcttaacct gttactgatt
    14941 tctggtacca tggggtgaat aatctgattt tatttgactg caaattaacc actcgaacct
    15001 atttaatgct gagaattgtc agtcggttga ttttgcgtgc tttagcattc acatctatcc
    15061 agacgatgca gtgaaaattg ggtaatcccc agcaaccgct gcgtaatgtc gtctatcttg
    15121 tcgcgatcct ggcatcccta cattatttgt ggtctgtgaa gattatctca ttgcagcccc
    15181 tcatcttcgc agggctggct ttacagcttt tcaccttacg ttataagaag ttccgtcgat
    15241 gatggcgcta atttcgtgaa ttgtgcggct tgttgcaaat tacacggtgt tgaaggttat
    15301 ttacatgtta gctgttgatt atcttccctg ataagaccag tatttagctg ccaattgcta
    15361 cgaaatcgtt ataatgtgcg acctcgtcct ccctgacgca gtttttgcgc tgcggaaaag
    15421 gtgacattgg cgcaacgaag gtatattttg ttttttgccg gaggatagca gcagatcgct
    15481 gcacaatgtc cgtcaagtct aacattgata ctctggggca aaatagaccg gcgtcccggc
    15541 ctgctggaat ttatcgctat gcatacagct gtcggggcat acgctttaca gacggcggtg
    15601 aaacgcctgt cacaatcaca ctaaacaaag agtacggaac ccactcatgg atattcgtaa
    15661 gattaaaaaa ctgatcgagc tggttgaaga atcaggcatc tccgaactgg aaatttctga
    15721 aggcgaagag tcagtacgca ttagccgtgc agctcctgcc gcaagtttcc ctgtgatgca
    15781 acaagcttac gctgcaccaa tgatgcagca gccagctcaa tctaacgcag ccgctccggc
    15841 gaccgttcct tccatggaag cgccagcagc agcggaaatc agtggtcaca tcgtacgctc
    15901 cccgatggtt ggtactttct accgcacccc aagcccggac gcaaaagcgt tcatcgaagt
    15961 gggtcagaaa gtcaacgtgg gcgataccct gtgcatcgtt gaagccatga aaatgatgaa
    16021 ccagatcgaa gcggacaaat ccggtaccgt gaaagcaatt ctggtcgaaa gtggacaacc
    16081 ggtagaattt gacgagccgc tggtcgtcat cgagtaacga ggcgaacatg ctggataaaa
    16141 ttgttattgc caaccgcggc gagattgcat tgcgtattct tcgtgcctgt aaagaactgg
    16201 gcatcaagac tgtcgctgtg cactccagcg cggatcgcga tctaaaacac gtattactgg
    16261 cagatgaaac ggtctgtatt ggccctgctc cgtcagtaaa aagttatctg aacatcccgg
    16321 caatcatcag cgccgctgaa atcaccggcg cagtagcaat ccatccgggt tacggcttcc
    16381 tctccgagaa cgccaacttt gccgagcagg ttgaacgctc cggctttatc ttcattggcc
    16441 cgaaagcaga aaccattcgc ctgatgggcg acaaagtatc cgcaatcgcg gcgatgaaaa
    16501 aagcgggcgt cccttgcgta ccgggttctg acggcccgct gggcgacgat atggataaaa
    16561 accgtgccat tgctaaacgc attggttatc cggtgattat caaagcctcc ggcggcggtg
    16621 gtggtcgcgg tatgcgcgta gtgcgcggcg acgctgaact ggcacaatcc atctccatga
    16681 cccgtgcgga agcgaaagct gctttcagca acgatatggt ttacatggag aaatacctgg
    16741 aaaatcctcg ccacgtcgag attcaggtac tggctgacgg tcagggcaac gctatctatc
    16801 tggcggaacg tgactgctcc atgcagcgcc gccaccagaa agtggtcgaa gaagcaccag
    16861 caccgggcat taccccggaa ctacgtcgct acatcggcga acgttgcgct aaagcgtgtg
    16921 ttgatatcgg ctatcgcggt gcaggtactt tcgagttcct gttcgaaaac ggcgagttct
    16981 atttcatcga aatgaacacc cgtattcagg tagaacaccc ggttacagaa atgatcaccg
    17041 gcgttgacct gatcaaagaa cagctgcgta tcgctgccgg tcaaccgctg tcgatcaagc
    17101 aagaagaagt tcacgttcgc ggccatgcgg tggaatgtcg tatcaacgcc gaagatccga
    17161 acaccttcct gccaagccca ggcaaaatca cccgtttcca cgcacctggc ggttttggag
    17221 tgcgttggga gtctcatatc tacgcgggct acaccgtacc gccgtactat gactcaatga
    17281 tcggtaagct gatttgctac ggtgaaaacc gtgacgtggc gattgctcgc atgaagaatg
    17341 cgctgcagga gctgatcatc gacggtatca aaaccaacgt tgatctgcag atccgcatca
    17401 tgaatgacga gaacttccag catggtggca ctaacatcca ctatctggag aaaaaactcg
    17461 gtcttcagga aaaataattt gtgacgcgcg gtgttttcat cgcgcttagc aaagaacgta
    17521 atcaacgccg ggcctgttaa cgctgcccgg cgtttttatt gccgttagct aacggtagcc
    17581 tgcaatttcc gctttcaacg cgctcatttc agcatcgcca tcaacaattc ccagctcaaa
    17641 gcagtatttc tttgtttccc ctggctcaat aaagggcaga cgcccctgcg ctcgttcttt
    17701 atcacgaccg tcaggaaaac tattactgac ctccagtccc ataacatact cacccgcgcc
    17761 taaatttttc cactggataa agttgggtag ctgcatggtg tcgaatttct cgtaaattcc
    17821 ccaccccagt tcggcgttca ccagagcaac tttactcatc ccctgactgt cgctattcaa
    17881 cgtgagatag taaacctgct cgtcaaaacc gggctgtggg gtacaaatct ccagatgact
    17941 ggttaaccct tccgccgcat gcggagttcg tggcgttacg cttttagccg acagcaagat
    18001 ttctggcctg tggcgataat aaaggccagc cgtaattaaa gtgataaaga atttgcagcg
    18061 gctggcgggt gaaaccatga ttagtcacct tgtcttcaat gcgcagcaca ttttcgccat
    18121 aacggcagcg aatggtcctt tccagcgtca gattttcgcc gaacagtcgc gtttcccgta
    18181 ctttgccatt gatgacaaat tcaatgccgt cgtcggtccg ttctgtgcga taccccacct
    18241 cttctgctgg agtcgcagcc aaacgcccgt gtaaccccag cgtttctcct tcatcctcac
    18301 aaggcgtgcc aatgtagcta agtccgcagg tagtcagtaa ccccgcgtaa aagctacgta
    18361 aaaatccgtt gccctgagac tcaaaaaaag cgggggcgac aatgccgttg ggggtgataa
    18421 aagaaagcgc cgtgtccttg tagctggccc atgcaatatc cagcgcgcga tcttttacca
    18481 ccgtaaatgc cagtcccgca ccagtacgga catcaaacgc ttctgtaccc tttgctctgc
    18541 cactacgtaa ctcatagtgt ttaattccgg caacctgcga gagatcgcca atccacgacc
    18601 aaatttcgtt tttcattgtg ctgcttcccg aatggacatg tcatggcgct ggatgaacgc
    18661 ttcgatttcc gcaaggcgag acgttccctc cattgggcca cgtctggtta ccgccagcgc
    18721 accgcaggca ttggcatatt gcagcgctcg atggacgtca aaccctagct ggcggcaggc
    18781 aatccacgcg ccaccaaagc agtctcccgc accggtcggg tcaacttctt ccaccggata
    18841 gctctcaaca tgaaactgct cattcgcgga gtagtaactg gcaccttgat tgccgcgctt
    18901 cacaatcacc tctttaacac cttcttcaag aaaacctgcg attgcacgct ccggcgtcga
    18961 atgtggcgac aagagcaaca cttcgccctc gctgggcata tagatatccg ttaactcaag
    19021 aacaaagtgg agggcgtcac gcatttctgg aatatcgagc atctctttac gaatattagg
    19081 gtcgaaagaa ataacaccac catttgcttt aacgatagtt accgcttttt ttactgcgtc
    19141 taccatatga aacgaaaaca gggaagagcc cataatatga aaatgggtac agtccttcag
    19201 aatattttca tcaacatgct gcgccgataa tttaccgcaa gcggcattct taatattaaa
    19261 aataaaatcg cgatcgccag agttatgata agtaacaaag gcactcccgg tagcttccag
    19321 aggtaagact gaaatccccc tgatatccac accgtcagca gccagacgat gtatattaat
    19381 atcaccaaag ccatcattcc ccacacagct aatgatgccg cacggaacgc caagacgtgt
    19441 cacctggtcg ataaaaatag cgggcgcacc actgggataa gggccgttcc agatacctgg
    19501 ttgatcaaat ggctggccga ttttactcgc cataatttca accagaattt ctccaattgt
    19561 gaagacctta ctcatatgat taattccttg aaatgattag ctgcgttttc ttttatttct
    19621 tacgtcaaaa taaacggcta caagaatgac taatccttta accaccatct ggtaaaaata
    19681 gggaacattc attaagttca tgccattatt aataacgccg atagttaatg cgccgatcac
    19741 cgtccccacc acggttccat agccgccgtt caggctggtg cctcccagta cagccgcagc
    19801 aatagcatcc agttcgtagc tcaacgcgga attcggttac ccggaataga ggcgtgaggc
    19861 gaggatcatc ccgctgatac ctgccagcac ggctgtaatc ataaagactt taattttcaa
    19921 gcgggtgaca tttatccctg aataaattgc cgcttcttta ttaccaccag tgatataaat
    19981 ttttcgtcca aaggttgttt tagtaagaat gaagtggtta atcagcaata agatgattaa
    20041 tatccagatt ggaataggta tatagagaaa ttctccattc cccagtgcca gaaaggcatc
    20101 atctttaatc atgatgggca taccatcggt cactatataa gcgataccac ggaagatccc
    20161 catagtagca acggtgacaa taaacgacgg gatccccgct atagccgtca agccaccatt
    20221 tatcatcccc agagccagtg ctgcaataat ggataatggg accaataaaa taaatggaat
    20281 tccccatgaa gccccaagcg cagcaaatgt gccaatcaat gcaatggttg aaccgaccga
    20341 taaatcaata tcgccaagca aaagtacata agtcattcca taggcggtaa ttgcaattat
    20401 cgaaacctga agaataatat tagtaatgtt gttggcggta aaaaaactgt cattaataaa
    20461 actgaacaga atgattaaca ccaccattcc actgaggata ccgccatatt gcctgaagac
    20521 ttttttaaaa ttatttgtaa tgggaatttc cgaaggtttc ggactggcaa gagttttctc
    20581 tgtcatttaa cgtactcctg ttgcagcggt cattacctgt gtctgcgtta aattttcaca
    20641 tgaataaata tcggcaatcg ttttattacg cattaccacc agacgatcgc taagtgccat
    20701 gacttctggc agttcagagg acaccagaat aattgctgtc cctgccgcag ccagttgacg
    20761 tatcacgtta tagatttcaa attttgcgcc gacatccact ccgcgcgttg gctcatcgag
    20821 caataaaata cgcggagttt tctccagcca cttcgataac acgattttct gttgattacc
    20881 gccgctcagc gctcccactg cctgatgtac agaactcact ttagtgttca ttctggcaat
    20941 tgattgcagc gtcttcgcct cttcttgtcg gtgattgatg agtcccatcc gcgaagcgtt
    21001 atattgaaac gccacatggt gcgtgttcat attgacgtcg tgcatcagca ctaacccttc
    21061 ttccttgcgg ttttcggtca caaaagcaat gccatgatca atagcctgtt taggattagt
    21121 gatcctgact tccttaccat caatcaacac agtcccctga tacgaaacga ggccaaagag
    21181 cgccttcatc acatcggaac gcccggcccc caccaggcca aaaaatccga ggacctcccc
    21241 cggacgcaca gcgaaactga tctctttaaa aagcgaagga tgggaaagat tttttacttc
    21301 cagttttgca ggaacatttt catccggtat ttccccaaga cgtgctggcc aaatattttt
    21361 catttcccga ccaaccattt gggcaatcaa atgctggata tcactgtttt tcttttcatg
    21421 cgtggcgata tactcaccgt cgcggatcac cgaaatatca tctgaaatcc gcataatttc
    21481 atccatgcga tgagagatat aaataaccgc acagcctcgc tgctttaatt tttcaataat
    21541 gttaaacaga atctccgctt cattatcact tagcgaagat gtcggttcat ccataatcac
    21601 gacatccgct gcatagctta aggctttagc aatttctacc agttgcctat gtgccatcga
    21661 catattgccg accatttcta gtgggtctag tggtatgccc cgttccgcga ggagattctg
    21721 gcaatctgcg agcatttttt tatcgttaat aatgccaaaa cgtcgcggtt cattattggc
    21781 ataaatattt tccgccacgg tcatattatt actcaggctc aattcctgaa aaataatcgc
    21841 aattcctaac tggcgtgaat gctgcggatt attaatggtg atagtttcac cacgtagtgt
    21901 tatcgttccc tgggttggcg gataaacacc ggccagtatt ttcattaatg tcgatttacc
    21961 ggcaccattc tcgcctaata aagtatgcac ccgcccttta cgtaaagaga gggtaatatc
    22021 ttttaatgct tgaacatgac caaatgtctt cgcaatatgc gaaacatcaa gaataacatc
    22081 cgtcataatg acctcggtgt cacaacaaaa cgccaataac gttttgttgt gaggtgaaga
    22141 tgtttactat ttacctgtat aaacgccggg ttgtaccggg atcatttttt ctaccggttt
    22201 accattgagg tgatcaacag cggcatcaat ggctttagca cccatttgat cgggatattg
    22261 ccgaatcacg gccgcaaacg ttttttcact gtcaacggca tcacgcgctt ctttcatgcc
    22321 atcaaaacca atgattttga tgttgtcgtt atgcgcagat tttgctgcga taaccgctgc
    22381 cagcgccgca tcatcaccaa agccaaacag accattcagg ttggcatttg cctgcataat
    22441 gttttgcgca gtcgttaacg cttcagcccg cgtaatacta ggctgttcag caacaatttt
    22501 aatatccggt gtatcgctta accctttctt aaaaccatca acgcgcgcaa ccaccgattg
    22561 tagtgccgga taagaaataa tggctacagt acctttacca ttcaaaagtt tgcccattaa
    22621 cttgcctgct tcaacgcctc ccgcatagtt atcggtcgca acatgcgata cgaccggaac
    22681 gccttccgca gcgacgtcga cggtgataac gggaataccg gccttatcgg ctttaattat
    22741 cgccgcctgg acgcctttcg aatcgaccgg agagataata atggcatcca cttttttagt
    22801 aataaaatcc tcaacatcag aaagttgttt gtttaaatcc tgat
//
LOCUS       DAJTNN010000055        22585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTNN010000055 DAJTNN010000000
VERSION     DAJTNN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.055"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..257)
                     /locus_tag="OO150_001851"
     CDS             complement(<1..257)
                     /locus_tag="OO150_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5516019.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDC"
     gene            complement(248..718)
                     /locus_tag="OO150_001852"
     CDS             complement(248..718)
                     /locus_tag="OO150_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5516020.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(903..1697)
                     /gene="thyA"
                     /locus_tag="OO150_001853"
     CDS             complement(903..1697)
                     /gene="thyA"
                     /locus_tag="OO150_001853"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5516021.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYAPHPGIKAPVAI"
     gene            complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="OO150_001854"
     CDS             complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="OO150_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5516022.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="OO150_001855"
     CDS             complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="OO150_001855"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5516023.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGLPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPILLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVVAFMERRGM
                     GGLIRGGL"
     gene            complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="OO150_001856"
     CDS             complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="OO150_001856"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5516024.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            6204..6893
                     /gene="mutH"
                     /locus_tag="OO150_001857"
     CDS             6204..6893
                     /gene="mutH"
                     /locus_tag="OO150_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5516025.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            6962..7675
                     /locus_tag="OO150_001858"
     CDS             6962..7675
                     /locus_tag="OO150_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5516026.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            7813..8031
                     /gene="ygdR"
                     /locus_tag="OO150_001859"
     CDS             7813..8031
                     /gene="ygdR"
                     /locus_tag="OO150_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5516027.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            8139..9179
                     /locus_tag="OO150_001860"
     CDS             8139..9179
                     /locus_tag="OO150_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5516028.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLNLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGGMRYLHLADKHDLPSIVTIQTPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKTAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="OO150_001861"
     CDS             complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="OO150_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5516029.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(10397..12556)
                     /gene="aas"
                     /locus_tag="OO150_001862"
     CDS             complement(10397..12556)
                     /gene="aas"
                     /locus_tag="OO150_001862"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5516030.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTLDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            12945..13976
                     /gene="galR"
                     /locus_tag="OO150_001863"
     CDS             12945..13976
                     /gene="galR"
                     /locus_tag="OO150_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5516031.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(13983..15245)
                     /gene="lysA"
                     /locus_tag="OO150_001864"
     CDS             complement(13983..15245)
                     /gene="lysA"
                     /locus_tag="OO150_001864"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5516032.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            15367..16302
                     /gene="lysR"
                     /locus_tag="OO150_001865"
     CDS             15367..16302
                     /gene="lysR"
                     /locus_tag="OO150_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5516033.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(16289..16981)
                     /gene="ygeA"
                     /locus_tag="OO150_001866"
     CDS             complement(16289..16981)
                     /gene="ygeA"
                     /locus_tag="OO150_001866"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5516034.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(17110..18525)
                     /gene="araE"
                     /locus_tag="OO150_001867"
                     /pseudo
     CDS             complement(17110..18525)
                     /gene="araE"
                     /locus_tag="OO150_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(18840..19601)
                     /gene="kduD"
                     /locus_tag="OO150_001868"
     CDS             complement(18840..19601)
                     /gene="kduD"
                     /locus_tag="OO150_001868"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5516035.1"
                     /translation="MILSAFSLEGKVAVVTGCDTWLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(19631..20467)
                     /gene="kduI"
                     /locus_tag="OO150_001869"
     CDS             complement(19631..20467)
                     /gene="kduI"
                     /locus_tag="OO150_001869"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5516036.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(20754..21935)
                     /locus_tag="OO150_001870"
     CDS             complement(20754..21935)
                     /locus_tag="OO150_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5516037.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            22190..>22585
                     /locus_tag="OO150_001871"
     CDS             22190..>22585
                     /locus_tag="OO150_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5516038.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSL"
BASE COUNT         5792 a         6049 c         5440 g         5304 t
ORIGIN      
        1 atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagatgcc atgacaataa
       61 cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagc
      121 gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc
      181 cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct
      241 tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt cttccccacg
      301 gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg
      361 cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg
      421 gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg
      481 aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat
      541 gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg gcggtttgcc
      601 atagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga
      661 ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc
      721 cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc
      781 ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggtg tctcgaagaa
      841 tttaatggag ggtaaaaaaa accgacgcac actagcgtcg gctctggcag gatgtttcgt
      901 aattagatag ccaccggcgc tttaatgccc ggatgcggag cgtagccttc aatctcaaaa
      961 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
     1021 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct gtacaggtgc
     1081 gtgtcgcccc ccgtccagac aaaatcgccc acttccagat cacactgctg cgccatcata
     1141 tgcaccagta acgcgtagct ggcaatgtta aacggcaggc cgaggaaaac gtcacaggag
     1201 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaacgcatgg
     1261 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
     1321 cgcgaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
     1381 ccatccggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccgttt
     1441 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
     1501 tcgccctgca gaaaccacag cagttcatgg atgatggaac gcaggtggca acgtttagtt
     1561 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
     1621 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
     1681 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
     1741 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg tcccatgctg
     1801 atgtactgca cccaggcacc agtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
     1861 atgcgaaacg cgccgtaacc aatcaggaac aaacctgaga cagctcccat tgggcgcggt
     1921 ttacgaatat acaggttgag gataataaac agcactacac cttccagcaa cagctcgtaa
     1981 agctgtgatg ggtggcgcgg cagcacaccg taagtatcga aaatggattg ccactgcggg
     2041 ttggtttgca gcagcaaaat atcttctgta cgggagccag ggaacagcat ggcaaacggg
     2101 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
     2161 ccaagaccaa acggaatgag tggtgcgata aaatcagaga cctggaagaa ggaacgttta
     2221 gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaggac
     2281 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaagttg
     2341 tagaacagaa cataaccaat acgtccaccg aggaagacgc cgaggaagcc cgcatagagt
     2401 aagttttcaa cttcattttt ggtccagcca ctgcccggac gattcgcccg acgtgttgcc
     2461 agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg aagcgccacg
     2521 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
     2581 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
     2641 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
     2701 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
     2761 ccgcgacgct ccataaaggc tacaacctga tgacgaactt cggtcgccag ttgcgcttcc
     2821 agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt
     2881 acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
     2941 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat accatgtatt
     3001 tccgcttccc gggcgatcat tgccagagct cgtaacattg caggatgaag gctgtcataa
     3061 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
     3121 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa taccattgac
     3181 ggtacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
     3241 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc gaggctggtg
     3301 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
     3361 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
     3421 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
     3481 gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc
     3541 tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga
     3601 ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accagcattg
     3661 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
     3721 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtatc
     3781 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
     3841 agcaccgaag gctgaatatc cgcgcccatc acggtaggga gccccagcgc acgtaccatg
     3901 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
     3961 ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
     4021 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
     4081 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
     4141 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
     4201 aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc
     4261 gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
     4321 gcttcttcca gcgccccggt caatcgttcg cgttccagag ccggatccag cgttgatgcc
     4381 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
     4441 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa caaggcagtc
     4501 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
     4561 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
     4621 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
     4681 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
     4741 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
     4801 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
     4861 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
     4921 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
     4981 ggcgtgactt aacctctttt acgtcgataa gcagatgtgt tttgtggttt tggcgtattt
     5041 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
     5101 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
     5161 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag ctgcaagaga
     5221 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
     5281 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
     5341 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
     5401 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
     5461 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
     5521 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt
     5581 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
     5641 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
     5701 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
     5761 taggcatttt aaaatattca ttataagtca atgcattaca tgtaatttga gtaatgaaaa
     5821 agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat
     5881 gccgtattta tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt
     5941 ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac agttatccca
     6001 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
     6061 cctattgcag tgctgcgcaa ctcgattatc ggcagcctaa aaagcaatat aaatcagaga
     6121 attgaacaac acatgtggaa aaagttacac tgcgaatatt cggtacataa ttgctgtttg
     6181 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actgctctct cctcccgaaa
     6241 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
     6301 cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg
     6361 tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg
     6421 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
     6481 cattcgtttg tgttgccccg ttaacgggta atagcggggt gacctgggaa accagccacg
     6541 tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggcgagcgc agcatcccgc
     6601 tggcgcagcg tcgcgtagga tccccgttgc tgtggacccc gaatgaagag gaagaccgac
     6661 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
     6721 tcaccgctcg tcacggggaa tatttacaga taagaccgaa agcagcgaat gcgaaagcgc
     6781 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
     6841 tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc
     6901 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
     6961 gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta cgctgacgct
     7021 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
     7081 tcccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
     7141 tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc tttttacaat
     7201 attcagtcag gaaatttctg cccgtgattt gattctgctt ctgggtggct tgttccttat
     7261 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
     7321 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatctttag
     7381 cctcgactcg gtgattacgg ctgtgggtct gtcagatcac ctgtttatta tgatggcagc
     7441 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
     7501 acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtgg gctttaccct
     7561 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
     7621 ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa aagaatccgc tctgatactc
     7681 cgtacgctct cctgcgggag ggcgtactca ctccttttac cctcacagtt taagattttc
     7741 ctgctttcaa gatatataac gtcgttttat aaacagacta ttatcatagg taggccaaat
     7801 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
     7861 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
     7921 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
     7981 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
     8041 tcagcatccg gctggcctta agatttttct ctttcccttt tcccttccct ctgccatttt
     8101 tatattcctt atgtcgtgat tataaaaagg aaacggctat gcaatatcac cgtatacccc
     8161 acagttcgct ggaagtcagc acgctggggc ttggcacgat gacgtttggt gaacagaaca
     8221 gcgaagccga cgcccacgca caactcgact atgccgtcgc tcagggcatt aaccttatcg
     8281 acgttgccga aatgtaccca gtacctccgc gccccgaaac gcaagggtta accgaaacct
     8341 acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa gttaattatc gcctccaaag
     8401 tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg ctggatcgga
     8461 agaatatccg cgaagcgctg catgacagcc tcaagcgcct acagactgat tacctcaatc
     8521 tttatcaggt gcactggccg cagcgcccga ccaactgctt cggcaaactc ggttatagct
     8581 ggacggattc tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg gcagagtacc
     8641 aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga aactgcattt ggcggaatgc
     8701 gctacctgca tctggcggac aaacacgatc tgccgagtat tgtcaccatt cagacccctt
     8761 acagtctgtt aaaccgcagt tttgaagtag gtctggcaga agtcagccag tatgaagggg
     8821 tcgaactgct ggcctattca tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg
     8881 gtgcaaaaac cgctggcgca cgtaatacgc tctttagtcg gttcacccgc tatagcggtg
     8941 agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat ggcctggacc
     9001 ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg
     9061 gcgcaaccac gatggatcag ctgaaaacta acatcgaaag tttgcatctg gagttaagcg
     9121 aagacgtatt agctgaaatt gaagcggtgc atcaggttta tacttatccg gcaccataag
     9181 attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc
     9241 cgttattgcc agcgcaaaca gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc
     9301 taccattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag
     9361 gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa
     9421 cgcattgagc ggaacgacaa aaaagccccc catcacgcca atcagcatca acaaggcata
     9481 ggctggcagc agctcgtgtt gcagggaaaa aatcagtacc accacgccaa tcaaaatccc
     9541 ggctggcata cagcgtgaca cggtttccag cgtaactaac ttcgctgccg cacctgcgcc
     9601 aaccacgata ccaatcgcta ccatcgcgtt gagataggtg ggcgtagcgt tatcggtaat
     9661 gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgtgacac ccgctcccca
     9721 gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcgccata gcgaggtgca
     9781 ggcattcagg aaactgcggg tcatgttgat gagattccaa gactgccccg gacgcgccgc
     9841 cgccagtttg ggaatgtaga tattggcaac gaccgcacca ccgtaggcca gtgcgcatgc
     9901 ggccagggcg acgaggacat gccagtcagc cagcacacca ccggctacgg aaccgagcaa
     9961 aatcgccgct atggtagaag cttccattaa accgttagct ttcactaact tactacccgt
    10021 ggttaattcg ccgagaatac cgtatttcgc cggtgaatag gctgcagcac caacccccac
    10081 cagcgtatag ccgagaaacg gattgatacc aaagcagata ctggctgcgc ccagcagctt
    10141 caggccgttg gcaaacatca tcacccggcc tttggcgaag ctatccgcca cctgcccgac
    10201 aaacggcgca aaaagaatgt aagcacctac aaacaccatt tgcaggatgg gctggctcca
    10261 ctccggatag aactgcgctt tcagtaacgc cagagtggca aacagtaggg cattatcgcc
    10321 aaacgcagag agaaactgcg ccacgataac cgctttcatc cccttcgacc acaacgaagt
    10381 gttagtgtgc actgactcac tcatcgtgtt gttccgcttc gtctacccag cttttcaacg
    10441 tgacaaagtc aggtttgccg ctgccaagta atggcatctg tttcagatag cgaatatcgc
    10501 gcggtacagc aagctccggc acgccgtgct cgcgggcata ctgttgcaac ttatcgagcg
    10561 tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa
    10621 tcgcagtggc atggacttta tctggcgaaa caccaagtgc cagttgttcc accatttcca
    10681 gcgacaccat ttcgcctgca attttggcaa agcgttttgc gcggccctga atctgcacaa
    10741 agccctgctc gtcaaaacgc acaatatcgc cagtgtcata ccagccgcgc tccatttcgc
    10801 cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg cttctccacc cgcagatagc
    10861 cgttcattat gttcggccct ttcagttgca ggcgtccgcc ctcttcgata ccaggaaccg
    10921 acaacaggcg cgcatccatt cctggtagaa tacgccctac cgtaccgggt ttcgccgcca
    10981 tcggtacgtt gatagaaacg acaggcgcgc actcggttac accatagcct tcaaggatgc
    11041 gcaggccaaa tttatcctgc caaagctgtt tggtactttc ttgtagtttt tctgcgcctg
    11101 ccaccacata gcgtagacga tagaagtcat acgggttggc gaaacgcgcg tagtgaccga
    11161 ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc atacaccaac tccggcacaa
    11221 tgcggtaatg cagcgggctt ggataaagga acacttctgc acctgtaagc agtggcgtaa
    11281 acaggcctac cgtcagccca aaggagtgaa acagcggtaa tgccgacata aagcgatcgt
    11341 tggtggtgaa gtcggcaatc gttttaatct gctcgacgtt cgccagaatg cttttatggc
    11401 tatggacgac gccttttgga tggccttcag aaccggaggt aaagaggatc agcgcctctt
    11461 cttccggctg ctgttttacc tgcgccaggc gcggcatcag caaatgagcg aagatccata
    11521 ctttgtcggc agtggtgaca tctgctttta aatcttccag atagacccag cgcacctgag
    11581 taagttgctc cggcagatgc cagagtttgc ctttatcgag aaactggcgg gaagtgaaga
    11641 tggttttgat ttcagccgcc gtaatagcac tggtcagccc ttttacccca gcagtgtagt
    11701 tcatcattgc gggaatgcgg cgacgcgcga tggccccaaa aatcactgcc gcactgatgc
    11761 ctgcattggg cagcattaag ccgatgcgtt cgccttcaac actgtatttt tcaaggatgc
    11821 gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga gtctggggta aagttgacgt
    11881 cttcgacaca tttcttcccg gctccgaagc ggtacattgc actcagtaaa gattcgtaca
    11941 gagtttcacg cgggcgcacc gccatacgcg cttccatcat tatttgatgc agcatttcgc
    12001 cagcgatttt gcgacggtca cgggcacgcg acgcatccgg catcgccacc tgcgttggag
    12061 gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt aaccagacct ttcaggcggc
    12121 tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat aaccgttgca ccagacttcg
    12181 ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga gcctgtagtg gtgatgcgtc
    12241 cttcagggaa aatcaccact ggtcggccct gttccaccag acgtaccaga tgtttaatag
    12301 ccataggttg cgtcgggtcg agaggaacaa agtcgataaa tgatttcagc caacgcatat
    12361 accactgttg gcttattgag gtgtaaacgg caaacactgg acgcacaggt aaaaacagtc
    12421 caagcaaaat gccatcaata aaagagacgt gattaggcgt aattagaacg cgctcgccct
    12481 tcagtgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa aacacggcac aaatttcgaa
    12541 aaaagctaaa aagcattcca actccctttg ctctgattca gtaaaagcga atggagggag
    12601 attacacgag ataaagaacg cgagcgacag taaattaggt gcgaaaaaaa acctgcgcat
    12661 ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta ccgaataatc tcaccaatca
    12721 atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca
    12781 acagcgtttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg
    12841 cgtttggctc acattcccac gatgaaaaca cgccactcct tgaaccaacg ggcgttttcc
    12901 gtaacactga aagaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg
    12961 atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac
    13021 ccaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc
    13081 acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg
    13141 ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt
    13201 atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa aaagagcgtc
    13261 aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga
    13321 tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc
    13381 gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc
    13441 tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc
    13501 actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg
    13561 gtattgcggc caatgaccgg ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac
    13621 aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg
    13681 attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg
    13741 agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga
    13801 ccaccgtgcg ttacccaatc gtgacgatgg cgacccaggc tgccgaactg gctttggcgc
    13861 tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg ctggtacgtc
    13921 gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc
    13981 agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc
    14041 gcctgaccat tatcaaacag aacttctggt aacagcgaac ggctattgta gttggatgac
    14101 attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc cttcacttcc
    14161 ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca
    14221 cataacggtc cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca
    14281 gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg
    14341 aacccggcat caaccagcac aaagtggcgg ctccccattt gtttgacgct ccgcacctga
    14401 gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc cagtttcaca
    14461 gggtggccca aatggcgggc gatttgctca cgcgcggcat tccacagacc ataataatgt
    14521 tcggtatcaa ccgcctcttc accctgttga taaggaacag aaagcccacc gcccgcagaa
    14581 atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc acacacctgt
    14641 tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac cagctgcaga
    14701 tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg
    14761 ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cggattaacg
    14821 cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa
    14881 cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca
    14941 tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca
    15001 cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc
    15061 aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc
    15121 gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca
    15181 gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt
    15241 ggcataacaa actccagata agtgcttttt tatgattacg ccacatcata aaaagaataa
    15301 aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga ttgcgagaga
    15361 gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc
    15421 ggaagcctga ctgaggcggc acacctgcta cacacctcac agccaaccgt cagccgcgaa
    15481 cttgcgcgct ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg tgggcgatta
    15541 catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg
    15601 gatcgaattg tcagcgctgc agaaagtctg cgcgagtttc gccagggaga actgtctatt
    15661 gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca
    15721 cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg
    15781 ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc tgcgggaaca
    15841 gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg
    15901 gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat cagcctttcc
    15961 cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca ggttaaacga
    16021 cgcatgatcg tagaaaccca cagcgccgcg tcaatctgcg caatggtacg ggcgggggta
    16081 ggtgtttcgg tggttaaccc gctcaccgca ctggattatg cggcaagcgg tttagtggtg
    16141 cggcggttca gtattgcggt tccgttcaca gtcagcctga tccgccccct gcaccgcccg
    16201 tcatcagcgc tggtgcaggc gtttagtgag catttacaag cggggttacc gaaactggtc
    16261 acttctcttg acgctatttt gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg
    16321 catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctggc accagtaaac
    16381 caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg cgagcaatca
    16441 cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt tcttcaaaaa
    16501 taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg atggaaaatt
    16561 gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta cccagcagcg
    16621 ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca atgtgtaaga
    16681 aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta ttggtacata
    16741 acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatgtccc
    16801 cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat
    16861 gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca
    16921 aacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca attgttttca
    16981 ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct
    17041 caacggccaa gtgcccaatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg
    17101 cccgtgaaat cagatgccga tatttctcaa cttctcgcct gccatcagtt tgcgttcgat
    17161 atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtgatgc ccacaaacgc
    17221 aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctgt cgagcagcgt
    17281 caggaaggtc gccgataatc atattcgaca cccagttcgt ggtggtcgaa caggtaatac
    17341 cgaaatcgcg gcatttcagc ggctgaattt cagagcacag gatccacacc actggcgcgg
    17401 cgctcatcgc ataaccggca atacacatca tcgtcatgcc aacagagagc caggacaatc
    17461 cactggaagc cgtaccgtta tcaaactgca tcaggcaata gcccagcacc agagtgccta
    17521 acgccatcac gctgaaacca attttcagag ccggtttacc cctgctttat ctaccgtaaa
    17581 caccgcaata aaggtggcga acataaaggt cagccctacg accagagtcg caatcatctg
    17641 ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc ggcgcgtagt acatgatgat
    17701 gttcataccg gtaaactgct gcatcgcctg caacaacata ccgagaaaca cagcacgacg
    17761 gacgttacgg ttgatcttaa acagtgccca accgccctgt tttaacttca ggctttcacg
    17821 aatttcgttg agttcttctc gcgctttttc cgacgtatcg cgcagcatac gcaatacttc
    17881 ttccgcctca atatgacgcc ccttttccgc caaccagcgc gggctatttg gcaggaagac
    17941 caccagaata atcagcagaa ctgctggtaa agcaagaacc cccaacattg cgcgccagtt
    18001 accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat
    18061 caactggtac atactgatca tcttaccgcg aacgttttca cttgccattt cagaaagata
    18121 cagaggagcg gtgtaagacg cgatcccgac agcaatgccc agccccacac gagcggcgat
    18181 taacatctct acgctggtcg caaaagcgga ccctatagaa ccgagtacaa acaggatggc
    18241 ccccgccatc aggctgtatt tacgccccag gcggaacgac agccaaccat taaacagcgc
    18301 accaattgct gcgccgagca tcatgctact aaccacccat tcctgcaaat gactggtcag
    18361 cacaaagtga tcggtaatga acggcaacgc tccggcgatt acgccgatat caagaccaaa
    18421 taacaatcct gcgaccgcag cagctaccga aacaaacata ttcatacgcc gcgtatcccg
    18481 caaagaacgt ggcgttaaag cagattccgt attgatagta accatttttt cctgccagca
    18541 gagagtaaga catagtgaaa aaatacgtga acaactcacg caggtgtcag gtcggaaaca
    18601 gcataaatat ggattaaatt gctgcgacat gtcgttatgt gatggatatt ccaattttca
    18661 aattaagttg aattattgag attattatta accacctaat tttacagcag ataaaattca
    18721 taaagttcat taattgataa ttaatatgga ttatttcata accatgatat ggattatgat
    18781 gatctacagg tataaaaaac cctgccatgc ggcagggtca taaaagtaag aagaatgaat
    18841 taacgcgcca gccaaccgcc atccacggca atggtataac cattcacata atctgaagcg
    18901 ctggaggcaa ggaacactat cggccccatc aggtcactcg gcagtcccca acgaccagct
    18961 ggaatgcggt cgagaatttc cgcgctacgt tgttcatctg cccgtagttg ttgagtattg
    19021 ttggtcgcca tgtaacccgg agctatcgca ttaacattaa tgttgtgttt agcccattcg
    19081 ttcgccatca atcgcgtcac acccatcacg ccgctttttg atgcggtata agaaggcaca
    19141 cggatcccgc cctggaagga gagcattgac gcgatattga taatcttgcc gccattgcct
    19201 tgcgcgataa agtgtttcgc cgctgcctga gacatgaaga atacgctctt gatattcagg
    19261 ttcatgacat cgtcccagtc tttttcgctg aactcgagag cgtcttcgcg gcgaatcaat
    19321 ccggcgttat tcaccaggat atcaatatga ccaaattcag ctaccgcgcg atccagcagt
    19381 ccaggaatgc catcaatctt tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc
    19441 gccgtgacct gctcgatggt ttcagtcggt tcaacgatgt taatgccaac aatgtcacag
    19501 cccgcttgcg ccagccccaa cgccatcccc tggcccagcc aagtatcaca accagtgacg
    19561 accgcaactt taccttcgag agaaaatgca cttaaaatca taacaatacc ttattcgttt
    19621 atgcccacaa ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt
    19681 tttcaccaac catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg
    19741 accagctcgg ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg
    19801 gttgccccat catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt
    19861 ccatccggcg ctcgtgggtg tgacacggca tggtgttcca caagttacct ggagccagct
    19921 ccgtcagccc catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa
    19981 tagtgcgacg gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccgggg
    20041 tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg
    20101 gagtggcggt atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg
    20161 cgtcgcggtg accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa
    20221 tattgataac acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa
    20281 cttccccgcc aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt
    20341 caatgtggct gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt
    20401 cattgtgcag cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ctctgtctta
    20461 cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac
    20521 tgtttcgttt tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat
    20581 aaaacaatgt tttattttta agttaaggat taaaaaaagt gcagtgtcga tcacgaatat
    20641 gtcatgagaa tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat
    20701 tattcaacag attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg
    20761 tcacgttcaa tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct
    20821 ttgcgggcat tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa
    20881 gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc
    20941 cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata
    21001 agatcgacat cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc
    21061 ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc
    21121 agcacgggta aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg
    21181 ccatcgttta tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga
    21241 tttaaacggg ctaaaccttc tgcactggcg tcagtgcatg gctgttcatc ggtatcaaca
    21301 accaacgtct gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg
    21361 gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga
    21421 cggctgatgc catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg
    21481 aaggcatccc acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt
    21541 gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg
    21601 gcgatgacaa tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta
    21661 agcccagaac cgcaaacgtc attaatagtg attgcagaaa cgctattagg aagaccacct
    21721 ttaatagccg attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga
    21781 attacttcat ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc
    21841 aaactaccaa gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt
    21901 gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg
    21961 caatagtaaa tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc
    22021 acaaaagaaa tatgcctgag cagcagtcag agacataact ggcacgtaag gtttgcaacc
    22081 actaacccac caatagaggg gtagataggg cgttaatctc ccatacttaa cctggtttat
    22141 ggtaaattgc cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat
    22201 ttggtctaaa gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg
    22261 cacgctcttc cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc
    22321 tctggtcgcc tggcctttaa catattggcc acataaagct ttatgccagt tcatcctctc
    22381 atcgaaaaca tcagcaggtg aagggataac gggcgcggta acacactact atggcaagaa
    22441 gattggtaat ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata
    22501 tgcagtggca attaccaact cacttacaga acagctggca aagcatatgg ttattgatct
    22561 tcgcatccgt atgttggtga gtctg
//
LOCUS       DAJTNN010000056        22444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTNN010000056 DAJTNN010000000
VERSION     DAJTNN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.056"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            41..232
                     /locus_tag="OO150_001872"
     CDS             41..232
                     /locus_tag="OO150_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5516039.1"
                     /translation="MKYGKLGYIVPRPQKIRGRWLIDRRAVFVGPGETGIAPEIHTGD
                     DDALKEILTHVTEATKKQH"
     gene            201..1307
                     /locus_tag="OO150_001873"
     CDS             201..1307
                     /locus_tag="OO150_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5516040.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWV"
     gene            1487..2281
                     /gene="potC"
                     /locus_tag="OO150_001874"
     CDS             1487..2281
                     /gene="potC"
                     /locus_tag="OO150_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5516041.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMISPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2278..3324
                     /gene="potD"
                     /locus_tag="OO150_001875"
     CDS             2278..3324
                     /gene="potD"
                     /locus_tag="OO150_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5516042.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETCIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3480..4301)
                     /gene="cobB"
                     /locus_tag="OO150_001876"
     CDS             complement(3480..4301)
                     /gene="cobB"
                     /locus_tag="OO150_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5516043.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     KIQPNAAHLALAKLQEALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4317..5228)
                     /gene="nagK"
                     /locus_tag="OO150_001877"
     CDS             complement(4317..5228)
                     /gene="nagK"
                     /locus_tag="OO150_001877"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5516044.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5257..6501)
                     /gene="lolE"
                     /locus_tag="OO150_001878"
     CDS             complement(5257..6501)
                     /gene="lolE"
                     /locus_tag="OO150_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5516045.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6501..7202)
                     /gene="lolD"
                     /locus_tag="OO150_001879"
     CDS             complement(6501..7202)
                     /gene="lolD"
                     /locus_tag="OO150_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5516046.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7195..8394)
                     /gene="lolC"
                     /locus_tag="OO150_001880"
     CDS             complement(7195..8394)
                     /gene="lolC"
                     /locus_tag="OO150_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5516047.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8656..9729
                     /locus_tag="OO150_001881"
     CDS             8656..9729
                     /locus_tag="OO150_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5516048.1"
                     /translation="MKQKELWINQIKGLCICPVVIYHSVITFYPHMTTFPHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9857..13303
                     /gene="mfd"
                     /locus_tag="OO150_001882"
     CDS             9857..13303
                     /gene="mfd"
                     /locus_tag="OO150_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5516049.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13447..14409
                     /gene="ldtC"
                     /locus_tag="OO150_001883"
     CDS             13447..14409
                     /gene="ldtC"
                     /locus_tag="OO150_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5516050.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     TRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14492..14749)
                     /gene="bhsA"
                     /locus_tag="OO150_001884"
     CDS             complement(14492..14749)
                     /gene="bhsA"
                     /locus_tag="OO150_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR5516051.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            14990..15622
                     /gene="comR"
                     /locus_tag="OO150_001885"
     CDS             14990..15622
                     /gene="comR"
                     /locus_tag="OO150_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5516052.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15684..16223)
                     /locus_tag="OO150_001886"
     CDS             complement(15684..16223)
                     /locus_tag="OO150_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5516053.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16450..17754)
                     /gene="ndh"
                     /locus_tag="OO150_001887"
     CDS             complement(16450..17754)
                     /gene="ndh"
                     /locus_tag="OO150_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5516054.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTHDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(18154..18696)
                     /locus_tag="OO150_001888"
     CDS             complement(18154..18696)
                     /locus_tag="OO150_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5516055.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18719..19744)
                     /gene="nagZ"
                     /locus_tag="OO150_001889"
     CDS             complement(18719..19744)
                     /gene="nagZ"
                     /locus_tag="OO150_001889"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5516056.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19755..20579)
                     /gene="thiK"
                     /locus_tag="OO150_001890"
     CDS             complement(19755..20579)
                     /gene="thiK"
                     /locus_tag="OO150_001890"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5516057.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20563..21201)
                     /gene="lpoB"
                     /locus_tag="OO150_001891"
     CDS             complement(20563..21201)
                     /gene="lpoB"
                     /locus_tag="OO150_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5516058.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPIVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(21215..21592)
                     /locus_tag="OO150_001892"
     CDS             complement(21215..21592)
                     /locus_tag="OO150_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5516059.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21595..21954)
                     /gene="hinT"
                     /locus_tag="OO150_001893"
     CDS             complement(21595..21954)
                     /gene="hinT"
                     /locus_tag="OO150_001893"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5516060.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVVAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
BASE COUNT         5589 a         5893 c         5572 g         5390 t
ORIGIN      
        1 cgacgagtgg aaaccaaaac ggttcagtct cactaccctg gtgaaatacg gaaaactagg
       61 atacatcgtt ccaagaccac aaaaaattcg tgggcgttgg ctgatagatc gccgagcagt
      121 atttgttggg ccaggtgaaa cgggaattgc gccggaaatt catactggcg atgatgatgc
      181 actgaaggag attttaactc atgtcaccga ggccacgaaa aaacagcact gacgtagccg
      241 gtctttacga aaagtttgat cgcagaactg gcagagttta ctaccagtat aaaaatcctg
      301 tgactggaaa atttcacgga ctcggaacag acaaaggtaa ggcagaaaaa atcgcttcca
      361 cagccaatca gcgaatagct gcagcagaag ctgaatattt catgcgcaaa attgatgaaa
      421 gtccgtcagc aacaaaacgt cggggtatca gattaaaggc atgggttgat cgatatctga
      481 aaatacagga cacgcgactg aaaaatggag atattgcagc tacaactcac aaagaaaaaa
      541 ctcgaatggc tgcatacctg gtttcccgtc tgggaaacca cccattgaaa gaactggaag
      601 taagagactt tgcattaata ctggatgagt ggctggataa agacatggtc agcacagcga
      661 gagtaaatcg tggattatgg gttgatattt ataaagaagc acagcatgca ggggaagttc
      721 ctcctggatg gaatcctccg gaggctaccc gtaaaccgat ccctaaagta accagagcca
      781 ggctcaccat ggaagactgg caaaaaattt acaatgcaac gcctgaaaaa cactttatcc
      841 gtaacgcaat gcttcttgcg attgttactg gtcagcgccg tgatgacatt tgccacatgc
      901 gtttttcaga tgtgtggaac gaacacttgc atatcaccca gggaaaaacc ggaatgcgtc
      961 tggcgttacc gcttacacta cgctgtgatg ccattgggat aacgttaaaa gaagttattg
     1021 atgggtgccg agacagaata ttaagtccat atctaatcca tagtcggcac cagaaacaac
     1081 cgaagccgat gagtaaagac aacctgagcg actactttgc caaagcacgg gatctggctg
     1141 ggataattcc accagcagga aaaactccgc caacatttca tgaacaacgc tctctatcag
     1201 aacggctgta ccgtgcacag ggtatcgata caaaaacatt actaggacat aaagtccagg
     1261 caaccaccga tcgctataac gatactcgag gtcaggaatg ggtttaagct ggttatttga
     1321 caaacaccat atggaatatc cgtcaccatc tcaggaaaaa agtgttgtaa aatgcgtggc
     1381 ctagttttgg ggaaacgttt tggagagatt ttggagaaag gaaaaaattg aataaattca
     1441 aaatcctgaa agtgctccac cttatgaata agaaggtgga actcgaatga tcggtcgact
     1501 gcttcgcggc ggttttatga ccgctatcta cgcgtacctg tatatcccaa tcattatttt
     1561 gattgtgaac tcctttaaca gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa
     1621 atggtatagc ctgctgatga acaacgacag cctgttacag gcagcgcagc attcactaac
     1681 aatggcggtg ttttcggcga cgtttgctac gcttatcggt tcactgacgg cagttgcgct
     1741 gtaccgttat cgctttcgcg gtaagccgtt cgttagcgga atgctgtttg tggtaatgat
     1801 atcaccagat atcgtgatgg caatttcgct gctggtgctg tttatgctgc tgggtattca
     1861 gcttggcttc tggtcgctgc tgttctcgca tatcaccttc tgcctgccat ttgtggtggt
     1921 gacggtgtat tcgcgcctga aaggttttga cgtgcggatg ctggaagcgg cgaaagatct
     1981 cggtgccagc gaatttacaa ttctgcgaaa aatcatactg ccactggcaa tgccagcggt
     2041 ggcagcgggc tgggtgttaa gctttaccct gtcgatggac gatgtggtag tttcttcgtt
     2101 cgtcaccgga ccaagttatg aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg
     2161 cgtatcgccg gaagttaacg cgctggcaac catattactg gtgctgtcgc tggtgatggt
     2221 tattgccagt cagcttattg ctcgtgataa aacgaaaggt aacacagggg acgttaaatg
     2281 aaaaaatggt cacgccacct gctcgcggcg ggtgctctgg cactgggcat gagcgccgct
     2341 cacgccgatg acaacaacac gctgtatttc tacaactgga ccgagtacgt gccgccagga
     2401 ctgcttgaac agttcaccaa agaaacctgt attaaggtta tctattcgac ttacgagtcg
     2461 aacgaaacca tgtacgccaa gctgaaaacg tacaaagacg gtgcgtatga cctggtggtt
     2521 ccttcaacct attacgtcga taaaatgcgt aaagaaggga tgatccagaa gatcgacaag
     2581 tcgaagttaa cgaacttcag caatctcgat ccagacatgc tcaacaagcc ttttgacccg
     2641 aataacgact actccattcc gtatatctgg ggggcgacgg cgattggtgt taacggtgat
     2701 gcggtggatc cgaaatctgt caccagctgg gccgatctgt ggaagccaga gtacaaaggc
     2761 agcctgctgt tgaccgacga tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc
     2821 tattccggta acaccaccga tccgaaagag attgaagctg catataacga gctgaaaaaa
     2881 ctgatgccaa acgtcgcagc gtttaactcc gataacccgg ctaacccgta catggaaggc
     2941 gaagttaacc tcggcatgat ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg
     3001 cctattgacg tggtgtggcc gaaagaaggc ggcatattct ggatggacag cctggcgatc
     3061 ccggcaaatg ccaaaaacaa agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg
     3121 gatgtggcaa aacaggttgc tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt
     3181 aagctgttaa gtccagaagt ggcgaacgat aaaacgctct acccggatgc tgaaaccatt
     3241 aaaaatggcg aatggcagaa tgacgttggc gcagccagca gcatttatga agagtattat
     3301 cagaagctga aagcaggacg ttaattcagc aagcgggcgg tggtattacc gcccgttttt
     3361 tatttagcaa ggatgtggct aatgagtcag gacagtagag ttttatccga ctctttcgtc
     3421 ggtattgtgg tccgctcgta ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt
     3481 tataatccct tcagcaactt ttcaacaaac tcaggcacca cctggcttgc cgggccgtaa
     3541 tatttctcgg caaattcatt accgacctga cttggttcga gattcagttc cacggtgtgc
     3601 gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg gataaacatg cccggaagta
     3661 ccaatggcaa tgaaaatatc agccatcgac aacgccatat aaatttcatc catgccgagt
     3721 ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg
     3781 catttatctt ctggcgtaac gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa
     3841 cgaactttca gcaattcccc atgcatatga atgacattgg tattacctgc gcgttcatgc
     3901 aggttgtcta tattctgcgt taccagcaaa aagcgatcgc cgagagcctc ttgcagttta
     3961 gccagcgcaa gatgcgcggc gttaggctga atttttggct gctgcagctg tcgacgacgg
     4021 gcgttataaa acgcttgcac cagttcagga tcgcgatcga aaccttccgg cgttgccaca
     4081 tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc gaaaggtacg aatacctgat
     4141 tccgcagaaa ttcctgcccc tgtcagtacg agtactcttg gtttttccat tgcttccggc
     4201 accactttat ctctgaaaaa aatacgctga cgcaaacgct cgcgcaggcg gcgtttattt
     4261 ttacgaaaac gacttaaccg atgaccccga cgcgacagca tagcaacctc tgttgtttaa
     4321 tcggttagat gtaggaaggc cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt
     4381 tcaatgcgcg gaacacgagc tacaggtaag agatgacgag gcagcctgtc cgccagttgc
     4441 gttgtgattg ccgggaaatt cgataagcca ccaccaatga cgaccaggtc agggtcaaca
     4501 atggtcagga tatttcccag acaaaccgct aataaatcca gataacgctc aacgtgcgcc
     4561 cttgcctgct catcgccttg attataaagc gcaataattt cgggagcctg caactgttga
     4621 tgataatagt gttgatacag ccacgcaaaa ccgcgaccag acagataatt ctcaatgcag
     4681 ccatgctgac cacagccgca gcggcgtaac gggaaatcca accccatcat ggttaacgca
     4741 tcaaccggca gacgcatatg gccaaactcg ccggtaatgt agcttttacc ggtaatcggt
     4801 ttgccgttga aaatcagccc accgccaacg ccggtaccaa gaatcaaccc catcaccagt
     4861 ggatattgcg taaattcatc atcccaggct tctgaaaggg caaaacagtt ggcatcgtta
     4921 tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg cacgcagcgg tttaccgctg
     4981 gcagcgggga cattggcggc atacagcgta ccatcttctg tttccggcat tcccggtata
     5041 ccaatgccga cagaacctct acagccaaaa cgttgatcag cttcggctac cagctcgcac
     5101 actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg gcacccgctt ttcccactgc
     5161 aactgccgac cgctatcaaa cacgccaagc gcaatttttg ttccaccaat atcaaacccg
     5221 taatacattg ccgctccttg ttttaatgta ctgcctttac tggccgctaa ggactcgcgc
     5281 agggtcaata ttactggcgc gccgcgccgg ataccaactt gccaaaagac tcagcaacaa
     5341 tgctgtgacc agtacgtaga agacgtccag ccagtgcaat tccgatggca ggaagtcaat
     5401 aaaatagata tcgctggaga ggaactgatg accgatcagc ttttcaatcc gctcaataat
     5461 cggggtaagt tgcagtgaaa caactacgcc gataatcagc ccacacaggc tgccgaacag
     5521 ccctgccagc aatccatacc agacaaagat ggcgcgaatt aaaccatctt tcgcccccag
     5581 cgttcttaat actgcgatat cgccactctt atctttcacc gccatcacta aggtggagac
     5641 gatgttgaaa caggccacgc caatcaccag taccatcgcc agatacataa tggcgcggat
     5701 catctggata tcgcgataca tatagccgta agtaccaatc cagcttttaa tataaacata
     5761 gctgttggtc atttcacccg catcgcgtac cagcttattg gcgttgaaaa catccgtcat
     5821 tttaagggca atacctgaca cgctggaacc catatcaaga tattgttggg catccgccag
     5881 cgggatcatg gcaaaactgt gatcgagttg gccactcaac tgcaaaatac ccgcaacgtg
     5941 caaacgcaca cgttttggct gcatcagttt atgctcagga ttcgagttgg ggatcatgat
     6001 cgacacccaa tcgccctgct tcactttcag cgcatccgcc acgcctttgc cgatgataat
     6061 ttgctgttcg cccgctttaa aattgcgcca cgcatccccc tgaacaaacg agggtaatgc
     6121 gctcagacgc tgttcctgtt gtgggttaac gcccttcacc tggattgcgc gcagattcgc
     6181 tccactttcc accagcccgg tgaaattgat atacggcgca gcggcggcaa tacccggcac
     6241 tttctgcact ttatccagtg cttcctgcca gttagtccat ggctgattta ccgcttcgat
     6301 ttcaccatgc ggcaccaccg ccagaatgcg gttattcagt tcgcgttcaa agccgttcat
     6361 cgcgcttaag ccgacgatca ataccgccac accgagggca atgccaatgg tagaaatgac
     6421 ggagatcagc gacaccatgc cgccgcgccg ccgtccgcga ctaaaacgca ggccaattaa
     6481 taacgataaa ggcatcgcca ttactccgcc cccatcaggc tcagttccgc cgtcagacgt
     6541 ccatcacgca tttctaactg gcggctcata cgtttcgcca gttgcaggtc gtgagtaacc
     6601 accaggaagg cggtgccctg caagcgattc aattccccaa gcaactggaa gatgctgtcg
     6661 gcgttacgcg catcgaggtt accggtaggc tcatccgcca gtaccagacg cggattattg
     6721 accagcgcac gggcaatcgc tacacgctgg cgttcgccgc cagaaagttc agatgggcgg
     6781 tgattcgcac gatgctccag ccccaccgct tttaacatct ccagtgcacg gctgttgatt
     6841 tcagcgggct ttttcttgcc aatcagcagc ggcatagcca cgttttccag ggcggtaaaa
     6901 tccggcagca ggtggtgaaa ctgataaata aagcccagtt tctggttgcg cagttctgct
     6961 tttgctgccg acgacagttt gctcatcggt tgaccgttaa agatcacatc gccggaggtt
     7021 ggcgtatcca gtccgcccag caggtgcagc aaggtgcttt taccggaacc agagctaccg
     7081 acgatcgcca tcatttcacc ttcgccgacg ctgaaactga cattgtgcag tacatcggtt
     7141 tgcacactgc cttcctgata gcgtttgcac aggttgtcgc attgcaacag gatcttattc
     7201 ataacgtaaa gcctcagcgg gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga
     7261 cagcagcgcg atagccatcg ccaccagcgc aataacaatg acctgtaaag gttcgatagc
     7321 catcggcagc gccgcgccat caagcaggac gccgattatt ggcatcagat tatttaactg
     7381 gctggcaagc agtgcgccaa gcgccgctcc gaggatcgca ccgataatcc cggcgctggc
     7441 cccttgcacc ataaagatca tcatgatttg tcgcggagtt aagccttgcg tttgcaggat
     7501 cgctacttcg ccctgcttct ccatcaccat cagccccagc gaggtaataa tgttaaacgc
     7561 cgcaacggcg acaatcaggc tcagcagtaa acccatcata tttttttcca tgcgtacggc
     7621 ctggaacaac tcgcctttac gatcacgcca gtcctgccat ttgctgtctt caggcagttt
     7681 ttgctgactt aatgagtcga ctttcagcgg ctcatccagc cacaaacgcc agccggtaat
     7741 attgcctgcc ggataacgca tcagacgcga ggcatcctca atattcacca gcatttcatt
     7801 gccatcgact tcactgttgg cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg
     7861 aatacgcccc atcggcgtga actggctggc agatggtacc atcatgcgga tttgatcacc
     7921 gcgattaacg cctagctgtg aggcaagttg ttcgccgagg atgacattat atttccccgg
     7981 ctcgaggtca gtttgtttca cattgaccag atacggcgtt agtggatctt tttgcgccgg
     8041 atcgataccc agcatcaccc cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc
     8101 ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg actgccgttt ccgggagttg
     8161 ctgcgggtta agagagccat gctcagaaga gagaattgcc tgtggcatca ggccaaggat
     8221 gttgttttgc agctcgcgct caaagccgtt catcactgac aatactgtga ccagcgccat
     8281 caccccgagg gtaatgccga tggtagaaag ccaggagacg aaacgaccga agcgatccgc
     8341 tgcacgccca cgcatgtaac gcaggccaat aaatagagcg acaggttggt acatgaaatc
     8401 cgtctggttg ctgtagcaaa gacacgagta tataagcgaa agcaagatgg gtaaacgtct
     8461 gaactgtaag tgatcgtcag ggctaatctg aaaagccccc gtaaatcaat cggctaaatt
     8521 gccatgtttt atcatcaatc ttgccgcctg atcttaatct gaaaaatcat ccaatacaga
     8581 cagataccca ttacacctcg ggtcataacc cgcaggatca accgcatgta aattaaaaaa
     8641 gagcgtgttg tcctgatgaa acaaaaagag ctatggatta accagatcaa agggttatgt
     8701 atttgtccgg tggtgattta tcactcggtc attacctttt atccgcatat gaccactttt
     8761 ccgcatccgt tatcggaagt cctgagcaaa tgctggatct atttcaatct ttaccttgca
     8821 ccctttcgta tgccggtttt tttctttata tccggctatt tgattcgccg ctatatcgac
     8881 agcgtaccgt ggggaaattg tctcgataaa cggatctgga acatcttctg ggtgctggcg
     8941 ctttggggcg tggtgcagtg gctggcgtta agtgcgctga accagtggct ggcacctgag
     9001 cgcgatttaa gcaatgcctc caatgccgct tatgccgatt ccaccggtga gttcctgcac
     9061 gggatgatca ccgccagcac cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg
     9121 gtatgtaaaa tttttagccg tctggcgctg ccactattcg ccttgtttgt actgctgagt
     9181 gtggcggtta atttcgttcc cacgccgtgg tggggaatga acagtgtgat ccgcaatttg
     9241 ccttattaca gccttggcgc atggtttggc gcaacaataa tgacctgtgt taaagaggta
     9301 ccgttgcgcc gccatctgct gatggcttct ttgcttaccg ttctggcggt cggtgcctgg
     9361 ttgtttacta tctcgctgct gttgtcgctg gtatcgattg tggtgatcat gaagctgttt
     9421 tatcagtacg agcaacgatt cggtatgcgc tccaccagcc tgctgaatgt gattggctcc
     9481 aacaccattg ctatctacac cacccatcgc attctggttg aaatattcag cttaactctg
     9541 cttgcgcaaa tgaacgcagc acgctggtcg ccgcaagtcg aactgacact cctgctggtt
     9601 tatccctttg tcagtttgtt catctgtact gttgcgggct tgctggtaag aaaactttca
     9661 cagcgtgcat tcagcgatct gttgttctcc ccgccttcac tgcccgctgc cgtcagttac
     9721 tcccgctaag ccatcgcccc agccggggcg attcaatttg ctaaaccatg tcgattacgg
     9781 ataatgaaca gcattgctta tcagagatat gcccccatat gttgaggcat atcctaacga
     9841 gaatctgaca accgttatgc ctgaacaata tcgttatacg ctgcccgtca aagcgggtga
     9901 gcagcgtctg ctgggcgagt taaccggcgc agcctgtgca acgctggtag cggaaattgc
     9961 cgaacgtcac gccggtccgg tggtactcat tgcaccagat atgcaaaatg ctctgcgttt
    10021 gcatgatgaa atcagtcagt tcaccgatca gatggtgatg aatctggcgg actgggaaac
    10081 tcttccctac gacagctttt cgcctcatca ggacattatc tcctcgcgcc tttccaccct
    10141 ttaccagcta ccgacgatgc agcgtggcgt actgattgtt ccggtgaata cgcttatgca
    10201 gcgcgtttgc ccacacagtt ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg
    10261 cctgtcacga gatgcattac gaacccaact ggacagcgcc ggttatcgcc atgttgacca
    10321 ggtgatggag cacggcgaat acgccacgcg cggcgcgttg ctggatctct tcccgatggg
    10381 gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt
    10441 tgacgtcgac agccagcgca cgctggagga agtagaagcg atcaatctgc tgcccgcgca
    10501 cgaatttccg accgataaag cggcaattga actgttccgc agccagtggc gcgatacctt
    10561 cgaagtgaag cgcgatccgg aacatattta ccagcaagtg agtaaaggca cattacctgc
    10621 cgggatcgag tactggcagc cattgttctt cagcgaacca ctgccgccgc tgttcagtta
    10681 tttccctgcc aataccttgc tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt
    10741 ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt
    10801 gccaccacaa tcgctctggc tgcgggtgga cgagctcttc tcagagctga aaaactggcc
    10861 ccgggtgcag ctaaaaacta aacatttacc gacaaaagcc gcgaatgcca atttaggttt
    10921 ccagaaactg ccagacctag ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg
    10981 taagttcctc gagactttcg acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg
    11041 tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct
    11101 tgatgaagcc agcgaccgtg ggcgttatct gatgattggc gctgccgaac atggttttgt
    11161 cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc
    11221 ccgccgtcgt caggattctc gccgcaccat caaccccgat acactgatcc gtaaccttgc
    11281 ggagctgcat attggtcagc cggtggtcca tctggagcac ggtgtcgggc gctatgccgg
    11341 aatgaccacg ctcgaagcgg gcggcattac tggcgagtat ttgatgctca cctatgccaa
    11401 cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg attagccgtt acgcaggtgg
    11461 cgcggaagaa aacgccccgc tgcataaact tggcggcgat gcgtggtcac gcgcgcggca
    11521 gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg ctggatatct acgcgcaacg
    11581 cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag cagtatcagt tgttctgcga
    11641 cagcttcccg tttgaaacca cgccggatca ggcgcaggcc attaatgcgg tacttagcga
    11701 catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac
    11761 agaagtggcg atgcgcgccg ctttcctggc agtagataac cacaagcagg tggcggtgtt
    11821 ggtgcctacc acccttctcg cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa
    11881 ctggccggta cgtatcgaaa tgatctcccg tttccgcagc gccaaagagc agacgcaaat
    11941 ccttgcggaa gtggcggaag ggaaaatcga tattctgatc ggtacgcaca aactgctgca
    12001 aagtgacgtc aagtttaaag atttaggcct gctgattgtc gatgaagaac accgcttcgg
    12061 ggtgcgtcat aaagagcgca ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac
    12121 tgcaacgccg atcccacgca cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat
    12181 tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc tttgtccgtg agtatgacag
    12241 cctggtggtc cgggaggcga tcctgcgtga aattttgcgc ggggggcagg tttattatct
    12301 ctacaatgat gtggaaaaca tccagaaagc tgccgaacgg ctggcagaac tggtgccaga
    12361 agcgcggatc gccatcggtc acgggcagat gcgcgagcgc gaactggaac gggtgatgaa
    12421 tgatttccat catcaacgtt tcaacgtgct ggtttgtaca accattatcg aaaccgggat
    12481 cgacatcccg acagccaaca ccattatcat tgaacgcgcg gatcacttcg gtctggcgca
    12541 gctgcaccag ttacgcggtc gcgtcggacg ttcacatcat caggcatatg catggctgct
    12601 gacgccgcat ccaaaagcga tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc
    12661 gctggaagat ctcggtgcag gctttgcgct ggcaacgcac gatctggaga tccgcggcgc
    12721 gggtgaactg cttggcgaag aacaaagtgg ctcaatggaa accatcggtt tctcgctgta
    12781 tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga
    12841 agatctcacc agccagcaaa cagaagtcga gctgcggatg ccgtcgctat tgccagatga
    12901 tttcattcct gacgtgaata cgcgtttgtc gttctataaa cgtattgcca gcgccaaaac
    12961 ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat cgcttcggcc tgctgccgga
    13021 tccggcgcgt accctgctgg atgttgcccg tctgcgccag caagcgcaga aactggggat
    13081 caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt
    13141 taatccggcc tggttgattg gtttgctgca aaaacagccg cagcattacc gccttgatgg
    13201 tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt
    13261 acgccagttt atgcgtgaac tggaagagaa cgcgatcgct taatggtaaa tcgggggcgt
    13321 ttctgcgccc ccatcggcaa catttacaaa ttctttgcac ttccctgcac tatccgacac
    13381 tgtcaccatc cataattcag gcttatctgt ttattacaat aaccttatat ttattatgga
    13441 tttttggtga tgatcaaaac gcgtttttct cgctggctaa cgttttttac gttcgccgct
    13501 gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc cgctgccgcc agcgggcagt
    13561 cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc tctggaagcc
    13621 atcgccaaaa aatataacgt cggctttctc gctctgttac aggctaaccc cggcgttgat
    13681 ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt tgcaaaccct acttccagac
    13741 gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc gtctctatta ctacccgccg
    13801 ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc agttaggtgg tgacacgctg
    13861 acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa acccaacctg gacgccaacg
    13921 gcaaacaccc gcgcccgtta taaagcacag ggaattgagt tgcctgcggt agtgccggct
    13981 ggaccggata acccaatggg ccatcatgcg attcgtctgg cggcctatgg cggcgtttat
    14041 ttgcttcatg gtacgaacgc cgatttcggc attggcatgc gggtaagttc tggctgtatt
    14101 cgtctgcggg atgacgatat caaaacactc tttagccagg tcaccccagg caccaaagtg
    14161 aatatcatca acactccgat aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa
    14221 gtacatcagc cgctgtcaga gaagattgat gacgatccgc agctgctgcc aattacgctg
    14281 aatagcgcaa tgcaatcatt taaagatgca gcacaaactg acgctgaagt gatgcaacat
    14341 gtgatggatg tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa
    14401 actctgtaaa tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc
    14461 agcagtagca ggcattaatg agggttaatg cttatttata aattactgct gttccatgga
    14521 gggtattcgg accggttaca gaagtaatac ggaaagattt tgcacccatc tcatccgctt
    14581 tttgcgccag ctgctcttcc agcgatccca gatttgtccc cgctttagca ctgattgtac
    14641 cgactttttg ttggccttct ggcgttgact gaacttcgac agccgcaaag ctggcaaatg
    14701 acatggagct taaaatcgcc gcagcgatga gggtttttac gtttttcata atagtggcct
    14761 tatgcagatg aatgacgtcg caagcaatta cttaacgatc gataactaaa tgacagatgt
    14821 gatctggatc acatacaaga tattttttat aacaatcatt aattaattta aaaatcttta
    14881 aatttcagtt aaatagaaag caattatttt tatgggatct ggactggtga acggaggtga
    14941 ttatttttat aataatcttt cattcacagt caccaggtac aacggcatca tggcaactga
    15001 ctcaacacaa tgtgtaaaaa aaagccgtgg ccgcccaaaa gtgttcgaca gggatgccgc
    15061 gcttgataag gccatgaaat tgttctggca acacggttat gaagcgactt ctcttgcgga
    15121 cctcgtcgaa gcgaccggag ccaaagcgcc cacgctatac gcggaattta ccaacaaaga
    15181 ggggttattt cgtgccgttc tcgaccgcta tatcgatcgt tttgccgcta agcatgaagc
    15241 acagctgttt tgtgaagaga aaagcgtgga gtctgcgctg gctgactatt ttgctgccat
    15301 cgccaactgc tttaccagca aagacacccc ggcaggctgc ttcatgatca acaactgcac
    15361 caccctctcc ccagattcag gagatatcgc caatacgttg aaatcacgcc atgcgatgca
    15421 agagcgcact ttgcagcagt ttttatgtca acgacaagcg cgcggggaaa tcccgaccca
    15481 ctgtgacgtg acacatctgg cagaattcct taattgtatt attcagggga tgtcgatcag
    15541 cgcacgcgaa ggtgcatcgc tggaaaaact aatgcagatt gccggaacga ctttgcgttt
    15601 atggcccgaa ctggtgaaat aagtcgcaaa aaaagcccct cagcctgagc gaatgagggg
    15661 ccaaattgca gagtacagcc ttgttatact ttgttattca aaatcagttg cccattacta
    15721 tctagcggga tctgcgtacc cggatcgcgg tccatgcgga ttttgccctg ctgatcgcca
    15781 atcttatagg tcacatcata accgagcatt ttttctgact tgtcatacac cgttttacaa
    15841 cgctgttgcg tagtcgtgta agtatcgctt tcctggagag agccctggat ctggttaccg
    15901 gcatatccac cacccagcgc ccccacaaca gtggcgacat ctttaccgcg accaccgcca
    15961 aactgatgcc ctatcacgcc gccagcaaca gcgccgagca ccgacccggt aatgcgattt
    16021 tcatcctgca ccggtcgacg atgggtcact gtgacgttgc gacactcctg acgcggtgtt
    16081 ttaaccgttt ccttgattgg ggttgcagaa acaacctgag cgtattgcgg gccacgttca
    16141 aacacgttca gactggccac tgccgctacg cccagcgcag ctgcgacacc aatcccgata
    16201 cccgccaaca ttgatttatt cacgggaaca cctccttctt tgcagttatg ccaatcatgc
    16261 aagcgaagga gaaaagcagc tatcagacag aagatgaaaa gcgcgggaat ggctggaaat
    16321 acttaatgat tcggaggagt ctgaaggaat gcctgatgcg acgcttaacg cgtcttatca
    16381 ggcctaccgc tcgtaccatt tgtccagggc ggatgaggcg tttatgccac atccgccagt
    16441 gtacgtcgat taatgcaact tcaaacgcgg acggataacg cggttaatac tccccaccag
    16501 catcattaat ccggttttaa agtaaccatg cagcgcaatc tgatgcattc ggtatagcga
    16561 gatatataca aagcgcgcaa ttcgtccttc aatcatcatt gagccgcgcg tcaagttacc
    16621 catcaggcta ccaacggtgg agaagttcga cagcgatacc agtgaaccat gatctttata
    16681 ctgataattt ttcagcggct taccgtttat ctgcgccaga atattgttca ttgcgcaagt
    16741 cgccatctgg tgtgcagcct gagcacgcgg tggaacaaag cccccttccg gacgcgtgca
    16801 tgacgcgcag tcgccaatag cgtaaatgtc tggatcgtgg gtggtttgca gcgtcggttc
    16861 caccaccagc tggttgatac ggttagtttc aagaccaccg atatctttca ggaagtctgg
    16921 cgctttgatc ccggctgccc acaccatcag atcagcctca atatattcac catctttagt
    16981 gtgcaggccg ccttcatcag cactggtgac catggtttgc gtcagcacgc gaacgccaag
    17041 tttcgttaac tcactatggg ccgcagcaga gatgcgtggc ggtaatgcag gcaaaatacg
    17101 ttctcccgct tctactagcg ttacgttcag ggcttcgttg gtcagacctt tataaccgta
    17161 gctgtgcagt tgcttgaccg cgttatgcaa ctcagcggag agttctacac ccgtcgcgcc
    17221 gccaccgaca atcgcaatgt tcactttgcc attcgcgccc aggttggcgg agtatttcag
    17281 gaacagattc agcatctcct ggtggaagcg acgcgcctgg tgcgggttat cgaggaaaat
    17341 gcagttctct ttgacgcctg gcgtattgaa atcgttagag gtgctaccca gcgccatgac
    17401 cagggtgtca taggcgattt tacgttccgg aaccagcagt tcacctttct cgtcgcgtag
    17461 ttctgcaata gtgattgttt tcgcttcacg atcaatatca atgacggaac ccaactggaa
    17521 ctggaaacca tgattgcgcg catgggccag atagctcaac gcatcgacgc cttcatcaag
    17581 cgagccagtc gccacttcgt gcagcagcgg tttccacagg tggctgtggt tacgatcgac
    17641 cagcgtaatt ttggcttttt tcttacgtcc cagcttatgc cccagctgtg ttgccatttc
    17701 cagcccacca gcaccgccgc cgacaatcac aatctttttc aatggcgtag tcaacgtgac
    17761 ccccttgaat ttattaacca attgttaatt aatggttatt aacatagctc ttaattaaca
    17821 acaggttaca gcaatgaaaa tgttcttcgc tattgagaat aacatgaatg gtgcattggt
    17881 cataccaaaa ttgatgttaa tcaagttttg ttgctgaaaa gataggcgac tttaccgggt
    17941 gagatcaaaa aatagagttt attgtttatt atttgagcga atttgtgaga gtgatataga
    18001 gtgaggggtg tatgcagggc gttcggtagg cctgataaga ggcgcatcag gcattgatgc
    18061 tcgactgtcg gatgcggcgt aaacgcctta tccgacttac agaattcgga gcgcatcagg
    18121 taccggcaag gttacctgat gcgacgaggg catttacccg agggttttga acgctttaat
    18181 gcgctgcaaa tgcggggaga tattcttgaa tttgtgcgtc tgctcttcgt cccagacaat
    18241 ctcgtaataa tgatgcaact cttcagatgt ccgctggctg ttaagcgctt catcattacg
    18301 cgacaaaatc accaggcaac gatcgcgatt cttctcgcgg aagttggtca cacacttagt
    18361 ggcaatatcg gcatactctt ccgggcgatc aatcttgcct tccatgttct cataagggaa
    18421 caaattaggg ttgaagatca cctggcggat atcgcaaaga aaaccaatcc gttccgccca
    18481 gtatccgccc agaccaacgc cgcaaatcag cggacgctcg tcgacgttca gttgcaacat
    18541 tttgtccact tctttaagca gatgctgcat atcatgtttc ggatgccgcg tactgtagct
    18601 tatcaagcgt acatccgggt caataaactg caattgtaag actttctcgt ggttacccgg
    18661 actgttagag tcaaaaccgt gtaaatagat aatcatcgct tcctcacata agccagggtt
    18721 agtgacctgc tttctcttcc tgccagcgtt catgtaactg attcagacgg gtgctgctcg
    18781 ccttccagcg agccgagtcc atcagttcct gtcgcgaaaa tgaaccttta tgatacaaac
    18841 gtgtaacacg ttctgccttg atcggtgaca gattatctaa cacgctgacg gcccctttac
    18901 gattattgca gaccaggatc atatcgcaac ctgcgtccag tgatgcctgc ccgcgttcgg
    18961 cataactgcc cataatcgcg gcaccttcca tcgataaatc gtcagagaaa attacaccgt
    19021 caaaacacag ttcctgacgc aaaacggttt tcagccagta agaagaaccg ctcgccggac
    19081 gcggatcaac atcactgtag atcacatgcg caggcataat ggcgtcgagt ttattttcgc
    19141 ggattaacgt gctgaaaacc gacatatctt tggcacgaat ttccgcttgc gggcgtgggt
    19201 cgcacggcgt ctctttgtgt gaatctgcag ttactgcacc gtgtcctggg aagtgtttcc
    19261 cggtcgtttt cattccggct tcatgcatcc catcaataaa ccgactagcg attgccaggg
    19321 ctttttctgg gtcggcatga taagaacgct cgccaatcgc cgcgctgata tgtcctacat
    19381 ccagcactgg cgcaaagctg atatcaatat ccatagcgat catttcgctg gccatcagcc
    19441 aacccgcctc ttgcgccagt ttgccgccct cttccattcc caacagcgca gcaaaggatt
    19501 gtgctgccgg taagcgggta aaaccttcgc gaaaacgctg cacgcgtcca ccttcctgat
    19561 ctaccgccac caccagatga ttgcgcgatg ctgcgcggat ctggcgcacc agttcacgta
    19621 actgggcagg atcatgatag ttacgcgtaa agagaatcag ccctcccacc agcggatgcg
    19681 ccagtatttc acgctcttcc gcgtccagtt cataaccttc gacatccaac attactggac
    19741 ccacactgct ctccttattg ttttattaat agctgccgcc aggtgtcatc ggccagcctg
    19801 ataaattgtt gatcgccggt ttgtcgccag cggtactcaa accaccctgc tttgagcatc
    19861 agcagccagg gaaaccatcg cctgacctga cgccataatt gcgccggata aatcttcgcg
    19921 cgagtggcat agtcattgac caattgccgg tgctgttcag tattttccac ccacaccgcc
    19981 gccagttcca gcgcggtatc accatctccg gcatactccc agtcgatgag ttttaaccct
    20041 gatgcgctat gcactaaatt tccggcgtgg acatccatat gcaatggact taagcgtaaa
    20101 ggtcgtggtt cccgcgcttt gcgcagacgt tttaacattc gcagccaacc cacagtccgc
    20161 cgcgccggat cgctttgctg ccagtacagt tccagtaacg gcaacagcgt tattcgccag
    20221 ccaaaacgtg gttgttgatg tagataatac agcaagcctg ccagttcgtt ggtatccggc
    20281 aaatacgttt ttaccgcgcc gggcagatag tcgactacca tccagtcacg gagatataaa
    20341 tgcggcttcg gtgcaatgct tgcgggtagt tgtgataaag cccgatactg gcgcaagaac
    20401 gcggactgcg gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt
    20461 tcaatgagaa aactaccgcc actaagccca ctattaaacg tcgtgacggg atgaaactgc
    20521 gggaaaaagc gcgacagcaa ttcgtcgcgc gtgatgggat tattactgcg aaacggcacc
    20581 tttacctgac cagataattt cgcccgtctg caccagcatc agctgcattt gtagggtcgg
    20641 agcgttaacg ttgccagagg cgctggagta cagcacgtaa tgagcgccga cattgcgggc
    20701 aatgcctatg gctttactac gggtgcctaa actgtcctgc ggcgacaaac ctaactgttg
    20761 cttagccatc gacagttgct gggcggaaac cagggtaaat ttcccgttat tagccagcgc
    20821 atttcgcagc gtttcggtcg cttctgcggc attcagcgaa ccgttagtac ggttgttaac
    20881 gctatcaacc agcaggacgc tacccgcagt caccccgtca gccccaagca tcttactgac
    20941 tatcggctgc attgcgccat tccagtcata atggcgaata tgcggcgcag gcggtgccgt
    21001 tcgatcttcg tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg agggcactgt
    21061 ggggacaggc tgttgtggct cggctggttg ttccggcgct ggtttcactt cttctaccgg
    21121 tgcaggttca cgttgcccca cacacccggc gagaaacatc gccagcgcgg taatcaaggc
    21181 gtagcgactc atttttgtca tcaagattca ccccttacaa atatagataa agtctgactt
    21241 tgtgcgcccc cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg
    21301 taacgctgcg tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa
    21361 aacgataatg aacggtaacg ggtttttgcc tttcgttata aagcgttgag gatgctgaag
    21421 gttgaatatc agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag
    21481 atgactccat caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc
    21541 ccaccagcag taacaacacc agagacacca gcccaaagca tccttttctc atcgttacag
    21601 acctttatgc gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg
    21661 gtaaacctct tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc
    21721 aataccttct tgctcagcaa tttttgccac tacggtgatc atgcgtccca gcgcctgctc
    21781 atgctcagct gagacgtcgt tcacagtcgg aatgaggata ttcggaatga tcagaatatg
    21841 cgttggcgct tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat
    21901 atcggagggg atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt
    21961 ttcctttttc aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag
    22021 agtatggtcg agttaaacct gctctttcaa ccttaactca cgatttctta agcaaaaaat
    22081 cgttttgctg ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag
    22141 acattctctg tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttac
    22201 aaacaaaatt attcgcacac taaatataat cgctcatcac cctcctccgg catgagcctg
    22261 acgacaacat aaaccatgag atttcaaatg ctttcaacac aatttaacag ggataatcaa
    22321 tatcaagcca tcaccaaacc gtcactactt gccggttgca tagcactggc actattacct
    22381 tctgccgctt ttgctgcatc agccactgaa gaaacggtga ttgttgaggg ttcagccaca
    22441 gctc
//
LOCUS       DAJTNN010000057        22240 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTNN010000057 DAJTNN010000000
VERSION     DAJTNN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.057"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1394)
                     /locus_tag="OO150_001894"
     CDS             complement(<1..1394)
                     /locus_tag="OO150_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HCR5516061.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGMLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGTMLGG"
     gene            complement(1391..1705)
                     /locus_tag="OO150_001895"
     CDS             complement(1391..1705)
                     /locus_tag="OO150_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5516062.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(1837..3796)
                     /gene="acs"
                     /locus_tag="OO150_001896"
                     /pseudo
     CDS             complement(1837..3796)
                     /gene="acs"
                     /locus_tag="OO150_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4189..5625
                     /gene="nrfA"
                     /locus_tag="OO150_001897"
     CDS             4189..5625
                     /gene="nrfA"
                     /locus_tag="OO150_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5516063.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5670..6236
                     /gene="nrfB"
                     /locus_tag="OO150_001898"
     CDS             5670..6236
                     /gene="nrfB"
                     /locus_tag="OO150_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5516064.1"
                     /translation="MSVLRSLLTARVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6233..6904
                     /gene="nrfC"
                     /locus_tag="OO150_001899"
     CDS             6233..6904
                     /gene="nrfC"
                     /locus_tag="OO150_001899"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5516065.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            6901..7857
                     /gene="nrfD"
                     /locus_tag="OO150_001900"
     CDS             6901..7857
                     /gene="nrfD"
                     /locus_tag="OO150_001900"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5516066.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLAQQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            7877..9595
                     /locus_tag="OO150_001901"
     CDS             7877..9595
                     /locus_tag="OO150_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5516067.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9588..9971
                     /gene="nrfF"
                     /locus_tag="OO150_001902"
     CDS             9588..9971
                     /gene="nrfF"
                     /locus_tag="OO150_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5516068.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            9968..10564
                     /gene="nrfG"
                     /locus_tag="OO150_001903"
     CDS             9968..10564
                     /gene="nrfG"
                     /locus_tag="OO150_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5516069.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYVALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            10905..12218
                     /gene="gltP"
                     /locus_tag="OO150_001904"
     CDS             10905..12218
                     /gene="gltP"
                     /locus_tag="OO150_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5516070.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12409..13097)
                     /locus_tag="OO150_001905"
                     /pseudo
     CDS             complement(12409..13097)
                     /locus_tag="OO150_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(13191..15338)
                     /gene="fdhF"
                     /locus_tag="OO150_001906"
     CDS             complement(13191..15338)
                     /gene="fdhF"
                     /locus_tag="OO150_001906"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(14919..14921),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5516071.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIMANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15536..17002)
                     /gene="mdtP"
                     /locus_tag="OO150_001907"
     CDS             complement(15536..17002)
                     /gene="mdtP"
                     /locus_tag="OO150_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5516072.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGIAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(16999..19050)
                     /gene="mdtO"
                     /locus_tag="OO150_001908"
     CDS             complement(16999..19050)
                     /gene="mdtO"
                     /locus_tag="OO150_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5516073.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19050..20081)
                     /gene="mdtN"
                     /locus_tag="OO150_001909"
     CDS             complement(19050..20081)
                     /gene="mdtN"
                     /locus_tag="OO150_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5516074.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDVLVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPNDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20100..20375)
                     /locus_tag="OO150_001910"
     CDS             complement(20100..20375)
                     /locus_tag="OO150_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516075.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20584..>22240)
                     /gene="yjcS"
                     /locus_tag="OO150_001911"
     CDS             complement(20584..>22240)
                     /gene="yjcS"
                     /locus_tag="OO150_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR5516076.1"
                     /translation="QLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTP
                     PSAKAALDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFM
                     DEAISENVLAGNIMSHRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITK
                     TGEKMIIDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTR
                     DTSKWTEYLNETLDMWGNDAEVLFMPHTWPVRGNKHINDYIGKYRDTIKYIHDQTLHL
                     ANQGYTMNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPY
                     GQVEMGKRYVQALGGSARVISLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQAN
                     NFEQLGYQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVR
                     LDSAKAAGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLT
                     GQAKMADLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         4982 a         5861 c         6078 g         5319 t
ORIGIN      
        1 ccgcccagca tcgtgccacg ggtagtcagt ttcgaccagt acatggaaag cagaatgatc
       61 gggaagttac agctcgccgc gatggcaaac gcaaggccta ccataaaggc gatgttctga
      121 ttctcaaaca gcatgccgag gataatcgca atcacaccca aaatcaggac ggtgattttc
      181 gatacccgca gttcttcacg ttcggtcgca ccttttttga agacgttagc gtacaagtca
      241 tgcgaaaccg cggatgcgcc cgccagcgtc agacccgcaa ccaccgcgag gatagtggcg
      301 aaagcaaccg ctgaaataaa accgaggaac aggttgccgc ccactgcatt cgccaggtga
      361 acggccgcca tgttgttacc accaatcaga tggcccgccg cgtctttata ttccggattc
      421 gcaccaacca gcatgatcgc gccgaagccg ataataaagg tcagaatata gaagtagccc
      481 ataaacccgg tggcgtagaa cacgctctta cgtgcttcgc gggcatcgct gactgtaaag
      541 aagcgcatca gaatgtgcgg caagcccgcc gtaccaaaca tcagtcccag acccagagag
      601 agcgcggaga tcggatcttt caccagcccg cccggcttca tgatgtcgac acctttcggg
      661 tgtaccgcca tcgcttcgct gaacagatta ttgaagctaa agccgacgtg tttcatcacc
      721 ataaaggcca taaagctggc accgaacagc aacagcacgg ctttaataat ttgcacccag
      781 gtggtcgcca gcatgccgcc gaacaggacg tacatcatca tcagcacgcc gaccagcacc
      841 accgcaatgt gatagttaag gccaaacagc agctcgatca gtttacctgc gcctaccatc
      901 tgggcgataa ggtaaagcgc caccaccacc agagaaccac aggccgaaag aatacgaatc
      961 ggcccttgtt tcaggcggta agaggctaca tcggcaaagg tgtagcgtcc caggttacgc
     1021 agacgttcgg caatcaggaa caaaatgatc ggccagccca ccaggaagcc cagcgagtaa
     1081 attaagccgt catagccgga ggtaaacacc agcgcggaga tccccaaaaa tgaggcggcg
     1141 gacatatagt caccggcaat cgccagcccg ttctggaagc ctgtgatatt gccgcctgcc
     1201 gtgtagtagt cgctacgaga acgtacgcgt tttgatgccc agtaggtaat gccgagcgta
     1261 aacacgacga aaatcaggaa cataataatc gcctgccagt tcgttggctg gcgctctacg
     1321 gccccgctaa tagcatccgc ggcgttagct gcgaaaggga gtgtggcggc aagcgccgtc
     1381 agaactcttt tcatgatgct tgtacctcat gcaggacttc attattaaga cggtcgaatt
     1441 cgccgttcgc ccgccagatg tagataccgg tgagaacaaa ggagatcaca atcactccaa
     1501 caccaattgg aataccgcgt gtgacgctgg tgttcggatt cagcggcgtg cccagccagc
     1561 cgggcgcgaa ggcgatcagt aaaataaagc cgatataaac tgccagcata ataatcgaca
     1621 ggatggtggc aaaccgttgc cgtttttcga ctaactccct gaaatgcgca ttgtcttcta
     1681 tccgctgata aatagtgcca ttcatcacag attctccaga ggtaatgtag ggattgtttt
     1741 aattcccgct cccttatggg agaaggttaa cgctcgggta acccttgccg aatgtaggcc
     1801 ggataaggcg tttacgccgc atccggcaat tgtgggttac gatggcatcg cgatagcctg
     1861 cttctcttca agcagcttct cgactacgcc aggatcggca agcgtcgagg tatcgcccag
     1921 gttgctggta tcgcccgccg caattttgcg cagaatacgg cgcataattt tgccggagcg
     1981 ggttttaggc agggagtcgg tccagtgcag cacgtctggc gtcgccagcg ggccaatctc
     2041 tttacgcacc cagttgcgga cttctgcgta cagttctggt gacggttcct ccccgtgatt
     2101 aagcgtgacg taggcgtaga tcgcctgacc tttaatattg tgcggaatac ctactacggc
     2161 ggcttcggca atcttcggat gcgccaccag cgccgactca atctctgccg tccccagacg
     2221 gtgaccggag acgttcagca cgtcgtccac atgcccggtg atccagtaat agccatcttc
     2281 atcgcgacgc gcgccgtcgc cgctgaaata catatttttg aaggtggaga agtaggtctg
     2341 ttcaaaacgt tcgtgatcgc caaacagcgt acgcgcctga cccggccagg agtcggtgat
     2401 caccaggctg ccttcggtag cgccttccag cgggttacct tcgttatcga ccagcgccgg
     2461 ttgcacgccg aagaacggac gtgttgccga accggctttc agcccggtag cgccaggcag
     2521 tggcgtgatc atgaaaccgc cagtttcggt ctgccaccag gtatcgacca ccggacattt
     2581 ctcgttgccg atttttttcc agtaccactc ccacgcttcc gggttaattg gctcgcccac
     2641 ggaaccgaga atgcgcagcg acgaacggtc ggtaccctcg atcgctttat cgccttccgc
     2701 catcagcgcg cggatcgccg tgggtgcggt atagagaata ttgacctgat gcttgtccac
     2761 cacctgcgcc atacgggcag gcgtcggcca gttgggtacg ccttcaaaca tcagcgtggt
     2821 cgcaccgcag gccagcgggc cgtacagcaa gtaactgtgt ccggtcaccc agcccacatc
     2881 ggcggtgcac cagtagatat cacccggatg ataatcaaag acatatttaa aggtcagcgc
     2941 cgcgtacacc agataaccgc cggtagtatg cagcacacct tttggcttac cggtagaacc
     3001 ggaggtgtag agaataaaca gcggatcttc ggcgttcatc tcttccgcct ggtgctgatc
     3061 gctcgcttgc tcaaccaggt cgtgccacca caggtcgcgc ccttcctgcc agtcaatttt
     3121 cccgccagta cgcttcagta ccaccacatg ctctacgctg gtgacgttcg ggtttttcag
     3181 cgcgtcatca acgtttttct tcagcggaat actgccgccc ggcacgcaca ccttcgtcgg
     3241 aagtgatcac cagtcgtgag ttggaatcaa taatgcgccc ggcaacggct tccggcgaga
     3301 agccgccgaa aatcaccgaa tgcaccgcgc caatgcgggc gcaggccagc atcgcaaccg
     3361 cggcttccgg caccatcggc atataaatcg ccaccacatc acctttttta atgcccagct
     3421 cgagcagggt attggcgaag cggcagacgt cgcggtgcag ctctttatag ctgatatgtt
     3481 tgctctggct ggcgtcgtcg ccttcccaga tgatggcggt acgatcgccg ttttcttgca
     3541 gatggcggtc aaggcagttt gccgccagat tcagcgtgcc gtcctcgtac catttaatgg
     3601 acacattacc gggggcaaag gaggtgtttt tcaccttctg gtaaggtttg atccagtcaa
     3661 gaatttttcc ctgttcgccc cagaaggtat caggtacgtt aatagattgt tgatacattg
     3721 cctcgtactg ctgagggttt atcaggcaac ggtctgcgat gttggcagga atggtgtgtt
     3781 tgtgaatttg gctcatgctt ttgttctcct tgtagaatgt taataatatg tgacataagc
     3841 gttaaatgta ggggtattgg cagttttgtt tagtatttgg gcgacagatc acgcaaaagt
     3901 agaattgtgc aaataaacgg cagggtaatt tttgaaggtc agcaacaaaa gttgattaat
     3961 tctttgagga acatgcagtt atgcatgctg ttgaaaaaga ggaagatact gactaactct
     4021 aaagtggtat tttacatgca cttacaattg attaaagaca acattcacag tgtggttatt
     4081 tgttacacat aggggcgagc aatgtcatga cagtgtaggt gcggttactg tcgtgaaaaa
     4141 caataaaaac cgccattgca acaatggcgc aattcggatg aagcccctat gacaaggata
     4201 aaaataaacg cacgccgtat cttcagctta ttgattcctt ttttcttttt cacttctgtt
     4261 cacgctgaac aaacggcagc tcccgcaaaa cctgtaactg tggaagcgaa gaatgaaacc
     4321 tttgccccgc agcatcccga tcaatatctc tcctggaaag ccacctcgga acagtcagag
     4381 cgtgttgacg ccctggcgga agatccacgg ctggtgatcc tgtgggcggg gtatcccttc
     4441 tcgcgcgatt acaacaagcc gcgtggacat gcctttgctg tgaccgatgt gcgtgaaacc
     4501 ctgcgtaccg gtgcgccgaa aaatgctgaa gatggtccgc taccgatggc gtgctggagt
     4561 tgtaaaagcc cggatgtggc gcgtctgatc cagaaagacg gcgaagatgg ctacttccac
     4621 ggtaagtggg cgcgcggcgg cccggaaatc gtcaacaact taggttgtgc cgactgccat
     4681 aacaccgcct caccagagtt cgccaaaggc aaaccggagt taaccctttc ccgtccgtat
     4741 gcggctcgcg cgatggaagc cattggtaaa ccttttgaga aagccggacg tttcgaccag
     4801 caatcgatgg tttgcggtca gtgccatgtg gagtattact tcgacggcaa aaacaaagcg
     4861 gttaaattcc cgtgggatga cggcatgaaa gtcgaaaata tggagcagta ttacgacaaa
     4921 attgccttct ctgactggac taactccctg tcgaaaacgc caatgctgaa agcgcagcac
     4981 ccggaatatg aaacctggac agcgggcatt cacggtaaaa acaacgtgac ctgtatcgac
     5041 tgccatatgc caaaagtgca gaacgccgaa ggcaaactct acaccgacca taaaattggt
     5101 aatccgtttg ataacttcgc ccagacttgt gcgaactgcc atacccagga caaagctgcc
     5161 ttgcaaaaag tggtcgcgga acgtaagcag tcgattaacg acctgaaaat caaggttgaa
     5221 gatcaactgg ttcacgctca cttcgaagcg aaagcagcgc tggatgcagg cgcgacggaa
     5281 gctgaaatga agccaattca ggacgatatc cgtcatgccc agtggcgctg ggatctggcg
     5341 atcgcttccc acggcattca tatgcacgca ccggaagaag gtttacggat gctcggtacg
     5401 gcgatggata aagcggcgga tgcacgcacc aaactggcgc gcctgctggc gaccaaaggc
     5461 atcacccatg aaatccagat cccggatatc tcaaccaaag agaaagccca gcaggccatt
     5521 ggcctgaaca tggaacaaat caaggccgag aagcaggact tcatcaaaac ggtgatcccg
     5581 cagtgggaag aacaggcacg taaaaacggt ctgttaagcc aataaccccg ttccgcctcg
     5641 caaggggcgg aaaacacaat ggagtgaata tgagcgtatt acgttcgtta ttaactgcta
     5701 gggtgctggc gtcgggcctg ttgtggagcc tgaacggaat taccgctacc cctgcggcgc
     5761 aggcatctgg tgatcgctac gaagttaccc agcagcgtaa cccggatgcc gcctgtctgg
     5821 actgtcataa accagatacc gaaggtatgc atggcaaaca tgcttccgtt atcaacccga
     5881 ataacaaatt gccggtcacc tgcaccaact gtcacggcca gccatcaccg caacaccgcg
     5941 aaggggtgaa agatgtgatg cgctttaacg agccgatgta caaggtcgga gagcagaaca
     6001 gcgtctgtat gtcctgtcat ctgccggaac agttgcaaaa agcgttctgg ccgcacgatg
     6061 tccacgtaac caaagtggcg tgcgccagct gccattccct gcatccacag caagatacga
     6121 tgcagacgtt aagtgacaaa ggacggatta agatttgcgt cgattgccac agcgatcagc
     6181 gcaccaatcc gaactttaac ccggcgtctg ttccgttgct taaggagcag ccatgacctg
     6241 gtctcgtcgc cagtttctca ccggcgtcgg cgtgctggca gccgtcagcg gcaccgcagg
     6301 gcgcgtggtg gcgaaaacgt tgaatatcaa tggggtgcgt tacggcatgg tgcatgacga
     6361 gtcgttatgc atcggctgta ccgcctgtat ggatgcttgt cgagaagtga acaaggtgcc
     6421 ggaaggcgtc tcgcgcctga cgattattcg tagcgagccg cagggcgaat ttcctgatgt
     6481 gaaatatcgc ttcttccgta agtcttgcca gcactgcgat catgcgccgt gcgttgacgt
     6541 ctgcccgacc ggtgcgtctt ttcgtgatgc cgctagcggg attgtcgatg ttaatcctga
     6601 tctctgcgtc ggttgtcagt actgcatcgc cgcctgtccg taccgcgtgc gctttatcca
     6661 tccggtcacg aagacggcgg acaaatgcga tttctgccgt aaaaccaatt tgcaggccgg
     6721 taagctgccc gcgtgcgttg aggcttgccc gacgaaggcg ctgacgtttg gcaatctgga
     6781 cgatcctaac agtgagattt cgcaactgct gcgccagaag cctacttacc gctacaagct
     6841 ggcgctggga accaaaccga agctgtaccg cgtaccgttt aaatacgggg aggtgagcca
     6901 atgacgcaga cttccgcatt tcattttgaa tcgctggtgt gggactggcc gattgccatc
     6961 tacctgtttt tgattggtat ttctgccggt ctggtgacgc tggccgtgct gttacgtcgc
     7021 ttctacccgc aggcgggcgg tgcagacagt acgttgctgc gcaccacgct gattgtcggg
     7081 ccgggcgcgg tgatcctcgg tctgttgatc ctcgtcttcc acctgacaag accgtggacc
     7141 ttctggaagc tgatgttcca ctacagtttt acctcggtga tgtcgatggg ggtgatgctg
     7201 tttcagcttt acatggtggt gctggtgctg tggctggcga aaatctttga acatgatttg
     7261 cttgcccagc aacaacgctg gttgccgaag ctggggatcg tgcaaaaggt tctgagcctg
     7321 ctgacgcccg ttcatcgcgg actggaaacg ctgatgctgg tgttggcggt gctgctgggg
     7381 gcttataccg gctttctgct gtcggcgctg aaatcgtatc cgttcctcaa taacccgatc
     7441 ctgccggtgc tgttcctctt ctccggcatc tcgtccggtg cggcggtggc gctgatcgcc
     7501 atggcgatac gccaacgcag taacccgcat tccacggaag cgcactttgt tcaccggatg
     7561 gaaatccccg tggtatgggg tgaaatcttc ctgctggtgg cgttttttgt cggtctggcg
     7621 ctgggcgatg acggtaaagt tcgtgcgctg gtggcggcat taggtggcgg tttctggacg
     7681 tggtggttct ggcttggtgt cgccgggctg gggctgattg tgccaatgtt gctcaaatcg
     7741 tgggtcaatc gcagttccgg cattcctgcc gtgctggcgg cgtgtggggc cagtctggtc
     7801 ggcgtgttga tgctgcgctt tttcattctc tacgccggac agttgacggt ggcgtaagcc
     7861 agaaaagagg tggtttctgg acgtattcct tcctgaagtc ggttttctgg cgttgttgtt
     7921 aagtctcggg gtcaacgtgt tgaccccgtt gacggccttt gcgggagtgc ggttgcgctg
     7981 gcctgccatg atgcgactca cttgcatcgg cattctggcg cagtgcgcgc tcctgctgct
     8041 cgcctttggc gtactgacgt attgttttct catcagcgat ttctcggtca tttatgtcgc
     8101 ccaacatagc tacagcctgc tgtcgtggga actcaaactg gcggcggtgt ggggcggtca
     8161 tgaaggttcg ctgctgcttt gggtgctgct gctttccgcc tggagcgcgc tgtttgcctg
     8221 gcattatcgg cagcaaaccg atccgctatt tccgctgacg ctagccgttt tatctctcat
     8281 gctcgccgca ctgctactgt ttgtggtgct gtggtccgat cccttcgtgc ggatatttcc
     8341 accagcaatc gaaggccgcg atctcaatcc gatgctgcaa catcccggtc ttatctttca
     8401 tccaccgctg ctttaccttg gctatggcgg tttgatggta gcggcgagcg tggcgctggc
     8461 gagtctactg cgcggcgagt ttgatgcggc cagcgcccga atttgctggc gctgggcgtt
     8521 acctggctgg agtgcattaa cggcggggat catcctcggt tcctggtggg cctactgcga
     8581 actcggctgg ggcggctggt ggttctggga tccggtggaa aatgcctctt tattaccctg
     8641 gctttctgcc actgcgctgc tgcacagttt atccctgaca cgccagcggg ggatttttcg
     8701 ccactggtcg ctgttactgg cgatagttac tctgatgctg tcgctgctgg gcaccttaat
     8761 tgtccgttct ggcattctgg tttcggttca tgcgttcgcg ctggataacg tccgcgccgt
     8821 gccgttgttc agcctgtttg cactgattag ccttgcgtct ctggctctgt atggctggcg
     8881 agcgcgggac ggtggcccgg cggtgcgttt ttcggggtta tcgcgggaaa tgttaatcct
     8941 cgctacgctg ttgctgtttt gcgcagtgct actgatcgtg ctggtgggaa cgctttatcc
     9001 gatgatttac ggtctgctgg gctggggacg cctctccgtt ggcgcgccgt attttaaccg
     9061 cgcgacgtta ccgtttggtc tgttgatgct ggtggtgatt gtgctggcga cgtttgtctc
     9121 tggcaaacgc gtgcagcttc cggcgctggt agctcatgcg ggcgtgctgt tatttgccgc
     9181 tgggatcgtg gtttccagcg tcagccgtca ggagatcagc ctgaatttac agccgggtca
     9241 gcaggtgacg ctggcaggat acaccttccg ttttgagcgc ctcgatctgc aagccaaagg
     9301 caattacacc agcgaaaaag cgatagtggc actgtttgac catcagcaac gcattggtga
     9361 actgatgccg gagcggcgtt tttacgaagc acgtcgtcag caaatgatgg aaccgtcaat
     9421 tcgctggaac ggcatccatg actggtatgt ggtcatgggt gaaaaaaccg gagcggatcg
     9481 ttacgctttt cgcttgtatg tacaaagcgg tgtgcgctgg atctgggggg gaggattgtt
     9541 gatgattgcg ggcgcattgt taagcggatg gcgggggagg aagcgcgatg aataaaggac
     9601 ttctcacgct actgctgtta tttacctgtt ttgcgcgcgc tcaggtcgta gacacctggc
     9661 aattcgccaa tccgcaacaa cagcaacagg cgttaaatat tgccagccag ttacgttgtc
     9721 cgcagtgcca gaatcaaaac ttactggaat ccaacgcgcc ggtggcagtc agtatgcgcc
     9781 atcaggttta cagcatggtg gcggagggga agaacgaagt cgaaatcatt ggctggatga
     9841 ccgaacgcta cggagatttt gttcgctata acccaccgtt aacgggtcag acgctggtgt
     9901 tgtgggcgct gccagtggtg ttgttactgc tgatggcact gatcctctgg cgagtgaggg
     9961 cgaagcgatg aaagagccca aaataccggt gaaaatgctg acaacactca cgattttgat
    10021 ggtatttctc tgtgtcggca gttatctgtt aagtccaaaa tggcaggcgg tacatgcgga
    10081 atatcagcgt cagcgcgatc cgctacatca gtttgccagc cagcaaaccc cggaagcgca
    10141 gcttcaggca ttgcaggata aaatccgcgc taatccacaa aacagcgaac aatgggcgtt
    10201 actgggcgag tactatctgt ggcaaaacga ttacagcaat tcgctgctgg cgtaccgtca
    10261 ggcgttgcaa ctacgtggtg agaacgctga actgtatgtg gcgctggcga cggtgcttta
    10321 ttaccaggcc agccagcata tgaccgccca gactcgcgca atgatcgaca aagccctcgc
    10381 gctggactct aacgaaatca ccgctctgat gctgctggct tccgatgcgt ttatgcaggc
    10441 gaattacgcg caagccatcg aactatggca aaaagtgatg gatctcaact caccccggat
    10501 caaccggaca cagctggtcg agtcgattaa tatggcgaaa ttgttgcagc ggagatcgga
    10561 ttaatcatct ggtgatgaag attatgttat tgagctgcat aaaaaataat cgaatgaaca
    10621 tatgccaaaa ataatcacta atcagtatta ttgcagatta aacaaataaa aatctttcca
    10681 taacaaatgg ttattcatta atcctgacat aaactcgcgt tattatgcat taatgcagcg
    10741 aaaagctctg ttgttaaagg gttgcgcaac ataccgcgca aatgatactg atcataagcg
    10801 ttaaaaaaat ctacaaacca acgcaacaca attcatgcct ggcagtatgt cacgttctcg
    10861 cgtttctgaa cggggaacgg cgctccattg aggaagttat tctgatgaaa aatataaaat
    10921 tcagcctggc ctggcagatt ctgtttgcta tggtgctggg cattctcctg ggaagctacc
    10981 tgcactacca tagcgacagc cgcgactggc tggtcgtcaa tttgctctct ccggcgggtg
    11041 atatcttcat ccatctgatt aaaatgattg ttgtgccgat tgtgatctcc acgctggtgg
    11101 tgggtatcgc gggtgtgggt gatgccaaac agctcgggcg tattggcgcg aaaaccatta
    11161 tttacttcga ggtgatcacc accgtcgcca tcattttggg gatcactctg gcgaacgtct
    11221 tccagcccgg tgccggggtg gatatgtcgc agctggcgac cgtcgatatc tcgaaatatc
    11281 agagcactac ggaagcggta caaagcagtt cccacggcat tatgggcacg attttgtcgc
    11341 tggtgccgac gaacattgtg gcgtcgatgg cgaaaggcga aatgctgccg atcatcttct
    11401 tctcggtgct gtttggtctg gggctttcct ccctgcccgc gacgcatcgt gaaccgctgg
    11461 tgaccgtgtt ccgctccatc tctgaaacca tgtttaaagt gactcacatg gtgatgcgtt
    11521 atgcaccggt gggtgtgttt gcgctgattg cggtgacggt ggctaacttt ggtttctctt
    11581 ctctgtggcc actggcgaaa ctggtgctgc tggtgcattt cgccattctg ttcttcgcgc
    11641 tggtagtgct gggaattgtg gcgcgcctgt gcgggttaag cgtctggatc ctgattcgta
    11701 ttctgaaaga tgagctgatt ctggcgtact ccactgccag ctctgaaagc gtactgccgc
    11761 gaattattga gaagatggaa gcctacggag caccggcgtc gatcaccagc ttcgtggtgc
    11821 cgaccggtta ctcttttaac cttgatggtt cgacgctgta tcaaagtatt gccgctattt
    11881 tcatcgctca gctgtatggc attgacctgt ccatctggca ggaaatcatt ctggtgctga
    11941 cgctgatggt gacctcgaag gggattgctg gcgtgccggg cgtgtcgttt gtggtgttgc
    12001 tggcaacgct gggtagcgta ggtattccgc tggaaggtct ggcgtttatt gctggtgttg
    12061 accgtatcct cgacatggcg cgtactgcgc tgaacgtggt gggtaatgcg ctggcggtgc
    12121 tggtgattgc caagtgggaa cacaaatttg accgcaaaaa ggcgctggct tatgagcgtg
    12181 aagtgctggg caaatttgat aaaactgcgg atcaataatt gaagattgcc ggggttatcc
    12241 acccggcaat gtgtggatgc ctgatgcgac gctggtgcgt cttatcaggc ctacgccaga
    12301 cagcgcaata gcctgattta gcgtgatttt gtaggtcgga taaggcgttt acgccgcatc
    12361 cgacatcaac gcctgatgcg acgcttaacg cgtcttatca ggcctacctt aaccgttggt
    12421 taatttttca aactcttcac acccggtatc aaacccttcc atacagctca ggttcaacca
    12481 gtgcagcgcc ttttgtttgt tcttctcgat aaaaccttct tcaccgttta agaacatcat
    12541 tcccgcccag tactcggaat aaccggttcg ggaaattgca gagctgcgtt tgaaatacta
    12601 ggttgccttg tcatcgtcgg ccttaatacc cacgccgttg gcgtaaatca aaccaagcag
    12661 catttgggca tcgacggcag agtcgttctc cagatcttcc gaggcttttc taacagcgaa
    12721 atcgcctttg gataatccgg tttacccgct tgagtattta ccagaatatg cgccagcgtt
    12781 acttcacctt ctttactgcc cgcttgcgcc gctttctctg caagaacttt tgcttgtgga
    12841 tagtccagac tgcccggatt ggtgattttg atctgcgcca acagcgcgca ggcatcagca
    12901 tcaccgctgt cggcggcttt ctgtgcccaa tattcggctt tgctcaaatc gccggagcta
    12961 aaccagctgt cggcaagaaa atattgtgcg cgtcggtccc cggcctctgc tgcctttaaa
    13021 tactggctgc caggctcgga gtcgttggcg tgggcaaaga atgtcaaaaa caacatcaat
    13081 gcgataattt ttttcatttt gataatacga ttgggtaggg agtaaccagt ataaagagtt
    13141 cgaatggata aaaaaacagc ctccgaaagg aggctgtaga aaggactgta ttacgccagt
    13201 gccgcttcgc gcaggcgagt tttcaacttg ttgtactcgt caatcacgta ctgctcggcg
    13261 gcgcgctgat cggcgatcgg ctcgacgcgc acggcgcagt atttgtattc cggcgttttc
    13321 gtaatcgggc ttaagttttc ggtaaccagc tcgttacagg caccaatcca ccactggtag
    13381 gtcatgtaaa tcgccccttt gttcggacga tcgctgacct gcgcacgggt gataattttg
    13441 cctttacgcg agtgcaccca aaccaatgcc tcatcttcaa tacccagacg tttggcgtct
    13501 tcggtattga tttgtgcgta gccaggttca tcagccagcg ccgccagtgc cgcacagtta
    13561 ccggtcatcg aacggcaaga gtagtgacca acttcacgca ccgttgacag taccatcggg
    13621 tactcgtcgg tgagtttgtc gattggcgct acccagtcgc aggtgaagaa ctgcgccaga
    13681 ccgttcgggg tatcaaactt ctctttaaac agataagaag tcccctgatc ggcatctgaa
    13741 gtatcgcggc aaggccactg aatgaagccc tgttcgccca ttttctcgta agtcgcaccg
    13801 tagaaatccg ggcacagatg acgcaactca tcccagatct cctgggtgtt gttgtagtgc
    13861 atcggataac ccatacgggt ggcgatttca ctgatgattt gccagtccgt tttcagatcc
    13921 catttcggtt caactgcttt gaagaaacgc tggaagccac ggtcagccgc agtaaacacg
    13981 ccttcatgct cgccccacga cgttgacggc aaaatgacat ccgccgccga cgcggttttg
    14041 gtcataaaga tgtcctgaac gatgaccagt tccagatctt caaaggcttt acgtactgcc
    14101 gacagctccg catcagtttg cagcggatct tcgcccataa tgtacgcggc acgcacttcg
    14161 ccatgcgctg cgcggtgcgg cagctcgctg atgcgatagc cggtatgagc aggcaggctt
    14221 tccacgcccc aggctttggc gaatttctcg cggttagccg gatctttcac gtactggtat
    14281 cccggatacg tatccggcag cgcgcccata tcgcaggcac cctgcacgtt gttctgacca
    14341 cgaaccgggt ttacacccgc atgcggctta ccgaggttac cggtcagcat cgcgaggctg
    14401 gtcagagaac gcacggtttc cacgccctgg tagaactggg ttacacccat gccccacagg
    14461 atggcggcgc ttttcgcctg ggcatacatc cgtgccgcct gacgaatctc actggcgctg
    14521 acgccggtga tatcttcaac cgactccggc gtgtagcctt caacgatttt acgatactct
    14581 tcaaagcctt ctgtacggga agcgacgaac gctttgtcgt acagattttc ttcaataatg
    14641 acatggccca tcgcattcaa cagcgcgatg ttcgagccgt ttttcagtgc aatgtgcatg
    14701 tcagcaatgc gcgcggtttc aattttgcgc ggatcgcaga cgataatttt cgccccgtta
    14761 cgtttagcgt taattacgtg attcgccatg attgggtggg aatccgccgg gttgtacccg
    14821 aaaacgaaca ctaaatcggt attatcaatt tcgttaatag cattgctcat tgcgccatta
    14881 ccgaccgatt ggtgcagacc tgcaaccgat gggccgtgtc agacacgagc gcagcagtca
    14941 acgttattgg taccaataac ggcgcgcgca aatttttgca ttacatagtt ggtttcgtta
    15001 cccgtaccac gcgaggagcc ggtcgtctgg atggcatccg gaccgtactt ctctttgatg
    15061 gcgctcaggc gctcggcaac gtaattcagt gcctcatccc aggaaacagg ttcgagtttg
    15121 ccgccacgct ggcgacggat catgggggtt ttcaggcgcg gggtcaggat ctgggtatcg
    15181 ttaatgaagt cccagccata ataacccttc agacacaggg taccctggtt ggttttcccc
    15241 tgcgctgcct ccgcccggac gattttgccg ttatcgacga ccaggttgat tttgcaacct
    15301 gatgcgcaat aggggcaaac cgtgacgact tttttcatcg gtctcgctcc agttaatcaa
    15361 atcacgcata cgcgctctcg actacagtat gcatctttta tgccacattt tatttggggt
    15421 cattccctga tattacgggc actatttatt caaaactctg acgaaaaaca ggctgtcgtc
    15481 agttttgacg tgacgaaacg aaataccgcg tgacagccat cacgcggcag acattttatt
    15541 ttttctcgac gacgggagcc gcctgatacc cgccgcccag cgatttcatc aactgaatgc
    15601 tgtggatcac ccggcggctg tccagcatca gtaatgacat ctcttcggca agcactggca
    15661 accgggcttc ggtggcctgt aagcggctgg ttaagccgcg ctgataggcg gcctcggcag
    15721 cgcgctgggt gaagcgcgtt gcttccacgc gttcagcctg catttctcgt tcgtcgttga
    15781 gcgtttgcag acgcgtgccg ttgacggcaa cgtcacgcac cgcgttcagt actgactggt
    15841 tgtaacgttc aatcatcatg ttgctggcgg cgcgagtgcc ttcgagattg gcattcaacc
    15901 gtccaccgtc aaacagcggc aatttcagac ccgggatgaa gttgaactgg cgactggttt
    15961 ttttgaataa ggtatccagg tggatggagt ccagaccgaa aaacgctttg atatcaaagc
    16021 tcgggtagaa cagtgcccgc gcggaatcca cctgatctaa tgacgcctga acataccagc
    16081 gcatggcttg cagatccggg cgtctggcga gcaactcata agagagtgtc gccggaatgc
    16141 cggtctggac tcgtggtaat gccaccggtt tgatctccgg catatcgctg gcccccgcgc
    16201 caatcaatgc acgcagagat tctcgcgttt cggtgatttg ccctttgacg gcagcaattt
    16261 gtttatcgac cgccagaatc tgtgcccgcg cgccgtggaa aggcacttgt gcttccagac
    16321 cgtgcgccac tttactctgg tgcgctttca ccgcgtaatc aatcacatcg cgagtttgtt
    16381 ctaacagatc gagcatctga tagctggcct gcatactgta ataaagctgc gctatgcccg
    16441 tggtcagcga tagctctact gctgcggttt ctgccagcgc ggcattatgc gcgccaatgg
    16501 cggcggcaac cgctgagcga tgcacacccc acaaatcaag atccagtccg gcaaacaaac
    16561 ctactgtggc ttccgtatag tacggcccgt ccatacccag tgagggcgca tccatcgcat
    16621 aagggcttaa aaagccgttc gccgagacgc gttggcggtt gagcatccct aacgctgcga
    16681 cctgtaattg tgaaccggca tctaacaaat cggcctggga ctgcgctttt tcttcccgca
    16741 gtttcgcttc ggcgagggtg tgtgaaccac ttagcgtccg ttggatcagc gcatccagct
    16801 gcgggtcatt gagttgtttc caccactgcg cctgcggcca gccggagctg gcaagatgaa
    16861 tatcgtcggc cagtttgatt tgttccggtt tgagctgttg atgaggtgca gaatccttac
    16921 gtaccagggc acagccggaa atcagcgtcg tgctgccgag aatgctgcac aacagcagac
    16981 gtgaaagttg acgattgatc attgcgtggc tccttgtgcc tgttccgtgg cgggcgttaa
    17041 tgccggggct gtccagtctg gcaagcgggc gaaaagctgg cagacgtgtt gctcctgttt
    17101 taataaggtg ggcaggatgg cctgcgaggc gggtgtctct tcaagcgtta tttgaggaga
    17161 acagctgagt gcggttgcca gaccggcagc gtatttttcc agggcgtcgg caaactgacc
    17221 tgctctgtcc ggctgtagtg cgttatccag cgcctgcgcc gagttccagg tggcatccca
    17281 ggcgtgaaga agatcgcggc cctgacgaat aaccgtttgc gaacgttcaa tcagtaaggc
    17341 gcgttcttcg ctgtccagtt gacgctccag cgccacgcgt tggcacatct cttcacaggc
    17401 attaaacgcc gcatgcagac ctatacgaat ttgcagataa gtgcgcagag ccgtgacttc
    17461 ctgctggcgt gggatccgca ttactttact tagcataccc agcgcaccgg ctagtttttg
    17521 cggcagagtg cgcgcttcac tttcaggcca gacaaaggtg taaatcaccg cggacaccac
    17581 ggtaccaatg atgataccca gggcgcgatc gcgaatttcc accaggtcgt acactgggcc
    17641 aaaaacgttt tcgagcgtgg cgagcgcgaa ggtgacgacc atctgtgtgc cgatataaga
    17701 agagcgttca gagctggtgg caatccatgc gcccaacagg aaaatcggtg ccagcacaaa
    17761 cagcaattcg acaatattgt ccagccaggg catgaccagt agcgtgaata acagcgccag
    17821 aatcgcgccg caaaaggccc cgccaaaacg cagcaccatc ttctggtacg acgaaccgac
    17881 atttggatta gcgacgatca cgcaggtcag catacaggtg tgaatgcctt cccagtccac
    17941 gccgctgtaa aaggtgtaac agatcaaaca ggcgagcaat gtttttaccg cgtagcgtat
    18001 atagtctgga ttggtaaaag catcggcgac cattgatggc ggtttggcgg cgggcgttgg
    18061 cggcgtattc gggttcatct ggcccagttg taacaacgtc tggcagatat tctccaggtt
    18121 acattcccgt gccgccaccg cttcactttc actgatccgc cagtcgcttt gccagcactg
    18181 accttcagca acggcatgct gcagcttgtt gatttctgag gctaattttt gccgaaattc
    18241 aataattgcc tgagaatcag caaaagaggt gggatcgtag cgattcagcg tcgagtaaat
    18301 gtaggttacc gttgccacgc agctttgcca ccatgcgctt tgagttcgcc agttggcatc
    18361 gtccgcgaga caaaagacat tcagtttttg cagcgccagc gcctctcttt caatccgcgt
    18421 ttcgggtagc ggtgcgaggc tgtctgtcag gtgactaatg gcatcatcaa gccgatcatt
    18481 aagcgcctga tgcatttgcg aaatggcacg actgggaaac cacagcacgc cgattaacgt
    18541 catcagcaag gttggataga ggccaacaac gatacaccac agcgttaagc gcacgaccac
    18601 ttccggatag tcgagcatgg cggggaaggt ttgcccgtaa atggcgacaa tggcgacagc
    18661 gaaaaagacc agtcccagcc gatgggtgcg catcaaaaac atgcagccca tcaggatcgg
    18721 cccggcgatg atcaatcgga tcaacggttc gccgtatgac catttgtaga tcaaaaacag
    18781 gctgccgatc tccagcaccg tggcaaccac aaacaagatc gccacaaatt tggtgtaaaa
    18841 cgcgttcgac tgaataccgt aaaacagcac tgccagcgat aacgccacaa aagggatctc
    18901 aaaagtcatc gagatcagaa tcaccagcag gcagcctacc cagagttgca cgatctgcgg
    18961 cacgcggccg ggtcgccgct cgcttaactc ttcatgaaag aacgccagca gcctgaccac
    19021 cggtaatggc agggagttga gcgcgctcat tattgcggct caagattagc gactgccgaa
    19081 gcgccgatgc ggaacatttc cgggtcaggt ttatcgacca tgattttgac cggaaaacgc
    19141 tgggcaacgc ggacccagtt aatagagcga gaaactttcg gcaggccgcc cagcaccagg
    19201 ccgccgtcat tcggtagcac accgtagcca atcgaatcca ctttaccctc gaaggttttg
    19261 ccgctgtcac tcatcaggcg aatcgttgcg ggtgtacctg agcgaatatt tttcagatcg
    19321 gtttcgcgga agttggcgat cacgtaccag tgacgagtgt cgattagggt aaaaataggg
    19381 cgcatggcag aagcaaattg cccgacggag gttttgaggg aaatgacccg gccatcaaac
    19441 ggcgcgcgaa cggtcgccat ttccagatgc agtttggtca gggcaatatc cgcttcgacc
    19501 gccgcacgct gggcaactaa tacatccacg ccgctgacgg cgctggcggc tgactgcgcc
    19561 tgtaacaata cggcattaag atccgcttct gcggcgcgct gcgccgttct tgcacggtca
    19621 acatcttccg ctgagacaaa accttctttc agtaatggct cggtgcggcg taatgtatct
    19681 gtggcctgtt tcgcggcggc acgtgctttt tctaccgtgg cattaaccga gtcggcacca
    19741 aactgttggg cgtcaacgct acgctgggtg agcataattt gcttatccag cgccgcgagg
    19801 gaggcttcag ctttcgccag attggcttcg tacgggcgcg ggtcaatgcg gaacagcaaa
    19861 tcgccctgtt tgactgcctg gttgtcggtg accgccagtt ctacaatgcg gccgctgact
    19921 tccggcacca catcaatggt atctgctgac gcgtaagcgt cattagttga tggcgcactg
    19981 tctacgcgcc agataacgaa aacaagggca accagcgcca acgcaaccac taacagagca
    20041 gggaatttac tgcgaggagc ttttttcggc gtactttcca tgagcgcgtc tcaaatagat
    20101 tagaaaaatg ccagccagaa cagcatggcg tagacagcaa aaagggcggt ataaataatt
    20161 ccgacaaatg ccagattgat atttgcccgc tgaataattc gcctcgtgat tagcgtcaaa
    20221 ataaggctgg caatggcgca gaaaaaccag ctgggataat aagcgccgat caccgggatt
    20281 gccggagata atgagcatcc cgaaaccatg aagacgggaa ttatccaggc tgtttttttg
    20341 agttgcatcg ccatgcgaga gagaactgtt ggcatattaa atacatcctg tttgctgcga
    20401 acaatatctg tagtgaatat caatatccat tgaaggcgtc agtatggcta ttttcttgat
    20461 gaataaaaat agctttattc agccaccgca ttcataactt atatatttta tgtgttaatt
    20521 gattgtatgt atttttataa taaatgcttt tgggggctta agcgccccca aaagtgagtg
    20581 gcattaattt ggggttacga tattcaccca caaatcgaaa ttatccagac aggcaataat
    20641 ttcttccagt ttcgcgccat tgccaataat tttggctttc ttcgctttta ccagatccgc
    20701 cattttggct tgtcctgtca gcacggcgtg cagatcttca cggctgatgt agaaagaggc
    20761 gtcggcttgc ggttgcagtg ttttacggta gttaagcacg ctatcgttca gcgtcaggtt
    20821 gaggttatcg ccgttgctca tattgaagtt caggctgata tttttacccg cagctttcgc
    20881 gctatcgagg cgaacggaca taaagtcgaa cagcatttcg accgacatcc cgcgaatggt
    20941 gtccggggaa ccggtggtgc cgtggctgaa cttatgcacc ccttcgcgca gctctttcgc
    21001 gccggtcagg tagaaaccgc gccaggtggc ggactcggcc tgatagccca gctgttcaaa
    21061 gttattcgct tgcagattct tcgcgacctg gtcacccggg ttggcggcaa tcacctgttt
    21121 cagcagttct gccgaccagc ggtaatcacc ttgcttgttc gcttcttgcg ccaggctgat
    21181 gacacgggca gaaccgccca gcgcctgcac gtaacgttta cccatctcca cctgaccata
    21241 cggatgcagg ttagccgggt taccgtcgta atagccaaga tagaagttat acaccgcgcg
    21301 ggcgttgtgg ctgacagaac cgtaatagcc acggctggcc cagttattgg caagtgcagg
    21361 cggcagttta atcatgtcgc cgatttcatt catggtgtag ccctggttcg ccaggtgcag
    21421 ggtctggtcg tgaatgtact tgatagtatc gcgatattta ccaatataat cattgatatg
    21481 cttattgccc cggaccggcc aggtgtgcgg cataaacagg acttccgcgt cgttacccca
    21541 catatccagc gtttcgttca gatactcggt ccacttgctg gtgtcgcggg ttttcgcgcc
    21601 gcgcagagtg tagaagttgt gcagggtatg cgtggcgttc tcggcggtac acagcgcttt
    21661 cagggccgga atatagaagt gcatttcggc tggtgcttcg ctacctgggg tcatcaggaa
    21721 gtcaaactcc aggccgtcga taatcatctt ctcaccggtt ttggtgatgg ttttgttcgg
    21781 tgcgatgatg ctcgggtagc ccgatgccag cgttacgcca agaccattgc cgatgttacc
    21841 ctgagggtta tgcgccagca gcaggccgta ggagtaaagt gcacggtggc tcataatatt
    21901 acccgccagt acgttttcgc tgatggcttc gtccataaag ccagcagggg cgataacctg
    21961 cactttaccg gatttcacat cagcttcaga gataatgcct ttcacgccgc catagtggtc
    22021 ggtatggctg tgggtataaa taaccgcaac aatcggtttt tgcgggcgat tttggaagta
    22081 aaggtcaagg gcggcttttg cgctcggtgg cgtcaccagc gggtcgatga caataatccc
    22141 ggtatcacct tcgataaagg tgatgttgga gatatcctga ccgcgaacct ggtacattct
    22201 gtcggtgact ttaaacaggc cggaaatacc gtttaactgc
//
LOCUS       DAJTNN010000058        21821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTNN010000058 DAJTNN010000000
VERSION     DAJTNN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.058"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..322
                     /locus_tag="OO150_001912"
     CDS             <1..322
                     /locus_tag="OO150_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5516077.1"
                     /translation="ATAAANKKARQDKLDAEASADKKRDQSYEDELRSLEIQKQKLAL
                     AKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGRDLMKSVGKAEENKSD
                     SWFN"
     gene            complement(417..1994)
                     /gene="guaA"
                     /locus_tag="OO150_001913"
     CDS             complement(417..1994)
                     /gene="guaA"
                     /locus_tag="OO150_001913"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5516078.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2063..3529)
                     /gene="guaB"
                     /locus_tag="OO150_001914"
     CDS             complement(2063..3529)
                     /gene="guaB"
                     /locus_tag="OO150_001914"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5516079.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            3691..5061
                     /gene="xseA"
                     /locus_tag="OO150_001915"
     CDS             3691..5061
                     /gene="xseA"
                     /locus_tag="OO150_001915"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5516080.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5058..5273)
                     /locus_tag="OO150_001916"
     CDS             complement(5058..5273)
                     /locus_tag="OO150_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5516081.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5342..6814)
                     /gene="der"
                     /locus_tag="OO150_001917"
     CDS             complement(5342..6814)
                     /gene="der"
                     /locus_tag="OO150_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5516082.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEVEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(6932..8110)
                     /gene="bamB"
                     /locus_tag="OO150_001918"
     CDS             complement(6932..8110)
                     /gene="bamB"
                     /locus_tag="OO150_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5516083.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8121..8741)
                     /gene="yfgM"
                     /locus_tag="OO150_001919"
     CDS             complement(8121..8741)
                     /gene="yfgM"
                     /locus_tag="OO150_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5516084.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(8759..10033)
                     /gene="hisS"
                     /locus_tag="OO150_001920"
     CDS             complement(8759..10033)
                     /gene="hisS"
                     /locus_tag="OO150_001920"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5516085.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDLNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10144..11262)
                     /gene="ispG"
                     /locus_tag="OO150_001921"
                     /gene_synonym="gcpE"
     CDS             complement(10144..11262)
                     /gene="ispG"
                     /locus_tag="OO150_001921"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5516086.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11289..12302)
                     /gene="rodZ"
                     /locus_tag="OO150_001922"
     CDS             complement(11289..12302)
                     /gene="rodZ"
                     /locus_tag="OO150_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5516087.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLLGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12587..13741)
                     /gene="trmG/rlmN"
                     /locus_tag="OO150_001923"
     CDS             complement(12587..13741)
                     /gene="trmG/rlmN"
                     /locus_tag="OO150_001923"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5516088.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(13891..14322)
                     /gene="ndk"
                     /locus_tag="OO150_001924"
     CDS             complement(13891..14322)
                     /gene="ndk"
                     /locus_tag="OO150_001924"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5516089.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14471..16783)
                     /gene="pbpC"
                     /locus_tag="OO150_001925"
     CDS             complement(14471..16783)
                     /gene="pbpC"
                     /locus_tag="OO150_001925"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5516090.1"
                     /translation="MLRLLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEWPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHYAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(16784..21745)
                     /gene="yfhM"
                     /locus_tag="OO150_001926"
     CDS             complement(16784..21745)
                     /gene="yfhM"
                     /locus_tag="OO150_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR5516091.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPANALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPSDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPLVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGDEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
BASE COUNT         5163 a         6133 c         5408 g         5117 t
ORIGIN      
        1 ggcaacggca gcagcaaata agaaagctcg tcaggataaa ctggacgccg aagcctctgc
       61 ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc agcttagaga ttcagaaaca
      121 aaaactggcg ctggcaaaag aagaagcccg cgttaagcga gaaaacgaat ttatcgatca
      181 ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa tctgaagctg acgccaacag
      241 aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg ggcaaagcag aagagaacaa
      301 atcggacagc tggtttaatt aatcgatgtt agtaacttca agcctatgat tcttgaagat
      361 aaaaaaccct ctgtagtaac agagggtttt gttcattcat agtgcaggga ttaaaatcat
      421 tcccactcaa tggtagctgg cggcttgccg ctgatgtcat acaccacgcg ggaaataccg
      481 ttcacttcat tgataatgcg gttggaaacg cgaccgagga aatcgtacgg cagatgcgcc
      541 cagtgtgcgg tcataaagtc gatggtttcg acagcacgca gagagacaac ccagtcatac
      601 ttacgaccat cgcccattac gccaacggaa cgtaccggca ggaacacagt gaacgcctgg
      661 ctgactttgt tgtacaggtc cgctttacgc agttcttcaa tgaagatggc gtcagcacgg
      721 cgcagcaggt cacagtactc tttcttcact tcacccagca cacgaacgcc aaggcctggt
      781 cccgggaacg ggtgacggta cagcatgtcg tacggcaggc ccagctccag accaatctta
      841 cgcacttcgt ctttgaacag ctctttcagc ggttcaacca ggcccatctt catctctttc
      901 ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg ctttaccggt tgcagaagcc
      961 gcagattcga taacgtcagg gtagatggtg ccctgcgcca gccacttcac gtcttccagt
     1021 ttcagcgctt cttcatcgaa cacttcaacg aaaacgcgcc cgatgatttt acgttttgct
     1081 tccggatcgt tttcgccagc cagcgctgac aggaagcgat cttctgccgg tacgtgaaca
     1141 atgttcagac caaagtgatc gccaaacata tccagaacct gctctgcttc gttgagacgc
     1201 agcaggccgt tgtcgacgaa tacgcaagtc agatttttac cgatagcgcg gtgcagcagc
     1261 attgcggtta cggaggaatc cacaccacca gagaggccga ggatgacttt atcgtcgcct
     1321 acctgctcgc ggatgcgagc tacagcatcg tcgataattt tcgctggcgt ccacagggct
     1381 tcacactggc agatatcacg cacaaaacgc tccagcatgt gcataccctg gcgggtgtga
     1441 gtcacttccg ggtggaactg tacgccatag aagcgttttt cttcgttagc cataatggca
     1501 aacgggcagc tttcggtgct ggctacggtg acgaagtcgg acgggatagc ggtaacttta
     1561 tcgccgtggc tcatccagac atcgagcagc ggtttaccgt ctgcggtcag cgcatcttcg
     1621 ataccgcgaa ccagtgcgct gtcgtttacg acttcaacct gcgcgtagcc aaattcacgt
     1681 tcgttagagg cttcaacgtg accgcccaac tgcattgcca tggtctgcat gccatagcaa
     1741 acgccgaata ccggtacgcc tgcttcaaag acatactgcg gcgcacgcgg actgttttct
     1801 tcagtagtac tttccgggcc gccggaaaga ataatgccgc ttggattgaa gtcacgaatt
     1861 tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa cacccagctc acgcacgcgg
     1921 cgcgcaacca gttgagtgta ctgagaaccg aagtccagaa tgaggatgcg atgcttatga
     1981 atgttttccg tcattgacgc ttattccgag gcaagtgaaa cagataatat aaatcgcccg
     2041 acatgaagtc gggcgaagag aatcaggagc ccagacggta gttcggggac tctttagtaa
     2101 tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc gctgatacgt acaaactccg
     2161 ctttagtacg cagttcgtcg atagtaccac agccggtcag gcccatacag gagcgcaggc
     2221 cgcccatctg ctggtgaatg atctctttca ggcgaccttt ataggctacg cgaccttcga
     2281 taccttccgg caccagtttg tcggcagcgt tgtcactctg gaagtaacgg tcagaagaac
     2341 ctttggacat cgcacccaga gagcccatac cacggtaaga tttgtaagaa cggccctggt
     2401 agagttcaat ttcacccgga gattcttcag tacccgccag catggaaccc accattaccg
     2461 cgcttgcgcc agcggcgata gctttggcga tgtcgccgga gaagcgaata ccaccatctg
     2521 cgataaccgg aatacctgtg ccttccagcg cttcaactgc gtcagcaacg gcagtgatct
     2581 gcggaacacc tacgccagta acgatacgag tcgtacagat agaaccaggg ccgataccga
     2641 ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc acctgcagct gttgccacgt
     2701 tgccgccgat gatttgcaga tccggatatt tagcacgggt ttcacggata cgctgcaaaa
     2761 cgccttctga gtggccgtgg gaggagtcga tcagcagaac gtcaacgcca gcggcaacca
     2821 gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac tgcagcacca acacgcagac
     2881 ggccttgctc gtctttacag gcgttcggtt tacgttccgc tttctggaag tctttcacgg
     2941 tgatcatgcc gatcaggtgg aattcgtcat caaccaccag cgctttttca acgcgttttt
     3001 cgtgcatttt tgccagcacc acttcacggg cttcaccttc acgcacggtg accagacgct
     3061 ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc ggtaacaaaa cgcacgtcac
     3121 gaccggtgat aatacccacc agttcgtttt cttcggtaac aaccggatag cccgcaaaac
     3181 cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt ggttggcaga acagtctgcg
     3241 gatcagtcac cacaccagat tcgtgttttt tcacacggcg aacttcttct gcctggcgtt
     3301 caatggacat gtttttgtgg ataaagccga taccgccttc ctgagccaga gcaatagcca
     3361 ggcgcgcttc cgttacggta tccattgctg cggaaagcat agggatattc agacgaatag
     3421 ttttcgtcag ctgggtgctg aggtcagcag tattcggcag aacggtagaa tgagcaggaa
     3481 cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat acgtagcatg ggcaatatct
     3541 cgaccagagt ggttaataaa tattgccgcg gcattataca gagcgtaacc gattgcatct
     3601 accccttttt gcaaaaaatg cttgctatcc ccgaagggcg ggttactatc gactgaataa
     3661 cctgctgatt tagaatttga tctcgctcac atgttacctt ctcaatcccc tgcaattttt
     3721 accgttagtc gcctgaatca aacggttcgt ctgctgcttg agcatgagat gggacaggtt
     3781 tggatcagcg gcgaaatctc taatttcaca caaccggctt ccggtcactg gtactttaca
     3841 ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc gcaacagcaa ccgccgggtg
     3901 accttccgtc cacagcatgg acaacaagtt ttagttcgcg ccaatattac gctctacgag
     3961 ccgcgcggtg actaccagat aatcgttgag agtatgcagc cggccggtga agggctgctg
     4021 caacagaagt acgaacagct caaagcgaag ttgcaggctg aaggtttgtt cgatcagcaa
     4081 tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg tgatcacctc aaaaaccggt
     4141 gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg atccttctct accggtgatc
     4201 atctacccca ccgccgttca gggcgatgac gcaccggggc aaattgttcg cgccattgaa
     4261 ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg ggcgcggcgg cggttcgctg
     4321 gaagatttat ggagttttaa cgacgaacgc gtagcgcggg cgatttttgc cagccgcatt
     4381 ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca ttgccgattt tgttgccgat
     4441 ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga gccgtaatca gcaagagtta
     4501 ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg cgatggatta ttatctcgcc
     4561 aaccgcacgc gtcgctttac gcagatccat caccgattac agcaacagca tccgcagctc
     4621 cggctggcac gccagcaaac catgcttgag cgcctgcaaa agcggatgag ctttgcgctg
     4681 gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt taacacagcg gctgaatcag
     4741 caaaatccac agccgaagat tcatcgcgcg caaacgcgca ttcagcaact ggaatatcgt
     4801 ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg aacgtttcgg taatgcagta
     4861 acgcacctcg aagccgtaag cccactgtca acgctggcgc gtggatacag cgttactact
     4921 gctactgacg gcaatgtact gaaaaaagtg aagcaagtta aagcgggtga aatgctaacc
     4981 acacgtctgg aagacggctg gatagaaagt gaagttaaaa acatccagcc agtaaaaaaa
     5041 tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc gttttttcga tatcaagcca
     5101 tgcccgtgct gacagaagta atctactgca ccgcaggctt ttaacacctg caacggttga
     5161 tggcagtctg gacaaagagc tttcatttca ataatttccc cgcaggatgg gcaacgcgca
     5221 tggccgttat cctgatcaag cacatgctga cattgtggac aatgcagttc catatgaatt
     5281 cctccggtag ccatccctct acattcatag agggaatggc agataaaata cttacggata
     5341 attatttatt tttcttgatg tgcttcatca gacgcttacg tttacgcatc tgggttggcg
     5401 tcagggtgtt acgcttgttc gcatacgggt tttccccttc tttgaactga atacgaatcg
     5461 gcgatcccat tacgtccagc gatttgcgga agtagttcat caagtagcgc ttgtaggaat
     5521 caggcaggtc tttcacctga ttaccgtgaa tcaccacaat cggcgggtta taaccaccag
     5581 cgtgggcata tttcagcttc acacgacgac cgcgtaccag cggcggttgg tgatcttcaa
     5641 cagccatcgt catgatgcgc gtcagcatag aggtccccac acgacgggtg gagctgtcat
     5701 acgcttcacg tactgattca aacaagttac caacaccact gccgtgcaag gcagagataa
     5761 agtgcacacg agcaaaatcg ataaagccca gacggaagtc cagcgtttct ttcacctgct
     5821 ctttcacttc ctggctcagg ccatcccact tattcaccac aatgacaagt gagcgcccac
     5881 tattgagaat aaagcccagc agcgagagat cctgatcgga aataccttcg cgcgcatcaa
     5941 tcactaacat caccacgttg gcatcttcaa tggcctgcaa cgttttgatt acggagaatt
     6001 tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc agcggtgtca atgagcacat
     6061 actcacgtcc atcgcgttcc attggaatgt agatactgtc acgcgtcgtg ccaggcatgt
     6121 cataaacaac aacgcgctct tcaccaagaa tacggttagt gagtgtagac ttacctacgt
     6181 tcggacgacc cacaatcgcc agtttgatcg gcagactttg cgggtcgaag tcgtcttcct
     6241 cttcttcttc gccgttctct tccacttcaa attgcgccca gtattcagcg tcttcgtcga
     6301 cttcctcttg cggtgcgaga tcttccatcc acggcagcag cacatgctcc agcagactta
     6361 atacgccacg accgtgagac gcggcgatcg ggtagatttc acctaagcca agcgagtaga
     6421 aatcaaccac tgcctgatcg ggatccagac cgtcagtttt gtttgccacc aggaaggtcg
     6481 gtttttcacg ggagcgcaga tgtttggcaa tcgcttcatc tgccggcatc aggcccgcgc
     6541 gcgcatccac cataaacagt acgacgtccg cttcttcaat cgccagcagc gactgttccg
     6601 ccatgcgggt ttctacaccg tcttctgtgc catcaatccc gccggtatca atacagataa
     6661 actcacggcc ttcaatttcc gcacgaccgt acttacggtc acgagtcaga cccgggaaat
     6721 ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa taacgtggat tttcctacgt
     6781 tagggcgccc gacaagcgcg accacaggta ccatgtttaa agcctcattt ttataaatca
     6841 tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca ggaaaacggc ccctgtccag
     6901 gagccgtttt caaagtgaac gacagagacg attaacgtgt aatagagtac acggttccgt
     6961 cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg ttcagtctgg aaaccggaac
     7021 tatcaacttt ttgctgggca acgaaacgac catcttcgac attaatccag tgcagataac
     7081 cttcactgtc accgaccacc aggttgccat catacagcac cggagaagtc agcaggcgat
     7141 gcaacagatc gctttgtgtc cacagcgtaa cgccgccatc aatggtcaac gccatcaccc
     7201 ggtcattttg atcgaccaga tagatgcgat tgccgtcgac gatgaaatca ttcaccgaac
     7261 ccagttcgcg tttccacata atctgaccac tgcgcagatc aagcgccgtc aggttaccat
     7321 tataggccag cgcgaaaaca acgccgttaa caacgacggg agtcgtgtca acatcgctca
     7381 gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg ctgctgccaa atcatctggc
     7441 cctgttccat cagcactgcg ctgacgcgac cattatcgcc ccccacgacg gccgcaccaa
     7501 aagccgttgc cggcgcagac tcgccacgca aagagagcga aggcatatcg aggttaactg
     7561 tccatttgac agcgccgtca gcttcgttca gcgcttgtaa ctgaccgtta ctggtgtgga
     7621 ttaacaccag accgtcgctg accaccgggc gcgaaagtgc ttcacccgcg actttagttt
     7681 gccatgccac agtaccatcg ctggtattca gcgcgtaaac ctgcgccttt tcgctgccaa
     7741 tgtagacatg cccaccagac acggtcacac cgccagaaag taatgcaggc tctttagaga
     7801 accagccatc tttctcggcc aggctgacag accagatttc tttgccatca tccgcattca
     7861 gcgcttttac caaaccagcg cggtccgctg catagacaac gttgtccgcc agtgccggat
     7921 gaagattgga atagaagttg ccaatgccgc taccaacgga agtactccac gccgtggtcg
     7981 gcgtaaactg gttttcaacg gttggcaatg gggacatctt taccacatct tcttcgctgt
     8041 taaacagcga acagccgctt aaaagggtaa cggaaagcag tcctggcagc agtaatttac
     8101 gcaattgcat cgggtccctc tcagatggac aaattattaa ttttcatctg catcatttcg
     8161 ctcagtgccg gagtaacatc gcttttcacg cctgcttccc atgcactacg cgcaccttgc
     8221 ttatcacctt tgctcagcaa tgcttcacca cgcaggtcgg caacaatggc agcccaccct
     8281 tcacctttga tggtatcaag ggttttcagc gcggcatcag cctgcttgag ctgtacctga
     8341 acgcgagcaa gacgcagatt tatcacggct ttgagatttt catcgctcgt gtctgccagc
     8401 ccctgttgta actgggcggc agctttctct agttcatttt tgtcaacaaa ttgctgcgca
     8461 agttccaaag aagccagcgc accataagta tttttatttt cagcagcaaa tttttccgcc
     8521 gccgggatgc tatccggttt gccttcgctc actgcggtaa ccgcattttg ataggcaaga
     8581 gaagcggagc gtgcagaatc aacctgatgg ctgttccagt agcgccagcc aatcagtgcg
     8641 ccaacgccca aaatcacccc aacagccagt gctttgccat tttcagcaaa aaagcgttta
     8701 accgcttcta cctggtcgtt ttcgttctcg taaatttcca cgctgtcctt ctccttcctt
     8761 aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg cgcaactgcc gtttgctcac
     8821 cagagcgcaa atccttcact actgctgtgc cgttagtcac ttcagactca cccagcacca
     8881 cagcaacgcg ggcaccccat ttatcagcac gggcaaactg tttcttaaag ttgccgccgc
     8941 cgtggttggt catcaatttc acgccctgta attcatcacg cagacgctca gctaatgcca
     9001 tagccgcaga ttgtgtatca gcacctgaag ccaccaggta tatatcgaca ataggatcgg
     9061 ctttaaattc cggattaacg gcctgtacta acaatacaag acgttcgagg cccatcgcaa
     9121 aaccgacagc cggtgttgca cgaccgccca gttgttccac aagaccgtca taacgaccgc
     9181 ctgcacacac ggtgccctgg gagccgagac tgttagtcac ccactcgaaa acggtacggt
     9241 tatagtaatc cagaccacgc accagacgct ggtttacggt gtaagcgatc cccgcgctct
     9301 caagcagttt gcacagaccg gcaaaatgct cacgagattc ctcgtccaga taatcaccta
     9361 atgccggagc gtcgttgaga agcgcctgca cttccggatt ttttgaatcc agcacgcgca
     9421 gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag cttttcttta tgctgctcaa
     9481 ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc cagcgaaccg atagaattca
     9541 gctcaagagt tacgtgttcg gaaataccca gcgcgcgcca ccagcgggcg gtgagcataa
     9601 tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac ttcgcagccc aactgatgga
     9661 actgacgata acgccctttc tgcggacgct cgtgacggaa catcggcccg atataccaca
     9721 gacgctgttc ctgattgtac agaagaccat gctcgatgcc ggcgcgtaca cagcccgccg
     9781 tcccttcagg gcgcagagtc aggctgtcgc cattgagatc ctcaaaggtg tacatctctt
     9841 tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa tagcggggtc tgctctacaa
     9901 tcggcaagcg gatttcactg taaccgtagc tgccgagcac gtttttcagt gtgccttcaa
     9961 tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt catgccgcga atggcttgaa
    10021 tgttttttgc cacgtttatt ctctttctga atataaaaat gaaccctcaa cgcttccctc
    10081 aatgtttggg gagccatgcg ggttcaatca tacacgggaa gcgaggcgct tcccatcacg
    10141 ttattatttt tcaacctgct gaacgtcaat tcgacgcgct tcatccagct gactggcttt
    10201 cgcacgaatg cgtgcttcca gctggtcgat catatcgttg ttgtccagac ggtctttgcg
    10261 cacgccatct tcatagaggc cgcttttctt gttgccgccg gtgacgccga gtgtagaaac
    10321 cagcgcctca cctgggccat tcaccacgca gccgataata gaaacgtcca tcggagtgat
    10381 gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg ataacatcaa attcctgacg
    10441 cgaacaggtc gggcaggcga tgaagttgat ccctcgcgaa cggatacgca gcgatttcaa
    10501 aatatcgaaa ccgactttga tctcttcgac cggatcggcc gccagcgata cgcgcagcgt
    10561 gtcgccgatg ccttcagaca gcagcagacc taaaccaatg gcggatttta ctgccccgct
    10621 gcgtgcacca ccggcttcgg tgatccccag atgcagcggc tgatcgatct gttttgccag
    10681 caaacgataa gactcaacag cgaggaagac gtcagacgct ttcacgctga ctttgaactg
    10741 atcgaagttc aggcgatcga gatgatcaac atgacgcatg gcggattcca gcaacgcctg
    10801 cggcgtcggt tcgccatact tttcttgcag atctttttcc agcgatccgg cgttaacgcc
    10861 aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc accatgcgaa tacgctcttc
    10921 attaccgata ttgccagggt taatacgcag acaatcgacg ccgtattccg ctactttcag
    10981 cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc acgttaaccc gctgtttgat
    11041 gagtttgaac gcttctgccg cgtccatcgt cggtacggag acacggacga tatcagcgcc
    11101 aacgcgttcc agcgccttga tttgattgac cgttgcttcg acatctgtcg tacgcgtatt
    11161 ggtcatggac tgtacggcga tgggagcacc atcgccaatc ggcacattcc caacgtaaat
    11221 acgtgttgat tttctacgtt gaattggagc ctggttatgc atgaaaaatc tcccgcgtta
    11281 cccgtctgtt actgcgccgg tgattgttcg gcattgaggg tcagacgcgc aacctggtta
    11341 gttctgataa aacgactcag atcgacaggt ttcccttgat actggatctg tactgcggct
    11401 ggcgcaccaa ttttcagttt gtacggtgcc tggccggtta agttcaaatt accgtcttta
    11461 cgctgcatac cgctaaacaa ttttttaccg gtagcatcag tgacctccag ccagcaatcg
    11521 gcagtaaagt tcatcaccag cgcattcgga tcagccaccg gcgtggtcac gccagcctga
    11581 tcggttggca acggcgcagc accatctggc gttgttgctg ccgttggtgc cggggtcgcg
    11641 gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat tctgttgcgg atcaacagct
    11701 ggtgctggcg cagttacggc aggtgtttgc gtgttggttg cggtagtatc cacagacgct
    11761 ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg tatttaacgg aacactctgc
    11821 ccctgctcgc tattactgct cagttccgcc gaagattgat cggccatagt ggtgatctct
    11881 tcctgctgag ctttgtggtc ttgccaccac caggcaccgc tcaggccgat aaccacaaac
    11941 aacaccagcc aggtgaaggt catcagccag ccgtcacgtt ttttgcggcg tttaccgagg
    12001 gaaaaactct gcatcggcgc aactttcgca gcccgtagtg gagcctgctt ttccagccct
    12061 agcagcagtt cttcttctgg aatatgtacc agacgcgcat aagagcggat atatccgcgc
    12121 aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt caatgtcgcg taccgtggaa
    12181 accttcaggc aaagtcgctc ggcaacggcc tgctgactaa gtcctagttg ttcgcgagca
    12241 ttacgcaggc gagcgccggt agtaagtgct tcattttggt cgtgcgtggc ttcagtattc
    12301 attcgctgca ggtacgttta aatgagaatt gagatgccgg tgaaccatca tgctcaccca
    12361 caccgcgaaa catccgttaa gttaaccgtt atcatacagt ataagaccgt ctgtgtgctc
    12421 gtgacaaagc ccacattcac taaggctaac ttactgttgc atcgttacat actgccttaa
    12481 agtcagcaaa aacgcaccgt taatattgac cagacaattg caacttaata cttcattcat
    12541 aaaagattac gccacggtac ataaagtaac cgtggcgtaa tggctatcag accgctttaa
    12601 tgtcgatggc ttcaccctgc atccgtttac gcagggtacg tttcgtacgg tcgataacat
    12661 cgcccgccaa ctgaccacag gcagcatcga tatcatcacc acgagtttta cgcacaatag
    12721 tggtgaaacc gtagctcatc agcacttttg agaaacggtc gatacggctg ttcgaactgc
    12781 gtccatacgg cgcgcccggg aacgggttcc acgggatcag gttgatctta cacggcgtat
    12841 ctttcagcag ctccgccagt tggtgcgcgt gttcagtgcc gtcgttaacg tggtcaagca
    12901 tcacgtattc aatagtgact cggccctgat tggcgttgga tttctccaga taacggcgca
    12961 ccgcagcaag gaacgtttcg atattgtact ttttgttgat cggcacaatt tcgtcacgaa
    13021 tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac gtcgatcata tcgcccagtt
    13081 tatccagcgc cggaactaca ccggaagtag aaagcgtgac gcgacgttta gacaggccaa
    13141 aaccgaaatc atccagcatg atttccatcg ccggaacgac gttgttcagg ttgagcagcg
    13201 gctcgcccat gcccatcatc actacgttag tgattgggcg ctgaccggtg acttttgctg
    13261 cgccgacgat tttcgccgca cgccacacct ggccgataat ttccgacacc cgcaggttgc
    13321 gattaaagcc ctgctgggcg gtggaacaga atttacactc cagcgcacac cccacctgcg
    13381 aagagacgca gagcgtggca cggtcgtctt ccgggatata caccgtttcg acgcgctgat
    13441 cgccaacggc gatcgcccat ttaatggtgc cgtcggatga acgctgttct tcaaccactt
    13501 ccggtgcgcg gatttccgcc acctctttca gtttgccgcg caacactttg ttgatgtcgg
    13561 tcatctcatc aaagttgtcg cagcaatagt gatacatcca cttcatcacc tgatcggcgc
    13621 ggaagggttt ttcacctaaa tctttaaaaa actcccgcat ctgctgacgg ttgagatcca
    13681 gcaggttgat ttttccatct ttcgtggtga cgttttcagg tgtgactaat tgttcagaca
    13741 tatgctattc cggcctcgtt attacacgtt atggcccctg gagggttgaa aaaagaaacg
    13801 ccccggtgag cggctgctcg tccgggggcg ctgcattgta caaattctgg cgcacggatg
    13861 ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg cgcgggcaca cttcgccttc
    13921 gccaaagaaa taagcgattt cgcgagcggc agattcgacg gaatcagaac cgtgggtacc
    13981 gttttcggtc aggctgtcag cgtaatcagc gcgcagagta ccagccagtg cgtttgccgg
    14041 attggtcgcg cccagcagat cgcggtgacg ctgaacggcg ttttcacctt ccagcacgga
    14101 aaccacgatc gggccagagg tcatgaattc aaccagacca tcaaagaacg gttttccatc
    14161 gtgttcagca taaaagccac gtgcctgttc aacggtcagg tgcagcattt tggtgccgac
    14221 aattttgaac cctgcagctt caaagcgcgc aaagatatta ccaatgacgt tttttgctac
    14281 cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc atttttacct ctgtaaattg
    14341 ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat tataatgagc aacagggccg
    14401 ttgactattg atgaaggtaa cattttttta aaataaaatg aggtttagca acaatcaggg
    14461 acaaaacaga ctattgcatg acaaatttca ctgtcgcgat ttgccccacg tcatccatca
    14521 ccagcaattg ataatcacct ttatccgtca aatgcagggt cacgttgcgc ccgcgttcag
    14581 ttaacggttc gccattcaga aaccaccagc gttcacctgc ccctccactt gattgcaacg
    14641 gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat cgcgccatcg cgaacgcctg
    14701 tcagctgcag cgggagctgg gcatagtgac cgtatggcgg gcaacttgtc gaggccggtg
    14761 gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg ttccagcggt agcggccaga
    14821 catttatcat ttcttgtcgc gcttgcgggc aatcagcggc aacacgtttg ccattttcat
    14881 ccagccagat ggggaaacga atgccattaa tgccttcctg ctccggcagt aatagagttg
    14941 gcggctgact cccgtccagc agccaggttg ccaggcggcg gcgacagtta ccgtcacctt
    15001 ccggcaaaga ctgtccgccc ggccagcaga taacgccacg agtgactgag ttcgggcgcg
    15061 ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt attgacctga ttcaacaatg
    15121 gtacggcact ggcaaagcca aactgaccaa caacgggcgt gccgtccggt ctgccagtcc
    15181 agatcccaat gacatagcga gcgttaaccc caatcgccca ggcgtcacga tagccatagc
    15241 tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa ggcactatcc ggcaagggtt
    15301 gcgcttcatc agccataatc cggcgaatga tccacgccgc ccccgacgac attaaaggcc
    15361 attcaagcag cggatcgtca ggctgtaagc gcaatttgcc tgccttgccg tggcgagcaa
    15421 acgcggtata cgctgccgcc atatcttcca gttttgcacc agcgccgccg agaatgagtg
    15481 aaagattcgg cgcagcaccg ttgggcaaat ataacggcaa tccaacattg cgtaactttg
    15541 ccgcaaaccg tttcggtcca taggcttcca gcacctgcac agcaggtaag ttcagcgagc
    15601 gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc gctatcaaag ttacctggtc
    15661 gataatcacc ggtgcgccgg gggacgtctt gcagcagtga tgccgggtgg atcaagcctt
    15721 catccagcgc cagaccataa acaaacggtt tgagcaccga tcctggcgat cggatcgcat
    15781 tgaccatatc aacatgacca aagcgtgaat catcgttgag atcaaccgat cccacccagc
    15841 cgcgaacacg catatcggta tgatcaacca cgatcatcgc cagtgagctg cgcggtggca
    15901 accgcccttt ccagttttgc gccagttctt ccagacgtcg ttgaagaccg gcatccagcg
    15961 tagtaacgat tttgtcgctt ttgcttttac cgagcatcat gcgcgaaaac agcggtgcca
    16021 gttgcggcat ttgtcggggt gccagccaga tgggttcttc ccttgactct tttacctgct
    16081 cacgggacca cacaccttgc acggccatcc gttcgagcac tttattacgc gcggcttcgg
    16141 cacgctccgg ccaacgatcc gggcgcagtc ggctgggcgc ttgcggtaac accgccagca
    16201 ttgccgcctc ggaatagctt aaattcgcag gcgattttcc gagataagcc caacttgccg
    16261 caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt aagatacaag atcagaattt
    16321 cacgcttaga caaatgccac tccagttgca acgcgcgcca gagctggaga attttgccac
    16381 caaatgtttt ggggtgagga tcaagcagac gagcaacctg catagtgagc gtgctgccac
    16441 cagaaataac ccgtcccgaa gtgagatctt gccatgctgc gcgcgccacc gagaatggat
    16501 tcacccccgg atgcttccag aaccagcgat cttcataatt gatcagcgct tcaaggtaac
    16561 gtggagacac atcttcgatt gttaccggat aacgccagat gccatcagca tcggcgaagc
    16621 gccagagcgg cgtaccatcc tgcgccacga ccactcgtgc gggattgact tcatgcagcg
    16681 gtagaggcca gagtttatct gctccccacg ctgcaagaaa aaggagaaat ggcacagctg
    16741 ccagcgttat ccagcagccg cgtttggtta acaagcgaag catttacggt cggacaatca
    16801 gcagatcttc agccgcgccg gtcgcccgcc attggggaac atacattgat tccaccatcg
    16861 gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc cagatacacc agcgttaccg
    16921 gttggtattc atcaacggca accgccgcca caaagcgatc gtcacggaac tcaatgtgct
    16981 taatgctcgc ctgctgcatc tggttcagta agttttgcac ttcgtcacca ctttgctcca
    17041 ggctggcgct accgttcgcc agattctgat tttccagttc cagacccgca ggcagcagat
    17101 ccacgactaa cgcatccggc acgctgttac tggcttttac ctgcaaccag accagcacca
    17161 gatcgccgct acgtaacgag tccagcgatt tgctcttacc atcagtacca agaatatgac
    17221 gctcgatttg cagcacattg ttcgccggta aaggtgcgga ttgcggataa ccgctggcat
    17281 ccatacgcaa ccataacggc tgatcgccac tgtttctcac ctgcaaggtg gcaagttgat
    17341 cgctattcag attgctgttt agcgttttct cacctgtcag cggctcagct gagaaagagg
    17401 tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg ggcagccagg aacaacgcgt
    17461 tactttcctg cgtcgatagc cagcgttcac caaacgcctg ctgcgaaagt gtgttcagca
    17521 aagtgtactg ctcatcgggt agcagtttat tttcttccag caaggagagc attaacgcgt
    17581 tgtcgcgcag tggactaccg taatcaccca gccatatccg ctcatcacta tttctcggcg
    17641 ttttcagcgc cagcacaatc gcttcttcac cacgcgttgc atcacccatg gttttcagcg
    17701 caatgccaag ttgcagcagc ggtaaaccag aagcggcatc tgcacgatgc tcccagattt
    17761 cacgcagcgc acccagtggt gctttttgct gacgggccag caccagcgca gcgtaagact
    17821 gaacggcgaa tttactggct ttgagattat ccgcgtacgg gatcgacatc atgcccggat
    17881 cttgtaaata gcgcagcaga cgctcattac cccggttaat ggcgtctgtc ggcacgctgt
    17941 aaccctgttc gcctgcgcgg accaggaaat ccatcacgta agccgtcagc cagtactctt
    18001 cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc acgttgcatt tgcagcaaac
    18061 gggaaatgcc gatatcgacc gatgcacggc gtttctcatc actgtcgcct ttgatgccca
    18121 acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa caggccgctg gcggtttgct
    18181 caagacagcc gtacggatac gcttttaact ctttgatata acgtgcaatg ttcagcggtg
    18241 gtttgccgct caacaacaat tgcccttcca gcgtaacagg cgagaagttt tgcaatccat
    18301 ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt accgtaatta accgtttgtg
    18361 ccgggaacgc cggacggacg ccgattttcc actgcttatg ctgatcggca acggtttcac
    18421 ccggtaacgc taacccgcta atggtggcct gaatttctcc atcgccataa cccggcaatg
    18481 ctcgcaccgg gataaacagc gtagtacgca cacctggcgc taattcaacg gctgcgggtg
    18541 aatcgctgac cagttcaagc aaaccactgg cggtcagggc aacgttcagt ttttgcggtt
    18601 tatcggtaag attagtgata tccagcgtca gacgcgaggt atcgccactc gccataaagc
    18661 gcggcatgtt cagttcagca atcaccggtg cggcaactat cactttgctt tcgttgctac
    18721 cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa ttcaccgtta aaatcgccaa
    18781 tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt taccggcagc gcctgctgcg
    18841 cgacaatatt gacgtgattg accagcggtt taccaccacg tttcagctca tcaccatcgc
    18901 caccgaaacg cagagctgcc agacgcccct gaccttcaat aacctgaccg taaatatcgt
    18961 aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg ccacggatct ggcgtgacgt
    19021 agtcagtaat attcagcaca ccactatcaa cggcagacac cagcacattc acctgtttag
    19081 gcttctcgcc atttttagtg ctggctttaa ttttcacggt taatggttga ttgggacgca
    19141 tttttgctgg tgtttccagc gccagatcga ggcgacggtt ttcatcgcca agcggcagat
    19201 gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga tttatcgcta ggacgtacca
    19261 ccagcgtact taaatacaga tcatgacgat tccaggtttt atcgaccgga atcgtcagat
    19321 ccagcccttg agccggaaca tcaatctctt gccaccacag cggcccttca ctggactcga
    19381 ccatcgcata acctttaccc gccgttggcg cggcgatatg caacttaatg gtgtcgccag
    19441 ggcgataact ggctttatcc agtttcagcg tgacacggtc gggtcgcact gcgccgctac
    19501 cgtcgctgtt gtcctgccaa ctatagccag cccagaaacg aacactactg accgcttcat
    19561 tcggcgcttt gacttccaga cgataagcgc cccactctac cggaaaactg accttgccgg
    19621 tttcgtccgc tttcagatcc agagtttgtt cattttcgat cagatctttt tgatcaaact
    19681 gtgactgcca gccttcatct tctgaccagt tccagtagta atcgcggcgt tcgcgaatca
    19741 ggcgcacctg caagcccgac acggcttttt tcacgccttg tgcatcgcta taaacgatgt
    19801 caaaagcggc gttactgcct tcatcaacaa tgggctgttt caccgtgctg tcagtgcgat
    19861 aatcgtaaac cgatttcaag gcgaactgcg gacggagccc cggcaatgca ttggcaggcc
    19921 agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc cgattccagc aaactgccct
    19981 ggaaaataac ctgcaatggg gaatgcgttt ccttccactg gctttctgta gaaacttcgc
    20041 cgcgcccttt atcatccagc gtcaactgaa cttcatccag cgtgcgggaa aggttttcgg
    20101 cagctatatc gccgaattca aaaccaggta aggctgacac agcctcacgc agtgggcgca
    20161 ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc accgtacagg tagtatccca
    20221 ccacggagaa tttcacttca tcattcggcg taagcggggt tttctcaccg gtcagattca
    20281 gcgccatacg ctctggcata aaatcttcga cgtggaaatc ccacatccgg tactggttat
    20341 cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc attgctatcg agtggccagg
    20401 taaagtggta gaggccattc tccggctgac taacgacgct cctgagtacc tgcccgtcag
    20461 gtttaatcac gtctaacttg atgggttgat cgggcaatgc tttaccgtct gcatcgcgca
    20521 gtaaaccatt gaggattacc gtttcacctg ggcgataaag atcgcgtggg ccaaacatga
    20581 aaaactgttt gctatagcct ggcgcgccag caatgttaaa ttctgctaag tccagcgccg
    20641 gaagttttaa atcgagtagc gttgtctgac cgtctttacg tgccagcagt aatgccgcat
    20701 ttttatcatt ttccagctgc acatgcccct gagcgtcact ggttgcctga gtcagagtct
    20761 gccctttctc atttaataaa gagacttcaa ttccttgctg ggccgcgccg ttttccagac
    20821 tttgggtaaa gatatccaga cgattgtgat aacggtgagc tgaaacgccg atatcactta
    20881 acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc agcctgattc atcacagcca
    20941 gatacacgcc cgcctgctga agcggtttga tatcgcccag cggcagcaat aatttttcac
    21001 gggtgttacg cgcaggattg agatcaaacc gtccggtgta gaccagatcc gccatctgca
    21061 gcagtttgtc tgactgccag ttcgccagcg aattgcggta ttcccattgg ctaatgaatg
    21121 ctggcagaga ttctggctta acgcggaaga agttaacatc gacattatta acgttgagcg
    21181 ccattaccgg cagcccttca acgactttgc caggcagcag cgaaccacgg ctggcaaaac
    21241 cgacgctggg ttggatgtcg cgggtagtta tagttttttc gtaatcttta ctgaaggttg
    21301 cgttgttgag tgctttgacc tccttgccaa tagtaacgat caaatcacgt ttcggttcga
    21361 ggtggcgtaa acgtagctct ttaagattat ctgacagctc ccaggcacca tccactttgc
    21421 cgcttttttt atcgacgaca tgaataacgc gtgagaaatc ctgatccggg tcgagaggga
    21481 tggagaacgt cagcaccagc gtggctgcac catcaagttg gacttcagag agatccagca
    21541 atgtcagcac cttaccggca ctctgttggg ccagtttttg tctctccggc acggagagtt
    21601 ttgctgaact cgcgttttct gaagaggccg ctttagtaac ttcagaaggc gcatcttttt
    21661 tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc cagcgccagc attagcatgc
    21721 aggcggctac gcgtaacttt ttcatttttc atccctgcca caatggcccg ttagcaacgt
    21781 cgaataatta ttatgcgtga gaatttacgt tatgtaattc a
//
LOCUS       DAJTNN010000059        21545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTNN010000059 DAJTNN010000000
VERSION     DAJTNN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.059"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(126..554)
                     /gene="mxiM"
                     /locus_tag="OO150_001927"
     CDS             complement(126..554)
                     /gene="mxiM"
                     /locus_tag="OO150_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5516092.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(538..945)
                     /gene="mxiM"
                     /locus_tag="OO150_001928"
     CDS             complement(538..945)
                     /gene="mxiM"
                     /locus_tag="OO150_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5516093.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDENIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(938..1633)
                     /gene="mxiN"
                     /locus_tag="OO150_001929"
     CDS             complement(938..1633)
                     /gene="mxiN"
                     /locus_tag="OO150_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5516094.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(1575..2102)
                     /locus_tag="OO150_001930"
     CDS             complement(1575..2102)
                     /locus_tag="OO150_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5516095.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIEWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(2099..2824)
                     /gene="mxiJ"
                     /locus_tag="OO150_001931"
     CDS             complement(2099..2824)
                     /gene="mxiJ"
                     /locus_tag="OO150_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5516096.1"
                     /translation="MIRYKCFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(2830..3123)
                     /gene="mxiI"
                     /locus_tag="OO150_001932"
     CDS             complement(2830..3123)
                     /gene="mxiI"
                     /locus_tag="OO150_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5516097.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSHPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(3136..3387)
                     /locus_tag="OO150_001933"
     CDS             complement(3136..3387)
                     /locus_tag="OO150_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HCR5516098.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTSALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(3395..4510)
                     /gene="mxiG"
                     /locus_tag="OO150_001934"
     CDS             complement(3395..4510)
                     /gene="mxiG"
                     /locus_tag="OO150_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5516099.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKSGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(4519..4977)
                     /gene="ipgF"
                     /locus_tag="OO150_001935"
     CDS             complement(4519..4977)
                     /gene="ipgF"
                     /locus_tag="OO150_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5516100.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            complement(4977..5339)
                     /gene="ipgE"
                     /locus_tag="OO150_001936"
     CDS             complement(4977..5339)
                     /gene="ipgE"
                     /locus_tag="OO150_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5516101.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(5349..6965)
                     /locus_tag="OO150_001937"
     CDS             complement(5349..6965)
                     /locus_tag="OO150_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5516102.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNYLNNKNWEPINKNINY
                     NGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKELNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEILNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            7279..8763
                     /gene="icsB"
                     /locus_tag="OO150_001938"
     CDS             7279..8763
                     /gene="icsB"
                     /locus_tag="OO150_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5516103.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEV
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            8776..9165
                     /gene="ipgA"
                     /locus_tag="OO150_001939"
     CDS             8776..9165
                     /gene="ipgA"
                     /locus_tag="OO150_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5516104.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            9181..9807
                     /gene="ipgB1"
                     /locus_tag="OO150_001940"
     CDS             9181..9807
                     /gene="ipgB1"
                     /locus_tag="OO150_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5516105.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            9865..10332
                     /gene="ipgC"
                     /locus_tag="OO150_001941"
     CDS             9865..10332
                     /gene="ipgC"
                     /locus_tag="OO150_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5516106.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            10338..12080
                     /gene="ipaB"
                     /locus_tag="OO150_001942"
     CDS             10338..12080
                     /gene="ipaB"
                     /locus_tag="OO150_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5516107.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LAANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIAELLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            12100..13191
                     /gene="ipaC"
                     /locus_tag="OO150_001943"
     CDS             12100..13191
                     /gene="ipaC"
                     /locus_tag="OO150_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5516108.1"
                     /translation="MEIQNTKSAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            13242..14240
                     /gene="ipaD"
                     /locus_tag="OO150_001944"
     CDS             13242..14240
                     /gene="ipaD"
                     /locus_tag="OO150_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5516109.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSKK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKEKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            14249..16150
                     /gene="ipaA"
                     /locus_tag="OO150_001945"
     CDS             14249..16150
                     /gene="ipaA"
                     /locus_tag="OO150_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5516110.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNDLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDAVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            16154..16390
                     /locus_tag="OO150_001946"
     CDS             16154..16390
                     /locus_tag="OO150_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5516111.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            16607..17536
                     /gene="virB"
                     /locus_tag="OO150_001947"
     CDS             16607..17536
                     /gene="virB"
                     /locus_tag="OO150_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5516112.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(18480..19259)
                     /gene="ipaJ"
                     /locus_tag="OO150_001948"
     CDS             complement(18480..19259)
                     /gene="ipaJ"
                     /locus_tag="OO150_001948"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5516113.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(19859..19996)
                     /locus_tag="OO150_001949"
     CDS             complement(19859..19996)
                     /locus_tag="OO150_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516114.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            20612..21545
                     /gene="istA"
                     /locus_tag="OO150_001950"
                     /pseudo
     CDS             20612..21545
                     /gene="istA"
                     /locus_tag="OO150_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT         7287 a         4426 c         3292 g         6540 t
ORIGIN      
        1 gagccagctc aattatgtta cgttctttat cttttacaag ctcaacgtta actggttcat
       61 gtccactaga gtaaatgtaa aacatattac ttgtatttag acctttatat ttactcacaa
      121 tacaattata tccctcgaat taattttaat ttaacttccc catccttctc tataaggatg
      181 gtatcattta tcttattacc ttttaaaaca accccgtctt tcagcataga aaatagttta
      241 tctatagcta tcttttgtgc atccatgaag cacccatcta cttccttaac attaaagttg
      301 cccatgaatt tacctgcatt aaaactataa accgccttac cactaataca tttagagtaa
      361 acatttatac ttttattggt tgtattaaac gaccataata aatcatttgg aatagaaaaa
      421 taatccttac ttacaggaac aatatgccat tctttttcag aattagatga tgactttaaa
      481 gcacacccac tcagtgttaa tagcaatata cttaaaataa atattttcaa cttattacta
      541 ccatgtcgaa tcatctgcct ctctccctgc ttcaaagaca ctaattaatg aatcgaaggt
      601 tgtagtctta atatagaaat caatattgtt atatatggac aatgatgcat aaaatccctc
      661 atgcaaatcg acatattgat ttgcatctat gagtttatta agaagtgaac ttttttgtgc
      721 agctgtaaga taaggaaaac gtgaattgaa taattgttca atcttattgt gcagttcatc
      781 attatctatg gaatttaatt cactctgtga gaaatattgc attatttcat agattatatt
      841 ctcatcaaat gattgcgaag atgataatct ctcatttaaa ttaacaaatt cttcactatt
      901 taatctttgc tgcagagcat tgcttgcatt tatttgatta atcattaaca ggattctctt
      961 ttaacaagtc ttctgcaatt tgtctcagtt tcttatgtgc taactctttg aaatattttt
     1021 tatcattttt tgttaatttt tccgcaagtt ctattttaac acctgatatt acatcctgag
     1081 gactaaactc cactacttga ttaccactac agaatatata attattccct tcagaatatt
     1141 ttaaaataat cttgacatct gagcgatatt tatggagatc aagttctaac tttttcctca
     1201 gtgctaaatt acacttgggc aaaaccactt ttaattctgt tactgtatta cgcaacgaac
     1261 taagccactg ctccaataaa gtgctaatga tcctctcatc atgtaaattt tctgaaagca
     1321 aactagttat atagtcctca atatttcttc tgttttcatt ttgctttaac ttccattcac
     1381 aaatatagtc aataatatgc tcaaaagcag tctgtatacc aatttgataa ccatcgcata
     1441 cagcatctat acgcagtgat tcagcttttt tcgtagcatc ctttataaca caaattgctt
     1501 tcttcttact ctcagtgtct ctatctttta tggttttaca taattccttt tcaattctct
     1561 ttataacaac accatcatag gcatgatgtc tggataccgg taaagtgcct ttttgcatat
     1621 tgcatacctt catttaatcg ggacaaattt ctttctacag gcgtataatt tccctccaaa
     1681 gcaattggat taaataataa gtttattctt tcatatatac caacagatat attttccttc
     1741 cactcaataa gatgccaaaa accacttcga atagcggcct ccccttgctt actgattttg
     1801 cccccataat aactaataac catctcacta tgattaatta accgagactc ttgactaccg
     1861 ataaaataag caacagctgg aagtaaattc cagttggcaa caaacatagc ggttaccccg
     1921 cttaaaggtt caatatcgca atttagatca tatttgttaa ttatgagatt attaatctca
     1981 cttctgatta cgccattttc taacagttca gaaggcaaat ttaaccgatt tctatttata
     2041 tagaacgctg gatcaaaaat tattgaaaga tattttttat aaattccatc cattcttatc
     2101 atattttgtt tctcttgaac caccctgttt tgaatgccca taccaaaaga atgacaacta
     2161 gtacagccat cccgagaaat acatatatta cttcatttgt taaaaattcc gatttaattt
     2221 ccttaacagg ttgtacattt gtataaacat attcttcttt cggagttaat atgacagata
     2281 tattttcata cttaacatca gaaaaggtgt ttttcaaaaa tcgcttaata ttactaacta
     2341 atagttcaga ctctttcggt gagtcatata tagcgattac tgatatatgc atcggttttg
     2401 aagatatatt tttttcttca agatcatagc taacatgtat ttttgccgaa ataacaccac
     2461 caatagaaac taaagactgt tccagccgtt gctcaatagc actatataaa cgggcctttt
     2521 cagctcttgg agaagacact aatgaatctg taggaaacat ttgtgagata tcaactctct
     2581 ccggatttgg caaatcgtac atgcgcatca aatcaactgc cgatgcaaat gtcccctttt
     2641 cgacttgtac cgagatcccc tgtttacctc catcaacttt tctagcagta atattatggc
     2701 gttctagcac agatattatt tcatttgcct gtctttgaga taaattagaa attaactctt
     2761 cacgttgctc acatccaatc aacatcagca acaagaataa aataaaacat ttatacctaa
     2821 tcatataaat taagacttta ataaagtttc aacagccgaa actgtttttc ttgctaacgt
     2881 gccagctaat gatactccta ttgaataatt tgagagcgtc gtctgtaact tggccaaaga
     2941 ttctgggttt aatgattctg gatggcttac catctccatt atttgtgata cttgaatatc
     3001 tgtatccatc ttaatatcac tatatttagc cgacacgacg tcttccaggc ttgatatctc
     3061 ttgagattga aaatcactgg ctttgataat atcaacctga ttgactggat aaatgtaatt
     3121 catgaatgct ccctattatc tgaagttttg aataattgca gcatcaacat ccttaatcac
     3181 tttcacagta ttagattgcg cgttcctata taatgtatat tcagataatt tactttggta
     3241 ttcagccagc aactgtggat tcgaaggatt ttcagctaat gcatttagtg ccgatgttag
     3301 ttgcccttgt aatgtttgag ttccatcatc aaaagtttca gataatgaac tcagagtcca
     3361 atctttatcc ggtactgtaa cactcatttt atcctcactt atttttatct aaaaaaaacc
     3421 agtgtttatc attcaacatt acataactgt ctttgctgtt aagatatgat ttacctttaa
     3481 aatcatcatc tatcaaaagt acagaaaact caatatactg atctccatat acatttttat
     3541 gatcagaaat cagtctatta atgagctcaa gcttttcatc tgtcagttct tctctgacag
     3601 aatatgtaac tttattagtt tcacaaatct ctttatactg gacatttacc tttgtgaaaa
     3661 tacctttagc atcctccctt gcattatgat cgctaagata gttgaattta atatttttga
     3721 tatacggaaa ttcaactatc agagcatttt tcaatttctc aagttcggta tcttttgaac
     3781 tatttctttc tttacttaaa aataccagta attccgcttt atcactcact aaattcaaca
     3841 catacaaatt gatatatggg aatcgttgat tgatatattg ttgaattcta tttatttctt
     3901 tgttggacac cagaatatat ctgcatttgg taaatccgct tttatttaag gctacagatg
     3961 cccataccgc atcattttgt gtttcggcaa gaatataatt gcactgtcct gtgttgacta
     4021 ttccatatct cttatcatct ataatttcag caatttcttt tacttcatct ttctttaaag
     4081 agagggaaaa aattataatt aacacaacaa taacagcaaa gaaaaaaaac accgagggtt
     4141 tattatacat tccgtttagt atatgaactc tcgatttatc ctccatcata tttttaagtt
     4201 gaaaagaaac tccgtcatat tcaaatgagt taaattgtga attgatatca atttgttcac
     4261 ctttttcatc actaaattga cagatattat ctttacttat ggccaaataa attataccat
     4321 ccgatttact atctgttact ggaataatat tttctgggaa attatcgttt ccaaactcaa
     4381 gtgtttctat cgtccgtccg agaacaatta agttattccc ataatataac ggaaactcat
     4441 ccccgacacc gtttgtgagt ttcactaata acctgaatgg tgcaagattt gagttctttg
     4501 cctcagacat aaatcctctt atatccttcg attattctgc ttgctctctg ctgctatccg
     4561 caaatagcgt ttatagatat cctctgcgta ctttaatctt tcttttttct ttttcggaga
     4621 tgttcctgca ttataggcac caacagcctc ccaccctctt ccatacatca gcataaactc
     4681 atttaataac tttgctgcat aatgtacaga aagacatgga tgactaatta gcatttcctc
     4741 agtatatccc atttctctaa gtcttttaga atgaaagtca tttatttgca ttataccata
     4801 atctttactt ccattgttat taacattgac tgcaaattta ttaaatccgg attctttttc
     4861 cgcaatcgct tttaacaaac tcgatggaat gttgtacctt tcaccagcct tatcccaaca
     4921 atcagctctc cctaaatatg ggataaaaca cagaaaaata aatacgaaac gggacattaa
     4981 taccccttca ttcttcgcgc aaattcatcc ttcagccaac gcacacgcga aatataatat
     5041 tctgtattaa catatatatc ctcgaattca tttatcccgg taagatctaa tcgcgcgatt
     5101 agacttccac cttcatcatc agtagctaaa catatctttt ctgagtaatt taggctcaac
     5161 gcatatataa gatcatttat attttcaggc aatgcacaaa aaggacataa taaatttatt
     5221 gaatctcctt ctttttcaat atatattaac acatcatcat caagcatgat tgcttgatca
     5281 tcggtatcaa ttgaagggat acccaaggct cggcaaataa catctgctaa atcttccata
     5341 tattcctctt atacaaatga cgaataccct ttcaccatat tccatatttt ggggtcccct
     5401 attctttcag cataagacag ctctaacgaa gatagcggta attttttcat aactttattt
     5461 ccaggcacac cagtattcat ctcttggatt tccatattac cactattcat tagaatagta
     5521 gaaaataatc ttttctcttc tgaggataat ttactattta attgagaaaa ctgccctgtt
     5581 tcatgttttc ttattatttc tcttttaatt tcagcatcct gcatacctgt tctgtccttc
     5641 ccactcttac aattccagca aggtacagca cctatagtat aagccaatag agccattctt
     5701 tgtgacaatt tatacggctc tccattatca tttttttgta acttcttgtt tagaatctct
     5761 tttatctggt tagccagata tatcacatca ttcttacatg gtggattttt ttctatagct
     5821 tctgcagccc aacctccaat cacaccattt ttgaggaaat tatcccccaa caaagaacat
     5881 attgattcac cattaagctt gtcaacattc ctccagccta agcccatttt tagcgctaat
     5941 tcatttacac caaagttaaa tgtcaccact tttagattaa cctttacttc ttgcagaaga
     6001 ccatcactat tccgaattaa taatttcgtt ggttcccccc ttttagagtt taactctttt
     6061 aatgccttta cctgatcttt taacatactc tcctctcccc cagttaaact ggtcggcgtc
     6121 agaagagaag tcgaaacaat ctttaaatct actgttttac cagacaaagc ctgtgataat
     6181 aattcttgtc tgctatataa tgctgcgctt gctaactcct cagctttatt acgagctgca
     6241 acagctcttt cagatgagtt ttttttcaat ccataggctg atataactcc atgtctgata
     6301 cccgaaaaaa tttccttccc ctcgtcatcc accacttttg aaagccacat attcgcaata
     6361 tgcttactct ctctcgtaga agcacaacaa attccttttc cattatactc tttgacaaat
     6421 atatttttat tccctatctt catatgagag gcgggtgtta attgaaaacc ataatttttc
     6481 ccattataat taatattttt gttgatcggt tcccaattct tattatttaa ataattagta
     6541 aatatttgtt ttatttccga ttgcaattcc tttttatttt taatgcctag ctgtgctctt
     6601 atctcttcac ttattacttt attaagctca ttaatatttc ttttatcaac ttcaccaagt
     6661 ttataggtat ctaattgttc catcatggct ttaatttttg acgtatcacc acctgccaac
     6721 tttattgcct cgtaaaaaac tgcagctctg tcacgcgcaa acaaaaggcc tttccctgtt
     6781 aagcttgttt ggttctgtaa atataatcga ttaagcgctt cgaccccctt atttctctct
     6841 ccatttttaa ctctttgata tgaaatcacg cttacacctt tttggtttcc ggtttcttct
     6901 gcgcctttat aggaatctcc gctttgaaat gaaacctgat gcaatcccaa attagttatg
     6961 tgcatttaat tatcctcagt cataaatggg gcattgattg cagttttgta cctcgtttta
     7021 caacaaaaaa gatacttatt caacagggtt ctctcttttt ctataacact ccgagcctga
     7081 tatatcagac actttaactt gtgggatttc atgatgaaac cagcactaca tatgctcacg
     7141 aggtacatat tatgtacagc aaaagaaaat ttgattatgt taacaggctc aagtttctaa
     7201 atttattaat tgaatatatt atatatcaaa tacattttac atccccacaa tcaccacgta
     7261 atggagagtt aataaagtat gattctcaaa attagcaatt tcattgacgc aagcaataca
     7321 aaagggccta tacgcgttga agatacagag catggaccta tattggtagc acagaaattc
     7381 aacctaaagg atctcttttt cagaacatta agcactatta atgcaaagat aaattcacag
     7441 atattaaatg aacaactaaa aaattatcga ctagcgaatc aaaaatcctt attgcttttc
     7501 ctcaagacat tagctagcga aaaatctgca gaaagtgcgt ttgccgccta tgaagcagtc
     7561 aaaaattcaa ttcaacattc ttttacaggt aaagacatca aattaatgtt gaataccgca
     7621 gagagttttc atggcatcgg tacagccaaa aatcttgaaa gacatttagt gtttcgttgc
     7681 tggggaaata gaggcataac ccatttaggg cataccagta tcagtataaa aaacaattta
     7741 ctacaggaac caactcatac ctatctcagc tggtatcccg gcgggaatgt tacaaaagat
     7801 acagaaataa actacctttt tgaaaaacgc tcaggttaca gtgtcgatac ctataaacaa
     7861 gacaaactaa atatgatttc agaccaaaca gccgaaagac ttgatgcagg ccaagaggtt
     7921 agaaacttat taaattcaaa acaagatcaa aataacaaca aaaaaatatt ttttcccaga
     7981 gctaatcaaa aaaaagaccc ctatggttat tggggggtca gtgctgataa agtatacatt
     8041 ccattatcag gtgataataa aacaaaggat ggtaaaatca gctataacct ttttggcctt
     8101 gatgaaacaa acatgtcaaa atttatatgc caaaagaaag cagatgcttt cagacaattg
     8161 gcaaattata aattaatcag taaatctgaa aactgtgctg gtatggcact taatgttctt
     8221 aaagcaggaa attctgaaat atactttcca ctccctgacg ttaaattggt tgctacacct
     8281 aacaatgtat acgcgtatgc aaataaagtt aggcaacgca ttgaaagtct caatcagtct
     8341 tataacgaga taatgaagta tattgaatct gattttgatc tttccagatt gactcaatta
     8401 cgacgtagct atcttaaaag tttcaataaa attaacctta tccacacccc aaaaacattc
     8461 aagcctttat caatttcact ttacaaacat cctactgaaa atgtatcttc agaagacttt
     8521 gatgcggtca tcaacgcttg ccactcatac ttagtaaaat cagcacccag caatatgtct
     8581 cgtgtattaa acgaattaaa aaccggagca acagataaaa aagaagtaat tatcgaaaag
     8641 tcaataaaaa ctattgatta ttacaattct cttaaatcgc cagatcttgg aaccaaactt
     8701 tacattcacg acttattaca ggtcaataag cttttattga ataactctca ttctaatata
     8761 tagaaggcca tagaaatgtg tcgcaaacta tatgataaac tttatgaaat aacaggtgca
     8821 aagcttgatt ttaacgataa aaatcaagcc tttatattgc tcgaagaaca aatacctgtt
     8881 tgtataacag ataatgatga atacatcttt ttgaccggac ttcttaatga acatgaactt
     8941 tttacagaaa atattattaa tccagaacac atactcatcc ttaactactc tttgagtaga
     9001 gattatggtt cttctatctg tcttctccct gatactcatc aatgtgtttt gaccaagaaa
     9061 cactacaaaa aatatctttc acctgatgaa ctcattgaat ctttgtacga atttttattt
     9121 tgtattaaac taaccattgc aaacatcact tcagaagtga actaacatat agggggtatc
     9181 atgcaaattc taaacaaaat acttccacag gttgaattcg cgattcctcg cccatcattt
     9241 gattcgttaa gtcacaataa gcttgtaaag aaaatattat ctgtttttaa cttaaaacaa
     9301 cgctttcctc agaagaattt tggatgcccg gtgaatataa ataaaatcag ggacagcgtc
     9361 atagacaaaa taaaagatag taattcgggg aatcaactat tttgttggat gagccaagag
     9421 cgaacctctt atgtctcttc aatgataaac agaagtatag atgaaatggc catacacaat
     9481 ggagttgttt tgacctcaga taacaaaaaa aacatatttg cagctattga aaaaaaattc
     9541 ccggatataa aacttgatga aaaatcagca caaacatcta tcagtcatac agcactaaac
     9601 gagattgcct catctggcct cagagcaaag attttaaaac gttatagcag tgacatggat
     9661 ttgtttaaca cacaaatgaa ggatcttaca aatctagtat ccagttccgt ctacgataaa
     9721 atatttaatg aatcaacaaa agttttacaa atagaaataa gcgcagaggt tctaaaggct
     9781 gtataccgtc aaagcaatac aaattaaatc tgtttatatt aaatcttata cttattaaag
     9841 taatgcagac aaaaaggaga ccttatgtct ttaaatatca ctgaaaatga aagcatctct
     9901 actgcagtaa ttgatgcaat taactctggc gctacactga aagatattaa tgcaattcct
     9961 gatgatatga tggatgacat ttattcatat gcttatgact tttacaacaa aggaagaata
    10021 gaggaagctg aagttttctt cagattttta tgtatatacg acttttacaa tgtagactac
    10081 attatgggac tcgcagctat ttatcagata aaagaacagt tccaacaagc agcagacctt
    10141 tatgctgtcg cttttgcatt aggaaaaaat gactatacac cggtattcca tactggacaa
    10201 tgccagcttc ggttgaaagc ccccttgaaa gccaaagagt gcttcgaact cgtaattcaa
    10261 cacagcaatg atgaaaaatt aaaaataaaa gcacaatcat acttagacgc aattcaggat
    10321 atcaaggagt aattattatg cataatgtaa gcaccacaac cactggtttg cctcttgcca
    10381 aaatattggc ttccactgag cttggagaca atactatcca ggctgcaaat gatgcagcta
    10441 acaaattatt ttctcttaca attgctgatc ttgctgctaa caaaaatatt aatacaacta
    10501 atgcacactc aacttcgaat atattaatcc ctgaacttaa agcaccaaag tcattaaatg
    10561 caagttccca actaacgctt ttaattggaa accttattca aatacttggt gaaaaatctt
    10621 taactgcatt aacaaataaa attactgcct ggaagtccca gcaacaggca agacagcaaa
    10681 aaaacctaga attctccgat aaaattaata ctcttctatc tgaaactgaa ggactaacca
    10741 gagactatga aaaacaaatt aataaactaa aaaacgcaga ttcaaaaata aaagacctag
    10801 aaaataaaat taaccaaatt caaacaagat tatccgaact cgccccagat tcaccagaaa
    10861 ataaaaaatt aagacgggaa gaaatacaac tcactatcaa aaaagacgca gcagttaaag
    10921 acaggacatt gattgagcag aaaactctgt caattcatag caaacttaca gataaatcaa
    10981 tgcaactcga aaaagaaata gactcttttt ctgcattttc aaacacagca tctgctgaac
    11041 agctatcaac ccagcagaaa tcattaaccg gacttgccag tgttactcaa ttgatggcaa
    11101 cctttattca actagttgga aaaaataatg aagaatcttt aaaaaatgat ctggctctat
    11161 tccagtctct ccaagaatca agaaaaactg aaatggagag aaaatctgat gagtatgctg
    11221 ctgaagtacg taaagcagaa gaactcaaca gagtaatggg ttgtgttggg aaaatacttg
    11281 gggcactttt aactatcgtt agtgttgttg cagcagcttt ttctggagga gcatctctag
    11341 cactggcagc tgttggttta gctcttatgg ttacggatgc tatagtacaa gcagcgaccg
    11401 gcaattcctt catggaacaa gccctgaatc cgatcatgaa agcagtcatt gaacccttaa
    11461 tcaaactcct ttcagatgca tttacaaaaa tgctcgaagg cttgggcgtc gactcgaaaa
    11521 aagccaaaat gattggctct attctggggg caatcgcagg cgctcttgtc ttggttgcag
    11581 cagtcgttct cgtagccact gttggtaaac aggcagcagc aaaacttgca gagaatattg
    11641 gcaaaataat aggtaaaacc ctcacagacc ttataccaaa gtttctcaag aatttttctt
    11701 ctcaactgga cgatttaatc actaatgctg ttgccagatt aaataaattt cttggtgcag
    11761 cgggtgatga agtaatatcc aaacaaatta tttccaccca tttaaaccaa gcagttttat
    11821 taggagaaag tgttaactct gccacacaag cgggaggaaa tgtcgcttct gctgttttcc
    11881 aaaacaacgc gtcgaaaaat ctagcagacc tgacattatc gaaatatcaa gttgaacaac
    11941 tgtcaaaata catcggtgac gcaatagaaa aattcggcca attgcaggaa gtaattgcag
    12001 agctgttagc ctcaatgtcc aactctcagg ctaatagaac tgatgttgca aaagcaattt
    12061 tgcaacaaac tactgcttga tacaaataag gagaatgtta tggaaattca aaacacaaaa
    12121 tcagccccga ttttatatac agatatatct acaaaacaaa ctcaaagttc ttccgaaaca
    12181 caaaaatcac aaaattatca gcagttagca gcacatattc cacttaatgt cggtaaaaat
    12241 cccgtattaa caactacatt aaatgatgat caacttttaa agttatcaga gcaggttcag
    12301 catgattcag aaatcattgc tcgccttact gacaaaaaga tgaaagatct ttcagaaatg
    12361 agtcacacaa ttaccccaga gaacaccctg gatatttcca gtctttcttc taatgctgtt
    12421 tctttaatta ttagtgtagc cgttctactt tctgctctcc gcactgcaga aactagattg
    12481 ggctctcaat tgtcattgat tgcgttcgat gctacaaaat cagctgcaga gaacattgtt
    12541 cggcaaggcc tagcagccct atcatcaagc attactggag cggtcacaca agtaggtata
    12601 acgggtatcg gtgccaaaaa aacgcattca gggattagcg aacaaaaagg agccttaaga
    12661 aagaaccttg ccactgctca atctcttgaa aaagagcttg caggttctaa attagggtta
    12721 aataaacaaa tagatacaaa tatcacctca ccacaaacta actctagcac aaaaatttta
    12781 ggtaaaaata aactggcacc agataatata tccctgtcaa ctgaacataa aacttctctt
    12841 agttctcccg atatttcttt gcaggataaa attgacaccc agagaagagc ttacgagctc
    12901 aatacccttt ctgctcagca aaaacaaaac attggccgtg caacaatgga gacatcagcc
    12961 gttgctggta atatatccac atcaggaggg cgttatacat ctgctcttga agaagaagaa
    13021 caactaatca gtcaggccag cagtaaacaa gcagaggaag catcccaagt atctaaagaa
    13081 gcatcccaag cgacaaatca attaatacaa aaattattga atataattga caacatcaac
    13141 caatcaagga attctacagc cagtcagatt gctggtaaca ttcgagctta atatatccaa
    13201 gagccataat aatatatggc tcttcctata aggaaataac catgaatata acaactctga
    13261 ctaatagtat ttccacctca tcattcagcc caaacaatac caacggttca tcaaccgaaa
    13321 cagttaattc tgatataaaa acaacgacca gttctcatcc tgtaagttcc cttactatgc
    13381 tcaacgacac ccttcataat atcagaacaa caaatcaggc attaaagaaa gatctctcac
    13441 aaaaaacgtt gactaaaaca tcgctagaag aaatagcatt acattcatct cagattagca
    13501 tggatgtaaa caaatccgct caactattgg atattctttc caagaaagaa tatccaatta
    13561 ataaagacgc aagagaatta ttacattcag ccccgaaaga agccgagctt gatggatatg
    13621 aaatgatatc tcacagagaa ctgtgggata aaattgcaaa gtcaatcaat aatattaatg
    13681 aacagtatct gaaagtatat gaacatgccg ttagttcata tactcaaatg tatcaagatt
    13741 ttagcgctgt tctttccagt cttgccggct ggatctctcc cggaggtaac gacggaaact
    13801 ccgtgaaatt acaagtcaaa tcgcttaaag acgaattgac aaagctcaag gaaaaatata
    13861 aagataaacc gctatatcca gcaaataata ctgttagtaa ggaacaagca aataaatggc
    13921 ttacagaatt aggtggaaca atcggcaagg tatctgaaaa aaacggggga tatgttgtca
    13981 atataaacat gaccccaata gacaatatgt taaaaagctt agataatcta ggtggaaatg
    14041 gcgaggttgt gctagataat gcaaaatatc aggcatggaa tgccggattc tctgccgaag
    14101 atgaaacaat gaaaaataat cttcaaactt tagttcaaaa atacagtaat gccaatagta
    14161 tttttgataa tttagtaaag gttttgagta gtacaataag ctcatgtaca gatacagata
    14221 aactttttct ccatttctga ggtgcgccat gcataatgta aataatactc aagcgccaac
    14281 attcttatat aaggcaactt caccatcatc aacagaatac agcgagttaa aaagcaaaat
    14341 atccgatatc catagttcgc aaacttctct aaaaacacca gcatcagtgt ctgaaaaaga
    14401 aaactttgca acgtctttta atcagaaatg tcttgatttt ttattttctt cctcagggaa
    14461 agaagatgtg ttaagaagca tttattccaa ctcaatgaat gcgtatgcca aaagcgagat
    14521 tctcgaattt tcaaatgttt tgtactcctt agtacatcaa aatgatctta attttgaaaa
    14581 cgaaaaggga cttcaaaaaa ttgtcgcaca gtattcggaa ctaattataa aagataaatt
    14641 atcccaagat tctgcctttg gaccatggtc ggcaaagaat aagaaactcc atcaattacg
    14701 acaaaacatt gagcacagac ttgcactatt agcacaacaa cacacatctg gtgaagcttt
    14761 atcattggga caaaaactcc tcaatactga agtatcatca tttatcaaga ataatattct
    14821 tgctgaatta aagttaagta atgaaactgt ttcatctctc aaactagatg atttagttga
    14881 cgcacaggca aaacttgcct ttgatagttt gcgcaatcaa cgtaaaaata ctattgatag
    14941 taaaggattt ggtataggta aactgtcaag agacttaaat acagtagccg tgtttcctga
    15001 gctgttgaga aaagtcctta atgatatttt agaagatata aaagattcgc atcctatcca
    15061 agatggcctc cctacacctc ccgaagatat gccagatggc ggaccaaccc ccggagccaa
    15121 tgagaaaaca tcccaacctg taattcacta tcatataaat aatgataata gaacttacga
    15181 taatagagtt tttgacaaca gagtatatga caatagctat cacgagaacc cagaaaatga
    15241 tgcacagtct cctacttctc agacaaacga tctattatcc cgtaacggaa actcattact
    15301 aaatccacaa agagcactag ttcaaaaagt aacttccgtt ctaccacact ctatatcaga
    15361 tgctgtccag acatttgcaa ataattcagc tttagaaaag gttttcaacc atactccaga
    15421 taattcggat ggaataggtt cagacctgtt aactacgagt agtcaagaaa gatctacaaa
    15481 taactctctt tctcggggac acaggcctct gaacatacag aactcttcaa ccaccccccc
    15541 tctccacccg gaaggagtga caagcagtaa tgataactca tcagatacaa ctaaaagtag
    15601 cgcttctctt tctcatagag tagcttcgca aatcaataaa ttcaactcaa acactgattc
    15661 aaaagtactt cagactgatt ttttatcaag aaatggagac acatatttaa cacgggaaac
    15721 gatatttgaa gcttcaaaaa aagtaacaaa ctccctaagt aatcttatat ctctcattgg
    15781 aactaaatca ggaacacaag aacgagagtt acaggaaaaa tcaaaggaca ttacaaaatc
    15841 cacaacagaa cacagaataa acaacaaatt aaaagttaca gatgcaaata caataaacta
    15901 cgtaacagaa accaacgcag atacaattga taaaaatcat gcgatctatg aaaaggcaaa
    15961 agaagtatct agcgccctca gcaaggtatt gtcaaaaatt gacgatacct ctgcagaatt
    16021 acttacagat gatatatctg atttaaaaaa taacaatgat attacagctg aaaacaataa
    16081 tatatataaa gcagcaaaag atgtaaccac ttccctatca aaagtattaa agaatatcaa
    16141 taaggattaa aatatgataa aagaaaaaat attatcaata gtggcctttt gctatggtat
    16201 agcgtacagc aaactatctg aagaaactaa atttattgaa gatctttctg ccgattctct
    16261 ttctctgatt gaaatgctgg atatgattag ttttgagttc aatctacgta tagatgaatc
    16321 tgcattagaa cacattatta ctattggaga tcttatttct gtagtcaaaa atagtacaaa
    16381 atcaatatag taattgataa gcattttttc atctatggag ctctcacatc agagctccac
    16441 aagaatatta ttcttttatc caataaagat aaattgcatc aatccagcta ttaaaatagt
    16501 aatttttaag actaccgttg actatcatca acagttacat acaatcttgc tcacactgca
    16561 tttaactttt gtcaatataa atcaatatga ataaacaggg tgtgatatgg tggatttgtg
    16621 caacgacttg ttaagtataa aggaaggcca aaagaaagag tttacactcc attctggtaa
    16681 taaagtttcc tttatcaaag ccaagattcc tcataaaagg atccaagatt taaccttcgt
    16741 caaccaaaaa acgaatgtac gcgatcaaga atccctaaca gaagaatcat tagccgatat
    16801 cataaaaact ataaagctac aacaattctt ccctgtaata ggaagggaga ttgatggtag
    16861 aattgaaatt ctggatggca ctcgtagaag agcatctgca atatatgcag gagcagatct
    16921 tgaagttcta tattcaaaag aatatatatc tactcttgat gccagaaaac tagcaaacga
    16981 tatacaaaca gcaaaagagc atagcatccg agaacttggt attggtctta attttctgaa
    17041 agtatcaggg atgtcctata aagacatagc caaaaaagag aatctgtctc gcgcgaaagt
    17101 cactcgtgcc tttcaggcag caagcgttcc acaggaaata atatctctat ttccaatcgc
    17161 gtcagaactt aactttaatg actacaagat attattcaat tattataaag gacttgaaaa
    17221 ggctaatgaa tctcttagtt ctacactacc aatattaaag gaagaaataa aagaccttga
    17281 tacaaatttg cccccggaca tatataaaaa agaaatttta aacatcataa agaaaagcaa
    17341 aaacagaaag caaaaccctt cgctaaaagt tgactcgtta tttatttcta aagacaaacg
    17401 gacttacata aaaagaaaag agaataaaac aaacagaact ctaatattta cattatctaa
    17461 aataaacaaa acagttcaga gagaaataga tgaagctatt cgagatataa tttctcgcca
    17521 tctatcgtct tcataaacat cgaacactga tgtaaactgc ccaattcaga taataaaaat
    17581 cttcgttgaa cttgactttc agccggtggg gtaattccgt cggctatctg ttattcaatc
    17641 cccacgcact gtgtttcctt aaaattacgg tgaaaactca taccctgctg accacgagaa
    17701 cattatctgg tgaaattagt aatattcata tcattgaaaa tgaattgtgc aactaaggga
    17761 tgcggcatga tggtcaaaat tttatgtgaa gatgagtgac tggccgaaaa tattcagcgt
    17821 atttttaatc gtttcaataa aaacagcgag gattagcagt caacgctttt actactcaat
    17881 tttttacatg gtgataaaca aaaaatccac cattaacaga cttcgttcac agtatcaaaa
    17941 aagctttaag aatatattag cagatttctc aaatataatt actgttttat tctcctaatc
    18001 ttcccataaa atgaccgact tacatgattt tataattata aacaagtttg attttaaaat
    18061 catattaatt attaacaaga gatttatttt tctaacccca aaagttatgc ttagtcaaag
    18121 agtaacttat aatatccata cccctcatag ggcttacagt ttaaattgcg ggtataaaat
    18181 acatctattt tcaaataaaa taaagcatac tccaattaca acgagtgact aaaataaaaa
    18241 tatcatccaa gtcaatcact gtttgctctg caaaatatgt cagccttctc aaacttacca
    18301 gcacttaaat atttaagcac acaatcattc cttgagggag ttcacttatt tcaagagaaa
    18361 catttcgtct tcccacacaa ctaatccgcc caatatagct aaatttgtac tcagtaatat
    18421 catttgttat gcccccgccc caaaatattc aagctccttc acagataaaa aacgacaatt
    18481 tacaaagcct cattagttat aactatggaa atacctattt tagtatatcc tataaagtta
    18541 gaattacttc ttcttgcacc taattccata gttgaaaaac aagaataatt ttctcctaca
    18601 gctggatcca tatagcttcc atcaggtcta cacaacaccc agtggaggcc gacaggaact
    18661 ccaataaagg atacagcaac agcttccaaa actctttggt tagaagataa cacatctcgt
    18721 tgatgtacta tattacatcc catgccaatt aataagtccc ttgcatcggg ataaataaaa
    18781 ctaattactt gcgaaaaaat gttactttct tcaacgacat aagcattcaa tccaagaagc
    18841 ctcgtagcca tgactatctt atcaggcata gaataaccgg catccgcgat attatctttg
    18901 tgtattctcg gattataatt accactagtt attaagtata aatcccgctc acaacgatta
    18961 tccaaatcaa gagaactctt ccttgtcatt tcggacatag accctttgta ttgtggtatt
    19021 ttatcaaccc ctaattcctt tgcagcacag agtaaactgg cagctccaca agaattgtca
    19081 tacatctgct tcctcgccaa aaaacaacct tgttcgttta ctctattctc atttaccctc
    19141 acatcagtct gatgagatat catatattct cttggtatag ccccctgcaa taaaacccca
    19201 tataacataa cacctgtatg aatacatccc cgtttgcaag gcttccgttg ttccgacatc
    19261 gacgagtaat tgcagcacag tattgcatcc agcaatgcga actactatga gaattagctg
    19321 accgggtcct tattccccga acgaacttta aatccaaaac ctcatactct tttccgtcgt
    19381 catcacgtcc gtcaccataa caacctttca gcatccaccc ttttattaag agttaatgtc
    19441 catcagcatc agcaattaat accgcctgat actgacatat ttttgtcgta tttacgtcca
    19501 taatcggtta ttgtgataca ggtcacacca gcttatttgc tattgttaca ataaccacct
    19561 tggttgagag ctgttttttc aactttgtta tcaactcccc caactccttg gtcagtaagg
    19621 ctgccgaccg agcacagtga atagatagta tccgcaggta ccaccatgat tacttaatac
    19681 tcagaaggcg aagtgctctc caattaactc tgccgcacta tgcttataag ctgcttttca
    19741 atgccgtcta tcaacatgtc cagttcattc aatctgttgt actggtaatc caacgcagta
    19801 atgatatatg caggtattct ttcatccact ccagtaacgt ttcatgcaaa gcattaattt
    19861 acgcgctacg gaacttcacc agttgactga gcaaacgatg tgggaccagt actgcctgca
    19921 gttcttctat tgttacagcg atttctttac caaacagctg aaccaccatc aagataaaga
    19981 tagcctgaac attcatcgca ccattttgtt accaatggca aaagatttaa cgaaccgtgc
    20041 ctgcatcagg cggacttttt atccctgctt tgttagctcc ctaaccttgt acagagagcc
    20101 tccataagct cccatatcaa gcagacatgg ttcgcagtta ctgacataca ccagaaaact
    20161 ctgacagcag actcacttat cctccacttc actcatatcc ttattggtga gatgaacctg
    20221 aatgacatgt tttgcgacat aaacgccaac cagtgtatat ttcacctgta gagattctac
    20281 aatctgtagc gttatgcatc taatgttagg ctctatgatg tcagaaatct gcgagactct
    20341 acaccttctg catttacctt tatttatcac catgctcctc agttaacgga gaacgtgttc
    20401 attgcatttt gtcacttaca tttcgccttt ggtatcgggg ggatataggt tccaacgttc
    20461 cgcatgagaa cctgaaccgg cttcacgcca ccttgatacc agatgccgca caggcagtca
    20521 acaagtgtca cgaacggtgc aatagtgatc cacacccaac gcctgaaatc agatccaggg
    20581 ggtaatctgc tctcctgatt caggagagct tatggtcact tttgagacag ttatggaaat
    20641 taaaatcctg cacaagcagg gaatgagtag ccgggcgatt gccagagaac tggggctctc
    20701 ccgcaatacc gttaaacgtt atttgcaggc aaaatctgag ccgccaaaat atacgccgcg
    20761 acctgctgtt gcttcactcc tggatgaata ccgggattat attcgtcaac gcatcgccga
    20821 tgctcatcct tacaaaatcc cggcaacggt aatcgctcgc gagatcagag accagggata
    20881 tcgtggcgga atgaccattc tcagggcatt cattcgttct ctctcggttc ctcaggagca
    20941 ggagcctgcc gttcggttcg aaactgaacc cggacgacag atgcaggttg actggggcac
    21001 tatgcgtaat ggtcgctcac cgcttcacgt gttcgttgct gttcccggat acagccgaat
    21061 gttgtacatc gaattcactg acaatatgcg ttatgacacg ctggagacct gccatcgtaa
    21121 tgcgttccgc ttctttggtg gtgtgccgcg cgaagtgttg tatgacaata tgaaaactgt
    21181 ggttctgcaa cgtgacgcat atcagaccgg tcagcaccgg ttccatcctt cgctgtggca
    21241 gttcggcaag gagatgggct tctctccccg actgtgtcgc cccttcaggg cacagactaa
    21301 aggtaaggtg gaacggatgg aacagttttt acatcccact aatgactcgc ctgcgcccga
    21361 tggggagcac tgtcgatgtt gaaacagcca accgccacgg tctgcgctgg ctgcacgatg
    21421 tcgctaacca acgaaagcat gaaacaatcc aggcccgtcc ctgtgatcgc tggctcgaag
    21481 agcagcagtc catgctggca ctgcctccgg agaaaaaaga gtatgacgtg catcctggtg
    21541 aaaat
//
LOCUS       DAJTNN010000060        20755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTNN010000060 DAJTNN010000000
VERSION     DAJTNN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.060"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(11..556)
                     /gene="hemG"
                     /locus_tag="OO150_001951"
     CDS             complement(11..556)
                     /gene="hemG"
                     /locus_tag="OO150_001951"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5516115.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OO150_001952"
     CDS             complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OO150_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5516116.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2058..2672)
                     /locus_tag="OO150_001953"
     CDS             complement(2058..2672)
                     /locus_tag="OO150_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5516117.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGIGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OO150_001954"
     CDS             complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OO150_001954"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5516118.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4193..6382
                     /gene="fadB"
                     /locus_tag="OO150_001955"
     CDS             4193..6382
                     /gene="fadB"
                     /locus_tag="OO150_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5516119.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6392..7555
                     /gene="fadA"
                     /locus_tag="OO150_001956"
     CDS             6392..7555
                     /gene="fadA"
                     /locus_tag="OO150_001956"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5516120.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCN
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OO150_001957"
     CDS             complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OO150_001957"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5516121.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OO150_001958"
     CDS             complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OO150_001958"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5516122.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10154..10642
                     /gene="rfaH"
                     /locus_tag="OO150_001959"
     CDS             10154..10642
                     /gene="rfaH"
                     /locus_tag="OO150_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5516123.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OO150_001960"
     CDS             complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OO150_001960"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5516124.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELASQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OO150_001961"
     CDS             complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OO150_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5516125.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OO150_001962"
     CDS             complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OO150_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5516126.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OO150_001963"
     CDS             complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OO150_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5516127.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OO150_001964"
     CDS             complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OO150_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5516128.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OO150_001965"
     CDS             complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OO150_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5516129.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OO150_001966"
     CDS             complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OO150_001966"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5516130.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OO150_001967"
     CDS             complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OO150_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5516131.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OO150_001968"
     CDS             complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OO150_001968"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5516132.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18806..19618
                     /locus_tag="OO150_001969"
     CDS             18806..19618
                     /locus_tag="OO150_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5516133.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGMHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            20012..20672
                     /gene="hcp"
                     /locus_tag="OO150_001970"
                     /pseudo
     CDS             20012..20672
                     /gene="hcp"
                     /locus_tag="OO150_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680647.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5081 a         5603 c         5211 g         4860 t
ORIGIN      
        1 ttctttatgc ttatttcagc gtcggtttgt tggttaactg ggcgatttct cgggcgaaat
       61 tcgccacctg ctcccaatcg gtatagacaa cttctttgcg cgtatccgtt tcaccgcctg
      121 acatcttcat aatcagctta atcataaaac ggtcgtacca gcgataacgt gggtaacgca
      181 gcgccccggc aatgaccgcg cagcgatcgg gacgccattg tgaactcatc agaaacttgc
      241 gcgcgtagct gttggtctgt ggagtacgct tctccggttt gcgcgccacc aggtttacgg
      301 aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg ttttttgaca aattcctgga
      361 acgcagagtg gtagtgacca tagcgaatag aagcaccaat gaccacacgg tcatagtttt
      421 cccattgtgg ttcttcaatg cggtgcacat tggcgacatc cgtctggatc cccagttctt
      481 tcagttccga agccaggtag gaggcaatct cgcgcgtttg tccgtccctt gttgagaaaa
      541 gaattaatgt tttcacgtat tactccatca ttcacgccag aaagtcgggg taaagagcac
      601 cagcaatgta aagacctcga gacgaccaaa cagcatgttg gcaatcagga tccatttagc
      661 caccgggttc atactggtaa agttatcagc aaccacgcca agccctggcc ccaggttatt
      721 caatgtcgca acaactgagg caaaggcaga aaaatcatcc acgcccgtgg cgataatcgc
      781 cagcatactg acaataaaca ccaatgcata ggcggagaaa aatccccaaa cggcttcgag
      841 gatacgttcc ggcagtgcgc gattccccag cttaatgcta tagacggcgt tcggatgcac
      901 cagtcgtttc agctcacggt tcccctgctt aaacagcagc aggatgcgga tcactttcag
      961 gccaccgccc gttgacccgg cgcaaccgcc gataaaagct gaacataaaa gcagtaccgg
     1021 caaaaagagc ggccagcggg caatgctgtc agtcgtaaac ccggcggttg tcgccatcga
     1081 taccacctgg aaaaacgcct ggttaattgt catcagcgcc gaactgtaga cattatgaaa
     1141 ccacagtacg agtgtacaaa taaccaccag cgtaaactgt acgccgataa acatgcgaaa
     1201 ttccggatcg cgccaataaa ccttcagact acgcccactt aacagtgaaa agtgcagacc
     1261 gtagttacaa ccggagatca gcaggaagat agcaatgata gtgttaatag tcggactgtc
     1321 gaaataaccg atactggcat catgtgtcga gaagccgcca atagcgatag tcgcaaagct
     1381 atggccgatg gcatcaaagg catccattcc ggcaaaccac aacgccagcg cacaggcgac
     1441 ggtcagcaag acataaatca accacagggt tttcgccgtt tccgcaattc gcgggcgcat
     1501 tttgttatct ttcagcgggc cgggcatttc tgcgcgatag agctgcatcc cacccacgcc
     1561 gaggataggc agtatcgcaa ccgctaacac gatgatcccc atcccgccaa accattgcag
     1621 catctggcga taaaagagga tggcatgagg gagcgaatcc agccccacca gcgtagtggc
     1681 cccggtggtg gtcaggccag agaaagattc aaaaaacgca tcggtaatcg tgaggttcgg
     1741 gctttccgag aagataaaag ggagcgcacc gacactaccc agcacggtcc aaaacagcac
     1801 cactatcaga aacccctcac gggatttcag ttcgcccttc tctttgcggt tcggccacca
     1861 cagcatagag ccaatggcga gggcgacaaa aaaggtctgg gtaaaagcgc ggcccgctcc
     1921 atcccggtag atgagtgcta ccagcccagg gataatcatg gtccctgaaa ataagatgac
     1981 cagtagtcca acgattcggg taatggcgcg aaaatgcatc tctgccgctt ccttagttct
     2041 gcaaaatgaa gtggggatta ttcttcaatc gctaacaatt gcaatgaacc acggctaaaa
     2101 tccgccagct ttgcggaaaa ttcagccact ttcgccgccg gaagcgccac ccgcagcaga
     2161 acgaatgcct gataatcact gttgataatt ttgccgtcac actgccccag caacgcttca
     2221 atgccggtta actgactata ttcacattgc aaagtatatt cggttaatgg cgtcttgcgt
     2281 tgggtcgtta gctggcgcag cgcctgattc acgccgccgc catacgcttt cactaaccca
     2341 ccggtgccaa gcaatatgcc gccgtagtag cgcactacca cagcggtaat ttccccgatg
     2401 ccgctgccca ttagctgggc gagcatcggt ttacctgccg ttcccgccgg ctccccgtcg
     2461 tcagagaaac ccagctgttg agaatcatcc ggcgcacccg ccacccacgc cacgcaatgg
     2521 tggcgggcat cggggtgttc tgcccgcacc gattcaacaa acgctttcgc cgcctcaacg
     2581 ccatcggtat gcgccaacat cgttatgaag cggctctttt tgatctcttc aacgaccgtg
     2641 accggtgccg caggaattaa ccagctttcc atcacgccag tttcagatcc cgggtcatgt
     2701 tttccacgtt attttcgtgg atcaccacgt tgtcttcgat acgaatgccg ctgaacggtt
     2761 tcagtgcttc aattttctgc cagttgaagt gcttgctgaa ctgcccttca cgccacggtg
     2821 ccagtagcga ttcgatgaag tagatacccg gttcgatggt taacaccatg cccggctgga
     2881 gaatacgggt gcagcgcagg tacggatatt ttgccggtgc cgcgaggtgt gtaccgctat
     2941 catcctgcat aaaaccggct acgtcatgca cctgcaggcc cagcggatgg ccgataccgt
     3001 gcggcataaa cggtccggtg agatcgtttt cgaccatcgc ttcttcactc atatcggtga
     3061 tgatttgatg tttacgcagc aatttggcga tgcgctgatg gaactggagg tggtaatcca
     3121 catagctgac gccagctttc atggtagcga tcagcgcaag ttgttcatca tttacgtctt
     3181 tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca ggtacgagtc aggtcagccg
     3241 catagccgtt atattcggcc ccggcatcca gcaggaagct gcgcatctct tccggtgcct
     3301 gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc gttaagcgcc acaatgttgc
     3361 tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag ataggcgatg ttgatatcga
     3421 actcgctcat gccagaacgg aacgcttctt ctgccgcacg atgaccgttg accgccattt
     3481 tctgcgcttc acgcatacag gccagttcgt actcggtttt gaaggagcgg tagtaatgca
     3541 ggtagtcgat cacccctttc gggttgatgt tgctggcctc aatacccagt tgcagcgcac
     3601 gttccggcac cggaccgata taaccgatat tgccgcgcgc agcgggcagc aggctaccaa
     3661 tgccatcggc tttcggcagt gcgatcactt ccacatcttc agtccagaag gaggtcggca
     3721 gcggttcgac gttgtgccag taatcaaccg gcagatagaa ccacagtttc ggcttgttca
     3781 cgccatccac cagcaaccag cagtttggca cctgagttac cggcacccac gctttgaatt
     3841 gcgggttcac tttaaacgga tagggatgat cgtcgagaaa aacgttgaac agctcgccgg
     3901 agtgaataag taatgcatcc agcttgaagc gcgccagcgc atcgcgagtc cgttcttgta
     3961 aggtagctat atgattttta tagagcgagg ccagtgattc cattttttac ccttctgttt
     4021 ttttgacctt aagtctccgc atcttagcac atcgttcatc cagagcgtga tttctgccga
     4081 gcgtgatcag atcggcattt ctttaatctt ttatttacat gtttttaaca caaaatacac
     4141 acttcgatgc atctggtacg accagatcac cttgcggatt caggagactg acatgcttta
     4201 caaaggcgac accctgtacc ttgactggct ggaagatggc attgccgaac tggtatttga
     4261 tgccccaggt tcagttaata aactcgacac tgcgaccgtc gccagcctcg gcgaggccat
     4321 cggcgtgctg gaacagcaat cagatctaaa agggctgctg ctgcgttcga acaaagcagc
     4381 ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg ttcctcgttc ctgaagaaca
     4441 gttaagtcag tggctgcact ttgccaatag cgtgtttaat cgcctggaag atctgccggt
     4501 gccgaccatt gctgccgtca acggctatgc gctgggtggt ggctgcgaat gcgtgctggc
     4561 gaccgattat cgtctggcga cgccggatct gcgcatcggt ctgccggaaa ccaaactggg
     4621 catcatgccg ggctttggcg gttctgtacg tatgccacgt atgctgggcg ctgacagtgc
     4681 gctggaaatc attgccgccg gtaaagatgt cggcgcggat caggcgctga aaatcggtct
     4741 ggtggatggc gtagtcaaag cagaaaaact ggttgaaggc gcaaaggcgg ttttacgcca
     4801 ggccattaac ggcgacctcg actggaaagc aaaacgtcag ccgaagctgg aaccactaaa
     4861 actgagcaag attgaagcca ccatgagctt cactatcgct aaagggatgg tcgcacaaac
     4921 ggcggggaaa cattatccgg cgcccatcac cgcagtaaaa accattgaag ctgcggcccg
     4981 ttttggtcgt gaagaagcat taaatctgga aaacaaaagc tttgtcccgc tggcgcacac
     5041 caacgaagcc cgcgcactgg tcggcatttt ccttaacgat caatatgtaa aaggcaaagc
     5101 gaagaaactc accaaagacg ttgaaacacc gaaacaggcc gctgtactgg gcgcaggcat
     5161 catgggtggc ggcattgctt accagtctgc gtggaaaggc gtaccggttg tcatgaaaga
     5221 tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc gcgaaactgc tgaacaagca
     5281 gcttgaacgc ggcaagatcg atggtctgaa actggctggc gtgatctcca cgatccaccc
     5341 aacgctcgac tacgccgggt ttgaccgcgt ggatgttgtg gtagaagcgg ttgtcgaaaa
     5401 cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa aaagtacgcc cggataccgt
     5461 actggcgtct aacacttcaa ccattcctat cagcgaactg gccaacgcgc tggaacgccc
     5521 ggaaaacttc tgcggaatgc acttttttaa cccggtacac cggatgccgt tggtagaaat
     5581 tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa gttgtcgcct gggcgagcaa
     5641 gatgggcaag acgccgattg tggttaacga ctgccccggc ttctttgtta accgcgtgct
     5701 gttcccgtat ttcgccggtt tcagccagct gctgcgcgac ggcgcggatt tccgcaagat
     5761 cgacaaagtg atggaaaaac agtttggctg gcctatgggt ccggcatatc tgctggatgt
     5821 cgtgggcatt gataccgcgc atcacgctca ggctgtcatg gcagcaggct tcccgcagcg
     5881 gatgcagaaa gattaccgcg atgccatcga cgcgctgttt gatgccaacc gctttggtca
     5941 gaagaacggc ctcggtttct ggcgttataa agaagacagc aaaggtaagc cgaagaaaga
     6001 agaagacgcc gccgttgaag acctgctggc agaagtgagc cagccgaagc gcgatttcag
     6061 cgaagaagag attatcgccc gcatgatgat cccgatggtc aacgaagtgg tccgctgtct
     6121 ggaagagggc attatcgcca ctccggcgga agcggatatg gcgctggtct acggcctggg
     6181 cttccctccg ttccacggcg gcgcgttccg ctggctggac accctcggta gcgcaaaata
     6241 cctcgatatg gcacagcaat atcagcacct cggcccgctg tatgaagtgc cggaaggtct
     6301 gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg gttgagccag cccgtccggt
     6361 tggcgacctg aaaacggctt aaggagtcac aatggaacag gttgtcattg tcgatgcaat
     6421 tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt aacgtgcgtg cagaagatct
     6481 ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg gcgctggaag cggcggccct
     6541 cgacgatatt tactggggtt gtgtgcagca gacgctggag cagggtttta atatcgcccg
     6601 taacgcggcg ctgctagcag aagtgccgca ctctgtcccg gcggttaccg tcaatcgctt
     6661 gtgtggttca tccatgcagg cactgcatga cgcagcacga atgatcatga ctggcgatgc
     6721 gcaggcatgt ctggttggcg gcgtggagca tatgggccat gtgccaatga gtcacggcgt
     6781 cgattttcac cccggcctga gccgcaatgt cgccaaagcg gcgggcatga tgggcttaac
     6841 ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa atgcaggatg cctttgccgc
     6901 gcggtcacac gcccgcgcct gggccgccac gcagtcgggc gcatttaaaa atgaaatcat
     6961 cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag tttaattacg acgaggtgat
     7021 tcgaccggaa accaccgtgg aagccctcgc cacgctgcgt ccggcgtttg atccagtaaa
     7081 cggtacggta acggcgggca catcttctgc actttccgat ggcgcagctg ccatgctggt
     7141 gatgagtgaa agccgcgccc atgaattagg tcttaagccg cgcgctcgtg tgcgttcgat
     7201 ggcggtcgtt ggttgtaacc catcgattat gggttacggc ccggttccgg cctcgaagct
     7261 ggcgctgaaa aaagcggggc tttctgccag cgatatcggc gtgtttgaga tgaacgaagc
     7321 ctttgccgcg cagatcctgc cgtgcattaa agatctcggg ctaatggagc agattgacga
     7381 gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat ccgctgggct gttccggtgc
     7441 gcgtatcagc accacgctgc tgaatctgat ggaacgcaaa gacgttcagt ttggtctggc
     7501 gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg tttgagcggg tttaaccact
     7561 tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc ctgcaccaat gtaggcctga
     7621 taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg acatagatct
     7681 aaatgccact gaataaagtt ttagttgccg ttcttcccgc ctgtcagggg cgggtttttt
     7741 tatatctcag ataaatgcaa acgcatcgcc aaacagacga tcttcccgcg cattacgctc
     7801 actgcaaaac aggtcacgag caattttcgc catctcaaaa cgtccggcaa tatagatatc
     7861 atgctctgcc agcgtaccat gatcctgcaa gaccgccgtt aacacggtgc cagtacgccc
     7921 acgccagccc gcttccggtt gttcaaccac cggcaccaca cgcaggccag gatgcttcag
     7981 cgaaagcgcc tcaagctcgg agagatcata aagatgttgc tcctcacgcc caccccagta
     8041 aatggtgata tcgcgattgg gattacgcgc caacgccgtc aataaaatcg agcgcgcata
     8101 ggagaacccg gtgccgcccg caatcaaaat catcggacgc tcttcatcat cgcgcagcca
     8161 cgcttctccg tagggaatat cgaccacgat ttgataatct ttgaggatgc ggtccatgac
     8221 tgctttcgcg taaaggttga tttcagaagc gccaatatgc agctctataa accctttttc
     8281 atccggcgtc gaagccattg aaaacggacg tttgtcgcgc tcatccatca ctaccatcaa
     8341 atactgacca gcacgaaaag aaaaggccgc gtctggcacg atgcggacac gatatacggt
     8401 atccgtgata gcttctaccg aggtcacttt acagcttaag gttgtcatgc gctttctctg
     8461 tcggatcgat aaatagggca aaacaaacgc gcatcaggcg cttttaccgt tgttaaaaat
     8521 agccagttca tcccagatgg cgtcaatatg cgcgacaaca tctggatctt ttttgatggg
     8581 acgtccccat tcacgctggg tttcccccgg ccatttattc gtggcatcca gccccatttt
     8641 tgaacccagc ccggagacag gcgaggcaaa atccagataa tcaataggcg tattttctac
     8701 cagaacagta tcccgcgccg ggtccatacg ggtggtaatc gcccaaatca catcgttcca
     8761 gtcgcgtgcg ttgacgtcat catcgcaaac gatcacaaat ttagtgtaca taaactggcg
     8821 taagaacgac cagacgccca tcatgacgcg cttcgcgtgt ccggcgtact gttttttgat
     8881 cgtcactacc gccagacgat aagaacagcc ttccggcggc aggtaaaaat cgacaatttc
     8941 cgggaactgt ttttgcagaa tcggtacaaa cacttcgttc aacgccacgc ccagtaccgc
     9001 gggctcatct ggcggacgcc cggtataggt ggaatggtaa atcgcatctt cacgctgggt
     9061 aatatgcgtc acggtaaata ccgggaaact atcgacttcg ttatagtaac cggtgtggtc
     9121 gccatacggc ccttccggcg cagtttcgcc ttgttcgata tacccttcca gcacaatctc
     9181 cgcactggcg ggcacttcaa ggtcattgga gatacacttc accacttcgg tcttggtacc
     9241 gcgcagcaat ccggcaaacg catactctga aagtgtatcc ggaacggggg tgactgcacc
     9301 gagaatcgtg gcgggatcgg cacccagcgc cacagaaacc gggaaacgtt cgcccggatg
     9361 cgccgcacac cactcctgat aatccagcgc gccgccgcga tgcgacagcc agcgcataat
     9421 cagtttgttt ttaccaatca gctgctggcg ataaatgccc agattctgcc gctctttatg
     9481 cgggccgcgc gtcactgtca gcccccaggt aatcagcggt gcggcatctt ccggccagca
     9541 ggtcataatg ggaatgcgat tgagatcgac gtcatcgcca gagacgattt tttgttggca
     9601 gggcacacca cgcagccgct ttgtcggcat gttcaatact tgcttaaact gcggcagttt
     9661 atcaaacagg tcgcggaaac cttttggcgg ctccggctct ttcagaaacg ccaataattt
     9721 accaacttca cgcagcgccg aaacatcttc ctgtcccatg cccatcgcca cgcgctttgg
     9781 cgtaccgaac aggttgcaca gcaccggcat tgagtagcct ttagggtttt cgaacaacag
     9841 cgcaggccca ccggcacgca aagtgcggtc agcaatttca gtgatttcca gatgcggatc
     9901 caccgggagc gtgatacgtt ttagctcacc ctgctgttca agcagcgtca agaagtcgcg
     9961 taaatcgtta tatttcatgg cgtccattgt agcctcttaa tctacgccca ttatacggcg
    10021 ttcatctttg cgatgctgta aatttgttaa attagcgtga actctgacgg tataacgcaa
    10081 accggggaat ataattaact tagcgtaaag cttttgctat ccttgcgccc cgattaaacg
    10141 gataagagtc attatgcaat cctggtattt actgtactgc aagcgcgggc aacttcaacg
    10201 tgcccaggaa cacctcgaaa gacaggctgt gaattgcctg gcaccgatga tcaccctgga
    10261 aaaaatcgtg cgtggaaaac gtactgcagt cagtgagcca ttgtttccca actacctgtt
    10321 tgtcgaattt gatccagaag tgattcatac cacgactatc aacgcgaccc gcggtgtcag
    10381 ccacttcgtg cgctttggcg cgtcgccagc gatagtccca tcggcggtga ttcatcagct
    10441 atcggtatat aaaccgaaag acattgtcga tccggcaaca ccttatccgg gtgataaggt
    10501 gattattacc gaaggcgcgt tcgaaggctt tcaggccatt ttcaccgaac ccgatggtga
    10561 ggctcgttcc atgctattgc ttaatcttat taataaagag attaagcaca gtgtgaagaa
    10621 caccgagttc cgcaaactct aaaacgcaat cccaaacagt gttttgacat tggcatccgt
    10681 ggtggcagcc agccatgcgg catcttctcc acgccagtgc gcaatacgtt gcaaaatatg
    10741 gtgcagatgg gctggctcgt tgcgccggga tgatggcttt ggcgtgaaat cgcgcgggag
    10801 cagatacggc gcatcagttt cgatcagcaa tttctccgcc ggaatcaacg gcaacaattc
    10861 ccgcagctcc agcccgcgtc gttcatcgca aacccaaccg gtaatgccga tataaattcc
    10921 acgcgccacg cacgcctgca tctcttcgcg tgtgccggta aagcaatgaa gaaccgcacc
    10981 aggcagttta tccagccacg gctccagcaa tgtcataaac cgctcgtggg catcgcgaca
    11041 gtgcataaat accggcatgt ttaattctgc ggcaatgcgt agctgagcaa caaaagcgcg
    11101 ttcctgctct tccggcgtcg aaaagttgcg gttaaagtcg agaccacatt caccaatcgc
    11161 caccacttct ggctgcgagg ccagctcaat aatcgcttct tcagtcgcag cttgccactg
    11221 gctgctgtcg tgaggatgta cgcccgccgt tgaccaacag gacgaatact gacgcgccag
    11281 cttttgcgcc tgctggcttt cacgcaggtt agtgccggtg atgagtagcc cattaactcc
    11341 cgcgtcaaaa gcgcgcgcta caacatcatc acggtctttc gcaaattgcg aactggtcaa
    11401 attaacgccg atatcaaaca tcctgtactc catatgacaa ccgccctgac gggcggttga
    11461 atttattctt cagttttttc gctttctgct tcagcgtcgt tttcctcttc ccgatttcgc
    11521 cctttaccaa cgtaaaagcg tgagaagaag acaccgattt caaacagaca gtacatcggg
    11581 atcgccaata gcgtttgcga gaagacatcc ggcggcgtca gcaacatccc gacaacgaat
    11641 gcaccaacca gcacatacgg gcgttttttg cgtaagtctt ctggcgaggt aatccccatc
    11701 cagcacagca gcacaattgc caccggcact tcaaaggaga caccaaacgc cataaacagc
    11761 gccataacga agcttaaata gctggcgatg tcggtggata cctgcacccc ttccggcgcg
    11821 gtattggcaa ggaagccaaa tgccagcgga aagaccacaa agtaggcgaa tgccatgccg
    11881 atataaaaca gcagagagct ggaaaccagc agcggcacca ccaggcgacg ttcatgctta
    11941 tacagcgctg gggcgataaa tgcccacacc tgatagagaa tcaccggcgc tgacagaatc
    12001 agcgacacca taaaggtcag cttgatcggc gtaaagaacg gcgaggccac gtcggtggcg
    12061 atcatcgttg aaccttgcgg caactgcttg atcaatggcg cggataccag gtgatagatg
    12121 tcattggcga aatagaccag acacaggaat atcacgatca ccgcgataat gcagttcagc
    12181 agacgcttac gcagctcaat tagatgcgtg ataagcggtt gagtatcttc tacagacatg
    12241 tttacggttt atcactcgac gaaggggaag gtgcagcggt tttcggttca gcgtccgcag
    12301 caggttttac caccggctct ggcgtggttt ctggcttctg ttccggcgaa ctggcctgcg
    12361 tttgtgcagc ggcaggcgtt acgccctcat gcgcagcttc attatctttc accaccgggt
    12421 tatggatggt gtgcgcttca tcgctcgcct tttctggatc gtttgcaacg taggaacgct
    12481 tcatcgactc cgcggcctgg cgtagttcat ccatcgacgc tttcagttcg ggcgtcaggt
    12541 tagtgaggct cgccttttca acctttttca gactgtcctg aaactcctgg agttttaact
    12601 cctggatcag ttcgttctgc accgttgtcg ccagtgaacg caacgcgcga atccagcccg
    12661 ctaccgtttt taccgccaca ggcagtcgtt gcggccccag aacgacgagg ccgatgatga
    12721 acaccaatag cagttcgcta aaaccgatat caaacacgga ttacacctgc tctttatcgt
    12781 ggcgcttcgc gtcttctgtt ttagcctgtt cctgattcgt atccgcctgc ttatcggcga
    12841 tagttttcgc agtaaaatca gcatcctgac tggttttatc ctgctttggt tcatcatcgc
    12901 tcattgcttt tttaaagcct ttgatcgacg caccaagatc ggaaccgatg gagccgagct
    12961 ttttggtgcc aaaaagcagt acaacgatga cggcaataat caataactgc caaatactga
    13021 taccacccat acatgttcct ctgtggtaga tgatgattaa acaaagccgc attatacgtt
    13081 acacggcccg ccttgagcga tgaaaaaatc agcgtgtttt gcgccaaccg acaaaccagg
    13141 cgatcagacc acctgccatt aaccagccgg gcatcagccc ccactcaggt cggctgacca
    13201 acaagaatgt gccacttaat actaacgtag cgccaattcc gagaaaataa cgcgattgtc
    13261 cctgacgtac atgatttgac tgaagctcgc gggcaatctt atcaacactg tgctgtaaat
    13321 acttgccctg gcgcaaactg tcgtaaacca attcaggcag ttctggcatt ttttcgaccc
    13381 agaacggcgc tttttcttta aatgctctca ccagcgcagg aataccgacc tgatctttaa
    13441 tccacgactc caggaaaggc ttcgctgttt tccataaatc gagctgcgga taaagctggc
    13501 gtcctacccc ttcgacatag agcagggttt tctggagtaa caccagttgc ggctgcactt
    13561 ccatattgaa gcgacgcgcc gtattaaaca gatttaacag tacatgtcca aacgaaattt
    13621 cggccagcgg tttctcaaag ataggttcac agaccgtacg aatggcaaat tcgaactctt
    13681 caacgttggt atctggtggc acccagcctg aatcgacgtg tagctctgcc actttgcgat
    13741 agtcgcgatt aaagaaggcg ataaagtttt ctgccagata gcgtttatct tctttgttta
    13801 gcgagccaac aatcccgcaa tcaatgccga tatatttcgg gttttccggg tgttcatagc
    13861 ttacgaagat gttgccaggg tgcatatcgg catggaaaaa gctgtcgcga aagacctgag
    13921 tgaagaacac ctgcacgccg cgttccgcca gcaatttcat gttagtgccg tttttctcca
    13981 gcgccgcaac atcagacacc ggaatgccgt aaatgcgctc catcaccatc atcccttcac
    14041 tacaatagtc agggtaaact tccgggatgt agagcatcgg gctgtcttca aaattgcgcc
    14101 gaagctgaat ggcgttggca gattcccgca gcaaattcag ttcatcaatc aaggtctttt
    14161 cgtactcgcg caccacttcg gttgggcgca gacggcgacc atccggcagc aaacgcggca
    14221 cccagcgagc cagacggtag ataagtttca gatccgcttt aatgaccggc aaaatatccg
    14281 ggcggatgac tttaatcacc acctctttac cattcgattt caatcgcgcg gtatgaacct
    14341 gggcgataga agcagaagcc agcggcttga tttcaaaatc gtcaaaccac gcttctaccg
    14401 gcaagccgcc cattgcagct tcaatctgct gcttcgccag cttgccatca aacggagcta
    14461 ctttgtcctg caataacgcc agctgatcgg caatatgcgg tggaaaaaga tcgcggcggg
    14521 ttgataacat ttgcccgaac ttgatccaaa ccggccccag ttcttgcagg gccagtcgta
    14581 gtcgctcacc taaaagtttg tctttatgcc gatttggcat ccagaataat gagtatcgcc
    14641 atagccgtag cggcagggtg atacgcattt tggggatcag ttcatcaagt ccgtagctta
    14701 aaaaagtgcg aatgatgaaa tataggcgcc gtacttcacc tggcgtcatt tagcctccag
    14761 tttttccagc cgtttggtca gggcatcaac agcacgctcg acggcagccg tctcttccgc
    14821 aaaccaggcc acttcaagcg gaccgggtgc catacgccac tcttcagtaa tggcttccgc
    14881 cacataacgt tgctggcgct taatgccgtg atgcaggaac tttgcgcctc cgcgcatggc
    14941 tttgctgatt ccttcagcgg cgatatcacc ggtataaggg gccagcagtt ccgcagggtc
    15001 gaactctgcc agatctgcca gcgcaacgaa gttttgcacc acctgaatat cgccctgcac
    15061 ttccagctca ccactgcgaa tcagtgcggt aagctgctgg cgatcgcgaa gtttcggcaa
    15121 cacactggcg taggcgataa cggtgcagtc agcatcgcct gcccattcgc ccagtacatc
    15181 aacctggcgt tcgctgaaca ccagaatcaa tgacgtcgaa aagcctttta cctccacgcg
    15241 caatacttta cccagcagac gcgagcgggc tgttttcagc gcgggtgagc gatacaggaa
    15301 ggtgttgagc agactttcaa ttcctgccgt cactaaaggt ttaaaaggca tttccggtct
    15361 cctgtcagaa cttataacca cgatgcagcg ccacaacccc tgccgtcaga ttgtagtagt
    15421 cgacactttc gaatccggca tcctgcatca tggctttcag ggtatcctga tcgggatgca
    15481 tacggatgga ttctgccaga taacggtagc tgtcggcgtc gttcgcgacc agtgagccaa
    15541 tacgcggcag cacatggaag gagtatgcat cataggcttt gctcagcggc tcgataattg
    15601 gcttcgagaa ctcaagcacc agcaggcggc cgccgggttt cagcacgcga tacattgaac
    15661 gcagtgcttt atctttgtcg gtgacgttac gcagaccaaa cgaaatggtg atgcaatcaa
    15721 aggtgttatc cgggaacggc agcgcctcag cgttcgcctg aacatactca acgttgccaa
    15781 tcacaccgat attacgcagc ttctcgcgtc ccattttgag catggattca ttgatatcag
    15841 caaggaccac tttgccagtt tctccgacca ggcgggagaa tttcgctgtc aggtcgccgg
    15901 tgccaccagc cagatccagc acggtctgcc cacggcgtac gccgctgcaa tcaatcgtga
    15961 atcgcttcca caaacgatga ataccaaatg acatcaaatc attcatgaca tcgtattttg
    16021 atgccacgga atggaaaacg tgggcgacca tatccgcttt ttgttccttc gcgacggtct
    16081 gaaaaccaaa gtgcgtcgtt tcttgtgact tatccaccat ctcaatgcct gctcatcaaa
    16141 aaattgttcc agaagtgtaa cagattgggc gtcgatgccc tagatttcta cccggcttaa
    16201 ctacccctaa tgggctagcg cgactgctga ttatattcat catcgcgttg ataagcttca
    16261 tcattcggct gctccggaac cgaccgaagt cgatactctt catcctggct caccgcctgt
    16321 tcagccaaat ccggattaat ctcgcgttta atttctactc ctaaaccgcg aaacgcttct
    16381 gcctgcgcca gcacatttcc gcgacctgaa gagagttttt tcattgcctg gcggtagtta
    16441 tcctgcgctt tgtcgagact ttgaccaatc gcggacatgt catcgatgaa caaacgcatc
    16501 ttgtcgtaca gcttgctggc acgatcggcg atttgctggg cgttgcggct ttgatgctca
    16561 taacgccaca ggttggcaat agtacgcaac gccaccagca gcgtagtcgg gctaaccagc
    16621 atgatattgt ttttcaacgc ttcggtgatc agctccggct ggcggtcaag cgccagtaaa
    16681 aaagcgggtt caacgggaat aaacatcagc acgtaatcca gagttcgcag ccccggcagc
    16741 tgttgataat ctttgcgtcc cagcaaacgg atatggttac gcaccgacgc gatatgttcc
    16801 tgtagcgcgc tttcgcgggt gtagtcgtct tcggcgttaa aatagcgttc ataggcgacc
    16861 agcgtcattt tggcgtcgat caccacatct tttccctgcg gcagccgcac gatgacatcc
    16921 ggctgcatcc gcgagcgggc gtcattttcg atgctgacct gggtttcata ttcataccct
    16981 tcacgcagac cggaagcctc cagcacccgc gtcaatacta cctcgcccca gttgccctgg
    17041 gttttattgt cgcctttcag cgcgcgcgtc aggttgatcg cttcctgggc catttgcgcg
    17101 ttgagttgct ggagattgcg aatttcgtgg gtcagggtat ggcgttcttg tgcttcttta
    17161 ccgaagctgt cctgaacctg acggcggaaa ccgtccagtt gttcacgtag cggcgacaac
    17221 aggctgttca gactctgacg gttttgctca tcaacccggc gattgctgtg ctcaaaaata
    17281 cggttggcga ggttttcaaa ctgctcactg aggcgctgct cgctgttaat catctggcga
    17341 attttatcgt cagcatgttg ctgtgcggct tccatccgcg tggttacttc acgcagatcg
    17401 gcctccagag aggtgttaat actttgcagg ctgcgcactt cgttattgag taactcgcac
    17461 tctgcacgcc agtgctcgct ttgggtaatt tgttgttttg ccgcgcttaa ctccgcgacc
    17521 atctcttcac gttcagctaa ttgctcggct ttttgctgcg catgttgata actggcaaac
    17581 agccagccaa ttgccacacc caccaacgca ataactgcgt aaaccatgat tgagaaatcc
    17641 acaacgcctc ctgacgcaac aacttacccg cacaaaatag aattgtgctg tacaaacgtc
    17701 cagttggaaa ggcatttcct gcgaggcgct acgcaaaaat acaaaaggcc gaacgcgtcg
    17761 gccttcagac aggagaagag aattacagca gacgacgcgc cgcttccacc acgattttca
    17821 ccgcttggct ttcagtttgt ttcatcgtct cagcattcgg gatctcttgc tgggtgcggt
    17881 taacgataac acccgctacc ataccggcac gcaggccctg acttgcacac atggtcagca
    17941 gggttgcaga ttccatttca tagttcatta cgcccatcgc ctgccactct tccatagaac
    18001 ctttaaagtg acgaactacg cgaccagagt aagtatcgta acgttcctgg cctgggtaga
    18061 aggtatcaga agaagctgtc acgccaacgt gagttgtcgc gccaatggat ttcgcagctt
    18121 caaccagcgc agtcgtacat tcgaaatcag cgacagccgg gaattccagc ggtgcgaagt
    18181 gcaggctcgc gccatccaga cggacagacg ccgtggtaac caggacatca cccacattaa
    18241 tatgcggctg aatagcgccc gttgtaccga tacgcaggaa ggtgcgaatg cccagctgtg
    18301 ccagctcttc aacagcaata gaggtagacg ggccgccgat accggtagag cagacgataa
    18361 caggtttacc atccagctct gcacgccagg tggtgaattc gcggtgagat gccagcttaa
    18421 ccggcttatc catcagcgcg gcgatctttt ccacacgatc cgggtcgcca gggacgatgg
    18481 caagcgtagc cccttgtaaa tcgtttttag tgaggccgag atgaaaaaca tcagacttgg
    18541 acatatacaa ctcctctgtg aatcggttta gtcagaagaa gcaaaaagac actttaccga
    18601 agggtttaac attttttcgt gatactcatc accatgacgc aaatgcgttg cataaattta
    18661 cccaccaaaa gtgatgcaaa tcacataaat cactcttcat tctgcaggac tgcataaaag
    18721 atgagccgat tcaggcaatg tgatttgttg catgctgact atagttaaca ttgcgcaaca
    18781 tttttataag ggtacggaga ataccatggc aacaacacaa caatctggat ttgcacctgc
    18841 tgcatcgcct ctcgcttcga ccatcgttca gaccccggac gacgcgattg tggcgggctt
    18901 cacctctatc ccttcacaag gggataacat gcctgcttac catgccagac caaagcaaag
    18961 cgatggccca ctgccagtgg tcattgtagt gcaggaaatt tttggcatgc atgaacatat
    19021 ccgcgacatt tgtcgccgtc tggcgctgga ggggtatctg gctatcgcac ctgaacttta
    19081 cttccgcgaa ggcgatccga atgattttgc cgatatcccc acgttgctta gcggtctggt
    19141 agcaaaagtg cctgactcgc aggtgctggc cgatctcgat catgtcgcca gttgggcttc
    19201 ccgcaacggc ggcgatgttc atcgtttaat gatcaccgga ttctgctggg gtggacgtat
    19261 cacctggctg tatgccgcgc ataatccaca gctaaaagcc gcagtggcgt ggtacggcaa
    19321 actgacgggc gacaagtcgc taaattcacc gaaacaacct gttgatatcg caaccgatct
    19381 taacgcgccg gttctcggct tatatggtgg tcaggataac agcattccgc aggagagcgt
    19441 tgaaaccatg cgccaggcgc tgcgggctgc taatgcgaaa gcagagatta tcgtgtaccc
    19501 ggacgccggg catgcattca acgctgatta tcgcccgagc tatcatgccg aatctgcgaa
    19561 agatggctgg cagcgtatgc tggaatggtt taagcagtat ggtgggaagc ggagttagtt
    19621 tcctcttagt ctatatttga gatggtgagt gaacgagtgc gttttgctgc catctctgtc
    19681 ccccaaaaaa taatcagtat gaattgattg gagattacga ctccgctatt tgaccttaag
    19741 aaagtcggca agtcatagtc ataaatgagt ctcttcaatg gaagacacct tagagattta
    19801 ccatctactc ccattcacaa acaaacatta attattatca acacattcac attaattata
    19861 aatcatctca gaccttcctg ataaaaagat attaaagtga aacattttgt ttttcacatt
    19921 ttagttcatc acttttaggc gtaaataaag gtaaaaaaaa caacaatact atttctcttt
    19981 cacaaaagaa ttaacaaaag gttttttaag gatgagtgat attatttact tgaccataac
    20041 cggagaacaa caaggatgta tttcctctct ttgtggaacg agcgcatcaa taggtaatcg
    20101 ctggcagatt ggccatgaag atgaaatctt cgcgttctcg ctctctaata gtattacgaa
    20161 tgcaggcaaa gggtcacaat tgcatggcct tagtttctgt aaattcttat taacgctatt
    20221 aacaataacg agcagttgtt catggcgttt gacttctatc ggatcaatag atttggtcga
    20281 tgggagaaat attacaacat tcaactccgc ggcgcgctct tatcagcaat caatcacctg
    20341 tttactgaga acaatctgga tacagaagcc atcactgtca gttatgaata tatactctcc
    20401 aggcatctga ttgctaatac tgaatttagt tatttggcat ttcccgataa ctacaaccgt
    20461 ctgtttatac cacggccaaa aactaaagct ggtaatggtt caaaacactt aatagtaaag
    20521 cggttggcag actgcttgct gctggcggca tatacaatgg gaatatcgaa ggattcaggg
    20581 aaacagcaga caagttgggg ggagatgcac ctgctggtta cgatcaattt atggataaca
    20641 aagggttact catcgctggt gcttcaatag ttgctggagc agttctgggg agaatgagac
    20701 tccctgattt ggaatcactt gagcatttcg gagccagagg agcagtatca ggaag
//
LOCUS       DAJTNN010000061        20698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTNN010000061 DAJTNN010000000
VERSION     DAJTNN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.061"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..224)
                     /gene="hyfA"
                     /locus_tag="OO150_001971"
     CDS             complement(<1..224)
                     /gene="hyfA"
                     /locus_tag="OO150_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5516134.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDA"
     gene            complement(477..947)
                     /gene="bcp"
                     /locus_tag="OO150_001972"
     CDS             complement(477..947)
                     /gene="bcp"
                     /locus_tag="OO150_001972"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5516135.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(947..1519)
                     /locus_tag="OO150_001973"
     CDS             complement(947..1519)
                     /locus_tag="OO150_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5516136.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1665..2543
                     /gene="dapA"
                     /locus_tag="OO150_001974"
     CDS             1665..2543
                     /gene="dapA"
                     /locus_tag="OO150_001974"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5516137.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            2560..3594
                     /gene="bamC"
                     /locus_tag="OO150_001975"
     CDS             2560..3594
                     /gene="bamC"
                     /locus_tag="OO150_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5516138.1"
                     /translation="MAYSVQKSRQAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3807..4520
                     /gene="purC"
                     /locus_tag="OO150_001976"
     CDS             3807..4520
                     /gene="purC"
                     /locus_tag="OO150_001976"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5516139.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKRFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4688..5551
                     /locus_tag="OO150_001977"
     CDS             4688..5551
                     /locus_tag="OO150_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5516140.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5566..7581
                     /gene="tmcA"
                     /locus_tag="OO150_001978"
     CDS             5566..7581
                     /gene="tmcA"
                     /locus_tag="OO150_001978"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5516141.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            7655..8353
                     /gene="ypfH"
                     /locus_tag="OO150_001979"
     CDS             7655..8353
                     /gene="ypfH"
                     /locus_tag="OO150_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5516142.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFLGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(8463..8663)
                     /locus_tag="OO150_001980"
     CDS             complement(8463..8663)
                     /locus_tag="OO150_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5516143.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(8691..9818)
                     /gene="dapE"
                     /locus_tag="OO150_001981"
     CDS             complement(8691..9818)
                     /gene="dapE"
                     /locus_tag="OO150_001981"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5516144.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(9822..10178)
                     /locus_tag="OO150_001982"
     CDS             complement(9822..10178)
                     /locus_tag="OO150_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5516145.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            10559..10618
                     /gene="ypfM"
                     /locus_tag="OO150_001983"
     CDS             10559..10618
                     /gene="ypfM"
                     /locus_tag="OO150_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5516146.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(10717..13829)
                     /gene="acrD"
                     /locus_tag="OO150_001984"
                     /pseudo
     CDS             complement(10717..13829)
                     /gene="acrD"
                     /locus_tag="OO150_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(13992..15692)
                     /gene="narQ"
                     /locus_tag="OO150_001985"
     CDS             complement(13992..15692)
                     /gene="narQ"
                     /locus_tag="OO150_001985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR5516147.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQHLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            15898..17877
                     /gene="aegA"
                     /locus_tag="OO150_001986"
                     /pseudo
     CDS             15898..17877
                     /gene="aegA"
                     /locus_tag="OO150_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
     gene            17945..18520
                     /gene="nudK"
                     /locus_tag="OO150_001987"
     CDS             17945..18520
                     /gene="nudK"
                     /locus_tag="OO150_001987"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5516148.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            18646..19689
                     /locus_tag="OO150_001988"
     CDS             18646..19689
                     /locus_tag="OO150_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5516149.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(19799..20698)
                     /locus_tag="OO150_001989"
                     /pseudo
     CDS             complement(19799..20698)
                     /locus_tag="OO150_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
BASE COUNT         5002 a         5295 c         5539 g         4862 t
ORIGIN      
        1 atcgcatcat ccctctggga gatggcattg accgggcaga cctgcaggca aggggcttcc
       61 tcacagtgat gacacacgac aggggcagtg attgttgacg tcttcgccag ggccaggcgc
      121 gggtgttgct gtaaaccttg cgttttatgc acgtccgaac aggcagcgag acaggtatta
      181 catcctgtac accacagtgg ttcggccacc acaaagcggt tcatgtcggc tccttgaaaa
      241 tgatatgaaa cattgcattt catattgcat aaaggatgcc agtctgatta atttaatata
      301 ttcaataaat tatgtattaa cagggttctg aacggtacaa aaatgatggt cattaacgtt
      361 cagcaaaaaa tggattcgac acccgtgtcg caggggaaag cgagaatgtg atcttgatca
      421 cgagaagtga ggtgtgctga ccgcaagcgc agccagcacg gaatggagca aagtaatcag
      481 gcgtgttctt tcagccagtt cagcacaacg tcgtggtgat tgctggtttt aaaatcgtca
      541 aagacatgtt cgattttgcc atcagcgtca atcaggaagc tgatgcgatg aataccatcg
      601 taggttttgc ccatgaagga cttttcaccc cagacgccga attgttcgca cacctggtgg
      661 tcctcatcag acaggagcgt aaagttaagc agctcttttt ccgcaaaacg ggagagtttt
      721 tcgggtttat cggtgctgat acccagcaca tcaacgcccg cttttttcaa ctcatccatg
      781 ttatcgcgta agccgcaggc ctgtacggta cagccggggg tcatggcttt cgggtagaaa
      841 taaaccagaa cacgctgtcc ctggaagtcg gtcaaattaa cttgttctcc gtcttgatcc
      901 ggcaagctaa atttcggtgc gatatcaccg gctttcagtg gattcattac ttaactccat
      961 cctgttcatc atgttgggaa taattgacga cgttaatact gccttgtgca ttgagttctg
     1021 tacatagggc tttgaacgct tgctcaatat ttgctgcgtc cgcagatgcg gggctgtggg
     1081 cggtgatctg aatatgcaac tgcgcagccc gttcattttc agcaggttgc gtgcgcgaca
     1141 ccagctccgc aatgttcata tgatgcgcgt cgaaaagtgc tgtgaagcgt tcaattaaat
     1201 gcggggagtc tgcaacatcg acctgaaccc agacagatgc tggcattggc ggacgcggac
     1261 gcgccgtcgt gcgcttcatc acgattaaaa gatccagttc ggcacctttc aacggtaacg
     1321 ttgattcaat cagagtaatg gcattccatg aaccggaaag cagcatgata aacgtgaact
     1381 cttctcccag catcgccagg cgactgtctt caatattaca gccgcaacta ctgacatgac
     1441 gggtgatggt gttcacaatt ccagggcgat cggcacccaa cgcagtgatc accagataat
     1501 gttgcgatga cagtgtcaaa ctggttattc ctttaagggg tgagttgttc ttaaggaaag
     1561 cataaaaaaa acatgcatac aacaatcaga acggttctgt ctgcttgctt ttaatgccat
     1621 accaaacgta ccattgagac acttgtttgc acagaggatg gcccatgttc acgggaagta
     1681 ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt ctgtcgggct agcttgaaaa
     1741 aactgattga ttatcatgtc gccagcggta cttcggcgat cgtttctgtt ggcaccactg
     1801 gcgagtccgc taccttaaat catgacgaac atgctgatgt ggtgatgatg acgctggagc
     1861 tggctgacgg gcgcattccg gtgattgccg ggaccggtgc taacgctact gcggaagcca
     1921 ttagcctgac gcagcgcttc aatgacagcg gtatcgtcgg ctgcctgacg gtaacccctt
     1981 actacaatcg tccgtcgcaa gaaggtttgt atcagcattt caaagccatc gctgaacata
     2041 ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac tggctgcgat ctgctcccgg
     2101 aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg aatcaaagag gcaacaggga
     2161 acttaacgcg tgtaaaccag atcaaagagc tggtttcaga tgattttgtt ctgctgagcg
     2221 gcgatgatgc gagcgcgctg gacttcatgc aattaggcgg tcatggggtt atttctgtta
     2281 cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa actggcagca gaagggcatt
     2341 ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt acacaacaaa ctatttgtcg
     2401 aacccaatcc aatcccggtg aaatgggcat gtaaggaact gggtcttgtg gcgaccgata
     2461 cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg tgagacggtc agagcggcgc
     2521 ttaagcatgc cggtttgctg taaagtttag ggagatttga tggcttactc tgttcaaaag
     2581 tcgcgccagg caaaggttgc gggtgtttcg cttattttat tactcgctgc ctgtagttct
     2641 gactcacgct ataagcgtca ggtcagtggt gatgaagcct acctggaagc ggcaccgctt
     2701 gcggagcttc atgccccggc tggaatgatt ttgccggtga cctccggtga ttatgcaatc
     2761 ccggtgacca acggtagtgg tgctgtcggt aaggcgctgg acattcgtcc accagcccag
     2821 ccgctggcac tggtttctgg cgtgcgtacc cagttcacgg gcgataccgc ttcattgctg
     2881 gtggaaaatg gtcgtggcaa tactctgtgg ccgcaggtgg ttagcgtgct gcaggcgaaa
     2941 aactacacca tcacccaacg tgatgatgct ggtcagacac tgaccatcga ttgggtacaa
     3001 tggaaccgtc tggacgaaga cgagcagtat cgtggtcgtt atcaaatctc tgttaagccg
     3061 cagggttatc agcaggcggt tacggttaaa ctgctgaacc tggaacaggc gggcaaaccg
     3121 gttgcagacg cggcttccat gcagcgttac agcacggaga tgatgaacgt tatttccgcc
     3181 ggtctggata aatctgcctc tgacgccgcg aacgctgcgc aaaatcgtgc ctccaccact
     3241 atggacgtac aaagtgcagc tgatgacacc ggtttaccga tgctggtcgt acgcgggccg
     3301 ttcaatgtgg tttggcaacg tctgccagcg gcgctggaaa aagtgggcat gaaagtgacc
     3361 gacagcaccc gttcgcaggg taacatggcc gtaacttata agccgctgtc tgacagcgac
     3421 tggcaggaac tgggcgcgag cgatccaggc ctggcatccg gtgactataa actgcaggtt
     3481 ggcgatttag ataaccgcag cagcctacag ttcatcgatc cgaaaggtca tactctgact
     3541 cagagtcaga acgacgcgct ggtagctgtc ttccaggctg cgtttagcaa gtaaaaatac
     3601 agggctggag tcatccggcc cttttttctg atatgatacg caaacgtgtg cgtcggcaga
     3661 aaaatgcaat tttagcggta attcgcacga aatttgtttg tcggacgtag gtcggataag
     3721 gcgttcttgc cgcatccgac aaaacatccg gcacaccaga cagcaaaaga ttttaaaacg
     3781 ttaattcaca cccaggagtg ataaagatgc aaaagcaagc tgagttgtat cgtggtaaag
     3841 cgaaaaccgt atacagcacg gaaaacccgg acctgttggt gctcgaattc cgcaatgata
     3901 cgtcagcagg ggatggcgcg cgcattgagc agtttgatcg caaaggtatg gtgaacaaca
     3961 agttcaacta cttcattatg agcaaactgg ctgaagcggg tatcccgact caaatggagc
     4021 gtctgctctc cgataccgaa tgtctggtga aaaagctgga tatggtgccg gttgagtgtg
     4081 tcgtgcgtaa ccgtgctgct ggctctctgg tgaaacgtct tggaatcgaa gaaggtattg
     4141 agctgaaccc gccgctgttc gatctgttcc tgaaaaacga tgccatgcac gatccgatgg
     4201 tcaacgaatc ttactgcgaa acctttggct gggtgagcaa agagaacctg gcgcgtatga
     4261 aagagctgac ctacaaagcg aacgacgtgc tgaaaaaacg gttcgatgat gctggtctga
     4321 ttctggtcga cttcaagctg gagtttggtc tgtacaaagg tgaagtggta ctgggcgatg
     4381 agttctcccc ggacggcagc cgcctgtggg acaaagaaac actggagaaa atggacaaag
     4441 accgtttccg ccagagcctc ggtggcctga tcgaagccta tgaagccgtc gcccgccgcc
     4501 tgggtgtaca gctggactga tttttctgtt catcatcttg ccgtgcagct ggcacggcaa
     4561 gacaaccgct ctcggaatat atgcattgaa cgctcattcc tccgccattt catcccgctt
     4621 tcctgtggta atcttcctct gaaccaccta cgatcatttc tataatgaat atattgttga
     4681 ggtatctatg cgttggcaag ggcgacgtga aagtgacaat gttgaagaca ggcgcaacag
     4741 ctccggtggt ccgtctatgg gcggtcccgg ttttcgcctg ccaagcggta aaggcgggct
     4801 gattttactg atagtcgtgc tggttgcagg ctactatggt gttgatttaa ccgggttaat
     4861 gactggacag ccggtttccc aacaacaatc aacgcggtcc attagcccta atgaagacga
     4921 agctgcaaaa ttcacctcgg tgattctggc aaccacggaa gacacctggg gacaacagtt
     4981 cgagaagatg ggtaagacct atcagcaacc gaagctggtc atgtaccgtg gaatgacgcg
     5041 caccggctgc ggggcgggcc agtccattat ggggccgttc tattgcccgg cggatggcac
     5101 agtttatatc gatctctcct tctatgatga catgaaagac aaacttggcg cggatggcga
     5161 ttttgcccag gggtacgtta tcgcccatga agtcggtcat catgtgcaga aactgttagg
     5221 catcgagccg aaagttcgtc aactgcaaca aaacgcgacg caggcggaag tgaaccgctt
     5281 atctgtgcgt atggaactcc aggccgactg ttttgccggt gtctgggggc ataacatgca
     5341 gcagcaaggc gttctggaaa ccggcgatct ggaagaggcg ctgaatgcgg cgcaggccat
     5401 cggcgatgac cgtttacaac agcaaagtca ggggcgagta gtaccagaca gtttcacaca
     5461 tggcacttct cagcaacgct acagctggtt taaacgtggt ttcgacagcg gcgatccggc
     5521 acaatgcaat acttttggta aaagcattta atttccgggg cagggatggc tgaactgact
     5581 gcgctttaca cattaacagc gcaaatgaaa cgtgaaggga tccgccgctt gctggtgttg
     5641 agcggggaag agggttggtg ttttgatcat gcgcttaagt tgcgtgatgc cttacttggc
     5701 gactggctgt ggatttcgcc gcagccagat gctgaaaacc actgttctcc ctcggcgcta
     5761 caaactttac ttgggcgcga gttccggcat gcggtattcg acgcccgcca cggctttgat
     5821 gccgctgcct ttgcggcact tagcggaacg ttgaaagcgg gaagctggct ggttttgtta
     5881 ctccctgtat gggaagagtg ggaaaaccaa cctgatgccg actcgctgcg ctggagtgat
     5941 tgccctgacc ctattgcgac gccgcatttt gtccagcatc tcaaacgcgt acttacggcg
     6001 gataacgacg ctattctctg gcggcaaaac cagccgttct cgttggcgca ttttactccc
     6061 cgtactgact ggcaccccgc gaccggcgca ccacaaccag aacaacagca actcttacag
     6121 cagctactga ccatgccgcc gggcgtggca gcggtaacgg ctgcgcgtgg gcgcggtaag
     6181 tcggcgctgg cagggcaact catttctcgt attgcgggca gtgcgattgt caccgcgccc
     6241 gcaaaagcgg caacggatgt actggcacaa tttgcgggcg agaagtttcg ctttattgcg
     6301 ccggatgcct tgttagccag cgatgagcaa gccgactggc tggtggtcga tgaagccgca
     6361 gccatacctg cgccgttgtt gcatcaactg gtatcgcgtt ttcctcgaac gttgttaacc
     6421 actacggtgc agggctacga aggcaccgga cgtggttttt tgctgaaatt ttgcgctcgc
     6481 tttccgcatt tacaccgttt tgaactgcaa cagccgatcc gctgggcaca gggatgcccg
     6541 ctggaaaaaa tggttagtga ggcactggtt tttgacgatg aaaacttcac ccatacacca
     6601 caaggcaata tcgtcatttc cgcatttgaa caaacgttat ggcgaagcga gccagaaacg
     6661 ccgttaaagg tttatcagtt attgtctggt gcgcactacc ggacttcgcc gctggattta
     6721 cgccggatga tggatgcacc agggcaatat tttttacagg cggctggcga aaacgagatt
     6781 gccggagcgc tgtggctggt ggatgagggg ggattatctc aacaactcag tcaggcggta
     6841 tgggcaggtt ttcgtcgccc gcggggtaat ctggtggccc agtcgctggc ggcgcacggt
     6901 agcaatccac tggcagcgac attgcgtgga cggcgggtca gccggatagc agttcatccg
     6961 gctcgtcagc gcgaaggcgt tgggcaacag ctcattgcca gcgctttgca atatacgcaa
     7021 gacctcgact atctttcggt gagttttggt tacaccgggg agttatggcg tttctggcaa
     7081 cgctgcggtt ttgtgctggt acgaatgggt aatcatcgtg aagccagcag tggttgctat
     7141 acggcgatgg cgctgttacc gatgagtgat gcgggtaaac agctggctga acgtgagcat
     7201 taccgtttac gtcgcgatgc gcaagctctc gcgcagtgga atggcgaaac acttcctgtt
     7261 gatccactaa acgatatcgt gctttctgac gacgactggc ttgaactggc cggttttgct
     7321 tttgctcatc gtccgctatt aacgtcgtta ggttgcttat tgcgtctgct acaaaccagt
     7381 gaactggcat taccggcgct gcgtgggcgt ttacagaaaa atgccagtga tgcgcagtta
     7441 tgtaccacac ttaaacttgc aggccgcaag atgttactgg tccgtcagcg ggaagaggcc
     7501 gcgcatgcgc tgttcgcact taatgatgtt cgcactgagc gtctgcgcga tcgcataacg
     7561 caatggcaat tttttcactg actccttcaa ttttctccca tggtcattgt gagccagcgg
     7621 cgtaaaatta atgacatcag ttaaggagtt cgccatgaaa catgaccatt ttgttgttca
     7681 aagcccggat aaacctgtgc aacagttgtt actgcttttc ctcggtgtcg gggataactc
     7741 ggtggcaatg ggggaaatag gcagttggtt tgcaccgctg tttcctgatg cgctggtggt
     7801 gagtgtcggt ggcgcggagc cgagcggtaa tccggcgggg cgtcagtggt tttcggtgca
     7861 gggtattacg gaagataatc gccaggcgcg tgtggatgcc atcatgccga cgtttattga
     7921 aacggtacgc tactggcaga aacagagtgg agtaggggct aacgccacgg cgttaatcgg
     7981 tttctctcaa ggcgcgatta tggcgctgga gagtattaaa gccgaaccgg gccttgcttc
     8041 acgcgtcatt gcttttaacg gacgctatgc gagcctgccg gaaaccgcgt ctacagcaac
     8101 gacaattcac ctgattcatg gcggtgaaga tccggttatc gatctggcgc atgcggttgc
     8161 ggcgcaggaa gcgttaatca gtgccggtgg tgacgtgacg ctggatatcg tggaggattt
     8221 aggccatgcg atcgacaatc gcagtatgca atttgccctg gatcacttgc gttataccat
     8281 tccgaaacat tatttcgacg aagcgctaag tggcggtaag cctggcgatg atgatgtgat
     8341 tgaaatgatg taaaagacag attacctggc ctgataagac gcgtgagcgt cgcatcaggc
     8401 atcgttcacc attgccggat gcggcgttaa cgccttatcc ggcctacggt tgagcactca
     8461 gcttacttct tcggttgatc ctttttcggc cagtcgtcgt catcgtccca cttatcgttg
     8521 aaatcacgat gcgggggaag ctccggcttg ttggcgagaa atttcttgtg gtcgacgcgc
     8581 ttgagatctt taatcacgtt cagcaggacg cctaccaaaa agacaatcac cagaatccac
     8641 caatatttag ccagccagtc catgcttatt tcctcttgca gaaccactca tcaggcgacg
     8701 agctgttcca tgatacgttg atacatacgg gcaagtagct gcaggtcggc agcgttcaca
     8761 cattcattaa ttttatgaat agtggcattg accggcccga gttccaccac ctgcgccccc
     8821 atgcgagcaa taaagcgccc gtcggacgtt ccgcctgtgg tcagtagctg cggtttaatt
     8881 tcattatagt gctcaaccgc gttaacgacc gcatccacca gtttaccgcg cgcggtcaaa
     8941 aatggctgcc cggaaagcca ccaatccacc gtatagcgca gttgatgttt ttcaagcagg
     9001 gcaagcacct gcgctttgat catctcatca gtcagttcgg tgctgaagcg gaagttaaac
     9061 tgcacaaaca gttcacccgg aataacattg ttactgcccg ttcccgcctg aatattagca
     9121 atctgcatac tggtcgccgg gaaaaattca ttgccctgat cccactcaat agccactaat
     9181 tcattaagga aaggtgctgc gcgatgtact ggattgtcag ccagatgcgg gtaggccaca
     9241 tgcccctgaa cgccatgaat ggtaaggttg caggttaacg atccgcgacg accatttttc
     9301 accacatcac caaccacttc gatactcgac ggttcgccga ccaggcagta atcgagacgc
     9361 tcattacgtg ccatcaacgc ttcgacgact tttaccgtac cgttgtgggc actggcttct
     9421 tcatcagagg tgatcagaaa tgccagtcgc cccgtatggt tgggatgttg tgcgacaaag
     9481 cgttctgccg ctaccaccat cgccgccagc gagcctttca tatctgccgc accgcgcccg
     9541 aataacatgc catcacgaat ggtgggttca aacggcggat tgatccaacg atcggcgtcg
     9601 ccaggcggca ccacgtcggt atgcccggca aaggctaacg tttcaccttg cccacgccat
     9661 gcccaaaaat tctgcgtatc ggcaaagtcc atgcgttcaa cggtaaaacc gatcgcctgc
     9721 aaacgttcaa tcaacaaagc ctggcatcct gcgtcatcag gactcaggga agggcggcga
     9781 ataagctgtt gtgtcagctc aataaccggg cacgacatag actacacctc atggaaaaat
     9841 tgctgataac tggaatcact gaaacccagc agcataggct taccaggcgc gcagagcaat
     9901 ggacgtttga taattgaagg catttcagtc attaatgccg ccgcagaggc cgcatcggtg
     9961 attttattgc gggtggtttc gtccagttta cgccaggttg taccacgggt gttcagtaac
    10021 gcttcccagc ctaattcgtt gataaaaccg ttcaataatt cgctgtccag cccatcgacg
    10081 cggtaatcat gaaaacgata gtcgatgtta ttggcttcca gccaacggcg agccttttta
    10141 atggtgtcac aatttttgat gccgtaaagt gtaaccataa tgaatccttt ttcgatctat
    10201 gagaataaat actcatttaa gggtgaaaac attttgcctg caaatgatta tcaggaaata
    10261 taacattatc gaagtattca aattaacaac tattaaataa tattaaacat ttttaacgta
    10321 aacattaaga atattcatct tgttatctgt ctgtatttta accagaatta aatcagtatt
    10381 agatgccgta tgaatatctc gaaatgttgc tgtgtatcac atacaaatta tcaccgcagg
    10441 accatagtat ttacataagg tttgtccccc ggaggtagtc atcgcagcaa tcgagtaaat
    10501 tagcttcacc acggtatatt gtttccgtag aatacccata attataagag aggttgttat
    10561 gattgaacgt gaactgggga actggaaaga ctttatcgaa gttatgcttc gtaagtaatt
    10621 cctgagacaa agtacaaaca gcaagaaccc gctgagcagg ttcttaatcg gaaagttgaa
    10681 aaaggcgaca cattagcatg tcgccttttt tattgcttat tccgggcgcg gcttcagcgg
    10741 gaagcggcgg cgcaccagta caaagaacag cggcacgaag taaatagcca gaatagtggc
    10801 cgaaatcatc ccgcccatta cgccagtacc caccgcatgc tgaccaccgg aaccggcacc
    10861 cgtgctggtt gccattggca atacgccgaa gataaatgcc agcgaggtca tcagaatcgg
    10921 gcgtaaacgc tgacggcagg cgtggagcgt cgcttcaaac aggtcgtggc ctttttggtt
    10981 catctcgtta gcaaactcga cgatcaggat ggcgtttttc gccgataaac caatgaccgt
    11041 taacaggccc acctggaagt aaacgtcgtt ttccagcccg cgcatccagg ttgccagcag
    11101 cgcgccgata acccccagcg gcacgaccag cattaccgag aacggcaccg accagctttc
    11161 atacaacgcc gccagacaca ggaataccac cagcaaggaa atggcgtaca gcgccggagc
    11221 ctgcgcgccg gaaagccgct cctgatacga catcgccgtc cactccagac caaagccgtt
    11281 tggcaactgc ttcactaacg attccattat atccatcgcc gtaccggtac tgacccccgg
    11341 tgcggcttcc ccaacaatct caaccgcaga ataaccgtta tagcgttcca gacgcggcga
    11401 gccagtctcc cagcgtgagg tcgcgaaagc agagaagggc accatgccgc catctttatt
    11461 tcggacatac cagagattaa tgtcatctgg cagcatgcga tacggcgcag ctgcctgcac
    11521 atagactttc ttcacacgac cgcgatccat aaagtcattc acatagctcg aaccccaggc
    11581 ggtttgcagt gtgtcgttaa tatcgtcgat agcaacgccc agcgcctgag ctttacgctg
    11641 gtcgatatca atctgcaact gcggactgtc atcgaggccg ttatggcgca cacgggttag
    11701 ctccgggttt tccgccgcca gcgccagcaa ctggttacgt gctgccatca gcgcatcgtg
    11761 acctgctcca gcgtggtcct gcaactccat atcaaaaccc gcagaactac caagaccgct
    11821 aattgctggc gggctgctgg cgataacgcg agcttcttta atcttgttaa acgcctttgt
    11881 tgcacgctcg ataatggcaa acgaggtgcc ggtcttactg tcgcgttcgc tccagtcttt
    11941 caggcggata aacattcgcg ccacgttttg cccgttaccc ccagggccag aaccaacggt
    12001 ggcaaacacc gacatgatgt tgtctttttc atgggtgaag taatatttct cgatttgctc
    12061 aacgactttc agggtctgtt gttgggttga accgctgggc aattgtaccg aggtagtaaa
    12121 catgccacgg tcttccagcg gtaagaacga cgtcggcaaa cgcaggaaca ggaacaccat
    12181 gccgccaagc agcaggacat aaatcacaat ccagcgcagg ctgcggtgga gaattttcgc
    12241 cacccctttt tcgtagcgtt cggcgttgcg gttaaacatc tggttaaacc aggcaaaaaa
    12301 gcctttttgc ccgtgatgct cacctttttt cagtggctta agtagtgtgg cacacagagc
    12361 cggagtgagg atcatcgcta ccagtactga cagcaccatc gccgcaacaa tggtaataga
    12421 gaactggcga tagatggcac cggtggtgcc gccgaagaag gccattggta caaataccgc
    12481 cgaaagcacc atcgcaatcc cgaccagtgc cccctggatc tgccccatcg atttacgtgt
    12541 ggcttcgcga ggagcgagtc cttcctcact cataatacgt tcgacgtttt ccaccaccac
    12601 gattgcgtca tccaccagca gaccgatcgc cagtaccatc gcgaacatgg ttaaggtgtt
    12661 gacgctgtaa ccgaaggcgt aaagtacgga gaaggttccc atcaacacca ccggcacggc
    12721 gatagtgggt atcagcgtgg cgcggaagtt ttgcaggaac agatacataa cgaggaaaac
    12781 cagggcgata gcttccagca gcgttttcac cacgtcttca atggaggctt taacaaacga
    12841 ggttgtttca tacgccacct tgtattccag tccgtgcggg aagtactgcg ccagctcgtc
    12901 gagacgattg agcaccagct ccctgtcgcc atttcgtttg cgccggaggc cagttttacc
    12961 cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt ctccgccccc
    13021 atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcttgatt cacccgcaag
    13081 gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat ggtggcgttg
    13141 agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat ctgcgcgttc
    13201 tgtgactcaa tggcatcagt gacatcttta gccgtcatct ggaaactgtt gagtttcgcc
    13261 ggggccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc cccgacgcca
    13321 ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat atcctgttta
    13381 tccatcgaac catcggtaga gacgaaagca atggtcagaa tattggtatc gccggtttta
    13441 cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc tgattgcagc
    13501 tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa acttaaagtg
    13561 acagatgcct gaccggtgcc actgctctga gatgacatat acatgagatt atcgaggccg
    13621 gtcatatttt gctcgataac ctggggcacg gtgttttcca gcgtctgggc cgatgcgccg
    13681 ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg ttcaacgggc
    13741 aatgaaaaaa tcgccagggt ccctgtcaga cacaacagga ttgccagcac ccaggcaaaa
    13801 atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc tgcgtatcgt
    13861 tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg agaaaaaagg
    13921 agttaatgta aattatcatg cccttttata ctgaacctta agtgcaagta ttctttggtc
    13981 agtaggaggc attacatcaa ctgactttcc tcaccctctg cagagcgaaa gctaatactc
    14041 actaacgtgc cgccgccgga aggttgtgaa aaagtcagcg tcccacccag ccgttccgcg
    14101 cgttcgcgca tgatattcag accataatga ccttcgggtt ctttcggttc accgatacca
    14161 atcccgttat cacggatata aaccgtgtga ttgccgtccg gcgcggtgac gcaactgacg
    14221 gcgatttcgc tggcgttggc gtgcttcatc gcattcagca ccgcttcgcg aataatttgc
    14281 aacaaatgca cctgcatttg cgcatccagc gcaagggttg gcagacggca gtcgagggtc
    14341 agtttggcgc tggtttgatt ttgtaacgta tccagcattt ccctcaacgc ggaggggaga
    14401 tccgcctgct gcagcgtcag gcggaaagta gtcaacagct cgcgtaactg ccgataagca
    14461 tcattcaacg cctgggaaaa atcggccatg atactttgtg cggtggcgtt atcttccggt
    14521 atcgaacgct tcagtaacgt caactggata cgtaagtaag aaagtacctg agccagcgag
    14581 tcgtgcaatt cgcgggcgat ggttgcacgt tcttccatca acaataaatg ctgaaaatgc
    14641 ttctgcgcct gattaaagta caaaccgcgt cccagcatcg acgaaacgct gttaagcagc
    14701 ggttctgatg atgaaacgtg actattttgc cagtgcagtt cgccgtaaac cgtctcttgc
    14761 attgtcaccg gtaaaatctg catcggcaat tccgggtttg gttgcccttc gctaatccgc
    14821 cagttttcac cgacatttaa ctccagatat tcagccgctt cattgtcgcg aacaatctgc
    14881 aaaatatggc ggaaacaatg cacatcaatc tggcttgtgt tcagcgcctg cgaacattga
    14941 tacaacacct ccagacgacg cttggcctcg tggagatcgc gggtcttttc ttctactgac
    15001 gcctccagcg aaaggtacaa tttatgcagc tcgctcgaca tctggttaaa ggtttttgca
    15061 agcagaccaa gctcattcgg cagactggta tccagcggcg gcgagtcgaa ctgcccgtgt
    15121 tcaatacgtt gactggcggt aaccagctga ttcagcgggg caaccacctg atggcgtata
    15181 cggcgcaaag taaaaaagac cagcgtgaaa ataccgatac cgccagccag ggaaatcgcc
    15241 accaccagca gcattttgcg ttcagcgtag tgctgtaaag ccagcacgaa caggtctatc
    15301 tgattaacat aattattaat attggcctga taccacggca aatcgccctt gctgagccga
    15361 ttattcatct ccagccagtt ggcattcaga tgcgcatagc gagtttttac tgcttctggc
    15421 acataccaga cgttgagatt ggttaatacc ggtgaatgca gtgcctgctg aaacaactgg
    15481 cgatgtgcat tgagttgtgg actgccactt tgcaagtcgt agcccaggcg gtaactctgc
    15541 atacgcagcg atccggcaat attgatagcc tcagcgtcgc gcaaactgct cgccagagtt
    15601 agcagagcga tacccgtgga aagaatcgac agcagcacaa tgtaaaaaaa ggcccgggcc
    15661 agactggccg agacgggtcg tttaacaatc acacgcgtct tctccacaaa aaatcattaa
    15721 tgttccagtt cgctgaaacg caatttagtc agtgatggga ttatttgagc tgccacagtt
    15781 tatagcgaaa tagttactta ttctgggcac atgaacattg ccgattcccc tgtaagtcgg
    15841 gtaataacaa cactcatata aagaataagg tttttacaac caaaaaagaa ggtcgttatg
    15901 aatcgtttta ttatggccaa cagtcagcaa tgtctgggtt gtcatgcttg tgaaatcgcc
    15961 tgtgtcatgg cttacaatga tgagcaacat gtcctgagcc aacaccattt tcatccccga
    16021 attacggtta tcaaacatca gcagcaacgt agtgcagtga cctgtcacca ttgtgaagat
    16081 gcgccctgcg cccgtagctg ccctaatggc gcaatcagcc acgttgatga cagcattcag
    16141 gtcaatcagc aaaagtgtat tggctgtaaa tcctgcgtgg tggcctgtcc gtttggtacg
    16201 atgcaaatcg tcctgacacc cgtcgcggca ggaaaagtaa aagccacggc gcataaatgc
    16261 gacctttgtg cagggcgcga aaacggtcct gcctgtgtcg agaattgccc ggcggacgcg
    16321 ctgcaactgg tcactgacgt cgcactctcc ggcatggcga aatcccgccg cttgcgcacc
    16381 gcacgtcagg agcatcaacc gtggcatgcc agtaccgctg cacaagaaat gccggtaatg
    16441 agtaaagtcg aacaaatgca ggcaacgccc gcgcgtggcg agccggataa actggcgatt
    16501 gaagcgcgca aaaccggttt tgatgaaatt tatctgccat ttcgcgccga ccaggcacaa
    16561 cgggaagcct cgcgctgcct taagtgcggc gagcacagcg tttgtgaatg gacctgcccg
    16621 ctgcataacc atattccgca gtggattgaa ctggtgaaag ccggaaacat cgacgccgcc
    16681 gtcgagcttt ctcaccagac caacaccctg ccggaaatta ccggacgcgt ttgtccgcaa
    16741 gaccgtttgt gtgaaggtgc ctgtactatt cgcgatgagc acggcgcggt aactatcggc
    16801 aacattgaac gctatatttc agatcaggcg ttggcgaaag gttggcgtcc tgacttaagt
    16861 catgtcacca aagtggacaa gcgggtggcg attatcgggg caggtccggc agggctggcc
    16921 tgtgcggatg ttctgacccg caatggcgtg gcggtgacgg tgtacgatcg ccatccagaa
    16981 atcggtggct tgctcacttt cggcattcct tctttcaaac tggataaatc cctgctggca
    17041 cgccgtcggg aaatcttcag cgcgatgggg attcacttcg aactcaattg tgaagtgggt
    17101 aaagatgtct ctttggattc gcttttggaa caatacgacg cggtcttcgt tggcgtaggc
    17161 acttaccgtt ccatgaaagc gggtttaccc aatgaagatg cgccgggcgt ttatgacgcg
    17221 ctgccgttcc tcatcgccaa cactaaacag gtgatggggc tcgaagagct accggaagag
    17281 ccgtttatca ataccgccgg acttaacgtc gtggtactgg gcggcggcga caccgcgatg
    17341 gactgtgtgc gtactgcgct gcgccacggc gcgagtaacg tcacctgcgc ttatcgtcgt
    17401 gatgaagcta acatgccagg ctcgaagaaa gaggtgaaga acgcccgcga agagggggcc
    17461 aacttcgaat ttaacgtcca gccggtggcg cttgagctga atgaacaagg tcacgtttgc
    17521 gggattcgtt tcctgcgcac gcgtcttggc gagccggatg cctaggggcg tcggcgtcca
    17581 gtaccggtgg aaggcagtga atttgtcatg ccagccgacg cggtgattat ggcgtttggc
    17641 ttcaatccgc acgggatgcc gtggctggag tcgcacggtg taacggtaga caaatggggc
    17701 cgcatcatcg cggatgtgga aagccagtac cgttaccaga ccaccaatcc gaaaatcttc
    17761 gccggtggcg acgccgtgcg tggtgcggat ctggtggtta ccgcaatggc agaaggacgt
    17821 catgcggcac aggggattat tgactggctg ggggtaaaat cagtcaaatc tcactgatag
    17881 cctgcgcaga caaacccgac ttcacagcgt aagataattg ttcatttcgc gctgtggagt
    17941 cggtatgacg caacaaatca ccctcattaa agacaaaatt ctctccgata actatttcac
    18001 cctgcacaac attacctacg atctcacgcg caaagatggc gaagttatcc gccataaacg
    18061 tgaagtttac gatcgcggta atggtgcgac gatcctcctg tacaacgcga agaaaaagac
    18121 cgtggttctg attcgtcagt tccgtgtcgc tacctgggtt aatggcaatg aaagcgggca
    18181 gctgattgaa acctgcgccg ggctgctgga taacgacgaa ccggaagtgt gcattcgtaa
    18241 agaagcgatt gaagagacgg gctatgaagt gggcgaagtg cgcaaattat ttgaactgta
    18301 tatgtcgcca ggcggtgtga ctgagctaat ccactttttt atcgccgaat acagtgacaa
    18361 tcagcgcgct aacgccggtg gcggtgtcga agatgaagat attgaagtgc tcgagctgcc
    18421 gttcagccag gcgctggaga tgatcaaaac cggcgagata cgtgacggta agacggtgtt
    18481 attgcttaac tatttgcaaa cgtcacattt aatggactga aaaataacaa taatatttcg
    18541 ttgtttatta ttggctcaat ccgacaaatc cgattgagcc gcgctacctg cgcaacgaag
    18601 atacgacttg tgctgtttgt ttgaacttct ggggtcgtac cgtccatgcg ctatcgcatt
    18661 ttccttctct tttttttcgc tttgttgccg acgtctttgg tgtgggcggc accagcgcaa
    18721 cgggcgtttt ccgactggca ggtcacctgt aataaccaaa acttctgcgt ggcgcgtaat
    18781 acgggcgatc ataatggact ggtgatgacc ctgagccgca gcgccggggc gcataccgat
    18841 gccgttttac gtattgagcg cggcggattg aagtcgccgg aggcgtcaga aggggagata
    18901 gcgccacggc tgctgttaga tggcgagccg ttggcgttaa gtggtgacaa gtggcggatt
    18961 tcaccatggt tattagtaac cgatgatacg gcaaccatca ccgcgttttt gcagatgatt
    19021 caggaaggga aggcgatcac cttacgcgac ggcaatcaga ccatttcgct gagtggctta
    19081 aaagcggcgt tgttgtttat tgatgctcag caaaagcgcg ttggcagtga aaccgcgtgg
    19141 atcaagaaag gggacgaacc gccgctcagc gtaccgcccg cgcctgcgct gaaagaggtc
    19201 gcggtggtta acccaacgcc gacgccactc tcactcgaag aacgcaacga tttgctggat
    19261 tatggaaact ggcggatgaa tggtctgcgc tgctcgcttg atccattgcg tcgtgaggtg
    19321 aatgtcactg cgctgactga tgataaagcg ctgatgatga ttagctgtga ggcaggggcc
    19381 tataacacca ttgatttggc atggattgtg tcgcgtaaaa atccgctggc ctcgcgcccg
    19441 gttcggttgc gtttgccgtt caacaacggg caggagacga atgaactgga actgatgaac
    19501 gcaacatttg atgagaaatc gcgtgaactg gtgaccttag cgaaagggcg cggattaagc
    19561 gattgtggca ttcaggcgcg ctggcgcttt gacggtcagc gttttcgcct ggtgcgttac
    19621 gccgcagaac ccacctgcga taactggcat gggccagacg cctggcccac gttgtggatc
    19681 acccgttaaa caaaatggcg gggacatgta ggccggataa gacgctgtag gccggataag
    19741 acgcgtcaga gtcgcatccg gcctacagcg tccggcaatc agcatccggc gatcaccatc
    19801 aggcaccttt cactcccagc accttatgcg cttttgccac aatattctcg gcggtaaagc
    19861 caaagaacgg gaacagctta tccgccggag cagattcccc ataacctgtc atcccaacaa
    19921 tcgccccttt caaaccaaca tacttgtacc agtaatcggc aatacctgct tccaccgcca
    19981 cgcgagccgc aacgttagaa ggcaacaccg actcccgata ttcctcatcc tgggcgtcga
    20041 aaatatcagt cgagggcagg gaaactacgc gtacattgcg accttctccc gctaatttct
    20101 ctgccgcttg cagggggatt tccatctctg aaccggtggc aatcaaaata atatctggct
    20161 taccgccgct gtctttcagc acataaccgc cacgggcaat ctcttttacc tgatccggcg
    20221 tacgttccac ctgggccaga ttctgccttg agaggatcag tgccgtcggt ccgttgtggc
    20281 gctcaaccgc cagcttccag cccaccgccg cttccacctg atcgcacggt cgccaggtgc
    20341 tgaaatttgg cgttaagcgc aggctggcca gttgctcaac agcctggtgc gtcggaccat
    20401 cttcgcccag gccaattgag tcgtgggtat aaaccataat ctgccgcgct ttcatcagtg
    20461 ccgccatccc cgcggcgtta cgggcgtatt caacaaacat caggaacgtg gcggtatacg
    20521 gcacaaagcc gccgtggtgc gcgatgccgt tggctatagc ggtcatgcca aattcacgca
    20581 ccccgtagtg aatgtagttg cccgccggat cttccttcag cgaaacagaa cctttccaga
    20641 tggtcaggtt gctggagcca gatccgccga accgccgagc aactcaggca gcatcggc
//
LOCUS       DAJTNN010000062        20647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTNN010000062 DAJTNN010000000
VERSION     DAJTNN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.062"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..877
                     /locus_tag="OO150_001990"
     CDS             <1..877
                     /locus_tag="OO150_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCR5516150.1"
                     /translation="DQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQ
                     YGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKG
                     FSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTN
                     IINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAED
                     GGFTLEINGSEKELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQP
                     KLRGNVQTAQREVAP"
     gene            874..1963
                     /locus_tag="OO150_001991"
                     /pseudo
     CDS             874..1963
                     /locus_tag="OO150_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(2051..3349)
                     /gene="exuT"
                     /locus_tag="OO150_001992"
     CDS             complement(2051..3349)
                     /gene="exuT"
                     /locus_tag="OO150_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5516151.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3832..5244
                     /gene="uxaC"
                     /locus_tag="OO150_001993"
     CDS             3832..5244
                     /gene="uxaC"
                     /locus_tag="OO150_001993"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5516152.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5259..6746
                     /gene="uxaA"
                     /locus_tag="OO150_001994"
                     /pseudo
     CDS             5259..6746
                     /gene="uxaA"
                     /locus_tag="OO150_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6829..7380
                     /locus_tag="OO150_001995"
     CDS             6829..7380
                     /locus_tag="OO150_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5516153.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDDRRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7385..8629)
                     /gene="sstT"
                     /locus_tag="OO150_001996"
     CDS             complement(7385..8629)
                     /gene="sstT"
                     /locus_tag="OO150_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5516154.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9245..10210)
                     /gene="alx"
                     /locus_tag="OO150_001997"
     CDS             complement(9245..10210)
                     /gene="alx"
                     /locus_tag="OO150_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5516155.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINARVNYRHDKQRGE"
     gene            complement(10493..11479)
                     /locus_tag="OO150_001998"
     CDS             complement(10493..11479)
                     /locus_tag="OO150_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5516156.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11558..12241)
                     /locus_tag="OO150_001999"
     CDS             complement(11558..12241)
                     /locus_tag="OO150_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5516157.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHSQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12318..12821)
                     /locus_tag="OO150_002000"
     CDS             complement(12318..12821)
                     /locus_tag="OO150_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5516158.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12906..14042
                     /gene="rlmG"
                     /locus_tag="OO150_002001"
     CDS             12906..14042
                     /gene="rlmG"
                     /locus_tag="OO150_002001"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5516159.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14327..14641
                     /gene="higB"
                     /locus_tag="OO150_002002"
     CDS             14327..14641
                     /gene="higB"
                     /locus_tag="OO150_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5516160.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14638..15054
                     /gene="higA"
                     /locus_tag="OO150_002003"
     CDS             14638..15054
                     /gene="higA"
                     /locus_tag="OO150_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5516161.1"
                     /translation="MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(15099..17117)
                     /gene="fadH"
                     /locus_tag="OO150_002004"
     CDS             complement(15099..17117)
                     /gene="fadH"
                     /locus_tag="OO150_002004"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5516162.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSESGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELNARRAIAQGTRLALEI"
     gene            complement(17543..19894)
                     /gene="ygjK"
                     /locus_tag="OO150_002005"
                     /pseudo
     CDS             complement(17543..19894)
                     /gene="ygjK"
                     /locus_tag="OO150_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19911..20647)
                     /gene="ygjJ"
                     /locus_tag="OO150_002006"
                     /pseudo
     CDS             complement(19911..20647)
                     /gene="ygjJ"
                     /locus_tag="OO150_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YgjJ"
BASE COUNT         5033 a         5413 c         5426 g         4775 t
ORIGIN      
        1 cgatcaaacg tcatggcagt tatcgcatac cttttataat gattcattca gccataaaga
       61 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
      121 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
      181 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
      241 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtat tggtggatgt
      301 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag ctcgggtggc
      361 aggcagccga actttgcagc ttggtcaaag cgacagtgtg ttgttccctt atcctggatt
      421 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
      481 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
      541 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
      601 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
      661 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
      721 ccttaagtgt ccactgagtg ttgaaaagaa acgcgccagc attcgttaca gcggagatgt
      781 tacttgtagt gtggtgactt attcacaatt accggagttg aaacagccta aattacgtgg
      841 aaacgttcag acggcgcaaa gggaggttgc accatgagaa atcgattgat tgcggcgata
      901 ttgggcttgt ttggcacgct cactggcgtt caggcagctc ctgacgtgac cagtgaaatt
      961 acgtatgatt tggcatctgg tagagcggat tattacttct ggaaggatga agcgtcggca
     1021 ggaaataatg gatatatgtg gtatgaatgt tcgtatcctg acctccaaca aacctgtaca
     1081 gctaatggaa atatatcgac agtacaaatc tatttaactg aacaacgcag tgggatgcgt
     1141 tggccggtaa aactcaaagg atttaaaaca gccattgtaa gtagcgatga agcgccgcta
     1201 ggatgcaagg gggggcaaag ggcttcagac gaatcttaag gattctaata gatcttcatg
     1261 tacagaagat ggtcaacatt attatatata cgatacaaag tttcttacgc tctaccttga
     1321 gcagacagag atgaagaatt tgccgattgg tggcgtctgg aaggggaaag ttaaattaca
     1381 ttcgaacagc ccggcccagg actatttcgc aaatattacc ctgaatacgc tcgaccccaa
     1441 ccatattgac gtgttcttcc cggagttcgc ccacgccacg ccgagggtgc agttagactt
     1501 gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa gatctgacca tgttggacat
     1561 gtgcctgtac gatggtttta acggtaatgc catcagttat gaaatcatgc tcaaagatga
     1621 agggcgacca gctgcagggc gcagagacgg tgacttctct atctatcgtc agggaggaac
     1681 ctctaccgac gagggagaac gcattgatta ccgggtcaaa atgtacaacc cggaaaccgg
     1741 tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg aacagcatta acctgaaaag
     1801 tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg atgtgtgtgc cgacaccctt
     1861 ggctctggca gtcgataaat tcagcgtgat ggataaacag gccgggtact acatgggcaa
     1921 attgtcagta atctttacgc cttccttgcc aaccatcaat taaccctgca aagcggacaa
     1981 acgctttttt ctatataaac gacgaagccg cctgataagg cggcttcgtc gttataactt
     2041 acgttaaacg ttaatgttgc ggtgcgggat cgttatgtga ttcctgcgct acctcaatcg
     2101 ccggtttgtt ctgcaacacg gtccagataa ccagcgcacc taacaggtcg aacactgcca
     2161 gtactgcgaa cagcgggctg aagccgatgg tgtcagccag tgcaccgaca accagcgaaa
     2221 acagcgtact tgccagccat gcggacatcc cggttaaacc gttcgccgtc gccacttcgt
     2281 tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc agacagtgcc tggtgagcaa
     2341 aaccaccgat acacagcagc ataattgcga catacgggtt ggtgaacagg ccgatcatac
     2401 ccgggccaat catcagcact gcacccagcg ttacgaccat cttacgggaa acgatcaggt
     2461 tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc accgaggata caaccgaggt
     2521 cagcaaacag catcggcatc caggcgaaca tcgcgatctc tttcaggtta aagccgtaaa
     2581 ctttaaacat gaacagcggg atccacgcgt taaaagtacc ccaggctggt tctgccagga
     2641 aacgcggcag cgcgataccc cagaactgac ggttacgcag gatctgacca acggacattt
     2701 tcttcgccgt gctcacctgg tgctgggctt cctgaccatt aataatatag tcgcgttctt
     2761 catcggtcag atgcttctgg tcgcgcggat gtttatagaa aatcagccat gccatcgccc
     2821 agataaagct caatgcaccg gagatgataa atgccatctg ccagctgtgc attacgattg
     2881 cccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtaac
     2941 ctaccgcgat ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg
     3001 ggatcatcgc tgcttccgcg gcaccgactg caccacgagc aatcgccagg ccacccctgc
     3061 taccagccag cgcggttgca ccacagaata cggcccacag tacagcaaac attgcataac
     3121 cgattttcgt acccagcaca tccagtacat agcccgctac cggttgcatg accgtataag
     3181 cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca
     3241 tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccaa
     3301 gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca
     3361 tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag
     3421 ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt
     3481 gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat
     3541 ttgcattccg tcgacttatg atttgcgacg gcagaaagat gacttgtcat acaactttaa
     3601 aaggtgagag ccatcataaa tgtgcgaata tttgtaggga cattacctga cgacagcgag
     3661 gccagtactg gcgcggcctg cagcgagatt taccactttg agagtaattt ttttaactac
     3721 gtttattgat ctaactcacg aaaatatctt cggactctgg aaattggtgt gataactttg
     3781 tcagcatcgc accataagca agctagctca ctcgttgaga ggaagacgaa aatgactccg
     3841 tttatgactg aagatttcct gttagatacc gaatttgccc gccgtctgta tcacgactac
     3901 gcaaaagacc agccgatttt cgattaccat tgccatttgc cgccgcagca gattgcggaa
     3961 gactatcgtt ttaaaaacct gtatgacatc tggctgaaag gcgatcacta caaatggcgc
     4021 gctatgcgta ccaacggtgt ggccgagcgt ctgtgtaccg gtgatgcgtc tgaccgtgaa
     4081 aaatttgacg cctgggcggc gactgttccg cacactatcg gcaacccgtt ataccactgg
     4141 acgcacctcg aactgcgtcg tccgtttggt atcactggca aattgctttc tccgtcaact
     4201 gccgatgaaa tctggaacga atgtaacgaa ctgctggcgc aggataactt ctccgcgcgc
     4261 ggcatcatgc agcagatgaa cgtgaaaatg gtcggcacca ccgatgaccc gatcgattct
     4321 ctggagcatc acgcagagat cgccaaagac ggctctttca ccatcaaagt gctgccgagc
     4381 tggcgtccgg acaaagcctt caacatcgaa caggcgacct ttaacgacta catggcgaag
     4441 ctgggcgaag tttccgatac cgacattcgc cgctttgctg acctgcaaac tgccttgacc
     4501 aaacgtctgg atcacttcgc cgctcacggc tgtaaagtgt ctgaccatgc gctggacgtg
     4561 gtgatgtttg ctgaagcgaa cgaagcggaa ctggacagca ttctggcgcg ccgtctggct
     4621 ggcgaaaccc tgagcgagca cgaagtggca cagttcaaaa ctgcggtact ggtgttcctt
     4681 ggtgccgaat atgcacgtcg cggctgggta cagcagtacc acattggcgc gctgcgtaat
     4741 aacaacctgc gtcagttcaa actgctgggg ccggatgtag gctttgactc catcaacgac
     4801 cgtccgatgg cagaagagtt gtctaagctg ctgagcaagc agaacgaaga aaacctgctg
     4861 ccgaaaacca ttctgtactg cctgaacccg cgcgataacg aagtgttggg caccatgatc
     4921 ggtaacttcc agggcgaagg tatgccgggc aaaatgcagt tcggttccgg ctggtggttt
     4981 aacgatcaga aagacggtat ggaacgtcag atgacccaac tggcgcagct cggtttgctg
     5041 agccgctttg tcggtatgct gactgacagc cgtagcttcc tgtcatacac ccgtcacgaa
     5101 tacttccgcc gcattctgtg ccagatgatc ggtcgctggg tggaagcggg cgaagcaccg
     5161 gcggacatca acctgctggg cgagatggtg aaaaatattt gctttaacaa tgcgcgtgac
     5221 tacttcgcca ttgaactgaa ctaaggtctg gggttgatat gcaatacatc aagatccatg
     5281 cgctggataa cgtcgcggtc gctttagcgg atttggctga aggcacagaa gtcagtgtcg
     5341 ataaccagac tgttacgctg cgccaggatg ttgctcgtgg acataaattt gcgttaacgg
     5401 atatcgcaaa aggggccaat gtcatcaaat atggcctgcc gattggttat gcattggcgg
     5461 atattgcggc gggggaacac gtccacgccc acaatacgcg cacgaatctg agcgatctgg
     5521 atcagtatcg ctatcaacct gattttcagg atctgcctgc gcaagcggca gatcgtgaag
     5581 tgcagatcta tcgtcgcgct aacggcgatg tcggggtgcg taacgagctg tggatcctgc
     5641 caaccgtggg ctgtgtcaac ggcatcgcgc ggcagatcca gaaccgtttt ctgaaagaga
     5701 ccaacaacgc tgaaggtacc gacggcgtgt tcctcttcag ccacacctac ggctgctcac
     5761 agctgggcga cgatcacatt aatacccgca ccatgctgca aaacatggtg cgccacccga
     5821 acgcaggcgc agtgctggtg attggtctgg gctgtgaaaa caactaggtt gccgcattcc
     5881 gtgaaacgct gggcgatatc gatcctgaac gcgttcattt catgatctgc caacagcagg
     5941 atgatgagat cgaagccggg atcgagcatt tgcatcagct gtataacgtg atgcgcaacg
     6001 ataaacgcga gccaggcaaa ctcagcgaac tgaagtttgg tctggagtgc ggtggttctg
     6061 acggtctttc tggtattact gctaacccga tgttggggcg tttctctgac tacgtgattg
     6121 ctaacggcgg tactaccgta ctgaccgaag tgccggagat gtttggcgca gagcagttgc
     6181 tgatggacca ttgccgcgac gaagcaacgt ttgaaaaact ggtcaccatg gtcaacgact
     6241 ttaaacagta ctttattgcc catgatcagc cgatctacga aaacccatca ccggggaaca
     6301 aagcgggcgg tatcaccacg ctggaagaca aatcacttgg ctgtacccag aaagcgggtt
     6361 ccagtgtcgt ggttgacgtg ctgcgttacg gcgagcgtct gaaaacgcca gggctgaact
     6421 tgttaagtgc gccgggtaac gatgccgtag cgaccagcgc cctggcgggt gcgggctgcc
     6481 atatggtgct gttcagtact ggtcgcggca cgccatacgg tggatttgtg ccgacggtga
     6541 aaatcgccac caacagtgaa ctggcggcga agaaaaaaca ctggctcgac tttgacgcgg
     6601 gtcagctgat ccacggtaaa gcgatgccgc agttgctgga agaatttatc gacaccatcg
     6661 ttgagtttgc caacggtaaa caaacctgca acgagcgtaa cgacttccgc gaactggcga
     6721 tcttcaaaag cggcgtaacg ctataaagtc gaaatgagtt gttaataaaa aacggcgttt
     6781 cataatgtga agcgccgttt gcttttccat acctgataag gatttatcat gaccgcgtat
     6841 tggctggccc agggcgtggg tttcatcgcc tttctgattg gtatcacaac atttttcaat
     6901 cgtgacgacc gtcgcttcaa aaagcagctt tcggtctata gcgccgttat tggcgtacat
     6961 ttttttcttc tgggcaccta tcccgctggt gccagtgcca tccttaatgc cattcgtaca
     7021 ttgattacct tacgcacgcg cagcttatgg gtaatggcga tttttattgt gctgactggc
     7081 ggaattggcc tcgcgaagtt ccatcatcct gtcgaactat tgccggttat cggtacgatt
     7141 gtcagtacca gggcgctgtt ccgctgtaaa gggctcacca tgcgctgcgt gatgtggttt
     7201 tcaacgtgtt gctgggtgat tcacaacttc tgggcggggt cgataggcgg cacaatgatt
     7261 gagggtagtt ttctgcttat gaatggcttg aatatcattc gtttctggcg gatgcagaaa
     7321 aggggaattg atccgtttaa agtagagaaa acccctcccg ccgtagacga aaggggttaa
     7381 acaattaatt acgcagggcg ctatttgcca gacgatcgtc ttccgcctgg caagctgccg
     7441 cagtgaacag cacgtcagtt gaagagttca gcgcggtttc gcaagagtcc tgcaatacgc
     7501 cgatgataaa gccgacggca accacctgca tggcgatatc gttcgaaata ccgaacatat
     7561 tacaggccag cgggatcagc agcagagaac cccccgccac gccggatgcg ccacaggcac
     7621 acagagaagc caccacgctc aacagcagcg ccgtgggcag atcgaccggg atacccagcg
     7681 tattaaccgc agccagcgtc aacacggtaa tggtgattgc tgcgcccgcc atattgatgg
     7741 tggcacccag cggaatagaa acggaatagg tatcgcgatc cagattcagc ttttcacaca
     7801 gtgccatatt caccggaatg ttcgccgcag agctgcgggt gaagaaggca tacacgccgc
     7861 tttcgcgcag gcacagcagc accagcggga acgggttacg acgaattttc caccacacca
     7921 gcaatgggtt aaccaccagc gccaccagta acatacagcc aaccagcacg accagcagtt
     7981 gcgcgtagcc ccacagtgtg gagaaaccgg tggttgccag ggtagaagaa accagaccaa
     8041 aaataccaat cggtgcgaag cgaatgacca gtttcaccat aaaggtgacg gcattcgaca
     8101 gatcgttgac caggtttttg gtggtctcgt taccgtgacg cagtgcgaaa ccgaggccaa
     8161 ttgcccacac caggatcccg atgtagttac ctttcagcag cgcgtcgatg gggttggaaa
     8221 ccatgctcat taccagcccg cgcatcactt ctacaatgcc tgacggtggc gaaatatcac
     8281 ccgcgctact ggataagtgc agggtagaag ggaaggcgaa gctgaaaacg actgcggcca
     8341 gagcagcaga gaaggtaccc agcagataga ggaacaaaat agggcggata ttggttttct
     8401 gcccgtgctg gtggttagca atagatgcca tcaccagcat caacaccagg atgggggcaa
     8461 cggctttcag tgcgccgacg aacaaagtac ctaacagacc aacagcttcc gccgcgggtt
     8521 ttgagatcca tgccagaaga atccccagaa caaggccgac caggatttgt tttaccaggc
     8581 tgccatgagc cagacgccgg aatagccccg gtgaacgttg cgtagtcatt tttcgatcct
     8641 ttcattgtgt tgtcgcacat ccctggtgcg ttctgtcgac gcatctttca ggatgtaaca
     8701 aagtgtttgc aaggtcgagt ataaggaaag ttacagcttc gggaagcgaa aaatgctgga
     8761 ttttaaagag aggtcatatt ttgcaactta attgttacat ttgtttgaca taaacagagg
     8821 gaaggactgg ctactaaggc tgattagata acatatgggc aaaactattt ggaatatgag
     8881 aacggttgcg ctcctttaat gtgctatgcc atataaagtc actggcgcag ggagcgcgca
     8941 gggggcagcc aatcgccgcc gccccctgca cccccaggct ctggcgaaca aaatcgccgc
     9001 tgcgcgattc cctcagctta tcccttgcgg ctaccgggtc gggcgcaagg gaacgtccct
     9061 gtaagatgcg ccctcgccgc gcatccatgc gcgtcgcccc ggcctccagg gaacgcttcg
     9121 gcgattttga cgccaccaac acccgtgcgg tctttttgta aagagctaca agccaattac
     9181 ataattaata gctcaccttt gtctgggatg agttatctct aggaatggct taatatacag
     9241 cgaatcattc tccccgctgc ttatcatgcc gataattcac cctggcgttg ataataaacg
     9301 tcatcaccag aatgccaaac accacgccca gcgagacggc gattggaata tggtagaagt
     9361 cgacaatcag catcttgata ccgataaaca ccaggatcac cgccaggcca tatttgagca
     9421 tcgagaaacg ctctgctacg cccgccagca ggaaatacat cgcacgcagg ccgaggatcg
     9481 caaacaggtt tgaggtcagc acaatgaacg ggtcagtggt cacggcgaag atagccggaa
     9541 tgctatccac ggcgaaaatc acgtcgctca attccaccag aatcagtacc agcatcagcg
     9601 gtgtggcgta cagcaatcca ttcttacgca caaagaaatg ctcgttgtcg atggtgtcgg
     9661 tcatgcgcaa atgaccgcgt agccagcgca ccagcggctt gtcgccaatg cctgattcat
     9721 cttcatgggc gagcgccatc ttcacgccgg taaacagcag gaaggcaccg aagatataca
     9781 ggatccagtc gaactgcgaa attagccagc tgccagtgaa gatcatgatg gtacgcagaa
     9841 caatcgcccc gagcacgcca tacaccagca cgcggcgttg taatgccgcc ggaacagaga
     9901 aatagctgaa caacatcagc cagacaaaga cgttatcgac cgccagcgat ttctcaatca
     9961 gataacccgt gagaaaggcc agtgcctgtg gatcggcaac ggcgcgacct tcggtttgca
    10021 ccagatacca ccagaaagcg gcgttaaaca gtaacgacag cgtcacccaa accagcgacc
    10081 aggccgcagc ctgtttcatg gtcatggcat gtgccccacg acgcccctgc aacaacaggt
    10141 cgatagccag cataatggcg acaacaacag cgaatccgcc ccatagcaac ggcgtgccga
    10201 cagtattcat agaagttcct tacacataaa aaaaacggcc aacgtcggaa gacgtcagcc
    10261 gctgcttttt atgcatagac ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa
    10321 tacgtctgcg tattcctttc gggttgcccg gtgaccggat gtggtttttc acacatcgta
    10381 atgacgatcg accggcaatg aagttactcc cctttgcagg taacaaagta tgacagccat
    10441 tcagccagtc aatggtctgt tacatacctt ttacactgtt ttactttggc aattatagtt
    10501 ttacgctatc tgccggaaaa atcaccccag tctggcggcg gatctcagtc agcagtttgg
    10561 cggtgatgcg actgaccgcc agccctggat gatccaccag atgctcatcc accagggtag
    10621 caaacagctc tgcttcgtag agcatggtat taatatgctg cggctgggtg agatcctgca
    10681 tctggctgcc acgcggcacg aagcacactt tctggcattc agacagtttt tcaatcacca
    10741 gcgatcccgc ttcgccctga atctcgcttt ccaggacaga atcactgacc tttgagtgca
    10801 gcaacgtaac gctgaaatca ccgtaatcca tcaccaccac gccgtgggca tctacgccgc
    10861 ttgccaacaa actggcggtt gcctgcacgc ttttcggttc accgaataat gccaccgccg
    10921 acgccaggca gtaaaagccg atatccataa ttgaaccgtt agagaatgcc ggattaaagg
    10981 tgttgggatt ctcaccatcc aggtaacgtt gataccgcga ggaatactgg caatagttga
    11041 aaaagacttt acgcaatttg ccgactttcg gcagcgcctg gcgcagcaaa tgaaagttcg
    11101 gcaggctggc ggttttaaat gcctcaaaca gcaccacctg attttcccgc gcacaggcaa
    11161 tggcggcatc cacttccgcc agattcgacg ccagtggttt ctcgcaaatc acatgaattt
    11221 tatggctaag gaaaagttgt gtctgggaaa agtgcagggc attcgggctg gcaatataca
    11281 ccgcgtcaat ggcatcgctt tccgccatcg cttccagcga ggtaaacaga tgctcgacag
    11341 aaaaatcatt ggcgaagtgc tgggcctgtt caaggctgcg ggaatatacg gcggttaact
    11401 tgtatttacc gctctcatgg gcggcctcga cgaactggcg agtgatccag ttcgtaccaa
    11461 tcacagcgaa acgtatcata acgcgtggat actccataaa gggattctgt cgtcaatgta
    11521 gcacgccttt ttcatcttcg acgtgcgcag atacgcatta ttcttttgcc ggacagccat
    11581 gctcactaaa cggcccaatc atgaccgatg gcccgagata tttcggctgg ctatggagga
    11641 ttttggcgtc aatcacggcg ctgacacgag cgagtttctc tcgcagttga gtataaaaac
    11701 cgtgcttcat attgaggtca ggcgcgttaa aaccgatagc gtcgatgctg tactgttttg
    11761 ccagccagat ggcgcgttgg ttatgaaatt cttgcgagat aatggtgata tggttttcac
    11821 caaaaacctt tttggcacgc actaccgaat ccagcgttga gaatccggca tagtcgcaga
    11881 agatcacttt cgccggtacg cctttagcga tcaaagcctt ctgcatacct gatgcttcat
    11941 cgtaattttt acgtccgtta tcgccactta ccagtagcca tttcactttt ccggcgtggt
    12001 acaacgctgc tgcggtatca atacgtcggg taaagtagcg attacccggc ctggccccca
    12061 gcaataagcc cacgttacgc gccggaactg cgttgacatc gctccaggtc agctgcttgc
    12121 tggcgttgac cattactcga tcggcgataa aaatggtcgc tcctgccaca agtaacagta
    12181 agcaggcaat ctttagagtc cggcgagaaa aacaaatacg gagaagaagg cggggctgca
    12241 tggtcgttat ccttaacgaa aggggggagg tcgattttag gcgctaaaga aagaaaagat
    12301 aacgcctgac atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc
    12361 cgggtgtcga actccagctg gtggtactgc ggctccatat ggcagcacaa ctggtaaaac
    12421 gctttgttat gctctttctc cttcaggtgc gccagttcgt gcaccacgat catacgtaaa
    12481 aacggttccg gcgcgttgcg aaacacagta gcgacgcgga tctctgcttt tgctttcagc
    12541 ttgccgccct gcacacgaga aacagcggta tgtaacccga gcgcgttttt tagcacatgg
    12601 atcttattgt catacatcac tttattgatc ggcggggcat tgcgcagaaa ctgatttttt
    12661 agatcctgag tatattgcca gagggcttta tcggtggcgt agtcgtgtgt tcccggatag
    12721 cgttttgcca gcacatcgcc cagacgctgt tcgttaatca gcgttcgcac ctgggaaagt
    12781 agctgctcgg gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag
    12841 aaaaagggta tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag
    12901 ggccgatgag ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga
    12961 cggatgacgt taacccgcta caggcgtggg aagcggcgga tgaatatttg ctgcaacagt
    13021 tggacgacac agaaatccgc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa
    13081 gctgcgcgct ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg
    13141 cgacgcgcga gaatttacgc ctcaacggga ttgatgagtc gagcgtgaag tttctcgaca
    13201 gcaccgccga ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg
    13261 cattgctgga acaacaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta
    13321 ttgccggtgc caaagcccgt gacattcaca cttccacgct ggaactgttc gaaaaagtgc
    13381 tcggcccgac caccaccacg ctggcatgga aaaaagcgcg cctgattaat tgcacgttca
    13441 atgaaccgcc gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact
    13501 ggactatcca taaccatgcg aatgtcttct cccgcaccgg gcttgatatc ggcgcgcgct
    13561 tctttatgca acatctgcca gagaatctcg aaggggagat tgtcgatctc ggttgcggta
    13621 atggcgttat tggtctgacg ctgcttgata aaaatccgca ggcgaaagtg gtgtttgtcg
    13681 atgaatcgcc gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg
    13741 cgttggatcg cagcgagttt atgatcaaca acgcgctctc cggcgtggag cctttccgct
    13801 ttaatgctgt gctctgcaac ccgccgtttc accaacaaca tgcgctgacc gataacgtcg
    13861 cctgggagat gttccaccat gcccgccgct gcctgaaaat caacggcgag ctgtatattg
    13921 ttgccaaccg tcacctggat tacttccata aactgaagaa gattttcggc aactgcacca
    13981 ccatcgccac gaataataaa tttgtggtgc tgaaagcagt gaagctgggg cgtcgtcggt
    14041 aagattggga gttacctgcc ggatgcggcg ttaacgcctt atccggccta cgttcagcac
    14101 gttactccca ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgcccagttc
    14161 tggaatcgcg cttccataaa acaaaaaatc cctctcacca tcctctacat tctcttgttt
    14221 agcgtttttc tacgtttatt cttccgtcac acagataaat tcatacgttg ccaatctgtc
    14281 aacggatgtt atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac
    14341 tcaaaaagca ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc
    14401 tctggggaac acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt
    14461 cccatctctg gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa
    14521 tgaattacgt gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt
    14581 tatgacgcat aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg
    14641 attgcgattg ccgacatctt gcaagcagga gaaaagctaa ctgcggtggt accttttctg
    14701 gcgggtattc agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg
    14761 ctcaacgatc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa
    14821 gaatcagcgc ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata
    14881 gccgtgattc gtacccttat ggatcaatat ggtttaaccc ttgccgatct gccggaaatt
    14941 ggcagtaaat ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac
    15001 gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac
    15061 gtgccggatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt
    15121 gttccctggg caattgcccg tcgtgcgttc agctccatag ccacatcgca gccgccaatt
    15181 aaatgcacag ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc
    15241 cctgcgcaga tcaccacatt gtccacagct aatacctgcg tttcgccgtt gatcaccaca
    15301 tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc
    15361 gagagcaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg
    15421 ctggctttgc gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc
    15481 gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg
    15541 gcgatattct ggctggttga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca
    15601 atcccgccac aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca
    15661 tcgagataac tcaatacctt cggatgatcg atcccgtcga taggcggaac gcgcggcacg
    15721 atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga
    15781 ttgagtttca gcgtcacgcc cgtcacttcg atcatccggt ggtaatagcg cagcgtttcg
    15841 taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc gctctcgctg
    15901 tgggcatcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc
    15961 ccagcaggtc ccgcaccgac caccgccaga ttttttttct gcacggcagg aaggattggc
    16021 attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag
    16081 atctgatcga ggcaggcctg attgcagcca atgcaggtgt tgatctcatc ggctcgcccc
    16141 gattgcgctt ttgacagcag ttccgcatca gcaagaaacg gtcgtgccat cgataccata
    16201 tcggcatcgc cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg
    16261 gttaccagcg gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg
    16321 cgcggcacgg gcgtggcaat ggtcggaata cgtgcttcat gccagccaat gccggtgttg
    16381 ataatggtcg cgcccgccgc ttcaatggcc tgcgccagct ctaccgtttc ggcaaaagtc
    16441 ccgccgtctt cgaccaggtc gagcatcgac agtcggtaga taataatgaa gtcgttgccg
    16501 acgcgttcgc gcaccgcacg cactacttct acggcaaatc gcatccggtt gcggtaatcg
    16561 ccgccccact ggtcactacg ctgattggtg tgcagcgtca gaaattcgtt gatcaaatac
    16621 ccttcggacc ccattacctc tacaccgtcg tatcctgcct cccgtgccag ttgcgcgcag
    16681 tgggcgaaat cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa
    16741 cggttgatgg gggcctgcaa tgcggaggga gcgaccagat gcggttggta gctgtagcgc
    16801 ccggtatgca aaatttgcag ggctattttg ccgccttcct gatgtaccgc ttcggtaatg
    16861 gtgcgatggt gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg
    16921 cctgttaaat ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt
    16981 tcggcataaa acgctgccag ccgctcggca ccgtccgggt attcctccag cccggtgtgc
    17041 attgagccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac
    17101 agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt ggtcggatga
    17161 gttctaattt agccgtcgcc ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga
    17221 ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt tttatccggc
    17281 ctgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agtggcgcgg
    17341 tgcagatgcc ggatgcgacg cttgtcgcgt cttatccgac ctacgggaac gcacatgtag
    17401 ggcggataag gcgttcacgc cgcatccgcc agtggcgcgg tgcagttgcc ggatgcaacg
    17461 cttgccgcgt tttatccggc caggaggatt gcacatgtag ggcggataag gcgtttacgc
    17521 cgcatccgcc agcagttgca aattattgct tacggaaaaa atcgttatac aacatataca
    17581 aatgcgcggc actccaggag aaatttggtg cgccttgctg cgcgccagtc agcgggttgt
    17641 aattctcctg aattgggcca tcggcggtta atcctttggc gtgccggaag aacgtatccg
    17701 ccagcttcag ggcatcatca cgataaccgt aacgctccat ccctttcaga gcaaaccaga
    17761 actgatccac ccatacgcgc ccgcgccagt agatatcagc gccaaaagcc ggattggtta
    17821 acgccgccgt tcccagcggg acaaaggtgt tgaactcttt aggatcgagc atcaccttca
    17881 ccaccgcgtc ggcattggcc tgcgttgccg caccgttaaa cagcggcgac cagccttccg
    17941 gccctttacc gcgctcaaca atcggtttgc ccgcgcagcc gttcgccagt ggtttatctt
    18001 caatacgtac gtcatagtag aactgggtag tcgggtcgaa catacaggtg ttgatgtagt
    18061 ccgcgagctg ctgtgccaac tggcgatagc gtttggcctc ttctggctta ccgaggatgg
    18121 ttgccatctc cgccagataa tggttatcgc tgtacatata gctggcctga tccaccgact
    18181 cctgcaatag cgagtagccc agcaacgttc cgtcctgact gcggttttcg gcgaatttca
    18241 ccgtccagtc gctacgtttg ccaccattag cgacatattt atccaactgt tctttgtcga
    18301 taaaaccaaa gacggcggca tcatcacgcc ccgattccca cgacgcagcg acctgtgccg
    18361 gaatttccag actgtcgtac tggcctttct ccaccacgcg ggcgtagttg ttcagcccag
    18421 actgcgtctc ttctttgttg cctttcttca ccgcaaacag catctcgccg ctctcagtgt
    18481 tgtgggcttt gtcgcgggtc gcgccatatt ccggcacgcc gttgccgttg tgatcgcggt
    18541 tacgtaacca ccagtcgtga taggcgacca gtttcgggta catctctgcc aaccaggctt
    18601 tatcctgggt gacgttgtac acttctatca ccgaccaggc ggcaaggctg ggtttggtat
    18661 tacgttcgtt ccagttgccg ccatcgccac cacgctcggg gctaaggttc cacgctatca
    18721 ggtcggggac aaagcccaca tcctgcggac gcacgcggtc gccaggctgg atctgccagg
    18781 agaagaccgc gcggatattc tctttggcga tgtccggatt gaaatgcgcc atcgcaaacg
    18841 cctgcttcta ggtatcccac ggccaggtct gattgccgga gaaccagcgc ccggtcaccg
    18901 acggtgtaac ggtgttaaat ttcaccgcac cgcccggtga gcgccagtta ccgttgagcg
    18961 tttcgatcgc tttcaccgcg acgcgcgtct gttccggcgt cgcatccgga ttggttaacc
    19021 ctttcttcag gtattcttcc cagcgttgct gcgaggcggt gagataaaac gccggacgcg
    19081 ccagaatatc gcggatctgc atttgctctt tgctaacttc ctgagcggtc agcagatggg
    19141 aataggtggt gtagagcgtg gtcgaaccgt tgatatgcgc cttactggta aagcgattac
    19201 cgttgatttc agtctgcacc ggcagggatt tatgcacctg atattccgat tcgccggagg
    19261 tcagcagatc ccaggtggcg cgcactttgc caaaggtgac tttcaggcca tcacgggtgg
    19321 cgctgatttt gcgctgatag tcagggtatt cgccagcaat ggttttatcg gaaagcggtt
    19381 tcccttcttt cgcttccagt ttttccagca gttcgccgtc ccacaccaga tccagcggtt
    19441 tattgctggt gattttggtt tccagtagtg acgtgcgcgg cgtggcgaag cgcagagtca
    19501 tttcgacttg cacatctttt gctgtcagtt tttgcaccag cgcaccggga atactgtatg
    19561 cctccagcgt aaagtcgact ttcttgccgt cctgccagac ggtcaggcgg tcgaaattgc
    19621 tggccataaa gttgatgtac tcttccgtca gcagcgcaac gcccggaaag ccgcccatgg
    19681 tgttagggcc gtctggcaac agatgaccat gccaggctcc gagatcgaaa aacggattaa
    19741 aacgctggtg atcgtcgtaa tcgtaatcct tcatgtactg cggcgcgcca gtacggttaa
    19801 tcacgttttt ataattgtcg gcgttagcgg catgggcgga aaaacttatc agcagagcgc
    19861 aggttactgg cgttaaaata gtttttattt tcatgaaatt acagtcctta ttaccagtag
    19921 aaatattgca tcaggaagat ttggtcgctg ggatcgtgag aggcattttt catgtagaag
    19981 cggctatcaa aagccgtggc gaaacgtccg ccgccgtatt cgtaccagat accaaactgc
    20041 atgaaagagg tggtttcatc gtcagcgtgg tcagggcgat gtttggcgta ggtgtaagcg
    20101 gtggagagat aaacgccctt taacgcttca tacatggcgc tcaccatcat cccactttca
    20161 cttcccggta cgtccagccc gtcggcgcgt ccggtatggt gccaggcgcg agcggcaaag
    20221 ttaggagtta gcaggcccgt gagatagagc tgtcctgtgc cgtcggtgat ttccagaccg
    20281 ttcatccagg tgatgccttc ggcaaggtca tactggatgt aaccgttcat cattgccgga
    20341 taggtgtatt tgtcgtcata gcgatcgtat ttgccaaaat gtagccaggc tttgctttca
    20401 tcatgatgtc cggcaggagt cgcggtgacg ctataacgaa agttgccgct taagttctgt
    20461 accttcagcg cagccataat gtcgcgggta ttgggaatga cgtagcccag atcgggggtg
    20521 aaatcgcccc accattgcag gtcatccagc gaggaatcct aacgcagact gagcatgatt
    20581 tcggtgccat cattggtacg ataaccgcca tagaaacggt taattccgcc ctcgaaacca
    20641 ccccagc
//
LOCUS       DAJTNN010000063        20368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTNN010000063 DAJTNN010000000
VERSION     DAJTNN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.063"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..4180)
                     /gene="mukB"
                     /locus_tag="OO150_002007"
     CDS             complement(<1..4180)
                     /gene="mukB"
                     /locus_tag="OO150_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5516163.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVCADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLR"
     gene            complement(4180..4884)
                     /gene="mukE"
                     /locus_tag="OO150_002008"
     CDS             complement(4180..4884)
                     /gene="mukE"
                     /locus_tag="OO150_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5516164.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(4865..6187)
                     /gene="mukF"
                     /locus_tag="OO150_002009"
     CDS             complement(4865..6187)
                     /gene="mukF"
                     /locus_tag="OO150_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5516165.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLQVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6184..6969)
                     /gene="cmoM"
                     /locus_tag="OO150_002010"
     CDS             complement(6184..6969)
                     /gene="cmoM"
                     /locus_tag="OO150_002010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5516166.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7105..7884
                     /gene="elyC"
                     /locus_tag="OO150_002011"
     CDS             7105..7884
                     /gene="elyC"
                     /locus_tag="OO150_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5516167.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7861..8754)
                     /locus_tag="OO150_002012"
     CDS             complement(7861..8754)
                     /locus_tag="OO150_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5516168.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQEHFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(8908..9654)
                     /gene="kdsB"
                     /locus_tag="OO150_002013"
     CDS             complement(8908..9654)
                     /gene="kdsB"
                     /locus_tag="OO150_002013"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5516169.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9651..9833)
                     /gene="ycaR"
                     /locus_tag="OO150_002014"
     CDS             complement(9651..9833)
                     /gene="ycaR"
                     /locus_tag="OO150_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5516170.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9885..11117)
                     /locus_tag="OO150_002015"
     CDS             complement(9885..11117)
                     /locus_tag="OO150_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5516171.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLALEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11154..12140)
                     /gene="lpxK"
                     /locus_tag="OO150_002016"
     CDS             complement(11154..12140)
                     /gene="lpxK"
                     /locus_tag="OO150_002016"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5516172.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12137..13885)
                     /gene="msbA"
                     /locus_tag="OO150_002017"
     CDS             complement(12137..13885)
                     /gene="msbA"
                     /locus_tag="OO150_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5516173.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(13922..16194)
                     /locus_tag="OO150_002018"
                     /pseudo
     CDS             complement(13922..16194)
                     /locus_tag="OO150_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16401..16685)
                     /gene="ihfB"
                     /locus_tag="OO150_002019"
     CDS             complement(16401..16685)
                     /gene="ihfB"
                     /locus_tag="OO150_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5516174.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16845..18518)
                     /gene="rpsA"
                     /locus_tag="OO150_002020"
     CDS             complement(16845..18518)
                     /gene="rpsA"
                     /locus_tag="OO150_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5516175.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18629..19312)
                     /gene="cmk"
                     /locus_tag="OO150_002021"
     CDS             complement(18629..19312)
                     /gene="cmk"
                     /locus_tag="OO150_002021"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5516176.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VATFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19485..20248)
                     /gene="ycaL"
                     /locus_tag="OO150_002022"
                     /pseudo
     CDS             complement(19485..20248)
                     /gene="ycaL"
                     /locus_tag="OO150_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
BASE COUNT         4903 a         5643 c         4940 g         4882 t
ORIGIN      
        1 cgcgcaggcg gcgagattca tcttcccagc tttgtaccac catcaccaga atcgacatac
       61 cggtaccaat cgcctcaccg gtcgacaatg caccagactc tgcgcgcaac caaccatcgg
      121 aaccacggtt aacctcaact tccatttcca gatagttgcg gtaatccagc agttcttcgc
      181 caatggtctg cggcgtgcgc tgccccatat caatctgcgg attaagacgc tgatacaatt
      241 tcgccaacgc ttccgagaag gtcagacggt tgctgttaaa cagatcctga tgctgctcgt
      301 gctgttcaga gagcacatcc agcagcatgg cgtgcgtttc acgcacgttc acgttgaggc
      361 gcacgctgtt cacctgacca aacgatacgt tctgcaaccc ctggttgagc atacggatac
      421 ggttctgctc gcgctgaatg gttttgcgaa tgatgttcgc cacgctgcgg gaactgatca
      481 ccagtttctg ttcacgggag gttaattctt cggtcagacg gctaagttcg atctccatct
      541 gttcgatagc ttccaccgga tcatcggtac gaataatatc ctgacgaata cgttcacgca
      601 gatgctgata agccgccacg aagaactgaa ttttacgctc cggacgtttc ggatcttccg
      661 acatgcgcag cacgtcgcgc agatgttcgt tatccgccac cgccagacgc agcgcaccca
      721 atgccttatc cgacatagag cgcaaatcat cagcggagag ataagccagc tcacgacggt
      781 gtaagcggcg ctcaacgccg ttatctttca ccatgcgcat caccgcacac cagcccgctt
      841 tggcggtcac tacctgctcg cgcatctcaa agtaatcccg ctccagcttg cgcagtttgc
      901 gggtcaggtt gtccatctcc gcttcgcaga aggtaagcgc tttttccagt tgattgcggc
      961 gtgaacggtt attgctcagt tgcgcatgca gctcgtcacg gcgaatacgc gcccgctctt
     1021 ctgccccgct atcagcacac acgccgatat cctgcaattc acgttgcaga tcgttgagta
     1081 gctctttttt ggtgtcgtaa gaacttttca gcgaagccag cacctggttg tactgactca
     1141 actgcgctgc gtgaccgcgc aacgcttcgc gagcgcgggt acgctccgct tccgcctgtt
     1201 ccagacgttc acgcagtttt tcgttgagat cgctgttacc gctaagcatt tctgccgagt
     1261 cagagtagct aaagtgcgca cgacgctgca ccacttccgt cagggcaaac gcctgctgac
     1321 gggcatcgcg ctgcatctgt tgagagtacg cgtaatcttc ttttaactgt tcgaactgtt
     1381 ccgggtcgct ctgcaatacc gaaacgatcg gttccagttt cgccagttga ttgccaaact
     1441 gctgaacaaa acgcgcggct tcctgggctt catccagacg ttcgcggatt tcatcgacgc
     1501 gatccgccag gctatcatca gccaacaggt tgagacgcgg cagaatgcgg ttcagcgccg
     1561 taacgccctc tttcgcctgc tcaaactgaa tacgctgctg ctggttatca ttttcatgat
     1621 tacttaacgc ccgctccagt tcgacgcgac ggctgttcag ttgacggatt tctgcttccg
     1681 ggtcagactc aaacacaacc gccagatgac tgccgataaa gcggctgaac gcctgatgca
     1741 gacgctgagt tttctgtaca tcaaaggaga gcgtggcgaa gcgttcggaa agcacttcac
     1801 gctcggcatg gaggctttca atacggcttt cacgcgcagc acgaccaaac agcggcactt
     1861 ccgggaaacg tgaataacgc cactgacgat cggcgatttt caccactacc gctttttcca
     1921 gctcatcaac gctgaacacg ctgtcatcga atgactgcgg atctccttcg atcagataga
     1981 gatcttccgg gcaatcggtc aagccatcta ggtgttcagt tacctgtgac agatctggca
     2041 ccacgatggc gtggcgtgac gggccataca gcgctgagaa gtacggcgca tcttccaggc
     2101 taacgtcgtc ataaatttct gacagcagca caccaccaaa acgctccgcc agcgcgttca
     2161 gacgctgatc ttcagagccg ccaggctggc ttaaacgttc gatctcttca tcgacggcgt
     2221 ttttgcgcgc gcccacttca tcgcgttcaa caatcgcctc tcgctcacgc tccagcaact
     2281 gttgcagata ttcggtgacg tcctggctgg aggtaaactc ttcgccgcac tgttcgctca
     2341 actggttgag actgttttgc gctgccagcc aaaccggcgc acgctgcatc aaactctgaa
     2401 tgcgagactg cagctgttcc tgctcctggc gcagtgccat gcgctcttca cgggcgttag
     2461 acacgctatc ggaaagagag gcaatgcgtg cttccagttc ctgatgcagg gcttccagtt
     2521 cgtcgatatc aaaattcttg ccctgacgtt tgcagaaatc tgccagcaga cgctcagctt
     2581 cttgctgctc gcgtagacgc tgttccagtt cgcttaatcg catccgcagc ggctgaacct
     2641 gctctgccag gtgacgctga tcgacccctt cgcgcaatag ttcgcgagcg acatcccacg
     2701 cctcgttacg cgccagtggg ccgttgattg ccaccaccag ctgataagcc tgctcaaact
     2761 ggctgtgcgc ggtttgcgcc atgctcattt tctgctcaag agagagcatt ttttcagtcg
     2821 cttccagctc tttcgcctgg aaggtttcca gccattcggc ggcgctgtcg gcggttaagt
     2881 ccggcagatg gcacagttct ttggcacgat taagcgcagc aatcgcctgg ttatactgga
     2941 tcgcgcgcgt ttgctggacg tccagcgcct gctggtagtc agcaagctgg cttttcagct
     3001 cgtccacttc cagttcggca gcttccgcgc gagcctcgtt ctcttcctgg cgttcgatgg
     3061 cttctgccac cacttcgttt tgctcttcca gacggatctg cagctcatcg agatccgctt
     3121 cgtagcgttc gatcttctcc tgctgacgca gtgcggtttg caccaggttc aggtgatcgc
     3181 tggccgcctg ataatccgct tccagatcgc cttcggcacc gttgtgctct gccagctcac
     3241 gcgccatatc gacgtgtttg tactgctcag ccgccagttg ctgacgcgaa gtatgtaact
     3301 cgcgacgaaa ctccagggct ttgtcgagat ggacacggcg ctcgttggcg tgacgcatgt
     3361 agtccgccgc cacgtagttg gtagcttcgc tgatcagatg cttaaacagg tcacggtccg
     3421 actgggtgac acgaatcgct tccagcgtca tgcggttttc acgcagcgcc gcttccatgt
     3481 cctggaacgc tttacgcacg ccgctgtttt ccggcaacag atagtcgcgc agagaacggg
     3541 taatggcact ggagatcccg ccatacagcg aagcttcgat cagacgatag aatttgctac
     3601 ggtcagatgc ggagcgcaga cgacgcgcga tgatgcccag atcgaacatc agcgagtggt
     3661 aatcggtaat ggagttgaac tgtttaaact gcacgccttc catcgcctcg agcttgtctt
     3721 tcagctcgtt aagcggcagc acgcgcgcct ggcgttcgtt cagggtttcg gtcaccagct
     3781 gtgtcggctg caccgacatc ggcagtccct gaatggcaaa tggtttgata tccactttac
     3841 gatcgcgtcc ggcaacctgt tgcagacgca caccgaccac cacgcgctgg tggcgcgagt
     3901 taatggtgtc gagcatcgaa taacagacac ccgctttcag cttaccgtgc agacctttat
     3961 cgcgcgaacc gctggtggcc ccggcttccg tagtgttacg gaaatgcagc agggtcaggt
     4021 cggggatcag cgccgtaacg aacgccgcca tggtggtgga tttacccgcc ccgttaccgc
     4081 cagaaagcgt cgtgaccagc tcgtcaaggt caaaagttcg ggcaaaaaag ccgttccagt
     4141 taatcagcgt cagtgagcga aatttaccgc gttcaatcat tattcttcct ctccgctatc
     4201 tggctgattc tcttcggttt catcgttgag ttgcagatga ttttcaatcg gcattgcttc
     4261 gccatcacga atcaggcgac gctgtgcttc acggggatcg tcgccagcac gcacatcggc
     4321 tccgaagcgg aacaccgatt cggtaatgcg aaacttgctg ctgtcgtggc ccataaacca
     4381 caccatgcct aaacgacgca gacggttgag cgaagaacgt actttctcct gcaacttctg
     4441 acggtcaacg tctgaaccgg ttgaacggtt gttcaccagt ttcagcagtt ttgcttcatc
     4501 ggccagggtg agcagttcgt cgtacagttc ctgctgggtg aaaatcccct cattcgccag
     4561 ccgttccggg ctgagataga gataacagag gattttcccg accatcatat ccagttccga
     4621 caagacggaa cgcgggataa gagtggtgga acgtgggcgt aaatagaaga acccttccgg
     4681 tgcgcgaata agctcaacgt tataacgcgc gtaaaactct tccagatatt cctgaaaatc
     4741 catcaggaat gcatgattat ccagttcgtc gaggccaata tggcggcctg aacgtaaggc
     4801 gctgtccagc gccggaaata acggattcgc cagcgcctgc gccagcttaa ccggcatcac
     4861 ttgttcaata tttgtcgatg acatgcgcct gtaccttggc tccgtaatca ttaatcggct
     4921 gccatttcgc tggcagtccg gtgaaatctg cttgcgctac gccaagacgt accgcctgat
     4981 caataacaat acgcgcaacg tcaaagtgac gtgcacgcgg atactgtgac agatattcgc
     5041 gtaccaccag accaagatcc agcggcactt gtctggtttt gtacacggca agttgttctt
     5101 cgatgatcgc cgccagctgt tcgcggatct cgttaaactc ttcgtattcc agatcctcag
     5161 gaagttcccc agtcacttct tcatcgcgca gtgccatctc ttcgtcacgc atatccagca
     5221 gacgatcggc attggcataa gttagcgccc acggctcatc aaaataggtt tgtaccgact
     5281 gacgtaaccg ctgagcaaag acgcggtttt tatccatatc gatcgcggta cgaataaatt
     5341 tgtgtacgtg gcggtcgtag ccaatccaca agtcgatgga ttgctggccc caactgataa
     5401 tacgatcgag tttgctctgc agatcgaaca ccagacgatc gacgaaatgc agatcgtcat
     5461 gggtcatcgt cgcatcctga atgcgcaaca gattagcctg caatttgtcg cctgccgctt
     5521 ccagcgtatc ctgcaattca cgcagcgttc cggaagtttc cgaaagcaac aattcacagc
     5581 tggaaatcgc cgcccgccag tctttgttca gcaactgggc gatatcgtcc ttcacctgct
     5641 gctgctgttc gtccatcaga cgttgcgtca ggtcgatact gtcgaaaatt tctgctaccg
     5701 aatatttcag tggcgcatag acattacggt gccagtgaaa ttcatcaccg ccctcttcgg
     5761 cggcatctgc tgcgcgtttg agctcacccg ccacaatcga caactgcata gaaagacgca
     5821 gcgtagaaaa ctcgcgctga cggatgtagt agtcagtaat gccgatgccg agcggcgtca
     5881 gacggtaaat tgcgttccct tccgcctgct cgctggtaaa gcggttcagc agacgttgac
     5941 gcaccatgtc gttgatcgcg ttattggcgc gcacgccgat ggtttcgctg gtttgctcaa
     6001 acgcatcact cacatggcgg aatgcatcca ccagctcgcc ttcactcatc tcaccatcca
     6061 gacgctcgcc gttcagcgtg gcaaccgcca gcagaaaaga gagtcggtct acctgcagcg
     6121 agatggagaa gtcatttttt ctggcccagg caaccagttc ggggactgtc tgggaaaatt
     6181 cactcatact ttatccttgc tctgcggttt gcgcgcggtg acatgaatat aacgccccag
     6241 ggtaatatac ggttcctgac ggcaataacg cgtttctaat tcaagtaatg cttcatagca
     6301 gtcgcgctgc tggtgcttct cgcgcagata atcatgaaac acgcgaacgc ctgtcttacc
     6361 cataatttgc caaccagctt cttccagcca cagataaacc tgcgccgggt cgcgtggata
     6421 atctggcgaa agcgtccgtt ttttcttttt cggcattccc gcctgcacgt aatcaaaatt
     6481 cccggcgacc atgttatgca tcaacaaacc atgcgcattg tagaacatta acgacaacac
     6541 gccgcctgga cgcaacactg accacagggt ctgcaataca ctgcggggat cggccaccca
     6601 ctcgagcacc gcatggaaca atatcagatc aacgggcgtt tccaaatgcg aagcaacatc
     6661 ctgagcggcg caatgtataa attgcatgtt gtcgctcaca ccttttgctt ctgccgcctg
     6721 ttttgcgcgg tcgatcatct gcgcagaaag atcgcataaa atgacctgat gcccacgctc
     6781 ggccattttg attgcggtct gcccttctcc accgccagca tccagcacac gcagtttttg
     6841 cgggcccatt tccgccagca cgcgatcgag atcctgccac agaatagcct gtcgaagctg
     6901 ccctttggtg gtgccgtaaa tgttacggga aaacttttcc gcaatatcat caaaattgcg
     6961 atcctgcatt gctttctcca ccggttggct aagacgtttt ctgataaaga cgctattttg
     7021 tcacagccac ggcgagaatg aacccgtctg gtgtaatatc gctggcgatc ccttgctata
     7081 tggttaaaaa aggaaccaga aaggatgctt tttacactga aaaaagtgat tggcaatatg
     7141 ttgctgccac ttccgctgat gttgttaatt attggcgctg gcctggcgct gctgtggttt
     7201 agccgttttc aaaaaaccgc gaagattttt attagcattg ggtggctggc actcttgtta
     7261 ttgagtttgc aaccggtagc cgaccgcctg ctgcgtccca tcgaaagcac ctatccgaca
     7321 tggaataatt cgcaaaaagt ggactatatc gtggtgctcg gaggtggtta tacctggaac
     7381 ccgcagtggg caccgagctc taatttaatc aataacagtc tgccacgcct gaatgaaggt
     7441 attcgcttat ggcgcgaaaa tccgggatca aaactgatct tcacgggagg cgtagcaaaa
     7501 accaatacgg tgagtacagc ggaagtaggt gccagagttg cgcaatcgct gggcgtgccg
     7561 cgcgagcaaa ttatcaccct ggatttgcca aaagataccg aagaagaagc tgcggcagtg
     7621 aaacaggcga ttggtgacgc ccctttcctg ctggtgacct ccgcatcaca tctgccgcgg
     7681 gcgatgatct tttttcagca ggaagggtta aatcctctac cagccccggc gaatcagttg
     7741 gccatcgact cgccgcttaa tccgtgggaa cgggcaatcc cttctccggt ctggctgatg
     7801 catagcgatc gtgttggcta tgaaacgctt ggccgcatct ggcagtggct gaaaggtccg
     7861 tcaggcgagc cacggcaaga gtgattttga tgccagatcg aagttgcgcc gactaaatcg
     7921 tccggtatta accagttgcg caacttcatc ccataacaca tacaaccaac gccgccagat
     7981 gaacgactcc gccactggcg cgcgttgcag gtaactccag agcaaatctt ccgccagaga
     8041 attatccatt agtcggaaca gttcgtattc tcgcggtgcc caaagcatta gtcccggccc
     8101 gaccatcgcc agtaactgat cgctgcgcga atctttcagc atgctgcgta aacagaagtt
     8161 accgtgaatc aacacacaat tgtcgttaaa gccttcaaat aatgccggga gacattcgcg
     8221 agtgcgaaac aggatccgct tatcctgcat cgtcagaccg gtgttattga actgattgag
     8281 cgtggtccat agcacttcaa catgttgccg gtaccatgag ggccagaaat gttcctgagt
     8341 attgtcgacc gcgccgacgc aaccgcgact gtcctgacgg tgccaggcca gtaaggcttc
     8401 aacgatttgg tctttgagct gttcccagcg ttctggtgtt cgggctggtg cctccaccga
     8461 aacaccacgc atccgctcca gtagcaggac atcagggccg gggtgttctt catgcgtcat
     8521 cacaccataa atagtcggca tacggacagt cccacttctt gccagcatgg tggttttcca
     8581 tgccagttgt cgggcttttc cgggcgtact aaagcttctt gccattaacg gcattgggtt
     8641 tccctggctg tcatacaaag cccacaacgc cgtatccgct ttttcattga cgcactcaat
     8701 acgactgagt ttttcgccca gtaaatggct taattcggca cgcagctgtt ccattcagat
     8761 taccctcata aaagctactg ctttcataat gagcgccaat ataaaagatg tcacctgaat
     8821 gagatcaatt gttgaaaaga aaaattgagg aaaatcgtaa cggtacgaca ctcctcccaa
     8881 aattggctga agtgtcgtga agtgaaatta gcgcatttca gcgcgaacgc gctcaagatc
     8941 ttcaggggta tccacacctg tgccaggaac ttcctgagca acagcaacat ggattttttc
     9001 accgtaccac agaacacgaa gctgctctaa catttcgatg tgttctaacg gacttgcctg
     9061 ccagttgacg taacgacgga taaagcctgc acggtagcca taaatgccaa gatgacgcag
     9121 gaagttatcg ccaacggttt caaggccttc tgcaaaacga tcacgatccc aaggaatggt
     9181 ggcgcgtgag aagtacagtg catacccttc agcgtcgaga accactttca ccgcattcgg
     9241 gttaaacgct tcttccgcat tgtggattgg caccgccaga gtcgccatac ccacctgacg
     9301 ctgagcgagg ttatcagcaa cctgacgaat gattgtcgca gggatcatcg gttcatcacc
     9361 ctgcacatta acgatcaccg tgtcgtcgct gaatgcgcat ttttcgacaa cttccgccag
     9421 acgttctgtt cctgactgat gatcggcgcg cgtcatacat acttcaccgc cagcggcttc
     9481 aacggcgcgg gcaacatcct catgatcggt tgccacgatg atgcgctcgg cacctgattc
     9541 acgcgcgcgt tcaagaacat gaacaatcat gggtttgccg ttaatatcaa ccaatggttt
     9601 accgggcaga cgcgtggacg cgtagcgcgc gggaataatg accacaaaac tcatgattta
     9661 ctctcatcag cagtcagcac gcgggcttcc gtttccagca acactggaat gccatcgcgg
     9721 agcgggaaag ccaggttatc cagtttgcaa ataagctctt gtttttcctg gttataccaa
     9781 agttttccgt tgcaaaccgg gcaggcaatg atttcaagca aacgatgatc catagttcct
     9841 ccagatggat cgggttatga atgcataaat cttatcataa tcatttatgc gacggggtct
     9901 atttcccagc cagtgcggca atcagtaaag agaccttgcg ggcagcgtcc taatgtcacc
     9961 cgcgttgcct gctgccagct cgcgaaatca gtaatcgcct gacgtaaccc tttttgcagc
    10021 attgtcgttg gtttaatgcc ttcctgtaac cacagagaga taacttcaag gatgcctgtc
    10081 tggcgatgca ttttggcatc cattcgccca actaattgcc cacgatgtaa taatggcaga
    10141 acaaaatagc catactggcg tttcggcgct ggggtatagc actccagccg gtagctaaaa
    10201 tcaaaaagct gctctgcgcg tttgcgatcc cagacaacag gatcaaaagg cgaaagtacc
    10261 gcgctgtgcg ttgcagtgag ctttccggcc agcgcacgct cgagtagcgg cagcaaatca
    10321 gcatgcagcc aaagattgcc caatttttca acatgcacag caatgatttg ctgctgttca
    10381 gccctcgctt cgcgccacgc cgccagtgcc ggacgtttca gccgatagta atctgccagc
    10441 cactgttcgc ggaatattcc caggctacgc gcactgttat ccagcatgat gatttctgct
    10501 tctgtttgcg aaacgagatc gcgctcatca tcccagtcag gcatgacacg gtgggttaaa
    10561 tcataaacgc gctggaagtt gcgccgttca atcaccatca cctttccggc agtaaataaa
    10621 ccttccagat gccgtttatg cggtttccat tcccaccagc cgcttgcacc tttacgagga
    10681 tgctcaaaat cggctgaacg taccggcccc ttatcatgaa tatgctgaat taactgtgca
    10741 atttccgcct catgttcctg catccaggcg tctttgtatt tccagcccat tttttcaagt
    10801 gccagcatgc ggtggcgaat aagacgaaag tcgctacgcg gcataaagca ggcttcatgc
    10861 gcccagtatt ccattaattc gccacgcgcc agagactcat ccagccactg ggcaggataa
    10921 tttcccagac gactgaaaag caccagatat ggactacggg caacaatatt gatggtatcg
    10981 atttgcagca aggacatgcg ggagatcgtt gccggaatat cctccaacga cgctcgacgg
    11041 cggggtttgt ttaacaggcc ttgtgcggca aggtgaagat tacgcgcatc agcaagggag
    11101 aggtgcggca gcgacattca tgactccatc aatcgaacgc tgccgcggcg taactagttg
    11161 ccagaagcca gcgaggttag ttgcgtaagc agtttcgctg gttcatcacc tgaaagctgt
    11221 gcgtctacag gcaaatacca ccaattttct tctgcaaagg cccggcattt caccgcatct
    11281 ttttcagtca ttaccagcgt ttgcccggcg cttaccaacg cactgacatc cgcatggttc
    11341 aaagactgat gatcggccag cggtacacat ttttccggtt gtacgccaca catcttcagc
    11401 gtggcaaaaa agcgcggcgg atgcccaatc cccgccatcg ccactacatg ttcaagctga
    11461 gcaacgtcac aacgcgtacc ggtacgtaaa ttcaccgcct gacccggcag cagatgcatg
    11521 ggaatttcac cgctgcgagg gacaccaccg ttgacgatta ccgcatcaac cgactttaag
    11581 cgccccgctc gctcacgcat tggccccgcc ggcaaccacc agccattgcc aaagcgacgc
    11641 acaccatcaa taacgacaat ttccacatca cgcgccagac ggtaatgctg taaaccgtcg
    11701 tcggttacga tgatctgcac atcagggtgt tgcgccagaa tggcttttac cgcatcagaa
    11761 cgaacgggag aaaccgcaac aggcgcatca gtgcgttgat aaatcaacac aggttcatca
    11821 cccgcctgtg ctgttgtggt atctgccgac aataacagcg gataagattc agccttacta
    11881 ccatatcccc gcgatacgac ccccacgcga ataccgcgct gttgcaactg ttccaccagc
    11941 cagacaacga ccggggtttt tccgttgccg cctgcggtga gattaccaac cacgacaacc
    12001 ggtacggggg cacgccaggc gcgcttcagt tttagtttat agcaaagacg gatcgcgcca
    12061 ctcaccaggc catacaacca ggagagtggc agcaatagcc gccacaaagg ggattcacca
    12121 gaccagattt tttcgatcat tggccaaact gcattttgtg aagttgcgcg taaacgccgc
    12181 ggtgctcaag caaatcgtta tgcgtaccgc gttccacaat gacaccatcc tcgacgacca
    12241 cgatttcgtc tgccttttca atggtagaca agcggtgggc aatcaccaga gaggtacggt
    12301 ttttctgcaa ctcatccagt gccgcctgaa tcgcacgttc ggattcggta tccagagccg
    12361 aggtagcttc atccagaatc agaatcgggc tatcacgcag caaggctcga gcgatagcaa
    12421 tacgctgacg ctgaccgcca gagagcagca cgccgttttc accaatcact gtatcgagac
    12481 cgttatccat cttattgatg aagtccatgg cgtaggccat acgcgccgct tcttcaattt
    12541 gctcacggct gtactgttca gtccgtgcgt aagcaatgtt gttagcaacc gtatcgttaa
    12601 acagatggac attctgcgac accagagcaa cctggttacg taacgacgcc agggtatact
    12661 cgcgcagatc gtgaccatcc atcaggattt cgccttcatc aatatcgtaa aaacgagtga
    12721 tcaggctggc gatggttgat ttacctgaac cagagcgtcc aaccagagca accgtcttcc
    12781 ctgccggaat tttcaggttg atgttacgca atgcaggtac gtcacgtccc ggataagtaa
    12841 aggtgacatt gcggaattcc acgtcgccag tcgcacgctc gatcacgcgc ttaccttcgt
    12901 ctttctcctg ctcactgtcc agaatggtaa acagcgtctg acaagccgcc ataccgcgct
    12961 ggaactgggc gttaacgttg gtcagtgatt tcagcggacg catcagtgca atcattgaag
    13021 agaaaacaac ggtaatcgta ccagcagtca ggctatccat gacacttggg aagctcgccg
    13081 catacagaac aaacgccagc gccaaagagg cgatcagctg aatgatcgga tcagagatgg
    13141 aagaggctga aaccattttc atcccctgaa gacgcattcg gttgctgact ttatcaaagc
    13201 gtttcgtttc cacttcctga ccaccgaaaa tcaatacttc tttgtggccc ttcagcattt
    13261 gttcggcgct ggtggtcacc tgccccatgg tgttctgcat gtttttactg atgttgcgaa
    13321 aacgcttcga tactacgcga atcgcaatcg aaacaatcgg tgccagcaca atcaaaatga
    13381 tcgacagttg ccaactgtaa tagaacatca tgatgaacag gccgatgatt gacgcacctt
    13441 cacgcacaac cgttatcagt gcgccggaag aagaagaagc aacctgttcg gaatcgtagg
    13501 taatacgtga caacagcgtc cccgttgact gtttgtcaaa aaatgaaacc ggcattccca
    13561 tcatgtgacc aaacaggcgg cgacgcatgg tcattaccac ctttcctgat acccaggaga
    13621 tacagtagct ggagacatag ctggtgatac cacgtaaaat catcagcccg atcaccacca
    13681 gcggcatcca caccagcacg gagcgatctg ttttaccaaa gccatcatca agaagtggct
    13741 taaggagcga taacatgaag gtatcgctgg ctgcgttgag gattaacgct acgcccgcca
    13801 cgatcagacc cgctttaaaa ggcgcaatgg ttggccacag tcggcggaac gtctgccacg
    13861 tagagagatc tttgtcgtta tgcattcaaa aaaccagcat ttgttgaaat agccgcatat
    13921 tctacccgtt atccactggc acgccaaacc actgatgata ccaacgaggt aaaatttgct
    13981 ctctgaggct gctaatccgc cagccttgcg ctgaaaaatt gaccgtcact tgaccctgat
    14041 gtggagtatc aagccattga tatccttgct gttgatagcg atgcttaact ttattagagg
    14101 gcaatcgcca tgcgttatag cgttatagcg cgatgccgat gcgagtgcca cttttccatt
    14161 cactcgctga attaatggca acgatgatga ggtattactg ccatggtgag gtacctgaag
    14221 caatgttgtc tgcacttgct gccagtaacg gcttagcatc ttttgttcag cggggacttc
    14281 aatatcaccg gttagaagaa tgctattcgt cccgtcataa accttaacca cacaggaatg
    14341 gttatttcct ttatcgttgc tagcttgtaa aggccagtgc acgctgaaac gcaatccttg
    14401 ccattgccac gcttcgccac gcacacaggg ctgatgatgt tcccagttta acggacttct
    14461 gatccataac atcggccatg tgtgcaatat tgagtccagc cctccccggt gatccagatg
    14521 ttcatgactc agaataacgc cttccggttc aagattatgc cagtggagcc aggggataat
    14581 cagttgttgc ccactatccc cttcgggcca ggccagtcct gtgtcataga gaatcgcttt
    14641 gccgtttctg gctatcacca ttgccagccc ttgcccgaca tcaagcatgt acacctgcca
    14701 ctcgtcaggt cgaggttttt gccacagcgg ccagcacatc agcaagccta cagccacaca
    14761 cattgctggc aacgttcgcc aggcgtttaa tcgccatacc accagtaaga accatgggga
    14821 aaatgatagc cattgccaac gttcagcaat gttgatccac ccttctggca aactctttaa
    14881 cccccagaaa agtaaagcca aagaccggtc ggcaagaaac cataaccctt gctccaggat
    14941 taacggcccg cttaaatgca caaccatcgc ggcgaggatc aacggaaccg tgataaatgt
    15001 caccaaggga attgctaata gatttgcaat aaacgaggtc agactaatgc catgaaatat
    15061 gacgatttgc acaggcataa gcaggagtgt gattcccagt tgcaaatgaa tgagggaaac
    15121 aactgcacgc aataccggcg gcagttgcca ctcaggacag ggaaaccact ggtaccaaaa
    15181 aatcagtgcc gcgaccgcag cggcagagag ccataaactt tgcgagagaa tggcaacagg
    15241 atccatcagc aaaattgccg ccagacaaca tatccataca tcccagccac tccactgtcg
    15301 cccacttaac ttaagcattc cccacgtagc aagcgccacc acggtacgca atgcaggagg
    15361 ttgcattccc gtcaaccagg cataaaaagc agcacagcag attccaccaa ttaatggcat
    15421 ttgccagtgg atccagcgcc caggcagaaa aacttgccca ccgcgaatga gtccggcagc
    15481 cagcaacgcc gcaaaagcga tgtgcaatcc cgatatcgcc attaaatgcg ccgttccagt
    15541 atcacgcatg atatttttga tttctttggg gacggataac cgttccccca tacctaaacc
    15601 aagaataacc gcattccaca tatagggttg cagcgttgtt tgtagtgacg ccagatactg
    15661 tgcacgcagg ctacaattcg gttcaatgac acttgcctgc agaaagcggc cgatgagcgg
    15721 ctgatgctgg gcaatggcat aacgctggct atcaaagccg ccatcattaa gttggccatg
    15781 aactgcacga actttgagtt tcattgacca ttgttgtcca gcacaaaccg cttgcggaag
    15841 atattcgcca tacagcacaa gacctggcgc agggaatata cgtcgacctt gtagatgagt
    15901 aatttgaccg taatgagtgg tcatgccgtc agttgcagtg atctcaacaa ttgcatcctg
    15961 cgtcgcgcca gttaaggttt ctcctgccca caaaatttgc tttgctgata atatgcccca
    16021 aacgaaaaac agcaacgtca gcgcgatata acggacggtt ttaacaggga taaatgccag
    16081 tacgcaggcg aagagagtca gaaacgcaag ggttaatgtc ccaggcaatt ggggcaaaat
    16141 cagcaacgga aaaattccgc aaattatgca tacgccgact gtcgttattt tcatgcaccc
    16201 ctccctgttt atggaaagtg tgcaactttg ttgagcggtt gtctggtggg aattttgcgt
    16261 tccccgaggc acattcagaa gaatttaacg aaagggtgaa aactgctcaa aactctgcga
    16321 ggcagcttcc agttcctgat tagagaaatg aaaaaagcac ccgacaggtg cttttctcgc
    16381 gttcaagttt gagtaaaaac ttaaccgtaa atattggcgc gatcgcgcag ttctttacca
    16441 ggtttaaagt gaggaacgta ttttccttcc agttctactt tatcgccagt cttcggatta
    16501 cgtccggtac gtggtgcgcg gtagtgcaaa gagaaactgc cgaaaccgcg gatttcaata
    16561 cgctcgccct gcgcaagagt cgaggccata tgctccagca tctcttttac tgcatcttca
    16621 accgtcttgg cgggaatgtg cgattgctgg gtggcaagtc tttctatcaa ttctgacttg
    16681 gtcatgattc ctccggttcc ttaaaggcaa attaagcggc tgctgttgct gcattgatta
    16741 agggcggccg tagccgccct tagtgcttga ttacaggacg aaacctgcaa tctgtcaagt
    16801 aaactcaaca aacttcggaa taaaaatccc gaagagtcag agaattactc gcctttagct
    16861 gctttgaaag cttcagccat tgcgttgttg gagaagtttg catcttcctg tttgttaaca
    16921 gttgcgattg catctttctc gtcagcttcg tctttcgcac gaacagacag gctgattgcg
    16981 cggtttttac gatcaacgcc ggtgaattta gcttcaactt cgtcgccaac gctcagaacc
    17041 agggtagcgt cttcaacgcg gtcacgggat gcttcagaag cacgcaggta accttcaacg
    17101 ccgtcagcca gttctacggt tgcgccttta gcgtcaactg cagttacttt accggttacg
    17161 atagcgcctt tcttgttcag agcaacccag ttgttgaacg gatcttctgc gagctgttta
    17221 acgcccaagg agatacgttc acgttctgcg tcaacctgca gaacaactgc agcgatttcg
    17281 tcgccttttt tgtattcacg aactgcttct tcgcctgcaa cgttccagga gatgtcagac
    17341 aggtgaacca ggccgtcgat gccgccgtct aggccgatga agataccgaa gtcagtgata
    17401 gacttgattt taccttcaac acggtcgccc ttgttgtggg tttccgcgaa ctgctgccac
    17461 gggttagctt tgcactgttt cagacccagg gagatacgac gacgttcttc gtcgatatcc
    17521 agaaccataa cttccactac atcgccaacg ttaacaactt tggacgggtg gatgtttttg
    17581 ttggtccaat ccatttcgga aacgtgtacc aggccttcaa cgccttcttc gatttcaacg
    17641 aagcagccgt agtcggtcag gttggtcacg cgaccagtca gtttggtacc ttccggataa
    17701 cgtttagcga tagctaccca cggatcttcg cccaactgtt tcaggcccag ggatacacgg
    17761 gtacgttcgc ggtcgaactt cagcacttta acagtgattt cgtcgcccac gttgacgatt
    17821 tcgctcggat gcttaacgcg tttccaggcc atgtcagtga tgtgcagcag gccgtcaacg
    17881 ccgcccagat caacgaatgc accgtagtca gtgaggttct taacgatacc tttaacttcc
    17941 atgccttcct gcaggttttc cagcagctga tcgcgctctg cgctgttttc ggattcgata
    18001 acggcacgac gagaaacaac aacgttgttg cgcttctgat ccagcttgat tactttaaat
    18061 tcaagctctt tgccttccag gtgcagagtg tcacgcaccg gacgaacgtc taccagagaa
    18121 cctggcagga acgcacgaat accgttcagc tcaacagtga agccgccctt aactttgccg
    18181 ttgataacac cggtaacagt ttcagcatct tcgtaagctt tttccagcgt gatccaggct
    18241 tcgtgacgtt tagctttctc acgggacagc agagtttcac cgaagccgtc ttctactgcg
    18301 tccagagcaa cgtcaacttc gtcacctacc tggatttcca gctcgccctg ggcgtttttg
    18361 aactgctcag ccgggatggc ggactcagat ttcagaccag cgtcaaccag tactacgtct
    18421 ttgtcgatag caacaacaac gccacgaacg atagaacccg ggcgggtttc gatttctttt
    18481 aaggactctt caaagagttg agcaaaagat tcagtcatgt ttaatcttca ggtttatatt
    18541 taacgtccac ctggctccat gccggatggg gttgtttaac atacccgctg tcattccatt
    18601 gcaacggggg tactgcaaat tcggtcgctt atgcgagagc caatttctgg cgcgcgtatt
    18661 gtagcgcttt ttcaatcact tgctcaatgc ttaaggtggt ggaatccaac actaaagcat
    18721 cggctgccgg aaccagtggc gctaccgctc ggttacgatc gcggtcgtcg cgttctttga
    18781 tctcggccaa aaggcgctca aagttaacac taaagccctt ctcctgcaac tgtagcatgc
    18841 ggcgatgcgc acgttcttcc gaggaggcgt caaggaaaat tttcactggt gcatcaggga
    18901 ataccaccgt tcccatgtcg cggccatcgg caatcagacc tggtaattcg cgaaacgcgc
    18961 gttggcgacg caataatgct tcacgaacgc gtgggaatgt cgcgacttgt gaagccgcat
    19021 tcgccacttc ctgagtacga atttcgccgc tgacatcttc cccttcgagg atcacttcca
    19081 gattgccatt ggttgacaca aaacgtacat ccagatggga tgccagcggt accagcgcat
    19141 cttctgacgc aacatcaaca tggtgatgta atgccgccaa tgccagtacg cgataaattg
    19201 cacccgaatc cagcagatgc cattgcaatg cttccgccat agccttacac aaggtgcctt
    19261 tccctgcacc gcttgggcca tcaatggtaa taaccggggc aattgccgtc atctttatct
    19321 ccttaaaaca ggcgtaccgt taacataacc gcgcgcatta tacgcgccga cgccacaaat
    19381 cgttaacgtt gtgtgcaaaa accagttttg tctgtattca gatggggatt tattgcgcaa
    19441 ttatagcggg ctggcagaag accagcccga aagatgacaa tgatttactt accagaggcg
    19501 atacgatcac ggatgtgttg cgcacgctct gttgatggtg ggtgagagtc aaacatggac
    19561 tgggtgcgac cgccatccag actagccagt ttttcaaagc tgccaaccag cccctaggtg
    19621 ctgatgccac gtttcttcaa cagatcaaag gagaaatcat ctgcttcgga ctctttatca
    19681 cgggagtatt tagcattaat agcgccttct gcgatatcac ccaattgtga gcgggaaagc
    19741 tgggaagcca caccgctggt agctgaaatg gcatcgcgtg cggcaacgat cgcataagaa
    19801 actttcattt cagccagcga gtgacccaac gcgacgtggc ccagttcatg gcccagaaca
    19861 ccttcaattt cgttgtcatt catcatgtcc atcaggccac tgtagacacg gacacagccg
    19921 ttcgccatcg cccatgcgtt gacgtcgctg gtcatataaa cctatagttg accggcgtgc
    19981 cattaatatt gttacccaat gctttggcga tttttgccag acgtttaccg tacttgctgg
    20041 atttacttgc gacttgattg ccgctgtcca tttcggcaca gccctgattg gcaatcgctt
    20101 ttgcatcggc atcgcttaat gttgctgctt tataggcatt taaaccggag ctgatagcca
    20161 tattggtatc aataccgtgg gtattttgac accctgtcag taatgctgca gaggtcgcaa
    20221 tcgccagcag taatttagta ttcttcatgt gaatcatccg ttgattaatc atatataaac
    20281 attatgtatc aacaacttac attgacaagc gacgctacca ttaatcaaaa tgactcacaa
    20341 ggtccgaaaa aaaacgccga caatgtgc
//
LOCUS       DAJTNN010000064        20201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTNN010000064 DAJTNN010000000
VERSION     DAJTNN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.064"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(42..1064)
                     /locus_tag="OO150_002023"
                     /pseudo
     CDS             complement(42..1064)
                     /locus_tag="OO150_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(1074..1886)
                     /gene="hcaB"
                     /locus_tag="OO150_002024"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(1074..1886)
                     /gene="hcaB"
                     /locus_tag="OO150_002024"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5516177.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(1883..2203)
                     /locus_tag="OO150_002025"
     CDS             complement(1883..2203)
                     /locus_tag="OO150_002025"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5516178.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGSDIFIDLP
                     EAQP"
     gene            complement(2203..2721)
                     /locus_tag="OO150_002026"
     CDS             complement(2203..2721)
                     /locus_tag="OO150_002026"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5516179.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRPRHLISNC
                     QISETDIPNVFAVRVNYLLYQAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(2718..4079)
                     /locus_tag="OO150_002027"
     CDS             complement(2718..4079)
                     /locus_tag="OO150_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5516180.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            4215..5105
                     /gene="hcaR"
                     /locus_tag="OO150_002028"
     CDS             4215..5105
                     /gene="hcaR"
                     /locus_tag="OO150_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5516181.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKTVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            5265..6404
                     /locus_tag="OO150_002029"
     CDS             5265..6404
                     /locus_tag="OO150_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5516182.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(6396..7676)
                     /gene="csiE"
                     /locus_tag="OO150_002030"
     CDS             complement(6396..7676)
                     /gene="csiE"
                     /locus_tag="OO150_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5516183.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(7867..8721)
                     /locus_tag="OO150_002031"
     CDS             complement(7867..8721)
                     /locus_tag="OO150_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5516184.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(8866..9669)
                     /gene="suhB"
                     /locus_tag="OO150_002032"
     CDS             complement(8866..9669)
                     /gene="suhB"
                     /locus_tag="OO150_002032"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5516185.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAVEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            9788..10528
                     /gene="trmJ"
                     /locus_tag="OO150_002033"
     CDS             9788..10528
                     /gene="trmJ"
                     /locus_tag="OO150_002033"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5516186.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10798..11286
                     /gene="iscR"
                     /locus_tag="OO150_002034"
     CDS             10798..11286
                     /gene="iscR"
                     /locus_tag="OO150_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5516187.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            11398..12612
                     /gene="iscS"
                     /locus_tag="OO150_002035"
     CDS             11398..12612
                     /gene="iscS"
                     /locus_tag="OO150_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5516188.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            12640..13026
                     /gene="iscU"
                     /locus_tag="OO150_002036"
     CDS             12640..13026
                     /gene="iscU"
                     /locus_tag="OO150_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5516189.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            13043..13366
                     /gene="iscA"
                     /locus_tag="OO150_002037"
     CDS             13043..13366
                     /gene="iscA"
                     /locus_tag="OO150_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5516190.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            13462..13977
                     /gene="hscB"
                     /locus_tag="OO150_002038"
     CDS             13462..13977
                     /gene="hscB"
                     /locus_tag="OO150_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5516191.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            13994..15844
                     /gene="hscA"
                     /locus_tag="OO150_002039"
     CDS             13994..15844
                     /gene="hscA"
                     /locus_tag="OO150_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5516192.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            15846..16181
                     /gene="fdx"
                     /locus_tag="OO150_002040"
     CDS             15846..16181
                     /gene="fdx"
                     /locus_tag="OO150_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5516193.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            16193..16393
                     /gene="iscX"
                     /locus_tag="OO150_002041"
     CDS             16193..16393
                     /gene="iscX"
                     /locus_tag="OO150_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5516194.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            16571..17854
                     /gene="pepB"
                     /locus_tag="OO150_002042"
     CDS             16571..17854
                     /gene="pepB"
                     /locus_tag="OO150_002042"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5516195.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            17996..18772
                     /gene="sseB"
                     /locus_tag="OO150_002043"
     CDS             17996..18772
                     /gene="sseB"
                     /locus_tag="OO150_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5516196.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(19265..20110)
                     /gene="sseA"
                     /locus_tag="OO150_002044"
     CDS             complement(19265..20110)
                     /gene="sseA"
                     /locus_tag="OO150_002044"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5516197.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPKTFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
BASE COUNT         4811 a         5149 c         5503 g         4738 t
ORIGIN      
        1 ctatttcagg aaggagtgtc cgttaaactc aggctacctc agtcatcgcc acgcatatcg
       61 ccaataaact gtaagttatc actgtactga tcgctccaga accacggcag tagcggtagc
      121 ggtagcccta acattgcggc agcggcaatt tgcgcctggt tattggcgtt ttcccagctt
      181 tcacagcggt gtagtgcacc attatcaaga cgagtgattg ccacatcgcc accggcaaag
      241 atcgcgggat cgcaggtgcg gcaagcctca tcaatgacaa tgccattggc agtatcaagg
      301 ttggcctcgc gagccagttg ctcgttggcg ctgataccaa taccgtaaat caccacatca
      361 gcctgaagcg tttccccact ttgcagcgtc agttctactt tttcaccatc gaccacatgt
      421 tcaatggcat tattgagcag aatgcgcaca ccagcctgct ggtggcgctg taaaagatag
      481 cgttgcacgg gcggtggtgc attacggccc atgacggttg ccgccagttc aatcactgtc
      541 accttacatc tgcgctgcgc tgcgctgcgc tgcgtggcgc tggcagccag ttccagacca
      601 atagttccgg caccgacaat cacgactgac cgttcgggct gcagaacttc tcgcagtctg
      661 gcggcatcac cggcatggcg tagggtaaag cagcgttctc ccagtgcatc aagcaacggc
      721 agcggtcgag ctgccgcgcc ggttgctata aaaagctgat cccagtgcca gctttcgccg
      781 ttggttaaca ctaactctcg tgtgtcgcga cccagcgttt tgatggttac accggaatgc
      841 agatgaacat tgttttcctg ccaccagtta gcgggtaaca cctgctgtaa ctgcggggaa
      901 tcttccagca acatggattt cgagagtgga ggtcgttcat aaggaagatg tcgctcatcg
      961 gaaaacagat gcagctcacc ggtgaaccct tgctggcgta gcgaggccgc agccattgcc
     1021 gccgcttgca cgccaccgac aataatgatc gttttttctt tcatcgtgct tccttaaaga
     1081 tccagcccag ccgctacgtg gcgaatgccg cgaatcgcta aacccgcatc agcgttgatc
     1141 atcacaccgc ttaatgcgcg attattgcgc cgcgatgcca acatcacata cggccccgta
     1201 aaatccgccg gttgcgggaa aaattgcagc ggtaaaatgg cggcaatttt ctccggcgtc
     1261 agagactgca ttatcgaggt ttcactttgc ccgagcgcct gtgggccgcg caggtcgctg
     1321 gccataccac acgggccgac gccattcacc cgcactttcg gtgccagttc ataagccagt
     1381 tggcgaataa gtccggttgc ggcatgttta ctggcggtgt acagcgggcc accgccgcca
     1441 ggataccagg cggcatttga cagtgtgaaa atcatgctgc cttcactggc gattaacgcc
     1501 ggagcgcagg cttttgcgcc cagcaggtaa ccgagtacgt tgacgttaaa cagctcgtgg
     1561 aagccggttt cgagcgtctc tgcgggagta ttaaccagtg aggcattgtg atcccagatg
     1621 cctgcattgc cgataaaaca atccagcttg ccggaacgag tcaggatctg atcgaccgcg
     1681 cgttgataat cggcataaca ggtcacgtta ccttccaccg ccagaatatg ttcgccaaat
     1741 cgctgacgca gactggcgac ttttgccgcc gacagttcca gcgtggcaac ctgcgcgcct
     1801 tcttcgataa atcgctcgac cagcgccagc cctaatcccg atccgccgcc ggtaataaaa
     1861 atggactcgt tatgcagatc gctcatggct gcgcctccgg taagtcgatg aaaatgtcac
     1921 taccttcaac gtgtactgga taagtggtga gcggatcggt ggcgggcagg cataacgctt
     1981 tccccgtttt caggcaaaaa ctggcggcgt gtagcgggca ctccaccgtg gcgtcatctt
     2041 ccagataccc ttctgacatc gacgcattac catggctgca acgatcgtta atggcataaa
     2101 actcgccgcc aacgttgaac agggcgatga cgggcgaggt atcgatccgg agagcctcac
     2161 cctccggcac atccgcaacg ggacacgcat aaattcgatt catcagaaca gtacactcag
     2221 gttatgggaa gtgattaccg cttgatccag gacgatatcc cgttccagca agcgccagtt
     2281 gtcatcttcc agacggcgaa ctttgtcgaa acgcgttcca acatagaatg tttcatcgcg
     2341 ctctttttgt gcctgataaa gcagataatt tacccgcaca gcaaatacgt ttgggatgtc
     2401 ggtttcgctt atctggcagt tgctgattaa gtgacggggg cgtgacggcg gctcttctgc
     2461 ccaggccatg cccgtttcca gacgggcgat tcgccgctcc agctggtctt tggtgtcatt
     2521 aaaaatccag gttgtcggtg gctggacgcc tttgcggcgg tcgcgtgttt gcgcgttaac
     2581 tgtggtgcgc atggtgtaac gaatctcttc gtcgagctgc gccagccagt cacgaaattt
     2641 ccagtcgtcc agtaagctgg cttcgtgaaa gagaaactgg ctaatgcgga gatgtaactc
     2701 tagtgaaact tgcgcactca tttcatcacc tcctgctggt aagcggcggt tttatcgagc
     2761 acttcctgcc agctttcgct actcagaaga tcggcccagc gttggtacat tccacgagcg
     2821 gcagtttctg aaaagatata gttagtaatg ccaggaatgc cgtcgtcgcg gcgcttttcc
     2881 tgaccaagcc ccatttccag acacagtttg ctgttgcggg cgcggtggcc tttaagcaat
     2941 ttctggattt cacaccagtt ctccgagtca tcctgctcga gaaaaccagc aggaccaaaa
     3001 gcacgagtgg cgctgttttc aaaagcggct ttaacttcat cggaggcggc tttgtcagta
     3061 atacagaacg cccacacttc aacttgatca gggccgcgcg gatgccagac gcggagtgtg
     3121 gcagtgccgt ttagccatga aagcgtgggg aatatattgt tatgacccgc caggcgcagg
     3181 gcgcgaactt cacctaaacg ttgttctgct tcggcatagg tttcgcggta atagctggaa
     3241 accgcgccat cgacccagac attagcatcc ggtttttcag taaagaagaa accgctaccg
     3301 tgaccgtcct gaccaaattg cagcgcatct ttggcggttt cccacaccgg gcgggcggtt
     3361 tgtccatcac cgagacgctt atcgctgcca tcatctttcg cccctaatac ctgaacggca
     3421 gaagcatggc tgaacagagc atgatattgg tcactggcga actgctctgc cggggatttc
     3481 cagttacagt tgatcaccca cttttgtacg ccgccgacaa tttcggtgcc gccttcgcga
     3541 cgatccagca tgccatccag ataccaggca atgtcaccaa ggtaatcacg caggcccggt
     3601 gcgctggtat cccagttgcc aaaaatcagc cctttataac tctccacaca aggaacttcg
     3661 tttagccccc agtgggattt acacaaccct tgtgggtagg cgcgaggttc cagcggtaca
     3721 tcgatcaact cgccgttaat gccataagac cagccgtgat acgggcaggt aaaggcgcga
     3781 gtgttgccgc aatctgcata actcacacgc atggcccggt ggcggcattg gttgagaaac
     3841 gccttgatgc tgccgtcttt ctgacgcact acgacaaccg catcttctcc catgtaggtg
     3901 ttaaagaaat caccgggttt tgggatctgg ctttcgtggg cgagaaatag ccagcaacga
     3961 ccgaaaatac gctcaagctc cagttggtaa atatccgggt cggtataaat acgcggagtg
     4021 acccgaccat tttgggtgtc aatcagttgg taaatgttca aatctgaggg tgtggtcata
     4081 ttttttaatc ctgaaagata agatgtattg accttctatt tttgtgccat aaggatgttg
     4141 ctggttggtt gctaatatgt gaaatagtta atttctacca gctgagacgt tttttcgata
     4201 acaaggggaa ggtgatggaa ctacggcatt tacgctattt cgtcgcagtg gcgcaggcac
     4261 tgaactttac ccgtgcggcg gaaaaactgc atacctcaca gccttcgtta agcagccaga
     4321 tccgcgatct tgaaaactgt gtcggtgttc cgttactggt gagggataag cgcaaagtcg
     4381 cgttgacggc ggcgggagaa tgttttctcc aggacgcgct ggcaatcctc gaacaagcgg
     4441 aaaatgccaa attacgggcg cggaaaactg ttcaggaaga cagacaatta accattggct
     4501 ttgtgccatc ggcggaagtg aatttactgc caaaagtatt accgatgttt cgtctcagac
     4561 agccagacac cttgattgag ctggtgagtt taatcaccac gcaacaggag gaaaaaattc
     4621 gccgtggtga actcgacgtc ggcttaatgc gccatcctgt ttatagcccg gagattgatt
     4681 atctggagct ttttgacgaa ccattagtgg ttgtgttacc ggttgatcac cctttagcac
     4741 atgaaaaaga gatcaccgcc gcgcaactgg atggcgtgaa tttcgtcagt accgatccgg
     4801 cgtattccgg ttcgcttgcg ccgatcgtta aagcgtggtt tgcgcaagaa aacagccagc
     4861 caaatatcgt ccaggtggca acgaatattc tggtgaccat gaatctggtg ggcatggggc
     4921 tgggcgtcac tttgataccc ggttatatga ataattttaa taccggacag gttgtttttc
     4981 gtcccattgc cggaaatgtg ccttccattg ctttactgat ggcgtggaag aaaggggaga
     5041 tgaagccggc attacgcgat ttcatcgcca ttgtgcagga acgtttggca agcgtaacgg
     5101 cataattacg cattattttt gcaacatgcg tcgcggatcg cagtttttta gtgcttgtga
     5161 actggcgtta cagacatcgg gaagcaggct cgaatattct tatttttcaa cttgcaccaa
     5221 cattcgatta accccagact aacgcctcct gacgggaggg actcatggtt ttgcaatcca
     5281 cgcgctggtt ggcgctcggc tatttcacat acttttttag ttacggcatt tttctacctt
     5341 tctggagcgt ctggcttaaa gggattggtt taacgccaga aaccatcggc ctgttattgg
     5401 gggcaggtct ggttgcccgt tttctcggga gtttgctcat cgcgccccgc gtcagcgatc
     5461 cttctcgcct gatttccgcc ttgcgcgtgc tggcaatgat gacacttctc tttgctgtcg
     5521 ccttctgggc gggggcgcac gtagcgtggc tgatgctggt gatgattggc tttaacctct
     5581 ttttctcacc gctggtaccg ttgaccgatg cactggcgaa tacgtggcaa aagcagttcc
     5641 cgcttgatta cggcaaagtg cgactgtggg gctcggtggc gtttgtcatt ggctcggcgc
     5701 tgacgggcaa actggtcact atgtttgatt atcaggtgat cctcgcgctg ttaacattgg
     5761 gcgtggcatc catgctgctc ggctttctca tccgtccgac gattcagcca caaggggcaa
     5821 gccgccagca ggagagcacc ggttggtcag cgtggttggc gctggttctt cagaactggc
     5881 gctttctggc ctgcgtttgt ttattgcagg gggcacatgc ggcctattac ggttttagcg
     5941 ccatttactg gcaggcagct ggctactcgg cctcggcggt ggggtatttg tggtcgctgg
     6001 gcgtggtggc ggaagtcatt atctttgcgc tgagtaataa acttttccgc cgttgtagtg
     6061 cacgcgatat gctgttgatc tcggcgattt gcggcgtagt gcgctggggc attatgggag
     6121 caactacggc gttgccgtgg ttgatagtgg tgcaaattct gcattgcggc accttcacgg
     6181 tctgccacct ggccgccatg cgctatattg ccgctcgcca gggtagcgaa gtcatccgtt
     6241 tacaggcggt ttactctgcc gtcgcgatgg gcggcagtat agctatcatg accgttttcg
     6301 ccggtttcct gtatcaatat ctgggccacg gcgtgttctg ggtaatggcg ctggtggcgc
     6361 ttccggcaat gtttttgcgc ccgaaagttg ttccctcatg ctgattcgag cattttgcgg
     6421 atctgctcct gttgatgtgt tgtcagtgtc aggtccgtat agatcagcgg tggtgaaaag
     6481 agcggtaacg gcatggtata aggcgcaata atcagtgccg cgccgcgcgg agcgcctgtc
     6541 tgcaaaaatg cctttaccga catatgttta atgttgagcg gcagtaacgt tagttcgcgt
     6601 aactgctgct caatctgcgc ttctcgctcg ctatcattcc cggtaagaag aataatctgt
     6661 ttttcatgca gatcgttttc ctgcattagc caggcaccga aaatcaccgc gaccagacca
     6721 ctttcctcat cagaaagatg gacgccgtat tcactttcaa atccggccag cgccgcgcgg
     6781 gtggtgcgca ccaggcgtgg gtacagtctg gcgaactctt ctggcagggt attatcaata
     6841 ccgatggcaa acaaactgcg atttaacgcc tgggcgaggt gggtataaag ctgatcgcat
     6901 aacccctgtt catcataaaa acgaacattt cccagctcac gaaaatggtt taccagacgt
     6961 ttgatagact gacgcaattg tctgtctcgc tgatgcgcat cacgcaatgg atcgggaacc
     7021 cgcaacatgg aaaaaagtaa tgccataaac agtggctcat caggcggtac aggctggagc
     7081 gcccgacgct gccagtggcg tccaatttcc tgcgctacct gaaattcaag gcaggattcc
     7141 gcccagcgac gttggagcgg attaaactga ggcgtaattc cagcgtggtg ttgcagcaaa
     7201 caatattgca gatacagaca caggaaatga atatcgcgcg tttcaaagcg tttttgcagc
     7261 cgtcgggagc agagattcac taacgcttgt agattggtat cgtcgtaaaa gttacgcgca
     7321 attccgcgcc gcttcagttc actcttcagg gcgggggtga attggctggt aataaatgac
     7381 ggacacagac ggaaaccacg tcgtagccag tgaaataaac acaaacgttg gttaagcact
     7441 gtaccttcaa cccggtagct accgtcataa ccagtcgtca gtgtgagttg atgatagcgc
     7501 aggatctccc gtcctgtttc gctgatatca agactggcaa tatcatcatc cacaccatta
     7561 agctcgctga aggttgccat ggtggcggtc aaccccggct gaaagagcgt cagcaagatc
     7621 tggcagcggc gctggggagc cgaaaggaca gatggtggag caagcgtagg catcatctgt
     7681 agatctccca taagcataat tgataagaat agctaaagtt tgctcaggta aatggcgaag
     7741 ggaatgctaa tcatcagaaa tgttggcaag cgtcacagaa tttttctcat cttaaactgt
     7801 tctgaccagt ggattagcat gtcttaaggt ggttttgtga atgattatcg tgctagcgat
     7861 catgggttag ttctgcggat tcaacgcgct aaggataaag tccaccatct gttcgcgata
     7921 aacatcgccg tgacgatcgg aaaaagcatc aatgtgttcg ccatccggga ttaggatcaa
     7981 ccgttttgtc tcttttgcca ggctatacaa cttttcgctg tgctgccatg ggataacgtg
     8041 atcagcttta ccgtgaatga gtaaaagcgg gatcgggctg acgctggcga tataattttc
     8101 gccgctgtaa ctctcatcaa gtaagtagcc actgccgggg atcatctgat tggcgatggt
     8161 tgcataagag gcaaatgtgg agtcgaggat caccgcacgt atgccttcac gatcatcctg
     8221 accaataaca gcaagaatat tcgccccgcc aatactctgc ccgaacagca ccagccgttg
     8281 tgggtttaca tcactgcgat ggcgcaccac attgatggca ctttgcgtat cgtccagcaa
     8341 tccggcctgg gacggcgtgc cttttgattt accaaacccg cgataatcaa acataaaaac
     8401 gttgaaatta cgctcgggta accaactgac cagcggccag tgggcggaca tatttccggc
     8461 attgccgtga gcatgaatga tggttgcgat ggcattgtca gcagggcccg tcgaagaagg
     8521 aataaaccag ccttgcagac gagtaccgtc cttagccgta aattcgacgg attccgccga
     8581 ccaggggtcg ggaccgtaaa ttttatcgtc aggataatag aagaagttga tggcaacgcg
     8641 cggcactaaa taaacgcaga aggcaacaac aaagagaata aacagaattt tgagaacgcg
     8701 tttgttcact ggcagcgcca taacctttcc ttgtgacttc tttatcatcc attaatcggg
     8761 gaaagatggt gggagagtga cgaaagaaag taaagcggtg aggaagtatt tcggaatagc
     8821 gcctgaaagg cgagggcggg tgagtgacat cacccgcctg agtcattaac gcttcagagc
     8881 gtcgcttaac tcatcacgca tgttcgccag catggctttc acaacacgcg ggttaccagc
     8941 aacgatgtta ccggtcagca tatagttatg accaccggtg aagtcgctga cgatgccgcc
     9001 cgcttcacga accagcagct cgcctgccgc gaaatcccac ggacgcagac cgatttcaaa
     9061 gaaaccgtca acacgacccg cagcgacgta agccagatcc agcgccgcag aaccggtacg
     9121 acggaagtct gcacattcgt tgaacagttt gccgacgatg ttgatgtagg tagtggcgta
     9181 ctgttttgct ttgaacggga agccggtcgc cagaatggta ccgtcgagat cgcgagcggt
     9241 gctaccgcgc agacggtagc cgttcagctg tgcgccctga ccgcgagtgg cggtgaacag
     9301 ttcgttacgc ataggatcgt ataccacagc aacttcggtg cggcctttga tacgaacggc
     9361 gatagatacc gcgaagtgcg gcagacgttt gataaagttg gtagtaccat ccagtggatc
     9421 gataacccat tgaacatcct gatcagtacc ttcaagttca ccgctttctt cggtgatgat
     9481 ggtgtgctgt gggtaagatt tacgaatcgt gtcgataatc accgcttcga cagctttatc
     9541 tacgttggtc acgaaatcgt tactgccttt ctggctcgct tctacagcgt ccggggtttc
     9601 atagtttttg gcaattaaat tacccgcctt gcgcgctgcg cgcacggcga tgttcagcat
     9661 cggatgcatc ggtctctctc actggatgtt aaagaacggg aaaacggcgc agagtatagc
     9721 agcgagatcg gcatctgtct tcgttttatg ataagatgcg tgaataatct ttagatgaag
     9781 aaagacaatg ctgcaaaata ttcgaattgt gctggtggag acgtcacaca ccggcaatat
     9841 gggttctgtt gcccgtgcca tgaaaacaat gggattaacc aatctgtggc tggttaatcc
     9901 actggtgaaa cccgactccc aggcgattgc cctggcagca ggggccagcg atgtgattgg
     9961 taatgctcac atcgtcgata cgctcgacga agcgttagcc ggttgtagct tggtggtggg
    10021 caccagcgca cgttcccgca cgctgccatg gccgatgctc gacccgcgcg aatgcggcct
    10081 gaaaagcgtc gctgaagcgg caaatacccc ggtggcgctg gtgtttggtc gcgagcgcgt
    10141 cggcttgacc aatgaagagt tgcagaaatg ccattatcat gtcgcgattg cggctaaccc
    10201 ggaatacagc tcgctgaacc tggcgatggc ggttcaggtt attgcctatg aagtgcgcat
    10261 ggcctggctg gcaactcagg aaaacggcga gcaggtcgag catgaagaga cgccgtatcc
    10321 gctggtcgat gatctggagc gtttttatgg tcatctggaa caaacgctgc tggcaaccgg
    10381 ttttatccgt gaaaaccatc cggggcaggt gatgaataaa ttgcgccgtc tgtttacccg
    10441 tgcgcgcccg gaaagccagg aattgaatat cctgcgcggg attctggctt ctattgagca
    10501 gcagaataaa ggtaacaagg ccgaataaca gtagttgcct gatgcgacgc ttaacgcgtc
    10561 ttatcaggcc tacaatgtac agaaccgtag gtcggataag gtgttcacac cgcatccgac
    10621 aacaggtaca aacgccacga taaaaaaatg gcactgaagg ttaaataccc gactaaatca
    10681 gtcaagtaaa tagttgacca atttactcgg gaatgtcaga cttgaccctg ctattcaata
    10741 cccccacttt tacaataaaa aaccccgggc aggggcgagt ttgaggtgaa gtaagacatg
    10801 agactgacat ctaaagggcg ctatgccgtg accgcaatgc ttgacgttgc gctcaactct
    10861 gaagcgggcc cggtaccgtt ggctgatatt tccgaacgtc agggcatttc cctttcttat
    10921 ctggaacaac tgttttcccg tctgcgtaaa aatggtctgg tttccagcgt acgtggacca
    10981 ggcggtggtt atctgttagg caaagatgcc agcagcatcg ccgttggcga agtgattagc
    11041 gccgttgacg aatctgtaga tgccacccgt tgtcagggta aaggcggctg ccagggcggc
    11101 gataaatgcc tgacccacgc gctgtggcgt gatttgagcg accgtctcac cggttttctc
    11161 aacaacatta ctttaggcga actggttaat aaccaggaag tgctggatgt gtctggtcgt
    11221 cagcatactc acgacgcgcc acgcacccgc acacaagacg cgatcgacgt taagttacgc
    11281 gcttaataaa aagaattcag aatcaggccg gagtgctaaa tactccgtaa acacggtcgt
    11341 acatccagcc ggtagcctga ttccttgcat tgagtgatgt acggagttta tagagcaatg
    11401 aaattaccga tttatctcga ctactccgca accacgccgg tggacccgcg tgttgccgag
    11461 aaaatgatgc agttcatgac gatggacgga acctttggta acccggcctc tcgttctcac
    11521 cgtttcggct ggcaggctga agaagcggta gatatcgccc gtaatcagat tgccgatctg
    11581 gtcggcgctg atccgcgtga aatcgtcttt acctctggtg caaccgaatc tgacaacctg
    11641 gcgatcaaag gtgcagccaa cttttatcag aaaaaaggca agcacatcat cactagcaaa
    11701 accgaacaca aagcggtact ggatacctgc cgtcagctgg agcgcgaagg ttttgaagtc
    11761 acctacctgg caccgcagcg taacggcatt atcgacctga aagaacttga agcagcgatg
    11821 cgtgacgaca ccatccttgt ttccatcatg cacgtaaata acgaaatcgg cgtggtgcag
    11881 gatatcgcgg ctatcggcga aatgtgccgt gctcgtggca ttatctatca cgttgatgca
    11941 acccagagcg tgggcaaact gcctatcgac ctgagccagt tgaaagttga cctgatgtct
    12001 ttctccggtc acaaaatcta tggcccgaaa ggtatcggtg cgctgtatgt acgtcgtaaa
    12061 ccgcgcgtac gcatcgaagc gcaaatgcac ggcggcggtc acgagcgcgg tatgcgttcc
    12121 ggcactctgc ctgttcacca gatcgtcgga atgggcgagg cctatcgcat cgcaaaagaa
    12181 gagatggcga ccgagatgga acgtctgcgc ggcctgcgta accgtctgtg gaacggcatc
    12241 aaagatatcg aagaagttta cctgaacggt gacctggaac acggtgcgcc gaacattctc
    12301 aacgtcagct tcaactacgt tgaaggtgag tcgctgatta tggcgctgaa agacctcgca
    12361 gtttcttcag gttccgcctg tacgtcagca agcctcgaac cgtcctacgt gctgcgcgcg
    12421 ctggggctga acgacgagct ggcacatagc tctatccgtt tctctttagg tcgttttact
    12481 actgaagaag agatcgacta caccatcgag ttagttcgta aatccatcgg tcgtctgcgt
    12541 gacctttctc cgctgtggga aatgtacaag cagggcgtgg atctgaacag catcgaatgg
    12601 gctcatcatt aatcggtatc ggaatcagga gaatttataa tggcttacag cgaaaaagtt
    12661 atcgaccatt acgagaatcc gcgtaacgtg ggttcctttg acaacaacga cgagaacgtc
    12721 ggcagcggca tggtgggtgc accggcctgt ggcgacgtga tgaagttgca gattaaagtc
    12781 aacgatgaag gtatcattga agacgcgcgt tttaaaactt acggctgcgg ttctgctatc
    12841 gcttccagct ccctggtcac cgaatgggtg aaagggaagt ctctcgtcga agcgcaggcg
    12901 atcaaaaaca ccgatattgc tgaagaactt gaactgccgc cggtgaaaat tcactgttct
    12961 attctggcag aagacgcgat caaagccgcc attgcggact ataaaagcaa acgtgaagca
    13021 aaataagagt tgaggtttgg ttatgtcgat tacactgagc gacagtgcag cagcgcgagt
    13081 aaataccttt ctggctaacc gcggcaaagg gtttggcctg cgtctgggcg tgagaacctc
    13141 cggatgttca ggtatggctt atgtactgga atttgttgac gaaccgacgc cggaagacat
    13201 cgtgtttgaa gacaaaggcg tgaaagtcgt ggtcgatggc aaaagcctgc aatttctgga
    13261 cggtacgcag ctggacttcg taaaagaagg cctgaatgaa gggtttaaat tcaccaaccc
    13321 gaacgttaaa gatgagtgtg gttgcggcga aagcttccac gtttgatgcg cataccgata
    13381 accccaccgt ggtcgcctgc gcgtggggtt tgttttacct gattcgccgt tatcgcggcg
    13441 gatcgcagcc ctgagaatgt tatggattac ttcaccctct ttggcttgcc tgcccgttat
    13501 caactcgata cccaggcact gagcctgcgt tttcaggatc tacaacgtca gtatcatcct
    13561 gataaattcg ccagcggaag ccaggcggaa caactcgccg ccgtacagca atctgcaacc
    13621 attaaccagg cctggcaaac gctgcgtcat ccgttaatgc gcgcggaata tttgctttct
    13681 ttgcacggtt ttgatctcgc cagcgagcag catactgtgc gcgacaccgc gttcctgatg
    13741 gaacagctgg agctgcgcga agaactggac gagatcgaac aggcgaaaga tgaagcgcgg
    13801 ctggaaagct ttatcaaacg tgtgaaaaag atgtttgata cccgccatca gttgatggtt
    13861 gaacagttag acaacgagac gtgggacgcg gcggcggata ccgtgcgtaa gctgcgtttt
    13921 ctcgataaac tgcgaagcag tgccgaacaa ctcgaagaaa aactgctcga tttttaattt
    13981 ctggaagcta aacatggcct tattacaaat tagtgaacct ggtttgagtg ctgcgccgca
    14041 tcagcgtcgt ctggcggccg gtattgacct gggcacaacc aactcgctgg tagcgacagt
    14101 gcgcagcggt caggccgaaa cgttagccga ccatgaaggc cgtcacctgc tgccatctgt
    14161 tgttcactat caacagcaag ggcattcggt gggttatgac gcgcgtacta acgcagcgct
    14221 cgataccgcc aacaccatta gttctgttaa acgcctgatg ggacgctcgc tggctgatat
    14281 ccagcaacgc tatccgcatt tgccttatca attccaggcc agcgaaaacg gcctgccaat
    14341 gattgaaacg gcggcggggc tgctgaaccc ggtgcgcgtt tctgcggaca tcctcaaagc
    14401 actggcggcg cgggcaactg aagccctggc aggcgagctg gatggtgtcg ttatcaccgt
    14461 tccggcgtac tttgacgatg cccagcgtca gggcaccaaa gacgcggcgc gtctggcggg
    14521 gttgcatgtt ctgcgcttac ttaacgaacc gaccgctgcg gctatcgcct acgggctgga
    14581 ttccggtcag gaaggcgtga tcgccgttta tgacctcggt ggcgggacgt ttgatatttc
    14641 cattctgcgc ttaagtcgcg gcgtgtttga agtgctggca accggcggtg attccgcgct
    14701 cggcggcgat gatttcgacc atctgctggc ggattacatt cgcgagcagg cggacattcc
    14761 tgatcgtagc gataaccgcg ttcagcgtga actgctggat gccgccattg cagccaaaat
    14821 cgcgctgagc gatgcggact ccgtgaccgt taacgttgcg ggctggcagg gcgaaatcag
    14881 ccgtgaacaa ttcaatgaac tgatcgcgcc actggtaaaa cgaaccttac tggcttgtcg
    14941 tcgtgcgctg aaagacgcgg gcgtagaagc tgatgaagtg ctggaagtgg tgatggttgg
    15001 cggttctact cgcgtgccgc tggtgcgtga acgggtaggc gaatttttcg gtcgtccacc
    15061 gctgacttcc atcgacccgg ataaagtcgt cgctattggc gcggcgattc aggcggatat
    15121 tctggtgggt aacaagccgg acagcgaaat gctgctgctt gatgtgatcc cactgtcgct
    15181 gggcctcgaa acgatgggcg gcctggtgga gaaagtgatt ccgcgtaata ccactattcc
    15241 ggtggcccgc gctcaggatt tcaccacctt taaagatggt cagacggcga tgtctatcca
    15301 tgtaatgcag ggtgagcgcg aactggtgca ggactgccgc tcactggcgc gttttgcgct
    15361 gcgtggtatt ccggcgctac cggctggcgg tgcgcatatt cgcgtgacgt tccaggtcga
    15421 tgccgacggt cttttgagcg tgacggcgat ggagaaatcc accggcgttg aggcgtctat
    15481 tcaggtcaaa ccttcttacg gtctgaccga tagcgaaatc gcttcgatga tcaaagattc
    15541 aatgagctat gccgagcagg acgtaaaagc ccgtatgctg gcagaacaaa aagtagaagc
    15601 ggcgcgtgtg ctggaaagtc tgcacggcgc gctggctgct gatgccgcgc tgttaagcgc
    15661 cgcagagcgt caggtcattg acaatgctgc cgctcacctg agtgaagtgg ctcagggcga
    15721 tgatgttgac gccatcgaac aagcgattaa aaacgtagac aaacaaaccc aggatttcgc
    15781 cgctcgccgc atggaccagt cggttcgtcg tgcgctgaaa ggccattccg tggacgaggt
    15841 ttaatatgcc aaagattgtt attttgcctc atcaggacct ctgccctgat ggcgctgttc
    15901 tggaagctaa tagcggtgaa accattctcg acgcagcgct gcgtaacggt atcgagattg
    15961 aacacgcctg tgaaaaatcc tgtgcttgca ccacctgcca ctgcatcgtt cgtgaaggtt
    16021 ttgactcgct gccagaaagc tcagagcagg aagacgacat gctggacaaa gcctggggac
    16081 tggagcccga aagccgttta agctgccagg cacgcgttac cgacgaagat ttagtagtcg
    16141 aaatcccgcg ttacactatc aaccatgcgc gtgagcatta acagaggtta gtatgggact
    16201 taagtggacc gatagccgcg aaattggcga agcactgtac gatgcgtatc ctgatcttga
    16261 tccgaaaacg gttcgattca ccgatatgca tcagtggatt tgcgatctgg aagatttcga
    16321 cgacgacccg caggcatcca acgagaaaat cctcgaagcg attttgttag tctggctgga
    16381 cgaggccgaa taatttccgc gtcatgcttc acgccgcaga tgcgttggct gcgctcgttc
    16441 accccagtca catagttatc tatgctcctg gggcttcact cacttgccgc cttcctgcaa
    16501 cgcgaatcat ttagcggaaa aatcctgggg ctgccaactg gcggcccttt tacaaagaag
    16561 gataactaaa atgacagaag cgatgaagat taccctctct acccaacctg ccgacgcgcg
    16621 ctggggagaa aaagcaactt acagcattaa taatgacggc attaccctgc atttgaacgg
    16681 ggcagacgat ctggggctga tccagcgtgc ggcgcgcaag attgacggtc tgggcatcaa
    16741 gcatgttcag ttaagcggtg aaggctggga tgcggatcgc tgctgggcat tctggcaagg
    16801 ttacaaagcc ccgaaaggca cgcgtaaagt ggagtggccg gatctggacg atgcccagcg
    16861 ccaggaactg gataaccgcc tgatgatcat cgactgggtg cgtgacacca tcaacgcacc
    16921 ggcagaagaa ttgggaccat cgcaactggc acagcgtgct gttgatctga tcagcaacgt
    16981 cgcgggcgat cgtgtgactt atcggatcac caaaggcgaa gatctgcgtg agcaaggtta
    17041 tatggggctg cacaccgtcg gacgcgcttc agaacgttct ccggtattgc tggcgctgga
    17101 ttacaaccca actggcgata aagaagcgcc agtgtacgcg tgcctggtag gtaaaggtat
    17161 cacttttgac tccggcggct acagcatcaa acagactgcg tttatggact cgatgaagtc
    17221 ggacatgggc ggcgcggcaa cggttaccgg ggcgctggca tttgccatta cgcgcggact
    17281 gaacaagcgc gtgaagctgt tcctctgctg tgcggataac ctgattagcg gcaatgcgtt
    17341 caagctgggc gatatcatca cctatcgcaa cggtaaaaaa gttgaagtga tgaacactga
    17401 tgccgaaggg cgtctggtgc ttgccgatgg tctgattgat gccagtgcgc agaaaccgga
    17461 aatgatcatt gatgcggcga ccctcaccgg ggcggcgaaa actgcgctgg gtaatgatta
    17521 tcacgcgctg ttcagttttg acgatgcgct tgccggtcgt ttgctggcga gtgcctcaca
    17581 agagaacgaa ccattctggc gtctgccgct ggcggaattc caccgcagcc agctgccgtc
    17641 taactttgcc gaactgaaca ataccggaag cgcggcgtat ccggcaggcg cgagcacggc
    17701 ggcgggcttc ctgtcgcact ttgttgagaa ctatcagcaa ggctggctgc atatcgactg
    17761 ctcggcgact taccgtaaag cgccggttga acagtggtct gcgggcgcta cgggacttgg
    17821 tgtgcgcacg atagctaatc tgttaacggc gtaaactaat atgccggatg cggcgtaaac
    17881 gccttatccg gcctacaaat tctgcaaatt caacaaattg cgaatccctt gtaggcctga
    17941 taagcgtagc gcatcaggca tttgcgcccc gaacgttgaa tgaattgtgg ttcttatgtc
    18001 cgaaacaaaa aacgaacttg aagacctgct ggaaaaagca gcaaccgaac cggcacaccg
    18061 cccggccttt ttccgtactc tactggaatc caccgtctgg gtgcctggta cggcggcgca
    18121 gggcgaggct gtggttgaag atagtgcgct tgatttacag cactgggaaa aagaagacgg
    18181 caccagcgtc attccttttt tcacctcgtt agaagcgctt caacaggcgg ttgaagacga
    18241 acaggcattt gtcgtaatgc ccgttcgcac attgtttgag atgacccttg gcgaaacgct
    18301 cttccttaat gccaaactgc cgaccggtaa agaatttatg ccgcgtgaaa tcagtttgtt
    18361 gattggtgaa gagggaaatc cgctgagcag ccaggaaatc ctggaaggcg gtgaatcgct
    18421 gatattatcg gaagttgcag agccgccagc acaaatgatt gattcactca ccaccttatt
    18481 taaaaccatt aagccggtga agcgtgcttt tatttgttca attaaagaga acgaagaggc
    18541 acagcctaat ttatttattg gcattgaagc cgatggtgat atcgaagaaa ttattcaggc
    18601 gacgggaagt gtagcgaccg atacattacc tggcgatgaa ccaatcgata tttgtcaggt
    18661 gaaaaaaggg gaaaaaggaa ttagccactt tattaccgaa catattgtgc cattctatga
    18721 acgtcgctgg ggcggttttt tgcgtgactt taaacaaaac cggataatct aaaatatgcc
    18781 gccctaaagg gcggcattcg ggcttacttg ctgctctgaa ggttgttaac caccagtaaa
    18841 ttgtcgccgg agagactccg gttattattt acttgggaaa acagcgcacc agaaacaata
    18901 agtacgatac agaagcaccg tatcttcatt gtgcggtcca tgcgaagacc gcatcacgac
    18961 agcaccactg gcgatattgc cgttagagca agcgctgaga cgcgcttgct ctaacggtcg
    19021 cggaacatcc tttccttaag ccagcgcccg cttgcgagac ggcggaaagg gccgcagtgg
    19081 ataatatact aacagaacaa taataactct cttttgcttg acaaaaagag agttactggc
    19141 gagtattgtc tccgctgaga cgcgcttgca ttactacggt aatacaacct gctggaaagg
    19201 ccgcgaacca gaccagcaat aaaaaaccgc caaattcggc ggttttttat tgcctgtaaa
    19261 atacttattt cactggctca accggtaaat ctgcccgcgc gccccattca ctccatgcgc
    19321 cgtcgtacag tttcacgttt ggcacatcca gcgtcgcgag tgctaacaaa accacggctg
    19381 ccgttacacc agagccgcag ctgacgataa ttggtttgtc gtagctgacg ccgcgaccaa
    19441 aaaatatcgc atccagttca tcggtcgttt ttagttcgcc ttcgcgcacc agttccgtcc
    19501 acggcacatt cagtgctccg ggaatatgtc cgcgacgtaa acctgggcga ggttcatcaa
    19561 cttctgcgtt aaaacgtgca gccgggcggg catcaataat ttgcgccgta ttttcatggc
    19621 ttgccaataa tacatcggtt actttcacca cggcttcagg attaaacgcg gcgttaaact
    19681 ctccttccgg cagctctact gcaccttctt ctaacagcag atcatcgcgc tgccagcctg
    19741 caagtccacc ccccagaatc gacactttct ctacaccaaa ggtgcgcagc atccaccatg
    19801 ctcgtggggc tgagaaaaga ttaccttcgt catagacaat caggtgctta tcctggttaa
    19861 cgcctaattc acgcatcgcc acggcgaacg ttttcgggcg cggcagcatg tgcggaagcg
    19921 gggaagtgtg atcagaaagc gcttcgatat caaaaaacac tgcgccggga atatgtccat
    19981 tcagatactc ctgagcaacg ttacgatctt cctgtccagg cgacgccatg cgggcatcga
    20041 taatctgaat ttccgggtca tcaatatgtt cggcgagcca gtcggctcct acaaaccatg
    20101 tcgtggacat aggcatctcc atttttgccg gggttaggct aaattgtcgg atgtttagtg
    20161 aaaagcgaca agcgaaagag gctgatttgg ctgtctgagc c
//
LOCUS       DAJTNN010000065        20090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTNN010000065 DAJTNN010000000
VERSION     DAJTNN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.065"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            257..799
                     /locus_tag="OO150_002045"
     CDS             257..799
                     /locus_tag="OO150_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516198.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARSTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            899..2101
                     /locus_tag="OO150_002046"
                     /pseudo
     CDS             899..2101
                     /locus_tag="OO150_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
     gene            2321..3103
                     /locus_tag="OO150_002047"
     CDS             2321..3103
                     /locus_tag="OO150_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR5516199.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLSWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKSIACARDISRLLGWKSPFDLQAS"
     gene            3118..4323
                     /gene="rhmD"
                     /locus_tag="OO150_002048"
     CDS             3118..4323
                     /gene="rhmD"
                     /locus_tag="OO150_002048"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCR5516200.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4380..5669
                     /locus_tag="OO150_002049"
     CDS             4380..5669
                     /locus_tag="OO150_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5516201.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5687..6490
                     /gene="yfaU"
                     /locus_tag="OO150_002050"
     CDS             5687..6490
                     /gene="yfaU"
                     /locus_tag="OO150_002050"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR5516202.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6531..7433)
                     /locus_tag="OO150_002051"
     CDS             complement(6531..7433)
                     /locus_tag="OO150_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5516203.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(7626..8816)
                     /gene="glpC"
                     /locus_tag="OO150_002052"
     CDS             complement(7626..8816)
                     /gene="glpC"
                     /locus_tag="OO150_002052"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5516204.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8813..10072)
                     /gene="glpB"
                     /locus_tag="OO150_002053"
     CDS             complement(8813..10072)
                     /gene="glpB"
                     /locus_tag="OO150_002053"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5516205.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLH
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLLCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10062..11690)
                     /gene="glpA"
                     /locus_tag="OO150_002054"
     CDS             complement(10062..11690)
                     /gene="glpA"
                     /locus_tag="OO150_002054"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5516206.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11963..13321
                     /gene="glpT"
                     /locus_tag="OO150_002055"
     CDS             11963..13321
                     /gene="glpT"
                     /locus_tag="OO150_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5516207.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13326..14402
                     /gene="glpQ"
                     /locus_tag="OO150_002056"
     CDS             13326..14402
                     /gene="glpQ"
                     /locus_tag="OO150_002056"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5516208.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14444..14686)
                     /locus_tag="OO150_002057"
                     /pseudo
     CDS             complement(14444..14686)
                     /locus_tag="OO150_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14865..15515
                     /gene="inaA"
                     /locus_tag="OO150_002058"
                     /pseudo
     CDS             14865..15515
                     /gene="inaA"
                     /locus_tag="OO150_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(15569..15823)
                     /gene="yfaE"
                     /locus_tag="OO150_002059"
     CDS             complement(15569..15823)
                     /gene="yfaE"
                     /locus_tag="OO150_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5516209.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15823..16953)
                     /gene="nrdB"
                     /locus_tag="OO150_002060"
     CDS             complement(15823..16953)
                     /gene="nrdB"
                     /locus_tag="OO150_002060"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5516210.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17100..19385)
                     /gene="nrdA"
                     /locus_tag="OO150_002061"
     CDS             complement(17100..19385)
                     /gene="nrdA"
                     /locus_tag="OO150_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5516211.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGECP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4709 a         4861 c         5425 g         5095 t
ORIGIN      
        1 aagaaaccat gagaatacgg taactcagaa atatgtgaaa gtgacagtaa tctcttttgc
       61 gatgataact gcacttatta ttatttttgt gtgtatcggt aaagtgtgtt tattttttcg
      121 gcgtttaatt atttaagaag tatttagtta caataagtga ctagatccct tttttcgctc
      181 attgaatcct ctgtgccagg cagtacaatc ccgcagcttt gggaattttg attaattgct
      241 tgtgggaaag gggataatga aaaaaattgc gctagcaggt ctggccggaa tgctgttggt
      301 ttctgcatcg gtcaatgcaa tgagcatcag cggtcaggcg ggtaaagaat acaccaatat
      361 tggtgtcggt tttgggactg aatcgacggg cctggcgtta agcggtaact ggacacataa
      421 cgacgacgac ggtgacgtcg ctggcgtggg gctggggttg aatctgcctc tagggccgtt
      481 aatggcgacc gttggcggaa aaggcgttta caccaacccg aattacggcg atgaaggtta
      541 tgccgcagcg gtaggaggtg gtttgcagtg gaaaattggc aacagcttcc gtttgtttgg
      601 cgagtattac tactctccgg attcactctc cagcggtatt caaagttatg aggaagcgaa
      661 tgctggcgcg cgttccacca ttatgcgtcc agtcagtatt gaggcgggtt atcgctacct
      721 gaatttgtcg ggtaaagatg gtaaccgcga caacgccgtg gctgacggcc cgtatgttgg
      781 ggttaacgcc agtttctgat cccttccggc gcggcattct gtcgcgccat tcagtttctt
      841 actacgcctt atctcccttt gcgttatagt gattcgataa caaaagcagg agataaccat
      901 gttaaaagtg gaaatgttat ccaccgggga tgaagtgtta cacgggcaaa tcgttgacac
      961 taacgctgcc tggctggccg attttttctt tcatcagggg ctgccattat ctcgcctcaa
     1021 tacggtgggg gataaccttg atgacttagt caccattctt cgcgaacgta gtcagcacgc
     1081 cgatgtgctg atcgttaacg gcgggctggg accgaccagc gatgatttaa gcgcactcgc
     1141 cgctgcgaca gcaaaaggtg aaggcctggt gctgcatgaa gcctggctca aagagatgga
     1201 acgctatttc cacgaacgtg gacgagtaat ggcaccgagc aaccgtaaac aagcggagct
     1261 gcctgccagt gctgaattta tcaataaccc ggtaggcacc gcctgtggtt ttgccgtgca
     1321 gcttaatcgt tgcctgatgt tctttactcc cggcgtaccg tcagaattta aggtgatggt
     1381 cgagcacgaa atcctgccgc gcctgcgcga gcgtttttct ttaccacagc cgccggtttg
     1441 tctgcgttta actacctttg gtcgttcgga aagcgatctg gcacaaagcc tggatactct
     1501 acaactgccg ccgggcgtaa caatgggcta tcgctcttca atgccgatta tcgaactgaa
     1561 actcaccgga ccggcaagcg agcaacaggc gatggaaaaa ctgtggctgg acgttaaacg
     1621 tgttgccgga cagagcgtga ttttcgaagg caccgaagga ctgcccgtgc agatcagtcg
     1681 cgaattgcaa aaccgccagt tcagcctgac gttgagcgag taattcaccg gtggtttatt
     1741 ggctttgcaa ctttctcgcg caggtgctcc attgctggcg tgtgaagtgg ttccttcaca
     1801 ggaagaaaac ctggcgcaaa ctgcgcactg gattacagaa cggcgggcca accattttgc
     1861 cgggctggca ctggctgttt cgggtttcga gaacgaacat ctcaactttg cgctagccac
     1921 accagacggc actttcgctc tgcgtgtgcg tttcagcact acgcgctaca gcctggctat
     1981 ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgctg cgccgttggt taaacggcca
     2041 ggatatcgcc agtgagcatg gctggattga ggttattgag tccatgacct tatctgtctg
     2101 attccgccac tctgtgccgg tttactgcac cggcacattc cccctccggc aaaacttgtc
     2161 caacctgttg cactcaataa gaaaaaatcc gtgcactgat tcacaaattt gtgcgtaatt
     2221 tctccagtcc gctggacgag gagcgggatt tgatgcgctt cacaatttta ctccccctga
     2281 gcaacgagac tgattccagt atctaagact ggagtccagt atgttggaat caagcaaagt
     2341 tcccgccctg acacgggcta ttgatattct caatttgatt gcccgcattg gcccctgtag
     2401 tgctgcgacg atcattgaca cattgggaat ccctaaaagc acggcctatt tgctgcttaa
     2461 tgaactcagg cgtcagcgtt ttcttagcct tgatcaccag gaaaattttt gtttgtggac
     2521 caggctggtg gagctttctg gtcatgcgtt aagcaagatg gatctccggg aactggctcg
     2581 accgcgtttg acgcaactga tggataccac cgggctgtta tgtcatctgg ggattattga
     2641 taacggcagc gcttattaca ttttaaaagt ggagtcatca gccactatca gcgtgcgttc
     2701 ccatgaagga aaaagccttt cgctttatcg ctccggaata ggtaaatgcc tgctttcctg
     2761 gcaacctgca gcggtacagc aaagtattat cgaagggctg gtctgggagc aggcgacacc
     2821 cacaacgatt actcatccac agcaacttca cgaagaactg gcgcgtattc gccgtcaggg
     2881 ctggagctat gacaacggcg aagattaccc cgatgtccgt tgtgtcgctg cgccggtgtt
     2941 taacgccaat aacgagctta ccgctgcgat ctcggtggtc ggtacccgct tacaaatcaa
     3001 tgaagaatac cgtgattatc tggcgggcaa gtccattgcc tgcgccaggg atatttcacg
     3061 tctgttggga tggaaaagtc cctttgactt acaagcctca taacaatgga gaacatcatg
     3121 accctaccaa aaattaaaca ggttcgcgcc tggtttactg gcggtgcgac agcagaaaaa
     3181 ggcgctggcg gcggtgatta tcacgaccag ggggcgaatc actggataga cgatcatatt
     3241 gccaccccga tgagtaaata ccgcgattac gagcagtcac gccagtcatt tggcattaac
     3301 gttcttggca cgttggttgt tgaagtcgaa gcagaaaacg gccagaccgg attcgctgtt
     3361 tcgacagccg gtgaaatggg ctgttttatc gtcgaaaaac accttaaccg tttcattgag
     3421 ggtaaatgtg tcagtgatat caaactgatc cacgatcaaa tgctcaatgc caccctgtat
     3481 tactccggct ctggtggctt ggtgatgaat acgatttcct gtgtcgatct ggctctgtgg
     3541 gatctgttcg gcaaagtggt cgggcttccg gtttataaac ttttaggcgg cgctgttcgt
     3601 gatgagattc agttctacgc cacaggtgcg cgtccggatc tggcaaaaga gatgggcttt
     3661 atcggtggca aaatgccgac gcactggggg ccacatgatg gcgatgcggg gatccgcaaa
     3721 gatgccgcta tggtcgcgga tatgcgtgaa aaatgcggtg aagatttctg gttaatgctc
     3781 gactgctgga tgagtcagga cgtgaactat gcgaccaaac tagcccacgc ttgcgcgccc
     3841 tataacctga aatggatcga agagtgcctg ccgccacagc agtatgaagg ttatcgcgaa
     3901 ctgaaacgca acgcgccagt cggaatgatg gtcaccagcg gtgagcacca cggcacactg
     3961 caatcttttc gtacgctttc agaaaccggt atcgacatta tgcagccgga tgttggctgg
     4021 tgcggtggtt taaccacgct ggtggaaatt gccgcaatcg ccaaatccag ggggcaactg
     4081 gtggtgccgc acggttcgtc tgtttactct caccatgcgg tgatcacctt caccaatacg
     4141 ccattcagcg aattcctgat gaccagcccg gattgttcaa cgatgcgtcc gcagtttgac
     4201 ccgattctgc tcaatgagcc ggttccggtg aatggtcgta ttcataaatc agtgcttgat
     4261 aaacccggtt ttggcgtcga actcaatcgt gactgcaatt tgaaacgccc ctacagccac
     4321 taatcactcc gggcgttgct gtgacaacgg taacgcccac atattctaag gagcttttta
     4381 tgagcaccgc tttgcttgac gccgtggtga agaaaaaccg tgtgcgttta attccgttta
     4441 tgttggcgct gtatgtgctg gcgtttctcg accgttcgaa tatcggtttt gccaaacaga
     4501 cctaccagat tgataccggg ttgagtaatg aagcttatgc gctgggagca ggcattttct
     4561 ttgtggtata tgcgtttctg ggtgttccgg cgaatctttt gatgcgcaaa ctgggggcca
     4621 gaacctggat tggtacgaca acactgctgt ggggatttct ttcggcagcc atggcatggg
     4681 ccgatactga agcgaaattt ctgatagttc gcactctgct tggtgctgcg gaggccggat
     4741 ttttccctgg tatgatttat ctcacctcgc aatggtttcc gcagcgtaat cgcgccagca
     4801 ttatggggct gttctatatg ggcgcaccgc tggcgttaac actgggatca ccgctttccg
     4861 gcgcgctgtt ggagatgcat ggatttatgg ggcatcccgg ctggttctgg atgtttgtga
     4921 ttgaaggatt gttggcagtc ggtgctgggg tattcacatt cttttggctt gatgacacac
     4981 cggagcaggc acgttttctg agtaaacaag aaaaaacgtt gcttatcaat caactggcaa
     5041 gtgaagaaca acagaaagtg acttctcggc tgagcgatgc gctgcgtaat ggccgagtct
     5101 ggcaactagc gattatctac ctgaccattc aggtggcggt ttacggatta attttcttcc
     5161 tgccgaccca ggttgcggca ttgctgggaa caaaagtggg ctttacagcg tcggtggtca
     5221 ccgccattcc gtgggttgcg gccttgtttg ggacctggct tattccgcgc tattccgata
     5281 aaaccggcga acggcgtaat gtcgcagcgc tgacattact ggcggcgggc attggtattg
     5341 gtctgtccgg gctgctttct ccagtaatgg cgatcgtagc gctgtgtgtt gcagctatcg
     5401 ggtttattgc cgtgcagccg gtgttctgga cgatgccgac acaactgctt tccggtacgg
     5461 cgctggctgc ggggattggt tttgtaaacc tgtttggtgc agtgggcggg tttattgccc
     5521 cgatcctgcg cgtgaaagca gaaacgttat ttgccagcga tgcggcagga ttactgacac
     5581 tggcagcggt ggcggtcatc ggttcgctga ttattttcac tctgcgtgta aatcgtactg
     5641 ttgcgcagac cgacgtggca catcattaaa taggttaagg aacacgatga acgcattatt
     5701 aaccaatccc tttaaagaac gtttacgcaa gggcgaagtg caaattggtc tgtggttaag
     5761 ctcgactact tcttatatgg cagaaattgc cgctacttcc ggttatgact ggttgctgat
     5821 tgacggggag cacgcgccaa acaccattca ggatctttat caccagctac aggcggtagc
     5881 gccctatgcc agccaacccg tgatccgtcc ggtggaaggc agtaaatcgc tgattaaaca
     5941 agttctggat attggcgcgc aaactctatt gatcccgatg gtcgatactg ccgatcaggc
     6001 tcgtcaggtg gcgtctgcca cgcgctatcc tccctacggt gagcgtggtg ttggggccag
     6061 tgtggcacgg gctgcgcgct ggggacgcat tgagaattac atggcgcaag tcaacgattc
     6121 gctttgtctg ttagtgcagg tggaaagtaa aacggcactg gataacctgg acgagatcct
     6181 cgacgtcgaa gggattgatg gcgtgtttat tggaccagcg gatctttctg cgtcgttggg
     6241 ctacccggat aacgccgggc acccggaagt gcagcgaatt attgaaacca gcattcggcg
     6301 gatccgtgct gcgggtaaag cggctggttt tctggctgtg gctcctgata tggcgcagca
     6361 atgcctggcg tggggagcga actttgtcgc tgttggcgtt gacacgatgc tctacagcga
     6421 tgccctggat caacgactgg cgatgtttaa atcaggcaaa aatgggccac gcataaaagg
     6481 tagttattga tatcaaaggc ccatggggat cggctgtggg cctgtgttaa ttagtggtta
     6541 ttcgctgcca gatcggcttc gcttagctgg gtggccgcga gcacctggtc acgatcaatg
     6601 ccctgttcca gcatgcgcaa agcaatttta atggcttgtt catgcatacc ttcctgccag
     6661 ccaatttgat gcccttcctg ccgtagccgt tcagcaatag tcattaatct ctccttttgt
     6721 agtggtgaac gttcggcaat ctcctcaata aaacgggtga aacgggaggt atcgccgcat
     6781 tgcagcagat aattaaacag tgtttgtagc tggctgtcat tagtgaagcc tctaaccaaa
     6841 agcgtggtga tcctgtcgac catgccgatt aaatcgcggt cgcgaatatg cttttgaatc
     6901 agttccagca gagctatacg ccgatgttgc atgatctcat cgtcaggtac gatggtgata
     6961 tccaccaacg gaaaagcttc ggtgtacaac tgccgggcaa gttgcggatc gtcaaactca
     7021 tccagccagt taagtgagta cgggtagggc gatgtttcgc catgataaaa cagcaatggc
     7081 accaccagcg gaactctgtc atagccttta tccaggtgac gctgcatggc ggcagtggca
     7141 tagcgcatta gccgaaaagc catattcttt tctgcagagc tttgatgttc aatcacgcag
     7201 tagatatagc cgtcaccgtc gctggtttcc acggaccaca aaacatccga gtagtaagcg
     7261 cgtaaacttt tttcaataaa actagtgggt tccaggcgta aggtttgcag gttgcaaagc
     7321 ttgcgcagtg gttctggtaa atgtatttcg agaaaatccc gtgcggtttc gggtgtgaac
     7381 ataaaggttt taaataccgc atcatgcggg gaggaggttg ttgattcggt catggttgtc
     7441 cgtcacctga tatttatgat gacgcgacat tacggttatc ctaggccttt gacatctgcc
     7501 agtttcagcc tttgcgagac gttttcagag gaattttcac tgcgttgcaa cgcatacatt
     7561 gggcacggaa tcgtgtacgc aaaaatgccc ggtaaccaca tgcttaccgg gcatcagaaa
     7621 ggagtttaag ccagcgcctg ggccagtagc gtaatcggat gttcgcagcg aagacttgtg
     7681 gacatctcaa tctgccattt acaggtttcg cagtcggtga ccaccagatc tgcgccgctt
     7741 tcttctatct ggcggaacag tggtgcgccg atggcttgtg aggtggggta gttctctttt
     7801 ttgaaaccgt aagtacccgc aataccgcag cactgggaat ccagcaccgt taactcaagc
     7861 cccgggattt tacgcaacag ctccagggtg tagagcgtcc agcccatttt ttccatatgg
     7921 cacggagtgt gataaaccac tttcagcggc agcggtttca gcggtaacgt tttgccttcg
     7981 tccagcttgc gccacagcca gcgggttgcc agttcgatat gatcgcgcaa gcctttgttg
     8041 tcgacattca gcacttccgg gtattcgtcg cgcagggcaa atgtacaggt tgaggaggtg
     8101 gcaatcaccg gaatgccttt tactcccaca gcttcgcgga tcgactctac attcgtaatt
     8161 gcctgtttgc gtgctttatc ggtaaagccg ttggcgatta gcggtacgcc gcagcatttt
     8221 tctttgctga gcagttgtac accggtaccc attgcgttga gcactttaat taaatcttta
     8281 cctaactgcg gatggttgta gttaacgaag cagccgtgaa agaaagcgac ctggtcttta
     8341 tattgtgctt gctgagccgc tatgctgcga taccagcgac ggaacgtgcc gaaggagtat
     8401 ttcggtagcg tgcgacgatg atcgattttt aacgccgcat caagcagctg ccgcaccggt
     8461 ttcagcgagg tagcggtgtt gacgattggt gcgaacggcg tggaaacgct acccatcagg
     8521 tcggtatgac tcaacacaaa attacgcagc gacgggcgcg tggtgtcata ttttgcccgc
     8581 gcgcgctgga taatatcgcc aatcttcaca tcggacgggc aggcgacttc acaacgtttg
     8641 cagttgatgc aatatttcag cgcctcgtca tacagtgcgc catctttcaa acgcagacgc
     8701 tcgccatccg gcccggcttg ttttggccct ggataaccgg gattcacccg gctcaccggg
     8761 caggcggtgg tgcagacggt gcacttaatg cagttttcga agctggtgtc gttcattgtt
     8821 ggcctcctgc gcgttgggca atctgttgag cggcatgtaa agcactgacg gcacaaacac
     8881 cgccgccgca tccctgggcg atgggatcaa atccgcccag caccgaaccg atggcaaaca
     8941 ggttctcaat ggtttgccct gcctgcgacg ggcgtagcgt ctcatcagtg gttacaccga
     9001 actgctgcca cggttgcggc gcaaaaaaat cgcccttata ccattcaccc cgcgtggcgg
     9061 tttgtagcac atcaaggccg agaatcggct cgcgaatgcc gttacgttcc gctaccagtc
     9121 cgccactaaa gaaactgccg ctggcgagaa ccgcgaaatg tggacgtagc ggaatatcgg
     9181 cgtgattgcg ggtccagatt tcattcacta cgccattttt acaggtcact tttttcactt
     9241 catcgcccgg catccacacg ccaccctggc gcacaaactg gcgctgtaac tggttttgca
     9301 gacgaatgcc cagcacggaa ggcggcagcg ttggcaaaag catcagtgaa caaagtagtt
     9361 tttcattcaa ccaacgccac agtttgtcat cggccaggcc gaagcaggca ggcatcagga
     9421 tcatttcgca ggtattggcg acaggaataa gcgcatcaag taacagcggc cagttttctt
     9481 cattatcaag gaaacgggcg atattcacag cgcgaaattc ggtggcgtta ttgcgcagga
     9541 catccagttc cggcagctct atttctgcgg tttcaaccga aagatccagt tcgtgcaacg
     9601 aagccgccgc aagatgcgcc tgaaaatcca tcaggccgct tatcccgacc acacatattt
     9661 tcttcgcggg caacggccag acggggactt ctggcgaact tagccaggta gagcgcagag
     9721 tgccgagcgg cgtaatccgc tggtgagcca gttctacgct gccctgcaat tgcgcaccgc
     9781 tttcagcgat taatgcctgc gcctggcaag cgagatcgag cacgcgttgt ggcccgagaa
     9841 gagagtaagg atgtgcaggt gcctgctgac gcaaagattc cagtccactg tgaatgtctg
     9901 ccacgggttg accatctggc agatggctca gcaaatccag cgaaccggat gagaaatgca
     9961 acgcgctttg accacgcgtg acaatggcac agcgcaggcc gtgtttttgc agttgcaggc
    10021 cacagagtaa tccggcgagg ccgccgccca taatgacagt atcaaagcgc atctttctgc
    10081 tccttctcca gaccacacag tccctgataa acccagcggg taaattcgct ttcgcgcagt
    10141 gcgtctcccc aggcgatggg ttgcacgcct ttccagcgtt cgttaaggaa ggtggaaagt
    10201 tgctcgatag attgcgcgga cgtcgtgacg ttaaaccgtt gcagcagtcc ggcagcgcgg
    10261 caggcgcaga gttcgccctg gcaggtgccc atccccacac gggtacgacg gcgtaaatcc
    10321 agcaggctat taacgtttaa attttctacc gcgtactgca cttcacccgc agttaccgct
    10381 tcgcactcac ataccaggct acggtgcaga cggccttcgc tcagccaggc aggcgtgcga
    10441 tcgccatgac gataaaccgc agaaccgcgc agcggggcag gcagggagat gactttacgc
    10501 aaggtaactt cagcgggttc ttgtgaacca ggcagtgcca gatcggcagt cgtacagggg
    10561 cgcgtgttgc ccagtttgcg gcataccgcg tcggtagccc attcagccat cagccgatag
    10621 gtcatcagtt tgccaccggt gatggtgata aatccgtcca gaccatcgcg ttcagcatgg
    10681 tcgagcagca cgatgccacg gctgacgtta cgtccgctcg gatcgtcatc gctggcaacc
    10741 agcgggcgca cgccagaata ggcccgcaaa atgcgcgttt tcgccatcac gggggccagt
    10801 ttttcccctt cacgcagcag aatatcaacc tcttctgccg tcactcgatt atcgtcaatc
    10861 tcgttgtaat caatacgtaa agaggtggta ccaatcagcg aaatggtatc gccaggcacc
    10921 agaatatcgg cgtcggaagg tttacggcag cggttgatca catgctggtt aatgcggtga
    10981 tccatgatca gcagcgatcc tttcgccggg aacatgcgaa tgcgcagatc ggcatattcg
    11041 gcaatgtgtt gcccccagat cccagcggca ttaaccacga caggtgcatg aagggcctga
    11101 gtttcgccgg tgagatggtt acgtacacga acaccgcaca ccgtcgcgcc ttcacgaatc
    11161 agccccgtga cttcatgagc ggtaaggata acggcaccgt gttctttggc atccagcatg
    11221 tttgctgcgg tcagacgaaa tggatcaacg gtgccatccg gaactttcac cgcgccaatc
    11281 agtgccgggt taacggcagg ttcgataatg cgcgcttgct gcgggtctat agcttctgcg
    11341 ctgatccctg cttcttcgca ggcgcgaata aaagtggcct ggaatgagag gtcatcttcc
    11401 ggcagggtga taaacaggcc gttggttggt tcaacgcagt gacgtgcaat gcgtttcagg
    11461 atctggtttt cactaatgca ttcgcgggcc gattccgcat cggttaccgc atagcgcgca
    11521 ccgctgtgca gcaggccgtg gttacgcccg gtggcaccgg ttgcgatgtc gtggcgctca
    11581 accaaaatca cgcgcagccc gcgcagggca cagtcgcggg caatcccggc tcccgttgcg
    11641 ccgccgccaa tgataatcac gtcacttgat tgcgagtcgc gagttttcat tgttatccct
    11701 ctgaagttcg ttttttacca tttagccata gtaaaaacat gaattgtttg atttcgcgca
    11761 tgttcgctca taattcgaaa gtgaaacgtg atttcatgcg tcattttgaa cattttgtaa
    11821 atcttattta ataatgtgtg cggcaattca catttaattt atgaatgttt tcttaacatc
    11881 gcggcaactc aagaaacggc aggttctctc actgaatcag gctgttaatc ataaataaga
    11941 ccacgggcca cggaggctat caatgttgag tatttttaaa ccagcgccac acaaagcgcg
    12001 cttacctgct gcggagatcg atccgactta tcgtcgattg cgctggcaaa ttttcttggg
    12061 gatattcttt ggctatgcgg cttactattt ggttcgtaag aactttgcgc ttgctatgcc
    12121 ttatctggtc gagcagggat tctctcgcgg tgatttaggt ttcgcgcttt cggggatctc
    12181 gatcgcttat ggattttcga aattcataat gggttcggta tcagatcgct cgaatccgcg
    12241 cgttttcctc cccgcaggtt tgattctggc ggcggcagtg atgttgttta tgggctttgt
    12301 gccatgggcg acgtcgagca ttgcggtgat gtttgtactt ttgttcctct gcggttggtt
    12361 ccaggggatg gggtggccgc cgtgtggtcg tactatggtg cactggtggt cgcagaaaga
    12421 acgtggcggc attgtgtcag tgtggaactg tgcgcacaac gttggtggtg gtattccgcc
    12481 gctgctgttc ctgctgggga tggcctggtt caatgactgg catgcggcgc tctatatgcc
    12541 tgctttctgc gccattctgg tggcattatt cgcctttgcg atgatgcgcg ataccccgca
    12601 atcctgtggc ttgccgccga tcgaagagta caaaaatgat tatccggacg actataacga
    12661 aaaagcggaa caggagctga cggcgaagca aatcttcatg cagtacgtac tgccgaacaa
    12721 actgctgtgg tatatcgcca tcgccaacgt gttcgtttat ctgctgcgtt acggcatcct
    12781 cgactggtca ccgacttatc tgaaagaggt taagcatttc gcgctggata aatcctcctg
    12841 ggcctacttc ctttatgaat atgcaggtat tccgggcact ctgctgtgcg gctggatgtc
    12901 ggataaagtc ttccgtggca accgtggggc aaccggcgtt ttctttatga cactggtgac
    12961 catcgcgact atcgtttact ggatgaaccc ggcaggtaac ccaaccgtcg atatgatttg
    13021 tatgattgtt atcggcttcc tgatctacgg tcctgtgatg ctgatcggtc tgcatgcgct
    13081 ggaactggca ccgaaaaaag cggcaggtac ggcagcgggc tttaccgggc tgtttggtta
    13141 cctgggcggt tcggtggcgg cgagcgcgat tgttggctac accgtggact tcttcggctg
    13201 ggatggcggc tttatggtaa tgattggcgg cagcattctg gcggttatct tgttgattgt
    13261 tgtgatgatt ggcgaaaaac gtcgccatga acaattacta caaaaacgca acggaggcta
    13321 atggcatgaa attgacgctg aaaaacctta gcatggcgat catgatgagc actatagtca
    13381 tgggaagcag tgcaatggcg gcggacagca acgaaaaaat agtcatcgcc catcgcggtg
    13441 ccagtggata tttgccggag catacgctgc cagcaaaagc gatggcgtat gcgcagggag
    13501 cggattatct ggaacaggat ttggtgatga ccaaagacga ccatctggtt gttctgcatg
    13561 accattatct cgatcgtgtt actgatgttg ccgatcgttt cccggatcgg gcgcgcaaag
    13621 acggtcgtta ctacgcgata gatttcacgc tggatgaaat taagtcgttg aaatttaccg
    13681 aaggtttcga tattgaaaac ggtaaaaaag tgcagactta tccggggcgt ttcccaatgg
    13741 gtaagtccga cttccgggtg cacacctttg aagaagagat tgaatttgtt caggggttaa
    13801 atcactctac cgggaaaaat atcggtattt atccagaaat caaagcgccg tggttccatc
    13861 atcaggaagg gaaggatatt gcggcaaaaa cgctggaagt gctgaagaaa tatggttaca
    13921 ccggtaaaga cgataaagtt tatttgcaat gttttgatgc tgatgagctg aagcgtatta
    13981 agaatgagct ggaacccaaa atgggcatgg agctcaatct ggtacagctg attgcctata
    14041 ccgactggaa tgaaacgcag cagaaacagc cggatggaag ctgggttaat tacaactacg
    14101 actggatgtt taagccgggt gccatgaaac aggtggcgga atatgcagat ggtattggtc
    14161 cggattacca tatgttgatt gaggagacat cgcagccggg taatatcaaa ctcactggca
    14221 tggtgcaaga tgctcagcag aataaactgg tagtgcatcc ttataccgtg cggtcagata
    14281 aactgcctga atacactcct gatgtgaatc agttatatga tgctctgtat aacaaagcgg
    14341 gtgtaaatgg gctgtttact gatttccctg ataaggcagt aaaatttctt aataaagagt
    14401 aatgttactg caggatggcg ttcatgcgcc atcctgtctc atatcaaatt tttccaatcg
    14461 ccactaattg ttccatgcac atttgtagta aaaacagtgg actgccttct accggctttt
    14521 ctttagccag caacgaaatc tgtcgataga aagttggttc gagaggaacg gaacacaatt
    14581 cacctgcaat gcttttcagc gttaactctg gttgtaaggc gatgcctaat ccctgtcgaa
    14641 taaaattcat cgctgtatcc gcctgattaa attcacattt tatctgctgg gcgcactatt
    14701 agtggtagtg gctggtggaa tctatgcgct gcaaatgaag ggtaaaagcg gtgtcaagct
    14761 agtagtggca aaagaaattc attaatacga cacgtttcat taagattttc ctcaggtaac
    14821 cacctatagt cattgtcgtt aataaaaacg acaggcgata gggtatggca gtttcagcaa
    14881 agtacgacga atttaatcac tggtgggcaa cagagggcga ctgggttgaa gagcctaact
    14941 atcgccgtaa tggtatgagc ggggtgcaat gcgtcgagcg caacggcaaa aagctgtatg
    15001 taaagcgcat gacgcatcat ctgtttcatt ctgtacgtta tccgttcggc cgaccaacga
    15061 ttgtccgtga ggttgcggta attaaataac tggaacgggc cggtgtcatc gtgccgaaga
    15121 tcgtttttgg tgaagcggtg aaaattgagg gtgaatggcg agcgttgctg gtgactgaag
    15181 atatggcggg gttcatcagc attgctgact ggtatgccca gcatgcagta tcgccttatt
    15241 ctgacgaagt acggcaagcc atgttgaaag cagtggcgct ggcgtttaag aaaatgcata
    15301 gcattaatcg tcagcatggc tgttgttatg ttcgccatat ctatgtgaaa acagaaggta
    15361 aggcagaagc tggttttctt gacctggaga aaagtcgtcg ccgcttgcgt cgcgataaag
    15421 cgattaatca tgacttccgc cagctggaaa aatatctgga gccaatcccc aaagcggact
    15481 gggagcaggt gaaagcgtat tactacgcca tgtaatcgtt gtaggccaga taagacacat
    15541 aagcgttgta tctggcacta caaccaattc acatctcgat ttcaatatcg ccttttgccc
    15601 ggcaacaaca gggcaaaatt tcccccggct gaataaaggc taacggttcg gcaatccagt
    15661 caacttgacc tgcaaccagg cgtgtgcgac aggagccgca gtaaccttcg cgacactggt
    15721 actcaaccgc cacattgtgg gattccagcg ctgccagaag ggaagggtgt tcatcctggc
    15781 acagcagttg tgtgccagtg atgcgcaggg taacgcgggc catcagagct ggaagttact
    15841 caaatcgtcg gtgtccactt ccgagtcaat ctgcccgacc agataagaac tgacttccac
    15901 ttcctgcgga gcaacctgca cgttatcaga caccagccaa gtgttgatcc acgggatcgg
    15961 gttggagcgc gtctggaacg gcagatccaa accgactgcc tgcatacgga tattggtgat
    16021 gtattcaacg tactggcaga gaatgtcttt attcagacca atcatcgaac cgtcgcggaa
    16081 cagataatcc gcccagtctt tctcctgttg agctgcctga acaaacaggt catagcactc
    16141 ctgcttacac tcttcggcaa tttccgccat ctcaggatcg tccgcgccgc tgcgcagcag
    16201 attcagcata tgctgggtgc cggtcaggtg cagggcttcg tcgcgggcaa tcaggcgaat
    16261 aattttggcg ttgccttcca tcaattcgcg ttctgcaaag gcaaaggaac aagcaaagct
    16321 gacgtagaag cggatcgctt ccagcgcgtt aacgctcatc aggcaaagat acagtttttt
    16381 cttcagctcg cgcaggctaa cggttacggt tttaccgtta acggtgtggg tgccttcgcc
    16441 cagaaggtgc cagtagctgg tcatttcgat cagttcatcg taatagctgg agatcccttc
    16501 cgcacgtttc tggatctgct cgttggtgac gatatcgtca aacacaacag acggatcgtt
    16561 aacgatatta cgaatgatat gagtatagga acgggaatga atcgtttctg agaacgccca
    16621 ggtttcgacc caggtttcca gttccggaat agaaataagc ggcaatagcg ccacgttcgg
    16681 actacgaccc tgaatggaat ccagcagcgt ctgatatttc aggttgctga taaaaatgtg
    16741 tttttcgtgc tccggcagcg cctggtaatc gatacggtcg cgggagacgt caacttcttc
    16801 cggacgccag aagaaagaga gctgcttttc gatcagcttt tcgaagatgt catatttttg
    16861 ctgatcgtag cgggccacgt tgaccggctg accaaagaac atcggttctt tgagctgatc
    16921 atttttcgtc tgtgaaaagg tggtatatgc catgagtgtg tcctgttggg agtttaatgc
    16981 cggataaggc attttacgcc gcatcctgtg cctgatgcga cgctggcgcg tcttatcagg
    17041 cctacaaatc cgagccgtag gccggataag gcgttcacgc tgcatccggc atctcaatat
    17101 cagatcttac atgcgccgct ttcgcagcca tcgtcctgga ttgacggtac cagatcgtcc
    17161 tgcgcgtctt cagcgccgtc acgggtgttc tgataataca gcgttttgac cccgaatttg
    17221 taggcggtga gcaggtcttt cagcaactgc tgcatcggca cttttcctga cgggaaacgt
    17281 gacggatcgt agttggtgtt agcagagatc gactgatcga taaatttctg catgatacct
    17341 accagttgca ggtagccatc gttacccggc atttcccaca gcagctcata ggcgtcgtgc
    17401 aggtgctcgt agtccggcac cacctggcgc aaaataccgt ctttcgacgc tttgatgctg
    17461 acgtaaccgc gcggcggttc aataccgtta gtggcgttag agatctgcga agaggtctcg
    17521 gacggcatca gggcagaaag cgtggagtta cgcaggccgt gcgttttgat tgattcacgc
    17581 aatgcttccc agtcgtaatg cagcggctca ttagcgatgg catccagatc tttcttatag
    17641 gtatcgatcg gcaggatccc tttcgcgtaa gtggtttcgt taaaccacgg gcactcgcct
    17701 tgctctttag ccagctcatt agacgctttc agcaggtaat actgaatcgc ttcgaaggtt
    17761 ttatgcgtca ggttgttggc gctgccgtcg gagtagcgtt taccgtgctt cgccaggtag
    17821 taagcgaagt tgatcacacc aatacccagc gtacgacgac ccatcgctcc acgtttggcg
    17881 gccgggatcg ggtaatcctg ataatccagc agcgcgtcaa gtgcacgaac cgccagaatt
    17941 gccagctctt ccagttcatc caggttatta attgcgccca ggttgaaagc agacagcgta
    18001 cacagcgcga tttcaccgtt ctcgtcgttg acgtcgttca gcggtttggt cggcagggct
    18061 atctccaggc acaggttaga ctgacgcact ggcgcgatgg ccggatcaaa cgggctatgg
    18121 gtattacagt ggtcaacgtt ctgaatatag atacgaccgg tagacgcacg ttcctgcatc
    18181 atcagcgaga acagctcaac ggctttcaca cgctgcttgc ggatgctgtc gtctttctca
    18241 tatttggtat acagacgttc aaactcttcc tgatcggcga agaacgcgtc gtacagcccc
    18301 ggtacgtcgg acgggctgaa cagggtgata tcttcacctt tcagcagacg ggtatacatc
    18361 agtttgttga tttgtacccc gtagtccata tgacgcacgc ggttgccttc cacaccacgg
    18421 ttgtttttca acaccagcag gctttccact tccagatgcc acatcgggta gaacagcgtt
    18481 gccgcaccgc cgcgcacacc gccctgagag caggatttca ccgctgtctg gaaatgtttg
    18541 tagaacggaa tgcagccggt atggaacgct tcaccaccgc gaatcgggct acccagcgca
    18601 cgaatacgcc cggcgttgat gccgatcccg gcacgctggg aaacgtattt aacaatcgcg
    18661 ctggaggtgg cgttgatgga atccaggctg tcaccgcact cgatcagtac gcaggagctg
    18721 aactgacgag tcggggtacg cacgccggac atgattggcg tcggcagcga aattttaaat
    18781 gtggaaaccg cgtcgtaaaa acgcttcaca tattgcaggc gcgtttcacg cgggtagttc
    18841 gagaacaggc acgcggcaac cagaatataa aggaactggg cgctctcata gatttcgccg
    18901 gtcacgcggt tctgtaccag atatttgcct tccagctgct taacggcagc ataagagaag
    18961 gtcatatcac ggtcgtgatc gataaacgtg tccatctgct tgaactcttc ttccgtgtag
    19021 tcttccagca gatgattatc gtatttgccc atctcgacca ttttcaccac gtggtcgtac
    19081 agcgcaggcg gctcaaactg gccgtaggct tttttacgca ggtggaagat cgccaggcgc
    19141 gcggcgagat actgataatc cggcgcatca cgggagatca ggtctgcggc agccttgata
    19201 atggtttcgt ggatgtcaga ggtcttgata ccgtcataaa actgaatgtg ggagcgcagc
    19261 tcgacctggg aaatcgaaac gttatgcagt ccttctgccg cccaatccag aacgcgatgg
    19321 attttgtcga gattgatgcg ctctgtgcta ccgtcgcgct ttgtcaccag cagattctga
    19381 ttcatgtatg tcgtacctgt ttttggaatt ctttccgcaa tagtttcatg cagactacct
    19441 gtagtacggg aagctgctta gaacactata tatagggggt atgtttggga ttcactgcaa
    19501 gatagtgtga aaatggccct cttgcaagtg cataactttg tggataactc aggaaggaaa
    19561 aagtggcttt cgcgcacctt aggtcagaca aggtgtccgg gaaagtcaat gggaagagaa
    19621 aaatttgtta aaaataactg ttcggcgatt tcttgaacgg tagaataggt tggataaatt
    19681 gatatagatg gtttattaat ctttttgcta atgattgcaa aaattgatct aagcacgagt
    19741 catcacataa tctgatgtat cagttgtggt caatatataa gcgaatgtaa gcgattgttg
    19801 atgtcgaaat ggttgttttc tattcggtta ttgttaaatg cgaagccatt ttgttaattt
    19861 accttatatt ataaaaaaat tattcattac tttaatttag tttaaatata actggtatcc
    19921 cttaaggtta ttaacttgaa ttatgacgca ctgatattat tcatcaaata ataacaaaat
    19981 agccattgca tcgggttgga caatttacaa aagaaagata acccatattg tataatgcgt
    20041 ttaaatatgc cgtcttatat aaaatgatgg tcagattaat atgcggatta
//
LOCUS       DAJTNN010000066        20020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTNN010000066 DAJTNN010000000
VERSION     DAJTNN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.066"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..312
                     /locus_tag="OO150_002062"
     CDS             <1..312
                     /locus_tag="OO150_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5516212.1"
                     /translation="CIIVQWDANSNGIWDREPVKESDQIGFRLKEHVLETLRGATSCE
                     GKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVLTVNMRAASKSEPQTVVNASYSVTGFN
                     L"
     gene            309..716
                     /locus_tag="OO150_002063"
     CDS             309..716
                     /locus_tag="OO150_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5516213.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            701..1024
                     /locus_tag="OO150_002064"
     CDS             701..1024
                     /locus_tag="OO150_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5516214.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            1037..4405
                     /gene="recC"
                     /locus_tag="OO150_002065"
     CDS             1037..4405
                     /gene="recC"
                     /locus_tag="OO150_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5516215.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDNI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            4581..7469
                     /gene="ptrA"
                     /locus_tag="OO150_002066"
     CDS             4581..7469
                     /gene="ptrA"
                     /locus_tag="OO150_002066"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5516216.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTTEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            7462..11004
                     /gene="recB"
                     /locus_tag="OO150_002067"
     CDS             7462..11004
                     /gene="recB"
                     /locus_tag="OO150_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5516217.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            11004..12830
                     /gene="recD"
                     /locus_tag="OO150_002068"
     CDS             11004..12830
                     /gene="recD"
                     /locus_tag="OO150_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5516218.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(12892..14223)
                     /gene="argA"
                     /locus_tag="OO150_002069"
     CDS             complement(12892..14223)
                     /gene="argA"
                     /locus_tag="OO150_002069"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5516219.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            14455..15708
                     /gene="amiC"
                     /locus_tag="OO150_002070"
     CDS             14455..15708
                     /gene="amiC"
                     /locus_tag="OO150_002070"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5516220.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15968..16147
                     /locus_tag="OO150_002071"
     CDS             15968..16147
                     /locus_tag="OO150_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR5516221.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKNMVCRSLSILQT
                     GFFMSEMSKKINSTN"
     gene            16144..16791
                     /locus_tag="OO150_002072"
     CDS             16144..16791
                     /locus_tag="OO150_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5516222.1"
                     /translation="MIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNAI
                     SRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILNN
                     YENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEKI
                     KQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDLILEEKLYLSIRIKVLMD"
     gene            16823..18403
                     /locus_tag="OO150_002073"
                     /pseudo
     CDS             16823..18403
                     /locus_tag="OO150_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            18403..19611
                     /locus_tag="OO150_002074"
     CDS             18403..19611
                     /locus_tag="OO150_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HCR5516223.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIQE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNLACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            19580..>20020
                     /locus_tag="OO150_002075"
     CDS             19580..>20020
                     /locus_tag="OO150_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR5516224.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFIRSHHSSLAISCDLSVEIPVKSEA"
BASE COUNT         4958 a         4664 c         5348 g         5050 t
ORIGIN      
        1 tgtatcattg tgcagtggga tgcgaacagt aacggtatct gggatcgcga accggtaaaa
       61 gagtccgacc agattggatt tcgtctgaag gagcatgtgc tggaaacgct acgcggtgcg
      121 acatcctgtg aaggtaaggg ctgggataaa gtcactaatc cggatgccat cattatcgac
      181 acttttcagg tcgtacgtca ggatgtcagc ggcttctcgc cggtgttgac ggttaatatg
      241 cgtgctgcca gtaagtctga accgcaaacc gtggtgaatg ccagctatag cgtgacagga
      301 ttcaacctgt gaaccgcgaa aagggtgttt cgtcactggc tctggtcctg atgctgctgg
      361 ttttgggtag tttgctatta caaggaatga gccaacagga tcgcagtttt gcctctcgcg
      421 tgagcatgga aagtcagtca ttgcgccgcc aggccatcgt tcagtcggcg ctggcgtggg
      481 gaaaaatgca ctcctggcag acgcagccag cagttcagtg ctcgcagtac gctgaaaccg
      541 atgcccaggt ttgtttgcgt ttactggcag ataatgaagc cttattgatt gccggttatg
      601 aaggcgtttc gttgtggcga acaggcgaag tcattgatgg aaacattgtt ttttcgccaa
      661 gcggctggag cgatttttgt ccgctgaaag agagggcgtt atgtcagctt ccctgaagaa
      721 tcaacaaggc tttagcctgc cggaggtaat gttggcgatg gtgttgatgg tgatgattgt
      781 cactgcgtta tcgggtttcc agcgaacatt gatgaacagt cttgccagca gaaaccagta
      841 ccaacagctc tggcggcatg gctggcagca aacgcaactg cgcgcgattt cgccaccagc
      901 taactggcag gtcaaccgat tgcagacatc gcaggcggga tgtgtcagca tcagcgttac
      961 gctagtttca cccgggggca gagaaggcga gatgacccgc ctgcattgcc cgaatcgtca
     1021 gtagtcagga gccgctatgt taagggtcta ccattccaat cgtctggacg tgctggaagc
     1081 gttgatggag tttattgtcg aacgcgaacg gctggacgat cctttcgaac cagagatgat
     1141 tctggtgcaa agtaccggta tggcacagtg gctgcaaatg accctgtcgc aaaagtttgg
     1201 tattgcggca aacattgatt ttccgctgcc tgcgagcttt atctgggata tgttcgtccg
     1261 ggtgttaccg gagatcccca aagagagcgc ctttaacaaa cagagcatga gctggaaact
     1321 gatgactctg ctgccgcaac tgttggagca cgaagacttt accctgttgc ggcattatct
     1381 gactgacgat agcgacaagc gaaaactgtt ccagctttcc tcaaaagcgg cggacctgtt
     1441 tgaccagtat ctggtctatc gcccggagtg gctggcacaa tgggaaacag gacatttggt
     1501 tgaagggctg ggagaagcac aggcctggca agcgccgttg tggaaggcgc tggtggaata
     1561 tacccatgaa ctcggacaac cgcgctggca ccgcgcgaat ctctatcagc gctttatcga
     1621 aacgctggag tccgcgacga cctgcccgcc ggggttacct tcgcgcgtct ttatatgcgg
     1681 tatttccgcg ttaccgcctg tttatctcca ggcgctccag gcgctgggta aacatattga
     1741 aatccatctc ctgtttacca acccctgccg ttattactgg ggcgatatta aagatcctgc
     1801 ttatctggcg aaactgctga ctcgtcagcg tcgacacagt tttgaagatc gcgaattgcc
     1861 gctatttcgc gatagcgaaa atgccggaca gctctttaac agcgatggtg aacaggatgt
     1921 cggcaacccg ctgctggcct catgggggaa gcttgggcgc gactacattt atctcctttc
     1981 tgacctggag agcagccagg agctggacgc ctttgtcgat gtcacgccag ataacctgct
     2041 gcataacatt cagtctgaca ttctggaact ggaaaaccgt gccgttgctg gtgtgaacat
     2101 cgaagagttt tcccgtagcg ataacaaacg cccgcttgat ccactggata gcagtatcac
     2161 cttccacgtt tgccatagcc cgcagcgtga agttgaagtt ttgcacgatc gcctgctggc
     2221 gatgctggaa gaagacccga cacttactcc gcgcgacatc atcgtgatgg tggctgatat
     2281 cgacagctac agtccgttta ttcaggctgt gtttggtagc gcacctgcgg atcgttacct
     2341 gccttacgcc atttccgacc gtcgggcgcg gcagtcgcat ccggtactgg aagcgtttat
     2401 cagcctgtta tcgttgccag acagccgctt tgtgtcggaa gacgtgctgg cattactgga
     2461 tgtgccggtg ctggcggcgc ggtttgacat caccgaagaa gggctgcgtt atttacgcct
     2521 gtgggtcaac gaatccggca ttcgttgggg gatagatgac gacaacgttc gcgagctgga
     2581 actccccgcc accggacaac acacctggcg atttggcctg acgcgtatgt tgctgggcta
     2641 cgcgatggag agcgcgcagg gcgagtggca atcggttcta ccttatgatg agtcgagcgg
     2701 cttaattgca gaactggtgg ggcatctggc ttcactgcta atgcagctaa acatctggcg
     2761 tcgcgggctg gcacaggagc gtccgctaga agagtggttg ccggtttgtc gcgatatgct
     2821 caacgccttc ttcctgccgg atgcggaaac cgaagcggcg atgacgctga tcgaacaaca
     2881 atggcaggcg attatcgccg aaggtttagg ggcgcagtat ggcgacgcgg tgccgctgtc
     2941 actattgcgt gatgaactgg cacagcgtct ggatcaagaa cgtatcagcc agcgttttct
     3001 cgccggaccg gttaacattt gtactctgat gccaatgcgt tcaattccgt tcaaagtggt
     3061 ttgcctgctg ggaatgaacg acggcgttta tccacgtcag cttgcgccat tgggctttga
     3121 cctgatgagc cagaaaccga agcgtggcga ccgtagccgt cgcgatgacg accgctacct
     3181 gttcctggaa gcgttaattt ccgcgcagca aaaactctat atcagttata tcgggctttc
     3241 cattcaggat aacagtgaac gtttcccgtc ggtactggtg caggaactga tcgactacat
     3301 cgggcaaagt cattatctac cgggcgatga agcgctcaac tgtgatgaaa gcgaggcaag
     3361 ggtaaaagcg catcttacct gcctccatac ccggatgccg tttgatccac aaaactacca
     3421 gccaggcgaa cgacaaagct atgctcgtga atggctacct gcggccagcc aggctggtaa
     3481 agcacattct gaatttgttc agccgctgcc gtttacctta ccggaaaccg tgccgctgga
     3541 aacgctacaa cgattctggg cacatccggt gcgggcattt ttccagatgc gtttgcaggt
     3601 gaacttccgt accgaagaca gcgaaatccc cgacaccgag ccatttattc tggaagggct
     3661 tagccgttat caaatcaatc agcagttatt gaatgcactg gttgagcagg atgatgccga
     3721 acgcttgttc cgtcgcttcc gggcggcagg ggatttaccg tatggcgctt ttggtgaaat
     3781 tttctgggaa actcagtgcc aggagatgca gcagcttgcc gacagagtca ttgcctgtcg
     3841 ccagccgggg cagagtatgg aaattgatct cgcctgcaac ggtgtgcaga taactggctg
     3901 gttgccgcag gtgcagccgg atggcctgtt gcgctggcgt ccctctttat taagtgtggc
     3961 gcagggaatg caactttggc tggaacacct tgtctactgt gccagcggtg gtaatggtga
     4021 aagtcgcctt tttctacgca aagacggcga gtggcgtttt ccgccgcttg cagccgaaca
     4081 ggctttgcat tacctctcac aactgattga ggggtatcgt gaagggatgt ccgcgccatt
     4141 gctggtgtta cctgaaagtg gcggcgcgtg gctaaaaacc tgttatgacg cgcaaaacga
     4201 tgccatgctg gatgacgatt ccacgttgca aaaagcccgt acgaaattcc ttcaggctta
     4261 cgaaggcaac atgatggtgc gtggcgaagg tgataatatc tggtatcaaa ggctctggcg
     4321 gcaattaaca ccagagacaa tggaggccat cgttgaacag tcgcaacgtt tcctgttacc
     4381 gctgtttcgc tttaatcagt catgagtgct gtataaaaat tgcgcaatct atccgcttac
     4441 tttatgatgc gcaccagtca cggactgatg gttatataaa cataggctga ctcgtgcagc
     4501 acaagattaa attctggcag atgatttgcg ttaacgtgtt gaatctggac agaaaattaa
     4561 gttgattatg aggtccgtga atgccccgca gcacttggtt caaagcatta ttgttgttag
     4621 ttgccctttg ggcaccctta agtcaggcag aaacgggatg gcagccgatt caggaaacca
     4681 tccgtaaaag tgataaagat aaccgccagt atcaggctat acgtctggat aacggtatgg
     4741 tggtcttgct ggtttctgat ccgcaggcag ttaaatcgct ctcggcgctg gtggtgcccg
     4801 ttgggtcgct ggaagatccc gaggcgtacc aggggctggc acattacctt gaacatatga
     4861 gtctgatggg gtcgaaaaag tacccgcagg ctgacagtct ggccgaatat ctcaaaatgc
     4921 acggcggtag tcacaatgcc agcactgcgc cgtatcgcac ggctttctat ctggaagttg
     4981 agaacgacgc cttgcctggt gcggtagacc gcctggccga tgctattgct gaacctttgc
     5041 tcgacaagaa atatgccgaa cgtgagcgta atgcggtgaa cgctgaatta accatggcgc
     5101 gtacgcgtga cgggatgcgc atggcacagg tcagcgcaga aaccattaac ccggcacacc
     5161 ccggttcaaa gttttctggt ggtaacctcg aaactttaag cgataaacct ggtaatccgg
     5221 tgcagcaggc gctgaaagat ttccacgaga agtactattc cgccaatctg atgaaggcgg
     5281 ttatttacag caataaaccg ctgccggagt tggcaaaaat ggcggcggac acctttggtc
     5341 gcgtgccgaa caaagagagc aaaaaaccgg aaatcaccgt gccggtagtc accgacgcgc
     5401 aaaagggcat tatcattcat tacgtcccgg cactgccgcg taaagttctg cgcgttgagt
     5461 ttcgcatcga taacaactca gcgaagttcc gtagtaaaac ggatgaattg attacctatc
     5521 tgattggtaa tcgcagccca ggtacacttt ccgactggct gcaaaagcag ggattagttg
     5581 agggcattag cgccaactcc gatcctatcg tcaacggcaa cagcggcgta ttagcgatct
     5641 ctgcgtcttt aaccgataaa ggcctggcta atcgcgatca ggttgtggcg gcaattttta
     5701 gctatctcaa tctgttacgt gaaaaaggca ttgataaaca atacttcgat gaactggcga
     5761 atgtgctgga tatcgacttc cgttatccgt cgatcacccg tgatatggat tacgtcgaat
     5821 ggctggcaga taccatgatt cgcgttccgg ttgagcatac gctggatgca gtcaatattg
     5881 ccgatcggta cgatgctaaa gcagtaaaag aacgtctggc gatgatgacg ccgcagaatg
     5941 cgcgtatctg gtatatcagc ccgaaagagc cgcacaataa aacggcttat tttgtcgatg
     6001 cgccgtatca ggtcgataaa atcagcgaac aaactttcgc tgactggcag caaaaagctg
     6061 ccaatattgc gctctcctta ccggagctta acccctatat tcctgacgat ttctcgctga
     6121 ttaagtcaga gaagaaatat gaccatccag agctgattgt tgatgagtcg aatctgcgcg
     6181 tggtgtatgc gccaagccgt tatttttcca gcgaacccaa agctgatgtc agcctgattt
     6241 tgcgtaatcc gaaagccatg gacagcgccc gcaatcaggt gatgtttgcg ctcaatgatt
     6301 atctcgcagg gctggcgctt gatcagttaa gcaaccaggc gtcggttggt ggcataagtt
     6361 tttccaccaa cgctaacaac ggccttatgg ttaatgctaa tggttacacc cagcgtctgc
     6421 cgcagctgtt ccaggcattg ctcgaggggt actttagcta taccgctacg gaagatcagc
     6481 ttgagcaggc gaagtcttgg tataaccaga tgatggattc cgcagaaaag ggtaaagcgt
     6541 ttgagcaggc gattatgccc gcgcagatgc tctcgcaagt gccgtacttc tcgcgagatg
     6601 aacggcgtaa aattttgccc tccattacgt tgaaagaggt gctggcctat cgcgacgcct
     6661 taaaatcagg ggctcgacca gagtttatgg ttatcggcaa catgaccgag gcccaggcaa
     6721 caacgcaggc acgcgatgtg caaaaacagt tgggcgctga tggttcagag tggtgtcgaa
     6781 acaaagatgt agtggtcgat aaaaaacaat ccgtcatctt tgaaaaagcc ggtaacagca
     6841 ccgactccgc actggcagcg gtatttgtac cgactggcta cgatgaatac accagctcag
     6901 cctatagctc tctgttgggg cagatcgtac agccgtggtt ctacaatcag ttgcgtaccg
     6961 aagaacaatt gggctatgcc gtgtttgcgt ttccaatgag cgtggggcgt cagtggggca
     7021 tgggcttcct tttgcaaagc aatgataaac agccttcatt cttgtgggag cgttacaagg
     7081 cgtttttccc aaccacagag gcaaaattgc gagcgatgaa gccagatgag tttgcgcaaa
     7141 tccagcaggc ggtaattacc cagatgctgc aggcaccgca aacgctcggc gaagaagcat
     7201 cgaagttaag taaagatttc gatcgcggca atatgcgctt cgattcgcgt gataaaatcg
     7261 tggcccagat aaaactgctg acgccgcaaa aacttgctga tttcttccat caggcggtgg
     7321 tcgagccgca aggcatggct attctgtcgc agatttccgg cagccagaac gggaaagccg
     7381 aatatgtaca ccctgaaggc tggaaagtgt gggagaacgt cagcgcgttg cagcaaacaa
     7441 tgcccctgat gagtgaaaag aatgagtgat gtcgccgaga cactagatcc tttgcgcttg
     7501 cccttacagg gtgagcgcct gattgaagcc tctgccggca caggcaaaac ctttacgatt
     7561 gcggcgctct atttgcgcct gttacttgga ctaggcggtt ccgccgcctt tccccgcccg
     7621 ctgaccgttg aagaactgct ggtggtcacc tttaccgagg ctgccacggc agaattgcgt
     7681 ggtcgtatcc gtagcaatat ccatgagttg cgcatcgcct gtctgcgtga aactaccgac
     7741 aatccactgt acgaacgtct gctggaagag atcgacgata aagcgcaagc cgcgcagtgg
     7801 ttgttgttag ccgaacggca gatggatgaa gcggcagtct ttactattca cggtttttgc
     7861 cagcgcatgc tcaacctgaa tgcctttgaa tccggcatgc tgtttgagca gcagctgatt
     7921 gaagatgagt ctctgctacg ttaccaggcc tgcgccgatt tctggcgtcg ccactgctac
     7981 ccgctgccgc gtgaaatagc ccaggtcgtc tttgaaacct ggaaagggcc gcaggcgttg
     8041 ctgcgcgata ttaatcgtta tctgcaaggc gaagcgccgg ttatcaaagc accgccgccc
     8101 gatgatgaaa cgctggcttc ccgccacgcg caaattgtgg cgcgtattgc tacggtaaaa
     8161 cagcagtggc gcgacgcagt gggtgaactg gatgcgctga tcgaatcttc tggtattgat
     8221 cgacgcaagt ttaaccgtag caatcaggct aaatggatcg acaagatcag cgcctgggca
     8281 gaagaagaga ccaacagtta tcagttgccg gagtcgctgg aaaaattctc ccagcgtttc
     8341 ttagaagatc gcacgaaagc tgggggggaa accccgcgac atccactgtt tgaggcgatc
     8401 gatcaactgc ttgcagaacc attgtcgatc cgcgatctgg tgatcacccg cgcattggct
     8461 gagatccgcg aaacagtagc gcgtgaaaaa cgccgccgtg gcgaattggg ttttgatgac
     8521 atgttaagtc ggctcgattc cgcgctgcgt agcgaaagcg gtgaggtgtt ggcagcggcg
     8581 atccgtacgc gattcccggt ggcaatgatc gatgaatttc aggataccga cccccagcag
     8641 taccgaattt ttcgccgtat ctggcaccat cagccggaaa ccgcattgtt gctgattggc
     8701 gacccgaagc aggccatata tgcattccgg ggtgcggata tcttcactta tatgaaggcg
     8761 cgtagtgaag ttcacgccca ctacacttta gacaccaact ggcgctccgc acctggaatg
     8821 gtgaacagtg tgaacaagct tttcagccag actgatgacg cgttcatgtt tcgcgaaata
     8881 ccgtttattc cagtgaaatc agccgggaaa aatcaggcgt tacgttttgt atttaaaggt
     8941 gaaacgcagc ctgcgatgaa aatgtggctg atggaaggcg aaagctgcgg cgttggcgac
     9001 tatcaaagta ccatggcgca ggtatgtgct gcgcaaatcc gcgactggct acaagccgga
     9061 cagcggggcg aagcgttgct gatgaacggc gacgacgcgc gtccggtgcg tgcttcggac
     9121 atcagtgtgc tggtgcgcag ccgccaggag gccgcccagg tgcgcgatgc cttaacgttg
     9181 ctggaaatcc cttccgttta cctttcgaac cgcgacagtg tttttgaaac tctggaagcg
     9241 caggaaatgc tttggttatt gcaggcggtg atgacgcccg aacgtgagaa caccctgcgt
     9301 agtgcgctgg caacgtcaat gatggggctg aacgcgctgg atatcgaaac gctgaataat
     9361 gacgaacatg cgtgggatgc ggtagttgaa gagttcgatg gttatcggca aatctggcgc
     9421 aaacgtggcg ttatgccgat gctgcgggcg ctgatgtcgg cgcgtaacat tgcagaaaac
     9481 ttgctggcaa cggcaggcgg tgagcggcgt cttaccgata tcttgcatat cagcgaactg
     9541 ctacaagaag ccggaacgca gctggaaagt gaacatgcgc tggtacgctg gttatcgcaa
     9601 catatcctcg agccagacag taatgcctcc agccaacaaa tgcgcctcga aagtgataaa
     9661 catctggtgc agattgtcac gatccataaa tcgaaagggc tggaatatcc gctggtctgg
     9721 ttgccattta ttaccaattt ccgcgtccag gatcaggcgt tttatcacga tcgccactcg
     9781 tttgaggcag ttttggatct taatgctgcg ccaaaaagcg tcgacctcgc ggaggtcgaa
     9841 cgtctggcgg aagatctgcg tttgctttac gtggcgctga cgcgttcggt ttggcattgc
     9901 agtctcggcg ttgcaccgct ggtgcgccgt cgtggcgata aaaaaggtga caccgacgtc
     9961 caccaaagtg cgctcgggcg tttgctgcaa aaaggggaac cgcaagatgc ggcagggctt
    10021 cgcacctgta ttgaagcgtt atgcgatgat gatattgcct ggcaaacggc acaaactggt
    10081 gataaccagc cctggcaggt taatgatgct ttaactgcag aactgaatgc gaggacacta
    10141 caacgattgc ccggcgataa ctggcgtgtc accagctact ccggcttgca gcagcgtggt
    10201 cacggtatcg ctcaggatct gatgccacgg ctggatgttg atgccgcagg cgtggtcagc
    10261 gtcgttgaag aaccgacgtt aacaccgcat cagttcccgc gcggtgcgtc accggggaca
    10321 ttcttgcaca gtttgtttga agacctcgat tttacccagc cggttgaccc gaactgggta
    10381 caggaaaaac tggaactcgg cggctttgaa tcgcagtggg aaccggtgtt gaccgagtgg
    10441 atcacggctg tcctccaggc acctctcaat gaaaaggggg ttagcctgag tcagctttcc
    10501 gaccgcgata aacaggtgga gatggagttt tacctgccga ttagcgaacc gctcatcgct
    10561 agccagcttg atgcactaat tcgccagttt gatccgctat ccgctggctg cccgccgctg
    10621 gagttcatgc aggtacgcgg catgttaaaa ggctttatcg acctagtatt ccgccacgaa
    10681 gggcgttatt acctgctcga ctataaatcc aactggctgg gtgaagacag ttcggcttac
    10741 acccaacagg ctatggcagc agcgatgcag gcacaccgct atgatctgca atatcagctt
    10801 tataccctgg cgctgcatcg ttatctgcgc catcgcattg ctgattacga ctatgatcgt
    10861 cactttggcg gcgttatcta tctgttcctg cgtggcgttg ataaagaaca tccgcaacaa
    10921 gggatttaca caacccgacc caacgccggg ttgattgccc tgatggatga gatgtttgcc
    10981 ggtatgaccc tggaggaggc gtaatgaaat tgcaaaagca attactggaa gctgtggagc
    11041 acaaacagct acgcccgctg gatgtgcaat ttgccctgac cgtggcggga gatgaacatc
    11101 ctgccgtcac cctcgcggcg gcactgttaa gtcatgatgc cggagaggga cacgtctgtt
    11161 tgccgctttc acgactggaa aataacgagg cgtcgcatcc gctgttggcg acctgtgtca
    11221 gtgaaatcgg tgagctacaa aattgggaag aatgcttgct ggcttctcaa gcggtcagca
    11281 ggggagatga acccacgccg atgatcctct gtggcgatcg tctttatttg aatcgcatgt
    11341 ggtgtaacga gcggacggta gcgcgcttct tcaatgaggt gaatcatgcc attgaggttg
    11401 atgaagctct actggcgcaa accctggaca aactttttcc agtaagcgat gaaattaact
    11461 ggcaaaaagt tgcagcggca gtggcgctga cgcggcggat ctcggtgatt tccggcggcc
    11521 caggcaccgg taaaacgacc accgtggcga agttgctggc agcgttaatt caaatggccg
    11581 acggcgaacg ctgccgtatc cgtctggctg caccaacggg taaagctgcc gcgcgcttaa
    11641 ccgaatctct cggcaaggct ttgcgacagt taccgctgac cgatgaacaa aagaaacgca
    11701 ttccggaaga tgccagcact ttgcaccgat tgctgggcgc gcagccgggt agccagcgtt
    11761 tacgtcatca tgccggtaac ccgctgcatc ttgatgtgct ggtggtagat gaagcgtcaa
    11821 tgatcgatct gcctatgatg tcgagactga tcgacgcctt gcccgatcat gcgcgagtga
    11881 tctttctcgg cgatcgtgat caactggcct cggttgaggc tggggctgtg ctgggcgata
    11941 tctgcgctta tgccaacgcg ggctttaccg ccgagcgtgc caggcagcta agccgcctga
    12001 cggggactca cgttccggca ggaaccggca cagaagcggc atctttgcgc gacagtctct
    12061 gcctgctgca aaaaagctat cgtttcggca gcgattctgg cattggtcag ttagctgcgg
    12121 cgatcaaccg tggtgataaa acggcagtga aaaccgtttt tcagcaggat tttactgata
    12181 tcgaaaaacg acttttacag agcggcgaag attatattgc gatgcttgag gaagctcttg
    12241 cgggttatgg acgttatctg gatctgctgc aagcgcgtgc cgagccggat ttaatcattc
    12301 aggcgttcaa tgagtaccaa cttttgtgcg ccctgcggga agggccgttt ggcgtggctg
    12361 gactgaatga gcgaattgag cagtttatgc aacagaagcg caaaattcat cgtcatccgc
    12421 actctcgttg gtacgaaggt cgaccggtga tgattgcccg taatgacagc gcgcttgggt
    12481 tgtttaatgg cgatatcggt attgcgctgg atcgcgggca ggggacgcgc gtctggtttg
    12541 cgatgccgga cggcaatatt aagtctgtgc aaccgagtcg cttgccagag cacgaaacta
    12601 cgtgggcgat gacggtacat aaatcgcagg gatcggagtt cgaccatgcg gcgttgattt
    12661 taccgagcca acgcacgccg gtagtaacgc gagagctggt atacaccgcg gtgacccgcg
    12721 cgcgtcgccg tctgtcgctg tatgccgatg agcgtatatt aagtgcggca atcgccactc
    12781 gtactgagcg gcgcagtggt ctggcggcgt tgtttagttc gcgggaataa acgtaattgc
    12841 cggatgcgac gcacgagtgt tacgcatgtc gcatccgaca attttcatcg cttaccctaa
    12901 atccgccatc aacactttgg atttacgctg gtagttgtac aactgctttt tgctctcggg
    12961 cagtaaatca atatccaccg gggtaaatcc acgttcctgg aaccagtgaa tactgcgcgt
    13021 ggtcagcaca aacaatttgc ttaagccgct ctgcttcgcc tgagcggcaa tgcgttccag
    13081 cagaacctcg ccccgtgatg aactgcggta atccgggtga actgccacac aggccatttc
    13141 cccaatcttc tcttccggga acggatagag cgcggcgcag gcaatagtcg tgttatcgcg
    13201 ctgaataatg gtgaatttgt cgatttccat ctccagctgc tcgcgagaac ggcgcaccag
    13261 aataccttgc tgctccagcg ggcgaatcaa ctccagaata ccgccaatat cgttgattgt
    13321 tgcgcgacga atctgctcgg cgctttccat cacaatctgc gtaccgatac cgtcgcgtga
    13381 gaacaactct tgcaacagcg cgccatcttc ctgataactg attaaatgac agcgacgcac
    13441 gccgctgcgg caggctttca ctgcgccacg caaaaagcgc accgtaccgg agttgtaatc
    13501 gcctttctct tcctgggctt ctacccgcgc ttgcgcttcg ttagggaaaa gttctgagac
    13561 aatatcaccg tcgtcattag tgacgccctg tgaagagcaa aaaccaatca tcttctcagc
    13621 tttcagtttg atggccagtt gagtggcaat ctcttccgag gtcagattaa agctctcgcc
    13681 agtgactgaa acagcgaccg gccccattag cactattgca ccgctgtcca gttgacgatg
    13741 gatcgcgtct tcatcaatcc gccggatacg cccgctatgg cagtaatcca cgccgtcatc
    13801 gacgcccagc ggctgggcaa taataaaatt gccactgacg acgttgatat gcgcgccctg
    13861 tagcggcgtg ttattgagac tcatcgacag gcgagcagtg atgtccagtt gcaatgttcc
    13921 cgcagcctgc ttcaccagtt ccagtgtttt ggcgtcggtc acacgtatat tcttgtgata
    13981 cagcggttcg tggtgatgcg cggccagatt tgcgtcgatc tgcggacgtg cgccatagac
    14041 caccaccaga cggatgccga ggctgtgcaa caacccgata tcattaacga tactggagaa
    14101 attctcatgc tcaatggctt caccgccgag catgatgaca aacgtttttc cccggtgggt
    14161 attgatatag ggaaccgaat ggcggaatcc ctcgaccagc tcggttttac gttcctttac
    14221 cacggcacac ccctttgcat gattattcta aattagtgta tttttattct gttttttcgc
    14281 agggtgcaag tgtaaatttt atgcgagagc gactttttta tcagtaatgc cgtgaatata
    14341 aaaagaatgt ttaccgttta ttagatgaca gattatgcgt ctttcgctaa agtttccggt
    14401 caaattagtc gtttacttgt tacacagctt agatttcttt tgctcgggag aggcatgtca
    14461 ggatccaaca ctgcaatcag ccgtcgtcgt ttactgcaag gcgcgggtgc catgtggcta
    14521 ttgagcgtaa gtcaggtcag tctggctgcg gtcagccagg tcgtggcggt gcgcgtctgg
    14581 cctgcgtcca gctacacccg tgtgacggta gaatcaaatc gtcagctgaa atataagcag
    14641 ttcgcgttga gtaaccctga acgcgtggtg gtggatatcg aagatgtaaa cctgaactcg
    14701 gtactcaagg ggatggcggc acaaattcgt gcagacgacc cgttcatcaa gtcggcgcgc
    14761 gtcgggcaat ttgacccgca gaccgtacgg atggtttttg aattaaagca aaacgtaaaa
    14821 ccgcagctgt ttgcccttgc gccggtcgcc gggtttaaag agcgtctggt gatggacctc
    14881 tatccggcca atgcacagga tatgcaggac ccgctgctgg cgctgctgga ggattacaac
    14941 aaaggcgacc tcgaaaagca ggtgccgcca gcacaaagtg gtccacaacc gggtaaagca
    15001 gggcgcgatc gtcctattgt tattatgctt gaccctggcc acggcggcga agactccggt
    15061 gcggtgggga aatacaaaac gcgcgaaaaa gacgtggtat tgcaaatagc tcgccgtctg
    15121 cgctctctga tcgagaaaga gggcaatatg aaggtgtaca tgacgcgcaa tgaagacatc
    15181 ttcattccgt tgcaagtgcg cgtagcaaaa gcccagaaac agcgtgctga cctgtttgtc
    15241 tctatccatg ccgacgcctt taccagtcgt cagccgagcg gttcctctgt gtttgcgctc
    15301 tcaaccaaag gtgcaaccag tactgcggca aaatatctgg cacaaaccca gaacgcctcg
    15361 gacttgattg gtggcgtgag caaaagcggt gaccgctatg tcgaccacac catgttcgat
    15421 atggtgcagt cgctgaccat tgccgacagc ctgaagtttg gtaaagcggt actgaataag
    15481 ctcggtaaaa tcaacaagct gcataaaaat caagttgaac aggccgggtt tgccgtacta
    15541 aaggcaccag atattccctc cattctggtc gaaacggcgt ttattagtaa cgttgaggaa
    15601 gagcgtaaac tgaaaacggc gactttccag caggaagttg cggagtctat tcttgcgggg
    15661 attaaagcgt attttgccga tggggcgacg ctggcgagaa ggggatgatg tttttaattt
    15721 attcaatgaa tttatgtttc attgaataaa tattatttac ataaatgttg aacgatggtt
    15781 ttgtgactgc tgtcgaatat ttgaatatcg gcttaaaaaa taagtctgtc atctctttta
    15841 ttaagcacaa ttccgtgtaa ggtatacgca ttagttaatt atcttgcaac tgttaaatca
    15901 ggcaaggcaa tgtttgaagt aattattact tctgacatgc cttgcctttt ttttggggag
    15961 ccatggaatg atcatcgaaa aagtcatgaa caataattgt gtacaggcat cggtgaacgg
    16021 acaggaggtt atcatttctg ggcctggcgt tggttacaat aaaaatatgg tatgtcggtc
    16081 cctgagcatc ctgcaaaccg gattttttat gtcagaaatg agcaaaaaaa taaactctac
    16141 aaattgattg agcatgtggg tattgagtat atttttgttg ccgaaaagat agtgcattat
    16201 gcggagagta atctcgaaaa aaaactcaat ccatcgctac tactgattct tgctgatcac
    16261 atttcgaatg caatatcccg agtcgtctca ggcatacaaa ttaataatgt tttccttgag
    16321 gaaatcaaag cgttgtacaa agcagagtat gcaataagtc gtgatgcatt aacaatcatt
    16381 aatgagcaat ttagcgttca actaccggat gatgagattg gttttatagc attgcatatt
    16441 ttaaataatt atgaaaattc agttgattat gaatcagtaa ggattattga gctgtctcaa
    16501 aaaatcacgg agcttattga agttgtttat aacagaaggg tggacagaag ttcatttaac
    16561 tactccagat ttatgatgca ccttaaatat ttttctagtc gtgtgttatg taatgaaaaa
    16621 ataaaacaga aaaatattgg tgatatctat gagcagtttc ttgaaaaaga ttttcagtta
    16681 caacgtgcca ttcatgaaat tgaaagatat ctgtacgcaa cttttaaata tgatttgatt
    16741 ctggaagaga aactatatct ttctattcgc atcaaagtat taatggacta attatacata
    16801 acattttcat gagataacca gtatgaaaca aaagaaatcc tggagttttt ttcagagcct
    16861 ggggaaggca tttatgtatc ccatcgctct gttaagtgta tgtggtatga tgttagggct
    16921 gggaagtggt ttagccagcg atgatatggc aaaattcatt ccagtgttgg ctattccgtt
    16981 aattaaaacc atacttgatt ttattgttag ccttggtttg tttgccttcg ttaatttacc
    17041 tgtattgttt gcgatagcta tacctttggg attattaaaa gagaaagatg ataaagccta
    17101 tggcgctttt tctggcttaa ttggctttat ggcgatgcat cttggaacga acttttatct
    17161 taaacagcac gacttattgg tagctgctga ccaaatgtcg acacatgggc aaaccatcat
    17221 actcgggatc cagtcctaca ataccagcgt gttgggtggg attgtggcag ggttattagt
    17281 cgccagcatg tataaaaaga tcgttaactt acgtattcct gaataattag ggttttatag
    17341 tggcccacgc cttgtgccta tcattacact gattgtgatg agtggatttg gtctgatcat
    17401 tccttttatc tggccgccat ttttcaatct tttcatgctc attggtcact ggatttcaac
    17461 ttccggtcct gttggttatt tcttctatgc tgttgccgaa cgcgtgacga taccttttgg
    17521 cttaaatcac ttggtgacat cagttttccg ctttacccca atcggcggat cggcagtgat
    17581 tggcggtgaa gagtattacg gcaccctgaa catgtttatg gcatacgtca aagagaatgc
    17641 ggtcattccg ctggatttgg cggggaaaat ggagcagggc aaactgatga ttcagtatgg
    17701 tctggctgga gccgcgctgg cgatgtatcg cactgctcat gctcaaaaca gaagggctat
    17761 caaagcattg ctgatttccg gtgtgctcac ggtaattatt ggcggcgtca gcgagccgat
    17821 tgaatttctg ttcttatttg tcagcccgct gttgtttgtc ttccatgcct ttatgaatgg
    17881 attggctaac atggttctgc catatatggg cgtgaagatg ggctttactg gcgatctgat
    17941 tcaatttatt agctttggtg tcttacgtgg cacaagaacg ggatggccat tagcggtatg
    18001 tgttgaagtg gcttatttct tcatttatta ctttgtgttc cgttggacca ttctcaaatt
    18061 taacctgatg actgtaggcc gtgaagagtc cagtcctgtc acgctgaacg ctcacgaaga
    18121 aactgcaata gctaacatcc cagttcatga taaatcagag ttgcaggcag cggagcaaat
    18181 ggttaaggct ctgggtggta aagaaaatat taaatcactg gataattgtg taactcgttt
    18241 gcgtttaact atcgcggata tgggattgat aaatgaggct gcaataaaaa gggccggagg
    18301 aattgcggtt gtgaaacttg atcaaaatac cttacaagtc attatcggta caaaagtcat
    18361 cgccctacgt cgggatatgg ataattacat ggggatatgt tgatggatat ttttaatacc
    18421 tcaatttctc gtaaaggaac atattgcaca caatgggact tttgtgaaga cagatttggt
    18481 gtaaaagatg tattaccatt ctctatttca gatatggatc ttccgataca ggaagctatc
    18541 atcagaacat taaaaaaacg tctggaacac ccgattttag ggtacagtcg ttggcagcat
    18601 gatgactatc tgaattcgat agtcaattgg tatcatcaac aatataaaac agagattcaa
    18661 ccagaatgga tcacctatag cccaagcgtg atgtattcaa tatctaaggc aatagaaata
    18721 ttgacattga atggagataa tattctcatt ttcacgcctg tttataacgc attttttgat
    18781 gtgattaagc ataatggtcg aaacatgcta acaacctctt tgatgaaaga aaatgggaga
    18841 ggctacgtta tcaactggca agactttgag cttaaaatta aaagcgcgaa gatggtgtta
    18901 ttatgtaatc ctcataaccc gacaggaacg gtctggtcgg aagacgatct tcacagaatc
    18961 gcagcatgct gtgcaaggca taatatttgg ttatgttcag atgaaataca tagtgatttt
    19021 gtttttaatc atagtttcac gtcagcgcta acaataaaag atgacaaagt ggtggtgttt
    19081 aattcgattt cgaaaacatt taatgttcct gcgctaaccg gatcatatat gatttcaact
    19141 gacgataatt ttaatcataa gttcagaacg atatcgaggt atcgcgattt cgtaaactcc
    19201 ccatcagtac tgaatgtaat tgcgaccatt gttgcctata atgaatgtga agaatggtta
    19261 aagtcattaa aggtacatat tgcctctaac attcaattta ccttgcagta tttaaatgaa
    19321 aatattccgg aattgatcgt atggccagct gagggctgct atttttcgtg ggttgattgc
    19381 tcggcccttg gttatccctt tgataaattt taccgtcgat taattaacga agggagagta
    19441 ggtattatgg cggggcatgt ttatggtact gaaggagagg gctatctgcg tcttaatctg
    19501 gcctgcagtc gagaaaaatt atatatgggg cttgcgcgcc ttgcgagtgt aattaaaaat
    19561 attaatcagg aaaaagaaaa tgaacaatac ggatcttatc catcttatta aacgttttat
    19621 gcgtaacgaa cataaagcag tagaagaggt tattgattcc cccctttcgg aagtcgcgaa
    19681 tttaattaaa atattacaat cttgccaggg aaaagttgtt tttattggtg ttgggaaatc
    19741 tggcattatt gccagaaaac ttgctgcaac atttgccagc accggaactc cctcgttttt
    19801 tgttcacggt acggaagcgg tacacggcga ccttgggatg gtggcgaaag acgatgttgt
    19861 tattcttatt tcaaacagtg gtgagacggc ggaaatcctg gcgacactgc ctagtttgaa
    19921 aaaaatgggt aattatttga tctcttttat ccgcagtcat cactcatcgc ttgcgataag
    19981 ttgtgattta tctgttgaaa tccccgtcaa aagtgaggcg
//
LOCUS       DAJTNN010000067        19768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTNN010000067 DAJTNN010000000
VERSION     DAJTNN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.067"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..723
                     /gene="eutC"
                     /locus_tag="OO150_002076"
     CDS             <1..723
                     /gene="eutC"
                     /locus_tag="OO150_002076"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR5516225.1"
                     /translation="IGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADH
                     SRSKDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVA
                     NPDVQVVISDGLSTDAITVNYEELLPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQICE
                     ILGAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAA
                     AVIVDLAKRMLEQKASGINMTR"
     gene            733..1392
                     /gene="eutL"
                     /locus_tag="OO150_002077"
     CDS             733..1392
                     /gene="eutL"
                     /locus_tag="OO150_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5516226.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1405..1905
                     /gene="eutK"
                     /locus_tag="OO150_002078"
     CDS             1405..1905
                     /gene="eutK"
                     /locus_tag="OO150_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5516227.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAAGRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            1951..3003
                     /gene="eutR"
                     /locus_tag="OO150_002079"
     CDS             1951..3003
                     /gene="eutR"
                     /locus_tag="OO150_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR5516228.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(3009..3908)
                     /gene="hemF"
                     /locus_tag="OO150_002080"
     CDS             complement(3009..3908)
                     /gene="hemF"
                     /locus_tag="OO150_002080"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5516229.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCNDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(3912..4781)
                     /gene="amiA"
                     /locus_tag="OO150_002081"
     CDS             complement(3912..4781)
                     /gene="amiA"
                     /locus_tag="OO150_002081"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5516230.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            4995..5420
                     /locus_tag="OO150_002082"
     CDS             4995..5420
                     /locus_tag="OO150_002082"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5516231.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5407..5856
                     /locus_tag="OO150_002083"
     CDS             5407..5856
                     /locus_tag="OO150_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5516232.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6063..6638
                     /locus_tag="OO150_002084"
     CDS             6063..6638
                     /locus_tag="OO150_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5516233.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFRGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6734..7633
                     /gene="yfeX"
                     /locus_tag="OO150_002085"
     CDS             6734..7633
                     /gene="yfeX"
                     /locus_tag="OO150_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5516234.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(7691..8995)
                     /gene="pbp4b"
                     /locus_tag="OO150_002086"
                     /pseudo
     CDS             complement(7691..8995)
                     /gene="pbp4b"
                     /locus_tag="OO150_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(9000..10424)
                     /gene="murP"
                     /locus_tag="OO150_002087"
     CDS             complement(9000..10424)
                     /gene="murP"
                     /locus_tag="OO150_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5516235.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(10428..11324)
                     /gene="murQ"
                     /locus_tag="OO150_002088"
     CDS             complement(10428..11324)
                     /gene="murQ"
                     /locus_tag="OO150_002088"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5516236.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            11488..12345
                     /gene="murR"
                     /locus_tag="OO150_002089"
     CDS             11488..12345
                     /gene="murR"
                     /locus_tag="OO150_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5516237.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCVEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            12474..13265
                     /gene="ucpA"
                     /locus_tag="OO150_002090"
     CDS             12474..13265
                     /gene="ucpA"
                     /locus_tag="OO150_002090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5516238.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            13569..14585
                     /gene="cysP"
                     /locus_tag="OO150_002091"
     CDS             13569..14585
                     /gene="cysP"
                     /locus_tag="OO150_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5516239.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            14585..15418
                     /gene="cysT"
                     /locus_tag="OO150_002092"
     CDS             14585..15418
                     /gene="cysT"
                     /locus_tag="OO150_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5516240.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            15418..16293
                     /gene="cysW"
                     /locus_tag="OO150_002093"
     CDS             15418..16293
                     /gene="cysW"
                     /locus_tag="OO150_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5516241.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            16283..17380
                     /gene="cysA"
                     /locus_tag="OO150_002094"
     CDS             16283..17380
                     /gene="cysA"
                     /locus_tag="OO150_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5516242.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            17514..18425
                     /gene="cysM"
                     /locus_tag="OO150_002095"
     CDS             17514..18425
                     /gene="cysM"
                     /locus_tag="OO150_002095"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5516243.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18428..18796)
                     /locus_tag="OO150_002096"
     CDS             complement(18428..18796)
                     /locus_tag="OO150_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5516244.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            18901..19752
                     /gene="pdxK"
                     /locus_tag="OO150_002097"
                     /gene_synonym="thiJ"
     CDS             18901..19752
                     /gene="pdxK"
                     /locus_tag="OO150_002097"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5516245.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         4548 a         5010 c         5576 g         4634 t
ORIGIN      
        1 attggtgttg aaaacccgca tcgcgcagac gtattaacag aactgcgtcg cagcaccgtg
       61 gcccgcgttt gtaccggtcg tgccggtccg cgtccgcgta cccaggcgtt gctgcgtttc
      121 ctggccgatc actctcgttc gaaagatacc gtactgaaag aagtgccgga agagtgggtg
      181 aaagcgcagg gcttgctgga agtacgctcc gaaattagcg acaaaaatct ctacctgact
      241 cgcccggata tgggccgccg cctgtgtgca gaagctgttg aagcgctgaa agcacagtgt
      301 gttgctaatc cggacgtaca ggtcgttatt tctgatggcc tgtcaactga tgccatcacc
      361 gtgaactacg aagagctcct gccgccgctg atggcgggcc tgaaacaggc cgggctgaaa
      421 gtcggcacgc cattctttgt tcgttatggt cgcgtgaaga ttgaagatca gatctgtgag
      481 atcctcggcg cgaaagtggt gatcctgctg gtgggggaac gtccggggtt gggtcagtca
      541 gaaagcctct cctgttacgc cgtttactcg ccgcgtatgg cgaccaccgt tgaggccgat
      601 cgcacctgca tctctaacat tcaccagggc ggcacgccgc cggttgaagc cgcagccgtc
      661 attgtggatt tggcgaaacg tatgctggag cagaaagcat ccggcatcaa catgacccga
      721 taaggaggca tcatgccagc tttagatttg attcgaccgt cggtaaccgc catgcgggtg
      781 attgcctccg ttaacgctga atttgcgcgt gaactaaaat tgccgccgca tattcgtagc
      841 ctcgggctga tttctgctga ttccgatgac gtcacctaca ttgcggctga cgaagcgacc
      901 aagcaggcga tggtcgaagt ggtatatggc cgctcgctgt atgcgggagc agcacacgga
      961 ccgtcaccga ctgccggtga agtgctgatt atgcttggtg ggccaaaccc ggcggaagtg
     1021 cgtgctggtc tggatgcgat ggttgcgcat attgaaaatg gcgctgcttt ccagtgggct
     1081 aatgacgcag aaaacaccgc attcctggca catgtagttt cgcgtaccgg ttcttatctc
     1141 tcatcaaccg ccgggatcac gcttggcgat ccgatggcgt atctggtggc accgccgctg
     1201 gaagcgacct acggcattga tgcagcgttg aaatctgccg acgtacagct ggtgacctat
     1261 gtcccgccac cgtctgaaac caactactcg gcagcatttt taaccggtag ccaggccgcg
     1321 tgtaaagcag cctgtaacgc ctttaccgat gcagtgctgg aaatcgcgcg taatccaatc
     1381 cagcgtgcgt aacggaggtt gccgatgatc aatgcactgg gattgctgga agtggacggc
     1441 atggtcgccg cgatagatgc agcggatgcc atgctcaaag cagctaacgt tcgtctgctc
     1501 agtcacgaag tgcttgaccc tggtcgctta acgctggtgg tggaaggcga tctggcggcg
     1561 ggtcgtgcgg cgctggacgc aggttgtgct gccgcgatgc gtaccgggcg tgtcatcagc
     1621 cgcaaggaga tcggtcggcc agacgatgac acccagtggc tggttactgg ctttaaccgc
     1681 cagccgaagc aacccgtaag ggaacccgac gcgccagtta tcgtcgcgga atctgctgac
     1741 gagttgttgg cgctgttaac atcagtacgt cagggaatga cggcaggaga agtggctgcc
     1801 cactttggct ggccgctgga aaaagccaga aatgcgctcg aacagctctt ttctgccggg
     1861 acgttacgta aacgcagtag tcgttatcgt ctcaagcccc attaacctgt cggaggtgct
     1921 gggtgtccta caacacccgg cattaacatc atgaaaaaga cccgtacagc caatttgcac
     1981 catctttatc atgaaccctt acccgaaaac ctgaagctca cgccgaaggt cgaagtggat
     2041 aatgttcatc aacgacagac aacggatgtc tatgaacatg ctttaacgat taccgcctgg
     2101 cagcagattt acgatcagct gcatccgggc aagtttcatg gtgaatttac ggaaattcta
     2161 ctcgatgata ttcaggtttt tcgtgaatac accggtctgg cgctgcgtca gtcgtgcctg
     2221 gtctggccga actcgttctg gtttggcatt ccggcgacgc gcggtgagca gggatttatc
     2281 ggttcgcaat gtctgggaag cgcggaaatc gccacccgcc ctggtggcac tgaatttgaa
     2341 ctgagcacgc cggatgatta cacgatcctg ggcgtggtgc tttctgaaga tgtcatcacc
     2401 cggcaggcta actttttgca taacccggat cgggtattac atatgctgcg tagccagtcg
     2461 gcgctggaag tgaaagagca gcataaagcc gcgctgtggg gctttgtcca acaggcgctg
     2521 gcgacgtttt gcgagaaccc ggaaaatctc catcagcctg cagtgcgaaa agtgctgggg
     2581 gataatttgc taatggcgat gggggcgatg ctggaagacg cgcaaccaat ggtgacggcg
     2641 gaaagcatca gtcatcagag ttaccgtaga ttactttccc gcgcccgtga atatgtgctg
     2701 gaaaatatgt ctgagccggt gacggtgctg gacttgtgta atcaactgca tgtcagtcgc
     2761 cgcacgctac aaaacgcgtt tcacgctatt ttaggcattg gcccaaacgc gtggctgaaa
     2821 cgcattcgcc tgaacgccgt acgccgcgaa ctgataagcc cgtggtcgca aagcacaacg
     2881 gtaaaagacg ccgccatgca gtggggattc tggcatctgg ggcaatttgt cacggattac
     2941 cagcagctgt ttgccgagaa gccgtcgttg acgttgcatc agcggatgcg ggaatggggg
     3001 tgagggagtt acacccaatc cctgacctta ataaactcac ttaacgccgc ttctgggctg
     3061 ccatcttttg gctgataatc atactcccag cgtaccagtg gcggcattga catcaggatc
     3121 gactcggtgc gcccgccagt ttgcaggcca aacagagtgc cgcgatccca gaccagattg
     3181 aactcgacat aacgaccgcg acggtagagc tgaaaattgc gctcgcgctc gccgtaggcc
     3241 attgctttac gtcgctctac aattggtaaa taagcgtcgg tgtagccttt gcctaccgcc
     3301 tgcataaagg caaaacagtg gtcgaaatct ggcgtgttca gatcatcaaa gaacagcccg
     3361 ccaataccgc gctgttcgtt gcgatgtttg aggtagaagt aatcgttgca ccactttttg
     3421 taacggggat aaacgtcttc accaaatggc aggcacaggt cacgggcggt gcggtgccag
     3481 tgaatggcgt cttcttcaaa accatagaaa ggggttaaat cgaagccgcc gccaaaccac
     3541 cagacgggct ctgcacccgg tttttcggca ataaaaaaac gcacattcgc gtggctggtg
     3601 ggaacatacg ggttatgcgg atgcactacc agtgaaacgc ccatcgcctc gaaactgcgc
     3661 ccggcaagtt ccgggcgatg agcggtggcg gaagcaggca tcgcctcgcc gtggacgtga
     3721 gaaaagttga cgcctgcctg ttcgaaaaca ccaccattac gcaacacccg actacgcccg
     3781 ccgccgccag cttcgcgctg ccaactatct tcgacaaatt ctgcgccatc gacggcggtc
     3841 agctgctgac aaatcgtatc ctgaaggttg agcagaaact gtttaacctg gtgtgcgtcg
     3901 ggtttcataa cttatcgctt tttcgaatgt gctttctggt tgtcgaacca gtggaaataa
     3961 ctgatcacgc cttcagcaat cgctgtggcg attttctgac gaaacgccgc cgtgcctaac
     4021 agccgctctt cttccgggtt ggtgataaac gaggtttcca ccagcaccga aggaaccgac
     4081 ggcgatttca acaccacaaa cgcggcttgt tcggtgttgc ggctgtgcag tttatgcacc
     4141 ggcttaatct ttttcagaat atgcgagcca agcgtcaggc tgtttttaat agtatcggtt
     4201 tgcaccagat caaacagcac ttgctgcaat aggtgatcct tgtcagtcgc ctttttaccg
     4261 gcaacttcat cggcgcggtt ttcgcgttca gacaggtatt tcgccattgc gctacttgcc
     4321 ccacggttag agagggcaaa taccgaagca ccggcagctt tcgggttggt aaagccatcg
     4381 gcatgaattg acataaacag atctgcgcca tgtttatggg cgatttcaac gcgatcgtaa
     4441 agtgggataa acgtatcgcc agaacgcgtt aaacgcgcat caatcccatg attacgcaaa
     4501 atggaacgga cgtttttagc aatcgccagc accacatgtt tttctttcga accgttgcgt
     4561 ccgatcgctc cggtatcaat tccgccgtgg cctggatcga gaacaacgac acgtttgccg
     4621 ccagattttt tggctttcgg cttgctgtgt ccgttgctgg tttttaaagg ttcgtctttg
     4681 gcgatggctt gcgacattcc tgacaacgtc agggcagcca aaccggcttt cagcacctgg
     4741 cggcgcgaag tgagtgtttt tagtggttta aaagtgctca tacggcctga gttgtaataa
     4801 taaagttcca gatgttatat cgtatcgcgt aatccgttac gacggtttga tgttgagaat
     4861 tgttttactt ttcatttcaa tagttgacaa ctccccatta tgccactttt tcaaccgatt
     4921 ttcgtcagat attggctaat tctaccgttc acgccataat cagcctttta acccgcaaaa
     4981 aggtaacgaa taccatggag atacgcgtat ttcgccagga agatttcgaa gaggtcatca
     5041 ccctttggga gcgttgcgac ttgctgcgtc cgtggaacga tccggaaatg gacatcgagc
     5101 gtaagatgaa ccatgacgtc agtttgtttt tggtcgctga ggtgaacggt gaagtggtcg
     5161 gaacggtgat gggcggttat gacgggcatc gcgggtctgc ttattatctt ggcgtgcatc
     5221 cagagtttcg tgggcgtggg attgccaatg cgttgcttaa tcggctggag aaaaagctga
     5281 ttgctcgtgg ctgcccgaaa attcagatca atgtgccgga agataacgac atggtgctcg
     5341 gaatgtatga acgcctggga tatgaacacg ccgacatgct gagtctgggt aagcgtttga
     5401 ttgaagatga agagtactga gttccatcct gtccattatg atgcgcacgg acgcctgcgt
     5461 ttacccttgc tcttctggct tgtgctattg cttcaggcgc gaacctgggt gctgtttatc
     5521 attgccggtg cgtcgcgcga gcagggtact gcgctgctaa atctgtttta tcccgatcac
     5581 gataatttct ggctggggtt gattcccggt attccggcgg tgctggcgtt tttgttgagc
     5641 ggtcggcggg cttcgtttcc tcgcacctgg catgtgctct attttctgtt gctgttggcg
     5701 caggtggttt tactttgctg gcaaccgtgg ctgtggctga acggtgaatc cgttagcggt
     5761 atcggtctgg cgctggtagt ggcggatatt gtggcgttaa tctggctgct gaccaatcga
     5821 cgtttacgcg cttgttttaa tgaagaaaaa gaataacgca gaggtgcgct ttgtttatgc
     5881 cggatgcggc gtgaacgcct tatccggcct acaaaacatc gaaaattcaa tatattgcag
     5941 gagaaagtag gcctgataag cgtagcgcat caggcaattt tgtgtttgtc atcaaccttt
     6001 aacggcactt tttggtgaat ttgcactcca agcaacgtta ttgaataacc aaaggaagtg
     6061 acatgaaatc gctgcgttta atgttatgcg ctatgccgtt gatgctgacc ggctgttcca
     6121 cgatgtcgtc agttaactgg tcggctgcta acccgtggaa ctggtttgga tcgtccacca
     6181 aagtgagcga gcagggcgtg ggtgaattaa cggcgtccac accactgcaa gaacaagcca
     6241 ttgctgatgc gcttgatggc gattatcgcc tgcgcagcgg aatgaaaacc gcgaacggca
     6301 acgtggtgcg cttttttgaa gtgatgaaag gcgacaacgt ggcgatggtg attaacggcg
     6361 atcaaggcac gatcagtcgc attgacgtgc tggatagcga tattcctgct gacaccggtg
     6421 ttaaaatcgg tacaccgttt agcgaccttt acagcaaagc atttggcaat tgccaaaaag
     6481 ctgatggtga tgataatcgt gccgtcgaat gtaaagccga aggcagtcaa catattagct
     6541 accagttcag aggggaatgg cgtggtcctg aagggttaat gccttcggac gataccctga
     6601 aaaactggaa agtcagtaaa attatctggc ggcgttaatt tgcgtctgaa caaaccgccg
     6661 ccgcgaaaaa acacgtaaaa tagcgctcaa caatgccacg gattgcgtgg cattcttatt
     6721 ttcaggagga acaatgtctc aggttcagag tggcattttg ccagaacatt gccgcgcggc
     6781 gatttggatc gaagccaacg tgaaagggga agttgacgcc ctgcgtgcgg ccagtaaaac
     6841 atttgccgac aaactggcaa cttttgaagc gaaattcccg gacgcgcatc ttggtgcggt
     6901 ggttgccttt ggtaacaaca cctggcgcgc tctgagcggc ggcgttgggg cagaagagct
     6961 gaaagatttt ccgggctacg gtaaaggcct tgcgccgacg acccagttcg atgtgttgat
     7021 ccacattctt tctctgcgtc acgacgtaaa cttctctgtc gctcaggcgg cgatggaagc
     7081 ctttggtgac tgcattgaag tgaaagaaga gatccacggc ttccgttggg tggaagagcg
     7141 tgacctgagc ggctttgttg acggtacaga aaacccggcg ggtgaagaga cgcgccgcga
     7201 agtggcggtt atcaaagacg gcgtggatgc cggcggcagc tatgtgtttg tgcagcgttg
     7261 ggagcacaac ctgaagcagc tcaaccggat gagcgttcac gatcaggaga tgatgatcgg
     7321 gcgcaccaaa gaggccaacg aagagattga cggcgacgaa cgtccggaaa cctctcacct
     7381 cacccgcgtt gatctgaaag aagatggcaa agggctgaag attgttcgcc agagcctgcc
     7441 gtacggcacc gccagtggca ctcacggtct gtacttctgc gcctactgcg cgcgtctgca
     7501 taacattgag cagcaactgc tgagcatgtt tggcgatacc gatggtaagc gtgatgcgat
     7561 gttgcgcttc accaaaccgg taaccggcgg ctattacttc gcgccgtcgc tggacaagct
     7621 gatggcgctg taagcgtttc tgattctatg attaaaggcc agccagtaac aaaactgact
     7681 ggccttttta ttacttttgc ttcaacgccg catacacctg atcaactacc caaccataag
     7741 ttgcaatcgg taactgaccg ctttcgaaca tattgggatt tttttgtgga tcggcaactg
     7801 gcgaatgggg cttgttgctt aacatcacaa tcgccatatg attgaccgga tcgataacgg
     7861 ttactgtccc ggtccagcca gtgtgaccgt aggtttgcgg gcttgccagc gtgccaaacg
     7921 tcggcgtcat ggtggcatta ccattcacgc gccagccgag gccaaaagtg gcatcttcct
     7981 tagagctggt ggtgaacatc ttcaccgttt ccgcactgaa cagctgcaca tcgccatagc
     8041 ccccgccgtt cagcatcgtt tgcattaaca ccgcaatatc gccggtattg gaaaacaaac
     8101 ctgcgtgccc ggaaacgccg cccatcgaat agaaggcttt ttcatcgtgc acctgacccc
     8161 agagagtgga ggtgcggata ttcgggaaat ggatcacgcc atcgcgggta ttgccgttca
     8221 attccgtggc ggcaatttgt tgcggtttaa agcctttcag cagcgggtta aataccgtat
     8281 gtgtcaggcc gagcgggcga taaatcgact cttcaacata gcggtcaagc ggctgaccgg
     8341 taaccgactc gacgataaat ccaagcagca tataatcgac atcgctgtag atatgtttgc
     8401 tgccgggctg atattccagc ggcgtgcgct tgatcatctc cagcgtctgg cctttatcct
     8461 aggaatataa cgcgcccgcg accgctttat tcgggtattg cggatctgcc gggaaaccgc
     8521 cgctgtgatg cagcaggtca gaaatccgca gggtgttttt gcctttaatg gtgtcatttg
     8581 gactatcggc aaaccccgga atatatttcg caatccgatc gtcaggatgc agtttgcctt
     8641 cgctcatcag cttttgcagg gcgaagttcg tggcgtacat tttggtgttt gaggccaggt
     8701 catacagcgt cccggtggtg gctttgacag gctgttccat caacacgctg ccatcgtact
     8761 ttttcgccgc tccccaggct ttgcgataca caatctgatt atctttaatg atcagcaggt
     8821 ttacgccggg ataaccggca tcaacttgct ggctaatcca gcgatccatc tggttaagcc
     8881 gtttgacgtt aaaccctgct ttctctggcg agctttctgt cagaacaggg tattttgcgg
     8941 cgctgacgct acaactaaca gccagcagag aaagatagag cattgtccgt ttcatgatat
     9001 cagtccagat tgacgttacg gcagccaaag agcgtggtga aaataaaccc gcaaacccat
     9061 gccaccagaa tcccgccagc ataaaccgcc atggccggaa ggatgccttg tgcggaagtc
     9121 atcagcggca gcgctaccag accagacggc ccaaaggcgc tgtttaagcc catcggcaga
     9181 ccccaccagg ctatcaggcc gataaacaaa ccgcccgccg caccgcctaa acaggcggtg
     9241 acaaacggct tcatgcgggg cagggtgaca ccgtaaatca gcggttcacc aacgcccagc
     9301 aggccgggaa taatcgcccc gcgtacctga ctgcgtagcg cactgtgcgg ttgcgaccgc
     9361 cagtagagtg ccagcgccgc gcccacctgg cccgcgcctg ccattgaaag gatgggaaat
     9421 aagctgttga atccctggct gtccattaac gcgaggtaaa caggaataaa gccctgatgc
     9481 acgccaaata ccacggcgat caggaacagc ccggctaaaa ccgcacaacc gaacggatta
     9541 ctgttcaggt gcataaacag ccatgacata ccttcgaata accagccacc cagcggcata
     9601 atgatcaggt acgcaagcgt agcggtgatc agcagggtga ttaacgaggt cagcagcatg
     9661 tcgagatcgt ccggcataaa gcggcgcacc atgccttcaa tgcgggcgca ggcccacgcg
     9721 gcaatcagca cgccgataat attgccgcgc ggatcgatgg gcagaccaaa gaaatcgtga
     9781 aaaccggcgt agtaaccggt ggttgcagcg gggttataac cgagcaaaaa gagtgcggca
     9841 ataattgcgc cattgacgcc cgtgccgcca aatgcctggg cggcgttata gcccaccaga
     9901 atcaccagga aggtgaacaa acctttgctg aacaccttca taaaattcag cgcgtcgggt
     9961 agcgtgccct gagcatctgc cggaacgtgc atcaccgtgg caattaacgt cgctatcccc
    10021 agcagcagac cggcggcaat aaaaccgggg atcagcggcg tgaagatggt ggcgaatttg
    10081 gcgagaaatt gttgcacgcc ggaggtttgt ttggctttta actgacgttt gttctgggcg
    10141 gcgatttctg cggcatcctg taccggtgcc tcgcccagca gctcgctcat cgcttttgcg
    10201 gcacgatgcg ctttaccagg tccaaaaacg acctgcacct ggtcgctggt caaaatgacg
    10261 ctcttcacgc cttccagcgt tttgatattg ggatcaacca gtgaactgtc atgtacaccc
    10321 agacgcaggc gcgtcataca gttaccacaa ctggcgatat ttcccggtcc gccgacacgg
    10381 gtaagaatgg tgttcagaag ttcactgctg atctctttgg ccatgggtta ttccttgtct
    10441 aaaacctgac gaataaagcc gccgtgttga tccaggcgtt ttttagcttc tgcggcatcg
    10501 agatttttca gcaccatcac aatggccgtt ttacagttgc gctcgcaggc aattaacgcc
    10561 gcttccgctt gttcggcatt acatccggtg gcgtttttaa cgatattgac ctggcgcaca
    10621 tgcagttttt cgttggtggc gaccacatcg accataaggt tgccgaacac tttgccggat
    10681 ttaatcatca gcccggtgga aagcatattg agcaccagtt tctgcgctgt acctgctttc
    10741 atccgcgaag aaccggtaac aacttcggca cctacaatcg gtgtaatggc aaactcagcg
    10801 gtggttgaaa cggcgctccc cggattacag gaaattccca ctgtgcggca gccgagctgg
    10861 cgtgcgtatt ccagtccggc aatcacatac ggcgtgcgac cgctggcagc aatgccaacc
    10921 accacatcct gtgccgttaa attaatattt ttcagatcat taacaccgcc ttcccggcta
    10981 tcttccgcgc cttccaccgc gtgctgaatg gcatattcgc cgccagcaat caaaccaacc
    11041 accagacccg gtttcacgcc gtaggtgggc ggacattcgc tggcatccag aatccccaga
    11101 cgaccggatg ttcccgcacc gaggtaaatc agacgcccgc cgccgcttac ctgggcgtgg
    11161 ataacatcga tcgccgcggc gatatccggc agtacgcgct caacggcaag cggtacggtt
    11221 ttatcttcat cgttgataat ccggcacatt tccagcgttg atacgcggtc aatttcagcc
    11281 gaggcggcgt tcgagccttc agtaatcatc ttttcaagtt gcatcggtgg tgaccttact
    11341 atttacgatc aaataattgc agatgtatgg aatcatatat cctgaattga cagacttaaa
    11401 ggaatcattg agtctacact cgcagagtgt gaagagcgtc ataagaactt aagcggaatt
    11461 tatgctgcca gcccaggaga gtggaagatg ttgtacctga cgaaaataag caatgcgggg
    11521 agtgaattca ccgaaaacga gcaaaaaatc gctgactttc tgcaggccaa cgtgagtgag
    11581 ctgcaatccg tttcttcacg gcagatggcg aaacagctcg gcattagcca gtcgagcata
    11641 gtgaaatttg cccaaaaact cggcgcgcaa ggatttactg aattgcgtat ggcgttaata
    11701 ggcgaataca gtgccagccg ggagaaaacg aataccacgg cgctgcacct gcacagttcg
    11761 attaccagtg atgattcgct ggaagtgatt gcccgcaaat tgaatcgcga aaaagagctg
    11821 gcgctggagc aaacctgtgc gttgttcgac tacgcgcggc tgcaaaaaat cattgaggtc
    11881 atcagtaaag caccgtttat ccagataacg ggcctgggcg gatcggcgct ggtggggcgc
    11941 gatctgtcgt tcaaactgat gaaaattggc tatcgcgtgg cctgcgaagc cgacacgcac
    12001 gtacaagcca ccgtttccca ggcgttgaaa aaaggcgatg tacaaatcgc tatctcttac
    12061 agcggtagta aaaaagagat tgtcttgtgc gtagaagcgg cacgaaagca gggggcaacg
    12121 gtgattgcca ttacttctct ggctgactct ccgctgcggc ggctggcgca tttcacgctc
    12181 gataccgttt ccggtgaaac cgaatggcgt agctcgtcga tgtctacccg cacggcgcaa
    12241 aattctgtta ccgatttact gtttgttggt cttgtgcaac tcaatgatgt ggcgtcattg
    12301 aaaatgattc agcgcagcag tgagctaaca caacgcctga aatgatgcat aaagcagcga
    12361 ctggattgag attttcctga attagtgagc tgatccgcag caatattttg tttatcctgt
    12421 attttcagag ggaatggagt gtaacgctct gtattaacaa ggagagcatt aaaatgggta
    12481 aactcacggg caagacagca ctgattacgg gcgcattgca gggaattggc gaaggaattg
    12541 ccagaacttt tgcacgtcat ggcgcgaacc taatcttgct ggatatctcc cctgagatcg
    12601 aaaagctggc ggacgaactg tgtggtcgtg gtcatcgctg tacggcggtt gtcgccgatg
    12661 tgcgtgaccc ggcgtcggta gccgcagcta tcaaacgcgc gaaggaaaaa gaagggcgca
    12721 ttgatatcct ggtgaataac gcaggcgttt gtcgtctggg cagtttcctc gatatgagcg
    12781 atgacgatcg cgatttccat attgatatca atattaaagg cgtatggaac gtcacgaagg
    12841 cggtgctgcc ggagatgatt gcgcgcaaag atggtcgcat tgtgatgatg tcttcagtca
    12901 ctggtgatat ggtggccgat cctggcgaaa cggcgtacgc cttaacgaaa gcggcgattg
    12961 ttggcctgac aaaatcgctg gcggtggagt acgcgcagtc tggtattcgc gttaacgcca
    13021 tttgcccggg atacgtgcgc acaccaatgg cggaaagcat tgcccgccag tcgaacccgg
    13081 aagatccaga gtcggtgctg actgaaatgg cgaaagcaat cccgatgcgt cgcctcgccg
    13141 atccgctgga agtcggcgaa ctggcggcct tcctcgcatc ggatgaatcc agctatttaa
    13201 ccggtacaca gaatgtgatt gatggcggca gcacactgcc ggagacggtt agcgtcggta
    13261 tctgattcac ctctgtttcc tccctgcatg tgtggggagg atttcgtctt gaactaagtt
    13321 caccaggcta ttttatttgt cattttggcc ccgggcagtg ctcgaaatac tcacgtacta
    13381 tgtgtacgct ccggtttctc cgcgctgtcc gtgtccaaac tgcctgcaac aattacgcct
    13441 gttgaaccaa gttcttattc cctttttaac ttccaaatca ccaaacggta tataaaaccg
    13501 ttactccttt cacgtccgtt ataaatatga tggctattag aaagtcatta aatttataag
    13561 ggtgcgcaat ggccgttaac ttactgaaaa agaactcact cgcgctggtc gcttctctgc
    13621 tgctggcggg ccatgtacag gcaacggaac tgctgaacag ttcttatgac gtctcccgcg
    13681 agctgtttgc cgccctgaat ccgccgtttg aacagcaatg ggcaaaagat aacggcggcg
    13741 acaaactgac gataaaacaa tctcatgccg ggtcatcaaa acaggcgctg gcaattttgc
    13801 agggcttaaa aaccgacgtt gtcacttata accaggtgac cgacgtacaa attctgcacg
    13861 acaaaggcaa gctgatcccg gctgactggc agtcgcgcct gccgaataat agctcgccgt
    13921 tctactccac catgggcttc ctggtgcgta agggcaaccc gaagaatatc cacgactgga
    13981 acgacctggt gcgctccgac gtgaagctga ttttcccgaa cccgaaaacg tcgggtaacg
    14041 cgcgttatac ctatctggcg gcatggggcg cagcggataa agctgacggt ggcgacaaag
    14101 ccaaaaccga acagtttatg acccagttcc tgaaaaacgt tgaagtgttc gatactggcg
    14161 gtcgtggcgc gaccaccact tttgccgagc gcggcctggg cgatgtactg atcagcttcg
    14221 agtcggaagt gaacaacatc cgtaaacagt atgaagcgca aggctttgaa gtggtgattc
    14281 cgaaaaccaa cattctggcg gaattcccgg tggcgtgggt cgataaaaac gtgcaggcca
    14341 acggtacgga aaaagcagca aaagcctacc tgaactggct ctacagcccg caggcgcaga
    14401 ccatcatcac cgactattac taccgcgtaa ataacccgga agtcatggac aaactgaaag
    14461 ataaattccc gcagaccgag ctgttccgcg tggaagacaa atttggctcc tggccggaag
    14521 tgatgaaaac ccacttcacc agcggcggcg agttagacaa gctgttagcg gcggggcgta
    14581 actgatgttt gcagtttcat caagacgcgt gctgccgggc tttaccttaa gcctcggcac
    14641 cagtctgctg tttgtgtgcc tgattttgct gctgccgctc tccgcgctgg tgatgcagct
    14701 ggctcagatg agctgggcgc agtactggga ggtgatcacc aacccacaag tggtcgcggc
    14761 ctacaaagtg acgctgctgt cggcgtttgt ggcatcgatt tttaacggcg ttttcggtct
    14821 gctgatggcg tggattttaa cacgttatcg ctttccgggc cgcacgctgc ttgatgcgct
    14881 gatggattta ccctttgcgc tgccaacggc agtcgccggt ttaacgctgg cctcgctctt
    14941 ttccgtaaac ggtttttacg gtgaatggct ggcgaagttt gatatcaaag tcacctatac
    15001 ctggcttggt attgcggtgg cgatggcctt taccagcatt ccgtttgtgg tgcgtaccgt
    15061 gcagccggtg ctggaagagt taggcccaga atatgaagaa gcggcggaaa cgcttggtgc
    15121 aacgcgctgg cagagtttct gcaaagtggt gctgccggaa ctttctccag cgctggtggc
    15181 gggcgtggcg ttgtcgttta cccgtagtct tggtgagttt ggcgcggtga tatttatcgc
    15241 cgggaacatc gcttggaaga cggaagtgac gtcgctgatg atttttgtgc gcttgcagga
    15301 gtttgattac ccggcggcga gcgcgattgc ttcggtgatc ctcgcggcat ccctgctgct
    15361 gctgttctca attaacactc tgcaaagtcg ctttggtcgg cgtgtggtag gtcattaatg
    15421 gcggaagtta cccaattgaa gcgttatgac gcgcgcccga ttaactgggg caaatggttt
    15481 ctgattggca tcgggatgct ggtatcggcg ttcatcctgc tggtgccgat gatttatatc
    15541 ttcgtacagg cgttcagcaa agggctgatg ccggttttac agaatctggc cgatccggac
    15601 atgctgcacg ccatttggct gacggtgatg attgcgctga ttgccgtacc ggtgaacctg
    15661 gtgttcggca ttctgctggc gtggctggta acgcgcttta acttccccgg acgccagtta
    15721 ctgctgacgc tactggacat tccgtttgcc gtatcgccgg tggttgcagg tctggtgtat
    15781 ctgctgttct acggctctaa cggtccgctg ggcggctggc tggatgaaca taatttgcag
    15841 attatgttct cctggccggg aatggtgctg gtcaccatct ttgtgacctg tccttttgtg
    15901 gtgcgcgagc tggtgccggt gatgttaagc cagggcagcc aggaagacga agcggcgatt
    15961 ttgcttggcg cgtctggctg gcagatgttc cgtcgcgtca cattgccgaa cattcgctgg
    16021 gcgctgctgt atggtgtggt gctaaccaac gcccgcgcga ttggcgagtt tggtgcggtg
    16081 tcggtggttt ccggctcgat tcgcggcgaa acactttcgc tgccgttaca gattgaattg
    16141 ctggagcagg actacaacac cgtcggctcc tttaccgccg ccgcgctgtt gacgctgatg
    16201 gcgattatca ccctgttttt aaagagtatg ttgcagtggc gtctggagaa tcaggaaaaa
    16261 cgcgcgcagc aggaggagca tcatgagcat tgagattgcc aatattaaga agtcgtttgg
    16321 tcgcacccag gtgctgaacg atatctcact ggatattcct tctggtcaga tggtcgcgct
    16381 gctggggccg tccggttccg gaaaaaccac gctgctgcgc attattgccg ggctggagca
    16441 tcaaaccagc gggcatattc gcttccacgg taccgacgtg agccgcctgc acgcacgcga
    16501 tcgtaaagtc ggtttcgtgt tccagcatta cgcgctgttc cgtcatatga cggtgttcga
    16561 caatatcgct tttggcctga cggtgctgcc acgtcgcgag cgcccgaatg ccgcagccat
    16621 caaagcgaaa gtgacaaaat tgctggagat ggtccagctt gcgcatctgg cggatcgtta
    16681 tccggcacag ctttccggcg gccagaaaca gcgcgtggcg ctggcgcgcg cgctggctgt
    16741 tgaaccgcaa attctcctgc ttgatgaacc gtttggcgcg ctggatgcgc aggtgcgtaa
    16801 agagctgcgt cgctggctgc gtcaactgca tgaagagctg aaattcacca gcgtgttcgt
    16861 gacccacgac caggaagaag cgaccgaagt agctgatcgt gtagtggtga tgagccaggg
    16921 caatatcgag caggctgatg cgccggacca ggtatggcgc gaaccagcga cccgatttgt
    16981 gctcgaattt atgggcgaag tgaaccgcct gcagggaacc attcgcggcg ggcagttcca
    17041 tgttggcgca catcgctggc cgctggggta tacgcctgcg tatcaggggc cggtggatct
    17101 cttcctgcgc ccgtgggaag tggatatcag ccgccgtacc agtctcgatt cgccgctgcc
    17161 ggtacaggta ctggaagcca gcccgaaagg tcactacacc caattagtgg tgcagccgct
    17221 ggggtggtac aacgaaccgc tgacggtcgt gatgcatggc gacgatgccc cgcagcgtgg
    17281 cgagcgttta ttcgttggtc tgcaacatgc gcggctgtat aacggcgacg agcgtatcga
    17341 aacccgagat gaggaacttg ctctcgcaca aagcgcctga taggttgagt gaatgttaaa
    17401 cgcccggagg cgcttcccgc gatccgggct ttttaatggc aaggtttgta acctgtagac
    17461 ctgataagac gcgcaagcgt cgcatcaggc aacaccacgt atggatagag atcgtgagta
    17521 cattagaaca aacaataggc aatacgcctc tggtaaagtt gcagcgaatg gggccgaata
    17581 acggcagtga agtctggttg aaactggaag gcaataaccc ggcaggttcg gtgaaagatc
    17641 gtgcggcact ttcgatgatc gtcgaggcgg aaaagcgcgg ggaaattaaa ccgggtgatg
    17701 tgttaatcga agccaccagt ggtaacaccg gcattgcgct ggcaatgatt gccgcgctta
    17761 aaggctatcg catgaaattg ctgatgcccg acaacatgag ccaggaacgc cgtgcggcga
    17821 tgcgtgctta tggtgcggaa ctgattcttg tcaccaaaga gcagggcatg gaaggtgcgc
    17881 gcgatctggc gctggagatg gcgaatcgtg gcgaaggaaa gctgctcgat cagttcaata
    17941 atcccgataa cccttacgcc cattacacca ctaccgggcc ggaaatctgg cagcaaaccg
    18001 gcgggcgcat tacccatttt gtctccagca tgggaacaac cggcactatc accggcgttt
    18061 cacgctttat gcgcgaacaa tccaaaccgg tgaccattgt cggcctgcag ccggaagaag
    18121 gcagcagtat tcccggcatt cgccgctggc ctgcggaata tctgccgggg attttcaacg
    18181 cttctctggt ggatgaggtg ctggatattc atcagcgcga tgcggaaaac accatgcgcg
    18241 aactggcggt gcgggaagga atattctgtg gcgtcagctc aggcggcgcg gttgctggag
    18301 cactgcgggt ggcaaaagct aaccctggtg cggtggtggt agcgatcatc tgcgatcgtg
    18361 gcgatcgcta cctctccacc ggagtgtttg gggaagagca ttttagccag ggggcgggga
    18421 tttaagatta ttgcgcaggc accgttttat ccgtctgcgc aattagcgca tgtaaaaaag
    18481 gctgtgcgtt ctcatcgctc ttaccgggga tcttcacaat atacggcttc ccggtaatcg
    18541 atgacgacga agcaacctta tcaataaact gctcggcagt gtcaatgcgg ttacgggtat
    18601 tgccgagctt cagacgcaga tgagaaaccg cttcatcgca ggtatgttca tcaccgttgc
    18661 gcacaaatat caaatccttt ttctgtgata atccctccag catggcgttg atgcgggctt
    18721 cttcatgggc agttaacttc gcgtaagcgg ggagtgtcat tagtagtgta atgaccagac
    18781 aaatgatttt cttcattgaa ttaccttatc cttgttttgt aatagccgat ttgactgcca
    18841 gaatcaggta aagtttcggt tggaatacga tgaggatcat cggcaaggag aaaaaattaa
    18901 atgagtagtt tgttgttgtt taacgataag agcagggcgc tgcaggcgga tatcgtcgcc
    18961 gtgcagtcgc aggtggttta cggcagcgtg ggcaacagca ttgccgtgcc tgctatcaaa
    19021 cagaacggtc tgaatgtttt tgccgtgccg acggtattgc tgagcaatac gccgcattat
    19081 gacactttct acggtggtgc gattccggat gaatggttta gcggttattt gcgtgcgctt
    19141 caggagcgtg atgcgctgcg ccagctgcgc gctgtaacca cgggctatat gggaacggca
    19201 tcgcaaatca aaatccttgc cgagtggctg actgcgctac gcaaagacca tcctgaccta
    19261 ttgatcatgg tcgatccggt gattggcgat attgatagcg gaatttatgt caaacctgac
    19321 cttcccgagg cgtatcgaca atatttactg ccgctggcgc agggaattac ccccaatatc
    19381 tttgagctgg aaatcctgac cggtaaaaat tgccgcgatc tcgacagtgc cattgctgcc
    19441 gcaaaaagtc tgctttcaga cacattaaaa tgggtggtga ttaccagcgc ctccggtaat
    19501 gaagaaaatc aggagatgca ggtggtggtg gtcagtgccg acagcgtgaa tgtcatttcc
    19561 cattcacggg taaaaaccga cctgaaaggg actggcgacc tgttttgtgc tcagcttatc
    19621 agtggtttgc tgaaagggaa ggcgttaaac gatgcggtgc accgtgcggg gttgcgcgta
    19681 ctggaagtga tgcgctacac ccagcagcat gagagcgatg aattgatttt gccgccgctg
    19741 gcggaagcat aaaaaatggc gccgatgg
//
LOCUS       DAJTNN010000068        19489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTNN010000068 DAJTNN010000000
VERSION     DAJTNN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.068"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1231
                     /locus_tag="OO150_002098"
     CDS             <1..1231
                     /locus_tag="OO150_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5516246.1"
                     /translation="FFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSS
                     LESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLML
                     LAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIIC
                     QAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPL
                     GIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALA
                     LAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQA
                     VNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTWH
                     DFLPSGTASVSEKHSER"
     gene            1221..2936
                     /gene="hyfG"
                     /locus_tag="OO150_002099"
     CDS             1221..2936
                     /gene="hyfG"
                     /locus_tag="OO150_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR5516247.1"
                     /translation="MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            2946..3485
                     /gene="hyfH"
                     /locus_tag="OO150_002100"
     CDS             2946..3485
                     /gene="hyfH"
                     /locus_tag="OO150_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5516248.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            3482..4240
                     /gene="hyfI"
                     /locus_tag="OO150_002101"
     CDS             3482..4240
                     /gene="hyfI"
                     /locus_tag="OO150_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5516249.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            4233..4646
                     /gene="hyfJ"
                     /locus_tag="OO150_002102"
     CDS             4233..4646
                     /gene="hyfJ"
                     /locus_tag="OO150_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5516250.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHRSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            4676..6688
                     /gene="hyfR"
                     /locus_tag="OO150_002103"
                     /pseudo
     CDS             4676..6688
                     /gene="hyfR"
                     /locus_tag="OO150_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            6710..7555
                     /locus_tag="OO150_002104"
                     /pseudo
     CDS             6710..7555
                     /locus_tag="OO150_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(7593..8654)
                     /locus_tag="OO150_002105"
     CDS             complement(7593..8654)
                     /locus_tag="OO150_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5516251.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            8867..10330
                     /gene="bepA"
                     /locus_tag="OO150_002106"
     CDS             8867..10330
                     /gene="bepA"
                     /locus_tag="OO150_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5516252.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSTPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            10351..10710
                     /gene="arsC"
                     /locus_tag="OO150_002107"
     CDS             10351..10710
                     /gene="arsC"
                     /locus_tag="OO150_002107"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5516253.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(10848..11594)
                     /gene="hda"
                     /locus_tag="OO150_002108"
     CDS             complement(10848..11594)
                     /gene="hda"
                     /locus_tag="OO150_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5516254.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11644..12933)
                     /gene="uraA"
                     /locus_tag="OO150_002109"
     CDS             complement(11644..12933)
                     /gene="uraA"
                     /locus_tag="OO150_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5516255.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(13019..13645)
                     /gene="upp"
                     /locus_tag="OO150_002110"
     CDS             complement(13019..13645)
                     /gene="upp"
                     /locus_tag="OO150_002110"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5516256.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            13969..15006
                     /gene="purM"
                     /locus_tag="OO150_002111"
     CDS             13969..15006
                     /gene="purM"
                     /locus_tag="OO150_002111"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5516257.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            15006..15644
                     /gene="purN"
                     /locus_tag="OO150_002112"
     CDS             15006..15644
                     /gene="purN"
                     /locus_tag="OO150_002112"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5516258.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKIHENAAWLDGQRLPPQGYAADE"
     gene            15816..17882
                     /gene="ppk1"
                     /locus_tag="OO150_002113"
     CDS             15816..17882
                     /gene="ppk1"
                     /locus_tag="OO150_002113"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5516259.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNPEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            17887..19428
                     /gene="ppx"
                     /locus_tag="OO150_002114"
     CDS             17887..19428
                     /gene="ppx"
                     /locus_tag="OO150_002114"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5516260.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
BASE COUNT         4702 a         5094 c         5093 g         4600 t
ORIGIN      
        1 tttcttccat ctgtttttgt tcaccatgct gctggttgtt accagcaata acctgattgt
       61 gatgtgggcg gcgatcgaag ccaccacctt aagctcggcg tttctggtag gcatttacgg
      121 tcagcgttca tcgctggaat cagcatggaa gtacatcatt atttgtactg ttgatgtcgc
      181 ttttggtctg ttcggtaccg tgctggtata cgccaacgcc gccagcgtta tgccgcaggc
      241 agaaatggcg atattctgga gcgaggttct taagcaatcg tccttgcttg acccaacatt
      301 aatgctgttg gcctttgtgt ttgtgctaat tggctttggt accaaaaccg ggctatttcc
      361 catgcacgcc tggctgccgg atgctcacag tgaagcgccg agtccggtca gcgccctgct
      421 ctccgccgta ttgctgaact gcgcgctgtt ggtgctgatt cgctattaca tcattatttg
      481 ccaagccatc ggcagcgatt tccccaaccg gttgttgctc atcttcggca tgttgtcggt
      541 tgccgtggcg gcatttttca ttctggtaca gcgggacatt aagcgtctgc tggcgtactc
      601 cagcgtggag aacatggggc tggtcgcggt ggcgctaggc attggcgggc cgctgggaat
      661 ttttgccgcg ctgctgcaca ccttaaacca cagtctggca aaaacgctgc tgttctgcgg
      721 ttccggcaat gtactgctca agtacggcac gcgcgatctc aacgtcgtct gtgggatgct
      781 caaaatcatg ccatttaccg ccgtgctgtt tggcggcggt gcgctggcgc tggcagggat
      841 gccgcccttc aacatttttc ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa
      901 tcacctgctg attatcgtcc tgctgttatt gctgttaacg ctggtgctgg cgggcctagt
      961 acggatggct gcgcgggtgt taatggcgaa accgccgcag gccgttaacc ggggtgatct
     1021 cggctggttg accacctcgc caatggtgat tctgctggtc atgatgctgg cgatgggaac
     1081 gcatattcca caacctgtca tcaggatcct ggcgggcgct tccactatag tcctctcagg
     1141 gacgcacgac ctgcctgcac aacgtagcac ctggcatgat tttttgcctt caggcaccgc
     1201 atctgtttcg gagaaacaca gtgaacgtta attcatcgtc aaatagtggc gaagcgattc
     1261 tcgccgccct gaaaacgcag ttccccggcg cggtactgga tgaagagcga caaacgcctg
     1321 aacaggtcac cattacggtg aaaatcaatc tgctgcctga cgttgtacat tatctttatt
     1381 atcaacatga tggctggctt ccagtcctgt ttggcaacga cgagcggaca cttaacggtc
     1441 attacgcggt ttattatgcc ctttcaatgg aaggggccga aaaatgctgg atcgtggtga
     1501 aggcactggt cgatgccgac agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg
     1561 cggtctgggg cgagcgagaa attcgcgata tgtacgggct gattccggtt ggcctgccgg
     1621 atcagcgtcg cctggtgttg cccgatgact ggccggaaga tatgcatccg ctgcacaaag
     1681 atgcgatgga ttatcgactg cgccctgaac cgacgacaga ttccgaaacg tatgcgttta
     1741 tcaatgaggg caacagcgat gcgcgggtga tccctgtcgg cccgctgcat atcacctccg
     1801 atgaaccggg tcacttccgc ttgtttgtgg atggcgagca aattgtcgat gctgattacc
     1861 gcctgtttta tgtccatcga ggcatggaga aactggcaga aacgcggatg ggctacaacg
     1921 aagtgacctt cttatcggac cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct
     1981 ataccaactc ggttgaaaat gcactgggga ttgaggtgcc gcaacgagcg cataccattc
     2041 gctcgattct gctggaagtc gaacggctac acagtcattt gctcaacctt ggcctctcct
     2101 gccattttgt tggttttgat accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga
     2161 tgacgatggc ggaattgctg accgggtcgc gtaaaaccta cggtctgaat ctgattggtg
     2221 gtgttcgccg cgatattctc aaagagcaac gtctgcaaac gctgaaactg gtgcgcgaga
     2281 tgcgcgccga cgtgtcggag ctggtagaga tgctgcttgc tacgccgaat atggaacaac
     2341 gcactcaggg cattggcatt ctcgaccgac aaatcgcccg tgattatagc cctgtagggc
     2401 cgctgatccg cggcagtggt tttgcccgtg atttgcgctt tgatcacccc tacgccgact
     2461 acggcaatat tccaaaaaca ctgtttacct tcaccggcgg cgatgttttc tcccgcgtga
     2521 tggtccgtgt caaagagacg tttgattcgc tggcaatgct ggaatttgcc ctcgacaaca
     2581 tgccggatac cccactgctg accgaaggct ttagctataa acctcacgca ttcgcgctgg
     2641 gctttgttga agcgccacgc ggtgaagacg tgcactggag catgctcggt gataaccaaa
     2701 aattgttccg ctggcgctgc cgtgccgcca cctacgccaa ctggccggtg ttgcgttaca
     2761 tgctgcgcgg caataccgtt tctgacgcac cgctgattat cggtagcctt gatccctgct
     2821 actcctgtac cgaccgtgtg acgctggtag atgtgcgcaa gcgccagtca aaaaccgtgc
     2881 cgtataaaga gatcgaacgc tacggcattg atcgtaaccg ttcgccgctg aagtaaggac
     2941 agaagatgct gaagttactg aaaactatta tgcgcgccgg aaccgcgacg gtgaaatatc
     3001 ccttcgcgcc actggaggtc agcccaggct ttcgcgggaa accggacctg atgcccagcc
     3061 aatgtattgc ctgcggcgcc tgtgcttgtc cggcaaatgc gctgactatc cagaccgacg
     3121 accagcaaaa ttcgcgcacc tggcagctct atctggggcg ttgtatttac tgcggacgtt
     3181 gtgaagaagt gtgcccgacc agagccatcc agcttaccaa taactttgaa ctgaccgtca
     3241 ccaataaagc cgatctctat acccgcgcga cgttccatct acaacgttgc agccgttgcg
     3301 aacgcccgtt tgccccgcaa aaaaccgtcg cactggctgc tgaattgttg gcacagcaac
     3361 aaaatgcgcc acaaaaccgc gaaatgttgc gggcgcaagc gagcgtctgc ccggagtgca
     3421 aacaacgcgc gacgctgatc aacgacgata cagatgtacc gctggtggct aaggagcagc
     3481 tatgagtcca gtgcttacac aacatgtcag ccagcccatc acgctggatg agcaaacgca
     3541 aaagatgaag cggcatttgc tacaggatat ccgtcgctca gcctacgttt atcgcgtcga
     3601 ttgcggcggc tgcaacgcct gtgaaatcga aatttttgct gccatcaccc ccgtattcga
     3661 cgcagaacgt tttggcatta aggttgtttc atcaccgcgt cacgccgata ttttgttatt
     3721 tactggcgca gtcacccggg cgatgcgtat gcctgcactt cgggcgtatg agtctgcccc
     3781 cgatcataaa atctgtgttt cctacggcgc gtgcggtgtc ggcggcggta ttttccacga
     3841 tctctacagc gtctggggcg gcagcgacac cattgtcccc attgatgttt ggatccccgg
     3901 ctgcccgcca acaccggccg ccaccattca cggtttcgcc gtggcgctcg gtttgctgca
     3961 acagaagatt cacgctgtgg attatcgcga tcccaccggg gtgactatgc aaccgttgtg
     4021 gccacagatc ccgccatcac agcgtatcgc cattgagcga gaagcgcggc ggctggcggg
     4081 ctatcgtcag gggcgagaaa tttgcgatcg gctcctgcgc catttaagcg acgatcctac
     4141 cggaaatcgg gttaacacct ggttgcgcga tgccgacgat ccacgtctca atagtatcgt
     4201 tcagcaactc tttcgcgtac tccgggggtt acatgactga agagtgcggg gaaattgttt
     4261 tctggacgct gcgaaaaaag tttgtcgcca gtagcgacga gatgccggaa caccgctctc
     4321 aggtaatgta ttactcgctg gctatcggcc atcacgttgg cgtgattgat tgtctgaatg
     4381 tcgccttccg ctgcccactg acggaaaacg aagattggct tgcacaggtc gaagaggagc
     4441 aagcccgacg taagatgctg ggggtgatga cctttggtga gattgttatt gacgccagcc
     4501 acaccgccct gttgacccgg gcattcgcgc tactggcaga tgacgcgacg tctgtgtggc
     4561 aggcgcgtag cattcaattc attcatctgt tggatgaaat tgtgcaggaa ccggccatct
     4621 atctgatggc cagaaaaatt gcgtgagaag gatttctcat taataaggac tgttgatggc
     4681 tatgtcagac gaggcgatgt ttgccccgcc acaaggaata acaattgaag cggtaaacgg
     4741 aatgctcgcg gagcggttag cacagaaaca cggtaaggcg tctttattac gcgccttcat
     4801 cccgatgccg ccgccgttca gcccggtaca acttattgaa ctgcatgtta tcaaaagcaa
     4861 cttctattac cgctaccatg atgatggcag cgatgtgacg gcaacaacag agtatcaggg
     4921 cgagatggtc gattattcgc gtcacgccgt ccttctcggc agtagtggaa tggcggagct
     4981 acgctttatt cgtacccacg gcagccgttt tactccctag gattgcacac tgtttaactg
     5041 gctggcacgc attatcactc cggttctgca atcatggctc aatgatgaag aacaacaggt
     5101 ggcgctgcgt ttgctggaga aagatcgcga tcatcatcgg gtactggttg atattactaa
     5161 tgcagtgctg tcacatcttg atctcgacga tctgatcgct gacgtcgctc gtgagatcca
     5221 tcattttttc ggtctggctt cagtcagtat ggtactgggc gatcatcgaa agaacgagaa
     5281 gtttagcctg tggtgcagcg atctttctgc ctcacattgt gcgtgtctgc cacgcaatat
     5341 gcctggcgac agtgtattgc tgacacaaac gctacaaacc cgacaaccga ccttgacgca
     5401 ccgtgcagac gatctgtttc tctggcaacg cgacccgtta ttactcttac ttgcatctaa
     5461 cggctgcgaa tctgcgctcc ttataccgct tacctttggc aaccatacac cgggtgcatt
     5521 gttgctggcg catacctctt ccactctctt tagtgaggaa aactgccagc tactacaaca
     5581 catagccgat cgcatcgcta ttgccgttgg caatgccgat gcctggcgta gcatgaccga
     5641 tttgcaggaa agtttgcagc aagaaaacta ccagcttagc gagcagctcc tttcgaatct
     5701 gggcatcggt gacattatct atcaaagcca ggcaatggaa gacctgctcc agcaggtaga
     5761 tattgtagcg aagagcgaca gtacggtgtt gatttgtggt gaaaccggaa ccggcaaaga
     5821 ggtgatcgcc agagcgctcc atcaacttag cacgcgacgc gacaaaccgc tggtcaaaat
     5881 caactgcgct gccatccccg ccagtcttct ggaaagtgag ttattcggtc atgacaaagg
     5941 ggcgtttact ggtgcgatta atacacatcg tggtcgtttt gaaattgccg atggcggcac
     6001 gttgtttctc gatgaaattg gcgatctgcc gttagaactt cagcctaaac tgctgcgcgt
     6061 attgcaggaa cgggagattg agcgtctcgg cgggagtaga acgatcccgg tgaatgtcag
     6121 agtcattgcc gccaccaacc gtgatttgtg gcaaatggtt gaagatcgcc agtttcgcag
     6181 caatctcttt tatcgcctga atgtcttccc attggaattg ccgccgctac gcgaccgtcc
     6241 ggaagatatc cctcttttag caaaacattt cacgcaaaaa atggcgcgcc atatgaatcg
     6301 cgcaattgac gccatcccga ccgaggcgct acgccagttg atgtcgtggg actggccggg
     6361 caacgtgcac gagctggaaa acgtgattga gcgagcggta ctgctgactc gcggtaacag
     6421 tctgaattta catcttaatg tccgacaaag ccgtttactg ccgacgctaa atgaagattc
     6481 agcgcttcgc agttcaatgg cgcagttgct gcacccgacg acgccagaga atgacgaaga
     6541 agaacgtcag cgcattgttc aggtattgcg agaaaccaat ggcattgttg ccgggccccg
     6601 tggcgcggcg acacgattag ggatgaagcg caccacgctg ctgtcacgaa tgcagcgtct
     6661 ggggatctcg gttcgcgagg tgttgtaatc tgcttttgca ggagtatgca tgagaaacaa
     6721 actctctttc gacttgcagt tgagcgccag aaaagcggca atcgctgaac ggattgccgt
     6781 ccataaaatt gcccgcagta aagtgtcggt ctttttaatg gcgatgtccg ctggcgtgtt
     6841 tatggcgatc ggatttactt tttacctttc cgttatcgcc gatgccccgt cttcacaggc
     6901 attaacccat ctggtgggcg gcctttgctt tacactcggc tttattttgc tggcggtttg
     6961 cggcacagcc tgttcacctc gtcggtaatg acggtgatag caaaaagtcg gggcgttatt
     7021 agttggcgaa cttggctgat taacgcactt ctggtggcct gtggtaatct ggcaggtatt
     7081 gcctgtttca gtttgttaat ctggttttcc gggctggtga tgagtgaaaa cgcgatgtgg
     7141 ggagtcgcgg ttttacactg cgccgagggc aaaatgcatc atacatttac tgaatctgtc
     7201 agcctcggca ttatgtgcaa tctgatggtt tgcctggcgc tgtggatgag ttattgcggg
     7261 cgttcgttat gcgacaaaat cgtcgccatg attttgccca tcaccctgtt tgtcgccagt
     7321 ggctttgagc actgtatcgc caatttgttt gtgattccgt tcgccattgc cattcgccat
     7381 ttcgcccccc cccttctggc aactggcgca cagtagcgca gacaattttc cggcactgac
     7441 ggtcagccat tttattaccg ccaatctgct cccggtgatg ctgggtaata ttatcggcgg
     7501 tgcagtgctg gtgagtatgt gttatcgggc tatttattta cgtcaggaat cctgaaaaat
     7561 cagcccggcg aaacagttcg tcgggctgaa gattattctt gcgcgatttg cccatcgggc
     7621 caggcgtgga tcacggcttt gatcagcgtc gccaatggaa tggcgaagaa tacgccccag
     7681 aatccccaca aaccaccgaa gatcaccacc gataaaataa tcaccagcgg atgcaggtta
     7741 accgcttcgg agaacaacac cggtactagc aggttgccgt ccagcgcctg aataatcaga
     7801 tacaccgcga agcagctcca gaattccgtg cctgcgccaa actggaatag cgccacgcca
     7861 accaccggaa tggtcaccac aaatgcgcca atgtacggaa taagaaccga gaagccgacc
     7921 agcaccgcca gcagcagcga atagttcagc ccaaagagca agaaccccag ccaggtggcg
     7981 atccccacca cgatcatctc cagcactttg ccgcggatat agttggtgat ttgttgattc
     8041 atctccttcc acacctgtcc tgccagtcca cggttgcgcg gcagcacccg acgaacggcg
     8101 ttcagcatct gctctttgtc tttcagcagg aagaagacca tcaatggcac cagcaccaga
     8161 tagacggcaa tggtcagcaa accgaccagc gaggcgaggg aaattttcac caccgaatcg
     8221 cccatggtca gcatccgact gcgcatattt tcggccattg catcaataat gcccgcgtcc
     8281 attaacgccg gatagcggcg cggcaacgtg gcggcaaagt cagaaagctt attgagcatc
     8341 cccggcatat cgcggattaa gtagatgccc tgttgccagg cgataggcag taccacgaac
     8401 gccatcagta gcaatatacc gacaaaaacc accaatacaa tcgacgtcgc ccagcggcgg
     8461 gagcagccaa tagattgcag gcgcacggtt ggccattcca gcaaataggc cagcacaata
     8521 gccaccagca acggagcaag caggccacta aagaaaaaga taatgccaaa tccggcaact
     8581 aaaataacca gcaaggcaat cgcttccggg tcgctaaaac ggcggcgata ccattgcatc
     8641 aacatttcga gcataaaaac cttccctgaa cctcaagagc gggattgcga tccgcaattg
     8701 tatcgaaatg tcacaaaaaa gacttcgctt tttatgacgg attcaggaaa ctgaaaagtc
     8761 atttgagtgg gctaatcttc gccgttacac tcaaaggcgg cgcggtggga acgatatttc
     8821 acagtatcgg tcaaatgact atcttcagaa atacaggata gaggttatgt tcaggcagtt
     8881 gaaaaaaaac ctggttgcaa ccctcattgc tgctatgacc attggtcagg tagccccggc
     8941 gtttgccgac aacgcagaca ccttgccgga tatgggaacc tccgcaggaa gcacgctttc
     9001 cattggtcag gaaatgcaga tgggcgacta ttatgtccgc cagctacgcg gcagcgcgcc
     9061 gttaattaat gacccgctgt taacgcaata tattaattcg ctggggatgc gtctggtttc
     9121 gcatgccaat tcggttaaga caccgtttca tttttttcta atcaacaacg acgaaattaa
     9181 cgcctttgct ttctttggcg gcaacgtggt gctgcactct gccctgttcc gttattccga
     9241 taacgaaagt caactggctt cagttatggc gcacgaaatc tcccacctca cccaacgtca
     9301 cctggcgcga gcgatggaag atcagcagcg cagcacgccg ctgacctggg tcggcgcgtt
     9361 aggttctatt ttactggcga tggccagtcc gcaggcgggg atggcggcgc tgaccggtac
     9421 actggcggga acgcgtcagg ggatgatcag tttcacccag caaaatgaac aggaagcgga
     9481 ccgcattggt attcaggtgc tgcaacgctc gggattcgat ccgcaggcga tgccaacctt
     9541 cctcgaaaaa ttactcgatc aggcgcgtta ctcctcgcgc ccgccggaaa ttttattgac
     9601 tcacccgttg ccggaaagtc gtctggcaga tgcccgcaac cgtgctaatc agatgcgacc
     9661 gatggtggtg cagtcgtcgg aagatttcta tctggcgaaa gcgcgcacac tggggatgta
     9721 taattccgga cgtaaccagc tcaccagtga tttgctggat gaatgggcga aaggaaacgt
     9781 tcgtcagcaa cgagcggcgc aatatggtcg tgctttacag gcgatggaag ccaataaata
     9841 cgacgaggcg cgtaaaacgc tgcaaccgtt actggcggca gaacctggca acgcatggta
     9901 tctcgatctg gctaccgata tcgatcttgg gcaaaacaaa gccaatgagg caatcaatcg
     9961 cctgaaaaat gcccgtgatt tgcgcaccaa tccggtgttg cagctcaacc tggcaaacgc
    10021 ttatctgcaa ggcggtcaac cacaagaagc ggccaatatt ctcaatcgct acacctttaa
    10081 taataaagat gacagcaacg gctgggattt actggcacag gcggaagccg cgctaaataa
    10141 ccgcgatcag gagctggctg cgcgagcaga aggttatgcg ctcgccggac gactcgatca
    10201 ggccatttcg ctgttgagta gcgccagttc gcaggtgaaa ttaggcagcc tgcaacaagc
    10261 gcgttacgat gcgcgcatcg accagttgcg ccagctgcag gaacgcttta agccttatac
    10321 caagatgtaa ttcgcattaa aggaagaaca atgaccaaac aggtaaaaat ctaccataac
    10381 ccacgctgtt caaagagccg ggaaacgctg aacctgctga aagaaaacgg cgtggagcca
    10441 gaagtggtac tctaccttga gacacccgcc gatgcggcaa cgctgcgcga tttgctgaaa
    10501 atgctgggga tgaatagcgc ccgtgaattg atgcgccaga aagaggatct ttataaagaa
    10561 ctgaacctgg ctgacagttc gcttagcgaa gaggcgctga ttcaggcaat ggtggataat
    10621 ccgaagctga tggagcgccc gattgtggtc gcgaatggca aagcgcggat tggtcgtcca
    10681 ccggagcagg tactggaaat cgtgggttaa gatgtgctcc atcgctagtt gaagcacatt
    10741 gccggatgcg acgcttgcca tgtcttatcc ggcctacgaa tactatggat taacttcgcg
    10801 tagttcggat aaggcgttcg cgccgcatcc gacaataaac accttatcta caacttcaga
    10861 atttctttca caaacggaat ggtcagctta cgttgcgcgg taatcgacgc acgatccaac
    10921 tgatccaacg tcataaatag cgtgcgcatt tctctgtcga gccgcttcag caagaaacgc
    10981 cccacatctt ccggcagttc aaaaccacgc aaacgcgcgc gtaactgtag cgcctgcaac
    11041 ttatcttcat cagaaagtgg ctgcaatttg tagatctgcc cccagtcgag tcgcgacgcg
    11101 agatccggta atcccagatt caactgccgc ggtggacgat cgccggtgat caacaaccgt
    11161 gttttgcccg attccagaat tcgattgtag agatcgaaaa tcgccatctc ccacaactca
    11221 tcgcctgcaa tacactcaat gttgtcgata caaaccagcg acaaatgctc cataccgtcg
    11281 agcacttccg gaacaaacca agtgcgttta tccagcggga catagcccac cgcatcgcca
    11341 cgctgcgaca attccgcgca agccgcgtgc agcagatggc tgcgccccgc gccttcgcgt
    11401 gcccagagat agatgtaacc gctatgttcc tgacgcagca cgttttgcag cgcggccagt
    11461 aaagaggagt tatcccccgg ccagaaactt gcaaaggttt cgtcgtcagg aagataaagt
    11521 ggcaaagaga gctgtgccgg tgtgttcaga gatacctcaa ccaggatttc acaaaatcgc
    11581 gagaagttta ccacagaatc ccatgatgtt tgaaccgggc agcaacactg cccggtcggt
    11641 acattatttg tctgttatat ccgcctcttc tgcgtccagc accacttctt cagggcgcag
    11701 cacactgatc agtttgaaaa tcaaacttaa gccgataccg acgatggtcg ccagcgccat
    11761 accttttaac tccgccgcgc cgatgtttac cttcgcgcca ctgacgccga tgatcaaaat
    11821 cacggaagtc aggatcaggt tctgtgcttt gttgtaatcc actttcgatt cgatcaaaac
    11881 acgaataccg gaagcaccga tgacaccata aagcagcagc gaaacgccgc ccatcaccgg
    11941 caatgggatc atctggatag cggcagccag tttaccgacg caggaaagca ggatagcgaa
    12001 aatcgccgcc ccgccgataa cccaggtact gtaaacacgg gtgatcgcca tcacgccaat
    12061 gttttctccg taagtggtat ttggcgtgga gccaaagaag ccggagatca cggtcgacag
    12121 gccgttagca aacatcgaac ggtgcagacc tggatcgcgc agcagatctt ttttgacgat
    12181 attagccgtt actaccaggt gccctacgtg ttcggcaata accactaacg ccgctggcag
    12241 aatagtcaga atggcaaacc actcgaagcg cggcgtatag agggttggca gcgcaaacca
    12301 gtgagcatta ataatcggcg tggtatcgac aattcccatt gcgaaagaga gcgcgtagcc
    12361 caccagcacg ccaattaaaa tcgggataat tgccaggaaa ccacgaaaca gcacggaacc
    12421 taaaaccgtg accgccaggg tggtaataga gatgatgatg gttttggagt ctggcgtttg
    12481 cccttcagcc gggagtaaac ccgccatacc ggcagctacg cccgccagct ccagaccgat
    12541 gacggcaacg attgcgccca ttgccgcagg tggaaacagc acgtccagcc agccggtccc
    12601 cgctttcttc acgataaaag aaaccaggca gaacagcacg ccgcacataa taaagccgcc
    12661 cagcgcgact tcatacccta acggcaacag taacaatacc ggtgaaataa aggcaaagct
    12721 ggaaccaaga taagccggaa ttttcccttt acagatgaag agatacagca gcgttccaat
    12781 accgttaaat aacagtacag tcgccgggtt aatatgaaat aagacgggca ccaggacggt
    12841 tgcaccaaac atggcgaaca aatgttgcaa actaagcggg attgtctgta aaagtggcgg
    12901 tctttcactc accccgatag cacggcgcgt catagtatta tcctctgtat tatgtgttat
    12961 aggcgcttta ctcaaaaaaa agccgactct taaagtcggc tttaattatt tttattcttt
    13021 atttcgtacc aaagattttg tcaccggcat cgccgaggcc cggaataatg tatccgtgct
    13081 cgttcagtcc ctgatcaatc gatgcggtat acagttcgac gtccgggtgc gctttttcca
    13141 gcgcagcgat accttctggc gcagcgacca gcaccagaac tttgatgctg ctgcagcccg
    13201 cttttttcag caggtcgatg gtcgcgataa cggaaccacc ggttgccagc attgggtcaa
    13261 cgatcagcgc catacgctca tcgatgttag aaaccagttt ctggaagtac ggtaccggct
    13321 ccagcgtttc ttcattacgg tacataccga caacgctgat gcgcgcgctc ggaacatttt
    13381 ccagcacacc gtccatcata ccaagacccg cacgcagaat tggcacaacg gtaattttct
    13441 tacctttgat ctggtcgatt tctaccgggc cgttccagcc ttcgatagtt actttttccg
    13501 tttcgaggtc ggcggtcgct tcgtaagtca gcaggctacc cacttcggaa gcgagttcgc
    13561 gaaagcgctt ggtgctgata tcttgctcac gcatcagtcc cagcttgtgt ttgacgagtg
    13621 ggtgtttgac ttccacgatc ttcatactct ttctcctttg aggggcagcc acaaaaaaat
    13681 cgacggatta tacctccttt cttcaaggcg gcaatattct tttcgttgac tttagtcaaa
    13741 atgataacga tttgagataa agttatttta tattcaaatg gttatgaaag aagatcattc
    13801 catccgaaaa ctaacctttg ccctggcaca agtcttcttt cgccgcgcgt ctggggaaaa
    13861 gacgtgcaaa aaggttgtgc aaagcagcct cgcaaacgtt tgctttccct gttagaattg
    13921 cgccgaattt tatttttcta ccgcaagtaa cgcgtgggga cccaagcagt gaccgataaa
    13981 acctctctta gctacaaaga tgccggtgtt gatattgacg cgggtaatgc tctggttgga
    14041 agaatcaaag gcgtagtgaa gaaaacgcgt cgtccggaag tgatgggcgg tctgggcggc
    14101 ttcggtgcgc tgtgtgcatt gccgcaaaaa tatcgtgaac ccgtgctggt ttccggcact
    14161 gacggcgtag gtaccaagct gcgtctggca atggacttaa aacgtcacga caccattggt
    14221 attgatctgg tcgccatgtg cgttaatgac ctggtggtgc aaggtgcaga accgctgttt
    14281 ttcctcgact attacgcaac cggaaaactg gatgttgata ccgcttcagc ggtgatcagc
    14341 ggcatcgcgg aaggttgtct gcaatcgggc tgttcactgg tgggtggcga aacggcagaa
    14401 atgccgggga tgtatcacgg tgaagattac gatgtcgcgg gtttctgcgt gggcgtggta
    14461 gaaaaatcag aaatcatcga cggctctaaa gtcagcgacg gcgatgttct gattgcactc
    14521 ggttccagcg gtccgcactc gaacggctat tcgctggtgc gcaaaattct tgaagtcagc
    14581 ggttgtgatc cgcaaaccac cgaacttgat ggtaagccat tagccgatca tctgctggca
    14641 ccgacccgca tttacgtgaa gtcagtgctg gagttgattg aaaaggtcga tgtgcatgcc
    14701 attgcgcacc tgaccggcgg cggcttctgg gaaaacattc cacgcgtatt gccagataat
    14761 actcaggcag tgattgatga atcttcctgg cagtggccgg aagtgttcaa ctggctgcaa
    14821 acggcaggta acgttgagcg ccatgaaatg tatcgcacct tcaactgcgg cgtcgggatg
    14881 attattgccc tgcctgctcc ggaagtggac aaagccctcg ccctgctcaa tgccaacggt
    14941 gaaaacgcgt ggaaaatcgg tatcatcaaa gcctctgatt ccgaacaacg cgtggttatc
    15001 gaataatgaa tattgtggtg cttatttccg gtaacggaag taatttacag gcaattattg
    15061 acgcctgtaa aaccaacaaa attaaaggca ccgtacgggc agttttcagc aataaggccg
    15121 acgcgttcgg ccttgaacgc gcccgccagg cgggtattgc aacgcatacg ctcatcgcca
    15181 gcgcgtttga cagtcgtgaa gcctatgacc gggagttgat tcatgaaatc gacatgtacg
    15241 cacccgatgt ggtcgtgctg gctggtttta tgcgcattct cagcccggcg tttgtctccc
    15301 actatgccgg gcgtttgctg aacattcacc cttctctgct gccgaaatat cccggattac
    15361 acacccatcg tcaggcgctg gaaaatggcg atgaagagca cggtacatcg gtgcatttcg
    15421 tcaccgatga actggacggt ggcccggtta ttttacaggc gaaagtcccg gtatttgctg
    15481 gtgatacgga agatgacgtc accgcccgcg tgcaaaccca ggaacacgct atttatccac
    15541 tggtgattag ctggtttgcc gatggtcgtc tgaaaataca cgaaaacgcc gcgtggctgg
    15601 atggtcaacg tctgccgccg cagggctacg ctgccgacga gtaatgcccc cgtagttaaa
    15661 gcgccagctc tgccgctggc gtttttcaat tcacctgtaa atcgcaagct ccagcagttt
    15721 ttttccccct tttctggcat agttggacat ctgccaatat tgctcgccat aatatccagg
    15781 cagtgtcccg tgaataaaac ggagtaaaag tggtaatggg tcaggaaaag ctatacatcg
    15841 aaaaagagct cagttggtta tcgttcaatg aacgcgtgct tcaggaagcg gcggacaaat
    15901 ctaacccgct gattgaaagg atgcgtttcc tggggatcta ttccaataac cttgatgagt
    15961 tctataaagt ccgcttcgct gaactgaagc gacgcatcat tattagcgaa gaacaaggct
    16021 ccaactctca ttcccgccat ttactgggca aaattcagtc ccgggtgctg aaagccgatc
    16081 aggaattcga cggcctctac aacgagctat tgctggagat ggcgcgcaac cagatcttcc
    16141 tgattaatga acgccagctc tccgtcaatc aacaaaactg gcttcgtcat tattttaagc
    16201 agtatctgcg tcagcacatt acgccgattt taatcaatcc tgacactgac ttagtgcagt
    16261 tcctgaagga tgattacacc tatctggcgg tggaaattat ccgtggagac accatccgtt
    16321 acgcgctgct ggagatccca tcagataaag tgccgcgctt tgtgaattta ccgccagaag
    16381 cgccgcgtcg acgcaagccg atgattcttc tggataacat tctgcgttac tgccttgatg
    16441 atattttcaa aggcttcttt gattatgacg cgctgaatgc ctattcaatg aagatgaccc
    16501 gcgatgccga atacgattta gtgcatgaga tggaagccag cctgatggag ttgatgtctt
    16561 ccagtctcaa gcagcgttta actgctgagc cggtgcgttt tgtttatcag cgcgatatgc
    16621 ccaatgcgct ggttgaagtg ttacgcgaaa aactgactat ttcccgctac gactccatcg
    16681 tccccggcgg tcgttatcat aattttaaag actttattaa tttccccaat gtcggcaaag
    16741 ccaatctggt gaacaaacca ctgccgcgtt tacgccacat ttggtttgat aaagcccagt
    16801 tccgcaatgg ttttgatgcc attcgcgaac gcgatgtgtt gctctattat ccttatcaca
    16861 cctttgagca tgtgctggaa ctgctgcgtc aggcttcgtt cgacccgagc gtactggcga
    16921 ttaaaattaa catttaccgc gtggcgaaag attcacgcat catcgactcg atgatccacg
    16981 ccgcacataa cggtaagaaa gtcactgtgg tggttgagtt acaggcgcgt ttcgacgaag
    17041 aagccaacat tcactgggcg aagcgcctga ccgaagcagg cgtgcacgtt atcttctctg
    17101 cgccggggct gaaaattcac gccaaactgt tcctgatttc acgtaaagaa aacggtgaag
    17161 tggtccgtta cgcacacatc gggaccggga actttaacga aaaaaccgcg cgtctttata
    17221 ctgactattc gttgctgacc gcagatgcgc gtatcaccaa cgaagtacgg cgggtattta
    17281 actttattga aaacccatac cgcccagtga catttgatta tttaatggtg tcaccgcaaa
    17341 actctcgccg tctgttatat gaaatggtag accgcgaaat cgccaacgcg cagcaagggc
    17401 tgcccagtgg tatcaccctg aagctaaata accttgtcga taaaggcctg gttgatcgtc
    17461 tgtatgcggc ctccagctcc ggcgtaccgg ttaatctgct ggttcgcgga atgtgttcgc
    17521 tgatccccaa tccggaaggc attagcgaca acattcgtgc catcagtatt gttgaccgtt
    17581 accttgaaca tgaccgggtt tatatttttg aaaatggcgg cgataaaaag gtctaccttt
    17641 cttccgccga ctggatgacg cgcaatattg attatcgtat tgaagtggcg acaccgctgc
    17701 tcgatccgca cctgaagcag cgggtgctgg acatcatcga catattgttc agcgatacgg
    17761 tcaaagcacg ttatatcgat aaagaactca gtaatcgcta cgttccccgc ggcaatcgcc
    17821 gcaaagtacg ggcgcagttg gcgatttacg actacatcaa atcactcgaa caacctgaat
    17881 aaccctatgc caatacacga taaatcccct cgtccgcagg agtttgctgc ggtcgatctt
    17941 ggttcaaaca gttttcacat ggtcatagcc cgtgtggtag atggtgccat gcagattatt
    18001 ggccgcctga aacagcgagt gcatctggcg gacggcctgg ggccagataa tatgttgagt
    18061 gaagaggcaa tgacgcgcgg tttgaactgt ctgtcgctgt ttgccgaacg gctacaaggg
    18121 ttttctcctg ccagcgtctg tatagtcggc acccatacgc tgcgtcaggc gctgaacgcc
    18181 actgactttc tgaaacgcgt ggaaaaggtc attccctacc cgattgaaat tatttccggt
    18241 aatgaagaag cccgtctgat ttttatgggc gtggaacata cccaaccgga gaaaggtcgc
    18301 aaactggtta ttgatattgg cggcggatct acggaactgg tgattggtga aaatttcgaa
    18361 ccgattctcg ttgaaagccg ccggatgggt tgtgtcagct ttgcccagct ttacttccct
    18421 ggcggggtca tcaataaaga gaattttcag cgcgctcgta tggcggcagc acaaaaactg
    18481 gaaactttaa cctggcaatt ccgtattcag ggctggaacg tggcaatggg cgcttccggt
    18541 accataaaag ccgcccatga agtgttaatg gaaatgggcg agaaagacgg gataattacc
    18601 ccggaacgtc tggaaaaact ggtaaaagaa gttttacgtc accgtaattt cgcatcgctg
    18661 agtttaccgg gtctttccga agagcggaaa acagtcttcg ttcctggact ggcgatttta
    18721 tgcggtgtgt ttgatgcttt agccatccgt gaactgcgcc tttctgacgg ggcgttacgc
    18781 gaaggcgtac tgtacgaaat ggaaggacgt ttccgtcatc aggatgtgcg tagtcgcacc
    18841 gccagcagcc tcgccaacca gtatcacatc gacagcgaac aggcccgacg ggtgctggat
    18901 accactatgc aaatgtacga acagtggcgg gaacagcaac ctaagctggc gcatccgcaa
    18961 ctggaggcgc tactgcgatg ggccgccatg ctgcatgagg tcgggttgaa tatcaaccac
    19021 agcggtttgc atcgccactc cgcttatatt ctgcaaaaca gtgacttgcc gggttttaat
    19081 caggaacagc agctgatgat ggcgacactg gtgcgctatc accgcaaagc gattaaactc
    19141 gacgatctgc cgcgctttac cttgtttaag aagaaacagt tcctgccact gattcagcta
    19201 ttgcgccttg gcgtattact caacaatcaa cgtcaggcaa ccaccacacc gccaacattg
    19261 acactgatta ctgatgacag tcactggaca ctgcgtttcc cgcatgactg gtttagtcag
    19321 aatgcgttgg tactgcttga tctggaaaag gagcaagaat actgggaagg cgtggctggc
    19381 tggcggttga aaattgaaga agaaagtaca cctgaaatcg ccgcttaatg ctttgcgggc
    19441 cgacgagaaa tgtcggcccg cattattcag gcactttcgc gaatgggtt
//
LOCUS       DAJTNN010000069        19482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTNN010000069 DAJTNN010000000
VERSION     DAJTNN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.069"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            49..993
                     /gene="wecH"
                     /locus_tag="OO150_002115"
     CDS             49..993
                     /gene="wecH"
                     /locus_tag="OO150_002115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5516261.1"
                     /translation="MVVMIHTTTWYVTNAHSVSPVTWDIANVLNSASRVSVPLFFMIS
                     GYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINMELALKNLLQKPVFYHLW
                     FFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVPQKIDGFEWLPINLYIN
                     GDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIISRGTLYELQWRGNFADT
                     WYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGIYGFHALIIHALRTRGI
                     ELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(1035..1472)
                     /locus_tag="OO150_002116"
     CDS             complement(1035..1472)
                     /locus_tag="OO150_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5516262.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(1518..1859)
                     /locus_tag="OO150_002117"
     CDS             complement(1518..1859)
                     /locus_tag="OO150_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516263.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(2024..2215)
                     /locus_tag="OO150_002118"
     CDS             complement(2024..2215)
                     /locus_tag="OO150_002118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516264.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(2148..3476)
                     /gene="xylB"
                     /locus_tag="OO150_002119"
     CDS             complement(2148..3476)
                     /gene="xylB"
                     /locus_tag="OO150_002119"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5516265.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGCGASTGRSKAGADCGESREIAH"
     gene            complement(3593..4870)
                     /gene="xylA"
                     /locus_tag="OO150_002120"
     CDS             complement(3593..4870)
                     /gene="xylA"
                     /locus_tag="OO150_002120"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR5516266.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVSFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR"
     gene            5236..6207
                     /gene="xylF"
                     /locus_tag="OO150_002121"
     CDS             5236..6207
                     /gene="xylF"
                     /locus_tag="OO150_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5516267.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGG
                     AIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGN
                     GQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6285..7826
                     /gene="xylG"
                     /locus_tag="OO150_002122"
     CDS             6285..7826
                     /gene="xylG"
                     /locus_tag="OO150_002122"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5516268.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            7804..8984
                     /gene="xylH"
                     /locus_tag="OO150_002123"
                     /pseudo
     CDS             7804..8984
                     /gene="xylH"
                     /locus_tag="OO150_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
     gene            9062..10240
                     /gene="xylR"
                     /locus_tag="OO150_002124"
     CDS             9062..10240
                     /gene="xylR"
                     /locus_tag="OO150_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCR5516269.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNREVML
                     "
     gene            complement(10348..11172)
                     /locus_tag="OO150_002125"
     CDS             complement(10348..11172)
                     /locus_tag="OO150_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5516270.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            11492..13522
                     /gene="malS"
                     /locus_tag="OO150_002126"
     CDS             11492..13522
                     /gene="malS"
                     /locus_tag="OO150_002126"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5516271.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            13700..14953
                     /gene="avtA"
                     /locus_tag="OO150_002127"
     CDS             13700..14953
                     /gene="avtA"
                     /locus_tag="OO150_002127"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5516272.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNVFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(15104..15577)
                     /locus_tag="OO150_002128"
     CDS             complement(15104..15577)
                     /locus_tag="OO150_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5516273.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(15679..16527)
                     /gene="yiaJ"
                     /locus_tag="OO150_002129"
     CDS             complement(15679..16527)
                     /gene="yiaJ"
                     /locus_tag="OO150_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5516274.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            16728..17726
                     /gene="yiaK"
                     /locus_tag="OO150_002130"
     CDS             16728..17726
                     /gene="yiaK"
                     /locus_tag="OO150_002130"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5516275.1"
                     /translation="MKVTFEQLKAAFNRALISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            17738..18206
                     /locus_tag="OO150_002131"
                     /pseudo
     CDS             17738..18206
                     /locus_tag="OO150_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            18324..18797
                     /gene="yiaM"
                     /locus_tag="OO150_002132"
     CDS             18324..18797
                     /gene="yiaM"
                     /locus_tag="OO150_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCR5516276.1"
                     /translation="MKKILEVILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            18800..>19482
                     /locus_tag="OO150_002133"
     CDS             18800..>19482
                     /locus_tag="OO150_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5516277.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPDMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIG"
BASE COUNT         4944 a         4874 c         4729 g         4935 t
ORIGIN      
        1 cagcccaaaa tttactggat tgataacctg cgagggatag cgtgtttaat ggtggtgatg
       61 attcacacca ctacctggta tgtgaccaat gctcatagtg ttagccccgt cacatgggat
      121 atcgccaatg ttctgaattc tgcctctcgt gtcagcgtgc cgctattttt catgatttcc
      181 ggctatctct tttttggcga acgcagcgcc cagccgcgcc atttcttgcg tatcggctta
      241 tgtctgattt tttatagcgc aatcgcactg ctctacattg cgctgtttac ctccatcaat
      301 atggagttag cgctgaaaaa cctgctgcaa aagccagtgt tttaccactt gtggtttttc
      361 ttcgcgattg cggtgattta tctggtttca ccgctgattc aggtgaagaa cgtcggcgga
      421 aaaatgttgc tggtgctaat ggcggtgatt ggcattatcg ctaacccaaa cacagtgccg
      481 cagaaaattg acggttttga atggctgcca attaacttat atatcaatgg cgatactttt
      541 tactacattc tgtatggcat gttgggccgc gctataggga tgatggacac acagcataaa
      601 gcactgtcgt gggtgagcgc cgcgctgttt gcgacggggg tttttattat ctctcgcggg
      661 acattatatg aattgcagtg gcgcggaaat tttgccgata cctggtatct ttactgtggg
      721 ccgatggttt ttatctgcgc aatcgcgcta ttgactctgg ttaaaaacac gctggatacg
      781 cgtaccattc gcggacttgg cttaatctcc cgccattcat tgggtatata cggattccac
      841 gccttgatta tccatgcgct gcgcacccgg ggaattgagc ttaaaaattg gccaatactg
      901 gatattattt ggatcttttg cgcgacgttg gcagcgagtt tgttactttc tatgctggta
      961 caacgaatcg acagaaacag attagtgagt taagtaaaag cccgttcaca ttggactgac
     1021 cgggcttacg tgaattattc gctgtattct tcctgggtaa cctgtgtttc ttttggcggg
     1081 tttattccgg catcacgaat attcttctgc accgctactg caccaaacaa gcttaagaag
     1141 aaagccagtc cataaaaacc tttttcactg agtaataatg tcgcattcca cagacctacc
     1201 atcagtaatg caacagagat aataaagaca gtcaggcagg tcatataata aatggaagtg
     1261 gtcggtatgc cttcatactt gtcccgaacg gtcttttgat aagacgcagc agaaaacagt
     1321 cctaacacca gtacggcaaa ataataccct ttttcattta actgcatctc tgcattccat
     1381 agccctaaca gataggtaac gataccacca acgagagcta tccatgacac aatactaaag
     1441 gccggtgaat aggttgatat tttgttgtcc atcacagtat tcctttcatt cctgaatgtg
     1501 taagagcttt atgttgctca taccgagcga aacaaacgca tttgaccaaa caagcagaca
     1561 aaaaaggcga tggggtagat caccatttca taagcattga taggtgcatt taaaacaccg
     1621 ataaacaaga gaccgatagt gattaacgca actaactggc agatatgggt aaaaaaatta
     1681 tccagttgcc cgagtttttc tgcgcgaaga tatgcatagt taccaaaagt tgctgtcatt
     1741 aacacgccaa gaaaatagcc ttttccactt agcaatgggc atgagatcca tagcccaacc
     1801 agataaacca gcgccccgac aacaaataat agttttgcca cagtttttga ggttttcata
     1861 acgatctcca tatctaccag cgatacatta cgagtaacca acgaaagaca aaactgaaaa
     1921 atgccattaa caaatgattt tcagaataaa ttcatactaa atattaatta gtgagatata
     1981 tagatgtgaa ttatccccca cccggtcagg caggggataa cgtttacgcc attaatggca
     2041 gaagttgctg atagaggcga cggaacgttt cacgtcgtgg ctgataagcg gcataacgct
     2101 gcgcatctgg tagatgcgac tgttctaacg gtagctgcgg caacaactca atgagcgatt
     2161 tctctggatt cgccgcaatc tgcgccagcc ttgctgcgcc cagtgctggc cccacatccc
     2221 cccgtacggt aatcgagctg ctgaccgctg atatccgcca gcatctgacg ccagtactca
     2281 ctacgcgccc cgccaccaat caacgtaaca ctttgcggtt taatgccgca ggcatgcacg
     2341 acatccatgc catctgccag cgcataaccc acgccttcca gcactgctcg cgccagttca
     2401 ttggggccat gttgatgagt caaaccaaag aaaaccccct tcgcctgggg attattgtgt
     2461 ggtgtacgct cgccggaaag ataaggcaga aaccaaactg gctcggcact ttcatcagcc
     2521 tgttgagctg cagcgattaa agctgggaca ttgcacaggc cggttaattt cgcgacccaa
     2581 tccagacacg atgctgcact cagcattaca gacattaaat gccaacgctg cggtagcgca
     2641 tggcaaaagc tgtgtacagc gctttctggc ttgcttaaga acccttcgct gacagcaaaa
     2701 tagacccccg acgtccccag cgataacatt gcctgattag catcaaccat tcccacacca
     2761 actgcaccag ctgcattgtc gccaccgcct gcgacaactg gcaccgtcgc cataccccac
     2821 gctttcgcaa cttcaggtaa caaagcacca gtaatttcgc tgccttcgta taatgcgggc
     2881 atctggtcac gagataagtc gcaagcctgc agcatgacgt cactccagtc acgctttgcg
     2941 acatccagcc acatggtgcc agctgcgtca gacatatcgc tggcaaactc ccccgtcata
     3001 cgcagacgca agtaatcttt cggtaataat actttgtcga tttgacggaa tatctccggc
     3061 tcatgccgct gaacccatag caatttaggc gcagtaaatc cgggcatcat caggttgccg
     3121 gtaatcactc gtgattgcgg aactctcgct tccagcaaag tgcactcttg cgcacagcgc
     3181 ccgtcgttcc acaaaatggc agggcgcaat acccgttgtt gagcatccag taaggttgct
     3241 ccatgcatct ggccggcaat acccaatgct ttaacgtcct gcagagaatg ctgatcgccc
     3301 agagctttca ttgcgcgatc agttgcctgc caccactgtt ccgggtcttg ttccgaccag
     3361 agtggatgcg ggcgcgaaac ggtcagcttt tccgtttgcg aagcaaccac ctcaccctgc
     3421 tcgttgagca aaataacttt tacgcccgag gtgccaagat ctatcccgat atacatatcg
     3481 atcgttcctt aaaaaaatgc ccggtatcgc taccgataac cgggccaacg gactgcacag
     3541 ttagccgtta tttgtcgaac agataatgat ttaccagatt ttccagttgc tcctagcgac
     3601 cactctgatg caccggagac aaattatgtt cctgagcata tttggctaaa tctgccagtg
     3661 acatttggcc tttcaggatt tgctggccca attcgctatt ccagccggaa taacgctgcg
     3721 cgatgcgttt atccagctcg ccatcttcaa tcatgcgcgc tgcaattttc agcgccagtg
     3781 ccatcgtatc catcgcgccg atatgaccgt aaaacagatc atatttatca gtactttgac
     3841 gacgtacttt ggcatcgaag ttcagaccac cggtggtgaa accgcctgct ttgagaattt
     3901 catacatcac cagcgcattc tcttccacac tgttcgggaa ctggtcggtg tcccagccca
     3961 gttgcgcatc gccacggttg gcgtcgacag aaccgaacag gccaagcgca atggcggtgg
     4021 ctatttcatg atggaaagag tgacctgcca gcgtcgcgtg gttagcttca atgttcagtt
     4081 taatctcttt ttccagacca aactgtttca ggaagccata gaccgtcgcg gcatcgtaat
     4141 catattgatg tttagtcggt tcttgcggtt tcggttcgat aagcaacgtg ccctggaagc
     4201 cgattttatg tttatgctca accaccatct gcataaagcg gcccagttgt tcacgctcct
     4261 gacgcaagtc ggtatttaac agcgtttcgt aaccttcacg accaccccac aggacatagt
     4321 tttcaccgcc caatttatgg gttgcttcca tcgctgtaac aacttgcgtt gccgcccagc
     4381 tgaagacttc aggatctggg ttcgtcgccg cacccgcgcc atagcgaggg tttgtaaagc
     4441 agttagcggt tccccacagc agcttcacgc cgctctcttc ttgcttggct gccaggacat
     4501 caaccatttg cgcaaaatta ttgatgtact cttttaacga cgcgccctca ggggaaacat
     4561 ccacatcgtg gaagcaataa aatgacacat gtaacttgtg gaaaaactca aatgcgacat
     4621 ctgctttacg cttcgccaac gccagtgcct caccaggctg ctgccacgga cgattaaacg
     4681 cccccacacc aaacatgtcc gccccgttcc agcagaaggt gtgccagtaa caggcggcaa
     4741 aacgcaagtg ctcttccata cgcttaccca acaccagttc gtcgggattg tagtgacgga
     4801 atgctaacgg gtttgaggat tttgagcctt cataacgaac gcgatcgagc tggtcaaaat
     4861 aggcttgcat attgaactcc ataatcaggt aatgccgcgg gtgatggatg atgtcgtaat
     4921 attgggcact ccctttcagt tgctcaatta tgttatttca cactgctatt gagataattc
     4981 acaagtgtgc gctcgctcgc aaaataaaat gggatgatga aactgggtaa ttcctcgaag
     5041 ataaaaatgc aataagtaca attgcgcaac aaaagtaaga tctcggtcat aaatcaagaa
     5101 ataaaccaaa aatcgtaatc gaaagataaa aatctgtaat tgttttcccc tgtttagttg
     5161 ctaaaaattg gttacgttta tcgcggtgat tgttacttat taaagctgtc ctctaactac
     5221 agaaggccct acaccatgaa aataaagaac attctactca ccctttgcac ctcacttctg
     5281 cttaccaacg ttgcggcaca cgccaaagaa gtcaaaatag gtatggcgat tgatgatcta
     5341 cgtcttgaac gctggcaaaa agatcgagat atttttgtga aaaaggctga atctctcggt
     5401 gcgaaagtat ttgtacagtc tgcaaatggc aatggagaaa cacaaatgtc gcagattgaa
     5461 aacatgatta accggggtgt cgatgttctt gtcattattc catataacgg ccaggtatta
     5521 agtaacgttg taaaagaagc caaacaagaa ggtattaaag tattagctta cgaccgtatg
     5581 attaacgatg cggatatcga tttttatatt tctttcgata acgaaaaagt cggtgaactg
     5641 caggcaaaag ccctggtcga tattgttccg caaggtaatt acttcctgat gggcggctcg
     5701 ccggtagata acaacgccaa gctgttccgc gccggacaaa tgaaagtgtt aaaaccttac
     5761 gttgattccg gaaaaattaa agtcgttggt gaccaatggg ttgatggctg gttaccggaa
     5821 aacgcgctaa ccgccaataa taacaaaatt gatgctgtag ttgcctcaaa cgatgccacc
     5881 gcaggtgggg caattcaggc attaagcgcg caaggtttat cagggaaagt agcaatttcc
     5941 ggccaggatg cggatctcgc aggtattaaa cgtattgctg ccggtacgca aactatgacg
     6001 gtgtataaac ctattacatt gctggcaaat actgccgcag aaattgccgt tgaattgggc
     6061 aatggtcagg agccaaaagc agatacctca ctgaataatg gcctgaaaga tgtcccctcc
     6121 cgcctcctga caccgatcga tgtgaataaa aacaacatca aagatacggt aattaaagac
     6181 ggattccaca aagagagcga gctgtaagcg ttacgccccg gcgcggagcg ggggcgtgat
     6241 ttctctccat gccgcgtgaa tgaattggct taggtggagt cgttatgcct tatctacttg
     6301 aaatgaagaa cattaccaaa accttcggca gtgtgaaggc gattgataac gtcagcttgc
     6361 ggttgaatgc tggcgaaatc gtctcactct gtggggaaaa tgggtctggt aaatcaacgc
     6421 tgatgaaagt gctgtgtggt atttatcccc atggctccta cgaaggcgaa attatttttg
     6481 cgggagaaga gattcaggcg agtcacatcc gcgataccga acgcaaaggt atcgccatca
     6541 ttcaccagga attggccctg gtgaaagaat tgaccgtgct ggaaaatatc ttcctgggta
     6601 acgaaataac ccacaatggc attatggatt atgacctgat gacgctacgc tgtcagaagc
     6661 tgctcgcaca ggtcagttta tccatttcac ctgatacccg cgttggcgat ttagggcttg
     6721 ggcaacaaca actggttgaa attgccaagg cacttaataa acaggtgcgc ttgttaattc
     6781 tcgatgaacc gacagcctca ttaactgagc aggaaacgtc ggttttactg gatattattc
     6841 gcgatctaca acagcacggt atcgcctgta tttatatttc gcacaaactc aacgaagtca
     6901 aagcgatttc cgatacgatt tgcgttattc gcgacggtca gcacattggt acgcgtgatg
     6961 ctgccggaat gagtgaagac gatattatca ccatgatggt cgggcgagag ttaaccgcgc
     7021 tttaccctaa tgaaccacat accaccggag atgaaatatt acgtattgaa catctgacgg
     7081 catggcatcc ggttaatcgt catattaaac gagttaatga tgtctcgttt tccctgaaac
     7141 gtggcgaaat actgggtatt gccggactcg ttggtgccgg acgtaccgag accattcagt
     7201 gcctgtttgg cgtgtggccc ggacaatggg aaggaaaaat ttatattgat ggcaaacagg
     7261 tagatattcg taactgtcag caagccatcg cccaggggat tgcgatggta cccgaagaca
     7321 gaaagcgcga cggcatcgtt ccggtaatgg cggttggtaa aaatattacc ctcgccgcac
     7381 tcaataaatt taccggtggc attagccagc ttgatgacgc ggcagagcaa aaatgtattc
     7441 tggaatcaat ccagcaactc aaagttaaaa cgtcgtcccc cgatcttgct attggacgtt
     7501 tgagcggcgg caatcagcaa aaagcgatcc tcgcccgctg tctgttactc aacccgcgca
     7561 ttctcattct tgatgaacct accaggggta ttgatattgg cgcgaaatat gagatctaca
     7621 aattaattaa ccaactcgtc cagcagggta ttgccgttat tgtcatctct tccgaattac
     7681 ctgaagtgct cggccttagc gatcgtgtac tggtgatgca tgaagggaaa ctaaaagcca
     7741 acctgataaa tcataacctg actcaggagc aggtgatgga agccgcattg aggagcgaac
     7801 atcatgtcga aaagcaatcc gtctgaagtg aaattggccg taccgacatc cggtggcttt
     7861 tccgggctga aatcactgaa tttgcaggtc ttcgtgatga ttgcagctat catcgcaatc
     7921 atgctgttct ttacctggac caccgatggt gcctacttaa gcgcccgtaa cgtctccaac
     7981 ctgttacgcc agaccgcgat taccggcatc ctcgcggtag gaatggtgtt cgtcataatt
     8041 tctgctgaaa tcgacctttc cgtcggctca atgatggggc tattaggtgg cgtcgcggcg
     8101 atttgtgacg tctggttagg ctggcctttg ccacttacca tcattgtgac gttggttctg
     8161 ggactgcttc tcggtgcgtg gaacggatgg tgggtcgcgt accgtaaagt cccttcattt
     8221 attgtcaccc tcgcgggcat gttggcattt cgcggcatac tcattggcat caccaacggc
     8281 acgactgtat cccccaccag cgccgcgatg tcacaaattg ggcaaagcta tctccccgcc
     8341 agtaccggct tcatcattgg ccgcttggct taatggcttt tgttggttgg caatggcgcg
     8401 gaagaatgcg ccgtcaggct ttgggtttac agtctccggc ctctaccgca gtagtcggtc
     8461 gccaggcttt aaccgctatc atcgtattag gcgcaatctg gctgttgaat gattaccgtg
     8521 gcgttcccac tcctgttctg ctgctgacgt tgctgttact cggcggaatg tttatggcaa
     8581 cgcggacggc atttggacga cgcatttatg ccatcggcgg caatctggaa gcagcacgtc
     8641 tctccgggat taacgttgaa cgcaccaaac ttgccgtgtt cgcgattaac ggattaatgg
     8701 tagccatcgc cggattaatc cttagttctc gacttggcgc tggttcacct tctgcgggaa
     8761 atatcgccga actggacgct attgcagcat gcgtgattgg cggcaccagc ctggctggcg
     8821 gtgtgggaag cgttgccgga gcagtaatgg gggcatttat catggcttca ctggataacg
     8881 gcatgagtat gatggatgta ccgaccttct ggcagtatat cgttaaaggt gcgattctgt
     8941 tgctggcagt atggatggac tccgcaacca aacgccgttc ttgattttga taaaaatttt
     9001 ctcaaagccg gttacgtatt accggttttg agtttttgca tgattcagca ggaaaagaac
     9061 catgtttact aaacgtcacc gcatcacatt actgttcaat gccaataaag cctatgaccg
     9121 gcaggtagta gaaggcgtag gggaatattt acaggcgtca caatcagaat gggatatttt
     9181 cattgaagaa gatttccgcg cccgcattga taaaatcaag gactggttag gagatggcgt
     9241 cattgccgac ttcgacgaca aacaaatcga gcaagcgctg gctgatgtcg acgtccccat
     9301 tgttggggtt ggcggctcgt atcaccttgc agaaagttac ccacccgttc attacattgc
     9361 caccgataac tatgcgctgg ttgaaagcgc atttttgcat ttaaaagaga aaggcgttaa
     9421 ccgctttgct ttttatggtc ttccggaatc aagcggcaaa cgttgggcca ctgaacgcga
     9481 atatgcattt cgtcagcttg tcgccgaaga aaagtatcgc ggagtggttt atcaggggtt
     9541 agaaaccgcg ccagagaact ggcaacacgc gcaaaatcgg ctggcagact ggctacaaac
     9601 gctgccaccg caaaccggga ttattgccgt tactgacgcc cgggcgcggc atattctgca
     9661 agtatgtgaa catctacaca ttcccgtacc ggaaaaatta tgcgtgattg gcatcgacaa
     9721 cgaagaactg acccgctatc tgtcgcgtgt cgccctttct tcggtcgctc agggcgcgcg
     9781 gcaaatgggc tatcaggcgg caaaactgtt gcatcgatta ttagataaag aagaaatgcc
     9841 gctacagcga attttggtcc caccagttcg cgtcattgaa cggcgctcaa cagattatcg
     9901 ctcgctgacc gatcccgccg ttattcaggc catgcattac attcgtaatc acgcctgtaa
     9961 agggattaaa gtggatcagg tactggatgc ggtcgggatc tcgcgctcca atcttgagaa
    10021 gcgttttaaa gaagaggtgg gtgaaaccat ccatgccatg attcatgccg agaagctgga
    10081 gaaagcgcgc agtctgctga tttcaaccac cttgtcgatc aatgagatat cgcaaatgtg
    10141 cggttatcca tcgctgcaat atttctactc tgtttttaaa aaagcatatg acacaacgcc
    10201 aaaagagtat cgcgatgtaa atcgtgaggt catgttgtag cgatgatgag aattgtcggc
    10261 gtcacatcag ggaactcagc acagccacct gatgcgagca aaagccttat ccgaccgaca
    10321 aatcagataa ggctcaagga aatgcaatta catatgcgcc gcgattaacc gttggttatc
    10381 ctggtacatt gcgaacaggt agttattata actcttccct ttggtcgagt agcccttcag
    10441 cttgtgaatc atcgctgtgg cagtcacttc ctgatccgct ttacgcagct gcgcacgcga
    10501 tttacggaac gaagagtaag ccgggtgcgt attcaggtta gtgacatagg cgctcaccga
    10561 ttctttgaca gaactaaact gtgagtaccc tttcacttta cccggcgcat tggtacaacg
    10621 tcctttcatg catttcatgc cgaacagatt gttattgttg cgcgccagct tcgacgttcc
    10681 ccaaccgctt tctgctgcag ccatcgtcgc caccatactg gtagggataa tgtctacgcg
    10741 ttcaagcaag gtattccacg ggatttttcg cgtattacca gaccacttca ccttgtagcg
    10801 tttggcgatg tctttcagac gcgcacgctc agcaggtgac cattggccct gatactgttt
    10861 tgaaatcagc cagttacgtt ctgcagtaat ggctgcattt tggctggtaa tgtaaggcat
    10921 tacggtccgg agaaacgcct ttttccttgg tgttccggaa gggtattttc gcaaatcagg
    10981 aagtgaacta ctctttgcac tattgcgaga atactcttgt ttactgctta cctgtttatt
    11041 actagcttta gttaagtggg acttttgact cgctgttgtt gtgtgcgtct tcgctaacac
    11101 ctcactcgaa aacaccagag tgagtaacat aagaatcatc gccccatatc gtcgtatggg
    11161 agtcaaaatc atcaggtctc ctggtcggat ttaatcattc caacacctta tatttttcac
    11221 aaatttgaga gttgaatctc aaatcatatc aaaaatagct gtcaagagca ccccaaggaa
    11281 tagtccaaat ctgaaactat gtcacgagtt aacgattcag attggcgcta aatcgcagaa
    11341 aatgtggggt ttatcgcaaa attcagccgt tttttgcgcg cgatcgctca cccttgcttc
    11401 tcatcctgtg gacttaccgc tcagggatga gttttgtctg gcttatcgct ggcaaactgt
    11461 ctgaaatcgc agcaataagg actcatccac catgaaactc gcctcctgtt ttctgacact
    11521 ccttcctggc ttcgccgttg ccgccagctg gacttctccg gggttccctg cctttagcga
    11581 acagggaacg ggaacatttg tcagccacgc gcagttgccc aaaggtacgc gtccactcac
    11641 gctaaatttt gaccaacagt gctggcagcc tgcagatgcg ataaaactca atcagatgct
    11701 ttccctgcaa ccttgtagca acacgccgcc tcaatggcga ttgttcaggg acggcaaata
    11761 tacgctgcaa atagacaccc gctccggtac gccaacattg atgatttcca tccagaacgc
    11821 cgccgaaccg gtagcaaacc tggtccgtga atgcccgaaa tgggatggat taccgctcac
    11881 gctggatgtc agcgccactt tcccggaagg agccgccgta cgggattatt acagccagca
    11941 aattgcgata gtgaagaacg gtcaaataac gttacaaccc gctgctacca gcaacggttt
    12001 actcctgctg gaacgggcag aaactgacac atccgcccct ttcgactggc ataacgccac
    12061 ggtttacttt gtgctgacag atcgtttcga aaacggcgat cccagtaatg accagagtta
    12121 cggacgtcat aaagacggta tggcggaaat tggcactttt cacggcggcg atttacgcgg
    12181 cctgatcaac aaactggatt acctccagca gttgggcgtt aatgctttat ggataagcgc
    12241 cccatttgag caaattcacg gctgggtcgg cggcggtaca aaaggcgatt tcccgcatta
    12301 tgcctaccac ggttattaca cacaggactg gacgaatctt gatgccaata tgggcaacga
    12361 agccgatcta cggacgctgg ttgatagcgc acatcagcgc ggtattcgta ttctctttga
    12421 tgtcgtgatg aaccacaccg gctatgccac gctggcggat atgcaggagt atcagtttgg
    12481 cgcgttatat ctttctggtg acgaagtgaa aaaaacgctg ggtgaacgct ggagcgactg
    12541 gaaacctgcc gccgggcaaa cctggcatag ctttaacgat tacattaatt tcagcgacaa
    12601 aacaggctgg gataaatggt ggggaaaaaa ctggatcaga acggatatcg gcgattacga
    12661 caatcctgga ttcgacgatc tcactatgtc gctggccttt ttgccggata tcaaaaccga
    12721 atcaactacc gcttctggtc tgccggtgtt ctataaaaac aaaacggata ctcacgctaa
    12781 agtcatcgaa ggctttacac ctcgcgatta cttaacccac tggttaagtc agtgggtccg
    12841 cgactatggg attgatggtt ttcgggtcga taccgccaaa catgttgagt tgcccgcctg
    12901 gcagcaactg aaaaccgaag ccagctccgc gcttcgcgaa tggaaaaaaa ctaaccccga
    12961 caaagcatta gatgacaaac ctttctggat gaccggtgaa gcctggggcc acggcgtgat
    13021 gcaaagtgac tactatcgcc acggcttcga tgcgatgatc aatttcgatt atcaggagca
    13081 ggcggcgaaa gcagtcgatt gtctggcgca gatggatacg acctggcagc aaatggcgga
    13141 gaaattgcag ggtttcaacg tgttgagcta cctctcgtcg catgataccc gtctgttccg
    13201 tgaagggggc gacaaagcag cagagttatt actattagcg ccaggcgcgg tacaaatctt
    13261 ttatggcgat gaatcctcgc gtccgttcgg tcctacaggt tctgatccgc tgcaaggtac
    13321 acgttcggat atgaactggc aggatgttag cggtaaatct gccgccaacg tcgcgcactg
    13381 gcagaaaatc agccagttcc gcgcccgcca tcccgcaatt ggcgcgggca aacaaacgac
    13441 actttcgctg aagcagggct acggctttgt tcgtgagcat ggcgacgata aagtgctggt
    13501 catctgggct gggcaacagt gacttttccg gcttcccgtt catcagtacc tcgggaagcc
    13561 gccaaccagg ataaaatgtc agccctaatc agcgttgcag gataaagcac cgctcactct
    13621 tcaacagacc gatttgcacc ccagcaaatg tagcgttatt gttaccttcc ttgctacaga
    13681 gttcgacaga tatcccgcta tgacattctc cctttttggt gacaaattta cccgccactc
    13741 cggcattacg ctgctgatgg aagatctgaa cgacggttta cgcacgcctg gcgcgattat
    13801 gctcggcggc ggtaatccgg cgcagatccc ggaaatgcag gactacttcc agacgctact
    13861 gaccgacatg ctggaaagtg gcaaagcgac tgatgcactg tgtaactacg acggtccaca
    13921 ggggaaaacg gagctactca cactgcttgc cggaatgctg cgcgagaagt tgggttggga
    13981 tatcgaacca cagaatattg cactaacaaa cggcagccag agcgcgtttt tctacttatt
    14041 taacctgttt gccggacgcc gtgccgatgg tcgggtcaaa aaagtgctgt tcccgctggc
    14101 accggaatac attggctacg ctgacgccgg actggaagaa gatctgtttg tctctgcgcg
    14161 tccgaatatt gaactgctgc cggaaggcca gtttaaatac cacgtcgatt ttgagcatct
    14221 gcatattggc gaagaaaccg ggatgatttg cgtctcccgg ccgacgaatc caacaggcaa
    14281 tgtgattact gacgaagagt tgctgaagct tgacgcgctg gcgaatcaac acggcattcc
    14341 gctggtgatt gataacgctt atggcgtccc gttcccgggt atcatcttca gtgaagcgcg
    14401 cccgctatgg aatccgaata tcgtgctgtg catgagtctt tccaagctgg ggctgcctgg
    14461 ctcccgctgc ggcattatca tcgccaatga aaaaatcatc actgccatca ccaatatgaa
    14521 cggcattatc agcctggcac ctggcggtat tggtccggcg atgatgtgtg aaatgattaa
    14581 gcgtaacgat ctgctgcgcc tgtctgaaac agtcatcaaa ccgttttact accagcgtgt
    14641 tcaggaaact atcgccatca ttcgccgcta tttaccggaa aatcgctgcc tgattcataa
    14701 accggaagga gccattttcc tctggctatg gtttaaggat ttgcccatta cgaccgagca
    14761 gctctatcag cgcctgaaag cacgcggcgt gctgatggtg ccggggcaca acgtcttccc
    14821 gggactggat aaaccgtggc cgcatacgca tcaatgtatg cgcatgaact acgtaccaga
    14881 gccggagaaa attgaggcgg gggtgaagat tctggcggaa gagatagaaa gagcctgggc
    14941 tgaaagtcac taaaaattgc ctgatgcgct acgcttatca ggcctacatg atttctgcaa
    15001 tatattgaat ttggaagaat ttgtaggccg gataaggcgt ttacgccgca tctggcataa
    15061 acaaagcgca ctattccggc gttgagaaac gccggaaaac gtttcaaaca ggctgctgcc
    15121 gtagccgatg ccgctgcact ttctcgacat ccatgcactg caacgccttt gtcgggcagg
    15181 cttcaacaca ggccggcccc gtctcccgat gccagcacag atcgcactta atcgccctcg
    15241 cctttttgcg cgaagaaacg acctccatcg caccaaacgg gcaagccagc atacagcttt
    15301 tacagccaat gcaacgtgtt tgttcaacga aaatatgccc atgttcgcgg cttatcgcgt
    15361 caacagggca gacattcgcg cacggtgcat cttcacactg atgacaggct actgccgtgg
    15421 tccagctgtg gtctttaatg acacgaatac gggaaataaa ctcgtctggt gacaacgcag
    15481 cgcaatcctg attctcctga tgcgacactg cgcaagccac ttcacaggta cggcaaccga
    15541 tacatttcgt cgcatccgca ataataaacc ggttcatgat cactcctttg cactaaaaca
    15601 gcagagtgcc agagagcgcg tccctactgc tttgatccaa cgaaggaaat gttaaagttt
    15661 tggcacttgt ccaaagcgtt atgtgatcgc gcccaggtca tccctgacag taaatcccag
    15721 ttcattagaa atagcctgcg cggtttcacg cagtggtttc aggagatttt tctctcccac
    15781 ctgtttcaga cgtgatgtcg aaagcgaaat cgacacggcg tacggcaccc gcccatgaat
    15841 atcaaacacc ggaacagcaa tacaggagac gccgagttcg ttttcttctc tgtccatcgc
    15901 cgctccgctt tcacgaatgt gcgccagttc gtcgaacatc gcgggcagct cggtaatggt
    15961 attgcgggtt aacggctgga tctcatgctg atggttttcc cagtatgact tcacgtagtc
    16021 cgggtgacca aacgccatgt agatcttgcc cattgcggaa cagtagagcg gcatatgctg
    16081 gccaatatag gcacgggttc gcagcatccc ggttgtgggt tccagcttat aaatcaaaat
    16141 agcgtgatcg tcttcgcggc tggagaagtt aatggtttca ccagtggcga tgttcagtgc
    16201 ctcaagatgc ggagcggcga tatgaatgat attcagcgaa gacagcgcct tctgcccgac
    16261 ggcaataaat ttggtagtca ggcggtaact ccctgcggcg ggcgcggtgg tcacatagcc
    16321 gcaggactgt aatccctgca ataagcgatg gacggtactc ttatttaaac cagccagctc
    16381 cgaaagatgc gccaacggac aaccgtttgg atagttgctc aaaatctcaa tcagcatcaa
    16441 cccgcgaaac agactctggc ttccggctgg acgctctttt tcctgcgcca tctcgttctc
    16501 tttttttccc atcacttctt tccccatttt gtcgcgtcct gatggtagcg caaagtgtgc
    16561 cgtagttcac gatctcgaca gataatttat aaccacttga tttttatgtc ttttgaaatt
    16621 catcaatcag attgctttgt taaaaagtga tcgatatatt tgaaatcaag tttcgcatat
    16681 tgaaatttta agccaaaaaa gcgatcaaaa aaacaaggaa gcctgggatg aaagtgacat
    16741 ttgagcagtt aaaagcagcc tttaatcggg ccttaatttc acgcggcgtt gacagcgaaa
    16801 cggctgacgc ctgtgcagag atgttcgccc gcaccaccga atccggcgtt tattctcacg
    16861 gcgttaatcg tttccctcgt ttcattcaac aactggaaaa cggcgatatc attcctgatg
    16921 cccaacccaa acgtataacc agcctcggcg caattgaaca gtgggatgcc cagcgttcga
    16981 tcggcaacct gacggcgaaa aagatgatgg atcgcgccat tgaactggct gccgatcacg
    17041 gtattggtct ggtggcactt cgtaatgcca accactggat gcgcggcggc agctacggct
    17101 ggcaggcggc ggaaaaaggc tatattggca tttgctggac caactccatc gccgtaatgc
    17161 cgccgtgggg cgcaaaagag tgtcgcattg gcactaaccc gctgatcgtc gccattcctt
    17221 ccacgccgat caccatggtc gatatgtcga tgtcgatgtt ctcttacggc atgttagaag
    17281 ttaaccgcct ggcgggccgt cagctcccgg tcgatggtgg ctttgatgat gagggcaatt
    17341 tgaccaaaga acctggcgtt atcgagaaga atcgccgcat tttgccgatg ggctactgga
    17401 aaggttctgg catgtcgatt gtgctggata tgatcgctac tctcctttcc gacggcgcat
    17461 cggttgccga agtcacccag gacaacagcg acgaatacgg catttcacaa atttttattg
    17521 ccattgaagt ggacaagctt atcgacggtc ccacccgcga tgccaagctg caacgcatca
    17581 tggattacgt tactaccgct gaacgcgctg acgaaaacca ggccatccgc ttacccggcc
    17641 acgaatttac taccctgctg gccgaaaacc gccgtaacgg catcactgtt gatgacagcg
    17701 tgtgggccaa aatccaggcg ttatgaggag ataagtcatg atttttggac atatcgcgca
    17761 gcccaatccg tgccgtttgc ccgccgccat tgaaaaggcg ctcgattttc tgcgcgctac
    17821 caacttcaac gtcctggagc cgggcgttgt cgaaatcgac ggcaagaata tttatgcgca
    17881 aattatcgat ttaaccacac gcgaagcggt ggaaaaccgt ccggaagtgc atcgtcggta
    17941 tatcgatatt cagtttctgg cgtggggtca agagaaaatc ggcattgcta ttgatacagg
    18001 aaataataaa gtcagcgaat cactattaga gcagcgcgat attatttttt atcatgtcag
    18061 tgaacatgaa tcatttatag aaatgatacc gggcagctac gctattttct tcccgcagga
    18121 tgttcaccga ccaggttgta ttctgcaaac aggcctctga gatacgaaaa attgtggtta
    18181 aagtcgcatt aacagcgctg aattaatcaa ataatcgtct acgccagaac gactgggtaa
    18241 tgttattgct ctaactatat taataattta ggttgctgca catgcaactt gaattatgac
    18301 agacatagcc tcaaggaata gctatgaaaa aaatacttga agtaatactg gcgattaatc
    18361 tcgccgtact ttcctgtatt gtgtttataa acattatttt aagatatggt tttcagacaa
    18421 gcattttatc tgtagatgaa ttgtcacgtt atttatttgt ctggttaaca tttattggcg
    18481 cgattgtggc ttttatggat aacgcccacg ttcaggtcac ctttctggtg gagaaactct
    18541 cccccgcatg gcaacgacga gtcgctctgg ttacccattc cttaatctta tttatttgtg
    18601 gcgcactggc ctggggcgca acactaaaaa caattcagga ctggagcgat tattcaccga
    18661 tcctcggttt gcccatcggc ctgatgtatg ccgcttgtct gcccaccagc ctcgttatcg
    18721 cattctttga actgcgtcat ttatatcaac tcatcacgcg tagcaattct ctaacgtcgc
    18781 caccgcaagg agcttaatca tggctgtgct gatttttctg ggctgtctgt tgggtggtat
    18841 tgctatcggt ttgcctattg cctgggcact gttgttgtgc ggggcggcgt taatgttctg
    18901 gctggacatg tttgatgtcc agatcatggc acaaacgctg gtgaacggag ccgatagctt
    18961 ctccctgctg gcgattccgt tctttgtgct ggcgggtgaa atcatgaatg cgggcggctt
    19021 gtcaaaacgc attgttgacc tgccgatgaa actggtgggg cataaaccag gcgggctggg
    19081 ctacgtcggc gtgctggcag ctatgattat ggcgagcctt tctggttctg ccgttgcgga
    19141 taccgccgcc gttgccgctc tactggtgcc gatgatgcgc agcgccaact acccggtcaa
    19201 ccgggcggcg gggctgattg cctctggcgg cattatcgcg ccaattattc ccccttccat
    19261 tccgtttatt atcttcggcg tttccagcgg attatccatc agtaagctgt ttatggcggg
    19321 cattgcccct gacatgatga tgggcgcaac gctgatgctt acctggtggt ggcaggccag
    19381 tcgccttaat ttaccccgcc agcaaaaagc aacgatgcag gaaatctggc actcttttgt
    19441 ctccggtatc tgggctctgt ttcttccagt catcattatt gg
//
LOCUS       DAJTNN010000070        18896 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTNN010000070 DAJTNN010000000
VERSION     DAJTNN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.070"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(21..2468)
                     /gene="glgP"
                     /locus_tag="OO150_002134"
     CDS             complement(21..2468)
                     /gene="glgP"
                     /locus_tag="OO150_002134"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5516278.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDERQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2487..3920)
                     /gene="glgA"
                     /locus_tag="OO150_002135"
     CDS             complement(2487..3920)
                     /gene="glgA"
                     /locus_tag="OO150_002135"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5516279.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRASLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3920..5215)
                     /gene="glgC"
                     /locus_tag="OO150_002136"
     CDS             complement(3920..5215)
                     /gene="glgC"
                     /locus_tag="OO150_002136"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5516280.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5233..7206)
                     /gene="glgX"
                     /locus_tag="OO150_002137"
     CDS             complement(5233..7206)
                     /gene="glgX"
                     /locus_tag="OO150_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5516281.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7203..9389)
                     /gene="glgB"
                     /locus_tag="OO150_002138"
     CDS             complement(7203..9389)
                     /gene="glgB"
                     /locus_tag="OO150_002138"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5516282.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9662..10765)
                     /gene="asd"
                     /locus_tag="OO150_002139"
     CDS             complement(9662..10765)
                     /gene="asd"
                     /locus_tag="OO150_002139"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5516283.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10957..11550
                     /gene="yhgN"
                     /locus_tag="OO150_002140"
     CDS             10957..11550
                     /gene="yhgN"
                     /locus_tag="OO150_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5516284.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11607..12947)
                     /gene="gntU"
                     /locus_tag="OO150_002141"
     CDS             complement(11607..12947)
                     /gene="gntU"
                     /locus_tag="OO150_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5516285.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGPLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12951..13478)
                     /gene="gntK"
                     /locus_tag="OO150_002142"
     CDS             complement(12951..13478)
                     /gene="gntK"
                     /locus_tag="OO150_002142"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5516286.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMLRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13617..14612)
                     /gene="gntR"
                     /locus_tag="OO150_002143"
     CDS             complement(13617..14612)
                     /gene="gntR"
                     /locus_tag="OO150_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5516287.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14836..15531)
                     /gene="yhhW"
                     /locus_tag="OO150_002144"
     CDS             complement(14836..15531)
                     /gene="yhhW"
                     /locus_tag="OO150_002144"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5516288.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15654..16691)
                     /locus_tag="OO150_002145"
     CDS             complement(15654..16691)
                     /locus_tag="OO150_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5516289.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFISDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLSVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17023..17511
                     /gene="yhhY"
                     /locus_tag="OO150_002146"
     CDS             17023..17511
                     /gene="yhhY"
                     /locus_tag="OO150_002146"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5516290.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRSSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17748..>18896
                     /locus_tag="OO150_002147"
     CDS             17748..>18896
                     /locus_tag="OO150_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HCR5516291.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLSKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPP"
BASE COUNT         4825 a         5007 c         4600 g         4464 t
ORIGIN      
        1 cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat atgatcggcg
       61 tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt cagcatcgct
      121 tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg acaatcgaca
      181 tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag cgaatcaacc
      241 agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat ttgcgtcagc
      301 acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc
      361 agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc accgacatga
      421 tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc gttaagcgca
      481 aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg ttcagacaga
      541 tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat gaacacgact
      601 ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc attgatcaaa
      661 tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa aatattcacg
      721 cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt aatcacatgc
      781 aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac atcgaacaac
      841 gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag acgctttttg
      901 ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca gtgttgttgc
      961 agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc gtccagcacg
     1021 gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc gttggtgacg
     1081 ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg caccatcaga
     1141 ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc cagccaggcc
     1201 atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag cagatcggta
     1261 tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc aaagatgatc
     1321 tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc cagcgcctcg
     1381 ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa cgcgtcgtcc
     1441 cagctaaatt ggcgctcatc gatcagcaga cgcatcatct caggaatcga cagtaccgga
     1501 tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt tttatgcaac
     1561 tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccagaaagta ttcctgacgc
     1621 aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg agatacgttc
     1681 tcggagtggt ttttatcttc cactgccgcg aagtagtcac cttggttgaa tttaccgagg
     1741 ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc gtcggtgtcg
     1801 taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc aatccagcgc
     1861 gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg cgtgttgtgg
     1921 cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc tttctggcta
     1981 ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta accgcgcccc
     2041 ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc caggccaccg
     2101 ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa ccccatcgct
     2161 tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt ggagagcgta
     2221 cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg cgacaactgg
     2281 gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa taacgttgcg
     2341 ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa catcagcttg
     2401 taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga
     2461 gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc acggtacgac
     2521 ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac aaaccgccac
     2581 agtgaagcac gagaccacag tacaaaagca cgtcgaatag cccgtaacag cgaccaggca
     2641 ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc aagagaacag
     2701 tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt accgtacttc
     2761 aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag aatgacgtcc
     2821 gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac gcccacctgg
     2881 ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg
     2941 agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag caccaggtcg
     3001 agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt atcgtcaacc
     3061 ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc caacgtatcg
     3121 cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt ctcgtccacg
     3181 ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag accttccata
     3241 ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac cgccgtaatg
     3301 tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa ttccagccca
     3361 tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc ataaaacatg
     3421 ccttgatagg ccaagttgtg cacagtaaac accgacttcg ccggacgccc gcgcgccgcc
     3481 agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc aggacgccag
     3541 aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc aaaacgcaat
     3601 acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg acgatcatag
     3661 agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa caacagcgtg
     3721 atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac gccacggcga
     3781 atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg
     3841 ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa catctctgaa
     3901 catacatgta aaacttgcat tatcgctcct gtttatgccc taacttccgt agcatttcgc
     3961 gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct tcctctgcgt
     4021 tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg cagcggcgca
     4081 gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca atgttgcaga
     4141 atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa ccggagatca
     4201 cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag cgatcctgca
     4261 cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg cgatcgtaca
     4321 tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa gctttcagcg
     4381 tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga
     4441 acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt
     4501 cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga tagtcggcgt
     4561 caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc attgacggtg
     4621 ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc gccataacgc
     4681 caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt
     4741 tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg tcgcccgcca
     4801 ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg gtgaccgcat
     4861 ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc agcagatcga
     4921 caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag
     4981 tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg atgcagttag
     5041 acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt tttgctcgct
     5101 tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg gcaacagatt
     5161 tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta accatgacta
     5221 actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct gccagacagc
     5281 cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact ccccagcagg
     5341 taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc gatccgagag
     5401 cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag gttgagcata
     5461 tcgatttagc caacggacat tgccatcgcc ttcttcccac cagcgattct ccaccaaagc
     5521 gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac cactgcttgc
     5581 ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt tattgccatg
     5641 ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg agagcaacaa
     5701 cgttgttaac aaggcgtgaa tactgtcgcg ccgccgttca accaggtcaa gagaaccgcc
     5761 taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc gattttcttc
     5821 tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc
     5881 atgcgcagtg acgagattaa tcgcggcact cggcagacga ccattacgtt taaaaacatc
     5941 gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat gtagccagaa
     6001 acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg ggaaatttcc
     6061 cacctgataa ccaccagggg cgatatccca cggttcagca attaacttca cctgcgagag
     6121 caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg
     6181 gcccatgact gccgccagat caaagcggaa accatcgacg tgacaggttt ctacccaata
     6241 acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga gcgtgttgcc
     6301 gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc tacggttatc
     6361 gatcccacgc agtgagaata acgggccgtc gaggtccagt tccgcactat ggttgagcac
     6421 gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat cgcgaaactc
     6481 atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca tcgccaccgg
     6541 gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac tggcaaactg
     6601 cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt tgatcatcac
     6661 cggatgcccg agggctttat aagtgccacg gatctcgacc ggaatctccg ggtgcaagta
     6721 cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg tgcgcggcgg
     6781 ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg caatggcggc
     6841 gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat ctttaaattc
     6901 cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa agcgatgccc
     6961 ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc
     7021 cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc gatgttcctg
     7081 gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga aaagtgtgaa
     7141 gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa tggcgagttg
     7201 tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagtgt caggcttagt
     7261 gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc attgcctgca
     7321 ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt gcctggctga
     7381 ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc aacgataatt
     7441 tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt gtcatccacc
     7501 accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt atggtggcgg
     7561 taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt atcgccacct
     7621 tccaacagat gccagtcgag gctggcgtca tggttccact cacggccctg ggcaaattcg
     7681 ttccccatga acagcagttt cttgcccggg aatgcccaca tccagccata gtaggcgcgc
     7741 aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga ttttttaccg
     7801 tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt gtagagaatc
     7861 ccgaaggtga gtttatcgtg atgatactga cgataaaccg ggtcgagctt catgtagtcc
     7921 agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc gcccatatcc
     7981 tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac cgcaccggaa
     8041 acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc aagattctcg
     8101 cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc gcgataaatc
     8161 attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc
     8221 gcgttaccga cgaggaagtt actgacttca cggcgaccat aattgtagat cagcgtattc
     8281 cagtcctgat gatagccttc gcgcggatcg ctgtgttcat acaagttcgt gccatcaaat
     8341 tcggcaagcg caaagtcatc ggtcgggaag tggcctggca cccagtcgag aatcacgttc
     8401 agacccgctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg
     8461 cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa gggatgctcg
     8521 ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg caccagttga
     8581 tcggccagct cgcggtagct caaccagaaa ttgttatcgg tgtggcgacg ccaggagccc
     8641 aggtgaactt cataaataga gattgacgca tcaaactgat tcgctttttt gcgctcttca
     8701 gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg gcgcatttgc
     8761 gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc aatcatctcg
     8821 tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccaaat gccgctctct
     8881 ttacgcagac gcatcgggtg acggcgaccg tcccagtagt tgaattgccc aaccaccgag
     8941 acccgacggg cgtttggagc ccaaacagag aaacgcgtac ctgtgacgcc atccatagta
     9001 tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga taatagccag
     9061 gcatccattt cctggattag cgggccaaaa cggtaaggat catcaatcag gttttgctga
     9121 ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg aatgacaccg
     9181 ctaaagaatc cccgtgagtc aagacactcc agttttgcga gtttgcgccc ggttttcggt
     9241 tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag tcccgcggtg
     9301 gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta
     9361 atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg tgtcacccca
     9421 tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg cgcagcactc
     9481 tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc atgtattcag
     9541 ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga aacatctgcg
     9601 cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta atgaataaag
     9661 attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac agcagctggt
     9721 cacccacggt aaaggctgac aggaactccg gtcccatatt cagcttacgc aggcggccta
     9781 ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata gtgatttccc
     9841 gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct tcgacggtcg
     9901 gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag cgcaatgccc
     9961 cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg atcttgttgg
    10021 tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt ttgtcgatcc
    10081 acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc tcaccgctac
    10141 gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg gtcgcgagtt
    10201 catctgccac atggccatac agatggccca tctgggttaa taactcacgc atatgtcgcg
    10261 caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca agatcattgg
    10321 cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg ccaacaaaag
    10381 tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga
    10441 tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa ccttgccatc
    10501 cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga caggtcacaa
    10561 tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg ccagtggttc
    10621 cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca gggcgaatgg
    10681 cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc ataccgcgcc
    10741 agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg tgctgtataa
    10801 atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa agtcgcaagt
    10861 gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt tgttagcaca
    10921 cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat ttctgcagca
    10981 gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat gtccgtactg
    11041 aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct tattgctctc
    11101 ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct cagcctgcga
    11161 gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat taagatgatt
    11221 ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc atttatcgtg
    11281 ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat gttgttgtct
    11341 catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc ctggggcggc
    11401 acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg
    11461 aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca gatgttcctc
    11521 gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa gaacccggta
    11581 gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat catcccaacg
    11641 atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag cgtttcggct
    11701 tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac gtggctgaca
    11761 acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc ggagtagttc
    11821 agttgttcaa tgaccggcat caccagcccc accgccgtta aacaggcaac ggtggcagac
    11881 ccctgaatga tgcgcactgc agcggccagc acgaagcagg tgatagcaat cggcaggccc
    11941 atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag cacctgtttg
    12001 aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgtag cgcgtgaccg
    12061 caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc ataaatcgct
    12121 accagacaag caaccagaat cgcggtaaat ggatgaccaa taaactcgaa ccattcgtaa
    12181 gcagttgatc cttctggcac aaaacgagcg gcaatggttt tcagccccac cagcaccagc
    12241 ggcaacagga tcagcgacag gctgaatccg aaggacggca ttttgccttc gccgagatgc
    12301 ggttcgctga tgtcgtcagg aatatgcaac tccacgtaac ggctgatgaa attaccccac
    12361 agcggcccgg caataatcat tcccggaatt gccgcacaca gaccaatcag gatcatccag
    12421 ccaaagtcgg cattcatctg cgatgccagc aacatcggcg ctggtccagg caccagaaat
    12481 gccgccgctg ccgccacgcc tgcgaataat gggattacca gcttcaccag gttcgtaccg
    12541 gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa ctatcgccac ttcaaagaac
    12601 agcggtagcg cacagaccaa ccccgcaagg ccgatggcat aatgcgcgcg gctgtgaccg
    12661 aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcatg taagatcttg
    12721 ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggggcc tcccatccct
    12781 ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc agcccccatg
    12841 gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa aaacagcagc
    12901 agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac ttatttgcct
    12961 tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc gatatccacc
    13021 accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg cgtcaccaac
    13081 atttgggttt taaagaaatg gcctttgcgc gctttcaagc gactttcaat cacatcaaaa
    13141 tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa gtcgcgatag
    13201 tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgcag catagcaaac
    13261 gcggcgtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag cggttcgcca
    13321 gacgccattt tttcgatatt gcgccgtgga tggaggaaat caccatcaag aaacgcggca
    13381 tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga tacgcccatc
    13441 aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc acaatacaag
    13501 aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac aatatcagaa
    13561 gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt gtaggcttaa
    13621 atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt cacagattcg
    13681 ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc acgcggcgtc
    13741 agcacgctcg ctaaccgcgg ctccatcacc tggccaatgt catgaccgtg gaaaccggca
    13801 atcgccatat cgtcaggaac cttcaatccc agacgctgac attcaaacgc cgcgccgacc
    13861 gccaggtcat cgttggtaca gaacacgcca tccagctgcg gatattcccg ccgcgcctgg
    13921 cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac gctatacgac
    13981 accagacctg catccagcat cgcctgttcg tatcccttct gtttgatgat agtacgttcg
    14041 tcgagacgcg cgccgagata ggcaatgtgg caatgcccgc gagcaataat ggcggtggtc
    14101 atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca cggcgactgg
    14161 ctgtccatga gttccaccac cgggatcccc gccacttcaa tcatctttaa ggtgcgcggc
    14221 gtgtgggtac gttcggtgag gatcagtccg tcgatattcc atgagagcat ggattcgaga
    14281 cgttcttgct ccatttccgg tttataaccg tagtgagcca gcatggtctg ataaccgtgc
    14341 gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt ggtgagagaa
    14401 ggtaacagga cgccaatcgc ccggctggtg gcgttagaga ggatatcggg cgcacgattg
    14461 ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc gacggaaacc
    14521 tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac acggtcagcc
    14581 acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa gagatagatt
    14641 ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct tgtttacaat
    14701 tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc aaaaccccgg
    14761 aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg caggcttcaa
    14821 agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc tgtcgctatc
    14881 cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg tcgaggcttt
    14941 cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc gttcagcggc
    15001 aatctgatgc accgactgct catctttcag caacgcccag cggtacagtt ccatatcctg
    15061 atgcactttc aatgaaccat ctcgcgcatc cggcgagagc accagctgtt tgccctgtac
    15121 ggcatcgaag cgacgctgtt cataacgcgg cgtaataccg ttttcttcag gcatgatcca
    15181 gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt gacgaatacc
    15241 cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat tgcccatgct
    15301 gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat ctttatgcgg
    15361 gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc gcagcgcgga
    15421 gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc aggagtccag
    15481 ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca tttgtattcg
    15541 ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat gatagagggt
    15601 gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca aacttaattc
    15661 gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat ttccagattg
    15721 gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt ttgatacaac
    15781 gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct gaccgtcacg
    15841 ccctcgtcat tgacatactc cggcacaccg accgaatcat ccgctgcgaa tcccggttcg
    15901 cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta tttaataaac
    15961 gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag atggctggtt
    16021 ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc gtcaggattg
    16081 gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc gaacagagaa
    16141 ataatctggt ccatcgtatg cacaccaagg ccataaaacg cgccatcctg cggcagccca
    16201 ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc aacaatctct
    16261 cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc aaagcgacga
    16321 ttctgatacg gcgtgacgct cagccctttg cttttcgcca acgcaaacag ctccttcgcc
    16381 tgggcaagcg tcggggtgaa cggtttttcg accagcacat ttttcccggc ttccagcgcg
    16441 cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga caacaaccag cttaacatcg
    16501 ggatcgttta gtacttcgtc gagatcgctg atgaaatgga tatgggaata aatgggagcc
    16561 tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct atccttgcgg
    16621 ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat aaaggcgcag
    16681 ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg caaagcggac
    16741 gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa attgagaacg
    16801 aaagatcaaa aaaaagccag cacccggctg gctaagtaat actggaagca atgtgagcaa
    16861 tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata ataatcattc
    16921 tcattcgcgc ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca tttgaaagtc
    16981 aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga gatagtaata
    17041 cgccacgcag aaacacggga ttacgaggcc atcaggcaga ttcacgccca gccggaggtg
    17101 tattgcaaca cactacaggt gcctcatcct tccgatcata tgtggcagga gcgactcgcc
    17161 gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt gggccatctc
    17221 accattgacg tgcaacagcg cccacgcagc agtcatgttg ccgattttgg tatctgtgtc
    17281 gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga tgcgagagat gattgaaatg
    17341 tgcgacaact ggttgcgggt agatcgcatt gaactaaccg tgtttgtcga taacgccccg
    17401 gcaattaagg tctataaaaa atacggcttt gaaattgaag ggactggtaa gaagtacgca
    17461 ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc gggtgaagta agatagtgcc
    17521 ctttttctga gatggaaaaa gggtgtcatt caaaatcgac ataccttcct ttaaggtatt
    17581 tatttaccca atacatatat tgagatttaa ctcataatta attcctaaag tgtaatattt
    17641 tattttttaa tatatacgcc tacaatttcc tggagtaaat aaataacaat taacaagcat
    17701 aatattgcca ttgataaaat agcatgccat aaaaggactt ttcagggatg agtaatattg
    17761 tttacctgac agtaacggga gaacaacaag gaagcatctc cgcaggttgt gggacttctg
    17821 agtctacagg taatcgttgg cagagcgggc atgaggatga aatatttaca ttctcactct
    17881 taaataatat taataatacg gggcttggtt cacagttcca tggtataaca ttttgtaaat
    17941 taattgataa aagcactcca ttatttatta attccattaa caataatgaa caattattta
    18001 tgggatttga cttctatcga ataaatagat ttggtagatt ggaaaagtat tattatatac
    18061 aactaagagg cgctttttta tcggctattc atcaccagat cattgaaaac caactggata
    18121 cagaaacaat aactattagt tatgaattta tcctctgtca acatcttatc gcaaataccg
    18181 agttcagcta tttggcactc cctgaaaatt ataaccgatt gtttttacca aattcaaaaa
    18241 accaaacaaa taatcgtttc aaaacgttaa acagcaaagc tattggcagg ctacttgctg
    18301 ctggtggcgt atacaatggg aacattgaag gattcagaga tactgcggaa aaactgggtg
    18361 gagatgcaat aaaaggctat gatcaaatac taaatgaaaa aacagcgggc atagcgatag
    18421 caacagcatc tattctttta acaaagcgtt ctaatgttga tacatataca gaaataaata
    18481 gttacttagg caaacttaga ggtcaacaaa aacttcttga tggtatagac ataatagaaa
    18541 taatatacat taagagacct tcaaaagact tagctaactt acgaaaggag tttaataaaa
    18601 ctgtaagaaa aaattttctt atcaaacttt caaaaacctc cgaagcatct ggaagattca
    18661 acgccgaaga ccttttaaga atgagaaagg gcaatgttcc tctaaattat aatgttcacc
    18721 ataaactatc tctagatgat ggtggtacta atgatttcga aaatttagta ttaatcgaaa
    18781 acgaaccata tcataaagtt tttactaaca tgcaatcacg aatagctaag ggaatattag
    18841 taggtgaaag caaaatcact ccctgggcca ttccatctgg ctcaatttat cctccc
//
LOCUS       DAJTNN010000071        18765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTNN010000071 DAJTNN010000000
VERSION     DAJTNN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.071"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..312
                     /locus_tag="OO150_002148"
     CDS             <1..312
                     /locus_tag="OO150_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR5516292.1"
                     /translation="PAGELFSDIMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIK
                     TQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKT
                     H"
     gene            412..1662
                     /locus_tag="OO150_002149"
     CDS             412..1662
                     /locus_tag="OO150_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5516293.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPAGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDHLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(1718..2806)
                     /locus_tag="OO150_002150"
     CDS             complement(1718..2806)
                     /locus_tag="OO150_002150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5516294.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGIADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGAGDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(2809..3666)
                     /gene="nfo"
                     /locus_tag="OO150_002151"
     CDS             complement(2809..3666)
                     /gene="nfo"
                     /locus_tag="OO150_002151"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5516295.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(3740..4789)
                     /locus_tag="OO150_002152"
     CDS             complement(3740..4789)
                     /locus_tag="OO150_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5516296.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIIVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            4889..5770
                     /locus_tag="OO150_002153"
     CDS             4889..5770
                     /locus_tag="OO150_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5516297.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANIPR"
     gene            5975..7444
                     /gene="lysP"
                     /locus_tag="OO150_002154"
     CDS             5975..7444
                     /gene="lysP"
                     /locus_tag="OO150_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5516298.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            7737..9717
                     /gene="cirA"
                     /locus_tag="OO150_002155"
                     /pseudo
     CDS             7737..9717
                     /gene="cirA"
                     /locus_tag="OO150_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(9749..10585)
                     /gene="yeiG"
                     /locus_tag="OO150_002156"
     CDS             complement(9749..10585)
                     /gene="yeiG"
                     /locus_tag="OO150_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR5516299.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            10843..11511
                     /gene="folE"
                     /locus_tag="OO150_002157"
     CDS             10843..11511
                     /gene="folE"
                     /locus_tag="OO150_002157"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5516300.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11528..12685
                     /locus_tag="OO150_002158"
     CDS             11528..12685
                     /locus_tag="OO150_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5516301.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            12827..13867
                     /gene="galS"
                     /locus_tag="OO150_002159"
     CDS             12827..13867
                     /gene="galS"
                     /locus_tag="OO150_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5516302.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14147..15145
                     /gene="mglB"
                     /locus_tag="OO150_002160"
     CDS             14147..15145
                     /gene="mglB"
                     /locus_tag="OO150_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5516303.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15206..16726
                     /gene="mglA"
                     /locus_tag="OO150_002161"
     CDS             15206..16726
                     /gene="mglA"
                     /locus_tag="OO150_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5516304.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDKSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            16742..17752
                     /gene="mglC"
                     /locus_tag="OO150_002162"
     CDS             16742..17752
                     /gene="mglC"
                     /locus_tag="OO150_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5516305.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPCWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(17995..>18765)
                     /gene="preA"
                     /locus_tag="OO150_002163"
     CDS             complement(17995..>18765)
                     /gene="preA"
                     /locus_tag="OO150_002163"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR5516306.1"
                     /translation="VEKYCRAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAA
                     INTVKSITNIDLNQKIGMPIVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPIS
                     GIGGIETWEDAAEFLLLGAATLQVTTGIMQYGNRIVEDMASGLSHYLADQGFDSLQEM
                     VGLANNNIVPAEDLDRSYIVYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHC
                     NTEKCVGCLLCGHVCPVGCIELGEVKFKKGEKEHPVTL"
BASE COUNT         4475 a         4616 c         4980 g         4694 t
ORIGIN      
        1 cctgccggag agctgttcag cgacatcatg aacttcactc tcaaaacgca gttcgaaaac
       61 tacggcctgg ctggcggccc ggtgcacgac gccacctgca tcggttatct gattaaccct
      121 gatggcatta aaacccagga gatgtacgtc gaagtggacg tcaacagtgg cccttgctat
      181 gggcgtaccg tctgcgacga gctgggcgtt cttggcaagc ccgctaatac caaagtcggc
      241 atcactattg ataccgactg gttctggggc ttagtcgaag agtgcgtgcg cggctacatc
      301 aaaacccatt aagtgttgat aacaagccgg gtcagtatcg caagatccaa gcccggctat
      361 ttctgatggg cagaaagccg tcgcccaata tgacatgaag agataataac tatggatgtc
      421 atgagaagtg ttctgggaat ggtggtattg ctgacgattg cgtttttgct gtcagtaaac
      481 aagaagaaga tcagcctgcg taccgttggc gcggcgttag tgttacaggt cgtgattggc
      541 gggattatgc tttggttacc ggcagggcgt tgggtcgctg aaaaagtcgc ttttggcgtg
      601 cataaagtga tggcgtacag cgacgcgggt agcgcattta tcttcggttc gctggtcggg
      661 ccgaaaatgg acacgctgtt tgatggcgca ggatttatct ttggtttcag ggtattaccg
      721 gcaattatct tcgtcactgc actggtgagt attctctact acatcggtgt gatggggatt
      781 ttaattcgca ttctcggcgg tatattccag aaagcattaa atatcagcaa gattgagtca
      841 ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa ttccggcgat cgtcaaaccc
      901 tttatcgatc atctgaatcg caatgaatta tttacagcga tttgtagtgg catggcctcg
      961 attgctggtt cgacaatgat tggttacgcc gccctgggcg tacctgtgga atatttgctg
     1021 gcggcatcgt taatggcgat cccaggcggg atcttgtttg ctcgcctgtt aagcccggct
     1081 acggaatctt cgcaggtttc ctttaataac ctctctttca ccgaaacacc gccaaaaagc
     1141 attattgaag ccgctgcgac aggggcaatg accgggctga aaatcgccgc cggtgtagcg
     1201 acagttgtta tggcatttgt cgccatcatt gcgttaatta atggtattat cggcggcgtt
     1261 ggcggctggt ttggttttgc acatgcctcg ctggagtcca ttttaggtta cctgttggcc
     1321 ccattggcgt gggtgatggg ggttgactgg agtgatgcaa atcttgccgg gagtttgatt
     1381 gggcagaagc tggcgatcaa tgaatttgtc gcttatctca atttctcgcc atatctgcaa
     1441 acgggtggca ctctggatgc taaaaccgtg gcgattattt ctttcgcgtt gtgcggtttc
     1501 gctaactttg gttctatcgg ggtggtggtg ggggcgtttt ctgcggttgc gccacaccgt
     1561 gcgccggaaa tcgcccaact tggtttacgc gcgctggcgg cggcgacact ttctaacctg
     1621 atgagtgcta ctattgcagg attctttatt ggtttagcgt agtgcgcaat ttatgagccg
     1681 gataagatgc ggtcaagcgt cgaatccggc aacattgtta caccattggc actaatgaaa
     1741 gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc gctggcgcgc gaaattgccg
     1801 cgcaggctac cgcaaaacgg gcgctgtcgc ggaaactgta tccttcaaga aagctatata
     1861 ccaggcccgc cataaaacca tcgccagcac caaaactgtc tactgtcgta tgcgctggcg
     1921 cggtcagcaa aaattgttct ccatcctttt cgctgcaata aactgactca tcgggcaaat
     1981 aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt cactgcggca ttacggtcag
     2041 catcgctggt gatcgcctgt ccccataaaa tttccagctc cggtaaagtg ggtttcaggg
     2101 tgtgaatatg cgccagccag tgtttgattt tgcccgcttt gaattctgaa acggtatcga
     2161 caaacaccgg gatttcatca gcgagggtaa agacccattc cagcgcctcg gctgtcaggt
     2221 tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc gcgcgacccg ttcagtagtt
     2281 gcggtgtcaa ctgttccagc agatgggtat cgttaatcgc cagcacggtt tgatcgtctc
     2341 gattggcaat tgccagatac gtcgatgtgc tttgaccatg caaacgaacg cagccggaga
     2401 cattcacgcc ggcacggcgc gtttcttcca ggagcatttc gccataaaag tcatcgccaa
     2461 tcactgaaag caaatggacg tcacggccta acagcgccag attgtgggcg atgttgcgtc
     2521 ccacgccgcc cgccgagcaa tgaattgtac cgggatgaga agccgcttgc gggtaacgga
     2581 tatccgcgat cccgcgaata tccatattga ttgtccccac cactacgcag tattcctgct
     2641 cggtgagaat gtaacctttg cctttaatcc gccctttgcg cattaaatcc ataatatgcg
     2701 ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat ttcgttctgc tgaatcagcg
     2761 ggttacgccg taaaattgca aggatctcct tttcccggtt attcatcttc aggctaccgc
     2821 tttttcagtt tgttgcgctt tcagccaggc gatctcttct gcccagatat ccgggttgat
     2881 ggtttcaagg atcagcggaa tgccgtcgaa acggtcgtcc tgcatgatcc agcggaacgc
     2941 atcatgaccg atattgcctt caccgaggct atgatggcgg tcaacgcggc tgccaaaggt
     3001 gcttttcgca tcgttaaggt gcatcccgcg cagatactta aagccaacaa tacgggcaaa
     3061 atccgcgaac gttttctcgc attcggctgg agtacgcaaa tcatacccgg cagcgaaagc
     3121 atggcaggta tcaatgcaga cgccgacacg ggatttatct tccacgccgt cgataatcgc
     3181 cgcgagatgt tcgaatttaa accctaagtt actgccctga ccggcggtgt tttcaatcac
     3241 cgctgttaca ccttgagttt tatccagcgc aatgttgatg gattcggcaa tacgcgcaag
     3301 gcaatcctct tctgaaatct gcatcagatg gctgccaggg tggaagttga gcaaagaaag
     3361 ccccagttgt tcgcaacgct gcatttcatc tataaaggca tcgcgcgatt tttccagagc
     3421 ttcagtgacc ggatgtccga ggttaatcag atagctgtcg tggggaagaa tttgcgccga
     3481 tgtgtagtga tatttttcac aggctgcttt gaattcatcg atggtttgcg tcgtgagcgg
     3541 tgcggcacgc cactgacgtt ggtttttggt gaacaaggca aacgcggttg cgtcgatttc
     3601 ggcggcgcga attgcggcat ttgccagacc gccagcagca ctaacgtgcg ctccaatgta
     3661 tttcatgcga ggactcctgt taaacccgct ggaggaaaac ggtaatgata gcgggttaac
     3721 aggagcaaga tgtagtggtt tatgcgatga cgctttgaat cacatagtta atcgcaccac
     3781 caccaacaat cagccaggca aacagtacca gtgccatcag cagaggtttc gccccggctt
     3841 ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc catcgccatt gccagcagga
     3901 aggtatccag cgttaccagc atgttcacca cgctctgcgg taacaggtgg aacgagttaa
     3961 agatggcaac gatgatgaac aagatggcaa accacggaat agtgattttg cttttctcgc
     4021 cgctgttcgc cccagacagc tgtttaacac gcgccgccag caggatgagg aacggagcca
     4081 gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt ttccgcatcc gggctgatgg
     4141 catgacctgc cgccaccacc tgcgccactt cgtgcacagt agaaccaatg tagataccga
     4201 aagtttccgg actaaaccat tgagacatca gcggatatat cgccgggtag aggaaaatcg
     4261 cgacggtccc gaagataaca acggttgcaa cagccacggt tactttactg gcttccgctt
     4321 tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca gatactgcta ccggcaccga
     4381 tcaaccagct ggtgtgctta tccagaccaa acactttctg ccccaggaag caagccagca
     4441 ggaaggtact ggacagcgtc aacacgtcaa tgatgatccc actgataccg acatcggcaa
     4501 tttgcgagaa cgtcagacgg aagccataaa gaatgatacc cagacgtaat aaatattgct
     4561 tggcaaacag cacaccaccg tcacagcttt tccagatgtg cggatagatg gtgttgccta
     4621 aaaccatccc caacaagatt gcgagggtga gggcactaaa cccggcaccc gcaaccgcgg
     4681 gaatggaacc accccacagg gcgaccccgg tgataactgc actcagggct aaccccggaa
     4741 taaaatgcca cagtgtacga tgttgtttct gtaaggtgat attcgtcata accctctcct
     4801 tataaccggg cataaggtta cgactaactg gtttaaaaat aaaattgatt atatatttat
     4861 aattaatctt tataagtggt aagcgactat gcacatcacc ctccggcagt tggaagtttt
     4921 tgcagaagta ttgaaaagtg gatcaaccac ccaggcgtcg gtgatgctgg cgttgtcgca
     4981 atcagcagtg agcgcagcct tgaccgacct ggaagggcag cttggcgtgc aactgtttga
     5041 tcgcgtgggg aaaagactgg ttgttaatga acacgggcgg ctgctctatc cgcgtgcgtt
     5101 ggcattgctt gaacaggcgg ttgaaatcga acaactgttt cgcgaagaca acggcgcgat
     5161 tcgtatctat gccagtagta ccatcggtaa ctacattctg cctgcagtta tcgcccgtta
     5221 tcgccatgat tatccgcagt tgccgattga acttagcgtt gggaacagcc aggacgtgat
     5281 gcaagcggtg ctggatttcc gcgttgatat tggctttatt gaaggaccgt gccacagcac
     5341 tgaaatcatt tctgaaccgt ggctggaaga cgagctggtg gttttcgccg cgccgacttc
     5401 gccgttggct cgtggtccgg tcaccttaga acagctggcc gctgcgccgt ggatcctgcg
     5461 tgaacgcggt tccggcacgc gggagattgt cgattatcta ttgctgtcac atttaccgaa
     5521 gtttgagatg gcgatggaat taggtaactc cgaggcaatc aaacatgcgg tgcgtcatgg
     5581 gttgggaatt agttgcctgt cgcgacgggt gattgaagat caattgcagg caggcacatt
     5641 aagtgaagtt gcggtccctc tgccgcgcct gatgcgtacg ttgtggcgta tacatcatcg
     5701 gcaaaaacac ctttccaacg cgctacggcg ctttctggac tattgcgatc ccgcgaatat
     5761 accgcgttaa gttgctgtac aagaacatgc tggtgctgtg tcgatttcgt gacgcagcgc
     5821 cctcagcatg cattcgccag aaaagagatt ggctgcttta cttataatcc cagggcgatc
     5881 atgaaggtgt cttataaccg tgtatttctg ccggaaggat tgccaatcgt ctgctacaat
     5941 cgcgcctcat ttttaagatg gatagcattt ttgtatggtt tccgaaacta aaaccacaga
     6001 agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg acgatgattg ccattggcgg
     6061 ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg atttctcagg caggtccggg
     6121 cggggcattg ctctcgtata tgctgattgg cctgatggtt tacttcctga tgaccagtct
     6181 cggtgaactg gctgcatata tgccggtttc cggttcgttt gccacttacg gtcagaacta
     6241 tgttgaagaa ggctttggct tcgcgctggg ctggaactac tggtacaact gggcggtgac
     6301 tatcgccgtt gacctggttg cagctcagct ggtcatgagc tggtggttcc cggatacacc
     6361 gggctggatc tggagtgcgt tgttcctcgg cgttatcttc ctgctgaact acatctcagt
     6421 tcgtggcttt ggtgaagcgg aatactggtt ctcactgatc aaagtcacga cagttattgt
     6481 ctttatcatc gttggcgtgc tgatgattat cggtatcttc aaaggcgcgc agcctgcggg
     6541 ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt ggttttgcgg cgatgatcgg
     6601 cgtagctatg attgtcggct tctctttcca gggaaccgag ctgatcggta ttgctgcagg
     6661 cgagtccgaa gatccggcga aaaacattcc acgcgcggta cgtcaggtgt tctggcgaat
     6721 cctgttgttc tatgtgttcg cgatcctgat tatcagcctg attattccgt acaccgatcc
     6781 gagcctgctg cgtaacgatg ttaaagacat cagcgttagt ccgttcaccc tggtgttcca
     6841 gcacgcgggt ctgctctctg cggcggcggt gatgaacgca gttattctga cggcggtgct
     6901 gtcagccggt aactccggta tgtatgcgtc tactcgtatg ctgtacaccc tggcgtgtga
     6961 cggtaaagcg ccgcgcattt tcgctaaact gtcgcgtggt ggcgtgccgc gtaatgcgct
     7021 gtatgcgacg acggtgattg ccggtctgtg cttcctgacc tccatgtttg gcaaccagac
     7081 ggtatacctg tggctgctga acacctccgg gatgacgggt tttatcgcct ggctggggat
     7141 tgccattagc cactatcgct tccgtcgcgg ttacgtattg cagggacacg acattaacga
     7201 tctgccgtac cgttcaggtt tcttcccact ggggccgatc ttcgcattca ttctgtgtct
     7261 gattatcact ttgggccaga actacgaagc gttcctgaaa gataccattg actggggcgg
     7321 cgtagcggca acgtatattg gtatcccgct gttcctgatt atttggttcg gctacaagct
     7381 gattaaagga actcacttcg tacgctacag cgaaatgaag ttcccgcaga acgataagaa
     7441 ataagtttcc tcccttcctt gctaagccct ctcaaccgag agggcttttt caattccatt
     7501 tccctgacaa atcatgcgga tataaaattt aacatttgga ttgataattg ttatcgtttg
     7561 cattatcgtt acgccgcaat caaaaaaggc tgacaaatca gaggctgttc cggctttctg
     7621 ggatgatcac ctgcatacaa ataagtccac cgcgatgctg ccgtacgcaa ggggacgtga
     7681 agaaggtgtg agcgataacc cattttattt tcgtagttac ctcattgaga tatggaatgt
     7741 ttaggttgaa ccctttcgta cgggtcaggc tgtgtttgtc cgctatttct tgtgcatggc
     7801 ctgtgttagc ggtcgatgat gatggcgaaa cgatggttgt cactgcatct tccgttgaac
     7861 aaaacctcaa agatgcaccc gccagtatca gcgtcattac ccaggaagac ctgcagcgaa
     7921 aaccggtaca gaatctgaag gatgtcctca aagaagtgcc tggcgtacaa ctgacgaacg
     7981 aaggggataa ccgtaagggc gttagtattc gtggtctgga cagcagctac accctgattc
     8041 tcgtcgacgg taaacgcgtt aactcccgca atgccgtctt ccgccacaat gatttcgatc
     8101 tgaactggat cccggtcgat tccatcgaac gtattgaagt ggttcgtggc ccgatgtcgt
     8161 cgctatacgg ctccgatgcg ctcggcggtg tagtgaatat catcaccaaa aaaatcggtc
     8221 agaaatggtc gggtaccgtt accgtcgata ccaccattca ggaacatcgc gatcgcggtg
     8281 atacctataa cggtcagttc tttaccagtg gaccattaat tgatggtgtg ctgggaatga
     8341 aagcttacgg cagcctggca aaacgtgaaa aggatgaccc gcaaaactca acgaccaccg
     8401 ataccggaga aatgccgcgt attgaaggat tctccagccg cgacggcaat gtcgaatttg
     8461 cctggacacc gaatcaaaat cacgatttta ctgccggata cggtttcgac cgtcaggaac
     8521 tcgctggaca aaaaccgcct ggaacgccag aactactccg tcagccataa tgggcgttgg
     8581 gattacggca ccagcgaact gaaatactac ggtgagaaag tcgagaacaa aaaccctggc
     8641 aacagcagcc cgataacttc cgaaagcaat acggtcgacg gcaaatacac gttgccgctg
     8701 acggcgatta atcagtttct cacggttggc ggtgaatggc gtcacgacaa acttagcgat
     8761 gcggtgaacc tgaccggggg aaccagctcc aaaacgtctg ccagccagta cgcgctgttt
     8821 gtggaagatg aatggcggat cttcgagccg ctggcgctga cgaccggcgt gcgtatggac
     8881 gatcacgaaa cctacggtga acactggagt ccgcgtgcct acctggttta taacgccacc
     8941 gacaccgtaa cggtgaaagg gggctgggcg acggcattta aagcaccttc tctgttgcaa
     9001 cttagccctg actggacgag caattcctgc cgtggcgcat gtaagattgt gggtagcccg
     9061 gatctgaaac cagaaaccag cgaaagttgg gagctggggc tttactacat gggtgaagaa
     9121 ggctggctgg aaggggggga atccagcgtt accgttttcc gtaacgatgt gaaagatcgt
     9181 atcagcatca gccgtacgtc tgacgtcaac gctgcaccgg gctaccaaaa ctttgttggt
     9241 tttgagacgg gcgctaacgg acggcgtata ccggtattta gctactacaa cgttaacaaa
     9301 gctcgtattc agggcgtgga aaccgaactg aaaattccgt tcaacgatga atggaaactg
     9361 tcgatcaact acacctacaa cgatggtcgt gatgtcagca acggcgaaaa caaaccgcta
     9421 tccgatctgc cgttccatac tgctaacggt acgctggact ggaaaccgct ggcgctggaa
     9481 gactggtcat tctatgtttc tggtcactat accgggcaga aacgcgccga cagcgcgacg
     9541 gctaaaacac cgggcggtta taccatctgg aataccggcg cggcctggca ggtgactaaa
     9601 gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag tcgtgacgac
     9661 tacagctata acgaagacgg acgtcgttac tttatggcag tggattatcg cttctgatga
     9721 gaagatgccc ggcgaaccgg gcggactttc acttcagtaa atactgcgca tggaagcgca
     9781 ggtgatcctc tataaaagag gcgatgaagt agtaactgtg atcatatccc ggctgaatac
     9841 gcagcgtcat cggccacgct ttctggcgcg cggcttctgc cagtaccgca ggttgcaact
     9901 ggtcggcaag aaactgatca ttatcgccct gatcgataag cgtcgggatc gcatcctgcg
     9961 cgttactggc atacatcagc gcgcaactgt cccattccag ccatgcattt ttgtcctcac
    10021 ctaaatagct gctaaacgct ttgattcccc acgggacgct gcacggattc acaattggcg
    10081 caaaggccga aacgctggtg tatttacccg gatttttcag cgccataatc agcgcaccgt
    10141 gaccacccat tgagtgaccg ctaatggcgc agcggtcgct gacattaaat tgcgactgaa
    10201 ccagcgccgg taattcatcg cgcagataat catacatccg gtaatgcgtc gcccacggcg
    10261 gttgcgtggc attaagataa aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg
    10321 caaccttttc gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc agttctgccg
    10381 ccacgcgctg ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa
    10441 gccagtacag cactggtggc ggggtgtgat cacgaggggg tgggagaaag atactgaacg
    10501 tcatcgggca gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac
    10561 agcggtgctc ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc
    10621 atcataccgt ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg
    10681 ttttaacatc tgctgatgaa aggcagcggc aattacaata attatcgctg tgaatactgg
    10741 attatgtgcg ccgcctcacg cacaataatc aggctgtaaa tcagcttaat aactttgccc
    10801 ccacgcaggg cggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa
    10861 gaagcggccc tggttcatga agcgttagtt gcgcgaggac tggaaacacc gctgcgcccg
    10921 cccgtgcatg aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa
    10981 atcatgcagc tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc
    11041 atcgctaaaa tgtatgtcga tgaaattttc tccggtctgg attacgccaa cttcccgaaa
    11101 atcaccctca ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact
    11161 ctgaccagta cctgtgaaca ccattttgtt accatcgatg gcaaagcgac ggtggcttat
    11221 atcccgaaag attcggtgat cggtctgtca aaaattaacc gtatcgtgca gttctttgcc
    11281 cagcgtccgc aggtgcagga acgtctgacg cagcaaattc ttattgcgct acaaacgctg
    11341 ctgggcacca ataacgtggc tgtctccatt gatgcggtgc attactgcgt gaaagcgcgt
    11401 ggcatccgcg atgcaaccag tgccacaaca acgacctctc ttggtggatt gttcaaatcc
    11461 agtcagaata cgcgccacga gtttctgcgc gctgtgcgtc atcacaactg attaaaaggc
    11521 aggaaccatg gagcgcaacg taacgctcga ttttgttcgc ggcgtcgcca ttctggggat
    11581 cctgctatta aacatcagcg cctttgggct accaaaggcg gcttatctca atcccgcctg
    11641 gtacggcgct attacgccgc aggatgcatg gacctgggca tttctcgatc tcatcggcca
    11701 ggtgaaattc ctcacgcttt ttgcgctgct gtttggtgcg ggcctgcaaa tgttgctgcc
    11761 ccgtggcaga cgctggatcc agtcgcggtt aacgctgtta gtcttgctgg gttttattca
    11821 cggtttattg ttctgggacg gcgatattct gctggcttac gggctggtgg gcttaatctg
    11881 ctggcggctg gtgcgcgatg cgccatcggt aaaaagcctg tttaatacag gcgtcatgct
    11941 ttatctggtg gggcttggcg ttttgctgtt attggggctg atttccgaca gccagaccag
    12001 ccgcgcctgg acgccggatg catcggctat tttgtatgaa aaatactgga agcttcacgg
    12061 cggcgttgaa gcgatcagta atcgtgccga tggtgttggc aacagtttac tggcactggg
    12121 cgcacagtat ggctggcaac tggcagggat gatgctcatt ggtgcggcgc tgatgcgcag
    12181 cggctggctg aaagggcagt tcagcttacg tcactatcgt cgtactggtt ttgtgctggt
    12241 ggcgattggg gtgaccatta accttcctgc catcgccctg caatggcagc tggactgggc
    12301 gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc cgtttcaggc
    12361 gattggctat gcgtcgctgt tttatggctt ctggccgcaa ttgagccgct ttaagctggt
    12421 gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac tatctattgc aaacgctgat
    12481 ttgtaccatg cttttttacc acctcggttt gtttatgcat tttgaccgcc tggagctgct
    12541 ggcgtttgtt attccggtat ggctggcgaa tatcctcttc tctgttatct ggctgcgtta
    12601 cttccgccag gggccggtgg aatggctctg gcgtcagtta actttgcgtg ctgccggaac
    12661 ggcaatatct aaaacatcaa gataacgatc tggatcacat tcgttaacaa aacggctgta
    12721 accgtttcca ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg acaaaatagc
    12781 cgccagcaag cagtcattta ctgcaatctc ataacaggta gtgaatatga tcaccattcg
    12841 tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt tcccgggtgc tcaataacag
    12901 cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg agctggatta
    12961 tcggccaaac gccaatgccc aggcgctggc aactcaggtt agcgacacca ttggcgtggt
    13021 ggtgatggac gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg atctagtcgc
    13081 tcagcagcat cagaaatacg tgctaatcgg caatagctat catgaagcgg aaaaagagcg
    13141 tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc actcaaaagc
    13201 attgagtgac gatgaactgg cgcaatttat ggataacatt cccggtatgg tgttaatcaa
    13261 ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg gataatctca gcggtgcccg
    13321 aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt attggttatc tttcttccag
    13381 tcacggcatt gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca
    13441 ggatattatt ccgccggaaa gctggattgg cactggtacg ccggacatgc cgggcggtga
    13501 ggcggcgatg gttgaactgc tggggcgcaa tctacaactt accgctatat ttgcttataa
    13561 cgacaatatg gccgctggcg cactgacagc attaaaagat aatggcattg cgattccgtt
    13621 acatctctca atcatcggtt tcgatgatat tcccatcgcc cgttacaccg acccgcaatt
    13681 aacgaccgtg cgttatccca ttgcttcaat ggctaaatta gccaccgaac tggccttgca
    13741 gggggcagca ggcaatattg atcctcgtgc cagccactgt tttatgccga cgttagtgcg
    13801 tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc actaactcaa caaatcaggc
    13861 gatgtaaccg ctttcaatct gtgagtgatt tcacagaatc ttaacaatgt gatagctatg
    13921 attgcaccgt tttaacgttg taacccgtat gtaacagtga ataatcactt tcaccgaggt
    13981 aatagcgtca taacaacaat taaagccgtt ttctggagcg ttaccgggca tggaagaacg
    14041 aattttaaaa agtgagcttc ggcgttcagt aacacttcat taactctact gccctgccga
    14101 gcatttatct caagcactac cctgcataag aaaaaccgga gataccatga ataagaaggt
    14161 gttaaccctg tctgctgtga tggccagcat gttattcggt gccgctgcac acgctgctga
    14221 tactcgcatt ggtgtaacaa tctataagta cgacgataac tttatgtctg tagtgcgcaa
    14281 ggctattgag caagatgcga aagccgcgcc agatgttcag ctgctgatga atgattctca
    14341 gaatgaccag tccaagcaga acgatcagat cgacgtattg ctggcgaaag gggtgaaggc
    14401 actggcaatc aacctggttg acccggcagc tgcgggtacg gtgattgaga aagcgcgtgg
    14461 gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc tggatagcta
    14521 cgacaaagcc tactacgttg gcactgactc caaagagtcc ggcattattc agggcgattt
    14581 gattgctaaa cactgggcgg cgaatcaggg ttgggatctg aacaaagacg gtcagattca
    14641 gtccgtactg ctgaaaggtg aaccgggcca tccggatgca gaagcacgta ccacttacgt
    14701 gattaaagaa ttgaacgata aaggcatcaa aactgaacag ttacagttag ataccgcaat
    14761 gtgggacacc gctcaggcga aagataagat ggacgcctgg ctgtctggcc cgaacgccaa
    14821 caaaatcgaa gtggttatcg ccaacaacga tgcgatggca atgggcgcgg tagaagcact
    14881 gaaagcacac aacaagtcca gcattccggt gtttggcgtc gatgcgctgc cagaagcgct
    14941 ggcgctggtg aaatccggtg cactggcggg cacagtactg aacgatgcta acaaccaggc
    15001 gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc aaaggtgcgg ctgatggcac
    15061 caactggaaa atcgacaaca aagtggtccg cgtaccttat gttggcgtag ataaagacaa
    15121 cctggctgaa ttcagcaaga aataagactg attgggcgca gtcgtttact gcgcccttat
    15181 tgtatggcca acaaggtaaa attatatggt cagctcaacg actccgtctt ccggggaata
    15241 cttgttggaa atgagcggta tcaacaagtc ctttcctggt gttaaggcac ttgataacgt
    15301 taatttaaaa gtccggccac attctatcca tgcattaatg ggggaaaacg gtgcaggaaa
    15361 atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca ccattttatt
    15421 ccagggtaaa gagatcgatt tccattccgc caaagaggca ctggaaaatg gtatttcgat
    15481 ggtacaccag gagttaaacc tggtattaca acgttcggtg atggataaca tgtggctggg
    15541 gcgatatccc accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg aaaccaaagc
    15601 gatttttgat gaactggata ttgatatcga tccgcgtgcg cgcgtcggca cattatccgt
    15661 ttcgcaaatg cagatgatcg aaatcgccaa agcgttttcc tataacgcga aaattgtgat
    15721 tatggatgaa ccgacttctt cgttaaccga aaaagaggtc aatcatctgt tcactattat
    15781 tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa tggaagaaat
    15841 cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg ccaccgaacc
    15901 gctggcagga ctgacgatgg acaagatcat cgccatgatg gttgggcgtt ctcttaatca
    15961 gcgttttcct gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc gtaacctaac
    16021 gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag gggagatcct
    16081 cggtattgcc gggctggtgg gggcgaaacg taccgatatt gttgagacgt tatttggtat
    16141 tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa cagatcaata accataatgc
    16201 caacgaagcc ataaaccacg gatttgcact ggtaactgag gagcgccgct caacgggaat
    16261 ttatgcctat ctggatattg gttttaactc gttaatttcc aatattcgca actacaaaaa
    16321 taaagttggt ttactggata aatcgcggat gaaaagcgat acccagtggg tgattgattc
    16381 gatgcgggta aaaacaccgg ggcatcggac gcaaattggt tcgctctcag gtggtaatca
    16441 gcaaaaggtg attattgggc gctggctact aacgcaacca gaaatattaa tgctcgatga
    16501 accgacgcgc ggtattgatg tcggggcgaa gtttgagatc tatcagttaa ttgccgaact
    16561 ggcgaagaaa ggcaaaggga ttattattat ctcctctgaa atgcctgagt tgttagggat
    16621 aacagaccgt attctggtca tgagcaatgg tctcgtttcc ggaattgtcg acacaaaaac
    16681 aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt aggggcttcc
    16741 catgagtgcg ttaaataaga aaagttttct tacttacctg aaagagggcg gtatttacgt
    16801 cgttctttta gttttgctgg cgattattat tttccaggac ccaacatttt taagtctgtt
    16861 gaacttaagt aatattctca cccagtcatc ggtgcgtatt attatcgcgc tcggtgtggc
    16921 agggttaatt gtcacccagg ggaccgacct ttctgcgggg cgtcaagtag ggctggcagc
    16981 agtggtggca gcgacattat tgcagtccat ggataacgcc aataaagtgt tcccggaaat
    17041 ggcgacgatg ccgattgcgc tggttattct gattgtctgt gccattggtg cggtgatcgg
    17101 tttgatcaac ggtctgatta tcgcttatct caacgttacg ccgttcatta ccacgctcgg
    17161 cacgatgatc atcgtctatg gcatcaactc gctctattac gactttgtcg gggcgtcgcc
    17221 aatttctggt tttgacagtg gcttctctac ctttgctcag ggctttgtcg cgctggggag
    17281 tttccgtctc tcttacatca ccttctacgc gttgattgcg gtggcgttcg tctgggtgtt
    17341 gtggaacaaa acccgcttcg gtaagaacat ttttgccatt ggcggtaacc cggaagcggc
    17401 aaaagtatct ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt tgtctggcgt
    17461 gttctatgcc tttggcggga tgttagaagc cggacgtatc ggctctgcca ccaacaacct
    17521 cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggtg tatcgttcag
    17581 cggcggtgtg gggacggtga ttggcgtggt gaccggggta attattttta ccgtcatcaa
    17641 ctatggcctg acgtatatcg gcgtaaaccc atgctggcag tacatcatca aaggggcgat
    17701 tattatcttc gccgtagcgc tggattcact gaaatacgcg cgtaagaaat gatgtgaatg
    17761 tggtccgcat tcggtcataa acgcacataa catcatgatc tgtgtggttt tgtaggccgg
    17821 ataaggcgtt cacgccgcat ccggcaacag gagccaaatg ccggatgcaa cgctggcgcg
    17881 ttttatccgg cctacaataa gctcaacata ctgatttgta tggttttgta ggccggataa
    17941 ggcgttcaag ccgcatccgg cgacaggaac caaatgccgg atgtgacgcg ggttctacaa
    18001 cgttaccggg tgttctttct cgcctttctt aaacttcact tccccgagct caatacaacc
    18061 caccgggcag acgtgaccac aaagcagaca acccacacat ttctcggtat tacaatgcgg
    18121 tgtgcgggtt ttctcgctcc attccatcgc ctggtgaccg ccgtcgtaac aggaaatata
    18181 acagcgtcca cagccaacac atttgtcaag attgatacgg ggatagacaa tataactgcg
    18241 gtctaaatct tccgccggga caatattgtt attcgccaga cctaccatct cctgcaacga
    18301 atcaaatccc tgatcggcga gataatgcga taacccgctc gccatatctt ctacgatgcg
    18361 attgccgtac tgcataatgc cggtggtgac ctgtaacgtt gctgcgccga gcaataaaaa
    18421 ctccgccgca tcctcccagg tttcaatgcc gccgataccg ctgattggga aatcgcgcag
    18481 ttctggatgg gtgcgcattt gctgaatgaa gcgcagggcg atcggtttta ccgctttacc
    18541 ggaatatccg gaaatactcg atttaccgtt aacgatcggc ataccgattt tctgattaag
    18601 atcgatattg gtgatggatt taacggtgtt aattgccgca atgccatctg cgccgccgcg
    18661 cttcgccgcc agcgccactt cgcacatatc accgatattc ggcgtcatct tcgccagcat
    18721 tggcagcgtg gaaccgcgtt tcaccgcccg acaatatttt tctac
//
LOCUS       DAJTNN010000072        18745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTNN010000072 DAJTNN010000000
VERSION     DAJTNN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.072"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..504)
                     /locus_tag="OO150_002164"
     CDS             complement(<1..504)
                     /locus_tag="OO150_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR5516307.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEI"
     gene            971..2203
                     /gene="mdtM"
                     /locus_tag="OO150_002165"
     CDS             971..2203
                     /gene="mdtM"
                     /locus_tag="OO150_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCR5516308.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2244..3524
                     /locus_tag="OO150_002166"
     CDS             2244..3524
                     /locus_tag="OO150_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCR5516309.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            3640..4792
                     /locus_tag="OO150_002167"
                     /pseudo
     CDS             3640..4792
                     /locus_tag="OO150_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            4802..5570
                     /locus_tag="OO150_002168"
                     /pseudo
     CDS             4802..5570
                     /locus_tag="OO150_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5567..5824
                     /locus_tag="OO150_002169"
     CDS             5567..5824
                     /locus_tag="OO150_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCR5516310.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            5778..6749
                     /locus_tag="OO150_002170"
     CDS             5778..6749
                     /locus_tag="OO150_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCR5516311.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNPIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            6817..7989
                     /locus_tag="OO150_002171"
     CDS             6817..7989
                     /locus_tag="OO150_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5516312.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALARGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIALGNEYVLAGL
                     HFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAILYG
                     LAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPCLT
                     ANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLLGF
                     VTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS"
     gene            complement(8002..8556)
                     /gene="kptA"
                     /locus_tag="OO150_002172"
     CDS             complement(8002..8556)
                     /gene="kptA"
                     /locus_tag="OO150_002172"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5516313.1"
                     /translation="MAKHNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            8806..9489
                     /locus_tag="OO150_002173"
     CDS             8806..9489
                     /locus_tag="OO150_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516314.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9486..9947
                     /locus_tag="OO150_002174"
     CDS             9486..9947
                     /locus_tag="OO150_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5516315.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            9960..11132
                     /gene="iadA"
                     /locus_tag="OO150_002175"
     CDS             9960..11132
                     /gene="iadA"
                     /locus_tag="OO150_002175"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5516316.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11197..12108
                     /gene="hypT"
                     /locus_tag="OO150_002176"
                     /gene_synonym="qseD"
     CDS             11197..12108
                     /gene="hypT"
                     /locus_tag="OO150_002176"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5516317.1"
                     /translation="MDDCCAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12101..12493)
                     /gene="iraD"
                     /locus_tag="OO150_002177"
     CDS             complement(12101..12493)
                     /gene="iraD"
                     /locus_tag="OO150_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5516318.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12490..12573)
                     /gene="idlP"
                     /locus_tag="OO150_002178"
     CDS             complement(12490..12573)
                     /gene="idlP"
                     /locus_tag="OO150_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5516319.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            13165..13995
                     /locus_tag="OO150_002179"
     CDS             13165..13995
                     /locus_tag="OO150_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5516320.1"
                     /translation="MSIPLSNALQSQIITDNHFLHHPKVDNKLTREYEYTRMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNIGKQSWPFNYATDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGYNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(14136..14909)
                     /gene="uxuR"
                     /locus_tag="OO150_002180"
     CDS             complement(14136..14909)
                     /gene="uxuR"
                     /locus_tag="OO150_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5516321.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15124..16584)
                     /locus_tag="OO150_002181"
     CDS             complement(15124..16584)
                     /locus_tag="OO150_002181"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5516322.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLKAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(16665..17849)
                     /gene="uxuA"
                     /locus_tag="OO150_002182"
     CDS             complement(16665..17849)
                     /gene="uxuA"
                     /locus_tag="OO150_002182"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5516323.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18189..18393
                     /locus_tag="OO150_002183"
                     /pseudo
     CDS             18189..18393
                     /locus_tag="OO150_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18636..>18745)
                     /locus_tag="OO150_002184"
     CDS             complement(18636..>18745)
                     /locus_tag="OO150_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5516324.1"
                     /translation="TSAVSLGLTANYARTGGQVTAGNVQSIIGVTFVYQ"
BASE COUNT         4381 a         4750 c         4732 g         4882 t
ORIGIN      
        1 aatctcgtcg tctggcacga cagtgacatc caccagcggg aacgctgcgc tataaagttt
       61 ccgtgcggta gtcggggcgg caaattcgtc cagccagcac agggaccagg ggtaaggact
      121 acggctaccg tgataaaaca gcatcgggat aaccagcggt agcggccggc gtttatcatg
      181 ctcgatatgg cgctgcatca ccgccatgga atatcgcatc aggcgaaatg ccatatgaat
      241 atcctcgcgg ctctgatgtt caatcactac gtaaatataa ccatcacctt cacgggtctt
      301 taccgaccac agaatatcgg agtgtagcgc ccgcaatttt tcatcgacaa agctggcgga
      361 ttccagtttt aagctgtcga gatcgcacag ttcacgtaaa tctttgggta agtggatctc
      421 cataaaatcc cgcgcggtgt cagggtgagt gagaaaggat ttaaataatg cgtcatgtgg
      481 cgtgctggtc gtgaagtttg tcatggcgat ccgtcacctg tagataagat ggcccgactt
      541 tagcgactaa caaaaccatt ctcacccggc aatttctcgt tccccgaggt gctttccagg
      601 cgtctgcaaa cgatacattt ttccggaaac aggctcgcgt tttacatttc ctgtacccgg
      661 cagatattcc cctttccacc gcgctctttt gattaaccgc aaattttaat tacacttaag
      721 gtgtatattt tctatgcaac ccatcaatta aagaggtgta atgtgctgat gactatttgt
      781 aatcgttata cattctgacc tggagtcaga aggtatttct ctgtctgtgt gttcacaggc
      841 agtgtggttg attacatgaa attcagtaca tttgcagtct cgttgccctt ctcgcctgcc
      901 tttcgtcatt accgacggta ttgaatttcg ttttccccgt tggggttctc cgaacaagga
      961 gttgtttgtt atgccacgtt tttttgcccg ccatgccgcc acgctgtttt tcccgatggc
     1021 gttgattttg tatgactttg ccgcgtacct gtcgacggat ctgatccagc cggggatcat
     1081 taatgtggtc cgtgatttta atgccgatgt cagtctggcc ccggcagccg tcagtctcta
     1141 tctcgcgggc ggtatggcgt tacagtggct gctggggccg ctttccgaca ggattggccg
     1201 caggccggtg ctgattaccg gggcgctaat atttaccctt gcctgcgctg cgacaatgtt
     1261 cacaacgtct atgacacagt ttcttatcgc gcgtgcaatt cagggcacca gtatctgttt
     1321 tattgccacc gttggttatg tcacggtgca ggaggcgttc ggccagacaa aagggatcaa
     1381 gttgatggcg attatcacct ccatcgtact gattgcgccg attattggcc cgctttccgg
     1441 cgcagctctg atgcactttg tgcactggaa agtccttttt gccatcattg cagttatggg
     1501 ttttatctca tttgttggct tactgctggc gatgccagag acggtgaagc gcggcgcggt
     1561 tccatttagc gccaaaagcg tctttcgcga ttttcgtaat gtcttttgca atcggctgtt
     1621 cctctttggc gcagcaacca tctctttaag ctatatccca atgatgagtt gggtggctgt
     1681 ctcgccggtg atcctgatcg acgcgggcag cttaacaact tcgcagttcg cctggacaca
     1741 ggttccggtg ttcggcgcgg tgattgtcgc gaatgccatc gtggcgcgtt tcgttaaaga
     1801 tccgaccgaa ccgcgcttta tctggcgtgc tgtacccatt caactggtcg gcctcgcgct
     1861 gttgattatc ggcaatctgc tgtcaccgca cgtctggctg tggtcggtgc tgggcaccag
     1921 tttgtatgct ttcgggattg gtttgatctt cccgacctta ttccgcttta cgcttttttc
     1981 caataactta ccgaaaggga ccgtctccgc atcgctgaat atggtgatcc tgatggtgat
     2041 gtctgtctcg gtcgaaatcg gccgctggct gtggtttaac ggcggtcgct tgccgtttca
     2101 tctgttagcc gtcgtggcgg gcgttatcgt cgttttcacc ctggcaggat tgcttaatcg
     2161 cgtgcgccaa catcaggctg ccgagctggc ggaggagcgg tgatttttgc gcgatcctgc
     2221 cgtcaggctc tattcttaac gttatgaata aactcattga actcagacgc gccaaaatgt
     2281 tggcgctctc tttactgctt atcgccgctg ctacctttgt cgttacgctg tttttgccgc
     2341 ccaatttttg ggtgagcggc gtgaaggcga ttgctgaagc ggcgatggtc ggcgcgctgg
     2401 cggactggtt tgcggtggtg gcgctgtttc gccgcgtgcc gattccgatc atttctcgtc
     2461 atacggcgat tatcccgcgt aataaagacc ggattggcga aaatctcggt cagttcgtgc
     2521 aggaaaaatt tctcgatacc cagtcgctgg tggcattgat tcgacgtcac gaaccggcgt
     2581 tgttgattgg caactggttt agtcagccgg agaacgcccg ccgcgttggt cagcatctgt
     2641 tgcagatcat gagcggtttt cttgaactga ccgatgatgc gcgtattcag cgcctgctta
     2701 agcgcgcggt ccatcgggcg attgataagg tcgatctttc cggaaccagt gcgttgatgc
     2761 tggagagtat gaccaaaaac gatcgtcatc aggtgctgct cgatacgctg atcgcacagt
     2821 tgatcgccct tctccagcgc gataaatcgc gcaagtttat tgcccagcaa attgttcgct
     2881 ggctggagag cgagcatccg ctgaaagcca aaattttgcc tactgaatgg ctgggcgaac
     2941 atagcgcgga gttggtttct gacgcggtga attctttgct tgatgatatc agccgcgatc
     3001 gtgcgcatca gatccgtcat gcgtttgatc gcgccacttt tgccctgatc gacaagctga
     3061 aaaacgatcc agaaatggca gcgcgagccg atgccgtaaa aagttatctg aaagaagatg
     3121 aagcttttaa ccgctatctc agtgaattgt ggggggattt acgggaatgg ctgaaagcgg
     3181 atatcaacag tgaagattct cgtgtgaaag aacgtatcgc gcgggcgggt caatggtttg
     3241 gcgaaacgtt aattgccgat gatgccttgc gggcatcgtt aaatggtcac ctggaacaag
     3301 ccgcgcaccg cgtcgcgcct gagttttccg cattcctgac gcgccacatc agcgatacag
     3361 taaaaagctg ggatgctcgg gatatgtcgc ggcaaatcga gttaaatatc ggcaaagatc
     3421 tgcagtttat ccgtgtcaac ggtacgctgg ttggcggttg tattgggcta attttatatt
     3481 tgttgtcgca gctcccggcc ttgttccccc tcagcaattt ttagaaatcc atgaaaaact
     3541 aagtggctaa ccgcgagaga gatcaaataa accacactaa aagaatggta ataatgtggc
     3601 ctcttttcat tatctcccgt ggtacgggga aggaaaatca tgtcacttat caccgatcta
     3661 cccgccattt tcgatcagtt ctctgaagct cgccagaaag gctttctcac cgtcatggat
     3721 ctcaaggagc gcggcattcc gctggttggc acttactgca cctttatgcc gcaagagatc
     3781 ccgatggcag ccggtgcggt tgtggtttcg ctctgttcta cctctgatga aaccattgaa
     3841 gaagcggaga aagatctgcc gcgcaacctc tgcccgctga ttaaaagcag ctacggcttc
     3901 ggcaaaaccg ataaatgccc ctacttctac ttttcggatc tggtggtcgg tgaaaccacc
     3961 tgcgacggca aaaagaaaat gtatgaatac atggcggagt ttaagcccgt tcatgtgatg
     4021 cagttgccga acagcgttaa ggacgatgcc tcgcgtgcgt tatggaaagc cgagatgctg
     4081 cgcttgcaaa aaacggtaga agaacgtttt gggcacgaga ttagcgaaga tgctctgcgc
     4141 gatgccattg cgctgaaaaa ccgcgaacgt cgcgcactgg ccaattttta tcatcttggg
     4201 cagttaaatc ctccggcgct tagcggcagc gacattctga aagtggtcta cggcgcaacc
     4261 ttccggttcg ataaagaggc gttgatcaat gaactggatg caatgaccgc ccgcgttcgt
     4321 cagcagtggg aagaaggcca gcgactggac ccgcgtccac gcattttaat caccggctgc
     4381 ccgattggcg gcgcagcaga aaaagtggtg cgggcgattg aagagaatgg cggctgggtt
     4441 gtcggttatg aaaactgcac tggggcgaaa gcgaccgagc taatgcgtgg cggaaacggg
     4501 cgatgtctac gacgcgctgg cggataaata tctggcgatt ggctgctcct gtgtttcgcc
     4561 gaacgatcag cgcctgcaaa tgctcagcca gatggtggaa gaatatcagg tcgatggcgt
     4621 agttgatgtg attttgcagg cgtgccatac ctacgcggtg gaatcgctgg cgattaaacg
     4681 tcatgtgcgt cagcagcaca acattcctta tatcgctatt gaaacagact actccacctc
     4741 agatgtcggg cagctcagta cccgtgtcgc ggcctttatt gagatgctgt aaggagtggc
     4801 agtgacatat tcgattggca ttgattctgg ttctaccgcc accaaaggga tcttactggc
     4861 agacggcgtg attacgcgcc gtttcctcgt tccaacaccc tttcgtccgg caacagcaat
     4921 tactgaagcc tgggaaactc tgcgcgaagg gttagagaca acgccgtttc tgacgctcac
     4981 cggctatgga cggcaactgg tggattttgc cgataaacaa gtgacggaaa tctcctgtca
     5041 cgggctgggc gcacggtttc ttgcgccagc aacgctgcgc ggtaatcgac atcggtggtc
     5101 aggacagcaa agtgattcag cttgatgatg acggtaacct gtgcgatttc ctgatgaatg
     5161 acaaatgcgc ggcgggcacc gggcgtttcc tggaagtgat ctcgcgcacg cttggcacca
     5221 gcgtcgagca actcgacagc attaccgaaa atgtcacgcc gcatgccatc acgagtatgt
     5281 gcacagtgtt tgccgaatca gaagtgatca gcctgcgctc agcgggcgtc gcgccagaag
     5341 cgattctcgc cggagtgatt aacgcgatgg cgcggaggag tgccaatttc attgctcgtc
     5401 tctcctgtga agcgccgatt ctgtttactg gtggcgttag tcattgccag acgtttgccc
     5461 ggatgctgga atctcacctg ggaatgccgg taaataccca tcctgatgcg caatttgctg
     5521 gcgcaattgg cgcggcggta attggtcaac gagtgaggac acgccgatga aagagttttt
     5581 atttttattt cactccacag tcggcgtcat acaaactcgc aaagcgttgc aggcagcggg
     5641 catgactttt cgcgtcagcg atattccccg tgatttacgc ggcggctgcg ggttatgcat
     5701 ttggctgacc tgtccccccg gcgaagaaat acaatgggtg atccctgggc tgactgagtc
     5761 aatttattgc cagcaggatg gtgtctggcg ctgcattgca cattaccggg tttctccgcg
     5821 ttaaggattc attaaggtcg ctacgtatac ttctcgatat cttattgatt tgccttaaag
     5881 ggattaccgt gacaaaaagt atttcgttaa gtaaacgcat ctccgttatt gtgattcttt
     5941 ttgccattgt ggcagtgtgt actttcttcg tgcaatcttg cgcccgtaaa agcaatcatg
     6001 cagcatcatt tcagaattac cacgccacca ttgacggcaa agaaattgca ggcataacga
     6061 ataatatctc ttcgctgacc tggtcggcac aaagcaatac tctttttagc actatcaaca
     6121 aacctgccgc cattgttgaa atgaccacca atggtgattt tatccgcaca attcctctgg
     6181 attttgttaa ggatctcgag acgatcgaat acattggcga taatcagttt gtcattagcg
     6241 atgagcgcga ttacgccatt tatgtgattt cactgacccc aaactcggaa gtaaaaatcc
     6301 tcaaaaaaat aaaaatcccg ctgcaagact ccccaaccaa ttgcggtttt gaagggttag
     6361 cttattctcg ccaggatcac actttctggt tttttaaaga gaaaaatccg attgaggtct
     6421 acaaagttaa cgggctgtta agtagcaatg aattacacat cagcaaagac gaggcactac
     6481 aacggcaatt tactttagat gatgtgtccg gcgcagaatt taaccagcaa aaaaatacgc
     6541 ttctggtgct gtctcatgaa tctcgcgcac tccaggaagt aaccctggtt ggcgaggtta
     6601 tcggtgaaat atcactaacc aaaggtagtc gaggtttgtc gcataacatc aaacaggccg
     6661 aagggattgc gatggatgcc tctggtaata tttatatcgt tagcgaacca aatcgttttt
     6721 atcgctttac ccctaaatca tcgcattaac aggctggcat atttcacctt tttgttatta
     6781 acttgttaac aacaaaaatg acatacaagg ttaaacatgc cttcgtcctc gcatcccgta
     6841 gaacgctttt ctttcagcac cgcgcttttc gggatgctgg ttctgaccct aggtatggga
     6901 ttaggtcgct tcctttatac gcctatgttg cccgtcatta tggcggaagg ggcgttttca
     6961 tttagccagc tctcgtggat tgccagcggc aactatgcgg ggtatctggc tggcagtctg
     7021 ctattttcgt ttggcgcatt tcaccagccg tcgcgcctac gcccatttct gttggtttct
     7081 gccctggcga gagggttgtt gatcctcgca atggcatggt tgccgccatt tattctggtg
     7141 ttattgattc gcttcctggc gggtgtcgcc agcgccggta tgctgatttt tggttcgacg
     7201 ctgattatgc agcacacgcg ccatcctttt gtgctggcag ctttgttttc tggcgttggc
     7261 attgcactgg gcaacgaata tgttctggca ggcctgcatt ttgccttctc ctcgcaaacg
     7321 ttatggcaag gcgccggggc gctttctggc atgatgctga tcgcacttac gcttttaatg
     7381 ccctcgaaaa aacacgccat cgcaccaatg ccattggcaa aaacggagca acagataatg
     7441 agctggtggt tactggctat tctgtacggc cttgcgggat ttggttatat catcgtcgcg
     7501 acttacctgc cgctcatggc gaaagatgca ggctcaccat tgttaaccgc ccatctctgg
     7561 acgttagtcg gcttatcaat tgtgccaggc tgctttggct ggctatgggc agcaaaacgt
     7621 tggggggctt tgccttgcct gacggcgaat ttgctggtgc aggctatcag tgtgctgctt
     7681 actctcgcca gcgactctcc tctcttgctt atcattagta gtatcggttt tggcggtacc
     7741 tttatgggca cgacatctct ggtgatgact atcgcccgcc aactgagcgt tccgggaaat
     7801 ctcaatcttt taggctttgt gacgctcatt tacggcattg gacaaatcct cggcccggcg
     7861 ctgaccagta tgctcggcaa tggaacatcg gcgcttgccg gcgccactct ctgcggcgcg
     7921 gcggcgttgt ttattgctgc gcttatcagc acagtacaat tatttaagct gcaagtggtc
     7981 acttcatgag gagtgtttcc cttaccactc aagaaattcg accgcaaccg ttgatgtcag
     8041 ccagactccg ttttccgctt gccagaaagg aatgcctcgt ttcgccattt cctgtgcttt
     8101 gacggttaag acaaccggcg atccgtgtct tgctcccact ttgcgtgccg ttgcttcatc
     8161 agcagagaga tgcacgtaat gacgatcccc tgcaatcagc ccctgttttt ttatctcgtc
     8221 aaggaaacgg cttgccgtac cgtggtagag aaactgtggc ggtgtttttt cggcaaagga
     8281 aatagcgacc tgcgaagtcg aatgcccctg aaccgcgcgg atgcacctac catcactgga
     8341 ataactaaaa cgctttttat cgctggttgc gaccacggta tccagcaatg cacgggttaa
     8401 tcgcttgccc gctttttggg cacaaagtat gagcttatcg atatccgccc atccttcacg
     8461 gtccagtacg ataccaatcg cctccggttt gtgtcggagg acaaaactta aaaacttgct
     8521 cgtatccgcc agctcttttt cgttgtgctt tgccatctcg ttttcgtccc tggattttct
     8581 gtttttattt accaatgtca ttgtcactgg gtttatgaat tttctgcaac tcaatcctgc
     8641 gcagaacctt ttccacacag cgtaatgttt cccctatgca aaaaacgcat agccagaaac
     8701 atacttgcat tttccccata cccacatggc tgataaggtt tttcagttcc aggaattttc
     8761 ctggctcaag caacacacaa acacaaaaaa acaatgggga ttgttatgac acaacaaggg
     8821 gatgctgtag ccggggagct ggcgacagaa aaagtgggca ttaaagggta tctggctttt
     8881 tttctcacca taatcttttt ttccggtgtt ttttccggaa cagacagctg gtggcgcgtt
     8941 tttgattttt ccgttctgaa cggatcgttt gggcaactgc ctggtgcgaa cggagccacc
     9001 acctcatttc gcggcgcggg tggtgcgggg gctaaagatg gctttctctt tgcgctggag
     9061 ctggcaccgt cagtcattct ctctctgggg attatttcaa ttaccgatgg tcttggtgga
     9121 ttacgcgccg cgcagcagtt aatgacacct gtgctaaaac cgttgttggg cattccaggg
     9181 atctgctcgc tggcgctgat cgctaacttg caaaacactg atgccgccgc cggtatgacc
     9241 aaagagctgg cgcaggaagg tgaaattacc gaacgcgata aagtgatttt cgccgcatat
     9301 caaaccagcg gtagcgccat catcaccaac tacttctctt ccggcgtcgc ggtgttcgcc
     9361 tttctgggca cgtccgtaat cgtaccttta gccgtcatcc tggtgtttaa atttgtcgga
     9421 gccaacattt tgcgcgtctg gcttaacttc gaagaacgtc gcaacccaac gcaaggagcg
     9481 caagcatgac aactcaggtc cgcaaaaacg tcatggatat gtttatcgat ggcgcgcgcc
     9541 gtggctttac tatcgccacg accaacctgc tgccaaacgt ggtgatggcg tttgtcatca
     9601 ttcaggcgct gaaaattacc ggcctgctcg actgggtggg gcatatctgt gaacccgtaa
     9661 tggcgctgtg gggattaccc ggtgaagcgg caaccgtcct tttagccgca ttaatgagta
     9721 tggggggcgc agtcggtgtt gccgccagtc tggcaaccgc tggcgcatta accggtcacg
     9781 atgtcaccgt tttactgccc gcgatgtatt taatgggcaa cccggtgcaa aatgtcggtc
     9841 gctgcctggg gacagccgaa gtgaatgcca aatattaccc gcatatcatt acggtctgcg
     9901 tgattaacgc attgctgtcg atctgggtta tgcagcttat tgtttaacaa ggagttacca
     9961 tgattgatta taccgcagcc ggttttaccc tgctgcaggg agcgcatttg tatgcgccgg
    10021 aagatcgggg aatttgcgat gtcctcgtcg ctaacggcaa aattatcgcc gttgccagca
    10081 atatcccttc tgacattgta ccgaactgca cggttgtcga tctcagtggg cagatcctct
    10141 gcccaggttt tattgatcaa cacgtccatt tgattggcgg tggcggcgaa gcaggtccca
    10201 cgacgcgcac gccggaagtg gcgctaagtc gcctgacgga agcgggcgtc acgtcagtgg
    10261 ttggtctgct gggcaccgac tctatctctc gccacccgga atccctgctc gccaagaccc
    10321 gtgcgctcaa tgaagaaggc atcagcgcct ggatgctgac cggcgcttat catgtccctt
    10381 cccgcactat tacgggttcc gtggaaaaag acgtggcgat tatcgatcgt gtgattggcg
    10441 tgaaatgcgc catctctgat caccgttctg ccgcaccgga cgtttatcac ctggctaata
    10501 tggcggcaga atcccgcgtt ggcggtttgc tcggcagtaa acctggcgtc accgtgttcc
    10561 acatgggcga cagtaaaaag gcgttacagc ctatttatga cctgctggaa aactgcgatg
    10621 tgccgatcag caagctgctg ccgacccacg ttaaccgcaa cgtaccgttg tttgagcagg
    10681 cgctggagtt cgcgcgcaaa ggcggcacca tcgatatcac cagcagcatt gacgaaccgg
    10741 tcgcccctgc cgaaggtatt gcccgcgccg ttcaggcggg tattccgctg gcacgcgtca
    10801 ccctcagctc cgacggcaac ggtagccagc cgttcttcga tgacgaaggg aatttaaccc
    10861 atatcggtgt tgcaggtttt gaaacgttgc tggaaaccgt gcaggtgctg gtcaaagact
    10921 atgatttcag tatcagcgat gccctgcgcc cgctcaccag tagcgtagcc ggtttcctta
    10981 acctgagcgg gaaaggcgaa attctgcctg gcaatgacgc agacttacta gtcatgacgc
    11041 cagaattgcg cattgagcag gtatatgctc gcggcaaact gatggtcaaa gacggcaaag
    11101 cctgcgtgaa aggaacgttt gaaacggctt aaacggttgt cagccgccct aatggacgta
    11161 gaatgcccgc aagggcggct gacagagtaa aacgtaatgg atgactgttg tgcggttttg
    11221 cataatattg aaaccaaatg gctttatgat tttctgaccc tggaaaaatg ccgcaatttt
    11281 tcccaggcgg cagtcagtcg taacgtctcg caaccggcat tcagccgccg catccgcgcg
    11341 ctggaacagg cgattggcgt tgaattgttt aaccgccagg tgacgccgct gcaactctca
    11401 gaacaaggga aaatcttcca ttcgcagatc cgccatctgt tgcaacagtt agaaagtaac
    11461 cttgcagagc tgcgtggcgg cagcgattac gcgcaacgta aaatcaagat agccgctgca
    11521 cactctcttt ccctcggtct gttaccgtcc attatcagcc agatgccgcc gctctttacc
    11581 tgggcgattg aagctattga tgtcgatgaa gcagtcgata aactgcgtga agggcaaagt
    11641 gactgtattt tttcctttca cgacgaagat ttgctggaag caccgtttga ccacattcgc
    11701 ttatttgaat ctcaattgtt ccccgtttgc gccagtgacg aatacggaga agcaattttt
    11761 aacctcgcgc agccacactt tccgttactg aattacagcc gcaactccta catggggcga
    11821 ttgattaatc gcaccctgac gcgccacagc gagttaagtt tcagcacctt ttttgtctct
    11881 tcgatgagcg agctattaaa gcaggttgcc ctcgacggct gtgggattgc ctggctgccg
    11941 gagtatgcca tccaacaaga aattcgcagc gggcaactcg ttgtgctgaa tcgggacgaa
    12001 ctggtgatcc cgattcaggc ctacgcatac cgtatgaata cccgcatgaa tccagttgcc
    12061 gaacgcttct ggcgtgaact gcgcgagctg gagattgtgc ttagctgaca ttctcaagcg
    12121 tcgcactgcg ccaaccgcca ttatcccaga ccagatcaaa cgttagcggt tcatcgtcgt
    12181 ggtacagcat tatctcaagg ctgatcgcca gagtgtggca gtacgcttct ttcaaattaa
    12241 cctgcaacgc cgcaatccgt ggctcatgaa ataacagtgc agaacgcagc tgacggcaat
    12301 accagctctg gagatgtttg tcgctcgggt tatgcacttg ccactcgggc atgcctgaaa
    12361 caagtaatga cggtaatgcc gaatgaggag aattaaaaag agtgaggaga tcgctgcata
    12421 ccgatttacg cagcaatgac gttttattgc ctgaaatttc aggtaaaaca gcctgaagtg
    12481 attggcgcat cattttgcgc actcctgacg tttagcaaca tatgcggctt gccctgaaca
    12541 ccgggcacca ctgtattgat gctcattttc catagccatt cccttttgtt cattacatcc
    12601 atgtcagtct gagacaggcc tgaatcctgt cagcaaagta taaaacctta cttgcctatg
    12661 aatatctact gacttttcag gaaaaaagtc atgatttgat aaaaatcatc aaactcaaaa
    12721 aaaaaccgct agtatattct gaatattccg aatagttacg tagatgaaaa tacttgtcac
    12781 cgccgctact taattccggt ggaaaattat ttatttccgc ataatcagcc atgtattatt
    12841 tgaaacaatt agtttgtaaa taatataaat tgataacata tcaaatacat caaaaatacc
    12901 attcataggc attattaata tagacatcaa tatagcaaca tcaaattcca attagtaata
    12961 agatatgctt tttaattgca tgaaaataat ttcaaatgag cgttttttga tactttagag
    13021 tgtttcctac cctttaatat caccgtgtag agttgatatc actacaacag aaacatgcat
    13081 gtcatttttt tacttctttt ttactacaga aaaatttatc tatttttcta aacatcggtg
    13141 aaatactcat aaaaggtaac tattatgtct ataccattaa gcaatgccct tcagagtcaa
    13201 attattacag ataatcactt ccttcatcat ccaaaagtcg acaataagtt aacacgtgaa
    13261 tatgaatata cacgcatgga tacggaaaat atctatcttc tgcctcttac tcgtggaaat
    13321 aaccataatt acgatggaaa aagcgttgtc gaaattagaa aacttaatat tggcaaacag
    13381 tcctggccat ttaattatgc gacagataca tgcagagaat tcaatggtat tacaacaaca
    13441 ggacgtatgc tctatcgaaa cttgaaaatc acctcagctt tagacgaaat atatggtggc
    13501 atatgtaaaa aggcgcatgc tgctacagag ttagcagaag gtttacggct aaatttattt
    13561 atgaagagcc ctttcgatcc tgttgaggac tatacagttc atgaaataac attaggccct
    13621 ggttacaatg ttcctggtta tgccggaacc acgataggat atatttcaac ccttccagct
    13681 tctcaggcaa aaagatggac caatgagcag ccaagaattg atatctatat tgatcagata
    13741 ataactgtct caggggtggc aaattcttcc ggattcgccc ttgccgcatt acttaatgct
    13801 aatattggaa tgggaaatga cccaataatt ggtatagaag cctatcctgg taccgcggag
    13861 atccatgcaa aaatgggata taacgttatc cccggtgatg aagatgcgcc actcaaacgc
    13921 atgactctgc aaccgtcatc tcttccagaa ctgtttgaat taaaaaatgg tgaatggaat
    13981 tatattggca aataaccact gagaagaatg agtactaaca ttcaggttat atagatattt
    14041 aaacaatacc ctgcaattta caaagtaata ctcattgcga cttcttaatt taagaaagcg
    14101 caaggaacgt ttacccttgc gcttatcata atcagtcagg cgtcgacttt atccagcggc
    14161 catgaatcaa acagatagcc gtcaaaataa atatcgtcaa cgttcgagaa ttccagcaga
    14221 cgttgcttaa cgttttccag atgctgccac attgccagct tcgccgctcg ggcatctttt
    14281 ttgattaacg cggcaaggat ctgtttgtga tcgcccaacc actctttgcg atacaggctg
    14341 tcatccagat ggctgtgcaa ctggatccac attggattgt tttcccgcca ctgccaggac
    14401 tgacggaata actccaccag catgctgtta tgcgttgctt cagcaatggc gagatggaac
    14461 tgcatgtcac cgctttcgct gctacccggc gcgctggaag ccagttcacg ctcttccagt
    14521 tgcaatgcct gacgcatttt gacgatatct tcgcgggtag cctgcagggc ggcaaattcc
    14581 gcgatgttgc tctcaagaag ctgtcgcgcc tgcaacaatt caaaggggcc ggcatcattg
    14641 cagacgttgg catccggact gtctgtgttg tggctgcctg agctatcaag aacatagata
    14701 ccggcacccc ggcgcacttc caccagccct ttgatctcca gcatgatcag cgcttcacgt
    14761 accaccgtcc gcgtgacatc aagcatttct gcaatttcac gctccggcgg cagccgttcg
    14821 ccaggattgt acggcgtctt tatgatcaga tcgcggatca tcgccccgac ttcctggtaa
    14881 ggtctttgcg cagaggtggc agatttcatg gtgttgtgtc cgttaggtgc ggttaatcaa
    14941 atttttgccg gatgcggcgt gaacgcctta tccggcctac aacgggcaat tttacggccc
    15001 ccgttaatct caaactatac cattagcact ggaagttggc acgtacaggt gggcaaaaaa
    15061 acgaaaactg gtcaaccaaa tatcagtttg attgaccagt aagtctgtag ggtaatcagt
    15121 tagttacagc gcagccacac actcgcgcgc accgcgttcg cacagctgct gatatgccgc
    15181 cgtcaccgcg ccaacaaagt cggcattctg cggcagatca tcggcaaaaa taccgctcag
    15241 gcccagcagc gctttcacgc ggtctgcgcc ctgatactgc gcgttaatct tctggaactc
    15301 ggccagcatc gggtcgacca cgtcaatggc attaccctgc tcatccacgc cctgggtgta
    15361 acgcatccag ccagccacgc ccagcgccag gtgacgccag ctgccgccgt tttgcaggtg
    15421 cagacgcacc gggtccagca gacgctgcgg taacttctgg ctgccgtcca tcgcaatctg
    15481 ccaggtacgg tgacgcagag acgggttgct gaaacgctcg atcagcagcg tcgcataggc
    15541 cttcaggtcc gtaccctccg gcatagacag cgttggcgct tgttcctgca tcatcagggc
    15601 aaaggccgct ttgcgataat ccgggttagt catggtgtcg gcaatagttt catagccgcc
    15661 gaggtaaccg aggtatgcca ggaaagagtg gctgccgttc agcatacgca gcttcatcat
    15721 ttcgaacggc acaacgtctg caacgaactg tgcgcccact ttatcccagt ccgggcgacc
    15781 attaacgaag ttatcttcaa tcacccactg acggaacggt tcgcaggcaa tggcgcacgg
    15841 gtcgtaaaca cccagctggt cagcaatttc ctgtaaggtt tctggcgtcg ccgccggaac
    15901 gatgcggtca accatggtgc acgggaaggt gacgttctct tcaatccatg ccgccagctg
    15961 cgggtcacgt gcctgagcca gccccagtac cgcgaccttc gccacatgac cgttttcacg
    16021 cacgttatcg caggacatca ccgtaaatgc tttcagccct ttttcacgac gcagacgcaa
    16081 ggcttcgacg atgtaaccaa tcgcggactt cggcgcagtc gggttttcta gatcgtgctt
    16141 gatcagcggg ttattgagat ccagttgacc gcttgccgca tcagcgcagt agcctttttc
    16201 cgtgaccgtt agagagacaa tcgccgtttg cggacgcgcc atcgcgttga gaataccttc
    16261 gcagccgtcg atttccggat gcagcgcttc tttcatcgaa ccgataattt tcagctcggt
    16321 gctttctgcg cctttttccg ctacggtgta cagcagttgc tgttttttca ggttttcgat
    16381 cagcacgcgg tcgttgcctg gcatcaggtt aacttcgcag atgccccagt cgctgtcggt
    16441 gctttccagc agatgatggg tatacagcgc ctgatgtgcg cggtgaaacg ccccgcaacc
    16501 gagatgcaca atgcgtgatt ccagacgaga atgatcccat gacggacggg caaccggcag
    16561 attgctgtca acaatagtag tcatcgtaaa ctccagaatc cgtcttcaga gagtgacggc
    16621 aaggaagaag gaaccggcac ggcgcgcagc catgccggtg gatattaacg gctaaagaaa
    16681 gcgcgctgga tcgccagttc gacaccgcga acttcggcca ggcctttcag acgaccaatt
    16741 gcggagtaac ctgggttggt tttcttcttc aggtcgtcca gcatctgatg accgtggtcc
    16801 ggacgcatcg ggatcaggtc ttctttgcct tccgctttac gacggtgttc ttcttcaaca
    16861 atcgctttca ccacttcgta catatcaacg tcaccgttca ggtgcgccgc ttcgtggaag
    16921 gttttcgggt tatcttcacg catggtggag cgcagatggg tgaagtaaat acgcggaccg
    16981 aactgcttga tcatatcaac cagatcgttg tcagcacgca cgccgtagga accggtgcac
    17041 atggtgaaac cgttcgccat gctgtttacg gtatcaacca tccactgcat atcttcaatg
    17101 gtggaaacaa tgcgcggcag gccgaggatc gggcgcggcg gatcgtccgg gtgaaccgcc
    17161 atacgcacgc caacttcttc agcaactgga ataatcgctt tcaggaagac ggcaaagttt
    17221 tcgcgcagct tcgctttatc gatatctttg tacagctcca ggtgcttacg gaactggtcg
    17281 agggtatacc cttcttccgc gcccggcaga ccggcaatga tgttacgcgt cagacgcgct
    17341 ttgtcttcat cgctcatagt ggcgaagcgt tcagctgcct gagcaatttc ttcttcggtg
    17401 taatccgctt ccgcgcctgg acgtttcagg atatgcattt cgaatgcagc gaattcgatc
    17461 tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt attcgaggtc agtacgggtc
    17521 cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa tgccgcactg cgccaggttg
    17581 cgcagagtct gctgatagtt agcgatccac tgctcatagt tgccagtgtg ggttttgata
    17641 tcttcgtgaa ttggtacgct ttctacgaca gaccacacca ggcctgcgtc ttcaacgatc
    17701 gccttgcgtt tgaggatctc ttctacggac catacttcgc cgttcgggat atggtgcagc
    17761 gcggtaacca cgccagttgc gcccgcctga cggacatcag ctaaagaaac cagatcgttt
    17821 gggccgtacc agcgccaggt ctgttccatg atttttcctc tttcacatag tgggttccac
    17881 agcaaagaga cgcggaaccc tggagttgtt taggtatccg gatgaacaac cagaccgcag
    17941 ccgtggcgtt gcatcggatg agatggcgta taagttctac cttttggtaa gatatgtcaa
    18001 cccaaatcct gaaattggtt aaccaaatca caagaatttc actctcatgg ttgttgctgc
    18061 aaattgcagc aaaaggtaat cgatggttat tgatcagagg atgtgacatt catcgcaaca
    18121 atggttgacc aatttacata acatgtcgcc caaaataaca acggttcaac caggtaaggt
    18181 aaaacgttat gcatgtgctt aacattctct gggtagtatt cggcattggt ctgatgctgg
    18241 tactgacaac gaccgcaggt tcagacacat tcactgcatt agcaatgcct cttagattat
    18301 tttgacctgt cagtaaaaga cacgttgaaa acctggggtc tgctggaact ggtcaactcc
    18361 gtggttgggc tgattattag caaggtagcg taaaagaaca ccagagcctg ccaatggcag
    18421 gctctgactg atgacaaagt ctaaaaatgt gcccggacag tcccctcgcc cctccgggga
    18481 gagggttagg gtgaggggaa cagaccagca aagtgcgctt tgtttatgcc agatgcgacg
    18541 ctgacgcgtc ttatctggcc tacaaagggc taacgtgcag gtttttagct tcaggtaata
    18601 ttgcgtaact gcattagcaa tgccctgtga tttctttatt gataaacaaa agtcacgcca
    18661 ataatcgatt gcacattccc tgcagtcacc tgccctccgg tacgtgcgta atttgccgtt
    18721 aatcccagac ttaccgccga agttc
//
LOCUS       DAJTNN010000073        18330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTNN010000073 DAJTNN010000000
VERSION     DAJTNN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.073"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..720
                     /locus_tag="OO150_002185"
     CDS             <1..720
                     /locus_tag="OO150_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5516325.1"
                     /translation="MLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRT
                     MWGMMLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMTAIPFLFGAAGMLVNGYV
                     TDWLVKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGT
                     SCRGLIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVT
                     AAGALAYIFLVRQPINDPRKD"
     gene            complement(769..2424)
                     /gene="tsr"
                     /locus_tag="OO150_002186"
     CDS             complement(769..2424)
                     /gene="tsr"
                     /locus_tag="OO150_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5516326.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2543..2989)
                     /gene="hpaR"
                     /locus_tag="OO150_002187"
     CDS             complement(2543..2989)
                     /gene="hpaR"
                     /locus_tag="OO150_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5516327.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3261..4550
                     /locus_tag="OO150_002188"
     CDS             3261..4550
                     /locus_tag="OO150_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5516328.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            4547..6013
                     /gene="hpaE"
                     /locus_tag="OO150_002189"
     CDS             4547..6013
                     /gene="hpaE"
                     /locus_tag="OO150_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5516329.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRDHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6015..6866
                     /gene="hpaD"
                     /locus_tag="OO150_002190"
     CDS             6015..6866
                     /gene="hpaD"
                     /locus_tag="OO150_002190"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5516330.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            6876..7256
                     /locus_tag="OO150_002191"
     CDS             6876..7256
                     /locus_tag="OO150_002191"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5516331.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7367..8170
                     /gene="hpaH"
                     /locus_tag="OO150_002192"
     CDS             7367..8170
                     /gene="hpaH"
                     /locus_tag="OO150_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5516332.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8181..8969
                     /gene="hpaI"
                     /locus_tag="OO150_002193"
     CDS             8181..8969
                     /gene="hpaI"
                     /locus_tag="OO150_002193"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5516333.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9144..10520
                     /gene="hpaX"
                     /locus_tag="OO150_002194"
     CDS             9144..10520
                     /gene="hpaX"
                     /locus_tag="OO150_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5516334.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10530..11416
                     /gene="hpaA"
                     /locus_tag="OO150_002195"
                     /pseudo
     CDS             10530..11416
                     /gene="hpaA"
                     /locus_tag="OO150_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11667..13229
                     /gene="hpaB"
                     /locus_tag="OO150_002196"
     CDS             11667..13229
                     /gene="hpaB"
                     /locus_tag="OO150_002196"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR5516335.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSANDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13247..13759
                     /locus_tag="OO150_002197"
     CDS             13247..13759
                     /locus_tag="OO150_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5516336.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14152..16302
                     /gene="btsT"
                     /locus_tag="OO150_002198"
     CDS             14152..16302
                     /gene="btsT"
                     /locus_tag="OO150_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5516337.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16433..16636
                     /locus_tag="OO150_002199"
     CDS             16433..16636
                     /locus_tag="OO150_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5516338.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16647..17603
                     /gene="yjiA"
                     /locus_tag="OO150_002200"
     CDS             16647..17603
                     /gene="yjiA"
                     /locus_tag="OO150_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5516339.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17705..>18330)
                     /locus_tag="OO150_002201"
     CDS             complement(17705..>18330)
                     /locus_tag="OO150_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR5516340.1"
                     /translation="GSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGS
                     LNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSN
                     WKTPAMALTFRANNFFDVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDV
                     VAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWLTKQDVEAHKES"
BASE COUNT         4196 a         5081 c         4884 g         4169 t
ORIGIN      
        1 atgctctatc gcaaccgcga gcacgtagaa ctgactgccg ttgagcaagc ttatctcaat
       61 gcaggtagcg tcaatgcccg ccgagatccg ctcagttttg ccgaatggcg cagcctgttc
      121 cgtaaccgta caatgtgggg aatgatgctc ggattcagtg gcatcaacta cactgcgtgg
      181 ctgtatctgg cctggcttcc tggttacctg caaacagcct ataacctgga tttaaaaagc
      241 acagggttga tgacggctat ccctttcctg tttggggctg ccgggatgct ggtcaacggt
      301 tacgttaccg actggctggt caaaggggga atggctccga ttaaaagccg taagatctgc
      361 attattgccg ggatgttctg ttctgccgcc tttacgctga tagtaccaca agcgacaaca
      421 tccatgacgg cggttctgct gattggcatg gcactgttct gtattcactt tgccggaaca
      481 tcctgccggg gcttgatcca cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt
      541 atccagaact ttgccagctt catctgcgcc tcttttgcgc cgatcattac tggttttatt
      601 gttgatacca cccactcatt ccgtctggca ctaatcatct gcggttgcgt caccgcagcg
      661 ggggcactgg cgtacatctt cctggttcgt cagccgatca acgacccacg taaagattaa
      721 tctccttatg cccgataaca ttttgcttat cgggcatttt catggcgatt aaaatgtttc
      781 ccagttctcc tcgctatctg ccacggccat tttacgcggc gcagctggcg tcacggtttt
      841 taccacagcc gatgtttcac gctgctgttg ctgaatccgg aacactgcca ctgcttcggt
      901 caggcgactg gcctgctctt ccagcgcggc ggcggcagcg gcagactctt ccaccagcgc
      961 ggcgttctgt tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc
     1021 acggctctgc tcatcagaag cagaagcaat ttcgcccata atgtccgtca cgcgggtcac
     1081 ggcgctgaca atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc
     1141 cactttcccc accgagtctt caatcaggct tttaatttca cgagccgcct gggcgctgcg
     1201 ctgggccaga ttacgcactt ctcccgcgac caccgcaaaa ccgcgccctt gctcacccgc
     1261 acgcgcagcc tcaaccgccg cgttcaaagc cagaatattg gtctggaagg caatgccgtc
     1321 aattacgctg ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg
     1381 caccacgtta tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaacgccag
     1441 atggctggcc tggcgcgcat tttcggcgtt ctgcttcacc gttgcggtca gttgctccat
     1501 gctggctgcc gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcgtt
     1561 attgccggtg gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc
     1621 accgacggta cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat
     1681 ctcattagag ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat
     1741 gctgtcaatc aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc
     1801 gaagatgacc gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta
     1861 ggaggcattg ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc
     1921 cacatactgc ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt
     1981 gattttgcct gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta
     2041 attacgtttg atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc
     2101 gtaatccgcc cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc
     2161 agcaacggtt gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg
     2221 gttgagggtg ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tggattgctg
     2281 ctggcgaatg gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaagaa
     2341 cagaccgcct gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt
     2401 cacgatcttg atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg
     2461 gcctggaaag caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg
     2521 ctggttatca gatactaaaa agttattcat tatcgcccgg gatggcttct tgtcgggaat
     2581 tgcccagagc aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa
     2641 actgagcttc aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg
     2701 cctgcccctc tttggttagc gagatataga gcttccgctg atcattgatc ggctttaatc
     2761 gcaacactaa accgtcgcgt tccatccgcg tgagaatccc ggtcaggctt gggcgcaaaa
     2821 tgcaggcgcg atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca
     2881 cgatgcgcca ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca
     2941 tcgccgcttc tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc
     3001 tgaaaaattc aaaatcatta atatagaaac agttaatcgt cacgttaccg ggactgcgct
     3061 gcccctgcaa ttcctgattt tatcaaaatt gatgatattt tattaactta ctgatattta
     3121 atattttatt atctttaaat gtaaatagtt tgttaattag atcacattta catcacttac
     3181 ttttgccaaa ctacgcaatc gggaataaaa gcaatcatta acatattaat gattaagata
     3241 atcgaatccg aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca
     3301 gccaacttga tgcatggcag gaagcgttcc agcaatcccc ctacaaagcc ccgcctaaaa
     3361 ctgcggtctg gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct
     3421 ttccacaggg tgaaaaggta ctgagcggtg cgactgttgc gctgattgtg ggaaaaacgg
     3481 cgacgaaagt acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg
     3541 acgtcagcct gccggaagag agcttttacc gcccggcaat caaagcaaaa tgtcgtgatg
     3601 gattctgccc cattggcgaa accgtggctc tcagcaatgt cgataatctg accatctata
     3661 ccgagatcaa cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg
     3721 cgcagttgct gagcgccctg agcgaatttg ccacgctgaa tccaggcgac gccattctgc
     3781 tcggcacgcc acaggcgcgc gtggaaatac agccaggcga tcgcgttcgt gttctcgcag
     3841 aaggtttccc gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga
     3901 gcttcccaac gcagccacac ccgcacggca cgctgtttgc cctcggcctg aactacgccg
     3961 accacgccag cgaactggaa tttaagccac cggaagaacc gctggtattc ctgaaagcgc
     4021 caaataccct cactggcgat aaccagacct ccgtgcgtcc aaacaatatt gaatacatgc
     4081 actatgaagc ggagctggtg gtagttattg gcaaacaggc gcgtaacgtc agcgaagccg
     4141 atgccatgga ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgtgactatc
     4201 tggaaaacta ctaccgccct aacctgcggg taaaaagccg cgacggactg acgccgatgc
     4261 tttcaaccat cgtgccgaaa gaggcgatcc cggacccgca taatctgacc cttcgcacct
     4321 tcgtcaacgg cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct
     4381 tcctgatcgc ctacttaagt gaatttatga ccctgaatcc gggcgacatg atcgccaccg
     4441 gcacacccaa aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag
     4501 gcgtgggccg cctggtgaac cgaattgtga gtgaggatac agcgaaatga aaaaagtaaa
     4561 tcattggatc aacggcaaaa atgttgcagg taacgactac ttccagacca ccaatccggc
     4621 aacgggtgaa gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt
     4681 agcggcagcg aaagaggcgt tcccgaaatg ggccaatctg ccgatgaaag agcgtgcgcg
     4741 cctgatgcgc cgtctgggcg atctgatcga ccagaacgtg ccagagatcg ccgcgatgga
     4801 aaccgcggac accggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc
     4861 ccacaacttt gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt
     4921 tgacgacaag atgctcaact acacgctggt gcagccggtg ggcgtttgtg cgctggtatc
     4981 gccgtggaac gtaccgttta tgaccgccac atggaaggtc gcgccgtgtc tggcgctggg
     5041 caataccgcg gtactgaaaa tgtcggaact ctccccgctg accgctgacc gcctgggtga
     5101 gctggcgctg gaagccggta ttccggcagg cgtgctgaac gtggtacagg gctacggcgc
     5161 aaccgcaggg gatgcgctgg ttcgtgatca tgacgtacgt gccgtgtcgt tcaccggcgg
     5221 tacggccacc gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggaact
     5281 gggcggtaaa tcgccggtgc tgatttttga agatgccgat attgaacgcg cgctggacgc
     5341 cgccctgttc accatcttct cgatcaacgg cgaacgctgc accgccggtt cacgcatctt
     5401 tattcagcaa agcatctacc cggaattcgt taaacgcttt gccgaacgcg ccaaccgtct
     5461 gcgcgtgggc gatccgaacg atccgaatac ccaggttggc gcgcttatca gccagcagca
     5521 ctgggaaaaa gtctccggct atatccgtct cggcattgaa gaaggcgcca ccctgctggc
     5581 gggcggcccg gataaaccgt ctgacctgcc tgcacacctg aaaggcggca acttcctgcg
     5641 cccaacggtg ctggcagacg ttgataaccg tatgcgagtc gcccaagaag agattttcgg
     5701 gccggtcgcc tgcctgctgc cgtttaaaga cgaagctgaa ggcttacgcc tggcaaacga
     5761 cgtggagtac ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct
     5821 ggcgcgtggc attgaagctg gcatggtgtt cgtcaacacc cagaacgtgc gtgacctgcg
     5881 ccagccattt ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt
     5941 cgaagtgttc gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa
     6001 atggggagtc tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcga
     6061 tgtatctctc tgaactgcca gggaaaaacc acggttgccg ccagggcgcg atcgacgggc
     6121 ataaagagat cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata
     6181 cccactggct ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct
     6241 acaccagcaa cgagctgccg cattttatcc gcgatatgac ctacaactac gagggcaacc
     6301 cggagttggg gcagcttatt gccgatgaag ccttaaagct cggcgtgcgg gcaaaagcgc
     6361 acaacattcc cagcctgaaa ctggaatacg gcacgctggt gccgatgcgc tacatgaatg
     6421 aagacaagca cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg
     6481 acagccgcaa gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg
     6541 cggtccttgc cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcagaagaag
     6601 ggatgaacag ctacacccgc gagttcgacc gccagatgga cgagcgcgtg gtgaagttgt
     6661 ggcgcgaagg ccagttcaaa gagttctgca atatgctgcc ggagtacgcc gactactgct
     6721 acggcgaagg caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat
     6781 acgacggcaa ggtggagttt atcaccgagc tattcccaag ctctggcacc ggtcaggtta
     6841 acgctgtttt cccgcttccc gcgtaaggac gttttatgcc gcactttatc gttgaatgca
     6901 gtgacaacat ccgcgaagaa gccgacctgc cggggttgtt cgccaaagtg aatccgacgc
     6961 tggcagccac gggtattttt ccgctggcgg gtattcgcag ccgcgtgcat tgggtcgata
     7021 cctggcagat ggccgacggg cagcgtgatt atgccttcgt gcatatgacg ttgaaaatcg
     7081 gcgcaggtcg cagcctggaa agccgtcagc aggcgggtga aatgctgttt gaactgatta
     7141 aaacgcactt cgccgccctg atggagagcc gcctgctggc gttgtcgttt gagattgaag
     7201 agctgcatcc gacgctgaat tttaaacaaa acaacgtgca cgcattgttt aagtagcgcg
     7261 cagattgccc ggtggcgctg cgcttaccgg gcctacaaaa ccccaaaccg tacaccgtag
     7321 gccggataag gcgcagccgc atccggcaat gccacaggat atcgctatgt tcgataaaca
     7381 cacccacacc ctgatcgccc agcgtctgga tcaggcagaa aaacagcgcg aacagatccg
     7441 cgcgatctcg ctggattacc cagagatcac catcgaagac gcttacgcgg tgcagcgcga
     7501 atgggttcgc ctgaaaatcg ccgaaggccg tacgctgaaa ggccacaaaa tcggtctgac
     7561 ctcaaaagca atgcaggcca gctcgcagat cagcgaacca gattacggtg ccctgctgga
     7621 tgacatgttc ttccacgatg gcagcgatat cccgaccgat cgctttatcg tgccgcgcat
     7681 tgaagtggag ctggctttcg tgctggcaaa accgctgcgt gggccaaact gcacgctgtt
     7741 cgacgtctac aacgccacgg actacgtgat cccggcgctg gagctgatcg acgctcgctg
     7801 ccacaacatc gatccggaaa cccagcgccc gcgcaaagtg ttcgacacca tttctgataa
     7861 cgccgccaac gccggggtga tcctcggtgg tcgtccgatc aaaccagacg aactggatct
     7921 gcgttggatc tccgccctga tgtatcgcaa tggcgtgatt gaagaaaccg gcgtcgccgc
     7981 tggcgtgctg aatcatccgg caaacggcgt ggcctggctg gcgaacaaac tcgcaccgta
     8041 tgacgtacaa ctggaagccg ggcaaatcat tctcggtggt tcgttcaccc gcccggttcc
     8101 ggcgcgtaag ggcgacacct tccacgtcga ttacggcaac atgggttcca ttagctgccg
     8161 ctttgtttaa ggagagaacg atggaaaaca gctttaaagc ggcgctgaaa gcaggccgtc
     8221 cgcagatcgg attatggctg gggctgagta gcagctacag cgcagagtta ctggccggag
     8281 caggattcga ctggttgttg atcgacggtg agcacgcgcc gaataacgtg caaaccgtgc
     8341 tcacccagct acaggcgatt gcgccctacc ccagccagcc ggtagtacgt ccgtcgtgga
     8401 acgatccggt gcaaatcaaa caactgctgg acgtcggcac acaaaccttg ctggtgccga
     8461 tggtacaaaa cgctgacgaa gcccgtgaag cggtacgcgc cacccgttat ccccccgccg
     8521 gtattcgcgg tgtgggcagt gcgctggccc gcgcctcgcg ctggaatcgt attcctgatt
     8581 acctgcaaaa agccaacgat caaatgtgcg tgctggtgca gatcgaaacg cgtgaggcaa
     8641 tgaagaactt accgcagatt ctggacgtgg aaggcgtcga cggcgtgttt atcggcccgg
     8701 cggatctgag cgccgatatg ggttatgccg gtaatccgca gcacccggaa gtacaggccg
     8761 ccattgagca ggcgatcgtg cagatccgtg aatcgggcaa agcgccgggg atcctgatcg
     8821 ccaatgagca actggcaaaa cgctatctgg aactgggcgc gctgtttgtc gccgtcggcg
     8881 ttgacaccac cctgctcgcc cgcgccgctg aagcgctggc agcacggttt ggtgcgcagg
     8941 ccaccgccgt gaagcccggc gtgtattaat gccggatgcg ctgcacttat ccggcctaca
     9001 ctcgcaccaa accgtaggcc ggataaggcg tttacgccgc atccggcaaa aagccgtacc
     9061 aaatcgcgga ccggataagg cgttacgccg catccgccat aaaccttgtc gtaccctaca
     9121 aaaatcccat tagaggaaga aaaatgagcg acacctcacc tgccataccg gagagtatcg
     9181 atccggcgaa tcagcataaa gcgctgactg ccggacaaca ggcggttatt aagaagctat
     9241 ttcgccgcct gatcgtcttt ctgttcgtgc tgtttatctt ctcgttcctt gatcgcatca
     9301 acatcggctt tgccggactc acgatgggac gcgacctcgg tctgagcgcc accatgtttg
     9361 gcctcgctac caccctgttc tatgccgctt atgtcatctt cggcattccc agcaacatta
     9421 tgctgagtat tgtcggtgcg cggcgctgga tcgccaccat catggtgctc tggggcatcg
     9481 cctctactgc caccatgttt gccactggcc ccaccagctt gtacgtactg cgtatactgg
     9541 ttggcattac cgaagccggc tttctgcctg gtattctgct gtatttaacc ttctggtttc
     9601 cggcctattt ccgcgcccgt gccaacgcct tgtttatggt agcaatgccg gtaacgacag
     9661 cgttgggctc gatcgtttcc ggctacattt tgtcgctgga tggcgtcatg gcattaaaag
     9721 gctggcagtg gctgtttttg ctggaaggct tcccgtcggt attactcggc gtcatggtgt
     9781 ggttctggct tgatgactca ccggacaaag ctaagtggct gacgaaagaa gacaaaaaat
     9841 gcctgcaaga gatgatggat aacgatcgtc tgacgctggt tcagccagag ggagccatca
     9901 gccaccacgc catgcaacaa cgcagtatgt ggcgggagat cttcactccg gtggtgatga
     9961 tgtataccct ggcgtatttc tgcctgacca acacacttag tgcgatcagc atctggacac
    10021 cgcagatcct gcaaagtttt aatcagggca gcagtaatat caccatcggc ctgctggccg
    10081 ccgtaccgca gatttgtacc attctcggga tggtctactg gagccgtcac tcagatcgcc
    10141 gccaggaacg aaggcatcac accgcccttc cttatttgtt cgctgccgca ggttggttac
    10201 tggcttcggc aactgatcac aacatgatcc agatgctggg gatcattatg gcttcgaccg
    10261 gatcattcag cgcaatggcg attttctgga caacaccgga tcagtccatc agcctgcggg
    10321 cacgagcgat cggtattgcg gtgatcaacg ccactggcaa cattggttca gcgttaagtc
    10381 cgtttatgat cggctggttg aaagatttga ccggcagctt taacagtgga ttgtggtttg
    10441 ttgccgcgct gctggtgatt ggtgcgggga ttatctgggc gattccaatg cagtcctccc
    10501 gtccgcgagc gaccccgtaa ggaacgacga tgtgtgaccg tcagattgcc aatattgata
    10561 tcagcaaaga gtacgatgaa agcctgggca cggacgatgt gcattatcag tcgttcgccc
    10621 gcatggcggc attttttggc cgccatatgc tgccacatcg ccacgaacag tactttcaga
    10681 tgcatttcct caatagcgga cagattgagc tacagcttga cgatcatcgc tactcggtgg
    10741 aagcgcccct gtttgtcctg acgccgccgt cagtacctca tgcgtttatt acggagtctg
    10801 acgccgacgg tcatgtgttg acggtacggg aagatctgat ctggcccctg ctggaagttc
    10861 tttatccggg cactcgggaa accttcggcc tgccgggggc ctgtcactgg cagataaacc
    10921 cgacgaactg gcggcgctgg aacactattg gcaactgata gagcgggaat cggtggaaca
    10981 actgcctgga cgggaacaca ccctgacgtt actggcacag gcagtgttca ccctactgct
    11041 gcgtaacgca aaactcgacg accatgccgc cagcgggatg cgcggagaat taaaactgtt
    11101 ccagcgtttt aatatgctga ttgaaagcca ttttcatcag cactggacag taccggatta
    11161 cgctaacgaa ctgcatatca ccgaatcacg actcacggac atctgccgtc gctttgccaa
    11221 ccgtccgcca aaacggttga ttttcgacag gcagctgcga gaagccaagc ggctgctgct
    11281 gttttctgat aacgccgtga acaatattgc ctggcaactc ggttttaagg atccggctta
    11341 ttttgcgcgc ttttttaatc gcttagtcgg ttgctcgccc agtgcttatc gcgccaaaaa
    11401 agtacctgtg acgtgaaaat cccttagcct taacgggaaa ccaggcacca cctgctattc
    11461 ccctttctcc gcctggagca aggggattta gtaacgtcaa aaatcgctaa aagcgacctc
    11521 gatcacaaaa ccgtacccga cctgaaaagt accagccatt gtcccaaaag tctcttcccg
    11581 taatcgcccc tgcaagcttt aattgaataa caaaaacaac acacaaaatt aacaatcgta
    11641 ttccgattaa tactgtagag gtcgacatga aaccagaaga tttccgcgcc agtacccaac
    11701 gtccgttcac cggggaagag tatctgaaaa gcctgcagga tggtcgcgag atctatatct
    11761 atggcgagcg agtgaaagac gtcactactc atccggcatt tcgtaatgcg gcagcgtctg
    11821 ttgcccaact gtacgacgcg ctacacaaac cggagatgca ggactctctg tgctggaaca
    11881 ccgacaccgg cagcggcggc tatacccata aattcttccg cgtggcgaaa agtgccaacg
    11941 acctgcgcca gcaacgcgat gccatcgctg agtggtcacg cctgagctat ggctggatgg
    12001 gccgtacccc agactacaaa gctgctttcg gttgcgcact gggcgcgaat ccgggctttt
    12061 acggtcagtt cgagcagaac gcccgtaact ggtacacccg tattcaggaa actggcctct
    12121 actttaacca cgcgattgtt aacccaccga tcgatcgtca tttgccgacc gataaagtaa
    12181 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga
    12241 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
    12301 aagtgatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg
    12361 gcgtaaaatt aatctctcgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat
    12421 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
    12481 tgctgatccc atgggaaaac gtactgatct accgcgattt cgatcgctgc cgtcgctgga
    12541 caatggaagg cggttttgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
    12601 aattagactt cattacagca ctgctgaaaa aatcactcga atgtaccggc accctggagt
    12661 tccgtggtgt acaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
    12721 tgagtgactc gatgtgttct gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
    12781 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
    12841 ttatcgaacg caacgttacc agtggcctga tctatctccc ttccagtgcc cgtgacctga
    12901 acaatccgca gatcgaccag tatctggcga agtatgtgcg cggttcgaat ggtatggatc
    12961 acgtccagca catcaagatc ctcaaactga tgtgggacgc cattggcagc gagtttggtg
    13021 gtcgccacga actgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcagt
    13081 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
    13141 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cttgcacaac aacgacgata
    13201 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
    13261 acagcgcctg cgctttcgtg acgcgatggc cagcctgtcg gcagcggtaa atattatcac
    13321 caccgagggc gacgccggac aatgcgggat tacggcaacg gccgtctgct cggtcacgga
    13381 tacaccacca tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
    13441 gggcaacggt aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcacgcca
    13501 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
    13561 aggtccgctg gcgcagccgg tgctgaaagg ttcgctggcc agtcttgaag gtgagatccg
    13621 cgatgtgcag gcaattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
    13681 cagtgcagaa ggtcacggac ttatctactt taaacgccgt ttccatccag tgatgctgga
    13741 aatggaagct gcgatttaag ctccagcccc ttcacgtatt cgtcgaaggg gcatttttat
    13801 tgtgatctca atcacatatc cacccgatga ataattcttg cccaaccgct caccgccatt
    13861 cctgaccgct tacgtcccta aaataaccac tcagttattt accttacttt acgcgcgcgt
    13921 aactctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
    13981 ctagaacggc ttcggcctat gattaacaaa ttactctatg aattttggtt tgcaggaagg
    14041 cagcaagtga gtgaatcccc gggagcttac aacagtaagt gacagaggtg aacgaacgca
    14101 gctgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
    14161 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
    14221 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
    14281 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
    14341 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
    14401 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgccggtccg
    14461 ctggttggtc cggtgctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg
    14521 gcgggggtag tactggccgg tgcggttcag gactttatgg tgctgtttat ctcctctcgc
    14581 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tgggaccagt accggggact
    14641 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc tcgccgttct ggcgctgatt
    14701 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
    14761 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt aggtgaagtt
    14821 tctgttattg gtatcgtgct gctggttgcc tctatctatt tcggtggcgt gattgctcac
    14881 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
    14941 ggctatgcgt ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat
    15001 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtactg
    15061 aacccggaac tgaaaatgcc tgcaatgacc cagtacattg acggtactgg cccgctgtgg
    15121 aaaggcgctc tgttcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc
    15181 cacgcgctga tctcttccgg tacgacgccg aaattgctgg ctaacgaaac cgacgcgcgt
    15241 ttcattggct acggcgcaat gctgatggaa tccttcgtgg cgattatggc gctggttgct
    15301 gcgtccatca tcgaaccggg tctttacttc gcgatgaata ccccgcctgc tggccttggc
    15361 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
    15421 ctgaaagacg ttaccgcaca cgcggcggcg accgtcagct cctggggctt cgtgatttcg
    15481 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
    15541 ggtggcgcgc cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
    15601 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
    15661 gcgctggatg cgggtactcg ttctggccgc tttatgctgc aagacctgct gggtaacttc
    15721 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
    15781 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
    15841 agcctgtggc cgctgttcgg tatttccaac cagatgctgg cagccgtagc gctggtactg
    15901 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
    15961 gctgtatggc tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac
    16021 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
    16081 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc
    16141 aacgcaggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
    16201 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac tccgtacgtt
    16261 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccggttgc cggatgcgct
    16321 gcgcgtatcc ggcctacaaa gtgtctatcg taggccggat aaggcgttta cgccgcatcc
    16381 ggcaaatccc tagcccggac cttccgggct ttacgcatat ctggaaggca aaatgtttgg
    16441 taacttagga caggcaaaaa aatatctcgg ccaggcggcg aagatgttga ttggcattcc
    16501 agactacgac aactacgtcg agcatatgaa gaccaaccat cccgacaagc cgtacatgag
    16561 ctatgaagaa ttcttccgcg aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg
    16621 tatgcgctgt tgttaatgga gagagcatga acccgattgc agttacccta ctcaccggtt
    16681 ttttaggcgc gggcaaaacc accctgctgc gccatattct taacgaacaa cacggctaca
    16741 agattgccgt gattgaaaac gaattcggcg aagtctccgt tgatgatcaa ctgattggcg
    16801 accgcgccac gcagatcaaa acgctgacca acggctgcat ctgctgttcg cgctccaacg
    16861 agctggaaga cgcactactc gacctgctgg ataatctcga caagggcaat attcagttcg
    16921 accgtctggt cattgaatgc accggcatgg ccgatcccgg cccgattatt cagaccttct
    16981 tctcccatga gattttatgc cagcgctacc tgctggacgg ggtgattgcg ctggtggatg
    17041 cggtacatgc cgatgagcag atgaaccagt tcaccatcgc ccagtcccag gttggctacg
    17101 ccgaccgtat tctgctgacg aaaaccgacg tcgcaggcga agcagagaaa ctgcgtgaac
    17161 gcctggcgcg catcaacgcc cgcgcaccgg tctacaccgt tacccacggc gatatcgacc
    17221 tgggccttct gttcaacacc aacggtttta tgctggaaga aaacgtcgtc agcaccaaac
    17281 cgcgtttcca ctttatcgcg gacaaacaaa acgatatttc gtcgattgtg gtggaactgg
    17341 attacccggt agatatcagc gaagtttccc gggtgatgga aaacctgctg ctggaatcgg
    17401 cggataaact gctgcgttac aaagggatgc tgtggattga cggcgaacct aaccgcctgc
    17461 tgttccaggg cgtccagcgc ctctacagcg ccgactggga ccgcccgtgg ggcgatgaaa
    17521 aaccgcatag cacgatggtg tttatcggga ttcagttgcc ggaggatgag attcgggcgg
    17581 cgtttgcggg actcaaaaag taaccagccc tccaacatca caatacctga gactttatca
    17641 ccctcatatt cctccagaat atgagggcat tcgggcttaa ttatctctga acgtgcaaca
    17701 cttcctacga ctccttatgc gcctccacat cctgcttcgt taaccagagc gacttacgac
    17761 caaaattttc gtacattttc agctgatctt catagtgctg atcaactgtt ccatcgggag
    17821 caataaaccc actctgaccg ggtgcgacca catcccaggc aagcaccgga cgatcgcttg
    17881 tcgttggtga gaaaacaatc atatcgtttt ctgttccacg gttttgatac tccgcctgat
    17941 gacgcgtttc ttccgctgcg gcctgcggta catcaaagaa attatttgcc cggaacgtta
    18001 aggccattgc aggtgttttc cagttactca cattattgcc atagcgtttg gaaagagtct
    18061 cccaggtatc ttccagcgca gccaacacaa cctcctgctg tggtttccca gcaaacagat
    18121 caaccgcctg tgggattggt gatttgtctc cctgcaccgc ctcatacaaa atttttgctc
    18181 caacacttat attcagcgaa ccagttgggc cgtcctgggt tgtttcgtag ccactggcgc
    18241 tgtaccactt atcaaatggc ataggtacgg cagccactac ggtacgcttc aacatactgg
    18301 tcagccaaac gttcaggatg gcagagcctg
//
LOCUS       DAJTNN010000074        18084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTNN010000074 DAJTNN010000000
VERSION     DAJTNN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.074"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1612)
                     /gene="glnS"
                     /locus_tag="OO150_002202"
     CDS             complement(<1..1612)
                     /gene="glnS"
                     /locus_tag="OO150_002202"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5516341.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTRQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSSEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKP"
     gene            complement(1815..3761)
                     /gene="nagE"
                     /locus_tag="OO150_002203"
     CDS             complement(1815..3761)
                     /gene="nagE"
                     /locus_tag="OO150_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5516342.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4094..4894
                     /gene="nagB"
                     /locus_tag="OO150_002204"
     CDS             4094..4894
                     /gene="nagB"
                     /locus_tag="OO150_002204"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5516343.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            4954..6102
                     /gene="nagA"
                     /locus_tag="OO150_002205"
     CDS             4954..6102
                     /gene="nagA"
                     /locus_tag="OO150_002205"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5516344.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6111..7331
                     /gene="nagC"
                     /locus_tag="OO150_002206"
                     /gene_synonym="nagR"
     CDS             6111..7331
                     /gene="nagC"
                     /locus_tag="OO150_002206"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5516345.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYIGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7379..8131
                     /gene="nagD"
                     /locus_tag="OO150_002207"
     CDS             7379..8131
                     /gene="nagD"
                     /locus_tag="OO150_002207"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR5516346.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETSSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8528..10192
                     /gene="asnB"
                     /locus_tag="OO150_002208"
     CDS             8528..10192
                     /gene="asnB"
                     /locus_tag="OO150_002208"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5516347.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10573..10649
                     /locus_tag="OO150_002209"
     tRNA            10573..10649
                     /locus_tag="OO150_002209"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10607..10609,aa:Met,seq:cat)
     gene            10658..10742
                     /locus_tag="OO150_002210"
     tRNA            10658..10742
                     /locus_tag="OO150_002210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10692..10694,aa:Leu,seq:tag)
     gene            10766..10840
                     /locus_tag="OO150_002211"
     tRNA            10766..10840
                     /locus_tag="OO150_002211"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10798..10800,aa:Gln,seq:ttg)
     gene            10875..10949
                     /locus_tag="OO150_002212"
     tRNA            10875..10949
                     /locus_tag="OO150_002212"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10907..10909,aa:Gln,seq:ttg)
     gene            10965..11041
                     /locus_tag="OO150_002213"
     tRNA            10965..11041
                     /locus_tag="OO150_002213"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10999..11001,aa:Met,seq:cat)
     gene            11090..11164
                     /locus_tag="OO150_002214"
     tRNA            11090..11164
                     /locus_tag="OO150_002214"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11122..11124,aa:Gln,seq:ctg)
     gene            complement(11318..12493)
                     /gene="ubiF"
                     /locus_tag="OO150_002215"
     CDS             complement(11318..12493)
                     /gene="ubiF"
                     /locus_tag="OO150_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5516348.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12639..14063
                     /gene="miaB"
                     /locus_tag="OO150_002216"
     CDS             12639..14063
                     /gene="miaB"
                     /locus_tag="OO150_002216"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5516349.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPSETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14216..15256
                     /locus_tag="OO150_002217"
     CDS             14216..15256
                     /locus_tag="OO150_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5516350.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15253..15720
                     /gene="ybeY"
                     /locus_tag="OO150_002218"
     CDS             15253..15720
                     /gene="ybeY"
                     /locus_tag="OO150_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5516351.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15810..16688
                     /gene="corC"
                     /locus_tag="OO150_002219"
     CDS             15810..16688
                     /gene="corC"
                     /locus_tag="OO150_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5516352.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16713..>18084
                     /gene="lnt"
                     /locus_tag="OO150_002220"
     CDS             16713..>18084
                     /gene="lnt"
                     /locus_tag="OO150_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5516353.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGE"
BASE COUNT         4400 a         4765 c         4682 g         4237 t
ORIGIN      
        1 ccggtttttc cgctgtggaa tggcggctgt cgaggcagaa gtaaccttca cgctcaaact
       61 ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca
      121 ccagcgattc cgggttaatc accgacagga aatcatccgc agcacctggg ttaggcacgc
      181 tgaacagacg gtcgtacaga cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
      241 gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
      301 aagtacagaa gatggtggtg atattgcctt cggcatcttt ctccacgcgt tctgccttaa
      361 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
      421 tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc actgctaaac ggcacctggc
      481 ggctacccat ttccggtttg ttcggatggt tcggcatggt aaccatttcg ccttcgccct
      541 gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggggcgtttt
      601 cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgcc
      661 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc agcagtgtaa ccacgacgac
      721 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
      781 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
      841 cgtactggcg cgggtgaaca ggaatggtga tgttgtccag cacccagtcg tacaggcgac
      901 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagg gcatcgctga
      961 tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
     1021 cagcaaattt aatgcggtac agcaccggat cgcgcatcac gataaacggt gaagccatgt
     1081 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
     1141 acagcgccag gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggttgcg
     1201 tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag ttcatcaacg tacgccaggc
     1261 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
     1321 ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc gactcaacat
     1381 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
     1441 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc
     1501 cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg gccagatctt
     1561 catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
     1621 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcggaaca ggctgacaac
     1681 tgttagtttt ttaaaattga taatctaaca aggattatgg ggatgattga tgaaaaaaaa
     1741 agcggcagag aagatctcca ccgcttgtca ttgttggatg cgacgctcaa gcgtcgcatc
     1801 aggcataaag cagattactt tttgatttca tacagcggtg tttgacccgc cacaacatgg
     1861 ccctgagctt taatgatcaa gccactgaaa tcgtcgatat tgctgcaaac caccgggcta
     1921 atcatcgagc gggcgttagc gttcaggtaa tccagatcca tttccagaat cggttgccct
     1981 gcgcttacct gcgcaccctc ttccaccaga cgtttaaagc ctttaccttc cagcgctacg
     2041 gtgtcgatac ccatatggac gacgatctcc gcgccttttt cggtttccag gcagaacgcg
     2101 tggttggtgt tgaagatttt cacgattgtc cctgcggctg gtgatacgac gattttatct
     2161 gtcggtttca ccgccacacc gtcacccacc gctttgctgg cgaatgcttc gtcaggaacc
     2221 tgatccagtg ccacgacatc accggtaatc ggcgatacca gctccgcgat agataccgcg
     2281 tttggtacag cctgcggttt tgctacaggc gcggcagttg acggagttgc ttcagctgac
     2341 gcagcggcta ccggaccacg ggcaacgact ttcttcatcg catcgccgat ggattctgct
     2401 ttcgcgccaa caatcacctg aatagtctgt ttgttcagtt tcactacacc agaagcaccc
     2461 agacgtttac acatcgtatc gttgacgcgg gcagagtcag ccacggtcag gcgcagacgg
     2521 gtgatacagg cgtcaattgc tttcaggttg tcagtgccgc caaccgcagc aatatagttg
     2581 gtcgccagtt gattgaggcc ttcttcagtg ttgctgttgg cttcttcagt aacgatctca
     2641 tcttctttat cttcacgacc cggcgttttc aggttgaaca tgcggataac caaactgaac
     2701 accacgaagt agatagcgaa gaagacaaca cccatcacca gcagcatcca gacgttctgg
     2761 ctggcggccg gcaggttata catcaacgcg tagtcgatag cccccgcaga gaaagagaag
     2821 ccagcatgga tacccagcag tgttgccaca aacaggctga taccggtcag cagtgcgtgc
     2881 aggaggtaca gcagcggtgc aaggaacatg aacaggaatt ccagcggctc agtcacaccg
     2941 gtcaggaacg cagtaacagc aacagaaagc agcatcccgc caaccatcgg acgacgctct
     3001 ttcggtgctg cgaagtacat cgccagcgcc gcacccggca gaccgaacat cataatcggg
     3061 aagaagccgg acatgaacat ccccgcggtg ccgtcaccgg catagaagcg gttgatatca
     3121 ccgtggaaaa ccgtacccgc cgcgttggtg aattcaccaa tctggaacca ggcgatggtg
     3181 ttcagcacct gatgcagacc ggttgggatc agcagacggt tgatgaaacc aaagataccg
     3241 gaacccagcg cgcccgcaga aacgatccac tcgcctcctg catggatagc gtgctgtacc
     3301 ggcggccaga cgtaaccaaa aatggccgcc agcaccaggc agaagaagcc ggtggcaatc
     3361 ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg gcagtttaat atcggaccaa
     3421 cggttatagg ctgcgccacc aaccagaccg gtaatgatac ccgccagtac acccatgttt
     3481 atttctgggt tgatggtcac catcgctttg gttaacacaa agtaacctac cgcacccgcc
     3541 agcgccgccg cacctgcgct gtctttcgac cagctggatg ccacaccgat ggcgaagatt
     3601 aatgcgaggt tatcaaaaat cgcaccgccc gcctgggcaa taaacgcaac gttaagtaaa
     3661 tctggctgac cgaatcgcag caacagtgcc gccaccggca gcaccgcgat agggagctgt
     3721 aacgccctac cgagtcgctg gaaaaaacct aaaatattca tcttattccc cctacgagaa
     3781 ccctatttgg ctcgtttaaa gccgtatttt tattttgctg caaattgtac tgccgatgtt
     3841 ctgtaatcag attgttagat catctgctac agagtgtgtg aaaatttaat tcgtatcgca
     3901 aattaaacgc gtgtcttttg tgagttttgt caccaaatat cgttatcatc actccctttt
     3961 actggctaaa ccagaaaact tattttatca ttcaaaaaat caggtcggat tgacgcctgt
     4021 ctgcgcaaat ccaagttacg cttaaagatg cctaatccgc caacggctta cattttactt
     4081 attgaggtga ataatgagac tgatccccct gactaccgct gaacaggtcg gcaaatgggc
     4141 tgctcgccat atcgtcaatc gtatcaatgc gttcaaaccg actgccgatc gtccgtttgt
     4201 tctgggcctg ccaactggcg gcacgccgat gaccacctat aaagcgttag tcgaaatgca
     4261 taaagcaggc caggtcagct ttaagcacgt tgtcaccttc aacatggacg aatatgtcgg
     4321 tctgccgaaa gagcatccgg aaagctacta cagctttatg caccgtaatt tcttcgatca
     4381 cgttgatatt ccagcagaaa acatcaacct tctcaacggc aacgccccgg atatcgacgc
     4441 cgaatgccgc cagtatgaag aaaaaatccg ttcttacgga aaaattcatc tgtttatggg
     4501 cggtgtaggt aacgacggcc atattgcatt taacgaaccg gcgtcttctc tggcttctcg
     4561 tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt tctttgataa
     4621 cgatgtcaat caggtgccaa aatatgccct gactgtcggt gttggtacat tgctggatgc
     4681 cgaagaagtg atgattctgg tgctgggtag ccagaaagca ctggcactgc aggctgcagt
     4741 tgaaggttgc gtgaaccaca tgtggaccat cagctgtctg caactgcatc caaaagcgat
     4801 catggtgtgc gatgaacctt ccaccatgga gctgaaagtt aagactttaa gatatttcaa
     4861 tgaattagaa gcagaaaata tcaaaggtct gtaattgtta tccctgccct ggctccttgc
     4921 tcagggcaat attttttaaa atcgggggtc agaatgtatg cattaaccca gggccggatc
     4981 tttaccggcc acgaatttct tgatgaccac gcggttgtta tcgctgatgg cctgattaaa
     5041 agcgtctgtc cggtagcgga actgccgcca gagatcgaac aacgttcact gaacggggcc
     5101 attctctccc ccggttttat cgatgtgcag ttaaacggct gcggcggcgt acagtttaac
     5161 gacaccgctg aagcggtcag cgtggaaacg ctggaaatca tgcagaaagc caatgagaaa
     5221 tcaggctgta ctaactatct gccgacgctt atcaccacca gcgatgagct gatgaaacag
     5281 ggcgtgcgcg ttatgcgcga gtacctggca agacatccga atcaggcgtt aggtctgcat
     5341 ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa ttttgtgcgt
     5401 aagcctgatg ccgcgctggt cgatttcctg tgtgaaaacg ccgacgtcat taccaaagtg
     5461 accctggcac cggaaatggt tcctgcggaa gtcatcagca aactggcaaa tgccgggatt
     5521 gtggtttctg ccggtcactc caacgcgacg ttgaaagaag cgaaagccgg tttccgcgcg
     5581 gggattacct ttgccaccca tctgtacaac gcgatgccgt atattaccgg tcgtgaaccg
     5641 ggcctggcgg gcgcgatcct cgacgaagct gacatttatt gcggtattat cgctgatggc
     5701 ctgcatgttg attacgccaa cattcgtaac gctaaacgcc ttaaaggcga caaactgtgt
     5761 ctggttaccg acgccaccgc gccagcaggt gccaacattg aacagttcat ttttgcgggt
     5821 aaaacaatat actaccgtaa cggactttgt gtggatgaga acggtacgtt aagcggttca
     5881 tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac attgcggtat cgcactggat
     5941 gaagtgctgc gtatggcgac gctctatccg gcgcgtgcga ttggcgttga gaaacgtctc
     6001 ggcacactcg ccgcaggtaa agtagccaac ctgaccgcat tcacacctga ttttaaaatc
     6061 accaagacca tcgttaacgg taacgaggtc gtaactcaat aagagaaagt atgacaccag
     6121 gcggacaagc tcagataggt aatgttgatc tcgtaaaaca gcttaacagc gcggcggttt
     6181 atcgcctgat tgaccagtac gggccaatct cgcggattca gattgccgag caaagccagc
     6241 ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg ctgatcaaag
     6301 aagttgatca gcaggcctcc accgggggcc gccgcgctat ctccatcgtc accgaaaccc
     6361 gcaatttcca cgcaatcggc gtacggcttg gtcgtcacga cgccaccatc accctgtttg
     6421 atctcagcag caaagtgctg gcagaagaac attacccgct gccggaacgt acccagcaaa
     6481 cgctggaaca tgccctgttg aatgccattg ctcagtttat tgatagctac cagcgcaaac
     6541 tgcgcgagct gatcgcgatt tcggtgatcc tgccagggct tgttgacccg gacagcggca
     6601 aaattcatta catgccgcat attcaggtag aaaactgggg gctggtagaa gctctggaag
     6661 aacgttttaa agtgacctgt ttcgttggtc acgatatccg tagtctggcg ctggcggagc
     6721 actacttcgg tgcaagtcag gattgcgaag actccattct ggtgcgtgtc catcgcggaa
     6781 ccggggccgg gattatctct aacgggcgca tttttattgg ccgcaacggt aacgtcggtg
     6841 aaattggcca tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc aactttggct
     6901 gcctggaaac tatcgccgcc aacgctgcca ttgaacaacg ggtgttgaat ctgttaaagc
     6961 agggctacca gagccgcgtg ccgctggacg actgcaccat caaaactatc tgcaaagccg
     7021 cgaacaaagg cgatagtctg gcgtcggaag taattgagta tatcggtcgt catctgggta
     7081 aaaccatcgc cattgctatc aacttattta atccgcaaaa aattgttatt gccggtgaaa
     7141 tcaccgaagc cgataaagtg ctgctccctg ctattgaaag ctgcattaat acccaggcgc
     7201 tgaaggcgtt tcgcactaat ctgccggtgg tacgttctga gctggatcac cgctcggcaa
     7261 tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg tattttgctc cagcatttgc
     7321 tggaaaatta atgtgctttt atagtggcgc ttattgttgt caatattctg ggtagtccat
     7381 gaccattaaa aatgtaattt gcgatatcga cggcgtgctg atgcacgata acgtcgccgt
     7441 accgggtgca gcggaatttt tgcacgggat tatggataaa ggcctgccgc tggtgttgct
     7501 gaccaactat ccttcgcaga ctgggcaaga tctggcgaac cgctttgcca ccgcaggtgt
     7561 cgatgtacct gacagcgtgt tttatacctc tgcgatggcg actgccgatt tcctgcgtcg
     7621 ccaggaaggc aagaaagcgt atgtggtggg cgaaggcgca ctgattcatg aactgtacaa
     7681 agccggtttc actattaccg atgtgaaccc tgatttcgtg attgttggcg aaacgagttc
     7741 ctacaactgg gacatgatgc ataaagcagc ctatttcgtc gctaacggtg cacgttttat
     7801 cgccaccaat ccggacaccc acgggcgcgg tttttatccc gcgtgtggcg cgttgtgtgc
     7861 aggcatcgaa aaaatctccg ggcgtaaacc gttctatgtt ggtaaaccga gtccgtggat
     7921 catccgcgcg gcattaaaca aaatgcaggc gcattcggaa gaaacggtga ttgtcggcga
     7981 taacctgcgt accgatattc tggcgggctt ccaggcaggt ctggaaacga ttctggtgct
     8041 ttctggtgtt tcatcgctcg acgatatcga cagtatgccg ttccgcccaa gctggattta
     8101 cccgtcggtc gctgaaatcg acgttatctg aatagcaacc acgtctgcgg acgtggttgg
     8161 ctctgatgac aaacgccaaa ctgcctgatg cgctacgctt atcaggccta cgcagctcct
     8221 gcaatatatt gaattttcat gcttttgtag gccggataag gcgttcacgc cgcatccggc
     8281 atgaacaaat tgcactttgt cagcaatttg gcccccgtaa aatacaaaca ttcaataaaa
     8341 cccgctgaaa attatcgatt catcaataaa tatatcctca caccagacct tttttcacca
     8401 atcgacaatc accattacgt tttttatttt ttcaccgcca aatcgcttgc gcacagtgct
     8461 gatttgcggc atttttttaa acaagcaaac acaacaagca acaaatacca gattaacgga
     8521 gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa acagacgcag ttgagctgcg
     8581 taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact ggtccggtat
     8641 ttatgccagc gataacgcca ttctcgctca cgaacgtctg tcaattgttg acgttaacgc
     8701 gggggcgcaa cctctctaca accaacaaaa aacccacgta ctggcggtaa acggtgaaat
     8761 ctacaaccac caggcattgc gcgctgaata tggcgatcgt tatcagttcc agacagggtc
     8821 tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc tcgacgactt
     8881 gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa gatgcctacc tgattggtcg
     8941 cgaccatctg gggatcatcc cactgtatat gggctatgac gaacacggtc agctgtatgt
     9001 ggcctcagaa atgaaatcgc tggtgccagt ttgccgcacg attaaagagt tcccggcggg
     9061 gagctatttg tggagccagg acggcgaaat ccgttcttat tatcatcgcg actggttcga
     9121 ctttgatgca gtgaaagata acgtgaccga caaaaacgag ctgcgtcagg ctctggaaga
     9181 ttccgttaaa agccatctga tgtctgatgt gccttacggt gtgctgcttt ctggtggtct
     9241 ggattcctca attatttccg ctatcaccaa gaaatacgca gcccgtcgcg tggaagatca
     9301 ggaacgctct gaagcctggt ggccgcagtt acactccttt gctgtaggtc tgccgggttc
     9361 accggatctt aaagcagccc aggaagtggc aaaccatctg ggcacggtgc atcacgaaat
     9421 tcacttcact gtacaggaag gtctggatgc catccgcgac gtgatttacc acatcgaaac
     9481 ttatgatgtg accactattc gcgcttcaac accgatgtat ttaatgtcgc gtaagatcaa
     9541 ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct gatgaagtgt ttggcggtta
     9601 tctttacttc cataaagcgc ccaacgctaa agaactgcat gaagagacgg tgcgtaaact
     9661 gctggccctg catatgtatg actgcgcgcg cgccaacaaa gcgatgtcag cctggggcgt
     9721 ggaagcacgc gttccgttcc tcgacaaaaa attccttgat gtggcgatgc gcattaaccc
     9781 gcaggataaa atgtgcggta acggcaaaat ggaaaaacac atcctgcgtg aatgttttga
     9841 gtcatacctg cccgcaagcg tggcctggcg gcagaaagag cagttctccg atggcgtcgg
     9901 ttacagttgg atcgacaccc tgaaagaagt ggcggcgcag caggtttcta atcagcaact
     9961 ggaaactgcc cgcttccgct tcccgtacaa cacgccaacc tcaaaagaag cgtatctgta
    10021 ccgggagatc tttgaagaac tgttcccgct tccgagcgcc gctgagtgcg tgcctggcgg
    10081 tccttccgtc gcgtgttctt ccgctaaagc gatcgaatgg gatgaagcgt tcaagaaaat
    10141 ggacgatcca tctggtcgtg cggttggtgt tcaccagtcg gcatataagt aagaaatgcg
    10201 aaacagcccc ggtgaaatcc ggggctgttt cagttattga ggtgcttgtt gattctgata
    10261 tgcagtaagg aaatgttccg ggtttgccac atcactgttt gacaactcat cactgtaggc
    10321 cagtatttca tagattaatt tcccaccact attgttgaat tccgaatttc atcctgtacg
    10381 acaaaagaat ggctgtaggg tggggaaaaa acacaaagta aacaataatt gacgaatata
    10441 gcgccacgct gttcgcaacc taaccaaata gtcactttcg agcaattttc cttgaaaaag
    10501 aggttgacgc tgcaaggctc tatacgcata atgcgccccg caacgccgat aaggtatcgc
    10561 gaaaaaaaag atggctacgt agctcagttg gttagagcac atcactcata atgatggggt
    10621 cacaggttcg aatcccgtcg tagccaccat ctttttggcg ggagtggcga aattggtaga
    10681 cgcaccagat ttaggttctg gcgccgcaag gtgtgcgagt tcaagtctcg cctcccgcac
    10741 cattcaccag aaagtgttgt acggatgggg tatcgccaag cggtaaggca ccggtttttg
    10801 ataccggcat tccctggttc gaatccaggt accccagcca tcttcttcga gtaagcggtt
    10861 caccgcccgg ttattggggt atcgccaagc ggtaaggcac cggtttttga taccggcatt
    10921 ccctggttcg aatccaggta ccccagccat cgaagaaaca atctggctac gtagctcagt
    10981 tggttagagc acatcactca taatgatggg gtcacaggtt cgaatcccgt cgtagccacc
    11041 aaattctgaa tgtgtcgaat acgttcggca aattcaaaaa ccaatttgtt ggggtatcgc
    11101 caagcggtaa ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca
    11161 gccacattaa aaaagctcgc ttcggcgagc tttttgcttt tctgcgttca ttcaatgtcg
    11221 aatgcgatgt tgacacgtct tatccttcaa tgtcggatgc gacgctgccg cgtcttatcc
    11281 gacctacggt tggcacgcat ccggcaatgt tgtaaggcta caaccctaac gcatatttca
    11341 gcgcctgacg tttcaacacg ccagcacgct ctgccgccat taacccgaga ttacgcacaa
    11401 aacgcagcgg cggcagatta ttactgaatc cggcataaaa cagatccata ccactttgca
    11461 taatgaagtt atccgccatg cgccgcatct ggtaacgctt gaggacagga taactggccc
    11521 acgcttcacc gtaactgcgg gcattcacca gaacatcaat cagggcatcg acatcacgat
    11581 aaccaagatt caccccctgc cccgccagcg gatggatggt atgcgcggca tcgcccacca
    11641 gcgcaagccc cggctgcaca tactgcaacg catgtcggcg cgtcagcgga aacgcaccag
    11701 cggcaagcgg tgtaacgtaa cccagacgcg acgggaaatg cttcgcgatt tccgcctgga
    11761 gttgcgccat attcatattc tgcaactggc gaatacgcgc cggagagtca taccacacca
    11821 gcgatgccca gttatcaaac aacggcagaa acgcacgcgg tccgtccggg gtaaattgct
    11881 gccaggtgct gtcgccggga tcgttctcac actgtacgct aatcaacatg cacgactgcg
    11941 catactgcca ggcatgaacg ccaattcccg ccatctgccg cacctgagaa tttgcgccgt
    12001 cggcaccaat caccagtttc gcgcggatcg tttcaccgcc cttcagttcc agctcctgaa
    12061 gatcattatc tcgatgtaat gcaatcagcg agcctggcac acgtaacgtt actttcggat
    12121 gcgcttccag cgcctgccac agcgcctgtt gcaggacggt gttttccacc atatagccaa
    12181 gcaacggtag cttaagttca gcggcgtcaa acaccacatg cgccgtttcc cactcccacg
    12241 tttccagtct gcggtaagga tggcaacgca tagcctgtac tgcatcccag acccctaacc
    12301 ctttaagcaa tgataccgat gcggcgctga tcgccgagat ccgcacgtcc ggttggctat
    12361 cagcgacaaa cggtgcaggt tctgcgtgct cgatcaccgt taccgaaaat ccgtgctgtg
    12421 ccagccccag cgccagcgcg ccgccgacca ttcctccgcc gacaatggca atttccgttg
    12481 gttgatttgt catggtcaat catcctgtta gcaatatgct ttaagtttac aggattttta
    12541 cccccaaggg ctgataacgc tcatactggc cacaacggca gcaaagcatt acactatgcg
    12601 ccctgcattc ctggctacta tttcgcaaga gcaagtcgat gaccaaaaaa ctccatatta
    12661 aaacctgggg ctgtcagatg aacgagtacg attcatcgaa gatggccgat ctgctggatg
    12721 ccacccacgg ctatcaactg accgacgtgg cggaagaagc ggatgtgctg ctgctgaaca
    12781 cctgctcaat ccgcgagaag gctcaggaaa aagtcttcca tcagttgggt cgctggaaac
    12841 tgttaaaaga gaagaatcca gacctgatta tcggcgtcgg tggctgcgtg gcatcgcaag
    12901 aaggcgagca cattcgccag cgcgcccact atgtcgatat tatttttggg ccgcaaacgc
    12961 tgcaccgtct gccggagatg atcaactccg tgcgcggcga ccgcagcccg gttgtagata
    13021 tcagcttccc ggaaatcgag aagtttgacc gtctaccgga accgcgcgcc gaagggccga
    13081 ccgcgtttgt ctccatcatg gaaggctgca ataaatattg cacctactgc gtggtgcctt
    13141 acacccgtgg tgaagaggta agccgtccgt ctgacgatat tctgtttgag attgcccagc
    13201 ttgcggctca gggcgtgcgt gaagtcaacc tgctcggtca gaacgtgaac gcctggcgtg
    13261 gtgaaaacta cgacggctcc accggatcgt ttgccgatct gctgcgtctg gttgctgcga
    13321 tcgacgggat cgatcgtatt cgctttacca ccagccatcc gatcgaattc accgacgata
    13381 tcatcgaagt gtatcgcgac acgccggagc tggtgagctt cctgcatctg ccggtacaga
    13441 gcggttccga tcgcattctg aacctgatgg gccgtactca tacggcactg gagtacaaag
    13501 cgatcatccg taaactgcgt gcggcgcgtc cagatattca gatcagttct gatttcatcg
    13561 ttggcttccc tagcgaaacc accgaagact tcgagaaaac gatgaagctg attgccgacg
    13621 tcaatttcga catgagctac agctttatct tctctgcacg tccgggtaca ccagccgccg
    13681 atatggttga tgatgttccg gaagaagaga agaagcagcg tctgtatatt ctgcaagaac
    13741 gtatcaatca gcaagcgatg gcatggagcc gccggatgct cggcaccacc cagcgtattc
    13801 tggtagaagg gacttcgcgt aagagcatca tggagctttc cgggcgtacc gaaaataacc
    13861 gcgtggtcaa cttcgagggc acaccggata tgatcggtaa attcgtcgat gtagaaatta
    13921 ccgacgtcta cccgaactct ctgcgcggta aagtggtgcg tactgaagat gaaatgggcc
    13981 tgcgcgtggc agaaacaccg gaatcggtga ttgcccgtac ccgcaaagaa aacgaccttg
    14041 gcgtgggtta ttatcagccg taattctcag gcccgccgtt ccggtgggcc ttgttttttc
    14101 tctctttatc cccatcttta tcgcaatgct tgcgcctttt tcctgtggcg tgaataattt
    14161 ccttataggc cggtgaatgt tcagcttccg gcggcataca gataagagga acggtttgaa
    14221 catagacact cgcgaaatca ccctggagcc agcagacaac gcgcgtctgt tgagcctgtg
    14281 cggcccgttt gatgacaaca tcaagcagct cgaacgccgt ctcggcatcg agatcaatcg
    14341 ccgcgacaac cactttaaac tgaccggccg tccgatttgc gtcaccgctg cggcagacat
    14401 tctgcgtagc ctgtatgtcg atacagcccc gatgcgcggt cagattcagg atatcgaacc
    14461 ggaacagatc caccttacga ttaaagaagc gcgagtactg gagcaaagtg cggagagcgt
    14521 gccggagtac ggcaaagcgg tcaatatcaa aaccaaacgc ggcgtaatta agccgcgcac
    14581 gccaaaccag gcgcagtaca tcgccaatat tctcgaccat gacattacct tcggcgttgg
    14641 cccggcgggt acgggtaaaa cctacctggc agtggctgcg gcagttgatg ccctggagcg
    14701 tcaggagatt cgccgtattc tgctgactcg tccggcggtc gaagccggtg agaaactggg
    14761 cttcctgcct ggcgatttaa gccagaaagt agacccgtat ctgcgcccac tgtacgacgc
    14821 gctgtttgaa atgctgggct ttgagaaagt tgagaaactg attgagcgca acgttattga
    14881 agtcgcgccg ctggcctata tgcgtggtcg tacgctgaac gacgcgttta tcattctcga
    14941 tgagagccag aacaccacca tcgaacagat gaagatgttc ctgacccgta tcggttttaa
    15001 ctcaaaagcg gttatcaccg gcgacgtcac acagatcgac ctgccgcgta atactaaatc
    15061 aggcttacgt cacgccatcg aagtactggc cgatgtcgaa gagatcagct ttaacttctt
    15121 ccacagcgaa gacgtggttc gtcaccctgt ggtggcgcgt atcgttaacg cctatgaagc
    15181 ctgggaagaa gccgaacaaa aacgaaaagc ggcgctggcg gcagaacgca agcgcgaaga
    15241 acaggaacaa aaatgagtca ggtgatcctc gatttacaac tggcatgtga agataattcc
    15301 gggttaccgg aagagagcca gtttcaaaca tggctgaatg cggtgatccc gcagtttcag
    15361 gaagaatcgg aagtgactat tcgcgtggtc gataccgccg aaagccacag tctgaatctg
    15421 acctatcgcg gtaaggataa gccgaccaac gtgctctcct tcccgtttga agtgccgcct
    15481 ggcatggaaa tgtcgctact gggcgatctg gttatctgcc gtcaggtggt tgagaaggaa
    15541 gctcaggagc aaggcaaacc actggaggcg cactgggcgc atatggtggt gcacggcagt
    15601 ctgcatttgt taggttacga tcacatcgaa gatgacgaag cagaagaaat ggaagccctc
    15661 gaaacagaga ttatgcttgc tctgggctat gaggatccgt acattgccga gaaagaataa
    15721 tttgccaggc atttgactgg cagacgtccc cgccgttggt gattaacgac ggcgttttga
    15781 ttaactaaca tgagaaccca tacgacgcca tgagcgacga caattcacac agtagtgaca
    15841 ctataagcaa caagaaggga tttttctccc tgttactcag ccaacttttc cacggtgaac
    15901 cgaaaaaccg tgacgaactg ctggcgctga tccgtgattc cgggcagaac gaccttatcg
    15961 acgaagatac gcgcgatatg ctcgaagggg tgatggacat cgcagaccaa cgcgttcgcg
    16021 acatcatgat cccccgctcc cagatgatta ccctgaaacg caaccagacg ctggacgaat
    16081 gccttgatgt catcatcgag tccgcccact cacgtttccc ggtgattagc gaagacaaag
    16141 atcacattga agggattctg atggcgaaag acttgctgcc gtttatgcgc agcgatgctg
    16201 aagccttcag catggacaaa gtgttacgtc aggcggttgt cgttcctgaa agtaagcgcg
    16261 tagaccggat gctgaaagag tttcgctctc agcgttacca catggcgatc gtcattgacg
    16321 aattcggtgg ggtttccggt ctggtgacca ttgaagacat cctggaactg attgttggtg
    16381 agattgaaga cgaatatgac gaagaagatg atatcgactt ccgtcagctg agtcgtcata
    16441 cctggaccgt gcgcgcactg gcttccattg aagacttcaa cgaagcgttc ggcacccact
    16501 ttagcgatga agaagtcgac actatcggtg gtctggtgat gcaggcgttt gggcatcttc
    16561 cggcgcgtgg cgaaactatc gacatcgacg gttaccagtt caaagtggcg atggccgaca
    16621 gtcgacgtat tattcaggtt catgtcaaaa tcccggatga ctcaccccag ccgaagctgg
    16681 atgaataaaa ccgaaactgg atagataact acatggcttt tgcctcatta attgaacgcc
    16741 agcgcattcg cctgctgctg gcgttattat tcggtgcctg cggaacgctg gccttctctc
    16801 cttacgacgt ctggcctgcg gcgattattt cgttgatggg gcttcaggcg ttgaccttta
    16861 accgccgtcc actccagtct gccgctattg gcttttgctg gggatttggc ctctttggca
    16921 gcggtattaa ctgggtctat gtcagcatcg cgacctttgg cggaatgcct ggcccggtta
    16981 acatcttcct ggtggtgctg ctggcggcgt atttgtcgct gtataccgga ctgtttgctg
    17041 gcgtgctgtc gcgtctgtgg ccgaaaacca cctggctgcg cgtggcgatt gccgcccctg
    17101 ctctctggca agtgaccgag tttctgcgcg gttgggtact gacaggcttc ccgtggttac
    17161 agttcggcta tagccagatt gatggcccgt taaaagggct ggcaccgata atgggcgtgg
    17221 aagccattaa cttcctgctg atgatggtta gtggcctgct ggcactggcg ttggtcaaac
    17281 gcaactggcg tccgctggtg gtggccgtcg tgctgtttgc cctacccttc ccgctgcgtt
    17341 acatccaatg gtttaccccg caaccggaga aaaccattca ggtttcgatg gttcagggcg
    17401 atattccgca atcactgaaa tgggatggag atcagcttct taatacgctg aagatttact
    17461 acaacgcaac ggctccgctg atgggcaaat catcgttgat tatctggccg gagtcggcga
    17521 taaccgatct ggaaattaat cagcaaccgt tcctcaaagc actggacggt gagttgcgcg
    17581 ataaaggtag ctcgctggta accgggattg tcgacgcgcg tctcaataag cagaaccgct
    17641 acgataccta caacaccatc atcacactgg gtaaaggtgc accgtacagc tacgaatcag
    17701 ccgatcgcta taacaaaaac catctggtgc cgtttggcga gtttgtcccg ctggagtcga
    17761 ttctgcgtcc gttagcaccg ttctttgatc tgccgatgtc gtcgttcagc cgtgggccat
    17821 atatccagcc gccgctgtca gtaaatggta ttgagcttac tgcggctatt tgctacgaga
    17881 tcattctcgg cgagcaagtg cgcgataact tccgcccgga taccgactat ctgctgacta
    17941 tctccaacga tgcgtggttt ggtaaatcta ttggtccatg gcaacacttc cagatggcgc
    18001 gaatgcgtgc gctggagctg gcgcgcccac tgttgcgcag caccaacaac ggcattacgg
    18061 cggtgattgg cccgcagggt gaga
//
LOCUS       DAJTNN010000075        17692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTNN010000075 DAJTNN010000000
VERSION     DAJTNN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.075"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..224)
                     /locus_tag="OO150_002221"
     CDS             complement(<1..224)
                     /locus_tag="OO150_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCR5516354.1"
                     /translation="MTWNLLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLR"
     gene            complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="OO150_002222"
     CDS             complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="OO150_002222"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5516355.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="OO150_002223"
     CDS             complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="OO150_002223"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5516356.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="OO150_002224"
     CDS             complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="OO150_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5516357.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4373..5671)
                     /gene="purA"
                     /locus_tag="OO150_002225"
     CDS             complement(4373..5671)
                     /gene="purA"
                     /locus_tag="OO150_002225"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5516358.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5775..5972)
                     /locus_tag="OO150_002226"
     CDS             complement(5775..5972)
                     /locus_tag="OO150_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5516359.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="OO150_002227"
     CDS             complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="OO150_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5516360.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="OO150_002228"
     CDS             complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="OO150_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5516361.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTYPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="OO150_002229"
     CDS             complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="OO150_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5516362.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETILADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="OO150_002230"
     CDS             complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="OO150_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5516363.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="OO150_002231"
     CDS             complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="OO150_002231"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5516364.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAITG"
     gene            complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="OO150_002232"
     CDS             complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="OO150_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5516365.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="OO150_002233"
     CDS             complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="OO150_002233"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5516366.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="OO150_002234"
     CDS             complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="OO150_002234"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5516367.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="OO150_002235"
     CDS             complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="OO150_002235"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5516368.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHSITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16292..17431
                     /gene="queG"
                     /locus_tag="OO150_002236"
     CDS             16292..17431
                     /gene="queG"
                     /locus_tag="OO150_002236"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5516369.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQPICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGGH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17414..17467)
                     /locus_tag="OO150_002237"
     CDS             complement(17414..17467)
                     /locus_tag="OO150_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516370.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         4068 a         5011 c         4394 g         4219 t
ORIGIN      
        1 tttcttaata agaaggtatt aaattcatct ggattgctga tactacttac cggacaaata
       61 aaggtttcga taatcatttg gcgggaagta aaaagaatat tgatttgcaa atcgccataa
      121 tcattcattt taataattaa tgcgttctcg ctatttgtta catcaatatt ttgttcaggt
      181 acagtttgca gcgccgtcgc cagcgccaac agattccatg tcatatatta ttccatatag
      241 atttagttta aatattaata aaatgaatat ttgcagtacg taattatctt accagctata
      301 gacaaaaaaa aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc
      361 tgacgcaccg cttcaaataa gcaaattccg gttgcaaccg aaacgttcag agaagaaaca
      421 cttcctgcca ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata
      481 ccttcacctt ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt
      541 gtatgatccg cctcacctgc cgtaccgacg atccagatat tctcttcctg caacatacgc
      601 atggtacgca ccaggttagt cacccgaatc agcggaacgc tttctgccgc gccacaggcc
      661 acttttttcg ccgtggcgtt gagctgggcg gagcgatctt tcggcacaat caccgcatga
      721 acaccagcgg catctgcgct acgcaggcac gcgccgaggt tatgcggatc ggttacaccg
      781 tcaaggatca gcaggaacgg ctgatcgagc gaagcgatca gatccggcaa atcattttcc
      841 tggtactgac gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt
      901 ttctcgtcga gatattggcg gtttgccagc tggataacca cgccctgggc ttcgagggcg
      961 tgaatcagcg gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa
     1021 cgttcagggg cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc
     1081 attaatgtac tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat
     1141 ctctgcaata tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc
     1201 cggcatgaac aaagcgcatt ttgtcagcaa tctaaccctc ttctgtttga agagggtaat
     1261 gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc agctattttc
     1321 tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc tttcggcttc
     1381 gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgaccaga
     1441 cgacgcttgc cgcctttttt acctgcatcg ccttttttcg ctttctcgcg cgccgtttta
     1501 ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt gcgctcgtcc
     1561 atattaaccg cttcgacgcg aacttccacg cgatcgccca gacgataagt ctggccgctg
     1621 gattcaccca tcagccgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa
     1681 gagacatgga ccagaccatc aatgaataag tcgtccagac ggacaaagaa tccaaagcca
     1741 gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac
     1801 ttcagccagt cagccacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag
     1861 tgctgaccca gttgcagcat ttcttccatc gaataatggt agccgccggt ttcagtggtg
     1921 ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg
     1981 tctggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc caggccaaag
     2041 tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc
     2101 agcatttctg catcaggacg atcggcaacc gactccagca gctccgcgta gtcacgcggt
     2161 tccggcttgt taccgcccgg cagttccagc cccagctccg ccagtactga acggaaagag
     2221 gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca atgctggttc tttggctttc
     2281 tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat taacttgtgt
     2341 gcgtcgttac gctgagtctg ttcgatacgc tcaatacggc attcagcgtt gaaaataaac
     2401 ttcgcttctt cgctctcaaa tgagatcccg ccacgttctt cacgggcttt atccagcact
     2461 ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gagcgtactg ctcgcgcaga
     2521 tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc
     2581 atcaccgctt cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg
     2641 cacaccatac acaggcggtc tacctgcgga ttgagcgagc acagaccgtt agagagcact
     2701 tccggcagca tagggataac ctgcgaaggg aagtacaccg acgtaccacg attacgcgct
     2761 tctctatcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat
     2821 aaacgccagc cgccgccgcg ttttttctcg cagtaaaccg catcgtcaaa gtcacgggcg
     2881 tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct
     2941 tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg cggccagatg
     3001 tacggaattt catgggtacg caaagcgata tcaaccgcca tgccggtgcc catattgtcg
     3061 cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg ttgggtcagt
     3121 tcaacaacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata
     3181 tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag
     3241 cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga
     3301 cctttacggt cagcacccag cggctgagcc agcacctgat cgccatgaat gcaggttttc
     3361 atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag
     3421 ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag
     3481 cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc
     3541 aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg
     3601 gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc
     3661 gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg
     3721 tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct
     3781 cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct
     3841 tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat
     3901 tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg
     3961 gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac
     4021 taagttgatt gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt
     4081 cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc
     4141 cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata
     4201 catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga
     4261 aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca
     4321 atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt
     4381 cgaacgggtc gcgcagaatc atagtttcag tacgatccgg accggtagag atgatatcga
     4441 tcggcacacc ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcagcc
     4501 cgctacgatc tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg
     4561 gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg
     4621 gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag
     4681 tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca
     4741 gccagccggt acgacgacga cgacccgtcg ttgcgccgaa ttcgttaccc tgcttgcaga
     4801 ggaactcgcc agtttcatca aacagttcgg tcgggaaagg acctgcaccc acacgagtgg
     4861 agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg
     4921 tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga
     4981 tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct
     5041 ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg
     5101 tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga
     5161 agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt
     5221 cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac
     5281 cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agcgcaacgt
     5341 gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc
     5401 cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt
     5461 taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg
     5521 ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa
     5581 catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt
     5641 gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg
     5701 gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc
     5761 agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca
     5821 accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag
     5881 atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc
     5941 agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca
     6001 ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc
     6061 gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc
     6121 acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag
     6181 aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca
     6241 ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg
     6301 gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc
     6361 gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg
     6421 atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg
     6481 atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg
     6541 ttatctgctg ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg
     6601 tcacgtactt cgagggtcag acgaccacgg gaatcagtga cgatatcttt cacgtccagg
     6661 cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc
     6721 gaaatgtcgc caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg
     6781 atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg
     6841 tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa
     6901 tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa
     6961 cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc
     7021 agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt
     7081 agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg
     7141 acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcaaacgc agcagattgc
     7201 tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg
     7261 gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca
     7321 ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt
     7381 ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct
     7441 tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt
     7501 cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg
     7561 catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca
     7621 gcagagtgat gcccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgggtatcgc
     7681 tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataaccc
     7741 cgcgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca
     7801 gatatttttc cggataggta acgcggtact gcacgttcat ctcaacgcgc actacgttct
     7861 cgtccgacgt cagcatcaca ccggaagcgg ccagttcacg cacggcttcc acgtttaccg
     7921 gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga
     7981 atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg
     8041 ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg
     8101 ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac
     8161 caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc
     8221 gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt
     8281 cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt
     8341 atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta
     8401 cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca
     8461 acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct
     8521 attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg cagacgcaat
     8581 gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc
     8641 cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca
     8701 atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc
     8761 tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc
     8821 acatccgccg catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg
     8881 gctttaaatg ccgccaccag atcgtgcggc agatggcgaa taaaccctac ggtatctgca
     8941 agtatggttt caccgacatc cgcgacgtca atacgccgca acgtcgggtc gagggtggca
     9001 aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta
     9061 ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc
     9121 cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga
     9181 ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc
     9241 ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc
     9301 agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata
     9361 tctaaaataa ggccggtgcg gtcgatgaca cgacactcgc acaaacgctc caggttacgc
     9421 tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt cgctttgaca
     9481 gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta
     9541 cggctaccgg taatcacctg caatgcttcg acaccggcgg aagaaaccag agattcaaac
     9601 tcctggaggt cttccatatc tttgtctttc gtaaaataga tgtgtaccag taccgcctgc
     9661 tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg
     9721 caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgg tttcttcgct
     9781 gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc
     9841 gccaccggcg ttgttactgt gatgagaaac cggacgagac gggacaacag tagaaatcgc
     9901 gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga
     9961 ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc
    10021 ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt
    10081 tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg
    10141 tctcaattgt acacattcag cctgtgatag caccaacaac ctgtaatact tcgtcacgcg
    10201 cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta
    10261 tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg
    10321 agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca
    10381 agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag
    10441 ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg
    10501 cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg
    10561 ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat
    10621 ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact
    10681 cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg
    10741 gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca
    10801 gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat
    10861 cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga
    10921 gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag
    10981 aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt
    11041 taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat
    11101 cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc
    11161 ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc
    11221 tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata
    11281 ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt
    11341 tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct
    11401 gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt
    11461 tcttcgccag aaacttttag ccgcaacgga ataagcagcg gctgagcgca aactggcgct
    11521 tcacccgggg tcagttgtac ctgacgcagc caacgttctg ccactggcaa ggctaaaagt
    11581 gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga
    11641 ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttaatttt
    11701 tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag
    11761 cctggctgcg cattcggcca aggggcagcc ggacgactgc ctgatgctgg cgcaggagtg
    11821 tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc
    11881 acgcggtttt ccggaatgga acgcggtgca ggttggggtt catcgtccag cggtagcggc
    11941 gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga
    12001 cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgca cgttgacgtc cacctgatgc
    12061 ggatcgatct ccagatacag cacaaatgcc ggttgctgat cggcccccag tttgtcttcg
    12121 caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc gttcacgtag
    12181 caatactgaa tttctgccag tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgt
    12241 agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcaagaaa agcggtgccg
    12301 caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc
    12361 acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg
    12421 cggatgatct catcaatgtg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc
    12481 gccggggtgt tgtagaacag atccagcacc tccagcgtcg tccccacagg atgcgccgcc
    12541 ggttttaccg tcacgttcat atcgcgccct tcggcatagg cctgccaggc ttcctgctgt
    12601 tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca
    12661 ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg
    12721 gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga
    12781 atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca
    12841 tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct
    12901 gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag
    12961 tttggcagcg tgcgatctgg cgtcgtcacc gtactggccg tttgtgccgt tgcaccctgc
    13021 ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta aatggcttct
    13081 gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata
    13141 aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc gagactcgcg
    13201 tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat catactggtc
    13261 gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt
    13321 aaatagggat cagactgact gttcgccagc acatcgcccg ccccacccag cagctccgaa
    13381 tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg gttagagagc
    13441 acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg aatcgacacg
    13501 aggaaattag cgttttgctt acgtgccaca tcgctgcgcc ccatcaccga aataaagtaa
    13561 tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat
    13621 ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca
    13681 gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc
    13741 gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta
    13801 ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc
    13861 gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta
    13921 atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttacca
    13981 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    14041 atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt
    14101 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    14161 ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag
    14221 agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg
    14281 atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    14341 tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    14401 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    14461 cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg
    14521 gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata gggttcgacc
    14581 agcgtataag tggggctttt gacattaccc tgatgaccca gagcttgtaa aaagccccgg
    14641 ctaaaggtgg tcttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    14701 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    14761 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    14821 aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc
    14881 cgcaccgtgc gcgacacagc ccgcacaggc ggcatcatac ggcgacagtt tttgcccaag
    14941 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    15001 tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    15061 cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact
    15121 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat
    15181 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    15241 caacatcggt ttgcgaaaat tctcaacttt ttgcagtgct tttttccccc actcttgctg
    15301 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc
    15361 catcgtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg caatgttttc
    15421 actgcgggtc agtactcgga ctaaaccagc accagcacgc agcgccgctt cccccgtcat
    15481 acgaatagcc cccgccgtgc cgtgatcgcc accgataatt accagccgcc cgtgatcgcc
    15541 tttatgcgaa gtcgggcgac gcggttttag ccagtgagaa agttgttctg ctgaaaaccg
    15601 ctgaattttc gtctcctgac ctgccagcca actatccagc cccagtgagt caaaatgcag
    15661 ttgtccggta acatcccgcg cttttccagt gagcaagcct ggtttcagcg caataaaagt
    15721 gatggagtga tcggcgttga tcaccgcgcc tggcgtagcg ccagtttcag ccagcaggcc
    15781 ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg
    15841 gctaatggat tcgcggggcg cttgccgcaa accggtaccg agcagcgcat caacaatcag
    15901 atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca
    15961 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggcaaacaa
    16021 cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt
    16081 attaccatga ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac
    16141 ctggaatgcg gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc
    16201 tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact
    16261 tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt
    16321 agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga
    16381 taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg
    16441 cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgccctcatg agttattgcc
    16501 cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc
    16561 cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta
    16621 tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt
    16681 ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga
    16741 aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc
    16801 gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga
    16861 ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga
    16921 gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc
    16981 gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca
    17041 gcctatctgc ccgtggaatc gctattcaca actcaccaca gaagaggatt tcagcccgcg
    17101 taagccgcta cacgcaccgg aactcattga gttattcgcc tggagcgaag agaagttttt
    17161 aaaagtcacg gaaggatcgg cgattcgtcg aattggtcac ctgcgttggc tgcgtaatat
    17221 cgccgtagca ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg
    17281 taaaggtggg cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga
    17341 gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt
    17401 gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa
    17461 aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc
    17521 ttaattttta acttatctat caataggtta aaaaaatact attcaccgtg cgaagtttct
    17581 ttgaaattaa gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag
    17641 acaacgaaaa ccagcaataa cgcatcgctg tggatatttt attgagagaa ga
//
LOCUS       DAJTNN010000076        17495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTNN010000076 DAJTNN010000000
VERSION     DAJTNN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.076"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..734
                     /locus_tag="OO150_002238"
     CDS             <1..734
                     /locus_tag="OO150_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5516371.1"
                     /translation="KPAKLKFHQYARATLLSNRGRDHNCDGRRTVEIHKLDLSDWQAF
                     NKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLH
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(805..1254)
                     /gene="rplI"
                     /locus_tag="OO150_002239"
     CDS             complement(805..1254)
                     /gene="rplI"
                     /locus_tag="OO150_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5516372.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1296..1523)
                     /gene="rpsR"
                     /locus_tag="OO150_002240"
     CDS             complement(1296..1523)
                     /gene="rpsR"
                     /locus_tag="OO150_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5516373.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1528..1842)
                     /gene="priB"
                     /locus_tag="OO150_002241"
     CDS             complement(1528..1842)
                     /gene="priB"
                     /locus_tag="OO150_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5516374.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1849..2244)
                     /gene="rpsF"
                     /locus_tag="OO150_002242"
     CDS             complement(1849..2244)
                     /gene="rpsF"
                     /locus_tag="OO150_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5516375.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2571..2846
                     /locus_tag="OO150_002243"
     CDS             2571..2846
                     /locus_tag="OO150_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5516376.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2921..3472)
                     /locus_tag="OO150_002244"
     CDS             complement(2921..3472)
                     /locus_tag="OO150_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR5516377.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3569..4255)
                     /gene="ulaF"
                     /locus_tag="OO150_002245"
     CDS             complement(3569..4255)
                     /gene="ulaF"
                     /locus_tag="OO150_002245"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5516378.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4255..5109)
                     /gene="ulaE"
                     /locus_tag="OO150_002246"
     CDS             complement(4255..5109)
                     /gene="ulaE"
                     /locus_tag="OO150_002246"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5516379.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5119..5769)
                     /gene="ulaD"
                     /locus_tag="OO150_002247"
                     /pseudo
     CDS             complement(5119..5769)
                     /gene="ulaD"
                     /locus_tag="OO150_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
     gene            complement(5783..6247)
                     /gene="ulaC"
                     /locus_tag="OO150_002248"
     CDS             complement(5783..6247)
                     /gene="ulaC"
                     /locus_tag="OO150_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5516380.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6257..6562)
                     /gene="ulaB"
                     /locus_tag="OO150_002249"
     CDS             complement(6257..6562)
                     /gene="ulaB"
                     /locus_tag="OO150_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5516381.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6578..8026)
                     /gene="ulaA"
                     /locus_tag="OO150_002250"
     CDS             complement(6578..8026)
                     /gene="ulaA"
                     /locus_tag="OO150_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5516382.1"
                     /translation="MSAQHSGCAEYPVTSSSGDRLYNIFTVFFNQVMTNAPLLLGIVT
                     CLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISD
                     TYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGL
                     IAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASW
                     IAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAM
                     AGKVNWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAA
                     IYSFAPNAVVWGFMWGTIGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFG
                     GWRAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMA
                     VIIVIALAYMFFAGRALRAEEDAEKQLAEQSA"
     gene            8330..9394
                     /gene="ulaG"
                     /locus_tag="OO150_002251"
     CDS             8330..9394
                     /gene="ulaG"
                     /locus_tag="OO150_002251"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5516383.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVQWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9502..10257
                     /gene="ulaR"
                     /locus_tag="OO150_002252"
     CDS             9502..10257
                     /gene="ulaR"
                     /locus_tag="OO150_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5516384.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10254..11003)
                     /gene="yjfP"
                     /locus_tag="OO150_002253"
     CDS             complement(10254..11003)
                     /gene="yjfP"
                     /locus_tag="OO150_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5516385.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11185..11514
                     /gene="bsmA"
                     /locus_tag="OO150_002254"
     CDS             11185..11514
                     /gene="bsmA"
                     /locus_tag="OO150_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5516386.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11663..11938
                     /gene="yjfN"
                     /locus_tag="OO150_002255"
     CDS             11663..11938
                     /gene="yjfN"
                     /locus_tag="OO150_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5516387.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12055..13680)
                     /locus_tag="OO150_002256"
     CDS             complement(12055..13680)
                     /locus_tag="OO150_002256"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5516388.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSLLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13764..14822)
                     /locus_tag="OO150_002257"
     CDS             complement(13764..14822)
                     /locus_tag="OO150_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5516389.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
                     QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQW
                     VEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGN
                     VTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIRED
                     NTLITKDTSRFVPHYIAG"
     gene            complement(14825..15463)
                     /locus_tag="OO150_002258"
     CDS             complement(14825..15463)
                     /locus_tag="OO150_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5516390.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWLNTSGDYSWRSGSGKKESYSSGGFTTKKASTISRGGYGRSSSARGHWGG"
     gene            complement(15473..15871)
                     /locus_tag="OO150_002259"
     CDS             complement(15473..15871)
                     /locus_tag="OO150_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5516391.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15889..16548)
                     /locus_tag="OO150_002260"
     CDS             complement(15889..16548)
                     /locus_tag="OO150_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCR5516392.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16599..17297)
                     /locus_tag="OO150_002261"
     CDS             complement(16599..17297)
                     /locus_tag="OO150_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCR5516393.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
     gene            complement(17370..>17495)
                     /locus_tag="OO150_002262"
     CDS             complement(17370..>17495)
                     /locus_tag="OO150_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCR5516394.1"
                     /translation="NQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT"
BASE COUNT         4467 a         4672 c         4184 g         4172 t
ORIGIN      
        1 acaaaccagc aaaattaaaa tttcatcaat acgcacgcgc tacattactg tcaaatcgtg
       61 gaagagatca taattgcgat ggcagacgta ctgttgagat ccacaaacta gatctctctg
      121 actggcaagc attcaataag ttagcaacga agtgtaatgc ttacgatgga gtaacaatga
      181 atggtgataa ttcttttgga tggaaccatg aagctacatt agataatatt cacgcccaga
      241 aatacaataa agcttacgcg ggtgcgaggt taaccgcaga actgaaatat ttattgcaag
      301 atgtcgaatc cttcgaaccc aacagtaaat acacaataca cgaagtggtt ttgggacctg
      361 gttatggtac tccagactat accggccaaa cgataggata cgttgtaacc cttcctgccc
      421 aaatgcctaa ttgctggtca tctgaactac caacaatcga tctttatatc gaccaacttc
      481 acactgttac aggcgtttct aatgccctcg gattcatcat tgctgcattg ctaaacgcgt
      541 attctgattt accccatgat ttgaaaattg gtctgcgttc cttatcaaat tcagcagcaa
      601 tctactcggg tttaggtttc gagcgagtgc cgcaggaccg agacataagg agtgatcgta
      661 tgcatctaac gccagcaaac catcctgact tatggacaca agagaatggt gaatggatct
      721 acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa cgccgaccaa tggtcggcgt
      781 ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca ctttcgcgaa
      841 tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca gaacgccgtt
      901 cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag cgtcagcgat
      961 gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag acgcgatggt
     1021 aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca gaacttcagc
     1081 cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt tcttggtagc
     1141 tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt taacgtttac
     1201 ctgatcacct aggctaccca ggtttgctac tttatcaagc agaataactt gcattacctt
     1261 atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc agtgtacggc
     1321 agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg gtattttgca
     1381 cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta gtttttcagc
     1441 gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg gcagaacttg
     1501 cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt caatctgctc
     1561 ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa tgaacccctg
     1621 aacggttatg cgactgccga ccgttatact gtgagtaatg gcctggtttt cgtgtccgct
     1681 aacaataacg ggcatttggc accacgcctg ccggtgaaaa ccggcttcct cctgcacaga
     1741 acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct ttcgaagggg
     1801 agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt actcttcaga
     1861 atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac gctcacggcg
     1921 ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg tacgcataac
     1981 catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat cgatcacttc
     2041 ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt tgatcgggta
     2101 agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg caccagtgat
     2161 ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag gatggaccat
     2221 aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag cctcctgtct
     2281 gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa aaatgacgcg
     2341 ttatagtact tgtatgcccc actaaactca aggccagccg acaaataaat ctcatctttc
     2401 gctcaaaaga actaactcgc tgatttgaag ttcatcacat tccgtcacag tgttttttcg
     2461 aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa attaaatcag
     2521 aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct atgttcagtc
     2581 gtgttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca gccattgaaa
     2641 ttaataacca ccaggccaga aatatggacg acgtacagag cttaggcgtg atttatatca
     2701 atcataattt cgccactgaa agcgaagcac gtcaggcatt aaatgaagag acagatgcgc
     2761 agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac ggcaatatgc
     2821 acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat tgccatagtt
     2881 tgctttgccc ccttcgcagg gggctttttt atacggttat tcaccggatt gcgacttccg
     2941 gatctggctc atgagtaatg cgattacgta agtcgcggcg cataatttct attatccaga
     3001 accagaaaat atgccccagg atttcagagg catattcgtc aaaaggcaat tgagtgagcg
     3061 gcggcgttaa attcagcgcc gggcaaaata taccgtggac tgccactgtc acaataagcc
     3121 ctgctaatat cccctgccat aatttcactt tcgggaaaat ttccgcgaca atacaatacc
     3181 ctatcgcaaa aaccagtgag aagatgatat gcgtcaccat caccgggttg ataatatgtt
     3241 ccgagaatgt atacacagtt tgggttgggt cgatacctaa ataatcccgt agaaagatat
     3301 acggcgggtt aaattcatcg cgaccaccgg aaaatgtgcg cggtggcaag gggacttccg
     3361 ctccccattt aacaaatgct gaaataattc ctgaaagtac acccacaagt aacgcaacgc
     3421 catagcggcg acgttgcgga tccgttctga caaacaatcc attgatagcc ataaaaacac
     3481 ctgttgttta tacgattttt taactgaagc ggccccggaa tcagaccaat tacatcccgc
     3541 aggaggtaat aattccgggg cgcactgctt acttctgccc gtaataggcg ttaggaccgt
     3601 gcttacgcat gaagtgttta ttcatgagga agctgtcgat gtgattcagt tgtgggttaa
     3661 tactgcgcgc aatccacgcc attttcgcca cttcttccat caccaccgcg ttatgcaccg
     3721 cgtcgtgggc atctttcccc caggcgaacg gcccgtgctg atacaccaca attcccggcg
     3781 tatgcagcgg ctcggcgtca cccagcgttt cgataatcac tttgccggtg ttcagttcat
     3841 actcgccctg cacctcttct tcgcttaatc cacgcgtaca cgggatgtcg ccaaagaagt
     3901 agtcggcgtg cgtggtgcct aacgccggga tcgccagccc cgcctgcgcc catgcggtgg
     3961 catgagtgga atgggtatgg acaatgccgc cgagcgacgg gtaacgacgg tagagttcaa
     4021 gatgcgtcgc ggtgtcagaa gacgggcgat acttcccttc taccaccttg ccgctcatat
     4081 cgaccaccac catatcgtct gctttcatgg tttcgtaggc aacgccgctg ggcttgatca
     4141 ccaccagccc gcgttcgcgg tcgatagcgc tgacgttgcc ccaggtaaag gtcaccagcc
     4201 catagcgcgg cagatccatg ttggcttcaa acacctgctg ttttagcttt tgcattatgc
     4261 cgcctccacc atgcctgctt tggccatgcg cgctttcacc caatcacgcg ctttcaccac
     4321 ttctgccgcc gggtcttccg ccgtttcgct ccacatctca atcaggtacg gcccgcaata
     4381 gccactctgt ttgagcgttt cgaagcaacg ttcgaaatcc accacacctt cgccaaatgg
     4441 cacgtttttg aagacgcccg gtttggtgtc tttcacatgc accgcgacga tatgcccgat
     4501 tccggcctgc aactccatct gcacatcgtt gtcccacgcc gacaggttgc cgatatctgg
     4561 gtagagctgg aaccacgggt tgttgagata gtgcgcgtaa cccagcgcct tgctgatgga
     4621 gttcatcaac ggataatcca tgatctccat cgccagcgtc acctgcgcgc ggctcgccat
     4681 ctcaacgctc tctttcaggc cgtcacggaa acgacgacgc gtttcgttat tggcttcctg
     4741 atagtaaacg tcatagcccg ccagctggat cacgcgaatg ccaacgtcct gggcgaactg
     4801 gatggcttta cgcataatct ccagcccctg cgcccgcacc gcgtcatctt cgctgcccaa
     4861 cgggaaacga cgatgggcag aaaggcacat ggacggcaca cgcacgccgg tttcaacaat
     4921 cgcattcacc agcgccagac gctgctcgcg gctccagtcg aggcgcgaca ggcgctcgtc
     4981 agtttcatct accgacatct cgacaaaatc gaagcccaac gtttttgcca gtcgcaggcg
     5041 ttccagccag cactccccgg cggggagcgc tttttcatag atgccaagcg ggatttgttt
     5101 ggacaacata tccgctcctt agccccacag ttcggcgatg gaacgtttga actgacgcgc
     5161 ggcttcaacc ggagaagcgg catcacggat gctgcgaccg gcgataaaca catgaattgg
     5221 aatgcctttg aacagcggca gatcttccag cgccaggcca ccggtgacgg tgactttgaa
     5281 gcccatatcg gaaagacgtt tgatcgcagt gatatcggct tcaccccacg ccacgccagc
     5341 ggcctgcgcg tcacggctgc ggtgataaac cacctgctga atacctgcgt cgcgccactg
     5401 ttacgcctgt tcccaggtcc agtaaccggt cagttcgatc tgcacgtcgc cgttaaactc
     5461 ttttgctacg tccagcgcac ctttggcggt gttgatatcc gcacaacaaa tcacggtcac
     5521 ccagtcagcg ttggcttcga agcacatacg agaaagaatt ttgcccgcat cggcaatttt
     5581 ggcgtctgcc agcacgattt tgtgcgggta gagcgctttc agatcgcgaa ccgcacgcac
     5641 gccttcgccc acgcacagaa tggtacccac ttcgataatg tcgacttctt cggcaatcag
     5701 gcgagtggtt tcgtaggcgc tatccatagt ctggttgtcc agcgcgactt gcaacatcgg
     5761 taatgacatc ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg agatccagta
     5821 cttcctgctc ggtacggcag gcacgtaaac ggtcgaaatt ttcttcatct tcaaacaggt
     5881 tgacgatctg catgatgccc acttcctggt gcgtgttggc atcgaccgcc gccatggtga
     5941 tgaggatatc caccgggtcg ttatcttcgt ggttgaactc cagcggcttt ttcagcgtca
     6001 ccagcgcaaa accggttttc ttaacgccct cttccgggcg accgtgcggc attgccaggc
     6061 ctggtgcgag cacaaagtac ggaccgtgct gttcaaccgc atccagaatc gcctggtagt
     6121 aacgcggctc gacaacatcc gccgccacca gcaggtcaac gccgattttt actgcgtcct
     6181 gccatgtctc agcttcagcc tgcaggcgga tggatttatt ctccgccagc gaatcacgta
     6241 atttcatggc gcgtccttac ttcacatcct gcgggaaatg cgctttgatc acttccagca
     6301 gtttcgggcc aaagtcggca ggagagagca tgttgcgcac gccaaccacg tatttgttgc
     6361 cggtcacggt gatttcgccc gcaatgtgcg tagaagcgat gatgatatcc gcgccgctca
     6421 actcgctttt gtattcgcca accgcgcagc tatttaccgt gtggtcaatg tttgattggg
     6481 ttaaaaactg gtccactttc atcttcatga tcatggaact gccttgtccg ttgccacaca
     6541 cagccagaat acgtacggtc ataatcaaaa ctccttatta agcagactgt tctgccagtt
     6601 gtttttctgc atcttcttct gcgcgcagtg cgcggccagc gaagaacata taagccagtg
     6661 caattacaat gatgacggcc ataaaggcga taccgatgga gaagaagccc tgcatcatcg
     6721 gcggtgccag aatcgaccag tccgccatgc ccatccaggc gctcataccg gtgagtttca
     6781 ccgcccagac gcagccaaag atttcaatca tccccatcac cagacatatc ttcagcgccg
     6841 cacgccagcc gccgaagtgg ttagcgaaca cgccgatggt ggcgttggag aagaacatcg
     6901 ggataaagcc gggaataatc aggatcgagg aaccgcaggc gaccaggatg ccaaccgcaa
     6961 tcagctgacc gatggtgccc cacataaagc cccaaaccac ggcgttcggc gcgaagctat
     7021 agatagccgc acagtcaatc gccagaaccg caccagggat caggcgctgg gagataccgt
     7081 taaatgcttc agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg atgaagatcg
     7141 ccaccgcgaa ggagaaacca gtttgcagga tgtacaccgt ccagttcact ttgcctgcca
     7201 tcgcctgcac ggtgtcgata ccgaaggaga gtagaatggc accaaagaag atggtcatca
     7261 caatcgccgt ggagacgatg ttgtcgtgga aaatattcag ccagcctggc agtttgaggt
     7321 cttcaacgct ttcctctttt ttgccgagga acggcgcaac tttataggca atccatgatg
     7381 caaactgttg ctggtgaccg atggagaaac cgcagccgtc cgtcacttcc tgagtcggct
     7441 tgtacatcat attggaggta atgccccagt agagcgaaac cagaatcgcc gtgcagataa
     7501 tggttgtcca catggagtag ccgaaaataa acaacgtaac ggcaatcagc cccgcctgct
     7561 ggaacatgat atgaccagtc agcatgatgg tacgaatacc ggtaatgcga cgcagcagca
     7621 cgtaacagat gttcagcgcc agcgctaaca acaccgcgta acctacccag ctataggcat
     7681 cgcccatgcg gtcgatggtt gccatcatcg acgcgtaagt atcggaaatt gcgccgttaa
     7741 tgccgtagac ttcggacatt ttcgccacca ccggtttgaa ggtgctggtg aggatgccgg
     7801 accctgcctg caacaacatg aaaccaatta tggttttaat cgtgccttta ataataacgc
     7861 tgacactttt gcgcagtagg atgtagccca gacaggtcac aatacccagc aacaacgggg
     7921 cattggtcat gacctggtta aaaaacacgg taaagatgtt gtagagacga tctcctgaag
     7981 aagaggtaac cgggtattcc gcacacccgg aatgttgtgc actcactcta attttcaaaa
     8041 gcaatcacaa caagattatt tatgattaaa tgtgacgcga cccgcaaata attccatacg
     8101 gatagagtgg gatccacaca acgaaacaat tatttattta aatttagtta aattcattta
     8161 gttaatttaa ctatacattc cattaaatct ttccacactc aaaatcaaca acaacttccc
     8221 tggcaaaaag tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag gattaatcaa
     8281 aaccaatcac atattgattc gaattgaact atcatgaagg taaatggcga tgagtaaagt
     8341 gaaaagtatc acccgagaat cctggatcct gagcactttc ccggagtggg gtagctggct
     8401 gaatgaagaa attgaacaag agcaggtcgc ccctggcaca tttgcgatgt ggtggctagg
     8461 ctgcaccggg atctggttga aatcagaagg tggcaccaac gtttgcgttg atttctggtg
     8521 cggcactggc aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc agatgcagcg
     8581 catggctggc gtgaaaaaac tgcagccaaa cctacgcacc accccgtttg ttcttgatcc
     8641 gtttgcgatt cgccagatcg acgcggtact ggcgactcac gatcacaacg atcatatcga
     8701 cgttaacgtc gctgctgccg tgatgcagaa ctgtgctgat gacgtaccat tcattggacc
     8761 gaaaacctgc gttgatttgt ggattggctg gggcgtaccg aaagagcgtt gcatcgtggt
     8821 caaaccgggc gatgtggtga aagtgaaaga cattgaaatt catgcgcttg atgctttcga
     8881 ccgtactgca ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac tgccggacgg
     8941 catggacgat cgcgcggtga actatctgtt caaaacgcct ggcggctccc tgtatcacag
     9001 cggcgactcc cactactcta actattatgc gaagcacggt aacgaacatc agatcgacgt
     9061 ggctttaggt tcatacggcg aaaacccgcg cggtatcacc gacaaaatga ccagcgccga
     9121 tatgctgcgt atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc accacgatat
     9181 ctggtcaaac ttccaggccg ctccgcaaga gatccgcgtg cagtgggaga tgaaaaaaga
     9241 tcgtctgaag tatggcttca agccgtttat ctggcaggtt ggcggcaaat ttacctggcc
     9301 actggataaa gacaacttcg agtaccacta tccgcgcggt ttcgatgatt gcttcactat
     9361 tgaaccggat ctgccgttca aatcattcct gtaatctgtc gtttataccg gatagcgtgg
     9421 agaagttcgc gctatccggc gtagtatttc ctggctattt caaatatcat ctaaaaaaat
     9481 caaattttat cggaatagct catgactgaa gcacaaagac atcaaatcct cctggaaatg
     9541 ctcgcacaat tgggctttgt gaccgttgag aaagtcgttg agcgtctggg aatttcgcct
     9601 gccactgcgc gacgcgatat caataaactt gacgaaagcg gcaaactcaa aaaggtgcgc
     9661 aatggcgcag aagctattac ccaacagcgc ccgcgctgga cgccgatgaa tctgcatcag
     9721 gcgcagaatc acgatgaaaa agtacgtatt gctaaagcgg cctcgcagct ggttaatccg
     9781 ggcgaaagcg tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg ggaaatgtgt
     9841 ggcaagccag tgcaaatcat cactaattat ctaccgctgg caaattacct gatcgatcaa
     9901 gaacatgaca gcgtgatcat tatgggtgga cagtacaaca aaagtcagtc catcacttta
     9961 agcccgcagg gcagcgaaaa cagtctctat gccgggcact ggatgtttac cagcggaaaa
    10021 gggctgaccg cagaagggtt gtataaaacc gatatgctga cagcaatggc agagcagaag
    10081 atgctgagcg tggtagggaa actggtggta ctggttgata gcagtaagat tggcgaacgc
    10141 gcgggaatgc tttttagccg tgccgatcaa atcgatatgc ttatcaccgg caaaaatgct
    10201 aacccggaaa tcctgcaaca actggaagcg caaggggtca gcattctgcg tgtttaaaga
    10261 tgctggcgga aaaatgtcac ggcagcatct aacgcctcag gcgtaatgcg gtgacgcacg
    10321 cctggctgcc atgaacaggt tagcagttta tcccgtcccg tctcgcttaa ggcctgctgc
    10381 aaacgtagtg attcgtcggc aggcacaacg tcatcgtcga ggccatgcca cagcagtaga
    10441 ggtctgtccc caagttgttc caggtggttt gtcgcttccc actctgccag cggcgcgata
    10501 atgttattga attcattctg ctgtgctgcc gtttcaggta tcagcggtgg aaacagtgaa
    10561 cgggcgagcg atgtaaaata gcccgatccc atcatgctgg cggtacattt caccgtgggg
    10621 tggcgagcgg taattcccag tgccgtcatc gcgcccatcg acgcgccacc gactgccaga
    10681 cggtcatcaa gcagccagtt ttcttcggct attgccgcac gtaaagtagt gaattcctgc
    10741 atactttgta gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc gctactaaaa
    10801 cggctaccgt gatcgggcgc atccggcatg atcacccgca aaccagcctg cgccagcgca
    10861 acggcaaaat agctatacac caggctggat gaagtaaagc cgtgataaaa aattacgcac
    10921 ggtaacgggg tatctttttg cccgacagga taagcatgaa gaacgggaat atctgccagc
    10981 tcgcgtgatt ctatttcaat catctgtctc tcctttttgt ctggtatgac atgaagaaat
    11041 ccttagcaca agtttctgct ggcggtatta gttcataatg ttgagatgtg ggttacgctt
    11101 tcgtcacgtt ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca ttcccccaaa
    11161 gttaaattaa taaggcacta cactatggtt agcaggaaac gtaatagcgt tatttaccgg
    11221 tttgccagtt tattattggt gttgatgtta agtgcctgta gcgcactgca aggtacgcca
    11281 cagccagcac cgccagtaac gaaccatccg caagagattc gccgcgacca gacgcaaggg
    11341 ttacagcgaa taggtagcgt aagcacgatg gttcggggtt ctccggatga cgcattagca
    11401 gaaatcagag caaaagctgt cgctgcaaaa gctgattatt acgttgtcgt catggtggac
    11461 gaaaccatcg tgacaggaca gtggtattca caagccattt tgtaccgtaa ataaatcaga
    11521 caacttacat agtcagcttt acattgcttt gcgtccctat gcgtttacct gtgcacaata
    11581 aattaccggc tggaagcctg ggcgtttatt accgcgatgg aatgccctgc aacatgtggg
    11641 gaaacgaaaa atggagctga cgatgaaaca attacttgct tcaccctcgc tgcaattagt
    11701 gacttatcct gcgagcgcca cggcacagtc tgcagaattc gccagtgctg actgcgtgac
    11761 gggcctgaat gaaataggtc agatctcagt tagcaatatt tcaggggatc cgcaggacgt
    11821 ggaacgtatc gtggcgttaa aggccgatga acaaggtgcg tcatggtacc gcattattac
    11881 catgtacgaa gaccagcagc ctgataactg gcgcgtacag gcgatactgt atgcatgagt
    11941 gtcatctaca gcaaatgaac aatgccggat tcggcgcgag cgccttgtcc ggcctacaat
    12001 tttcacatat ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat acgcttacac
    12061 acacactccc cccgtcgccc gcagcaataa atcattctgg atctgctctg acaaccgtac
    12121 gccgccgcgc gtatctaaca tcacctgaca ccacgcctgc gccattggcg gagaagcata
    12181 tttcaacatt tgcgcaccgc agcccagcag gaatagctga tgagtaatct ctcgccccag
    12241 ttcttcagct ggcttacgca gctgctgctg taaacgacga accgcgcgat caaaatagcg
    12301 atcctgccct ttcacttcca caaatgcttc cgacaataag tcgtatacgc ccgcttgctt
    12361 attgagaacg cgcaacacat ccaggcacat aatattgccg gaaccttccc aaatactgtt
    12421 taccggcatc tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac caatgccgcc
    12481 cagcacctcc atcgcttcgg ccacaaacgg catacctctt ttgcagatca caaattttgc
    12541 cgcaggcgta aataaacgcg cccacagggc ttctttggca tcggcacgcc ggtcccacgc
    12601 tcgcgcaaga cgaaacagca acgccgtttg cccttcaagc tgaagtgcca tgcgacttaa
    12661 gacatgacac ataaggggct gttggatcaa tggattacca aaaacatggc gttgatgtgc
    12721 atgataaatc gccagcgaaa atgcacggcg catcatggca tggctaccca gggcgcaatc
    12781 aaaacgcgtc atcccaccca ttttcaggat cagacgaatt ccttcccctt cctgccccaa
    12841 caaccagcca atggcatcct gaaactccac ttcgcaactg gcgttagagc gattacccag
    12901 cttatctttc agccgctcga ggcgaatcgc gttgcgttgc ccatcaggca aaaagcgcgg
    12961 cacaaaaaag caggacaaac cgcccgtagt ctgcgccagc accagatgcg catcgctttg
    13021 cggcaccgag aaaaaccatt tatgccccac cagccgataa gagccatctt ccagacgctc
    13081 tgcacgggtg gtgttgctca tgacatcgga accgccctgc ttttccgtca ttcccatgcc
    13141 aatcaacaaa ccgcgttttt gcccacctgg caataagaga gaatcgtagc gatcgctcag
    13201 cagcggcgtg gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca atggcgtggc
    13261 ggcaaaggtc atcgttattg gacataacga cccagcctca acctgcgcat gtaacataaa
    13321 acgcgccgcg cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg caagattgtg
    13381 cacccgattg gtacataacg cctgcatcag caggtgccag gcggggtgaa aacgcacatc
    13441 gtccagacgg cgtccttgcg catcgtagcg caataattca ggcggattca cattcgccag
    13501 ccgccccagt tcaagagatt cagccgttcc taactgctga ccaatactgg ctagaaaatc
    13561 gctatcccag ccagcacctt cacgcgttac cgcttcacag agcgcgccat cagacaggta
    13621 taagttgctg ttatttaatg gtataggttg attaaaaacg gtgtgagttt gccagtgcac
    13681 tgtgtctccc tccatcaatg gcagtcaccc ttagtatggt cactgccatg gattcctgac
    13741 agaaagagat tgctaaaaca ttcttatcca gcaatgtaat gcggaacgaa acgtgaggtg
    13801 tctttggtga tcagcgtgtt atcttcacgg atccccattc cgcacgcttc atcatcgaca
    13861 atccagctac cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg aaacgcctgg
    13921 tagatcatcg gttcatcggc gtaatcacca tcagcgtggt caacgacgtt attctgaccg
    13981 tcgaaaatgg tgacgtttcc gccttcgcgt gagtagatcg gtttacgcac atagctttcg
    14041 ccagcggcaa tctgcggttt ttcgccctca aaccaggatg caagaaggtt aggatgacca
    14101 ggaaagaagc gccaaagcag cggcattagc cctttattac tcaagatact tttccataac
    14161 ggctccaccc attgctcgcg acgcttgcgc agcagcggac cgttatcgtc acgcatcatc
    14221 cactccagcg gatacagctt aaatgcacgc tggatgacat tatcatcaag atcggtcagt
    14281 acgccgccga cgcccaaacc gagatcttca atgtagataa accgcgactc ctgccctgcc
    14341 tgctgggcgc agtcctgcaa gtacagcacg gtactcctgt cttcatcggt gtcctgacag
    14401 cagcaaaaat aaaacggttc ccgactgtaa agctcactaa agcgcgaaat caggcgttcc
    14461 tgaatggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc aaaatccatc
    14521 cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc ccagtacaac
    14581 ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc caccacctca
    14641 aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat ctggcgcaga
    14701 gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat atgaaagtcg
    14761 aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt gtgtctcagc
    14821 atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg ccgcgcgaaa
    14881 tggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt ttgccagaac
    14941 cggagcgcca ggagtaatca ccggaagtgt tgagccagac agggcgggaa gcaaatgagg
    15001 atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataacc cggcttaaca
    15061 aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag caattttcgt
    15121 acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa aatgccgctt
    15181 tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca atacaatcct
    15241 gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccacta tcgccacaac
    15301 ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa cgttaagtat ttttgtgcgt
    15361 agcgattgcg acgcggttca aaaggattat tcggtctacc aatgcgactg atcgctccgt
    15421 gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc cattaccatg
    15481 tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata aacattcctg
    15541 ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt ctgacaccag
    15601 caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag tctggaatac
    15661 tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat gtaccgctga
    15721 atgccagtga caccgcggta ttattgtttt tgattaattg ccattcgttg tgcggtgtaa
    15781 ttttggagta gataaacaga aaaataatca ccatagccac gccaataaaa aaataggcac
    15841 taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt aaccaataat
    15901 atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc actctggctc
    15961 gccgagatcg ttaaaagatt cctcaccatt taacagtaaa tattcgtaag tatcttcggt
    16021 tacttgccgc tggtagccca tggtaaaatt atggacctcc catttggcat ggttttgatt
    16081 ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc tgttaaaaaa
    16141 tcgctgccag cgtttttcct gccagtttaa ggtcattgcc cccatcgttt tggcgttgat
    16201 cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga caaagagctt
    16261 gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca gaaattcatc
    16321 gcctgaagtg tagtaacgga aaatctggct accgcctccc agatcgatgc ggctgacagc
    16381 ggctaccgta aattcctcac ccggtagcgc aatcagcaat tcatcttcca gcaaacgaaa
    16441 cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac cgcgagcgat
    16501 agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat tttatcctta
    16561 aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg ttggcgttgc
    16621 agtcttgcta atacatcctg ggctctactt ttattgctac cgccaattcc ggcttccgcc
    16681 agcttttcgt caaggtcgcg accgtctgcg actttctcca actgtgcggc agcatccagg
    16741 cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc cgctgtcgaa
    16801 acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg ctgcatggct
    16861 tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg cgctgtcgct
    16921 gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac ttgctcttca
    16981 gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat caacgacgga
    17041 ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt gcgctcacgt
    17101 aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac gcgagatttt
    17161 ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat gcgcacgccc
    17221 tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag cgtaaataaa
    17281 ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc ttctgtaatt
    17341 tcagccagat ccaatgcgtt atctaccagc taggttatct caagcaggat atcttcaaga
    17401 gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg cacgctggag
    17461 attcctaccg atgacaacgg catcatcttc tgatt
//
LOCUS       DAJTNN010000077        17487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTNN010000077 DAJTNN010000000
VERSION     DAJTNN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.077"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..644)
                     /locus_tag="OO150_002263"
     CDS             complement(<1..644)
                     /locus_tag="OO150_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5516395.1"
                     /translation="MSLNFTVIVWLRLSIGGVYELAFCRSSLKSKKYFIILLALAAIA
                     GLGTHAAWSSNGLPRIDNKTLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKG
                     TQDARELGNAFSADIPDFDLYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAI
                     KDLQSKAPDKNIVIFTHNHCLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDG"
     gene            895..2034
                     /gene="arnB"
                     /locus_tag="OO150_002264"
     CDS             895..2034
                     /gene="arnB"
                     /locus_tag="OO150_002264"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5516396.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2038..3006
                     /gene="arnC"
                     /locus_tag="OO150_002265"
     CDS             2038..3006
                     /gene="arnC"
                     /locus_tag="OO150_002265"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5516397.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3006..4988
                     /gene="arnA"
                     /locus_tag="OO150_002266"
     CDS             3006..4988
                     /gene="arnA"
                     /locus_tag="OO150_002266"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5516398.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHATRQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4985..5875
                     /locus_tag="OO150_002267"
     CDS             4985..5875
                     /locus_tag="OO150_002267"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5516399.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5875..7527
                     /gene="arnT"
                     /locus_tag="OO150_002268"
     CDS             5875..7527
                     /gene="arnT"
                     /locus_tag="OO150_002268"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5516400.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            7524..7859
                     /gene="arnE"
                     /locus_tag="OO150_002269"
     CDS             7524..7859
                     /gene="arnE"
                     /locus_tag="OO150_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5516401.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7859..8245
                     /gene="arnF"
                     /locus_tag="OO150_002270"
     CDS             7859..8245
                     /gene="arnF"
                     /locus_tag="OO150_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5516402.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8239..8505)
                     /gene="pmrD"
                     /locus_tag="OO150_002271"
     CDS             complement(8239..8505)
                     /gene="pmrD"
                     /locus_tag="OO150_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5516403.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(8615..9970)
                     /gene="menE"
                     /locus_tag="OO150_002272"
     CDS             complement(8615..9970)
                     /gene="menE"
                     /locus_tag="OO150_002272"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5516404.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9967..10929)
                     /gene="menC"
                     /locus_tag="OO150_002273"
     CDS             complement(9967..10929)
                     /gene="menC"
                     /locus_tag="OO150_002273"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5516405.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELLQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10929..11786)
                     /gene="menB"
                     /locus_tag="OO150_002274"
     CDS             complement(10929..11786)
                     /gene="menB"
                     /locus_tag="OO150_002274"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5516406.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11801..12559)
                     /gene="menH"
                     /locus_tag="OO150_002275"
     CDS             complement(11801..12559)
                     /gene="menH"
                     /locus_tag="OO150_002275"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5516407.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(12556..14226)
                     /gene="menD"
                     /locus_tag="OO150_002276"
     CDS             complement(12556..14226)
                     /gene="menD"
                     /locus_tag="OO150_002276"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5516408.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14315..15610)
                     /gene="menF"
                     /locus_tag="OO150_002277"
     CDS             complement(14315..15610)
                     /gene="menF"
                     /locus_tag="OO150_002277"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5516409.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(15689..15994)
                     /gene="elaB"
                     /locus_tag="OO150_002278"
     CDS             complement(15689..15994)
                     /gene="elaB"
                     /locus_tag="OO150_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5516410.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16049..16510)
                     /locus_tag="OO150_002279"
     CDS             complement(16049..16510)
                     /locus_tag="OO150_002279"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5516411.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            16575..>17487
                     /gene="rbn"
                     /locus_tag="OO150_002280"
     CDS             16575..>17487
                     /gene="rbn"
                     /locus_tag="OO150_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5516412.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFN"
BASE COUNT         4111 a         4697 c         4438 g         4241 t
ORIGIN      
        1 tccccatcca gataaacttt gcctttttcc acatgcatga ctaaaccatc cagataatca
       61 ggtttaaatg tcgcatcacg cttattttta gcaatatatg tcaggcaatg attatgggtg
      121 aaaataacga tatttttatc aggcgctttg ctttgtaagt ccttaattgc actataaatc
      181 tcattaccgc actgaagaag tcgtttatct accgtcagct ttttacccgc ggaaaaccag
      241 gtcgccgact gaatggtccg gacggtatta ctggaataaa gatcgaaatc agggatgtca
      301 gcgctaaaag cgttgcccag ttcacgggca tcctgggtac ctttaaccgt aatacctgtt
      361 ttatctgaca agcattgatt ggttgaacga tcgcaacgtt cagcatggcg aaacaaaacg
      421 acaaccgggt gctgctgtgc cagtctggcc aatgttttat tgtcgatacg tggcaaacca
      481 ttgctactcc aggcggcatg agtacccagt ccggcaattg cagcgagcgc cagtaaaatg
      541 atgaaatatt tttttgactt caatgatgag cggcaaaaag ctaactcgta aactccacct
      601 atagacaagc gcaaccagac aattaccgtg aaattgagcg acatttctgg tgataattag
      661 cagttggtgt aatattaaaa atcctacgat gtcggcaaaa tgcctcaaaa ttttgccaaa
      721 tgcaaagcct aaataagaaa aaatataaaa atttcaatat ttacgtctaa tattagtttc
      781 ttaaggttaa gttaatattc tatccttaaa atttcgttcc aaatggcaaa atatacacaa
      841 cactctttat agcaaatata agtggacagg tattcaatgg cggaaggaaa agcaatgtca
      901 gaatttttgc ctttttcgcg accagcaatg ggcgtggagg aactcgctgc agttaaagaa
      961 gttctcgaat ccggttggat cacaaccggt ccgaagaatc aggcgcttga gcaagccttt
     1021 tgccagttga cgggaaatca gcatgccatc gcggtcagtt cagccaccgc cggaatgcat
     1081 atcacgctaa tggcgttgga aattggcaag ggcgatgaag tgattacgcc ttccctgacc
     1141 tgggtttcaa ccctcaatat gatttccttg ttgggtgcaa cgccggtaat ggtggatgtc
     1201 gaccgcgata cgctgatggt cacgcctgaa gcgatcgagt cagccattac gccacgcact
     1261 aaagccatca ttccggtgca ttatgccggt gcgccagcag atattgacgc cattcgcgcc
     1321 attggcgaac gttacggcat cgcagttatc gaagatgctg cccatgccgt cggtacgtat
     1381 tacaaagggc gacatattgg cgcaaaaggt accgctattt tttcatttca tgccattaaa
     1441 aatattacct gtgcggaagg tggcctgatt gtaactgata atgaaaacct tgcccgccag
     1501 ctacggatgc tgaaatttca cggcctgggt gtcgatgcct acgacagaca aacctggggc
     1561 cgagcaccgc aggctgaagt cttaacaccg ggctataagt acaatctgac cgatattaac
     1621 gccgcaattg ccctgacaca gttagcaaaa ttagagcacc tcaacacccg tcggcgcgaa
     1681 attgcccagc aatatcagca agcactggca gctctcccct ttcagccatt aagccttccc
     1741 gcctggccgc acgttcacgc ctggcatctg tttattattc gtgtcgatga acaacgttgt
     1801 ggtatcagtc gcgatgcgtt gatggaagcg ttaaaagaaa gaggcattgg taccgggtta
     1861 catttccgcg ccgctcacac acaaaaatat tatcgcgagc gttttcccac gctgtcgtta
     1921 ccgaataccg aatggaatag cgaacgcatc tgttctttgc cgctgttccc ggatatgact
     1981 accgccgatg ccgaccgtgt catcacagcc cttcagcagc tcgcaggaca ataagccatg
     2041 tttgaaatcc accctgttaa gaaagtctcg gtggttattc ccgtttataa cgagcaggaa
     2101 agcttaccgg aattaatcag gcgcaccacc acagcctgtg aatcgttggg gaaagagtat
     2161 gagatcctgc tgattgatga cggcagtagc gataattccg cgcatatgct ggtcgaagcc
     2221 tcacaagcgg agaacagcca tattgtgtct attttgctta accgcaatta cgggcaacat
     2281 tcagcgatta tggcgggatt cagtcacgtt actggcgact taattattac ccttgatgcc
     2341 gatctccaga atccgccaga agaaatcccc cgcctagtgg caaaagccga tgaaggttac
     2401 gacgtggtag ggactgtacg ccagaaccgc caggacagct ggtttcgtaa aacagcttcg
     2461 aagatgatta accggcttat tcagcgcacc accggcaaag cgatgggtga ctacggttgt
     2521 atgctgcgcg cctatcgccg tcatattgtc gatgcgatgt tgcactgcca tgaacgtagc
     2581 acctttatcc cgattctggc gaatatcttc gcccgccgtg ccattgaaat tccagtacat
     2641 catgccgagc gtgagtttgg tgaatccaaa tacagtttta tgcgcctgat taatttgatg
     2701 tacgacctgg tgacctgcct taccacaacg ccgctacgta tgctgagtct gctcggcagc
     2761 attattgcga ttggaggttt tagcattgcg gtgctgctgg tgattttacg cctgaccttc
     2821 ggaccacaat gggcggcaga aggcgtcttt atgctatttg ccgtgctgtt tacttttatt
     2881 ggcgctcagt ttatcggcat gggattactc ggtgaatata tcggcaggat ctacaccgat
     2941 gtccgcgccc gcccccgcta ttttgttcag caagttatcc gtccatccag caaggaaaat
     3001 gaataatgaa aaccgtcgtt tttgcctacc acgatatggg atgcctcggt attgaagctc
     3061 tgctggctgc cggttacgaa attagcgcca tttttaccca taccgataat cccggtgaaa
     3121 aagcctttta tggttcggtg gctcatctgg cggcggaaag aggcattccg gtttatgcgc
     3181 cggataacgt taatcatccg ctgtgggtgg aacgcattgc ccaactgtca ccagaggtga
     3241 ttttctcttt ttattatcgc catcttattt gcgacgagat tttgcagctc gctcccgcag
     3301 gtgcatttaa tctgcacggt tcgctgttac caaaatatcg tggtcgcgcg ccgctgaact
     3361 gggtgctggt gaacggtgaa acggaaacgg gcgttacatt gcaccgaatg gtaaaacgtg
     3421 ccgatgctgg ggccattgtg gcgcaactgc gcgttgccat tgcgccagac gatatcgcta
     3481 ttacgctgca tcataaattg tgccatgcca cgcgccagct actggaacag acattacccg
     3541 ccattaaaca cggtaatatt ctggaaatcg cccagcgcga aaacgaagcc acctgttttg
     3601 gtcgcagaac gccggatgac agtttccttg aatggcataa accggcatcc gtactgcaca
     3661 acatggtacg tgccgttgcc gatccgtggc cgggtgcctt cagctatgtt ggcaatcaga
     3721 aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag caaagcacag ccggggagcg
     3781 tgatttctat tgcaccgctg ttgattgcct gtggcgatgg cgcgctggaa atcgtcaccg
     3841 gacaggcggg cgacggcatt actatgcagg gctcgcaatt agcgcagacg ctgggcctgg
     3901 tgcaaggttc acgcttgaat agccagcctg cctgcaccgc ccgacgccgt acccgggtac
     3961 tcatcctcgg ggtgaatggc tttattggca accatctgac agaacgcctg ctgcgcgaag
     4021 atcattatga agtttacggt ctggatattg gcagcgatgc gataagccgt tttctgaatc
     4081 atccgcattt tcactttgtt gaaggcgata tcagtattca ttccgaatgg attgagtatc
     4141 atgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat agccacgccg attgaatata
     4201 cccgcaaccc gctgcgcgta tttgaactcg attttgaaga gaatctgcgc attatccgct
     4261 actgcgtgaa gtaccgtaag cgaatcatct tcccgtcaac ttcagaagtt tatgggatgt
     4321 gtagcgataa atacttcgat gaggaccatt ctaatttaat cgtcggcccg gtgaataaac
     4381 cacgctggat ttattcggta tcaaaacaat tacttgatcg ggtgatctgg gcctatggcg
     4441 aaaaagaggg tttacagttc accctcttcc gcccgtttaa ctggatggga ccacgactgg
     4501 ataaccttaa tgcggcacga atcggcagct cccgcgctat tacgcaactc attctcaatc
     4561 tggtagaagg ttcaccgatt aagctgattg atggcggaaa acaaaaacgc tgctttactg
     4621 atattcgcga tggtatcgag gcgttatacc gcattatcga aaatgcggga aatcgctgcg
     4681 acggtgaaat tatcaacatt ggcaatcctg agaacgaagc gagcattgag gaactgggcg
     4741 agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca tcatttccca ccgtttgcgg
     4801 gctttcgcgt tgtcgaaagt agcagctact acggcaaagg atatcaggac gtagagcatc
     4861 gtaaaccgag catccgcaat gcccaccgct gcctggactg ggagccgaaa attgatatgc
     4921 aggaaaccat cgacgaaacg ctggatttct tcctgcgcac cgttgatctt acggataaac
     4981 catcatgacc aaagtaggct tacgcattga tgtcgatacc tttcgtggca cccgtgaagg
     5041 cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt caggccagca tttttttcag
     5101 cgtcggcccg gacaatatgg gccgccatct ctggcgactg gtgaagccac agtttttgtg
     5161 gaagatgctg cgctcaaacg cggcatcgct ttatggctgg gatattttac tggcaggtac
     5221 ggcctggcca ggtaaagaga ttggtcatgc caatgccgat atcattcgtg aagcggctaa
     5281 acatcacgaa gtcggcctgc acgcctggga tcaccatgcc tggcaagccc gtagcggtaa
     5341 ctgggatcgg caaacaatga tcgacgatat tgcacgcggt cttcgcactc tggaagagat
     5401 tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg cgtgccgacc agaaggtgat
     5461 cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat tgtcgtgggg ccatgccgtt
     5521 ccgtccattg ctcgaatcag gaaaccctgg cactgcgcaa attccggtga ccttacccac
     5581 ctgggatgaa gtgattggtc gggatgtgaa aacagaagat tttaacggtt ggttactcaa
     5641 ccgcatcctg cgagataaag gcacgccggt ttataccatt catgcagaag ttgaaggctg
     5701 cgcttatcag cataattttg tggatctcct caaacgcgca gctcaggaag gcgtgacatt
     5761 ttgcccttta agcgaactgt tatcagagac gttgccgctc ggacaagttg ttcgcggaaa
     5821 tattgccgga cgtgaaggct ggctgggttg ccaacaaatt gcgggtagtc gctgatgaaa
     5881 tcggtacgtt accttatcgg cctcttcgcg tttattgcct gctattacct gttaccgatc
     5941 agcacgcgtc tgctctggca acccgatgaa acgcgttatg cggaaatcag tcgagaaatg
     6001 ctggcatccg gcgactggat tgtgccccat ctgttagggc tacgttattt cgaaaaaccc
     6061 attgccggat actggattaa cagcattggg caatggctat ttggcgcgaa taactttggt
     6121 gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg cgctggtgac ctggtttact
     6181 ctgcgcttat ggcgcgataa acgtctggct ctactcgcca cagtaattta tctctcattg
     6241 tttattgtct atgccatcgg cacttatgcc gtgctcgatc cgtttattgc attctggctg
     6301 gtggcgggaa tgtgcagctt ctggctggca atgcaggcac agacgtggaa aggcaaaagc
     6361 gcaggatttt tactgctggg aatcacctgc ggcatggggg tgatgaccaa aggttttctc
     6421 gcccttgccg tgccggtatt aagcgtgctg ccatgggtag cgacgcaaaa acgctggaaa
     6481 gatctcttta tttacggctg gctggcggtt atcagttgcg tactgacggt tctcccttgg
     6541 ggactggcga tagcgcagcg ggagcctgac ttctggcact attttttctg ggttgagcat
     6601 attcaacgct ttgcactgga tgatgcccaa catagagctc cgttctggta ctacgtgccg
     6661 gtcatcattg ccggtagcct gccgtggctg ggattactcc ccggtgcact gtacacaggc
     6721 tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga gctggacgat aatgccgctg
     6781 ctgtttttct ccgtcgctaa aggtaaattg cccacctata ttctttcctg ctttgcatct
     6841 ctggcaatgc tgatggcgca ttacgctttg ctggcagcaa aaaataatcc tctggcgctg
     6901 cggattaatg gctggattaa catcgctttt ggcgtcactg gcattattgc cacatttgtg
     6961 gtctccccgt ggggaccaat gaacacgccg gtgtggcaaa ccttcgagag ctataaagtc
     7021 ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg gctggtacac cttaacaaac
     7081 gtcgaaaaga cctggtcttt cgccgcgctt tgcccgctgg ggctggcgtt gctggtagga
     7141 ttttcaattc ctgacagagt catggaagga aaacatccgc aattttttgt cgagatgaca
     7201 caagaatcac ttcaaccaag ccgctatatt cttactgata gcgtcggtgt tgccgcaggt
     7261 ctggcatgga gcctgcaacg cgatgacatc atcatgtatc gccagacagg tgagttgaaa
     7321 tacggcctta attatccgga tgcgaaaggg agatttgtca gcggtgatga gttcgcaaac
     7381 tggcttaatc aacatcgtca ggaggggatt attacactcg tgctttcggt tgaccgcgat
     7441 gaagatatca acagtctcgc cattccgccc gcagatgcca tcgatcgtca ggagcgtctg
     7501 gtgctgattc agtatcgtcc caaatgatct ggctaacatt agtctttgcc agcttgctta
     7561 gcgttgccgg gcagttgtgt cagaaacagg caacctgctt tgtggcgata aacaaacggc
     7621 gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg tcttggtctt gccatggtgc
     7681 tctggctgct ggtcttgcag aacgtaccgg taggcattgc ctacccgatg ttaagtctga
     7741 attttgtctg ggtgacgctg gctgcagtaa aactgtggca cgaaccggta tcgccgcgtc
     7801 actggtgtgg ggtggcgttc attattggtg gcattgtgat cctcgggagt acggtgtaat
     7861 gggcctgata tggggattat tcagcgtcat tattgcctca gttgcgcaat taagcctggg
     7921 ttttgcggca agtcatctgc cgccgatgac gcacttatgg gattttatcg cggctctgtt
     7981 agcttttggc ctggatgccc ggatactgtt actcgggctg ctgggttatc tgctttccgt
     8041 tttttgttgg tataaaacgc tacataaact tgcgctaagc aaagcctatg ccttgctcag
     8101 tatgagttat gtgctggtat ggattgcttc aatggtactt cccggctggg aagggacttt
     8161 ttcgctgaaa gcactactgg gagtagcctg tattatgagc gggttgatgc tgattttcct
     8221 gcccacgaca aaacaacgtt actgagtttt ccctgccgct gtacactgcc gttcccactc
     8281 atcggggcta taactgcttg ccgagaggac tttcaccgta tgcagctttt cacgattaat
     8341 acaataaagc gcattctgca aaggcgagag taaatccccc actttcacgg cgaagtcact
     8401 cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg cagagcataa gtacatgtct
     8461 gttatcttgt ttattgcagc acgatttttt gaccagccat tccattgcat tatcctgttt
     8521 gctaagagtt ttcacatcaa tttacagatt aacagataaa ctctttatca acggcaatgg
     8581 aatggcagaa atgagaagtg aaacggaggg ttaattattg ctgacgttgc acccactctt
     8641 ttagcgcctg acgtgaaatt ttaataccgc cgtttttcag ctccggcggt aaagtcagcc
     8701 agcgcaccgg ttgttgaaaa cgagccagct tatctttcac ccattcgcca agatcaacgg
     8761 tttgctggtc atactccacc accgctaccg gccgatgacc aaactccttg tcggcaacgg
     8821 gaacgataaa cacctgcaaa accgcaggat gtgcagcaat aacacgctcg acttcttccg
     8881 gctgaatacc ctcaccgcca ctgaataaca gattgtctaa acgtccgaca atggtcagct
     8941 tgccattatg catctcaccg cgatcgcgcg tagcgtacca gccttcgtca ttaaccagtg
     9001 aaaccagttg cccgttacgc cagtaacctt ctgccatact ggcagcccgc agccacactt
     9061 cattattaac gattttcact tcccgacccg gcagcggcga accaacgtct gccaggccgt
     9121 cggcttcttt cgcacatacc gtggaggcaa actcggtcag accatagccg caaaagcaac
     9181 gaatcccctg ctcgcgcgcc tgttccgtca actcgaccgg gatagccgcg ccgccaagta
     9241 acaccgcttt cagggaaacg gaactgcggt taaccagcaa acgccagagt tgtgttggca
     9301 ccagtgaagc gtgagtacag cctgccagca tttgctccaa tggctgttta tcacgcaccg
     9361 tcatccgcgc accagcgtat aaccagcgcc acataattcc ctgaccggag acgtgaaaca
     9421 gcggtaaaga gagcaaccag tcatcgtgat cgccaaacgg aatcagcgat aacacacctt
     9481 gcgcactggc aagatgggcc tgataagtat ggacagcggc tttcggtaaa ccggtggagc
     9541 cggaggtcaa tgtcattgaa caaagacgtg ttggttgcca cagagctgaa tgtgtgcctt
     9601 caaccatctg aatatgcagt gaagttaacg ccgggaaagt attttctcct tccagcacca
     9661 gcgcatattg cagtgtaaga tttggcagta attcctcaag caacggttgc ggaagctgtg
     9721 gatttacggg cagcacccgc gccccgcatt gcagtaacgc cagccaggcg agcagcgttt
     9781 gcggcgtatt ccacgcccgc aacatcacgc cgctgccctc aaccacaccc tgcgccgcaa
     9841 agcccgtagc taactcatcg acgcgagcac aaagctcgcg ccagttgagt tgctcgtcat
     9901 taagacgtaa ggcgatggct tctccccgca cttgccgcca gtgacgccac ggccagtcag
     9961 agaagatcat aacaaccgct ccagtgcatc aacttccacg acaggcagcg tgctacccgg
    10021 ccagcgacgt acctgctgcg cctgcatcag atccagcgtg tccagccctg gaatggtgtc
    10081 cggcgttaac caggcggcaa tccgcgccag ttgcgttaag cctaagctcg actcaataga
    10141 agaactgatc accgccgtca gcctcaacgc gtgcgccgcc tgtacctgct cgcgtacttt
    10201 ttccagactg cccgtgagcg tgggtttgat aactaccgcg cgcacgccct cttcagccac
    10261 aaaggcaaaa tccggctcgc gcaggctttc atcccaggca atggcaatgc cggtttcacg
    10321 ggcaaacgct cgcgaatcat cgcgggtttt gcacggctct tcgagaaacg cgatgcggtg
    10381 gcgataatcc gggttaacat atttggcaaa ctgctgacct ttcagcggtg tccaggcgcg
    10441 atttgcgtca agacgcaaat gcagatccgg aattgcctcc agcaacagat taaccaccat
    10501 gccgtcgcgc acggcttcgt acaatcccac tttgaccttc gccactttct cgcctggcat
    10561 atcagcaagt ttgaggatca gatcgtcagg atcgccatta cacagtggtg ccgcacggta
    10621 attggccgcc tgcgggagcg tatctgccag ctccgccaat gcacagctta cgccaaaggc
    10681 aacggaaggc atctgcagta gctcgcaatc gcctgccagc cagttattta cccaggcaag
    10741 cagcacgctt tgcgcctctt cccaggtttc ctggctgaag cccggcagtg gggagatctc
    10801 cccccaccct tcgcgctcgc cctcacgcag gcaaacgtac agcccgtcgc gggtttttaa
    10861 ccgcctgtcg cgcagaacca cccccgcgtc catggggatc tgccagcggt atacctgcgc
    10921 gctacgcatt acggattccg tttaaatttg ctgaaatcag gctgacgttt ctggttgaaa
    10981 gcgttgcgac cttcctgacc ttcttccgtc atgtagaaca gcatggtggc gttgcccgcc
    11041 agctcctgca gccccgcctg cccgtcacag tcggcgttca gtgcagcttt caggcagcgc
    11101 agcgccatcg gactgttttg cagcatttcg cggcaccagc gaacggtctc tttttccaga
    11161 tccgccagcg gtaccacggt gttgacaagg cccatatcca gcgcctgttt cgcgtcgtac
    11221 tgacggcaca ggaaccagat ttcccgcgct tttttctgtc cgacgatgcg agccatgtag
    11281 gaagcgcccc agccgccgtc gaaggaaccg actttcgggc cagtctgacc gaagatggca
    11341 ttatctgccg cgatagtcag gtcgcacatc atgtgcagaa cgtgaccgcc gccgatggag
    11401 tagccagcca ccatcgcgac aaccggtttc ggacaggtac ggatctgacg ctggaagtcc
    11461 agcacgttca ggtgatgtac gccggaatca tctttatagc cgccgtaatc accacgcact
    11521 ttctggtcac cgccggagca gaacgcttta tcacctgcgc cagtcagaat gatcacgccg
    11581 acgttgtcgt cataacgcgc atctgccagt gcctggatca tctcttttac cgtcagagga
    11641 cggaaggcat tgcgcacctg cggacgatta atggtgattt ttgcgatacc atcggtggat
    11701 ttttcataac gaatgtcctc gaaaccttcg gagcagtcgt gccattcaac cggtgcgtaa
    11761 agcattgctt catcaggata aatcatattg tgtcctttag tcagaaacgc aagatctgcg
    11821 ccagacttgc gattacgcca gcgggatttt cccgatgcgc gttatgtccg gcgcgaggaa
    11881 tgacatggca gtcggcagcc agttccgccg ccagggcgcg gaatttgctg tcacgttcac
    11941 cacataaata ataaaacgca aatgtgcggg cgctaaggtt agcacgtaaa tcaggctgga
    12001 cggcgagaga agtcgcctcc agcatggcgg caagggttgc gccattattg ttgctgcgca
    12061 gcgccaccag ctcccggcgt tgatcgtcat tgagcgaggc aaaaacaggc tgttgatacc
    12121 agtcggcaaa taccgccgtt aacggttctg tgcgaaaacg ctgcgcccat tggcgatcgg
    12181 aacgctgacg ttccgcacgt tgttcagcat tttgcagccc cggatgcccg ccttcgacaa
    12241 taaccccaca aagccccgcc agcccctggc aagccgccat catcgccacc cgtccgccaa
    12301 gcgagtaccc caccagccag aagtcaagga tgttgtaact aaccaaggtt ttacgcagta
    12361 agtcggtgac atcatcaaat ccatcgacgc taatcgccgc cgaaccacca tgacctggga
    12421 gatcaacata caaccgtgag tagtcggcaa acgcctcgcc cacttcttgc cattcgtggc
    12481 aatcgccgga aaaaccgtgc aaaaacacca gccagggtaa acctggtttt ccgtgttttg
    12541 cctgcgcgtg caggatcata aatggcttac ctgcgccaga agttgctgga gcgtttgcgc
    12601 accatcggtg tcgttaacca ccatttcaat caccgtggtg gttggcgtgc gccaggcgtc
    12661 ggcaaatgcc gtttcaagtt cctgccagtt ttgcggacga tgatatttca gctcgaacat
    12721 cgcggcggcg tgctcaaaat ggacgttttg cggcatcaga tagaaacgct cacgctcgct
    12781 ttgcggcgtt ggcaacagcg agaaaatttg cccgccgttg ttgttcacca caattaatac
    12841 cagcggcgca gaaacctgac gcaataacgc cagcgcgttg agatcgtaaa gtgcggagag
    12901 atcgcccaca atcgccagcg tcggtttgcc gcttgcccgc tgaacgcctg cggcggtcga
    12961 aagcagtccg tcgataccgc tggcccctcg gttgctgtac accgggtaac ctgccggaag
    13021 ttgcgaaagc gcatcaatca gacgtaccac caggctgtta ccgacaaaca attgcccctg
    13081 ttcaggcaga tagtcgctga tgcgatgcgc cagttgcgct tcgccaaacg catcgcgacg
    13141 ggcaataacc gcctgcattg cctgttccgc caggcgcggg atttcaacgc accagggctg
    13201 gcgtttttct gccggatgca gctccagcca gtcggcaata ttggcaatta agcggcgtcc
    13261 acgatggtgc gccggatcaa gccgcccttc gatgtcatcc acaatccagt actcttcggg
    13321 ttcacagctt gcctgccatt gcaggagccg tttgcccgtc aggctgcttc ccagttgcac
    13381 cacaatttgc gcctgctgca gctcgctggt cgctttggca ttgcctaacc aaagatcggc
    13441 acacggcagc ggctgcccgg tttgtgacag cacatcgcca atcagcggcc agccaagagt
    13501 ttgcgcccac agggcaactt ttttgccctc ttccgcactc atgcgcccgg caaccaccac
    13561 gccgcgcttt tgtcgccaga agaaccagtc gcgctgtttt tcactttcca gactaggcgc
    13621 ttcacgcagc cacggtttgt cgtcctgcca ccagtcaccc agacgctgtt gccagctaag
    13681 cccggtatcg tccatttcgc catacagcgg ttcagcaaac gggcagttga tatggactcc
    13741 ccccgcatga agcgtaccga gagcgtggtc gatggtagaa accagccaac gtgcggggat
    13801 atcctgggtc gggcgcggca atgaaatact gtgcgtgggg tgagaggcga acattcccgg
    13861 ctggcgaatt gcctgattcg cgccgcagtc aattagctcc ggcgggcgat cggcggttaa
    13921 gagaatcagt ttttctccgg ttaacccggc ttcaatcagt gccggataga gatttgccac
    13981 cgccgtgccg gaggtcacaa tcaccgccac cggctgcttg ctgactttcg ccagccccag
    14041 cgccagatgc cccaacccac gctcatcgaa atgggtgtgg tgaatgaatg cggaattctc
    14101 cgccgccgct aacgttaacg gtgtagaacg cgagcctggg gcgatacaga tgtgtctgac
    14161 gccgtgacgc gttaatgctt ccagaatgac cgccgcccag cgtcggttaa atgcgcttac
    14221 tgacatgagt ttgtccggta tcaatattgc ggctaagtat aaggagctac aaaaatagat
    14281 tattgatatg aatcggtaat gatgcgactc attactattc catttgtaat aaagtacgca
    14341 accctgccgc tttgttgtcg atttcctgcc actcttgctc ggggtcggaa ccacggacaa
    14401 tgcccgcgcc agcatataat cgcacgacat tgccgctaat ttttgctgag cgcagggaaa
    14461 cgcagaattc gctttgttgt aatgagagat agcccgcaga accggcgtac cattctcggg
    14521 tgaacggttc gtgacgggcg ataaactgtc gcgccagatc gcgcggtaag ccagcaactg
    14581 ctgccgttgg ctgcaactga tgtaaacaga tcacatcatc cgctttgttg agtgaagtcc
    14641 agatacagcg gcgaagatgc tgcactttac gcagacgcag tacctgcggc ggtaaaacat
    14701 ccagcgtctg ggtatcggcc tgtaatcgtt ggcagatatc ttccaccacc agcatgttct
    14761 cgcgctggtt tttatcatcc gccatcagcc actcttctaa ctgctgcgcc tgcttatcat
    14821 caggatgatt tgctactgtt cccgccagcg cttcagtacg cagcgctttg tcacgccgcc
    14881 gccataaccg ttccggtgaa gagccaagaa aagcattttc gccatcaaag gccatgtaaa
    14941 aatggtagca attcagattc agtcgacgac tggcagccat catcgccgcc gcgttgaccg
    15001 gacttgcgaa atgcaggtca gttgcccgag cgagcaccac tttgtcgagc tcaccttcgg
    15061 cgatggtttt cgttgccagt tcgattaatt gcgtccagcc cgttttgtcc ggccagtgtt
    15121 gttctcgcgt ggtggttaaa tgtaacccag gcaagggctt gatactcacc agtgtggcga
    15181 taaattcttt tgcctgaatc gcatcgtgct gaagggagct ttcgctgaat agcgtcagcc
    15241 gcagcgtggc tttaccgcca cagcgtcgcc attccaggcg gggtaaaagt aaattgccct
    15301 gcgacgggtc aaaagcattc agcccccaaa tgcgtaagtc ggcgtgttcc gggtgctggc
    15361 gaagaaaacg ttgtgcctgg tccaacgacg taaaacgggt aatcgcgccc aggacggcag
    15421 cttcttcatc accattacgt tgttgccagt agaattgcgg gtacgtttgc tgactggcca
    15481 gccagctcaa ggcatcaaaa gcgtctttga gagggaaagg aatatcgata acccgaatgc
    15541 cgggtgtcgc cggaatctct tgcgacaaat ggcgcagtag attttccagc gccgtagtaa
    15601 gtgattgcac gcggacctct ccctgttaaa agcctcacat tatacggggt actacaaaaa
    15661 aaatgcagta ccccggtgta gggaggtttt aacggcgtgc cagcaacagt cctagtacca
    15721 gcccaacggc cgcgcccaca ccaattcctt gccaaggttt ttcgtggacg tagtcatcag
    15781 cacgataaac agcctgcttc gcccgatagt aataactgtc tgaaacctgg ctaacccgtt
    15841 ttttcacatc atccagcgct ttttctgcac gcgctttcag ctcaacatat ttctgatcag
    15901 cgggatcgcc agaggagcgg agcacctctt ccagtgtctc actaagcagc gtcaggtcgt
    15961 catcgatacg tgtatcacca aactgattag acattctcgt tctccatttg cgtaaaacct
    16021 gtcactaact atagacaagg gttgttgatt acgcctgaat tacctcgcgt gccatcccaa
    16081 tgtgtgggat accatcttct tcatagacct ccgtcacagg gataaaaccg aaactctggt
    16141 agaagttttg cagatgcgcc tgcgccccga ggtaaacagg cttatcaggc cagtgatgcg
    16201 tgcaggtgtc gagggtttta ctcatcagtt gctgccccac tttttcgcca cgcaacgctt
    16261 cactgacaat cacccgacca ataacgaccg gctcaagatc atcatcactt ttcagaatcc
    16321 tcgcatatgc caccagttca tcatttttcc aaccgagaat atggcgatta tcccccgtca
    16381 aatcatcacc atcgatatcc tgataagggc aattctgctc gacaacaaac accgcgcaac
    16441 gcagttgtaa taaggcataa agctgggaaa ccgataattc agagtgatgc agatcttgcc
    16501 attcaatcat aatgctctcc ttttagatgt ataccttata ctagccggaa tattttttga
    16561 aacgtgatga actcatggaa ttaatttttt taggtacttc agccggtgtg ccaacccgca
    16621 cgcgcaatgt cacggcaata ttgcttaacc tgcaacatcc gacccagagc ggactttggt
    16681 tgtttgactg cggtgaaggc acccagcatc agctactgca taccgccttt aaccctggaa
    16741 aactggacaa gatttttatc agtcaccttc atggcgatca tctttttggt ttacccggct
    16801 tgctgtgcag tcgttctatg tcaggcatta tccaaccctt aacgatttat ggtccgcaag
    16861 gtatccgtga atttgtggaa accgcgctgc ggattagcgg ctcatggacc gattatccgc
    16921 tggaaattgt cgaaattggc gctggcgaaa ttctcgatga tggcctgcgc aaagtaaccg
    16981 cttatccgct ggaacaccca ctggaatgtt atggctatcg tattgaagaa catgataaac
    17041 cgggtgcatt aaatgcccag gcattaaaag ctgctggcgt gccgcctggc ccactgtttc
    17101 aggaattaaa agcgggcaaa acaatcacgc tggaagatgg aaggcagatt aacggcgcag
    17161 attacttagc tgctccagtg ccaggtaaag cgctcgctat tttcggcgat accggcccct
    17221 gcgatgccgc acttgacctg gctaaaggtg tcgatgtcat ggtgcacgaa gcgacgctgg
    17281 atataaccat ggaagccaaa gccaatagtc gcggccatag ctctacacgc caggctgcga
    17341 cactagcccg tgaggctgga gtcggtaagc taatcattac ccacgtcagc tcgcgctatg
    17401 atgacaaagg ttgtcagcac ctgttacgtg aatgcaggtc aattttcccg gcgactgaac
    17461 tagcgaatga tttcaccgtg tttaacg
//
LOCUS       DAJTNN010000078        17053 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTNN010000078 DAJTNN010000000
VERSION     DAJTNN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.078"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            86..523
                     /locus_tag="OO150_002281"
     CDS             86..523
                     /locus_tag="OO150_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCR5516413.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLADILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDSYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     gene            657..1201
                     /gene="cobU"
                     /locus_tag="OO150_002282"
                     /pseudo
     CDS             657..1201
                     /gene="cobU"
                     /locus_tag="OO150_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            1198..1941
                     /gene="cobS"
                     /locus_tag="OO150_002283"
     CDS             1198..1941
                     /gene="cobS"
                     /locus_tag="OO150_002283"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5516414.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILALLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            1953..3032
                     /gene="cobT"
                     /locus_tag="OO150_002284"
     CDS             1953..3032
                     /gene="cobT"
                     /locus_tag="OO150_002284"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5516415.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGGAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            3094..4029
                     /gene="ldtA"
                     /locus_tag="OO150_002285"
     CDS             3094..4029
                     /gene="ldtA"
                     /locus_tag="OO150_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5516416.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYEFDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVPISSGSRQMF"
     gene            complement(4085..4160)
                     /locus_tag="OO150_002286"
     tRNA            complement(4085..4160)
                     /locus_tag="OO150_002286"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4125..4127),aa:Asn,seq:gtt)
     gene            4487..4858
                     /locus_tag="OO150_002287"
                     /pseudo
     CDS             4487..4858
                     /locus_tag="OO150_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4857..5291
                     /locus_tag="OO150_002288"
                     /pseudo
     CDS             4857..5291
                     /locus_tag="OO150_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
     gene            5634..6350
                     /locus_tag="OO150_002289"
     CDS             5634..6350
                     /locus_tag="OO150_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5516417.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(6806..6881)
                     /locus_tag="OO150_002290"
     tRNA            complement(6806..6881)
                     /locus_tag="OO150_002290"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6846..6848),aa:Asn,seq:gtt)
     gene            complement(6982..8625)
                     /gene="yeeO"
                     /locus_tag="OO150_002291"
     CDS             complement(6982..8625)
                     /gene="yeeO"
                     /locus_tag="OO150_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR5516418.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKVLALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            8630..8705
                     /locus_tag="OO150_002292"
     tRNA            8630..8705
                     /locus_tag="OO150_002292"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8663..8665,aa:Asn,seq:gtt)
     gene            8867..10129
                     /locus_tag="OO150_002293"
     CDS             8867..10129
                     /locus_tag="OO150_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5516419.1"
                     /translation="MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRF
                     SGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVA
                     LAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
                     ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGD
                     YQQGRQLTRLAVVLTLHLFIRSSELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRG
                     AKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSENTVNRALRLMGYD
                     TKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAM
                     MQWWSDYLDVSREGYVAPYIYARRHKAA"
     gene            complement(10841..11725)
                     /locus_tag="OO150_002294"
     CDS             complement(10841..11725)
                     /locus_tag="OO150_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HCR5516420.1"
                     /translation="MGILEQEMKRLAQQAGGSHKTVHDRIKLVQRFCERLVLAQNVQI
                     RRVEQLKARYIEGYVRERLAQGITKRSLQNEMAAVRCILKQAGRDRLAQSERLNNRSL
                     GLSGASRNGTKRAITPEHYRYVLETARAKDPGMAAALELSRLMGLRSQEVVQSAQSLR
                     TWKQSLERGEPRLTVVFGTKGGRPRETVILDAVAIRKALDNALVVAEDRHGRLIDKPD
                     LKSAMKYWHSQASRLGLTGTYSPHSLRYAWAQDAIRHYLAQGFSEKEALAMTAMDLGH
                     GDGRGRYVAQVYGRKDTD"
     gene            12284..12430
                     /locus_tag="OO150_002295"
     CDS             12284..12430
                     /locus_tag="OO150_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516421.1"
                     /translation="MYRIKTLASVAMFCDPVFAEVCTGKRIDRPDNRRFFQVVKRWAL
                     STTA"
     gene            complement(12590..12907)
                     /locus_tag="OO150_002296"
     CDS             complement(12590..12907)
                     /locus_tag="OO150_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5516422.1"
                     /translation="MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVP
                     VTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTIDMGARSGKRLERIPDAVVNEVL
                     ARL"
     gene            complement(12908..13165)
                     /locus_tag="OO150_002297"
     CDS             complement(12908..13165)
                     /locus_tag="OO150_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR5516423.1"
                     /translation="MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLII
                     EPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIL"
     gene            complement(13514..13720)
                     /locus_tag="OO150_002298"
     CDS             complement(13514..13720)
                     /locus_tag="OO150_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003842122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5516424.1"
                     /translation="MMLQDWHPADIIAGLKKRGTSLSALSRQAGLASSTLANALTRRW
                     PKGERLIAEALGIKPEQIWPSRYR"
     gene            complement(13717..14178)
                     /locus_tag="OO150_002299"
     CDS             complement(13717..14178)
                     /locus_tag="OO150_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516425.1"
                     /translation="MATIPTQTHPLLAVPFSAATDFTVLADHCENVAETLIESEDPVL
                     RLALCGRLAACLTLLQPTLNDPIPSHLTDSLTVDALPADMPRFVPDAELLCDYSLTLA
                     QLLIGQGMQPKVEQTITGLLCELVWYFAAEMKAPRWLRTADGVKFIDEVAA"
     gene            complement(14549..16153)
                     /locus_tag="OO150_002300"
     CDS             complement(14549..16153)
                     /locus_tag="OO150_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516426.1"
                     /translation="MITKELVDESNFISQLVDIGRFDESYQQSVAFLNKLERITIRND
                     NYFIVLANIAGALVDIGQMQNNKNASELGCNLMEDNKEAFISVLGECLYYYNYGNALS
                     NLVSITNPNDHTFKSIEELVSLKNIYWRSFKLSAEEQEEFQAELSVNLANSLRSQFRL
                     SESLRYYDLTNLKGLDIPQAWVNRSMSLMVLNLISSSFSIKLLKEVRAGYIKASVSKN
                     IPPQWESFYLERIAQTNEKIAEYAVDDETDEHDEALTQQEFEVLSPYRQFCLRNHLTL
                     SEHGLYCPCVGSATDDLVIASRGGVTGDFIIPMEMVLNRLKSEFSLARHLYFDYLHPQ
                     NTDYIKYESHFLELYNDEVLGIEIEKIRTAFRLCFGILDKIAVAICELYNLYPTTKKG
                     TPQKNIYFQNFWQLDVDNRRQLFEKVKSPGLLALYSIATDLNKDKGGELAFYKEWRNG
                     LEHKFLVVHKSDKPEDIYESYQLIEDILFIKESDFIHHFEHLLQITRSAIFSFAFMVR
                     QEGMKEKKEGIHYMTRELHAINYSAD"
     gene            complement(16166..16531)
                     /locus_tag="OO150_002301"
     CDS             complement(16166..16531)
                     /locus_tag="OO150_002301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516427.1"
                     /translation="MVSDKRADIIVSLPQIKIPIEIKRDYHRDVWTALNGQLDKLYTK
                     NPDAAGHGIYLVFWFGSARPNSLPHLAKNTSQPENASAMENMLNETVPVDKRDRLSAI
                     VIDVSCEGIPPVEAPKSSV"
     gene            complement(16931..>17053)
                     /locus_tag="OO150_002302"
     CDS             complement(16931..>17053)
                     /locus_tag="OO150_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5516428.1"
                     /translation="TDYIVGYYSALRPHEYNGGFPPNESENRYWKNSNSVASFC"
BASE COUNT         4492 a         4018 c         4094 g         4449 t
ORIGIN      
        1 gggctgcatt caaggtgtgg caatattgtt tcatggctta tcaaccagag cgccattaca
       61 ggaaattaag tggagggcga gaggcatgaa aacgataaaa cccgaatcga tagctctttt
      121 ttgcctctta tctggagatg tggatctggc gaaacatctg gctgatatat tgccaataac
      181 ctgttttgtt ggtgaggact ggcagcaaga tttccagcct cttgatggtg ggcctgtgca
      241 atcgttatca gattcgtata gcgattattc agtgctgcta ttgatagcag ctgaaagcga
      301 gatatccgta ccagaatggc tcgctcattc tgagggaacg acggtaattt tgattgcaga
      361 acacaatgcc cgtttgctga ctggtccggc tggtggggct gtagcattaa tccattatct
      421 gaatgaaatc tgtcattcca gagaaaccgc tgtcaatgac gcattttgtc ggacgttaac
      481 acgaggaagt ctggctattc cgcaatgcca tcttctttcg taatggattg agaatgctgg
      541 atttttaatt atattacgcg cagtattaat cgcgtaaatg ttttgcggtc agcaacggtg
      601 ggggatccat tgttctgttc aatgaatacc atcatacaat tgaatcagga gtcggtatga
      661 tgatttcggt gacggggggc atacggagcg ggaagagtcg ccacgcagag gcgcttattg
      721 gggactcttc acaggttctg tatacgccac ctcgcaaatc cttgatgatg agatggctgc
      781 acggatagaa catcatcggc aaggccgccc gacgcactgg cacacagtgg agcgctggca
      841 acatcttgat gaattaattc atgcagacat taacccgcat gaggctgtat tgctggaatg
      901 cgttaccacc atggtgacta atctgttgtt tgattatggc ggcgataaag accctgatga
      961 atgggattat caggcgatgg aacaggcgat taatgctgag attcagtcgt tgattgctgc
     1021 ctgccaacgt tgtcccgcaa aggttgtatt agtgactaac gaagtgggaa tggggattgt
     1081 gccggagagt cgtctggcac gacattttcg tgatattgcc gggcgggtaa atcagcagct
     1141 ggccgctgcg gcaaatgaag tatggttggt ggtttcgggt attggagtaa aaatcaaatg
     1201 agtaaattat tttgggcgat gctctctttt attacgcgtc tgcctgtacc gcgtcgctgg
     1261 tcccagggac tggatttcga gcattattct cgcggtatta ttacttttcc tttaattgga
     1321 ttattgcttg gcgcgattag cgggctggtc ttcatggtgc tgcaggcatg gtgtggcgta
     1381 ccactggcgg cactgtttag cgtacttgtg ctggcgctga tgaccggggg attccacctg
     1441 gacggcctcg ccgatacctg tgatggcgta ttttctgcac gtagccgcga tcgcatgttg
     1501 gaaatcatgc gtgatagccg tttaggcacc cacggcggtc tggcattgat ttttgttgta
     1561 ctggcaaaga ttctggtatt aagcgagttg gctctgcgtg gcgaaccgat ccttgcattg
     1621 ctggcggcag catgtgcggt cagccgcggg actgccgcat tattgatgta tcgtcatcgt
     1681 tatgcccggg aagaaggact tggcaatgtg ttcattggca aaattgatgg gcgacaaacc
     1741 tgcgtcacac tcggcttagc cgctattttt gctgccgtat tattgccggg tatgcatggt
     1801 gtggctgcta tggtggtgac gatggtggcg attttcatcc tcggtcagct tctcaaacgt
     1861 acgctgggcg ggcaaaccgg cgatacgctg ggagctgcta tagagcttgg tgaactggtc
     1921 ttcctgctgg cactgctata aagagacgtg acattcaaac acttgccgat ttactgaata
     1981 cgatccctgc tatcgatcct gccgctatgt cgcgtgcaca acggcatatc gacgggttac
     2041 tcaaacctgt tggtagcctg ggaaggctgg aggcgcttgc catacaactg gcaggaatgc
     2101 cggggttgaa tggcgttccg catgtgggca aaaaagcggt actggtcatg tgtgccgatc
     2161 acggcgtctg ggaggaaggg ggcgctattt ccccaaaaga agtgacagcc attcaggctg
     2221 aaaatatgac ccgcggaaca accggcgtgt gtgtgctggc agcacaagcg ggcgctaacg
     2281 tctacgtaat tgatgttggt attgatactg ctgagcctat ccccgggctt atcaacatgc
     2341 gtgttgctcg aggtagtggc aatattgctt cagctccggc aatgagtcgc catcaggcag
     2401 aaaagttgct tttggacgtc atatgttata cgcgggagct ggcaaaaaac ggtgttacgc
     2461 tgtttggtgt aggtgaactg gggatggcaa acacgacacc ggcagcggca atagtcagca
     2521 caatcactgg ccgggctcct gaagaagtgg ttgggattgg cgcaaacctg ccgacagata
     2581 aactggctaa taaaattgat gttgtgcgcc gggcgattac gttgaatcaa ccaaatccgc
     2641 aggatggcgt tgatgtcctg gcaaaagtgg gtggatttga tttggtcgga atggctggcg
     2701 tgatgttagg cgctgcgtcc tgcggtttac ccgtgttgct ggatggattt ctttcttatg
     2761 ctgctgcgct cgcagcctgt cagatgtctc ctgcaatcaa accgtatctc attccttctc
     2821 acctgtcggc agaaaaagga gcgcgtatag cgctgtcgca tttggggctg gagccttatc
     2881 tcaatatgga gatgcgttta ggtgagggga gtggcgcagc tctggcgatg cccatcatcg
     2941 aagctgcctg tgcgatatat aacaacatgg gcgaacttgc tgccagtaat attgttctac
     3001 cggggaatac gacttctgat ttgaacagtt aaaaacgaaa agtctgataa aagctatact
     3061 taacggatag ctttcgcgac ttaggaaagg gacatgatgc gtcgtgtaaa tattttttgc
     3121 tcatttgctc tgctttttgc cagccatact agcctggcgg taacttaccc attacctcca
     3181 gagggtagcc gtttagtggg gcagtcgtat actgtaactg ttcctgatca caatacccag
     3241 ccgctggaga cttttgccgc acaaaacggg caagggttaa gtaacatgct ggaagcgaac
     3301 ccgggtgctg atgttttctt gccgaagtcc ggctcgcaac tcaccattcc gcagcaactg
     3361 attttgcccg ccactgttcg taaagggatt gtggttaacg tcgctgagat gcgtctttat
     3421 tactacccac cagacagtaa tactgtggaa gtctttccta ttggtatcgg ccaggctggg
     3481 cgagaaaccc cgcgtaactg ggtgactacc gttgaacgta aacaagaagc tccaacctgg
     3541 acgccaacgc cgaacactcg gcgcgaatat gcgaaacgag gggagagttt gcccgcattt
     3601 gttcctgcgg gccccgataa tcccatgggg ctgtacgcga tttatattgg caggttgtat
     3661 gccatccatg gtaccaatgc caattttggt attgggctac gggtaagtca gggctgtatt
     3721 cgtctgcgca atgacgatat caaatatctg tttgataatg ttcctgttgg gacgcgtgtg
     3781 caaattatcg accagccagt aaaatacacc actgaaccag atggctcgaa ctggctggaa
     3841 gttcatgagc cgttgtcgcg caatcgtgca gaatatgagt ttgaccgaaa agtgccattg
     3901 ccggtaaccc catctttgcg ggcgtttatc agcgggcaag aagttgatgt aaatcgcgca
     3961 aatgctgcgt tgcaacgtcg atcgggaatg cctgtgccaa ttagttctgg ttcaagacag
     4021 atgttttaag agcgttggta attagaaagc aaaaagcctg ctagaaagca ggcttttttg
     4081 aatttggctc ctctgactgg actcgaacca gtgacatacg gattaacagt ccgccgttct
     4141 accgactgaa ctacagagga atcgtgtgaa cggggcgcat attaatggca gtgcatggtg
     4201 atgtcaaagg gtgaaatgca tttttttgtt tgtttgctgt caatttcaac aaagtggtga
     4261 gctttcaatc ttattgggta atgaaccatc gtggtgcata ccctcctttt atagggcagg
     4321 ggaacgcgac agctgattaa aggagcaaat gacgaatcta tcataatctc ttatttgaca
     4381 gttggttagc ttgtacatca acgccaaaat aaaactggca agcatcttgc aatctggttg
     4441 taagtaatgg cggcacttgg gccgattctt aaaaaccgga ggcaacatga acttcagacg
     4501 cctgaaatac ttcgtaaaaa ttgtagatat tggtagcctg acccaggctg ctgaagtatt
     4561 gcatatcgca caaccagcgc tcagccagca ggttgccaca ctggaaggtg agttaaatca
     4621 acaacttttg atccgtacaa agcggggcgt tacgccaaca gacgccggaa aaattctcta
     4681 tacccatgcg cgggccattt tacgtcagtg tgaacaggcc caactggcgg tgcataacgt
     4741 tggtcaggca ttatcggggc aagtctcgat tggctttgca ccaggaaccg ctgcgtcatc
     4801 catcaccatg cccttattac aggcggttcg cgctgaattt ccggagatcg ttatctatcc
     4861 cgagcattgc tgaagtgcaa cgtgcgcttt atgacgccac caagctggtg agcggcaggc
     4921 ccggtgagga agtcaaacag cggctacgta ctggtactgt ggtaaccaca gatgacagga
     4981 actgggaatt acgttactca gcttctgcac ttcgttttaa cttaagccgg gccgtagcaa
     5041 ttgatatgga aagtgcaacc attgccgcgc aaggatatcg tttccgcgtg ccatacggga
     5101 cactactgtg tgtttcagat aaaccgttgc atggcgagat taaacttccc ggccaggcta
     5161 accgttttta tgaaggcgct atttccgaac atctgcaaat tggcattcgg gcgatcgatt
     5221 tgctgcgcgc agaaggcgac cgactgcatt cgcgtaaatt acgaaccttt aatgagccgc
     5281 cgttccgata ataagttctg aaccttctta tcagaaatag atgtgaagga gtaagtaaga
     5341 cacctggcaa atagcctgca ataatcgtgg gctgtttgct tccttgggcg gatacgtgtt
     5401 ttattatcgt cttaatgatt tccacatatt aaaagcaagt atgctttcaa aacacaatta
     5461 taaaaaatcc cgccaacaat ataagttttt ataaaattaa atataagatt atggctttag
     5521 aatattttta tttctaatag acgagatttt tcctgttatg atataatatg ccgaattaac
     5581 acatgttaac gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac
     5641 gtaaatgggc caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt
     5701 atgcaaaatt cggtgtagaa atctatgctg ctgctaaaca aggtgaaccc gatccagaat
     5761 taaacacatc tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg
     5821 ttattgataa agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtgcagggac
     5881 gttatgaagg ctttggtcct aatggctcaa tgattatcgc cgagacattg acttcaaatg
     5941 ttaaccgtac gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag
     6001 cggcaggttc tgtcagctat atgtttgaca atactggtgt gattgtattt aaaggatcag
     6061 accctgacca tatttttgaa attttacttg aagctgaagt tgatgttcgt gatgtgactg
     6121 aagaagaagg taacattgtt atttataccg aacctactga ccttcataaa ggaatcgcgg
     6181 ctctaaaagc agctggaatc actgagttct caacaacaga attagaaatg attgctcaat
     6241 ctgaagttga gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg
     6301 aagatgacga cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa ttatatatta
     6361 aagaaatctg tctttatggc agatttcttt aatctcatat aatccttata aaaaatataa
     6421 tgttcaactc gtcatattgc ttataaaaaa tcctcgttcc cagagaaata atatttatta
     6481 taattccagt tcttcttttt ctgattacag aaggcaaagt gacaattacg catagtttcc
     6541 cgataaagac gcgacagcta catcccgcat aaggcatttt tctctttatc cttgtacggt
     6601 acttcatgga acagagtttt tgaccttgcg aatcgtgatg tctgttgggg aggggcaatt
     6661 tgctcactga agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaactcggg
     6721 cactttttta caaggtaacc gggtaaaaat aatttttatt ttttaactgt tttgagactc
     6781 atagagatgt ctcaaaacta aaatttggct cctctgactg gacttgaacc agtgacatac
     6841 ggattaacag tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa
     6901 tattagcgat gcccacccac aatgtcaaag cctgttattt aaatttgaaa tcgtttgctg
     6961 aaataatctg cattttgtcg tttattccga cacaactggc tttttttcac acttttgcgg
     7021 ttcggatcga gggtatttcc acagccaacg tccagtaacc attcgccagt aaaacagcac
     7081 ggcccgcaca gcccagtcgg caaacattcc catccagaca ccaaccacac cccagccaag
     7141 catgatgccc agcacataac cgacgacaac ccggcaaccc cacatgctca acatagaaac
     7201 ccacatggcg taacgggcat cgcgagcacc tttaaatcca gcgggtagcc cccatgaggc
     7261 ggaccaaata ggcataaata aagcatttag ccaaatcaga atcacaacga catgtttaac
     7321 ctgtggatcc tgggtgtaaa acgatgccat aaccccggca aagggagccg ttagccaggc
     7381 gatggccgtt aatccaagag tggaaagcca gaacacatgc cgcaactgaa tctctgcttg
     7441 cgctatctgc cctaccccca accttcggcc tgtaatgatc gtagaagcag agccgagcgc
     7501 acttccgggt aagttgataa gagccgcaat tgaaaacgcg ataaaatttc cggcaataac
     7561 actggtcccc atcccggcaa cgaacatttg ggttaataac cgaccactgg taaataacac
     7621 tgattcgaca ctcgcgggaa taccaatccc catgacttcc cagataatgc taaaattcag
     7681 cggtttaaaa tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac
     7741 ccacaaaatt gcaactgcgc caatataacg agaaatggtt aaacccagcc ctgccccgac
     7801 aaatcccagt cccggccagg agaaaaggcc gtaaatcaat atgccgctaa taataatatt
     7861 aagaatattc aggctaccgt taatcaatag cggtattttc gtattccctg caccacgaag
     7921 tgccccgcta ccaataagag tgatggcagc tgctggataa ctgagtaccg tcagctccag
     7981 ataagtcaac gccagtactt taacttctgt cgtggcatca cccgcgacga aatcaataat
     8041 ttgttcgcca aaatgatgaa taagcgttgc caacagtacg gcaaacaacg tcatgatcac
     8101 caatgactgc cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc
     8161 cacaacgaca gtagtaccaa gatcgatagc agcaaaaaaa gccataatga ccatattgaa
     8221 gctgtccgcc aatcccacgc cggccatcgc atcttttccc agccagctga ccagaaaagt
     8281 gctcagaacc cccatcaaca ggacacaggc attctccatg aagataggaa cagcaagcgg
     8341 ggttatctcg cgccagaaca acactttgta gctcttgcgt ttagcgtgcc agcgagtgcc
     8401 gtgaacaacc tggcgtaaag cagaggagat attcaaagcc gaccttaatt gcagaaagtg
     8461 aaaccacatt tcaaataatg agggagaatc agcaaagctg caaagatttt cgccaacaaa
     8521 ttgtctgcaa atgcaacaaa ctgttgatag aaacggcaaa cagttgggga atttaaaaat
     8581 cgggtttgac aaaagatttt tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt
     8641 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
     8701 agccaaattc cgattcttac gctatcttat tagtccttat ttgatattga ttcaataagt
     8761 tagcgtgaaa atctttccag atgtgtattc agttttccct ccgcatccgg ttaatttggt
     8821 ggtcagattt gggggcaggt tggttcgatt ctggagtgac ccccaaatgt ctttaaacga
     8881 ttcaaaaatc cgtaacttaa aaccctcttc aaaacccgta aaactgtctg attcgcacgg
     8941 tctgtatctg ctggtcaatc agggcggttc gcgtatctgg tatctcaagt atcgcttcag
     9001 tggtaaagag tcccgcgtca gtctgggcgc atatcctctg gtttcacttg ctgaagcaag
     9061 acaacgacgt gacgatatcc gtaagctact gacgcagaat attaaccccg cccaccagcg
     9121 catgtctgat aaagccgcag cgtcaccgga aaagtatttt aaagccgtgg cgcttgcatg
     9181 gcacaaaacc aacaaaaaat ggtcgcctga ttatgccgcg cgcatcctcg ccagcatgga
     9241 aaatcatatc tttccggcga ttggtcatct gcctgtcact acgctgaaaa cgcagaattt
     9301 cactgcgtta ctgcgggtta ttgaggataa aggttttctg gaagtcgcgt cccgaacccg
     9361 gcagcaactc tgcaacatca tgcgttacgc cgtacagcag ggattcaccg aaaacaacct
     9421 ggcgcagcat ctggaaggcg tcaccgcgcc gccggttaag aatcactatc ccgccctgcc
     9481 gctggagcgg ttgcccgaat tgtttgagcg cattggcgac tatcagcagg gacggcagct
     9541 tacgaggctg gcggtggtgt tgactctcca cctgtttatc cgttccagcg agctgcgctt
     9601 tgcccgctgg ggtgagatag atttcagaaa caaaatctgg accatccccg ccacaaggga
     9661 agcgattgag aaagtccgtt tctctggccg tggcgcaaaa atgcgtactc catatatagt
     9721 gccgctttct cgtcaggcta tcgccattct gaagcagata aaggaaatct ccggccatct
     9781 ggaactggtg ttccccggcg accataaccc gtataagccg atgagcgaaa acactgtcaa
     9841 ccgggcgttg cgtcttatgg gctatgatac caaaactgat gtctgtgggc atggattccg
     9901 gacaatggcg tgtagcgcat tagtggaaac tgaactctgg tcacgggata ccgtggagcg
     9961 gcagatgagt catcaggagc gcaacagcgt tcgggcagcg tatatccacc gggctgaaca
    10021 tctcgacgcc cgtacagcca tgatgcagtg gtggtcggat tatctggatg taagtcgcga
    10081 ggggtacgtt gcgccgtata tttatgcgcg gcggcataag gcggcctgag acagtggcta
    10141 tataatttgt taaaaatgaa aaagcagatc ctgattgaat ccggatctgc tttttacatt
    10201 aggcaatcgc tgccagacaa tctgcaccag aaacgccata actgcaccag ttcagtacca
    10261 ccgcttacct gcgccagtct gccgtcgcct gtctgtgtca ggactgcgcc agatcttatc
    10321 gaataaacat caaactgcgc cagtgttacg gccctttcag ccctttggcc ttttccggtc
    10381 tggcgctgtt acccttagcc gccccttacc ctttccggcc tttttccttt gctggcgcaa
    10441 tcgccagaaa aatcgtcaag ctgcaccaga agcctgccaa taggtcatac cgttcagaag
    10501 catcgttatt gagaggaaac cgtacaaaaa atttctacgt agaaattttt tcggtggata
    10561 acgatgaata aagagtattt tataatgtga tggctatcaa aaatcgacca tccaaaaata
    10621 attcattgat ttataatgaa taaccatgaa tttaatagtc gatgattatt gtttttatca
    10681 gtattgatag gaaagtatac aaaaaatttt ggccccaaaa ttttttgtac gaaaaccgat
    10741 gaataacgcg aaattaatag gaaaccatcc aaaattattt ggggcaaata attttgcccg
    10801 aaaaccgatg gataactcga tcttttcaaa gcaacggcta tcaatcagta tcctttcttc
    10861 cgtaaacctg cgccacatac cgcccgcgcc cgtcgccgtg tcccaaatcc atcgccgtca
    10921 ttgccagcgc ttctttctca ctgaatccct gcgccagata atgacgaatg gcatcctgcg
    10981 cccacgcata gcgcaacgag tgcggggaat atgtgccagt taacccgaga cgtgacgcct
    11041 ggctgtgcca gtatttcatc gcgcttttca ggtcgggttt atctatcagc ctgccgtggc
    11101 gatcttctgc cacaaccaac gcattatcca gcgcttttct gatagcgaca gcatccagaa
    11161 taacggtttc tcgcggacgc ccgcctttgg tgccgaacac cacggttaaa cggggctcac
    11221 cccgctccag cgattgcttc cacgttctta atgactgcgc actttgcacc acctcctgcg
    11281 aacgtaatcc catcagcctt gagagttcca gcgccgctgc catccccgga tcttttgcgc
    11341 gggcggtttc cagcacatag cggtaatgct ccggcgtgat ggcccgcttc gtaccgttac
    11401 gggatgcgcc agacaggccc agcgagcggt tattcagtcg ctcactctgc gccagcctgt
    11461 cacgcccggc ctgtttcaga atgcagcgca ccgccgccat ctcattttgc agggaacgct
    11521 tcgtgatacc ctgcgccagt cgttcacgaa catatccctc aatataccgc gccttaagct
    11581 gctccacccg gcggatctgc acattctgtg ccagaaccag acgctcacaa aaccgctgca
    11641 ccagcttaat acggtcgtga acggtcttat ggctgccgcc cgcctgttgt gccaatcgct
    11701 tcatttcctg ctccagaatc cccatacatt ccccctttca tgattgccgc aaactcatcc
    11761 ccaaaaactc aacactgata cagcgttccc cggcgcacag gccgatagcc cctgagtttt
    11821 gtcggggctg cgaacgatac ctgagcgccg gggcggcagc cgttgaggtg tggtggggct
    11881 tcggttgtgc tctcagagca gccgcccgct tgcgcgggtc atcccccgga tcgtccagat
    11941 ccgcctcggt atcggttcat attctcctta tgggtaaatg gcctaaaaag gcggtgtcag
    12001 aatccagcca tgagctggcg cagttgagtt ttctggtgca ggtcgaaagg gcttgcgtgc
    12061 gtcactttcc cgctttgcgc ggttaaagtg acgcacggat ctggtgcagc atctgagatt
    12121 tatcgtctgg tgcagtttta cggtcacgcg gcagcaaagc ctgacggcaa tgcaggccgc
    12181 agcccgtaag gtaaaaggcg ctgagggtgt cagcagatgc aggttagccg ggagaattcc
    12241 ggcgcggtgc agattcagac cagcaggctg gcgaagtaaa aggatgtatc gtattaaaac
    12301 tctggcctcc gtcgcgatgt tctgcgaccc ggtgtttgca gaggtctgca ccggtaaacg
    12361 gatcgaccgt cctgataaca ggcgcttctt tcaggttgtg aagcgttggg cgctgtctac
    12421 gacagcatag agcaaggggg agactacgcc tgtgccgctg gcgcggaaag gagaaactgt
    12481 ttttacctgc aaaaggattt tttaatcact tgttgttata aaaaatgagg gcgctgtatg
    12541 tgacagcgcc ctcgttcagg ttacataaaa aatatcagct cagaatagct tacagtcgag
    12601 ccagcacctc attcacgaca gcatcgggta tacgttccag ccgcttaccg ctccgagctc
    12661 ccatatcaat ggttctgggc tggtcgcagc gaataacgcc cgttgttttt gttcccgccc
    12721 cgtccagtga aacggtaaaa ccggcttcgc gggcaaagtt tccgccgctg gtgacaggga
    12781 caacaacagg caaccgggtg aacgtgttaa acgatgcctt cgatacgatg agtaccggac
    12841 gttttccact ttgttcatgg ccagtggtgg gatccagtga aacgagccag atttctcctc
    12901 tgtccatcta caggatctcc ttgcccaccg caggcgcatc aatccattcc cgaccctctt
    12961 cgcttatttc agcgtgcgga tcgcactgcg ccaacagttc ttcgagcgaa tagcggggac
    13021 gtttttgagg ttcaataatc aggcagccat tatcaatggt catgcccact tcgctgtccg
    13081 tcgacagccc tagcgttttc agcactgcgg ggggaaccgc cagcatgacg gatccgccaa
    13141 cctttttcag gcgagtggta tacatagagc acctccggat attatatttt aatataacat
    13201 ccatgataga cgatgcacaa ctattcatca gattcattgt ggtttcctta gtgtaataaa
    13261 gcgtgaagaa agccttaatc catcggaaga cagatccggc agttttcaaa ggcgacattg
    13321 ccggtaaaag ggcaaccgtc ctgatacatc aggcgcttct ttcaggtcat gaagcatttc
    13381 gtacaggcgg gagcttagtg cgtaacaaag aaggccgata ataggaaact gcttttgcag
    13441 ttcaaaggat tatgacggtt cggataaaca aaatgcggcg cagcttccgt atccgggctg
    13501 cgccgcagga acatcagcgg tagcgcgaag gccagatttg ctcaggcttg atgcctaacg
    13561 cttcggcaat taacctttcg cctttgggcc agcggcgggt aagcgcatta gccagcgtgg
    13621 aggacgccag acctgcctga cgcgaaagcg ctgacagcga agtgcctcgt tttttcagcc
    13681 cggcgataat gtcggcagga tgccagtcct gcagcatcat gcagccacct cgtcgataaa
    13741 cttcacgcca tcggcggtgc ggagccagcg cggggctttc atttcggcgg caaaatacca
    13801 gaccagttca cataataagc ctgttatcgt ctgctccact tttggctgca tcccctgccc
    13861 gataagaagt tgcgccagcg tcagtgagta atcacaaagt aattctgcgt cggggacaaa
    13921 tcggggcata tcggcaggta gagcatcaac ggtcaggctg tcagtcagat gagacgggat
    13981 ggggtcgttc agcgtgggtt gcagcaaagt gagacaggcg gcaagtcgcc cgcaaagcgc
    14041 cagccgcagc accggatctt cgctttcgat cagggtttcg gcgacgtttt cgcagtggtc
    14101 tgccagtacg gtaaaatctg tggcagcact gaagggaaca gcaagtaacg ggtgtgtctg
    14161 agtggggatc gtagccataa aggcagcctc cagtatgtgg tttgaaaacc accaccagag
    14221 gttccaatct catgggtggt ggactgaacg aggttggaac taccggccat actggaaccg
    14281 gcgcacctta cggtgccccc gcccagccca ccataatttt ggtgtagcta agcgcgcgac
    14341 ataaaaaaag acgcagacgc gtcacgtgtc gccagtatgg ttatatttcg gggttccaat
    14401 cccggcacct gattttgcag gtgcgggtaa aagataccgc tttcggttga aaacggcaag
    14461 gggaagtcgt ggaatagaga tcgcagattg ttataaatat acacaatagt taatgttaac
    14521 aaagacggca catatattaa ctttactctc aatctgctga atagtttatc gcatgtaatt
    14581 ctcgggtcat atagtggata ccctccttct tttctttcat cccctcttgc ctcaccataa
    14641 aagcaaacga aaaaattgct gaacgagtta tctgaagtaa atgctcaaaa tggtgaataa
    14701 aatcactttc tttgataaaa agaatatctt ctattagttg ataagattcg taaatgtcct
    14761 caggtttatc acttttatgt acaactaaaa acttatgctc taaaccattg cgccattctt
    14821 tatagaaagc caactcccca cccttatctt tattcaggtc ggtagcaata ctgtacagcg
    14881 ccagaagccc tggtgatttt accttttcaa ataattgtct tctgttatca acatctaact
    14941 gccagaaatt ctgaaaatag atattcttct gaggtgtacc tttttttgtt gttgggtaaa
    15001 gattataaag ttcacatatg gctaccgcaa ttttatcaag aataccaaag caaagacgaa
    15061 atgccgtcct gattttttca atttctattc ctaaaacttc atcattatac aattccagaa
    15121 aatggctttc gtacttaatg taatccgtat tttgtggatg aagataatca aaataaagat
    15181 ggcgtgcgag tgaaaactcc gatttaaggc gatttaatac catctccatg ggaatgataa
    15241 aatcacctgt tacaccgcct cgcgaagcaa tcacaagatc atctgtagca ctccctacac
    15301 atgggcaata caggccatgt tctgataaag tcagatgatt tctaagacaa aactgtcgat
    15361 atgggcttaa cacttcgaac tcttgttgtg taagtgcttc atcgtgttcg tcagtttcat
    15421 catcaacagc atattctgcg attttctcgt tagtctgcgc tatgcgttct aaatagaacg
    15481 attcccattg tggtgggata ttttttgata cagacgcttt aatatatcct gctctcactt
    15541 cttttagtaa cttgattgaa aatgaagatg aaataagatt gaggaccata agtgacatcg
    15601 aacggttgac ccaagcctgt ggtatatcta atcccttaag attggtaaga tcataatatc
    15661 tcaatgactc actgagccga aattgacttc ttaatgaatt agccaaatta acagatagtt
    15721 cagcctgaaa ttcctcctgc tcctcagcag atagcttaaa agaacgccag tagatatttt
    15781 ttaaggaaac caattcttct atcgatttga atgtatggtc attggggtta gtaatgctca
    15841 ccagattaga tagcgcatta ccatagttat aatagtaaag gcactctcct aacactgata
    15901 taaatgcttc tttgttatct tccatcaggt tgcaaccaag ctctgacgca tttttattat
    15961 tttgcatctg cccgatgtca acaagagcac ccgcaatgtt cgccaatact ataaagtagt
    16021 tatcgttcct gattgttatt ctttccagtt tgttcaggaa cgcaactgac tgttgataag
    16081 attcgtcaaa tcttccaata tcaaccagtt ggctaatgaa attactttca tcgacaagtt
    16141 cttttgttat caaaataaat tttccttata cagaggattt cggtgcttca acaggaggta
    16201 tgccctcaca ggagacatct ataactatcg ctgataagcg gtctctttta tcaaccggca
    16261 cagtttcatt aagcatattt tccattgcac tggcgttttc aggttgagat gtatttttcg
    16321 ccaaatgagg cagggaattc ggtcgagcag aaccaaacca aaaaaccaga tagataccgt
    16381 gaccagcagc atcggggttt ttagtataaa gcttgtccaa ttgcccatta agtgcagtcc
    16441 agacatcacg atgataatca cgctttatct cgataggtat ttttatctgc ggtagtgaaa
    16501 caatgatatc tgccctttta tcagaaacca tgtgtccttc tggttcacag cttatttttt
    16561 atggattaag ccggacacgg agtaaatcca gaaaaatatt acggcaactc tcttcaggtt
    16621 taggcgttag ggtacggcca tagctgtctt cattccagaa ttgcttataa atatcggtat
    16681 tttcattggc aattcggtta gaaatatccc gtatatgatc aagcaataat gagtaaagat
    16741 ccattatgtt ggcaggcgtt ttattcatca atgtacttac agtctgcttc cagtcggcat
    16801 gatggaactg tgattctcta taacgaattt tctgattgct ttgggcatgt gagagatgat
    16861 cccgataact ttcacattca ggcagcagta ataagcgctc aagtacttca ctagcatcgt
    16921 attgaagtgg tcaacaaaaa ctggccaccg agttagagtt tttccagtat cgattttccg
    16981 attcgtttgg ggggaaccca ccgttatatt cgtgcggtct tagtgcgctg taatatccaa
    17041 cgatatagtc cgt
//
LOCUS       DAJTNN010000079        17039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTNN010000079 DAJTNN010000000
VERSION     DAJTNN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.079"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1698
                     /gene="fhuE"
                     /locus_tag="OO150_002303"
     CDS             <1..1698
                     /gene="fhuE"
                     /locus_tag="OO150_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCR5516429.1"
                     /translation="TGLMTGTGNPSAAIDMVRKHATSREFKGDVSAEYGSWNKERYVA
                     DLQSPLTEDGKIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQ
                     RIDVNSPTWGGLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQA
                     TLNATHSEVEFDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDAL
                     DLFADGSYELFGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWS
                     PQSLAQDDTTHMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGL
                     VFDINDNWSTYASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSGWMNSRLTTTLAI
                     FRIEQDNVAQSTGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYI
                     AEDNEGNAVNPNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAE
                     QGSYALVDLFTRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(1758..3191)
                     /gene="ptsG"
                     /locus_tag="OO150_002304"
     CDS             complement(1758..3191)
                     /gene="ptsG"
                     /locus_tag="OO150_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5516430.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMHDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(3486..4283)
                     /locus_tag="OO150_002305"
     CDS             complement(3486..4283)
                     /locus_tag="OO150_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5516431.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(4294..5298)
                     /gene="holB"
                     /locus_tag="OO150_002306"
     CDS             complement(4294..5298)
                     /gene="holB"
                     /locus_tag="OO150_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5516432.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(5295..5936)
                     /locus_tag="OO150_002307"
     CDS             complement(5295..5936)
                     /locus_tag="OO150_002307"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5516433.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(5926..6948)
                     /gene="yceG"
                     /locus_tag="OO150_002308"
     CDS             complement(5926..6948)
                     /gene="yceG"
                     /locus_tag="OO150_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5516434.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(6951..7760)
                     /gene="pabC"
                     /locus_tag="OO150_002309"
     CDS             complement(6951..7760)
                     /gene="pabC"
                     /locus_tag="OO150_002309"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5516435.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(7880..9121)
                     /gene="fabF"
                     /locus_tag="OO150_002310"
     CDS             complement(7880..9121)
                     /gene="fabF"
                     /locus_tag="OO150_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5516436.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(9209..9445)
                     /gene="acpP"
                     /locus_tag="OO150_002311"
     CDS             complement(9209..9445)
                     /gene="acpP"
                     /locus_tag="OO150_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5516437.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(9656..10390)
                     /gene="fabG"
                     /locus_tag="OO150_002312"
     CDS             complement(9656..10390)
                     /gene="fabG"
                     /locus_tag="OO150_002312"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5516438.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(10403..11332)
                     /gene="fabD"
                     /locus_tag="OO150_002313"
     CDS             complement(10403..11332)
                     /gene="fabD"
                     /locus_tag="OO150_002313"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5516439.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(11348..12301)
                     /gene="fabH"
                     /locus_tag="OO150_002314"
     CDS             complement(11348..12301)
                     /gene="fabH"
                     /locus_tag="OO150_002314"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5516440.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVANQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(12369..13439)
                     /gene="plsX"
                     /locus_tag="OO150_002315"
     CDS             complement(12369..13439)
                     /gene="plsX"
                     /locus_tag="OO150_002315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5516441.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLRPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(13520..13693)
                     /gene="rpmF"
                     /locus_tag="OO150_002316"
     CDS             complement(13520..13693)
                     /gene="rpmF"
                     /locus_tag="OO150_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5516442.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(13745..14266)
                     /gene="yceD"
                     /locus_tag="OO150_002317"
     CDS             complement(13745..14266)
                     /gene="yceD"
                     /locus_tag="OO150_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5516443.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            14465..15049
                     /gene="yceF"
                     /locus_tag="OO150_002318"
     CDS             14465..15049
                     /gene="yceF"
                     /locus_tag="OO150_002318"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5516444.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASGYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLCEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(15161..16120)
                     /gene="rluC"
                     /locus_tag="OO150_002319"
     CDS             complement(15161..16120)
                     /gene="rluC"
                     /locus_tag="OO150_002319"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5516445.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            16406..>17039
                     /locus_tag="OO150_002320"
     CDS             16406..>17039
                     /locus_tag="OO150_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR5516446.1"
                     /translation="MKYTPVGVGIAKHVIQIHFINEHTDEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCK"
BASE COUNT         4248 a         4610 c         4136 g         4045 t
ORIGIN      
        1 acaggactca tgaccgggac gggtaatcca tctgcggcaa ttgatatggt tcgaaaacac
       61 gcgaccagtc gtgaatttaa aggcgatgtc tcggcggaat acggtagctg gaacaaagaa
      121 cggtatgtgg cggatttaca aagcccactc accgaagacg gtaaaatccg cgcgcgaatt
      181 gtcggcggct accagaataa cgactcatgg ctggaccgct acaacagtga aaagaccttc
      241 ttctccggca ttgtcgatgc tgatttaggc gatcttacga cgctttcagc cggttacgaa
      301 tatcagcgca ttgatgttaa tagccctacc tggggcggtt taccgcgctg gaatactgat
      361 ggcagcagca acagttacga tcgtgcacgc agtaccgcac ctgactgggc gtacaacgat
      421 aaagagatca acaaggtctt tatgaccctg aagcagcggt ttgctgatac ctggcaagcg
      481 acactgaatg ccacccactc tgaagtcgaa tttgacagca aaatgatgta tgtcgatgcc
      541 tatgtaaaca aagcggatgg tatgctggtt gggccataca gtaattatgg acctggcttt
      601 gattatgtcg gcggcaccgg ttggaacagt ggcaaacgta aagttgatgc gctggatttg
      661 ttcgctgacg gtagttatga attgtttggt cgtcagcaca atctaatgtt tggtggcagt
      721 tacagcaaac aaaacaatcg ttacttcagt tcatgggcca acatcttccc ggatgaaatt
      781 ggcagtttct acaactttaa tggcaatttc ccacaaaccg actggtcacc acagagcctg
      841 gcacaggacg ataccacaca tatgaaatcg ttatatgctg ccactcgagt cacccttgcc
      901 gatccgctgc atctgatcct cggcgcacgt tataccaact ggcgggttga tacgctgact
      961 tacagcatgg agaaaaacca caccacgcct tacgctggtc tggtgtttga catcaatgac
     1021 aactggtcga cctacgccag ctatacctct attttccagc cgcaaaatga tcgtgacagt
     1081 tcaggcaaat atctggctcc aatcaccggt aacaactacg agctgggtct gaaatcgggc
     1141 tggatgaata gccgtctgac caccacgtta gccatcttcc gtattgagca ggataatgtc
     1201 gctcagtcca ccggtacacc tatccccggc agcaacggcg aaaccgccta taaagcggtg
     1261 gatgggacag tcagtaaagg ggtggaattt gaactcaacg gcgcaattac cgacaactgg
     1321 cagctgacat ttggcgcaac gcgctatatt gcagaggata acgaaggaaa cgccgttaat
     1381 cctaatctgc cacgcaccac ggttaaaatg ttcaccagct atcggttgcc tgtcatgcca
     1441 gagttgacag tcggcggtgg tgtgaactgg caaaatcgcg tgtataccga caccgtgaca
     1501 ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc tggtggatct cttcacccgc
     1561 taccaggtga cgaaaaactt ctcgttacag gggaacgtca ataacctgtt cgacaaaacc
     1621 tacgatacca acatggaagg ttctatcgtc tacggcgcac cgcgtaattt cagcattacc
     1681 ggcacgtatc aattctgaat aacacctgta aaaaaggcag ccatctggct gccttagtct
     1741 ccccaacgtc ttacggatta gtggttacgg atgtactcat ccatctcggt tttcaggtta
     1801 tcggatttag taccgaaaat cgcctgaaca ccagaacccg caaccactac gcccgctgcg
     1861 cccagtttct tcaggccagc ctgatccact ttagacacat cagcaacgct gacgcgcaga
     1921 cgggtaatac atgcgtcgag gttagtaatg ttttctttac caccaaatgc agcaaccaga
     1981 gccggtgcca tttcgctggt acctgttgct tttgcatctt cagtcgcgtc ttcacgaccc
     2041 ggcgttttca gatccagtgc tttaatcagc acgcggaaga tggtgtagta aacaatcgca
     2101 taaccgatac cgacgatcgg gaacagccac agtttgctgc tgttaccaga cagaacgata
     2161 aagtcgatca gaccgtgcga gaacgacgta ccgtcatgca tccccagaag aatacagatt
     2221 gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca ggatcggcgc aacgaacatg
     2281 aaggagaact cgatcggctc ggtgataccg gtcaggaacg aggtcagcgc cgcggagatc
     2341 ataataccgc ccactttcgc gcggttttct ggtttagcag agtgccagat agcaattgcg
     2401 gcagctggca gaccgtacat tttgaacagg aagccaccag acagtttacc cgcagtcggg
     2461 tcacccgcca tataacgcgg aatgtcgccg tggaaaacct gacctgctgc gttggtgtat
     2521 tcaccaatct gcatctggaa aggtacgttc cagatgtggt gcagaccaaa cggtaccagg
     2581 caacgttcga tgaaaccgta aatgccaaac gcaactaccg ggttctggta agcagcccac
     2641 tgagagaagg tctggattgc agaaccaatc ggcggccaaa tgaaggacag cacaacgcca
     2701 gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc gtttaccggc aaagaagcca
     2761 agatactcag gcagcttaat acggtagaaa cggttaaaca tgtacgctgc gatcgcaccg
     2821 gagataatcc ctccgagtac gccagtatcc gctaggtgtt tagaggcgat ttcttcagca
     2881 ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt taaccatgat gccataggca
     2941 acaactgcgg ccagcgcgga tacgccatcg ttattggtaa agccgagggc gacaccgatc
     3001 gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg cttctgccat aacatgcgat
     3061 acaacggcgg gcagccagct gaaattcgcg gaaccgacgc ccagcagaat acctgcgata
     3121 ggtagtacgg ataccggcag catcagcgat ttaccgacct tttgcaggtt agcaaatgca
     3181 ttcttaaaca taattgagag tgctcctgag tatgggtgct ttttttacgt tctcacgcgt
     3241 ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag cgccctttat ttattacaca
     3301 gagtaaaata attcagtgcc aatttgtttg acggctatca cgtttcaact tcagatcatt
     3361 ggagaagttg cacattttga caaaacatat gtcacaatat ttcaaacgcg gttattcacc
     3421 gcccaatttt cggcggtgaa ctttactcgt tttagccatt aattacgagc tttaaaaaaa
     3481 ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt gaaacagacg ggcgaagtta
     3541 tcggtggtta cctgcgccag ttcttcaacg gcaacacctt tcaacacagc catgtattct
     3601 gcaacgtcac gaaccatcgc aggttgattc tcttttcctc gatgcggtac cggcgcaagg
     3661 taaggtgagt cagtttccac cagtaatcga tccaggggga cataacgcgc agcatcgcgc
     3721 agttgctccg cattacggaa ggtcacaatg ccggaaaagg agatgtaaaa tccgagatcc
     3781 agtaatttac ccgccgtttc tctgtcctct gtaaaacagt gtagtacgcc accgcaatcc
     3841 gtcacttttt cttcgcgcag aattgccagc gtatcggcac gggcgtcacg ggtatggacg
     3901 ataaccggct tgttcagttc acgaccaatc tggatatgat ggatgaagga ctcttgctga
     3961 cgtactttag tttccggtgt gtaataataa tccagcccgg tttcacccag cgctacaaca
     4021 ccctcttctg ccgccagacg gcgtaaatct tctacatcgt agggatcatt ctggttgagt
     4081 ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc ctaccagatc ccgcatatgt
     4141 aaataacccg gtaatgttgt ggcgactgcc agacaaaatt tcacatcgcg tgcggcggct
     4201 ttcgccagaa cgtcatccac gtccttatgc aaagattcat aatccagacc atcgagatgg
     4261 cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga tgaggaaccg gtagcacaac
     4321 gcccggttgc aggtaatgct caatacgcag taaaagatcg gtgatgagaa gctcgcggtt
     4381 gatgcctgtg acagacatta actgttcacg aatgtggcaa acatccccca gtatagcctg
     4441 caggcgcgag ggagaaagat ggtttgccag ttcggcgacc aggcccggca catcaacatt
     4501 ggtcacctgt gcagcgccat gatggcgttt tagcgcatcc atcagcaacg ttgccagcca
     4561 gtgtaaacgc gccggagctt gttcatgatt aagggccgcc agcagcgaat accagtcgcc
     4621 cgatggtacg ctatatgcca acgcctgaca caatgtttca cgagcctgcc agttatctcc
     4681 ctgaaacaac gccagtgccg cgctaggcga accggcactt aagcgcaatg cggcaagtaa
     4741 tgcatcctgt gacattgtca cttcgcgtga aagccaggtc acggcgtact gttccggcgg
     4801 cggcgcaagg taatgtaacc gacaacgact acgtaatgtt gccagtaaac gttcaggctc
     4861 gcgggtagcc aggaaaaacc aggtttctgc tggtggctct tcaagcgttt tcagcaatgc
     4921 gttagccgcg gcgtcggtca gtaaggcagc atcggttacc cagacaactt tcgcaccacc
     4981 taggcgtgcg tgctcattca gcttttcggt gacctcacgt accgcatcaa tgcccagcgt
     5041 atttttccct ttttcgggag ccagggtgta gtaatcggga tgcgtgccag cctgcatcaa
     5101 ctgacatcca cgacagtgac cgcaactttt gtggccctgc ggttgttggc agagtaaata
     5161 acggctcagg gcgtagatta aagcatcatc gcccatgccc ggtaacgcct gaatgagtag
     5221 cgcatggtga cctcttccgg cctgatagct ggctaccagt ttttcgaaat caggtcgtaa
     5281 ccatggatac catctcatgc gtccaactcc ttcacccagt gggtcacggt agtgcggatt
     5341 gcatccatca cggcctccag cggctgggtg gcatcaatgg tatgaatact tttatcttgt
     5401 gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga aatcgaaaga ctcttgctca
     5461 atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc caacttccgg ggtaacatcg
     5521 agatagagcg ttaagtcagg gcgaaaatcc ccgagaacag catcacgcag tgttgccagc
     5581 atatgttggt caataccacg tcctccgccc tgatacgcct gagtggagag atcgtggcga
     5641 tcgccaatca cccaggtgcc gttagccaga gctggtttga tgaccgtttc taccagttga
     5701 acgcgcgcgg cataaaacat cagaacttcg gctttatcgg taatgacttc atcgcctacc
     5761 gatttgatat ccagcaccag gcttcttaac ttttcggcaa gttgcgtacc gccaggttcc
     5821 cgagtgaaaa ccatgtcgcg gatacccagt tgctcaagcg tctcaaccac cacattacgc
     5881 gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga tatacttact gcgcattttt
     5941 ttccttaagc actttcagat aatcctgcac agacttgtta tggctggcaa gattggtatt
     6001 aaacgtgtga ccacctttac catcggccac aaaatagaga tacggcgttt ttgccggatg
     6061 cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc gcacctggcg gcaaaccggt
     6121 aatggtatag gtgttatacg ctgtcggcgt ttccaggtct gcacgagaaa gtttgccatt
     6181 ataacgctct cccatcccgt aaatcacggt tgggtcggtc tgcaggcgca taccaatgcg
     6241 taaacggttg ataaataccg aggcaacctg atcgcgttca ctggcaacgg cggtttcttt
     6301 ttcgataatt gatgccatcg tcaccagctg gtttttatct ttataaggca ggccgtccgc
     6361 acgcccttcc caggcgctat cgaccgcttt caccattttc ttgtgcgctc gcttgagtaa
     6421 cgcgacatcg gtggtattgg cggtatacat ccaggtgtct ggccagaacc aaccttcaat
     6481 ccactccggg ttttccagtt caagtgcctg cgctacggtg gcgtacttat cgtcactcag
     6541 cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg tagtcgctca gacgcatccc
     6601 ttctaccagt cgcagcggga actgtgcttc tttaccgctt tccagcaatt tcagcatctc
     6661 gcgcacggtc atctgcggtg taaagcggta agtcccggct ttaaagtgag aaagatccgg
     6721 ttcgatacgc agcaaccatt gaaaaacccg tgggcgattg atgatcttat cggcataaag
     6781 ctgttcacca agcgccagac gtccggtccc tggcttcagg gtaaatatcg tctcttcttt
     6841 gataagcaat ttgctgtcgg caagatggcg aaccttccag acgcccacac cagcggcgat
     6901 acccagtacc accagcaata acaagataat caataacact tttttcatga ctaattcggg
     6961 cgctcacaaa gtggggctaa atattcatat aacgttgctg acgaaaagga gacatcgcca
     7021 caggcacgta cgggcatcac tggcattaac gcattacaaa taaccatctc atctgcctgc
     7081 aacgcctctt ccagagaggc ttgcacttcg acaagctgat aagaggattg tgccagcaaa
     7141 cggatacaga attgtcgcat aatgccgtta acacctgcct gatccagtcg cggcgtataa
     7201 actacattgc ccttccgcca gaacaaatta gccgcacagc attccgtaac ccacccttcg
     7261 ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa gatgagagcg gatcaatact
     7321 tgctcaagcc gattaagatg tttaatacct gcaagatggg gattgcgccc cagccgcacc
     7381 gggcttagcg ccaacgtcat cccttcgtta cgcaaacggt cgtaatgtgc aggataaacc
     7441 gtaacggaga gaatccgcgt tgccggtcca ctgttcaatg tgctgtaccc tcgcccgcca
     7501 ctaccgcgac tgatcacgac tttcagcaca ccattttgct gttctgctgc cagcgttttc
     7561 atctcttgtt caagctgagg ccagaagtca cagaaaatca tcaaccgctg acaagcatcc
     7621 tgtagtcgct ggatatgcgc cgataacaaa ctgactttac cgtcgataac tctggcggtg
     7681 gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta ctgccagcga ttcctgctta
     7741 tgaccgttaa ttaagaacat accggctcct tatcagagaa ttgttagtgt ggcagcatgc
     7801 tcactacgga acaagtcgga ataaaagcta agaaaaaagg cccgcaagcg gaccttttat
     7861 atgggtggga aatgacaact tagatctttt taaagatcaa agaaccatta gtgccaccga
     7921 agccgaagga gttacacagc gtgtattcca ttccgctgac ctgacgtgct tcatgcggta
     7981 cgaaatccag atcgcaacct tcatccaggt tatccaggtt gatggttggc ggaacagcct
     8041 gatcgcgcag cgccaggatg gagtagatag actctactgc ccccgccgca cctaacaggt
     8101 gaccggtcat agatttcgtg gagcttacca acacacggct tgcagcttca ccgaagatgg
     8161 ctttcaccgc ctgcgcttca gctttatcgc cagccggcgt agaagtaccg tgcgcgttaa
     8221 cgtagccaat ctgactcgct tcaatgcctg catcacgcag agcatttgcc attgccagag
     8281 ctgcgcctgc gccattttct ggcggtgacg tcatatgata agcatcgctg ctcataccaa
     8341 agccgacgag ttcagcgtaa attttcgcac cgcgtttttt cgcgtgttcg tactcttcta
     8401 gtaccagcat accggcacca tcgcccagta cgaagccatc acgctcttta tcccacgggc
     8461 ggctcgccgc ttgcgggtta tcattgcggg tagataatgc acgtgccgcg ccaaaaccac
     8521 caacgcccag cggcgtactg gctttctctg cgccacctgc aaccatcacg tcagcatcgc
     8581 catacgcgat aatacgcgca gcatgaccaa tgttgtgcac gccggaagta caggcggtcg
     8641 cgatagagat gcttgggcca cgcaggccat acatgatagt cagatgacct gccaccatgt
     8701 tcacaatcgt tgacggaacg aagaatgggc tgatcttacg tggaccaccg ttcatcagag
     8761 atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc ggagccaatt gcggcaccaa
     8821 tgcgggttgc gttctcttcc gttatttcaa ggccagaatc ctgcatggcc tgaacgccag
     8881 cgacaattcc atattgaatg aaggcatcca tcttgcgctg ttctttgcgc gagataatgt
     8941 cctcacagtt aaaatccttt actaagccag caaatttcgt tgcataggcg ctagtatcga
     9001 aatggtcgat taggctgatg ccactctgac cggcaagcag agctttccag gtagactcta
     9061 cggtattgcc gacaggagac aacatgccca gtccggtcac aactacacga cgcttagaca
     9121 cgtttgtcct ccagggaggg aaaaaatgat tctagtggga caaaaagata aaactcaggc
     9181 ggtcgaacga ccgcctggag atgttcactt acgcctggtg gccgttgatg taatcaatgg
     9241 cagcctgaac ggtggtgatt ttctcagctt cttcgtccgg aatctcagta tcaaactctt
     9301 cttccagagc cattaccagc tcaacggtgt caagagaatc cgcgcccagg tcttcaacga
     9361 aagaagcatt gttggtaact tcttcctgct taacgcccag ctgttcgccg ataattttct
     9421 taacgcgttc ttcgatagtg ctcatactct taaatttcct atcaaaactc gctttcgcga
     9481 tggttttcgt agtgtataaa atgttgaaaa atttgcaact aaatcccggc aggtcttacc
     9541 acgattttac gttattttga ggccttaccc cccaataacg caaatcattt tgcactaatt
     9601 attactcata accacgcaga ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga
     9661 ccatgtacat cccgccgttc acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg
     9721 atgccaggaa tgcaaccgcg ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa
     9781 cctgcgccag gatacccgca cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa
     9841 taaagcccgg agcaacaacg tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg
     9901 atttactgaa gccgatcaag cccgctttcg ccgcagcgta gttggcctga ccgccatttc
     9961 ccatggtacc aaccacagaa ccgatagtga taatacgacc atgacgcttt ttcatcatag
    10021 cgcgcattac cgcttttgac agacggaaaa cagatgaaag gttggtttcg ataatatcgt
    10081 tccactcttc atctttcatt cgcattaaca ggttatcacg agtgataccg gcattattga
    10141 ccaggatatc cacttcacca aattctgcgc gaattttttc cagaacagat tcgatagatg
    10201 ccgggtcggt cacattcaac atcagacctt tgccgttagc acctaaataa tcactgatcg
    10261 cctgagcgcc attttcactg gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg
    10321 tttcagcaat tgcgcggcca attccgcggc ttgcaccggt taccagtgcg atttttcctt
    10381 caaaattcat gattttcctc ttttaaagct cgagcgccgc tgccatcgct gaaggttcgt
    10441 tcagcgccga ggcggtcagg gtgtcgacaa tgcgtttcgt caggccagtt agcactttgc
    10501 ccgggccgac ttcatagaga tgttctacgc cttgcgctgc catgtactca acagacttcg
    10561 tccactgaac cgggttatac aactgacgta ccagtgcgtc acgaatggca tcaccattgg
    10621 tttcgcattt cacatcaacg ttattcacaa caggaactgt tggtgcgtta aaggtgattt
    10681 tcgctaattc tactgccagt ttgtcggctg ctggtttcat cagcgcacag tgagacggta
    10741 cgctcactgg taacggcagc gcgcgttttg cgcccgcagc tttacaggca gcgccagcac
    10801 gctcaaccgc ctctttatga ccggcaataa ccacctgtcc cggagagtta aagtttaccg
    10861 gagaaacgac ctgaccttct gcagcttctt cacacgcttt cgcaatagac gcatcatcca
    10921 gaccgatgat tgccgccata gcgcccgtgc cttctggtac ggcttcttgc atgaacttgc
    10981 cgcgcatctc aaccagacgc accgcatcag cgaaatcaat cacgccagcg caaaccaacg
    11041 cggagtattc ccccaggctg tgaccggcca tcattgccgg tgctttaccg ccctgctgct
    11101 gccatacgcg atacagcgca acagatgcag tcaacagcgc tggctgggtt tgccaggttt
    11161 tattcagttc ttcagctggc ccctgctggg tcagcgccca caggtcgtag cccagcgccg
    11221 cagaagcttc agcaaacgtt tcttcgacaa ttggatagct cgccgccata tcagccagca
    11281 ttccaacggt ttgagaaccc tgtccaggga acacaaatgc aaattgcgtc atgttttaat
    11341 ccttatccta gaaacgaacc agcgcggagc cccaggtgaa tccaccgcca aaggcttcaa
    11401 gcagaaccaa ctgccccggc ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg
    11461 ggacagaggc cgcagaggta ttaccgtggc gatccaacgt caccacgaca ttgtccatag
    11521 acataccgag ttttttcgcc gttgcactga taatacgcag gttagcctga tgcggaacca
    11581 gccagtccag ttgagaacgg tcaagattat tcgccgccag cgtctcatca acgatgtgcg
    11641 ccagttccgt taccgcaacc ttgaagactt cgttgcccgc catcgtcaga tgaattgaat
    11701 tctctggatt cacgcggtcg gcgtttggca gcgtcagcaa ttcaccataa ctaccgtcgg
    11761 catgcagatg ggtggagatg attcccggct cttcagaggc agccagcacc gcagcgcccg
    11821 cgccatcgcc aaaaataata atagtcccac gatctgttgg atcgcaggtg cgcgccagta
    11881 catcggaacc gacgaccaga gcatacttca ccgccccaga tttcacgtat tgattggcta
    11941 cgcttaatgc ataggtgaaa cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt
    12001 taatgcccaa catgctttga atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag
    12061 tcgttgccac aacgatcagg ccaatctggt ctttctcaat gcccgccatc tcaattgcgc
    12121 gtgtcgccgc ttcaaagccc atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt
    12181 cgcggatacc ggtacgagtg acaatccact cgtcagaggt gtccaccatt ttttccaaat
    12241 cggcgtttgt ccgcacttgt tcgggcagat agctgccagt accaataatc ttcgtataca
    12301 tgtacgctca gtcacttttc ggttatatac cgtcacttgc aaactgcgag ttcgctggca
    12361 gcgtcctgct accgcagagt tccgcttttg ccaccgtcca gcagctcaaa accagctggg
    12421 tatacagatt ccaggcgagc ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact
    12481 gcctgttcaa tcgcgaccgc aaaagctcgc tgattggctg caccatgact ttttatcacc
    12541 gtgccgcgca atcctaacag acaggcgccg ttatactggt cggggttgag gtgactgaat
    12601 cgcctcgtca ggctcttttg tagccaacgc tttaataaca gtagccacca cgaccgtttt
    12661 ttcccttcac cctgagattt cagcagagaa aggaacatcc tgacaacacc ttccatcgtc
    12721 tttaacgtga catttcctgt aaagccgtca caaaccagca catctgtctt gccagttaac
    12781 aactcattgg cttcaagata gccgatataa ttgatagaag ggattgtttt aagcaccgct
    12841 gaggcatccc gaatactgtc gagacccttt acttcttctt caccaatatt gagcaacgcc
    12901 acgcgaggat tgggaatttc caccacctct tcagccagaa ctgagcccat aatggcaaat
    12961 tgcaccaaca ttgtgctgtc acaatcgacg ttggccccta aatcgaggac caccgttttg
    13021 cccttttgct gatgtggtaa taccgtcacc agcgccggac gctcaatccc ctccaggggc
    13081 ctgagtaata attttgccag ccccatcagt gccccggtat taccggcact gacacaggct
    13141 tgcgctcgac cttctttcac cagctccagg gccatacgca ttgaactccc acgactagcg
    13201 cggatagctt gcgaaggccg ggcatcactg gcgataactg actgcgcagg aataatctgc
    13261 agacgcgaac gttgttcaaa gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg
    13321 ccgactaaaa gaagagtgag ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact
    13381 gtcacggaag ggccaaaatc ccctcccatg acatctaacg ccagggttag acgtgtcaag
    13441 gtatcgtcgc cgcccggttt ggtctttccc cagttgcctg gggaaaatcc tcactaagct
    13501 tcatcacgca gacgcgtgat tacttagcga tgaccttgcg gccgcggtag taaccgtcgg
    13561 cagtgatatg gtgacgcagg tgtttttcac cagaagtttt gtctacagac aggctggtga
    13621 ctgcggtcag cgcgtcatgg gaacgacgca tgccacgttt ggaacgggtt ggtttattct
    13681 gttgtacggc catggacctt actcctcaat tacggtttat ccccgatcca acggggagca
    13741 ccaattactt acgctttaag ctggctaata cggcaaatgg gtttggcttt tgcacttctt
    13801 caggcagttc accaaagacc atgtccgctt cggacacttc acagtgttca gaatcatgca
    13861 ccggaactac cggcaaggcg aggatgattt catcttcaac cattgcaagc agatcgattt
    13921 caccgaattc gttaacctca atcggttcat acgcttccgg cagtgcttca gcctgttcgt
    13981 ctgaacgcac aggactaaaa caatacgttg tgtagacctg atgagtaaac ggcttcccgc
    14041 aacgctgaca ctcgagcgtt accgtcacct tcgcatcgcc gtttaacact gcgagacgtt
    14101 ggttatcgat agcgaacgac atggagcatt ccacatcact gtccacactg actacggatt
    14161 cggcgacgcg ctcaacctga tcaggggtat agataccctg gtaatcaagg cgtttttgag
    14221 ccgtacgaac cggatcgaga gtcaggggta attttacctt ttgcataggg cgcgcatatt
    14281 aactttgtaa cgtcatagag tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa
    14341 ttcgcacaca ttgcgggtta ctgctttatt ttcgttcagt gctgataacg aacatcaacc
    14401 tgcaaaacga agcgcatagt ttaaaatggc tatcatcaat acgctatatc tgatggaaaa
    14461 aaatatgcct aaacttattt tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa
    14521 actgcaaatc tcttttgaat gtgcagcacc agaggtcgac gaaaccccac gcagcgacga
    14581 atcaccgcga cagttggtgc ttcgactggc acaagaaaaa gcgcaatctc tggcgtcagg
    14641 ttatccggat catttaatta ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac
    14701 cggtaaaccg ttaacggaag aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat
    14761 cgtcaccttc tatactggac tggcgttgtt taattcggcg aatgggcatc tacaaacaga
    14821 agtggagcct tttgacgtcc atttccgtca cctgtgcgag gcggagattg ataattacgt
    14881 gcgtaaggag catcccctgc actgcgcggg tagctttaag agtgaaggat ttggcattac
    14941 gctgtttgag cgtttagagg gacgtgaccc taacacgctg gttggtttac cgctaatcgc
    15001 gctgtgtcag atgttacgtc gggaagggaa aaacccgctg atgggatagt tgtttgcgcc
    15061 ggatgcggtt acgtcgcatc cggcaatcgg tgcaggcggc ctgatgcggc gtaaacgcct
    15121 tatcaggcca accgcccatt atcaggctta tatgcttgta ttagcgcgcg ttacgcagct
    15181 tttgcaaaca acgcttcaaa ccttcatcca tcggcgcttc gatacgcatc acctcaccgg
    15241 tccccggatg ggtaaacttc aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc
    15301 ctgcttcagt gagctgtctg tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg
    15361 gatgacccgc atactgtgta tgcacacgga tctgatgagt acgtccggtt accgggctac
    15421 aacgcaccag ggtggcaaat gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt
    15481 tgccttcctg actgacacgc acgatacgtt cgccgctttg cagaatattt ttcaataacg
    15541 gcgcttgaac gctcttcaca tgcgactgcc actgactgcg caccagcgcc aggtaatctt
    15601 tttgcattcc tttttcacgt aattgctcat gcagagaacg caacgccgag cgttttttcg
    15661 ctaccagcaa cacacctgaa gtgtcccggt cgagacgatg aaccagttca aggaaccgcg
    15721 cttccgggcg caacgcccgc aaaccttcga taacgccgaa gcttaaacca ctgccgccat
    15781 gtaccgccgt accggaaggt ttattcagca ccaggatatg atcatcttca tataagatga
    15841 cgtccgccag cgccgccacc ttttgcagat gtggcgaaac cgcctcttct tcccgctcag
    15901 caacgcgaac cggtgggata cgcacctcat cacccgcttc gagtttatat tcaggcttaa
    15961 tacgtttttt gttcacccgc acttcgcctt tacgcaaaat acggtaaatc atactttttg
    16021 gtacgccttt caattgggta cgcaaaaagt tgtcgatacg ttgccccgct tcgtcagcgg
    16081 tgatagcaac aatttttacg gatggagtct ctgttttcat ggtgggcgat tctaattagc
    16141 caacaggatt cgcgccactc atttttctat gcttatattt agttttacac cttattactt
    16201 cactacgtga tcactttatt gatggttatt aaaccaatca ccagcaagaa gtgaaaaatg
    16261 taatgaacgc atcccacaca attaaacacg gagcagtatg atggttaaaa gggtgaaact
    16321 ggcaggtgtg gagcctcgca gatttccggc atcatagtgc ccaatacggg atgcataaag
    16381 ctccctcaga ctggagactc cacaaatgaa atatacaccg gttggcgttg gtatcgcaaa
    16441 acatgtcatt cagattcact tcatcaatga gcacacagat gaagtggttg ataaacagtt
    16501 gcgtagacag gattttctga cgttcttcgg caaccgtgag ccatgcctga ttggtatgga
    16561 ggcctgtgga ggttctcagc actgggcacg ggaactgaca aaacttggtc ataaagtccg
    16621 gttgttgcag gcccgcttcg ttaaggcatt cgtcatgggc aataagaatg atgtgatgga
    16681 tgcccgggct atctggatgg cggttcagca gccgggtaaa gaaatcgccg taaaaacaga
    16741 agaacagcag tcggtactgg ttctgcaccg tacccgcatg caactggtga agttccggac
    16801 cgcacaaatt aatgccctgc acgggacgtt actggagttt ggtgaaacca tccacaaagg
    16861 ccgggcagcg atggagcggg agttccccga agcactggaa cggatgaaag agagactgcc
    16921 accgtatctc attatggttc tggaaaacca gtacaaccga ctgaatgagc tggactcact
    16981 gatagaggat attgaaaaac agcttaccag cgtggcgagg cagaatgaaa cctgtaagc
//
LOCUS       DAJTNN010000080        16764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTNN010000080 DAJTNN010000000
VERSION     DAJTNN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.080"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..356
                     /locus_tag="OO150_002321"
     CDS             <1..356
                     /locus_tag="OO150_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5516447.1"
                     /translation="KPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLSEPQKAG
                     IASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVS
                     RRDQESALACWGIDR"
     gene            655..1092
                     /locus_tag="OO150_002322"
     CDS             655..1092
                     /locus_tag="OO150_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5516448.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKEQRDKATSTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGM
                     GNGESPRLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            1509..2129
                     /locus_tag="OO150_002323"
     CDS             1509..2129
                     /locus_tag="OO150_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCR5516449.1"
                     /translation="MKDQDVRFAVHHKLLKESHLDPDCLVVDEFSISLGASRADIAVI
                     NGVIHGYELKSEYDSLERLPLQIKHYSSVMDKVTLVVAEKHLEGALKLIPGWWGVKTV
                     SVGPKGAILIKHMRGEKLNRNHDTLMLAQLLWKDECIDVLERWGYSKGIKSKPRFELW
                     NIIAENIPIANLRLEVRTALKKRVGWKVKAWQAESAPTNKAVSRLT"
     gene            complement(2071..3138)
                     /locus_tag="OO150_002324"
                     /pseudo
     CDS             complement(2071..3138)
                     /locus_tag="OO150_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
     gene            3551..4096
                     /locus_tag="OO150_002325"
     CDS             3551..4096
                     /locus_tag="OO150_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR5516450.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            4071..5996
                     /locus_tag="OO150_002326"
     CDS             4071..5996
                     /locus_tag="OO150_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5516451.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5993..6199
                     /locus_tag="OO150_002327"
     CDS             5993..6199
                     /locus_tag="OO150_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR5516452.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            6196..7797
                     /locus_tag="OO150_002328"
     CDS             6196..7797
                     /locus_tag="OO150_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5516453.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            7778..9097
                     /locus_tag="OO150_002329"
     CDS             7778..9097
                     /locus_tag="OO150_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5516454.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            9107..9439
                     /locus_tag="OO150_002330"
     CDS             9107..9439
                     /locus_tag="OO150_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5516455.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDSAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            9495..10520
                     /locus_tag="OO150_002331"
     CDS             9495..10520
                     /locus_tag="OO150_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5516456.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGSTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            10562..10957
                     /locus_tag="OO150_002332"
     CDS             10562..10957
                     /locus_tag="OO150_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5516457.1"
                     /translation="MTKDELIVRLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTPLRPENALTGHENEVVSAQTDTVTDAAALVTVVALVTLHTDALHATRDEP
                     VAFVLPETAFRVSAGVAAEMTERGLARMQ"
     gene            10969..11322
                     /locus_tag="OO150_002333"
     CDS             10969..11322
                     /locus_tag="OO150_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR5516458.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            11334..11912
                     /locus_tag="OO150_002334"
     CDS             11334..11912
                     /locus_tag="OO150_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5516459.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            11909..12304
                     /locus_tag="OO150_002335"
     CDS             11909..12304
                     /locus_tag="OO150_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5516460.1"
                     /translation="MKHTELRAAVLDALEKHDSGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            12312..13052
                     /locus_tag="OO150_002336"
     CDS             12312..13052
                     /locus_tag="OO150_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5516461.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDKDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAVVAEITVTDS"
     gene            13068..13490
                     /locus_tag="OO150_002337"
     CDS             13068..13490
                     /locus_tag="OO150_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5516462.1"
                     /translation="MFLKTESFEYNGVSVTLSELSALQRIEHLALLKRQAEQAGSSLN
                     RQVSVEDLVRTGAFLVAMSLWHSHPQKTKMPSMNEAVKQIEQEVLTTWPTEAIAQAEN
                     VVMRLSGMSEFVVNDAPEQADDAGPAEPVSAGKCSTVS"
     gene            13472..13906
                     /locus_tag="OO150_002338"
     CDS             13472..13906
                     /locus_tag="OO150_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5516463.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSIHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13899..16460
                     /locus_tag="OO150_002339"
     CDS             13899..16460
                     /locus_tag="OO150_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5516464.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKVGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SQLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQCRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            16457..>16764
                     /locus_tag="OO150_002340"
     CDS             16457..>16764
                     /locus_tag="OO150_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5516465.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     "
BASE COUNT         4042 a         4086 c         5018 g         3618 t
ORIGIN      
        1 gcaaacctgt cgttccgggc atgaagttgt cgaaggaaaa atgcgaccgg gttaacgcca
       61 ttgagcgtga taaggcgctg gcatgggtgg agaaaaacat cagagtgcca ttgagtgaac
      121 cccagaaagc ggggatcgcg tcattctgtc cgtacaacat tggccccggt aagtgttttc
      181 cgtcgacgtt ttataaacga attaatgcag gtgatcgcag gggagcgtgt gaggcgattc
      241 gctggtggat taaggacggt ggcagagact gccgtattcg ctcaaacaac tgttacggtc
      301 aggtatcccg tcgcgaccag gagagtgcgc tggcgtgctg gggaattgac agataagcag
      361 aatattttgc tgaaaaatgc ggtttgctca cacggacgga taacacgaaa tcctgcgaac
      421 tgacaaaaac taagagaata aaagtaaaaa ccccgtttgt tggctgcaag cggggttttg
      481 tgtttcctga ctccggaaaa gtcaaaggag aaagtgtgtt tgattttagc aaactgattc
      541 gggagattcg agtgatggct gaaaaattat ccacctggaa gttcattctt atctggctgg
      601 tgtttgtgat tatggcctcc ggttatttca tcggtcagat acgctggtgg tgaaatgaac
      661 cgcgtactgt gcgtggtcat cattgccctg ctggtggcct gtggtgcgct tagtctgggg
      721 ctgaatcatt accgtgataa cgccataacc tacaaagagc agcgcgataa agccacatcc
      781 acaatcgctg atatgcagaa gcgtcaacgt gacgtagcag aactcgatgc cagatataca
      841 aaggagcttg ctgatgctaa cgcgactatc gaaagcctcc gtgctgatgt ttctgctggg
      901 cgtaagcgcc tgcaagtcgc cgctacctgt gcaaagtcaa cgaccggagc cagcggcatg
      961 ggcaatggag aaagcccaag acttacagca gatgctgaac tcaattatta ccgtctccga
     1021 agtggaatcg acaagataac cgcgcaggtc aactacctgc aggagtacat caggacgcag
     1081 tgcttaaaat aattttaatt tcactgaaat ttaatacgtg actttcagga aaatgcctcg
     1141 cagatgcggg gcatttttgt acaggtattt caccgcgcac cgcagtgcac tcaaccacgt
     1201 cgaaccaaac cctttggaat gagcctttga gtagtcagtt agtgctggtg agccttgacg
     1261 ggctgatctc ctatgcagca aaggttcatc tcaaagtaag gcgaacgcta tgacaaacca
     1321 agattctata gacctatctg atcttcgtgg aatggtcagt tttccagaca aaaggtaatc
     1381 accatagtcg tatggctatg aatcttgttg ctgcagataa gcattttgtg attgaagtgg
     1441 tcaaatctct caaatgcagt aaaatgcagt gcgctataat tcaataacgg agggagtaag
     1501 gagaagtcat gaaagatcaa gatgttaggt tcgcggtgca tcataagctt ttgaaagaat
     1561 cgcatttaga tccagactgc cttgtggtcg atgaattttc catatccctt ggcgccagta
     1621 gagcagacat tgctgtaata aatggtgtta tacacgggta cgagctcaaa agtgaatatg
     1681 actctttgga gcgtttgcct cttcaaatca agcattattc ttctgtaatg gacaaggtta
     1741 ctcttgtcgt agctgagaaa catcttgagg gagcattaaa gttaatccca ggttggtggg
     1801 gcgttaaaac ggtttctgtt gggccaaaag gcgccattct tataaagcac atgcgtggag
     1861 aaaagcttaa tcgaaaccat gacacattga tgctcgctca attgctttgg aaagatgaat
     1921 gtatcgacgt acttgaacga tggggctatt ccaaaggaat caaaagcaag ccccgatttg
     1981 agttatggaa tattattgcg gaaaatattc caatagcgaa tctcaggctt gaagtcagaa
     2041 cagccttaaa gaaacgcgta ggctggaaag ttaaggcttg gcaggctgag tctgcgccaa
     2101 ccaataaagc tgtctcacga ctaacgtaat atggtgcgta tgtgcaactt tacgccattc
     2161 tttagagccg ccagatttgt tagcgcctaa tgatctttgg taaatgtaat catcccccca
     2221 actaaatttg gaaccaaaga cttgatactc tggcgaacta acaagggtag tacataagtt
     2281 tttagtttgg ccccatccat ttcctttaac tgcggtacct tttacaaaga tccatgaggt
     2341 atcgttcgaa tatctcactg agacatactg agacatgaag cgtgggtcta cgctcgtaat
     2401 ggtagagcta gcggtgggat aatcactaaa acttggcgtt cttccattgc taaaattctg
     2461 tactacgtac atccaaagat cgtattcatg gcgcggaata tgatgaactt gatgttgcgg
     2521 tatccctgcc tgtgatgctg ggtatgcggt tgaagataaa atcaagtttc tccacggagc
     2581 ttggcctgat aatgtgttga ccatgcttaa tgcttgttat tttaaactat cagttgcgtt
     2641 ttgaatatct ccaaaatcaa tgattacatc aattaaacta ggatgaatat ttaagtggtt
     2701 aaccagactt gtgaattggt gccatgtctg gggggtgata gatatagcga gcccattgat
     2761 taagttgcgt tgaacagcat gtatatagtt tgttgaatat gcgggagaaa caacagggat
     2821 aatttcctta ccatttactc tagcatcttg gatacacata tctagaggat gatggcggct
     2881 cgaaccatgt ttatctaaat atttaacatc cagcaaaaca ggacgattgg ctttccagga
     2941 tgccgcaagg ttaataccga agtcagataa gtaagaactc aaactcttct tgtagcattc
     3001 gttttcataa tcccagtcta tgtctggaat ggtaatgatc ggagtaaaac cagagagcgt
     3061 agtttgatct aaaagcatca gagattcata ttcagcaggc ttccatttca gctgtggata
     3121 atattgatgt tgactcatta aaactcctta aaatctttac ttatcctaag tatagtgtta
     3181 tggcgtaacg ccaattgttt ttatacactt gaaaacattg ggaaaagttg agctatatct
     3241 aaatttacaa aattggatgt tatagaaatc gaggtttcaa ctaggcatgg aactgcaaaa
     3301 tttttaatgc ctgcacgcga aagtcgttgg cgggtccttt ccggtgatcc agaccgttac
     3361 ggggcggcga cctcgcgggt tttcgctatt tatgaaaatt ttccggttta aggcgtttcc
     3421 gttcttcttc gtcgtaactt aatgttttta tttaaaatac cccctgaaaa gaaaggaaac
     3481 gacaggtgct gaaaacgaac ttttgggcct ttgtcgtttc ctttctctgt ttttggccgt
     3541 ggaatgaaca atggaagtca acaaaaagca gctggctgac attttcggtg cgagtatccg
     3601 taccattcag aactggcagg aacagggaat gcccgttctg cgaggcggtg gcaagggtaa
     3661 tgaggtgctt tatgactctg ccgccgttat aaaatggtat gccgaaaggg atgctgaaat
     3721 tgagaacgaa aagctgcgcc gggaggttga agaactgcgc caggccagcg aggcagatct
     3781 ccagccaggg actattgagt acgaacgcca tcgacttacg cgtgcgcagg ccgacgcaca
     3841 ggaactgaag aatgccagag actcagctga agtggtggaa accgcattct gtactttcgt
     3901 gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggatccccc tgtcggtgca
     3961 gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg atatcatcaa
     4021 agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga gtgaatatat
     4081 cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc tcactgttca
     4141 ggccggagcc acagaccgcc gttgaatggg cggatgctaa ttactatctc ccgaaagaat
     4201 ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc atgaatgcga
     4261 tgggcagcga ctacatccgt gaggtgaatg tggtgaagtc tgcccgtgtc ggttattcca
     4321 aaatgctgct gggtgtttat gcctacttta tagagcataa gcagcgcaac acccttatct
     4381 ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag ccgactattc
     4441 gtgatattcc gtcgctgctg gcgctggccc cgtggtatgg caaaaagcac cgggataaca
     4501 cgctcaccat gaagcgtttc actaatgggc gtggcttctg gtgcctgggc ggtaaagcgg
     4561 caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta tgatgaactt gctgcctttg
     4621 atgaggatat tgaacaggaa ggctctccga cgttccttgg cgacaaacgt attgaaggct
     4681 cggtctggcc aaagtccatc cgtggctcca cgcccaaagt gagaggcacc tgccagattg
     4741 agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc ccgcactgcg
     4801 gggaggagca gtatcttaaa tttggcgaca aagagacgcc gtttggcctc aaatggacgc
     4861 cggatgaccc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc atccgccagc
     4921 aggagctgga ctttactgat gcccgttata tctgcgaaaa gaccgggatc tggacccgtg
     4981 atggcattct ctggttttcg tcatccggtg aagagattga gccacctgac agtgtgacct
     5041 ttcacatctg gacagcgtac agcccgttca ccacctgggt gcagattgtc aaagactgga
     5101 tgaaaacgaa aggggatacg ggaaaacgta aaaccttcgt aaacaccacg ctcggtgaga
     5161 cgtgggaggc gaaaattggc gaacgtccgg atgctgaagt gatggcagag cggaaagagc
     5221 attattcagc gcccgttcct gaccgtgtgg cttacctgac cgccggtatc gactcccagc
     5281 tggaccgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc tggctgattg
     5341 accggcagat tattatgggc cgccacgacg atgaacagac gctgctgcgt gtggatgagg
     5401 ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcgatatcc cgtatctgct
     5461 gggatactgg cgggattgac ccgaccattg tgtatgaacg ctcgaaaaaa catgggctgt
     5521 tccgggtgat ccccattaaa ggggcatccg tctacggaaa gccggtggcc agcatgccac
     5581 gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg tacggatacc gcgaaagagc
     5641 agatttataa ccgcttcaca ctgacgccgg aaggggatga accgcttccc ggtgccgttc
     5701 acttcccgaa taacccggat atttttgatc tgaccgaagc gcagcagctg actgctgaag
     5761 agcaggtcga aaaatgggtg gatggcagga aaaaaatact gtgggacagc aaaaagcgac
     5821 gcaatgaggc gctcgactgc ttcgtttatg cgctggcggc gctgcgcatc agtatttccc
     5881 gctggcagct ggatctcagt gcactgctgg cgagcctgca ggaagaggat ggtgcagcaa
     5941 ccaacaagaa aacactggca gattacgccc gtgccttatc cggagaggat gaatgacgcg
     6001 acaggaagaa cttgccgctg cccgtgcggc actgcatgac ctgatgacag gaaaacgggt
     6061 ggcaacggta cagaaagacg gacggagagt ggagtttacg gccacttccg tgtctgacct
     6121 gaaaaaatac attgcggagc tggaagtgca gaccggcatg acacagcgac gcaggggacc
     6181 tgcaggattt tatgtatgaa aacgtccacc attcccaccc ttctggggcc ggacggcatg
     6241 acatcgctgc gtgaatatgc cggttatcac ggcggtggca gcggatttgg agggcagttg
     6301 cgggcgtgga acccaccgag tgaaagtgtg gatgcagccc tgctgcccaa ctttacccgt
     6361 ggcaatgccc gcgcagacga tctggtacgc aataacggct atgccgccaa cgccatccag
     6421 ttgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc
     6481 tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg
     6541 aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc
     6601 atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc
     6661 acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag
     6721 cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt
     6781 aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg
     6841 ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcctc gttcattcac
     6901 gtttttgaac ccgtggagga cgggcagact cgcggtgcaa atgtgtttta cagcgtgatg
     6961 gagcagatga agatgctcga cacgctgcag aacacgcagc tgcagagcgc cattgtgaag
     7021 gcgatgtatg ccgccaccat tgagagtgag ctggatacgc agtcagcgat ggattttatt
     7081 ctgggcgcga acagtcagga gcagcgggaa aggctgaccg gctggattgg tgaaattgcc
     7141 gcgtattacg ccgcagcgcc ggtccggctg ggaggcgcaa aagtaccgca cctgatgccg
     7201 ggtgactcac tgaacctgca gacggctcag gatacggata acggctactc cgtgtttgag
     7261 cagtcactgc tgcggtatat cgctgccggg ctgggtgtct cgtatgagca gctttcccgg
     7321 aattacgccc agatgagtta ctccacggca cgggccagtg cgaacgagtc gtgggcgtac
     7381 tttatggggc ggcgaaaatt cgtcgcatcc cgtcaggcga gccagatgtt tctgtgctgg
     7441 ctggaagagg ccatcgttcg ccgcgtggtg acgttacctt caaaagcgcg cttcagtttt
     7501 caggaagccc gcagtgcctg ggggaactgc gactggatag gctccggtcg tatggccatc
     7561 gatggtctga aagaagttca ggaagcggtg atgctgatag aagccggact gagcacctac
     7621 gagaaagagt gcgcgaaacg cggtgacgac tatcaggaaa tttttgccca gcaggtccgt
     7681 gaaacgatgg agcgccgtgc agccggtctt aaaccgcccg cctgggcggc tgcagcattt
     7741 gaatccgggc tgcgacaatc aacagaggag gagaagagtg acagcagagc tgcgtaatct
     7801 cccgcatatt gccagcatgg cctttaatga gccgctgatg cttgaacccg cctatgcgcg
     7861 ggttttcttt tgtgcgcttg caggccagct tgggatcagc cgcctgacgg atgcggtgtc
     7921 cggcgacagc ctgactgccc aggaggcact cgcgacgctg gcattatccg gtgatgatga
     7981 cggaccacga caggcccgca gttatcaggt catgaacggc atcgccgtgc tgccggtgtc
     8041 cggcacgctg gtcagccgga cgcgggcgct gcagccgtac tcggggatga ccggttacaa
     8101 cggcattatc gcccgtctgc aacaggctgc cagcgatccg atggtggacg gcattctgct
     8161 cgatatggac acgcccggcg ggatggtggc gggggcattt gactgcgctg acatcatcgc
     8221 ccgtgtgcgt gacataaaac cggtatgggc gcttgccaac gacatgaact gcagtgcagg
     8281 tcagttgctt gccagtgccg cctcccggcg tctggtcacg cagaccgccc ggacaggctc
     8341 catcggcgtc atgatggctc acagtaatta cggtgctgcg ctggagaaac agggtgtgga
     8401 aatcacgctg atttacagcg gcagccataa ggtggatggc aacccctaca gccatcttcc
     8461 ggatgacgtc cgggagacac tgcagtcccg gatggacgca acccgccaga tgtttgcgca
     8521 gaaggtgtcg gcatataccg gcctgtccgt gcaggctgtg ctggataccg aggctgcagt
     8581 gtacagcggt caggaggcca ttgatgccgg actggctgat gaacttgtta acagcaccga
     8641 tgcgatcacc gtcatgcgtg atgcactgga tgcacgtaaa tcccgtctct caggagggcg
     8701 aatgaccaaa gagactcaat caacaactgt ttcagccact gcttcgcagg ctgacgttac
     8761 tgacgtggtg ccagcgacgg agggcgagaa cgccagcgcg gcgcagccgg acgtgaacgc
     8821 gcagatcacc gcagcggttg cggcagaaaa cagccgcatt atggggatcc tcaactgtga
     8881 ggaggctcac ggacgcgaag aacaggcacg cgtgctggca gaaacccccg gtatgaccgt
     8941 gaaaacggcc cgccgcattc tggccgcagc accacagagt gcacaggcgc gcagtgacac
     9001 tgcgctggat cgtctgatgc agggggcacc ggcaccgctg gctgcaggta acccggcatc
     9061 tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg atgtttatga cgagcaaaga
     9121 aacctttacc cattaccagc cgcagggcaa cagtgacccg gctcataccg caaccgcgcc
     9181 cggcggattg agtgcgaaag cgcctgcaat gaccccgctg atgctggaca cctccagccg
     9241 taagctggtt gcgtgggatg gcaccaccga cagtgctgcc gttggcattc ttgcggttgc
     9301 tgctgaccag accagcacca cgctgacgtt ctacaagtcc ggcacgttcc gttatgagga
     9361 tgtgctctgg ccggaggctg ccagcgacga gacgaaaaaa cggaccgcgt ttgccggaac
     9421 ggcaatcagc atcgtttaac tttacccttc atcactaaag gccgcctgtg cggctttttt
     9481 tacgggattt ttttatgtcg atgtacacaa ccgcccaact gctggcggca aatgagcaga
     9541 aatttaagtt tgatccgctg tttctgcgtc tctttttccg tgagagctat cccttcacca
     9601 cggagaaagt ctatctctca caaattccgg gactggtaaa catggcgctg tacgtttcgc
     9661 cgattgtttc cggtgaggtt atccgttccc gtggcggctc cacctctgaa tttacgccgg
     9721 gatatgtcaa gccgaagcat gaagtgaatc cgcagatgac cctgcgtcgc ctgccggatg
     9781 aagatccgca gaatctggcg gacccggctt accgccgccg tcgcatcatc atgcagaaca
     9841 tgcgtgacga agagctggcc attgctcagg tcgaagagat gcaggccgtt tctgccgtgc
     9901 tcaagggcaa atacaccatg accggtgaag ccttcgatcc ggttgaggtg gatatgggcc
     9961 gcagtgcggc gaacaacatc acgcagtccg gcagcacgga gtggagcaag cgtgacaagt
    10021 ccacgtatga cccgaccgac gatatcgaag cctacgcgct gaacgccagc ggcgtggtga
    10081 atatcatcgt gtttgatccg aaaggctggg cgctgttccg ttccttcaaa gccgtcaggg
    10141 agaagctgga tacccgtcgc ggctctcatt ccgagctgga gacagcggtg aaagacctgg
    10201 gtaaagcggt gtcctataag gggatgtatg gcgatgtggc catcgtcgtg tattccggac
    10261 agtacgtgga aaacggcgtc aaaaagaact tcctgccgga caacacgatg gtactgggga
    10321 acactcaggc acgcggtctg cgcacctatg gctgcattca ggatgcggac gcacagcgcg
    10381 aaggcattaa cgcctctgcc cgttacccga aaaactgggt gaccaccggc gatccggcgc
    10441 gtgagttcac catgattcag tcagcaccgc tgatgctgct ggctgaccct gatgagttcg
    10501 tgtccgtaca actggcgtaa tcgtggccct tcggggccat tttctctctg tggaggagtc
    10561 catgacgaaa gatgaactga ttgtccgtct ccggtcgctg ggtgagcaac tgaaccgtga
    10621 tgtcagcctg acggggacga aagaagaact ggcgctccgt gtggcagagc tggaagagga
    10681 gcttgatgac acggatgacg ctgccggtca ggatacccct ctcagaccgg aaaatgcgct
    10741 gaccggacat gaaaatgagg tggtatcagc acagacggat accgtgactg atgcggctgc
    10801 tctggtcacg gttgtggcac tggtgacgct gcataccgat gcacttcacg ccacgcggga
    10861 tgaacctgtg gcatttgtgc tgccggaaac ggcgttccgt gtctctgccg gtgtggcagc
    10921 tgaaatgaca gagcgcggcc tggccagaat gcaataacgg gaggcgctgt ggctgatttc
    10981 gataacctgt tcgatgctgc cattgcccgc gccgatgaaa cgatacgcgg gtacatggga
    11041 acgtcagcca ccatgacatc cggtgagcag tccggcgcag taatacgtgg tgtttttgat
    11101 gaccctgaaa atatcagcta tgccggacag ggcgtacgcg ttgaaggctc cagcccgtcc
    11161 ctgtttgtcc ggactgatga ggtgcggcag ctgcggcgcg gcgacacgct gaccatcggt
    11221 gaggaaaact tctggataga ccggatttcg ccggatgatg gcggaagctg tcatctctgg
    11281 cttgggcggg gcgtaccgcc tgccgttaac cgtcgccgct gaaaggggga tgtatggcca
    11341 taaaaggtct tgagcaggcc gttgaaaacc tcagccgtat cagtaaaacg gcggtgcccg
    11401 gtgccgccgc aatggccatt aaccgcgttg cttcatccgc gatatcgcag tcggcgtcac
    11461 aggttgctcg tgagacaaag gtacgccgga aactggtaaa ggaaagggcc aggctgaaaa
    11521 gggccacggt caaaaatccg caggccagaa tcaaagttaa ccggggggat ttgcccgtaa
    11581 tcaagctggg taatgcgcgg gttgtccttt cgcgccgcag gcgtcgtaaa aaggggcagc
    11641 gttcatccct gaaaggtggc ggcagcgtgc ttgtggtggg aaaccgtcgt attcccggcg
    11701 cgtttattca gcaactgaaa aatggccgct ggcatgtcat gcagcgtgtg gccgggaaaa
    11761 accgttaccc cattgatgtg gtgaaaatcc cgatggcggt gccgctgacc acggcgttta
    11821 aacagaatat tgaacggata cggcgtgaac gtcttccgaa agagctgggc tatgcgctgc
    11881 agcatcaact gagaatggta ataaagcgat gaaacatact gaactccgtg cagccgtact
    11941 ggatgcactg gagaagcatg acagcggggc gacgcttttt gatggtcgcc ccgctgtttt
    12001 tgatgaggaa gattttccgg caattgccgt ttatctcacc ggcgctgaat acacgggcga
    12061 agagctggac agcgatacct ggcaggcgga gctgcatatt gaagttttcc tgcctgctca
    12121 ggtgccggat tcagagctgg atgcgtggat ggagtcccgg atttatccgg tgatgagcga
    12181 tatcccggca ctgtcagatt tgatcaccag tatggtggcc agtggctatg actaccggcg
    12241 cgacgatgat gcgggcctgt ggagttcagc cgatctgact tatgtcatta cctatgaaat
    12301 gtgaggacgc tatgcctgta ccaaatccta caatgccggt gaaaggtgcc gggaccaccc
    12361 tgtgggttta taaggggagc ggtgaccctt atgcgaaccc gctttcagac gttgactggt
    12421 cgcgtctggc aaaagttaaa gacctgacgc ccggcgaact gaccgctgag tcctatgacg
    12481 acagctatct cgatgataaa gatgcagact ggactgcgac cgggcagggg cagaaatctg
    12541 ccggagatac cagcttcacg ctggcgtgga tgcccggaga gcaggggcag caggcgctgc
    12601 tggcgtggtt taatgaaggt gatacccgtg cctataaaat ccgcttcccg aacggcacgg
    12661 tcgatgtgtt ccgtggctgg gtcagcagta tcggtaaggc ggtgacggcg aaggaagtga
    12721 ttacccgcac ggtgaaggtc accaatgtgg gacgtccgtc gatggcagaa gatcgcagca
    12781 cggtaacagc ggcaaccggc atgaccgtga cgcctgccag cacttcggtg gtgaaagggc
    12841 agagcaccac gctgaccgtg gcattccagc cggaaggcgc aaccgacaag agcttccgtg
    12901 cggtgtctgc ggataaaaca aaagccaccg tgtcggtcag tggtatgacc atcaccgtga
    12961 aaggtgttgc tgcaggcaag gtcaacattc cggttgtatc cggtaatggt gaacttgctg
    13021 tggttgcaga aatcaccgtc accgacagtt aatccggaga gtcagcgatg ttcctgaaaa
    13081 ccgaatcatt tgaatataac ggtgtgagcg tcacgctttc tgaactgtca gccctgcagc
    13141 gaattgagca tctcgccctg ctgaaacgac aggcagaaca ggcgggatcc agtctcaatc
    13201 gacaggtgag cgtggaagat ctcgtcagaa ccggtgcttt tctggtggcg atgtccctgt
    13261 ggcatagcca tccgcagaag acaaagatgc cgtccatgaa tgaagccgtt aaacaaattg
    13321 agcaggaagt gcttaccacc tggcccacag aggcaattgc tcaggctgaa aatgtggtaa
    13381 tgcgtctgtc cggtatgtct gagtttgttg tgaatgatgc acctgaacag gcagatgacg
    13441 ccgggccagc agagcctgtt tctgcgggaa agtgttcgac ggtgagctga gttttgccct
    13501 gaaactggcg cgtgagatgg ggcgacccga ctggcgcgcc atgcttgccg ggatgtcatc
    13561 cacggagtat gccgactggc accgctttta cagtatccat tattttcatg atgttctgct
    13621 ggatatgcac ttttccgggc tgacgtacac cgtactcagc ctgtttttca gcgatccgga
    13681 tatgcatccg ctggatttca gtctgctgaa ccggcgcgag gctgacgaag agcctgaaga
    13741 tgatgtgctg atgcagaaag cggcagggct tgccggaggc gtccgctttg gcccggacgg
    13801 gaatgaagtt atccccgctt ccccggatgt ggcggacatg acggaggatg acgtaatgct
    13861 gatgacagta tcagaaggga tcgcaggagg agtccggtat ggctgaaccg gtaggcgatc
    13921 tggtcgttga tttgagtctg gatgcggcca gatttgacga gcagatggcc agagtcaggc
    13981 gtcatttttc cggtacggaa agtgatgcga aaaaaacagc ggcagtcgtt gaacagtcga
    14041 tgaaccggca ggcgctggct gcacagaaag tgggaatttc cgtcgggcag tataaagccg
    14101 ccatgcgtat gctgcctgcg cagttcactg acgtggccac gcagcttgca ggcgggcaaa
    14161 gtccgtggct gatcctgctg caacaggggg gtcaggtgaa ggactccttc ggcgggatga
    14221 tccccatgtt ccgggggctt gccggtgcga tcaccctgcc gatggtgggg gccacctcgc
    14281 tggcggtggc gaccggagcg ctggcgtatg cctggtatca gggcaactca accctgtccg
    14341 atttcaacaa aacgctggtc ctttccggca atcaggcggg actgacggca gatcgtatgc
    14401 tggtcctgtc cagagccggg caggcggcag ggctgacgtt taaccagacc agcgagtcac
    14461 tcagcgcact ggttaaggcg ggggtaagcg gtgaggctca gattgcgtcc atcagccaga
    14521 gtgtggcgcg tttctcctct gcatccggcg tggaggtgga caaggtcgct gaagccttcg
    14581 ggaagctgac cacagacccg acgtcggggc tgacggcgat ggcacgccag ttccataacg
    14641 tgacggcgga gcagattgcg tatgttgctc agttgcagcg ttccggcgat gaagccgggg
    14701 cattgcaggc ggcgaacgag gccgcaacga aagggtttga tgaccagacc cgacgcctga
    14761 aagagaacat gggcacgctg gaaacctggg cagacaggac agcacgggca ttcaaatcca
    14821 tgtgggatgc ggtgctggat attggtcgtc ctgataccgc tcaggagatg ctgattaagg
    14881 cagaggctgc gtttaagaaa gcagacgaca tctggaatct gcgcaaggat gattattttg
    14941 ttaacgatga agcgcgggcg cgttactggg atgatcgtga aaaggcccgt cttgcgcttg
    15001 aagccgcccg aaagaaggct gagcagcaga ctcaacagga caaaaatgcg cagcagcaga
    15061 gcgatactga agcgtcacag ctgaaatata ccgaagaggc gcagaaggct tacgaacggc
    15121 tgcagacgcc gctggagaaa tataccgccc gtcaggaaga actgaacaag gcactgaaag
    15181 acgggaaaat cctgcaggcg gattacaaca cgctgatggc ggcggcgaaa aaggattatg
    15241 aagcgacgct gaaaaagccg aaacagtccg gcgtgaaggt gtctgcgggc gatcgtcagg
    15301 aagacagtgc tcatgctgcc ctgctgacgc ttcaggcaga actccggacg ctggagaagc
    15361 atgccggagc aaatgagaaa atcagccagc agcgccggga tttgtggaag gcggagagtc
    15421 agttcgcggt actggaggag gcggcgcaat gtcgccagct gtctgcacag gagaaatccc
    15481 tgctggcgca taaagatgag acgctggagt acaaacgcca gctggctgca cttggcgaca
    15541 aggttacgta tcaggagcgc ctgaacgcgc tggcgcagca ggcggataaa ttcgcacagc
    15601 agcaacgggc aaaacgggcc gccattgatg ctaaaagccg ggggctgact gaccggcagg
    15661 cagaacggga agccacggaa cagcgcctga aggaacagta tggcgataat ccgctggcgc
    15721 tgaataacgt catgtcagag cagaaaaaga cctgggcggc tgaagaccag cttcgcggga
    15781 actggatggc aggcctgaag tccggctgga gtgagtggga agagagcgcc acggacagta
    15841 tgtcgcaggt aaaaagtgca gccacgcaga cctttgatgg tattgcacag aatatggcgg
    15901 cgatgctgac cggcagtgag cagaactggc gcagcttcac ccgttccgtg ctgtccatga
    15961 tgacagaaat tctgcttaag caggcaatgg tggggattgt cgggagtatc ggcagcgcca
    16021 ttggcggggc tgttggtggc ggcgcatccg cgtcaggcgg tacagccatt caggccgctg
    16081 cggcgaaatt ccattttgca accggaggat ttacgggaac cggcggcaaa tatgagccag
    16141 cggggattgt tcaccgtggt gagtttgtct tcacgaagga ggcaaccagc cggattggcg
    16201 tggggaatct ttaccggctg atgcgcggct atgccaccgg cggttatgtc ggtacaccgg
    16261 gcagcatggc agacagccgg tcgcaggcgt ccgggacgtt tgagcagaat aaccatgtgg
    16321 tgattaacaa cgacggcacg aacgggcaga taggtccggc tgctctgaag gcggtgtatg
    16381 acatggcccg caagggtgcc cgtgatgaaa ttcagacaca gatgcgtgat ggtggcctgt
    16441 tctccggagg tggacgatga agaccttccg ctggaaagtg aaacccggta tggatgtggc
    16501 ttcggtccct tctgtaagaa aggtgcgctt tggtgatggc tattctcagc gagcgcctgc
    16561 cgggctgaat gccaacctga aaacgtacag cgtgacgctt tctgtccccc gtgaggaggc
    16621 cacggtactg gagtcgtttc tggaagagca cgggggctgg aaatcctttc tgtggacgcc
    16681 gccttatgag tggcggcaga taaaggtgac ctgcgcaaaa tggtcgtcgc gggtcagtat
    16741 gttgcgtgtt gagttcagcg caga
//
LOCUS       DAJTNN010000081        16763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTNN010000081 DAJTNN010000000
VERSION     DAJTNN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.081"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            189..2036
                     /gene="ldtD"
                     /locus_tag="OO150_002341"
     CDS             189..2036
                     /gene="ldtD"
                     /locus_tag="OO150_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5516466.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2217..2765
                     /locus_tag="OO150_002342"
     CDS             2217..2765
                     /locus_tag="OO150_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5516467.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPPPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2792..3439
                     /gene="gloC"
                     /locus_tag="OO150_002343"
     CDS             2792..3439
                     /gene="gloC"
                     /locus_tag="OO150_002343"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5516468.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3661..4851)
                     /gene="aspC"
                     /locus_tag="OO150_002344"
     CDS             complement(3661..4851)
                     /gene="aspC"
                     /locus_tag="OO150_002344"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5516469.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARMAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5036..6124)
                     /gene="ompF"
                     /locus_tag="OO150_002345"
     CDS             complement(5036..6124)
                     /gene="ompF"
                     /locus_tag="OO150_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5516470.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="OO150_002346"
     CDS             complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="OO150_002346"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5516471.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8295..9497)
                     /gene="pncB"
                     /locus_tag="OO150_002347"
     CDS             complement(8295..9497)
                     /gene="pncB"
                     /locus_tag="OO150_002347"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5516472.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9763..12375
                     /gene="pepN"
                     /locus_tag="OO150_002348"
     CDS             9763..12375
                     /gene="pepN"
                     /locus_tag="OO150_002348"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5516473.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12418..13169)
                     /gene="ssuB"
                     /locus_tag="OO150_002349"
                     /pseudo
     CDS             complement(12418..13169)
                     /gene="ssuB"
                     /locus_tag="OO150_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13166..13903)
                     /gene="ssuC"
                     /locus_tag="OO150_002350"
                     /pseudo
     CDS             complement(13166..13903)
                     /gene="ssuC"
                     /locus_tag="OO150_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(13968..15113)
                     /gene="ssuD"
                     /locus_tag="OO150_002351"
                     /pseudo
     CDS             complement(13968..15113)
                     /gene="ssuD"
                     /locus_tag="OO150_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15110..16069)
                     /gene="ssuA"
                     /locus_tag="OO150_002352"
     CDS             complement(15110..16069)
                     /gene="ssuA"
                     /locus_tag="OO150_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5516474.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALCIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16062..16637)
                     /gene="ssuE"
                     /locus_tag="OO150_002353"
     CDS             complement(16062..16637)
                     /gene="ssuE"
                     /locus_tag="OO150_002353"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5516475.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4192 a         4341 c         4276 g         3954 t
ORIGIN      
        1 acggcgcgct tttctttaaa cttctttaca ttaggttatg taaaaacgta tcggcgttta
       61 tatactgaag ataagcctga tgagtaacag gcttgctcgt catactttcg tgagtattgg
      121 cgttgtacag gcaagtcgta aaataacagc ctggctattc agagtatgat aaaaacaggg
      181 ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc agtttgtgcc
      241 tggccgtaac attcgctcca ctgttcaatg cgcaggccga tgagcctgaa gtaatccctg
      301 gcgacagccc ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg caagccacgg
      361 caataatggc ggggatccag ccattgcctg aaggtgcggc agaaaaagcc cgcacgcaaa
      421 tcgaatctca attacccgca ggttacaagc cggtttatct taaccagctt caactgttgt
      481 atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa gccttccagc
      541 aacagctggc agaggtggcg attgccggtt tccagccgca gtttaataaa tgggtagagt
      601 tactgaccga tcctggtgtt aacgggatgg cacgcgacgt agtgctctct gatgcgatga
      661 tgggctatct ccatttcatt gcaaatattc cggtcaaagg cactcgctgg ctatatagca
      721 gtaaacctta tgcacttgca acgccgccgc tctcggtgat taaccaatgg cagctggcgc
      781 tggataaagg tcaattgcct acgtttgttg caggactggc accgcagcat ccgcaatatg
      841 cggcgatgca tgaatcgtta ctggccttac tcagtgacac caaaccgtgg ccccaactga
      901 ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg ttgcgcgaaa
      961 tattgcaacg cacaggcatg ttggacgggg ggccgaaaat tactctacct ggcgatgaca
     1021 cgccaactga cgcggtagtc agcccatccg ctgttactgt tgaaacagca gaaactaagc
     1081 cgatggacaa gcaaacgacg tctcgtagta aacctgcgcc tgccgttcgc gccgcctacg
     1141 ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg ggggcagatg
     1201 gtgctattgg cccggcaaca cgtgactggt taaacgtaac gcccgcccag cgtgctggcg
     1261 tgttggctct caacatccag cgattgcgct tgctgccaac agagctttct accgggatca
     1321 tggttaacat tccggcctat tcgctggtct actatcagaa cggcaatcag gtgctggatt
     1381 cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt gcccttaaca
     1441 acgtagtggt aaacccgccg tggaacgtac ctccaactct ggcacgcaaa gatattctgc
     1501 caaaagtgcg caacgatccg ggatatctcg aaagccatgg ctatacggtg atgcgtggct
     1561 ggaacagcag agaagcgatt gacccatggc aggttgactg gtctacaatc acggcctcga
     1621 atttaccgtt ccgcttccag caggctccag gcccacggaa ctcgctgggg cgctataaat
     1681 tcaatatgcc gagttcagag gccatttatt tgcatgacac gccgaaccac aatctgttca
     1741 agcgtgatac acgcgcattg agctcaggct gtgtacgagt gaataaagct tccgatctgg
     1801 cgaatatgct gttgcaggat gcaggctgga atgacaaacg tatttctgat gcgctgaagc
     1861 agggtgatac acgttacgtc aatattcggc agtcgattcc ggtgaatctc tactacctga
     1921 cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac agatatttac aattatgatc
     1981 tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga acaattaatc aggtaaatga
     2041 agtagttcgg ggaaatcagt tgtcgtaata attagcatga ttgggggcga ttctctgcag
     2101 cccccgtcac tgctggggtt gagtcatctt gacgtctgct ttacgggcgg ttaaggtgcc
     2161 tcttgtgcgc cagaagtgca tataaacgat aacattgacc tgtagacttg attatcatgg
     2221 acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca ctcggtgccg
     2281 ccatcctgcc gccccctgcg tttgcaacac tctctacccc acgcccgcgc attttgacac
     2341 tcaataatct tcataccgga gagtcaatca aagcggagtt tttcgatggc agaggctata
     2401 ttcaggaaga attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg aacaaaataa
     2461 agtccatcga cccaggatta ttcgaccagt tgtatcgcct gcaagggttg ttaggcacgc
     2521 gcaaaccggt gcaactcatt tccggttatc gttccattga taccaacaat gaactacgcg
     2581 cccgcagccg tggagtagcg aagaaaagct atcacactaa aggccaggcg atggatttcc
     2641 atattgaagg tatcgcgtta agcaatattc gcaaagccgc gttatctatg cgcgcaggtg
     2701 gtgtaggata ttacccacgt agtaactttg tgcatattga taccgggcca gcacggcact
     2761 ggtagcaatc gcttaacgaa acaggggcag tatgaactat cgtattattc cggtcaccgc
     2821 attctcccag aactgttcat taatctggtg tgaacaaacc cgtctggccg cactggtcga
     2881 tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat gacagcggcc tgacactgat
     2941 gcagatcctg ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg aactggcgca
     3001 acattacggc gtgccggttt ttggcccgga aaaagaagat gagttctggc tgcaaggctt
     3061 gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag ccgctgacgc cagatcgttg
     3121 gctgaacgaa ggcgatacca tcagcatagg aaatgtgact ttacaggtgt tacattgccc
     3181 tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg gcaaagctgc tgatttctgg
     3241 cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg atcataatca
     3301 actgatttct tcaatcaaag ataaattgct gccactgggg gatgacgtga catttattcc
     3361 gggtcacgga ccattatcca cacttggtta tgaacgcctg cataatccct tcctgcaaga
     3421 cgaaatgccc gtctggtaag gcacataaaa aagcccgctt ttaatgcggg cctggatttc
     3481 tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg gcctacaaaa
     3541 tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag cgcatcaggc
     3601 aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg tttttaatgc
     3661 ttacagcact gccacaatcg cttcgcacag cggagccatg ttatctggtg tcatcccggc
     3721 cacatttacg cgaccagaag caaccgcata tacgccaaac tcttcgcgca gacgcagcac
     3781 ttgttctttt gtcaggccac tgaaggagaa catgccgttc tgtttgatga taaagctgaa
     3841 gtcgcggttt gcgccttttt cctgcagcgt attgacgaac aactgacgca tacgctgaat
     3901 acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaacgcat cgttgctcag
     3961 gatggtggca acaacagaag cgccgtgtgc tggtgggtta gagtagttag cgcgaatcgc
     4021 cgctttcatt tggctgaatg cgcgatcaac ggtttcactg tcggcagcaa ccagagtaca
     4081 agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg caacaatcag
     4141 ctctttatgc atagccgcga aagcgcgcag tccttcagca tcttcttcca gaccacgggc
     4201 aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg agagttgtgc
     4261 cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc agcagccatg
     4321 gaacagcact acgtcgccag cctgagcttc attcaggctg ttaatcagtg catcgaagtc
     4381 aagagtgtga ttttccgcat cataataagc gtattcacga acttccagac ctgcagagtt
     4441 aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacactcgct taacgctggt
     4501 attttttgcc aggaaatcgg cagctacgcg tagtgcgcca gtgccccccg gagtctgtgc
     4561 catgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca gttcctgagt
     4621 gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg tttcattttc
     4681 gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc ccgtctcatc
     4741 tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac gaaacagatc
     4801 ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca tgacgaggtt
     4861 ccattatggt tacagaaggg aagtccgcta tcagggtaac gggagattta caaaattcca
     4921 actattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc tggaaggtat
     4981 aaaaaaaaca ggaccaaagt cctgtttttt cggcatttaa caaagaggtg tgctattaga
     5041 actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt ttgttatcag
     5101 aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg aagtagtagg
     5161 ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct acgtctttcg
     5221 ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg tattgcgcaa
     5281 ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat ttattagtga
     5341 tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg ttcgcgtcgt
     5401 acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt tgagcttctt
     5461 gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct tcgtattcgt
     5521 agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca cgctcgtttt
     5581 tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag ttggagttac
     5641 gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat gcagtatcac
     5701 caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca ccgtagttac
     5761 ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc agacgcgttt
     5821 tgttaccagt ttgagcgtca gcgccttcag agttgttacc ttggaagtta tattcccact
     5881 gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca agacgggcat
     5941 aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggaa aaataatgca
     6001 gaccaacagc tttaccgtac agatctactt tgttgccatc tttgttatag atttctgcag
     6061 cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata ttgcgcttca
     6121 tcattattta ttaccctcat ggtttttttt atgacacctg ccactgccgt caataagttc
     6181 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
     6241 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctgcaaagat aaagatttag
     6301 tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggaactttgt gaacaccgtc
     6361 atatttccat agaaacgtga tgatatttac agcaattttt atctatttat atgatttact
     6421 tatatttaaa ttaactaagc ggaaattttg tttctgatgg aaactttatc gacctggcac
     6481 aaaatcctct ttcagacatc cagaatgcca aaaaatagta tgaaaattgt gctatcagct
     6541 aagaaaaaac taatccgcaa taagaatacg ggttattttt tgtgcgtaaa tgttgcaatg
     6601 taatattccg taacaggatg atcgttatgg cggggttgca aatagattgc ttgacttaac
     6661 aaacagataa cttgacagaa aggataaaaa agagccagca ggcgctggct ctttgaggaa
     6721 gaaaatcaga agctggcgtt gcgcggagta cgtgggaacg gaatcacatc acgcacgttt
     6781 tgcacgccag ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc tgaatgcgga
     6841 acagtaccgt agcgacgcag atcgcgatac caccagtaat cttctttatt caggcccatt
     6901 tccagcatac gctcgtccag aacgtccaga cgttcttcac gctgggagcc tccaatgatc
     6961 tcaccgatgc ccggagccag aacgtccata gccgcaacgg ttttaccgtc ttcgttgagg
     7021 cgcatataga acgctttaat atctttcgga tagtttttaa ccactaccgg tgctttaaag
     7081 tgttcttccg ccagataacg ctcatgctca gaagagagat cgacgcccca gtaaaccggg
     7141 ttttcaaact tcctgccgca gttttcgaga atggtcactg cgtcggtgta atccacctgc
     7201 gcgaaatcgg cctcaatgaa gcgttccaga cgtgaaacgg catctttatc tacgcgttca
     7261 gcgaagaatt tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac atatttcagc
     7321 atggcttcag ccagacccgc gatatcgttc aggttagcaa acgccacttc cggctccagc
     7381 atccagaatt ccgccaggtg acggctggtg ttggagtttt cagcacggaa agtcgggccg
     7441 aaggtataaa ttttggataa tgcgcaagcg taggtttcgc cgttcaactg gccagatacg
     7501 gtcaggaaag actctttacc aaagaagtct ttgtcgaaat ccactttgcc ctgatcgtta
     7561 cgcggcaggt tttccagatc cagcgtagaa acgcggaaca tttcgcctgc accttcggta
     7621 tcagatgcgg taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt aaagaagcga
     7681 tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg caccaatcag gtttgtgcgc
     7741 ggacgcaggt gagcgacttc acgcagatac tcaatgctgt ggcgttttgc cgccatcggg
     7801 taagtgtctg gatcttcaac ccaaccagca acttcaactt tgctggcctg aatttcaaat
     7861 tgttgcccct ggcccggcga cgccacgact ttacccgtca caatgaccga gcagccggtg
     7921 gtcagacgca ggacgtcttc attgtaattg ggcagagaat tattgatgac agcctgtaca
     7981 ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga tgccagcttt tgaatctcgg
     8041 cgggtacgta cccatccgcg tacggtgact tcgctgtcaa cggctacgcg gccctggagt
     8101 acgtcggcta caggcacaac gctcataata ttctctctgt taatagtcgg aaaaaataaa
     8161 cacttgtcca cccgtaaatg gggggatacc tatgttacct ggcatctgca atcagacaag
     8221 cagaattcgc aaatgcagcg aaagatttcg gaattaaagg taaaaaaagg gagctgatta
     8281 gctccctgat gatattaact ggctttttta atatgcggaa ggtcgaacgc tttgcgcagc
     8341 gcccgaacaa acgctttatc atggcagata gttttgccag ggctgtcaga aagtttcgcc
     8401 accggtttac cgttacactc taccaactta atcacaatat tcaggggttt tacctggggg
     8461 atatcgcagg tcaggcgagt cccaatacca aaacttaatt gcacgcggga agagaagtgg
     8521 cggtatagct caaccgcttt gcgtaaatcc agattgtcag agaaaaccag cgttttactc
     8581 tgtggatcaa ttcccagctt tttataatgt gcaatggctt tttcacccca ttcaaccggg
     8641 tcgccagagt catgacgcag gccctgatac cgactggcga actcgacacc gaaatcacgc
     8701 aggaaagcat ccatagtgat gcagtcggtt aatgcaatgc caagttggtc gggatattct
     8761 tccagccagg cagcaagtgc agctcgctgg ctgttggcta ggtccgggct gatttgctga
     8821 tgtgcctgga accattcgtg cgcctgtgtt cccatcggcg tgagggaaag ccgacgcgcc
     8881 agatcgtagt tgctggtgcc cacgaaccag gattcctgtt gcagacgctt aacgatggtt
     8941 tcttgtactt cgcgagaaaa acggcgacgg gtgccaaaat ccatcagatg gaagcgcgac
     9001 atatcaagac cggcggttaa cgccgagaag tcgactaatt tgctttccag cgtgtcgagg
     9061 gcttgcgcaa cgtcggcctg cggtgagcga tagcgatgta ccatttcact gataaccgcc
     9121 agcaaaggaa cttcccagag gatgacttca cgccacggac cgcttaagcg aatatccagc
     9181 ttgccattat cgttggacac ggtgacttgt tccgggttaa agcggaattc gcgtaaccag
     9241 ttaagatagt cggccttaaa gaaaggcagg gcagagagcc actgatattc atcatcctgc
     9301 aggcgcaggt gctgcatcgc ctgaatctgt tcacgaatag catcggcata aatacccagc
     9361 agatcgtcac ctcggcaacg aaactccgcc gcgacatgca catcgtaata gtgatgaaac
     9421 acggcttgct gcatatgcaa cttataagca tctgtatcca gcaacgagtg cagaacagga
     9481 gaagcgaatt gtgtcatagg tgcgcagtag catcctcgta caggagcgtt tagtacaata
     9541 aacatcttca ggaactgctg gagtatacct tgtttcgcaa tttattgaac cccgatcaca
     9601 ccatatgcca cctttctggt cgatggcatt acgcggtgta tgttataaaa atgtagcaat
     9661 aaaggcgttt gtacctgaaa agatgaagat tctgcatagc gcgatttacg caacaggaat
     9721 agactgaaca ccagactcta taaaagatgc taaaggttat ttatgactca acagccacaa
     9781 gccaaatacc gtcacgatta tcgtgcgccg gattaccaga ttactgatat tgacttgacc
     9841 tttgacctcg acgcgcaaaa gacggtcgtt accgcggtca gccaggctgt ccgtcatggt
     9901 gcatcagatg ctccgcttcg tctcaacggc gaagacctca aactggtttc tgttcatatt
     9961 aatgatgagc cgtggaccgc ctggaaagaa gaagagggcg cactggtcat cagtaatttg
    10021 ccggagcatt ttacgcttaa gatcgttaat gaaataagcc cggcggcgaa taccgcgctg
    10081 gaagggcttt atcagtcagg cgatgcgctt tgcacccagt gtgaagccga aggtttccgc
    10141 catattacgt attatctcga ccgcccggac gtgctggcgc gttttaccac caaaattatt
    10201 gccgataaaa ccaaatatcc cttcctgctt tccaacggta accgcgttgc gcaaggtgaa
    10261 ctggaaaacg gacgccattg ggtacagtgg caggacccgt tcccgaaacc gtgctacctg
    10321 tttgcgctgg tggcaggcga ctttgatgta ctgcgcgaca cctttaccac gcgttctggt
    10381 cgcgaagtgg cgctggagct gtacgtcgat cgcggcaacc ttgatcgcgc gccgtgggcg
    10441 atgacctcgc tgaaaaactc aatgaaatgg gatgaagaac gcttcggcct ggagtatgac
    10501 ctcgacatct atatgatcgt cgcggtggat ttcttcaata tgggcgcaat ggagaataag
    10561 ggtttgaata tctttaactc caaatatgtg ctggcccgca ccgacaccgc cactgacaaa
    10621 gattacctcg atattgaacg cgttatcggc catgaatatt tccataactg gaccggtaac
    10681 cgagtcacct gccgcgactg gttccagctc agcctgaaag aaggtttaac cgtcttccgc
    10741 gatcaggaat tcagctctga ccttggttcc cgtgcagtta accgcatcaa taatgtgcgc
    10801 accatgcgcg gattgcagtt tgcggaagac gccagcccga tggcgcaccc gatccgcccg
    10861 gacatggtca ttgagatgaa caacttctat accctgaccg tttacgagaa gggcgcggaa
    10921 gtgatccgta tgatccacac cctgctgggc gaagaaaact tccagaaagg gatgcaactc
    10981 tatttcgagc gccatgatgg cagcgcggca acctgcgacg actttgtgca ggcgatggaa
    11041 gatgcgtcga atgtcgatct ctcccatttc cgccgttggt acagccagtc cggcacgccg
    11101 attgtgaccg tcaaagacga ctacaatccg gaaaccgagc agtacaccct gaccatcagc
    11161 cagcgcacgc cagccacgcc ggatcaggca gaaaaacagc cgctgcatat tccgtttgcc
    11221 atcgaattgt atgacaacga aggcaaagtg atctcgttgc agaaaggcgg tcatccggtg
    11281 aattccgtgc tgaacgtcac ccaggcggaa cagacctttg tttttgataa tgtctacttc
    11341 cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag tgaaactgga atataagtgg
    11401 agcgatcagc aactgacctt cctgatgcgt catgcgcgta atgatttctc ccgctgggat
    11461 gcggcacaaa gcctgctggc aacctacatc aagctgaacg tcgcccgaca tcagcaaggg
    11521 cagccgctgt ctctgccggt acatgtggct gacgctttcc gcgcagtgct gctcgatgag
    11581 aagattgatc cggcgctggc ggcagaaatc ctgacgctgc cttctgtcaa tgaaatggct
    11641 gaactgttcg atatcatcga cccgattgct attgccgaag tacgcgaagc actcactcgt
    11701 actctggcga ctgaactggc ggatgagctg ctggctattt acaacgcgaa ttaccagagc
    11761 gagtaccgtg ttgagcatga agatattgcg aaacgcactc tgcgtaatgc ctgcctgcgc
    11821 ttccttgctt ttggtgaaac gcatctggct gacgtgctgg tgagcaagca gttccacgaa
    11881 gcgaacaata tgaccgatgc gctggcggcg ctttctgcgg cggttgccgc acagctgcca
    11941 tgccgtgatg cgctgatgca ggaatacgac gataagtggc atcaggacgg tctggtgatg
    12001 gataaatggt ttatcctgca agccaccagc ccggcggcga atgtgctgga gacggtgcgc
    12061 ggtctgttgc agcatcgctc atttaccatg agcaacccga accgtattcg ttcgttgatt
    12121 ggcgcgtttg cgggcagcaa tccggcagcg ttccatgccg aagatgggag tggttaccag
    12181 ttcctggtgg aaatgcttac cgacctcaac agccgtaacc cgcaggtggc ttcacgtctg
    12241 attgaaccgc tgattcgcct gaaacgttat gatgccaaac gtcaggagaa aatgcgcgcg
    12301 gcgctggaac agttgaaagg gctggaaaat ctctctggcg atctgtacga gaagatcacc
    12361 aaagcactgg cttgataaat aaccgaatgg cggcaatagc gccgccattc ggggaattta
    12421 cccctgtttt ctcaggcgaa tttcagattc accacgctgc atcacccttt gcaacacttc
    12481 cgattccagc tccgctaatc gcaccgaccc taatcgacgc ggagggggga tatccactgt
    12541 caaatccaga ccaatttttc cctcttcaat taataacacc cggtcagcca ttgctaccgc
    12601 ttcgctcaca tcatgcgtca ccaacagtac ggtaaagccg tgctcctgcc aaagtgacac
    12661 aatcaaatcc tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag
    12721 caacaataaa cccggtcgat gaatcaacgc tcttgccagc accactcgct gtttctgccc
    12781 gcctgaaagt gcggcaggcc attccccggc gcgattctcc agtcctaccg cagccaacgc
    12841 ccgacgcgcg gcatcgcgcc actggccttt aaggcctaac ccaacgttat caatcaccga
    12901 tttccacggt agcagacgcg catcctgaaa catcattcgc gtgtcttcct gaatttcagc
    12961 cagcggtgtg gtgcctgcca acacatcgcc tgcggttggc gtttccagcc cggccagcag
    13021 gcgcagcagg gtacttttgc caccaccgcc acaaactgac ctgccggaat atgtaaatcc
    13081 agttggttca ggacgatatt ttccgcgtaa tgtttgctta ctgcattgag caacaatggt
    13141 gtgccctggt tcagacgagc agtattcata ccgtggcctc cttcaaatga taagccgggt
    13201 tccagcgcaa ccagaggcgc tctaacagct gcgcgctgac gtcagcaagt ttgccgagca
    13261 gggcgtaaag aataatagcg actaccacca cgtccgtttg caaaaactct cgcgcattca
    13321 tcgccagata accaatgccg gaattggcag aaatggtttc ggcaacaatc agcgtcagcc
    13381 acatcaggcc caacgcaaaa cgcacgccga ccataattga gggcagggca ccaggcagga
    13441 tcacatggat aaacagcggt atgccggata atccatagct acgcgccatc tccaccagcc
    13501 cgcgatcgat attacggatg ccatgccagg tgttgatata aatgggaaac agcgtaccga
    13561 gcgccaccag aaagattttt gcggattcat caatgccaaa ccacaaaatc accagcggga
    13621 tcagcgccag atgcggcacg ttgcgcaaca tctgaattga ggtatccagc agccgttctc
    13681 cccagcgaga tagcccgcta atcagtccca aaatcaaccc cagcgatccg ccaattgaaa
    13741 agccaatcag cgcccgctag gaactgatcg ccagatgctg ccacagttcg ccgctggcgg
    13801 agagcgtcca gaacgccgtc accacccctt ccggtgaggg caaaatacgc gtcgacagcc
    13861 agccaactga ggaggccagt tgccacaccg ccacgatgcc caccggtaaa aactaggggg
    13921 caacgcgcaa taaccacttc ttcactggcg ttgccattat ttcttcctta gctttgcgcg
    13981 actttacggg ggataaaatc attcgccacc gcttcgcctt gcggattcag cggctgcggc
    14041 tggggaattt ccggaatggc gacatccaga tgtgggaaca gcaactcgcc gacccgatac
    14101 gcttcttcca gatgcggata gcccgaaagc acaaagctgt cgatgccaag cgcggcgtat
    14161 tcgttgattc gcgcagcgac cgtaggacca tcgcccacca gcgccgttcc ggtaccgccg
    14221 cgcactaagc caacgcccgc ccataaattg gggctgatct ccagattgtc gcgcttgccg
    14281 ttatgtaacg ccgccattcg ctgttgtcct acggaatccg tccgggcgaa tgcggcctgt
    14341 gctttggcga tagtttcatc atcaagatgc gagattaacc gctcggcggc ctgccacgct
    14401 tcgtcgttag tttcacgaac aatcacatgc agacgaatac cgaaacgaat tttgcgtcca
    14461 tgcgcggcag ctttcgcccg cacttgttcg atcttctctt ttactagttc tggcggttcg
    14521 ccccaggtga ggtagagatc aacttgttct gccgccagct cctgggcgac gtctgacgat
    14581 ccgccaaagt aaagcggagg ataaggctgt tgaatcggcg ggaagagcag ttttgctccg
    14641 cgcacatgaa tatgtttacc gttgaaattg acggtttccc ccagcaataa gcgccgccag
    14701 acctaggtaa attccgccga ggcttcgtag cgctcgctat gatcaaggaa cacgccgtcg
    14761 cctgccagct cttgtggatc gctgcctgtg accagattaa acaacgcacg accatttgag
    14821 agacggtcaa gcgtggcggc ctggcgagcg gcaacggtag gtgaggttac gctggggcgc
    14881 agggcgacaa gaaacttcag ccgctgcgtc accgggatca tcgacgccgc caccagccac
    14941 gcatcttcac acgatcgccc cgttgggatc agcacaccgg tatagcccag acgatccgcc
    15001 gcttgcgcaa tttgttgcag ataaccgtga tcaaccgggc gtgaaccttc ttccgttccc
    15061 agatagtgcc cgtcaccgtg ggtcggtaaa aaccagaaca tattcagact cataattgtt
    15121 ttccttccag ttgagtgggc tgccagatgc gctggcgaat atcgactttt ttcggcacca
    15181 gacgattttc ataaaacaga tctgccgttt gctgctgtaa ggcggcaacc tcggcgttaa
    15241 ccggtttgat ggtggtagga gggcgatgat ctaagtaaga ggcaatcacc ggtgccggta
    15301 agcccatcgt ttttgccagt aaagcgatgc tttgctcgcg ctggctgcgg gttaacgcat
    15361 cggcctcact aaaggttgcc agtacgccct gaataaaagc gccgtttttt tctgcatagg
    15421 gacgagctgc cagataaaac gatccagttt gattgagatc ggtgccgtct ttcagcaccc
    15481 gcacgccgcc ctgtaataat gcagcagagt agtagggatc ccagatagcc caggcgtcaa
    15541 cgttaccttg ctggaacgcg gcgcgggcat cagcgggcgt caggtaagtg ggttggatat
    15601 cggtaaactt aagtccggcc tgacgcagtg cacgcagtaa aaggttgtgt gaactggaac
    15661 ctttctgaaa ggcaactttg tgacctttaa gatcggctac ggttttgatc gggctgtttt
    15721 ctgccaccag aatcacttcg gctttgggct tcggtggctc gacgcccaca tataccaaat
    15781 cagccccggc agcctgggca aagattggcg gaatatcccc ggtactgccg agatcaatac
    15841 tgccaacgtt taaggcttcc aacatttgcg gacccgcggg gaactccacc caggagattt
    15901 ttgattgcgg atagcgtttt tccagtaact ggtggctttt tgccagtacc ataccaatac
    15961 tgcctttctg atagcctata cataacgctt caggcgagga ttctgcagca accgcaatcg
    16021 tagagacgct aagcaatccc accagcgcca gtttaatgat gttacgcatg ggcattacct
    16081 cgcagagaca gaaggtcagg aacctgaaca tcgcggcggt gcaatgcctg ccagaaagtt
    16141 tctagcgcgg tatcaagacg ggtttgcaga tttggcgtga actggggtct gtgatggtaa
    16201 tcaattactt gtgagtcatc ggcaaacacg ccgtgcagga tctcctgagc tttcagtgcg
    16261 cttaaaactg gtttaagggc ataatcgacc gccagcagat gggccacggt accgcccgtc
    16321 gccagcggta gcaccacttt gccttgcaaa gcgcgctctg gcagcaggtc gagcagggtt
    16381 ttcagcgcgc cggaataggc ggctttatac acaggtgtag cgactatcag tccatcagcc
    16441 tgttgcagct gttcggtgaa ggtcttgagt gccggactat cgaaacgagc ataaagcaga
    16501 tcttccggga cgaagttttg cagattccag tgataaacct ctacatccag gccatttagt
    16561 ttttcccgcg catattccag caaggagctg gagcgagaag gaaagcgagg actacccgcc
    16621 agggtgatga cacgcatact ctctccttat aaccaattgt tctttttttg ttaacattga
    16681 taacaattcg gtcagtctgt cggagagaca agaaaatgcc aaatataaat tttgtgtatc
    16741 tttttctgaa aataaactaa aga
//
LOCUS       DAJTNN010000082        16656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTNN010000082 DAJTNN010000000
VERSION     DAJTNN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.082"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1324)
                     /locus_tag="OO150_002354"
     CDS             complement(<1..1324)
                     /locus_tag="OO150_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5516476.1"
                     /translation="MAFKHYDVVRAASPSDLAKQLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSD"
     gene            complement(2300..2376)
                     /locus_tag="OO150_002355"
     tRNA            complement(2300..2376)
                     /locus_tag="OO150_002355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2340..2342),aa:Arg,seq:tct)
     gene            complement(2390..2466)
                     /locus_tag="OO150_002356"
     tRNA            complement(2390..2466)
                     /locus_tag="OO150_002356"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2430..2432),aa:Arg,seq:tcg)
     gene            complement(2474..2549)
                     /locus_tag="OO150_002357"
     tRNA            complement(2474..2549)
                     /locus_tag="OO150_002357"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2514..2516),aa:Ile,seq:cat)
     gene            complement(2599..3649)
                     /locus_tag="OO150_002358"
                     /pseudo
     CDS             complement(2599..3649)
                     /locus_tag="OO150_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(3757..3997)
                     /locus_tag="OO150_002359"
                     /pseudo
     CDS             complement(3757..3997)
                     /locus_tag="OO150_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4224..5045)
                     /locus_tag="OO150_002360"
     CDS             complement(4224..5045)
                     /locus_tag="OO150_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5516477.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            complement(5042..5422)
                     /locus_tag="OO150_002361"
     CDS             complement(5042..5422)
                     /locus_tag="OO150_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5516478.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(5423..6406)
                     /locus_tag="OO150_002362"
     CDS             complement(5423..6406)
                     /locus_tag="OO150_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5516479.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLAEDKSLLPFFS
                     DERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLGQLAHQNLFAWMIDVIRHAMNGSQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQNPPQEKTVVSI
                     AVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKA
                     DDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(6480..6752)
                     /locus_tag="OO150_002363"
     CDS             complement(6480..6752)
                     /locus_tag="OO150_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516480.1"
                     /translation="MAHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(6920..7075)
                     /gene="hokD"
                     /locus_tag="OO150_002364"
     CDS             complement(6920..7075)
                     /gene="hokD"
                     /locus_tag="OO150_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5516481.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(7313..7978)
                     /locus_tag="OO150_002365"
     CDS             complement(7313..7978)
                     /locus_tag="OO150_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5516482.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(8153..8578)
                     /locus_tag="OO150_002366"
     CDS             complement(8153..8578)
                     /locus_tag="OO150_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5516483.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRLSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            complement(8594..9364)
                     /locus_tag="OO150_002367"
     CDS             complement(8594..9364)
                     /locus_tag="OO150_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR5516484.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(9386..10128)
                     /locus_tag="OO150_002368"
                     /pseudo
     CDS             complement(9386..10128)
                     /locus_tag="OO150_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(10135..11097)
                     /locus_tag="OO150_002369"
     CDS             complement(10135..11097)
                     /locus_tag="OO150_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5516485.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(11120..11545)
                     /locus_tag="OO150_002370"
     CDS             complement(11120..11545)
                     /locus_tag="OO150_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5516486.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDPPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(11529..11852)
                     /locus_tag="OO150_002371"
     CDS             complement(11529..11852)
                     /locus_tag="OO150_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5516487.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            11977..12453
                     /locus_tag="OO150_002372"
     CDS             11977..12453
                     /locus_tag="OO150_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516488.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKKAIVAFILSGNATEPSWVDYDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            12772..12924
                     /locus_tag="OO150_002373"
     CDS             12772..12924
                     /locus_tag="OO150_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5516489.1"
                     /translation="MDTIELGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            12936..13256
                     /locus_tag="OO150_002374"
     CDS             12936..13256
                     /locus_tag="OO150_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516490.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLDLMDIIDKGFGLLMKKKGIRIEPLENYFATKSINS
                     FDSK"
     gene            complement(13234..13509)
                     /locus_tag="OO150_002375"
     CDS             complement(13234..13509)
                     /locus_tag="OO150_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516491.1"
                     /translation="MSTENDEIINSLIRQINNFDKALQHAAARSDITLLAISFLASVM
                     DKNEVVRQSLVDYIDSLQPGTFNHESFNHEKEHVKSVINSLILNQKN"
     gene            14071..14259
                     /locus_tag="OO150_002376"
     CDS             14071..14259
                     /locus_tag="OO150_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCR5516492.1"
                     /translation="METLLPNINTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            14256..14447
                     /locus_tag="OO150_002377"
     CDS             14256..14447
                     /locus_tag="OO150_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR5516493.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            14540..>16656
                     /locus_tag="OO150_002378"
     CDS             14540..>16656
                     /locus_tag="OO150_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5516494.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RTNQQHSENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQSGGGNCPDCG
                     AVMGDATYQETFDEENQVEVQENDPKEMEGAEHPHKENAGSAQDHASDSETGETADPL
                     IAMNGHHVITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDVIKRWLKQSREA"
BASE COUNT         4189 a         4425 c         3898 g         4144 t
ORIGIN      
        1 catctgacaa ctgaattccg ccattcttaa tgctccagcc ctgcgcagca gcatctccgg
       61 ctgtcggcaa cagggagatt gtgcgtacgg atgcatcttc agacggcccc gatggcgtgt
      121 cgccgccggg cgagggtttg atttccggtg ccttaccact aatgaaggct aaggtgcgac
      181 cggctacgtt cagaatagca gttgccatac gatcgggaat aatgccacga cgcgcccatg
      241 agctgaaatg cgtcgggcga tttgatgata cccagttttt gttcgttcgg gatgccgaac
      301 cgtaataacc agacccggca atatcaggat cttctgacgg gttgtttgtc ggtgtattaa
      361 ctccgctacc atcggtcata aagggaacaa aataaatctg ctgggattct ttacctttat
      421 atgcaccata taccacttca tattgcgtac cgtgttcttg tttccacgcg tatgtcgtgt
      481 cgccacaaat ccaggggact gatgccggac ttccaccgtg acactgcgct gccagcccgg
      541 caaggtcagc acggaactgc tgtaccattg caagaaatgc tgctggctgc tgggcgtaac
      601 tggcattcgt catatcgaat tccccctgca tccagcatat cgccagcaaa acgtttttcg
      661 ggtttttctg caatgctgcc ttcgtgcgga aaagcagatc ctgatataac ggcttaccca
      721 ctccccagcg agccgaatcc tgactggctc ccgtggactc gctgaatgtc ccctccgtgc
      781 cctgggtgaa tgccgaacca ccacgacagc atggtaccag caggatcccc gcattattag
      841 ggatatacgg aagcagtttt ttggcaatat gtaagccctg tccgacacag ccgtactgcc
      901 ctttgctcag gtcagcccgg ggatggttaa tcgtactcat atcctgaaca tcatgcagac
      961 aatggtcagc aggaatgatg tcgttaaata cgcatacttc accaccggga gtcactgtgt
     1021 tacgacgggc cagttgctta atgcgcggat ggggcgcatc gtatgaatcc ggaagcggaa
     1081 gcccttcacc gtaagccatg gcattggatt gcccggccag tacgatgacg tagtaccact
     1141 ccggctcagt tgcaccactg acgaccacat caccttctgc tgcaatcgcc tgcatcaggg
     1201 tataaggggt tatggccacc ggactaccaa acggctgcca gccctccttc agtttttgtg
     1261 tcagttgttt cgcaaggtct gacggcgatg ccgccctgac cacgtcatag tgtttaaatg
     1321 ccatgaatcc tcccgggcgg gataatgttg tgagtcagat aaggagcagg ctgaagtccg
     1381 gaagttacag gacaatggca gaagggagac tacagcccgc aattcgaaaa agaccgcgca
     1441 gttgcgcaga gtgattacta tggggtatta ttcgccagct gaaatattac ttcacgtttt
     1501 attgtttatt ccttgccgcc cgcgtctccc agcgcgggct ttttttgtcc ataagaaagc
     1561 ccctccggag aggggctaaa gccgcgtatc tgtatcatca tgcacatggt gccgggtgcc
     1621 tcccggtgag ttcagcccgg tgccactaaa cccgcgtcat tctcgttttg ataatcagag
     1681 attataccgt caccagtcgc ccctccgctc agggggattc accatgcgaa atttttttaa
     1741 caaatgccca gtctgacagg caactgtcaa cttactgaat tgtgagcaac atagcattta
     1801 acggggaacc tgttttctgc agtaaaaagg cccaccggag cggatgggcc tggaaggata
     1861 gcggtcatgt gatgccggtt tcccggtaac tcagcaccgg tatctgagtc aacgttttct
     1921 ctactgggtc atttccgata cgccctgcct gctgacaggc tttcatcaca tctgaaaata
     1981 tagcaccctg actgatactg tagtacctaa ggttccagaa actgtgatgt atccggcaca
     2041 gaaaagcccc tccggagagg ggctggagag tggcgctatg tgccattgca tggtgccggg
     2101 tgcctcccgg tgaattcagt accagcacct gaatccgcga ttatcccata tacctactcg
     2161 ctgattgccc ctccgcacag ggggattcac catgccagtt tcttttaaca aactccccgc
     2221 aaaccagaca acagtcaacc gcctgaattg tgagacattt aaaaaaaagc ccgcaaaagc
     2281 gagccaggga aaataagtgt ggcgcgttgt actggattcg aaccagtgac cgattgctta
     2341 gaaggcaatt gctctgtccg gctgagctaa caacgcagga tacagataat ggaccgcctt
     2401 cggggacccg aactccgcgc aaccagcttc gaaggctggc gctctttcct gatgagctaa
     2461 tggcggtatg tgatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc
     2521 gctcgctctc accactgagc taaagggccg ggagcagaat aataatggtg cgtaattaat
     2581 tctgcaatct catccgtttc aaacgattaa atcctgaact tccctgactg tctgctcaaa
     2641 acgtccggtc tccagttcaa cgccaatcgc acgacgcccg agcgccagtg cagcttttac
     2701 cgttgaacct gagcccataa aaaaaatctg caaccaggtc tcccggacga ctgcttgcgc
     2761 tgattatctg ctgcagcatt tctgccggtt tttcgcacgg atgtttcccg ggatagaact
     2821 gcaccggttt atgtgtccac acatccgtgt acggcacctg cgccgtcaca ccaaaatacc
     2881 gccgcagatg cttatattca ctctgcagct ccacatactg ccggttcagt gacgtatacg
     2941 tatccaccag ctggtggtgg ggcttttcca gttcaccgcg ctgatgtttc tcttctgcca
     3001 cccgggcaaa cagcgactgt aatttcagat aatcgctttc gttcggtagc tgccactgac
     3061 tggcactgaa ccagtgcgac accatgtttt tctttcctgt ggcatctgca atctgttttg
     3121 ccgttatccc cagggccgcg cgcgcatcac gaaagtaaga aatcagcggg gccatcacat
     3181 gctgtttcag tgcactgccc ttcgccgcat acccggcatc tttcggacga tacggcccct
     3241 gataatgttc cgcgaacaga atgcgctctg tggcggggaa atacgcccgc aggctttcct
     3301 tgttgcatcc gttccagcgt ccggacggct tcgcccagat aatatggttc agcacactga
     3361 agcgttcacg catcatgatt tcgatatcag atgccaggcg atgaccacag aacaggtaaa
     3421 gacttccggc aggtttcagc acccgccaga actgcgccag acactggtcc agccacttca
     3481 ggtaatcatc gtcgcccttc cactggttat cccagccctc aggcttcact ttaaagtacg
     3541 gcgggtccgt gactatcagg tcaacagaat tttcgggtaa cgaccggata aattccaggc
     3601 agtcggcgtt gattaactca caactggata tttttacagt attaagcatg gatcattaag
     3661 cctgtctctg ataggctcat tctgcttttg cgcaaagcag tgggcctgag gtttgcttgt
     3721 gaacccaacg catgagcaga tggctggtgg gtgcccctaa cacccaccag ccgcccattt
     3781 accacaaata aaaaagcctt cactgcggaa ggcgtctgta acaaccgaac tgatagtctg
     3841 ccagacccgc cataaccagc tgggtcagta ttaactggca gcgttcgcgt gaaaggtaag
     3901 tattctgtgc aatctccccg actgtcgccg gttcggtaac gcttaattca ttaaacacca
     3961 ctctggcggt ttctgtcata tcctgctgtt ttagcatgtc tttttccctt ttccggttaa
     4021 cgtgacacac caataactct tgtcgaaaaa gccagcaagc tgaaagacag gtattcaccg
     4081 ccaccagcgc gtttactgta ctgacgcgat ttcagtcata aaaaacccgc caggcggcgg
     4141 ggtgtaaaaa atcttctaac gtcaggcata aaacgcccat cgttagagca aatttaccac
     4201 agattcggga aaaatcaaca acactatcgc gttaccctct ttaactgccg ctccgcccat
     4261 gcctcttcaa tgtcaaaccg aaccaccaac gtatcgtaaa agcgtttcac tgattttttc
     4321 cacgtatcaa gcgtgatagc actcgtcact ttgcatatgg cattaaatgc ctccgttgat
     4381 ggtagtcttt cacagccacg accaccacaa cgctggcagt ctctgataac aggcatacca
     4441 cgttttaccg actcttcacg atgaatggcg acaccacgcc cacggcaatc cttacaggcg
     4501 gtggaaacct caccctttcc gccacactcc ggacaggcaa cttttaccac ctccctgact
     4561 tttttccatt cttcccagta agacggatac acacctttcg tacactttgc ccataccggc
     4621 ggcttaccat ccggatactg gaccttgttt gtaaaaacta cgctttcaat aaatttttcc
     4681 ccatagcaac aagggcactg ctttttactc gctgcgctgc gggcataatc ctcaaaagcg
     4741 tacgaagcca taatgcgcat cactaccggt tttatttctg ccgggagttt tcttaacgcc
     4801 gccacgcgat cacaccgact gagtgcatat tctgtcagca attctgttgc ccgctctctg
     4861 tcattcatac taatgcccat tttcccaagg aacgcagaaa accccatctc agcccgattc
     4921 tgtgtcatgc cctgcgcggc catcacatca gtgatactca gcgcatcttt cgacgttgag
     4981 gccgatgcat cagtcaggcc gggggatttt ggggagtagt atttcggtaa atcttccagt
     5041 ttcatttttt gacctgccct tcaagcatta tggggtaaat cttcaccccc agacgtccac
     5101 cagatactgg ctgagcacga acgatattga tttcatcaaa ctgctcatcg tccattagca
     5161 accccgcatg cgtcagcgca tccagcggcg ctttcagaat attgtccagg tcacggcggc
     5221 gcttatccgg tggttctgca ataattttta ttgccaacct tccggacagg cttaatttca
     5281 gccgctgctg gcgaacaata agcgccactg cccggcgata acgctccccg gcttttgata
     5341 caaaatatgt gctgccacga cgacgccagt aagtgttcac cgttggcggg taaggcaaaa
     5401 caaattctat gcgttcagtc atttatgctt tccacttcag aacacccgaa tttctcgcgt
     5461 gcattaaaaa acgaatcagc aacaacagct ggctgccgtg tttttcttca aaatctttta
     5521 ccccggcgtg tagttcgcta tggcatttac ggcacagcgg aataacaaac aaatcatcag
     5581 cctttgttcc catccctccc agtccatgac caatgatgtg atgcggatca tctgcctgat
     5641 tgccacacgt catgcatttc tgcgttttta cccaacgcgt gtatacaggc atctcttccc
     5701 gttgtgattt ctggcgctgg agatactgag ccggtgactc cggatcaacg gcaatgctga
     5761 ccaccgtctt ttcctgtggc gggttttgct ggtgggcgtg aggcagcggc gcaagatttt
     5821 ttgtgcgctg cttcagtatg ctggtggcgg tctgctctcc cggtacgatg tcgctttcac
     5881 ggtacattga gcggattttt tccgcacgca accccagcga acgacgtaat accgcttccg
     5941 gtagcgcgtc cgccacctga ttgcggaccg cccaccagga taattcagcc agcgataatt
     6001 cccgttcctg cgagccattc attgcatggc gtatgacgtc aatcatccat gcaaacaggt
     6061 tttggtgagc aagttgcccg agtgattcgg aggtctggtc gcgcagctgg ttgtcgcagt
     6121 gccagcacaa caccattgcg ccggtaccat aacggtgaat gacggtttca ctgtggtgat
     6181 aatcgccgtg tggccactgg caggatttaa catggcgcag taaccagtca gacaatgcgc
     6241 cagcgccacc agcagcacga atcactcgtt cgtcgctgaa aaatggcagt aatgatttat
     6301 cctccgccag cggctggcga acggcaggaa cgaccccgga cggcagatta cgcatgcttt
     6361 tcggttccgg ctccaccagt acccgggtat tgtggaatac cggcatggat tcacggcccg
     6421 gcttaacgat caccagcccg agttccggta ccagaacagg tcgaagtaat acccgcacgt
     6481 tacctccaga tgcgttgctg gaatgtgcgg gacggacgcg gtgggcgttc agagtaagga
     6541 agcctgacgg agattatcca gtgacgataa tcgaggctga gggctttctt aatctcgtat
     6601 ccgcgtctgc ggtagttatg aattagccat tcggcctgtt cttcagtaca tggtgggtgt
     6661 tggtaccagt cggttttaaa tgcgtgtgaa cgccgcccat gccggatggc aaggtcggta
     6721 tcagaattgt gaaatttggt tttgtgcgcc atctgttttc tctgctggcg cagcaggtgt
     6781 caggtgttca ggctgacgtg cgaattgtaa accagaatgc cagaaaaaaa caaaacccgc
     6841 cgaaccgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt ggcgagggat
     6901 ttctcccccg ccaggtctct tactcctcag gttcgtaagc tgtgaagaca gcgacctccg
     6961 tctggccggt tcggattcgt acctcgcaga ggtctttcct cgttaccagt gccgtcacta
     7021 tgacggttaa acagatgacg atcagggcga ttaacatcgc cttttgctgc ttcatagcct
     7081 gcttcccctt gcctttcggc acgtaagagg ctaacctaca tttgtgagac atagattggg
     7141 cctcagatta atgttaagcg tcttgcagga cgcgaaatgt taactggggc ttttctctat
     7201 ctgcctttca gtgttcatgc ctgagacaga tagcctcaag cacccgcagc cattctactt
     7261 aactcacgtc acctcgccaa tatgaaatca atcagaaagg tgatccataa aatcactcct
     7321 tctcttcttt tccgtagtgg agttggccaa ttttgataag agggcgtccc tgagatttgc
     7381 ggtgtagatt ggtatcgcgc agagaataca cacagccaca atattcctgc tgatagaatt
     7441 tttcgcgctt gctgatttca atcatacggg acgagccgcc ctgcttgcgc cagttataat
     7501 cccagtacac catacccgga taatgcgcaa cagctcgccg cccacactcg ttaacctgct
     7561 gcatattttt ccagcgtgaa atgcccagtg aactgctgat cacactgaaa ccattttcag
     7621 cagcgtacaa cgctgtccgc tcaaaacgca tgtcaaaaca catggtacaa cggatccccc
     7681 tctcaggctc ccattccatt cctttggcac gttcaaacca gttgtcggtg tcgtaatcag
     7741 catcgataaa cggcacgccg tgttgttcag caaagcgaat attttcatcc ttacgaatta
     7801 aatactcttt ctgaggatga atgttcgggt tgtagaaaaa gatggtgtag tcgattcccg
     7861 aggcctgaag cgcctccatc acttcaccgg aacatggagc acagcaagag tgcagtagta
     7921 gtttgtttgc cccgtttggg agctccaatt taggccgttt gaaatcagca atagtcataa
     7981 atatttttat tggggtcatg aaaatagcac agagtgtagc atcagagcag ggctatcggg
     8041 aatatatgtc taaatctggt aatatctggt tttgacgcaa agcggacaac cacgctggct
     8101 ctaccctgcg ccatgaaaat gtcaattcac atctgaacta atgctcttta atctagtaac
     8161 gtctaaaata cctaacattt ccttgataaa atgccagtac acgctgcata gcttcgctct
     8221 tccggcactc gcgacagatt atattcaggc gcctgtcgta gcggcgtatt tcgccgtctg
     8281 gtaacgacca gataaggtcc ggatcaacca ctgcaggttt cttcaccttt gcccttgaga
     8341 gttttttgcg agcattttgc cagtccttac gcgcctgttc agacgggaat aacccgtaac
     8401 cagagttgta tacatcgcca ctggcaacca gctctctggc gagaacactc atcagatatc
     8461 ttgtcgcacc tgtcttggct tccagttgcc gcaacgtctc gcgaccgctc agacgtacaa
     8521 gttcaacaac ctgcccttta attttttccc gctcttcttg tgtaaatact tttgccataa
     8581 gcgcctccgg caatcacttt tccgatacaa cacggcggga agaatcagta atctgtcgaa
     8641 caatatcccg gtgcttgttc agctcccgca gcgcggcgca gactcgctcc cacttctgaa
     8701 catcactttt cgccctgcgc agcgccaggt ttgccctgcg aagggacgga aaaatcagct
     8761 catctgcttg cgtttcggta aacgatggca acggctgcac aatgtccgcc acagtttctg
     8821 ttttaatttc ttcctgtgtt gcggcttccc ggactggtaa cgcagcacct gctggctgag
     8881 gaaaggcctt accatcactt tccgttacca gcgcggcttt cggctctgct ggtaaattat
     8941 cgcccggcat gcagtaacga aatttaccgt tctgattaac gcgtgccagc cgccccgttg
     9001 cggttaccac cgccagcgtg gaagcaacct tgcgagtact gacaccgaac ttacccgcca
     9061 gttcctcaca cgttttagcc ccatcctgac cgataaactc aatcatcatg tctgcggtaa
     9121 ctttttgttc gacctccccg gtcagcatat cctgtgcttc agattttact ggccgctctt
     9181 cggttacccg ggattcacct tcgccagcca gaaaccaggt gtgaccagtt ttatcaacga
     9241 cgccttttct tttgagttcc cacagctcgt tgacagcctc ttcacgactg attccaaggc
     9301 gagctgccac cacatgtgaa gaggcttttt tcagtgcttt cagtgcgtca gatacggttt
     9361 ccattaaaat ttcctccgga caaaattact tcacaaccct catattgctg acatttggac
     9421 gccagctatc ccagttaaac gtcacccatc gaccaccgtt catggtcatg cggtccataa
     9481 tcctctcacc aagaagcgta ctcattgcgg catgattcag gtttgttaac atcccgacac
     9541 tgcacagtga tgctgtccgg cgatcaatta tctggtgcaa taccacctgc tcgtttttcg
     9601 tctcccgctg aacgcctatt tcatccagga ccagcaaatc aaccccgcaa agctcctgta
     9661 tttttccccg gatttgccgt tgtcgtagct gtcatgcaac acgctcatga cgtcagacac
     9721 ggtgacgata atcacgctgc gccccttcac catcagccgg ttgcccatcg ccgctgcaag
     9781 gtgatttttc ccggtgccgg ttttaccgct gaacacaaaa ttcgtgcacc cggtcatcag
     9841 ttcgtcagct atggatttgg cctggctcag cgcgtatttt tgcccgtcgt tctgcacctg
     9901 ataatttgca aacgagcatt tgctgtgcag aggctggatg cccgaacgat tcaggatttt
     9961 ttccacccgc aactggcgat tctggcggtt aatctcctcg ctgcgttttc gtccttcagc
    10021 aagttgccat tcccgccact cctccaccgt ccggtacggt ggaaccgacc cctgtggtgc
    10081 aagtctgcga atacgttcaa gaaccccaac tgccgcaatg tttttcatga cacgtcaccc
    10141 cctgaatccc ggcggtattt cagtgtccgg ttcagaaatg tgattcacac aacgctgcgc
    10201 aggcgaacgc cccaggcgga taaccagttc atcccatttt tcccggagtt ttgccggact
    10261 catgatgttt tttacccaga acgaatcccg ctggagacgc ccaaacattt cacaaatttg
    10321 tctgtgagtt ctgccatcca gcatccgcat tgtgcgaacg tcattggccc atgctgtcca
    10381 gttgggttct ttcggtctag tgatctcgcc atcatagctg gccgcctgct cgtaaagact
    10441 cacgattcgt ccccagatcc actgtgcgca caccaaatct tcctgacttc cccactggcg
    10501 ttttttcgca ctgaacacaa ccgcgtcagg gtgtcgggtt aaaaaatcct gttcagccgt
    10561 ctgcgggtcc ggttgcgaag cgtccggaca agaagatctt ttatctgacg gatcaggttt
    10621 taatactgac ggatcggggt caatcatcgc ccccctaatc ggcagttttt tatcaacagt
    10681 tgatccatca aaatttgacg ggtcaaccgt tgaggggtca atatttgacg ggtcaactgt
    10741 taacgggtca ttttttgccg ggctaatttt tcttttcggt ttatatgact cacgcgccgc
    10801 cgccgcagct gcttcgagtt tttccacatt aagccgatag atattgctta cattacgccc
    10861 accgacctta cgctcttcct tcgtcagcca gccctctttc gccagttctg caatagccga
    10921 tttcactgtg gattcacttc ttgcaccgat ctgacgccgg atagtttcaa tggcaggcca
    10981 tgacacgccc tcgtcattgc tgtagtctgc aagacgggcc ataaccgcca ccctggataa
    11041 gatcatgccg gtgaaggcgc acccttccca gacaagacca tgaagcttgc tgctcataaa
    11101 acccccgaac accgtgcttt tagtgcatca ccacagcatt ccctgccggg ccgccgcgat
    11161 tcatctggtc atacaaaaca accgctgacg caacaaaatc atcgacatcc ttcaccagcc
    11221 gatccctccg ttcgacgatc tcacggtaat attcagaact gtggctgcgc atacgggcca
    11281 ccagcaaagg cggcatcgcc ttttcgatcg ccggtaacag agcctgcatt ttttcaacag
    11341 catcaggggg gtctttatcc agccaacgga aaattttctg ggtattacgg gccagggctt
    11401 ccggatggct gtcgtcgtac agttcaggga acgtcatccc cagttcgaaa taagtccggg
    11461 ctatttcagc tgcaggaact ttctcaccat cagggtatgc ccaggcattc atcgccatgc
    11521 ggatgtgctc atgtttgatt ttcatgaatc atttgcctct tgatgcttcg ggtatgatcg
    11581 ttttcgtcat ttggttgctt catcgacata ttctgcgaat aacatgacga gcgtcgtaag
    11641 tatgtccaat caacatcagg acgaagttct tcacacagga caccaccttt tgttgctcgt
    11701 tcaatcgcag gacatctctc agcaggtaac tgacgtacac ctttgatcca ttgatttacg
    11761 cttggaggag atacacctaa aagcctagcc attgctgatt gcccaccgac aacagcacaa
    11821 gctcgtttga atgaatagtt atcttttttc atcgaatgaa ctccaaaaaa cacgcaacaa
    11881 tattaggctt agcctaatga aattgtcaat aggctatgcc taatacatcg agagtaggga
    11941 ttgcctaacg cgatgcgcat aggagactat taagcaatgc ttagtggtaa agacttaggc
    12001 cgagcgatag agcaggccat taacaaaaaa attgcatcag gagccgtcaa atcaaaggcg
    12061 gaaatcgcac gtcatttcaa agtccaacca ccatcaatcc atgactggat taagaaaggt
    12121 tcgataagta aagacaaact tccagaacta tggcgtttct tttctgatgt ggttggtcca
    12181 gagcattggg ggcttaacga ataccccata ccaaccccat ccacttcaga tacaaaaagt
    12241 gaacttttag acataaacag cctttatcaa gccgcctctg atgaaaaaaa agcaattgtg
    12301 gctttcatct tatctggaaa tgctacggag cctagttggg ttgattatga cgttcgcgcc
    12361 tacattgccg caatggaaat gaaggtagct aactatctga aaaatcaaga atcaaaacgg
    12421 aaaagccaga acatcaccaa gacaggaact taaacttata tggtccgacg ggaaattcct
    12481 agttcccgtt agttaactcc tactacctct cccacaaacc atcacctatt aggttgcgcc
    12541 caaattatta ggcatagcct attgacaagt aattaggcat ttcctatagt tttcccatac
    12601 caacccatcc cgtcccacac aatacagggc aatacctcga gttaccaggc agtggtcagg
    12661 ggttaagtag ccagcccgag gcgtaagaac atgacggcag ggttcaactt taataactat
    12721 gcagcaggtt tttgttccgc taccccggcg ttaaggggaa atgaggtcag catggatact
    12781 atcgagcttg gcaacagcga atctctggta tgtggcgtgt tccccaacca ggacggtacg
    12841 ttcaccgcga tgacgtatac cagaagcaaa acgtttaaaa ctgaagctgg cgcgcgtcgc
    12901 tggttaacca gaaacactga ctgatgaggt tgacgatgga atttaaagat ttaccagtac
    12961 cattccagga aatggcatcg aatgtggttc gctctcaact ggcgactctt gacctgagta
    13021 ccgtagaaaa agaaaccatc gacaatatat ccggtaacgt acgccgagcc tttatcgggc
    13081 tgtacgaaga gaagcagctc tctgataacc aggatttaca tgaaaaatac ttcctggatc
    13141 taatggacat cattgataag gggtttggct tgttaatgaa aaagaaaggg attcgaatag
    13201 aaccccttga aaattacttt gcaacaaaaa gcattaattc ttttgattca aaataagaga
    13261 attaattaca gacttaacat gctctttctc atgattgaag ctctcatgat tgaaagtgcc
    13321 gggttgaagc gagtcgatat aatcaacaag actctgtcgt acgacttcat ttttatccat
    13381 aacagatgca agaaatgaaa ttgctaaaag agttatatca ctacgcgccg cagcatgttg
    13441 caatgcttta tcaaaattat taatctggcg tatcagggag ttaatgattt catcattttc
    13501 agtcgacatt tcaccctcct gagggttggt gattaaggag ttctccacgg gtgaggtgga
    13561 gtgcgtgcgc cggacacggg tgagcatccg gcactgacag tttactgaaa ggatatttcc
    13621 ctgaaaagtc agaccataac gcgaaagcgc ccggcgaggt agctggttca tagatagcct
    13681 gtcgttaaat tttcgtcgac cgtgcgtttc cggttgtggc aatccgcgaa atggcgcggc
    13741 ggtaagtatg gcggggttat tccttccccg ttgaggacac cgagttgtca ggttgaccat
    13801 acgcttaagt gacaaccccg ctgcaacgcc ctctgttatc aattttctgg tgacgtttgg
    13861 cggtatcagt tttactccgt gactgctctg ccgccctttt taaagtgaat tttgtgatgt
    13921 ggtgaatgcg gctgagcgca cgcggaacag ttaaaaccaa aaacagtgtt atgggtggat
    13981 tctctgtatc cggcgttaat tgttaactgg ttaacgtcac ctggaggcac caggcaccgc
    14041 atcacaaaat tcattgttga ggacgcgata atggaaacgt tattaccaaa cattaatacg
    14101 tctgaaggtt gttttgaaat tggtgtcact atcagtaacc ctgtatttac tgaagatgcc
    14161 attaacaaga gaaaacacga acgggagcta ttaaataaaa tatgcattct ttcaatgctg
    14221 gcccgtttac gtccgataca aaaaggatgc tggcaatgaa tacagcattt gcacttgttc
    14281 tgacagtttt tcttgtttcc ggagagccag ttgatattgc agtcagtgtt cacaggacaa
    14341 tgcaggagtg tgtgactgca gcaaccgaac agaaaattcc cggtaactgt tacccggtcg
    14401 ataaagttat tcaccaggat aataacgaaa tcccggcagg tctttaaaac agttccgtaa
    14461 taaatatccg gtttcattct tatatgccag caatggcagg gatttgttca cccttaaatc
    14521 tgtaatgagg taaaacaaaa tgagtaaagt ctttatttgc gccgccattc cggacgaaca
    14581 ggcaataaag gaagaaggtg ccgtcgctgt agccactgcc attgaagccg gtgatgaacg
    14641 tcgcgcccgc gcaaaatttc actggcaatt cctggaacat tatccggctg ctcaggactg
    14701 cgcttataaa tttcttgtct gcgaggataa acccggtata ccccgccctg ccctcgattc
    14761 ctgggatgct gaatatatgc aggaaaaccg ctgggatgag gagtctgctt cctttgtccc
    14821 ggttgagact gaatcagatc cgatgaacgt cacttttgac aagctggccc ctgaagtaca
    14881 gaacgctgtc atggttaagt tcgacacatg tgaaaacatc accgttgata tggttattag
    14941 cgcacaggaa ttgttgcagg aagacatggc aacattcgac ggacatatcg ttgaagcgtt
    15001 gatgaaaatg ccagaagtta acgccatgta tccggagctt aagctgcatg ccatcgggtg
    15061 ggttaagcat aaatgtaagc ctggtgccaa atggcccgaa attcaggcag agatgcgcat
    15121 ctggaaaaaa cgtcgcgaag gtgaacgcaa ggaaaccgga aaatacacgt ctgttgttga
    15181 tctcgcccgc gccagaacca atcaacagca cagtgaaaat tcaacaggaa aaatcagccc
    15241 ggtcattgct gccattcatc gcgaatacaa gcagacatgg aaaacactgg atgacgaact
    15301 ggcctacgct ctctggcctg gtgatgtgga tgccggaaac attgacggca gcatccatcg
    15361 ctgggcaaaa aatgaagtta tcgacaacga ccgcgaagac tggaagcgta tctcggcatc
    15421 aatgcgcaaa cagcctgatg cccttcgcta cgaccgccag actatttttg gccttgtccg
    15481 tgaacgtccg atcgacattc acaaagaccc tgtggcactg aacaaataca ttactgaata
    15541 cctgactaca aagggcgtgt ttgaagatga aggaagaaat cagagcgcaa ctgatactct
    15601 ctcgtcgcca gtaccagaaa ctgatgcagt ggaaacggca attccgggca acgaaaaaac
    15661 cgaatgcaaa gtggaagtcg aaccatctgt agagcgtgag gggccgttct acttcctctt
    15721 caccgacaag gatggcgaaa aatacggtcg cgcaaacaaa ctttctggtc tggaaaaagc
    15781 actagccttg ggagctacgg aaatcacaaa agaggaatac ttcgcacgta aaaacggcac
    15841 gtactcaggt tcacaacaaa atactggtgc atctgacacg accgcacaac cagagccggt
    15901 aaaagttacc gctgacgaag taaacaaaat tatgcaggca gccaatatca gccagcctga
    15961 cgccgataag ttgcttgctg cctctcgcgg agaatttgtt gcagggatta gcgacccgaa
    16021 tgatccgaaa tgggtaaagg ggattgaaac ccgcgattct gtaaaccaga accagcaaga
    16081 atcggaacag aacgaccaga aagcggaaca aaacagccca aatacgcaac aaaacgagcc
    16141 agaaacgaaa caacctgagc cagtagcgca acaggaaccg gaaaaagtct gcaccgcctg
    16201 cggtcaaagc ggtggcggca actgccctga ttgtggcgcg gtgatgggtg acgcaacata
    16261 ccaggaaaca ttcgatgaag agaatcaggt tgaagttcag gaaaatgatc cgaaggaaat
    16321 ggaaggcgct gaacatccac acaaggagaa tgctggtagc gctcaggatc acgccagcga
    16381 tagtgaaact ggcgagacgg cagatccctt aattgcgatg aacggtcatc acgttatcac
    16441 atccaccagc aggacgtgtg accatctaat gatcgacctt gaaaccatgg gaaaaaatcc
    16501 tgatgccccg attatctcaa taggtgcaat atttttcgat ccgcaaaccg gagatatggg
    16561 accggaattt agtaagacta tcgatctgga aactgctggc ggagtcattg atcgtgacgt
    16621 cattaaaagg tggctgaagc aatcacgtga agcgca
//
LOCUS       DAJTNN010000083        16620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTNN010000083 DAJTNN010000000
VERSION     DAJTNN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.083"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..243)
                     /locus_tag="OO150_002379"
     CDS             complement(<1..243)
                     /locus_tag="OO150_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5516495.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGL"
     gene            350..646
                     /locus_tag="OO150_002380"
     CDS             350..646
                     /locus_tag="OO150_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5516496.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(780..1205)
                     /gene="arsC"
                     /locus_tag="OO150_002381"
     CDS             complement(780..1205)
                     /gene="arsC"
                     /locus_tag="OO150_002381"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5516497.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1218..2507)
                     /gene="arsB"
                     /locus_tag="OO150_002382"
     CDS             complement(1218..2507)
                     /gene="arsB"
                     /locus_tag="OO150_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5516498.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2562..2915)
                     /gene="arsR"
                     /locus_tag="OO150_002383"
     CDS             complement(2562..2915)
                     /gene="arsR"
                     /locus_tag="OO150_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5516499.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            3654..3737
                     /locus_tag="OO150_002384"
     CDS             3654..3737
                     /locus_tag="OO150_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5516500.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3791..5143)
                     /gene="gorA"
                     /locus_tag="OO150_002385"
     CDS             complement(3791..5143)
                     /gene="gorA"
                     /locus_tag="OO150_002385"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5516501.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSEHLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5215..6057)
                     /gene="rlmJ"
                     /locus_tag="OO150_002386"
     CDS             complement(5215..6057)
                     /gene="rlmJ"
                     /locus_tag="OO150_002386"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5516502.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            6260..8302
                     /gene="prlC"
                     /locus_tag="OO150_002387"
     CDS             6260..8302
                     /gene="prlC"
                     /locus_tag="OO150_002387"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5516503.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8310..9062
                     /gene="rsmJ"
                     /locus_tag="OO150_002388"
     CDS             8310..9062
                     /gene="rsmJ"
                     /locus_tag="OO150_002388"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5516504.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(9111..10580)
                     /gene="dtpB"
                     /locus_tag="OO150_002389"
     CDS             complement(9111..10580)
                     /gene="dtpB"
                     /locus_tag="OO150_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR5516505.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINSVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10898..11332)
                     /gene="uspA"
                     /locus_tag="OO150_002390"
     CDS             complement(10898..11332)
                     /gene="uspA"
                     /locus_tag="OO150_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5516506.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11723..12058
                     /gene="uspB"
                     /locus_tag="OO150_002391"
     CDS             11723..12058
                     /gene="uspB"
                     /locus_tag="OO150_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5516507.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12302..13801)
                     /gene="pitA"
                     /locus_tag="OO150_002392"
     CDS             complement(12302..13801)
                     /gene="pitA"
                     /locus_tag="OO150_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5516508.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14033..15235
                     /locus_tag="OO150_002393"
     CDS             14033..15235
                     /locus_tag="OO150_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5516509.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15550..16602)
                     /locus_tag="OO150_002394"
     CDS             complement(15550..16602)
                     /locus_tag="OO150_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HCR5516510.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNILFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGYVIFGIGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILIYLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4268 a         4235 c         4315 g         3802 t
ORIGIN      
        1 caatccacgt aacatgggta ttgtcgagca actacagaac ggggtaacgg ctcccaacag
       61 agcagccagg agatagcctc tcccttttct ggcccccatc atctgctgga ttttatggtc
      121 tggcaccttt tgtcttatca ggctgacacc ggcactaatc actataaaca gcaacgaaag
      181 ctcgacggcg agaaacacaa acatctctgc ggcatcttgc agcatcgcaa gccaactact
      241 catacgtaac ctcattggat aaatctatat ttccagaata atcgaaatga aattcagatc
      301 aagatatttc tggtattatc gaaatatcaa ccagacagag gggtgtcata tgcagttgga
      361 agaagtcgct aaagcgctga aagagctagg tcacccgacc cgtttgttca tatttaaaca
      421 tcttgtcaaa gccggggaac aagggttgcc ggtcggtgag ttgcaaaagc agcttgggat
      481 acctggctcc acactcagcc accatatatc agcactggta tcggtaggac tggtcaaaca
      541 aaaccgtgag agtcggacgc tgatgtgtgt ttcgcaatac gcaatgctgg aagcgataat
      601 tgaattctta cgcgaggagt gctgcgtaaa tagcaaaatt gcctgatgcg ctacgctcta
      661 catatttcat gccatatatt gaatttgcac ggttttgtag gtcggataag acgcgttagc
      721 gtcgcatccg acataaacaa agcgcacttt accaactatc ggcagtggat atcgccgctt
      781 tatttcaggc gcttacccgc ttcatcaacc actttctcgc catcttcctt ggagaatgcg
      841 cctttttgcg catctggcag aatttccagc accacttctg aagggcggca caggcgagtt
      901 cccagcggcg tcaccacaat cgggcgatta atcagaatcg ggtgctgaag cataaagtcg
      961 attaaccgat cgtcagtaaa tttatcttcc gcaaggccca gctcctcata cggttcgacg
     1021 tttttacgca gcagcgcgcg tacggaaatc cccatatcgg caatgagttt gaccagttca
     1081 tcgcgcgttg gcggagtttc cagataatgg ataatggtcg gttctgtgcc gctgttgcgg
     1141 atcatctcca gcgtattacg cgacgtgccg caggccgggt tgtgataaat ggtaatgttg
     1201 ctcatatcag tatctcatta caaagtgaaa gagagacgta gcgccagcgc agccagcgtc
     1261 acaaacagca caggcagggt catgataatc cctgtacgga agtaatatcc ccagctgata
     1321 gtcatattct tctgcgaaag tacgtgcagc cagagtagtg tagccaggct accaattggg
     1381 gtaattttcg gtcccaaatc gcagccaatc acattggcat aaaccatcgc ttctttgata
     1441 acgccagatg ccgtgctgcc atcaatggac aacgcgccaa ccagtaccgt cggcatattg
     1501 ttcataatag aagagaggaa ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg
     1561 ccgttatccg ccagcacgtt gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc
     1621 aggccataaa ccaccagata catgccgagc gagaagatga caatttgcca gggggcaccg
     1681 cgcaggactt tacccgtatt aatcgcatga ccgcgtttag cgacgacaaa taatatcagc
     1741 gcgcccacag ctgcaatggc gctcaccgga atgccgagcg gttccaggac gaaaaatccc
     1801 accagcagaa gcagtaaaac aacccagcca gttttgaacg tagcaggatc tttgatcgct
     1861 tctgcgggag atttcagcag cgccatatcg tagttctgcg gaatatcttt gcgaaaatag
     1921 agatgtagca tcaccagcgt ggcaacaatc gcggcgatat ccaccggcac catcaccgag
     1981 gcgtattcgc gaaagccgag gccaaagaaa tcagcggaaa cgatattcac caggttggag
     2041 acaataagcg gcaggctggc ggtatcggca atgaatccgg ccgccatcac gaacgccagc
     2101 gtcgtgcctt tactgaatcc taaagccagc agcatggcga tgacaatcgg cgtcaaaata
     2161 agcgctgcgc catcattggc aaacagggcg gcaacggcag caccgagcag gacaatccag
     2221 gtaaacagca agcgaccacg accattaccc cagcgtgaga cgtgcagcgc cgcccattca
     2281 aaaaagccgg actcatccag cagcaggctg atgataatga cggcgataaa cgcagccgtc
     2341 gcgttccaga cgatattcca caccaccgga atatcacccg gatggaccac gcccgtaact
     2401 aacgctagta ctgcgccgag cgttgcactc cagccgatgc ctaaaccttt cggctgccag
     2461 ataaccaata caatggtcag gacaaagata gcgcctgcca gtaacataat gcctcctgga
     2521 taaaacacat gcgaaaatac atatgtgttt agctaaattt tttaactgca aatgttctta
     2581 ctgtctgcgg aacagttttg tcgagccagg ttgcggacaa ttgcctgaat cttttcctgt
     2641 tcacatcgcc aggcttcatc aattattttc gccgcccatg ctggaatatg cggcgataag
     2701 cggtaatgaa cccatttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     2761 gccaggtggc gggagatctt gggctgcgac tggtcgagag cagtgcagag atcgcagacg
     2821 cataactctc ccagttcgct gagcagtaaa acgatgccca gacgggtttc atcagcaaga
     2881 attttgaaca attggatggg taacagaaat gacatattgc gctcctgatt gttgcaggta
     2941 gtgtctctct tcgaagcgga taagtcaaaa acatatatga tttaacgaat gtgtaagtgc
     3001 agaggaaggt aataggtgtg aattttgagt tggctattca tttgaaagga ggtctgaatc
     3061 aggacgtaaa aaaaccgact ttgcgtcggt tttttacttt ccagccctga gttggtggct
     3121 ctggctggag tgagagagtt cttatctaac agctcaatac tattagccag tggctaacat
     3181 tgcaagaatt agcgatttct tcagctggcg gagggagcac ttcggtatat atcgcagtga
     3241 cgatttatat actttcactg ggtcatcgtc atattaagcc ttgtccgaat ccggatgtaa
     3301 aaaaccgact ttgcgtcggt tttttacttt ccagccctga gttggtggct ctgactggag
     3361 tgagatagcc atcatctaac gcatcaatgc taataccata ctgaaactat tgcaaggacg
     3421 tgctggtttt ataacctgca tgtactgtat gattatccag ttagctctga ggcattttca
     3481 ctctggcaat gcgcataaac gctttcaaag tcctggtcag aagtacgggt ggtgccgtta
     3541 actgatgctc tggccggagt gagagagttc ttatctaaca atgagacatg cgccgtgaca
     3601 ggcagtggat gagtaagcgg atgcattctc actccatcgc atggagaaaa cgggtgattg
     3661 ataaagcaat catcgttcta ggggcgttaa ttgcgctgct ggaactgatc cgctttctgc
     3721 ttcagcttct gaactgatag cggaaacgta attaagggct aagagcacac tactcttagc
     3781 cctttaacat ttaacgcatt gtcacgaact cttctgccgc cgttgggtga atggcgacgg
     3841 tattgtcgaa gtcttttttg gttgccccca tcttcagcgc caccgcgaag ccctgcaaca
     3901 tttcgtccat accaaagcca atgccgtgaa taccgacaat cttctcttcc gatccaacgc
     3961 acaccagctt catgcggcac ggctggcggt gagtggtgac ggcggtatac atcgcggtga
     4021 aagaggattt atacactttc acctgatcgt cgccatactg ctcgcgcgcc tgcggttccg
     4081 ttaaaccaac agtaccaatc ggcggatggc tgaagaccac ggtcggaatg ttgctgtaat
     4141 ccagatgctc atccggcttg ttattaaaca ggtgttcaga gagacggcga cccgctgcaa
     4201 ctgccaccgg tgtcagctcc actgcacccg tgttatcgcc caccgcgtaa ataccttcaa
     4261 tgttggtgtt ttgatattta tcgacgacga tatagccttt ttcgttggtt ttaacgccag
     4321 cggcttcgag gttgatgttg tcattggcag gctcgcgacc aatcgcccaa atcaggcaat
     4381 ccaccgtttc actgcgacca tcttccagct ccagcgtcag gctaccatcg gcatttttca
     4441 ctaccgcttt cgggatggcg ttggtgtgca gctgcgggcc ttcggcgttc atcacttcga
     4501 ccagcgtttc ggaaatcatc gggtcgaagc tgcgcagcgg cgcatgttta cgcacaaaca
     4561 gatgcgtttt cgcgccgagg ccgttaatca cgcccgccag ctcaacggcg atgtaacccg
     4621 cgccaacaac cgccacgcgc tctggcaaag caggaagggc gaagaagcca tcagaatcaa
     4681 taccgtattc cacgcccgga atatccgggt ggctcggacg accgcctgtg gcgatcagaa
     4741 tatgatcggc cgtgatggtt tcgccgttta cctccagcat tttggcatca acgaagcggg
     4801 caaagccttt gattacatca acgttatttt taccgagcac gttttcatag gaagtatgaa
     4861 tacggtcgat ataggcggta cggctggcga tcaacgtttc ccagttgaac ttgttgatgg
     4921 tggtatcgaa accataatca gggccgtaca tatggatcgc ttcacggatt tgcgccgcgt
     4981 gccacatcac ttttttcggc acgcagccga cgttcacgca ggtgccgccc agctctttgg
     5041 cttcaatcag tgcgcatttc tggccgtaca tagccgcgcg gttgatggag gctataccgc
     5101 cgctgccgcc gccgatggcg atgtaatcat agtgtttagt catgacagag tgtccttatc
     5161 gttgattacc gcgattgtag cgcgagacgt aataggtgcc agcaatggct gcaattactc
     5221 cggcacgatc cagcttgcgg tggcgtgccc ggtgcctgcc ggaaccagtt tgctgtgcag
     5281 ccacggcagc acgttattca tctgctgttc cagtttccac ggcgggttaa tcacaatcat
     5341 gccggaagcg gtcatgccac ggcgatcgct gtctggcagt accgccagtt caatttgcag
     5401 aattttgcga ataccggtcg cttccagatc gtggatcatg cgcttaattt gctgacgcag
     5461 caccaccgga taccacagtg cgtaagtacc agtggcgaaa cgtttgtaac cttctgctat
     5521 cccgctgacc accgcctgat agtcggtttt catttcatac ggcgggtcga taaggattaa
     5581 accacggcgg gaaaccggcg gcagtttggc cttaagctgc tggaaaccat cggctttttc
     5641 gacgcgcgca cggctgtctt tctgaaattc agaacgcagc aacgggtaat cgctcgggtg
     5701 cagttcggtc agttgcaggc tgtcttgttc gcgcagtagc tggcgagcaa tcaatggcga
     5761 accggggtag taacgcaact gaccgctacg gttgaagtgt tttaccacat tgatgtacgc
     5821 ctccagttct gcgggcaaat cgtcctgctg ccagatacgg gcgatgcctt cgagatattc
     5881 gccggtacgc tcggcatgtt cgctgcctaa ctgataacgc ccggcccctg cgtgggtgtc
     5941 gagatagaga aacggtttat ctttctcttt cagcgactcg atgatcaggc tctgaacggt
     6001 atgtttaagg acgtcggcgt ggttgccagc gtgaaagctg tggcgataac tgagcatggg
     6061 taaaggtgtt ccggtaagta aaaatcggcc cgtattcggg gcgcacaaaa gcgtatcagg
     6121 acagtatacc gaaaagaggc cgccgccgcg aaagcgtaac gtttctcatt gaaattcact
     6181 acacttaacc ccatgctaca cacattatgt aaagcgcctg ttgagcgctt ccttaacctc
     6241 tttaaccagg actgcgcgaa tgacgaatcc gttactgact ccctttgaat tgcctccgtt
     6301 ttctaaaatt ctcccggaac atgtcgttcc agccgtgact caggcgctga acgattgccg
     6361 cgaaaatgtg gagcgcgtag tagcgcaagg ggcaccgtac acctgggaaa atctctgcca
     6421 gccgttggcg gaagtggacg atgtgttggg gcgtatcttc tccccggtca gccacctgaa
     6481 ctcggtgaaa aatagcccgg aactgcgtga agcctacgag caaaccctgc cgctgctgtc
     6541 ggaatacagc acctgggtag ggcaacatga agggctgtat aaagcgtacc gcgacctgcg
     6601 cgatggcgat cattacgcca cgctgaacac ggcgcagaag aaagcggttg ataacgccct
     6661 gcgtgatttt gaactctctg gcataggtct gccgaaagag aaacagcagc gttacggcga
     6721 aattgcgacc cgtctttctg aactgggcaa ccagtacagc aacaacgtcc tcgatgcgac
     6781 aatgggctgg accaaactcg ttaccgacga agcggagctg gcggggatgc ctgaaagcgc
     6841 gctggctgcg gcaaaagccc aggccgaagc gaaagagctg gaaggctacc tgctgacgct
     6901 ggatatccca agctacctgc cggtaatgac ctactgcgac aaccaggcct tgcgtgaaga
     6961 gatgtatcgc gcttacagca cccgcgcctc cgatcaaggc ccgaacgccg gtaaatggga
     7021 taacagcaag gtgatggaag agatcctcgc tctgcgtcac gaactggcgc aactgctggg
     7081 ctttgaaaac tacgccttta aatcccttgc cactaaaatg gcagaaaacc cgcagcaggt
     7141 gctggatttc ttaaccgatc tggcaaaacg cgcgcgtccg caaggcgaaa aagagctggc
     7201 acaactgcgc gccttcgcca aagcggaatt tggcgtcgat gagttgcagc cgtgggatat
     7261 cgcgtactac agcgaaaaac aaaaacagca cctctacagc atcagtgacg aacagctgcg
     7321 tccgtacttc ccggaaaaca aagcggttaa cggcctgttt gaagtggtga aacgtattta
     7381 cggcatcacc gctaaagagc gtaaagatgt tgatgtctgg catccggatg tacgtttctt
     7441 cgaactgtat gacgagaaca acgaactgcg cggcagcttc tacctcgatc tgtatgcccg
     7501 tgaaaacaag cgcggcgggg cgtggatgga tgactgcgta ggccagatgc gtaaagctga
     7561 cggttctctg caaaaaccgg tcgcgtattt gacttgtaac ttcaaccgcc cggtaaatgg
     7621 taaaccggcg ctgttcactc acgacgaagt gatcaccctg ttccacgagt tcggtcacgg
     7681 cctgcaccat atgctgaccc gcatcgaaac cgctggtgtt tccggtatca gcggtgtgcc
     7741 gtgggatgcg gtcgaactgc cgagtcagtt tatggaaaac tggtgctggg agccggaggc
     7801 gctggcgttt atctctggtc actatgaaac cggcgaaccg ctgccgaaag agttgctgga
     7861 taaaatgctg gcggcgaaga actaccaggc ggcgctgttt attctgcgcc agctggagtt
     7921 cggcctgttc gatttccgcc tccatgccga gttccgcccg gatcagggtg cgaaaatcct
     7981 cgaaactctg gcagaaatca agaaactggt tgccgtagta ccgtctccat cctggggccg
     8041 tttcccgcac gctttcagcc atattttcgc cggtggttat gccgcaggtt actacagcta
     8101 cctgtgggct gacgtactgg cggcagatgc cttctcgcgc tttgaggaag agggcatttt
     8161 caaccgtgaa accgggcagt cgttcctcga caacattctg agccgtggcg gttcagaaga
     8221 gccgatggat ctgttcaaac gcttccgtgg tcgtgaaccg cagctggatg cgatgctgga
     8281 gcattacggc attaagggct gatcattcag tgaaaatctg cttaattgat gaaacaggcg
     8341 ccggagacgg cgccttatct gttctggcgg cccgctgggg gctggagcac gatgaagaca
     8401 acctgatggc gctggtgtta acgccggaac atctggaatt gcgcaagcgt gatgagccaa
     8461 aacttggcgg catctttgtt gattttgttg gcggagcgat ggcgcaccga cgcaaattcg
     8521 gcggtggtcg cggtgaggcg gtggcgaaag cggtgggcat taaaggcgat tatttgccgg
     8581 atgtggtgga cgccaccgca gggctggggc gcgatgcctt tgtactggct tcagtcggct
     8641 gccgcgtgcg gatgctggag cgtaatccag tggttgccgc gctactcgac gacggcctgg
     8701 cgcgtggtta tgcggatgcg gaaatcggcg gctggttgca ggagcggttg cagttaattc
     8761 acgcctccag cctgacggcg ctaaccgata ttaccccacg cccgcaggtg gtctatctcg
     8821 atccgatgtt cccgcataag cagaaaagcg cgctagtgaa aaaagagatg cgtgtctttc
     8881 agtcgctggt gggaccggat cttgatgccg atggattact ggagcctgca cgcttgctgg
     8941 cgaccaaacg cgtggtggta aagcgcccgg actacgcgcc accgctagcg aatgtcacca
     9001 cgccaaacgc ggttgtgact aaagggcatc gctttgatat ctatgcaggt acgcccgtgt
     9061 aaaagtaaag cagggtagcg ataacgctac cctgctttct gcaagaataa ttaatggctt
     9121 tccggcgtcg caatcatgcg tttcagccac ggcaccatca acagcatcac tactgcaacg
     9181 cccagcgtga ccagaccaat cttaccaaac acgttggtat agacgggcaa cgtctcaagc
     9241 ggatcggtaa tgttgtccgg caccgcggta aatgttgcca catagccgcc cagcaagaac
     9301 gcggcagcct gcgtcaggaa ccacatcccg agaataaagc ccatcaaatg ctgcggcacc
     9361 agggcagcaa tcatcgccag gccaagggcg ctaataaaca gttcacctaa gctctggaat
     9421 aagtacacca gcacgataaa ccatggcgat gtcagccctt gtgcatccgc aaaccacatt
     9481 cccgcagctg ccgccgtcaa aaagcccagt gagcacataa acatgccgag agtaaatttc
     9541 atcggcatcg agaggtcttt gcctttgtta cccagatgcg tgtaaatgcc tgccagtatt
     9601 gggctggcga gtaccaccca gaacgggtta agcgcctgga agctgaccga gttgatggaa
     9661 aaaccgagaa tttcatgatg cacgttgttg atggcaaaga agttcagcga tgttggcatc
     9721 tgggcgtaga gaatgtaaaa caccaccgct tcgagcatca ggacaaaggc gacaaacatt
     9781 ttattgcgcc cggttttatc cagcttgaat gcctgacgaa agaagatgat ggtgacgacg
     9841 atggagagaa caatcagcac cagattggcg acttctacgt tgtgcatcag ccatgcacat
     9901 acgaagatca tcaccacgct gccaagtaat acgtacaaca gtttgctgaa gctcatcggc
     9961 ttgaagtcgg gttcagaacc aatgtctttc accattccac gacaggcgat gtaaaccagt
    10021 aatgcgataa ttaaccccgc cccgcacagg ttgtaggtga ctgaataacc gaatctatca
    10081 gcgatcacag gggccagcga taacgctatc aacgagccga tattgatcga catatagaac
    10141 agggtgaatg cgccatcaag ccgcggatct ttcggcggat agcacttcga aagcaagctg
    10201 gctgggttag ctttaaacag gccgttaccg acagcgatag tccccagggc gatgaaaatc
    10261 aggtcaggct taagtagcga catgccggtc atgaagtagc caatcgccag cacaagtgct
    10321 ccgagaacaa tggtgcgttt ggtccccagc aggtggtcgc cgacatagcc gccaatggaa
    10381 atgaggccat agaccagcgc agcaaaagca ccaaaagtga caaaagcctg ctcttgcgag
    10441 aatccaagct gtttaacgaa gaaaaccgcc agtacgccct gcacgccgta gtagccgaat
    10501 cgctcccata actcgacaaa aaagatcatg aaaaatgggc gaggttgctg cagcatcccc
    10561 atgggtgttg ttgtattcat atttattaac cttccaatac catcctgaaa agtatgaacg
    10621 cgtaagggat aacgcgcgaa aaagtttaca tctgtcagag attcttaata ttgatgcggt
    10681 gaatggatta atacactatt acaacagaaa ataaccagct aatcacacta atgatggaaa
    10741 atatgatggt gttaaatgtt gtattggcag gagattttga ttgcgtacgc agatcgtagg
    10801 ccagataagg ggtttacgct gcatcaggta acaaggcgcg ccaaaaagca aaacgcccgc
    10861 atatgcgggc gttcaggaac acgtcgtgga gggaagatta ttcttcttcg tcgcgcagcg
    10921 gaacaatcag catatcaacg tgaacggtgt tgatcagctg acgtgcggaa gacatcagtt
    10981 tgctccagaa gtcctggtgg tgaccacaaa ccaccagatc catatcgtat ttcttgattg
    11041 catcgaccag aacctggccc aggtcgccgc tgccgctcag ggtttcagtg attgggtagc
    11101 ctgcattagt ggaaagctcg gtcagcgcat gatgtgtctc ttcagagatg cgtttctgca
    11161 tatcacccag attcacatca ataagcccgg tgtataggtc agagtagttt acatctacgt
    11221 ggatcagaga aactttcgca ttgtaggggc gagccataga gactgctttc tctaccagaa
    11281 ctttgctttc cggggagaga tcgaccgcga tgagaatgtg tttataagcc atagtgttac
    11341 tccttccata aagttgtcga tgactggcca gctagcgttt cttgtgccgt tacgtcaccc
    11401 gcgtcctgcg aactacgtgc gcggggctaa cccagccgta aagattcagt gttaagacca
    11461 tccttacctt atagcgaccc ggatgatccg tcaatccgcc ttgcttaccc gttagataaa
    11521 caaattttcg acatgaatat attgatagtg gttaaccttc tggaaaaaaa acaacctgat
    11581 ctcctacact atctatagag ccgctcggat gttggtcacc acaaaagcgg tttctccagg
    11641 acctggtgtt aactgattgg cagcgtatcg ggagcggtag ccccggggtg gaactccgcg
    11701 ggcaggtcgc cggggaggag gtatgataag caccgtcgca ttattttggg ctttatgtgt
    11761 cgtttgcatt gttaacatgg cgcgctattt ctcatcacta cgcgcgttgt tagtggtact
    11821 gcgtaactgc gatccattgc tctatcaata tgttgatgga gggggctttt ttacctcaca
    11881 tggccaaccc aacaaacagg tgcgtctcgt ttggtatatc tatgcccaac gttatcgcga
    11941 tcatcacgat gatgagttta ttcgccgctg tgagcgggtg cgtcggcagt ttattctgac
    12001 tagcgcattg tgtggtctgg tggtggtcag cctgattgca ttgatgattt ggcattaagc
    12061 acttcagcaa taataacgca taaaaaaagc tggccagtaa actgacccgc tcgagactga
    12121 ttacaaacgc aaaattgcct gatgcgctac gcttatcagg cctgcataat ctctgcaatc
    12181 tattgaattt gcatgttttt gtaggccgaa taaggcgttc acgccgcatc aggcatgaac
    12241 aaagcgcact atgtcacaat ctgaagcggg ccagctgacc cgctcgtttt ggtgcgtacg
    12301 attacaggaa ctgcaaggag agccagtaca gcccgccgga aagcagtacc gcagccggaa
    12361 gggtaaacac ccaggccatc agaatgctgg tcacggtttt acgctgtaag ccgccaccat
    12421 ctaccaccat cgtccccgcg acagaagagg agagtacgtg agtagtggaa accggcatcc
    12481 cggtataact cgccaggccg atagacactg ccgccgtcat ctgggcagac atcccctgag
    12541 cgtaggtcat gcctttctta ccgattttct caccgatagt cgttgccaca cgacgccagc
    12601 caatcatcgt accgatacct aacgccagcg cgaccgccat gatgatccac accggtgcat
    12661 actcgatggt gctaagcatg tcggacttca gtttcttcaa caggcgctga tcgtcagcac
    12721 tcacgccagg tatcttcact actttgtcga tagtgtcaga aacgcacagc ataatgcggc
    12781 gcatctggct acgttgatca agcgacagct tgtcgtagct ttccacatcg gtagttaaca
    12841 tacctttcag gcggttgagc gcgttaatgg tattcgacgg atggcaatgg aactccgcgg
    12901 gttgcgttgc gccagcttcc ggagccggta ctaactgatc agcaccggta gcctgtttca
    12961 gcagcgcagg atgctgctca aagtaagctt cgacgttgtt gatggcatca cgggtacggg
    13021 tgatttcgta gccagtggca ttcatattca ccacgaagcc tgctggtgcg acaccaatca
    13081 ataccaacat aaccagacca atgcctttct gaccatcgtt cgcgccgtgc gaaaacgcca
    13141 cgccgatagc ggaaaggatc agcgcaatac gcgtccagaa cggcggcttt ttcttgccgt
    13201 ctttcttttc acgctccgct ggggtcaggt ggatacgggc gcgtttcttg gtgccgctcc
    13261 agtagcgacg cagcaagaaa atcagaccgc cagcaaacac caggccgaca ataggggaaa
    13321 caatcagaga accgaaaata cttaatactt tcgggatatt gagtgcatcc accactgacg
    13381 tcccggtcat caacgcattg gttaaaccaa tcccgatgat cgcgccaatc agtgtatgag
    13441 agctggatgc aggtaaacca aagtaccagg tacccaggtt ccagataatc gccgccagca
    13501 acatagagaa caccatggca aggccatgag acgatcccat attaagcagc agatccgtcg
    13561 gcagcatatg cacaatggca taggcaacac tcagaccacc cagcaaaaca cccaaaaagt
    13621 tgaacaccgc cgccataacc acggcgagct gagaacgcat cgcgcgggta tagataacgg
    13681 ttgccacggc gttggctgtg tcatggaaac cattgatggc ttcgtagaac agcacaaaag
    13741 ccagtgcaag caataataac agcccggtat gcaaatccag gccagcaaac aaatgtagca
    13801 taggacgtta cgccattttg aggacatgaa cgcggcgcat tatcagtgac tttcgcggcg
    13861 cgggcaaagt gaaatataga ctttttttga tttgcctcct gtatggattt cactcaaaaa
    13921 ataattatct tatataattc aggcaaatac ttccttttag taatattgat gctggtgtga
    13981 ccactgagga atctttacaa ttcacgctcg ttttttctaa gaggagcgca acgtggaaag
    14041 gtttgatgcc attattatag gcgctggtgc ggcgggtatg ttctgttctg cgctggcagg
    14101 tcaggcagga cgccgggttc tgctgatcga taatggtaaa aaaccagggc gcaaaatcct
    14161 tatgtctggc ggtgggcgct gcaactttac caacctttat gtcgaaccag gcgcttatct
    14221 gagccagaat ccgcattttt gtaagtctgc actcgcgcgt tttacccagt gggatttcat
    14281 tgatctggtc aataaacacg gcatcgcctg gcacgagaaa acgttaggac aactcttctg
    14341 cgatgactcc gcgcagcaga ttgtcgacat gctggtggat gagtgcgaga agggcaatgt
    14401 aaccttcaga ttgcgtagcg aagtgctgag tgtggcgaag gatgaaacag gcttcacgct
    14461 tgaactgaac ggcatgactg tcggttgcga aaagctggtc atcgcgaccg gtgggctgtc
    14521 aatgccgggg ctgggcgcat cgccgtttgg ttataagatt gccgaacaat ttggcctcaa
    14581 cgtgctgccg acccgtgcgg gtctggtgcc attcactctg cataaaccgt tgctcgaaga
    14641 gttacaggtg ctggcgggcg tggcggtgcc ttccgtgatt accgctgaaa acggcaccgt
    14701 tttccgtgag aacttactct tcacccaccg cggcttatct ggaccggcgg tgttgcagat
    14761 ttccagctac tggcaaccgg gtgaatttgt cagtatcaat ctgctaccgg atgtggacct
    14821 cgaaaccttc ctgaatgagc agcgtaacgc acatccgaat cagagcctga aaaacacact
    14881 ggcggttcat ctaccgaagc ggttggttga acgcttacag caactcgggc aaatcccgga
    14941 tgtttcgcta aaacagctca acgtgcgtga ccaacaggca ctgattagca cattgaccga
    15001 ctggcgcgta caacccaacg gcactgaagg ctatcgcact gccgaagtga cgctcggcgg
    15061 cgtggacacc aacgaactct cttcacggac gatggaagcg cgcaaagtgc ctgggctgta
    15121 cttcatcggc gaagtgatgg acgttaccgg ctggctgggg ggctataact tccagtgggc
    15181 gtggtcgagt gcgtgggctt gtgcgcagga tttgattgca gcgaagtcgt cctgacctgt
    15241 tacctaaaga tgaaaattat tagagccgca ttaaaaatga gctaattttg atagtggcta
    15301 tcttgtgatt attttctaat gagcccgtga actgaaaccc tccaggctta atataaggtg
    15361 gaaggaaaga tgattgaaaa ctcacccagt ggccagatct ttataaaata tgaacaggct
    15421 gaaaaagaca gtaactttgt tgttttttat ccttaaccac tatgcattaa tgctgcgtta
    15481 tttcatgatg cctaagaaaa accagcgtta cgcaaatggt caacgctggt tttatccggt
    15541 acgttgcaat tattttttag cagaacctgc ttctaatatt gaaacgattg agaacaacgt
    15601 aaagcatacc gctcccaaac cgaccaaaac ccgcgaagga ataaaatatc ccatgtcggt
    15661 ctgcgccatt tccgcaagaa acgatgccag gaaaaggcaa aacaggcagg tgaagacggg
    15721 aatcatcggt attctgttgg ctaacgaaca ggtacgtctc cataccagtg ccagtagcca
    15781 gacttttgag aatatgctgt aacagatcat tccgaggtaa ataagaataa tgccgggagc
    15841 cagtcgggcg cttgcatcgg aactgactaa gacgtatatc ccctgcagta ccgtgattga
    15901 gccgagaaaa ataacccaat aacaccacag ccagtgttct ttagttgaaa acgtattacg
    15961 tgtttgatgg acaattgtgg caacaaggcc aattagacag gcacagattg cggttaaccc
    16021 aagcaatacg tgacccgcga cataatgcgg agttatgtca gcactacgta acagcgtaat
    16081 agaccaaatg aaaccgagca gggtgagtaa aacgggaacg gcaatgaggc agttaccgat
    16141 taatgaagaa taagcctgta ccggtgttcc gtcgctcttg ctccccgctg catttttggg
    16201 aatgagcaga aagtgaccgg acgacgctgc caccgtcgat acacatgcgg caatcatacc
    16261 aatgccgaaa ataacatagc cggcgacaaa ctcgtctgcc atcacatcat tgcctgctaa
    16321 cagtgcccaa ccccatatca tggtgataat tgaccctgca tagccaataa tggggaacaa
    16381 tatattgtaa aacgtattaa cgccgcgcgt gagctgcgaa atgataataa atgcggtagt
    16441 gaataatgcc aggcatatgg ctgccagaga aatcagcaca tgacccgcga cgaaatactc
    16501 gctttgccct gaactttcca gcacaaagcc acctaacgcg atacaaatta agcccataat
    16561 caaagggatt aatttgaaaa gccacccgat atatatgttc actttaaact cctcaggtat
//
LOCUS       DAJTNN010000084        16566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTNN010000084 DAJTNN010000000
VERSION     DAJTNN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.084"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            148..567
                     /locus_tag="OO150_002395"
     CDS             148..567
                     /locus_tag="OO150_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5516511.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            567..1834
                     /gene="umuC"
                     /locus_tag="OO150_002396"
                     /pseudo
     CDS             567..1834
                     /gene="umuC"
                     /locus_tag="OO150_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(1880..2410)
                     /gene="dsbB"
                     /locus_tag="OO150_002397"
     CDS             complement(1880..2410)
                     /gene="dsbB"
                     /locus_tag="OO150_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5516512.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(2556..4097)
                     /gene="nhaB"
                     /locus_tag="OO150_002398"
     CDS             complement(2556..4097)
                     /gene="nhaB"
                     /locus_tag="OO150_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5516513.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4319..5038
                     /gene="fadR"
                     /locus_tag="OO150_002399"
     CDS             4319..5038
                     /gene="fadR"
                     /locus_tag="OO150_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5516514.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5090..6622)
                     /locus_tag="OO150_002400"
     CDS             complement(5090..6622)
                     /locus_tag="OO150_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5516515.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAKARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            6952..8250
                     /gene="dadA"
                     /locus_tag="OO150_002401"
     CDS             6952..8250
                     /gene="dadA"
                     /locus_tag="OO150_002401"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5516516.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8260..9319
                     /gene="dadX"
                     /locus_tag="OO150_002402"
                     /pseudo
     CDS             8260..9319
                     /gene="dadX"
                     /locus_tag="OO150_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(9706..11442)
                     /locus_tag="OO150_002403"
     CDS             complement(9706..11442)
                     /locus_tag="OO150_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5516517.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGTAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11537..12451)
                     /gene="ldcA"
                     /locus_tag="OO150_002404"
     CDS             complement(11537..12451)
                     /gene="ldcA"
                     /locus_tag="OO150_002404"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5516518.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVSAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12551..13162
                     /gene="emtA"
                     /locus_tag="OO150_002405"
     CDS             12551..13162
                     /gene="emtA"
                     /locus_tag="OO150_002405"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5516519.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13164..13898)
                     /locus_tag="OO150_002406"
     CDS             complement(13164..13898)
                     /locus_tag="OO150_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5516520.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14099..14353
                     /locus_tag="OO150_002407"
     CDS             14099..14353
                     /locus_tag="OO150_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5516521.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGLFRLLRRE"
     gene            14532..14972
                     /locus_tag="OO150_002408"
     CDS             14532..14972
                     /locus_tag="OO150_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516522.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPAV"
     gene            complement(15051..16566)
                     /locus_tag="OO150_002409"
                     /pseudo
     CDS             complement(15051..16566)
                     /locus_tag="OO150_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT         4160 a         4121 c         4331 g         3954 t
ORIGIN      
        1 cgccagaagg ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatacagcga
       61 caaatattga tagcctgaat cagtattgat ctgctggcaa gaatagacta ctgtatataa
      121 aaacagtata acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa
      181 attgtgactt ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca
      241 gattacgtcg aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact
      301 tacttcgtca aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta
      361 ctgattgtcg atagcgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac
      421 ggcgagttta cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac
      481 agtgcgtact cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg
      541 atccacgtcg ttaaggcgat gcgctgatgt ttgctctctg tgatgtaaac gcgttttatg
      601 ccagctgtga gacggtgttt cgccctgatt tatggggtaa accggtggtt gtactatcga
      661 ataatgacgg ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggt gttaaaatgg
      721 gcgatccctg gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca
      781 gcaattatga gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat
      841 cgccccgcgt cgagatttac agtattgatg aggcattctg cgatctgaca ggtgtgcgta
      901 attgtcgcga tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc
      961 atcttactgt tggtgtgggg atcgcccaga ccaaaacgct ggctaagctt gccaatcatg
     1021 cggcaaaaaa atggcagcgg cagacggggg ggtggtggat ttatcaaatc tggaacgcca
     1081 gcgtaaatta atgtctgctc tccccgtgga tgacgtctgg gggattggac ggcggatcag
     1141 caaaaaactg gacgcgatgg ggatcaaaac cgttcttgat ttggcggata cagatatccg
     1201 gtttatccgt aaacatttta atgtcgtgct cgaaagaacg gtgcgtgaac tgcgcggtga
     1261 accctgtttg caactggaag aatttgcacc gacaaagcag gaaattatct gttcccgctc
     1321 gtttggtgaa cgtatcacgg attatacgtc gatgcggcag gccatttgta gttacgctgc
     1381 ccgggcggcg gaaaaacttc gcagtgagca tcaatattgt cggtttatct ccacatttat
     1441 taagacgtca ccatttgcgc tcaatgaacc ttattacggc aatagcgcgt cggtaaaact
     1501 gctgacgccc actcaggaca gcagggatat cattaacgcc gctacgcgat ctctggatgc
     1561 catctggcaa gcgggccatc gctaccaaaa agcgggcgtg atgctggggg atttcttcag
     1621 tcagggagtc gcgcagctca atttattcga tgacaacgca ccgcgccccg ggagtgagca
     1681 attgatgacg gtaatggata cactgaatgc taaagagggc agaggaacac tctattttgc
     1741 cgggcagggg atccaacaac aatggcagat gaagcgagcc atgctttcac cacgttatac
     1801 aacgcgaagt tctgatttac tgcgggtcaa ataaatatag cggcaggaaa aaagtgctcc
     1861 cgcaggagcg ccgaatggat tagcgaccga acagatcacg ttttttcgct ttaaacggct
     1921 gggaaatcat caccagcacc gcgacaatca ggtaagcgat aaaaataccg agcagccact
     1981 gcggcatttc cagacctaaa aaatcccact gacgctcggc gcaatcgcca gaggcgacaa
     2041 acacttgcgg cacccattta tccagcggca gccattccgg gaaacgaacc ataaaatcac
     2101 aggtggcaaa cggcgaagga tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac
     2161 cgcggaacgc actatacaac cagataacca tcgctacata acgcagcgga gttttcgggg
     2221 cgatcgcgcc aatcagcgca gcacccagaa cgccgaataa cgcgcagcgt tcataaatac
     2281 agagcacgca aggtttcagt aacatcacat gctggaacca cagcgccgtc agttccagtg
     2341 ccagagcagt aaacgccatc aacagccacg cgccccggcc ttgtgaacat tggttcaaaa
     2401 atcgcaacat aatcatttcc ctgcaatatg catagagtgc gcagtttaaa ccaattcatt
     2461 cgctgcgcca ccaggaggca gcaaaaatag gacgcaattg gacaattatc gggcgaaaag
     2521 gcaaaccggg catttatatg cccggtaagt tgttatcaaa gcgttgctat ccagcccatt
     2581 tgcataaacc attcggttac aggggcaagc gtaaactcga cgcagagcag cccgacgagt
     2641 gtcaggacga gggtgtaagg cagggccatc cacaccatgc ggccataaga gaggcgaatc
     2701 aatggcgcga gtgcagaggt cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg
     2761 acagagggca gattggtacc ggtattaatg gcgaccgcca gcagctcgta ttgcttcaac
     2821 gtgatagcgc cactttccat tgccgctttc gcttcgttga tataaatcgt ccccacgaag
     2881 acgttatccg aaatggatga cagcagaccg ttgaaaatat agaacaacga cagctgagca
     2941 tgttccgatg cctgcaacac aaactgaata atcggcgaaa acagttgttg gtcgataatc
     3001 accgcgacta ccgagaaaaa caccgtcaac agtgcggtga atggcagaga ttcggtgaag
     3061 gctttaccga tagcatgttc atcggtgaca ccggtcaatg atgtcgccag aataatgacc
     3121 gacaaaccaa tcaagccaac ctccgccaga tgcaacgcca gcgcagtcac cagccagacg
     3181 ccgataatcg cctggacaat cagacggatt ttatcctgac gggtacgctg gtggcggctt
     3241 tgatcgtcaa actgttgcaa cacttcgcgg actttctccg gcagcgtttc accgtagcca
     3301 aaccaacgca gcttctctac cagcaggcag gttaacaggc cacaaatcag aaccggaacg
     3361 gtcaccggcg acatgcgcag gaagaaatcg ccaaaatgcc agccagccgc tttagcgatg
     3421 atcaggttct gtggttcgcc caccatggtc attacgccgc ctaatgcggt gccgacaccg
     3481 gcatgcatca tcaggctacg cagaaagcca cggaactgtt ccagaaccac tttgtaatgc
     3541 ttgtcgatat gactatcgtc ttgcaggtcg gtatcttcag tacgggaaga ggctacgcgg
     3601 tgataaatac cataaaaacc gactgcaacg ctgatcacca ccgccacgac ggttaaggca
     3661 tcgaggaacg cggagaggaa cgccgccgcc acgcaaaaag agagcgacag cagcattttg
     3721 gaacgaatgc ttaacagcaa acgggtaaat atgaacagca atagctgttt cataaaatag
     3781 atacccgcca ccataaacat cagtaacagc aagacttcaa gatttgccgc cacctcttca
     3841 cggacgtgtt ccgcgctggt catgccgatg aataccgctt caatagccaa cagaccaccg
     3901 gggagtagcg ggtagcattt cagggccatc gccagagtga aaataaattc cgctaccagc
     3961 aaccagccag cgacgaaagg gctgatgagg aaaattaacg gatttacgat taagaaaatg
     4021 atgagggcga gtttgtacca gtcgggggac tggcccaaaa aattgcgcca tagcgcgcgg
     4081 ccccaggaga tctccatgat ggtttccctt accttacaaa taatcaatga tgtttttatg
     4141 tttaaacgca aagcttaacg gtcaggcagg agtgaggcaa gtcttgatag tcaaggggaa
     4201 agagatgcgg aaaatgaagc cttgatccct tttttcttct ttttgtctgc tatcagcgta
     4261 gttagccctc tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat
     4321 ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg
     4381 gaataaccgc ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat
     4441 tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt
     4501 gaccattcaa catggcaagc cgacgaaggt gaataatttc tgggaaacct ctggtttaaa
     4561 tatccttgaa acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt
     4621 gctgtcggtg cgtaccaata tttccactat ttttattcgc accgcgtttc gtcagcatcc
     4681 cgataaagcg caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgatgcctt
     4741 tgccgagctg gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta
     4801 cggtctgatc cttaacggga tgaaagggct gtatacgcgc attggtcgtc actatttcgc
     4861 caatccggaa gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag
     4921 tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga
     4981 gatttggcac cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc
     5041 ccttccgttt aaaaagcaaa cccctcaaac gaggggtttt ttgttgtttt tacagatttc
     5101 ccattcttgg cgggcaacgt tccagcaact cgacgctgcc atcttcgttt tgctgttcca
     5161 gcatcacatc aaatccccac aggcgatgca catgcttcag cacttctttg cgcccccgat
     5221 ccagcggtgc gcgattatgt ggaatgtaac gcagcgtcag cgaacggtcg ccgcgcaaat
     5281 ccacgttcca gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc
     5341 ggttacggat ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat
     5401 gccgatcgtc atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa
     5461 actggctgat aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc
     5521 agtcggaacc ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa
     5581 tccgtttaat atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc
     5641 acgggctgtt ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca
     5701 taaaacgttc cgttactttc ccttcatcat acagatggtt aaggatggtg tagtgccaga
     5761 aggtggccca gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca
     5821 ccttacgcac aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct
     5881 ccataaaata gagcaggttt tcttgtggtt cggacggata gcggcgcgct ttagcaaccg
     5941 ttttctcttc ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat
     6001 actcttcgcg acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt
     6061 tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt
     6121 catccacgcc ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat
     6181 cgacaatcga actggcgtcg gtccaactgc ggaataagta attgttttta aagaaagagt
     6241 tatgcccata gcaggcgtgt gccatcacca gcgcttgcat ggtaatggtg ttctcttcca
     6301 tcaggtaagc gatacacggg ttagagttaa tgacgatttc ataggccagt ccttgctgac
     6361 cgtgcttata cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt
     6421 taattggcat gccgacgctg gagtaggcat ccatcatctg ttctgaggtt atcacttcaa
     6481 tctggtgcgg gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat
     6541 aaacatccag caggtcgaac gtccagtcgg gtccatcgct caaccgtgtg gtgtccttat
     6601 tcatagaatc gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag
     6661 cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt
     6721 tgcgatttct tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat
     6781 aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg
     6841 tgagccagct caccataaaa aagctgtatg ttgaataata ttttcaactg agttatcaag
     6901 atgtaattag attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt
     6961 gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga
     7021 catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag cgccgcgaat
     7081 gccgggcaaa tctcccccgg atatgctgca ccgtgggcgg caccaggtgt gcctttaaaa
     7141 gcgatcaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag
     7201 tttcagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa
     7261 aacaaagggc ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc
     7321 gccgaaacca atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc
     7381 gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg
     7441 tatcagctgc tggaatccag ccgcctggcg gaagtggagc cagcgctggc agaagtagcg
     7501 cacaaactga cgggtggtct gcaactgccc aatgatgaaa ccggagactg ccagctgttt
     7561 acccagaatc tggcgcggat ggcagagcag gcgggggtta aattccgctt taatacgccc
     7621 gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtgtgg cgatgaagtg
     7681 attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc
     7741 attgttgata ttccggtgta tccgctgaaa ggctactcgc tgactattcc aattgcgcag
     7801 gaagatggtg cgccagtatc caccattctt gatgaaacct acaaaatcgc cattacccgt
     7861 ttcgataacc gcattcgtgt tggcggaatg gcggaaattg ttggttttaa caccgagctg
     7921 ttgcaaccgc gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc acgcggcggt
     7981 catgtcgagc aggcgacttt ctggacgggt ttgcgcccga tgacgccaga cggtacgccg
     8041 gttgtcgggc gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc
     8101 tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca
     8161 gcgatcccat atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt
     8221 ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc
     8281 agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac
     8341 gcgggtggta aaagcgaacg cttacgggca tggtattgag cgtatctgga gcgcgctcgg
     8401 ggccaccgat ggctttgcat tacttaacct ggaagaggca ataacgttac gtgagcgcgg
     8461 ctggaagggg ccgatcctga tgctggaagg atttttccat gctcaggatc tggagattta
     8521 tgaccagcac cgcctgacca cctgcgtaca cagcaactgg cagctcaaag cactgcaaaa
     8581 tgcgcggcta aaagcaccgt tggatattta tcttaaagtg aacagtggga tgaatcggtt
     8641 gggcttccag cccgatcgcg tgcttaccgt ctggcagcag ttgcgggcaa tggcgaatgt
     8701 tggcgagatg accctgatgt cgcattttgc cgaagcggaa catcctgatg gaatttccag
     8761 cgcgatggcg cgtattgagc aggcggcgga agggctggag tgtcggcgtt cgttgtccaa
     8821 ttcggcggcg actctgtggc acccggaagc gcattttgac tgggttcggc ctggcattat
     8881 tttgtatggc gcttcgccgt ccggtcagtg gcgtgatatc gccaataccg gattacgtcc
     8941 ggtgatgacg ctaagcagtg agattattgg tgtccagacg ctaaaagcgg gtgagcgtgt
     9001 gggctacggc ggtcgctata ctgcgcgcga tgaacagcga atcggcattg tcgccgcagg
     9061 gtacgccgac ggttatccgc gccacgcgcc taccggtacc cctgttttag tggacggcgt
     9121 gcgcaccatg acggtgggga ccgtctcgat ggatatgctg gcggtcgatt taacgccttg
     9181 cccgcaggcg ggtattggta cgccggttga gctgtggggc aaggagatca aaattgatga
     9241 tgtcgccgcc gctgccggaa cggtgggcta tgagttgatg tgcgcgctgg cgctacgcgt
     9301 cccggttgtg acggtgtaac ttgttgtaag ccggatcgga ggcaacgtct tctgggtgca
     9361 aaaaaaatca tccatccggc tggtcagcaa ctgtagttgt taatgtgaca gagccattgc
     9421 ccatgatagt gtccattaaa aggatggaca ctatttcccc ggaacctgaa ctcaccgcac
     9481 aggcgttcta cataaaacgc ttacgcttca ttgttgactc ggagtcgact tcagataaat
     9541 cacgctttac ccttgatgga gcctgtacat agatttgtgt aattgcctga ttttgatatg
     9601 ttcaattcaa catcaaatga aggttaaatt atggacgaca aacaattgca ggctcaggct
     9661 gcgttcagca aagcatcgca accggcgata gatgcttcat taaatttaag attcagcttc
     9721 ctcttcagcc acccgtacgc caatcttcaa cacttcatta tcttctttct cggccaccgt
     9781 ccagatcatc ccggcaaact ctacctggtc accgacaacc ggtgccgtgc ctaacaactg
     9841 ctggacaatt tcacccagcg tttgctgctt atcacgatat tctcgcccgt cttccaggcc
     9901 atatatcagc gcaacatcag catatttggc gctggcttcg agaatgaagt caccaaagaa
     9961 gcgttgatcc agcgcgaccg gcggcgactg gctgaacagt ttaccgagcg ccggaagatc
    10021 gcgttcccga ccaattacac acaacacatc gccttcgcgc agtcgggtgc tgccggtagg
    10081 atgaagcaac tggttatcac gaaacagtgc cgcaatacgc gtctcttttg gcatatgcaa
    10141 atcgcgcagt gccgcgccca cgcaccattt atcggcactc aattgataaa caaactgctc
    10201 ccacggattt tccggatgaa tatccaggcc aacgcgtgac accggacgtc ccactggcgg
    10261 aaccaccact ttggcttttt tagccgccca cgagagtgat gttccctgca agagcagtga
    10321 aaccagaacc acaaagaagg cgacattaaa gaacagacgt gcattctcca gccccgccat
    10381 catcgggaac acggccagga tgattggcac cgcgccgcgt aatcctaccc agctgataaa
    10441 cacgcgctca cgcagattga aaccgcggaa ggggagcaat ccagcaaata ccgaaagggg
    10501 acgggcgaag aatatcatcc atgccgacaa aattagcgcc ggaatggcaa taggcagcag
    10561 atcgcttggg ttaaccaata gccccagcac caggaacatg gcgatttgcg ccagccaggc
    10621 gaggccgtcg aaattttgca ggatgccgta gcggttgcga atcggccgat tacccagcag
    10681 aaaaccgcac agataaacag ccagaatacc gctgccttcc agcgccgtgg ttaaagcaaa
    10741 aattaaaata ccgccgctta atgccagcaa tggatataat ccggcaggca gggcgatgcg
    10801 attaatcatt tgcagcagta aataaccacc gccaagtccg atgacaatcc cgaggccaaa
    10861 ttgctgcaga atatcgacca cgaacatcca actgacactg ctttcatgtt gctggatcat
    10921 cgcaatcagg gtaatcgtca gaaagaccgc cattggatca ttactgccgg attcaatttc
    10981 cagcgtcgag ccaacacgtt cgttaagccc cttaccaccc agcagagaaa agaccgctgc
    11041 agcatcggta gagccgacga tagcgccgat taataagcct tcaatcaaat caagattaaa
    11101 cagccacgcc gccatcatgc cggttaaacc agaggtgata agcacgccca gcgtcgccag
    11161 cgacagtgcc ggtcctaacg ccacacgaaa ggaactggcc tgagtgcgca tcccgccgtc
    11221 gagcaaaata atcgccagtg ccaggttact taccatgtag gcgaaggggt aattatcaaa
    11281 cgggataccg ccgacgccat cgactcctgc cagcatgccg atcgccagaa aaataaccag
    11341 aataggaatg ccaagacggg aagaaaatga actaagtaat atactgctgg tgacaaggat
    11401 ggatcctaag atgaaaaggc taattattgt tgtggcatcc aacggtcggt tactcctgat
    11461 tacgctgtct cttatataaa ccctaccata ttagcggcag agacagcgtt ttacttagcc
    11521 ctgaagcgtg atttttttac attttaagaa caggatgacc gctaatcgtc aactgagtgc
    11581 cttcccgggt gttattcaga atggcatgtg cgcccaacgg cagcgtaacc gtgcgttgtt
    11641 catgaccaaa atcgaggccg gtaatgagtg gaatcgacag gcgggaacgc aaaaacgcgg
    11701 acactgactc caggttgtaa cctgcgtcat aatcattggg cgtgctgccg ctaaagctac
    11761 cgagaataat cgccttctga cgtggcaaaa ttcccgcatg atagagctgc aacagcatac
    11821 gttcgacgcg gaaagggtgc tcgttaatat cttccagtac caggataccg ttctcaattt
    11881 ttggcatcca cggtgtacca atcagtgaaa tcagcatcgc aagattgcct ccccacaacg
    11941 tgccttcggt ctgacatgtc gggccttccc cttgccattc aatggtgaag gttttattgc
    12001 gtaacgccag ccagaaatgg tgctcggtaa aggcgttcag ttcatccgca ccaaagtttg
    12061 ctaccagcat ggggccgcta aaggtgatga cattgccttg cgccagaaga ccgcactgaa
    12121 tggcggtaaa atcgctatgt ccgcaaatga gtaacgggtc atgttgttgg cgagccacca
    12181 gtgcctgcca gtcaatatct gccagtaaac gactggcacc gtaaccgccg cgtacagcca
    12241 gcacgatggt gttgggtgta gtcagtctgg caagggaatt gagatcttcc agacgctccg
    12301 tttccgtacc cgcaaaacgc tcacaacgac gggcaataac ctcgacgtta ttgacttgat
    12361 gccccgcgtc ggtaaggcgc tggataccac gcagcgcggc gtgctgttta atgctgtaac
    12421 ccgatggggc aattaagtga aacagagaca tggcaattcc ttgctgacaa cagaaacgaa
    12481 atgtatatca tgccgcttag gtgtgccgtt gtcacctcaa cggcgattcc aggctataag
    12541 gatagaagaa gtgaaattga gatggtttgc ctttttgatt gtgttattag cgggttgttc
    12601 atcaaagcat gactatacga acccgccgtg gaacgcgaaa gttccggtgc aacgtgcgat
    12661 gcagtggatg ccaataagcc agaaagccgg tgcagcctgg ggcgtcgatc cacaattgat
    12721 cacggcgatt atcgctatcg aatcgggtgg taatccgaac gcggtgagta aatcgaatgc
    12781 cattggtttg atgcagttaa aagcttcaac ctccggacgt gatgtttatc gccgtatggg
    12841 ctggagtggt gagccgacga ccagcgagct gaaaaatcca gagcgtaata tttcaatggg
    12901 ggcggcttac ctgaatattc tggaaaccgg cccgctggca ggcattgaag atccgaaggt
    12961 actgcaatat gcgctggtgg tgtcatacgc taacggggca ggtgcgctgc tacggacttt
    13021 ctcgtcagat cggaaaaagg cgatcagcaa aatcaacgat ttggatgctg acgagttcct
    13081 cgatcacgta gcgcgcaatc atcctgcgcc gcaggctccg cgctatatct acaaacttga
    13141 gcaggcactg gacgcgatgt aaatcagtcg cgtactttgt ccgctttctc ccgggcttcc
    13201 cgctcgagag agaaaataat ccgttgtaat tgccgctcca ccgtcgggct gacgttaaga
    13261 aaacggaagc tcagacgggg agtggtgatg gtttcattct tgccatcaat cactttgcgc
    13321 tcgctgatgg agattaactg ggcgtcaaag tgaaaaacac cccattgccc catgttgact
    13381 tcaatctgag cgaagcgcat gccttcttgt aattcggcag gctttgctgt ttccagtaat
    13441 gcgcccatgc cgcctaacga caaatcatac aggcggaaac gtaacgtact gttatccgcc
    13501 agtttggtct ggcaaaaata aggcggatgg agtggggcgg agatgcggaa atatcggcgt
    13561 cgttgtacaa accataaggt gggaggcggt acggtaataa atgccggaag ctgcaagtat
    13621 tcactctgct gtagttgttc aacagtaaac tcgactttcg caccctgagt ttcggcggta
    13681 atggtaatat gctgcgcctt tagcacggcg atgttgtctt cggcttgact gccgaaatcc
    13741 agcaccagtt tatccggggt tattgccagt attttgctga tcagctgccc gccattccaa
    13801 ctgagacgca aaggaattgc ggctttgtgc aaatcgcgta acacgcccag gacggctaac
    13861 ggattttgtt tcaggaactg ctcatggtaa tgactcacgc ggaaaaactc ctgactgaca
    13921 aactgtcttt ggtttatcgg tcacagttaa caaaacttaa tacaaatgcg tgaatatttt
    13981 ttacatgttg ttcttaaatc agccgacata cgcccagcat cgatcccgcc cttgcctata
    14041 cttagagcgt tgacgtaagc atttcttgcg tccgattcat cgaaacgagg gcttgaacat
    14101 gggaattatt gcctggatta tttttggcct gatagccggc attatcgcca agctaatcat
    14161 gccggggcgt gatggtggtg gatttttcca gacctgtatt ctcgggatag tcggtgcggt
    14221 ggtcggcggc tggctggcga ccatgtttgg cattggcggc tccatcagcg gttttaattt
    14281 gcacagcttc ctggtggcgg tggtgggagc tattctcgtt ctgggcttat tccgcctgct
    14341 gcgaagagaa taagattttc ataaggcgga tagcgataca gatgccgcta tccgcttttc
    14401 acatcagaac gtatattcga cacctgcttt acgggtgaaa aaaatcaata tagcacttcg
    14461 atatccgata aagtcggttc ccggtaacaa caacagaatc cttttttaat tattgtttcg
    14521 ttgtttttgg tgtgattcag aaaataaata ttttagacag tagccagtca ggcgttaaaa
    14581 atggggcgga tagtgaggcg gttttggctg cgcttattga acatttagaa ttgatcaatc
    14641 cttctggcag attaagccaa aatacccgtt cggcaatgct tcagttacgc gaagaggaat
    14701 ggtctgaatt ctttttttgg ttgctaaatt ctctggagtg tctggattat gtgattatta
    14761 atttaactcc agaaagcaaa aaaacgttga tgagtgaaca ccgtaacaat attcaggtag
    14821 caatcgatgc tttatatagg caacgtcggt gtaaatcacc gggggatgat agtgaaacac
    14881 ttacccgtag aaatgacgct atcttcggca accacgtatg gcaaacattt gcgcaatact
    14941 tcccgccagg acttgaaaaa ccggctgtgt agaaaacacc atagctatgg tgttatctgc
    15001 cgacaacgcc atccggagaa gccgaatgac gttgtcggag taagcgctgg ttaaggtgtg
    15061 ggttgtgcct ctttggttga gggttgcgtc gttgctgact taacggtcgg acgcgtcgcc
    15121 ggaacattgt cacacggttg ctctttcggg cagatcaaat ccagcatttt cagcgtcacg
    15181 ccattggtcc agccaaagcc atcctgtaat ggatattcgc cacctctttt tgcccgtagt
    15241 tttgtaatcc ttctgtcgcg acccactgta acggtgccca gccatttggc gcatcccatt
    15301 gttgcccact tttcaccgac gtggtgttca ggccgccggg ttgcagcaga tgtgttttcg
    15361 tcgccgtcgc cattttgttg gcgcgatctt tcgctgccgc attgacgtac agcgggaaca
    15421 gggcggccgc ggttaactga ttgcgcactt tatgactttt caggtcgtaa tcggcatacc
    15481 agccttgttg atcgttccac aggtattttt cgattccttt ttgacgggca ttcgccagcg
    15541 tttcgtactg gtttgccatc gcgttatctc ctgcagcttt gctggcgcgg gcgaggattt
    15601 tttccatttt aaacatcagg ctgttcagat cgaccggtac gatgctggtg gtgcgtaagg
    15661 tatttaactg ctgcgggttg tccatccagc gcgagctgaa atcccagcca gacgcagcgg
    15721 cagagcgcag gtcgcggtaa atttcagtgg caggtcgatt cggattgctt ttggcggtgg
    15781 caatatcttc cacccatgac tctggtcgtg gcgtatcgcg atcgtcccag tagcggttga
    15841 gaagggtacc atcctgaagt ttgacaacgc gtttttcctg ttgtccggct tgcaggtttt
    15901 caacaccgtc catccagtaa gcatattctt tttgcatttg cggcaggtat tgcttcaacg
    15961 cggcatcgcc ttcatgctgc gccagtaact ctaccatcag ggcaaagaag ggcggttgcg
    16021 agcggcttaa atagtaactg cggttgccgt tgggaatatg accgtaagtg tctatttcat
    16081 gagcaaaatt ggccaccata tccgcgactt tatcccagtg accgctttca gcaagtccta
    16141 acatggtgaa gtaactgtcc cagtaatata cctcgcgaaa acgtccgccc ggcacgacat
    16201 aaggttccgg cagcggtaac agagaatccc atttttcggt gttttcggta gaacgcgtta
    16261 ataccggcca aagtccgtca atatgttcgc gcagtgactg cccctctggc ggaacatatt
    16321 tctcgccttc tttcggcagg gtgaaattga cgttaacgaa atggcgcaga tcaaatccgc
    16381 tctggttttg ctgcatccga taatcagcgg atcgctgttc ggcacggcat cggcaaaggt
    16441 tttttggtcc ggaaaaagtt tggcgttttg cacatcatta aacagcggcc ctaataaaat
    16501 atcaggcggc tatggtgtta ccggtgtttc ttctgcctgc accgatagcg cagcgaaaca
    16561 caaaaa
//
LOCUS       DAJTNN010000085        16554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTNN010000085 DAJTNN010000000
VERSION     DAJTNN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.085"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(28..2217)
                     /locus_tag="OO150_002410"
     CDS             complement(28..2217)
                     /locus_tag="OO150_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCR5516523.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2426..2501)
                     /locus_tag="OO150_002411"
     tRNA            complement(2426..2501)
                     /locus_tag="OO150_002411"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2466..2468),aa:Ala,seq:ggc)
     gene            complement(2541..2616)
                     /locus_tag="OO150_002412"
     tRNA            complement(2541..2616)
                     /locus_tag="OO150_002412"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2581..2583),aa:Ala,seq:ggc)
     gene            2837..3196
                     /locus_tag="OO150_002413"
     CDS             2837..3196
                     /locus_tag="OO150_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5516524.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3198..3590
                     /locus_tag="OO150_002414"
     CDS             3198..3590
                     /locus_tag="OO150_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5516525.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGVRAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="OO150_002415"
     CDS             complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="OO150_002415"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5516526.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            5317..5392
                     /locus_tag="OO150_002416"
     tRNA            5317..5392
                     /locus_tag="OO150_002416"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5350..5352,aa:Val,seq:tac)
     gene            5437..5512
                     /locus_tag="OO150_002417"
     tRNA            5437..5512
                     /locus_tag="OO150_002417"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5470..5472,aa:Val,seq:tac)
     gene            5559..5634
                     /locus_tag="OO150_002418"
     tRNA            5559..5634
                     /locus_tag="OO150_002418"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5592..5594,aa:Val,seq:tac)
     gene            5639..5714
                     /locus_tag="OO150_002419"
     tRNA            5639..5714
                     /locus_tag="OO150_002419"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5672..5674,aa:Lys,seq:ttt)
     gene            5834..6175
                     /locus_tag="OO150_002420"
     CDS             5834..6175
                     /locus_tag="OO150_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5516527.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLDVSLLNP
                     VEKTTHIDIYI"
     gene            complement(6166..7092)
                     /locus_tag="OO150_002421"
     CDS             complement(6166..7092)
                     /locus_tag="OO150_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5516528.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            7182..8180
                     /locus_tag="OO150_002422"
     CDS             7182..8180
                     /locus_tag="OO150_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5516529.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(8177..8395)
                     /locus_tag="OO150_002423"
     CDS             complement(8177..8395)
                     /locus_tag="OO150_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5516530.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(8397..10412)
                     /gene="ligA"
                     /locus_tag="OO150_002424"
     CDS             complement(8397..10412)
                     /gene="ligA"
                     /locus_tag="OO150_002424"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5516531.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIVGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(10483..11469)
                     /gene="zipA"
                     /locus_tag="OO150_002425"
     CDS             complement(10483..11469)
                     /gene="zipA"
                     /locus_tag="OO150_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5516532.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            11699..12460
                     /gene="cysZ"
                     /locus_tag="OO150_002426"
     CDS             11699..12460
                     /gene="cysZ"
                     /locus_tag="OO150_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5516533.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            12645..13616
                     /gene="cysK"
                     /locus_tag="OO150_002427"
     CDS             12645..13616
                     /gene="cysK"
                     /locus_tag="OO150_002427"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5516534.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            14000..14257
                     /gene="ptsH"
                     /locus_tag="OO150_002428"
     CDS             14000..14257
                     /gene="ptsH"
                     /locus_tag="OO150_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5516535.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            14302..16029
                     /gene="ptsI"
                     /locus_tag="OO150_002429"
     CDS             14302..16029
                     /gene="ptsI"
                     /locus_tag="OO150_002429"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5516536.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            16070..>16554
                     /gene="crr"
                     /locus_tag="OO150_002430"
     CDS             16070..>16554
                     /gene="crr"
                     /locus_tag="OO150_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5516537.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLPGSVTVG
                     ET"
BASE COUNT         4181 a         4258 c         4061 g         4054 t
ORIGIN      
        1 gataattccc cgtttttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa
       61 atacccttgc agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc
      121 gacaaactcc gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac
      181 aatcatcgca tccagcgtgt tcgtgacaat atctttcaca aagacgccat caattttgat
      241 gatatcagcc tgcaaacgct ttaaccgttc gtagttggca tagccggtgc caaagtcatc
      301 aatcgcaatc cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc
      361 gttagaaaac gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata
      421 acgcttaaac agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat
      481 taaattgact gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa
      541 caaggattcc agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg
      601 cagaaactta tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc
      661 ataaccttca ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgatc
      721 taacgccgtg cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat
      781 ctcctctctg ctatccagcg ccagcacatg atgatgtgcg caggattgct ccgccagcca
      841 gcttaactgc cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc
      901 agcaccatag cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat
      961 gttaaccata tgctggagtc gcccttccgt ttccggcccg ctcagcacta acagcagttc
     1021 actacccggc aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggca
     1081 aattgagcgg atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag
     1141 attatcaatg cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttccaa
     1201 cgcacggaag ttgggcagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg
     1261 attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga
     1321 aatcagcacc gcaagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt
     1381 ctggttgtaa ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata
     1441 gcgaagctta ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc
     1501 aataaagtcg ttttcataag gtgtgcacaa cagaagtaga agcacgctta acgctgccag
     1561 ccagcttaag gtaaatgcgc gtttctcttt gcccaacgac ggcgcgatat ccctgcgcca
     1621 caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga aaagcatgtt
     1681 gtaaatcagc acagcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc
     1741 atcgccgaaa aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat
     1801 gctgcatttg atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata
     1861 tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc
     1921 acacaacacg acaaacgtct ggctaccgag catgaccgca gtctgtaaaa aagagagatg
     1981 aaagttccac agattggtgc aaaacatccc gcataaaacg ggaacgacac ctcgccagcc
     2041 aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact
     2101 ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag
     2161 cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag
     2221 tacactactc tcactgatcg gagttttcag gcacacaaat aataagttta ttcgcatcgc
     2281 gaagcaaaca gggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg
     2341 gcactaaccc ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc
     2401 cgctttgcag cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct
     2461 tgcatgccat gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca
     2521 aatttgctgg gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
     2581 gccatgcaag cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga
     2641 cgggcggcat catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt
     2701 caatcgctga aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta
     2761 ttcatgtcac ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata
     2821 cattcaggga attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac
     2881 cggctatagc cgccagacca ttaataaatg ggtacgcaag gaaggctgga caacgtcacc
     2941 aaaacctggc gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga
     3001 atatattcgc aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga
     3061 tgcgccgctt gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa
     3121 acagtttaca tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat
     3181 tcgcgatagc aaataatatg aaaagattac gcaataaaat gaccaccgaa gaactggctg
     3241 aatgcctcgg tgtggctaaa caaactgtta accgttggat cagagaaaaa ggctggaaaa
     3301 cggaaaaatt tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag
     3361 tttgcgagtt tattcagaac acgccagcct tccataacac accaatgttg atggaagctg
     3421 aagagagaat tgctgaatac gctcctggag tacgcgcccc ggcgtatcga caaatcatca
     3481 acgcgataga taatatgaca gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg
     3541 aaggaattcg caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg
     3601 gcaggatatt atctcctgcc gtttatcttt tatgcgctaa ttactgctgg ttttcgcgtt
     3661 cagcaataaa agccagcgct ttgttgatac gctcaatact gcgggtccta ccaattgcgt
     3721 gaacggtaac atccagtgcc ggagactgcc ccgcaccggt tacggcgaca cgcagcggca
     3781 taccaacttt acccataccc acttccagct catccgccgt cgcctgaata gcgtgatgaa
     3841 cgttttcagc ggtccagtca gtaatcgcgg ccagtttgtc acgaaccact tccagcggct
     3901 gacgcgctac cggacgcaga tgttttttcg cggcgtcggc atcgaactca gcaaaatctt
     3961 cgtagaaata acggcagctc tgtgccatct ctttcagcgt cttgcagcgt tcgcccagca
     4021 gtttcaccag atcagccagc tgcgggccgt tacgggtatc gatattttcc tgctcaatgt
     4081 gccactgtaa gtgagtagca acatactccg gcggcagcgc gttaatgtag tgatggttca
     4141 gccacagcag cttgtcggtg ttgaacgcac tggcagattt gctgacggca ttcagggtga
     4201 agtatttgat catctcttca cgagtgaaga tttcctgatc gccgtgggac cagcccagac
     4261 gcaccagata gttcagcagt gcttctggca aataaccgtc atcacgatac tgcattacgc
     4321 tgaccgcccc gtgacgtttg gacagttttt taccgtcatc gccgttgatc atagaaacgt
     4381 gcgcgtaaac cggcaccggc gctttcaggg ccttaagaat gttgatctgg cgtggcgtgt
     4441 tgttgatatg gtcttcgcca cggataacgt gggtgatttc catatcccag tcatcgacaa
     4501 ccacacagaa gttataggtt ggggaaccat cggtacggcg aataataaga tcgtccagtt
     4561 cctggttgct gaactcgatc ggaccacgga tctgatcgtc aaaaacaaca gaaccttcct
     4621 gcgggttagc aaaacgtaca acgcacggtt catcatcagc gtgatgctca tggctgtggc
     4681 ggcagcgacc gtcataacgc ggcttctcac ctttcgccat ttgctcttcg cgcagcgctt
     4741 ccaggcgctc tttagagcaa tagcatttat aagcagtgcc ctcttccagc atctgatcga
     4801 tcaccgcgtt gtagcgatca aaacgtttgg tctggtagta cggaccttca tcccactcca
     4861 ggctcagcca gttcatgcca tccataatgg cttcgatagc ttccggcgtg gaacgctcaa
     4921 gatcggtgtc ttcaatacgc agcacgaact caccgccgtg gttacgtgca aaaagccagg
     4981 agtaaagggc agtacgcgcg ccgccaacgt gcagatagcc tgttgggctt ggcgcgaagc
     5041 gagttttgat tttcatgaaa tggccttacg tttagaaaga tgccgacaac cggcaaatcc
     5101 tggaaaaatt gagtgggcga tattctatca ctcccgcctg attcctcaat gtagttcggg
     5161 tttctacctt acgcattatt gttttcgttg acaaattgcg caacacggtc tattttgcga
     5221 tcatcttgct taaatttacg acgaacgaac aatttcttta gaaaatgcgt tgactcattt
     5281 tgaactctcc ctataatgcg actccacaca gcgggggggt gattagctca gctgggagag
     5341 cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac caactacttt
     5401 atgtagtctc cgccgtgtag caagaaattg agaagtgggt gattagctca gctgggagag
     5461 cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac cactttctca
     5521 ccagctaaat ttcttgtaac aatgtgaagt accgaagtgg gtgattagct cagctgggag
     5581 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accacttcgg
     5641 gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc
     5701 tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct tttttgctat ctgcgatttg
     5761 taagattgat ttttcttctc aagttccttg ttcttctacc gatattacct cgtacagtga
     5821 cgaggactga acaatggcca ctatctcttc aacttcaatt ccttccatac aaacacagag
     5881 tagtaacagg gcatcacaag gtagcgatgt tgcttcacaa atcgcgcgga taagtcagca
     5941 aatcatcaaa ctcactcaac aaattaaaga gattgtagac actagcggca gtgctgaaga
     6001 caagcaaaaa caggccgaat tgattcaaca gcaaatcacc ctactggaaa cacaactggc
     6061 gcagttgcag aaacagcagg cagaaaaggc gcaggagaaa gagcagcgtt tgtctctgga
     6121 tgtatccctt ctaaatcctg ttgaaaaaac gactcatatc gatatctata tctgatacaa
     6181 cggatcccct tcccgaccag ccattagcgc attgccctgg tctcgtacca cgtcccataa
     6241 cgcttcagcg gcggtggaaa gtgaacggtt cttcctgcgc accagcatca actggcgttc
     6301 aacaaccggc gtaatgcgct ttaccaccag tgggctacct tcaggcaatg gtagcgccag
     6361 tgcgggaaga atactgatgc cgatgcctgc ggctaccatc gggaacagcg tcgccggatg
     6421 tccaatctcc tgcacaatat tcgcctgaat accattgcgc gccagcgctg cgtcaatcag
     6481 cggtcggctg cctgacgcgt aatcctgcaa caccaatttc gctccctgta atgcctgcca
     6541 gggtacataa tcttccacgg ccaaagcgct atcgcgatgg caaagcagaa agaaaggctc
     6601 ggaaagaatc gcttcacatt gcagatcgcc cactgggcct ggatcgatga cgatgccaaa
     6661 gtcgacatct ccctggcgaa tactttccat cacccactgc tgcggacgat cgtgcaggac
     6721 aaactgaata tctggatagc ggcgatggct ttcagcaatg cattgcggaa taagatgcgc
     6781 ggaaatggtc tggctggcag cgacccgaac ttttccgctc agttgttgcc ccatacgccc
     6841 ggtatcgcgc aacgtgctgt taagttcatc cagcagtcgc tcaagacgca aagccaactg
     6901 ctgccctgca tctgtaagca ccacttcacg cgtggttctg tccagcaggc gaacaccagt
     6961 atgattttcc agctccttca cactgtgact tactgccgac tggctcaagc cgatacgctc
     7021 tcctgcacga ctaaaacttt tctcctgcgc tactgtgacg aaaaccttta attgttttaa
     7081 agaataattc atctgttttc ttcatgaatg gatgcaataa atcaatttta tttctcaaac
     7141 tggaagaagc acaatagaac catcgatcat ctggagtctt tatgaaactt tttcgtatcc
     7201 tcgatccttt caccttaacc ctgatcacgg tggtgttgct ggcctctttc tttccggcca
     7261 gaggcgattt cgtccccttc tttgaaaatc tgaccaccgc agctattgcc ctgctgttct
     7321 ttatgcacgg cgcgaagttg tcgcgtgagg cgattattgc tggcggtggt cactggcgac
     7381 tgcatttgtg ggtaatgtgc agcaccttcg tgctgtttcc gattctgggt gtactgtttg
     7441 cctggtggaa accggtaaat gtcgacccga tgctctactc cggttttctc tacttgtgca
     7501 ctctcccggc taccgtgcag tctgcaatcg ccttcacgtc aatggcgggc ggtaacgtcg
     7561 cggcggcggt ttgttctgcg tcagcatcca gcctgctggg aattttcctt tcaccattgc
     7621 tggttggtct ggtgatgaat gttcacggtg cagggggcag ccttgagcag gtcggtaaaa
     7681 ttatgctgca actgctgctg ccgtttgtgt tggggcatct ttcccggccg tggattggtg
     7741 actgggtgtc gcgcaataaa aaatggattg cgaaaactga ccagacgtcc attctgttgg
     7801 tggtttatac agcgttcagc gaagccgtcg ttaatggtat ctggcataaa gttggctggg
     7861 gatcattgct gtttatcgtg gtggtcagct gcgttcttct ggctatcgtg attgtagtta
     7921 acgtctttat ggcacgccga ctgggcttca ataaggcaga tgaaattact atcgtctttt
     7981 gtggttcgaa aaagagtctg gcaaatggca tcccgatggc aaacattctg ttccccacat
     8041 cggtgatcgg tatgatggtg ctgcccctga tgattttcca tcagatccaa ttgatggtct
     8101 gtgcggtgct ggcgcgtcga tacaaacgcc agaccgaaca gttacaggcg cagcaggaaa
     8161 gcagcgccga taaagcttaa agcggacgct tcaggggctg gaccagttgc gtcagcccct
     8221 cggttttaat cagcagcgtg atttgcatta gctcaccgag cttccctgcc gggaactcat
     8281 ctttgcgggc aaaccacagc agatactcct ccggcaggtc gattaagcga cgccctttgt
     8341 atttgccaaa cggcattatc gtattggcta tttcaatcag ctgctctttt tccatctcag
     8401 ctacccagca aacgcagcat ttccgcttcg tcgatgactt caatgcccag ttcctgcgcc
     8461 ttcgccagtt tagatcctgc agcttcaccc acaatcacca gatcggtttt cttcgacacg
     8521 ctgcccgcga ctttcgcgcc cagttcgacc agtcgagctt tagcgtcatc acgcgacatc
     8581 tggcttaagc tgcccgtaag caccacggtt ttaccagcaa acgggctgtc aatctcttcc
     8641 gcgttgataa cgatcggcgc aggccagtga acaccttccg ccaacagctc gctgatgaca
     8701 ttgcggttgc tttcttcggc aaagaagttg tgaacgtggg atgcaacgac aatgccaaca
     8761 tcaggcacct tttgcagctc ttcaatcgaa gcggcttcca gcgcttccag cgtgccgaaa
     8821 tatgccgcca gacctgctgc ggtggcctcg ccgacttcac ggatgccaag tgcatagagg
     8881 aagcgagcaa aggtggtttc tttcgctttt tccagcgcgt taaccacgtt ttgtgccgat
     8941 tttggcccca tacgctccag tccggtcagt ttgcctgcgg tgagtttgaa cagatctgcc
     9001 ggagtgtgga catattcttt ttcaaccagc tgatcgatga ttttgtcgcc cattccgtca
     9061 acatccatcg cacggcggga aacaaagtgt ttcagcgact ctttacgctg cgcaccgcaa
     9121 atcaggccac cggtacagcg ggcaaccgct tcaccttcca cacgctcaac gtcagaacca
     9181 cataccggac aatgcgtcgg gaatacaacc tcacgggtat cttccgggcg ttcagaaagc
     9241 acgacgttaa ccacctgcgg gatcacgtcg ccagcgcggc gaatcaccac tttatcgcca
     9301 atgcgtaaac caagacgttc gatttcatcc gcattgtgta aggttgcgtt actcaccagc
     9361 acgccagcaa catggacagg ttccagacgc gcaacaggcg taatcgcgcc agtacgccca
     9421 acctgaaact cgacgtcacg cacaaaggtc atctgctcct gcgccgggaa tttaaacgct
     9481 accgcccagc gcggggcacg cgcgacaaag ccaagctgct cctgctgttc cagtgagttg
     9541 accttaatca ccacgccgtc gatatcaaag cccagcgtcg ggcggtcttc ttccactttg
     9601 tgatagaacg ccagcacttc ttccgccgat tcacaaagcg ttacccgatc gctgaccggc
     9661 aacccccact ttttaaattg cagtaaacgg ccaagatgag tatccggcag ctcgccacct
     9721 tccagaacac caacgccata gcagaaaaaa gtgagcggtc gcttcgctgt aatacgcgga
     9781 tcaagctgac gcagtgaacc agctgccgca ttacgtgggt tagcaaaaac tttcccgccc
     9841 gtgcgtcgcg catcttcgtt aatcttttcg aaccccgcct gcggcaggaa cacttcacca
     9901 cgcacttcca gacgcgccgg gatattctct ccatgcagct tcagcggaat ggcgcgaata
     9961 gtacgcacat tagacgtgat atcttccccg gtggtgccat cgccacgggt cgcggcactg
    10021 actaaaacgc cattttcata cagaatactg acggcaaggc catccagctt cagctcacag
    10081 caccaggtga ctttctcgtt gtttttcaga cggtcctgca cacgtttgtt gaaagcaaga
    10141 aagctttctt catcaaaaac gttatccagt gacagcattg gtacttcatg gcgtatctgg
    10201 ctgaaagccg ccagcggcgc agcacctaca cgttgggtag gcgaatcagg cgtaatcagt
    10261 tctggatgtt tagtttccag ctcgcgcagt tcgcgcatca gcctgtcgta ttcagcgtcg
    10321 ggaatttccg gcgcatccat cacatgataa agatattcat gatggcgaag cgtcgttcgc
    10381 agttctgtca gttgttgttc gattgattcc atatcgcacc atcaatgcta aaaacccccg
    10441 acaagcgggg gttcgaagaa gagttaattt gccttaagtg tatcaggcgt tggcgtcttt
    10501 gacttcgcgg atgatgtcct ggtactcgcg caatttctgc ggagtcatca tacggcgctg
    10561 atcgtcaagc acgacaccgc ccacttcatc ggcaatatgc tgcgcagatt gcagcatcag
    10621 cttgaagttc tgcagctcgt caccgtaaga cggtacctgc ataaagatag tgacacccgg
    10681 agtagtgaaa tccttcattt caggatcaaa ggttcccggt ttcaccatat tcgccaggct
    10741 gaataacgcc gggccgctgc catccgggct aagatgacga tggtaaatat tcatatcgcc
    10801 aaaaatgaag cccgcttgtt gaatgctgtt aagaagcagt tcaccgttta gctcgctacc
    10861 gtgatgcgcc gcgacgttca tgataatcac cgcttctttg cgcttcggtt tatccataac
    10921 tggagccggt tccgctacag gctcaggctg tggtgccgct acgggttctg caggctggaa
    10981 agcctgttgt gccggttgcg gtgctgaatg cacaggctgc ggcgctggcg cgacctgtgg
    11041 cgaaactggc tgctgcaacg gctgttcagg ctgcggctga tagacaggct gctgcaccgg
    11101 ctgcgctgga cgcggagcat gttgcggcgg tacctgcgct tcaggcggct gctggaccgg
    11161 ttgacgcggc tgcgcagacg cataaggcgg ttggtactgg tgttgcggcg acggacgagc
    11221 agcctcatgc tcctgagcgt tagccggggc atgattcacg cggtgaacac gaacctcacc
    11281 aacgccctca tcatcttcga catcctcgtc ataagaatcg tcgtcacgtt ttgacttcat
    11341 tcgttttaat ggccgatcgc ggaacataga agatcgttct ttacggctgg tccagaaacc
    11401 atgtaccagt aaagcgatta tggcgatcgc gccaacaatg attaatatca gacgcaaatc
    11461 ctgcatcatt atattctctg ttgttctaac accttgccac cacggcaaac atttactcac
    11521 taagagtatt tgccgattac ctcaagtgca agtgcactat taactttcac agcacaaaga
    11581 tagatgaaat cgtgcttttt gctgtttttt cgaacatatc ctaactgtcc attgcgcaat
    11641 tacccggtaa aatacgcaga attttcctag gattggtcaa aaggagctca tcctgattat
    11701 ggtttcatca ttcacatctg ccccacgcag cggtttttac tattttgcgc aaggctggaa
    11761 gctcgtctcg caacctggga ttcggcgttt cgttatttta ccgctgctgg tcaatatttt
    11821 gttgatgggg ggcgcattct ggtggctctt tacacagctc gatgtctgga tcccgactct
    11881 catgagttac gttccggact ggctacaatg gctgagttat ctgttgtggc ctctggctgt
    11941 catctctgtg ctgttagtgt ttggctattt cttctccacg attgctaact ggattgccgc
    12001 tccgtttaac ggtttattgg ctgaacaact ggaagcacga ttgactggcg ctacaccgcc
    12061 agataccggg attttcggta tcatgaaaga tgtgccgcga atcatgaaac gcgaatggca
    12121 aaaatttgcc tggtatctgc cgcgcgcaat tgtattacta attctttact tcatccctgg
    12181 tattgggcaa accgtcgcgc cggtactgtg gttcctgttt agcgcctgga tgttagccat
    12241 ccagtattgc gattacccct tcgataacca caaagtgccg tttaaagaga tgcgcaccgc
    12301 cctgcgcaca cgcaaaatca ccaatatgca gtttggtgct ttaaccagcc tgtttacgat
    12361 gatcccgctg cttaatctgt tcatcatgcc cgttgccgtt tgtggcgcga cggcgatgtg
    12421 ggtcgattgc tatcgcgata aacacgcgat gtggcggtaa caatctaccg gttattttgt
    12481 aaaccgtttg tgtgaaacag gggtggctta tgccgcccct tattccatct tgcatgtcat
    12541 tatttccctt ctgtatatag atatgctaaa tccttacttc cgcatattct ctgagcgggt
    12601 atgctacctg ttgtatccca atttcataca gttaaggaca ggccatgagt aagatttttg
    12661 aagataactc gctgactatc ggtcacacgc cgctggttcg cctgaatcgc atcggtaacg
    12721 gacgcattct ggcgaaggtg gaatctcgta accccagctt cagcgttaag tgccgtatcg
    12781 gtgccaacat gatttgggat gccgaaaagc gcggcgtgct gaaaccaggc gttgaactgg
    12841 ttgaaccgac cagcggtaat accgggattg cactggccta tgtagctgcc gctcgcggtt
    12901 acaaactcac cctgaccatg ccagaaacca tgagtattga acgccgcaag ctgctgaaag
    12961 cgttaggtgc aaacctggtg ctgacggaag gtgctaaagg catgaaaggc gcaatccaaa
    13021 aagcagaaga aattgtcgcc agtaatccag agaaatacct gctgctgcaa caattcagca
    13081 atccggcaaa ccctgaaatt cacgaaaaaa ccaccggtcc ggagatctgg gaagataccg
    13141 acggtcaggt tgatgtattt attgctggcg ttgggactgg cggtacgctg actggcgtca
    13201 gccgctacat taaaggcacc aaaggcaaga ccgatcttat ctctgtcgcc gttgagccaa
    13261 ccgattctcc agttatcgcc caggcgctgg caggtgaaga gattaaacct ggcccgcata
    13321 aaattcaggg tattggcgct ggttttatcc cggctaacct cgatctcaag ctggtcgata
    13381 aagtcattgg catcaccaat gaagaagcca tttctaccgc ccgtcgtcta atggaagaag
    13441 aaggtattct tgcaggtatc tcttctggag cagctgttgc cgcggcgttg aaactacaag
    13501 aagatgaaag ctttaccaac aagaatattg tggttattct accatcatcg ggtgagcgtt
    13561 atttaagcac cgcattgttt gccgatctct tcactgagaa agaattgcaa cagtaatgcc
    13621 agcttgttaa aaatgcgtaa aaaagcacct ttttaggtgc ttttttgtgg cctgcttcaa
    13681 actttcgccc ctcctggcat tgattcagcc tgtcggaact ggtatttaac cagactaatt
    13741 attttgatgc gcgaaattaa tcgttacagg aaaagccaaa gctgaatcga ttttatgatt
    13801 tggttcaatt cttcctttag cggcataatg tttaatgacg tacgaaacgt cagcggtcaa
    13861 cacccgccag caatggactg tattgcgctc ttcgtgcgtc gcgtctatta aaaactggcg
    13921 ctaacaatac aggctaaagt cgaaccgcca ggctagactt tagttccaca acactaaacc
    13981 tataagttgg ggaaatacaa tgttccagca agaagttacc attacagctc cgaacggtct
    14041 gcacacccgc cctgctgccc agtttgtaaa agaagctaag ggcttcactt ctgaaattac
    14101 tgtgacttcc aacggcaaaa gcgccagcgc gaaaagcctg tttaaactgc agactctggg
    14161 cctgactcaa ggtaccgttg tgactatctc cgcagaaggc gaagacgagc aaaaagcggt
    14221 tgaacatctg gttaaactga tggcggaact cgagtaattt cccgggttct tttaaaaatc
    14281 agtcacaagt aaggtagggt tatgatttca ggcattttag catccccggg tatcgctttc
    14341 ggtaaagctc tgcttctgaa agaagacgaa attgtcattg accggaaaaa aatttctgcc
    14401 gaccaggttg atcaggaagt tgaacgtttt ctgagcggtc gtgccaaggc atcagcccag
    14461 ctggaaacga tcaaaacgaa agctggtgaa acgttcggtg aagaaaaaga agccatcttt
    14521 gaagggcata ttatgctgct cgaagatgag gagctggagc aggaaatcat agccctgatt
    14581 aaagataaac acatgacagc tgacgcagct gctcatgaag ttatcgaagg tcaggcttct
    14641 gccctggaag agctggatga tgaatacctg aaagaacgtg cggctgacgt acgtgatatc
    14701 ggtaagcgcc tgctgcgcaa catcctgggc ctgaagatta tcgacctgag cgccattcag
    14761 gatgaagtca ttctggttgc cgctgacctg acgccgtccg aaaccgcaca gctgaacctg
    14821 aagaaggtgc tgggtttcat caccgacgcg ggtggccgta cttcccacac ctctatcatg
    14881 gcgcgttctc tggaactgcc tgctatcgtg ggtaccggta gcgtcacctc tcaggtgaaa
    14941 aatgacgact atctgattct ggatgccgta aataatcagg tttacgtcaa tccaaccaac
    15001 gaagttattg ataaaatgcg tgctgttcag gagcaagtgg cttctgaaaa agcagagctt
    15061 gctaaactga aagatctgcc agctattacg ctggacggtc accaggtaga agtatgcgct
    15121 aacattggta cggttcgtga cgttgaaggt gcagagcgta acggcgctga aggcgttggt
    15181 ctgtatcgta ctgagttcct attcatggac cgcgacgcgc tgcccactga agaagaacag
    15241 tttgctgctt acaaagcagt ggctgaagcg tgtggctcgc aggcggttat cgttcgtacc
    15301 atggacatcg gcggcgacaa agagctgcca tacatgaact tcccgaaaga agagaacccg
    15361 ttcctcggct ggcgcgctat ccgtatcgcg atggatcgta aagagatcct gcgcgatcaa
    15421 ctccgcgcta tcctgcgtgc ctcggctttc ggtaaattgc gcattatgtt cccgatgatc
    15481 atctctgttg aagaagtgcg tgcactgcgc aaagagatcg aaatctacaa acaggaactg
    15541 cgcgacgaag gtaaagcgtt tgacgagtca attgaaatcg gcgtaatggt ggaaacaccg
    15601 gctgccgcaa caattgcacg tcatttagcc aaagaagttg atttctttag tatcggcacc
    15661 aatgatttaa cgcagtacac tctggcagtt gaccgtggta atgatatgat ttcacacctt
    15721 taccagccaa tgtcaccgtc cgtgctgaac ttgatcaagc aagttattga tgcttctcat
    15781 gctgaaggca aatggactgg catgtgtggt gagcttgctg gcgatgaacg tgctacactt
    15841 ctgttgctgg ggatgggtct ggacgaattc tctatgagcg ccatttctat cccgcgcatt
    15901 aagaagatta tccgtaacac gaacttcgaa gatgcgaagg tgttagcaga gcaggctctt
    15961 gctcaaccga caacggacga gttaatgacg ctggttaaca agttcattga agaaaaaaca
    16021 atctgctaat ccacgagatg cggcccaatt tactgcttag gagaagatca tgggtttgtt
    16081 cgataaactg aaatctctgg tttccgacga caagaaggat accggaacta ttgagatcat
    16141 tgctccgctc tctggcgaga tcgtcaatat cgaagacgtg ccggatgtcg tttttgcgga
    16201 aaaaatcgtt ggtgatggta ttgctatcaa accaacgggt aacaaaatgg tcgcgcctgt
    16261 agacggtacc attggtaaaa tctttgaaac caatcacgcg ttctctatcg aatctgatag
    16321 cggcgttgaa ctgttcgtcc acttcggtat cgacaccgtt gaactgaaag gcgaaggctt
    16381 caagcgtatt gcagaagaag gtcagcgcgt gaaagttggc gatactgtca ttgaatttga
    16441 tctgccgctg ctggaagaga aagccaagtc taccctgact ccggttgtta tctccaacat
    16501 ggacgaaatc aaagaactga tcaaactgcc cggtagcgta accgtgggtg aaac
//
LOCUS       DAJTNN010000086        16521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTNN010000086 DAJTNN010000000
VERSION     DAJTNN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.086"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            93..2321
                     /gene="crfC"
                     /locus_tag="OO150_002431"
     CDS             93..2321
                     /gene="crfC"
                     /locus_tag="OO150_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5516538.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAAQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2318..3201
                     /locus_tag="OO150_002432"
                     /pseudo
     CDS             2318..3201
                     /locus_tag="OO150_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            3465..4967
                     /gene="proP"
                     /locus_tag="OO150_002433"
     CDS             3465..4967
                     /gene="proP"
                     /locus_tag="OO150_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5516539.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5033..5170
                     /gene="pmrR"
                     /locus_tag="OO150_002434"
     CDS             5033..5170
                     /gene="pmrR"
                     /locus_tag="OO150_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5516540.1"
                     /translation="MSDAHTLLQVNGGECNEKPCLYESLTTVFSVLVVSSFLYIWFAT
                     F"
     gene            complement(5146..6246)
                     /gene="pmrB"
                     /locus_tag="OO150_002435"
     CDS             complement(5146..6246)
                     /gene="pmrB"
                     /locus_tag="OO150_002435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5516541.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6247..6915)
                     /gene="basR"
                     /locus_tag="OO150_002436"
     CDS             complement(6247..6915)
                     /gene="basR"
                     /locus_tag="OO150_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5516542.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6912..8555)
                     /gene="eptA"
                     /locus_tag="OO150_002437"
     CDS             complement(6912..8555)
                     /gene="eptA"
                     /locus_tag="OO150_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5516543.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTV
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8659..9996)
                     /gene="adiC"
                     /locus_tag="OO150_002438"
     CDS             complement(8659..9996)
                     /gene="adiC"
                     /locus_tag="OO150_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5516544.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTPGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10133..10894)
                     /gene="adiY"
                     /locus_tag="OO150_002439"
     CDS             complement(10133..10894)
                     /gene="adiY"
                     /locus_tag="OO150_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5516545.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11219..13486)
                     /gene="adiA"
                     /locus_tag="OO150_002440"
     CDS             complement(11219..13486)
                     /gene="adiA"
                     /locus_tag="OO150_002440"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5516546.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPSARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHAMCVKA"
     gene            complement(13685..14593)
                     /gene="melR"
                     /locus_tag="OO150_002441"
     CDS             complement(13685..14593)
                     /gene="melR"
                     /locus_tag="OO150_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5516547.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMMIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14876..16231
                     /gene="melA"
                     /locus_tag="OO150_002442"
     CDS             14876..16231
                     /gene="melA"
                     /locus_tag="OO150_002442"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5516548.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPHIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16334..>16521
                     /locus_tag="OO150_002443"
     CDS             16334..>16521
                     /locus_tag="OO150_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5516549.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMG"
BASE COUNT         3986 a         3998 c         4350 g         4187 t
ORIGIN      
        1 tttcttgcag taattataaa agccgaaaac agaactcaaa aacaatctgg ctaaataaaa
       61 gtaacaaaat ttgctttaag gaagaatttt ctatgtacac acagaccctg tatgagttaa
      121 gtcaggaggc tgaacgcctg ttacagcttt ctcgccaaca gttgcagtta ctggaaaaaa
      181 tgcctctctc tgtacccgga gacgatgcgg cacaactggc tttaccctgg agtcagccta
      241 atatcgccga acgttacgcg atgctgaata atgagttgcg taaaatttcc cgactggaaa
      301 tggtgctggc gattgtcggt actatgaaag cagggaaatc aaccaccatt aatgccattg
      361 ttggtacgga ggttctgcct aatcgtaatc gcccaatgac tgcgctgccg acgcttattc
      421 gccatacgcc cgggcaaaag gaaccggtac tgcatttttc acatgtcgcg ccaatcgatt
      481 gtttaattca acaattacaa cagcgcctgc gtgattgcga tattaagcat ctgaccgatg
      541 tgctggaaat agataaagat atgcgtgcgc ttatgcagcg gatcgaaaat ggcgtcgctt
      601 tcgaaaaata ttatctgggt gcccagccta tttttcattg tctgaaaagt ttgaatgatt
      661 tagtgcgact ggcgaaggcg ctggacgtcg attttccttt ttctgcttac gccgccattg
      721 agcatattcc cgtgattgaa gtggagtttg tccatctggc ggggctggaa agttatcccg
      781 gtcagttgac gttactggat acccccgggc caaatgaagc cgggcaaccg catctgcaaa
      841 aaatgcttaa ccagcagctg gcacgcgcct cggcggtact ggcggtgctg gattatacgc
      901 aactgaaatc gatctccgat gaagaggtcc gtgaggcgat tttggcggtg gggcaatcgg
      961 tgccgctgta tgtgctggtc aataagttcg atcaacagga tcgtaacagt gacgacgccg
     1021 accaggtgcg ggcactgatt tccgggacgc tgatgaaagg ctgtattacg ccacagcaga
     1081 tatttccggt gtcgtcgatg tggggctacc tggcgaatcg ggcgcgctat gagttagcca
     1141 acaacggtaa gttaccagcg ccagagcaac aacgctgggt ggaagatttt gcccatgccg
     1201 cgctcggcag gcgctggcgt catgccgatc tggcggacct cgaacatatt cgtcatgctg
     1261 ccgatcagtt gtgggaagat tcgctgttcg cccagccaat tcaggcgttg cttcatgccg
     1321 cttacgctaa cgcctcattg tatgctctgc gatctgctgc gcataaactg ttgaattacg
     1381 cgcagcaggc gcgggaatac ctggattttc gtgcgcacgg gttaaacgtc gcttgtgaac
     1441 aattgcggca aaatatccat caggtcgaag aaagtttgca gctattgcaa ctcaatcagg
     1501 cgcaggtgag cggcgagatt aaacatgaaa tcgagctggc cctgacctcc gccaaccact
     1561 ttctgcgtca acagcaagat gcgctgaatg cgcagttagc cgccttgttt caggatgatt
     1621 cggagccgtt aagcgagatt cgtacctgct gtgagacact gttacagacg gcgcagaaca
     1681 ccatcagtcg cgactttacg ctgcgttttg ccgagcttga atccaccctt tgccgggtgt
     1741 taaccgatgt tattcggccc attgagcaac aagtcaaaat ggaattgagc gagtcagggt
     1801 ttcgtcctgg gtttcatttt cctgtttttc acggagtagt tccccacgtc aacactcgcc
     1861 agctgttcag tgaagtcatt tcgcgccagg aggcaacgga cgagcagagc acgcgtttag
     1921 gcgttgtgcg tgagactttt tcgcgctggt tgaatcagcc cgactgggga cggggaaatg
     1981 agaaatcccc gacagaaacg gttgattaca gtgtgttgca acgagcatta agcgcagaag
     2041 tcgatcttta ttgccaacaa atggctaaag ttctggcaga gcaggtcgat gaatctgtta
     2101 cggcaggcat gaatactttt ttcgctgagt tcgcttcatg tttgacggaa ttacagacgc
     2161 gtttacgcga aagcctggct ctgcgtcaac aaaatgaatc ggtggtcagg ctgatgcagc
     2221 agcaattgca gcagactgtg atgactcacg gctggattta caccgacgcc cagctgttac
     2281 gcgatgatat tcaaacactt ttcacggcag aacgatattg accaagacgt tactggacgg
     2341 ccccggtcgc gtgctggagt cggtttatcc ccgcttttta gtggatctgg cgcagggcga
     2401 tgatgcccgc cttccacaag cccatcagca gcagtttcgt gaacgactga tgcaggaact
     2461 tctttcgcgt gtgcagcttc agacatggac gaacggcggc atgttaaatg cgccgcttag
     2521 cctgcgactg acaatggtgg aaaaactggc gtcgatgctg gatcccggtc atctggcgct
     2581 gacgcagatc gcgcagcatc tggcgctgct gcaaaaaatg gatcaccgcc agcactctgc
     2641 tttcccggag ctcccccagc aaattgccgc cttgtatgag tggttttcag cccgttgtcg
     2701 ctggaaggaa aaggcgttaa cgcaacgagg ccaggcctac tggtgcaggc aggtgatcag
     2761 agcgagcaaa tttttacccg ctggcgtgct ggggcgtata acgcctggtc gttgcctggg
     2821 cgctgtttta tcgttctcga agagttgcgt tggggggcat ttggcgatgc ctgtcgtctg
     2881 ggaagcccgc aagcggtggc gttgctgctg ggtgatttgc gcgtgaaagc gacacaacat
     2941 ctggcagaga gtatcaatgc ggccccgacc acgcgtcact attaccatca gtggtttgcc
     3001 tcttcgaccg ttctgacggg cggggatcat gctgattttt taagttggct gggaaagtgg
     3061 accacggcag ataaacaacc cgtttgctgg tcagtgaccc aacgttggca aactgtcgcg
     3121 ctggggatgc cacgactctg ttcagcgcag cgtttggtgg gggcaatggt cgaggaaatc
     3181 ttctctgtaa atttggcgta aataatcagt tacatcaatg agtcctaaac gaaatccatg
     3241 tgtgaagttg atcacaaatt taaatactgg tagggtaaaa aggtcattaa ctgcccaatt
     3301 caggcgtcaa ctggtttgat tgtacattcc ttaaccggag ggtgtaagca aacccgctac
     3361 gcttgttaca gagattgcat cctgcaattc ccgctcccct tttgcggccg tcgcgctgat
     3421 ttttctggcg tttgcggaaa tgggccaact ctgcgaggaa agctatgctg aaaaggaaaa
     3481 aagtaaaacc gattaccctt cgtgatgtca ccattattaa tgacagtaaa ctgcgtaaag
     3541 ccattaccgc agcatcactg ggtaatgcaa tggaatggtt cgattttggt gtttatggtt
     3601 ttgttgctta cgcattaggt aaagtttttt tcccgggggc tgaccccagc gtgcagatgg
     3661 ttgctgcact tgccactttc tccgttccct ttctgattcg accgcttggc ggactcttct
     3721 ttggtatgtt gggcgataaa tatggtcgcc agaagatcct cgctatcact attgtgatta
     3781 tgtcgatcag tacgttctgt attggcttaa taccgtccta cgacacgatt ggtatttggg
     3841 caccgattct gctgttgatc tgtaagatgg cacaaggttt ctcggtcggc ggtgaatata
     3901 ccggggcgtc gatatttgtt gcggaatact cccctgaccg taaacgtggc tttatgggca
     3961 gctggctgga cttcggttct attgccgggt ttgtgctggg tgcgggcgtg gtggtgttaa
     4021 tttcgaccat tgtcggcgaa gcgaacttcc tcgactgggg ctggcgtatt ccgttcttta
     4081 ttgctctgcc gttagggatt atcgggcttt acctgcgcca tgcgctggaa gaaactccgg
     4141 cgttccagca gcatgttgat aaactggaac agggcgaccg cgaaggtttg caggatggcc
     4201 cgaaagtctc gtttaaagag attgccacta aatactggcg cagcctgttg acatgtattg
     4261 gtctggtaat tgccaccaac gtgacttact acatgttgct gacctatatg ccgagttatt
     4321 tgtcgcataa cctgcattac tccgaagacc acggggtgct gattattatc gccattatga
     4381 tcggtatgct gtttgtccag ccggtgatgg gcttgctgag tgaccgtttt ggccgtcgtc
     4441 cgtttgtgct acttggtagt gttgccctgt ttgtgttggc gatcccggcg tttattctga
     4501 ttaacagtaa cgtcatcggc ctgatttttg ccgggttact gatgctggcg gtgatcctta
     4561 actgctttac gggcgttatg gcttctacct tgccagcgat gttcccgacg catatccgtt
     4621 acagcgcgct ggcggcggca tttaatattt cggtgctggt tgccggtctg acgccaacac
     4681 tggcggcctg gctggtcgaa agctcgcaga atctgatgat gcctgcctat tacctgatgg
     4741 tagtggcggt gattggttta atcaccggcg taaccatgaa agagacggca aatcgtccgt
     4801 tgaaaggtgc gacaccggcg gcgtcagata tacaggaagc gaaggaaatt ctcgtcgagc
     4861 attacgataa tatcgagcag aaaatcgata atattgacca cgagattgcc gatttgcagg
     4921 cgaaacgtac ccgcctggtg cagcaacatc cgcgaattga tgaataagct gaaacggatg
     4981 gcctgatgtg acgctgtctt atcaggccaa ttgaactctt aaggttcact taatctctga
     5041 cgcgcatact ctcctccagg ttaacggagg agagtgcaat gaaaaaccgt gtttatatga
     5101 aagtttaact accgtgttca gcgtgctggt ggtcagcagc tttctttata tctggtttgc
     5161 cacgttctga tctttcttca gccgtaccca ggcccgcgtg ccggaagtct cttgccggtt
     5221 ttgcaggaaa aactgcccgt gatgcaactg tgtaatgcgg ctgacaatac ttaaccccag
     5281 accaatcccg ccataacggc tgtccatacg tacaaacgct ttactcaact ccccgcattt
     5341 actctcatca atacctggtc cttcatcttc aactgccatg accgctccgc catcttcttg
     5401 cagcttaatc ataatgttgc tgccttgcgg gctgtaacga tgggcgtttt ctaccaggtt
     5461 tcgcaataac atccgcagca gggttgcatc accctgaacg gtgatgtcgg cggcgctctc
     5521 tggcaatagc agggtttgct gtcgctggtc gagcatggta ctgagttcgt catacgaggg
     5581 gagaatgaca tcttccagca gttttacatg ttgataatta ccggaagaaa atgactgtcc
     5641 ggcacgcgcc agttgcagca gctgggagac gctctccatc atctgatcaa gccgtgccac
     5701 taacggtgct acatcaatgt gatgcgtttt cgccagcagt tccagatgca aacgcacccc
     5761 cgccagtggc gttcgcagtt cgtgcgcgac gtcagcggta aacaaccttt cgttatccag
     5821 cgtgctggtc aggcgactga ccagatcgtt caacgccgaa accaccgctt cgatttcgag
     5881 ggtggcgctg tgaatggcaa tgggcgttaa gttgtcggca gtgcgcgctt ccagctcttt
     5941 ttgcagctcc gccagcgggc gggtgatgcg gcgtaccgcc tgatagcaga taaatagcgt
     6001 caggctgacc ataaagacgc cggggacaat caggctggcg accgcctcgc ggatctcacg
     6061 catgatgtgg cgatcgttgt tgcgattgtc gcgtagcgcc tgctcaaaca gctgaatctg
     6121 ctcggtactt tcatgccata gccagaagac gctgatcagc tcaaacacca acaaaatggc
     6181 cccgatagtc aatatcagcc gttggcgcag cgatattggt cggcgcagaa aacgcatcag
     6241 attcaattag ttttcctcat tcgcgaccag catatagcca aagccgcgca cggtgcggat
     6301 acgggatttg cccactttgt cgcgcagatt gtggatatgc acttccaggg tgttggtcga
     6361 gggttcattg tcccagttat agatgtcgtt gtagagaatt tcccgatgta ccggactgcc
     6421 tgctttgagc attaaccgtg acagcagagc atattctttg ggcgtcagaa tcaactcttc
     6481 accgcccatc catacctgac ggcgacccat attcagcgtc agattgccaa caatcagctc
     6541 actttcgccc tgattattat ggcgtcgtag cagggcgcgg atacgggcgt gtaactcttc
     6601 cagcgcaaaa ggcttcacca gatagtcatc ggcaccgaca tccagcccgg cgattttgtc
     6661 ggtcagcgta tcgcgagcgg tgaggatcag taccggcagg gtatattttt tctgccggat
     6721 acgggcgaga aaatgcagtc catcttcgtc gggtaaccct aaatccagta ccaccaggct
     6781 gtaatgaccg gcttcaaggc tttgttccgc catccgcgcg gttgtcacgc catcgcacgc
     6841 gtagccttcg gtttgcgccg ccagaatcag tccctgcaat aacagcgtat cgtcttcaac
     6901 aatcagaatt ttcatttact cactctcctg caagtttgca gaatatcatc cgcagcctgg
     6961 taatacttcg tctcaacgcc agttaatccc aatagcgtgg agaataaatt gtcttgtgaa
     7021 tagtgttgcg tttgcgcctg tttttgcagg cagttctggt caacctgata ccgtttttga
     7081 taatcctccg acagccacag cagcatcggc acctgttttt ggctatccgg ggcgatggca
     7141 taaggcagac cgtgcagata gatgccattt tcacctaacg attcaccgtg gtcagaaaga
     7201 taaaccaggc tggtggtaaa tttatcctga tgttctttca gcagattaat cgctttatca
     7261 acaatatagt cgacgtaaac cagcgtgttg tcgtaagtgt tcaccagttg ctctttggta
     7321 caggtctgga tctcattggt gtcgcaggtt ggggtaaatt tcctgaactg cggcggatag
     7381 cggttgtaat aggtcggacc gtggctgccg atggtgtgta agacaatcac gccatcaccc
     7441 tgcaggttat tgatgtactc ttccagcccg tggaacagca cttcgtcata gcattcgccg
     7501 ttgatgcact gaccaggcag attcagcgcg gtgacgttct ggtgaggcac gcggtcgcag
     7561 gcacctttac agccgccatc gttgtcattc cacagcacgt tgatgcccgc tcgctgaatg
     7621 atatccagca cgccttcctg gtgctgtgcc agctcttctt tgtaatgctc acgcggcata
     7681 tccgagaaca tgcacggtac tgaaactgcc gttgccgtgc cgcaagatac ggtattaggg
     7741 aaatagacca cgttatcttt cgccagccgc gggttagttt cacgcgggta gccgttgagg
     7801 gagaagttct ccgcccgcga ggtttcgccg acaatcagga tggtcaaatt tttacgtttt
     7861 tcgttctgca ttaacgggtt gcggtgggcg tcttcaccaa ttcgcaccag cggtagattt
     7921 gccagtcgct gatgggagta ccatgaccag ctggcaacaa tgctgttaga ggggcttaag
     7981 gatttcacca gctctttgtt attgcggaac aacgaggcgt agtctttata aaacagtgcg
     8041 gcgaccagca aaatcagtag tacagaaacc agaatattgg ctccacggaa aagaacactg
     8101 cgcagacgcg aggtggcagg tttgattttt atccagcagg caatcagcgc agcaagcacg
     8161 ccgctgaatc ccagcgttaa taacatttgc ggtgtcatca gcgcataact ttctgccgga
     8221 gtggtatcaa taatattggc aatcatcgag cggtcgatga cgatgccgta agtcattatg
     8281 aaatattgtg cagccgcgcc aaccagaata aacaggcagg ccagtggtcg attaagccat
     8341 aagaaagagc ttagtgtcag gacaatatta atcacgctga aagcgacgac cggcatcgac
     8401 aagaaaacca gtacgttatg cagcgaatcc agcggcagcg cctgcaacac ctgtttaaaa
     8461 aaggcaatat tcaggcagat agagatataa aaagcggcca acaatagcca ggcgagtaaa
     8521 ttcaaagagg gtctttttag taggcgcttc aacatcacgg tgtttccatc gaacaaagtg
     8581 cgcatatgct cgcaaagcaa aattaagcca accttaagtt cttaaggttg gcttttatgt
     8641 ttgctggatt aaggggaatt aatctttgct tattggtgca tctaagggat acgggttttt
     8701 atgtagccgg ttgtaattca gcgcatacat ggcggtgatg accatcaggg tgacaaatga
     8761 ccacataacc tctttcgctc cggaccccac cacggcccag atgcagtaga ggaaggcaat
     8821 ggtagtaact gccagatatg ccgggcgtgc tttaccaaag tgaccgtgtc cgagcagcag
     8881 taacgccgca caggtgtaaa gatatggcac cagtgtaaag atgaccgaga cggaagaaac
     8941 cagaccgaac tctttggtcg cgtttggtga aatgctgctg agctggaaga tggtcatcaa
     9001 aataccgacg ataatcagcc ccgccactgg cgtacccgct ttatttacac gggcaaaaat
     9061 cggtgggaac agtccgtcat cggcagcggc tttcgccgtt tgacccgcca gcaacgtcca
     9121 gccgcccagt gaacctaagc aacccgcagc tgcgcagaag gaaacaatgg ccccgggggt
     9181 gtcacccaac gccatccgtg cggcatcacc gaatggcgaa gcagaaacgc gcagtgcggc
     9241 attagggatc atccccataa tcgcggtggt agaaagtaca tagcaaacgg cggcaatcaa
     9301 taccccacca atggtggcga tagggacatt gcgtttcggg tttttcacca cacctgcggc
     9361 aacggaggca ctttccacac cgatgaacga ccacagcgta acgttaaggg tactttgaat
     9421 tgcaccgaag gtacccaggc cgctgacgtt ccatgccgcc atataggttt caccacggaa
     9481 ccagaaccag ccaaatacgg caatcccgac gatggggatc agcgccagca cggtggcaac
     9541 tgcctgcaca cgggtgatca ttttcggacc aacaatgttc agcaggacga agatccacag
     9601 caccacgacg caggtgatgg ttaataccaa tggatctttc agaatcggga agaagtaact
     9661 taaatatcct acgccaatga ccaccatggc gatattgccg atccagcagg ccagccagta
     9721 gaggacgttg gtttgataac cgagaaacgg gccaaagcag cggcgggcgt aagcgtaaga
     9781 accaccagga cttgggtcga ggaacgacat tttggcgtat accatcgaga gccccagtgc
     9841 accgataatc gttaccaacc atccataaat ggcaatcccg ccagtggagg ccaggtttgc
     9901 aggtaacaga aaaacacctg accccataat attccccgac accatcaggg tgacggggat
     9961 taagcccact ttgtgagcat cagcatccga agacataatt aaactcctgc gaaggcgagc
    10021 ttcgtgacaa taaattacgt catatcatac gcctgcattc gatagttgaa ttattatcga
    10081 ccgggttatc gctgatgccg ttattaatat agctcttcgt taaggttaaa aatcaggcga
    10141 cagtgcgttc tctgtgctga ctaacataat gcagcggcgt cataccgtag aagtctttaa
    10201 agacagaaat aaagtacgac gtactgttgt agccgcagga ctgtgatacc tgagagatat
    10261 ttttaccgtc catcattaat tcatttacgg cataacgcat gcggcaggtg gtgattattt
    10321 ggctataact ggtgttttcg cttttcagct ttttctttaa cagacttggg ctaagacata
    10381 aacaactggc taccatacta agattccagt ctttgtgaat atcgctttca ataatttgat
    10441 aaacgctgtc acttacacaa ttacgtaaca tatacatcat taatgaaagg aattttttac
    10501 tgtcgagaaa acgggatagc accgtgaata ataatgcgcg tgttcgttcc ttttctgact
    10561 cagtttctgc gggcatcatg ctgtgttgcg cggcaacgcg aaagacgtct ggcgtcaggc
    10621 atggcagggt gagtatgggc gtagcgctac gttgccatac tggtatttgc gagagatctt
    10681 tattcagaaa ccggaggtaa tctttgatga tgtcgtgact aatatgagca accaaagtgt
    10741 tgttaagaga ggagacgtcg ataatattat tttcacaatt taataacgcc atatggttag
    10801 ctttaaggct aataggttct ttcccattca cccttatcca gacatctttt tcagtcaata
    10861 aaacaataca aggttggtcg ctgcaaatcc tcatacatgt actcctgagt gcgaataaaa
    10921 gaccagttct cgctaaagca aagcgatacc gagaaatggt tgtataattg tatgacgaat
    10981 ataaacatgc ataaaaataa cgagttttac taatattaca aatttttata gtaatttctc
    11041 atgtacatat ttttttacag ggtaaataac aggcaaaaaa aagcgcgggg tggggccgcg
    11101 caaaaagtat gacatttttg tttgcagcaa tttttatgaa aacgttgcca tactcaaaag
    11161 gcctaaggcc aggcggcgta atgttattta aacaattacg ccttcagcgg aatagtggtt
    11221 acgctttcac acacatagcg tggtaaatac cgtcaataat ttcagtccct tcagtttcgt
    11281 gttcaaatcc agggaaatgg tggtcccagg attgcagcga gcgtaaataa cttacttgcg
    11341 gactgttttt atcgccgaag ttttcaccag acagcagcat cgggattcct ggcggatatg
    11401 ggataactga gtttgccgcg atgcgtcctg gcagattttc aatggatacc agttcgacat
    11461 tgttgtcgac aatcgcgttg tacgcttcac gcggggtgat ttccgccacc ggcaggccgg
    11521 aataggcttc gttcaaccgt gcgctagggt tgttttcttt cagccaggca aacatggtgt
    11581 cacccagatc gtgaatcccc atgttcgcgt aagtgtcagg atattgttca acaagttccg
    11641 gcatcacctg cgccagcggt gtgttggcgt catagtggcg tttgaaggag caaagggtgt
    11701 taaccagagt cccccatttc ccacgggtta cgcccataga gaacaggaac ataatttgga
    11761 agtcagtggt gcgggtaggc acaatgccgt ggcgaccaag ccaggcagtg accagcgccg
    11821 ccggaacacc ggtttcttcc agttcaccat cttcacccat tcccggagca aggatgctga
    11881 ctttaatcgg gtcgagcata ctccagttat ccggaatatc tttgaagccg tgccagcttt
    11941 cgcccggatg cattacccag tagtcctgaa cggtggtcag cagtttggtt ggtgcgtcag
    12001 caaagtcata ggttttgccg gtttgtgggt cggtgacgac ttctttgttc cacggtttga
    12061 agaaccagct accgtcagcg gtgaactctt tatacagccg cgccatcgcc tgacggaaat
    12121 caaccgcttc gtcgatcact tcctgcgtca gtgacaggcc gctgttgccg tccatcatcg
    12181 acaccgccac gtcgttggat gcgcagatgg catacagcgg ggaggtggtg gcatgcatca
    12241 tgtaggcctg gttgaagcgg gagaagttaa tcgccccacg accttcacgt acatgaatat
    12301 aagaagcctg tgaaagcgca ttcagcagtt tgtgggtgga gtgggtggcg aaaacggtag
    12361 gaccgttgtg atcgccaggt tcgccgcgca tggcatagtg atcggcatag atcgggttga
    12421 aacgtgcata gccgtaccag gcttcgtcaa agtgcagacg atcggaggtt ttttccagca
    12481 gatcctgcgc ttctttagcg ttataacaca cgccgtcata ggtgcagttg gtcaccacgc
    12541 agtaagacgg tttttgcccg gctttgtctt tagtcagcgg gctttcactg attttcttct
    12601 gcaaggtttc aggttgcatt tcctgcggat agattggccc gataatgccg tagcggttgc
    12661 ggcttggcac catgtagacc ggtttcgcgc ctgtcagcat caagccttgt tcgatggatt
    12721 tatggcagtt acggtcaacg accacgacat cgttatcggt catgcaagcc tgcatgatgg
    12781 tgcggttaga gccggaagta ccgacgacta ccgaccagga gcgatcggca ccaaatacgc
    12841 gtgcggcata tttttcgctt tcgccaaatg cgccagtatg gtcaagcaaa gaaccgaggg
    12901 aagttcgttc gatgcccata tcggtgcgga acagattttc tccatagtag tcatggtaga
    12961 aacgtccggc gggtgttttg gtaaaaccaa cgccgccctg gtggcctggc gctgcccagg
    13021 aatattcatg gatgtcgcta tacttcatca gcgcgctgaa cagtggcggc aacagctgct
    13081 ggcggtagcg ggtcatcgcg gcaacggcgc gtccggcgat aaagtcggtg gtatcttcca
    13141 gaatccaggc gaattcatcg acaagctcca gcaggtcgcg atccattgcg gcgagggctt
    13201 tttcccgatc gcccaacagg aagaccggca cgttttgttg gcgctcatga agcttaccga
    13261 tcaattgtct gacgttttga tgttcgtccg gatgttccat ttgatagctg aacatcaggc
    13321 agtcaatggc ttcgtttgaa gagagaatgg caaaaccatc atcaaaggag gtggatttaa
    13381 tcacggtaac attttgctgg cttaaagcat ctgccagacg ctcaacggcg ttaccgaccc
    13441 aggtgtcttg atggagaaac tcgctttcaa caattaatac tttcatcatt gcttacccgg
    13501 ttatgaagga atcttcgttg cgggcaagta tcttccggcc ttttcgggct gtaaagcgcg
    13561 tgaaaaacaa acatttttga aatatttatt tttataaaca atgcgttatg tgattatttt
    13621 aaattatttt cactgcttgt tatcgacggc taaactattt ttttggctga tactgatatc
    13681 gtctttagcc gggaaacgtc tggcggcgct gttggctaag tttgcggtat tgttgcggcg
    13741 acatgccgac atatttgccg aacgtgctgt aaaaacgact acttgaacga aagcctgccg
    13801 tcagggcaat atcgagaata cttttatcgg tatcgctcag taacgcgcga acgtggttga
    13861 tgcgcatcgc ggtaatgtac tgtttcatcg tcaattgcat gacccgctgg aatatcccca
    13921 ttgcatagtt ggcgttaagt ttgacgtgct cagccacatc gttgatggtc agcgcctgat
    13981 catagttttc ggcaataaag cccagcatct ggctaacata aaattgcgca tggcgcgaga
    14041 cgctgttttt gtgtgtgcgc gaggttttat tgaccagaat cggttcccag ccagagaggc
    14101 taaatcgctt gagcatcagg ccaatttcat caatggcgag ctggcgaatt tgctcgttcg
    14161 gactgtttaa ttcctgctgc cagcggcgca cttcaaacgg gctaagttgc tgtgtggcca
    14221 gtgatttgat catcatgccg tgagtgacgt ggttaatcag gtctttatcc agcggccagg
    14281 agagaaacag atgcatcggc agattaaaaa tcgccatgct ctgacaggtt ccggtatctg
    14341 ttagttggtg cggtgtacag gcccagaaca gcgtgatatg accctgattg atattcactt
    14401 tttcattgtt aatcaggtat tccacatcgc catcgaaagg cacattcact tcgacctgac
    14461 catgccagtg gctggtgggc atgatatgcg gtgcgcgaaa ctcaatctcc atccgctggt
    14521 attccgaata cagcgacagc gggctgcggg tctgtttttc gtcgctgctg cacataaacg
    14581 tatctgtatt catggatggc tctctttcct ggaatatcag aattatggca ggagtgaggg
    14641 aggatgactg cgagtgggag cacggttttc accctcttcc cagagaggcg aggggagcgt
    14701 ccgagcatca tgaggccgaa aactctgctt ttcaggtaac ttattaccat aaactcagat
    14761 ttactgctgc ttcacgcagg atctgagttt atgggaatgc tcaacctgga agccggaggt
    14821 tttctgcaga ttcgcctgcc atgatgaagt tattcaagca agccaggaga tctgcatgat
    14881 gactgcaccc aaaattacat ttatcggcgc tggttcgacg attttcgtta aaaatattct
    14941 tggtgatgtg ttccatcgag aggcgctgaa aacggcgcat attgccctga tggacattga
    15001 tcccacccgc ctggaagagt cgcatattgt ggtgcgtaag ctgatggatt cagcaggggc
    15061 cagcggcaaa atcacctgcc acacccaaca gaaagaagcc ttacaggatg ccgattttgt
    15121 cgtggtggca tttcagattg gcggttatga accttgcacg gtgactgatt tcgaggtctg
    15181 taagcggcat ggtctggaac aaaccattgc cgatacgttg gggccgggcg gtattatgcg
    15241 cgcgctacgt accattccgc atctgtggca aatttgcgag gacatgacgg aagtctgccc
    15301 cgatgccaca ttgctcaact acgttaaccc aatggcgatg aatacctggg cgatgtatgc
    15361 ccgctatccg catatcaaac aggtagggct gtgccattcg gtgcagggaa cggcggaaga
    15421 gctggcgcgt gacctcaata tcgatccgtc cacgctgcgt taccgttgcg ctggtatcaa
    15481 ccatatggcg ttttacctgg aactggagcg taaaaccgcc gacggcagtt acgtgaatct
    15541 ctacccggaa ctgctggcgg cttatgaagc agggcaggca ccgaagccac atattcatgg
    15601 taatactcgc tgccagaata ttgtgcgcta cgaaatgttc aaaaagctgg gctacttcgt
    15661 cacggaatcg tcagaacatt ttgccgaata tacgccgtgg tttattaagc caggtcgtga
    15721 ggatttgatt gagcgttata aagtaccgct ggatgagtac ccgaaacgct gcgtcgagca
    15781 gctggcgaac tggcataaag agctggagga gtataaaaac gcctcccgga ttgatattaa
    15841 accgtcacgg gaatatgcca gcacaatcat gaacgctatc tggactggcg agtcgagtgt
    15901 gatttacggc aacgtccgta acgatggttt gattgataac ctgccacaag gatgttgcgt
    15961 ggaagtagcc tgtctggttg atgctaatgg cattcagccg accaaagtcg gtacgctacc
    16021 ttcgcatctg gccgccctga tgcaaaccaa catcaacgta cagacgctgc tgaccgaagc
    16081 tattcttacg gaaaatcgcg accgtgttta ccacgccgcg atgatggatc cgcatactgc
    16141 cgccgtgctg ggcattgacg aaatatatgc tcttgttgac gacctgattg ccgcccacgg
    16201 cgactggctg ccaggctggt tgcaccgtta aaacgcgact aaacgctact gcgccggggg
    16261 atttattccg gcgcacacct ctgacgatac caataacaga aggcgggcgt tggtaacagc
    16321 gacccgatac cctatgagca tttcaatgac tacaaaactc agttatggat ttggagcgtt
    16381 cgggaaggat tttgcgatcg gcattgtgta tatgtacctc atgtattact acaccgatgt
    16441 cgttgggctg tctgtgggtt tggtcggtac tttgtttctg gtggcgagga tctgggatgc
    16501 tattaacgat ccgattatgg g
//
LOCUS       DAJTNN010000087        16479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTNN010000087 DAJTNN010000000
VERSION     DAJTNN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.087"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(124..1407)
                     /gene="gltA"
                     /locus_tag="OO150_002444"
     CDS             complement(124..1407)
                     /gene="gltA"
                     /locus_tag="OO150_002444"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5516550.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1613..1720)
                     /locus_tag="OO150_002445"
     CDS             complement(1613..1720)
                     /locus_tag="OO150_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516551.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2101..2505
                     /gene="sdhC"
                     /locus_tag="OO150_002446"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2101..2505
                     /gene="sdhC"
                     /locus_tag="OO150_002446"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5516552.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2499..2846
                     /gene="sdhD"
                     /locus_tag="OO150_002447"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2499..2846
                     /gene="sdhD"
                     /locus_tag="OO150_002447"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5516553.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2846..4612
                     /gene="sdhA"
                     /locus_tag="OO150_002448"
     CDS             2846..4612
                     /gene="sdhA"
                     /locus_tag="OO150_002448"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5516554.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4628..5344
                     /gene="sdhB"
                     /locus_tag="OO150_002449"
     CDS             4628..5344
                     /gene="sdhB"
                     /locus_tag="OO150_002449"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5516555.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5645..8446
                     /gene="sucA"
                     /locus_tag="OO150_002450"
     CDS             5645..8446
                     /gene="sucA"
                     /locus_tag="OO150_002450"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5516556.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8461..9678
                     /gene="odhB"
                     /locus_tag="OO150_002451"
     CDS             8461..9678
                     /gene="odhB"
                     /locus_tag="OO150_002451"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5516557.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9954..11120
                     /gene="sucC"
                     /locus_tag="OO150_002452"
     CDS             9954..11120
                     /gene="sucC"
                     /locus_tag="OO150_002452"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5516558.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11120..11989
                     /gene="sucD"
                     /locus_tag="OO150_002453"
     CDS             11120..11989
                     /gene="sucD"
                     /locus_tag="OO150_002453"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5516559.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12093..12815)
                     /gene="mngR"
                     /locus_tag="OO150_002454"
     CDS             complement(12093..12815)
                     /gene="mngR"
                     /locus_tag="OO150_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5516560.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12984..14900
                     /gene="mngA"
                     /locus_tag="OO150_002455"
     CDS             12984..14900
                     /gene="mngA"
                     /locus_tag="OO150_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5516561.1"
                     /translation="MNLTTLTHRDALRLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVPACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMVNGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14918..>16479
                     /locus_tag="OO150_002456"
     CDS             14918..>16479
                     /locus_tag="OO150_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5516562.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPTDENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVL"
BASE COUNT         3954 a         4132 c         4489 g         3904 t
ORIGIN      
        1 ttttagaata tatgaatgtc cgatagtgga ttttattttt acaatttttt gtgtttaggc
       61 atataaaaat taacccgcca tttgaacggc gggttaaaat atttacaact tagcaatcaa
      121 ccattaacgc ttgatatcgc ttttaaagtc gcgtttttca tatcctgtat acagctgacg
      181 cggacgggca atcttcatac cgtcactgtg catttcgctc cagtgggcga tccagccaac
      241 ggtacgtgcc attgcgaaaa tgacggtgaa catggaagac ggaataccca tcgctttcag
      301 gatgatacca gagtagaaat cgacgttcgg gtacagtttc ttctcgataa agtacgggtc
      361 gttcagcgcg atgttttcca gctccatagc cacttccagc aggtcatctt tggtgcccag
      421 ctctttcaga acttcatggc aggtttcacg cattacggtg gcgcgcgggt cgtaattttt
      481 gtacacgcgg tgaccgaagc ccatcaggcg gaaagaatca tttttatctt tcgcacgacg
      541 aacaaattcc ggaatgtgtt taacggagct aatttcttcc agcattttca gcgccgcttc
      601 gttagcacca ccgtgcgcag gtccccacag tgaagcaata cctgctgcga tacaggcaaa
      661 cgggttcgca cccgaagagc cagcggtacg cacggtggag gtagaggcgt tctgttcatg
      721 gtcagcgtgc aggatcagaa tacggtccat agcacgttcc agaatcggat taacttcata
      781 cggttcgcac ggcgtggaga acatcatatt caggaagtta ccggcatagg agagatcgtt
      841 gcgcggataa acaaatggct gaccaatgga atacttgtaa cacatcgcgg ccatggtcgg
      901 cattttcgac agcaggcgga acgcggcaat ttcacgatga cgaggattgt taacatccag
      961 cgagtcgtga tagaacgccg ccagcgcgcc ggtaatacca cacatgactg ccattggatg
     1021 tgagtcgcga cggaaagcgt ggaacagacg ggtaatctgc tcgtggatca tggtatgacg
     1081 ggtcaccgta gttttaaatt cgtcatactg ttcctgagtc ggtttttcac cattcagcag
     1141 gatgtaacaa acttccaggt agttagaatc ggtcgccagc tgatcgatcg ggaaaccgcg
     1201 gtgcagcaaa ataccttcat caccatcaat aaaagtaatt ttagattcgc aggatgcggt
     1261 tgaagtgaag cctgggtcaa aggtgaacac accttttgaa ccgagagtac ggatatcaat
     1321 aacatcttga cccagcgtgc ctttcagcac atccagttca acagctgtgt ccccgttgag
     1381 ggtgagtttt gcttttgtat cagccattta aggtctcctt agcgccttat tgcgtaagac
     1441 tgccggaact taaatttgcc ttcgcacatc aacctggctt tacccgtttg ttatttggct
     1501 cgccgctctg tgaaagaggg gaaaacctgg gtacagagct ctgggcgctt gcaggtaaag
     1561 gatccattga tgacgaataa atggcgaatc aagtacttag caatccgaat tattaaactt
     1621 gtctaccact aataactgtc ccgaatgaat tggtcaatac ttccacactg ttacataagt
     1681 taatcttagg tgaaataccg acttcataac ttttacgcat tatatgcttt tcctggtaat
     1741 gtttgtaaca actttgttga gtgattgtca aattagatga ttaaaaatta aataaatgtt
     1801 gttgtcgtga cctggatcac tgttcaggat aaaacccgac aaactatatg taggttaatt
     1861 gtaatgattt tgtgaacagc ctatactgcc gctaggtctc cggaacaccc tgcaatcccg
     1921 agccacccag cgttgtaacg tgtcgttttc gcatctggaa gcagtgtttt gcatgacgcg
     1981 cagttataga aaggacgctg tctgacccgc aagcagaccg gaggaaggaa atcccgacgt
     2041 ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc cagggaataa taagaacagc
     2101 atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa gacctgttaa tctggaccta
     2161 cagaccatcc ggttccccat cacggcgata gcgtccattc tccatcgcgt ttccggtgtg
     2221 atcacctttg ttgcagtggg catcctgctg tggcttctgg gtaccagcct ctcttcccct
     2281 gaaggtttcg agcaagcttc cgcgattatg ggcagcttct tcgtcaaatt tatcatgtgg
     2341 ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta ttcgccacat gatgatggat
     2401 tttggctatc tggaagaaac attcgaagcg ggtaaacgct ccgccaaaat ctcctttgtt
     2461 attactgtcg tgctttcact tctcgcagga gtcctcgtat ggtaagcaac gcctccgcat
     2521 taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc taccgctatc gtcctgacgc
     2581 tctacatcat ttatatggtc ggttttttcg ccaccagtgg cgaactgaca tatgaagtct
     2641 ggatcggttt cttcgcctct gcgttcacca aagtgttcac cctgctggcg ctgttttcta
     2701 tcttgattca tgcctggatc ggcatgtggc aggtgttgac cgactacgtt aaaccgctgg
     2761 cattgcgcct gatgctgcaa ctggtgattg tcgttgcact ggtggtttac gtgatttatg
     2821 gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc agagaatttg atgcagttgt
     2881 gattggtgcc ggtggcgcag gtatgcgcgc ggcgctgcaa atttcccaga gcggccagac
     2941 ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat accgtttctg cgcaaggcgg
     3001 cattaccgtt gcgctgggta atacccatga agataactgg gaatggcata tgtacgacac
     3061 cgtaaaaggg tcggactata tcggtgacca ggacgcgatt gaatatatgt gtaaaaccgg
     3121 gccggaagcg attctggaac tcgaacacat gggcctgccg ttctcgcgtc ttgatgatgg
     3181 tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac ttcggcggcg agcaggcggc
     3241 acgcactgcg gcggcggctg accgtaccgg tcacgcactg ttgcacacgc tttatcagca
     3301 gaacctgaaa aaccacacca ccattttctc cgagtggtat gcgctggatc tggtgaagaa
     3361 ccaggatggc gcggtggtgg gttgtactgc actgtgcatc gaaactggtg aagtggttta
     3421 tttcaaagcc cgcgctaccg tgctggcgac tggcggggca gggcgtattt atcagtccac
     3481 cactaacgcc cacattaaca ctggcgacgg cgtcggcatg gctatccgcg caggcgtacc
     3541 ggtgcaggat atggaaatgt ggcagttcca cccgaccggt attgccggtg cgggcgtact
     3601 ggtcaccgaa ggttgccgtg gtgaaggcgg ttatctgctg aacaaacatg gcgaacgctt
     3661 tatggagcgt tatgcgccga acgccaaaga cctggcgggc cgtgatgtgg tggcgcgttc
     3721 catcatgatc gaaatccgtg aaggtcgcgg ctgtgatggt ccgtgggggc cacacgcgaa
     3781 actgaaactc gaccacctgg gtaaagaagt tctcgaatcc cgtctgccgg gcatcctgga
     3841 actctcccgt accttcgctc acgtcgatcc ggtgaaagag ccgatcccgg ttatcccaac
     3901 ctgtcattac atgatgggcg gtattccgac caaagtgacc ggtcaggcgc tgactgtgaa
     3961 tgagaaaggc gaagatgtgg ttgttccggg actgtttgcc gttggtgaaa tcgcttgtgt
     4021 atcggtacac ggcgctaacc gtctgggcgg caactcgctg ctggacctgg tggtctttgg
     4081 tcgtgcggca ggtctgcatc tgcaagagtc tatcgccgag cagggcgcat tgcgcgatgc
     4141 cagcgagtct gatgttgaag catctctgga tcgtctgaac cgctggaaca ataatcgtaa
     4201 cggtgaagat ccggtggcga ttcgtaaagc gctgcaagaa tgtatgcagc ataacttctc
     4261 ggtcttccgt gaaggtgatg cgatggcgaa agggcttgag cagttgaaag ttatccgcga
     4321 gcgtctgaaa aatgcccgtc tggatgacac ttccagcgag ttcaacaccc agcgcgttga
     4381 gtgcctggaa ctggataacc tgatggaaac ggcgtatgca acggctgttt ctgccaactt
     4441 ccgtaccgaa agccgtggcg cgcatagccg cttcgacttc ccggatcgtg atgatgaaaa
     4501 ctggctgtgc cactccctgt atctgccaga gtcggaatcc atgacgcgcc gaagcgtcaa
     4561 catggaaccg aaactgcgcc cggcattccc gccgaagatt cgtacttact aatgcggaga
     4621 caggaaaatg agactcgagt tttcaattta tcgctataac ccggatgttg atgatgctcc
     4681 gcgtatgcag gattacaccc tggaagcgga agaaggtcgc gacatgatgc tgctggatgc
     4741 gcttatccag ctgaaagaga aagatcccag cctgtcgttc cgccgctcct gtcgtgaagg
     4801 tgtgtgcggt tccgacggtc tgaacatgaa cggcaagaat ggtctggcct gtattacccc
     4861 gatttcggca ctcaaccagc cgggcaagaa gattgtgatt cgcccgctgc caggtttacc
     4921 ggtgatccgc gatttggtgg tagacatggg acaattctat gcgcaatatg agaaaattaa
     4981 gccttacctg ttgaataatg gacaaaatcc gccagctcgc gagcatttac agatgccaga
     5041 gcagcgcgaa aaactcgacg ggctgtatga atgtattctc tgcgcatgtt gttcaacctc
     5101 ttgtccgtct ttctggtgga atcccgataa gtttatcggc ccggcaggct tgttagcggc
     5161 atatcgtttc ctgatcgata gccgtgatac cgagactgac agccgcctcg acggtttgag
     5221 cgatgcattc agcgtattcc gctgtcacag catcatgaac tgcgtcagtg tatgtccgaa
     5281 ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg atgttgttgc aacgtaatgc
     5341 gtaaaccgta ggcctgataa gacgcgcaag cgtcgcatca ggcaaccagt gccggatgcg
     5401 gcgtgaacgc cttatccggc ctacaagtca ttacccgtag gcctgataag cgcagcgcat
     5461 caggcgtaac aaagaaatgc aggaaatctt taaaaactgc ccctgacact aagacagttt
     5521 ttaaaggttc cttcgcgagc cactacgtag acaagagctc gcaagtgaac cccggcacgc
     5581 acatcactgt gcgtggtagt atccacggcg aagtaagcat aaaaaagatg cttaagggat
     5641 cacgatgcag aacagcgctt tgaaagcctg gttggactct tcttacctct ctggcgcaaa
     5701 ccagagctgg atagaacagc tctatgaaga cttcttaacc gatcctgact cggttgacgc
     5761 taactggcgt tcgacgttcc agcagttacc tggtacggga gtcaaaccgg atcaattcca
     5821 ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac gcttcacgtt actcttcaac
     5881 gatctccgac cctgacacca atgtgaagca ggttaaagtc ctgcagctca ttaacgcata
     5941 ccgcttccgt ggtcaccagc atgcgaatct cgatccgctg ggactgtggc agcaagataa
     6001 agtggccgat ctggatccgt ctttccacga tctgaccgaa gcagacttcc aggaaacctt
     6061 caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa ctcggcgagc tgctggaagc
     6121 cctcaagcaa acctactgcg gcccgattgg tgccgagtat atgcacatca ccagcaccga
     6181 agaaaaacgc tggatccaac agcgtatcga gtctggtcgc gcgactttca atagcgaaga
     6241 gaaaaaacgc ttcttaagcg aactgaccgc cgctgaaggc cttgaacgtt acctcggcgc
     6301 aaaattccct ggcgcaaaac gcttctcgct ggaaggcggt gacgcgttaa tcccgatgct
     6361 taaagagatg atccgccacg ctggcaacag cggcacccgc gaagtggttc tcgggatggc
     6421 gcaccgtggt cgtctgaacg tgctggtgaa cgtgctgggt aaaaaaccgc aagacttgtt
     6481 cgacgagttc gccggtaaac ataaagaaca cctcggcacg ggtgacgtga aataccacat
     6541 gggcttctcg tctgacttcc agaccgatgg cggcctggtg cacctggcgc tggcgtttaa
     6601 cccgtctcac cttgagattg taagcccggt agttatcggt tctgttcgtg cccgtctgga
     6661 cagacttgat gagccgagca gcaacaaagt gctgccaatc accatccacg gtgacgccgc
     6721 agtgaccggg cagggcgtgg ttcaggaaac cctgaacatg tcgaaagcgc gtggttatga
     6781 agttggcggt acggtacgta tcgttatcaa caaccaggtt ggtttcacca cctctaatcc
     6841 gctggatgcc cgttctacgc cgtactgtac tgatatcggt aagatggttc aggccccgat
     6901 tttccacgtt aacgcggacg atccggaagc cgttgccttt gtgacccgtc tggcgctcga
     6961 tttccgtaac acctttaaac gtgatgtctt catcgacctg gtgtgctacc gccgtcacgg
     7021 ccacaacgaa gccgacgagc cgagcgcaac ccagccgctg atgtatcaga aaatcaaaaa
     7081 acatccgaca ccgcgcaaaa tctacgctga caagctggag caggaaaaag tggcgacgct
     7141 ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg ctggatgctg gcgattgcgt
     7201 agtggcagag tggcgtccga tgaacatgca ctctttcacc tggtcgccgt acctcaacca
     7261 cgaatgggac gaagagtacc cgaacaaagt tgagatgaag cgcctgcagg agctggcgaa
     7321 acgcatcagc acggtgccgg aagcagttga aatgcagtct cgcgttgcca agatttatgg
     7381 cgatcgccag gcgatggctg ccggtgagaa actgttcgac tggggcggtg cggaaaacct
     7441 cgcttacgcc acgctggttg acgaaggcat tccggttcgc ctgtcgggtg aagactccgg
     7501 tcgcggtacc ttcttccacc gccacgcggt gatccacaac cagtctaacg gttccactta
     7561 cacgccgctg caacacatcc ataacgggca gggcgcgttc cgcgtctggg actccgtact
     7621 gtctgaagaa gcagtgctgg cgtttgaata cggttatgcc accgcagaac cacgcactct
     7681 gaccatctgg gaagcgcagt tcggtgactt cgctaacggt gctcaggtgg ttatcgacca
     7741 gttcatctcc tctggcgaac agaaatgggg ccggatgtgt ggtctggtga tgttgctgcc
     7801 gcacggttat gaagggcagg ggccggagca ctcctccgcg cgtctggaac gttatctgca
     7861 actttgtgct gagcaaaaca tgcaggtgtg cgtaccgtct accccggcac aggtttacca
     7921 catgctgcgt cgtcaggcgc tgcgcgggat gcgtcgtccg ctggtcgtga tgtcgccgaa
     7981 atccctgctg cgtcatccgc tggcggtttc cagcctcgaa gaactggcga acggcacctt
     8041 cctgccagcc atcggtgaaa tcgacgagct tgatccgaag ggcgtgaagc gcgtagtgat
     8101 gtgttctggt aaggtttatt acgacctgct ggaacagcgt cgtaagaaca atcaacacga
     8161 tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg cataaagcga tgcaggaagt
     8221 gttgcagcag tttgctcacg tcaaggattt tgtctggtgc caggaagagc cgctcaacca
     8281 gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg attccgtttg gggcttctct
     8341 gcgttatgca ggccgcccgg cctccgcctc tccggcggta gggtatatgt ccgttcacca
     8401 gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc gaataaataa aggatacaca
     8461 atgagtagcg tagatattct ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc
     8521 gcaacctggc ataaaaaacc cggcgacgca gtcgtacgtg atgaagtgct ggtagaaatc
     8581 gaaactgaca aagtggtact ggaagtaccg gcatcagcag acggcattct ggatgcggtt
     8641 ctggaagatg aaggtacaac cgtaacgtct cgtcagatcc ttggtcgcct gcgtgaaggc
     8701 aacagcgccg gtaaagaaac cagcgccaaa tctgaagaga aagcgtccac tccggcgcaa
     8761 cgccagcagg cgtctctgga agagcaaaac aacgatgcgt taagcccggc gatccgtcgc
     8821 ttgctggctg aacataacct cgacgccagc gctattaaag gcaccggcgt gggtggtcgt
     8881 ctgacccgtg aagatgtgga aaaacatctg gcgaaatccc cggcgaaaga gtctgcaccg
     8941 gcagcggctg ctccggcggc gcaaccggct ctggctgcac gtagtgaaaa acgtgtcccg
     9001 atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc
     9061 atgctgacca cgttcaacga agtcaacatg aagccgatta tggatctgcg taagcagtac
     9121 ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct ttatgtcctt ctacgtgaaa
     9181 gcggtggttg aagccctgaa acgttacccg gaagtgaatg cttctatcga cggcgatgac
     9241 gtggtttacc acaactattt cgacgtcagc atggcggttt ctacgccgcg cggcctagtg
     9301 acgccggttc tgcgtgatgt cgataccctc ggcatggcag acatcgagaa gaaaatcaaa
     9361 gagctggcag tcaaaggccg tgatggcaag ctgaccgttg aagatctgac cggtggtaac
     9421 ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt ctacgccgat catcaacccg
     9481 ccgcagagcg caattctggg tatgcacgct atcaaagatc gtccgatggc ggtgaatggt
     9541 caggttgaga tcctgccgat gatgtacctg gcgctgtcct acgatcaccg tctgatcgat
     9601 ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt tgctggaaga tccgacgcgt
     9661 ctgctgctgg acgtgtagta gtttaagttt cacctgcact gtagacagga taaggcatta
     9721 tcgccttctc cggcaattga agcctgatgc gacgcttaac gcgtcttatc aggcctacgg
     9781 gaagcaccaa tgtaggtcgg ataaggcgct tgcgccgcat ccgacaagcg atgcctgatg
     9841 cgacacttac gcgtcttatc aggcctacag gtgaccgaca atgcccggag gcgatacgaa
     9901 atattcggtc tacggtttaa aagataacga ttgctgaagg atggacagaa cacatgaact
     9961 tacatgaata tcaggcaaaa caactttttg cccgctatgg cttaccagca ccggtgggtt
    10021 atgcctgtac tactccgcgc gaagcagaag aagccgcttc aaaaatcggt gccggtccgt
    10081 gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa agcgggcggt gtgaaagttg
    10141 taaacagcaa agaagacatc cgtgcttttg cagaaaattg gctcggcaag cgtctggtaa
    10201 cgtatcaaac agatgccaat ggccaaccgg ttaaccagat tctggttgaa gcagcgaccg
    10261 atatcgctaa agagctgtat ctcggtgccg ttgtagaccg tagttcccgt cgtgtggtct
    10321 ttatggcctc cactgaaggc ggcgtggaaa tcgaaaaagt ggcggaagaa actccgcagc
    10381 tgatccataa agttgcgctt gatccgctga caggcccgat gccgtatcag ggacgcgagc
    10441 tggcgttcaa actgggtctg gaaggtaaac tggttcagca gttcaccaaa atcttcatgg
    10501 gtctggcgac cattttcctg gagcgcgacc tggcgttgat cgaaatcaac ccgctggtca
    10561 tcaccaaaca gggtgatctg atttgcctcg acggcaaact gggcgctgat ggcaacgcac
    10621 tgttccgcca gcttgatctg cgcgaaatgc gtgaccagtc gcaggaagat ccgcgtgaag
    10681 cacaggctgc acagtgggaa ctgaactacg ttgcgctgga cggtaacatc ggttgtatgg
    10741 ttaacggcgc aggtctggcg atgggtacga tggacatcgt taaactgcac ggcggcgaac
    10801 cggctaactt ccttgacgtt ggcggcggtg caaccaaaga acgtgtaacc gaagcgttca
    10861 aaatcatcct ctctgacgac aaagtgaaag tcgttctggt taacatcttc ggcggtatcg
    10921 ttcgttgcga cctgatcgct gacggtatca tcggcgcggt agcagaagtg ggtgttaacg
    10981 taccggtcgt ggtacgtctg gaaggtaaca acgccgaact cggcgcgaag aaactggctg
    11041 acagcggcct gaatattatt gcagcaaaag gtctgacgga tgcagctcag caggttgttg
    11101 ccgcagtgga ggggaaataa tgtccatttt aattgataaa aacaccaagg ttatctgcca
    11161 gggctttact ggtagccagg ggactttcca ctcagaacag gccattgcat acggcactaa
    11221 aatggttggc ggcgtaaccc caggtaaagg cggtactacc cacctcggcc tgccggtgtt
    11281 caacaccgtg cgtgaagctg ttgctgctac tggcgctacc gcttctgtta tctacgtacc
    11341 agcaccgttc tgcaaagact ccattctgga agctattgat gcaggcatca aactgattat
    11401 caccatcact gaaggcatcc cgacgctgga tatgctgact gtgaaagtga aactggacga
    11461 agccggcgtt cgtatgatcg gcccgaactg cccgggcgtt atcactccgg gtgaatgcaa
    11521 aatcggtatc cagccgggtc acattcacaa accgggtaaa gtgggtatcg tttcccgttc
    11581 cggtacactg acctatgaag cggttaaaca gaccacggat tacggtttcg gtcagtcgac
    11641 ctgtgttggt atcggcggtg acccgatccc aggctctaac tttatcgaca tcctcgaaat
    11701 gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc ggtgagatcg gcggtagcgc
    11761 tgaagaagaa gcggcagcgt acatcaaaga gcacgttacc aagccagttg taggttacat
    11821 cgctggtgtg actgcgccga aaggcaaacg tatgggccac gcgggtgcga tcattgccgg
    11881 tgggaaaggg actgcggatg agaaattcgc tgctctggaa gccgcaggcg tgaaaaccgt
    11941 tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt ctgaaataaa tatctgtaat
    12001 aagaaatagc cctcgccgct tccctctaca ggaatggcga agggctgtcg gtttcgacat
    12061 ggttggccat cgtatgatgg ccttttttgt gcttatcgcg atgattttcg ctgcgctatc
    12121 agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc tatattcaaa cacccgacca
    12181 tcaactaaat atccacgcga tactttttca agaatcggct ttgtctggct gatattaagc
    12241 agacggctca tctcttcggt tggcatcaga ggaatgattt cctgttcgct acgatcgata
    12301 accatcttct tcacttcttc aataaagtga tatttcgaat tttccatgac ctgccaggtg
    12361 agatccggga acaacgcaag cggcatccag gtttcttcca gcgccattgg cttttgcttg
    12421 cgatagcgca cgcgcttcac atgccacaca cgatcctgcg cggtgatttg tagctgttgc
    12481 tgaagaaaat cgtcagccgg aatcacttca aatatcagga cttcactgtg tgtatcgacg
    12541 tgacggtccg acagtttttc atcaaaactg gttaactgaa aaatatcgta attgacccgc
    12601 tcttctttga cgtaagtccc gctgccctga atgctttcga ggatctgctg ctcgactagc
    12661 tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa actctgtttg tagcgctgat
    12721 tcagtgggta acgcatcgcc aggtttaagc tcgccacgcg caatttgttc acgaatgcga
    12781 tcggcaatct gccggtataa gggcttgtgt cccattttta gtatctcatt aatacgaatt
    12841 tagccattat gcccgataaa ttcatcctgt aaataatgca aatacaatac aaataatttc
    12901 aatcaagtga aattgatcat ataatggtat tgttttatcg ggcacactgg cgcgactata
    12961 aaaacgatca agtgaggatc atgatgaatc tgacgactct gacccaccgc gatgcgttgc
    13021 gtctgaatgc gcgctttacc agccgtgaag aggccatcca cgcgttgact caacgtcttg
    13081 ctgctctggg gaaaatttcc agtactgagc aatttctgga agaagtgtat cgccgtgaaa
    13141 gccttggccc gaccgcctta ggtgaagggt tggctgtgcc gcatggcaaa actgctgcgg
    13201 taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc gcttcagtgg gaaggcgttg
    13261 atggcccgga agcagttgat ttagtggtgc tgctggcgat tccccccaat gaagcgggta
    13321 ctatgcatat gcaactgctg acagcgctga ccacgcgcct tgcggatgat gagattcggg
    13381 cgcgtataca gtcggcgacg acgcctgatg agttgctctc ggcgctggat gacaagggag
    13441 gcacgcaacc ttctgcctct ttttccaacg cgccaactat cgtctgcgta ccggcctgtc
    13501 cggcgggtat tgctcacacc tatatggctg cggaatatct ggaaaaagcc ggacgcaaac
    13561 tcggcgtaaa tgtttacgtt gaaaaacaag gcgctaacgg cattgaaggg cgtttaacgg
    13621 cggatcaact caatagtgca accgcctgta tttttgcggc tgaagtcgcc atcaaggaga
    13681 gtgagcgttt taatggcatt cccgcgcttt cagtgcctgt tgccgagccg attcgccatg
    13741 cagaagcgtt gatccaacaa gcgcttaccc tcaagcgtag cgatgagacg cgtaccgtac
    13801 agcaagatac gcaaccggtg aaaagtgtca aaacggagct gaaacaggca ctgttgagcg
    13861 gaatctcttt tgccgtaccg ttgattgtcg cggggggcac ggtgctggcg gtcgcggtat
    13921 tactgtcgca aatcttcggg ctacaagatc tgtttaatga agaaaactcc tggctgtgga
    13981 tgtaccgcaa gctgggcggc gggctgctcg gaattttgat ggtaccggtg ctcgcggcct
    14041 atactgccta ttctctggca gataaaccgg cgttagcgcc aggctttgcg gctggacttg
    14101 ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt tggcggattg atagccggtt
    14161 acttgatgcg ctgggtgaaa aatcacttgc gtcttagcag taaattcaat ggattcctga
    14221 ctttttatct ctacccggtg ctcggtacgt tgggagcggg cagtctgatg ctgtttgtgg
    14281 tgggagaacc tgtcgcatgg atcaataact cgcttaccgc ctggctgaac ggtctgtcag
    14341 gaagtaacgc gctgttgctg ggtgccattt tcggttttat gtgttccttt gatcttggag
    14401 ggccagtgaa caaagccgct tatgcattct gcctgggcgc aatggtgaac ggcgtttacg
    14461 gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc atttaccgta accgcttcca
    14521 cgatgctcgc accgcgcctg tttaaagagt ttgaaattga gaccgggaaa tccacctggc
    14581 tgttagggct ggcaggtatt accgaagggg cgatcccgat ggcgattgaa gatccgctgc
    14641 gggttattgg ttcgtttgtg ctgggctcta tggtaacggg cgctattgtc ggtgcgatga
    14701 atatcggcct ttcgacaccc ggtgccggca ttttctcgct ctttttactt catgataatg
    14761 gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc ggcattggtg ggggctgcaa
    14821 tctcgactgc aattctcctg atctggcggc gtcacgcggt taagcatggc aactatctga
    14881 ctgatggcgt aatgccataa acaaaaagga aacgacgatg aaagcggtat ctcgtgttca
    14941 catcaccccg catatgcact gggatcgaga gtggtatttc accaccgaag agtcacgtat
    15001 tctgctggtc aataatatgg aagagatcct gtgccgactg gaacaggaca acgaatacaa
    15061 atattacgta ctcgacgggc aaacggcgat cctcgaagat tatttcgcgg tgaaaccgga
    15121 aaacaaagac cgtgtgaaga aacaggtaga agccggcaag ttgattatcg gcccctggta
    15181 tacccagacc gataccacga ttgtttctgc ggaatccatc gtccgtaatc tgatgtacgg
    15241 aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata ggttatttac cagattcctt
    15301 tggcatgtcc gggcaactgc cgcatatcta caatggattt ggcattaccc gcaccatgtt
    15361 ctggcgcgga tgttcggagc gccacggtac tgataaaacc gagtttttgt ggcaaagcag
    15421 tgacggtagc gaagtgacgg cgcaggtgct gccgctgggc tacgccatag gtaagtactt
    15481 acctaccgac gaaaacggat tacgtaaacg cctcgacagt tattttgacg tgctggaaaa
    15541 agcgtctgta accaaagaga ttttgctgcc gaatggtcat gaccagatgc cattgcagca
    15601 aaatatcttc gaagtgatgg ataagctacg tgagatctac cctcaacgta agtttgtgat
    15661 gagccgcttt gaagaggtat ttgagaagat cgaagcgcag cgtgaaagtc tggccaccct
    15721 gaaaggtgaa tttatcgatg gcaaatatat gcgcgtacat cgcaccatcg gttccacgcg
    15781 catggatatc aaaattgccc acgcacgtat tgaaaataag attgttaatc tgctggaacc
    15841 gctggcaacg ctggcctgga cgttgggttt tgaataccac cacggcttgc tggagaaaat
    15901 gtggaaagag atcttaaaaa atcatgccca cgacagtatc ggttgttgtt gcagtgacaa
    15961 agttcatcgc gaaattgtcg cccgcttcga actggctgaa gacatggcgg ataatctgct
    16021 gcgtttctac atgcgcaaaa ttgccgataa catgccacag agtgatgccg acaaactcgt
    16081 gctgtttaac ctgatgccct ggccgcggga agaagttatt aacaccactg tgcggctgcg
    16141 cgccagccag tttaatctac gagacgatcg cggtcagcct gtaccgtatt ttattcgcca
    16201 tgcccgtgag atcgatccag gcctaatcga tcggcaaata gttcattacg gtaattacga
    16261 tccctttatg gagtttgata tacagatcaa ccagattgtc ccttctatgg gctatcgcac
    16321 gctttatatc gaagcgaatc agcctggcaa cgtgattgcg gcaaaaagtg acgctgaagg
    16381 gatactggaa aatgctttct ggcaaattgc gctcaatgag gatggttctc tgcaactggt
    16441 agataaagac agcggtgtgc gctatgaccg agtattgca
//
LOCUS       DAJTNN010000088        16297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTNN010000088 DAJTNN010000000
VERSION     DAJTNN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.088"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..2165)
                     /gene="topA"
                     /locus_tag="OO150_002457"
     CDS             complement(<1..2165)
                     /gene="topA"
                     /locus_tag="OO150_002457"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5516563.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLK"
     gene            2545..2796
                     /locus_tag="OO150_002458"
     CDS             2545..2796
                     /locus_tag="OO150_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5516564.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(2832..3881)
                     /gene="sohB"
                     /locus_tag="OO150_002459"
     CDS             complement(2832..3881)
                     /gene="sohB"
                     /locus_tag="OO150_002459"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5516565.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4101..4859
                     /locus_tag="OO150_002460"
     CDS             4101..4859
                     /locus_tag="OO150_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5516566.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            4856..5447
                     /gene="cobO"
                     /locus_tag="OO150_002461"
                     /pseudo
     CDS             4856..5447
                     /gene="cobO"
                     /locus_tag="OO150_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5487..6359)
                     /gene="rluB"
                     /locus_tag="OO150_002462"
     CDS             complement(5487..6359)
                     /gene="rluB"
                     /locus_tag="OO150_002462"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5516567.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(6570..8465)
                     /locus_tag="OO150_002463"
     CDS             complement(6570..8465)
                     /locus_tag="OO150_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCR5516568.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8493..9113)
                     /locus_tag="OO150_002464"
     CDS             complement(8493..9113)
                     /locus_tag="OO150_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5516569.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9110..9991)
                     /gene="yciV"
                     /locus_tag="OO150_002465"
     CDS             complement(9110..9991)
                     /gene="yciV"
                     /locus_tag="OO150_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5516570.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRIGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10128..10172
                     /gene="trpL"
                     /locus_tag="OO150_002466"
     CDS             10128..10172
                     /gene="trpL"
                     /locus_tag="OO150_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5516571.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10149..10242
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10264..11826
                     /gene="trpE"
                     /locus_tag="OO150_002467"
     CDS             10264..11826
                     /gene="trpE"
                     /locus_tag="OO150_002467"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5516572.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            11826..13421
                     /gene="trpD"
                     /locus_tag="OO150_002468"
     CDS             11826..13421
                     /gene="trpD"
                     /locus_tag="OO150_002468"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5516573.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13422..14783
                     /gene="trpCF"
                     /locus_tag="OO150_002469"
     CDS             13422..14783
                     /gene="trpCF"
                     /locus_tag="OO150_002469"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5516574.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFNPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     GLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPTHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            14795..15988
                     /gene="trpB"
                     /locus_tag="OO150_002470"
     CDS             14795..15988
                     /gene="trpB"
                     /locus_tag="OO150_002470"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5516575.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            15988..>16297
                     /gene="trpA"
                     /locus_tag="OO150_002471"
     CDS             15988..>16297
                     /gene="trpA"
                     /locus_tag="OO150_002471"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5516576.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLRDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     A"
BASE COUNT         3931 a         4359 c         4077 g         3930 t
ORIGIN      
        1 attttcaggt gcatttcaga gccacatttt tcacactcaa cgatcgggcc gtcataacct
       61 ttaatgcgga attcgccctc ttcgatctcg taaccgtcgc aggttgggtt attaccacag
      121 acatgcaact tacgtttcgg atcgatgaga tagctgtcca tcgccgtgcc gcatttcggg
      181 caacgacgtt ttgcgcgcag cgcgttggtt tcagcgtctt cgccttccag tacgttcagc
      241 acttcgtttt ccggcaccag gttaatggtg gttttgcaac gctctttcgg cggcagcgca
      301 taaccagaac agccaaggaa taccccggtg ctcgctgtgc gaatccccat tttgcgacca
      361 caagtcgggc agtcaatgct ggtcagaacc atctggttcg ggcgcatacc accctcttcc
      421 ggatcttttt cagctttatc taactgctgg gtgaaatccg agaagaagtg atccagtaca
      481 gctttccact ctgcttcgtg atttgccacc tggtcgaggc tgttttccat ctgtgcggtg
      541 aagtcgtagt tcattaactc gcggaaattt tcttcaaggc gatcggtgac gatttcgccc
      601 attttttccg catagaaacg acgattttct actcgcacgt agccacgatc ctgaatggtc
      661 gaaatgatcg acgcataggt agacggacga ccgataccgc gtttttccag ctctttaacc
      721 agcgatgctt cactgaaacg ggctggcggc ttggtaaagt gctgggctgg tgtaagttca
      781 acgagcgtca gagcatcgcc tttattaact gctggtaaga tgcgatcttc atcgccttta
      841 cgcaacgcag gcatcacttt tgtccagcca tcaaaacgca aaatacgacc tcgtgctttc
      901 aggcggaaat cgcccgcacc aacggtcagc gtcgtggagt catatttcgc tggggtcatc
      961 tggcaggcta cgaactgacg ccagattaac tggtacagtt tctgcgcatc tgcttccata
     1021 tccttcagcg attccgccat cacattgacg tcagaaggac gaatcgcttc gtgcgcttcc
     1081 tgtgagtttt ctttgctggc gtactgattc ggactttccg gcagatattt cttaccaaaa
     1141 ttatcgctga tataaccgcg aaccatattt accgcgtcct gactcaggtt agtggagtcg
     1201 gtacgcatgt aagtgatata gcctgcttca tacaaacgct gcgccatcat catggttttt
     1261 ttcacgccaa atccaagacg ggtgctggca gcttgttgca gcgtagaggt aataaaagga
     1321 gcgccaggtt tactggttgt cggtttgtct tcacgttcca gcacgctgta gcgcgctttt
     1381 tccagcagac ttaccgcagc ctgagtttgt tctttgttga ccggacggaa cggtttgtcg
     1441 ttctgatgag tcacctgtaa cgccaacgct tcaccagatg gcgtggtcgt gctggcatcg
     1501 acttcccaga actcttccgg cacgaacgct ttaatttcac gctcacgctc gaccaccagg
     1561 cgaaccgcca ccgactgcac acgaccggca gacagaccac gagcgatctt tttccatagc
     1621 agcggcgaaa ccatataccc caccacgcgg tccataaagc gacgcgcctg ctgggcatta
     1681 acacgatcaa tattcagctc acccggtttg ttaaatgcct ggcggatcgc gtttttagta
     1741 atttcgttaa acaccactcg gctatagcgc gcatcatcac ccccaatcac ttcccgcagg
     1801 tgccatgcaa tggcttcccc ttcgcggtca aggtcggttg cgagatagat gtggtcggct
     1861 ttttcagcca gttgtttcag ttcagagacg accttctctt taccaggcaa cacttcatag
     1921 tgcgcctccc aattgtgcca cgggtcaacc cccatacggt tgacgagagc gccacgttca
     1981 tcctttttag gctttttagc cgtcttggtg gaggtagagt cggcactctt tttggcagct
     2041 gagccactgg tcggcaaatc gcggatgtga ccgacgctgg atttcaccac gtagtcacta
     2101 cccagatact tgttgatcgt tttggctttt gccggggact caacgatgac aagagcttta
     2161 cccatattca ccttacctaa tttgattctt ccaggaatgc gtcgcacgtt gataaacctt
     2221 ccactggcaa cgagttaccg atatggactc gttctctgcg gatatcaacc ccttgtcgtg
     2281 actctgttca cgaaaattca ggtgactgag ttaacaggtc ttggcctaca ggcgctatag
     2341 cacgacggaa ttttggtcga atgtcaagca aatctattgc cagattgacg aaagcgtcac
     2401 actgtacctg ataaaatccg atacgcaact ttattagcat gcaaaatcca aagttgccag
     2461 taaggcgctc ttgccaccag cgtggtatat ttgtctgcaa gccatgcctg gcgtagacta
     2521 atgtccttat ttaaggagta aataatgaac aaagagacac aacccatcga ccgcgaaacg
     2581 ctgttgaaag aggccaacaa aatcattcgt gagcatgagg atacgctcgc agggattgaa
     2641 gccaccggcg tcacgcagcg taatggcgtg ctggtattta ccggcgacta ctttttagat
     2701 gaacaaggat tgccaacagc caaaagcacc gcggtgttta atatgttcaa acacctggcc
     2761 catgtgcttt cagaaaaata tcatctggtt gattaaagca aaacgcgagg tcttagcctc
     2821 gcgtttgtct tttacatcaa tggcttttga ccccgctgcc accagcgtag caacaatcga
     2881 tcggcgctct ctgccgcgct gccggtgaat cggtcaatga gtcgtttacg ctgcatatag
     2941 cgtacattaa ccacttcacg gccttccatc aggctaagaa taacttcatc actggtgttg
     3001 atttcatcaa ccaggccttt ctctaccgcc tgttgtccgt accagtgttc acctgttgcc
     3061 acctgttcaa tatccagaga cggacgcata cgcttcacaa aatctttaaa caactgatgc
     3121 gtttcgttca actcttcgcg gaatttctcc cgcccttctt cggtattttc acccagcaac
     3181 gtcagagtac gcttatactg cccggcagtg tgcagttcga tatcaatata tttgcttttc
     3241 aggaagcggt taaagttggg catttgcgcc accaccccaa tggaacccac aatagcaaac
     3301 ggtgcggaaa caattttgtc cgccacacag gccatcatgt aaccgccgct ggcagcgact
     3361 ttgtctaccg taacagttaa aggaatgttt ttatcacgca gacgctgcag ctgcgaagcc
     3421 gccaacccgt aaccatgcac catgccacca gggctttcca gacggagcac aacctgatcc
     3481 tgcggtttga atgctgcgag taccgccgtt atctcttcac gtagcgagtt cacttcatgg
     3541 gcgtccatgc tgcctttaaa atccagcacc cagacgcgag gtttactgtc agttgccacc
     3601 tcgcccagtt tggctttcgc ttttgctgct ttcgcttctt gcttgtgctt tttcttctgc
     3661 gctttgtgcc actgtttttg ctgatgtgag cccatcagcg ccgcggccag ttcttctttc
     3721 atctccttat actgttcgct gagattgttg acccgtaact cgccacgctg gcgtttatta
     3781 cgttgagcaa cattgacaat aatggcggca atcgccgcaa tcgctagcac aacggtaacg
     3841 attttcgcca aaaacaaacc atattcagac aacaattcca cgagtcccca ccttggttaa
     3901 acaacgcttt ggctcacagt ttacaacagg catgttaagc cgtctcgcag agatgttatt
     3961 gcagcgaaag tatgacaaag tatccatttt gcggtgttat cgccccaggc tgattgaaat
     4021 gcccttctgt ttcaggcata aagccccaaa gtcataaagt acactggcag cgcgacagac
     4081 aacgtcgttg aggagtagcc atgcattacc agccaaaaca agatttactc aatgatcgca
     4141 ttatcctggt gacgggagcc agcgatggga ttggtcgtga agccgcgatg acgtatgcac
     4201 gctatggtgc gacagtgatt ctgttgggcc gtaatgaaga aaaattacgt caggtagcca
     4261 gccacataaa cgaagaaact gggcgtcagc cacagtggtt tattctcgat ttgctgacct
     4321 gcacgtccga aaattgccaa caactggcac agcgcattgt cgttaattat ccgcgtctgg
     4381 atggtgtttt gcataatgcc ggattgctcg gcgatatttg cccaatgagc gaacaaaatc
     4441 cgcaggtctg gcaggacgtc atgcaggtca acgttaatgc cacctttatg ctcacccagg
     4501 cactgcttcc tttattactc aaatcggacg ccggttcact ggtctttact tcatcaagcg
     4561 ttggacgtca gggacgagct aactggggtg catatgcagc gtcgaaattt gccaccgaag
     4621 ggatgatgca ggtactggcc gatgaatatc agcagcgcct gcgcgtcaac tgcattaacc
     4681 caggcggtac gcgcaccgca atgcgcgcaa gcgccttccc gaccgaagat ccacagaaac
     4741 ttaaaacacc cgctgatatc atgccgctct acctctggct gatgggcgat gacagccgcc
     4801 gtaaaaccgg catgaccttt gacgcccaac cgggccgtaa accaggaatt tcccaatgag
     4861 tgatgaacgc taccaacagc gtcagcagcg agtgaaagaa aaagtagatg ctcgtgtggc
     4921 acaggcccca ggatgaacgc ggtattatca tcgtctttac cggcaatgga aaaggcaaaa
     4981 ccaccgcggc atttggtacg gcaacacgcg cagttggtca cggaaaaaaa gtgggcgtcg
     5041 tgcagtttat taaaggcacc tggcctaatg gcgaacgcaa tctgctggaa ccacatggcg
     5101 ttgagtttca ggtgatggca acgggcttta cctgggatac acaaaaccgc gagtctgata
     5161 ccgccgcctg ccgcgaagtc tggcaacatg caaagcggat gcttgctgat tcctcactgg
     5221 atatggtttt gcttgatgaa ctgacgtata tggtggcgta tgactatttg ccactggaag
     5281 aagtggtgca ggcgttaaat gaacgtccac atcaacagac ggtgattatc acgggtcgag
     5341 gctgtcatcg ggatattctt gaactggcgg atacggtaag tgagctgcgc cccatcaaac
     5401 atgcgtttga tgccggtgtt aaggcgcaaa tagggattga ttattaatct gtaaaaatgc
     5461 cggatatgac gtccggcata ctttgattaa ccgttattac gcccgccaga acgacgaccg
     5521 ccgctcacct gactgtgacg tttcaccgca cgacgaatct gattagcctt catgcgacga
     5581 cggtcttttt ctacagcgac tttagagctg gtttctggcg gaagctccac cagttcgcgc
     5641 agatagttag tctgggcgag gtccagctcc gtccagccac cgcgtggcag accttttggc
     5701 agtgggatat caccgtaacg aacacggatc aggcggctca cctgcacacc aaccgcttcc
     5761 cacagacgac gaacttcacg gttacggcct tcggtcagag tcacgttgta ccactggttg
     5821 atcccttcgc cgccgctgaa cttgatggtt ttaaaagctg ccggaccatc ttccagttgc
     5881 acgccacgac tcaaatcacg cagtttcgcg tcgtcaacct gaccaaatac acgcacggcg
     5941 tattcacgtt caacttcacg gcttggatgc attaaacggt ttgccagttc accatcggtg
     6001 gtgaacagca gcagaccaca ggtgttaacg tccagacgac ctacggcaat ccagcgtgcg
     6061 ccacgcagct ttggcagacg gtcaaacacc gtcggacgcc cttccggatc gttacgggta
     6121 cacaactcac cttccggttt gtaataggcc agaacgcgac aaatttgttc agcggactca
     6181 cgtaccgaaa tcagatgacc atcgatacga attttcagcc caggggtaac ttcaacgcga
     6241 tcgccgagtt tagcaatttt gccatcaaca ctcacacgac cggcttcaat aatagattcg
     6301 atttcacggc gggagccgtg gccagcgcgg gccagcactt tctgtaattt ttcgctcata
     6361 gagcttcctt ttggtgtcgc cttcccaggc gtctgatatg cttttgtgca acagtgagtc
     6421 agaacttaca tctgactcac tgatagtaaa gtctgtttga actcttcagg ccttaagtaa
     6481 acgactcgac gtttatcggc ctggctttga ggcgcaacat actacactat ctttatggtt
     6541 aaagcttgtt tcgattaaca tcagcttaat taccagccac caccgcctcc gccacccgcg
     6601 ccgccgccag caccaccacc ggaataactt cgccccaaca aactggtatt ggccccgcaa
     6661 cttaggtgcg tctgtaatga gaataaaacg ccgttacggg caatctctgg agcgcgaatc
     6721 gccgaagaaa gcgaaggggc aatgcgtaac gcccattccc tccccaaatt tgccgatata
     6781 gcccaaccta gcaacgcgta atccagtttc cgtgtttccc ctttccgccg acgtccactg
     6841 tgtgttgctg cctctttacg cgcaagatag ttaacgatag cttcaccgcg ggcataacgt
     6901 tgctgcccag tttgggtaaa ctttggcaga aaaatatacc ccatgccagt gagataaccg
     6961 gtcaggcaaa cagcgctaaa ataacccgct ggcatatacc agtaaaagaa tacatctccg
     7021 acattgatat agagagaata gaaggccacg cctgaacaaa tcagtggtaa aaacaaagtg
     7081 atgattgcac gattaacgca aggcttgtca tcatcgcggg caaacaaaag ctcaagggaa
     7141 caaaataaaa aaggaataat ccagcagatc ataataaaca tatcgcctac cagactcatt
     7201 cctgcccaac ctacgggact caggttaccg tagagaatga tcgccaatat tgccagtcca
     7261 ccccattgca aaaacgtatc aggccggtaa aacaaagact ttttacgcgg cagataaaat
     7321 ttctgcatcc gtaaaaatgc cctgcgtaag actctgttgc gtctccccgt aagaacgacc
     7381 ttatcgcctt tacgaaataa cgcttccagt aataactgct ctgcgggcag taatacctca
     7441 ttatttctgg tcgcgccttc attaacacaa atcaggattt gctggttctt atcatactga
     7501 tcttccagat gaatttttcc cttcacgatc agatttacga tatcggcaca gaaaccttta
     7561 tcgtcgtaaa ccagcttcgc atcgagacgt aacatgccgg gcgtgtaatc tggcggaatg
     7621 acatcggttt caatcacatc taccggcgta aattgcggcc tgcgcttcca gagatataac
     7681 catccacaaa ccaggaatag acacggaaac caaattaaca acgatgacgt ggagggtaaa
     7741 agaagatggc tgaaaatatt cgtcgtttgt ggagccgggg cattgctaag taacgcgtga
     7801 ggccagcggt agagtaccgt gaaatcttca cgataaaacg gatcgcggga ttctattctt
     7861 ccgtcctcaa ggatgcggcc atttcgatat gtgtcgccct cttctccagt aaagagatcg
     7921 atttcgctaa atggattacc cgcgatatca gggaacttga gtgaaaatcg ggtcttatag
     7981 atttcaaacg gccagtggtt accagtcacg ttccagatta acagatctga atctccctca
     8041 cgcaggaaag catttttaac ctgataatga atttgataat gatagtcgcc ttgaggcaag
     8101 aaacgttgtt tatcgcccac gacaatactc attaacccga tatgccagtc aagatgccag
     8161 ggttcaggaa taccatcacg agtgacgcca acaatatgat agttcagcag tgcagcgtcc
     8221 ccatcaggct gcatccagag tcgtggtaaa tcacgataaa atccgtgctt aatctgctga
     8281 tttcgcgcct gaaaatgaat attttctttg atgtcgagca ccccatccgg ttgcagagtg
     8341 gcttcaacat caaagagcga gatcccttct tcataagaag ggatctccgg gttttctgca
     8401 taacttagag atgatacaaa cagccctgct atcaacagca aaatgcagcg aaacttccct
     8461 gccatagtct tccatccgtg gaacgcaatt tgtcataaga aaggcttcac atcacccacg
     8521 ccttcacgca ccacgaccgg cgtatcatcg gtaagatcaa tgaccgttgt cggtttctgt
     8581 ccgagatagc cgccgtggat aatcaaatcc acctgttttt ccagacggtc tttaatttct
     8641 tctggatcgg attcggtaaa ttcgctgcct ggcagcatta gcgaagtgga aagcatcggt
     8701 tcgcccagtg cttcaagcaa cgcctgcgca atcgggttag aaggcacacg cataccgatg
     8761 gttttgcgtt tttcctgcaa cagacggcgt ggcacctcct tcgtcccctt caggatgaag
     8821 gtatagttgc ccggcgtgtt gtttttcatt aaacgaaacg caacgttatc cacgaatgaa
     8881 taggtcgaaa gttcagaaag atcgcgacac atcagggtaa agttgtgacc gtccggcagc
     8941 tggcgaatac gacaaatacg ctccatcgcg tttttatctt caattttaca gccgagcgca
     9001 tagccggaat cagttggata aacaatcacc ccgcctttac gcacgatctc caccgcctgg
     9061 ttgatcagac gttgctgcgg gttatcagga tgaatataaa aaaactggct cataattccc
     9121 tctctgtggt gttctgcggc tgttcccata gctgccagac gccttcaacg cctgcgggca
     9181 gccagagttt acgacccagt tcgatccacg ggcatggctg atgaaaatca gatccttgtg
     9241 atgcccataa atgatgctga cgcgcaagtg tcgtcaactg ggtgcgttca ttgggcgatt
     9301 gctggcactg cgcgacttcc atcgcgtcgc cgtggtgttc ggcaaaatgc gcaaccaatc
     9361 ttttcagcca tttagcacta agattgtacc gcccaggatg agctagcacc gccttaccgc
     9421 cagaatgatg aatgacatca atagcttgtt ctattgtaca ccactgtggc ggaacgtatc
     9481 cggttttccc gcgcgccaga tactttttaa agacatctgc cattgaactg gctttgccgc
     9541 actcaacaag gaaacgcgca aaatgaccgc gcgtcactgc gccgccctgc gccagtcgtt
     9601 gtgcgccttc cagcgcccca ggtatttgcg ctttttcaag tctttcggca atttgctgcg
     9661 cccgctgatt gcgccgttct gtctgctgcg cgaggaactc acacattagc ggatgagtaa
     9721 tatcaatatt cagcccgaca atatgaattt catgattttc ccagaccgtg gaaatttcca
     9781 cgccgggaat aagattcagc gccagtcccg aacgtgaaat ttcctctctt gctggcgcga
     9841 ttgcagctgt ggtgtcatgg tcggtgatcg ccagggtgcc gatgcgcatc tcgactgcac
     9901 ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt
     9961 cgtaaatcac tgcgtaattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt
    10021 tgcgccgaca tcataacggt tctggcaata ttctgaaatc agctgttgac aattaatcat
    10081 cgaactagct aactagtacg caagttcacg taaaaagggt atcgacaatg aaagcaattt
    10141 tcgtactgaa agcttggtgg cgcacttcct gaaacgggca gtgtattcac catgcgtaaa
    10201 gcaatcagat acccagcccg cctaatgagc gggctttttt ttgaacaaaa ttagagaata
    10261 acaatgcaaa cacaaaaacc gactctcgaa ctgctaacct gcgaaggcgc ttatcgcgac
    10321 aacccgactg cgctttttca ccagttgtgt ggggatcgtc cggcaacgct gctgctggaa
    10381 tccgcagata tcgacagcaa agatgattta aaaagcctgc tactggtaga cagtgcgctg
    10441 cgcattacag cattaggtga cactgtcaca attcaggcac tttccggcaa cggcgaagcc
    10501 ctgctgacac tactggataa cgccctgcct gcgggtgtgg aaaatgaaca atcaccaaac
    10561 tgccgtgtgc tgcgcttccc ccctgtcagt ccactgctgg atgaagacgc tcgcttatgc
    10621 tccctttcgg tttttgacgc tttccgttta ttgcagaatc tgttgaatgt accgaaggaa
    10681 gaacgagaag caatgttctt cggcggcctg ttctcttatg accttgtggc gggatttgaa
    10741 gatttaccgc aactgtcagc ggaaaataac tgccctgatt tctgttttta tctcgctgaa
    10801 acgctgatgg tgattgacca tcagaaaaaa aacacccgca ttcaggccag cctgtttgct
    10861 ccgaatgaag aagaaaaaca acgtctcact gctcgcctga acgatcttcg ccagcagctg
    10921 accgaagccg cgccgccgct gccggtggtt tccgtgccgc atatgcgttg tgaatgtaac
    10981 cagagcgatg aagagttcgg tggcgtggtg cgtttgttgc aaaaagcgat tcgcaccgga
    11041 gaaattttcc aggtggtgcc gtctcgccgt ttctctctgc cctgcccgtc accgctggcg
    11101 gcctattacg tgctgaaaaa gagtaatccc agcccgtaca tgttttttat gcaggataat
    11161 gatttcaccc tgtttggcgc gtcgccggaa agttcgctca agtatgacgc caccagccgc
    11221 cagattgaga tctacccgat tgccggaaca cgcccgcgcg gtcgtcgcgc cgatggttca
    11281 ctggacagag acctcgacag ccgcatcgaa ctggaaatgc gtaccgatca taaagagctt
    11341 tctgaacatc tgatgctggt ggatctcgcc cgtaatgatc tggcacgcat ttgcaccccc
    11401 ggcagccgct acgtcgccga tcttaccaaa gttgaccgtt actcttacgt gatgcacctg
    11461 gtctcccgcg tggtcggtga gctgcgccac gatctcgacg ccctgcacgc ttaccgcgcc
    11521 tgtatgaata tggggacgtt aagcggtgcg ccgaaagtac gcgccatgca gttaattgcc
    11581 gaggcggaag gtcgtcgccg cggcagctac ggcggcgcgg taggttattt caccgcgcat
    11641 ggcgatctcg acacctgcat tgtgatccgc tcggcgctgg tggaaaacgg tatcgccacc
    11701 gtgcaagccg gtgctggcat agtccttgat tctgttccgc agtcggaagc cgacgaaacc
    11761 cgtaataaag cccgcgctgt actgcgcgct attgccaccg cgcatcatgc acaggagact
    11821 ttctgatggc tgacattctg ctgctcgata atatcgactc ttttacgtac aacctggcag
    11881 atcagttgcg cagcaatggg cataatgtgg tgatttaccg caaccatatt ccggcgcaga
    11941 ccttaattga acgcctggcg acgatgagca atccggtgct gatgctttct cctggccccg
    12001 gtgtgccgag cgaagccggt tgtatgccgg aactcctcac ccgcttgcgt ggcaagctgc
    12061 ccattattgg catttgcctc ggacatcagg cgattgtcga agcttacggg ggctatgtcg
    12121 gtcaggcggg cgaaattctc cacggtaaag cctccagcat tgaacacgac ggtcaggcga
    12181 tgtttgccgg attaacaaac ccgctgccag tggcgcgtta tcactcgctg gttggcagta
    12241 acattccggc cggtttaacc atcaacgccc attttaatgg catggtgatg gcagtacgtc
    12301 acgatgcgga tcgcgtttgt ggattccagt tccatccgga atccattctt actacccagg
    12361 gcgctcgcct gctggaacaa acgctggcct gggcgcagcg gaaactagag ccaaccaaca
    12421 cgctgcaacc gattctggaa aaactgtatc aggcgcagac gcttagccag caagaaagcc
    12481 accagctgtt ttcagcagtg gtacgtggcg aactgaaacc ggaacaactg gcggcggcgc
    12541 tggtgagcat gaaaattcgc ggcgagcacc cgaacgagat cgccggagca gcaaccgcgt
    12601 tactggaaaa cgccgcgccg ttcccgcgcc cggattatct gtttgccgat atcgtcggca
    12661 ccggtggtga cggcagtaac agcatcaata tttccaccgc cagtgcgttt gtcgccgcgg
    12721 cctgtgggct gaaagtggcg aaacacggca accgtagcgt ctccagtaaa tctggttcgt
    12781 ccgatctgct ggcggcgttc ggtattaatc ttgatatgaa tgccgataaa tcgcgccaag
    12841 cgctggatga gttaggcgta tgtttcctct ttgcgccgaa atatcacacc ggatttcgcc
    12901 atgcgatgcc ggttcgccag caactgaaaa cccgcactct gttcaacgtg ctgggaccat
    12961 tgattaaccc ggcgcatccg ccgctggcgt taattggtgt ttatagtccg gaactggtgc
    13021 tgccgattgc cgaaaccttg cgcgtgctgg ggtatcaacg cgcggcggta gtgcacagcg
    13081 gcggtatgga tgaagtttca ttacacgcgc cgacaatcgt tgccgaactg catgacggtg
    13141 aaattaagag ctatcagcta actgcagaag attttggcct gacaccctac caccaggagc
    13201 aactggcagg cggaacaccg gaagaaaacc gtgacatttt aacacgcttg ttacaaggta
    13261 aaggcgacgc cgctcacgaa gcagccgtcg cggcgaatgt cgccatgtta atgcgcctgc
    13321 atggccatga agatctgcaa gccaatgcgc aaaccgttct tgaggtactg cgcagtggtt
    13381 ccgcttacga cagagttacc gcactggcgg cacgagggta aatgatgcaa accgttttag
    13441 cgaaaatcgt cgcagacaag gcgatttggg tagaaacccg caaacagcag caaccgctgg
    13501 ccagttttca gaatgaggtt cagccgagca cgcgacattt ttatgatgcg ctacagggtg
    13561 cacgcacggc gtttattctg gagtgcaaga aagcgtcgcc gtcaaaaggc gtgatccgtg
    13621 atgatttcaa tccggcacgc attgccgcca tttataaaca ttacgcttcg gcaatttcgg
    13681 tgctgactga tgagaaatat tttcagggga gctttgattt cctccccatc gtcagccaaa
    13741 tcgccccgca gccgatttta tgtaaagact tcattatcga cccttaccag atctatctgg
    13801 cgcgctatta ccaggccgat gcctgcttat taatgctttc agtactggat gacgaccaat
    13861 atcgccagct tgcagccgtc gctcacagtc tggagatggg ggggctgacc gaagtcagta
    13921 atgaagagga actggagcgc gccattgcat taggggcaaa ggtcgttggc atcaacaacc
    13981 gcgatctgcg tgatttgtcg attgatctca accgtacccg cgagcttgcg ccgaaactgg
    14041 ggcacaacgt aacggtaatc agcgaatccg gcatcaatac ttacgctcag gtgcgcgagt
    14101 taagccactt cgctaacggt tttctgattg gttcggcgtt gatggcccat gacgatttgc
    14161 acgccgccgt gcgccgggtg ttgctgggtg agaataaagt atgtggcctg acgcgtgggc
    14221 aagatgctaa agcagcttat gacgcgggcg cgatttacgg tgggttgatt tttgtcgcga
    14281 catcaccgcg ttgcgtcaac gttgaacagg cgcaggaagt gatggctgcg gcaccgttgc
    14341 agtatgttgg cgtgttccgc aatcacgata ttgccgatgt ggtggacaaa gctaaggtgt
    14401 tatcgctggc ggcagtgcaa ctgcatggta atgaagatca gctgtatatc gatacgctgc
    14461 gtgaagctct gccaacacat gttgccatct ggaaagcatt aagcgtcggt gaaaccctgc
    14521 ccgcccgcga gtttcagcac gttgataaat atgttttaga caacggccag ggtggaagcg
    14581 ggcaacgttt tgactggtca ctattaaatg gtcaatcgct tggcaacgtt ctgctggcgg
    14641 ggggcttagg cgcagataac tgcgtggaag cggcacaaac cggctgcgcc ggacttgatt
    14701 ttaattctgc tgtagagtcg caaccgggca tcaaagacgc acgtcttttg gcctcggttt
    14761 tccagacgct gcgcgcatat taaggaaagg aacaatgaca acattactta acccctattt
    14821 tggtgagttt ggcggcatgt acgtgccaca aatcctgatg cctgctctgc gccagctgga
    14881 agaagctttt gtcagcgcgc aaaaagatcc tgaatttcag gctcagttca acgacctgct
    14941 gaaaaactat gccgggcgtc caaccgcgct gaccaaatgc cagaacatta cggctgggac
    15001 gaacaccacg ctgtatctga agcgcgaaga tttgctgcac ggcggcgcgc ataaaactaa
    15061 ccaggtgctc ggtcaggctt tactggcgaa gcggatgggt aaaaccgaaa tcatcgccga
    15121 aaccggtgcc ggtcagcatg gcgtggcgtc ggcccttgcc agcgccctgc tcggcctgaa
    15181 atgccgtatt tatatgggtg ccaaagacgt tgaacgccag tcgcctaacg tttttcgtat
    15241 gcgcttaatg ggtgcggaag tgatcccggt acatagcggt tccgcgacgc tgaaagatgc
    15301 ctgtaacgag gcgctgcgcg actggtccgg cagttatgaa accgcgcact atatgctggg
    15361 caccgcagct ggcccacatc cttatccgac cattgtgtgt gagtttcagc ggatgattgg
    15421 cgaagaaacg aaagcgcaga ttctggaaag agaaggtcgc ctgccggatg ccgttatcgc
    15481 ctgtgttggc ggcggttcga atgccatcgg catgtttgca gatttcatca acgaaaccaa
    15541 cgtcggcctg attggtgtgg agccgggtgg tcacggtatc gaaactggcg agcacggcgc
    15601 accgctaaaa catggtcgcg tgggcattta tttcggtatg aaagcgccga tgatgcaaac
    15661 cgaagacggg cagattgaag aatcttactc catctccgcc ggactggatt tcccgtctgt
    15721 cggcccacaa cacgcgtatc ttaacagcac tggacgcgct gattacgtgt ctattaccga
    15781 tgatgaagcc cttgaagcct tcaaaacgct gtgcctgcac gaagggatta tcccggcgct
    15841 ggagtcttcc cacgccctgg cccatgcgct gaaaatgatg cgcgaaaccc cggaaaaaga
    15901 gcagctactg gtggttaacc tttccggtcg cggcgataaa gacatcttca ccgttcacga
    15961 tattttgaaa gcacgagggg aaatctgatg gaacgctacg aatctctgtt tgcccagttg
    16021 aaggagcgca aagaaggcgc attcgttcct ttcgtcacgc tcagggatcc gggcattgag
    16081 cagtcattga aaattatcga tacgctaatt gaagccggtg ctgacgcgct ggagttaggt
    16141 atccccttct ccgacccact ggcggatggc ccgaccattc aaaacgccac tctgcgcgcc
    16201 tttgcggcag gtgtgactcc ggcacaatgt tttgaaatgc tggcactgat tcgccagaaa
    16261 cacccgacca ttcccattgg cctgttgatg tatgcca
//
LOCUS       DAJTNN010000089        15964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTNN010000089 DAJTNN010000000
VERSION     DAJTNN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.089"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            10..1245
                     /locus_tag="OO150_002472"
     CDS             10..1245
                     /locus_tag="OO150_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5516577.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISFGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDSYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            1297..2232
                     /gene="ttcA"
                     /locus_tag="OO150_002473"
     CDS             1297..2232
                     /gene="ttcA"
                     /locus_tag="OO150_002473"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5516578.1"
                     /translation="MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGIPHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2361..3734)
                     /gene="dbpA"
                     /locus_tag="OO150_002474"
     CDS             complement(2361..3734)
                     /gene="dbpA"
                     /locus_tag="OO150_002474"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5516579.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4180..5163)
                     /gene="zntB"
                     /locus_tag="OO150_002475"
     CDS             complement(4180..5163)
                     /gene="zntB"
                     /locus_tag="OO150_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5516580.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5418..6650
                     /gene="dgcM"
                     /locus_tag="OO150_002476"
     CDS             5418..6650
                     /gene="dgcM"
                     /locus_tag="OO150_002476"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR5516581.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6671..7234)
                     /gene="smrA"
                     /locus_tag="OO150_002477"
     CDS             complement(6671..7234)
                     /gene="smrA"
                     /locus_tag="OO150_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR5516582.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7564..8472)
                     /locus_tag="OO150_002478"
     CDS             complement(7564..8472)
                     /locus_tag="OO150_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5516583.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            8648..9948
                     /gene="abgA"
                     /locus_tag="OO150_002479"
                     /pseudo
     CDS             8648..9948
                     /gene="abgA"
                     /locus_tag="OO150_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
     gene            9948..11393
                     /gene="abgB"
                     /locus_tag="OO150_002480"
     CDS             9948..11393
                     /gene="abgB"
                     /locus_tag="OO150_002480"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR5516584.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANFPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQIQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSSLK"
     gene            11430..12956
                     /gene="abgT"
                     /locus_tag="OO150_002481"
                     /pseudo
     CDS             11430..12956
                     /gene="abgT"
                     /locus_tag="OO150_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            12967..13482
                     /gene="ogt"
                     /locus_tag="OO150_002482"
     CDS             12967..13482
                     /gene="ogt"
                     /locus_tag="OO150_002482"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5516585.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREIWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13677..14429
                     /gene="fnr"
                     /locus_tag="OO150_002483"
     CDS             13677..14429
                     /gene="fnr"
                     /locus_tag="OO150_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5516586.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14581..15531
                     /gene="uspE"
                     /locus_tag="OO150_002484"
     CDS             14581..15531
                     /gene="uspE"
                     /locus_tag="OO150_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5516587.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFEPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(15581..15839)
                     /locus_tag="OO150_002485"
                     /pseudo
     CDS             complement(15581..15839)
                     /locus_tag="OO150_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT         4137 a         3951 c         3913 g         3963 t
ORIGIN      
        1 actcatgata tgtctaaatt accaacaggt gtcgagattc gaggtaaata cattcgcatc
       61 tggttcatgt ttcgaggaaa acgatgtcgg gaaacattga aaggctggga gattactaac
      121 agtaacatta aaaaggccgg gaatttaaga gcgttgatag ttcatgaaat cagtttcggt
      181 gaatttgagt atttaagacg ttttccccag tccagcactg gggcaaaaat ggtgacaacg
      241 agggtcataa aaacattcgg ggagctttgt gatatctgga caaaaattaa agagacagag
      301 ttaacaacaa acacaatgaa gaaaacgaaa tcacaattaa aaacactcag gataataatt
      361 tgtgagagta ccccgatatc gcatattcgt tatagcgata tcttaaacta ccggaatgaa
      421 ctgctgcatg gagaaacgct ttacctagat aatccaagat ccaacaaaaa aggaagaacc
      481 gtgcgcacag ttgatagcta tatcgccctg ctctgttcgt tgttacgttt tgcgtatcag
      541 tcgggattta tatcaaccaa accatttgaa ggagtaaaaa aattacagcg aaacagaata
      601 aaacctgacc cgttatctaa aacagaattc aatgcattaa tggaaagtga aaaaggacag
      661 agccaaaact tgtggaaatt tgccgtttac tccgggcttc gtcacgggga actggcagct
      721 ctggcgtggg aggatgtgga tttcgagaag ggaattgtga atgtcagaag aaacctgacg
      781 atacttgata tgttcggtcc cccaaaaaca aatgccggga tccggacggt aacattactg
      841 cagccggctc ttgaagcact gaaggagcaa tacaaactga ccgggcatca tcgcaaaagc
      901 gaaatcactt tttatcatcg ggagtacggc agaactgaaa agcaaaaact gcattttgtt
      961 ttcatgccca gggtgtgtaa cgaaaaacag aaaccttatt actcggtaag cagtttgggt
     1021 gcaagatgga atgcagcagt aaaacgtgct ggtattcgcc gccgtaatcc gtaccatacg
     1081 cgacatactt ttgcctgctg gctgttgacg gcaggagcga acccggcatt tatagccagc
     1141 cagatggggc atgaaactgc gcaaatggtg tatgaaattt acggtatgtg gattgatgac
     1201 atgaacgacg aacagatagc catgttgaat gcgcggttat cgtagttgca aagtttgccc
     1261 ccaatttgcc ccatttagta ccagagaact gaaataatgc aagaaaatca acaaattaca
     1321 aagaaagaac aatacagcct gaacaaatta caaaaacgtc tacgtcgtaa cgtgggcgaa
     1381 gccattgctg acttcaatat gattgaagaa ggcgatcgca tcatggtttg cctctcaggg
     1441 ggtaaagaca gctataccat gctggagatt ctgcgcaatt tgcagcaaag cgcgccaatc
     1501 aatttttcgc tggtggctgt taacctcgat caaaagcaac cgggcttccc agaacacgtt
     1561 ctgcccgtgt atcttgaaaa gctgggcgtt gagtacaaga ttgtcgaaga gaacacttac
     1621 ggtatcgtga aagagaagat tccagagggc aaaaccactt gctcactgtg ttctcgcctt
     1681 cgtcgcggta tcctttatcg taccgcaacg gaactggggg cgacgaagat cgcgctgggt
     1741 caccatcgtg acgatatcct gcagacgttg ttcttaaata tgttctacgg cggcaagatg
     1801 aaaggtatgc ctccgaaact gatgagcgat gatggcaaac atatcgttat tcgtccgctg
     1861 gcctactgcc gcgagaaaga tattcagcga tttgccgatg caaaagcgtt cccgattatt
     1921 ccgtgcaacc tgtgcggttc acagcctaac ctgcaacgtc aggtgattgc tgacatgttg
     1981 cgtgactggg ataaacgtta tccagggcgt atcgagacga tgttcagcgc gatgcagaat
     2041 gtggtgccgt cgcatctgtg cgataccaac ctgttcgatt tcaaaggcat cccccacggt
     2101 tctgaagtgg ttaacggggg tgatctggcg tttgatcgcg aagagatccc actacaaccg
     2161 gcgggctggc agccagaaga agatgaaaat cagttggatg agttacggct gaatgtggtt
     2221 gaagtgaaat aaccaggata gcgcccgatg cgcaagcgta tcgggctact cttatggagg
     2281 ccggataaga cgcggccaac gtcgcatccg gcaatgccga agaagatgtt tactcttgca
     2341 cccggcaatt caacatttca ttattttaat aaccgcaccc ggcacgtttt tcctttaatc
     2401 ttcccgccct gtaactgttt ccatgcttta tgagcaacag cctgacggac cgcgacatag
     2461 acatgcgccg gatgcacggc gattttgcca atatctgcgc catcaagccc gatatctcct
     2521 gtcagtgcac ctaatacatc acccgggcgc attttggctt ttttcccgcc atcgatacac
     2581 aacgttgcca tttctgcttc cagcggcaca atggaactat tagctggcgg cgtttgccag
     2641 ttaagtttta tctgcaacat gtcagaaatg atattggccc gctgtgcttc ttccggagca
     2701 cagaaactga tcgccagacc gctatttcct gcacgagctg tacggccgat gcgatgtaca
     2761 tgaacttcag ggtcccacgc cagctcaaag ttcaccacca gctcaagcga tttaatatcc
     2821 agaccacgcg cagcaacatc agtcgcaacc agtacacggg cgctaccgtt agcaaaacgt
     2881 accagggtct gatcgcgatc gcgttgctcc aaatcgccgt gtaatgacaa tgcactttgc
     2941 cctacttcat tcagcgcgtc gcagacagcc tggcaatctt ttttggtatt gcaaaacacc
     3001 acgcaagagg atggctgatg caagcttaat aaccgttgca acagaggaat tttgcctttg
     3061 ctggatgtct cataaaattg ttgttcaatg ggtggcaaag catctgttga gtcaatttca
     3121 atcgccaaag gatcgcgttg cactcgtccg ctgattacgg cgatggcttc cggccaggtt
     3181 gccgaaaaca gaagcgtctg tcgagatgca ggcgcaaaac ggatgacatc atcaatggca
     3241 tcgctaaatc ccatatccag catgcggtcg gcctcatcca tcaccaaagt attcagcgca
     3301 tccagtgata ccgtgccttt ttgcaagtga tccagcaaac gacccggcgt tgccacgata
     3361 atatgcggcg catgttgcaa cgaatcacgc tgcataccga acggttgacc accgcacaac
     3421 gtcaaaattt tggtatttgg cagaaaacgc gccagccgac gcaattcacc tgccacctga
     3481 tccgccagtt cacgcgtagg acacagcact aaagcctggg tttgaaatag cgacgcatca
     3541 atttgctgta acaagccgag gccaaaagcc gccgttttgc cgctgccggt tttcgcctgc
     3601 acgcgaacat cttttccggc aaggatcgcc ggaagcgcgg cggcctgcac cggcgtcata
     3661 gttaaataac ccaactcatt aaggttcgtg agttgggcgg gaggcaaaac attcagggta
     3721 gaaaaagcgg tcacaatcta ttctcgtggt catcgacgca aagttagcag gcgcgtatcc
     3781 tcgcagatct acgcgggtgg tgggtcaggc atgggttgcg ggcgagggat cggatcgggc
     3841 actggaacag gatcgccagg aatcggttca gggacaggaa tttgcaaata aataagtgtc
     3901 gtcatatttc cctccggtca ttgggtggac tcttaaaggg tagacgctga taaataacag
     3961 gcaaaaaaaa gccgactcat caaagtcggc gtcgtacgaa tcaattgtgc tatgcagtaa
     4021 ttcaaaaaag gaagtaagac aatatggagc gcaacgccca tcgcttgacg ttgcattcac
     4081 ctgcaaggga gatattgccc tgaatgggta gagagtttat tgacttcgct caaactttgc
     4141 ggcgtttttg tatacagaca gccggaaaaa ttgcttttgt tacaaccatt tactacgatg
     4201 caaccataaa gcaacaccac caataagaac aactaacaga atacaaaaaa ttgaaaatcc
     4261 gaattgccac ccgccgccag ggatcccacc aaggttgacg ccaaataacc ctgtcagaaa
     4321 ggtactgggt aaaaagacca ttgccatcaa tgacattgta taggtacgac gagctaaatt
     4381 ttcctgcatc acctgagcga tttcatccgc catcacgcca gtccgtgcta tacaggcgtc
     4441 gatttcgtca agaccgcgcc caaggcgatc ggcaatatcc tgcatccgac ggcgttggtc
     4501 atcgctcatc cacggcaaac gttcactggc aagacgagca taaacatcac gttgcggtgc
     4561 catatagcga cgcatcacga ttaattgttt gcgcagcaga gccaggaatc cacgcggtgg
     4621 aatttgctga tcaaggagat tatcttcaag gtcgataatt ttatcgtgca gctgctcgat
     4681 aaattcgctg gaatgatcgg tcaacgcatc gcacacatcc accagccacc ctccgcaatc
     4741 ggtcggaccc gtgccctctt ccagatcgct caccacatcg tccagcgcca gcactttgcg
     4801 ttgtcgggtc gaaacaatta accgcccgtc catatataca cgcatggcga ccagttgatc
     4861 ggggcgttca tcggtgctgc cgtttataca gcgcaatgta atcagcgtgc cttcaccgag
     4921 acggctgact cggggccgtg tgctctcgcc tgccagcgca tcacgtacgt tattgggaag
     4981 cagcggtgtt gtcgccagcc attgggcgct atcatggtgt acataattaa ggtggagcca
     5041 gcagggatgc gcttcatcaa tcacatctgt attttccagc ggtttaacgc cgcctctacc
     5101 atccagcatc caggcaaata ctgcatccgg gacattaacg tccgatccct taatcgcttc
     5161 cacagtgcct ccatcatcaa cgcattattt tgtagtctag ccttctggcc ctgttacgca
     5221 acatctcatc accccattac cctgaaatga ttaataaaat tctgtctaaa ttgaatacaa
     5281 aaagcaaaat gcttttccgt atacaaaccg tgtgaagtgt taaatagcgt ctatcattat
     5341 cagaattatc tgatcatatg acgtggcttt tttgcgatcg gatagcaaca aaaattgata
     5401 aaaataacgg gatctcaatg attacgcaca acttcaatac cctggactta ctcaccagtc
     5461 ctgtctggat tgtttcgccc tttgaggaac agttaattta tgccaatagc gcggcgcgac
     5521 tgttgatgca agacctcacg tttagtcagc tacgaaccgg accctattcc gtctcctcac
     5581 aaaaagaact gccgaaatac ctctccgatc tgcaaaacca acacgatatt atcgaaatcc
     5641 tcactgttca gcgtaaagaa gaggaaacag cattaagctg tcggcttgtt ttgcgagagc
     5701 tgacagaaac agaaccggtg attattttcg aaggtatcga agcgccggca acgctgggtt
     5761 taaaagccag tcgctcggca aattatcagc gcaaaaaaca aggtttttat gcgcgctttt
     5821 ttctgactaa ctctgcacca atgttgttga ttgacccctc acgagatggc caaatcgtcg
     5881 atgctaacct cgccgcgctc aatttctacg gttataacca tgaaacgatg tgccagaaac
     5941 atacctggga aattaatatg ctcgggcgtc gcgtcatgcc tatcatgcat gaaatttcgc
     6001 atttacccgg cggtcataaa cctttgaatt ttgttcataa actggcggat ggatctactc
     6061 gtcatgtgca gacctatgcc gggccgattg aaatttatgg cgacaagctc atgttatgta
     6121 ttgtgcatga tattactgag caaaaacggc tggaggagca gctggaacat gctgctcacc
     6181 atgacgcgat gaccggatta ctgaatcggc gacagtttta tcacattacg gaaccaggcc
     6241 aaatgcagca cctcgccatc gcccaggatt acagcttgtt gctcatcgac accgatcgtt
     6301 ttaaacacat taacgatctc tatgggcatt ctaaaggtga tgaggtgtta tgcgccctcg
     6361 cccgcaccct cgaaagttgc gctcgcaaag gcgatttggt gtttcgttgg ggaggcgaag
     6421 agtttgtctt attgctacca agaaccccac tggataccgc gctttcgctg gctgaaacta
     6481 tccgtgtaag cgtggccaaa gtgagtattt cgggcttacc acgttttacc gtcagcattg
     6541 gtgtggcgca tcacgaagga aatgaaagca tcgatgaact gtttaaacgc gttgatgatg
     6601 ctttgtatcg ggcgaaaaat gatggacgca accgcgtgct ggcggcataa gccgcggatg
     6661 cgtctcgaga tcaacggctg cgcttagcgt ggcgctccca gttttcttgc ttcgcctgcg
     6721 ccgttttacg tagtgcgacg taacacgccc cgctgccgcc atgatgcggt agcgcggtgc
     6781 aatatgcctg aacatcatca aattcggtca gccagcgcgc cacatagctg cggacaatat
     6841 tggcatgcga tttatcttcc cgccctttac catgaataat cagcacatta cgcaaaccat
     6901 ccgccagggc ttggtgaatg aaactgaaca ccattttgcg gcactcttcc accggctggc
     6961 gcaaaagatt caggctcgcc tgttgcggat atttaccact gcgcagctta tccagcaccc
     7021 catgttgcaa cccttcccgc cgaaactcca gcggctgact tagcgggatg atgtcgagaa
     7081 atccggtggt gaggaaatta tcaagctgca gcgtgtcgat acgctgcggc gcacgttggt
     7141 tacgcgttgg atgccagtgg acatctgtag cacgtttcag cggctggaca tcttccatgg
     7201 cgtcaagaaa cagcgatttg tcgtcaaggt tcatgttaca tcctcccgca attaagagcg
     7261 cgatatgata accagaccgg gtcggtccaa caacgtatta ccaaaaattc cagtaataag
     7321 ttccaaatat tgccgatatt ttaagcaaaa tgcttatgca tgattattca ttcacgatat
     7381 taataatgta acttatattt tcgtgaaatc tgtcactgaa gaaaattggc aactaaaggt
     7441 taaaaccgtt ataacacagt caccggcgca gaggagacaa tgccggactt aagacgcgga
     7501 tgcactgctg tgtgtactgt agagtctggc ggatgtcgac agactctatt tttttatgca
     7561 gttttaactt tgcagatagc cgcattctcg ccggaattgc gtgattaatg acggggtcag
     7621 tggtgtctgg cgactatcac gccgctgaat caaataatag gccgctttcg gtaaaatttc
     7681 gctaaccggc aacatcacca gcccctgtcc gtgcaaggga tcgcagccca tttcttcagg
     7741 cagtatgctg agaaaatcgc tttttgccac cagactgata caggctgaga acgtctcgca
     7801 gactacaccg acctgtggcg tttgcgcctg atcgtcaagc aattcactca actgtttgta
     7861 gtaactgccg tgtggcgtcg gcattgtcca gctgtaatcc agtaactgtt tgatcgaacg
     7921 ggcaccaatg gcggggtgtc ccgggcggca aaagatcgcg aattgctttt ccagtaattt
     7981 ctcaaaagta aattcgtggt cgtacggtcc ctgataatag gtattgatgg tgaaatccaa
     8041 ttctccctga cgcaattcat taatcatcga caccagttgc ccttccataa tgcgtacttt
     8101 tacctgcgga tgctgctgat gaaaacgaga tatgacagct ggcatcagac tgcgggaaat
     8161 actggccccc atgccgatat taatctgccc tgccagttgc ccttgtcgtt ggcgaatatc
     8221 ctcttgggct gcgcgcagct cttcaagaat aagactggcg tgctgataaa aactttcacc
     8281 ggcatcagtt aacgtcacgc ctttactacg gcgaaaaaag agttgcgccg ctaatccttc
     8341 ttccaactcc tgaatagatt tactcagtgc cggttgcgac atattcaaca ttcggctcgc
     8401 tccgcgaatg ctgccctgac gagccacttc aacaaaagca cgaatttgat gaatttttac
     8461 ctgaaaagcc atgacgccac cgataaccgt tatttatcag accaaagaaa ctggcatcta
     8521 ctttaatgca gatgattgtg tcagggtaat ttatgaacgg ttaaaactgt gaaaaatcag
     8581 ttagtgataa gtaaaaacta tcgctacgtg aaccgggtca cactttttac tgatgacggg
     8641 aaaggttatg gagtctttga atcaatttgt taattcgctt gccccaaaat tatcgcactg
     8701 gcgacgtgat tttcatcact atgcagagtc tggctgggtg gaattccgca ctgccaccct
     8761 tgttgcggaa gaattgcagc agctcggcta ttcactggcg ctgggccgcg aagtagttaa
     8821 tgaaagtagc cggatgggac tacctgatga actcactcta caacgcgaat tcgagcgcgc
     8881 tcgtcaacag ggtgcgctag cacaatggat tgcggttttt gaaggtggtt tcactggtat
     8941 cgtcgccacc ctggataccg gtcgccccgg tccggtgatg gctttccgtg tcgatatgga
     9001 cgcgctggat ctcagtgaag agcaggatgt cagccatcgc ccctaccgcg acggttttgc
     9061 gtcatgtaac gccggaatga tgcatgcctg tggtcatgat ggacataccg ccattgggct
     9121 tgggctagcg cataccctta aacaattcga gtccggacta catggcgtca tcaaactgat
     9181 ttttcagcct gcagaggaag gtacgcgtgg cgcgcgggcg atggtcgatg caggtgtcgt
     9241 agatgatgtt gattatttta ctgccgtgca cattggcact ggcgtacctg cgggcaccgt
     9301 ggtgtgcggc agtgataatt ttatggcaac caccaaattt gacgcgcact tcaccggtac
     9361 cgccgctcac gcaggcgcaa aaccagaaga cggtcacaat gccttgttgg cggcagcaca
     9421 agccactctt gcactgcatg caatcgcccc ggagcttcca gagtaaacgt gggcgttatg
     9481 caggcaggaa gcggtcgtaa cgttgttcct gcctcggcgt tgctgaaagt ggaaacacgc
     9541 ggggccagcg acgtcattaa tcaatatgtt tttgaccgtg cacaacaagc gattcagggc
     9601 gcagcaacca tgtatggtgt cggcgttgaa actcgtctga tgggtgcagc taccgccagt
     9661 tctccttcgc cgcaatgggt cgcatggttg caaagtcagg cggctcaggt cgcggggggc
     9721 aatcaggcca ttgaacgtgt tgaagcgcct gcgggttccg aagatgccac attaatgatg
     9781 gcccgcgtgc agcaacatca agggcaagcc tcctacgtgg tgtttggcac acagctggcg
     9841 gcaggtcatc acaacgaaaa attcgatttt gacgagcagg ttctcgctat tgccgtcgaa
     9901 acgctggcgc gcaccgcgct caattttccc tggacgcgag gtatctgatg caggaaatct
     9961 atcgttttat cgacgatgcg attgaagccg atcgccaacg ttataccgat attgccgatc
    10021 aaatctggga tcatccagaa acacgttttg aagagttctg gtcagcggag catctggctt
    10081 cggcgctgga atctgcaggc ttcaccgtta cccgcaacgt aggctatatc ccaaatgcct
    10141 ttattgcttc gtttggtcaa ggcaaaccgg ttatcgccct gctgggagaa tatgacgccc
    10201 tggcaggttt aagtcagcaa gcaggttgcg cgcaacctac atccgtgacg cccggtgaaa
    10261 atggtcacgg ttgcggacac aatttgctgg gaaccgccgc ctttgccgct gcaatagccg
    10321 tcaagaaatg gctggaacaa tatgggcaag gcggcacggt gcgcttttat ggttgtcctg
    10381 gcgaagaagg cggctcgggt aaaacgttca tggttcgcga gggggtattt gatgatgtgg
    10441 atgcggcact cacttggcac ccggaagcct ttgccggtat gttcaatacc cgcacgctgg
    10501 caaacattca ggcatcatgg cgctttaaag ggatcgcagc acatgccgcg aatttccctc
    10561 atttgggacg cagcgccctt gatgccgtaa cgttgatgac cactggcacc aacttcctca
    10621 acgaacatat tattgaaaaa gcgcgcgtac actatgccat cacaaatagc ggcgggatct
    10681 cgcccaacgt ggtccaggcg caggcagaag tgctttatct tatccgcgcc cccgaaatga
    10741 ccgacgtgca gcatatttat gatcgggtcg ccaaaatcgc cgaaggtgcg gcattgatga
    10801 ccgaaaccac ggttgaatgc cgcttcgaca aagcctgttc cagttatctc ccgaatcgca
    10861 ccttagaaaa tgccatgtac catgccctat cccattttgg taccccggaa tggaactccg
    10921 aagaactggc ttttacgaaa caaattcagg ctacgctccc ccccaacgat cggcaaaaca
    10981 gtctgaataa tatcgccgca accggtggcg aaaacggcaa ggcttttgca ctacgtcatc
    11041 gtgaaacggt actggcgaat gaggtcgctc catatgccgc caccgataac gtgcttgcgg
    11101 catcgactga tgtcggcgac gtcagttgga aactgcctgt tgcccagtgt ttcagcccct
    11161 gttttgccgt cggtacaccg ctacatacgt ggcaactggt tagccagggg cgaacatcta
    11221 ttgctcataa aggaatgctg ctggcggcga aaactatggc agcaaccaca ctcaatctct
    11281 tcattgattc agggctattg caagaatgcc aacaagagca tcagcaagtt acggacacgc
    11341 aaccgtatca ctgccctatc ccaaaaaacg tgacactgtc atctttaaaa taacaacaac
    11401 aacgcaaaca caacaaccga ggaatgccca tgagtatgtc atccataccg tcgtcctcct
    11461 aatccgggaa gctctatggc tgggtcgaaa gaattggtaa caaggttccc catccttttt
    11521 tgctctttat ctatttgatt atcgtactca tggtgacgac ggcaattttg tcggcctttg
    11581 gcgtcagtgc gaaaaacccg accgatggaa cgccggtggt ggtgaaaaac ctgctcagtg
    11641 tggaaggatt acactggttt ttacccaatg ttattaaaaa ctttagcggt tttgctccac
    11701 ttggtgcgat cctggcgctg gttttaggtg ccggtctggc ggagcgcgtc ggcttactgc
    11761 cagcgctaat ggttaaaatg gcatcgcatg ttaatgtccg ctacgccagt tatatggtgc
    11821 tgtttattgc ttttttcagc cacatttctt ccgatgcggc gttagtgatc atgccaccga
    11881 tgggtgcgct gatttttctg gcggtgggca ggcatccagt tgcaggttta ctggctgcca
    11941 ttgcaggcgt aggttacggc tttacggcta atttactgat tgtcacaacc gacgtgttgc
    12001 tgtcggggat ccgcacggaa gcggcggctg cgttcaatcc gcaaatgcac gtcagtgtaa
    12061 ttgataactg gtattttatg gccagctccg tagtcgtact gacgattgtt ggcggcctga
    12121 taaccgacaa aatcatcgag ccacggttag gtcaatggca gggaaacagc gatgagaaac
    12181 tgcagacatt gaccgaaagt cagcgttttg gtttacgcat agcaggtgtc gtatcgctac
    12241 tttttattgc tgcgatcgcg ctgatggtga tcccggaaaa cgggatattg cgcgatccga
    12301 ttaatcacac cgtgatgcca tcacccttta ttaaaggtat cgtgccacta atcattcttt
    12361 ttttctttgt ggtttcgctg gcttatggca tcgctacccg cacaattcga catcaggcgg
    12421 atttaccgca tttaatgatt gaaccgatga aagagatggc gggatttatc gtgatggttt
    12481 ttcccctcgc ccagtttgtc gccatgttta actggagcaa catggggaaa ttcatcgccg
    12541 tggggctgac cgatatactg gaaagttcag ggcttagcgg catcccggcg tttgtcggtc
    12601 tggcgttgct ttcctctttc ttatgcatgt ttattgccag tggttccgca atctggtcga
    12661 ttctggcccc cattttcgta ccaatgttta tgctacttgg ctttcacccg gcatttgcgc
    12721 aaatcctctt tcgtattgcc gactcatccg tattgccttt agcgccggta tctccttttg
    12781 ttccactgtt tcttggattc ctgcaacgct acaaaccaga cgcgaaactg ggtacttact
    12841 attcgttagt cttgccctat ccgcttatct ttttggtggt atggctgctg atgttgctgg
    12901 cgtggtatct tgttggtctg ccgataggtc cggggattta cccacgtttg tcttaagaga
    12961 gaacggatgc tgagattact tgaagaaaaa attgccacgc cactgggtcc actgtgggtg
    13021 atttgcgatg agcaatttcg cctgcgggcg gttgaatggg aagagtacag cgaacgcatg
    13081 gtgcagctgc tggacatcca ttatcgcaaa gaaggctatg agcgcatttc tgccaccaac
    13141 ccaggcggtt taagcgacaa gcttagtgat tattttgccg gtaatcttag cattattgat
    13201 acgcttccca ctgccacagg ggggacgcca tttcagcgcg aaatctggaa aactctacgc
    13261 actatcccct gcgggcaggt aatgcattac ggccaactgg ctgaacaatt gggccgccct
    13321 ggcgcggcgc gtgccgttgg tgcggcaaac ggatcgaatc ccatcagcat cgtcgtacct
    13381 tgccatcggg ttattggccg aaacggcacc atgaccggat atgcaggcgg agttcagcga
    13441 aaagagtggt tattgcgcca cgaaggttat cttttgctgt aaacattaaa caatttgtgc
    13501 cagcttgttc acacttttat gtaaagttac ccttaacaac ttaatggttt tcaaatagat
    13561 agacatatat ttacatctaa tatcggaatt ctctgctgtt aaggtttgct tagacttact
    13621 tgctccctaa aaagatgtta aaattgacaa atatcaatta cggcttgagc agacctatga
    13681 tcccggaaaa gcgaattata cggcgcattc agtctggcgg ttgtgctatc cattgccagg
    13741 attgcagcat cagccagctt tgcatcccgt tcacactcaa cgaacatgag cttgatcagc
    13801 ttgataatat cattgagcgg aagaagccta ttcagaaagg ccagacgctg tttaaggctg
    13861 gtgatgaact taaatcgctt tatgccatcc gctccggtac gattaaaagt tataccatca
    13921 ctgagcaagg cgacgagcaa atcactggtt tccatttagc tggcgacctg gtgggatttg
    13981 acgccatcgg cagcggccat cacccgagct tcgctcaggc gctggaaacc tcgatggtat
    14041 gtgaaatccc gttcgaaacg ctggacgatt tgtccggtaa aatgccgaat ctgcgtcagc
    14101 agatgatgcg tctgatgagc ggtgaaatca aaggcgatca ggacatgatc ctgctgttgt
    14161 cgaagaaaaa tgccgaggaa cgtctggctg cattcatcta caacctgtcc cgtcgttttg
    14221 cccaacgcgg cttctcccct cgtgaattcc gcctgacgat gactcgtggc gatatcggta
    14281 actatctggg cctgacggta gaaaccatca gccgtctgct aggtcgcttc cagaaaagcg
    14341 gtatgctggc agtcaaaggt aaatacatca ccatcgaaaa taacgatgcg ctggcccagc
    14401 ttgctggtca tacgcgtaat gttgcctgat ttttccgcat aactcactat ccttctgtca
    14461 tatcattaaa tttttctgat ttattgatct ggcagaaggt tcatcactgt ttcattcacc
    14521 agatatgggt taatctttta attacaaact gcgttgacag ttgttgtaag gagaccctgt
    14581 atggctatgt atcagaacat gctcgttgtt atcgatccta accaggacga ccaaccagca
    14641 ttgcggcgag ctgtttattt acatcaacgg attggtggca aaattaaagc ctttttgccg
    14701 atctatgact tctcatacga aatgaccacc ctgctctccc cggacgaacg taccgctatg
    14761 cgtcagggcg tcatcagcca gcgtacagcc tggatccacg agcaggcaaa atattatctc
    14821 aatgctggcg ttcccattga aattaaagtg gtctggcata accgtccttt cgaagccatc
    14881 attcaggaag tgatcagcgg cgggcacgat ttggtgctaa aaatggcgca ccaacatgac
    14941 cgtctggaag cggtgatttt tacgccaacg gactggcatc tgttacgcaa atgcccaagc
    15001 ccggtgtgga tggtgaaaga ccagccgtgg ccggaaggag gtaaggcgct ggtggcggtg
    15061 aatctcgcca gtgaagagcc gtatcataat gcgctcaatg aaaaactggt caaagagacg
    15121 atcgaactgg cagaacaagt caaccatacc gaagttcatc tggttggcgc ttacccagta
    15181 acgccaatta atatcgcgat tgaactgccg gaatttgagc cgagcgttta taacgatgcc
    15241 attcgtgggc aacatttgct ggcaatgaaa gccctgcggc agaaattcgg cattaatgaa
    15301 aacatgacgc acgtagaaaa aggtctgccc gaagaggtta ttcctgattt ggcggagcac
    15361 ttacaggcgg ggattgtggt tctgggcacg gtaggacgca ccggtatttc agcagcattc
    15421 ctcggcaaca cggcggaaca ggtgattgat catcttcgct gcgatctgtt ggtcattaaa
    15481 cctgaccagt atcagacacc cgttgaactg gatgacgaag aagacgatta acgactcttc
    15541 aataactgac aaggcccgag ttatcgggcc tattttttac ttactgctct tcgccgccaa
    15601 ggaaataaat tcccaacggg attgccagca acacagtaaa gaccaggcta taaacaaaaa
    15661 tcatcgatga ctgaatcaca tacattgatg tcgtccactc agacagcgga atattgtact
    15721 gttctataac gccgccgata gtcgcgcgag tcacaaccga cgccgcaata atgacaaacc
    15781 gccaacaaac caaagagaaa tttcttccct ttcgctgact tcagtttcgc cataatcatg
    15841 atgtaatcct tgtgcagaca aatttcttgt tacctaaacg taacaatacc atgacatttc
    15901 accgttgtct gctctgaaaa cctcacctct tagctggatt tacaatcaac gcattgatta
    15961 gtta
//
LOCUS       DAJTNN010000090        15962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTNN010000090 DAJTNN010000000
VERSION     DAJTNN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.090"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..105)
                     /locus_tag="OO150_002486"
     CDS             complement(<1..105)
                     /locus_tag="OO150_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HCR5516588.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVG"
     gene            complement(110..295)
                     /locus_tag="OO150_002487"
     CDS             complement(110..295)
                     /locus_tag="OO150_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5516589.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            complement(292..2931)
                     /locus_tag="OO150_002488"
     CDS             complement(292..2931)
                     /locus_tag="OO150_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5516590.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3139..4128
                     /gene="ldhA"
                     /locus_tag="OO150_002489"
     CDS             3139..4128
                     /gene="ldhA"
                     /locus_tag="OO150_002489"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5516591.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4239..4661
                     /gene="hslJ"
                     /locus_tag="OO150_002490"
     CDS             4239..4661
                     /gene="hslJ"
                     /locus_tag="OO150_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5516592.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4658..4924)
                     /locus_tag="OO150_002491"
     CDS             complement(4658..4924)
                     /locus_tag="OO150_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5516593.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5198..8722
                     /gene="nifJ"
                     /locus_tag="OO150_002492"
     CDS             5198..8722
                     /gene="nifJ"
                     /locus_tag="OO150_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5516594.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9088..10221
                     /gene="ompN"
                     /locus_tag="OO150_002493"
     CDS             9088..10221
                     /gene="ompN"
                     /locus_tag="OO150_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR5516595.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10362..10796
                     /gene="uspF"
                     /locus_tag="OO150_002494"
     CDS             10362..10796
                     /gene="uspF"
                     /locus_tag="OO150_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5516596.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11353..12267
                     /gene="sitA"
                     /locus_tag="OO150_002495"
     CDS             11353..12267
                     /gene="sitA"
                     /locus_tag="OO150_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5516597.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12267..13094
                     /gene="sitB"
                     /locus_tag="OO150_002496"
     CDS             12267..13094
                     /gene="sitB"
                     /locus_tag="OO150_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5516598.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            13091..13948
                     /gene="sitC"
                     /locus_tag="OO150_002497"
     CDS             13091..13948
                     /gene="sitC"
                     /locus_tag="OO150_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5516599.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            13945..14802
                     /gene="sitD"
                     /locus_tag="OO150_002498"
     CDS             13945..14802
                     /gene="sitD"
                     /locus_tag="OO150_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5516600.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15130..>15962)
                     /locus_tag="OO150_002499"
     CDS             complement(15130..>15962)
                     /locus_tag="OO150_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5516601.1"
                     /translation="DYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSS
                     LPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARRK
                     IHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYDWIQ
                     LQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVGRKNYLF
                     FGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK
                     "
BASE COUNT         3969 a         4062 c         3864 g         4067 t
ORIGIN      
        1 cccgacccgc ccctgcgttc tggcttcatc cagagtcaat gccattacat tgctgcttac
       61 cagcccaaca agaaacgcac aaagaagtaa tgttttcttc atcatcgcct cagtaaagat
      121 cgctacgagt ttcaagcagc tcttcgacat ctttgtctgc cttgatgatg atctcatgct
      181 caattttaac gttcatattg atagtgatcg gttccttggg cgcagcgact tcaatacgcg
      241 gcgtacaggc agccagcata aaagatgacg tcaacgcagc cagtaaaatt ttcattgttt
      301 ttcctcacac tcttttcctt gcggacagtc atttcccggc agacgtgcgt tctgctccaa
      361 ccatgcctgg agattatcgc caaagcgtaa actgcgccac agcgtaaaaa tattctcttc
      421 atgatggtaa ttaaggttta ccgtaccgct tttaccatca acgcgactgg tacctgtaat
      481 gttggcctgc atggttaata aaccgagatt atctaaatta atatttgtcg atgaacggct
      541 aatttccata tagcgcaacc agttaattgc tgaacccgca gtcatattgt ctttcaccac
      601 cgcatctgcg gtgtctttat ccagccgcaa ggtcattggc ccgctatttg ccagccaacc
      661 atctttcact atccattttt cgttgtttag ccataacggc aatgcaccac taaatgcccc
      721 ggacatggcg aattgtttcg gattgacggc gctaaccagt tcgctggatg agagattatt
      781 cagccgcaac agcgccgggt catgttgcgg catacgtaat tgtttcatca gtacgttacc
      841 gcctaacaca tcgacgctaa catcagtcaa cagcaagggt tcttcttcag tccacggata
      901 acgcccttgc aaatcagccg taatattttt cgctgtcacc agattaatca cttcggcaat
      961 tcgcaacgta acggggccgc gagtccccag atgccaggct ccatcggcaa aacggaaagg
     1021 caggacaaaa tcgacaccgt taacctgatt atctggcatc caggcactac cgcctttcaa
     1081 cacgccgtgc cctcccgcgc ggaatccttg ttcaggcgca gcagaaaatg caacctgagc
     1141 atacagttca ccatcgcgta agttcatctt ccagtcgggt ggcaccagcg gctggaatac
     1201 agtcagtgat tgttttggcc accaggcgtt accgcgcaga cgaataccgt cccagcgacc
     1261 atttacccga accgggccaa tctcaccagc atgtaaatcg cctttaaaga ggaaatacgt
     1321 cggatcgcgc ccatcgacgc taaattttaa ggttgatggc ggtaacacac tgccgccagt
     1381 gaacagcgtt tgcccggcgt ccagtgacag cgcgccgctg aaggagggat gctgtgcgtc
     1441 acgtacccag acgacgggct tgtccagaat taatcgcggc ttttctaccg tcatcgtacc
     1501 gtattgtaac tggtcgaaac cggtggagag gtcggtcagc gtaatcgtgc tgtcatgcca
     1561 ttcacctttt cctgcgacat cccaggtggc attcatcggt gtaaaactgc cttttcccca
     1621 gtagcgccac tgccagcggc ccgcgtcagg gagaaaatca ttcgccagcc catccatatg
     1681 cagcacgaaa tcgcccagtt cattttcatg cgcctgcaaa atggcctgca aacgtccgtc
     1741 aacaccacgt tgggtgactt ttacacccgc taaaggccag cggatttcat cgatgtccag
     1801 cgaatcgatg actcttccct ttgaacgaag taacgcgcct ggctcaaagg ccagcgttgg
     1861 gtcagtcaga cttccactta gctgcgcggg taagcgggca tataaaatga ggtccgcctg
     1921 tttcgcttca ccggtcagct gcagaggcag ctggctgtta tccatgctta attttcctgg
     1981 gccaaaattt agcaccgcgt tgcctttacc cgcttgcccc tgggtcagca cactcagtcg
     2041 tccgctgatc agagcgttct caagccctgc ctgccagttg tcgactttga cacccagtcg
     2101 gccactcaaa ggaaaacctg catacggcca actccagcga ccatcgctta cggtcaattg
     2161 ttgacgagta atttgccacg gcaaatcgag caacggatcg ccgttatcac gtgccagcac
     2221 aatcaattgc ccgctatttt cctgccagtc cagctctgca tccaccagtg acggttcctg
     2281 cggcaagttt aacgtcgcag tagcatgccc acttacagga agtccatccg gcacgagcgg
     2341 catagtaaat tcccccacca gtttaaccgg cggctgatat tcaaacgcga tgacatccag
     2401 ttcgctgact gtaagttgtt gccctttcag ctggccttga aatttaactt tttcgccctg
     2461 ataacgcagt tgctggatat cagaggttaa tgcgagagag agttttccct gccattcctg
     2521 ccagggagaa aaaatcagtt tatcgatatt gatccaggtg ttaggcagca tggactgcca
     2581 ctgcgcgagg gtttttggag cagctggcga ttgctccgtc tgcggcaatt tcgccagaca
     2641 agcagaatca agttctaccg tgccgacgtt caataaccag cggctgggat gtgaaaggct
     2701 ggcgttggtg atatgcgcaa gctgacaatc tcccaccaga taacggagat cgggaatgat
     2761 taaaccttta cgcgtaatgc gtgggctttc atctaatgca atacgtgtcc cgagcggtag
     2821 ccagatgccc gccagcgtgg gaacccacag cccgagcgtc atcagcagcg tcaacggcac
     2881 aagaataatc agtaataaca gcgcgagaac ggctttatat ttacccagca tgggtagtta
     2941 atatcctgat ttagcgaaaa attaagcatt caatacgggt attgtggcat gtttaaccgt
     3001 tcagttgaag gttgcgccta cactaagcat agttgttgat gaatttttca atatcgccat
     3061 ggttttcaat tatatttgaa attttgtaaa atatttttag tggcttaaat gtgattcaac
     3121 atcactggag aaagtcttat gaaactcgcc gtttatagca caaaacagta cgacaagaag
     3181 tacctgcaac aggtgaacga gtcctttggc tttgagctgg aattttttga ctttctgctg
     3241 acggaaaaaa ccgctaaaac tgccaatggc tgcgaagcgg tatgtatttt cgtaaacgat
     3301 gacggcagcc gcccggtgct ggaagagctg aaaaagcacg gcgttaaata tatcgccctg
     3361 cgctgtgccg gtttcaataa cgtcgacctt gatgcggcaa aagaactggg gctgaaagta
     3421 gtccgtgttc cagcctatga tccagaggcc gttgctgaac acgccatcgg tatgatgatg
     3481 acgctgaacc gccgtattca ccgcgcgtat cagcgtaccc gtgacgctaa cttctctctg
     3541 gaaggtctga ccggctttac tatgtatggc aaaacggcag gcgttatcgg taccggtaaa
     3601 atcggtgtgg cgatgctgcg tattctgaaa ggttttggta tgcgtctgct ggcgttcgat
     3661 ccgtatccaa gtgcagcggc gctggaactc ggtgtggagt atgtcgatct gccaaccctg
     3721 ttctctgaat cagacgttat ctctctgcac tgcccgctga caccggcaaa ctaccatctg
     3781 ttgaacgaag ccgccttcga tcagatgaaa aatggcgtga tgatcgtcaa taccagtcgc
     3841 ggtgcattga ttgattctca ggcagcaatt gaagcgctga aaaatcagaa aattggttcg
     3901 ttgggtatgg acgtgtatga gaacgaacgc gatctgttct ttgaagataa atccaacgac
     3961 gtgatccagg atgacgtatt ccgtcgcctg tctgcctgcc acaacgtgct gtttaccggg
     4021 caccaggcat tcctgacagc agaagctctg accagtattt ctcagactac gctgcaaaac
     4081 ttaagcaatc tggaaaaagg cgaaacctgc ccgaacgaac tggtttaatg ttgcagctcc
     4141 cctgcaatcc aggggagctg attcagataa tccccaatga cctttcattc tctattctta
     4201 aaatagtcct gagtcagaaa ctgtaattga gaaccacaat gaagaaagta gccgcgtttg
     4261 ttgcgctaag cctgctgatg gcgggatgtg taagtaatga caaaattgct gttacgccag
     4321 aacagctaca gcatcatcgc tttgtgctgg aaagcgtaaa cggtaagccc gtgaccagcg
     4381 ataaaaatcc gccagaaatc agttttggtg aaaaaatgat gatttccggc agcatgtgta
     4441 accgctttag cggtgaaggc aaactgtcta atggtgaact gacagccaaa gggctggcaa
     4501 tgacccgtat gatgtgcgct aacccgcagc ttaatgaact cgataacacc attagcgaaa
     4561 tgctgaaaga aggtgcacaa gtggatctga ccgcgaacca gttaacgctg gcgaccgcga
     4621 aacagacatt aacttataag ctggcggatt taatgaatta atagctgcca cagctcccgg
     4681 cggcaagtga ctgttcgcta cagcgtttgc cgttgggtaa tgcacacatc ccaatcgccg
     4741 tgccatccag ttgacgggca acagaaagcg aaccgccgat cattgcacaa tttgcttctc
     4801 cactactgga catcgacgct tttaaacctg gcgctacgtg cgccgcagtc gcctgctgaa
     4861 caggttcact actacacgcc gacaacaata aagcggcaca ccctacccaa aacgctgctc
     4921 gcatcttttc ttcctctgat cttcaagcca aacgacaccg ccataaataa taggcagcac
     4981 agagggcgac atcgagagct gtcctgcgcg ttgccccgca tttttacttt tttatggcta
     5041 tttttttgcc ctctgtttga tcaaaacatt cattacgctg atgtggggga cacaaaagcg
     5101 aaaatgcaga agaaagccat ttgctaaaat tgaaagatta ctactgggcg cgcagcaatt
     5161 tcgtgcgccc ctcatttgcg caatgtaagg gtgtcatatg attactattg acggtaatgg
     5221 cgcggttgct tcggtggcgt ttcgcaccag tgaagttatc gccatctacc ctattacccc
     5281 cagttccacg atggcagaac aggctgatgc ctgggccgga aacggcttga agaacgtttg
     5341 gggagacaca ccacgcgtgg ttgaaatgca gtcggaagcg ggtgctatcg ctaccgtgca
     5401 tggcgctttg cagacgggtg ccctttcaac atcgtttacg tcatcgcagg gtttgctgct
     5461 gatgatcccg acactgtaca aactggcagg cgaactcaca ccgtttgtcc tgcatgtagc
     5521 ggcacgtacc gttgccacac atgcactctc tatttttggc gatcattccg acgtcatggc
     5581 ggtgcgccag acgggttgcg cgatgttgtg tgcggcaaac gtccaggaag cgcaagactt
     5641 tgctctcatt tcgcacatcg cgacgctgaa aagccgcgtg ccatttattc atttctttga
     5701 tggtttccgc acgtcccacg aaatcaataa aattgtcccg ctggccgatg acacgattct
     5761 tgatctcatg ccgcaggccg aaattgatgc tcatcgcgcc cgggcgctca acccggaaca
     5821 tccggtgatc cgtggtacgt ccgccaatcc tgactcttat ttccagtctc gcgaagccac
     5881 caacccctgg tacaacgcag tctatgacca tgttgaacag gcgatgaatg atttctctgc
     5941 cgcgacaggt cgtcagtatc agccgtttga atattacggg catccgcaag ctgaacgggt
     6001 gattatcctg atgggctcag ccattggcac ctgtgaagaa gtggttgatg aattgctaac
     6061 ccgtggcgaa aaagtcggcg tgctgaaagt tcgcctgtac cgccccttct cagccaaaca
     6121 cttgctacaa actctaccgg gatccgtacg cagcgtggcg gtactggaca gaaccaaaga
     6181 acccggtgcc caggcagaac cgctctatct ggatgtgatg accgcactgg cagaagcctt
     6241 taataatggc aagcgcgaaa ctctgccccg tgtcattggt gggcgctatg gtctttcatc
     6301 caaagaattt ggcccggact gtgtactggc ggtatttgcc gagctcaacg cggctaaacc
     6361 gaaagcgcgc tttacggttg gtatttacga tgatgtaacc aatctgtccc tgccgttgcc
     6421 ggaaaacacc cagccaaact cggcgaaact tgaagccttg ttttatggcc ttggtagtga
     6481 tggcagcgtt tccgcgacca aaaacaatat caagattatc ggtaattcca cgccgtggta
     6541 cgcacagggc tattttgttt acgactccaa aaaagcgggc ggcctgacgg tttctcacct
     6601 tcgcgtgagc gaacagccga ttcgttccgc ttatctcatt tcccaggctg attttgttgg
     6661 ctgccaccag ttgcagttta tcgataaata tcagatggct gagcgtttaa aacctggcgg
     6721 cattttcctg ctcaacacgc cgtacagcgc agatgaagtg tggtcgcgct tgccgcaaga
     6781 agttcaggcc gtgttaaacc agaaaaaagc gcgcttctat gtgattaacg cggcgaaaat
     6841 cgcccgcgaa tgtggcctgg cggcccgtat taataccgtc atgcagatgg cttttttcca
     6901 tctgacgcaa attctgcctg gcgatagcgc cctcgcagaa ttgcagggtg cgattgccaa
     6961 aagttacagt agcaaaggcc aggatctggt ggaaggcaac tggcaggctc tggcgctggc
     7021 gcgtgaatcc gtagaagaag ttccgttgca accggtaaat ccgcacagcg ccaatcgacc
     7081 gccagtggtt tccgatgccg cccctgattt cgtgaaaacc gtaaccgcag cgatgctcgc
     7141 agggcttggt gatgccctcc ccgtttcggc gctgccgcca gacggcacct ggccgatggg
     7201 gaccactcgc tgggaaaaac gcaatatcgc cgaagagatc cccatctgga aagaggaact
     7261 ctgtacccaa tgtaaccact gcgtcgccgc ttgcccacac tcagctattc gcgcaaaagt
     7321 ggtaccaccc gaagcgatgg aaaacgcccc tgccagcctg cattcgctgg atgtgaaatc
     7381 gcgtgatatg cgcgggcaga aatatgtctt gcaggtggct ccggaagatt gcaccggttg
     7441 taacctgtgc gtcgaagttt gcccggcgaa agaccgtcag aatccagaga ttaaagccat
     7501 caatatgatg tcgcgcctgg aacatgtcga agaagagaaa atcaattacg atttcttcct
     7561 caacctgcca gaaatcgacc gtagcaaact ggaacgtatt gatattcgta catcgcagct
     7621 gattacaccg ctgtttgaat attccggtgc ttgctccggt tgtggcgaga cgccgtatat
     7681 taaattgctg actcagctct atggcgaccg gatgttgatc gctaacgcca ccggctgttc
     7741 ttcaatttat ggcggtaacc tgccctctac accgtatacc accgatgcca acggtcgtgg
     7801 gccggcatgg gcgaactctc tatttgaaga taatgccgaa tttggccttg gtttccgcct
     7861 gacggtcgat caacaccgtg tccgcgtgct gcgtctgctg gatcaatttg ccgataaaat
     7921 cccggcggaa ttactgacgg cgttgaaatc agacgctacg ccagaggttc gtcgagaaca
     7981 ggtcgcagct ttacgccagc aactcaacga tgttgccgaa gcacatgaac tgctacgtga
     8041 tgcagatgca ctggtggaaa aatcaatctg gctgattggt ggtgatggct gggcttacga
     8101 tatcggcttt ggcggtctgg atcatgtatt gagtttgacg gaaaacgtca acattctggt
     8161 gctggatacg caatgctatt ccaacaccgg tggtcaggcg tcgaaagcga caccgctggg
     8221 tgcagtaact aaatttggcg agcacggcaa acgtaaagcg cgtaaagatc ttggcgtcag
     8281 tatgatgatg tacggtcatg tttatgtggc gcagatttct ctcggcgcgc agctgaatca
     8341 gacggtgaaa gcgattcagg aagcggaagc gtatccggga ccatcgctga tcattgctta
     8401 tagcccgtgt gaagagcatg gttacgatct ggcactcagc catgaccaga tgcgccaact
     8461 cacagctacc ggcttctggc cgctatatcg cttcgatcct cgtcgtgccg atgaaggcaa
     8521 actgccgctg gcgctggatt cacgcccgcc gtcagaagca ctggaagaaa cgttacttca
     8581 cgagcaacgt ttccgtcgtc tgaattcgca gcagccagaa gtggcagaac agttatggaa
     8641 agatgctgca gctgatttgc aaaaacgcta tgacttcctg gcacaaatgg ccggaaaagc
     8701 ggaaaaaagc aacaccgatt aaatgctctg gataaggatt atccaattct aaaaaaaagc
     8761 ccggacgact gttcgggctt gtctttttat atgttggaaa atcagtggca atgcaatggc
     8821 ccaacagaaa ataaaatctg tgacaataaa ggcatataac ccgcgcagaa taacgtatag
     8881 aaaattaaaa gtataatttt tattttgtat gtattatctt cgatacattc accagactta
     8941 ttatcatttc agaagaatta tttgattaag gttttactta aggcgtaaca aatgatattt
     9001 atcggctaac tgaacttctc ctttaggatg ttttcactcc ccggaatggg gataaaaggc
     9061 aaataaaata actaaaggat ttattcaatg aaaagcaaag tactggcact tttaattcct
     9121 gccctgctcg ccgcaggtgc tgcacatgca gccgaagttt ataataaaga cggcaacaaa
     9181 ttagatctgt atggcaaagt tgatggcctg cattattttt ctgataattc agcgaaagat
     9241 ggcgatcaga gctatgcgcg tctgggtttt aaaggcgaaa cccaaattaa cgatcaactc
     9301 actggctacg gtcaatggga atacaatatt caagcaaaca acactgaatc ttcaaaaaac
     9361 cagtcatgga cccgtctggc atttgccggg ctgaaatttg cagattacgg ttctttcgat
     9421 tacggacgta attatggcgt aatgtacgac atcgaaggct ggaccgatat gctgcctgaa
     9481 tttggcggtg actcttatac caatgcagac aactttatga ctggtcgagc caatggcgtc
     9541 gcgacttatc gtaatactga tttcttcggt ctggtaaatg gtctgaactt cgcggtgcag
     9601 tatcaaggta acaacgaagg agccagtaat ggtcaggaag gcaccaacaa cggacgtgat
     9661 gttcgccatg aaaacggtga cggctggggt ttttccacaa catatgattt aggcatgggc
     9721 tttagcgctg gtgcggcata cacctcttct gaccgcacca atgaccaggt taaccatact
     9781 gcggcgggtg gtgataaagc agacgcgtgg actgctgggc taaaatacga tgctaacaat
     9841 atttacctag caaccatgta ttcagaaacg cgtaatatga ccccgtttgg cgacagcgat
     9901 tatgctgtgg caaacaaaac ccagaatttt gaagtcactg cccagtacca gtttgatttt
     9961 ggtctgcgtc cggcagtctc tttcctgatg tctaaaggcc gtgacctgca cgctgccggt
    10021 ggtgcagaca acccggcagg tgttgatgat aaagatctgg ttaaatacgc cgatgttggc
    10081 gcgacttact atttcaataa aaacatgtcc acctacgttg actataaaat caacctgttg
    10141 gatgaagatg acagcttcta cgctgccaat ggcatctcta ccgatgatat tgtcgcttta
    10201 ggtctggttt atcagttcta aatcctcctg cccgctgtta tggcgggctt tttctgctta
    10261 ttcttcctcc tttgatctaa attaaaaatg tgaactccat cattacacaa aaagtgtcat
    10321 ctggcgttac actttatgcg gatactaaaa caggaggttt tatgaacaga acgattcttg
    10381 tccctatcga tatttccgat tcagaattaa ctcaacgcgt gattagccac gttgaggcag
    10441 aggcaaagat tgacgatgca gaggttcatt tcctgacggt aataccttca ctgccctact
    10501 atgcctctct gggtttagcg tattccgcag aattaccggc aatggatgac ctgaaagcgg
    10561 aagccaaatc gcaactggaa gagatcatta aaaaatttaa actgccaacc gacagagtgc
    10621 atgtccatgt tgaggaaggc tcgcccaaag accgcattct ggaattggcg aagaagatcc
    10681 ccgctcatat gatcatcatt gcttcccatc gaccggatat caccacttat ctgctcggtt
    10741 ccaacgccgc agctgtagtg cgtcacgcag agtgctccgt gctggttgtg cgctgacact
    10801 aacgcccgca cattgctgcg ggctttttga ttcatttcgc aaatgtgctg acattttccc
    10861 ctctaatccg taccatacac gccactgttt ttatatcaga tttcttatgc tgggcattcc
    10921 ggcatgacgc atactcttct gatgccatat aacgaattga gtcgctttta aatgtcgcaa
    10981 aatcaagaaa ttagtaagaa agaacaatac aacctgaaca agtaagggca aaaatcacaa
    11041 ctatctgact cataagtatt ttatttattt ttcaatatgt taaacattat cagcgacaac
    11101 aataagctac gataacccac tctttttttg ccccattttt gccatcaaca attttcctca
    11161 taaagtcatt tcaaagccta atagctgctc tacttttttc ctcaatatca tgtcgccagc
    11221 aggaaacaac ctaatccctg ttttaggcct caaagctgtt aatgataatt attttcattt
    11281 tgtataggtt atgctataaa acatagccgt tgctatgttt gttctaattt tgctcactat
    11341 aggtactaaa ttatgcactc aataaaaaaa gtaaccatgc tcttggggtg cctcgccctc
    11401 acctgctcga tcgcatttca ggcaagtgca actgaaaaat tcaaggtcat tacaacattt
    11461 accatcatcg cggatatggc caaaaacgtg gctggagatg ctgcagaagt ctcatccata
    11521 accaagcctg gtgcagaaat tcatgagtat cagcctaccc ctggcgatat taaacgtgca
    11581 cagggggcac aactgattct cgccaatggt atgaatctgg aattgtggtt ccaacgcttt
    11641 taccagcatc tcaatggggt tccagaagta attgtctctt cgggtgtgac gccagtaggg
    11701 atcaccgaag gaccctatga gggcaaacct aatccccatg catggatgtc gccagataat
    11761 gctctgattt acgtcgataa tattcgtgat gcgttgataa aatacgatcc ggcaaatgca
    11821 caaacctacc aacgtaatgc cgatacttat aaagccaaga ttacccaaac ccttgccccc
    11881 ctgcgtaagc agattacgga actccctgag aatcagcgat ggatggtcac cagtgaaggg
    11941 gctttttctt atctcgcacg cgatttgggg ctaaaagagc tttatctgtg gccgattaat
    12001 gccgatcaac aaggaacacc gcagcaggta cgtaaggttg ttgatatagt taagaaaaat
    12061 catatcccgg cagtctttag cgagagtacg atttccgata aaccagcgcg tcaggttgcg
    12121 cgtgaaaccg gcgcacacta cggcggtgtg ctctatgtcg attccctgag cacagaaaac
    12181 ggcccggtac ctacgtatat cgaccttctt aaggttacta ccagtacgct ggtacaggga
    12241 attaaagccg ggaaaaggga gaaataatga tgcaatctgc aggcattgtc gttaatgatg
    12301 ttactgtcac ctggcgtaac gggcacacag cactgcgcga tgcatccttc acagtaccaa
    12361 gtggatctat tgccgctctg gttggggtaa acggttccgg aaaatcgacg ttgtttaaag
    12421 cgattatggg atttgtgcgt ctgaccagcg gaaagatatc tgttctgggt attcccacgc
    12481 gacaggcgct acagaaaaac ctggttgcct acgtccctca gtcagaagag gttgactggt
    12541 catttcctgt actggtggaa gatgtggtga tgatgggccg ctatgggtat atgggtatgt
    12601 tgcgtatagc caaaaagagg gatcgtcaga ttgtcaccga tgcgctggaa cgtgtcgata
    12661 tggtggattt tcgccatcgt caaatcggcg agctttccgg tggacaaaag aaacgcgtct
    12721 ttctggcgag agcgattgca cagcagggtg atgtgatcct gctggatgaa ccttttaccg
    12781 gcgttgatgt gaaaaccgaa gcgaaaatca tcagcctgtt gagggagtta cgcgcggaag
    12841 gcaaaacgat gcttgtctca acgcacaatc tcggctcagt cacgacattt tgcgattata
    12901 cggtcatggt caaaggcacc gtcctggcaa gcgggccgac agacaccact tttacagccg
    12961 agaacctgga gctggctttt agcggcgtac tgcgccatgt cacacttaac ggctcggaag
    13021 aaagcattat tactgacgat gaacgccctt ttgtggcaca tcgaccgtca gcggtgcaga
    13081 gggaagcaag atgaacgtgc tactggaacc cttcagctat gaatatatgc tcaatgcgat
    13141 gtgggtctcg gcaatggtcg gcggtctctg cgctttcctg tcatgctatc tgatgctaaa
    13201 aggctggtcg ctgattggcg atgcgctgtc gcactctatt gtcccgggtg ttgcgggggc
    13261 atatatgctg gggcttccgt tttctctggg agccttcttt tccggcggac tggcagcggg
    13321 cagcatgctg tttcttaacc aacgcacccg cttaaaagaa gatgccatca tcggcctgat
    13381 cttctcgtcc ttttttggcc tggggctgtt tatggtgtca ttgaacccga catccgtgaa
    13441 cattcagacc atcgtcctgg gcaatattct ggcaatcgat ccggcggata tcctgcaact
    13501 cactatcatt ggcattctct cgatcatcgt gctttttttc aaatggaaag atcttatggt
    13561 gacctttttc gacgagaatc acgcccgtgc tattggatta catccgggca gattaaaaat
    13621 cctcttcttc accctgctgt cggtttcgac cgttgcggcg ctgcaaacgg ttggtgcctt
    13681 tctggtgatc tgtctggtgg tgactcccgg tgcaacagca tggttgttaa ccgatcgttt
    13741 tccccgcctg ctcataattg ctgtaactat tggcagtgta accagtttcc tcggcgcgtg
    13801 ggtcagttac tttctggacg gcgcgacagg tggaatcatc gtggtggcgc agacactgct
    13861 gtttctttta gcatttgtat ttgcgcccac ccatggactt ttggcaaatc gtcggcgtgc
    13921 gcataaagcg ctggaggatc gctcatgatg gcattattac tggagccact gcagttcacc
    13981 tttatgagtc acgcactgct gatttcactg gtagtttcca taccctgtgc gctgctgtcg
    14041 gtctttctgg tcctgaaagg ttgggcactg atgggtgacg cgatgagtca tgcggttttt
    14101 cctggcatcg tgcttacctg gattctgggg ttacctctgg ctacaggggc attcgtcgcc
    14161 ggagtattct gcgcggtggc gacgggatac ctgaaagaca acagccgaat caagcaagat
    14221 acagttatgg ggattgtttt ttctggcatg tttgccgcag gtctgatctt gtatattgca
    14281 atcaaaccag acgtacatct tgaccatatt cttttcggcg acatgctcgg gataaccatc
    14341 ggcgatataa tccagacggt gattattgcc gggctggtta cacttgttat tagcgtaaaa
    14401 tggcgggatt ttttgctgtt cagctttgat tatcaacagg cgcaggtaag cggtttgcat
    14461 acgagatggc tgcattatgg gctgctgtgc atggtttccc tgaccattgt ggcgacgctg
    14521 aaagcagtag gtatcattct ctctatttct ctgctcattg cgcctggcgc cattgcagta
    14581 ctcctgacac aacgctttca tattgcgctc ctactggcga ctggcatatc ggtaatagtg
    14641 tcaatgactg gtgtctggct gtcctttttt atcgacagcg caccagctcc gacgattgtc
    14701 gtcttattcg cggtcgtgtt catcatgacg tttaccgtta ccagcatcaa cgcacgcaca
    14761 aagggaaacg cccatacaca ggatctgtta tcacccaatt agcacaatat ccgaggacag
    14821 catcgtccgc cagcgtccgc cagcctacag aagttcggaa agccatggaa ggtcgttggg
    14881 aggaggtacg aattctatca tgcaaaaaat acgtaaaatc gataacgcct agaaatcatt
    14941 caatactcgc actatcgaaa gtttaccagc caaccgcggc acgttcctgc atacgacgtg
    15001 ccgcagcttc tgttattcca cgataaaatt cctaaccgaa ttcaaatcct tcagactatt
    15061 cagctcttcc ttcagctccc gctgacggcg ataaatcgta agcgccccga gaagtacgta
    15121 gcgtaaggat tattttacag acgagaagtt ccagggcagc agttcatgca cttggttcga
    15181 cggccagtca ttgagcttct cgatcacttc gcgcaaccag tcctccggct ccacttcgtt
    15241 ctgtttgcag gtgaccagca gactgtagat gatcgccgca ctttctcctc ccttgtctga
    15301 gccgaaaaag agataatttt ttcttccaac cgccaccgat cgtaacgcgt tttcaccgat
    15361 gttgttgtct atttccaccc agccgtcacg acagaactcg ttcagcgcat tccagtgatt
    15421 caggatatag tcgaacgcct tcgccatctc cgcatgtttc gacagcgttt tcctctgcaa
    15481 ctgtatccag tcgtacaatg actgcatcaa ctggacgctt ctggcttttc tgactgcaag
    15541 ccgttcctct gccggactgc cacgtatctc cgcttctatg tcgtataact ctgctatccg
    15601 tctgagcgct tcctgagtca tttctgtcgg acggcgcaca tcctcgtcat ggatttttcg
    15661 gcgggcgtgg gccaggcacc cggcttcctt cacccggccc gtttcgtaca gtacgttata
    15721 acctgcatag gcatcagcct gcagtacgcc ctgatacttt gccaggtgga gctgcggatg
    15781 ttctcctttg cgatctgccg aatacgcgaa ccagacggct gccggcaggg atgaacccgc
    15841 attacgatca tccctgacgt atacccacag acgacccgtt ttcgtctttc cgttccccgg
    15901 ggccagtact ttcaccggag tgtcatctgc gtgcaccttt cctgcctcca gaacatagtc
    15961 at
//
LOCUS       DAJTNN010000091        15691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTNN010000091 DAJTNN010000000
VERSION     DAJTNN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.091"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            131..379
                     /locus_tag="OO150_002500"
     CDS             131..379
                     /locus_tag="OO150_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5516602.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            357..1487
                     /locus_tag="OO150_002501"
     CDS             357..1487
                     /locus_tag="OO150_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5516603.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRRLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1533..2171)
                     /gene="ompW"
                     /locus_tag="OO150_002502"
     CDS             complement(1533..2171)
                     /gene="ompW"
                     /locus_tag="OO150_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5516604.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2411..2500
                     /locus_tag="OO150_002503"
                     /pseudo
     CDS             2411..2500
                     /locus_tag="OO150_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2528..3271
                     /locus_tag="OO150_002504"
     CDS             2528..3271
                     /locus_tag="OO150_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5516605.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            3301..3840
                     /locus_tag="OO150_002505"
     CDS             3301..3840
                     /locus_tag="OO150_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5516606.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3945..4343
                     /gene="yciA"
                     /locus_tag="OO150_002506"
     CDS             3945..4343
                     /gene="yciA"
                     /locus_tag="OO150_002506"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5516607.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4383..5111)
                     /gene="tonB"
                     /locus_tag="OO150_002507"
     CDS             complement(4383..5111)
                     /gene="tonB"
                     /locus_tag="OO150_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5516608.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTALARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            5326..5622
                     /locus_tag="OO150_002508"
     CDS             5326..5622
                     /locus_tag="OO150_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5516609.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5922..7175
                     /gene="kch"
                     /locus_tag="OO150_002509"
     CDS             5922..7175
                     /gene="kch"
                     /locus_tag="OO150_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5516610.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7230..7403)
                     /locus_tag="OO150_002510"
     CDS             complement(7230..7403)
                     /locus_tag="OO150_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5516611.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7546..9006
                     /gene="cls"
                     /locus_tag="OO150_002511"
     CDS             7546..9006
                     /gene="cls"
                     /locus_tag="OO150_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5516612.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9041..9370
                     /locus_tag="OO150_002512"
     CDS             9041..9370
                     /locus_tag="OO150_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5516613.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9423..10427)
                     /gene="oppF"
                     /locus_tag="OO150_002513"
     CDS             complement(9423..10427)
                     /gene="oppF"
                     /locus_tag="OO150_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5516614.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10424..11437)
                     /gene="oppD"
                     /locus_tag="OO150_002514"
     CDS             complement(10424..11437)
                     /gene="oppD"
                     /locus_tag="OO150_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5516615.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTTVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLCACFKPVE
                     ELL"
     gene            complement(11449..12357)
                     /gene="oppC"
                     /locus_tag="OO150_002515"
     CDS             complement(11449..12357)
                     /gene="oppC"
                     /locus_tag="OO150_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5516616.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12372..13292)
                     /gene="oppB"
                     /locus_tag="OO150_002516"
     CDS             complement(12372..13292)
                     /gene="oppB"
                     /locus_tag="OO150_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5516617.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13378..15009)
                     /gene="oppA"
                     /locus_tag="OO150_002517"
     CDS             complement(13378..15009)
                     /gene="oppA"
                     /locus_tag="OO150_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5516618.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            15307..15603
                     /locus_tag="OO150_002518"
     CDS             15307..15603
                     /locus_tag="OO150_002518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516619.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSFSIEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
BASE COUNT         4039 a         3797 c         3681 g         4174 t
ORIGIN      
        1 cgttaccagg caaaatacat ttcagttatc tggcaaaaac tgatcccgaa tcaggctgat
       61 ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc atgattagcg
      121 aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg aattcagtga
      181 accggtccct actccgagta cgttaagtaa atacgctaaa gccggaatga tatttcctct
      241 ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg tcggaatggt
      301 aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac tggaagatgg
      361 cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt gacaaaagaa
      421 ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac ggtctcggca
      481 gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt cttgcccggc
      541 agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc ggcggttcat
      601 caactgttac catttttctg aataattaca gaaaaattca acaggaaaga tatgaaaacg
      661 gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta tttgatgaac
      721 gttttggcac cagacggtta gatgccataa ccgtaaaaga tgtggtatca gtactggaag
      781 agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta ctgatcgatg
      841 ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct gcagaatcgg
      901 caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag tggatgatga
      961 tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg ctggcactga
     1021 tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc cgggatggtt
     1081 atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg gctctgcgtt
     1141 gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat tgcgttctta
     1201 gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc gggatggtta
     1261 agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat tacaactggc
     1321 gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag cgattgttca
     1381 gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa atgaccgata
     1441 tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga ccagttttgc
     1501 agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc ggagaacata
     1561 aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga tttgtactta
     1621 gccgtggtat cgatatccat gtaccagact gacatgttaa ccaaccagtc acggttaatc
     1681 agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact gagatcggaa
     1741 agccctgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt gtagttaata
     1801 cctgccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca ctgcgccatc
     1861 agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt gccgatttta
     1921 tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc catataagta
     1981 aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact tcctaacgta
     2041 ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa aaattcgcct
     2101 gcttcatgcg caaaggcact gccagagaga agagttgtta ctgccaaagc cgccactgtt
     2161 aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata tacctatatt
     2221 ggagtaacaa atgatcctac atagatcaca ttacatatga taattcaccc tttattgatc
     2281 tggattaatt tagaagttgc attgagaatc ctggttaacg tgatttaaat caatttttaa
     2341 atattttcgc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc ccacgcctga
     2401 aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac ctgatgctgc
     2461 cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata aaactcaagg
     2521 agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt tcttccgtaa
     2581 tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag tggtgttagg
     2641 gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg tgcccgttag
     2701 cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc aacaaatttt
     2761 gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca ttctcgccgg
     2821 aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg cgctgcgagc
     2881 tattggtgcc agtgcaccga tattgccaaa gttatttatt ctgatttttc tgactaccct
     2941 tttagtacag attggcatca tgctagtggt cgttccggga attatcatgg ccattttact
     3001 ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct cgatgcgtag
     3061 cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac tgagctggtt
     3121 gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa ccccggaaat
     3181 tggtgccgta ctggcgaaca ccttgagcaa cctgatttca gccgtattgc tcatctatct
     3241 gttccgtctg tatatgttga ttcgccaata accttaagca tttgattacg gaatcgtaaa
     3301 atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta caagatttat
     3361 gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct tatctatagc
     3421 tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct ggtggtcgtc
     3481 ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa ggttacagtc
     3541 atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa aaagccgcta
     3601 attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc gaagctgaat
     3661 ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat cgcattctgg
     3721 ctgccgcaaa atatttgggt caactttaaa gtctttggac tgaccgccct taccttaatc
     3781 ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga taaatcctaa
     3841 ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc aggaaaatca
     3901 tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct acaacacata
     3961 acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat accaatgcca
     4021 atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc gctattctgg
     4081 cgaaagaaat tgcccacggt cgcgtagtga ccgtgcgggt tgaaggaatg actttcttac
     4141 ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag aaagggacga
     4201 catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa ccaatcgggc
     4261 aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct gaaggaaaac
     4321 ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt ttgactttct
     4381 gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac aatcccactg
     4441 cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc acgctcaaac
     4501 atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc cggcgtgacg
     4561 tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc cggatactgc
     4621 ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg cttgctggtt
     4681 gcagccgttg ctgtacttga tgtcaggcgt gccagtgccg tattttcaaa cggtgatgcc
     4741 ggacgcgact ctacgggttt gacatcgcgt tttggctgct cctgtacctt tttcaccggc
     4801 ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc ttcttttggc
     4861 ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg tggcggctga
     4921 acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat tggctgcgca
     4981 ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc cacaacagca
     5041 ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa atcaagggtc
     5101 attgaagtca taatcatttc agtagaaaaa ccaagcctcg attttaaatg caaatagcaa
     5161 tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg ccgtaattaa
     5221 ccttacttta tgcatggtct taacaaagac atcatctttc cgttgcagat cttcgccctt
     5281 tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt gtatgttatt
     5341 tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc ggcacattta
     5401 gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc aatgccagcg
     5461 gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat tgctgaattt
     5521 gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc ggcaggagtc
     5581 tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat tcaatattta
     5641 agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg gtcagtgcat
     5701 tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt gagcatattc
     5761 aggaaaaatg acctttcctg aggtacagtt gctcgctcaa aacctttgat tactgcggat
     5821 aaagtcatct actattgttt aacacattca cgcgctgcat gtatgttcag aaaatgtgca
     5881 cgctaagata attaaacaaa attgtggtta cagaggtatc agtgagtcac tgggctacat
     5941 tcaaacaaac agcaacaaat ttatgggtga ccctacggca cgacattctc gcgctggccg
     6001 tctttttaaa tggattgctt atttttaaaa caatctatgg tatgtcggtc aatttgctag
     6061 atattttcca tatcaaagca ttttcagagt tggatctttc cttgctggca aacgccccac
     6121 tatttatgct cggcgtcttt cttgtcctga actccattgg cttactgttc cgggcaaagc
     6181 tcgcatgggc aatcagtatc attttgttgt tgatagcgct aatttatacc ctgcattttt
     6241 atccctggtt gaaatttagt attggatttt gcatttttac gctggtgttt ttgctgatac
     6301 tgcgcaaaga cttctcccac agtagcgccg cagccgggac aatttttgca tttattagtt
     6361 tcacgacatt actgttttac tcaacctacg gtgcgcttta tttaagcgaa ggttttaatc
     6421 cgcgaataga aagtttgatg accgcgttct atttttcgat agaaaccatg tcaaccgtcg
     6481 gctatggcga tattgtccct gtttctgaat cagcacgatt gttcactatt tcggtcatta
     6541 tttccggtat taccgttttt gccacatcta tgacctcaat ttttggtccg cttatccgcg
     6601 ggggattcaa caaacttgta aaaggaaaca atcatacaat gcatcgtaaa gatcatttta
     6661 ttgtttgcgg acattcgatt ctcgccatca atacgattct gcaactgaat caacgcggac
     6721 aaaacgtaac ggttatcagc aacttgcctg aagatgatat caagcaactt gagcaacgct
     6781 taggcgataa cgctgatgtt atccccggtg acagtaatga cagttcagta ttaaagaaag
     6841 cgggaatcga tcgttgcctg gccattctgg cgctgagtga taacgatgca gataacgcgt
     6901 ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa aacagttctc gccgtcagtg
     6961 atagcaaaaa cctgaataag attaagatgg tacatccgga tatcattctc tcaccgcaac
     7021 tgtttggcag cgaaattctg gcgcgagttt taaatggtga agagattaat aatgatatgc
     7081 tcgtttcaat gttgttgaac tccggtcatg gcattttcag cgataacgat gaacaagaaa
     7141 cgaaagctga cagtaaagaa tcggcgcaaa aatagagttt aaagatggag gagtaaactt
     7201 attgggcacc gcaatggtgc ccagtacatt tagatattgt agatcgcaaa caataacgag
     7261 ttacgctgtt tgtttagaat gcacttcctg atgctgtgga tacgcatatt tcggcgcgat
     7321 tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac gctgcatgcg ccagcgcccc
     7381 acttccgttc tactccgctt catgttaact actctatgca ataacagaat ggtcattata
     7441 cccccgcccc cgacgctgac cagtgctttt cccgcgtttt tatttgccag atgaatccat
     7501 atgcgtaaac tcataacaat gcgctttcaa aaggatttct aatctatgac aaccgtttat
     7561 acgttggtga gttggttggc cattctggga tactggttgc tcattgcagg cgtaacttta
     7621 cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg cctggctgtt gattatttac
     7681 attctgccgt tagtcggaat tattgcctat cttgccgttg gcgagctcca tttaggcaaa
     7741 cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg caaaatggct taacgacctt
     7801 aaagcctgta agcatatctt cgccgaagaa aatagcagtg tcgctgcgcc attattcaag
     7861 ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga atcagctaca actgatgacc
     7921 gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc agctcgcgcg ccataatatt
     7981 gagattgtgt tttatatctg gcagcccggc ggcatggcgg accaggtggc tgaatcatta
     8041 atggccgctg cgcgacgcgg cattcattgc cgattgatgc tcgactccgc cgggagtgtg
     8101 gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg ccggtattga agtggtcgaa
     8161 gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta tggacctgcg ccaacatcgc
     8221 aagatgatca tgatcgataa ttacatcgcg tacaccggca gcatgaatat ggtcgatcct
     8281 cgctacttca aacaagatgc gggcgtaggg caatggattg atctgatggc gcgtatggaa
     8341 ggccccatcg ccaccgcgat ggggattatt tattcctgcg actgggagat tgaaaccgga
     8401 aaacgtattc tgccgccacc accagatgtc aatattatgc cgtttgaaca ggccagcggt
     8461 cacaccattc acacaattgc ttctggcccc ggctttccgg aagatctcat tcaccaggca
     8521 ttattgactg cggcttattc ggcgcgtgaa tatttgatca tgaccacgcc ctactttgtg
     8581 ccaagcgatg atttacttca tgcgatttgc acggcggcac agcgcggggt ggatgtcagt
     8641 attatccttc cgcgaaaaaa tgactcgatg ctggtcggct gggccagtcg cgcattcttt
     8701 acggaacttc ttgctgctgg ggttaaaatt tatcagtttg aaggcgggtt actgcatacc
     8761 aagagcgtgc tggtcgatgg cgaactaagt ctggttggca ccgttaacct tgatatgcgt
     8821 agtctgtggc tgaatttcga gattaccctg gcaatcgacg ataaaggttt tggtgcagac
     8881 cttgccgccg ttcaggacga ttatatttcg cgttcacgtc tgctcgatgc ccgtttatgg
     8941 ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt tttacttctt cagcccgttg
     9001 ctgtaaaacg tctccaacag aggttaaaca ggtagtgact atggatatgg atctaaacaa
     9061 tcgcctgact gaagatgaaa cgcttgagca ggcctacgat atttttctcg aactggctgc
     9121 cgacaatctc gacccagctg atgttctgct gttcaatctt cagtttgaag agcgcggcgg
     9181 cgcggagtta ttcgatccgg tggaagactg gcaggaacat gttgatttcg atttgaaccc
     9241 ggactttttt gccgaagtgg tgattggtct ggcggacagt gaggatggtg aaatcaacga
     9301 tgttttcgcc cgcattctgt tatgccgcga aaaagatcac aaactttgcc atatcatctg
     9361 gcgggaataa ataatcgctt tcggcgtgaa aaagggctga caactgtcag cccttattgt
     9421 gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc tgaaactccc ttccagcaca
     9481 ggacgtgttt tggcgcactc cggaccggca atcgggcaac gggtacggaa aacacaaccg
     9541 gaaggcgggt tgatcggcga cggtaattcc ccttccagta actggatggt tttgttcttc
     9601 tccagatccg gatcaggtat ggggactgcc gacatcaatg ccctggtgta aggatgtagt
     9661 ggattgtggt agacctcatc ataggtcccc agttctaccg catggccgag atacatcacc
     9721 aacacacgat cggaaatgtg ttttaccacg gccaggtcat gagcaataaa aattaatgac
     9781 aatcccatct cacgttgcag ctgctggagc aggttgacca cctgcgcctg aattgacacg
     9841 tccagcgccg acaccggctc atcacagata atcagcttcg gttcaagaat aagagcacga
     9901 gcaatcccga tacgctggca ctgcccacca gagaactcat gcggatagcg gttaatcagg
     9961 ttaggcaata acccgacttt cagcatcatc gccttcacgc gctcgcgaac ttcctgacgt
    10021 gacattttcg gatgataagt acgcagtggt tcagcgatga tctcgccgat ggtcatgcgc
    10081 gggtttagcg atgccaacgg atcctggaaa atcatctgaa tatcactgcg aacggcacgc
    10141 cattcatcgg gcttaatgcc cagcaactct ttacctaacc aggcaacatg accgtcggtc
    10201 gctttaacca ggccaatgat ggcgcgagca aaggtggact taccgcatcc cgattcccct
    10261 accacaccta atgtttcccc ttcatacagg cgaagcgtga caccatcgac ggctttgagc
    10321 gttttcggcg gttgccagaa ccactgtttg ccatctttga tttcaaagtg cactttcaga
    10381 tcggcgattt caaggaggac ttttcttcct tcggttacag cattcataac agttcctcca
    10441 ccggtttaaa gcaagcacac agacggccaa gcgtaaactc ttccagcggc ggtgcgctac
    10501 tacaaatttc catcgcatgc ggacaacgtg gctggaacgg gcaacctttc ggtaatcgca
    10561 gcaggtttgg cggattaccc gggatggtca acattgtttc accttccgca tcgagacgcg
    10621 gcaccgcgtt gagcaaaccg atagaataag gatgaacggg ttgataaaag acatcgcgcg
    10681 cgttgccata ttccatcgtg cgcccggcgt acattaccag cactttgtca cagatccccg
    10741 ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt attaaattcc cgtttcagtt
    10801 catttaatag cgtcatgatc tgcgcctgta cggtgacgtc cagcgctgta gtgggttcat
    10861 ccgcaatcag cagcttaggt cgacatagca atgccatcgc aatcataact cgctgacgca
    10921 tgccgccaga aaactcgtgc gggtacattt tcatgcgttt acgtgcttcc ggcattttta
    10981 ccgcatcgag catccgcacc gactcttcaa acgcctcagc tttgctcatg ttcttatgca
    11041 gcatcagcac ttccatcaac tgctcaccga cgcgcatata gggattcaac gaagtcattg
    11101 ggtcctgaaa aatcattgag atttgttcag cgcgcagttt attgagttca tgctctggca
    11161 aattgaggat ttcacgccca ttgaaggttg ccgatccgcc aatacgtccg ttggcagcca
    11221 acaggcccat caacgcaaat gcagtttgcg atttacccga accagactca cctacaatac
    11281 ccagcgtttc tccggcacgt agggaaaaat tcaaatcatt gaccgtcgtg acgtcgccgt
    11341 ccggggtact aaaggtgaca cgcaaatctt tcacgttcag cagtgcgtca gcctgttgtt
    11401 gtgcgagcgg cacagttgca gtttcaatta cgctcatggc tgcactcctt aacgatcttt
    11461 cgggtcgagg gcatcacgca agccatcgcc gataaagttg aaacaaaaca gcgtcaccac
    11521 gaggaatccc gctgggaaca acaataacca tggagagact tccatcgagt tcgcgccatc
    11581 actcagcaat gcaccccagc tgcttaacgg ctcttgcgta cccaacccca ggaagctaag
    11641 gaaagattca aagaggatca tgctgggcac cagaagtgat gcgtagacca ccaccacacc
    11701 gagtacattc ggcacaatgt ggcgaataac aatgcccgat gtcgatacac cgccaacttg
    11761 tgccgcctca ataaactctt tgcgcttcag actcagggtt tgcccacgca caatacgagc
    11821 catatccagc caggaaacca tgcctatcgc cacgaaaatc agcaggatgt tttgaccgaa
    11881 aaaggtcacc agcaaaatga cgaagaacat gaatgggaag gagttgagga tttccagcag
    11941 acgcatcatt accgaatcca ctttaccgcc cagataaccg gaaagcgaac cataaagtgt
    12001 cccgacgacc acagccacca gtgccgcagc aacaccgacc atgagtgaga tacgcccacc
    12061 aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca gtaccaaagt agtgaccgga
    12121 ctccatatcc ggggcgctgg acatcatcgc ccagtcagta tcgtcatagg caaactggga
    12181 aagcatcggt gccaggatta caaataacgc gatcagcacc agcaccatca gactggcaac
    12241 cgccgcacgg ttatgaataa aacgtcgacg tgcgtcctgc cacaagctgc gcccttcgac
    12301 ctccagcttt tcactgaaat tttccagcgt ctcgctgttt ttcttactta acatcatcgc
    12361 gagctccagt atcagtaacg gattttcggg tcgataaccg catatagcac atcgacaatg
    12421 gcattaaaca aaatggttaa agcaccaacc aggatggtca ggcttaacac taaggaatag
    12481 tcacggttca atgcgccatt aacgaacagt tgtccaatcc ccggcaaacc ataaatggtt
    12541 tcgataacca tagaaccggt aataatgccg acaaatgcgg ggcccatata agagagcacg
    12601 ggtaacagag caggttttaa tgcgtgacgt aaaatgatcc gccgcatagg taaccctttc
    12661 gcccgggcag tacgaataaa attggagtgt aatacttcaa tcatagagcc acgggtaata
    12721 cgcgcaatac tggcgatata agcgagtgac aacgccacca tcggcaatat catgaattta
    12781 agcgccccac cattccagcc accgcccggc agccaatgca aaatgatcgc aaatatcatg
    12841 actaataatg gtgcaaccac aaaactgggg ataacaaccc cggtcattgc cagccccatc
    12901 acggtatagt cccatttggt gttttgtttt aatgcggcaa taacgccagc actaacaccc
    12961 agtattaccg caaggaaaaa tgctgcggct cccagtttgg cagaaacggg aaaactggat
    13021 gcaaccaagt cattgaccga ataatcttta tatttaaacg atggaccgaa atcaccgtgc
    13081 gccagttgtt tcaggtagct gaaatactgt gtcatgatcg gatcattaag atgatatttc
    13141 gcttcgatat tggccatcac ttctggcggt aaagtacgtt cgccggtaaa agggcttccc
    13201 ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa taaatagcgt cggaatcgct
    13261 tccagacagc gacgtagaat aaattttaac attgccctac cttctggcct gtgccttctg
    13321 taatgcgata aaaatcagac accgtggagc aggacactcc tgccccacgt attgccatta
    13381 gtgcttcaca atgtacatat tccgggtata ggtattatcc agcggatctt tgccggtata
    13441 gccaccaacc cacggtttca ccagacgcgc attcacgtag taataaacag gaacaatggc
    13501 cgaatcctta tccagctgtt gttctgcttt agtgtacaga gctgtgcgct gcgcctcgtc
    13561 agtcactttc agcgtttccg ccataatgct gtcaaaggcc gggctcttat aatgcgcggt
    13621 attcatcgag ctgttcgaaa gcatggtgtt caggaaggaa gttggttcgt tgtagtcagc
    13681 acaccagcct gcacgggcca catcaaaagt accctggtga cgggtgtcga ggaacgtttt
    13741 ccactcctgg ttaaccagtt tgacgtttac accaatgttt ttcttccaca atgaagaggc
    13801 agcaatcgcc agctttttat gcagatcgga ggtgttatac aacaggttga ttgtcaacgg
    13861 tttgtctgcg gtataacccg cttcagccag cagttttttc gcttcttcgt tacgtttttc
    13921 ctggctccag ccaaaccatt ccggctgagt caattttgcg ccatcagtat acggtggagt
    13981 gtaaccatag gcgggcatgt cgccctgcgc tttcacttta ttaacaatga tatcgcggtc
    14041 catacctagt ttcagtgcgg tacgcacacg cacatcgttg aatggcggtt tctggttgtt
    14101 aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga acttcgtccg ggatctcttt
    14161 tttcagcttc tggaacaatt cgatcggcat gttgttataa gtcatgtcga tttcaccact
    14221 acggtagcgg ttgacatcgg taacttcaga agcaataggc aaataggtta cctggttaat
    14281 aacggttttc gcgttgttcc agtaggtcgg gctgcgttca agaacgattc gttcgtttac
    14341 gacccaatct tttaaggtat aggcaccgtt ggtgacgata ttaccaggct gggtccattt
    14401 ttcgccgaat ttctcgatag cggcttttgg caccggtgaa gttgatgggt gaacaagtaa
    14461 tttatagaag tacggaacgg gttcacttaa ggtgacttct aatgtgtgat catcaatagc
    14521 tttcacaccg agatcggtaa tcggtttttt cccttcaaga atttcatcaa taccggcgat
    14581 atgcccatat tgcagataac tggcatacgg agaagcagtg ttcggatcaa cagaacgttg
    14641 ccagctatac acaaagtctt gtgctgtgac tggcgtacca tcagaccatt tcgcatcttt
    14701 acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc caggattcag cgacgccagg
    14761 tgccggatga ccgtcaagat cgctgaccag taagccttca aacaggtctc ggctgatatt
    14821 agactccgga acaccttcaa ttttgtgcgg atctaatgac tgaacttctg aaccattgtt
    14881 acgtaccagc gtttgttttt ccgccagtgt gacgcctgcg ggtacatcag ctgccagcgc
    14941 gacattccct gccattagcg cagccagaac gccagctgct actaaacttc tcttggtgat
    15001 gttggtcatt gttttttgga ctccctcatt ataattactg gtattaacca gcatgtgtaa
    15061 tcccggattg ggaccctgca caggctcgga gactttctgc tgtcaggtta ctttatcgtt
    15121 acggattgct atcaccgact ttatttattc tggtgattca gacgatcacc ttatgtcgat
    15181 tcgtactgtg tctcccctgt cgagacacat tattctttcg cgacgctctt taattgttaa
    15241 ttgttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
    15301 tactaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagca attctctttt
    15361 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
    15421 tcaaatagag attggctaag catgtctatc ttttctgatt acagtttcag tattgaaatg
    15481 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg cagtggaatt
    15541 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
    15601 tgatggtgag gagaagcagt taagaaaatc cttataacta actgtaattt gagaagaata
    15661 tttttaccta ttccttttgc ttttgataac t
//
LOCUS       DAJTNN010000092        15640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTNN010000092 DAJTNN010000000
VERSION     DAJTNN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.092"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            337..429
                     /locus_tag="OO150_002519"
                     /pseudo
     CDS             337..429
                     /locus_tag="OO150_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            433..612
                     /locus_tag="OO150_002520"
                     /pseudo
     CDS             433..612
                     /locus_tag="OO150_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            946..3225
                     /gene="ydeP"
                     /locus_tag="OO150_002521"
     CDS             946..3225
                     /gene="ydeP"
                     /locus_tag="OO150_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5516620.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHTASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIEHISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3473..3670
                     /gene="safA"
                     /locus_tag="OO150_002522"
     CDS             3473..3670
                     /gene="safA"
                     /locus_tag="OO150_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5516621.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3745..4506
                     /gene="ydeO"
                     /locus_tag="OO150_002523"
     CDS             3745..4506
                     /gene="ydeO"
                     /locus_tag="OO150_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5516622.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4908..6590
                     /locus_tag="OO150_002524"
     CDS             4908..6590
                     /locus_tag="OO150_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5516623.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGTVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYNLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6642..7799
                     /locus_tag="OO150_002525"
     CDS             6642..7799
                     /locus_tag="OO150_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5516624.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7765..7887)
                     /locus_tag="OO150_002526"
     CDS             complement(7765..7887)
                     /locus_tag="OO150_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516625.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8102..9787
                     /locus_tag="OO150_002527"
     CDS             8102..9787
                     /locus_tag="OO150_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR5516626.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            9825..12197
                     /locus_tag="OO150_002528"
     CDS             9825..12197
                     /locus_tag="OO150_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5516627.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVVGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12254..15037
                     /locus_tag="OO150_002529"
     CDS             12254..15037
                     /locus_tag="OO150_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5516628.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKKALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            15399..>15640
                     /locus_tag="OO150_002530"
     CDS             15399..>15640
                     /locus_tag="OO150_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5516629.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         4556 a         3400 c         3425 g         4259 t
ORIGIN      
        1 tggtgacggc agttgaaacc aatgtgttga ggttgcatgg acggttaagc cgagaatacg
       61 gtagtaagtg agatatgggg tttggtcgtt gttaatgagc aaccatgacc atgaccgcca
      121 acgactggat tctcccttgg tttatgttgg tgcgcataat gtatattatg ttaaatcata
      181 tctgagtgat gcgaagtgct tttctggtag tcgttattcg ttcagggttc cagaacttaa
      241 gcctctcctc tttcataatt ggcctgtgca tgacggatat aggctggctg gtatgcagat
      301 ttgccgtttg ccgcatcttc ccagcgttga agactcaagc tggcaaaccg cgtgcaaatg
      361 agcacgccct ctttccatca acacttcagg cagcttgcgg gaatgagtcc actacggtat
      421 cagaaatggg aagtgggtgt ctctctgatg cgtaatggaa aaatactggc tacgggagaa
      481 aatgtctccc tggggacagt aaataaaagc aaagtatctc tgggtttaag cgcaacctat
      541 ggacaaacag gaaacaaggt tgcagccgga accgtccagt cagtcattgg tgtcactttt
      601 atctatgaat aattgtgatt ttgatcttag tctatgcgct ccattcacga aaattgcgcg
      661 gataattatt ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa
      721 gccagagtct tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg
      781 cttaacttat cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt
      841 agtcccctac tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat
      901 cgctattaca aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt
      961 gaatcctacc agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta
     1021 cgtaagcaga tggatatacg ccaggatgtt attgccatgt ttgacatgaa taagccagag
     1081 ggctttgact gtccgggttg tgcatggcca gatcctaagc acactgcgtc attcgacatt
     1141 tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct
     1201 ttctttgctg agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct
     1261 gcgggacgtc tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta
     1321 agctggcaac aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat
     1381 caggttgaat tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt
     1441 tttgcccgtg aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg
     1501 accagtgtgg gtttggcagc gagtatcggc gtgggtaaag ggactgtgtt gctggaagac
     1561 tttgagaagt gcgatttagt catttgcatt gggcataacc ctggtacaaa ccacccccgc
     1621 atgctgactt cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct
     1681 ttacaggaac gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg
     1741 aactctgaga ctcagttggc cagtgcgtac tacaacgtgc gcattggtgg cgatatggcg
     1801 ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt
     1861 cggccctcat tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta
     1921 cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac atatttctgg actaagtcag
     1981 acacaaatcg ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac
     2041 ggaatgggga tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg
     2101 ctgttgatga aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac
     2161 tctaatgtac agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg
     2221 gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt
     2281 gccagcatgc aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac
     2341 tttgcactgg caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg
     2401 gcggtacacg tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat
     2461 attctgccgg ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta
     2521 accgttgagg attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt
     2581 gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccag
     2641 agcgtggtag cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa
     2701 gctgtgttac cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac
     2761 ctgataaatg cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt
     2821 accagcaaag ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg
     2881 acagtacgca gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga
     2941 ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt
     3001 gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca
     3061 cgccgcatgg atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc
     3121 tattttccag aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt
     3181 cctggttata aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt
     3241 cttctgctgt catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct
     3301 cacattgatc aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga
     3361 aactccgctc agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta
     3421 agcatactga ttaacaattt ttaacgttat ccgctaaata aacatatttg aaatgcatgc
     3481 gaccacagtg aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat
     3541 tgtggttgtc ttattactga cccttacgtt gatagccatt ttttcggcaa ttgatcagct
     3601 gggtatttca gaaatgggtc gcattgcaag agatcttaca catttcatta tcaatagttt
     3661 gcaagactga aaataaacag caaattataa atatgaaatg ttaaaaaagt atcgataaaa
     3721 actttattgt tttaaggaga taaaatgtcg ctcgtttgtt ctgttatatt tattcatcat
     3781 gccttcaacg ctaacatttt agataaagat tacgccttct ctgacggcga gatcctgatg
     3841 gtagataacg ctgttcgtac gcattttgaa ccttatgagc ggcattttaa agagatcgga
     3901 tttactgaaa ataccattaa aaaatatcta caatgcacta acatccagac agtgacggtg
     3961 cctgttcctg cgaagttttt acgtgcttca aatgtaccga ctggattgct taatgaaatg
     4021 attgcttatc tcaactcgga agaacgcaat catcataatt tttcagaact tttgcttttt
     4081 tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta cactattaac taacggtgtg
     4141 ctatccgttt ccggaaaagt gagaaatatt gtcaacatga agctggcgca cccatggaag
     4201 ctgaaagata tttgtgactg cctgtacatc agtgaaagcc tgttgaagaa aaaactaaag
     4261 caagagcaaa cgacattctc acagattcta ttagatgcca gaatgcagca cgcaaaaaat
     4321 ttgatacgcg tagaaggttc agtcaataaa attgccgaac aatgtggtta tgccagtact
     4381 tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca gtccgaagag agtttctaag
     4441 gagtaccgtt gtcaaagtca cacgggtatg aatacgggca acacgatgag tgctttagct
     4501 atttgattat ttgctaacga gtagtcaacc acgcacgcta cgtaagaatt atatggggca
     4561 gccattccct gccccgcgtt gtttttaggc gatatattta ttgaaataaa taagtgacat
     4621 ccatcacata tttatgcact tgcataacct gttgcatgat tatttatgtt ctcaattcag
     4681 catcttgtca gtaaaatgca ataatttatt aaatatcaat aaattagttg tttatcggcg
     4741 agaaattact taatagaaca gaaagtaatg tcaacgcttt atggactgtt atttcccttt
     4801 ttttagctaa atctgctatc tctttatgtg actaacttca cttacatcca cttatttctc
     4861 ttcgtaaaat tacttcggaa ttaagtacaa taagaagagg aacatttatg aagtctgcat
     4921 taaagaaaag tgtcgtaagt acctcgatat ctttgatact ggcatctggt atggctgcat
     4981 ttgctgctca tgcggcagat gatgtaaagc tgaaagcaac caaaacaaac gttgctttct
     5041 cagactttac gccgactgaa tacagtacca aaggaaagcc aaatattatc gtactaacca
     5101 tggatgatct tggttatgga caacttcctt ttgataaggg atcttttgac ccaaaaacaa
     5161 tggaaaatcg tgaagttgtc gatacctaca aaatagggat agataaagcc attgaagctg
     5221 cacaaaaatc aacgccgacg ctcctttcat taatggatga aggcgtacgt tttactaacg
     5281 gctatgtggc acacggtgtt tccggcccct cccgtgccgc aataatgacc ggtcgagctc
     5341 ccgcccgctt tggtgtctat tccaataccg atgctcagga tggtattccg ctaacagaaa
     5401 ctttcttgcc tgaattattc cagaatcatg gttattacac tgcagcagta ggtaaatggc
     5461 acttgtcaaa aatcagtaat gtgccggtac cggaagataa acaaacgcgt gactatcatg
     5521 acaacttcac cacattttct gcggaagaat ggcaacctca aaaccgtggc tttgattact
     5581 ttatgggatt ccacgctgca ggaacggcat attacaactc cccttcattg ttcaaaaatc
     5641 gtgaacgtgt ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag gcaattggcg
     5701 ttgttgatcg tgccaaaaca cttgaccagc cttttatgct ttacctggct tataatgctc
     5761 cgcacctgcc aaatgataat cctgcaccgg atcaatatca gaagcaattt aataccggta
     5821 gtcaaacagc agataactac tacgcttccg tttattctgt tgatcagggt gtaaaacgca
     5881 ttctcgaaca actgaagaaa aacggacagt atgataatac tattattctc tttacctccg
     5941 ataatggtac ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa ggctataaga
     6001 gtcagaccta tcctggcggt actcacaccc caatgtttat gtggtggaaa ggaaaacttc
     6061 aacccggtaa ttatgacaag ctgatttccg caatggattt ctacccgaca gctcttgatg
     6121 cagccgatat cagcattcca aaagacctta agctggatgg cgtttccttg ctgccctggt
     6181 tgcaagataa gaaacaaggc gagccacata aaaatctgac ctggataacc tcttattctc
     6241 actggtttga cgaggaaaat attccattct gggataatta ccacaaattt gttcgccatc
     6301 agtcagacga ttacccgcat aaccccaaca ctgaggactt aagccaattc tcttatacgg
     6361 tgagaaataa cgattattcg cttgtctata cagtagaaaa caatcagtta ggtctctaca
     6421 atctgacgga tctacagcaa aaagataacc ttgccgccgc caatccgcag gtcgttaaag
     6481 agatgcaagg cgtggtaaga gagtttatcg acagcagcca gccaccgctt agcgaggtaa
     6541 atcaggagaa gtttaacaat atcaagaaag cactaagcga agcgaaataa ctaaaccttc
     6601 atgcggcgga attttccgcc gccttattga gcgagatagc gatgcacgtt acagccaagc
     6661 cctccagttt tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc
     6721 agtttactca tgaaaaatgg atggatgaca gcactctgaa agagttcatc aaacaatata
     6781 tcgcagcgtc tggcaatcag gtctatttta cctggcaagg cggtgaaccc actctggctg
     6841 gcctggattt tttccgtaaa gttattcact atcaacaacg ctatgcaggc caaaaacgta
     6901 tttttaatgc attacaaacg aatggcattt tattgaataa tgaatggtgt tcctttctca
     6961 aagaacatga atttctggtt ggtatctcga tcgatggccc ccaggagtta catgaccgtt
     7021 acagacgcag taattcaggt aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc
     7081 tgaaatcata tcaagttgag tttaatacgt taaccgtcat taataacgtt aatgtccatt
     7141 accctcttga ggtttatcat tttttaaaat ctatcggcag taaacatatg caatttatcg
     7201 aattgctcga aaccgggacg ccgaatattg atttcagtgg tcatagtgag aacacattcc
     7261 gtatcattga tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta
     7321 tgcaatgggt taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa agctttgtca
     7381 gccgtttttt ggggaatggg catacaagtt gtattttcca ggagtcctgc aaggataatc
     7441 tggttgttga aagtaatgga gacatttacg aatgcgacca ttttgtctat ccacagtaca
     7501 aaattggaaa cattaataaa tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc
     7561 aaaaaaaacg gatttcagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg
     7621 gttgtcctaa gcatcgtatt actaaagtaa acaatgagac tgtttcttat ttttgcgaag
     7681 gttataaaat ccttttttca accatggtac cttatatgaa cgccatggtt gagttagcta
     7741 agaacagagt accgctttac cacattatgg atgttgcaaa acaaatggag aataattaat
     7801 aacgctctct tttaactcca ttcttagcct ccttcttatg accttaacaa ggcacccgac
     7861 ataaattcct gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc ctggcttacg
     7921 tggtatgcgt cagggcatta gcaataccct tgattatcaa tagcataacc ataaccataa
     7981 ccataaccac tgaaaattaa aaatttctat atatttcata ttgttaaatg atacttaaat
     8041 aaatttcatc agtaaatcat tgccatagat aatcattgga tggcacaatc aattttcgct
     8101 aatgataacc attcccatta cgttttgtat gttgatagca aagtatctct gtctgctgaa
     8161 gcccttttgg ctgcgtaaga ataataaaac ttcagtgttg ttaatcatta ttatcctcgc
     8221 aatgatcctt ggcgtggtta aaattcaggt ctggttaaac gactggaata atgatttttt
     8281 caatgcgcta agccagaaag aaaccgataa gctttggcag cttgttcttt ggttccctgc
     8341 cctgctgggg atctttgtat taatatccgt aaacaaaaca tggttaatta aactactgac
     8401 tattcgctgg cgcgaatggt taacagatta ctaccttaat cgctggtttg cagataaaaa
     8461 ctactacttc acgcagatct acggcgagca taaaaataca gataaccctg accaacgtat
     8521 cgctgaagac attctcttac tgatcagcaa aacgctcagt ctttcatttg gcttcatcca
     8581 gtcacttagc atgctgatca cctttaccgt tattctttgg caaagcgcgg gtacgctctc
     8641 attcactgtt ggtggaacag aatggaatat ccaggggtat atggtctata ccgtcgtgct
     8701 cattgtgatc ggtggaactt tatttaccca taaggttggt aagcgtattc gtccgcttaa
     8761 tgtggaaaaa caacgtagcg aagcgacatt tcgaactaat cttgtgcagc ataataagca
     8821 ggcagaactg attgcgctaa gcaatgctga gtctctacaa cgccaggagt tgcgcgacaa
     8881 ttttcatacc attaaagaga actggcatcg tttaatgaat cgtcagcggt ggcttgatta
     8941 ctggcaaaat atttattcgc gctcgcttag cgttcttccc tactttctgt tattgccgca
     9001 gtttattagc gggcagatca atctgggcgg actgatgaaa tcgcgccagg catttatgct
     9061 agtatcgaac aatttaagct ggtttattta taaatatgac gaacttgctg aactggctgc
     9121 ggttatcgat cgcttgtatg agttccatca actcactgaa cagcgcccta cgaataagcc
     9181 taaaaattgc caacatgcgg tacaagtggc taatgcgagt attcgtacgc ctgataataa
     9241 gatcatatta gagaacttga actttcatgt ttcgccaggc aaatggctat tactgaaagg
     9301 ctactctggt gcgggaaaaa ccacactgct taaaacatta tcccactgct ggccgtggtt
     9361 taaaggtgat attttttctc ctgctgacag ttggtatgtg tcacaaacac cgttaatcaa
     9421 aaccggctta ctgaaagaga ttatttgtaa agcacttccc ctgcccgtag acgataaatc
     9481 gttgagcgaa gtactgcatc aggttggtct tgggaaattg gctgcgcgta ttcatgacca
     9541 cgatcgctgg ggagatattc tttccagcgg cgaaaaacaa cgtatcgccc tggcacgatt
     9601 aattttacga cgtccgaaat ggatatttct tgacgaaact acctctcatc ttgaggaaca
     9661 agaggctatc cgcttactgc gtttagtgcg tgaaaaacta cccacaagcg gcgtcattat
     9721 ggttacacat caacccggtg tctggaacct ggccgatgat atttgtgaca ttagcgcggt
     9781 tttataaaaa caataaaagc cctgctgtac ggagatataa ataaatgaag cgagttctta
     9841 ttcctggcgt cattttatgt ggcgctgatg tggcgcaggc cgtcgatgac aaaaacatgt
     9901 acatgcattt ttttgaagag atgacggtct atgctcctgt ccctgtaccc gtaaacggca
     9961 acacgcatta caccagtgaa agcatcgagc gtttaccgac cgggaatggc aatatcagcg
    10021 atctgctgag aaccaaccct gcggtacgca tggattcaac gcaaagtacc tcgttgaacc
    10081 agggagatat tcgccctgag aaaatctcta ttcacggtgc gtcgccctac cagaatgcct
    10141 atttgattga cggtattagt gcaactaata acctgaaccc agcgaatgag tccgatgcca
    10201 gtagtgcaac caatattagc gggatgtcac aggggtatta tcttgatgtc agcttactgg
    10261 acaatgtgac gctttatgac agttttgtgc cggttgaatt tggtcgcttc aatggcgggg
    10321 taattgatgc aaagatcaaa cgcttcaacg ctgatgatag caaggtgaaa ttgggttatc
    10381 gcactacgcg ttcggactgg ttaacatcgc atatcgatga gaataacaag agcgcattta
    10441 atcaaggttc ttcaggaagt acttatttct ctccagattt taaaaagaac ttttatacct
    10501 tgtcgtttaa tcaggagctg gctgataact ttggcgttac cgccggttta tcgcgccgcc
    10561 agtctgatat cacccgcgcg gattatgttt cgaatgacgg cattgtcgtc ggtcgggcac
    10621 agtataaaaa cgttatcgat actgcattga gcaaatttac ctggtttgcc agcgaccgct
    10681 ttacccacga tttaacctta aaatataccg gctccagccg tgattataat accagcacct
    10741 tcccgcagtc tgatcgcgaa atgggtaata aatcctatgg tcttgcatgg gatatggata
    10801 cgcaactcgc atgggccaaa ctacgtacca ccgttggttg ggatcatatt agtgattata
    10861 cccgtcacga tcatgacatc tggtacaccg aactttcatg tacatatggt gatattacag
    10921 ggcgttgtac ccgtggcgga ttaggacaca tttcccaggc tgtagataat tacaccttca
    10981 aaacacgcct ggactggcaa aaattcgccg tgggtgatgt ttcgcatcaa ccctactccg
    11041 gcgcggaata catctattcc gatgcatgga ctgaacgcca taaccagtct gaatcctatg
    11101 tgattaatgc tgcaggaaag aaaactaacc ataccattta ccataaaggt aaaggcagcc
    11161 taggaattga caactacacg ctgtatatgg cggatcacat tagctggcgg aatgtgtcgt
    11221 taatgcccgg tgtgcgttat gactatgaca actatctgtc aaaccacaat atctccccgc
    11281 gctttatgac ggaatgggat atttttgctg atcaaacctc aatgattacc gccggttata
    11341 accgttacta tggcgggaat attcttgata tgggattacg tgatatccgc aatagctgga
    11401 cggaatcggt atcaggtaat aaaaccctga cgcgttatca gaatttgaaa acgccttata
    11461 acgatgaact ggcaatggga ttgcagcaaa aaatcggtaa gaacgttatt gcacgcgcaa
    11521 actatgttta ccgtgaagcg catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga
    11581 ctaaaaccac cattactgaa tataacaacg atggcaaaac caaaacgcat tcgtttaacc
    11641 tcagttttga actggccgaa cccctgcata tccgccaggt agatattaac ccgcaaattg
    11701 tctttagcta tatcaagagc aagggcaact tgtcgttaaa caatggttat gaggagagca
    11761 ataccggtga taaccaggtg gtttataacg gtaatctggt ctcttacgat agcgttccag
    11821 tggcagattt taataaccca ttaaagatct ccttaaacat ggatttcacg catcaaccga
    11881 gcgggttagt gtgggcgaat acgctggcgt ggcaagaagc gcgtaaagct cgcattatcc
    11941 ttggtaagac aaatgcgcaa tacatcagcg aatattcaga ttacaagcag tatgttgacg
    12001 aaaaactgga tagcagcctg acctgggaca cccgcttgtc ctggacgcca caatttctga
    12061 aacaacaaaa cctgacgatc agtgccgata ttctcaatgt actggatagc aaaaccgctg
    12121 ttgatacaac gaataccggt gtggcgacct acgccagtgg ccgtactttc tggcttgatg
    12181 tcagcatgaa attttaatag ttcggatttt acttaaccgg gtaaattcgc ccggttttcg
    12241 catggagata attatgagaa acctctgttt cttactgacg ttagtggcaa ctctgttgct
    12301 ccccgggcgg ctgattgccg ccgccttacc gcaggatgaa aagttaatta ccgggcaact
    12361 ggacaatggc ttgcgatata tgatttatcc gcatgctcaa ccaaaggatc aggtaaattt
    12421 atggctgcaa attcataccg gttcattgca ggaagaagac aatgagcgcg gcgtggctca
    12481 ttttgtagaa catatgatgt ttaacggcac aaaaacatgg ccgggtaata aagtcatcga
    12541 aacatttgag tcaatgggcc tgcgttttgg tcgcgatgtt aatgcctata ccagctatga
    12601 cgaaacggtg tatcaggtga gtttgccgac tacgcagaaa caaaatctgc aacaagtgat
    12661 ggcaatcttc agtgaatgga gtaatgccgc aacctttgaa aaactcgaag tagacgctga
    12721 acgtggcgta attactgagg aatggcgtgc ccatcaggat gcgaaatggc gcacctctca
    12781 ggcgcgccgc cctttcctgc tggctaatac ccgtaattta gaccgtgaac ctatcggcct
    12841 gatggatact gtcgccacgg tcacaccggc acaattgcgc caattttatc aacgctggta
    12901 tcaaccaaat aatatgacct ttatcgtggt cggcgatatc gacagtaaaa aagcgctggc
    12961 gctgataaag gataatttaa gtaagcttcc ggctaacaaa gcagctgaaa atcgcgtctg
    13021 gccgacaaaa gccgaaaacc acctgcgctt taatatcatt aatgataaag aaaaccgggt
    13081 gaacggcatc gcactctatt atcgcctgcc aatggtacaa gtgaacgatg agcaaagctt
    13141 tatcgaacaa gctgaatgga gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg
    13201 catacagtcg ggcgagttga agactatttc tggcggcact gcgcgcagcg ttaaaattgc
    13261 acccgattat cagtcgctgt ttttccgtgt aaatgcacga gacgataata tgcaggatgc
    13321 tgcgaatgca ttaatggcag agttggcaac cattgatcag catggttttt ctgctgaaga
    13381 actcgatgat gtcaaatcta cccgcctcac ctggctgaaa aatgcggttg atcagcaagc
    13441 tgaacgtgat ttacgtatgc tgaccagtcg cctggcatcc agctcattaa ataatacgcc
    13501 gttcttgtcg ccggaagaga catatcaact ttcgaaacgt ctgtggcagc aaattaccgt
    13561 gcaaagtctg gcggaaaaat ggcagcagtt aagaaagaac caggacgcat tttgggagca
    13621 aatggtaaac aatgagcttg ccgccaaaaa agcattgtct cctgcggcta tcctggcgct
    13681 ggaaaaagag tacgccaaca aaaagctggc ggcttacatc ttcccaggca gaaatttatc
    13741 gttaacagta gacgctgacc cacaggcgga aattagcagc aaagaaacgc tggctgagaa
    13801 tctgacatca ttaacacttt ccaatggtgc cagggttatt ctggcaaaat ccgcgggtga
    13861 agagcaaaag ctacaaatta ctgccgtatc gaataaaggc gatttaagtt tccctgcgca
    13921 gcaaaaatca cttatcgcgc tggcaaataa agcagtaagc ggaagcggcg ttggtgagct
    13981 ctcctcttcc agcctgaaac gctggagtgc ggaaaattcg gtaaccatga gcagtaaagt
    14041 cagtggcatg aatacgttgc tctccgttag cgcgcggact aataaccctg aacctggttt
    14101 tcagttgatt aaccagcgaa tcacccacag cacgattaac gacaatattt gggcatcgct
    14161 acaaaatgct caaattcagg cgttgaaaac gctcgaccag cgtccagcgg agaaattcgc
    14221 ccagcagatg tatgagacgc gctatgctga tgaccgcacg aaattactgc aagaaaatca
    14281 gattgtacag tttactgccg cagatgcgct ggctgccgat cgccagttgt tttcatctcc
    14341 agcggatatc acgtttgtca ttgtcggtaa tgtctcagaa gacaaactcg tggcgttaat
    14401 tacgcgttac ttaggatcaa tcaaacactc tgattcgcca ttagccgcag gtaaaccatt
    14461 aactcgcgcg acggacaacg catcggttac tgtaaaagaa caaaatgaac ctgtggcaca
    14521 ggtttcacag tggaagcgtt atgattcccg gacacctgtt aatctggcga cgcgtatggc
    14581 gctcgatgct tttaacgtcg cactggcaaa agatctacgt gttaatattc gtgaacaggc
    14641 atctggagca tacagcgttt cttctcgcct ctcggttgat cctcaggcca aagatatcag
    14701 tcatttgctg gcttttactt gtcaaccaga acgacatgat gaactgttaa cgttggcgaa
    14761 tgaagtgatg gttaagcgtc tggctaaagg gatcagtgag caagaactga atgaatacca
    14821 gcaaaacgtt cagcgcagcc tcgatatcca acagcgtagc gttcaacaat tagcgaacac
    14881 tattgtaaat agtcttattc aatatgacga tcctgcagca tggactgagc aggagcaatt
    14941 gttgaaacaa atgacggtag agaatgttaa cactgccgtt aaacaatatc tttctcatcc
    15001 ggtaaatact tataccggag tattattgcc aaaataataa cagccccgtc aacacatcgt
    15061 tggcggggat tttagcaata ttcgctattt ttatgtaata attttataaa tgcgttcaaa
    15121 ataataatca agtactaata gtgatatttt aaggtctgat ttttacgtga taattcagga
    15181 gacacagaat gcgcataaaa ataacagcat aaaacacctt accaccaccc aagaatttca
    15241 tattgtattg tttttcaatg aaaaaacatt attcgcgtaa tatctcacga taaataacat
    15301 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
    15361 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
    15421 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
    15481 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcattccag attatcaatg
    15541 acgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
    15601 acgacgaaaa tgtccacaaa ttgatggatt tatccattaa
//
LOCUS       DAJTNN010000093        15301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTNN010000093 DAJTNN010000000
VERSION     DAJTNN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.093"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..293
                     /locus_tag="OO150_002531"
     CDS             <1..293
                     /locus_tag="OO150_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5516630.1"
                     /translation="YRRAKTITLVADNYIIHKGRKVERWLEENPKFRLLFLPTYSPWL
                     NPIEQLWLSLHKTITRNHQCRYMWQILKQVTQFMNAALPFPGNQHGMAKVER"
     gene            507..1655
                     /locus_tag="OO150_002532"
     CDS             507..1655
                     /locus_tag="OO150_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5516631.1"
                     /translation="MFEIDSLLLITSVIILSLLAVKLFDEISPIQLVDHGRNNQIDGM
                     RGFLAIFVLIHHAAIWHGYLLTGVWKTPSSNLLTNLGQVGVSFFFMITGYLFFSKIRS
                     SDQDWVRLYISRFLRLTPMFIVSLCLVLLVIGFKSRWSVHVSPASLTVSLMRWAPFTA
                     LGMPNINGVKDSFTINAAVTWTLVYEWFFYFSLPVIAALFKRRVSIHMIIISVLVLVI
                     FLCYPSKKIHVISFLFGLLASYSNNYVHRVAKAKITPIMIIALLSYEMIYFPTTYAPL
                     PLIICGVCFILIASGCDFYGILRLNLTRKLGETTYSIYLLHGVFLYCVMTWVIPSNYS
                     NYFFIMLISITAFCVTLLSCITFKLIELPFINITKQTAMKIRGIIKNT"
     gene            complement(1935..2546)
                     /locus_tag="OO150_002533"
     CDS             complement(1935..2546)
                     /locus_tag="OO150_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5516632.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIVPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTAMASINLIQLKLQAG
                     RNLTQAETSRLNTVLDYIDAVTATDTSTAPDVIWPELLDAHNM"
     gene            complement(2546..3328)
                     /locus_tag="OO150_002534"
     CDS             complement(2546..3328)
                     /locus_tag="OO150_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR5516633.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEG
                     SALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDD
                     GRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLESFTGTTILKQITPLSPSIDF
                     DNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
     gene            complement(3332..3916)
                     /locus_tag="OO150_002535"
     CDS             complement(3332..3916)
                     /locus_tag="OO150_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR5516634.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMQELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRFWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(3907..4965)
                     /locus_tag="OO150_002536"
     CDS             complement(3907..4965)
                     /locus_tag="OO150_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5516635.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATVASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTTTDDAISSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NITIAKNELAVLGAISWT"
     gene            complement(4952..5377)
                     /locus_tag="OO150_002537"
     CDS             complement(4952..5377)
                     /locus_tag="OO150_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR5516636.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLRRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(5377..5925)
                     /locus_tag="OO150_002538"
     CDS             complement(5377..5925)
                     /locus_tag="OO150_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR5516637.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(5925..7004)
                     /locus_tag="OO150_002539"
     CDS             complement(5925..7004)
                     /locus_tag="OO150_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR5516638.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARKRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(7001..8329)
                     /locus_tag="OO150_002540"
     CDS             complement(7001..8329)
                     /locus_tag="OO150_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR5516639.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(8390..10225)
                     /locus_tag="OO150_002541"
     CDS             complement(8390..10225)
                     /locus_tag="OO150_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5516640.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYQAVDPAQSNNASGATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(10367..10636)
                     /locus_tag="OO150_002542"
     CDS             complement(10367..10636)
                     /locus_tag="OO150_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5516641.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(10636..10992)
                     /locus_tag="OO150_002543"
     CDS             complement(10636..10992)
                     /locus_tag="OO150_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5516642.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNKTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(10992..12488)
                     /locus_tag="OO150_002544"
                     /pseudo
     CDS             complement(10992..12488)
                     /locus_tag="OO150_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
     gene            complement(12472..12642)
                     /locus_tag="OO150_002545"
     CDS             complement(12472..12642)
                     /locus_tag="OO150_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5516643.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(12669..13211)
                     /locus_tag="OO150_002546"
     CDS             complement(12669..13211)
                     /locus_tag="OO150_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516644.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHT"
     gene            complement(13208..13714)
                     /locus_tag="OO150_002547"
     CDS             complement(13208..13714)
                     /locus_tag="OO150_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516645.1"
                     /translation="MATPFFHVDVQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKKRAGFMTRIAPNQRNGKGNRMISGDFYP
                     AFLFFGVRGGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(13689..14099)
                     /locus_tag="OO150_002548"
     CDS             complement(13689..14099)
                     /locus_tag="OO150_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5516646.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCDDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGSNGDSLFSR"
     gene            complement(14096..14419)
                     /locus_tag="OO150_002549"
     CDS             complement(14096..14419)
                     /locus_tag="OO150_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5516647.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            complement(14422..14622)
                     /locus_tag="OO150_002550"
     CDS             complement(14422..14622)
                     /locus_tag="OO150_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516648.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            complement(14671..>15301)
                     /locus_tag="OO150_002551"
     CDS             complement(14671..>15301)
                     /locus_tag="OO150_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR5516649.1"
                     /translation="PQATQKMLDDAFFNVEAWINSELATEFAEQEEIAFTTGDGTKKP
                     KGFLANESTDETDKVRAFGKLQHIVSGDATAVTADAIIKLIYTLRKAHRTGAKFMMNN
                     NSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQMPDIAADAKAIAFGNFKR
                     GYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLLKIAVA"
BASE COUNT         3497 a         4360 c         3680 g         3764 t
ORIGIN      
        1 gatatcggcg ggcgaaaacc atcacactgg tggcggataa ctacatcatc cataaaggcc
       61 gtaaagtgga gcgatggctg gaagaaaacc cgaagttccg gttgctgttc ctgccgacgt
      121 attcaccgtg gctgaatccg atagagcaac tttggttatc gttacacaag accataacgc
      181 gaaatcatca gtgccggtat atgtggcaga tactgaaaca ggtaacgcag tttatgaatg
      241 ctgctttacc gtttccgggt aatcaacacg gtatggccaa agtggagcgg taatatgcga
      301 agctatttag attcagtttg aatctcagcc ctgacattca ttctttaccg acgttccatt
      361 agcccaaatc tgagtatata acctcaattc aactgactaa atttaacaca atatcaatga
      421 caaatgtctt taccattggt acatacaatt gcaaaatcac catctctaag caataagact
      481 acgcatatta aagtgagtta tattaaatgt ttgaaattga tagcctatta ttaataacat
      541 ccgtgataat cttgtcatta ttggctgtaa aattatttga tgaaatttct cctatacaac
      601 ttgtcgatca tggaagaaat aatcagatag atggaatgag aggattctta gccatcttcg
      661 tacttattca tcatgccgct atttggcatg gctacttatt aaccggggta tggaaaactc
      721 cgtcatcaaa cttattaaca aatttaggcc aagttggtgt ctcattcttt ttcatgatca
      781 ccggatatct atttttttcg aagattcgct caagtgacca ggattgggta agactatata
      841 tatcgagatt tctgcgatta actcctatgt ttattgtaag tttatgttta gttcttcttg
      901 ttataggttt taaatcaaga tggagcgtac atgtctcccc tgcctctctt actgtgtcac
      961 tcatgagatg ggcaccattc accgcattag ggatgccaaa tattaacggt gttaaggact
     1021 cattcactat caatgcagct gtaacatgga ccctcgttta cgaatggttt ttttattttt
     1081 cattaccggt tatagctgca ctcttcaaaa gaagagtcag catccacatg attataataa
     1141 gtgttttagt tctagtgatt ttcttatgtt acccatcaaa aaaaatacat gttatttcat
     1201 ttttatttgg actgttagcc tcttattcaa ataattatgt acatagagtt gctaaagcta
     1261 aaataacgcc tattatgata attgcattac taagttacga aatgatatat tttcctacaa
     1321 cctacgctcc actccctctc ataatttgcg gtgtttgctt tatacttatc gcatcaggct
     1381 gtgattttta tggtatattg agattaaatt taacaagaaa acttggtgaa acaacatata
     1441 gtatttatct tttacatgga gtattccttt attgtgtaat gacatgggtc atccctagta
     1501 attattctaa ttattttttt attatgttaa taagcattac tgctttttgc gtcacacttt
     1561 tatcatgcat aacatttaaa ttaattgaac tacctttcat taatataaca aaacaaaccg
     1621 ctatgaaaat caggggcata ataaaaaaca cttagtaatc aattgtcacc taatcaagag
     1681 gtggtttata gataacaata aagaaccttc gccatttccg gaggttcttt tatatcctta
     1741 tggtaaatat aaattataaa tattattttt atggtgttcc actatgaaca gggaagctaa
     1801 ataatgatta caaaaatatg taacttcagg caatgcaggg gctatgttat tgccttaaca
     1861 ccataatgag taatattgtg tcccgaggat aaaagtactg ttaattctta ttgatgcttc
     1921 aatagcactc aaaatcacat attatgtgca tccaacaact caggccagat gacatccggc
     1981 gcggtgctgg tatctgttgc cgtcaccgcg tcaatgtaat ccagcaccgt gttaagtcgg
     2041 ctggtttctg cctgcgtcag attccgtccg gcctgtaatt ttagttgaat cagattgatg
     2101 gaagccattg cagtatcaat cagcgactgg cgttgtgctt ctgctgcatc gactgcggcg
     2161 ctatgttgtg cctcggtatc cgtcacccat ttctcaccat cccatttatc gtatggtgtt
     2221 aacgggacga tagtggttgt attatcaggg taatcacccg gagctgtgat ttcttttgat
     2281 tctcctgttt cggtgctaaa gacgatttca ccgcgatggt ctggcacata ttcccatgag
     2341 ttaaaatctg cagaacggca gattgcataa ccagctttgt atgcgcctgg agcatctaaa
     2401 caggaatatg ccggaatgcc gacaccaata gcaagatatt cagttgaagc tgaaatatac
     2461 tcccgactca tgacatcaaa gttataaaca gtaatttctc ctgccatagt ggcaattaat
     2521 tcactgttta attctgcgtt attcattatg cagccctcac aatatagtta aaagcgacat
     2581 ttcgtgggcg tgtttcattc gcacctgcag gtttagtcgt tgccgcaaca acaccaccct
     2641 ctgtaatagc cgggttggga attgggtaat tatcgaagtc aatggacgga gacaggggcg
     2701 tgatttgttt taaaatcgtg gtgcctgtaa aactttccag agtctggtcg ttagttcgtg
     2761 aagtccagat tccccaatag tggttatgac tgacaatagc accgtcctga agcgttaata
     2821 atccccttcc gttatccact ccacgcccgt catcccagcc ccgaataaac tcaccgcgta
     2881 aatcaggtaa tttatttgtc ggataagcct ttgccagttc cgggtattct tcagcagaaa
     2941 aagctgctcc gttgcatttc agccagcctg ttggcggagt ggctgaaggc cacggaacag
     3001 gcacaccaac gggtaatgct gagccttctc ccaaaccaag gttttcgaga gctgttttca
     3061 ccgtgccatc cgatttgata tcgccaaacg gattcttgcg gcttaacagc agcgcacgaa
     3121 gcgcggtaag cagctggtca tgccgcccct tctccaggct ggcaccggat gcctccacaa
     3181 cgctgcaaag ctcctcctgc aacatgtcaa agtagtcatc atccagatcg gtggcaggcg
     3241 tgccggtctg ggggttacca cgggtaaaac cgttcttacc cgcgccgaac ttatccttct
     3301 gcgcggtttt cgtgtctata cgatgcatgg attactccgg atatttaaaa atcacatagg
     3361 tatgcgacgg gcagagtttg ttaagaacac actcgacaac ggtgtccccc cagaagcgca
     3421 gcgcggaatc acagggatcg ccacatgtca tccaggtggt gttggtggcg gctggcatgt
     3481 tgacctgcca gtaataccgc cattccggcg cattcaccgc gtcagtacag gcagatgagc
     3541 aggtgaacgt gcttttgtcg tatcgcgtga tggtggcgtc tggtctgccc aaggcagcaa
     3601 gctgtgcaag gtaaaaatcc tcattgatgc cgcccgccag gttaaccttc gcatccagcc
     3661 gttgctgacg ctggcgaagg gtctgcgtcc ctgccggaat acattcatcc ggcaggccgc
     3721 acagacgctc ccagcggttt atcagttcgg tggtggtgcg cggatccagc tcctgcatca
     3781 gggcatccgc acgctgatga acacgggtta atgacggtgc cgcaccggca atcgccggat
     3841 cgctggctga ccacgccgga ccgggcggca acagtgctga caacagacgg atgtaatcat
     3901 cgtttgtcac gtccatgaaa tcgcccccag aaccgccagt tcatttttcg caatggtgat
     3961 attgtccgcc ggtgcaagca actgatggct gtattccccg ttcgcaccgg aaatcgcttc
     4021 actgatacgt gacaccttca gttctccctg cggataacca tcacgcagca ggaacgaacg
     4081 caactccgcg gtgatggcag cccgtatttc cggtgtgtcc ggcgtcacac ggatatgaaa
     4141 atccaccgta tgtgccaccg gcctgaacac atacaaatca gagcctgcca ccggggccag
     4201 tggcccgata tgttgtcttg ccgccgtttc cgttgattct tccggaatgg gattaatcag
     4261 gtcactgctg gcaatcatca caccgacagt ccccgttccc atccagtgtc ggtatgtcca
     4321 tgcgcgggta atgccaggca cttctttagc ccagacgaca tagtccccgt cagccccgcc
     4381 ctgcggcgtc cagtaatacc gctcaatgac gcgggcgcgc cacgtttcca gctcttcagt
     4441 atcaaatccg cctgtcaggg tgtcagccac accggaagac ggcagaccat tcaccggcgt
     4501 gaccaggatt aatgccgtac cgtcgtcagc gttaccgacc gcacctgcac ttgagcaggc
     4561 gatcggcacg cgcaggacac caccggagct gattgcatcg tcagttgtcg tgtactgcac
     4621 caggtcatcg cgctgaataa cactcccggc agtcaccttc aggccatcgc tgacaccttc
     4681 ccagcgcata tacccgctgg caaccgtggc ccccttgcgc ggacaccgtt tcatcgcagc
     4741 atgtcgcgcc agccaggact catcgcacag gtcaggcagc atattcattg ccagataatc
     4801 gatgtaaccg tagaccgtat gcagcgccgc cgcatacacc tttgcccgca cgtcttcatc
     4861 catgcgccgg agcgtgtcgc tgacgtccag cctggcgaat aaatcgttac ggagcatact
     4921 gatattttct gccagcgtcg ggcgctgaaa ttcactgtcc gccatgcgtt atcgcactcc
     4981 acagatcatc aaaagaaatc attactggtc catcacgacg cctgagggtg atactgttac
     5041 ccagctcatt aatcccggtg cggcggatat ccagatcaat acgggacacc acgccgtcat
     5101 cagtcatcca ttgcaggcat tcgcggatat atccccttac cgtctgcacc agctgattgg
     5161 tcagtttgct gcgctgaagc aaccacagtc gggagccgta acggtcattc tgtaccgcag
     5221 gccaggtatc cccccaccat cccatcggga cgtcggcatt gtcatcaggc tccgcccgcc
     5281 gccaggtgaa cagggaaatc accacggcgc gggtcagcgg atccagcggt gcgctggcgc
     5341 aggtgcgttt accgttcacc gtcagccaca gttccatcat gcctccatcg ctttatcagg
     5401 tttgtcggtg ttactgccct gaccgttctc tctgtgacga tgcccgttat aggcaagccg
     5461 catcgctgac atggtggtgc cgccggagtc gcacaggtct ttcacctgtc cggtcacttc
     5521 cagatccatt tcaaaacgtg cttcaggtgc attgcgaaac gtgatcgttt tccctgcacc
     5581 gtccaccacg atcccctccc gggtcagcgt cacagactgc ccctgatcgt catagacagc
     5641 cacctcaccc gtttgcagcc ctttcaggcg gtagcgccgg tccgacaccg taacaaccac
     5701 cgcatgagaa cggtcgccat ccggaaacaa caccaccgct tccgcaccgc tgtttgccct
     5761 tgcggtaaaa ccgtagggtt caagatgttc aaccccggct ttgggttcac cggcaatcag
     5821 ggacacatcc acggtctgac atttcgtggc ggcactgatg cttttcacca ctgcccgccc
     5881 aatcaggccg agaagttgtc gctgcatggc ttcaatcgtc ctcatcagaa cgggtcctcc
     5941 tgtactctgg cttttttctt tttccgcgcg ccgggggctt cgggttcagg cagataagcg
     6001 tcaggcgggc cgacacggat ttccgtcagg gtgccgttct ggtcctgagt aaacgtgact
     6061 tccgagacaa gcagttcggt attgtcgaaa ccacagaccg gatcgaagac aatcacccgc
     6121 tggttgggct gccacagcgt accgttaccc tgtcgccagc cctgcaccac ataggtggtt
     6181 tcatccgtcc gcgccgcccg ttttcgggct tcaaagtcag cacgcgcaat acagcctgcc
     6241 cccgtggcct gccctgtctg cctgatatac atcggacggt aacgggcaat aaatgcgtcc
     6301 tctgtgcggg cccgcagcgc ggtggtggtg gcctcaccga aatcatcgtc gtttccggca
     6361 cgctgccccg ccacctggta aacagaaaac cgctcccgga tactcttctc cgtatcacag
     6421 gaaaggatgt tttccccaag taccagcgcg gtatgtgccc gcgttgagcc aataccgcca
     6481 atcaccagcc tgccgtgcgg gtcgtcgtaa gccagcgcct gctgctgacc gagtattttg
     6541 ttgattacct caatcaccgt ttcaccgtga tcaggctgaa catcaggaat aacacccgac
     6601 ggcgcaccgc tgttcaccac ctcaatgccg aaaggcgcag caagcgcctg cgcaatctgc
     6661 accagcgagc gtccgttaaa ctgtgtcggt tcggctgcac agtcaatcag gtcagccgtc
     6721 agactacgtc cggcaatacc ggtgctgacc gaacgggcat cgtaacgaac gggagtcgcc
     6781 tccacccagc cggtgatcac cagctcatca ccaatcagca cttccacttt tgaaccgttt
     6841 ttaatgcgcg gctgaagcgt ggtgataccc tcatctcccg gccattggcg agtgatctcc
     6901 acactgaaat cccgcgccag ccgttcaata ccggcaccga tgcgcaccga tgtccagcca
     6961 ttccactccc ggccatttac ccgtagcgtg acattgtcgt tcattgcact ggcaccttca
     7021 gagggatcac cggcacaaag ccgggatgcg taatggcatt acgccgaata atgtccgcgt
     7081 cacgcgccgc gttatcaaac caggtcgccg ccagcaccag cgcgggtaaa acctcatccg
     7141 gcgtgcgctg aatgatccgt gcagactgtt caaggcgcgt gttgatatcc gcattcagat
     7201 ctgctttcac ccggcgcagc gccagaaaca gcgcatcact ggttgtacgg gacaactcct
     7261 tatcaattgc cgtattcagt gtgtcgcgaa tgtcggtcag ttcttcccac gttggcaggt
     7321 caaccgtgtt tttcaccgcc ggtgcattgt tcagtgccgg atgcgtgaca gaaggccagc
     7381 cggtgctctg cgcgggtgtt gttgactgcc ctactgtggc attctgcatc accgcggaag
     7441 ttgtgggcgc aggcaatcgt gtgacggcat acgccgcttc gctgattgcg gtcgtacgaa
     7501 gggtgctggc aaccacgtta cgctgctgcg tcgccgtggc ggtagtttta ctgtccgttt
     7561 tccagacgcc gcgcggttgc agatcgctgc cgaggctgac accggaaagc gttttgatca
     7621 tggtgaccag gtcgctggcg ttaccataaa ggcgtttccc ggtacgccac attttctgca
     7681 cctgctcaac gaaatttttg cctgacgatg gtggcggcag aagtaccgag atatccccct
     7741 gcaacagcct ggcggcatcc gatacggcag aatccaccac tttcatcgca tcagaaacat
     7801 acccaagcat tgtgctggca ttaccgacga cgtcgttctg cacaaaatct gccacgccat
     7861 cgatactgaa accactgaag ctgtcactga tgcagtcatc cagtgcagaa caggatgaca
     7921 tcagcgtctg cgccgtcgcc gcacctgaag tggggtaaga gagttctccc gcttcgacaa
     7981 acttcaggtc aaagcggaca atacgccctt cactcttcga tgtgctgacc cgaacttccc
     8041 cgtcaacaca gactttcagc tcaccgtaag tcggatggac aagcgtgccg ggaccgggtt
     8101 tattcagcgc gtcaatcagg cgatcgcgct ggtcaaagca gtcatctccc accacataag
     8161 ctgtgatgga cgggcggaaa gtgattttcc ccaggtcttc ggtatagggt ttgtcgcggt
     8221 tcgggtattc gtgcgtttcc acacgacgac cggttcccgc actttcttct tcaaccttaa
     8281 acggcacacc gcgaaatgac gcgtcctgaa gtctgtcttt ccacgtcata taaactccgt
     8341 acataaaaaa tcccaccgga gtgggactca ttaacagatt aatttttcat tacctgccaa
     8401 agcgcgtata gccaacatca tggctgacat caaaaccgct ggatcgcgtt tccataaccc
     8461 gcatacccgg aggcgaattc acaaaagaga ccttgatctc accatcaact tttggcgcag
     8521 aagctttgtt aatcatgaag ggattcgggc ctgtggcacc ggaggcgttg tttgactgag
     8581 ccggatctac cgcctgataa ggtgtgtatc cccgtgccgg tattcccgtc ccataagcat
     8641 cataagcacc cgcgccccac tgcgcagagt taatggcatc gaccgtgtca ccggaactgt
     8701 cggtaaacca ctcaataatt ggcttcagtt tgtcccacat atcctgaaac cacttaacaa
     8761 ccggtcccca gttattgatt accatcccca gcggcgacca ggcaaaaacc ttcttcagaa
     8821 gttcccagcc agcctcaaaa taaggaccaa tggtttccca gagcttcttg aaataaggtc
     8881 cgacaacatc ccagttagtg ataattaatc ccgcagccag ggctatcgcc gtcgcaatca
     8941 ttccaatcgg cgtcatcgac atgatcctgc tgacaatact gatggcaccg ccaacgccca
     9001 tcaatcccag tttcagaatc gcaagaccgg cagcaagccc gacgacgccg cgaataaccc
     9061 ggggattttc atccgcaaac ttcgtgaatt tttcccctaa ctcccccagc cattgcgtga
     9121 tatttttggc gtcaccagaa aatgcgccgc caatagccgc aaggccgtta gttgcggtcc
     9181 ccgtcattgc ctcccacagg ttggacagcg taccaagctg ggcctgaaca cgtttattca
     9241 ggctggcctg tttattcatc ttctgctgga tctgatcgta gccatccttt cctttatcga
     9301 ttagtgcatt gaccacctga agggtttcgg catcatcacc aaatattgcc ttaagtacac
     9361 ctgttcgctt aacgtcggtc agttttcgca gctttgccag ttgcctgaac atgttatcaa
     9421 gaccgccaaa acttcctttg ccgtcagtaa aatcgagctg taccccgagt ttctggcggg
     9481 ccatgatttt attgacgtcc ctgattttct taacgcttaa tccggactgg ataacttttc
     9541 gcagggcgtt acctgccgac tccccgttca tccccatctg atccatcatg acgctgatgg
     9601 gggcaaggct ctgtgcagcc tgaagaccgt ccttgttcac catcttcaga acagagctgg
     9661 ttttagtgaa gaaggacaac atgttggtat cgtcaacacc cagataaaac gccttctgga
     9721 tagtgtcgaa cagccccatc atgtcttctg acgccgttcc ggtagcatcc tgcatctttg
     9781 cagcaaactc agcagccgct tccggtgttt ttttcagttg taccgcaaga taagctgtcg
     9841 ctttacccac accgccaaga atgttttctg ccgggatccc ctgacgcacc agcatctgca
     9901 tcatgttctg gaaatcagcc gttgtaccgg gtagctggtt acccaggcca atagccagtt
     9961 tattgatgtc ctgaaagctc tttccgacct cgccgttcgc atccatcatg gcgactttca
    10021 gcccggtggc ggcgttttcc tgatcggcat aagatttcag ggaaagcgtc agacccgctg
    10081 ccagtccgcc accaagcgcc agcccaccct gtgacgcttc ttccgcctgg cgtttaaatc
    10141 cccggatttt cttttgcatt ttcgacagcg cgggagaaag cctgtcgaca ccggtgatca
    10201 acgccttaag ctcaaattcc gccatgtgtg cgtttctcct gctctatcct gtttgcctga
    10261 ctgaccagca aggtaatttc actgatcggc atattcagca attcgaaagg attaatgcgc
    10321 cagtaactgg cgcagtcaaa gaagcgatca gtgaggtatt cagccgtcag gcctggagga
    10381 aaaaaccagc cacaagccac gccgctgcat tcaggtctgc cggagacatc tggtcgacag
    10441 agctttgcgg cactttcgcc agccgcacaa tgtatttcga caccacatgc gccagaagtc
    10501 tgactgactc atcctgattc atctggtagg gataccccag ctcgcggaca tccttcccgg
    10561 tgggttcatc aaactccagt acggagagtg tctcgccatg agcagtaatc ggtttcttta
    10621 actcaagctc tttcattact ggtaatcccc ttcttcaccg tggaactcaa gatcaaccgt
    10681 gccttcttcg gcattatggt tcgcttcgcc gtgcagccag gcagacgaca gtacatagac
    10741 ctgaccgttc gccagctcgg cagtgatggt catctcatca gacgaggtga ttttgcttac
    10801 cggaaaattc ttcggcacct tgaaggtccc tttgacataa ggcgcacggt gagtttcctt
    10861 gcggtccact gaaccgtcca ggccgatgat gtcatcgtta accgttttgt tcatgggcac
    10921 ctcaatgccg ccggtcagcg atagctgctg accgtcaatt ttgaaataac aggttccccc
    10981 gatacgggcc attatgcaga ctcctctgaa tactgaagac ggaactgatt aaccacggca
    11041 aagacacgca gctggttaac atagtcaggc gggaacagcg tgttcaggcg gttcggatcg
    11101 ctggcatcac gctccacaac caggtactgc ttaaacagtt cgtagttttc cacgatcccc
    11161 gcacgctcaa gctgacggta ggttgccagc agttcccctt tgatcaccgc cggggtgaca
    11221 atcgcctgac cgggaccaaa gcgggtaccg tcactggcaa gcttgtgacg cccgtactta
    11281 ctggtaatga cggatttcag tttgcgcagt acatacgcgc tggtatgcag cgtctcactg
    11341 tcgaggtaac tgttatccgc aaccccgtaa gcgtttttcc tgtacgtggt gacatcacgc
    11401 tgaatgcgta gtaccccgct ttcgacatac gccgttgcca cgccatgaga cagcagggtc
    11461 tgttgttcgg tcatcgtgaa ccgtttcccc ttcggcgcag gcagcatacc caccagctca
    11521 ccggtctgcg tgggacgtgc cggatcgttg cggataaaca ccgctgcgcg ggcggtacgg
    11581 cttgccgcca gctcgtcggc aggcgtctgg gtctcttttt cgtaccccgc cagggtaatg
    11641 tgctgctggt taaactggtc acctgcggtc accagttctg acagcgtgcc gatctttgcc
    11701 gtatacacat gaccatacag ctgacgcgca tagctccagc gaccgctggt atcgttcatc
    11761 tcggtcacca gcgtgttaac ggaggccgtg tcgttgaacg gcaggccgat ataatcaaac
    11821 ggctcatccg ccattgcagc caccgcgccg gtgagaaccg gagcacccgt tccggcggta
    11881 cccgtcgcca cggcaatctg tacgcccgct ggcagcactt cgcccccacc aaagccgtag
    11941 taattgaggc tgacaggaat ttcattccca caaagcccct tatgacgcgc ggtcagtgtg
    12001 accacgcctg ccgaagatga agccgtaaac ggcagggtcg gaacggcatt gatggcatcc
    12061 tagatactgc tggcaatcat cgtgacgtta tcgccgttag tcaccggtgc ctgcacgcgg
    12121 gtacgtccta catacacatt caccgtgccg gtttcggttg ccgccccggt caccgtcagc
    12181 gtaactgttg ccgccgcacc tgtggattca ggaacggcaa tcacatacag ctcgccaaac
    12241 gggtcagtct ggcgataagc ctcgaccata cgcgccagct gacttcccgc accacaaatc
    12301 tggcgtgcat agtctgccga cgacatcagt accagactgt tggcaacaat ctctgcaccg
    12361 ttattggcat gaccaatcag cagcgatgct ccgctgtcct gtgcagtatt cgccgcctgg
    12421 ttatccattt ccgcataaaa caacggaacc agcgtattcg acggaatggt gttaaagctt
    12481 atcgtcatcg gtgttcacct ttttattcac gcgccggata tcacccgctg cttcacggcg
    12541 cagccagtag ttgttctcgt caacatttcg cccttcggcg ggcaaaaggt cgccgcgggc
    12601 agggtcaggc actgaccgcc ctttaacagg tttgacaaac atgaggatcc tcaggaagga
    12661 agggttattt aggtgtgatg ttcgatatcg ccgtcaggcc cgttaccggg ctcgagataa
    12721 tcaacatcaa tcgccagcgt ttgcagttca tccagactgt tcagatcatc ctgctggcgg
    12781 gtatcgtctt cagtcagctc gctgatgacc gaaaaatcga actgataaat cagctcatga
    12841 cgattcagat ccagcagcgt gccgccgtca taggtaatcg ggttaccgca cgcctccggg
    12901 ttccagccca gcagagcctt aaagagcatc tgccggacat cgtccaccac atcatacgag
    12961 gcaaactgac cacgctcatc acgcccatta ctcagtatga caaccacgga gaaaccctct
    13021 ttcagctcct gccagtagtc ggtctggctt ttgttttctc ccggagaatc atcacccggt
    13081 acaacatatg ccgccgggag tttcagcttt ccgacctccg gcagattttt gaactgggcc
    13141 gcgcctgcaa cccggttttc aaaatacgga caacgggcac gcagtgcagc aataacagga
    13201 gtcagtttca tctgtgtcgt cgctccggct tcagtgattt acgcaattcc cgcgccagaa
    13261 aatagcgtgt ccagctgcgg ttcttttcaa gcgtttccac catgaagtta ttacgtggag
    13321 ccagtcgcca gccgctgcca ccggatgcac cacgatgatg actacgacga cgttttgctc
    13381 ctccccggac accaaaaaac agaaacgccg gatagaagtc accagagatc atccggttcc
    13441 ccttcccgtt gcgctggtta ggggcaatgc gtgtcataaa accggctcgc tttttactgg
    13501 ctctcggcac catataacca atcgaacgag ccaggcgtcc ggtctgataa ccggggtttt
    13561 cacccggtgc cgaccgcgca cggcgcatca ccagccgacg ggcatcacgc atatgacgct
    13621 gcccaatcgt gacaaacgcc cgccggacac gagcgcggtt aaagcgcatc tcggcgggct
    13681 gctgaacatc aacgtgaaaa aagggagtcg ccattgctgc ctccgtgact ctgcctacat
    13741 tcgcccagct ccgtacactc cagcagcaga aagcgccgcg ccccgttcag atcacgctga
    13801 cgtttcaccc ggtacacact gtcatcacag accacctcat aatcagcagt gatcccccgg
    13861 cggtaacgaa tggtgatgta atgggtgatg gcgtccccgg tctgcgcggt ttcctgccag
    13921 gtggtggcac tggtctggat aaccttcgcc catgtccgga acgtaaccgg gtattgaggc
    13981 tccacgccaa agttatccgc gggcatatcc acccgctggc ggatcaggac gcgtttattc
    14041 agttcaccgg ggtccggcag aatgtaggtt gcgctggtct gcgcctgacg aattttcatt
    14101 gcggaaagta cctgtacggg ccgacaagcc agccaaaact ctgcggcatg tcgagtttct
    14161 ccacttccgt aaccgacgag cggttttcgt aaaaatggct gataagcatc agcatcccca
    14221 gacgaatatc atccggcagg tgcagcccgt ccggatcgct gtccggaatg gtttcatccg
    14281 gtgcatagag cttccggttc agatacgttt ccgtccgctt ttgtgccgca caggccagca
    14341 gttgcagatg gcggtcatca gcatcgaaat cctcatccag ccggagttgg gctttaatct
    14401 cttccattgt cagaagcata ctcagccctc tttactggtc gtggcttttt tctcttttgt
    14461 cgctttactg ctttttgcac tggttccgcg ctctgctaac ccggcctgaa gtgcaatctc
    14521 ctgcacccgg gcaggaagcg ccccgtcgtc atactcaccg gcccgaatga cctcaacacg
    14581 cataccgtcc ggtgaccatt tcagatcttg tttcaggatc atgattcttc acccgtcaga
    14641 acaggggcgc ggttccgcgc ccctgagtga ttacgccact gcaatcttca gcagtttgat
    14701 ggcctgcgaa tcgaccagca tgccgccggt gcgtttggtg gtataaaaac cgacaaacgg
    14761 tttattggtg tacgggtcac gcagaatgcg ggtgccgata cggtcaacga tggtgtaacc
    14821 ccgtttgaag ttaccaaatg caatggcttt cgcatcagcg gcgatatccg gcatctgttc
    14881 gttttcagcg ataccgtaac ccgccagaga ggacggctgc cccagctcca gccccggacg
    14941 ccacagatag ttaccctcgc tgtctttcag cagacggatg gcaaacaggc tattgttgtt
    15001 catcatgaac ttcgcgccgg tgcggtgtgc cttacgcagc gtgtaaatca gtttgataat
    15061 ggcgtctgcg gtcaccgccg tcgcgtcgcc ggatacaata tgctgaagtt tgccgaacgc
    15121 ccggacctta tcggtttcat ccgtggattc attcgccagg aaccctttcg gcttcttggt
    15181 accatcgccg gtggtaaagg caatttcttc ctgttcggca aattcggttg ccagctcgct
    15241 gttgatccat gcttccacgt tgaaaaaggc atcatccagc attttctggg tggcctgcgg
    15301 g
//
LOCUS       DAJTNN010000094        15047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTNN010000094 DAJTNN010000000
VERSION     DAJTNN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.094"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..261)
                     /locus_tag="OO150_002552"
     CDS             complement(<1..261)
                     /locus_tag="OO150_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR5516650.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGCIKRQMFTKLRTTKYLKTAASA"
     gene            complement(254..685)
                     /locus_tag="OO150_002553"
     CDS             complement(254..685)
                     /locus_tag="OO150_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5516651.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVASGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSNVEAVAEPDEPEEMWTVKRG"
     gene            complement(781..1209)
                     /locus_tag="OO150_002554"
     CDS             complement(781..1209)
                     /locus_tag="OO150_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HCR5516652.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSVLLRQRQRRIEELKRENEDLRRWADT
                     PLPADIIRLRERPALTGGAAYRQWLSASDAVSAGAGSAAH"
     gene            complement(1206..1583)
                     /locus_tag="OO150_002555"
     CDS             complement(1206..1583)
                     /locus_tag="OO150_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516653.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALASLIKNGPLWQELRASLLKTITRKENVFISWVIRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITHNQMAQQVAA"
     gene            complement(1585..2097)
                     /locus_tag="OO150_002556"
     CDS             complement(1585..2097)
                     /locus_tag="OO150_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5516654.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALEKCVKQQPPQK
                     VYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(2078..2293)
                     /locus_tag="OO150_002557"
     CDS             complement(2078..2293)
                     /locus_tag="OO150_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516655.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(2297..2500)
                     /locus_tag="OO150_002558"
     CDS             complement(2297..2500)
                     /locus_tag="OO150_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCR5516656.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(2500..2964)
                     /locus_tag="OO150_002559"
     CDS             complement(2500..2964)
                     /locus_tag="OO150_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCR5516657.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERFQDYDATASGAKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(3060..3710)
                     /locus_tag="OO150_002560"
     CDS             complement(3060..3710)
                     /locus_tag="OO150_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HCR5516658.1"
                     /translation="MSLSPARQHRLRVQAEQAVREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLTEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(3714..4772)
                     /locus_tag="OO150_002561"
     CDS             complement(3714..4772)
                     /locus_tag="OO150_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HCR5516659.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQISDFSAAAAETGA"
     gene            complement(4789..5622)
                     /locus_tag="OO150_002562"
     CDS             complement(4789..5622)
                     /locus_tag="OO150_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCR5516660.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTFMEVSLKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            5765..7531
                     /locus_tag="OO150_002563"
     CDS             5765..7531
                     /locus_tag="OO150_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HCR5516661.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDDQIEKLEKIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGRLCADGQYRQIVT
                     VEDAVRGACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            7531..8127
                     /locus_tag="OO150_002564"
     CDS             7531..8127
                     /locus_tag="OO150_002564"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516662.1"
                     /translation="MGKSKKNRAAATKQTQHKNQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILISTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRR"
     gene            8161..8562
                     /locus_tag="OO150_002565"
     CDS             8161..8562
                     /locus_tag="OO150_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5516663.1"
                     /translation="MYMTDAAQNQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGL
                     QIIPLSEVAAKDEFLNIKNVSRDDMMAAHRVPPQMMGIMPNNVGGFGDVEKASLEFVR
                     NELIPLQKRMMEINKLVSENIVKFDSYKLAT"
     gene            complement(8669..9550)
                     /locus_tag="OO150_002566"
     CDS             complement(8669..9550)
                     /locus_tag="OO150_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516664.1"
                     /translation="MKWAIEMNKLKRPVFKDYSAFKKLSANKRLKHHSVLNGQDTFIL
                     NAYRSYIKNKGYLNVNSCGVLSNNIAEALRYYYNNPAKCLSIIDDIRAANSNSLCPMC
                     GSMHSGTLDHVLPKENYPEFSLFTKNLVPACKCNTFKSTTIANSAGHRMLHPYFDNIL
                     KQRLICANFSKLGRTPTIDVKIIIDPMSPEYNNVKYHLDNVVIKNNIKGYLSEQWESF
                     YLYPELVIRGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIRPDVIRW
                     ILCELHLGNRSPDGRLI"
     gene            complement(9534..11183)
                     /locus_tag="OO150_002567"
     CDS             complement(9534..11183)
                     /locus_tag="OO150_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5516665.1"
                     /translation="MLVKKNKNYSLYFLITNNQQISDSGNYIKIVPSRSDWNDFGYLS
                     RVEISIFNKKECKDIISLNGYIGFLNSEEERNGKAELIRRVKKKEESNLKDEGFNDYF
                     VMLQDMNNYRRLVRFFGVNESKYILNFINDVVSKKSDASAENLRKKAISSDIFNKSFI
                     RESESYFTFKNAGTVLSGIQYEELGNLSQHIKISYSNKVNEEDVSYVFNFDHEHDLPK
                     RISIIIGENGVGKSQTLREIALAAIKGKDNLVAVVDSNGVSIEERIQISRLIAFSPTN
                     ESKCSFPTDKRIKSLIWYKRLTLNRESKSKLNLNDLIVQLARSNDAIGYNQRWNIFVD
                     ALSVLNSNNDLVIKCGGENSPFVKLNELNSRGEESRLSLYSEIILSGEPLRLIENKCY
                     PLSSGEISFIKFCVQVCLYIENGTLLLIDEPETHLHPSFINKFMALLDNLLEKTGSSA
                     IIATHSVYLVREVFKEQVTILRRRDDGVIVSEKPRLSTFGANIGNISYFVFGESEPTQ
                     ILNKVKNNIIAKNMSWDDVESKYSEDLSLNVLTALRNEMGN"
     gene            complement(11476..11709)
                     /locus_tag="OO150_002568"
     CDS             complement(11476..11709)
                     /locus_tag="OO150_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCR5516666.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(11720..11908)
                     /locus_tag="OO150_002569"
     CDS             complement(11720..11908)
                     /locus_tag="OO150_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516667.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(12062..14476)
                     /locus_tag="OO150_002570"
     CDS             complement(12062..14476)
                     /locus_tag="OO150_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCR5516668.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFYESRDPVQHEMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCL
                     LAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     QWRNLY"
     gene            complement(14473..>15047)
                     /locus_tag="OO150_002571"
     CDS             complement(14473..>15047)
                     /locus_tag="OO150_002571"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HCR5516669.1"
                     /translation="YSTEITDFMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNP
                     YFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTED
                     DQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKVKRSIGVAAGEGKSAT
                     EIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT         3657 a         4079 c         3479 g         3832 t
ORIGIN      
        1 ggcgctggct gcagttttca ggtattttgt ggtgcgcagc tttgtaaaca tctgacgttt
       61 gatgcacccc tttttgctgc gtgctgttac cctgcgcggc tcataactgc tgccatcagg
      121 attgcgctgc atcctgatgt tctgctgctg tgtccggcgc agttcctgcg ccagctggcg
      181 catcatgcgg cttcttgcgg ctggctccag attcgccaat aatgcactca gccagtcgtc
      241 caccttctgc aattcagcca cgtttcaccg tccacatttc ttcaggttca tcaggttccg
      301 ctaccgcttc aacgttcgac acactgccgt cagtgctgac cagcacacgc tccgtcagtt
      361 gcaggttaag gctgatatca cagacatcgt tgcgcagaat atccacctca aaggtgaata
      421 gcttttcccg taacgccggg ttattgatcg catcaggctg gttatcccgc agccacagca
      481 aaaccggggc catcagcaga ttctggtcgc cgctgaaatc ttcaatcacc acgttcaggg
      541 tgtagcggta ctcccatgac atggagctag ccccggaggc aaccagcgaa ccgttatcca
      601 caaacagatg cagtttgtcc gggttattac ggacataagg cactgcttta ttgagggcgt
      661 ggcgcaggga ttgtggtttg ttcactgttt cgctcctgac acgcaataat catgtccact
      721 ttgtctgcac agaccgccca ggcggcctcc gtttcatcca gcaacgcatt caaatcaccg
      781 ttagtgcgcg gcgctgcctg ctccagccga cacggcgtca ctcgcggaca accactgacg
      841 gtaagctgca cctccggtga gtgccggacg ttcccgcagc cggataatgt cagcaggcaa
      901 aggagtatca gcccagcgac gtaaatcctc gttctcacgt ttcagttcct caatccggcg
      961 ttgtcgttgt ctcagcagta cactggtctg ttctgcttcg gcatagagcc gcgcctgctc
     1021 ccggttattg gtttcagtca gaatggacag gctgataagc tggctgttgc tctttgccag
     1081 tgcctggctt ttgctctgca gctcgtctgc ctgcgtgctg atggtctggc tggcatcagc
     1141 cagccgccac gtctgccagc ccagcgccgc caataataac gccagcacaa ccagcagcaa
     1201 ccggttcatg cggctacctg ttgcgccatc tgattatggg tgatccagaa ggcaataacg
     1261 gtcagcagat aaaagaccag ggtaatggcc caccccgtcc aggcgagact gacgacaatc
     1321 agcaatcgta tcacccagct gataaatacg ttttcttttc gggtaattgt cttcagtaaa
     1381 gatgccctca actcctgcca gagcgggcca ttcttaatta acgaagccag tgctaccgga
     1441 attaccgccc atgtcagtaa acaggctacc caaacgccgg acgctgccag taccggaaaa
     1501 atcccctgcg gatacaccat tgctgcgatt aacagcgcca tccataacat cagaaacagc
     1561 ccgctgatta atttcttttt catttcagtt tgctccctgt aaacaccagg ccatctcccg
     1621 cgcacggcgg ttatccagcc cctgattaaa aacacctttc acataaaccc agcgcggcaa
     1681 ctgtcggcac gcatccgccc agcgccgctg attgagcaat ttcaccagcg tggaactgca
     1741 ggcattgccc gtacccacgt tgaaggcaaa cgacaccgca gcgtcataca ccttctgcgg
     1801 cggctgttgc ttcacacact tttccagcgc ccgctccaca cgcagcacgt tggagatcag
     1861 cccttctgct gcctgtcgct ccgtaatggt tttgccggga atgacgcccg acgtattacc
     1921 aataccgtcg gtccagacac ccgcgctgca ctgatacggc tgcagacgac aaccttcgta
     1981 atcggcaatc agtttcagtc cctccacgga ggtgtgaagc tgctgaaaac ccggcagcgt
     2041 ggcagcaata gccagcacgg ccccgacaag gcagcgttta acgattgatg gattcatagt
     2101 cctcccgcga gatctgcccg tcgcgcagaa gctggtaggc tttgtgtttg tagtaccagt
     2161 tgatagccag catcagcaca ccaatcatca ggccgcccag cgttgaggca tccttgatgg
     2221 acaaatcgcc cagccaggcc agcacgacgg cgatgcaata cgtgataaag gcgctgattc
     2281 gctcaagcgt cataattcag tcccatagct ggacggtctg cacggtggtg gtggtcggaa
     2341 tgtccggcag ctccacctgc agcccgtgag gtaaaaaggg gccgtattcg gcaagccccg
     2401 gatttgcctt cagtacctgc tccgtgacac cctgcgtgcg cccgtaatga cgccagcaaa
     2461 gcgcgtccac cgtgtcatac tgatgcgcac gcactttcat cagataagct ccactgtgca
     2521 gtgcggcgcg tcctgcaccc ggctgatggc ccagcgggcg tcacgccaca aatcaccgct
     2581 tgcttccgcc agttcttcgc cccgcttcgc accggatgcc gtggcgtcat agtcctggaa
     2641 acgttcgttg agcatggcgc gagcccagca gtaaaccgcg ttgaaatagt gctgaatgcg
     2701 ctcacttttc ccgtccagct gttccgccgg aacctcagcc agcgacgcat accccagcat
     2761 ctgctggcgt ctgcgaaact catacagctc tgcgttaacc tccgaaattg ccgacagtgc
     2821 aacctgcttt aaacgcggct gcgtcaccgt gccgtcagtg cgcatgacac tgcgaaactc
     2881 cgacaggtcc acatcaggcc agaacggcgt atttctaatg atttccgcct gttccggtgc
     2941 ctgttctggc gcaacaaact tcatgctgct ttctcctgaa ataaagggcg gtggacgggg
     3001 ttttgatgtg gctgtgcctt tcgccacccc gtgccgcccg tgcgcggggg cacgttctgt
     3061 cagcggctgt cattgcgcag tctgcgctcc agctgctgtt tgtctttttt cacgccacag
     3121 cggggatcga gctgtaacgc atggttgaga tgattaaggg cggaagccgg attgctttca
     3181 ctcaggaccg cgccaatcgc tttatgcaga cgtgcccgtg actggtccgg catatccaga
     3241 ccgtctgtca gctccagcgt ctgcagcaac agatcggcat caaagccggt ggcggcaagc
     3301 attgcgctct gcgctgcatc tgccatttcc tctgtcagca cggtctgcac gttgcggttg
     3361 cccagcggca tcacccatcc atgacgcagg gcatgacgcc cgatctccag cgccccggca
     3421 taatctccgg catcaatgcg ccacaacatc acgtacatca gcacgtcatc ctgttgagcg
     3481 cctccggcag ccaggacacc ctccgcccag gcggcgtact tcggcagcag ctccactttg
     3541 atttccgctt ttttgaccgt ggactgaacg cccttgagac ggcggcggtc ttccgccagt
     3601 tgcagcagca tcaggtcata gcccgatgcg tggcgaacac tgccaccctc acggacggcc
     3661 tgttcagcct gaacgcgcag gcgatgctgc cgtgcgggac tcaggctcat ggattacgct
     3721 ccggtttcgg ctgcggcggc gctgaaatca ctaatctgga tgttttccac cagtgcggcg
     3781 cagcggtagt cctcaaccac ataggcttcg ttaacggatt caaagttttc aatccggtca
     3841 cgtttcgggt tgtcgataac tgaacggcgg cgggtgtctt cctgccagta gatggacagg
     3901 ttatccagac gggtgatcag cagcgcattc ggcgggaaga acggcgcacg cacggcctgc
     3961 aggccaccca tgcgtttctg actgatgatc atatcggcag ccagtttttc actgttttcc
     4021 tgctctttgt tgaccagcgg gaaatacttg tcagacagca gttcacgacc gcaaatcacc
     4081 accagatcgt catcgtcctg gtagaccacg tcgataagct cattgacggc atccatcacc
     4141 acggcgtcca ggttggcata ttcgccacct ttcccgactt tcactgcgcc cggtgtggtt
     4201 tcaccgcccg tggtggtgct gcccatgacg tgatccggtg catcctcacg gattttctgc
     4261 agccagcctt tattcacatc ctgcagcagc gggttttcgc tacggttgga ggttttcgca
     4321 cgcttcacgc cattaaagcc gatcatgatg cggtccagtg cctgacgttt cacgatggcg
     4381 tcacggatac gcacctgaaa atcctgaaac ttcgcccaca ggtccagctt cgcgtaggtc
     4441 agcaccgtgt caaagttggt ctgctcgcat ttatattcca catcgaccat cagcgtcgga
     4501 tcgacaggtt cacgctcttt cgcagtggta tcagtggttc cggcaatggt gctgccaact
     4561 cccaacccca gcagctgacc ggactgctca gtcactggcg tgacgttaat cagagtcagg
     4621 aaagcggcgg actgctggat ctggtcttcc agcgtctgct gcacggacgg ctctacggtg
     4681 aacttgctgg acagttcttc aactgccaca ccgttcagac gcgccagttg ctgcaggtaa
     4741 gcgttaaaag caaagcgggt attcttcttc atcaggtttt gtgctccatc agcaattggt
     4801 cagagtgtca gcgggggcgt taccgcctgt tgcacgctgg cggtagtcct ggcggctgtc
     4861 ttcatgactc agcttgtcca ccagttcgtt aaaggcggtt tgctgtgcct gcagggcagt
     4921 ctccagctca gacaggcgtt cttcctgctc agacagggat ttttcggtgc gtgcgctcag
     4981 gttctgctgc tcagtggcga ccagctccac ggccttatgc acatcagaga accgggcgtc
     5041 atcggactgc tcttttttgg taaacagcgc cgtgacgcgg gcaaacaggg acggtttgtc
     5101 gtcctggact tcttccagtt cgatcaccgt ttcctctgcg gcggtaaaga gattggcggg
     5161 attctgcttg cggtttgcca gcgggttatg ggctgcactg gcgctgaatg tcagcatttc
     5221 agtgcccaga ctggcagggt catcagtggc agccaggccg actaggtagg ctttgcccgt
     5281 atcagcgaac ttcaggctga cttccataaa ggtgaataat ttctggcctt ttttcaccag
     5341 ttccaccagg gactccgttg gctcaacgtc agcatacagc gccatcttgc ctgccagcgg
     5401 accttccgtg atttcttcag caaacagcgc cgtcaccttg ccgtagcggt taaaggtgct
     5461 gtccggcaga taagacttga tgtgctcaag gttaatcagc gcggtataca ccgccgggtt
     5521 atagctggct gccatctgtt ccagccattc acgctggatt tcgcgtccgt cggtggtggc
     5581 accttccacc ccgatgcgaa aacgctttgc tttcactgtc atgagccgtg ctccgttaga
     5641 aaaaacttac tggagcctta tggttgcggt gataggggca gtgaaacaat gcgcggtatt
     5701 tgtaccgaca accacacaaa ccgcaggcgg ggaaagcctt cattcaaggc tgtaggtttg
     5761 tgccatgaac accacactga cacccgcaga tctcgatccc cgtcggcagg ccatgctgct
     5821 gtactttcag ggataccgcg tagcccgcat tgctgaaatg ctgggcgaga aagttgcaac
     5881 cgttcacagc tggaaaaaac gcgacaagtg gggtgactat gggcctctgg atcagatgca
     5941 gctcaccacc gccgcacgct actgccagct catcatgaag gagcacaaag aagggaaaga
     6001 tttcaaagag attgacctgc tggcgcgcca gtctgagcgc cacgcgcgga tcggcaagtt
     6061 taacaatggc ggcaacgaag ccgacttaaa ccctaacgtc gccaaccgca acaaaggccc
     6121 acgccgtcag cctgaaaaga atgttttcac cgatgaccag attgagaagc tggaaaaaat
     6181 tttccattcc tccatgttca actaccagcg ccactggtgg gaagccggaa aaaccaaccg
     6241 catccgcaac ctgctgaagt cacgccagat cggcgcgacc ttctattttg cccgtgaagc
     6301 cctgattgac gccctgctta ccggacgtaa ccagattttc ctttctgcca gtaaggcaca
     6361 ggcccacgtc ttcaaacagt acatcatcga ctttgccaaa gaagtggagg tggagctgaa
     6421 aggcgatccg atggtgcttc ccaacggggc cacactgtat ttcctcggca ccaatgcccg
     6481 cactgcccag agttatcacg gtaacctgta tctggatgaa tatttctgga taccgaaatt
     6541 tcaggagcta cgcaaagtgg cttccggtat ggctattcac aagaaatggc ggcagaccta
     6601 tttttccaca ccatccagtc tgacccacag tgcttatccg ttctggtccg gtgcgctgtt
     6661 caaccgtggg cgcaacaaag ctgacaaggt ggacatcgac ctgtcccaca gcaatctggc
     6721 ccccggccgg ctgtgcgcag acgggcaata ccgccagata gtcaccgtgg aagatgcggt
     6781 gcgcggcgcc tgtaacctgt tcgaccttga ccagttgcgc atggagtaca gccctgacga
     6841 ataccagaac ctgctgatgt gcgagttcgt ggacgatctc gcgtccgtgt tcccgctcag
     6901 cgaattgcag gcgtgcatgg tggacagctg ggaagtctgg accgactttc atgcactggc
     6961 cctgcgcccg tttggctggc gcgaagtgtg gatcggttat gacccggcaa aaggtacgca
     7021 gaacggcgac agcgccggat gcgtggtggt ggcaccgcca gccgtgccag gcggtaagtt
     7081 ccgcattctt gagcgtcacc agtggcgcgg aatggacttt cgcgcccagg ctgacgccat
     7141 caaaaaactg actgaacagt acaacgtgac ctatatcggc atcgactcaa ccggcgttgg
     7201 tcacggggtt tacgagaacg tgaaagcgtt ttttcctgcc gtccgggagt ttgtctacaa
     7261 ccccaacgtt aaaaacgccc tggtactcaa ggcctacgac attatcagcc accgccgtct
     7321 tgagtttgac gccgggcaca ccgacattgc gcagtcattc atggcaatcc gtcgcgccac
     7381 caccgccagc ggcaaccgcc cgacctatga agccagccgc agcgaagaag ccagccatgc
     7441 cgatctggcc tgggcaacaa tgcacgcact gtttaacgaa ccgctgcagg gcgagtccgc
     7501 caataccagc aatattgtgg agattttttg atgggaaaga gtaagaagaa ccgcgctgcg
     7561 gcgacgaaac agacccagca taaaaaccag acttcagccg aagcattcag cttcggcgat
     7621 cccgttccgg ttctggaccg ccgtgaacta ctggactatg tggaatgcgt acagacagat
     7681 cgctggtatg agccgccagt gagttttgac ggactggcgc gcaccttccg cgccgccgtg
     7741 catcacagtt caccaattgc ggtgaaatgc aacattctga tcagtaccta catccctcac
     7801 ccgctgctca gccagcaggc tttttcacgt tttgtgcagg attatctggt atttggtaac
     7861 gcttacctgg agaaacgcac aaaccggttc ggagaggtca tcgctcttga gcctgctctg
     7921 gcaaaataca cccgacgcgg attagaccta gatacctact ggtttgtgca atacggcatg
     7981 accacgcagc cgtatcagtt cacgaaagga agcatctttc atctgatgga accggacatc
     8041 aaccaagaga tctacggcct gccaggttac ctttctgcca ttccgtcagc cctgctcaac
     8101 gagtccgcca cgctgttccg ccgatagtat tacattaacg gcagtcatgc aggcttcatc
     8161 atgtacatga ccgatgccgc gcaaaaccag gaggatgtga acaacctccg caatgcgatg
     8221 aaaagcgcca aaggaccagg taacttccgc aatctgttta tgtactcgcc taacggcaaa
     8281 aaggacgggc ttcagatcat cccgttgtca gaagtcgcgg cgaaggatga gtttctgaac
     8341 atcaagaacg tgagccggga tgacatgatg gcggcgcatc gtgtgccacc gcaaatgatg
     8401 gggattatgc ctaataatgt cggggggttt ggggatgtgg agaaggcgag cctggagttt
     8461 gtacgtaacg agttaatacc gttacaaaaa agaatgatgg aaataaataa attggtaagt
     8521 gagaacattg ttaaatttga tagttataaa ctagctactt aacgtaatca ctaaatgagg
     8581 ttctttaaca cagctaaaaa atttattaat agccagcagt ttttatgaat ttcttcagcg
     8641 tgcagataca cgctgaagat gcaaaaaatc atatgagacg accgtctgga cttctgttac
     8701 ctaaatgtaa ttcacagagt atccacctaa tcacatcagg tcgaattaac ccagctacaa
     8761 aaaccgaatt ccaattattt ttacctttat gtttttcatc caacagcaac aactcgcgag
     8821 tcaacaactc atatacatcg ctataagtag aaagactctt attcagtcct ctaataacaa
     8881 gctcaggata taagtaaaac gactcccatt gttcagacaa atacccctta atattattct
     8941 tgataacaac attatcaaga tgatacttaa cattgttata ttctggtgac attgggtcaa
     9001 ttatgatttt tacatcaatt gtaggtgttc gccctaattt agaaaaatta gcacatatta
     9061 atctttgttt taatatatta tcaaaatatg gatgcagcat ccgatggcct gcagaattag
     9121 ctatagtagt acttttgaaa gtattacatt tgcaagcagg aaccaaattc ttagtgaata
     9181 aagaaaattc tggatagttt tccttgggta aaacatgatc caaagtcccg ctatgcattg
     9241 aaccacacat tggacataga gagttagaat tagctgctct tatatcatca attatagata
     9301 aacactttgc gggattgtta taataatatc tcaaagcttc agcaatattg ttactcaata
     9361 cgccacaaga attgacatta agataccctt tattctttat gtacgatcta tatgcattaa
     9421 gtataaatgt atcctgtcca ttcagaacag aatgatgttt taatctctta tttgcagata
     9481 gttttttaaa tgcagaataa tctttaaaaa caggtctttt taatttattc atttcaattg
     9541 cccatttcat ttctcaacgc agttaataca ttaagtgata agtcctcgct atatttagat
     9601 tcaacatcat cccaagacat attctttgca attatattgt tttttacttt atttaaaatt
     9661 tgagttggtt cactctcacc gaaaacaaaa tatgagatat ttcctatatt cgccccaaaa
     9721 gtactcaatc tcggtttttc gctaacaatt accccatcat ctcgtcttct gagtattgtc
     9781 acttgctcct taaaaacctc ccgaacaaga taaaccgaat gcgttgctat gatagctgaa
     9841 gagcctgtct tttctaataa attatcaagc aatgccataa atttattaat aaatgaagga
     9901 tggagatgtg tttctggctc atctattaat agaagagtcc cattctcaat atacaaacag
     9961 acttgtacac aaaacttaat aaacgatatt tccccactac tcaaagggta acatttattt
    10021 tcaataagcc tcaaaggctc gccagagaga ataatttcag aatataatga tagtctagat
    10081 tcttcgcctc gagaattaag ctcatttaat ttaacaaatg gtgaattttc accgccacat
    10141 ttgattacta aatcgttgtt actatttaga acactcagtg catcaacaaa tatattccac
    10201 ctttgattat agccgatagc gtcgttactc ctcgctagtt gcactattaa atcattcaag
    10261 tttaatttgg attttgattc acgatttaaa gtcaatcttt tgtaccatat caatgatttt
    10321 attcttttat cggttggaaa tgaacactta ctttcatttg ttggagaaaa agcaatgagc
    10381 ctactaattt gaattcgttc ttcaatgctc acaccgttag aatcaactac agccaccaaa
    10441 ttatcctttc ctttaatcgc agccaatgcg atttctctaa gcgtttggct ttttcctaca
    10501 ccattctcgc ctataattat ggaaattctt tttggtaaat catgctcgtg atcaaaatta
    10561 aaaacataag atacatcttc ttcattaact ttattcgaat agctaatctt gatgtgctgt
    10621 gataaattac ccaactcctc atattgaata ccggaaagaa ctgttcctgc atttttgaaa
    10681 gtaaaatacg actcagactc tctaatgaaa gatttattaa agatatcaga agagattgct
    10741 tttttcctca aattctctgc actagcgtca cttttcttag aaacaacatc attaatgaaa
    10801 tttaatatat atttggattc attcacccca aaaaatctta ctagtcttcg ataattattc
    10861 atatcctgaa gcatgacaaa ataatcatta aacccctcat cctttaaatt agattcttcc
    10921 tttttcttca ctcgccttat cagttctgct tttccatttc gttcctcctc tgaattcaaa
    10981 aaaccaatat aaccattcag tgaaattata tccttacact cttttttatt aaaaatagat
    11041 atttcgactc tactcaaata tccaaaatca ttccaatcgc ttcttgatgg gactattttt
    11101 atataattac cagaatcaga tatctgttga ttatttgtaa ttaaaaaata taaactataa
    11161 tttttatttt tcttgactaa cattattacc ctctcacttc attagtgatg atttattttg
    11221 gaattatttt acacacttat acctagcgcg caatcgtatc cccgccacgc ctgcccgctt
    11281 tatgtagagg ttttcatgca cctgcatgat ctacgcaaaa gcccgccagt tctggcgggc
    11341 cttagcaaaa acgatcctca aacgatcatg cgatctcatg cggcatagac atgcactaca
    11401 gagctaacgc ctcgcaaggg ctcgttgttc aaccttgctg acgccagaag ctagttcaga
    11461 cgccagcaac gtttcttaat gcagccagct gtcgtcttcc cacaccttct gcataatttt
    11521 catcacttgt tttctttctt cgtccagttg caatccagta agttccacac cgttagagct
    11581 acctttgcgg atacgaatta ccgttttggg atacagaggg cgcagattgc ggtaaagctc
    11641 ggattcaaga gcgtccaggg tagactggct aatcttctgc tctttatcga tcattatttc
    11701 aatgcgcata aaagtcacct cagctgatga catccattga gcggttgtat tcgtgggttc
    11761 tgatttttgc catgagttca tcagtcaatt cagaaaccca ctgcagagcc agccccttct
    11821 cttcatcact acactcacta gccgctacaa gcttaagaaa aaaatcaatg cgctggagct
    11881 tcaaagactc caaaaaatag tcctgcatct ttcctcctat gacaccacac gcaatactgt
    11941 atgcataacc actgtttata tttacagcat ataataatct tactgatgta aaacgttttt
    12001 ttacgttcat cagcctgata tgcctggtat tattaagagc acgaattgtt aacccgcgta
    12061 attaatacag gttccgccac tgatcatctt cctgcaaacg ctggttccga tagaagatac
    12121 gcaggcctgc tcctgacgga atactgcctc cgcgaaggag taaatcgacc tctttctcgc
    12181 tgccatcaaa tcctctggac ttcagctcat acacgagctg cagtcgctga tggtctgtaa
    12241 ttcgctgttt gtagtcttta cgccgtttcg gtttcaccag gcgtaacctt gctgccagtt
    12301 cccggcgctc ttttttgctc atactgtgca ggtaatcgtg caactccttg tcatccatgc
    12361 tggtaatgtc cgttctgggg ttcccatcag ctgatttatc tttctcctgt tggttcaaat
    12421 tttcagcaag gagacagtta ttgccacgag tccaaggggc gcaagcgccc tggtcggctg
    12481 ccgcctcctg aacgtcaacg gctttacgaa ccattttcca cttcactgca tgagtgcaga
    12541 tcttgccttc tgcaatgggt gaccagatgc cataaatacg aataccgtga tcgccatagg
    12601 cggtcggctc ttcattgatt tcataagcgg ttctgatcaa gtgatatttg cgaggaacca
    12661 gcacgccgcc ctgcctcata atgtaggtgg caaaacagcc agcatcagca gcagccagga
    12721 tggcatcaag gcgcgggtta tccagtaccg gcgcacctgc ttttttgtca ccctgctgcc
    12781 ttgccgcctg accagccaac aaccgcagtt cacggtaagc ctgacgccct ggaataccaa
    12841 agaagcggaa ttgctgaaca cgatgcagag acgcccaggg attaacgtat tcagcgttat
    12901 cacgcaggga tttacccgtt tccttgctga tctcgccagc cagaccacgc ccgtcaatgt
    12961 tcttactgat gtatttcgcg atgtagcttg tcggcgttcc tttgcgcggg tttatcagct
    13021 cagatttaaa gcgtggacca gtgttattac ccagttcctc gcggtcttca cggatggcaa
    13081 acttacgcaa caatgcagta atggcgcggc ggtctttttt gcgcataaaa cacaacaggt
    13141 gccagtgaac tgtaccgtca tgatgcggct cagccacccg cacgccatac cagcgcaacc
    13201 cggctttgtg catcgcctta cgaaatgcag caaacatgcc gaccagataa tcgctgcttt
    13261 gtcttaccgt cgcatttgtc caggttgggt ttggtctgcc gttattgagc gtggaatgga
    13321 aacgtgacgg acaggtaatg gtgtagaaaa cggcgcagtc accgcgcatt tccgcgataa
    13381 gctccagacc tttaacacag gccatcatct cattgcggcg gtgcgccggg ttgctgctgc
    13441 tggcgtttac cacgtcttcc atatccagcg tgtcgccctc ttcgttcacc agttcatgag
    13501 aacggaaaaa ctccaatgac ttgcggcgct gctcacgttt atgcatcacg gcttcatagc
    13561 tgacataggg agatgctttt ttgctgacca ggcagacagc acgcaactgc tcttcccgcc
    13621 attcgcaacg cattttccat aatttccggt accaccagtc ggcgcacaac atacgcgcca
    13681 gcgaacccgg aatgagttca tagggcacgg gtttacggcg gtttcttttc cgacggagtt
    13741 gctcaaacgc aggcgggatg acatccagac gcagggtttc cgctgccacc ttttcccatg
    13801 tcttgcggat ttcttctggc ttaacgtcat cggtggcata caaatcacca caagctgcat
    13861 caaggcacat gctcatatgc gcagcgacaa gggtagacag gcgtttcacc tgatcctgac
    13921 tcatttcagg cagaatcagc aggccgtcca gccctacatg gcttgccata aaacgaaaag
    13981 atgcagatag ctggctgtcg cgtacatgct ccagtcgttc cagacatggc ttaatcgtct
    14041 cacgtaaata gcgggaataa gcctttggcc tgcccaggct gctgaagtat tcaatacgtt
    14101 gcatcagcgg cttgctgata tgggaaggct gggcgttgac gtccgccaga atgaccatat
    14161 ccggattaaa acgctgctgc tcatgcgcca gctttgcccg gctaatgagc ttatcctgct
    14221 ccatttcgtg ctggacagga tcacgtgatt cataaaaaaa ataacgctcc cagacctgat
    14281 cactcagtgc ctcgcggcgc aactgttcct gctcgttatc ggcagcgtac agagtgatca
    14341 ggtttgaaag cgcagaaacc ggcgcaactt ccgccgggtc cagataaggg ttaatggcct
    14401 ttttcgggct gttccatgag aacgctgcgg cagcctcgtt aaagccgcag cagttgttca
    14461 tatcggcatg gctcatgcac gtactccgta cacggcagaa ctatccacgc cacgcgaata
    14521 atcaaatccc acccagcagc gcggcccgga aacagcaatg atttctgttg ctgatttacc
    14581 ctcgccagct gccacaccga tgctgcgttt taccttgata tagtggtgag taaaattgcg
    14641 atacagcgaa cggatcaggg atgtgtcact gttagaaaca atgaccggat gtccttctga
    14701 tgaccgatgt tcaagaacgg atgctaggtg atactggtca tcttcagtga aaccatcagt
    14761 gtgatagccg gaaaacgtac cgtcatacgg tggatcgcaa tacaccacat cccccgcctt
    14821 caacatcgcc agcgtttcat caaagctggc gcagataaac gttgctcgct gggctttttc
    14881 tgcaaatgcg cgaatttctt tttcagggaa atacggattt ttataattgc cgtagggaat
    14941 gttgaaatgc ccgctcttgt tatagcgaca taaaccacgg taaccgtgac gattgagata
    15001 caggaaatat accgctttca tgaaatcagt aatttcagtt gagtaat
//
LOCUS       DAJTNN010000095        14940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTNN010000095 DAJTNN010000000
VERSION     DAJTNN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.095"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..454
                     /locus_tag="OO150_002572"
     CDS             <1..454
                     /locus_tag="OO150_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR5516670.1"
                     /translation="LQEVNAALFKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISI
                     ADPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMM
                     TLGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(495..1427)
                     /locus_tag="OO150_002573"
     CDS             complement(495..1427)
                     /locus_tag="OO150_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5516671.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            1791..4577
                     /gene="polA"
                     /locus_tag="OO150_002574"
     CDS             1791..4577
                     /gene="polA"
                     /locus_tag="OO150_002574"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5516672.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNPLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(4959..5591)
                     /locus_tag="OO150_002575"
     CDS             complement(4959..5591)
                     /locus_tag="OO150_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5516673.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRHSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6173..6682
                     /gene="yihI"
                     /locus_tag="OO150_002576"
     CDS             6173..6682
                     /gene="yihI"
                     /locus_tag="OO150_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5516674.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            6871..8244
                     /gene="hemN"
                     /locus_tag="OO150_002577"
     CDS             6871..8244
                     /gene="hemN"
                     /locus_tag="OO150_002577"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5516675.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8434..8544)
                     /locus_tag="OO150_002578"
     CDS             complement(8434..8544)
                     /locus_tag="OO150_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5516676.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8655..10064)
                     /gene="glnG"
                     /locus_tag="OO150_002579"
     CDS             complement(8655..10064)
                     /gene="glnG"
                     /locus_tag="OO150_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5516677.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKKIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10076..11125)
                     /gene="glnL"
                     /locus_tag="OO150_002580"
     CDS             complement(10076..11125)
                     /gene="glnL"
                     /locus_tag="OO150_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5516678.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11299..12708)
                     /gene="glnA"
                     /locus_tag="OO150_002581"
     CDS             complement(11299..12708)
                     /gene="glnA"
                     /locus_tag="OO150_002581"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5516679.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13081..14904
                     /gene="typA"
                     /locus_tag="OO150_002582"
     CDS             13081..14904
                     /gene="typA"
                     /locus_tag="OO150_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5516680.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
BASE COUNT         3864 a         3746 c         3851 g         3479 t
ORIGIN      
        1 tctgcaagaa gtgaatgctg cattgttcaa agagtattta ccgttattac aacaaagtga
       61 gccgaccatt aaacaaccgg taagatggaa gaacgcactc ggcgaactaa atgccaatct
      121 ggatatcagt attgccgacc cagccaaatc ttcatcatcc acaaacaaag atatcaaatc
      181 gctcaatttt gatgtgaagt taccgcttaa tgtcgtcaca gaaaccgcaa aacagcttaa
      241 tttatctgaa ggaatggatg cggaaaaagc gcaaaagcgg gctgataaac aaatcagcgg
      301 gatgatgacc ttaggtcaga tgtttcagtt aatcacgatt gacaacaata ccgcctcgct
      361 gcaactgcgt tatacaccgg gtaaagttgt ttttaacgga caggagatga gcgaagaaga
      421 atttatgtct cgtgccggac gttttgttca ttaaaatact cactcgcctc tgctcattca
      481 gaggcgagca aaatttactt gtcctgccgc tgtgatgaca tcaacgacgt cagaagtcgg
      541 tctttttcct gccacagtga gttcagccat tgctgaaaat ggcgcttaaa gcttttgtca
      601 ttgatgtagt cgccatgtaa ctcatcggca ataggctgta aatccacatg aacgacaatt
      661 cgcgtcagtt taccgcttaa catatcgaag aacggctgac ggttattgtc cggataacac
      721 agcgtaacat taagcagttt atcgaattgt ttacccagta catttagcgc catcgcaatg
      781 cctgccgcct ttggtggcaa caagttttga aaagtggagt gggtttgctg atgtttttct
      841 tgcgtgaaac gggagccttc aacgaaattg acaatggtgg tgggatgcag gcgaaacttt
      901 tcacaagaac gccgagtggt ttcaacatct ttaccacgtc gctccggatg acgtaataaa
      961 taagcgcggg aataacgctt cataaacggc atatccagcg cccagcacgc caggccaaga
     1021 aatggcaccc aggccagctg ttgcttgagg aaatatttat tcatcggaat gtgcttacga
     1081 aacagcacgc acagtacgac aatatctgcc caactacggt ggttacaaat aagcagatac
     1141 cagttcttct tacttaaccc ttccagcccg tgaacttccc attgcaggtg tgggttcaga
     1201 tgcagtaata ccgccagacc ttcacaccag caatacatca taaaatcaca aaaacgcgaa
     1261 acctttcgcc aaatgactgg cacaggtagc aatagtttta caatcccggc aatgatgatc
     1321 gggacggaac aaaaaatagt gaccaaaatg gtcaatacga tactcagcaa aagggttatc
     1381 gcagcgagta ttctcgtcat aatgaattta ttcaaaaggt tagccataga cagtgcgcta
     1441 aagaagcgca aggcgcagat tttagcagaa aacgacccaa ataacggatg atccttaagg
     1501 agaaaaataa ttcatatcta tccacattag aaaaaatccc attatctcaa ttattaggga
     1561 tggctttatt tttaactaca tgaaaaacaa agacaaacat catactgtaa aaagcatgat
     1621 aataaattaa aagcgatgta aataatttat gcacaaagtt atccacatga cgatttgcga
     1681 gcgatccaga agatctacaa aagattttca cgaaaagcgg tgaaaaactc atgtttttat
     1741 cctgtctgtg gcatccttta cccataatct gataaacagg cacggacatt atggttcaga
     1801 tcccccaaaa tccacttatc cttgtagatg gttcatctta tctttatcgc gcatatcacg
     1861 cgtttccccc gctgactaac agcgcaggcg agccgaccgg tgcgatgtat ggtgtcctca
     1921 acatgctgcg cagtctgatc atgcaatata aaccgacgca tgcagcggtg gtctttgacg
     1981 ccaagggaaa aacctttcgt gatgaactgt ttgaacatta caaatcacat cgcccgccaa
     2041 tgccggacga tctgcgagca caaatcgaac ctctgcacgc gatggttaaa gcgatgggac
     2101 tgccgctgct ggcggtttct ggcgtagaag cggacgacgt tatcggtact ctggcgcgcg
     2161 aagccgaaaa agccgggcgt ccggtgctga tcagcactgg cgataaagat atggcgcagc
     2221 tggtgactcc aaatattacg cttatcaata ccatgacgaa taccatcctc ggaccggaag
     2281 aggtggtgaa taagtacggc gtgccgccag aactgatcat cgatttcctg gcgctgatgg
     2341 gtgactcctc cgataacatc cctggcgtac cgggcgtcgg tgaaaaaacc gcgcaggcat
     2401 tgctgcaagg tctgggcggg ctggatacgc tgtatgccga gccagaaaaa attgctgggt
     2461 tgagcttccg tggcgcgaaa acaatggcag cgaagctcga gcaaaacaaa gaagttgctt
     2521 atctctcata ccagctggcg acgattaaaa ccgacgttga actggagctg acctgtgaac
     2581 aactggaagt gcagcaaccg gcagcggaag agttgttggg gctgttcaaa aagtatgagt
     2641 tcaaacgctg gactgctgat gtcgaagcgg gcaaatggtt acaggccaaa ggggcaaaac
     2701 cagccgcgaa gccgcaggaa accagtgttg cagacgaagc gccagaagtg acggcaacgg
     2761 tgatttctta tgacaactac gtcaccatcc ttgatgaaga aacactgaaa gcgtggattg
     2821 cgaagctgga aaaagcgccg gtatttgcat ttgataccga aaccgacagc cttgataaca
     2881 tctctgctaa cctggtcggg ctttcttttg ctatcgagcc aggcgtagcg gcatatattc
     2941 cggttgctca tgattatctt gatgcgcccg atcaaatctc tcgcgagcgt gcactcgagt
     3001 tgctaaaacc gctgctggaa gatgaaaagg cgctgaaggt cgggcaaaac ctgaaatacg
     3061 atcgcggtat tctggcgaat tacggcattg agctgcgtgg gattgcgttt gataccatgc
     3121 tggagtccta cattctcaat agcgttgccg ggcgtcacga tatggacagc ctcgcggaac
     3181 gttggttgaa gcacaaaacc atcacttttg aagagattgc gggtaaaggc aaaaatccac
     3241 tgacctttaa ccagattgcc ctcgaagagg ccggacgtta cgccgccgaa gatgctgatg
     3301 tcaccttgca gttgcatctg aaaatgtggc cggacctgca aaaacacaaa gggccgttga
     3361 acgtcttcga gaatatcgaa atgccgctgg tgccggtgct ttcacgcatt gaacgtaacg
     3421 gtgtgaagat cgatccgaaa gtgctgcaca atcattctga agagctcacc ttgcgtctgg
     3481 ctgagctgga aaagaaagcg catgaaattg caggtgaaga atttaacctt tcttccacca
     3541 agcagttgca aaccattctg tttgaaaaac agggtattaa accgctgaag aaaacgccgg
     3601 gtggcgcgcc gtcaacgtcg gaagaggtac tggaagaact ggcgctggac tatccgttgc
     3661 caaaagtgat tctggagtat cgtggtctgg cgaagctgaa atcgacctac accgacaagc
     3721 tgccgctgat gatcaacccg aaaaccgggc gtgtgcatac ctcttatcac caggcagtaa
     3781 ctgcaacggg acgtttatcg tcaaccgatc ctaacctgca aaacattccg gtgcgtaacg
     3841 aagaaggtcg tcgtatccgc caggcgttta ttgcgccaga ggattatgtg attgtctcag
     3901 cggactactc gcagattgaa ctgcgcatta tggcgcatct ttcgcgtgac aaaggcttgc
     3961 tgaccgcatt cgcggaagga aaagatatcc accgggcaac ggcggcagaa gtgtttggtt
     4021 tgccactgga aacagtcacc agcgagcaac gccgtagcgc gaaagcgatc aactttggtc
     4081 tgatttatgg catgagtgct ttcggtctgg cgcggcaatt gaacattcca cgtaaagaag
     4141 cgcagaagta catggacctt tacttcgaac gctaccctgg cgtgctggag tatatggaac
     4201 gcacccgtgc tcaggcgaaa gagcagggct acgttgaaac gctggacgga cgccgtctgt
     4261 atctgccgga tatcaaatcc agcaatggtg ctcgtcgtgc agcggctgaa cgtgcagcca
     4321 ttaacgcgcc aatgcaggga accgccgccg acattatcaa acgggcgatg attgccgttg
     4381 atgcgtggtt acaggctgag caaccgcgtg tacgtatgat catgcaggta cacgatgaac
     4441 tggtatttga agttcataaa gatgatgttg atgccgtcgc gaagcagatt catcaactga
     4501 tggaaaactg tacccgtctg gatgtgccgt tgctggtgga agtggggagt ggcgaaaact
     4561 gggatcaggc gcactaagat tcgcctgaac atgccctttt ttcgtaagta agcaacataa
     4621 gctgtcacgt tttgtgatgg ctattagaaa ttcctatgca acaactgaaa aaaaattaca
     4681 aaaagtgctt tctgaactga acaaaaaaga gtaaagttag tcgcgtaggg tacagaggta
     4741 agatgttcta tctttcagac cttttacttc acgtaatcgg atttggctga atattttagc
     4801 cgccccagtc agtaatgact ggggcgtttt ttattgggcg aaagaaaaga tccgtaatgc
     4861 ctgatgcgct atgtttatca ggccaacggt agaattgtaa tctattgaat ttacgggccg
     4921 gatacgccac atccggcaca agcattaagg caagaaaatt attcgccgtc ctgcgtttct
     4981 tctacaggct gcatctcgct aaaccaggta tccagtttct gccgcagctt gtccacgcct
     5041 tgtttcttca acgaagaaaa cgtttcaacc tgcacatcac cgttaaacgc cagtacagct
     5101 tcacgcacca tattcaattg cgctttacgt gcgccgcttg ccagtttgtc cgctttggtc
     5161 agcagcacca gaacggcgat attgctgtct accgcccact caatcatctg ctgatccaaa
     5221 tctttcagcg gatggcgaat atccattagc accaccagac cttgcaggct atgacgtttt
     5281 tcgaggtatt cgccgagcgc acgctgccat ttgcgcttca tctcttccgg gacttccgca
     5341 taaccgtacc caggcaagtc aaccagacgc ttgccgtcag ccacttcaaa caggttgata
     5401 agctgggtgc gccctggggt ttttgaggta cgagccaggc ttttctggtt agtcagcgtg
     5461 ttcagcgcgc tggatttacc tgcgttggaa cggcctgcaa aagccacttc aattccggta
     5521 tcggaaggta ggtggcgaat atcaggcgca ctcatcacaa aatgcgtctg ttgataattc
     5581 aaattagtca aagcggtcgt ctccgtcagt caaagctttg tggcgattat acctgaacca
     5641 taataaaagg cggatttttc ggcgtgagcg ttgtaagtaa aaaccatacg ctttgtgaga
     5701 cattgccgat agtctttatg cgaaatagca gagaaaattc tgcgatgcat gtcaaataag
     5761 ctatataaat cagtgaattg cttttatata aaactctgaa aatgtgcaaa tactgatggc
     5821 ggttgattgt ttgtttaaag cgaaggcgta aagtagcacc catagagcga ggacgctaac
     5881 aggaacaatg actcaggatg agggtcagga gcgccaggag gcgaagacag aggattgtca
     5941 ggaagacaaa cgtccggaga cgtaattaaa cggaaatgga atcaacacgg attgttccgg
     6001 ctaaaggaaa aacagggtgt gttggcggcc tgcaaggatt gtaagacccg ttaagggtta
     6061 tgagtcagga aaaaaggcga cagagtaatc tgtcgccttt tttctttgct tgctttctgt
     6121 tagattccgc cgcaaatcga tactgaataa aacggctaaa agacgaacca ttatgaaacc
     6181 atcatcttca aactcacgca gcaaaggtca tgcaaaagcg cgtcgaaaaa cacgcgagga
     6241 gctggatcag gaagctcgtg atcgcaagcg tcagaaaaaa cgtcgtggtc atgcgccggg
     6301 cagccgcgca gcgggcggaa acaccacgtc aggcagcaaa ggccagaacg caccaaaaga
     6361 tccacgtatt ggcagtaaaa cccctattcc attgggcgtg actgaaaaag tcaccaaaca
     6421 gcacaaaccg aagagtgaga aacctatgct ttcaccgcag gcggagttgg aattactgga
     6481 aacggatgag cgtctggatg cgctgctgga acgtctggaa gcaggcgaaa ccctgagtgc
     6541 cgaagagcaa tcctgggtgg atgctaaact ggatcgtatt gatgagttga tgcagaaact
     6601 cggcctctct tatgacgatg acgaagaaga ggaagaagac gagaagcaag aagacatgat
     6661 gcgtctgctg cggggcaact aacggattgc cgccgtgggc cttcccgttc tgctgataac
     6721 ccttccggtt atatgttatc tggtgtggtt attcgttaaa ctacagcggt tgtcgcggcg
     6781 acaaaagtgg ctgcgcaacc ggctgatgac ccgaaacggg catccgccgg tacgccgtag
     6841 ccgccagaga cgccatcgga aggagtgagc atgtctgtac agcaaatcga ctgggatctg
     6901 gccctgatcc agaaatataa ctattccggg ccacgataca cctcgtaccc gaccgcgctg
     6961 gagttttcag aagacttcgg cgaacaggcg tttttacaag ccgtggcgcg ctatcctgag
     7021 cgtccattat ctctctacgt acatatcccg ttctgccata agctttgtta cttctgcggt
     7081 tgcaataaga ttgttactcg ccagcagcac aaggccgatc agtatctgga cgcgctggag
     7141 caagaaatcg tccatcgtgc accgctgttc gccgggcgtc acgtcagcca attgcactgg
     7201 ggcggcggaa cgccgacgta tctgaataaa gcgcaaatca gccgcctgat gaagctgctg
     7261 cgcgaaaact tccagttcaa tgccgatgcg gagatttcga tcgaagtcga tccgcgggaa
     7321 atcgaactgg atgtactcga tcatttacgc gccgaaggct ttaatcgcct gagcatgggc
     7381 gtgcaggact tcaacaaaga agtgcaacgt ctggttaacc gcgagcagga tgaagagttc
     7441 atctttgcac tgcttaacca tgcgcgtgag attggtttta cctccaccaa catcgacctg
     7501 atttacggcc tgccgaaaca gacgccggag agtttcgcct ttaccctgaa acgtgtggcg
     7561 gagctgaacc ccgatcgtct gagtgtcttt aactacgcgc atctgccgac catttttgct
     7621 gctcagcgca aaatcaaaga tgctgacctg ccgagtccgc agcaaaaact cgatatcctg
     7681 caggaaacca tcgccttcct gacgcaatcg ggctatcagt ttatcggtat ggatcacttt
     7741 gcccgtccgg atgacgagct ggcggtggcc cagcgtgaag gcgtgctgca tcgtaacttc
     7801 cagggctaca ccactcaggg cgataccgat ctgctgggga tgggcgtttc cgccatcagc
     7861 atgattggcg actgctacgc gcagaaccag aaagagttga agcagtacta tcagcaagtg
     7921 gatgaacaag gcaatgcgct gtggcgtggt attgcgctaa cgcgtgatga ctgtattcgc
     7981 cgcgatgtga ttaagtcgct catctgcaac ttccgtctgg attacgcccc tattgagaaa
     8041 cagtgggatt tgcacttcgc tgattacttt gcggaagatc tcaagctgct cgccccgtta
     8101 gcaaaagatg ggctggtgga tgtggatgag aagggaatac aggtgacggc gaaaggtcgc
     8161 ttgctgatcc gcaacatttg catgtgcttt gatacctatc tgcgccagaa agcgcggatg
     8221 cagcagttct cacgggtgat ttgaatagtg aatggcgctt cgtttacaaa gtagaaaacg
     8281 aaagcgccat caatgtgctg agaacaagat tgcctgatgc gctacgctta tcaggcctac
     8341 aaaacctgtt gaaaatattg cttttgtagg ccggataagg cgttcacgcc gcatccggca
     8401 tgaacaaaga gcacttcatc agcaatctga aactcaacta aacagcccaa tcattgcggc
     8461 acacagcacg gcagcaacgg cagtttgcgg tgtgtggctg tcaactgcgc cactcgatac
     8521 cagattaatt attgattcca gcataatgac tctccccgtt ttccgggcag atcatactga
     8581 acttatcgga acagtaaagc gtaaaatacc agcaatttgc gctcaataat caatctttac
     8641 acacaagctg tgaatcactc catccccagc tcttttaact tacgcgtcag ggtgttgcgg
     8701 ccccagccga gtagccgcgc cgcttcctgt ttatgcccct gcgtatgtcg caacgcggtc
     8761 gtcagtaacg tccgctccag ctctggctgc gcttcggaaa gcagattttg atgaccggaa
     8821 cgcagcgctc tgtctgccca ctgcgcaaga agcgtcgccc agctgtccgg ttgcatttgc
     8881 gaagtactct ccgcaaccgt tgattcaaac agttcgccgg gcaaatcctg aatcaacact
     8941 tcctgcccgg cggccatcac cgttagccag cggcaggtgt tttccagctg gcgcacgttg
     9001 cctggccacg ccagacgcgt cagagcagct tcggtttccg gatgcagtaa cttcgcttct
     9061 acgcccagtt cgcgcgcggc aacctgtaaa aaatggcgcg ccagacgggg aatatcttcc
     9121 cgacgttcgc gcagcggcgg cagatgaacg cggataacgt ttaggcggtg gaacagatcc
     9181 tcacggaact taccttcctg cactcgctgt tcgagattct ggtgagtggc agcgataatc
     9241 cgcacatcca ctttcaccgg cgcatagccg ccaacgcggt aaaactgacc gtctgccagc
     9301 acgcgcagca aacgcgtctg cacatccagc ggcatatcac caatttcgtc gaggaataat
     9361 gtaccgccat cggcctgttc aaaacgcccc tgacgaatgg tattcgcgcc agtaaacgcg
     9421 cctttctcgt ggccaaacag ttctgattcg atcaaatctt ttgggatagc tgccatattc
     9481 agcgcgataa acggtgcttt ggcgcgcgga ctgtggcgat gcagggcatg agcgaccagt
     9541 tctttaccgg tgccggattc gccgttaatc agcacgctaa tagaagaacg cgaaagccga
     9601 ccgataatac ggaacacgtc ctgcatggct ggcgcttcgc cgatgatatc ggtcgttggg
     9661 ccgttaagct gaatattacg cggctgctgc tgttcctggt aatgactgat agcgcgctca
     9721 accagcgcca cggcttcgtc gatatcaaac ggtttgggca gataatcaaa cgccccttgt
     9781 tgataggcgc tgacggcagc atccagatcg gaatgtgcgg tcataatgat gaccggaagc
     9841 attggatggc gctgtttaat cttcttgagc agcgccagcc cgtccattcc cggcatacgg
     9901 atatctgaaa gcagcacatc cggcgttttg ctcgccagcg cctccagtac ttccgcgccg
     9961 ttctcaaatg tcgtacaggt taaacccgct ccagcgagcg cacgttcaag cacccaacgg
    10021 atggaactat cgtcatcgac tacccagact atccctcgtt gcataaacgt cacctttatt
    10081 tcctgatagg caggtaaacc gagaactcgg tatgacctgg ccaactggta aattcaattt
    10141 tgcctgaatg ctgatcaatc aaattacggg cgatggataa gccaagcccg gtgccacctt
    10201 cgcggccgct gaccatcggg taaaacagcg tatcctgcaa atgaggcgga atgcccggcc
    10261 cgttatcttc cacatcaatc cgcgccgcca gccggtagcg ctcgccgtgt aaggtcagtt
    10321 gaaacgcggt gcgggtacgc agaatgattt caccgccttc cggccccagc gcctgtagcg
    10381 cattgcgcac aatattcagc aagacctgtt caatttgatc cgggtcgtgc gccagttccg
    10441 gcaggctggg atcgtaatca cgaatcaacc gcacgttgtc cggcagttcc atcgacacca
    10501 gcgttaccac gcgttcagcc actttgtgaa tactttcggt aacgcgcgta ccgggcagct
    10561 gcggccccaa cagacggtcg accagatttc gcagccggtc cgcctgttcg ataatcactt
    10621 tggtatattc gagtagtgat gggtcaggta acgctttgct gagcagctgc gccgcgccac
    10681 gtaaaccgcc aagcggattt ttaatctcat gtgccaggcc gcgcactaaa tcacgggcag
    10741 caacctgctg ggcgtgctgt agctgttcct gacttaagcg gcgctggtta tccatcggag
    10801 ccatctccag caggatcatg ccgtccggca tacgctgggc cgtcacagaa aggatatgcg
    10861 agcgcccgtc gatgaccagc gtcacttcgt tatcggtaaa accttgcccc gcctccagac
    10921 tttcttgcat cagctcgata tttaatgaga agtagctcaa cagttccggt aacggtgtac
    10981 caaacaattt gcgggagctt tgggcgagca gttgttgcgc ggcagggttg gcgtaatgga
    11041 tctccaggtt gtcatcgatt aacaaaatac tgttaatcag cgagttgagg atctgcccag
    11101 catcgggctg cgtgcctgtt gccataaagc agtctcctga acaggttgca ccattttagt
    11161 gcattatagc tttttacgga taaaaagcgc gaagcatcag agaattgacg gagaaaaaag
    11221 cccatgcaga gatgggctga aagtttccac ggcaactaaa tcccggcgtt gttgcgccgg
    11281 gtagtacaac ttcaactatt agacgctgta gtacagctca aactctaccg gatgcggagt
    11341 catacgcacg cggtcatctt cttcgcgacg cagagcgatg tacgcatcaa ttgcttcgtc
    11401 agtgaacacg ccaccggctt tcaggaactc gcggtccaga tccagttcgt tcagtgcttc
    11461 ttccagagag cctgcaacct gtgggatctc tttcgcttct tctggcggca ggtcatacag
    11521 gtttttgtcc atggcttcac ccggatggat cttgttcttg ataccatcaa gaccggccat
    11581 cagcagggca gcaaagcaca ggtacgggtt agctgccgga tccgggaaac gtacttcgat
    11641 acgacgtgct ttcggagaag aaaccaccgg aatacggata gacgcagaac ggttacgcgc
    11701 agagtaagcc agcattaccg gtgcttcata gcccgggacc agacgcttat aagagttggt
    11761 ggtcgggttt gccagggcgt taatcgcttt agcgtgtttg attacgccgc caatgtagta
    11821 cagcgcctgc tcagacagac ctgcgtattt gtcgcctgcg aacaggttaa cgccgttttt
    11881 agacagagac atgtggcagt gcataccgga gccgttatca ccgaacatcg gttttggcat
    11941 aaaggtcgcg gttttaccga agcggtgcgc tacgttgtgc acaacatatt tgtagatctg
    12001 aatttcgtca gcttttttgg tcatggtatt gaagcgggta gccacttcgt tctgaccagc
    12061 agtcgctact tcgtggtgat gggcttcaac caccagcccc atctgttcca tcaccagaca
    12121 catttcagaa cgaatatcct gagccgagtc taccggtgga accgggaagt aaccgccttt
    12181 cactgccgga cggtgacctt tgttaccacc ttcgtattgg gtggaggagt tccatgcgcc
    12241 ttcgatatcg tcgatagcaa cgtgggaacc ggagatagat gatccgaaac ggatgtcatc
    12301 gaacaggaag aattcaggtt ctggcccgaa cagtacggtg tcggcaatgc cagtggaacg
    12361 caggtaatct tcggcgcgct tcgcaatgga gcgcgggtca cggtcatagc cttgcagggt
    12421 gccaggttca aggatgtcgc aacggataat cagggtggag tcggcgaaga acgggtcaat
    12481 cactgcggtg gatgcgtctg gcatcagcac catgtcggac tcgttaatgc ctttccagcc
    12541 gccaatcgag gagccgtcaa acattttgcc ttcttcgaag aattcagcat tcacctgatg
    12601 agcagggata gtgacgtgct gttctttacc tttagtatcg gtgaagcgca aatcaacaaa
    12661 cttcacttcg tgctcgttca gcatcgtcag tacgtgttca gcggacatac tttaactctc
    12721 ctggattggt catggtcgtc gtggtaacga aatctgcaat tattttggcc gtgtcgccgt
    12781 aaaaaagata aagcgaaatc tgtgccaact tttaaattgc ccctaaaagg cgttatcatg
    12841 cgcaccatcg tgcaaaaggg ctgcaccacg atgtgaatgt tgcaccaata tagtgcttca
    12901 atggaaacat taagcaccat gttggtgcaa tgacctttgg ataacccttt ttatgctccg
    12961 tgaaagcgat cacaaaggga ctctgcaata cttgtttgcg gaggatgttt gtgatcctgt
    13021 tttgtagtgc gattaatccg tgtacaataa cgcgctattt ctaatgcctg aggcaaagtt
    13081 gtgatcgaaa aattgcgtaa tatcgccatc atcgcgcacg tagaccatgg taaaaccacc
    13141 ctggtagaca agctgctcca acaatccggt acgttcgact ctcgtgccga aacccaagag
    13201 cgcgtgatgg actccaacga tttggagaaa gagcgtggga ttaccatcct cgcgaaaaac
    13261 accgctatca aatggaatga ttaccgtatc aacatcgttg ataccccggg gcacgccgac
    13321 ttcggtggtg aagttgaacg tgtaatgtcc atggtagact cagtgctgct ggtggttgac
    13381 gcatttgacg gcccgatgcc gcaaacgcgc ttcgtaacca aaaaagcgtt tgcttacggc
    13441 ctgaagccga ttgttgttat caacaaagtt gaccgccctg gcgcgcgtcc tgattgggtt
    13501 gtggatcagg tattcgatct gttcgttaac ctcgacgcga ccgacgagca gctggacttc
    13561 ccgatcgttt acgcttctgc gctgaacggt atcgcgggtc tggaccacga agatatggcg
    13621 gaagacatga ccccgctgta ccaggcgatt gttgaccacg ttcctgcgcc ggacgttgac
    13681 cttgacggtc cgttccagat gcagatttct cagctcgatt acaacagcta tgttggcgtt
    13741 atcggcattg gccgcatcaa gcgcggtaaa gtgaagccga accagcaggt cactatcatc
    13801 gatagcgaag gcaaaacccg caacgcgaaa gtcggtaaag tgctgggcca cctcggtctg
    13861 gaacgtatcg aaaccgacct ggcggaagct ggcgatatcg ttgcgatcac gggccttggc
    13921 gaactgaaca tttctgacac cgtttgcgac acgcaaaacg ttgaagcgct gccggcactc
    13981 tccgttgatg agccgactgt ttctatgttc ttctgcgtta acacctcgcc gttctgcggt
    14041 aaagaaggta agttcgtaac gtctcgtcag atcctggatc gtctgaacaa agaactggta
    14101 cacaacgttg cgctgcgcgt agaagaaacc gaagacgctg atgcgttccg cgtttctggt
    14161 cgtggcgaac tacacctgtc tgttctgatc gaaaacatgc gtcgtgaagg tttcgaactg
    14221 gcggtatccc gtccgaaagt tatcttccgt gaaatcgacg gtcgtaaaca agagccatat
    14281 gaaaacgtga ctctggacgt tgaagaacag catcagggtt ctgtaatgca ggcgctgggc
    14341 gaacgtaaag gcgacctgaa aaacatgaat ccagacggta aaggccgcgt acgtctcgac
    14401 tacgtgatcc caagccgtgg tctgattggc ttccgttctg agttcatgac catgacttcc
    14461 ggtactggtc tgctgtactc caccttcagc cactacgacg acgtacgtcc gggtgaagtg
    14521 ggtcagcgtc agaacggcgt actgatctct aacggtcagg gtaaagcggt cgcgttcgcg
    14581 ctgttcggtc tgcaggatcg cggtaagctg ttcctcggtc acggtgcaga agtttacgaa
    14641 ggtcagatta tcggtattca tagccgctct aacgacctga ctgtaaactg cctgaccggt
    14701 aagaaactga ccaacatgcg tgcttccggt actgacgaag ccgttgttct ggttccgcct
    14761 atccgcatga ctctggaaca agctctggag ttcatcgatg atgacgaact ggtagaagtg
    14821 actccgacct ctatccgtat tcgtaaacgt cacctgacgg aaaacgatcg tcgtcgcgcc
    14881 aaccgcgcac cgaaagacta ttaatttcgt tttttagtta taaaaaacct gccatcgctg
//
LOCUS       DAJTNN010000096        14564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTNN010000096 DAJTNN010000000
VERSION     DAJTNN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.096"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..420
                     /locus_tag="OO150_002583"
     CDS             <1..420
                     /locus_tag="OO150_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5516681.1"
                     /translation="LMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRL
                     AEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTL
                     SIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            436..918
                     /locus_tag="OO150_002584"
     CDS             436..918
                     /locus_tag="OO150_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5516682.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(995..1342)
                     /gene="rplS"
                     /locus_tag="OO150_002585"
     CDS             complement(995..1342)
                     /gene="rplS"
                     /locus_tag="OO150_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5516683.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1384..2151)
                     /gene="trmD"
                     /locus_tag="OO150_002586"
     CDS             complement(1384..2151)
                     /gene="trmD"
                     /locus_tag="OO150_002586"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5516684.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2182..2730)
                     /gene="rimM"
                     /locus_tag="OO150_002587"
     CDS             complement(2182..2730)
                     /gene="rimM"
                     /locus_tag="OO150_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5516685.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEDYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2749..2997)
                     /gene="rpsP"
                     /locus_tag="OO150_002588"
     CDS             complement(2749..2997)
                     /gene="rpsP"
                     /locus_tag="OO150_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5516686.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3134..4495)
                     /gene="ffh"
                     /locus_tag="OO150_002589"
     CDS             complement(3134..4495)
                     /gene="ffh"
                     /locus_tag="OO150_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5516687.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4662..5453
                     /locus_tag="OO150_002590"
     CDS             4662..5453
                     /locus_tag="OO150_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5516688.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            5474..6760
                     /locus_tag="OO150_002591"
     CDS             5474..6760
                     /locus_tag="OO150_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5516689.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6815..7408)
                     /gene="grpE"
                     /locus_tag="OO150_002592"
     CDS             complement(6815..7408)
                     /gene="grpE"
                     /locus_tag="OO150_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5516690.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7531..8409
                     /gene="nadK"
                     /locus_tag="OO150_002593"
     CDS             7531..8409
                     /gene="nadK"
                     /locus_tag="OO150_002593"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5516691.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCKKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8495..10156
                     /gene="recN"
                     /locus_tag="OO150_002594"
     CDS             8495..10156
                     /gene="recN"
                     /locus_tag="OO150_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5516692.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10305..10646
                     /gene="bamE"
                     /locus_tag="OO150_002595"
     CDS             10305..10646
                     /gene="bamE"
                     /locus_tag="OO150_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5516693.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10708..10998)
                     /locus_tag="OO150_002596"
     CDS             complement(10708..10998)
                     /locus_tag="OO150_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5516694.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10988..11425)
                     /gene="ratA"
                     /locus_tag="OO150_002597"
     CDS             complement(10988..11425)
                     /gene="ratA"
                     /locus_tag="OO150_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5516695.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11596..12078
                     /gene="smpB"
                     /locus_tag="OO150_002598"
     CDS             11596..12078
                     /gene="smpB"
                     /locus_tag="OO150_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5516696.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12293..12655
                     /gene="ssrA"
                     /locus_tag="OO150_002599"
     tmRNA           12293..12655
                     /gene="ssrA"
                     /locus_tag="OO150_002599"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12816..14564)
                     /locus_tag="OO150_002600"
                     /pseudo
     CDS             complement(12816..14564)
                     /locus_tag="OO150_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         3590 a         3913 c         3431 g         3630 t
ORIGIN      
        1 ttgatgaaca attccgatgc ccgaaaaacg tcggcgttac tatttcttga tggcgataat
       61 ttcaaatata tcaatgatac ctggggtcat gcgacgggcg atagggtctt gattgaaatc
      121 gcaaaacggt tagctgaatt tggcgggctg cgacataaag cataccgcct gggcggcgat
      181 gaattcgcta tggtgctcta tgatgtacag tcggaatctg aagtgcaaca gatatgctca
      241 gcactgacac aaatctttaa tctcccgttt gatcttcata atggccatca gaccaccatg
      301 acattaagca ttggttacgc gatgaccatt gagcacgcct ctgcggaaaa attacaagag
      361 cttgccgatc acaatatgta tcaggccaaa caccagcgtg ccgaaaagct ggtgagataa
      421 caaggatata tcgatatgat aaagcacctg gtagcccccc tgattttcac ctcactaata
      481 ctgactggct gccagtcccc tcagggaaag tttactcctg agcaagtcgc cgctatgcaa
      541 tcttatggat ttactgaatc cgccggcgac tggtcgctgg gcttatcaga tgccattctg
      601 ttcgcaaaaa atgactacaa attgctcccg gaaagccagc aacagatcca aaccatggca
      661 gccaaattgg cctcgacagg gctaacacat gcccgtatgg atggacacac cgataactat
      721 ggtgaagaca gttacaacga aggcttatca ttgaaacggg cgaatgtcgt ggccgatgca
      781 tgggctattg gtggacaaat tccacgcagc aatctcacca cacagggttt aggaaaaaaa
      841 tatcccatag ccagtaacaa gaccgcccag ggccgcgccg agaaccgccg cgtcgcagtg
      901 gtgattacaa ccccttaaaa cacgagcaaa cagacaaaaa aagggccagc caattggcca
      961 gcccttctta acaggatgtc gcttaagcga aatcttagtt aagacgctct ttgatacgag
     1021 cagccttacc agtacgctca cgcaggtagt acagtttagc tttacgaaca gcaccacgac
     1081 gtttgacaga aatgctgtca actaccggag agtgagtctg gaagacacgc tcaacgcctt
     1141 cgccgttgga aattttacga acagtgaatg cagagtgcag accgcggtta cgaatagcga
     1201 taaccacgcc ctcgaatgcc tgcagacgtt ttttggaacc ttcaacaacc catactttca
     1261 cttccacggt atcacccgga cggaaggaag gtacgtcctg cttcatctgc tcttgttcaa
     1321 gttgcttaat aatgttgctc ataatttaat ctcttatcct gggtaaactg atatctcggg
     1381 ggcttacgcc atcccatcat gtttatgttg ctgttgtgcg tgttccgttt tgaactccgc
     1441 cagcaacctt gcttgctctt cagtcagagc caggttttcc agaagttcag gtcttctaag
     1501 ccaggtacgg cccagcgact gtttcaaacg ccagcgacgt atctcggcat ggttgcccga
     1561 cagtaacact ggcggaactt ccatcccttc taacacctca ggccgcgtat agtgcgggca
     1621 atccagcaat ccttcagcaa aggaatcttc cgttgccgag gcttcatgtc ccagtacccc
     1681 cggaataaac cgggaaacgg agtcaatcag cgtcattgct ggtaactcac caccactgag
     1741 aacgtaatcg ccgattgacc attcttcgtc aatttcggtt tggatcacgc gctcatctat
     1801 accttcgtag cgaccgcaca ccagaatcaa tttttgattc gttgccaatt cgctgacgcc
     1861 cgcttgatca agcttgcgtc cctgtggtga cagataaatc acctttgcgc cttcacccgc
     1921 cgcggctttt gctgcatgaa tggcgtcccg caagggttgc accatcatta acatccccgg
     1981 tccgccgccg taaggacgat cgtccacggt acggtgccgg tcatgcgtga agtcgcgagg
     2041 actccagctc tggatgctca gcaggccatt tttaactgcc cggccagtta ccccgtaatc
     2101 ggtaattgcg cggaacattt caggaaacag gctaattatg ccaatccaca tagcgccgtc
     2161 ttttaccgtt tatccggtgg tttaaaaacc aggatcccaa tctacttcga ttgaacgagt
     2221 agtgagatcg actttcttga taacctgccc atcgaggaac ggtacgagac gttccttgat
     2281 accaaacgca tctttcaggt ttgccttaat gacgagaacg tcattagatc cggtttccat
     2341 catatcgacg actttaccga gatcgtagtc ttcagtggtt actacctggc agcccatcag
     2401 gtctttccag tagtagtcac cctcttcaag ctgaggcaac tgcgatgaat ccacgacaat
     2461 ttcacaattc gtcagcaggt tcgccgcatc acgatcgtca acgcctttca gcttgatgat
     2521 catgtcctga ttgtggtgct tccagctttc cagctggact tgctgccact gacccgcctt
     2581 ctggataaac cagggctgat agtcaaaaat gctttcggcg tcttcggtgg aagaaaacac
     2641 tctgagccac ccacgaatac cgtaagacga acccattttt cccaaaacga tgggatcaac
     2701 aggtgcttgc gcggtgagtt gtttgctcat catgaccacc gtgacagatt aagctgcttt
     2761 gtttacttct ttgatcagcg cagcaacgcg atcagaaata gttgcgccct ggccaaccca
     2821 gtgagcgatg cgatccagat ccaggcgagt gccttcttct ttttcgctag cgattgggtt
     2881 gaagaaacca acgcgctcga tgaagcgacc gttgcgtgca ttacggctgt cagcgacaac
     2941 aacctggtag aacggacgct ttttagcgcc gtgacgtgct aaacgaatag ttaccataac
     3001 atcctcttgt gtgaataaaa caaccggacc ccatcgagga acggagtccg gtgtcatatt
     3061 aaaagcccga aaattttact gatttctgga gaaaatgcaa tcagcagtta ataactctgt
     3121 cgcataaggt catttagcga ccaggaaatc ccggcggcat catccccttc atgcttctca
     3181 tcatcttcgc cattccgccc ttcttcattt tcttcatcat gcgctgcatg tcgtcgaact
     3241 gtttcagaag acggttaacg tcctgcacct gcataccgca accggcagca atacggcgtt
     3301 tacgcgaacc tttgatgatt tctggcttag cgcgctcttt catcgtcatc gagttgatga
     3361 tggcttccat acgcaccagc actttatcgt ccatctgtga cttgacgtta tccgggatct
     3421 gccccatgcc cggcagcttg cccatcaggc tggccatgcc gcccatattt ttcatctggc
     3481 gcagctgctc aagaaagtcg ttgagatcga agccgtcacc ttttttcagc ttgctggcta
     3541 atttctctgc ctgcgcgcgg tcaactttgc tttcgatatc ttcgatcagc gacagtacgt
     3601 cgcccatgcc gagaatacgc gacgcgatgc ggtccggatg gaacggctcc agcgcctcag
     3661 ttttctcgcc aacaccgagg aacttgatcg gtttgccagt gatgtgacga atagagagcg
     3721 ccgcaccgcc gcgggcatcg ccgtccactt tggtcaacac tacgccggta agcggtaacg
     3781 cttcattgaa tgcttttgcc gtattggccg catcctgacc ggtcatggcg tcaaccacaa
     3841 acagggtttc aaccgggtta atcgacgcat ggacttgttt gatctcgtcc atcatcgctt
     3901 cgtcaacgtg cagacgacca gcggtatcca ccagcagcac gtcgtagaat ttcagtttgg
     3961 cttctttcag cgccgcgtta acgatatcta ccggcttctg accaacatca gaagggaaga
     4021 aatcaacgcc cacctgctct gccagcgtct caagctgttt gattgccgcc gggcgataaa
     4081 cgtcggcaga aaccaccagc actttcttct tgtgcttctc gcgcaggaac ttaccgagtt
     4141 taccaacgct ggttgtttta ccggcacctt gcaggcccgc catcagcacg accgcaggcg
     4201 gttgcgccgc caggttcagg gtctggttct cttcgcccat cgccgcaacc agttcgttac
     4261 ggacgatttt gacgaactcc tgccccggcg tcaggctctt attaacttca tgaccaaccg
     4321 ctttctcttt tacgcgattg ataaactcac gcactaccgg cagagcgaca tccgcctcca
     4381 gcagcgccat gcgcacttcg cgcagcgtat cttttacgtt gtcttcagtt aggcgtccac
     4441 ggccactgat attgcgcagc gtgcgcgaca aacgatcggt taaattatca aacattgtct
     4501 ctcgcctggg gtggaaacgg ttggccgcaa tcgcgacacc tcatcagtat tttgccgcag
     4561 tataacatga aggcgtcttt gttgttatgc aacggttgga gcagcgttca cctgacgcta
     4621 tactgcttct ctttcttatt gctcaaactg ttgacatcac tatgcccgtt tttgctctgc
     4681 tcgcgcttgt cgcctactcc gtcagtcttg cgctgattgt tcccggtctg ctgcaaaaaa
     4741 acggcggctg gcggcgcatg gctattattt ctgcggtcat tgcgctggtc tgccacgcaa
     4801 tcgctctgga agcccgcatc ctgcccgacg gtgatagcgg acaaaacctc agcctgctga
     4861 acgttggttc attggtcagt ttgatgatct gtacggtaat gaccattgtg gcttctcgca
     4921 atcgtggctg gttgctgcta cccattgtct atgcctttgc gcttatcaac ctggcgctgg
     4981 cgaccttcat gcccaatgaa tacatcaccc atctggaagc aacgcctggg atgctggtgc
     5041 acattggctt atcgctcttt tcctatgcca cgctaattat cgccgccctg tacgcgctgc
     5101 aactggcgtg gattgattac caactgaaga acaagaagct ggcatttaac caggaaatgc
     5161 cgccattgat gagtatcgag cgtaaaatgt tccacatcac gcagattggc gtggtgctgc
     5221 taacgctcac gctttgcact ggcctgttct acatgcacaa cctatttagc atggaaaata
     5281 tcgacaaggc tgtgctctct atcgtggcgt ggtttgtcta tattgtgctg ctgtggggac
     5341 attaccatga aggatggcgt ggacgccgcg tcgtctggtt taacgttgcg ggcgcggtaa
     5401 ttctgacact ggcctacttc ggcagccgaa ttgtccagca gttaatcagc taaacccaga
     5461 aaaggagttt cccctggaac acatttctac tactacgttg atcattattc tgatcatcat
     5521 ggtggtcatt tcagcctatt tttccgggtc cgaaaccgga atgatgaccc tcaaccgcta
     5581 tcgtctgcga catatggcga aacagggtaa tcgctcggcc aaacgcgtcg aaaaattgct
     5641 gcgtaagcca gaccgcctga taagcctggt gttaatcggc aataacctgg tcaatattct
     5701 tgcctccgcg ctcggcacta ttgttgggat gcgtttgtac ggcgatgcgg gcgtggcaat
     5761 tgcgactggt gtgctgactt ttgtcgtact ggtatttgct gaggtattgc cgaaaaccat
     5821 tgccgcgctg tacccggaaa aagtcgctta tccgagtagt tttctgctgg ctccgctgca
     5881 aattttgatg atgccgctgg tctggttgct gaatgctatc acccgtatgc tgatgcgcat
     5941 gatgggtatc aaaaccgata tcgtggttag cggctctttg agcaaagaag agttgcgcac
     6001 tatcgtgcac gaatcacgct cacaaatttc ccgtcgcaat caggatatgc tgctgtcggt
     6061 gctcgatctg gaaaaaatga ccgttgatga catcatggtg ccgcgcagtg aaattatcgg
     6121 tattgatatc aacgatgact ggaaatcgat tctgcgccaa ctctcccact cacctcacgg
     6181 gcgcatcgtg ctctaccgtg attcgctgga cgacgccatc agtatgctgc gagtacgtga
     6241 agcctggcga ttgatgtcgg agaaaaaaga gttcaccaaa gaaaccatgc tgcgcgccgc
     6301 ggacgagatc tattttgtgc cggaaggtac gccgctcagc acgcagttgg taaagtttca
     6361 gcgcaacaaa aagaaagtcg gcctggtcgt caacgagtat ggagacattc aggggctggt
     6421 gacggttgaa gatattctgg aagagattgt cggcgatttc actacgtcga tgtcgccaac
     6481 acttgccgaa gaggtcacgc cgcaaaacga cggttcggtg attatcgatg gcaccgccaa
     6541 cgtgcgggaa atcaacaaag cctttaactg gcatctaccg gaagatgatg cccgcacggt
     6601 taatggcgtc attcttgagg cactggaaga gatccctgtc gcaggcaccc gcgtgcgtat
     6661 tggcgagtac gatatagata ttctcgacgt acaggacaat atgattaagc aggtaaaagt
     6721 ttttcctgtg aaaccgctgc gcgagagtgt ggcggagtaa cgaaaacggc cctgcattcg
     6781 catgcagggc cgtgaattat tacgaaaaca gaaattacgc ttttgctttc gctacagtta
     6841 ccatcgccgc acgaatcgta cgaccattca gcgtataacc cttctgcata atgcccagta
     6901 cgttacctgg cgcaacgtca tcagattcca ccattgcgat agcctgatgc acattcgggt
     6961 ccagcggcac gttagtttcg gcgatcactt caacaccaaa cttacgcaca acatccagca
     7021 tcgacttcag cgtcagctca atgccttcaa ccatcgcaga catatccggg ttagctttat
     7081 cagccacttc cagcgcacga tccaggctat caatcaccgg cagcaattcg ttgatgaatt
     7141 tctccagcgc aaatttgtgg gctttttcaa tatccagttc agtacgtcga cgcaggtttt
     7201 ccatttcggc ttttacacgc aaaatgccgt cacgttcacg ggtctgggct tcagccagct
     7261 gagcttcgag attcgcaatt ttttcatcgc gcggatccac ctgctcagca gaagcttctg
     7321 gctcaactgc ctcaatctct tcgtgctgat ccatgataat ttcttccggg gcttgcccct
     7381 caggcgtttt ctgttcttta ctactcatga atttctccgc gtttttttcg cattcatctc
     7441 gctaacttcg cttattatgg ggatcagttt cagggtttca agggaagcac tcacattgtc
     7501 atcaatcttc gcaacaagga cctcggaaaa atgaataatc atttcaagtg tattggcatt
     7561 gtgggacacc cacggcaccc cactgcactg acaacacatg aaatgctcta ccgctggctg
     7621 tgcaaaaaag gttacgaggt catcgttgag caacaaatcg ctcacgaact gcaactgaag
     7681 aatgtgaaaa ctggcacgct cgcggagatt gggcaactag ctgatctcgc ggtagtcgtt
     7741 ggtggcgacg gtaatatgct gggcgcggca cgcacactcg cccgttacga tattaaagtt
     7801 attggaatca accgtggcaa cctgggtttc ctgactgacc ttgaccccga taacgcccag
     7861 caacagttag ccgatgtact ggaaggccac tacatcagcg agaaacgttt tttgctggaa
     7921 gcgcaagtct gtcagcaaga ttgccagaaa cgcatcagca ccgcgataaa tgaagtggtg
     7981 cttcatccag gcaaagtggc acatatgatt gagttcgaag tgtatatcga cgagatcttt
     8041 gcgttttctc agcgatctga tggactaatt atttcgacgc caacaggctc caccgcctat
     8101 tccctctctg caggcggtcc tattctgacc ccctctctgg atgcgattac cctggtgccc
     8161 atgttcccgc atacgttgtc agcacgacca ctggtcataa acagcagcag cacgatccgt
     8221 ctgcgttttt cgcatcgccg taacgacctg gaaatcagtt gcgacagcca gatagcactg
     8281 ccgattcagg aaggtgaaga tgtcctgatt cgtcgctgtg attaccatct gaatctgatt
     8341 catccgaaag attacagtta tttcaacaca ttaagcacca agctcggctg gtcaaaaaaa
     8401 ttattctaat tttacgccag cctctttact gtatataaaa ccagtttata ctgtacgcaa
     8461 taacagtaat ggtttttcat acaggaaaac gactatgttg gcacaactga ccatcagcaa
     8521 ctttgctatc gttcgtgagc ttgagattga ttttcatagc ggcatgaccg taataactgg
     8581 cgagaccggc gcgggtaaat ctattgcaat agatgccctc ggtctttgtc tcggtggtcg
     8641 cgctgaagcc gacatggtgc gtaccggcgc tgctcgcgct gacctgtgcg cccgtttttc
     8701 tctgaaagat acgccagcgg ctctgcgctg gctggaagaa aatcagcttg aagacgggca
     8761 tgaatgtttg cttcgtcgcg taatcagcag cgatggtcgc tcccgtggtt tcatcaacgg
     8821 tacagctgtt cctctgtcac aactgcgcga actgggtcag ttgctgattc agatccatgg
     8881 tcagcacgct catcaattac tcaccaaacc tgagcaccaa aaattcctgc ttgatggcta
     8941 tgccaatgaa acctctctac tgcaggaaat gaccgcacgt tatcagttgt ggcatcaaag
     9001 ctgccgtgac ctcgcgcatc atcaacagtt aagtcaggaa cgcgccgccc gtgcggaact
     9061 gctgcaatac caattaaaag aacttaacga atttaatccg cagcccggag agtttgaaca
     9121 aatcgacgaa gagtacaaac gtctggcgaa cagcggtcaa ttgctgacca ccagccagaa
     9181 tgcattggca ttaatggcct acggtgaaga cgcaaacctg caaagtcagc tttacacggc
     9241 taaacaactg gtgagcgaat tgattggcat ggacagcaaa ctgtccggcg tacttgatat
     9301 gctggaagaa gctaccatcc agattgctga agccagcgat gaactgcgcc actactgcga
     9361 tcgtctggat ctcgatccca accgactatt tgaacttgaa cagcgcatct caaaacagat
     9421 ttcgctggca cgtaaacatc acgtcagccc tgaggcattg ccacagtatt accagtcgct
     9481 actggaagaa cagcagcaac tggacgatca ggccgactca caagaaacgc ttgcgctggc
     9541 ggtaacgaaa catcatcagc aggcactgga aatcgcgcgc gcattacacc aacaacgcca
     9601 gcaatatgca gaagaacttg cacagctgat caccgacagt atgcatgcgc tctcaatgcc
     9661 gcatgggcag tttacgatcg atgttaaatt tgacgagcat cacctgggcg ctgacggtgc
     9721 cgatcgtatt gagtttcggg taaccaccaa cccaggtcag ccaatgcagc ctattgccaa
     9781 agtcgcatcc ggtggtgaat tgtcccgcat cgcactggca acccaggtca tcacggcgcg
     9841 taaaatggaa accccggcac tgatttttga tgaagtggat gtagggatta gcggtccaac
     9901 agcggcagtt gtcggcaaac tgctgcgtca actcggcgaa tcaactcagg tgatgtgtgt
     9961 tacccacctg ccacaagtcg cgggatgtgg tcatcaacac tattttgtca gcaaagaaac
    10021 cgatggtgcg atgacagaaa cgcatatgca atccctgaat aaaaaagcgc ggttacaaga
    10081 gctggcgcgc ctgcttggtg gcagtgaagt cacacgtaat acactggcga atgcgaaaga
    10141 actgcttgca gcgtaaactt ttttcctgct tcacggtcag agtaaacagc aaaacgccgt
    10201 aagaccggaa agcaaaaggt tttaaagtga tgaaaggtct attatcatcg gcatattaca
    10261 gatgagccac gtactgctag ggcccgaaaa ggaatcaaat cactatgcgc tgtaaaacgc
    10321 tgactgctgc agcagcagta ctattgatgt tgaccgcagg ctgttccact ctggagcgag
    10381 tggtttaccg tcctgacatc aaccagggga actatctgac cgctaacgac gtatccaaaa
    10441 tacgtgttgg catgacgcaa caacaagttg cgtacgcatt gggtacaccg ctgatgtccg
    10501 atccatttgg tacgaatacc tggttctatg tcttccgcca gcaaccaggt catgaaggtg
    10561 taacgcagca aacgctgacg ctgaccttta acagtagcgg tgtgttgacc aatattgata
    10621 acaaacctgc gctgagtggt aactaataat aaagttgctc tcaaagacgt taaaaaaggt
    10681 gctcaatgag cacctttttt ctgtctgtta tttattcgct gatttttctg ctcgttgcct
    10741 gcgaagctct ttcggatcgg caatgagagg gcgataaatc tccacccgat cgccatcatg
    10801 cacactatcg cttagttttg ccggacggct gtaaatgccg actttgtttt tagttaaatc
    10861 gatatcggta cgcaattcca gcaagccact ggcgcgaata gcttcttcaa ccgtcgcgcc
    10921 ctcctgcagc gtcacgcgct gcaggtactg cttctcaggt agcgcataag ccacctcaac
    10981 ggcaatttta cctggcactg taaacctctt tcgcacgaac cgtaaaagcc tggaccatat
    11041 tagacgccag ctctttaaac acgcgaccaa aggcgagttc aatcaactta ttggtaaact
    11101 caaagtcgag atgaaactcg atacgacacg cctcctggct cagcggcgta aacttccatc
    11161 caccaatcaa tttcttgaac ggcccatcca ccagattcat aagaatactt tggttactgg
    11221 tcaactggtt gcgggtagta aacgttttgc tgatcccagc cttagagaca tctaccgccg
    11281 cagtcatctg cccaggagtg gactccagaa tccgacttcc ggtacaaccc ggcaaaaact
    11341 gaggataaga ctgaacgtca ttcactaact gatacatttg ctccgcgctg tagggtacca
    11401 gtgcggtccg gctaatctga ggcataacta tttccatcaa caaaaatcca acaaataata
    11461 tcatttatcc tgctgtaaaa aaaacgctat cccggcgctg ggtaacatcg ggttcatgct
    11521 aagatagagc cttgtccccc gcaggattga tatggggtgt tttcgatttc agattaccga
    11581 tgattcacga cgcttatgac gaagaaaaaa gcacataaac ctggttcagc gaccatcgcg
    11641 cttaacaagc gcgcccgtca cgaatacttt atcgaagaag agttcgaagc gggacttgcc
    11701 ctgcaaggct gggaagttaa atccctgcgc gcaggaaaag ccaatatcag cgacagctac
    11761 gtccttctgc gtgacggaga ggcatttctg tttggcgcta acatcacgcc aatggccgtg
    11821 gcctccacgc atgtggtgtg cgatcctacc cgtacccgca agttacttct caaccagcgc
    11881 gaactggact cattgtacgg tcgcgtcaat cgagaaggct ataccgtagt ggcgctctcc
    11941 ctgtactgga aaaatgcctg gtgcaaagtg aaaatcggcg tcgccaaagg taaaaaacag
    12001 cacgataaac gttcagatat caaagaacgc gaatggcagg tggataaagc acgtatcatg
    12061 aaaaacgccc accgttaaac ctgcactcca attattgacc agttcctcac cgcgcctccc
    12121 tctccggcgg cgcgaatgaa catcttattg gctatcacat ccgacacaaa tgttgccatc
    12181 ccattgctta atcgaataaa aatcaggcta catgggtgct aaatctttaa cgataacgcc
    12241 attgaggctg gtcatggcgc tcataaatct ggtatacttg ccgttacaca ttggggctga
    12301 ttctggattc gacgggattt gcgaaaccca aggtgcatgc cgaggggcgg ttggcctcgt
    12361 aaaaagccgc aaaaaatagt cgcaaacgac gaaaactacg ctttagcagc ttaataacct
    12421 gcttagagcc ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc
    12481 caaaagagat cgcgtggaag ccctgcctgg ggttgaagcg ttaaaactta atcaggctag
    12541 tttgttagtg gcgtgtccgt ccgcagctgg caagcgaatg taaagactga ctaagcatgt
    12601 agtaccgagg acgtaggaat ttcggacgcg ggttcaactc ccgccagctc caccaatcat
    12661 gattggacgg tataaggaca acaccaataa aaacaggagg ttagcagtct cagcaggaca
    12721 ccgaccagac ggtgaagaga cataaaagga tacgcaaagg agccgcggct cctggtgaca
    12781 tgaaagccca cagatgtggg ctttttcgtt gatggtcaga acgaccagtt cacaccagct
    12841 accccgttcc acggggattc cacaccggca ccatggctat accccacccc aagatgcccg
    12901 cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg tctgcccgac
    12961 aaatcattga cgaaattacc gtcactattc actttcaccc ggttatcatc gacaaattct
    13021 ttgcgcacag ccgccttcag ccacggctca acttccatac cgttccccag acgcatgttg
    13081 taactcagcg ttgcacccag ttcacgatat aaacctgcgg gtatcgactg atttcgattc
    13141 catgccattg gataaatgat attcagggtt atcagcggtg accccccgtt aacgatgcat
    13201 acggcgtcag gttccagtta ccatcggtaa atcgcatccc ggtttcaatg tgaccgccca
    13261 gcccgttgct gcgataactg ccgttggtgg ctccaccgct gccaaccgtg gagtcgcgag
    13321 cttcggtacc tgctaatacc aataaattac cgccattttc cagcaacata ttggtcgcta
    13381 aattgccgga aatggaaaaa gtgccgtact ggtaagtacc gctgatttca ataccgttag
    13441 ccgtgctcgt ctggagagcg gcaccgctgt tctggacgat atctgtcgct ttgccattat
    13501 cgttaactgt cagcgtaccg ccttcattga tctttgtttt tattgcctct ccgttagctg
    13561 aaactgtttg tattccgccg tcgttaatcg ttgtctcatt cgccaccccc tcgacgattt
    13621 gttcgccgcc ggtgagcgtc gtgcctgtcg cagtggcttt tgttttgacg atctcccgtc
    13681 cgcccgtatt gacctgtgtt ttgtcagaag aggtgtctga ctccacggtt aacacgccgc
    13741 catttgccag caggatattg ttcgccgcac cctgctcgat gctgaacgcg acgccatccg
    13801 cgcgtgttcc tgtgacccgc gtcgccctgg tggttgcaac caaagcgccc tggctactct
    13861 gctgtatccc cgttgcgctg cctttctccc gcacatcgag tgtgccgccg tcattaagca
    13921 ccgagttttc agccagaccg ccctcattaa ctacctgtga acccccatta ataatggaac
    13981 cttccgctgt cccgtttgcc ataatttgtt gtaggccaga gacgatatcg gtattgattg
    14041 ccttaccata attctgaacg gtttgcgtgc caccattgat gtgtgttttc tctgttgacc
    14101 caccatcaac aatttgttca ccactttcga tatttgcctc agtggctaat ccatataccg
    14161 tttgcttgcc acctttgata tttgctttat cagaagtggc actggcatat attgtttggg
    14221 tgccagcact attgagtaca gtgccaacat cttttccata aacatccatt ttgccgttgg
    14281 cattaataat cgtatcaact gcccgggaac cagtgacgac tgttaatgag ccagcgtttt
    14341 ccagcactac atttttagct tctgaattcc tgatgtagaa agcatcacca taactgttgg
    14401 ttcctttgat aagggttccg gaagttgtgg aagcaattaa tgcgccgccg gattgttgct
    14461 caacatgctt agcctcacca ccgtcctgaa cctccagaac gccaccatta ttaagtctgg
    14521 tggtatctgt tttagcctcc ttctggacaa tcagcttacc gcca
//
LOCUS       DAJTNN010000097        14563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTNN010000097 DAJTNN010000000
VERSION     DAJTNN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.097"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO150_002601"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO150_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5516697.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO150_002602"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO150_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5516698.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO150_002603"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO150_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5516699.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO150_002604"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO150_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5516700.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO150_002605"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO150_002605"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5516701.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO150_002606"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO150_002606"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5516702.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO150_002607"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO150_002607"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5516703.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO150_002608"
     CDS             complement(4598..5320)
                     /locus_tag="OO150_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5516704.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO150_002609"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO150_002609"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5516705.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO150_002610"
     CDS             6520..6738
                     /locus_tag="OO150_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5516706.1"
                     /translation="MQDLSLEARLAELESRLAFQESTIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO150_002611"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO150_002611"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5516707.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO150_002612"
     CDS             complement(7472..7672)
                     /locus_tag="OO150_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5516708.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OO150_002613"
     CDS             complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OO150_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR5516709.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEE
                     DEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYY
                     GDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHE
                     LLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHA
                     DTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO150_002614"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO150_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5516710.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OO150_002615"
     CDS             10175..12088
                     /locus_tag="OO150_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5516711.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OO150_002616"
     CDS             12088..13110
                     /locus_tag="OO150_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5516712.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OO150_002617"
     CDS             13104..13322
                     /locus_tag="OO150_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5516713.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OO150_002618"
     CDS             13376..14245
                     /locus_tag="OO150_002618"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5516714.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..>14563)
                     /locus_tag="OO150_002619"
     CDS             complement(14300..>14563)
                     /locus_tag="OO150_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5516715.1"
                     /translation="SAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIV
                     TGRDLKDAAVARAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
BASE COUNT         3599 a         3970 c         3587 g         3407 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtctag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaagcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggcc cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ttcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ctgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccggcgt tatcgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc aaggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcaacaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcaag cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gcgacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtgatatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc cctcgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atattatgcc gcgcagtcag tttcgcgaat
    13321 aacctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga agcgaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg aacattcacc ccctggcaac ctttaccaga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtagtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctg gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcggc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atcgaaacca catcgatggc act
//
LOCUS       DAJTNN010000098        14528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTNN010000098 DAJTNN010000000
VERSION     DAJTNN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.098"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1264)
                     /locus_tag="OO150_002620"
     CDS             complement(<1..1264)
                     /locus_tag="OO150_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR5516716.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQVAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPAARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNAPNFATTMTNALAGKQPKDATLTALAGLATAADRF
                     PYFTGNDVASLATLTKVGRDILAKSTVAA"
     gene            complement(1267..1797)
                     /locus_tag="OO150_002621"
     CDS             complement(1267..1797)
                     /locus_tag="OO150_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCR5516717.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            complement(1790..2686)
                     /locus_tag="OO150_002622"
     CDS             complement(1790..2686)
                     /locus_tag="OO150_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5516718.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCASFTISEGGRNE"
     gene            complement(2690..3019)
                     /locus_tag="OO150_002623"
     CDS             complement(2690..3019)
                     /locus_tag="OO150_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCR5516719.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            complement(3037..3603)
                     /locus_tag="OO150_002624"
     CDS             complement(3037..3603)
                     /locus_tag="OO150_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR5516720.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTSRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(3615..4250)
                     /locus_tag="OO150_002625"
     CDS             complement(3615..4250)
                     /locus_tag="OO150_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR5516721.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     PQKAPDGTAWTPRRRHVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNS
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(4243..4710)
                     /locus_tag="OO150_002626"
     CDS             complement(4243..4710)
                     /locus_tag="OO150_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5516722.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLLEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(4848..5255)
                     /locus_tag="OO150_002627"
     CDS             complement(4848..5255)
                     /locus_tag="OO150_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCR5516723.1"
                     /translation="MKLWPTLGVAFLLTAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(5252..5644)
                     /locus_tag="OO150_002628"
     CDS             complement(5252..5644)
                     /locus_tag="OO150_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR5516724.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQMSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(5641..5964)
                     /locus_tag="OO150_002629"
     CDS             complement(5641..5964)
                     /locus_tag="OO150_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5516725.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(5967..6167)
                     /locus_tag="OO150_002630"
     CDS             complement(5967..6167)
                     /locus_tag="OO150_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCR5516726.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(6167..6661)
                     /locus_tag="OO150_002631"
     CDS             complement(6167..6661)
                     /locus_tag="OO150_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCR5516727.1"
                     /translation="MTIVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(6829..7575)
                     /locus_tag="OO150_002632"
     CDS             complement(6829..7575)
                     /locus_tag="OO150_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5516728.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKKQQK"
     gene            complement(7621..8493)
                     /locus_tag="OO150_002633"
     CDS             complement(7621..8493)
                     /locus_tag="OO150_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCR5516729.1"
                     /translation="MIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLKNR
                     KYQCEQVNYDTFISYPQLDAWSAHPDFQSRISAQIARQVALDRIMIGFNGTSHADESN
                     FSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQDAR
                     SSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIGGL
                     GVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVEDY
                     GKCAMVTGLKFADS"
     gene            complement(8490..8672)
                     /locus_tag="OO150_002634"
     CDS             complement(8490..8672)
                     /locus_tag="OO150_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCR5516730.1"
                     /translation="MNLVMSDITRNKLGCYMAQQVSLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKKIT"
     gene            complement(8696..9532)
                     /locus_tag="OO150_002635"
                     /pseudo
     CDS             complement(8696..9532)
                     /locus_tag="OO150_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
     gene            9687..11438
                     /locus_tag="OO150_002636"
     CDS             9687..11438
                     /locus_tag="OO150_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCR5516731.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPGQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPSVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            11438..12474
                     /locus_tag="OO150_002637"
                     /pseudo
     CDS             11438..12474
                     /locus_tag="OO150_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(12953..13543)
                     /locus_tag="OO150_002638"
     CDS             complement(12953..13543)
                     /locus_tag="OO150_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5516732.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADKHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            complement(13607..13918)
                     /locus_tag="OO150_002639"
     CDS             complement(13607..13918)
                     /locus_tag="OO150_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5516733.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(13923..>14528)
                     /locus_tag="OO150_002640"
     CDS             complement(13923..>14528)
                     /locus_tag="OO150_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR5516734.1"
                     /translation="NRKKANVMRPVEYQNGEAFTIRNVQVMPESIPAGFKALADMSPF
                     ESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSHHI
                     ANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVMVV
                     GGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
BASE COUNT         2950 a         4047 c         4005 g         3526 t
ORIGIN      
        1 cggcagcaac ggtcgatttc gcaagaatat cccgcccgac ttttgtcagg gttgccaggc
       61 tggcgacatc attccccgta aaatacggaa acctgtctgc cgcagtagca agcccggcca
      121 gcgccgtcag ggtggcatct ttcggttgct tacccgcaag cgcgttagtc atggtggtcg
      181 caaaattcgg ggcgtttccc agcgccgccg ctaactcgtt cagcgtattc agtgcgtcag
      241 gtgacgaatc tacaagggcg gcaatcgcag ccataacgaa agccgtgctt gcgatttggg
      301 tattattcgt tccctgtcgc gcagctggcg ttgttggcgt tccggtcagc gcaggactgt
      361 ttaagggcgc tttcttgttc gtttcatcca ttaccgcctt aaccgctttc gatgtcgcgg
      421 ccagtgtttc agacgtgctg ttggtggcac tactgagctg gactatccct ttttgtgccg
      481 tcgtggcgtc ctgagcggta tattttgcgt tagcaaggtc atacgcggcc tttactgctt
      541 tcggcgttgc agcctgcgtt tcagacgtgc tgttggtggc gctactgagc tggacaaggc
      601 cttttcgcgt cgtgctggca tcctgcgcag tatatttacc gttagcaaga tcatacgcgg
      661 cctttactgc tttcggcgtt gcagcctgcg tttcagacac gctgttagtg gcgctactga
      721 gttgaacaaa gccttttgcg gtcagcgagg cgtccgggtg acgtcgtgac tgttcatgct
      781 ctttcagttt gtcatccacg taatccactg tggccatcac catggtgttg tccacggtaa
      841 gcgccacggt ggcagtgctg gatacggtca gaatggtgcg aaatgtttgt gcacgaccgg
      901 acccttcggc aacggctggc ttgtaacttt cggcagtatt tcccaccgcg attaaatcgc
      961 cgtgctcatc aaatacacca atttcccgga tccagaatcc gcccgtttca ggaggaataa
     1021 ccagctccgc aataatgcgg ttctgatgtg ttgcgtccag gatgacgcga ttaacagtat
     1081 gtcgccacac ctcatgcacc agacgggtct gcttactgtc tggtgtgggc aacgtgccgc
     1141 caccgtcgcc cacggccata tgagtcaggc ggacaggctt accatctggc gcggctacct
     1201 gagctaattt ttttgcaccc gtatcggtga taatggtttt aaattttcgt gttgtggtac
     1261 tcatgcttaa tcgcctggat aaatggtaat aacttcaccg tcataagttg ccgccgctgc
     1321 gaaaatatcc cccggaattt cctgaatgat attcagccct gtcatgtggc ggctgaccgg
     1381 gcgggcatca gcaatcaacc gctccatttc cagatacatt tcctccgtca cgccactgtc
     1441 cagcgtgccg acttcaacgg taaatgttcc cggttctccg ccgaactccc accactcaga
     1501 cacgcgaatg aggtatccca gcggctcaat ggcccggcgc agtgcgctga tggtcccttt
     1561 gtgtcggtgt atcagccatg catcacgaat aacctgtcgc tttgtctctt ccggccagtt
     1621 gcgatcccag cggtcaacgg aaaacgccca ggcgagataa ggcagcagat gcaccgggca
     1681 ggtgtccggc gaccacagcg tgttgaggtc taccggaatg tctgtaatgc gtgttccgac
     1741 ggcttcggca caacgcatga aattgctggc tgatggtggt aacaacgaat tactcattgc
     1801 gtccaccttc gctgatggtg aatgacgcac agcgcgccgc ctgtatgtcg ctgatggcca
     1861 tattctgtgt gggttcgatt atctccacgc gttgcacacc gtgcacatgc agtgcggcag
     1921 caatggcgga caacgccacg tcctgaccga taagcccctg ctcagccagc cacttcctga
     1981 acgacgattc agccgcggcc agaataggtt cggattccgg gccggggtaa aagtacagtt
     2041 ttgcattcag ccgccatgtc acgattctgg cactctgtac ggtcaggcgg tcggccaccg
     2101 ggcgggtatc ctctgcattc agaacggcgc gaacggtatt aagcaacgcc tccgttgctg
     2161 tgccgtcgcc ctcagtggac aggatggaaa ccgtcacgtt ggccggagac gggctgatgg
     2221 cccgcgcatc gcgtaccaga ccgctggcgc tgcgtgcaaa atactcgtat gcacctgacg
     2281 ggccagcaac actcagaccg tcatatgccc gttgcgcccg tagtctcagc gaggtgtcac
     2341 tttccatcac tgcatcggtg gtatccgttg ccggagtgat aaccaggcgc tttgtgttca
     2401 tattgcccgc gaggttgtcc aggtctgtcc cggcgctgtg gcttagcatg caggcgcgtg
     2461 caccctcatt gacccgctgg cgtaacagca tttcacgaaa cgacatggtt tgagcgataa
     2521 cgttaagggg ttccgattcc agctccagcg cggcggaaac ggcttcacgc tgttcggcgg
     2581 gataggacgc aatcatcatg gcctttgtgt cagccagaat tgcctcaaag tcaggctccg
     2641 cgatgatggc gggttccggt aactgtgaaa ggtcaacggc aggcatgatt tactccctca
     2701 gcgtgatggt taattcaaca ttctgcatgg tctgcatgac agtgcccgac agcgtcaccc
     2761 cggcgcggcc tcctgccttc cagacaacgt cgatagcgtc cagggcaatg cggggttccc
     2821 atcgtgtcag cgcaatcacg gcagcactca tgcattgcag acgcgtggtg ttattcatgg
     2881 gttcgtcaat caaatcaggc acaaggctgc catattcccg tcgcataacc cggcttgcca
     2941 gcggggtggt caggatgtcc ctgactgact gtttcaggtg ctccatatcg ttcaggtttc
     3001 ccgttccgtc cgggttcatt cctgtgtagc gggttgtcac tgcgggcctc ctgtcgaatc
     3061 gctgccacct ttaacgccac cgtgtttatg cgtatgcact gtgatgccgt ttgaggtgaa
     3121 attaccgccg ctgtgcgtga tattgccgct catctttccc ccttttgtga cgtcaagcgt
     3181 cgccgttctc agaaggtctg tgcattccac gacgggcgta tccagtgtca cgctgacgga
     3241 tgcctgcagg gtggctgttt tcatgccgct ggcgctcagt gcgcctgcgt ccgcgtcgta
     3301 gcggaacacc gcgccatccg gcgcgctgac cacgatttct ttcaggcttt tgccgggggc
     3361 cggaatggca tcactccaca ggctgccaat tatcatggcg gtttccgggt tgccgccaat
     3421 gcaggcaatt accacctgtt cgcctggtga tggcggcagc cacacattga aggctcccgc
     3481 gcgagaggtg ttccagcgca gccaacctgt ttccagttcg ccgctgcgaa cgcgcacgcg
     3541 ccaggacttc tcatcaactt cagagatgat cccggtgcgg atgatattgc tcagtagccg
     3601 catgagttct gcgctcaccg tacagcctcc gcaatccggc ccagcaccgt gttataaatc
     3661 aggcgctcat ctgcctggct gatacccagc agctcacgta ccgggtaatc ggtgaaaatg
     3721 cccggcgcaa cctgatcgcg ctcaccgaac tgatgaacgc gtgcaatacg tgcggccacg
     3781 ccgctgtaac ccaccgtcac accggaagca tctgcacggg ctttcaggta gcgggcggtg
     3841 cgcagtttta cgaacatggg gacgcgcttt gtgctgtcct ggttgatgcg ccgggtgcgt
     3901 atttccagaa agcggtcgat gtcatcccgg taaaacgtgc ggatgctgtt tttatcctca
     3961 tcccacccgg tgatggttcg cccgtatttc cccgtgtcgt gatgccagtt tttcagcgtg
     4021 cgtgcttcgt tattccagat aaagcgaatg cgctcctgta tccgggttac gtggcgtctg
     4081 cgtggtgtcc atgcggtccc gtccggcgct ttctgtggcc ggatacgtgc ctgctgggcg
     4141 cggcgtaaat cctgtgccag ctttctggcg atgttattga tggcctgctg attcaggctg
     4201 tcgcggatgg cctcaaaggt ttcatccacg cgagtgaatg ccttatccat cgctttcacc
     4261 ccacgtcaca tcctggaata catgcgacca gtcgccttcg gaagatggca ggcggggttt
     4321 tggctccagc aggtgttctg cctgcggtgt gccctgactg ctgcgcgtga tgcgaacgcg
     4381 ttcccgcagg gggagcgtaa acaggagatc ggcgctgtca tcgtcattga taacggcgga
     4441 aaatttgatg tcctgattac gctcagggtt gagcaacaac tgtggctgat tttcggataa
     4501 ccacgccagc agcggcagcg tgaggtcgtc cagctccccg gcgtaatcca tgacaaacat
     4561 caccatctga tagcggtaaa caaacgatgg ggtttctccg gtcgtttcaa tgttgccgct
     4621 ctccacgaaa atggtgaatt tttccgggtt agcctgacac catcggcatg aacgggtcat
     4681 ggcttcacgc agggaatcag ttttcagcat ggttgttatc ctcgttgttc agtcgttgca
     4741 gcctgcgctg ttccagtaat tcaatggccc gtttatccgc gttacaggtt tccagtgcat
     4801 ccagaaggcg gtcgccccat ataccgagat ttccccatgt gggagtgtca gggaaggggg
     4861 gaggcgttac cggtatggtc agcgtctgcg gtataagccg gactgacggc gctggcagtg
     4921 gcgcgttctg cgtgcctgcg cagcctgtca gtaaaacgag cgtcaggcaa agcgtgggcg
     4981 cattcatctt ttgcaatatc gttgcgtagc tgttcacgtc ttacctctcc gtcctgattg
     5041 cgttgctgat tttccacgcg gagttgcgcc agcacctgct gcatatcctg taccccggcg
     5101 ctgatgatat tcagggtgtc ggcggtactt ttcagggtgc tggcctgcgc ttcgtttctg
     5161 gcgttctccc ggcccagcga ccacgacaga cgcatggatg ttccccatcc ggcagtcaga
     5221 aggaaagcga cgccaagcgt gggccagagc ttcatgccgg ataggctccg tgtggtaact
     5281 gaaaatgcgg tccgtctttc agggtcttcc agtcgccgcc ccattccacc ggaatattca
     5341 gttcccggct ggcctgtctg aatgctgctg cgattttttc gtacagcggc cattcccatg
     5401 acatctggct gccgatataa gccacaacat ccacggcatg ccccgtaagg tggcggctgt
     5461 tcatggtctg gctcttaccc gtggccacca gttgcttctg gcggtaacgg ctgcgcaacc
     5521 cttcggtgat accaaaatcc acttccgaga tttccagtgc ccgtcgggtc actttcacca
     5581 gatcaggatt tacaccctgc aaattctttt cgctccggct gctgaattta aatgtgttgc
     5641 tcattcgtcc ttctccttca ccctgcgatt aaaggccgca ataaccttgt cgcgtgcttt
     5701 ctctgcaccc ataaaaccga ttgatgcgcc gataaacgtc acggcatctt caggaaaccc
     5761 gaagaagcgc aacgacccgg ccacggccat ggcaagaacg ccgcacgcca gcgatcccgt
     5821 tacggtctga accagtgttc gtccgtcata aaggctcatc agcgcggaaa tgctgaccgc
     5881 cgcgcctact gcatacaccg ttggcaggtg gtcaaagagc cacgcaataa cctgctctgt
     5941 gatccctgtt tgaatggtgc tcactgctac tccccccaca actgaatcat ttctcgtttc
     6001 ttcttctccg gctccggcat ctccacttcc tgcccggcgt ccagaaatac ctgctgacag
     6061 agtccggggt tggcatccag caccttttcg gtgacgccct gcgtcgtgcc gtagtaccgg
     6121 aaacagagcg aatccacggt gtcgccttcc agtgccttca ctttcatcag cacaactccg
     6181 caaagattcg cgggcggcac agaatgtcag agatggccca gctcacatca cgccacaaat
     6241 ccgatgtctg tatatccaga gcgtctgccc ggcggtcgcc cttgtccgta gtgtctgcat
     6301 cacgataacg ctccagaatc agggcgcgcg tggcggtata aacagcattg cgccagtgcc
     6361 agagattgac gctttctccg ttaattacgg gtgccggaac atcggccagc gtctgatggc
     6421 cagctgcctg ctgttcctgc tgccatgctt ccagctcgcg ggtaacgtgt gccacagccc
     6481 cggtggcagt atgcagcagg cgggaggtgg tcacgcgccc cggcagtcgt accgccagac
     6541 gcagctcgcg cagcacaata tccggccaga atgcacccgc tgaaatgcgg gtatcgccat
     6601 catcggtatc ggtgatgtcg tcctctgcgg gtccgggttc agttctggca actatagtca
     6661 tggggttcac tcctgaaaaa aaatcgggcg gtgggtgcgc ggtgtaaacg gtcacggagt
     6721 caaactggaa caccgcgcac gccgcccgct gacggggtca gtcgttaacc gcgcttcgcc
     6781 ttctgcgtcg cggtggtttt tcgtgttgca ggcttccgcg ttgtcttttt acttttgctg
     6841 ctttttgctg ctttcgtcct gcgcctgctg tgcgctggcg tcttctggtt cggctgcgga
     6901 atcggctttt ttcagggcgc gggaaagggt tgcaatctcg cgtttcacac ctgcgttcgg
     6961 gttcaggtgc attgcttcgc gcagcagctt cagtgacagg gccatgctgt ccgcatcact
     7021 caggccacgg cgggcaaagg cgcacgcttt gcataatttg gcgcgcactt cgtccggcat
     7081 gtcctggtcg gtgacaatct cccggagggt gtccagtggt tcgataaagg cggacaaatc
     7141 cgcgtcggca tccgtcccgg cctgcgtcag taccggatta cagatttctt cggtcagtac
     7201 cgtggcagca gtacggccaa agttatccgg catgatgagg ttgtgacgga ctacatacgc
     7261 gccgatacgc agcgcaagcg gaagatcgcc acagtcaatc gcccacacca tcagcgtggc
     7321 aatcacttca tcctgctgcc cgccgtcagc ctccagcgtt ccctcaatcc agccggaaaa
     7381 atccggcaac aactcttttt tgatggcggc tttcgcgctt ctggcctgta cgcccttaag
     7441 ccgggcctgt gccagacgca gacgatacag cacctcttca tgcgcggtac gcgcggcgtg
     7501 gtccacgcct tcattcgccc ggcctgcgcg ctgtgccatc acgttctgcc agtgttgttg
     7561 tgcaggagta atcatttttg ctctccgtta caggcgggca tgatgcccgc cgtgagttga
     7621 ttagctgtcg gcgaacttca ggccagtaac catcgcgcac ttgccatagt cttcaacgac
     7681 ataagcgtca ttgatggact ggtaggtggc gatgcggttg tattccggct cgtctttcat
     7741 caggcgacgc attgaacctt tctgccagta aatcgacagg ttgttgaacg aggtgatcag
     7801 catcgttgca tccgggaaga acggcgcaag gaacacgccc agcccgccaa tggtgcgcga
     7861 tgacaggatg agctgcccgg cgagtaattc cgcattggga ttctggccgc tgatgctgtt
     7921 cagcacgggc agacgcagcg agttaaacag gttgcgcccc ataatcacca cgaggtcgtc
     7981 agcttccttg tgccattcat ctagcaggga tgaacgcgcg tcctgtacca gtgcatcagc
     8041 gttcgcatac ttacccgcgt gcgccacggt gttgtccatg ttgcgggagg tcagcgtcac
     8101 gtcattcata acgcgttcgc tggcgtcggt tctgatgtgc tccagccagc ccacgttaac
     8161 gtcctgaagc agcttgttgg tgctgaagtt ggactcatcc gcgtgagacg tgccgttgaa
     8221 accgatcatg atgcggtcaa gcgccacctg tcgggcaatc tgtgcgctga tgcgggactg
     8281 aaaatcaggg tgtgccgacc aggcatcaag ctgcggatac gaaataaacg tgtcgtagtt
     8341 cacctgttca cactgatatt tgcggttttt cagatcaacc acgttattcg ggttacggcg
     8401 ttttgtgccg tcataactgg tattcgtgcg cgcaatcggt cctgtggtat ccaggaggat
     8461 tttttcgcct ttctggtcgg tcacgccgat cacgttattt ttttcgtaaa ttcggtactt
     8521 tcctttgagg cgttttcaaa acgctgctgc accgcgggtt ccacggtaaa tcgcgacacc
     8581 agtgcagata ccgggatatt attaagcgac acctgctgcg ccatatagca acccagcttg
     8641 ttgcgggtaa tatctgacat caccagattc ataaaaaatt tgctcctttg tctaatcaga
     8701 agtcggccag ctggtcggag gctgcgcccg ttgcggtgaa gcggttctgc ggatcgccgt
     8761 cctgcgtgcg cagtttttcc ttcagtgctg ccagctctgt ggtcagtgac gtaattttct
     8821 ggcggtcctg ctgatggcgg gtttccagca cattaaaacg gtcgataatg tcggcctgtg
     8881 acgttgcgac gccttccacc gcttcctgaa tacgggaaaa actggcgtca tccgctttgc
     8941 ggccacggcc aataatcccc attacgcggt taaaccactg ggtgccttct tcctggcgtt
     9001 gttctgccat ttcgatgatt tcagactcga tggcttcgga gataagcggc gcttcaccct
     9061 ggatactgtt aaacgtcatc accgcctaac gttgctgtgc cgtgaatttc aggcgctcag
     9121 tgcccaggct tgccggggtg tcggtcatcg ccagcccgac cagataggcg cgcccgttaa
     9181 cggagaactg cgggtgcagt tcgatactgg aatagatttt cttgccgtcc gcaacaagct
     9241 gcttcatgcg ctcggtcggt tcgatttctg catacagcgc agtacgtccg gccagcggac
     9301 cttccgtaat gtcttccgta ctcagtgcgg tgacatcgcc cattgcggaa aattcgcttg
     9361 acgggcatgg cgagagatag tgctcaacgt tcacgcgggc agcgtaaaca tccgggttga
     9421 agttctcggc ggcttcacgc agatgcaccg gactgatttc acggccatca acagttgatc
     9481 cggagacagc cacgcgaaac tttttgcggg atgtcttttt ttcattagcc atagtttttg
     9541 cccctctgac tggttcttca gtcatgatgg caaagcgtaa caggctgata caaagggctt
     9601 ttgttgtaag aaaacggcca gaacagaggg ttaaggagaa cagtttcgcg cgcgggtaat
     9661 cttcctgtaa ttactcaggg ggagcaatga ttcaggacgc ttttgtgcgc cagcgtgcgc
     9721 ggcaacttta ctggcagggt tatccgcccg cagaaatatc acgtctgatg ggaataaacc
     9781 cgaacacgat ttatgcgtgg aaaaaacgtg accagtggga tgaaacgcca cccgggcagc
     9841 gtgtcacgca gtccatcgat gcgcgcctca tccagcttac tgaaaaacag aataaaacag
     9901 gcggtgactt taaggaaata gacctgctga cccggcagct taaaaagctg catgatggcc
     9961 agccggatgt gatggccgca ggaaagaaag gccgggcgaa aaaactcaaa aatcatttca
    10021 cgccggaaca gattgccgca ctgcgggaaa aaatcatcag caggctggag tggcatcagc
    10081 ggggctggtt tgactccctg accctttgca gggaagccgg gatacgtaac aggatgatcc
    10141 tgaaatcccg acagattggg gcgacctggt attttgcaca ggaagcactg ctgatggcgc
    10201 tgcgtgacga tgtggcgcaa ccttaccagc gtaaccagat ttttttgtct gcgtcgcgtc
    10261 gtcaggcgtt ccagtttaaa agcattattc agaaggccgc ggctgaagtt gatgtggagc
    10321 tgaaaggggg cgataaaatc atcctctcca acggcgcaga gctgcatttt ctcggcactt
    10381 ctgctgcgtc ggcacagtcc tatacgggca atttttattt tgatgaattt ttctgggtca
    10441 gtcgctttgc tgaactgcgc aaggtggctg gcgctatggc aaccctcagc ggactgcggc
    10501 gcacctactt ctccacgcca tccaccgaaa cgcacgaggc atacgcctac tggaacggcg
    10561 accgctggaa cgagaaaaag gcctcgcata aacgccagcg tttttctgtg gactggaaaa
    10621 cgctgcataa cgggcttatc tgccctgacc ggacgtggcg gcaaattgtc acgctggaag
    10681 atgtggttaa tcacggctgg aaacacaccg atatcgacga aattcgtgat gaaaacaccg
    10741 aagacgagtt cctcaatctc tatatgtgtg agtttgtccg cgaaggggaa tcggcattta
    10801 acctgaatat cctgattggc tgcggcgttg acggatacga cgactggaaa gactggaaac
    10861 cttttgctcc ccgcccgatg gggaatcgtc cggtatggat tgggtatgac gcaaacggca
    10921 gcagtggcaa cggcgacagc ggcgctgtgt ccgtggtggt tcctccgtct gttcctggtg
    10981 gccgttttcg aacggtggag acgcgacgcg ttcaggggct ggagtttgaa gaacaggcca
    11041 gagtcattga agagttcacg tgtcgctaca acgtggaaca catcggcatt gatgtgacgg
    11101 gcgggaacgg ggaggctgtt tatcagatag tgaaacggtt tttccctgcc gctattccgt
    11161 acaccttcac gctgtcatca aaacggtcgc tggtactgaa aatgctgcaa ataatgcgtg
    11221 ccgggcggtg ggaatacgat cgcgccgaac gcgagctggt cgcggccttt aacgccgtgc
    11281 gtaaggtgaa aacaccgggc ggctttatca cttacgaaac ggaccgagcg agggggatca
    11341 gccacggcga ccttgcgtgg gcaaccatgc ttgctgtcat taacgaaccg attggcggcg
    11401 aaggagaaaa cgagcgtttc acggttatgg agttctgatg agcagaaaaa ataaaaaagt
    11461 gcgcatgagt tcacgcattg atctcgctga tgcgctcagg aaagaatcat cgctcagtgc
    11521 attcacattt gatggtcctt atcgcctgac ctgctggaca atatgtactg tgctgataac
    11581 gggcggtggt atgaaacccc ggtggactgg tacggtctgg caagagctgc ccggcaaacg
    11641 tcctggcatc agtctgcgct ttattttaag cgcaatgtat tgctcggttg ctacatcccg
    11701 cacccgctgc tttcccggca ggatttctcg gcgctggcgc tggactggtt tgtgttcggt
    11761 aacgcattcc ttgagcttcg aagcaatatg ctcggcgaac cgcttaaatt acggcacgcc
    11821 ctggcgaaat acatgcgacg cggaagcgat cttgaatcat ggaggtatgt gcaggatggc
    11881 aaggatgcgt ttcagtttcg cccaggcaaa gtgtgtcacc tgatgaatcc tgacattaac
    11941 caggaaatct acggcatgcc ggaatatctc ggcgcattac tctcggccag cctgtctcat
    12001 tcggcggaca tgttcagaaa actgtattac gacaacggat cccacgccgg gtgcatcatc
    12061 tacatcggtg ccgcgcaggt aaaccgcgaa agcatggact ccctgaaaga aacgctacag
    12121 ggtgcgcgtg gtggtggtgc gtttaaaaac gtgctcattc atgcgcccaa cgggggcaaa
    12181 gagggggtgc aaattttgcc gttccagcag atcaccgcaa aggatgagtt catgaatgtt
    12241 aaggcggcat cccgtgatga tgtgctggct gcgcaccgcg ttccgccgca actgatgggg
    12301 gcgatgccgg gtgaaaaaag tgcgtttggt gatgtggaga aggccgcgcg ggtttacgca
    12361 attaacgagc tgatgcccgt catggaggcc atgaagcaca tcaatgactg gcttggcgaa
    12421 gaggtgatcc gctttaaccc ttacgcactg ttagacatcc agcccacatc ctgacgcgct
    12481 tcgcttgtct gctgcttcgc cggggcataa aaaatttatg ccccgactct ccagctcctg
    12541 tatcagtcag ataatttcac gacgccttcc agtttatcgc caccatcgac ggtcagactc
    12601 ttacgcaatc ccaccgcgtt gactgcatgt tctcgccgcc tcagtgcgat tttgacggcc
    12661 ttacctttca ccccatcaaa tcagaatccc tcacgtattt ttcacgctca gcgtgagaaa
    12721 tacggccatt ctgtcgtgtc gctgcgacat cgttaaggga acgctattta ccccctgaaa
    12781 cgcgggctgt tcccccgtca cctgcgcgca gaaaaagcgc gtttttttgt gcacgcacgg
    12841 atccttgacg gatccagccg ccacgcgggc tggaagggca aaaattcgtt caaaaaaatt
    12901 gtgcaaatgt gcagcagggc gatgtgcgat tatcgccctg gtttgagggg gatcaggtgt
    12961 tatttttatc tgtcgtcagt gggctaaacc atccacaatt tacatcgcat gactgatgcg
    13021 ttctgtcctc gtgtttctcc tgccacaatg cttttccgcc agcctttatg atggctcttt
    13081 caacatcccg ctcgtatcgc aagggctgaa atacgtcatt aatgaaatat tcgcttatgt
    13141 ccggaagcga tactgcaaaa ttatttgttt gttgcgccgt cctgctatgc agcaaagcgg
    13201 catgctttaa tgcgtcctgt gctttctgga atttaagtgc atagtcagtg gcgatttttt
    13261 ccagttcagc gattcggttg tctttggttt ccagccaatc aagtagcgcc aatacttcag
    13321 gatccccgac atccaacact gttacgcgtg atttttcata atgtttgtca gcaagagttc
    13381 ggccaatttt gaaatctcca tcatcaccat aaccggagca ggcataaaca acctgtgctc
    13441 cagatattcg ctgaattgac atttcctcgc cgcaaatagg gcatttcggt actggcacag
    13501 gtgaataacg ttcacgtaat gcctgatagt tgatttcgct cattaagtcg ttctccttgt
    13561 ttatgttctg aggtggagag cagggcgaac tcgccctgat gttgtgttat tcaggaaata
    13621 acgcccggat atttccggcc atctgactgg ttatctgtgc ggttgatact ggctgtgatg
    13681 cggggcgttc tgtcctggtt tgtgtcactg ataacgcttc atcaccagcc catgcagcca
    13741 gtcgataagc ctctgccgga ttcattttca gaagtgccag cccggccaga aaagccacgc
    13801 gttggccgct tttgcgggct tctggtgtaa ggctgtccag ccaggcgcat gcttctcctt
    13861 cgttcttgac ggcggtgggc ttcagataga aacttatccg tctggttgga gtcgtcattg
    13921 gtttactcct tgtccattgc gtacagccca ttaaccagag caaactgtgg caccccgtcc
    13981 gcgatgaaag tcgcattaac tccgcaggct tcgcggatag cgggtgccac aatctccgcc
    14041 cctccaccga caaccatcac ccgcccgtaa cccgaaaaaa ccgccagcgc gcggatcacg
    14101 cgttgtttca gtgtttcttc cttttcacga attaccgcca tcaggctggc gtaatgcgcg
    14161 tcattgtgga tgtgctggcg cagccaggct tcatcatggc gatgttcgat aatggtattg
    14221 gcgatgtggt gactggtgcg cataccgtta gtggccatca ccgacagtac ggcatcggcc
    14281 atcagagaaa cgcccacgtg tggatcgcaa aacacctggc tgatacctgc cagttgcccc
    14341 tgaacctttg ccacatccag cgtggttccg cccaaatcca caatcagcag ggattcaaac
    14401 ggactcatgt cagccagtgc cttaaagcca gccggaatgg attcaggcat aacctgtacg
    14461 ttacggatag tgaatgcttc gccgttctgg tactccaccg ggcgcatgac gttcgctttt
    14521 ttgcggtt
//
LOCUS       DAJTNN010000099        14342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTNN010000099 DAJTNN010000000
VERSION     DAJTNN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.099"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..791)
                     /gene="fimH"
                     /locus_tag="OO150_002641"
     CDS             complement(<1..791)
                     /gene="fimH"
                     /locus_tag="OO150_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5516735.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAV"
     gene            complement(811..1314)
                     /gene="fimG"
                     /locus_tag="OO150_002642"
     CDS             complement(811..1314)
                     /gene="fimG"
                     /locus_tag="OO150_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5516736.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGETKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(1327..1857)
                     /gene="fimF"
                     /locus_tag="OO150_002643"
     CDS             complement(1327..1857)
                     /gene="fimF"
                     /locus_tag="OO150_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5516737.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(1867..4503)
                     /gene="fimD"
                     /locus_tag="OO150_002644"
                     /pseudo
     CDS             complement(1867..4503)
                     /gene="fimD"
                     /locus_tag="OO150_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
     gene            complement(4570..5295)
                     /gene="fimC"
                     /locus_tag="OO150_002645"
     CDS             complement(4570..5295)
                     /gene="fimC"
                     /locus_tag="OO150_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5516738.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQFAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(5332..5871)
                     /locus_tag="OO150_002646"
     CDS             complement(5332..5871)
                     /locus_tag="OO150_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5516739.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(5936..6484)
                     /gene="fimA"
                     /locus_tag="OO150_002647"
     CDS             complement(5936..6484)
                     /gene="fimA"
                     /locus_tag="OO150_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5516740.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQIVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
     gene            complement(6965..7561)
                     /gene="fimE"
                     /locus_tag="OO150_002648"
     CDS             complement(6965..7561)
                     /gene="fimE"
                     /locus_tag="OO150_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5516741.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(8039..8641)
                     /gene="fimB"
                     /locus_tag="OO150_002649"
     CDS             complement(8039..8641)
                     /gene="fimB"
                     /locus_tag="OO150_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCR5516742.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            10097..10813
                     /gene="nanC"
                     /locus_tag="OO150_002650"
     CDS             10097..10813
                     /gene="nanC"
                     /locus_tag="OO150_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5516743.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSSDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            10833..11939
                     /gene="nanM"
                     /locus_tag="OO150_002651"
     CDS             10833..11939
                     /gene="nanM"
                     /locus_tag="OO150_002651"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5516744.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNREGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQHGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            12004..12984
                     /locus_tag="OO150_002652"
     CDS             12004..12984
                     /locus_tag="OO150_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5516745.1"
                     /translation="MDAIISPDYYYVLTIAGQSNAMAYGEGLPLPAREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLSFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLIMGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWREK"
     gene            complement(12992..13114)
                     /locus_tag="OO150_002653"
     CDS             complement(12992..13114)
                     /locus_tag="OO150_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5516746.1"
                     /translation="MISFVFLLLVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            13196..14068
                     /locus_tag="OO150_002654"
     CDS             13196..14068
                     /locus_tag="OO150_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5516747.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGSVERLFWDFDGKIIALPQVRKNYPY
                     EGFVEWHRNEVFRG"
     gene            14113..14292
                     /locus_tag="OO150_002655"
     CDS             14113..14292
                     /locus_tag="OO150_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516748.1"
                     /translation="MQDLAASLQLAFDSGAKRVLLPISWAMDIPTVPAELFTKFQVSF
                     YSEPVDAVYKALDVN"
BASE COUNT         4010 a         3315 c         3143 g         3874 t
ORIGIN      
        1 actgctccta acgataccgt gttattcgct ggaataatcg taccgttgcg cgtcaactgt
       61 acgccgacgc cctgcgctgg tgaaaacgac gcggtattgg tgaaaatcga gttgcccgca
      121 tctgcggttg tgccggagag gtaatacccc aggttttggc ttttcgcaca ataaacggta
      181 agaggaattg gcactgaacc agggtagtcc ggcagagtaa cggtgacatc acgagcagaa
      241 acatcgcagc cgccagtagg caccaccaca tcattattgg cgtaaatatt ccacacaaac
      301 tggaaatcat cgctgttata gttgttggtc tgtcgcaaaa taagcacggc aattaatgag
      361 ccagctttaa tcgccacccc gcccgcactg ctcacaggcg tcaaataaag cgccaccggc
      421 cacggcttat ccgttctcga attataaaca acccgcggcg tttcgctggt ggtcggaaat
      481 ggatagctac tgccactata ttttacggtc ccggaaaaat tagataacac gccgccataa
      541 gccgagcctc gttgcagtgt gacatagtct gtaatggttt ccggataatc gttatggcaa
      601 aagatttgcg tcgaaagatc tacgaccagg ttttgcccca cattcacgac aggcgcaagg
      661 tttacataaa cattagcgct gccaccgcca atagggatag cagtaccatt ggcggtttta
      721 caggcgaatg accaggcatt taccgaccag cccatcagca gtacagcaaa cagggtaata
      781 actcgtttca ttacaattat ctcttcgggt tcagctgtag gtataggtga tgctaatcac
      841 tgcctgaatg gttccctgag tggctccgcc atttaccgtc aatgctctga cctgtaacgg
      901 gaagtgcgct gattgtgagg aatcatccac ctgaactgtt ttggtttcgc cagtattcaa
      961 tgtgttgcca ctgtcatcct gtagctctaa ctggatgttt tgcgcggtcc cctggttttt
     1021 ataatatccg gtactgtcgg ctgccccgct gaagctggca gtgaccctcg acgttcccac
     1081 cggacaatta gtcaactcaa gcgcaacatc atgccaggcc gatgccgccc cggcagacat
     1141 cagactgaaa gaataaagat cgccgagatc aaccgtggca ttggtggtgg aaactgtgca
     1201 cggtttggcg acgaccttac cgttcaccgt gatggtgaca tcggctgcct gtattgtcgc
     1261 acttgcgagc gccaatattg ccgccaataa atacccacgt ttgcgccatt tcatgagcat
     1321 ctccagttac tgatattcaa gagtgaaggt agccgtggca ttgatatgcc ccgcagtgac
     1381 aggcacctgt gtcgccatta gccgggcgta aaaattcaac gtatttggtt tacccggcgt
     1441 cagggtcgtc cacgaaatcg cggacgatgg agcattaagg ggtatctgat tttgctgctc
     1501 attcagaagc tgtattccca gtcccgaagc cgctgacacc gtattttcaa gtgcaagcag
     1561 gttggcattg tggctatctg caacgccggt aaacccaacc tttacggcag aaacggcgtt
     1621 accacagggt gacagcaaaa tacgaaatgg aacgacagga gtcgttgcgc caatgttgtt
     1681 aaattgcttc gccgcgtttt ccatcagatc aacagtaaaa ttggttgatt cagcggccac
     1741 actacagccg ttatctctga catagccgcg gatagtaatc gtgctatccg cagccaaaac
     1801 gcgacttacc gccagccaca aaaaagcgca cagaagataa aaaggtttgt ttctcatcac
     1861 gcccccttaa cgacattcag ctgatagctg ggttaataac tgctgctgac tctctggtgg
     1921 cagttgataa ttggcgatac agtgagcatt ttcctcttct ccccatttca cctgaacttt
     1981 tcccgctaaa ggcattccgc tgaggtaaac ctgaccatta tccgcaacaa tgccgctact
     2041 ctggctactc tctgatgtca ccatcgcccc aaacggcagc ggcttattat tgtgggttag
     2101 cgtcatgagc agttttatcc caacgcgcgc tttaaactct gctcgcacga tcgccccacg
     2161 agtgggaaca acgttagcga ccgcgttatc taaatcgacg ttatcagcca gggtattggt
     2221 atccagcgcc actctgtttt cccgatattc ggtggcataa ggcagcacgg cataaccgcg
     2281 ccagtcggta cgcacccccg tctggttttc gacttttgca tcttttgcgc caggcgcttt
     2341 aacaagtacc accgtatcgt ttaacggctg ccccagcgtt acgccattgg catgagccag
     2401 taccccaccg ctgactccgt aatagagctg cttaatatca tcgctatggc tgtaaccgat
     2461 attggcattg ccgtaaccac cgcgataatt cagcgtggcg tagcctgtac ttccgctatt
     2521 accatcgcct cccccggcat agccggtttg cacgctatag ctgaggttgt tgtcttccag
     2581 caacgtaccg tatacaccag ccagattggt catccgaccg ttgagatcgt gtgacacgct
     2641 gtagctggca ctggcatgtc gccactgaga tttactgtca gaacgcagcc agtggctgaa
     2701 aggaatattg acgttaagcg ctaacatctg atcccgccct ttttgccagg cgtttttcgt
     2761 caggctatag ctgagcgtcc agttgatatc ttcgaacgca gtatttaatc cagcctagaa
     2821 ttgctcatcg acattactcg ttccccaata agtttgatgg ctaccactca aatacagtgt
     2881 tgatgtgcgc ccgagttgct gagtaacggt gagctgtaat ttcccgcgtt tgttataagc
     2941 gaggttgtaa tagtcggtga atttcggctt aacctgaata actccatcct gtgtttcgat
     3001 gttgtagcca ttcattcgac tgtatgttgt atcagcgaaa ttaaaatatc cgctggtcga
     3061 ataacggtaa cccactaact gaatattcgt gcctgactca ttgagcgatt tgttatagag
     3121 aaaacgcacc gattgtccgt catgctgact gtcatcggga agtgtggaat tagcctgcgt
     3181 catatccaca gacagagcgc ccagtgcccc catgtttttc ccgataccga aattaaaagc
     3241 acgataacga tccgccagtt gcgttccacc atatattgtc cagccagccg gaaggccgtg
     3301 gagtaatgta ctttggaaaa agcggggttt ttcctgctgc gtatttccac tacggtattc
     3361 tcctgccgta atggaataac gagtatgccc ttcacgttgc aaaagcggga ctgacgaata
     3421 gggtacggta aaaatctgcg tgctgccgtc agcctctttg atcgttacct gcaagtcacc
     3481 actattacct gcggcataga tatcgttgat ggtaaaaggc cccggtggca ccgtactatt
     3541 ataaatgtca tacccatttt gtttaatagt gacctgtgca gtaccacgag caataccgtg
     3601 gatcaccggg gcaaatcctc tttggctatc gggtaacata ttgtcatctg aggccaattg
     3661 tgcgccgcga aagttaatac catcgaaaat atcgccctgg gtataaccat cacccagcgt
     3721 cagccgggaa cgtaacggta ttatgtctcg ctcaagccag gtattgatat gctgccattt
     3781 atttttgcta cctgatgatc tgtcgctact gttataactc cagctggtat tgtcgcgtaa
     3841 acaccacgca ccaatattta acccactctg taggtttaaa tatgcataat ggctgttacc
     3901 cccaatctga ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat
     3961 accgggatcc cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg
     4021 agggatcgtc aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcatggat
     4081 cattgaggtt aatggcacgc aggcatcatc tgccagcaga ttcataccgg agacagaagc
     4141 cgcattcagc cccatactgg caagttgcgc gcgtgtcagg cagggaacaa tcccttgttc
     4201 actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat
     4261 atcgacgcga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc
     4321 cacagcctgg ggatcatccg ctaaaaagcg cggattaaaa tagagttcgg cagatgacaa
     4381 aggtgcctgt gcggcaaaag cacaggcgac aacgagccgg acaaaaaaac cagccaaacg
     4441 atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga
     4501 cattattcct ccggtttaaa attacccggc agtcaattct ttttattcag gcgagaaatt
     4561 ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat
     4621 tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat
     4681 tggaggcacc aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag
     4741 ataatagggt gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt
     4801 ttctgcggcc tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg
     4861 gctgataatt gcgaactgta gcgtattctc agtcaatttt gatttatcca ttgacggaat
     4921 cgctttaaca ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc
     4981 atcaagaata cgcaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac
     5041 gataaaacga ccatccttta caccatcggc attttccatc catgattgaa ttaaataggt
     5101 actattttca tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat
     5161 tacgcgagtc gcacctaagg ccactcccgc ttcagcgcgt ccggcaacca tcattgccat
     5221 gaacatcagg atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattggc
     5281 gtttttatta ctcactgtcc tgttcctgtt atcacattat ctgctgaaca attactgata
     5341 ggttaaagag aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc
     5401 acgatatttg gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttac
     5461 tggaggacga ttaatcggta cgaggtttcc ttcatcatca aacaacgcta cgccaatatt
     5521 ggtggctatc cctggcccct ctcccacgga aagcacatcc ggatttttac catccgcgac
     5581 accgtgaaac gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat
     5641 aacaaaaggc accggtgcgc tatcttcccc taccgcatga aaccggttac tgctgatttg
     5701 ccccatattg accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat
     5761 gacgccctga aattgcatat ttccgccggg cagcgtagta ttccatttat ttccggccag
     5821 agcaaacatt ggcagcaacg acgctaatag aaataatctc ttccttttca tcgttatttt
     5881 tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc ctgaacctgg gtaggttatt
     5941 gatactgaac cttgaaggtc gcatccgcat tagctgcgcc aggggttgcg acaccggttg
     6001 cataataacg cgcctggaac ggaatggtgt tagtaccgtt attcagggtt gtttgctcac
     6061 tgaatgtcgc accatccagc gtcagcgcag cacccgttct gtccaggatc tgtacaccaa
     6121 cgtttgttgc gctacccgca gctgaactct gcagagccag aacgttggta tgacccgcat
     6181 caatcgccgt acctaaaaag gcaactgcgg ctttagatgc aacattggta tcgcaatcat
     6241 tcagctgaat gttaaaaccg acagcagaac tggttgctcc ttcctgtgcc agcgatgcgg
     6301 tacgaacctg tcctaactga acgatttgat caacagagcc tgcatcaact gcgcaagcgg
     6361 cgttaacaac ttccccttta aagtgaacgg tcccaccatt aaccgtcgtg gcagcggcca
     6421 gagccgctgt agaactgagg gacagagccg acagaacaac aattgccaga gttttaattt
     6481 tcatgctgct ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca
     6541 ctttgttttg tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa
     6601 catttggggc cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt
     6661 tgttggctta atattctatt gttatcttta tttatagatt tttatattgc atgaggtggt
     6721 ttttggagag aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag
     6781 cggaggtgat gtgaaattaa tttacaagag aaataattta catatcaaac agttagatga
     6841 tttttgtcgt tttttaaaat ttttatgctt gagaaaaaat acgtaactta tttatgatat
     6901 ggacaatttg gccccaattg tcttgtattt atttattttt taactttatt atcaataagt
     6961 taattcaaac ctcttctctt tttaattttt cgtttatgag attatttctt tcccataatc
     7021 cggcaaaacg agcagcatta ctggcggtat aacgtacagt atggcgaata tttcgatgcc
     7081 cgagataatc ctgaattaaa cgagtatctg caccacgctc cgccagttca taaccgcaag
     7141 catgccttaa catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcac
     7201 gaataatgcg ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatgg
     7261 cgtcggtccg gtcagcgcct ttccagttag cacgttccag ggtccagcgt tccacagctt
     7321 cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat
     7381 ttattcgacc ttcattaagg tcaagatcct gataatgcag atcaagcagt tcactaatac
     7441 gcatcccatg ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt
     7501 aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca
     7561 caatagtagt actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg
     7621 gtcaaaatta gcccaataga taaaaagata accacacacg aagtgatgtg gctatcagtc
     7681 aattacatta aatatcatac aaaaataaaa tatcactgat gtgacaacat ctattttcat
     7741 tgatttgatt caatataaca atagattgta gtagattagc ttccagttgc ttaaaaaata
     7801 aagaaaaaag tgcgaaaaca ggagaattta agtgatgtgt aaatttatga atgattttgt
     7861 ttatttattt tgaagaacat aaaccattgt attttgtatg gatatggtta acttaatgct
     7921 tattatgata attaagaatt atacttacaa taacatcaat taataaatgg ttaatcattt
     7981 ttgatatatc gtaagaataa tgtagttttt aacaccatcc ctggtatctc aactatctct
     8041 ataaaacagc gtgacgctgt cgtcctctgg ctctatccca gatgccgtaa aaacgccctg
     8101 cattgctggc ggtataccag acagtatgac gaatattgcg atgcccaaga taatcctgga
     8161 taagtcgcgt atctattccc atattcgcca aagcaaaacc acacgaatgg cgtaacatgt
     8221 gcggatgaat ctccagtgac aacccggcat taccaccgga agtcgagata atatggtaaa
     8281 actgttgccg agaaagcgga ttccccttac gcgataaaaa tacccactcg ctctcagcat
     8341 gcgggtacga agtacggata ctcaaccagt tttttaaagc ctgaacttct ttattcaata
     8401 gcgggggcgt tgttgaaaag ccttttttta atcgatggat atatatacac tttgccttaa
     8461 gatcaatatc cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca tgaataaaac
     8521 aaagcaaagt cagacaataa ttacgtgctg catgaggccc ggtatttgct gctttaagga
     8581 gtgattcgat ttcactatgg gtcaggaagt tccttttttt gttatcagcc ttattcttca
     8641 tcgtttttcc cttataatta cagacgcgca ctagctgtgc tgggttagca ataatccaac
     8701 atttttatcc tgattatgtt tataaaagcg aacgtttgct taataactaa cgtaagtgga
     8761 ccagttcttc tgagtgaact taaatggggt agcaacagtt aattataata acgttgccat
     8821 acaaacgcta tgctcttgca tgctatgtac ctttatatat ttatcaatcg gatacgaatc
     8881 taataatacc cctcatcatt aatgcaacta atcttatcta tatatcatgt gatatgttct
     8941 gtaacaagta aaatcagcat aaaaaattca gcaaaattta atattcgttc aactagccgt
     9001 ctcacatatg acattattag tcaactccat ttgccacaaa atggctattt catggaacgc
     9061 agtaatttct tagtggctat tatcatgcta attcttaggt ccctagcgat tattcctggc
     9121 tgattcgtca gcttacgcca tgagattgtt acattccttt ttatcctgga gatatggggg
     9181 aacgcaggag atgatttttt catctacatt cgttggtaaa tatagctttg gtcactacga
     9241 cttaaacctt cctcaatcac agccattcgc cgggcgatac ggcaaacaaa ctaagtgaaa
     9301 catcatcctg aaaacaccaa catcaacaag cctctccaga tcgacttcag aagtgaccag
     9361 ttacaagcca caaaccagga aacatatttc atgatgagaa ttatgctcag cctgatggcg
     9421 ggtaaacact gattttcacc aagctcactg agttagcttg caaagcttcc cttgtttaga
     9481 ctccaaataa attatttata aaacaaacat ttaaacaaag atctttagtt tttgaagacc
     9541 accgcaagtg ttcgtctggc ttcacatggc atcttcctct tagaaaaaga tcgacatatt
     9601 ttgtgacacg aattgcaaat ctggttttgt tgtatggatt gcgtgatttt tgatctggta
     9661 taacaggtat aaaggtgcac caagatagtc aatgagacag ggcatctcgc aatctatggc
     9721 aaacatcact tcagttcttt ctcatcgggt gatgaaaacg cacttcagtc tgaaaggaat
     9781 atgaaaatga gatcaacaga cactctattt tatgactctg ggtaaaatgg attgagtaag
     9841 tgatatagct tacgaaaatt caaatcaatt aaacatcaga agagatttta tactcaggta
     9901 tttaatctgg atctctgttt atttaaataa tgtgaaaaga gatttttcac aggagacctt
     9961 atacaaaaaa atataaaata cagctaccgg ttgccaaaga cactataagc ctggcaaaaa
    10021 atattacaca acataaatgc taattgttta tgcgggcttt gtattgcttt ctgtatccta
    10081 caaatgagtg aaatttatga aaaaggctaa aatactttct ggcgtattat tactgtgctt
    10141 ttcgtcccca ttaatttctc aggctgcgac actggacgta cgtggtggat atcgtagtgg
    10201 aagccacgcc tatgagactc gactcaaagt cagtgaggga tggcaaaatg gatggtgggc
    10261 aagcatggaa agtaatacct ggaataccat tcatgataat aaaaaggaaa atgccgcact
    10321 caatgatgtt caggttgaag ttaattacgc gattaaactt gatgatcaat ggacggtgcg
    10381 cccgggaatg ttaacgcatt ttagcagcaa cggcacacgc tacggaccct acgtaaaact
    10441 gtcctgggac gcgacaaaag atcttaattt tggcattcgc tatcgttacg actggaaagc
    10501 ttaccgacaa caagacttat ccagtgatat gtctcgtgat aacgttcatc gttgggatgg
    10561 atatgtcact taccatatta atagtgattt caccttcgca tggcaaacga cgctatacag
    10621 caaacagaac gattatcgct atgcaaacca taagaaatgg gcgacggaaa atgcatttgt
    10681 tctacaatac catatgacgc ccgatattac gccatacata gaatatgact accttgaccg
    10741 tcagggtgtt tacaacggca gagataattt atcggaaaac agttatcgca ttggtgtgtc
    10801 atttaaactg tagtagacag gagacagtca caatgaataa aacaataacg gcgcttgcta
    10861 tcatgatggc ttcatttgcc gcaaacgcgt ctgtattacc ggaaactcct gtgccattta
    10921 aaagtggtac cggagcaatt gataacgaca ctgtctacat tggtttaggt agcgcaggta
    10981 cggcatggta caagctggat acacaggcca aagataaaaa atggacagcg ttagctgcat
    11041 tccctggtgg accaagagat caagcaacct ctgcatttat tgatggcaat ctgtatgtgt
    11101 ttggcggcat tggcaaaaac agagagggct tgactcaggt atttaatgac gtacacaaat
    11161 acaaccccaa aaccaatagc tgggttaaat tgatgtcgca cgcaccgatg ggcatggcgg
    11221 gtcatgtgac ttttgtacac aacggcaagg cttatgttac tggcggtgtt aaccagaata
    11281 tcttcaatgg ctattttgaa gatctcaacg aggctggaaa agattcaacc gctgtagata
    11341 aaatcaatgc ctactatttt gacaaaaaag cagaagatta tttcttcaat aagtttctgt
    11401 tgtcttttga tccctcaaca cagcaatgga gttacgctgg cgaatcgccc tggtacggaa
    11461 cggctggtgc ggcggttgtg aataaaggtg ataaaacctg gcttattaat ggcgaagcca
    11521 aacccggatt gcgaacggat gccgtatttg aacttgattt caccggtaat aatttaaaat
    11581 ggaataagct tgctcccgtc tcatcaccag atggcgtcgc tggcggtttt gcggggataa
    11641 gcaatgattc tcttatattt gccggagggg ccggattcaa aggttcacga gaaaattacc
    11701 agcacggaaa gaactatgcg catgaaggcc tgaaaaaatc atatagcact gatattcatc
    11761 tttggcataa cgggaaatgg gataaatcgg gtgaattatc gcaaggtcgg gcctacggag
    11821 tatcattgcc ctggaataat agtctattga ttattggcgg tgaaactgca ggcggcaaag
    11881 cggtgacgga ttcagttttg atctctgtga aggataataa agtcacagta caaaactaac
    11941 gcttcagggc cccagtaaag gggccctgct atcaatttat catttcaatt acgcgaaaat
    12001 tatatggacg caataatatc gcccgattat tactatgttc ttaccattgc tggtcagtct
    12061 aatgctatgg cgtatggcga aggactgcca ttaccggcca gggaagatgc gcctcatccc
    12121 agaattaaac aattagcgag atttgcgcat acgcatcccg gaggcccgtc atgtcacttt
    12181 aacgacatta ttccactaac tcactgccca cacgatgttc aggatatgca gggttatcac
    12241 catcctctgg caacgaatca tcaaacacag tacggcaccg ttggccaggc actgcatatt
    12301 gcacggaaat tactgtcctt tattcctgat aacgcagggg ttctcatcgt tccgtgttgc
    12361 cgtggcggat cggcttttac cgcgggcagc gaagggacat attcagaacg gcacggagcc
    12421 agccatgatg cttgtcgttg gggaacggat actccgctat accaggattt agtcagcaga
    12481 acgcgagccg cactggcaaa aaatccgcag aacaaattcc tcggcgtatg ctggatgcaa
    12541 ggcgaatttg acttaatgac cagtgactac gcgtcacacc ctcaacactt taatcatatg
    12601 gttgaagcct ttcgtaggga tctaaaacaa taccattctc agcttaataa tattactgac
    12661 gcgccgtggt tttgcggcga taccacctgg tactggaaag aaaatttccc tcatgcgtat
    12721 gaagctattt atggcaatta tcaaaataat gttttagcta atattatttt cgtcgacttc
    12781 cagcaacaag gtgaaagagg actgacgaac gcgcctgatg aagacccgga cgatttaatc
    12841 atgggatatt acggttcagc gtaccgctca ccggagaact ggacgacggc actgcgaagc
    12901 agtcatttca gcgcggcagc ccgtcggggg attatttctg acaggtttgt agaagcaatt
    12961 ttgcagtttt ggcgcgaaaa gtgagcgttc attaatttga tttattcgca tccatcaacg
    13021 cttttaactc ctccggcgta aacggtggtt tgcgtcggtg caacatcgca tggcaattag
    13081 ggcacacaag cagcaaaaaa acaaatgaga tcaaggtaat atatcttcac ttgcaggctt
    13141 gaacagcaga aaatttgcga gcatgatcgc aattatttca ctgctaggga tatcaatggc
    13201 ttccagtaaa tcgctacagc aggcaattgc caatataaaa atctggcata agggtgaaca
    13261 gcgcgcaccg cataaaccat tgttattgct atacgtatta gcgggatacc tgaatggaca
    13321 tccacgcctt ttcgattatg gatcggaaat ctacgaacct ctgcacagtt tactggaacg
    13381 ttttgggcca cagcgttcac agtaccgacc tgatatgccg ttctggcgat tacaaggcga
    13441 cggattctgg caattacaca atgccgagct ttgctcaaca gcagggagca gccgacaacc
    13501 gccagtgaag gaactgaacg aataccacgt tgcaggcggt ttcgatgaac agcactacgc
    13561 cctggtaacc ggcaataaaa aactcatcaa tactctagcg caacagatac tcgaagcgca
    13621 cttcaccgag agtattcagg aagaaattgc tgacgagctg ggttttgatc tccagcaaat
    13681 ccgtaaacag cgcgacccgt tatttcgcaa aaatgtatta cgcgcttata actaccagtg
    13741 cgcgatatgt ggctttaata tgcgccacga cgacaccacc gtcgcgctgg aagcggcgca
    13801 tataaaatgg aaacagcacg gcggtccttg tgaaattcct aatggcctgg cgctttgcgc
    13861 aattcaccat aaagcctttg ataaaggatc gattggcctg gacgaaaata tgcgggtact
    13921 ggtatcagat gcagtaaacg gcggagggag cgttgagaga ttattctggg attttgacgg
    13981 gaaaattatt gcgttgccgc aggtgcgtaa aaattaccct tatgaggggt ttgtggagtg
    14041 gcatcggaat gaggttttta gggggtgagg ttgataacta tcgcagtatg ttcaatattg
    14101 ctgacataat cagtgcagga tcttgccgct agtttacaac tcgccttcga tagcggcgca
    14161 aaacgagttc tgttgccgat atcctgggct atggatattc caacagttcc ggcagagttg
    14221 tttaccaagt ttcaggtaag tttttactca gaaccggttg atgcggttta taaggcgctg
    14281 gatgtgaatt aacacattcc tcagcttatt tacaaaagga ttattcccat ttgatataat
    14341 at
//
LOCUS       DAJTNN010000100        14222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTNN010000100 DAJTNN010000000
VERSION     DAJTNN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.100"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            505..1329
                     /gene="gadX"
                     /locus_tag="OO150_002656"
     CDS             505..1329
                     /gene="gadX"
                     /locus_tag="OO150_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5516749.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1697..2425
                     /gene="gadW"
                     /locus_tag="OO150_002657"
     CDS             1697..2425
                     /gene="gadW"
                     /locus_tag="OO150_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5516750.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSLGTFS"
     gene            complement(2788..5901)
                     /locus_tag="OO150_002658"
     CDS             complement(2788..5901)
                     /locus_tag="OO150_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5516751.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OO150_002659"
     CDS             complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OO150_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR5516752.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7143..7421
                     /locus_tag="OO150_002660"
     CDS             7143..7421
                     /locus_tag="OO150_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516753.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OO150_002661"
     CDS             complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OO150_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5516754.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OO150_002662"
     CDS             complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OO150_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5516755.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9575..9907
                     /gene="hdeA"
                     /locus_tag="OO150_002663"
     CDS             9575..9907
                     /gene="hdeA"
                     /locus_tag="OO150_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5516756.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10023..10349
                     /gene="hdeB"
                     /locus_tag="OO150_002664"
     CDS             10023..10349
                     /gene="hdeB"
                     /locus_tag="OO150_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5516757.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10413..11060
                     /locus_tag="OO150_002665"
                     /pseudo
     CDS             10413..11060
                     /locus_tag="OO150_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(11102..11632)
                     /locus_tag="OO150_002666"
     CDS             complement(11102..11632)
                     /locus_tag="OO150_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5516758.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OO150_002667"
     CDS             complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OO150_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5516759.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12709..13826)
                     /locus_tag="OO150_002668"
                     /pseudo
     CDS             complement(12709..13826)
                     /locus_tag="OO150_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3946 a         3019 c         3156 g         4101 t
ORIGIN      
        1 attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa
       61 gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc
      121 tttaaacaca cctgataaca taacgttgta aaaaccgaat gcccagcctt taaaaaaaca
      181 gctgggcatt cggttgctta ttaatcgcaa taatatattg gctgtctatt catcgtgttg
      241 ataagatata tgttatatga atgtttatta aatgtagcga ttgcctttaa tgtcacgaaa
      301 agggattatt tgcttgctat taatttcact gcgttcttaa ccgcgttttt gtattataaa
      361 tccagtcatc ctgcccgtag aacatcacac attatcatcc tgttctcccg cttcgtttaa
      421 atttatttat caatcaattt gacttaagag ggcggcgtgc tacattaatt aacagtaata
      481 tgtttatgta atattaagtc aactatgcaa tcattacatg ggaattgtct aattgcgtac
      541 gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt taatggtggt
      601 gacctggttt ttgcggatgc aagccaaatt cgagtagata agtgtgttga aaattttgta
      661 ttagtatcaa gggatacgct ttcattattt ctgccgatgc tcaaggagga ggcattaaat
      721 cttcatgcac ataaaaaaat ttcttcatta ctcgttcatc actgtagcag agatattcct
      781 gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgcta
      841 cgtaaaagag cattaatctt tgcgttgtta tctgtttttc ttgaggatga gcactttata
      901 ccgctgcttt tgaacgtttt gcaaccgaac atgcgaacaa gagtttgtac ggttatcaat
      961 aataatatcg cccatgagtg gacactagcc cgaatcgcca gcgagctgtt gatgagtcca
     1021 agcctgttaa agaaaaaatt gcgcgaagaa gagacatcat attcacagtt gcttactgag
     1081 tgtagaatgc aacgtgcttt gcaacttatt gttatacatg gcttttcaat taagcgagtc
     1141 gcagtgtcct gtggatatca cagcgtgtcg tatttcattt acgtctttcg aaattattat
     1201 gggatgacgc ccacagagta tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg
     1261 gcggcaagta ttgttgcgca aggaaatttt tacggcactg accgttctgc ggaaggaata
     1321 agattataga attttactca gacataaaaa aaacctggca taggggaccg ggaagaggat
     1381 agtctgccgt ctccagacta ataaaccgct ataacactcc ctgttggcac gggaaacttt
     1441 gtgctctcag taagttaaat ataactttta ctggaaataa gatcagccat ttttttataa
     1501 acataagcta tacgctgtgc gaaaatatat tcgtgctgca tttacttatt atcaattaac
     1561 tgttatgcaa aactactttg tggataaatt ttggtcctac caaatctggc agtttttgcg
     1621 ctaagaaaca gtctggcaac atttcattag tatactgaaa ttgaaataat cgcagtatga
     1681 aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga
     1741 tatttatcat gaacagcata aaatatcgct gcataacgag agtatcgtgc tgctggagaa
     1801 aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga
     1861 gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca
     1921 tagcaaagac cgtctattaa ttaaccagtc accccccatg ccgctggtga cggcgatttt
     1981 tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc
     2041 ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag
     2101 cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct
     2161 gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga
     2221 tgaaaatacc tgtttcagta aaatattact cgcttccagg atgtcgatgg ccagacgatt
     2281 actcgagtta cgtcaaattc ctctgcatac tattgcagaa aaatgtggct atagcagtac
     2341 gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca
     2401 acattcgcta ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac
     2461 acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt
     2521 tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat
     2581 tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt
     2641 gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct
     2701 gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc
     2761 acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc
     2821 cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg caaacatccc
     2881 gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac
     2941 cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat
     3001 ccgcgccgct tcgatgattg cccctatcgg cgttttccct tctttctgca tcatctcaac
     3061 ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc
     3121 aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac
     3181 gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc
     3241 ggcgagggcg aggaacacca cgaccagtga aatggcatac agtgcaggag cctggtttga
     3301 ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt
     3361 agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg ccgccgcttc
     3421 acctaaaatc tccattgacg ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt
     3481 ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata
     3541 ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt
     3601 tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat
     3661 tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt
     3721 cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga cctggtcagg
     3781 cgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag
     3841 gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc
     3901 gggtaagttg aacgggaaga cgacggcttt attgatactg cttaacgcaa tcattgcccg
     3961 ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact
     4021 gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa
     4081 caccgactga acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac
     4141 tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc
     4201 ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc
     4261 gcaaatcagc aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct
     4321 atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt
     4381 aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag
     4441 gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg
     4501 gtagatctcc ccggttgcgc cgctcataaa ggccatcggc ataaacactg cggaaagaac
     4561 aacagcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt
     4621 cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc
     4681 atccaccagt aaccctatcg ccagcaccat cccgaacata gttaacgtgt tgatggtgaa
     4741 accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg
     4801 gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat
     4861 agcctcaacc agtgttttaa aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc
     4921 gtaaggataa accgtcttca gacttgcggg gaaataggct gataagcggt tcagctcctc
     4981 tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc
     5041 ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgctc caagttcgac
     5101 gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt
     5161 gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg
     5221 gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt
     5281 aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag
     5341 ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg
     5401 gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct
     5461 gccgttatca gaaataaacg ccgctaccat cagaatatta ctgctcgact tatcaacgct
     5521 aatcccctgc tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg
     5581 cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc
     5641 attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt
     5701 ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac ctggataggt
     5761 agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat
     5821 gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg
     5881 atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc
     5941 ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg
     6001 ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg
     6061 gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acaacatcgt ctttatccag
     6121 aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa
     6181 tacattctgg cggctacctt catccactaa tgccgtgacg tacatgccag gcagcaagtc
     6241 accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catccaccgt
     6301 cgggtcggag aatttcagtg tgccggtctg gctgtagcgt ttaccatttt ccagattgag
     6361 ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat
     6421 gcgcaagaaa tcctgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac
     6481 ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt
     6541 cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg
     6601 ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc
     6661 ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa
     6721 ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt
     6781 tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt
     6841 atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc
     6901 aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccggggga
     6961 gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc
     7021 gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt
     7081 cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag
     7141 atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca
     7201 ggcacagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag
     7261 ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata
     7321 aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc
     7381 ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata
     7441 cccagggtga cgatgtcgct catacgtctt agtttcaact tgtacataat gttgaatttc
     7501 tgaatcttta atgtttgttg cgaaatattg agcgtcaacg tgatattgct ttcattttta
     7561 tttttcagaa tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc
     7621 ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg
     7681 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
     7741 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
     7801 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
     7861 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
     7921 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
     7981 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
     8041 tataagaact ttacccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
     8101 ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg
     8161 taaatagtaa acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa
     8221 ccagtttgtg atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg
     8281 agtctccttg tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga
     8341 aattacctga gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac
     8401 ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt
     8461 aaccgcccat agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa
     8521 aacattaaac ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc
     8581 catggagtga aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt
     8641 atttcaatga tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg
     8701 atcatagtca catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta
     8761 acgaacaaac tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc
     8821 agtgtggaaa ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg
     8881 acgatatcca gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga
     8941 taccaactca tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca
     9001 atggcaaaaa tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg
     9061 actgcgacga ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac
     9121 ccgacaataa gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata
     9181 tcgccagaga cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca
     9241 ataaactgga ttgctctgcg atgtttttta agcatctcca gatcaaactt caaaattgtt
     9301 gccttatcta tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat
     9361 caatctatgc caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc
     9421 agtatctgat gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt
     9481 ccatcaacat gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgatttgtg
     9541 acggctcttt cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct
     9601 tggtggtctg cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga
     9661 taacaaaaaa ccggtcaact cctggacctg tgaagatttc ctggctgtgg acgaatcctt
     9721 ccagccaact gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc
     9781 ggttttagat gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca
     9841 ggataaacaa gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga
     9901 tatgtaattc cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat
     9961 cttctttaaa agcagctatt cctcctgttc atatataatc tatatattga atgggttaca
    10021 aaatgaatat ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt
    10081 cactggtgaa cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc
    10141 aggaatttat tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg
    10201 aagaaacagt atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa
    10261 ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa
    10321 atcaagcatc taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg
    10381 agccatcttg ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta
    10441 tactcgcggc aattgcctgt ggcgctattg gcatggaaag gcaaatgtga ggcaaaggag
    10501 cagggttacg cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga
    10561 aatatggttt tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg
    10621 ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc
    10681 aaaatattgt aggtttgacg acggcagcgg atatctggat gaccgccgct ataggtatgg
    10741 ttattggcag tggtatgtac gaattgggta tgtatggttc agtgatgacc ttgctggtgc
    10801 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
    10861 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
    10921 cggatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
    10981 ttcatgccac aacgtcgata gaagatctgt tgcgattatt aaaaggtatg gcaggggtga
    11041 aaggcgtttc aattagctaa tctgtgtggc agcgtagtca gactcaccgt aagcctgaaa
    11101 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
    11161 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgttatt ttcagtttct
    11221 ccagaatatc ctccgttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
    11281 gcgaaagtga cacagccgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
    11341 aatcgatgat agtgatagtc cgcggaacaa aggtcaccgg cactttcccc aggcagcgtt
    11401 taatattaaa gggggagaaa ataatcacat ggacaggttt taatcgttca atttgagtga
    11461 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacaaag gcattctgat
    11521 gcaacattcc gtcgatctct tcttcgttct gtatatgaac gacattacct ttgctcaaaa
    11581 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
    11641 cctggtgcgg acattaattt tataatcagt ttgccaaagg taattaacag gacagggctt
    11701 ataataataa tagttagctt gctgtgtatt aattttatgt aatgcacgat gcagatgctg
    11761 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
    11821 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
    11881 atcccatagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
    11941 catattcact tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg
    12001 aatggtaccg aggatggtaa caaagtgatt acgatagttc actgggtcaa ggaagccgct
    12061 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggtttcg catagctatc
    12121 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
    12181 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
    12241 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
    12301 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
    12361 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
    12421 tgaggcgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttttgtttag
    12481 ttcaattcca ttgattttgg ggcgtggcat aagtatccat cgccccataa tataatcctt
    12541 tgttttaaaa ttacataaaa tctattttta ttcaataatt ataaagtgct aataatcaat
    12601 attatggtat tatattctga taatgatttg ataagcgaat tcttttgcca gatggtattt
    12661 ctatttccac cccataattt atatcttttt cattaggaag tggcctcttt aaataaggcg
    12721 tgtgtgacat tttttttggg ttaagcgtct cgaattgaaa tacttcgaac tgatattcag
    12781 acatttctgc ccatgtttga attttattgt atcgagagag tgtcgtagag gctttaactt
    12841 gcttataaca ttgtttttgg aattcttgct gatattttaa tatgtcattg tatgaaagat
    12901 tggttatcct ggactctaaa aatttaatta ttgtcgaagc aatttccgct tttgctcgta
    12961 aaaagttgtc attaatggtt aataatgttg aaaataaacc ttcgatttgt ttgtcaagat
    13021 attgtccatc tttatataat ttgggggggt agggtgttct ttatgtaaaa aatagtgtca
    13081 cctaaatttg cggcagtgaa atttatatta agtagatctt cgaaataaaa ccttgacaag
    13141 gttagagcga acccggaaag gttctgtcct gtcatattga aatcaataaa gttggttccg
    13201 gcaaagactt tcatcctgtt tttattttca ctgtgctcaa tatataactt cgtcattttt
    13261 tgatacaggc tttgaatatc ttttatatta gttggtaagt cagctcgttg ttcagcaata
    13321 aaattagtat ctgaaaatat ggtttggaca ctgaatgtta tgggtattcc ctcttctacg
    13381 ctaattttag ttatgattgc cttgtcgggt ttaggattag cgacaacaat gattcgacat
    13441 tcagcacttt tgtttttttc atatctaatg ttattaatct gattaagtat tgattgtgtc
    13501 tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat taataagact tacaagttga
    13561 accacaactt ttgagtagtc tgcatttgta tatgtggtgt tcgtgtggca tgcattaatt
    13621 tgttggagtt gttgttgcgc taaatcaaat tgttcagggt tgtatccttc tccagagaat
    13681 gtggaattaa tgggttgttg ttcttcccaa ttatcacaag gccacttcgg tgtgtctggc
    13741 gttttaacat tatcggtatt tgttgtgcag ttataaatat tgccgtgata tgtatttatt
    13801 atacctgggg taaagtcaat tgacataaca ttccttattc atcatctgtt ttatgctaag
    13861 gtttttaaat tggataatta gaacaggaat ggattttctt ctacacacaa taaatataat
    13921 gcgttatatc ttcctgatat aattaccttt aaattccctt tcatttccac ccggtaatat
    13981 ttgtgtataa aaaaagcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag
    14041 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
    14101 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
    14161 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
    14221 ta
//
LOCUS       DAJTNN010000101        14097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTNN010000101 DAJTNN010000000
VERSION     DAJTNN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.101"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..386)
                     /locus_tag="OO150_002669"
     CDS             complement(<1..386)
                     /locus_tag="OO150_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5516760.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSAT"
     gene            complement(774..1520)
                     /gene="cutC"
                     /locus_tag="OO150_002670"
     CDS             complement(774..1520)
                     /gene="cutC"
                     /locus_tag="OO150_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5516761.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1534..2100)
                     /locus_tag="OO150_002671"
     CDS             complement(1534..2100)
                     /locus_tag="OO150_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5516762.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            2316..4049
                     /gene="argS"
                     /locus_tag="OO150_002672"
     CDS             2316..4049
                     /gene="argS"
                     /locus_tag="OO150_002672"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5516763.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            4226..4714
                     /locus_tag="OO150_002673"
     CDS             4226..4714
                     /locus_tag="OO150_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5516764.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDIEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4834..5226)
                     /gene="flhe"
                     /locus_tag="OO150_002674"
     CDS             complement(4834..5226)
                     /gene="flhe"
                     /locus_tag="OO150_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5516765.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5226..7304)
                     /gene="flhA"
                     /locus_tag="OO150_002675"
     CDS             complement(5226..7304)
                     /gene="flhA"
                     /locus_tag="OO150_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCR5516766.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGCEQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(7297..8400)
                     /gene="flhB"
                     /locus_tag="OO150_002676"
                     /pseudo
     CDS             complement(7297..8400)
                     /gene="flhB"
                     /locus_tag="OO150_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(8483..9127)
                     /gene="cheZ"
                     /locus_tag="OO150_002677"
     CDS             complement(8483..9127)
                     /gene="cheZ"
                     /locus_tag="OO150_002677"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5516767.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLAEARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(9138..9527)
                     /gene="cheY"
                     /locus_tag="OO150_002678"
     CDS             complement(9138..9527)
                     /gene="cheY"
                     /locus_tag="OO150_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5516768.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9542..10480)
                     /locus_tag="OO150_002679"
     CDS             complement(9542..10480)
                     /locus_tag="OO150_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HCR5516769.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFG
                     MPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI"
     gene            complement(10483..11295)
                     /gene="cheR"
                     /locus_tag="OO150_002680"
     CDS             complement(10483..11295)
                     /gene="cheR"
                     /locus_tag="OO150_002680"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5516770.1"
                     /translation="MRLPGCSRYSFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
                     LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYR
                     VWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNL
                     TPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMI
                     YFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            complement(11433..11999)
                     /gene="flhC"
                     /locus_tag="OO150_002681"
     CDS             complement(11433..11999)
                     /gene="flhC"
                     /locus_tag="OO150_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5516771.1"
                     /translation="MSEKSIVQEARDIELITLGARLQMLESETQLSRGRLIKLYKELR
                     GSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQC
                     PQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPS
                     RAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(12002..12352)
                     /gene="flhD"
                     /locus_tag="OO150_002682"
     CDS             complement(12002..12352)
                     /gene="flhD"
                     /locus_tag="OO150_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5516772.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVNDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13130..13558
                     /gene="uspC"
                     /locus_tag="OO150_002683"
     CDS             13130..13558
                     /gene="uspC"
                     /locus_tag="OO150_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR5516773.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13565..>14097)
                     /locus_tag="OO150_002684"
     CDS             complement(13565..>14097)
                     /locus_tag="OO150_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCR5516774.1"
                     /translation="TSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDR
                     KLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALI
                     VNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRS
                     AESQQRDKVATFPKLA"
BASE COUNT         3629 a         3732 c         3287 g         3449 t
ORIGIN      
        1 cacgttgcag agtcagttgc ttttaattct ttccatactg tttcggccat ttcctggcga
       61 tgagtttcga acttatcgtc actgtctatt ttgccgctaa caaattcatg ataaatatct
      121 ttagatgctt ttaatttagc aaataaataa tccatccctg actttggaat atggcaatct
      181 gcacattccg cacgtatccc tttctggttc gaaaagtgga cagttccctg atattcctca
      241 aaaggtttac tcatcgagtg gcaggaaagg caaaatgctg tatccgacgt tttatgtaag
      301 gctttttgcg ccagcaataa cccgccgcca ccaaccacaa ccgctatcag caaaaacaat
      361 aacccaatgc gttttttccc tcgcatagat tcctctaaat aaattccaga acaccgaaat
      421 cattggataa ttaaatacac aacgaattaa ccgcttgcat aactcggcac aacactgcct
      481 gaaataatcg ataaagaata tgatttatta caatgtaatc attaattgct aaggaataac
      541 cctgttgtta tttagaatat atatttgtca gttttagcaa tttaaacccc ataattaatg
      601 tgaatatata aaatattttt agcacaacgc tatgttcact gtcacccgct caacagatca
      661 cggttatcgt acgtttttta tactgctcag ggataaaaaa aggcccctgt cgaaattgca
      721 ggggcctggt acgagcaagc atcatattgg gcgacatgat gcaacggtaa aaatcatttg
      781 gcctgatggc gttcaatgat tcctttcatt tcagcaaccg ccgccccgtc tacgacataa
      841 cgcgaatact cgtccgcgtg tgcatctgat gacatggaca atccttgatt acgataacgc
      901 atcggtgaag cttgccacgc tcccgcggag ctatggactt ccagcactcc ggcatcgagg
      961 aagtggtgca ggttttctgc acggactcct gctccggcca taatgattgg agcatcacga
     1021 tgggcaataa gttccataat ttttgataaa ccttgcagtg catctgattt ttgccctgat
     1081 gtcagcaccc gggcgatgcc caattccgca agattactga gtgtatttaa agggttagcg
     1141 cacatatcga aggcgcgatg aaaagtcact gccagcggac cggcagcagc cattattttt
     1201 tccattcgtg gcatatcgac attcccgtca acatcgagca cgcccgtcac cagtccagga
     1261 aaacctaatt cgcggactgt gcgcacatcc tcaagaatgg cggcaaactc accgtcgctg
     1321 tagcaaaaat caccaccgcg tgggcggata atcggatgca cagggatcgt cacccactgg
     1381 cgcacggatt tcagtacacc caacgacggc gttaagcccc cctcttttgg ggctgcgcat
     1441 aattcaactc tgtctgcgcc gttttgctgc gccgttagtg cacattccat gctgtaacag
     1501 caaatttcca gtaatgccat ttttactcct taattacgcc gactgctcgc tggcaacgat
     1561 ctcttcaatg gaccacggat gaaacttaat agttgttttg ccatcagtga ccgccagcgt
     1621 cggattcggt aatcgctcat actcaccttt tggcgaactg gttttcactg agatccccgg
     1681 taggctgagt ccttcatcag aaagcaatgc cagcgcacgg gcattcagcg tttctggatc
     1741 acccggcaga acgatttcaa tatgttccca accttcgtgt gggtaacgtt tttccccggg
     1801 ccacggtagc tccacaatgg aaaactgcca gtgcgcaacc tgtaccggtt catgcaattt
     1861 aaacagacaa atcggtctgc cattgatcat attttctgac aaaagctcgc cacactgttc
     1921 aaaaccgcga cgccagcgct cagcggtgac gttttgatgg caacgcaaag aaatgtgatc
     1981 ggcagtcagc ggagtgatat ccaaccccag acggcgggag agttcatcta atgcgtgggt
     2041 aaatcgcggt aaatccgatg caatatcctg cagctcgtcg atagattgcc agttcgccat
     2101 aatcactctt cgtctttcag taaaagcgtt aatttaccct gttgccctgc gccaaccaac
     2161 cgctgatttc acgccgcttc tgatgcaata gtgaaaacgg caatacgcca cgcgcacgtt
     2221 gctgacgaaa acagccattt gcagtatact cccgccctaa tttctttaac tggtgcgggc
     2281 aatttttgct cgcttcatca atgtaaggta ttccggtgaa tattcaggct cttctctcag
     2341 aaaaagtccg tcaggccatg attgcggcag gcgcgcctgc ggattgcgaa ccgcaggttc
     2401 gtcagtcagc aaaagttcag ttcggcgact atcaggctaa cggcatgatg gcagttgcta
     2461 aaaaactggg tatggcaccg cgacaattag cagagcaggt gctgactcat ctggatctta
     2521 acggtatcgc cagcaaagtt gagatcgccg gtccaggctt tatcaacatt ttccttgatc
     2581 cggcattcct ggctgaacat gttcagcagg cgctggcgtc cgatcgtctc ggtgtttcta
     2641 cgccagaaaa acagaccatt gtggttgact actctgcgcc aaacgtggcg aaagagatgc
     2701 atgtcggtca cctgcgctct accattattg gtgacgcagc agtgcgtact ctggagttcc
     2761 tcggtcacaa agtgattcgc gcaaaccacg tcggcgactg gggcactcag ttcggtatgc
     2821 tgattgcatg gctggaaaag cagcagcagg aaaacgccgg tgaaatggag ctggctgacc
     2881 ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga agatgaagag tttgccgagc
     2941 gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga atatttccgc gagatgtggc
     3001 gcaaactggt cgacatcacc atgacgcaga accagatcac ctacgatcgt ctcaacgtga
     3061 cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa cccaatgctg ccaggaattg
     3121 tggcggatct caaagccaaa ggtctggcag tagaaagcga aggggcgacc gtcgtattcc
     3181 ttgatgagtt taaaaacaag gaaggcgaac cgatgggcgt gatcattcag aagaaagatg
     3241 gcggctatct ctacaccacc actgatatcg cctgtgcgaa atatcgttat gaaacactgc
     3301 atgccgatcg cgtgctgtat tacatcgact cccgtcagca tcaacacctg atgcaggcat
     3361 gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt accgctggaa caccacatgt
     3421 tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac ccgcgcgggt ggtacagtga
     3481 aactggccga tctgctggat gaagccttgg aacgtgcacg ccgtctggtg gcagaaaaga
     3541 acccggatat gccagctgac gagctggaaa aactggctaa cgcggttggt attggtgcgg
     3601 tgaaatatgc ggatctctcc aaaaaccgca ccacggacta catcttcgac tgggacaaca
     3661 tgctggcgtt tgagggtaat accgcgccat acatgcagta tgcttacacg cgtgtattgt
     3721 ccgtgttccg taaggcagaa attaacgaag agcaactggc tgcagctccg gtaatcatcc
     3781 gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa accctcaccg
     3841 tggttgcccg tgaaggcacg ccgcatgtga tgtgtgctta cctgtacgat ctggctggtc
     3901 tgttctctgg cttctacgag cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta
     3961 acagccgtct gaaactggca caactgacag cgaagacgct aaagctgggt ctggatacgc
     4021 tgggtattga gacagtagag cgtatgtaat cgatttttcg tgagagtaaa gcctgatcag
     4081 ggttacctga tcaggctttt ttattgccat ctaaatgtat tctgaaaatg gacatgccat
     4141 tgttttctga ctgttggata agaggccaga agcgtaatat ccggccccag ggaaacgata
     4201 acggttgaat ttaaggaata ccgcagtgtt taaatttctt gtattaacat taggcattat
     4261 ctcttgccag gcttacgcag aagatacggt tatagtaaac gaccatgaca tttcagccat
     4321 caaagattgt tggcaaaaaa attcagatga tgatactgac attaacgtga tcaaatcatg
     4381 cctgcgacaa gaatacaatc tcgtcgatgc gcaattaaat aaagcctatg gtgaagctta
     4441 tcgttatata gaacaagtgc cacgcacagg tgtaaaaaaa cctgatatcg aacaacttaa
     4501 cttgcttaaa aaatcacagc gagcctggct ggattttagg gacaaagaat gtgaattaat
     4561 cctttcaaat gaggacgttc aggatttaag tgacccttat tctgaatcag aatggctctc
     4621 atgtatgatc atacagacca atacgcgaac tcgccagttg cagctatacc gtaactctga
     4681 agatttttat ccaagccctt tgacaagagg ataattcaca tctttttggc atgttttgtt
     4741 gcaagctatt cctgataaat aattgcaaca agacatcgag cctttttcac tgagttatta
     4801 aacatactcg cgagcgcgta atttttttgt ccttcagcga taattcacaa tcacttcatt
     4861 acgttgtacc tttagcggtg gaattaaccg cccaccgcct ggcacttccc agataaatcg
     4921 caacggttct gctgccgcta taccggaaaa ggccacggtg gttccgctct gcccctctaa
     4981 ttcgacacaa cgagattgcg aacacaagcg aacccgcagt cctgaaggtg tcgggccgat
     5041 aagctgataa cgccacgcta ccagcgtcat caatcctgaa gcaggttgtc gcgtagaaag
     5101 aggcgcagac gacatcgact caccgcgatg atttagcgta attcctacac tacttgcctg
     5161 ccacatcccc tccccggcgg cttgcaccag cagcggaaac aataatattg ctaataaggt
     5221 tctcattatt tgccgccaat tgtcgccgtc atgcggatat gtcggttatc agacagttcc
     5281 agattcgaca ggaccactaa ctgcggcaag ctgcggcgca ggaagcgaga caataatggt
     5341 cgcagcgcgt ggttcaccaa caatactggc ggcgcaccca gcatctcctg acgggatagc
     5401 gcttcctgag tttgcgccag taaacgatcc gccagccctg gctccagtcc tcccccgccc
     5461 tgcagcgcct gtagtaacaa acgttccagc ggtgtatcga ggccaataac atggacttca
     5521 tctttgccag gaaaccactg ctgggtaatc gcccgtccca acgccacgcg cacgacggcg
     5581 gttaattcat gtggatcgct ttggatgggc gcatgttccg ccagcgtttc gagaatggtg
     5641 cgcatatcgc gaatcggtac tttttcatcg aggagatttt gcagcacttt atgcagtgtg
     5701 gtgagcgtga cgacgccagg aacgagatct tccgtcagct ttggcatctc ctgggcgacg
     5761 cgatccaaca gctgttgcgc ctcctgacga ccaaacagct ctgcggcatg ctggctaatg
     5821 aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa ccactgtgta cccctgaatc
     5881 tgcgcctgtt cttttagcgc actttcaatc cagatagcat tcaggccaaa tgccggatcg
     5941 acggtcgcct caccaggtaa cgtcccggca gcggttccag ggttaatcgc caaccagcgc
     6001 cccggataag catcaccact gccaatctcc acgcctttca tcaaaatgcg atagcgggca
     6061 ggttgcagat ccatattgtc gcgaatgtgc accactggcg gcagaaatcc catctcctgg
     6121 gcaaatttct tgcggatact gcgtatacgg cccaacaact caccatcctg ctggaaatcg
     6181 accatcggga tcagtcgata acccacttcc attcccagag aatcttccag ttgtacatcg
     6241 ttccacgtcg cttcgacaac ggtattattc tctgccattt ttaccggttt gggttcggca
     6301 ggcgcttttt gttcgcatcc gcgtatccac caggccagcc ccagcaatcc ggcagtgaac
     6361 agcaaaaata ccaggttcgg cattccaggc accaagccga gtaaaccgag cacggcggcg
     6421 cttaacaaca taacgcttgg gttactgaaa agctgattca ccatctgctc gccaacatcc
     6481 tgatcggtgc tgacacgcgt aacgatgacc cccgcggcgg tagaaatcac cagcgccgga
     6541 atttgtgcca ccagaccgtc gccaatggtc aatagcgtat aactttccgc cgcgtgtccc
     6601 atgctcatgc catgttgcag cacgccgacc agcaacccgc cgacaacgtt aatgaccatg
     6661 atgaggatcc cggcgatggc atcgccgcga acaaacttac ttgccccgtc cattgagccg
     6721 taaaaatcgg cttcctgagt cacttcggag cggcgttttt tcgcctcatc ttcaccaatc
     6781 aatccagcgt taaggtcggc gtcaatcgcc atctgcttac ccggcatacc atcgagaaca
     6841 aagcgcgcac ccacttctgc gatacgcccg gcacctttgg taatgaccat aaagttgatg
     6901 atcacgagaa tgacaaacac cacgatacct atagcgaaat tgccaccaac gaggaagtga
     6961 ccgaacgctt cgaccacctt ccctgccgcc gccgcgccgg tatgcccttc catcaaaatg
     7021 atacgggttg aagccacgtt aagtgccaga cgcaacaacg tagtaaacaa cagaatggtc
     7081 ggaaacgcag caaactcaag cgtgcgctgg gtaaacatcg ccaccagcaa caccatgatc
     7141 gacaaggcaa tattgaaggt aaacaacagg tcgagtatga atgcgggcag tggcagcacc
     7201 atcatcgaca agatcaacag gatcaaaatc ggtccggcaa ggatctgcca ttgtgtcgat
     7261 ttcaggtttg cgggcaggcg cagcatcgcg gccagattac tcatgggtcg gtttctcgtt
     7321 aataaaatcc agggcttccg gcaccggaag atgagtaggt tgtacagggc gctgtccacc
     7381 agccagacgc cagcgtttca gttgccagat ccaggccagc acttccgcca ccgcggcgta
     7441 cagttgaccc gggatttgtt gaccaatctc cgcatgtcga tacagcgctc gcgccagcgg
     7501 cggcgcttca agcgtcggga cgttattttc agcgccaatt tcacgaatgc gcagcgcgac
     7561 cagccctgca cctttagcga ccactttcgg tgcgctcatt ttgttttcgt catactgcaa
     7621 cgctaccgaa tagtgggtcg ggttattgac aatgacatcc gctttcggca catcggccat
     7681 catccgacgc cgtgcggcag ctcgctgcat ctgacggatc cgtcctttaa catgggggtc
     7741 gccttcgctt tgtttgaact catcacgaat atcctgccgc gacattcgca gctttttcag
     7801 gtggctgaag atttggaaaa agacgtcaaa tcccaccatt ggaatgacac caagcaccac
     7861 cagcagtgcg catagcccta ccaaatccat cgcattaccc atggcggtaa tcggagactc
     7921 ggccagtaag cgcatcatct gcggccagtg atgccagaga aaaaaccccg tcacgctgcc
     7981 aaccaagatg gttttcaaaa ttgctttaag caactccgcg ccagtctgtg ccgagaacat
     8041 ccgtttaatg cccggtagcg ggttgagttt ggaaaacttc ggctgcaagg attttccgct
     8101 aaataccagc ccccccagca tgaccggaga aatgagcgct accagcacca cgccgctaat
     8161 cagcggtagc agcgccagca tggcttctct gatcagcaga ataatctgcc cgaggatcag
     8221 attcgggtct ttgataatac tgtgatcaaa atgcagccca gcggagagca tgcccgacaa
     8281 tcgacgggcc agcgacacac cgccgaacca gataacacac acgcccacca gcaaaatcag
     8341 cagtgaggtc agttcacggg aacgcgggat ttagggcttt tgctgccaca catcaagcat
     8401 agtgtgcgtt tgtcggatgc ggcgtcatcg ccttatcaga ccgcctgata tgacgtggtc
     8461 acgccacatc aggcaataca aatcaaaatc caagactatc caacaaatcg tccacctgat
     8521 cctgactggc taccacaccg gctttgctgg tatcgacctg aggtccatta agcaaactct
     8581 ggttttcacg ttttggacgc gactcctgtt ccgggatgtt ttccaacagc accatcagca
     8641 actggcgttc gatctcctga atgacatcca tcatccgctt aataacctgc ccggtgagat
     8701 cctgaaaatc ctgcgccatc atgatttcca gcagttgcgc gttagtaaag ctggtatgcg
     8761 cgggtacatc tgccagaaat tgtcgtgtat ctgttaccag ttcacgggcc tcggcaaggt
     8821 caatcggatc ggcaaaccag tcatcccaac gttgggttaa cgcttttgct gatttctcca
     8881 tttgatcctg atgcggttgt gacgcctcaa cactgttcag cgcccgctcc gcagcctggg
     8941 cggtcatctg cacaacatag tacaaacgat cgcgcgcatc ggggatggct tccgccgctt
     9001 cggcaatggc ctgatccagc cccagttccc gcaaactgtc gcgcagcata cgcgtcaggc
     9061 tgccgatgcg cgcaatgata tcgccagctg aatgctcgtc agcaggtttg attgatggtt
     9121 gcatcatagt cgcatcctca catgtccagt ttatcaaaga ttttgttgag tttttcctcc
     9181 agcgtcgcgg cggtaaatgg cttcaccaca tagccactgg cccccgcttg cgccgcagca
     9241 atgatgttct ctttcttcgc ttctgcagtc accattaaca ctggcaatgc cgacatcgcg
     9301 ccatccgcac gaattgtttt cagcaattcc aggccatcca tattgggcat gttccagtcg
     9361 gagataacaa atccataacc gcctgcctgc aacttattga gagcgtcgac gccatcttcc
     9421 gcttcctcaa cattattgaa tcccagctct ttcagcaggt tacgcactat gcgtcgcatg
     9481 gtggaaaagt catccacaac caaaaattta agttctttat ccgccatttc acactcctga
     9541 tttaaatacg tatcgcctgt ccggcactaa tttttgccaa catttgctgg cttacctggc
     9601 taagatcgac cacttcgcag acaccaccca tattgatggc ctcgcgcggc atgccgaaca
     9661 ccacgcaact tgcttcgttt tgcgcaaggg tccatgcccc cgcctgacgc atcgccaaca
     9721 ttcccaccgg gccgtcgtga attttgattt gataatttgc gccactacgc gccagttcca
     9781 tatgccgatc gcccggcgca atataggcat gccccggcaa gacacgttct ccgtcttcgg
     9841 cttctttaac cccgatctgg caaagcttat taagtctgtc ggcaaaagag cgggtgaaac
     9901 cgggcggcat atgttgggta attaacaatg ccgggctgga aagcggcaac ggttgcagta
     9961 cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt ttttcagaac
    10021 tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa tggcttatgt gctgcaaggc
    10081 tcgcctttgc tgccgtacgc accttttcag caatcatttc gctatacgcc agcattcctt
    10141 cgcgaatacc cagttgcggt ttggtgacaa aatctattgc ccccagctcc agcgcgcgca
    10201 gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat cacaacgggc attggacgca
    10261 aacgcattaa tttttcgagg aaatccagtc cgtccatccg cggcatttca acatccagcg
    10321 tcagcacatc gggattgaat ttcttaatca agtcacgcgc gaccagcgga tcaggcgcgg
    10381 tcgccaccat ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc tggcgcatca
    10441 gtgccgaatc atcgacagat aacaccctga ttttgctcat cgttaatcct tacttagcgc
    10501 atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg tggctaaagt tttcagagtg
    10561 acccgcaaac agcaatccgt cgggtttaag gagcggaaca aagcggcgca aaatctcctg
    10621 ctgggtattt tgatcgaagt agatcatgac gttacgacag aagatcgcat caaacggccc
    10681 cggcacggtg tactgtttcg ccagtagatt cagcggggca aaatcaacat agttcgccag
    10741 ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc cctcgcatga aataccgttg
    10801 cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga taaataccgc ttctggcttt
    10861 ttccagcact tcggtgtcga tatcactggc aaacactttc cagcgtccgg gcgcggtgcc
    10921 caatgtgtca gccagcgtca tcgcaatgct gtacggctct tcgccggttg aagccgccgc
    10981 gctccatacg cgatactcgc cagaacggcg acgtgcgtga tccgcgagca gagggaaatg
    11041 atgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa acgcctgcca
    11101 ctcaccgctg tgctgattag attccagcaa gttcagataa tgaccgaaat ccgtcagtcc
    11161 cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat gatcggccag
    11221 gacgatcccg gctcgttgat agatcaattg acttatccgc cgaaaactgt accgagaaca
    11281 accaggtaac ctaataagat aagcacgaca tcatccttcc actgttgacc atgacaggat
    11341 gttcagtcgt caggcgttaa cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac
    11401 cgctgctggc atgttgcgcc acaccgtatc agttaaacag cctgtactct ctgttcatcc
    11461 agcagttgtg ggataatatc ggcaggattc tgggaaagtt tacgtctttt tactgcccgg
    11521 gatggcggtt gacataagct gcaggcaaag ctgccaacag gctggtgagc gtgggtaata
    11581 aaattgccgc cgcagcagtt gcagctggaa agttgcagta atccactttc aacaaaccgc
    11641 accaatgtcc aggcacgggt taatgccagc agtggtcctt cttctgcttg tgggcactgt
    11701 tcaaggtata aacggtaggc tttgatcacc gcatcgacgc cattacacaa acctgttttc
    11761 agtaaaaact gccatgcatt acagaacatc gaagcatgaa cgttttgttc ccaggtcata
    11821 aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg ggcttccgcg cagttcttta
    11881 taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga
    11941 gcgcccaggg tgatcaattc aatatcccgc gcttcctgaa caatgctttt ttcactcatg
    12001 atcaggccct tttcttgcgc agcgcttctt caggctgatt aacatcattc agcaagcgtg
    12061 ttgagagcat gatgccggta tgaatttgct ggagatcgtt aacgcgggaa tcttgcgtca
    12121 actgagtaat cgtctggtgg ctgtcaaaac ggaagtgaca aaccagttga ttggtttctg
    12181 ccagcttaac catttgcgga agagtcagtg ccgctaacgt tgtcgccatt tcttcattta
    12241 tgccgagacg aaacatagcg gacgctttgt cctgaacaat caaacgctgt gcaagtagta
    12301 aatatgacaa gttgatgtca taaatgtgtt tcagcaactc ggaggtatgc attattccca
    12361 cccagaataa ccaactttat ttttatgcgg tttcaccgca ccccgtgatg tcgccgggaa
    12421 gccccggtaa aaaataatta gcattagaat agtgcgataa gctgcaataa gcagaaccac
    12481 ctttttggtt taatatgtcc ttacaaatag aaatgggtct ttacacttat ctaagatttt
    12541 tcctaaatcg acgcaactgt actcgtcact acacgcacat acaacggagg gggctgcgat
    12601 tttcaataat gtgtgatgca gatcacacaa aacactcaat tacttaacat aaatgtataa
    12661 gccatacttt tgttttgggt gtatttcaat ctgttaaaaa gtttttcgct acgctagcaa
    12721 gcaaaaatga aactggaata atcgaaatgg gatgttgcgc acagttaaaa taactcaccg
    12781 taaataatca tctgctataa ataatcactt tcatgcaata ccagataagc tatttttaaa
    12841 cagacactta ccgcacaaca aactaattaa atacaaagaa aggcaacaat caacttaatg
    12901 ttaatgaaat gttttacata tattaaccac ttgtttaatg cggagaatca gtacactatt
    12961 ttcttataat tacatttgaa attatatgat caccgggagt gattgattaa ttgtctttac
    13021 taataatcag actaatattt acctgtttga ccgagttggg attgcgtcgt ttctccatta
    13081 ggagtaaagc tttaatgtca cctgaagttc acagaataag gaacaggtta tgagctatag
    13141 caatattctt gtcgctgttg cggttacacc ggaaagtcag caactgctgg caaaagcggt
    13201 atctatcgcc aggccagtaa agggacacat cagtttaatt actctcgctt ccgacccgga
    13261 aatgtacaat caattagccg cgccgatgct tgaagattta cgcagcgtga tgcaagaaga
    13321 aacgcaaagc tttcttgata agttaattca ggatgcgggg tatcccgttg acaagacgtt
    13381 tattgcctac ggtgagttaa gcgaacatat tctggaagta tgtcacaaac atcatttcga
    13441 tttggtgatt tgcggtaatc acaatcacag tttcttttcg cgagcatcct gctcggcgaa
    13501 aagagtgatt gcctcaagtg aggtcgatgt gctgttagtt ccacttacgg gtgattaacc
    13561 gctcctacgc aagctttgga aaggtagcaa ctttatcgcg ctgctggctt tccgcgcttc
    13621 gcggaactat ctgctttagg tcgctaatga agcactcctg ccagtggtta atatcgtttt
    13681 tcacgataac gtccagcatt tctgcatgac gggaaatacg ttccgccagc gacatagtca
    13741 atgcacgatc cagcgcagct gcaacttcgt cacgatcgta ggggttaaca attaacgccg
    13801 acgttaactc gtttgccgct cccgcaaatt gcgaaagaac aagaacgccc gggttggctg
    13861 ggtcctgagc agcaacatac tcttttgcta ccaggttcat cccgtcacgc agtggcgtca
    13921 ctaagcccac gtcagagtag cggaatattt tcatcagtaa tttacggtca aaatgctgat
    13981 tcaaataata aagcggcgtc cagcctaatt gcccgtattt accattaatt cgtccagctt
    14041 cattttcgag ctgatgacga atatcctgat aggcttgcac atcaccacgc gacgttg
//
LOCUS       DAJTNN010000102        14079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTNN010000102 DAJTNN010000000
VERSION     DAJTNN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.102"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1976
                     /gene="yicI"
                     /locus_tag="OO150_002685"
     CDS             <1..1976
                     /gene="yicI"
                     /locus_tag="OO150_002685"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5516775.1"
                     /translation="NLSARVSKGEFWSLDFLRNGERITGQDTNNQRNYMFERLDLGVG
                     ETVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQSVS
                     FEVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTT
                     SFTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIR
                     RLKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTN
                     PDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAHIYNELVW
                     NVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGF
                     WSHYIGGFENTAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQL
                     KCRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEA
                     GDVQFYLPEGRWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYA
                     WHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLR
                     NVVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(2029..3738)
                     /locus_tag="OO150_002686"
     CDS             complement(2029..3738)
                     /locus_tag="OO150_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5516776.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDAHLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(3859..5250)
                     /gene="xanP"
                     /locus_tag="OO150_002687"
     CDS             complement(3859..5250)
                     /gene="xanP"
                     /locus_tag="OO150_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5516777.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKTWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            5530..6735
                     /gene="gltS"
                     /locus_tag="OO150_002688"
     CDS             5530..6735
                     /gene="gltS"
                     /locus_tag="OO150_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5516778.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(6775..8856)
                     /gene="recG"
                     /locus_tag="OO150_002689"
     CDS             complement(6775..8856)
                     /gene="recG"
                     /locus_tag="OO150_002689"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5516779.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(8862..9551)
                     /gene="trmH"
                     /locus_tag="OO150_002690"
     CDS             complement(8862..9551)
                     /gene="trmH"
                     /locus_tag="OO150_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5516780.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(9558..11666)
                     /gene="spoT"
                     /locus_tag="OO150_002691"
     CDS             complement(9558..11666)
                     /gene="spoT"
                     /locus_tag="OO150_002691"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5516781.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(11685..11960)
                     /gene="rpoZ"
                     /locus_tag="OO150_002692"
     CDS             complement(11685..11960)
                     /gene="rpoZ"
                     /locus_tag="OO150_002692"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5516782.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(12015..12638)
                     /gene="gmk"
                     /locus_tag="OO150_002693"
     CDS             complement(12015..12638)
                     /gene="gmk"
                     /locus_tag="OO150_002693"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5516783.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     DEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            12896..>14079
                     /gene="ligB"
                     /locus_tag="OO150_002694"
     CDS             12896..>14079
                     /gene="ligB"
                     /locus_tag="OO150_002694"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5516784.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRQQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDEMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     SVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PE"
BASE COUNT         3155 a         3652 c         3885 g         3387 t
ORIGIN      
        1 gcaacttaag cgcgcgtgtc agcaaaggtg agttctggtc actcgatttt ctgcgcaacg
       61 gtgaacgtat taccggtcag gacacgaata atcaacgaaa ttatatgttt gagcggcttg
      121 atcttggcgt tggcgaaaca gtttatggtc tgggagagcg ttttactgcc ctggtgcgca
      181 atggtcagac ggtagagacc tggaaccggg acggcggcac aagtactgaa caggcgtata
      241 aaaatatccc gttctatatg actaaccgtg gctatggggt gctggtcaat cacccccaga
      301 gtgtctcttt tgaagtggga tcggagaaag tctccaaagt gcagttcagc gttgagagcg
      361 aatatctcga atactttgtt atcgacggtc cgacgccgaa agcggtactt gatcgttata
      421 cccgttttac tggtcgtccg gcgctgccgc ccgcatggtc cttcggcctg tggctaacca
      481 cttcatttac caccaactac gacgaagcga cggtaaacag ctttatcgat ggtatggcgg
      541 aacgcaatct gccgctgcat gttttccact ttgactgttt ctggatgaaa gccttccagt
      601 ggtgcgattt tgagtgggac ccgctgactt tcccggaccc ggaagggatg atccgccgcc
      661 tgaaagcgaa agggctgaaa atctgcgtct ggattaaccc ctatatcggt caaaaatccc
      721 ccgtctttaa agagttacaa gagaaaggct atttactcaa acgcccggac ggttcgctat
      781 ggcagtggga taaatggcag ccaggtctgg cgatttatga ctttaccaat ccggatgcct
      841 gcaaatggta cgccgacaaa ctgaaaggtc tggtcgcgat gggcgttgat tgctttaaga
      901 ccgactttgg cgaacgtatc ccaactgatg ttcagtggtt tgacggttcc gatccgcaga
      961 aaatgcataa ccattatgcg cacatctaca acgaactggt gtggaacgtg ctcaaggaca
     1021 ccgttggtga ggaagaagct gtcttgtttg cccgctcggc ctccgtcggt gcgcagaaat
     1081 tcccggtaca ctggggtggc gattgttacg ctaactacga atcaatggcg gaaagcctgc
     1141 gcggtggttt gtctattggc ctttcaggtt ttggcttctg gagccactat atcggcggct
     1201 ttgaaaatac cgctccggcg cacgtttaca aatgctggtg cgcgtttggt ttgctctcca
     1261 gccatagccg tttacacggt agcaaatctt atcgtgtgcc gtgggcctac gatgatgagt
     1321 cctgtgatgt ggtgcgcttc ttcacgcaac tgaaatgccg catgatgccg tatctgtatc
     1381 gtgaagctgc gcgtgcaaac gcgcggggta cgccgatgat gcgggccatg atgatggagt
     1441 tcccggacga tccggcttgt gattaccttg accgtcaata catgttaggc gacaacgtga
     1501 tggttgcgcc ggtgttcact gaagcgggcg atgtgcagtt ctacctgccg gaaggtcgct
     1561 ggacacacct gtggcacaac gatgagctcg acggtcgttg ctggcataaa cagcagcaca
     1621 gcttcctgag tctgcccatt tatgtgcgtg ataacaccct actggcgctg ggcaacaacg
     1681 atcaacgtcc cgattacgcg tggcacgaag gcacggcatt ccacctcttt aatctgcaag
     1741 acgggcatga agccgtctgt gaagtgcccg ctgctgacgg atcggtgatc tttactttaa
     1801 aagcagcacg tactggcaac acgattactg tgactggtgc gggcgaggcg aagaactgga
     1861 cactgtgcct gcgcaatgtt gtgaaagtaa atggtctgca agacggttcg caggctgaaa
     1921 gtgagcaggg gctggtggtg aagcctcaag ggaatgcgct gacaattacg ttgtaatcta
     1981 ttgatggaat gccgggtacg tctgagtcgt acccggcgag attgatgttt actgcaccag
     2041 ctctgccgac gtaacttcac cgctggaaac gatgccagca ttcatggttt gagtcacttg
     2101 ctgctttgcg gcgttcacgg catgcaattg cccgctaacc gtaggtttca gcggggcatt
     2161 ggcctgaata tctccactgg cggtaagctg aatattgccg tcgccagtca acggtaattc
     2221 aggccatccc cattgttgca aaatattcac cggcacaccg cgtccattca ggctgatatg
     2281 ggtctgacgt tgtggcgttt gtgaaacact ggcagtggct tccagaatgc ctttttcagt
     2341 aaatgcactc agttcgctga tattcaccgt gctgctgttg gcggtcagcg ccagcgacgg
     2401 gcgacgaaca tcaacacgat tgaatgtcgc ggcggcggca ttcagattcg ccgagccact
     2461 ccagacgccc catttatgat cggtaaccag cgtcaggttg gcaccgtaac catcgagcgt
     2521 ggtgagctgc cacgggaagt cagggtcgat atcaatgatc agattgcggc tggcgctaaa
     2581 tctcttcagt tgcaggctgt ttaaccaacc gggtgtcgtt tccatccaca actgttgcca
     2641 gtttttcggc aaggtatatt ccagcccggc aattgccgca tcatcaagga tcaacgtttt
     2701 cccgtcacgc agccagttcc ctgacgttct gaccataccc ccttcccagc ggctggtgaa
     2761 ctggcgcagc gctacgccct gcggggaaaa ttccgcgttt ataatcgggt caaataaatg
     2821 cagcgaacca taaatgaact cgctagcgtt catcgacagt ttgccttctt gtgtctgcca
     2881 gtcatcttta ctgaaggtca tgttgcgcaa gctgagatcg aggtcggtca ccgcccagtc
     2941 cggaccttgc aaatgagcat cgatcacttc caggcgacca atctgcaacg acgggacaga
     3001 gcgtaatggc gcaaagaagt cggttagcga tttttcgctt tgtagacgta tatccgccat
     3061 gcgcaggttt tccacttgcc agctgccgtc ggcgttacgc tgcgcgtttc cggttaatgt
     3121 cccgcgggcg atgtcggcac ccaggttagt cagcgtaacg cgatcgttat caatactgcc
     3181 ttcaatcagt acattggtgg caggaacatc attgagcgaa agcgatccgg cactaaactg
     3241 aatctgcgcc ttcgtaccca gcactttacc ggcttttggt gaccacggaa ctacgccgcc
     3301 atttacccgc tgcgcgctca gtttccattc gctattcggg ctattaaacg ccatatcacg
     3361 cagttgcaga cgatcggctt tgaacggtag cggcgcggtc tggtcggtga gattcagcgt
     3421 cccgttttcc agcaggatgg tatcgacatg gcgtggttcg gtcagttgcc gactgcttag
     3481 cgcaatgtcg acacttttgg ccaccagggt cgcgggctga ccatcacgac caaacgtgac
     3541 attctccagc acgatatgag atggcgcgga aaaacggtga tccatcgccc cgaaggccag
     3601 atgatagtcg ctattctcgg aaacccatgc gctgatatgt tctgctcccc agcgggtttg
     3661 cagaagaaaa tagaggccag cgatcaccac taacagagcg atgagaatgt agagaagcag
     3721 cttcccaata aatttcatgg tcttccattc cgtgaaatgc acataagaga gttatgcacg
     3781 atttacgcgc aatcctcaag gcgggaatgg tgaaagaggt gagactgcgg cggtaattat
     3841 cttaccgccg ccagtgaatt actgtttttc tggtgggaaa atcagattca gcacgatggc
     3901 agtaataccg cccgcggcga tcccggagga gagcaggttt ttcagccatt caggggcaaa
     3961 ctgcaaaatc agcggctgct gagacacgcc cagaccaacc gccagcgaca gcgcgataat
     4021 cagaatcgcc cgacggttca gcggctcacg agaaacgata cgcacaccgg aggcggcgat
     4081 ggtgccaaac attacaagcg ttgcgccgcc cagaaccggt tctggaatgt gttgtacaaa
     4141 accgctcact gccgggaaca gacccagcac gatcaacatc agcgcgacga caaaaccgac
     4201 atagcggctg gcaacaccag tcaactggat cactccgttg ttttgcccga agcaggagtt
     4261 cgggaaggtg ttaaacaccg ccgaaacaaa cgagttcagg ccgtttgcca gcacgccgcc
     4321 tttcaggcgt ttcatgtaca gcggaccgga cactggctgt tcggaaacgt cagaggtcga
     4381 cgtgatatcg ccaatggttt ccagcgaagt gatcataaag accagcatca gcggcagcag
     4441 cagactccat tcaatgccaa gaccgtaata gagcggcgtt ggcaccataa tcagttcttg
     4501 cgtcatcggt tcgttgcttt ctggcaacat gcccataaac cacgccagcg catatccggc
     4561 cgccatcgca atcaccagtg aggccacgcg taagtaaggg ttacgttgac ggttaagcag
     4621 gataattaag gctaagacca cgcctgccag cagcagattt ttcggtgcgc cgaaggtgtt
     4681 atcgctcatg gctgcgtaac cgccgccaat ggacgttaac ccaacctgaa ttagcgacag
     4741 gccgataatc atcaccacaa cgccagaaac cagcggcgta ataatgcggc gcgccagatg
     4801 cagaacgcgg gagatcacca tctcggtgca acttgccagc atcaacgtgc cgaacaaagc
     4861 cgccatcatg gtaggaacat cagcaccacc ggttttcagc gcggtaccgc ccataatcag
     4921 cggggcaaca aagttgaagc tggtgccctg aatagacaac agcccggagc caaccggacc
     4981 ccaggtctta atttgaataa tcgatgccac accggaggca aacagcgaca tactaataat
     5041 gtgttgcgtg tcttgtgccg gtaaacccag cgcctggcag attaatagcg ctggcgtgat
     5101 caccgcaacg aacatcgcca gcagatgctg acaggcggca aacagggttt gaggaagcgg
     5161 cggacgatct tcaagacggt aaatcagttc gctgttttga gtctgcgcaa ccggttgcgc
     5221 attttctgac tcgagggtgg aaacagacat cgaaaacaat cccgtggtag caaagcgggc
     5281 attttagctg accgaaagag aaaagcaaac gtttgccaca tcctcatcaa aatatttaaa
     5341 tgagataaat gcactttttt atataacttt tggtattttt ctgcttaaaa tccatgccat
     5401 ttgttcatta acagctgaga atttgaccca ggaaagcata actcgatacc cgtcatagtt
     5461 catgaagttg ccactgcgcg atgaagtatg acgagtatga aagagtgatg cggatacaaa
     5521 ggagtaacta tgtttcatct cgatacttta gcaacgcttg ttgccgcaac gctgacgttg
     5581 ctgctcgggc gtaagttggt ccattccgtc tcctttttga agaaatacac cataccggaa
     5641 cctgttgcgg gtggtttgtt ggtggcgctg gcgctactag tactgaaaaa aagcatgggc
     5701 tgggaagtca actttgatat gtccctgcgc gatccgttaa tgctggcttt cttcgccacc
     5761 attggcctga acgccaacat tgccagtttg cgtgccggtg ggcgtgtggt tggcatcttc
     5821 ttgattgtgg ttgttggtct gttggtgatg caaaatgcca ttggcattgg tatggctagc
     5881 ctgttagggc ttgatccgct gatggggctg ttggccggtt ctattacgct ttccggcggt
     5941 cacggtacgg gcgctgcgtg gagtaaattg ttcattgaac gttatggctt caccaatgcg
     6001 acggaagtgg cgatggcctg tgcaacgttc ggtctggtgc tgggcggctt gattggcggt
     6061 ccggtggcgc gctatctggt gaaacactcc accacgccga acggtattcc ggatgaccag
     6121 gaagtcccga cggcgtttga aaagccggat gtgggacgca tgatcacctc gttggtgctg
     6181 attgaaacta tcgcgctgat tgctatctgc ctgacggtgg ggaaaattgt tgcgcaactt
     6241 ttggctggca ctgcttttga actgccgacc ttcgtctgtg tactgtttgt tggcgtgatt
     6301 ctgagcaacg gtctgtcaat aatgggcttt taccgcgtct ttgagcgtgc ggtatccgtg
     6361 ctgggtaacg taagcttgtc gttgttcctg gcgatggcgt tgatggggct gaaactgtgg
     6421 gagctggctt cgctggcgct gccgatgctg gcgattctgg tggtacagac catcttcatg
     6481 gcgttgtatg ccatcttcgt tacttggcgc atgatgggca aaaactacga tgcggcagtg
     6541 ctggctgcgg gtcactgtgg ttttggcctc ggtgcaacgc caacggcaat cgccaacatg
     6601 caggcgatca ctgaacgctt tggcccgtcg cacatggcgt ttttggtggt gccgatggtc
     6661 ggtgcgttct ttatcgatat cgtcaatgcg ctggtaatta agttgtattt gatgttgccg
     6721 atttttgccg gttaaccgat gaagcggaag cggcggtctt ctcaccgccg ccttttacgc
     6781 attcgagtaa cgttctgtct ccggcatcca gcgttctatc aaggcttttg cctgttgtgg
     6841 gtaacgttcg tgaatatgtc gcgccaggcg ctgaacttcc gggatcatcg cctgatcgcg
     6901 cagtaaatcc gccactttaa attcagcatt acccgtctga cgcgtgccta acaattcgcc
     6961 agggccgcga atctccagat ctttttgcgc aatcacaaaa ccgtcgttac tgtcgcgcag
     7021 cacttgcagg cgaatttgcg ccgttttaga aagcggcgtt ttgtagagca gcacgcagtg
     7081 agaagccacc gcgccacgac ctacgcgccc gcgcagctgg tgtaactgcg ccagacccag
     7141 acgctcaggg ttttcgataa tcatcaggct ggcgttaggc acatcaacac cgacttcaat
     7201 aacggtagtg gcaaccagca gatgtagctc accttgttta aacgacgcca tcaccgcctg
     7261 tttctcggct ggtttcatcc gcccgtgtac caggccaaca ttcaattctg gtagcgccag
     7321 tttcaactct tcccaggtag cttccgccgc ctgcgcttcc agtaactctg actcttcaat
     7381 caacgtacaa acccagtacg cctgacgacc ttcagttatg caggcatggc gcacgcggtc
     7441 aatgatgtcg gtacggcggg tatcaggaat agcgaccgta gtcactggcg tgcggcctgg
     7501 cggcagctca tctatcaccg aggtatcgag atcggcatac gccgtcattg ccagcgtgcg
     7561 ggggatcggc gtggcggtca tgatcaactg atgcggatgg aagccttgct gctggccttt
     7621 ctcccataac gccagacgct gatgcacgcc aaagcgatgt tgttcgtcga taatcaccag
     7681 cgccagaccg ttaaactgca cctgttcctg gaagatggcg tgcgtaccaa caatcatctg
     7741 cacctgaccg ctggcgatgg cttcctgctg agacagtcgc gctttgcctt tctgcttacc
     7801 ggcgagccag cccacttcga taccgagcgg ttcaaaccag ttgcggaagt tattggcgtg
     7861 ctgttcggcg agtaattcgg ttggtgccat caatgccacc tgtttgccgt gggcaatcgc
     7921 acgcagcgcg gcgagggcgg cgaccagcgt tttaccggaa cctacatcgc cctgtaccag
     7981 gcgcatcatc ggcacatcca gcgccatatc gcgctcgatc tccgccacta cgcgtgcctg
     8041 tgcgcccgtt ggcttgaacg gtaaggctgc gaggagttta tttttcagcg catcattggc
     8101 gctcagcggc tgggcatgaa aacgctgtgc tccggcacgt aaggctaaca tgctgaggtt
     8161 gtgcgccagc agttcttcca gaatcagacg acgttgcgcc ggatgctgcc cggtttccag
     8221 atcgctaagc tgtagcgtcg gtggcgggcg gtgcaaagtg cgcaacgctt ccggtagcgt
     8281 catcattcct tgtgacagtt ccggcggcag gagttcttca atggcgcagg tgtcgagcag
     8341 atccagcgcc tggtcggtta atttacgcag cgtggcctgc tttacgcctt ccgttgttgg
     8401 ataaaccggc gtgagcgttt cctgtaattc tggcgtgctg agatcgccct gcacgcggta
     8461 ttccgggtgg atcatctccg caccatattt accgcgcttt gcttcgccat aagccagtac
     8521 acggcggccc gtcgccaggc tatttttcat tgccgcgctg aaattgaaaa agcgcatggt
     8581 gaggatgccg gaaccgtcgc tgatctggca ggtcatcatc cgccgaccgc cgaaggagat
     8641 attgcagttc agcacttcgc cttccaccgt ggcataaacg cccggcagta gttctccgat
     8701 ggggtagaga tgggtgcgat cttcgtagcg caggggaagg tgtaagagta gatcctgcac
     8761 ggtatgcagg ttgattttcg ccagtttgtt actaagtgct gcgccaacgc ccgttaggga
     8821 actgagtggg acagcatcta acaggcgacc tgtcatggca cttaccctgc agcctgcata
     8881 gtagcccacc agtcggcatc agcttcgatc tcgccttgct gattgacgtg gggataaggc
     8941 aggcctttgc gttttgcgac tttcgccagc accggatagc cgccttcaaa caacaggcgt
     9001 tgttgctctg cttccggcaa catgctgttt tcacgcaggt acatgcctgc attttgccgc
     9061 tgacgctggg cttcgtaaag aatgagggct gaggcaacgg aaacattcag cgactgcacc
     9121 atgccgatca tcggaatgat gatgtcctga tccgccaggg ccaatgcttc ctgcgtgatg
     9181 cccgttttct cctgtcccat caaaatgcag gtcgggcgag tgtaatcaat ttcgcggaaa
     9241 tcgacagcgt tatcagaaag atgggttgcc agaatctgca tgccctggcc tttgagatga
     9301 gcgacggcat cgccaatggt gcggtgtgtt ttcacctgta cccagctgtt actacccgcc
     9361 gctgccgaag ccatggtgcg catgcggcta ccaggccaga cggcgtgaac ttcatgtacg
     9421 ccaacggcat ctgcggtacg aataatcgca gaaacgttat gaggtttgtg gacctgctcc
     9481 atgcagacgg tcagatcagg ctgccgcctg gcgagcattt cgcagatgcg tgcataacgt
     9541 gttgggttca taaaacatta atttcggttt cgggtgactt taatcacgtc tggcatcacg
     9601 cggattttgc gcatgatatt cgccagatgc acacggtcac gagcggtcag acgaataaag
     9661 gcgctgtaga cgcgaccatc tttctcttcc gtattcaaac tttgaatatt cgaagtcgtg
     9721 gtgttaattg ccgccgtcag gtttgccagc gcaccctgat gattgaacat ctccaccttg
     9781 atttcggtga tgaactcctg cgccgtctct ttatcccatt ccacagccat aaacttctct
     9841 ggctctttct ggtagccacg gatgttacgg caggattcat ggtggatcac cagaccttta
     9901 ccggggctga cgtgggcgat aatcgggtcg ccaggaatag ggcggcagca tttcgcaaag
     9961 gtgatcagca cgccatccgc gcctttgatc ggcagatgcc cgtggctttg agtcgccggt
    10021 ggaatggagg cgtccccatg ttgtagattt ttcgcgacca ccacgctcat tgcgttacca
    10081 aggccgattt ctgccagcag atcgtcaagc gttgccagct tcatgcgatc cagctcgcgc
    10141 tgaatatttt cctgcgggat ttcattgagc ttacggctac cacccaaagc atggttgagc
    10201 agacgacggc ccaggcttac agaatcatca cgcttgaggt ttttcagcaa ctgacgaatt
    10261 ttggcgcgcg ctttcgagct aacgacaaag ttcagccaag cggcattcgg gcgagcgccc
    10321 ggagcggtaa tgatttcaac ggtctgaccg ctagtaagcg gctgcgacag cgggtaaggc
    10381 tggcggtcga cgcgtgcgcc cacgcaggca tggccgatat cggtatgcac tgcataagcg
    10441 aagtcgacgg gcgttgcacc ggcaggcagc tcgacaatgc gcccttccgg tgtgaaaacg
    10501 taaatctcat ccgggaagag atcggattta acgctctcga taaattcaaa cgaactaccg
    10561 gcgctctgtt gcagctccag caggctttgc atccagcgct gggcgcgtat ttgtgcggta
    10621 gtactggttt cgccgtgctc tttataagcc cagtgcgcgg caacacccat ctccgccatc
    10681 tgatccatat cttcggtacg gatctggacc tcaaccggca cgccgtgcgg gccaatcatc
    10741 gaggtgtgca acgactgata gccgttcgct tttggaatgg cgatatagtc tttcacgcgg
    10801 cccggacgcg gcttgtacag gctgtgcatc tggcccagca cgcgataaca ggtgtcagaa
    10861 tcattgacga tcacgcggaa agcgtagatg tccatgattg agtgaaaacg ctgctctttg
    10921 agcaccattt tgcagtaaat cgaataaaga tgcttttcgc gaccactgac gcggcacggt
    10981 attcccgctt cctgcaaacg cccttcgatt tcagaaagaa tcttctggat catctcttta
    11041 cggttgccgc gcgcggcttt caccacttct ttgattacgc gataacggtt gggatacagc
    11101 gcctcaaaac ccagctcttc gagttcggtt ttaatgtggt ggatacctaa acggtgcgcc
    11161 agcgggctgt aaatttcgag agtttcacgg gcgatgcggc gacgtttgtc cgggcgaagt
    11221 gagcccagcg tgcgcatgtt gtgggtacgg tcggcaagtt tgatgaggat gacgcggata
    11281 tcctgcacca tcgccataat catcttgcga aagttttcgg cctgcgcctc tttcttatcg
    11341 cggaacttga gtttatcaag tttcgacacc ccctctacca gctcggcgac gcttttacca
    11401 aaaagctgtt ccatatcctg gtaggtggcg ggagtatctt caatcacgtc atgcagcagc
    11461 gccgccatca gcgtttcata gtcgagtttc atctcggcca gaatgcaggc aaccgctacc
    11521 gggtgcgtga tatagggttc accgcttgaa cgtgtttgcc cctcgtgagc atcacgtgca
    11581 acgagatacg cctgccgcag acgcttgatt tggtcttccg gcaggtaggt ttgaatcagt
    11641 tgattcaggc tttcaaacag atacaagggc gacccgcttt gtgattaacg acgaccttca
    11701 gcaatagcgg taacggcttg taattcagcg gcttcctgct cttgctgttc ctggcgttcg
    11761 cgaacgtcga ggatctggtt gttgatcaga ccttcttcga tttcgcgcag cgcgattaca
    11821 gtggttttat cgttttcttc cggtaccagc ggatcctttc cgcctacctg catctgacga
    11881 gcgcgacgcg cggcgaccag taccaggtca aaacggttac caattttctc tacagcgtcc
    11941 tgaacagtta cgcgtgccat acttaaaaag ctccacaggt gaagaaatga ctgggcatga
    12001 tactgaaatc aggttcagtc tgccaacaat ttgctgatta aagcgtcatg acgctgcttt
    12061 tggcggctca tgcgcagacg ttcggcgcga ataatggtct tcaaatcggt caacgcggta
    12121 tcgaagtcat cattcacaat caggtaatca tattcgtcgt aatggctcat ttctgcaaca
    12181 gcttgcgcca tacgctttgc aatgacctct tcgctgtcct gaccgcgacc gcgtagacgg
    12241 cggtccagtt caattttaga cggcggtaaa ataaagatac tccgcgcgtg cggcatcttc
    12301 tggcgaattt gctgcgcgcc ctgccagtcg atatcgagaa aaacatcgac accggtcgcc
    12361 agtacttgct caatggcctc acgcgaagtg ccatagtaat taccaaaaac ttctgcgtgt
    12421 tcgaggaacg catctctgct aatcatttct ttaaattcat catgattaac aaagaaataa
    12481 tgttcaccgt ggacttcacc aggacgcggt tggcgtgtgg tgtgtgaaac agaaacctgg
    12541 gtgtcataca acggttgggt ttttaataaa gcctgaatca ggctggattt acccgcgcca
    12601 ctgggggcag aaacaatata aagcgtgcct tgagccatga gtatctttcg tatgtgatta
    12661 gcgaaataaa gcctacatac gagcttatta tacacggcgc ggccatgtga cgtagccctt
    12721 gctcacactt tttgcgcaaa gttgttgcgt tttgcgtagc tgcttccgca tttgcctgtg
    12781 atttactgca ataaagacga ttctcaggaa gcgtcctcgc agactccgtt tttgccactc
    12841 ggctttgcct tcaccacgtt gtatatctgc cagcctgact acagcggagg tgaagatgaa
    12901 agtatggatg gcgatattaa taagtatctt gtgctggcaa tcatctgtgt gggcggtctg
    12961 tccggcctgg tcgccagcca gagcacagga agaaattttc cgccagcaac agcaaataaa
    13021 acagtgggac gatgactact ggaaggaagg aaaaagtgag gtggaagacg gtgtttacga
    13081 tcagttaagc gcccgtctta cgcagtggca acgctgcttt gtgagcgagc cccgcgacga
    13141 gatgatgccg cctttaaatg gtgcggttat gcatccggtt gcccatacgg gtgtacgcaa
    13201 aatggcggat aaaaacgcat taagtctgtg gatgcgagaa cgtagcgatc tttgggtgca
    13261 gccaaaagtt gatggcgttg cggtaaccct ggtttatcgg gacgggaaac tgaacaaagc
    13321 aatcagtcgc ggtaatggcc tgaaaggcga ggactggacg cagaaagtta gcttaatttc
    13381 cgctgtgccg caaaccgtta gcgggccttt agccaacagt acgcttcagg gggaaatatt
    13441 tctccagcgc gaggggcata tccaacaaca aatgggggga ataaatgccc gcgcaaaagt
    13501 tgctggcttg atgatgcgcc aggacgatag cgacacgctg aattctctgg gcgtttttgt
    13561 ctgggcatgg ccggatggac cgcagttaat gtctgatcgt ttaaaagagc tggctaccgc
    13621 aggttttact ctaacgcaga cgtatacccg tgcggtgaaa aatgctgatg aagttgcgcg
    13681 cgtacgcaat gagtggtgga aagcggaatt acccttcgtc accgatggcg tagttgtacg
    13741 agcggcgaaa gagccagaat cccgccattg gctaccgggc caggcagagt ggctggtggc
    13801 ctggaaatat caacctgtag ctcaggttgc cgaagtgaag gcgattcagt ttgcggtggg
    13861 taagagcggt aaaatatcgg tggttgcgtc actcgcatct gtcatgctgg atgataaaaa
    13921 agtccagcgg gtgaatattg gttccgtcag gcgctggcag gagtgggata ttgcgcctgg
    13981 tgatcagatt ctcgtcagcc ttgccggtca gggtattcct cgcattgatg atgtggtgtg
    14041 gcgcggtgca gaacgtacaa aaccgacacc accagaaaa
//
LOCUS       DAJTNN010000103        13720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTNN010000103 DAJTNN010000000
VERSION     DAJTNN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.103"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(89..775)
                     /locus_tag="OO150_002695"
     CDS             complement(89..775)
                     /locus_tag="OO150_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5516785.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OO150_002696"
     CDS             complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OO150_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5516786.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OO150_002697"
     CDS             complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OO150_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5516787.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OO150_002698"
     CDS             complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OO150_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5516788.1"
                     /translation="MTINFCRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITAQAEDLAQLRGVLGLD"
     gene            complement(5228..6056)
                     /locus_tag="OO150_002699"
                     /pseudo
     CDS             complement(5228..6056)
                     /locus_tag="OO150_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            6092..6394
                     /locus_tag="OO150_002700"
     CDS             6092..6394
                     /locus_tag="OO150_002700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516789.1"
                     /translation="MQRKVNRSDSDYHYGKEKYKRNVYKMMLECKQREVMPGDINDYS
                     RVERWDCLQRTMCSCRMPRKRHIQPTKLSRLNASELPCKTDKRVYQAISSIRDYQI"
     gene            complement(6725..8233)
                     /gene="putP"
                     /locus_tag="OO150_002701"
     CDS             complement(6725..8233)
                     /gene="putP"
                     /locus_tag="OO150_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR5516790.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(8392..8601)
                     /locus_tag="OO150_002702"
     CDS             complement(8392..8601)
                     /locus_tag="OO150_002702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516791.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            8656..12618
                     /gene="putA"
                     /locus_tag="OO150_002703"
     CDS             8656..12618
                     /gene="putA"
                     /locus_tag="OO150_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5516792.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNCNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTYETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12658..13296)
                     /gene="rutR"
                     /locus_tag="OO150_002704"
     CDS             complement(12658..13296)
                     /gene="rutR"
                     /locus_tag="OO150_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5516793.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            13584..>13720
                     /locus_tag="OO150_002705"
     CDS             13584..>13720
                     /locus_tag="OO150_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5516794.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     AL"
BASE COUNT         3404 a         3733 c         3379 g         3204 t
ORIGIN      
        1 tttaaagaag agctcgtacg aaggtacggg ctttttttcg tatattgcac acaccggggg
       61 gatgagaagc cccgaccggg atacttagtt aatgcaccgg ttcactcttt gtcgccgcga
      121 ctaatgggcg aattttgtca cgagtctccc agaccggagg aatttcctcc aatttagtga
      181 cagaagctcg tacaaaaggc acccgtgcaa gagtcataat tgtatccaac caacggtcac
      241 gaataatgcg atccgccctt tcatgactcg ggtcatccag ctcaataaca cagactattt
      301 tattgttctt ctttgaaacc agaacataat ccattgccat gccattataa attgcaaaga
      361 aacgctttct ttcttcttcg ctaaactcag gttgttctgg aaagttgatc aagcgcccca
      421 gagagacctg gcaatggata tgacaataat gtccaaacca tttttccaga ttatcgaaat
      481 acattttttc acaacgagtg attaaccact cttttctttc caataaaata ccttcggcaa
      541 ccattccctg aaataactca tctcgctctt gccaacgaga gtcacgatag gttttctgcg
      601 gtatatagct ccttgtcgac gccttagcgg caggggaagt tgcagatgaa gggacgggtt
      661 gcgatttagc gatgattacc gatgaggcaa cttttcggag tgtcaatttt ctggcaacag
      721 caaaaccggc gataaatgca attgtcaaac aagttacaaa agccataaag ctcataatta
      781 attttcacct atccatgttg attatatttc attaccaatg ttactcgctg cagcgtgtta
      841 catgccaaca atgcaacgat caatatatag ttagtatcat gttttgatta ttacaaccat
      901 ccctatttag ccattgcggc ttgccttcca caaaaaaata acaacacaca atattagcaa
      961 agaattaatt aagattaacc ttacatttat aatattatcc ctaaagaata aatcagagga
     1021 atatattcgg tgttaaacag tcagtcaaag tatagcccat tgttgattaa acctcataag
     1081 caaaattggc accagatctc ctgtacaggt tgaatggccg aaaaaagata ggcagatgct
     1141 catttttttg gcttctggaa gaaaaactct ccgcgctggc agtattacac tggtctgatg
     1201 ctatgacgaa aaatgcctgg taagagtatc taaaccagac tccagttcag cttatcaaca
     1261 cgctcaaagt cgcccccctg aacgctaacc tgaagtgaaa atctgttgaa ataaaacaaa
     1321 gcccggttcg cccgggctct gcaccgataa cacacttaac tgtatgcatg cagcgtacgt
     1381 tggcaaagtg ccgaacgtac gcaatcctct tttccgaacc ggacgatccc aaccatttca
     1441 tcttcttcga aacgttccag cgcgtcactt aatccggagc acacgccgcg aggcaaatcg
     1501 cattgcgtga tatcaccgtt gacgataacc gtcacgttct ccccgaggcg ggttaaaaac
     1561 attttcattt gcgcggcagt cacattctgc gcctcgtcaa gaatgacgac tgcattttca
     1621 aaggtacgtc cacgcatata ggcgaacggc gcgatttcca ccttccctat ttccggtcgc
     1681 aggcagtact gcataaagga agcccctaag cgccggacca gcacgtcgta gaccgggcga
     1741 aaatagggag caaacttttc tgcgatatca ccaggtaaga agccaagatc ttcatcggct
     1801 tgcagaactg gacgggtgac gataatcctg tcgacatcct tatgtatcag ggcctctgcc
     1861 gcttttgctg cgctgatcca ggtttttccg cacccggctt cgcccgtggc gaatatcagc
     1921 tgcttactct caatagcctt cagatagtgc aattgcgctt catttcgcgc gaggatgggc
     1981 gaggtatcgc gactgtcgcg ggccatacca atggcttcta cgccgctcat ctgcacaagc
     2041 gaggtgaccg attcttcttc acgctgctta tggctgcgcg aatcccgtct cagcacacgt
     2101 tttgcctcgc gacgagcttt gatcactgct ttttgtcttc ccatggagag caccttgagt
     2161 tgtttgtatt catcacacgc gccgttggca gcgcgattat gcgcacgaac atcagagggt
     2221 tggcttcctt gtaagccata gtttgctttt ggataaaatg ccgaaaaacg gctacgcgca
     2281 ccgtttacgg cgtcggtaac acatgaaaag aaaggatgag gttgaaaatg caaagtgacg
     2341 agatgactac cggaggagaa aactccgcga gtggtggcgc gttgattatc taaaacatgt
     2401 ccagtacagg acgttaccat ccgcgatctc catagtgact gactatcact gccgggaact
     2461 tccgctgcta cttaataagt acaacagatc tcgcatttta ttgcaacaat atatttactt
     2521 atatttaact ataaaacact atttcagtga cattagtttc tactggaaag atgacagagt
     2581 gatgacagtg atgaaaaaag ctgtgtgctt tcagcaggat ttgcatcagg catcagtgca
     2641 cgatcgccgg atgcggcgtg aacgccttat ccagcctacc aaacccgcac tgattgtagg
     2701 catgataaga cgcgcaagcg tcgcatcagg catctgcgca caaatgcctt atccgcctaa
     2761 aaacattaaa cccgcaataa cgcgcttccg agataatcgt tcgcgtcctt cactcccggc
     2821 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     2881 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     2941 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3001 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3061 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3121 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3181 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3241 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3301 ttatcattct ggctatcggg attggcagtg ccgtctttaa agcccagcaa attaatcggc
     3361 gtctctttgc ctttactacg cgctgcgtga tcggaaataa acccttcccg cttccagcgc
     3421 acgctgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggat caccgtgtcc
     3481 tgggtgttgg cgcaaatttg tagcaacaca tcaccatggc ataacgccgc atccagcgag
     3541 tcgttgggga aacgcgtcat cttctgcaac tttttcggca tctgtggcgc aaggccaaac
     3601 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     3661 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgcc
     3721 gctccgccct gagtcagaaa agcaaaacgc tgagtcaaca agcgaaacaa ccgttcaaga
     3781 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     3841 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     3901 gaaagcgtac ccggcgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     3961 agcgccagcg cacctatcac tttcagtaaa cgtcggcgtg acggttcatt cacgccgttt
     4021 tcatctttat actgcataac gcttaatcca gtcccagcac accgcgaagt tgcgccagat
     4081 cttccgcctg cgcagtaatc ggtcctttca gtgcattccg gtcggcatcg gtcaatttgt
     4141 cgtaggtttc aaaaccgtct ttagtacggt atttcgccag aatggtatcg acctttttaa
     4201 agttggcatc gacttttgcc agcagttccg ggttggcttt ttgcagttgt ggacgcagca
     4261 aatcgacaat tttctgcgag ccttcaacgt tagcctggaa atcccacaga tcggtgtggc
     4321 tgtagcgatc ttcttcaccg ctaattttgc tggctgccac ttcctcaatc agtccggctg
     4381 cgccgccgac cacttttgaa ggtgggaaag ccagttcact gatgcgtttt tgcaaatcga
     4441 ccacatctgt ataaagctgc tcagcgtact gatccatccc tttggtggtg ttgtcgccaa
     4501 acaatgcttt ttccagacgg tggaaaccag tgaattttgg gtcggcggct ttttgctcgt
     4561 aatcatcttc acgggcgtca atgctgccat ccagatcgga gaacagttca gcaatcggtt
     4621 caatgcgctc atagtgctgg cgcgtcggcg catacagtgc tttcgctttt tcgatatcgc
     4681 cagctttaat cgcgtcggta aaggctttgg tgtcggtcac cagctgcgtg gtttccgcca
     4741 tgacatacgc tttatatgca gtaattgcac caccaagact taacagcgca tcactttgcg
     4801 ccgcatccgc cgttgcctca cctttgacga tcaacttccc tttcgggtta gtcagcagac
     4861 cgcaggtcat atcgtattcg ccaggctgta aattcgccgt cattttctgg ctaaaaccag
     4921 gggcgatatt ttcccgctct tccaccacca tcacgccttt gaggatctcc cactccagcg
     4981 ccttctggct atggttctga ataatgaact gtgttttccc ggcgttaacc gtaatggtca
     5041 tcggttcgca ctgcttatcc gtcacggtca ctttgacctg cggcacatca gcggcgttag
     5101 ccataaaagc agaagaaaac agcgcagcca cgctcaactg caatgcgtta cggcagaagt
     5161 taatggtcat gacgagacta tccctttaaa gtatgttgta actaagcaag agtttgcgtc
     5221 gtatttgtta cgcggagcga gacgctgtcg cccctgcgcg tggtggcaga gcaaatgcca
     5281 ccagcgccgg gatgagataa ataaaccaga cggcgacttc gctgacgctc ggcgcttcct
     5341 gatagccaaa aatcccttcc atcagcgtgc caaacagcga gtgagttgag agcaccgcac
     5401 tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc ggcttcatga aatgcgcgaa
     5461 tggcaccagc tgccagccct gcggcgacga agagaataaa caggctggtc catttaaaaa
     5521 atgcaccaag attgaggcga ataccgcccc agtagagcag aaagccgagc accacggctg
     5581 tagcaagacc gagcattgca cccagcggcg gccagatccc gacatcttgt tgaaatgccg
     5641 ccagcaggaa aaagaccgac tccagccctt cccttgcaac ggcaaaaaag accatcatca
     5701 ccagcgccca gccatgatga tttccacgct acaatgcgct atcgactgcc tgttccagtt
     5761 gcacttacgt tgcgcgacac tttgcgcatc cagaaaacca tccaggtaag gatcaccacg
     5821 gcgatcaccg ccacgatacc ttcaaacagt tcctgttctt tttgcggaaa ttcgccggtg
     5881 gtttcgttaa tgaagatccc caggcccagg cacaacgcag cggcaagcaa cacgccaatc
     5941 cacatcacac caatccatcg gcctcgctgg gtacgcttaa gatagctggc aatcaaactg
     6001 acaatcagcg cggcttcaag tccttcgcgc aacataatga gaaacggaac aaacatgcca
     6061 cccaccctta aacgtttttc tgagtcaatc tatgcaaaga aaagtaaatc gcagtgatag
     6121 cgattatcat tacggtaaag aaaaatacaa gcggaatgtt tataaaatga tgttggaatg
     6181 taaacaaaga gaggtaatgc caggggatat aaacgattat tccagggtag aaagatggga
     6241 ttgtttgcaa agaaccatgt gttcttgccg gatgccgcgt aaacgccata tccagccgac
     6301 aaaactgtcc agactcaatg catcagagct tccatgtaaa actgataagc gggtgtatca
     6361 agcaatttcg tccatccgcg attatcaaat ctaacttttt gcccggaaca taataacagc
     6421 tcgggtgttg gtggcgtcaa aatcgctgaa gcgttccccg aaggccggga cagcccacat
     6481 ggatgtgggc tgagggcgcg tcttacagga cgttacctcg cgcccgaccc ggtagccgaa
     6541 ggggataagc tgaaggtacc gcgcagcggc gattttgttc gccagagccc gggggtgcac
     6601 agggggcggc ggcgattggc cgccccctgc gcgctccctg caccattcaa tttatatggc
     6661 gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc gcaggctaag
     6721 tcccttagct ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca tcggcctcgg
     6781 caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac actacaatcc
     6841 cgatactgcc gaagatgaag cccggaataa tttcgtacag tcccagccag ccgaactgct
     6901 tccagacgat aaccgtcagc gcaccgatga tcatccccgc cagtgcaccg ttacgcgtca
     6961 tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca aagcctgccc
     7021 acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc gcaatcgcca
     7081 ccagcgccac caccagcacc atcacacgcc ctacccacac cagctctttc tggctggcat
     7141 gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac accagcagtt
     7201 ggcaacttaa ggttgacatt accgccgcca gaattgccga cagcagaatc ccggcaatcc
     7261 acgggttaaa cagaatttgc gccagttcga taaacacgcg ctcggcgttc tggtttaccg
     7321 cgcctgctac cgccgggtgc tcattaaagt aagcaatccc aaagaagccg acagccaccg
     7381 cccctgccag gcagaggatc atccaggtca tactgatacg acgcgcatgg acaatgctgt
     7441 ggtgagaatc cgccgccata aaacgcgcca gaatgtgcgg ctgcccgaag taacccagcc
     7501 cccaacccat cagtgagata atggcaacaa agttcagacc tttgagcata tcaacgtttt
     7561 cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca ctgataatga
     7621 cgataaccgg tgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca gtgtcagtcc
     7681 agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgccgca gctcccgccc
     7741 acagagccgt ttcgtagctc atgccaaagg tactttcaaa cagacgcgcg cctgccacaa
     7801 tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag ataatgcgca
     7861 aaatgcggct tttatcttca aaacgcccgg tgaaataatc cggcaatgtt aaggcgttat
     7921 tattgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg atccacgcgc
     7981 ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa accgcgccag
     8041 gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat gccgtcacaa
     8101 atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt gatcgccagg
     8161 cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc aacatcggtg
     8221 tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa acttgccgtt
     8281 atatctgcaa ccggaacggg gtaacagagt ttatttttta ccagggcgac cgtatcctgc
     8341 cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat tttagtgctc
     8401 agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg cacctttcaa
     8461 aaatgttaac tgccgcagag aaaaagtctg agttattttt ttaatccctg tcatatcaat
     8521 ttcttttatt aacatttcat tcatttttaa gcttgctacg caggtcacat ttaacatggt
     8581 tgcacaaagt tgcaacatca tggatatttc acgataacgt taagttgcac ctttcagaac
     8641 aacaggagta atggcatggg aaccaccacc atgggggtta agctggacga cgcgacgcgt
     8701 gagcgtatta agtctgccgc gacacgtatc gatcgcacgc cacactggtt aattaagcag
     8761 gcgatttttt cttatctcga acaactggaa aacagcgata ctctgccaga gctacctgcg
     8821 ctgctttctg gtgctgccaa tgagagcgat gaagcaccga ctccggcaga ggaaccacac
     8881 cagccattcc tcgactttgc cgagcaaatt ttgccccagt cggtttcccg cgccgcgatc
     8941 accgcggcct atcgtcgccc ggaaaccgaa gcggtttcta tgctgctgga acaagcccgc
     9001 ctgccgcagc cagttgctga acaggcgcat aaactggcat atcaactggc cgataaactg
     9061 cgtaatcaaa aaaatgccag tggtcgcgca ggtatggtcc aagggttatt gcaggagttt
     9121 tcgctgtcat cgcaggaagg cgtggcgctg atgtgtctgg cggaagcgtt gttgcgtatt
     9181 cccgacaaag ccacccgcga cgcgttaatt cgcgacaaaa tcagcaactg taactggcag
     9241 tcacacattg gtcgtagccc gtcactgttt gttaatgccg ctacctgggg gctgttgttt
     9301 actggcaaac tggtttccac ccataacgaa gccagcctct cccgctcgct gaaccgcatt
     9361 atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg atatggcgat gcgcctgatg
     9421 ggcgagcagt tcgtcacggg tgaaaccatc gcggaagcgt tagccaatgc ccgcaagctg
     9481 gaagagaaag gtttccgtta ctcttacgat atgctgggcg aagctgcact gactgccgca
     9541 gacgcacagg cgtatatggt ttcctatcag caggcgattc acgccattgg taaagcgtct
     9601 aacggtcgtg gcatctatga aggtccgggc atttcaatca agctgtcggc gctgcatccg
     9661 cgctacagcc gcgcccagta tgaccgggta atggaagagc tttacccgcg tctgaaatca
     9721 ctcaccctgc tggcgcgtca gtatgatatt ggtattaaca tcgatgccga agaggccgat
     9781 cgtctggaga tctccctcga tctgctggaa aatctctgtt tcgagccgga actggcaggc
     9841 tggaacggca tcggttttgt tattcaggct tatcaaaaac gctgcccgtt ggtgatcgat
     9901 tacctggttg atctcgccac ccgcagccgt cgccgtctga tgattcgcct ggtgaaaggc
     9961 gcgtactggg atagtgaaat caagcgtgcg cagatggacg gccttgaagg ctatccggtt
    10021 tatacccgca aggtgtatac cgacgtttct tatctcgcct gtgcgaaaaa gctgctggcg
    10081 gtgccgaatc taatctaccc gcagttcgcg acgcacaacg cccatacgct ggcagcgatt
    10141 tatcaactgg caggtcagaa ctactatccg ggtcagtacg agttccagtg cctgcatggt
    10201 atgggcgagc cactgtacga gcaggtcaca gggaaagttg ccgacggtaa acttaaccga
    10261 ccgtgtcgta tttatgcccc ggttggcaca tatgaaacgc tgctggcgta tctggtgcgt
    10321 cgcctgctgg aaaacggtgc taacacctcg tttgttaacc gtattgccga cacctctttg
    10381 ccactggatg aactggtcgc cgatccggtc actgctgtag aaaaactggc gcaacaggaa
    10441 gggcaaactg gattaccgca tccgaaaatt cccctgccgc gcgatcttta cggtcacggg
    10501 cgcgacaact cggcagggct ggatctcgct aacgaacacc gtctggcctc gctctcctct
    10561 gccctgctca atagtgcact gcaaaaatgg caggccttgc caatgctgga acaaccggta
    10621 gcggcaggtg agatgtcgcc cgttattaac cctgcggaac cgaaagatat tgtgggcttt
    10681 gtgcgtgaag ccacgccgcg tgaagtagaa caggcgctgg aaagtgcggt taataacgcg
    10741 ccaatctggt ttgccacgcc tccggctgaa cgcgcggcga ttttgcatcg cgctgccgtg
    10801 ctaatggaaa gccagatgca gcaactgatt ggtattctgg tgcgtgaagc cggaaaaacc
    10861 ttcagtaacg ctattgccga agtgcgcgaa gcggtcgatt ttctccacta ctacgccgga
    10921 caggtgcggg atgatttcgc taacgaaacc caccgtccat tagggcctgt ggtgtgtatc
    10981 agtccgtgga acttcccgct ggctattttc accgggcaga tcgctgccgc actggcggca
    11041 ggtaacagcg tgctggcaaa accggcagaa caaacgccgc tgattgccgc gcaagggatc
    11101 gccattttgc tggaagcggg tgtaccccct ggcgtggtgc aattgctgcc aggtcagggg
    11161 gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtgc gcggggtgat gtttaccggt
    11221 tcaaccgaag tcgctacgtt actgcagtgc aatatcgcca gccgcctgga cgctcagggt
    11281 cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg cgatgattgt cgattcttca
    11341 gcactgaccg aacaagtcgt cgtggatgtg ctggcctcgg cgttcgacag tgcgggtcag
    11401 cgttgttcgg cactgcgcgt gctgtgcctg caagatgaga ttgccgacca cacgttgaaa
    11461 atgctgcgcg gtgcaatggc cgaatgccgg atgggtaatc cgggtcgcct gaccaccgat
    11521 atcggtccgg tgattgatag cgaagcgaaa gccaatattg agcgccatat tcagaccatg
    11581 cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa acagcgaaga tgcccgtgaa
    11641 tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac tggatgactt tgccgaattg
    11701 cacaaagagg tctttggtcc ggtgctgcat gtggtgcgtt acaaccgtaa ccagctacca
    11761 gagctgatcg agcagattaa cgcttccggc tatggtctga cgcttggtgt ccatacgcgt
    11821 attgatgaaa ccatcgccca ggtcactggc tcggcccatg ttggtaacct gtatgttaac
    11881 cgtaatatgg tgggcgcagt ggttggtgtg cagccgttcg gcggcgaagg gttgtccggt
    11941 accgggccga aagcaggcgg tccgctctat ctctaccgtc tgctggcgaa tcgcccggaa
    12001 agtgcgctgg cagtgacgct cgcgcgtcag gatgcagagt atccggtcga tgcacagttg
    12061 aaagccgcat tgactcagcc gctaaatgca ctgcgggaat gggcagcaaa tcgtccagaa
    12121 ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg caggaataca acgattgctg
    12181 ccggggccga cgggtgaacg caacacctgg acgctgctgc cgcgtgagcg cgtgttgtgt
    12241 attgccgatg atgagcagga tgcgctgact cagctcgccg ccgtgctggc ggtgggcagc
    12301 caggtactgt ggccggatga cgcgctgcat cgtcagctag taaaggcatt gccatcggca
    12361 gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa ccgctcaacc ctttgatgcg
    12421 gtgatcttcc acggtgattc ggatcagctt cgcgcattgt gtgaagccgt tgctgcgcgg
    12481 gatggagcaa ttgtttcggt gcagggtttt gcccgtggcg aaagcaatat ccttctggaa
    12541 cggctgtata tcgaacgttc gctgagtgtg aataccgctg ccgctggcgg taacgccagt
    12601 ttaatgacga tcggttaaac aggtagccgg aggatgttac accctcctcc ggcatcttta
    12661 acgtggtcga atcccctcaa taataatccg ctgcacgttt tcaaccgttt gattgaaaaa
    12721 tacctcatcg cgcaacgttg cgcctgtcac cgcctccacc tgaggggcga aatcggcgta
    12781 atgttgagtg gaagcccaaa tcataaaaat caaatgctgc ggatcaatcg gcgcgagttt
    12841 gccgcttttg acccaaccgg caatcagcgc cgatttctca tcaattaatg ccttcaaatc
    12901 gcccgtcagt tcatccatta acagcggcgc gcctgccagc atctccatac agaacaggcg
    12961 cgaagcctgc ggataatcgc gtgagacttc cagcttcaga cggatgtact ctttgatcgc
    13021 cgccagcggg gcgaaatctt cacgaaacgc ttttaacggt gccagccaga tatcgagaat
    13081 ctgccgcagc acggcaatat acagcgcctc ttttgacgga aagtaataca gcagattggt
    13141 ttttgaaaca cccgccaact ctgcgatctg ctccagcctt gtgccgtgaa aaccgaattg
    13201 tgaaaaagtg tccagtgctg cgctaagaat cgcttttttc ttcgcgctta ctgcgcgcga
    13261 acgtttaccc gttgttttca ctgcgccttg cgtcatgcgc tctcccctct tgtagattgg
    13321 caacaggata gcaaaacccc cgcccggcct cgaccttctg cactctcatc gcgcacggat
    13381 gcatgtttta tgtgcaactg ttttgaccgt ttagtccact ttttaccaga tattttagat
    13441 aacacaatgc taacacatta ataacattca ctttttaaaa actggcatcc gctttgcaaa
    13501 caagccaata cgcggctgaa aaggatatgc aggatgcagc gccccgtctg actttcactt
    13561 tacgagacga agagaggttg atgatgaaaa ttggcgtatt cgtacctatt ggcaacaacg
    13621 gctggctcat ttcgacccac gcgccgcagt acatgccgac ctttgaactg aataaagcca
    13681 ttgtgcaaaa agcggagcat taccatttcg atttcgccct
//
LOCUS       DAJTNN010000104        13564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTNN010000104 DAJTNN010000000
VERSION     DAJTNN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.104"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(231..1604)
                     /gene="cobA"
                     /locus_tag="OO150_002706"
     CDS             complement(231..1604)
                     /gene="cobA"
                     /locus_tag="OO150_002706"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5516795.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1623..2429)
                     /gene="nirC"
                     /locus_tag="OO150_002707"
     CDS             complement(1623..2429)
                     /gene="nirC"
                     /locus_tag="OO150_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5516796.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            complement(2556..2882)
                     /gene="nirD"
                     /locus_tag="OO150_002708"
     CDS             complement(2556..2882)
                     /gene="nirD"
                     /locus_tag="OO150_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5516797.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2879..5422)
                     /gene="nirB"
                     /locus_tag="OO150_002709"
     CDS             complement(2879..5422)
                     /gene="nirB"
                     /locus_tag="OO150_002709"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5516798.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRIFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5684..6865)
                     /gene="tsgA"
                     /locus_tag="OO150_002710"
     CDS             complement(5684..6865)
                     /gene="tsgA"
                     /locus_tag="OO150_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5516799.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7136..7708
                     /gene="ppiA"
                     /locus_tag="OO150_002711"
                     /gene_synonym="rotA"
     CDS             7136..7708
                     /gene="ppiA"
                     /locus_tag="OO150_002711"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5516800.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGAPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7813..7980
                     /locus_tag="OO150_002712"
     CDS             7813..7980
                     /locus_tag="OO150_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5516801.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            7970..8572
                     /locus_tag="OO150_002713"
     CDS             7970..8572
                     /locus_tag="OO150_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5516802.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8604..9167
                     /gene="pabA"
                     /locus_tag="OO150_002714"
     CDS             8604..9167
                     /gene="pabA"
                     /locus_tag="OO150_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5516803.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            9253..10473
                     /gene="argD/dapC"
                     /locus_tag="OO150_002715"
     CDS             9253..10473
                     /gene="argD/dapC"
                     /locus_tag="OO150_002715"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5516804.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(10540..12630)
                     /locus_tag="OO150_002716"
     CDS             complement(10540..12630)
                     /locus_tag="OO150_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5516805.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12681..13313)
                     /gene="crp"
                     /locus_tag="OO150_002717"
     CDS             complement(12681..13313)
                     /gene="crp"
                     /locus_tag="OO150_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5516806.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
BASE COUNT         3284 a         3490 c         3552 g         3238 t
ORIGIN      
        1 ttatatatta attaatattc attgattata tgaatataag cagactgctc gttaagaacg
       61 atacaggaat atttaattgg cgtcagtgag cgaatgactt aattttgtaa gtcgctgtaa
      121 cggtgaatat aacccattag cagataatat ttattaaggg gttatgtttt atttgctgaa
      181 tatataaaaa gagtcattaa gaaggccaga attctctggc ccgggttaaa ttaatggttg
      241 gagaaccagt tcagtttatc gcgcaggcca acaacccgac caataataat tagcgatgga
      301 ctgttcattt gctgagcaag ttcaccgagt tgcgtgagcg taccgtcaat cacgcgctgc
      361 gtgactgccg ttccgttttc gacaattgcc accggcattt cgccaggcat accgtgttca
      421 atcagctttt gctgaatagt cgcggcctga ttcaacccca tatagaacac cagcgtctgt
      481 ttttctgccg ccaggttttc ccagtccagc tcgccaccgg tttttaagtg tccggtaatt
      541 aagcgtacgc tctgggcata atcgcgatgc gtgagtggaa tacccgaata ggcagagcaa
      601 ccagaagctg cggtaatacc cggaaccacc gagaacggaa tacccgcgtt gcacagtgtt
      661 tccagctctt cgccaccacg gccaaaaata aacggatcgc cacctttcag ccgcaccacg
      721 cgtttgcctt tttgcgcttc ccgcagcagg atctggttaa tctcttcctg cggtacacag
      781 tggtatcccg cgcgtttgcc gacgaaaacg cgatcagcat cgcggcgtac cagattcata
      841 atatcgtcag aaaccagacg gtcgtagacc accacatctg cctgctgaat ttgttgcagt
      901 cctttcagcg tcagcagccc ggcatcgccc ggacctgcac caaccagcac cacttcaccg
      961 cgatggtcga gcggttcgtt gattaactgt tcggtcgttt cagtaatggc tttctgatcg
     1021 ttgtttgcca gcgactgcgc caggcggtcg ttaacgaaca atttctccca gaaacggcga
     1081 cgctcactca tcgtggtgaa ctgttgtttc actcgcccgc gtaattgccc ggcatatttc
     1141 gccacctggc ccagatgcaa cggcagcagt gattcgagtt tttcgcgcaa caggcgcgcc
     1201 agaaccggag aggtgccgcc agaggagacc gctaccatta gcggtgagcg gtcaataatc
     1261 gacggcataa taaagctggc ggctttcggc gcatcgacta cgttacagaa gatgcgacga
     1321 gcttcagcgg cttcgctgac gcgctggtta agcgtgtcat catccgtcgc tgcaatcgcc
     1381 agccagcagg tgtcgagaag gctttcatca aatggccctt cgacgagggt taacatgcct
     1441 gcatctgccc atgcggtgaa ctgtggaata aacgctaatg cattcaccgt taagcgagcg
     1501 cctgcgtcta acagcaacct tgctttgcgt tccgcgacat caccaccgcc gacaatcaga
     1561 cagtcgcgat cgcgtaattg gcaaaatata ggcaaatgat ccacgtaaaa accccttagt
     1621 aattaaccgg cagccgtttc agtttgatta aatttgtccg caaccggacg attcgatttc
     1681 ggcgtagcat accaataacc caatcccatg aatacggcac ctgataaagt attacccagc
     1741 gtcacccaca gcaggttatg accaataccc gccagcgtgt aggcttcgct gtggttgccg
     1801 aaccaggaga gcgcgaacag cgtcatgtta gcgatagagt gctcgtagcc ggacgcgata
     1861 aatgccagca gacaccacca gatagcgata aatttcgccg ccccttcagt gcgcagcgcc
     1921 atccagattg ccaggcaaac cagccagtta cacaatgcac ctttgaagaa gagtaccatt
     1981 gccggtgcag tggttttagc cagcgcgacg gagtgaacga tgctggtatc taccggcagc
     2041 aggctaccgc cgccccagct atagagcatg gcaacgaaga cggaaccgac caggttaccc
     2101 agccaggttt gcggcaggat tgcccacatt tgcccgtggc tgatgctgcc cgcttttacc
     2161 ccaaaggtga ggaacatggt gtgtccggtg aacagttcag aaccggcgat aatcaccagc
     2221 gttaaggcga taccaaaggt cgcgcccatc accagcggac gtacggacgg gtcgagcaaa
     2281 ttaccgagcg tgaaaatcaa gatgatccca agacccacat acgcgcccgc catggcggag
     2341 ctgacccaaa agccgagcgg gttatttgcc gacaggcgtg caatgcgcgc agcgttagcc
     2401 gcacacttat taatagtgtc tgtaaacatt tgattatcct ttaaaaaata aaaacaaaaa
     2461 aataaaacaa aaagtttaaa aaatgtcctg caatataccg aaggtaatta caggaccatg
     2521 caaaaagggg aggcattgcg cctcccgtta aaacattaac cgcgcagctg caccacgccg
     2581 tctttcactc gtgcttcgta atgtttgacg gaaaactgtt cgtcttccat gcacaagcca
     2641 tcacttaagc gaaaacgctg ttttttcagc gggctggcga cccacagctc gccctggtgt
     2701 tccgcaatca gcccgcgtga cagcacgctg gactcgaaga acggatcgat gttgctgatc
     2761 gcaaacacct ggtcgctgtg atacgggcgg aaaatcgcga cctgctcgtc gcctaacagc
     2821 gcgcagacgc cggtttcagg caggatgtca tcgattttgc agatgtcttt ccactggctc
     2881 atgcgttgtc ctccaccaga gttaccggga tacgttcata cggcgttgcc ggacggtgct
     2941 gttcgcgctc tggcaccatc tgcacgttcg ggtcacgctt gtcgctgttg atgaagtgtt
     3001 tgaagcgagt ctgcgcggac ggtgtattga ccgtttcagt ccactcacac actaccgctt
     3061 cacgcaggcg cgccatctct tcttccagat gagcgttcag ccccagtttg tcgtcaatga
     3121 tcaccgcttt caggtaatcg atgccgcctt cgaggttttc taaccacggc gcggtacgcg
     3181 tcagtttgtc ggcggtacgg atgtagaaca tcatgaagcg gtcgagatat ttgatcagcg
     3241 tttcgcgatc gatatcagct gccagcagat ccgcatgacg cggtttcatg ccgccgttgc
     3301 cgcaaacata caggttccag cctttttcag tggcgataat acccacgtct ttaccctgag
     3361 cttctgaaca ttcacgggta cagccggaga caccgaactt cattttgtgc ggcgtacgga
     3421 tgcctttgta gcggttttcc agttccacgc cgaggccgac gctgtcgcca acgccgtagc
     3481 ggcaccaggt gctacccacg caggttttcg ccatacgcag tgctttcgca taggcatgac
     3541 cggtttcgaa gccggcttca atcagctgac gccagatctc tggcagatcg tctttctgtg
     3601 cgccaaacat cgccaggcgc tgggaaccgg tgattttggt gtagagatta aattcacgcg
     3661 cgatacgacc cactgccatt agcccttccg gggtgatttc accgcccgga gaacgcggga
     3721 tcaccgagta ggtcccgtct ttctggatgt tagcgaggaa gttgtcgtta gaatcttgca
     3781 gcggagtatg ttctggcttc agaatgtatt cgttccagca ggaggccagc agcgaaccga
     3841 cggtcggttt acaaacttca caaccgtagc ctttgccgtg tttcgccagc agttcttcga
     3901 aggttttaat gccttcaacg cggatcaaat ggaacagttc ctgacgcgaa taagcaaagt
     3961 gttcgcacag gttgttgtta acttcgatgc cctgtttcgc cagttccgcg ttcagtacct
     4021 gagtgaccag cgggatacag ccaccgcagc cagtacccgc tttggtttca gctttcagcg
     4081 ccgcaactgt gtggcagcct ttgttgatgg cagcaatcag atcaccttta gtgacgtcga
     4141 agcaggagca gatttgcgcg ctgtccggca gtttatcaac accgatagac ggcttaccgc
     4201 tacccgagtg cgccggcaga atcagagaat ccgggttttc cggcagctcg atagcattca
     4261 gcaccagttg cagcaagtta ccgtagtcgc tggtatcgcc caccagtacc gcaccgagca
     4321 gggttttgtt gtcttcgctg acgatcaggc gtttgtagat ctctttgctt tcgtcgaggt
     4381 aaacgtagct acgtgcgcca ggcgtgcgac catgagcatc accaataccg cctacgtcca
     4441 cgcccagcag tttcagcttg gcgctaaggt cagcaccttc aaaggcgttt tcgctaccga
     4501 gaatatggtc aacggcgacc tgcgccattt tgtagccagg tgctaccaga ccaaatatac
     4561 ggttgttcca gcttgcgcat tcaccgatag cgtagatatc cggatcggaa gtttggcagg
     4621 aatcattaat gacgataccc ccacgcggag caacgtccag accacactgg gttgccagct
     4681 tatcgcgcgg acggataccg gtagagaaga cgataaagtc gacttccagt tcgctgccgt
     4741 cggcaaaacg catggtttta cgtgcttcga caccttcctg cacaatctca agggtgtttt
     4801 tgctggtgtg aacgcgcacg cccatacttt cgattttgcg acgcagctgc tcgccaccca
     4861 tctgatcaag ctgttccgcc atcagcatag gggcaaattc gataacgtgg gtttcaatac
     4921 ctaagttttt cagcgcgcca gcggcttcca gacctaacag gccgccacca acaacggcac
     4981 cgcgtttgct gcgacgggcg caggattcaa tggcgttgag gtcttcaatg gtgcgataga
     5041 caaagcagtc ctgagtatca gaacctttga ttggtgggat ccacgggtag gaaccggttg
     5101 ccatgatcag cttgtcataa aaaacggtac gtccggcgct ggagtgaatc accttctcct
     5161 gacggttgat ggtgatagcg cgttcgccga ccagaacttt gatgccgtgt ttctcgtaga
     5221 agccttcgcg caccagcgac agctcttcgg cggtgtggtg agagaagtaa gacgagaggt
     5281 gtacgcggtc ataagcgatg cgcggttctt cacagaaaac ggtaatatca aagttggccg
     5341 catcagattt atcaagaaga tcttcgataa agcgatggcc gaccataccg ttaccgataa
     5401 ttgcgagtct gactttgctc atttttgcct cgatttcttt tctattaccg cctaccttaa
     5461 cgattcagca accccgctta ttgatgtaaa tcaaattcac ctttatatac cccttaaggg
     5521 gtatgttgct gatttgtata aattttctta agtcacggaa ttgtctgaca tttcatgttt
     5581 gtgcaaaaaa tagacaaaat gaacgggttt atagaaatag atgcacgtag cggggttaga
     5641 gaggaaaagg ccggacgacg atagtgccgt ccggcattag caattaatga gaggtcaggg
     5701 tgttatgctg acggtgacgg ctgacgaaac ctaacaggaa gcacatcaca aagacgacag
     5761 cgtacagacc gtttgctgta agcagtgccg cctgcggacc gctatgttca acaatcgggc
     5821 cggtaaccac aaaggtcaac atagtaccga tggttccgca ggtcaggaca aagttaacca
     5881 gttttggcga cggtactttg gtctgctgtg aacccagggt gatgatggtg gtatagatcg
     5941 cgctggagaa gaagcccaga gcgagaattg accacgccat atgtgctggt gttccggtgt
     6001 taaagacgta catcagaatc gcagccagac cagccagtac ggtcagaatg cgttgcaaat
     6061 caaagaagcg aagaataaag ctgaacgccc acatgccgac catgtatgac atccagaagt
     6121 tactcaccag cgtgcccgcg tcgttcaggc tcatgcccag gcctttggca tactcaggca
     6181 cccaggagat aaaacctaac tgaccgagga tgtagcacag cgccgcaaca gagagaaaca
     6241 gtacgccgat cccccacttt tctttctcta ccggagcatc cgtttttggt gcatgtttgc
     6301 ccagtgctgg gaactcacag ccgaaggtca gaataaaaat agcgacatac accagcccga
     6361 tgcaggcata aacccagtac cactcaatgc tgcgcgccag tagaaacgcg gcgatcattg
     6421 ggaaaatcat cccagccata ctgaagaagg agtcagtaaa taacaggcgg gaaccgcgct
     6481 gacgcccttc atacatttgt gttatcagga atgtaccaat cgacatagta atgccgctga
     6541 ccaccccgag aatgaacatc gccgtcgaga acagcgccag gctgtggctg aacatcaaac
     6601 cggcaaccgc cagcaccatc aggagaaagc caaaacgtaa ctgcgttttc aacgggacga
     6661 tttccatcag ccaggcgttg aggaagatag agattaaaat gccggcgttg aggaaggtga
     6721 aggtgttact catactggaa acaggcagat tgaaataatc ggcgatattt cccatcacca
     6781 tcccggtgac aataaccaac gcaccagtca gtgcgtagga gagaaagcta atccatgtga
     6841 gcttgatgcg attgctgtta gtcatgattg gcctgcgttc aaaaataaaa tggcatagcg
     6901 ggatatgccg cgagcgggtg attttaggtg attttgtgat ctgtttaaat gttttattgc
     6961 aatcggttgc taaattgcat tttaagaagt gatcttgatc acggaataaa aagtgatcgt
     7021 caggttacat atatttcaga tacgtaaaat taggtaaagg gatggccttg ttcttgaagg
     7081 ctatttagaa tctcttcact tgcttttttt ctgctctgtt tgttaaggaa atctcatgtt
     7141 caaatcgacc ctggcggcga tggctgctgt tttcgctctt tctgctcttt ctcccgcagc
     7201 aatggcagcg aaaggggccc cgcacgtatt gttgacaacc tcagctggta acatcgaact
     7261 ggagctggat aaacaaaaag cgccagtgtc tgtgcaaaac tttgtcgatt atgtgaacag
     7321 cggtttttat aacaacacta cctttcaccg cgtcattcct ggctttatga ttcagggcgg
     7381 cggtttcacc gagcagatgc agcagaaaaa accaaacccg ccaatcaaaa atgaagccga
     7441 taacggcctg cgcaacacgc gtggcaccat cgcgatggcg cgtaccgctg acaaagatag
     7501 cgccaccagc cagttcttta tcaacgttgc cgataacgcc ttccttgacc atggtcagcg
     7561 tgatttcggt tacgcggtat ttggtaaagt ggtgaaaggc atggacgtgg ccgataagat
     7621 ttcccaggtg ccgactcatg acgttggtcc gtaccagaat gtgccgtcaa aaccggtagt
     7681 tatcctttcc gctaaagtcc tgccgtaatg atttctcgcg cgggcaatct tgcccgcgct
     7741 tctgctctcc cggcgtaacc cggatttgcc gcttatactt gtggcaaatg gacacgttca
     7801 gggaggcatc aagtgaagaa actcaccgat aagcaaaagt cccgtctctg ggagcttcag
     7861 cgtaatcgta atttccaggc cagtcgccgt cttgaaggcg tcgagatgcc tttagtcact
     7921 cttactgccg cagaggcttt agcgcgcctt gtagagctga ggaggcacta tgagcgataa
     7981 attcggcgaa gggcgcgatc cgtatcttta tccaggcctt gatatcatgc gtaaccggct
     8041 gaacatccgc cagcaacaac ggctggaaca ggccgcttac gaaatgacgg cgctgcgtgc
     8101 tgcgaccatt gagcttggtc cgctggtgcg cggtttaccg catttgcgaa ctatccatcg
     8161 ccagctgtat caggatattt tcgactgggc agggcaactg cgtgaagttg atatttatca
     8221 gggcgatacg ccgttctgcc actttgctta tatagaaaaa gagggcaatg ccctgatgca
     8281 ggatctggag gaagaaggtt atctggttgg cctggagaaa acgaagttcg tcgagcggct
     8341 ggcgcattac tattgtgaaa tcaacgtgct gcatcccttc cgggtgggaa gtggtctggc
     8401 acagcggatc ttcttcgagc aactggcgat tcatgccgga tatcaactga gctggcaggg
     8461 tatcgaaaaa gaggcctgga atcaggcaaa tcagagtggg gcaatggggg atctcaccgc
     8521 actgcagatg atatttagca aagtggtaag cgaagccggg gaatctgagt aaaatagcgc
     8581 ggttctttcg taccggagcc gccatgatcc tgcttattga taactacgat tcttttacct
     8641 ggaacctcta ccagtacttt tgtgaactgg gggcggatgt gctggttaag cgcaacgatg
     8701 cgttgacgtt ggcggatatc gacgccctta aaccacaaaa aattgtcatc tcacccggcc
     8761 cctgtacgcc agatgaagcc gggatctccc ttgacgttat tcgccactat gccgggcgct
     8821 tgccgattct tggcgtctgc ctcggtcatc aggcaatggc gcaggcattt ggcggtaaag
     8881 ttgtgcgcgc cgcaaaggtc atgcacggca aaacctcgcc gattacacat aacggtgagg
     8941 gcgtatttcg ggggctggca aatccactta ccgtgacacg ctaccattcg ctggtggtgg
     9001 aacctggctc attaccagcg tgctttgacg tgacggcctg gagcgaaacc cgcgagatta
     9061 tggggattcg ccatcgccag tgggatctgg aaggtgtgca gttccatcca gaaagtattc
     9121 ttagcgaaca aggacatcaa ctgctggcta atttcctgca tctctgattt ccgattgcca
     9181 ttcagtgatt ttttatgcat attttgtgat tataatttca tatttattta tgcgtaacag
     9241 ggtgatcatg agatggcaat tgaacaaaca gcaattacac gcgcgacttt cgatgaagtg
     9301 atcctgccga tttatgctcc ggcagagttt attccggtaa aaggtcaggg cagccgaatc
     9361 tgggatcagc aaggcaagga gtatgtcgat ttcgcgggtg gcattgcagt tacggcgttg
     9421 ggccattgcc atcctgcgct ggtgaacgcg ttaaaaaccc agggcgaaac tctgtggcat
     9481 atcagtaacg ttttcaccaa tgaaccggcg ctgcgtcttg ggcgcaaact gattgaggcg
     9541 acgtttgccg aacgcgtggt gtttatgaac tccggcacgg aagctaacga aaccgccttt
     9601 aaactggcac gccattacgc ctgtgtgcgt catagcccgt tcaaaaccaa aattattgcc
     9661 ttccataacg cttttcatgg tcgctcgctg tttaccgttt cggtgggtgg gcagccaaaa
     9721 tattccgacg gctttgggcc gaaaccggca gacatcatcc acgttccctt taacgatctc
     9781 catgcagtga aagcggtgat ggatgatcac acctgtgcgg tggtggttga gccgatccag
     9841 ggcgagggcg gtgtgacggc atcgacgcca gagtttttgc agggcttgcg cgagctgtgc
     9901 gatcaacatc aggcattatt ggtgtttgat gaagtgcagt gcgggatggg gcggactggc
     9961 gatttgtttg cttacatgta ctacggtgtg acgccggata ttctgacttc tgcgaaagcg
    10021 ttaggcggcg gcttcccgat tagcgccatg ctgaccacgg cggaaattgc ttctgcgttt
    10081 catcctggtt ctcacggttc cacctacggc ggtaatcctc tggcctgtgc agtagcgggc
    10141 gcggcgtttg atatcatcaa tacccctgaa gtgctggaag gcattcaggc gaaacgccag
    10201 cgttttgttg accatttgca gaagatcgat cagcagtacg atgtgtttag cgatattcgc
    10261 ggtatggggc tgttaattgg cgcggagctg aaaccacact acaaaggtcg ggcgcgtgat
    10321 ttcctgtatg cgggtgcaga ggttggcgta atggtgctga atgccggacc ggatgtgatg
    10381 cgtttagcac cgtcgctggt ggtggaagat gcggatatcg atgaagggat gcaacgtttc
    10441 gcccacgcgg tggcgaaggt ggttggggcg taaataagac gcatcaggca aaatatgtcg
    10501 gatgcggcgt gaatgcctta tccgatggtt tagcctgtat tacgccttcg aatcccgcaa
    10561 cttgcgactc agccaaatcc cgtgatgcgg tcgctgacgc catgccatcg acgaaatggt
    10621 gtgcatggtg ttcagatgac caataacccg ctgtaaatgc tgctccagcg tacctgccgc
    10681 gccttcgtgc ggctgcatct ccggcgcatc catgatattg gcatcgccag aactacccgg
    10741 ttcgtcatac tccagtcgct gctgacaacg ctgaatggcg atttcacaag actgtaaata
    10801 ctcttgtgcc agttcaggtg gcaatgcccg gtgttcccgc gccagcgtgg tcatggcatt
    10861 aatatgctca acaataaact ggctgtgcgt tacccacagt ttcatatctg ccagataatg
    10921 gctgttaaac gccggttcct gcatcgcctg attcaatgag ttatacagag tgttatgtgc
    10981 ctgatttacc cgcattcgct gccaggccag tggcgtaggt tgcggatcct cgctaagaat
    11041 caagcgaatc gcttcctgat aggcttctaa agcgtcatgg gcgtttttac gcaataaccc
    11101 gctctgccac tgcggccaca gccagacagt accgccgaaa gcaattaaac aaccaataat
    11161 ggtatcgata agacgcggaa ggatgtattg ctcgccgttc aaccacaata gttgcagggt
    11221 atacactgcg gtaatagtaa aaccgaccgt cgcccagccg tagtttttgc gcaatatcag
    11281 gtagctggcg agggtggtaa tcagcatcaa cgtcagggtg taaccttcgg gaattttaaa
    11341 gtgcagcgcc acgcccgcaa tgattaaccc gaccacggtt cccacggagc gattcacaat
    11401 cctcagacgg gttgcgccat agccattttg tgtcaccagc aataccgtca tcaggatcca
    11461 gtacgacttc ggcagatgca gcgcggtgcc catcaggctg gcaacgctta acatcacact
    11521 gagtcgtccg gcattgcgta gcgccggaga ctttagtgac agataacttt tcagcgcagg
    11581 aagtaatggc attcgccgct gtttatcggc cagtaagtca cgggcataga gcggtttttg
    11641 ggtgcgcagc acgcgggcga tgcggctgaa atgccagtag cagaattgcc caaccggatt
    11701 atccggatgc tggcgggcga ttttttccag tgcgccaatt tgcttttcca tcgtaaaacg
    11761 cgttggcagg cgatggtaaa gaatgtcatc agccagcacg cgcaggcgag cggcgacggt
    11821 ttgcgcattc cagcggataa cttcttccgc atggctacgc tcgaccagct tttgcacctc
    11881 ttccggctga tgcaaactga ccgaaatatg ttcctgtaaa tccagcgcct cctggaaaat
    11941 acgcagcatc cgcttgtagt cagtattatt ttgcgcggaa agcatatgca tttgctgata
    12001 gcactgggta attagatcga ccgctttttg ctggcgcacc agcagcggcg gcagcgcttt
    12061 ttcagggtcg gtgtgctggg taagcaggct gtatttggct tcacaataat ctgccagttc
    12121 acggtacagc agacttagtg actcgcgcag cggttgttcg cgccagatcc agaaccaaaa
    12181 ccagttaaac aatccgtacc agagagtgcc caacgcatag atgagcaacg gttcccagac
    12241 cggcatgtat cccgccagac tgagggtaaa aatggctgcc agcagcgatg cgggaagcaa
    12301 ttttgcgtgc aatggcccca actcagcagt gacgccaagt accagcgtta atccggtcag
    12361 caaaaagggc aggggaacat cttttgccag tagcagctgt gtcagtaagc tacaggtggc
    12421 aaacagcgac gcaccaatga ttaagcgttt gaaaaaacgt ttatgaggcg tatcaaggcc
    12481 cgcaatattg caacaggcag gaacgagaga gaagagcaga ccgaatcgta attcgccaag
    12541 cattaaccca acggccacgg gcaaacatag caccagcgtt tgtcgaagtg catagttgat
    12601 atcggggtga taaatcagtc tgcgccacat cgggggaaac aaaatggcgc gctaccaggt
    12661 aacgcgccac tccgacggga ttaacgagtg ccgtaaacga cgatggtttt accgtgtgcg
    12721 gagatcaggt tctgatcttc aagcatcttc agaatgcgtc ccacggtttc acgagaacag
    12781 ccgacaatct gaccgatttc ctgacgggta attttgattt gcataccgtc cgggtgagtc
    12841 atagcgtctg gttgttttgc cagattcagc agagtctgtg caatgcggcc cgtcacgtcg
    12901 aggaacgcca ggttgcccac tttctctgaa gtgacttgca gacgacgcgc catctgtgca
    12961 gacagacgca tcagaatgtc cgggtttacc tgaatcaatt ggcgaaattt tttgtacgaa
    13021 atttcagcca cttcacaggc ggttttcgca cgtacccatg cgctacgttc ctggccctct
    13081 tcaaacaggc ccagttcgcc aataaaatca ccctgattca gataggagag gatcatttct
    13141 ttaccctctt cgtctttgat cagcactgcc acagagcctt taacgatgta gtacagcgtt
    13201 tccgcttttt caccctggtg aataagcgtg ctcttggatg ggtacttatg aatgtggcaa
    13261 tgagacaaga accattcgag agtcgggtct gtttgcggtt tgccaagcac catgcgcggt
    13321 tatcctctgt tataagcttt ctccagagcc agataacgcc gctgtctctg gattgccgaa
    13381 atatgcttcc cgctacctgg gaaggggcta tcaactgtac tgcacggtaa tgtgacgtcc
    13441 tttgcataca tgcagtacat caatgtatta ctgtagcatc ctgactgttt tagcatagct
    13501 ttcgctttgt gtctcctggt gtctcgcttc agcatgaccc aggtcgcctt ccgttgcgcg
    13561 attt
//
LOCUS       DAJTNN010000105        13437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTNN010000105 DAJTNN010000000
VERSION     DAJTNN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.105"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..1862)
                     /gene="speC"
                     /locus_tag="OO150_002718"
                     /pseudo
     CDS             complement(1..1862)
                     /gene="speC"
                     /locus_tag="OO150_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2260..2958
                     /locus_tag="OO150_002719"
     CDS             2260..2958
                     /locus_tag="OO150_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5516807.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            3064..3139
                     /locus_tag="OO150_002720"
     tRNA            3064..3139
                     /locus_tag="OO150_002720"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3097..3099,aa:Phe,seq:gaa)
     gene            complement(3288..3824)
                     /gene="yghD"
                     /locus_tag="OO150_002721"
     CDS             complement(3288..3824)
                     /gene="yghD"
                     /locus_tag="OO150_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCR5516808.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="OO150_002722"
     CDS             complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="OO150_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCR5516809.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="OO150_002723"
     CDS             complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="OO150_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCR5516810.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="OO150_002724"
     CDS             complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="OO150_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCR5516811.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="OO150_002725"
     CDS             complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="OO150_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCR5516812.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="OO150_002726"
     CDS             complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="OO150_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCR5516813.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNAC"
     gene            complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="OO150_002727"
     CDS             complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="OO150_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCR5516814.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLARTVVPNLLGNKEKA
                     NRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7984..9207)
                     /gene="gspF"
                     /locus_tag="OO150_002728"
     CDS             complement(7984..9207)
                     /gene="gspF"
                     /locus_tag="OO150_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCR5516815.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYIIASGEQRGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9207..10699)
                     /gene="gspE"
                     /locus_tag="OO150_002729"
                     /pseudo
     CDS             complement(9207..10699)
                     /gene="gspE"
                     /locus_tag="OO150_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10699..12759)
                     /gene="gspD"
                     /locus_tag="OO150_002730"
                     /pseudo
     CDS             complement(10699..12759)
                     /gene="gspD"
                     /locus_tag="OO150_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12789..13437)
                     /gene="gspC"
                     /locus_tag="OO150_002731"
                     /pseudo
     CDS             complement(12789..13437)
                     /gene="gspC"
                     /locus_tag="OO150_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspC"
BASE COUNT         2998 a         3884 c         3470 g         3085 t
ORIGIN      
        1 tatgcgcatc ctgggggttc atcaccactg acgggaaact ctgctggcgg aacatcgctt
       61 tttgtaggtc tttgacgtcg aaactgacat acagatcgtg catctcctga cacaactggc
      121 gcaaagtgta gtcgcgatag cgcaccggat atttgttata aacgctcggc aacacctcag
      181 ccagcggcga gtcatcctca atatgctgtt caaactgcgc caacatctct accagttgtg
      241 ccagcttctc gtggctttcc gccggagtta ataagaacag aatggagttg agatcgcact
      301 tctccggcac aatgccgttc tcacgcagat agtgcgccag aatcgtcgcc ggaacgccaa
      361 agtcgctata ttcgccggtt tcggcattga tacctggtgt agtgagtaac agcttgcacg
      421 gatcaacaaa atactgatcc gcggcatatc cttcaaagcc gtgccacttc gcccccggct
      481 caaaactgaa aaaacggcgg tcgctggcta acactgatgt cggataatcc tgccacaatt
      541 tgccatcaac aacgggcggg ataaacgggc ggaacagctt acagcgcgca agaatagcct
      601 tgcgcgactc aatacccaac tcaacgcact cagcccacag ccgacgccca ctctcccctt
      661 catgaatttt ggcgttaaca tccagtgcag caaacagcgg atagaaaggg ctggtagaag
      721 catggagcat aaaggcgtta ttcaaccgct tatgcgggca aaaacgcgcc tgtccgcgga
      781 tatggttatc ttttttatgg atctgcgacg tctgtgagaa tcccgcctgc tgtttgtgca
      841 ccgactgagt cacaaagatc tccggatcgt tttcgttaag ttctaatagc agcggcgagc
      901 tatccgccat catcgggata aactgttcgt aaccgaccca cgcggaatca aacagaatgt
      961 aatcacacag atgcccaacg gtatcgatca cctgacgggc gttatagaca gttccgtcat
     1021 aggttcccag ctgaataatc gccaggcgaa acgggcgcgg cagctcggct ttttctggcg
     1081 caacgtcgcg aatttgctgg cgcagatact cttcattaaa acagtgcgca tcaataccgc
     1141 caatgaaacc aaacgggttg cgtgaagctt ccagatagac cggcgtcgcc cccgcctgaa
     1201 tcagcgcgcc gtgatgattc gacttatggt tgttacggtc gaagagcacc agatcgccac
     1261 gcgttaacag cgcattcgtc accactttat tcgctgccga tgtgccgttc agcacaaaat
     1321 aggttttatc ggcatgaaag actttggctg cgaatttctg cgcatctttc gccaataagc
     1381 agatcgccca attttacgtc agcgttacac atatcggcgc gaaagacgtt ctcaccaaag
     1441 aaatcgtaaa aatggcgtcc ggcaggatgc tttttaaaaa acgcaccatg ttgatgtcca
     1501 gggcaagcaa aggtgctgtt gcccatctca acgtactgcg tcagcgtgtc ataaaacggt
     1561 ggcagcaaat tctcttcata ctgacaggct gcggattcca gctccagcca ctgctgctcg
     1621 ttgccgttga ttaccgccgt aacgcccgca ggtaattcaa cagcatgttc ggaatacaaa
     1681 aacaccggta gatgaaaacc ggtgcgctta agcaacgcaa gaatgccact gcgactatcc
     1741 gcagcggtaa tgacgactgc cacgacgtcc gtaaaatcag tatctcccaa cgccaccacg
     1801 cgacgatgag aagaaagtcg ggataccagt tcactactgg cggcaatatt cattgatttc
     1861 ataagcgcaa acccgtttcg gggaagtaag aataccggac aaggtggaaa accctgcccc
     1921 atgagatatg ggtcaaactg gtcaccagct ccgaccgcca gacatcagta aaagcagaaa
     1981 cgctctgatt ttactgttgt cctgcagtga gcgtgcgtta acttcaccgc atgagcagta
     2041 acatagaaag ggaaacgttt cgcgcgaaac ggcgataagc gagagaatgt aaggaggtgg
     2101 cgtgcatcgg gcaaactccg tacagagagg agaaaattcg cgcaatcatg gcacctttcg
     2161 ctaaagcgtg caagccagga ctttgcgacc agacaagcca tcaacaaggc aaaaagttca
     2221 taataagaaa atcaaacaat acagttaaca ggaactcttg tggtcatcgg cccttttatc
     2281 aacgctagtg cagtcttact gggtggcgtt ctcggcgcac tgctcagcca acgcttaccg
     2341 gaacgtatcc gcatctccat gacatctatt tttggtctgg catcgctggg gattggtatt
     2401 ttactggttg tgaaatgcgc caaccttcca gcgatggttt tagctacgct acttggcgct
     2461 ctaattggcg aaatttgttt gctggagaaa ggtgtcaata cagcagtcgc caaagcacaa
     2521 aatctgtttc gtcactcacg taagaagcca acgcatgaat cttttattca gaattatgtc
     2581 gcgattattg tcctgttttg cgccagcggc accgggatct tcggggcgat gaacgaaggg
     2641 atgaccggcg atccgagtat tttaatcgcc aagtcatttc ttgatttctt tacggcgatg
     2701 atcttcgcct gctcgctggg tattgcggtg tcggtaatta gtatcccatt actgatcatc
     2761 cagttaacgc tggcgtgggc tgccgcgctg atattaccgc tgaccacacc gtcgatgatg
     2821 gcagacttca gcgccgtagg cggtttattg ctgctggcaa ccggattacg catctgtggc
     2881 attaaaatgt tcccggtggt caacatgctt ccagcactcc ttctggcaat gccgctttcc
     2941 gccgcctggt ttgcctgaca atgcgtgcaa tagcggcaaa gtgatgatag attgtgcagt
     3001 ctgcagtaaa ttgaagaaat ttgattgacg agacgaggcg aatcaggttt aatgcgcccc
     3061 gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg
     3121 attccgagtc cgggcaccac taattcttaa gaacccgccc acaaggcggg tttttgcttt
     3181 tggatctgac aataaccttc acgaaaaaag tttgcttata aagtctgggg gaattactct
     3241 cgccacgtta acgagagtaa ttttattgat actaatctcc tgatacttta cccccgtcca
     3301 aactccagcc gctggacatt caccatccca gtcttctcag cagcactggc atcaatttgt
     3361 gtcacccgca gcgcatactt ttcatccagc gcgtttaacc agttcagcag gtcattaaac
     3421 accaccggat ctatccagac ctgaatattc tccccacgct cggctatccg cttgatgact
     3481 accgagtgcg cggaagcact gtcactgata acccgcgata cctgcgcagg cgttgttgtg
     3541 gcggattttc gcgccgcaat aatatccggc gcggcgctcc tcagtctggt gttcatcgcc
     3601 accagttgct gcaacattgt ctcctgttgt actatccgtt cgctcaacgg ctgccagatg
     3661 aggacgtaat atccgacgct aaacaggaac accaccgctg ccagtaacat gcccttttca
     3721 cgcggagaac gccccgccag gtgttgtgcc agccagtgtt cgccacggct taactggcgt
     3781 tcacgccatt gctgaaaata gtgaataaat ttatcgcgta acatgttatt tcctccgcaa
     3841 cgttacgccg ccggaaaccg catcaccctc tttctgtaac gcgtcctgtt gcacaacata
     3901 atcggttgcc agtgcactac gcagtttgtc gaaactggca aagttcgcgg cccgtagctg
     3961 gaggtgaagt atctggcgtt tttgatcaaa ggtaaaacca cgcatttcga tgtcggaaag
     4021 tgacgctgat ttcagggtgc tggctatcgc tgacaactcg gcgagcagcc gggtatcgtc
     4081 ggcctgtggg cgatattttt tcagcgccat cgtcacctga gagcgtaaat tcacaatccg
     4141 cttctgctcc gggaacaacg ttaagaactg ttcctccgcc tgggtgcggc tttgcgccac
     4201 ctgttcgctg acgctccata acgtcacgcc ccgctccact gccagcgcaa ctagaatcag
     4261 caatatcggc agaatcatca cccgccagcg cgcccactgt tttcggtagc tgacgcgagg
     4321 ctgccacggc cctgtcagca ggttcccttc cggttcgcca taagtggtaa cggcgggcag
     4381 agctgtaacg gtcaggcgtt cagcgtctgc gccagcccat gctgatagct tttccggtgc
     4441 aatgccgact acggttagcg aaagcggtaa atcctgctca ttgagctggg cgcggaacat
     4501 gaccggagcc agcgcccgcc cggcgctcca tccccggcat tcatcaatgc ggcagataac
     4561 ccgttgcgca tcgccagcca taaccccaca aggaatggac atccagtctg gcgcgacggt
     4621 agcgcgggtg atgccgtttt cctgcaacca ctgcgcaatg ttgcgcatat gctgttggtg
     4681 aatcactgct acggttgcca gttgctggtc gatattcaac ggggcgaaat gcagttcatc
     4741 gatatcctgg ttcagctctt cttccagtaa ggcgggcaga atggtcggta tctgcttacg
     4801 ggcgacatca ggcagttcaa cctgccagac gctgatccat tcgccgggaa tgtagagtcg
     4861 aatcgcatcg gtttgcagcc attgctggag ctgttcgtta gcaacgtcag accagatacc
     4921 gtgctccaca tcgggtgtac ggcgctgcca atggatggga tcagcggcgc aaagcgggaa
     4981 aaaaatctca aggatggaac tcactcactt tctcctgtct gatgccagag aaccgaaaag
     5041 tgttgtgggc ccatgcggac aattaacgag ttcatcgtca gttcaatctc attcacggtg
     5101 atatctgaac gcagccagaa gtaattgctg tccacgctca ggacggtttt tagctgtttt
     5161 ttagtacgct catcgacgtc agcaagtagc ggctgtgcaa gaaactgatc gacatcttcc
     5221 cagcccttcg ccggacgttg ttgtaataac gcccgcgcct gaacggggct taaccacggg
     5281 tcaaacagcg cctcaagaat cacactttgc gtgacgtcta aagtattgat gttgatttgc
     5341 tggcggatca tcggcagcgc acagaccagc ggtttcagtt tttgataaag cccggcgtcc
     5401 attccctgta ccacgcgcat ctcactgata tcagccagcg gttgattggc ggcgtagaac
     5461 ggcaccgaac gggcgagata ctcgctgtct tcacggccca gacgtgtctg cacgctgcgg
     5521 tcctcgtcaa taaactccca caggctttcg gctatcagtt cggcccgata agcaggcaca
     5581 tccaggcgcg agatcagggc aatcaactgt tgtaccgcaa gcggacgcga cgccgttgtc
     5641 ggctgagcaa gggcattcag gttaaagcaa gcctgtgcgt cacgcagggt gaccgcaatt
     5701 tgcccctgcg gcagagggaa gaaacgaggg ccggatgccc acggctgtgc cagatgcacg
     5761 cgcttttcat ttttcaggct cagactgagc gcgctcagcg ccaggctttc cgcactggcg
     5821 ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg ttcgcccaag ttgttgctgc
     5881 attcgcccgg aaagcgtgat ggttaccagc atcataaccg ccagcaatac cagcaccacg
     5941 accagtgcca ttccgcgttt tggtggtgag gtgatcatga ttattgcggc ccgcgtaaca
     6001 accagatgcg ttcaatctcg ccccattgcg gcgaatgcag ggtcatgcgt accgccacag
     6061 ggatcgcctg tactgacgac cagctctcct gccagcgcgt gccgtcgtag aactgcaaac
     6121 ggagcgaatc ggccggaatc aacttttgca ttgtcggctt cacgctgcct gcggcatcgg
     6181 tcagcggcca ggctaaccgt tcgagataac cgtcatgaat gcggtaaccg acggtgagca
     6241 gattgctgcg cggcagacgc atcaacgggt taaccacacc gccgcgcaca aaacgcattc
     6301 cttcactctc agacgccagc acgccagcgc cagccagtaa agctggttca cgctggccct
     6361 gatcgcctct taccggacgc ggcatcattt gggtcagatc gtgggtcaga aaactcatcg
     6421 tttgctgcat gaggtttagt ttttgatcgt gatcggcgac ggcgctattc acgcgcgtga
     6481 cgccgttagt cacctgctgc gccatcagtg ccagcgaggc aaaaatggca attgccacca
     6541 acatttccag taacgtgaaa ccagcgcgag tccttctcac tgttggcctc ccacggcgct
     6601 aaaccaggcg cgtcgtgact gaatcaccga cgaaaagtct tcgtgacggc tgacttcaat
     6661 atccaccgcc tggagcagcg cgttgcctgt attcaatggg attgttcgcc agtaccagcg
     6721 gcttcctgcc atatcgctct cgccctgatg cctgaccgcc cgctgctcac gggtcatcag
     6781 gtagagcagc gcagtctggt tttcagctac ccagcccgct accgtttttt cctcaagaac
     6841 gtgctgatta ctcagcgcac cgctggcaat ctgtaacacc gccgtggcag ccagcgcaaa
     6901 aatcgccagc acgagcatca cttccagcaa ggtaaatccg cgcttcatgg catcctctca
     6961 tcacattgat tgagggataa cgcgccatcg tgtgccagct ttaccgctca acatgcgttt
     7021 tgcgccgccg agtagaagcg cagcgtaaac ggcgtggcgg gttcaaaagg cgaaaaacgg
     7081 atctgcggcg tcttcttttt cgcctctttt tgcaactcca gttcaatatc gtgcagcgtc
     7141 aggcgacgac gttgcagctc cagcgcatat tccttctgcc agatatcact tccgggttgt
     7201 aacagcatct gcacctgttt cggcaccgta acctgtgccg ataaccgggt cgcagaaacg
     7261 ggcaaccact gcccctgacg acgctgcata aactgatagc caggcgggtc gattagcaca
     7321 ccgagtgtct gtccttcaat cactgccctg tccttaaact gcgcaaagcg agtcagaaaa
     7381 tcctgtgccg cttttttcgc gggcggttca gaatcggtcg caaacgtctg taccacgccc
     7441 gcactggcaa ggccgataag gaaaatcacc agcatgattt ccagaagtgt gaatccgcgt
     7501 tcaggcactg cttactgaaa ctcctgcaaa ttccagttac cgatatctgc gccagcgcct
     7561 tccccgtttt cctgaccatc cgcccccagg gtataaacat caaacagtcc tttttcaccc
     7621 gggctgagat actgataatc attgccccac ggatcctttg gcagtcgttt gatgtatcca
     7681 ccagtacggt agttgcggga gtccgccata ttggctggct gctggatcag cgcttccagc
     7741 ccctgttctg tggtcggata acgcccgtta tccagtcggt acatatctag tgcgttttcc
     7801 agcgccacga tatcgctgat ggctttttgc cgattggctt tctctttgtt gcccaacagg
     7861 ttaggcacca ccgtccttgc caggacgcca agaataacaa tcaccaccat cacttccagc
     7921 agggtaaaac ctgcccgtgg tttgtgtgtg cgggataacg aattcatgtg ataactccgt
     7981 aaattacatt ccaaccatat tattcagttg cagcatcggc tcgaggatgg cgatgacaat
     8041 aaacagcacc acgcccgcca tcatcaccac cagcgcaggc tcaaacagcc ccaacgccag
     8101 ccccacctgg gtatcaaatt cccgttcctg gttaacagcg gcctgctcaa gcatggtttc
     8161 cagttcgcct ctctgttcgc cggaagcgat aatgtacagc atcatcggcg ggaacaggcg
     8221 caactccgcc agcgcggcgc gcagactgct tccttcgcgg acgcgatctg ccgccagcag
     8281 cagttgttgt tcgacatagc gatttgccga cacggcggca gcggtctgaa tgccttccag
     8341 tagcggaaca ctgctggcgg tgaggatgct taatgtgcgg gaaaaacgcg ccgtattcag
     8401 cccgcgcgca acacggcccg tcacgggcag acgcagcaag gttttatccc agcgcaggcg
     8461 catagtagga tttttaagta gccattgccc cagcaccaga agcgccagca atcccgccag
     8521 ccagtaaacg ccgctggcct gtaacgcatc actcatagcg ataagcgcac gggtggagga
     8581 gggtagcgcg tgtccgagat gatcaaactg ttcgatgatt ttcggcacca ctgccgtcag
     8641 caaaatagtg accacgcccg ttgccaccac cagcataacc agcggataga gcatggcctg
     8701 caatagacgt gatttcaggc gctgtcgctg ttcggtgtaa tctgccaggc gattgagcac
     8761 cacgtcgaga tgcccggatt tttctccggc agcgaccatc gaacaaaaca gggaatcaaa
     8821 gacgcgggga tgttcgcgca ggctgtccga cagggtataa ccttcctgaa tccggctgcg
     8881 cagcgccatt ccgaggctct tcacatgcag tttttcactt tgctcactga ccgcctgcaa
     8941 gcaggtttcc aacggcattg ctgcctgcac cagcgttgcc agttggcgcg tgaacagcgc
     9001 cagatctgcc gccgccacgc gacgatgtgc gtgccgccga cgctgcaaca ttccccctgc
     9061 cgatgcattc atcctggctt caatgtgcac ggggataagc tctttaccgc gcaacaactg
     9121 gcgggcatga cgcgcggaat ccgcctcaat catgcctttg gttttgcgac cattacgctc
     9181 cagcgcctga tagtaaaaca gtgccattac gcctccatgg ttacccgcag aacttcatcg
     9241 agagaggttt ctccggcgag cactttctca atgccgttgc tgcggatacc cgcagagtgt
     9301 tgccggacat aacgttccag ctccagctcc ccggcctgac ggtggatcaa atcacgcaat
     9361 gtggcatcca ccacgtcagc tcatggatgg cagtccgtcc gcggaaacct ttgtgattac
     9421 aggcggggca gccctgcgga tggtacagag tgacggttcg cgcatcggta atgcccagca
     9481 ggcgtttttc ttcgtcagtg gcaggcgcgg cctgacggca gtcgggacac agcgtgcgga
     9541 ccagtcgctg cgccatcacg cccgtcagac tggaagagag caggaaaggc tccacgccca
     9601 tatcctgcaa acgcgtgatc gcccccaccg ccgtgttggt atgcagcgtg gaaaggacca
     9661 ggtgtccggt aagcgatgcc tgaacagcga tttctgcggt ttcggtatcg cggatttcac
     9721 cgaccatcac cacatccggg tcctgacgca aaatcgcgcg caggccacgg gcgaaggtca
     9781 tgccgacgcg ggtgttaacc tgtgtctgac cgatcccttc aatcatgtat tcgatggggt
     9841 cttcaaccgt gagaatgtta cgcgaatggt tgttcagctc ctgcaatccg gcgtacagcg
     9901 tggtgctttt gccggaaccc gtcggccccg tcaccagaaa gatgccgtgc ggtttgtgta
     9961 acagctggcg caactgcacg gtcagttgct ggctaagccc cagacgctcc agcgtcaggc
    10021 gggcctggtt tttgtccagc agtcgcagca ccacgcgctc gccccaggca gaaggcatgg
    10081 tggagacacg cacgtcaatc gcccggccgc ccagcagcag cgcaatacgt ccatcctgcg
    10141 gtacgcgctt ttcggcgata tccagccgtg ccatcacctt gatacgcgat accagcagcg
    10201 aggccagttt gcgccccggg cgcagcattt catgtaatgt gccgtcaacg cgaaaacgga
    10261 tcaccagact cttttcaaac gtctcgatgt ggatatccga agcgccttct ttaatcgcct
    10321 ctgccagcat ggcgttgatc agtttgatga tcggcgcatc gtcgtcactt tccagcagat
    10381 cttccgtttc tggcagttct tccgcgagag taaaaaagtc ctcagcagaa ccgagatctt
    10441 ccatcagctg gcgagcctcg gaagagtccc gctgccagac cgcattcagc cgctgttcaa
    10501 attcggcctc gtcgatttgc cgcagcgtaa agggcgcgtt cagcccccgc tgcagctcct
    10561 gcaaaacaga gagcgatagc ggatgaacgt ggaggatctc cagcgacgct tcgcaccatg
    10621 ccaccaggct aaaccgacgg ctgaaactgt agggcagacg cacggtcttg gcggtggttt
    10681 cctgtgctac aggcaccatt aacgcgttct cccggcattg aggaacgcgc gaacttccgg
    10741 cggtaaggcc tggttttgcg ctggcagtac cggctgtgcg gtgtgcggca tcaggcttaa
    10801 accttgctca tcacgataga tctgttcggc gcgcatatag ttatatttgc gctgcgacac
    10861 gccgtctgcc gccataccgt catgcagaat ggtcgggcgg ataaacacca tcaggttacg
    10921 tttttctttt ttatccgccg tcgatttaaa caggttacca atcaatggga tatcgcccag
    10981 cagaggcact ttcgccacgc tttctcccgc ctgatcgtcc atcagaccac caagcacaat
    11041 cagctcacca tcgttagcca gcacggtggt tttcagtttg cgctcaccaa acaccacgtc
    11101 gaggctggtc tgtccttcca ccttcgagac ttcctgctca atcaccatct gtaccgcgtt
    11161 accttcgtta atctgcggcg tgactttcag catgatgccg acttttttcc tttctactgt
    11221 attgaaagga ttgctgttat tggagccaac ggtagagccg gttaataccg gaacgtccta
    11281 gcccaccatg aagaaggctt cctggttgtc cagcgtagtg atgctcggcg tggagagaac
    11341 gttcgagctg gagtcgtttt taaccgcctg caccagtgcc atccagtcgc ctttcaccac
    11401 gccaaccgcc gtaccgctaa atccagaaag aagctgggcg agtgtggaga gatcgccgtt
    11461 ggtatccgga ttaatggtgg tagcgccgtt ttcactgatc accgtcgagc ctttctgcgg
    11521 tttcgcctga gaaatggctg cccccagcgt accgataggg atctgcgtac cattagcaaa
    11581 ctgcattaat ccggcatctt tcgatgccca ctgcacgccg aagttgatat tgctaccttc
    11641 ggcaacttcc acgatcaacg cctcgacgtg cacctgagca cggcgaatat ccagttgttc
    11701 aattacgctt tgcagcgact gcatgatgtc ctgcggcgcg gttacaatca gggcattact
    11761 gtgtttgctg gcggcgatgg atacaacctc acgcccgcta ccgaccgtgc cttccgcctc
    11821 ttctttagcc gccgtgagcg tgccgctgac ctgtttcagc acatcgacca gatcttcggc
    11881 tttgctgtat ttgagataga aaacctggct gttgccgctg cgttccattt ctgagtccag
    11941 ccgacggatc aggcggcgca ttttgtcccg cgtggccggg tcaccactga caatcacact
    12001 gttggtgcgt tcgtcggcga caatttgaga tttcagcgtc gcaggctggt tctcgccgct
    12061 gtttttcgtc aggctttcca gcacgcgggc gatttccgaa gccgaggcgt tatccagcgg
    12121 gatcacctct tcggtgcgat tacccgcgtg atccacgcgc tggatcactt ccgtcaggcg
    12181 ctccacaacg gaggcgcgtc cggtgagcat aatcacgttg gagggatcgt aattaacaac
    12241 gttgcctgag cctgcgctgt cgatcatctg gcgcagaatc ggtgccagct cgcgtaccga
    12301 cacattacgt accggcacta ctttggtgac catttcatcg cccgcgtagt tgtcgctgcc
    12361 ttcaccgacc agcggcagcg gctcgacttt cgcagcgctg gatttcacca ccttcagcac
    12421 gtcgttttcc atcggtacga cggcataccc ctgtgcttcc agcaggttaa ggaatagctg
    12481 gtaatactgg cgttcattga gcggggtcat ggtgcgaata ctcactttcc cctgtacgcc
    12541 cggccccata atgatggttt tattaaggtt agcgccgacg gtttcgatga acgatttcag
    12601 gtcggtatct ttaaaattag cggtgaaagt ggcttcttcc gcccagaccg gtgaactgca
    12661 taatgccgct gccagcacca gcggcagtaa acgctttttt gttttgaggc cagttgtctt
    12721 cttacgccag accgacaagg taatatcacg ccaaaacacg atgaatgatt ctcctggatt
    12781 aaatgcggtt agcgcagcgc gatggaaatg tcgtggcgcg cacccttgcg taaaaccgta
    12841 agttgaatgg aatccattga aggtaactgc cgcatcagag caatcattgc tcgtggatca
    12901 gtgaaatcct gctgatttag cgcaatggcg atatcgcctt ccttgaaacc gctggcatcg
    12961 aacagagaac gatctgcccc cggcttcact gcataaccga caattccctc cttacgcaca
    13021 ggcgtaagct ggatatagtt aaaaattttc tgcggatctt tcgccagtgc ctgacgcacg
    13081 gcagtcggta tctcaactgg cgcactgaca gcaggttcag caacagcttg ctttgcttcg
    13141 tcactgacag cttttttgtt ggtcgcggca acggtggaac gctcctcttc agccaggctc
    13201 aggcgctcta tttttccctg atagcgcagc atcacatggt cgcgattgat ttcctcaatc
    13261 accgcgttgt gcgagccaag cgtttcaccc tgcaaataga cctgctgttt accgccttct
    13321 tcaataaccg cgccgggtct ggcaccaaag gcgatcccgc gcgcaccaca ttaagacgcg
    13381 tttctgccac aggcacaggt tcgggttgtt ttaccggcgt ggcgacgggc tgatatt
//
LOCUS       DAJTNN010000106        13339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTNN010000106 DAJTNN010000000
VERSION     DAJTNN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.106"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..170
                     /locus_tag="OO150_002732"
     CDS             <1..170
                     /locus_tag="OO150_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5516816.1"
                     /translation="KDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSAEKAQSVTSV
                     ECTVTVSAAAG"
     gene            185..559
                     /locus_tag="OO150_002733"
     CDS             185..559
                     /locus_tag="OO150_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HCR5516817.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            655..864
                     /locus_tag="OO150_002734"
     CDS             655..864
                     /locus_tag="OO150_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCR5516818.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            912..1172
                     /locus_tag="OO150_002735"
     CDS             912..1172
                     /locus_tag="OO150_002735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516819.1"
                     /translation="MATLRELIIKIVSFRGTHIDAFFRRASSAAHTNARGLITVDLFC
                     CTEAIARQVPEAGSEVLSSVIASGREVLTRGLIAGDDVLTRE"
     gene            complement(1307..2908)
                     /locus_tag="OO150_002736"
     CDS             complement(1307..2908)
                     /locus_tag="OO150_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HCR5516820.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKMIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(2915..3307)
                     /gene="tnpB"
                     /locus_tag="OO150_002737"
     CDS             complement(2915..3307)
                     /gene="tnpB"
                     /locus_tag="OO150_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5516821.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTQNTLLTQ"
     gene            complement(3294..3623)
                     /locus_tag="OO150_002738"
     CDS             complement(3294..3623)
                     /locus_tag="OO150_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5516822.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            3668..6928
                     /locus_tag="OO150_002739"
     CDS             3668..6928
                     /locus_tag="OO150_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5516823.1"
                     /translation="MTEDPCEDDFPVTTLKISANSQSFQSEIQRASRMGSEYYRTLQN
                     GGRHAAAAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATEHLISLADEWGSVNA
                     RLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVT
                     EAISTGLKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGV
                     ARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSGSITKVENAFMAWVGE
                     VNEACGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTA
                     ARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNR
                     NIAARSAAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQAWESA
                     RQYALTIDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQ
                     YVLANPGWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLALMQEKAQSIQDVLA
                     GLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRL
                     PQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYIS
                     NALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTA
                     QGELKTLTEMQKQELLRNAAQIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQ
                     GERLRERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQE
                     AFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNK
                     VEWRSWASSVLQEISKVLMNAAIVNGIKTAANSMSGAGGFIGSIGSWLGGAVANAKGG
                     VYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMN
                     GSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            6921..7262
                     /locus_tag="OO150_002740"
     CDS             6921..7262
                     /locus_tag="OO150_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5516824.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            7262..7960
                     /locus_tag="OO150_002741"
     CDS             7262..7960
                     /locus_tag="OO150_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5516825.1"
                     /translation="MQDIHEESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            7971..8714
                     /locus_tag="OO150_002742"
     CDS             7971..8714
                     /locus_tag="OO150_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5516826.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAPPGDILLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            8612..9292
                     /locus_tag="OO150_002743"
     CDS             8612..9292
                     /locus_tag="OO150_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5516827.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGSFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            9533..13006
                     /locus_tag="OO150_002744"
     CDS             9533..13006
                     /locus_tag="OO150_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5516828.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPESFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKCLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTLGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTVTVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSCVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGISIS"
     gene            13074..>13339
                     /locus_tag="OO150_002745"
     CDS             13074..>13339
                     /locus_tag="OO150_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5516829.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWH"
BASE COUNT         3029 a         3320 c         4172 g         2818 t
ORIGIN      
        1 agaaggatgg tcagccggtt gacgggcaga cgacagacac cttcagtaag ccaggtgcgc
       61 agtccgctga tgcggggaaa tatacctgcg tggtgaccga ttcggcagag aaagcacaga
      121 gtgtgacgtc tgttgaatgc accgtgacag tgagcgcagc tgccggataa ggggatgggt
      181 catcatgaaa aaggatctga aaacgctggc gctggccaga ctgtcagggt ttcgtcataa
      241 aacggtgaag gtgccggaat gggggaatgt cagcgtggtg ctgcgggagc cttcggcaga
      301 ggcctggtat ctgtggcagg aagtgctcaa tggtgatgga gaggatgacg ataccctgtc
      361 ggtggtggcg aaaacccgcc gtaacttgga agcggatgtg acgctgttct gcgatgtcct
      421 gtgtgatacg gacctgcagc gggtgttcac tccggacgac cgtgagcagg tgctggccgt
      481 ctatggtccg gtacatgccc ggttgctgcg tcaggcactg gaactgatcg ctgatgcaga
      541 gtcggccaga aaaaagtagc ccgcccggaa attcgctttc tgatgcgact tgcgctccgt
      601 ctggggcgca ccttatccga actgcgccac agcctgagtg cgagcgaggc gatgatgtgg
      661 atggagttcg acaggatatc cccgctgggt gatgagcgcg gggatatccg taatgcacag
      721 atcgtgaaag cggttttcgg ggcacagggg atgaatgttg cactgaagga cgtcatgctc
      781 tgctggggcg aggatgagga taagccggag gtggatccgt ttgcggcgct ggaagacgcg
      841 ctgagctttg cagcacagtc atgaatgatg agaaccgctg aggcggtttt tttacgcccg
      901 gagaaaggtg aatggcgacg ttacgtgaac tgattatcaa aattgtaagc ttccggggaa
      961 ctcatattga cgcgtttttc aggcgcgcgt catcggcggc gcatacgaac gctcgtggtt
     1021 tgataacggt tgatttattt tgttgcacag aggcgatcgc caggcaggta ccggaggcgg
     1081 gctctgaagt actgagtagc gtgatcgcgt caggccgcga agtactgact cggggattaa
     1141 ttgcaggtga tgatgtactg acccgggaat gactccgggc tgattcaggt tcttattcgc
     1201 tgtcagcccg cgtggttgct ggtctggctc atttcctgca gtcaggagtt caactcccga
     1261 ctgcaggtta tggcccggtg cacgaacacc tgacggcagg tatcactcac ccggagaagg
     1321 taaatccctc aagaggcagc aactctgcca gtcgctcctc cggccactcc ggcagacgca
     1381 tcaggacgtc tgtcaaccag gcatgcggct ccagaccgtt gcgtttcgcg gtttccagca
     1441 agctcattat ttgcgcggcg cgttctcccg ccatctgcga accggcgaac agccacgatt
     1501 ttctgcccag aaccacgttt ttgatggccc gttcacacac attattatcc agcggcaccg
     1561 caccatcttc caggaagcgg ctcagttcca cgcgatgaga cagcgcatag gcaatagctt
     1621 tgtgtaatgc ctttcccgga gaacattgcg gttcctgctc ttcaagccat gaccacagtt
     1681 cttccagtat cggacgggca taacgctgtc gccactggcg gattttttcc acggggcggc
     1741 tactgatctt cttctcaaga cggtacaacc ccgcgatttt cttcacggct atggcagccc
     1801 gtggatcttt actgatttta tacaggtcgg cgaagcccct gcgggcatgg gcccagcatc
     1861 cggccagcgt gatctccggc actttgtttg ccagagtccg gtaagcttta tgtccgtcga
     1921 caaccagcgt cccgccccag ccctgaagcc agttttcagg atactcatgg ctacgtccgg
     1981 tccggcagtc gaagcacaca actgacggtc ccgtcctttc tccactgacg tatgcccaca
     2041 gataaccgga gcaggattta ccgcctttct tcgtgttcag gatcttcagg gtagtctcat
     2101 ctgcatgcac caccggacgg tttatcaact cgcgatggag taacgccgcc aggggagata
     2161 atgcggcacc cgccgcgcca accatgtcag ccatcgaact gacgggcagc ccgacatctg
     2221 aacgggcaaa gacctgttgc tggcgataca gaggcaggtg gtcaccgtat ttattgatga
     2281 tcacctgtgc gatgactgat ggctcaacgg cacttttcgg gaggatatgt gccggcattt
     2341 caccgctgaa caccttctgg caacagggac aactgtattg cggacggaca taacggttca
     2401 ccacaaagtg agcgggaata tactccagct tttcactcac cgcatcgcgg atatagtgca
     2461 gcggctcgtc acattcaggg caatggtcag tgtccggctg gatcattttt tccacccgtg
     2521 gaagatgaac cggtaagggt ttgcgtatcg ggcgtgaacg ggacgctttt tcatcttctt
     2581 caggggattg cgggagcagt ttatccagat gtgcggtaag cgcggcgata tcggcatcaa
     2641 catcctcttc gaatagggag cgttgcatac cagccagcgt ttcacatttt tttccgaagc
     2701 gctgttgacg aaccagtttc agcatttctt ccagaagctg gatgcgccgg cttttctcca
     2761 gtaattcccg ctctttttcg gcattctccg acatgacttc ttgtaccatg gcaagtgcca
     2821 tagcacgcag tttttcgatg tcattcgtgg tgttgagagc ggagatatcc atacagggga
     2881 gtataccact ccagatacgg atattcatta tttttcactg agttagcagt gtattttgcg
     2941 tgttttcagg gcgcagttca ggttcattct gccagaccca tttcgtcagg tcatgtccct
     3001 taacctgctg ccagtcaaca ccggcaatca gccagttaaa ctcgtcggga gtgaggtgcc
     3061 aggcagcgtc atttgcacgt ggccagcgga agctgccttt gtgcagacgg cgggtacaca
     3121 accacacccc gtgtttatcc caccggagaa ctttgatacg cgaacggact ttgttaacaa
     3181 agacgaaggc ggctccctca tgccatgtcg accggagttc atcctgtata tactgcgtca
     3241 gtgagtcgat gccccggcgc atgtcaacag gtttaattgc gatgaagaca ttatcaggac
     3301 tgagcatatt tgagtgccct gaagacatcg gtaagttgtg acggctgaca gcacatccgt
     3361 actccaccgg gaaggaagag cgtgactggc tcattcgtga cgggctgtgg tacatcagca
     3421 tgcagtggtg gcgcaatatg aacgggaaga acggtcggtt catttgcccg gcgttcagct
     3481 tttgcgacat cctgcggcca ttcgcgaagt gatgtgaagg gaatatcgtt gagttcacag
     3541 tattgctgac gtgtaagacc actggcgcgc caggcagcaa cgtgctgcct tttctgttca
     3601 atggtccatc ggggttttga catcgattat ctccggtaag ttggtgaatg gatgtcggta
     3661 atactaactg accgaagatc cctgtgaaga tgacttcccc gtaacgacac tcaaaatttc
     3721 ggcaaattca cagtcattcc agtcggagat ccagcgggcg tcccgtatgg gcagtgaata
     3781 ttaccggacc ctgcagaatg gcggacgtca tgccgctgct gccgcacggg aacagcgccg
     3841 cgcccttgca gaactgaaca gtcagttgac ggaaattcgc ggttctgctg tcggaatggc
     3901 tggcgcattt gccggtgcct ttgccaccga acacctgatt tcgctggccg atgagtgggg
     3961 ttccgtgaat gcccgtctga aacaggcatc tcagtcatcg gatgaattct cgtcatcaca
     4021 gaaagtgctg atggatatca gtcagcgaac aggtaccgcg ttttcggata atgcggccct
     4081 gtttgcccgt tcggctgcct cgatgcgtga atatggttac agtgctgatg atgtgctgaa
     4141 ggtgacggag gccatttcga caggactgaa actgtcaggg gctggagttg cggaatccgg
     4201 ttcggtgatc acccagttca gccaggcact ggcacagggt gtactgcgtg gcgaagaatt
     4261 taatgctgtt aacgaaaatg gcgaccgggt gatccgcgcg cttgctgcgg ggatgggtgt
     4321 ggcccgtaaa gatctgaagg caatggcgga tgacggaaaa ctgacagcgg ataaagtggt
     4381 ccctgcgtta atcagccagc tggggatatt acgtgatgaa tatgcggcca tgccggaaac
     4441 ggtttccggt agtatcacga aggtggaaaa cgcctttatg gcctgggtgg gcgaagtgaa
     4501 tgaggcctgc ggggtgacaa aaacgctctc cggcgtgctg aacggtgttg ccggacagat
     4561 tgataatgtg gcaacagctg tgggggcgct ggttgccgtc ggggttgccc ggtactttgg
     4621 caatatggcc tccggagcga tgtctgccac ggcaggactt gtgacggctg cacgtaatga
     4681 agttgcactg gcggaagcac agttcagggg aacgcagatt gccacggcgc gggcaagggc
     4741 agccgtgtac cgtgctcagc aggccgtggc ggcagcccgc gggacggaga tgcagattgc
     4801 tgcagaggcc cgtctggcgg ccacacagga acgcctgaac agaaatattg ctgccagaag
     4861 cgccgcccag aatgcgctga acagtacaac ggcggtgggc tcacgtctga tgagcggtgc
     4921 gctggggctg gttggtggcg tacccggact ggtgatgctg ggggctgcag catggtacac
     4981 gctgtaccag aatcaggagc aggcctggga gtctgcgcgc cagtatgcac tgacgataga
     5041 tgaaatcgcg cataaaacgc cgtcaatgtc tttgcctgaa gcctcagata atgaaggacg
     5101 aacacgggcg gcgctgacag agcagaaccg gctgattgat gaacaggcta gtcgggtgaa
     5161 atccctgcag gaaaaaatcg caggatatca gtatgttctg gcgaacccgg gctggacgac
     5221 cggtgacgga ttcatgataa accatctgac ctcggtgaag accgtaacgg aagggcttgc
     5281 tcaggcaaca gagcagcttg ccgttgagca gtcccgtctg gcactgatgc aggaaaaagc
     5341 gcagtccatt caggatgtgc ttgccgggct ggaagaccgt cgtgtggcgt taattcgtca
     5401 gcaggcggca gagcagaata aggtgtacca gtccatgctg gttatgaacg gtcagcatac
     5461 ggaattcaac cgtctgctgg ggctgggtaa tgaactgctt cagcagcggc agggactggt
     5521 gaatgtgccg ttacggctgc cacaggccac tctggatgat aaacagcaga gtgccctgac
     5581 aaaaacagag cgtgagctgg ccctgtccag actgaaaggg gaagaaaaag agcgtgtccg
     5641 actggggtat gcggcggatg acctcggttt tgtgggtgat ccgtatcagg aggcgagaca
     5701 acgttatatc agtaatgccc tggaagcctg gcgcaataac gaggcgaata aacccaaatc
     5761 ccggggtgga aaatcagaga cggaaaaagc ggaagacagt ttttcccggc tgctgaagca
     5821 gcagaaagag caactggcac tggcggggca gaatacagag ctggcgaagc tgaaatacca
     5881 gactgcgcag ggcgaactga aaaccctgac ggagatgcag aagcaggaac tgctgcgcaa
     5941 tgcggcccag attgaccagc aaaaaatccg ggagcagttg cggtcccggg aagagaccct
     6001 gaagaatgat aatgtggctg ctcgtgcatc aaatgaagcg gaactgctgg ggtacgggca
     6061 gggagagcgg ctccgtgaac gcatgcggga gttgcagcag atccgcgaca gtttccgcca
     6121 gaaggatgcg gaccttcagt ctcagtatca gaccggggat atcagtgagg atttttacag
     6181 acaggctctg gcacagaacg cgcagtatct gagtgaacgt ctgaaagagc aggaagcctt
     6241 ttatgccgaa tcggatgtgc agcgtgcgga ctggcagaaa gggctgcagg agggattcag
     6301 taactgggtg gataatgcgt ccgattacgc ctcacaggca gcacagcttg cgacggaggg
     6361 tatctcaggg atggtgaata acatcacgga gatgctgaac ggaaataaag tggaatggcg
     6421 cagctgggcc tcatcagtgc tgcaggaaat atcaaaagtt cttatgaatg ccgcgattgt
     6481 caacgggatc aagacggcgg caaacagtat gtccggagcg ggaggattta tcggcagtat
     6541 tggtagctgg ctgggcggtg cggtggccaa tgcaaaaggc ggtgtgtata cctcggcaaa
     6601 cctgagtgcg tacagcaata gtattgtgga tacgcccacg tactttgcct ttgcaaaagg
     6661 ggcgggactg atgggggagg ccggtcctga agccattatg cccctgaccc gggcggcgga
     6721 tggctcgctg ggtgtgcgag cggtgggcag tatgaacggc agtgcaggtc tggtgtattc
     6781 cccggtctac catatcgcca ttcagaatga cgggactaat ggccagatag ggccggaagc
     6841 tgcgggcagc cttgtgcaac tgattgacca gcgggtgcag gcggtgatgc tgtccatgcg
     6901 acgtgacgga ggaatgctga gtggctgaga taaaaacgct gcatctggtc ccgcgtgaag
     6961 ggatgcaggt gagtgagaaa ccgtcggtgg cgagggtacg gtttggtgac ggttatgaac
     7021 agcgccgccc gacgggactt aatgcccgac tgaagacgtt tcaggcggtg ttccgggtga
     7081 cggatgaggc gacccggcga tggctggaag agtttttatc gtggcatggt ggttaccgtg
     7141 cctttttgtg gcgaccgccg aaacataacc ggacggtgag ggtggtatgc cgggagtgga
     7201 gcgtcacaga taacgccagg tacagtgatt tcagttgtac gattgagcag gtggtgaact
     7261 gatgcaggat attcacgaag aaagtctgag cgagtcggtt aaatcagagc agtcaccgcg
     7321 ggtggtactc tgggaaattg acctgacggt gcagggtggt gagcggtatt ttttctgtaa
     7381 tgagctgaat gaaaaagggg agccggtcac ctggcagggg cgtaagtatg aggcataccc
     7441 gattgacggc agcggctttg agatgaacgg ccggggcagc agtgccagac cgtcgctgac
     7501 ggtgtccaat ctgttcggtc tggtcaccgg gatggcggaa gacctgcaga gtctggtggg
     7561 ggccacggtg gtccgccgcc gggtgtatgc ccgttttctg gatgcggtga atttcgttgc
     7621 gggcaatccg gaggcggacc cggagcagga gctgagtgac cgctgggtgg tggagcagat
     7681 gtcgcagctg acagccatga cggcctcgtt tgtgctggct acaccgaccg agacggacgg
     7741 ggcgctgttt cccggtcgta tcatgctggc gaacacctgt atgtggacct accgctctga
     7801 tgagtgtggt tacacgggcg gggctgtggc ggatgagttc gataaaccca ccacggatat
     7861 ccgtaaggac agatgcagca agtgcatgcg cgggtgtgaa ctgcgcagga atgtcggcaa
     7921 ttttggcggt ttcctttcca ttaataaact ttcgcagtaa atcccgattt atgacacaga
     7981 ctgaatcagc gattctggcg catgcccggc ggtgtgcgcc tgcggagtcg tgcggcttcg
     8041 tgataagcac gccggagggg gagcggtata tcccttgtgt gaatatttct gcagagccgg
     8101 aggcgtattt tcgtatcgca ccggaagact ggctgcgggc agagatgcag ggggagattg
     8161 tggcactggt ccacagtcat cccggtgagc tgccctggct gagcgaggct gaccggcggc
     8221 tgcagataaa aagcgcactg ccctggtggc tggtctgccg gggggaaatt cataaattcc
     8281 gctgtgtgcc acatctgaca ggacggcgct ttgagcacgg ggtgacggac tgttacacgc
     8341 tgttccggga tgcataccat ctggcgggaa ttgatatgcc ggattttcat cgcgaggatg
     8401 actggtggcg caacggccag aacctgtacc tggacaatat ggcggtcacc ggcttttacc
     8461 gggtgcccct gtcctctgca ccgccgggcg atattctgct gtgctgcttt ggtgcttcgg
     8521 taccgaacca tgccaccatt tactgcggca acggtgagct gcttcaccat ctgcctgaac
     8581 aactgagtaa acgggagagg tattccgaaa aatggcaacg acgaacgcat tctgtctggc
     8641 gtcaccgcca ctggcacgca tctgccttca cggggatttg caacgatttg gccgccgcct
     8701 cagcctgtat gtgaacacgg cagcggaagc cattcgcgcc ctgtcgatgc agatgccggg
     8761 ctttcgccgt cagatgaacg aaggctggta ccagatacgt attgccggtg atgacacggc
     8821 accggaggcg gtgtatgccc gtcttcacga acagctgggt gagggaacgg tcatccacat
     8881 tgtgccgcga ctggccgggg ccggaaaggg tggactgcag attgtgttgg gggcggcagc
     8941 catcgtgggc tctttcttca ctgccggggc atcaatggcg ttatggggtt cagccctggc
     9001 agccggtagt ttttctgcca ccacgatgct gttttcactt ggagccagca tgattctggg
     9061 cggtgtggcc cagatgctgg ccccgaaggc aaaaacaccg gattaccgcg caacggataa
     9121 cggcagacag aacacgtact tttcctcgct ggataacatg attgcccagg ggaacccgat
     9181 gccggtgcct tacggggaaa tgctggttgg ctcccgccgt atatcccagg acatcagcac
     9241 ccgtgatgaa ggcgggggcg gaaaggtcgt ggttatcggg cgacagggat aaaacataaa
     9301 aaaatcccgc agtgatcgcg gagctgcggg gacagacaaa tgaagatcaa tgtgaaggag
     9361 ttgtttttgt tactcgggca aaaaaacact aacgcagcga aattataagc gccacagtca
     9421 gtgtgtgaaa atgtgaagat attcagaaat tttattccgt catgacgcag gcacccggtg
     9481 aggtgcctgt tgtttttgtg agtgaacaat tatcacggta agaggtgatg taatgggcaa
     9541 aggtggcggc agggcgcaca caccgcgtga ggcgaaagac aatctcaaat ccacgcagat
     9601 gatgagcgtg attgatgcga ttggtgaggg accgatagaa ggcccggtga aaggcctgca
     9661 gagtattctg gtgaacaaaa ccccgctgac ggacacggac ggtaatcccg tgatacacgg
     9721 tgtgaccgcg gtctggcgtg ccggggagca ggagcagaca ccaccggaaa gctttgagtc
     9781 ctccggctct gaaactgtac tgggtgtcga agtgaccagg gcaaaaccgg taacacgcac
     9841 cattacgtca gcgaacattg accgcctgcg ggtgaccttc ggggtgcagt cactggtgga
     9901 gaccacgtca aagggtgacc gtaatccgtc ctctgtccgt ctgctgattc agttacagcg
     9961 taacggtaac tgggtgacag aaaaggatgt caccattaac ggcaagacca cctcacagtt
    10021 ccttgcgtcg gtgattctgg ataatctgcc tccccgcccc tttaacatcc ggatggtcag
    10081 ggagacggcg gacagcacca cggaccagct gcagaacaga acgctgtggt cgtcatacac
    10141 cgaaatcatc gatgtgaaac agtgctaccc gaacacggcc attgtgggga tgcaggtgga
    10201 tgcggagcag tttggtggtc agcagatgac ggtgaactac catatccgcg gtcgcatcat
    10261 ccaggtgccg tcaaactatg acccggaaaa acgcacgtac agtggtatct gggacggcag
    10321 tctgaaaccg gcatacagca acaacccggc ctggtgcctg tgggacatgc tgactcaccc
    10381 gcgctacggc atgggaaaat gtctgggggc ggcggatgtg gacaagtggg cgctgtatgc
    10441 catcgggcag tactgcgacc agacggtccc ggatggtttc ggggggaccg agccgcggat
    10501 gacctttaat gcgtacctgg cacaacagcg taaggcgtgg gacgttctca gtgatttctg
    10561 ctctgcgatg cgctgtatgc cggtatggaa cggtcagacg ctgacgttcg ttcaggaccg
    10621 cccgtcggat gtggtgtggc cgtacaccaa cagcgatgtg gtggtggatg ataacggcgt
    10681 gggattccgc tacagcttca gtgccctgaa ggaccggcac acggcggtgg aggtgaatta
    10741 caccgacccg cagaacggct ggcagacctc cacggaactg gtggaagacc cggaagccat
    10801 actgcgctac ggacgcaacc tgctgaagat ggatgcgttt ggctgtacct gccgcggtca
    10861 ggctcaccgt gcaggtctgt gggtgataaa gaccggactg ctggaaacgc agacggtgga
    10921 cttcacgctc gggtcacagg ggctgcgtca cacaccgggt gacattattg aaatctgtga
    10981 taacgactat gctgggacta tgaccggcgg acgtgtcctg tccattgatg ctgccacccg
    11041 caccctgacg ctggaccgtg aggttaccct gccggagaca ggtacatcgg cggtgaacct
    11101 gattaacggc agtggtaagc cggtgagtgt ggacatcacc gcacaccccg cgccggaccg
    11161 gatacaggtc agtaccctgc ctgatggtgt ggagacatac ggggtgtggg gactctccct
    11221 gccgtcactg cgccgtcgcc tgttccgctg tgtctccgtc cgggaaaaca cggatggcac
    11281 ctttgccatc acggcggtgc agcacgtacc ggaaaaagaa gccatcgtgg ataacggtgc
    11341 ccgctttgag ccgcagtcag gctccctgaa cagcgtcatc ccaccggcag tgcagcacct
    11401 gacggtggag gtgagcgcag ctgacggcca gtatctggca caggcgaaat gggacacgcc
    11461 gcgggtggtg aagggggtgc gcttcagtct gcgactgacc agcggaagcg gagaagacag
    11521 ccgtctggtg accaccgcta tcactgcgga tacagagcat cgtttcagtg gtctgccgct
    11581 cggggaatac accctgacag tcagggcaat taacagttat ggccagcagg gcgaaccggc
    11641 caccaccacc ttccggatta acgcgccagc aaaacccgcc accattgaac tgacgctggg
    11701 gtattttcag ataacagcgg taccgcgtct tgcggtgtat gacccgacgg tacagtttga
    11761 gttctggttt tcggaggcaa aaatcgcaga cacatctcag gtggaaacct ctgcccgtta
    11821 tctggggacc ggcagtcagt ggagtgtatc cggcccgcac attaagcccg ggaaggattt
    11881 ctggttttac gtgcgcagcg tcaacctggt ggggaaatct gcttttgtgg aggccagtgg
    11941 acgggccagc aatgatgcag aagggtatct ggggctgttt cgggaaaaaa taggaaaact
    12001 gcatctggct caggggctgt gggagctgat agacaacagc cagcttgcgg atgagatggc
    12061 ggagatgaag accaccatca ccgaaacccg caatgaaatc acacagacgg tcagtaaaac
    12121 gctggaggac cagagcgcca ccatacagca gatacagcgc gtgcagaagg acacaaatga
    12181 tgaccttgct gcactttaca tgctgaaggt acagaaaaca aaaaatggca taccctatgt
    12241 tgccggtatt ggagcgggga ttgaggatac tgatggccag cccctgagca acatactgct
    12301 gctggctgac cgtattgcga tgattaaccc ggaggacggc aacaccacgc cgttatttgt
    12361 ggcgcagggg aatcagttgt tcatgaacga tgtgttcctg aagcggctgt ttgcggtgag
    12421 tatcacctcg tccggcaatc ccccgacgtt ttccctgacg ccggagggca ggctgaccgc
    12481 aagaaatgct gatatcagcg gtaacgtgaa tgcgaactcc gggacgctca acaacgtcac
    12541 gattaacgag aactgccggg ttctgggaaa actgtccgcg aaccagattg aaggcgatct
    12601 cgttaaaaca gtgggtaagg cttttccgcg ggactcccgg gcaccggagc ggtggccatc
    12661 agggaccgtt accgtcagga tttatgacga tcagcctttt gaccggcaaa ttgttattcc
    12721 ggcggtggca ttcagcggcg ctaagcatga gagagagcat actgatattt actcctcatg
    12781 ccgtctgata gtgcggaaaa acggtgctga aatttataac cgtaccgcgc tggataatac
    12841 gctgatttac agttgcgtta ttgatatgcc tgccggtcac ggtcacatga cgctggagtt
    12901 ttcggtatca gcatggctgg tgaataactg gtatcccaca gcaagtatca gcgatttgct
    12961 ggttgtggtg atgaagaaag ccaccgcagg catcagtatc agctgaattt tataacccat
    13021 atacgggcgc cagaaatggc gcctttttta ttgcagaaaa gcgagaggta attatgcgta
    13081 aagtttgtgc agccattttg tccgcagcca tctgcctgtc cgtatccggt gcgcctgcat
    13141 gggcgtctga acatcagtcc acactgagcg cggggtatct tcatgcccgt acgaacgctc
    13201 ccggcagcga taatctgaac gggattaacg tgaaataccg ttatgagttt acggacgcgc
    13261 tggggctgat tacgtccttc agttatgcca atgctgaaga tgagaaaaaa acgcactaca
    13321 gcgatacccg ctggcatga
//
LOCUS       DAJTNN010000107        13320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTNN010000107 DAJTNN010000000
VERSION     DAJTNN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.107"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            127..942
                     /gene="mepH"
                     /locus_tag="OO150_002746"
     CDS             127..942
                     /gene="mepH"
                     /locus_tag="OO150_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5516830.1"
                     /translation="MAQINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1070..1651
                     /gene="sodB"
                     /locus_tag="OO150_002747"
     CDS             1070..1651
                     /gene="sodB"
                     /locus_tag="OO150_002747"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5516831.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1797..2966)
                     /locus_tag="OO150_002748"
     CDS             complement(1797..2966)
                     /locus_tag="OO150_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5516832.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAAAFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3132..3221)
                     /gene="ynhF"
                     /locus_tag="OO150_002749"
     CDS             complement(3132..3221)
                     /gene="ynhF"
                     /locus_tag="OO150_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5516833.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3520..4545
                     /gene="purR"
                     /locus_tag="OO150_002750"
     CDS             3520..4545
                     /gene="purR"
                     /locus_tag="OO150_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5516834.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4542..5474)
                     /locus_tag="OO150_002751"
     CDS             complement(4542..5474)
                     /locus_tag="OO150_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5516835.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5587..6798
                     /locus_tag="OO150_002752"
     CDS             5587..6798
                     /locus_tag="OO150_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5516836.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7089..8237
                     /gene="cfa"
                     /locus_tag="OO150_002753"
     CDS             7089..8237
                     /gene="cfa"
                     /locus_tag="OO150_002753"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5516837.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8277..8918)
                     /gene="ribE"
                     /locus_tag="OO150_002754"
     CDS             complement(8277..8918)
                     /gene="ribE"
                     /locus_tag="OO150_002754"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5516838.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9133..10506
                     /gene="mdtK"
                     /locus_tag="OO150_002755"
     CDS             9133..10506
                     /gene="mdtK"
                     /locus_tag="OO150_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5516839.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10547..11803)
                     /locus_tag="OO150_002756"
     CDS             complement(10547..11803)
                     /locus_tag="OO150_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5516840.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12111..12187
                     /locus_tag="OO150_002757"
     tRNA            12111..12187
                     /locus_tag="OO150_002757"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12145..12147,aa:Val,seq:gac)
     gene            12192..12268
                     /locus_tag="OO150_002758"
     tRNA            12192..12268
                     /locus_tag="OO150_002758"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12226..12228,aa:Val,seq:gac)
     gene            12376..12681
                     /locus_tag="OO150_002759"
     CDS             12376..12681
                     /locus_tag="OO150_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5516841.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12807..>13320
                     /locus_tag="OO150_002760"
     CDS             12807..>13320
                     /locus_tag="OO150_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR5516842.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEE"
BASE COUNT         3285 a         3440 c         3164 g         3431 t
ORIGIN      
        1 tcgccaacga aacgtatttt ttaacaataa aagctattaa ctttctcttc ttctatgcat
       61 tagaatcatc aagttttgta aatcagacgc aggcatgata gacctgcctt aacagaggga
      121 cgctcagtgg cgcagataaa ccgtatttcg atcacgctct gtgctttact ttttaccacc
      181 ctgcctttaa cgcctatggc ccatgcttca aagcaagcca gggagagttc tgctaccact
      241 catatcacca aaaaagcaga taaaaagaaa agcacggcaa ccaccaaaaa gacccagaaa
      301 acagcgaaaa aagccgccag taaaagtacg accaaaagca aaaccgcttc ttccgttaaa
      361 aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa
      421 acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt
      481 gtgaaagtca aaaatgccgc gtcaggaact cttgccgacg cgcacaaagc gaaggtgcaa
      541 aaagctacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg
      601 ggtggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa
      661 gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca
      721 gcgccaatcg aacgtagtga actgaaaaac ggcgacctgg tctttttccg tactcaggga
      781 cgcggcacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg
      841 cgcacaggtc aggaaattca aatcacctct ctcagtgaag actactggca gcgccactat
      901 gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta
      961 cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc
     1021 acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga
     1081 attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat
     1141 cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca acctgattaa
     1201 aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt
     1261 attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa
     1321 cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt
     1381 tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac
     1441 ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac
     1501 tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcttatta
     1561 catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc tggtgaactg
     1621 ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga
     1681 tgcgcttcgt ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa
     1741 tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag
     1801 ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat
     1861 gccagtcccg cgacaatcgc tcccatcacc ggcacaaagc tgtatcccag cccagcggaa
     1921 attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg
     1981 actgatgaag acagacctgg cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc
     2041 ggtacgaccg caaaggctgc tgcgccccac accaccatgc taatagctgc gccgaactca
     2101 ttgcgggcca ggaacgggat tgccagcata atcaccatca acaacaacaa aaagcctttc
     2161 aacgtgccgt taactgaacg atctgccagt ttgccgccgg gatagttacc gatagagaat
     2221 ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt
     2281 tgcagtaccg gagagatata ggtgtagaga gtaaacattg caccagctcc cagtaccgtc
     2341 gtcagcaatg cagacaacac ctgcggacgc attaataccg ccagctcttt tttcacttca
     2401 ggtcgtgccc ctgcaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact
     2461 cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc
     2521 gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg
     2581 ctggcctgtt tatgttttgg ctccacgctt gcggccacga ctgaacccaa accaaaaaat
     2641 gctccgtgat tcaggctggt caaaatgcgt gaaagcatca gggtcatata atccggcgcg
     2701 atggcggaaa gtacgttgcc gagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg
     2761 cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc caaccattac gccaactgca
     2821 taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg
     2881 ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccaga
     2941 gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc attacgcgat
     3001 attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata acaaaaagag
     3061 aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga acgcgctccc
     3121 tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ccacgatcaa accgaggacg
     3181 ataatcgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc tcctttttta
     3241 ttaccacaca aatagtgata ttacgcattt ttacacactg tgatgaaaaa atctcccgtc
     3301 atttataatg ataagtgttt ttaccacttc cccttttcgg caagatcggc caaaattcca
     3361 cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg tcgtttttcc
     3421 ggcgtaccgc aacacttttg ttgtgcgtaa ggcgtgtaaa ggcaaacgtt taccttgcga
     3481 ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat aaaagatgta
     3541 gcgaaacgag caaacgtttc cactacaact gtgtcacacg taatcaacaa aacacgtttc
     3601 gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca ctactcccct
     3661 agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt gctggcgacc
     3721 agcagcgaag cggcctattt tgccgagatc attgaagcgg ttgaaaaaaa ttgcttccag
     3781 aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca gcgggcttat
     3841 ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc tgagtaccca
     3901 gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt gatggactgg
     3961 ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga aggcggctac
     4021 atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat ccccggcccg
     4081 ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat ggaagaagcg
     4141 atgatcaagg tgccggaaag ctggattgtt cagggtgact ttgaacccga atctggttat
     4201 cgcgccatgc agcaaatact gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc
     4261 gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggtctgcg cgtcccgcag
     4321 gatgtttcgc tgatcggtta tgataacgtg cgcaacgccc gctattttac gccggcgctg
     4381 accacgatcc accagccaaa agattcgctg ggtgaaacag cgttcaacat gctgttggat
     4441 cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg cttgattgaa
     4501 cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc cgttgcggga
     4561 gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgcc gagatactca
     4621 agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca acatgccgaa
     4681 tccgggaagg ggttttccaa ttcaagtgct acccacttcc cctcattaag ccacggtttg
     4741 gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt tgccgatgat
     4801 tcccagtcag gcacgacaac tcttttttgg ttatccagta accaggtaat acgtttaggt
     4861 agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg
     4921 aatgggccat ccatcaacgc cagcgggtgg tggctggcaa caacgcaact ccagcttagc
     4981 atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatc
     5041 gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac ttcctgaaag
     5101 acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt
     5161 tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc gttcgctatc
     5221 tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc ttctttgaga
     5281 aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa gagcggcacc
     5341 gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac gcgatgcagc
     5401 tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac ttcgagtgaa
     5461 tattctgacc acatagtctg cctgcaaaat ttttgaaatc agtcatcaaa tattaccgtt
     5521 tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga gatttagaga
     5581 atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag cgtactcggt
     5641 tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga cctgcaaacg
     5701 cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc cgcagcccag
     5761 cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt aatcggcctg
     5821 acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc tacgctgctg
     5881 gtattgcgtt ttgtacaggc tgtgggtgtc tgcgccgcgg cggttatctg gcaagcgtta
     5941 gtgacggatt attatccttc acagaaagtt aaccgaattt ttgcgaccat catgccgctg
     6001 gtgggtctat ctccggccct ggctcctctg ttaggaagct ggctgctggt ccatttttcc
     6061 tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct gcctattttc
     6121 tggctcaaac ccacgacgaa ggccggtaac aatagtcagg atggtctgac ctttaccgac
     6181 ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcggcctg ttcagccagt
     6241 ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta cagcccggca
     6301 gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg ttatggctgt
     6361 cgcgccgcgc tgcagaaatg gcaaggcaag cagttattac cgtggttgct ggtgctgttt
     6421 gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc gctggtcgaa
     6481 atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc tattgttgtc
     6541 gcccaggcgc tgcgtccctt cccacacgca actggtcgcg ccgcagcgtt gcagaacact
     6601 ctacaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat tagtatcagc
     6661 acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt cgcgctgggt
     6721 tacatgatgc aacgttgtga agaagctggc tgccagaatc atggcaatgc cgaagtcgct
     6781 catagcgaat cacactgatc tatatcgata tacttatact taggctgcta acaaaatttt
     6841 gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct acgataaata
     6901 ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat ttcggcaagg
     6961 gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt ctgcgagatt
     7021 tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg accagtgatg
     7081 gagaaactat gagttcatcg tgtatagaag aagtcagcgt accggatgac aactggtacc
     7141 gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct gccccggcgg
     7201 atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaagaaggc tctttggggt
     7261 taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg ttttttagca
     7321 aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac acgctgcgta
     7381 ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata gtcggcaaag
     7441 agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc atgcaatatt
     7501 cctgtgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg aagctcaaaa
     7561 tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt ggctgcggct
     7621 ggggcggact ggcacactac atggcatcta attatgacgt aagcatggtg ggcgtcacca
     7681 tttctgccga acagcaaaaa atggctcagg aacgctgtga aggcctggat gtcaccattt
     7741 tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct gtggggatgt
     7801 tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat cgtaatttga
     7861 aaccggaagg catattcctg ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg
     7921 atccctggat taataaatat atttttccga acggttgcct gccctctgta cgccagattg
     7981 ctcagtccag cgaaccccac tttgtgatgg aagactggca taacttcggt gctgattacg
     8041 atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa attgcggata
     8101 actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc gcaggtgctt
     8161 tccgcgcccg tgatattcag ctctggcagg tcgtgttctc acgcggtgtt gaaaacggcc
     8221 ttcgagtcgc tcgctaaagg ctattctatc gccccctctc cgggggcgat ttcagatcag
     8281 gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg ttctaccgta
     8341 tctaccactg cctgagtttg tggatcaatt tcaatgttga cgcgtgcgcc gagttttttc
     8401 ttcccaagag tcgtgcgttc cagagtttcc ggaattaaat ggacgcaaaa acgcgttggc
     8461 gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt gtacaggata
     8521 tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt ttccgaggtt
     8581 aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg tccgccaatt
     8641 tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt taaatcgcca
     8701 agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt gccgttaatt
     8761 tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc caggccgtcc
     8821 agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc gtcaatcgac
     8881 accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc tgaaatcagt
     8941 taagacattc tgttcagcac aatagcaggt ggcaaacgcc cttaccagtg aaggggtaag
     9001 aatgactatt ttttcactgg agaattaata aatcctcgct acaatagact gaatttcccc
     9061 tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata actacaaata
     9121 aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc attagcaatc
     9181 ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg
     9241 ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat ctggcttccg
     9301 gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc gcaattaaat
     9361 ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg gctggcaggt
     9421 tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat ccgctccatg
     9481 gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc gttgttgtgg
     9541 ggcgcgccgg gctatctgtt cttccaggtt gcccgtaacc aatgtgaagg tctggcaaaa
     9601 accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacattcc ggtgaactat
     9661 atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg tggcgtggct
     9721 actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa acgcgcccgc
     9781 tccatgcgcg atattcgtaa cgaaaaaggc accgcgaaac ccgatcctgc ggttatgaaa
     9841 cgactgattc aactcggttt accgattgcg ctggcactat tctttgaagt gacactgttt
     9901 gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg acaccagatt
     9961 gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc agcggtaact
    10021 atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac cgctgcgcgg
    10081 accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac ggtttcactc
    10141 cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat
    10201 ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat tggcagtggg
    10261 attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac agcttactgg
    10321 gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt tgaacctatg
    10381 gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc cattatgatg
    10441 atgttgcgta tgcggttcct gcaacgtctg ccgtcagtta tcattctgca acgagcatcc
    10501 cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac gcaggctgga
    10561 gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttgcac agagtttgcc
    10621 gactggcatc tgttatctca acgtgtatat caccggtaac ttcccctttc acattgatga
    10681 agtcaggtga gatttctttc gcactggttt gcaggcaaat cgctccctct ccgcgcaggc
    10741 gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt tttccgccat
    10801 tcagattgat gttaccaaca ataaaagtga caccgtcacc ttttaccgta ctgccgtcga
    10861 gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagatct gcgccatagc
    10921 caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca accggtgcgc
    10981 gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg atcactgttc
    11041 caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccga ccacccacat
    11101 tcacggacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc gtccccagac
    11161 gataaatgga tccattctca acaatggtgt cagagtcttc accaccgaga ttattcatgg
    11221 tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg ccgccctgat
    11281 taatctcaca attgctggct tccccacctg gatacaccaa caattcacca ccttcatcta
    11341 ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg agaatgattt
    11401 tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc aacaaaccgc
    11461 aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta accgtagcga
    11521 gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca atggcaccgt
    11581 cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc ccaccggttg
    11641 ccactttcaa ccagccagcc cccgtgacta cggtattttc ggctcttccg ccagcttcaa
    11701 caatcaactc gccttcagta agttgcgtcg cgccaatgac ctcgccggtc ttaacaattt
    11761 gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat tcctttgctg
    11821 catctgtgtg cctttattgc tacctaaatg taaaggctac ggaggattta tccacgacag
    11881 atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat tgggttgtat
    11941 cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa tatagccaat
    12001 ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc
    12061 accttcccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt gcgttcatag
    12121 ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag tccaattgaa
    12181 cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat ggtgggggtc
    12241 ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtcttct cctttttccc
    12301 tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt atgcgttatc
    12361 agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa tggcccattt
    12421 ggcgacgcaa tggctgagca gcttaaaccg cttgctgagt cgattaatca ggaacctggt
    12481 tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg gatctatctt
    12541 tttactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg acttaaaaat
    12601 ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact gagtcaaatc
    12661 aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc ttttcatccc
    12721 tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa cactaaattc
    12781 gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt gccgggccta
    12841 cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct atttctatct
    12901 tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa aactcaaaaa
    12961 tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg tatctcgaat
    13021 ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa gaaaccttgc
    13081 acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat caatttttac
    13141 gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa tcatgccagg
    13201 ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag gatttaccca
    13261 gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat gaagaagaag
//
LOCUS       DAJTNN010000108        13255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTNN010000108 DAJTNN010000000
VERSION     DAJTNN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.108"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..309
                     /locus_tag="OO150_002761"
     CDS             <1..309
                     /locus_tag="OO150_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5516843.1"
                     /translation="NSEYTYEDNGMYICPECAYEWNDTEPAQESDELIVKDANGNLLA
                     DGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMKLKSEFVKKN
                     "
     gene            469..912
                     /gene="yjdN"
                     /locus_tag="OO150_002762"
     CDS             469..912
                     /gene="yjdN"
                     /locus_tag="OO150_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5516844.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1045..1833
                     /gene="phnC"
                     /locus_tag="OO150_002763"
     CDS             1045..1833
                     /gene="phnC"
                     /locus_tag="OO150_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5516845.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            1858..2874
                     /gene="phnD"
                     /locus_tag="OO150_002764"
                     /pseudo
     CDS             1858..2874
                     /gene="phnD"
                     /locus_tag="OO150_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
     gene            3000..3779
                     /gene="phnE"
                     /locus_tag="OO150_002765"
     CDS             3000..3779
                     /gene="phnE"
                     /locus_tag="OO150_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR5516846.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3800..4525
                     /gene="phnF"
                     /locus_tag="OO150_002766"
     CDS             3800..4525
                     /gene="phnF"
                     /locus_tag="OO150_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR5516847.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4526..4978
                     /gene="phnG"
                     /locus_tag="OO150_002767"
     CDS             4526..4978
                     /gene="phnG"
                     /locus_tag="OO150_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR5516848.1"
                     /translation="MHADIATRQHWMSMLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQPRMGGTGERFFAGDATLTRAAVRLTDGPLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            4975..5559
                     /gene="phnH"
                     /locus_tag="OO150_002768"
     CDS             4975..5559
                     /gene="phnH"
                     /locus_tag="OO150_002768"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR5516849.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQSELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5559..6617
                     /gene="phnI"
                     /locus_tag="OO150_002769"
     CDS             5559..6617
                     /gene="phnI"
                     /locus_tag="OO150_002769"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR5516850.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            6610..7455
                     /gene="phnJ"
                     /locus_tag="OO150_002770"
     CDS             6610..7455
                     /gene="phnJ"
                     /locus_tag="OO150_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR5516851.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7452..8210
                     /gene="phnK"
                     /locus_tag="OO150_002771"
     CDS             7452..8210
                     /gene="phnK"
                     /locus_tag="OO150_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR5516852.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEGGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8321..9001
                     /gene="phnL"
                     /locus_tag="OO150_002772"
     CDS             8321..9001
                     /gene="phnL"
                     /locus_tag="OO150_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR5516853.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            8998..10134
                     /gene="phnM"
                     /locus_tag="OO150_002773"
     CDS             8998..10134
                     /gene="phnM"
                     /locus_tag="OO150_002773"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR5516854.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLNAVAIGDVR
                     DGGDQLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10134..10691
                     /gene="phnN"
                     /locus_tag="OO150_002774"
     CDS             10134..10691
                     /gene="phnN"
                     /locus_tag="OO150_002774"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR5516855.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10678..11127
                     /gene="phnO"
                     /locus_tag="OO150_002775"
     CDS             10678..11127
                     /gene="phnO"
                     /locus_tag="OO150_002775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR5516856.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYPSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLNVSSKL
                     LAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11129..11887
                     /gene="phnP"
                     /locus_tag="OO150_002776"
     CDS             11129..11887
                     /gene="phnP"
                     /locus_tag="OO150_002776"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR5516857.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWPPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12034..12363)
                     /gene="yjdP"
                     /locus_tag="OO150_002777"
     CDS             complement(12034..12363)
                     /gene="yjdP"
                     /locus_tag="OO150_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR5516858.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12432..12749)
                     /locus_tag="OO150_002778"
     CDS             complement(12432..12749)
                     /locus_tag="OO150_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR5516859.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13002..>13255
                     /locus_tag="OO150_002779"
     CDS             13002..>13255
                     /locus_tag="OO150_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516860.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPY"
BASE COUNT         2951 a         3969 c         3691 g         2644 t
ORIGIN      
        1 aactccgaat acacttacga agataacggc atgtacatct gcccggaatg tgcctacgaa
       61 tggaacgaca cagaacctgc acaggaaagc gacgagctga tcgttaaaga tgctaacggc
      121 aatctgctgg ctgacggcga cagcgttacc atcattaaag atctgaaggt gaaaggtagc
      181 tcttcgatgc tgaaaattgg caccaaagtg aaaaacatcc gcctggttga aggcgaccat
      241 aacatcgatt gcaaaatcga cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa
      301 aagaactgat tgtattgtga tcggtaagcc ggataaggcg ctcgcgccac atccggcaac
      361 gatgccgact gcctgatgcg acgctgaccg cgccttatca ggcttccctc tcatctgtat
      421 aaatttcgaa ctacacttaa ctggcttctc ttaactgagg tcaccatcat gccgttaagt
      481 ccctacctct cttttgccgg taactgttcc gacgcgattg cctattatca acgtacgttg
      541 ggcgcggaac tgctctataa aatcagcttc ggcgaaatgc caaaatcagc gcaggacagc
      601 gccgagaact gcccttccgg aatgcaattt cccgataccg ccatcgccca cgccaacgtg
      661 cgcattgccg gaagcgacat catgatgagc gatgctatac cgtcaggaaa agccagctac
      721 tccggcttta cgctggtgct cgattcgcaa caggtcgaag aaggaaaacg ctggtttgat
      781 aaccttgccg ctaacggaaa aattgaaatg gcctggcagg aaactttctg ggcgcatggc
      841 ttcggcaaag tcaccgataa atttggcgta ccgtggatga ttaatgtcgt caaacaacaa
      901 ccaacgcaat aacccgccgg gaggcccgcc ctcccgcact gtcatcgaat tcccgttaac
      961 tcttcatctg ttagtcactt ttaattaacc aaatcgtcac aataatccgc cacgatggag
     1021 ccactttttt agggaggctg catcatgcaa acgattatcc gtgtcgagaa gctcgccaaa
     1081 accttcaatc agcatcaagc gctgcatgcg gttgatctga acattcatca cagtgaaatg
     1141 gtggctctgc ttgggccgtc gggttccggc aaatccacac ttttacgtca cttaagcggt
     1201 ttgattaccg gcgataaatc cgccggcagc catatcgagc tgctgggccg cacagtccag
     1261 cgcgaaggcc gtctggcgcg cgatatccgc aaaagccgcg cccataccgg ctacatcttc
     1321 caacaattca acctggtgaa tcgcctgagc gtactggaga acgtgctgat tggcgcgctc
     1381 ggcagcacgc cgttctggcg cacctgtttt agctggttca cccgcgagca gaaacaacgc
     1441 gcgttacagg cgctgacccg cgttggcatg gtgcattttg cccatcaacg cgtttccacc
     1501 ctctccggcg gacagcagca gcgtgtggcg attgcccgcg cgctgatgca gcaggcgaag
     1561 gtgattctgg ccgatgaacc catcgcctcg ctggacccgg aatccgcccg catcgtgatg
     1621 gacaccctgc gcgacatcaa tcagaacgac ggcatcaccg tagtcgtcac gctgcatcag
     1681 gtggattacg ccctgcgcta ctgcgaacgc atcgtcgccc tgcgccaggg gcacgttttc
     1741 tacgacggca gcagccaaca gtttgataac gaacgttttg accatctcta ccgcagcatt
     1801 aatcgcatcg aagagaacgc gaaagctgcc tgacatcccc atcattgagg aaaacgaatg
     1861 aacgctaaga taattgcctc gctggccttc accagcatgt tcagcctcag caccctgtta
     1921 agcccggcac acgccgaaga gcaggaaaag gcgctgaatt tcggcattat ttcaacggaa
     1981 tcacagcaaa acctgaaacc gcaatagacg ccattcttac aggatatgga gaagaagctg
     2041 ggcgtgaagg tgaacgcctt ctttgcccca gactacgcag gcattatcca gggaatgcgc
     2101 ttcaataaag tggatatcgc ctggtacggc aacctgtcgg caatggaagc ggtggatcgc
     2161 gccaacggcc aggtcttcgc ccagacggtc gcggcggatg gatcgccagg ttactggagc
     2221 gtgttgatcg tcaacaaaga tagtccgatc aacaacctga acgatctgct ggcgaagcgg
     2281 aaagatctca ccttcggcaa tggcgatcct aactccacct ctggcttcct cgtccccggt
     2341 tactacgtct ttgccaaaaa caatatctcc gccagcgact ttaagcgcac cgtcaacgct
     2401 gggcatgaaa ccaacgcgct ggccgtcgcc aacaagcagg tggatgttgc caccaacaac
     2461 accgaaaacc tcgacaagct gaaaacctcc gcgccagaga agctgaaaga actgaaggtg
     2521 atctggaagt cgccgctgat cccaggcgat ccgatcgtct ggcgcaagaa tctctccgaa
     2581 accaccaaag acaagatcta cgacttcttt atgaactacg gaaaaacgcc ggaagagaaa
     2641 gcggtgctgg aacgcctggg ctgggccccg ttccgcgcct ccagcgacct gcaactggtg
     2701 ccgattcgcc agctcgcact gtttaaagag atgcagggcg taaaaagcaa taaaggactg
     2761 aatgagcagg acaagctggc gaaaaccacc gagattcagg cgcagctgga tgacttggac
     2821 cgcctgaaca acgcgttaag cgcgatgagt tcggtgagta aagcggtgca gtaatgtgct
     2881 cgatcggtcc cctctccctt gagggagagg gttagggtga gggggaaatg cgcacgctga
     2941 tgccctcatc ccggccctct cccacaggga gagggagaaa acattaaacg gagccaaaca
     3001 tgcaaaccat caccatcgcc ccacccaagc gtagctggtt ctcgcttctg agctgggccg
     3061 ttgttctcgc cgtgctggtc gtctcgtggc agggcgcgga aatggccccg cttacgctga
     3121 ttaaagacgg cggcaacatg gcaaccttcg ttgccgactt cttcccgccc gatttcagcc
     3181 agtggcagga ttacctcacc gaaatggccg tcacgctgca aatcgccgtc tggggcaccg
     3241 cgctggcagt ggttctctcc atccccttcg gtctgatgag cgccgaaaac ctggtgccgt
     3301 ggtgggttta ccagcccgtt cgccgcctga tggatgcctg ccgcgccatt aacgaaatgg
     3361 tcttcgccat gctgttcgtg gtcgccgtcg gtctcggccc gttcgctggc gtgctggcgc
     3421 tatttatcca caccaccggc gtgctctcca agctgctttc cgaagcggtg gaagcgatcg
     3481 aacccggccc ggtagaaggc attcgcgcca ccggcgccaa caagctggaa gagatccttt
     3541 acggcgtgct gccacaggta atgccgctgc tgatctccta ctccctctat cgcttcgaat
     3601 ccaacgtccg ctcggcgacc gtcgtcggca tggtcggtgc aggcgggatc ggcgtcaccc
     3661 tgtgggaagc gattcgcggt tttcagttcc aacaaacctg cgccctgatg gtgcttatca
     3721 tcgtcacggt cagcctgctg gatttcctct ctcaacggtt gcgtaagcac tttatctgat
     3781 aagcgaggca ttgatatcta tgcacttgtc tacacatccg accagctacc caacacgcta
     3841 tcaggagata gccgcaaaac ttgagcagga gcttcgtcaa cactatcgct gcggcgacta
     3901 tcttcccgcc gagcagcagc tggcggcgcg ctttgaggta aatcgccaca ccctgcgccg
     3961 cgccatcgac caactggtgg aaaaaggctg ggtacagcgc cgtcagggcg tcggcgtgct
     4021 ggtgctgatg cgcccgttcg attacccgct caacgcccag gcgcgtttta gccagaacct
     4081 gctggatcag ggcagccatc ccaccagcga aaaactgctg tcggtgctgc gcccggcatc
     4141 tggtcacgtc gctgacgcgc tggggataac cgagggggag aacgtcatcc acctgcgcac
     4201 cctgcgccgg gtcaacggca tcgcgctctg tttaatcgac cactactttg cggacctcac
     4261 cctttggccg acgctgcaac gcttcgacag cggctcgctg cacgattttc tgcgcgagca
     4321 aaccgggatt gcgctgcgcc gcagccagac gcggatcagc gcccgccgcg cccaggccaa
     4381 agagtgccag cgtcttgaaa tcccgaatat gtcgccgctg ctgtgcgtgc gcacccttaa
     4441 ccaccgtgac ggtgaaagca gcccggcgga gtactccgtc agcctgacgc gcgccgacat
     4501 gattgaattc actatggagc actgaatgca cgcagatatc gcgacccgcc agcactggat
     4561 gtccatgctg gcgcacagcc aaccggctga actggcagcc cgcctgaacg cgctgaacat
     4621 caccgccaac tatgaagtga tccgcaccgc cgaaaccggc ctggtacaga ttcagccgcg
     4681 aatgggcggt accggcgaac gcttttttgc cggcgacgcc acgctgaccc gcgccgccgt
     4741 acgcctgact gacggccccc tcggctacag ctgggtgctg gggcgcgaca aacagcacgc
     4801 cgaacgctgc gcgctgattg acgcgttgat gcagcaatct cgccattttc aaaacttatc
     4861 agaaaccctt attgccccac tggacgctga ccgtatggca cgcattgccg cacgccaggc
     4921 cgaagtgaac gccagccggg tcgacttctt tacgatggtt cgcggagaca acgcatgacc
     4981 ctggaaaccg cttttatgct tcctgtgcag gatgcccagc acagttttcg tcgcctgtta
     5041 aaggccatga gcgagccggg cgtgattgtc gccctgcatc agctcaaacg cggctggcag
     5101 ccgctgaata tcgccaccac cagcgtgctg ctgacgctgg ctgataacga cacgccggtg
     5161 tggctttccg cgccattaag taacgatatc gtcagccaga gcctgcgttt tcataccaac
     5221 gcgccgctgg tcaatcagtc ggaactggcg accttcgcgg taacggatga ggcgatttcc
     5281 agcgaacagc tcaacgctct ttccagcggc accgctgttg cgccggaagc gggcgcgacg
     5341 ctgattttac aggtcgtcag cctgagcggc gggcgcatgc tgcgccttac tggcgcgggt
     5401 attgccgaag aacgaatgat cgctccgcag ctgccggagt gcattctgca cgaactcacc
     5461 gagcgcccgc acccgttccc gctcggcatc gacctgatcc tgacctgcgg cgagcggctg
     5521 ctggctatcc cgcgaaccac tcatgtggag gtgtgctgat gtacgttgcc gtgaaagggg
     5581 gcgagaagga gatcgacgcc gcccacgccc tgcaagagag ccgacgccgg ggcaataccg
     5641 atttgcctga actgagcgtc gcccagattg aacagcagct taatcttgca gtagatcgcg
     5701 tgatgaccga aggcggcatt gccgaccgcg aactggcggc gctggcgctg aaacaggcca
     5761 gcggcgataa cgtcgaagcg attttcctgc tacgcgccta ccgcaccacg ctggcgaagc
     5821 tggcggtaag cgagccactc gacaccacgg agatgcgtct cgaacggcgt atttcagcgg
     5881 tttataaaga tattcccggc ggtcagttgc ttggcccaac ctacgactac acccatcgcc
     5941 tgctcgattt caccctgctg gcaaacggcg aagcgccgac gctgaccacc gcagacagcg
     6001 aacaatcccc gtcgccgcac gttttcagcc tgctggcgcg tcaggggctg gcgaagtttg
     6061 aagaggatag cggcgcacag ccggatgaca tcacccgcac tccgccagtt tacccctgtt
     6121 cacgttcttc ccgtttgcag cagttgatgc gcggcgacga aggctatttg ctggcgctgg
     6181 cctactctac ccagcgcggt tacggacgca atcacccgtt cgcgggcgaa atccgcagtg
     6241 gttacatcga cgtgtcgatt atgccggaag agctgggatt tgcggtgaac gtcggcgaac
     6301 tgctgatgac cgagtgtgaa atggtcaacg gttttattga cccgccggat gagccgccgc
     6361 acttcacgcg cggctacggg ctggtgttcg gcatgagcga gcgcaaagcg atggcgctgg
     6421 ttgaccgtgc gttgcaggct ccggagtacg gcgagcacgc gacaggcccg gcgcaggatg
     6481 aagagttcgt gctggcacat gccgacaacg tcgaagccgc aggctttgtc tcgcacctca
     6541 aactccccca ctacgtcgat ttccaggccg aactggagct actcaaacgt ctgcaacagg
     6601 agcagaacca tggctaatct gagcggctac aactttgcct acctcgacga gcagaccaaa
     6661 cgcatgatcc gccgcgccat cttaaaagcg gtggcgatcc ccggttatca ggtgccgttt
     6721 ggcgggcgcg agatgccgat gccctacggc tggggaaccg gcggcattca gctcaccgcc
     6781 agcgtgattg gcgaaagcga cgtgctgaag gtgattgacc agggcgcaga cgacaccacc
     6841 aacgccgtgt cgattcgcaa cttctttaag cgcgtgaccg gggtaaacac cactgaacgt
     6901 acggacgatg cgacgcttat ccagacgcgc catcgcatcc ccgaaacgcc gctgaccgaa
     6961 gatcagatca ttatcttcca ggtgccgatc cccgaaccgc tgcgctttat cgagccgcgc
     7021 gaaacggaaa cccgcaccat gcacgcgctg gaagagtacg gcgtgatgca ggtgaaactg
     7081 tatgaagata tcgcccgctt cggtcatatc gccactacct acgcctatcc ggtgaaggtg
     7141 aacgggcgct acgtgatgga cccgtcgccg atcccgaaat tcgataaccc aaaaatggac
     7201 atgatgcccg ccctgcaact gttcggcgcg gggcgcgaga agcgcatcta tgcggtgccg
     7261 ccgtttaccc gcgtggaaag tctcgatttc gacgatcacc cgttcaccgt tcagcagtgg
     7321 gatgagccat gcgccatctg cggatcgacc cacagctatc ttgatgaagt ggtgctggat
     7381 gacgccggaa accgcatgtt tgtctgctcc gataccgatt attgccgcca acagagcgag
     7441 gcaaaaaacc aatgaatcaa ccgttacttt cggtcaataa cctgacccac ctttacgcgc
     7501 cgggcaaagg ctttagcgat gtctcttttg atttatggcc gggggaagtg ctgggcattg
     7561 tcggggagtc cggctccgga aagaccacgc tgctgaaatc aatctccgcg cgcctgacgc
     7621 cgcagcaggg ggagattcac tatgagagcc gttcgctgta cgcaatgagc gaggccgacc
     7681 gccgtcgcct gctgcgcacc gaagggggcg tagtgcatca gcatccgctc gacggcctgc
     7741 gccgccaggt gtcggcaggc ggcaatatcg gcgagcggct gatggcgacc ggggcacgtc
     7801 attacggcga tattcgtgcc accgcgcaga agtggctgga agaggtggaa attcccgcca
     7861 accggatcga cgacctgccg accacctttt ccggcggcat gcagcaacgt ttgcagatcg
     7921 cccgcaacct ggtgacgcat ccgaagctgg tgtttatgga cgagccgacc ggcggactgg
     7981 atgtgtcggt acaggcacgc ctgctcgacc tgctgcgcgg cctggtggtg gagctgaacc
     8041 tcgcggtagt gattgtcacc cacgatttag gcgtcgcccg cctgctggcg gaccgtttgc
     8101 tggtgatgaa gcaggggcaa gtggtggaga gtgggttaac cgaccgcgtg ctcgacgacc
     8161 cgcaccatcc gtatacacaa ctgctggtgt catcggtttt gcagaattga gccggtgccg
     8221 gatgcggcgt aaacgcctta tccggcctac aaatgcgctc cccgtaggtc ggataagacg
     8281 cgtcagcgtc gcatccgaca cccgaaccac gaggcgaaaa atgattaacg tacaaaacgt
     8341 cagtaaaacc ttcatcctgc atcagcaaaa cggcgtacgc ctgcccgtct tacaacgtgc
     8401 ctcgctcacc gtcaacgcag gcgaatgcgt ggtgctccac ggtcactccg gcagcggcaa
     8461 atcaaccctg ctgcgctcgc tgtacgccaa ctatctaccc gacgaaggtc aaatccagat
     8521 caaacacggt gacgagtggg tagacctggt caccgcgcca gcgcgcaaag tggtggaaat
     8581 ccgcaaaacc acagtcggct gggtgagcca gtttctgcgc gtcatcccgc gtatctcagc
     8641 actggaagtg gtgatgcagc cgctgctcga taccggcgtt ccgcgtgaag cctgcgccgc
     8701 taaagccgcg cgtcttctca cccgcctgaa cgtgccggaa cgcctgtggc acctggcacc
     8761 atcgaccttc tccggcggcg aacagcagcg cgtcaacatc gcccgcggct ttatcgtcga
     8821 ctaccccatt ctgctgctcg acgaacctac cgcctcgctg gacgccaaaa acagcgccgc
     8881 ggtggtggaa ctgattcacg aagccaaagc ccgtggcgca gccatcgtcg gcatcttcca
     8941 tgacgaagct gtacgtaatg acgtcgccga ccgcctgcac ccaatgggag cctcttcatg
     9001 attatcaata acgttaagct ggtgctggaa aacgaggtgg tgcacggttc gctggaggtg
     9061 caggatggcg aaatccgcgc ctttgccgaa agccagagcc gcctgccaga agcgatggac
     9121 ggcgaaggtg gctggctact acccggcctg attgagctgc ataccgacaa cctcgataaa
     9181 ttcttcaccc cgcgcccgaa agtcgactgg cccgcccatt cggcgatgag cagccacgac
     9241 gcgctgatgg tggcaagcgg catcaccacc gtgctgaacg cggtggcgat tggcgacgtg
     9301 cgcgacggcg gcgatcagct ggagaatctg gagaagatga tcaacaccat cgaagagacg
     9361 cagaaacgcg gcgtcaaccg cgccgagcac cgtctgcacc tgcgctgcga gctgccgcat
     9421 cacaccacac tgccgctgtt tgaaaaactg gttcagcgcg aaccggtgac gctggtgtcg
     9481 ctaatggacc actcaccggg ccagcgccag ttcgctaacc gcgagaagta tcgcgaatat
     9541 tatcagggca aatactccct caccgatgcg cagatgcagc agtacgaaga agagcaattg
     9601 gcgctcgccg cacgctggtc gcagccgaat cgcgaatcca tcgccgccct gtgccgcgcg
     9661 cgacaaattg cgctcgccag ccacgatgac gccacgcacg cccacgttgc cgaatcccac
     9721 caacttggca gcgtgatcgc cgaatttacc accacgttcg aagcggcgga agcctcgcgt
     9781 aagcatggca tgaacgtgct gatgggtgcg ccaaatatcg tgcgtggcgg ctcgcactcc
     9841 ggcaatgtgg cggccagtga actggcgcag cttggtctgc tggatatcct ctcttccgac
     9901 tactaccccg ccagcctgct ggatgcggcg ttccgcgtcg ccgatgacga gagcaaccgc
     9961 tttacgctac cgcaggcggt gaggctggtg accaaaaatc cggcgcaggc gctgaatctt
    10021 caggatcgcg gggtgattgg cgagggtaaa cgcgctgacc tggtgctggc gcatcgccag
    10081 ggcaatcaca ttcatatcga ccacgtctgg cgtcagggta aaagggtgtt ctgatgatgg
    10141 gaaaactgat ttggttaatg gggccgtccg gctccgggaa agacagcctg ctggcggaac
    10201 tccgcctgcg ggaacaaacg cagttactgg tggcgcatcg ctacatcacg cgcgacgcca
    10261 gcgccggaag tgaaaaccat atcgccctga gtgagcggga gttttttacc cgcgcggggc
    10321 aaaacctgct ggccttaagc tggcacgcta acggtctgta ttatggcgtc ggcatcgaga
    10381 ttgacctctg gctgcacgct ggattcgacg tagtcgtcaa cggttcacgc gcgcatttgc
    10441 cgcaggcacg ggcgcgctat caatcggcgc tgctgcccgt ctgtttacag gtttcgccgg
    10501 agatcctgcg ccagcgcctg aaaaaccgtg gccgtgaaaa caccagtgaa attaacgccc
    10561 ggctggcgcg cgccgcccgc tatactccac aggattgcca tacgctcaac aatgacggca
    10621 gcctgcgcca gtcggtcgac acactgctga cgctgattca tcagaaggag aaacaccatg
    10681 cctgcttgtg agcttcgccc ggccacgcag tacgataccg acgcggttta cgcgctgatt
    10741 tgtgagctaa aacaggcgga gtttgaccac cacgcgtttc gcgtgggctt taacgccaac
    10801 ctgcgcgacc cgagcatgcg ctacccgagc atgcgctacc atctggcgct gctggatggc
    10861 gaagttgtcg gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt caactggatc
    10921 ggcgaaattc aggaactggt ggtgatgccg caggcgtgcg gtctgaacgt cagcagtaag
    10981 ttactggcgt gggcagaaga agaagcccgc caggccgggg ccgaaatgac cgaactttcc
    11041 accaacgtga agcgccacga cgcgcaccgt ttttacctgc gtgaaggcta cgagcagagc
    11101 cacttccgct tcaccaaggc gctgtaacat gagcctgacc ctcacgctca ccggcaccgg
    11161 cggcgcacag ggcgttccgg catggggctg cgagtgcgta gcctgcgcca gagcgcggcg
    11221 ctcaccgcag tatcgccgcc agccatgcag cggcgtagtg aagtttaacg acgcaatcac
    11281 cctgatcgac gccgggctgc acgatctcgc cgatcgctgg ccgcccggat cgttccagca
    11341 gtttttgctg acgcactacc atatggatca cgtgcagggg ctattcccgc tgcgctgggg
    11401 cgtgggcgat ccgatcccgg tttacggccc gccggatgaa cagggctgcg acgatctgtt
    11461 taaacatccg ggcctgcttg atttcagcca cacggtggaa ccgtttgtgg tgtttgattt
    11521 gcaggggtta caggtcacgc ccctgccgct caaccactca aaactgacct tcggttatct
    11581 gctggaaacg gcacacagcc gggtggcgtg gctgtctgac accgcaggct tgccggaaaa
    11641 aacgctgaaa tttttacgca ataatcagcc gcaggtaatg gtgattgatt gcagccaccc
    11701 gccgcgcgag gatgcgccac gtagtcactg tgatttaaac accgtgctgg cgctgaatca
    11761 ggttatccgc tcgccacggg tgattctgac ccatatcagc caccagtttg atgcgtggct
    11821 gatggaaaac gcactgccgt cggggtttga ggcgggattt gatgggatgg agattggggt
    11881 ggcgtgatga gagggaatgt gcgcgctggc cccctcaccc taaccctctc cccagagggg
    11941 cgaggggacc gattgtgctc gatattgaat attgcgctgg ttttctccct ctccccattg
    12001 gggtgagggg cgatgcctgc tccataccca acctcatcgc ccatactcat cttccattct
    12061 ccgctcttca tcttctagct gccgacgctc ctgatcaagc tggcgctggc gatcgtccag
    12121 ctgcctgcgg cgatcttcaa actggcggcg gcggtcgtca tattgtctgc gccgatcgtc
    12181 gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc cacggtcagg
    12241 gttataagcg tcgttaatcg cctgctgaat attgccaatg gtgtcgtcga taatatcggc
    12301 ctgggccgga acggtggaca gcgtgagcag ggtgaataaa agaaatagcg gaaagcgttt
    12361 cattagccaa cctcaaaaag aaactctatc cacattaatc attactcatc catgcaagta
    12421 gtggatgaat ctcaatttct ccgctgctct attgccgtaa tcgcctccac gcgttgttga
    12481 tgacgaccgc cttcgtactg tgcgcccagc cacgcatcca caatcatttt tgccagttcg
    12541 aggccaacca ctcgtgaacc aaaagccagt gcaacttcac tggcataatg cggataatca
    12601 gtgcgctctg acgacctggt gtctttatca atcacctcac tcattgacca tgaataaaag
    12661 gctggcacag caacgcagaa tcctgcggtg ccagcgacat gaccgcccat tgctccggta
    12721 cgttttttaa aaagaaacac cggttgcatt gataaattgt gctcagtatt tgccgaagta
    12781 caatatatat aataaatata taaaacacct catattattt acagagataa taaattcgcg
    12841 taattttctc tcttcagcac aaatgcctaa taaaatggat cgttcagctc acacttccac
    12901 ttattaaagt cgcaaattcc caattatgat aaccccagct ttaaatgacg tattttgtat
    12961 ggattacata tttacgacat aaatgataaa tggagattta tatgaataac tctcggttat
    13021 tccgtttgag cagaattgtt attgcgttaa ctgccgccag cggcatgatg gtaaataccg
    13081 ctaacgcgaa agaggaagcg aaagccgcca ctcaatatac ccaacaggtt aatcagaatt
    13141 acgccaaatc attaccgttt agcgatcgtc aggattttga cgacgctcag cgtggattta
    13201 tcgctccgct gctggatgaa ggtattctgc gcgatgctaa tggcaaacca tatta
//
LOCUS       DAJTNN010000109        13208 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTNN010000109 DAJTNN010000000
VERSION     DAJTNN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.109"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(185..448)
                     /gene="rpsT"
                     /locus_tag="OO150_002780"
     CDS             complement(185..448)
                     /gene="rpsT"
                     /locus_tag="OO150_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5516861.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            551..769
                     /locus_tag="OO150_002781"
     CDS             551..769
                     /locus_tag="OO150_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5516862.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            777..1718
                     /gene="ribF"
                     /locus_tag="OO150_002782"
     CDS             777..1718
                     /gene="ribF"
                     /locus_tag="OO150_002782"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5516863.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYVVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1761..4577
                     /gene="ileS"
                     /locus_tag="OO150_002783"
     CDS             1761..4577
                     /gene="ileS"
                     /locus_tag="OO150_002783"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5516864.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4577..5071
                     /gene="lspA"
                     /locus_tag="OO150_002784"
     CDS             4577..5071
                     /gene="lspA"
                     /locus_tag="OO150_002784"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5516865.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5140..5589
                     /gene="fkpB"
                     /locus_tag="OO150_002785"
     CDS             5140..5589
                     /gene="fkpB"
                     /locus_tag="OO150_002785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5516866.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5591..6541
                     /gene="ispH"
                     /locus_tag="OO150_002786"
                     /gene_synonym="lytB"
     CDS             5591..6541
                     /gene="ispH"
                     /locus_tag="OO150_002786"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5516867.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6607..7521
                     /gene="rihC"
                     /locus_tag="OO150_002787"
     CDS             6607..7521
                     /gene="rihC"
                     /locus_tag="OO150_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5516868.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7688..8509
                     /gene="dapB"
                     /locus_tag="OO150_002788"
     CDS             7688..8509
                     /gene="dapB"
                     /locus_tag="OO150_002788"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5516869.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            8964..10112
                     /gene="carA"
                     /locus_tag="OO150_002789"
     CDS             8964..10112
                     /gene="carA"
                     /locus_tag="OO150_002789"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5516870.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            10130..>13208
                     /gene="carB"
                     /locus_tag="OO150_002790"
     CDS             10130..>13208
                     /gene="carB"
                     /locus_tag="OO150_002790"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5516871.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDS"
BASE COUNT         3056 a         3341 c         3741 g         3070 t
ORIGIN      
        1 agccgatggt tctacgattc ttaagccacg aagagttcag aaagtgtaac ggcgtgtctg
       61 caaaagccag catcttttga ctatctggca accggtagtg tgttctctca cgcatcataa
      121 aagcagcagg cataaaaaaa cccgcttgcg cgggcttttt cacaaagctt cagcaaattg
      181 gcgattaagc cagtttgttg atctgtgcag tcaggttagc cttatgacgt gcagctttgt
      241 ttttgtggat cagaccttta gcagcctgac ggtccacgat cggttgcatt tcgttaaatg
      301 ctttctgtgc agcagctttg tcgccagctt cgatagctgc gtatactttc ttgatgaaag
      361 tacgcatcat agagcgacgg cttgcgttgt gcttacgagc cttttcagac tgaatggcgc
      421 gcttcttagc tgatttgata ttagccaagg tccaactccc aaatgtgttc tatatggaca
      481 attcaaaggc cgaggaatat gcccttttag ccttcttttg tcaatggatt tgtgcaaata
      541 agcgccgtta atgtgccggc actcgttacg tagtgatggc gcaggattct accagcttgc
      601 ggggtgtgaa tacagctttt ccgcgataaa aattgcagca ggcggtcact ttcttcccgt
      661 gatttgcgcc atggcaatga aaagccactt ctttctgatt tcggtactca atcgccggtt
      721 aaccttgacc gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga
      781 agctgatacg cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta
      841 ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag
      901 aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac
      961 tgtttgctac cgataaagcc ccggcaagac tgacccggct gcgggaaaaa ctgcgttacc
     1021 ttgcagagtg tggcgttgat tacgtgctgt gcgtgcgttt cgacaggcgt ttcgcggcgt
     1081 taaccgcgca aaatttcatc agcgatcttc tggtgaagca tttgcgcgta aaatttcttg
     1141 ccgtagggga tgatttccgc tttggcgctg gtcgtgaagg cgatttcttg ttattacaga
     1201 aagctggcat ggaatacggc ttcgatatca ctagtacgca aactttttgc gaaggtggcg
     1261 tgcgtattag cagcaccgct gtgcgtcagg cgctggcgga tgacaatctg gctctggcag
     1321 aaagtttact ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag
     1381 ggcgcactat aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga
     1441 aaggggttta tgtggtagaa gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa
     1501 acatcggaac acgcccaacg gttgccggta ttcgccagca gctggaagtg catttgttag
     1561 atgttgcaat ggacctttac ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca
     1621 atgagcagcg atttgcgtcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa
     1681 ccgcccgcga attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga
     1741 aacacggaac cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag
     1801 ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg
     1861 atgatgatct gtacggcatc atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc
     1921 atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc
     1981 tgaaagacat tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg
     2041 gctgggactg ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg
     2101 gtgagaaatt caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg
     2161 ttgacggtca acgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt
     2221 acctgaccat ggacttcaaa actgaagcca acatcatccg cgcgctgggc aaaatcatcg
     2281 gcaacggtca cctgcacaaa ggcgcgaagc cggtacactg gtgcgttgac tgccgttctg
     2341 cgctggcgga agcggaagtt gagtattacg acaaaacttc tccgtccatc gacgttgctt
     2401 ttcaggcggt cgatcaggat gcactgaaag caaaatttgc cgtaagcaac gttaacggcc
     2461 caatctcgct ggtgatctgg accactacgc cgtggactct gcctgcgaac cgcgcaatct
     2521 ctattgcacc tgatttcgac tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg
     2581 cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac accattctcg
     2641 gcacggtaaa aggtgcggag cttgagttgc tgcgctttac ccatccgttt atgggcttcg
     2701 acgttccggc aatcctcggc gatcacgtta ccctggatgc cggtaccggt gccgttcaca
     2761 ccgcgcctgg ccacggcccg gacgactatg tgatcggtca gaaatacggc ctggaaaccg
     2821 ctaacccggt tggcccggac ggcacttatc tgccgggcac ttatccgacg ctggatggcg
     2881 tgaacgtctt caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc
     2941 tgcacgttga gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca
     3001 tcttccgcgc gacgccgcag tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt
     3061 cactgaaaga gatcaaaggc gtgcagtgga tcccggactg gggccaggcg cgtatcgagt
     3121 cgatggttgc taaccgtcct gactggtgta tctcccgtca gcgcacctgg ggcgtaccga
     3181 tgtcactgtt cgtgcacaaa gacacggaag agctgcatcc gcgtactctc gaactaatgg
     3241 aagaagtggc aaaacgcgtt gaagttgacg gcatccaggc gtggtgggat cttgatgcga
     3301 aagagatcct cggcgatgaa gctgatcagt acgtgaaagt gccggacaca ttggatgtat
     3361 ggtttgactc cggatctacc cactcttctg ttgttgacgt gcgtccggaa tttgccggtc
     3421 acgcagcgga catgtatctg gaaggttctg accagcaccg tggttggttc atgtcttccc
     3481 taatgatctc caccgcgatg aaaggcaaag cgccgtatcg tcaggtactg acccacggct
     3541 ttaccgtgga tggtcagggc cgcaagatgt ctaaatccat cggcaatacc gtttcgccgc
     3601 aggatgtgat gaacaaactg ggcgcggata ttctgcgtct gtgggtggca tcaaccgact
     3661 acaccggtga aatggccgtt tctgacgaga tcctgaaacg tgctgccgat agctatcgtc
     3721 gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccagcaaaag
     3781 atatggtgaa accggaagag atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag
     3841 cggcacagga agacatcctc aaggcgtacg aagcatacga tttccacgaa gtggtacagc
     3901 gtctgatgcg cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc
     3961 gtcagtacac cgccaaagcg gacagtgtgg cgcgtcgtag ctgccagact gcgctgtatc
     4021 acatcgcaga agcgctggtg cgctggatgg caccaatcct ctccttcacc gctgatgaag
     4081 tgtggggcta cctgccgggc gaacgtgaaa aatacgtctt caccggtgag tggtacgaag
     4141 gcctgtttgg cctggcagac agcgaagcga tgaacgatgc gttctgggac gagctgttga
     4201 aagtgcgtgg cgaagtgaac aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg
     4261 gctcgctgga agcggcagta accttgtatg cagaaccgga actggcggcg aaactgaccg
     4321 cgctgggcga tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactatc
     4381 acgacgcacc tgctgatgct cagcagagcg aagtactcaa agggctgaaa gtcgcgttga
     4441 gtaaagccga aggtgagaag tgcccacgct gctggcacta cacccaggat gtcggcaagg
     4501 tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa
     4561 aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct
     4621 ggtggtagtc gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc
     4681 tctgggggat acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg
     4741 cgcggcgttt agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat
     4801 tgcgattggt attagcgtga tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa
     4861 gctaaacaat atcgcttacg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg
     4921 cctgtggcac ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc
     4981 caccttcaac cttgccgata ctgccatctg tgtcggtgcg gcactgattg tgctggaagg
     5041 ttttttgcct tctagagcga aaaaacaata ataaaccctg ccggataagg cgtttacgcc
     5101 gcatccggca aaaatcctca aatataagag caaacctgca tgtctgaatc tgtacagagc
     5161 aatagcgccg tcctggtgca cttcacgcta aaactcgacg atggcaccac cgccgagtct
     5221 acccgcaaca acggtaaacc ggcgctgttc cgcctgggtg atgcttctct ttctgaaggg
     5281 ctggagcaac acctgttggg gctgaaagtg ggcgataaaa ccaccttctc gttggagcca
     5341 gatgcggcgt ttggcgtgcc gtcaccggac ctgattcagt acttctcccg ccgtgaattt
     5401 atggatgcag gcgagccaga aattggcgca atcatgcttt ttaccgcaat ggatggcagt
     5461 gagatgcctg gcgtgatccg cgaaattaac ggcgactcca ttaccgttga tttcaaccat
     5521 cctctggccg ggcagaccgt tcattttgat attgaagtgc tggaaatcga tccggcattg
     5581 gaggcgtaac atgcagatcc tgttggccaa cccgcgtggt ttttgtgccg gggtagaccg
     5641 cgctatcagc attgttgaaa acgcgctggc catttacggc gcaccgatat atgtccgtca
     5701 cgaagtggta cataaccgct atgtggtcga tagcttgcgt gagcgtgggg ctatctttat
     5761 tgagcagatt agcgaagtac cggacggcgc gatcctgatt ttctccgcac acggtgtttc
     5821 tcaggcggta cgtaacgaag caaaaagtcg cgatttgacg gtatttgacg cgacctgtcc
     5881 gctggtgacc aaagtgcata tggaagtcgc ccgcgccagt cgccgtggcg aagaatctat
     5941 tctcatcggt cacgccgggc acccggaagt ggaagggaca atgggccagt acagtaaccc
     6001 ggaaggggga atgtatctgg tcgaatcgcc ggacgatgtg tggaaactga cggtcaaaaa
     6061 cgaagagaag ctctccttta tgacccagac cacgctgtcg gtggatgaca cgtctgatgt
     6121 gatcgacgcg ctgcgtaaac gcttcccgaa aattgtcggt ccgcgcaaag atgacatctg
     6181 ctacgccacg actaaccgtc aggaagcggt acgcgccctg gcagaacagg cggaagttgt
     6241 gttggtggtc ggttcgaaaa actcctccaa ctccaaccgt ctggcggagc tggcccagcg
     6301 tatgggcaaa cgcgcgtttt tgattgacga tgcgaaagac atccaggaag agtgggtgaa
     6361 agaggttaaa tgcgtcggcg tgactgcggg cgcatcggct ccggatattc tggtgcagaa
     6421 tgtggtggca cgtttgcagc agctgggcgg tggtgaagcc attccgctgg aaggccgtga
     6481 agaaaacatt gttttcgaag tgccgaaaga gctgcgtgtc gatattcgtg aagtcgatta
     6541 agtcattagc agcctaagtt atgcgaaaat gccggtcttg ttaccggcat tttttatgga
     6601 gaaaacatgc atttacctat cttcctcgat actgaccccg gcattgacga tgccgtcgcc
     6661 attgccgccg cgatttttgc acccgaactc gacctgcaac tgatgaccac cgtcgcgggt
     6721 aatgtctcgg ttgagaaaac cacccgcaac gccctgcaac tgctgcattt ctggaatgcg
     6781 gagattccgc tcgcccaggg agccgctgtg ccgctggtac gcgcaccgcg tgatgcggca
     6841 tctgtgcacg gcgaatcggg aatggctggc tacgactttg ttgagcacaa ccgaaagccg
     6901 ctcgggatac cagcgtttct ggcgattcgg gatgccctga tgcgtgcacc agagcccgtt
     6961 accctggtgg ccatcggccc gttaaccaat attgcgctgt tactttcaca atgcccggaa
     7021 tgtaaaccgt atattcgtcg tctggtgatc atgggcggtt ctgctggtcg cggcaactgc
     7081 acaccaaacg ccgagtttaa tatcgctgcc gatccagaag cggctgcctg tgtcttccgt
     7141 agcggcatcg aaatcgtcat gagcggtctg gatgtcacca atcaggcaat attaacccct
     7201 gactatctcg ctactttgcc ggagttgaat cgcaccggaa aaatgctcca cgccctgttt
     7261 agccactacc gtagcggcag tatgcaaagt ggactgcgta tgcacgatct ttgcgctatt
     7321 gcctggctgg tgcgcccgga attgttcact ctcaaaccgt gttttgtggc agtggaaact
     7381 cagggcgaat ttacctcagg cacgacggtg gttgatatcg acggttgcct gggcaaacca
     7441 gccaatgtaa aggtggcatt ggatctggat gtgaaaggct tccagcagtg ggtggctgag
     7501 gtgctggctc tggcgtcgta acctgtcaca tgttatcggc atgcagtcat tcatcgactc
     7561 atgcctttca ctgatatccc tccctgttta tcattaattt ctaattatca gcgtttttgg
     7621 ctggcggcgt agcgatgcgc tggttactct gaaaacggtc tatgcaaatt aacaaaagag
     7681 aatagctatg catgatgcaa acatccgcgt tgccatcgcg ggagccgggg ggcgtatggg
     7741 ccgccagttg attcaggcgg cgctggcatt agaaggcgtg cagctgggcg cggcgctgga
     7801 gcgcgaagga tcttctttac tgggcagcga cgccggtgag ctggctggtg cagggaaaac
     7861 aggcgttacc gtgcaaagca gcctcgatgc ggtaaaagat gattttgatg tgtttataga
     7921 ttttacccgt ccggaaggta cgctgaacca tctcgctttt tgtcgccagc atggcaaagg
     7981 gatggtgatc ggtactacgg ggtttgacga agccggtaaa caggcgattc gtgacgctgc
     8041 cgccgatatt gcgattgtct ttgccgccaa ttttagcgtt ggcgttaacg tcatgcttaa
     8101 gctgctggag aaagcagcca aagtgatggg tgactacacc gatatcgaaa ttattgaagc
     8161 acatcataga cataaagttg atgcgccgtc aggcaccgca ctggcaatgg gagaggcgat
     8221 cgcccacgcc cttgataaag atctgaaaga ttgcgcggtc tacagtcgtg aaagccacac
     8281 cggtgaacgt gtgcctggca ccattggttt tgccaccgtg cgtgcaggtg acatcgttgg
     8341 cgaacatacc gcgatgtttg ccgatattgg cgagcgtctg gagatcaccc ataaggcgtc
     8401 cagccgcatg acatttgcta acggcgcggt aagatcggct ttgtggttga gtggtaagga
     8461 aagtggtctt tttgatatgc gagatgtgct tgatctcaat agtttataac cacaaaatat
     8521 ttgttatggt gcaaaaataa cacatttaat ttattgatta taaagggctt taattttggc
     8581 ccttttattt ttagtgttat gtttttaaat tgtctataag tgccaaaaat tacatgtttt
     8641 gtcttctgtt tttgttgttt taatgtaaaa tttgaccatt tggtccactt ttccctgctc
     8701 gcttttattt catgcaatct tcttgctgtg caagcgtttt ccagaacagg ttatatgatc
     8761 tttttgtcgc ttaatgcctg taaaacatgc atgagccaca aaataatata aaaaatcccg
     8821 ccattaagtt gacttttagc gcccatatct ccagaatgcc gccgtttgcc agaaattcgt
     8881 cggtaagcag atttgcattg atttacgtca tcattgtgaa ttaatatgca aataaagtga
     8941 gtgaatattc tctggagggt gttttgatta agtcagcgct attggttctg gaagacggaa
     9001 cccagtttca cggtcgggcc ataggggcaa caggttcggc ggttggggaa gtcgttttca
     9061 atacttcaat gaccggttat caagaaatcc tcactgatcc ttcctattct cgtcaaatcg
     9121 ttactcttac ttatccccat attggcaatg tcggcactaa tgacgccgat gaagaatctt
     9181 ctcaggtaca tgcacaaggt ctggtgattc gcgacctgcc gctgattgcc agcaacttcc
     9241 gtaataccga agacctctct tcttacctga agcgccataa catcgtggcg attgccgata
     9301 tcgatacccg taagctgacg cgtttactgc gcgagaaagg cgcacagaat ggctgcatta
     9361 tcgcaggcga taacccggat gcggcgttgg cgttagaaaa agcccgcgcg ttcccaggtc
     9421 tgaatggcat ggatctggca aaagaagtga ccaccgcaga agcctatagc tggacacaag
     9481 ggagctggac gttgaccggc ggcctgccag aagcgaaaaa agaagacgag ctgccgttcc
     9541 acgtcgtggc ttatgatttt ggtgccaagc gcaacatcct gcggatgctg gtggatagag
     9601 gctgtcgcct gaccatcgtt ccggcgcaaa cttctgcgga agatgtgctg aaaatgaatc
     9661 cagacggcat cttcctctcc aacggtcctg gcgacccggc cccgtgcgat tacgccatta
     9721 ccgccatcca gaaattcctc gaaaccgata ttccggtatt cggcatctgc ctcggtcatc
     9781 agctgctggc gctggcgagc ggtgcgaaga ctgtcaaaat gaaatttggt caccacggcg
     9841 gcaaccatcc ggttaaagat gtggagaaaa acgtggtgat gatcaccgcc cagaaccacg
     9901 gttttgcggt ggatgaagca acattacctg caaacctgcg tgtcacgcat aaatccctgt
     9961 tcgacggtac gttacagggc attcatcgca ctgataaacc ggcgttcagc ttccagggtc
    10021 acccggaagc cagccctggg ccacacgacg cagcgccgtt gttcgaccac tttatcgagt
    10081 taattgagca gtaccgtaaa accgctaagt aatcaggagt aaaagagcca tgccaaaacg
    10141 tacagatata aaaagtatcc tgattctggg tgcgggcccg attgttatcg gtcaggcgtg
    10201 tgagtttgac tactctggcg cgcaagcatg taaagccctg cgcgaagagg gttaccgcgt
    10261 cattctggtg aactccaacc cggcgaccat catgaccgac ccggaaatgg ccgatgcgac
    10321 ctacatcgag ccgattcact gggaagtagt acgcaagatt attgaaaaag agcgcccgga
    10381 cgcggtgctg ccaacgatgg gcggtcagac ggcgctgaac tgcgctctgg agctggagcg
    10441 tcagggcgtg ttggaagagt tcggcgtgac tatgattggt gcgaccgccg atgcgattga
    10501 taaagcagaa gaccgccgtc gtttcgacgt agcgatgaag aaaattggtc tggaaaccgc
    10561 gcgttccggt atcgcacaca cgatggaaga agcgctggcg gttgccgctg acgtgggctt
    10621 cccgtgcatt attcgcccat cctttaccat gggcggtagc ggcggcggta tcgcttataa
    10681 ccgcgaagag tttgaagaaa tttgcgcccg cggtctggat ctctccccaa ccaaagagct
    10741 gctgattgat gagtcgctga tcggctggaa agagtacgag atggaagtgg tgcgtgataa
    10801 aaacgacaac tgcatcatcg tctgctctat cgaaaacttt gatgcgatgg gcatccatac
    10861 cggtgactcc atcactgtcg cgccagccca aacgctgacc gacaaagaat atcaaatcat
    10921 gcgtaacgcc tcgatggcgg tgctgcgtga aatcggtgtt gaaaccggtg gttccaatgt
    10981 ccagtttgcg gtgaacccga aaaacggtcg cctgattgtt atcgaaatga acccacgcgt
    11041 gtcccgttct tcggcgctgg cgtcgaaagc gaccggtttc ccgattgcta aagtggcggc
    11101 gaaactggcg gtgggttaca ccctcgacga actgatgaac gacatcactg gcggacgtac
    11161 tccggcctcc ttcgagccgt ccatcgacta tgtggttact aaaattcctc gcttcaactt
    11221 cgaaaaattc gctggtgcta acgaccgtct gaccactcag atgaaatcgg ttggcgaagt
    11281 gatggcgatt ggtcgcacgc agcaggaatc cctgcaaaaa gcgctgcgcg gcctggaagt
    11341 cggtgcgact ggattcgacc cgaaagtgag cctcgatgac ccggaagcgt taaccaaaat
    11401 ccgtcgcgaa ctgaaagatg ctggcgcaga gcgtatctgg tacatcgccg atgccttccg
    11461 tgcgggcctg tctgtggacg gcgtgttcaa cctgaccaat attgaccgct ggttcctggt
    11521 acagattgaa gaactggtgc gtctggaaga gaaagtagcg gaagtgggca tcactggcct
    11581 gaacgctgaa ttcctgcgcc agctgaaacg caaaggcttt gccgatgcgc gtctggcaaa
    11641 actggcgggc gtgcgcgaag cggaaatccg caagctgcgc gaccagtatg acctgcaccc
    11701 ggtttataag cgcgtggata cctgtgcggc agagttcgcc accgacaccg cttacatgta
    11761 ctccacttat gaagaagagt gcgaagcgaa tccgtctacc gaccgtgaaa aaatcatggt
    11821 tctcggcggc ggcccgaacc gtatcggtca gggtatcgaa ttcgactact gctgtgtaca
    11881 cgcctcgctg gcgctgcgcg aagacggtta cgaaaccatt atggttaact gtaacccgga
    11941 aaccgtctcc accgactacg acacttccga tcgcctctac ttcgagccgg taactctgga
    12001 agatgtgctg gaaatcgtgc gtattgagaa gccgaaaggc gttatcgttc agtacggcgg
    12061 tcagaccccg ctgaaactgg cgcgcgcgct ggaagctgct ggcgtaccgg ttatcggcac
    12121 cagcccggat gctatcgacc gtgcagaaga ccgtgaacgc ttccagcatg cggttgagcg
    12181 tctgaaactg aaacaaccgg cgaacgccac cgttaccgct attgaaatgg cggtagagaa
    12241 ggcgaaagag attggctacc cgctggtggt acgtccgtct tacgttctcg gcggtcgggc
    12301 gatggaaatc gtctatgacg aagctgacct gcgtcgctac ttccagacgg cggtcagcgt
    12361 gtctaacgat gcgccagtgt tgctggatca cttccttgat gacgcggtag aagttgacgt
    12421 ggatgccatc tgcgacggcg aaatggtgct gattggcggc atcatggagc acatcgagca
    12481 ggcgggcgtg cactccggtg actccgcatg ttctctgcca gcctacacct taagtcagga
    12541 aattcaggat gtgatgcgcc agcaggtgca gaaactggcc ttcgaattgc aggtgcgcgg
    12601 tctgatgaac gtgcagtttg cggtgaaaaa caacgaagtc tacctgattg aagttaaccc
    12661 gcgtgcggcg cgtaccgttc cgttcgtctc caaagccacc ggcgtaccgc tggcaaaagt
    12721 ggcggcgcgt gtgatggctg gcaaatcgct ggctgagcag ggcgtaacca aagaagttat
    12781 cccgccgtac tactcggtga aagaagtggt gctgccgttc aataaattcc cgggtgttga
    12841 cccgctgtta gggccagaaa tgcgctccac cggggaagtg atgggcgtgg gccgcacctt
    12901 cgctgaagcg tttgccaaag cgcagctggg cagcaactcc accatgaaga aacacggtcg
    12961 tgcgttgctt tccgtgcgcg aaggcgataa agaacgcgtg gtggacctgg cggcaaaact
    13021 gctgaaacag ggcttcgagc tggatgcgac ccacggcacg gcgattgtgc tgggcgaagc
    13081 gggcattaat ccgcgtctgg tgaacaaggt gcatgaaggc cgtccgcaca ttcaggaccg
    13141 catcaagaat ggcgaatata cctacatcat caacaccact tcaggccgtc gcgcgattga
    13201 agactccc
//
LOCUS       DAJTNN010000110        13092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTNN010000110 DAJTNN010000000
VERSION     DAJTNN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.110"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            287..568
                     /gene="ppiC"
                     /locus_tag="OO150_002791"
     CDS             287..568
                     /gene="ppiC"
                     /locus_tag="OO150_002791"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5516872.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(655..2130)
                     /gene="ilvC"
                     /locus_tag="OO150_002792"
     CDS             complement(655..2130)
                     /gene="ilvC"
                     /locus_tag="OO150_002792"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5516873.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2280..3170
                     /gene="ilvY"
                     /locus_tag="OO150_002793"
     CDS             2280..3170
                     /gene="ilvY"
                     /locus_tag="OO150_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5516874.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(3167..4711)
                     /gene="ilvA"
                     /locus_tag="OO150_002794"
     CDS             complement(3167..4711)
                     /gene="ilvA"
                     /locus_tag="OO150_002794"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5516875.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4714..6564)
                     /gene="ilvD"
                     /locus_tag="OO150_002795"
     CDS             complement(4714..6564)
                     /gene="ilvD"
                     /locus_tag="OO150_002795"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5516876.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6629..7558)
                     /gene="ilvE"
                     /locus_tag="OO150_002796"
     CDS             complement(6629..7558)
                     /gene="ilvE"
                     /locus_tag="OO150_002796"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5516877.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7578..7841)
                     /gene="ilvM"
                     /locus_tag="OO150_002797"
     CDS             complement(7578..7841)
                     /gene="ilvM"
                     /locus_tag="OO150_002797"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5516878.1"
                     /translation="MMQYQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(7838..9484)
                     /locus_tag="OO150_002798"
     CDS             complement(7838..9484)
                     /locus_tag="OO150_002798"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5516879.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9487..9537)
                     /locus_tag="OO150_002799"
     CDS             complement(9487..9537)
                     /locus_tag="OO150_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516880.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            complement(9624..9722)
                     /gene="ilvL"
                     /locus_tag="OO150_002800"
     CDS             complement(9624..9722)
                     /gene="ilvL"
                     /locus_tag="OO150_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5516881.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10074..11594
                     /locus_tag="OO150_002801"
                     /pseudo
     CDS             10074..11594
                     /locus_tag="OO150_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11619..11957)
                     /gene="maoP"
                     /locus_tag="OO150_002802"
     CDS             complement(11619..11957)
                     /gene="maoP"
                     /locus_tag="OO150_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5516882.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            12076..12915
                     /gene="hdfR"
                     /locus_tag="OO150_002803"
     CDS             12076..12915
                     /gene="hdfR"
                     /locus_tag="OO150_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5516883.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(13011..13086)
                     /locus_tag="OO150_002804"
     tRNA            complement(13011..13086)
                     /locus_tag="OO150_002804"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13051..13053),aa:Trp,seq:cca)
BASE COUNT         3020 a         3554 c         3399 g         3119 t
ORIGIN      
        1 tcttaatcaa tgtttgccac atgagttggt tgcagacata cagagggcgg tcatcaagtc
       61 aatcaatgcc atattgctca taaattgaca ataaaataac catccccgat aatgactttg
      121 tagcccatag aggccctaag ttttctcccc ttgagggacc tgcgaagccc ggattttccg
      181 ggcttttgcc atatatgtac cagcccctcc ctgactgaca attacgttag catctgcgga
      241 tcatgctaaa atcgccgcct gacaattatt cactcaaggc acgatcatgg caaaaacagc
      301 agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc tcgagcagat
      361 taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc catcaggcaa
      421 acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt tcgataaagt
      481 tgtgttctct tgtccggtac tggagccgac cggcccgctg cacacccagt tcggttacca
      541 catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccaggaga aggccttgag
      601 tgttttctcc ctctccctgt gggagagggt cggggtgagg gcatcagcgc gcacttaacc
      661 cgcaactgca atacgtttca tatccgtcat atagccgcgc agtttcttac ctacctgctc
      721 aatcgcatgg cagcgaatcg cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc
      781 ttccggaata gctttaccca gatcgcccgg ttgcagctct gccataaacg gtttcagcaa
      841 cggcacacaa gcgtaagaga acagatagtt accgtactca gcggtatcag agataaccac
      901 gttcatttcg tacagacgct tacgggcgat ggtgttggca atcagcggca gctcgtgcag
      961 tgattcataa tatgcagact cttcaatgat gccggaatcg accatggttt caaacgccag
     1021 ctcaacgccc gctttcacca tcgcgatcat cagtacgcct ttatcgaagt actcctgctc
     1081 gccgattttg ccttcatact gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg
     1141 ccaggtcagc agtttcttat catcgttggc ccagtccgcc atcataccgg aggagaattc
     1201 gccggagatg atgtcgtcca tatgtttctg gaacaggggt gccatgatct ctttcagctg
     1261 ttcagacagc gcgtaagcac gcagtttcgc cgggttagag agacggtcca tcatcagggt
     1321 gatgccgccc tgcttcagcg cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc
     1381 tgcgtatgcc ggatcggtgc cctcttccac cagcttgtcg aagcacagca gcgagccagc
     1441 ctgcaacata ccgcacagga tggtttgctc acccatcagg tcagatttca cttccgcaac
     1501 gaaggacgat tccagcacgc ccgcacggtg accaccggtt gctgccgccc atgctttagc
     1561 gatcgccatg ccttcgcctt tcggatcgtt ttccgggtga acggcaatca gcgtcggtac
     1621 gccgaatcca cgtttgtact cttcacgcac ttcggtgcca gggcatttcg gcgcaaccat
     1681 gactacggtg atgtctttac ggatctgctc gcccacttcg acgatgttga aaccgtgcga
     1741 gtaacccagc gccgcgccgt ctttcatcag tggctgtacg gtgcgcacta cgtcggagtg
     1801 ctgcttgtct ggcgtcaggt taaccaccaa atccgcctgc gggatcagtt cttcgtaagt
     1861 gcccacttta aaaccgtttt cggtcgcttt acgccaggat gcgcgcttct cggcaatcgc
     1921 ttctttacgc agagcgtagg agatatcgag accagaatca cgcatgttca ggccctggtt
     1981 cagcccctgt gcgccacagc cgacgataac cactttttta ccctgaaggt agctcgcgcc
     2041 gtcggcgaat tcgtcgcgcc ccataaagcg acatttgccc agctgtgcca gctgctggcg
     2101 caggttcagt gtattgaagt agttagccat ggtgattcct cgtgatgttg tgcttcttat
     2161 tgttcggttc gcgtttgcga gattgaattc actatatgac aggaaattta ttgcggaaat
     2221 tgatatattc acaacgtcac attgcaattt ctgcaacgtc aacattgagg gctgctccat
     2281 tggatttacg cgatctgaaa accttcctgc atctggcgga aagccgccat tttggccgca
     2341 gcgcgcgggc gatgcacgtt agcccatcca cgctctcacg gcagattcag cgcctggaag
     2401 aagatcttgg tcagccgcta tttgtgcgcg ataaccgcac ggtgacgctg actgaagcgg
     2461 gcgaagagct gcgcgttttc gcccagcaaa cgctgttgca gtatcagcag ttgcgccaca
     2521 ccatcgatca gcaagggccg tcgctctctg gcgaattaca tatcttctgc tcggtgaccg
     2581 ctgcctacag ccatctgcca ccgattctcg accgcttccg cgcggaacac ccgtcggtgg
     2641 agatcaaact aaccactggt gatgcggcag atgcgatgga aaaggtagtc actggcgaag
     2701 cggatctggc gattgccggg aaaccggaga ctctgcccgg cgcagtggcg ttttcgatgc
     2761 tggagaatct ggcagtagtg ctgattgcgc ccgcgctgcc ctgcccggtg cgtaaccagg
     2821 tttcgtcaga gaagccggac tggtctacag tgccgtttat catggccgat cagggaccgg
     2881 tacgccgccg cattgaactg tggtttcgac gcaataaaat cagcaacccg atgatttacg
     2941 ccacggttgg cgggcatgaa gcgatggtat cgatggtggc gctcggctgt ggcgtggcgc
     3001 tgttgccgga agtagtgctg gaaaacagcc ccgaaccggt gcgtaaccgc gtgatgattt
     3061 tagagcgcag cgatgaaaaa acgccgtttg aactgggcgt ttgtgcgcag aaaaagcggc
     3121 tgcatgagcc gctgattgag gcgttctgga agattttgcc gaaccattaa cccgccaaaa
     3181 agaacctgaa cgccggatta ttggtttcgt cgtggcaatc gtagcccagc tcatttagcc
     3241 gggtttcgaa atccggttca tggtcgccga gttcgaacgc cgccagtacg cgcccgtagt
     3301 cggtgccatg gctgcgatag tggaacaaag aaatgttcca gtgcgtaccc agcgtgttga
     3361 ggaagcgcag cagcgcgccc ggtgattccg ggaattcgaa gctgtagagg cgttcctgca
     3421 acggatgtga cggacgcccg ccgaccatat agcgcacatg caacttcgcc atttcgtcgt
     3481 cggagagatc caccacgctg tagccgccgt cgttgagcat ctgcaaaatt tctttgcgct
     3541 cttcgaggcc gcggctcagg cgcacgccga caaagatgca ggcgtttttg gcatcggcaa
     3601 aacggtagtt gaactcggtg accgctcgcc cgccaagcag ttggcagaat ttgaggaagc
     3661 tgcctttttc ttccggaatg gtcaccgcca acaacgcttc acgctgttcg cccagttcgc
     3721 agcgttccga gacgtagcgc aggccgtgga agttcacgtt ggcaccggaa agaatatgcg
     3781 ccagccgttc gccgcgaatg ttgtgctggg cgatatattt tttcattccc gccagcgcca
     3841 gcgcgccaga gggttccgcc accgcgcgca catcttcgaa taaatccttc atcgccgcac
     3901 agatcgcatc actatcgacg gtgatgatgt cgtcgagata ctcctggcat aaacggaagg
     3961 tttcgtcacc gatgcgtttt accgctacgc cttcagcaaa tagccctacg cgcggcagat
     4021 caaccggatg acccgcatcc agcgctgctt tcaggcaggc ggagtcttcc gcttctacgg
     4081 cgatcacttt gatttgtggc atcagttgtt tgatcagcac cgcaacgccc gccgccagac
     4141 cgccgccgcc gactggcaca aatacgcggt cgagatgggc gtcctgctgg agcagttcca
     4201 gcgccagcgt gccttgcccg gcaatcacca tcggatggtc gaacggcggc acccaggtga
     4261 acccctgctg ctgtgacagt tcgatcgctt tagctttcgc ttcgtcaaag ttcgcgccat
     4321 gaagcagcac ttcgccgccg aatccacgta ccgcatcgac tttgatgtcg gcggtggcgg
     4381 ttggcataac gatcagggcc ttcacgccta accgcgcaga agaaaacgcg acgccctgcg
     4441 cgtggttacc cgcagaagca gtgatcacgc cgtgcgcttt ttgttcttcc gtcaggcccg
     4501 ccatcatggc gtatgcgccg cgcagcttaa agctgtgcac tggctggcga tcttcgcgct
     4561 tcaccagaat gacgttatca agacgcgacg acagtttttc cattttttgt agcggcgtaa
     4621 cctgcgctgc ctcgtaaacc ggcgcgcgca gcactgctct taaatattcg gcaccttccg
     4681 gagcaccaga caggggttgc gagtcagcca ttattaaccc cccagtttcg atttatcgcg
     4741 caccgcacct ttgtcggcgc tggttgccag gctggcgtag gcgcgcaggg caaaggaaac
     4801 ctgacgttca cggtttttcg gcgtccaggc tttgtcaccc cgagcttcct gcgcttcacg
     4861 acgcgccgcc agttcggcat cgcttacctg taactgaatg ccacggttcg ggatgtcgat
     4921 agcgatcagg tcaccatctt caatcagacc aatgctgccg ccgcttgccg cttccggtga
     4981 gacgtggccg atggaaagac cagaggtgcc gccagagaaa cgaccgtcgg tgatcagcgc
     5041 acaggcttta ccgagaccca ttgatttcag gaagctggtt gggtagagca tttcctgcat
     5101 gccagggccg cctttcgggc cttcatagcg gatcaccacc acatcgcccg cgacaacttt
     5161 gccaccgagg atcgcttcta ccgcgtcgtc ctggctttcg tacacttttg ccgggccggt
     5221 gaatttgagg atgctgtcat cgacgcccgc ggttttcacg atgcagccgt tttcagcaaa
     5281 attaccgtag agcaccgcca ggccgccgtc tttgctgtag gcgtgttcca gcgagcggat
     5341 acagccgttt gcgcgatcgt cgtccagcgt atcccaacgg caatcttgcg agaatgcctg
     5401 ggtggtacgg atacccgcag ggccggcgcg gaacatattt tttaccgcgt catcctgggt
     5461 cagcataacg tcgtattgct ccagcgtttg cggcaacgtc aggccgagca cgtttttcac
     5521 atcacggttc agtaaccccg cgcgatccag ttcgccgaga ataccgataa caccaccagc
     5581 acggtgaaca tcttccatat ggtatttctg ggtgctcggc gcaactttac acagctgtgg
     5641 aaccttgcgg gaaagcttat cgatatcact catggtgaag tcgatttccg cttcctgcgc
     5701 cgccgccagc aggtgaagta cggtgttagt cgatccaccc atcgcgatat ccagcgtcat
     5761 ggcgttttca aacgccgcct tactggcgat attacgcggc agtgcacttt cgtcgttttg
     5821 ctcgtaataa cgtttggtca attcaacaat gcgtttacca gcattaagga acagctgctt
     5881 acggtccgcg tgggttgcca gcagcgagcc gttgcctggc tgcgacaaac ccagcgcttc
     5941 ggtcaggcag ttcattgagt tagcggtaaa catcccggag caggaaccgc aggttgggca
     6001 ggcggaacgt tcaacctgat cgctctggga gtcagaaact ttcgggtctg cgccctggat
     6061 catcgcatca accagatcga gtttgattat ctgatcggac agtttggttt tcccggcctc
     6121 catcgggccg ccggaaacaa agatcaccgg aatattcagg cgcagggaag ccatcagcat
     6181 ccccggggtg attttgtcgc agttagagat gcagaccatg gcgtcggcgc agtgggcgtt
     6241 gaccatatac tcaacggaat cagcgatcag ttcgcgagat ggaagtgaat aaagcatccc
     6301 cccgtggccc atggcaatcc catcatccac cgcaatggtg ttgaactctt tggcaacgcc
     6361 gccagccgct tcaatttgtt cggcgaccag tttaccgaga tcgcgcagat ggacgtgacc
     6421 cggtacaaat tgggtgaacg agttcacaac cgcgataatc ggcttgccaa aatcggcgtc
     6481 ggtcattccg gtggcgcgcc acagcgcacg agcacccgcc atattacgac catgagtggt
     6541 ggtggcggaa cggtacttag gcatacttta tttactccca gtgtctgtct cgtaaatggg
     6601 acggtgcgtg ccgtcccatt ttttgtattt attgattaac ttgatctaac cagccccatt
     6661 tatcttcggt ttcgccagtg aagaggccga agaaggcttg ctgaatgcgt ttggtaaccg
     6721 ggccacaacg gccttcgcca acctgaatac cgtctacgct gcgcactggc gtgatttctg
     6781 ccgcagtacc ggacataaac acttcatccg ccaggtacag ggattcgcgc gacagcacct
     6841 gctcacgcac ttcaattccc agctctttcg ccagtttgat gatggcatca cgggtaatac
     6901 ccggcagcgc ggatgaggta aacggtgggg tgaacagcac gccgtctttc acttcaaaca
     6961 ggttttcacc tgcgccttca gagatgtaac cgttcacatc cagcgcgata ccttcctgat
     7021 aaccgtggcg gcgcgcttcg ctacccacca gcagggaaga gaggtagtta ccaccggctt
     7081 ttgccgccgt cgggatggtg tttggtgctg cgcggttcca ggaggaaacc atcgcatcga
     7141 tcccctgctc cagcgcttct gcgcccagat acgctcccca cgggaaagca gcgataatca
     7201 cgtcggttga gtatcccgct ggcgggttta ctcccatgcc aacatcaccg acgaagatca
     7261 gcggacggat ataggcgctg gtgagattat ttttgcggat cacgtcacga caagcttcca
     7321 tcagctcatc aatgctctgc gaaaccggga agcgatagat tttggcggag tcatgcagac
     7381 gctgcatatg ctcacgatgg cggaatacaa ccggtccttt gtgcgagtcg tagcaacgga
     7441 tgccttcaaa aaccgaagtg ccatagtgca gcgcgtgcga catcacatgc accttcgcgt
     7501 cttcccagcg aaccatctcc ccattgaacc aaatgtaatc agctttcttc gtggtcattt
     7561 ttatattcct tttgcgctca ggcgcggatt tgttgtgatg tggttgtgct ctggcagatg
     7621 gcaacgtgtg cgacgtccac cagtttattt aactgactaa acagtaagtc gaccgaccgt
     7681 gggctggcaa cggtcaattc gatatttata ttttgtgcat cgctggcggc ggccatattc
     7741 attgagcaga cgtggaaacc acgatgacgc accacgcgta aaacacgttc taaggtttcc
     7801 ggattgaagc gagccgatac attgacctga tattgcatca tgataatttc tccaacattt
     7861 ctgaattact ggcgccaggc ggcaccagcg gccagacgtt ctcaagttcg tcgattgaga
     7921 catgaagcag gtatggccca tcactgttca gcatggtgtc gagtgccgct tcaacctggt
     7981 ctttacgggt gatgtgttgg ccagggatgc cgaaggcgct ggctaacatg aggaaatcgg
     8041 ggttatcagt aagggtggtt tcgctgtatc gttcctgaaa aaacagttgc tgccattgtc
     8101 gaaccatccc taaccgttgg ttatcgagta agacgatttt caacggtaac tgcttgcgtt
     8161 ttacggtgcc cagctcttgc acattcatca tgaaagagcc gtcaccggag atacagacga
     8221 cggtatcgtt cggtcgcgcg acttgtgcgc caaccgccgc tggtaaacca aaacccatag
     8281 tgcctaagcc gctggaggtg atgaaatttt ccgggcgagt gtgggcgatg tgttgcgcag
     8341 cccacatctg gtgctgcccc acatctgtgg tcacgacgca atccgcaggt ttacgatccg
     8401 acagttgttt taacaacaac ggcgcgtaga tagcgtcgcc gggatggtcg taacgccagg
     8461 catgttcatc acgcagctgc gcgcagtatt gctgccagtc attgatattt aacggctgct
     8521 gtaattctgg taacagagca tttaaatcac cttgtaatgc cacatgtgcc tgacgcagct
     8581 tgttcatttc tgccgggtcg atatccatat ggataacgct ggcgtgtggt gcgaaggtgt
     8641 tcagtttgcc ggtcacccgg tcatcaaaac gtgcacccac ggcgatcagc aagtcgcact
     8701 cctgcaccgc gaagttcgcc gctttggtgc catgcattcc cagcatgccc agatagtacg
     8761 gataatctgc ttcaactgcg cccagccctt tcagcgtgca ggtggcaggc atttttgtgg
     8821 tagcgagaaa ttctcgtaaa gcaggaactg cctgcgccat acccacgcca ccaccaacgt
     8881 acagcatcgg tttttgcgct tttgccagca tctggcgcgc ttgctcaact tcggcatgtg
     8941 ggaaagtcac ttcgttttca acggtggtga accacggttc caggtcaccg ctggctagct
     9001 ggatatcttt tgggatatcg accagaaccg gaccaggacg acctgagctg gcaacgtcga
     9061 atgcttcagc cataatgcgc ggcaactctt ccagcgactg caccagaaag ctgtgcttgg
     9121 tacaggctaa cgacaatccc aggacatcca cttcctgaaa tgcatcagtg ccgataaacg
     9181 gtgcggacac ttgaccggtg atggcaacaa cagggataga atctaacagt gcgtccgcaa
     9241 gcccggttat caggttggtt gcgcccggac cagacgtggc gatacatacg ccagttttgc
     9301 cggtagcacg ggcataaccg atagccgcca ttgccgcacc ctgctcatgt cggcacagca
     9361 agtgctccac gccgccgtca tacaatgcat cgtaaaccgg cataattgcg ccacccggat
     9421 aaccgaaaac ggtgttcaca ccctgtgccc gcaacgcatg taccacccac tgtgcgccat
     9481 tcatagttag ttccccgtgc tgaatgttga gaaacagaat tttgtgctgt tattcattgt
     9541 ctgctcctcg gttatgtttt taaggtcaaa aaaaaccccc ggacctttcg gtgcgggggt
     9601 cttagttcgt taaggcttga tttctaagcc tttcctcgtc caagtgcagc cccgcacggt
     9661 gggataataa tcaccaccac gctaatcacg accaggctaa tcactcgtag aagggctgtc
     9721 attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca taggttttgt
     9781 aaatagtaca agatattttt tggccgattt ttatgatgcg tttaataatt tcaatataag
     9841 ttgttgtttt ttaaaaataa aatagtgagt aaaaaaattc aatttcagta tgaatgttgt
     9901 tcccttcctc gtagttcatc ctttgtccgc tgcatttttg cgagcatcat tccaccgcca
     9961 gaacaactca acttcacttg cacaaataaa caggaatcag ccactaaaat tcacgaatta
    10021 gcgggctaca gcagcgatat cttatgcaca taatggtgac tcaaggaggg cttatgtcac
    10081 tgtcaattgt tcatacccgc gcagccctgg gagtaaatgc gcccccgatc actgttgagg
    10141 tacatatcag taaaggtcta cccggcttaa cgaaggtggg cttaccagaa acaacggtaa
    10201 aagaagctcg cgatcgcgtg cgcagcgcca ttatcaatag cggatatgaa tatccggcga
    10261 aaaaaatcac catcaacctg gctccagctg atctgccaaa agaaggggga cgatatgatt
    10321 tacctatcgc cattgcgttg ctggcggcct cagaacagct tacagccaat aagttagatg
    10381 aatatggatt agtcggagaa ctggcgctta caggcgctct gcgtggcgtt cccggcgcaa
    10441 tctccagtac aactgaagct attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg
    10501 aagatgaagt ggggctaatt aacggtgaag gatgcctgat agccgatcat ctgcaagctg
    10561 tctgtgcgtt tctggaaggt aagcacgctc tcgaacgccc gaaaccaact gatacagtat
    10621 cccgggcgct acaacatgat ctcagtgatg ttatcggtca ggaacaagga aagcgaggac
    10681 tggaaattac cgccgctggc gggcacaacc ttttactgat tgggccgccg ggaacaggta
    10741 aaacaatgct cgccagccgt attaatggcc tgttgccaga tttaagcaat gaagaggcac
    10801 tggagagtgc tgcgatatta agtctggtaa atgctgaatc agtacaaaaa caatggcggc
    10861 agcgcccgtt ccgctcacct catcacagtg catcgttaac tgcgatggta ggcggtggcg
    10921 caattccagg gcccggtgaa atttcgctgg cgcataacgg cgtgcttttt cttgatgagt
    10981 tacctgaatt tgaaaggcgt acactggatg ccttgcgaga gccgattgaa tccgggcaga
    11041 tccatctttc acgcacgcga gcaaaaataa cctatccagc ccgtttccag cttgtcgcgg
    11101 cgatgaatcc cagccctacc ggacattatc agggaaacca taaccgctgc acaccagagc
    11161 aaacgctgcg ttatctcaac cgactctctg gtccctttct cgaccgcttc gatctctcac
    11221 tggagatccc attaccgcct cccggtattt tgagtaaaac ggtagtgccg ggagaaagca
    11281 gcgccaccgt taaacaacgc gtaatggccg ccagagagcg ccaatttaag cggcagaata
    11341 aactgaacgc ctggctggat agtccggaaa tacgccaatt ctgcaaactt gagagcgaag
    11401 atgcgatgtg gctggaagaa acgctgatcc atctggggtt atcgattcgt gcctagcagc
    11461 ggttattgaa agttgcacga accattgctg atattgatca gtctgacatt atcacacgtc
    11521 aacatttgca ggaggcagtt agctatcgag cgattgaccg tttgctcatc catctgcaga
    11581 aactactgac ataaaaaaag ggcatttcgc cctttttatt aatcgtcaga atcggtgtag
    11641 tcttcagcac cttcaacctg cggtttaccg ccggaaaggg tgtgaaaacg ttttggacgc
    11701 ttaatacgcg tcatatactt ggaccacacg cgttctgctt ctgtcactgg ctcacgttcg
    11761 ccacggcata ctgctacgaa gagtttctct tcctcggtaa ccggctcgcg tttgccaaga
    11821 tccaactcat tgaaggcata accatgacgc tcaagcagtt gtgcctcttt gatggtgaaa
    11881 tcaccatgac gagagaatcc acgtggataa tgtttattgt cgaaatatcg attagtcgtc
    11941 gtaaagcttt ccgccatcct gcacgctcct aattctttga ccgagctagt tatggcgcgg
    12001 agtattagtt acgcttgaca gagtgtaaaa caaaacattt aaatcataac gacaaataat
    12061 tttgcggaga gcactgtgga tacggaattg ttaaaaactt tcctggaagt tagccgaacg
    12121 cgtcactttg gtcgagcggc tgaatcgctc tatctgaccc agtcagcagt gagctttcga
    12181 atcagacaac tggaaaatca actgggtgtg aaccttttca cccgccacag aaacaatatc
    12241 cgtttaaccg ctgccggtga aaaactactg ccttatgcag aaacgctcat gagcacctgg
    12301 caggccgccc gcaaggaggt ggcgcatacc tcacgacata acgagttttc tatcggtgcc
    12361 agcgcctcat tgtgggaatg tatgcttaat cagtggctgg gacgtttgta tcaaaatcag
    12421 gatgcccata caggcttaca gttcgaagcg cgaattgccc aacggcagtc tctggtaaaa
    12481 cagctgcatg aacgccagct tgatcttctt attaccactg aagcgcccaa aatggacgaa
    12541 tttagtagtc agttgctggg atatttcact ttagcgcttt ataccagtgc cccttcaaaa
    12601 ctaaagggag atcttaatta tctgcgactt gagtgggggc cagattttca acagcatgag
    12661 gcaggtttga tcggtgctga cgaagtgccc attctgacaa ccagttctgc tgaactggca
    12721 cagcaacaga ttgcgatgct taatggttgc acctggctac ccgtcagctg ggcgcgtaaa
    12781 aaaggcggcc tgcataccgt tgtcgatagc acaacacttt cacggccgct ttatgccata
    12841 tggctgcaaa atagcgataa aaatgcgttg attcgcgatc ttttgaaaat taacgtgctg
    12901 gatgaagtgt attaatatga atggctggca aggatgccgg tagaaggatt tacttcggag
    12961 agggttattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggcaggggc
    13021 ggagagactc gaactcccaa cacccggttt tggagaccgg tgctctacca attgaactac
    13081 gcccctaatt ag
//
LOCUS       DAJTNN010000111        13087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTNN010000111 DAJTNN010000000
VERSION     DAJTNN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.111"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            176..1150
                     /gene="cysB"
                     /locus_tag="OO150_002805"
     CDS             176..1150
                     /gene="cysB"
                     /locus_tag="OO150_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5516884.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QTRYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1445..1609
                     /locus_tag="OO150_002806"
     CDS             1445..1609
                     /locus_tag="OO150_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5516885.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1612..1779
                     /locus_tag="OO150_002807"
     CDS             1612..1779
                     /locus_tag="OO150_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516886.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1893..1988
                     /locus_tag="OO150_002808"
     CDS             1893..1988
                     /locus_tag="OO150_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516887.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2152..4827
                     /gene="acnA"
                     /locus_tag="OO150_002809"
                     /pseudo
     CDS             2152..4827
                     /gene="acnA"
                     /locus_tag="OO150_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="OO150_002810"
     CDS             complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="OO150_002810"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5516888.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5651..6415
                     /gene="pgpB"
                     /locus_tag="OO150_002811"
     CDS             5651..6415
                     /gene="pgpB"
                     /locus_tag="OO150_002811"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5516889.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLLWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6564..6872
                     /gene="lapA"
                     /locus_tag="OO150_002812"
     CDS             6564..6872
                     /gene="lapA"
                     /locus_tag="OO150_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5516890.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6879..8048
                     /gene="lapB"
                     /locus_tag="OO150_002813"
     CDS             6879..8048
                     /gene="lapB"
                     /locus_tag="OO150_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5516891.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNRARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8241..8978
                     /gene="pyrF"
                     /locus_tag="OO150_002814"
     CDS             8241..8978
                     /gene="pyrF"
                     /locus_tag="OO150_002814"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5516892.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8978..9304
                     /gene="yciH"
                     /locus_tag="OO150_002815"
     CDS             8978..9304
                     /gene="yciH"
                     /locus_tag="OO150_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5516893.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="OO150_002816"
     CDS             complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="OO150_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5516894.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9917..10666)
                     /locus_tag="OO150_002817"
     CDS             complement(9917..10666)
                     /locus_tag="OO150_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5516895.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(10756..10929)
                     /locus_tag="OO150_002818"
     CDS             complement(10756..10929)
                     /locus_tag="OO150_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516896.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="OO150_002819"
     CDS             complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="OO150_002819"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5516897.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKSEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         3329 a         3081 c         3291 g         3386 t
ORIGIN      
        1 tcgcgaaagc ggcccttttt tattgcatat tattttttct tcacacctat acactaaggc
       61 tataaatgat atagtggtta tagttagcac cttttttatt attaaatcgt attagtcacc
      121 cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta agtggatggt ttaacatgaa
      181 attacaacaa cttcgctata ttgttgaggt ggtcaatcat aacctgaatg tctcatcaac
      241 agcggaagga ctttacacat cacaacccgg gatcagtaaa caagtcagaa tgctggaaga
      301 cgagctaggc attcaaattt tttcccgaag cggcaagcac ctgacgcagg taacgccagc
      361 agggcaagaa ataattcgta tcgctcgcga agtcctgtcg aaagtcgatg ccataaaatc
      421 ggttgccgga gagcacacct ggccggataa aggctcactg tatatcgcca ccacgcatac
      481 ccagacacgc tacgcattac caaacgtcat caaaggcttt attgagcgtt atcctcgcgt
      541 ttctttgcat atgcaccagg gctcgccgac acaaattgct gatgccgtct ctaaaggcaa
      601 tgccgatttc gctatcgcca cagaagcgct gcatctgtat gaagatttag tgatgttacc
      661 gtgctaccac tggaatcggg ctattgtagt cactccggat cacccgctgg caggcaaaaa
      721 agccattacc attgaagaac tggcgcaata tccgttggtg acatatacct tcggctttac
      781 cggacgttca gaactggata ctgcctttaa ccgcgcaggg ttaacgccgc gtatcgtttt
      841 cacggcaacg gatgctgacg tcattaaaac ttacgtccgg ttagggctgg gggtaggggt
      901 cattgccagc atggcggtgg atccggtcgc cgatccggac cttgtgcgcg ttgatgctca
      961 cgatatcttc agccacagta caaccaaaat tggttttcgc cgtagtactt ttttgcgcag
     1021 ttatatgtat gatttcattc agcgttttgc accgcattta acgcgtgatg tcgttgatgc
     1081 ggctgtcgca ttgcgctcta atgaagaaat tgaggtcatg tttaaagata taaaactgcc
     1141 ggaaaaataa tttcttgcgt tattttcggc accttttatg tagcgaaggt gccggaatat
     1201 attctctttt gttacttatt tttataccgt gcacaatatt tttatccctc atatttatag
     1261 ggtaaattac ctgtaaagta gtgtctaatt acccgcctcg caaattccag ttttcaaata
     1321 tctatttcag gtcaaaagat tgaattattc actggagacg attcgtaaat tcgctggatt
     1381 tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc aatcgttcaa
     1441 cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg gaaatcagga
     1501 accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc ttggttctgc
     1561 acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac tatggtcggt
     1621 caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac agaaacgaaa
     1681 cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca gcaagccttt
     1741 atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt cttattaccc
     1801 atttttaatg aattaaaggg cttttaatac accgcagcaa taacagcttg agttatctca
     1861 acacaaaata ataaccgtta agggtgtagc ctatgatcaa cacaaatatg aaatattggt
     1921 cctggatggg cgcgttttct ctgtcgatgc tcttctgggc cgaactcctc tggatcatta
     1981 ctcactgatc cttgaccccg ctgcggcggg gttgtcattt gctttgccac aaggtttctc
     2041 ctcttttatc aatttgggtt gttatcaaat cgttacgcga tgtttgtgtt atctttaata
     2101 ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc tatgtcgtca
     2161 accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta ccactactac
     2221 agcctgccgc ttgctgctaa atcactgggc gatatcaccc gtctacccaa gtcactcaaa
     2281 gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga agaggatatc
     2341 cacgcgctgg caggatggct gaaaaatgcc catgctgacc gtgaaattgc ctaccgcccg
     2401 gcaagggtgc tgatgtagga ctttaccggc gtacctgccg ttgttgatct ggcggcaatg
     2461 cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct ctcaccggtc
     2521 gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga ggcatttgaa
     2581 gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt atgtgttcct gaaatgggga
     2641 aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca caggcatttg ccatcaggtt
     2701 aacctcgaat atctcggcaa agcagtgtgg agtgaattgc aggacggtga atggattgct
     2761 tatccggata cactcgttgg tactgactcg cacaccacta tgatcaacgg ccttggcgtg
     2821 ctggggtggg gcgtgggtgg gatcgaagca gaagccgcaa tgttaggcca gccggtttcc
     2881 atgcttatcc cggacgtagt gggctttaaa cttaccggta aattacgtga aggtattacc
     2941 gccacagacc tggttctcac tgttacccaa atgctgcgca aacatggcgt ggtggggaaa
     3001 ttcgtcgaat tttatggtga tggtctggat tcactaccgc tggcggatcg cgccaccatt
     3061 gccaatatgt cgccagaata tggtgccacc tgtggcttct tcccaatcga tgctgtaacc
     3121 ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg ttgagttggt cgaaaaatat
     3181 gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac cgatttttac cagtacgtta
     3241 gaactggata tgaatgacgt tgaagcgagc ctggcagggc caaaacgtcc acaggatcgc
     3301 gttgcactgc ccgatgtacc aaaagcattt gccgccagta acgaactgga agtgaatgcc
     3361 acgcataaag atcgccagcc ggtcgattat gttatgaacg gacatcagta tcagttacct
     3421 gatggcgctg tggtcattgc tgcgataacc tcgtgcacca acacctctaa cccaagtgtg
     3481 ctgatggccg caggcttgct ggcgaaaaaa gccgtaactc tgggcctcaa gcggcaaccg
     3541 tgggtcaaag cgtcgctggc accgggttcg aaagtcgttt ctgattatct ggcaaaagcg
     3601 aaactgacac cgtatctcga cgaactgggg tttaaccttg tgggatacgg ttgtaccacc
     3661 tgtattggta actctgggcc gctgcccgat cctatcgaaa cggcaatcaa aaaaggcgat
     3721 ttaaccgtcg gtgcggtgct gtccggcaac cgtaactttg aaggccgtat ccatccgctg
     3781 gttaaaacta actggctggc ctcgccgccg ctggtggttg cctatgcgct ggcgggaaat
     3841 atgaatatca acctggctgc tgagcctatt ggtcatgatc gtaaaggcga cccggtttat
     3901 ctgaaagata tctggccatc ggcacaggaa attgcccgtg cggtagaaca agtctccaca
     3961 gaaatgttcc gtaaagagta cgcagaagtt tttgaaggca cagcagagtg gaaggaaatt
     4021 aacgtcacac gatccgatac ctacggttgg caggaggatt caacctatat tcgcttatcg
     4081 cctttctttg atgaaatgca ggcaacacca gcacctgtgg aagatattca cggtgcgcgg
     4141 atcctcgcaa tgctggggga ttcagtcacc actgaccata tctctccggc gggcagtatt
     4201 aagcccgaca gcccggcggg tcgatatcta caaggtcggg gtgttgagcg aaaagacttt
     4261 aactcctacg gttcgcggcg tggtaaccat gaagtgatga tgcgcggcac cttcgccaat
     4321 attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg ggatgacgcg gcatttacct
     4381 gacagcgatg ttgtctctat ctatgatgcc gcgatgcgct ataagcagga gcaaacgccg
     4441 ctggcggtga ttgccgggaa agagtatgga tcaggctcca gccgtgactg gacggcaaaa
     4501 ggtccgcgtc tgcttggtat tcgtgtggtg attgccgaat cgtttgaacg aattcaccgt
     4561 tcgaatttaa ttggcatggg catcttgccg ctggaatttc cgcaaggcgt gacgcgtaaa
     4621 acgttagggc taaccgggga agagaagatt gatattggcg acctgcaaaa tctacaaccc
     4681 ggcgcgacgg ttccggtgac gcttacgcgc gcggatggta gccaggaagt cgtaccctgc
     4741 cgttgtcgta tcgacaccgc gacggagttg acctactacc agaacgacgg cattttgcat
     4801 tatgtcattc gtaatatgct gaagtaacaa gtatttgctt gccggttatt ttgcttccgg
     4861 caagcaaatt aattccacaa tgcgagaggg ttatttgttc agcaaatgac ccattttctc
     4921 ggctttggta tcgagataat gttcgttatt ggggttacga cctacaatca atggtacgcg
     4981 ttcaacaata ttaatccctg cttcggtcag aatttcgact tttttcgggt tattggttaa
     5041 caagcggact tcattgacgc caaggagttt gaacatatca gcgcaaagag tgaagtcgcg
     5101 ctcatcagcg gcgaagccta actggtggtt agcctctacg gtatcgtaac cttgatcctg
     5161 cagtgcgtaa gcgcggattt tattcagcag accaatgtta cgaccttcct gacggtgata
     5221 cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat gccgcttcga gctggaagcc
     5281 acaatcgcag cgcaagctga acagggcgtc accggtcaga cattcggaat ggacgcgcgc
     5341 aagtaccggg gtatgcccgg aaatatcgcc atagactagc gcgacatgat cgtgtccggt
     5401 tgccagttct tcaaatccca ccatcaggaa atcgccccat ggggttggca gtttggcttc
     5461 tgccacacgt ttaagctgca tgaaattctc cagataatgc tggttctgta ttggcttatt
     5521 ttgccataac gagaaggggt tcacctaatc acgacgcgtc gatcgttcac ggaatggcac
     5581 aaatctgtca atttttcctg gaactggcgt tttcagttat gattgtggga cttatcaaaa
     5641 aggagaggcc atgcgttcga ttgccagacg taccgcagtg ggagctgcac tattgcttgt
     5701 catgccagta gccgtatgga tttctggctg gcgttggcaa cctggagaac aaagttggct
     5761 actaaaagcg gctttttggg ttactgaaac tgtcacccag ccctggggcg tcattacaca
     5821 tttgatttta ttcggctggt ttctctggtg tctgcgtttt cgcattaagg ctgcctttgt
     5881 attatttgcc attctggcgg ccgcaatcct tgtgggacaa ggcgttaaat cctggatcaa
     5941 agacaaagtc caggaaccac gaccttttgt tatctggctg gaaaaaacac atcatattcc
     6001 ggttgatgag ttctacactt taaagcgagc agaacgcgga aatctagtga aagaacagtt
     6061 ggctgaagag aaaaatatcc cacaatattt gcgttcacac tggcagaaag agacggggtt
     6121 tgcctttcct tccggtcaca cgatgtttgc tgccagttgg gcactgctgg ccgttggttt
     6181 gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg ctgctctggg caacgggagt
     6241 catgggaagc cgcctgctgc tcgggatgca ttggccacgc gatctggtag tagctacatt
     6301 gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg caacgaattt gtgggccatt
     6361 aacaccacct gcggaagaaa atcgcgaaat agcgcaacga gaacaagaaa gttaacgctg
     6421 gttgattttc cgaatttagc ccttaaatca tcaacaatgc gcgtggatgc catttcgcag
     6481 acggcgcgaa aatggtactt taaagggcta ttgcggtaag ttgaccataa tttattcgct
     6541 ctaaccacat aacgggaagt aatgtgaaat atttactcat tttcttactg gtgttagcga
     6601 tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca acaggtgacg tttaattatc
     6661 tgttagcgca aggggagtac cgtatttcca cattgctggc ggtattgttt gctgcggggt
     6721 ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg agttcgtgtt tccctggcgc
     6781 gcgctgaacg taaaataaag cgactggaaa accagctttc acccgcgact gacgtggctg
     6841 tagtgccgca ctcgtcagcg gcgaaggaat aactttctat gctggagttg ttgtttctgc
     6901 ttttgcctgt agccgctgcc tatggctggt atatgggccg cagaagtgcg caacaaaaca
     6961 agcaagatga agccaaccgc ttgtcgcgtg attacgtagc gggggttaac ttcctgctta
     7021 gtaatcaaca ggataaagcg gtagacctgt ttctcgatat gcttaaagag gatacaggca
     7081 ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc gcgtggcgaa gttgatcgcg
     7141 ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct gacctatgaa cagcgtctgt
     7201 tggcgattca acaactaggg cgtgattaca tggccgccgg gttatacgac cgcgcggaag
     7261 acatgttcaa tcagctgacc gatgaaactg acttccgcat tggcgctctg caacagttgc
     7321 tacaaatcta ccaggctacc agtgagtggc agaaagcaat tgatgttgcc gaacgcctgg
     7381 tgaagctggg taaagataaa cagcgcgtcg aaattgccca tttctactgt gagttagccc
     7441 tgcagcatat ggccagcgac gatctcgatc gtgccatgac cttgctaaaa aaaggggcgg
     7501 cggcagataa aaacagagcc cgcgtatcca tcatgatggg acgcgtgttt atggcgaaag
     7561 gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat atcccaggac agagaactgg
     7621 tcagcgaaac gctggaaatg ttgcaaacct gctatcagca gttgggtaaa actgccgaat
     7681 gggcagaatt cctgcaacgc gcggtggaag agaacaccgg tgccgatgct gaattgatgc
     7741 ttgctgatat catcgaagcg cgcgacggta gtgaggccgc acaggtctat attacgcgcc
     7801 agcttcagcg tcatccgacc atgcgtgtgt tccataagtt aatggattac cacttaaatg
     7861 aagcggaaga agggcgtgcc aaagagagcc tgatggtgct gcgtgacatg gttggcgaaa
     7921 aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg ttttaccgca tacaccctct
     7981 actggcattg tccgtcttgt cgggcctggt caaccattaa accgattcgc ggtcttgatg
     8041 gcctgtaatt tttaaaaaaa tccgacttta gttacaacat gctagttatt gatgttccat
     8101 tgtgctccgg caacgacgga gcagaaaaag cctgccaggg gaaaaatcgc aactgttaat
     8161 tttttatttc cacgggtaga atgctcgccg tttacctgtt tcgcgccact tccggtgccc
     8221 atcatcaaga aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc gctgttacga
     8281 attctcctgt ggttgttgcc cttgattatc ataatcgtga tgacgcgctg tcctttgtcg
     8341 acaagatcga cccacgcgat tgtcgtctga aggtcggcaa agagatgttt acattgtttg
     8401 ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga tatctttctt gacctgaaat
     8461 tccacgatat tcctaacact gcagcgcacg ctgtcgctgc tgcagctgac ttaggcgtgt
     8521 ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat gaccgcagcg cgtgaggcac
     8581 tggttccgtt tggcaaagat gcaccgcttt tgattgctgt aacagtgttg accagcatgg
     8641 aagctagcga cctggccgat cttggcgtga cactgtcacc tgcagattat gcagaacgtc
     8701 tggcggcact gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct caggaagctg
     8761 tgcgctttaa acaggtattc ggtcaggagt tcaaactggt tacaccgggc attcgtccgc
     8821 aggggagtga tgctggtgac cagcgccgca ttatgacgcc agaacaggcg ttggcggctg
     8881 gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca gcgcaaacac
     8941 tgaaagcgat caacgcctct ttacagcgga gtgcatgatg agtgattcca acagccgtct
     9001 ggtctactca acggaaaccg gacgtattga tgagccaaaa gcggccccag tacgccctaa
     9061 aggcgatggt gtggtgcgta ttcagcgtca gaccagtgga cgtaaaggta agggcgtttg
     9121 cctgattacc ggtatcgatc tcgatgatgc cgaactgaca aaacttgcag cggaactgaa
     9181 gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt attgaaatcc agggcgataa
     9241 gcgtgattta ttaaaatcac tacttgaagc gaaagggatg aaggtaaaac tcgcaggcgg
     9301 ttaacataaa aagccacgga tatatccgtg gctttcgaat attttactgt gtgtattatt
     9361 cagttatttc gtactgaaca aaatcaagcg tattataatt gcgagtcgat aatcgacatt
     9421 tatttacgat tatttaccga cctggtgacc aataacacca ccgacagctg caccacctaa
     9481 tgtacccaac gtactgccat cggtcagtac tgcaccgcct aatgcccctg cacccgcgcc
     9541 gattgcggtg ttgcggtccc gtttagacca gttagaacag gcactcagag acattgccaa
     9601 agtaattgcc agaacagccg cggtcatttt tttgctcgtt acaaacataa tactctctcc
     9661 tgaatttatg attcacggaa gtaagctctc tataactata atagctaaga ataagcctgg
     9721 tgaaattatt ccgtgaaatc tgctcgcgcg cagggttata tcacgcaggt gataatgact
     9781 tcctgttata tcgctgataa taattttata tcttaagcgt gttaataaca ggtaaatagt
     9841 cttaattatc aaccaggaat catcttagag cggatgattt gccaaactgc aaatcatccg
     9901 tagagaaggg aaatggttaa atgtcaacga cgtgaacggt gagtttactg tgctcaagat
     9961 gcatcagatc gctggcgcgt attttcgaat cggtaatcac gcgattgaat cgctcaacgg
    10021 gaccgatgga gtatgaatgt acagcaccaa atttcgagct gtcagtcagg actatcactt
    10081 cgcactcttt ttccagcacg gcattgacca catcggtacg catcatatcg cgaccggtga
    10141 atccagtttc aggttgccag ccatcaatac caataaatgc tttgctgaaa tgcacctgct
    10201 ggatgcactg gcgtgtcaac gggccaacca tactttcgct ttttttctgg tacacgccac
    10261 cgagcaaaat gacttcacaa ggcgcgtctt tcagcaaatg cgcgatgtag ctgctgaccg
    10321 tgatgatagt gacatttttc ttctgctccc ccagagtccg ggcgagcagg gcattgctgc
    10381 tgccattttc gataaagatg gtttcgcccg gttgaaccag tgacgcggca aactcggcga
    10441 gttcacgctt cagcgtatag ttgctcatca tacgggtttc gacgtcatca ctatcaagcg
    10501 aaactgcaaa gccatgtgca cggcggaggt aactcagttt ttcgagggtg ttgagatcct
    10561 ggcgaatggt aacttcagaa actccagtgg cttttgccag atcggttacg ctaacctgac
    10621 cctggtcaat gaccatctgt agaatagttt gttgtcggga attcatcgct gtaattctta
    10681 taacgttata atactaaata aaaaaatact gacgcgccgg gcatcctcaa ataggaaaac
    10741 ctgaaggcac gattgttaaa tctcccacgg tgccttcggg atgtctggcg tagattcagc
    10801 aacctggcgc aagagttctg ctttaagtat ctcgagatta tggatcgcag agtggtaatc
    10861 gcctgccaca aggatatcta acacaatatc aatacgttcg gcaacgcgct gggcatcaaa
    10921 ttcagacatc aaggacatcc ttatagcaaa agtggaaaat gaataatgca aaaattgccg
    10981 ctaaaagaga agtgtttaac agcaacggct aattatcatc caggaatacg atatataatg
    11041 acgggatata gcgctaagta tatatattca tctacttatg cgcgcttcag atagcgttta
    11101 taccagcgtt cgaaggcgac ggcgggcatc ggtttggcaa acaaaaatcc ttgccgctca
    11161 ttgatcccgt tcttggttaa aaaagcatct tcctttgcac tctctacacc ttcggcgatc
    11221 acctgaagat tcaatgcctg ggccacagcg acgatcgccc ggaccagtga ctgcgagaca
    11281 ggttgtttgt gaatatctcg aacaaaaacc tggtcaagtt tgatggcatc gatcggaaag
    11341 cgcgccagtt gcgaaagtga agagtagccg gtaccaaaat catccagatg cacttgcgca
    11401 cctagttggc taaattgttg aataacagac agtgccagtt catcattctc aatcagacaa
    11461 ctctctgtca gttcaacatc tatagggcag tattcaaaat tgagttcctg gagaacctgt
    11521 ttcagggcgg tgaaaatggt ttgatcggcg agctgacgtg cagaaatatt taccgccact
    11581 cgcaggttta tgcctttatc ccgccacttt gccacctggc gtacgacatc gagaatcacc
    11641 caacggccta aaggcacaat tagccctgac tcttcggcgt aggaaatgaa gtccaacggt
    11701 ggaatcaacc cacgttcagg tgactgccaa cgtactagtg cttccagact gcgcacttcg
    11761 ccacgccagg tgattttcgg ttgatagtga ataaccaact gatcgttttc cagtgcttta
    11821 cgcaagttgg tatccagcca gagatattca aatacccgtt gattcatttc tggggtaaaa
    11881 acgcaaaatt gtcctcgtcc gccttccttc gctgtgtaca ttgctgtgtc ggcgtgacga
    11941 ataatagccg tgctgtctga accatgttcg ggagagagtg caatacctac tgaacagctg
    12001 gtataaactt caattaaacc aatgcgaaag gggagccgta agcgggtcaa aattcgtgat
    12061 gccattgctt ccagcgcgct ttgtgaggtg ttggatgcca gtaccagaaa ctcatcccca
    12121 cctggacgcg ccaacacctg gtcatgttcg agacagctta aaatagccaa tgacacgtcg
    12181 cgtaataact ggtcaccaaa caaatgccca taggcgtcgt tgaccttttt gaaattatcc
    12241 aaatcaagat acacaacccc aactttattg ttatctgcat gattaatagc gtgatcgatt
    12301 aaatcctgca ttgcgttacg attcggcagt ccggtgatac tgtcggtatt tgccagaata
    12361 cgcagtcgct cctgagcgcg gcgctcttcg gtaatgtcgg tgccggaaca gattaaaaaa
    12421 atctcgcttt tgccactgcc gctgtggaca aatttattgc gaaacagaaa cagccgctgg
    12481 cctttacatg ttggtatcca cagttcgact tcatatgcat tgccgcttcg aaaaaataca
    12541 cggttattgc gcctggatgc cgcagcttca cgacggctca taaacagttt aaacacgctt
    12601 tggccaatga cgtcgtgttc tttcaaccca gtgtaatctt cacataaccg attgaagcgt
    12661 tggatattcc cccggctgtc gagaatcact attgcggaat gtgcttcaga aactacctgc
    12721 tcagcaaagg aaagcccgtg tgataaatca cgagcaactg ccggtgtgtc atgccatgca
    12781 gacgcgttgc cagcccattc ccgtttattg atttttcgtc ctaccagatg cacagaaaga
    12841 tcgctctcat cgacggtcag actcatcatc aggctggagg tgatgaccgt catttccctg
    12901 atgcgagcag cctgttcggc agataactgc aaagtacgat caggttctgt ggtttcggtg
    12961 gtagaaaagc gcaaaacatc gctgctttcc gtcaaccgcc agtatggatt gtgcgatccg
    13021 agaaagttgt acaacgttgt ggactcccta acggttttca tgatgcatat cctccggtta
    13081 aataaaa
//
LOCUS       DAJTNN010000112        13021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTNN010000112 DAJTNN010000000
VERSION     DAJTNN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.112"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..176
                     /locus_tag="OO150_002820"
     CDS             <1..176
                     /locus_tag="OO150_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5516898.1"
                     /translation="AAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRRL
                     SESVPCMPHKKAG"
     gene            197..898
                     /gene="mlrA"
                     /locus_tag="OO150_002821"
     CDS             197..898
                     /gene="mlrA"
                     /locus_tag="OO150_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR5516899.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDDGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARRKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            958..1065
                     /locus_tag="OO150_002822"
     CDS             958..1065
                     /locus_tag="OO150_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5516900.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1046..1777)
                     /gene="yehW"
                     /locus_tag="OO150_002823"
     CDS             complement(1046..1777)
                     /gene="yehW"
                     /locus_tag="OO150_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5516901.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFVVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1782..2708)
                     /gene="yehX"
                     /locus_tag="OO150_002824"
     CDS             complement(1782..2708)
                     /gene="yehX"
                     /locus_tag="OO150_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5516902.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVCREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(2701..3858)
                     /gene="yehY"
                     /locus_tag="OO150_002825"
     CDS             complement(2701..3858)
                     /gene="yehY"
                     /locus_tag="OO150_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5516903.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3865..4782)
                     /gene="osmF"
                     /locus_tag="OO150_002826"
     CDS             complement(3865..4782)
                     /gene="osmF"
                     /locus_tag="OO150_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5516904.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQISGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5034..7331)
                     /gene="bglX"
                     /locus_tag="OO150_002827"
     CDS             complement(5034..7331)
                     /gene="bglX"
                     /locus_tag="OO150_002827"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5516905.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPFTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGSKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDMVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7527..9242
                     /gene="dld"
                     /locus_tag="OO150_002828"
     CDS             7527..9242
                     /gene="dld"
                     /locus_tag="OO150_002828"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5516906.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPSSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALQRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9281..10213)
                     /gene="pbpG"
                     /locus_tag="OO150_002829"
     CDS             complement(9281..10213)
                     /gene="pbpG"
                     /locus_tag="OO150_002829"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5516907.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10387..10974)
                     /locus_tag="OO150_002830"
     CDS             complement(10387..10974)
                     /locus_tag="OO150_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5516908.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11144..11722
                     /locus_tag="OO150_002831"
     CDS             11144..11722
                     /locus_tag="OO150_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5516909.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(11852..12613)
                     /locus_tag="OO150_002832"
     CDS             complement(11852..12613)
                     /locus_tag="OO150_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5516910.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(12666..>13021)
                     /locus_tag="OO150_002833"
     CDS             complement(12666..>13021)
                     /locus_tag="OO150_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="may be involved in resistance to puromycin,
                     acriflavine and tetraphenylarsonium chloride; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR5516911.1"
                     /translation="DSGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTL
                     AEKNQHQAQIERDTLRVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLD
                     ASIQLTGALGGGYKR"
BASE COUNT         3136 a         3648 c         3285 g         2952 t
ORIGIN      
        1 ttgccgcagg agctaatcct agttttatcg caagccagat gggccacaca aacgcacaaa
       61 tggtattcaa tgtttacgga gcatggatga aagacaacaa tcacgaacag atagaacttc
      121 ttaacagaag actatctgaa agtgtcccat gcatgcccca taagaaagcg gggtaaaata
      181 aaaacttgca aaatcaattg gtttaccttt aatccctgtc acgttacgcg cgtggcagag
      241 gcgttacggg ttgctgaaac cgcaacggac agacgacggt catcgactgt tcaacgatgc
      301 cgatattgac cggatccgcg agatcaaacg ctggatcgac aacggcgtgc aggtcagcaa
      361 agttaaaatg ctgctcagta atgaaaatgt tgatgtgcag aacggctggc gcgatcagca
      421 agaaacatta ctgacctacc tgcaaagcgg caatctgcat agcctgcgaa cgtggatcaa
      481 agagcgcggt caggattacc ccgcccagac actcaccaca catctgttta ttcctctgcg
      541 ccgacggctt cagtgccaac aaccgactct ccaggcgctg ctggcgatcc tcgacggcgt
      601 actgatcaac tacatcgcca tttgtctggc ttcggcacgt agaaaacagg gtaaagatgc
      661 gctggtggtt ggctggaata ttcaggatac cacccgtctg tggctggagg gctggattgc
      721 cagtcaacaa ggatggcgca ttgatgtcct cgcccactcg ctcaatcaac tacgccctga
      781 actattcgaa ggccgtacat tgctggtgtg gtgcggtgaa aatcgaacct ccgcccaaca
      841 gcagcaactc accagttggc aagaacaagg ccatgatatt ttcccactcg gcatttaatg
      901 attcgttaac aaatgcgctt tactgtacaa tcctttcgtt aacataagga gtgcaatatg
      961 cgcatagcta aaattggggt catcgccctg ttcctgttta tggcgttagg cggaattggt
     1021 ggcgtcatgc tcgcaggtta tacctttatt ttgcgtgctg gctaagcgcc tgcaccagcc
     1081 tttcaaacag gcggtctgca atgatcgccg ccagtgccac cagtaacgcc ccctggatca
     1141 catacgcggt attaaatccg ctaagcccga tgatgatggg cgtacccagc gtgctggccc
     1201 ctaccgttga ggcgatcgtc gccgtaccaa tgttgataat caccgaagtt cgcacgcccg
     1261 ccagaatcac cggagccgcc agcggtagct cgaccttacg cagtcgctga ccacgactca
     1321 ttcccatacc tttcgcaact tctgtcacgc tggcatcaat cgctcccagc ccggcaagtg
     1381 tcgcctgcag gacgggcagc acaccgtaaa ggatcaaggc gataatcgct ggttgcagac
     1441 caaagccgat caccggaacg gcaatcgcca gcactgcgac aggcggaaaa gtctgtccaa
     1501 cggcggcaat agtttccacc agtgggcgaa attccgcgcc ccacgggcga gtgacagcaa
     1561 ttccggcacc agtgccaatg atcaccacaa acaaactcga aattcccacc agccagaaat
     1621 gagccagtgc cagagctgca aaactttctt gctgataaac gggtcgtggc agttgtggga
     1681 acaaggcagc aaacagcggc tggctgtaag gcagccagaa aatcagtgcc acaaacaaag
     1741 caatgagcca gaacagcgga tcgcgcaaca tcttcatacg cttacgcctc ctccagcaga
     1801 tcctgaaaat gcagcgtgcc gcaaggctgg ccctgcgtgt tcaccaccgg cagcacctcg
     1861 catccccgcg cgacaaacag ggagagcgca tcgcgtagcg tcatctcttc tgccagtgcc
     1921 tcaccttctg cccgttcttc gcgacacacg taatccgcca cactacgtaa cgaaagcagg
     1981 cgcacaccca gttcgctgcg cccaaaaaac tggcggacaa aatcgtttgc cggacgagtc
     2041 agcatcgtca gcggattgcc ctgctgcacc acttcaccgt gatccatcaa taccagatgt
     2101 tctgccagcc gtagcgcctc atcaatatca tgagtgacca gcacaatggt acgccccagc
     2161 aaacggtgaa tgcgcgtcat ctcttgttgc aacgcgccgc gcgttaccgg gtccagtgcg
     2221 ccaaaaggtt catccatcag taagacttgc ggatcggcag ccagtgcgcg cgccactccc
     2281 acacgttgct gctgaccacc ggaaagctga tgcggataac gctcacgtaa atttgactcc
     2341 agccccagta gcgccattaa ttcgtcgata cgatcgtcaa tccgcgcccg cgaccatttt
     2401 tgtaattgcg gcacggtagc gatgttttgc gccacgctcc agtgggggaa caggccaata
     2461 gattgaatag catagcccat ccggcggcgc aactccagta ctggcagcga gcgaatttct
     2521 tctccggcaa agcggatcac tccgctgtca tgctccacca ggcggttaat cattttcagg
     2581 gtggtggatt tgccggagcc agatgtgcca atcagcaccg aaaaactccc ttcctgaaaa
     2641 ttgagattga gatcgttaac ggctttttgt gcgccgaaca gtttgctgac atggctaaat
     2701 tcaatcatta cgtttcacct tcagcagtgc gataagcaaa tcgaacagcg cgtcggtaag
     2761 caccgccaga acaattaccg ggatcacccc cagcaacact aaatcaatgg cgctgcttag
     2821 cagcccctgg aaaaccagcg caccaaaacc gcctgcgccg attaacgccg caatcaccgc
     2881 catacctaca gtttgcacca tcaccacccg caggctgcgc agaaataccg gtaacgccag
     2941 tggtaactga acatgcagga atcgctgcgc cccgctcatg cccatcgctc tggcactctc
     3001 cagcacatcg cgcgggatct ggttcaaacc gactaccacg ccgcgcacca gcggcagcaa
     3061 ggcatagagc accagcgcaa tcagtgcggg tgtcattccg gttcctgcta tgccgagctt
     3121 ccccagccac ggaaaggccg tcaccagcgc ggcaagcggc gcaatcaaca ggccaaagag
     3181 cgccaccgaa ggcacggtct gaatgacatt gagcagagaa aaaattgccc cctgccgagc
     3241 agtggaaaag tagcaccaga tgcccaacgg cacaccaatc actaacgcag gcagcaccgc
     3301 accaaacagc aacgtcagat gttgtgccag cgcgtcgtca aacacatcct gacggttggc
     3361 gtattctttc attagtgaga gatcgttaag cgtgccggag tacagcaacc acagcggaat
     3421 aatggcaatc tgcatatgca acaaccagcg ccacagcgga tgcgtggaga ttcggcggat
     3481 ggcatcgcta caggccagca atgccagcgc cgcagccagc cagaaaccac tgccgaggct
     3541 ggtacgcgcc agcgcactgc cattttgcgc cagttgtgtc gccgcctttc ccgcgcccca
     3601 caccagcaat acgaagacga attgcgccag aatgagtgca caaatgctgc cttttttacc
     3661 gggaataaaa caggccgtca gccaggcgca accaacgccc accagcatcc agatcgtttg
     3721 cggccacagc tgccagagat gacgcccctc acccgaaact aaacgattag gcgcgtaact
     3781 gataaacggc agcgccgctg cgattgccgt cagcaacagc agcagcgcca gaacaggatt
     3841 aatacggaaa taagtcacgg gaaattactt cgtccaccct ttttgtttca ggtagtcggc
     3901 agccactttt ttggcatcca gtccttccac agcaatgctg gcattcagtt gctgcaatgt
     3961 ttttgcatcg aggctggcga agactggctg tagccactgt gccatttgcg gatactcctt
     4021 caacaccgac tcacgcacca ctggtgcagg cgcgtagata ggttgcacac cttgcggatc
     4081 gcttaaggtt tgcagcccca gcgccgctac cgggccgtca gtgccgtaag ccattgcagc
     4141 attaacgcca gagatttgct gggcagcggc tttgatcgtc accgccgtgt cgccaccagc
     4201 cagtgacagc aactgatcct gaccgagctt aaagccatag gctttttcaa acgcgggtaa
     4261 cgcatcggcg cgttcgataa actctgccga ggctgccagt ttgaaggtgc cgccctcttt
     4321 cagataacga ctcaggtcgg caagcgaagt gagtttattt ttctctgcca catcctgacg
     4381 cacggcgata gtccaggtgt tatttgcagg cgcgggcgtc agccagatta acttgttgtg
     4441 ctccgaatcg agctttttga ctttctcgta accttgctgc gcgtttttcc acgctgcatc
     4501 gttttcatct ttaaagaaga aagcgccatt gccggtatat tccggataga tatccagttc
     4561 accggaagta atcgccccgc gcaccacagg agtcgttccg agttgcactt tattcaccgt
     4621 cggtacgccg tggctttcca gcacctgcaa gatgatgttg ccgagtagcg cgccttcggt
     4681 atcgattttt gaaccgactt taacggggga agccgcctgt agcggcaggc tcacggctgc
     4741 caacataacc agtgaacctg cccagacctt tgagagtggc atgatgcttt cctcattctt
     4801 ttactgttgt tttcagcgaa ttaagagaaa agcatagttg ataatgacag agttagcctg
     4861 agcggaagga ttcagttgca gaatcagata aatacttaaa gggcatattt tgcccggcgt
     4921 ttcttgccgg atgcggcgta aacgccttat ccggcctaca aaggtcgaaa attcagcaag
     4981 ttaagatatg aacatcaggc aaagggcgca aacgtcgcgc ccttactaaa gcattacagc
     5041 aactcaaact cgcctttctt aacgcgtgcg gaatcagtgc cgataaagac attgaacttg
     5101 ccaggctcgg cgtcatattt catctgttga ttccagaact tcagcgcctc gatatcaatc
     5161 gggaagctga cagtctgagt ttcgcccggt ttcagggtga ttttctcaaa gcctttcagc
     5221 tgcttcactg gacggctcat ggaagccgtc acatcctgca agtacatctg tactaccgtg
     5281 gccccttcgc gtttaccggt gttcgtcacc tgcacgctgg ccgtcacttt gccgtcacgc
     5341 ttcatggtcg gcgcagaaag tttcacatca gagacggtga aagtggtata gctcagacca
     5401 tagccgaacg gataaagcgc cccgttagct tcatcaaaat aacgcgaagt gtatttgttc
     5461 ggcttgtcgg cattatacgg acgaccggtg ttcagatggc tgtagtacac cgggatctgc
     5521 ccgacagaac gcgggaagga catcggcagc ttgccggacg ggttgtaatc gccaaacaac
     5581 acatcggcaa ttgcattacc gccttcagtc cccgcaaacc aggtttccag aatcgcatca
     5641 gcctgctgat cttctttcac cagcgccagc ggacgcccgt tcatcagcac cagcaccagc
     5701 ggtttaccgg tggctttcag cgccgcaatc aagtcacgtt ggctttgcgg aatagtgata
     5761 tcggtacggc tggaggcctc gtgcgccatc ccctgtgctt caccgaccac agccaccacc
     5821 atatcagatt gtttcgccgt ctgcaccgct tcatcaatca tctcttgcgg cgagcgcgga
     5881 tcgactttga ccgcttcttc atactgattc aggaaatcga taatgccttt gtcactggta
     5941 acgttcgccc ctttggcata cagcacttta ccgttttcac cgacggcatt tttaatcccg
     6001 gtcagtacgg tcacggattg atcggcaaca ccggctgcgg accagctgcc catcacgtcg
     6061 cgtttactgt ccgccagtgg cccaaccacc gcaatggtgg ccgatttttt cagcggtaac
     6121 gtttcgagac ggtttttcag caacaccagg ctttcgcgcg ccacttcacg tgcttcttta
     6181 cggtgcaggc ggctttcggc attggtatcc accgggtcag actctttcga ccccaaatgg
     6241 ctgtatgggt cgttaaacaa tcccatatca tatttaacgt tcagtacatg gcgggcagca
     6301 tcgtccagct cttccatcgt cactttgccg gatttgatca acccaggcag atacttcgag
     6361 taatactcgt cgctcatact catgttgatt ccggatttca gcgccacgcg caccgcatct
     6421 tccgggtctg ccgccgtgcc atgtttaatc agctctttga ttgcaccgtg atcggaaacg
     6481 gtgatgcctt taaagcccca ctggtcgcgc agaacatctt tcagcagcca ggaatcggag
     6541 gtggctggcg tgccgttcag cgagttcagc gccaccatca ccgcgccgct gcctgcgtcc
     6601 agccccgctt tgtacggcgg catataatca ttaaacaggc gctgcggact catatcgacg
     6661 gtgttgtact ctttaccgcc ttctaccgcg ccgtatgcgg caaagtgttt gacgctggtc
     6721 atcaccgagt agcgatctgc cgggctttta ccctgcatcg cttccaccat ggttttaccc
     6781 attgttgagg tgagatacgt atcttcgcca aaaccttcgg aagcacgtcc ccagcgcgga
     6841 tcgcgcgaga catcgaccat cggtgcccag gtcatattca ggccatcatc tgccgcttca
     6901 taagcagaga cacgtccgac cgttttcact gcatcgaggt taaaagacga ggccagaccg
     6961 aggctaatcg ggaacaccgt gcgctgaccg tgcagcacgt cgtaagcaaa gaaaagagga
     7021 attttcaggc ggcttaattc catcacctga tcctgcatgg cgcggatatc ctgacgggtt
     7081 acggtgttga aaatcgcccc aacctgaccg tctttgatca tctcgcggat cgcctctttc
     7141 gggttatccg ggccgacgct gattaagcgc agctgaccaa ttttctcatc aactgtcatt
     7201 ttcttaagca attcggtgac gaacgcatcc cgcgcttcgg gcgtgaatgg atggttgccg
     7261 aataaatcat ccgccagtgc tggctgcagg gccagactca ccgcgattcc tactgaacat
     7321 agccatttca tatggatttt tcttcctctg ttgcccgacg aggcagcgaa aatggtgcaa
     7381 aaaccgtagt ttgccataag catgatggag agagaaaaag aatgctcagt ttattgtctg
     7441 aattttcaaa atattcactc gctgaattgt catacaaggc gctattctag tttgtgatat
     7501 tttttcgcca ccacaaggag tggaaaatgt cttccatgac aacaactgat aataaagcct
     7561 ttttgaatga acttgcccgt ctggtcggtc attcacacct gctcaccgat cccgcaaaaa
     7621 cggcccgcta tcgcaagggc ttccgttctg gtcagggcga cgcgctggct gtcgttttcc
     7681 ctagctcact actggaattg tggcgggtgc tgaaagcctg tgtcaccgcc gacaaaatta
     7741 ttctgatgca ggctgccaat acaggcctga ccgaaggatc gacgccaaac ggtaacgact
     7801 atgatcgcga tatcgtgatc atcagcaccc tgcgtctcga caagctgcac gttcttggca
     7861 agggcgaaca ggtgctggcc tatccgggca ccacgctcta ttcgctggaa aaagccctca
     7921 aaccgctggg acgcgaaccg cactcagtga ttggatcatc gtgtataggc gcatcggtca
     7981 tcggcggtat ttgtaacaac tccggcggct cactggtgca acgtggcccg gcgtataccg
     8041 aaatgtcgtt attcgcgcgt ataaatgaag acggcaaact gacgctggtg aaccatctgg
     8101 ggattgatct gggcgaaacg ccggagcaga tccttagcaa gctggatgat gatcgcatca
     8161 aagatgacga tgtgcgtcac gatggtcgtc acgcccacga ttatgactat gtccaccgcg
     8221 ttcgtgatat tgaagccgac acgcccgcac gttataacgc cgatcctgat cggttatttg
     8281 aatcttctgg ttgcgccggg aagctggcgg tctttgcagt acgtctagat accttcgaag
     8341 cggaaaaaaa tcagcaggtg ttttatatcg gcaccaacca gccggaagtg ctgaccgaaa
     8401 tccgccgtca tattctggct aacttcgaaa atctgccggt tgccggggaa tatatgcacc
     8461 gggatatcta cgatattgcg gaaaaatacg gcaaagacac cttcctgatg attgataagt
     8521 taggcaccga caagatgccg ttcttcttta atctcaaggg acgcaccgat gcgatgctgg
     8581 agaaagtgaa attcttccgt ccgcatttta ctgaccgtgc gatgcaaaaa ttcggtcacc
     8641 tgttccccag ccatttaccg ccgcgcatga aaaactggcg cgataaatac gagcatcatc
     8701 tgctgttaaa aatggcgggc gatggcgtcg gtgaagccaa atcgtggctg gtagattatt
     8761 tcaaacaggc cgaaggcgat ttctttgtct gtacgccgga ggaaggcagc aaagcgtttt
     8821 tacaccgttt cgccgctgcg ggcgcagcaa ttcgttatca ggcggtgcat tccgatgaag
     8881 ttgaagacat tctggcgttg gatatcgctc tgcagcgtaa cgacaccgag tggtatgagc
     8941 atttaccgcc ggagatcgac agccagctgg tgcacaagct ctattacggc cattttatgt
     9001 gctatgtctt ccatcaggat tacatagtga aaaaaggcgt ggatgtgcat gcgttgaaag
     9061 aacagatgct ggaactgcta cagcagcgtg gcgcgcaata ccctgccgag cataacgtcg
     9121 gtcatttgta taaagcaccg gagacgttgc agaagtttta tcgcgagaac gatccgacca
     9181 acagtatgaa tccggggatc ggtaaaacca gtaagcggaa aaactggcag gaagtggagt
     9241 aaaaattacg gatggcatag tatcgccatc cgtcattcac ttaatcgttc tgtgccgtct
     9301 gccccgccgc cgccatttgg gcggcttttt gttttttata gctcaacgct gctgctggaa
     9361 caggcatcac tttaccggtt tcaatccagg tacgcaggcg gctggcatcg gcaaaatggg
     9421 tatatttgcc aaacgcatcc atcactacca gcgccaccgg tttattattg ataaccgtac
     9481 gcatcaccag acaatggccc gccgcattgg taaaaccggt tttggttaac tgaatattcc
     9541 agttatcgcg atacaccaga tggttagtat tgcggaacgg cagcgtatac gtcgggttag
     9601 agaaggttgc catatcttcc cgagtagtac ttaactgccc gatcaacgga tattgtttgc
     9661 tggcaatgag cagtttggtt aagtcacggg cagttgaaac gttatgcacc gacaatccgg
     9721 taggttcaac aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca ttcattgcct
     9781 taataaaggc tttgtaacca ccgggataat ggtgtgcaag gcttgccgcc gcgcggtttt
     9841 ctgaagacat cagcgccagc aacagcatat ctttacggct gatttcgcta ttcagtcgta
     9901 cgcgcgaata gacccctttc atctccggcg tctggctgat atccactttt agtttttcat
     9961 ccagcggcag tcgtgcatcc agcacaacca tcgcggtcat taatttgctg atagacgcaa
    10021 tcggacgcac cagatccggg tggttcgagt agatcacttt gttggtattc agatcaacaa
    10081 tcatcgcgct accggaggca atttcaggtt gtgaagcggt gatagcggct gccgttttcg
    10141 caacggcctg cggtgccaaa ggcacagcca gcatcagggc caggctaaat aaagaaactc
    10201 gaaatttcgg catgatgagc attcagatag tggttcacgc gcacaggttg cgcaccgccg
    10261 gagtaaggat ttactgaggc tagcgacgcc atcataacga gcaaaaagtg cgatcgtcaa
    10321 aggagaatcg tgaggaaatg ctgcattgct gacatttacg ccagcaatgc aacgtcaaag
    10381 aactttctag aacaaacgat aaccgtagcc ccatagtata acggttaggg cgagcagcac
    10441 ttccagtacc agcacgccaa tcgccagcgt cgaactagag aagctaaggc cttcctcttt
    10501 gttgatattc aggaagctcg gaataccaag gtaaagcagg tagccggtgt aaaacagcgc
    10561 caccgtgccg accagcgcac acaaccagac cagtggataa agcgccacca aaccgcttaa
    10621 aaacagcgga gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg acggacgctg
    10681 cggataatta cgcgccatcc accagatgac ccgccccatt accgcaaccc cagccagcat
    10741 aacgccataa aacaggacag ccagcgccag tccggtaaac caggataact tcaggatagt
    10801 gccatcgcca aaattccagc caatctgtgt agtgccaatg aaggcgcaaa tcaccggaat
    10861 cgccgccatc agcaaaacgt ggtgggtgta atggtgagaa atcgtttcgt tttcgcgatt
    10921 aatcacctgc atttcacgat cgggatggga aaacagtccc cagacatggc tcataccgcc
    10981 cccttgttgt gagttcatga acctgacagt tcaagtataa gtcagcttgt gattattttt
    11041 tgttcgctca tgtaatttgc accgtctgat gtacgtcctt tttcagcgtg aatgatttcc
    11101 agggtgtatg cttacaggca accaaaggga gacagactgg cctatggatc tcaatacact
    11161 catctcacaa tatggttatg ccgcgctggt gatcggtagc ctggcggaag gtgaaaccgt
    11221 gactttgctg ggaggcgttg cggcacatca ggggctatta aagttcccgc tggtggtact
    11281 ttctgtggcg cttggcggca tgattggcga ccaggtgctt tatctgtgcg ggcggcggtt
    11341 tggcggcaag ctgttacgcc gtttctcgaa acatcaggat aaaattgagc gggcgcagaa
    11401 acttatccaa cgccatccgt atctgtttgt cattggtacg cgctttatgt atggctttcg
    11461 ggtgattggc ccgacgctga ttggtgccag ccagctgccg ccgaaaatct ttctgccgct
    11521 gaatattctc ggcgcatttg cctgggcgtt gatttttacc actattggtt acgctggtgg
    11581 tcaggtgatt gcgccgtggt tgcacaatct cgaccagcat ttgaagcact gggtctggtt
    11641 gattctggtc gtggttctgg tggtgggcgt gcgctggtgg ctgaaacggc gcgggaagaa
    11701 aaagccggat catcaggcgt aaaaccattg ccctgggtaa ggcgttcgcg ccgcatccga
    11761 catccggtgc accatgcctg atgcgacgct tgtgcgtctt atcatgccta ccaaaatttt
    11821 acccattgaa ataaccacgc acctcactgc gctattctgg gttgaactgc ggattcgcca
    11881 acataaagcc gccatccact atcaacgact gcccggtggt gtaatttgcg ccctctgagc
    11941 acagccacgc taccaggctg gcaatctcat gcgttgcgcc aaaacgccgc agaggaatcg
    12001 aaggctccgc gtcgggcttc acgtcgctgt catccatgcc attcattggc gtggcgatcg
    12061 ccccaggcgc gactgcgttc accaaaatct tatgcctgac cagctccagc gccatcgctt
    12121 tggttaaccc accgagcgca tgtttagcgg ctgtgtaggc gctggcatcc ggcagcggcg
    12181 tatgttcatg taccgatgta atgttgatga tgcgaccgcc ctgcccttgt ttcaccatct
    12241 ggcgggccgc aatttgtgag cataaaaatg caccatcgac atcaacggta aaaatcttgc
    12301 gccactcatc gaaagccata tcaagaaacg gcgctttggt cattgcgccc gcattattca
    12361 ccagcacatc aatacgcccc agccgttgaa tgagtttctc cagcgcctgc gcaccttctg
    12421 gcagtttacc gagatccagc tgcacgatct ccgcacgtac gccgtggcta actacctcac
    12481 gcgcggtatc ttttgcccct tcttcatctg agtgccaggt aatgccaata tcaaaccctt
    12541 gctgcgccag taataacgcg cactctttgc cgatccccga atcggaggcg gtaataatcg
    12601 caacctgtgc catcgagttc tccacttaac gctgaataaa cgttaagtat agaaggcgca
    12661 tatcatcagc gtttgtaacc cccgcccaac gcaccagtga gttgaatgga ggcatccagc
    12721 cactgccctt gcaataacag gccattggcc cgctcacgca gcgcggggat tctggcttcg
    12781 ctgacgcggg aaccagcaat gatgcccgcg ttaaagcgcg cctgcgcaag acctaccaca
    12841 cgcaaggtat cgcgctcaat ttgcgcctga tgctggtttt tctccgcaag tgtctgaacc
    12901 tgactggctg cccgcgccac gtcattcacc gcttcaacca ccgctttgtt gtagctggcg
    12961 atagacaagt tgctttcggc ttttgcgata tcgagattgg cgttaagacg accactatcg
    13021 a
//
LOCUS       DAJTNN010000113        13013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTNN010000113 DAJTNN010000000
VERSION     DAJTNN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.113"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..604
                     /gene="mxiE"
                     /locus_tag="OO150_002834"
     CDS             <1..604
                     /gene="mxiE"
                     /locus_tag="OO150_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5516912.1"
                     /translation="NIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALVEHSHAEKCIH
                     FYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLVLYLLRTIEKE
                     KEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVFLHNQTITSAA
                     MNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            591..2291
                     /gene="mxiD"
                     /locus_tag="OO150_002835"
     CDS             591..2291
                     /gene="mxiD"
                     /locus_tag="OO150_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5516913.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSQGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTRNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            2310..3377
                     /gene="mxiC"
                     /locus_tag="OO150_002836"
     CDS             2310..3377
                     /gene="mxiC"
                     /locus_tag="OO150_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5516914.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            3390..5450
                     /gene="mxiA"
                     /locus_tag="OO150_002837"
                     /gene_synonym="virH"
     CDS             3390..5450
                     /gene="mxiA"
                     /locus_tag="OO150_002837"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5516915.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            5463..5864
                     /gene="spa15"
                     /locus_tag="OO150_002838"
                     /gene_synonym="spaK"
     CDS             5463..5864
                     /gene="spa15"
                     /locus_tag="OO150_002838"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5516916.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            5868..7160
                     /gene="spaL"
                     /locus_tag="OO150_002839"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             5868..7160
                     /gene="spaL"
                     /locus_tag="OO150_002839"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5516917.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMATAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELLGETIR"
     gene            7253..7591
                     /gene="spa13"
                     /locus_tag="OO150_002840"
                     /gene_synonym="spaM"
     CDS             7253..7591
                     /gene="spa13"
                     /locus_tag="OO150_002840"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5516918.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            7578..8456
                     /gene="spa32"
                     /locus_tag="OO150_002841"
     CDS             7578..8456
                     /gene="spa32"
                     /locus_tag="OO150_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5516919.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            8450..9331
                     /gene="spa33"
                     /locus_tag="OO150_002842"
                     /gene_synonym="spaO"
     CDS             8450..9331
                     /gene="spa33"
                     /locus_tag="OO150_002842"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5516920.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            9336..9986
                     /gene="spa24"
                     /locus_tag="OO150_002843"
                     /gene_synonym="spaP"
     CDS             9336..9986
                     /gene="spa24"
                     /locus_tag="OO150_002843"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5516921.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            10001..10261
                     /gene="spaQ"
                     /locus_tag="OO150_002844"
     CDS             10001..10261
                     /gene="spaQ"
                     /locus_tag="OO150_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5516922.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            10261..11031
                     /gene="spa29"
                     /locus_tag="OO150_002845"
                     /gene_synonym="spaR"
     CDS             10261..11031
                     /gene="spa29"
                     /locus_tag="OO150_002845"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5516923.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            11038..12066
                     /gene="spa40"
                     /locus_tag="OO150_002846"
                     /gene_synonym="spaS"
     CDS             11038..12066
                     /gene="spa40"
                     /locus_tag="OO150_002846"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5516924.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            12079..12321
                     /locus_tag="OO150_002847"
     CDS             12079..12321
                     /locus_tag="OO150_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516925.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         4177 a         1847 c         2539 g         4450 t
ORIGIN      
        1 aaatataaaa ttcagtgata atgtgaacta tcattatcgc tttaacatta atagttgtgc
       61 aaaatttctt gctttttggg actatttcag tggtgctcta gtagagcata gccatgctga
      121 aaaatgtatt catttctatc atgagaatga tcttcgggat agttgtaata ccgaatcgat
      181 gcttgataag ttaatgttaa gattcatctt ttcatctgac cagaatgtaa gtaatgcact
      241 ggctatgata cgaatgactg agtcatatca tttggttctt tatcttctga gaactattga
      301 gaaagaaaag gaggtaagaa taaagagtct gacagagcat tatggtgtgt cggaggctta
      361 ttttagatct ttatgtagaa aagctttagg ggcaaaagtc aaagaacaac tgaacacttg
      421 gcggttagta aatggtttat tagatgtatt tttgcacaat caaacaatca cttctgctgc
      481 catgaataat gggtatgcat caacatccca tttttcaaat gagataaaaa cacgactagg
      541 cttttctgca agagaattat cgaatattac ttttttagtg aaaaaaataa atgaaaaaat
      601 ttaatattaa atctttgact ctcttgattg tattgttacc attgatagtt aatgctaata
      661 atattgactc tcatttatta gaacaaaatg acatagcgaa atatgttgct caaagtgata
      721 cggtaggatc tttttttgaa cgtttttctg cgttattaaa ttatcctatt gttgttagca
      781 aacaagctgc aaaaaagaga atatctgggg agtttgattt atctaatcca gaagaaatgc
      841 ttgaaaaaat gacattactt gtgggactca tatggtataa agatggaaat gcattataca
      901 tatatgactc tggagaatta ataagcaaag ttattttgct ggagaatata tcacttaatt
      961 atcttattca gtatctgaag gatgctaacc tttatgatca cagatatcct ataagaggaa
     1021 acattagtga taaaacattc tatatatctg gtcctccggc attggttgaa ctggtagcta
     1081 atacagcaac tttgcttgat aagcaggttt cttctattgg gacagataaa gttaattttg
     1141 gggttataaa attaaaaaat acatttgtta gtgacagaac ttataatatg agaggggagg
     1201 atattgtcat cccaggggtg gctactgttg tagaacgttt attaaataat ggtaaggctc
     1261 tgagtaacag acaagccaaa aatgatccga tgccaccctt taatattaca cagaaagtat
     1321 ctgaggatag taatgacttt tcatttagtt cagttaccaa tagttctata ttagaggatg
     1381 tatcacttat tgcatatcca gaaaccaatt ctatattagt caaaggtaat gatcagcaaa
     1441 tacagattat tcgcgatatt atcactcagt tagatgtagc caaacgacat atagaattat
     1501 cattatggat aatcgatatt gataaatctg aattaaataa ccttggcgtt aattggcaag
     1561 gaacggcttc atttggtgat tcgtttggtg catcatttaa tatgagctca tcagcatcga
     1621 taagcacttt ggacggcaat aagtttattg cttcagttat ggcattaaat cagaaaaaga
     1681 aagcaaatgt agtttcaagg ccggtgattt tgacacaaga gaatattccg gctatatttg
     1741 ataataacag gactttttat gtgtcattag ttggtgagcg caattcttcg ttggagcatg
     1801 ttacgtatgg aacattgata aatgttatac ctcgcttttc atctcaggga caaattgaga
     1861 tgtctttaac gatagaggat ggaacaggaa atagtcagtc aaattacaat tataataatg
     1921 aaaatacatc tgtccttcca gaagttggaa ggaccaaaat cagtacaata gcgagagttc
     1981 cacaaggcaa aagtttgttg ataggtggtt atacgcatga aacgaactct aacgaaatag
     2041 tatctattcc ttttctctcc tctattcctg taatcggtaa tgtgttcaag tataaaacaa
     2101 ggaatatcag taatattgta agggtttttc tgattcaacc ccgtgagata aaggagtcaa
     2161 gttattataa tactgcagaa tataagagcc tcatatctga aagagaaata caaaagacta
     2221 cacaaataat cccatctgaa actactttgc tggaagacga aaaatcattg gtttcatact
     2281 taaattacta actataaagt aggtgatgta tgcttgatgt taaaaataca ggagttttta
     2341 gctctgcatt cattgataag ttgaatgcaa tgacaaattc agatgatgga gatgagactg
     2401 ctgatgcaga gcttgattct ggcttggcta atagcaagta tattgactca tctgatgaga
     2461 tggcttccgc tctttcgtca tttataaaca gaagagacct tgagaaactg aaaggaacaa
     2521 atagtgatag tcaggaacgt attttagatg gggaagaaga tgaaattaat cacaagattt
     2581 ttgatttaaa gagaacttta aaagataacc ttcctctaga tcaggatttt atagacagac
     2641 taaagagata ttttaaagat ccaagtgatc aagtcttagc attaagggaa cttttgaatg
     2701 aaaaagatct tactgctgaa caagtcgaat cattaactaa aattattaat gagataatat
     2761 caggtagtga aaaaagtgtt aatgctggaa taaattcagc tatacaggct aaattatttg
     2821 gcaacaaaat gaaacttgaa ccacagcttt tgcgtgcatg ttatcgtggt tttatcatgg
     2881 ggaacatatc aacaacagat cagtatatag aatggcttgg taattttggt tttaatcaca
     2941 gacatacaat tgtgaatttt gtagagcagt cactgattgt agacatggat tctgagaaac
     3001 cgagctgtaa tgcttatgag tttggttttg tgttatctaa attaattgca attaagatga
     3061 ttagaacttc agatgtaatt tttatgaaga aactggaatc ctcaagcttg ctaaaagatg
     3121 gcagtttaag tgcagagcag ctattgctaa ctttattata tatttttcaa tatccaagtg
     3181 aaagtgagca aattcttact tctgttatag acgtatcacg agccagtcat gaggattctg
     3241 tagtgtatca aacatatcta tcttctgtta atgaaagtcc tcatgatata tttaaaagtg
     3301 aaagtgaaag agaaattgcg atcaatattc tacgagagct tgtcacaagt gcatacaaga
     3361 aagagctttc tagataacag gagatgaaag tgatccagtc ttttcttaag caagtaagta
     3421 ctaagcctga attaattata ttagtgctta tggttatgat cattgctatg ttgatcatac
     3481 cattacctac ataccttgtt gattttttaa tagggcttaa tattgtactt gctattctcg
     3541 tttttatggg atcattttat attgaaagaa tattgagttt ttctacattc ccttcggttt
     3601 tgttgataac gacattattt cgtctcgctt tatcaataag tactagtcga ctaattttag
     3661 ttgatgcgga tgcgggtaaa attattacta catttggcca gtttgtcatt ggtgatagtc
     3721 tggctgtagg ttttgttatt ttttcaatag taactgttgt gcagttcatt gttataacaa
     3781 aaggatctga aagggttgcg gaggtagctg ctcgcttttc tcttgacggt atgccgggga
     3841 aacaaatgag cattgatgcg gatttaaagg ctggaattat tgatgccgca ggagctaaag
     3901 aaagacgcag tattctggaa cgtgagagtc aattgtatgg ttcatttgat ggagcaatga
     3961 agttcattaa aggtgatgca attgctggca tcattattat ttttgtcaat ttaataggag
     4021 gtatttctgt tggtatgagt caacatggaa tgtctctatc tggtgcatta tctacttata
     4081 ccatactaac tattggtgac ggattggtct ctcagattcc tgctctgtta atttctataa
     4141 gtgcaggatt tatcgttaca agagttaatg gtgatagcga taatatggga cgtaacataa
     4201 tgtcccagat atttgggaat ccttttgttc tcattgttac atcagctctt gccttggcga
     4261 tagggatgtt gccaggtttt ccgttttttg tttttttcct gatagcagtt actttgacgg
     4321 ctttattcta ttataaaaag gtcgtagaaa aagaaaaaag tctgtctgag tctgattcta
     4381 gtggctatac tggtacattt gatattgata atagccatga ctcatctttg gcaatgatag
     4441 aaaatctgga tgctattagt tcagaaaccg ttcctttaat tttattattt gccgaaaata
     4501 agataagtgc aaatgatatg gaaggtctta ttgaaaggat aagaagtcag ttctttattg
     4561 attatggtgt caggcttcca actattttat atagaacaag caatgagctt aaggtcgatg
     4621 atattgtttt gttaataaat gaggtgcgtg ccgatagttt taatatatac tttgacaaag
     4681 tgtgtattac agatgaaaat ggagatatag atgctctagg tattcctgtt gtttcaactt
     4741 catataatga acgtgttatt tcttgggtag acgtttcata tacagaaaat ctaactaata
     4801 ttgatgctaa aattaaaagt gctcaagatg aattttatca ccagttgtca caagctttat
     4861 taaacaacat aaatgagatt tttggtatac aagaaacaaa aaatatgtta gatcagtttg
     4921 aaaatcggta tcctgatcta ttaaaggaag tcttccgaca tgtgactata cagagaattt
     4981 ctgaggtatt acaaagattg cttggagaaa atatttctgt tcgcaattta aaacttatta
     5041 tggagtcttt ggcgctttgg gctcccagag aaaaagatgt cataacatta gttgaacatg
     5101 tccgtgcatc actttctagg tatatttgta gtaaaatagc tgtttctggt gagattaaag
     5161 ttgtgatgct ttccggatat attgaggatg caataagaaa agggataagg caaacctctg
     5221 gtggctcttt cttgaatatg gatatagagg tttcggatga ggtaatggaa actttagcac
     5281 atgctttgag agaattgaga aatgcaaaaa aaaatttcgt tcttttggta tcagtagata
     5341 tacgtaggtt tgttaaaaga cttatagata acagatttaa gagtatactc gttatatcgt
     5401 atgctgagat tgatgaagca tataccatta atgtattaaa gactatttag tgaggtttaa
     5461 atatgagtaa cattaattta gttcaattag ttagagatag tcttttcacg attggttgtc
     5521 ctccatcaat tattacagat ttagacagcc attcagcgat taccatttct ttggatagta
     5581 tgcctgctat taatatagct cttgtcaatg agcaggttat gttatgggct aatttcgatg
     5641 caccaagtga tgtaaaactt cagtcatctg cttataacat attaaattta atgttaatga
     5701 actttagtta tagcattaat gagctggtag agcttcatcg ttctgatgag tacttacagt
     5761 taagagttgt tattaaagat gactacgttc acgatgggat tgtttttgca gagattctgc
     5821 atgagtttta tcagaggatg gaaatcttaa atgaggtctt ataatcaatg agctatataa
     5881 aattgctcac tcaattatct tttcctaata gaatctcggg gccaatcttg gaaacaagtc
     5941 ttagcgatgt ttcgattggt gagatttgta acattcaggc tggaattgaa agtaatgaaa
     6001 ttattgcaag agctcaggtt gtaggatttc atgatgaaaa aacaatatta agcttgattg
     6061 gaaattctcg tggactttca aggcaaacgt tgattaagcc cactgcccag tttcttcata
     6121 cgcaagtcgg ccgtggatta ttgggagcag tagtcaatcc tttaggggag gttactgata
     6181 agtttgctgt tacggataac agtgaaattc tttatcgacc tgtagataat gctcctccgc
     6241 tatatagtga aagggctgca attgagaagc cttttttaac aggtgttaag gttattgatt
     6301 ctttactcac gtgtggtgaa ggacagcgaa tggggatttt tgcgtcagct ggttgtggca
     6361 aaacttttct catgaatatg ctcattgaac atagtggtgc tgatatatat gttattgggt
     6421 taattggtga gcgaggtcga gaggttacag aaacggttga ttatttgaag aactctgaga
     6481 aaaaaagcag gtgtgtttta gtatatgcaa cttcggatta ctcttcggtt gatcgttgta
     6541 atgctgcata tatagccact gctatagccg aattttttag gactgaagga cataaagtag
     6601 cgctttttat tgattcatta acaaggtatg ccagagcatt acgtgatgtg gccttagccg
     6661 ctggagaatc acctgccaga agaggctatc cggtttcggt ttttgatagc ttacccagac
     6721 ttcttgaaag gccaggaaag ttaaaggcag gtggctctat tactgcattt tacactgttc
     6781 ttttggagga tgatgatttt gctgatccat tagctgaaga ggtaagatcc attttagatg
     6841 gacatatata tttgagcaga aatctagccc aaaaaggaca atttcctgca attgattcct
     6901 taaaaagtat aagcagggta tttacacagg ttgttgatga aaaacatcgt attatggcca
     6961 ctgcatttcg ggagctactt tctgaaatag aagagctaag gacaattatt gactttggtg
     7021 aatacaaacc gggggagaat gcctctcagg ataaaatcta caacaaaata tctgttgttg
     7081 agagttttct gaagcaagat tatcgactcg gttttactta tgagcagaca atggagcttc
     7141 ttggtgaaac aattagataa ggttttaaaa atcaaagata aatatcaacg gtctgttaaa
     7201 ttaattgaag cacacatatt aacactaaaa aaaaaactct ttgtatcgag atgtggaggc
     7261 attagataaa aggattatat acttcctgca actggagaat gacttggaac ctgtaggcgc
     7321 tcagagtgtt agtcagcttt tcaatacaag acgtaagatc gctatagtaa aaaagcatat
     7381 tatccaatat cagtctgaac gaattcttct aaagggaaga attgaagaaa ttcaaaaaga
     7441 tattgatgag gcaaatgctt ctaaaagaaa actgcttcat aaagagagta aaatatgtaa
     7501 acggattggc ttgattaaaa gaaataactt tgccaaacag ttgatacttg atgagttatc
     7561 acaagaggat atgaagtatg gcattagata atataaacct aaattttagt agtgacaaac
     7621 agatagaaaa atgtgagaaa ctatcttcaa tagataatat tgatagtctc gttttgaaga
     7681 agaagaggaa ggttgaaatt cctgagtact ctttaattgc atctaactat tttactattg
     7741 ataagcactt tgaacataag catgataaag gagaaattta tagtggcatt aaaaatgcgt
     7801 tcgaacttag aaacgaacga gcgacatatt ctgatattcc agaatcaatg gccattaaag
     7861 aaaatatttt gataccagat caagatatca aagcaaggga aaaaataaat atcggcgata
     7921 tgagggggat cttttcatat aataagagtg gaaatgcaga caagaacttc gaaagaagtc
     7981 atacttcttc tgtaaaccct gataatctgc tagaatctga taatagaaat ggtcaaattg
     8041 gtttaaaaaa tcatagcttg tctattgata agaatattgc tgacatcatt tctttactaa
     8101 atggaagtgt tgctaaatca tttgagctgc ctgtaatgaa taaaaatact gcagacataa
     8161 ccccatccat gtcattgcaa gaaaaatcaa tagttgaaaa tgataaaaat gtttttcaaa
     8221 aaaatagtga aatgacttac cactttaaac agtggggggc tggacattct gttagtattt
     8281 cagtggagtc tggttctttt gttctaaaac cgtcagatca atttgtagga aataaacttg
     8341 acttaatttt gaaacaagat gctgagggta attacagatt tgatagcagt caacataata
     8401 aggggaataa aaataatagt acaggatata atgaacagag tgaagaagaa tgctaagaat
     8461 taaacatttt gacgctaacg aaaaactaca gattttatat gcaaagcaac tctgcgagcg
     8521 tttttcgatt cagacattca aaaataaatt tacaggcagt gaaagtttag tcactcttac
     8581 ttccgtgtgt ggggattggg taattcgtat tgatacatta tcttttttga aaaaaaaata
     8641 cgaggtattt tcaggatttt ctacacaaga atctttactg catttatcaa aatgtgtctt
     8701 tatagagtcg tcatctgtat tttcgattcc agaactgtct gataagatta ctttccggat
     8761 cacgaatgaa atccagtatg caactactgg gagtcattta tgctgttttt cctcttcttt
     8821 aggtattatt tattttgaca agatgccggt attacgtaat caagtttctc ttgacttatt
     8881 gcatcatctt ttagagtttt gcttaggttc atctaatgta aggctggcta ctttaaaaag
     8941 aattcgtact ggtgatataa tcatagttca gaaactttat aatttattat tgtgtaatca
     9001 agttattatt ggggattata ttgtgaatga taataatgag gcaaaaatta atctgtcaga
     9061 aagtaatggt gagtcagatc acacagaagt ttctttggca ttattcaatt atgatgatat
     9121 caatgtaaaa gtggacttta tccttttaga aaaaaagatg acaatcaatg aactaaaaat
     9181 gtatgtagaa aacgaattat tcaagtttcc cgatgacata gttaaacatg taaatattaa
     9241 agtaaatggt tctttggttg ggcatgggga acttgtttct attgaggatg gttatggtat
     9301 cgagattagt tcttggatgg taaaggagta atctcatgct gagtgatatg tccctcatcg
     9361 ctacactttc tttttttacg ctattaccat ttttagttgc tgctggtact tgttatataa
     9421 agttttctat tgtttttgta atggtacgaa atgcacttgg tttgcagcag gttccatcaa
     9481 atatgactct caatggtatt gcattgatta tggctttgtt cgtgatgaaa cccattattg
     9541 aagctggtta tgaaaattat ctaaatggcc cgcaaaaatt tgatacaata tcagatattg
     9601 tccgtttttc tgattctggc ttgattgaat acaagcaata cttgaaaaag catacagatc
     9661 ttgagttggc acgttttttc caaaggtctg aagaagaaaa tgctgattta aagagtgcag
     9721 agaataatga ttattcattg ttttccctct tgcctgcata tgcgctaagt gaaattaaag
     9781 atgcgttcaa aataggtttt tatttatatt tgccatttat tgttgttgat cttgttattt
     9841 caagcatttt acttgcactt ggtatgatga tgatgagtcc aatcacaata tcagtaccaa
     9901 taaaacttgt attatttgtt gcgcttgatg ggtgggggat cctgtctaag gccttgattg
     9961 aacaatatat caatgttcct gcttagatga tggaggtaat gtgtctgata tagtttatat
    10021 gggtaataag gctctttatt taatccttat cttttcctta tggccagtag gtatagctac
    10081 ggttattgga ttaactattg gtttattaca gacagtgact caacttcaag agcagacact
    10141 tccttttggt ataaagctta taggtgtctc aatatctttg ctacttcttt ctggatggta
    10201 tggtgaggtt ttattgtctt tttgtcatga aataatgttt ttaattaaga gtggggtttg
    10261 atggacattt caagctggtt cgaaagtatt catgtgtttt taatactcct gaacggcgtt
    10321 ttttttagat tggctccatt atttttcttt cttccatttt taaataacgg tataatttct
    10381 ccatctatta gaatacctgt gatttttctt gttgcatcag gattaattac ttctggtaag
    10441 gtagacatag gttcttctgt ttttgaacat gtttatttcc ttatgttcaa ggaaataatt
    10501 gttggcctcc ttctctcttt ttgcttgtct cttccctttt ggatatttca tgctgttggt
    10561 agtattattg acaaccagcg tggggcaacg cttagtagtt caattgatcc tgccaatggt
    10621 gttgatacgt ctgaattggc aaaatttttc aatctttttt ctgcagttgt atttctatac
    10681 agtggtggaa tggtctttat tttagaatcc atacaattgt cttataatat atgcccgtta
    10741 ttttctcaat gttctttccg tgtctcaaat atcttaacat ttctgacttt attggcaagt
    10801 caggctgtta ttttagccag tcctgttatg atagtattgt tactatcaga agtattactt
    10861 ggtgtattat cgagatttgc tccgcagatg aatgcttttt ccgtatcatt aactattaaa
    10921 agtttacttg caatatttat tattttcatc tgttcttcta ctatttactt ttctaaagtt
    10981 caatttttcc tcggtgaaca taaatttttc acaaatctat ttgttagata aaatattatg
    11041 gcaaataaaa cagaaaagcc gacacctaaa aaactaaagg atgccgcaaa aaaaggacag
    11101 tcatttaaat ttaaggattt aacgacagtt gttattattc tggtagggac atttactata
    11161 atatcattct tttccttaag tgatgtaatg ctcttataca gatatgtaat aattaatgac
    11221 ttcgaaatta atgagggtaa atactttttt gcagtggtta ttgtcttttt taagataatt
    11281 ggcttcccac tttttttctg tgttctttcg gctgtgttgc caacattggt tcaaacaaag
    11341 tttgttcttg cgactaaagc tatcaagatt gatttttcag tattaaaccc tgttaaaggg
    11401 ttaaaaaaaa tatttagtat aaagacaata aaagaatttt tcaaaagcat tctgcttctt
    11461 attattctag cattaacaac ctatttcttt tggattaatg accgaaaaat aattttttct
    11521 caggtgtttt ctagtgttga tggcttatat cttatttggg gggggctatt taaggatata
    11581 atattatttt tcttagcatt ttctattttt gttattattc ttgactttgt gattgagttc
    11641 attttataca tgaaagatat gatgatggat aaacaggaga taaaaagaga atatatagag
    11701 caagagggac actttgagac aaagtcgaga aggcgtgagt tgcatatcga gattctttca
    11761 gagcagacta aatctgatat acgtaattca aaattagtgg taatgaaccc gactcatatt
    11821 gcaattggta tttattttaa tccagaaata gcgcctgcac cttttatttc tctcattgaa
    11881 actaaccagt gtgccttggc tgtcagaaaa tatgcaaatg aagttggtat accaactgtg
    11941 cgtgatgtga aattagctag aaaactatat aaaacacata caaaatatag ttttgttgat
    12001 tttgaacact tggatgaagt cctacgtctt attgtttggc ttgagcaggt tgaaaacact
    12061 cattaataag taaaggagat gtgttatatg ggagttaatt tctgtaataa aataggtatt
    12121 gatcagtctg aatttgaaat agaatcttcc atcattaact ccattgctaa tgaggtattg
    12181 aacccaatat ctttccttag caacaaggat ataataaatg ttttactcag aaagatttca
    12241 tctgaatgtg acctggttag aaaagacatt tatcgttgtg ctcttgagtt ggtcgttgaa
    12301 aaaacacctg atgatttata atcattcata ataactcttt tgttgatata aatatttttg
    12361 ttgaagatat ttatgtagtc acaggatcaa ttgtagaaaa ttgttatgtt tttgttagga
    12421 actgaactaa ataatatatg ataagacagc aaaaacgact gaccataatc cttcttttat
    12481 taggagttga taagagagac tattcatcct gtaatgttaa aactctgtta tatagtattc
    12541 gcgattatgc aaagtctgta aatgatcatg agatactaac cgagagtaat cgtttattat
    12601 ctcattgcat ttcagatagt aacggagctt tctttaaaag tagtaaatac gttcccctta
    12661 agtacttacg taaaaggaga atagcacgaa agataccaaa tgattagcac tttgctagta
    12721 agcttgctgg taaactatat tgaccgttgg gtttgttttg agactgtaat ttaaattgtg
    12781 taattgcctg tttttgatat gtttactcca acaacggaga caggtaaatt atggacgaaa
    12841 agaagcttaa agcccttgcg gctgaactgg ctaaaggcct caaaactgag gccgacctca
    12901 atcagttttc ccgtatgctg acgaaatatc ccggctctta aaacgatagt ggctcaggct
    12961 ttcatttttc agcgttcccc agaagatttc catcggagcg ttgtcgtaac agt
//
LOCUS       DAJTNN010000114        12836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTNN010000114 DAJTNN010000000
VERSION     DAJTNN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.114"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(174..1097)
                     /gene="dmlR"
                     /locus_tag="OO150_002848"
     CDS             complement(174..1097)
                     /gene="dmlR"
                     /locus_tag="OO150_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR5516926.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            1200..2285
                     /gene="dmlA"
                     /locus_tag="OO150_002849"
     CDS             1200..2285
                     /gene="dmlA"
                     /locus_tag="OO150_002849"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR5516927.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMG
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            2535..4145
                     /gene="yeaV"
                     /locus_tag="OO150_002850"
     CDS             2535..4145
                     /gene="yeaV"
                     /locus_tag="OO150_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCR5516928.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVIPAGTVFKGDN"
     gene            4177..5301
                     /gene="yeaW"
                     /locus_tag="OO150_002851"
     CDS             4177..5301
                     /gene="yeaW"
                     /locus_tag="OO150_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR5516929.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTSANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            5357..6322
                     /gene="yeaX"
                     /locus_tag="OO150_002852"
     CDS             5357..6322
                     /gene="yeaX"
                     /locus_tag="OO150_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5516930.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(6376..7491)
                     /gene="rnd"
                     /locus_tag="OO150_002853"
     CDS             complement(6376..7491)
                     /gene="rnd"
                     /locus_tag="OO150_002853"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5516931.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7572..9257)
                     /gene="fadD"
                     /locus_tag="OO150_002854"
     CDS             complement(7572..9257)
                     /gene="fadD"
                     /locus_tag="OO150_002854"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5516932.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGVGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAVVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(9462..10043)
                     /gene="yeaY"
                     /locus_tag="OO150_002855"
     CDS             complement(9462..10043)
                     /gene="yeaY"
                     /locus_tag="OO150_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5516933.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGRSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10083..10778)
                     /gene="tsaB"
                     /locus_tag="OO150_002856"
     CDS             complement(10083..10778)
                     /gene="tsaB"
                     /locus_tag="OO150_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5516934.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(10836..12746)
                     /locus_tag="OO150_002857"
     CDS             complement(10836..12746)
                     /locus_tag="OO150_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5516935.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
BASE COUNT         3044 a         3448 c         3205 g         3139 t
ORIGIN      
        1 ccaaccagat aggtccagta attcagaacc ccgtattcag cgaacacatt aacctcttta
       61 attatctttc gatcatgcgc gattaaaggt gaatatgcta accaatctgt agcggcttag
      121 aaaggagaaa atcaggtttt aacccgatat caacccgata attgaatcat taatcaggca
      181 tgctccagtg aaaaattcgg gtagtgctcg gcaaaatact ggcgtaaaaa ttctaccgtt
      241 atccgcactt tcgctgacgt cgccagcctt gaaacataaa cggcccagac gttcgctggc
      301 tggtaatatt ccggtagcac ttgcactaaa tgaccactgg caatgttttc gctaacatcc
      361 caccaggaac gcagagcaat cccctgccca tcgagacacc actgatggac aatttctcca
      421 tgattagacg acagtgggcc ggtgacttta atcgcatggt ggccttcttt attgcgtaat
      481 tgccagacac caaaaggatg atcgcgctct ttaatgacca ggcacggcaa tgcagaaaga
      541 tcggttaaat gtttcggcgc gccatgttgc gcaataaatt ccggcgacgc gcagagaatg
      601 cggtaattgg tcgccagttt gcgggcgatt aaattagggg cgatgtcatc gccaatgcga
      661 atatcgagat cgacaccttc attgaccaaa tcgaccagcc ggtcttccac atcaaaacgt
      721 aattccagtt gcggatacgc cttcgccagc gctgataacg ccggagccac cacctgtcgt
      781 ccaaaaccaa agctgctgat aatacgcagc atcccctgtg gcacctgacg cacgtcagaa
      841 agttcgtcca tcatctgacc gacatcctgc aaaatccgct gcgcccattc ataaatccgc
      901 tctccttctt cggtaatggt gacgcggcgg gtggtgcggt gtaacaacac cacgtttagc
      961 gtttgctcca gcaaggcgat gcgcttgctg acgaacgccg gtgaaacgcc cagttcttcc
     1021 gccacggcgg caaaaccagc ccgacgagcc accagcataa agacgcgcaa atcattcagc
     1081 agcggtaaat tattcatgat tcgtgtttta tgtttcacca gttacgggga ttaattcctt
     1141 ttccgtcaat tataggatgg tgatgttgtc aattttgatg gtcaggaagt gagaacccaa
     1201 tgatgaaaac gatgcgtatt gctgcgatcc cgggagacgg gattggtaaa gaagtccttc
     1261 ctgaagggat tcgcgtgtta caggctgccg ctgagcgctg gggcttcgac ttgagttttg
     1321 agcaaatggg gtgggcgagc tgcgagtatt acagccatca cggtaaaatg atgccggacg
     1381 actggcatga gcaacttagc cgtttcgacg ccatctattt tggtgccgtc ggctggccgg
     1441 ataccgttcc ggaccatatt tcgttgtggg gttcgctgct gaaatttcgt cgtgaattcg
     1501 accagtacat caacctgcgc ccggttcgtc tctttcctgg cgttccctgc ccgctggcgg
     1561 gaaaacagcc tggcgacatc gatttttacg tggtcaggga aaacaccgaa ggcgaatatt
     1621 cctcgctcgg cggtagagtg aatgaaggta cagagcatga agtcgtcatt caggaatcgg
     1681 tatttacacg tcgtggtgtc gatcgcattt tgcgttatgc cttcgaactt gcgcaaagcc
     1741 gcccacgtaa gacgctaact tatgccacta aatcgaacgg tttagccatc agcatgccgt
     1801 actgggatga gcgagtggaa gcaatggccg agaattaccc ggagatccgc tgggacaagc
     1861 agcatattga tattctctgc gcgcgttttg tgatgcagcc ggaacgcttc gatgtggtgg
     1921 tggcgtccaa tttgtttggc gatatccttt ccgatcttgg cccggcctgc accggcacca
     1981 ttggcattgc cccatccgcc aacctgaatc cggaacgcac tttcccgtcg ctcttcgagc
     2041 ctgtccacgg ttccgcgccg gatatctacg ggaaaaatat tgctaaccct atcgccacga
     2101 tttgggccgg ggcaatgatg ctcgattttc tcggcaatgg cgatgagcgt ttccagcaag
     2161 cgcataacgg tattctggca gcaattgaag aagtgattgc tcacgggccg aaaacacctg
     2221 atatgaaagg caatgccacc acgccacagg ttgccgacgc gatttgcaaa attattttgc
     2281 gttaaggtca aaccagttta tttgaaccgc gtcactgacg cggtttttta ttcgttcttt
     2341 gcagtaaata acctgcgtca tttcaccttt tattgtttcc gtttcgtgtt ttatggcttt
     2401 ccgcattctt aattgtttaa tttatgtaat attcaaataa tcaatttgtt aaacacaaca
     2461 tacaactcat taccctgcaa ataattacaa ctggcaaaag ccagagacgg gcttttatgc
     2521 ccaggagatg actcatgatg agcaatgtta agaaaaaaga tgtgccgctg ataagcatca
     2581 gcctggtggc cattcttttc atcgcagctg cattaagcct tttcccacaa caatcggccg
     2641 acgcggccaa cgcaatatac acttttgtta cgcgtacgtt aggttccgcc gtacaggtat
     2701 tggttttgct ggcaatggga ctggtgattt atttagccac cagtaaatac ggcaatattc
     2761 gtcttggcga aggaaaaccg gaatacagca cgctctcctg gctgtttatg tttatttgtg
     2821 ccggtttagg ttcttctacg ctttattggg gggttgctga atgggcctat tattatcaga
     2881 cacctggatt aaatatcgca ccgcgttcac aacaggcact cgaatttagc gttccctact
     2941 ctttcttcca ctggggcatc agcgcctggg caacttatac gctggcctca ttaatcatgg
     3001 cttatcactt tcatgtgcgg aaaaacaaag gtctgagcct ttccggcatt attgctgcta
     3061 ttaccggcgt tcgcccgcaa ggcccatggg gaaaactggt tgatttgatg ttcctgatcg
     3121 ccactgtcgg cgcactgact atttcccttg ttgtcaccgc agcaaccttt actcgtggac
     3181 tttccgcact gacaggttta cccgacaact tcaccgtgca ggcatttgtg atcctgcttt
     3241 ctggcggcat tttttgccta agctcgtgga ttggtatcaa caacggtttg caacgtctga
     3301 gcaaaatggt tggctggggc gcgttcctgc tgccattact ggtgctgatt gtcggcccaa
     3361 ccgaatttat taccaacagc atcatcaatg ccatcggcct gaccacgcaa aacttcctgc
     3421 aaatgagctt attcaccgat ccgcttggcg atggttcatt tacccgcaac tggaccgttt
     3481 tctactggct gtggtggatc tcatacaccc ctggcgtagc aatgtttgtc acccgcgttt
     3541 cccgcggtcg taagattaaa gaagttatct ggggactgat cctcggcagc accgtcggtt
     3601 gctggttctt cttcggcgta atggaaagct atgccattca tcagtttatc aatggcgtaa
     3661 tcaacgtccc acaggtgctg gaaacactgg gcggcgaaac agctgtacag caagttctga
     3721 tgtcgttgcc agccggtaaa ttgttcctcg ccgcatacct gggcgtgatg attattttcc
     3781 ttgcctcgca tatggatgca gtggcctaca ccatggcggc gaccagtacg cgtaatctcc
     3841 aggaaggtga cgatcctgac cgtgggctgc gtcttttctg gtgcgtggtg atcactctga
     3901 tcccgctttc catcttgttt accggtgctt cgctggaaac gatgaaaacc accgtcgtgc
     3961 tcacagccct tcccttcctc gtcattttac tggtgaaagt cggcggattt attcgctggc
     4021 tgaaacagga ttacgccgac attccggctc atcaagttga acattatctc ccgcagacac
     4081 cggttgaagc cctggaaaaa acaccagtga tccctgcggg aaccgtattc aaaggcgaca
     4141 actgagcgcc atcatcctaa cgataaaggt atccctatga gcaatctgag ccctgacttt
     4201 gtactacccg aaaatttttg cgctaacccg caagaggcgt ggaccattcc tgcccgtttt
     4261 tataccgatc agaacgcgtt tgaacacgaa aaagagaacg tcttcgccaa aagctggatt
     4321 tgcgtcgctc acagcagcga actggcgaat gccaatgatt atgtgacgcg tgagatcatt
     4381 ggcgaaagca tcgtgctggt acgcggtcgt gataaggttt tgcgcgcgtt ctataacgtg
     4441 tgtccgcacc gtggtcatca gttgttgagc ggtgaaggaa aagcaaaaaa tgtgattacc
     4501 tgcccgtatc acgcatgggc attcaaactc gatggcaatc tggcccatgc acgtaactgc
     4561 gaaaacgtcg ccaatttcga tagcgacaaa gcgcaactgg ttccggtgcg tctggaagaa
     4621 tatgccggat tcgtcttcat caacatggac cccaacgcca ccagcgtaga agatcagtta
     4681 cccggtctgg gcgcgaaagt gctggaagcc tgcccggaag tccacgatct gaaactggcg
     4741 gcccgcttta ccacccgcac gtctgccaac tggaagaaca ttgtcgataa ctatctcgag
     4801 tgctatcact gtggtccggc gcatccaggt ttctccgact ccgtacaggt tgatcgttac
     4861 tggcacacca tgcacggtaa ctggacgctg caatacggtt tcgccaaacc gtccgaacag
     4921 tcgtttaaat ttgaagaggg tacggatgcg gcattccacg gtttctggct gtggccgtgc
     4981 acgatgctga acgtcacccc gatcaaaggg atgatgacgg tcatttatga attcccggtg
     5041 gattctgaaa ctaccctgca aaactacgat atttacttca ccaatgaaga gttaaccgac
     5101 gagcaaaaat cgctgattga gtggtatcgc gatgtgttcc gtccggaaga tttacgtctg
     5161 gttgaaagcg tacagaaagg gctgaaatcg cgtggctatc gtggtcaggg gcgcatcatg
     5221 gccgacagta gcggtagtgg catttccgaa catggtatcg cccatttcca taatctgctg
     5281 gcgcaggtgt ttaaggacta atgacatcgg cggcggtatt ttccgctgct gggctgattt
     5341 ttgatggagt acagcaatat cagactatca aatgtttgaa gtacaggtga gccaggttga
     5401 accccttacc gaacaggtga aacgcttcac gctggtggca accgatggca aaccgctgcc
     5461 tgcgttcacc ggaggaagtc acgtcattgt gcagatgagc gatggtgata accagtacag
     5521 caatgcgtat tcactactga gttcgccgca tgacacctct tgttatcaga ttgccgttcg
     5581 gctggaggaa aactcgcgcg gcggttcccg ctttttgcat cagcaggtaa aagtgggcaa
     5641 tcggttaacg atttcaacgc ctaataacct gtttgcgcta attccctcag ccagaaagca
     5701 tctgtttatc gcgggcggta ttggtatcac ccctttcctg tcgcacatgg cagagctgca
     5761 acacagcgac gtcgactggc agctacatta ctgctcgcga aatccagaaa gttgcgcatt
     5821 tcgtgatgag ctagtccagc atccgcaggc tgagaaagtt cacttgcatc actcatcaac
     5881 cggaacacga ctggaattag cgcgattatt ggcggatatc gaacctggca cacacgttta
     5941 tacctgtggt cccgaggcgc taaatgaagc ggtaagaagt gaagctgcgc gtctggacat
     6001 cgccgccgat acgctgcact ttgagcaatt tgctatcgaa gacaaaaccg gcgatgcctt
     6061 taccctggtg cttgcccgtt ccggaaaaga gtttgtggtg ccggaagaga tgactatttt
     6121 gcaggttatt gaaaataata aagccgcgaa agtggaatgt ttatgtcgtg aaggggtatg
     6181 cggaacctgc gaaacagcaa tactggaagg tgaagctgac catcgggatc agtattttag
     6241 cgatgaagag cgtgccagcc agcaaagtat gttgatctgt tgttcgcgtg cgaagggtaa
     6301 acgcctggtg ttggatttgt agtttgccaa agccggatgt ggcgctgagc gcgcccagtc
     6361 cggcttcgga agattttact gcggatattc ctgcaataaa ttgtgtaatg cttccgccat
     6421 cagctcacca cgccagccgg aaatcagctc cggcaaattg ttctgcggtt tcagtttcca
     6481 gtgccagttc agcagttggt tgatttgccg acgcgatgcc agcaattcgg cgctgatctt
     6541 atgcgtttcg ctcacgtcag taatcagcga cttaatcgct ttaaacgctt tacgataacc
     6601 aggcatgtcc atcaggttaa gcatcggctg cggtaaggcc tcttccggca atgtctgcgc
     6661 tttttccacc agcgtcagca gcgttttacc gtgaaagcgg atttcgctac cggataaacc
     6721 caggctgtcc agttcgccta aacttcccgg catataacgc gcgaccgacc acaaatgctc
     6781 ttcacgcacg acaaagttca ccgccagatc acgctctcgc gccttgcgca gtcgccagtc
     6841 ggctaacagt tgcagacagg ccagttggcg tgtgcgtaac tgccaggcat tggtgatatc
     6901 acgccaggca tcttccggcg caacgacttc ctgacgacgc atttgcatca ggcggcattc
     6961 atccagcgcc gcaggtagcc agccggaggc ctccgtttct accataagct tggcggtgat
     7021 cggtaacaga taccagacat ccgccgctgc gtattcacac tgacgttcgg tcagcggtct
     7081 ggccagccag tcggtgcgcg attcactctt gtccagcgta acgccggaat actcttccac
     7141 catggaagcg aaaccccatg acatcgggcg tccgcagaag gcagaaagga tttgcgtgtc
     7201 aatcaagggt tgtggtaatt cgccaaagac attgaggaac acttccagat cttcactgcc
     7261 tgcatggaga aattttgtga tggacggatc gcgcaggatc gctttcagcg gtgaccagtc
     7321 ggtgatcccg agaggatcaa ttagcgccag atgctcgcca tcgaaaagtt gaatcaaccc
     7381 cagctgcggg taataagtgc gcgtacgaac aaattcagta tccagggcta tcgccggaaa
     7441 ggcacggacg gcttcacaca aagaagccag cgcatcgtcc gtggtaatca tttggtaatt
     7501 caaattgttt tctctttagt gggcgtcaaa aaaacgccgg attaaccggc gtctgacgac
     7561 tgacttaacg ctcaggcttt attgtccact ttgccgcgcg cttcgtcacg taattctcgt
     7621 cgcaaaattt ttccgacgtt agatttcggt aactcatcac gaaactccac cagcttcggt
     7681 actttgtatc ccgtgagctg acggcggcaa aaagtcacca gtgactcttc ggtaagcgat
     7741 ggatcttttt tcactacgaa gattttcacc gcttcaccac tggagccgga aggtacgcca
     7801 acagccgcga cttcctgtac gccaggatgc tgcatgacga catcttcaat ctcgttggga
     7861 tagacgttaa aaccggaaac cagaatcatg tcttttttac gatcgacaat gcgcaggaat
     7921 ccttcttcat ccattaccgc gatgtcgccg gtgtgtaacc agccattttt gatgatttca
     7981 tcggtagcat cgggacgctg ccagtaaccc agcatcacct gcggtccttt gacacaaagc
     8041 tcacccggtt gacctggtgg tacttcatta tcatcatcat ccaccagttt ggcttccgtc
     8101 gacggcaccg gcaaaccgat gctaccacta tgataatcaa tatcatatgg gttaacgctg
     8161 accagcggcg cacactcggt aaggccatag ccttccagca gatactgtcc ggtcagtttc
     8221 acccaacgct ctgccaccac ttgctgcact ggcatcccac cgcctgcgga aagatgcaga
     8281 ctggagaaat ccagctgctg gaactcttta ttgttcagca acgcattgaa caaggtgtta
     8341 acgccagtga tagcggtaaa cggatatttc gctaactctt ttaccaaccc tggaatatca
     8401 cgcgggttag tgataagcag gttctgccca cccagttcga taaacagcag gcagttaatg
     8461 gtcagggcaa aaatgtgata cagcggcagt gccgttacta ccagttcttt acctggatgc
     8521 agcaacggcc cataggtggc gttgacctgt tccaggttcg ccagcatatt gcggtgagtc
     8581 agcatcgcgc ctttcgccac acctgtggtg ccgccggtgt attgcaggaa ggctaaatct
     8641 tccggcacca gttcgggttt gacgtactgc atccggtagc cgttatgcag tgcgctacga
     8701 aatgaaatgg catctggcag atggtatttc ggcaccaaac gcttgatgta tttaacaacg
     8761 aaattgacta ccgtgccttt tgccgtagat agctgatcgc ccatacgggt cagaattacg
     8821 tgctgaacgg cggttttatc aaccactttt tccagtgtgt gagcaaagtt agacacgata
     8881 acaaccgccg atgcgccgct atcgttaagc tgatgctcaa gctcacgcgg ggtatacaac
     8941 gggttaacgt ttacgacgat catcccggca cgcaaaatgc caaacagcgc caccggatat
     9001 tgcaataaat taggcatcat caacgcaacg cgatcgcctt tcttcagccc caccccttgt
     9061 tgcaaataag cggcaaacgc gcgactgcgt tcttccagct tgcggaaggt catcacctcc
     9121 cccatattca caaacgcagg ctgatcggcg tagcgcgcga ccgactgctc aaacatatct
     9181 accagagatt gataacggtc agggttgatc tccgtcggaa cgtccgcggg ataacggtta
     9241 agccaaacct tcttcaattc ttcacctcta aaatgcgtgt tcgtcgtcat cgcaacccca
     9301 aatgatatac atgccgttaa cataatatta actcatcata ccagcttgat aattacccaa
     9361 cgaaaaggtt gcgaagcgcg tcactattta tttttatctt taccgtaaga atgcagaaac
     9421 agcggaccag ccgctgtttc tttttcttta aaaacaagca attattcagt tacaactgtt
     9481 tgtactctcg cgggaccggg attataccag ccccatccgc cgtagccata gggccagcca
     9541 cgaccgccat aaaaccatgg atcaatcggc tgaggcggca taatcacctg ctgggttaaa
     9601 tgccaacgtt tgtaacccgt tacttgcatc accataaatt tatagggcgt attgccgatt
     9661 ttgccgtcaa ccgcaccagt gattggcccg actaccgtaa ccagttgtcc acggaaatcc
     9721 accgggtcca gaaaaccgtt cacatcggca taaatgcgac cgcgagaagg ttctcccagc
     9781 gtcggtctgg ctccgctgtc cagcggtacg gtagcaattt ccaggcgggt tttcccttgc
     9841 tggttttgta ccgcaaccac tttgccacca aagcgtgcct cctgaccaac gtacagctgc
     9901 ggcgcactca tcacccgaac taaatcttgt tgcggcgtgg gactgcggcc tttaatggcg
     9961 tccggcacag tgacacaacc gctcagcatt agcgcaaacg tacctgccag tatgcctttg
    10021 ataacattct tttgaaccgc catgatgcga ctcctttttt tctcagagca tactcttaag
    10081 attcattctt tgcccggaag tttcttccat gcgacgttgt tacgtaaata aaccggttcg
    10141 gcatgttcca ccgccaccgt tttaccctcg gcaaatatct gacacgcaat cggcagcata
    10201 tcttccgtcg ctggcagtaa aacttctccg tcgcgcaaaa ccagcccgct ctctttaccg
    10261 agatccggcc aggcttgcca gcccgtccct accgttaccc attcgccgga aagctgttgc
    10321 attcgttcat gaacgagttc gggtttgagt acggcttcgg tttcttcacc gcgccagata
    10381 ccgttttcat cacgctgata ttcggcccag taaacttcgc ccatccgcgc atcaatggca
    10441 gccagcacgc gggttgcgcc gtttttgcgc cacgcccctt gcgccattgt cattagcgtg
    10501 gagacgccaa tcatcggcag ctctgcgcca agtgccagcc cttgcgcaat gccaatacca
    10561 atgcgcacgc cggtaaagct accggggccg cgcccgtaag ccagagcgtt aatatcagtc
    10621 agggaagttc cgctggtggt caggatatcc tgcaccatcg gtaagattcg ttgagtatgt
    10681 tcacgagggc aaagctcaaa atgagcgttg acagtaccgt cgttccacag ggcgacagag
    10741 caggcctctg tcgcggtatc gatagccaga attcgcatgg gtcttcgtgc ttagatcaat
    10801 aaaaaggcgc gcatcatacc atactccgta acaaattacc tggaggatgg tatcgcaagg
    10861 aaacgaaccg cacgggcaat gtcacgggtg cgcggcgcgg gcggcagact ggcgagaaac
    10921 gtcgcgccgt aagggcgcat caccagccga ttgtcacaaa tcaccaaaac cccgcgatcg
    10981 tcggcgtcgc gaatcagtcg ccctaccccc tgcttgagag taatcaccgc atccggcagt
    11041 tgtacttcat cgaacgggtc gccaccgcgc aaacggcaat cttccatgcg cgcttttaac
    11101 agcgggtcat ccggtgaggt aaacggcaac ttgtcgataa tcaccaatga caatgtatcg
    11161 ccacgcacgt ccaccccttc ccagaaacta ctggttgcca caagaagcgc gttaccggcg
    11221 ctgacaaatt gctgcaacag ttgccctttg ctggtttccc cctgcaacaa aacgggaagc
    11281 gtcatggtag cgcggaactg ctcggccaga tcacgcatca tggcgtgcga ggtacaaagc
    11341 ataaaacaac gaccgttgtt agcttcgatg atcggtcgca gcattgccgc taactggcga
    11401 gcagaacctg gctggttggt ttgcggcaga ttgcgcggca cacagagtaa cgcctggcgg
    11461 ctgtaatcaa acgggcttgg caatagcaac gactccgcct gttctatccc aagccgcgag
    11521 gtgaaatgat gcagatcgtc gttcaccgac agcgttgctg aggtgaagat ccagctacca
    11581 ggcttttgcg ccattaactc tttgaattta tccgccacgc tgagcggcgt gagagccaga
    11641 gtaaaatggc gcgaagtgca ttcgtaccag tagctgtagc ccggctgatt gatctctttt
    11701 agccgcttca gccgtgtgcg atacaacgta gcgcgctcaa atgccgcatc cagcaaggcg
    11761 gaacgcccca gtgacagttt cgccacgtca taacaaagtt ccagggtgtc atcgagcagt
    11821 aaaaatgccc gctgaatttg cggattagct aacagctcac gcaggttacc gcgataacct
    11881 ggctcaccga gttgcagacg aaaatcctgc gcactctggg caagacgatc agcgcacttt
    11941 tgtaactgct gggtgtcttt taattcggtg cggtaggcga tggtgatgtc ttttgccagg
    12001 tcgagcagtt gtcgactgga gagtgactga ccaaaatact ggctggcaat gtccggtagc
    12061 tggtgggctt cgtcgaagat catgacgtcc gcttccggga tcagttcgcc aaatccactc
    12121 tctttaacca ccatatccgc cagaaagaga tgatggttta ccaccaccac atcggcgtcc
    12181 atcgcttttt tacgtgcttt gaccacaaag caatctttat acatcgggca gtcgctgcca
    12241 agacagttgt cgttggtgct ggtaaccagc ggccacgcct gtgaatcttc cgccacgctg
    12301 acgcaggtgc tgatatcacc atcgactgtt tgattagacc aggagcgcag caggatcaca
    12361 tcgcttaaga tttgtaccgg cagatcgccc cccgccagcg cctgctgttc gagacgttcg
    12421 aggcagaggt agtttgagcg ccctttcagc agcgccacgt tgcccgtata tttcaatgcc
    12481 tttgagactg ttggcaaatc gcggctgtag agctgatcct gcaacgcttt tgagccggtc
    12541 gagataatga ctttcttttt cgcccgcagc gcaggagcca ggtaagcgta ggttttgccc
    12601 gtaccggttc ctgcttccac caccagcggc tggccttttt ctatcgcctg ggtgacggct
    12661 accgccatct gtcgctgtgg ttctcgcggc ttaaagcctg gtatcgcttt cgccagctga
    12721 ccgtctggtg caaaatcgtc cgtcacacta ccccctgttg atttgaacgg ggattatgtc
    12781 aggatgaggg cgctttcgcc agttgaagtg gtgacggcaa cctcacattg tggcag
//
LOCUS       DAJTNN010000115        12778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTNN010000115 DAJTNN010000000
VERSION     DAJTNN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.115"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(23..1009)
                     /locus_tag="OO150_002858"
     CDS             complement(23..1009)
                     /locus_tag="OO150_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5516936.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVRADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(1006..1902)
                     /locus_tag="OO150_002859"
     CDS             complement(1006..1902)
                     /locus_tag="OO150_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5516937.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPRAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(1899..2921)
                     /locus_tag="OO150_002860"
     CDS             complement(1899..2921)
                     /locus_tag="OO150_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5516938.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(2923..4473)
                     /locus_tag="OO150_002861"
     CDS             complement(2923..4473)
                     /locus_tag="OO150_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5516939.1"
                     /translation="MKRSIPFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(4487..5068)
                     /gene="ddpX"
                     /locus_tag="OO150_002862"
     CDS             complement(4487..5068)
                     /gene="ddpX"
                     /locus_tag="OO150_002862"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR5516940.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(5326..7725)
                     /gene="dosP"
                     /locus_tag="OO150_002863"
                     /gene_synonym="pdeO"
     CDS             complement(5326..7725)
                     /gene="dosP"
                     /locus_tag="OO150_002863"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR5516941.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNHDYL
                     LSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(7750..9131)
                     /gene="dosC"
                     /locus_tag="OO150_002864"
                     /pseudo
     CDS             complement(7750..9131)
                     /gene="dosC"
                     /locus_tag="OO150_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(9503..10822)
                     /locus_tag="OO150_002865"
     CDS             complement(9503..10822)
                     /locus_tag="OO150_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5516942.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(10953..12488)
                     /gene="gadC"
                     /locus_tag="OO150_002866"
     CDS             complement(10953..12488)
                     /gene="gadC"
                     /locus_tag="OO150_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5516943.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         3233 a         3241 c         3152 g         3152 t
ORIGIN      
        1 gccgcgtaac gttaataacg tgtcagacac ttatgacctc ctgctgcgga taccaacagg
       61 cgcagcgctg gttgttgtca ccacacgctg tcagcgccgg gacgttttca cactgtgcac
      121 ctgccgcata gcaacgatcg cgaaaagcgc agccgtcagg caaatgggtg aggtttggta
      181 ccgtccctgg aatggcgggt aatagctggc gtggtactcc atgttctggt gcacattgga
      241 gcaaaccaat ggtatacgga tgccggggat gatggataac gtctgccctc acgccgcttt
      301 caattacgct tccggcatac atcacgtaaa cgctatcgca cagttgcgac accacggcca
      361 tatcatggct gatgaacaga accgcagttc cactggcgcg ggctttatgt ttaagcaaac
      421 gcagtacctg caactgcacc gtgacgtcca gcgccgtagt cggttcgtcg gcaataatca
      481 attgcggctc gcaggagaat gccagcgcaa tcattacccg ctggcgcata ccacctgaaa
      541 gctcaaacgg atagcgcgac ataacttcca cggcatccgg gatttgcatc tcttccagca
      601 ggacaatcgc tttagctctg gcttcccgac gacttattgg ttgatgatgg cggatcacgt
      661 ccatcatctg aagacctatt cgacgtgtcg gattgagggc ggtcatcggt tcctgaaaga
      721 tcatcgccac tcgtgcgccg cgccactgac gaagctgttt ttcccgagcg ttaagaacat
      781 cgtctcccag cagtgaaatc tgcccccgat gtacgcaata actgcccgtc ggtagcagac
      841 gcataatcag cattgcggtg actgatttac ctgagccgga ttctcccacc agaccgacaa
      901 tttcaccgcg gttaatctgc aaggacacat tgttgagcgc gtgaacatca ccgttaaaac
      961 cggggaaact caaatgcagc tgttgaatgt ccagaacggg ctgggtcatg actgctttcc
     1021 tcctgctttc gggtcaagca gatcgcgaat accatcacca aagagattaa acccaacggc
     1081 ggtaagcaaa atcgctgccc ttggaaatgc gcaataccac cactgatcga gtacatagtt
     1141 gcgaccattc gctaccatcg cgccccattc agcactcggt tgttgagcac ccaggccaat
     1201 aaatcccaac gtggcggcca ttaaaatcgc gctaccgata tccagcgatg cctgcacgat
     1261 cagcggcggc agggagttac gtaaaatatg ccagctgatc agatgccaac gcgacgcacc
     1321 aaaggttttc gccgcctgaa cataggtata ctggcgtaca actaatgctt gcccccgcgc
     1381 caggcgcaca taaaagggaa ttcgcacaat agcaatcgcc agcatggcgt taaataaact
     1441 cggcccgaga gcggcagcca gtgccattgt cagtaccaac gaaggaatcg acagcataat
     1501 gtccatgatg cgcatgatga tggcgtctgc gcgtccgcca agcacaccgg atagacatcc
     1561 gagtagcgaa ccaatcatac ccgcaatggc aaccacgact aatccggcga gaattgattg
     1621 ctgactgccg accagtacgc ggctgaacag atcgcgtccc acttcatcgg tgccaaacca
     1681 gtgcgccgca gaaggcggca aaaggcgggc ggttaaatca atggcgttgg gatcatgcgt
     1741 cgcaagccac ggtgaaaaaa acatcatcaa tagcattaat acaatgatga ctgcgccggt
     1801 cacggtgagc ggactgcctt tcagcatcca taccagtttt gcaccgttaa atcgcgtttg
     1861 tttttgtggg cgtacggcgg acgtttcctc gcttagcatc attcaccacc tccacgtccg
     1921 atacgcggat caatccacaa atagagcaaa tccaccacca ggttgaccag cacataagca
     1981 aatgaaacca cgacggcaaa gcccatcact gccgggaagt cgagcgcctg tattgatgtt
     2041 actacccatg cacccattcc gggccaggca aaaacggttt cggtgagcac tgcgccatac
     2101 aacaaatcgc ccagcgccag acccaatacg gtaatcgatg ggatcaacgc attgggtagc
     2161 gcataacata aaacgatata ccagccgggc aagccgctgg cccgggcggt acgaatgtag
     2221 tcttcactca attgttccag catcgctgag cggatctggc gagcgacaat tcccaggtga
     2281 acgaacgcca gcgttaatgc cggtaagatg agatgttgca acgcattgaa gaagacttca
     2341 ccgttgcctt caagcagcgc atcgagcaga taaaagccgg taacgtgcgt tggtggatcc
     2401 agccagtcat caagccttcc gccgccggga agaatttgca gatgaccata aaacagcaca
     2461 atgacgccca gtccaagcca aaacgcaggt gtggagattc cggtaatggc cattattcgc
     2521 accagatgat ccagccagcg atttcgccag actgcagaga gtatgcccag cgggatgcca
     2581 atcaggagtg ccagcagcag ggcacaaaaa gccagttcca gcgtcgccgg gaaaaatata
     2641 cgcaactctt ccaacaccgg acgcccggta cgaatggatg ttcccaggtc accatgaaac
     2701 aggtcgctga cgtagcggta aaactgtaca tacagtggct ggtccagtcc cagttgctgg
     2761 cgaatatttt ccacgatagc atcgctggcg cggtcacccg ccagtaaccg cgccggatcg
     2821 ccagggatca gatgcgagat aataaacgta atcacgcaga cgcccgccat cacgagcacc
     2881 agcccccagc agcgttggcg taaaatactc cagaaggtca cgttatttac tcatggtatt
     2941 gatattgaag acctgttcca gcatgggatt gaacacaaag cctttcacct ctttgttcat
     3001 cgccagttgg tagtttttct ggaacaggta cacataagca gcgtcatcaa tgacgatttt
     3061 ctgtgcctgc tggtagtccc gggtacgctg cgtctggtcg gtggtcgcaa gcgcattgcg
     3121 cagtaactta tcgacctcac tgttttcata gaacgagcgg ttacccggca gacctttttt
     3181 gtctgactca aaccagtaat tcataaacat atacgggtcg gcaaaatccg gactccagtt
     3241 gccaatcgca atgtcgtaat cacctttacc cactctgtcg cgcatggtgg cgttcgccag
     3301 cttttccagc ttcacattga tgcccagctt gttgagactg gattgtgtcg ccagagcaat
     3361 aggctcccag ttcggatcct tatcggagta gagaaacgtc aggctggtgg gtttgctcgt
     3421 cactttatcc cattcggctt tggctttcgt ttcgtcatgg ttgtattgca ttgccgtcgc
     3481 atcgtagccc cacatgcctt ccggaatcgg gccgcgcatc tgttttccgt taccactcag
     3541 aatgccgtta accatgccct gataatcggt agaccaggaa atggcccgac gcagatccgc
     3601 ctgattaaga ggcgctttgc tgttattcag atacaggtag gtgacgcgca gtgacggata
     3661 ctctgccaca ttgactttgt tttcctgctt cagggcgttg agttgatcca ccggcagcgc
     3721 atcggcaatg tcaatatcac cacgggagag ctgcaggcga cgggaggcac tttcaccaat
     3781 aatttttacc gatactcgct taaagttcgg tttattgcca gggtaatgcg gatttggcac
     3841 cagaactaat tgctgacctt tttgccagct tttcagcata aatggtccgg aaccggcggt
     3901 attttgcgcg aggaagccgc gggcatcatc cgctgcatgt tcctttaaca ccgccggatt
     3961 gataatggat gcaccgtcat tcgccagcgt gtagaggaac ggtgcgaatg gctggctaag
     4021 ggtaaacttc accgtatgtt cgtcgggagc atcaatcttt aaatctttgg gaaatgcttc
     4081 tgctggcccc tggccgattt ttagtagccg ctcaaaagag agttttactg cttcggcagt
     4141 gaccggtgtg ccatcggcaa atttagcatt gtccttcagg gtgaacgtcc actctttttg
     4201 atcgtcagac gctttccagc tacttgccag atcgccttca acgtcggttg agcctttatc
     4261 accgtccgtt ttgtactgaa ccagccgctg ataagacggg taggtcaccg tccagtcgtt
     4321 attatctatt gttaccgccg gggcgagggt ttgtggatcg gcggccttac caatcaccag
     4381 catatctttc ggtacggcgg cgtgcgcaac cgggaaagtt gtggcaagga cgagcgcgag
     4441 caatgtagga cgaaacggta tcgatctctt catgacagtt tcctgattaa ctgacgtgct
     4501 gtgtgccagg ggatataaaa caggtgaatt gatcagcgag cagtgggtaa ctcgctgcct
     4561 ggggtaattc gaagtgccac cattcgctgg agataccgac aaaaccgcca ccagtcataa
     4621 tcgcattcag caacagccga ttgcgctgag cggcgggcgg gacggaaggg tgataggcat
     4681 gggagcgctc gtgcatttca tcgaaccctg cgcccatatc gaggatgttc ccgtgctcat
     4741 cacgaagcgt caggtcgatc gccgtgccac ggctgtgatt agaaccgacc gtcacatcaa
     4801 caacatattg cgggtctggg caggcttgcc acaacatcgc ctgtgcttgt tgtgggcgat
     4861 acgcatcgta aatcaccagt tgtaagcctg acagctgggc gatactgata cttttcgcca
     4921 gcgcggtaat cgcatccttg tgtagcagac aacgcgcttg ctgataaata gctttacctg
     4981 tgatgttatc agcgcaggcg tatttcaatt cgatctccag atcagggaag attacggcta
     5041 aatcaaccag ttcggtggta tccgacataa acggttcctg tataagacaa aaattgctgc
     5101 gctttccgct tatgcagatc tcatgccatg ccgggataag cgccagaatg ctggcttaaa
     5161 gttatatttt ttaacttttg atcaacattt gtgcagcgta gtgcagtttt ggtgcaagag
     5221 gggaagttaa ggaaggaatc tcccggaatc gtagatgaaa tcacagtatt taagtgacgg
     5281 tgtcacgtta aatgaaaacc cgcgagtgcg ggcgagagga atttgtcaga ttttcagcgg
     5341 taacacgctg ctcatccagc ctggaatttc ttcggcgggt agggggcggg aaaagaaata
     5401 tccctgaata acgcgacagt ggatcttgcg tagcatctca aattgctctt tggtttcgac
     5461 gccttccgcc acgacggtta aattgaggct ttgcccaatg ctggtaatgg cttcaagtaa
     5521 ggcaaggatg cgtttttcgg tcagacaacg atcgacaaaa cttttgtcaa ttttgatttc
     5581 cgttaccgga agactgacta agcgggataa tccggaaaag cccgtaccaa aatcatctac
     5641 cgataagccc acgcccatat cacgcaggat ctgaatgcgc ttaaagattt cggtatcgtg
     5701 ttccatcatc atgctttccg tgatttctac cgtcagctgg tggccgtcaa taccccaggc
     5761 gtgcattgca tcagacacct gattaggcag ttgattactg cgaaagtgca gtgccgacaa
     5821 gttaacggaa agcgccggga tatgaatatt ctggctacgc cattctgcta actgacggca
     5881 agcttccgcg atgacccagc gcccaatatt ttcgatttca ccaatctctt ctgcgagagg
     5941 aataaaccgt gaagggggca catgaccatg ctggggatcg tgccagcgcg caagggcttc
     6001 gatgccgtac agttcacccg tttctgcgaa gatttgcggc tggtaaacaa gtttcagttg
     6061 gttattgcta atcgcttctt tcagcgctgc gcctaaaacc aaacgctctt ttaccatttc
     6121 gttcatcgcc gggctgaaga actgccagcc gttaccgcca ttcttgcgaa tataatctat
     6181 tgcattgtga gcagtggaga gtaggtaatc gtggttttta cccacgtcgt agctgatgcc
     6241 aatactcaag gtaagcggga atggtttatc gtcaatcatt atcggcttgc tgaccacatt
     6301 ccgtagctca tcggcgatct gggtaatgtt actgacgtcg ttttccaggc tcacgaggac
     6361 aaactgcgta ccttcgatac gacagagata ctgatccggt ttgagttttc cacgaaagcg
     6421 attgaccact tccagcaatg cctgatcggc ccacgcatag ccaaggctat caatcacatc
     6481 ctgaatatgg tcaacaccga tgagatacac caccggagag acggctttgt cgaccaggtc
     6541 atcgaggtaa ttgtgcaggt tattgcgatt tggcagaccg gtcatcggat caaattggat
     6601 gagttgttca atatgctggc ggcttttttc ctgttccagc gccagcgcgg ccatatgctg
     6661 actgatatct gccacgcgtt cgataaaggc gctggtttct gctcctgacg aggttttaat
     6721 ttgcaggatc cccgcaggcg cgccatcacg ctgacgaatg gtcgctgacc agctttgcgc
     6781 attttgaact tctgcaccgt gcgaagatga cgcccagtgt atcggcatcc cgttgcgcag
     6841 tgcgaacagc gaaacatgcg attcgttgag aacagattcg atgttacgac aaatgatttc
     6901 ccccatttca tgaaatggcg ggctgctgca catggcggcg agaatattgc cttcaagctg
     6961 gcggatttgc cgttcttcgg tgatatccga gaaagtcatt accaggttct gcagatgcgc
     7021 gagcacgtca taaaccgggc tgatagaggc tttaatccag attttttcac cggtgcgcgt
     7081 caacagcaga aattcgtcct gatcgcgggc ggttttccat agcaactgtt gtaaacgaat
     7141 gcggttatcg gcagggaatt caggaatgtt caggagtgta tcgggctgca taccgctggc
     7201 ttcgctaatg cagtaaccaa acatttcggt aaatgcgcga ttgcactgca caatatggcg
     7261 ttccggatcg aggacaatca ccggtcggtc gagatggtca acggcaataa tcaattgtcg
     7321 ggtctgttct ttttgcgcca tttctacgct ggcatcccgt accagcgcca ggtaataaac
     7381 tttcccctcg gcgctcactt tcgatagcgc aaaacgggtc cagattttac tgccgtcttt
     7441 tttctccagc tgcagctccc gactcatccc ctcgacgcgc gctttaccac cttcacggtt
     7501 atgacgaatg tattcaggat gcgcaggacg caaatcccgc ggaatcagca tatcaatgtt
     7561 attgccaatg acttcttcac gtttgtaccc ccagagcttc tctgcggcgg ggttgaaaaa
     7621 catcacttca tcattttcgt taattaacac cgcgcccatc atattttgtt caagggcggg
     7681 gaaaaaaatg ccatcggcgg cattttccgc atcggttagc ttcatgatta cctctgcatc
     7741 ctggcgcatc taaagactgg ctttccagag ttcaacacgg tttctacctc gtcttttggc
     7801 gatatacaga gcttcatcgg ctatttgaat gaggcgctca tagtcaggat gaccattaaa
     7861 catggcggca ccgatggaaa gtgagagggc aatatcctcg ccgtttgcgg ctttcagttt
     7921 ggttttctcc acccgactgc gaatacgttc tgcggtacgt aacgtttcgt tttcagaagc
     7981 ttcagtcaaa acaatgataa attcatcgcc cccgtagcga aaaacataat cactactgcg
     8041 gacgttgtca taaaaggcct gagagacttt acgcagaatt tcatcgccag tgttatggcc
     8101 ccacgtatcg ttgatctctt tgaatttatc aacgtcaata atcagcactg acagcggtgt
     8161 accggtccgg ttggcatggg caatttcgcg tttgaagata gtcggcagga agcggcggtt
     8221 aagtaatttc gtcagtacat ccataccgac ttcgtggcgc gatacttctt caaacaattc
     8281 acgcagcaag gtaataattt gcgatacagt atttcttatc tgtaataaaa atttcacccg
     8341 cagacttctg ttattcaaat tcctggtgtt acgcatggtt tgattgaata taccgtcgaa
     8401 atcctgaatc agacgggaga tatgcctact tcggcaatgc cactaaaata atgtcgacct
     8461 ttatggttaa accacaggcc aaaatcagcc tggcttaaag gcaaactact gcctaaatca
     8521 gaatccagca ggattttata gataatatct atttcccatg aaagtattga ggctatttgc
     8581 cgttcttttt cttcttcggc gttttctagt aacgagaaga tacgatagtt ttcatcttcc
     8641 tttgaggcac tactgtcact aaaggtaaac gcgcgggtca tcacttccat agcgatatca
     8701 atactgttaa tcgagaaatg gtagacctga agtttttctg cggcagaata atccgaagag
     8761 aagattaccg gatagaggat ttttttcagc acccgaaacc ccatctcgac aatttctacc
     8821 ggaattccta tgcgggcatg cacttccgcg acggtatgct ggatttgtat tagcctttcg
     8881 acatcgtcaa cctgggcaga aagcacgtta ataatccagc gttccatcgc acttttcaac
     8941 tgccgctcaa cttgttcatt actcaagaat tcttcggcat gcgggtcgat gcggacaatt
     9001 cgataaaact cgatactcag ataatgagca tgcgcaacgg caatttccgc ggctttagca
     9061 cgaatgagtg gatctgcccg ttcgacaagt ccggtccact catctttcat tcttttaaaa
     9121 tacatctcca taattcacac ccttataagg ctgggaaatc agacggaatc aaaacgcaac
     9181 gtgcgagatc gactaactgc accatattct cctgaaatat gaagatatac tgaaaagaaa
     9241 taagcgactt aggacagttt caatctacgc tactgttctt cagaagagta tagcccatcg
     9301 taattatttt tcggtgacag cgaatatcgt atggtttttc atattcatac atttttatta
     9361 gggatttata gctgtttaac taagtgtggt taattgcgtg gttaatttga cttatgtaag
     9421 catgattact agtgggatat ttcacaaggt gtaacaagcc ggtgggtaaa gcaccggctt
     9481 gttacaaagt aagcatggga gtttaactgc cccaacgact ttgcagatag ctgaccgctt
     9541 gttgagtctg cggtttattc agatagtcct cacggaacaa gatggtgccg ctaatttctg
     9601 gtaccgcatc gttaagatcg agctgctttt tcagttccgg tacgccgccg ttaatcatcc
     9661 agtctggctc tatctttgaa ggttcaccca ctttatagaa ggcgataccg atatacaggc
     9721 gggtcctggt cggtttaacg acatccgccc accattttgc caacacgtca taacgcgcgg
     9781 cactccgtga gaacggccag taaatttggg gagcaatgta atccagcaat ccttgttcca
     9841 cccatcgacg ggtgtcagcg taggattcgt cataggctgc cgcgcctcgg gtatcggaac
     9901 cgagcggatc gtgtgatcgg ttacgccaca cgcctgccgg gctaacgcca aattcgactc
     9961 ccggcttaat gcttttaatg gtgtgcgata cttttgtaat taactgctga gtattgttgc
    10021 gccgccagtc tgcttttgac gcaaatgcgc ctccgtattt acggtacgtt tcgttatcat
    10081 ttagccgtga acccggtgac tccgtataga aatagtcgtc aaactgcacg ccatctaccg
    10141 gatagcggga aaccacttcg gcgactattg atgtgatcca gtcctgaacc tcagggatgc
    10201 ccgggtcgag gacaaagcga tcgccagacg ttctgatcca gtcacggtgt tgcacataga
    10261 cgctcgccgg ttgttgagac agagtgctat tcagttccct gatagtaccg ggcttcgtat
    10321 taaccgatac gcgatagggg ttaaaccagg cgtgtacttt catcccacgc ttgtgggctt
    10381 cgtcgagcat aaattgcagc ggatcgtaac ccggattttc accaatctta ccggtcataa
    10441 gatcggacca cggcaaaatt tttgatggcc acagggcggt accgtccggc ttgacctgga
    10501 aaaagaccgt gtttataccg agacgttgca gatgatccag tttgtcgatc atcgcctgtt
    10561 gttgtacacg ggcccggctg gtggggttac taatgttaac cgaggaaacc ggtggccagt
    10621 cgaggcgaga caccgtggcc agccagatgc cacgcatcgg ttgtgacgat tgttgcgtcg
    10681 tggctggtgg ctttgaacct gctggtggtg tcaccatgga ctctggaggc gtgcttttac
    10741 aactacacag taaaagtgcc agtgcaacca gtatcgctgg tcttctaatc gttaatttct
    10801 tgtttcggga gcagatatcc atatgtgctg gtttccggta aacagatgtg cgctcattct
    10861 cgtacttatc ccggttaagt caatacgaca gcaagcacga aaaagggagc gatgaattat
    10921 cgctcccttg tcttataacc attcagacat ggttagtgtt tcttgtcatt catcacaata
    10981 tagtgtggtg aacgtgcacg cgggtgcagg aagaagtgac cttttggtgc gttctgactg
    11041 ttgattggct ccagagtgac gccggtattc gctttgcctt tacgatcatg aacagcatag
    11101 agaataaagg gcagggcaag taccaccagg aaactaacga ccagtaattc aacatacata
    11161 tcggtagagt caccctggat gttatccggc ggcaggaagg aaacaataaa cgccataatt
    11221 gaagtcagca gaccgacaat tgccacgacc agttttaccc ctttaccacc agggatatta
    11281 aatgtgcgtt ttaagtcagg atgtttaaga actaacacaa tgtagccaat aaacagcatg
    11341 aaataagcac acagataaat caccaccgtc agcgccagtg cgatcaggaa ggacatgttg
    11401 ttaccgccac cggtattggt gaggatgatc aacgcgatag aagtaatcac cagctgcgaa
    11461 atgaccagcg ttaccggtac gccatttttg ttcattttag cgaatgccgc tggcagcagg
    11521 tttttctgcg ctgtgacata catcccgcga gaaggaccaa caatccagga ggcgatttcc
    11581 gccagaacac ccagcaacag cagtgcggag atcacgcgaa ccgtccactc aatttccggt
    11641 gccacatggg acatcagaac ggtaaaggtt tgcattaccc ctgcggagag gttgatttca
    11701 ttacccggaa tgaccatcgc aatagataaa ccgccaacag agcttaagca gattgccgcc
    11761 accatcagca gtaacatagc cagtggatag tcgcgcccgg ggttgctcat ttcattgacg
    11821 tgagttgcgg aagcttctac gcccatataa ctcaaaatga aggcaacaaa tacaaccagt
    11881 gtgcccactt tagagaagtc agggaagaag gtcttcgaat ccatttcgat agcaacgggg
    11941 gcaccggagt gcagataaat agccgctaat gcgatcaaaa taaatgcagg taacaggata
    12001 ccggcgaaga agccaacttt agcaattcgc gccgtgtatt tcgtgccacc aaactgcgtt
    12061 aatgccagcg cccaaagaat gatgagtgct gcaatagttt tggtaatggg gtcttcattc
    12121 agcgctggcc atttcaggat gtaggagagt gcccctaaca cgaaatagag catcggaata
    12181 aaaccaatgg cgatttgcag atagccaaat gagatcgctg caaatcccca tctcggcccc
    12241 agagtatttg atacccaggc gaagacacca ccttcttccc agccgtcgac ggtggccatt
    12301 tccgcagcac aaagtcccac gggaataaac cataaaatcc cgcctaatag caggaagaag
    12361 actaatgaaa agcccgatgt tgcgaaggta gggtattcat aaacagccat taccatcgat
    12421 gccgttatgg caaagaatcc gagtaatgtg agctgcttag ctttacctgt ctgtactgat
    12481 gtagccatat tatcccccta aaacggtatt cctgtcggaa ccgcacctgt tttgttctga
    12541 agcgtattca gaacaatatt ttccgttgct aatgccagtg aacagacttt ggaaattgtc
    12601 ccgaaacggg ttcgtttcgg gacaccgtta ccgttaaacg ttatcaggta tgtttaaagc
    12661 tgttctgctg ggcaataccc tgcagtttcg ggtgatcgct gagatatttc agggaggctt
    12721 tgtagtcttc cagcaacagt tcagcaaagt ccatttcgaa gccgcgacga cacataat
//
LOCUS       DAJTNN010000116        12735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTNN010000116 DAJTNN010000000
VERSION     DAJTNN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.116"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..470
                     /locus_tag="OO150_002867"
     CDS             <1..470
                     /locus_tag="OO150_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCR5516944.1"
                     /translation="QERPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPD
                     VPEVVSGEDVTQAEQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPP
                     GISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(479..877)
                     /gene="vapC"
                     /locus_tag="OO150_002868"
     CDS             complement(479..877)
                     /gene="vapC"
                     /locus_tag="OO150_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5516945.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(877..1104)
                     /gene="vapB"
                     /locus_tag="OO150_002869"
     CDS             complement(877..1104)
                     /gene="vapB"
                     /locus_tag="OO150_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5516946.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1186..1605
                     /locus_tag="OO150_002870"
     CDS             1186..1605
                     /locus_tag="OO150_002870"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5516947.1"
                     /translation="MMSIAQVRSAGSAGNYYTNKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTDDDGRTVRNGADR"
     gene            1592..6463
                     /gene="traI"
                     /locus_tag="OO150_002871"
     CDS             1592..6463
                     /gene="traI"
                     /locus_tag="OO150_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR5516948.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSYAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRYVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAAARMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYAAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTI
                     RVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVMAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            6483..7229
                     /gene="traX"
                     /locus_tag="OO150_002872"
     CDS             6483..7229
                     /gene="traX"
                     /locus_tag="OO150_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5516949.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7284..7844
                     /locus_tag="OO150_002873"
     CDS             7284..7844
                     /locus_tag="OO150_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5516950.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7975..8187
                     /locus_tag="OO150_002874"
     CDS             7975..8187
                     /locus_tag="OO150_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5516951.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            8431..8892
                     /locus_tag="OO150_002875"
     CDS             8431..8892
                     /locus_tag="OO150_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5516952.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8938..9147
                     /gene="hha"
                     /locus_tag="OO150_002876"
     CDS             8938..9147
                     /gene="hha"
                     /locus_tag="OO150_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5516953.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            9186..9776
                     /locus_tag="OO150_002877"
     CDS             9186..9776
                     /locus_tag="OO150_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5516954.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQTTLLTEREVSFMKGLFRIVDMTRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            10016..10276
                     /gene="repA"
                     /locus_tag="OO150_002878"
     CDS             10016..10276
                     /gene="repA"
                     /locus_tag="OO150_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5516955.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            10500..10574
                     /gene="tap"
                     /locus_tag="OO150_002879"
     CDS             10500..10574
                     /gene="tap"
                     /locus_tag="OO150_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5516956.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10567..11424
                     /gene="repA"
                     /locus_tag="OO150_002880"
     CDS             10567..11424
                     /gene="repA"
                     /locus_tag="OO150_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5516957.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(12477..>12735)
                     /locus_tag="OO150_002881"
     CDS             complement(12477..>12735)
                     /locus_tag="OO150_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5516958.1"
                     /translation="AAAFLGVVPVEKYSGTSVRGCPKLSKIGPPEIRAKLYLASLCGL
                     RFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVLKT"
BASE COUNT         3087 a         3210 c         3780 g         2658 t
ORIGIN      
        1 atcaggaacg accgaaggtc gctccggagt ttattccgcg tgacatcaac ccggaaatgg
       61 agaaccgtct gagtgccgta cttgccgcaa gggaagcaga aggtcgtcag atggccagcc
      121 tcttcgaacc ggatgtcccg gaggttgttt ccggagaaga cgtgactcag gctgaacagc
      181 cgcaacagcc ggtgtctcct gtcatcaacg ataagaagtc agattcaggt gtgaatgttc
      241 cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac
      301 tgccacccgg gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc
      361 agcaggaaaa tcatccggac acctggcagc agatgcagcg tcgtgaagag gtgaacatca
      421 atgtgcaccg ggaacgcggg gaggatgttg agccgggaga tgatttctga tgaacaggtc
      481 agctccagtc ttcagttctc aggccgccca cacgttcaaa ttcccgggtg ttattagtca
      541 caataatcag cccccgactg cgggcatgac ctgcgatcat ttgatcaaat ggcccgacag
      601 ggcgtccctg acgggcaagt tctgctctta tctggccggt gtgtgtggct gcagcagcgt
      661 cgtaatccag aacatcaatg cgggaaacaa atccctcgat cacagcgaga ttacgttcag
      721 gcatctggct tttttctgca ccatatatca gctccatcag ggtgaccgaa ctgatacaca
      781 ttctcccctg gttcaggtta aaacgctccc tgacgctggc gggtttgttc tttatcgtaa
      841 aaatgcagat gttggtatcg agcataaact tcagcatcag aatgactccc tttcctgcat
      901 gccgggctgt tccctgttat ccataaaatc ggcgctgacg ctgtgtccgt cgaaccattc
      961 gtcccatgtc tctccggctg gcgtaatgat tctggttcgt ccgacagcaa tcacttcaac
     1021 gcgttttacg ttttccggca atgcaaccgc ttttggcagt ctgaccgctt ggctgcggtt
     1081 gctgagaaat acggtggttt ccattatttg cctccttatg tggatatgtc ttgtgtatat
     1141 ccattgtggg attgataccg ggatatgtca aagggatata cgtttatgat gagtatcgcg
     1201 caggtcagat cggccggaag tgccggtaat tattatacca acaaggataa ttactatgtg
     1261 ctgggcagca tgggagaacg ctgggccggc cagggggctg aacaactggg gctgcagggc
     1321 agtgtcgata aggatgtctt tacccgcctt ctggagggca ggctgccgga cggggcggat
     1381 ttaagccgca tgcaggatgg cagtaacagg catcgtcccg gctacgacct gaccttctcc
     1441 gctcccaaaa gtgtctccat gatggccatg ctcggggggg acaaacgtct tattgaagca
     1501 cataaccagg ccgtggattt tgctgtccgt catcacccgt gtggaggcac tggcctccac
     1561 acggatgatg acggacggac agtcagaaac ggtgctgacc ggtaatctgg tgatggcact
     1621 gtttaaccac gacaccagtc gcgatcagga accacagtta cacacgcatg cggtggtgac
     1681 taatgttacg cagcataacg gcgaatggaa gacactgagc agtgacaaag tggggaaaac
     1741 ggggttcatt gagaatgtgt acgctaatca gattgccttt ggcaggctct accgggaaaa
     1801 actgaaagag caggttgagg cgctgggcta tgaaactgaa gtggtcggta agcacggtat
     1861 gtgggaaatg ccgggtgtac cggtggaggc cttttccgga cgctcacaga ctatccggga
     1921 ggccgtcggg gaagacgcct cgctgaaatc ccgggatgtg gcggcgctgg atacgcgtaa
     1981 atccaaacag cacgtcgatc ctgaagtcag aatggccgag tggatgcaga cgctgaagga
     2041 aaccgggttc gacatccggg catatcgtga cgcagcggaa caacgggcat atacccgcac
     2101 gcagacaccc ggacccgctt cacaggacgg gccggatgtg cagcaggcgg tgacacaggc
     2161 cattgccgga ttaagtgaac gcaaagtgca gtttatgtat acggacctgc tggccaggac
     2221 ggtcggcata ctgccgccgg aaaatggtgt gattgaacgg gcacgcgccg gtatcgatga
     2281 ggccatcagc cgtgagcagc ttatccccct cgatcgtgag aaggggctgt tcacgtccgg
     2341 gattcatatg ctcgatgagc tgtcagtccg ggcactcagt cgtgacatca tgaaacagaa
     2401 ccgggtgacc gtacatccgg agaaaagtgt cccccggacg gccggttaca gctatgccgt
     2461 cagtgttctg gcacaggacc gtccgtcgct ggccattgtg tccgggcagg gcggtgcagc
     2521 cgggcagcgt gagcgggtgg ctgaactggt catgatggcc cgggagcagg ggcgggaggt
     2581 gcagattatc gctgctgacc gtcgctcgca gatgaacctg aagcaggatg aacggctgtc
     2641 cggtgaactg ataaccggac gtcgtcagtt gctggaaggc atggccttca cgccgggcag
     2701 taccgttatc gtcgaccagg gcgaaaaact ctccctgaaa gagacgttaa ccctgctgga
     2761 tggtgccgca cgtcataacg tacaggtcct gataactgac agcgggcagc gaaccggtac
     2821 aggcagtgca ctgatggcca tgaaggatgc cggggtgaac acatatcgct ggcagggggg
     2881 agaacagcga ccggccacca tcatcagtga accggaccgt tatgtccgct atgcccggct
     2941 ggccggagat tttgcggcca gcgtgaaagc cggagaagag agcgtggcac aggtcagcgg
     3001 agtacgggaa caggccatac tgacacaggc cattcgcagt gagctgaaaa cacagggcgt
     3061 gctcggacac ccggaggtaa ccatgaccgc cctgtcaccg gtctggctgg acagccggag
     3121 ccgttatctg cgggatatgt accgcccggg aatggtgatg gagcagtgga acccggagac
     3181 acgcagtcat gaccgctatg tgacagaacg tgtgacggcg cagagtcaca gcctgaccct
     3241 gcggaatgcg cagggcgaaa cgcaggtggt gcgtatttct tccctggaca gcagctggtc
     3301 gctgttccgg ccggaaaaaa tgccggtggc agacggtgag cgtctgaggg tgacagggaa
     3361 aattcccgga ctccgcgtct ccggcggtga ccgcctgcag gtggcatccg tcagtgaaga
     3421 tgcgatgacg gttgttgtgc cgggacgggc tgaaccggcc accctgcctg tgagcgattc
     3481 accgttcacg gcactgaagc tggagaacgg ctgggtggaa acgcccgggc attccgtcag
     3541 cgacagtgcg acggtttttg cctccgtcac acagatggca atggacaatg ccaccctgaa
     3601 cggtctggcc cgcagtggtc gtgatgtccg gctgtattcc tcactggatg aaacccgtac
     3661 tgcggaaaaa cttgcccgcc atccttcctt tacggtggtt tctgagcaga taaaggcgcg
     3721 tgccggtgag acattgctgg aaaccgctat cagtctgcag aaagccgggc ttcacacgcc
     3781 ggcacagcag gccattcatc tggcccttcc tgtgctggaa agtaaaaacc tggccttcag
     3841 catggtggac ctgctgacag aggcgaagtc gtttgctgca gaaggaaccg gttttgctga
     3901 cctgggaggg gaaatcaatg cgcagataaa acggggtgat ttactgtatg tggatgtggc
     3961 aaaaggctat ggcacaggcc tgctggtttc ccgtgcgtcg tatgaggcag aaaagagcat
     4021 tcttcgccat attctcgaag gtaaggaggc ggtcacgccg ctgatggaga gagtacctgg
     4081 cgaactcatg gagaaactga catcaggaca gcgcgccgcc gcccgtatga tactggaaac
     4141 gtccgaccgt ttcacggtgg tgcagggcta tgccggtgtg ggtaagacca cacagttccg
     4201 ggcggtgatg tcagccgtga acatgctgcc ggagagtgaa cgtccccgtg tcgtggggct
     4261 ggggcccaca caccgtgcgg tcggggagat gcgcagcgcc ggcgtggatg cacagacact
     4321 ggcgtccttt ctgcatgaca cgcagctgca gcagcgcagc ggagaaacgc cggatttcag
     4381 caacacgctg ttccttctcg atgagagctc tatggtgggc aataccgaca tggcacgggc
     4441 atacgccctg attgcggccg gtggcggtcg ggctgtggcc agcggtgaca cggaccagct
     4501 gcaggccatc gcgcccggtc agcctttccg tctccagcag acgcgcagtg ctgccgatgt
     4561 ggtcatcatg aaggagattg tgcgtcagac gccggaactg cgggaggcgg tatacagcct
     4621 gattaaccgg gatgtggaaa gggcgctgtc cgggcttgag cgtgtgaaac cgtctcaggt
     4681 gccacgtctg gagggcgcat gggcaccgga gcactccgtg acggagttca gtcacagcca
     4741 ggaagcgaaa ctggcagaag cgcagcagaa ggcgatgctg aaaggcgagg cttttccgga
     4801 tgtccccatg acactgtatg cagccattgt ccgcgactat accggcagga caccggaagc
     4861 acgggagcag acgctgattg tcacgcacct gaatgaggac cggcgcgtac tgaacagcat
     4921 gattcatgat gcacgggaaa aggccggaga actggggcag gtgcaggtca tggtgcctgt
     4981 cctgaacaca gcgaatatac gcgacgggga gctgcgtcgt ctctccacct gggagaataa
     5041 cccggatgcc cttgccctgg tggataatgt gtatcaccgg attgccggta tcagcaagga
     5101 tgacgggctg ataaccctgc aggatgcgga aggtaacacg cggctgattt cgccccggga
     5161 ggcggtggct gaaggcgtca cgctgtacac cccggacacc atccgggtgg ggactggtga
     5221 caggatacgc ttcacgaaga gtgaccggga gcgcggttat gtggccaaca gcgtctggac
     5281 ggtgacagcg gtttccggtg acagtgtcac actttctgac ggtcagcaga cccgtgtgat
     5341 tcgccccggt caggagcggg cagagcaaca tattgacctg gcctatgcca tcaccgccca
     5401 cggtgcacag ggggcgagtg aaacctttgc catcgcgctt gaaggcacgg aaggtaaccg
     5461 gaaactgatg gccggctttg agtcagccta cgtggccctg tcgcgtatga agcagcatgt
     5521 gcaggtgtac accgataacc gtcagggctg gacggatgcc atcaacaatg ccgtacagaa
     5581 aggaactgcc cacgatgtgt ttgagccgaa accggaccgg gaggtcatga atgcagagcg
     5641 gctgttcagt acggcgcggg agctgcggga cgtggcggca ggccgtgccg ttctccgtca
     5701 ggcggggctg gccgggggag acagtcctgc acggtttatt gctccgggac gtaaatatcc
     5761 gcaaccgtat gtggcactgc cggcgtttga ccgtaacggc aagtccgccg gtatctggct
     5821 gaacccgctg accacggatg acggaaacgg gctgcgggga ttcagcggtg aaggtcgggt
     5881 aaaaggcagc ggggatgcgc agttcgtggc cctgcagggc agccgtaacg gagaaagcct
     5941 gctggctgat aatatgcagg atggcgtccg gattgcccgt gataatcctg acagtggtgt
     6001 ggtggtaaga atcgccggtg aaggccgtcc gtggaatccc ggtgccatca ccggtggccg
     6061 cgtgtggggg gatatcccgg acaacagcgt ccagccggga gccggaaatg gcgaaccggt
     6121 catggcagag gtactggcac agcggcaggc tgaagaggcc atccgccgtg aaacggaacg
     6181 tcgcgcagat gaaattgtcc gtaaaatggc agagaacaaa cctgacctgc cggacggcaa
     6241 aacagagcag gctgtcaggg aaattgccgg gcaggagcgt gaccgggctg ccataactga
     6301 acgggaagcc gcgctgccgg agagtgtact gcgtgaacca caacgggtgc gggaagcggt
     6361 ccgggaagtt gcccgggaaa atttgctgca ggagcgtctg cagcagatgg agcgggatat
     6421 ggttcgtgac ctgcagaaag agaaaacccc gggcggagac tgatacagga agacaaacac
     6481 tgatgacaac ggataacacg aacacgacac gtaacgattc actggctgca cggaccgata
     6541 cctggttgca gtcatttctg gtctggtcac ccggacagcg ggatatcatc aaaacggtgg
     6601 cactggtgct gatggtactg gaccacatca atctgatatt ccagctgaag caggaatgga
     6661 tgtttctggc cgggcgagga gcctttccgc tttttgccct ggtgtggggg ctgaatctgt
     6721 cccgtcatgc gcatatccgg caaccggcca ttaaccggct gtggggatgg ggaattattg
     6781 cccagttcgc atattatctg gcaggctttc cctggtatga ggggaatatc ctgtttgcct
     6841 ttgcagtggc ggcacaggtg ctgacgtggt gtgaaacgcg cagtgggtgg cgtacagcag
     6901 cggccatact cctgatggcc ctgtgggggc ctttgtccgg caccagttac ggtattgccg
     6961 ggttgctgat gctggcagtc agcaaccggc tgtaccgggc ggaagacaga gcggaacgtc
     7021 tggcgctggt cgcctgcctg ctggctgtta ttccggcgct taaccttgcc accagtgatg
     7081 cagcggcggt ggccggtctt gtgatgacgg tgctgaccgt tggtctggtg ttgtgtgccg
     7141 gaaaatcatt accccgtttc tggcccggag actttttccc gacgttctat gcctgtcatc
     7201 tggctgtgct gggcgttctg gcgctgtgac gggtgtggta tctttggccg caagaggatg
     7261 attcgtcaga ggcagaacac agcatgacag agcagaagcg accggtactg acactgaagc
     7321 ggaagacaga gggaacagcg actgtccgca gccggaaaac catcatcaat gtcaccacgc
     7381 caccaaagtg gaaggtgaaa aagcagaagc tggccgagaa agcggcccgt gaagcagagc
     7441 tggcggcaaa aaaagcgcag gccagacagg cgctgtccat ttatctgaac ctgcccacgc
     7501 tggatgaggc tgtgaatacc ctgaagccct ggtggccggg attatttgac ggtgacacgc
     7561 cccggcttct ggcctgcggt atccgggacg tgttactgga agacgtggcg cagcggaata
     7621 tcccgctctc gcataaaaaa ctgcgcaggg cgctgaaggc catcacccgt tcagaaagct
     7681 atctgtgtgc catgaaagcc ggtgcctgcc ggtatgacac ggaagggtat gtgacggagc
     7741 atatttctca ggaggaggaa gcgtatgcgg gagcgcgtct ggcgaaaatt cgtcgccaga
     7801 accggataaa ggcagaactt caggccgtgc ttgatgagaa ataaaaaaag cctcccccag
     7861 agagaggagg cagggaaata aggcttaaaa ggaaataact tccgcttccg aaaggaagcc
     7921 agtccactga attatttatc acaataatac tgacaactaa agggggacaa gaaaatgaat
     7981 ggattcagaa acagttcacg gaacggccag gtctggcgtt accagcgtgc cggcgggcgg
     8041 gccgttattc tggaagtcag tggacgctgg atggaagcgg cagaagcatg gcgacgggct
     8101 gcctgtgttg cgcctaggac agactggcag cagtttgccc ggaaaagggc tgaacactgc
     8161 caccggcgct gccggggaag ggggtaacga aaaaagcagc cttcattact ccaggagtca
     8221 gaaggctgaa aggagtttat gagatttgct tcttaatatc cggtcgggca gtatgaccgg
     8281 acagttctga gtagcacaga gtcttttccg cattaagtcg attttttata ttttgtgatt
     8341 aatttcacaa aataaggtgt tattcaatgt gtgctgcact attcaggggg gctgaaatac
     8401 aggtgccatt tatctgatat ggagaataac atgaggaaat atattccact ggtattattt
     8461 atcttttcat ggccggtatt gtctgcagat attcatggac gggttgttcg tgtactggac
     8521 ggtgatacca tcgaggtcat ggactcactg aaggcggtgc ggatcaggct ggtaaatatt
     8581 gatgcaccgg aaaagaaaca ggattatggc cggtggtcaa cagacatgat gaaatcactg
     8641 gttgcaggaa aaacggttac cgtcacatat tttcagcgag atcgttatgg tcgtattctc
     8701 gggcaggttt atgcaccaga cggaatgaat attaatcagt ttatggtccg ggccggggct
     8761 gcctgggtat atgagcagta caacacagat cccgtgctac ctgtccttca gaacgaagcc
     8821 agacaacaaa aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggagg
     8881 caccgtaaat aaagcgatgg caatcctatc attcatctgt tctcagcggg aataaaaatg
     8941 gcaaaaacaa aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg
     9001 gaaaaaatta ttcataaaaa cagagacagt ttatcaaaca gtgaacgtga atcatttaat
     9061 tcagcagcag accaccggct agcggaatta atcaccggaa agttatatga caggatacca
     9121 aaagagatat ggaaatatgt aaggtaataa ttcagcaata attaattgcc aggagcagat
     9181 aaataatgaa gttgattatt tttattctca ttgttctgat tattgcagcg ctacttatcc
     9241 ggattatcct cagaagtgta aatcagcatt ctcctttgct gatgcaattg catgcggcag
     9301 gtatacggac cggtgatgca gagcgaatac tgtccagcgg tgaatactgg caacgtcaga
     9361 cgacgctgct gacagaaagg gaagtcagtt ttatgaaagg actgttcaga attgtggata
     9421 tgacgcggtg gtatctgtgt ccgcaggtac gggtcgcgga tatcgtccag ctgaacggga
     9481 atatccggcc acgatcacgc cagtggtggc agttattcag gatggtgtct cagtggcatg
     9541 ttgatgtggt tatcgttgag ctgcgttcgt tcagtattgt tgcagcagta gagctggatg
     9601 atgccagcca tttacgaccg gaacgcagac gccgggatat tcttctggaa gaggttctga
     9661 ggcaggctgg tattccgttg ctcagaagcc acgatgccag aaaactgctg cagatgaccg
     9721 gagaatggct gaatacaaca ggggctgatc agcagtcacc ggaacatcgt agctgacgcc
     9781 ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa gcaagttttc ctcgctcccg
     9841 gcgtttccat aactgaaaac catactattt cacagtttaa atcacattaa acgacagtaa
     9901 tccccgttga tttgtgcgcc aacacagatc ttcgccacaa ttctcaagtc gctgatttca
     9961 aaaaactgta gtatcctctg cgaaacgatc cctgtttgag tattgaggag gcgagatgtc
    10021 gcagacagaa aatgcagtga cttcctcatt gagtcaaaag cggtttgtgc gcagaggtaa
    10081 gcctatgact gactctgaga aacaaatggc cgctgttgca agaaaacgtc ttacacacaa
    10141 agagataaaa gtttttgtca aaaatcctct gaaagatctc atggttgagt actgcgagag
    10201 agaggggata acacaggctc agttcgttga gaaaatcatc aaagatgaac tgcagagact
    10261 ggatatacta aagtaaagac tttactttgt ggcgtagcat gctagattac tgatcgttta
    10321 aggaatttta tggctggcca cgccataagg tggcagggaa ctggttctga tgtagatgta
    10381 caggagccag aaaagcaaaa accccgataa tcttctttaa ctttggcgag tcagaaagat
    10441 taccggggcc cacttaaacc gtatagccaa caatcaagct atgcggggag tatagttata
    10501 tgcccggaaa agttcaagac ttctttctgt gctcgctcct tctgcgcatt gtaagtgcag
    10561 gatggtgtga ctgatcttca acaaacttat taccgccagg taaagaaccc gaatccggtg
    10621 ttcacacccc gtgaaggtgc ccggacgctt ccgttctgcg ggaaactgat ggaaaaggcg
    10681 gtgggcttca cctcccgttt tgatttcgcc attcatgtgg cacatgcccg ttcgcttggt
    10741 ctgcgtcggc gcatgccacc ggtactacgt cgccgggcca ttgatgcgct gctgcagggg
    10801 ctgtgttttc actatgaccc actggccaac cgcgtccagt gttccatcac cacgctggcc
    10861 attgagtgcg gactggcgac ggagtctgct gccggaaaac tctccatcac ccgggccacc
    10921 cgggccctga cgttccttgc agagctggga ctgattacct accagacgga atatgacccg
    10981 cttatcgggt gctacattcc gaccgatatc acgttcacat ctgcactgtt tgctgccctc
    11041 gatgtatcag aggaggcagt ggccgccgcg cgccgcagtc gtgtggaatg ggaaaacaga
    11101 cagcgcaaaa agcaggggct ggataccctg ggtatggatg aactgatggc gaaagcctgg
    11161 cgttttgttc gtgagcgttt ccgcagttac cagacagagc ttaagtcccg tggaatgaag
    11221 cgtgcccgtg cgcgtcgtga tgcggacagg cagcgtcagg atattgtcac cctggtgaaa
    11281 cggcaactga cgcgtgaaat ctcggaaggg cgcttcactg ccagtcgtga ggcggtaaaa
    11341 cgtgaagtgg agcgtcgtgt gaaggagcgc atgattctgt cacgtaaccg taattacagc
    11401 cggctggcca cagcttcccc ctgaaagtga cctcctcaga ataatccggc acgcgccgga
    11461 ggcattcgca cgcctgaagc ccgccagtgc acaaaaaatc agcgctacat acaaaaaaca
    11521 acctcatcat ccaccttctg gtgcatccgg tcccccctgt ttttgataca aaacacgcct
    11581 cacagacggg gaattttgct tatccacatt aaactgcaag ggacttcacc ataaagttac
    11641 aaccgttcac gtcataaagc gccagccgtc agccctacag ggtgcaatgt atcttttaaa
    11701 cacctgttta tatctccttt aaactactta attacattca tttaaaaaga aaacctgttc
    11761 actgcctgtc ctgtggacag acagatatgc acctcccacc acaagcggcg ggccccgacc
    11821 gaagccggtt tagttacaac atgcaaacat aaacccctaa aaaacccaag tccgacgcag
    11881 aactgaaacc acaaagcccc tccctcataa ctgaaaagcg gccccgcccc ggcccgaagg
    11941 gccggaacag agtcgctttt aattatgaat gttgtaacta tattatcatc gctgtcagtc
    12001 ctctggctgg acgtatcgag tacacgctcg taagcggccc tcacggccct cacggcccgc
    12061 taacgcggag atacgccccg actgcgggta aacccttgtc gggctccgac cgcgcacaga
    12121 agctctgtca tggctgaaaa cgggtatggc ttggcagcta ccatgttctg tcaagaaaaa
    12181 tcgcttgaac tccgacgtac agccccctaa gtactgccag caagctgtca gcactaagtc
    12241 cccgatacag cgcggcgtag ccgcgctaac ggggttaaaa gggcttacct atacttcatc
    12301 atcgctgaaa gcccgcatgg cctcgcaggg cagagatggg ataggtgaaa cctatcaata
    12361 cgtaccggct tacgccgggc ttcggcggtg ttactcctgt accatatgta actacatcat
    12421 gccgccttac atgcgctacg cgcatatgaa gacaaatagc ccacatcaaa cggtttttag
    12481 gtcttcagta cgccatagca ccagtgcacg agttttctca tcagcgcacc aatggcacac
    12541 attttggcct tacctctcag gcataatcgc tcatacatcg ctttcatcaa tgggttaaat
    12601 ctcagaccac acaacgaagc cagatacagt ttcgcccgga tttccggcgg accaattttt
    12661 gacagtttcg gacaccctct gaccgaggtg ccggaatatt tttcaacggg aacgacacct
    12721 aaaaatgcag ccgcc
//
LOCUS       DAJTNN010000117        12718 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTNN010000117 DAJTNN010000000
VERSION     DAJTNN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.117"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            6..1634
                     /locus_tag="OO150_002882"
     CDS             6..1634
                     /locus_tag="OO150_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR5516959.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKQSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVKLAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1873..1932
                     /locus_tag="OO150_002883"
     CDS             1873..1932
                     /locus_tag="OO150_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5516960.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(2052..3485)
                     /gene="hldE"
                     /locus_tag="OO150_002884"
     CDS             complement(2052..3485)
                     /gene="hldE"
                     /locus_tag="OO150_002884"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5516961.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3533..6373)
                     /gene="glnE"
                     /locus_tag="OO150_002885"
     CDS             complement(3533..6373)
                     /gene="glnE"
                     /locus_tag="OO150_002885"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5516962.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMMLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6396..7697)
                     /gene="ygiF"
                     /locus_tag="OO150_002886"
     CDS             complement(6396..7697)
                     /gene="ygiF"
                     /locus_tag="OO150_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5516963.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7939..8559
                     /locus_tag="OO150_002887"
     CDS             7939..8559
                     /locus_tag="OO150_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5516964.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKERWMN"
     gene            8623..9861
                     /gene="cca"
                     /locus_tag="OO150_002888"
     CDS             8623..9861
                     /gene="cca"
                     /locus_tag="OO150_002888"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5516965.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10018..10839)
                     /gene="bacA"
                     /locus_tag="OO150_002889"
     CDS             complement(10018..10839)
                     /gene="bacA"
                     /locus_tag="OO150_002889"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5516966.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10930..11298)
                     /gene="folB"
                     /locus_tag="OO150_002890"
     CDS             complement(10930..11298)
                     /gene="folB"
                     /locus_tag="OO150_002890"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5516967.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11403..12020
                     /gene="plsY"
                     /locus_tag="OO150_002891"
     CDS             11403..12020
                     /gene="plsY"
                     /locus_tag="OO150_002891"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5516968.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12033..>12718)
                     /gene="ttdR"
                     /locus_tag="OO150_002892"
                     /gene_synonym="dan"
     CDS             complement(12033..>12718)
                     /gene="ttdR"
                     /locus_tag="OO150_002892"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCR5516969.1"
                     /translation="VDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQV
                     HFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQSL
                     QELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIML
                     RSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLVAWCQQRLG
                     KPDEGYQVM"
BASE COUNT         3065 a         3600 c         3177 g         2876 t
ORIGIN      
        1 cctggatgtt taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg
       61 ctaggctcta tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc
      121 aaaaagtggt gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca
      181 tcaatatgaa tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga
      241 aagatcgtat gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa cagtctgtag
      301 aaggcatcgc cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac
      361 gtatgttggt tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca
      421 tgcatgagtt acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag
      481 aagacctgtc gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga
      541 cctcgaaaga acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga
      601 ttcaggagac ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg
      661 cggtgcgtga gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag
      721 cgtttatgac gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac
      781 gtattgcggc ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg
      841 aacgacaaat tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg
      901 aggctgaacg tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca
      961 accagacgaa atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc
     1021 gtgctaacct tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc
     1081 agaccgcaga agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag
     1141 aaaccaaagc ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc
     1201 aggcggcggc tatcgttaag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag
     1261 aagcacaacg tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta
     1321 aattcaaact ggccttgttg caggcgctac ctgcggtaat agaaaaatcc gttgagccga
     1381 tgaaatctat tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg
     1441 cgggtgatgc aaacacgggt aatgtggggg gcggaaacct ggcggaacaa gcattatcag
     1501 ccgctctctc ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg
     1561 tttcaggcgg ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg
     1621 aaaacgtaga ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt
     1681 atatctgcgt tccgctaaaa actgcaaatg atcaggtcgt tgcaacgttt tgcgtgaccg
     1741 ctcggggaag gcaaaactgc ctctgggaaa gcgttgctcg aggtccggcg ctcatcaaca
     1801 atcggggagt cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag
     1861 aaggaaaggg ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc
     1921 gcagcttatt aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac
     1981 tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga gatctgtgaa
     2041 ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt agtggtcgag
     2101 caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac ttctttactc
     2161 ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg caggatcccg
     2221 gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac cgcttccagt
     2281 gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc ccctttcagc
     2341 cgtttggtgg aggcatcgct gttcacagcg acaatcagac ggtcacccag tttgcgagca
     2401 tttgccagat aggagacgtg cccggcatgc aggatgtcaa agacaccgtt ggtcattacc
     2461 accttttcgc cgcgtttacg cgctgccgct acggccagct tcagttcctc ttcggtcatc
     2521 acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat cggcgaaacc
     2581 gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa gaagcaggct
     2641 tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc
     2701 gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg cgctttaccc
     2761 ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga gagttcgtaa
     2821 tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca tttaccgaca
     2881 acagcttcaa attccgagag attcggcgtt aacagcgtag cgccgcggta gcgctcaaaa
     2941 tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag ttggatcatc
     3001 tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc gccaatcgaa
     3061 ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacgccttc gaaaccttct
     3121 tcgaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt ggtgatggtc
     3181 ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag agatttactc
     3241 agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga
     3301 gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat ggtattcact
     3361 ttaaccacgg gcaccggcgc ttccggcgag atacggctgg tgggaccgta ccagtaacga
     3421 tccagcatta catcaccaac caccatcact cctgcacgtt caaactctga cagcgttact
     3481 ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata cttcattctt
     3541 ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg aagcaatcct
     3601 ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg
     3661 tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt tgcgccagca
     3721 gctccagaat acgcacgttg tctgaccagc gcgttaactt cggtttttca tgagcgtagc
     3781 gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca tcggctttga
     3841 tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg cgcatttccc
     3901 gcacttccgt ttgcagagtt ttaccttcac gcggcagcat cataatctcg cgacgcactg
     3961 cgtcaaagtg cgcggtgagc tgcggatcgc cgtacactac acgcgcacgc accagcgcct
     4021 gatgctccca cgtccaggcc tcgtttttct gataatcagc aaatgcttct gcggatgtca
     4081 ccagcattcc cgccgccccg gacggacgca gtcgtgcatc cacttcatac aaaatgccgg
     4141 aagaggttcg cgtattgaac agatgcataa tgcgttgcgc cagacgcaga taaaactgtc
     4201 gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca tggaggaaga
     4261 tcaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg tagccgacca
     4321 ccgcaaaacc acggccttcg cgatcgttga ggtgatttgg cttaccgtag cgggccacca
     4381 tttgaaccca cgcctgctga acgacggcat ctatcatagc ctctgccagc caggttaagt
     4441 gatcgctcac tttcatcacc ggtagcgtac cagcgatatc cgctgcggcg atgcgtaaca
     4501 gctgcgcctg tttgaactga cgcagcgcct caagctgttg ctcttcgtca tcttccggca
     4561 cgcgcagcaa atactggcgc aactcatcgc ggtaggcatc ggtcgccgtc ggctggtaaa
     4621 gagtgtttgg atcgagcaat tcatccagca ataatggata acgcgccagc tggctggcaa
     4681 tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg aattcactga
     4741 gcaattctaa ataggtggtg cgggtaacaa tccccaccag caaggcggta atgcgcgaca
     4801 gcgtaacggc agcgtcttca cgcgcacaga catcacttag cagatgtggc atcagatggt
     4861 cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg cggaaatcag
     4921 caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc actggcgtag
     4981 tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac agcgactctt
     5041 cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc acattggtca
     5101 tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc gcccacgcca
     5161 gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg ttaatgcttt
     5221 gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt tcagcatcgt
     5281 tttcagaaag cagatgcagc gcggcaatga cgctgagcgt tggtagtaaa gagcgcgact
     5341 gcagcgacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata aattcaattt
     5401 cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga cgcacttcac
     5461 gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac
     5521 ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct tcgctatcgc
     5581 ccataatccg cgccttgacc atcgcgtaac gctcccagtc gcgcccctgc tcctggtaat
     5641 aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac ggacgcagcc
     5701 gcatatccac gcgatagacg aagccatcct gcgttggttg atccaacact ttaatcagcc
     5761 gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg ccctgcgtac
     5821 aaccatgttc cggccaggca aaaatcagat cgatatctga ggagaaattc agttccccac
     5881 cgcccagctt acccatgcct aaaatcagca gcggttgcgc ttcgccctgc gcattgcacg
     5941 gcgttcccca ctcgcggcag caagcgtcat acagccagtc acgcgcggcg acaatcagcg
     6001 tctccgccag atggctgagc tgctgcaata tactctcttc agtaaccaaa gccagcgttt
     6061 gcgcccaggc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg cgcattaacc
     6121 cggcttcgtc actcacattg ctgagcgcct cctgcaacca tgccgcgtaa tgctgccatt
     6181 catcagcctg cggcgattgg ctttccagtt ccgtcagcca ctctggatgc gcaatcacgc
     6241 tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcgcta agtgatttct
     6301 cggctaaagg ctctggcagc cgctcaacaa cggtctgcca gtactgctgt aacggtgaag
     6361 agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct gtgcaaccag
     6421 aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg ttgcccggtc
     6481 gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac gacaggatca
     6541 tagtaacccg ccagcaacag tattgagtca atatcacgcg tcagacgtgg caactggtca
     6601 cggtagcgat cgcccaacgg ctggcagaaa acgcttttca gttcagcggc atggcgggaa
     6661 agatggatat cggcaaagcg tttgaaggaa tcgctcattt tactctgcgc tttggcatct
     6721 aaaaacggct gccaggcttt gcttaccagc cattcggtca acgccagctt cgccattgcc
     6781 gttttggtag agtagaccgc cgtcacggca gaaaccgcag aagcgatggt cgcttcgcat
     6841 tgagtcagca aatcgcgtaa gtgagtgctc gctttgcgcg gcacaatacc accgaacagc
     6901 atcagtgtat gacggaccag accaatggct gccagcacct gttctttcgc cgcatcgttg
     6961 ccgcgtaccc acagttcttc atgatactgc cattgcgcta acgccagctc gagcgccgct
     7021 tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt cggtttgatt
     7081 tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct caggctgccc
     7141 tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg
     7201 ccgctaagca gttccagttc cagctcacag ataggttcag caaattcccc cgctttcacc
     7261 tccccctgat cgagagcgat ttcaattcgg ctgtcatcga ccgccaccag ccatttttcg
     7321 cgataaaaat cggtgctgaa cagcggttgt acgcgggaag cgagatcggc gggcaattcg
     7381 ccgttcggcc agacttccgt cggtaactgc gccaggtcga gcgtcggttc gctcaacgcc
     7441 acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc
     7501 atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgccc acgcagccag
     7561 ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg ctcgccgccc
     7621 agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat aaactttaat
     7681 tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga cgatgaacgg
     7741 cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt gccatgcaac
     7801 gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca gaggattccg
     7861 attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc cactttccat
     7921 gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt actcgcactt
     7981 agcgccactg ccgtctcaca cgctgaagaa acgcgctatg tttctgatga actgaatacc
     8041 tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa cgccggcgag
     8101 gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa agacagctct
     8161 ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagcct gcgctcccgt
     8221 gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa tatcgataac
     8281 acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc
     8341 aacggattaa aagaagaaaa tcagaaattg aaaaacgagc tgattgtcgc gcagaaaaag
     8401 gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat gcaatggttt
     8461 atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact gccacacctg
     8521 atcccaacgc gcaaacgcaa agagcgctgg atgaactaaa tcgccttctt cgttgcactg
     8581 tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat ttatctggtc
     8641 ggtggtgctg ttcgggatgc attgttaggg ctaccggtca aagacagaga ttgggtggtg
     8701 gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg ccgcgatttt
     8761 cctgtgtttc tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac cgaacggaaa
     8821 tccggttccg gttacaccgg ttttacttgc tatgccgcac cggatgtcac gctggaagat
     8881 gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa cggcgagatt
     8941 atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc
     9001 gcttttggcg aagatccgtt acgcgttctg cgcgtggcgc gttttgctgc gcgttatgcc
     9061 cacctcggtt ttcgtattgc cgatgaaact ctgacgctga tgcgcgagat gacccatgcg
     9121 ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag cgccctcacc
     9181 acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggtgcact tcgcgtttta
     9241 ttcccggaaa ttgacgccct gtttggcgtt ccggcccctg ccaggtggca tccggaaatc
     9301 gacaccggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag tccgcaggtt
     9361 gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc gccagaactt
     9421 tggccgcgtc atcatgggca tggcccggcg ggtgttaagt tagtggaaca actatgccag
     9481 cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga gtttcacgat
     9541 ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattatt tgattccatc
     9601 gatgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga ggctgacgta
     9661 cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgttggtt gcgcgaagcc
     9721 tgggaagtgg cgcagtcagt gccgacaaaa gcggtcgttg aagcgggatt taaaggtgtg
     9781 gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagttg gaaggaacaa
     9841 cgttgcccaa agcctgactg agtataaaac taaacgttaa cacgaaccat cccctcgccc
     9901 cttcggggag agggtgaggg gaacagacca gcaccggtgc gaacaatcac ccccaccccg
     9961 gccctctccc tacagagcga gggagaaaac caggcctgct cccgaacctt ttctcaatca
    10021 aaagaacacg acgtacaccg cagccgccac aataaagcga taaatggcga acgggataaa
    10081 cgaaatgcgc ttaatcaatt gcaggaaggt tttaatcgct atcagcgcca ccacaaaagc
    10141 ggtgataaac ccaacggcaa acatcgggat atcgccggtt gtcaggaagc cccagctttt
    10201 gtagagatcg agcgccgttg cgcccatcat catcggtacc gccagcagga acgagaactc
    10261 ggaagcagcg taacggctca cgcccatcag catcccacct gaaatggtgg ccccggaacg
    10321 ggagaaaccc ggccacagcg ccagacactg gaaacagcca atcataaacg cctgacgata
    10381 ggtcatatca tcaagacccg gcgcacgcgg ctctttcggc ttcaggcatt cggcggcaat
    10441 cagcaacaaa ccgccaacga ccagtgcata catcacattt atcgggttaa acaatgactt
    10501 aatcgtgtcg tggaacaaca gccccagtac caccgccgga atcatcccca gcaaaatgtg
    10561 gatcagcgtt aaacgacctt tgctttctcc ttcgtgctgc aacgggcggc caaagtggat
    10621 gccaatcagg ccaaacagac gccgccagaa catcactact accgccagaa ttgatcctaa
    10681 ctggatcaca acttcaaagg ttttcgccgt gtcgccctcg aaccccaaca agtgaccgac
    10741 aataatcata tggcccgtgc tggataccgg cagaaattct gtcaatcctt cgaccacacc
    10801 caatattgcc gctatcagca gcgagtgcat atcgctcatc aataaacccc taaattatta
    10861 aaatgtaccg cttgtccgga ctactgcagt aagaccaggt tataaccgtt tggtttaaca
    10921 gctgtaaaat taattatttt ctttcagatt attgccacgc tcaatgatta caccaacatt
    10981 cgccgcccgc gccactgcgc ctggcttgct gagtttgatg cgcacccacg gcgagttgaa
    11041 gcgtgctaac agcagctccg ccacctcttc agccacgcgt tccaccagcg caaaacgcgc
    11101 cccctcgacg tggctgacca ccgtttctgc aatgtcagcg taactgaggc aatccgccac
    11161 atcatcactt ttcgccgctt tacggttatc ccatgccatt tcgatatcga acactaactt
    11221 ctgttcgatg gtctgttccc agtcgtaaac accaatagtg gtgattaccg aaagttgctc
    11281 tataaataca atatccatca cgtcctgcct gctttttggc taaccggata ccacttccgg
    11341 cgaaatgtgc gtattatcca cagattcatc gttgaacacg aattttcaaa acggaacagc
    11401 ttatgagtgc aatcgcgcct ggaatgatcc tcatcgcgta cctctgcggc tccatttcca
    11461 gtgccattct ggtttgccgc ttgtgtgggc tgcccgatcc gcgaaccagc ggctccggca
    11521 atccaggcgc aaccaatgtg ttacgtatcg gtggcaaggg agcagccgta gcagtactga
    11581 ttttcgacgt tctgaaagga atgttgcccg tctggggcgc gtatgaatta ggtgtcagcc
    11641 ccttctggct aggcttaatt gccatcgccg cctgtcttgg acacatctgg cccgttttct
    11701 tcggatttaa aggaggaaaa ggcgttgcta ccgcttttgg tgccatcgca cccattggct
    11761 gggatctcac cggagtaatg gcgggaacct ggttactgac cgtgctattg agcggatact
    11821 cgtcgctggg agcgattgtc agtgcactga ttgctccgtt ttatgtctgg tggtttaagc
    11881 cacaattcac cttcccagtt tcgatgctct cttgcctgat cctgctgcgt catcatgaca
    11941 acatccagcg tctgtggcgt cgtcaggaga caaaaatctg gacgaaattc aaaagaaagc
    12001 gcgaaaagga tcccgagtga tttctggtgg atctacatga cctgatagcc ttcatcgggc
    12061 ttgcccagcc gttgctggca ccatgccacc agaaattcca cgcagacacg taatttcatg
    12121 ctgcgataga gcggctcccg gtaaacagcc cagatattgg cgctttgtgc atactccggc
    12181 aatacttgca ccagtttgcc actctccaga aacggcaaca catcccactc ggaacgcagc
    12241 ataatccctt tgccctccag cgcccattgc aacactatct cgccgctatt ggaggaaaga
    12301 tgcccgctaa ccttcaccga ttttttctcc tgaccgttcc ccaactccca tattccgtgg
    12361 gtcatatcgc gctctttggt caccagacag tcatgacgac ttaattcctg taatgattgt
    12421 ggctgcggat atttttgcag atactcaggt gctgcgcata atattctttt atttttcgtt
    12481 aacagatgcg caatataata atcaggaatt tcgtcattaa tacgaatatc cagatcaata
    12541 ttatcctgca ctaaatcaat ttgccgatcg aacaattcaa aatgcacctg taactcagga
    12601 taattgcgca taagttcggt aatggctggc gcaatatggc tgcgcccaaa accaaaactg
    12661 caaccaatac gaatcatccc ctccgggcgc gttttgattt gcgtgacgtc atcgacca
//
LOCUS       DAJTNN010000118        12707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTNN010000118 DAJTNN010000000
VERSION     DAJTNN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.118"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..666)
                     /gene="rarD"
                     /locus_tag="OO150_002893"
     CDS             complement(<1..666)
                     /gene="rarD"
                     /locus_tag="OO150_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5516970.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMLLNLLLIA
                     AGIV"
     gene            complement(718..1191)
                     /locus_tag="OO150_002894"
     CDS             complement(718..1191)
                     /locus_tag="OO150_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5516971.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATATYMVG"
     gene            1356..2225
                     /gene="pldA"
                     /locus_tag="OO150_002895"
     CDS             1356..2225
                     /gene="pldA"
                     /locus_tag="OO150_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5516972.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2358..4187
                     /gene="recQ"
                     /locus_tag="OO150_002896"
     CDS             2358..4187
                     /gene="recQ"
                     /locus_tag="OO150_002896"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5516973.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMCKLERFGKPFMALIRAHVDGDDEE"
     gene            4251..4871
                     /gene="rhtC"
                     /locus_tag="OO150_002897"
     CDS             4251..4871
                     /gene="rhtC"
                     /locus_tag="OO150_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5516974.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(4933..5553)
                     /gene="rhtB"
                     /locus_tag="OO150_002898"
     CDS             complement(4933..5553)
                     /gene="rhtB"
                     /locus_tag="OO150_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5516975.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5664..6686
                     /gene="pldB"
                     /locus_tag="OO150_002899"
     CDS             5664..6686
                     /gene="pldB"
                     /locus_tag="OO150_002899"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5516976.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6694..7494
                     /gene="yigL"
                     /locus_tag="OO150_002900"
     CDS             6694..7494
                     /gene="yigL"
                     /locus_tag="OO150_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5516977.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7570..8469
                     /gene="bioP"
                     /locus_tag="OO150_002901"
     CDS             7570..8469
                     /gene="bioP"
                     /locus_tag="OO150_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5516978.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTLITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8357..9310)
                     /gene="metR"
                     /locus_tag="OO150_002902"
     CDS             complement(8357..9310)
                     /gene="metR"
                     /locus_tag="OO150_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5516979.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9546..11807
                     /gene="metE"
                     /locus_tag="OO150_002903"
     CDS             9546..11807
                     /gene="metE"
                     /locus_tag="OO150_002903"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5516980.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12092..12337)
                     /locus_tag="OO150_002904"
     CDS             complement(12092..12337)
                     /locus_tag="OO150_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5516981.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12327..>12707)
                     /locus_tag="OO150_002905"
     CDS             complement(12327..>12707)
                     /locus_tag="OO150_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5516982.1"
                     /translation="PFNPDLAGGPIENLTTDGVTINREGIAIVEKHIARFGHDPVNEV
                     MINRLKDIEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPDGDAGYALWNHTHTA
                     TLEDYKLKGELNDLYHQDALDYDN"
BASE COUNT         2986 a         3204 c         3396 g         3121 t
ORIGIN      
        1 gacaataccg gcggcgatca gcagcaaatt cagcaacatc gggttttgcc ccatatggct
       61 ggttgagctg tcggcaatag caaacagata aatcgccgcc acgggcagca gccacatggt
      121 ttcgattaac atgccggttt gcgcttcaac ggcaattttc ttgcgtacca gtccgtagaa
      181 ggcaaaacta aacgccagcc ccagcgcgat gattggcagc gaaccaaaag tccacaactg
      241 aaccaacacg ccacatatcg ccagaatcac cgccagccat tgcatccggc ggaatcgctc
      301 gccgaggaaa atcatcccca gcacaatgtt caccagcggg ttaataaagt aaccaaggct
      361 cgcttccagc atatggtgat tgttcaccgc ccagataaac agtagccagt tgccaccaat
      421 cagcacggca gagactgcca gcataaaaat tttctgtggc gtctgaatca gcgtttttaa
      481 ataggaccac tggcggcaaa tgctcatcag caccaccata aagaaaaacg accagatcac
      541 gcgatgcgtc aggatctcat cggcgggcac gtagtaaatc agcttgaagt atgctggcgc
      601 aataccccaa ataaaataag cggcaagagc gagtaatacg ccctgccgcg tttgttttgc
      661 atccatcggg aatactcatt tttaattggt aacagcagtt tacctgcttt tatgtcttca
      721 acctaccata taggtggcgg tggcggtggc actggcaata taaagctgtt cttcattgtg
      781 taattcaacg cgggcgacgg cgactttatt gcctgcacgc aacaggctac tggtagcagt
      841 aaaacgctcg cccctgcctg ggcgcagata atcaacgcga agatcaatgg tccccatccg
      901 cgatagccgc tggcgtagtt catcttcact gatggtttcg tggcgggtta aggtacttcc
      961 cacgcacacc agaccggcgg cgacatccag cgccgacgca atgactccgc cgtgcaaaat
     1021 gctttgcgcc cagttaccca ccatcatcgg ctgattttta aaggccagct gtgcgaactc
     1081 tttttcgtaa cgctccagct ccatccctaa cgcgcggtta aatggcatgt gataaacaaa
     1141 catctcaccc actaatttca gggcttgttc agcggtcagt acggcagaca tatcatcctt
     1201 acacttcatt ggttaatgaa atgttgattt tatgcttctt tgttgttggt ttctacttta
     1261 ggaagggata actaacggca atggagttaa gtatgtaaaa tagcccgcag aaaaacattc
     1321 accttatcaa taattcgtta cggagaacac gaccgatgcg gactctgcag ggctggttgt
     1381 tgccggtgtt tatgttgcct atggcagtat atgcacaaga ggcaacggtg aaagaggtgc
     1441 atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat gctgcaggag catgacaatc
     1501 cgttcacgct ctatccttat gacaccaact acctcatcta cacccaaacc agcgatctga
     1561 acaaagaagc gattgccagt tacgactggg cggaaaatgc gcgtaaggat gaagtaaagt
     1621 ttcagttgag cctggcattt ccactgtggc gtgggatttt aggcccgaac tcggtgttgg
     1681 gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa tagcgaagag tcttcaccgt
     1741 ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt tgccaccgat taccgttttg
     1801 caggttggac gctgcgcgat gtggagatgg ggtataacca cgactctaac gggcgttccg
     1861 acccgacctc ccgcagctgg aaccgccttt atactcgcct gatggcagaa aacggtaact
     1921 ggctggtaga agtgaagccg tggtatgtgg tgggtaatac tgacgataac ccggatatca
     1981 ccaaatatat gggttactac cagcttaaaa tcggctatca cctcggtgat gcggtgttga
     2041 gtgcgaaagg acagtacaac tggaataccg gttacggcgg cgcggagtta ggcttaagtt
     2101 acccgatcac caaacatgtg cgcctttata ctcaggttta cagcggctat ggcgaatcgc
     2161 tcatcgacta taacttcaac cagacccgtg tcggtgtagg agttatgcta aacgacttgt
     2221 tttgatgaac ggttgagtgg ttggcaaatc tggaacccag catccaggat taccctctca
     2281 gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa atagcgcctg tttttatttc
     2341 aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa tctggagtcc ggagctaaac
     2401 aggttttaca agaaaccttt ggctaccaac agtttcgccc cggccaggaa gaaattatcg
     2461 acactgtgct ttccggtcgc gattgcctgg tcgtcatgcc caccggtggc ggaaaatccc
     2521 tttgctatca aattcctgcc ttattgctaa acggccttac cgtggttgtt tcaccgctga
     2581 tttcgttgat gaaagatcag gtggatcaac tgcaagccaa cggcgtggcg gcggcgtgcc
     2641 ttaactcgac gcaaacccgc gagcagcaac ttgaagtgat gacaggctgc cgcaccgggc
     2701 aaattcgtct gctttatatc gccccggaac gcctgatgct ggataacttt cttgagcatc
     2761 tggcgcactg gaatccggtg ttattagccg ttgatgaagc gcactgtatc tcccaatggg
     2821 gccacgattt ccgcccggaa tatgccgcgc ttggtcagtt acgccagcgt ttcccgaccc
     2881 tgccgtttat ggcgctgact gccacagccg acgacaccac gcgccaggat atcgtgcgct
     2941 tgctggggct gaacgatccg ctgattcaaa tcagcagttt tgaccgtccg aatattcgct
     3001 acatgctgat ggagaagttc aaaccgctcg atcagttgat gcgctacgtg caggaacagc
     3061 gcggtaagtc aggcattatc tactgcaaca gccgcgcgaa agtagaagac accgctgcgc
     3121 gcctgcaaag caagggaatt agcgcggcgg cctatcatgc tgggctggaa aataatgttc
     3181 gcgccgacgt gcaggaaaaa tttcagcgcg atgacctgca aattgtggtg gcgacggtgg
     3241 cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt ggtccacttt gatattccgc
     3301 gcaatatcga atcctattat caggaaaccg gtcgcgccgg gcgtgatggc ctgcccgcgg
     3361 aagcgatgct gttttacgat ccggccgata tggcgtggct gcgccgttgc ctggaagaga
     3421 agccgcaggg gcagttacag gatatcgaac gtcataaact caatgcgatg ggcgcttttg
     3481 ccgaagcgca aacttgccgt cgtctggtat tgctgaacta ttttggcgaa gggcggcaag
     3541 agccgtgcgg gaactgcgat atctgcctcg atccgccgaa acagtacgac ggttcaaccg
     3601 atgctcagat tgccctttcc accattggtc gtgtgaatca gcggtttggg atgggttatg
     3661 tggtggaagt gattcgtggt gctaataacc agcgtatccg cgactatggt catgacaaac
     3721 tgaaagtcta tggcatgggc cgtgataaaa gccatgaaca ttgggtgagc gtgatccgcc
     3781 agctgattca cctcggcctg gtgacgcaaa atattgccca gcattctgcc ctacaactga
     3841 cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt gcaacttgcc gtgccgcgta
     3901 tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg cggcaactat gatcgcaaac
     3961 tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga tgaaagtaat gtcccgccgt
     4021 acgtggtgtt taacgacgca accttgattg agatggctga acagatgccg atcaccgcca
     4081 gcgaaatgct cagcgttaac ggcgttggga tgtgcaagct ggaacgcttt ggtaaaccgt
     4141 ttatggcgct gattcgtgcg catgtcgatg gcgacgacga agagtagtca gcagcataaa
     4201 aaagtgccag gatgaagact ccgtaaacgt ttcccccgcg agtcaaatgt atgttgatgt
     4261 tatttctcac cgtcgccatg gtgcacattg tggcgcttat gagccccggt cccgatttct
     4321 tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga agcgatgatg ggcgtgctgg
     4381 gtattacctg cggcgttatg gtttgggcag ggattgcgtt gcttggcctg catttgatta
     4441 tcgaaaaaat ggcctggctg catacgctga ttatggttgg cggtggcctg tatctctgct
     4501 ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga ggctgtatct gcgcctgcac
     4561 cacagattga gctggcgaaa agcgggcgca gtttcctgaa aggtttactg accaatctcg
     4621 ctaatccgaa agcgattatc tactttggct cggtgttctc attgtttgtc ggtgataacg
     4681 ttggcactac cgcgcgctgg ggcatttttg cgctgatcat tgtcgaaaca ctggcgtggt
     4741 ttaccgtcgt tgccagcctg tttgccctgc cgcaaatgcg ccgtggttat caacgtctgg
     4801 cgaagtggat tgatggtttt gccggggcgt tatttgccgg atttggcatt catttgatta
     4861 tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga taaggcgttt acgccgcatc
     4921 cgacattatt tttcacgcat gcctcgccga tgctaacagc gctcccacca gcataaacaa
     4981 cgagccgaaa atcttattca gcgccttcat ctgctttggt cctttaatcc atagagcaat
     5041 ccgttgagca agggtggcgt aaccgatcat cacaataata tcgactacaa tagtggtgac
     5101 gccgagcacg atatactgca tcagttgcgg ctgctgtggc atgatgaatt gcggaaatag
     5161 cgccgccaga aacacaatac ttttgggatt ggtgagattc acaaaaactg cgcgctggaa
     5221 caaatgtcga cgcgattgag tagaggcaag cgatttaagg tcaattgcac cagcggcgcg
     5281 ccactgctgg attcccagcc aaatcaagta agccgcgcct gcccacttca acacttcaaa
     5341 cgcaatcact gagcgggaaa atagcgtccc caaacccacg ccaaccagca caatatgaat
     5401 cgccagtccg gtctgaagcc cagcaataga cgccaccgcg ccgcgataac cgtggttgag
     5461 cgaggtggtc atagtgttga ttgcaccaga gcctggcgac aggcttaaaa tgatcgatgt
     5521 cagcaggtag gcaaaccacc attctaaggt catgatgaac tcccggtgtg tctatttttg
     5581 tgccacaata cgctactgtc gcagcgttgt gtcaggcacg ctaaaaaaaa cgattttacg
     5641 tggtttaaga ggcagattac ccgatgtttc agcagcaaaa agactgggaa acaagagaaa
     5701 acgcgtttgc tgcttttacc atgggaccgc tgactgattt ctggcgtcag cgtgatgaag
     5761 cagagtttac tggtgtggat gacattccgg tgcgctttgt ccgttttcgc gcacagcacc
     5821 atgaccgggt ggtagtcatc tgcccggggc gtattgagag ctacgtaaaa tatgcggaac
     5881 tggcctatga cctgttccat ttggggtttg atgtcttaat catcgaccat cgcgggcagg
     5941 gacgttccgg tcgcctgtta gccgatccgc atctcgggca tgttaatcgc tttaatgatt
     6001 atgttgatga tctggcggca ttctggcagc aggaggtcca gcccggtccg tggcgtaaac
     6061 gctatatact ggcacattcg atgggcggtg cgatctccac attatttctg caacgccatc
     6121 caggtgtatg tgacgccatt gcgctaactg cgccaatgtt tgggatcgtg attcgtatgc
     6181 cgtcatttat ggcacggcag atcctcaact gggccgaagc gcatccacgt ttccgtgatg
     6241 gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt tgctatcaac gtactgaccc
     6301 acagcagaca gcgatatcga cgtaacttac gcttctatgc tgatgaccca acgattcgcg
     6361 tcggtgggcc gacctaccat tgggtacgcg aaagtattct ggctggcgaa caggtgttag
     6421 ccggtgcggg tgatgacgcc acgccaacgc ttctcttgca ggctgaagag gaacgcgtgg
     6481 tggataaccg catgcatgac cgtttttgtg aactccgcac cgccgcgggc catcctgtcg
     6541 aaggaggacg gccgttggta attaaaggtg cttaccatga gatccttttt gaaaaggacg
     6601 caatgcgctc agtcgcgctc cacgccatcg ttgatttttt caacaggcat aactcaccca
     6661 gcggaaaccg ctctacagag gtttaaattt cttatgtacc aggttgttgc gtctgattta
     6721 gatggcacgt tactttctcc cgaccatacg ttatcccctt acgccaaaga aactctgaag
     6781 ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg gtcgtcacca cgttgatgtg
     6841 gggcaaattc gcgataatct ggagattaag tcttacatga ttacctccaa tggtgcgcgc
     6901 gttcacgatc tggatggtaa tctgattttt gctcataacc tggatcgcga cattgccagc
     6961 gatctgtttg gcgtagtcaa cgacaatccg gacatcatta ctaacgttta tcgcgacgac
     7021 gaatggttta tgaatcgcca tcgcccggaa gagatgcgct tttttaaaga agcggtgttc
     7081 aaatatgcgc tgtatgagcc tggattactg gagccggaag gcgtcagcaa agtgttcttc
     7141 acctgcgatt cccatgaaca actgctgccg ctggagcagg cgattaacgc tcgttggggc
     7201 gatcgcgtca acgtcagttt ctctacctta acctgtctgg aagtgatggc gggcggcgtt
     7261 tcaaaaggcc atgcgctgga agcggtggcg aagaaactgg gctacagcct gaaggattgt
     7321 attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt cgatggcggg gaaaggctgc
     7381 attatgggca gtgcgcacca gcgtctgaaa gaccttcatc ccgagctgga agtgattggt
     7441 actaatgccg acgacgcggt gccgcattat ctgcgtaaac tctatttatc gtaatcgttc
     7501 tttatttggt cagttgtcaa cctgatactt cgctacaatg gatactcgtt aatcaaagag
     7561 ttttccattg tggcgctact tatcatcacc acgattctgt gggccttctc ctttagcttt
     7621 tatggcgagt accttgcggg gcacgtcgat agctattttg cggtgctggt gcgcgttggc
     7681 ctggcggcac tcgtttttct gccgtttctg cgtacccgtg gcaatagcct gaaaacagtc
     7741 ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga tgtatatgct gagtttccgc
     7801 gcttatctct atctgacggt ttccgaactg ctgctgttca ccgtactgac gccgctctac
     7861 atcacgctga tttatgacat catgaataag cgccgtctgc gctggggata tgcttttagt
     7921 gccttgttgg cggtgattgg tgccgggatt attcgctatg atcaggtcac cgaccatttc
     7981 tggactggct tgttgctggt gcaactctcc aatatcactt ttgccatcgg catggtgggt
     8041 tacaaacgcc tgatggaaac tcgcccgatg ccgcagcata acgcctttgc gtggttctat
     8101 cttggcgcgt tcctggtggc ggtaattgca tggttcttgc tgggaaatgc gcagaaaatg
     8161 ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg gcgtggtggc ttccgggatt
     8221 ggctacttta tgtggaacta cggcgcgacg caggtggacg ccggaacgct gggaattatg
     8281 aataatatgc acgttccggc agggctgctg gtaaacctgg ctatctggca ccaacagccg
     8341 cactggccaa cgttgattac aggcgcgctg gtgatcctgg cctcactgtg ggtgcatcgt
     8401 aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc gcaggcgtga ttgcgcgctg
     8461 agcgaataaa cgcttccgta actggctgac gctgctcgcc atcgcgcacg gcggcgtaca
     8521 gtcggctcca caagccttcg cccagggttt tggtcaccac cagaccctgg cgctcaaaac
     8581 tctctactac ccaatgcggt agcgcggcaa tacccatccg cgcggcaacc atctgaatca
     8641 acaataaggt gttatcgacg cttttcagtg acggactgac gcctgccggc tgaagaaaat
     8701 gccgccagac atccagtcga ctacgctgca ccggataaat taatagcgtc tcgctggcga
     8761 gatcttccgg tgtaatacgc gtttttgccg ccagtggatg gtcaggtgct aacaccagac
     8821 gcacttcata gtcgaacatc ggcgaataat gcagaccact gcgcggcaga atatcggacg
     8881 tcattaccag atccagctct ccctgttgca aggcgggctg cgggtcaaat gtcacgcccg
     8941 atttaaagtc catctcgacc tgcggccagt tcttatggaa attttctaac gcgggcgtca
     9001 gccactgtat acagctatgg cactcaatgg caatgcgcag acgcgtctgc tgcggttcat
     9061 tgcaggcttg cagtgcctgg ctaatttgtg gcagcacctg gtttgccagt tgcaacagga
     9121 tttctccctg cggtgtaaag cgaagcggct ggcttttacg cacaaatagc cggaagccaa
     9181 ggcgttgttc cagatcgcta aactggtgag acagggcgga ttgcgtttga tgcaacgtcg
     9241 ccgcagcggc tgcgagtgag ccgcagttcc gcaacgcttg tagcgttttc agatgtttta
     9301 cttcgatcat gaaagtcctt cacttcggca tgaataattt gcgcttgagg aatatacagt
     9361 aaccgccaat tatggatgtg taaacatctg gacggctaaa atccttcgtc ttttaaattt
     9421 atggtgcgtt ggctgcgttt ctccaccccg gtcacttact tcagtaagct cccggggatg
     9481 aataaacttg ccgccttccc taaattcaaa atccatagga tttacatata attagaggaa
     9541 gaaaaatgac aatattgaat cacaccctcg gtttccctcg cgttggcctg cgtcgcgagc
     9601 tgaaaaaagc gcaagagagt tattgggcag ggaactccac gcgtgaagaa ctgctggcgg
     9661 tagggcgtga attgcgtgct cgtcactggg atcaacaaaa gcaagcgggt atcgacctgc
     9721 tgccggtggg cgattttgcc tggtacgatc atgtactgac caccagtctg ctgctgggta
     9781 acgttccggc gcgtcatcag aacaaagatg gttcggtaga tattgacacc ctgttccgta
     9841 ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc agcggaaatg accaaatggt
     9901 ttaacaccaa ctatcactac atggtgccgg aattcgttaa aggccaacag ttcaaactga
     9961 cctggacgca gctgctggag gaagtggacg aggcgctggc gctgggccac aacgttaagc
    10021 ctgtgctgct ggggccggtt acctggctgt ggctggggaa agtgaaaggt gaacagtttg
    10081 accgcctgag cctgctgaac gacattctgc cggtttatca gcaagtgctg gcagaactgg
    10141 agaaacgcgg catcgagtgg gtacagattg atgaacccgc gctggtactg gaactaccac
    10201 aggcgtggct ggacgcatac aaacccgctt acgacgcgct ccagggacag gtgaaactgc
    10261 tgctgactac ctattttgaa ggcgttacgc caaatctcga cacgattact gcgctgcctg
    10321 ttcagggact gcatgtggac cttgtacatg gtaaagatgg cgttgctgaa ctgcacaagc
    10381 gcctgccttc tgactggctg ctgtctgcgg gactgatcaa tggtcgtaac gtctggcgcg
    10441 ccgatcttac tgagaaatat gcgcaaatta aggacattgt cggcaaacgt gatttgtggg
    10501 tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct gagcgtggaa acgcgtcttg
    10561 atgcagaagt gaaaagctgg tttgccttcg ccttacaaaa atgccatgaa ctggcactgc
    10621 tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc agagtggagc gccccaattc
    10681 aggcgcgtcg tcactctact cgtgtacata atccggcggt agaaaagcgt ctggcggcga
    10741 tcaccgccca ggacagccag cgtgcgaatg tctatgaagt gcgtgctgaa gcccagcgtg
    10801 cgcgttttaa actgccagcg tggccgacca ccacgattgg ttccttcccg caaaccacgg
    10861 aaattcgtac cctgcgtctg gatttcaaaa agggcaatct cgacgccaac aactaccgca
    10921 cgggcattgc ggaacatatc aagcaggcca ttgttgagca ggaacgtttg ggactggatg
    10981 tgctggtaca tggcgaggcc gagcgtaatg acatggtgga atactttggc gagcacctcg
    11041 acggatttgt ctttacgcaa aacggttggg tacagagcta cggttcccgc tgcgtgaagc
    11101 caccgattgt tattggtgac gttagtcgcc cggcaccgat taccgtggag tgggcgaagt
    11161 atgcgcaatc tctgactgat aaaccggtga aagggatgct gactggcccg gtgaccattc
    11221 tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac catcgccaaa caaattgcgc
    11281 tggcgctgcg tgatgaagtg gcggacctgg aagccgcagg aattggcatc atccagattg
    11341 atgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag cgactgggat gcatatctcc
    11401 agtggggcgt ggaggccttc cgtatcaacg ccgccgtggc gaaagatgac acacaaatcc
    11461 acactcacat gtgttattgc gagttcaacg acatcatgga ttccattgcg gcactggatg
    11521 cagacgtcat caccatcgaa acctcgcgtt ccgacatgga gttgctggag tcgtttgaag
    11581 agtttgatta tccgaatgaa atcggtccag gcgtctatga cattcactcg ccaaacgtac
    11641 cgagcgtaga atggattgaa gccttgctga agaaagcggc aaaacgcatt ccggcagagc
    11701 gcctgtgggt caacccggac tgtggcctga aaacgcgcgg ctggccagaa acccgcgcgg
    11761 cactggcgaa catggtgcag gcggcgcaga atttgcgtcg gggatgatga atattaacga
    11821 gtcactgtaa attgcagtac gatgaaagag gcaaaactgc ctgataccag gcctacataa
    11881 tttatgcaat ttattgaatt tttgagtttt gttggccgga caaggcgttc acgccgcatc
    11941 cggcatgaac aacgcgcact ttgccaataa tctgatggct tccatcaccg gaagccattt
    12001 tgctatctgc gcccgttcag atacagaaat aaccgaatca tttttacgca aaatataatc
    12061 ctgagcgatt tgttcagtaa tcaacgctac gttatttatt ctcacttttc acgaaattgt
    12121 atcccttttc agtaacgtgg tagtgaggca tagacttatg tccgctatcc tcaataataa
    12181 ccaaaccctc gttagctaat ttgttaacaa tgttaacaac attaccgaat cctggaattt
    12241 gccttatgga tagtgtgcga tcgagattca tccagttcca gtttatatct ttctcagcca
    12301 taactttcag tacgttaatt tcagtttcag ttatcatagt caagtgcatc ctggtggtat
    12361 aagtcattaa gttctccctt aagtttataa tcttctagag tagcagtatg cgtatgattc
    12421 cagagtgcat agcctgcgtc accatcaggt tgtcctgttt cccaaccaag attacagtaa
    12481 cgctgatatt cccgacactc atgagtatag aagttaagat ctacttgttc tggggggatt
    12541 tttcctttct caatgtcttt cagtctattg atcatcactt catttacagg gtcatgacca
    12601 aatctggcta tgtgtttttc aacaatagcg ataccttccc tgttgattgt tacgccatct
    12661 gtcgtcagat tttctattgg accaccggca aggtcgggat taaatgg
//
LOCUS       DAJTNN010000119        12660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTNN010000119 DAJTNN010000000
VERSION     DAJTNN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.119"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO150_002906"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO150_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5516983.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OO150_002907"
     CDS             1946..2230
                     /locus_tag="OO150_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5516984.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3302
                     /locus_tag="OO150_002908"
                     /pseudo
     CDS             2410..3302
                     /locus_tag="OO150_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3302..5446
                     /gene="fadJ"
                     /locus_tag="OO150_002909"
     CDS             3302..5446
                     /gene="fadJ"
                     /locus_tag="OO150_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5516985.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTVQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTTLEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            5649..6134
                     /gene="sixA"
                     /locus_tag="OO150_002910"
     CDS             5649..6134
                     /gene="sixA"
                     /locus_tag="OO150_002910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5516986.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            6809..7069
                     /locus_tag="OO150_002911"
     CDS             6809..7069
                     /locus_tag="OO150_002911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5516987.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            7076..7372
                     /locus_tag="OO150_002912"
     CDS             7076..7372
                     /locus_tag="OO150_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5516988.1"
                     /translation="MTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSY
                     KSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7454..10096
                     /gene="yfcU"
                     /locus_tag="OO150_002913"
     CDS             7454..10096
                     /gene="yfcU"
                     /locus_tag="OO150_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR5516989.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQIRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQINYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            10116..10868
                     /locus_tag="OO150_002914"
     CDS             10116..10868
                     /locus_tag="OO150_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5516990.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            10884..11393
                     /locus_tag="OO150_002915"
     CDS             10884..11393
                     /locus_tag="OO150_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5516991.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            11390..11878
                     /locus_tag="OO150_002916"
     CDS             11390..11878
                     /locus_tag="OO150_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5516992.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            11875..12414
                     /locus_tag="OO150_002917"
                     /pseudo
     CDS             11875..12414
                     /locus_tag="OO150_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         3202 a         3024 c         3333 g         3101 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttcgtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgttgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgttgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgttgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatggttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgcgcgat ttaatgcccg taccgcctgc agtagcagaa
     2941 tattctaccg gcttgcggat gggtgacacc gcagagcaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgcgtg gatgattacc cagacattgc atgaacttcg ccgtcgcggc ggtggatttg
     3241 gtttagttac cgcctgtgct gccggtgggc ttggcgcggc aatggttctg gaggcggaat
     3301 aatggaaatg gcatcagcgt ttactcttaa tgttcgtctg gacaacattg ctatcatcac
     3361 catcgacgta ccggatgaga aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg
     3421 cgccattatt aagcaactcc gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc
     3481 gaaaccggac aattttatcg ccggagcaga catcaacatg atcggcaact gcaaaacggt
     3541 gcaagaagcg gaagctctgg cgcggcaggg ccaacagttg atggcggaga ttcatgcttt
     3601 gcccattccg gttatcgcgg ctattcatgg cgcttgcctg ggtggtgggc tggagttggc
     3661 gctggcgtgc cacggtcgcg tttgtactga cgatcctaaa acggtgctcg gtttgcctga
     3721 agtacaactt ggattgttac ccggttcagg cggcacccag cgtttaccgc gtctgatagg
     3781 cgtcagcaca acattagaga tgatcctcac cggaaaacaa cttcgggcga aacaggcatt
     3841 aaagctgggg ctggtggatg acgttgttcc gcactccatt ttgctggaag tcgctgttga
     3901 gctggcgaag aaggatcgcc catcttcccg ccctctacct gtacgcgagc gtattctggc
     3961 ggggccgtta ggtcgtgcgc tgctgttcaa aatggtcggc aagaaaacag aacacaaaac
     4021 tcaaggcaat tatccggcga cagaacgcat cctggaggtt gttgaaacgg gattagcgca
     4081 gggcaccagc agcggttatg acgccgaagc tcgggcgttt ggcgaactgg cgatgacgcc
     4141 acaatcgcag gcgctgcgta gtatcttttt tgccagtacg gacgtgaaga aagatcccgg
     4201 tagtgatgca ccgcctgcgc cattaaacag cgtgggaatt ttaggtggtg gcttgatggg
     4261 cggcggtatt gcttatgtca ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat
     4321 caacccgcag ggcataaatc atgcgctgaa gtacagttgg gatcagctgg agggcaaagt
     4381 tcgccgtcgt catctcaaag ccagcgaacg tgacaaacag ctggcattaa tctccggaac
     4441 gacggactat cgcggctttg cccatcgcga tctgattatt gaagcggtgt ttgaaaatct
     4501 cgaattgaaa caacagatgg tggcggaagt tgagcaaaat tgcgccgctc ataccatctt
     4561 tgcttcgaat acgtcatctt taccgattgg tgatatcgcc gctcacgcca cgcgacctga
     4621 gcaagttatc ggcctgcatt tcttcagtcc ggtggaaaaa atgccgctgg tggagattat
     4681 tcctcatgcg ggtacatcag cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca
     4741 gggcaaaacg cccattgtcg tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc
     4801 gccttacatt aatgaagcta tccgcatgct gaccaaaggt gaacgggtag agcacattga
     4861 tgccgcgcta gtgaaatttg gttttccggt aggtccaatc caacttttgg atgaggtagg
     4921 aatcgacacc gggactaaaa ttattcctgt actggaagcc gcttatggag aacgttttag
     4981 cgcgcctgca aatgttgttt cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg
     5041 tcggggtttc tatctttatg gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcccgc
     5101 catttacccg ctcattggcg cacaagggca ggggcgactc tccgcaccgc aggttgctga
     5161 acggtgtgtg atgttgatgc tgaatgaagc agtacgttgt gttgatgagc aggttatccg
     5221 tagcgtgcgt gacggggata ttggtgcggt atttggcatt ggttttccgc catttctcgg
     5281 tggaccgttc cgctatatcg attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg
     5341 actggccacg cagtatggtt cccgttttac cccttgcgag cgtttggtcg agatgggcgc
     5401 gcgtggggaa agtttttgga aaacaactgc aactgacctg caataagaag gtcaaagcta
     5461 tatgaatccg cgctgaatgg cggagtgttg gtcaaaatgt aaacgcatat tgactatact
     5521 tacgccattg aggtaaaaaa cagcgtttca ttcggtgaat ggataaggca caatgccggc
     5581 caccgttttc tttctctggt ttcagatgaa agaaaatggg cgaatctggt taacaaaagc
     5641 ggtgcaatat gcaagttttt atcatgcgtc acggcgacgc agccctcgat gccgccagtg
     5701 attcggttcg tcctctgacc actaatggtt gtgacgaatc tcgcctgatg gcgaactggc
     5761 tgaaaggtca aaaagtggaa atcgaacgcg ttctggtgag cccgttcctg cgagccgagc
     5821 aaacactgga agaagtaggg gattgtctga acctgccttc cagtgcagaa gttctgccgg
     5881 agttaacgcc ctgcggcgat gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg
     5941 aaggtgttgc ttcggtactg gtgatctctc acttaccgtt agtcgggtat ctggttgccg
     6001 agttatgtcc gggcgaaacg ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc
     6061 ttgatgaaag cggaaacggt acattcaact ggcagatgag cccgtgtaat ctgaagatgg
     6121 caaaagctat ctgatgtgtt ccaaattatg cccaatgagg tagattcatt gggcataatt
     6181 gctcatcagg cagcggatat ctgtcaacct cactactatc gaagactctc cttatctata
     6241 aataaaacac tttctacatt cattcggaaa ttattcttct gcacgtatct tgattttgcc
     6301 caaagttaca tgttgaaatg aataatgtct gtttgacact cattgtcatt tattaataca
     6361 gataatagag ttgaataatc aggcaggatg taattgaaac ttgattaaat tctggctata
     6421 gtgtttatat attatattgt caaaagtgga taaatgccaa tcgcattata tattcattgt
     6481 ttgtttttta agctgttgat attcattatt gatatacttt ctattttttc taaatactta
     6541 ttccgtattt tttaaggatg tccttaagat aatctttctt ctgtcaatat attaattaaa
     6601 tctacattta ctctttagta aaattaaatt gcatcagggg tgtggaaaca tcactatgcg
     6661 gccccacacc aacgaataca attttctacg acctgttctt tttggatcgc atgccgtatt
     6721 caatggctgg gtataaacat tggctattga ctgaatcaat gtattgcaat gtttatattt
     6781 ttaattattt atagtttaag gacaaataat gagtaagttt gttaaaacag ctattgctgc
     6841 ggcaatggtg atgggcgtgt tcacctctac ggcaacaatc gctgcgggta acaacggtac
     6901 tgcacgtttt tacggcacca ttgaagactc agtgtgttct atcgttccgg acgatcacaa
     6961 actggaagtt gatatgggtg atatcggtgc cgaaaaactg aaaaataacg gcaccaccac
     7021 gccgaaaagt ttccagattc gtctgcaaga ttgcgtattt gatacttagg aaacaatgac
     7081 cactaccttt actggtaccg tttcttctgc aaatagcggc aattattaca ccattttcaa
     7141 taccgatacc ggtgcggcat ttaacaatgt tagcctggcg atcggtgact ctctgggtac
     7201 ctcttacaaa agcggcatgg gtattgacca gaaaatagtg aaagacactt ctactaataa
     7261 aggcaaagcg aagcagacac tgaactttaa agcctggctg gtcggcgcag ctgatgcgcc
     7321 agatctgggt aatttcgaag caaataccac cttccagatt acttacctgt aaggcatacc
     7381 cccatagcga cactgctatg ggggatttaa atggattttg accgaacaag aataaaaacg
     7441 gatagaaacg tgtatgcctg accattctct ttttcgatta cggattcttc cgtggtgcat
     7501 tgcgctggca atgtcaggga gttatagcag tgtctgggct gaagacgaca ttcagtttga
     7561 ttcccgtttt ctggaattaa aaggcgacac aaaaattgat ctgaagcgtt tttccagcca
     7621 gggatatgtt gagcccggaa aatacaattt acaggttcaa ctaaataaac agccattggc
     7681 ggaagagtac gatatttact ggtatgctgg tgaagatgac gcgagcaaaa gctatgcttg
     7741 tctgacaccg gaactggtag cgcagtttgg tttaaaagaa gacgtggcga aaaatctgca
     7801 atggagccac gatgctaaat gcctgaaatc cggtcaactg gaaggcatgg aaattaaggc
     7861 tgatttaagc cagtccgcat tagtcatttc actgccacag gcttacctcg aatatactta
     7921 tcccgactgg gatccgcctt cacgttggga tgacggcatc tccgggatcg tcgcggacta
     7981 cagcatcaac gcacaaaccc ggcacgaaga aaatggcggt gatgatagta acgagatcag
     8041 cggcaacggg acggtcgggg ttaacctggg gccgtggcgt atgcgtgctg actggcagac
     8101 taactatcaa catactcgca gtaatgatga cgatgaattc agcggcgatg aaactcaaaa
     8161 aaaatgggag tggagtcgct actatgcctg gcgggcgtta ccatcattaa aagccaaact
     8221 ggcgctgggc gaggattacc tcagatccga tatttttgat ggttttaact atgttggtgg
     8281 tagtgtcagt actgacgatc aaatgttgcc tcccaatctg cgcggctacg cgccagacat
     8341 ttccggcgtg gcacacacca cagcaaaagt gaccgtcagc cagatggggc gtgtgattta
     8401 cgaaacgcag gttccggctg gaccgtttcg tattcaggat cttggtgatt ctgtctccgg
     8461 tacgttgcat attcgcattg aagaacagaa cggccaggtg caggaatatg acatcagcac
     8521 cgcctcgatg ccatacctca ctcgtccagg tcagattcgc tataagatca tgatgggccg
     8581 tccgcaagag tggggacacc atgtcgaggg tgaatttttt tctggtgctg aagcttcctg
     8641 ggggatcgct aacggctggt cgttatatgg cggcgcactg ggagatgaaa actatcagtc
     8701 tgcggcgctt ggcgtcggtc gcgatttgtc tacattcggc gcggtcgcgt ttgatgttac
     8761 tcactcgcac accaaactgg ataaagacac cgcttatggc aaaggttcgc tggacggtaa
     8821 ctccttccgt gtgagttatt ccaaagactt tgaccagctc aacagccgcg ttacttttgc
     8881 tggatatcgc ttctcggaag agaactttat gaccatgagc gagtatctgg atgccagtga
     8941 cagcggaatg gtacgcacgg gcaacgacaa agagatgtac accgccactt ataaccagaa
     9001 cttccgcgat gcgggtgttt cggtttatct caactatacc cgccatacct actgggatcg
     9061 cgaggagcag ataaactaca acatcatgct ctcgcactat ttcaatatgg gcagtattcg
     9121 taatgtcagc atctcgatga ctggctaccg ttacgagtat gacaaccagg ccgacaaagg
     9181 catgtacatt tcgctcagta tgccgtgggg cgacaacagt accgttagct ataacggtaa
     9241 ctatggcagt gggacggaca gcagtcaggt cggttatttc agccgtgtcg atgacgcgac
     9301 tcactatcag ttgaacgtcg gcaccagtga caaacacacc agcgttgacg gctattacag
     9361 ccatgatggt tcgctggcgc aggttgacct cagtgcgaac taccatgaag ggcaatacac
     9421 ctctgcgggc ttgtcgttac agggcggcgc aacgcttact gcccacggtg gcgcacttca
     9481 ccgtacccag aatatgggcg ggacacgctt gttgattgat gccgatggcg ttgccgatgt
     9541 tccggtggaa ggtaacgggg ctgctgttta taccaatatg tttggtaaag ccgtcgtttc
     9601 tgacgtcaat aactattacc gcaatcaggc gtatatcgac ctcaacagat tgcctgaaaa
     9661 cgctgaagca acccagtcgg tggtgcaagc cacgctaact gaaggtgcca ttggctaccg
     9721 taaatttgct gtcattagcg gacaaaaagc gatggcggtg ctgcgcctga gcgacggcag
     9781 ccatcctccg tttggcgcag aagtaaaaaa tgataacgag cagacagtgg gccttgtcga
     9841 tgatgacggc aatgtttatc tggctggggt gaaacctggc gaacacatga gtgtgttctg
     9901 gagtggtgtt gcgcattgcg atatcaacct gccggacccg ctgcctgccg atctgtttaa
     9961 cggcttgtta ctgccatgcc agcataaagg caatgtagca cctatcactt cgccggcggt
    10021 caaaccggcg attcaggaac agacacagcg ggtgacgcca acggaacctc cgacttcaat
    10081 ttcagtaaac cagtaacgtg attaaggaat gatccatgtt taatctgact aacactgcaa
    10141 aaatcgttgt cccggcactg gcgctgctgg cgacagcggt cagtttctcc agccacgctt
    10201 cggtgacgcc cgacaggaca cggctggtgt ttaacgaaag cgacaagtcc atcagcgtca
    10261 cgctgcgcaa caacgatccc acactgcctt atctggcgca gagctggata gaggatgcaa
    10321 agggcaacaa aatcagctct ccgcttgcgg tgcttccgcc agtgcagcgc atcgatgcga
    10381 tgatgaacgg ccaggtgaag gtgcaggggc tgccggatat caacaaactg ccggccgacc
    10441 gcgagagcgt gttctatttc aacgtgcgcg aaattccgcc gaaatcaaat aaaccgaaca
    10501 cactgcagat tgcgctgcag acgcgtatca aactgttctg gcgaccgaag gcgctggaga
    10561 acaccagcat gaaaagtccg tggcagcata aggtgacgct catccgtaac ggccaggact
    10621 tcaccgtcaa taacccgacg ccgtactacg tgatcatcag caacgccagc gctaaaaaag
    10681 gcggcaaccc gccggcggga ttcacaccgc tgatgatgga gccgaaaacc tccgcaccgc
    10741 tgaatgcgaa gatggacagc gtgccggtgc tgacttacat caacgactac ggcgcgcgga
    10801 tgccgctgta cttcaaatgc accggcaaca gctgccaggt ggatgaagag cagagccgca
    10861 aaggctgatg ccgggaggga cttatgagtc acaagtttgt agcgggcgca gggttgctgg
    10921 tgctggccgg atggggcatc agcggtttgg ctgttgctgg cagtgatatc aaacaggtgg
    10981 atctgacgct gcgaatccta gtcgatggac cgccgccttg ctccgtgaag ggaagcgctg
    11041 tgaaatttgg caatgtgata atcaacagga tcgatggtac caattaccaa caggatgcga
    11101 aatacaccct caactgcggt aataaagtca gcgatgactt acggatgcag ctcaaaggca
    11161 ataccgccgt gatcaacgga gaaacggttc tcagcacgga tatcgccggc ctgggggtgc
    11221 ggatagaaaa cgccgccgac aactccctgt ttgcagttgg tgaaaataac tggacgcctt
    11281 ttaatgtcaa cactcagcct cagctgaaag cggtgccggt aaaacagagc ggggtgcaac
    11341 tgactccgtc ggagtttaac gccacgatga ccatggtggt ggattaccaa tgaaaaaatt
    11401 cctgctcctt ttactgtgcg ctggttgcgg ggctgtgctg gcggcggaag atgacatcac
    11461 attccacggt acgctgatgt cgccgcccgc ctgtagcatc agcggcgggc aaaccatcga
    11521 agtgcaattt cgcgatctca ttatcgacag cattgatggc aattatggtc gtcagaaagt
    11581 ggactacgaa ctgagctgcg actcggatgt ccgcgatccc gagtggaata tgacactctc
    11641 ctggacgggg actgccacct cctacaacga ctctgctatt gagacggatg taccgggctt
    11701 cggaattgag cttcagcatg acggtcagcg ttttaagctc aacgagccgc tttccattaa
    11761 cgccaccgat tttagccaga agccaaagct ggaggcggta ccggtcaaag cggctgatgc
    11821 ggtgctgacg gacacgacct tttctgctta cgcaacctta cgggtggatt accaatgaaa
    11881 acagcacaca tttattcacc cggttatgcg ctgttagcag gcctgctgct gaccgccagc
    11941 acaggagcac aggcagtcga caacaacctg catttttacg gcaacctgct gagtaaatcc
    12001 tgcacgctgg tggtggatgg tgccaacctg gcagaaatcc attttcccac cgtcagcaac
    12061 cgggatttga tggtatctgg gcagtccgcg catctgccgg ttgtgtttaa gctgaaagac
    12121 tgcaaagggc cggcgggata caacgtcaaa gtcacgctga gcggcaccga agacagcggt
    12181 cagccgggtt ttctggcagt cgatgacacc tccaccgcgc agggcgtggg gatagggatg
    12241 gaaacgctcg acggcaagcg ggtggatatt aacaacacca ccggggcgac ctttgcgctt
    12301 accaacggca acaatgatat taatttccgg gcctggatct aggcgaaaag cggtcgtgat
    12361 gtaaccatcg gtgatttcac cgccagcctg acagcaactt ttgagtatat ttaaaatgaa
    12421 aatccttcgt catgttttat gtcttttatt gctgttaatg ttcaatacat gcggccaggc
    12481 ggcgacgaaa gttggtgagt tatatgtgcc agctggtgcc agttcacctg cgacagagtg
    12541 gatttgcccc tatatttcca gacatctgtt atcacttaac ccattacaag cccgctgccg
    12601 cagatattcc cgtggcgagc gataacccag cgcactatgc ggatgccatt cgttataatg
//
LOCUS       DAJTNN010000120        12573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTNN010000120 DAJTNN010000000
VERSION     DAJTNN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.120"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            224..1321
                     /gene="mltA"
                     /locus_tag="OO150_002918"
     CDS             224..1321
                     /gene="mltA"
                     /locus_tag="OO150_002918"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5516993.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1398..2204
                     /gene="tcdA"
                     /locus_tag="OO150_002919"
     CDS             1398..2204
                     /gene="tcdA"
                     /locus_tag="OO150_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5516994.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2255..2698)
                     /gene="csdE"
                     /locus_tag="OO150_002920"
     CDS             complement(2255..2698)
                     /gene="csdE"
                     /locus_tag="OO150_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5516995.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVLGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2698..3903)
                     /gene="csdA"
                     /locus_tag="OO150_002921"
     CDS             complement(2698..3903)
                     /gene="csdA"
                     /locus_tag="OO150_002921"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5516996.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4095..4322
                     /locus_tag="OO150_002922"
     CDS             4095..4322
                     /locus_tag="OO150_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5516997.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4673..5590
                     /gene="gcvA"
                     /locus_tag="OO150_002923"
     CDS             4673..5590
                     /gene="gcvA"
                     /locus_tag="OO150_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5516998.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5609..6004
                     /locus_tag="OO150_002924"
     CDS             5609..6004
                     /locus_tag="OO150_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5516999.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5997..7097
                     /gene="rlmM"
                     /locus_tag="OO150_002925"
     CDS             5997..7097
                     /gene="rlmM"
                     /locus_tag="OO150_002925"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5517000.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENSGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7141..7872)
                     /gene="fucR"
                     /locus_tag="OO150_002926"
     CDS             complement(7141..7872)
                     /gene="fucR"
                     /locus_tag="OO150_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5517001.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICYELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7930..8352)
                     /gene="fucU"
                     /locus_tag="OO150_002927"
     CDS             complement(7930..8352)
                     /gene="fucU"
                     /locus_tag="OO150_002927"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5517002.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8354..9772)
                     /gene="fucK"
                     /locus_tag="OO150_002928"
     CDS             complement(8354..9772)
                     /gene="fucK"
                     /locus_tag="OO150_002928"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR5517003.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERTHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILIVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9769..11514)
                     /gene="fucI"
                     /locus_tag="OO150_002929"
     CDS             complement(9769..11514)
                     /gene="fucI"
                     /locus_tag="OO150_002929"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5517004.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(11547..>12573)
                     /gene="fucP"
                     /locus_tag="OO150_002930"
     CDS             complement(11547..>12573)
                     /gene="fucP"
                     /locus_tag="OO150_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5517005.1"
                     /translation="LFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPF
                     VTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSA
                     YKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARI
                     RHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTG
                     TWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGI
                     KNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARF
                     RSQTATN"
BASE COUNT         3008 a         3147 c         3329 g         3089 t
ORIGIN      
        1 ctgctccacc ccgcgtccgt ggatgcgcac tatactccgc tcgcgttttc atgcaacctt
       61 ttttttaccc cgatcacgga tttagcatga ttttgaacaa aaacaaacca ctaaagccta
      121 ttttttgtgc aaaatttgcg tcagggagag gctgctcatt tcattaaacg cggcggtttg
      181 ttatcttcgt tgcgccttat tttttaacct gaagaagaga acaatgaaag gacgttgggt
      241 aaagtacctt cttatgggca cggttgtggc aatgcttgcc gcctgctctt ccaaatcaac
      301 cgatcgcgga cagcaatata aagacgggaa atttacccag cctttctctc tggtgaacca
      361 gccagatgcc gttggcgcgc cgattaacgc cggtgatttt gccgagcaaa ttaaccatat
      421 ccgtaattcg tcaccgcgtc tgtatggcaa ccagagtaat gtttataacg cggtgcaaga
      481 gtggctgcgc gcaggcggtg atacccgcaa tatgcgccag ttcggcattg atgcctggca
      541 gatggaaggt gccgacaact atggtaacgt gcagtttaca ggctattaca cgccggtaat
      601 tcaggcgcgc catacccgcc agggcgagtt ccagtatcct atttaccgta tgccgccaaa
      661 acgtggtcgt ctgccgtctc gtgcggagat ctacgcgggg gcattgggcg ataaatacat
      721 cctcgcttac agcaactctc tgatggataa cttcattatg gatgtgcagg gtagtgggta
      781 tatcgacttt ggtgatggca gtccgcttaa ctttttcagc tatgcaggga aaaacggtca
      841 tgcctatcgc agcattggta aggtgctgat cgaccgtggc gaagtgaaaa aagaagatat
      901 gtcgatgcag gcgattcgtc attggggcga aacacacagt gaagccgagg ttcgcgagct
      961 gctggaacag aacccgtctt tcgtcttctt taaaccgcaa tcttttgcac cggtgaaagg
     1021 ggcaagtgcg gtgccgctgg ttggccgtgc ttctgttgcc tccgatcgtt caattattcc
     1081 gccaggtact accttgctgg cagaagtgcc gctgctggat aataacggca aatttaacgg
     1141 tcagtacgaa ttgcgtctga tggtggcgct ggatgtcggt ggcgcaatca aaggccaaca
     1201 cttcgatatc tatcaaggga tcgggccgga agccggacat cgcgcaggtt ggtacaacca
     1261 ctatggacgt gtctgggtgc tgaaaaccgc cccgggcgca ggtaacgtct ttagcggctg
     1321 atgtgttatt ctgtataccg tttttacgga tacgacaggg tgagggataa cctcaccctt
     1381 tttaattctg aggtgctatg tctgtggtaa ttagtgatgc atggcgtcag cgttttggtg
     1441 gcacagcgcg tctgtatggt gaaaaagcgt tgcaactgtt tgctgacgcg catatttgtg
     1501 tggttggtat cggtggtgtc ggttcctggg cggcggaagc gctggcgcgc acggggattg
     1561 gcgcaatcac gcttatcgat atggatgatg tgtgcgtcac caataccaat cggcaaattc
     1621 acgctctgcg cgataacgtc gggctggcaa aagcggaagt catggcggag cgcattcgcc
     1681 agattaaccc ggagtgccgt gtaacggtgg tggatgattt cgtgacgccg gataacgtag
     1741 cgcaatatat gagtgccggt tattcgtacg tgattgatgc cattgatagt gtacgaccca
     1801 aagcggcgct gattgcttat tgtcggcgca ataaaatccc gctggtcacg accggtggcg
     1861 cgggtgggca gattgacccg acgcagattc aggttaccga tctggcgaaa acgattcagg
     1921 acccgctggc ggcgaagttg cgcgagcgcc tgaaaagcga ttttggcgta gtgaaaaaca
     1981 gtataggtaa gctcggcgtg gattgcgtgt tttctactga agcgctggtg tacccgcagt
     2041 cagacggtac ggtgtgtgcg atgaaagcca cggcagaagg gccgaagcgg atggattgtg
     2101 catctggatt tggcgcggca acgatggtga ccgccacctt tgggtttgtt gcggtttctc
     2161 atgcgctgaa gaagatgatg gcgaaagcag cgcgtcaggg ttaaacaatc aggtcggatg
     2221 cgacgcgaac gtcttatccg acctggttct gtgcttaaac ctgcttcgcc gcagcgataa
     2281 ttgcctcgct taacgcattc aacccctggc tgcgtgaggc gctaagctgc ccacgcaatc
     2341 ccagctcatc aaacaatgcc agcggcgact gtgcctgtaa ttcggcggcg gttttcccct
     2401 caacggcagt caacaacacc gccaacaggc cgagcacaat gcgcccttcg ctgtcgccaa
     2461 agaagtgcat tttaccgttt tcagccacgg tatatcccag ccagacgcgg ttttcgcatc
     2521 cggcaatctc tttagcctgc gcttttaact cgtctggcaa agccggaagc tgtttcccca
     2581 gcatgatcaa ctggcgatat ttatcttccc attgcgtcaa cggtgcgaag gtattgcgta
     2641 acgtttctgc ggttacggtt gtgccgaacg gatgtccggc gaattgcggg tttgtcatta
     2701 atccaccaat aattccagcg cgcggtcaac ggcattcacc agcgcatcca catcactctt
     2761 tgtattatat ggcgcaaaag aggcgcgcag tgtgccggtt acgcctaatt ctgccagtag
     2821 cggctgagca caatgttgcc cggcccgcaa ggcaataccg tactccgcca gtagcgtcac
     2881 catatcgctg tgatgaacgc cagcaaaatc aaaggccagc aggctggaat cctggcagcg
     2941 gaatgaacga aagcctggac gtttcgccag cgcatcttcc gccagcgttg ctaagctatg
     3001 gctccagttt tcggcctggt tgatatcgta atctgccagc cattccagcg ccgcgcttaa
     3061 tcctatgaca ccagccacat ttggcgttcc tgcttccagt ttccacggcg cagattgagt
     3121 cgtgaagccg tcaaaactca cttcgtgaat cattttgccg ccacccagcc agggcgacat
     3181 cgcttccagc agttctgatt tgccatacag cacgccgata cccgtcgggc catacagttt
     3241 gtgaccagag aaagcataga aatcgatatc cagttgctga acatccgcgg ggaaatgcac
     3301 cgccccctga gcaccatcaa ccatcaccac catccctgct gaatgagcaa aggtaatcgc
     3361 tcgcgccaga tccgggcaac cgccagtaac gttcgacatc tgactcaacg ccagaatccg
     3421 actacgggga gtaatcagtt ctggcaacaa atcgacatcc ggcagtcgct gcgcattaag
     3481 cggcaatttc accactttgg ctccagtttg ttgggcgacc atcagccagg ggacgaggtt
     3541 ggcgtggtgt tctgccacgc tgacaataat ctcatcgccc ggttgcagac gcggacgcgc
     3601 atagcattgt gccaccatgt tgatggattc agtggtgccg cgcgtccaga cgatagtttt
     3661 atcatccggt gcattcagta attgcgccac tttctctcgt gcagcttcat aacgcgcggt
     3721 caggcgttgg gcttcggcaa actggctgcg atggacgttt ccggcgctca ggctgtaaaa
     3781 ctgtcgagtg gcttcaacca cggcttcagg tttaagcgcg gtcgcggcgc tgtcgagata
     3841 gacgcccgca tcctgtagtg cgggaaactg ggcgcgaaac tgcgcgggat taaaaacgtt
     3901 catggtactc ctcggcttga tgcaccgatc gtgacgcaaa attcactttg atacaaggag
     3961 agtgctaacc atcagaatta tctggatatc ctggcgaagc cgcgtaagta acatatgctg
     4021 aatagtgtta ggcgttaatt ttagcctgtt aatcaatact attaagattt attaatcgct
     4081 ttaaggagaa gatgatgaaa aagactgccg caattatttc tgcctgtatg ctgacttttg
     4141 ccctgagcgc ctgttccggt tcgaactatg tgatgcacac caatgacgga cgtaccatcg
     4201 tctctgacgg caaaccacag actgataacg ataccggtat gatttcgtat aaagacgcta
     4261 atggcaacaa acagcagatc aaccgtactg acgtgaaaga gatggtcgaa ctggatcagt
     4321 aattcgcgat cgcaaggtaa aaaaaagcac cgcaattagg cggtgctaca ttaatcacta
     4381 tggacagaca gggtaaatgt acaggaagtg aaaaaaggta gctttgctac catggtctga
     4441 atcgcagacc aattgcaaac acaacaacac aacatcacaa ccgtaagcca aaagttcacc
     4501 agaacacgca ttccgataaa acttttcgtt ccggctcagg aagtgccgcc actataggta
     4561 tttgctggta gaagctcaac ggacaattta taatggctca gattaaaaaa actaataggt
     4621 tacatagtgt gatctaattg ttaaattcat ttaacatcaa agtttaatag ccatgtctaa
     4681 acgattacca ccgctaaatg ccttacgagt ttttgatgcc gcagcacgcc atttaagttt
     4741 cactcgcgca gcagaagagc tttttgtgac ccaggccgca gtaagtcatc aaatcaagtc
     4801 tcttgaggat tttttggggc taaaactgtt ccgccgccgt aatcgttcac tcctgctgac
     4861 cgaggaaggg caaagctatt tcctcgatat caaagagata ttttcgcaat taaccgaagc
     4921 gacgcgtaaa ctccaggccc gtagcgccaa gggggcgttg acggtcagtt tactccccag
     4981 tttcgccatt cattggttgg ttccgcgact ttccagcttt aattcagctt atccgggaat
     5041 tgacgttcga atccaggcgg ttgatcgtca ggaagataag ctggcggatg atgttgatgt
     5101 ggcgatattt tatggtcggg gcaactggcc ggggctacgg gtggaaaaac tgtacgccga
     5161 atatttattg ccggtgtgtt cgccgctact gctgactggc gaaaaaccct tgaagacacc
     5221 ggaagatctg gctaaacata cgttattaca tgatgcttcg cgccgtgact ggcagacata
     5281 tacccgacag ttggggttaa atcatatcaa cgttcagcaa gggccaattt ttagccatag
     5341 cgccatggtg ctgcaagcgg ctatccacgg gcagggagtg gcgctggcaa ataacgtgat
     5401 ggcgcaatct gaaatcgagg ccggacgtct tgtttgcccg tttaatgatg ttctggtcag
     5461 taaaaacgct ttttatctgg tttgtcatga cagccaggca gaactgggta aaatagccgc
     5521 ctttcgccaa tggatcctgg cgaaagccgc tgctgaacaa gaaaaattcc gctttcgtta
     5581 tgaacaataa tttacgtagg gtacgaccat gaccagccgt tttatgctga ttttcgccgc
     5641 cattagcggc ttcatttttg tggctctggg cgcttttggc gcgcatgtgt taagtaaaac
     5701 catgggagcc gttgagatgg gctggatcca gaccggcctc gaataccagg cgtttcatac
     5761 gctggcgatc ttaggtctgg cggtggcaat gcagcgtcgc atcagtatct ggttttactg
     5821 gagtagcgtt ttcctcgcgt taggcacggt gttgttcagc ggcagccttt attgcctggc
     5881 gctgtcccat ctgcgtttgt gggcgtttgt cactccggtt ggcggcgtga gcttcctcgc
     5941 gggctgggcg ttaatgttag ttggtgctat ccgtttaaag cgcaagggcg taagtcatga
     6001 ataaggttgt attgctgtgc cgtccgggct ttgaaaaaga gtgcgccgca gaaattaccg
     6061 ataaagccgg ccagcgggaa attttcggtt ttgcccgcgt gaaagagaat tcgggttatg
     6121 tcatttatga atgttatcaa cctgatgatg gcgataagtt aatccgtgag ctgccgttca
     6181 gttcattaat ttttgcccgc cagtggtttg tggtggggga actcctgcag catttgccgc
     6241 cagaagatcg tattaccccc attgtcggca tgttacaggg cgtagtagag aagggcggtg
     6301 aactgcgtgt tgaagttgcc gataccaacg aaagcaaaga gttgctgaaa ttctgccgta
     6361 aatttactgt tccgctacgc gctgccttgc gcgatgccgg ggtgctggcg aactatgaaa
     6421 cgccgaagcg tccggttgtg catgtattct ttattgcacc aggctgctgc tataccggtt
     6481 actcatacag caacaataat tcgccgttct atatgggcat tccgcgcctg aaatttccgg
     6541 cagaggcgcc gagtcgttcc acgctcaaac tggaagaggc atttcatgtg tttattcctg
     6601 cggatgagtg ggatgaacgc ctggcgaacg ggatgtgggc ggtggattta ggcgcttgcc
     6661 ctggcggctg gacctaccaa ctggtgaagc gcaacatgtg ggtttattcc gtcgacaacg
     6721 gcccgatggc gcaaagtctg atggataccg ggcaggtgac gtggctgcgg gaagacggtt
     6781 tcaaattccg tccgacacgc agcaatatct cctggatggt atgcgatatg gttgaaaaac
     6841 cggctaaagt tgcggcattg atggcgcagt ggctggttaa tggctggtgc cgtgaaacca
     6901 ttttcaatct caaactgccg atgaaaaaac gctacgaaga agtatcacac aatctggcgt
     6961 atattcaggc acagcttgat gaacatggca taaatgctca gattcaggcg cggcagttgt
     7021 atcacgatcg cgaagaagtg acggtgcacg tccgccgcat ctgggctgcg gtgggtggtc
     7081 gtcgcgacga gcgataacag gcaaggccgg ggcgtcgtct tcggcctgct accctttccg
     7141 tcaggctgtt accaaagaag ttgcaacctg gcgctcatca gaaataatgt gcgttacctc
     7201 atccagatgc ccgatgcggg cttcccctga acgattaaat ttacttttat caatcaataa
     7261 caacgattgc gcggcacgtt ttaatagcat cgatttgtaa tcagcgttga tcgcattgga
     7321 gtcccacagt gcgccgctgc tatcgatccc ttcacaagaa aaaataaaca gatcgatttc
     7381 cagcgattta agttgggaaa tcagcgaggg attgacgtaa cagccatatt tgcgctcaag
     7441 tgtgccgccg gaactgatca gttgaatgcg ttcgcgttta ccgagttcat agcaaatcgg
     7501 atggctattg gtgaagacct gaatgttgat gtcaggcaac tggcgtgcca gataccagca
     7561 agttgaactg gcgtctaagg ctatcaccat cccttcttca atccacgcga gcgcctcgcg
     7621 cgcgatatct gctttatgcg cataatggct tttcagcctg atgtgaaagg gatcgccact
     7681 gtcttgattt tgacggtgga tatatttagc gcgtccatga ttgcgcagaa ttttaccctg
     7741 cgtctgtaat tcattgagat cgcgacgaat ggtttcttta ctgaccttta gctgttcaga
     7801 gagagcttcc gtggtcaggc tggtatggtt cagcagcagg tcgactatcg cttgctggcg
     7861 tgccgctttc atatttttca ccccatcatt cgataaccgc ccgcgtcatg cgggcgtacc
     7921 ggtatgagat tacggtgtta cccctttttt taaaagaata ttcccgtact tcgctcgttc
     7981 gcctgtgata acgatcgcaa aggctttttg cgcccgttca taaaacgcaa aacgattgat
     8041 gcggatgatg tcaggacacg gggcttgtag tgaaagcgca ttacggtaac gtcgttctac
     8101 ttcaggatcg agagtgtcac cttctaccgc cgccatcatc accagcggcg gtgcataact
     8161 gtccagttca aataacggga taatcgcctg gagcaagtcg ctcaccaaca ggccatcagc
     8221 gcggatcacc tgcggtccca tcgaatgggc gggaaagtga gcatcggaaa aaataatttc
     8281 atctccatgt cccatctctg ccagcacttt caatagttcg ggagaaatta acggcgaaat
     8341 tgttttcagc atttcacact tcctctataa attcaggttc agtttgcggg tagaaataac
     8401 ggtactgata atgaatctgt gcgcgggctt cttcagggct gttaaattcc cctacgccat
     8461 accaaccgaa cagcgcagct cctgcgacgg tcgtttcggc gtcgtcgaga acttttaccg
     8521 gaatatcaag catattggct ttaatctgat tccacaatgt gttgcgactt cctccaccga
     8581 ctaacaataa ttcagaggcc ttaaagtgcc cgattttttc cagcatctgt agattgcgct
     8641 gtaattgcgc agttaaccct tccagcgccg cgcgatagaa atgcccccgc gtggtattaa
     8701 gcgtcactcc ttgccagcca gcgttctgac acgacaataa atcacactgc atttttacgc
     8761 catccgcgct aggcgcgata agacgagctt cttcaatcaa catttgccag ggtgtttcag
     8821 ccgtccagaa cagttttctc acccattcca gcacgccgga tgccagccat tgcatacctg
     8881 ggttatacaa ccctgcctgg ctatccagtt cgcaggtgga accggcgtac tgacttaaca
     8941 gcgaagtatc aacctgggcg ctacggacta ttaaaatttc ccatgtaccg gaagagagca
     9001 cgggttcatt ttggtcggca ccagcgccaa aaagggcgaa ctgggtatcg tgacctgcgg
     9061 aaatcaccgg tatgccaacg ggtaagccga gcattgctgc ggcgctgttc tgtagcgtac
     9121 caatctgttc acccgcttcc accagacgag ggaagagtcg gcgtggaata ccagtggctt
     9181 gtaaaatttg cggactgaaa tcgcgttgct ggatatccag catctggctg gttccggcca
     9241 tcgtgatatc agtagtgaat tcgccggtta aacggtggtt aatcagcgac gaaataaaga
     9301 accaggcgtg cgtgcgttcc agcagttgtg gatgattttc tttcaaccac accaacttat
     9361 ataacgtatt gaaactaaag gctccgacgc cagaaatagc ctgcaaccgc tgtgcggaga
     9421 tcaactgttc aatattgtcc ataaccgctg ctgttcgcgg acatttccag ctaatgatcg
     9481 gatagagcag attgccttgc ttatctacca gagcgccatc cacaccaaag gtggtgacgg
     9541 cgataccgcg gatgtggcat tcagtcagtt cactattgat ttgccgacag caatcagcaa
     9601 agcgttgcaa aatggcgtct aaagaccact ggtgccaggt gttgttttcc atcgcgatat
     9661 cgctggcatt aggcgttgag gcgcgggcaa caatttttcc ctgccgatta accgcgatgg
     9721 ccctgacatt ggtcgcgcca cagtcgagta ccaggataac ttcttgtttc ataatcgctc
     9781 ctgcaatact tcaatatcca tgccgtgcgc agcccaggca gaaggacgat acactttggt
     9841 ctcttcaacg ttgtgcatac ataccgggat acgcagcatg gaggcgagag tgataaagtc
     9901 agcgccaacg tggccgatgg tcagaacccc atggttagcg ccccagttcg ccattaccga
     9961 gtacacatcc gtaaacggcc ctttaccggt gaggcgcggt gcaaaccagg tggttggcca
    10021 ggttgagttg gtgcgtttgt tgaggatgtc atgcacatcc ttcggcaatt ccacgctcca
    10081 gccttccgcg atttgcagta ccggtcccag gcctttgatg atgttgacac gagtcatggt
    10141 gaacgggacg ccgccttcgg taaggaagcg ggaagagtaa ccgccgccac ggaagtattc
    10201 gtggatcgcc gggcaccatt cggtagcggc gaggcaagcg tcagcctctt gctgagagat
    10261 ttcccagtgc ggcttcatcg tcgggttacc ttcgctgtcg cgttgtttac aggaaccgtc
    10321 cagcgcagca gaacctgagt tgatcaaatg gatgatgccg tgttctgcca gtccatcgag
    10381 tttatgcccc gttacacgct caattgcttc tggtgaccag taggtacgca catcggcaaa
    10441 tacctgagcg gtgccggtga gctggtgacc cattagcatt gccacgccgt taagactgtc
    10501 gttttcggtc gccacgacaa agggttcgcg cacgccattc cagtcaaatg aactgttgag
    10561 gatcgcttcg gcggtgtcac cattgggata ttgatcggtc cagtgacgtt gcccctggaa
    10621 gcccgcagcg atggcgttgt agccaagtga ttcttccacg cgaccaatat cggccagttt
    10681 gctgttgcct tgcatcatgt cgcggataca catcgccatc agtaaacttt cgcgcagaac
    10741 tgcgcggctt tgctcggcat tgcgttgata ctgtttgtta ttttcatctt cgccatagcg
    10801 gaagttttta tcagcccagg ccagtgccat ttccagttcg gcttcgtcgt aaatcttctg
    10861 atcgatacgg cgacgcagtt cggtcatatc caccgcctgg actttcattc ccagccagga
    10921 ttcaaagaag ttgtgatcaa cgatggaacc ggcgataccc atcgaaacgc cgcccagcga
    10981 cagatagctt ttacctttca tgctggcgac ggccaaaccg gcgcgggcaa agcgcagcag
    11041 tttttcttca acatcggcag gaatcgatgt gtcatcggca tcctgaacgt catgaccgta
    11101 aatggagaat gccgggatgc ctttctggct gtgagctgcc agagccgctg ccaggtaaac
    11161 agcgccggga cgttcagtgc cgttaaagcc ccaaatggct ttcgggcggg ttggatccat
    11221 gtcgatggtt tcactgccat agcaccagca aggcgttacc gtaatggtga ggcctacatt
    11281 ctggctgctg aatttttctt cgcaagcagc ggcttcagcc atacccgcga tacaggtatc
    11341 ggaaatgaca cactcgacgg cagctccgca ggcatggcgc agtttctcgg tcagcagtgc
    11401 ggccgtagct ttcgccatat tcattgtttg ttcttcaagc gactcacgaa cacccatgcg
    11461 acgaccgtca ataaccgggc ggataccaat tttcggtaag ctgatttttt tcattacaga
    11521 ttcctcactt tattcggaaa atatgttcag ttagttgccg tttgagagcg gaaacgggca
    11581 aagataaaga tgaccgcgaa gcagagtgcg gggatcagtt cagcagtggg gatgttgccc
    11641 gccgcgtcac tgacaaaacc catgaccgga gtgacaatac cgccgccaat aatggtcata
    11701 acgatgaagg acgaaccgta tttggtgtcc tggccgagat tcttaatgcc cagcgagaag
    11761 attgttgggt actgaatcga cataaaggcg ctgcataaag tcagggctat taagcccaca
    11821 tgaccgccag cgaaggctga gatcaggcac agtgccatag cgattaatgc gtaggcggcc
    11881 aggactttgt gtggtgcgaa gcgactgatg agccaggtac cggtgaaacg accaataaag
    11941 aagcacacca tggttccggt taaatagtta gcggcaaagc ctgcagtcat acctggaatt
    12001 tcttctacag cgtagcgaat caaatagctc cagcaggccg tttgtgcgcc aacgtagcag
    12061 aattgcgcta atactgccca gcgccagtgg cgaatacgcg ccaggcgaga aagcgatgcg
    12121 gagaacgatc cttgtttggc gtcactgtga ttatcactct gcaatgccgg gaatttcgtc
    12181 agcatgatca gcagggcgac cagtaacacg atagccacga tgatcatata aggtgtctgt
    12241 accgataata ccaggctgtg tttatacgca ctcaattgct ctggagacat tttatcgaga
    12301 acgtcttgcg attgatgtgg cacgttagac aaaataagac tttgcccaaa gacaaccgcg
    12361 ataattgcgc caaacgagtt aaatgtttgc gcaagattta agcggaagtg accactgctt
    12421 tccggcccta ataccgtaac aaaagggttt gcggcagttt ccagacaacc taatccggct
    12481 gcaataataa ataggccaac taaaaataag gtgtagttca ttatttctgc agcgggccag
    12541 aataatgcag cgcccagggc atataaaaat aac
//
LOCUS       DAJTNN010000121        12511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTNN010000121 DAJTNN010000000
VERSION     DAJTNN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.121"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..456)
                     /locus_tag="OO150_002931"
     CDS             complement(<1..456)
                     /locus_tag="OO150_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5517006.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETG"
     gene            complement(486..1202)
                     /locus_tag="OO150_002932"
                     /pseudo
     CDS             complement(486..1202)
                     /locus_tag="OO150_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            1498..2274
                     /gene="exuR"
                     /locus_tag="OO150_002933"
     CDS             1498..2274
                     /gene="exuR"
                     /locus_tag="OO150_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5517007.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            2619..3281
                     /locus_tag="OO150_002934"
     CDS             2619..3281
                     /locus_tag="OO150_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5517008.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            3285..3668
                     /gene="mzrA"
                     /locus_tag="OO150_002935"
     CDS             3285..3668
                     /gene="mzrA"
                     /locus_tag="OO150_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5517009.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3815..4183
                     /locus_tag="OO150_002936"
     CDS             3815..4183
                     /locus_tag="OO150_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR5517010.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            4221..4526
                     /locus_tag="OO150_002937"
     CDS             4221..4526
                     /locus_tag="OO150_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5517011.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            4529..4933
                     /locus_tag="OO150_002938"
     CDS             4529..4933
                     /locus_tag="OO150_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5517012.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4923..5222
                     /locus_tag="OO150_002939"
     CDS             4923..5222
                     /locus_tag="OO150_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5517013.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            5408..5800
                     /locus_tag="OO150_002940"
     CDS             5408..5800
                     /locus_tag="OO150_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5517014.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSKFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5870..6773
                     /locus_tag="OO150_002941"
                     /pseudo
     CDS             5870..6773
                     /locus_tag="OO150_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            7066..7431
                     /locus_tag="OO150_002942"
     CDS             7066..7431
                     /locus_tag="OO150_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5517015.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7673..8029
                     /locus_tag="OO150_002943"
     CDS             7673..8029
                     /locus_tag="OO150_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5517016.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGVWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(8080..8976)
                     /gene="yhaJ"
                     /locus_tag="OO150_002944"
     CDS             complement(8080..8976)
                     /gene="yhaJ"
                     /locus_tag="OO150_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5517017.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            9081..9782
                     /locus_tag="OO150_002945"
     CDS             9081..9782
                     /locus_tag="OO150_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5517018.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9805..9969
                     /locus_tag="OO150_002946"
     CDS             9805..9969
                     /locus_tag="OO150_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517019.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(10103..11413)
                     /locus_tag="OO150_002947"
     CDS             complement(10103..11413)
                     /locus_tag="OO150_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5517020.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLEDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11441..>12511)
                     /locus_tag="OO150_002948"
     CDS             complement(11441..>12511)
                     /locus_tag="OO150_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517021.1"
                     /translation="IRWQNHTECKDYPSVISGYLGKNWGILLGALYFVMLVIWMFVYS
                     TAITNDSASYLHTFGVTEGVLSDSPFYGLVLICILVAISSRGEKLLFKISTGMVLTKL
                     LVVAALGVSMVGMWHLYNVGSLPPLGLLVKNAIITLPFTLTSILFIQTLSPMVISYRS
                     REKSIEVARHKALRAMNIAFGILFVTVFFYAVSFTLAMGHDEAVKAYEQNISALAIAA
                     QFISGDGAAWVKVVSVILNIFAVMTAFFGVYLGFREATQGIVMNILRRKMPAEKINEN
                     LVQRGIMIFAILLAWSAIVLNAPVLSFTSICSPIFGMVGCLIPAWLVYKVPALHKYKG
                     MSLYLIIVTGLLLCVSPFLAFS"
BASE COUNT         3199 a         3158 c         2989 g         3165 t
ORIGIN      
        1 gcctgtctcc agaacacctt tggtggtttg agcaaacatc aggtttttag ccagagaacc
       61 gttagttaac gcatcggaag caaagatttt ggttgcggta aaagtggttg ccgcatcagc
      121 tttaatttct tcaccgcccc aggttactgt cagcggaaca atcttgctag catccaggct
      181 ttgaaccagt tgcgccgggg aaactaactg catttttaca tctttggtaa cgtcgttaga
      241 ccagattttg gtcatcaact ggtaagattg cagaccctgc cccggcagat attgcatttc
      301 tactgctttc ggcaacgcgg tgttatcggt ctgtgtcata tccagagcgg catcaacgtt
      361 ggccgttaca gtaatatcct tttgtactgc caatgcggag cctgcaacag aaaacatcgc
      421 tgcgaccaga agagattttg caaatacctt tttcattact attccttcgt ttattacaat
      481 gataattaat ggaccgccag ttcaaactcg ccattatctt tggtgatcca gttattatat
      541 ttgacaacga ttttgtcgta gccagatatg ctttcaagat ttctttcctg accagggaag
      601 atgtttttat ttacggtctg tatcttacaa ttttctttgc tattattttt acaataagcg
      661 atcgttaaaa tctttaagcg ttgattccct gcattaacta atttttgggc agcatttact
      721 tctaacttag caataggttg ctgaggtgga acactaacaa caatccccca aattaagcta
      781 acagttaact gcatagagaa atctttttta tctgtggcaa cgtcatcaat actgggaacc
      841 gcttcaaatt taacgcgtta atttttttct ttttgctctg gttccattgc aacaaagcgc
      901 acagttttgc tactaccggc tggtaaagcg aatttaggtg gcataacaac cagatgattt
      961 gcatcaccgg atttaatctc aacttcattt tcctgaggcg tcgatggatt atcgatacgg
     1021 aatactgtcg ccttgatgta ctgaacttca ttacttttag atattacgcg aacgttgcct
     1081 tcatcctgac tattaatgga aacagccata ggataaacag tcatatttga ctgtgccaaa
     1141 aaaggagcga acacgaataa aagcggcgca agccgtgata agagattaat tttcgccacc
     1201 atataaattt gatcttacaa tattgtttcc aaaaagttgg tgtattagag atgtagattt
     1261 attgtaagtc aatataaaaa agcattttta gttaatatta aatttaaagg ctgggaatta
     1321 acttgctgaa atttagtagc tttcacaatg acgatactta ttttttagga atttataaat
     1381 attcacaata ttaattcaat acattaattc aatcaccaaa tttatatatg accaatggca
     1441 tacccatgaa taaagtggta tgataacttc gtccgcattc cactccggga gacgcatatg
     1501 gaaatcactg aaccacgccg tttgtatcaa caacttgccg ctgacctgaa agagcgcatt
     1561 gaacagggcg tctatctggt gggtgataaa ctgcctgccg aacgctttat tgccgatgaa
     1621 aagaacgtca gccgaacggt agttcgtgaa gccatcatca tgctggaagt tgaaggctat
     1681 gtggaggtgc gtaaaggttc aggtattcat gtggtttcca accagccgcg ccatcagcag
     1741 gcggctgaca ataatatgga attcgccaat tacggtccgt ttgagttgct tcaggctcgc
     1801 cagctcatcg aaagtaatat tgccgagttc gcggcaactc aggtaacgaa acaagacatc
     1861 atgaaactga tggccattca ggaacaggcg cgcggcgaac aatgcttccg tgattccgag
     1921 tgggatttac agttccacat tcaggtagcc ctggcgacgc agaactccgc cctggcggcc
     1981 atcgttgaaa aaatgtggac ccagcgtagc cataacccgt actggaaaaa actacacgaa
     2041 cacattgatt cccgtaccgt tgataactgg tgtgatgacc acgatcaaat tctcaaggcg
     2101 ttaattcgca aagatcccca tgctgctaag ctggcaatgt ggcagcatct ggaaaacacc
     2161 aaaatcatgt tatttaacga aaccagcgac gatttcgagt tcaatgccga ccgctatcta
     2221 ttcgccgaaa acccggtagt gcatctcgat acggccacca gtggcagtaa atgaaactcc
     2281 tgtccgacag gcgctgcgac gcgcctgttc gcttcgtcgg taagcgaaaa gtataaagtg
     2341 tcagcctgtg taaatcctct cgccaccctc ccctgcattc agcaaaatca gactccacgg
     2401 acatgtaatt ttgataacga acaacgttga cctttgttac aattagattc aatttgaatt
     2461 tatgtttttg aatgctttct tatctcacga tttaacaggg aatagttcag gctgtgttga
     2521 tgtatcaaac ccgcagaaca taccaaaaca gcaataacat tgcggtagtg catcttttaa
     2581 aaccagcgtg gcgttaaccg attcaccagg aataatgaat ggaacttttg acccaattgc
     2641 tgcaagccct gtgggcgcag gattttgaaa ccctggccaa tccatcgatg attggcatgt
     2701 tgtattttgt cttgtttgta attttgttcc ttgaaaacgg cttgcttcca gcggcctttt
     2761 taccgggcga cagtttactg gtattggtcg gcgtgttgat tgcgaaaggc gcgatgggtt
     2821 acccgcaaac gattctgctg ctgaccgttg ccgccagcct cggctgctgg gtcagctata
     2881 ttcaggggcg atggctgggc aatacccgca ccgtacaaaa ctggctatct catttacccg
     2941 cgcattatca tcaacgcgca caccatcttt ttcataaaca cggtttatcg gcgctgttaa
     3001 ttggtcgctt tattgcgttt gtcagaacac tgctgccgac gattgccggg ttatcagggc
     3061 tgaataacgc gcgctttcag tttttcaact ggatgagcgg tctgctgtgg gtattgatcc
     3121 tgacaactct gggttacatg ctcggcaaaa cgccggtatt tttaaagtac gaggaccagc
     3181 tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt gtttggcctg gcaggttctc
     3241 tggtcgtgtt atggaaaaag aaatatggaa atcgggggta agggatgcaa atacctcgca
     3301 tgtcgcttcg ccagctagcc tggtccggca ctgttttact tctggtcggc acgctgttgc
     3361 tggcctggtc cgcggttcgc cagcaagagt ctacgctggc gattcgtgcc gttcatcaag
     3421 gcacaacgat gccagacggt ttttcgatct ggcatcacct tgacgctcat ggcattcctt
     3481 tcaaaagtat cacccccaaa aacgacactc tgttaattac atttgactcc agcgaccaga
     3541 gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca tggctacatc attgcgcagc
     3601 aggacaataa cagtcaggct atgcagtggc tgacccggtt acgggataac tctcatcgct
     3661 tcggataact tcctgttatc cgaaacatat cactcacttt ggtgatttca ccgtaactgt
     3721 ctatgattaa tgaggcggtg gttaacacta ccgcttcgtt gtctggacaa tcgttccttt
     3781 gtaacaggtc cgaatcacaa tggaaggttc aagaatgaaa taccgcatcg ctttagctgt
     3841 ttctctcttt gctcttagtg ccggtagtta tgccactacc ctgtgtcagg aaaaggagca
     3901 aaatatcctt aaggagatca gctatgccga aaaacaccaa aaccagaatc gtattgacgg
     3961 tctgaataaa gccctgagtg aagtccgggc caactgttca gatagccagc tgcgtgccga
     4021 tcatcacaag aaaatcgcaa agcagaaaga tgaggtggcg gaacgccagc aagatttagc
     4081 cgaggcgaag caaaaaggcg atgccgataa gattgccaaa cgcgaacgga aactggcaga
     4141 agcgcaggaa gagctgaaaa agctggaagc gcgcgactac taactcacaa tagtcactac
     4201 ttactcacct ggagaaaact atgtcgaaag aacacactac ggaacatctg cgtgctgagt
     4261 tgaaatccct ttccgatacg ctggaagagg tgcttagctc atctggcgag aagtcgaaag
     4321 aagagttgag taagattcgt agcaaagcgg agcaggcact gaaacagagc cgttatcgcc
     4381 tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt cgcggcggcg cgtgctgatg
     4441 agtatgtgcg tgaaaatccg tggaccggcg tgggaattgg cgctgcaatc ggtgtagtgc
     4501 tcggcgttct gctgtcgcgt cgttaattat ggcggacact catcacgcgc aagggcccgg
     4561 taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc atggttgaaa tggtggaaac
     4621 acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa gctaatctct ttcaactttt
     4681 actgatgctg ggcctgacga tgcttttcgc tgcatttggt cttatgagcc tgatggtgct
     4741 aattatttgg gcggttgatc cgcaatatcg cctgaatgcg atgattgcca ccaccgtggt
     4801 gttgctgcta ctggcactga ttggcggtat ctggacgtta cgtaaatcgc gtaaatccac
     4861 cttgctgcgc catacacgcc atgagctggc aaacgatcgg caactgctcg aggaggagtc
     4921 ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca actgcttagc cagatccagc
     4981 aacaacggct ggatctttcc gccagtcgtc gtgaatggct ggaggcaaca ggcgcttacg
     5041 atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc gctggttggc agtagcgtga
     5101 tggcgatctg gacgattcgc catcctaata tgctggtccg ctgggccaga cgcggttttg
     5161 gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa gcagcaacag cttcgcggtt
     5221 aacttccctc tggccggagc cattccggcc ctatcactca aattttttga agatttttga
     5281 cagttttcct tgctaacaat catcattcac cacgtttatg attctctcca tcgacagcaa
     5341 cgacgctaat accgcgccct tgcacaaaaa aacaatcagc agcctgagtg gcgcagtgga
     5401 gagtatgatg aaaaaattag aagatgttgg tgtactggta gcgcgcattt taatgccgat
     5461 tctgtttatt accgcaggct ggggaaaaat caccgcttac tctggtaccc aacaatatat
     5521 ggaagcaatg ggtgtcccgg gttttatgct gccgctggtg attctgctcg agttcggtgg
     5581 tggtctggca atcctgttcg gtttcctgac tcgcaccaca gccctgttta ctgcgggctt
     5641 tacgctgctg acggcatttt tatttcacag caagtttgct gaaggcgtca actcgctgat
     5701 gttcatgaaa aacctgacaa tttctggcgg attcctgctg ctggcaatta ccggtccggg
     5761 cgcgtatagc atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta tacttaacga
     5821 taaaaaacga ggaggaagct cctcgttttt gctattggag gagagaaaaa tggggcaact
     5881 gattgacggc gtctggcatg acacctggcc ccactggcac aggcggtttt atcgcagaga
     5941 aagatcgtta tcatctctat gtttcactcg cctgcccgtg ggcgcaccgc acgctgatca
     6001 tgcgcaaact caaaggactg gaaccgttta tttccgtttc cgtagtgaac ccgctgatgc
     6061 tggaaaacgg ctggaccttt gatgacagtt ttccgggagc aaccggagat acgctctatc
     6121 aacatgaatt tctgtatcag ctttatctcc acgccgatcc acactacagc ggacgagtta
     6181 ctgttcccgt gctgtgggac aaaaagaacc acaccattgt cagcaacgaa tcagcagaaa
     6241 tcatccgcat gttcaatacc gcgtttgatg cgctgggcgc aaaagcgggt gattactacc
     6301 caccagccct gcaaacgaaa attgacgaac ttaacggctg gatttatgac accgttaaca
     6361 acggcgtgta taaagctggt tttgccacca gccagcaagc ctacgacgag gcggtggcga
     6421 aagtgtttga atcgctggcg cgactggagc agattttagg ccagcatcgt tacctgaccg
     6481 gcaaccagct aaccgaagcc gatattcgcc tgtggactac gctggtgcgt tttgatccag
     6541 tgtatgtgac ccacttcaag tgtgataagc accgcatcag cgattacctg aatctgtatg
     6601 gcttcctgcg cgatatctac cagatgccgg ggatcgccga aactgtcaat ttcgatcaca
     6661 tccgtaatca ttacttccgc agccataaga ccatcaaccc tacggggatt atttcaattg
     6721 gtccgtggca ggatctcgat gaaccgcatg gacgagatgt tcgcttcggt taaaaaaagg
     6781 caccttacga ggtgcctctg ataattagct aagtgcatgt tgttcatgtc ggatgcggcg
     6841 tgaacgcctt atcaaaccta caaaacgttt atattcaata tgttaaaatg accgcgtagg
     6901 ccagataagc gtagcgcatc tggcgatttt gcgcttgtca tcagtcttag cgcaccttca
     6961 cggcacttta ttaattcctt gaacgaatat ttactgccat ttcattcacg tctattctta
     7021 atttgctgct ttaaaaacaa gtgattgagc aaaattgagg caaaaatgga ctggtatctg
     7081 aaagtactaa aaaattatgt cggtttccgg ggtcgtgcgc ggcgcaaaga gtactggatg
     7141 tttattctgg tcaacatcat ctttacgttc gtgctggggc tgctggataa aatgttaggc
     7201 tggcaacgcg ccggtggcga aggcatcctg acgacaatct acggtattct ggtgttttta
     7261 ccgtggtggg cggttcagtt ccgccgcctg cacgacaccg accgctcggc gtggtgggca
     7321 ctgttgttct taatcccgtt tatcggctgg cttattatca tcgtctttaa ctgccaggcg
     7381 ggtacgccag gcgaaaaccg ctttggacca gatccgaaac tggaacctta aaacaaatct
     7441 gctcgccgga tgtctgactc atccggcttt tatcatcatg ttgatgaatg aataatttac
     7501 taatgtttat ttaaaatatt tcaatgtcgt tatataaatt acctataaaa aacaaccatg
     7561 tatttatctt aaatatcaaa acctttataa catccgaaaa agaaagatac gaatagcatt
     7621 tacacaatac ccggaataga ctttcgtcac ttttaattaa agggatgttt ttatgcagtg
     7681 gtatttagcc gtattaaaaa attatgttgg tttctctggt cgcgcacgtc gtaaagagta
     7741 ctggatgttt actctgatta acgccatcgt cggcgctatt atcaatgtca ttcaattgat
     7801 tttaggttta gagtttccat tcctttctct catttacctg gcagcaacaa tcattccggt
     7861 cattgctctg tgtgttcgtc gtctgcacga tactgaccgt tcaggtgtgt gggcgcttct
     7921 ctatctggta ccgatcattg gttggttagt tctgtttgta tttgcctgtt tagaaggcaa
     7981 ttctggcagc aatcgttacg gaaacgatcc taagtttggt tcaaattaat cttagaattg
     8041 gggcgatatt tcgccccttt ttattaacaa taataaatat tattttccgc taaaaagttt
     8101 gggaatttcc cgcagacacc aggatttcgc ttcccccata ctgtcacgac gccaggccat
     8161 aataatatcg atctcgctgg tcgattccgg gctgacgaca cgcaaccgcc cttccgcaat
     8221 atctttttcg accatcggat acggcatcgt cgccacgcca agccccgcca gtaatgcctg
     8281 acgtttatct tcaatcgtgc tcaccgttaa gcgcggctgt ttgtccagca gctgtacggt
     8341 caacaccggg cgctcacgag cggtatccgc taccgcaatt ccacgatatt tcacgcgcgt
     8401 cacttcagat aacggttccg gctcctgatg aatcgggtga tcaggcgcgg caacgtagac
     8461 gttcattaag gtatagagct tgcgcgagtt gatctccgac gaggaacgaa aatgcatatc
     8521 cggcgcgata acgatatccg cccgcccctg ctccagtcgt tcccacgccc ccgccagcac
     8581 ttcggtgatg attgccagtt gggtattggc ttttgccgcc agtttgtcga ttaacgggaa
     8641 aaaggcaggt gtcggtacca gcgcctcggt cacaatggtg agatgcgttt cccaaccgcg
     8701 cgcgagggct tccgcatcgg tagtcagttt atctgctgct tccagcaaaa cgcgccctcg
     8761 ctccagcaac atcctcccga cattggtgaa tttggtgcga tggcccgagc ggtcaaacag
     8821 caccacatcc agctcttctt ccagtttttg catggtgtag ctaagtgcgg aaggcacgcg
     8881 tcccagctca tccgccgccg ccgcaaaact gccccggcga tcgatcgcat ccataacccg
     8941 tagtgcttcc agcgttaatg ccctttcttt ggccatttcg ttctcattca ggaaatttga
     9001 acataccggg cagaatatct ggctaacaat gtagcgtcca gccccttacc ataaaaggaa
     9061 gtaaagagag gtcaagaatt atgattacta cccgaactgc caggcagtgt ggacaagcag
     9121 actacggatg gttgcaggcc cggtatactt tttcctttgg acactacttc gacccgaaat
     9181 tgttaggcta tgcctccctg cgtgtgctta accaggaagt gctggcccca ggtgccgcct
     9241 ttcagccgcg aacctatccc aaagtcgata ttttaaatgt gattctggat ggagaagcag
     9301 agtatcgcga cagcgaaggc aatcatgttc aggccagcgc cggtgaggcg ttgctgctct
     9361 ctacccagcc gggtgtcagc tacagcgaac acaatctcag caaagacaaa ccgttaacac
     9421 gaatgcagct ttggctggac gcctgcccgc agcgagagaa tccgctgatt caaaaactgg
     9481 cgcttaatat gggcaagcag caattaatcg cctcgccaga gggtacgatg ggaagcctgc
     9541 aattacgcca gcaagtgtgg ctgcaccata tcgtgctcga caaaggcgaa agtgcgaatt
     9601 tccagttgca tgggccacgc gcgtatttgc aatcgattca cgggaaattt catgcgctta
     9661 cgcatcatga agagaaagcg gcgctgacct gcggtgatgg ggcgtttatt cgtgacgagg
     9721 ctaacattac gctggttgcc gattccccac tgcgcgcttt gctgatagat ttgcctgtct
     9781 agttgttttt acagggagat gatgatgagt aaaaaatcgg ccaaaaagcg ccagccagtg
     9841 aagcccgtgg tggcgaaaga acctgctcgc accgccaaaa attttggcta tgaagagatg
     9901 ttgagcgagc tggaagccat cgtcgcggat gctgaaacgc gtttagccga ggatgaagct
     9961 accgcgtaaa gacaggttgc cggatgcggc gaaaacgcct tatccggcct acaaaatcca
    10021 acaacattaa cgaattaaac aacccgtagg ccggataagg tgcgccagca tcgcatccgg
    10081 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
    10141 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
    10201 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
    10261 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
    10321 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
    10381 cataacgccc atcctccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
    10441 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
    10501 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
    10561 ccacaggcat tgttgcggtg atcccctggt tacccgagcc ggagttactc atagccggaa
    10621 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
    10681 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
    10741 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
    10801 gaataaagcg gatcgccgca aacgggactt cattgacgaa cttcaggatc tcagccagcg
    10861 tcgttctgga aagcaccgtc agcggagact cttgctcgcc ctctgccaca cacgcctgct
    10921 gggtaaacac cacactattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
    10981 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
    11041 tgcaaggttc ctggatctta acggagactt tccccgccgc cagcagtgct ttggcatcgg
    11101 caattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
    11161 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
    11221 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
    11281 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
    11341 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
    11401 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaacg
    11461 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
    11521 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
    11581 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
    11641 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
    11701 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
    11761 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    11821 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    11881 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    11941 cgtagaaaaa gacggtgaca aataaaatgc caaacgcgat attcattgcc cgcaatgctt
    12001 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    12061 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    12121 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    12181 acacgcccag cgcagcgact accagcagct tggtcagcac catgccggtc gaaattttga
    12241 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    12301 aaaagggact gtctgacagc accccttccg tcacgccgaa ggtatgcaga taggaagcac
    12361 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    12421 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    12481 ctttacattc tgtgtgattc tgccagcgta t
//
LOCUS       DAJTNN010000122        12323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTNN010000122 DAJTNN010000000
VERSION     DAJTNN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.122"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(287..625)
                     /gene="osmE"
                     /locus_tag="OO150_002949"
     CDS             complement(287..625)
                     /gene="osmE"
                     /locus_tag="OO150_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5517022.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            827..1654
                     /gene="nadE"
                     /locus_tag="OO150_002950"
     CDS             827..1654
                     /gene="nadE"
                     /locus_tag="OO150_002950"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5517023.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            1884..2771
                     /gene="cho"
                     /locus_tag="OO150_002951"
     CDS             1884..2771
                     /gene="cho"
                     /locus_tag="OO150_002951"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5517024.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(2731..3306)
                     /gene="ves"
                     /locus_tag="OO150_002952"
     CDS             complement(2731..3306)
                     /gene="ves"
                     /locus_tag="OO150_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5517025.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3509..3979)
                     /gene="spy"
                     /locus_tag="OO150_002953"
     CDS             complement(3509..3979)
                     /gene="spy"
                     /locus_tag="OO150_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5517026.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4309..5277)
                     /gene="astE"
                     /locus_tag="OO150_002954"
     CDS             complement(4309..5277)
                     /gene="astE"
                     /locus_tag="OO150_002954"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5517027.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5270..6613)
                     /gene="astB"
                     /locus_tag="OO150_002955"
     CDS             complement(5270..6613)
                     /gene="astB"
                     /locus_tag="OO150_002955"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR5517028.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6610..8088)
                     /gene="astD"
                     /locus_tag="OO150_002956"
     CDS             complement(6610..8088)
                     /gene="astD"
                     /locus_tag="OO150_002956"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5517029.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKLRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGRANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQLETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8085..9119)
                     /gene="astA"
                     /locus_tag="OO150_002957"
     CDS             complement(8085..9119)
                     /gene="astA"
                     /locus_tag="OO150_002957"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5517030.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(9116..10336)
                     /gene="astC"
                     /locus_tag="OO150_002958"
     CDS             complement(9116..10336)
                     /gene="astC"
                     /locus_tag="OO150_002958"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR5517031.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIMAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGDFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            10782..11588
                     /gene="xthA"
                     /locus_tag="OO150_002959"
     CDS             10782..11588
                     /gene="xthA"
                     /locus_tag="OO150_002959"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5517032.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            11755..>12323
                     /locus_tag="OO150_002960"
     CDS             11755..>12323
                     /locus_tag="OO150_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5517033.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITL"
BASE COUNT         2981 a         3343 c         3035 g         2964 t
ORIGIN      
        1 catcgacgat tatctgtcag ccagacactc cgcaagcctt aacctgcttc catgctctgg
       61 gtaacttgcg aaaccaacat gacttttttt gtggctgaaa ggagtatacg gtatagacct
      121 gagcaatagt atttctttga ccttataaat tgtctctcct tgacctgcgg ttatgaccct
      181 cttcacattt cgggcaaata attcgcgaag ataattaaga actcaggcca aaaaaaccgg
      241 cgcaatggcc ggtttccgtt gttactcaag ctttcaaacg aagtgattac ttcgcagcct
      301 gtggatcagt gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat
      361 catctaacgc gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct
      421 ggcaagtacc acgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg
      481 caacctgcgc ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt
      541 ctttggtacg atcataagcc gtacaacccg ccagcatggt taataccgcc gctgcactca
      601 gaattcctgc catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag
      661 cctggaatac attgggcgct ttttcaagcc cgtgaacgaa acggctccgc tttcagagga
      721 ttcctgtatg acgttttatc caccattcaa cccgctgtcg cttgtcgttt cagtagcgac
      781 gggttagctt taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca
      841 acaaataata aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg
      901 tagtgtcgat tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct
      961 cgggatcagc ggcggtcagg actccacgct tgccggtaag ctgtgccaga tggcgattaa
     1021 tgagctgcgc caggagaccg gcaacgaatc actgcaattt attgccgtac gcctgcccta
     1081 tggtgttcag gccgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg
     1141 cgtattaacc gttaatatca agggcgcggt attggccagc gaacaggcgc tgcgcgaagc
     1201 gggcattgaa ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc
     1261 acaatatagc attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga
     1321 agctattacc ggattcttca ctaaatatgg tgacggcggt acggacatta atccgctgta
     1381 tcgtctcaac aaacgtcagg gtaaacagtt actggcggca ttagcttgcc cggaacacct
     1441 ttataagaaa gcgccaacgg ccgatctgga agatgatcgc ccttctctgc cagatgaagt
     1501 ggcactcggc gtgacctatg acaatatcga cgactatctg gaagggaaaa acgtacctca
     1561 acaggtcgcc agaacaatag agaactggta tctgaaaacc gaacataaac gccgtccgcc
     1621 aattaccgtt ttcgatgatt tctggaaaaa gtaataattt gcacatattg gattgtgcga
     1681 aaaagagtaa tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc tataaaacta
     1741 tgcaaattca atacattgca ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt
     1801 gtaacctttg tcatcgaccc gcctcttttt taatcgcttc ccgcctgtta cactggatag
     1861 ataaccagca ttcggagtca acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg
     1921 aagctgcggc aatttatgaa tatcccgaac atttacgttc attccttaat gacttaccca
     1981 cccgaccagg ggtgtatctg tttcatggtg aaagtgacac catgccgctc tatatcggca
     2041 aaagcgttaa catccgcagc cgcgtccttt ctcatttacg taccccggat gaagccgcca
     2101 tgctacggca atcccgacgg atcagctgga tatgtaccgc cggtgaaatc ggcgctctgc
     2161 tccttgaagc gcgattaatc aaagaacaac agccgctgtt taataaacgg ttgcgccgca
     2221 atcgtcagct ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag
     2281 aggtggattt ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt
     2341 tgcaagcatt gcagaccatc gctgatgaac aaaaactttg ttatggcctg ctgggactgg
     2401 aaccgttaag tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat
     2461 gctgcggtaa agagagccat gaggaacatg cgctacgctt gcgccagtct ctggagcgtt
     2521 tgcgggtggt gtgttggcct tggcaagggg cggtggcgct gaaagaacag cacccggaaa
     2581 tgactcaata tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag
     2641 acgcgacaac gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt
     2701 gtaagccgct gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc
     2761 gagccagttg atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg
     2821 cctgccatcg aaccagcacg cgccctggtc ggtagtgagc aatttatcgc ccagttgcca
     2881 cgccccgtta atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc
     2941 ggcaattctg actttcgcct tacagacatc caggcgtgtc atgatgttga aatccatcga
     3001 catctgccct tccgtcagtt tcgcttttac cacctggtcc gctgcaaagg caaaaggctg
     3061 taacggtttt aaggtatggt taaagcggtc tgcgctttca aggagcatct cgccgccttc
     3121 cagcaacgtc actatccttt ccatgccggg aaacaaagaa aactccccat tcgccgcgat
     3181 ggatgcaatg ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc
     3241 gcgcgtttca ccggcagcat ttcgccacag attcaccgac attttacgca tatcaaagta
     3301 ttccatactc gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatggt
     3361 tatcggcaac tcgggtgatt tacgttagtg gtgatcaggc tttttttaca actgtttgag
     3421 aagagaaaag aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc
     3481 ggcggtctta agttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg
     3541 ccgctggacg ttctgtcaga cgcttctcaa aattagcatt aaattgcttt ttctgttccg
     3601 gcgtcaggat gttgtaaatt ttgttctggg tttccatgtg cgccagcatg ttagctttgc
     3661 gctgttcttc catttttgcg atctgcgctt cagcttttgc tttatcgaag gtatcgctga
     3721 caatgatgtc atgcattgcg cggcgttctt ccagcggcgg acgtttcatc tggtcacgct
     3781 ggcctttcat gatttcgcgg atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga
     3841 acatcatgtc ctgatgcgga ccgaacttgc ctttgtggtg catcatcggc ttcgcgtcag
     3901 ccggtgctgc ggcatgggcc aggttagccg cgccaagagc cagggtagag gcaacaaaca
     3961 gtgcagttaa tttacgcata ttctatatcc ttcctttcag ttatttatta cggctttctt
     4021 aagtagcgtg ccgtgttgac aagattaact ttactgactt tagcgtcaat taatcagagc
     4081 aacggtaaaa caatgaaagt gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa
     4141 aatttgatgg agagtgatga gagaatatta caacacaatg attttgcaga gattatgaag
     4201 aactatactg gatgactggt gataaataaa gcaaataacc aggattaatc tgtattaatt
     4261 tataagaaag caacttaata cccgcagaat gatttctgcg ggtaagtatt agcttatttt
     4321 ttcgagcatt aatcccgcgc gtaatcccaa cgctaccaac ggattaggga ataacacata
     4381 ctctacatca tgggttacgg taaaacgttc ctctccgtcc tgcgccagca atgttccttt
     4441 ctcaaacggc ataaaattca gcgtgtcact tgccatatgc atttcgaagg acggcgagtg
     4501 acgagtaatt tgcgaaacca cccgataacg gagcggcggt gttctcacga taccgacact
     4561 ctcaccagat agcagcgcag caattgcgct ggcagttact gcaaactggc gaagatcgtt
     4621 ttgcccaaag ggcaacgctt tgccaagttc cagcgtacag gccagcgcgc caaaatgtct
     4681 ggcgctgaaa tgggtaaacg taccaccagg ttcctgatgg aacaccagcg cctccagccc
     4741 cgccgcaccc agccacgtca gaaatttctc gtcccaggga atgtcgcgtt gcggtaatac
     4801 accgaactgc ggatgcaagg agccacgaat tgcggtatgt agatcaaggt gccagcgcac
     4861 agattctttg ccctggtcat aaaaatcttc caggcactgt tccagttcgc gcgcccgaca
     4921 ggtttctccg ctttcagcaa atagctgcca acgaccgcca aacattcgat tcatatcgct
     4981 atggcaataa cgtttccctt gcttcagcgc aggaggattc ccgaggatca ccagcaaccg
     5041 ccaacgtaac gggatctcgc cgtgagatat cgcgccaagc aacgcgtcca gcatttccac
     5101 aggtgccgtc tcattaccgt ggatccccgc agaaatcacc agcgcgcctt gcggtggcgt
     5161 taatggcgtc agttccagca caccatcgcc cagccagcgc cagcgaacgc cgttgatttc
     5221 tcgctcggtg ataaccggtt ttttacccgt taaggtcaga gcaagaaaat tatccattgc
     5281 cccctccctc gcgctggaac ggataaaccg aaccgagatt cagtaattgg ctcaatacat
     5341 ccagtgcttc ccgcccttcg cgcagcaatt gcgggtcggc cagatcggca gcagtaagac
     5401 gatcgcggta gtaacgatcc acccagtcat tgagcgcatt aaacaaggta tcgttcatca
     5461 tcaccgccgg attcaccgcc cggcgttctt cttctgtcaa taccacccgc aaccgcaggc
     5521 acgccgggcc gccgccattc gccatgcttt cacgtaagtc aaagactttt agttcgctaa
     5581 tcgggttgtc agcggcaagg agttcattga gataacccca tactccggcg tgttcccgac
     5641 actcctgagg cagcaccaac atcatggaac catcatcgcg gctcaacagt tggctgttaa
     5701 acagataggt agacaccgca tccgacacgg aaacctgagt tgccggaact tctatcgcca
     5761 taaaaccgtt cacccgcgcc cgcaggtttg ccagtaactg tgactggcga gcgaacgcct
     5821 gttggtggca aaacagcacc tggcggttac ttacggcaat cacgtcatta tgaaaaacac
     5881 cctggtcgat aacgtccggg ttttgctggg cgaaaatcac ctgttgggga ttcacctgat
     5941 taagccttgc caccgcctcg ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc
     6001 gggtatcatt gccttcttct cgcccgtaga caaaaagttg catacccggc tcaccgtaat
     6061 gaccgccgag acgattgtgg tttgccgccc cctcatcacc gagcaacgct acctgcggca
     6121 acgccgaatg gacgctaaat ttctcttcgt cgttaaaaat agcttttaac agcgattcag
     6181 tgacgggcgc ttccagcgaa cggtgaaatt tattgttcag gttggcaacg gtgagatgca
     6241 ctttgccatc cagcgtatcg gcagatggcg cgatcgttgc cgcattggct acccacattg
     6301 gcgaagcgga gctgacgctg gaaagccagt gcggtgcctg gcgtgcaact ttttccagta
     6361 cctgctcatc gctaccactg aatcccaact gacgcagcac cggaataaac ggacgctcgt
     6421 gcggcgggat cacggcctgg gggaatcctg catcggcaag ggttttcatt ttcagtaagc
     6481 cctgtttcgc cgccagtcgc gggttagaca cctgaaaacg gtgacgggta gaggcttcat
     6541 taccaaacga caggccagcg taatgatgcg tcagccctac cagcccgtcg aaattaactt
     6601 cccaggcgtt catcgcacca cctcatcgga aaaatccagc ccggggttaa gcgttgcggg
     6661 caaagttaac gagtccgact ccaggctcgc cataggccat gcgcagtaat ctgcggcata
     6721 ccaggcgctg gggcgatggt taccggatgc accaatgccg ccgaatggcg cggtactggc
     6781 agcaccagta agcggtttgt tccagttaac aatccccgcc cgcgcctcca gcaacagttg
     6841 atcgaatttt tcccgttcgg gggaaaccag accgcaagag agtccgaagc gagtgttatt
     6901 cgccattaga atcgcttcct cgaaagaatc ataacgccag acgcgcaata acggtccgaa
     6961 cacctcttca tctggtacgc cagcaacgcc tgtcatttca atgatccccg gcgtcagcaa
     7021 cgatgtctct aattgtaata agcgcggcgc aagcagggtt cgtccgccca tcgcttccag
     7081 ttgctgccag gcagtaacca cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa
     7141 cggctgcggt tcgtcatccc agttgcccgg cgttaatcgc tggctgacgg caaccagacg
     7201 agcaagaaac gcatcgccct gcgctccgct tttgagcaat aaacggcggg cgcaggtgca
     7261 gcgttgcccg gctgtgacaa acgccgactg aatggtcaga tggacagccg cgtcgatatc
     7321 cgccacctca tcgataatta gcggattatt accgcccatc tcaagggcga gaattttctc
     7381 cggctgaccg gagagctggc gatgcagctg gtagccggta ttggcgcgac cggtaaacag
     7441 caaaccgtcg agatcctcca gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac
     7501 caggttcagc acgcccggcg gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc
     7561 actccacggt gtcagttcgc tgggtttaaa gataatggtg ttacctgcca gcaatgccgg
     7621 aacgatatgt ccgttcggca aatgaccagg gaaattatac ggcccaaaca ccgccagcac
     7681 gccgtgcggg cgatgtcgca ggctcgccgc accgtccggc atttcactac gctgctcgcc
     7741 ggtacgaacg tgatacgcct taattgatat cgcgatttta ttgatcatcg ccgtcacttc
     7801 ggttgccgct tcccagcgca gcttacccgt ttctctggca ataatcgcgg ttaattcggc
     7861 tttattgctt tccagcaatc cggcaaagcg ttcgacaacg acctgacgtt cagccaatga
     7921 gagccgcgcc cagcgcggaa acgccgcacg ggctgcccga caagcctgtt cgacctgagc
     7981 ggcatcggca tcattgcctt gccataacac ctcgcccgat accggattac gcttcacacg
     8041 cgatgcgccc tggcccgtta tccagtcacc gttaatccat aaagtcatgc tgttttctcc
     8101 actgcgcaca ggcgcaccag acgaacgcga tcccctgcgt ggcatttgag ggcatccagt
     8161 tgtgcggcgg ttaaaatcaa acgctcggtt gccggatcgg tacgcgccag caccacgcgg
     8221 aaatggtgat aattttcatt ggcgaccagg caggccggaa aatcgccctg cgcaggctgc
     8281 ccttctgcca cttccaccag ccgactttta cggatggcgc gcacgcggtc gatgtcacac
     8341 tcaagcgttg gcccgccgtc gaagatgtcg atatagttgc ggtaacgaaa accttctttc
     8401 tccagcaccg cgcgggcagg cgcggtttgc ggatgtacct gaccgatgac gtcctgggct
     8461 tcctgggata aaaagtgggt atagatcgga tgtttcggca tcagttctgc aataaatgcc
     8521 ttttgcccgg tgccgcagag aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta
     8581 ccgaggcttt gccagaacgg tgaatagccg tgttcgtcaa tcaccccgcg catttcagca
     8641 accactttgt cattaaactt gtcgcgaaaa gccgccataa acataaagcg cgatttcgac
     8701 agcaaatagc cgttgccctc tttgcgccag tccgggtcga gaaacagcgt gcacagctcg
     8761 ctgctgccgg tgtgatcgtt actaagaaac agcgtcggca atgcgttata gacattcagc
     8821 tcttttgagg cgtgaaccaa cgtgccgacc cgatagttgt gccagggatc gttcagccca
     8881 accgccacct caatggcgca aatccccgcc acggtgcctg tctcgctatc ttccagcacg
     8941 aacacatagc cctgctcgct tttgggcagt tcgccttgcc aggttttgat tgccctttcg
     9001 atacgcgcgg aaagcgtggc ttcattagcg ggaagcgacg tcaggccgcc gcccgttttg
     9061 ctggcaagct gcatcagcgc cgagacatct gagcgctcaa cgggacggat gaccatcatg
     9121 atgaacctcg gctaacaaaa tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca
     9181 cctcttcttc gctgacattg agcgcaggcg caaaacgcac cacgttgcca cccgcaatca
     9241 gtaccataac gcctgctttc gccgcctcct gagagatctg tttcgcttgt ccggcgtaat
     9301 cggcattcag tacacagcca atcaacaaac ctaagccgcg aacttcactg aacaaaccgt
     9361 agcggtgatt aatggtatta agacgctcaa caaaccagtc gtgacgctgt ttaacgccat
     9421 taagcatctc tggtgtgttg atgagctcca gcactttgcc cgccaccgcc gaggccagcg
     9481 gattgccgcc ataggtggtg ccatgagtgc caacggtcat cacgctggcg cactcttcgg
     9541 ttgttaacaa cgcaccgacc gggaaatcgc cgcccagcgc tttggcggta gttaacagat
     9601 caggtgtcac gccgtagtgc atataggcat acagttcccc ggtgcgcccg acgccggttt
     9661 gtacttcatc aaaaatcaac agcgcattgt ggcggtcaca caattcacgc agaccttgta
     9721 aaaacgcgtt gctggctggc accacaccgc cttccccctg gatgggttcg acaatcaccg
     9781 cacaggtggc gtcgtcaatc agcgcgctgg cagagttaat atcgttatat gcagcatgac
     9841 ggatatctgg tggcagtggc gcaaaatcct gtgaataggc tggctgccca cccgcactga
     9901 cagtaaacag cgtgcgacca tgaaacgcat ttttaaacgc catgataccg ctcttatggc
     9961 tgccgtagcg gtcgtgagcg aatttacgcg ccagttttag cgccgcttcg ttggcttccg
    10021 caccggagtt acaaaagaag acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca
    10081 gtcgcagtac cggctcgttg gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt
    10141 cgttcagcgc ttcacgcagt tccggatgcg catggcccag cgcgttcacc gcaatgccac
    10201 ccgcgaagtc gatatactct ttcccctgct gatcccacaa gcgcgaacct tcgccacgta
    10261 ccggtataaa gggtgccgga gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg
    10321 taattggctg agacatagcg acctctacag taaataattc gttatttata tgttaacaat
    10381 aagtaatgtt tgcgctgtaa atgtagattg cagggttcgt gccagccagt gataaaagtg
    10441 cataaacggc ggaggctaac tggaaatcaa ggagttataa ccaaaccata tgcatttaaa
    10501 gtgcatataa agtgaatacg tttgcgatgt gggtgaataa aaagaataaa aaacgcaatg
    10561 ttatgcagaa gtaaaatata attctggaat tgtgatcatt gacgaaattt actggaaatt
    10621 actgcgccat tctgacgcag cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca
    10681 tcattaacaa ccatcgatca aatcacttaa caacaggcgg taagcaacgc gaaattctgc
    10741 taccatccac gcactcttta tctgaataaa tggcagcgac tatgaaattt gtctctttta
    10801 atatcaacgg cctgcgcgcc agacctcacc agcttgaagc catcgtcgaa aagcaccaac
    10861 cggatgtgat tggcctgcag gagacaaaag ttcatgacga tatgtttccg ctcgaagagg
    10921 tggcgaagct cggctacaac gtgttttatc acgggcagaa aggccattat ggcgtggcgc
    10981 tgctgaccaa agagacgccg attgccgtgc gtcgcggctt tcccggtgac gacgaagagg
    11041 cgcagcggcg gattattatg gcggaaatcc cctcactgct gggtaatgtc accgtgatca
    11101 acggttactt cccgcagggt gaaagccgcg accatccgat aaaattcccg gcaaaagcgc
    11161 agttttatca gaatctgcaa aactacctgg aaaccgaact caaacgtgat aatccggtac
    11221 tgattatggg cgatatgaat atcagcccta cagatctgga tatcggcatt ggcgaagaaa
    11281 accgtaagcg ctggctgcgt accggtaaat gctctttcct gccggaagag cgcgaatgga
    11341 tggacaggct gatgagctgg gggttggtcg ataccttccg ccatgctaat ccgcaaacag
    11401 cagatcgttt ctcatggttt gattaccgct caaaaggttt tgacgataac cgtggtctgc
    11461 gcatcgacct gctgctcgcc agccaaccgc tggcagaatg ttgcgtagaa accggcatcg
    11521 actatgaaat tcgcagcatg gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc
    11581 gccgctaatt tagcagctct cctggctcaa actgggtcag gagaattaac cttgagaaaa
    11641 atcaacaaac tgtcagtaat gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt
    11701 ttttagttgt ctgaccactt ctctattatc aagtttgata taggaaactc cacgatgaat
    11761 gctgagcgta aatttctttt cgcctgtctt atttttgcgc tggtcattta cgctatccac
    11821 gctttcggtt tattcgatct gctcaccgat ttaccccact tacagacact cattcgccag
    11881 agcggattgt tcggttatag cctctatatt ctgttattca tcattgccac cctctttctg
    11941 ttaccaggaa gcatactggt gatcgctgga ggaatagttt ttggcccgct cttagggaca
    12001 ctactctcat taattgccgc cacgctggcc tcctcgtgct cattcctgct ggcgcggtgg
    12061 ctgggacgtg atttactgct gaaatacgtg ggccatagcc ataccttcca ggccattgaa
    12121 aaaggcattg cgcgtaacgg tattgatttt cttattctga cccgcttaat cccgttgttt
    12181 ccttacaata ttcaaaatta cgcttacgga ttaaccacca tcgccttctg gccttatacc
    12241 cttatttcgg cactcacgac cctgcccggt attgttattt ataccgtgat ggcaagcgat
    12301 ctcgccaatg aaggcattac gct
//
LOCUS       DAJTNN010000123        12249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTNN010000123 DAJTNN010000000
VERSION     DAJTNN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.123"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..166)
                     /locus_tag="OO150_002961"
     CDS             complement(<1..166)
                     /locus_tag="OO150_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517034.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQE"
     gene            291..366
                     /locus_tag="OO150_002962"
     tRNA            291..366
                     /locus_tag="OO150_002962"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:324..326,aa:Ile,seq:cat)
     gene            complement(420..1184)
                     /gene="nfeF"
                     /locus_tag="OO150_002963"
     CDS             complement(420..1184)
                     /gene="nfeF"
                     /locus_tag="OO150_002963"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR5517035.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1472..2095
                     /gene="nfeR"
                     /locus_tag="OO150_002964"
                     /gene_synonym="yqjI"
     CDS             1472..2095
                     /gene="nfeR"
                     /locus_tag="OO150_002964"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5517036.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2249..3769)
                     /gene="aer"
                     /locus_tag="OO150_002965"
     CDS             complement(2249..3769)
                     /gene="aer"
                     /locus_tag="OO150_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR5517037.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSIIASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDVSADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4186..5565
                     /gene="ygjG"
                     /locus_tag="OO150_002966"
     CDS             4186..5565
                     /gene="ygjG"
                     /locus_tag="OO150_002966"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5517038.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLLAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQSELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5607..5939)
                     /locus_tag="OO150_002967"
     CDS             complement(5607..5939)
                     /locus_tag="OO150_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR5517039.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6158..7141
                     /gene="ebgR"
                     /locus_tag="OO150_002968"
     CDS             6158..7141
                     /gene="ebgR"
                     /locus_tag="OO150_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5517040.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7325..10417
                     /gene="ebgA"
                     /locus_tag="OO150_002969"
     CDS             7325..10417
                     /gene="ebgA"
                     /locus_tag="OO150_002969"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5517041.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLGNLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            10414..10863
                     /locus_tag="OO150_002970"
     CDS             10414..10863
                     /locus_tag="OO150_002970"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5517042.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            10926..>12249
                     /locus_tag="OO150_002971"
     CDS             10926..>12249
                     /locus_tag="OO150_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5517043.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVG"
BASE COUNT         2946 a         3300 c         3088 g         2915 t
ORIGIN      
        1 cctcctgcgg cttcaactga cggtcggtaa acccggcctg atatatcacc ttccagaagc
       61 gatttgctgg atgagcaaag ggaaaaccag tcccggcgga tgaaagccca gggttgatac
      121 cgcaaaacac gacccgtaac cctggagcca aaatatcctc aaccatattt actcctttct
      181 aacatcacca ggaaaagtat aacggattga aaatacattg tttataaaaa cagcaggcgc
      241 acggtaatgg ctggattgcg acacggagtt actttataat ccgctaccat ggccccttag
      301 ctcagtggtt agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcagggg
      361 ccaccagata tagcaaaggc tgacgagaaa tcgtcagcct ttttcttttt atatctcagt
      421 tactttgcgt gccagtaagc cgctgcacgt acccgctgtg ggtcatactg ttccgcttca
      481 aagcggcggc ttaaattctt aacgacttta ccttcgccgg ttatccagat gaagtaatca
      541 tcggcaggga tttgcatctg cgccagacgc gcatctaccg cctgctcatc atgtgccagc
      601 cattcgatat taaaaccatc aaggtgcgcg agataatcct gacaggcgtt atcccgcacg
      661 ctaaccagcg cactaacttg cggtttaacg gcaagtttgc tcaacgtttc caggcggcgg
      721 cgcagtgcgg gcattccgga ttcatcgcag acatacagct gatacgcgta atcttccggc
      781 accaccagcg aaccgcgcgg accagccacc gtaagtttat cgcccggttg cgcctgcatc
      841 gcccagccgc tggcgacccc accgtcgtga ataaagaaat cgatcgccag ttcatggcgt
      901 agttcgtcat acagcggcgt atagtcacgc gacggtgggc gtggtccttc cggccagacg
      961 atgccctctt ccgttaccgt tggcggcaca aagtgagcgt caggttgagg aaagaagagt
     1021 ttgctgtgat cgtcaaagcc gcgcgatgta aaaccgtcca gcgcctcgcc gccgaggaca
     1081 atgcgctgaa aaccggcgct gatgcgctca acgcgtaaca cagtcagttc acggaagcgc
     1141 agatcattgc gaacgcgctg cgggtagcgg ggggagttat tcattgttat cgccttcgtg
     1201 atggtaatca gatatatcta aataattttc gtaaatgata atgattgtta atcatgataa
     1261 atgcaagcga ttagtagaac tgatatgtct atagtctgat aagacgaacc gcctcttctc
     1321 aggcatcatt actcaacgcc ggatgcggcg tgaacgcctt atccggccta cgtgtgagat
     1381 gagtccacat ttaaaaatca tttttattct tgcaagagtg ctcatatcag atataaatta
     1441 gatatatcta atcaagaaaa aggaggctga tatgagccat catcacgaag ggtgttgtaa
     1501 acatgaaggc cagccacgcc atgagggctg ctgcaaaggt gagaagtcag aacacgagca
     1561 ctgcggacac ggtcaccagc atgaacacgg tcaatgctgc ggtggtcgcc acggtcgcgg
     1621 cggcggtcgt cggcaacgtt tctttggtca cggtgaattg cgtctggtga ttctggatat
     1681 tctctcgcgc gatgacagcc acggttacga attgattaaa gcgattgaga atctaaccca
     1741 ggggaattac accccaagcc cgggcgtcat ctacccaacg ctggattttc tgcaggagca
     1801 gtcgctgatt accatccgcg aagaggaagg tggtaagaag cagattgcgc tgaccgaaca
     1861 aggcgcgcag tggctggaag aaaaccgcga acaggtggag atgattgaag aacgcatcaa
     1921 agcgcgttgc gttggcgcgg cgctgcgcca gaacccgcaa atgaagcggg cgctggataa
     1981 ttttaaagcg gtgctggatt tacgcgtcaa ccagagcgat atcagtgatg cacaaataaa
     2041 aaagatcatt gcggtgatcg accgcgccgc ttttgatatt acgcaactgg attaatcgcc
     2101 gcatccgcca gtggcgcggt gcaattgccg gatgcgacgc ttgacgcgcc ttatccggcc
     2161 tacacccact acacaccccg caggcctgat aagatgcgcc agcatcgcat caggcattgt
     2221 gctccaaccg ccggatccgg cataccgatt aatgcagcac cgtcaccgcg tcttccagtc
     2281 ggctggcgcg gtgtttcacc atcgccgaca cctgcgcact ctcttccacc agctcagcat
     2341 ttttctgggt gatcaggtta agctcatcca ctgcacgggt caggctggaa agcccatcgg
     2401 cctgttccag cgttgaatgg ctaatctggg cgatcaactg ggtgacgttt ttcacctgtg
     2461 ccacaatatc ttccatcgtc cgtccggcgg cgtgtacctg ctgcgaaccg gattgcacct
     2521 tatcagcact gacatcaatc agcttgcgaa tatcgttggc agcattagcg ctgcggctgg
     2581 ctaaatgacg cacttcccct gccaccactg caaaaccttt gccctgttcg ccggcacgcg
     2641 ccgcttccac cgccgcattc agggccagaa tattggtctg aaacgcaata tcgttaatca
     2701 gcgaagtaat ggtgccaatg cgctgggtac tgtcggcgat atcgtccatt gtcttgatca
     2761 ccgtggtcat cgcctcccca ccctgcactg ccgcattact ggcgatgata gaaagtttat
     2821 cggcagccga cgccgtggca gagttctgtt tcaccgacgc cgccatttgg ttcatggtcg
     2881 ccaccgtttg ctgaacgtta tcaactgtct gctgggtatg ttcgttcagt tcatcggtgc
     2941 ctttcgccag cgtctcactg ccatttctga cactggacac ctggcttgag acatcgttaa
     3001 ttagccaacg gcacatcagg ccaagctgcc ctaccgcgcg taatgtcagc cccagctcat
     3061 cgctgcgatt caggtgctca acactattac gctctccggt cgccaccttc agtgcctgac
     3121 gggcgacatt ttctatcggg cgcacaatct gccattcaaa acaagcgctt gccaacaata
     3181 ccactaacac acagaggaaa tacgtcacca ccggggcagc aacaaaccaa agcatggccg
     3241 ccagcaagat aaacatcagg gtcatcactc cacgcgcccg ccagcgaagc ggtaatgaag
     3301 gcagtttacc cagccagcct ttacgcacca ccaggccttt atgaatacgc ttactggtac
     3361 gtccggcgtt cagcgctttg tacagcggct ccaccgccgc aatctcttca tccgtcgccc
     3421 gggtacgaat cgacatatag ccactgattt ttccctcgcg caccatcggt accgcattgg
     3481 cccgcaccca ataatggtcg ccatttttgc ggcgattttt cacgatgccg ctccagggct
     3541 cccctttttt cagggtgaac cacatatccg caaacgccgc ttttggcata tccgggtgac
     3601 gcaccatgtt gtgcggctgc ccttgtaact cttgcaaggt aaagccgctc acctgcacaa
     3661 aagtgtcatt agcatgagtg atatagcttt gcagatcggt agtggacatc agagtggtat
     3721 cgtccgccag cggggtattt tgctgggtga catacggatg agaagacatg ttcgcgtcct
     3781 atgcaggtta tatggttgtt aacttcttgt cagagtttat gtcggccccg ctgcggttat
     3841 ctttaaccga ttaatttgat ttagatcgca atttgcgatt aaagcacaaa tctaattcct
     3901 tgatttaaaa tactttcact ctgttactat acgaaaacgt taattatctt gcccaaaatc
     3961 aggcaattat cgccctgaaa acgtgcattt gcacagcaat catcaaatcc atacccgaca
     4021 aaaaccgtgc aaaataacaa cgaatgttaa caaatagcat taaatactgc gcaaatgata
     4081 accacatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca atccctgcaa
     4141 tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa caggttacct
     4201 tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga gaagcgaacg
     4261 ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt caaagagcat
     4321 gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga ttacggagcc
     4381 gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca ggagtttatc
     4441 gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt tgtggtttcc
     4501 gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct cgatccgtta
     4561 cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa atacagcttc
     4621 ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa agcttaccag
     4681 tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg taaatcactt
     4741 ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc gttactgccg
     4801 ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct taacgagtgc
     4861 aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg tgaaggtggc
     4921 gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga tgagttcggc
     4981 gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa gatgttcgcc
     5041 tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct cggcggcggc
     5101 gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct gttcgacaac
     5161 ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc ggcgctggcg
     5221 accatcaatg tgttgctgga gcagaactta ctggctcagg ctgagcaaaa aggcgatatg
     5281 ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca ggaagcgcgt
     5341 ggtaaaggga tgttgatggc aattgagttt gttgataacg aaatcggcta taactttgcc
     5401 agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc caaaacgatc
     5461 cgcattgaac cgccactgac actgaccatt gaacagtctg aactggtgat caaagcggcg
     5521 cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca aatcggatgg
     5581 cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg catcatccgc
     5641 tccggcgtta acaacacgct ttcactgccg tcatcggttt ccgcacacaa cagcatgcat
     5701 tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac caccgttttc
     5761 cccatcagct cttcttcgct gtagtacggc accaggctgg tcacggtttg cagtgttttt
     5821 tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg tttcacttcc
     5881 acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac ggtttccatt
     5941 gtttccttcc ttcggataaa attttactaa actatagcaa aagtttttct caatcctgta
     6001 ggctaaaaat ggagaatgca ggcgtgatca cattcgtgag ccgctgtgtt accgttacag
     6061 cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagtac ttcctctaca
     6121 atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacactaa aagacatcgc
     6181 aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg atccgacatt
     6241 gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc tggagtacaa
     6301 gaccagtagt gcccgcaaac tccagacagg tgcagtcaac caacaccata ttctggctat
     6361 ctacagctac caacaggagc tggagatcaa cgatccttac tatctggcga tccgccacgg
     6421 cattgaaact cagtgcgaaa agctgggcat tgagctcacc aactgttatg aacacagcgg
     6481 attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca cgcccgccct
     6541 tcgcgccgcc gcctgtgcgt tgaccgacaa tatctgtttt atcgactttc acgaacccgg
     6601 cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa tcatcgactt
     6661 ctatatcaat cagggcgtta atcgtattgg ttttattggc ggtgaagatg agccaggcaa
     6721 agcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag tggtacgcga
     6781 agaggatatc tggcgcggag gcttttccag ttcgtcgggt tatgaactgg caaaacagat
     6841 gctggcgcag gaagactatc cgaaggcact gtttgttgct tccgattcca ttgctatcgg
     6901 cgtactgcgg gcaattcatg aacgaggcct gaacatccca caggatattt cgcttatcag
     6961 cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg tgcgcatcca
     7021 ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc gcgatggtcg
     7081 cgcgctgccg ctgttagtct tcgttcccag caaattaaaa ctgcgcggca cgacccgtta
     7141 aatcccctta cacactgtcc ggcaatcgtt tttgccggac agtgctgccg tttattttcg
     7201 tgatccagtt aaagtaaatg catttatctg ctacttttta gtaaaaattt tactaaactc
     7261 cccagcaatt acacaaacta ccatcaccat gaatggttcc gatttctctc taccgggagg
     7321 ccctatgaat cgctgggaaa acattcagct cacccacgaa aaccgacttg cgccgcgtgc
     7381 gtactttttt tcatatgatt ctgttgcgca agcgcgtacc tttgcccgcg aaaccagcag
     7441 cctgtttctg cccttaagcg gtcagtggaa tttccacttt tttgaccatc cgctgcaagt
     7501 accagaagcc ttcacctctg agttaatggc tgactggggg catattaccg tccccgccat
     7561 gtggcaaatg gaaggtcacg gcaaactgca atataccgac gaaggttttc cgttccccat
     7621 cgatgtgccg tttgtcccca gcgataaccc aaccggtgcc tatcaacgta ttttcaccct
     7681 cagcgacggc tggcagggta aacagacgct gattaaattt gacggcgtcg aaacctattt
     7741 tgaagtctat gttaacggtc agtatgtggg tttcagcaag ggcagtcgcc tgaccgcaga
     7801 gtttgacatc agcgcgatgg ttaaaaccgg cgacaacctg ttgtgtgtgc gcgtgatgca
     7861 gtgggcggac tctacctacg tggaagacca ggatatgtgg tggtcagcgg ggatcttccg
     7921 cgatgtttat ctggtcggaa aacacctaac gcatattaac gatttcactg tgcgtaccga
     7981 ctttgacgaa gcctattgcg atgccacgct ttcctgcgaa gtggtgctgg gaaatctcgc
     8041 cgcctcccct gtcgtcacga cgctggaata taccctgttt gatggcgaac gcgtggtgca
     8101 cagcagcgcc attgatcatt tggcaattga aaaactgacc agcgccagct ttgcttttac
     8161 tgtcgaacag ccgcagcaat ggtcagcaga atccccttat ctttaccatc tggtcatgac
     8221 gctgaaagac gccaacggca acgttctgga agtggtgcca caacgcgttg gcttccgtga
     8281 tatcaaagtg cgcgacggtc tgttctggat caataaccgt tatgtgatgc tgcacggcgt
     8341 caaccgtcac gacaacgatc atcgcaaagg ccgcgccgtt ggaatggatc gcgtcgagaa
     8401 agatctccag ttgatgaagc agcacaatat caactccgtg cgtaccgctc actacccgaa
     8461 cgatccgcgt ttttacgaac tgtgtgatat ctacggcctg tttgtgatgg cggaaaccga
     8521 cgtcgaatcg cacggctttg ctaatgtcgg cgatatcagc cgtattaccg acgatccgca
     8581 gtgggaaaaa gtctacgtcg agcgcattgt tcgccatatc cacgcgcaga aaaaccatcc
     8641 gtcgatcatc atctggtcgc tgggcaatga atccggctat ggctgtaaca tccgcgcgat
     8701 gtaccatgcg gcgaaagcgc tggatgacac gcgactggtg cattacgaag aagatcgcga
     8761 tgctgaagtg gtcgatatta tttccaccat gtacacccgc gtgccgctga tgaatgagtt
     8821 tggtgaatac ccgcatccga agccgcgcat catctgtgaa tatgctcatg cgatggggaa
     8881 cggaccgggc gggctgacgg agtaccagaa cgtcttctat aagcacgatt gcattcaggg
     8941 tcattatgtc tgggagtggt gcgaccacgg gatccaggca caggacgacc acggcaatgt
     9001 ctggtataaa ttcggcggcg actacggcga ctatcccaac aactataact tctgtcttga
     9061 tggtttgatc tattccgatc agacgccggg accgggcctg aaagagtaca aacaggttat
     9121 cgcgccggta aaaatccacg cgcgggatct gactcgcggc gagttgaaag tcgaaaataa
     9181 actgtggttt accacgcttg atgactacac cctgcacgca gaggtgcgcg ccgaaggtga
     9241 aacgctcgcg acgcagcaga ttaaactgcg cgacgttgcg ccgaacagcg aagccccctt
     9301 gcagatcacg ctgccgcagc tggacgcccg cgaagcgttc ctcaacatta cggtgaccaa
     9361 agattcccgc acccgctaca gcgaagccgg gcattctatc gccacttatc agttcccgct
     9421 gaaggaaaac accgcgcagc cagtgccttt cgcaccaaat aatgcgcgtc cgctgacgct
     9481 ggaagacgat cgtttgagct gcaccgttcg cggctacaac ttcgcgatca ccttctcaaa
     9541 aatgagtggc aaaccgacat cctggcaggt aaatggcgag tcgctgctga cccgcgagcc
     9601 aaagatcaac ttcttcaagc cgatgatcga caaccacaag caggagtacg aagggttgtg
     9661 gcagccgaat catttgcaga tcatgcagga acatctgcgc gactttgccg tagaacagag
     9721 cgatggtgaa gtgttgatca tcagccgcac agttattgca ccgccggtgt ttgacttcgg
     9781 gatgcgctgt acctacatct ggcgcatcgc tgccgatggc caggttaacg tggcgctttc
     9841 cggcgagcgt tacggcgact atccgcacat cattccgtgc atcggtttca ccatggggat
     9901 taacggcgaa tacgatcagg tggcgtatta cggtcgtgga ccgggcgaaa actacgccga
     9961 cagccagcag gctaacatca tcgatatctg gcgcagcacc gtcgatgcca tgttcgagaa
    10021 ctatcccttc ccgcagaaca acggcaaccg tcagcatgtc cgctggacgg cactgactaa
    10081 ccgccacggc aacggtctgc tggtggttcc gcagcgccca attaacttca gcgcctggca
    10141 ctatacccag gaaaacatcc acgctgccca gcactgtaac gagctgcagc gcagtgatga
    10201 catcaccctg aacctcgacc accagctact tggcctcggc tccaactcct ggggcagcga
    10261 ggtgctggac tcctggcgcg tctggttccg tgacttcagc tacggcttta cgttgctgcc
    10321 ggtttctggc ggagaagcta ccgcgcaaag tctggcgtcg tatgagttcg gcgcagggtt
    10381 cttttccacg aatttgcaca gcgagaataa gcaatgagga tcatcgataa cttagaacag
    10441 ttccgccaga tttacgcctc tagcaagaag tggcaacgct gcgttgaagc gattgaaaat
    10501 atcgacaaca ttcagcctgg cgtcgcccac tccatcggtg actcattgac ttaccgcgtg
    10561 gagacagact ccgcgaccga tgcgctattt accgggcatc gacgctattt tgaagtgcat
    10621 tactacctgc aagggcagca aaaaattgaa tatgcgccga aagagacatt acaggtagtg
    10681 gaatattatc gtgatgaaac tgaccgtgaa tatttaaaag gctgcggaga aaccgttgag
    10741 gtccatgaag ggcaaatcgt tatttgcgat atccatgaag cgtatcggtt tatctgcaat
    10801 aacgcggtca aaaaagtggt tctcaaagtc accatcgaag atggttattt ccataacaaa
    10861 taacaactac ggcggcaaaa ggagtttgcc gccaccgcta ccctacacat tttcggagat
    10921 gtgttatgtc tgataccaaa cgtaatacaa tcggcaaatt cggcttgctc tcgctgactt
    10981 ttgctgccgt ttacagcttt aacaacgtta tcaataataa tattgagctt ggactggcct
    11041 cggcaccgat gtttttcctc gcgacgattt tttattttat tcccttctgt ctgatcatcg
    11101 cagaatttgt ttcgttaaat aaaaactcag aagccggtgt ctacgcgtgg gtaaaaagtt
    11161 cgctgggcgg acgttgggca tttattactg cctataccta ctggttcgta aacctgttct
    11221 ttttcacctc actgttgccg cgcgttattg cttatgcttc gtatgccttc ctcggatacg
    11281 aatatattat gacgccggtt gccaccacca ttatcagtat ggtgctgttc gccttctcca
    11341 cctgggtttc caccaacggg gcgaaaatgt tggggccaat tacctccgtc acttcaacgc
    11401 tgatgctgct gttaacgctc tcctacattt tactggcagg tacggcgctg gttggcggcg
    11461 tacagcctgc tgaccccatc accgttgacg cgatgatccc gaacttcaac tgggcgttcc
    11521 tcggcgttac cacctggatc tttatggccg caggtggcgc ggagtccgtc gctgtgtacg
    11581 ttaacgacgt caaaggcggt tcgaaatcgt tcgttaaagt gatcatcctc gccgggattt
    11641 ttatcggcgt actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag
    11701 agttgaaatt taccggcgga tcggtgcagg tattccatgg catggcggcg tattttggtc
    11761 taccggaagc gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt
    11821 ccctgctgat gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca
    11881 tctttggtaa gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga
    11941 tccagttcct gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc
    12001 aggatctgat gaatacaatt attaatatga ccgccgcagc gtccatgctt ccgccgttat
    12061 tcatcatgct ggcttacctg aatttacgcg ccaaattaga tcacctgcca cgcgatttcc
    12121 gtatgggctc ccgccgcacc ggtattatcg ttgtttcaat gctgattgcg atatttgccg
    12181 tagggtttgt cgcttcgaca ttcccgactg gcgcgaatat tctgaccatc attttttata
    12241 acgtcggcg
//
LOCUS       DAJTNN010000124        12216 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTNN010000124 DAJTNN010000000
VERSION     DAJTNN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.124"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..682)
                     /gene="ychF"
                     /locus_tag="OO150_002972"
     CDS             complement(<1..682)
                     /gene="ychF"
                     /locus_tag="OO150_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5517044.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAA"
     gene            complement(799..1383)
                     /gene="pth"
                     /locus_tag="OO150_002973"
     CDS             complement(799..1383)
                     /gene="pth"
                     /locus_tag="OO150_002973"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5517045.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            1661..1939
                     /gene="ychH"
                     /locus_tag="OO150_002974"
     CDS             1661..1939
                     /gene="ychH"
                     /locus_tag="OO150_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5517046.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(1994..3673)
                     /gene="dauA"
                     /locus_tag="OO150_002975"
     CDS             complement(1994..3673)
                     /gene="dauA"
                     /locus_tag="OO150_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5517047.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVIIYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPISGRLAFFPNRRAAMADL"
     gene            complement(3798..4745)
                     /gene="prs"
                     /locus_tag="OO150_002976"
     CDS             complement(3798..4745)
                     /gene="prs"
                     /locus_tag="OO150_002976"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5517048.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(4896..5747)
                     /gene="ispE"
                     /locus_tag="OO150_002977"
     CDS             complement(4896..5747)
                     /gene="ispE"
                     /locus_tag="OO150_002977"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5517049.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(5747..6370)
                     /gene="lolB"
                     /locus_tag="OO150_002978"
     CDS             complement(5747..6370)
                     /gene="lolB"
                     /locus_tag="OO150_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5517050.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            6584..7840
                     /gene="hemA"
                     /locus_tag="OO150_002979"
     CDS             6584..7840
                     /gene="hemA"
                     /locus_tag="OO150_002979"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5517051.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7882..8964
                     /gene="prfA"
                     /locus_tag="OO150_002980"
     CDS             7882..8964
                     /gene="prfA"
                     /locus_tag="OO150_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5517052.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            8964..9797
                     /gene="prmC"
                     /locus_tag="OO150_002981"
     CDS             8964..9797
                     /gene="prmC"
                     /locus_tag="OO150_002981"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5517053.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            9794..10186
                     /locus_tag="OO150_002982"
     CDS             9794..10186
                     /locus_tag="OO150_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5517054.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10190..10999
                     /locus_tag="OO150_002983"
     CDS             10190..10999
                     /locus_tag="OO150_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5517055.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11035..11889
                     /gene="kdsA"
                     /locus_tag="OO150_002984"
     CDS             11035..11889
                     /gene="kdsA"
                     /locus_tag="OO150_002984"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5517056.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12038..12145)
                     /locus_tag="OO150_002985"
     CDS             complement(12038..12145)
                     /locus_tag="OO150_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5517057.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3035 a         3100 c         3202 g         2879 t
ORIGIN      
        1 tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt ttcaaaaccg tcttcgttga
       61 cgttggcgat gtacattgtt ggtttcagcg tcaggaagct caggtaacga atagccgctt
      121 tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc attttccaac tggggcaggc
      181 atttttccag gaccgccagc tcagctttcg cgtctttatc gccacctttg gctttcttct
      241 gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc cagcgccagt tcggtgttga
      301 taacttcaat atcgtcagcc gggttaactt tgccggaaac gtgaatgatg ttgtcatttt
      361 caaagcagcg aacaacgtga ccgatcgctt cggtttcacg gatgttggtc aggaactggt
      421 tacccagacc ttcgcctttc gatgcgcctt ttaccagacc ggcgatatcg acaaattcca
      481 tggtcgtggg aagcgtacgc tgcggtttta cgatttcagc cagttgatcc aggcgaggat
      541 caggcattgg tacgacgcct gtgttcggct caatggtgca gaatggaaag ttggccgctt
      601 caataccggc tttggtcagc gcgttgaaca gggtagattt cccgacgttg ggcaaaccga
      661 cgataccgca tttgaatccc atgatttaaa tcaccttaat atcttaataa tcaacctgtt
      721 attgctaaca gattgcagaa atggaaataa ctttgcctat tatacacggc actcggcaaa
      781 aatgccgcag acaacgactt attgcgcttt aaaggcgtgc aatcggttcg ttgctttggt
      841 caagccatct gtaaaccaca tttcagtaca acgcgccgct tcgtcaatgg cttcatcaat
      901 taacttctgt tcactaacag gcggtttgcc taacacaaaa ccgacaactt tatttttatc
      961 gcccggatga ccgattccga tgcgtaaacg gtgaaagtta gggttattac ccaatttact
     1021 gatgatgtct ttcagtccat tgtgaccacc atggccaccg cccaatttaa atttggcgac
     1081 gccaggaggc agatccagtt cgtcgtgggc caccagaatt tcgtccggat taatgcggaa
     1141 aaaactggcc atcgccgcaa cggctttgcc gctgagattc ataaatgtag tcgggactaa
     1201 caggcggaca tcttcgcctc caagagtgac tcgcgaagta taaccaaaga atttagcctc
     1261 ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca acgaaccagg caccagcatt
     1321 atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg ccgacaatca atttaatcgt
     1381 cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta gtttactggt tgcgaccccg
     1441 cttgacaaaa aactgcgtat caaatgcaga taacgtaata attgcctgag tgggctatta
     1501 gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt tgaaataagg gttgtaattg
     1561 tgatcacgcc cgcacataac ccacagggtg ttgtctatac tttacacata aggaagaggg
     1621 gtattccctg ttacaaccca gaaagttccg gaggtgacat atgaaacgca aaaacgcttc
     1681 gttactcggt aacgtgctca tggggttggg tctggtggta atggtggtcg gcgtggggta
     1741 ttcaatcctc aaccagttac cacagtttaa tatgccccag tatttcgcac atggtgcagt
     1801 gctaagtatt ttcgtcggtg ccattctctg gctggcgggt gcccgtgttg gcgggcatga
     1861 acaggtgtgc gaccgttact ggtgggttcg ccactatgac aaacgttgcc gccgtagcga
     1921 taatcgccgt catagctaac aaataatgca gtttgctgac cagtcagttt gcgctgactg
     1981 gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg attcgggaag aacgcgaggc
     2041 gtcccgagat tggttgaatg cctgcgcgag ccatagtgcg cagtggctgg aattccacgt
     2101 tgcacacgcg cagttcacat ccttcgggca gacgcttcac aaaacgctgg aacgcatcaa
     2161 gaccaccagc atcaagtacc ggaacggcat cccacttcag aatcacaatc cgtttgcctt
     2221 caagacgtga ctccaggtcc gtgaacaagc cttcagcagc agcaaaaaac agcgggccaa
     2281 taacgcgcag taccagaaca tcgtctggaa catctacgac taccggtgcc aggcgagtca
     2341 tacgtgcgat acgacgcata aacagcagcg atgccagcac gatcccgacg ctgatggcaa
     2401 taaccatatc aaacagcacg gtcagcgaca tgcacagcag catgacaatg atgtcatctt
     2461 tcggtgcatg acgcagcaag tcgaccactt tatgcgcttc actcatgttc cacgccacca
     2521 tcaacagcag ggctgccata gcggaaagcg gcagccagga gagcagcggt gccagtacca
     2581 gcagggcaag aataaccaga atagagtgga tcaccgctga gataggggaa gttgccccgg
     2641 cacggacgtt agcggcagaa cgcgcgatgg cagctgtagc ggtaatacca ccaaagaatg
     2701 gagcgataat attccccagt ccctgtccaa ccagttcgct gttcgccttg tgtttcgtcc
     2761 cggtcatacc atccagtacc acggcgcaga gcagagattc gattgcgccg agcattgcca
     2821 ttgagaatgc cgcaggcagc agtgtgcgaa tagaatccca ggttagcgtg aattctgaat
     2881 taggcagatc ccacggcagc accagttgcg gcagcagttg cggaataccg ttaccctgag
     2941 aaccatcggc caggacgtag tggaattgcg aaccgatggt agcaacatgt ccgccgagca
     3001 ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc cggaaggtga ccgggtaaac
     3061 gaatgcccag acgcggccaa aaaacaagaa tacctagcgt cacaatgcca atggcagcat
     3121 cacccacatt aatggtcggc agcgccataa ataatgcgcc gactttttgt agataatgtt
     3181 ccgggacatg ggccatttgc agaccgagaa aatctttaat ctgcatggta ccgatggtga
     3241 tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg aatatactca atcaggcgac
     3301 caaagcgtgc cagacccatc agaatcaaaa agatccccga cagcaaggcc gcaaccagca
     3361 gtcctgccag tccaaactgt tgcgacacgg gatagataat taccacaaat gccgcagtcg
     3421 gaccggaaac gctaaagcgt gacccacccg tcagagcaat gacaatcccc gcaacagctg
     3481 cggtatataa accgtactgg ggtgccacac cactaccaat agccaacgcc atcgccagcg
     3541 ggatagcaat aatcccgacg gttatcccgg caatcaggtc acgggtaaac cgtgcggcag
     3601 tatatttttc tttccagcaa gcgtcgatca gagcgcggaa aggcatcaca tgtgaggaaa
     3661 atattttgtt cacaataatg tttcatccgt gagcgcatca tctgtcaact aaatggcagg
     3721 tgaaggaggc ataggtcata caaatggata ttacagacaa aaaaacccgc cgcagcgggt
     3781 ctttgagccg ggttcgatta gtgttcgaac atggcagaga tcgattcttc gttgctgata
     3841 cgacgaatcg cttcggccag catacctgac agggtcagag tacgcacgtt cggcagtgat
     3901 ttgatttcat cgctcagcgg aatggtatcg cagacaacga cttcatcaat tacagagtta
     3961 cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag tcgcgtacgc aaatacacgt
     4021 ttagcaccac gttctttcag agcttcagca gctttacaca gcgtaccgcc agtgtcgatc
     4081 atatcatcga ccagtacgca gtcacgacct gcaacgtcac cgatgatatg catcacctgg
     4141 gaaacgttcg cacgcggacg acgtttgtcg atgattgcca tatcggtatc gttcagcagc
     4201 ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg gagaaaccac aattgggtta
     4261 tccagattca gctgcagcat gtcttccagc aggatcgggc taccaaatac gttatcaacc
     4321 ggaacgtcga agaaaccctg aatctgttca gcgtgcagat ccactgtcag cacacggtca
     4381 acaccgacgc tggagaggaa gtctgcaacc actttcgcag tgattggtac acgagcggaa
     4441 cggacgcgac ggtcctggcg cgcatagcca aagtagggga taacagcggt gatacgacct
     4501 gcggaagcac gacgcagggc atcaaccata acgactaatt ccatcaggtt gtcgttagta
     4561 ggggcacaag tggactggat gatgaaaata tcaccaccgc gtacattttc attaatttgt
     4621 acgctgactt cgccatcgct aaagcgacct acagcggcgt cgccgagtga agtgtacagg
     4681 cggttggcaa tacgttgtgc tagttccggg gtggcgttac cagcaaaaag cttcatatca
     4741 ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa gaatctgccg aaaactgtgc
     4801 gggccaggca tgatcctttc caggcagtgt attaaagagc gcgatgcaac gtctggaaca
     4861 aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa gcatggctct gtgcaatggg
     4921 gaaagattaa cgcctttcgc cacaaagcca ttgagccatt ccggggcttg ctctagcacc
     4981 tggcgggctt cagactctgt atcaaattca gcaaagacac aggcccctgt cccagtcagg
     5041 cgcgacgggg cgtattctaa cagccaggaa agcaccgcat caacctcgcg aaaacgtttt
     5101 cttgcgataa cctcgcaatc attgctgaat tcacatttta gcaacgtttc tattgacctt
     5161 tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca ccggagtcgg aatacttaca
     5221 ccagggtgcg ccaccagata ccacttctct ggcggatcca ccggcgttag tatttcacca
     5281 acgccttcgg caaacgcggc atgcccccga acaaagacag gaacatctgc gcccagcgtc
     5341 agccccattt ccgccagctc atccatgctt agcccgcatt gccagagatg atttaatgcc
     5401 accaggaccg tcgcggcatt ggatgaacca ccgccgagac cgccgcccat cggcaaacgc
     5461 ttgtcaatgc tgatattcgc accgcttccc gtcggaagac gcccgctgtc tgccgcagtt
     5521 ttcatcaaca atcgcgctgc gcgaacgatc aggttatctt catgttccac gccttcaacg
     5581 ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa tgctgatggt gtcgccgtaa
     5641 tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat ccgcacgctg accggtaatg
     5701 tataaaaaca gattaagttt tgccggagag ggccactgtg tccgcattat ttcactatcc
     5761 agttatccat ttttaacttg atgcgttgac caccgtcggt gagttccata ttggctggca
     5821 tcgcaggatg cgttttggtg tcataaccac cataaacaac cttccagttt ttgccattct
     5881 ggctgtaggt aatttcgctc aggcggtact ggtcgtccag tttgtagtcg gttgcatcac
     5941 ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg aattggcatt ccggtcaatt
     6001 tgccaatcat ctcttcggcg tcatcggcgg tataacgctg acctttattg tcgactaact
     6061 gcacgttacc cggttgagca ttcagctcca gttccgtgct gcccaatggg ttagtgagca
     6121 gcagacggta gcgatcctgg ccggtttgct gccagaaaaa gcgggcgtac actttttgtt
     6181 ggtcagaaat ataagcgaac gcgccgcgag tctgatactg attaagattg cgcacgtctt
     6241 gctgatgctg acgccattgt ggcgaatccg ggcttttgcc aggacctttg ggcgtggtaa
     6301 cggaacaggc agtgagcaca agagcagcca gcggtagcag gcggataaga cgaaaatcgg
     6361 gcaggggcat agtgatgaca agtccttgag atacgttgca gttataaccc ttaatgctag
     6421 cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat tgccagaatc taacggcttt
     6481 cggcaattac tccaaaaggg ggcgctctct tttattgatc ttacgcatcc tgtatgatgc
     6541 aagcagacta actctatcaa cgttggtatt atttcccgca gacatgaccc ttttagcact
     6601 cggtatcaac cataaaacgg cacctgtatc gctgcgagaa cgtgtatcgt tttcgccgga
     6661 taagctcgat caggcgcttg acagcctgct tgcgcagccg atggtgcagg gcggcgtggt
     6721 gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt gaagagcagg ataacctgca
     6781 agaggcgtta atccgctggc tttgcgatta tcacaatctt aatgaagaag atctgcgtaa
     6841 aagcctctac tggcatcagg ataacgacgc ggttagccat ttaatgcgtg ttgccagcgg
     6901 cctggattca ctggttcttg gggagccgca gatcctcggt caggttaaaa aagcgtttgc
     6961 cgattcgcaa aaaggtcata tgaaggccag cgaactggaa cgcatgttcc agaaatcttt
     7021 ctctgtagcg aaacgcgttc gcactgaaac agatatcggt gccagtgctg tgtctgtcgc
     7081 ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg ctctctacgg tcacagtgtt
     7141 gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt catctgcgcg aacataaagt
     7201 acagaagatg attatcgcca accgcactcg cgaacgtgcc caaattctgg cagatgaagt
     7261 tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt ctgcgcgaag ccgatatcat
     7321 catcagttcc accgccagcc cgttaccgat tatcgggaaa ggcatggtgg agcgcgcatt
     7381 aaaaagtcgt cgtaaccaac caatgctgtt ggtggatatt gccgttccgc gcgatgttga
     7441 gccggaagtt ggcaaactgg cgaatgctta tctttatagt gtggacgatc tgcaaagcat
     7501 catttcgcac aacctggcgc agcgtaaagc cgcagcggtt gaggcggaaa ctattgtcgc
     7561 tcaggaaacc agcgaattta tggcgtggct gcgagcacaa agcgccagcg aaaccattcg
     7621 cgagtatcgc agccaggcag agcacgttcg cgatgagtta accgccaaag cgttagcggc
     7681 ccttgagcag ggcggcgacg cgcaagccat tatgcaggat ctggcatgga aactgactaa
     7741 ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc gcccgtgacg gggataacga
     7801 acgcctgaat attctgcgcg acagcctcgg gctggagtag cagtacatca ttttcttttt
     7861 ttacagggtg catttacgcc tatgaagcct tctatcgttg ccaaactgga agccctgcat
     7921 gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc aaactatcgc cgaccaggaa
     7981 cgttttcgcg cattatcacg cgaatatgcg cagttaagtg atgtttcgcg ctgttttacc
     8041 gactggcaac aggttcagga agatatcgaa accgcacaga tgatgctcga tgatcctgaa
     8101 atgcgtgaga tggcgcagga tgaactgcgc aaagctaaag aaaaaagcga gcaactggaa
     8161 cagcaattac aggttctgtt actgccaaaa gatcctgatg acgaacgtaa cgccttcctc
     8221 gaagtccgag ccggaaccgg cggcgacgaa gcggcgctgt tcgcaggcga tctgttccgt
     8281 atgtacagcc gttacgccga agcccgccgc tggcgggtag aaatcatgag cgccagcgag
     8341 ggtgaacatg gtggttataa agagatcatc gccaaaatta gcggtgatgg tgtgtatggt
     8401 cgtctgaaat ttgaatctgg cggtcatcgc gtgcagcgtg ttcctgctac ggaatcgcag
     8461 ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc cagaactgcc tgacgcagaa
     8521 ctgccggaca tcaacccagc agatttacgc attgatactt tccgctcgtc aggggcgggt
     8581 ggtcagcacg ttaacaccac cgattcggca attcgtatta ctcacttgcc gaccgggatt
     8641 gttgttgaat gtcaggatga acgttcacaa cataaaaaca aagcgaaggc gctttcagtg
     8701 ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc aacaggccga agcgtctacc
     8761 cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta accgtactta caatttcccg
     8821 caggggcgcg ttaccgatca ccgcatcaac ctgacgctct accgcctgga tgaagtgatg
     8881 gaaggtaagc tggatatgct gattgaaccg attatccagg aacatcaggc cgaccaactg
     8941 gcggcgttgt ccgagcagga ataatggaat atcaacactg gttacgtgaa gcaataagcc
     9001 aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat cctgctggag catgttaccg
     9061 gcagagggcg tacttttatt ctcgcctttg gtgaaacgca gctgactgac gaacaatgtc
     9121 agcaacttga tgcgctactg acacgtcgtc gcgatggtga acccattgct catttaaccg
     9181 gggtgcgaga attctggtcg ttgccgttat ttgtttcgcc agcgacctta attccgcgcc
     9241 cggatacgga gtgtctggtg gagcaggcac tggcgcggtt gcctgaacaa ccttgccgta
     9301 ttcttgatct cgggacgggt accggggcga ttgcgcttgc gctggctagc gagcgcccgg
     9361 actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt ctccctggca caacgtaatg
     9421 cccagcatct ggcgatcaaa aatatccaca ttctgcaaag cgactggttt agcgcgctag
     9481 ccgggcagca gtttgcgatg attgtcagca atccgccgta tattgacgag caggacccac
     9541 atcttcaaca aggcgatgtc cgctttgagc cgctcactgc gctggttgcg gcagacagtg
     9601 gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa cgcgctggta tccggcggct
     9661 ttctgcttct ggaacatggc tggcagcagg gcgaagcggt gcgacaagca tttatcctcg
     9721 cggggtatca tgacgtcgaa acctgccgtg actatggtga taacgagcgc gtaacgctcg
     9781 gccgctatta tcaatgacaa gtttttctac actgcttagt gttcatctta ttagtatcgc
     9841 gctttctgtt gggctattaa ccttacgttt ctggctacgt tatcagaagc atccacaggc
     9901 atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat acggtgttat tgttaagcgg
     9961 cattgcgttg atggctaaag cgcacatcct gccattttcc gggcaggcac agtggctgac
    10021 tgaaaagctg tttggagtta tcatttatat cgttttgggt tttattgcac tcgattatcg
    10081 tcgtatgcac agtcagcagg cgcgcattat tgccttcccg ctggcgttgg tggtgctgta
    10141 catcatcatt aaactcgcca ccacaaaagt accgttactg gggtaagtca tgagatcgtt
    10201 agctgatttc gaatttaata aagcgccatt gtgcgaaggc atgatcctgg cttgcgaagc
    10261 aatccgccgc gattttccct cgcaagatgt ttacgacgaa ctggagcgtc tcgttagtct
    10321 ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag cagctggaaa aactgatcgc
    10381 gctgttttac ggcgactggg gatttaaagc ctcacgcggc gtttatcgtc tttccgatgc
    10441 attatggctg gaccaggtgt taaagaatcg acagggcagt gcggtatcat taggtgcggt
    10501 tttattatgg gtcgcgaatc gtctcgattt gccgctgctg ccggtgattt tccctacgca
    10561 gctgatattg cgcattgaat gtccggatgg cgaaatttgg ctgattaatc cttttaacgg
    10621 tgaatcgtta agcgaacata tgctggacgt atggttaaag ggaaatatca gcccgtcggc
    10681 ggaactgttt tatgaagacc ttgatgaagc tgataacatt gaggtaatcc gcaaattgct
    10741 ggatacactc aaagcctcgt tgatggaaga aaatcagatg gagctggcgt tacgcaccag
    10801 cgaagcttta ttacaattca accctgaaga tccctatgaa attcgcgatc gcgggttgat
    10861 ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat ttaagttatt tcgttgaaca
    10921 gtgtccggaa gacccgatca gcgaaatgat ccgtgcgcaa ataaataaca tcgcgcataa
    10981 acatattgtg ctgcattaat taatcgacat tttactcaag attaaggcga tcctatgaaa
    11041 caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg acctgccgtt cgtactgttt
    11101 ggcggtatga acgtgctgga atctcgcgat ctggcgatgc gcatttgcga gcactacgta
    11161 actgtgaccc agaaactggg tatcccttac gtgttcaaag cctcttttga caaagccaac
    11221 cgctcctcca tccactctta tcgtggaccg ggcctggaag aagggatgaa aatcttccaa
    11281 gagttgaagc agacttttgg cgtgaaaatt atcaccgacg ttcacgaacc aagtcaggca
    11341 cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg cgtttcttgc tcgccagact
    11401 gacctggttg aagccatggc gaaaaccggt gcggtaatta acgtcaagaa accacagttt
    11461 gtcagcccgg gacagatggg taatatcgtt gataaattca aagaaggcgg caacgaaaaa
    11521 gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca acctggttgt cgatatgctg
    11581 ggcttcagca ttatgaagaa agtgtctggt aactcgccgg tgattttcga cgtgacccac
    11641 gcactgcaat gccgcgatcc gtttggcgca gcttccggtg gtcgtcgtgc tcaggtggct
    11701 gagctggcac gagccggtat ggcggtaggt ctggcggggc tgtttattga agcgcatccg
    11761 gatccggaac atgcgaaatg tgatggtcca tccgcgctgc cgctggctaa actggaaccg
    11821 ttcctcaagc agatgaaagc gattgatgat ctggtgaaag gtttcgaaga actggatacc
    11881 agcaagtaat cttttttgct tgaaaaataa agtcttagcg ttctgcgtta agactttttt
    11941 catgggtgcc agatacaaaa aaggccgcag gctgttaccc ctgcggccgg tttcgggcgc
    12001 atattgccat cacggcagcc tgacgcccgt tttcacctta cttccggtta cgccaccagc
    12061 cgacaatcgc tgcggtaata attcccgcca ggatcggtgc tgccaggtcg tgccagaaaa
    12121 tcatggcaaa ctgcgcgagc gtcatatagc cgccttgttg taatgacaac attttgcggc
    12181 tattcttgaa ttgttctggt tcaagattag cccccg
//
LOCUS       DAJTNN010000125        12089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTNN010000125 DAJTNN010000000
VERSION     DAJTNN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.125"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(245..559)
                     /locus_tag="OO150_002986"
                     /pseudo
     CDS             complement(245..559)
                     /locus_tag="OO150_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(674..1072)
                     /locus_tag="OO150_002987"
     CDS             complement(674..1072)
                     /locus_tag="OO150_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HCR5517058.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESAPGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1312..3726)
                     /gene="gyrB"
                     /locus_tag="OO150_002988"
     CDS             complement(1312..3726)
                     /gene="gyrB"
                     /locus_tag="OO150_002988"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5517059.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3755..4828)
                     /gene="recF"
                     /locus_tag="OO150_002989"
     CDS             complement(3755..4828)
                     /gene="recF"
                     /locus_tag="OO150_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5517060.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLVEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4828..5928)
                     /gene="dnaN"
                     /locus_tag="OO150_002990"
     CDS             complement(4828..5928)
                     /gene="dnaN"
                     /locus_tag="OO150_002990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5517061.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5933..7336)
                     /gene="dnaA"
                     /locus_tag="OO150_002991"
     CDS             complement(5933..7336)
                     /gene="dnaA"
                     /locus_tag="OO150_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5517062.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7633..7713
                     /locus_tag="OO150_002992"
     CDS             7633..7713
                     /locus_tag="OO150_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517063.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7943..8083
                     /gene="rpmH"
                     /locus_tag="OO150_002993"
     CDS             7943..8083
                     /gene="rpmH"
                     /locus_tag="OO150_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5517064.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            8100..8459
                     /gene="rnpA"
                     /locus_tag="OO150_002994"
     CDS             8100..8459
                     /gene="rnpA"
                     /locus_tag="OO150_002994"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5517065.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8423..8680
                     /gene="yidD"
                     /locus_tag="OO150_002995"
     CDS             8423..8680
                     /gene="yidD"
                     /locus_tag="OO150_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5517066.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8683..10329
                     /gene="yidC"
                     /locus_tag="OO150_002996"
     CDS             8683..10329
                     /gene="yidC"
                     /locus_tag="OO150_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5517067.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10435..11799
                     /gene="mnmE"
                     /locus_tag="OO150_002997"
     CDS             10435..11799
                     /gene="mnmE"
                     /locus_tag="OO150_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5517068.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            12042..>12089
                     /gene="tnaC"
                     /locus_tag="OO150_002998"
     CDS             12042..>12089
                     /gene="tnaC"
                     /locus_tag="OO150_002998"
                     /inference="COORDINATES: protein motif:HMM:NF019665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5517069.1"
                     /translation="MNILHICVTSKWFNID"
BASE COUNT         2776 a         3296 c         3140 g         2877 t
ORIGIN      
        1 ccgggccatt gcggggctga atgtggaggg gctacgcgcg ggatatcagt ctgctgaggt
       61 atatgccggc ctgacacagg ctaagtgtgc ccgggaatcg gtgcaagtat aaaaaacagc
      121 ctgttgcggt ataagaatcg accatgcaga gagagaaaac accggagtgg agggaaaagc
      181 agaaaagctc acgtggcata aggcgcggac aggggtatcg cttagttttt caatttccta
      241 tacgtgaacc gacttcgcga gagagttttt ccaggaaacg atagtcgtca taactgagag
      301 cagtttgcgc cacggtgcta ccatcagcgg ccttctgtac cagcgcgccg ttataagtaa
      361 tgcagtagtc gcccggctgt tccatatgca gctctttcag gtagttgtga acgcctgcat
      421 aagggcgacc cgtcgttagc acgacattca cgccacgggc gcgagctgcg gcaatcgcat
      481 ttttaacggc gggtgaaatg gtgtgatcgg gcagcagaag ggtgccatcc atatcgatag
      541 caatgagttt gatagccatg agttccccag gtagattagt tcctgaccca tgctaacgcg
      601 attccgctca aaaatcagta caacacccga gggaaaaggg ggatgcaacg cgggttgcat
      661 gctccccatt tgcttagcgg aagagtttcc ctttcagcag ttccatacct gcggaaagca
      721 gatcgttatt ggcttgtgaa gacacttcac cttgcggtga gagcgcatca ataatcttcg
      781 gcaattgttc tgccagtaaa ctggaagccg tactggtatc cacgccaagt ttttgcccga
      841 gatcggacac cgcatttgtg ccgggcgccg attccagttg ctcgccgcta actgattgat
      901 tgctctgctg attactcaac caggttgaga gaatggcccc taagccgcca ctttgcagtt
      961 tttccagcag cacctgaatg ccgccctgct cctcaaccca acttaaaata gcctgatatt
     1021 tccccgcatc gcctttcaga aaggcaccga caacttcatc aaaaagcccc atgataatca
     1081 cctgtaaagc gttacgtgtt gacccaaaaa gtatagatct gcgggatgat aattgcggat
     1141 tgcagaaata aaaagggcgg agatgatctc cgcccttttt ttatagcttc ttgccggatg
     1201 cggcgttaac gtcttacctg gcctacaaaa tcattaaaat tcaatacatt gcaagatttt
     1261 cgtaggcctg ataagcgtag cgcatcaggc acgctcgcat ggttaacgcc attaaatatc
     1321 aatattcgcc gctttcaggg cgttctcttc aataaacgca cgacgcggtt ctacggcgtc
     1381 gcccatcagc gtggtgaaca acaggtcagc ggcaatcgca tccttaacgg taacgcgcag
     1441 catacgacga ctttccgggt ccatggtggt ttcccacagc tgttccgggt tcatctcgcc
     1501 cagaccttta taacgctgga tggagagacc gcgacgagac tctttcacca gccagtccag
     1561 cgcctgctcg aagctggcta ccggctgacg acgctcgcca cgttcgataa atgcatcttc
     1621 ttccagcaag ccacgcagtt tctcacccag cgtgcagata cgacgatatt cgccaccagt
     1681 gataaattcg tgatccagcg gatagtcagt atccacaccg tgggtacgca cgcgaacaat
     1741 cggctcgaac aggttttgtt cggcattggt gtggacatca aacttccact ggctgccgtg
     1801 ctgttctttg tcgttcagtt cgctgaccag cgcgttcacc cagcgggtaa cggtctgctc
     1861 atcagagagg tcggcttccg tcagcgtcgg ctgatagata agctctttca gcattgcttt
     1921 cggataacga cgctccatgc ggttgatcat tttctgcgtc gcgttgtact cagacaccag
     1981 tttctctaac gcttcgccag ccaatgccgg tgcgctggcg ttggtgtgca gcgttgcgcc
     2041 gtccagcgcg atagagatct ggtactgatc catcgcttcg tcgtctttaa tgtactgttc
     2101 ctgcttgcct ttcttcactt tgtacagcgg cggctgagcg atgtagacgt gaccgcgttc
     2161 aacgatttcc ggcatctgac gatagaagaa ggtcaacagc agcgtacgaa tgtgcgagcc
     2221 gtcgacgtcc gcatcggtca tgatgatgat gctgtgataa cgcagtttgt ccgggttgta
     2281 ctcgtcacga ccgataccac agccaagcgc ggtgataagc gtcgccactt cctgagaaga
     2341 gagcatctta tcgaagcgcg ctttctcaac gttgaggatt ttacccttca gcggcagaat
     2401 cgcctggttc ttgcggttac gcccctgctt cgcagagccg cccgcggagt ccccttccac
     2461 caggtacagt tcggaaagcg ccggatcgcg ttcctggcag tctgccagtt tgcccggcag
     2521 gcccgctaag tcgagcgcac ctttacggcg ggtcatttca cgcgcgcgac gcgccgcttc
     2581 acgggcacgg gcagcatcga taattttgcc aaccacgatt ttcgcgtcgg ttgggttttc
     2641 cagcaggtat tctgccagca gttcgttcat ctgctgttca accgccgatt tcacctcaga
     2701 agaaaccagt ttgtctttgg tctgggagga gaatttcggg tccggcactt ttacggaaac
     2761 gaccgcaatc aggccttcac gcgcatcgtc accggtggcg ctgactttgg cttttttgct
     2821 gtagccttct ttgtccatgt aggcgttcag ggtacgggtc atcgccgcac ggaagcctgc
     2881 caggtgagta ccgccgtcac gctgcggaat gttgttggta aagcagtaga tgttttcctg
     2941 gaagccatcg ttccactgca acgccacttc gacgccaata ccgtcttttt cggtggagaa
     3001 gtagaagata ttcggatgga tcggcgtttt gttcttgttc agatattcaa cgaacgcctt
     3061 gatgccgcct tcatagtgga agtggtcttc tttgccgtca cgcttgtcgc gcagacgaat
     3121 ggaaacgccg gagttgagga acgacaactc acgcagacgt ttcgccagaa tttcatattc
     3181 gaactcggtc acattggtga aggtttcgag gctgggccag aaacgcacca tggtgccggt
     3241 tttttcagtc tcgccggtaa ccgccagcgg agcctgcggt acaccgtgtt cgtagatctg
     3301 acggtgaatt ttaccctcgc gctggataac cagctccagt ttttgcgaca gggcgtttac
     3361 taccgaaaca ccaacgccgt gcagaccgcc ggacacttta taggagttat cgtcaaattt
     3421 accgcctgcg tgcagaacgg tcatgatcac ttccgccgcc gatacgccct cttccgggtg
     3481 aataccggtc ggaatgccgc gcccgtcatc ctgtacagag acagagttat cggcgtgaat
     3541 ggtgacgata atttctttac agtgacccgc gagcgcttcg tcgatagcgt tatctaccac
     3601 ctcgaatacc atgtggtgca gaccggtgcc gtcatccgtg tcgccgatat acatacccgg
     3661 gcgcttacgc accgcatcca gccctttcag gactttgata ctggaggagt cataagaatt
     3721 cgacatcaac gtttctcgct catttatact tgggttaatc cgttatttta cccttttcca
     3781 cggtaaacat cttcgaattt tcgtccgaca tgtctataac gtgttcagca ctgatcgcgc
     3841 tgacaaagac ctgtgattgc gtcgctttta agcggctggc aagcagcccg cgacgctcat
     3901 catcaagctc agaggcaaaa tcatctatca ggtagagaca ccgccgcccg ctttcacggg
     3961 tgaggaactc tccttgcgcc agacgtaagg cgcacatcaa cagcttaagc tgcccacgcg
     4021 ataaggtatc ttccaccggc gcaccgtcgg cgcgaatgcg taagtccgct ttatgcgggc
     4081 catgcgcggt gtaggttagc tggcgatcgc gttcaaaatt acgttccagc acctcagcat
     4141 attctgtctc tttctcccag ccgcgctgga aagagaaagt cagagaaaac tcagggagaa
     4201 attgcttaca agtatcggcc atatcagccg cgataccggc gctatactcc gcgcgccagg
     4261 tgctgatttg ctccaccagc gggatcagct ctttatccca cgggcgtagc tgttcgtaac
     4321 gtgtcacctg gcgcagcgcc gcattgcgct gcttgagcaa tcgcttgaga ttgctccagg
     4381 cggtgaaaaa tccgggttcg ttgtgaaagc atgcccagtc gagaaatgct cttctgtatt
     4441 tggggccgcc gttgagtaaa gtaaaccctt ctggcgttat cagctgcatc ggcatcaggt
     4501 gcgccagctc cgcgacttta tgcccgtcag taccgtcgat gcggactttg ctgtcgcctt
     4561 gtttgtcttt ggttaagcca atcgccgtct cgcgctcttc gccctgtaat cgcccatgga
     4621 gaacaaatgc ctcctgctca tggcgaatga cgcgaccaat ctgcaaactg cgaaacgccc
     4681 gaccatggcc gagcgtatag atggcttcca gcacactggt tttgccactg ccgttggcac
     4741 ctaccagaaa gttaaagccg ggagataagg cgagatccgc ggtttcgata ttgcggaaat
     4801 cgcggatcaa caagcgggtg agggacatta cagtctcatt ggcatgacaa cataagccgc
     4861 gctctggctg gccgcatctt caatctgcac gctggaaacc gaatcggtca gcatcatgcg
     4921 gacgttttcg catttcagcg cgttcagaac atccagcaca tagctgacgt tgaagccgat
     4981 ttccatctcc gcaccgctat aggtaacgtc gaggatctct tccgcttctt cctgttccgg
     5041 gttgttggca gtgattttca gctggttttc gctgacataa agacgtacgc cgcggaattt
     5101 ctcgttagag agaattgccg cacgggcaaa cgcctgcttg agcagatcgc agccagcttc
     5161 cagatgtttg tccgggttct tcggtagaac gcggcggtaa tccgggaagc gaccatccac
     5221 cagtttggag gtgaagataa agtcgccaac gtgggcacga atgttattgc tgccaatctg
     5281 cacgcgcagc ggattgtcgc cgccgtcgag catacgcatc agttcaatca cgcctttacg
     5341 cggcacgatc accgaatggc ttggcaaaga ttgaccaatt ggcattgaac agactgccag
     5401 acggtgacca tcggtcgcta cggtgcgcag ttcttcgcct tcggtttcaa acagcatacc
     5461 gtttaagtaa tagcgaacat cctgatgcgc catcgaaaac tgggtcgctt caatcagacg
     5521 cttcatcgtc gcctgcggca gggtaaattc gacttcgctc tgccagtcat cgaggttcgg
     5581 gaaatccgcc gctggcaggg tagacagcga aaaacggcta cgcccggagc gcaccagcat
     5641 ccgttcacct tccagctgca cggcaatttc cgcgccttca ggcagaccac ggcagatatc
     5701 aaagaatttg cgcgccggaa cggtcgttgc tcctggctcg tgtggctgaa ccagcgcaac
     5761 acgtgccacc atttccatct cgagatcagt accggtcagc gacaacgtac cgtcagcaac
     5821 ctgtaacagc agattaccga gaatcggtag cgtaggacga ccacctaacg gaccgctcac
     5881 ctgttgtagc ggttttaata aatgctcacg ttctacggta aatttcatag gtttacgatg
     5941 acaatgttct gattaaattt gaaaaatctt ctttgatatc gtggctctct tcacgcaact
     6001 gctcgatctt acggcaggca tgaagcaccg tcgtgtggtc acgaccacca aacgcatcgc
     6061 caatctccgg cagactgtgg ttagtcagct ctttcgccag cgccatcgcc atctggcgcg
     6121 gacgcgccac cgagcgggat cgacgcttgg aaaggagatc cgcgactttg atcttgtagt
     6181 actccgccac cgtcttctga atattgtcga tggtgaccag tttttcctgc aatgccagca
     6241 agtcgcgcag cgcctcacgc acgaagtcga tggtgatcgc ccgtccggta aagttggcat
     6301 tggcaatgac gcggttcagc gccccttcca gctcacgtac gttagatcgt agacgcttgg
     6361 cgataaagaa cgccacttcg cccggcaaac gaatgtcgtt ttcgtcggcc tttttcatca
     6421 ggatcgccac acgggtttcc agctctggcg gttcgatcgc cacagtcagt ccccaaccga
     6481 agcgggattt caaacgatcc tcaacgccgt tgatctcttt cggatagcga tccgaggtga
     6541 gaatgatctg ttgattacct tccagcaggg cgttgaaggt atggaaaaac tcttcctgag
     6601 atcgttcttt attagcaaaa aactgaatat cgtcgatcag cagtgcatct acggaacggt
     6661 agtagcgttt aaactcttcg atcgcgttgt tttgcagggc tttaaccatg tcctgaacaa
     6721 agcgctcgga gtgcatataa accactttgg cattcggctt gcgcgccata atgccgttac
     6781 ccaccgcatg cagtaggtga gttttaccca gacccgtgcc gccataaagg aacaacgggt
     6841 tataggcacc gccagggtta tccgccacct ggcgagccgc cgcgcgcgcc agttggttag
     6901 atttaccttc aacgaagtta tcaaacgtgt gtttgacgtt tacgttagaa cgataggtcg
     6961 gttctgccgg ggccgggacg ttatcccaac ctgagcgcgt agaaggcgca gcacgttgcg
     7021 gctgcgtttg cgccacctgt gcaggggccg cgacgttgct cgtcactgcc gcttgtggcg
     7081 tttgcgtcac cggtttggtg ccgacttcaa aacgcagctg tggggcatcc gctccgcaga
     7141 aactggttag cagtccattg atattattaa ggtacttgtc ccgtacccaa tcgaggacaa
     7201 aacggtttgg cgcgtacagg gccagcgtgt tatcgctcag ttccgcctgc aatgggcgta
     7261 tccacatact gaattctgtg gctggtaact catcctgcaa tcgggcaaga cactgctgcc
     7321 aaagcgaaag tgacacggcg gactccactc gaacaaaagt cgataatgac taaggctgaa
     7381 acattcatga ttgttgacgt acgtcgaaaa gaccctgctt gagggtgacg cacgaaccgc
     7441 tgtctgcggt tatatgcccg atcaagatcc tgcaaaacga tcgggaccgc ggatcatagc
     7501 ctaaactgcg caagagatct tctgtttctc acagattttc ccgatttatc cacaggactt
     7561 tccagaactc gctaagtgta aacgatcctg ccgcgaggcg ggcacgattt acgccacata
     7621 ttgaaaaatt taatgaccat agacaaaaat tggcttaatc gatctaataa agatccagga
     7681 cgatccttgc gctttaccca tcagcccgta taatcctcca cccggcgcgc catgctggtt
     7741 tccactggtg tgaggtcgta cattttccct gcgaaaaggt gcggaaaagc gcggtaaata
     7801 aggaaagaga attgactccg gagtgtacaa ttattacaat ccggcctctt taatcaccca
     7861 tggcttcggt gtccatcgtt tcatttttcg gcggatatcc aataaagcca ttgaatttat
     7921 tcaagtttag gtagaaatcg ccatgaaacg cacttttcaa ccgtctgtac tgaagcgcaa
     7981 ccgttctcac ggcttccgtg ctcgtatggc tactaaaaat ggtcgtcagg ttctggcacg
     8041 tcgtcgtgct aaaggccgcg ctcgtctgac cgtttctaag taatcaagct aacccctgag
     8101 tggttaagct cgcatttccc agggagttac gcttgttaac tcccagtcaa ttcacattcg
     8161 tcttccagca gccacaacgg gctggcacgc cgcaaattac cattctcggc cgcctgaatt
     8221 cgctggggca tccccgtatc ggtcttacag tcgccaagaa aaacgttcga cgcgcccatg
     8281 aacgcaatcg gattaaacgt ctgacgcgtg aaagcttccg tctgcgccaa catgaactcc
     8341 cggctatgga tttcgtggtg gtggcgaaaa aaggggttgc cgacctcgat aaccgtgctc
     8401 tctcggaagc gttggaaaaa ttatggcgcc gccactgtcg cctggctcgc gggtcctgat
     8461 agccctcatt cgggtctatc aacgcctgat tagtccgcta ctcgggccgc attgtcgttt
     8521 cactccaacc tgttcaagct acggaattga ggcattgcgc aggtttggag tgataaaagg
     8581 cagttggttg acggtgaaac gcgtattaaa atgccaccct ttacaccctg gtggtgacga
     8641 tcccgtcccg cccggaccat ttgataccag agaacactaa cgatggattc gcaacgcaat
     8701 cttttagtca tcgctttgct gttcgtgtct ttcatgatct ggcaagcctg ggagcaggat
     8761 aaaaacccgc aacctcaggc ccaacagacc acgcagacaa cgaccaccgc agcgggtagc
     8821 gccgccgacc agggcgtacc ggccagtggc caggggaaac tgatctcggt taagaccgac
     8881 gtgcttgatc tgaccatcaa cacccgtggt ggtgatgttg agcaagctct gctgcctgct
     8941 tacccgaaag agctgaactc tacccagccg ttccagctgc tggaaacttc accgcagttt
     9001 atttatcagg cacagagcgg tctgaccggt cgtgatggcc cggataaccc ggctaacggc
     9061 ccgcgtccgc tgtataacgt tgaaaaagac gcttatgtgc tggctgaagg tcaaaacgaa
     9121 ctgcaggtgc cgatgacgta taccgacgcg gcaggcaaca cgtttaccaa aacgtttgtc
     9181 ctgaaacgtg gtgattacgc tgtcaacgtc aactacaacg tgcagaacgc tggcgagaaa
     9241 ccgctggaaa tctccacctt tggtcagttg aagcaatcca tcactctgcc accgcatctc
     9301 gataccggaa gcagcaactt cgcactgcac accttccgcg gcgcggcgta ctccacgcct
     9361 gacgagaagt acgagaaata caagttcgat accattgccg ataacgaaaa cctgaacatc
     9421 tcttcgaaag gtggttgggt ggcaatgctg caacagtatt tcgcgacggc gtggatcccg
     9481 cataacgacg gtaccaacaa cttctatacc gctaatctgg gtaacggcat cgccgctatc
     9541 ggctataaat ctcagccggt actggttcag cctggtcaga ctggcgcgat gaacagcacc
     9601 ctgtgggttg gcccggaaat ccaggacaaa atggcagctg ttgctccgca cctggatctg
     9661 accgttgatt acggttggtt gtggttcatc tctcagccgc tgttcaaact gctgaaatgg
     9721 atccatagct ttgtgggtaa ctggggcttc tccattatca tcatcacctt tatcgttcgt
     9781 ggcatcatgt acccgctgac caaaacgcag tacacctcca tggcgaagat gcgtatgctg
     9841 cagccgaaga ttcaggcaat gcgtgagcgt ctgggcgatg acaaacagcg tatcagccag
     9901 gaaatgatgg cgctgtacaa agctgagaag gttaacccgc tgggcggctg cttcccgctg
     9961 ctgatccaga tgccaatctt cctggcgttg tactacatgc tgatgggttc cgttgaactg
    10021 cgtcaggcac cgtttgcact gtggatccac gacctgtcgg cacaggaccc gtactacatc
    10081 ctgccgatcc tgatgggcgt aacgatgttc ttcattcaga agatgtcgcc gaccacagtg
    10141 accgacccga tgcagcagaa gatcatgacc tttatgccgg tcatcttcac cgtgttcttc
    10201 ctgtggttcc cgtcaggtct ggtgctgtac tatatcgtca gcaacctggt aaccattatt
    10261 cagcagcagc tgatttaccg tggtctggaa aaacgtggcc tgcatagccg cgagaagaaa
    10321 aaatcctgat tcggtgagtt ttcgctaaaa taagggcggt cagttgaccg ccttttttct
    10381 tttcgtaggg cggataagca ccgcgcatcc gccacacaaa gcaacaggaa catcatgagc
    10441 gataatgaca ctatcgtagc ccaggccacg cctccgggac gtggcggcgt tggcatcctg
    10501 cgcatctccg gcttcaaagc ccgtgaagtt gccgaaaccg tgctgggtaa actgcctaag
    10561 ccgcgctacg ccgattatct tccgtttaaa gacgccgacg gcagcgtgct cgatcagggg
    10621 attgcgctat ggttccctgg cccgaactcg ttcaccggcg aagatgtgct ggaactgcaa
    10681 ggtcatggcg gtccggtgat cctcgacctg ctgttaaaac gtattctgac cattcccggc
    10741 ctgcggattg ctcgccctgg tgagttttcc gaacgcgcgt ttcttaacga taaacttgac
    10801 ttagcccagg ccgaggcgat tgccgatctt atcgacgcca gttcggaaca ggcggcccgt
    10861 tcggcactta actcgctgca aggcgcattc tccgcacggg ttaatcatct ggtagaagcc
    10921 ctcacccact tgcgcattta cgtcgaagcg gcaattgatt tccccgatga agagatcgat
    10981 ttcctctccg acggaaaaat tgaagcccag ctcaatgacg ttattgccga tcttgatgca
    11041 gtgcgtgctg aagcacgtca gggtagtttg ttgcgcgaag ggatgaaagt ggtgattgcc
    11101 ggacgtccta acgccggtaa atcgagcctg ttaaacgcgc tggcggggcg tgaagcggca
    11161 atcgtaaccg atatcgccgg aactacgcgt gacgtgctgc gtgagcatat ccacattgac
    11221 ggaatgccgc tgcatatcat cgataccgcc gggctacgtg aagccagtga cgaagtagaa
    11281 cgtattggta tcgagcgcgc gtggcaggaa attgaacagg ccgaccgcgt gctgtttatg
    11341 gtcgatggca ccacaacaga cgccgtggat ccggcagaga tctggccgga atttattgcc
    11401 cgtctgccag cgaaactgcc gatcaccgtg gtgcgcaata aagccgatat caccggcgaa
    11461 acgctgggaa tgagtgaagt gaacggtcac gcgttaattc gtctctcggc aaggactggt
    11521 gaaggcgtgg acgtgctgcg taaccatctc aaacagagca tgggctttga caccaacatg
    11581 gaaggcggct tcctggcgcg tcgtcgccac ctacaggcgc tggaacaggc agcggaacat
    11641 ctacaacagg gcaaagcgca actgttggga gcctgggcag gtgaactgct ggcggaagag
    11701 ttgcgtctgg cacagcagaa cttaagcgaa atcaccgggg aatttacttc agacgacctg
    11761 ctggggcgga ttttctccag cttctgtatt ggtaagtaac cgcgcttacg aagccgtatt
    11821 ctgactgtca gatgcggctt cgcttcattg ttagcactcc tgttattcct caaccctttt
    11881 tttaaacatt aaaattctta cgtaatttat aatcttaaaa aaagcattta atattgctcc
    11941 ccgaacgatt gtgattcgat tcacatttaa acaattttag aatagacaaa attcttgagt
    12001 attatactgt agcctcgtgt ctttcgagga ataagtgcat tatgaatatc ttacatatat
    12061 gtgtgacctc aaaatggttc aatatcgac
//
LOCUS       DAJTNN010000126        12050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTNN010000126 DAJTNN010000000
VERSION     DAJTNN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.126"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            14..100
                     /locus_tag="OO150_002999"
     tRNA            14..100
                     /locus_tag="OO150_002999"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48..50,aa:Leu,seq:cag)
     gene            136..222
                     /locus_tag="OO150_003000"
     tRNA            136..222
                     /locus_tag="OO150_003000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:170..172,aa:Leu,seq:cag)
     gene            complement(261..497)
                     /locus_tag="OO150_003001"
     CDS             complement(261..497)
                     /locus_tag="OO150_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5517070.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            638..1426
                     /gene="fhuF"
                     /locus_tag="OO150_003002"
     CDS             638..1426
                     /gene="fhuF"
                     /locus_tag="OO150_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5517071.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1464..2140)
                     /gene="bglJ"
                     /locus_tag="OO150_003003"
                     /pseudo
     CDS             complement(1464..2140)
                     /gene="bglJ"
                     /locus_tag="OO150_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(2098..2823)
                     /locus_tag="OO150_003004"
     CDS             complement(2098..2823)
                     /locus_tag="OO150_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5517072.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3442..4212
                     /locus_tag="OO150_003005"
     CDS             3442..4212
                     /locus_tag="OO150_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5517073.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAIITFFASTAAMYIRQLLAQ
                     HHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            4203..4676
                     /locus_tag="OO150_003006"
     CDS             4203..4676
                     /locus_tag="OO150_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5517074.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTRGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4784..5323
                     /gene="dnaT"
                     /locus_tag="OO150_003007"
     CDS             4784..5323
                     /gene="dnaT"
                     /locus_tag="OO150_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5517075.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5326..6063
                     /gene="dnaC"
                     /locus_tag="OO150_003008"
     CDS             5326..6063
                     /gene="dnaC"
                     /locus_tag="OO150_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5517076.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            6112..6606
                     /locus_tag="OO150_003009"
     CDS             6112..6606
                     /locus_tag="OO150_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5517077.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6860..9151
                     /gene="opgB"
                     /locus_tag="OO150_003010"
     CDS             6860..9151
                     /gene="opgB"
                     /locus_tag="OO150_003010"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5517078.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9290..10312)
                     /gene="lgoD"
                     /locus_tag="OO150_003011"
     CDS             complement(9290..10312)
                     /gene="lgoD"
                     /locus_tag="OO150_003011"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR5517079.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGLQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10451..11365
                     /locus_tag="OO150_003012"
     CDS             10451..11365
                     /locus_tag="OO150_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5517080.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            11580..>12050
                     /locus_tag="OO150_003013"
     CDS             11580..>12050
                     /locus_tag="OO150_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5517081.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVIND"
BASE COUNT         3122 a         3070 c         2834 g         3024 t
ORIGIN      
        1 ttgctcgcac tgggcgaagg tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt
       61 tcttacggac gtgggggttc aagtcccccc cctcgcacca aacgaggcga tatcaaaaaa
      121 aagtaagatg attgtgcgaa ggtggcggaa ttggtagacg cgctagcttc aggtgttagt
      181 gtccttacgg acgtgggggt tcaagtcccc cccctcgcac caattatctt tacttccttt
      241 cttgtttctt ccttgatttt tcatccctga ttcaaattca ttatcatcag catgatggca
      301 gcaataagcg ccaggcacga tggtagcaaa acgtaatgcc gtaactgttt gtgatacagc
      361 atcaccggcg tcaatactaa tcccatcaga atgacaatcc gccactgatc cggcgataaa
      421 tccgcataca tcagccctgc aatcagcaat cccggcagca acactcccca gccactgccc
      481 agcgtacgtt gcaacatggt ttcatctctt tcattgataa tgataactaa tatcatatga
      541 taatttttat catttgcaag ccagagaaat cccttgctat cgggtaaacc tatcgctagg
      601 attagcaatc attatcattt agattactat cccgattatg gcctatcgtt ccgcaccgct
      661 ctatgaagat atcatctggc gaacgcatct ccagccgcag gatgctgggc ttgcacaagc
      721 cgtacgtgcg acgatcgcca aacatcgtga acatttgctc gagtttatcc gcctggatga
      781 acccgctccg cttaacgcca tgacgctggc gcaatggtca tcaccgaatg cgctaagttc
      841 tctactcgcg gtctattccg atcatatcta tcgcaaccaa ccaacgatga tccgcgagaa
      901 caaaccgctg atctcactat gggcacaatg gtatatcggc ctgatggtgc caccattaat
      961 gctggcgcta ctgacgcagg aaaaggcatt agatgtgtcg ccggaacatt tccatgctga
     1021 gtttcacgaa accggacgcg tcgcctgttt ctgggtcgat gtgtgtgaag ataaaaacgc
     1081 aacaccacat tcgccgcagc agcgaatgga aacgttaatc agccaggcgc tggttccggt
     1141 tgtgcaggca ctagaagcga ccggagagat caacggcaaa cttatctgga gtaataccgg
     1201 ttatttgatc aactggtatc tcactgagat gaagcaactg ctcggcgagg cgacggttga
     1261 atcgctgcgc catgccctct tttttgagaa aacgctcacg aacggtgaag ataatccact
     1321 ctggcgtacc gtcgtgctgc gcgatggcct gctggtgcgc cgaacctgct gccagcgtta
     1381 tcgcttaccg gatgtgcagc aatgtggcga ttgtacgctg aaatgaaagc actgccgggg
     1441 aagtaaaccc ggcatcatgc ggattaatag ggatgcaaca cattacttgc ttcgcaatgc
     1501 cgcatacata acagaatatc tgcggcctcc aacacccctg catcagaact gacgcccagt
     1561 ttcgacatca cattaaattt atgtgcacgg atcgttttga tattgcgttt aagctgctcg
     1621 gcaatttgtg tcattgagta gccacgcgac ataaagcgca atatttctct ctccgtcggg
     1681 cttagcgtcc ggctttggtt aatgtaccac tgattgttca gtcggtcggt cgcctgacgt
     1741 acaccattta atgacaaaaa gagttcctga tgaaaaatct ccagcgttga cgctttactt
     1801 aatacaccgt ccagcggtga tggcgacaat gaaccaatca gccgagcttc aatatcatca
     1861 tccgcaataa ctaaacgccg ggtacgcgga aacttaatcg ccagttcagt caggcaagat
     1921 aatccctctc tgcgctcact tctcatggca gaaaaagaaa aatgaccgcc gcaaatgaga
     1981 tccgcgacat ggcatcctga aatgactcct gactggtata ggtatggagc ttataagggt
     2041 tacccgcaaa ctttctgaat aaactctcaa taccgatact gctcatgacg catttttcta
     2101 tgagtgcgac atttctcttc ttaattcggc tgtgttccat tctgcggcat ctccataaac
     2161 agaaggcaag ttaagttctc ttgcaccctg cgcgctattt atccatgcat acatttcagc
     2221 gttgctgtga attgccagtc gacgcatcgc actgtttttt tgggcgctga tagttttatt
     2281 acttttctta agcaacgaaa ctatctggtt gatgccccat cctttaccta atagccgcag
     2341 taccttgcgc tctgaaagag tgagaatgac ggagcgatag ctaaaatcat gagtttcatt
     2401 cattgccgga gaggtcagca tggcgctgat acgctctgcg tgcgtattgc cggaacgtac
     2461 ggtcttgacc agattctcaa tgggttcaac atcagacaat aacgtcgccg tagggcacat
     2521 gagcaattct actgcctggg aataccagga gcgtgacacc atgaaaaccc agtgaatttc
     2581 ccgatattgt gagattaaag aataataatg ttcacaaaca gaacgtggat cttcactttc
     2641 accggctaaa tcagcaatga ctaatccgga acgacgtaat tgtaataagg taaggtcctc
     2701 cgcagaggcg ctggatatta tttcatattc agggaagtga gtcctcatga cctcttttaa
     2761 ccctgcttgc ataacaggta ttttactgat aacaattcca tttttgcagc atcctggcaa
     2821 catatttcct ccacatccac tatctaaaaa tcatgatggt ttattcagga tattcattcc
     2881 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
     2941 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
     3001 atgataacct agtgaaatag cctggagaca tttaaaatat ctacattaag ttgttaggaa
     3061 tacttaatga aaaacagaaa tcacattcat ttaatttata attaaatgat atcccttccc
     3121 gcagaatgaa aagtcacaca accaatattt attcaatgaa atctgatggt tttaacttgt
     3181 taacattaaa aaacgtgccc ggttatctat tatcctgatt cgtaaaggcc aaacaataaa
     3241 ttcattatag tgataatatt catgtgatag tttgtttgcg gcgagagata attcgctttt
     3301 tatcaccgag cttgtcccga taaccaggat taatcagcgc aattttgatc ttaacctacc
     3361 gcaaaacaac cgtgcttcat gatacaaatg gattctcatt atctgaacaa tacgcagcac
     3421 gtctatgaca aagggcgagt tatgcaaact gagcaacagc gagccgtaac acggctttgt
     3481 atccagtgtg gattatttct tttgcaacat ggtgcggaaa gcgcgttggt tgatgagctt
     3541 tcctcacgac tgggtcgggc gttggggatg gacagcgtcg aaagttctat ctcttcgaac
     3601 gccatagtgc tgacaaccat taaagatggg caatgcctga catcgacacg taaaaatcac
     3661 gatcgcggca ttaatatgca tgtggtgact gaagtccagc acattgtgat tctggcggag
     3721 caccatctgc tggattacaa aggcgtagag aaacgattta gccaaattca gccattacgt
     3781 tacccaagat ggctggtagc cttaatggtt ggcctttctt gcgcctgttt ctgcaaactc
     3841 aataaaggtg gctgggatgg tgccatcatc accttctttg ccagtacggc cgcgatgtat
     3901 atccgccagc tgctggcaca acatcatctt catccgcaga tcaacttttg cctcaccgct
     3961 ttcgccgcca ccaccatttc cggattgctt ttgcaacttc ccactttcag caatacaccc
     4021 actatcgcga tggccgccag cgttctgctg ctggtgccgg gatttccgtt gattaatgcc
     4081 gtcgccgata tgtttaaagg ccacatcaat accggactgg cacgctgggc gatcgccagt
     4141 ctgctgacac tggctacctg cgtcggcgtg gtgatggcac tgacgatttg ggggctacgc
     4201 ggatgggtgt gatcgagttt ctgtttgcat tggcgcagga tatgatcctc gccgccattc
     4261 cagcggtcgg ctttgcgatg gtgttcaacg ttcccgttcg ggcattacgc tggtgtgcgc
     4321 tgcttggcgc gattggtcat ggttcacgaa tgatcttaat gaccagaggg ctgaatattg
     4381 agtggtcaac ctttatggcc tccatgctgg tcggcaccat tggtattcaa tggtcgcgct
     4441 ggtatctggc gcatccgaaa gtgtttaccg tggcggctgt tatccctatg ttcccgggca
     4501 tatcggctta taccgcaatg atttcggcgg taaaaatcag ccagttaggt tacagcgaac
     4561 cgttgatgat taccctgtta accaactttc ttacagcttc atcgattgtt ggtgcgttat
     4621 ccatcggtct ttccattcct ggattatggt tgtaccgcaa gcgccctcgc gtataaaatt
     4681 gcctcgctta aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     4741 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     4801 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     4861 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     4921 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga
     4981 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     5041 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     5101 gctatggggt gtggcactaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     5161 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     5221 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     5281 cagcgaacct gacagtcaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     5341 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     5401 ggtgaagaac tactggcctg gcagaaagaa caaggagcga tccgctccgc cgctctcgaa
     5461 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcggccactg
     5521 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     5581 agcaaagcgc gccagtacgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     5641 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     5701 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     5761 ttcaggaata gcggtaccag tgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     5821 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     5881 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     5941 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     6001 agtttgtggg tgatcttcaa ctgggatagc taccgtagcc gggtaacagg taaagagtat
     6061 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     6121 actgttaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     6181 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     6241 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcggg
     6301 aaccaggcct taagcgcaga taacatgaac aacgccgcag gcattctgca atactgcgcg
     6361 aagcaaaagc tggcgtcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     6421 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggatgg cattcagggt
     6481 ttgctgaaaa ccaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     6541 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     6601 tcctgaaccg cgaaacgccg cgttttagct gttacccggc tgacgcggct ctctcctgcc
     6661 cttttccctg cttctggacg gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa
     6721 agtctgttta taaactaatt cctaacaaca tcgcattttt ttgacactag aattgcagca
     6781 atatggcgac gccattacat tgttcttctc aaaaagctta aacgttggct aaacggcccg
     6841 gtttagtgag gatcgcccgt tgtcagaact actctctttc gccctttttc tcgcctctgt
     6901 gctgatttac gcatggaaag cgggacgtaa cacctggtgg tttgcagcca cattaacggt
     6961 gctggggcta tttgtcgttt taaatatcac cctgtttgcc agcgactatt ttactggcga
     7021 tggtattaac gacgcggttc tctatacctt aaccaacagc ctgaccggtg ctggcgtcag
     7081 caaatacatc ctgccgggta tcggcattgt gctggggctg acagcggtgt tcggtgcgct
     7141 gggctggatc ctgcgccatc gtcgccatca tccgcaccat tttggttaca gcctgctggc
     7201 gctcttactg gcgctgggtt cggtggacgc cagcccggca tttcgtcaga taacggaact
     7261 ggtgaaatcc cagtcacgcg atggcgaccc ggactttgcg gcttattata aagagccgtc
     7321 gaaaactatc cctgacccga aactcaacct ggtttatatc tacggcgaaa gtctcgagcg
     7381 gacctatttt gataacgaag ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa
     7441 tgaaggcctg gatttcagcc acacgcagca gctgccagga acggattaca cgattgcggg
     7501 catggtggct tctcagtgcg gcataccgct gtttgccccc tttgaaggca acgcctccgc
     7561 ctctgtctcc agcttcttcc cgcagaacat ctgtctgggc gatatcctga aaaactcggg
     7621 ttatcagaac tatttcgtgc agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct
     7681 gaagtcgcat ggcttcgacc acttatacgg ctcagaagag ctgaaaagcg tggtggccga
     7741 cccgcactat cgcaacgact ggggattcta cgacgatacc gttctcgatg aagcgtggaa
     7801 aaagtttgaa gagctttccc gctcaggtca gcgattctca ctgtttaccc tgacagtcga
     7861 tacccatcac ccggatggtt ttatctcgcg tacctgtaac cgcaaaaaat atgattttga
     7921 cggtaagccg aaccagtcat tcagcgcggt aagttgcagc caggagaata tcgcggcgtt
     7981 tatcaacaaa atcaaagcgt caccgtggtt taaagatacc gtcatcgtcg tctcttctga
     8041 ccatttagcg atgaacaaca cggcgtggaa atacctcaat aagcaggatc gcaataacct
     8101 gttttttgtc attcgtggcg acaagccgca gcaagagacg ctggcagtga agcgtaacac
     8161 gatggataac ggcgcgacgg tgctggacat tctcggtggc gataactatc tcggacttgg
     8221 tcgtagcagt ttatccgggc agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac
     8281 attggcgtgg aagccggata tcatccgcct gtggaaattc cctaaagaga tgaaagagtt
     8341 caccatcgac cagcagaaaa acatgattgc cttctcgggt agccatttcc gtttgccgct
     8401 gctgttgcgg gtttcagaca aacgcgtgga accgctgccg gaaagcgaat actcagcacc
     8461 gctgcgtttc cagctggccg atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg
     8521 ctataagatg gcacaactct gggctccgga actggcactc tccaccgact ggtgtgtctc
     8581 gcaagggcag cttggcggtc agcaaattgt tcaacatgtt gacaaaacaa catggcaggg
     8641 caaaacggca tttaaagata cggtcatcga catggcgcgt tataaaggca atgtcgatac
     8701 gctgaaaatt gttgataacg atattcgcta caaagccgac agtttcatct tcaacgtcgc
     8761 cggtgcgcca gaagaggtga aacagtttag tgggatttcc cgtccagagt cgtggggccg
     8821 ctggtccaac gcgcagctgg gcgatgaagt aaaaatcgag tacaagcatc cgctgccaaa
     8881 gaaatttgac ctggtgatta ccgccaaagc atacggcaat aacgccagcc gtcctattcc
     8941 ggtacgcgta ggcaatgaag aacaaaccct tgtgctgggc aatgaagtga ccaccaccac
     9001 actgcatttc gataacccaa ccgatgccga cacactggta attgtgccgc cggaacctgt
     9061 ctcaaccaac gaagggaata tcctcggaca ctcgccacgt aagctcggga tcggcatggt
     9121 ggaaattaaa gtggtggaac gtgaaggata attcctgact gatgtgtagg tcgcataagg
     9181 tgcgacctac acaccttctg cggcctacaa aacatcattg agaaacagaa aaaaacagcg
     9241 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     9301 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     9361 tggcgaacgg atagcgatgg gttaacatca tgtcagcagt gatttttcct tccgccatca
     9421 gtcgaccgac tttagcaaaa tcttccggcg tggcgttgcg gctacccatc atcgtcgttt
     9481 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacagg ccgacaaata
     9541 ccaccgtgcc gccgtgacga atcaaattca cggtgttatt catcgcatgt tgattacctg
     9601 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgtagc tgcgcgtcaa
     9661 aatcttcggc tgacgggtcc agtaaaggta attccagacg cgttgccaca tgttcacggc
     9721 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     9781 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     9841 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgctgcc gcctgcgggt
     9901 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     9961 catcctgatg cacgccaatg actgaaattt tttcacagca attggtacgc ccgcttttac
    10021 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgcaga ccatttttaa
    10081 gatcagcaat atttttaccc agcctaacaa tctccccaca tatttcatgg cctaaaacac
    10141 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
    10201 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
    10261 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
    10321 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
    10381 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
    10441 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
    10501 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
    10561 gtacaacatc agccgcacca cagtgcgtca tattctcagc catttacgcg aatgcggcgt
    10621 cttgacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccacg atgacggttt
    10681 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
    10741 gctgataaac cagcgccagt tacgcccagg ggaaactttt tctgaactgc aactggctcg
    10801 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
    10861 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
    10921 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
    10981 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctcgaac gtcatcggct
    11041 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
    11101 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
    11161 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
    11221 tgccgtcgat gagcatatga ccattctcag cgctctgatt tgccgcagtg acctcgatgc
    11281 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
    11341 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
    11401 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
    11461 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
    11521 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
    11581 tggaaaaaga aaatatcacc atcgatccgc gttcttcatt tactccatct tcgtcggcag
    11641 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
    11701 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
    11761 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccact gaaatcggcg
    11821 ctttgctctc cgtgttttca ctcgcttacg ggattgcgca acttccttgc ggcccactat
    11881 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
    11941 aggcaatgtc tggcatggtg cacaacttta cgcagttcgt gttggtgcgt atcggtatgg
    12001 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac
//
LOCUS       DAJTNN010000127        11772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTNN010000127 DAJTNN010000000
VERSION     DAJTNN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.127"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..898
                     /locus_tag="OO150_003014"
     CDS             <1..898
                     /locus_tag="OO150_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5517082.1"
                     /translation="VHINFPARKNWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCG
                     KSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRIITEPLWIAKR
                     SSEQQRRALVEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFFYERCPLATSG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(954..1385)
                     /gene="osmC"
                     /locus_tag="OO150_003015"
     CDS             complement(954..1385)
                     /gene="osmC"
                     /locus_tag="OO150_003015"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5517083.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            1731..1946
                     /gene="bdm"
                     /locus_tag="OO150_003016"
     CDS             1731..1946
                     /gene="bdm"
                     /locus_tag="OO150_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR5517084.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            2048..2185
                     /gene="sra"
                     /locus_tag="OO150_003017"
     CDS             2048..2185
                     /gene="sra"
                     /locus_tag="OO150_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR5517085.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            2342..4039
                     /gene="maeA"
                     /locus_tag="OO150_003018"
     CDS             2342..4039
                     /gene="maeA"
                     /locus_tag="OO150_003018"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5517086.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            4173..5183
                     /gene="adhP"
                     /locus_tag="OO150_003019"
                     /pseudo
     CDS             4173..5183
                     /gene="adhP"
                     /locus_tag="OO150_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            5329..5613
                     /locus_tag="OO150_003020"
     CDS             5329..5613
                     /locus_tag="OO150_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5517087.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(5664..6317)
                     /gene="fdnI"
                     /locus_tag="OO150_003021"
     CDS             complement(5664..6317)
                     /gene="fdnI"
                     /locus_tag="OO150_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5517088.1"
                     /translation="MSKSKMIVRTKFIDRVCHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(6310..7194)
                     /gene="fdnH"
                     /locus_tag="OO150_003022"
     CDS             complement(6310..7194)
                     /gene="fdnH"
                     /locus_tag="OO150_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5517089.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEAPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(7207..10254)
                     /gene="fdnG"
                     /locus_tag="OO150_003023"
     CDS             complement(7207..10254)
                     /gene="fdnG"
                     /locus_tag="OO150_003023"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(9667..9669),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5517090.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGERVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            10486..11367
                     /gene="yddG"
                     /locus_tag="OO150_003024"
     CDS             10486..11367
                     /gene="yddG"
                     /locus_tag="OO150_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5517091.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEAGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            11626..>11772
                     /locus_tag="OO150_003025"
     CDS             11626..>11772
                     /locus_tag="OO150_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5517092.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TNDKR"
BASE COUNT         2892 a         3039 c         2946 g         2895 t
ORIGIN      
        1 cgtccatatc aatttcccgg cccgtaaaaa ctggcttggt aaaactacgg aacatgttca
       61 tgcgattaat ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggcga
      121 gtcaggctgc ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca
      181 cgggcagtac atccgttctg gctcacaacg cattatgcag atggtgtttc aggacccgct
      241 ctcttcgctc aatccgcgct taccggtgtg gcgcatcatc acagaaccgc tctggatagc
      301 taagcgtagt agtgaacaac agcggcgagc gttggtagag gagctggctg tgcaggtggg
      361 tattcgtccg gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg
      421 tatcgccatt gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac
      481 ctctgcgctg gatatctccg tgcaggcgca gatcctcaat ttactggtaa cgctacagga
      541 aaatcacggg ctgacctatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag
      601 cgatcgggtg gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcaacaggt
      661 gctaacggct ccggcacatc catacacccg attattgctg gattccctcc ccgccattga
      721 taaaccgctg gaggaagaat gggcattacg taaaacggat ctgccgggaa accgcacgtt
      781 gccgcaaggc tgttttttct acgaacgttg cccccttgca accagcggat gtgaagtccg
      841 gcaatcatta gcaataaggg aggacggacg tgagctccgg tgctggcggg cgctgtagag
      901 atgctaacaa cgcatcaggc attcacgcgc ctgacacgtc atccggcaat gctttacgat
      961 ttcaactggt aatccagcgt aatttccgct ttcagtacct gagagaccgg gcatcctgct
     1021 tttgctttct ggatgatgcc gtcaaaggta gaggcatcaa tacccggcac cgcaacttca
     1081 ctcttcagtg cgattttcgt aatcgcaaaa ccggcatcca ctttatccag cgacacatcg
     1141 gcgatggtat caatcgatgt tggcgtaaat cccgcttccc ccagcatgaa tgaaagcgcc
     1201 attgagaaac atgcggcatg cgctgcgcca atcagttctt cagggttagt tcctttttcg
     1261 ccttcaaaac gcgtgttaaa tccatacggc tgttggttca gcacgccact ctcggtggat
     1321 actgttccct tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt
     1381 gtcattgttg ctctcatgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac
     1441 gaggataagc aggatattcc gaataaaaat ccggcagaaa tattacttct gcacttaaat
     1501 taaatttcca caatagataa ccgggaatat ataacccaag agatattgca ttaatattaa
     1561 tcaactcacc acaataataa tacctatcaa tatcatgagg ttaaaatata aaaatcatca
     1621 accaggacta atcttaacaa caaagcggca aatatttgcc atgctgaatg tgctttataa
     1681 atctgcgatc cgtagcagac accataaata cacagacacg gagaatcact atgtttactt
     1741 attatcaggc agaaaattca acagcagaac ccgctctggt taatgccatt gagcaaggcc
     1801 ttcgggcaga gcacggtgtt gtcactgaag atgacattct catggagctg accaaatggg
     1861 tggaagcttc tgataacgac atcctcagtg atatctacca gcaaacgatc aattatgtgg
     1921 tcagtggcca gcaccctacg ctttaaggtg ctatgcttga tcggcaacct aatttagggg
     1981 tttagcacgt gtttcttcgc tacggcgatg ttgtccttaa aactagcaac aggattgagg
     2041 agttaaaatg aaatcgaacc gtcaggcacg tcatattctt ggactggacc ataaaatttc
     2101 taaccagcgc aaaatagtta ccgaaggtga caaatccagc gtggtaaata acccaaccgg
     2161 cagaaaacgc cccgctgaaa agtaattcat aaccatcagt cctcaatgac gattaaacac
     2221 cattgcctgc gcaatggtgt ttttgttttt atctgcttta tacttggggc cgacgccctg
     2281 gcggtaaagc aaagacgata aaagcgtgcc agggatggat attcaaaaaa gagtgagtga
     2341 catggaacca aaaacaaaaa aacagcgttc gctttatatc ccttacgctg gccctgtact
     2401 gctggaattt ccgttgttga ataaaggcag tgccttcagc atggaagaac gccgtaactt
     2461 caacctgctg gggttactgc cggaagtggt cgaaaccatc gaagaacaag cggaacgagc
     2521 atggatccag tatcagggat tcaaaaccga aatcgacaaa cacatctacc tgcgtaacat
     2581 ccaggacacc aacgaaaccc tcttctaccg tctggtaaac aatcatcttg atgagatgat
     2641 gcctgttatt tataccccaa ccgtcggcgc agcctgtgag cgtttttctg agatctaccg
     2701 ccgttcacgc ggcgtgttta tctcttacca gaaccgccac aatatggacg atattctgca
     2761 aaacgtaccg aaccataata tcaaagtgat tgtggtgact gacggtgaac gtattctggg
     2821 gcttggtgac cagggcatcg gcgggatggg cattccgatc ggtaaactgt cgctctatac
     2881 cgcctgtggc ggcatcagcc cggcgtatac ccttccggtg gtgctggatg tcggaacgaa
     2941 caaccaacag ctgcttaacg atccgctgta tatgggctgg cgtaatccgc gtatcattga
     3001 cgacgaatac tatgaattcg ttgatgaatt tatccaggct gtgaaacaac gctggccaga
     3061 cgtgctgttg cagtttgaag actttgctca aaaaaatgcg atgccgttac ttaaccgcta
     3121 tcgcaatgaa atttgttctt ttaacgatga cattcagggc actgcggcgg taacagtcgg
     3181 cacactgatc gcagcaagcc gagcggcagg tggtcagtta agcgagaaaa aaatcgtctt
     3241 ccttggtgca ggttcagcgg gatgcggcat tgccgaaatg atcatcgccc agacccagcg
     3301 cgaaggatta agcgaggaag cggcgcggca gaaagtcttt atggtcgatc gctttggcct
     3361 gctgacggac aagatgccga acctgctgcc tttccagacc aaactggtgc agaaacgcga
     3421 aaacctcagt gactgggata ccgacagcga tgtgctgtca ctgctggatg tggtgcgcaa
     3481 tgtaaaacca gatattctga ttggcgtctc aggacagacc gggctgttta cggaagagat
     3541 catccgtgag atgcataaac actgtccgcg tccgatcgtg atgccgctgt ctaacccgac
     3601 gtcacgcgtg gaagccacac cgcaggacat tatcgcctgg accgaaggta acgcgctggt
     3661 cgccactggc agcccgttta atccagtggt atggaaagat aaaatctacc ctatcgccca
     3721 gtgtaacaac gcctttattt tccctggcat cgggctgggt gttattgctt ccggcgcgtc
     3781 acgtatcacc gatgagatgc tgatgtcagc aagtgaaacg cttgctcagt attcgccgct
     3841 ggtgctgaac ggcgaaggtc tggtactacc ggaactgaaa gatattcaga aagtctcccg
     3901 cgcaattgcg tttgcggttg gcaaaatggc gcagcagcaa ggcgtggcgg tgaaaacgtc
     3961 tgctgaagct ttgcaacaag ccattgacga taatttctgg caagccgaat accgcgacta
     4021 ccgccgtacc tccatctaag cctgcgcccg gtagtgaagg ctaccgggct atttccctct
     4081 ccctttttca gatctcatcc atactgggta gtggcgaata aatctcattt gcctcacctg
     4141 ctatgcagaa catcatccga aaaggaggaa ctatgaaggc tgcagttgtt acgaaggatc
     4201 atcatgttga cgttacggat aaaacactgc gctcactgaa acatggcgaa gccctgctga
     4261 aaatggagtg ttgtggtgta tgtcataccg atcttcatgt taagaatggc gattttggtg
     4321 acaaaactgg ggtaattctg ggccatgaag gcatcggtgt ggtggcagaa gttggcccag
     4381 gtgtcacctc attaaaacca ggcgatcgtg ccagcgtggc gtggttttac gaaggatgcg
     4441 gtcattgcga atactgtaac agtggtaacg aaacgctctg ccgttcagtt aaaaatgccg
     4501 gatacagcgt tgatggcggg atggcggaag agtgcatcgt ggtcgccgat tacgcggtaa
     4561 aagtgccaga tggtctggac tcggcggcgg ccagcagcat tacctgtgcg ggggtcacca
     4621 cctacaaagc cgttaaactg tcaaaaattc gtcccggtca gtggattgct atctacggtc
     4681 ttggcggtct gggtaacctc gccctgcaat acgcgaagaa tgtctttaac gccaaagtga
     4741 tcgccattga tgtcaatgat gagcagttaa aactggcaac cgaaatgggt gcagatttag
     4801 cgattaactc acgcaccgaa gacgccgcct aaattgtgca ggagaaaacc ggtggcgctc
     4861 acgctgcggt ggtaacagca gtagctaaag ctgcgtttaa ctcggcagtt gatgctgtcc
     4921 gtgcaggcgg tcgtgttgtg gctgtcggtc tgccgccgga gtctatgagc ctggatatcc
     4981 cacgtcttgt gctggatggc attgaagtgg tcggttcgct ggtcggcacg cgccaggatc
     5041 taaccgaagc cttccagttt gccgccgaag gcaaagtggt gccgaaagtc gccctgcgtc
     5101 cgttagcgga catcaacacc atctttaccg agatggaaga aggcaaaatc cgtggccgta
     5161 tggtgattga tttccgtcac taagaggtct ttgctgcgac tcccatgttc gggtcgcagc
     5221 atcgcacact ctccaacatg aaatggctaa atggattatt gttgcgtagg aaggaacaat
     5281 aatgatagtg ttcccagaga aggaacgcta ctgagagtaa agacactaat gaaaatggcc
     5341 aatcatcccc gcccggggga cattattcag gaatcactgg acgaacttaa tgtcagcctg
     5401 cgcgagtttg ccagagcaat ggaaattgcg ccctcaacgg caagccgatt gctgaccgga
     5461 aaagcagctt tgacgccaga aatggcaatt aaactttccg tggtgatcgg cagttcgccg
     5521 caaatgtggc tgaatctgca aaacgcctgg agtctggcag aggctgaaaa aacggtggat
     5581 gtgtcgcgac tgcgccgtct ggttacgcaa taaaaaaatg gcgcttctta aagcgccatt
     5641 ttcagcttaa agttcaaaag agattatatc ccttcttcac tctctttttt cgcttctgcc
     5701 ttctcgattt cacgatacca gcgcggatgg tgtttcttcg cccagcgacg gcttaccttc
     5761 ccttcgatca tccctttaat cgatcctttc acccagaatg ccatatacat atggatcagg
     5821 atggcgtgga tcaggatgat accggcagcc gcgtggatca gcaggctgta gcgaacaacc
     5881 tgcatcggga agtactgcgc aaagtacgga cgccagataa tcaccccggt caccaacagc
     5941 acgaaaatca tgctcatgat cgaccagaac atcatctttt gcccggcgtt gtacttacca
     6001 acatccgcca ctttgtgctc attgcctttt aatacttcga caatgttcaa tagccacgga
     6061 atatctttct tatccgggat gttgtgatgc acaaaacgca caaacataaa catcagcgcg
     6121 acgaaaatcg caatgccgaa gaacgggtgc aaaatgcgtc ccatctgcgg cgtaccgaag
     6181 gtttgcgtca gccattgcag cgtcgggaag aagaacgaaa tcccggacag cgccaccagg
     6241 aagaagcaaa tcaccacggt ccagtgacag acgcgatcaa taaatttggt gcgcacaatc
     6301 attttcgact tactcatgat gatcctcctc gtcatcgtcc acttccttat tcgggccaat
     6361 accgatgtag tggaaaatca acccggcaaa agtggcaata aagccagccg ctgccagcgg
     6421 tttcaacgcg cctttccaca ggcttaccga ggtgtcgatc ttcggatctt tcggcagacc
     6481 gtgatacagc tccggctgat cggcgtgatg cagcacgtac ataacgtgcg taccaccgac
     6541 cccttccggg ttgtagacgc cagcatgttc gtaaccacgc gctttcagtt tcgccacgcg
     6601 ctgttccgcc agctccagca tctccttctt ggtgccgaag tggatagccc cggtcggaca
     6661 ggttttcaca caagccggtt cctggccgac gctgacgcga tcgacgcaga gcgtgcattt
     6721 atatacccgg ttatcctctt tgttgaggcg cggaatatta aacggacacc cggcaatgca
     6781 gtaaccacag ccgatgcagt tttccgactg gaaatcgaca atcccgttag cgtactgaat
     6841 gattgcacca gcagacgggc acgccttcag gcagccggga gcttcacagt gcatacagcc
     6901 gtctttacgg atcagccact ccagcttgcc gttctgttcg gtttcgctaa agcgcatcac
     6961 cgtccaggac ttggcgctca gatcggcggg gttatcgtaa accccgacgc agtgccccac
     7021 ttcatcacgg atgtcgttcc actccgaaca cgccacctga caggctttac agccgataca
     7081 ggtggaaacg tcgataagtt ttgcgacttc tgctttgtaa tcacgcacct gagaaggcgg
     7141 cgtgatggag ttagttgcgg accttttgat aatgtcctgc gtttccatag ccatctgttc
     7201 gcccccttac gccttctcga tgttgactaa gaacgcttta tattccggcg tttgcgagtt
     7261 tgcatcaccg acattcggcg tcagagtgtt agcgatataa cctttacgcg cgacaccctc
     7321 aaagccccag tggattggaa tacccaccgt ttcaacctgc tgaccattta cattcagcgg
     7381 tttcagacga cgcgttacca cagccaccgc gcggataaag ccacgcttgc tggagacagt
     7441 gacacgctcg ccattattaa tgcctttcgc cgccgccagc gtttcgctga tttccacaaa
     7501 ctgttccggc tgagcaattg cgttgagcaa tgcgtgcttg gtccaggtgt ggaagtgctc
     7561 ggtcagacga taggtcgtac ccacatacgg gaactgctct tttttaccca tccgcagcgc
     7621 gtcttgttca tacagacgaa caaccgggtt agacaccacg ttcggatgca gcgggttagt
     7681 gcccagcggc gtttcaatcg gctcgtagtg ttccgggaac ggaccttccg ccattttgtt
     7741 gatggcaaac aggcgtccca tcccttccgg ctgcatgata aacggcccgg ttggcgtacc
     7801 cggtgcggca ttgccgaagt caggaatatc gttacccgtc cacttgctgc cgttccactg
     7861 gatcagcatc cgtttcggat cccacggttt accgttgata tccgccgaag cacggttgta
     7921 cagcacgcga cggttgagcg gccacgccca ggcccatccc agcgtattcc ccagaccgga
     7981 cgggtcggag ttatcgcggt tagccatctg gttgccctgc tctgtccagc taccggtgta
     8041 gatccagcaa gaagatgcgg ttgtaccgtc atcacgcaga tgcgcaaagc tactcagcaa
     8101 ctgacctttc ttcgcaataa gcacgccatt agcgtcatag agatcttcca gcgcgtagcc
     8161 gttgttctct ttagccactt cgtcagattg cggttcgtgc ggctgcttgt agttccagct
     8221 catcttcatc agcggttcta cgcctttacc accttcggcc tggtacagct cgcgcagatg
     8281 atggtagata cccgccagaa tttcgccgtc gttacgcgct tcgcccggcg cgtcctgacc
     8341 tttccagtgc cactgcaacc agcgaccgga gttagcgata gaaccatctt cttcggcaaa
     8401 gcaggtcgaa ggcagacgga atacttcagt ctgaatagac gccggatcga catcgttcga
     8461 ctcgccgtgg ttctgccaga aggtagaggt ttcagtcacc agcggatcga taaccaccat
     8521 gtacttcagc ttgctcaggc agctcaccac tttgtttttg tccgggaagg acgcaaccgg
     8581 gttaaagccc tggcagaaat aaccggtgac tttgccttcg tccatcatgt tgaaatactt
     8641 gatgacgtcg taggtctggt cccacttcgg cagccagtca tagccccagt tgttctcttt
     8701 ctgcgcggca tcgccataga aagatttcat caggctaacg aagaacttcg gatagttgct
     8761 ccagtagttc acctgaccag ccagcgtcgc tttcggcgtg ttcgcttcca gatacgactg
     8821 caaatcaacc tgtttttctg acggaagcgt cagataacct ggcaggctgg tagagagcag
     8881 acctaagtca gtcagcccct gaatgttgga gtgaccacgc aatgcgttca cgccgccacc
     8941 ggccataccc atgttaccga gcagcaactg gatcatcgcc atagtacgga tgttctgcgc
     9001 acccacggtg tgctgcgtcc agcccagcgc gtacaggaag gtggttgtgc gatccggtgc
     9061 gctggtggag gccagcactt cacacacttt caggaagtcg gcttttggcg taccgcagat
     9121 gttttcaacg acgtccggcg tgtagcggga aacgtgcgct ttcagcaggt tccacacaca
     9181 gcgcggatga gtcagtgttt catcgcgttt tgcatagccg ttttcatcga actgatagtt
     9241 ccaggacgac ttatcatact ggcgtttttc agcgtcgtag ccgctgaaca ggccgtcttc
     9301 gaaagcaaaa tcatcacgca ccagcaggct ggcgttggtg taatgtttaa cgtattcggc
     9361 gttgattttg ttgttttcga tcaggtagcg caaaacgcca gacaggaacg taatgtccgt
     9421 accggaacga ataggtgcgt aaatatccgc cacagaagcg gtacgcgtaa aacgaggatc
     9481 gacagcgatc aaggttgcat cgttgttgtt tttcgcttcc atcgcccagc ggaaaccgac
     9541 gggatgcgct tcagcagcgt taccgcccat taccattacg acgttagcgt ttttgatatc
     9601 cacccagtgg ttggtcatcg caccgcgacc aaatgttgga gcaagacttg ctaccgttgg
     9661 tccgtgtcag acgcgcgcct ggttgtctac cgccagcatc ccgagggagc gggcaaattt
     9721 ctgtgtcagc atcccggttt cgttgctggc accggaggca cacagcatac cggtagaaag
     9781 ccaacggttt accgttacgc cctgctcgtt cttttcaata aagttagcgt cacggtcagc
     9841 tttcatcagc tttgcaatac gggagaatgc ttcttcccag ctaatgcgct gccatttgtc
     9901 agaacctggc gcacgatatt ccgggtagcg cagacggttt tcgctgttga cgtaatccag
     9961 caaaccggcc ccttttgggc acagcgcacc acggcttacc ggatgatccg ggtcaccttc
    10021 aatgtgataa atcgcttctc tggcgttttt cgcgccatca cccaggctat acatcaatag
    10081 cccgcaacct acggaacagt atgtgcaggt gttacggatc tctttagcgc gtaataattt
    10141 gtagtttcgc gcctgagcca gtgcttgctt aggggcaaag cccaatgccg ctaccgttgt
    10201 tccagccata ccgcccgcgc agattttaaa aaattgtctg cgactgacgt ccattgcttt
    10261 cctctttttt cagggatatt actgcgcgag gaaactaaca gtaaaattct gaatcttttt
    10321 gcgcgaaatc aaaaagaaca cgtgtttgcc acaaaataat ccgcctgcgc aaattgacct
    10381 acctcaatag cggtagaaaa acgcaccact gcctgacagg ccagttaaaa aaatgctata
    10441 aaattcagct taatttttaa cggcaagaga gacaaaacag cgagcatgac acgacaaaaa
    10501 gcaacgctca tagggctaat agcgatcgtc ctgtggagca cgatggtagg attgattcgc
    10561 ggtgtcagcg aggggctcgg cccggtcggc ggcgcagctg ctatctattc attaagcggg
    10621 ctgctgttaa tcttcacggt tggatttccg cgtattcggc agattccgaa aggctattta
    10681 ctcgccggga gtctgttatt cgtcagctat gaaatctgtc tggcgctttc cttagggtat
    10741 gcggcgaccc gtcatcaggc gattgaagcg ggtatggtga actatctgtg gcccagcctg
    10801 acaattctct ttgccattct gtttaatggt cagaaaacca actggctgat tgtaccagga
    10861 ttattattag ccctggtcgg cgtctgctgg gtgttaggcg gtgagaatgg gttacattat
    10921 gatgaaatca tcaataatat caccaccagc ccattgagtt atttcctggc gtttattggt
    10981 gcgtttatct gggcagccta ttgcacagta acgaataaat acgcacgcgg atttaacgga
    11041 attaccgttt ttgtcctgct aacgggagca agtctgtggg tttactattt tcttacgcaa
    11101 caaccagaaa tggtatttag cacgcccgtc atgattaaac tcatctctgc ggcatttacc
    11161 ttaggatttg cttatgctgc atggaatgtc ggtatattgc atggcaatgt caccataatg
    11221 gcggtaggct cgtattttac gcccgtactt tcctcagcgc ttgcagccgt gctgctcagc
    11281 gccccgctgt cgttctcatt ctggcaaggc gcgctaatgg tctgcggcgg ttccctgctc
    11341 tgctggctgg cgacacgtcg tggttaaaat aattgccggg tctacggccc ggcagttaat
    11401 cagataaatt ccgacaatat taaaaaataa atttcaaata ccagtaattc accttatatc
    11461 tcatttcgac ttcgatcaca tatcgataaa taatattatc tcatctattt ttctcattgt
    11521 ttttaatcac cattctattt taaaaacagt atgtcattta taaatgacgc cgtttaaaat
    11581 tcgtttagaa aatacgtttg acgataatta aaaggattct gttaaatgaa attaaaaata
    11641 gttgcggtgg ttgtaactgg tttgttagct gcgaacgtag cacacgctgc cgaagtctat
    11701 aacaaggatg gtaataaact cgacctttat ggcaaggtta ccgctctacg ttattttact
    11761 aatgataagc gt
//
LOCUS       DAJTNN010000128        11770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTNN010000128 DAJTNN010000000
VERSION     DAJTNN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.128"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..227)
                     /locus_tag="OO150_003026"
     CDS             complement(<1..227)
                     /locus_tag="OO150_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5517093.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYVLTTQVEFTDPEIVAEYITYP"
     gene            383..1423
                     /gene="gpr"
                     /locus_tag="OO150_003027"
     CDS             383..1423
                     /gene="gpr"
                     /locus_tag="OO150_003027"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5517094.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1463..1957)
                     /locus_tag="OO150_003028"
     CDS             complement(1463..1957)
                     /locus_tag="OO150_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5517095.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2148..3032
                     /gene="yghA"
                     /locus_tag="OO150_003029"
     CDS             2148..3032
                     /gene="yghA"
                     /locus_tag="OO150_003029"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5517096.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3304..3729)
                     /gene="exbD"
                     /locus_tag="OO150_003030"
     CDS             complement(3304..3729)
                     /gene="exbD"
                     /locus_tag="OO150_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5517097.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3736..4470)
                     /gene="exbB"
                     /locus_tag="OO150_003031"
     CDS             complement(3736..4470)
                     /gene="exbB"
                     /locus_tag="OO150_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5517098.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4722..5909
                     /gene="metC"
                     /locus_tag="OO150_003032"
     CDS             4722..5909
                     /gene="metC"
                     /locus_tag="OO150_003032"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5517099.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6049..6708
                     /locus_tag="OO150_003033"
     CDS             6049..6708
                     /locus_tag="OO150_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5517100.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6748..7647)
                     /gene="yqhC"
                     /locus_tag="OO150_003034"
     CDS             complement(6748..7647)
                     /gene="yqhC"
                     /locus_tag="OO150_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5517101.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            7841..9004
                     /gene="yqhD"
                     /locus_tag="OO150_003035"
     CDS             7841..9004
                     /gene="yqhD"
                     /locus_tag="OO150_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5517102.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAADNYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9109..9936
                     /gene="dkgA"
                     /locus_tag="OO150_003036"
     CDS             9109..9936
                     /gene="dkgA"
                     /locus_tag="OO150_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5517103.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            10136..11062
                     /locus_tag="OO150_003037"
     CDS             10136..11062
                     /locus_tag="OO150_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5517104.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11113..11370
                     /gene="yqhH"
                     /locus_tag="OO150_003038"
     CDS             11113..11370
                     /gene="yqhH"
                     /locus_tag="OO150_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5517105.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
BASE COUNT         3054 a         2976 c         2939 g         2801 t
ORIGIN      
        1 ggatacgtga tgtactcagc aacaatctcc gggtcggtaa actctacctg agtcgtcagc
       61 acataatttg gcttgagcaa atcaaacaac gctaatgccg tcatcccgcc caccgcatac
      121 ttcgccgcaa ggttgaggaa ctcacgtttc gaaattttcc cgtgagcgta atagtcgtag
      181 taatccaaca actcttgtgg gaaatctttg gcggttaaac gcggcatcgt tgcattcctc
      241 aactggtgtt ttttaagcaa agcataagcg cgtgtttttg cccagttttt cgtcactctg
      301 tgagccagac tacgggatac gcgctggcga atcgctaaac tagaaacatt gtttcgaaat
      361 tgaacggtgg aaaggagagg tcatggtctg gttagcgaat cccgaacgtt acgggcagat
      421 gcagtaccgc tattgcggaa aaagtggttt acgcctgccc gcgttatcgc tcggtttatg
      481 gcacaatttc ggtcacgtta acgcgctgga atcacagcgt gcaatcctgc gtaaagcgtt
      541 tgatttaggc attacgcact ttgatttagc caacaattac gggccgcctc caggaagcgc
      601 agaagagaac tttggtcgcc tgctgcggga ggattttgcc gcttatcgcg atgaactgat
      661 tatctctacc aaggctggct acgatatgtg gcccggccct tacggctctg gcggttcacg
      721 taaatacctg ctcgccagcc tcgaccaaag cctgaagcgt atggggcttg agtatgtcga
      781 tatcttttac tctcatcgcg tcgatgaaaa tacgccgatg gaagaaaccg cctctgcgct
      841 ggctcatgcg gtacaaagcg gtaaggcgct gtatgtcggg atctcctctt actcgccaga
      901 gcggacgcaa aaaatggtcg agttgctgca cgagtggaaa attccgcttt taattcatca
      961 accttcgtac aatttactga accgctgggt ggataaaagc ggcctgctgg ataccctgca
     1021 aaataacggc gtgggctgta ttgcctttac tcctctggct cagggattgc tgaccggaaa
     1081 atatctcaac ggcattccgc aagattcacg gatgcatcgt gaagggaata aagttcgtgg
     1141 tctgacgccg aaaatgctta ccgaagccaa cctcaacagc ctgcgcttat tgaatgaaat
     1201 ggcacagcag cgtggacaat caatggcgca aatggcgtta agctggttgc tgaaagatga
     1261 tcgcgtgacg tcggtattga ttggtgtcag ccgcgcggag caactagagg agaacgtgca
     1321 ggcgctgaat aatctgacat ttagcaccga ggagctggcg cagattgatc agcatatcgc
     1381 cgatggcgag ctgaatctgt ggcaggcgtc ttccgataaa tgacctgtta ataacgggcg
     1441 cgcgaaccgc gcccgaatca gatcagtgat tatgacgagt cagtcggtca agatagccca
     1501 tcacaaatgc agagagcaca aacgtcagat ggataatgac gtaccacatc agtttgttat
     1561 ccgggacatt tttcgcatcc ataaagacgc gcagtaagtg aatggaagaa attgccacaa
     1621 tcgacgctgc tactttgttt ttcagcgacg ttgcgtccat tttccccagc cagttcagct
     1681 tctctttgtt ctcggagata tccagctgcg agacgaaatt ctcataaccg gaaaacatca
     1741 ccatcaccag caaaccgcca accagcgtca tatccaccag cgacagcaac acgaggatca
     1801 aatctgattc cgccatcgag aagatattcg gcagtacgtg aataatctcc tggaagaact
     1861 tcagcgccag ggcaactaac gccagcgaaa ggccaaagta cacgggggca agcagccagc
     1921 gagaagcata cattgcattt tcaagaaaac gttccataca ttcctgtcgt gtagttaaaa
     1981 cgggcaacag tatatcgcaa ttgagtaaac agcacgcaac agcaaatcaa catatacgtt
     2041 aaacctttgt cgcataattc tgaggtttag ggatcttcct ggttccctta tcaacgcatc
     2101 gagagcgtct acccttaaaa gacgttgaac ctgaagggag aaaaacaatg tctcatttaa
     2161 aagacccgac tacgcagtat tacactggtg aatatcccaa acagaaacaa ccgacgccag
     2221 gcatccaggc gaagatgaca ccggtaccgg attgcggcga gaaaacctat gttggtagcg
     2281 gtcgcctgaa agatcgtaaa gcactggtga cagggggcga ttccggaata ggtcgcgctg
     2341 ccgccatcgc ttacgcgcgt gaaggggctg acgtggcgat cagttatctt cccgtggaag
     2401 aagaagacgc tcaggatgtg aaaaagatca ttgaagaatg cggacgcaaa gccgttctgc
     2461 tgccaggcga tttaagcgat gagaaattcg cccgttcgct ggttcacgaa gcgcacaagg
     2521 cgttaggcgg gctggatatt atggcgctgg tcgccgggaa acaggttgcc attccggata
     2581 ttgcagacct caccagcgaa cagtttcaaa agacctttgc cattaacgtt ttcgcgctgt
     2641 tctggctaac ccaggaagcg atccccctgc taccgaaagg tgcaagtatc atcaccactt
     2701 cgtcaatcca ggcataccag ccaagtccgc atttactgga ctatgcggct acgaaggcgg
     2761 cgattctgaa ctacagccgt ggcctggcaa aacaggtcgc ggagaaaggt attcgggtga
     2821 atattgtcgc gccaggccct atctggacgg cactgcaaat ttccggcgga caaacgcagg
     2881 ataagatccc gcagtttggt cagcaaacgc cgatgaagcg tgcggggcaa ccggcggaac
     2941 tggcccctgt atatgtttat ctggcaagtc aggagtcgag ctacgtcacc gcagaagtgc
     3001 acggtgtgtg cggcggcgag catttaggtt aaaaaaatgc ccggttgtga aaagcaaccg
     3061 ggcatcattg tgaattcact cttacctgat gtggcgaaaa cgccttatcc gcctacaaac
     3121 gcatgaagat tcaacatgtt gcaatagatt ttgtaggcct gataagcgta gcgcatcagg
     3181 cattgagcac ctaatgccgg atgcggcgtg aacgccttat ccggcctaca aacgcacgaa
     3241 gattcaacat gttgcaatag attttgtagg cctgatgagc gtagcgcatc aggcaattct
     3301 actttacttc gctttggcgg tttcttcgcc gaccagacct atcttcaggt aacccgcctg
     3361 atgcagcgta tccattacct tcatcaacgt ctcgtaatcg acggttttat ccgctcggaa
     3421 gaagatggtg gtgtctttct tgccttcggt taacgcattc aacgccgtaa tcattgtttc
     3481 atcggtgacc ggatcgttac cgataaacat cgagttgtct gccttcaccg acagataaac
     3541 cggtttttcc gggcgcggct gcggcgtgct ggtagaagca ggcaagttca ccttcacatc
     3601 taccgtcgct aacggtgccg ccaccataaa gataatcagc agaaccaaca tcacgtcgat
     3661 aaacggcgtc acgttgatat catgcatttc gccgttatcg tcgaggtttt cgttaagatg
     3721 cattgccatc ggatattatc ctgcgcgtaa tttttgtgcg acacgaaccg gatgcgcagc
     3781 ggcgctggct tccagatcca ggtcacggct ttgcagcaac aatacctgcg ctgcaacatc
     3841 acccagcatc gctttaaagc cgccaatctg gcgtgcaaat acgttataga taacgaccgc
     3901 aggaatcgct gccacgaggc cgattgccgt tgctaacaga gcttctgcga tacccggcgc
     3961 aacgactgcc aggttagtgg tctgcgtttg cgcgatacca ataaagctgt tcatgatgcc
     4021 ccagaccgta ccaaacagac caacgaacgg agaaatcgcg ccgatggttg ccagatagcc
     4081 gttaccgcgg cccatttgac gacccactgc ggcgacccga cgctccagac ggaagctggt
     4141 acgttcttta ataccttcgt tatcgtcgct gccttctgac agttccagct cattctgcgc
     4201 ttcattgaac aaatgcaggc ttaagctttt gctaccaaaa tcagcggcga tatcgttggc
     4261 ctggtttaag gaacgcgctt cagccagcag ttgctgctcg cgctttaggc gacgcttctg
     4321 attgaagaac tctacgctct tactgaagaa gattgcccag gtgactacgg aggccaaaat
     4381 aagcccaatc atcacgcact taacgacaat atcggcgtgc tgatacatac cccagacgga
     4441 aaggtctgtc tgcattaaat tattacccac tgtgtatctc caggacgcaa gtcacaaaat
     4501 ctgcgcataa taatatcaaa acgacgtcga attgatagtc attctcatta ctatttgcat
     4561 actgccgtac ctttgctttc ttttccttgc gtttacgcag taaaaaagtc accagcacgc
     4621 catttgcgaa aattttctgc tttatgccaa ttcttcagga tgcgcccgcg aatattcatg
     4681 ctagtttaga catccagacg tataaaaaca ggaatcccga catggcggac aaaaagcttg
     4741 atactcaact ggtgaatgca ggacgcagca aaaaatacac tcttggcgcg gtaaatagcg
     4801 tgattcagcg cgcttcttcg ctggtatttg agagtatgga agccaaaaag cacgcgacgc
     4861 gcaatcgcgc caatggcgag ttgttctatg gacggcgcgg aacgttaacc catttttcct
     4921 tacaacaagc gatgtgtgaa ctggaaggtg gcgcaggctg cgcgctattt ccctgcgggg
     4981 cggcagcggt tgctaattcg attcttgctt ttgtcgaaca gggcgatcat gtgttgatga
     5041 ccaacactgc ctatgaaccg agtcaggatt tctgtagcaa aatcctcagc aaactgggcg
     5101 taacgacatc gtggtttgat ccgctgattg gtgccgatat cgttaagcat ctgcagccaa
     5161 acactaaaat cgtgtttctg gaatcgccag gctccatcac catggaagtc cacgacgttc
     5221 cggcgattgt tgccgccgta cgcagtgtgg tgccggatgc catcattatg atcgacaaca
     5281 cctgggcagc cggtgtgctg tttaaggcac tggattttgg catcgatgtt tctattcaag
     5341 ccgccaccaa atatctggtt gggcattcag atgcgatgat tggcactgcc gtgtgcaatg
     5401 cccgttgctg ggagcagcta cgggaaaacg cctatctgat gggccagatg gtcgatgccg
     5461 ataccgccta tataaccagc cgtggcctgc gcacattagg tgtgcgtttg cgtcaacatc
     5521 atgaaagcag tctgaaagtg gctgaatggc tggcagaaca tccgcaagta gcgcgagtta
     5581 accaccctgc tctgcctggc agtaaaggac acgaattctg gaaacgagac tttacaggta
     5641 gcagcgggct gttttccttt gtgcttaaga aaaaactcag taatgaagag ctggcgaact
     5701 atctggataa cttcagttta ttcagcatgg cctactcgtg gggcgggtat gaatcgttga
     5761 tcctggcgaa tcaaccagaa catatcgccg ccattcgccc acaaggcgag atcgatttta
     5821 gcgggacctt gattcgcctg catattggtc tggaagatgt cgacgatctg attgccgatc
     5881 tggacgccgg ttttgcgcga attgtataac attgccactt ttggacaatt ttgcagacat
     5941 ttaattgtga aaagtcttaa attgttgcgt ccgggatcaa ggcgtcccgg acgaatcagg
     6001 agtacaatag gcagataaag gcttaaacgc tgttccacag gaaagtccat ggctgttatt
     6061 caagatatca tcgctgcgct ctggcaacac gactttgccg cgctggcgga tcctcatatt
     6121 gttagcgttg tttactttgt catgtttgcc acgctgtttt tagaaaacgg cctgctgccc
     6181 gcctcatttt tgccaggcga cagcttgttg atactggcag gcgcattgat tgcccagggg
     6241 gttatggatt ttctgcctac gattgcgatt ctgaccgccg cagcaagtct gggctgctgg
     6301 ctaagttata ttcaggggcg ctggttaggg aataccaaaa cggtgaaagg ctggctggca
     6361 cagcttcctg ctaaatatca ccagcgcgcc acctgcatgt ttgaccgcca cggtctgctg
     6421 gcgctgctgg ctggacgttt tcttgcattt gtccgtacgc tgctgccaac catggcggga
     6481 atttccggtc taccaaaccg ccgcttccag tttttcaact ggttaagtgg attgctgtgg
     6541 gtcagcgtgg taaccagttt tggctatgcc ttaagtatga ttccgttcgt taaacgccat
     6601 gaagatcagg taatgacgtt cctgatgatc ctgccaattg ccttgttaac cgctggcttg
     6661 ttaggcatgc tgtttgtggt gattaaaaaa aaatactgta acgcctgacg attttccccg
     6721 ttcccggctg ctgtaccggg aacgtattta attcccctgc atcgcccgca ttcttgccgc
     6781 atcttccccc ggcgtcacac cgaagtaacg tttaaactca cggctaaatt gcgatgcgct
     6841 ttcatagccg acgcgcatcg ctgctgcgct ggccttcatg ccgtcatgga tgatcatcat
     6901 ccgcgcctta tgcagacggt aattcttcaa atactgcaac ggcgaggtgc tggtgacaga
     6961 cttaaaatta tggtggaacg ccgatacgct catgttggct tctgccgcca gttgctcgac
     7021 gctcaggttt tcggtgtatt tattctcaat ccgtttcagc acgcggctaa tcagactgaa
     7081 gtgagtctgg cgactgacca gcgccagtaa cgcgccgccg caaggtccgg tcagcacgta
     7141 gtacagaatt tcgcggatga tctgtttgcc gagaatacgc gcatccagtg gtcgctccat
     7201 cacgtcgagt aaccgctccg ccgcgcataa aatctcttct gataacgtgg cggagttaat
     7261 cccgctggct gccatcgacg gctggaaatg ctcatcttcg ccaatgtcca tcaacagttc
     7321 ctgtaactgc aaaatatcga cattgagacg caaccctgcc agcggcacct ctgacgtggc
     7381 ataggtttcg cactcaaacg gcaacggcac cgtcagcagc aggtattcat tggcatcata
     7441 acgaaacacg cgttcattga tataaccgat tttatgcccg gaaaagagaa ttatgatgcc
     7501 aggctcgtac atcaccggtg tacgtgcgaa aggcgtctca ccatacaaca aacgcacatc
     7561 gggcaacagt tctgacaaac tattttcttt atttttcagt ttattaactt tatccgccag
     7621 caagcggcaa atctcttcac gtttcatatc gcgtaatttc ttaggaataa tgcggcaatt
     7681 tgattgtgcg caattttgta gcatttctcc agcactctgg agaaataggc aagacattgg
     7741 cagaaatgag cattgagagc cagggcgctg gcgatcacaa tgaaaaacat caggcagatc
     7801 gttctctgcc ctcatattgg cccagcaaag ggagcaagta atgaacaact ttaatctgca
     7861 caccccaacc cgcattctgt ttggtaaagg cgcaatcgct ggtttacgcg aacaaattcc
     7921 tcacgatgct cgcgtattga ttacctacgg tggcggcagc gtgaaaaaaa ccggcgttct
     7981 cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg gaatttggcg gtattgagcc
     8041 aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg gttcgcgaac agaaagtgac
     8101 tttcctgctg gcggttggcg gcggttctgt actggacggc accaaattta tcgccgcagc
     8161 ggataactat ccggaaaata tcgatccgtg gcacattctg caaacgggcg gtaaagagat
     8221 taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca gcaaccggtt cagaatccaa
     8281 cgcaggcgcg gtgatctccc gtaaaaccac tggcgacaag caggcgttcc attctgccca
     8341 tgttcagccg gtatttgccg tgctcgatcc ggtttatacc tacaccctgc cgccgcgtca
     8401 ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg gaacagtatg ttaccaaacc
     8461 ggttgatgcc aaaattcagg accgtttcgc agaaggcatt ttgctgacgc tgatcgaaga
     8521 tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg cgcgccaacg tcatgtgggc
     8581 ggcgacgcag gcgctgaacg gtttgattgg cgctggcgta ccgcaggact gggcaacgca
     8641 tatgctgggc cacgaactga ctgcgatgca cggtctggat cacgcgcaaa cactggctat
     8701 cgtcctgcct gcactgtgga atgaaaaacg cgataccaag cgcgctaagc tgctgcaata
     8761 tgctgaacgc gtctggaaca tcactgaagg ttccgacgat gagcgtattg acgccgcgat
     8821 tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg acccacctct ccgactacgg
     8881 tctggacggc agctccatcc cggctttgct gaaaaaactg gaagagcacg gcatgaccca
     8941 actgggcgaa aatcatgaca ttacgttgga tgtcagccgc cgtatatacg aagccgcccg
     9001 ctaagctttt tacgcctcaa actttcgttt tcgggcattt cgtccagact taagttcaca
     9061 acacctcacc ggagcctgct ccggtgagtt catataaagg aggaacgtat ggctaatcca
     9121 accgttatta agctacagga tggcaatgtc atgccccagc tgggactggg cgtctggcaa
     9181 gcaagtaatg aggaagtaat caccgccatt caaaaagcgt tagaagtggg ttatcgctcg
     9241 attgataccg ccgcggccta caagaacgaa gaaggtgtcg gcaaagccct gaaaaatgcc
     9301 tcagtcaaca gagaagaact gttcatcacc actaagctgt ggaacgacga ccacaagcgc
     9361 ccccgcgaag ccctgctcga cagcctgaaa aaactccagc ttgattatat cgacctctac
     9421 ttaatgcact ggcccgttcc cgctatcgac cattatgtcg aagcatggaa aggcatgatc
     9481 gaattgcaaa aagagggatt aatcaaaagc atcggcgtgt gcaacttcca gatccatcac
     9541 ctgcaacgcc tgattgatga aactggcgtg acgcctgtga taaaccagat cgaacttcat
     9601 ccgctgatgc aacaacgcca gctacacgcc tggaacgcga cacacaaaat ccagaccgaa
     9661 tcctggagcc cattagcgca aggagggaaa ggcgttttcg atcagaaagt cattcgcgat
     9721 ctggcagata aatacggcaa aaccccggcg cagattgtta tccgctggca tctggatagc
     9781 ggcctggtgg tgatcccgaa atcggtcaca ccttcacgta ttgccgaaaa ctttgatgtc
     9841 tgggatttcc gtctcgacaa agacgaactc ggcgaaattg caaaactcga tcagggcaag
     9901 cgtctcggtc ccgatcctga ccagttcggc ggctaacatg caaattctcc cggtggcggt
     9961 aatgttccgc taccggactt ttcagaaatc atttattccc ctcgcgtccc gcccgttgtt
    10021 actcttcctt gttcaggaat gccaaatata aggacatcat catgcagagc cggaagctct
    10081 taaaagaaca actcatctat atccgggata aacgcaacgg agaggtgaaa aacagatgaa
    10141 aataatactt ctgtttttag cagccctggc aagttttacc gtacacgcgc agcctccttc
    10201 acagaccgta gaacaaacag tccggcagat ttatcagaac tataaatcag atgccagtac
    10261 gccttatttc ggtgaaaccg gagagcgggc gataacttca gcgcgtattc aacaggcgct
    10321 taccctgaac gacaatctta cgctgccggg caatattggc tggctggatt atgatccggt
    10381 ttgtgattgt caggattttg gcgatctggt gctagaaagc gttgcgataa cccaaactga
    10441 cgccgatcat gccgatgccg ttgtgcgctt tcgtatcttt aaagatgata aagaaaagac
    10501 cacgcagaca ctgaaaatgg tggcggaaaa tggtcgttgg gtcattgacg atattgtcag
    10561 caatcatggc agtgtcttac aagcagttaa tagcgagaat gaaaaaacgc tggccgcttt
    10621 agcttcgttg caaaaagaac agccggaagc ctttgttgcc gaactctttg aacatattgc
    10681 tgattatagc tggccgtgga cgtgggtggt ttccgactct taccgccagg cggttaatgc
    10741 cttctataaa accaccttca agacggccaa taatcccgat gaagatatgc aaatagaacg
    10801 gcaatttatt tacgacaatc cgatctgttt tggcgaagag tcgctatttt cacgcgttga
    10861 tgaaattcga gtcctggaga aaaccgccga ttccgcccgc attcatgttc gttttacgct
    10921 gaccaatggc aacaacgaag agcaagaact ggttttacag cggcgcgaag gcaagtggga
    10981 aatcgctgat tttatccgcc cgaacagcgg cagcctactt aagcagattg aggcaaaaac
    11041 tgccgccaga ttaaagcaat gagctgaatt aaataacaat tagccggaac aataaataaa
    11101 agggaacact atatgaaaac gattttcacc gtgggagctg ttgttctggc aacctgcttg
    11161 ctcagtggct gcgtcaatga gcaaaaggtc aatcagctgg cgagcaatgt gcaaacatta
    11221 aatgccaaaa tcgcccggct tgagcaggat atgaaagcac tacgcccaca aatctatgct
    11281 gccaaatccg aagctaacag agccaatacg cgtcttgatg cacaggacta ttttgattgc
    11341 ctgcgctgct tgcgtatgta cgcagaatga taaaaaaatc cccggcagca tgtcagttgc
    11401 cgggttatag gctgtttgct aaaaaaagcc aaacataagg acatgattat gcgacgctgg
    11461 cacacctgtt tttttattct gctgttctgt ggcttcagcg gaaacgtgtt gtgcaaagaa
    11521 tcgattaatg aagcgataga acgtttgtac caaccattga ccagtaatga cgatatacca
    11581 ataaccgatg aattcagtct cgatttgcga agtaatatga gcgctactcc tggcaaaatg
    11641 acaccctggc tgaataataa tcttttgtgt cgctgtgtcg atgacgcttt cgtttctgca
    11701 gaaataatcc aaagcacaca cctaaatggc ggccgaacgc atgtactgtt gcagctggtt
    11761 atcaaacaaa
//
LOCUS       DAJTNN010000129        11528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTNN010000129 DAJTNN010000000
VERSION     DAJTNN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.129"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..947)
                     /locus_tag="OO150_003039"
     CDS             complement(<1..947)
                     /locus_tag="OO150_003039"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5517106.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPREN"
     gene            complement(1156..1815)
                     /locus_tag="OO150_003040"
     CDS             complement(1156..1815)
                     /locus_tag="OO150_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5517107.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(2142..2498)
                     /locus_tag="OO150_003041"
     CDS             complement(2142..2498)
                     /locus_tag="OO150_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5517108.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2565..2859)
                     /gene="yebG"
                     /locus_tag="OO150_003042"
                     /pseudo
     CDS             complement(2565..2859)
                     /gene="yebG"
                     /locus_tag="OO150_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            2993..4171
                     /gene="purT"
                     /locus_tag="OO150_003043"
     CDS             2993..4171
                     /gene="purT"
                     /locus_tag="OO150_003043"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5517109.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(4227..4868)
                     /gene="kdgA"
                     /locus_tag="OO150_003044"
                     /gene_synonym="eda"
     CDS             complement(4227..4868)
                     /gene="kdgA"
                     /locus_tag="OO150_003044"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5517110.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4905..6716)
                     /gene="edd"
                     /locus_tag="OO150_003045"
     CDS             complement(4905..6716)
                     /gene="edd"
                     /locus_tag="OO150_003045"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5517111.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATSGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRRFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(6951..8426)
                     /gene="zwf"
                     /locus_tag="OO150_003046"
     CDS             complement(6951..8426)
                     /gene="zwf"
                     /locus_tag="OO150_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5517112.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8763..9632
                     /gene="hexR"
                     /locus_tag="OO150_003047"
                     /gene_synonym="yebK"
     CDS             8763..9632
                     /gene="hexR"
                     /locus_tag="OO150_003047"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5517113.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9760..11202
                     /gene="pyk"
                     /locus_tag="OO150_003048"
     CDS             9760..11202
                     /gene="pyk"
                     /locus_tag="OO150_003048"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5517114.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(11262..11480)
                     /locus_tag="OO150_003049"
     CDS             complement(11262..11480)
                     /locus_tag="OO150_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517115.1"
                     /translation="MEYQADYYITINDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGM
                     RRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         2787 a         2964 c         2886 g         2891 t
ORIGIN      
        1 atgttttcgc gtggcggaat taactcttcc cactgttgct catcacgcag gcgggtacgg
       61 tataagccaa agtttttgcc gtggcggttg gaacgcagat aaaaccgatg ctggtagtga
      121 tcaaggctgt attcgtgatc tttgcggcgc ggcagaaaaa caaacggctc ggcatcggcc
      181 atttccgcgt ccagcaggcg aacttcactg gtggtggcgc tggccaaatg aatgactaca
      241 tagtgcttcg aggtcgtttt atgcaggctg acgtaatagg tatcgtcttt ttcttcgtag
      301 atcagtttat cttgcgatgc tggcgtaccg atggcgtgac gccagacctg ataaggcagc
      361 agcgtcaccg gatgcttgcg aacatagtag aaagtccagg agtcatttgc ccagacaaag
      421 ctgggttcaa cgttatccag cagttccggg taccagttac cagtttccag attacgaaaa
      481 cgaatgccgt actggcgtcg ggaaagaaaa tcttctgcca gcgccataat ggtgttatcg
      541 ggcgtaatcg ccattccgcc catcgaataa aactcactat gagctgcgcg cttattggca
      601 tcgagcaatg tttcccactc atcccactct tcactgaatg ccgattgacg ctggtagata
      661 gcatattcac agcctggttc ataaatatgc cgatagcggt agccattttt gatgtagggc
      721 gcagaaactt ctcgttgcgg aatgcggtcg atgatttcct ttaagatgcg atcctgcaag
      781 gcttgttgtg aggccatcac ccgatgaccg taactatttt cttgttgcag gtagtccagg
      841 acttctggct gagaacgcgt atcgtcccgc agccagtagt aattatcgat gcgcgtatcg
      901 ccatgaagcg tcatggcgtg gggaatgcgg gcggcttttg gtagcatgtt attgttcttt
      961 ctggttgaaa catcttataa gggtggcaaa actcaccggg gatgcaagcg aaacagggga
     1021 gtcattgctt agatgatgac aggtaatggc gcggatatcg aatgttatgc agacagagaa
     1081 aatcagcctg ttcaaatggc tgtgcgattc tggatagccc gaaatagtca acttcaggct
     1141 atccagagag cggaattatt ccgccaaagt gcgtttttgc tgttcgagat cgcgttcaat
     1201 gccgtcacga acatcctggg ggattttcag cgcgtcaccc agtgcattca ggtaactgcg
     1261 ttccataaaa tggtcaatat caatagccgc gcaactcagg aaatagattt ccagcgcctc
     1321 ttcttcattg cggaccccgg tagccaagcg ttgtggatcc agcggttgtt cgattgcctg
     1381 ctcaatgagt acacgcccct gctcttccac gccggcttca cgcaattgct ggtcgattgc
     1441 cgcacgttct ttggcatcaa tatgaccatc acttttagcg gcaaagacca gcgcaaggat
     1501 caaacgttct gtacgctcat ccagcggcgt actttgcgcg ccaaactgcg gttcgtcctg
     1561 atgcgccgcg cgaattttat ctttgtattt attccacagc accgtacccg ctaccgctcc
     1621 gccgccaacc agtaacgcgt tggtgccata tttcgtaaga agtttacgtg ctgatttatt
     1681 tgcgaccagc agcccagcca gtccgcctaa tgcgcctggc actaacagtt tgcccaatcc
     1741 ctgatccgca gacgaggaga tagaagaact gctttgccca agcagggatt gcagttgatt
     1801 taaccagtta gccatatttg ctcctcaata accattttta gtgcctgtag catagcggat
     1861 gaggatgtca gaaaatgtat atcgagacaa aagatgcgca aataagcggg gcggtaggcc
     1921 gttattcaaa gaaattcgcg ccaggtaagg tatttactgt atatcaggtg ataagggtat
     1981 tttaggtgaa agtagtttgt cgttttgagt ggcattgttt gatgctgatt gcccgatgcg
     2041 acgctgttgc gtcttctcag gcctacgatt gtggtgcgtt ctggggcgga caaggcactc
     2101 gcgccgcatc cgccaacggt caatcgaatt atcagaagcc attaacgccg ctgatattcc
     2161 gccattcccg ctttgcagtc cacgctgacc tggtaatgaa tatcggcact tttaccacgc
     2221 acggttagcg gtactgacca tttatcatct ttaccctgaa tgtcctgcaa actgacccaa
     2281 gccacgggat cggcctgacc gacaattttt tgatcatctg cccaacgcgc cacgcgattt
     2341 tgttgataat cacgttttac gctcgcggca attccggcag catccagacc ttcacacttt
     2401 gggaaagtga ccgacttgct ggtttcatta ttggcagcga aaactgatgc gcaggcagaa
     2461 accaacaaca gccctaaaaa cgcccctctt tttttcatgt ttttctccat agcacaatga
     2521 ttcaggagaa agcatggtac aaattgtcag gagcgcaagt tgcttcaggc cgcgtgtgag
     2581 gcatcttcct cttcggattc agcaccgacg acctgtgggt ggggacggta atttgccggt
     2641 tttcagaatg gtgctcagca catctttttg ttctgccagc catagcgaaa gggcttcacg
     2701 ttgctcgtct tccatttgca ctggagaatt agtcagccag gtgtcaagca gatccgccgt
     2761 atcaagcatt ttgtcataag catcggcttc ctttttgctg gtaaacgaca ttttctcttc
     2821 gccctcacga atgactacgt atttaacttc aaccgccatt tgcagcctct cataataact
     2881 gtgattttat acagtgtatt tcttttcggt tgagaaatca aaatcaacaa taaagacaca
     2941 cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag gggatattcg ttatgacgtt
     3001 attaggcact gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct ccggtgaact
     3061 gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg ccgtcgatcg
     3121 ctatgccgac gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta atatgcttga
     3181 tggtgatgca ttacgccgtg tggttgaact ggaaaaacca cattatatcg tgccggagat
     3241 cgaagctatt gccaccgata tgctgatcca acttgaagag gaaggactga atgttgtccc
     3301 ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc tggcggcaga
     3361 agagctgcag ctgcccactt ccacttatcg ttttgccgat agcgaaagcc ttttccgcga
     3421 ggcggttgct gacattggct atccctgcat tgtaaaaccg gtgatgagct cttccggcaa
     3481 ggggcagacg tttattcgtt ctgcagagca acttgctcag gcatggaagt acgctcagca
     3541 aggcggtcgc gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt ttgacttcga
     3601 aattaccttg ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac cggtaggtca
     3661 tcgccaggaa gatggcgact accgtgaatc ctggcaacca cagcaaatga gcccgcttgc
     3721 ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg gttatggtct
     3781 gtttggtgtc gagctgtttg tctgtagtga tgaggtgatt ttcagtgagg tctcccctcg
     3841 tccacatgat accgggatgg tgacgttaat ttctcaagat ctttcagagt ttgccctgca
     3901 tgtacgtgcc ttcctcggac ttccggttgg cgggatccgt cagtatggtc ctgcagcttc
     3961 tgccgttatt ctgccacaac tgaccagtca gaatgtcacg tttgataatg tgcagaatgc
     4021 cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg gaaattgatg gcagccgtcg
     4081 tctgggtgtt gcgctggcga ccgctgagag tgttgttgac gccattgaac gcgcgaagca
     4141 cgccgccgga caggtaaaag tacagggtta aacccggcaa aaaaacgcta caaaaatgcc
     4201 cgatcgtaga tcgggcgttt tgacttttac agcttagcgc cttctacagc ttcacgcgcc
     4261 agcttagtaa tgcggtcgta atcgcccgct tccagcgcat ctgccggaac cagccaggaa
     4321 ccaccgatgc acagcacgct tttcagcgcc aggtagtcac ggtagttagc cggagaaata
     4381 ccacccgtcg ggcagaaacg gacctgggag aacggacccg cgatcgcctg cagggctttc
     4441 acgccgccgt tagcttcagc cgggaagaat ttgaactctt tcaaaccgta gtccataccc
     4501 agcatcagtt cggaaacagt gctgatcccc ggaattagag gaatagtccc ttcggtagca
     4561 gctttcagca gcggctcggt cagacccggg ctaattgcga actgtgcacc cgcttcagtg
     4621 acttctgcca gctgctgtgg attcagcacc gtaccggcac ccacaatcgc ttcaggcact
     4681 tctttggcga tagcacggat agcgtcaact gcacactcgg tacgcagagt cacttccaga
     4741 acgcgcaccc caccagcaac caacgctttt gccatcggca ctgcgtgttc cagttttttt
     4801 accacgataa ccggtacaac cgggccggtg gtcaggattg attctgcact tgttttccag
     4861 tttttcatca gagttttctc tcgcctgatt acaaatttgt cgtcttaaaa agtgatacag
     4921 gttgcgccct gttcggcacc ggacagtttt tcacgcaagg cgctgaataa ttcacgtcct
     4981 gtccccacgc gtgacgcgct caggtcagga atgtgcggtt cgcgagcagc cagttccgct
     5041 tcgtctacca gcagcgtcag ttcgcctgtc tgtccattca cacgaatgat gtccccgtcg
     5101 cgcacttttg ccagcagccc gccatcgtag gcttctggtg ttacgtggat agctgacggc
     5161 actttacctg aagcgccgga gagtcgtcca tcggtaacta acgcaatttt gaaacgccgg
     5221 tccaataata caccaagtgg cggcatgagt ttatgtaatt ctggcattcc gttcgctttt
     5281 ggcccctgat gacggacaac aacgacacaa tcgcggtcca gcaaacccgc ttcaaaggcc
     5341 ggcataacgt catgctggct ttcaaaaaca accgctggcg cttcaatcac ctggttctca
     5401 accggcacgg cagaggtttt cataaccgca cggcccaggt taccgcttaa cacttttgtc
     5461 ccaccatgat gagagaaagg ttgttcgaag gaagcgatca cattgctgtc gagtgatttt
     5521 tccgcccctt cccgccagtc cagttcacca ttattcagcc atggttcaag ggtataacga
     5581 gacagaccaa agcctgccac cgtattgaca tcttcatgca gcaggcctgc tttgagcagt
     5641 tcacgcacca gaaccggtac gccacctgcc gcctggaagt ggttaatatc ggccggaccg
     5701 ttcgggtaga gacgtgccat cagcggtaca acatcagaaa ggtcagagaa gtcatcccag
     5761 ttaatctgaa taccggccgc gcgcgccatc gccaccaggt gcatggtgtg gttagtggaa
     5821 ccactggtcg ccagcagtgc aacgataccg ttcaccacca ctttctcatc gatcatctta
     5881 ccgatcggca tccattcatt accattaccg gtcatgcgtg taacctgacg cgcagcggca
     5941 gcggtcaaag catcgcgcag cggagaatcc ggatgaacaa aagaagagcc tggcgactgc
     6001 atccccataa attccaccac catctggttg gtgttggcag taccgtagaa agtacatgtt
     6061 cccggcgcat ggtaagacgc ggcttctgac tccagtaagg ccatgcggtc cactttacct
     6121 tcggcataaa gctggcgaat acgcactttt tctttatttg gcaaaccgct tgccatcggt
     6181 ccagacggca caaacaccgc aggcaaatga ccaaacgaca gggctgccat cgtcagaccc
     6241 gggacaatct tgtcgcacac accgaggaac agagcaccat caaacatgtt atgggacagc
     6301 cccaccgccg cagacatcgc tatcacttcg cggcttagca gcgacaattc cattccatcc
     6361 tgcccctggg tgacaccatc acacatcgcc ggaacaccgc ccgcaacctg accaaccgca
     6421 ttcgcttcat gcagggcttt acgaatgatt tctggatagt gttcataagg ctggtgcgcg
     6481 gagagcatgt cgttatagga ggtgatgatg gcgatattgt tacgcaacat gcttttcaaa
     6541 gaggctttgt cttctggctg gcaggcagcg aaaccgtgtg ccaggttacc gcatgccaac
     6601 tgcgaacgat gaacggtcga cgttttcgct tgttctatcc gggcgagata agcagagcga
     6661 gtctcgcgcg aacgttcaat gattcgattt gttacgcgta acaattgtgg attcataaag
     6721 gctcctgaaa ttgagttgtc agagcaggat gattcacaac gcgtttcatt cagaggattt
     6781 atgagtgaaa cgcctgtaac cggagctcat agggcaaaaa cgtttcagtc agtgtaataa
     6841 aaaaagcctc gtgggtgaat ccgcacgagg cctgaaagtg taaaaattgt tctacaatct
     6901 gtgcaagatc atgttaccgg taaaataacc ataaaggata agcgcagata ttactcaaac
     6961 tcattccagg aacgaccatc acgggtaatc atcgccaccg aggcaacggg tccccaggtt
     7021 ccggcctgat acggtttcgg cgcatcattg tccatcgccc acgcctcagt aatggagtct
     7081 acccatttcc aggcttcttc cacttcatca cgacgtacaa acagtgcctg aataccacgc
     7141 atggtttcca gcagcagacg ttcataggca tccgccagat gcgtctgatt aaaggtttct
     7201 gaatagctca gatccagctt ggtgatttgc aggttatgtt tgtggtcaag gccaggaact
     7261 ttattcagta cctggatatc cacgccttca tcaggttgca gacggatagt cagtttattc
     7321 tgcggcagat cctgccacga ctctttaaac agattcagtt caggtgtttt gaaatagacc
     7381 acgacttcag aacatttggt cggcagacgt ttaccagtac gcaggtagaa tggcacaccg
     7441 gcccagcgcc agttatcaat gtcgacgcgg atcgccacga aggtttctgt attgctgctc
     7501 ttgttcgcac cctcttcttc cagatatccc ggcacttttt tgccctgggc gaagcccgca
     7561 gtatattgcc cacgtacggt tttttcgcgt acgttggagc ggtcgatgcg gcgcagggac
     7621 ttcagtactt tcactttttc atcgcggatg ctgtctgcgc tcaggtcaga tggcggagac
     7681 atcgcaatca tgcagagaat ttgtagcagg tggttctgaa tcatgtcacg catctgaccg
     7741 gctttatcaa aatagcccca gcgcccttcg atccccactt cttctgccac ggtaatctca
     7801 acatgatcaa tggtgcgatt gtcccagtta ttcacaaaca gggagttagc aaaacgcagc
     7861 gccaacaggt tcagcaccgt ttctttacca agatagtggt cgatacggta aacctggcac
     7921 tcctcgaagt attcgccaac ctgatcattg atttcctgcg aggtcgccag cgacgtcccc
     7981 agcggtttct ccatgactac gcgtgccggt ttagcattca gttttgcctc gccaagccct
     8041 ttgcaaattg cgccaaaagt gctgggcggc atggcaaagt agttaatggt gatacgattt
     8101 ttttgatcca gcatcgcgcc gagacggttg aatgcagcag tgtcattgac atcgagatta
     8161 caaaaatcca gacgtgcact cagggtgtcc cataaacctt catcaatggt ttctttcatg
     8221 aaagtttcga gcgcctcgcg gacaactttg gtatatgccg ctttatccca gtcagcacgc
     8281 cctacgccga taatccgggt gtccgggttg agctgaccgg ctttttccag ttgatacagg
     8341 gaaggcagca atttacgacg cgcaaggtcg cctttcgcgc cgaaaatgac caggtcacag
     8401 gcctgggctg tttgcgttac cgccatgtca ttctccttaa gttaactaac ccggtactta
     8461 agccagggta tacttgtaat tttcttacgg tgcactgtac tgcttttacg agcttgcgaa
     8521 aactgtaaac gcttatccac ccgtgcgatt acgggaaaag cgcgcaaagc gcggcaaaac
     8581 tgataaaaaa tcatcgtttt ccattcggta aaaatctgac actgatcatg ttatgaaaaa
     8641 aaataacaac ttttttatct gcttttgtca ttaacacggc acacaggcgt aatatctgac
     8701 aaaactgcat ttcgatttct ttcagtgcgg aaatcgtcat tacccgtgag tctctttaca
     8761 tcatgaatat gctggaaaaa atccagtctc agctggaaca tttgagcaaa tcagagcgca
     8821 aagttgccga ggtcattctg gcttcgcccg ataacgcgat ccattcgagt attgctgcta
     8881 tggcactgga agccaatgtt agcgaaccga cggtgaatcg tttctgtcgc agcatggaca
     8941 cgcgcggttt tcctgatttt aaacttcatc tggcacagag tctggcgaat ggcactccct
     9001 atgttaatcg caatgtcaat gaagatgaca gcgttgaatc atacacaggg aaaatatttg
     9061 agtccgcaat ggcaacgctt gatcatgtcc gtcattcact ggataaatct gccatcaacc
     9121 gcgccgtcga cttgctcact caggcaaaaa aaatcgcctt tttcggatta ggctcttcag
     9181 ccgccgttgc ccacgatgcg atgaataagt tctttcgttt taatgttccg gtggtgtact
     9241 ccgatgatat cgtgctgcaa cgcatgagtt gtatgaattg tagcgacgga gacgtggtgg
     9301 tgctgatttc tcacactgga agaacaaaaa atctggtcga gctggcgcag ctggctcgcg
     9361 aaaacgacgc aatggtgatt gccctcacct ctgcgggtac cccgctcgcc cgggaagcaa
     9421 cgctggcaat taccctcgac gtaccggaag atactgacat ttatatgccc atggtttctc
     9481 gacttgcaca gctgaccgtg atagatgtgc tggcgacagg atttactttg cgacgcggtg
     9541 caaaattcag agataacttg aagcgggtca aggaagcgct gaaggaatcg cgttttgata
     9601 agcagttact taatttaagt gacgatcgct aaaaacgact gtcactgtcc taatcttata
     9661 cgacatccga atgagattaa tttatcgcca tcgcggcgtt atttcattcg gatttcatgt
     9721 tcaagcaaca cctggttgtt tcagtcaacg gagtattaca tgtccagaag gcttcgcaga
     9781 acaaaaatcg ttaccacgtt aggcccagca acagatcgcg ataataatct tgaaaaagtt
     9841 atcgcggcgg gtgccaacgt tgtacgtatg aacttttctc acggctcgcc tgaagatcac
     9901 aaaatgcgcg cggataaagt tcgtgagatt gccgcaaaac tggggcgtca tgtggctatt
     9961 ctgggtgacc tccaggggcc caaaatccgt gtatccacct ttaaagaagg caaagttttc
    10021 ctcaatattg gggataaatt cctgctcgac gccaacctgg gtaaaggtga aggcgacaaa
    10081 gaaaaagtcg gtatcgacta caaaggcctg cctgctgacg tcgtgcctgg tgacatcctg
    10141 ctgctggacg atggtcgcgt ccagttaaaa gtactggaag ttcagggcat gaaagtgttc
    10201 accgaagtca ccgtcggtgg tcccctctcc aacaataaag gtatcaacaa acttggcggc
    10261 ggtttgtcgg ctgaagcgct gaccgaaaaa gacaaagcag acattaagac tgcggcgttg
    10321 attggcgtag attacctggc tgtctccttc ccacgctgtg gcgaagatct gaactatgcc
    10381 cgtcgcttgg cacgcgatgc aggatgtgat gcgaaaattg ttgccaaggt tgaacgtgcg
    10441 gaagccgttt gcagccagga tgcaatggat gacatcatcc tcgcctctga cgtggtaatg
    10501 gttgcacgtg gcgacctcgg tgtggaaatt ggcgacccgg aactggtcgg cattcagaaa
    10561 gcgttgatcc gtcgtgcgcg tcagctaaac cgagcggtaa tcacggcgac ccagatgatg
    10621 gagtcaatga ttactaaccc gatgccgacg cgtgcagaag tcatggacgt agcaaacgcc
    10681 gttctggatg gtactgacgc tgtgatgctg tctgcagaaa ctgccgcagg gcagtatccg
    10741 tcagaaactg ttgcagccat ggcgcgcgtt tgcctgggtg cggaaaaaat cccgagcatc
    10801 aacgtttcta aacaccgtct ggacgttcag ttcgacaatg tggaagaagc tattgccatg
    10861 tcagcaatgt acgcagctaa ccacctgaaa ggcgttacgg cgattattac catgaccgaa
    10921 tcgggtcgta ccgcgctgat gacctcccgt atcagctctg gtctaccaat tttcgccatg
    10981 tcgcgccatg aacgtacgct gaacctgact gctctctatc gtggcgttac gccggtgcac
    11041 tttgatagcg ctaatgacgg cgtagcagct gccagcgaag cggttaatct gctgcgcgat
    11101 aaaggttact tgatgtctgg tgacctggtg attgtcaccc agggcgacgt gatgagtacc
    11161 gtgggttcta ctaataccac gcgtatttta acggtagagt aatctcacct gccccggccg
    11221 ctgatggccg gggtatttag caacacataa attaggacta cttattcaat ccaatcataa
    11281 tcacagcccc ctgtaaaatg aatacaggga tagcctgaat ggagatctaa atagcttgca
    11341 agacgtcgca tacccgcttc ctctgcaaca ctatgtccaa taactgccat cggtaagcca
    11401 ctatctttgg agtattgtgt ctgcacccat gttttaattg aatcgttaat cgtaatataa
    11461 taatcagcct gatattccat atattgtatt ggatcacaaa aacagcctgc cccaatgcca
    11521 acagttct
//
LOCUS       DAJTNN010000130        11379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTNN010000130 DAJTNN010000000
VERSION     DAJTNN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.130"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            216..1325
                     /gene="livK"
                     /locus_tag="OO150_003050"
     CDS             216..1325
                     /gene="livK"
                     /locus_tag="OO150_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5517116.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1373..2299
                     /gene="livH"
                     /locus_tag="OO150_003051"
     CDS             1373..2299
                     /gene="livH"
                     /locus_tag="OO150_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5517117.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2296..3573
                     /gene="livM"
                     /locus_tag="OO150_003052"
     CDS             2296..3573
                     /gene="livM"
                     /locus_tag="OO150_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5517118.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3570..4337
                     /gene="livG"
                     /locus_tag="OO150_003053"
     CDS             3570..4337
                     /gene="livG"
                     /locus_tag="OO150_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5517119.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4339..5052
                     /gene="livF"
                     /locus_tag="OO150_003054"
     CDS             4339..5052
                     /gene="livF"
                     /locus_tag="OO150_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5517120.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRAASGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5449..6120
                     /locus_tag="OO150_003055"
     CDS             5449..6120
                     /locus_tag="OO150_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR5517121.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENRHLPGDRRRHVRRAGSGH
                     HDADL"
     gene            6050..6253
                     /locus_tag="OO150_003056"
                     /pseudo
     CDS             6050..6253
                     /locus_tag="OO150_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
     gene            6250..7095
                     /gene="ugpE"
                     /locus_tag="OO150_003057"
     CDS             6250..7095
                     /gene="ugpE"
                     /locus_tag="OO150_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5517122.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATTTF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            7097..8167
                     /gene="ugpC"
                     /locus_tag="OO150_003058"
     CDS             7097..8167
                     /gene="ugpC"
                     /locus_tag="OO150_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR5517123.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIVLSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            8164..8907
                     /gene="ugpQ"
                     /locus_tag="OO150_003059"
     CDS             8164..8907
                     /gene="ugpQ"
                     /locus_tag="OO150_003059"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5517124.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVVGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(8894..9334)
                     /locus_tag="OO150_003060"
     CDS             complement(8894..9334)
                     /locus_tag="OO150_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5517125.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            9454..11187
                     /gene="ggt"
                     /locus_tag="OO150_003061"
     CDS             9454..11187
                     /gene="ggt"
                     /locus_tag="OO150_003061"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5517126.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAVAAPPVSYGVEEDIFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAQPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(11225..>11379)
                     /locus_tag="OO150_003062"
     CDS             complement(11225..>11379)
                     /locus_tag="OO150_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517127.1"
                     /translation="ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKK
                     ATFGLP"
BASE COUNT         2533 a         2866 c         3286 g         2694 t
ORIGIN      
        1 aaaggcactt ttttctgttt atctatcaat aaattcagaa tattatctgt tcttaatcga
       61 ctgaaagata gggattttaa tcactattat cacaaaatac tgcgctaaac ccttaatcag
      121 acaggcaaaa acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca
      181 aagcaacaca acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca
      241 tcgcagggat gattgcactg gcaatttcac acaccgctat ggctgacgat attaaagtcg
      301 ccgttgtcgg cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg
      361 cgcgtcaggc gattaaagac attaatgcca aagggggaat taagggcgac aagctggttg
      421 gcgtggaata tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg
      481 ttaatgacgg cattaaatac gttattggtc atctgtgttc ttcttctacc caacctgcat
      541 cagatatcta cgaagacgaa ggtattctga tgatctcacc gggagcgacc aacccggagc
      601 tgacccaacg cggttatcaa cacattatgc gtactgccgg gctggactct tcccaggggc
      661 caacggcggc aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg
      721 acaaacaaca gtatggcgaa gggctggcgc gttcggtgca ggacgggctg aaagcggcta
      781 acgccaacgt cgtcttcttc gacggtatta ccgccgggga gaaagatttc tccgcgctga
      841 tcgcccgcct gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa
      901 tggggcagat gctgcgccag gcccgttccg ttggcctgaa aactcagttt atggggccgg
      961 aaggtgtggg taacgcatca ttgtcgaata ttgccggtga tgctgccgaa ggcatgttgg
     1021 tcactatgcc aaaacgctat gaccaggatc cggcaaacca gggcatcgtt gatgcgctga
     1081 aagcagacaa gaaagatccg tccgggcctt atgtctggat cacctacgcg gcggtgcaat
     1141 ctctggcgac tgcccttgag cgtactggca gcgatgagcc gctggcgctg gtgaaagatt
     1201 taaaagctaa cggtgcaaac accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc
     1261 ttaagggatt tgattttggt gtcttccagt ggcacgccga cggttcatcc acggcagcca
     1321 agtgatcatc ccaccgcccg taaaatgcgg gcgggtttag aaaggttacc ttatgtctga
     1381 gcagtttttg tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacctacgc
     1441 gctgatagcc atcggctaca ccatggttta cggcattatc ggcatgatca acttcgccca
     1501 cggcgaggtt tatatgattg gcagctacgt ctcatttatg atcatcgccg cgctgatgat
     1561 gatgggcatt gataccggct ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat
     1621 tgccagcgcc tacggctgga gtatcgaacg ggtagcctat cgcccggtgc gtaactctaa
     1681 gcgcctgatt gcgctcatct cggcaatcgg catgtccatc ttcctgcaaa actacgttag
     1741 cctgactgaa ggttcgcgtg acgtggcgct gcccagtctg tttaacggtc agtgggtggt
     1801 ggggcatagc gaaaacttct ctgcctctat taccaccatg caggcagtga tctggattgt
     1861 tactttcctc gccatgctgg cgctgacgat tttcattcgt tattcccgta tggggcgcgc
     1921 ctgccgtgcc tgcgcggaag atctgaaaat ggcgagcctg cttggcatca acaccgaccg
     1981 ggtgattgct ttgacctttg ttattggcgc ggcgatggcg gcagtggctg gcgtactgct
     2041 cggtcagttc tacggcgtca ttaaccccta tatcggcttt atggccggga tgaaagcctt
     2101 taccgctgcg gtgctcggtg ggattggcag cattccaggg gcgatgattg gtgggctgat
     2161 cctggggatt gcggaggcgc tctcttctgc ctatctgagt acggaatata aggatgtggt
     2221 ttcatttgcc ctgctgattc tggtgctgct ggtgatgccg accggtattc tgggtcgccc
     2281 ggaggtagag aaagtatgaa accgatgcat attgcaatgg cgctgctctc tgccgcgatg
     2341 ttttttgtcc tggcgggcgt ctttatgggc gtgcaactgg agctggatgg caccaaactg
     2401 gtggtcgaca cggcttcaga catccgttgg cagtgggtgt ttatcggcac ggcggtggtc
     2461 tttttcttcc agcttttgcg accggctttc cagaaagggc taaaaagcgt ttccggaccg
     2521 aagtttattc tgcccgcgat tgatggctcc acggtgaagc agaaactgtt cctcgtggcg
     2581 ctgttggtgc ttgcggtggc gtggccgttt atggtgtcac gtgggacggt ggatatcgcc
     2641 actctgacca tgatctacat tatcctcggt ctcgggctga atgtggttgt tggtctttct
     2701 ggtctgctgg tgctggggta cggcggtttt tacgccatcg gcgcttacac ctttgcgctg
     2761 ctcaatcact attacggctt gggcttctgg acttgtctgc cgattgccgg gttaatggca
     2821 gcggcggcgg gcttcctgct cggctttccg gtgctgcgtc tgcgcggtga ttatctggcg
     2881 atcgttaccc tcggcttcgg cgaaattgtg cgcatattgc tgctcaataa caccgaaatt
     2941 accggcggcc cgaacggaat cagtcagatc ccgaaaccga ctttcttcgg ccttgagttc
     3001 agccgtaccg cccgtgaagg cggttgggac acgttcagta atttctttgg cctgaaatac
     3061 gatccctccg atcgcgtcat cttcctgtat ctggtggcgt tgctgctggt ggtgctaagc
     3121 ctgtttgtca ttaaccgcct gctgcggatg ccgctggggc gtgcgtggga agcgttgcgt
     3181 gaagatgaga tcgcctgccg ttcgctgggc ttaagcccgc gtcgtatcaa gctgaccgct
     3241 tttaccatca gtgccgcgtt tgccggtttt gccggaacgc tgtttgctgc gcgccagggc
     3301 tttgtcagcc cggaatcctt cacctttgct gaatcggcgt ttgtactggc gatagtggtg
     3361 ctcggcggta tgggatcgca gtttgcggtg attctggcgg caattttgct ggtggtgtcg
     3421 cgcgagttga tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg
     3481 ctgatgatga tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actgaagctg
     3541 aaaaacggcg cagcgaaagg agagcaggca tgagtcagcc attattatct gtgaacggcc
     3601 tgatgatgcg cttcggcggc ctgctggcgg tgaacaacgt caatcttgaa ctgtacccgc
     3661 aggagatcgt ctcgttaatc ggtcctaacg gtgccggaaa aaccacggtc tttaactgcc
     3721 tgaccggatt ctacaaaccc actggaggca ccattttact gcgcgatcag catctggaag
     3781 gtttaccggg gcagcaaatt gcccgcatgg gcgtggtgcg taccttccag catgtgcgtc
     3841 tgttccgtga aatgacggtg attgaaaacc tgctggtggc gcagcatcag caactgaaaa
     3901 ccgggctgtt ctctggcctg ttgaaaacgc catccttccg tcgcgcccag agcgaagcgc
     3961 tcgatcgcgc cgcgacctgg cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg
     4021 cgagtaacct ggcctatggt gaccagcgcc gtcttgagat tgcccgctgc atggtgacgc
     4081 agccggagat tttaatgctc gacgaacctg cggcaggtct taacccgaaa gagacgaaag
     4141 agctggatga gctgattgcc gaactgcgca atcatcacaa caccactatc ttgttgattg
     4201 aacacgatat gaagctggtg atgggaattt cggaccgaat ttacgtggtc aatcagggga
     4261 cgccactggc aaacggtacg ccggagcaaa tccgtaataa cccggacgtg atccgtgcct
     4321 atttaggtga ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac
     4381 tacggcaaaa tccaggcact gcatgaggtg agcctgcata tcaatcaggg cgagattgtc
     4441 acgctgattg gcgcgaacgg ggcggggaaa accaccttgc tcggcacgtt atgcggcgat
     4501 ccgcgcgccg ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg
     4561 gcgaaaatca tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg
     4621 atgacggtgg aagagaacct ggcgatgggc ggcttttttg ccgaacgcga ccagttccag
     4681 gagcgcataa agtgggtgta tgaactgttt ccacgtctgc atgagcgccg tgttcagcgg
     4741 gcaggcacca tgtccggcgg tgagcaacaa atgctggcga ttggtcgcgc gctgatgagc
     4801 aacccgcgtt tgctgctact tgatgagcca tcgctcggtc ttgcgccgat tatcatccag
     4861 caaattttcg acaccatcga gcagctgcgc gagcagggga tgactatctt cctcgtcgag
     4921 cagaacgcca accaggcgct aaagctggcg gatcgcggct acgtgctgga aaacggccat
     4981 gtggtgctct ccgataccgg cgatgcgctg ctggcgaacg aagcagtgag aagtgcgtat
     5041 ttaggcgggt aataacacgt tgattgataa ggagtcaaaa gactcctttg agacaggtga
     5101 caaatgtaaa attgcctgat gcgctgcgct tatcaggcct actgggtgag ttgcaatata
     5161 ttgaatttgc acgatcttgt aggccggata aggcgtttac gccgcatccg gcatgaaacg
     5221 atgagtaatc tgtagagttt gattcagacc ttctattttt ccgcttatcc gtgccccatc
     5281 tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac cttactatct
     5341 tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt accccgcgag
     5401 cataaaacgc gtgaattcgc gcattcggta caacaagaga gataaacgat gaaaccgtta
     5461 cattatacag cttcagcact ggcgctcgga ctggcgttaa tggggaatgc acaggcagtg
     5521 acgaccattc cgttctggca ttctatggaa ggggaactgg gtaaagaggt ggattctctg
     5581 gcccaacgtt ttaacgccga aaatccggat tacaaaattg taccgaccta taaaggcaac
     5641 tacgaacaga atttaagcgc ggggattgcc gcatttcgta ccggcaacgc tccggctatt
     5701 ttgcaggttt atgaagttgg caccgccacc atgatggcat cgaaagccat taaaccggta
     5761 tatgacgtgt ttaaagaggc ggggattcaa ttcgatgagt cgcagtttgt gccgacggtt
     5821 tccggttact actccgacag caaaacgggc cacttactct cccagccatt caacagctcg
     5881 actcccgttc tctattacaa caaagacgcc ttcaagaaag cggggttaga cccggaacag
     5941 ccgccgaaaa cctggcagga tctggcggac tatgccgcga aactgaaagc ctccggcatg
     6001 aagtgcggct acgccagcgg ctggcagggc tggatccaac tggaaaatcg acaccttccc
     6061 ggtgatcgac gccgccacgt ccggcgggcc ggttcaggcc accacgacgc tgatttataa
     6121 gatctaccgc gaaggtttta ccggactgga tctggcttcg tctgccgcac agtcggtggt
     6181 gttgatgttc ctcgtcatcg tgctgacggt ggtgcagttc cgctatgttg aaagcaaggt
     6241 gcgttaccaa tgattgagaa ccgtccgtgg ctgacgatat tcagccatac catgctgatc
     6301 ctcgggatcg cggtgattct cttcccgctg tacgtggcgt ttgtcgcggc gacgctggat
     6361 aaacaggccg tctatgccgc gccgatgacg ctcatccccg gcacacatct gctggaaaat
     6421 atccacaaca tctgggttaa cggggtaggt acgaatagcg cgccgttctg gcggatgttg
     6481 cttaacagct ttgtgatggc gttcagcatt acgctcggca aaattaccgt ctcgatgctc
     6541 tcggcatttg ccattgtctg gtttcgtttt ccgctacgta acctcttctt ctggatgatt
     6601 tttatcaccc tgatgctgcc ggttgaagta cgtatcttcc cgacggtgga agtcatcgcc
     6661 aacctgaaga tgctcgacag ctacgccggt ttaacgctgc cgctgatggc ctcggcgacc
     6721 accacgtttc tgtttcgcca gttctttatg acgctgccgg atgaactggt ggaagcggcg
     6781 cggatcgacg gcgcatcgcc aatgcgcttc ttttgcgaca tcgtttttcc gctctccaaa
     6841 actaatctgg cggcgctgtt tgtgatcacc tttatttacg gctggaacca gtatttgtgg
     6901 ccgttgttga ttattaccga cgtggatctc ggcaccaccg tggcagggat caaagggatg
     6961 atcgccactg gcgaaggcac cacggaatgg aactcagtga tggcggcgat gttgttaacc
     7021 ctgatcccac cagtagtgat tgttttagtg atgcagcgtg ccttcgtgcg cggcctggtc
     7081 gatagtgaga aataagatgg caggactgaa attacaggca gtaaccaaaa gctgggatgg
     7141 caaaacccag gtcattaaac cgctgaccct tgatgtggct gatggcgaat ttatcgtgat
     7201 ggtcgggccg tctggctgcg ggaaatcgac gctgctgcgt atggttgccg ggctggagcg
     7261 ggtgaccgaa ggcgatatct ggatcaacga ccagcgcgtg actgaaatgg agccgaaaga
     7321 tcgcgggatt gcgatggtgt tccagaacta cgcgctttat ccgcatatga gcgtcgaaga
     7381 aaacatggcg tgggggctta aaattcgcgg catgggcaag cagcaaattg ccgagcgcgt
     7441 taaagaagcg gcgcgcattc tggagctgga cggtctgctc aaacgtcgcc cgcgtgagct
     7501 atccggcggt cagcgtcagc gtgtagcgat gggccgcgcg attgtgcgcg atccggcggt
     7561 gttcctgttt gatgagccgc tctctaacct cgatgccaag ctgcgcgtgc agatgcgtct
     7621 tgaactgcaa cagttgcacc gtcgcctgaa aacgacttca ctctacgtta ctcacgatca
     7681 ggttgaagcg atgacgctcg cccagcgagt aatggtgatg aacggcggcg ttgccgaaca
     7741 gattggcaca ccagttgaag tctacgaaaa gcccgccagc ctgtttgtag cgagttttat
     7801 cggcagtccg gcgatgaacc tgctgacagg ccgcgtgaat aacgaaggca cgcatttcga
     7861 actggacggc ggtattgagc tgccgctaaa cggtggctac cgtcagtatg ccgggcgtaa
     7921 aatgactctc ggcattcgcc cggaacatat tgtgctaagc tcgcaggcag aaggcggcgt
     7981 acctctggtg atggacacgc tggagatcct cggcgcagat aacctggcgc acggacgctg
     8041 gggcgagcag aagctggtgg tgcgactggc gcatcaggag cgcccgacgg caggcagcac
     8101 gctgtggctg catctggcgg aaaatcagct gcatcttttt gatggtgaaa caggacaacg
     8161 agtatgagta actggcctta tccccgcatc gtcgctcatc gtggcggcgg taagctggcc
     8221 ccggaaaaca ccctggcggc aatcgacgtc ggggcaaaat acggtcataa gatgatcgaa
     8281 tttgacgcga agttatcgaa agatggcgag atcttcctgc tccatgacga caatctcgaa
     8341 cgcaccagca acggctgggg cgtcgtgggt gaactgaact ggcaggattt actgcgcgtg
     8401 gatgcgggca gttggtacag caaagcgttt aaaggtgagc cgctgccgtt gctttcgcag
     8461 gtggcggaac gctgtcgcga acatgggatg atggcgaata tcgaaatcaa acccaccact
     8521 ggcactggac cattaacggg caaaatggtg gcgctggcgg cacgccaact gtgggccggt
     8581 atgacgccgc cgctgctgtc atcgtttgag attgatgctt tagaagctgc acagctggcg
     8641 gcaccggaac tgccgcgtgg tttattgctt gatgagtggc gcgacgactg gcgcgaactg
     8701 accgcgcggc tgggctgcgt ctctattcat ctcaatcata agttactcga taaagcgcga
     8761 gtgatgcagt tgaaagacgc cggattacgg attctggttt ataccgtcaa taaaccccag
     8821 cgcgcggcag agttgctgcg ctggggcgtg gattgcatct gtaccgatgc gattgacgtg
     8881 attggtccga actttacggc ccaatagttt tcaacggaat gtcaggctgc ggcgtgctgg
     8941 gctgattcag catgtcgccg tttctcctct ccggcagcat atgctgctga ttcaacgaac
     9001 tatccggatt acggttgctg tttaacatcc cgccgttggt gttgggcagc atttgctgcc
     9061 gggcgggatt tcgctccccc ggctgtgact gcaacacccg ctgagaatta ttgtttatct
     9121 ggttttctaa atgctgctgt tgcaactgag tttgcgtttt cagttgctga ttcagcatcc
     9181 ctttttgctg gatttgctga gtttgcatct gggtttgcat ccgctgctgg ctgggtatct
     9241 gataccccgg ctggttaggg ttgttcagag tattaatggg ctgtgcaaag ccgacaaacg
     9301 gcaggagtgc cgtaagaatc agaagtcgtt tcatcgcgta tcctcctctg aagatatcct
     9361 ttaagtttac tcgcttcccg acaaaacgat gattaattca gagttatata ccaggcttag
     9421 ctggggttgc cccttaatct ctggagaata acgatgataa aaccgacgtt tttacgccgg
     9481 gtagccattg ctgctctgct ctcaggaagt tgttttagcg ccgtcgccgc gcctcctgtt
     9541 tcttacggcg tggaggaaga tatcttccat ccggtacgcg cgaaacaggg gatggtggcg
     9601 tctgtcgatg ccactgccac acaagtgggg gtagatattc taaaggaggg cgggaatgcc
     9661 gttgatgccg ccgtggcggt gggctacgcg ctggcggtaa cgcatccgca ggcagggaat
     9721 ctgggcggtg gtggttttat gctgatccgc tcgaaaaatg gcaataccac cgctatcgat
     9781 ttccgcgaga tggcacccgc taaagcgacc cgcgatatgt tccttgatga ccagggcaac
     9841 ccggacagca aaaaatcact cacttcgcat ctggcttccg gcacgcccgg tacggtcgct
     9901 ggtttctcgc tggcgttgga taagtacggc actatgccgt tgaacaaagt ggtgcagccc
     9961 gcgttcaaac tggcacgcga tggttttatc gttaacgatg ctttggcaga cgatctcaaa
    10021 acctacggta gcgaagtgtt gccgaatcac gaaaacagca aagctatctt ctggaaagag
    10081 ggcgaaccgc tgaaaaaggg cgacaagctg gtgcaggcga acctggcaaa gagcctggag
    10141 atgattgccg aaaacggccc ggacgaattc tataaaggca cgatagcgga acagattgcc
    10201 caggagatgc agaaaaacgg tggcttgatc actaaagaag atttagcggc gtataaagcg
    10261 gtcgaacgca caccgataag tggcgattat cgcgggtatc aggtttactc catgccaccg
    10321 ccatcctccg gcgggatcca tatcgtacaa atcctcaata ttctggaaaa cttcgatatg
    10381 aagaaatacg gctttggcag cgccgatgcg atgcaaatca tggcagaagc ggagaaatac
    10441 gcctacgccg accgctcgga atatcttggc gacccggatt ttgtcaaagt accgtggcag
    10501 gcgctgacca ataaagccta tgccaaatct attgccgatc aaattgatat caataaagcg
    10561 cagccatcca gcgaaattcg ccccggcaag cttgcgcctt atgagagtaa tcaaactacc
    10621 cattactcag tggtggataa agatggtaac gcggtggcgg tgacctatac gctgaacacc
    10681 accttcggta cgggcattgt cgcgggcgag agcggtattc tgcttaataa tcagatggat
    10741 gatttctccg ctaaaccggg cgtaccgaac gtttacgggc tggtgggcgg tgatgccaac
    10801 gccgtcgggc cgaataaacg tccgctgtcg tcaatgtcac caacgattgt ggtgaaagac
    10861 ggtaaaacct ggctggtcac cggtagccct ggcgggagcc ggatcatcac tacagtgctg
    10921 caaatggtgg tgaatagcat cgattatggc atgaacgtcg ccgaagcgac caatgcgccg
    10981 cgtttccacc atcagtggtt gccggacgag ctgcgtgtcg aaaaagggtt tagcccggat
    11041 acgctcaagc tgctggaagc aaaaggtcag aaagtggcgc tgaaagaggc gatgggcagt
    11101 acacaaagca ttatggttgg gccggacggt gagttgtacg gcgcatccga cccgcgctcg
    11161 gtggatgatt taacggcggg gtactaaggt tagcggccct cttcgtggga agagggctat
    11221 tttgtcaggg caagccgaag gtagcctttt ttatttcgta atcctgtaga tattcttcca
    11281 gcggctttgc cgttcccaga gaatgaattt caccactgtc tttatcaata ataaaaggtg
    11341 cgttaccagc taagcgcgcg gcctcatctc cagtttcga
//
LOCUS       DAJTNN010000131        11309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTNN010000131 DAJTNN010000000
VERSION     DAJTNN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.131"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..429
                     /locus_tag="OO150_003063"
                     /pseudo
     CDS             1..429
                     /locus_tag="OO150_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            600..947
                     /locus_tag="OO150_003064"
     CDS             600..947
                     /locus_tag="OO150_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5517128.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            937..1299
                     /locus_tag="OO150_003065"
     CDS             937..1299
                     /locus_tag="OO150_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5517129.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            1296..1793
                     /locus_tag="OO150_003066"
     CDS             1296..1793
                     /locus_tag="OO150_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5517130.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(1801..2985)
                     /gene="emrD"
                     /locus_tag="OO150_003067"
     CDS             complement(1801..2985)
                     /gene="emrD"
                     /locus_tag="OO150_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5517131.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(3404..3493)
                     /gene="tisB"
                     /locus_tag="OO150_003068"
     CDS             complement(3404..3493)
                     /gene="tisB"
                     /locus_tag="OO150_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5517132.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            4058..4156
                     /gene="ivbL"
                     /locus_tag="OO150_003069"
     CDS             4058..4156
                     /gene="ivbL"
                     /locus_tag="OO150_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5517133.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            4262..5950
                     /gene="ilvB"
                     /locus_tag="OO150_003070"
     CDS             4262..5950
                     /gene="ilvB"
                     /locus_tag="OO150_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5517134.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQDIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            5954..6244
                     /gene="ilvN"
                     /locus_tag="OO150_003071"
     CDS             5954..6244
                     /gene="ilvN"
                     /locus_tag="OO150_003071"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5517135.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            6318..6908
                     /gene="uhpA"
                     /locus_tag="OO150_003072"
     CDS             6318..6908
                     /gene="uhpA"
                     /locus_tag="OO150_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5517136.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            6908..8410
                     /gene="uhpB"
                     /locus_tag="OO150_003073"
     CDS             6908..8410
                     /gene="uhpB"
                     /locus_tag="OO150_003073"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5517137.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            8420..9739
                     /gene="uhpC"
                     /locus_tag="OO150_003074"
     CDS             8420..9739
                     /gene="uhpC"
                     /locus_tag="OO150_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5517138.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            9877..11268
                     /gene="uhpT"
                     /locus_tag="OO150_003075"
     CDS             9877..11268
                     /gene="uhpT"
                     /locus_tag="OO150_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5517139.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
BASE COUNT         2495 a         2938 c         3197 g         2679 t
ORIGIN      
        1 ccgtcagcaa aaaccacgca atgatagtca ttgaggattt gaaggtcagc aacatgtcaa
       61 agtcagcagc gggtacggtc agcctgccgg ggcgcaatgt ccgggcaaaa tcaggtttaa
      121 accgttcgat actggatcag ggctggtatg aaatgcgccg ccagcttgag tacaagcagc
      181 tctggagtgg cggtcaggtg cttgctgttc cgccagcgtt gcgcgtgctg tggtcataca
      241 gcgaaagaaa atcgcctgtc acaaagtaaa ttcagatgcc aggtatgtgg atatacagcg
      301 aacgccgatg taaatggcgc tcgtaacatt ttagcggcgg ggcacgccgt tcttgcctgt
      361 ggagagatgg tgcagtcagg ccgcccgttg aagcaggaac ccaccgaaat gattcaggcg
      421 acagcctgaa cgtagcaggg atcctcgtcc ttcagggcgt ggaggatgtc aatttgcgct
      481 atgataccca tctgacctcc ttgtagcgga tggcaaaagc atatcaggaa acgggcggac
      541 gaaattctta aaaaaaaccg ctaaagatag agcgttagtg aaattgcaaa ggagcaacaa
      601 tgaaaatttc ccgccttgga gaagcgcccg attaccgctt ctcgctggct aatgagcgta
      661 cttttctggc gtggatccgt accgcactag gatttctggc ggcgggggtt gggcttgatc
      721 aacttgcgcc agatttcgcc acgcccgtca ttcgtgaact gctggcgttg ttgttgtgtc
      781 tgttttccgg cgggttagca atgtatggct acctgcgctg gttacgcaat gaaaaagcga
      841 tgcgtctgaa agaggacctg ccctacacca acagcttatt aatcattagc ttaatattga
      901 tggtcgtggc ggtgatcgtt atgggactgg tgttgtatgc cggatagccg caaagccaga
      961 cgcattgccg acccaggact gcagccggag cgcacatcac tggcgtggtt tcgtaccatg
     1021 ctgggctacg gcgcgttgat ggcgttggct atcaaacaca actggcacca ggcgggcatg
     1081 ttattctgga tttcgattgg cattctcgcc atcgtggcgc tgatcctctg gcactacacc
     1141 cgtaatcgca atttaatgga tgtcacgaat agcgattttt cccaatttca cgtagtccgt
     1201 gacaaatttt tgatctccct cgcggtgtta tctctcgcaa tactgtttgc tgtaacgcat
     1261 atacatcaac ttatcgtatt tattgagaga gtcgcatgac aggaagtcag gtcattgatg
     1321 ctgaagagga caggcataaa ctggttgtag agtacaagga tgctctgcaa cctgccgatt
     1381 tttatcacaa tttcaaacag cggggcatcc gctccgtaca gctcattccc tatcttgaat
     1441 ttgatgaccg gggcgatctg acggccgctt cggtgacagc agagctgtgg gggaaatttt
     1501 taattgccct gttcgagtgt tgggtgcggg cagatatcag tcgtatctcg attgagcttt
     1561 ttgacgccac cttacaaaaa tggtgtggcg gcgaaaaccc gcaaccgcgc cgtgactgcc
     1621 aggcgtgcga ctggcatcgc ctgtgcccgc acgctcggca ggagacgcca gacagcgtgc
     1681 tttgtgcggg ctatcaggcg ttttattctt actccgcacc gcatatgcgg gtgatgcgtg
     1741 atctgataaa gcagcatcgt tctcccatgg agctgatgac gatgctgcgg tgacgtgcgc
     1801 ttaaacgggc tgcccctgat gcgacatccg cgtcgccagc ggcagccagc acagcacgat
     1861 caacaatccc attaaggtca tcaataaccc caggctaccc tgaccggttt gcggcaacat
     1921 cgcagagagc gacgccagca cgccggaacc aatgttttgc agaccgccga ccagcgcgcc
     1981 agccgtgccc gccaggaagg ggaacggctc catcgcgccg ctggtcgcca gcggaaacag
     2041 catcccggca ccgaaaaaga acagcgcggc gggaacgagc agcgtccaga cattcatcac
     2101 gccaaaccag tcggggatcc acatcagcaa gccagccagc aggcagcaga taaccgactg
     2161 ccacattaac gtggagaagc gtttattggg acgtccggca aaccatgcgc caaaaaacgc
     2221 tgccggaatc ggcagaataa acaaaatact gaccgtcata ctgctcagcc ctaacaccgc
     2281 gcccatcagc acgccggagc aggcttcaaa ggcggcaatc ccggccagac cgccaatcag
     2341 catcagcaaa taacagttaa aaccgctgtt accgaaaagc gttttataac tggtaagcag
     2401 gcgcgtgcgc ggtgcatcga ccggacgcgt ttccggcatc cagcgggcca tactgaaggt
     2461 cacaccaaca caaagaacca acaaaaagag ataacaggcg cgccagttcc acatcgtatc
     2521 cagcagaccg ccgattagcg gtgcgagcaa cggactgacg agaatcccca tgtttaacag
     2581 gctgttagca tggcgcaact gtgtccgttc atataaatct cgcggcaaag tacgcgccat
     2641 tacgccgcca acgccggtgc ccatcccctg catcgcgctg gcggcaatca ataccgtcaa
     2701 actggaggtc gtgaccgcga ccagcgttgc cagcataaaa atggacattc cgacgaggat
     2761 caccggtcgg cgacccacac ggtcggaaat cgggccataa aacagctgtg agacaccgta
     2821 agtcagcaga taagcgccca ttacgctctg caccgcccct tcacggacgt tgagatcgcg
     2881 cgccatatcg gcaatagctg gaatataaat ggtttgcgcc atctgaccga cggccacgag
     2941 taataccaac atcaataaca aattgacgtt tctttgcctt ttcattatca cggatgcttt
     3001 tataaaaata attagaataa gtcactacgc attcccataa atgcgggaga ggaatctatc
     3061 acattgatga atgttagcca gatatacgcc aggaatgggg aattgtttag cggcagaata
     3121 tgtaaacaaa agcggcaata aatgttgtcg ggagggatta cgtgcgaact gtaaccctct
     3181 ccccaaaggg gcgaggggga aaccaccgtt ccctttcaga aaatgagcta aacattgcac
     3241 gggacggtcc cctcgcccct ttggggagag ggctagggtg aggggcacag gcagcgcagg
     3301 tgcaaacatt ctgctggtag aagaagcccc caactccaac ccgcacgcta aatataaaag
     3361 gggagcggtt acccgctccc ctttggtgcg acttgaatct gaattacttc aggtatttca
     3421 gaacagcatc aagcagttgc agtgctgcaa caatgagttt gaggataaga atggcgatat
     3481 ccaccaggct catacgcgtc tcctgtggtt caggagcact gccagctgac gtacctttcc
     3541 gctgcctgtt gccagctgtt gtgcacgcgg tatctcaagg agaaacgttg tgcttgttga
     3601 cataacacag tgtgctcacg cggggttaag gcgctgacgg caccacccgt ttcagccagg
     3661 acttagtgcg ccgggtaacg atgcggcccc cgcataatac attgcgtaca gtgatattat
     3721 agaagtttac tgtataaata aacagtgata tttggacaaa acgcaaactg tgatcgatac
     3781 tcaactatgc cctaaatacg tcaaaattcg tgccgaaatt gcgcgttctg cgcggaacac
     3841 gtatactttc agtgttgaca taatacagtg tgctttgcgg ttaccagctg caggcggctg
     3901 atgaaacctc gctccggcgg ggttttttgt tacctgcaat tcagtacaaa acgtgatcag
     3961 cccctcaatt ttcccttgct gaaaaatttt ccattgtctc ccctgtaaag ctgtgcttgt
     4021 ataaatattg ttaaacacaa aaccaacaag gtccccaatg actacttcca tgttcaacgc
     4081 aaaactacta ccaactgcgc tatccgccgc agtggtcgtc gtgcgtgtgg tggtggtcgt
     4141 cggcaatgcg ccgtagggac tggaacaaca cacgattcca aaaccccgcc ggcgcaaacc
     4201 gggcggggtt tttcgtttaa gcacctcccg gaaagtcggc ccagaagaaa aggactggag
     4261 catggcaagt tcgggcacaa catcgacgcg taagcgcttt accggcgcag aatttatcgt
     4321 tcatttcctg gaacagcagg gcattaagat tgtgacgggc attccgggcg gttctatcct
     4381 gcctgtttac gatgccttaa gccaaagcac gcaaatccgc catattctgg cccgtcatga
     4441 acagggcgcg ggatttatcg ctcaggggat ggcgcgcacc gacggtaaac cggcggtctg
     4501 tatggcctgt agcggaccgg gtgcgactaa cctggtgacc gccattgccg atgcgcggct
     4561 ggactccatc ccgctgattt gcatcaccgg tcaggttccc gcctcgatga tcggcaccga
     4621 cgccttccag gaagtggaca cctacggcat ctctatcccc atcaccaaac acaactatct
     4681 ggtcagacat atcgaagaac tcccgcaggt catgagcgat gccttccgca ttgcgcaatc
     4741 aggccgccca ggcccggtgt ggatagacat tcctaaggat gtgcaaacgg cagtttttga
     4801 gattgaaaca cagcccgcta tggcagaaaa agccgccgcc cccgccttta gtgaagaaag
     4861 cattcgtgac gcagcggcga tgattaacgc tgccaaacgc ccggtgcttt atctgggcgg
     4921 cggtgtgatc aatgcgcccg cacgggtgcg tgaactggcg gagaaagcgc aactgcctac
     4981 caccatgact ttaatggcgc tgggcatgtt gccaaaagcg catccgttgt cgctgggtat
     5041 gctggggatg cacggcgtgc gcagcaccaa ctatattttg caggaggcgg atttgttgat
     5101 agtgctcggt gcgcgttttg atgaccgggc aattggcaaa accgagcagt tctgtccgaa
     5161 tgccaaaatt attcatgtcg atatcgatcg cgcagagctg ggtaaaatca agcagccgca
     5221 tgtggcgatt caggcggatg ttgatgacgt gctggcgcaa ttgatcccgc aggtggaagc
     5281 gcaaccgcgt gcagagtggc accagttggt agcggatttg cagcgcgaat tcccgtgtcc
     5341 aatcccgaaa gcgtgcgatc cattaagcca ttacggcctg atcaacgccg ttgccgcctg
     5401 tgtcgatgac aatgcgatta tcaccaccga tgtgggtcag catcagatgt ggaccgcgca
     5461 agcttatccg ctcaatcgcc cacgccagtg gctgacctcc ggtgggctgg gcacgatggg
     5521 ttttggcctg cctgcggcga ttggcgcggc gctggcgaac ccggatcgca aagtgttgtg
     5581 tttctccggc gacggcagcc tgatgatgaa tattcaggag atggcgaccg ccagtgaaaa
     5641 tcagctggat gtcaaaatca ttctgatgaa caacgaagcg ctggggctgg tgcatcagca
     5701 acagagtctg ttctacgagc aaggcgtttt tgccgccacc tatccgggca aaatcaactt
     5761 tatgcagatt gccgccggat tcggcctcga aacctgtgat ttgaataacg aagccgatcc
     5821 gcaggctgca ttgcaggata tcatcaatcg ccctggcccg gcgctgatcc atgtgcgcat
     5881 tgatgccgaa gaaaaagttt acccgatggt gccgccaggt gcggcgaata ctgaaatggt
     5941 gggggaataa gccatgcaaa acacaactca taacaacgta attctggagc tcaccgttcg
     6001 caaccatccg ggcgtaatga cccacgtttg tggccttttt gcccgccgcg cttttaacgt
     6061 tgaaggcatt ctttgtctgc cgattcagga cagcgacaaa agccatatct ggctactggt
     6121 caatgacgac cagcgtctgg agcagatgat aagccaaatc gataagctgg aagatgttgt
     6181 gaaagtgcag cgtaatcagt ccgatccgac gatgtttaac aagatcgcgg tgttttttca
     6241 gtaaccgctc aaggcttgaa caacatcgcg cttatcgtta aggtaagcgc gtttttttta
     6301 cccgccagga caagaccatg atcaccgttg cccttataga cgatcacctc atcgtccgct
     6361 ccggctttgc gcagctgctg gggctggaac ctgatttgca ggtagttgcc gagtttggtt
     6421 cggggcgcga ggcgctggcg gggctgccgg ggcgcggtgt gcaggtgtgt atttgcgata
     6481 tctccatgcc cgatatctcc ggtctggagc tgctaagcca gctgccgaaa ggtatggcga
     6541 cgattatgct ctccgttcac gacagtcctg cgctggttga gcaggcgctt aacgcggggg
     6601 cacgcggctt tctttccaaa cgctgtagcc cggatgaact cattgctgcg gtgcatacgg
     6661 ttgccacggg cggctgttat ctgacgccgg atattgccat taaactggca tccggtcgtc
     6721 aggacccgct aaccaaacgt gaacgccagg tggcggaaaa actggcgcaa ggaatggcgg
     6781 tgaaagagat tgccgccgaa ctgggcttgt caccgaaaac ggtacacgtc catcgcgcca
     6841 atctgatgga aaaactgggc gtcagtaacg acgtagagct ggcgcgccgc atgtttgatg
     6901 gctggtgatg aagacgttgt tctcccgctt aattaccgtt attgcctgct tttttatctt
     6961 ctctgccgca tggttttgcc tgtggagtat cagcctgcat ctggttgagc gccctgatat
     7021 ggcggtgctg ttatttccgt ttggtctgcg tctggggcta atgctgcaat gcccgcgcgg
     7081 atactggccg gtgctgctgg gcgcggagtg gctgctgatt tactggctaa cgcaggcggt
     7141 cggtttaacc cattttccgt tattgatgat cggtagttta ctgacgttac tgcccgtagc
     7201 gctgatctcg cgctatcgcc atcagcgtga ctggcgcacc ttgctgttac agggggcggc
     7261 gttaacggcg gcggcgttgt tgcaatcgct gccctggctt tggcacggca aagagtcgtg
     7321 gaatgcgctg ttgctgactt taactggcgg cctgacgctg gccccgatat gtctggtgtt
     7381 ctggcactat ctcgccaata acacctggct gccgctcggc ccgtcactgg tttctcagcc
     7441 aatcaactgg cgcgggcgac atctggtctg gtacttgctg ctgtttgtta tcagtctctg
     7501 gctccagttg ggattgccgg acgaactgtc gcgctttacg ccattctgtc tggcgctgcc
     7561 gattatcgcg ctggcctggc actatggttg gcaaggggcg ctgattgcga cgttgatgaa
     7621 cgccatcgcg ctgatcgcca gtcaaacctg gcgcgatcat ccggtggatt tattgctctc
     7681 gctgctggtg caaagtctga cagggttgtt gcttggcgct ggcatccagc ggttgcgtga
     7741 acttaaccag tcgctgcaaa aggaactggc gcgcaatcag catctggctg aacggttgct
     7801 ggaaaccgaa gagagcgtgc gccgtgatgt ggcgcgtgag ctgcatgatg atatcggtca
     7861 gaccatcact gctattcgta ctcaggcggg cattgttcag cggctggcgg cagataacgc
     7921 cagcgtgaag cagagcgggc agctcatcga acaactatcg ctgggcgttt acgacgcggt
     7981 gcgccgtttg ttgggtcggt tacgtccgcg ccagttggat gatctcaccc tggagcaggc
     8041 catccgctca ctgatgcggg aaatggagct ggaagggcgc ggtattgtca gccatctcga
     8101 atggcgaatc gatgaatcag cgttaagcga aaaccagcgc gtgacgctgt ttcgtgtctg
     8161 ccaggaaggg ctgaacaaca ttgtgaaaca tgctgatgcc agcgcggtca ccctgcaagg
     8221 ctggcagcag gatgaacggt tgatgctggt tattgaagac gatggcagcg gtttgccgcc
     8281 gggttccggg caacaaggtt ttggcctcac cggaatgcgc gagcgcgtaa cggcgctggg
     8341 tggcacatta cacatttcct gtctgcacgg cacgcgtgtc agcgtttctc tacctcaacg
     8401 ctatgtctaa ggtttgatga tgttgccgtt tctgaaagcg cctgccgatg cgccattaat
     8461 gactgataaa tatgaaattg atgcccgcta tcgctactgg cgtcggcata ttctgctgac
     8521 catctggctg ggttacgcgc tgttttactt cacgcggaaa agttttaacg ccgccgtacc
     8581 agaaatcctt gctaacggcg tgctcagccg tagcgatatc ggcctgttag cgaccctgtt
     8641 ttacattacc tatggcgtgt cgaagtttgt ctccggcatt gtcagcgatc gctcaaatgc
     8701 ccgttatttt atggggatag ggcttatcgc cacgggcatt atcaacattc tgtttggctt
     8761 ctcgacgtcg ctatgggcgt ttgccgtact ctgggtgctg aacgcctttt tccagggctg
     8821 gggttcaccg gtgtgtgcgc gtctgttaac ggcctggtat tcacgtaccg agcgcggtgg
     8881 ttggtgggca ttatggaaca cggcgcataa cgtcggcggc gcactcattc ccattgtgat
     8941 ggcagcggct gcactacatt acggctggcg tgccgggatg atgattgctg gttgtatggc
     9001 gatcgtcgtg ggaatttttc tctgctggcg gctgcgcgat cgcccgcagg cgttaggttt
     9061 accggcggtc ggtgaatggc gacacgacgc gctggagata gctcaacaac aagaaggggc
     9121 agggctgacg cgtaaagaga tcctcaccaa atatgtgttg ctgaatccgt atatctggct
     9181 gctttcgttt tgctatgtgc tggtctatgt ggtccgggcg gcgatcaacg actggggcaa
     9241 tttgtatatg tccgagacgc tgggcgtcga tctggtcacg gcgaatacgg cagtgacgat
     9301 gtttgaactg ggtggattta tcggtgcgct ggtagcgggt tggggctcgg acaaattgtt
     9361 taacggcaac cgagggccga tgaatttgat tttcgccgcc ggaattttgc tttcagtcgg
     9421 ctccctgtgg ctgatgccat ttgccagcta cgtgatgcag gcaacctgct tcttcaccat
     9481 tggttttttt gtctttggcc cacagatgtt aatcggtatg gcggcggcag agtgttccca
     9541 caaagaggcg gcaggggcgg cgacggggtt tgtcggcttg tttgcttatc tgggggcgtc
     9601 gcttgctggt tggccgctgg cgaaagtact cgatacctgg cactggagcg gattttttgt
     9661 ggttatctct atcgccgccg ggatttccgc actgctgtta ctgccctttt tgaacgccca
     9721 gacaccgcgc gaagcgtgat gcatctcacc ttttcacttc atatccggca aaactaagaa
     9781 attttccagg ttttgcctgg acgctatctc aggcctgatt tgctgctgat ttttacaatg
     9841 catgcctcac gcaggtattc atttcaggag taacccatgc tggctttcct aaaccaggtt
     9901 cgcaagccga ccctggacct tccgctcgaa gtgcggcgca aaatgtggtt caaaccgttc
     9961 atgcaatcct acctggtggt ctttatcggc tacctgacga tgtacctgat tcgcaagaac
    10021 tttaacatcg cgcagaacga tatgatttcg acctacgggt tgagcatgac gcagctgggg
    10081 atgatcggcc tgggtttctc catcacttat ggcgtgggta aaacgttggt ttcatactac
    10141 gccgacggca aaaacaccaa acaattcctg ccgttcatgc tgatcctgtc tgccatttgt
    10201 atgctgggct tcagtgccag tatgggcagc ggctcggtta gcctgttcct gatgattgcc
    10261 ttctacgcct taagcggctt tttccagagt accggcggtt cgtgcagtta ctccaccatc
    10321 accaaatgga cgccgcgtcg taaacgcggg acattcctcg gtttctggaa tatttctcac
    10381 aaccttggcg gtgcaggcgc agcaggtgtg gcgctgttcg gggcaaatta cctgttcgat
    10441 ggccatgtca tcggcatgtt tatcttcccg tcgattatcg cgctgattgt cggttttatc
    10501 ggcctgcgtt acggcagcga ctccccagaa tcttatggcc tcggcaaagc tgaagaactg
    10561 ttcggcgagg agatcagcga agaggacaaa gagacagaat ctaccgatat gaccaagtgg
    10621 cagatctttg ttgagtatgt gctgaaaaac aaagtgatct ggctgctgtg cttcgccaac
    10681 attttcctct atgtggtacg tattggtatc gaccagtggt caaccgtata cgcgttccag
    10741 gaactgaaac tctctaaagc ggtggcgatt cagggcttta cgctgtttga agctggtgcg
    10801 ctggtcggta cgctgctgtg gggctggctc tctgacctgg cgaacggtcg ccgtggcctg
    10861 gtggcctgca tcgcgctggc gctgattatc gccacgctcg gtgtgtatca acatgccagc
    10921 aacgaatata tctatctggc ttctctcttt gcgttgggtt tcctggtctt tggcccgcaa
    10981 ttgttgattg gtgtggctgc tgttggcttt gtacctaaaa aagcgattgg cgctgccgat
    11041 ggtattaaag gcacctttgc ttacctgatt ggtgacagct ttgccaagtt aggtctggga
    11101 atgattgccg atgggacgcc ggtattcggc cttaccggct gggcaggcac cttcgccgca
    11161 cttgatatcg ccgcgattgg ttgtatctgc ctgatggcga tagtggcggt gatggaagaa
    11221 cgcaaaatcc gccgcgagaa aaaaattcag cagttgacag tggcataaac gtaactggtg
    11281 acttttgccc ggcatgacgc cgggctttt
//
LOCUS       DAJTNN010000132        11257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTNN010000132 DAJTNN010000000
VERSION     DAJTNN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.132"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            250..1227
                     /gene="csiD"
                     /locus_tag="OO150_003076"
     CDS             250..1227
                     /gene="csiD"
                     /locus_tag="OO150_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5517140.1"
                     /translation="MNALIAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKPLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1247..2515
                     /gene="lhgO"
                     /locus_tag="OO150_003077"
     CDS             1247..2515
                     /gene="lhgO"
                     /locus_tag="OO150_003077"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5517141.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFNDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRATRSVDALHAAFNQ"
     gene            2538..3973
                     /gene="gabD"
                     /locus_tag="OO150_003078"
                     /pseudo
     CDS             2538..3973
                     /gene="gabD"
                     /locus_tag="OO150_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            3987..5253
                     /gene="gabT"
                     /locus_tag="OO150_003079"
                     /pseudo
     CDS             3987..5253
                     /gene="gabT"
                     /locus_tag="OO150_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5491..6852
                     /gene="gabP"
                     /locus_tag="OO150_003080"
     CDS             5491..6852
                     /gene="gabP"
                     /locus_tag="OO150_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5517142.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYVPVKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTDLLAIGIICTVPIMAA
                     GIS"
     gene            6911..7573
                     /gene="csiR"
                     /locus_tag="OO150_003081"
     CDS             6911..7573
                     /gene="csiR"
                     /locus_tag="OO150_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5517143.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7574..8023)
                     /gene="kbp"
                     /locus_tag="OO150_003082"
     CDS             complement(7574..8023)
                     /gene="kbp"
                     /locus_tag="OO150_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5517144.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8107..8265)
                     /locus_tag="OO150_003083"
     CDS             complement(8107..8265)
                     /locus_tag="OO150_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5517145.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8448..8747
                     /locus_tag="OO150_003084"
     CDS             8448..8747
                     /locus_tag="OO150_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5517146.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8757..9281
                     /gene="ygaP"
                     /locus_tag="OO150_003085"
     CDS             8757..9281
                     /gene="ygaP"
                     /locus_tag="OO150_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5517147.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDRIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9328..9732)
                     /gene="stpA"
                     /locus_tag="OO150_003086"
     CDS             complement(9328..9732)
                     /gene="stpA"
                     /locus_tag="OO150_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5517148.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10400..10849
                     /gene="alaE"
                     /locus_tag="OO150_003087"
     CDS             10400..10849
                     /gene="alaE"
                     /locus_tag="OO150_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5517149.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(10886..11230)
                     /locus_tag="OO150_003088"
     CDS             complement(10886..11230)
                     /locus_tag="OO150_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5517150.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
BASE COUNT         2655 a         2851 c         2973 g         2778 t
ORIGIN      
        1 tttttatcga ccagataatc tgttctctaa tgttaactcc ccctaacctg ttgctttagt
       61 tattcatttc ctgtctcact ttgccttaat accctacgtt aaatgttact aatttgttgt
      121 ttttgatcac aataagaaaa caatatgtcg cttttgtgcg catttttcag aaatgtagat
      181 atttttagat tatggctacg aaatgagcat cgccatgtca ccctacatct cataagagga
      241 tcgcttctga tgaatgcact gatcgccgta caaaataacg ctgtcgattc aggccaggac
      301 tatagcggat tcactctcat cccgtcggcg caatccccgc gtctgctgga actcaccttc
      361 accgaacaga cgaccaaaca gtttctcgaa caggttgccg aatggcccgt gcaggcgctg
      421 gagtacaaat cgtttctgcg ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaat
      481 cagctgcaac cattgctgtt gaagcccctg ctaaaccgtg ctgaaggtgc gctgttgatc
      541 aatgcggtgg gtatcgatga tgtggcgcag gcagatgaga tggtgaagct ggcaacggcg
      601 gtagcgcatc tgattggtcg ctcaaatttc gatgcgatga gcggtcagta ttacgcgcgt
      661 ttcgtggtga aaaatgtcga taactcagac agctatctgc gtcagccgca ccgcgtgatg
      721 gaactgcaca acgacggcac gtatgtcgaa gagatcaccg attacgtgct gatgatgaaa
      781 atcgatgagc aaaacatgca gggtggaaat tcgttgctgc tgcatctcga tgactgggaa
      841 catctggacc actatttccg ccatccactg gcgcgtcgcc cgatgcgctt tgccgcgccg
      901 ccgagcaaaa acgtcagcaa agatgttttc catccagtgt tcgacgttga tcaacagggt
      961 cgcccggtga tgcgctatat cgaccagttc gtccagccaa aagattttga agaaggcgtg
     1021 tggttgagcg agctttccga tgccattgaa accagcaaag gcattctttc tgtacccgtt
     1081 cccgttggca aattcctgtt gataaacaac ctgttctggc tgcacggtcg cgaccgcttt
     1141 actccgcacc cggatctgcg ccgtgaactg atgcgtcagc gtggctattt cgcttacgcc
     1201 actcaccact accagacgca tcagtaagcg caaaggaatt gagcggatgt atgattttgt
     1261 gattattggc ggcggcatca tcggcatgtc gaccgccatg caactgattg atgtttatcc
     1321 ggatgcccgc attgcgttgc tggaaaaaga gtccggcccg gcctgtcacc agacgggcca
     1381 caacagcggc gtgatccatg ccggggtcta ttacacgccc ggcagcctga aggcgcagtt
     1441 ttgcctggcg ggaaaccgcg ccaccaaagc cttttgcgat caaaacgaca ttcgctatga
     1501 caactgcggc aagatgctga tcgccacgtc cgatctcgaa atggaacgga tgcgcgcgtt
     1561 gtgggaacgc acggcggcga acggtatcga gcgcgagtgg ttaaacgccg aggaactgcg
     1621 cgagcgcgaa ccgaatatca ccgggctcgg cggcattttt gtgccgtcca gcggcattgt
     1681 cagctatcgc gaagtcacag cggcaatggc aaaaatcttc caggccagag gcggtgagat
     1741 tatttataac gccgaagtca gcgccctcag tgagcataaa aacggcgtgg tcgtccgaac
     1801 ccgtcagggc ggcgaatatg aagcatcgac gctgattagc tgttccgggc tgatggctaa
     1861 ccggctggtg aaaatgctcg gcctcgaacc gggttttatt atctgcccgt tccgtggcga
     1921 gtatttccgc cttgcgccgg agcataacca gattgttaac catctgattt accccatccc
     1981 tgacccggca atgccgtttt tgggcgtgca tctcacccgc atgatcgacg gcagcgtgac
     2041 cgtcgggcca aacgcggtgc tggcttttaa acgcgaaggc tatcgcaagc gcgacttctc
     2101 atttaacgac acgctggaaa ttttaggctc gtcggggatt cgccgggtgc tgcaaaacca
     2161 tctacgctca ggactgggcg agatgaaaaa ctcgctgtgc aaaagcggct atctgcggct
     2221 ggtgcaaaag tattgtcccc ggctttcgtt aagcgatctc cagccctggc ccgccggtgt
     2281 gcgggcgcag gcggtatcgc cggacggcaa gctgattgac gattttctgt ttgtcaccac
     2341 cccgcgcacg atccacacct gcaatgcgcc ctccccggca gcgacatcag caattcctat
     2401 tggtgcgcat attgtcagca aggtacaaac gctgttggca agccagagta accccggacg
     2461 cacgctgcga gcgacacgta gtgtggatgc cttacacgcc gcatttaatc aataaccttt
     2521 gaaaacagga cgtaacaatg aaacttaacg acagtaactt attccgccag caggcgttga
     2581 ttaacgggga atggctggac gccaacaatg gcgggatcat cgacgtcacc aatccggcga
     2641 acggcgacaa gctgggtagc gtgccgaaaa tgggcgctga tgaaacccgc gccgctatcg
     2701 acgccgccaa ccgcgccctg cccgccaggc gtgcgctcac cgccaaagaa cgcgccaaca
     2761 ttctgcgcaa ctggttcaat ttgatgatgg agcatcagga cgatttagcg cgcctgatga
     2821 ccctcgaaca gggcaaacca ctggccgaag cgaaaggcga aatcagctac gctgcctctt
     2881 ttattgagtg gtttgccgaa gaaggcaaac gtatttatgg cgataccatt cccggtcatc
     2941 aggccgataa gcgcctgatt gttatcaagc agccgattgg cgttaccgca gctatcacgc
     3001 cgtggaactt cccggcggcg atgattaccc gtaaagccgg tccggcgctg gcggcaggct
     3061 gcaccatggt gctgaagccc gccagtcaga caccgttctc tgcgctggcg ctggcggagc
     3121 tggcgattcg cgcgggcatt gcggctggcg tgtttaacgt ggtcaccggt tcggcaggcg
     3181 cggtcggtaa tgaactgacc agcaacccgc tggtacgcaa actgtcgttt accggttcga
     3241 ccgaaattgg ccgacagtta atggaacagt gcgcgaaaga catcaaaaaa gtgtcgctgg
     3301 agctgggcgg caacgcgccg tttatcgtct ttgacgatgc cgatctcgat aaagccgtgg
     3361 aaggcgcgct ggcctcgaaa ttccgcaacg ccgggcaaac ctgcgtctgc gccaaccgtt
     3421 tggcgtgtat gaccgctttg ccgaaaaatt gcagcaggcg gtgagcaaac tgcacatcgg
     3481 cgacgggctg gataaaggcg tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa
     3541 agtggaagag catattgccg atgcgctgga gaaaggtgcg cgtgtggttt gcggcgctaa
     3601 agcggatgaa cgcggcggca acttcttcca gccgaccatt ctggtggacg tgccggccaa
     3661 cgccaaagtg tcgaaagaag agacgttcgg ccccctcgcc ccgctgttcc gctttaaaga
     3721 tgaagccgat gtgattgcgc aagccaatga caccgagttt ggccttgccg cctatttcta
     3781 cgcccgtgat ttaagccgcg tcttccgcgt gggcgaagcg ctggagtacg gcatcgtcgg
     3841 catcaatacc ggcattattt ccaatgaagt ggccccgttc ggcggcatca aagcctcggg
     3901 tctgggtcgt gaaggttcga agtatggcat cgaagattac ttagaaatca aatatatgtg
     3961 catcggtctt taactggaga atgcgaatga acagcaataa agagttaatg cagcgccgca
     4021 gtcaggcgat tccccgtggc gttgggcaaa ttcacccgat tttcgctgac cgcgcggaaa
     4081 actgccgggt gtgggacgtt gaaggccgtg agtatcttga tttcgcgggc gggattgcgg
     4141 tgctcaatac cgggcacctg catccgaagg tggtggccgc ggtggaagcg cagttgaaaa
     4201 aactgtcgca cacctgcttc caagtgctgg cttacgagcc gtatctggag ctgtgcgaga
     4261 ttatgaatca gaaggtgccg ggcgatttcg ccaagaaaac gctgctggtt acgaccggtt
     4321 ccgaagcggt ggaaaacgcg gtaaaaatcg cccgcgccgc caccaaacgt agcggcacca
     4381 tcgcttttag cggcgcgtat cacgggcgca cgcattacac gctggcgctg accggcaagg
     4441 tgaatccgta ctctgcgggc atggggctga tgccgggtca tgtttatcgc gcgctttatc
     4501 cttgcccgct gcacggcata agcgaggatg acgctatcgc cagcatccac cggatcttca
     4561 aaaatgatgc cgcgccggaa gatatcgccg ccatcgtgat tgagccggtt cagggcgaag
     4621 gcggtttcta cgccgcgtcg ccagccttta tgcagcgttt acgcgctctg tgtgacgagc
     4681 acgggatcat gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt
     4741 ttgcggtgga gcagatgggc gtggcgccgg atcttaccac ctttgcgaaa tcgatcgctg
     4801 gcgggcgtca ccgggcgcgc ggaagtgatg gacgctgttg caccgggcgg tctgggcggc
     4861 acctatgccg gtaacccgat tgcctgcgtg gctgcgctgg aagtgttgaa ggtgttcgag
     4921 caggaaaatc tgctgcaaaa agccaacgat ctggggcaga agctaaaaga cggattgttg
     4981 gcgatagccg aaaaacaccc ggagatcggc gacgtacgcg ggctgggagc gatgattgcc
     5041 atcgagctgt ttgaagatgg cgatcacagc aaaccggacg caaaactcac cgccgggatc
     5101 gtggcacgcg cccgcgataa gggcctgatt cttctctcct gcggcccgta ttacaacgtg
     5161 ctgcgcatcc ttgtactgct caccattgaa gacgctcaga tccgtcaggg tctggagatt
     5221 atcggtcagt gttttgatga ggcgaagcag tagcaccgtc cccatgcagg aggcgacgct
     5281 gcgcgtcttg tccggcctac gagaatccag gtcggataag gcgtttacgc cgcatccgac
     5341 aatctgtacg tgaacaggaa taaatctatg tcggccggat aatgcggagc cgctctccgg
     5401 caaaaagaat caataacaat taaacgcgtg acccggcgcg ggaaatgtcg gggcgctctc
     5461 ccaagtgaca cactttcgag aggattcagg atggggcaat catcgcaacc acatgagtta
     5521 ggcggcgggc tgaaatcacg ccacgtcacc atgttgtcta ttgccggtgt tatcggcgca
     5581 agtctgtttg tcggttccag cgtcgccatc gccgaagcgg gcccggcagt attactggcc
     5641 tatctgttcg ccgggctact ggtggttatg attatgcgga tgttggcgga aatggcggtt
     5701 gccacgcccg ataccggttc gttttccacc tatgccgata aagccattgg tcgctgggcg
     5761 ggctatacca tcggctggtt gtactggtgg ttttgggtac tggttatccc gctggaagcc
     5821 aacatcgccg ccatgatcct gcactcgtgg gttccaggca ttcccatctg gttattttcc
     5881 ctcgtcatta cccttgcctt aactggcagt aatttattaa gcgttaaaaa ctacggcgaa
     5941 tttgagttct ggctggcgct gtgcaaagtc atcgctatcc tggcctttat tttccttggt
     6001 gcagtcgcaa ttagcggttt ttacccttat gccgaagtga gcgggatctc aagattgtgg
     6061 gatagcggcg gctttatgcc caacggcttc ggtgcggtat taagcgcgat gttgatcacc
     6121 atgttctcgt ttatgggcgc agaaattgtc accattgccg ccgcggaatc cgacacgccg
     6181 gaaaaacata ttgtccgcgc cactaactcg gttatctggc gtatttctat cttctatttg
     6241 tgctctattt ttgtcgtcgt ggcattaatt ccgtggaata tgcccggact aaaagccgtc
     6301 ggttcttatc gctcggtact ggaattgctc aatattcccc atgcgaaatt aatcatggac
     6361 tgtgtgatat tactttccgt aaccagctgc ctgaactcgg cgctgtatac cgcttccagg
     6421 atgctctact ccttaagccg tcgcggagat gcgcctgcgg taatgggtaa aatcaaccgc
     6481 agtaaaaccc cgtacgtggc ggtgttactc tccaccggcg cggcattctt aacggtggtg
     6541 gtgaactatt acgtgcctgt gaaggtattt aaatttctga tcgacagctc cggcgctatc
     6601 gccctgctgg tttatttagt catcgccgtt tcacagttgc ggatgcgcaa aattctgcga
     6661 gcagaaggaa gcgaaattcg cttgcgcatg tggctttatc cctggctcac ctggctggtc
     6721 atcggcttta ttacctttgt gttggtagtg atgctattcc gcccggcgca acagttagaa
     6781 gtgatctcca ccgacttatt agcaataggg attatctgta ccgtgccaat tatggcggct
     6841 ggaataagct gataatgtgg caaaaaacac ccgttcataa tacgcgctga taataatcag
     6901 gagtcacacc atgaccatta cgtctctgga tggctatcgc tggctgaaga acgatattat
     6961 tcgcggtaat tttcaaccgg atgaaaaatt acggatgagt ttgctgacat cgcgttatgc
     7021 acttggcgtt gggccgttgc gggaagcact ttctcaactg gtggcggaac ggctggtcac
     7081 ggtggtgaat caaaaagggt atcgggtggc gtctatgtca gagcaggagc tgctcgatat
     7141 tttcgatgcc cgcgccaata tggaagcgat gttagtgagt ctggcgattg cccgcggtag
     7201 cgatgagtgg gaggcagacg ttctcgcaaa agcgcatctg ctcagtaagc tggaagcctg
     7261 tgacgccagc gagaaaatgc ttgatgagtg ggatctgcgt catcaggcgt ttcatacagc
     7321 aattgtggcg ggctgtggtt ctcactattt gctgcaaatg cgtgaacggt tgtttgatct
     7381 ggcggcgcgc tatcgattta tctggctgcg gcgaacggtg ctttcggtag aaatgctgga
     7441 ggataaacac gatcagcacc agaccctgac tgcggcggta ctggcgcgag ataccgcgcg
     7501 cgccagtgag ttaatgcgcc agcatttact gacgccaatt cccattatcc agcaggcgat
     7561 ggctggcaat taactactct tccggaatac gcaacacttg ccccggataa attttatccg
     7621 ggctttttag catcggttta ttcgcttcga agattttatt gtacagatta gcgttaccgt
     7681 agacctgttt ggaaatggca ctcagagtgt cgccagactt aacggtataa aactggctgg
     7741 cagtggctgg tgtcgccgtt ttcacctgat catcaacact ggcaataccg gaaatattcc
     7801 ccaccgcaac aaggattttc tccttcgcct cctgactcag gccgtcacca gtgaccgtcg
     7861 ctttgccgtc ggcaatttga atgttcactt tatcggcatc aggtataccg gttttgctca
     7921 gatgctcctg caccttcttc gcctgatcgt ctttatcgtg ctgacctgta accgcgtccc
     7981 agagtttttc tccggcatct ttcacaaaat tgaacagacc catagctacc tcattcgtta
     8041 acggaaacac tcagaaagtg tagcagagga acgcggcgcg ttaacgccgc gggggggagc
     8101 acaggtttaa tcgcgggttt gcacccagaa cgcgtgaatc aaaccgggga tatacccaag
     8161 cagtgtcagc agaatattaa taatgaacgc ccaaccgaac cctttaccga gcagcacgcc
     8221 gagcggcggc agaatgatgg tgatgacgat tctccagaaa cccatatgta ctccctataa
     8281 gaaaattact cattgtttaa aaagagattt tatctcttaa gcgtagtaat tttagcggag
     8341 cctgccagtt tcagcttacg cttttactct catttacgct gctgaggctg gtatatgttg
     8401 catttttagt ttagagttta cttaatttag aaaatactta aataaatatg actgaactcg
     8461 cgcaattaca ggccagtgcc gaacaggcag cggccttatt gaaagcaatg agccatccta
     8521 aacgattgct gattctgtgc atgcttagcg gttcccccgg caccagcgcg ggagagctga
     8581 cgcgcattac cggactgagt gcctctgcga catcacagca tctcgctcgt atgcgggacg
     8641 aagggcttat cgacagccaa cgggatgccc aacgcattct atattccatt aaaaatgagg
     8701 cggtaaatgc cattatcgcc accctgaaaa atgtctattg tccgtaagga gtcactatgg
     8761 ctttgacaac catttcgccg catgatgcac aagaattaat cgcacgcggc gcaaagttaa
     8821 tcgatattcg tgacgctgat gaatatcttc gtgaacatat tcctgaagca gatctggctc
     8881 cattatccgt gctggaacag tcaggtcttc cacctaaatt acgtcgcgag caaattatat
     8941 tccactgcca ggcaggtaaa cgcaccagta ataacgccga taaattagca gcgattgccg
     9001 cccccgcaga aatattttta ctcgaagata ggattgatgg ctggaaaaga gctggattgc
     9061 cagtagcggt aaataaatct caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg
     9121 gtggtttaat attaatcggc gttgtactgg gttataccgt aaatagcggt ttcttcttat
     9181 taagtggctt tgttggtgcc gggttactgt ttgcaggaat cagcggtttt tgtggaatgg
     9241 caaggttgtt agataagatg ccgtggaacc aacgagcttg agaagcgacg ccggacgcgc
     9301 cctagcagcg acatccggcc tcagtaatta gatcaggaaa tcgtcgagag atttaccttc
     9361 tgccagcgcc tgagcaattg gcttcggtgt acggccctga ccggtccagg tcttagtttc
     9421 accgttaaca tcagtgaatt tatatttcgc cggacgcggc tggcgttttt taccagcacg
     9481 tggtgcagca gcagagctat tacccaataa ctcttccggg ttaattccgt cagctttcat
     9541 cagctccagc caggtgctaa ttttttcctg acgctcagcc agttcacgct gctgctgttc
     9601 ttcttcttca cgtctttctt tagtgacaac cctgaatttt tcgagcattt cttcaagaac
     9661 gtcaatggag aattcgcgag ccatcgcacg gagggttcga atgttattta aactttgtaa
     9721 cattacggac ataataaaga aaacctttta acgccaaaac aaaaaagtat ttcgtcgcgt
     9781 agtgtaagcg atcacttatt atatttccac aaaatttaat ctatttacag tcctgcgcga
     9841 gattatttca gctgttattt tctgaaactc tcagaataat ttcaagatta tatattagcc
     9901 attttgatga gtaatttagg ttattggtga acagagtggc gctctctgct cgctaactcc
     9961 gcaagcctga taaaaccgaa gtgaataaaa atgcaatata acagaataaa agaaaacacc
    10021 cctcactggt gctttctgct gttaatgtac attttctggc atcaaaaatg ctgtaaatga
    10081 cccggtcacg acaaacaccg aattctctac gcttatagcg ttacctcacc cccaaacttt
    10141 cattagtgaa attaaaggca aggtcatatc caattgattt aattgaaaat aaatcatatc
    10201 ggcatgtaaa aatccacaat gtacaaaaaa cgagctgtta cggaatattt tatctacaaa
    10261 aaactgacta aataaaagtt tttcactaat tgattagtca tagccagcga tatacgctat
    10321 gcgaaaatgc agatggcaat gagatccact gctttcatct ccattaacat cccattacgc
    10381 tttattaagg agcattagca tgttctcacc gcagtcacgc ttgcgtcatg cagttgcaga
    10441 tacgttcgcg atggttgttt actgttctgt cgtgaacatg tgtattgaag ttttcctctc
    10501 cggaatgagc ttcgaacagt ctttttattc cagattggta gcgattccgg tgaacatctt
    10561 aattgcatgg ccatacggta tgtaccgcga tctgtttatg cgcgcggcac gcaaagttag
    10621 cccgtcgggc tggataaaaa atctggctga tatcctggct tatgtgacgt tccagtcacc
    10681 ggtgtatgtg gcgatcttgt tagtggtggg cgcagactgg catcagatta tggcggcggt
    10741 cagttcaaac atcgttgttt cgatgttgat gggggcggtt tatggctact tcctcgatta
    10801 ttgccgccga ctgtttaaag tcagccgtta ccagcaggta aaagcctgac tcttcttttc
    10861 gcgactggca tcgccagtcg cgtctttaac tggcttcgcc aaataaacca ttcaaataac
    10921 gttcaagcgc aacacgcgaa ctgaagccat gcgggatacc gtaatgctcg tgtcgctcgc
    10981 ctgctaaata gagcggaaat tgctggtaat gatcgcaagt tttaatatcg gaagcgggtt
    11041 cagccagtgt tgaactgcgt tcttttaatg ttggatgaat taccagagcg gtacgtccca
    11101 ttcgcgcttc gcgattaaca tagacataat tttccccacg gcggtaacca tacgctttct
    11161 gggttaccac atcgacagta aaaccgactt tttcaagtac gcgcgccacc tcgtctggtc
    11221 gtaaatacat atttgatcct cgttattttc aaccgcg
//
LOCUS       DAJTNN010000133        11186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTNN010000133 DAJTNN010000000
VERSION     DAJTNN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.133"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            312..1187
                     /locus_tag="OO150_003089"
     CDS             312..1187
                     /locus_tag="OO150_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR5517151.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKNMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1195..1971
                     /locus_tag="OO150_003090"
     CDS             1195..1971
                     /locus_tag="OO150_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR5517152.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2140..4401
                     /locus_tag="OO150_003091"
     CDS             2140..4401
                     /locus_tag="OO150_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5517153.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4470..5645
                     /gene="senB"
                     /locus_tag="OO150_003092"
     CDS             4470..5645
                     /gene="senB"
                     /locus_tag="OO150_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR5517154.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            6104..6454
                     /locus_tag="OO150_003093"
     CDS             6104..6454
                     /locus_tag="OO150_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5517155.1"
                     /translation="MPSGRHTARIRTGHDSGTVSPFVHSTLSPLVPVYPVFITVIADH
                     DKPQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEELSNLVLLHPNCHR
                     QLHSCEAGSLKKGL"
     gene            6597..6818
                     /locus_tag="OO150_003094"
                     /pseudo
     CDS             6597..6818
                     /locus_tag="OO150_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6839..6901)
                     /locus_tag="OO150_003095"
                     /pseudo
     CDS             complement(6839..6901)
                     /locus_tag="OO150_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            7352..8764
                     /gene="ospC1"
                     /locus_tag="OO150_003096"
     CDS             7352..8764
                     /gene="ospC1"
                     /locus_tag="OO150_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5517156.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(8868..9016)
                     /locus_tag="OO150_003097"
                     /pseudo
     CDS             complement(8868..9016)
                     /locus_tag="OO150_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            9092..10789
                     /gene="ospD3"
                     /locus_tag="OO150_003098"
     CDS             9092..10789
                     /gene="ospD3"
                     /locus_tag="OO150_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5517157.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGKLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKI"
     gene            10906..>11186
                     /locus_tag="OO150_003099"
     CDS             10906..>11186
                     /locus_tag="OO150_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5517158.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRRP"
BASE COUNT         3447 a         2386 c         2370 g         2983 t
ORIGIN      
        1 ttcctcccgc cggccatttt cttcgacatg tacatcaggc gcggaactca tcggggcgcg
       61 ggaatgaaat cccattcaga ctccggatag attcaagcaa gcccacttat cgtcaaaatc
      121 ggtgttgcaa aaacgggagt gaccatagat ccgttttcca agcggacccc ataatatcgg
      181 ctggtaaaca tcactcgcta gtgcattctt tttgttggta atgataatta tttacattta
      241 ttctttaatg ttttatcata attaatgttt tatcaataaa tatatggaaa tatattgttt
      301 cagatgggaa tatgagaaag ttcatcctta tttctatgat aacgttagct agtgttagtc
      361 tgggagcctg cagacagaat gtcaccataa agcaggatgc gccagggcag aaagctttcc
      421 tgattgacgg gcagatttac gatttgcaca gtggcaaaat tatttctagc agcgagcttt
      481 tggcggatct tgctaccgct cgacatctta ttattggtga aaaacacgat aatgccgagc
      541 atcatcagat tgagctttgg cttattcaaa atctgctcat ccagcgacca caggggagtg
      601 tgttgcttga gatgttgacg tcagaccaac agccccgagt taaccaagtt aaatgttggc
      661 tgaaagataa tcctgtggtt cgagatagcc gtgttcagga actacttaac tggcaaaaag
      721 ggtggtcctg ggagatgtat ggcgacatcg tcatgcagct attacgtggg ccttacccgc
      781 tgctcaatgc caacattggc cgggagcaga tgttggcact gtacaaaaaa aacgaatttc
      841 ctaaaggaaa aaaatccact gcacctgtgg tacaagaagc tctgcgtgaa accatcatag
      901 ctatgcatga aggcaacctt gaaagccagc aactgacgtc aatgctctct gttcagcagc
      961 agcgggatcg ctacatggcc cgccagcttc tgagtgcccc ggtaccgtcg ttgttgattg
     1021 caggtggtta tcacgcgtca aaaaacatgg gcgtaccgtt acacatggaa gatcttgcca
     1081 ccggcaccca tccagtggtg ttgatgcttg cagaaaaagg aatgaacatc acagtagatc
     1141 atgctgacta cgtgtggttt gtagcgccag atacaacaaa aaggtaaacc atggatgatg
     1201 aatattctcc acttcccaca gtcggtaaaa tggagcagct ggtttatctg ttctctactg
     1261 cttcatggac tgattttcct tacgtttatc tggcgttttt ccgaagttca gcccgctatg
     1321 tctcctgcac cggcgatcat gctgcaatgg gcagaaatta tagaggctcc ttcttctcca
     1381 ctttcgcttc cggtgggaat cgctcaacag gagtccgcag tgacagaaga aaagcaacaa
     1441 actgaggaca gacaacaacg tcctgtcact gaagacagtg atgcaaccat tgaaataact
     1501 aggaagaaaa agtcatccga tggagaaaag aaaaagacac gtccacctcg taagataaag
     1561 gctcaaacca gcgacagcaa ccccacagcc gtttccagta atgctgcacc tcaggctctg
     1621 gtcgaatcgt cccgtattgc tgcgcctttt aacagtgact caacaaagcg agataattct
     1681 gaggcatctt gggaaagcag agtaaaaggc caccttaacc gttataaacg ttaccctggc
     1741 gatgccagaa aacgtgcacg aaccggcaca gccgttgtga cttttaccgt aaacacggaa
     1801 ggcactattg tcagtagttt cttggaaatc tcgtcaggaa ccctttctct tgaccgcgaa
     1861 gctattgctg ttttagaacg cgctcaacca cttcccaaac caccgcctga aatattggag
     1921 ggaggattat ttaaagtaaa aatgccgatt acctttaagt tgaaggagta aagggataat
     1981 tggccgttca agttcattta actgaaattt aattttcatt tttatgctga acattttatc
     2041 aaacttttcc actgtcgaaa aaatcaaaaa gacagtagat cataattaat aaacaaatag
     2101 ttatacttat ctttattaat attatatcca aggaaattta tgaacgttat aaaactggct
     2161 atcggctcag gcatattatt gctcagctgc ggtgcttact cacaatccat cagtgaaaaa
     2221 actaattccg acaaaaaagg agcggcagaa ttcagtccgc tcagcgtttc tgtcgggaag
     2281 acgaccagtg agcaggaagc tctcgagaaa acaggcgcga ccagttcccg gacaacggac
     2341 aaaaacctgc aatcacttga cgcaacagtg cgtagtatgc ctggtactta tactcaaata
     2401 gatcctggtc agggagcaat cagtgtgaat attcgaggca tgagcggatt tggtcgtgta
     2461 aacactatgg tcgatggtat tacccagagt ttttacggaa cctctacctc cggaacaacg
     2521 gcgcatggtt caactaacaa tatggctggc gtacttatag atcctaactt actggtagca
     2581 gttgatgtta cacgcggtga cagcagtggc tctgaaggga tcaacgccct tgccggtagt
     2641 gcaaatatgc gtactattgg cgttgacgat gtaatattta acggtaatac atatggcctt
     2701 cgttcacgtt tctctgtcgg tagtaatggg ctgggacgca gcggaatgat cgcccttggt
     2761 ggaaaaagcg acgcttttac ggatacggga agcattggcg ttatggctgc tgtgagtggc
     2821 agttctgtgt actctaattt ctcaaatggt tctggaatta acagcaaaga gtttggttat
     2881 gataaatata tgaagcagaa ccccaaatcc caactgtata aaatggatat cagaccagac
     2941 gaatttaaca gcttcgaact ttccgctcga acctatgaaa ataaatttac acgtcgtgat
     3001 ataaccagtg acgactatta cattaaatat cattacaccc ctttttctga attaattgac
     3061 tttaacgtaa cggccagtac cagtcgcggt aatcaaaagt atcgtgatgg ctcgctgtat
     3121 actttctaca aaacctcagc gcaaaatcgt tctgacgcgc tggatatcaa caataccagc
     3181 cggttcactg tcgcggacaa tgaactggag tttatgctgg gcagcaaact gatgcgtacc
     3241 cgctatgacc ggaccattca ctcagcggcg ggcgacccga aagcgaatca ggaatcgatc
     3301 gagaacaatc cgttcgcacc ctccggccag caggatattt cagcgctgta taccgggctg
     3361 aaggttacgc gcggcatctg ggaggcagat ttcaatctca actacacacg taacaggatc
     3421 acagggtaca agcccgcctg cgattcacgc gttatctgcg tgccacaggg tagctacgat
     3481 attgacgata aagagggtgg cttcaaccct tcagttcagc tttctgctca ggtaacacca
     3541 tggcttcagc cgttcattgg ctacagcaaa tccatgcgcg ccccgaacat ccaggagatg
     3601 ttcttctcta attcaggagg cgcatccatg aacccattcc tgaagcctga acgtgcagaa
     3661 acctggcagg cgggttttaa cattgatacc agagatttac tggtcgaaca ggatgccctg
     3721 cgctttaagg ctctggcgta ccgcagcagg atccagaact acatctacag cgagtcttat
     3781 ctggtttgtt ctggaggtcg taaatgcagt atggctgagg tgattggcaa tgactgggag
     3841 ggcattagcg atgaatacag cgacaatatg tacatctacg ttaactcggc aagcgacgtt
     3901 attgcaaagg gcttcgaact ggagatggat tatgatgcag gttttgcttt tggccgactc
     3961 tctttcagcc agcagcaaac agaccagcca acctccatcg ccagcaccta ctttggcgca
     4021 ggggatatga ccgaactgcc cagaaaatac atgacgctgg atactggtgt tcgcttcttc
     4081 gataacgcgt tgaccctggg cactatcata aaatacacag gcaaggctcg tcgcctgtcg
     4141 cctgattttg agcaggacga acataccggc gcaataatca aacaggattt gccgcagatc
     4201 ccaacgatta tcgatctcta tggtacttac gagtacaacc gcaacctgac actgaaactt
     4261 tcggtacaaa acctgatgaa cagagattat tcggaggcgc tgaataagct caacatgatg
     4321 ccaggtcttg gtgacgagag ccacccagcc aattccgcgc gtggcagaac atggatattt
     4381 ggcggggaca ttcgtttctg atgacaattt ccgccaggtt cagacaatac gttttctctc
     4441 ttatgtcaat tttattgcag gaacgaaaaa tgaatatttt cactttatcc aaagcaccgc
     4501 tatacttgtt aatttcacta tttttaccca cgatggccat ggctatcgat ccacctgaac
     4561 gcgaactttc gcgatttgcc ctgaaaacga attaccttca gtcccctgat gaaggcgtct
     4621 atgaactggc gtttgataat gccagtaaaa aggtgtttgc agcagtcacc gatcgtgtaa
     4681 atcgtgaagc caataaaggc tatctgtatt cgtttaattc agattcgctg aaagtcgaaa
     4741 ataaatacac gatgccatac cgggcatttt cgctggcgat aaatcaggat aaacatcagc
     4801 tctatatcgg acacacccag tcagcgtccc tgcgtatcag tatgtttgac accccaaccg
     4861 gtaaactggt aagaaccagc gacaggttaa gttttaaagc ggcaaacgct gcagattcgc
     4921 gttttgagca tttgcgccat atggtttaca gccaggattc cgataccctg tttgtgagtt
     4981 atagcaatat gctgaaaacg gccgagggca tgaagcctct gcataagctg ttaatgctcg
     5041 acgggacgac gcttgcctta aaaggcgagg ttaaggatgc ttacaaaggt acagcgtatg
     5101 gtctgacgat ggatgaaaaa acacagaaaa tctacgttgg cggaagagat tacatcaacg
     5161 aaattgatgc gaaaaatcag acgctgctgc gtaccatccc gttgaaagat ccgagaccac
     5221 aaatcacaag tgtgcagaat ctggcggtgg actccgcttc tgaccgtgcc tttgtggtgg
     5281 tattcgacca tgacgatcgt tccggtacaa aagatggcct ctatattttt gacttacgcg
     5341 acggtaaaca gcttggctat gtgcacacag gagccggagc taacgcggtg aaatacaatc
     5401 cgaaatataa cgaactgtat gtcaccaact tcactagcgg caccatcagc gtagtggatg
     5461 ccaccaaata cagcatcacc cgtgaattta acatgccggt ctacccaaac cagatggtgt
     5521 tgtcggacga tatggatacc ctttacattg gcatcaaaga aggctttaac cgcgattggg
     5581 atcctgatgt gtttgtggaa ggagctaaag aacgtattct gagcattgat ttgaaaaagt
     5641 cgtgatcggg ggaaaaaaca tgggataaaa actccgttat tccactaaac tggccggact
     5701 aggaaaagca aacatctctg tcctgccggt acgcctgtta cgccgctcca tgaggctgag
     5761 gaatctccag aaaacgagac aggcacagca ttgtacgatc aactgatttg tgccaataat
     5821 tcaatgaaat cacagctatg cctgttcacg tataggaaat tgaaaaacta agcgatacca
     5881 ctgtccgcgt cttatgcctc gtgcggtaag ctgtttttca catcacgccg gtgttttttt
     5941 ctttctgtgg acggttttaa tactgtaatc ggccgttttt tatacttacc ccgattcccg
     6001 ggcgcactta tcctgcatca ccccgggata tacctcagca gactgatatc ccgcgcgttt
     6061 ttcttcagcc cctccacatt cagtcccgcg atggcccggc ggtataccat ccggccgcca
     6121 cacagcacgc attcgaacgg gtcacgactc aggaactgtt tcaccatttg tgcatagcac
     6181 actttcgcca ctggttccgg tttatcctgt ttttataacg gtaattgcag accatgacaa
     6241 accgcagccg tcaggctgcc tactcgagtc acaaggctct ctgtgtccga tatgccggca
     6301 gcgcattacc catgaaactg gctggaacgt acaccataag gtgaagaaag ttatgggggg
     6361 cggtgaagaa ttatctaacc ttgtcttgtt acatccaaat tgccatcgac agttacacag
     6421 ttgtgaagcc ggctctctca aaaagggctt ataaaggctt gagcggtgta ccgggaaacc
     6481 ggtatgcacc gttcttaggg gagggcagtc cagcaatgaa ctgtccttac ccgacaaaca
     6541 tccaaaacga acggaacgtg ctggaatccg catataaccc gttgtaaagt gaggaaatgg
     6601 acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg caacaggaca
     6661 ctatccacca gatggctgaa tacaaccgcc tgctctcaca gcggatggcg gcttatgctt
     6721 ccgaaatcaa ccgactgaag gcgctggttg cgaaactgca gcgtatgcag ttcggtaaaa
     6781 gcccagaaaa acttcgcgca aaaaccgaac ggcagattcc attttcacgg gcgatctacg
     6841 ccacacaggc tctgggttgc agtgactgct ccattaaggc gattttgaac tcataaggga
     6901 agtaacaaaa aaactgcaaa agtacaataa ttatatatgg ccaaactaac ctctattaaa
     6961 aaaacatcag tttttcacac ttagttgagc ttcatgaaaa aatcatcgag gatttcattg
     7021 cagaatacac attccactaa ctggaagcac tccataagag gaatcagaga aagtatcgtt
     7081 tttttatagt aaaattcatt gctgttcaat aaaattgata taattattaa attatggaaa
     7141 taatttcaca aaaaagatta acttgagttc ttaagcatcg gattttagat attttgaaat
     7201 atctggcatc acataacatc aaaagtatat aataccctta tctaattaca tgcagtatat
     7261 tgtatatata ctcaaaaatt gtaattcata ccctactata tcttgtgggg aaagatatta
     7321 attaaaactg ttttcatata aggttcattt tatgaatata tcagaaacac tgaactcagc
     7381 aaatacccaa tgcaatatag attctatgga taacagatta catacattgt ttccaaaagt
     7441 gacatcagtg cgaaacgctg cacaacaaac tatgccagat gaaaaaaatt taaaagatag
     7501 tgcaaatatt attaaagatt tctttaggaa aactatagca gcacaaagtt atagtagaat
     7561 gttctctcaa ggctctaact ttaaatcttt aaatatagca attgatgcac catcagacgc
     7621 taaagcctca tttaaggcta ttgagcacct tgacagatta tcgaagcatt atatatctga
     7681 aataagggaa aaacttcatc ctctttctgc agaggaactc aatttgcttt cgctaattat
     7741 taattctgat ttaatcttca gacatcaaag taattctgat ttgtctgata aaattttaaa
     7801 cattaagtca ttcaataaaa ttcagtctga aggaatatgc acaaaacgaa acacatacgc
     7861 tgatgatata aaaaaaatag ctaatcatga ctttgtgttt tttggcgttg aaatctctag
     7921 ccatcagaaa aaacaccccc tgaatacaaa acatcacact gttgattttg gtgcaaatgc
     7981 gtatatcatt gatcatgact ctccatatgg atatatgaca ttaaccgatc actttgataa
     8041 tgctattcca cctgtttttt accatgagca ccaatcattt ttagataaat tttcagaggt
     8101 taataaagaa gttagtcgat acgtacatgg aagtaaagga attatagatg taccaatatt
     8161 caatactaaa gatatgaagt tagggctcgg attatacctg attgacttta ttagaaaaag
     8221 tgaagaccaa agcttcaagg agttttgcta tggaaaaaat cttgcccctg tggatctgga
     8281 tagaatcata aactttgttt ttcagccaga gtaccatata cctaggatgg taagtacaga
     8341 aaacttcaaa aaagttaaga ttagagaaat atccttagag gaggctgtta cagcatctaa
     8401 ttacgaagaa attaacaagc aggtcactaa caaaaaaatt gctctccaga ctctttttct
     8461 ttcgattact aatcaaaaag aggatgtcgc cttatatata ttatctaatt ttgagataac
     8521 tagacaagat gttatttcca taaagcatga gttgtatgat attgagtatc tacttagcgc
     8581 tcataattca agctgtaaag tacttgagta ttttatcaat aagggattgg ttgatgtaaa
     8641 cacaaagttc aaaaaaacta atagtgggga ttgtatgttg gataacgcaa taaaatatga
     8701 gaatgcagaa atgataaaac tattattgaa atatggtgca acatctgaca ataaatatat
     8761 ttaatcaaaa ttgaatatcg tttagcaaaa ggcagtattt gcacaaaata tcgatatatt
     8821 gtttattgtc agtatggctc aatgtgataa tagttggaaa gtttgatggg tttcgccccg
     8881 ttgtagcggt agtcgagctg gaaggtcttc aggcactgct tacagcgata gagcagcccc
     8941 cctgaactgg aatggccgtt ccggataccc cctgagtttc agagtaacgg ggacaaacca
     9001 catcaatctt tgccatcaat catccaaagg gcaaagagta caacaacact aagtctgcgt
     9061 cacaacccat caatgaaagg aatatataca tatgccatca gtaaatttaa tcccatcaag
     9121 gaaaatatgt ttgcaaaata tgataaataa agacaacgtc tctgttgaga caatccagtc
     9181 tctattgcac tcaaaacaat tgccatattt ttctgacaag aggagttttt tattaaatct
     9241 aaattgccaa gttaccgatc actctggaaa acttattgtc tgtcgacact tagcttccta
     9301 ctggatagca cagtttaaca aaagtagtgg tcacgtggat tatcatcact ttgcttttcc
     9361 ggatgaaatt aaaaattatg tttcagtgag tgaagaagaa aaggctatta atgtgcctgc
     9421 tattatttat tttgttgaaa acggttcatg gggagatatt attttttata ttttcaatga
     9481 aatgattttt cattccgaaa aaagcagagc actagaaata agtacatcaa atcacaatat
     9541 ggcattaggc ttgaagatta aagaaactaa aaatgggggg gattttgtca ttcagcttta
     9601 tgatcccaac catacagcaa ctcatttacg agcagagttt aacaaattta acttagctaa
     9661 aataaaaaaa ctgactgtag ataattttct tgatgaaaaa catcagaaat gttatggtct
     9721 tatatccgac ggtatgtcta tatttgtgga cagacatact ccaacaagca tgtcctccat
     9781 aatcagatgg cctaataatt tacttcaccc caaagttatt tatcacgcga tgcgtatggg
     9841 attgactgag ctaatccaaa aagtaacaag agtcgtacaa ctatctgacc tttcagacaa
     9901 tacgttagaa ttacttttgg cagccaaaaa tgacgatggt ttgtcaggat tgcttttagc
     9961 tttacaaaat gggcattcag atacaatctt agcatacgga gaactcctgg aaacttctgg
    10021 acttaacctt gataaaacgg tagaactact aactgcggaa ggaatgggag gacgaatatc
    10081 gggtttatcc caagcacttc aaaatgggca tgcagaaact atcaaaacat acggaaggct
    10141 tctcaagaag agagcaataa atatcgaata caataagctg aaaaatttgc tgaccgctta
    10201 ttattatgat gaagtacaca gacagatacc cggactaatg tttgctcttc aaaatggaca
    10261 tgcagatgct atacgcgcat acggtgagct cattcttagc ccccctctcc tcaactcaga
    10321 ggatattgta aatttgctgg cctcaaggag atatgacaat gttcccggac ttctgttagc
    10381 attgaataat ggacaggctg atgcaatctt agcttatggt gatatcttga atgaggcaaa
    10441 acttaacttg gataaaaaag cagagctgtt agaagcgaaa gattctaatg gtttatctgg
    10501 attgtttgta gccttgcata atggatgtgt agaaacaatt attgcttatg ggaaaatact
    10561 tcacactgca gaccttactc cacatcaggc atcaaaatta ctggcagcag aaggcccaaa
    10621 tggggtatct ggattaatta tagcttttca aaataggaat tttgaggcaa taaaaactta
    10681 tatgggaata ataaaaaatg aaaatattac acctgaagaa atagcagaac acttggacaa
    10741 aaaaaatgga agtgattttc tagaaattat gaagaatata aaaatctgaa tattatctcg
    10801 tggtgataca ctgaatttga ggtaaccggc tcatttaaac cgtctggtct gttacctccg
    10861 gttccacaaa aataatgtcc atcattttga agtggacact atcgtatgga ataccggacc
    10921 tggatcactg aagctttacg ccttcatttc gaagaacatt taccacgggt tgtggccgga
    10981 cgtcgcctgg gcgtaccaaa atcaactgtc tgtggtatgt tcgtgcgctt ccgcaacgct
    11041 ggcctttcat ggcctctgcc cgcaggtatg tcggagcagg aacttgatgc ccttctttac
    11101 ggaagtgcct ccacagtacc tgtcgtactt actgaaagca ccgttatgcc caaactcccg
    11161 gtggtaaaaa aacgtccccg gcggcc
//
LOCUS       DAJTNN010000134        11170 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTNN010000134 DAJTNN010000000
VERSION     DAJTNN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.134"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            217..873
                     /gene="pphA"
                     /locus_tag="OO150_003100"
     CDS             217..873
                     /gene="pphA"
                     /locus_tag="OO150_003100"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCR5517159.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(874..1065)
                     /locus_tag="OO150_003101"
     CDS             complement(874..1065)
                     /locus_tag="OO150_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5517160.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1170..1406)
                     /locus_tag="OO150_003102"
     CDS             complement(1170..1406)
                     /locus_tag="OO150_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5517161.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1524..2963)
                     /gene="rsmF"
                     /locus_tag="OO150_003103"
     CDS             complement(1524..2963)
                     /gene="rsmF"
                     /locus_tag="OO150_003103"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR5517162.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3043..5676)
                     /gene="yebT"
                     /locus_tag="OO150_003104"
     CDS             complement(3043..5676)
                     /gene="yebT"
                     /locus_tag="OO150_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR5517163.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLNNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVSGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5645..6928)
                     /gene="yebS"
                     /locus_tag="OO150_003105"
     CDS             complement(5645..6928)
                     /gene="yebS"
                     /locus_tag="OO150_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5517164.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7058..7555
                     /gene="msrC"
                     /locus_tag="OO150_003106"
     CDS             7058..7555
                     /gene="msrC"
                     /locus_tag="OO150_003106"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5517165.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7652..8350
                     /gene="proQ"
                     /locus_tag="OO150_003107"
     CDS             7652..8350
                     /gene="proQ"
                     /locus_tag="OO150_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5517166.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8370..10418
                     /gene="prc"
                     /locus_tag="OO150_003108"
     CDS             8370..10418
                     /gene="prc"
                     /locus_tag="OO150_003108"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5517167.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10610..>11170
                     /gene="htpX"
                     /locus_tag="OO150_003109"
     CDS             10610..>11170
                     /gene="htpX"
                     /locus_tag="OO150_003109"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5517168.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEE"
BASE COUNT         2852 a         2779 c         2888 g         2651 t
ORIGIN      
        1 aacgtaaatt tattcaacgc attaatttta aagggtttta ttgtttgttg acgaaaacag
       61 gaatcgtgtt cggtctcttt ttatctgtta aaagccagaa gcatttcctt cgctgacttt
      121 atagtcaacc ataacacaca ctctactgtc tgagtccagc gttttttaac attcttgtta
      181 agattatgtg atctttagca cgggaggaaa atattgatga aacagcctgc gcccgtttat
      241 cagagaattg cgggtcatca atggcgacat atctggcttt ctggcgatat acacggttgt
      301 cttgagcagt tgcgccgcaa attatggcat tgtcgttttg atccgtggcg agatttactt
      361 atctcagtgg gagacgttat cgatcgtggg ccgcaaagtt tacgttgtct gcagttactg
      421 gaacaacatt gggtttgtgc ggtaagaggc aatcatgaac agatggcgat ggatgcgctg
      481 gcatcccagc agatgtcttt gtggttgatg aatggcggcg actggtttat tgcgctggca
      541 gataatcaac agaaacaagc gaaaacggcg ctggaaaaat gtcagcattt gccctttatt
      601 cttgaagtac acagtcgcac cggcaagcat gttattgctc atgcggatta tccagatgat
      661 gtttatgaat ggcaaaagga cgttgatttg catcaggtct tgtggagccg ctcgcgatta
      721 ggtgaacgcc aaaaagggca gggaattaca ggtgctgatc atttctggtt tggtcataca
      781 ccgttgcgac atcgcgtgga tattggcaac ctgcattata ttgataccgg tgctgtcttt
      841 gggggcgaac tgactcttgt gcaattgcaa tgattaaaaa tcaccgtact cctgtgcagg
      901 tcgccagaaa ccatctataa aatcctcaat cggaaaacaa ccgccctggc ggatccgttg
      961 atcgctcata gaataaagac actgctgttc cgtgttgtag acatccacaa caatatcttc
     1021 acaaccgcca tccaggtagc aaacaaaaag taccagcgcg aacatttcat ccccgaagtg
     1081 tggtgccgta ccgttaagtt taggagagat tttacaacgg gggaataacc aggacaaata
     1141 acccgccatg ccgacgggtt ctttttggat caggcaagac gcataatcca ggcatcagat
     1201 tgttgatcgt aacgggtacg gtaaaggaca gagtgttcgc catttagcag ggcagggata
     1261 ctggtttcag catcccaggc atcgagttgc atacataaat cggggtcaga tttacaagga
     1321 atggttaacg ttcgatcacc cggcgattca ccgtgtaatt cgccgtcgtc gatttcaaaa
     1381 gcgcctatgc gcacactggt tttcatcttc gtgctctcca tttagctgcc ggttgtggta
     1441 cgaccagtcc gatcgcacca tttttcagcc tagttgaggc agccgaatgt gccagtcaaa
     1501 aagtgcgctt tttttgtgcg ctgtcaggcg ttaccggtaa aaagtttccc atcgcgcacc
     1561 agttcacgcg gatagctgtt tttcaatcgc gaaccaatcc gtttggctaa accaatcggt
     1621 tgatgctgga aagtaaccaa cacgtcatcc gccactggcg cggcttgcgg gtaaacatcg
     1681 cgcccgcgat accactcctc cgcttcctgc ggtgtcagtt caaaagcgtt cacattgtcg
     1741 ggggtggcaa gggcaataac tgcttcatgc tgccagcgat aacctttgtt gtgcgtttcg
     1801 gcaagtttaa tccccaagcg agaaaatcgg actttaccga tcagggcttc aatgcccacc
     1861 gggaacaacc acagttcttt gtcacgctgc cagaggcgca ggttttcatc ccagtttaag
     1921 ccaacacctg cagccgcctg gcgaatttgt ccagcttcgc gatctttcac cgggctgaac
     1981 ggaaaattac cgactttgta tttgggggcg ggtaaggcgg gaatcgcctg agttttacgc
     2041 agacgagcaa cgaagaagcc ttcgcagtcg taaatttgtg ggaaaacatg caaaaagcct
     2101 tcttcggtca gcgctttgtt tgcaccaggg aagagatcgc caagtggtaa aaactctact
     2161 gcgtcggggt aagtctcttt cagccacagg caaacggctt cgttttcttc ctggtttaag
     2221 gtacaggtcg agtaaaccag cgtaccacca ggacgtaatg catgaaaggc gctgtcgata
     2281 agctcccgtt gtgtagctgc gatttcctga ttgctttctg gtgaccagtt ttttagcgca
     2341 tcgggatctt tacgcaccac gccttcgccg gagcagggag cgtccagcaa aatggcatca
     2401 aacatttctg gcactgccgc accaaacacg cggccatcaa aatgtgtgag cgcaacatta
     2461 ctgatgccac agcggctgat attggcatgt aacactttta cccgactggc ggaaaactca
     2521 ttggcaagga ttgccccttc gttattcatc cgcgcggcaa tttgcgtcgt ttttgagcct
     2581 ggcgcagcag cgacatccat cacccgctgt ggtgcattac cgtcagcaaa caaggcggcg
     2641 acgggcaaca ttgaactggc ttcctgaata taaaacaggc cacttaaatg ctcggcggta
     2701 ctacccaatg gcaatgcatc ttcattgtcg cgttcaatcc agaaaccttc ttcacaccac
     2761 ggaattggcg taagcgtcca gccataagga gcggttaatt gcaggaaatc agcaacggag
     2821 attttcagcg tattaacgcg aatgctgcgg cgcaacgggc gctgacaggc ggcaagaaaa
     2881 tcatcaaatg agagcgtcga aggcatagct tcgcgcattt gtgtcagaaa cgcgtccggg
     2941 aaataaacgg tgtgttgggc cacgagcatg taccactggc aaaaaaacag gcgcgcagtt
     3001 tagcataaac gctccggcgc aggcacgccg gagcagtggg cattatttgg gaagcgcagt
     3061 tccccattca cgccactctt tcggttcact ttcctgcaac aggaagtgtt tgccttcctg
     3121 ggctttcggt gccagtggcg tacccggagg cgtggcgaag gcgatgccgc cacggataaa
     3181 ctggttaaag gtgccggttt tcactacgcc gcccgtcaga ccaaagtcca gactgtaacc
     3241 cgatgccaac cagaagacgg aattgttacg caccaggtgt tgatagcgtt tactgatgcg
     3301 catcgcaatc atcacgcgat ctgacaatgt ccccagcgtc attccggtaa ccgtacctac
     3361 ttccagacca cggaacagca caggcgtacc aatacctaac gaaccggctt ccggtgcttc
     3421 gacaataatg cttaagccat ccaggtaacg cgaatcagta atagtagctt cttgcaactc
     3481 aaagtcacgg cgaggattac cccgacctgg ttcgacattg atatacggct ggaggatagt
     3541 atctagatgc tcaacgcccg tggctgaaat ttgcggtgtg accaccgaga agcgcgtacc
     3601 gccgcgggcg aaggtctgga catactccgg atagagcacc gcctttgcct gtacttcatt
     3661 gcgcgcggta atcagatcca gcgtctggat ttgcccgata tcaataccga gatagcgaat
     3721 tggcatcccg accgccagtt ttccggcatc gaaagcgtga agcgtaatct gcccactaac
     3781 cgcacgggcc gctgtttcgg aagcatacag aattcgtttg tcgcctttac gctgactggc
     3841 gctggcaccg ctgaggttat cgaagctaat ggctcccttt aatgctctgg agagcgggga
     3901 tgcctgtacg gtcaggccac taccattcag ctgaactttc gccccgcctt ctgcccagaa
     3961 cacgctattg ctggtcagaa ggttgcgata ctccggctta atatgcagat cgatatcaaa
     4021 cgcgttagct cgcggacgca cggtaatcac ttcaccaact tcaaatttac ggtacagcac
     4081 taccgatcct gcctgcacat ccggcagcgt ctctgcactc aaactcacgg ttgtggtggg
     4141 taaatcgcta aggctgttct ccagcgcttt ttccagattg gcatacagtg gatagctggc
     4201 tttcatctca cctttatcgc ctggcagaat acgtatcccg ccgttaatcc attctgaggc
     4261 gctggcacca agaaactcaa cgccatccag ccccaccttc acgtcgacac ggctgttgac
     4321 gacaaatttg ctatcgcctt ttaccagttc gcgatgctga ggctcgatgg cgacggtaaa
     4381 ggtaacgcct ttgctggtga gtttacgatc aataacctgg cctacctgca cgccgtgaag
     4441 aatgagcggc tgacccgtat caataccgta actttccggc gcggtcaggg tcagcgtcag
     4501 aacatcaggt tcatgcagca gtgctttttc gcctggcaca acaacgaact ctttgcgtgg
     4561 ctcgccatcg ccgggtacca actcgaaggt tttgccagtc agcagggcgc tgagattggc
     4621 atcgctaagg gataatttcg gattacgtaa ttcgatgcgg gtattttcac gaagcagggt
     4681 aacgacgctg ggatcaactg tcatttcccc agtgacttta ccaccaggat ttaaatccag
     4741 tttagttagc tgtccgactt ccagtccctg atacattaat ggcgtcgagt cggcggttaa
     4801 tccggcccca ctcggcagtt ccagtttgat tattacgcca cgctggctat gggcgaggtc
     4861 ttcatacaga ccaaaggtat cttccgcctc ggcaggtttc gactcttctg gtgaatcgaa
     4921 ggcaatcgca ccgttaacca gtgccgccag gctttccagt ttcaccttcg cgccactgat
     4981 actgacattg gcatcaacgc cggaaacgtt ccagaaacgg ctaccttttt tcaccagatc
     5041 ggtaaaacgc cgctcgatca ggacatcaat caccacgcct tgtttgttgg gattgatggc
     5101 atagtcgtag acttttccca ccgggatctt gcggaaatag accaatgaac cgctgttcag
     5161 cgaaccgaga tcgggggctt gcaggtggat catcagatcg ccattgttca gccgatattt
     5221 cggttgggta tcgagtgcga caaagtgatc ctgctcttta cctgtacccg gcatcatgcc
     5281 gatatagttc ccaccgacga gggcgtccag cccggagaca cctgccaacg atgcttttgg
     5341 cgtcaccagc cagaattgag tctcttcgcg cagcgcatct ttcatatcgg acttgatgct
     5401 gaccttgact tcaatcttac gaagatcgtc gctgaggctg atatcctgca ctgttccgac
     5461 ttcaacgccc tgataacgaa caggcgtacg gcccgggaca ataccatccg ccgacataaa
     5521 gtcgatggtg acggtattac ttcggtcctg ataactgtcc caaatcagcc aaccggcaat
     5581 cattagcgcg atgaaaggca gcagccagaa aggtgagata cggcgtttat ttttaatctg
     5641 cgcttcagtc gtcgaagcgg gcgtttcctg actcatgtgc atcccaaagt aagcggctgt
     5701 ccagccattc cacagcaaga atagtcaaaa ttacggctgc gccaaaataa aacgcagccg
     5761 gtcccatagt aaaagcgagg atctgatcgc gattaatcag cgacatggtt aaagatatga
     5821 caaacaggtc gagcatcgac cagcgtccaa tccaggtcac catccgcagt aacagaatgc
     5881 gtgtgcgtaa accttgctgg catttaaaat gaatgctcaa cagtaaagtg aacatgacga
     5941 tcactttagt aaacggtacc agaatactgg cgataaacac gattccggcg accgcaatgt
     6001 tgctacttgc cagcgacata atcccggaaa gaatcgtatc ttcctgccgg ccaccattca
     6061 gataaatgat agaaataggc aataggttgg ctggtaacaa caaaacgatt gacgctaaca
     6121 gcgccgccca gcatttttgc aaactatgac gacgacgcag gcgtagcggg atatggcaac
     6181 gcgggcagcg accacgctga tcaggatagc cggtaaaatg gcacccaaga cagacacgaa
     6241 gtttctcgtc cctacgcgta gcggggcgct gcggataata tcgctcccac agctcctcaa
     6301 cattaagatg tgacaacgtc accgtcgtta aaatcaccaa cgccacaaaa gagaacaagc
     6361 cgacacccgc ctggatatgg gcataatcct gtacctttat agaagcaacg ccaatgccga
     6421 ccaggtagat atccagcatt acccactctt taagacgctc aagcatcagc agtaccggac
     6481 gtaaattcat tcccagtcgg ttaccaaacc ataaatatgc tatggaggac accagaatga
     6541 ggggggcacc aataacgcag aaaaagacca tcgcccccgt tatcgtatcg ccctgtttgg
     6601 tcatttgcca gatgccttgc atgacgtttg cgtcgatacg aatacctaac agccagatat
     6661 gcaacagcgg ttcgccccag gcaaacggca tcagcaacag catggtgaaa gccattgccg
     6721 ccaggcgcgt tagcgaccag tcgcgcccgt cgcgaatttt tgcctgacag cgcggacaat
     6781 aggcactctg atgagaattt atctcgggca ggctaaacag catgtcacat tgcgggcaac
     6841 gttggtaatc accacgcggc agttcctcgc cgattgacct cactgttatc ttttttgtcg
     6901 gcgtaatttg tggtgtgtta agagccatga cgtgccaaat gtaataagaa ttaacattaa
     6961 tcttaaccca tgatgggaat gatcttgagc attaacgcat ttaatgctta ttttaatagg
     7021 ctggcagttc caggtaagac aaacaagtga ttatataatg aacaaaacag aattttacgc
     7081 ggatttaaat cgcgacttta acgcgctgat ggcgggagaa accagttttc tggcaacgct
     7141 tgcgaacacc agtgcgttgt tatacgagcg tctcactgac gtaaactggg caggttttta
     7201 tttgcttgag gacgatacac tggtactcgg accatttcag ggcaaaattg cctgtgtccg
     7261 gatacccgtc gggcgcgggg tgtgcggcac tgcggttgcc cgcaatcaag tgcagcgtat
     7321 cgaggatgtt cacgcgtttg acgggcatat tgcctgtgat gcggcgagta attctgaaat
     7381 tgttctgccg ctggtggtga aaaatcagat tattggtgtt cttgacatcg atagtaccgt
     7441 cttcggtcgc tttacagacg aggacgagca aggcttacgt cagcttgtgg cacagcttga
     7501 aaaagtgctt gcaacgacgg attacaaaaa attctttgcg agcgtcgcag gataatcaac
     7561 ggataacgta gcatttactg gtagcgtcat tataatgacg cctgttcatg cctgcgcttg
     7621 ttggctacgt ccgttgtaat caggaaattt catggaaaat caacctaagt tgaatagcag
     7681 taaagaagta atcgcgtttc tggccgaacg ttttccccac tgtttcagtg cggaaggtga
     7741 agcgcgtccg ctgaaaatcg gtatttttca ggatttggtc gatcgtgttg ccggggaaat
     7801 gaacctgagc aaaacgcaat tacgatccgc tttacgtctc tacacttcga gctggcgtta
     7861 tctttacggt gttaaacccg gcgcaacgcg tgtcgatctt gacggcaacc catgcggtga
     7921 gctggacgag caacatgtag agcatgctcg caagcagctt gaagaagcga aagcgcgtgt
     7981 tcaggcacag cgtgctgaac agcaagcgaa aaaacgcgaa gctgccgcag ctgctggtga
     8041 gaaagaagac gcaccgcgcc gcgaacgcaa gccacgtccg accacgccac gccgcaaaga
     8101 aggcgctgaa cgtaaacctc gtgcgcaaaa gccggtagag aaagcgccaa aaacagtaaa
     8161 agcacctcgc gaagaacagc acaccccggt ttctgacatt tcagctctga ctgtcggaca
     8221 agctctgaag gtgaaagcgg gtcaaaacgc gatggatgcc accgtattag aaatcaccaa
     8281 agacggcgtc cgcgtccagc tgaattcggg tatgtctttg attgtgcgcg cagaacacct
     8341 ggtgttctga aacggaggcc gggccaggca tgaacatgtt ttttaggctt accgcgttag
     8401 ctggcctgct tgcaatagca ggccagacct tcgctgtaga agatatcacg cgtgctgatc
     8461 aaattccggt attaaaggaa gagacgcagc atgcgacggt aagtgagcgc gtaacgtcgc
     8521 gcttcacccg ttctcattat cgccagttcg acctcgatca ggcattttcg gccaaaatct
     8581 ttgaccgcta cctgaatctg ctcgattaca gccacaacgt gctgctggca agcgatgttg
     8641 aacagttcgc gaaaaagaaa accgagttag gcgatgaact gcgttcaggc aaactcgacg
     8701 ttttctacga tctctacaat ctggcgcaaa agcgccgttt tgagcgttac cagtacgctt
     8761 tgtcggtact ggaaaagccg atggatttca ccggcaacga cacttataac cttgaccgca
     8821 gcaaagcgcc ctggccgaaa aacgaggctg agttgaacgc gctgtgggac agtaaagtca
     8881 aattcgacga gttaagcctg aagctggcag gaaaaacgga taaagaaatt cgtgaaaccc
     8941 tgacacgccg ctacaaattt gccattcgtc gtctggcgca aaccaacagc gaagatgttt
     9001 tctcgctggc aatgacggcg tttgcgcgtg aaatcgaccc gcataccaac tatctttccc
     9061 cgcgtaatac cgaacagttc aacactgaaa tgagtttgtc gctggaaggt attggcgcag
     9121 tgctgcaaat ggatgatgac tacaccgtta tcaattcgat ggtggcaggt ggtccggcag
     9181 cgaagagtaa agctatcagc gttggtgaca aaattgtcgg tgttggtcaa acaggcaagc
     9241 cgatggttga cgtgattggc tggcgtcttg atgatgtggt tgccttaatt aaagggccga
     9301 agggcagtaa agttcgtctg gaaattttac ctgctggtaa agggaccaag acccgcactg
     9361 taacattgac ccgtgaacgt attcgtctcg aagaccgcgc ggttaaaatg tcggtgaaga
     9421 ccgtcggtaa agagaaagtc ggcgtgctgg atattccggg cttctatgtg ggtttgacag
     9481 acgatgtcaa agttcaactg cagaaactgg aaaaacagaa tgtcagcagt gtgatcatcg
     9541 acctgcgtag caatggcggt ggggcgttga ctgaagcggt atcgctctca ggtctgttta
     9601 ttcctgcggg tcccattgtt caggtccgcg ataacaacgg caaggttcgt gaagacagcg
     9661 ataccgacgg gcaggtgttc tataaaggcc cgctggtggt actggttgac cgtttcagtg
     9721 cttcggcttc agaaatcttt gccgcggcaa tgcaggatta cggtcgtgcg ctggttgtgg
     9781 gtgaaccgac gtttggtaaa ggcaccgttc agcaataccg ttcattgaac cgtatttacg
     9841 atcagatgtt acgtcctgaa tggccagcgc tgggttctgt gcagtacacg atccagaaat
     9901 tctatcgcgt taacggcggc agtacgcaac gtaaaggcgt aacgccagac atcatcatgc
     9961 cgacgggtaa tgaagaaacg gaaacgggtg agaaattcga agataacgcg ctgccgtggg
    10021 atagcattga tgccgcgact tatgtgaaat caggcgattt aacggccttt gggccggagc
    10081 tgctgaagga acataatgcg cgtatcgcga aagatcctga gttccagaac atcatgaagg
    10141 atatcgcgcg cttcaacgct atgaaggaca agcgcaatat cgtttctctg aactacgctg
    10201 tgcgtgagaa agagaataat gaagatgatg cgacgcgtct ggcgcgtttg aacgaacgct
    10261 ttaaacgcga aggtaaaccg gagttgaaga agctggatga tctaccgaaa gattaccaag
    10321 agccggatcc ttatctggat gagacggtga atatcgcact cgatctggcg aagcttgaaa
    10381 aagccagacc cgcggaacaa cccgctcccg tcaagtaata tcaatcaggc acaagaaatt
    10441 gtgcctgatt ttttaacagc ggcaagatgc cgtaaatcag atgctacaaa atgtaaagtt
    10501 gtgtctttct ggtgacttac gcactatcca gacttgaaaa tagtcgcgta acccatacga
    10561 tgtgggtatc gcatattgcg ttttgttaaa ctgaggtaaa aagaaaatta tgatgcgaat
    10621 cgcgctcttc ctgctaacga acctggccgt aatggtcgtt ttcgggctgg tactgagcct
    10681 gacagggata cagtcgagca gcgttcaggg gctgatgatc atggccttgc tgttcggttt
    10741 tggtggttcc ttcgtttcgc ttctgatgtc caaatggatg gcattacgat ctgttggcgg
    10801 ggaagtgatc gagcaaccgc gtaacgaaag ggaacgttgg ctggtcaata ctgtagcaac
    10861 ccaggctcgt caggcaggga tcgctatgcc gcaagtggct atctaccatg cgccggacat
    10921 caacgctttt gcaaccggtg cgcgccgtga tgcctctctg gttgctgtca gcaccggttt
    10981 gctgcagaac atgagcccgg atgaagccga ggcggtaatt gctcacgaaa tcagccacat
    11041 cgccaatggt gatatggtca ccatgacgct gattcagggc gtggtgaaca ccttcgttat
    11101 ctttatttcc cgtattctgg cgcagcttgc cgcgggtttt atgggcggaa atcgtgatga
    11161 aggtgaagag
//
LOCUS       DAJTNN010000135        11072 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTNN010000135 DAJTNN010000000
VERSION     DAJTNN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.135"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..734
                     /locus_tag="OO150_003110"
     CDS             <1..734
                     /locus_tag="OO150_003110"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5517169.1"
                     /translation="EIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTIVYNVN
                     DDTLDGNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGK
                     DLRASRAAAENIIPHTTGAAKAIGLEIPELSGKLKGHAQRVPVKTGSVTELVSILGKK
                     ATAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGDLQLVK
                     TVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(776..2216)
                     /gene="aldA"
                     /locus_tag="OO150_003111"
                     /pseudo
     CDS             complement(776..2216)
                     /gene="aldA"
                     /locus_tag="OO150_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2371..3213)
                     /locus_tag="OO150_003112"
     CDS             complement(2371..3213)
                     /locus_tag="OO150_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5517170.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLRQKLPVCEPLV
                     CKRA"
     gene            complement(3485..7387)
                     /gene="hrpA"
                     /locus_tag="OO150_003113"
     CDS             complement(3485..7387)
                     /gene="hrpA"
                     /locus_tag="OO150_003113"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5517171.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7588..8193
                     /gene="azoR"
                     /locus_tag="OO150_003114"
     CDS             7588..8193
                     /gene="azoR"
                     /locus_tag="OO150_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5517172.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8244..9560)
                     /locus_tag="OO150_003115"
     CDS             complement(8244..9560)
                     /locus_tag="OO150_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR5517173.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWPFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWPVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(9550..11072)
                     /locus_tag="OO150_003116"
                     /pseudo
     CDS             complement(9550..11072)
                     /locus_tag="OO150_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT         2745 a         3012 c         2645 g         2670 t
ORIGIN      
        1 cagaaatcat tgtcgaatgt actggctttt atacctccgc cgagaaatcg caggcgcatc
       61 ttgatgctgg tgcgaagaag gtgttgattt ccgcccctgc cggtgaaatg aaaactatcg
      121 tttataacgt caatgacgac actctggatg gcaacgacac cattgtttcc gtggcgtcat
      181 gcaccactaa ctgtcttgcg ccgatggcca aagccttgca tgacagtttc gggatagaag
      241 tcggcacgat gacgaccatt catgcctata ctggcaccca gtcactggtg gatggcccac
      301 gtggtaaaga tttacgtgct tcacgcgcag cggcagaaaa tatcattccc cacactacgg
      361 gggcggcaaa agccattggt ctggagatcc cggaactgag cggcaaactg aaaggtcatg
      421 cgcaacgcgt gccggtgaaa acaggttcgg tcactgaact ggtatcgatt ctcggaaaaa
      481 aagcgactgc cgaagaggtg aataacgcac ttaaacaagc aaccaccaat aacgagtcat
      541 ttggttatac cgatgaagaa atagtctctt ccgatatcat tggcagccat ttcggttcgg
      601 tgtttgatgc cacgcaaacg gaaattaccg ccgtgggcga tttacaactg gtgaaaacgg
      661 tcgcctggta cgataacgaa tatggcttcg tcacgcagct tattcgcacc ctcgaaaaat
      721 tcgctaaact ctgacgcgca caggcggagg aaaaaacctc cgcctctttt actcattacg
      781 actgtaaata aaccacctgg gtctgcagat attcatgcag gccatgttta ccatctgcgc
      841 cgccaatacc ggatttacgc catccggcgt ggaagccttg catagcttcg aagttttcac
      901 ggttgatgta agtttcacca aacttcagcc ctttaatggc tttcatcgcg acgttcagat
      961 tttgggtata gatttgatga ggtcaggccg taatcactgt cattagccat tgagatagcc
     1021 tcttccagcg tgtcaaatgc gaccaccggc agaaccgggc caaaggtttc ctcatgcata
     1081 atcgacattt cctggcgaac atccagcagc aatgtcggcg gataataata tcctttcccc
     1141 tctaccgctt tgccacccaa cgccactctc gccccttctt ctactgcgcg cgccactttt
     1201 tgctcaaccc tttccaacgc cgcggcgtta atcaacggcc ccatcgcaat gtcgttgcgt
     1261 tcagcggggt taccaaattg aaccgcctgc atcgcttcac ccagccgatt gacgaactga
     1321 tcataaatgc ctttctgtac ataaacacgt tctgcacagt tacacacttg cccactatta
     1381 atgacgcgtg aatcaacgat ggctttgact gccagttcaa gatcggcatc gtccattacg
     1441 atagctggtg ctttaccccc caattccaga cacactttgg tgatgttttt cgccgcagtc
     1501 gccatgatct tctcacctgc agagacgctg cctgtcatac tgaccattgc gacctttggg
     1561 ttacccgcca gttcttgccc aacggtttca ccacgcccca gtacaaggtt aaacacgccg
     1621 cgcggaaggc ctatttcatc gacgattttg gcgaatgcaa tcgcattgtt tggcgtaaat
     1681 tcactaggtt taatgacgat ggtattaccg gtcaaaagag cgggagccat tttgcgggca
     1741 atgaggaaga acgggaagtt ccacggcaga atgccggtag tcacaccaag cgcacgttta
     1801 aacaaaagaa tattttctcc tggacgatcg ctttgaataa tctcgccctc gtaacgccgt
     1861 gcccactccg ccatgtaatc gatatagtcg gcagtaaaag ccacttcgac ttcagccagc
     1921 tgctggatct tgcccccttc ttcaacaatc agcgcactga tttcactggc gcgttcgcgg
     1981 atcccggcgg agattttgcg caaccaactg gcgcgttcaa tagcaggcaa cgcttcccat
     2041 tctggttgtg cacgttctgc tgcatcgatt gccttacggg catcctcggc ctgaccatcg
     2101 ggtatgcggg aaatgacagc ctctgtagca gggtttacca catcaatcca tgcgtctcca
     2161 cgccaggtaa caaactgtcc atcgatatac ataggatgtt gaacgggtac tgacatgggc
     2221 gactcctgtg atttatatgt ttgttcttgg taaattcatt gttaattaac atcaatcatc
     2281 atagagacgg catcgtgaaa ggacaattct gtgaagggct tcataaaaac aagtgtaaaa
     2341 ggaataaaag gcagaaataa aagtcaggag tcatgcccgt ttgcaaacaa gtggttcaca
     2401 aacgggcaat ttttgacgta acgagcgact ttccatcgcc tcgatgagca ccgcatcgtg
     2461 tttaagcgtt tgccatgcat ggatgacttc tgccggaaaa tcaacgtgaa cgataaaatc
     2521 tcgcccgcgg ggaccgtagc catcgctatc atcgcgtaaa cgcggcagct cgccagtgag
     2581 tagtgagaga taacgctcaa ccggccataa tccttgtaac tgattaataa agattacact
     2641 gtctgcgtta tttcctaact gaggaacgaa tccgggataa cttaaccagc gtggtgcatc
     2701 gggattgtcc ccagtcatac ggcggaacgt cgccatcgtg cgccgctgca tggtggggtc
     2761 ctgaacaacg atagccgtat gaactcgttc tacggcctga ttaaatagcg cgatgctaaa
     2821 gcgtgcgttt tcaccgcagt ttgttgactg gtcttcaatc cagatttttt catgcggaat
     2881 gtgccagaac tgatgagcga tatccgccag gatggtcgct tctgctctgc cagtggtgcg
     2941 gatagtgttg tagtgcggat gctgtgcgat ggcgctatac aaaaaagttg tcgagtgacc
     3001 gataccacca ctaatcagta aaggaatttg ctgatcgcga gcaatcttac atgccgcgtc
     3061 gatagtcggc ataaccgcat tgcctgcaag gatcacgcaa tcggcctgat acggcacctc
     3121 accggagaaa tcatcctgcg ccagccactg tccgataaca tttatggcat ctatagttgc
     3181 cggcgaaagc gtcggaaacg gggtaatgtt catggttcct ccttatcgtt cccttaagaa
     3241 tactgcgtga gataatagtg agaagctgag cactctgaaa ggaggaagtc gtatgtcctt
     3301 aaccgccact ccgcaagcct gacatatctt ttaaggaaat gctgtagata aacattattc
     3361 atcttgcagc agagaattgt atttttctga attcctgaat ttttaccctt accgttttag
     3421 aattaataaa aaacccggcg ctaaatgctt accgggtttt agatttatta ggcaaatagc
     3481 agggttaacc gctaatctgc tccatcgcct gcaaaatacg cttatctgaa atcggataag
     3541 gcgtaccaag ttgttgagcg aagtaactaa cgcgcagctc ttctatcatc caacggatct
     3601 ctttcacgtc ttcatcctca cgacgtgcag gcggcagttt gttgatccat tgctgccacg
     3661 cctgctggac gttttcgact ttcagcatct gtgcacgatc gcgatgtgga tcaaccgcca
     3721 gtttttccag tcgtttttca atcgcctgca aatatcgcag cgtgtcgccc agccgtttga
     3781 agccgttacc agtgacaaaa ccgcgatata ccaacccgcc catctgcgct ttaatgtcag
     3841 aaagccccag tgccatggtc atatccaccc gccctttcag acgtttgttg atattgaaca
     3901 ctgccgtaag aatttgctcg acctgcttcg caatatctac caccgtgtcg ttcagttcgg
     3961 cacgcacttt ctcatgcagc gcagcaaagc cttcttccgt ccagaccggg ccaccattgg
     4021 cgtcgatcag ttgatccaca ccgcaggaga tacagtcgtc gatcagctcc agcactttgc
     4081 catacgggtt gaagtacagt cccagcttgg ctttgttcgg taacttttca tgtaaatact
     4141 tgatcggcga gggaatattt agcagcagta gacggcgaag accattccac attgcctgtt
     4201 tttgctccag cgggttatca aacagtttga tcgccacgct gtcgcgttca tccaccagcg
     4261 ccggccacgc cttcacttta tagttgccac gcttctgttc gtaactttcc ggcagctgac
     4321 caaaactcca gatatgtaag ccgctctgct cgataccgtc atccgccacc gcggacagcg
     4381 tttcctgcac tttgcctttc agcgcatctt tcagatcttg tagcgagcgc ccttctttta
     4441 gcttcttgtt tttgtcatcc accacgcgga aggtgatttt caggtgatcg ggcacctgat
     4501 cccagtgcca gtcttcgcgg tcaacggtaa cgccggtcat ccgccgtaac tcgcgctcaa
     4561 ggctgtcgag caacggtaac tccagcggtg tgacgcggcc taaaaacgct tcggcgtagt
     4621 ttggtgcagg cacaaaatta cggcgtaccg gtttcggcaa cgatttaatc agagcaatca
     4681 ccaattcgcg gcgcagaccg gggatctgcc attcaaaccc gctttcctca acctggttaa
     4741 gtaacggcag cggaatatgt acggtcacac cgtccgcgtc tgcgcctggc tcaaactgat
     4801 agctcaaacg cagcttgaga ttgccctgat gccagaagtt cgggtaatcc agcttgctga
     4861 ttttttccgc cccttctttg atcaacatgc tcttttcaaa gttgagcaaa tcaggcgttt
     4921 cgcggctgac ttttttccac cagctatcga agtgacgagc ggagattaca tcgtggctga
     4981 tgcgctggtc gtagaactca aacagcgttt cgtcatcaac cagaatatcg cggcgacgtg
     5041 atttgtgttc cagctcttcc acttcggccc gcagtttcag gttttcacgg aagaatgcgt
     5101 gacgcgtctg ccagtcacct tccaccagcg catggcgaat aaagagttca cgacataacg
     5161 ccggatcgat ctggctgtag ttgaccttgc gcgcggcaac aatcggcaaa ccataaacag
     5221 tgactttttc cgttgccatc accgcgccct gcgcccgttc ccagtgcggt tcgctgtagg
     5281 tacgtttaat caaatgctga gcgacaggct ccacccattc cgggtcgata cgcgcagcaa
     5341 tgcgccccca caggcggctg gtttctacca gttccgccac cattacccat ttcggcggtt
     5401 ttttgaataa accagaaccg gggaagatgg agaaacgcgc gttacgtgcg ccggtatatt
     5461 cttgtttatc ggcatctttc atgccgatat gggaaagtaa acccgtcagc aacgcaatgt
     5521 gaatttcgcg atactccgcc ggttcgctgt taaccggaat gccaagttct ttcaccacct
     5581 gacgcaactg ggtgtagata tcctgccatt cgcgcacgcg cagatagttg agataatcgg
     5641 tacgacacag gcgacggaag gcgtttgaag aaagcgcctt ttgctgctcg ccaagataat
     5701 tccacagatt tacaaacgcg aggaagtcgg attctttgtc gtggaagcga cgatgttttt
     5761 cgtccgatgc ctgctgtttg tccatcggcc gctcacgcgg gtcctgaatg gagagcgcgg
     5821 acgtgataat catcgcctca cgcacgcagc catgtttctg cgcttccagc accatacgcg
     5881 ccagacgagg atcgaccggc agttgcgaga gctggcgacc gagcggcgtc agtttatagg
     5941 cgctggcctg ttcatccgtg gtgatcgcgc ccagttcttc gagcagacgc acgccatcct
     6001 ggatattgcg tttatccggt gcttcgacaa acgggaacgc agcgatatcg cccagcccca
     6061 gcgcggtcat ctgcaaaata accgaggcca ggttggtacg cagaatctcc ggatcggtaa
     6121 actccgggcg cgagaggaaa tcgtcttcgg aatagagacg aatacagatc ccttcggaca
     6181 cacgaccaca acggccttta cgctgattgg cagacgcctg tgaaatgggt tcaatcggca
     6241 aacgctgcac tttggtgcga tagctgtagc ggctgatacg cgccgtaccg gggtcgataa
     6301 cgtatttaat ccccggtaca gtcagcgagg tttccgcgac gttggtcgcc agcacaatgc
     6361 gtcgcccgct gtgcgactgg aacacgcggt tctgttcgct attcgaaagc cgcgcataaa
     6421 gcggcaagat ttcggtatgg cgtaagttca gcttgttcag cgcatcggcg gtatcgcgaa
     6481 tttcccgctc gccgctcata aagatcagaa tgtcgccagg gctttcctgg ctcagttcgt
     6541 ctacggcgtc aaaaatcgcc tgcaactgat cgcgctcggt gtcatcggct tcttcaacaa
     6601 tcgggcgata gcgcacttcc accggatagg tccgaccgga gacttcgata atcggcgcat
     6661 tattaaagtg gcgagaaaag cgttccgggt cgatagtcgc ggaagtgata atgattttta
     6721 ggtcaggacg ccgcggcagc aactctttca aatagccgag cagaaaatcg atattcaggc
     6781 tgcgttcgtg tgcttcgtca ataatgatgg tgtcgtactg catcagcagg cggtcttgct
     6841 ggatctccgc cagcaggata ccgtcggtca tcagcttgac catcgtgtta tcacttacgt
     6901 gatcgctgaa acgcactttg taaccgatgc aaccgcctgg ctccgttttc agctcttccg
     6961 caatacggtt cgccaccgtt cttgccgcca gacgacgcgg ctgggtatgg ccgatcagtc
     7021 ctttaatccc gcgccccagc tccatacaga ttttcggtag ctgggtcgtt ttaccggaac
     7081 cggtttcccc ggcgacgatc accacctggt gatcacgaat cgcttcgaga atatcctgtt
     7141 ttttctgact aactggcaaa ttgtccggat aagtgatttc cggtcgtgcc gcttcacgca
     7201 gcaggacttt ccctgccgcc tggtcaatct ctttcgccat ctcctggaaa atggcctgtt
     7261 gtgcatcagg atttttaacc ttcttaacac catgcagacg gcgagaaaaa cgcagtctgt
     7321 cgcgcagcat caacgaatcc agccgctgct gcaaagcagt aaaggtcaat ttttgttgtt
     7381 ctgtcataac gttagtgggc agtagtctgc ctgattaatt ttcttttaaa tgatagatat
     7441 agagtaccac atcgcggctt tctatgcgtt gttcaataaa ttcgaacata ggcttcgatt
     7501 tattgcgcta tctctgtggc aggattgtga ataaagtgtc agcaagcaac ggggcatcgc
     7561 caattcaaac atctataagg aaacaccatg agcaaggtat tagttcttaa atccagcatc
     7621 ctggcagggt actctcagtc taatcagttg tccgattatt ttgttgaaca atggcgcgaa
     7681 aagcactccg ctgatgaaat caccgttcgc gacctggctg caaatccgat tccggtactg
     7741 gatggcgaac tggttggcgc tctgcgtccg agcgatgcgc cgctgactcc gcgtcagcag
     7801 gaagctctgg cgctttccga tgagctgatt gccgagctga aagcccacga cgttatcgtt
     7861 attgcggcac cgatgtataa cttcaacatc tcaactcagt tgaaaaacta ttttgacctg
     7921 gttgcacgcg caggcgttac tttccgctat accgagaacg gtccggaagg tctggtaacg
     7981 ggtaaaaaag ccatcgttat taccagccgc ggtgggatcc acaaagatgg accaacggac
     8041 ctggtgacgc cgtatctgtc cacgttcctc ggctttatcg gcattaccga tgtgaaattt
     8101 gtcttcgccg aagggatcgc atacggtccg gaaatggcag cgaaagcgca gtctgacgcg
     8161 aaagcggcca tcgacagcat tgttgctgca taaccggaca cgatcttgat gtggactgta
     8221 gttcagtcca catctcaatc cacttacctg ttttcccata atttcagcat cgctttgtga
     8281 tcttccttaa gcacaatatg cgaacgtctg gctcgaatat aactaatcgc ttcatcaacg
     8341 gttttacagt gtccgtaaca taacaaccat gccgccacta ccagcgcact gcgcgataat
     8401 cccaacgcgc aatggaccag aacgctgcct tgctcttcgc gtaatgtttc cagcatcgcc
     8461 acggcctgtc ggagctcccc ctcttccgga accaccagat ccagcatcgg tacacagaaa
     8521 tagagtcgat cttttgtcgc tcgtccccga gggaattcaa aggtgacgtc cagaaccgca
     8581 ttctgtgccg gaatatgtcg tggaaacgcc cctaaataaa caccagcagt aattttgctc
     8641 accggctcca ggcgacgaca aaaccaacgc atagacagcc acatcccgat gcgccagggc
     8701 aatgtcagcc agtaaacggc gggcgggagt ttcccctgac tatctttccc tgttgttatc
     8761 gcgccaagcc caccgtaccc acggccaata atgagtagcg ataatactgg ccaacataac
     8821 cagactggcc accataactg aatcatcacc attagctcca tcaacacaat gcacgagccc
     8881 gcgcctacga catatggcag tgctattttg attcgacgtt gatcaggttt ctgataattc
     8941 caacgacgat cgacgggcac catccagtca atcaacatac ctaccgccag ccctgtgatg
     9001 acatcaataa aatgatgctg ccaggtcgtc agagtagaaa tagcgatgag taaaaaccat
     9061 ccgccacaga ctttacgcca cctctcagcc agatgctgac gaaagtgacg ccaaagtagc
     9121 cagcagagaa taatatgcag cgaaggagac tggttataag gcagatcaaa cagttcaagt
     9181 tgcgaaaatg gccatcccgt caccccactc acttcaggac ggataaaact aaacttcagc
     9241 ggatagagca aaaaaccgca gcaggccatt accgttgcca gaataagccg gtggacaagt
     9301 cggcgctgtt cgaatgtcgt gctacaaacg aacagcgaaa atccatataa aagatccaga
     9361 ctccagtaag gaacaatagt ccagggaaga aaagggatcg ccgtttccca accgaatacc
     9421 tgactgggga tatcatggct gttaaggtcc tgaaccgcgg tgaactgatt aagagatcca
     9481 taggtgaaga aaaaaaacgg ggccagcaac aataaccagc cagcgccttg tagcaatacg
     9541 ttccgacgtt cagttatcac gacgcaccgc catcgaaacc gtaaaaatac cccactcatc
     9601 aatccgttgt gtgcatttat caaatccggc atcacgcacg agtgaatcca tctccccttg
     9661 cgaacgcacg cgcattaccc acggtttacc atctttatga ctggttaaca ccccggcaat
     9721 catctccagt tgagggtgcc acggctgccc ggtgtagatg agaatgccga ccggttcgat
     9781 ggcattggca agacctgcga gtgagttttt tacctgctca ttttcgggaa aaagctcata
     9841 cagtccagag acaattgcca gcgtaggccg cggagttaac gcgctgagtt cctccgggtt
     9901 aaacgcatcg ccctgttcaa aacgcacccg cccagacatt ccccgttggg caatcatctc
     9961 ttgcccctgt gcaacattta actcgctgta atcacgtaac aaaatatcgc ttacggcagg
    10021 ctcgtttgcc agcgcatcca gtacatagcg cccatgccct gcggcaatgt caaccacgcg
    10081 gacggctaaa cctttggcgt ggagatcggc aacggcttgt ttaatcagta tttgtaaatg
    10141 ggttttgcgc tggcgaatac cgcgccagcc aacactgttc aggtagattt tgtcgattaa
    10201 gcgcccgaat gcgttactac cttgcggttg attttgatag acataatcca gcgaactgcc
    10261 tgaatcaaag ccggtgctca ttccgagatg caggcccgca gagtgcgtcc cgaacaattt
    10321 catcgccttt cgcataaagc gatacgctaa atcaaccggc gataatagca cgggtccacc
    10381 agaaagtagc cgccattgat ccgctgatgg tcctgtgcag tcttcatgct gataatcaaa
    10441 tttttgcgat ttgttagcat ataaacgact aataaagctt tgcatttttt caaatgccag
    10501 cgccctgttc tcttcaccca acgtgtcgtg ataaaagcct ggcagcagat gcagctcttt
    10561 cagagggcta cgtaatctct gataaaaatc aatctgcggt tggcgatgca ccacatagtc
    10621 atcgcctgat atcagaagtt gcgtggggag cgtaatcgcc gccgcatctc taataatacg
    10681 ttcagacgtt ttatagagat cgagcaagat attgacggca atcgcccgtg tgatcagcgg
    10741 atcattattg aaactcgcca cccgttgccg atcgtgggtc aaatagcgtc ctttcacata
    10801 ggaattaata aaaaacagac cacgcagacg atgccataac gccagcgcag gacgtgccag
    10861 cggcacatac aatttaacct taaaggccgg agaagccagc accagcccgc gaattgcagg
    10921 cgcataatca tgaacctatg tggcaaccag cactgcgccg acgctttgcg cgatcacaac
    10981 cacctcttcc agtccaacct agctgtcgct ggcagcaaaa cggacaaatt catcgacatc
    11041 ctgcactgaa cgcgcaagag atgggctata ac
//
LOCUS       DAJTNN010000136        10973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTNN010000136 DAJTNN010000000
VERSION     DAJTNN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.136"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..474
                     /locus_tag="OO150_003117"
     CDS             <1..474
                     /locus_tag="OO150_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5517174.1"
                     /translation="GGPKPFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVIL
                     TITSMAAENKNINMTSYASSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSV
                     ITPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            703..1293
                     /gene="uidR"
                     /locus_tag="OO150_003118"
     CDS             703..1293
                     /gene="uidR"
                     /locus_tag="OO150_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5517175.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1681..3492
                     /gene="uidA"
                     /locus_tag="OO150_003119"
     CDS             1681..3492
                     /gene="uidA"
                     /locus_tag="OO150_003119"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5517176.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLEWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3489..4862
                     /gene="uidB"
                     /locus_tag="OO150_003120"
     CDS             3489..4862
                     /gene="uidB"
                     /locus_tag="OO150_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR5517177.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            4901..6166
                     /gene="uidC"
                     /locus_tag="OO150_003121"
     CDS             4901..6166
                     /gene="uidC"
                     /locus_tag="OO150_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5517178.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQRGLFDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(6392..7900)
                     /locus_tag="OO150_003122"
     CDS             complement(6392..7900)
                     /locus_tag="OO150_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5517179.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8001..9176)
                     /gene="manA"
                     /locus_tag="OO150_003123"
     CDS             complement(8001..9176)
                     /gene="manA"
                     /locus_tag="OO150_003123"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5517180.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9375..>10973
                     /gene="fumB"
                     /locus_tag="OO150_003124"
     CDS             9375..>10973
                     /gene="fumB"
                     /locus_tag="OO150_003124"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5517181.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGND"
BASE COUNT         2735 a         2565 c         2833 g         2840 t
ORIGIN      
        1 ggtggaccta aaccgtttga tatgccaatg gcggattttc gccgtgctta tgaactgaat
       61 gtgttttctt ttttccatct gtcacaactt gttgcgccag aaatggaaaa aaatggcggt
      121 ggcgttattc tgaccatcac ttctatggcg gcagaaaata aaaatataaa catgacttcc
      181 tatgcatcat ctaaagctgc ggccagtcat ctggtcagaa atatggcatt tgacctgggt
      241 gaaaaaaata ttcgggtgaa tggcattgcg ccgggggcaa tattaaccga tgccctgaaa
      301 tccgttatta caccagaaat tgaacaaaaa atgttacagc acacgccgat cagacgtctg
      361 ggtcaaccgc aagatattgc taacgcggcg ctgttccttt gctcgcctgc agccagctgg
      421 gtaagcggac aaattctcac cgtctccggt ggtggggtac aggagctcaa ttaatacact
      481 aacgaaccgg taaacagccg tatgtgttgt ttaccgggat agcgtgtgca attcatcaac
      541 gtctctgcta aacaactggc agccaaatca cggctattgg ttaaccaata gctggatgaa
      601 aagcatacga atagagtgtg ccttcgcact attcaacagc aatgataggc gctcacctga
      661 caacgcggta aactagttat tcacgctaac tataatggtt taatgatgga taacatgcag
      721 actgaagcac aaccgacacg gacccggatc ctcaatgctg ccagagagat tttttcagaa
      781 aatggatttc acagtgcctc gatgaaagcc atctgtaaat cctgcgccat tagtcccggg
      841 acgctttatc accatttcat ctccaaagaa gccttgattc aggcgattat cttacaggac
      901 caggagaggg cgctggcccg tttccgggaa ccgattgaag ggattcattt tgttgactat
      961 atggtcgagt ctattgtctc tctcacccat gaagcctttg ggcaacgggc gctggtggtc
     1021 gaaattatgg cggaagggat gcgtaaccca caggtcgccg ccatgcttaa aaataagcat
     1081 atgacgatca ctgaatttgt tgcccagcgg atgcgtgatg cccagcaaaa aggcgagata
     1141 agcccagaca tcaacacgtc aatgacttca cgtttactgc tggatctgac ctacggtgta
     1201 ctggccgata tcgaagcgga agatctggcg cgtgaagcgt cgtttgctca gggattacgc
     1261 gcgatgattg gcggtatctt aaccgcatcc tgatcctctc atttcgcggg ctggtgataa
     1321 ctgtgcccgc gtttcatact ttaatttccc tgtacaaaat acaatctagc cgggatcgga
     1381 gaattccccc ccaaaatatt caccgtagcc atatgtcatg agagtttatc gttctcaaga
     1441 tgctcgaacg aacgttcgat tgcttatttt atggcttctg tcaacgctgt tttaaagatt
     1501 aatgcgatct atatcacgct gtgcgtattg cagattttgg ttttttgatc gcggtgtcag
     1561 ttctttttat ttccatttct cttccatggg tttctcacag ataactgtgt gcaacacaga
     1621 attggttaac taatcagatt aaaggttgac cagtattttt atttgaatga ggagtccctt
     1681 atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca
     1741 ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa
     1801 gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt
     1861 cgtaattatg tgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca
     1921 ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat
     1981 aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg
     2041 tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg
     2101 cagactatcc cgccgggaat ggtgattacc gatgaaaacg gcaagaaaaa gcagtcttac
     2161 ttccatgatt tctttaacta tgccgggatc catcgcagcg taatggtcta caccacgccg
     2221 aacacctggg tggacgatat caccgtggtg acgcatgttg cgcaagactg taaccacgcg
     2281 tctgttgact ggcaggtggt agcaaatggt gatgtcagcg ttgaactgcg tgatgcggat
     2341 cagcaggtgg ttgcaactgg acaaggtacc agcgggactt tgcaagtggt gaatccccac
     2401 ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca
     2461 gagtgtgata tctacccgct gcgcgtcggc atccggtcag tggcagtgaa ggacgaacag
     2521 ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggat
     2581 ttgcgaggca aaggattcga taacgtgctg atggtgcacg atcacgcatt aatggactgg
     2641 attggggcca actcctaccg aacctcgcat tacccttacg ctgaagagat gctcgagtgg
     2701 gcagatgaac atggcatcgt ggtgattgat gaaactgcag ctgtcggctt taacctctct
     2761 ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggctgtc
     2821 aacggggaaa ctcagcaggc gcacttacag gcgattaaag agctgatagc gcgtgacaaa
     2881 aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt
     2941 gcacgagaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg
     3001 atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt
     3061 gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg
     3121 gcagagaagg tactggaaaa agaacttcta gcctggcagg agaaactgca tcagccgatt
     3181 atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg
     3241 tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc
     3301 agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata
     3361 ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg accgcaaacc gaagtcggcg
     3421 gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga
     3481 ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt cggctacagc ctcggtgacg
     3541 tcgccaataa cttcgccttc gcaatggggg cgctcttcct gttgagttac tacaccgacg
     3601 tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt attggtgcgg gtattcgatg
     3661 ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt gaatacccgc tggggaaaat
     3721 tccgcccgtt tttactcttc ggtactgcgc cgttaatgat cttcagcgtg ctggtattct
     3781 gggtaccgac cgactggagc catagcagca aagtagtgta tgcatatttg acctacatgg
     3841 gcctcgggct ttgctatagc ctggtgaata ttccttatgg ttcacttgct accgcgatga
     3901 cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg tgggattgcc gcttcattga
     3961 cctttgtctg cctggcattt ctgatagggc cgagcattaa gaactccagc ccggaagaga
     4021 tggtgtcggt ataccatttc tggacgattg tgctggcgat tgccggaatg gtgctttact
     4081 tcatctgctt caaatcgacg cgtgagaatg tggtacgtat cgttgcgcag ccgtcattga
     4141 agatcagtct gcaaaccctg aaacggaatc gcccgctgtt tatgttgtgc atcggtgcgc
     4201 tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc gttgttctac gagcgctatg
     4261 tgttaaatga taccgggctg ttcactgtgc tggtactggt gcaaaacttg gttggtactg
     4321 tggcatcggc accgctggtg ccggggatgg tcgcgaggag cggtaaaaag aataccttcc
     4381 tgattggcgc tttgctggga acctgcggtt atctgctatt cttctgggtt tccgtctggt
     4441 cactgccggt ggcgttggtt gcgttggcca tcgcttcaat tggtcagggc gttactatga
     4501 ctgtgatgtg ggcactggaa gctgataccg tagaatacgg tgaatacctg accggcgtgc
     4561 gaattgaagg gctcacctat tcactattct catttacccg taaatgcggt caggcaatcg
     4621 gaggttcaat tcctgccttt attttgggat taagcggata tatcgccaat caggtgcaaa
     4681 cgccggaagt tattatgggc atccgcacat caattgcctt agtaccttgc ggatttatgc
     4741 tactggcatt cgttattatc tggttttatc cgctcacgga taaaaaattc aaagaaatcg
     4801 tggttgaaat tgataatcgt aaaaaagtgc agcagcaatt aatcagcgat atcactaatt
     4861 aatattcaat aaaaataatc agaacatcaa aggtgcaact atgagaaaaa tagtggccat
     4921 ggccgttatt tgcctgacgg ctgcctctgg ccttacctct gcttatgcgg cgcaactggc
     4981 tgacgatgaa gcgggactac gcatcagact gaaaaacgaa ttgcgcaggg cggataagcc
     5041 cagtgctggc gcgggaagag atatttacgc atgggtacag agaggattgt tcgatttcaa
     5101 tagtggttat tattccaata ttattggcgt tgaaggcggg gcatattatg tttataaatt
     5161 aggtgctcgt gctgatatga gtacccggtg gtatcttgat ggtgataaaa gttttggctt
     5221 tgccctgggg gcagtaaaaa taaaaccctg tgaaaatagc ctgcttaaat taggtcgctt
     5281 cgggacggat tatagttatg gtagcttacc ttatcgtatt ccgttaatgg ctggcagttc
     5341 gcaacgtaca ttaccgacag tttctgaagg agcattaggt tattgggctt taacaccaaa
     5401 tattgatctg tggggaatgt ggcgttcacg agtattttta tggactgatt caacaaccgg
     5461 tattcgtgat gaaggggtgt ataacagcca gacgggaaaa tacgataaac atcgcgcacg
     5521 ttctttttta gccgccagtt ggcatgatga taccagtcgc tattctctgg gggcatcggt
     5581 acagaaagat gtttccaatc agatacaaag tattctcgag aaaagcatac cgctcgaccc
     5641 gaattatacg ttgaaagggg aattgctcgg cttttacgcg cagctcgaag gtttaagtcg
     5701 taataccagc cagcccaatg aaacggcgtt ggttagtgga caattgacct ggaatgcgcc
     5761 gtggggaagc gtatttggca gtggtggtta tttgcggcat gcaatgaatg gtgccgtggt
     5821 ggataccgac attggctatc ccttttcatt aagtcttgat cgtaaccgtg aaggaatgca
     5881 gtcctggcaa ttgggcgtca actatcgttt aacgccgcaa tttacactga catttgcacc
     5941 gattgtgact cgcggctatg aatccagtaa acgagatgtg cggattgaag gcacgggtat
     6001 cttaggtggt atgaactatc gggtcagcga agggccgtta caagggatga atttctttct
     6061 tgctgccgat aaagggcggg aaaagcgcga tggcagtacg ctgggcgatc gcctgaatta
     6121 ctgggatgtg aaaatgagta ttcagtatga ctttatgctg aagtaagaaa taacgccgga
     6181 gagaaaaacc tccggcgttt cagattgttg acaaagtgcg cgttttttat gccggatgcg
     6241 gcgtaaacgc cttatccagc ctacaaaaac tcataaattc aaagtattgc aggaaaaggt
     6301 aggcctgata agcgtagcgc atcaggcaat ctctggtttg ttttcagatg aaaacgccga
     6361 agtgaaaatt ctccggcgtt ttggccgtga attactgctg cggaattgcc ggtacagccg
     6421 gaacgttaag agctggcatc gcaaacatgc caacaaaatc ttctaacgac attttctgcc
     6481 catttaacgt tatctgaccg ttagtatatt gcaggctggt ggtgatggta ttgtcctgca
     6541 aggtggtcag acggaacatc tgccccattg ctgatgcacc ttcaacttgc tgtttcgcca
     6601 gttttttcgc ttgatcttcc tgataacctt ccagcttcgc tacctgagtc ataaactcag
     6661 ttgccatatc caccggaatg gtcagtttcg catccagaga tttaaccgaa cgatctactt
     6721 cctgcgccag cgtttgcggc gcttctttag tcgttgccgg atctttcagg aacagcgaca
     6781 gattcagcgc actttcaccc tgactgtttt tccagcttag cggcgcgaca gtaatcaccg
     6841 gatcgccttt caacatcagc ggcagggcgc taaagaaggc ttccgtcact ttctcctgat
     6901 aaagttcggg attgttggca atttctggct gtgccagcag cgcctgagtt tgcgcgttat
     6961 attgctggct gaactgatgc caggcttcac catcaatctg gccgactttt aaagtcagct
     7021 tgccgctgcc cagatcctga ttctgtacct tcaggctgtt tagcgagtaa tccagttggc
     7081 tattgatcgt tttaccgtca ttgaccagat ccgatttacc gctgatctcc atgccttcca
     7141 gcagtgccag ttctttgcct tccactgaaa tggtcatttt ttccagtgac agtttttggt
     7201 ttcctacgcg ctcaccaaaa cttgccagcg tgctggaacc gtcggttttc agattattaa
     7261 aggtcaactg cactttctgg ttgtattcgt taactgcgtc tatccgacca ctttgcgcct
     7321 ccccggaaag ggagatggct ttgccgtctc tgtcagcatt taactggaac tcgccgccgc
     7381 taaaggcgac tttttcatcc ttttgctcgt aattcagtgg cttgagcgaa atatcggaac
     7441 tggaatcacc gctgtaacca atgcgcgagt taatctcaaa aggcgtttca ccttttgcca
     7501 tatcaaacag tggtttgctt acttcgttat taaccagcgt ggtttgaatt gatgccatcg
     7561 acgggatcag gttcagtttt ttaagctggg caagcgggaa gggaccatga tcaaccgatt
     7621 cgttgaagat gacgctctga ccgcttttaa tccacggatt ttctttcccg gcaatgggtt
     7681 tcaccaacag ttgcaactgg ctgctgaata cgccgcgatg atagttttga taactcactt
     7741 ccaggttgga ttcaggagct gtcagtttta gttgcgcgtt cgcctgcgcg accatgtctt
     7801 cgagatgggt ttcaatcttc ttgcctgtat accatgcgcc gcctgtccag actacgccta
     7861 gcgcaacaat gacgcctacc gctaccagcg atttattcat aatgattatc cataaaatga
     7921 aatcaggcgg actggccgcc tgaaggtgtt ataagccttt aataagctta gcaagagatg
     7981 ttaatttttt cagtaagctc ttacagcttg ttgtaaacac gcgctaaacg gccgtggcct
     8041 ttgacagtca ccggtgattc gttggcggca ataaacgctg attcacccgg tttaagctgt
     8101 aactgctgag aacctttcca caacgttgca tcgccttcga cgcagaacaa aatggcggca
     8161 ctctgctggc taatggtggt ttctttatca ctaaggtcat gcagcgagaa ggcaaaatca
     8221 tccactggaa tcgggaagtc cagttctgca ccttgtttca ccggctgggt caacaactgg
     8281 ttagccggtt tggcttcgaa tttcacattg gcaaccagtt ccggaatatc aatgtactta
     8341 ggcgtcagac ccgcacgcag cacgttatcg gagttcgcca tcacttccag cgccacgcct
     8401 tgcaggtaag cgtgcggtgt ttcagcgaac aggaacatcg cttcgccagg attcaatttc
     8461 accacattca gtaacagcgg ggagaacagg ccgctgtctt ccgggtaaaa ttcagaaatt
     8521 aaacgaatcg tttgccacgg ttcaccctgc tgactgtcga gcgccgattt taaaatcgcc
     8581 agcgcgcggg atttttcttc accctgcata ttcaacaggc tggcgaacag ttcgcttaaa
     8641 cgttcggcat taggctgttg taaaaagtga gcaattgccg gatgtgcacc tgcgactggc
     8701 tggagtaggg agacaatctc ggaaaactca cgaaacgcgt tcatcgcaag gaaaggcgtc
     8761 agcgcaaaaa ccagctccgg cttgtggtta ggatctttat agttacgctc ggcggcatcc
     8821 atcgggatac ctgcggcatt ttctttggca aaaccgattt cagaattgcg tttgtttgga
     8881 tgaacctgaa tggagagcgg ctgtgctgcg cataatactt tgaacaggaa aggcagttcg
     8941 ccaaagcgtt tggcaacggc ctctccgagc agagtcgatt tatcactctc aatcacatca
     9001 cgcagtgaaa cgatatctcc ggcggcattc tgcactcgtg aactgctttt cggatgtgcg
     9061 cccatccaca gctcggccat cggctggctg gacggatttt ccataccata aagttcagtc
     9121 aacgccgttt tgctgcccca ggcatagttt tgcactgagt taatgagttt ttgcatgatc
     9181 aatccctgtt ttaatgtgga aattaatccc actattaaag caagaatcct acgggaagta
     9241 acctggagcc gcaaaaagtc gtactagtct cagtttttgt taaaaaagtg tgtaggatat
     9301 tgttactcgc ttttaacagg gcaacggaac acccgcccag agcataacca aaccaggcag
     9361 taagtgagag aacaatgtca aacaaaccct ttcattatca ggctcctttt ccactcaaaa
     9421 aagatgatac tgagtattac ctgctaacca gcgaacacgt tagcgtatct gaatttgaag
     9481 ggcaggagat tttgaaagtc gcacccgaag cgttaactct gttggcgcgt caggcgtttc
     9541 atgatgcgtc atttatgctg cgtccggctc accaacaaca ggtggccgac attctgcgtg
     9601 acccggaagc cagcgaaaat gataaatatg tggcgctgca attcctgcgc aactccgaca
     9661 tcgcggcgaa aggtgttctg ccaacctgtc aggataccgg cactgcgatt attgttggta
     9721 aaaaagggca gcgtgtatgg accggtggct gtgatgaagc ggcgctggcg cgcggtgtct
     9781 ataacactta tatcgaagat aatttgcgct actcgcaaaa cgcgccgctg gatatgtata
     9841 aagaggtgaa taccggcacc aatctgccag cgcagatcga tctttatgcc gttgatggcg
     9901 acgagtacaa attcctctgt atcgccaaag gtggtggttc ggcaaacaag acgtatctct
     9961 atcaggaaac caaagcgtta ctaacgccgg ggaaactgaa aaattacctg gttgagaaga
    10021 tgcgcacgct gggtacggcg gcctgtcctc cgtatcatat tgcgttcgta attggtggaa
    10081 cttctgcaga aactaacctt aaaacagtga aactggcttc cgctaaatac tatgacgaac
    10141 tgccaacgga agggaatgag cacggtcagg cattccgcga tgtggaactg gaaaaagaat
    10201 tgctgatcga agcgcaaaat ctcggtctgg gtgcgcagtt tggtggtaaa tacttcgctc
    10261 acgacatacg cgtgattcgc ctgccacgtc acggcgcatc ctgtccggtt ggtatgggtg
    10321 tttcctgctc tgctgaccgt aatatcaaag cgaagatcaa ccgtcagggg atctggatcg
    10381 aaaaactgga acataatcca ggtaaatata tccctgaaga gctgcgtaaa gcgggagaag
    10441 gcgaagcggt gcgcgttgac cttaaccgtc cgatgaaaga gatcctcgca cagttgtcgc
    10501 agtatcccgt ttctacacgc ttatcgctta acggcacgat tatcgtcggt cgtgatattg
    10561 ctcacgccaa actgaaagag cggatggata acggtgaagg gctgccgcag tacattaaag
    10621 atcatccgat ttactacgcg ggtccggcca aaacgccgga aggttatgcc tccggttctc
    10681 ttggcccaac gaccgccgga cggatggatt cttatgtcga tcaactgcaa gcgcagggcg
    10741 gaagtatgat catgctggcg aaaggcaacc gcagccagca ggtgacggat gcctgtaaaa
    10801 aacacggcgg cttctacctt ggcagtatcg gtggtccggc cgctgtattg gcgcagggaa
    10861 gtattaagag cctggaatgt gttgaatatc cggaactggg aatggaagcc atctggaaaa
    10921 ttgaagtgga agatttcccg gcgtttatcc ttgtggatga taaaggaaat gac
//
LOCUS       DAJTNN010000137        10881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTNN010000137 DAJTNN010000000
VERSION     DAJTNN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.137"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(210..2069)
                     /gene="gss"
                     /locus_tag="OO150_003125"
     CDS             complement(210..2069)
                     /gene="gss"
                     /locus_tag="OO150_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5517182.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2274..3140
                     /gene="yghU"
                     /locus_tag="OO150_003126"
     CDS             2274..3140
                     /gene="yghU"
                     /locus_tag="OO150_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5517183.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPICVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3263..3511)
                     /gene="hybG"
                     /locus_tag="OO150_003127"
     CDS             complement(3263..3511)
                     /gene="hybG"
                     /locus_tag="OO150_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5517184.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3524..3865)
                     /gene="hypA"
                     /locus_tag="OO150_003128"
     CDS             complement(3524..3865)
                     /gene="hypA"
                     /locus_tag="OO150_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5517185.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3858..4346)
                     /gene="hybE"
                     /locus_tag="OO150_003129"
     CDS             complement(3858..4346)
                     /gene="hybE"
                     /locus_tag="OO150_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5517186.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4339..4833)
                     /locus_tag="OO150_003130"
     CDS             complement(4339..4833)
                     /locus_tag="OO150_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5517187.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4833..6536)
                     /gene="hybC"
                     /locus_tag="OO150_003131"
     CDS             complement(4833..6536)
                     /gene="hybC"
                     /locus_tag="OO150_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5517188.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6533..7711)
                     /gene="hybB"
                     /locus_tag="OO150_003132"
     CDS             complement(6533..7711)
                     /gene="hybB"
                     /locus_tag="OO150_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5517189.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTREELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7701..8687)
                     /gene="hybA"
                     /locus_tag="OO150_003133"
     CDS             complement(7701..8687)
                     /gene="hybA"
                     /locus_tag="OO150_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5517190.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8690..9808)
                     /gene="hybO"
                     /locus_tag="OO150_003134"
     CDS             complement(8690..9808)
                     /gene="hybO"
                     /locus_tag="OO150_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5517191.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9997..10284)
                     /locus_tag="OO150_003135"
     CDS             complement(9997..10284)
                     /locus_tag="OO150_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5517192.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10403..10881)
                     /locus_tag="OO150_003136"
                     /pseudo
     CDS             complement(10403..10881)
                     /locus_tag="OO150_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
BASE COUNT         2652 a         2989 c         2690 g         2550 t
ORIGIN      
        1 tttaacgata aaaagagtgt tatttcttgt gtatcagaca ataagcattc attcaaaacc
       61 tttagaaata atcaaaacac aggtattcca ctggttaata tattcacata tgaaatgaat
      121 aaatattaga acatataaat attgaatatt ttgattaatc cccgtactga ttattcttca
      181 taatcagtac ggggttgcta caacatgtat tactttttca ccacaattaa cggttcaata
      241 tcactctctt ttttgatgac cagtgattca tcaccgcgca aacacgtccc accgtagttg
      301 ccgcccacgg tgaaggtaca tacctgaatg tatttaccgt ccactttcgg caaacaccac
      361 agttgctgat agatgttttt ctgctcggca aatttaccgc tggttttgtc cagcacctct
      421 tcatgatggc tgaccaggtc gatattgcta ccacagcgac cggcaatcgg tttcactgcg
      481 taacctgttt tcaccaattc atcattaaca gtgaaatcgg tatccagcag gtaacgatgg
      541 tgcgggaaca gcgaccagag gatcggcaga atcgctttgt tgccggggat caccgtccac
      601 agcggctcaa agactagcac ttccgggcgc agcaatacgt cgataagacg cacttcgttt
      661 tgcggatgac cggtacggat tggcaccgca gcaaactcac ggtcgctaac ttcacgaatc
      721 tgatcaaacg cggtttccca cgcccaggtt ttccacacgc agttaaccag tcgcccttcc
      781 ccatcaatca gttgcccggc agcatcccag cccagttcat ccaatccacg caagatacgc
      841 gtttcaaagc ccgcctggtg cagcgcctgc tccataaact gcgcgtgata gttttcctcg
      901 atatctttgt cctgcatgat atggacaaac ggacgtgcac gactgtgttt ccaggcacca
      961 gccaattcgt taatcagccc ttccgccgga ttgaagccgt tgcctttata gccctgctcc
     1021 gcccaacgtt cgaggatcaa gcccgcttcg gtatgacagg aggcggagtc ggcgttgtac
     1081 tcgtaaacct tcaggccacg ctcatccatg cagaaatcca tacgaccagt gatcatatgg
     1141 tgacggcgac gctgccagga gagacgcaaa cgtggccaga ggattttcgg aatgtcgaac
     1201 agcgccagca ggttgtcatc tttcagtacc ttgtcggttg cgtgaagata catcaggtgc
     1261 agctcgttgg tggctttaat tagctcctgc tcggcactct cggtaatggt gtagtaatga
     1321 taaggatcct gattgatcac ctgaccgttg gcctgcacat aggcgttttg cagcggatct
     1381 ttttcatcca gccatttacc gtcaaactgg cctttgtttt ccaggcgcgc tccgctgatt
     1441 ttcagcagct cgcctgcaat ttccggctgc ggtaagctgt attcagtatc ttccgtctgg
     1501 atcatccagc ccagaatggt ggtgtcatca aaagtgtctt tcagggtata gcagccgttt
     1561 tcgaccacca tctccagctc acgcgtccac tgttgccctt gcggcaacgg ggaatgaatc
     1621 acgttctgtt ccgcaatacg gactttgttg ccatgcaatt gggtaatgat ggcgacatgg
     1681 ccagtgtctt taaattcacc gcctttatcc cagataagaa gcgcacccgc gaccggcgca
     1741 cgcggcgagc cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac cacttctcgc
     1801 aggaagcgca gcgagaaaat ctcccacgcc atacccacgt cagtaaagac cacaccgtaa
     1861 ttcagaaaga gaaaacggcg agcaaattca acgcattgcc acttgtggcc catatattcg
     1921 tcgtcgatat agctacggaa tacggcgtca tcttcgtatt cctgcggatc gagagaactg
     1981 taatctgaag agtagattgc taccccacct ggggcgtagc ccaataatgt cccgaacggg
     2041 gcatcctggc tggtcgttcc tttgctcatc actttacctt taacaataca acctaagcag
     2101 ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca ttaaccggac agaggtcaga
     2161 ctaatcatac acctcatcgc agcggctggc gcaaagggtt aaaaaattca cattctgtac
     2221 agcaagtgac ctgccacact gcttcaacac taccactcag aaggcaactc actatgacag
     2281 acaatactta tcagcccgcg aaagtctgga cgtgggataa atccgctggc ggcgcgttcg
     2341 ccaatatcaa tcgcccggtt tctggtccga cgcatgaaaa aacgctgccc gttggcaaac
     2401 acccattgca actttattcg ctgggaacgc cgaacggtca gaaagtaacg attatgcttg
     2461 aggagctgct ggcgctgggc gttactggtg cagagtacga cgcctggctg attcgtattg
     2521 gcgatggcga tcaattctcc agcggctttg tcgaagtgaa cccaaactcg aagatcccgg
     2581 cgctgcgcga tcatacgcat aatccgccga tctgtgtgtt tgaatctggt tcgatcctgc
     2641 tttatctggc ggagaaattt ggctacttcc tgccgcagga tttggcaaag cgtactgaaa
     2701 cgatgaactg gctgttctgg ttacagggcg cggcaccgtt cctcggcggt ggttttggtc
     2761 acttttacca ttacgcaccg gtaaagattg agtacgccat caaccgcttt accatggaag
     2821 ccaaacgtct gctcgacgtg ctggataagc aactggcgca gcataagttt gttgcgggcg
     2881 atgagtacac cattgcggat atggcgattt ggccgtggtt tggcaacgtg gtgttaggtg
     2941 gtgtgtatga tgccgctgag tttcttgatg cgggcagtta taagcatgta caacgctggg
     3001 cgaaagaagt aggcgaacgt ccggcggtga aacgtgggcg tattgttaac cgcaccaacg
     3061 gaccgctgaa tgagcagttg catgagcgcc atgacgccag tgatttcgag acgaatacgg
     3121 aagataagcg tcaggggtaa gggttggtgt tcgtcgcagc aagccatcca ggccggataa
     3181 ggcgttcacg ccgcatccgg caatcgtgca caatgcctga tgcgacgctg ccgcgtctta
     3241 tcaggcctac gaaagctaat catcacgcgc tggtaatgtc gtaatccatt tggcgcagtg
     3301 cgtctaatgt ggctttggct tcatcttcgt cgatgatgct catggcaaat ccgacgtgta
     3361 ccagcaccca ctggcccagt agatcggcag ggttaccttc acaaatcagg gcgatattca
     3421 cgtcgcgctt gataccacat acttcaacct gcgcaagctg gtgaatatct tcaccgacag
     3481 ccagcacctg gcctggaacg ccaatacaca ttattaactc cggttattca acttcaatac
     3541 ttttgacgat cagcgaatcg ccggtatcga cacgcaaccg ctcgccgtga cagagcggac
     3601 actgcgcatc gtgctgatga atctccacca cctggctgca atcccagcac caggcctggg
     3661 cgggtttata gacgatatgt aaatcgcacc cttgcgccac cgttccgtgg cagacaattt
     3721 caaaactaaa acggacggcg ctctcctcaa cgcaggagag cgcgccaatt tccagccaca
     3781 cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg ttggataatt tcaacggcgc
     3841 tctggcaaag cgacaactca tgcattttcg ccactcctgc gaccaaacag cagggcgcga
     3901 cgccctgcgt gtggtacatc cggattcgtg actggcagcg aaaggatcat tcgtgcgcag
     3961 tcatccgtca ggcgttggcc ctcttcaatc gacatactgt gcgaaagcgg tgacatcagc
     4021 gaacaggaga ggtattgcga aacaccgtcc agttcgccaa cggtaaaggt catggtgcca
     4081 tacggcagtt gcagaccaat tttttcactc actttgcgca gcggccagag ttgatccggg
     4141 ccggggaaga taactgcact cagcatccac ggggtgatca cacaccccgt ccactgacct
     4201 tcgaacagcg taaaatcaga aacatacacc ggcattgaag gatgcagaaa agagagatcg
     4261 tgcatcgaac gccgggcaat ttcttcaaac gctgcctgta cttgcgcctt cggggaggtc
     4321 tggaaacctg ctatctcttc agtcatgaat cgcctcccgt gggatggctt ccacgcctga
     4381 ttcgcgcagc gcagccagaa cctgctcaag cgcaggttca atcattgctt caaccgtcgg
     4441 cgttaagccg atgtgtggct ccagcgattc cgggatcacg ccgaccaggg tcagcttttt
     4501 cggaaactca ccggtgaagc gcagggccga caatacgtcg gccaggccaa gctgatgcgg
     4561 agagattttg ttggtaaaca acgccggaac ttcttcatcc cgcaggatca tcatcgttcc
     4621 cggcgtgttc tttttcgaca caatggcatc cgcaataatc agatgatcgc gatttgccat
     4681 gtcgccaagc agctccattc ccgccgtgcc gccatcgagg atctcaacat aatccggcag
     4741 aatgtatcgt tgctctaacg cttcgacaat ccgcacaccg atggcttcat cggtcagcaa
     4801 aatattgccg acccctaaga ctaaaatacg cattacagaa ccttcactga aaccacttcg
     4861 ttgccgtcgg catccactac gtgtaccgca caggccatgc acgggtcgaa ggagtgaatg
     4921 gtacgcacca cttccagcgg tttattcgga tcagcaaccg gtgtacccac cagcgactgc
     4981 tcgtaaggac cgacgtcatc attgaagtta cgcggaccag agttccaggt tgatggaaca
     5041 actgcctggt agttactgat aataccgtct ttaataacca tccagtgaga gagcataccg
     5101 cgcggcgctt cgaggaagcc aacacctttg aactcacccg ttgccggaat gttcggtttc
     5161 acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca gtgcactgta ttgattttgc
     5221 aggatatcct gcaattcgca gcagtgaacg gtacgaccaa taatacggcc cagcgtggag
     5281 tgcagctgcg ccacttccag cgtgttgcca gtcagtttct gataaatcgc aacgatttca
     5341 ttcagtttgt tctgggtaga ttcgcgacct gccgccagtt tcaccagcat attagccagc
     5401 ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt tcacccagga atatttcccg
     5461 tcgtcagacc aaccatcata agccggaatg gtggtacctt cccacggtgc ctgcggcgct
     5521 tcgtctttat accaggagtg cttcgcgctt tcctgaatgc ctttgatcag gtattcatcg
     5581 gaatgagaag tgatcggacg atacgaggac agatccgcat tctcaatgta gccgcccggg
     5641 aacaggaagc tgccgttttt actgtcggtc gggaattccg gcacgctcag gtagttcacc
     5701 gcacctttac cgcgcgtcag ccattccggg tagaacgcgg caataactgc ggtatcaacc
     5761 ttataaacct gttcaacaaa gtcgctcagc ttgtcgatga aagacttgat gtacatcagg
     5821 cgctcaaggt tcagcacgcc caaaccgtcg aggttgatcg ggttcgcgac accacctacc
     5881 gccaggttct gaatgtgcgg cgttttaccg cccagcagcg ccacgacgcg gttagcgtca
     5941 cgctggcact ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac ttccggcggc
     6001 agtttcatcg ccgggtgacc ccagtagcca ttggcgaaaa tacccaactg accgctggca
     6061 accagatctt tgatcttgtt ctgaacttta gtgaactctt ccggactgtt cagatgccag
     6121 gtcgaaacgc ctttcagcat ttcggaggct ttggttgggt cagcttgcag tgcagaagtg
     6181 atgtccaccc agtccagcgc cgaaagctga tagaaatgaa cgatatggtc atgcgtggtg
     6241 tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc gatattcagc
     6301 gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg tagtacatac gccacagata
     6361 cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc ttccatgccg
     6421 cgccacatgg taccggaagc ccatgctttc gaaacgacgc cattttcgat ttcgcaatcg
     6481 atgcgtaaat gcccctcaat acgggttacc ggatcaatag taattctctg gctcatgctt
     6541 tgctcgcctc atgacgatta tgatcgtttt gttttaaagg aggaagtatc ggcagtagac
     6601 gaatgagtac gatgtaagcg caaatctcaa tagccacaaa accaatagaa atcaacagtt
     6661 cttccctggt cgggaagtag gcgtaaccgc cgcccgggtt gaatgccacc agcgaatagg
     6721 tcagacgcca ggttgcacaa cctaacagtg cgctcagtgc tgacaggaac agcatgcggg
     6781 aatcattacg cagcttcgcc acacgcagaa cgaccagcgg gaagagcatc agcaggactt
     6841 caatccagaa catcacggag tagaagtcac cggcaaacgc taacgacagc ttgtcgcgat
     6901 agataagctc gccaaagcgc agcacgataa aaatcgccag caacacactg atggtgttgg
     6961 tcagcttgac gaacagactc ttttcatccg gaccgttgcc acgcagaccc gcctgcacca
     7021 gcgaaccttc aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc agcgagaaca
     7081 gcggcaacat ttcatagctc tgccacaacg gatgcacctt gtagcccgcc gagatcatca
     7141 gtgaccccat tgaagactgg tgcatggtcg gcaacagcgc gccaagtgca atgatgaaga
     7201 acatcacctt gttcagacgc ttgagagaaa ccttccagcc cagacgttca aacagtgccg
     7261 gagcaaactc cagtgccatc acgccgatat agatggtcat acagaccgcc gtctcgaaca
     7321 gtaccgagtt cacgttgaag tgacccggaa tgtagaagta cggcaggttc cagtagcgac
     7381 ccacgtcgat agtgatcgac aagccaccca gtgagtaacc aaacagactc gccaacagcg
     7441 ccggacgcac cagcggatgg tattgcccac ggttaaagac gtataccgcc cacgccagcg
     7501 cccagccgcc acaggcaaag ccggtaccaa tcagcaggtc aaacgcgatc cacacgcccc
     7561 acgggaagcc gccgttcagg tcagagacag agcccagacc gaacaccaga cgcttcacaa
     7621 tcaggagcat acagatgacg attaacggtc caaaaatcat gaccggttta ctgatgattt
     7681 tgccgcccag cggttgtgga tcatgactca tgatcgtctc ctccgtcgtg atggtcgttt
     7741 ttggtgttgc gacgaaccag cacggttaag cccgccagca cagccagtgg tagcatcatg
     7801 cctttataca gggtgtgttg aatattttcg gaacgcgcac cggtagaaag atcgtccagt
     7861 ttcggcaggt cgagattttc ataaggcaca cccgtcagta ccagaacctg agtaccgccg
     7921 ccctctttct cgccgtacag atgcggataa tatttcggca ccgtatgcag gtaagtgtcg
     7981 ccagatttca gcgtctgacg cggatagtgg tattcgctgc caggcttcag cgccagacgt
     8041 tttttcgcct ccgccatcag ctcttcacgc gtaccgaaaa tcaccgcgcc cgccgggcac
     8101 acttctacgc agccaggcag accgccttta tcgagacgtt ccacaccttt ctggttgcac
     8161 agctcgcact tatgcagcgc accaaacggg ttgttgtagt cgtacttcgg cacgttgtac
     8221 ggacaggcga ccatgcagta acggcaaccg gtgcacacgt ctttgtcgta atggacaatg
     8281 ccggttttcg gatctttttt cagtgcagag accgggcaca cagagacaca gttcggatcg
     8341 acgcagtgca tacactgttt cttaatgtac gcgtagccgt tctcctcctg gtctttgttg
     8401 acccctgtgc cgctggtcca cacctgaatg atgttattgg tatacggcga cagtttgtcg
     8461 ttgttcgacc aggtctgttc cccttgcggg ttacgttcag ggaagttgat atcctgacac
     8521 ttggtgacgc aagcctggca gcctacgcac aaggtcgagt cgtacaacat ccccagcgat
     8581 cccggaattg gcgggcggtt ttcagcagcc gcatgactga cagacggcag cgcgcccgtc
     8641 agcaatgccc cgcaggaggc tgctttaata aaattacgtc tgttcacggt tattctcccc
     8701 gtgagtcagc gttatctttc ttttgctgac gacccagttc acgcaccgcc atcacgctga
     8761 caccggcaac cagcccaacc acaccgccga gcaaaccaat agcgcctgca gagacgttac
     8821 cgccctcttt agcgttaaca tccggtttct gtgaacgcgg agtctgattt tcgacgttgg
     8881 caagctgatg gatgccttta tggaagccga taccttcttc gttacagcca tagcatgggt
     8941 gaccaatcgc taccggccac acgccgccaa catcgcagaa ttgcagcgtt gagcagttgc
     9001 cgtaagtttc tggcccttta cagccgaggt ggtacaggca ccagccttca cggtggcctt
     9061 catcaccgaa ctctttggca aaacgaccag catcgaagtg cgggcgacgt tcgcagtgtt
     9121 cgtgaatcag acggccatag gcgaaggtcg gacgattttt gtcatccagt ttcggcggtt
     9181 tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc ggcgggcagc
     9241 ccggaatatt gataacggtt ttgcctggca gaacttcttg caggctgact gcgccagttg
     9301 ggttaactcc agctgcggca acaccgcccc acgcagagca ggaaccgata gcgataatcg
     9361 ctgctgcgcc ttctgccgct ttgcggatgt gatccacaat cggctcacca gcaaccatgc
     9421 aataaatacc gttatctttt aatgggatgg aaccatccac aaccaacaca tactgccctt
     9481 tgtacttctc aagtgcgtta tgtttgttct cttcgacctg atgaccgaag gcggcggaaa
     9541 gcacttcgtg atactccaga gagatagtct ccagcacgag gttttctacc gttggatgcg
     9601 ttgcacgaag cagagattcc gtacaaccgg tgcactcctg cgcgccaatc cagataactg
     9661 gcggacgctg cgggttagta accgattcgg ccatctctgc agcggctttg ctacttaacc
     9721 ccatggtggc ggctaatgct gcacaaagct tcatgaaatc acgacggtta atgccgtgag
     9781 aatggatgag ggtgttatct ccagtcattt atagttattc cgttgcgaag acctggcata
     9841 tattttgcct caatcgcaaa atcaataatg cgatcgatgc gccatttacc acacatttat
     9901 tatggttatc gatatcatga tactgcggcg gggcgctaaa acgaagggaa gaagcattag
     9961 tgtaattaat taaagcaaga taatacgtat gtttgatcaa ttttcgaggc gataaccggc
    10021 cataaaaaag cgcaacgtag aacaggaatt attctcacga aagaaatcca ttaccatctc
    10081 tttatccagt ccatagcggc gcgtcagaat acccgcttcc caggcgctaa gctggcgatc
    10141 tccggtcttt tcgtacatcc ggtgtgagta gcctaataca aaaccgcgtt tataatcggc
    10201 acagaatttt gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc ccgccattaa
    10261 gcctttacca aaatggttat tcatcgttca cctcattcgc tatatattat catatatagc
    10321 aatattttag gcaaagaacg acttttgcgc acgctatttt ttcatgcttg tttaagacgt
    10381 aatcccgtaa gagataaacc catcaggaga gatatttatc gaaccatttc agcgtccttt
    10441 gccaggcaag atcggcggca gatttgtcat aacgaggcgt ggaatcatta tggaatccgt
    10501 gattaacccc cggatagata tacgcctcat agaccttatt attggctttc aacgccgcct
    10561 cataagcggg ccagccctcg ttgattcggg tgtccagttc cgcgaagtgg agtagtaaag
    10621 gcgcttcaat cttcgccaca tcggcagtgg gtgcctgacg accataaaac ggcaccgcgc
    10681 aggccagttc cggatacgcg acagccgccg cgttcgatac accaccgcca tagcaaaatc
    10741 cggtaatacc cacttatgcc tgtcgcttgc ggatagcgtt gcataaactc aattgcggca
    10801 aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca
    10861 tcatttcccg gataacctcc a
//
LOCUS       DAJTNN010000138        10840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTNN010000138 DAJTNN010000000
VERSION     DAJTNN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.138"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(257..463)
                     /locus_tag="OO150_003137"
     CDS             complement(257..463)
                     /locus_tag="OO150_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5517193.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            775..864
                     /gene="kdpF"
                     /locus_tag="OO150_003138"
     CDS             775..864
                     /gene="kdpF"
                     /locus_tag="OO150_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5517194.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            864..2537
                     /gene="kdpA"
                     /locus_tag="OO150_003139"
     CDS             864..2537
                     /gene="kdpA"
                     /locus_tag="OO150_003139"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5517195.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALSADSSINMEGKESRFGVQVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2560..4608
                     /gene="kdpB"
                     /locus_tag="OO150_003140"
     CDS             2560..4608
                     /gene="kdpB"
                     /locus_tag="OO150_003140"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR5517196.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADIAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSITNDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4617..5160
                     /gene="kdpC"
                     /locus_tag="OO150_003141"
                     /pseudo
     CDS             4617..5160
                     /gene="kdpC"
                     /locus_tag="OO150_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            5153..7837
                     /gene="kdpD"
                     /locus_tag="OO150_003142"
     CDS             5153..7837
                     /gene="kdpD"
                     /locus_tag="OO150_003142"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR5517197.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTDAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNALGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7834..8511
                     /gene="kdpE"
                     /locus_tag="OO150_003143"
     CDS             7834..8511
                     /gene="kdpE"
                     /locus_tag="OO150_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR5517198.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8727..8984)
                     /locus_tag="OO150_003144"
     CDS             complement(8727..8984)
                     /locus_tag="OO150_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517199.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            9107..>10840
                     /gene="speC"
                     /locus_tag="OO150_003145"
     CDS             9107..>10840
                     /gene="speC"
                     /locus_tag="OO150_003145"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5517200.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPS"
BASE COUNT         2419 a         2759 c         2960 g         2702 t
ORIGIN      
        1 gccggttttc cgctcatttc tcatccttgt tttatcgggt tatttgtctg ctgacaggca
       61 aaaccctaca gatttataag aaagccttaa gtgagatatc catcagatag aaacaattag
      121 gaaaatattt atatattttt cctgaatata tttcagctgg gtaataccat atctaaatat
      181 ttacctgaac atcttcctaa taaaaaaacc gccagcatct ccactagcgg ttttcaatgt
      241 attcaaaact cggcaatcag taaaaatcac cggttgcctt ttcggcctgt tccatccaaa
      301 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      361 ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca tatgtacggc
      421 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      481 gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      541 gccttttgtg taattttact actcatccga ccacttattt ttgcttattg atggtttatt
      601 tacattcact ttgtaattaa gttgcacaaa agttaaatta atgctaaaca ttagttaaac
      661 catggctttt gccattttta tactttttta caccccgccc gcagattttt gcgaaatctt
      721 tgcagccaga attctactct tccggtatca cttttaggcc actggaggtg cactgtgagt
      781 gcaggcgtga tagccggcgt gttgctggtg tttttattac tgggttattt ggtttatgcc
      841 ctgatcaatg cggaggcgtt ctgatggctg cgcaagggtt cttactgatt gccacgtttt
      901 tactggtatt aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg
      961 acattcctct tccaggtaca gcgggcgttg aacgtatact ctttcgttta cctggcgtct
     1021 ctgaccatga gatgaactgg aagcaatatc tttgtgccat tctcggcctg aacatgctgg
     1081 ggctggcggt gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc
     1141 agttgccggg gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata
     1201 ccaactggca atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcaggct
     1261 taacggtaca aaactttctt tctgccgcca gcgggattgc ggtgattttt gccttcatcc
     1321 gtgcgtttac tcgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca
     1381 tcacgttatg ggtgctagtc cctgtggcgt tgttgatcgc actgtttttt attcaacaag
     1441 gtgcgctgca aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc
     1501 tgttacccat gggaccagta gcttctcagg aagcgatcaa gatgctcggt actaacggcg
     1561 gtggcttctt taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact
     1621 tcgtgcagat gctggcgatc ttcttgatcc caacagctct gtgctttgcc tttggtgaag
     1681 tgacgggcga tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca
     1741 tctgcgtagg cgtggttatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctca
     1801 gcgcagacag cagcatcaat atggaaggta aagagagccg tttcggcgtg caggtcagta
     1861 gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc ggtgattgcg atgcatgatt
     1921 cgtttaccgc tctcggcggc atggtgccga tgtggctaat gcaaattggt gaagtggtgt
     1981 tcggcggtgt cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta
     2041 ttgccgggct gatgattggt cgtacaccgg aatatctggg taaaaaaatc gacgtacgcg
     2101 agatgaaact gactgcactg gcgattctgg ttactccaac actggtactg atgggcgcgg
     2161 cgttggcgat gatgaccgac gccggacgta gcgccatgct taaccctggc ccgcatggtt
     2221 ttagcgaagt gctgtacgcc gtgtcgtccg ccgctaacaa caacggcagc gcctttgccg
     2281 gattaagcgc caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc
     2341 gcttcggggt gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc
     2401 aacccgccag ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg
     2461 gcaccgtgtt gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg
     2521 cggaatatct ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg
     2581 ctattcgaac caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg
     2641 caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc
     2701 tgtattagca tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca
     2761 attagcggct ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa
     2821 ggccgcagta aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgcccgc
     2881 aagctgcgtg agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt
     2941 aaaggtgaca tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt
     3001 gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt
     3061 gaatccggcg gcgattttgc ctccgtcacc ggcggcacgc gtattctttc tgactggctg
     3121 gtgattgagt gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg
     3181 gaaggcgcac agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct
     3241 ctgaccatcg tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt
     3301 aatgccgtga gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt
     3361 ggcggcctgt tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg
     3421 attgccacca gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat
     3481 aaaaccggca ccatcacact cggtaaccgt caggcgtcgg agtttatccc tgcgcagggc
     3541 gtggatgaaa aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg
     3601 gaaggccgca gtattgtgat cctcgccaag caacgtttta acctgcgcga gcgcgatgtg
     3661 cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaac
     3721 atcgacaacc gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tgttgaggct
     3781 aacggcggtc acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcagggg
     3841 gccacgccgc tggtggtggt ggaaggttcc cgtgtgctgg gcgttattgc gctgaaagat
     3901 atcgtcaaag gcggtattaa agagcgcttc gcccagctgc gcaaaatggg cattaaaacg
     3961 gtgatgatta ccggcgataa ccgtctgact gccgccgcca ttgctgcgga agcgggcgtc
     4021 gatgattttc tcgccgaagc gacaccggag gccaagctgg cattgattcg tcagtatcag
     4081 gcggaaggcc gtttggtggc gatgaccggc gacggcacca acgatgctcc ggcgctggcg
     4141 caagcagata tcgcggtggc gatgaactcc ggcacccagg cggcgaaaga ggcgggcaat
     4201 atggtcgatc tcgactctaa cccgaccaag ttgatcgagg tggtgcatat tggcaaacag
     4261 atgctgatga cccgtggctc gctgaccacc ttcagcatta ccaacgatgt ggcgaaatac
     4321 ttcgccatta ttccggcggc gttcgcggca acgtatccgc agttaaatgc gctgaacatc
     4381 atgcgcctgc attcgcccga ctccgccatt ctcagtgcag tcattttcaa cgccttgatt
     4441 atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct
     4501 gccatgttgc gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt
     4561 ggtatcaaag tcattgattt actgctgacc gtttgcggtc tggtgtgagg tttaccatgc
     4621 gtggcttacg tccggcatta tcgacattta tctttctgtt attgattact ggcggcgttt
     4681 acccgctgct gaccaccgtg ctggggcaat ggtggtttcc ctggcaggcc aacggttcgt
     4741 tgattcgtga aggtgatacg gtgcggggtt cggcattaat cgggcagaat tttaccggca
     4801 acggcagaaa tgccctataa tccacaggct tctggcggga gcaatctggc ggtcagtaac
     4861 cctgagctgg ataaacaaat agccgcacgc gttgctgcat tacgggccgc taacccggat
     4921 gccagtacga acgttccggt tgaactggtg acggcatcgg caagcgggct ggacaataat
     4981 atcaccccgc aagcggcggc ctggcagatc ccacgcatcg cgaaagcgcg taatctcagc
     5041 gttgaacagc tcacgcagct gatcgcaaaa tacaaccaac aaccgctggt gaaatatatc
     5101 ggccagccgg tcgtcaacat tgtcgaactc aatctggcgc tggataagct tgatgaataa
     5161 cgaaccctta cgtcccgacc ccgatcgtct gctggaacaa actgccgcgc cgcatcgggg
     5221 gaagctgaaa gttttcttcg gtgcctgtgc aggcgtcggg aagacctggg cgatgctggc
     5281 agaagcccag cgactgcggg cgcaagggct ggatattgtg gttggcgtgg tagaaaccca
     5341 cgggcgaaaa gataccgacg caatgctgga agggctggct gttctgccgc caaaacgcct
     5401 ggcgtaccgt gggcggcaca tctgcgagtt tgacctcgat gccgccctcg cccgccgtcc
     5461 ggcgctgatc ttaatggacg aactggcgca cagtaatgcg ctaggttctc gtcaccctaa
     5521 acgctggcag gacatcgaag agctgctgga agcaggcatt gatgttttca ctaccgtcaa
     5581 cgttcagcat ctggaaagtc tgaatgatgt ggtcagcggc gtcaccggaa ttcaggtacg
     5641 ggaaaccgtg cccgatcctt ttttcgatgc cgccgacgac gtggttctgg tggacttgcc
     5701 cccggacgat ctgcgccagc ggctgaaaga aggcaaagtc tatattgccg ggcaggcgga
     5761 gcgcgccatt gaacattttt tccgcaaagg caatctgatc gccctgcgcg aactggcact
     5821 gcgccgtact gccgatcgcg ttgatgagca aatgcgcgcc tggcgggggc atcctggcga
     5881 agagaaagtc tggcacacgc gcgacgcgat ccttttatgc atcggccata acaccggtag
     5941 tgagaagctg gtccgcgcgg cggcgcggct ggcgtcacgg ctgggtagcg tctggcacgc
     6001 ggtgtatgtt gaaacccctg ccctgcaccg cttaccggaa aaaaaacgtc gggcaattct
     6061 cagcgcctta cgtctggcgc aggagctggg cgcggagacg gcaacacttt ctgatccagc
     6121 ggaagaaaaa gcagtagtgc gttatgcccg tgaacataat ctcggcaaga ttattctcgg
     6181 tcgcccggcc tcgcgccgct ggtggcgtcg ggaaacgttt gctgaccgac tggcgcgcat
     6241 cgcccccgat ctcgatcagg tgctggtcgc gcttgatgaa ccacccgccc gcacgattaa
     6301 caacgcgccg gatagccgct cttttaaaga caagtggcgg gtacagattc agggatgcgt
     6361 ggttgccgcc gcgttatgcg ccgttatcac tttaattgcc atgcagtggc tgatggcgtt
     6421 tgatgccgcc aacctggtga tgctgtatct gcttggcgtg gtggtggtgg cgctatttta
     6481 tggacgctgg ccttcggtgg ttgctaccgt cattaatgtg gtgagtttcg atctcttttt
     6541 tatcgcccca cgcggcacgc tcgccgtctc tgatgtgcaa tatctgctga ccttcgcagt
     6601 gatgttaacc gtcgggctgg tgatcgggaa ccttactgct ggcgtgcgtt atcaggcgcg
     6661 ggtagcccgt taccgcgagc aacgcacacg gcacttatat gaaatgtcga aagctctggc
     6721 ggtgggccgc agtccgcagg acatcgccgc caccatcgaa caatttattg cctccacgtt
     6781 tcatgcccgc agtcaggtgt tgttgcccga tgacaacggt aaattgcagc cgttaacaca
     6841 tccgcaagga atgacgccat gggacgatac catcgcgcag tggagttttg ataaaggcct
     6901 gcctgcgggc gcgggcaccg acacgttacc cggtgtaccg taccagattt tgccgctaaa
     6961 aagcggcgag aaaacctacg ggctggtggt ggtggagccg gggaatctgc gccagttgat
     7021 gatcccggaa cagcagcgcc tgctggagac gtttacgctg ttagtcgcca atgcccttga
     7081 gcggctgacg ctaaccgcca gcgaagaaca ggcgcggatg gcaagcgaac gtgaacagat
     7141 ccgcaacgcc ctactggcag cgctttcgca tgatttacgc acgccgctca cggtgctgtt
     7201 cggtcaggca gaaatcttaa cgctcgatct ggcaagcgaa ggatcgcccc acgcccgcca
     7261 ggccagcgag attcgtcagc atgtgctgaa cactacccga ctggtgaata atctactgga
     7321 tatggcgcga attcagtccg gcggctttaa tttgaagaaa gagtggttaa cgctggaaga
     7381 agtggtcggt agcgcgctgc aaatgctgga accgggttta tcgtcgccca tcaatctttc
     7441 tctgccagaa ccgctgacct taatccacgt tgacgggcca ctctttgaac gggtgctgat
     7501 taatctgctg gagaacgcgg tgaaatatgc gggtgcgcag gccgaaattg gtatcgatgc
     7561 ccacgttgag ggcgaaaatc tacaactgga tgtctgggat aacggccccg gtcttccgcc
     7621 aggccaggag cagacgatat ttgataagtt tgctcgcggg aataaagagt cggcagtacc
     7681 gggggtaggg cttggactgg caatttgtcg ggcgatagtg gatgtacacg ggggcactat
     7741 taccgcgttc aaccgaccgg aaggtggtgc ctgttttcgt gttacacttc cccagcaaac
     7801 tgcccctgaa cttgaagaat ttcatgagga tatgtgacaa acgttctgat tgttgaagat
     7861 gaacaggcta ttcgtcgctt tctgcgcacg gcgctggagg gcgacgggat gcgcgtctat
     7921 gaggccgaaa cgctgcaacg cggcttgctg gaagcggcta cccgtaagcc agatttgatt
     7981 attctcgatc tcggcctgcc cgatggtgat gggattgagt ttatccgcga cctgcgccag
     8041 tggagcgcgg tgccggtgat tgtgctttcc gcacgcagcg aagagagcga caaaatcgcc
     8101 gcgctggatg ccggagcgga tgattatctg agtaagccgt ttggcattgg cgaattgcag
     8161 gcccgtctgc gcgtcgcttt acgccgccac tccgctaccg ccgcgcccga tccgctggtg
     8221 aaattttccg atgttaccgt cgatttagcc gcccgcgtga ttcaccgggg tgaggaagag
     8281 gtgcatctca caccaattga gttccacctg ctggcggtgc tgctcaacaa tgccggaaaa
     8341 gtactcaccc agcgccagct ccttaaccag gtgtgggggc caaacgcggt cgaacacagt
     8401 cactatttgc gtatttatat gggacatctg cgacaaaaac tggaacagga tcccgcccgc
     8461 ccacgccatt tcattactga aaccggtatt ggctatcggt ttatgctttg aatattaatt
     8521 ttaatacagc ctgcctttta ttaattaaag ccgtaataat aaatacggct ttttatctta
     8581 aacaacacac aaaaataaca attcaatatt ttatattact gagtaaaaag ctcatcatta
     8641 aataaattaa gattgatcat tttttattga tcaccttcac agttcaaccg tatttcctgg
     8701 atagaatatc ttcaccttca ttcacatcag gaaaggtaaa ttaaatggaa aataacagcc
     8761 gcactatgcc ccatataagg cggacaactc atattatgaa gtttgctcat cgcaatagct
     8821 tcgactttca cttcttcaat gcccgttagt ctaccgacta agggcacttc agcgtacagg
     8881 tcttcctgac tctctgtatt acaggcatat gcctgtattc cgcagtgctc attctctgtt
     8941 aacccttgta ttatcagcca ccggactggt aagggtttct gcattctgaa aaaagcaatc
     9001 tacctgattt atttatcagc ggggtaactt tgcttttttc cggcacgatc gatttctcat
     9061 tcgagaaatt gaggacctgc tattacctaa aataaagaga tgaaaaatgt caaaattaaa
     9121 aattgcggtt agtgattctt gcccggactg ttttaccacg cagcgagaat gtatctacat
     9181 taatgaaagt cgtaatatcg atgtggcggc aatcgtttta tcgctcaacg atgttacatg
     9241 cggaaaactc gatgaaatcg atgccacggg ttatggcatc ccggtattta ttgctactga
     9301 aaatcaagaa cgtgtacccg cagagtattt gccctgtatt tcgggtgtct ttgagaattg
     9361 cgaatcgcga cgagaatttt atggtcgtca gttagaaacc gctgccagcc attatgaaac
     9421 tcaactgcgc ccacctttct tccgcgcact ggtcgattat gtcaatcaag gtaacagcgc
     9481 gtttgattgc cctggtcatc agggcggcga atttttccgt cgccatccgg cggggaatca
     9541 gtttgtggaa tactttggtg aggcgctgtt ccgttccgac ttgtgcaacg cagacgtagc
     9601 aatgggtgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
     9661 agtgttcaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
     9721 ggttttaaac gccctgctga caccgggtga tctggtgctg tttgaccgca ataaccacaa
     9781 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaaccgc
     9841 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
     9901 gcgtgagctg atcgcggaag tcgcaccgca gcggacaaaa gaggctcgtc ctttccgcct
     9961 cgccgttatt cagctaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
    10021 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggtcg gctatgagca
    10081 atttatcccg atgatggctg actgttcacc gctgttgctg gatcttaatg agaacgatcc
    10141 gggcattctg gttacgcaat ctgtgcataa acagcaggct ggtttttctc aaacttcaca
    10201 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
    10261 gaacaacgcc tttatgatgc atgcctccac cagcccgttc tatccgctgt ttgccgcact
    10321 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
    10381 ggtaaatggc attaatgccc gcaagctgat cctcgataac tgtcagcata ttcgtccgtt
    10441 cgtacctgaa ctagtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
    10501 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cactcttttg aaggctatgc
    10561 agagaatcaa tactttgtcg atccatgcaa actgttgctg acaaccccag gtattgatgc
    10621 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
    10681 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
    10741 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
    10801 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
//
LOCUS       DAJTNN010000139        10651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTNN010000139 DAJTNN010000000
VERSION     DAJTNN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.139"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="OO150_003146"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="OO150_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5517201.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1606..1929)
                     /locus_tag="OO150_003147"
     CDS             complement(1606..1929)
                     /locus_tag="OO150_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5517202.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OO150_003148"
     CDS             complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OO150_003148"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5517203.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYKAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OO150_003149"
     CDS             complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OO150_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5517204.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLHGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OO150_003150"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OO150_003150"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5517205.1"
                     /translation="MTENTVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREALAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OO150_003151"
     CDS             complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OO150_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5517206.1"
                     /translation="MNTVCTHCQSINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7529..8566
                     /locus_tag="OO150_003152"
     CDS             7529..8566
                     /locus_tag="OO150_003152"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5517207.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAELVQSITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OO150_003153"
     CDS             complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OO150_003153"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5517208.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9608..9991
                     /gene="grcA"
                     /locus_tag="OO150_003154"
     CDS             9608..9991
                     /gene="grcA"
                     /locus_tag="OO150_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5517209.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="OO150_003155"
     CDS             complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="OO150_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5517210.1"
                     /translation="MTPTLLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2650 a         2703 c         2736 g         2562 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgcc tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcat ggttaatgtt gcataataat acgtaacaaa tgtataacat
      241 tttctttacc aaaagaaata aacaaaagtg accaacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcga ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac attttcttcc cttccgggaa
      481 cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac tgctagccct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 attagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc
     1261 gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata catcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt
     1561 tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat ccttccagct
     1681 ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca aactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag
     1981 gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac
     2041 cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga tggtggtatg
     2101 ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agctccagtt gtggatcgtg
     2161 gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt
     2221 gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc
     2281 tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac ggctcaggaa
     2341 aagacgcaga ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc
     2401 ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga cctttttccc
     2461 ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga agtatggcgt
     2521 acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct tgttcaacag
     2581 actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc
     2641 ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat cgttcttgat
     2701 ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat
     2761 attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga taagatgata
     2821 acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct
     2881 ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg
     2941 ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct gcgccatgcg
     3001 gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg
     3061 atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag ctttatacaa
     3121 cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga caatgcaaat
     3181 gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg cgggagcgta
     3241 aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg
     3301 atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag
     3361 gatcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt
     3421 agtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt tagcgtaagc
     3481 ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggctagcgcc
     3541 accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc
     3601 gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt gagatccgag
     3661 cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc acgcgtgacg
     3721 ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat ttcccgatcg
     3781 tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag
     3841 tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag ttgtggctca
     3901 tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct
     3961 tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc ttcaaacggc
     4021 gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga
     4081 atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc
     4141 tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt
     4201 ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg
     4261 acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg
     4321 aaaaccggat cgtgcccaac cacaacccgc aactcctgag cgccagcacg gttagccata
     4381 ctttgcacca acaggccgtg gatccgcgcc tgcggccagg ccatttttac gcgatcgaaa
     4441 atggcgtttg ccgcttgctg gacttcattg gctgtacgca agtacagcat gacgccctga
     4501 acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg ataaccaatc
     4561 tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg gagcgtgttc
     4621 atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg
     4681 gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc
     4741 aacgccggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat atgcataaaa
     4801 gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct gaataaacgt
     4861 cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg
     4921 cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg
     4981 gcgctgggcg aatgaataac catcagcgcg tcaaaatcct ggctgtggag cagaatatcc
     5041 agcgttttaa tatagtgctc actgctggcg tcatcgcgta gatcgagcgg gttagatatt
     5101 gccacatgtt ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc
     5161 agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg
     5221 ctgataatca tcagccggtc gccatgtagc gggcgcatat ggctaagggt ttccaccgcc
     5281 gaaaacagct cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agccgcatcc
     5341 catgccgggt ccattcctgc cgtggtgttg agcagtcgct gtgccgccgg gctacgtccg
     5401 cttttaatca ccagaatcgg tttattacgc gaggcactac gggccgccga aacaaagcgt
     5461 cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgtgcc
     5521 agatagtcaa gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag
     5581 gaaaagccca tcttacgctg ttgcgcccag tcgaggatgg tgttggagac ggcagccgat
     5641 tgcgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga
     5701 ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat gttatggcgc
     5761 agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt
     5821 ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat
     5881 aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc caacacaccc
     5941 aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc
     6001 aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc aattaccgct
     6061 atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga
     6121 agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac ccagatgttg
     6181 cgttttccct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgctgc
     6241 cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg
     6301 gccttcagcc agggcttcac gcaggcgata ggcagaaagc cgggaaagat cgacggaaat
     6361 gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt ccggccaggt
     6421 accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga tcacttcccg
     6481 ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg ggttttgtac
     6541 cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg
     6601 caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat
     6661 cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt
     6721 ctctggcaaa agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg
     6781 tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gtgttttcgg tcataagaga
     6841 gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa
     6901 gattaaagag attcgttcag ccagctatcg aacggagctt tcggtactgc gccattaagc
     6961 atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag
     7021 cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta
     7081 cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt
     7141 gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag tgtttcaccg
     7201 gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca
     7261 tcttcgatcc gatcgtcggg aatgcgattg atggactgac aatgggtaca aacggtattc
     7321 ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat
     7381 atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc aataggtatg
     7441 atagtcgcag gctttggtac gaaatcatgg agaaccgcgc ggacttcggg taatctgcgc
     7501 gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa
     7561 ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg
     7621 taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc
     7681 ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc cgtggcgcac
     7741 ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg
     7801 taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
     7861 cggcgaaaac gcctgtcagg cactgttcca gagccgtccg gaagcaattg ttcgcgcctg
     7921 gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg
     7981 caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca cggaacatca
     8041 cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag
     8101 ccaggcaggc gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
     8161 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg tgcaggatgc
     8221 ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag agctcgttca
     8281 gtcgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg caggttacac
     8341 cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc tgccagcgaa
     8401 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
     8461 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgtcgc
     8521 aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
     8581 ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg gtaatactgg
     8641 catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg
     8701 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
     8761 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa
     8821 aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag taaacgtctc
     8881 ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca cagtattgag
     8941 cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc gggtgaagcc
     9001 cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa tggcaatgcc
     9061 gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     9121 gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa agacatcttt
     9181 ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     9241 aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta attcgttagc
     9301 catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacagaaca
     9361 aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg gtaaaatttc
     9421 acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat cgatgtgtgg
     9481 tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata cactcaagca
     9541 acaatggttt taccaattag ccgcgacagg ctgaacaaat caaataattt tgccggggag
     9601 gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc
     9661 tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa aagcaggtta
     9721 tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc
     9781 agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct
     9841 gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat
     9901 ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt
     9961 tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag
    10021 agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc
    10081 aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga
    10141 tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg
    10201 acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta
    10261 acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc ccaaaagctg
    10321 attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt ccacgccagc
    10381 cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg gtcaatcact
    10441 gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc cagactcatc
    10501 cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct aagggcgaga
    10561 atattgttcg gtcttggcgt catagcggta atcagggtgt aagtccaaaa agcacttaaa
    10621 agggtcggtg tcacttgtta tctcctctgt t
//
LOCUS       DAJTNN010000140        10648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTNN010000140 DAJTNN010000000
VERSION     DAJTNN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.140"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1247)
                     /gene="icd"
                     /locus_tag="OO150_003156"
     CDS             complement(<1..1247)
                     /gene="icd"
                     /locus_tag="OO150_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5517211.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIEN"
     gene            1419..2072
                     /gene="rluE"
                     /locus_tag="OO150_003157"
     CDS             1419..2072
                     /gene="rluE"
                     /locus_tag="OO150_003157"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5517212.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2082..2543
                     /gene="nudJ"
                     /locus_tag="OO150_003158"
     CDS             2082..2543
                     /gene="nudJ"
                     /locus_tag="OO150_003158"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5517213.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2597..3703
                     /gene="mnmA"
                     /locus_tag="OO150_003159"
     CDS             2597..3703
                     /gene="mnmA"
                     /locus_tag="OO150_003159"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5517214.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3739..4380
                     /gene="hflD"
                     /locus_tag="OO150_003160"
     CDS             3739..4380
                     /gene="hflD"
                     /locus_tag="OO150_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5517215.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4384..5754
                     /gene="purB"
                     /locus_tag="OO150_003161"
     CDS             4384..5754
                     /gene="purB"
                     /locus_tag="OO150_003161"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5517216.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5922..6593
                     /gene="phoP"
                     /locus_tag="OO150_003162"
     CDS             5922..6593
                     /gene="phoP"
                     /locus_tag="OO150_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5517217.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6593..8053
                     /gene="phoQ"
                     /locus_tag="OO150_003163"
     CDS             6593..8053
                     /gene="phoQ"
                     /locus_tag="OO150_003163"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5517218.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8129..9250
                     /gene="roxA"
                     /locus_tag="OO150_003164"
                     /gene_synonym="ycfD"
     CDS             8129..9250
                     /gene="roxA"
                     /locus_tag="OO150_003164"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5517219.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9299..10525)
                     /gene="pepT"
                     /locus_tag="OO150_003165"
     CDS             complement(9299..10525)
                     /gene="pepT"
                     /locus_tag="OO150_003165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5517220.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLAGMKGTVERADMH
                     YIIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
BASE COUNT         2595 a         2746 c         2690 g         2617 t
ORIGIN      
        1 atgttttcga tgatcgcttc accaaactct gaacatttca gcagcttagc gccttccatc
       61 agacgttcaa agtcataagt tacggttttc gcgttgatag caccttccat acctttaaca
      121 attaaatcag ccgcttcagt ccaccccata tggcgcagca tcatctcagc ggagaggatg
      181 atagagcctg gatttacttt gtcctgaccg gcgtatttcg gcgcagtacc gtgggtggct
      241 tcaaacaggg cgcattcgtc accgatgttt gctccaggag cgataccgat accgccaacc
      301 tgcgctgcca gggcgtcaga aatgtagtca ccgttcaggt tcatacaggc gataacatca
      361 tattcagccg gacgcagcag gatctgttgc aggaatgcat cagcaatcac gtctttaatg
      421 acgatctctt tgccggtgtt cgggttttta actttcagcc acgggccgcc gtcgatcagt
      481 tcaccgccaa actcttcacg cgccagctgg tagccccagt ctttaaacgc gccttcggtg
      541 aacttcatga tgttgccttt gtgcaccaga gtcacagagt cacgatcgtt agcaattgcg
      601 tattcgatcg ctgcacgaac cagacgtttg gtgccttctt ccgaacacgg cttaataccg
      661 ataccacaat gttccgggaa gcgaattttc ttcaccccca tctcttcacg caggaattta
      721 atcactttct cggcgtcggc agagtctgct ttccattcga tacccgcata aatgtcttcc
      781 gagttttcac ggaagataac catatcggtc agttcagggt gtttaaccgg gcttggagtg
      841 ccctgatagt aacgtaccgg acgcaggcag atgtagagat ccagttcctg gcgcagggca
      901 acgttcagag agcgaatacc gccaccaaca ggagtggtca gcggaccttt aatggcaacg
      961 cgatattcac gaatcagatc aagggtttca gcaggcagcc agacatcctg accataaacc
     1021 tgtgtggatt tttcaccggt gtaaatttcc atccaggaga ttttacgctc gcctttatag
     1081 gctttctcga ctgcagcgtc gaccactttc agcatggctg gggttacatc tacaccgatt
     1141 ccatcacctt caatgtaagg gataatcgga ttttcaggaa cgttgagttt gccgttttgc
     1201 agggtgatct tcttgccttg tgccggaaca actactttac tttccattca cctctccttc
     1261 gagcgctact ggtttgctcg tctgccacca cgttgcatat gcgtttgcgt cctgcgatac
     1321 ggatgcttta gagcaatttt ttgttaatga tttgtaattg gcttgtcaat actaccctat
     1381 tgtttgccgt catgaaagat gcgcgttatt aggctataat gcggcaattc ataatctctg
     1441 aaaataccat gcaaaaaact tcttttagaa atcacctggt taagcgattc agctcgcaac
     1501 gttctaccag gcgtaaacct gaaaaccagc ccacgcgtgt gatcctgttc aataaaccct
     1561 acgatgttct tccgcagttc accgatgaag ccggacgcaa aacattaaaa gaattcatcc
     1621 cggttcaggg cgtttatgca gcaggtcgtc ttgaccgcga tagcgaaggg ttactggtgc
     1681 tgaccaataa cggcgcgttg caggcgcgtt taacccagcc gggcaaacgc accgggaaaa
     1741 tctattatgt gcaggtggaa ggtattccca cacaagacgc acttgaagcc ttgcgcaatg
     1801 gcgtaacctt aaatgatggc cctaccctgc ccgccggcgc ggagctggtt gacgaaccag
     1861 cgtggttatg gccgcggaat ccaccaattc gtgaacgcaa aagtattccc accagctggc
     1921 tgaagatcac cttatatgaa ggacgtaatc gccaggtgcg ccgcatgacc gcccatgttg
     1981 gtttccccac gctgcgactg attcgttatg cgatgggtga ttacactttg gataaccttg
     2041 ccaatggcga atggcgagaa gcaacagaat aaggatgcac aatgtttaaa ccacacgtta
     2101 ccgttgcttg cgtagtgcac gcagagggta aatttttagt cgttgaagag acgattaatg
     2161 gtaaagcgtt atggaaccaa cctgccgggc atctggaagc cgatgaaacc ttagtggaag
     2221 ctgccgcccg tgagctatgg gaagaaaccg gcatcagcgc gcaaccgcaa cactttattc
     2281 gtatgcatca gtggattgcg ccagataaaa cgccgttttt gcgtttcctc tttgccattg
     2341 agcttgagca aatatgcccg acccagcctc atgacagcga tatcgactgc tgtcgttggg
     2401 tcagcgccga agaaatttta aaggcgtcaa atcttcgttc gccgctggtg gcggaaagta
     2461 tccgttgtta tcaaagcggg caacgctatc cgctggagat gattggcgat tttaactggc
     2521 cttttacaaa gggtgtcatc taaaaggctg cgtgatagaa tacgccgcct tgaagttcaa
     2581 tgtcgtgagt gatccaatgt ctgaaaccgc aaaaaaagta atcgtcggca tgtccggcgg
     2641 tgtcgattcc tccgtttctg cctggctgtt gcaacaacag ggatatcagg ttgaaggcct
     2701 gtttatgaag aactgggaag aagacgacgg tgaggaatat tgcacagcgg cagcggatct
     2761 ggctgatgcc caagctgtct gcgacaagct cggcattgaa ctgcacaccg ttaactttgc
     2821 tgccgagtac tgggacaacg tcttcgaact gttccttgcc gaatataaag ccggtcgcac
     2881 gccgaatccg gatattctgt gcaacaaaga gatcaaattt aaagctttcc tcgaatttgc
     2941 cgccgaagat ttaggtgccg attatatcgc taccggtcat tacgtacgtc gggccgatgt
     3001 cgatggcaag agccgcctgc tgcgtggtct ggacagcaat aaagaccaga gctacttcct
     3061 ttatacgctc agccatgagc agattgcgca aagcctgttc ccggtcggcg aactggaaaa
     3121 accgcaggtg cgtaagattg ctgaagatct tggtctggtc accgcgaaga aaaaagactc
     3181 taccggcatt tgcttcattg gcgaacgtaa attccgcgag ttcctgggcc gttatctccc
     3241 ggcgcaaccg ggcaaaatca ttaccgtcga tggcgatgaa attggtgagc accaggggct
     3301 gatgtatcac actctcggtc agcgtaaagg tctgggtatc ggtggcacca aagaaggcac
     3361 cgaagaaccg tggtatgtgg tggacaaaga cgtcgaaaac aacattctgg ttgttgcgca
     3421 gggccatgaa cacccgcggc tgatgtctgt cgggttgatt gcccagcagt tgcactgggt
     3481 cgatcgcgaa ccattcaccg gtactatgcg ttgcacggta aaaacccgct atcgccagac
     3541 cgatatccct tgcaccgtca aggcgctgga cgatgatcgc attgaagtga ttttcgatga
     3601 accggttgcc gccgtgacgc cgggccagtc tgccgtcttc tataacggtg aagtgtgcct
     3661 cggtggcggt attattgagc agcgtctgcc gctgccggtc tgattattat ctttacttaa
     3721 aacaaggaag cagtgaacgt ggcaaagaat tactatgaca tcaccctcgc cctggccggt
     3781 atttgtcagt cggcacgcct ggtgcaacaa ctcgctcacc aggggcattg tgatgccgat
     3841 gcgctacacg tctcactcaa cagtattatt gatatgaacc ccagctcgac gctggcggtt
     3901 tttggcggta gcgaagccaa cctgcgcgtc gggctggaaa ccctgctcgg cgtgctcaat
     3961 gccagcagtc gccagggctt aaacgccgaa ttaacccgct acacactcag cttgatggtg
     4021 cttgagcgca aactctcctc agcgaaaggc gcgctcgaca ctctgggcaa ccggatcaac
     4081 ggcctgcaac gccagctcga acacttcgat ttacagtccg aaacgctgat gagcgcgatg
     4141 gctgctatct atgttgatgt gatcagcccg cttggcccgc gcattcaggt caccggttcc
     4201 cctgctgtac tgcaaagccc acaagtgccg gcgaaagttc gcgcaaccct gctggcaggc
     4261 attcgcgccg ccgtgctctg gcaccaggtc ggcggcggac gtctgcaact gatgttttct
     4321 cgtaatcgcc tgaccactca ggcaaaacaa attcttgctc atttaacccc ggagttgtga
     4381 tctatggaat tatcctcact gaccgccgtt tcccctgtcg atggacgcta cggcgataaa
     4441 gtcagcgcgc tgcgcgggat tttcagcgaa tatggtttgc tgaaattccg tgtacaagtt
     4501 gaagtacgtt ggctgcaaaa actggccgcg cacgcagcga tcaaggaagt tcctgctttt
     4561 gctgccgacg caatcggtta ccttgatgca attgtcgcca atttcagcga agaagatgcc
     4621 gcacgcatca aaaccatcga gcgtaccact aaccacgacg ttaaagcggt tgagtatttc
     4681 ctgaaagaaa aagtggcgga gatcccggaa ctgcacgcgg tttctgaatt catccacttt
     4741 gcctgtactt cggaagatat caataacctc tcccacgcat taatgctgaa aaccgcgcgt
     4801 gatgaagtga tcctgccgta ctggcgtcaa ctgattgatg gcattaaaga tctcgccgtt
     4861 cagtaccgcg atatcccgct gctgtctcgt acccacggtc agccagccac gccgtcaacc
     4921 atcggtaaag agatggcaaa cgtcgcctac cgtatggagc gccagtaccg ccagcttaac
     4981 caggtggaga tcctcggcaa aatcaacggc gcggtcggta actataacgc ccacatcgcc
     5041 gcttacccgg aagttgactg gcatcagttc agcgaagagt tcgtcacctc gctgggtatt
     5101 cagtggaacc cgtacactac ccagattgaa ccgcacgact acattgccga actgtttgat
     5161 tgcgttgcgc gcttcaacac cattctgatc gactttgacc gtgacgtctg gggttatatc
     5221 gcccttaacc acttcaaaca gaaaaccatt gctggtgaga ttggttcttc caccatgccg
     5281 cataaagtta acccgatcga cttcgagaac tccgaaggga atctgggcct ttccaacgcg
     5341 gtattacagc atctggcaag caaactgccg gtttcccgct ggcagcgtga cctgaccgac
     5401 tccaccgtgc tgcgtaacct cggcgtgggt atcggttatg cgctgattgc gtatcaatcc
     5461 accctgaaag gcgtgagcaa actggaagtg aaccgtgacc atctgctgga tgagctggat
     5521 cacaactggg aagtgctggc tgagccaatc cagacagtta tgcgtcgcta tggcatcgaa
     5581 aaaccgtacg agaagctgaa agagctgact cgtggtaagc gcgttgacgc cgaaggcatg
     5641 aagcagttta tcgacggtct ggcgttgcca gaagaagaga aagcccgcct gaaagcgatg
     5701 acgccggcaa actacattgg tcgcgccatc accatggttg atgagctgaa ataaactcgt
     5761 atcagtgccg gatggcgatg ctgtccggcc tgcttattaa gattatccgc tttttatttt
     5821 ttcactttac ctcccctccc cgctggttta tttaatgttt acccccataa ccacataatc
     5881 gcgttacact attttaataa ttaagacagg gagaaataaa aatgcgcgta ctggttgttg
     5941 aagacaatgc gttgttacgt caccacctta aagttcagat tcaggatgct ggtcatcagg
     6001 tcgatgatgc agaagatgcc aaagaagccg attattatct caatgaacat ttaccggata
     6061 ttgcgattgt cgatctcgga ttgccagacg aggacggtct gtcactgatt cgccgctggc
     6121 gtagcaatga tgtttcactg ccgattctgg tattaaccgc ccgtgaaagc tggcaggaca
     6181 aagtcgaagt attaagtgct ggtgctgatg attacgtgac taaaccattt catattgaag
     6241 aggtgatggc gcgaatgcag gcattaatgc ggcgtaatag cggtctggct tcacaggtca
     6301 tttcgctacc tccgtttcag gttgatctct ctcgccgtga attatctatt aatgacgaag
     6361 tgatcaaact gaccgcgttc gaatacacca ttatggaaac gttgatacgc aataatggca
     6421 aagtggtgag caaagattcg ttaatgctcc aactctatcc ggatgcggag ctgcgggaaa
     6481 gccataccat tgatgtactg atgggacgtc tgcgcaaaaa aattcaggca caatatccgc
     6541 aagaagtgat tactaccgtt cgcggccagg gctatttgtt cgaattgcgc tgatgaaaaa
     6601 attactgcgt ctttttttcc cgctctcgct gcgggtacgt tttctgttgg caacggctgc
     6661 ggtagtactg gtgctttcgc ttgcctacgg aatggtcgcg ctgatcggtt atagcgtcag
     6721 tttcgataaa actacgtttc ggctgttacg tggcgagagc aatctgttct atacccttgc
     6781 gaagtgggaa aacaataagt tgcatgtcga gttacccgaa aatatcgaca agcaaagccc
     6841 caccatgacg ctaatttatg atgagaacgg gcagctttta tgggcgcaac gtgacgtgcc
     6901 ctggctgatg aagatgatcc agcctgactg gctgaaatcg aatggttttc atgaaattga
     6961 agcggatgtt aacgatacca gcctcttgct gagtggagat cattcgatac agcaacagtt
     7021 gcaggaagtg cgggaagatg atgacgacgc ggagatgacc cactcggtgg cagtaaacgt
     7081 ctacccggca acatcgcgga tgccaaaatt aaccattgtg gtggtggata ccattccggt
     7141 ggagctaaaa agttcctata tggtctggag ctggtttatc tatgtgctct cagccaatct
     7201 gctgttagtg atcccgctgc tgtgggtcgc cgcctggtgg agtttacgcc ccatcgaagc
     7261 cctggcaaaa gaagtccgcg aactggaaga acataaccgc gaattgctca atccagccac
     7321 aacgcgagaa ctgaccagtc tggtacgaaa cctgaaccga ttgttaaaaa gtgaacgcga
     7381 acgttacgac aaataccgta cgacgctcac cgacctgacc catagtctga aaacgccact
     7441 ggcggtgctg caaagtacgc tgcgttctct gcgtagtgaa aagatgagcg tcagtgatgc
     7501 tgagccggta atgctggagc aaatcagccg catttcacag caaattggct actacctgca
     7561 tcgtgccagt atgcgcggcg ggacattgct cagccgcgag ctgcatccgg tcgccccact
     7621 gctggacaat ctcacctcag cgctgaacaa agtgtatcaa cgcaaagggg tcaatatctc
     7681 tctcgatatt tcgccagaga tcagctttgt cggtgagcag aacgattttg tcgaggtgat
     7741 gggcaacgtg ctggataatg cctgtaaata ttgcctcgag tttgtcgaaa tttctgcaag
     7801 gcaaaccgac gagcatctct atattgtggt cgaggatgat ggccccggta ttccattaag
     7861 caagcgagag gtcattttcg accgtggtca acgggttgat actttacgcc ctgggcaagg
     7921 tgtagggctg gcggtagccc gcgaaatcac cgagcaatat gagggtaaaa tcgtcgccgg
     7981 agagagcatg ctgggcggtg cgcggatgga ggtgattttt ggtcgccagc attctgcgcc
     8041 gaaagatgaa taaatatgtc catacttcac gcattacgtt aagcatccgt tataatcggt
     8101 tgcagatacc agcctgtgga tgcttaacat ggaataccaa ctcactctta actggcccga
     8161 ttttcttgaa cgtcactggc agaaacgccc ggtggtgtta aaacgcggct ttaataattt
     8221 tattgacccg atctctccag acgagttggc gggtctggcg atggaaagcg aagttgacag
     8281 tcgactggtc agtcaccagg atggcaaatg gcaggtcagc cacggcccgt tcgaaagcta
     8341 cgatcatctc ggtgaaacca actggtcatt actggtacag gcagtgaacc actggcatga
     8401 gccgaccgcc gcgctgatgc gaccgttccg tgaactaccg gactggcgta ttgatgatct
     8461 gatgatttct ttttctgtac ctggcggcgg cgtcggcccg catctcgatc agtacgacgt
     8521 gtttatcatt cagggtaccg gacgtcgtcg ctggcgagtg ggcgaaaagc tgcaaatgaa
     8581 acagcactgc ccacatccgg atctgttaca ggtcgatccg ttcgaagcca tcatcgatga
     8641 agagctggag cctggtgata ttctttatat tccgccagga ttcccgcatg aaggctacgc
     8701 gctggaaaat gcgatgaact attccgtggg ctttcgcgcg ccaaatacgc gggaactgat
     8761 tagtggattt gccgattatg tgctgcaacg tgaactgggc ggcaactact acagcgatcc
     8821 tgatgttcca cctcgcgctc atcctgcgga tgttctgcct caagagatgg ataaactgcg
     8881 tgagatgatg ctcgaattga tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt
     8941 tatatcccag tcacgtcatg aactggatat cgcgccgccg gaaccgcctt atcagccaga
     9001 tgaaatctac gatgcgctga aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt
     9061 attgcgcatt ggcgacgacg tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc
     9121 ggcactggat gcactcgcca gcaacattgc gctgactgcg gagaattttg gcgatgcgct
     9181 ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc aatagcgggt attggttctt
     9241 cgaagggtaa gtttggattt aagccggatg cggcatcaca tgccgcatcc cttttcgctt
     9301 acttccgttg cgccgttaac tcggcaatac ggacgatcac ctgcaccgct ttttccatac
     9361 cttccagagt cacaaactca tgcttaccat gatagttgta accgccagtg aacaagttcg
     9421 ggcacggtaa tcccataaac gacaactgcg cgccgtcggt accgccgcgg atcggtttca
     9481 gttccggttc aatatcgcag tcacgcattg cctgctgggc gatatcgaga atatgcggat
     9541 gctcaaccac tttctcgcgc atattgtagt aactgtcttc aatcaccagt tcaatgtagc
     9601 aatcaggatg taaccctttg cccacttttt tggcgatctc catcatttta catttacgcg
     9661 cttcaaactg tttacggtcg aaatcacgga tgatgtagtg catatcggcc cgttcaacgg
     9721 tgcctttcat gcccgccagg tgatagaaac cttcatagcc ttctgtcatt tccgggcttt
     9781 catccgccgg aacttccgca tgaatacgtg ccgccagcga cagcgcattt accatcactc
     9841 ctttcgccgt gcccggatga acattgttac cgacaatttt gatattgacc gatgcggcgt
     9901 tgaagttttc aaactccagt tcgcctacgc caccaccgtc aacagtgtaa gcccagcggg
     9961 catcgaaggc atcaacatca aaatgtttcg cccctttgcc cacttcttca tccggggtaa
    10021 aggcgacgcg aatatcacca tgcggaatgt ttttctgttg caataccgcc agcgcggtca
    10081 tgatttctgc aatacctgct ttgtcatcgg cacctaacaa ggttttacca tcggtggtaa
    10141 tcaatgtctg acccagtagc tgatgcagca ccgggaacat aaccggtgat aaaacttcat
    10201 cgccgatacc cagcgcaata tcgccaccgc gatagttttc aacaatttgc ggattaacat
    10261 ttttgccgct gcaatccggt gaggtatcca catgagaaat aaagccaatc gccgggatat
    10321 cgccagggac gttagccggt aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat
    10381 tgataagccc catttcttcg agctgctctt tcagcagatg caataacttc cattggcctt
    10441 ccgtgctggg aacctgtctc acccctgctt ttgattgggt atccagagac acgtagttca
    10501 aaaatcgctc aagtagttta tccatgtagt caccctcact ttttgtgaca acattattaa
    10561 gaagcaagaa aagacaaata ttgcgtcagg tcacttttac ccctgcaagc gggaatattt
    10621 atcagcatta ccttaatgaa tataaagc
//
LOCUS       DAJTNN010000141        10410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTNN010000141 DAJTNN010000000
VERSION     DAJTNN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.141"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(4..1902)
                     /gene="espL1"
                     /locus_tag="OO150_003166"
     CDS             complement(4..1902)
                     /gene="espL1"
                     /locus_tag="OO150_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR5517221.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSELINQFARQLITLLD
                     TSDLSSMLSYCITQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAC
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQLVYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPVGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLIYNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDETIDIFLNALTTPIAQELLNKKIVMDILAMKTRDGELGLFAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAHGTPVLYIAMSKGNEDVVLSYISTL
                     NIFAKKYSFSQRQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     ADELKTYL"
     gene            2424..4352
                     /gene="thrS"
                     /locus_tag="OO150_003167"
     CDS             2424..4352
                     /gene="thrS"
                     /locus_tag="OO150_003167"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5517222.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4356..4898
                     /gene="infC"
                     /locus_tag="OO150_003168"
     CDS             4356..4898
                     /gene="infC"
                     /locus_tag="OO150_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5517223.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQRVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4995..5192
                     /gene="rpmI"
                     /locus_tag="OO150_003169"
     CDS             4995..5192
                     /gene="rpmI"
                     /locus_tag="OO150_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5517224.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5245..5601
                     /gene="rplT"
                     /locus_tag="OO150_003170"
     CDS             5245..5601
                     /gene="rplT"
                     /locus_tag="OO150_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5517225.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5718..5842
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5724..5768
                     /gene="pheM"
                     /locus_tag="OO150_003171"
     CDS             5724..5768
                     /gene="pheM"
                     /locus_tag="OO150_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5517226.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6051..7034
                     /gene="pheS"
                     /locus_tag="OO150_003172"
     CDS             6051..7034
                     /gene="pheS"
                     /locus_tag="OO150_003172"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5517227.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7049..9436
                     /gene="pheT"
                     /locus_tag="OO150_003173"
     CDS             7049..9436
                     /gene="pheT"
                     /locus_tag="OO150_003173"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5517228.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9441..9740
                     /gene="ihfA"
                     /locus_tag="OO150_003174"
     CDS             9441..9740
                     /gene="ihfA"
                     /locus_tag="OO150_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5517229.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(10044..10184)
                     /locus_tag="OO150_003175"
     CDS             complement(10044..10184)
                     /locus_tag="OO150_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR5517230.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2736 a         2357 c         2655 g         2662 t
ORIGIN      
        1 tggttataaa tacgtcttta gttcatcagc actaaaactc aggctttggc tgattacatt
       61 tatagcagca taatatgttt ctacagtttt ataatgatta tgatgaatgg ctatatgaac
      121 agctgacatg ttgtcatgat ttttagcggc caacagtgtg aataactgac gttgactaaa
      181 agaatatttt tttgcaaaaa tattcagcgt cgatatataa gataacacga cgtcttcatt
      241 acccttgctc atggcgatgt ataatacagg ggttccatgt gctgtagcgc cttttaataa
      301 atccatgatg ttagctttgc tcaacttgta tttaaaggcg atgccattaa ttttagagag
      361 gaaccgtgtg acacacaaag ggtgattatt ttccattgca gcaaacaacc ctagttcacc
      421 atcacgtgtt ttcattgcta aaatatccat cactattttt ttgtttaaaa gctcttgtgc
      481 tattggagtg gttaatgcat tcagaaagat atcgattgtt tcatcgaacc catacaacat
      541 ggagatatag agcccaggtg tttcgttagc acttttagtt tgcagtaatt taaccagatt
      601 atcttcatga atgatatgat tgtatatcag tgactgaatt tcttgcataa atatttttac
      661 gttgtcagta tgtccgttca tcatagccag aaataaagca ggtgtaccaa cgctattctg
      721 cgcagttatt atttcaatga tttctttttc agggagtatg ggcaactgtt tgaataatgt
      781 aataagctgt tgagtctggc ctgtggccat tattaacaac attgccgatg gatgtatagg
      841 ggcaaatggc attcgtgatg caatacccgc gaaaatagcc cctggaactc ccacaggtaa
      901 taggggcaga atagcaattg cacagttgcg aacataccta tcagcccagg tctgagcatc
      961 gggacctgaa ctgatgaact tatcaagcga aagaaagccc cgtgctgtcc tgtgggtgcc
     1021 tctgacggca acgttggtat cgttcgggtc gtagacggac actacatagt ttaattgtcc
     1081 atacactaac tgctttattc gtagccttac tgacaaagta tgattgtcta tatataaaat
     1141 cgcggacata tgactgacat tgtttgccat cattcgacaa aagaaaatat ctaaagcgaa
     1201 gccaaaatcc ttactcgcga taacgcgact ccctgcagga ggatggcgaa gtaatgctct
     1261 gaactccgca tcccggacta aggggacatg ctgctgtatg gattcaacgg aggagtaatg
     1321 tttaatcagg ttgactttgc cttttccatt tctcaatgaa tcaagggcgt attgcaatga
     1381 cagatggcga caaaaaatag cgtcagaagg agattgcaaa aaacatgcac taccattgag
     1441 atttactgtt ggttgtatag ttttttctct atggattttt ttagacagtt tcatacaatg
     1501 gttacgatct tcggcagtta actgttcctg ctcaatataa tccagtatgc ttcgcaaatg
     1561 gttagcctta ataggcgtgg tttccagaaa taagggaatg atttcttttt tatagtgtaa
     1621 tgtaataagt atattttctg catggatggg tgaagtggca aagttaatga taaaaagggc
     1681 agtggagtaa caattttgtg atatttttcg acagtttgcg gtaaattcct gagtaataca
     1741 gtatgacagc atcgaactta agtcagaggt atcaagtagg gtgatgagtt ggcgcgcaaa
     1801 ttgattaata agctctgatg aaggcgttgg tatgtcgcta tcgatgtgca atctatgagg
     1861 agacttataa tgagttctga taatgatatc gttttgactc ataatctatt caccttatat
     1921 atactggagg ttttgaggat gatgattaat tattatcgcc agaacgaaat gtgcacgaaa
     1981 aattgctata agaattgtgt cacttttatc attgtgatat gaccacttcg tcattaatct
     2041 catgatcgtt taaatactac cgccaaatca cgcaacgaac tttgctggat tcagtttatg
     2101 ctgctgtaaa tccgctcgca gtaaaccttt cagacgcacc gtgatgttat cagtggttct
     2161 ttaagcgttt tgctggtgta ctcactacaa acgaattgcg aatcgaatca atgtgaaacg
     2221 gaaaggtaca atctcccttt ttttagtgtt aacatcgttc aaccgggtgg aaggtgagag
     2281 gttaatgtct ttcatttgtg tacctgattg cgaaccaatt tagcatttgt tggctagatg
     2341 gtttcgcaat gaactgttaa taaacaaatt tttctttgta tgtgatcttt cgtgtgggtc
     2401 accactgcaa ataaggatat aaaatgcctg ttataactct tcctgatggc agccaacgcc
     2461 attacgacca cgctgtaagc cccatggatg ttgcgctgga cattggtcca ggtctggcga
     2521 aagcctgtat cgcagggcgc gttaatggcg aactggttga tgcttgcgat ctgattgaaa
     2581 acgacgcaca actgtcgatc attaccgcca aagacgaaga aggtctggag atcattcgtc
     2641 actcctgtgc gcacctgtta gggcatgcga ttaaacaact ttggccgcat accaaaatgg
     2701 caatcggccc ggttattgac aacggttttt attacgacgt tgatcttgac cgcacgttaa
     2761 cccaggaaga tgtcgaagca ctcgagaagc ggatgcatga gcttgctgag aaaaactacg
     2821 acgtcattaa gaagaaagtc agctggcacg cagcgcgtga aactttcgcc aaccgtgggg
     2881 agagctacaa agtctccatt cttgacgaaa acatcgccca tgatgacaag ccaggtctgt
     2941 acttccatga agaatatgtc gatatgtgcc gcggtccgca cgtaccgaac atgcgtttct
     3001 gccatcattt caaactaatg aaaacggcag gggcttactg gcgtggcgac agcaacaaca
     3061 aaatgttgca acgtatttac ggtacggcgt gggcagacaa aaaagcactt aacgcttacc
     3121 tgcagcgcct ggaagaagcc gcgaaacgcg accaccgtaa aatcggtaaa cagctcgacc
     3181 tgtaccatat gcaggaagaa gcgccgggta tggtattctg gcacaacgac ggctggacca
     3241 tcttccgtga actggaagtg tttgttcgtt ctaaactgaa agagtaccag tatcaggaag
     3301 ttaaaggtcc gttcatgatg gaccgtgtcc tgtgggaaaa aaccggtcac tgggacaact
     3361 acaaagatgc aatgttcacc acatcttctg agaaccgtga atactgcatt aagccgatga
     3421 actgcccggg tcacgtacaa attttcaacc aggggctgaa gtcttatcgc gatctgccgc
     3481 tgcgtatggc cgagtttggt agctgccacc gtaacgagcc gtcaggttcg ctgcatggcc
     3541 tgatgcgcgt gcgtggattt acccaagatg acgcgcatat cttctgtact gaagaacaaa
     3601 ttcgcgatga agttaacgga tgtatccgtt tagtctatga tatgtacagc acttttggct
     3661 tcgagaagat cgtcgtcaaa ctctccactc gtcctgaaaa acgtattggc agcgacgaaa
     3721 tgtgggatcg tgctgaggcg gacctggcgg ttgcgctgga agaaaacaac atcccgtttg
     3781 aatatcaact gggtgaaggc gctttctacg gtccgaaaat tgaatttacc ctgtatgact
     3841 gcctcgatcg tgcatggcag tgcggtacag tacagctgga cttctccttg ccgtctcgtc
     3901 tgagcgcctc ctatgtgggc gaagacaacg agcgtaaggt accggtaatg attcaccgcg
     3961 caattctggg gtcgatggaa cgtttcatcg gtatcctgac cgaagaattc gctggtttct
     4021 tcccgacctg gcttgcgcca gttcaggttg ttatcatgaa tattaccgat tcacagtctg
     4081 aatacgttaa cgaattgacg caaaaactat caaatgcggg cattcgtgtt aaagcagact
     4141 tgagaaatga gaagattggc tttaaaatcc gcgagcacac tttgcgtcgc gtcccatata
     4201 tgctggtctg tggtgataaa gaggtggaat caggcaaagt tgccgttcgc acccgccgtg
     4261 gtaaagacct gggaagcatg gacgtaaatg aagtgatcga gaagttgcaa caagagattc
     4321 gcagccgcag tcttaaacaa ttggaggaat aaggtattaa aggcggaaaa cgagttcaaa
     4381 cggcgcgccc taaccgtatc aatggcgaaa ttcgcgccca ggaagttcgc ttaacaggtc
     4441 tggaaggcga gcagcttggt attgtgagtc tgagagaagc tctggagaaa gcagaagaag
     4501 ccggagtaga cttagtcgag atcagcccta acgccgagcc gccggtttgt cgtataatgg
     4561 attacggcaa attcctctat gaaaagagca agtcttctaa ggaacagaag aaaaagcaaa
     4621 gagttatcca ggttaaggaa attaaattcc gtcctggtac agatgaaggc gactatcagg
     4681 taaaactccg cagcctgatt cgctttctcg aagagggtga taaagccaaa atcacgctgc
     4741 gtttccgcgg tcgtgagatg gcgcaccagc aaatcggtat ggaagtgctt aatcgcgtga
     4801 aagacgattt gcaagaactg gcagtggtcg aatccttccc aacgaagatc gaaggccgcc
     4861 agatgatcat ggtgctcgct cctaagaaga aacagtaagg ccttcaagta gcactgtctg
     4921 tggagccttc gggcttcaca ggctttgttc gcctttgttt cgtttattaa caatgcgaag
     4981 tggaagttat taaaatgcca aaaattaaga ccgtacgcgg tgctgctaag cgcttcaaaa
     5041 aaaccggtaa aggtggtttt aagcacaagc acgctaacct gcgtcacatt ctgaccaaaa
     5101 aagcgaccaa acgtaaacgt cacctgcgtc cgaaagccat ggtttccaaa ggcgatctgg
     5161 gcctggtaat cgcgtgcctg ccgtacgcat aagccgttaa cgtttttaac tttttaatta
     5221 gaatatagat acaggagagc acatatggct cgcgtaaaac gtggtgttat tgcacgcgca
     5281 cgtcacaaga aaattttgaa acaagctaaa ggctactacg gtgcgcgttc tcgcgtatac
     5341 cgcgttgcct tccaggctgt tatcaaagct ggtcagtatg cttaccgtga ccgtcgtcaa
     5401 cgtaagcgtc agttccgtca actgtggatt gcacgtatca acgcagcagc acgtcagaac
     5461 ggtatttctt acagcaaatt catcaatggc ctgaaaaaag cctctgttga aatcgaccgt
     5521 aagatcctgg ctgatatcgc agtattcgac aaagtagcgt tcaccgctct ggttgaaaaa
     5581 gcgaaagcag ctctggcata agccagttga aagagggagc ttgtctccct cttttcgttt
     5641 caacgccatc aaaacattga cttttatcgc cgtagccttt tcaataaagg tcttttgaag
     5701 agtaccaaaa ggtaacgcaa gcaatgaatg ctgctatttt ccgcttcttt ttttacttta
     5761 gcacctgaat ccaggaggct agcgcgtgag aagagaaacg gaaaacagcg cctgaaagcc
     5821 tcccagtgga ggcttttttt gtatgcgcgt tttgaaaaat aaaaagccag tctatcaggc
     5881 gtaattgtcg cagccagtct ggatacggcc agcgcggaga aaccggagcg tatttaagta
     5941 cgtgagaatt tcgagcactg cccgggacca aaatggcaag taaaatagcc tgatgggata
     6001 ggctctaagt ccaacgaacc agtgtcacca ctgacacaat gaggaaaacc atgtcacatc
     6061 tcgcagaact ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt
     6121 tagataatgt gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt cagatgacga
     6181 ccctgcgtga gctgccgcca gaagagcgtc cggcagctgg tgcggttatc aacgaagcga
     6241 aagagcaggt tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc gcagcgctga
     6301 atgcgcgtct ggcggcggaa acgattgatg tctccctgcc aggtcgtcgc attgaaaacg
     6361 gcggtctgca tccggttacc cgtaccatcg accgtatcga aagtttcttc ggtgagcttg
     6421 gctttaccgt ggcaaccggg ccggaaatcg aagacgatta tcataacttc gatgctctga
     6481 acattcctgg tcaccacccg gcgcgcgctg accacgacac tttctggttt gacactaccc
     6541 gcctgctgcg tacccagacc tctggcgtac agatccgcac catgaaagcc cagcagccac
     6601 cgattcgtat catcgcgcct ggccgcgttt atcgtaacga ctacgaccag actcacacgc
     6661 cgatgttcca tcagatggaa ggtctgattg ttgataccaa catcagcttt accaacctga
     6721 aaggcactct gcacgatttc ctgcgtaact tctttgagga agatttgcag attcgcttcc
     6781 gtccttccta cttcccgttc accgagcctt ctgcagaagt tgacgtcatg ggtaaaaacg
     6841 gtaaatggct ggaagtgctg ggttgcggga tggtgcatcc gaacgtgctg cgtaacgttg
     6901 gcatcgaccc ggaagtttac tctggtttcg ccttcgggat ggggatggag cgtctgacta
     6961 tgttgcgtta cggcgtcacc gacctgcgtt cattcttcga aaacgatctg cgtttcctca
     7021 aacagtttaa ataaggcagg aatagattat gaaattcagt gaactgtggt tacgcgaatg
     7081 ggtgaacccg gcgattgata gcgatgcgct ggcgaatcaa atcactatgg cgggcctgga
     7141 agttgacggt gtagaaccgg ttgccggtag cttccacggc gtggtcgttg gtgaagtggt
     7201 tgagtgtgcg cagcatccga acgctgacaa actgcgtgtg acaaaagtga atgtcggcgg
     7261 cgatcgcctg ctggacatcg tctgtggtgc gccaaactgc cgtcagggcc tgcgtgtggc
     7321 ggtagctacc attggtgctg ttctgccggg tgatttcaaa attaaagcgg cgaaactgcg
     7381 tggcgaaccg tctgaaggga tgctgtgctc cttctctgaa ctgggaattt ctgacgatca
     7441 cagcggcatt atcgaactgc ctgcagatgc gccgattggc actgacatcc gcgaatacct
     7501 gaaactcgat gacaacacca tcgaaatcag cgtaacgcca aaccgtgccg actgcttagg
     7561 tatcattggt gttgcgcgtg acgttgccgt gctgaatcag ctgccgctgg ttgaaccgga
     7621 aatcgttccg gttggtgcga ccatcgacga cacgttgccg attacagtcg aagcgcctga
     7681 agcctgcccg cgctatcttg gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc
     7741 gctgtggatg aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg cagttgttga
     7801 cgtcaccaac tatgtactgc tcgaactggg ccagccgatg cacgctttcg ataaagatcg
     7861 cattgaaggc ggcattgtgg tgcggatggc aaaagagggc gaaacgctgg tgctgcttga
     7921 tggcactgaa gcgaagctga atgctgacac tctggtcatc gccgaccaca acaaggcgct
     7981 ggcgatgggc ggcatctttg gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt
     8041 cttgctggaa tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg
     8101 cctgcatact gatgcgtctc accgttatga gcgtggcgtt gatccggcac tgcagcacaa
     8161 agcgatggaa cgtgcgaccc gtctgctgat cgacatctgc ggtggtgagg ctggtccggt
     8221 aattgatatc accaacgaag caacgctgcc gaagcgtgca accatcactt tacgtcgtag
     8281 caaactggat tgcctgatcg gccatcatat tgctgatgaa caggtaactg acattctgcg
     8341 tcgtctcggc tgcgaagtga ccgaaggcaa agacgagtgg caggcagttg cgccgagctg
     8401 gcgtttcgat atggagattg aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta
     8461 caacaacatc ccggatgagc cggtacaggc aagcctgatt atgggtactc accgtgaagc
     8521 tgacctgtcg ctcaagcgcg tgaaaacgct gctcaacgac aaaggctatc aggaagtgat
     8581 cacctacagc ttcgttgatc cgaaagtgca gcagatgatc catccaggcg ttgaagcctt
     8641 actgctgcca agcccgatct ctgttgaaat gtcagcaatg cgtctttctc tgtggaccgg
     8701 cctgctggca accgtggtgt acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga
     8761 aagcggtctg cgtttcgtac cagatactca ggcaccgttg ggcattcgtc aggatctgat
     8821 gttagccggt gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg caaaagagac
     8881 cgttgatttc tatgatttga aaggcgatct tgaatccgtt ctcgacctga ccggtaaact
     8941 gaatgaggtt gagttccgtg cagaagcgaa tccggcactg catccggggc aatccgcagc
     9001 gatttatctg aaaggggaac gtattggttt tgttggagtt gttcatcctg aactggaacg
     9061 taaactggat cttaacggtc gcactctggt gttcgaactg gagtggaaca agctcgcaga
     9121 ccgcgtggtg cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc
     9181 ggtggtggtc gcagaaaacg ttcccgcagc ggatatttta tccgaatgta agaaagttgg
     9241 cgtaaatcag gtagttggcg taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga
     9301 ggggtataag agcctcgcca taagcctgat cctgcaagat accagccgta cactcgaaga
     9361 agaggagatt gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc gattccaggc
     9421 atcattgagg gattgaacct atggcgctta caaaagctga aatgtcagaa tatctgtttg
     9481 ataagcttgg gcttagcaag cgggatgcca aagaactggt tgaactgttt ttcgaagaga
     9541 tccgtcgcgc tctggaaaac ggcgaacagg tgaaactctc tggttttggt aacttcgatc
     9601 tgcgtgataa gaatcaacgc ccgggacgta acccgaaaac gggcgaggat attcccatta
     9661 cagcacggcg cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg
     9721 cttcgcccaa agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct
     9781 ttcaataact aattgattta taatgtttta tatctcttcg ttccacgatg cgtccacatt
     9841 tagtgggggg aggggagtaa gcccgctaca ttgtgggctt tgtcttatag tctgatcact
     9901 gtttcaagtc ggtgaggaca ccaaaaagaa cccccgcagt ttttacgctg cgggtttgtt
     9961 gttcatgtct gtgagatagg gtgccttatc gaccttaccc tggcaaccga ttgacggggg
    10021 attgctcccc cgtcgcggtt tccttactgc ttacactgta agaacgccgc aaactccgct
    10081 ccccagaagc tcatccgtat ttcgcacagc gaaccgtgca gcatccagat gatgaggatt
    10141 gccgtcacgc agaacgtgat ggccgtaagc gatttttgcg acatagcgct tgctcctttt
    10201 tcggggaggc gctaacctat cacttgctta gggtagacgg ttagggcctc ggttaaacaa
    10261 aaatgttttc cggggccttt ccacatctgg ccttcaggta ttccctccga ccatcagccg
    10321 aaaggcaccc gcgcgtaatc tatcgctttt ttgttactcc ggcaattctg ctcgttaatt
    10381 ctgaggtaag ggcaaactca tctgattgtt
//
LOCUS       DAJTNN010000142        10294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTNN010000142 DAJTNN010000000
VERSION     DAJTNN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.142"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..401
                     /locus_tag="OO150_003176"
                     /pseudo
     CDS             1..401
                     /locus_tag="OO150_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="OO150_003177"
     CDS             484..741
                     /locus_tag="OO150_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517231.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO150_003178"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO150_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5517232.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO150_003179"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO150_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5517233.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO150_003180"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO150_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5517234.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO150_003181"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO150_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5517235.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO150_003182"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO150_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5517236.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO150_003183"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO150_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5517237.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO150_003184"
     CDS             complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO150_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5517238.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9142..9324
                     /locus_tag="OO150_003185"
                     /pseudo
     CDS             9142..9324
                     /locus_tag="OO150_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9318..9422
                     /locus_tag="OO150_003186"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="OO150_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="OO150_003187"
     CDS             9762..10019
                     /locus_tag="OO150_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5517239.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2401 a         2734 c         2822 g         2337 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaatgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agca
//
LOCUS       DAJTNN010000143        10288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTNN010000143 DAJTNN010000000
VERSION     DAJTNN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.143"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            672..1037
                     /locus_tag="OO150_003188"
     CDS             672..1037
                     /locus_tag="OO150_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5517240.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1077..1781
                     /locus_tag="OO150_003189"
     CDS             1077..1781
                     /locus_tag="OO150_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR5517241.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGE
                     NEGVDHKVIDAFSPAG"
     gene            1848..3266
                     /gene="dcm"
                     /locus_tag="OO150_003190"
     CDS             1848..3266
                     /gene="dcm"
                     /locus_tag="OO150_003190"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5517242.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3247..3717
                     /gene="vsr"
                     /locus_tag="OO150_003191"
     CDS             3247..3717
                     /gene="vsr"
                     /locus_tag="OO150_003191"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5517243.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3706..4626)
                     /gene="yedA"
                     /locus_tag="OO150_003192"
     CDS             complement(3706..4626)
                     /gene="yedA"
                     /locus_tag="OO150_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5517244.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4799..5716
                     /locus_tag="OO150_003193"
     CDS             4799..5716
                     /locus_tag="OO150_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5517245.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5795..5977
                     /locus_tag="OO150_003194"
     CDS             5795..5977
                     /locus_tag="OO150_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5517246.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6148..7842
                     /gene="yedQ"
                     /locus_tag="OO150_003195"
                     /pseudo
     CDS             6148..7842
                     /gene="yedQ"
                     /locus_tag="OO150_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(7839..8654)
                     /locus_tag="OO150_003196"
                     /pseudo
     CDS             complement(7839..8654)
                     /locus_tag="OO150_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(8952..9168)
                     /gene="yodD"
                     /locus_tag="OO150_003197"
                     /pseudo
     CDS             complement(8952..9168)
                     /gene="yodD"
                     /locus_tag="OO150_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            9331..9519
                     /gene="dsrB"
                     /locus_tag="OO150_003198"
     CDS             9331..9519
                     /gene="dsrB"
                     /locus_tag="OO150_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5517247.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9563..10186)
                     /gene="rcsA"
                     /locus_tag="OO150_003199"
     CDS             complement(9563..10186)
                     /gene="rcsA"
                     /locus_tag="OO150_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5517248.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
BASE COUNT         2626 a         2429 c         2670 g         2563 t
ORIGIN      
        1 cttgccgtag aaatcaactt tattgccatc tttattgtaa atttcagccg catttgctgc
       61 gccagcaact aataacgccg ggaccagcat tgccagaact tttcttttca ttatgtattc
      121 ccttgtgatt ataatcttca tgaatatatc aataagtgcc gttatccaaa aaaaagcaca
      181 tttggatact attctatgaa gttcatttca ttttaagtat tacatgcaac aaatgtattt
      241 aaaagatttc aaaatgtttc taatctattt aatataattc agagatatga attataaata
      301 ttcatgaaaa ttataaatgt atatcaatat gttatgagtt gagcggaaac atagggggta
      361 tcaatgattg ttgcgccaaa cggctggtgt gatcaggcgc acattaatga aggcataaaa
      421 aaaagccggc atcatgccgg cccaaaaaat ataaacaaga cgataaaagg ctaataacgg
      481 aagcatcatg acacagaata ataaaaaggt ataattgtaa tcagtgctaa tatattgatt
      541 aaatgaatta ataaagccga tatcaaagat aaataaaatc gctcaaaacg atttttcctg
      601 tattcactgc cgttgcgcaa aatttatcta tttgttcaaa aaatgattga gtcttgactg
      661 gctcatccaa tgtggaaaaa tgtgactttt atcacataat agtgctaagt ctgaattttc
      721 cgggttatct caaaatggaa tacggttcga caaagatgga agagagactc tctcgcagcc
      781 ctggcggcaa actggctttg tgggctttct atacatggtg tggctatttc gtctgggcaa
      841 tggcgcgtta catctgggtg atgagccgga tccccgatgc ccccgtgtcg ggatttgaaa
      901 gcgatttagg ctctaccgcg ggaaagtggt taggggcact ggtcggattt ttatttatgg
      961 ctctggtcgg ggcattgtta ggcagcattg cctggtacac ccgacctcgt cccgcacgta
     1021 gcagacgcta tgaataaaaa ctgctaaggt ggcgactctt tggcaacgga gcgcgtatgg
     1081 acttacaaca ctggcaggca cagttcgaaa actggttgaa aaaccatcat cagcaccagg
     1141 acgcggccca tgacgtgtgt cattttcgcc gcgtctgggc tacggcacag aagctggcgg
     1201 cagatgacga tgtcgatatg ctggtgattt taaccgcctg ttattttcac gatattgtga
     1261 gcctggcgaa aaatcatccg caaaggcagc gttcttcaat cctggcggca gaagagacgc
     1321 gtcgcctgct ccgtgaagag ttcgagcaat ttccggcgga gaaaatcgag gccgtttgtc
     1381 atgtcattgc cgctcacagt ttcagcgcgc aaatcgcccc cttaacaacg gaagctaaaa
     1441 tcgtccagga tgcggatcgg ctggaagccc tgggggcgat tggcctggcg cgcgtgtttg
     1501 ccgtttcagg ggcattgggc gtggcgctgt ttgatggtga agatccgttc gcacagcatc
     1561 gcccgcttga tgataaacgc tacgcgctgg atcatttcca gactaagttg ctgaagctac
     1621 cgcaaaccat gcaaaccatg caaaccgcaa gaggcaagca gctggcgcag cacaacgcgc
     1681 attttttagt cgagtttatg gcgaagctca gtgccgaact ggcgggggag aatgaaggtg
     1741 tcgatcacaa ggtgatagat gcgttttcac ccgcgggata agcgcgtggc cctaaatggc
     1801 tgtaattatg ttaacctgtc ggccatctca gatggccggt taaatctatg caggaaaata
     1861 tatcagtaac cgattcatac agcaccggga atgccgcaca ggcaatgctg gagaaactgc
     1921 tgcaaattta tgatgttaaa acgctggtgg cgcagcttaa tggtgtgggt gagaatcact
     1981 ggagcgcggc aattttaaaa cgtgcgctgg cgaatgactc ggcatggcac cgtttaagtg
     2041 agaaagagtt cgcccatctg caaacgttgt tacccaaacc accggcacat catccgcatt
     2101 atgcgtttcg ctttatcgat ctatttgccg gaattggcgg catccgtcgc ggttttgaat
     2161 cgattggcgg acaatgcgtg tttaccagcg aatggaacaa acatgcggta cgcacttata
     2221 aagccaacca ttattgcgat ccggcgacgc atcattttaa tgaagatatc cgcgatatca
     2281 ccctcagcca taaagaaggc gtgagtgatg aggcggcggc ggaacatatt cgtcaacaca
     2341 ttcctgaaca cgatgtttta ctggccggtt tcccttgtca gccattttcg ctggctggcg
     2401 tatcgaaaaa gaactcgctc gggcgggcgc acggttttgc ctgcgatacc cagggcacgc
     2461 tgttttttga tgtggtacgc attatcgacg cgcgtcgtcc ggcgatgttt gtgctcgaaa
     2521 acgtcaaaaa cctgaaaagt cacgaccagg gtaaaacgtt ccgcatcatc atgcagacgc
     2581 tggacgaact gggctatgac gtggctgatg cagaagataa cgggccggac gatccgaaaa
     2641 tcatcgatgg taaacatttt ctgccgcagc accgtgaacg catcgtgctg gtgggttttc
     2701 gtcgcgatct taatctgaaa gccgatttta ctctgcgtga tatcagcgaa tgtttccctg
     2761 cacagcgagt gacgctggcg cagctgttgg acccgatggt cgaggcgaaa tatatcctga
     2821 cgccggtgct gtggaagtac ctctatcgat atgcgaaaaa acatcaggcg cgcggtaacg
     2881 gcttcggtta tggaatggtt tatccgaaca atccgcaaag cgtcacgcgt acgctgtctg
     2941 cgcgttatta caaagatggc gcggaaattt taatcgatcg cggctgggat atggccaaag
     3001 gtgagaaaga ctttgacgat ccgctgaatc agcaacatcg tccacgtcgg ttaacgcctc
     3061 gggaatgcgc gcgcttaatg ggttttgaag cgccgggaga agcgaaattc cgcattccgg
     3121 tttcggacac tcaggcctat cgccagttcg gtaactcggt ggtcgtgccg gtctttgccg
     3181 cggtggcaaa actgcttgag ccaaaaatca aacaggcggt ggcgttgcgt cagcaagagg
     3241 cacaacatgg ccgacgttca cgataaggcc actcgcagca aaaatatgcg cgcgattgcc
     3301 acgcgtgata cggcgattga gaagcgcctc gccagtctgt taaccgggca gggcctggcg
     3361 tttcgcgttc aggacgccag tctgcccgga agcccggatt ttgtcgttga tgaatatcgc
     3421 tgcgtgatat ttacccatgg ctgcttctgg catcatcatc actgctatct gtttaaagtg
     3481 cctgcgactc gaaccgagtt ctggctggag aagataggta aaaatgttga acgcgatcgc
     3541 cgcgatatca gtcgtttgca ggaactcggc tggcgcgtac tgattgtctg ggagtgtgca
     3601 ttacgtgggc gcgagaagct gacggatgaa gcgcttaccg agcgtctgga agagtggatc
     3661 tgcggcgaag gtgccagcgc gcagatcgac acgcagggga ttcatttact cgcttgatgc
     3721 gtcctgaata actggcgcaa ctacgggttt tgccgggaag agatattttc ccaacgtgac
     3781 cagtaccacc gcgaagacaa ttacgccgag cgccagccat tcaatcttcg acagcgtctc
     3841 tccgcccagt cccgtaccca gcaagaccgc gaccaccggg ttaacgtagg cgtagctggt
     3901 ggcgagagcc ggactgacat tacggattaa atacatataa gcgttgatgg cgataatcga
     3961 accaaacagc gccagatagc cgaccgcaag gaagcctgaa agggaaggga gcgccgtcag
     4021 tttttcacct gcaatcatcg acgcgatcat taacaccacg cctgccgcca gcatctcaat
     4081 cgcacccgcc atcatcccta caggtaaggt aatgcgcgag ccataaaccg agccaaacgc
     4141 ccagctaatc gagccgatta aaatcagaat cgcgccccac ggattgccgc ttaaatttcc
     4201 accgctattg agcatgatga ttccggcaag cccaatggcg atacccaccc attccaattt
     4261 gcgcgtttta atgccaaaca ggcggctgaa gcacagggta aagaggggca cggttgcaac
     4321 cacaacggcg gcgatgccgg aaggaacatt ttgatgctcg gcaaccgtca ccatgccatt
     4381 accgacagcc agcaataaca ggccaatcag cgcggcattg agcagcggac gtagcggggg
     4441 gagcttgtgt ccgcgcagta gcaaaaatgc catcaataaa ataccggctg ccaggaatcg
     4501 aacgcctgcc atcattaacg gaggccagct ttccacgcca atccgaatga caaaataggt
     4561 tgagccccaa atgatataca acgcaaacag cgcgccaaaa agcggtaaca actggcggaa
     4621 acgcataatc cctcacggtg gaaataaaaa ggtggttcat agtaaacgtg aaaatcattc
     4681 tgctggcgag agatataatt gcacttgatt gttaaaaaaa tgttgacctg tgaagcaagt
     4741 atcagagctg ccgtttttgc ttcatactta cacccttcaa caataaaaat gagagggaat
     4801 gcttttggcc gggagtagtt tactgacgtt gctcgatgat atcgccacac tactggacga
     4861 tatctccgtg atgggcaaac tggcggcgaa gaaaaccgcc ggtgtattag gggatgactt
     4921 atcgctcaat gcgcaacaag tttcaggcgt gcgggccaac cgggaacttc ccgtggtctg
     4981 gggcgtggcg aaaggatcgc tgattaataa agtgattctg gtgccgctgg cgctgatcat
     5041 cagtgcgttt atcccgtggg cgattacgcc tctgttgatg attggtggcg cgtttctctg
     5101 ctttgaagga gtagagaaag tgctgcatat gctggaggcg cgtaaacata aagaagatcc
     5161 ggcgcagagc cagcagcgtc tggagaagct ggcggcgcag gatccgctga agtttgaaaa
     5221 ggacaaaata aaaggggcga ttcgtaccga ttttatattg tctgccgaaa tcgtcgccat
     5281 cacgctgggg attgtggccg aagcgccgtt gcttaatcag gtgctggtgc tttcaggcat
     5341 cgcgctggta gtgaccgtgg gcgtctacgg tctggtaggg gttatcgtta agattgatga
     5401 cctggggtat tggctggcgg aaaaatccag cgcgctgatg caggcattag gtaaagggtt
     5461 attgatcatt gcgccctggc tgatgaaagc gttatcgatt gtcggcacgc tggcgatgtt
     5521 cctcgtcggc ggcgggattg tggtacatgg tattgcgccg ctgcatcacg ccattgaaca
     5581 tttcgccggg cagcaaagtg cagtggtggc gatgatatta ccgactgttt taaatctgat
     5641 tcttggattt atcatcggcg gcatcgtggt gctgggagtg aaagccgtag cgaaaatacg
     5701 cggtcaggca cattaatact cgcacaggct attatgcaaa attcgccatc ttcaactaag
     5761 gtgaacaagt ttcactcgaa aaaaggaggc gagtatgagc tttatggtta gtgaggaagt
     5821 tacggttaaa gagggcggcc cgcggatgat tgtcaccgga tactccagcg gtatggttga
     5881 gtgtcgttgg tatgacggtt acggggtcaa gcgggaagct tttcatgaaa ccgagcttgt
     5941 tccgggggag gggagtcgtt ctgcggaaga agtttgaaag ggtatcatta acaccaggcc
     6001 ggatcacttc cggcctggtg tgccaatgcc tgcaatgaat gatagtcgcg tcttatcatg
     6061 ccaccattat aaaattatcg ctgttttgcg gtacgctatc gccaggttat gccagaatca
     6121 taaaaaagca ggttgggagt cgtcagggtg cagcacgaga caaaaatgga aaaccagagc
     6181 tggttgaaaa aaatcgcacg ccgcctgggg cctggtcatg tcgttaatct ctgctttatc
     6241 gtggtattgc ttttttccac cttgctgacc tggcgtgaag tggtagtgct ggaagatgcc
     6301 tatatctcca gtcagcgtaa tcatctggaa aacgtagcca acgcgctcga taagcatttg
     6361 cagtataacg tcgacaaact gatctttttg cgtaatggca tgcgcgaagc tctcgtagcg
     6421 ccactggatt tcacttcact gcgtaatgct gtaaccgagt tcgaacagca tcgcgacgag
     6481 cacgcctggc aaattgaact caaccgacga cgcaccctgt cagtcaatgg cgtatcggat
     6541 gcattagtca gcgaggggaa tctcctgtct cgcgaaaatg aaagcctcga caatgaaatt
     6601 accgctgcac tggaagttgg ttacttgctg cgactggcgc acaacacctc gtcgatggtt
     6661 gaacaggcga tgtatgtctc gcgtgccgga ttttacgttt cgacgcagcc gaccttgttt
     6721 acgcgcaatg taccaacgcg ttattacggc tatgtcaccc aaccctggtt tatcggccat
     6781 tcgcaatgag aaaatcgtca ccgcgcggta cgctggttta cttcgcaacc ggaacacgcc
     6841 agcaatactg aaccgcaggt taccgtcagt gttccggtag acagtaataa ctactggtat
     6901 ggcgtgctgg ggatgagtat tcccgtgcgt accatgcagc aatttttaag aaacgccatc
     6961 gataaaaacc tcgatggtga gtatcagctc tatgacagta agctgagatt tttgacctct
     7021 tccaatcctg accatccaac agggaatatt tttgatcctc gtgaactggc cttgctggcg
     7081 caggcgatgg aacatgacac gcggggcggc attcgtatgg acagtcgcta tgttagctgg
     7141 gaacgtctgg accatttcga cggtgtgctg gtgcgcgtcc atacgctaag cgaaggcgtg
     7201 cgcggcgatt tcggcagtat cagcattgca ttaaccctgc tgtgggcgct ctttaccacc
     7261 atgttactca tctcctggta tgtgattcgc cggatggtta gcaacatgta tgttctgcaa
     7321 agctcgttgc agtggcaggc gtggcacgac accttaaccc gtttatataa tcgtggcgca
     7381 ctgtttgaaa aagctcgtcc gctcgcgaaa ttgtgtcaga cgcaccaaca tcctttttct
     7441 gtcattcagg tcgaccttga ccattttaaa gcgattaatg accgctttag tcatcaggcg
     7501 ggcgaccgtg ttctttctca tgctgccgga ttaattagca gttccttgcg tgcgcaggac
     7561 gttgccgggc gggtcggtgg tgaggagttt tgtgtgattc tgccaggcgc gagtctgacg
     7621 gaggctgcgg aagtcgcaga acgtattcgc ctgaagttaa atgaaaaaga gatgttgatt
     7681 gctaagagta cgacgatacg catcagtgcc tcgttggggg taagtagcag cgaggaagcc
     7741 ggtgattatg attttgaaca actccagtca ctggctgacc gtcggcttta tctcgctaaa
     7801 caggccgggc gtaatcgggt atgcgcgagc gataacgctt aacgagcgga gaaaaaatgg
     7861 tccagccctt cacgccatcc ttccggtcct tcacgctgcg ttcgccagac gcgggccgga
     7921 tcctcatcat gcagatgcac cccttcacgg tttagccctt tcacaatcac cgcgtaatcc
     7981 attacctcca gtaagggcgc atcgtttggc ccatcgccca ggccaagtgt ggttgggcgt
     8041 ttgcctgata attgttgata ggtcgcgata atccagttgg cggcctgatc ttttccggca
     8101 gaggcatcca ggacgtgcca gaagcgcgca ccctgcataa actgtaagcc cagttcgttc
     8161 agacgagcgg taaattgtgc catacgctcg tcactgtcgc gccagattag cgttaccgac
     8221 gcctcatgca gctgcgtcag cgccgcctgg ctacggctta atcccgtcca ttcggcgatg
     8281 gttgcatcgt cgacatcatc gaaagtcgtg aatttaaaat gttctttctc gcgtagcgta
     8341 tttaaaacct ggctgatttc gccatggcta atacctgaga tgatgcgtgg aaaaccgtct
     8401 atatcctgcc attgttcagc aagctggatc actgcgccat tttcggcaat cagtggtacc
     8461 ccttgtaacc ccaacgtttt ttgcaagtac agcatttccg ctgaagtttt actgctacag
     8521 agaatgacgg gaacattcgc ttcgcgtaaa cggctgagct agggggcagc cggttgccag
     8581 tcataactat gactgtccag cagggtgcca tcaagatcgc taaaaaccag tagtggttgt
     8641 tgaattgaaa acatggtcgc gcagttctcc ccttaccagg atgattctca aaacagcaca
     8701 aataatcaaa gatgattttt tcgggagcga tcccgttgac gccagagcca ccaaggggtg
     8761 acgcacgtca catttctatt cataagtagc gttaatcatt catatcgcga atattttctt
     8821 gtcagcgaaa aaaattgcgg ataaggtgat gaacacatca gatttcctgg tgtaacgaat
     8881 tttttaagtg cttcttgctt aagcaagttt catcccgacc ccctcagggt cgggattttt
     8941 ttattgtgca ttcaacgatt cacttcagag acgctgaagt catgaatatc cagttcgaag
     9001 gcatccgcca attcacgcca tgtatgatat tcacgtccat cgacgctaac gtgttcagaa
     9061 tcgttctggc tgcgatgaac ttatttcatt gatcgccgcc agcagggcat cgacatcgac
     9121 gctgacctca cgttttgcgg tatcgctgta ctcttttgcg gttttcatca tctttcctcg
     9181 caaccgttac tctgttgttg ccgccagtcg ttaagtccgc ctggcatctc ttaagtatag
     9241 accgtcgaga aaatcagcac tcgcgcgcgc ctggcgctgc cagaaaaacg aaattgttct
     9301 acactggcac aaagccacag gaggaaaacg atgaaggtga atgatcgggt aacagtcaaa
     9361 acggatggcg gtccgcgtcg tcctggcgtg gtactagcag ttgaggagtt tagtgaaggc
     9421 acaatgtacc tggtttcgct ggaagactac ccgctcggca tctggttctt taatgaagca
     9481 gggcatcagg acggtatctt tgtggagaaa gcagagtaat cttattcagc ctgactggtg
     9541 ggaaaccacc agtcagaatg tgttagcgca tgttgacaaa aataccatta gtcacattat
     9601 ccgtcagtcg gacgacatgg tagataacct gtttattatg cgttttgatc ttacgtttaa
     9661 tattaccttt atgcgatgaa acggtcttgg ctttgatatt catttggtca gagatttgaa
     9721 tggttccctg acctgccatc cacattcgca acatactcga ttcggttcgg ctcaatgata
     9781 acgtcggcat atttaaaaaa gaggttatcg ttgtctcttt tttcagaata tcgccaagga
     9841 tatcgtcgag agattccggt ttaatcgatt tagaacttat caataaattt tttctgacca
     9901 atagatattc atcaaaatga acattggcaa ttgccataaa aacgataaat aacgtattgg
     9961 gatgttgatt aatgatgtgc ttgatgtgct gactgttaga agcatcatgg atgaaacagt
    10021 cctcattaat aaacaccact gaagggcgct gtgaatcaca agctatggca aggtcatcaa
    10081 cggtttcaat gtcgttgatt tctctttttt taacccctct actcaacaga tacccggtta
    10141 aacctagtcg ggtgtaacta cataaatcca taataatcgt tgacatggca taccctcact
    10201 caatgcgtaa cgataattcc ccttacctga atatttcatc atgactaaac ggaacaacat
    10261 gggtcaccta atgcgccact ctcgcgat
//
LOCUS       DAJTNN010000144        10226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTNN010000144 DAJTNN010000000
VERSION     DAJTNN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.144"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            176..1789
                     /gene="trg"
                     /locus_tag="OO150_003200"
                     /pseudo
     CDS             176..1789
                     /gene="trg"
                     /locus_tag="OO150_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(1827..2749)
                     /locus_tag="OO150_003201"
                     /pseudo
     CDS             complement(1827..2749)
                     /locus_tag="OO150_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2966..4309
                     /locus_tag="OO150_003202"
     CDS             2966..4309
                     /locus_tag="OO150_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5517249.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGPLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEYRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4534..6189
                     /gene="opgD"
                     /locus_tag="OO150_003203"
                     /gene_synonym="mdoD"
     CDS             4534..6189
                     /gene="opgD"
                     /locus_tag="OO150_003203"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5517250.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6329..6553
                     /locus_tag="OO150_003204"
     CDS             6329..6553
                     /locus_tag="OO150_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5517251.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            6616..7152
                     /gene="rimL"
                     /locus_tag="OO150_003205"
     CDS             6616..7152
                     /gene="rimL"
                     /locus_tag="OO150_003205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5517252.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7147..8127)
                     /locus_tag="OO150_003206"
                     /pseudo
     CDS             complement(7147..8127)
                     /locus_tag="OO150_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8251..9243
                     /gene="tehA"
                     /locus_tag="OO150_003207"
     CDS             8251..9243
                     /gene="tehA"
                     /locus_tag="OO150_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5517253.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9240..9833
                     /gene="tehB"
                     /locus_tag="OO150_003208"
     CDS             9240..9833
                     /gene="tehB"
                     /locus_tag="OO150_003208"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5517254.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10136..>10226
                     /locus_tag="OO150_003209"
     CDS             10136..>10226
                     /locus_tag="OO150_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5517255.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDK"
BASE COUNT         2621 a         2387 c         2587 g         2631 t
ORIGIN      
        1 gtcattcgga ttagccagtt tagccattcg ttactctctt cattccaata gcattaattt
       61 tctctgcaat aattgttgta aaaatgtgac gcaaagaggt ttttggtcat aagtaattac
      121 cgtcaagtgc cgatgacttt ctatcaggag taaacctgga cgagagacaa cggtaatgaa
      181 tacaactccc tcacagcgat taggtttttt gcatcacatc aggctggttc cgttatttgc
      241 ctgcattctt ggcggtatct tagttctatt cgcattaagt tctgctctgg caggccgatc
      301 gcgatcagcg tgatgttact gcggagattg agattcggac cgggttagcg aacagttcag
      361 attttttgcg ttcagcccgg atcaatatga ttcaggccgg ggctgcgagt cgtattgcgg
      421 aaatggaagc aatgaagcga aatattgcgc aagccgaatc ggagattaaa cagtcgcagc
      481 aaggttatcg tgcttatcag aatcgaccgg tgaaaacacc tgctgatgaa gccctcgaca
      541 ctgaattaaa tcaacgcttt caggcttata tcacgggtat gcaacctatg ttgaaatatg
      601 ccaaaaatgg catgtttgaa gcgattatca atcatgaaag tgagcagatc cgaccgctgg
      661 ataatgctta taccgatatt ttgaacaaag ccgttaagat acgtagcacc agagccaacc
      721 aactggcgga actggcccat cagcgcaccc gcctgggtgg gatgttcatg attggcgcgt
      781 ttgtgcgtgc cctggtcatg acgctgataa catttatggt gctacgtcgg atcgtcattc
      841 gtccactgca acatgccgca caacggattg aaaaaatcgc cagtggcgat ctgacgatga
      901 atgatgaatc ggcgggtcgt aatgaaatcg gtcgcttaag tcgtcattta cagcaaatgc
      961 agcattcact ggggatgaca gtagggactg ttcgacaggg cgcggaagag atttatcgtg
     1021 gcaccagcga aatttcagct ggcaatgcgg acctgtcatc tcgcaccgaa gaacaagcgg
     1081 cggctatcga acaaactgcc gccagcatgg agcaactcac tgcgacggtg aaacagaatg
     1141 cggataacgc gcatcatgcc agcaaactgg cgcaagaggc ttctattaaa gccagcgatg
     1201 gcgggcagac ggagtaaaaa cgatgggcgc tatctccacg agttcgaaga aaatttctga
     1261 gatcaccgcc gtcatcaaca gtattgcttt ccagacgaat attctggcac tgaatgctgc
     1321 cgttgaagcc gcgcgagcgg gtgagcaagg gcgtggattt gccgttgtcg ccagcgaagt
     1381 acggacactc gcaagtcgca gcgctcaggc ggcgaaagag attgaaggct tgatcagtga
     1441 atcagtcagg ttaattgacc tggggtcgga tgaggtggca acggccggga aaaccatgag
     1501 cactattgtt gatgccgtcg cgagtgtcac acatatcatg caggaaatcg ccgccgcctc
     1561 ggatgaacaa agtagaggca taacgcaggt tagccaggcg atttctgaaa tggataaggt
     1621 gacgcaacag aatgcttctc tggtagaaga ggcctcagcg gcggcggtgt cccttgaaga
     1681 acaggcggca cgattaactg aggcggtgga tgtattccgt ctgaacaaac attctgtgtc
     1741 ggcagaacct cgcggagcgg gtgaaccagt tagtttcgct acggtgtgaa aatgttcaag
     1801 gagggatcga cagatccctt cacctttcag aacggcattg attttcgaat agcgttaatc
     1861 atcaactggc aaccagaaga gaacatcgca tctacgcggg tcagtattcc aatcggttcg
     1921 cctgcaccat gtcccggaac aggctgggcc accagcgtgg catgacgcag gtcgtctttt
     1981 accgcgccag aagggacaaa ccacacgtag tcgtattcaa ccgtaagttg acgagatagc
     2041 gaagcagaca gcgtttcgat acaacccgaa ggaattttac atccctggct ctgtactaat
     2101 gcatctgaat gctggcgtgg cgcagtgcct tctggtgata caacgaccgg ccatttcagc
     2161 actcggctta gcgttacgtt ctcctgaagt agcgggtgat tagggcggac aaccagcttc
     2221 agcgattcaa gaaacagcag ttcgtaatta agcccggtca tcagttcagg atctgacatc
     2281 cgaccaatgc cgatatcgat ttccccggtt ttcaaatccg ccagaatcat agggttactc
     2341 attgtcgcaa cttgcaaggt cgtctctttt tgttgctgat gaaactgacc tataaccgaa
     2401 ggtaatatcc ccagtgccgc agtaggtagt gcaccaatcc tgacgacatc attattaaga
     2461 ccttctttac gatgaagcga ctgtccggca gtgttgatgg cgtcaagaac tctgactgca
     2521 tgcgttaaaa attgttcgcc gggtaaggta agttgcgccc cctgacgacc acgctcaaac
     2581 aagcgagcgc ccgtcagctg ctacagttca ttcaatgtct tagagagcgc aggttgactc
     2641 aaattaaggg tttcagccgc gcccccaaag ttccttgttg tgcgacagct acgaatgtat
     2701 gaaggtggcg caaacggatg cgctgactaa acagactatt tttttccata agcgatgtta
     2761 aaaacgaagc ggtgtcgctg acaagtgaag ttgtttgatt atgataactt gattgcaaaa
     2821 tataattaac aattaaagca attgtgttac agcaaaatgg ataatattga tgttttcgcg
     2881 gcgagatcac agtttgtaaa ttcttcccgc cagagtgaat gcggttacct acactccaga
     2941 ttactgacca ctggaggcag acactatggc gaacagcatc acggcggatg agattcggga
     3001 acagttttcg caggcaatgt cagccatgta ccagcaagaa gttccgcagt acggcccgct
     3061 gctggaactg gtagctgatg tgaatctggc tgtgctggaa aacaatcctc aactgcacga
     3121 aaaaatggta aatgcagatg agctggcgcg actgaatgtt gaacgtcatg gggcgattcg
     3181 cgttgggact gcacaagagc ttgctactct tcggcggatg tttgccatta tggggatgta
     3241 cccggtgagc tattacgatc tctcgcaggc aggggggccg gtacattcga cagcatttcg
     3301 gcccattgat gatgcttctc tggcgcgtaa tcccttccgc gtttttacct ccttactccg
     3361 ccttgagctt atcgagaacg aaattttgcg ccagaaagcg gcggagattc tgcgtcagcg
     3421 cgatatcttc accccacgtt gtcgacaact gttagaggaa tatgagcagc agggcggttt
     3481 taacgaaaca caggcacagg aatttgtgca agaggccctg gaaacgtttc gctggcacca
     3541 gtcagcaacg gtagatgaag aaacctatcg cgccttgcac aacgaatatc ggttgattgc
     3601 tgatgtggtc tgttttcctg gatgccatat caaccacctg acgccacgta cgctggatat
     3661 tgaccgggtg cagtcgatga tgcctgaatg cggaattgaa cccaaaattc tcatcgaggg
     3721 gccgccgcgc cgcgaggtac cgattttact acgccagacc agctttaaag cactggaaga
     3781 gacggtgttg tttgcggggc agaaacaggg cacgcatacc gcgcgctttg gtgaaattga
     3841 gcagcgtggc gtggcattaa cgccgaaagg gcgacaactg tatgatgatc ttctgcgtaa
     3901 cgctggaacc gggcaggata atctcactca ccaaatgcat ttacaggaaa ccttccgcac
     3961 ttttcctgac agtgagtttt taatgcgtca gcaagggttg gcatggttcc ggtaccgtct
     4021 gacgccttcg ggtgaggcgc atcgtcaggc gattcatctt ggagacgatc cacagccttt
     4081 aattgaacgt ggttgggtag tggcgcaacc catcacctat gaagatttct tgcccgttag
     4141 cgcggcgggg atcttccagt caaatctggg taatgaaacg cagacacgca gtcacggtaa
     4201 tgccagtcgc gaagcatttg agcaggcgtt gggttgtccg gttttggatg agttccagct
     4261 ttaccaggaa gcggaagaac gcagtaaacg tcgctgtggt ttgctttaaa ttctgaccat
     4321 ccgcctttgc aaaaatttgc ctgattttac aaacgaatca ggctcatccc atcgacataa
     4381 aaaaaatgcc gatttatgca tattctctca gttcaacaat tggattatta ataaatattg
     4441 tctagagtga gcggtcataa ataagcactt tcttgccgct gaaaacgacc agcgcgggac
     4501 cattcacaac accagaagga ctcactttca ggtatggatc gtagacgatt tattaaaggt
     4561 tcaatggcta tggccgccgt gtgcggtacc agcggcattg cttctctttt ttctcaggcg
     4621 gcattcgcgg cagattctga tattgccgac gggcaaaccc agcgttttga cttctccatt
     4681 ctacagtcaa tggcgcacga cttagcgcaa acagcgtggc gtggtgcgcc tcgtccgtta
     4741 cctgacacgc tggcgacaat gacgccgcag gcttataaca gtattcaata cgacgccgaa
     4801 aaatcgctct ggcataacgt tgagaaccgt caactggacg ctcagttctt ccatatggga
     4861 atgggattcc gtcgccgcgt tcgtatgttt tctgtagatc cagcaacaca tctggcgcgt
     4921 gaaattcact ttcgcccgga gttgttcaaa tacaacgatg cgggtgttga tacaaaacaa
     4981 ttagaagggc aaagcgatct cggctttgcc ggttttcgcg tgtttaaagc ccccgaactg
     5041 gcgcgccgtg atgtagtatc atttctcggc gcgagttatt tccgcgccgt tgatgataca
     5101 tatcaatacg gtttgtcggc ccgcggcctg gcgatcgaca cttacaccga cagtaaagaa
     5161 gagttccccg actttaccgc cttctggttt gatacggtaa aaccgggggc aactaccttt
     5221 accgtttatg cgttgctcga tagcgccagc attactggtg cctataagtt cactatccat
     5281 tgtgagaaaa gtcaggtgat tatggatgtg gaaaatcacc tgtatgcgcg caaagacatt
     5341 aaacagctgg gcattgcgcc gatgaccagt atgttcagct gcggtactaa tgaacgtcgg
     5401 atgtgcgata caattcatcc gcaaattcat gactctgatc gtctgtccat gtggcggggc
     5461 aacggcgagt ggatttgccg tccgctgaat aatccgcaaa aattgcagtt caatgcttac
     5521 accgacaaca acccgaaagg gtttggttta ttgcaactgg atcgtgactt ctcccattat
     5581 caggacatta tgggctggta taacaaacgc ccaagtctgt gggtggaacc gcgtaacaag
     5641 tggggtaagg gcaccatcgg cctgatggaa atcccaacaa cgggcgaaac gctggataac
     5701 attgtctgtt tctggcagcc agaaaaagct gtaaaagcag gtgatgagtt tgcattccag
     5761 tatcgtctgt actggagtgc gcaaccgcct gttcattgcc cattagcgcg cgttatggcg
     5821 acgcgtaccg gcatgggcgg tttcccggaa ggttgggcgc caggtgaaca ctatcccgaa
     5881 aaatgggcgc gtcgttttgc cgtcgatttc gttggtggtg atctgaaagc tgccgcgcca
     5941 aaaggcattg agccggtgat tacgctttcc agtggggaag cgaagcaaat cgaaattctc
     6001 tatattgaac ccatcgatgg ttatcgtatt cagtttgact ggtatccgac ttcggactcc
     6061 actgatccgg tcgatatgcg gatgtatcta cgttgtcagg gggacgctat cagtgaaaca
     6121 tggctgtatc agtatttccc gccagcgccg gataaacgtc agtatgttga cgaccgcgtg
     6181 atgagttaat tgttttttct tcggcacctt cttcgggagg tgccgtctgg ttaaacacga
     6241 tcccgctcgc atttttccct aagttaaatg agtaatctga tggtgtgtat ttcagataca
     6301 ccttgtcagc cactaacagg gagtgcgtat gtttccagaa taccgagatt taatatcccg
     6361 tctgaaaaac gaaaatcctc gctttatgtc cttgttcgat aaacacaata aacttgatca
     6421 tgaaattgcc agaaaggaag gtttcgacgg tcgagggtac aatgcggaag tggtccgcat
     6481 gaaaaaacaa aagctacagt taaaagatga gatgctcaaa atcctgcagc aggagagcgt
     6541 caaaggggtg taaactttcc taagccgcca gccaggcggc ttttttaaca acagcatgga
     6601 ttgactggag ataagatgac tgaaacgata aaagtaagcg aatcacttga attacatgct
     6661 gttgcggaaa gtcacgtcac acctctttat cagttaatct gtaaaaataa aacctggtta
     6721 cagcagtcgc taaactggcc gcagttcgtt cagagtgaag aggacacgcg aaaaacggtg
     6781 cagggtaatg tgatgttgca tcaacgcggc tatgccaaaa tgttcatgat tttcaaagaa
     6841 gatgaactta tcggcgttat ctcgtttaat cgtattgaac cactgaataa aactgctgaa
     6901 atcggctact ggctggacga atctcatcag gggcagggga tcatttctca ggcgctgcag
     6961 gcattgattc atcattacgc tcagtctggt gaacttagac gcttcgtgat caaatgtcgg
     7021 gtggacaatc cgcaaagcaa ccaggtcgct ttgcgcaatg gttttatcct tgaaggttgc
     7081 ctgaaacagg ctgagttcct gaatgatgcc tatgatgatg tgaacttata cgcgcgtatt
     7141 atcgattcat aataaccctg ccagcggcgt tcgcgtaatg cgctcctcgc cgttaatgac
     7201 ttttggacca cggacatgaa ccgtgtcgcc atcaaaggct tcaacgacgg cgtggtcggt
     7261 taactccaca tggttttcaa ttatcaccgc acctgaaata cggctctcac cttgtattac
     7321 aacgtgctcg tcgagcagaa tcggttcacc acgtaccacg gcatttccgc caatcaagac
     7381 atgatgtttt aacacacaat taccttccac aatggcatat tccgccacct gcgaactgta
     7441 atgaatcgtc ggaatggcat cttcttctat gccagctttc acctgcgcgt gaccgtagac
     7501 tttagcgcaa tcgcatagcc agacgttgtt ctcttcatta ccttcaacac tggcaaaatc
     7561 aaaaacttcg gcgcgatgtt caataaaagc ataccggacg acggcatcgc cataaatttg
     7621 tgcctggtgg acaatacgcg aggcgctaac ccttgcgcga tcataaattt ggagcaggag
     7681 ttgatggtcg ggcgttaagc cttgtgcggc gacgatcata gaatgttgat caattaaggc
     7741 gtgaccaaat attctacatt gcccgtaaac taatgagtca tgaatggtga cactgtcact
     7801 tatatatgca ccctgactga tttcactatt atcgatccag acgttatccg ttgcgtaaac
     7861 ctctccccac agcacgctgg tgccggtgat acgcgtatta ccagaaatca ccgttccgcc
     7921 gaacgcaata gcattctggt cgtaaatcca gcaatttcct tcctatgcta acaccgtttc
     7981 gcgatcgatc cagccgccag cggttcctgc tatcacatcg ttaaaatcgc taatggcgat
     8041 aatctgccgt aataacacac tttttttagt gccatcttcc tgataactaa aggctcgctg
     8101 ttcttcactt agacgatatt tgcgcatagc gttttctcac aggtgactta ctataaccgt
     8161 agcaaattct gcggctctgg ctatgctcga gaaaattcca taaaatgcat ttcaaatata
     8221 ctttataaat taaacaaaat gagtaagaag atgcagagcg ataaagtgct caatttgccg
     8281 gcaggctact ttggtattgt gttggggacg atagggatgg gatttgcctg gcgctatgcc
     8341 agccaggttt ggcaggtcag ccactggtta ggggatgggc tggtgattct ggcgatgatc
     8401 atctggggat tattaactag cgcatttatt acccgactca tacgctttcc gcatagcgtg
     8461 ctggcggaag ttcgccatcc agtgctgagc agttttgtga gtttgtttcc ggcaacgacg
     8521 atgctggtgg cgattggttt tgttccgtgg tttcgcccac tggcggtgtg cctgttcagt
     8581 tttggtgtcg tggttcagtt ggcttatgcc gcctggcaaa ctgcgggatt atggcgcgga
     8641 tctcaccctg aagaagctac catgcctgga ctgtatctgc cgacagttgc caacaacttt
     8701 atcagcgcaa tggcctgtgg tgcgttgggc tacaccgacg ccggtctggt gtttttaggc
     8761 gcaggcgttt tctcatggct aagccttgaa ccggtgatct tgcagcgtct gcgcagttcg
     8821 ggagaattac ccacggcact gcggacatca ctcggcattc agctcgctcc tgcgctggtg
     8881 gcctgtagtg cctggttgag cgtcaacggc ggcgagggtg atacgctggc gaaaatgctt
     8941 tttggttatg gactgctgca actgctgttt atgctacgtc tgatgccatg gtatctctcc
     9001 cagccattta atgcttcatt ctggagtttc tcgttcggcg tatctgcact ggcaaccacc
     9061 ggtttgcatc tggggagtgg cagcgaaaat ggatttttcc atacgctggc ggtaccgctg
     9121 tttatcttta ccaattttat tattgcaata ctgctcatcc gtacttttgc gcttctgatg
     9181 cagggaaaat tgttagtcag aaccgagcgc gctgttttaa tgaaagcaga ggacaaagaa
     9241 tgatcattcg tgacgaaaac tattttactg ataaatatga attaacccgc acacactctg
     9301 aagtactgga agcggtgaaa gtggttaaac cgggtaaaac gctggatctg ggctgtggca
     9361 atggtcgtaa cagtctttac ctggcagcca atggttatga tgttgacgca tgggataaaa
     9421 atgccatgag tatcgccaat gtagagcgca ttaaatccat tgaaaatctg gataatttac
     9481 acacccgagt cgttgatctg aataacctca catttgatag acagtacgat tttattcttt
     9541 cgactgtggt actgatgttc cttgaggcta aaaccatccc agggctgatt gccaatatgc
     9601 aacgttgcac taaacttggt ggttacaacc tgattgtggc ggcgatggat accgctgatt
     9661 atccatgtac cgtcggcttc ccgtttgcct tcaaagaggg agaattacgt cgatattacg
     9721 aaggctggga gagggtgaaa tacaatgaag acgtcggcga gctgcaccgc accgacgcca
     9781 acggtaatcg tattaaactg cgtttcgcca cgatgctggc acgtaaaaaa tgacccgcta
     9841 agaataaggc gcgtgaaaat tccccacgct gagatgattt actgttcttc ttttcggtaa
     9901 gcatattttt tatcgaaggg atgtgaaatt aatcacagta gtcgaagttt tttagcagct
     9961 taacttactg aaatttaact actgatgatt gacttagccc ctttttcggc attgactatg
    10021 tcgtctgaaa aggggctgaa aaatttattt tcaccaacac tttttttgcc acaacacgaa
    10081 gcggcgcttt ttgctataac ttaaaaagta atataatcat ctcaggaaac tattcatgcg
    10141 taccacatca tttgcgaaag ttgcagcttt atgcggcttg ttggctctgt ctggttgtgc
    10201 ctctaaaatc acccagccag acaaat
//
LOCUS       DAJTNN010000145        10213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTNN010000145 DAJTNN010000000
VERSION     DAJTNN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.145"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            141..1997
                     /gene="kup"
                     /locus_tag="OO150_003210"
     CDS             141..1997
                     /gene="kup"
                     /locus_tag="OO150_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5517256.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSLTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2164..2583
                     /gene="rbsD"
                     /locus_tag="OO150_003211"
     CDS             2164..2583
                     /gene="rbsD"
                     /locus_tag="OO150_003211"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5517257.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2591..4060
                     /gene="rbsA"
                     /locus_tag="OO150_003212"
                     /pseudo
     CDS             2591..4060
                     /gene="rbsA"
                     /locus_tag="OO150_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            4065..5030
                     /gene="rbsC"
                     /locus_tag="OO150_003213"
     CDS             4065..5030
                     /gene="rbsC"
                     /locus_tag="OO150_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5517258.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5055..5941
                     /gene="rbsB"
                     /locus_tag="OO150_003214"
                     /pseudo
     CDS             5055..5941
                     /gene="rbsB"
                     /locus_tag="OO150_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6067..6996
                     /gene="rbsK"
                     /locus_tag="OO150_003215"
     CDS             6067..6996
                     /gene="rbsK"
                     /locus_tag="OO150_003215"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5517259.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7000..7992
                     /gene="rbsR"
                     /locus_tag="OO150_003216"
     CDS             7000..7992
                     /gene="rbsR"
                     /locus_tag="OO150_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5517260.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7958..9385)
                     /gene="mdtD"
                     /locus_tag="OO150_003217"
     CDS             complement(7958..9385)
                     /gene="mdtD"
                     /locus_tag="OO150_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5517261.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     RATWHWIFLINIPIGIAGLLYARKHMPNFTTAQRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9408..10100)
                     /locus_tag="OO150_003218"
     CDS             complement(9408..10100)
                     /locus_tag="OO150_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5517262.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KTCQALLRSPDK"
BASE COUNT         2486 a         2457 c         2702 g         2568 t
ORIGIN      
        1 aaggggggta tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaaacat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatcca atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgttgt cgattacggg ggtcgaggcg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgctgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccgctgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtgct gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggtt ggttgccatt gagcctcggt acggtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgtaccgaag
     1681 acgcgccata tgtccataat gtccgtcggg tacagattga acaactgtcg ctcactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgaaacg cccgtggtat ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta
     1921 atgcgctgcg tgcaccagat caatttgaaa tcccgccaaa cagggttatc gaactgggta
     1981 ctcaggtcga aatctaacgc cagacgcctc ctttctccat aagggggcgt ttttgttttc
     2041 atggttaatc accatgtaaa acgtttcgag gttgatcaca tttccgtaac gtcacgatgg
     2101 ttttcccaac tcagtcagga ttaaactgcg ggtcagcgaa acgtttcgct gatggagaaa
     2161 aaaatgaaaa aaggcaccgt tcttaattct gatatttcat cggtgatctc ccgtctggga
     2221 cataccgata cgctggtggt gtgtgatgct ggtttaccca tccccaaaag tacaacgcgt
     2281 atcgatatgg cattaaccca gggtgtacct tcttttatgc aggtgctggg cgtcgttaca
     2341 aatgaaatgc aggtcgaggc tgccattatc gcggaagaga tcaaacaaca taatccgcaa
     2401 ctccacgaaa cgttgctcac tcaccttgag cagctgcaaa aacaccaggg aaataccatt
     2461 gaaattcgtt acaccacgca tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta
     2521 attcgcagcg gagaatgttc tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc
     2581 tgaggccgtc atggaagcat tacttcagct taaaggcatc gataaagcct tcccgggcgt
     2641 aaaagccctc tcgggcgctg cgttaaatgt ctatccgggc cgcgtgatgg cgctggtggg
     2701 cgaaaacggc gcgggtaaat ccaccatgat gaaagtgctt actggtatct atgctcgcga
     2761 tgccggcacg cttttatggc tggggaaaga aacgacattt accgggccga aatcttccca
     2821 ggaagccggg attgggatta tccatcagga actgaacctg atcccgcagt tgaccattgc
     2881 cgaaaacatt ttcctcggtc gtgagtttgt taatcgcttt ggcaaaattg actggaaaac
     2941 catgtatgcc gaagcggata aattgctggc taaacttaac ctgcgcttta aaagcgacaa
     3001 gctggtgggc gatctttcca tcggtgacca gcaaatggtt gaaatcgcca aagtgctgag
     3061 ctttgagtcg aaagtcatca ttatggatga accgaccgat gcgctgaccg ataccgaaac
     3121 cgaatccctg ttccgcgtca tccgcgaact aaaatcgcaa ggccgcggta ttgtctatat
     3181 ctcccaccgc atgaaagaaa tcttcgagat ttgcgatgcc gttaccgttt ttcgtgacgg
     3241 gcaatttatt gctgagcgcg aagtggcatc actgaccgaa gattcgctga ttgagatgat
     3301 ggtgggtcgc aagctggaag atcaatatcc acacctggac aaagcgccgg gagatatccg
     3361 cctgaaagtc gataatctct gcggacctgg cgttaacgat gtctctttta ctttacgcaa
     3421 aggcgaaatt cttggcgtct ctggtttgat gggcgcaggt cgtaccgaac tgatgaaagt
     3481 gctctacggc gcactaccgc gcaccagcgg ttacgtcacc ctggatgggc atgaagtcgt
     3541 tacccgttca ccgcaggatg gcctggcaaa cggcattgtg tatatctccg aagaccgtaa
     3601 acgtgacggt ttagtgttgg gcatgtcagt aaaagagaac atgtcgctga cagcgctgcg
     3661 ctacttcagc cgcgctggcg gcagtttgaa gcatgccgat gaacagtagg ctgtgagtga
     3721 tttcattcgt ctgtttaatg tgaaaacacc gtcgatggaa caggcaattg gtctgctttc
     3781 cggtggcaat cagcaaaaag tgttgatcct tgatgaacct acccgtggcg tagatgtcgg
     3841 cgcgaaaaaa gagatctatc aactgattaa ccagttcaaa gccgatggct tgagcatcat
     3901 tctggtgtca tcggagatgc cagaagtatt aggcatgagc gatcgcatca tcgtcatgca
     3961 tgaagggcat ctcagcgggg aatttactcg tgagcaggcc acccaggaag tgttaatggc
     4021 tgccgctgtg ggcaagctta atcgcgtgaa tcaggagtaa aaaaatgaca acccagactg
     4081 tctctggtcg ccgttatttc acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc
     4141 tgctggtgct gatcgcgatt gtctcgacgt taagcccgaa ctttttcacc atcaataact
     4201 tattcaatat tctccagcaa acctcggtga acgccattat ggcggtcggg atgacgctgg
     4261 tgatcctgac ttcgggcatc gacttgtctg taggttctct gttggcgcta accggcgcag
     4321 ttgctgcatc tatcgtcggc attgaagtca atgcgctggt ggctgtcgct gctgctctcg
     4381 cgttaggtgc cgcaattggt gcggtaaccg gggtgattgt agcgaaaggt cgcgttcagg
     4441 cgtttatcgc tacgctggtt atgatgcttt tactgcgcgg cgtgaccatg gtttatacca
     4501 acggtagccc agtgaatacc ggctttactg agaacgccga tctgtttggc tggtttggta
     4561 ttggtcgtcc gctgggcgta ccgacgccag tctggatcat ggggattgtc ttcctcgcgg
     4621 cctggtacat gctgcatcac acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg
     4681 aagcggcaac gcgtctttct ggtatcaacg tcaataaaat caaaatcatc gtctattctc
     4741 tttgtggtct gctggcatcg ctggccggga ccattgaagt ggcgcgtctc tcctccgcac
     4801 aacccacggc ggggactggc tatgagctgg atgctattgc tgcggtggtt ctgggcggta
     4861 cgagtctggc gggcggaaaa ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg
     4921 gcttccttaa taatggattg aatttgttag gtgtttcctc ctattaccag atgatcgtca
     4981 aagcggtggt gattttgctg gcggtgctgg tagacaacaa aaagcagtaa taacgactac
     5041 aggacatctt gaatatgaac atgaaaaaac tggctaccct ggtttccgct gttgcgctaa
     5101 gcgccaccgt cagtgcgaat gcgatggcaa aagacaccat cgcgctggtg gtctccacgc
     5161 ttaacaaccc gttctttgta tcgctgaaag atggcgcgca gaaagaggcg gataagcttg
     5221 gctataacct ggtggtgctg gactcccaga acaacccggc gaaagagctg gcgaacgtgc
     5281 aggacttaac cgttcgcggc accaaaattc tgctgattaa cccgactgac tccgacgcag
     5341 taggtaatgc tgtgaagatg gctaaccagg cgaacatccc ggttatcact cttgaccgcc
     5401 aggcaacgaa aggtgaagtg gtgagccaca ttgcttctga taacgtactg ggtggcaaaa
     5461 tcgccggtga ttacatcgcg aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag
     5521 gcattgctgg tacatccgca gcccgtgaac gtggcgaagg cttccagcag gccgttgctg
     5581 ctcacaagtt taatgttctt gccagccagc agattttgat cgcactaaag gtttgaacgt
     5641 aatgcagaac ctgttgaccg ctcatccgga tgttcaggct gtattcgcgc agaatgatga
     5701 aatggcgctg ggcgcgctgc gcgcactgca aactgccggt aaatcggatg tgatggtcgt
     5761 cggatttgac ggtacaccgg atggcgaaaa agcggtgaat gatggcaaac tagcagcgac
     5821 tatcgctcag ctacccgatc agattggcgc gaaaggcgtc gaaaccgcag ataaagtgct
     5881 gaaaggcgag aaagttcagg ctaagtatcc ggttgatctg aaactggttg ttaagcagta
     5941 gttttaatca ggttgtatga cctgatggtg acataaatac gtcatcgaca gatgaacgtg
     6001 taatataaag aaaagcaggg cacgcgccac cctaacacgg tggcgcattt tatggacatc
     6061 ccgaatatgc aaaacgcagg cagcctcgtt gttcttggca gcattaatgc tgaccacatt
     6121 cttaatcttc aatcttttcc tactccaggc gaaaccgtaa ccggtaacca ctatcaggtt
     6181 gcatttggcg gcaaaggcgc gaatcaggct gtggctgctg ggcgtagcgg tgcgaatatc
     6241 gcgtttattg cctgtacggg tgatgacagc attggtgaga gcgttcgcca gcagctcgcc
     6301 actgataaca ttgatattac tccggtcagc gtgatcaaag gcgaatcaac aggtgtggcg
     6361 ctgatttttg ttaatggcga aggtgagaat gtcatcggta ttcatgccgg cgctaatgct
     6421 gccctttccc cggcgctggt ggaagcgcaa cgtgagcgta ttgccaacgc gtcagcatta
     6481 ttaatgcagc tggaatcacc actcgaaagt gtgatggcag cggcgaaaat cgcccatcaa
     6541 aataagacta tcgttgcgct taacccggct ccggctcgcg aacttcctga cgaactgctg
     6601 gcgctggtgg acattattac gccaaacgaa acggaagcag aaaagctcac cggtattcgt
     6661 gttgaaaatg atgaagatgc agcgaaggcg gcgcaggtac tgcatgaaaa aggtatccgt
     6721 actgtactga ttactttagg aagtcgtggt gtatgggcta gcgtgaatgg tgaaggtcag
     6781 cgcgttcctg gattccgggt gcaggctgtc gataccattg ctgccggaga tacctttaac
     6841 ggcgcgttaa tcacggcgtt gctggaagaa aaaccattgc cagaggcgat tcggtttgcc
     6901 catgctgcag ctgcgattgc cgtaacacgt aaaggcgcac aaccttccgt accgtggcgt
     6961 gaagagatcg acgcattttt agacaggcag aggtgacgct tggctacaat gaaagatgtt
     7021 gcccgcctgg cgggcgtttc tacctcaaca gtttctcacg ttatcaataa agatcgcttc
     7081 gtcagtgaag cgattaccgc caaagttgaa gcggcgatta aagaactcaa ttacgcgcca
     7141 tcagccctgg cgcgtagcct caaactcaat caaacacata ccattggcat gttgatcact
     7201 gccagtacca atcctttcta ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa
     7261 cgcggttata gtctcgtcct ttgcaatacc gaaggcgatg aacagcggat gaatcgcaat
     7321 ctggaaacgc tgatgcaaaa acgcgttgat ggcttgctgt tactgtgcac cgaaacgcat
     7381 caaccttcgc gtgaaatcat gcaacgttat ccgacagtgc ctactgtgat gatggactgg
     7441 gctccgttcg atggcgacag cgatcttatt caggataact cgttgctggg cggagactta
     7501 gcaacgcaat atctgatcga taaaggtcat acccgtatcg cctgtattac cggtccgctg
     7561 gataaaactc cggcgcgcct gcggttggaa ggttatcggg cggcgatgaa acgtgcgggt
     7621 ctcaacattc ctgatggcta tgaagtcact ggtgattttg aatttaacgg cgggtttgac
     7681 gccatgcgcc aactgttatc acatccgctg cgtcctcagg ccgtctttac cggaaatgac
     7741 gctatggctg ttggcgttta ccaggcgtta tatcaggcag agttacaggt tccgcaggat
     7801 atcgcggtga ttggctatga cgatatcgaa ctggcaagct ttatgacgcc accattaacc
     7861 actatccacc aaccgaaaga tgaactgggg gagctggcga ttgatgtact catccatcgg
     7921 ataacccagc cgacccttca gcaacaacga ttacaactta ctccgattct gatggaacgc
     7981 ggttcggctt agatttacgc tgtcttttga tcaaattatt accatcggtt gttttcagaa
     8041 gcatgaacat tgctgctgaa gcaacagtaa taatgcccat cgtgataaac gtatagtgga
     8101 attgttcgac agtcgttgtg ccttccatcc cttcataaac gcgaaggacg gccgcactta
     8161 cagcaacgcc taaactaatc gacagttgct gcgtgaccgc cagaacactg ttaccgctgc
     8221 tggcgttgtc atcggtcaga tcggcaagtg tgatggtatt catcgcggta aattgcgtcg
     8281 acatagccat ccctaatata aacaacggca agatcagcat ccatatcgcc attgccggtg
     8341 attgcaaaga gaactgagcg atcattagcc caataatcac cgtgatcccc actaacgtat
     8401 ggcgatagcc cagacgacgt aagacttggg taaccatcga ttttgcaata atggaaccta
     8461 acgctgtcgg tgccatcata cagccggcaa taaacgcctg ataaccaaat cctacctgta
     8521 acatcaatgg cataaggaac ggtacactac cggtccccag acgggttgca atattgccta
     8581 cgataccgat cgagaaagtg cgggttttaa ataaatctaa tgaaattaat gggtttggcg
     8641 tgtgtcgcgc atggagaata tagagaagca gtaacccgat gctggtgaca attaccgtca
     8701 aggcaatcca gctggcgaca atcttttccc cgaatagctc tattccgctt gagaagagaa
     8761 caaggctgag gccaaacagc aaaaagccag tgatatcgaa tctgcgttgt gcggtggtga
     8821 aattgggcat atgtttgcgc gcgtaaagaa ggcccgcaat acctatgggg atattgatta
     8881 aaaatatcca gtgccaggtt gccctggtga ccagcacgcc gccaagaacg gggcctaaaa
     8941 ttggccccac cagacccggc atggcgacaa aattcaatac aggaagaagt tcattacgag
     9001 gataagcacg cagtaaggcc agccgagcaa caggcatcat cattgcgccg cctatcccct
     9061 gaataacccg gaagacaacc agctgtggta gcgaattaga aagtgcgcag gccagagaac
     9121 ccaacgtgaa cagactcacg gcaagggtaa aaatgcgacg cgtaccgaag cgatcggcta
     9181 gccatccgct taccggaata aacatcgcca ccgtcagcgt ataactgatg atggctgatt
     9241 gcatcgcgag aggagaacga ttaaggctat gagcgattgc gggtaaggcg gtattcagaa
     9301 tagtggcatc aagtgcctgc atgaagaagg ccatcgccgc gatccacggc aaacccgcca
     9361 tactacgctt ctttttatcg ctcattcaat gtcctgttat cgggttatca cttatcaggt
     9421 gagcgtagca gcgcctgaca agttttaaat gccgcgtcgc catcgctttg gataatcgca
     9481 tcgacaatcg cctggtgcag atccagcttt atcactgtgt cgctggtaat tgacgtgaag
     9541 taagtgtgat aaaccgaatg gaatagcgag gcgaatgagg tcaaaaacgg attggcgctc
     9601 atttcataga tatgctcatg ccaggccata tcgacttcga tccagcgttc acggcgaaag
     9661 ttctttttta atgccgccat ttcggccatt aacgtattga gatgcgcctt ctgttccgcg
     9721 gtgccaaccg ttgctgccag taggcaggct tgcggctcca gacagatacg cataaccaga
     9781 aagtgatcga tgacctgatg aaagttctct tctgtcatcc accaggtaag caattcctga
     9841 tcaagaaaat tccagtttga ttgtggcatg acccgggtac caattcgcgg tcgcggtaaa
     9901 accatccctt ttgccgttaa cgttttgacc gcttcgcgta ccgctgtacg actcactcca
     9961 aattgctcgc ccagctcaat ctcaccaggc aaaatggtgc cgggttcata ttcacctttt
    10021 aagatgcgtt gcgccagctt ctcagccaga acatacgaaa ggtttttctg tgcagctaac
    10081 tgttgtgcgc ttaaaggcat tactaatctt cctttttctt tttattcctc cttagtatgc
    10141 caccaggaag tgtgattacg gttgcaaaaa cggcaaattg cttgttttat ggcacattaa
    10201 cggggctttt gct
//
LOCUS       DAJTNN010000146        10205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTNN010000146 DAJTNN010000000
VERSION     DAJTNN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.146"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OO150_003219"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OO150_003219"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5517263.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="OO150_003220"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="OO150_003220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5517264.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="OO150_003221"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="OO150_003221"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5517265.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="OO150_003222"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="OO150_003222"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5517266.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHLEQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3010..4599
                     /gene="prfC"
                     /locus_tag="OO150_003223"
     CDS             3010..4599
                     /gene="prfC"
                     /locus_tag="OO150_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5517267.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4992..5597
                     /gene="osmY"
                     /locus_tag="OO150_003224"
                     /pseudo
     CDS             4992..5597
                     /gene="osmY"
                     /locus_tag="OO150_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
     gene            5724..5885
                     /locus_tag="OO150_003225"
     CDS             5724..5885
                     /locus_tag="OO150_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5517268.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6007..7080
                     /locus_tag="OO150_003226"
     CDS             6007..7080
                     /locus_tag="OO150_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5517269.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGCYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            7077..7859
                     /locus_tag="OO150_003227"
     CDS             7077..7859
                     /locus_tag="OO150_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5517270.1"
                     /translation="MICRFIDTHCHFDFLPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(8073..8936)
                     /locus_tag="OO150_003228"
     CDS             complement(8073..8936)
                     /locus_tag="OO150_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5517271.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8908..>10205)
                     /locus_tag="OO150_003229"
     CDS             complement(8908..>10205)
                     /locus_tag="OO150_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5517272.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRILTQDEIDVRIKRFWRYLDGTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEIPPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
BASE COUNT         2410 a         2666 c         2749 g         2380 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt tacgccgcat
      241 ccagcaaggc ttgcggcggg atacttaacc ttttttcgcc tggcgggtca taatggcgcg
      301 atataccttg aaacgcccgg tttgcgcaat cacttcatgg aagccaaatg tttcatccag
      361 cacgtccgga taaggcagga aggcgttcgc tacaattcgc agctcgccgc cactattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacacagtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acccccgcgc cacagccgac atccagcact ttacctttcg tgtgcggagt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat cggcatcaaa
      841 taccggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatctg tccccactgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc gtatcgcaat ctgcgacgtc
     1081 atccaccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacag atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa aactttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgtg gtcgatcctg actatcagcg tcagggattg ggaagggtgc tgctgaaaca
     2041 tctgatcgac gaactggaaa aacgcggcgt ggcgacacta tggctggaag tccgtgcttc
     2101 aaacgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gtttcgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgctaaaatc
     2581 ggcatcatca ccaacggttt tagcgccttg cagcaagtgc gtctggaacg gacgggcctg
     2641 cgtgattact ttgatttgct ggtgatttcc gaagaagttg gcattgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaactggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacttgc
     2821 tggctgaatg cgcaccatct cgagcaacca gaaggcatcg cgcccacctg gaccgtttca
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc acccggacgc cggtaaaact accatcaccg agaaggtgct gctgttcgga
     3121 caggccattc agaccgctgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg
     3181 gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt
     3241 ccgtatcacg attgcctggt taacctgcta gacaccccgg ggcacgaaga cttctcggaa
     3301 gatacctatc gtaccctgac agcggtggac tgctgcctga tggttatcga cgctgcaaaa
     3361 ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc
     3421 ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact gctcgatgaa
     3481 gttgagaacg agctgaaaat cggctgcgca ccgatcacct ggccgattgg ctgcggcaag
     3541 ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctttatca gagcggtaaa
     3601 ggccacacga ttcaggaagt ccgtattgtt aaagggctga ataacccgga tctcgacgct
     3661 gcggttggtg aagatctggc acagcagcta cgtgacgaac tggaactggt gaaaggcgcg
     3721 tctaacgagt tcgacaaaga actgttcctt gcgggcgaaa tcactccggt attcttcggt
     3781 actgcgctgg gtaacttcgg cgttgatcat atgctggatg gcctggtgga gtgggcccct
     3841 gcgccgatgc cgcgtcagac tgatacccgt accgtagagg cgagcgaaga caaatttacc
     3901 ggcttcgtat ttaaaattca ggccaatatg gacccgaaac atcgcgaccg cgtggcgttt
     3961 atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg
     4021 aaagatgtgg ttatctccga cgcgctgacc tttatggcgg gcgaccgttc gcacgttgaa
     4081 gaagcgtatc cgggcgatat cctcggcctg cacaaccacg gcactattca gatcggcgac
     4141 acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg
     4201 ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta
     4261 cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc
     4321 gttggtgctg ttggtgtgct gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac
     4381 aacgttgaag cagtatatga atcagtcaac gttgccactg cccgctgggt agaatgtgcg
     4441 gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc
     4501 ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt
     4561 tatcccgacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc agggcgcggt
     4621 agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg ttcttaaaac
     4681 attcactttt gcttatgttt tcgctgatat cacgagcggt ttcaaaattg tgatctatat
     4741 ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta gatatcagta
     4801 tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg ctgtaatggt
     4861 cgttattcat cacccatcgc ttttgtgatg gcggccattg acttctgtag agggtgaagt
     4921 ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag caaacataca
     4981 ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg gctgtaatgt
     5041 tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag actaccaatg
     5101 aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc atggatgaca
     5161 gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc aagagcaccg
     5221 atatctctgt caaaaccgag caaaaagtcg tgaccctgag cggtttcgtt gaaagctagg
     5281 ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc tctgtcagcg
     5341 acaaactgca cgttcgcgac gctaaagaag gctcggtgaa gggctacgcg ggtgacaccg
     5401 ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct tcccgtcatg
     5461 tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtagat tctcaggcac
     5521 aaagtgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg
     5581 atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat
     5641 gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa gcggtgacat
     5701 taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg gttatcgcgt
     5761 taatcgccgc cgcacttggg tttggtggtt tggccggtac cgctgcaggc gcagctaaaa
     5821 ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc cgaaaacgac
     5881 cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga ctggcttttg
     5941 cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa aaacaggaag
     6001 gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg
     6061 ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg tggaattttc
     6121 acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga tctttatctc
     6181 ggcacatctg ccggggcgca gaacctctcg gcgtttatct gcaatcagcc cggttacgcg
     6241 cgtaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc
     6301 cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag ccagatgccg
     6361 ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta tatgtgcgcc
     6421 tgtcgtcagg atgactacgc gcctaattac tttttaccaa ccaaacaaaa ctggctggat
     6481 gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc gctggaaggc
     6541 attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag
     6601 ggcgctaaaa cgttggtcgt cattcgcact gtgccgtcac aaatgtacta cacgccgcag
     6661 tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct ggtcaatctg
     6721 gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa accaccgggc
     6781 aagctgcgga tattcgaaat ttatccgccg aagccattac atagtatcgc gcttggcagt
     6841 cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggttg ttatttcctc
     6901 gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct ggtgccagtg
     6961 gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac gctggttgcc
     7021 gaagtgattg acgctccgca ggcgaacgac ccgacattta acaatgagga tctggcttga
     7081 tttgccgttt tatcgacacc cactgccatt ttgatttcct gccgtttagt ggcgatgaag
     7141 aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag
     7201 aggcggaaaa ttttgcccgt gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg
     7261 cattgggctt gcatccaggt atgttggaaa aacatagcga tgtgtctctt gagcagctac
     7321 agcaggcgct ggaaaggcgt ccggcgaagg tggtggcagt gggggaggtc ggtctggatc
     7381 tcttcggcga cgatccgcaa cttgagaggc agcagtggtt actcgacgaa cagctgaaac
     7441 tggcgaaacg ctacgatctg ccggtgatcc tgcattcacg gcgcacacac gacaaactgg
     7501 cgatgcatct taaacgccac gatttatcgc gcactggcgt ggttcacggt ttttccggca
     7561 gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta ggcggtacta
     7621 tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt
     7681 tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg cagcctaacc
     7741 gaccggaaca ggctgtgcgg gtgtttgacg tgctttgcga gttgcgcccg gaaccagaag
     7801 atgagattgc cgaagtgttg cttaataata cgtatgcgtt gtttagcgtt agtgggtagt
     7861 ttacccttcg taggcagtat ccggcagtcg gcgcataatg cctgatgcga cgcttgtcgc
     7921 gtcttatcat gcctacaggt ttgtgccgaa ccgtaggccg gataaggcgt tcacgccgca
     7981 tccggcagtt ggcgcacaat gcctgatgcg acgcttgtcg cgtcttatca tgcctacaag
     8041 tctgtgccga accgtaggcc gtatccggca tgtcacaaat agagcgccgg aaatatcaac
     8101 cggctcaccc cgcgcacctt taacgcatca gccaacggct caacgtcttc cggcgtggcg
     8161 ctcgcccagc tttgcgcctc gccatacacg ccgtgggcat gaaacgcatt caggcgaacc
     8221 ggaacatcgc caagtctctt gataaacgtc gccagctcat cgatgtgatg caaataatcc
     8281 acctggtctg gaattaccag caaacgcagt tccgccagct tgccgcgctc tgcaagcaaa
     8341 cagatgctgc gcttaatctg ctgattatcg cgtccggtta agtgttgatg acattcgctc
     8401 ccccacgctt tgagatcgag cattgcgccg tcacacaccg ggagtaattt ttcccagccg
     8461 gtttcgctca acatgccgtt actgtccacc aggcaggtga ggtggcgcag ttgcggatcg
     8521 tttttgatag cagtaaacag cgccactaca aacggcagtt gggtcgtggc ttcgccgccg
     8581 cttaccgtta tcccttcaat aaacagcacc gctttgcgga catggctaag cacttcgtcc
     8641 acgctcatgg attgcgccat tggcgtggca tgttgcggac acatcttcag gcaggtatca
     8701 cactgctcgc agaccacagc gttccacagc actttgccgt caacaatctg caacgcctga
     8761 tgcggacact gtggcacgca ctctccgcaa tcattgcagc gtcccatcgt ccacggattg
     8821 tgacagtttt tgcagcgcag attgcagccc tgcagaaaca gagccagacg actgcctggc
     8881 ccgtcaacgc aggagaaggg gataatctta ctgactaaag cgcatctgct gttcatggct
     8941 tatcacgcgc ggctggcgtt ccagaatacg agtgttgcgt gtggcttctt cgcccagcca
     9001 ggtggtgttg gtgcgtgaac cttcggcgcg atatttttct aaatccgaca aacgcaccat
     9061 ataaccggta acgcgaacca gatcgttacc gctgacattg gcggtaaatt cacgcattcc
     9121 ggctttaaag gcaccgaggc aaagctgtac cagcgcctgc ggattacgtt tgatggtttc
     9181 gtcgagcgtc agaatgtcgc tgatgccgga ataataataa gcatgatgcg gggcgacagt
     9241 ttgcagatgg atgatcggat ctggctcatc gccatacggc aaacgcgcgc ccggcgtggt
     9301 gccgatatcg gaactgatcc ccgactgtgc gtgtaacatg gcgcgttttt gccagccata
     9361 tttcacgggg gtattggcga caaactccgc cagttgcgcg ctgatgcgat aacccacttc
     9421 atttgcggcg gcttctttac cgtagcgcgc ggcaatccct tctttctcac acagcaagtt
     9481 aaccgcttcc gccagcccat acatgccaaa cattggcaca aaacgttcag ggttaatcag
     9541 cccttctttc accaggaagc tattctcaaa gaagtgtgat tgttgataga ggaattcaca
     9601 ccgcgcatcg atgatggcga tctgctgctg gcagtagtgc ggtagagtgc gcgtaaagaa
     9661 gtcatccagc gattcgctgc gctcggcaat ggctttcagg ttaaggcgta ccagcgtgct
     9721 gccaccaccc gccagcggca gtgagttgta acagctcaca atcccgtagc ccccttttgt
     9781 gaaaatttta tcatgcaccg gaccgttggc gatgtgcggt ttgctacatt cacagatgtt
     9841 cttcgccact tccagcagca ggtcatcagg ggggatttca ggatcgtaga taaaggtcag
     9901 gttcggtgaa acctgcttca actctgcgtc tgcacgtaag atcgcgcggg taatgggcga
     9961 atcagacggg ccgatattgg cgtgcataaa ggcgtctggc agggttccgt cgaggtaacg
    10021 ccagaaacgt tttattcgaa catcgatctc gtcttgtgtt agaattctaa catacggttg
    10081 caacaacgca tccagttgcc ctaggtagac cggcatcgat gtgaccgacg gtacgtggtg
    10141 gtaaagaatg gtcagcagag agagtgcgtc atcaagatct ttcgcgcctt ccagctccag
    10201 ccatt
//
LOCUS       DAJTNN010000147        10152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTNN010000147 DAJTNN010000000
VERSION     DAJTNN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.147"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..610
                     /locus_tag="OO150_003230"
     CDS             <1..610
                     /locus_tag="OO150_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5517273.1"
                     /translation="ILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQMIAL
                     RIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDYSWI
                     VQPVANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTELGSRIICRDKQLIFE
                     EAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            607..1221
                     /locus_tag="OO150_003231"
     CDS             607..1221
                     /locus_tag="OO150_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR5517274.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1278..2735)
                     /gene="pepD"
                     /locus_tag="OO150_003232"
     CDS             complement(1278..2735)
                     /gene="pepD"
                     /locus_tag="OO150_003232"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5517275.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2996..3454
                     /gene="gpt"
                     /locus_tag="OO150_003233"
     CDS             2996..3454
                     /gene="gpt"
                     /locus_tag="OO150_003233"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5517276.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3546..4790
                     /gene="frsA"
                     /locus_tag="OO150_003234"
     CDS             3546..4790
                     /gene="frsA"
                     /locus_tag="OO150_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5517277.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4848..5249
                     /gene="crl"
                     /locus_tag="OO150_003235"
     CDS             4848..5249
                     /gene="crl"
                     /locus_tag="OO150_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5517278.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5288..6343)
                     /gene="phoE"
                     /locus_tag="OO150_003236"
     CDS             complement(5288..6343)
                     /gene="phoE"
                     /locus_tag="OO150_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5517279.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6631..7734
                     /gene="proB"
                     /locus_tag="OO150_003237"
     CDS             6631..7734
                     /gene="proB"
                     /locus_tag="OO150_003237"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5517280.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7746..8999
                     /gene="proA"
                     /locus_tag="OO150_003238"
     CDS             7746..8999
                     /gene="proA"
                     /locus_tag="OO150_003238"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5517281.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9114..9189
                     /locus_tag="OO150_003239"
     tRNA            9114..9189
                     /locus_tag="OO150_003239"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9147..9149,aa:Thr,seq:cgt)
     gene            complement(9204..>10152)
                     /locus_tag="OO150_003240"
     CDS             complement(9204..>10152)
                     /locus_tag="OO150_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5517282.1"
                     /translation="KKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHE
                     ENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIK
                     VPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRR
                     VAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRK
                     MRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRR
                     YAHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
BASE COUNT         2431 a         2499 c         2644 g         2578 t
ORIGIN      
        1 tattttacag cggcatattg cctcgttctc gcatcatggt ttgcctcaag gcagtgacga
       61 cgcgctacgg tatattgcgg aacatgacga tgcgctggcg tttgcgtgta ttaatcgcca
      121 gatgatcgct ttgcgcataa tgcaacaagt taaggccacg ggaagtccgg ttctggatgt
      181 ggcgcataac tttgttagcg cgtgtcaaat cggcgatcaa cagggctggt tgcatcgtaa
      241 aggtgccacg ccggatgaca acggtctggt gattattccc ggttcacgtg gtgattactc
      301 ctggattgta cagcctgttg cgaacgaaaa aacgctccac tcgctggcgc atggggcagg
      361 gcgtaaatgg gggcgcaccg agtgtaaatg gcgtctggca gcgaaataca cggtgacgca
      421 actctcgcgg actgaacttg gcagtcggat aatttgtcgc gataaacaac tcatctttga
      481 agaagcgcca caagcctata aatcggctga aagcgtggtg caatgtctgg tgcaggccgg
      541 tttaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg ctcaaaaaca gtggagggaa
      601 aaaaggatga tcttgctaca actctcctca gcgcaggggc cggaagaatg ttgtctcgca
      661 gtgaaaaagg cgctggacag gctaattaaa gaagctgccc gacaagacgt cgcggtaacg
      721 gtgttggaaa cagaaacggg ccgctactct gacacgctgc gttcagctct ggtttctctg
      781 gatggcgata acgcatgggc attaagcgaa agctggtgtg gcactattca gtggatttgt
      841 ccgagtccgt atcggcctca tcatgggcgc aaaaactggt ttctgggcat tgggcgtttt
      901 accgctgatg agcaggagca atccgatgcg atccggtatg agacgctgcg ttcgtcgggg
      961 ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac gcgccacgca tttggcatcc
     1021 ggtattagcg tgaaggttca gtcagagcgt agtcagcatg ctaacaagcg gctggcacga
     1081 ttgctgattg cctggaagct ggagcagcag caacaggaaa atagcgcggt gctgaaatcg
     1141 cagcggcgaa tgttccatca ccagattgaa cgtggcaacc cgagacggac atttacaggg
     1201 atggctttta tcgaaggata atgaaggatg aaactgccgg aaggcgatta aacgccatcc
     1261 ggcagcaaat caaataatta cttcgccgga atttctttca gcagttcagt cagcagtgtc
     1321 cagtaatgac ctacgctttc gatgtgaact tgctcatccg gagagtgtgg accggtgata
     1381 gttggcccga tagaaaccat gtccatttcc ggatacggtt ttttgaacag accacattcc
     1441 aggcccgcgt ggataatctg gatgttcggc gtcttgttga acaggcgctg ataggtttca
     1501 cgtaccagat gcatcaccgg agaattagcg tccggctgcc agccaggata tgcgcctttc
     1561 gcttcggttt tcgcgccagc cagtttaccc agcgaatcca gcatgctcac cacgtagtct
     1621 ttaccgctgt cgatcagtga acggatcagg cagtgaattt ctacgttatt gtcagtcatg
     1681 gtcaccacac cgacgttcag ggaggtttca accacacctt tggctacatc ggagttgcgg
     1741 atcacaccgt tcggggtggc gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc
     1801 agggcagctt tatcgttcgc tacagagtcc agcaacaagg ccagattttt ctctttctct
     1861 gccagctcgt ttttcaggat ctcctgatag gtattcacca gagatttcag tgcgtcgact
     1921 ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg
     1981 ccgccgttga aatcgataag gcgcaggtcc agttcttctg catgacccgc caggaagcgc
     2041 accagcagtt tgttggcatt acccaggcca acgtggattt caccgccgga gtgaccgcct
     2101 ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct
     2161 aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac ccatgtagat ttcaccttct
     2221 tcttcggagt cggtgttaat cagaatatca gcctgcaacc agttgctctg taagccaaaa
     2281 gcaccgtcca taccggcttc ttcggtcatg gtcagcagca cttccagcgg accgtgaacc
     2341 acgttttcgt cagccagaac cgccagtgca gaggccatac caatgccgtt atccgcaccc
     2401 agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat aaggctggat aggatctttc
     2461 gtgaagtcat gcacggtgtc gttatttttc tgcggcacca tatcgaggtg ggcctgtaag
     2521 acgaccggtt tacgattttc catacctgcg gtagcaggtt tacgaatcag gatattacct
     2581 acctgatcgc gttcgacatg gaaacctttc tcttttgccc aaccaacaat gtattcagcg
     2641 agttgctctt catgatagga cgggtgcgga atagaacaga ttttggcaaa aatatcccac
     2701 agcggctgtg gagataattg agacagttca gacacgttaa gtctccttgt cgatcacccg
     2761 caaaacagta ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa gacaggcttg
     2821 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     2881 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     2941 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     3001 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     3061 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc
     3121 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     3181 ctacgatcac gacaaccagc gagagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     3241 aggcttcata gttattgatg acctggtgga taccggtggt actgctgttg cgattcgtga
     3301 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     3361 tgatgactat gttgttgata ttccgcaaga tacctggatc gaacagccgt gggatatggg
     3421 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     3481 cgttgttctt tttaacttca ggcaggttac aatagtttcc agtaagtatt ctggaggctg
     3541 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     3601 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggctc
     3661 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     3721 gcggtattga cccacgtgaa atcctcgacg tccaggcacg tattgtgatg agcgatgctg
     3781 aacgtaccga cgatgattta tacgatactg tgattggcta ccgtggtggc aactggattt
     3841 atgagtgggc cacccaggcg atggtgtggc aacaaaaagc ctgtgcggaa gaagatccgc
     3901 aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta caacattgcc gcctatcctc
     3961 atctgaaagg agatgacctg gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag
     4021 aggccgccca gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta cccggcggtg
     4081 cgcccatcac cggctttttg catatgccga aaggcgatgg cccgttcccg acagtattaa
     4141 tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa cgttattttg
     4201 cgccgcgcgg cattgtgatg ctgactattg atatgccgtc ggtgggcttt tcttcaaaat
     4261 ggaagctcac ccaggactcc agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg
     4321 taccgtgggt ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg
     4381 ccgtgcgtct ggcatatctt gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg
     4441 tagttcatac cctgttgagt gattttaagt gccagcaaca ggtgccggaa atgtatcttg
     4501 acgttctggc gagtcgtttg gggatgcatg atgcttccga cgaagcgtta cgcgtggagc
     4561 tgaatcgcta ttcattaaaa gtgcaaggat tgctgggacg tcgctgccca acgccaatgt
     4621 tatcaggcta ctggaagaac gatccgttca gcccggaaga ggactcacgc ttaatcacct
     4681 catcatctgc tgacggtaaa ttattagaga tcccatttaa cccggtgtat cggaattttg
     4741 acaaaggtct tcaggaaatc accggctgga tcgaaaaacg cttgtgttaa aaatttgcta
     4801 aattttgcca atttggtaaa acagttgcat cacaacagga gatagcaatg acgttaccga
     4861 gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc actaggcccg tatattcgtg
     4921 aaggtaagtg cgaagataat cgattctttt tcgattgtct ggctgtatgc gtcaacgtga
     4981 aaccggcacc ggaagtgcgt gaattctggg gctggtggat ggagctggaa gcgcaggaat
     5041 cccgttttac ctacagctac cagtttggtc tgttcgataa agcaggcgac tggaagagtg
     5101 ttccggtaaa agatactgaa gtggttgaac gactggagca caccctgcgt gagtttcacg
     5161 agaagctgcg tgaactgctg acgacgctga atctgaagct ggaaccggcg gatgattttc
     5221 gtgacgagcc ggtgaagtta acggcgtgag tgaaatgtgc cggatgcatc acatccggca
     5281 atatctatta aaactgatac gtcatcccaa tcgcgacaat atcatcatta ttaatattca
     5341 atttgttatc gctatccagt tggttgattt tataatcaac aaatgctgac atatttttgt
     5401 tgaaataata cgtagcaccg acgtcgatat aattaaccag atcttcacca ccgatgccct
     5461 caatatcttt ccctttcgat aagacataac ccagcgatgg acgcagacca aagtcaaact
     5521 ggtattgagc gaccgcttca aagttctgtg tcttattggc aaagccgcca gttattggcg
     5581 tcattttgcg agtttcagaa tagaaagttg ccagataaat attattggca tcgtatttca
     5641 gacctgttgc ccatgcttct gcacgcttgc ctgtgccacg gctttgcagg ttctgctcgt
     5701 tggtgcgatc tgagttggta taggccccac taatggcgaa atcgctgccg ccaaagtcat
     5761 atgtcaatga cgtgccgaag ccatcgccgt tttgcttttt aacgtcgcgg ttttcgtttt
     5821 tcccttgata ttgcagggtt aagttcaggc catcgataac gccgaagaag tcggtgttcc
     5881 gatacgtcgc cagaccgctg gcgcgtttgg tcataaagtt gtcggtctgc gccgaggagt
     5941 cgccaccaaa ttccggaaac atatcggtcc aggcttccac gtcatacaac gcgcccaggt
     6001 tacgaccata atcgaaagaa cccaaatctt tatatttcaa cccggcaaaa gcgagacgcg
     6061 ttttttgctg tgcagtatca ctctctgctt tattaccggc aaactctgct tcccaacgac
     6121 cataaccagt cagttgatcg ttaatttgtg tttcgccttt aaaaccaaaa cggatataac
     6181 tctggtcgcc atctttactg tcgttatcac tcatataatg catggcttta actttgccat
     6241 agacatccag tttattaccg tctttgttat atatttctgc ggcctgtacg gatgcagatg
     6301 ccacaatgcc catcaccact aatgccagag tgctcttttt cattttcatt cctgatttta
     6361 attaacgcgc gaatattcag cgggagagcc ccgttgaaaa caggaaagtt tttaacctga
     6421 gattgttaaa gatatattac agattaataa tattcttaaa atgtggtaat ttattaaatc
     6481 tgtaataaaa gcgtaaacaa ctgccgctag gcttgctgat cccgcgcaac aaaacgccat
     6541 gctttgctcg cagatggttg gcaaccgacg acagtcctgc taaaacgttc gtttgatatc
     6601 atttttccta aaattgaatg gcagagaatc atgagtgaca gccagacgct ggtggtaaaa
     6661 ctcggcacca gtgtgctaac aggcggatcg cgccgtctga accgtgccca tatcgttgaa
     6721 cttgttcgcc agtgcgcgca gttacatgcc gccgggcatc ggattgttat tgtgacgtcg
     6781 ggcgcgatcg ccgccggacg tgagcacttg ggttacccgg aactgccagc gactatcgcc
     6841 tcgaaacaac tgctggcagc ggtagggcag agtcgactga ttcaactgtg ggaacagctg
     6901 ttttcgattt atggcattca cgtcgggcaa atgctgctga cccgtgctga tatggaagac
     6961 cgtgaacgct tcctgaacgc ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt
     7021 ccggtaatca atgagaacga tgctgtcgct acggcagaga ttaaagtcgg cgataacgat
     7081 aacctttctg cgctggcggc gattctggcg ggtgccgata aactgttgct gctgaccgat
     7141 caaaaaggtt tgtataccgc tgacccgcgc agcaatccgc aggcagaact gattaaagat
     7201 gtttacggca ttgatgacgc actgcgcgcg attgccggtg acagcgtttc aggcctcgga
     7261 actggcggca tgagtaccaa attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac
     7321 accattattg ccgcgggcag caagccgggc gttattggtg atgtgatgga aggcatttcc
     7381 gtcggtacgc tgttccatgc ccaggcgact ccgcttgaaa accgtaaacg ctggattttc
     7441 ggtgcgccgc cggcgggtga aatcacggta gatgaagggg caactgccgc cattctggaa
     7501 cgcggcagct ccctgttgcc gaaaggcatt aaaagcgtga ctggcaattt ctcgcgtggt
     7561 gaagtcatcc gcatttgcaa cctcgaaggt cgcgatatcg cccacggtgt cagtcgttac
     7621 aacagcgatg cattacgccg tattgccgga caccactcgc aagaaattga tgcaatactg
     7681 ggatatgaat acggcccggt tgccgttcac cgtgatgaca tgattacccg ttaaggagct
     7741 ggccgatgct ggaacaaatg ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac
     7801 tctccagccg cgaaaaaaat cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa
     7861 gcgaaatcat tctcaacgct aacgcccagg atgttgctga cgcgcgagcc aatggcctta
     7921 gcgaagcgat gcttgaccgt ctggcactga cgcccgcacg gctgaaaggc attgccgacg
     7981 atgtacgtca ggtgtgcaac ctcgccgatc cggtggggca ggtaatcgat ggcggcgtac
     8041 tggacagcgg cctgcgtctt gagcgtcgtc gcgtaccgct gggagttatt ggcgtgattt
     8101 atgaagcgcg cccgaacgtg acggttgatg tcgcttcgct gtgcctgaaa accggtaatg
     8161 cggtgatcct gcgcggtggc aaagaaacgt gtcgcactaa cgctgcaaca gtggcggtga
     8221 ttcaggacgc cctgaaatcc tgcggcttac cggcgggtgc cgtgcaggcg attgataatc
     8281 ctgaccgtgc gctggtcagt gaaatgctgc gtatggataa atacatcgac atgctgatcc
     8341 cgcgtggtgg cgctggtttg cataaactgt gccgtgaaca gtcgacaatc ccggtgatca
     8401 caggtggtat aggcgtatgc catatttacg ttgatgaaag tgtagagatc gctgaagcat
     8461 taaaagtgat cgtcaacgcg aaaactcagc gtccgagcac atgtaatacg gttgaaacgt
     8521 tgctggtgaa taaaaacatc gccgatagct tcctgcccgc attaagcaaa caaatggcgg
     8581 aaagcggcgt gacattacac gcagatgcag ctgcactggc gcagttgcag gcaggccctg
     8641 cgaaggtggt tgctgttaaa gccgaagagt atgacgatga gtttctgtca ttagatttga
     8701 acgtcaaaat cgtcagcgat cttgacgatg ccatcgccca tattcgtgaa cacggcacac
     8761 aacactccga tgcgatcctg acctgcgata tgcgcaacgc ccagcgtttt gttaacgaag
     8821 tggattcgtc cgctgtttac gttaacgcct ctacgcgttt taccgacggc ggccagtttg
     8881 gtctgggtgc ggaagtggcg gtaagcacac aaaaactcca cgcgcgtggc ccaatggggc
     8941 tggaagcact gaccacttac aagtggatcg gcattggtga ttacaccatt cgtgcgtaaa
     9001 taaaaccggg tgatgcaaaa gtagccattt gattcacaag gccattgacg catcgcccgg
     9061 ttagttttaa ccttgtccac cgtgattcac gttcgtgaac atgtcctttc agggccgata
     9121 tagctcagtt ggtagagcag cgcattcgta atgcgaaggt cgtaggttcg actcctatta
     9181 tcggcaccat ttaaatcaat aagttacaca tcattagtac gttccttatt tcttgactgg
     9241 gacaaatttg ggaccgatgg gttcagaatc gagtctattt gccgtgcgtg ttcggtaagg
     9301 tgattaggtg cgaggtgagc atatcgacga accatttcga tagactccca gcctcccatt
     9361 tcctgtaaca ctgataacgg gactccggct tgaaccagcc aacttgccca ggtgtgtctc
     9421 aagtcgtgaa atctgaaatc atcaatacct gctcgtctca gcgccgcttt ccaggctgtg
     9481 tttgcgtcat accgcatctt cctgactgtt ggcgctttcg ttccgtctgg tttggtacag
     9541 ctttccttat acacaaatac ccaacggtga tgattcccga tttgtttttt caatacgcga
     9601 catgcagtat cattcagcgc aacgccaatt gcgcggtttg atttactctc ttccgggttt
     9661 atccatgcca cccggcgctg catgtctatt tgttgccatt caaggttgat gatgttcgag
     9721 cgtcttaagc ctgttgcaag tgcaaattca acaacagact ttaatggctc cggacattca
     9781 tcaatcagcc tttgtgcttc atgaggctcc agccagcgga tccgtttatt ctttggttga
     9841 ggcactttaa taattggtgc cttatccagc attttccatt cacgctctgc ggctcttagc
     9901 agggccttta taaatgaaag atgcgtagcc ttcgttgcaa cggacgctgg ttttggcgtg
     9961 tattctggaa caggtttccc tttttttctg catgcttctg ccctgagttt ccagttttcc
    10021 tcatgacgcc ggttcgtcat tttctgcatt gctgaataaa tttttgattc agtaatgtct
    10081 cttagttgca ttcctgcgaa atgttgaagc cagaatccga tccggctttt gtcatcgtcc
    10141 agtgattttt ta
//
LOCUS       DAJTNN010000148        10137 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTNN010000148 DAJTNN010000000
VERSION     DAJTNN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.148"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..237
                     /locus_tag="OO150_003241"
     CDS             <1..237
                     /locus_tag="OO150_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5517283.1"
                     /translation="ETLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDML
                     VYEKTYTAELSSIAGTPIEAGPFDTVVLFKINYN"
     gene            389..1435
                     /locus_tag="OO150_003242"
     CDS             389..1435
                     /locus_tag="OO150_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5517284.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTCM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPDGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(1432..2223)
                     /gene="nei"
                     /locus_tag="OO150_003243"
     CDS             complement(1432..2223)
                     /gene="nei"
                     /locus_tag="OO150_003243"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5517285.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2259..2993)
                     /gene="pxpA"
                     /locus_tag="OO150_003244"
     CDS             complement(2259..2993)
                     /gene="pxpA"
                     /locus_tag="OO150_003244"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5517286.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(2983..3915)
                     /gene="pxpC"
                     /locus_tag="OO150_003245"
     CDS             complement(2983..3915)
                     /gene="pxpC"
                     /locus_tag="OO150_003245"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR5517287.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3909..4565)
                     /gene="pxpB"
                     /locus_tag="OO150_003246"
     CDS             complement(3909..4565)
                     /gene="pxpB"
                     /locus_tag="OO150_003246"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5517288.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIELLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4588..5331)
                     /gene="ybgI"
                     /locus_tag="OO150_003247"
     CDS             complement(4588..5331)
                     /gene="ybgI"
                     /locus_tag="OO150_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5517289.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5602..7083
                     /gene="dtpD"
                     /locus_tag="OO150_003248"
     CDS             5602..7083
                     /gene="dtpD"
                     /locus_tag="OO150_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5517290.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7125..8543)
                     /gene="phrB"
                     /locus_tag="OO150_003249"
     CDS             complement(7125..8543)
                     /gene="phrB"
                     /locus_tag="OO150_003249"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5517291.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACSNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8540..9049)
                     /locus_tag="OO150_003250"
     CDS             complement(8540..9049)
                     /locus_tag="OO150_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5517292.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9334..9708)
                     /locus_tag="OO150_003251"
     CDS             complement(9334..9708)
                     /locus_tag="OO150_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517293.1"
                     /translation="MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKY
                     ENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLIFMADFGESRYFKPAKI
                     AMNKNNLIIRTSDVEIAFKITK"
     gene            complement(9693..>10137)
                     /locus_tag="OO150_003252"
     CDS             complement(9693..>10137)
                     /locus_tag="OO150_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5517294.1"
                     /translation="IDKPAPVFNRDLNSDPYYLAVNNCYSYALNRYGNPGSRIFGGGG
                     LQPGELSGKEFSKLTCGSIFEASKNDGAKDLDNGSCPSGYHKAQLFIRPHNFIGMGGD
                     YHWYRQDANGEWSDKQGVGAIRFRGKDPFPPIDYPEKCGTIFLPN"
BASE COUNT         2361 a         2750 c         2438 g         2588 t
ORIGIN      
        1 gaaacgttgg ttgaagaaga taaagcattg ttgattggta atggtctgaa gctacgtttg
       61 ctggatgaaa acgcctcgcc ttataccttc aataaatatg ctgaatacgc cgatttcacc
      121 agcgacatgt tggtctacga aaaaacctat acagctgaac tttcgtccat tgcaggtacc
      181 cccatcgagg ctgggccctt cgatacagtg gtgcttttta aaattaacta taactgattt
      241 gccatgatgc cctttgcagc cggaaagggc atctctttca gaaaattgct gattttcctc
      301 ttttccttga ttactgttat tttttcctgg cccggctttc tttatgctgt atcgatacac
      361 caaagagaaa ataatgaggg agcgaaggat gccagttttg cagtggggaa tgttatgtgt
      421 gctatcactt ctcctttcta ttggtttcct cgcggtacat ctcccggcgg cgctattact
      481 tgggccgatg atcgccggga tcatcttcag tatgcgcgga attactctgc aacttccccg
      541 ctccgctttt ctcgccgcgc aggccattct tggctgcatg attgcgcaaa acctcactgg
      601 ttcgattctc accacgctgg ccgccaactg gccgatcgtg cttgctattt tactggtgac
      661 gctgctttcc agcgccatcg tgggttggtt attggtgcgc tatagctcac tgcctggaaa
      721 taccggtgcc tggggttctt ctccaggcgg cgcagcggca atggtcgcta tggcgcaaga
      781 ttacggcgca gatattcgcc tggtggcgtt tatgcaatat ctgcgagtgc tgttcgtcgc
      841 gggggccgct gttctggtca cctgcatgat gctgggtgat aacgctgaag cggttaacca
      901 gcaaatcgtc tggttcccgc cagtgagcat taatctcctg cttacgattt tgctcgcagt
      961 cgttgccggt acggcgggat gcatgctgcg tcttccttcc ggcacgatgc tcatcccgat
     1021 gctggcgggc gcagtgctcc agtctggtca gctcatcacc atcgaactac cggaatggct
     1081 gctggcgatg gcgtatatgg caattggctg gcggattggt cttggtttcg ataaacaaat
     1141 cttactgcgg gcactacgcc cactaccgca aatcctgctg tcgatttttg ctctgctggc
     1201 tatttgtgca ggtatggcgt ggggactgac ccggtttatg catattgatt ttatgactgc
     1261 ctacctcgcc accagtcccg acgggcttga taccgtggcg gtcatcgccg cagggagcaa
     1321 tgccgatatg gcgcttatta tggcgatgca aaccctgcgc ctgttcagta ttttgctgac
     1381 ggggccagcc attgcgcggt ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg
     1441 gcagcctgga caccagtaaa acgggcgcga ggacaacgtg gttttctcaa tgatgccgcc
     1501 acagcgttcg cacagttcgc catcacgatg aaaaacctta aagcgaaaca gcgccccatg
     1561 atgcttattc tcatccacct gcccccgcgt agcgtaagaa aaccgaggaa tctccagtaa
     1621 cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct ttcgctttat gatttccagt
     1681 caacccaacc tgccagagga tctccacccg caaataattg ccaagcccag ccagaaacgc
     1741 ctgatcgagc agtaatcctg caaactgacg gttacgaaag cgcggtgaca ataaccgttc
     1801 ctttaccacc tccggcgtca gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa
     1861 cggatgcgtg gtcagttgtt ccggcctcaa catctcaata tccgaggcgc tataaagcag
     1921 aatggttttg tcagccgttt gcagttttac ccgcaatact cgcgtggtct gcggctcttc
     1981 gccggtatca accacgcgcc agacgccgta aagctgatta tggctgtaga gcgttaagtc
     2041 gttggaaaaa tgagttaaca acgccttacc acgcgttccc acatgggtaa cgtgttgacc
     2101 gataagttgt gattgataag tttttaactg cgggaaggca aaccagacat cagttagtgg
     2161 tttgcctttg atcgccgcct ccaggttatc cgctgcacgg cggatctccg ggccttcagg
     2221 catggtgata tccttttttc attctttatt aatcggggtt atgctgcgac aacaatctcc
     2281 ttttcggcaa atgtagagcg tagtcggcgg gcgaacgcca gtgcgtgctc gccgtcgcca
     2341 tgcaggcaga ctgtttgcgc cgtgaccgtt gcccattcgc cggtgatact tttgactctg
     2401 ccgtgttgca ccatctctag cgtttgcgcc agcgcctgtt cttcgttttc aatcaacgcg
     2461 cctggctgac ttcgcggcac cagcgagccg tcagcctgat aaccgcgatc ggcaaacact
     2521 tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa tcagctcgct tcctgccagc
     2581 ccaacgagaa ccaatgctgg atcgcaagcg tataccgctc tggcgatggc gtctgccagt
     2641 tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg gtttgacatg acgcattacg
     2701 ccgccttgcg cacgggcaat cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa
     2761 acggtttctg gcggcagctg catggcgctg cgaccaaaat tttccctgtc gggaaaactc
     2821 gggtgagcgc caatcgcgac accatttttt attgcttcac gcacgcaagc ctgcatggtt
     2881 tgggcatcgc ctgcatgaaa tccacaagca atattggcag aggaaaccag cgttaatagc
     2941 tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca ggtcaatttt cattgtgtag
     3001 ccgccacgct aattgttcga aataacgttg ttgatcttgc cgcgctttca gtgcctcttc
     3061 cagtgaacac tggacaaaat gaatcggctg accgagcgga atttgcgcca gatggtacat
     3121 atcagcctca atgatacagg caatacgcgg gtaaccaccg gtggtctgtg cgtcgttcat
     3181 caacacaatc ggctgcccgt tatgcggcac ctgcaccaca cccggtaaca aaccgtgaga
     3241 taacagttcg cgatcggtgg tgcgttttaa aatttgcccc tgtaagcgat agcccatgcg
     3301 gttactttgc gggctaagct gccagggcga acgccagaat gcatcctgcg aggcgcgatc
     3361 gaactcatga tattccggcc ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt
     3421 aacgccctgc gcttccataa aatcacgctt cgatttgcca atcgggagtc ggtcaccatc
     3481 cttcagtaaa cggccttcca gcccgccaat ccccacgttg agatcggtgc tgcatgaccc
     3541 cattaccggc ggaacatcaa taccacccgc gaccgccaga taactgcgca tgccgtgctg
     3601 cgggcgttta agcgttaaac gctggcctgc tttcatcggc aagcgccagc cggtccagac
     3661 ggcgttatca tccagccgcg cttcgcaacc ggcacccgtc agagcaaacc acccatcagt
     3721 ttcgaactca accgttaact gaccgagcgt gatctccagc gcgggggcat tggcgtcatt
     3781 acccaccagc aggttagcaa tgcgtaacgc aggcatatcc agtgcgccgc agtggctgat
     3841 acccgactgg cgaaaaccgt gacgaccgcc atcctgcaca gtggtataca tgcccgcacg
     3901 aataatcttc agcatactcc ctccttctgc ggtacaaagc gcacgctgtc tcccggacgt
     3961 aataagatgg gttcgtcacg cgccggatca aacaggctga gtgaggtatg accaatcaac
     4021 tgccagccac ccggcgttgc cagcggataa acaccagtct gcggcccgcc gatcccgaca
     4081 gaacctgccg gaacgagcaa gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc
     4141 aaactcccga gatacgggaa gcccggttga aaacctaaaa accagaccac gtattccacg
     4201 gaggagtgca attcaacaac ctgtttttcg ctcaacccgc aatgcgccgc gaccaccgcc
     4261 aaatccggtc cgcctgcacc accgtaaacc accggaattt caataaagcg agactcaggt
     4321 tccagggcct cgctctcctc ccaccagcgt tgcaaaagct ctatggcatc cagcgccagc
     4381 gactcaggat tacgcaaaat caccgtgata ttgttcatgc cgggaatggc ttcaaccaca
     4441 ttcggcatat ccaccagacg ctgcgccagt cgccagatcc gtttctggct agccagcgtc
     4501 accggcggtt ccagttccag cactaccgcc gtttcaccta tcagataaca acgcgctcgt
     4561 tgcactttcg atccctctga ttattcgtta tgcaggatta ggaatatcaa taaaggtcac
     4621 atcaagatcg gtattttcat tcagccactc gctcaatgcg cgaataccac cacgttcagt
     4681 ggcatggtga cctgcagcat aaaaatgcaa tccctgctcg cgggctgaat gaatggtctg
     4741 ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa
     4801 actttgcccg ccgcccgtgc accaggcgac gcgctggacc acctcaggtc cggtatcgcc
     4861 acaccataac ggcttacgtc ccagacgcgc ttcaatccag gaggctagtt ccagtcccgg
     4921 cacaggcatg gtcagctctc cccacggcac caacggctca atttcgccca tgaccgtgat
     4981 ccccagtaac gccgccagct gtgcgttatt gcccagctca ggatgtgcgt caagcggcaa
     5041 atgccagcca tacaggttga tatcattcgc cagcaacgtt tttaaacggt tacgcttcat
     5101 tccgcgaatg accggagact ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc
     5161 cgcgcccaga cgcactgcct catcgagcaa agcctggctg gcggtgacac cggtaacaat
     5221 tttttgcacc gtctctttgc cttccacctg caaaccattc ggcgcgtaat cactaatcgc
     5281 cgcgctgttc agtttttcgt taatcagttg ttccagttcg gtgtttttca tcttcgcgcc
     5341 ttccgtattc tgatagctac accgttaaca tagcgttacc tcgcctgttc gtcgatagcc
     5401 aatcttttga catattaaga aacaaacccg acgccaaaag acgcaaattg tttatatcat
     5461 cacgcttcag ctaatgtata tttatgcaat cagacagcat attgcataaa taatcagatc
     5521 tgtatcaaag ttttaacttt aaggaaacac aagcacgccg cgcaactatt acgtgacgtt
     5581 gccgtcaatt gtggaattat tatgaataaa cacgcatcac agccgcgcgc tatttactat
     5641 gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg gcatgcgtgc cctgctgatt
     5701 ctctatctca ccaatcaact aaaatacaac gatactcacg cctacgagtt atttagcgcc
     5761 tactgttcgc tggtgtatgt cacgccaatc ctcggtggct ttttggcgga taaagttctc
     5821 ggcaatcgca tggcggtgat gctgggggcg ttattgatgg cgatcggtca tgtggtgttg
     5881 ggtgccagtg agatccatcc gtcattcctc tatctgtccc tggcgataat tgtttgtggc
     5941 tatggtctgt ttaaatccaa tgtcagttgc ctgctcggcg agctgtatga gccaaccgat
     6001 ccgcgtcgtg atggcggttt ctcgctgatg tatgcagcgg gtaacgtggg gtctattatc
     6061 gcacctattg cctgtggcta cgcccaggaa gagtacagct gggcgatggg ctttggcctg
     6121 gcggcggtgg gtatgatcgc gggtctggtc attttcttat gtggcaatcg tcatttcaca
     6181 catacccgcg gcgttaataa aaaagtactg cgtgcgacaa actttctcct gcctaactgg
     6241 ggatggctgc tggttctgct ggtggcaacg cctgcgctga ttaccgtatt gttctggaaa
     6301 gagtggtcgg tatacgcctt aattgtcgcg actattattg gcctgggtgt actggcaaaa
     6361 atttatcgca aagcagaaaa ccagaaacag cgtaaggagc tggggctgat tgtgacgctc
     6421 accttcttca gtatgttgtt ctgggccttc gcacaacagg gcggcagctc gattagcctt
     6481 tatatcgacc gcttcgttaa ccgcgatatg tttggttata ccgttccgac tgctatgttc
     6541 cagtcgatta atgccttcgc agttatgctg tgcggtgtgt tcctggcgtg ggtggttaaa
     6601 gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa aatttgctct tggtctcggc
     6661 ctgatgagcg ccgggttctg cattctgacc ttaagcgccc gctggtccgc aatgtatggt
     6721 cactcttctc tgccactgat ggtattaggc ctggcggtga tgggctttgc ggaactgttt
     6781 atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa tccccggtgt gaccggcgta
     6841 ttaaccggca tttatatgct gctttccggc gcgattgcga actatctcgc gggcgtgatt
     6901 gccgatcaga catcgcaggc ttcgtttgat gcttccgggg cgatcaacta ctccatcaat
     6961 gcatatattg aagtatttga tcaaattacc tggggcgcac tggcgtgtgt aggactggta
     7021 ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc gcgcgctggc gctggagtca
     7081 taataggcca gcaccatacc tgataaaacg ctcgctgcgt tttatcaggc tcctttcctc
     7141 gcttcttcgt acgctgccag cgttcgcagc cttgcctctt tgtgatcaac tatcggttgc
     7201 ggataatcca gcatcacacc tgctttctgc gcccacttcc acggctcatg caccgctttc
     7261 cctggtacat cgcgcagctc cggtaaccat cgacgaataa actcgccctc acggtcaaat
     7321 ttctcgccct gggttgtcgg gttgaaaata cgaaaatacg gcgctgcatc ggttccggtt
     7381 gaagcggccc actgccagcc accgttattg gctgccaaat caccatcaat cagctgcgac
     7441 atgaaatatc gctcgccttc gcgccagtcg atcaacaaat ctttcaccag aaaactggct
     7501 gtaatcatcc gtagccggtt atgcatccag ccagtgctgt taagctgacg catggcagca
     7561 tcgacaatcg ggtatcccgt tttgccttcc tgccaggcct gtaaatgtgc gggattgctc
     7621 tgccactgca cacgatccgt ccaggcaata aacggacaat gtttacacaa cgaggggtaa
     7681 tacgtcatca gatgacggta gaactcgcgc cagatcagct cactaagcca gacactaccg
     7741 gccccaccgt ccagcgcctg cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc
     7801 gataacccgc ccgttgccag gctggcggac aaacgactgg tgccttccac tgccggaaaa
     7861 tctcgttgtt gctcatattc tccggcaccg ttctggcaaa actggcgtaa ttgcgcaatc
     7921 gccgcttttt cttccaccgg gaaatgcgca gtatcgaaag actgacgagg ataattcagc
     7981 gtaatggatg gcgagggctc tatcgatccg ctactacgaa cttttggcgc agcgacgcac
     8041 tccggcatcc cttcccgcag ccgtttcagc caggcattct taaaaggcgt aaagactttg
     8101 tacatctcgt gattaccggt catcaccgcg ccaggcggca ggatcacgct gtcatcaaat
     8161 ccttcacaca ccacgttacg cagcgctctt tcaacttcca catcccgcgc ccgctcattc
     8221 acttcatact gatagttata aaacaggtgg gtaacgctgt tttccgcgca cacctgttta
     8281 actatttcga cactggcgac aaagtcatcc acttcacgga acaataaagg aatacctttt
     8341 tccgcaagcg ctatttgtag cccattcagt tgagcattga taagttcagc ctgacgcggc
     8401 gacatgttat gcgtcgccca ctggcgtggt gtagcgatat acaacgccag cacgcgtgca
     8461 gacgaattgc tgcaggcggc agccagtgcg agattatcgt gcaaacgtaa atcctggcga
     8521 aaccagacca gatgggtagt cataaaactc ctgaatggcg caagttaatc acacgcggcc
     8581 aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt
     8641 tgatacgcat cagcggcgca agaagtggtt gctcgccacg gcgataactg tcgataagcg
     8701 cagccagctg ctggcgttct gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca
     8761 tcaaaacatt ggtgtgatcg cgcgggttgg cggggtgaga aagcaaaaca actacgcgtt
     8821 ggcggtactg gttgtaatag tcatccagat tttgccactg gtgaatatca gccacgaacg
     8881 ggccaagttt gcggtactcc ggctgagaat gcgccagaaa gacgagttta tagcggctgt
     8941 gataatcgag aagcgccccg cgcgttaagc catgttcttt cagtcggtgt aactcatgaa
     9001 gggcaaagat acggattagg gtgctgtcat caaatcgttg tagattcatt actgctcctg
     9061 aacggtataa aggagtaagt gtagacgatg gtaaatgaat tttatggggc agagtttttg
     9121 cggggaagta ttttgttggt aaaaatatta tcaacttcat ctgagcgaga attattctac
     9181 tatttcccga gctgcattca ttttttgtat tttttatttt tagaaaataa cagttctcaa
     9241 agcttaatta cctcactatt aattgtattt caaactaaca atcgcactcc attttctctc
     9301 tttaaaaaac ataaacactg ttaaaacaaa tatttatttc gttatcttaa atgctatttc
     9361 tacatcactc gtccttataa tcaaattatt cttattcata gctatttttg caggtttaaa
     9421 atagcggctt tcaccaaagt cagccatgaa aattagacta caagttctcc cagcctttgc
     9481 acatgagaaa aaccacccat caactctatt tattcctgaa cctttaacac cataataaat
     9541 cagaatttct gggtattgtt catccttcac gttttcatat ttttggtatt taattgaaaa
     9601 ccaagagtct atactgccaa catcttcaaa aaatattgga gctgatgttt ccgcatgaac
     9661 atatgcagaa aatgctaaca aaatggcaat acttagtttg gtaaaaatat cgttccacac
     9721 ttttcaggat aatctattgg ggggaaggga tcttttcctc taaatctaat tgctcccact
     9781 ccttgtttgt cagaccattc gccatttgca tcctgacgat accagtgata atcccctccc
     9841 atgcctataa aattgtgagg acgaataaac aattgagctt tatggtaacc agatggacaa
     9901 ctcccattat ctaaatcctt ggcaccatca ttttttgacg cttcaaaaat actaccgcaa
     9961 gtgagtttag aaaactcttt tccagataat tctccaggtt gtaatcctcc gccgccaaaa
    10021 atcctactcc cagggtttcc atacctattt agagcatatg aataacaatt atttacggca
    10081 agataatacg gatctgaatt taggtctcga ttgaatactg gagcaggttt atcaatt
//
LOCUS       DAJTNN010000149        10096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTNN010000149 DAJTNN010000000
VERSION     DAJTNN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.149"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            121..987
                     /gene="folD"
                     /locus_tag="OO150_003253"
     CDS             121..987
                     /gene="folD"
                     /locus_tag="OO150_003253"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5517295.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQACIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            989..1201
                     /gene="ybcJ"
                     /locus_tag="OO150_003254"
     CDS             989..1201
                     /gene="ybcJ"
                     /locus_tag="OO150_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5517296.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1309..1830
                     /locus_tag="OO150_003255"
     CDS             1309..1830
                     /locus_tag="OO150_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5517297.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMEMLWWRRR"
     gene            complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO150_003256"
     CDS             complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO150_003256"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5517298.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3425..3919
                     /gene="ppiB"
                     /locus_tag="OO150_003257"
     CDS             3425..3919
                     /gene="ppiB"
                     /locus_tag="OO150_003257"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5517299.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3922..4644
                     /gene="lpxH"
                     /locus_tag="OO150_003258"
     CDS             3922..4644
                     /gene="lpxH"
                     /locus_tag="OO150_003258"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5517300.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4762..5271
                     /gene="purE"
                     /locus_tag="OO150_003259"
     CDS             4762..5271
                     /gene="purE"
                     /locus_tag="OO150_003259"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5517301.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5268..6335
                     /gene="purK"
                     /locus_tag="OO150_003260"
     CDS             5268..6335
                     /gene="purK"
                     /locus_tag="OO150_003260"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5517302.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETVLTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YMGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6472..7363)
                     /locus_tag="OO150_003261"
                     /pseudo
     CDS             complement(6472..7363)
                     /locus_tag="OO150_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(7360..8175)
                     /locus_tag="OO150_003262"
     CDS             complement(7360..8175)
                     /locus_tag="OO150_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR5517303.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8186..9445)
                     /locus_tag="OO150_003263"
     CDS             complement(8186..9445)
                     /locus_tag="OO150_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR5517304.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGGCLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9455..10096)
                     /locus_tag="OO150_003264"
                     /pseudo
     CDS             complement(9455..10096)
                     /locus_tag="OO150_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="multicopy suppressor of dominant negative ftsH
                     mutations; putative acyl-CoA synthetase; internal stop;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2216 a         2862 c         2711 g         2307 t
ORIGIN      
        1 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
       61 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      121 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      181 caaaaagttc aggcgtgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      241 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      301 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      361 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      421 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      481 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      541 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctt
      601 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      661 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      721 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      781 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
      841 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
      901 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgcttca ggcgtgcgtt
      961 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     1021 cgttgagctg tgcgatttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1081 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1141 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1201 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1261 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1321 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1381 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1441 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1501 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1561 gggcttattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1621 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1681 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     1741 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1801 atggaaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     1861 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     1921 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     1981 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2041 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2101 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2161 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2221 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2281 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgtct gcttcaggtt ctcttcgctg
     2341 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2401 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2461 cggtcaacca tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca
     2521 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2581 tggatatcaa agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag
     2641 ccaggacgac ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2701 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     2761 tgcagctggt cgagatcctg acgcgacagc acgccataag tcggatcggt cgggacgtcg
     2821 aacatcacgt cgccgttgtc agccacataa gcgtgacctt tggcgatcag ttgttcagtg
     2881 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     2941 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3001 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3061 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3121 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     3181 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3241 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3301 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3361 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     3421 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgatgataa
     3481 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3541 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3601 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac
     3661 ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt
     3721 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3781 ctactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     3841 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     3901 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     3961 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc tcgcaaggcc
     4021 gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga cgatcccaat
     4081 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4141 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagtggc
     4201 atgacgttat tgccggaaga aaaggtgctc gaactttatg gccgccgggt gttgattatg
     4261 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4321 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     4381 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     4441 gttaaccaaa acgcagtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4501 gggcataccc atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc
     4561 gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4621 gagctgattc attttccgtt ttaaaaaaac cacaactttg ctgattttac agccacgcaa
     4681 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4741 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4801 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     4861 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     4921 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     4981 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5041 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5101 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5161 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5221 gcccagaccg acgaagtgct ggaaaatccg gacccgcgag gtgcggcatg aaacaggttt
     5281 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5341 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5401 tgattaccgc tgagatcgaa cgctggccgg aaaccgtatt aacccgcgag ctggcgcgtc
     5461 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5521 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5581 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5641 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5701 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5761 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     5821 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     5881 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatatgggc gtgatggcga
     5941 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6001 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6061 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6121 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6181 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6241 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6301 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6361 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6421 cgtcaagcgt cgaatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga
     6481 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6541 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6601 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6661 ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6721 ggcattaatc tgctcggcga gcaacgcagt ggcgagatct ttatcaatca ccgcttcact
     6781 ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     6841 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     6901 caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag
     6961 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7021 cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gtatctgcgg
     7081 ctgtgcgctc aagctctgcg ccagcatata gcctcatccc ctggctttcc gccaccagca
     7141 catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca
     7201 cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg
     7261 cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct
     7321 ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa
     7381 ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag
     7441 cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag
     7501 gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt
     7561 ggtaacgagt tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa
     7621 agcgccataa taccaagccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt
     7681 taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc
     7741 ctgaaaacag tgacgaaact ggcgtaattc agcggtcagc ggatcgcttg ccgccatcgc
     7801 cagtggtccg aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg
     7861 aggatgcgaa ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa
     7921 acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc
     7981 gcataacgca tcgaattgcg cgcggcgaag cacccatcct ccggggccga aaccactacc
     8041 ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgccgcca
     8101 cactcccgct aaccgccagg actggcgata attcggtgcg ctactgctgg caagcagagg
     8161 atggatgatc gtcattgcgc gttcctcaac caatccccat gctttcagcc agtgcttcca
     8221 gcgcctgttc aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc tgcccgatcc
     8281 ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt tctaccacgc
     8341 gacgaatatc cagccccagg cacgcgccct gaaaatccca gcctgggatc tgcaactgca
     8401 tattgcgttc aaggtaaatt tccgccatct cttcagatac cgccctggcc gcttccatgc
     8461 cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgca cctccgatac
     8521 caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc
     8581 cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt aaaccagcgt tcacccagcc
     8641 cgcttacccg aatcccgaac atattgccgt tgcgggtcat agccgtaacg atgctgcctg
     8701 cgcggatcat cgcgccagca tccatcgccg ccttgcagta ggccatcgcg aggttgagga
     8761 agaactgatc ggtcacgctg aggaaatcga tcacttccgc aatgtgctgt ttatcatgat
     8821 cgaggcgagc aatttgtggg gccagcgtac gcattaacag tgctgaggaa gcaatattgc
     8881 gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat catcgccgtg agatcgatac
     8941 cgcgctccat gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc
     9001 gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt
     9061 cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca
     9121 tactggcaga agtaatgccg cccattggcc cgacggcatt cacatggtga caaggaatga
     9181 agttcacttc cccctgctcc agcatcgcca gtgcctgcgc ttcatctttc gcccagcctt
     9241 cgaacagaca tccgcccacg cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca
     9301 ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaaaaag
     9361 ccggttgcac atccagccag tgtggacgag cacgacgaat ttgttcgata accgcagcat
     9421 tggcttgcgc cactgatgta aacatgggaa ccccttattg caaacgttct aataaacgag
     9481 ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc ggtttgctgg
     9541 cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg acgttttcca
     9601 gtaatgacgg tgtatgttgc tgtgtggcag gcgagagcgg ataaattaac gctgccgcca
     9661 gcaaggtggc ttccggcagc gaactcacta ccgcgatccc cgcatcttcc agcgtggcga
     9721 tttgctgcga gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcataca
     9781 gtggttgatt atctgaacgc gcggcacagg ctttttgcta ggcgcttacc agcgaggcgg
     9841 caggatcggc agtcgcaccg aagccaatca cgacatcaag tagcaatacg cgcacttgtg
     9901 gtttagcgcc gagatcggca attaactggt tgcgtaaggc tgggtcgatc atcggatggg
     9961 gacgcccgac ggtgtagaaa tcatcgccga ggtcaataat ctggtgacca tcggcgtcga
    10021 gcatcatgcc gtgatgatgg gtatcgtcgg cttccacgcc aaggtgtcca gcaagtaatc
    10081 ccgccgcttc ggcagc
//
LOCUS       DAJTNN010000150        10088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTNN010000150 DAJTNN010000000
VERSION     DAJTNN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.150"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..964)
                     /gene="gapA"
                     /locus_tag="OO150_003265"
     CDS             complement(<1..964)
                     /gene="gapA"
                     /locus_tag="OO150_003265"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5517305.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNK"
     gene            1306..1719
                     /gene="msrB"
                     /locus_tag="OO150_003266"
     CDS             1306..1719
                     /gene="msrB"
                     /locus_tag="OO150_003266"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5517306.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1761..2033
                     /locus_tag="OO150_003267"
     CDS             1761..2033
                     /locus_tag="OO150_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5517307.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2194..2835)
                     /gene="pncA"
                     /locus_tag="OO150_003268"
     CDS             complement(2194..2835)
                     /gene="pncA"
                     /locus_tag="OO150_003268"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5517308.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2846..3862)
                     /gene="ansA"
                     /locus_tag="OO150_003269"
     CDS             complement(2846..3862)
                     /gene="ansA"
                     /locus_tag="OO150_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5517309.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4029..5885)
                     /gene="sppA"
                     /locus_tag="OO150_003270"
     CDS             complement(4029..5885)
                     /gene="sppA"
                     /locus_tag="OO150_003270"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5517310.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDHNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPSAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6046..6597
                     /locus_tag="OO150_003271"
     CDS             6046..6597
                     /locus_tag="OO150_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5517311.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAILEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6714..7757
                     /gene="selD"
                     /locus_tag="OO150_003272"
     CDS             6714..7757
                     /gene="selD"
                     /locus_tag="OO150_003272"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5517312.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQACVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7762..9723
                     /gene="topB"
                     /locus_tag="OO150_003273"
     CDS             7762..9723
                     /gene="topB"
                     /locus_tag="OO150_003273"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5517313.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            9850..>10088
                     /locus_tag="OO150_003274"
     CDS             9850..>10088
                     /locus_tag="OO150_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517314.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYL"
BASE COUNT         2527 a         2476 c         2673 g         2412 t
ORIGIN      
        1 ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg aagttgtcgt
       61 tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg ttgaaatcgg
      121 tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct tcagcagcgg
      181 ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga acggtcaggt
      241 caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt ttgccattca
      301 gttctggcag tactttacct acagctttag cagcaccggt agaggacggg atgatgttct
      361 gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg gttttctgag
      421 tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag ttatcgttga
      481 taactttagc cagcggagcc aggcagttgg tggtgcagga agcgttggaa acgatgtcct
      541 ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg tctttagacg
      601 gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca gtttcgtcag
      661 tcaggaacag accagttgct tcagcgacaa cgtcaacacc aacttcgtcc catttcaggt
      721 tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc agatgaccgt
      781 ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc agcatgtatg
      841 ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca gaacgtttct
      901 gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct actttgatag
      961 tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa ttacaaaaac
     1021 cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc tgtgcctaag
     1081 cattacgcga ctgactcgcc tcactcttcc tttgggcttg aaaccacatg gggtcggcgc
     1141 cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt taggtgcagc
     1201 catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat gttttgttag
     1261 aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc taataaacct
     1321 tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac gcagaatcat
     1381 gggacagagc cgccatttac ggggcgttta ctgcataaca agcgtgacgg cgtatatcac
     1441 tgtttgatct gcgatgcccc gctgtttcat tccgaaacca agtatgattc cggctgtggc
     1501 tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa agacttgtca
     1561 catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct ggggcatgtc
     1621 tttcccgacg ggccgcaacc aacaggcgaa cgttattgtg ttaactctgc ttctttacgc
     1681 ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc agcaacttat
     1741 tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg acgcctgagg
     1801 tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcctgatggc gttgcgttga
     1861 ccgaggaaca aaaagagaat tgcctgcaac tggtgatgct gtggcaagcc cgccataata
     1921 ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag agcaaacagc
     1981 agttaaaaga agattttggt atctcagcaa agcctattgc tatgtttaag tgaatgagtt
     2041 atctagtatt taagcctgtt attaagggag ggcaaatata cttgttcgga cgtcgtgttt
     2101 tagctgcaag taggttaaat gcaacactga caagtattat ctggcctaca atgtcattga
     2161 ggaaatgtag gccagagatg aattcaacta atattacccc tgtgtctctt cccagtctgc
     2221 cagcgtatat agcgttgccc cagctgctga catctccata aacgcgtgtg cactgtcctg
     2281 gggctggata ttcacgccac gacaaccatc ggtaatcacg ttcaccttat aacctaactg
     2341 taacgcgtcc agcacggtaa acttcacgca atagtcagta gccaggccca taacgatcaa
     2401 ttcatcgatt tcatgatcgc gtaaccagtc atcgagagag gttttctgcc gacggccgtt
     2461 atcaaaaaag gcactgtaac tgtcaactaa aggattttcg cctttatgga acaccgctgc
     2521 gatcgctttt tggttcagta acggatgtaa ttgtgcgcct tcactgttct gcacacagtg
     2581 atctggccag aaggtttgtg gcaaaccgtc gagttggcct ggcgtataag gctctacacc
     2641 gtgctgactg gcaaaactgc cgtgattcgc cggatgccag tcctggctgg cgataaccgc
     2701 ttctccgcgc gactggcacc agtcaataag gcggttagca acctccaccg tactgtcacc
     2761 ttccggtacg gcgagcgcgc caccagcaca gaaatcattt tgtaaatcga ccagtaacag
     2821 ggcgcgatgg ggcattgcag tctccttaat catccggcgt cagttcgccg cgcaggtttt
     2881 ggctcatggc cttgcgaatg gtttcagtat ccagttcctg gctcagcagg taatgcagtt
     2941 tggttagcgt ggcttctaca gtcatatctg caccgccaat aacgccggca tgggcgaggg
     3001 cgttaccggt ggcataacca cccatattca ctttaccgga catacattgt gtcaggttga
     3061 ccaccacaat accgcgatcg ctggcttctt gtaattcctg caggaaggct ttgttttgtg
     3121 gcgcattacc cacgccatag gagcgcagaa tcaatgcttt cactggttgg cgcagaaaat
     3181 tgcgcacgac atcagcagaa atccccggat aaatcgtcac tacgccaatt ggttgtggcg
     3241 tgattggatg aacgattaat tccccttcac cgtgcggggc gggtggcgta ttcaaacgac
     3301 gaatatggat accagcttcc agtaacggag gaaggtttgg agaggcaaac gcatcaaaac
     3361 catcggcatg ggctttggta gtgcggttgc cgcgatacaa tcggttattg aaaaagagcg
     3421 ttacttcgtt gatcggataa ttcgccgcaa cgtacaacgc attcagcaga ttaatttgtc
     3481 cgtcagagcg taactcagcc agcgggattt gtgaccctgt cacaatgacc ggtttaccga
     3541 gattctcgag catgaacgac agcgcagagg cggtatacgc catcgtgtcg gtaccgtgta
     3601 gaatgacaaa accatcatag tcgtcatagt gcgctttaat atcttcagca atatgctgcc
     3661 agtcatccgg cgtcatatca gaagaatcca tcaacggcgt atattcatga atggtgaaat
     3721 ctggcatctc cgggcgatgg aattccggca tcagcgccag ttggcgttgt agatgacctg
     3781 acaccggaat ataaccctgc tcggaacgct gcatcccgat ggtcccgccc gtgtaggcga
     3841 cgtaaattga tttcttttgc atgatattga tatgaatgat atcgaaagag caaaagtata
     3901 tacgtgaggc aggaggagcg cacctgatac tatgcggata aggaaatatg gggcgagaca
     3961 gcgggaagag gattcgtcga ggtaaaaaat aatgtggccg acgatctgtc ggccactcag
     4021 tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg cataacggtt ttgtgggtcg
     4081 ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag aggctaccga ggcaagcggt
     4141 gcaggtaaca tggcctggaa cgcatctggc aacattgccc ggacagaacc agacatgttg
     4201 tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt attccagatg ccactgtttc
     4261 acttttgcga gctctgctgc tttggcaacc gcatcatcga aatccccgag actatcgacc
     4321 agcccgttag cttttgcatc ctgaccggtc cagacgtggc cctgggcgat tttatcaatt
     4381 tgctccggcg tcgaatgacg cgcatcagca accagcgtga taaagcgttt atagccattc
     4441 tcaatgctta attgcatcat ctgctgcgct tccggcggca gtgccctggt gatagaaaca
     4501 tccgccagcg gtgaagttga gacaccatca gtatgaacac caatcgaatc cagactattt
     4561 tctacggtgg tgatcacgcc gaagataccg atagaaccgg tcagggtgct ggggttagcc
     4621 acaatgtaat tagctggcgt ggaaatccag taaccaccag atgccgccat gccgcccatc
     4681 gatacaacca caggcttacc cgctgcccgg gctgctgcca gttcagcgcg aatcacttca
     4741 gacgcggtaa cgctgccgcc tgggctatta acacgcagga caatcgcttt cactttcggg
     4801 tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac cgccaacatt cccctgagtt
     4861 tcctcgccat ccataattgc gccattagcg aagacgacac cgatgctgtc accggtatct
     4921 gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc gataattttt atcagtctta
     4981 ctccagccga actctttggt cagagctttt tcgatttcgg cactcgatgc cagtgcatcg
     5041 accagcttgt tttccagtgc atatttcgcg gtatcgccac cggttttggt taaaccctca
     5101 agcaaccctt gcgcgctagg gaatacctgc tgagcaggga tctgtcggtt agcggcaaca
     5161 gtattcagat agttttgcca cagctcacca atccagcggc tgtcagcttc gcgggctgcc
     5221 ggtgacatat catcacgaat aaacggttca acggcagatt tatacgtacc cacgcggaac
     5281 acatgggtgg aaactttcag cttatccagc aacgatttgt agtacagacc gttggtagca
     5341 aaaccgtgca gatcaaccac gccttgcggt gacagccaaa ttttattggc gaaactggcg
     5401 agataatatt gcccctggct gtagttctcg ccaacggcat aaaccggttt cccgctgtca
     5461 cgaaactctt tcagggcttt gccgatgtac tgcatagacg gctggtcgcc gcctgcgaag
     5521 tttttcagat ccatcacgat accggtgata ttgtggtcgt ccttcgcctg gcgaatggtg
     5581 ttgacgatat caaacagtga gttttcctgc agacgatcgg aactggcacc aagcagctgg
     5641 cggcttaatt tactaaaccg ctgagaactg tcgggtttat cgacgatcac accggaaatg
     5701 tccagcagca gtgcgccacg actggccgtt tctttcgaat caccaccact gacctgcatc
     5761 cagatcccca caccaaccag tacgaggaaa ataaagaaca ggttaagtac catttcacgg
     5821 acgaaattca gcagacgcca cgtccattta aaaaatccgg caataaatcg ccaaagggtt
     5881 cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg cgcaatagat gtggttatcg
     5941 taatgaccca accgacactt gtcagcagga attgcggact gtgctgtaac aaaatccact
     6001 ctcgtgttaa ttttgtgaat aaatatcacg acaggagtta atcaaatgga tgcactcgaa
     6061 ctattgatca atcgccgtag cgcctcccgc ctggctgaac ccgcgccaac gggtgaacaa
     6121 ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc ataagtccat gcaaccgtgg
     6181 catttttttg tgattgaagg ggaagggcgc gagcgtttca gcgccatact ggaacagggg
     6241 gcgattgctg ccggtagtga tgacaaagct atcgacaaag cccgtaatgc gccgttccgc
     6301 gcaccgctca tcatcacggt ggtggcgaaa tgcgaagaaa atcataaagt cccgcgatgg
     6361 gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc aaatggcagc cgttgcccag
     6421 ggatttggcg gcatctggcg cagtggcgca ttaactgaaa gtccggtagt gcgtgaagca
     6481 ttcggttgcc gtgagcagga taaaattgtc ggttttctct acctcggcac gccgcagttg
     6541 aaagcatcta cgtcgattaa cgtcccggac ccgacgccgt ttgtaactta tttctgataa
     6601 ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga attcagacac tctcacttat
     6661 cacttcacgg aatgagggtt accatacccg gattgcattg acaggagatg tccatgagcg
     6721 agaactcgat tcgtttgacc caatacagcc acggagctgg ttgcggctgt aaaatttccc
     6781 caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa gtttgttgat ccgaatttgc
     6841 ttgtgggtaa tgagacccgc gacgatgcgg cggtgtacga tctgggcaat ggcaccagcg
     6901 ttatcagtac caccgacttc tttatgccga tcgttgataa tcctttcgat tttggccgca
     6961 ttgcggcgac taacgccatc agcgatatct tcgcgatggg gggcaaaccg attatggcga
     7021 ttgcgatcct cggctggccg attaacaaac tttccccgga aattgcccgc gaagtaaccg
     7081 aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct ggctggcggt cactccatcg
     7141 atgcgccgga gccgattttt ggtctggcgg taacggggat cgtaccgacc gagcgggtga
     7201 agaaaaacag caccgcacaa gccggatgca aactgttcct gacgaaaccg ctggggatcg
     7261 gcgttcttac cacggctgag aaaaaatcac tgctgaaacc agaacatcag ggactagcga
     7321 cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt tgccaacatc gaaggcgtaa
     7381 aagcgatgac cgacgttacg ggctttggtc tgctgggcca tttgagcgaa atgtgtcagg
     7441 gcgcaggtgt gcaggcgtgc gttgactatg acgcgatccc aaaattgccg ggggttgaag
     7501 agtacattaa gttgggcgca gtgcctggcg gcaccgaacg taactttgcc agctacggtc
     7561 atctgatggg tgaaatgccg cgtgaagtgc gcgatttgct gtgcgatccg caaacttctg
     7621 gcggtttgct gctggcggtc atgccggaag cagaaaatga ggtcaaagct acagccgccg
     7681 agtttggcat tgaactgacg gcaattggcg aactggtgcc agcgcgcggc ggtcgtgcca
     7741 tggttgagat tcgttaattc aatgcggttg tttattgccg aaaaaccgag tctggcgcgc
     7801 gccattgctg atgtcctgcc caaaccgcac cggaaaggcg atggctttat cgagtgcggt
     7861 aatggtcagg tggtgacctg gtgtatcggt cacctgcttg agcaggcgca gccagacgcc
     7921 tacgacagcc gctatgcgcg ctggaatctt gcggatttgc cgattgtccc ggaaaagtgg
     7981 caattacagc cccgaccctc cgtgaccaaa caacttaacg tcatcaaacg cttcctgcat
     8041 gaagccagcg aaatcgttca cgccggggac ccggatcgtg aagggcaact gctggtggat
     8101 gaagtgctgg actatctgca actggcaccg gaaaagcgcc agcaggtgca gcgttgcttg
     8161 ataaacgacc tgaacccgca ggcggttgag cgggcgatcg accgtcttcg gtccaacagt
     8221 gagtttgtgc cattgtgcgt ttctgcgctg gcgcgagcgc gtgccgactg gctgtacggc
     8281 atcaatatga cccgtgcgta taccattctc ggtcgcaatg ccggttatca gggcgtactt
     8341 tccgtgggac gcgtgcagac acccgtgctc gggctggtgg tgcgccgcga tgaagagatt
     8401 gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac atatcgtgac acctgccgat
     8461 gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg aaccgtacca ggatgaagaa
     8521 gggcgcttgt tacatcgtcc actggcggag catgtggtta accgcattag tggtcaacca
     8581 gcaattgtca ccagctataa cgataaacgg gaatcagaat ccgcgccgct gcctttttcg
     8641 ctttcggcgt tgcagattga agcggcaaaa cgctttggtc tgagcgcgca gaacgtgctt
     8701 gatatctgcc agaagctgta cgaaacgcac aagctaatca cttatccgcg ttccgattgt
     8761 cgctatttgc cagaagaaca ttttgccgga cgccacgcgg tgatgaatgc catcagcgtt
     8821 catgcaccag atctgttgcc gcaaccagtg gtagatccag atatacgcaa ccgctgttgg
     8881 gatgacaaaa aggtcgatgc acaccacgcc atcatcccga ccgcacggag ttctgcgatc
     8941 aacctgacgg agaacgaagc gaaggtctat aacctgattg cccgtcagta cttgatgcag
     9001 ttctgcccgg atgcggtgtt ccgcaagtgt gttatcgaac tggacattgc caaaggcaaa
     9061 tttgtcgcta aagcgcgttt tcttgctgaa gcaggctggc gcacgctgtt aggcagcaaa
     9121 gagcgcgatg aagaaaacga cggtacgcca ttgcctgtgg tggcgaaagg cgatgagttg
     9181 ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc cgccgcgcca ttttaccgat
     9241 gcaacactgc tttcggcgat gaccgggatc gcgcgctttg tgcaggacaa agatctgaaa
     9301 aagatccttc gtgcgaccga tggtctgggg acagaagcaa cccgtgccgg gattattgaa
     9361 ctgctgttca agcgtggttt tctgaccaaa aaagggcgct atatccactc taccgacgcc
     9421 ggaaaagcgt tattccattc gctgccagaa atggcgacgc gaccggacat gaccgcgcac
     9481 tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc gctatcagga ctttatgcag
     9541 ccgctggtgg ggacgctata tcagcttatt gatcaagcca aacgtacgcc ggtgcggcag
     9601 tttcgcggca ttgtggctcc gggcagtggt ggcagtgctg ataagaaaaa ggctgcaccg
     9661 cgtaaacgta gtgcgaaaaa aagtccgcca gcagatgaag tcggaagcgg ggcgatagcg
     9721 taagcgagtg aatctttcgt gctattcgag tcatattctg aaatatccag cgaatcaaga
     9781 aaattcgttg gatatttttt tgcatggata aaattatcgc ctctaaagta tgtaataaca
     9841 gggaatgtga tgaaaaaagt tttattacaa aaccatcctg ggagcgagaa gtattctttt
     9901 aatggctggg aaatatttaa tagtaatttt gaacggatga ttaaagaaaa taaggccatg
     9961 ctgctttgta agtgggggtt ttatttaaca tgtgttgtcg ctgtaatgtt tgtgttcgca
    10021 gcgataacat ccaacggttt gaatgaaaga ggcctgatta ccgcgggatg ctcttttctt
    10081 tatctatt
//
LOCUS       DAJTNN010000151        10048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTNN010000151 DAJTNN010000000
VERSION     DAJTNN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.151"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..206
                     /locus_tag="OO150_003275"
     CDS             <1..206
                     /locus_tag="OO150_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517315.1"
                     /translation="STLKSPSYTKSVSWQHYRHICCYRRKLTDAELEACYRYDKQFWE
                     HYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(323..1666)
                     /gene="gdhA"
                     /locus_tag="OO150_003276"
     CDS             complement(323..1666)
                     /gene="gdhA"
                     /locus_tag="OO150_003276"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5517316.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVGAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1902..2174
                     /locus_tag="OO150_003277"
     CDS             1902..2174
                     /locus_tag="OO150_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5517317.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2140..2547)
                     /gene="nudG"
                     /locus_tag="OO150_003278"
     CDS             complement(2140..2547)
                     /gene="nudG"
                     /locus_tag="OO150_003278"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5517318.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            2634..3245
                     /locus_tag="OO150_003279"
     CDS             2634..3245
                     /locus_tag="OO150_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5517319.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFISTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3254..4561)
                     /gene="ynjE"
                     /locus_tag="OO150_003280"
     CDS             complement(3254..4561)
                     /gene="ynjE"
                     /locus_tag="OO150_003280"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR5517320.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4628..5281)
                     /locus_tag="OO150_003281"
     CDS             complement(4628..5281)
                     /locus_tag="OO150_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5517321.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5281..6816)
                     /locus_tag="OO150_003282"
     CDS             complement(5281..6816)
                     /locus_tag="OO150_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5517322.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPM
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCTLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALAAKWVGYVRQ
                     GLR"
     gene            complement(6789..7955)
                     /locus_tag="OO150_003283"
     CDS             complement(6789..7955)
                     /locus_tag="OO150_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5517323.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WDDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(7965..8513)
                     /locus_tag="OO150_003284"
     CDS             complement(7965..8513)
                     /locus_tag="OO150_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5517324.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8513..9220)
                     /locus_tag="OO150_003285"
     CDS             complement(8513..9220)
                     /locus_tag="OO150_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5517325.1"
                     /translation="MMKMQSRKIWYYCITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9235..9912)
                     /gene="ydjY"
                     /locus_tag="OO150_003286"
                     /pseudo
     CDS             complement(9235..9912)
                     /gene="ydjY"
                     /locus_tag="OO150_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
     gene            complement(9917..>10048)
                     /locus_tag="OO150_003287"
     CDS             complement(9917..>10048)
                     /locus_tag="OO150_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5517326.1"
                     /translation="LQLCLAGLALFILVQLAKLYARHKHVDLSASRRSPLTHPKNEG"
BASE COUNT         2400 a         2740 c         2591 g         2317 t
ORIGIN      
        1 caagcacctt aaaatcacca tcatacacta aatcagtaag ttggcagcat taccggcata
       61 tctgctgcta tcgcagaaag ttaacggatg ctgaacttga agcgtgttat cgttacgaca
      121 agcagttttg ggagcactac agtaagaaat gccgttggcc cataagaaat gttccctggg
      181 gagcatcatc cgttaaatac tcataaacgc ctgaaatttt gccgggggcg ctgcgcttgc
      241 ccggcctaca ttagagcgta actgcaatga attgtgccca tttgtaggcc tgataagcgt
      301 agcgccatca ggcatttaca acttaaatca caccctgcgc cagcatcgca tcggcaacct
      361 tcacaaaacc ggcaatgttc gcgccctgca cgtagttggt ttgctcacct tcaccaccat
      421 gctcaacaca ggcatggtgg atatccagca tgatgtgatg caaacgtgcg tcaactttct
      481 cggctttcca gcccaggcgc gcagcgtttt gcgccatttc aaggcctgac gttgctacac
      541 cgccagcatt agccgcttta cccggtgcaa acagtacgcc tgcctgctgg aacagttcag
      601 tcgcttcgat ggtggtcggc atatttgccc cttcggcgac ggctttaacg ccattggcga
      661 taagctgatg cgcggcgcca acatccagtt cgttctgggt ggcgcaaggc agggcgatat
      721 caaccggtac agaccacggc tgttggcctt cgagatagac cagaccaaat tctttggcgt
      781 aatctgccac tcgaccatcg cggctggctt tgatttcgat aagacgtgcc agtttctctt
      841 tcgtgaatcc gctttcatca actacagtgc cgctggagtc tgacgcagtg atcacacgag
      901 caccaaattc catcgctttt tcgatagcgt actgggcgac gttgccggag ccagaaacgg
      961 aaacgcgcat cccttcaaaa cccataccgt ggcgttttaa cattgcttct gtgaaataaa
     1021 ccagaccgta gccggtagct tccgggcgaa taagactacc gccaaatgaa aggcccttac
     1081 cggtgaagac gcaggcggta ttgttggaga gctttttcat catccccgcc ataaagccga
     1141 cttcacgacc accaacgccg atatcacctg ccggaacgtc agtatccgcg cccagatgac
     1201 gataaagctc ggtcatcagc gcctggcaga aacgcatcac ttcgctttcg cttttacctt
     1261 tcgggtcaaa atcgctaccg cctttaccac cgcccatcgg cagggtagtc agggcatttt
     1321 tgaaggtttg ttcgaagccg aggaatttga gaatggagag gttaactgac ggatggaagc
     1381 gcataccgcc tttgtacggg ccgatggctg agctgaactg cacacgccat gcacggttga
     1441 cctgtacctg gttgcgatca tcaacccata ccacgcgaaa ctggatcacg cgctccggtt
     1501 caaccagacg ctccagtaat gacatctggc gatattttgg attttgttca agaaaaggcc
     1561 agagtgtggt cattacttca cgaacggctt gcgcgaactc ggtttgattc gggtcgcgct
     1621 tttggacatg gttgaggaat gactccagag aatatgtctg atccatagat ataaaaccct
     1681 tatatattaa tacgattgtg cttatgttgt ttatgtcatg tgcttttgca gttttcgact
     1741 ataccatcaa gaatgtgacg gtaagcaaga aaaaaatgac ccaggaaagc aaattaataa
     1801 cgagagtaat ctcataacca aatgcgataa ctcagacgct aaagttttgc cacccggcta
     1861 ccgttatcag ttgcagagac aataacgtta aggagttccc tgtgagtcga gcattgttcg
     1921 ccgtagtatt agcatttcca ttaattgcac tggctaaccc gcattatcgt ccggacgtgg
     1981 aggtaaacgt accgccagaa gtgttcagtt caggcgggca gtcagcgcag ccctgtactc
     2041 agtgctgcgt ttatcaggat caaaattatt ctgaaggggc ggtaattaaa gccgaaggaa
     2101 tactgctgca atgccagcgg gatgacaaaa cccttagcac taatccgctg ctctggcggc
     2161 gcgtaaagcc ataaacgcct ctaatagtgg aatgtcagta ggggccagcg gatattgcag
     2221 cgcctcttca ggtgagcacc agaccagcgc ctgatgttca tgtgcctgta acgtcccgtg
     2281 gaagtcgggt acgtgccagg catgaagatg gataatccgg cccgaaactt ctcgctgatg
     2341 gctggcaaca tactcaccca cagttgcttc gatgcccaat tcttcgttta actcacgcac
     2401 cagtgcctgc tgctggcttt catccagctc gactttacca ccggcaaact cccataatcc
     2461 cgcctgatcg ctctgggcgg ggcgttgcgc gagtaaaatt ttgccatcac gttcaatgat
     2521 ggcggcaaca acttcaatca ttttcataaa ttacacacgg gcagataagc gaaaaatgcc
     2581 ataatatccg cactgttgct caatcagaaa ggctttatat ttaaggagtg cctgtgctag
     2641 accgccatct tcatccccgg attaaaccgt tgttgcatca gtgcgtgcgg gttcttgata
     2701 aaccgggcat tacgccagat ggtttaacat tagtgggatt tgccatcggc gtgctggctc
     2761 tgccgtttct ggcgctgggc tggtatctgg cggcgctagt cgtcattttg ttgaacaggc
     2821 tgcttgatgg tctggacggt gcgctggcgc ggcgcagaga gcttaccgac gcgggcggct
     2881 ttcttgatat ttctctcgat tttctctttt acgcgctggt gccgtttggc tttattcttg
     2941 ctgcaccgga gcaaaatgcg ctggcaggcg gctggttgtt gtttgcgttt atcagcacgg
     3001 gcagcagttt tctcgccttt gctgcactgg cagcaaaaca tcagattgat aaccccggtt
     3061 atgcacataa gtcgttttac tatctggggg gattaaccga aggcacagaa acgatcttac
     3121 tgtttgtgct gggatgttta ttccctgcat ggtttgcgtg gatcttcggt gcgttgtgct
     3181 ggatgacgac ctttacgcgg gtgtggagtg ggtatctgac gctgaagtcg ctccagcgtc
     3241 agtaattgca atgttatttg ctactgtccg ggccgcgttc accggttgct accggatttt
     3301 ttggatcgct gctccattcg taccagccgc cgtcatacac ggaaacattc ttccaaccca
     3361 tggcgcgtgc gtacataaag gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa
     3421 cttgctgctc tggtttgata ttccacgctt tccacatagc ggtaatatca tcggcgctgc
     3481 gcatggtgcc atccgggtta tgaaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc
     3541 cccaacgtgc tccggctatt tcaccttttg gtttaatata gctgtaaccg ctggtcgtac
     3601 cgataaattc tggccacgaa cgaatgctca ccagcgatgc atcctggcga tgcagcagtc
     3661 cacgggcctg ttccatatcg agcatcaact gcggttgtgc cgggatcttc acgccgaaat
     3721 ccggttccgc tttcactttc ggtggcgttc ctcgctcaac aggcagtccc gcgtcggacc
     3781 aggtttgcca gccgccatcc agcagacgca catctttcac gcctgcgtag agcataattt
     3841 gcgccacacg cgctccagcg tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc
     3901 gaatgccgtg ttttgccagc atcgctttta gttgttcatc agaaactttg ttccacagcg
     3961 gttcgctttc cacttcgttg gtatcgatgt agtcagcacc gggaatatgg ctgataaggt
     4021 aaagcttagg agcgccccag gccgcttcaa tgactttcca gtcaccggca ggtttcgccg
     4081 taacctcttt accttgttgc aggtcgtgca gccattgcgg ataaaccagc tgctcaaagt
     4141 gcggcagttt ttgtagacgg gaaggttcgc ttagcgcgtc actcagaatg gagatatgcg
     4201 taaaacctgc tttttgcagt cgcgttttga cggcgtcgac atctttgtca ttaccgtaca
     4261 gcgccaccgg ggcatcggct ttcaggttat gttgcttgat ccacgcgttg agctgttcgg
     4321 tgctcatttt gtcgagccag ctggcagaga ggtttaaggc ggcaggttca tgaccagaag
     4381 ggccatttaa ggtttgtggc cagccgttat aaaacgcgct ggggcgagta tctatcgctt
     4441 tgccattttg ttgttgaagc tggtcaagtg taagaggctt cgccagttca gcggcccacg
     4501 aagaagcgca agccagccct aaagccattg ccagcgcggt catttgagaa acacgtttca
     4561 tcgaaaatcc cgacattgta gttttgagtg cgccattctg aactgacgca ttgagaaaaa
     4621 ctttgcgtta tcgcagtttg ttgtaatttt ctgaccactg cgccatatcc agaacagaac
     4681 tatcagcagg aacatcctgg agatcgtgcg ttacctgaac gacggggatc gccagttcgc
     4741 gaacttcgct gaacacccac tggcgaaaat tatcgcgcag agccacatca agacggctga
     4801 atggctcatc caggagtaac gcttttggct gagcgagaag ggcgcgcagc agggccaccc
     4861 gcgctcgctg accgcctgat aatgtcgcag ggtcctggtg ataagtttca gccagtcccg
     4921 ctcgatcaag cgcgtctttc acggcattgc gcctggcagt ccccttgagc gtcgacggta
     4981 gcgccagcag taaattttgc ccgacgctga actggtcaaa taacagtgca tcctggaaaa
     5041 gaatgccaat ctgacgctgt gcggtgggta gcatgtcaat ccgttgctca ttgagccata
     5101 gctcacctgt acaagaaaac tgtccagcca gtgcaccaat catccatgaa aacagagtgg
     5161 attttccaca gccagacggc cccattaacg tgacgatgtc acctttatca accgtaaagt
     5221 taacgtttgt cagcaagcgg ctttctggta aacgtagcga aacatttttc acgcagagca
     5281 ttagcggagt ccttgtctga cataacctac ccattttgcg gctaacgcgg tcagggcaaa
     5341 aataataagc ggtaacaaca gttgccataa agcctgggcg gcgagaatac cgttgctgcc
     5401 gccgctgctt aatgccaccg cctcagtggt gagcgtcgga aaacgccccg cgcccagcca
     5461 cagcgtcggc atatactgcg caatactgac tgaaaatccc accgcgaagg caatcagcac
     5521 agggcgcaac atgagtgtgc atttcacgta aaagaagatt ttggcccgcg accagcccag
     5581 tgtttgcgca atcaaaatta accgtgaatc aatgcgctgc caggcgggtt gcaggataaa
     5641 cagcatccac ggcattaccc acagcagatg cccccagacc accgccgtcc agcttccatc
     5701 caggttcagc catagcgcca gcgtgtactg gcccgccacc agtggcagag cgggcaatag
     5761 aatgggcaac catagccaca actggcgacg ctgtggcccc cattccagcc acagcagtaa
     5821 caggagcaaa gcgatgaatg ttgctaccag ccccattgtc agactgttga tgagtgcttc
     5881 actattgatc gtcgactgat ccgcgaggat cgccagcaga accacacaca gcacaccggt
     5941 taagggtaaa aaactcgcca gcgtagtgcc tggtaataaa ggtgtggcag gcttgcgaat
     6001 gccatctacg cggggaatag tgcgccgcca gctgcgccat agcagatagc tcagcaaaac
     6061 gtaggcggcg agtaacagca tcagcagcag actggcgagt gcgcctttcg tttgttgatc
     6121 cgcgtcgccc tgagttaacc actgccagct aattactgcc agcatcggtg gattacctgg
     6181 cccaagaata atcgccacat ccacgaccga aagcgaccag gcaacaatcg ccagcatcgc
     6241 cattgccagc gaaggcgcga cggagggcaa cagcagccag ttcaggcatt gccagcggct
     6301 gtagcccagc gaatccagca caatgacctg ctgcaacagc cgtttttcgc tcaatactgc
     6361 cgctaagatc cacagcagaa atgcgctttc tttcactgca agggtgaggc ccagaccgat
     6421 gccaaatcgg tccattggcg gagtgaaata cgggaaatag tcatatagca gccctccgtc
     6481 agcaaagagc agcagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg
     6541 cagacgggcg cacatacgct gccatttcgg cccaggccat agcgccacaa tcaccagcag
     6601 ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc
     6661 atgcggtaac tgtggatcgg caaataacgc ctgccagtgt gtcaacgaca acgcaggcga
     6721 tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccacca tcgcccacag
     6781 caaaaagatt aatgcgtacc gtaacggcgt aaccattctt gttccagtgc attcacccat
     6841 cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattcttga ttgcaatatt
     6901 tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgtc ccagacaacg
     6961 ggatctgctt tacgcagttg cgcatcgggt gagagcagaa aattggcaac taccttcgcc
     7021 gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat gcacgttgcc aatcatcccc
     7081 tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc cgctggcgat tttttgctgc
     7141 gcatgcgcgg ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag cagagcatcc
     7201 atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca
     7261 tcaagatatt gccacaaggg cgcagtgaca cgggcgaaag tcgcatcgtc cggcgcttct
     7321 tttaatgcgg cgggatctgg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg
     7381 gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat tggctttagc aaactccagt
     7441 aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt
     7501 tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca ctgaaaaatc ttcccgcacc
     7561 ggcagctgtg tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat
     7621 aaattggcct cttttaaggt gcggaagttt tcgccgttca cccagagcag atccaccgag
     7681 ccgcccgttt tacgaccggc agcagcttcg gtctgaatgc gcttcaccgc gtctgcggca
     7741 tccgcgagac gaacaatctt caggtttata gcgtaatggg ttttcatctc gccgctaacc
     7801 cagtcgagat agcggttaat tgcggtatcg ccgccccagg cgttaaacca gacggtttgc
     7861 cctttggcct catttttaat ttcctgccag tcactggcat gtgttgccaa agaaaacagc
     7921 gataacaatc ccagcaacca cccacaatgg cgcatcttct ctccttaaga acgtttttcc
     7981 ggaatacggc acagcccctg agcgggaatg tccatggcag aattaaaacg agccgacagg
     8041 ttttgcaggc cgatcaatgc cgtcagttcg gtgagcgcct gagcgtcaaa atgcgcagcc
     8101 agtcgggtac gcagggcatc atcgactgtt ggcggtgtga cgcttgcggc ttcggcgtac
     8161 tccagcgcca gccgttcttc atcgctaaag agcgggcttt gccgccagtc agccactgcc
     8221 agcagtttat cggtgctgcc ggtacgctcg gcgactttca tactggtgat atccacacaa
     8281 aactcacaca ggcacatttg cgcaatgcgc gcgctgacaa gcgatcgcac taccggatcg
     8341 agcggtgagc gtttgcgctc cagccaccca acaaacattg acaccaaata aaagataaac
     8401 gggatccgcc cccaccagcg aatcgggctt agcacctcgc cgtaatggcg acgatgcagc
     8461 cacgcctgcg gacgtaaaat gaaaggaatt ttgctaagcg gcggtaaacc catcagtcat
     8521 tcctcttctg gcgctcaagc catattttct tcgccataaa aatcaccacg gtcagggcaa
     8581 acagaataaa cagtccggtg acaaaccaga aagtaccgcc tgttaacatg ctgcccgccc
     8641 aggaataaac aatagtcgcc ggtaattgac caagcccggt ggcgataaaa aacgagcgaa
     8701 aacgtattga agtcagaccg gcagcatagc tgattggatc gaaagggaca aaaggcaata
     8761 accgacagac caggatggtg tgtttgccgt agcgagtgaa aaagccgtcc atactgtcaa
     8821 gcacggtttt gccggttaat ttttccacca cttcgcggcc catcactctg gcgataaaaa
     8881 agcacagcgc cgcgccggcc atcgaactgg tccacgacag caagccgccc cagaacgcgc
     8941 caaacagcga cgcattggca aaggtgatca aaaacgcagg tagcggtgca gcaatggcct
     9001 gtaaaatcat caacaagaac gagacaactg ccgccagtgc gccgtaagac tgaataaagc
     9061 gttctatacc ctgttggtcc acggcggcaa acgccgcaac gctgcgattg ataaactcat
     9121 ggacgccggg aagcagcgcc catgcgagca gcatagcgaa caacaggatg atgagggtta
     9181 tacagtagta ccagatttta cgcgattgca tcttcatcat cctttatcct ggctttattc
     9241 ggtgatttta aaggtaaccg ttgccagcgt gttatccgcc gggagaactg aggcattgcc
     9301 tttgaatttc acttcgccgc gtttttccac cgcaccataa gtgtatgttg cattgctgac
     9361 gatgccgacc gggcagctat ccagacacac cagtcaaccc gttttcttct cttctgctgc
     9421 cgttaaatta ccgccaaagc gcatgtccag tttcttgcca ttactgtcaa caatcacttc
     9481 atcgaaggaa tacgcttttg ccgccccttg ccagttgacc gaaatatcca gtttgctgcc
     9541 tgtgacatga gtcgtttctt tattatccat cgtcatgttt tcgcccggcg taccacctgc
     9601 ctctttcagg gcttcataaa atgctttcgg ggtcgcataa gccataaaca gcgatttatg
     9661 tccgttggag ccatctttaa acacaatacc gtgacgagtg tcgtcggtga ggaaacgacc
     9721 attaatttgc accagcatgg ttaccgtatg gttattggca tcgacgcgca gcggttggct
     9781 gttcgaaagt ccgtcgtatt ccacttttgc cgccgaattc tctttttgat cgcagccgct
     9841 aagcccggca acagccagta agcagagtgc agccagtcct tttttccatg acactgaata
     9901 atgttgcgac atcgttctat ccttcatttt ttgggtgagt aagcgggctg cggcgcgaag
     9961 cagacagatc cacatgtttg tgtcgggcgt agagttttgc gagctggacg agaataaaca
    10021 gcgccaggcc cgccagacag agttgtaa
//
LOCUS       DAJTNN010000152         9958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTNN010000152 DAJTNN010000000
VERSION     DAJTNN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.152"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            260..457
                     /locus_tag="OO150_003288"
     CDS             260..457
                     /locus_tag="OO150_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517327.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(407..1600)
                     /locus_tag="OO150_003289"
     CDS             complement(407..1600)
                     /locus_tag="OO150_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5517328.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1736..3460
                     /gene="iucA"
                     /locus_tag="OO150_003290"
     CDS             1736..3460
                     /gene="iucA"
                     /locus_tag="OO150_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5517329.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3461..4408
                     /gene="iucB"
                     /locus_tag="OO150_003291"
     CDS             3461..4408
                     /gene="iucB"
                     /locus_tag="OO150_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5517330.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4408..6150
                     /gene="iucC"
                     /locus_tag="OO150_003292"
     CDS             4408..6150
                     /gene="iucC"
                     /locus_tag="OO150_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5517331.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6147..7484
                     /gene="iucD"
                     /locus_tag="OO150_003293"
     CDS             6147..7484
                     /gene="iucD"
                     /locus_tag="OO150_003293"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5517332.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVITLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7490..9685
                     /gene="iutA"
                     /locus_tag="OO150_003294"
     CDS             7490..9685
                     /gene="iutA"
                     /locus_tag="OO150_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5517333.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWIIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2396 a         2565 c         2645 g         2352 t
ORIGIN      
        1 tcataaaaaa catgtcagtg aaaaagaaac ctggcgttgg gttgaaggat ataataaagc
       61 aacagaactg gctcgccaat gccctgatac ccatgtattg tcaatttctg atcgtgaagg
      121 cgatttttat gacctgtttg aacgcgcaga gcagactccc ggaataaaag ctgactggct
      181 tgtcagaatg aaatttaata acagagcaac gctgaatatt aatggtaaac gggatcatag
      241 gttgttgcac gaaagaatca tggagattac accgcagcaa ctggttgaat tcactatacc
      301 tgatgggcgg gggcagcaag ccagaacact gcctgacatg ctttcatcag acgcaggtcc
      361 tctctcatat cctctgattt actccagtaa ggtgatgatc atatctttat ttgatagtac
      421 ggtaatgcca gataaataca gacacaatgg taaataatat agttgcaaat aatgctgcca
      481 taatcagcgc gattgcgaca cctgttttcg ataccagcgg caccagcaaa gcgcacagtc
      541 catagccaag tgtgtgactg gtagcgagcc agcccgcgcc tgcgccatca gccagcttat
      601 cattcagtag aagttgataa gcgggtgttg ccagagctgc tccaaatgac agcactgcac
      661 agcccgggta aaacaaccat agctgttcgg agagcatgat agccagacca ccactcatca
      721 acactccggc actcaggagc agggccaccg gagtcaggcg ctgcggacgg actaccccga
      781 actgcgcgat aagcgcagct actgcggaca gacccaacag ccaggccacc tgttggctaa
      841 tggcggtggt atccgtgacg aactggcgag taagggcagg cgaaagtcca agctgcatca
      901 tgctcaccgc cgcagccagt aatattgcgc aaagcagata aggcagacaa tcccgcttca
      961 gactgacgct cttgcactcc ggtgtgggct gtggtggtgt gccgggcaac cgcagcagca
     1021 tcagcagcgc cagcactggt gctgccatca gtagccccag tggcgccaga ggatgaatgg
     1081 ccagcattgc tgccgcgcac agcggcccga atagcctgcc gcaactcagg ccggagctga
     1141 tggttgccag agcggccatg cgattccctt ctccagctct ctgcaatgcc cagacctgac
     1201 aggctggcac cattgccgac acggtcagcc cgtaggcgat ccgggcgatg attaatattc
     1261 ccagtcccac cgctgttgtt atggcgctgg tagccatcag cacgcttccc agccctagca
     1321 gggtaaagct tatgccatat cctcccagag cccacaacac cacaggttta ctgccagtac
     1381 gggaaatttg ctttccccac cacggggacg atggcagaaa cagcattgag ccaagcatca
     1441 gcagggcagc ccatacagac agactcagat ttgtctgtat gaccagaaca gggagcacaa
     1501 ccagcagacc gttctgtccg ataccgagaa gcccggcact gaacgcaagt ggccagcagg
     1561 acagtgtttt ttggggcgta tcttcgatcc ctggtgacat tataatgctt caactccatg
     1621 tattaattgt gtttatttgt aaaattaatt tatctgacaa taacatttat tgttgataat
     1681 gagaatcatt atcgacataa ttgttattat tttactgtgt gggagctgtt tgactatgac
     1741 cctgccctct gaaaaaccag ccacagatgt ggctgcgcag tgcttcctga atgcactgat
     1801 tcgtgaaacc acagactgga aactgacaga atacccgcca gacgaattgc tcatcccgct
     1861 ggatgagcag aaatcgctcc atttcagagt ggcttatttc tccccaaccc agcatcaccg
     1921 ctttgcattt cctgcacgtc tggtcacggc atcaggcagt tatcctgtcg actttaccac
     1981 tctctcccgg cttattattg ataagctacg ccatcaactg tttctgcccg ttcccctctg
     2041 cgaaactttc caccagcgcg tgctggaaag ccatgtccat acgcaacagg caattgatgc
     2101 ccgtcatgac tgggccgctc tgcgtgaaaa agcgttgaat tttggcgagg ctgagcaggc
     2161 gctgctgaca ggacacgctt tccaccctgc gcctaagtct catgaaccgt ttaaccggcg
     2221 ggaggctgaa cgctacctgc ctgatatggc accccacttc ccactgcggt ggttttcggt
     2281 ggataaaacg caaatcgccg gtgaaagttt gcatctcaac cttcaacagc ggctgacgcg
     2341 atttgccgca gagaatgcgc ctcagttact caacgaatta agtgacaacc aatggctgtt
     2401 cccgttgcac ccgtggcagg gggaatatct tttgcagcag gggtggtgcc aggcacttgt
     2461 tgctaaaggg ctgattaaag acttaggtga ggccggcacg tcgtggctgc cgaccacctc
     2521 ttcccgttcc ctctactgtg ccaccagccg cgatatgatc aagttctccc tgagcgtacg
     2581 gctgaccaac tccatccgta ctctgtccgt gaaagaagtg aagcgtggga tgcgcctggc
     2641 acgcctggct caaaccgacg gctggcagat gctacaggtc cgcttcccga ctttccgggt
     2701 aatgcaggag gatggctggg ccgggctgct cgatcttaac ggcaacatca tgcaggaaag
     2761 tctgtttgcc ctgcgtgaaa atctgctggt ggatcagccg aaaagccaga ctaacgtact
     2821 ggtctccctg actcaggccg cacctgatgg cggtgattcg ctgctggttt cggcggtaaa
     2881 acgcctgagc gatcgcctcg gtatcactgt gcaacaggcc gcccatgcat gggtcgatgc
     2941 atactgtcag caagtgctaa agccgctgtt tacggctgaa gcggattacg gcctggtgct
     3001 gctggcgcat cagcaaaata ttcttgtcca gatgcttggg gatctgccgg tcggatttat
     3061 ttaccgtgac tgtcagggca gcgcttttat gcctcacgcg acagactggc tcgattctat
     3121 tggcgaggcg caggcggaaa atattttcac ccatgagcag ttgctgcgct attttcctta
     3181 ttacctgctg gttaactcca cttttgctgt gaccgctgcg ctgggggctg ccgggctgga
     3241 cagcgaatcg aatctgatgg ctcgtgtacg agcatcgctg gctgaagtgc gtgatcaggt
     3301 gactcataaa acctgtctca actacgtgct ggaaagtccg tactggaacg taaaaggtaa
     3361 ctttttctgt tatctgaacg atcataacga gaacaccatt gttgaccctt cggtgatcta
     3421 tttcgatttc gctaacccgc tgcaggctca ggaggtctga atgtctgagg caaacattat
     3481 tcacagcaga tatggactgc gctgtgaaaa actcgacaag cccctgaatc ttggctgggg
     3541 actggacaat agcgcggtgt tgcactgtcc cggggagctg ccgacagggt ggctgtgcga
     3601 tgcgctggat cagatattta tcgccgcacc acaactgtca gcagtggctc tcccctgggc
     3661 cgaatggcgt gaggagccac aggcgctgac gctttttggg caggtaaaaa gcgacattat
     3721 ccatcgtact gccttctggc agttaccgtt atggttgagt tcaccggcaa accgggcctc
     3781 cggtgaaatg gtttttgatg cagagcgtga gatttatttc ccgcagcgcc caccccgtcc
     3841 acagggtgag gtttaccgcc gttacgatcc acgtattcgc aggatgctga gtttccgcat
     3901 tgccgatccc gtttctgatg cagaacgttt cacccgctgg atgaatgatc cgcgcgttga
     3961 gtatttctgg gagcaaagtg gctcactgga ggtacagacc gcctatctgg agcgccagct
     4021 caccggtaaa catgcattcc cgctgattgg gtgcttcgac gatcggccat tcagctattt
     4081 cgaaatctac tgggcggcgg aagaccgcat tggtcgtcac tattcgtggc agccctttga
     4141 ccgtggcctg catctgctgg ttggtgaaca gcaatggcgc ggggcccact atgtgcaaag
     4201 ctggctgcgc gggctgacac attacctgct gctggatgag ccccgtacgc agcgcacagt
     4261 actggagcca cgcaccgata accagcgcct gttccgccat cttgagcctg cgggataccg
     4321 gacaattaaa gagttcgact tcccacataa gcgctcgcgc atggtgatgg cggatcgtca
     4381 tcacttcttc acggaggtcg gtctgtaatg aatcacaagg actgggattt tgttaaccgt
     4441 cggctggtgg caaaaatgtt gtctgagatg gagtatgagc aggttttcca cgcggaatct
     4501 cagggcgatg accactactg cattaacctg ccgggagcac aatggcgctt catcgctgaa
     4561 cgtggtatct ggggctggct gtggattgat gcccaaacgt tgcgctgcac ggacgaacca
     4621 gtactggcgc agacgctgct gatgcagcta aagccggtgc tgtcaatgag tgatgccact
     4681 gttgcagagc atatgcagga tttgtatgcc acgctactgg gcgacctgca actgctgaag
     4741 gcccgtcgcg gactgagtgc cagtgacctg attaatcttg atgccgatcg tctgcaatgc
     4801 ctgctgagcg gtcatcctaa attcgttttt aataaaggtc gccgtggctg gggcaaagag
     4861 gcgctggaac gatatgcgcc agagtatacc aacaccttca gactgcactg gctggcggta
     4921 aaacgtgaac atatgatctg gcgctgcgat aacgatctgg atattcagca gttgttgact
     4981 gccgcaatgg atccgcagga gtttacacgc ttcagtcagg tctggcagga aaacgggctg
     5041 gatcacaact ggctaccgct gccggtacat ccgtggcagt ggcagcaaaa aatcgccaca
     5101 gacttcatcg ctgattttgt cgaaggcagg atggtgtctc tcggcgagtt tggcgaccag
     5161 tggctcgccc agcagtcgct gcgtaccctg accaacgcca gccgccgggg agggctggat
     5221 atcaagctgc cgctgaccat ctacaacacc tcatgctacc gggggatccc tggcagatat
     5281 atcgctgccg gaccactggc ttcacgctgg ctgcaacagg tttttgcgac cgacgccacc
     5341 ctggtgcaaa gcggcgcagt gatccttggt gaaccggcgg caggctatgt gtcccatgaa
     5401 ggctatgccg cgcttgcccg ggctccctat cgctaccagg aaatgcttgg tgttatctgg
     5461 cgggagaatc cgtgccgctg gctgaaaccg gatgaaagtc ctgttctgat ggcaacactg
     5521 atggagtgcg acgaaaacaa tcagccgctg gcaggcgcat atatcgaccg ctccggactg
     5581 gacgctgaaa cctggcttac gcaattgttc cgggtggttg tggttccgct gtatcacctg
     5641 ctttgccgct acggtgtcgc gcttattgca cacggacaaa atataacgct cgccatgaaa
     5701 gagggggtac cacagcgtgt tctgctgaaa gacttccagg gcgatatgcg gctggtgaaa
     5761 gaagcgtttc ccgaaatgga ctctttgcct caggaggtgc gtgatgccac atcccgtctg
     5821 agtgcggact acttaatcca tgatttgcag acgggtcact tcgtgacagt actgcgtttt
     5881 atttcgccac tgatggttcg tcttggcgta cctgaaaggc gattttatca actgctggca
     5941 gcagtgttga gtgattacat gaacaaacat ccacaaatgg cagagcgttt tgcgcttttc
     6001 tcactcttca ggccacaaat cattcgcgtg gtgctgaacc cggtaaaact gacctggccg
     6061 gacctggatg gcggcagtcg catgctgccg aattaccttg agaatctgca aaatccgctg
     6121 tggctggtaa ctcaggaata tgaatcatga aaaaaagtgt cgattttatt ggtgtcggga
     6181 cagggccatt taatctcagc attgctgcat tgtcacatca gatcgaagaa ctgaactgtc
     6241 tcttctttga cgaacatcct catttttcct ggcatccggg tatgctggta ccggattgtc
     6301 atatgcagac cgtctttctg aaagatctgg tcagtgcagt tgcacctaca aatccctaca
     6361 gctttgttaa ctatctggtg aagcacaaaa agttctatcg cttccttaca agcaggctac
     6421 gtacagtatc ccgtgaagag ttttctgact atctccgctg ggctgctgaa gatatgaata
     6481 acctgtattt cagtcatacc gttgaaaata ttgatttcga caaaaaaagt cgattgtttc
     6541 tggttcagac cagtcgggga gaatattttg cccgcaatat ctgccttggt acaggaaaac
     6601 aaccttattt accaccctgt gtgaagcatg tgacacaatc ctgtttccat gccagtgaaa
     6661 tgaatcttcg tcggccggac ctgagtggaa aacggataac cgtggttggt ggaggacaga
     6721 gtggtgcaga cctgttcctt aatgcattac gcggggaatg gggagaagcg gcggaaataa
     6781 actgggtctc acggcgtaat aattttaacg cactggatga ggctgctttt gctgatgagt
     6841 attttacacc tgaatatatt tcaggcttct ccggactgaa ggaagatatt cgccatcagt
     6901 tactggatga gcagaaaatg acatcggatg gcatcactgc cgattcttta ctgaccattt
     6961 atcgtgagtt gtaccaccgt tttgaagttc tgagaaaacc aagaaatatc cgtctgctac
     7021 ccagtcgctc ggtaataact ctggaaagta gtggtccggg ctggaggtta ttgatggaac
     7081 atcatctgga tcagggcaga gagagcctgg aaagtgatgt ggtgattttc gccacaggtt
     7141 accgttctgc attgccacaa atacttccct cactgatgcc cttgatcacc atgcacgata
     7201 agaacacctt taaagtgcgt gatgacttca ctctggaatg gagtggcccg aaagaaaata
     7261 acatctttgc ggtcaacgcc agcatgcaaa ctcatggcat cgccgaaccc cagctcagcc
     7321 tgatggcctg gcgatctgca cgtattctta atcgcgtact ggggcgtgat ttattcgatc
     7381 tcagtatgcc gcccgcactg attcagtggc gcagcggcag ccggaaaaaa ccgcagccgg
     7441 aggctgcttc tttaactcac tatacagcaa atattcagga ataacgatga tgcgcaaaaa
     7501 gtatatgccc cgggctcttg gtccgctgct tcttgtcgtg ctgtcaccag ctgtcgccca
     7561 gcaaaacgat gataatgaga tcatagtgtc tgccagccgc agcaatcgaa ctgtagcgga
     7621 gatggcgcaa accacttgga ttatcgaaaa tgccgaactg gagcagcaga ttcagggcgg
     7681 taaagagctg aaagacgcac tggctcagtt aatccccggc cttgatgtca gcagccagag
     7741 tcgaaccaac tacggtatga acatgcgtgg ccgcccgctg gttgtcctga ttgacggtgt
     7801 gcgcctcaac tcttcacgtt ccgacagccg acaactggac tctgtcgatc cttttaatat
     7861 cgaccatatt gaagtgatct ccggcgcgac ggccctgtac ggtggcggga gtaccggagg
     7921 gttgatcaac atcgtgacca aaaaaggtca gccggaaacc atgatggagt ttgaggctgg
     7981 cacaaaaagt ggctttaaca gcagtaaaga tcacgatgag cgcattgccg gtgctgtctc
     8041 cggcggaaat gaccatatct ccggacgtct ttccgtggca tatcagaaat ttggcggctg
     8101 gtttgacggt aacggcgatg ccaccctgct tgataacacc cagaccggcc tgcagcactc
     8161 caatcggctg gacatcatgg gaaccggtac gctgaacatc gatgaatccc ggcagcttca
     8221 actgataacg cagtactata aaagtcaggg agacgacaat tacgggctta atctcgggaa
     8281 aggcttttcc gccatcagcg ggagcagcac accatacgtc agtaaggggc tgaattctga
     8341 ccgcattccc ggcactgagc ggcatttgat cagcctgcag tactctgaca gtgatttcct
     8401 gggacaggaa ctggtcggtc aggtttacta ccgcgatgag tcgttgcggt actacccgtt
     8461 cccgacggta aatgcgaata aacaggcgac ggctttctcc tcgtcacagc aggataccga
     8521 ccagtacggc atgaaactga ctctgaacag ccaactgatg gacggctggc aaatcacctg
     8581 ggggctggat gctgagcatg agcgctttac ctccaaccag atgttcttcg atctggctca
     8641 ggcaagtgct tccggagggc tgaacaacca taagatttac accaccgggc gctatccgtc
     8701 atatgacatc accaatctgg cggccttcct gcaatccagc tatgacatta atgatatttt
     8761 taccgttagc ggtggcgtac gctatcagta tactgagaac agggtagatg atttcatcga
     8821 ctacacgcag caacagaaga ttgctgccgg gaaggcgata tctgccgacg ccattcctgg
     8881 tggttcggta gattacgata actttctgtt caatgctggt ctgctgatgc acatcaccga
     8941 acgtcagcag gcatggttca atttttccca gggggtggca ttgccggatc cggggaaata
     9001 ttatggtcgc ggcatctatg gtgcagcagt gaacggccat ctttccctga caaagagcgt
     9061 gaacgtcagc gacagtaagc tggaaggcgt gaaagtcgat tcttatgaac tgggctggcg
     9121 ctttaccggt gacaacctgc ggactcaaat cgcggcatat tactcgcttt ccaataagag
     9181 cgtggaaagg aataaagatc tgaccatcag tgtgaaggac gacaggcgcc gtatttacgg
     9241 cgtggaaggt gcggtggact acctgatccc ggatactgac tggagtaccg gtgtgaactt
     9301 caatgtgctg aaaaccgagt cgaaagtgaa cggtcaatgg caaaaatatg acgtgaagga
     9361 atcaagtcca tcgaaagcga cagcttacat taactgggcg ccggaaccgt ggagtctgcg
     9421 tgtacagagc accacttctt tcgacgtaag cgatgcagag ggtaacgata ttaatggtta
     9481 cactaccgtc gattttatca gtagttggca gcttccggtg ggaacactca gcttcagcgt
     9541 tgagaacctc ttcgaccgtg actataccac tgtctgggga cagcgtgcac ctctgtacta
     9601 cagcccgggt tacggccctg cttcactgta cgactacaaa ggccggggcc gaacctttgg
     9661 tctgaactac tcagtgctgt tctgacgggg atgcttttgc aacaagggta tgctgtaaac
     9721 aacatggtcc ttggtggcca tgttgtttac agccagtata atatacagcc ttaacaagat
     9781 attccgtctc cggaatggac tgttcaccac tgaataacat tatttctgtt ttatggatct
     9841 ttgactgaaa tttatcgcat atcctcattt cattgtccgt ctgaaaacta accttacaag
     9901 taagcgtcgt ctcagcaccg tctggcagat cctgaaattc ctgagaggat gatctgcg
//
LOCUS       DAJTNN010000153         9896 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTNN010000153 DAJTNN010000000
VERSION     DAJTNN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.153"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO150_003295"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO150_003295"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5517334.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO150_003296"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO150_003296"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5517335.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3389)
                     /locus_tag="OO150_003297"
                     /pseudo
     CDS             complement(2698..3389)
                     /locus_tag="OO150_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3519..5279
                     /locus_tag="OO150_003298"
     CDS             3519..5279
                     /locus_tag="OO150_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5517336.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5685..6542
                     /gene="focA"
                     /locus_tag="OO150_003299"
     CDS             5685..6542
                     /gene="focA"
                     /locus_tag="OO150_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5517337.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6597..8879
                     /gene="pflB"
                     /locus_tag="OO150_003300"
     CDS             6597..8879
                     /gene="pflB"
                     /locus_tag="OO150_003300"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5517338.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9071..9811
                     /gene="pflA"
                     /locus_tag="OO150_003301"
     CDS             9071..9811
                     /gene="pflA"
                     /locus_tag="OO150_003301"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5517339.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2536 a         2488 c         2355 g         2517 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtttc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgtccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgaag atggaataat ccagtatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgagg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgttttccc ccgctttcat atgaatgatt tcactacaac gaatacaggc atagtccgcc
     2821 gactccggca ccactttgta acgctgcgta caatcactgg gtaaaataag taaacccggt
     2881 ttcaccttgt catcttcaaa gaaatagaca ctgatttgcc cgttggtttc gagaatcgcc
     2941 agacgtacct gccccagctg ctccacgcca cgcaatcgta gctccataaa gaactcaaat
     3001 tccgtcatgt tggagttatt gagtttcgac caggccagct cgccatcttc aataataaca
     3061 accggcttac cttccagtag atcttccagt ttttcactgt gcgccatcaa ccacattacc
     3121 aggcggtata acaacgccag agtgataaag acgataagta ccggcaccat cggcacatca
     3181 tcataaaacg ccacatctcc cgccgctgaa cccagcgtga gaatgattaa cacttcaaac
     3241 agcgacatct gtcgcacacc gcgtcttccg gtcattttga ggaacaaaaa aaccagtaca
     3301 aaggtataaa gactacgtag tgcaacttcg ccaaggaaat caaaaggcac tttatcaaat
     3361 gccatacggt ggagatcgaa cgccttcatt tttatgctct tatcgtggtt tattcgctaa
     3421 aagcatagtc aacagttcat cggcatggcg gcggtgatag cgcctttgag ataaaacccg
     3481 tattattgcg cgctttccgt acgactaaag tgattttcat gacgcaaaca tttatccccg
     3541 gcaaagatgc cgctctggaa gattccatcg ctcgcttcca gcaaaaactt tcagacctcg
     3601 gctttcagat tgaagaggcc tcctggctga atcctgtgcc taacgtctgg tctgtacata
     3661 ttcgcgacaa agagtgcgca ctgtgtttta ccaacggtaa aggcgcaacc aaaaaagcgg
     3721 cgctggcttc tgcacttggt gaatatttcg agcgtctctc aaccaactac ttttttgctg
     3781 atttctggct gggcgaaacc atcgccaacg gtccgttcgt gcattatccc aacgaaaaat
     3841 ggttcccact gaccgaaaat gacgatgtgc cagaaggact actcgatgac cgtctgcgcg
     3901 cgttttatga tccggagaat gaactgaccg gcagcatgct gattgaccta caatccggta
     3961 acgaagatcg tggtatttgc ggcctgccgt ttacgcgtca gtccgacaat cagaccgttt
     4021 atattccgat gaatatcatt ggtaacctgt acgtctccaa cggtatgtcc gcaggtaata
     4081 cccgcaacga agcacgcgtt cagggattgt ctgaagtttt cgaacgctac gtgaaaaacc
     4141 gcattattgc tgaaagcatc agcctgccag agatcccggc agacgtgctg gcgcgttacc
     4201 cagcggtggt tgaagcgatc gaaacactgg aagcagaagg tttcccgatc ttcgcatatg
     4261 atggttccct tggcggccag tatccggtga tttgcgtggt actgttcaat cctgctaacg
     4321 gtacctgctt tgcctctttc ggtgcgcatc ctgattttgg cgtagcactg gaacgtaccg
     4381 tgaccgagct gctgcaaggt cgtggcctga aagatttgga tgtgtttact ccgccaacct
     4441 tcgatgatga agaagtcgct gaacatacca acctcgaaac gcactttatc gattccagcg
     4501 gtttaatctc ctgggacctg ttcaagcagg atgccgatta tccgtttgtg gactggaatt
     4561 tctccggcac cacggaagaa gagtttgcta cgctgatggc tatcttcaac aaagaagata
     4621 aagaagttta tattgccgat tacgagcatc tgggcgttta tgcttgccgt attatcgtgc
     4681 ctggcatgtc cgatatttat ccggctgaag atctgtggct cgcgaataac agtatgggca
     4741 gccatttacg tgaaacgatt ctttcgctac caggcagcga gtgggaaaaa gaagattacc
     4801 tgaacctcat cgagcaactg gatgaagaag gttttgatga ctttacccgc gtgcgtgagc
     4861 tgttgggtct ggcgaccggg tcggataacg gttggtacac cctgcgtatc ggtgaattaa
     4921 aagccatgct ggcgctggct ggtggcgatc tggaacaggc tctggtctgg accgaatgga
     4981 cgatggagtt taactcatca gtatttagtc cggaacgcgc caactattat cgctgcctgc
     5041 aaacgttgtt attactggca caggaagaag atcgccagcc gctgcaatat ctgaatgcgt
     5101 ttgttcgcat gtacggcgca gatgccgtgg aagccgccag tgcggcaatg agcggcgaag
     5161 cggcgtttta cggcctgcaa ccggtagata gcgatctgca cgcgtttgct gcacatcagt
     5221 cgttgttgaa ggcctacgaa aagctgcagc gcgccaaagc agcattctgg gcaaaataaa
     5281 atcaaatagc ctacgcaatg taggcttaat gattagtctg agttatatta cggggcgttt
     5341 ttttaatgcc ccgctttaca tatatttgca ttaataaaat aattgtaatt ataaggttaa
     5401 atatcggtaa tttgtattta ataaatacga tcgatattgt tactttattc gcctaatgct
     5461 cccttttaat taactatttt agcggaggat gcggaaaaaa ttcaactcat ttgttaattt
     5521 ttaaaattta tttttatttg gataatcaaa tatttactcc gtatttgcat aaaaaccatg
     5581 cgagttacgg acctataagc caggcgagat atgatctata tcaatttctc atctataatg
     5641 ctttgttagt atctcgtcgc cgacttaata aagagagagt tagtgtgaaa gctgacaacc
     5701 cttttgatct tttacttcct gctgcaatgg ccaaagtggc cgaagaggcg ggtgtctata
     5761 aagcaacgaa acatccgctt aagactttct atctggcgat taccgccggt gttttcatct
     5821 caatcgcatt cgtcttctat atcacagcaa ccactggcac aggcacaatg cccttcggca
     5881 tggcaaaact ggttggcggc atttgcttct ctctggggct gattctttgt gttgtctgcg
     5941 gagctgatct ctttacttcc accgtgttga ttgttgttgc taaggcgagt gggcgcatca
     6001 cctggggtca gttggcgaaa aactggctaa atgtctattt tggcaacctg gtcggcgcac
     6061 tgctgtttgt acttttaatg tggctttccg gcgagtatat gaccgcaaat ggtcaatggg
     6121 gactaaacgt cctacaaacc gccgaccaca aagtgcacca tacttttatt gaggccgtct
     6181 gtcttggtat cctggcaaac ctgatggtat gtctggcagt atggatgagt tattctggcc
     6241 gcagcctgat ggacaaagcg ttcattatgg tgctgccggt cgcgatgttt gttgccagcg
     6301 gttttgagca cagtatcgca aacatgttta tgatcccgat gggtattgta atccgcgact
     6361 tcgcatctcc ggaattctgg accgctgtcg gttctgcacc ggaaaatttt tctcacctga
     6421 ccgtgatgaa cttcatcact gataacctga tcccggttac gatcggtaat attatcggcg
     6481 gtggtttgtt ggttgggttg acatactggg tcatttacct gcgtgaaaac gatcaccatt
     6541 aatggttgtc gaagtacgca gtaaataaaa aatccactta agaaggtagg tgttacatgt
     6601 ccgagcttaa tgaaaagtta gccacagcct gggaaggttt taccaaaggt gactggcaga
     6661 atgaagtaaa cgtccgtgac ttcattcaga aaaactacac tccgtacgag ggtgacgagt
     6721 ccttcctagc tggcgctact gaagcgacca ccaccctgtg ggacaaagta atggaaggcg
     6781 ttaaactgga aaaccgcact cacgcgccag ttgactttga caccgctgtt gcttccacca
     6841 tcacctctca cgacgctggc tacatcaaca agcagcttga gaaaatcgtt ggtctgcaga
     6901 ctgaagctcc gctgaaacgt gctcttatcc cgttcggtgg tatcaaaatg atcgaaggtt
     6961 cctgcaaagc gtacaaccgc gaactggatc cgatgatcaa aaaaatcttc actgaatacc
     7021 gtaaaactca caaccagggc gtgttcgacg tttacactcc ggacatcctg cgttgccgta
     7081 aatctggtgt tctgaccggt ctgccagatg catatggccg tggccgtatc atcggtgact
     7141 accgtcgcgt tgcgctgtac ggtatcgact acctgatgaa agacaaactg gcacagttca
     7201 cttctctgca ggctgatctg gaaaacggcg taaacctgga acagactatc cgtctgcgcg
     7261 aagaaatcgc tgaacagcac cgcgctctgg gtcagatgaa agaaatggct gcgaaatacg
     7321 gctacgacat ctctggtccg gctaccaacg ctcaggaagc aatccagtgg acttacttcg
     7381 gctacctggc tgctgttaag tctcagaacg gtgctgcaat gtccttcggt cgtacctcca
     7441 ccttcctgga tgtgtacatc gaacgtgacc tgaaagctgg caagatcacc gaacaagaag
     7501 cgcaggaaat ggttgaccac ctggtcatga aactgcgtat ggttcgcttc ctgcgtactc
     7561 cggaatacga tgaactgttc tctggcgacc cgatctgggc aaccgaatct atcggtggta
     7621 tgggcctcga cggtcgtacc ctggttacca aaaacagctt ccgtttcctg aacaccctgt
     7681 acaccatggg tccgtctccg gaaccgaaca tgaccattct gtggtctgaa aaactgccgc
     7741 tgaacttcaa gaaattcgcc gctaaagtgt ccatcgacac ctcttctctg cagtatgaga
     7801 acgatgacct gatgcgtccg gacttcaaca acgatgacta tgctatcgct tgctgcgtaa
     7861 gcccgatgat cgttggtaaa caaatgcagt tcttcggtgc gcgtgcaaac ctggcgaaaa
     7921 ccatgctgta cgcaatcaac ggcggcgttg acgaaaaact gaaaatgcag gttggtccga
     7981 agtctgaacc gatcaaaggc gatctcctga actacgatga agtgatggag cgcatggatc
     8041 acttcatgga ctggctggct aaacagtaca tcactgcact gaacatcatc cactacatgc
     8101 acgacaagta cagctacgaa gcctctctga tggcgctgca cgaccgtgac gttatccgca
     8161 ccatggcgtg tggtatcgct ggtctgtccg ttgctgctga ctccctgtct gcaatcaaat
     8221 atgcgaaagt taaaccgatt cgtgacgaag acggtctggc tatcgacttc gaaatcgaag
     8281 gcgaataccc gcagtttggt aacaatgatc cgcgtgtaga tgacctggct gttgacctgg
     8341 tagaacgttt catgaagaaa attcagaaac tgcacaccta ccgtgacgct atcccgactc
     8401 agtctgttct gaccatcact tctaacgttg tgtatggtaa gaaaactggt aacaccccag
     8461 acggtcgtcg tgctggcgcg ccgttcggac cgggtgctaa cccgatgcac ggtcgtgacc
     8521 agaaaggtgc tgtagcgtct ctgacttccg ttgctaaact gccgtttgct tacgctaaag
     8581 atggtatctc ctacaccttc tctatcgttc cgaacgcact gggtaaagac gacgaagttc
     8641 gtaagaccaa cctggctggt ctgatggatg gttacttcca ccacgaagca tccatcgaag
     8701 gtggtcagca cctgaacgtt aacgtgatga accgtgaaat gctgctcgac gcgatggaaa
     8761 acccggaaaa atatccgcag ctgaccatcc gtgtatctgg ctacgcagta cgtttcaact
     8821 cgctgactaa agaacagcag caggacgtta ttactcgtac cttcactcaa tctatgtaat
     8881 tagatttgac tgaaatcgta cagtaaaaag cgtacaataa aggctccacg aaagtggggc
     8941 cttttttagc acgagagcct tttttgtcag ctatctatac tttaaggtga ctgccaaaac
     9001 agactcgacg tagccttcga gctgcgcacc aacacggcct cagatgggcc acatctggag
     9061 aaataccgca atgtcagtta ttggtcgcat tcactccttt gaatcctgtg gaaccgtaga
     9121 cggcccgggt attcgcttta tcaccttttt ccagggctgc ctgatgcgct gcctgtattg
     9181 tcataaccgc gacacctggg acacgcatgg cggtaaagaa gttaccgttg aagatttgat
     9241 gaaggaagtg gtgacctatc gccactttat gaacgcttcc ggcggcggcg ttaccgcatc
     9301 cggcggtgaa gcaatcctgc aagctgagtt tgttcgtgac tggttccgcg cctgcaaaaa
     9361 agaaggcatt catacctgtc tggacaccaa cggttttgtt cgtcgttacg atccggtgat
     9421 tgatgaactg ctggaagtaa ccgacctggt aatgctcgat ctcaaacaga tgaacgacga
     9481 gatccaccaa aatctggttg gagtttccaa ccaccgcacg ctggagttcg ccaaatatct
     9541 ggcgaacaaa aatgtgaagg tgtggatccg ctacgttgtt gtcccaggct ggtctgacga
     9601 tgacgattca gcgcatcgcc tcggtgaatt tacccgtgat atgggcaacg ttgagaaaat
     9661 cgaactcctc ccctaccacg agctgggcaa acacaaatgg gtggcaatgg gtgaagagta
     9721 caaactcgac ggtgttaaac cacctaagaa agagaccatg gaacgcgtga aaggcattct
     9781 tgagcagtac ggtcataagg taatgttcta atttccccgt aaagcggcaa ctttattgag
     9841 ttgccgcttt tttattccgc gcttcattct gctacagtgt ttttaacaca ttttat
//
LOCUS       DAJTNN010000154         9785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTNN010000154 DAJTNN010000000
VERSION     DAJTNN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.154"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            123..743
                     /gene="fklB"
                     /locus_tag="OO150_003302"
     CDS             123..743
                     /gene="fklB"
                     /locus_tag="OO150_003302"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5517340.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1052..2464
                     /gene="cycA"
                     /locus_tag="OO150_003303"
                     /gene_synonym="dagA"
     CDS             1052..2464
                     /gene="cycA"
                     /locus_tag="OO150_003303"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5517341.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFLG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(2509..3171)
                     /gene="ytfE"
                     /locus_tag="OO150_003304"
     CDS             complement(2509..3171)
                     /gene="ytfE"
                     /locus_tag="OO150_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5517342.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYNMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(3279..4244)
                     /locus_tag="OO150_003305"
     CDS             complement(3279..4244)
                     /locus_tag="OO150_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5517343.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIVVRAKPEKPLTESVSES"
     gene            complement(4352..5212)
                     /gene="qorB"
                     /locus_tag="OO150_003306"
     CDS             complement(4352..5212)
                     /gene="qorB"
                     /locus_tag="OO150_003306"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5517344.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLTDSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            5301..5681
                     /locus_tag="OO150_003307"
     CDS             5301..5681
                     /locus_tag="OO150_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5517345.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(5810..7753)
                     /gene="cpdB"
                     /locus_tag="OO150_003308"
     CDS             complement(5810..7753)
                     /gene="cpdB"
                     /locus_tag="OO150_003308"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5517346.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDRHIAFASPDE
                     NRSVLTAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            7943..8683
                     /gene="cysQ"
                     /locus_tag="OO150_003309"
     CDS             7943..8683
                     /gene="cysQ"
                     /locus_tag="OO150_003309"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5517347.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            8895..>9785
                     /locus_tag="OO150_003310"
     CDS             8895..>9785
                     /locus_tag="OO150_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517348.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKE"
BASE COUNT         2388 a         2358 c         2503 g         2536 t
ORIGIN      
        1 cgggatattg ctattgtttc tttttccccg ggatttgtca tcattcctgt gacaaagttt
       61 tacccaaaga agtgtggctg atatgctgcc cgctactttt ataccctaag aaaggaaata
      121 cgatgaccac cccaactttt gacactatcg aagcgcaagc aagctacggc attggtttgc
      181 aggtagggca acaactgagt gaatctggcc tggaagggct gctgccagaa gcactggttg
      241 caggtattgc cgatgcgctg gaaggcaaac atccggctgt tccggttgat gtggtgcatc
      301 gcgcgctgcg tgaaatccac gaacgcgccg atgccgttcg tcgtcagcgt ttccaggcga
      361 tggctgctga aggtgtgaaa tacctggaag aaaacgccaa aaaagaaggt gtgaatagca
      421 ccgaatctgg cctgcaattc cgcgtgatca accagggtga aggcgcaatt ccggcacgta
      481 ccgaccgcgt tcgtgttcat tacaccggta aactgatcga cggcaccgtg tttgacagct
      541 ccgttgctcg tggtgaaccc gctgaattcc cggttaacgg cgtgatccct ggctggattg
      601 aagcactgac tctgatgccg gtaggttcta aatgggaact gactatcccg caggaactgg
      661 catatggcga gcgcggcgca ggtgcatcca tccctccgtt cagcaccctg gtgtttgaag
      721 tcgaactgct ggaaatcctc taagcagcgc attctgttcc cctcgaacca gaggggagca
      781 ggcattcagc aataaaccct tcagtttgcc aaacggcgct attttgtgtt gcaaagaccc
      841 cgtaagcgtg tatttttgtg agatgtttcg cgttatcacc gtgatatgac actcacttta
      901 aacataaaat taacattaga tctaaatctt agtattcatc ccgcgtattg ttacctaata
      961 tcgatgagtc ccgatacaga ttcgtcgtat catagactga ctaaaggccg tagagcctga
     1021 acaacacaga caggtacagg aagaaaaaaa catggtagat caggtaaaag tcgttgccga
     1081 tgatcaggct ccggctgaac agtcgctacg gcgcaatctc acaaaccgac atattcagct
     1141 tattgccatt ggcggtgcca ttggtacggg gttgtttctg gggtctggca aaacgattag
     1201 ccttgccggg ccgtcgatca ttttcgttta tatgatcatt ggttttatgc tctttttcgt
     1261 gatgcgggca atgggggaat tgctgctttc gaatctggaa tacaaatctt ttagtgactt
     1321 cgcttccgat ttactcgggc cgtgggcagg atatttcact ggctggactt actggttctg
     1381 ctgggttgta accggtatgg cagacgtggt ggcgatcacg gcttatgctc agttctggtt
     1441 ccccgatctc tccgactggg tcgcctcgct ggcggtgata gtgctgctgc tgacgctcaa
     1501 ccttgccacc gtgaaaatgt tcggtgagat ggagttctgg tttgcgatga tcaaaatcgt
     1561 cgccatcgtg tcgctgattg tcgtcggcct ggtcatggtg gcgatgcact ttcagtcacc
     1621 gactggtgtg gaagcgtcat tcgcgcattt gtggaatgac ggcggctggt tcccgaaagg
     1681 tttaagtggc ttctttgccg gattccagat agcggttttc gctttcgtgg ggattgagct
     1741 ggtaggtaca acagctgcgg aaaccaaaga tccggagaaa tcactgccac gcgcgattaa
     1801 ctccattccg atccgtatca ttatgttcta cgtcttcgcg ctgattgtga ttatgtccgt
     1861 gacaccgtgg agttcggtag tcccggagaa aagcccgttc gttgagctgt ttgtgttggt
     1921 aggtttgcct gcggctgcca gcgtgatcaa ctttgtggtg ctgacctctg cggcgtcttc
     1981 cgctaacagc ggcgtcttct ctaccagccg tatgctgttt ggtctggcgc aggaaggtgt
     2041 ggcaccgaaa gcgttcgcta aactttctaa gcgcgcagta ccagcgaaag ggctgacctt
     2101 ctcctgcatc tgcctgctcg gtggcgtggt gatgctgtat gtgaatccta gcgtgattgg
     2161 tgcgttcacg atgattacaa ccgtttccgc gattctgttt atgttcgtct ggacgattat
     2221 cctttgctcg tacctggtgt accgcaaaca gcgtcctcat ctgcatgaga agtcgatcta
     2281 caagatgccg ctcggcaagc tgatgtgctg ggtatgtatg gcgttctttg tgttcgtggt
     2341 cgtgttgctg acactggaag atgacactcg ccaggcgctg ctggttaccc cgctgtggtt
     2401 tatcgcactg gggctgggct ggttgtttat tggtaagaag cgggctgctg aactgcggaa
     2461 ataaccgcat tatcatgctg gatggcgcaa tgccatccag cttttagatc actcacccgc
     2521 cagcgcgcgt gggaacagta cattgttttc cagactgatg tgatccatca ggtcatcaat
     2581 cagttcatta atgccgttat acatcgcttt ccaggtggtg caggcttctg gcggcggtgt
     2641 gacgttattg gtagtgtgtt tgatcacttc cagcagttcg cccgcttcat cgtgctcgct
     2701 ttccattacg ctgattggcc ccattgcctg gctgcccatg ccttgtttga tcatcgggaa
     2761 gaggatctgc tcttctttca tcatgtggct ggaaagctct tcatgcagca tggtcaggta
     2821 ttttgtcagc ccttttggca cgctcggttt gtcggcgtga acgcgctcga ctttagtcgc
     2881 ttgcaggatc agctccggca gttgctcgcg gtgacgatcg tggtagcgca cgatgatatg
     2941 gtcaatgatt tctgccagcg gggcgctacg ccagtctttc tcaatcggtt gttcagcaag
     3001 ctttgccagt tcagcttcaa tgacctcaac atccagttct ttacgtgccg ccgcgcgcgc
     3061 cagcgtctgc ttaccgccac agcagtaatc catattatat ttacgaaaca gagctgaagc
     3121 gcgaggaata gagagcgcca gttcacctaa aggttggtcg cgataagcca tagctgatac
     3181 ctcattctta ataagataag atgtatttta aatacatctt taaggcaaaa gctataaccc
     3241 ttacgtagca gaggcttatt taactcacag ccagtatgtc aactttctga gacagattca
     3301 gttaaaggct tttccggttt tgctctgact acaatgacca cgccaaccac taacagcgcg
     3361 ataccgctca gcgtcatcag cggcggcatt tgctggcgta tcaaaaaggt gtacagcaaa
     3421 cctgccagtg tttcgaaaac gatcagcggc ccgagaatca ctgtcggtaa tcgctggctg
     3481 gcgacgttcc agcagagtgc gccaacccat gagcaaagca cggctatcgc aaccatcaga
     3541 ctaataaaca ccagcggacg ggggccaaaa ggtagggaga agtccggcgt ttgcgtattc
     3601 agccagtaac aggcgacgag atagccgatg agagaaaccg gcagtgtgac cagcgcctgc
     3661 gccgtcgccc acatcatcgg atgtttgtcg ggattctccc gcagccagcg ggcgttgcgc
     3721 agggcatacc atgcccagca gaccacggaa actaacgcta gcacgatgcc tgaggtataa
     3781 cgtgcccagt caaaatcggg gagtccgtgg tttaactcag caatattcac acacgccagg
     3841 ccgatgccaa tacaaaccag tgccggggcg agttttcccc acgcgagttt gccgtcgcgc
     3901 tggctataaa gcagattggc aaaaacggga atcaccaccg gcagggtgcc gataatcatc
     3961 gtggaaacag gcgcaccagt acgttgaatg gcactggcaa ggcagaagta atagatgagg
     4021 ttgcccatca tagtgagcat caaggcggta agccagtcgc gacgcgccaa ctgacgcaga
     4081 cgcacgcgtc ccagccaggc aatgggcagc gcaattaacc ctaacgccag ataacgcccc
     4141 atcgactgca acatcgccgg gtattccggt acgatcaacg gcccgacaaa aataagcccc
     4201 cacatcaacc ctgctaacag ggcgtacagc acgccgctaa tcattactgg catccattgg
     4261 tctgtcagaa gaaagttcag gaagcagaca tgattgctcc cggcatcccg tcattattag
     4321 agaggatgcg ggaggatgcc actttaatta actagttatt aacattaaaa agatggctta
     4381 cgctttcggc taacgttgtc gttgggcggc caatcaattt gctaagcgtt ttgctgttat
     4441 caaacagacc acctttcgat gcgccaacgt cagaatccgt cagcatatcc gccagtccgt
     4501 cgggcagtcc gacgcttttc agtgccgcgg cgaaatcggc ttcgctcaga ttttgatagg
     4561 taacctgttt gccgctctgt ttggtcagct ctgccgctaa ctgtgtcaac gtccaggcac
     4621 tatcgcccgc cagttcgtaa accttgcctt cgtgaccggc ttcgctaatc acgcgtgccg
     4681 cagctgccgc ataatctgcc cgcgttgctg aggcgatttt gccatcgccc gccgcaccga
     4741 taaatacgcc gtgttccagt gctgccgggg cgctggcgag gtagttttcg ctgtaccagc
     4801 cgttgcgcag cagggtgtaa acgatgccag aatcagccaa cattttctcc gtctcaatgt
     4861 gctcatcggc gaggccgagc ggggaggtat ctgcatgtag caggctggta taagcgataa
     4921 atttcacgcc agccgccttt gcggcattaa taacattacg atgctgcggg gcacgttgac
     4981 ccacttcgct ggaagagatc agcagtagtt tttccactcc ctgaagtgca gatgtcagtg
     5041 cggcttcatc gccgtagtca gcctgacgca cggtaatgcc ttgtgctgcc agagcctggg
     5101 cttttgccgg attacgaacg atagccacta tttggctggc aggaaccgtt ttcatcaagg
     5161 attcaataat atagtgacca agttggccag tggcaccagt aatagcgatc atgggaagtc
     5221 tccatcgttt tgtcttgtgt ttaaatacgc taacacctaa acttactttt agtaagtacg
     5281 tacaaaaagg taagtatgaa atgagtcagg ttagcctgtc gcaacaactg aaagagggta
     5341 atctctttgc ggaacagtgc ccgtcgcgcg aggtgttgaa acacgtcacc agccgttggg
     5401 gggtgttgat tctggtggcg ctacgcgaag gtactcatcg ctttagcgac ctgcggcgca
     5461 aaatcggcgg ggtgagcgaa aagatgcttg cgcagtcgtt acaggcgtta gaacaggatg
     5521 gttttcttaa ccgtatcgcg tatccggtgg tgccaccgca tgtggaatat agcctcacgc
     5581 cgttgggcga gcaggtgagc gaaaaggttg ctgcactggc ggactggatt gagttgaatt
     5641 tgcccgaggt gttggcggtg cgggatgaac gtgcggcata actgccggat gcgctgagct
     5701 tatctggtct acggtgcatg gctggtaggg cggataagat gcgccagcat cgcatccgcc
     5761 aataaatgcc ggatgcggtt gataatttat agaccgaaaa agaagtgttt tacttactca
     5821 aatccacctg ataaatcgca aacccgatat catcggtcgc gactttattc atcggatatt
     5881 gccctttctc tttaataaac gctgcggctt tatctgacgg ggaagtttcg aaacggatat
     5941 ccagtttctt atcgccagct atcggtgcta aacgccagtt gttatctgcc gccgggtgaa
     6001 tttctcccgc acgtttcgac tcatcagcaa tccacgctgt cagcaccgag cggttctcat
     6061 ccggtgaagc aaaagcgata tgtctgtcgc ccgtaccggc aaatttgccg ccgtaagcgc
     6121 gatagttatt ggtggcaacg aggaacatag cgttcggatc aatcggcttg ccattaaagg
     6181 tcaggttctt aatccgctct gcattggcat taatcatctg gcactcgccg tcatagcggg
     6241 cgggttgggt aacatcaatc tgataattca caccatcaat aacatcaaag ttataagtgc
     6301 ggaaaccatc ccagttgatg agtgactgtg gtttcgtgct gttaggatca atctggttaa
     6361 actgtccggc ggagcactcc agccactctt tcacctcttt accgctggct ttcactacaa
     6421 tcagcgtatt ggggtagaga taaagatcgg cggcattacg gaaggtcaac tggccttttt
     6481 ccacctccac atagcttgcc gggtcatttt tgcgaccacc aactttaaac ggtgcggcag
     6541 ctgaaagcac cggcagtttt gccagatccg gatcgccctg aatgtaatgt tcgacatacg
     6601 ctttttgcgc gttgttcacg acttgcacgg tcggatcgtc ctgcaccagc gccagatagc
     6661 tatacatatt gtcggcagat ttaccgattg gcttgctgac gaactggcgt gtggcatcgt
     6721 gatcggcttt gagtgtttct accagcttgc tgtcttccgc cgcgagggat tttttattgg
     6781 cgatgtcgta aatcggccgt gcttccgctt tcgcctgcgt cacctgccat ttaccgctgt
     6841 cattactgag ttgtaagtcg accaccccaa gatgatcgcc ccacatgccc ggcattaccg
     6901 ccggaacacc attcagcgtg cctttggtga tatcagcctc ttcgatatca gcaaaatctt
     6961 tacccgggaa aacggcgtga gcatggccaa acataatggc gttaacgccc ggaatttcac
     7021 tgaggtaata aactgagttt tccgccatta ctttatacgg atcggcagac agcccggaat
     7081 gcgccagaac gacaacgaca tcggcacctt tctcgcgcat ttcaggcacg tatttgcgca
     7141 cggtttcggt aatatcattc accgtcactt ttccggataa attagcttta tcccagccca
     7201 tgatttgcgg cggcacgacg ccaatatagc caatcttcag cgtctgtttt tttccgtctt
     7261 tatcgaccac ttcggtatct ttaattaaat acggtgtaaa cattggctgt ttggttctgg
     7321 cgtcaatgac gttggcattt acataaggga atttcgctcc cgccagcgca tttttcaggt
     7381 aatccagacc gtagttaaat tcatgattgc cgagtgtacc gaccgtataa tccagcgtat
     7441 tcagcgcctt ataaaccgga tgaacatcac ctgcttttaa tcctttcgcc gacatgtaat
     7501 cggccagcgg actcccctga atcaaatcgc cgttatcgac cagtacgctg tttttcactt
     7561 cattgcgggc atcgttaatc aggcttgccg tacgtaccag tccgaatttt tccgtggcgg
     7621 tgtctttgta ataatcgaaa tccatcatgt tgctatgcag atcagtggtt tccatgatac
     7681 gcagatcgac cgtcgctgca ttcacactgg cggcaatcag cgtggccagg agcgttgcgc
     7741 taaacttaat catcagggac atccttttat catcgggaat acgaaagaaa agggagaata
     7801 aacgtcttac ttatagaaca gtgaagaatg ccacaatttt acgctttgaa aatgatgaca
     7861 ctatcacagt tggcgcattc attaacgata gggtataagt aaaacaataa gttaacaccg
     7921 ctcacagaga cgaggtggag aaatgttaga tcaagtatgc cagcttgcac ggaatgcagg
     7981 cgatgccatt atgcaggtct acgacgggac gaaaccgatg gacgtcgtca gcaaagcgga
     8041 caattctccg gtaacggcag cggatattgc cgcacacacc gttatcatgg acggtttacg
     8101 tacgctgaca ccggatattc cggtcctttc tgaagaagat cctcccggtt gggaagtccg
     8161 tcagcactgg cagcgttact ggctggtaga cccgctggat ggtactaaag agtttattaa
     8221 acgcaatggc gaattcaccg ttaacattgc gctcattgac catggcaaac cgattttagg
     8281 tgtggtgtat gcgccggtaa tgaacgtaat gtacagcgcg gcagaaggca aggcctggaa
     8341 agaagagtgc ggtgtgcgca agcagattca ggtccgcgat gcgcgcccgc cgctggtggt
     8401 tatcagccgt tcccatgcag atgcggagct gaaagagtat ctgcaacagc ttggcgaaca
     8461 tcagaccacg tccatcggct cttcgctgaa attctgcctg gtggcggaag gacaggcgca
     8521 gctgtacccg cgcttcggac caacgaatat ttgggacacc gccgctggac atgctgtggc
     8581 cgcagctgcc ggagcgcacg ttcacgactg gcagggtaaa ccgctggatt acactccgcg
     8641 cgagtcgttc ctgaatcctg ggttcagagt gtctatttac taaattcaga tggcagaaac
     8701 agtgtatttc ctgattctgc catcctgatt tctcccaacc taaaaagtta taaataaaaa
     8761 gagattgtat ttaaagtgca aaaattcaat tgctaataag ttacatttta ataatgagcg
     8821 ttttttgata gtttacttct ataatgagat atttaatggc gacataaaat aaccaaataa
     8881 aataaggtag ccatatgtta cccaggatca gacacaataa ttttattggt gcggtggagt
     8941 tattcgtaaa atcatcgcat acaaaaacac attcaaacaa tttttttaat aatattcagc
     9001 atgcatttaa gaaaaaagat tgggtttcga attatgatag cctgttaact ttgtgggagt
     9061 tttttcgttg cgcaactcag attgataaag atagttatca agtgctttca tcaaaaaatg
     9121 aaactgttaa agccatggat aagtttctta tcagtttcag ccttaaagat aacggtgccg
     9181 aatatacaat gacattgcga ggcagtggat ttgaatacga agaaatcccc ataacaataa
     9241 atgagtataa ctcattcatg gatttcaaaa accgtgaatt tccgttagaa caaaacagaa
     9301 gattgtatgc ttgggatatt ctgcagaaaa aacagtctga cataccgaaa aggattaaag
     9361 gttatattca ccaggcgatt ggtgacgtgt ctttaggtta tgccttattg gaggatatag
     9421 taagcaaatt aaaacggggt aaatttgaac tccaaatacc aggaggtgga attaaagaat
     9481 gcgatggatg gtatatttac gaaaaaataa ttgatgataa ttttgccata gtcatagaat
     9541 ctttgggctt tgcgcttaaa atctatggag gggatgaacg ttttcgtaat ggttcctctg
     9601 ttgttttgga ggatgaagat tactctctta tttataattt tctggttaat gcaggttgcc
     9661 aacaagtcga attagccgaa caagtggatg cgatagtaag tgcgaatctg gccgtagata
     9721 gtgatattac cgaagaggag atttgcgaga aatataaatc aaccattgag gcgtttaaaa
     9781 aagag
//
LOCUS       DAJTNN010000155         9722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTNN010000155 DAJTNN010000000
VERSION     DAJTNN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.155"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            149..301
                     /gene="hokA"
                     /locus_tag="OO150_003311"
     CDS             149..301
                     /gene="hokA"
                     /locus_tag="OO150_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5517349.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(489..701)
                     /gene="cspA"
                     /locus_tag="OO150_003312"
     CDS             complement(489..701)
                     /gene="cspA"
                     /locus_tag="OO150_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5517350.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(982..1272)
                     /locus_tag="OO150_003313"
     CDS             complement(982..1272)
                     /locus_tag="OO150_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR5517351.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1705..2415
                     /locus_tag="OO150_003314"
     CDS             1705..2415
                     /locus_tag="OO150_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5517352.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2465..3439)
                     /gene="ghrB"
                     /locus_tag="OO150_003315"
     CDS             complement(2465..3439)
                     /gene="ghrB"
                     /locus_tag="OO150_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5517353.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKPSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3543..4202)
                     /locus_tag="OO150_003316"
     CDS             complement(3543..4202)
                     /locus_tag="OO150_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5517354.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4355..6688
                     /gene="bisC"
                     /locus_tag="OO150_003317"
     CDS             4355..6688
                     /gene="bisC"
                     /locus_tag="OO150_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5517355.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVHVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVHAAKIRELAALFHQNTTMLMAGWGMQRQQLGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRCSAVLSSMQGSLPGGSDAVDK
                     IPVARIVEALENPGCAYQHNGMNRLFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDALARGIQD
                     GDTVRLWNARGQILAGVVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6657..7097)
                     /locus_tag="OO150_003318"
     CDS             complement(6657..7097)
                     /locus_tag="OO150_003318"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5517356.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7094..7657)
                     /gene="tag"
                     /locus_tag="OO150_003319"
     CDS             complement(7094..7657)
                     /gene="tag"
                     /locus_tag="OO150_003319"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5517357.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7815..8513
                     /locus_tag="OO150_003320"
     CDS             7815..8513
                     /locus_tag="OO150_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5517358.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8741..>9722
                     /locus_tag="OO150_003321"
     CDS             8741..>9722
                     /locus_tag="OO150_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5517359.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPS"
BASE COUNT         2316 a         2364 c         2510 g         2532 t
ORIGIN      
        1 ggtgcttgag actgtttgtc tcaggtattc accgaaaggc agacagagaa aagccccacc
       61 tgactataaa tcaaagtgag gctaccctat gcctgaacac cataaggtta gcctcttact
      121 cgttggaaat caacacaggg ggctgggaat gccgcaaaaa tacggattac tttcattaat
      181 agtgatttgt ttcacgcttt tatttttcac ctggatggta agagattcac tgtgtgaatt
      241 gcatattaaa caggggcgtt atgagctggc ggcattttta gcctgcaatt taaaagagta
      301 agagtcttcg gcgggaaatt attcccgcct ttcttacggc gttgcgcatt ctcattgcac
      361 ccacatttat tcttcacaaa aataataata gattttacta cgcgatcgat tatttatttc
      421 ctgaaaaaaa ataaaaaaaa tccccgccaa atggcaggga tcttagattc tgtgctttta
      481 agcagagatt acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg
      541 gtgaaggaca ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag
      601 aagtgtacga acacatcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca
      661 gcgttgaacc attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa
      721 ttaagtgtgc ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt
      781 gcactacgag gggtatcaac gataactctt gaagggactt gccttactac actgctttaa
      841 tggtctgtac gtcaaaccgt tgatgtgcat taagccacgc attggcgggt gatgcaacaa
      901 ttatttttca tatttatgat taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac
      961 agcaataaag tggataaaag tctattccat caactgctta cttaatgccg ggttggcttg
     1021 aatcaaacgc atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc
     1081 ctttaccatg gcgactgata tgcccaaaac ccgggcgaaa tcatcgattt ttaaccctgt
     1141 cccttttcgt aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt
     1201 ctgcgtttca tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc
     1261 tttatattcc attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct
     1321 cattaagaat agtcgggaaa agcagacaca gcggggtgaa gtcagggcat gctctggcta
     1381 tgattcgatt cagccagtca tttagcgtga cttatagcca cctaacgatc tgataaatgc
     1441 cgatatatct cttaccactc ctgttttgta aagatttcga aaatctaaag ttctgcgaag
     1501 gcgtaaatct cgggctacaa aaaagcaacg gcaatatcat tttttaacaa tgaaacaaca
     1561 aattaacctt gtttcatttt ctccatgatt ttaccaggaa gattacgtag aaagggtatc
     1621 ctgcaagcct gtcctggact atccttgtca cgtcagacac gcgtgtgtcg ttgtgcgctt
     1681 tttttgggtg aaaggagtaa gaaaatggcg acaggaaagt cctgctctcg ctggtttgcg
     1741 cctcttgcgg cgttattaat ggtagttagc ctgagtgggt gttttgataa agaaggcgat
     1801 cagcgtaaag cgtttatcga cttcctgcag aatacagtga tgcgtagcgg tgaacgtcta
     1861 ccaaccctga ctgccgatca gaaaaaacag tttggtcctt ttgtctctga ttacgcgatt
     1921 ctgtatggtt attctcagca ggtgaatcag gcgatggatt ccggtctgcg cccggtcgta
     1981 gacagcgtta acgcaattcg cgtaccgcag gattacgtta cgcaaagcgg tccgctgcgt
     2041 gaaatgaacg gttcgctggg tgtgctggcg caacaactgc aaaatgcgaa gttgcaggcc
     2101 gatgctgcgc actctgcgct aaaacagacc gatgatttga aaccggtctt tgatcaggca
     2161 ttcaccaaag tggtaacgac gccagctgat gcattgcaac cgttgattcc ggttgcacaa
     2221 actttcacgc aacaactggt gatggttgga gactacattg ctcaacaagg tactcaggtg
     2281 agctttgtag caaatggtat ccagttcccg acttcgcagc aggcaagtga atataacaaa
     2341 ctgattgcgc cgttaccggc acaacatcag gcgtttaatc aggcctggac tacagcagtt
     2401 actgcaaccc aataaagagt aaaagcccga gcgattatcg ggctttactt cacgcagtcg
     2461 cggcttagtc cgcgacgtgc ggattcacac agttcttctc aacctttcct tgtaacgcat
     2521 caatcaaatt atccacggca caggcggcca tgccataacg cgtctcatgg gtggcagatc
     2581 caatatgcgg tactgcgacg acgttggcca ttgagagcaa cggcgaatct acggacagtg
     2641 gctcttgttc gaagacatcc agcccggcgg cgtgaatttc gcctttctgc aatgctgcga
     2701 tcagtgcatt ttcgtcaacc accgggccac gtccggcatt aatgaaaatg gcggagggtt
     2761 tcattttggc gaattgttct gcgccaaaca gatgatgcgt ctcatcagtt aacggcagga
     2821 tcaggcaaac gaaatctgac tcttgtaaca gagtatccaa atcgcagtag cgggcgttga
     2881 agcgttcttc tgcttcttta tggtggcggc gcgcgttata gaggatgggc atgttgaagc
     2941 caaagtgcgc acgttgtgcc agcgccatgc cgatccgtcc catcccgaca atgcccagtg
     3001 ttttatggtg aacgtcagtg ccgtaccagt ccgggcctat gctcgcggtc cattcgcctg
     3061 cttttacccg ttctgctacc tccacaaccc gacgagcggt agacaacacc agcgccatca
     3121 gcgtatcggc gacggtttct gttaatacgg ttggcgtgtg catcagcaga atttttcggg
     3181 cggtaagcgc atcgacatca aaattgtcat agccgacgga gatcgttgat gtggcacgca
     3241 gtttcggcat tttttccagc aatgcggcat ctacattctc gtttgaaccc agtaaacctt
     3301 cagcttcggc aaaaattgct gcattttgtt cgacggtttg tgggctgagg tttgccacct
     3361 ggtgaacggt gaaatgctct tgcaggcgtt gcagtaaatc atcaggtaag gctttgtaga
     3421 ggataacgga cggcttcatg cttctctcca ttcactgata aggcgctcac cttagcctga
     3481 aatatgatga aggaaaactg aagaatgcca ggtgatttct gcatcacctg gcatgaaagg
     3541 gattacagcg ggcttaaggt aatttctaca cggcggtttt gcgccttacc ttctgcggtg
     3601 ctgttgctgg cgattgggtt agccgggcca aggccctgag tacggatgcg gctggcgtcc
     3661 acgccctggg tgatcaacgc gctggcaacg gaatccgcac gctgctggga gagacgcatg
     3721 ttcaggtcgt gaccccccgt gctgtcggta taaccaatca cgttaaccgc cgttttcgga
     3781 tactctttca gcaccattgc cacgccggtc agggtgttag cgcctgccgg tttcagggtc
     3841 gcgctgctgc tgtcgaaggt cacattgttc ggcatattga ggataatgtt atccccgctg
     3901 cgggttacgc taacaccagt gccgcgcatt ttgtcacgca gcttcgcttc ctgcacatcc
     3961 atgtaataac ccacgccgcc gcccagagct gcgcctgctg ctgcgccaat cagcgcgcct
     4021 ttaccgcgat ctttcttcga agaagagagc gcaccaatac ccgcgcccac gagagagtcc
     4081 agacctgcgc cgatagcaga tttgcctgct tcgcgttcgc cggtgtaagg gttagttgtg
     4141 cagccagata ccgccagagc gccactcact acggcggcaa taagataaac acgtttcttc
     4201 attgttaatc cttaataacc tttttattct ttgccacggg ttccgtggcg ggagattatg
     4261 ccgcgtgaac atgaagatta ttcctgggaa tactcggaaa tttgtaagta atatttaact
     4321 gctcaataca tctaaccttt caggagtctt cggtttggcc aactcatcct cacgatattc
     4381 cgttctgact gccgcccact gggggcccat gctggttgaa accgacggcg aaaccgtgtt
     4441 tagctcgcgt ggcgcgttag ccacaggaat ggaaaactcc ttgcagagcg cggttcgcga
     4501 ccaggttcac agcaatacgc gggtacgatt tccaatggtg cgcaaaggct ttctcgcgtc
     4561 accggaaaac ccgcaaggca ttcgtgggca ggatgaattt gttcacgtga gttgggatga
     4621 ggcgctggat cttattcacc aacaacataa acgcattcgt gaggcttatg gtccggcatc
     4681 gatttttgct ggttcctacg gctggcgttc aaacggcgtg ctgcataagg cctcgacatt
     4741 attacaacgc tatatggcgc tggcaggcgg ttataccggg catctggggg attattcgac
     4801 cggtgcggca caggcgatca tgccgtatgt cgtgggtggc agcgaagttt atcaacagca
     4861 aaccagttgg ccgctggtgc tggaacatag cgatgtcgtg gtgttgtgga gtgctaaccc
     4921 actcaatacg ctgaaaattg cgtggaatgc atctgatgag caggggcttt cttacttttc
     4981 cgcactgcgt gacagcggga aaaagctgat ctgtattgat ccaatgcgat cggaaaccgt
     5041 cgatttcttt ggcgataaaa tggagtgggt ggcaccgcac atgggcaccg atgttgcgct
     5101 gatgctgggg atcgcccata cgctggtgga aaatggttgg cacgacgaag cgtttctggc
     5161 gcgttgcacc acaggttatg ccgtcttcgc ctcttatttg ctgggcgaga gtgacggaat
     5221 agccaaaacc gccgaatggg cggcagggat ttgtggggtt cacgcagcga aaattcgcga
     5281 gttggcggct cttttccacc aaaacacgac catgctgatg gctggctggg gaatgcagcg
     5341 ccaacagctt ggtgagcaaa aacactggat gatcgtcacg ctggcggcaa tgttggggca
     5401 aatcggcaca cccggcggcg gttttggtct ttcttatcat tttgccaatg gtggtaatcc
     5461 cacgcggtgt tctgcggtgc tctcttccat gcagggtagc ttgccgggtg gcagcgatgc
     5521 ggtagataaa atccctgttg cccgtattgt tgaagcactg gaaaaccctg gctgcgcata
     5581 ccaacacaat ggcatgaatc gcctcttccc ggatattcgc tttatctggt gggcgggtgg
     5641 cgccaacttc acccatcatc aggacactaa tcgcctgata cgagcttggc aaaaaccgga
     5701 gctggtagtt atctccgaat gcttctggac ggcggcggca aaacacgcgg atatcgttct
     5761 gcctgcgact acctcttttg agcgtaatga tctcaccatg accggcgatt acagtaatca
     5821 gcatctggtg ccgatgaagc aagtggtgcc gccacgctat gaagcgcgta atgattttga
     5881 tgtttttgcc gagttaagtg aacgctggga gaagggcggt tatgcacgtt ttacggaagg
     5941 aaaaagtgag ttgcaatggc tggaaacgtt ttataacgtt gcccgacagc gcggggcaag
     6001 ccagcaggtt gaattgccgc catttgctga gttctggcaa gccaatcagt taattgagat
     6061 gccggaaaac ccggacagcg agcggtttat tcgcttcgct gatttttgcc gcgatccgca
     6121 ggcgcatcca ttaaaaaccg ccagcggtaa gattgaaatc ttctcgcagc gtattgccga
     6181 ttacgcttat ccggattgcc cggggcatcc aatgtggctg gagccggacg aatggcaggg
     6241 caatgccgaa ccggaacagt tacaggtact ttctgctcat ccggcgcatc gtctgcacag
     6301 ccagctgaat tacagttctc tgcgcgaatt gtatgcggtg gcaaatcgtg agcctgtcac
     6361 cattcatcct gacgatgccc tggcgcgcgg cattcaagat ggcgatactg ttcggctgtg
     6421 gaacgcacgc gggcaaattc ttgccggagt ggtcattagc gagggaatta aacctggcgt
     6481 gatttgtatt cacgaagggg catggccgga tttggattta accgctgacg gtatttgtaa
     6541 aaacggcgca gtcaacgtgc tgaccaaaga tctccccagc tcgcggctgg ggaatggctg
     6601 tgcgggtaat acggcgctgg catggctgga aaaatacaac ggtccggaac tgacacttac
     6661 agcgtttgaa ccaccggcca gctcataatc caggtgggta gttgggtttc atcctgccat
     6721 gcgcaatcga caatgtgaaa accctgcgcc tggtaaaaat ttatcgccgg ttgatttttt
     6781 tgataaacct ccagcatcag gtggggatgg cgctgctgca catattgcat cagcgcctta
     6841 ccaataccgc gcctgacggc cttcggtgcg acaaacatcg ctgccagaaa tcggccttcc
     6901 ataatgctga caaaaccgag aagcttactg tcttcttccc agacccagtt ttgcgcattg
     6961 gcaagatacg catcccgcac cagcggaatg caatcacgcc agtaattcgc ttttataaag
     7021 ggatgtcccc aggttgtact ttccagccac agttcgagga tcgcggggag ttctgaacgt
     7081 tgcgcttccc gaatcatggt ttatttcccg gatagcaaca gcagccaacc acatgatcat
     7141 tcaccagccc acatgcctgc ataaaggagt aacagattgt ggtaccgaca aacttaaaac
     7201 cacgtttttt cagtgcttta gatagggcgt cggaggcggg cgtagatgtg gggatttcgc
     7261 tcaacgttgt ggcttgtgtc acctgtggct gatgatttac aaacgaccag acaaagtcgg
     7321 caaacggttc gccgttctgt tccatttgca ggtacgcccg cgcattacca ataattgcct
     7381 gaattttccc gcgatggcgg ataatcccgg cgtcctgtac cagtctttcg acatcctctt
     7441 cctgcattgc tgcgaccttc accggatcga actgatgaaa gcaggcgcga tagttttcgc
     7501 gttttttgag gacggtgatc cacgataacc cagcctgctg cccttcaagg cagatcattt
     7561 cgaacagttt tttactgtca gtttcaggca cgccccactc attatcatgg taggcaatat
     7621 aaagcgggtc ctgactcacc cagccgcaac gttccatact ttccctcgct atgtacccga
     7681 tcgtaaaaat ttcagccgct tttcttgacg catcggtgaa aaagtcaata tttaatacag
     7741 ggctactatg cccgatatcc ttcatacaat gacaataata ttgccgaatt cggctcttcc
     7801 ctggagtcgt gttaatgatc ataaaaaaaa gcggtggtcg ctggcagcta agcctgctgg
     7861 cgagcgtggt aatcagcgcc ttttttctca acacagctta cgcctggcaa caagaatata
     7921 tcgttgatac gcaacccgga cattccacag agcgttacac ctgggatagt gatcatcaac
     7981 ctgattacaa cgatattttg tcgcaacgta ttcaaagtag ccaaagggcg ctgggactgg
     8041 aagtcaatct ggcggaagaa actcctgtgg atgtgaccag cagtatgagt atgggctgga
     8101 attttccttt atatgaacag gttacaaccg gcccggtcgc ggcattacat tacgatggca
     8161 caaccacctc gatgtataac gagtttggcg acagtactac cacgctgacc gatccgttat
     8221 ggcatgccag cgtgagtagc ttaggctggc gtgttgactc ccggcttggc gatctccgac
     8281 cctgggcgca aatcagctat aaccagcaat ttggcgagaa tatctggaag gcgcaatcag
     8341 gcctgagccg gatgacggcg acaaaccaga acggcaactg gctggatgtc accgtaggcg
     8401 ctgatatgtt gctcaatcaa aatattgccg cctatgccgc gctaactcag gcagaaaata
     8461 ccactaataa tagcgactat ctgtatacga tgggggttag cgccagattt taacgtaaca
     8521 gtcacaattg aaaccattga ataacaatag ttgtggtgat agtgggtgct aacttacaaa
     8581 taataaattt ggtgaataat tgtcgcgtca ttcattcctg aactaaggca tttcattccg
     8641 ttctgatggc atttcatgcc gtttttcccc aggcataaaa tgcacttcgt tatggttgtc
     8701 ggcagagatt tttccttttt attactgcag gaatactgcc atgacacctt caaattatca
     8761 gcgtacccgc tggctgacac tcatcggtac tatcattacc cagtttgcgc tggggtcggt
     8821 ttatacctgg agcctgttta atggcgcgct ttctgccaag ctggatgcgc cggtaagcca
     8881 ggtcgctttc tctttcggct tgttaagtct ggggctggca atttcatctt ctgttgcggg
     8941 gaaattacag gaacgttttg gcgttaaacg cgtcactatg gcttccggca ttttgctagg
     9001 attaggcttc ttcctgacag cgcattctga caacctgatg atgctgtggt taagcgccgg
     9061 tgtgctggtc ggtctggcgg atggcgcggg ttacctgctg acgctctcta actgcgtgaa
     9121 gtggttcccg gagcgtaaag gtctgatctc cgcgttcgct atcggttctt atggtcttgg
     9181 tagcctgggt ttcaaattta tcgacacgca gctgctggaa acggtgggtc tggaaaaaac
     9241 atttgtgatt tggggagcga ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa
     9301 agacgcacca aaacaggaag tgaaaaccag caatggtgtg gtggagaaag attacacgct
     9361 ggcagagtcg atgcgtaaac cgcagtactg gatgttagcg gtaatgttcc tgactgcctg
     9421 catgagcggc ctgtacgtga ttggggtagc gaaagatatc gcccaaagtc tggcacacct
     9481 tgatgtggtt tccgcagcca atgcagtaac ggttatttcc atcgctaacc tttcaggtcg
     9541 tctggtgctg ggcattctgt ctgacaaaat tgcccgtatc cgtgttatca ccattggtca
     9601 ggtgatttcg ctggtgggta tggcggccct gctgtttgca ccattaaatg cagtgacgtt
     9661 ctttgcagcg attgcctgcg tggcatttaa ctttggcggc actattaccg tctttccatc
     9721 ac
//
LOCUS       DAJTNN010000156         9706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTNN010000156 DAJTNN010000000
VERSION     DAJTNN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.156"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            48..731
                     /locus_tag="OO150_003322"
     CDS             48..731
                     /locus_tag="OO150_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5517360.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            775..5625
                     /gene="ehaG"
                     /locus_tag="OO150_003323"
     CDS             775..5625
                     /gene="ehaG"
                     /locus_tag="OO150_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HCR5517361.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALDGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSQVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            5993..7648
                     /gene="lldP"
                     /locus_tag="OO150_003324"
     CDS             5993..7648
                     /gene="lldP"
                     /locus_tag="OO150_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5517362.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7648..8424
                     /gene="lldR"
                     /locus_tag="OO150_003325"
     CDS             7648..8424
                     /gene="lldR"
                     /locus_tag="OO150_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5517363.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8421..9611
                     /gene="lldD"
                     /locus_tag="OO150_003326"
     CDS             8421..9611
                     /gene="lldD"
                     /locus_tag="OO150_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5517364.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADTHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         2473 a         2440 c         2507 g         2286 t
ORIGIN      
        1 aactctaccg gcattgatcg ctggtcggag gaaataagga tttttacatg aacttgaaga
       61 agattttttt cagtgctgtc actgtttcag ttctctgcgc attaacggga tgtgactaca
      121 ttgaagaagg caagccagaa agtagtttat tgaaacaaca agaagaacat aacaataaaa
      181 ttgatctact tgaaaaacaa caagcacagc taaagagtca actggaaact attcaaaaac
      241 aacaaactgg cataataagc agtacaaaga ctttaactca cgtaattaaa tccgttaaag
      301 atcaacaaaa cacttttatt tttaccgagt ttaacccagc aaaaacgaag tatttcatcc
      361 tgaacaatgg ttcggtggct ttagcgggtc gagtgttatc cattgacgcc acagagaatg
      421 gcagtgttat tcatatttca ctggtcaact tattaagtac acctatctca aatattggtt
      481 ttaatgcgac atggggtggt gaaaaacctg tcgatgccaa agagtttgcc cggtggcaac
      541 aattactttt caacacatca atgaaatcca cattgaaatt attaccaggt caatggcaag
      601 acattaattt gaccctgaag ggtgtctcgc ccaataacct gggatatctg aagttagcca
      661 tcaacatgga aaatattcag ttcgacaatc ttccctctgc tgaaaatcgg cagaaaagaa
      721 gcaaaaaata aatgcagtta gtacacttat aacaaggaat agcgagcatc attaatgaac
      781 aaaatattta aagttatctg gaaccctgcg acagggaatt atactgttac cagcgaaacg
      841 gcaaaaagcc gtggcaagaa atctgggcgc agtaagctgt taatttctgc gctggttgcg
      901 ggtggaatgt tgtcgtcgtt tggggcattg gcgaatgccg ggaatgacaa cggtcagggt
      961 gttgattacg gtagtggatc agctggcgac ggctgggttg ctataggcaa aggggcgaaa
     1021 gcaaatactt ttatgaacac cagtggttcc agtactgctg tgggttatga cgctatagct
     1081 gaaggccaat atagctctgc catcgggtca aaaacccatg cgattggtgg tgcatcaatg
     1141 gcctttgggg ttagtgcaat atcagaaggc gatagaagta tagcactggg tgcctcttcg
     1201 tattcattgg gccaatactc aatggccctc ggccgttatt caaaagcatt gggtaaattg
     1261 tctattgcta tgggggactc ttccaaagcg gaaggagcaa acgccattgc cctgggaaat
     1321 gccactaaag ctactgagat tatgagtatt gctcttggcg acaccgccaa tgcgtcaaaa
     1381 gcgtattcaa tggcgctggg agcaagtagc gtcgcatctg aagaaaacgc aattgccctg
     1441 gggcgtagca gtgtagctag cggtactgac agcctcgcat ttggcagaca atcacttgcc
     1501 agcgcagcga acgctattgc gataggtgct gagaccgaag ccgctgaaaa tgcaactgct
     1561 attggcaata atgcgaaggc aaaagggact aatagcatgg caatggggtt cggaagcctt
     1621 gccgataaag tcaatactat cgcattagga aatggcagcc aggctctggc agataatgca
     1681 atcgccatag gccagggcaa caaagctgat ggcgtggatg ccatcgctct gggtaatggt
     1741 agccagtcga gaggcttaaa caccattgcc ttaggcacag ccagtaatgc aactggtgat
     1801 aagagtcttg cgcttggtag taatagcagt gccaacggta ttaactctgt cgcgctgggc
     1861 gcagattcca ttgcggattt agacaatacc gtctctgtcg gcaatagttc attaaaacgc
     1921 aagatcgtta atgtgaaaaa tggcgcgatc aagtctgaca gttacgatgc cattaatggt
     1981 tcacaacttt atgccattag cgactcggta gcaaaaaggc ttggaggagg ggctgcagta
     2041 gatgttgatg acggtactgt tacagcacca acctacaatt taaaaaatgg tagcaaaaat
     2101 aacgtagggg ctgcgctcgc tgtacttgat gaaaacaccc tgcaatggga ccaaaccaaa
     2161 ggcaaataca gcgctgctca tggtactagt agcccaactg ccagcgtaat caccgatgtt
     2221 gcggatggca cgatttcagc ctccagtaag gatgcggtta acggttccca actgaaagct
     2281 accaatgacg atgtcgaagc caacaccgcc aatatcgcta ctaataccag caacattgcc
     2341 acgaatacgg caaatattgc caccaatacc accaatatca ccaacctgac ggattccgtt
     2401 ggtgaccttc aggctgatgc cctgctctgg aacgaaacta aaaaggcatt cagtgcagct
     2461 cacggccagg ataccaccag caaaatcacc aacgttaaag atgccgacct gacggctgac
     2521 agcactgatg ctgttaacgg ctctcagctg aaaaccacca acgatgctgt ggcgacgaat
     2581 accaccaata tcgccaataa cacttccaat attgccacta acaccaccaa catctctaat
     2641 ctgactgaga cggtgactaa tcttggtgag gatgcgctga aatgggataa ggacaatggt
     2701 gtattcacgg cagctcatgg caccgagacc accagcaaaa tcaccaacgt taaagatggc
     2761 gacctgacga ctggcagcac cgatgccgtt aacggctctc agctgaaaac caccaacgat
     2821 gccgtggcga cgaataccac caatatcgcc actaacacca ccaacatctc taatctgact
     2881 gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtcttc
     2941 actgcagctc atggcaacaa taccgccagc aaaatcacca atatcctgga cggcacagtc
     3001 actgcaacca gttccgatgc cattaacggt agccagcttt atgacttaag cagcaatatc
     3061 gccacctact tcggcggcaa tgcttctgtg aatactgacg gtgtgtttac cggtccaacc
     3121 tacaaaatcg gtgaaacaaa ttattataac gtcggcgatg cactggctgc gattaactcc
     3181 tcatttagca cgtctctcgg cgatgctctg ctttgggatg ccaccgcagg taaattcagt
     3241 gccaaacacg gtactaatgg tgacgcaagc gtgatcactg atgtcgcaga tggtgaaatt
     3301 tcagactcca gttctgacgc agtaaacggc tcacaactcc acggcgtgag cagttatgtt
     3361 gttgatgcgc tggggggtgg tgccgaagtc aatgcagacg gcaccatcac tgcgccgacg
     3421 tacaccattg ctaatgctga ttacgataat gtcggtgatg ccctgaatgc tatcgatacc
     3481 actcttgacg acgctctgct ctgggatgcg gacgccggtg aaaatggtgc atttagcgcc
     3541 gctcacggaa aagataaaac tgccagtgta atcactaacg tcgctaacgg tgcaatctct
     3601 gctgccagca gcgacgcgat taacggctca caactctata ccaccaataa gtacatcgct
     3661 gatgcgctgg atggtgacgc agaagtcaac gctgacggca ccatcaccgc accgacttac
     3721 accattgcga acgccgagta caacaacgtc ggtgacgctc tggatgcgct tgatgataac
     3781 gccctgctgt gggatgagac tgccaatggc ggtgctggag cctacaatgc cagccatgac
     3841 ggtaaagcca gcatcatcac taatgtcgct aatggcagta ttagtgagga cagtaccgat
     3901 gcagtgaacg gttctcagtt gaatgcgacg aatatgatga ttgagcagaa cacccaaatt
     3961 atcaatcagc tcgctggtaa caccgacgca acctatatcc aagaaaacgg tgcgggtatt
     4021 aactatgtgc gtactaacga cgacggctta gcgttcaacg acgccagcgc acagggtgtt
     4081 ggcgctacag ctataggtta taactctgtc gccaaaggcg atagcagcgt agctattggt
     4141 cagggcagct acagcgacgt tgatacgggt atcgccctag gtagcagctc tgtttccagc
     4201 caagtgattg ccaaaggctc ccgtgacacc agcataacgg aaaatggcgt tgttattggt
     4261 tacgacacca cggatggcga actgctcggt gcattgtcta tcggtgatga cggtaaatat
     4321 cgtcaaatca tcaacgtagc cgatggttcc gaagcccatg acgccgttac ggttcgtcaa
     4381 ttgcagaatg cgattggtgc ggtcgcaacc acgccgacta aatacttcca cgctaattca
     4441 acggaagaag attcactggc agtgggaact gactcgctgg caatgggtgc gaaaaccatc
     4501 gtgaatggcg ataaaggtat tggtatcggt tatggtgcct acgtggacgc gaatgcactt
     4561 aacggcattg ccattggtag caatgcgcaa gtcattcatg tcaacagtat tgcgataggt
     4621 aatggttcta cgaccactcg tggcgctcaa accaattata ccgcctacaa catggacgca
     4681 ccgcagaact ctgtcggtga attctcagtc ggtagtgcgg atggtcaacg tcagatcaca
     4741 aacgtcgcag ctggttcagc ggataccgat gcggttaacg tgggtcagtt gaaagtcact
     4801 gatgagcgcg tagcgcaaaa tacccagagc attactaacc tgaacaatca ggtcactaat
     4861 ctggatactc gcgttactaa tatcgaaaac ggtattggcg acattgtcac caccggtagc
     4921 accaagtact tcaagaccaa caccgatggc gtagatgcca acgcccaggg taaagatagc
     4981 gttgctattg gttctggttc cattgctgcc gctgacaaca gcgtcgcact gggtaccggt
     5041 tccgttgcag aggaagaaaa tacaatctct gtaggttctt ctactaacca acgccgtatc
     5101 accaacgtag ctgccggtgt taatgccacc gatgcggtta acgtgtcgca gctgaaatct
     5161 tctgaagcag gcggcgtgcg ctacgacacc aaagctgatg gttctatcga ctatagcaat
     5221 atcaccctcg gtggtggcaa cggcagtacg actcgtatca gcaacgtctc cgctggcgtc
     5281 aacaacaacg acgcggtgaa ctacgcgcag ttgaagcaaa gcgtgcagga aacgaagcaa
     5341 tacaccgatc agcggatggt tgagatggat aacaaactgt ctaaaactga aagcaagttg
     5401 agcggtggta tcgcttctgc aatggcaatg accggtctgc cgcaggctta tacaccgggt
     5461 gccagcatgg cttctattgg tggcggtact tacaacggtg aatcggcagt tgctttaggt
     5521 gtatcgatgg tgagcgccaa tggtcgttgg gtctacaaat tacaaggtag taccaatagc
     5581 cagggtgaat actccgccgc actcggtgcc ggtattcagt ggtaatgatc cattaagtta
     5641 gtgtgactaa gggcaaacgc caaattgcct gatgcgttac gcgcatcagg caagataagg
     5701 cgctccgggt caacaatcta agggcaattc tctgatgagg attgcccttt tctttaccag
     5761 acatctcccc ccacaagaat tggccctacc aattcttcgc ttatctgacc tctggttcac
     5821 aattccccaa ttaaaactca catcaacatt gccaatacac aacatttagt taaccattca
     5881 ttgtcattat ccctacacaa cacaattggc agtgccactt ttacacaacg tgtgacaagg
     5941 agatgagcaa cagactcatt acacgatgtg cgtggactcc aggagacctg caatgaatct
     6001 ctggcaacaa aactacgatc ccgccgggaa tatctggctt tccagtctga tagcatcgct
     6061 tcccatcctg tttttcttct ttgcgctgat taagctcaaa ctgaaaggat acgtcgccgc
     6121 ctcatggacg gtagcaatcg cccttgccgt ggctttgctg ttctataaaa tgccggtcgc
     6181 taacgcgctg gcctcggtgg tttatggctt cttctatggt ttgtggccca tcgcgtggat
     6241 cattattgca gcagtgttcg tctataagat ctcggtgaaa accgggcagt ttgacatcat
     6301 tcgctcgtct attctttcga taacccctga ccagcgtctg caaatgctga tcgtcggttt
     6361 ctgtttcggc gctttccttg aaggagccgc aggctttggc gcaccggtag caattaccgc
     6421 cgcattgctg gtcggcctgg gttttaaacc gctgtacgcc gccggactgt gcctgattgt
     6481 taacaccgcg ccagtggcat ttggcgcgat gggcattcca atcctggttg ccggacaagt
     6541 aacaggcatc gacagttttg agattggcca gatggtgggg cgtcagctac cgtttatgac
     6601 cattatcgtg ctgttctgga tcatggcgat tatggacggc tggcgcggta tcaaagagac
     6661 gtggcctgcg gtcgtggttg cgggcggctc gtttgccatc gctcagtacc ttagctccaa
     6721 cttcattggg ccggagctgc cggacattat ctcttcgctg gtatcactgc tctgcctgac
     6781 gctgttcctc aaacgctggc aaccagttcg cgtattccgc ttcggtgact tgggggcgtc
     6841 acaggttgat atgacgctgg cccacaccgg ttacaccgcg ggtcaggtgt tacgtgcctg
     6901 gacaccgttc ctgttcctga ccgctaccgt aacactgtgg agcatcccgc cgtttaaagc
     6961 cctgttcgca tcgggtggtg cgctgtatga gtgggtgatc aacattccgg tgccgtacct
     7021 cgataaactg gttgcccgta tgccaccagt ggtcagcgag gctacagcct atgccgccgt
     7081 gtttaagttt gactggttct ctgccaccgg caccgccatt ctgtttgctg cactgctctc
     7141 gattgtctgg ctgaagatga aaccgtctga cgctatcagc accttcggca gcacgctgaa
     7201 agaactggct ctacccatct actccatcgg catggtgctg gcgttcgcct ttatctcgaa
     7261 ctattccgga ctgtcatcaa cactggcact ggcactggcg cacaccggtc atgcattcac
     7321 cttcttctcg ccgttcctcg gctggctggg ggtattcctg accgggtcgg atacctcatc
     7381 taacgccctg ttcgccgcgc tgcaagccac cgcagcacaa caaattggcg tctctgatct
     7441 gttgctggtt gccgccaata ccaccggtgg cgtcaccggt aagatgatct ccccgcaatc
     7501 tatcgctatc gcctgtgcgg cggtaggcct ggtgggcaaa gagtctgatt tgttccgctt
     7561 tactgtcaaa cacagcctga tcttcacctg tatggtgggc gtgatcacca cgcttcaggc
     7621 ttatgtctta acgtggatga ttccttaatg attgttttac ccagacgcct gtcagacgag
     7681 gttgccgatc gtgtgcgggc gctgattgat gaaaaaaacc tggaagcggg catgaagttg
     7741 cccgctgagc gccaactggc gatgcaactc ggcgtatcac gtaattcact acgcgaggcg
     7801 ctggcaaaac tagtgagcga aggcgtgctg ctcagtcgac gcggcggcgg gacgtttatt
     7861 cgctggcgtc atgacacatg gtcggagcaa aacatcgtcc agccgctaaa aacgctgatg
     7921 gccgatgatc cggattacag tttcgatatt ctggaagccc gctacgccat tgaagccagc
     7981 accgcatggc atgcggcaat gcgcgccaca cctggcgaca aagaaaagat tcagctttgc
     8041 tttgaagcaa cgctaagtga agacccggat atcgcctcac aagcggacgt tcgttttcat
     8101 ctggcgattg ccgaagcctc acataacatc gtgctgctgc aaaccatgcg cggtttcttc
     8161 gatgtcctgc aatcctcagt gaagcatagc cgtcagcgga tgtatctggt gccaccggtt
     8221 ttttcacaac tgaccgaaca acatcaggct gtcattgacg ccatttttgc tggtgatgcc
     8281 gacggggcgc gtaaagcaat gatggcgcac cttagttttg ttcacaccac catgaaacga
     8341 ttcgatgaag atcaggctcg ccacgcacgg attacccgcc tgcccggtga gcataatgag
     8401 cattcgaggg agaaaaacgc atgattattt ccgcagccag cgattatcgc gccgcagcgc
     8461 aacgcattct gccgccgttc ctgttccact atatggatgg tggtgcatat tctgaataca
     8521 cgctgcgccg caacgtggaa gatttgtcag aagtggcact gcgccagcgt attctgaaaa
     8581 acatgtccga cttaagcctg gaaacgacgc tgtttaatga gaaattgtcg atgccggtag
     8641 cactggcacc ggtgggtttg tgtggcatgt atgcgcgtcg tggcgaagtt caggcagcca
     8701 aagcggcgga cacgcatggc attccgttta ctctctcgac ggtttccgtt tgcccgattg
     8761 aagaagtcgc gccagccatc aagcgcccaa tgtggttcca gctttatgta ctgcgcgatc
     8821 gcggctttat gcgtaacgcg ctggagcgag caaaagcagc gggttgttcg acgctggttt
     8881 tcaccgtgga tatgccgaca ccgggcgcac gctaccgtga tgcgcattca ggtatgagcg
     8941 gcccaaatgc ggcaatgcgc cgctacttgc aggcggtgac gcatccgcaa tgggcgtggg
     9001 atgtgggcct gaacggtcgt ccacatgatt taggtaatat ctcagcttat ctcggcaaac
     9061 caaccggact ggaagattac atcggctggc tggggaataa cttcgatccg tccatctcat
     9121 ggaaagacct tgagtggatc cgcgatttct gggatggccc gatggtgatc aaagggatcc
     9181 tcgatccgga agatgcgcgc gatgcagtac gttttggtgc tgatggaatt gtggtttcta
     9241 accacggtgg ccgccagctg gacggtgtac tctcttccgc tcgtgcgttg cccgctattg
     9301 cagatgcggt gaaaggtgat atcgccattc tggcggatag cggaattcgt aacgggcttg
     9361 atgtcgtgcg tatgattgcg ctcggtgccg acaccgtact gctgggtcgt gctttcctgt
     9421 atgcgctggc aacagcgggc caggcgggtg tagctaacct gctaaatctg atcgaaaaag
     9481 agatgaaagt ggcgatgacg ctgactggcg cgaaatcgat cagtgaaatt acgcaagatt
     9541 cgctggtgca agggctgggt aaagagttgc ctgcggcact ggctcccatg gcgaaaggga
     9601 atgcggcata gtcgtttgcg cccccgttac tttattcgta ccccttataa tggggtgtta
     9661 gccagccaga cccggcatga ttactgcccc cagtcgtcca tgatcc
//
LOCUS       DAJTNN010000157         9651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTNN010000157 DAJTNN010000000
VERSION     DAJTNN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.157"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..2093)
                     /gene="fdeC"
                     /locus_tag="OO150_003327"
                     /pseudo
     CDS             complement(1..2093)
                     /gene="fdeC"
                     /locus_tag="OO150_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
     gene            3209..3310
                     /locus_tag="OO150_003328"
     CDS             3209..3310
                     /locus_tag="OO150_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517365.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            3674..3937
                     /locus_tag="OO150_003329"
     CDS             3674..3937
                     /locus_tag="OO150_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR5517366.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            3937..4077
                     /gene="ykgO"
                     /locus_tag="OO150_003330"
     CDS             3937..4077
                     /gene="ykgO"
                     /locus_tag="OO150_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCR5517367.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(4112..4339)
                     /locus_tag="OO150_003331"
                     /pseudo
     CDS             complement(4112..4339)
                     /locus_tag="OO150_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5162..5704
                     /gene="ecpR"
                     /locus_tag="OO150_003332"
                     /gene_synonym="matA"
     CDS             5162..5704
                     /gene="ecpR"
                     /locus_tag="OO150_003332"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCR5517368.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINCRFLRKDIKKDKITDQEMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            5730..6365
                     /gene="ecpA"
                     /locus_tag="OO150_003333"
                     /gene_synonym="matB"
     CDS             5730..6365
                     /gene="ecpA"
                     /locus_tag="OO150_003333"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCR5517369.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSNLVVTSLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFTIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            6423..7091
                     /gene="ecpB"
                     /locus_tag="OO150_003334"
                     /gene_synonym="yagY"
     CDS             6423..7091
                     /gene="ecpB"
                     /locus_tag="OO150_003334"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCR5517370.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            7117..9640
                     /gene="ecpC"
                     /locus_tag="OO150_003335"
                     /gene_synonym="yagX"
                     /pseudo
     CDS             7117..9640
                     /gene="ecpC"
                     /locus_tag="OO150_003335"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
BASE COUNT         2607 a         2373 c         2239 g         2432 t
ORIGIN      
        1 atcaggattc agggtgatgg acgaatgtgc tgcatcaagc ggcccggcaa caaacttcag
       61 cgtttgttgt aatgtgccca gctgatactg ctcgctgaaa gcacgcacac acgcacaaca
      121 acgtttccgg cacgggtcga agaaaccgta gcgctgtaaa cgcctggttt tatctccgaa
      181 attgtaccaa tggtgacacc attagtgtct tgcggaacaa atcgcaagcg gctggcctct
      241 cccgtcaccg ggttaccatc agtatccacc gcagtaagcg tcagcgtgtg ggattgctga
      301 ccatcagcaa ccacatctcc tgagagttca ttcacgctca gagtagactt cgacacatcc
      361 atcatcgtgg cgcgcaattc tgcagtgacg gttttgctca tgccatcaac gctaacagta
      421 atcgttgcct cacctgattg cgttccggta gtgaagacag actgatacac cccagcttca
      481 gtttcagtga actcacccag tgttggcttt gcctgtgatt tagtggcctt cagggaacga
      541 gtcacaatat ttccagccgg tttgaaagtt agttcagtct tgatctgatc tttcatgccc
      601 gtgactggct ggccctcggc gtcgcgcaga gacagcacca gcggtttttg ctcattaccg
      661 ttagcaagca tttgaatacg gctctgaccg tcaagcgtta acgccgtgcg atcggcgctc
      721 ataccagctc cggtaatgac cacctctgtc tgcacgcgtt ttgaggtatt gcctttgtta
      781 tcgtaggcaa ccgcagaaat cgcataataa ttgtctttgc ctggacgata agccgggagc
      841 gttacttgcc actgactacc ctgaccggta attttgccac cttcagccag taatgacggc
      901 gcttcccact gcacattttt cagtccgtga gttgctttgc tgaccacaag cccgagggaa
      961 agtgtctgac agcccttgcc ttcaatacgc tcaggcagag caatacggat cacttcagat
     1021 ttgcggtact caagaacgat gttgttatta cgctcaacca ggtcatagcg gctgcctgct
     1081 agcattcgac gctcgcgaat gctgtctgta tcgagttgtt tctccagagg ttcgccaatc
     1141 cgataattaa cttccaggcc aaagcgagtg tcattctcac cactcttgcc ctgcttatgc
     1201 ccggcactca gggtcagaag aggcacaggc gtgtaattca cttcagcgga aatcgcatgt
     1261 ggatctttct ggcgtttatc tttgccaaac agcccaactt catcgccata atactgctca
     1321 tacatcaggc ttgcgccaag ctgcggtcag gcgggtaaat agccctcagc acgaatatcc
     1381 cagccattcg ccgggcgttc ctgataatcc tcaatatccg gcgatttttt ccagccagaa
     1441 gcccgaatat aaccattggc gctcagtttc agataatcgc gccagtattc cgcaccaaca
     1501 ccaatgcggg tatgactacg ggataaatca tggtcgataa aggtgttcac cccccccatt
     1561 cagtcatttc ctgaaaaatg acgccagcca aaaccaatat ttgactgagt acgatcgtct
     1621 gtacgatgta ttgccccctg agtgaacaac atatttgtcg gcgtatcata aatcggataa
     1681 agcatttcca gcgaagaatc cttcagcgag aaatctttat cgacattcag tttgacgcgc
     1741 gctgtaccat atttcccgag ccactcctgt atttcctggt tagctttagc ggtggccatt
     1801 ccggtaataa aattacgtgt cgcatcgcta tctggctgac tgcttaaaaa tgtcccggca
     1861 tttgcggcaa acgacgcgac atttttctcc acgttattat cagcagttac cgtagtattt
     1921 cccatgctca accgtggctg aaccgcatgc tgcgcacgtg ccgccattac tggggtaaag
     1981 gtgacagcga gtggaaaaag aacctgaaca gagatatttg cccacgccac gcagcgggcc
     2041 agaactgaat aacgaaatcg tggttgttta tgacctgttt tataacgtga cattgagata
     2101 atttcctgat gaacgaatta acgaacatag gcagacacaa ataatggcgt ctgcttgtca
     2161 gaaaaaccgg ggatttcccg gtttcctttt ccgcagaaaa ggaaatacct ttatgtaaga
     2221 aagtgcaccg ccacctgata tatccagtgt ctgtagccac tcatcaggtg gttgggcgcg
     2281 ggattatatt tttatttatg ggtaaaaatt tagctttttg tgcaagtcat taaatagata
     2341 cctaagttat atttcttact ctgcggacag tgaataattc attattcaga ctgaataata
     2401 atgacctgcc tttaattagc aactattgga aatacgtcga agtaggtata tacgccagga
     2461 taaaccgata tatttattat ggatattcat tttccttcat cggactgtaa attgcgggga
     2521 tatcgaactt acactactca tcgcaacact caaacagatt tatgaatttc gttcataacc
     2581 cctgacaaat cctgccgtct aatcgagctt ttccctttac gttatgcttc tcatgaacga
     2641 gaacacaact tattcatgaa ttaaccattc cggataaact atggaatcaa attcaggcat
     2701 cgaaaggaac tggctggttt ccgttaatag attaaatatc tattactctc ccgagagtaa
     2761 aactatcaaa tcaatgagta accgatacag cctcacaaat tctttcagat aattaacccg
     2821 tcactgcggc acgttctctc gtaaggcgtg cctgcagtgt aatccagtat gtatccggca
     2881 tagttgacgg tttaatagaa ctcaattcct ctccctggtc accaacaacg atatcttgta
     2941 taacacccca ggagatacga cattatgatg agatgcgata tgctcctcag caccaatcaa
     3001 cgccaggact gggccagtct tgagatcaga ctcattcccg ggactgactt cagtcgacat
     3061 atatcaatga cgttggtgca ataaaagata gccgatgtaa tacatctatt caaatttgtg
     3121 atacatgtca aaaaactggt tgaccaaact cgttatttat ataagggcac ttacgaagtg
     3181 cgctcttttt taaagcgagg aagtaccaat gaaagagaat aaagtacaac aaatcagtca
     3241 taaactaatt aatatcgttg tttttgtcgc aattgtagaa tacgcctatt tatttctcca
     3301 tttctattaa taacggcaat aaactgttca cttcagtgat atttaaaata tgcattctct
     3361 ccctttttgt aagtaattat tatatccgtg ggagaggaat acacgctgtc aggtaatcaa
     3421 tcatactgcg ataaatcatc ggccagtaaa gtggagataa cctccattgt cgaaaaatcc
     3481 atactctcag cgaaaccatc atcaatcact catccaggcg tttatgggag cgtcgccaac
     3541 ggctgttaac aatgccagac ttccccgttg cggaaattcc acataccact aatggtcaca
     3601 gggattgggt gttgaaatga tccggatgag catgtatctt tatggttatg ctataacata
     3661 acaggtaaaa atgatgaagc ccaatatcca tcctgagtat cgtactgtgg tgttccacga
     3721 caccagtgtt gatgagtact ttaaaatcgg ctcgactatc aaaacagacc gtgagattga
     3781 gctggatggc gtaacgtatc cgtacgtgac aattgatgtc tcttctaaat cgcacccgtt
     3841 ctatacaggg aagctgagaa cagtggcatc agaaggaaat gttgcacgat tcacccaacg
     3901 ttttggtcgt tttgttagca cgaaaaaggg gtcgtgatga aagttcttaa ctctctgcgt
     3961 accgcaaaag aacgccatcc agactgtcag attgtgaagc gaaaaggacg gctatatgtg
     4021 atttgtaaat ctaatccacg ttttaaggcc gttcagggtc gtaagaaaaa acgttgattc
     4081 aaaattcgac ggattaacga tatttgtctg attaataatc agatcggatt aatgttggtg
     4141 tgtttataac accaacatta attttcctgg ggatatattc ttcctgttca tttgaggcca
     4201 actgcctgac gtttctctac gaatattcca ttatcttaat gttgacttgt tgaccagctt
     4261 cgcccctgta tgctggcatc aactctcttt tagactgaac acgccactca gtctcctccc
     4321 tttgcggcgc agcctgcatt ttcactcaaa ctgttaagat gataaatgtg gtaaatctgt
     4381 tggtactaac ataaaaacgt ttacgccaca ggaacagtct gatccaccgg taaccccgtc
     4441 gccgacgttc gagtgccagt tagagtaacg cgcacagata actgcactca gtgccctgac
     4501 aaagaggcca tcgttcctgt gacagctggc agccttcgtt taacttcact taatctggct
     4561 cttgagggct taccgaacag atgacgtaca tacgcccgtt caattttcca tcacttattg
     4621 taatgaacac ctataaccat tttgtgcggc atgttaatcc attaaaattt cttactgatt
     4681 ggcaaatcat cttcaatgac agctcattat agttttatat tctatccctt acccttaaaa
     4741 cttgtttttt tactagtcca tcacacagcg cattaagact attcctaaca cttcagggca
     4801 aagttcctga ccaatataaa atgcaagtaa gaattgaacg ttatattgcc aataacctta
     4861 tgaaaccaaa tgtctttttc ttcttatcaa aaaaacaata ttttcagttt ttctaaatat
     4921 tgacttaacc attgaattcc ttttccgttc acatattgac actcatcaaa aaaaaaacat
     4981 aaatttaagc ccaatcgaaa ataattaaac ttaatctcgt ttaaccttta ttgatatgta
     5041 ctacgtatct tatttacttc cggtttacta aggaaactga atgcacctgt aaaaattaca
     5101 ggtttggaaa gtagtgacat ggcaaagtga ttacagtagg gactatgagg ttaaaaacca
     5161 tatggaatgt caaaaccgtt ctgataaata catctggtct ccccatgacg cctacttcta
     5221 taaaggacta tctgaactga ttgtggatat cgacagatta atttatctat cgttggagaa
     5281 aattagaaaa gatttcgtgt ttatcaatct caatacggat tctttatctg aatttataaa
     5341 ccgtgataat gaatggttat ccgcggtaaa ggggaaacag gtcgtattga ttgcggccag
     5401 aaagtcagaa gccttagcaa attattggta ctacaacagc aatattaggg gcgtggtata
     5461 cgctggactg agtcgtgata ttagaaaaga actggcctat gtgattaatt gcaggttcct
     5521 gagaaaagat attaagaaag ataaaatcac ggaccaggaa atggaaatta tccgcatgac
     5581 ggcccaggga atgcaaccta aatcgattgc cagaattgaa aattgtagtg tgaagacagt
     5641 gtatacccat cggcgtaatg ctgaggccaa gctgtactca aaaatatata agttggttca
     5701 gtaaactcca ggcaagttag ttttaaaaaa tgactcactg ggacatcacg tcctcaattc
     5761 aactcgggaa gaaatgcaat gaaaaaaagg ttctggcaat agctctggta acggtgttta
     5821 ccggcatggg tgtggcgcag gctgctgacg taacagctca ggctgtagcg acctggtcgg
     5881 caacagccaa aaaagacacc accagtaatc tggttgtgac gtcactcggt agcttggcgt
     5941 tccagtatgc cgaaggcatt aaaggtttta actcacagaa aggtctattt gacgtggcta
     6001 tcgagggtga ctcaacggct accgccttta aactgacctc acgtcttatc accaacacat
     6061 taactcagtt ggatacctca ggttccacac tgaatgtggg cgtggattat aacggcgcgg
     6121 cagtcgaaaa aactggcgat accgtgatga tcgataccgc caacggcgta ctgggcggca
     6181 accttagccc gctggctaac ggttacaatg ccagcaatcg taccaccgca caggatggtt
     6241 tcaccttcac catcatcagc ggcaccacca atggtaccac cgcagtaacc gattacagca
     6301 ctctaccgga aggcatctgg agcggcgacg ttagcgtaca gttcgacgcg acctggacca
     6361 gttaatctct ctgatgtacc agcaggggta gcccccctgc tttattcctg gacggaattg
     6421 ttatgaaaaa gcaccttctg cctctcgctc tgctgttttc cggaatatct ccggcccagg
     6481 cgctggatgt cggcgatata tcatcgttta tgaacagtga cagcagcacg ctgagcaaaa
     6541 cgatcaaaaa cagtaccgac agtggtcgcc ttatcaatat ccgtctcgaa cggctctctt
     6601 caccgcttga cgacgggcag gttatctcaa tggacaagcc ggatgagttg ctactcactc
     6661 ccgccagctt gctgctaccc gcccaagcca gcgaagtgat ccgcttcttc tataagggac
     6721 ccgcagatga aaaagagcgc tactaccgca ttgtctggtt tgatcaggcc ctcagtgatg
     6781 cgcagcgcga taatgccaac cgcagcgctg tggccactgc ttccgcccgc atcggcacca
     6841 ttctggtcgt cgcccctcgt caggcgaact accactttca gtacgccaac ggctccctga
     6901 caaatacagg aaatgcgacg ctgcggatcc tcgcctacgg cccctgcctg aaagccgcca
     6961 acggtaagga gtgtaaagag aattactacc tgatgccggg caagtcgcgt cgttttaccc
     7021 gcgtggacac tgcggataac aaaggacggg ttgcactttg gcagggtgat aagttcattc
     7081 ccgtgaaata gatagctgtg cagatggata acgacaatgc ctttacgacg gttctcccca
     7141 ggactgaaag cccagtttgc cttcggcatg gtctttttgt tcgttcagcc cgatgccagc
     7201 gctgctgaca taagtgcgca gcaaataggt ggggtgatta ttccgcaggc cttcagtcag
     7261 gcgcttcagg acggcatgag cgtcccgctc tatattcatc tcgccggtag tcagggtcgc
     7321 caggacgatc agcgaatcgg cagcgctttt atctggctgg atgatggaca gctacgcatc
     7381 cggaaaatac agctggaaga gagtgaagat aacgccagtg tcagcgaaca aactcgacag
     7441 cagctgatgg ctctggcgaa cgccccgttc aatgaggccc ttaccatccc ctgactgaca
     7501 acgcgcagtt ggatctcagc ttgcgccaac tgctgctgca gctggtggtc aagcgcgaag
     7561 cgctgggcac cgtactacgc tcacgtagcg aagacatcgg gcagtccagt gttaacaccc
     7621 tcagcagtaa tctgagctat aacttgggcg tctataacaa ccagttgcgt aacggcggga
     7681 gcaacacatc cagctatctg tcgctgaata acgttactgc gctgcgcgaa catcatgtgg
     7741 tgctcgacgg ctcgctgtac gggatcggta gcggtcaaca ggacagtgaa ttatataaag
     7801 cgatgtatga acgcgatttt gccggtcacc gatttgccgg tggaatgctc gacacctgga
     7861 acttgcagtc attagggccg atgaccgcca tttcagcagg gaagatttac ggcctttcct
     7921 ggggaaacca ggccagctcc accatcttcg acagcagcca gtcagccacg ccagtgatcg
     7981 cctttttacc ggcggcgggc gaagtacatc tcacccgtga tgggcggcta ctaagcgttc
     8041 agaacttcac tatgggcaat catgaagtgg atacccgggg tctaccgtac ggtatttacg
     8101 atgtggaagt tgaggtgatc gttaacggtc gcgtgatcag caaacgcact caacgggtca
     8161 ataagctgtt tagccggggc gcggcgtcgg tgcaccactg gcgtggcagg tatggggcgg
     8221 tagctttcat atggatcgct ggtcggaaaa cgggaaaaag acgcgaccag ctaaagagag
     8281 ttggctagca ggtgcctcga cctccggctc attgagtacg cttagctggg cggcaacggg
     8341 atatggatac gataatcagg cggtgggtga aacccgtctg acgctgccgc ttgggggggc
     8401 gatcaacgtt aacctgcaaa atatgctggc cagtgacagc tcatggagca gcatcggcag
     8461 catcagcgct actctaccgg gaggctttag ttcgctgtgg gttaaccagg aaaaaacccg
     8521 tattggcaat caattgcgac gtagcgatgc cgacaaccgt gcaatcggcg gcacactcaa
     8581 cctgaactca ctgtggtcga agctgggcac attcagcatc agctacaatg atgaccgccg
     8641 ttacaacagc cattattaca cggcagatta ctatcaaaat gtctacagcg gtacctttgg
     8701 ttcgcttggc ctgcgggccg gtattcagcg ctataacaac ggcgacagca acgccaatac
     8761 agggaaatat atcgctctcg atctctcgct accactgggc aactggttta gcgcagggat
     8821 gacccatcaa aacggctaca ccatggcaaa cctgtcagca cgcaaacagt ttgatgaagg
     8881 gaccattcgc actgttggtg ccaatctgtc acgtgccatc tccggcgata ccggtgatga
     8941 caaaaccctc agcggtgggg cgtatgcaca gttcgacgct cgttacgcca gcggaacgct
     9001 gaacgtcaat agcgcggcgg acggctacgt caataccaac ttgaccgcca atggcagcgt
     9061 cggctggcag ggtaaaaaca ttgctgccag cgggcggact aatggcaacg ctggggtgat
     9121 attcaacacc gggctggagg acgacggtca gatcagcgcc aaaatcaacg ggcggatttt
     9181 cccgcttaac ggcaagcgta actatctccc gctctctccc tatggaagat atgaggtgga
     9241 gttacagaac agcaaaaact cactcgacag ttacgatatc gtcagcggcc gcaaaagtcg
     9301 tctgactctc tatccaggca atgtcgctgt cattgagcca gaggtgaagc agatggttac
     9361 cgtctccggt cgtatccgtg cggaagacgg cacactgctg gctaacgcac ggattaacaa
     9421 ccatatcggc cgaacccgaa ccgatgaaaa cggcgagttt gtcatggacg tggataagaa
     9481 ataccccact atcgattttc gctacagtgg caataaaacc tgcgaagtgg ctctggaact
     9541 caaccaggcg cgcggtgccg tctgggtcgg tgatgtggtc tgcagcggcc tctcatcgtg
     9601 ggcggcggtg acgcagacag gagaagagaa tgagagttaa cctactaata a
//
LOCUS       DAJTNN010000158         9613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTNN010000158 DAJTNN010000000
VERSION     DAJTNN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.158"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(95..1459)
                     /gene="tdcG"
                     /locus_tag="OO150_003336"
     CDS             complement(95..1459)
                     /gene="tdcG"
                     /locus_tag="OO150_003336"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5517371.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGALSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1531..1920)
                     /locus_tag="OO150_003337"
     CDS             complement(1531..1920)
                     /locus_tag="OO150_003337"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5517372.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1934..4228)
                     /gene="tdcE"
                     /locus_tag="OO150_003338"
     CDS             complement(1934..4228)
                     /gene="tdcE"
                     /locus_tag="OO150_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5517373.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFEAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4262..5470)
                     /gene="tdcD"
                     /locus_tag="OO150_003339"
     CDS             complement(4262..5470)
                     /gene="tdcD"
                     /locus_tag="OO150_003339"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5517374.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5496..6827)
                     /gene="tdcC"
                     /locus_tag="OO150_003340"
     CDS             complement(5496..6827)
                     /gene="tdcC"
                     /locus_tag="OO150_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5517375.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6849..7838)
                     /gene="tdcB"
                     /locus_tag="OO150_003341"
     CDS             complement(6849..7838)
                     /gene="tdcB"
                     /locus_tag="OO150_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5517376.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7937..8875)
                     /gene="tdcA"
                     /locus_tag="OO150_003342"
     CDS             complement(7937..8875)
                     /gene="tdcA"
                     /locus_tag="OO150_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5517377.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            9064..9408
                     /gene="tdcR"
                     /locus_tag="OO150_003343"
     CDS             9064..9408
                     /gene="tdcR"
                     /locus_tag="OO150_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5517378.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         2561 a         2449 c         2198 g         2405 t
ORIGIN      
        1 aattaatcgc aagagaatat tcattctcta tttgcggcag atcacaaaaa aaggtgcaca
       61 tttgtgcacc caaggatgaa agctgacagc aatgtcagcc gcagaccact ttaatggcca
      121 gtcctccgcg tgatgtttcg cggtatttat cgttcatatc tttgccggtt tcatacatcg
      181 tctcgatcac tttatcgagt gaaacacgcg gtgccgaggt gcggcgcatc gccatccgcg
      241 cggcgtttac tgctttcacg gcattaatgg cattacgttc aatgcacggg atttgtacct
      301 gtccggcaac cggatcgcag gtcagcccaa ggttatgctc catcgcgatt tccgccgcat
      361 tgcatacctg cgccggactg ccgcccagta gttcagttaa ccctgccgcc gccattgaac
      421 aggccacgcc aatctccccc tgacagccga cctccgcgcc agagatggag gcgttcattt
      481 tatacagcgc gccaatagcc cccgctgcca gaaaatagcg ggcaattgac cgctcgttta
      541 ccggacgacg gaacttatcg taataagcca gcactgccgg aataatgccg cacgcaccgt
      601 tagtcggtgc cgttaccacg cgcccgccag ctgcgttttc ttcactaacc gccagcgcgt
      661 acatgttgat ccagtcgatg acattcatcg gatcgttaga gatgttatcg ctagaaacca
      721 gctgacgacg cagcgctacg gcacgacgcg gcacattgag cggaccaggc agcacgcctt
      781 cagtgttcat cccgcgttca ataccgtcat gcatcacttg ccagatacgg gcaaaccctg
      841 cgtcaatttc cgctttgctg cgcagcgcca gctcgttgtg catcatcagg ccagatatag
      901 acaggccgtt gtaatcacac attttcagca gttcacctgc tgagtggaaa tcgtaaggta
      961 cggacgtttc gacatcgtgc gacaggccga agtgttcttc ttcgacaata aacccgccgc
     1021 cgacagagta ataggtttta cttaatagcg cttcctgtcc cttccaggca gtgatccgca
     1081 ttccgttctc atggcgaggc aacatttcgg gatggaagat aatgttcttt gctacaggaa
     1141 aatcaacaat atgcgcacca gatgccactg gcagccgccc gctgcgcgtt actaactcta
     1201 taaatgcagg gatctcatca atgacaacat cctgcggact gtttcctgcc agtcccatga
     1261 taatggcgac atccgtggca tggcctttgc ccgtcagtga caacgcccca tacagatcga
     1321 ccacaatatg gctcgtcgcg gttaataagc cgctactttc cagccgatca ataaaacttt
     1381 ttccggcatt cattggcccc acggtatgcg aactggaggg accaatccca attttgaaaa
     1441 tatcgaatgc actaatcata tccacaccct cggattgccg ttcagtgaag tggagcggaa
     1501 cgaccttgcg accgtcccgc tcacgaggct ttacgcacta cgtactgcga tagcttcaat
     1561 ttccagcttc acatctttcg gcaaacgcgc aacctgcaca cagctccggg tcgggtaggt
     1621 cgcctgatgc tcatcgaaga actgcttata gacttcattg atggtggcaa aatcattcag
     1681 atcggtgatg aacacggtca tcttgatgat atcgcccacg ctcagcccgg cagcaaccac
     1741 gatcgctttg acgttttcga ggcttaaacg cgcctgatct tgcacatcag ccgggatctc
     1801 accggtctgc gggcaaaccg gaatttgccc ggaggtgaag accatgctgc ctaaatcaac
     1861 gccctgaaca taagggccga ttgcgcctgg ggcacgttgc gtttcgataa tctttttcat
     1921 acatcctccg gcgtcagagc gcctgggtaa aagtacgtga aataacatcc tgttgctgtt
     1981 cacgggtcag tgcgttgaag cgcacggcgt agccagagac acggattgtc aggttaggat
     2041 atttttccgg gtgctcgatg gcatccagca gcatttcccg attcattatg ttgacgttga
     2101 ggtgttgacc gccttcgaca tccgcttcgt ggtggaaata cccatccagc aggccgacaa
     2161 ggttagtttt acgtactgga tcttctttgc ccagcgccgc aggaacgatt gagaaggtgt
     2221 acgagatccc atctttggcg taggtgaacg gcagtttcgc caccgacgtc aatgaggcca
     2281 cggcaccttt gcggtcacga ccatgcatcg ggttagcgcc cggcgcgaac ggtgttccgg
     2341 cgcgacgacc gtccggcgta ttaccggttt tctggccgta caccacgtta gaagtgatag
     2401 tcagaatcga ctgggtaggg acggcgttgc gataggttgg cagcgcttta attttcttca
     2461 taaagcgttc aaccaggtcg caggcaatgc tgtctacgcg ctcgtcgttg ttgccgtact
     2521 gcggatattc accgtcgatt tcaaagtcca ccgccaggcc gttttcgtca cggattggtt
     2581 tcacgcgggc atatttgatg gcagacaggg agtccgtcgc caccgacagg cccgcgatgc
     2641 cgcatgccat agtgcgatag acatcacgat cgtgcagcgc catcagcgaa gcttcgtagc
     2701 tgtacttgtc gtgcatgtag tgaatgatat tcagcgcgct gatgtactgc accgccagcc
     2761 agtccatgaa gtgatcgagg ctgtccatca ctttgtcgta atccagcacg tcgtccatca
     2821 gcggtgctgt tttcggcccg acctgaatct tcagcttctc gtccaccccg ccgttaattg
     2881 cgtagagcag cgttttcgcc aggttagcgc gtgcaccaaa gaactgcatt tgcttaccaa
     2941 tcaccattgg gctgacgcag caggcaatcg cgtaatcgtc gctgttgaag tcagtacgca
     3001 tcagatcgtc attttcatac tgcaaggaag aggtgacgat cgacacctgc gcggcatatt
     3061 ttttgaaggc aatcggtaat tcttccgacc aaagaatggt caggttaggt tccggtgccg
     3121 gccccatagt gtgcagggtg tgcaaatagc ggaaggagtt tttggtcacc agcgtacgac
     3181 cgtccagccc catcccgccg atcacttccg tcgcccagat tgggtcgccg gagaacagcg
     3241 aatcaaattc cggtgtacgc aggaagcgta ccatacggat cttcatgatg aagtgatcga
     3301 tcagttcctg tgcctgctgc tcattgagta cgccagcttc aaagtcgcgc tcaatgtaga
     3361 tatcgaggaa cgatgccgtg cggcccagcg acatcgcgcc gccattttgc gatttcactg
     3421 ccgccagata agcgaagtag agccactgca ccgcttcttg cgcattctgc gccgggcgag
     3481 agatatcaaa gccatatttc gccgccattt cctgaatctg caacagcgca tgacgatgct
     3541 ctgccagctc ctcacgcaga cggatggtgg cttccagatc ctcgcctttt tccagacgag
     3601 actggagatc ggcaaattgc agttcgcgtt cacgtaccag ataactgatg ccatacagcg
     3661 ctacgcggcg atagtcaccg ataatgcgcc cacggccata gccatctggt aaaccggtca
     3721 gcacgccaga tttacggcag cgcagcatat ccggtgagta aacatcaaat acgccctggt
     3781 tatgggtttt acgcagatcg gtaaacagat attcaaattc actgtccatt tcacggccat
     3841 aggcgtggaa tgaactttta atcatattaa tgccaccgaa cgggtgtagc gcacgtttca
     3901 acggcgcatc cgtttgcagg ccaacaattt tttccagcgg ctggttaata tatcccgcat
     3961 catgagcggt aattgtggtg gcaatattgg tatcgaaatc aaccggcgcg tgggttgcgt
     4021 tttcgatacg gataccttcc attacttttt cccacaattc cgtggtggca ggcgtcgctt
     4081 cggcgaggaa agattcatcg ccttcatacg gtgtatagtt atgttgaata aaatcgcgga
     4141 cattaatttc gtttttccag tccgtacctt taaagccaag ccatgcgtcg gcgtacagct
     4201 tatcgctggt atcaatatct accttcatga aaaataatct ctctacaata cttcaactaa
     4261 attatgcaaa ttctgcgggc gcgttaactt tgcctaaatg aatggcatcc aaagcaatca
     4321 ttttttcttc gttagtcgga ataacagcgc aaatgacacg cgcattttca ctggaaacaa
     4381 ttcgctcacc acaggagtta gagcgattat tcatttctgt atcaatctct acgcctaata
     4441 cagccaaatg ttccatgacc agacgacgaa ttaagcttga attctctcct attccgccgg
     4501 tgaatataat tccatccagg cgacgtaatg aagctgcgtg tccggcaata tgacgggcaa
     4561 ttcggtgaac aaaggtttta attgccagtt gcgcgcgttc gtgaccttca tgccaggctt
     4621 tttccagaac acgtaaatcc gaagaaagac cggaaatacc taataatccc gactctttat
     4681 tcactacgcg ttccaagtca cccaagctct ggttggtttg gctggcgacc caggacatcg
     4741 caccaaaatc gacatcgcca ctgcgggtac ccatcatcaa gccttccagc ggcgtcattc
     4801 ccattgaggt gtcaacactc tgaccgttgc gaactgcgca gattgacgcg ccattgccaa
     4861 gatgcgccac aaccaggccg gagtcatctt ccgctagatt cagcaaagaa tgtgcgcgct
     4921 gggaaacata gcggtgcgac gtgccgtgga aaccataacg gcgtacacct aactcttcat
     4981 aatatttcca cggcaggccg tataaataag cttccggagc catcgtctgg tggaaactgg
     5041 tatcaaatac cgccacctga gttacgcccg gaaataattg ctgcgccgat tcaataccac
     5101 ttaaattggc gtaattatgc aggggtgcca gtggagaaac gcgacggata ttatcaatga
     5161 cctcatcggt aataatggcg gactcggtaa aaatactgcc gccgtgagcg atgcggtggc
     5221 caattaaggc cacactgtca tttaaattcc gtttttccag ttcaaatgca attgccttca
     5281 atgcaccttc gtagctgtgg tgagccagcg gtgctggctc tcccccattt acggataaga
     5341 atgcattttc tgagttaata ccgtcggcaa tacctgacat taatacttca cagtcgctgg
     5401 catcgagtac ggaaaactta atcgaagacg aaccacagtt aataaccaaa acaaccggaa
     5461 attcattcat ctcttttctc atcctgagtt acggattaaa acagtttgta tacgatgttc
     5521 aggatggtca gcagaccaat cacggtaaca aacacgttat ccagacgacc acggtatttc
     5581 gccagagacg gtgctttacg gatggcatac atcggcaaca ggcacagcag ggatgcgata
     5641 atcggtgcgc ccatggcttc aataaggtcg aggatgttcg ggttggcgta ggcaacaacc
     5701 caggtggagc ccatgatgaa gatcatgctg atagtgttca gtttacccag cgacacttta
     5761 gttttgtcgc ctttataacc gaacttcaga atcagaccat tcaagccttc cagcgtcccc
     5821 agatagtgac cgaagaaaga tttgaagata gccacgagtg cgatgatgga agccgcatat
     5881 tccagtgtaa tcgcgaacgt tgttttggta ccggtcatgg acgcaaagtg gttagccaga
     5941 taagaaagca ctggaatatt ctgcgctttg gcttccgcca tgttggccgg agacagcgta
     6001 aacaggcagc taaaggcaaa gaacatcacc actgcaacca tcagcatgct ggcacgagaa
     6061 atgatttggg aacatttacg ttcggtgaag tcgcgaccga agtctttctc atactcttca
     6121 cgtttagaaa ccacgaagga agagacgatt ggcgagaagt taaaggagaa aaccatgatg
     6181 gaaatcccca gccagacagt gatcaggata ccgtcatgac cggttaacga cagcgaaccg
     6241 aggtcaacct ggtcgataac tgcagagttc cagtaaggga tcagcgacag agaaatcagc
     6301 accaggctgg cgataaacgg ccataccagg tagctcatca ctttaaccat cagatcctta
     6361 ccaaaccaga tgacgaaagc catcagcagc aacaggaaca gcgccacaaa gccgcgattc
     6421 agcggtgcaa atccgagctg gttttcccag aacgtcataa aggtattggt aatagtaacg
     6481 ccataaatcc acagcagtgg gcaaatcgcg aagaagtaca ggaacgtgat aaccacgccg
     6541 ccagttttac caaaatgctc ttccaccgtt tccgtaatgt tgccggaagg gttagagcca
     6601 gaaagacaca gacgcgccag cgcccggtgg caataaaatg cgatggggta tgccaatacc
     6661 aacatcagaa gaatcgggat cagtccgcca aaacctgcgc ggatagggaa gaacagcacc
     6721 ccggcgccga ttgccgtacc aaacaagcct aacgtccatg tggtatctga tttacgccag
     6781 gacgattgtt ttgtctggct ggatacaatg ctatctgaag tactcatatc ctatcctcaa
     6841 cgaattaatt aagcgtcaac gaaaccggtg atttgagaga cgcgagaaag atcgatattg
     6901 ccgccggaaa taatactgac ggtttttctg ttttgaatat attggtctaa tttaccgctt
     6961 aataatgcag cacatgccag agcacctgcg ccttcggtga cgactttatt gcgctgaatt
     7021 aaggcaatca tactgtttct gatttcgtct tcgctgacca gcacgatgtc atcgactaat
     7081 tcacgaacga tttcgtaagt taaattaccc gggcgggaga catcacaacc atccgccagg
     7141 gtgccggtag ttcggtgcgt ggttatttct ccggagtgga aagaagccgc catgccgtga
     7201 acgttttctg actgtacgcc aataacacga atggtcgggt taatagattt aattgccacc
     7261 gcaataccag caattaaacc accaccacca attggcacaa tcacgttatc gacatcatag
     7321 agatcttcca taatttccag accaatcgtt ccctggccag caatcacttt cggatcatcg
     7381 taaggtggga taaaaatacg accttccatt tcgacaattt cgctcacttt agcgatagtg
     7441 tcgttgaagt tatcaccatg cagaacgact tctgcggagt agtcgcacgt tgccgctact
     7501 ttggattttg gcgcaccttt tggcatcacc actttaccgt cgatacccag catcgcgcag
     7561 gagagcgaaa ccccttgcgc atggttgccc gcagaacagg ccaccacgcc tttgcgtttt
     7621 tccgcatcgg tcagtgaact taatttatta aatgcgccac gaattttaaa tgaacccgta
     7681 cgctgcatat tttcaaactt caggaatatt tcacctttgc aacgttcact aaaatagttg
     7741 gagcgaggca tgcctgtttt ataaattcgc ccagccagtc gttgtttcgc ttcaataata
     7801 tcatcaatag caaccggcag atcgtatgta atatgcatta taaaacctct tcgcctgaat
     7861 taaatatgta gataaccgta accgacacct gcaaaacaga caggtggatt atttatgttt
     7921 aaataaaaac aaataactaa ccaacttcta ttaatagcct tcgtctacac ccattataag
     7981 atgaatactc tttggctaat tccaccaaaa ccgatgctgc ttttttaata cgataatttt
     8041 tcgaccatac cgcggcatat tgtgccacag gtaatgtttc ttcaactgga atagtaataa
     8101 attgatttga accaaaaggt gacgtcatat cacaaggaat tacagttaag aaatcagcat
     8161 tgagaacaag attataaatt gtcacgactg agtcggtttt aacgatgttt tcaatactga
     8221 tgccatttct ttgtaacgta gtaagaagct cgctgtagta ccccatattg gtttgtggca
     8281 acacccactg ttcgttcttc aacgactcca gcgtggtggt gccggtgcat gttcgggact
     8341 tactggctac cagcacaaac tcggactcga acagcggctc aacatgtaaa tcctgaagct
     8401 tcatttctgc acttaacgta ccaatcgcaa aatccagtcg accgtcgcgg attgccggta
     8461 agaacgaaga cagttgcgct tcatacatag aaacctgcgc tttcgggaac acctctttga
     8521 acttgttgat catccctgac ataaaagtaa aaccaatcaa tgaaggaaaa ccaaatgaga
     8581 cttccaccac cgcctcagaa gacataccgc ttatctcatt aaccatattt ttcatttcac
     8641 gggtaatgga ttcggaacgg gagagtaaca attgaccggc aggtgttaat gttacaccgg
     8701 tatttttccg caccactaat tccacaccaa aataatcttc aatatcgtta atgattttac
     8761 tgacggccgg ttgagttaac cctaattctt ttgcagccga gccgatagag ccacttctaa
     8821 tgacttcctg aaagactacc aggtgctgcg ttttcggaag aagaatagtg ctcataacga
     8881 cctacgttaa ttacctcatt gacggcatga agtgtatcaa aatgaaatga acaggatatg
     8941 tgcgaccact cacaaattaa ctttcattac tttccagggt atcgttatta caattaaata
     9001 tctttaaata tcaacaagtt aaaacataaa aatcagcata aaccctgatt tttatcaaaa
     9061 aaaatgacgg ggataaccat attttatggc gataacatca ttcgttatgt ggttaataca
     9121 aaaaaggggc tgagaccata tttcaagcaa ttaccggata attaccaggc aaaatttgag
     9181 ttaaatttaa tgagcaagtt ctcaaatttt attataaata aaccattttc agcgataaat
     9241 actgcggcac gtcacatttt ttcacgctat ttgttggaga acaaacattt attttatcaa
     9301 tattttaaaa tttcgaatac aggtattgat catctcgaac aattgattaa cgtcaacttt
     9361 ttctcttctg acaggacgtc attttgtgaa tgcaatcgtt ttccataaat tcttctcccc
     9421 tcataggcga cgaatagcat tttgtgttga ggatcacaaa acaaataatt gctggtcgcc
     9481 gcgataaggt cagacgaagt caacaagggg aattttcact gtgctttaca tcagcgtagg
     9541 acacagaatg ttgcttcgtt tactgtaacg ccgggtaaat gagcgttttt tgatagtgcg
     9601 taagtgtcgt gcg
//
LOCUS       DAJTNN010000159         9600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTNN010000159 DAJTNN010000000
VERSION     DAJTNN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.159"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(174..515)
                     /locus_tag="OO150_003344"
     CDS             complement(174..515)
                     /locus_tag="OO150_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5517379.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(518..4288)
                     /gene="tamB"
                     /locus_tag="OO150_003345"
     CDS             complement(518..4288)
                     /gene="tamB"
                     /locus_tag="OO150_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5517380.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAQEEVVEPKIENPQPDEKPLGETLKDLFSRPVL
                     PEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDSNQG
                     IVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGPVDM
                     DLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMRTAV
                     KGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTLNGI
                     NTAKEFPEWPSKLNGLIKTSGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGNSYM
                     QWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKVRGT
                     VEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNINLV
                     TLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPWSLT
                     RDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLKPFM
                     PETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTLNLT
                     AELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIFTRG
                     EKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMRSTL
                     AGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFEATP
                     NLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNLIVH
                     VGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGELLF
                     SGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSYLLR
                     GQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQVVVS
                     GYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(4285..6018)
                     /gene="tamA"
                     /locus_tag="OO150_003346"
     CDS             complement(4285..6018)
                     /gene="tamA"
                     /locus_tag="OO150_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5517381.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRTQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSCGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6224..6862
                     /gene="msrA"
                     /locus_tag="OO150_003347"
     CDS             6224..6862
                     /gene="msrA"
                     /locus_tag="OO150_003347"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5517382.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNEPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            7185..8528
                     /locus_tag="OO150_003348"
     CDS             7185..8528
                     /locus_tag="OO150_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5517383.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(8590..8796)
                     /locus_tag="OO150_003349"
     CDS             complement(8590..8796)
                     /locus_tag="OO150_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5517384.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            9121..>9600
                     /locus_tag="OO150_003350"
     CDS             9121..>9600
                     /locus_tag="OO150_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5517385.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     "
BASE COUNT         2204 a         2638 c         2364 g         2394 t
ORIGIN      
        1 agcgtgcccc cgttttaccc ctgcacattt gtgaacgcca gcagagtttt ttatgcgatt
       61 gcagatatgg aaagcggctc ggagagaaaa gcaaaagatg gaaaagttac aaaaaaaaat
      121 cgcgagatac atcgcataaa acaacgccac ccgaaggtgg cgtgctttta ttattactta
      181 tccctgtcta accagtcgcc gctttcaatt agctttaatc cctcgacggg tcgttgataa
      241 acgtacatcc atgcactccc atacggcgtc tgaatcaact ggcgcgcgta ttcaccgccc
      301 ctggtgcgca aggcatcaag ttcggccagc gtggcgttgt caatacgata aacttcaccg
      361 tgtaccgttc cgttccccgg aactgcgcct ggatagtggc ccaggctata caactggtag
      421 ttatcgatac tgaaatcgcc cagtaactgg gcattggtca tccagtgact gttgccttgt
      481 ttgtggcgta aactgccgta gacaaatatt cgcattgcta aaactcgaac tgatagagca
      541 aatccagtgc ctggtctaca ccagacacgg cttccagata tagcttaggc atcaggcgat
      601 aacgtaacgt gagtgttgct atagagtcaa atatacccac gccgtatttc acttgcaggc
      661 ctggcaatac atagccgctg accactacct gggaggagtc gcctactccc tgggtgtcga
      721 gcgctaaatt gcttacgcca aacgtctcgc cgattttacc cacaatctgg ccactttgcg
      781 caacccccag accaatcagc atcgaggtca ttgccgcact gtcgctctga tcgctctcca
      841 gcccttgtcc acgtagcaaa taagacaatg cagcttgttg cgacatcgcc gggtcagaga
      901 agatctccgc tttcggttcg tccgccagac cagtgacgcg aactccggcg attacgtcgt
      961 cttctgtagc atccgggtta cgaatagctt caatattaag atacggttga tctggcggac
     1021 cagagaacag tagctcaccc ttacgcacaa tcagatcctg accataggca tggaagcgcc
     1081 cttcagggat gttgatctgc ccgttcaggc ccagcccttg tttgtcctga acgacgttaa
     1141 gatcgcccgt cagccgcgct ttcaggccaa aggcgtcaat gcgcacattg ttgccaacgt
     1201 ggacaatcag gttactgtta atcggaatcg acgccgtttt cggctcttcc ggttgcaggt
     1261 tatcgttaag catcaccaca tcgctggaga cgcctactgc gctttccggc agatcgtgca
     1321 ccacgatgcg cgcccacggg acatccacgc gaccatcgag ggtaaacagg tttggtgtag
     1381 cctcgaatac aacatctggc gatacatcca ttcgtaccat cggcggcacg gtgatccgca
     1441 ctttactgcc tttcgccgtt actcgtgccc gccagttttc aatttggctc cagtcggcat
     1501 caccgttcag gtagatttca ccctgctggg tccgtactgt accggcaagc gtcgagcgca
     1561 taccgttaaa gttgaccgca agctggctcg gctgcatatc aaacggcata aagttgccgt
     1621 cgatatccac acccgtaacc tgaagctgac caaacaactg cgggctttgc acatcaccac
     1681 ccagacgcaa gttggcactc accatccccg ctgctttttc cccacgagta aagatggggt
     1741 ttatcatcgc aaggttgaag ttacggatat tgacgttgcc accaagatta cggcggcctt
     1801 gcggatcggt caccagcacc tgtccatcaa actggccgtt attggtcagg cggatggtcc
     1861 agcccaattc ggcacggttg ttacgcaatt ccgccgtcag attcagtgtc tgaaacgcca
     1921 ccggcagcgc cgcatcgttg acggtttgcg ttacctgcac gttacgcccc gaaagggtga
     1981 tactgccctg cggcagcccc tctttcgtgg tgtcccaggc aacatccgct ttacccgtga
     2041 agataccgct ggcctgagtg gtttctggca taaacggttt cagcatggcg aagtcgaagc
     2101 ggttgagatt caccaccgca cgcccttcgg cccccgcatc gatagtttgc ggcacgcaca
     2161 gttccgcatt cgggttaagc cagcagtgtg gcccgatgct gattttttgc tccttattgc
     2221 ggtaatccag cgcaatatcg cgggtcagcg accacgggcc gaccagcgtc tggaagcggg
     2281 tattgctaag agttcccttc cagcgttctt ctttgcgatc aaaacttcct gccagattaa
     2341 gctgcccgga gacaggctcg ccctgaatcc gcaactgtag ctcgtgctgc ttttcgctgc
     2401 ctttggcatt cagggtgacg aggttgatat ttacatccgg ctgcgaaatt tgctcaacgc
     2461 gtacgtcgag tttcccggcg atctgatcgg tggatttaat gtcgccttcc acgcgaacct
     2521 gcgtcacgga aagttcctgc cagcgcaggc cgcgcgcggt gatatctgcc agtagttgtg
     2581 gcgcttccac cgtgccgcgt actttcacca gccctttcgc tgtaccgcca agccccggca
     2641 gcgcgttatc cagccccggc gcgttgatgg tggcatcaag attgagatct tttaccccca
     2701 gctcgccttt cacttcggca ctgtttggcc ccagttccag atgaagccca gggatcatcc
     2761 actgcatata actgttgcct ttcagcgtgc cgtcaacgtt cactttgttc tgtttgacgt
     2821 taccggtcag cttcagttct ggcacctcca tctgccaggt gccgccgtac aggctaccgc
     2881 tggttttaat caagccattg agtttcgacg gccactccgg gaactctttg gcggtgttaa
     2941 tgccattaag cgttagctca ccgcgccaac taatggcctg ctgccagtcg agcaacgcct
     3001 tgagttcagt tttcccttcc agcgccgcga cggtgagttt gtcgagattg acctgctgtt
     3061 cattaccttt ggcatcaagg gtaatggtgg ctggcgggat ctcctgtccc ttcactgccg
     3121 tacgcataga gagcgtgtaa tcggtcattt tgccggtaag tttcagtttc agatcatccg
     3181 cctgatactg cttctcacca gtgaacggcc agtaaagctg tttgctgttc acttccacgt
     3241 tgagcggcaa tccggcttcc gccagtcgcg tctgggcgcg taaatccata tccaccggac
     3301 cggaaagatt aacgccaatc tccagctgtt cgcgcagcgc gccgcccact tttagcttca
     3361 ctttttcacc tttcaacggc tccacgttca gtgtgctgtt gagggtgata tccaccggcc
     3421 agttgtctga cagctgcgcc gtgccgctgg cgttgacgat cccctggttg gaatcgatat
     3481 ccagggcgtc cagtttagta ttgccgtcaa tgctgctcac tttcagcagc atggtgcgca
     3541 cggtgatgtc cgtgtcgccc gtcacgcgta actgctcgcc tttaaactct tcaatgttca
     3601 ggttaagcgg caaatgcaag tcggtcattt ccggcaatac cgggcgagaa aagagatctt
     3661 tcagcgtttc gccgagcggc ttttcatccg gctgcggatt ttcaattttc ggttcgacca
     3721 cttcttcctg cgccactttc ggcagagcaa tcagcaggcc tttcagcgac gtcggtttca
     3781 gggtcagggt tttctcctgc cagttcaggc cggaggtgaa gtccatcacc gacaccgtgg
     3841 tgtcatcaat cttgatgttg acgttgtcca gcgccacccg tgtcagggtg atgggatacg
     3901 gcgtggagag atccagcgga ccgctatctt cctcttcttc aacctgttca gaaggaggca
     3961 tttttttact gtcgatgttg acctgaatgt ctttcagcgc caggtcatta atacaaacgc
     4021 tactgttcca caggcactca agaccgaccg ccagatgcag attacccgct tttaccgcca
     4081 cgcctggctg ctcataacga acgtcagaca aggtgagatc gcgccagccg ccggtgacct
     4141 tgccaatatc cagtcctggc acccagcgat ccgccgcttt aaatacaaga tgcaggccgc
     4201 tggtggtgcc caccagaaac gccaccgatc ccagcaacag taagataacg ataaccacgc
     4261 cgaggctgat ttttttccat aaactcataa ttctggcccc agaccgatgt aaaactgtaa
     4321 cccgtgttcg tctttatccg cgaccggtac ggcaaaatcg agtttgattg gcccgaccgg
     4381 cgattcccag cgcacgccga ccccggtacc ggttttaaag tcgctgcggc gaatatcgct
     4441 taccgcttcg ccactatcga caaacaccgc gccccaccat tttccggtca cgttgtactg
     4501 gtattccagc gatccggtta tcaacttcga ggcccctttc aggtcaccgt tggcgtattt
     4561 cggggcgata gatttgtatt tgtagccgcg aatactgcga tcgcccccgg cgaaaaaacg
     4621 cagatccggc ggtactttgt cgaaatcacc ggtttcaatc cagcccagcg tgccgcgtgt
     4681 aacgaaacga tggcgatcgt acagtgtgcg gatccagacg ttctgcgcct ggaaaacgga
     4741 gaaatcgaca tctgagcccc aggccgtgtt ggagtagtcg atagagtagc gttgcgagtc
     4801 gccccaggtt ggcatcaggc caccacaaga acgcgtgcgg ctaatcatca ccccaggata
     4861 aaacagcatc gtggtgttgg taatttcacc ctgagtaaag tggtcgagac tccagcgcag
     4921 gttaatggca cgctgccaac cgctggagag atcccagtag cgagaagcca ccagcgtagt
     4981 ggagtcagat tcggtatcgt tcaggtcagt gcgcttaaaa ccgccctgca ccaaataata
     5041 ttgttccagt ggattcttca gcagcggcat tttatagctg aagtcgaggg tctgttccgg
     5101 cgcggaaata ctggtactgg tggtcaaact gtgaccgtat gagttcatcc acggcttttt
     5161 ccacgtcgct ttcacgcgcg gtcccacgtc cgtagagtaa ccgaccccgg tttcgatggt
     5221 gttttctgtt cgcggcgaaa ccacgcccgt caatggtaat actttcgttt cgcgcgcttt
     5281 atcaaattgt ggagccacca ccaccgagtt aaaccagccg gtagcagaaa gtcggcggtt
     5341 cagttccgcc agatctttcg attcatactc atcgccctct ttaaacggca ccagattttg
     5401 caggtattca tcgcggattt gtgatccttc aaaggtcaca tgcccaaagc ggtaacgttc
     5461 gccactgtta taatcaatat cccagaaggc tttatgcagg ccgagcgcaa tgcccagctg
     5521 cgctttggta aattcgctat cgaaataacc tttacgcaac gcaatactgg ttaaggactt
     5581 tttgaaattt tcataatcgc cctggttcag taccgtacca atagccgggc gagtatcgag
     5641 caatttcaaa tagtctttat cggtccgcgc gccgccgcgc aataccacat cggtgccgcc
     5701 aattaacacc ggcacacctg gcgtgacttt ggcgatcaat acctgccgcc ctttctttgg
     5761 cggtggacgg agatcaaatt caatggtcgg ctggtaataa cccagcgctt tcagaccttc
     5821 acggatggca tcatcgacgc gtgcgcgaaa gcggcggtct ggcgtcactt catcactttc
     5881 aatcgtagaa agctgcgtac gaacgttctt ttccagctgt cccgataacc cctcgacctg
     5941 tagacggacg ttcgcggcga cggcagatcc gcttaagcag agtaagctta cacagcataa
     6001 ctgtcggata tagcgcacat tttctcctga atatcctttt ttcctgcccc tggaacgccg
     6061 ttaaacggct taacaaaaat ccagtaatat ggattaaaaa gcagactaaa ccccaaatat
     6121 ttcttatgtt ttactttaga cctattcacg gtggttattg tgtgcaaata cgcctcttgt
     6181 tacaacctta accccaatga ccgattttcg ggagagcgac accatgagtt tatttgataa
     6241 aaagcatctg gtttcccccg ccgatgccct gcctggacgt aacaccccga tgcccgtagc
     6301 cacgctgcat gcggtcaacg gtcactcaat gaccaatgaa cctgacggaa tggagattgc
     6361 catttttgcg atgggttgtt tctggggtgt ggagcgtctg ttctggcagt tacccggcgt
     6421 ttacagcacc gccgcaggct ataccggcgg ctatacgcca aatccgactt atcgggaagt
     6481 gtgctccggt gatacgggtc atgccgaagc ggtacgcatt gtttacgatc cttccgtcat
     6541 cagctatgag cagttgctac aggtattttg ggagaatcac gatcccgccc agggaatgcg
     6601 tcagggcaat gaccacggca cgcagtatcg ttcagcgatt tatccgctga ccccagaaca
     6661 ggatgccgca gctcgcgcca gtctggaacg ttttcaggcg gcgatgcttg ccgccgatga
     6721 cgatcgtcac atcaccacgg aaatcgctaa cgccacaccg ttttattatg ccgaagatga
     6781 ccaccagcaa tatctgcata aaaacccata tggttactgt ggaattggcg gaattggcgt
     6841 ctgtctgccg ccggaagcat agcgttacgg gtacaaatgt agtttgttga taaagtgctc
     6901 tttgtctatg cctgatgcgg cttaaacgac ttaccaggcc tacaaaatcg tccaaattca
     6961 atatattgca aggactgcgt aggcctgatc ggcatagcgc atcaggcagt attgcattta
     7021 tcagcagtct gaattttaac cctctggcga ctttacagta ccttacgcta tactagccac
     7081 tgaaaattcc ggttcacttt cttcgaatcg gctttcaatg tgtatttcac acaaattaat
     7141 caactttccc ttccgaggat ctggcctgaa aggtcggata agatatgtta aacagtattt
     7201 tagtcatact ctgcttgatc gctgtaagtg cgttcttctc gatgtccgag atctcgcttg
     7261 ccgcctcacg caaaatcaaa cttaaactgc tggctgatga aggcaatata aatgcccaac
     7321 gcgttctgaa tatgcaggaa aatcccggca tgttctttac cgtggtccaa atcggtctga
     7381 acgcagtggc gattctcggc ggtatcgtcg gtgatgcggc attttctcca gcttttcaca
     7441 gcctgttctc ccgctatatg tcggcagagc tctctgagca actgagcttt attctctctt
     7501 tctcgttagt gactggcatg tttatcctgt ttgcggattt aaccccgaaa cgcatcggta
     7561 tgattgcgcc agaagcggtg gctttgcgta tcatcaaccc gatgcgcttc tgcctgtacg
     7621 tttgcacccc gctggtgtgg ttcttcaacg gcctggcgaa catgatcttc cgtattttca
     7681 aactgccaat ggtacgtaaa gatgacatca cttctgatga catctacgcg gtagtggaag
     7741 ccggtgcgct ggcgggcgtg ttacgtaaac aggaacacga gctgattgaa aacgtctttg
     7801 agctggaatc ccgtaccgtt ccgtcttcaa tgacaccgcg tgaaaacgtg atttggtttg
     7861 atctccacga agatgagcaa agcctgaaga ataaggtggc ggaacatccg cactctaagt
     7921 tcctcgtctg taatgaagat attgaccaca tcatcggtta tgtcgattct aaagacctgc
     7981 tgaaccgcgt gctggctaac caaagcctgg cactgaacag cggcgtacaa attcgcaaca
     8041 cgctgattgt gccggatacg ttaacccttt cagaggcgtt ggaaagtttt aaaaccgcag
     8101 gtgaagactt cgcggtgatc atgaacgagt acgcgctggt ggtggggatc atcaccctca
     8161 acgacgtgat gaccacgctg atgggcgatc tggtcggtca ggggctggaa gagcagattg
     8221 tcgcccgtga tgagaactca tggctgattg acggcggcac cccaattgac gacgtcatgc
     8281 gcgtgctgga tattgacgag ttcccgcagt cgggcaacta cgaaaccatc ggcggcttta
     8341 tgatgtttat gctgcgtaag atcccgaaac gcaccgattc ggtgaaattc gccggctaca
     8401 aatttgaagt ggtggatatc gataactacc gcatcgacca gctgctggtg acccggatcg
     8461 acagcaaggc caccgccctt tcgccaaaac tgcctgacgc taaagataaa gaagaaagcg
     8521 tcgcgtaacc cccagaaaca tcaacggctc ctgaatcagg agccgtctta ttactgcata
     8581 gcactttggt taagccatct ctgtttgcag acgcataacc tgacggttaa cttcggacat
     8641 cactgacaaa tgcagcttat ccttcacttt tgggataaga atcttaccct tatcaaattc
     8701 aaaagcgcca acgtccttga tgtataaccg tccacggaac aggatcttta cgtacttcgc
     8761 cacctgaagt gggttgtacc gttggaaaat tttcattgtt atctcctgct gagtattacg
     8821 cctgtgcggt gccacaatcg gcccaactat tatgagacgc aaattttaat gcttagtgac
     8881 tatagactat ccgggcaatg tttccaccgc gtataacttt ttttacctaa aggttacaat
     8941 tattcagaat tatcttttta ccgaagcgcg gtcttcagta taagcattca tttttcatat
     9001 gatttgtgcg cttgaccgca aactggcatc acacttgcgg gaaattcgat aaatagcaca
     9061 tatgattaaa actcagatcc aagtggtcgg atcacctgca tatcataaga aggaaacacc
     9121 atgaccctac gcaagattct ggcactcacc tgcctgctgt tgccgatgat ggcttccgca
     9181 catcagttcg aaaccggtca gcgagtgccg ccgattggca ttaccgatcg gggcgagttg
     9241 gtgcttgata aagatcagtt tagctacaaa acctggaaca gcgcgcagtt agtgggaaaa
     9301 gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa aagagaaaaa cgcgacgctg
     9361 attgaagcga ttaaatcagc aaagttaccg cacgatcgtt accagaccac caccattgtt
     9421 aacaccgacg acgcaattcc gggttccggc atgtttgtgc gcagcagtct ggagagtaat
     9481 aaaaagcttt atccctggtc gcagtttatt gtcgatagca atggcgtcgc gcgcggtgcc
     9541 tggcagctgg atgaagaaag ttccgctgta gtggtgctgg ataaggatgg tcgcgtgcaa
//
LOCUS       DAJTNN010000160         9573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTNN010000160 DAJTNN010000000
VERSION     DAJTNN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.160"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..185)
                     /locus_tag="OO150_003351"
     CDS             complement(<1..185)
                     /locus_tag="OO150_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517386.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVS"
     gene            complement(221..1075)
                     /locus_tag="OO150_003352"
     CDS             complement(221..1075)
                     /locus_tag="OO150_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HCR5517387.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            1418..2248
                     /locus_tag="OO150_003353"
     CDS             1418..2248
                     /locus_tag="OO150_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5517388.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            2280..3215
                     /locus_tag="OO150_003354"
     CDS             2280..3215
                     /locus_tag="OO150_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5517389.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            3308..3550
                     /locus_tag="OO150_003355"
     CDS             3308..3550
                     /locus_tag="OO150_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5517390.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            3540..4991
                     /gene="panF"
                     /locus_tag="OO150_003356"
     CDS             3540..4991
                     /gene="panF"
                     /locus_tag="OO150_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5517391.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            5003..5884
                     /gene="prmA"
                     /locus_tag="OO150_003357"
     CDS             5003..5884
                     /gene="prmA"
                     /locus_tag="OO150_003357"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5517392.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            6213..7178
                     /gene="dusB"
                     /locus_tag="OO150_003358"
     CDS             6213..7178
                     /gene="dusB"
                     /locus_tag="OO150_003358"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5517393.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            7204..7500
                     /gene="fis"
                     /locus_tag="OO150_003359"
     CDS             7204..7500
                     /gene="fis"
                     /locus_tag="OO150_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5517394.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            7586..8470
                     /gene="yhdJ"
                     /locus_tag="OO150_003360"
     CDS             7586..8470
                     /gene="yhdJ"
                     /locus_tag="OO150_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5517395.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            8554..8733
                     /locus_tag="OO150_003361"
     CDS             8554..8733
                     /locus_tag="OO150_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5517396.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(8736..9398)
                     /gene="acrS"
                     /locus_tag="OO150_003362"
     CDS             complement(8736..9398)
                     /gene="acrS"
                     /locus_tag="OO150_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR5517397.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         2437 a         2282 c         2323 g         2531 t
ORIGIN      
        1 gagacactta aattgacatt gtcttttttt gcctcttctt tcatggcatt cgcaagatca
       61 atatagaaag gatgttgctg tgttaataat gacgcaccaa ttttatactc ttttgccaac
      121 gaattttgcg agaacaatgc caggctcacc aacatagctg aaccaagtaa tttaatattt
      181 ttcataaaca atcctcattg tttttgtagg gtacaacgtc ttaaattttt ccttcactac
      241 cacagacacc aataatacga gttacgactt ctttcatggc ggcctttgcg ggctgcatat
      301 agtggcgtgg atcatttgcc gcaggattgt tgtagaaata tccttttaac gcatcggcaa
      361 acgcaatttt caattctgtc gcgacattca ctttacaaat accgcggcga atacagtgct
      421 gaacgtcctg ttctgataag cccgaagcac catgcaacac cagtggaatc tccactcgct
      481 gacgaatctg ttccagccgc acaaaatcca gtcgtggttc atgcgcatat aaaccatgtg
      541 ctgtgccgat tgctacggcc agggagtcaa tttgggtacg ttgaacaaac tctgcagctg
      601 cggctggtga ggtataagcg gaatcttttt catccaccag cagatcgtcc tcctggccgc
      661 caagacgccc tagctcggcc tcaacgctaa catcaaagcg atggcaaaag gtggtaactt
      721 cttgcactcg cgcaatgttg tcttcaaatg gcaaatggga gccatcaatc atgacagaac
      781 ggatccctgc gcgaaccttg ttaaaaatgt catcactctc ttcatgatgg tcgagatgca
      841 ggactaacgg caggttgtac tcgcgagcca gcgattgtac taacgaaatt aaacttccca
      901 cgccaccata acgataagta ccgggtgttc ccgccagcat aacgggagag cgaagttgtg
      961 cagcgctctc aacaactacc tgtaaagttt caaggttatg aatgttgaaa gcggggacgg
     1021 catatccctc tgactgggct tttttcagca tgtttttgct tgaaatgatg tacatcgcag
     1081 ctcctgttat ttgtcgttcg ccttacaaaa tgaagaaaga gaatattcac tttcattttg
     1141 aaagcaaact agcacagcaa aagcggcaaa aagcgtgata catgtcgaat atcatcaaaa
     1201 taacttacaa aaaaataact taatttgttt ttcactttac aagtgaaacc aataacatct
     1261 ctgcataaat atgaataaat tcatttattt ttcaatgaat taatctattt ttgttgcgat
     1321 ttttgaccca tgattttctt ttcgaattgt cgatattatt gaaggataac tttcaaagaa
     1381 aaatacaagg ggctatagtg ttacgcatta aaggccaatg aagagtgtta acgtgccgag
     1441 aaaatcgtct aacctactga aacgcagatt aaaaattgca gaaattgttg ccgcccaagg
     1501 tgaaatcaaa gttgatgact tatctgcgca acttggtgtc tctggcgtga cgatccgcgg
     1561 cgatctcagt tatctggagc aacagggata tttaaagcga tcatttggcg gtgctattgc
     1621 gacaccactc tcctctgaac cagaagcgat acaaccgatc gcgccattgt ctctggcaca
     1681 acaaatggaa atagcacgac attgcgcacg gttaattcgc gatcgtgaca cgctatttct
     1741 tggacatggg acaatctgcc gtaagatcat tccattgctc agcgaagtga aaaaactgcg
     1801 tctgatcacc aacgatcccg aacatgcgct gctggcaaat cagtttattg atggcgaaat
     1861 aatactggct ggcagtgaaa tgctgcgccc cgacaccgca ctggccggaa aaccgcttga
     1921 acaggcactt cagcatttca ccattacaca tagcattctg gaaatcaacc atctggatgc
     1981 tgatggtact ttaaatatca acgttcccag actggccgct gcctggcagc tctgtttcga
     2041 tcatgcccag aataagacgg ttatcgttgc cgccgatcct gcgccatcaa cggctgctat
     2101 cgggcatttg catcaggcag aaaatgtgat cgtcagccgc agcattaacg aacaatatca
     2161 gcaacaattg cagtcggctg atttcgttat cctttatacc agtaatgagt gctttacctg
     2221 gtccaatcgc gaaaggcttc aggaatgaga ggggttttgt gacaataact atctgcgcta
     2281 tgggcgaatt gctggccgag tttttgtccc gcaacccaca tcaaaaattc actcagcctg
     2341 gggagtttat cgggccattt cccagcggtg cgccagcaat ttttgctgct caggtggcaa
     2401 aactgtccca tcgggccatc ttcttcggat gtgttggtaa tgatgatttt ggccgactca
     2461 ttatagagcg cctccgtcat gaaggtgtca ttaccgatgg gatccatgtt atgaacaatg
     2521 ccgtcacagg tacggcgttc gtgagttatc aaaatcccca gcagcgcgat ttcgtcttta
     2581 atatccctaa cagcgcctgc ggcttgttta ctgctgagca cattgataag gatctgctaa
     2641 aacagtgtaa ccatctccat attgtgggct catcattgtt ctcatttcgc atgatcgatg
     2701 tcatgcgtaa agcaataacg acgatcaaat cggctggcgg caccgtttct ttcgatccca
     2761 atattcgcaa agagatgctg agcattcctg aaatggcgca ggctctcgat tatttgattg
     2821 aatatacgga tattttcatc cccagcgaaa gcgaactccc tttcttcgcg cgtcacaaaa
     2881 atctgtcaga agaacagatt gttagcgatc ttctccacgg cggcgtaaaa catgtggcga
     2941 taaaacgcgc ccagcgtggg gccagctatt acaagcttaa aaacggtaca ttacacgccc
     3001 agcatgttac aggccacgat atcgaaatta tcgatccaac gggtgcaggc gactgctttg
     3061 gcgcaacgtt tatcactctt ttcttatccg gtttcccggc acataaggca ctgcaatatg
     3121 caaatgccag cggcgcgctc gccgtaatgc ggcaaggtcc gatggaaggg atatcctcac
     3181 tggctgacat tgaagacttt ttgcagcagc actgaacggc aattttctgt cagattattt
     3241 tccctgcctg aagagttgcc gtagaattgc ccgtttctcg tatcccccca tgaggaggaa
     3301 cccttccgtg gacactcgtt ttgttcaggc ccataaagag gcgcgctggg cgctggggct
     3361 gacccttttg tatctggcag tttggttagc agccgcttac ttacctgacg ttgcccccgg
     3421 ttttaccggc tttccgcgct ggtttgagat ggcctgcatc ctgacgccgc tgctgtttat
     3481 tggactgtgc tgggcgatgg tgaaatttat ctatcgcgat atcccactgg aggatgacga
     3541 tgcagcttga agtgattcta ccgctggtcg cctatctggt ggtggtgttc ggtatctctg
     3601 tttatgcgat gcgtaaacgg agcaccggta ccttccttaa tgagtatttc ctcggcagcc
     3661 gctctatggg cggtattgtg ctggcgatga cgctcaccgc gacctatatc agtgcaagtt
     3721 cgtttatcgg cgggcctgga gctgcttata aatacggcct gggttgggta ttgctggcga
     3781 tgattcagct tcctgcagtc tggctttcgc tcggtattct cggcaagaag ttcgcgattc
     3841 ttgcgcgccg ctacaatgca gtgacgctga acgacatgct gtttgcccgc taccagagtc
     3901 gtcttctggt gtggctggcg agtttgagtt tgctggttgc gttcgttggg gcaatgaccg
     3961 tgcagtttat cggcggtgcg cgcctgctgg aaaccgcggc gggtattcct tatgaaaccg
     4021 ggctgctgat tttcggtatc agcattgcgt tgtataccgc ctttggcggc tttcgcgcca
     4081 gcgtgctgaa cgacaccatg caagggcttg tgatgctgat tggcaccgtt gtgctgctta
     4141 ttggcgtggt acatgccgca ggtggtttaa gtaatgcggt agagacgctg caaaccatcg
     4201 atccgcaact ggttacgcca caaggcgctg acgatattct gtcgcctgcc tttatgacgt
     4261 cgttctgggt actggtgtgc tttggcgtga ttggcctgcc gcatactgcg gtgcgctgta
     4321 tctcttataa agacagcaaa gcggtacacc gggggatcat catcggtacg attgtagtcg
     4381 caatcctgat gttcggtatg cacctggccg gagcgttagg tcgtgcagtg atccccgatc
     4441 tcaccgtacc ggatctggtg atcccgacgt taatggtaaa agtgctgcca ccgtttgctg
     4501 ccgggatctt tctggctgca cctatggctg cgatcatgtc gacaattaac gcccaattgc
     4561 tacaaagttc cgctacgatc attaaagatc tctatctgaa tatccgtccg gatcaaatgc
     4621 aaaacgagac gcgtctgaag cggatgtcgg cggtaattac gttagttctc ggcgcgttgc
     4681 tgctgcttgc cgcctggaag ccgccagaaa tgatcatctg gctgaatttg ctggccttcg
     4741 gtgggttgga agccgttttc ctgtggccgc tggtgctggg tctttactgg gaacgcgcca
     4801 acgccaaagg cgcgctaagt gcgatgatcg ttggcggcgt gctgtatgcc gtactcgcga
     4861 cgctgaatat tcagtacctg ggcttccacc ctatcgtgcc ctcgttacta ctaagtttgc
     4921 tggctttcct ggtcggaaac cgtttcggta catccgtccc gcaagctacc gttttgacta
     4981 ctgataaata aagagttttg ccatgccttg gatccaactg aaactgaaca ccaccggcgc
     5041 gaacgctgaa gatcttagcg atgcgctgat ggaagcgggt gccgtttcta tcacttttca
     5101 ggatacccac gatacgccag tatttgagcc gctgccgggc gaaacgcgcc tgtggggcga
     5161 caccgatgtg attggtctgt tcgacgctga aaccgatatg aacgacgtgg tggcgattct
     5221 ggaaaaccat ccgctgctcg gcgcaggctt cgcgcataaa atcgaacaac tggaagataa
     5281 agactgggag cgcgaatgga tggataattt ccacccgatg cgctttggtg aacgactgtg
     5341 gatctgccca agctggcgtg atgtgccgga cgaaaacgcc gtcaacgtga tgttagaccc
     5401 agggctggcg tttggtacgg gtacccatcc aactacctct ctgtgcctac aatggctcga
     5461 cagcctcgat ttaactggta aaacagtcat cgactttggc tgcggttccg gcattctggc
     5521 tatcgcggcg ctgaaactgg gtgcagcaaa agccattggt attgatatcg atccgcaggc
     5581 gattcaggct agccgcgaca acgccgaacg taatggcgtt tctgaccgtc tggaactcta
     5641 cttaccgaaa gatcagccag aagaaatgaa agccgacgtg gtggtcgcga acatccttgc
     5701 aggcccatta cgtgaactgg caccgttaat cagcgtcctg ccggtttcag gcggtttgct
     5761 gggcctttcc ggtattctgg caagccaggc agagagcgtt tgtgaagctt atgccgatag
     5821 cttcgcactg gacccggtcg tggaaaaaga agagtggtgc cgtattaccg gtcgtaagaa
     5881 ttaaccttcg catcgccgta gggtgacgcg gggcgaagtg cgagcaagct cacaaaaggc
     5941 acgtaaattt gccggttatt tacgcaaatt tgcgtgccaa aattttcatt cataaagaaa
     6001 aattgagaac ttactcaaat ttctttgaat gtaaatttta gtcactattt tctaatatga
     6061 tgatttttat gagtaattat cgcaccacgc tcattttaaa tgcaattctt tgatccatct
     6121 cagaggattg gtcaaagttt ggcctttcat ctcgtgcaaa aaatgcgtaa tatacgccgc
     6181 cttgcagtca cagtatggtc atttcttaac tcatgcgcat cggacaatat cagctcagaa
     6241 atcgcctgat cgcagcgccc atggctggca ttacagacag accttttcgg acgttgtgct
     6301 acgagatggg agccggattg acagtatccg agatgatgtc ttctaaccca caggtttggg
     6361 aaagcgacaa atctcgttta cggatggtgc acattgatga acccggtatt cgcaccgtgc
     6421 aaattgctgg tagcgatccg aaagaaatgg cagatgcagc acgtattaac gtggaaagcg
     6481 gtgcccagat tattgatatc aatatgggtt gcccggctaa aaaagtgaat cgcaagctcg
     6541 caggttcagc cctcttgcag tacccggatg tcgttaaatc gatccttacc gaggtcgtca
     6601 atgcagtgga cgttcctgtt accctgaaga ttcgcactgg ctgggcaccg gaacaccgta
     6661 actgcgaaga gattgcccaa ctggctgaag actgtggcat tcaggctctg accattcatg
     6721 gccgtacacg cgcctgtttg ttcaatggag aagctgagta cgacagtatt cgggcagtta
     6781 agcagaaagt ttccattccg gttatcgcga atggcgacat tactgacccg cttaaagcca
     6841 gagctgtgct cgactataca ggggcggatg ccctgatgat aggccgcgca gctcagggaa
     6901 gaccctggat ctttcgggaa atccagcatt atctggacac tggggagttg ctgcccccac
     6961 tgcctttggc agaggttaag cgcttgcttt gcgcgcacgt tcgggaactg catgactttt
     7021 atggtccggc aaaagggtac cgaattgcac gtaaacacgt ttcctggtat ctccaggaac
     7081 acgctccaaa tgaccagttt cggcgcacat tcaacgccat tgaggatgcc agcgaacagc
     7141 tggaggcgtt ggaggcatac ttcgaaaatt ttgcgtaaac agaaataaag agctgacaga
     7201 actatgttcg aacaacgcgt aaattctgac gtactgaccg tttctaccgt taactctcag
     7261 gatcaggtaa cccaaaaacc cctgcgtgac tcggttaaac aggcactgaa gaactatttt
     7321 gctcaactga atggtcagga tgtgaatgac ctctatgagc tggtactggc tgaagtagaa
     7381 cagcccctgt tggacatggt gatgcaatac acccgtggta accagacccg tgctgcgctg
     7441 atgatgggca tcaaccgtgg tacgctgcgt aaaaaattga aaaaatacgg catgaactaa
     7501 ttcaggttag ctaaatgctt gattaaaaag gcgctactcg gcatggggaa gcgccttttt
     7561 tataggtgtc acaaagggag tgaccatgag aacaggatgt gaaccgaccc ggtttggtaa
     7621 tgaagctaag accattattc acggtgatgc gcttgccgaa cttaaaaaga tccccgccga
     7681 aagtgtcgat ctgatctttg ccgacccacc atataacatc ggtaaaaatt ttgatggtct
     7741 gatcgaagcc tggaaagaag atctgtttat cgactggctg tttgaagtga ttgcagagtg
     7801 ccaccgcgtt ctgaaaaagc agggcagcat gtacatcatg aacagcacgg aaaacatgcc
     7861 ctttatcgat ctccagtgcc gcaagctttt taccatcaaa agtcgcatcg tctggtcata
     7921 tgacagttct ggagtacagg cgaaaaaaca ctacggctcc atgtacgacc ccatcctgat
     7981 gatggtgaaa gacgcaaaga actacacatt caacggtgat gctattctgg tagaagccaa
     8041 aaccggatcg cagcgcgcgt tgatcgatta tcgcaaaaat cctccacagc catacaatca
     8101 tcaaaaagta ccgggtaacg tctgggattt tccgcgcgtg cgttatttga tggatgaata
     8161 tgagaaccac ccgacgcaaa aaccggaagc cttactgaaa cgcattattc tcgcctcttc
     8221 caacccaggc gatatcgttc tcgacccgtt tgccggtagc tttactaccg gtgccgtagc
     8281 cgtctccagc gggcgaaaat tcattggtat tgagatcaac agcgagtaca tcaaaatggg
     8341 gcttcgacgg ctggatgtcg cgtcgcatta ctctgcggaa gaactggcga aagtgaaaaa
     8401 aagaaagacc ggcaacctgt caaaacgaag ccggttaagc gaagttgacc ccgatctcat
     8461 tgcaaagtaa aggaagtgta agcctggttt tcagattatt catttcgtgt atatttcctg
     8521 ccagacttgg ttaaacatgc acaggctcag gtaatgattc gcaagtattg gtggctcgtc
     8581 gttttcgctg tcttcgtttt tctgtttgat actttactga tgcagtggat tgaactgctg
     8641 acaacagaaa cagacaaatg ccgtaatatg aactcagtta atccactaaa actggttaac
     8701 tgtgacgaac tgaattttca ggacagaatg tgaatttaca tgacacttaa ttcattcgtt
     8761 tgatgaatta atttcgttat gttttcatct ggcatgaaca ttcttaatac gttatcgacc
     8821 agcgcgggtg cttgtttata aagatcataa cccgccatat tcattaacca gttttgaaca
     8881 attccgctga aggcaccatt aataataatc atcacaacat ctaaatcgag gttatttgct
     8941 acacaacctt gttgctgaca cgcctgcaat acttcgcgga gagtctgcgg attaaagccc
     9001 atcttttcgc gtatcactcc ctcggccagc atctcatcat taaattcaca tttgtgatat
     9061 aagattttca gcaacgcctg ctggcgggga attttggcaa tatattgcaa gccgacaatc
     9121 aatttttcac gcaattgttg aaacgggtca tgctctaatc cagccgtcaa gtgttcctgg
     9181 attaactccc gcaatgaagg ctgttgcaac cacatctcat taaacagttg agtcttgttt
     9241 tcgaagtgcc agtagatagc gccacgcgta acgttagcgg cgtcggcaat gtcgttgagc
     9301 gtcgtcttgc ttacgccatg ctgcgcaaac tgggcgatgg cagtttcaat cagttcttgc
     9361 cgggtcttca gagcttcggc tttggttctt tttgccatga ttaattattc aggaaataaa
     9421 tatattcgac acagggtgag aaaatagcga aggttaatct atcatctaat gtgtatttat
     9481 acgagagggt aacattgagt tgctataaat cgtcaaataa acaatgtata ttatttacct
     9541 aaaatacatt cgccacatga atatatattt caa
//
LOCUS       DAJTNN010000161         9490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTNN010000161 DAJTNN010000000
VERSION     DAJTNN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.161"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..881
                     /gene="potE"
                     /locus_tag="OO150_003363"
     CDS             <1..881
                     /gene="potE"
                     /locus_tag="OO150_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5517398.1"
                     /translation="QISSITVWGVIIPVVGLCIIGWFWFSPTLYANSWNPHHVPFFSA
                     VGSSIAMTLWAFLGLESACANAEVVENPERNVPIAVLGGTLGAAVIYIVSTNVIAGIV
                     PNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEG
                     YFPKIFSRVTKVDAPVQGMLTIVIIQSGLALMTISPSLNSQFNVLVNLAVVTNIIPYI
                     LSMAALVIIQKVANVPPSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFLGWT
                     LYGLVSPCFELKNKHG"
     gene            957..1106
                     /locus_tag="OO150_003364"
                     /pseudo
     CDS             957..1106
                     /locus_tag="OO150_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            1230..1880
                     /locus_tag="OO150_003365"
     CDS             1230..1880
                     /locus_tag="OO150_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HCR5517399.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(1921..2415)
                     /locus_tag="OO150_003366"
     CDS             complement(1921..2415)
                     /locus_tag="OO150_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517400.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     RLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(2630..4270)
                     /gene="pgm"
                     /locus_tag="OO150_003367"
     CDS             complement(2630..4270)
                     /gene="pgm"
                     /locus_tag="OO150_003367"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5517401.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMEAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(4296..4841)
                     /gene="seqA"
                     /locus_tag="OO150_003368"
     CDS             complement(4296..4841)
                     /gene="seqA"
                     /locus_tag="OO150_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5517402.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5026..5790
                     /gene="ybfF"
                     /locus_tag="OO150_003369"
     CDS             5026..5790
                     /gene="ybfF"
                     /locus_tag="OO150_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5517403.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            5930..6223
                     /gene="ybfE"
                     /locus_tag="OO150_003370"
     CDS             5930..6223
                     /gene="ybfE"
                     /locus_tag="OO150_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5517404.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            6363..6893
                     /gene="fldA"
                     /locus_tag="OO150_003371"
     CDS             6363..6893
                     /gene="fldA"
                     /locus_tag="OO150_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5517405.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7103..7189
                     /gene="uof"
                     /locus_tag="OO150_003372"
     CDS             7103..7189
                     /gene="uof"
                     /locus_tag="OO150_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5517406.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7182..7628
                     /gene="fur"
                     /locus_tag="OO150_003373"
     CDS             7182..7628
                     /gene="fur"
                     /locus_tag="OO150_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5517407.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(7713..8039)
                     /locus_tag="OO150_003374"
     CDS             complement(7713..8039)
                     /locus_tag="OO150_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517408.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFPDQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8089..>9490)
                     /gene="chiP"
                     /locus_tag="OO150_003375"
     CDS             complement(8089..>9490)
                     /gene="chiP"
                     /locus_tag="OO150_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5517409.1"
                     /translation="TFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGGIY
                     YWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAENGD
                     SSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLLAP
                     HWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKVDY
                     LHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRDKV
                     DDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPAYASSN
                     GRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQSNP
                     DAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNIFQ
                     DERDVKFMVIAPFTIF"
BASE COUNT         2314 a         2350 c         2349 g         2477 t
ORIGIN      
        1 ggcaaatcag tagcattact gtgtggggag tcattattcc ggtcgtcggt ctgtgcatta
       61 ttggctggtt ctggtttagt ccgacgctgt acgctaactc atggaacccg caccatgtgc
      121 cgttctttag cgctgtaggt tcttccatcg ccatgacgtt gtgggctttc ctcggtctgg
      181 agtcagcctg tgccaacgcc gaagtagtgg aaaacccgga acgtaacgtg ccgattgcag
      241 ttcttggcgg tactttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgccg
      301 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gctttcgcac
      361 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
      421 gttcactgct tggctggcag ttcaccattg cccaggtgtt taaatcttca tctgatgaag
      481 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
      541 tgaccattgt gattattcag agtggattgg cactgatgac cattagcccg tcactgaaca
      601 gccagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
      661 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
      721 tagcaaactt tgttgccttt gttggtgcga tgtacagttt ctatgcgctg tactcatccg
      781 gggaagaagc catgctgtac ggttctatcg tgaccttcct tggttggaca ctgtatggtc
      841 tggtatcacc atgctttgaa ctgaaaaata aacacggtta ataaaacaaa gtaaaaaagg
      901 gcgattgaat gatcgccctt tctgtttgtt ggcaaatagt tcgttattta tgccgcatcc
      961 ggcagatgta gggtctaaac gcgatcgaac acagcaacta tttgcgtaat tatatggggc
     1021 atctgcgaca aaaactggaa caggatccgg cccggcctcg ccattttatt actgaaaccg
     1081 gtattgggta tcggtttatg ctttgaaaaa agtggcaatg tatggataaa gtcatttttt
     1141 attcaaatat aaggaactta atatttaaaa aatgttccat acaattcctc atcataaatg
     1201 acaccgtagc acacaaggag caagtagcga tgcgtgaaat gaatcagtta gtaaatactg
     1261 aagattcagc ctggccaata atccagaact ggttaaaaga cgccacaaac cataccgaac
     1321 tcttaccggt taataaaaat ttagccgaaa ccgcacttta tcaattacag gtgacaacaa
     1381 aatcaccgat gggtgcgctt gtctatggct ccggaggtct gctgattgat aatggttggt
     1441 tacgcatagc aggatctggt cacccccggt tgccacgaga tccagtaagt tggacacaac
     1501 gccctgaatt cgcaggcgtg cgggctttac ccatcgctga cgatgtcgca gggggaattt
     1561 ttgctcttaa tggtggcgat cttggggaag atactggctg cgtttattat tttgcgccag
     1621 atacgctgaa ttgggaaagt ctggaagttg gatattctga gtttcttcag tgggcattat
     1681 ccggtgattt agataccttc tatgaaaatg tacgatggca gcaatggcga gaagacgtaa
     1741 taaaattatc ggcaacggaa gccttcacgt tctacccttt cttatgggta caaagtgaag
     1801 aagcaagaac aagaaaagtt atttcgctta ctgaactctg ggaaatgcag tatcaaatga
     1861 aagaaacatt cacgcaataa gacatgttca ggtccgggtt tcattcccgg actcactatt
     1921 ttaatgtgta tttatcgctt tcaattcttg ctggcaattt tctgactggc agcgtcgtaa
     1981 ctcaataatc ccgacatgca tcatcgagcc aaaaaatccg ctttcgctga cataatcctc
     2041 attgactatc ggtcgagtgc cgggcttgcc ctggcaaacc cgcataaaaa atgaggtgta
     2101 gcattgccaa ccgggattta aaccgacctt ttcttgtgct gacatattat gaagataagt
     2161 aagttctttt tcgttcccgc ctgacaactg ccagcggcgg gtattatcag gtaagttatc
     2221 accacggcaa tttgtcttac accgggaagg tgcaatattt accgcaatgc tacccgttaa
     2281 tcttctggtt ttatctttag gggtcagtaa ccagtcgcca gctaatgatg attgttcagg
     2341 ttgggcgcat gcagtcagca gtaatgtggc ggtaattagt gacactatga cgaccagact
     2401 cctgttggtc ttcattggga tttccttgag ctactcagtt tgttgacaaa gtgttcgttg
     2461 tttttgccgg atgcggcgtg aacgccttat ccggcctaca aaatctcgta aattcaatat
     2521 attgcaccat cctcgtaggc ctgataagcg tagcgcatca ggcaattctg tgtttgtcat
     2581 acgtaaaaaa gggcgatctt ccgaccgccc tttttcttat ttaatgtgtt tacgcgtttt
     2641 tcagaacttc gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga tgttcttcac
     2701 cgaggaagct ttcgcagtag atcttatatg cgtcttccgt gcctgacgga cgcgcggcga
     2761 accagccgtt gtcagtcatc actttcagac cgccgataga agcaccattg cccggtgccg
     2821 ccgtcaggcg cgcggtgatc gggtcgcctg ccagggtgct ggcgctcacc atttccggag
     2881 acagcttaga cagcgccgct ttttgtgcgg aagtcgcagc tgcctgcaaa cggttgtagc
     2941 tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc ctgcgggttc ttaccggtga
     3001 cagcggtgat ttccgccgcc agcagacaca tgatgatgcc gtctttgtcg gtggaccacg
     3061 gcgtgccgtc gaaacgcagg aaggaagccc ctgcgctctc ttcgccgcca aagccgaagc
     3121 tgccgtcgaa cagaccatca acaaaccatt tgaaacctac cgggacttct accagcttgc
     3181 ggcccaagtc attgaccaca cggtcgatca tcgcagaaga aaccagcgtt ttaccgacag
     3241 caacatcttt gccccactgc ggacgatgct ggaacaggta gttgatcgcc accgccaggt
     3301 agtggttcgg attcatcaaa cctgccggag tgacgatacc gtgacggtca taatccgggt
     3361 cgttagcaaa cgccagatcg aacttatcac gcagtgccag caggcccgcc atcgcacact
     3421 cggaggagca gtccatacgg atcgcgccgt ctttatcaag gtgcataaag cggaaggttt
     3481 gatcgacctg atcgttaacg atggtcaggt tgaggttgta atactcgcca atacgcttcc
     3541 agtattcgat accggaaccg cccagcggat caacgcccag cgtcaggccc gctttctgaa
     3601 tcgcctccat atcaacgata tcggccagac cttccacaaa cggctgcacc agatcctgct
     3661 ctttcacatg accggatgcc atcgcttcgt cgagggagat acgcttcacg cctttcaggc
     3721 catcggccag cagtgcgttg gccctgtctt ccaccacttt ggtgacgttg gtatcagccg
     3781 ggccaccatt tggcggattg tacttgatac caccatcttc cggcgggtta tgggacggtg
     3841 taatcacgat accgtctgcc agcggaccac cttttttatt gtgaaccagg atggcattgg
     3901 aaacggcagg cgttggagtg aagccattgt tttcctgcac aatgacatca acgccgttcg
     3961 ctgccagcac ttccagtacg gaaatgaacg caggctcgga cagggcgtga gtatctttac
     4021 ccacatagca agggccagtg atgccgtttt tcgcacgttc ttcagcaatt gcctgagcga
     4081 tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg tgaccggaag
     4141 taccgaattt caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca taatattgcg
     4201 ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc aggttggcct gcacgattgt
     4261 ggattgccat tgctttgtcc tttgtctgca acgttttaga tagttccgca aaccttctca
     4321 atcaattccg ccgggaattg catcgactgc atgatgtgtt cgatcatgct gcatttacgg
     4381 ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg gcacatgttt cggcttggtc
     4441 tgattaccat ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg tgtacgaccg
     4501 tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag tgtagacaac
     4561 agcagcataa agcgattgac cgctcgcttt tgctctgcgt attcgtcaga aagcagaagt
     4621 tcacgcattg cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc gacgatagca
     4681 ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag gctgtgatgc ggcggaaaat
     4741 ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt gtggctggca
     4801 atatagctgt agagttcatc atcaacttca atcgttttca tcttaatcca gtgcagtgtc
     4861 ttagctgaat acaagtcgcc aagagtatat gaataaatcc cggcagcgga tagcgtcaag
     4921 cctggcttgc agaaaaactc ggaataaacc ttatttgttg cagccctgtg gcacaatggt
     4981 caacataata ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa attgaatatc
     5041 cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca tcgttcttgt ccatggtctg
     5101 tttggtagcc tcgacaacct tggcgtactg gctcgcgatc tggtaaacga tcacaatatc
     5161 atccaggttg atatgcgtaa ccacggtctt tcaccgagag atccggtaat gaattacccg
     5221 gcgatggcgc aggatcttgt tgatactctg gatgcacagc agatcgacaa agcaacattt
     5281 atcggtcact ccatgggcgg taaagcggta atggcactta cggcactggc ccgcgatcgc
     5341 atcgataaac tggtggcgat cgatatcgca ccggtcgact atcacgtacg ccgtcatgat
     5401 gagattttcg cggctatcaa cgcggtcagt gaatcggacg cacaaactcg ccagcaagca
     5461 gcagcaataa tgcgccagca tcttaatgaa gaaggggtga ttcagtttct gttgaaatct
     5521 tttgttgacg gggagtggcg ctttaacgtg ccggtattgt gggatcagta tccgcatatt
     5581 gtgggttggg agaaaatccc ggcatgggat caccctgccc tgtttatccc tagcggcaat
     5641 tctccgtatg ttagcgagca gtaccgtgat gatttactgg cacaatttcc acaggcacgg
     5701 gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga tgcggtatta
     5761 cgcgctatcc gtcgctatct caatgattaa cgcaactgat taaaaaccca gcgtccgcgt
     5821 cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg atgtattatg gcgcgctatc
     5881 catccgggca gaggcctggc tgattgtttc ccccgaagtc accaagatca tggccaaaga
     5941 acaaacggac cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg gacgaccgaa
     6001 aactaatccg ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc agctaaaacg
     6061 cgacaaagta cgtggcctta agcgtgtcga actgaagctg aacgcggaag ctgtcgaggc
     6121 gctgaacgag ctggcggagt cgcgcaatat gagccgtagc gaactgatcg aagagatgtt
     6181 aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca ctttcatgta
     6241 gcacagtgtg cagtcctgct cgtttgctga ttccgaaaga ctgcctgttc tgctatgatt
     6301 gcctttatcc gtgggcaatt ttccaccccc atttcaataa gtttcaagag gttatttcac
     6361 tcatggctat cactggcatc tttttcggca gcgacaccgg taataccgaa aatatcgcaa
     6421 aaatgattca aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt gcaaaaagca
     6481 gcaaagaaga tctggaagct tatgacattc tgctgctggg catcccaacc tggtattatg
     6541 gcgaagcgca gtgtgactgg gatgacttct tcccgactct cgaagagatt gatttcaacg
     6601 gcaaactggt tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa tatttctgcg
     6661 acgcattggg caccatccgc gacatcattg aaccgcgcgg tgcaacgatc gttggtcact
     6721 ggccaactgc gggctatcat ttcgaagcat caaaaggtct ggctgatgac gaccactttg
     6781 tcggtctggc tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt gtagaaaaat
     6841 gggttaaaca gatttctgaa gagttgcatc tcgacgaaat tctcaatgcc tgatgtgatg
     6901 cggcgtagac tcatgtctac gccgtattaa tagataatgc caatcaaaat aattgctaca
     6961 aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg gccttgccgt
     7021 tgtaaatgta agctgtgcca cgtttttatt aacaatattt gccagggact tgtggttttc
     7081 atttaggcgt ggcaattcta taatgatacg cattatctca agagcaaatt ctgtcacttc
     7141 ttctaatgaa gtgaaccgct tagtaacagg acagattccg catgactgat aacaataccg
     7201 ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt aaaaatcctg gaagttcttc
     7261 aggagccgga caaccatcac gtcagtgcgg aagatttata caaacgtctg atcgatatgg
     7321 gtgaagaaat tggtctggct acggtatatc gcgtactgaa ccagtttgac gacgctggta
     7381 tcgtcacccg ccacaatttt gaaggcggta aatccgtatt tgaactgaca cagcaacatc
     7441 accacgatca cctgatctgc ctcgactgcg gcaaggttat cgaatttagt gatgattcca
     7501 tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat tcgcctgact aaccacagtc
     7561 tctatcttta cggtcactgt gccgaaggcg attgccgcga agatgaacat gcgcacgaag
     7621 gcaaataagc cagcctgaat gagaaaaagc caacctgcgg gttggctttt ttatgcaagg
     7681 gaaataagaa aagcgagagt tacagctctc acttatttgt tattactgcg aatttcctgc
     7741 caaactttat cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga
     7801 cactgctgat aatccagtac gcggacactt tcctggtcgg gaaaatgctg atgttgtttc
     7861 tctttcttca gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgt
     7921 gcggtgttga tacaggcgct gtatgcctct ttcagacgac tgtcttcctg tggcgcggtt
     7981 gattgcgcac aagctaccag ccccgatgcc attatggcga tgagaatcag ttttttcata
     8041 gcaaattcct caaacgtgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt
     8101 gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaagatgt tgccgtaacc
     8161 accgccccag cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat
     8221 cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt atgcagactc
     8281 ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgccagg tcgcaggttt
     8341 agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt
     8401 caggtcatac atcgcaccga agaagaccgc tttttcgccg ttagcgttga agtcggaacg
     8461 gttatcccac cagatatcca ggcggccgtt ggaggaagcg taggctggag tcatacgttg
     8521 caggaagtat ccctgctgac cgtcagcctt aacccaggtg ccttcgaggc gcaaatccac
     8581 tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag
     8641 gtcgttgacg ctgcgatcgt caactttatc gcgcgtacca tagaactggt agctggtggt
     8701 taacgggcta ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata
     8761 cccttccgcc tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc
     8821 cccaagggag tgcagataat caactttggt ggttttatcg ttctgataaa actcatccat
     8881 ttccagatgc cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaacgcacc
     8941 agcatcgccg taatcaaaat tcgccccggc ttccgccccc tgataagtac ctggcataaa
     9001 gctccagtgc ggcgctaaca gcgtttgacc agttggctga atgtaacctg ccctcgccca
     9061 aaccggaccg tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc
     9121 agaccagtct tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga
     9181 gctgtcgccg ttttccgcca tttcaatcgc cgtaaacaca gcaatatcaa ggccgaacat
     9241 atcagcagca taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt
     9301 ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata
     9361 tataccgccg gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc
     9421 cataaagccc gacattgctg taacaccggc gatagccagc gccagcgtac tacgtttgcc
     9481 actaaacgta
//
LOCUS       DAJTNN010000162         9486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTNN010000162 DAJTNN010000000
VERSION     DAJTNN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.162"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            6..81
                     /locus_tag="OO150_003376"
     tRNA            6..81
                     /locus_tag="OO150_003376"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO150_003377"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO150_003377"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1225)
                     /locus_tag="OO150_003378"
                     /pseudo
     CDS             complement(468..1225)
                     /locus_tag="OO150_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(1233..2336)
                     /locus_tag="OO150_003379"
     CDS             complement(1233..2336)
                     /locus_tag="OO150_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5517410.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     VLLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2346..3516)
                     /locus_tag="OO150_003380"
                     /pseudo
     CDS             complement(2346..3516)
                     /locus_tag="OO150_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3584..4645)
                     /locus_tag="OO150_003381"
     CDS             complement(3584..4645)
                     /locus_tag="OO150_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5517411.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LLGFSYADADGKFSGIDVDVDVDVDVDVDVDVCRSVAAAVFGDDTKVKYTPLTAKERF
                     TALQSGEVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGAT
                     VCIQAGTDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALR
                     IKLSNPAEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDE
                     KAANPATPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIK
                     RGQNNLWNNGGIQYAPPVR"
     gene            complement(5076..5297)
                     /locus_tag="OO150_003382"
     CDS             complement(5076..5297)
                     /locus_tag="OO150_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517412.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGCLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIAMSK"
     gene            complement(5550..8654)
                     /gene="acrF"
                     /locus_tag="OO150_003383"
     CDS             complement(5550..8654)
                     /gene="acrF"
                     /locus_tag="OO150_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCR5517413.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTNDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVL
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALSV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFLPVFFVVIRR
                     CFKG"
     gene            complement(8666..>9486)
                     /gene="acrE"
                     /locus_tag="OO150_003384"
     CDS             complement(8666..>9486)
                     /gene="acrE"
                     /locus_tag="OO150_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR5517414.1"
                     /translation="SEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVI
                     AAKGTVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDV
                     TQSSNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSI
                     TLRAVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQV
                     EARPVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2523 a         2461 c         2274 g         2228 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcaaattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtcttcc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggcccagt cttccgactg agccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc tcgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgttcca ctatctcccc acgatccata aaaattaccc ggtcagcgac ggtacgtgca
      601 aaccccatct catgtgttac acacaacatt gtcatacccg actgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgaggccga cgttggttca tcaaacaaca
      721 taattttcgg cttcatacac agcgaacgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttcttaggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtaa aacggtcaga tgagggaaga gattgaaatg ctgaaatacc attcccactt
      961 cttgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctacgacaa
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aaccagaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat ggacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgttggct ataacgtgac atgctgaaac aaaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg cgggatcaac ggttgcctgc
     1381 tgaacgctac tgaaaagatc gaacaagccg atgatgatca ccagactggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaccccaac gccagcgact ctgccgcttc atattgccct
     1561 ttaggtaggg cctgtaatcc gcctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcagtttg tcgatactgg tgccttctgc cataaacaac
     1681 ggcagcatga ccgaagacat aaacaatacg gtaatcagcg gtacgccgcg ccagaactcg
     1741 ataaaaatga ccgataagat acgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaacggatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tcaagaccaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcaccgcc cagaccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgattgac acaaggccaa tcagtaatac taggttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagtcca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccagttagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggcttgagt tatggctcgc tgggcgcgag ggctgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttcaacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc
     2461 gtctggttca gcacggtgcc agcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggcgg aataatcact
     2581 cgcagtgcct ggggaataat gacctggcgt agcgtaaccc cagtgaccga gccgcttcat
     2641 gttgaccata aggcactgcc tggatcccag cgcggataat ctcggcgata aatgcagagg
     2701 tataaaccga aagtgccagc gttaaggctg ccagttcagg aattaaaacc attccgccgc
     2761 ggaagttaaa gcctcgtagg gctgggacat cccagtgtag tgctgcgcca aaaagccatt
     2821 gcgccagtaa aggcaaaccg atgatcaaca ctgcggcgaa cggccaggtg cggcgcagtt
     2881 gtccggtctt tatctggtat gttttattaa agcggaatag cccaacagaa aggactatag
     2941 ccataacaac agctaggata aacgcaataa atccgtctcc cagctgcggt gacggaatat
     3001 aaagcccacg attgctcaaa aaggccagat caaacgcgct cacagcctgg cgcgtggtcc
     3061 gggcaaattg cgcaacacgg caaagtacca gaagaagatt tgcagcagcg ggggaatatt
     3121 acggaagatc tcaatataaa ttgtggatag ctttcgtagc agtcagttat ccgaaagtct
     3181 cgtcagaccg ataaagaagc ctaaaacaga agcgaacaca atacacaatg cagaaaccag
     3241 tagcgtattg agtaagccga caataaaaac gcgtccgtag gtgtcgccct gctggtaatc
     3301 gatcaaatgc tggacaatac cgaagccagc gccgcgatcc agaaaggcaa aacctgaagt
     3361 aatgccacga ttactgagat tcgttacagt gttgtgaaat aaccaaccaa caattccgac
     3421 aacagcaaca acggcaagga tctggaataa ccaggcacga accgtagggt tggcaaagga
     3481 gagtgagcct ttaacggttg agcggcgatg agacatagaa acctcggaaa gcataacgct
     3541 gaagagagcg ccgccgtagc gacgctcgtt acctcacacc gacttaacgc acgggcggcg
     3601 cgtactgaat accgccgtta ttccagagat tattttgccc acgtttaatt ttcagcgggc
     3661 tttctgaacc tacgttacgc tcgaaaattt ccgagtagtt acccacctgt ttgatgatgt
     3721 tataggccca tttattatcc aacttcagat ccttgccgta atcgccttct ttacccagca
     3781 gatgtgccat atcaggcgtt gctggattag ccgctttttc atcgacgttc tgggaattga
     3841 tgcccatctc ttcagcattc agcatggcga aaagcgtcca gcgtacaatc gagaaccatt
     3901 catcatcgcc acgacgaact accggcccaa gcggttcttt agagataacc tccggtaaga
     3961 caatccattc agctgggttg cttaatttga tgcgcagggc atacagttgc gactgatccg
     4021 aggccagcgt atcgcagcga ccagattcca gtgccttcgc tgattcgtca gagcgatcga
     4081 aagtcaccgg tgtgtacttc atattgtttg ccttgaaata gtcggcaacg ttgagttcgg
     4141 tatcagtacc cgcctgaata cagacggtag cgccatccag ttctttcgcg ctttttagcc
     4201 ccgctttatc gtgcgtcagg aagccaatgc cgtcgtaata agtgacgcca gtaaatgcca
     4261 ttcccatccc ggcgtcgcga gatgaagtcc aggtcgtatt acgggagagc aaatccactt
     4321 cccctgactg taaagccgtg aagcgttctt ttgctgtgag tggggtatat ttcactttcg
     4381 tgtcgtcacc aaatacagca gcggcaacac tacgacaaac atcaacatca acatcaacat
     4441 caacatcaac atcaacatca acatcaatac ctgaaaactt accgtctgca tcggcataag
     4501 agaacccaag taatccatca ctgatcccgc attgcacaaa accttttttc tgaacggcat
     4561 caagcgtcgc gccagcatgc gcctgatttg caacggcaag cagcacgctg gcggcagcca
     4621 gtgtggctat catcatcttt ttcataatgc ttcctgtgag gcgaaaatta tcgttatagg
     4681 acgtttggga aacgtctttt acctgtctgt ggccatcctc ttataagcaa aagtagtgcc
     4741 agcttttgcg cacgatttcg cgtcgcaata agctgagtgt agacaggaaa taataaaaac
     4801 agcgccccgc aatggtgcga aaatgaaaca tgcgccacat tttggcgcaa aatatttacg
     4861 cgtgttttac taagcagaat aatgattgtt agcgtgagta tatttctggt gggaagtgtg
     4921 aaagttaaat tgatgcgtta tttcgaaagc agcgaaataa cgcgatataa catgctaaat
     4981 cagcagagaa ttagccagca gaatatagca atattttatc ttatgtaaag atactgtatc
     5041 gttaaacaga gcgcgaaact ccgcgccctg ttcagttatt tcgacatagc gatgatcccc
     5101 atcgtcaggc cagcaacagc cgctgccccg ccggcaatag ccccagccgt ttcccagtta
     5161 tcctgagtcg tgcctttcat acaggtattg gtgtgaacca gacaaccctg atcgtcataa
     5221 accggaacgc agggcgaatc gtgagcacag cccgccagca atgcggtgag caatgccaca
     5281 ggaattaatc ttttcatggt gttacctcag taataaacgc taacgcttaa taagttaact
     5341 ctcttattaa cgagctgaat gccattttat caccggaggt tataaatatt gcggaatgaa
     5401 taatgacaaa ttatgtgaat tgtaaaaata gtgaagggag cgggaactat agaaagtttt
     5461 taaagagtga gtggaagaat atcttattaa tttttatggg tttcactgaa aataataaag
     5521 gcacccgaaa gtgcctttat gtttctgatt tatcctttaa agcaacggcg gatcaccaca
     5581 aagaacaccg gtaggaagaa gattgccagc aacgttgcag agaccattcc gcccattacc
     5641 ccgataccca ctgcgttctg cgcgccactg cctgcaccgt tactgatagc tagcggtaat
     5701 acgccgagaa taaaggcgag agaggtcatc aggataggac gcagacgcat acgtactgcc
     5761 attagtgtcg cttcaacaac acctttaccc tctttctcca tgagatcttt agcgaactca
     5821 acaatcaaaa tagcgttttt ggccgacaag ccaattgtcg ttagcaagcc caccataaag
     5881 tagacgtcat ttttttgatt aaagagtgtc gccgccagca gcacgccgac aatccctaac
     5941 ggcacgacca acataaccga gacaggaatt gaccagcttt catagagtgc agcaaggcac
     6001 aggaaaacaa ccacaaagga aattgctact agagcgggag cctggtttcc cgataagcgt
     6061 tcctgatacg acatacccgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
     6121 ttttccatca acgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
     6181 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
     6241 gtggtaaagg ctgagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
     6301 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
     6361 ccacggtcga tgaagtcgtt aacgtaagtc ccacccaacg ccgttgaaat ggtctgattg
     6421 atgtcagaaa gtgaaacact taatgcctgc gccttttcct ggtcaacttc cagtttaaac
     6481 tgcgcggtgt cttccaggcc attaggacgc acgctgacta agctggcagg atgttgcgcc
     6541 gccataccaa gcaactggtt acgggcctgg gttagggcat cgtgacccag cccagcctga
     6601 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
     6661 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
     6721 gcttccgcac tgttttcatc accattacgc tcttcccacg gtttcagact gacgaaggcc
     6781 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttca
     6841 acgttcgctt tctcgttctt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
     6901 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
     6961 tcaggtaaga aggaagacgg aagacgtaaa aacaacacca ccattcctgc aacaatcagc
     7021 gcatagatca gtaaatatcg tcctgtggaa ccgaggattt tgccgacgct gttggtgtag
     7081 tggttaacgc tatgatcgaa ggtggtatta aaccagccga agaaaccgcc cttattttcg
     7141 tgatgctcag cagagacggg tttaagcagc gttgcacata acgcaggggt aagaatcaat
     7201 gccaccagaa cagaaagtgc cattgccgaa acgatggtga tagagaactg gcgataaatt
     7261 gccccagtag aaccgccgaa gaatgccatc ggaataaata ccgctgacag caccatcgcg
     7321 atacccacca gtgcgccctg aatttgcgac atcgattttt ccgtcgcttc ttttggcggg
     7381 agcttatcct ccatcatcac gcgctcgacg ttctccacca ccactatcgc atcatcgacg
     7441 agcagcccga tggcaagcac catcccgaac atcgttagtg tgttgatgga gtaaccaaaa
     7501 gcggcgagga tggcaaacgt ccctaacaac accacgggta ccgcaatggt ggggatcagc
     7561 gttgctcgca tattctgcaa gaacagatac atcaccagga acaccagcat aatggcttcg
     7621 aacagcgttt ttaccacttc gtgaatagaa agcaggacga atggcgtggt gtcataaggg
     7681 tagagaacct tcattccctg cgggaagaat ggctgtaatt ccgccagttt tgccttaatg
     7741 gctttcgcgg tatcgagagc attcgcgccg gttgccagct taatccccag gcccgccgcc
     7801 ggttttccgt tgatacgagc gataacgtta tagttttcac cgccaagttc aacccgtgcg
     7861 acatctttca ggcgtaccac cgagccgtca ctgtttacgc gcagggtcac tttgccgaat
     7921 tcttccggat tttttaaccg cgtttgagca ataatcgagg cattcaactg ttgccctggt
     7981 aacgctggcg ttccgcccaa ctgtccggca gcgatctgat cgttctgtac cttcaactgg
     8041 ttaatcacat caaccggtgt cagtttatat ttgtttagca gatcggcgtc cagccagata
     8101 cgcatcgcat actgtgcgcc gaaaagctgt acgtcaccga cgccattcag acggctaagc
     8161 gtatctttaa cgttagaggc cacatagtcc gagatatcgt cctgtgtggt gcctgggtta
     8221 tcagagacaa aacccgccac catcaaatag ctgctactgg acttttcaac actgatcccc
     8281 tgctgctgaa cctcctgcgg cagcaacggc gtggcgagct ggagtttgtt ctgcacctgc
     8341 acttgcgcga tatcaggatc ggtcccggac tggaaggtaa gggtaattgt cacgctaccg
     8401 gcggaatcgt tggtggagga catatacatc aggttatcga taccattcat attctgttcg
     8461 ataacctgcg tcaccgtatc ctgcacggtc tgcgcatcag cacctggata gtttgccgaa
     8521 acagaaaccg caggcggtgc aatggttgga tactgagcga cgggcaattg taggatcgcc
     8581 agtgcgcccg ccatcatcag aataatggcc agcacccatg caaatatcgg tcgtcgaata
     8641 aaaaagtttg ccatgtcaga ttaccttact tcgatgcagt atctgcgggg gtatcggtag
     8701 tggctttaac ctgctctccc ggacgcgctt tttgcaggcc gctgacaatg acttgatcgc
     8761 cagatttcag accttcgcta atcaaccatt tatcgccaat cgcctgactg gcaacgacag
     8821 ggcgcgcttc cacctgactt ttatcgttaa caatcagcac ggttgcatca ccacgcggtg
     8881 tgcggctaac gccttgttgc gggataagaa tggcgtcagg ttggacgcct tcatcaatcc
     8941 gtgcacgcac atacataccc ggcaaaagcg tatgttgcgg gttagggaag acagcacgta
     9001 gggttatgga gccggtgctt tcatcaacgg tcacatcaga gaattgcagc gtacctttca
     9061 ggggataggt ttgaccgttt tccatgacca actctacgtt gctggtggcg ttttccttat
     9121 gcaaatttcc ttgctctacg gattgcttca gcctcataaa atcgttgctg gattgggtca
     9181 catcaacgta gataggatcg agctgctgga cagtcgccag ttcagtcgtt tgcccattag
     9241 tgacaagagc gccttcggtc acggtcgatt tgccgatacg tccgctaatt ggcgcagtca
     9301 ctttggtata agcgagattg atgcgagcgc tttcgactgt gccttttgcg gcaatcacgg
     9361 cggcatcggc ctggcgagca tcagcaatgg cctggtcgta ctcctgctgg ctgatgtatt
     9421 tcgtacccac gagcggaacg taacgtttta ccgtcaaatg cgcgatggcg gcggcggctt
     9481 cacttt
//
LOCUS       DAJTNN010000163         9188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTNN010000163 DAJTNN010000000
VERSION     DAJTNN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.163"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(303..881)
                     /gene="mocA"
                     /locus_tag="OO150_003385"
     CDS             complement(303..881)
                     /gene="mocA"
                     /locus_tag="OO150_003385"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCR5517415.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITVQKRYTKI"
     gene            984..1754
                     /gene="yqeC"
                     /locus_tag="OO150_003386"
     CDS             984..1754
                     /gene="yqeC"
                     /locus_tag="OO150_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCR5517416.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKM
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1802..3427
                     /locus_tag="OO150_003387"
     CDS             1802..3427
                     /locus_tag="OO150_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCR5517417.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHINRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3468..4400)
                     /gene="arcC"
                     /locus_tag="OO150_003388"
     CDS             complement(3468..4400)
                     /gene="arcC"
                     /locus_tag="OO150_003388"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5517418.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNVLREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4448..5833)
                     /gene="hyuA"
                     /locus_tag="OO150_003389"
     CDS             complement(4448..5833)
                     /gene="hyuA"
                     /locus_tag="OO150_003389"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCR5517419.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5886..7097)
                     /locus_tag="OO150_003390"
     CDS             complement(5886..7097)
                     /locus_tag="OO150_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCR5517420.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7155..8351)
                     /gene="dpaL"
                     /locus_tag="OO150_003391"
     CDS             complement(7155..8351)
                     /gene="dpaL"
                     /locus_tag="OO150_003391"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCR5517421.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8409..>9188)
                     /gene="ygeW"
                     /locus_tag="OO150_003392"
     CDS             complement(8409..>9188)
                     /gene="ygeW"
                     /locus_tag="OO150_003392"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR5517422.1"
                     /translation="GAALDDGYKQGVLPQRPALVNLQCDIDHPTQSMADLAWLREHFG
                     SLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFGMDVTLAHPEGYDLIPDVVEV
                     AKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKVMEERTELLRANDHEGLKALE
                     KQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADISGVSCKEGEVTEGVFEKYRI
                     ATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQERVK"
BASE COUNT         2147 a         2245 c         2400 g         2396 t
ORIGIN      
        1 acagatatca ttgagtgacc ggtgttgaga aatccggtgg tttaggggga tagagcaaca
       61 atcgcaccac tcttgccagg tttggcggga aatataaaaa aatgtgaggc ggctcgaaac
      121 ttaagggctt tttgtgaatg aactcacagg tgctaaatga gaattaaaat cactcctgaa
      181 aattattatt gccagcccat atatcacttt ttctcttttc tcaaaatcag tgattcctta
      241 tcattatgat aaagagtgct ggagggatta aatatttgaa cgcaatcgtt taactacgtt
      301 ttttaaattt tagtatacct tttttgcact gtaataaagt cgtccggagt atcaatatct
      361 aaaattattt ctgcattttc tacttcgacg gaataatgag ctcccataag gagtgcctga
      421 cgcatattgg tgatattggg gcgcttgatt gcctgcatca ggcatggttt tgacactaaa
      481 atcggatggc cggggatgcc attatggagt ggcagtattg cgccatcatt tcgtaacgac
      541 cagatttttc tgaaaatatc gatggtgagg gttggcatgt caccgtgggt gagaaaacaa
      601 tgttctgttt gcactgcggg tactgcggcc tttactgatg ttagtaaacc ctgcgcataa
      661 tctgggttgt ggataatagt gatattgctc tggttcacat aacgttcgtg cagttcatta
      721 ccacgatagc cggtgactaa aataattcgg ctacaaaact gcaacgcatt tttgatactt
      781 gtatcaagaa ttgtcccctg ttgccagggt aacatcattt tccattgccc cattcttgat
      841 gataatccgg cagcggtaat tatacagtcg atggctgaca tagtggttcc attatttata
      901 ggggctaata acgcctgata attgcgttac ttcctgatgg attaagttgg ggataattta
      961 tcccagagag gtcataaaga ctcgtgaaaa gtatagttga cccatcggca ttattcattg
     1021 atttaggtgc gcagaaacgt cccactgtga tttctgttgt tggtgctggg gggaaaacca
     1081 gcttgctttt ttggctggca gaactgctcc aggctagcgg caggcgtgtg ttaatcacga
     1141 cgacaacaca tatgtttatg ccaacgtctc actggcccgt ggttttctgt cgtgatcccg
     1201 ccatgcttcc tcatgcgtct cttacatccc ccatttcatt ttgttttcac agctggaaag
     1261 cgaaccaggg aaaagtgcag ggatttacgc cagaagcgat tgatgcactg gtgcaacgac
     1321 cagagtgtga cgtaattctc attgaagcag atggctcgcg tgcaatgccg ttaaaagcgc
     1381 ctgatgagca cgaaccttgc atacccaaaa gcagttgctg cgtgattgct gtgatgggag
     1441 gacatatttt gggcgcgaaa atgagcacag aaaatgtcca tcgctggtcg cagtttgctg
     1501 atattactgg gttaacacct gatgcaacct tgcaactgag cgatctcgtt gcgctggttc
     1561 gccaccctca gggggcgttt aaaaacgtac cgcaaggttg tcggcgagtc tggttcatta
     1621 accgtttttc tcaatgtgag aatgcgattg cacaaagcga gctccttcaa ccgctgcaac
     1681 aacacgacgt agaggcaatc tggctaggcg atatacaaga acatcctgca atcgcgcgca
     1741 gatttgtgaa ttagcggccc gggtattccg ggttactgaa aggatatcca ttgaggttgg
     1801 tatgaatatt ttcacagagg ctgcaaaact cgaagagcaa aattgtccgt ttgcgatggc
     1861 gcaaattgtt gatagccgag gctcgactcc ccgccattct gcacaaatgt tagtgcgcgc
     1921 cgatggttct atcgtcggta caattggtgg cggaatggtt gagcggaagg tgattgaaga
     1981 gtcacttcag gcattgcagg aacgtaagcc gcgattattc catggacgta tggctcgtaa
     2041 cggtgcggat gctgtcgggt cagattgtgg aggtgcaatg tcagtattta tcagcgtcca
     2101 tggtatgcgt ccacgtctgg tgttgattgg cgcggggcat atcaaccggg cgatagccca
     2161 gagtgccgcg ctattaggat ttgatattgc cgttgccgat atttatcgcg aaagcctcaa
     2221 tcctgaacta ttcccaccat caaccacgct tctccatgct gagtcgtttg gtgcggcagt
     2281 ggaagcactg gatattcgcc ctgataattt tgtcctgatt gccacgaata atcaggatcg
     2341 tgaagccctc gacaaactca ttgaacagcc cattgcatgg ttggggttgc tggcaagtcg
     2401 ccgcaaggtt cagcttttcc tgcgtcaatt gcgtgagaaa ggcgtggctg aagaacatat
     2461 tgcccgttta catgcgcccg ttggttacaa cataggtgcg gaaacgccgc aggagatcgc
     2521 catcagcgtg ctggcagaaa tattacaggt gaaaaataac gcgccgggtg ggctgatgat
     2581 gaaaccttct catccttccg gacaccagct ggtggtgatt cgcggtgcgg gagatatcgc
     2641 cagtggtgtg gcgctacgtc tgtatcatgc gggttttaaa gtgatcatgc tggaagtgga
     2701 aaaaccgaca gtgattcgtt gtaccgtggc gtttgcccag gccgtgttcg atggcgaaat
     2761 gacggtcgaa ggcgtcactg ctcgcctggc aaccagctct gcggaagcga tgaaacttac
     2821 cgaacgcgga ttcatccctg tgatggtaga tcccgcctgt tcattgcttg atgaactgaa
     2881 accgctttgc gtggtggacg ctattctggc gaaacagaat ttgggaacgc gggcagatat
     2941 ggcaccagta acaatcgcgc ttgggccggg ctttactgca gggaaggatt gtcatgcggt
     3001 aattgaaaca aatcgcgggc actggctcgg tcaggtgatt tactctggtt gtgcgcagga
     3061 gaataccggt gttcctggca atattatggg gcataccacc cgacgggtga tccgtgctcc
     3121 tgctgcaggc attatgcgat ccaacgtgaa attaggcgat ctggtgaaag agggcgatgt
     3181 gattgcctgg attggtgagc atgaaattaa agcaccgttg acggggatgg tgcgtggctt
     3241 gttgaacgac ggcctggcag tggttggtgg ttttaaaatt ggtgatatcg atcctcgtgg
     3301 tgaaaccgct gatttcacca gcgtttctga taaagcccgg gcgattggcg gcggcgtact
     3361 tgaggcgtta atgatgttga tgcatcaggg cgtgaaagcg acaaaagaag tgctggaagt
     3421 ggcttaaatg aaaatggccg gatggtatta cccacccggc cttaaaatta ctgacaaatc
     3481 accgttccgg ttttgccttc aatcccttct ttcgctttgc tcaatacggt aatcagtgct
     3541 tcacgacctg cgcgagagcg agcaaacgag gcggcagctt ccactttagg cagcatagaa
     3601 cctttcgcaa aatgaccttc ttcgataaag cgttcagcat cacttaatga caggcgatcg
     3661 agccactgtt cattctcttt accgaagtta atggccactt tttctaccgc agtcagaatg
     3721 atcagcatat cggcatcaat catttctgct aaacgggcgc tggcccagtc tttatcgata
     3781 accgcgctgg caccacgcag atggttacct tcacgaataa cagggatacc accaccgcca
     3841 acggtgatca ccacctggcc tgcatctacc agagctttaa cagtttcttt ttcaatgata
     3901 tcaaccggtt ttggcgaggc aacaacacgg cgatagccgc gaccagcatc ttctttcaga
     3961 gtgtaaccct gttttgtcag ttgctctgct tcttgctcgg taaagaacga accgatcggc
     4021 ttggtcgggt tgaggaatgc cggatcgtta gcgtcgactt cgacctgagt aaccagcgtt
     4081 gctacaggtt tattaatgcc ccgagaaagc agttcttctc ttaacacgtt ttgtagatcg
     4141 taaccaatat aaccctggct taaggcaaca cagacagaca tcggcagcat tggcgagtgt
     4201 gcttcagttt ttgcggcagc ttcaaaggcc tggttaatca tgcccacctg cggcccgtta
     4261 ccatgagtga cgataacttc atgtccttgg gcaattaaat caacaattgc ctgagaagta
     4321 atttttaccg ctttcatttg tccggccagg tcatcgccca gcgcattccc gcccagggcg
     4381 agaacaattt tcttactcat ttttcctcac tcatcattct tgattgtgta tagatccctg
     4441 acagtggtta gagcacggga gggacaaacg gtttgcgtcg caggaaacga cctcgcccgg
     4501 ctttgcctgt aaaggtgccg tcacagaaaa tcgtttcacc acgggataag gttctgacaa
     4561 tcgcgccctg acaggtaaaa ccctcccatg gcgagtagtc ggcgttgtcg tggagatggc
     4621 gatgctgaat ttgttggctc tgacgtgggt cgataatcac cacgtcgcca tcggaaccgg
     4681 gcgctaataa tcctttttgc ggccacaggc caaacagcct ggcgggcatt gcgctggtta
     4741 attcaacaaa gcgttccggt gttatacgtc ccgtcatcac gccactggaa aacagtaact
     4801 gcatgcggtt ttccacaccg ggtaagccat ttgggcagcg actgaaatcg cctttagaaa
     4861 tttgcaggcg ttgagccatc gagaaggtgc agtgatcggt cgccaccacg tcaatcgcac
     4921 catcgctgat gccacaccac agtttgtcct gctcgcgtac gttacgcagc ggtgggctaa
     4981 gaatgaactt catgccatct tctgtatcgt aactgcgttc gtccaacagg agatattgtg
     5041 ggcaggtttc aacccagact ggctggtgat tcgcacgggc aagacgcaga taatccagac
     5101 ctaagccgtt agacaggtgc acgatataga gcggggcgtt accggcaatt tgtgccaggt
     5161 taatcatgcg ggcgatggct tccgcttcgc attccagagg gcgactcaag gcatgatagc
     5221 gcggcgcggt taaccctgcg gcgataaatt ccgcccgctt gctggcgata gccgcatcat
     5281 tttccgggtg cacggtggtc agcgcgccgg attcatgcaa gcggcgtaat gcctgcaaaa
     5341 cctcgtcatc gttgagtttg tattgatagg ttaaatagag tttaaaactg ctcagtcctt
     5401 cctcgaccat catcgggatt tcgtcgagga ttgcgtgatt aatgtgctgg atcacaccgt
     5461 gaaagctgta atcgatgacc gctttatggg cggcataacc acgataaacc tccagttgat
     5521 ggcgtaaccg acagccgttt gggccaaatc ccatatggtc aataatggtt gttgtaccgc
     5581 cacacgcagc tgcgcgggta ccggtaaaaa aatcatcaca actgcgcgcg atgccgacat
     5641 caatattgaa atgcgtatgg acatccacgc cgcctgggaa aacgtaacag ccagtggcat
     5701 caatttcttc atacgggagc tgcggcgaaa tattgttgcc caactggcga acaatcccgc
     5761 tttcaatcag caaatcctgt ttggcttgtc catctgcgtt aacgacagtg ccgtttttga
     5821 tcaatacgcg catagcaaac tccaaaaacc ctccggcgaa ccggagggga ataagtgaag
     5881 agtaattact ctgttgccag ccagcttaac gggattgcag cgtacatcgc ggcacaggtc
     5941 accaggtgag atttccaggt tttttcgtta ggtgcatgcg cttcaggttc tttacccggg
     6001 ccaaagccga tcaccggaat gccgtgacgg cccatgatag atacgccgtt agttgagaag
     6061 gtccacttat caacaaccgg cgctttgcca aacagccctt cgtaggcatt caccagtgct
     6121 ttaacggtga agtgatcttc ttccactttc caggtcggga agtagcattc ggttgggtaa
     6181 accaggccag tccaggacgg acggtcgtag ttgtacatag aaacaaccgc gttagctttc
     6241 tgtactgcag gcagggcgcg gatttcgtcc agcgcgcctt cccaggtttc gccccaggtc
     6301 agacggcggt caatagagac ggcgcagcta tctgctacag cgcaacggct tggggatgtg
     6361 aagaagattt cagaaacggt gagggtgcct ttgccgagga attcgtcata acccagacgt
     6421 tgggagagtt cttgtaattc gccaagaatc ggacccattt tgaaaatggc gttgtcaccg
     6481 cgttctggcg cagaaccgtg gcagctaaca ccctgaacat caatacgaat ttccatacga
     6541 ccgcgctgac cacggtatat ctggcagtcg gttggttcgg tactgaccac aaattccggg
     6601 cgaatgccgg attgttcaat aatgtactgc cagcacagac cgtcgcagtc ttcttcctgc
     6661 acagtaccgg taaccagcag ggtatattca tcttccagac cgaggtcttt aatgatttta
     6721 ccggcataaa ccatagatgc catgccgcct tcctggtcgg aagtaccgcg accaccgatt
     6781 agctcatcag tttccatgcc ttcgtacgga tcgaagtccc agtttttgat gttgccaatg
     6841 ccgacggtat cgatatgagc gtccattgcc accagacgcg ggccgtggcc gatataaccg
     6901 agaacgttgc ccatcgggtc gatttcaact ttatcgaagc cgactttttc catctcttct
     6961 ttaatacgat gtactacgcg tttttcgtcg cagctttcac tgggaatagc aaccatgtcg
     7021 cgcaggaagc gagtcatgtc cgcctggtaa tcttttgctt tttcaagaat cagtttgaat
     7081 ggaatattct tagccattat ttttctccag attttttact ccccgttttg cagggagtag
     7141 ttccaatagg gtgattaagg tgctacagcg tgtttgcctt cccagacaac ttcgcggtag
     7201 tgcttcacgt cggtgtcgcc ttcagtactg ataaccagca ctacggcatc tttgttcagc
     7261 gccagttttt ccatcaggct ttgacgttgc gggtgataat gaaccgctgc gagaacgccc
     7321 aaaccgacag cgccggattc accggagatg atgcgcgggt cgttgccgta cggattaccc
     7381 agcacgcgca tacctaatgc ggcaacgctg tcctggcagg agatgaattg ggtggcgcag
     7441 ttacgtagga tttcccagcc cagcgggtta ggttcgccac aggccaggcc cgccatgatg
     7501 gtggccatat caccgccaac gttgacgatg tcgcctttga cgccggagcg ataaatacag
     7561 tcagctttgt caggttcaac aataatgctg tgcagatttt gcgggctgta gacgtcgacc
     7621 agataaccca gcacaccacc ggccattgct ccgacaccgg cttgcagcag aacgtgcgtc
     7681 ggggttacgc ccatttcacg catttgctcg acggcttcat ctgccagggt tgcgtagcct
     7741 tgcatgatcc aggttgggat tttggtgtaa ccttcccatg ccgtgtcctg taccacttcc
     7801 cagccgtgct gctgcgcgtg ttgcatggtc aggcgaacgg tatcgtcata gttcatatcc
     7861 gtgacgatgc actcggcacc gaggttcaga atggcgtcaa cgcgttcctg agcagaacct
     7921 ttcggcatgt aaatcaccgc attctgtccg agttgctgtg ctgcccacgc cacaccgcgc
     7981 ccgtggttgc cgtcggtggt ggtcgcgaaa gtcatttttt cgccgatggc atttttcagg
     8041 tgctcaaatg acagcgtttc gatatcaaga tgatattttt cacacaataa ttgagcgatg
     8101 gcgtacgcac cgccaagcat tttgaaggcg ttcagaccga atcgttttga ttcgtctttg
     8161 acgagaattt tcttcacacc aaaaaggttt gcgagatcgt ccagcgcaca aagcggtgtt
     8221 gggcgataac cagctatttt ctggtggaac tggcgcgcca gtttggcctg gctttgcgaa
     8281 aagagcggtg atgtttcgcc gttgaaaaat ttgttatcgg cgatatcaat cttcaatgag
     8341 aaaacggaca tatcctatcc ttttggcctc tggaaataga gggccgacca gctgatcggc
     8401 ccgaggggtt atttcacgcg ttcttgcgct tctttcagca gttgctcgag cagtgcacca
     8461 ggtttggcgt atttacggga caggatcatc gcggcgatga tataaggctt ccagctggct
     8521 tctttgtagg tagcgatacg gtatttttcg aatacgcctt cagtcacttc accttctttg
     8581 caggatacgc cgctgatatc agctggcagg cagtgcatgt acagggcttc gccatcacgg
     8641 gtcagttcca tcatctcttc agtacaatgc cagtctttgt gttgcgcgtt ctgtgccaga
     8701 cactgttttt ccagtgcttt taagccttcg tggtcgttcg cacgcagcaa ttcagtacgc
     8761 tcttccatca ctttgtaagg tgcccatgac ttcggataaa cgatgtctgc gtctttgaag
     8821 gcttcttcca tgctggtgac ctgacggaag ctaccaccgg aggctttagc attgtttttc
     8881 gccacttcaa ccacatccgg gatcaggtcg tagccttccg gatgggccag ggtgacatcc
     8941 ataccgaagc gagtcatcag accgatgatg ccttgtggta cagagagcgg tttgccatag
     9001 cttggagagt aggcccaggt catggcgatt tttttacctt tcaggttttc cagtgaacca
     9061 aagtgttcac gtaaccacgc gaggtcagcc attgactgag tcgggtggtc aatatcgcat
     9121 tgcaggttca ctaaagccgg acgctgtggc agtacaccct gcttgtaacc gtcatcaagt
     9181 gcagcgcc
//
LOCUS       DAJTNN010000164         9039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTNN010000164 DAJTNN010000000
VERSION     DAJTNN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.164"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..67)
                     /locus_tag="OO150_003393"
     CDS             complement(<1..67)
                     /locus_tag="OO150_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5517423.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMR"
     gene            75..506
                     /gene="tnpA"
                     /locus_tag="OO150_003394"
                     /pseudo
     CDS             75..506
                     /gene="tnpA"
                     /locus_tag="OO150_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(637..1960)
                     /locus_tag="OO150_003395"
                     /pseudo
     CDS             complement(637..1960)
                     /locus_tag="OO150_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1960..3576)
                     /locus_tag="OO150_003396"
     CDS             complement(1960..3576)
                     /locus_tag="OO150_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5517424.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMIWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGGWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3872..4588
                     /locus_tag="OO150_003397"
     CDS             3872..4588
                     /locus_tag="OO150_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5517425.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4585..6246)
                     /locus_tag="OO150_003398"
     CDS             complement(4585..6246)
                     /locus_tag="OO150_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5517426.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLFWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAPALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6442..6870)
                     /gene="ibpB"
                     /locus_tag="OO150_003399"
     CDS             complement(6442..6870)
                     /gene="ibpB"
                     /locus_tag="OO150_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5517427.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6982..7395)
                     /gene="ibpA"
                     /locus_tag="OO150_003400"
     CDS             complement(6982..7395)
                     /gene="ibpA"
                     /locus_tag="OO150_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5517428.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7389..7461)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7701..8033
                     /locus_tag="OO150_003401"
     CDS             7701..8033
                     /locus_tag="OO150_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5517429.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSHVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8035..>9039)
                     /locus_tag="OO150_003402"
     CDS             complement(8035..>9039)
                     /locus_tag="OO150_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5517430.1"
                     /translation="PKSEKYVFIHGPENPDETWYYDFHHRRGVIAEGGKVSNLDAMDI
                     TAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNVGVAAPFGPVNVQ
                     KQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAFIGDTLSPKGEKV
                     PELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTHHRAYPGLVNVPR
                     HWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQSAFNWHPSGEWL
                     GFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWMEGGQLWITETDR
                     "
BASE COUNT         2302 a         2378 c         2257 g         2102 t
ORIGIN      
        1 gcctcatttc acgctcctgt tgaccaccgg gtcttaactg gaatttaaat gcttgtagtc
       61 gtttcacagg ccaaatgata ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa
      121 ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac cgacgtcaga
      181 tttttgacta tgacgcgaca gaaaaactac gcacttactt ttcaaatgta tgtgctgatt
      241 ttgaagctga actggtttaa atggatggcg aaccagatca cgtccatttg ttaattaact
      301 atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta tcgggtaggt
      361 tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc gttttgtgga
      421 gtcctggcta ttttgccaat agctgcggag gtgcgccaat atccgtcatc cgccaataca
      481 ttgaacaaca gcaaacacct ggttaggtgg aaaaccgcgc cttatatccc cgccctgaag
      541 gacggggttt tacggcgcac cggataaatt tggttatgcg ccgtcagccg tgctaaagaa
      601 tacggaccag cacccaacgg atgccagtcc acacaattaa cgtaactctg gccagtacgc
      661 tttgttggcc tccagcaact catccagaat agctttggcg accgaggcgc tcggtaccgt
      721 tttcgataac gtaatcgcct gccacaggtg ctgatatgaa cgctgttccc aggcgtccac
      781 gaccagtttt tccaccgcca cttgctgact cattaaccct ttctgaaact gcgggatatc
      841 gccgaccacc agtggttctg gtccgttgtg cccgaccaga cacgggatct cgaccatcgc
      901 ttcatcatca aagttatgga ttgccccgtt gttaggcaca atcagcaaca tccgctcctg
      961 agtgttgaag gcaattgccg ccgccagatc gacgatgtat gacgcatgtt cgtcaatctc
     1021 cagcttggcc gccgctgaat ttcctgccgc tgtaatggcg cggcaagcat cgaaaacctg
     1081 tttctcgcga tgttccattc acctcattcg cgcgggtatg ttcagggttg gaatgctgaa
     1141 caacataatc cgggaagaga taatatttca gataggtgtt cggcaatgta tccgggtcca
     1201 gtgcctggac atcccgcgct tttgcatagg tgtcgttcca gctcgcttca gtatgtgtat
     1261 cttgctgcgg tggaacataa ccgtgcttag agacatattg ccgcagctgg ggcatcaggt
     1321 cgttgccctg caaatcttca attgatgtcc accagccaaa gtggttcagg ccgtagtagc
     1381 gcacgcgcat ctgtttgcga tcttgcagcc caacaatttg cgccatccgg ctttcaatac
     1441 cgattggcat gtcacagatg ttgaggattt tcgcattcgg gcgcagacga cgcgtggctt
     1501 ctgcgacaat tgctgccggg ttggagtagt tgagcatcca ggcatttggt gaatattttt
     1561 ccatataatc caccagttcc aggacgccgc caatggaacg catgccgtac gctattccgc
     1621 cgggtccgca agtttcctga ccaacaacgc cgtggcgcag cgggattttt tcatccagtt
     1681 cgcgcatcgg gtatttgccg acgcggatgt gcgccatgac aaaatcaacg tcgctgaatg
     1741 ccacttcagg atcggtggtg taactaaacg caatgtccgg cgctttctct ttaaggatga
     1801 ctttacaggc ttcggcaatc acttcctgcc gcgcaccatc gttatcataa aatttcagtg
     1861 cacgaagcgg gaaacggtcc tgattcgcca ggagcatcaa cacgatgcct ggcgtaaagg
     1921 tgcttccacc gcctgcgaca accactgaga atttggtcat tatactgcct ccgtaatggc
     1981 aacattttct gctgattgat gagaattaat taagctatcg agctgttcac gcagctggga
     2041 tacatgcaga ccaatgatca cctgaatggc atcgccacta cggaagacgc cgtgcgctcc
     2101 cagcttttta aagacttcgt catccagcgt ttgtgacatg tcatgcagtg caatacgtaa
     2161 acgcgtcgcg caattgttaa tgctggagat attgccgacc ccgcccaggg cttgcaggat
     2221 accggcagcc tgatccagct ctttttttgg ctctgcagcg gtggtttggc ctcgcgaggc
     2281 tttgtattcg gcttttgagt agagtttcac ttccgcatct tcacgtcccg gcgtgcacat
     2341 gttgaattgc agaatcagtg tgcggaaaac cacgaagtac agcagggtaa agcacaaccc
     2401 aatggcgatt tgggtcagca tcatatccgc gtggttgctg aacatcggga tccagttttg
     2461 cggtaaaacc tggtcaatca gacctccgcc catgttgccc accacaccaa agagatacat
     2521 cacggtcgac attgaggccg ccagcacggc gtgtaccgca aacagcaacg gtgaaatgaa
     2581 caggaaggta aattccagcg gttcggtaat tcccaccagc atggcggtta aggttgcggg
     2641 aatcagcaag cccgccactt ttacccgatt ttccggtgcg gcagtgaagt acatcgctaa
     2701 agaaatgccc acggcaccaa agatttttga gttaccgtgc agggcaaaac cgccttccgg
     2761 gaacaacgat ttcagcggct cggcgctcag actgaactct tgcagatgct gcgcccagta
     2821 catctgaatg ccgccttcaa cagctgccgg accaaagata aactgtccgt agatgaagtg
     2881 gtgtaaaccg gttgggatca gaatacgttc gaggaaggtg taaacccacc ccccaagcgc
     2941 acccgccgaa cgcaggaacg cttgcagaga ttcaatcccc atttgtactt ttggccaacc
     3001 gagcaacgtc agccaggcac aggggatcat caccaggaag gcgataatca ccacataaga
     3061 cgtcccctgg aaaatgccga gaaaaaccgg cagtttttta tcgaacagac ggttatgcag
     3121 cgccgtcaca atgccggaaa tgataattgc gccgataatg ctggtatcga gggttttaat
     3181 cccggccatc attgtcagac cgctacctgc caccgcgtcc tgagtgaaat cgacgccgaa
     3241 gtagcttccc cagatcattc ccatcgcgtt gatgaaatag ttccaggtca ggaaactcac
     3301 catcaccgcc agacaagcac gcccctgcgc ttgcttagca aggccaatgg gtaaaccgac
     3361 agcaaaaatc agcggcatat tacggaatac cgtccaaccg ccctcttcaa taatgtgtac
     3421 gatttgcgcg aataaactgt tcggatcggt cagtgattcc ccgacaaaca tcgggttttg
     3481 cagcaagatg gcaagaccca ccacaatccc ggcgaaggga aacagcagca ctggcgtgaa
     3541 catcgcgccg ccaaagcgtt gaatttgact gagcatttcc acattctctt ttctcaattc
     3601 tgtaagtagg aagcctgatt ggttatgcgg caggcctgga gcgaggatag gaagatgcga
     3661 tcggcagaac atgacgaccc aatgggattc gtgatcgctt tcatgctttt cgttgggcgg
     3721 tagagatcta cgttttcgcg aaaaagtaga catctgtgag cactggtaca cagcatcgaa
     3781 cacatcttta aaaaaaagat gttttttcaa tcgattaagc agaacttgtg ggcgcattac
     3841 ccgggcttgc aggcaaaaaa gagatctaga gatgatctac aaaagcattg cggagcggtt
     3901 aagaattcga cttaattccg cggatttcac gctaaacagc cttcttcccg gtgaaaaaaa
     3961 gttggcggaa gaatttgcag tatcccggat gaccatccgt aaagcgattg acctgctggt
     4021 agcgtggggg ctggtggtcc gccgccacgg cagcggcact tacctggtgc gcaaagatgt
     4081 gctgcatcaa accgccagcc tgaccggact ggtggaggtg ttaaaacggc agggaaaaac
     4141 ggtcaccagc caggtgctga tttttgaaat catgcctgcg cctccggcca ttgccagcca
     4201 gttacggatt caaatcaacg agcagatcta cttctcccgt cgcgttcgtt ttgtggaagg
     4261 gaaaccgctg atgctggaag acagctatat gccggttaaa ctgttccgta atctttcgct
     4321 gcaacatctg gaagggtcga agtttgaata tattgaacaa gagtgtggga ttttgattgg
     4381 cggtaattat gaaagcctga cgccggtgct cgccgataga ctgctggcgc ggcaaatgaa
     4441 ggtagcggaa cacacgccac tgctgcggat cacctcgttg tcatatagcg agagcgggga
     4501 gtttttgaat tattcagtga tgttcagaaa tgccagcgaa taccaggtgg agtaccattt
     4561 acggcgactc cacccggaaa agagttaacc gatactccag aagagcaccg ccagtaattg
     4621 gggggtgata attcgcagaa acatcactaa cggatagaca gtggcgtaag agagcgccgg
     4681 cgcaccactg gttggatgaa gattattagc aaacgccagc gccggaggat cggtcatgga
     4741 acctgccagc atcccgcaca tggtcaggta attcattttg gctaacatcc gcgccagaat
     4801 gccaacagta atcagcggaa cggcggtgat cagggcacca taaccaatcc agcttagccc
     4861 ttcgccattg accagggtat tcacaaaatc cccaccagat ttcagaccaa cgaccgagag
     4921 gaacagcacg atccccagct cccgcagcgc gaggttggca cttggcggca taaaccagaa
     4981 cagcttgccg atactgccga tacgcccgag gatcaacgcc ataatcagcg gaccgcccgc
     5041 cagccccagt ttcaacgcgg ccgggaaccc aggcacaaag acgggaatag aacctaacaa
     5101 tacgccaagc ccgatgccaa taaacactgg cagcatctga acctgttgca gtttttgctg
     5161 cgcattcccc agcacattgg caacggcatc aattgcggac ggacgcccca ccagattgag
     5221 gatatcgccg aactgcaggc tgatatcgcc actggcgacc agttcgaccc cggcacggtt
     5281 caggcgcgag ataacaacgt catagcgttc tttaaagtgc aggtcgcgaa tacgttttcc
     5341 gagcacgttt tcattggtga ccaccacacg ctcgacgcgc aaatcagtgc ctttcgtgga
     5401 cagcgaagta tcgacctcct gaccaatcac cagttgcgca ttatgtaaat ccgctggctg
     5461 ccccaccaga tgcagcaaat cgcccagttg gataatggta tctggcgaag gaacttttag
     5521 ggtttcttcg cgtttcagac gcgagcagat aattttgtcg ccgttgagaa tcggtacatc
     5581 tttaatagct aaatcatgca ggttagggtt ctcaacgcga atattgatag tcttgatcag
     5641 cgcgccgcca ttggtgcgtg aagactcgtg ctgctgagct tctgtctcga cattgacgcg
     5701 gaaaataacc cgcaacatcc acatggtgaa caaaatcccg caaatgccga atggatacgc
     5761 catcgcgtaa ctcatcccca tctgatcgac catttccatt ggtgtaccca ggtcgcgcaa
     5821 gatctgctgc cctgccccca gcgctggcgt attggtaacc gcaccggaga aaatccccag
     5881 cactaccggc agtggaatat caaacagttt atgcaggatg gcggtaacca gaccaccgat
     5941 gatgacgatc agaacagcaa acaggttgag gcgtaatccg gagacgcgca atgaggcaaa
     6001 gaagcccggc cctacttgaa tcccgatggt ataaacgaac aggatcaggc caaattcctg
     6061 aataacatgc agcatatcgc tacttaatgt catccccgcc tgagaaacaa aatggccgac
     6121 gatgatccca ccaaacagca cgccgccaat acctaatcct atgccgcgaa atttgacgtt
     6181 gccgataaac aaaccgacga ctgccaccaa agccagaata ctgaccgtta atgctatatc
     6241 actcatcgtc catttctctt gagataacat tttaattgct acggattatg gctgaatcgg
     6301 tgaagcagat ttgccttata gcgcacatta tcacgggaaa gaacgtgccg aaatatctta
     6361 aacagtcgaa atttaacgca ttgttgaaga agaaagacaa aaaaagcccc accagaatgg
     6421 cggggcaaag agaatagcta gttagctatt taacgcggga cgttcgctga tagcgatacg
     6481 ctgcgctgcg atgggttcag gctcattacg aattaaatca atatgcagta aaccgtttac
     6541 gaaggttgcg ccagagactt ccatattttc agccagcgta aagctcaggc taaatggctg
     6601 attcataagc ccttgatgca gccatttttt ctcttctttt ggctgctccg gcgtgccttt
     6661 tacgctcagg cgcgtacctt ccagttgaat ctctaaatct tcctgacgga aacctgccag
     6721 cgcaagggta atgcggtagt ggttatcgtc gcttttctca atgttgtacg gcgggaagct
     6781 ctggctttca ccggcgtttt gcagtgcgtt ggccagtttg tcaaaaccga tccattgacg
     6841 catcagtggg gataaatcga agttacgcat agtcatttct ccttctaaga agcgagtaag
     6901 tacctgcaaa tccgaagatt cgcatatgct ccctgacggc gagcatggag atgtcaggcc
     6961 gcgtcaggcg gccttaggga attagttgat ttcgatacgg cgcggttttt tcgcttccgg
     7021 aatcacgcgt tcgagatcga tatacagcaa accatttacc aggttagccc cacgaacatg
     7081 aatgttctca gctaactgga atttgcgttc aaagttgcgt tcagcgatgc cctggtacag
     7141 ataggtgcgc tctttttgtt cgtcggcgtg agcacctttc accaccagca gattatcctg
     7201 ggcggtaatt tccagttcgc tctcagcaaa accagccaca gcgatagcaa tgcggtaatg
     7261 gttttcgtct accagttcaa cgttatacgg agggtagccg ccattactct ggctctggtt
     7321 gttttctaag tggttaaaca aacggtcaaa tccaatagca gaacggtaaa gcggggataa
     7381 atcaaagtta cgcataatca atagctcctg aaatcagcga gaatgtaata ccttccacaa
     7441 tggacaggtc aggtagccag aacacccatc aggcgcgtcc tcatcggcta cgatgtaaaa
     7501 atgggtctgg aaatgaactt ttcaagcctt atcagcggac ttttttttgc agtttatggt
     7561 ctattgcata gactgagggg gcagcacgaa acgttaaagt gcgatagccg ccacagtgcg
     7621 acgtaatgat gatgttattt tcagcaaggg atcgctataa ctcatcaggc caacacccac
     7681 gatcaacaga tgaatttatt atgataagaa atgtgttgtt agcgttcatg atatgcagcg
     7741 gaatgacatt actcggagga tgctccagcg tgatgtccca taccggaggg aaagaaggaa
     7801 cgtatcccgg cacccgcgcc agcgcaacca tgattggcga cgatgaaacc aactggggca
     7861 ctaagtcgct ggcgattctc gatatgccgt ttaccgctgt gatggacacg cttttactgc
     7921 cgtgggatgt gttccgcaaa gacagctcgg tgcgctcaca cgttgaaaaa agcgaagcca
     7981 acgcgcaagc caccaatgcg gttattccac cggccagaat gcctgataac tgatttagcg
     8041 atcagtttcg gtgatccaca gctggccgcc ttccatccac gccagccatt gaccatccgg
     8101 cgagaagacc acggcgtccg cagaaggtgg attggcgtgg ttttcggtta aatactcaac
     8161 ctcgccactt tgcgcatggg cacaagcaat tcgattatcc agcacaaagc ccaaccattc
     8221 tcctgacgga tgccagttaa atgcagactg aatatccgtt ttgttatggg ttaactggcg
     8281 cggctcgccg ccctgtggcg agataagcca cagttgcaca atgccgttat catcacgcat
     8341 taaaaacgcg atttgcgtac cctgcggatt acagcgcacc cagtggcgcg ggacgttgac
     8401 taaccccgga taagcccgat ggtgggtaaa ggttaaacgt cgctgcacga cgccgcgcgg
     8461 tggcgcgggc agggtggttt ccgttccact taacggcgca tcacctgccg ctttccagcc
     8521 agcttcatct tgcggtaact caacgataaa cagctccggc actttctcgc cctttggcga
     8581 aagtgtgtcg ccaataaacg ccagcgcgtg atttcctacc catccttctt cataagcacg
     8641 attgatttca tcgctgccag gctgcggcgt gggcgtggtt ttactcacca gcacgcacca
     8701 gtggctaccg ctgtattcac gcggatgctg cttttgtacg ttgaccgggc caaacggcgc
     8761 agcaacaccg acgtttcgca aatccagcgc cggatcgagt tcatgcatta catggtcgtt
     8821 ataggtaaag ctcacccttt caccgttcgg gctaaagaca tgcacatggc tgccgccgcg
     8881 cagcgctcct ggcgtatacg gcgcggtaat atccattgca tcgagattgc tcaccttgcc
     8941 gccttcagca atgactccgc gtcgatgatg gaaatcgtaa taccatgttt catcaggatt
     9001 ttccgggcca tggatgaaaa catatttctc tgacttcgg
//
LOCUS       DAJTNN010000165         8833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTNN010000165 DAJTNN010000000
VERSION     DAJTNN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.165"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..680
                     /locus_tag="OO150_003403"
     CDS             <1..680
                     /locus_tag="OO150_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5517431.1"
                     /translation="GVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLPWTLKSHHEDAD
                     GVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETTSALHTYFNVGD
                     IAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCSVINDEALNRII
                     AVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQKVTKEKPAHLAQ
                     SIRVAKR"
     gene            complement(731..1585)
                     /gene="yeaE"
                     /locus_tag="OO150_003404"
     CDS             complement(731..1585)
                     /gene="yeaE"
                     /locus_tag="OO150_003404"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR5517432.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1675..2421)
                     /gene="mipA"
                     /locus_tag="OO150_003405"
     CDS             complement(1675..2421)
                     /gene="mipA"
                     /locus_tag="OO150_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5517433.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2857..4791
                     /gene="yeaG"
                     /locus_tag="OO150_003406"
     CDS             2857..4791
                     /gene="yeaG"
                     /locus_tag="OO150_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5517434.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4904..6187
                     /locus_tag="OO150_003407"
     CDS             4904..6187
                     /locus_tag="OO150_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR5517435.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6334..7809
                     /locus_tag="OO150_003408"
     CDS             6334..7809
                     /locus_tag="OO150_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5517436.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPSQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATRGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIADRIRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            7903..7989
                     /locus_tag="OO150_003409"
                     /pseudo
     CDS             7903..7989
                     /locus_tag="OO150_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            7990..>8833
                     /locus_tag="OO150_003410"
     CDS             7990..>8833
                     /locus_tag="OO150_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5517437.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGREVAK
                     FSVPL"
BASE COUNT         2327 a         2116 c         2054 g         2336 t
ORIGIN      
        1 atggcgtcgc tattcgcggt ggcgtaccgg tttgctggcc gtggtttggt ccggcggcac
       61 aacaaggtct gcctgcgcac ggttttgccc gcaacctgcc gtggacgctg aaatcacatc
      121 atgaagatgc tgatggcgta gcgctgactt ttgaattgac gcaaagcgaa gagacgaaaa
      181 aattctgtcc gcacgacttt acgctgttag cgcatttccg cgtgggtaaa acctgtgaaa
      241 tcgatcttga atcacatggc gaatttgaaa ccacctctgc cctgcatacc tactttaacg
      301 tgggtgatat cgctaaggta agcgtcagtg ggctgggcga tcgcttcatt gataaagtga
      361 atgacgcgaa agaagatgta ctgaccgacg gtattcagac cttccctgac cgtaccgatc
      421 gcgtgtatct gaatccgcaa gattgcagcg tgattaatga tgaagcgctg aatcgtatta
      481 tcgccgtagg ccaccagcat catctgaacg ttgtaggctg gaacccggga ccggcgcttt
      541 caattagcat gggcgatatg ccggatgatg gctacaaaac atttgtttgt gtagaaacgg
      601 cttacgcttc agaaacgcaa aaagtcacca aagagaaacc tgcacatctg gcgcaatcca
      661 ttcgcgttgc gaaacgttaa tttgcgttaa tgttgtgtgc cgggtgcgat gcatccggca
      721 cgtatcaaca tcacaccata tccagcgcag tttttccttt tggtgccgga tacgctttat
      781 ccagcatggt taattccgct gacgaaagtt cgacctcaag cacagccgca ttctgttgaa
      841 catgggcaat cgtggccgct tttggaatcg ccatcacacc ctgatggctg attaccctcg
      901 ccaacaatac ttgtgccgag ctgatattgt gagcatgtgc aatttcgttg actaccgcgt
      961 tttttaacag tccattgcgc aaccgcccgg cctgggctaa tggactgtaa gccatcaccg
     1021 gcatctgctg ttgctggcac caggggagta gatcgtactc aatccctcgt gaaccgagat
     1081 ggtaaagcac ctgattagtg gcacactgat ttccccccgg cagctgccag agttcctgca
     1141 tatcagcata atcaaggtta gaaacgcccc agcggcggat ttttccctgg gcgatcaatt
     1201 tttccatcgc tgcaacagtc tcttcaaaag cgaaactgcc agaccagtgt aataagtaga
     1261 gatcgagata atcagtattg agacggcgta aactggcttc gcatgcattt atcgcttttt
     1321 gcctgccagc attccacgga tagactttag agacgagaaa gacgttatca cgcagaccgg
     1381 ttaatgcttc cccaaccacc ttttcggcac cgccatcggc atacatttcg gcggtatcaa
     1441 tgagggttaa accgagctca atgcccgcgc gtagtgcagc aacttctgtt ttgcgctgac
     1501 tggcatcttc gcccatatac catgttccct gccctatggc tggcagtgag acatcgccac
     1561 taaattgaat cattttttgt tgcattgttt cctcccaggt attgcaccac cgtaatgcaa
     1621 aacagggcgt gacgccctgt ttttatgcac aaaatgccct ggaaagatgc attatcagaa
     1681 tttgtaggtg atcccggtag aaatcaggcc agtccaggat ttatccacca tcgggctgtc
     1741 agtaacttca tcagacagac gggtatagcg cgcggtaccg taaacactcc agtcgccgag
     1801 gaagttgtag ctggcgctca gctccaggta agggctccag ctgtcgttcg ggttatagcc
     1861 acgcagaccg ctgcgagcgg actctttgcg cgatacgcca taatagtatt cgttctggtt
     1921 ttcgctgttc cactgcacac caatacccgg agtcacggtc aggccaccgt tggtgtaacg
     1981 atacaaccag gccatatccc agacaatgcc gttgctgtta tccagggtat cgccagccag
     2041 ggtggtacgc aggtaaccgt actgggtaaa gtgagcataa gacagaccag ccatcatggt
     2101 gctcttacgg tcatccaggt gacgcatttg gtgatcgcca ctgtctttcg ctttgaagta
     2161 aagcggcgac cagtaagcgg taattgaaag tttatccgtt gcgtcattcc acaggtagta
     2221 accaccacct aagccacgga accagaagtt atcgccttca tagttgatta ccggtactgg
     2281 gtaaacatcg gtatcgtaat ctttatatgg gtgctcaacg acacctacgc ctgcgcccaa
     2341 ggaaaattta ccttcagcgt gcgctacgcc tgcagacgtt gcgataagca ctccaagtgc
     2401 cagaagtttg agtttggtca caattaatca ttccttaaac aaatgtttag cgggcgacaa
     2461 agtttacccg tcaatacatc gaaacccaac ctttttacgt tttcattttt taaagtaact
     2521 gtttaatttt cctgccgcgg atgacaccgc gcttacagcc aaatgaattt agcgttactg
     2581 gcgagcctgg tctttacatt aattatgcaa aatttatgga tgagttgttg atatgccatt
     2641 gaaattaaga aagccgtgca ggcaagtttt ccatttgcca tctacgctta attttgaagg
     2701 tgtatcaccg ggcacgttgt tctcatcgtc gataaaatgg catgagagtt gctgtgtttt
     2761 agcaagagac gtcgttcagt ttacctcttc cgggaggctc tactattcat atgaacggct
     2821 cttaacctgt gctaaaaaac gaaaggacgg cataccatga atatattcga tcactatcgc
     2881 cagcgatatg aagctgccaa ggacgaagag ttcacactgc aggagtttct taccacttgt
     2941 cggcaagatc gcagtgctta tgccaacgcg gctgagcggc tattgatggc tatcggtgag
     3001 cctgtcatgg tcgatacagc ccaggaaccc agactttctc gactcttttc taaccgggtc
     3061 attgcacgtt atccggcgtt tgaagagttt tacggcatgg aagacgcgat tgaacagatt
     3121 gtctcttatc tgaaacacgc ggctcagggg ctggaagaga agaaacaaat cctgtatctg
     3181 ctggggcctg tgggtggggg taaatcatcg cttgctgagc gactgaaatc attaatgcag
     3241 ctcgtaccga tttatgtatt gagcgccaac ggtgagcgta gcccggtcaa cgatcatccg
     3301 ttctgtcttt tcaatccgca ggaagatgcg cagattctgg aaaaagagta tggcattcct
     3361 cgccgttatc tcggcaccat catgtcgccg tgggcggcaa aacgcctgca tgaatttggt
     3421 ggcgatatca ctaagttccg ggtagtgaag gtctggccgt caattctgca acaaattgct
     3481 atcgccaaaa cggaacccgg cgatgagaac aaccaggaca tctccgcgct ggttgggaaa
     3541 gtcgatattc gtaaactcga acactacgcg cagaatgacc cggacgccta cggctattcc
     3601 ggtgcgctgt gccgcgccaa tcaggggatc atggaattcg ttgagatgtt taaagcaccg
     3661 ataaaagtgc tgcatccctt gttaaccgcc actcaggaag gtaactacaa cgggacggaa
     3721 ggtatctccg ccctgccgtt caacgggatt attctcgccc actcgaacga gtccgaatgg
     3781 gtcactttcc gtaataacaa aaacaacgaa gccttcctcg accgtgttta catcgtgaag
     3841 gtgccgtatt gcttgcgcat ttccgaagag atcaaaatct acgagaaatt gcttaatcac
     3901 agtgaattga ctcacgcccc atgcgcccct ggcacgctgg aaacactgtc acgtttttcc
     3961 attctttcgc gcctgaaaga gccagaaaac tccagcattt attcaaagat gcgggtttat
     4021 gatggcgaaa gtctgaaaga caccgatccc aaagccaagt cgtatcagga atatcgtgac
     4081 tacgccggtg tcgatgaagg gatgaacggt ctgtcgacgc gttttgcgtt taagatcctc
     4141 tcccgcgtgt tcaacttcga tcatgtagaa gtggcggcaa acccggtcca tctgttctac
     4201 gtcctggaac agcagatcga gcgcgagcag ttcccgcaag agcaggcaga acgctatctg
     4261 gagttcctga aaggttatct gatcccgaaa tatgccgagt ttatcggcaa agagatccag
     4321 acggcctacc ttgaatccta ttccgaatat gggcaaaaca ttttcgaccg ttatgttacc
     4381 tacgcggatt tctggattca ggatcaggag tatcgcgatc cggataccgg gcagctgttt
     4441 gaccgcgagt ctcttaacgc cgagctggag aaaatcgaga aaccggcggg gatcagtaat
     4501 ccaaaagatt tccgcaacga gattgttaac ttcgtactgc gcgccagagc gaataacagc
     4561 ggacgcaatc cgaactggac cagctatgaa aaactgcgca cggttatcga gaagaaaatg
     4621 ttctccaata ccgaggagct gttgccggtt atctcgttta acgccaaaac gtcaaccgac
     4681 gagcagaaga aacacgacga ctttgtcgac cgtatgatgg aaaaaggcta cacccgtaaa
     4741 caggtgcgtt tactgtgcga atggtatttg cgcgtacgta aatcgtctta acaaccctgg
     4801 cccggtacac atgctaccgg gcctacaacg acagcgaacc gtgggccaga gaaatggcaa
     4861 cacaggcgta gcatacagtt ggcaaatgta gtacgggggg catatgacct ggtttattga
     4921 ccggcgtctg aacggcaaaa acaaaagcat ggtgaatcgc cagcgttttt tacgccgtta
     4981 taaagcgcaa attaaacagt cgatctccga ggccatgaat aagcgttcgg tgactgacgt
     5041 cgacagcggc gaatccatat ccattcccac ggaagatatt agcgaaccga tgtttcatca
     5101 ggggcgtggc ggcctgcgcc accgcgtaca tccgggcaat gaccatttcg tccagaacga
     5161 ccgaattgaa cgtccccagg gtggcggcgg aggttccggc agtggtcagg gccaggccag
     5221 ccaggatggt gaaggtcagg atgaatttgt ctttcagatt tcgaaagatg agtatcttga
     5281 tctgctcttt gaagatttgg ccttaccgaa tctgaaacaa aaccaacaac gccagctgac
     5341 cgaatataaa acgcatcggg cgggttatac cgctaacggc gttccagcca atatcagcgt
     5401 tgtgcgttcc ttgcagaact cactggcgcg acgcacagcc atgacggcgg gcaagcgacg
     5461 ggaacttcat gcgctggaag agaatttggc catcatcagc aacagtgaac ctgcgcaact
     5521 gctggaagag gaacgtctgc gcaaagaaat tgcagaatta cgtgccaaaa ttgaacgcgt
     5581 cccttttatt gacaccttcg atttacgtta caagaactac gagaagcggc ccgatccctc
     5641 cagccaagca gtgatgtttt gcctgatgga cgtttccggt tcaatggatc aatccactaa
     5701 agatatggct aagcgttttt atattctgct gtatctgttc ctcagcagaa cgtataagaa
     5761 cgtggaagtc gtatacatcc gccatcatac ccaagcgaaa gaagtcgatg aacatgagtt
     5821 tttctactcg caggaaacag gcggcaccat tgtttccagc gccctgaaac tgatggatga
     5881 ggtagtgaaa gagcgttata acccggcaca gtggaatatt tacgctgcac aagcatcgga
     5941 cggcgataac tgggccgatg actctccgct ttgccatgaa atcctggcga aaaaattatt
     6001 acctgttgtt cgttattaca gctatatcga aattacccgt cgtgcacatc agacattgtg
     6061 gcgagaatat gagcatctgc aatctacttt cgacaacttt gcgatgcagc acatccgcga
     6121 ccaggatgat atttatccgg tgttccgtga actgtttcat aaacaaaatg caacagctaa
     6181 agactaaaac tatcagccag gtcattatcg cctggctgat ttttagctta ctgtaaatta
     6241 tctcatttat tacatacatt agcttacaat cgctttaaat atgacagcat aacctttaca
     6301 taatttagtt ccagaaaaca atcattcgga aaaatgattc agtcaacacg tatttccatg
     6361 gggttattct ttaaatattt tttatcgtta acgaaaattg atcctagtca aaactatata
     6421 tctctgccat caataaaatc cagcactcac attgctctcc tttttatggt ttctatgggt
     6481 acacaaaaat taaaagctca aagctttttt attttcagtt tattgctgac attaatttta
     6541 ttttgcatta ctaccttata taacgaaaac acaaatgtaa aactcatccc acagatgaat
     6601 tacctgatgg ttgttgtggc tttgtttttc cttaacgccg tcatttttct tttcatgtta
     6661 atgaaatatt tcactaacaa acaaatttta ccaacactca ttttaagcct tgcattctta
     6721 agtggcctta tctatttagt tgaaaccatt gtaattatcc ataaaccaat taacggcagt
     6781 acactgatcc agacaaagtc gaatgatgtt tctattttct atattttccg ccaactcagt
     6841 tttatttgtt taacctcgct ggcactcttt tgttatggaa aagacaacat ccttgacaac
     6901 aataagaaaa aaacgggaat cctgttgctg gcgttgattc cttttttagt ttttcccctt
     6961 ctggcacaca atctgagcag ttataacgct gactattctt tgtatgtcgt cgactactgt
     7021 ccggacaacc atactgcgac caggggaatc aactatacaa aaatattggt ttgtctgtgg
     7081 gcatttttac tgttctttat tatcatgcgt acacgattag ccagcgaact atggccgtta
     7141 atagcattat tatgtctggc atcgctatgc tgcaacttac ttctactgac tctggatgag
     7201 tataattaca ccatctggta tatcagtcgc gggattgaag tttccagtaa actgtttgtt
     7261 gtgtcttttc tgatttataa catttttcag gagctgcaac tctccagcaa actggcagtt
     7321 catgatgtgc tgaccaatat ttataatcgg cgctactttt tcaacagcgt agagtcatta
     7381 ttgtcgcgac ctgttgttaa ggacttctgt gtcatgctgg ttgatattaa tcagttcaaa
     7441 cgcatcaatg cccaatgggg acatcgtgtg ggtgataaag tgctggtttc aattgtcgat
     7501 attatccagc aaagcatccg ccctgatgat attttagcgc gactggaggg tgaggtgttt
     7561 ggcttgctat ttaccgaact caatagtgcc caggcaaaaa tcattgcgga tcgtatacgt
     7621 aaaaatgtcg aactcctgac cggctttagt aacagatatg atgttcctga acaaatgacc
     7681 atcagtattg gcacggtttt ttcaacgggt gacacgcgta atatctcgct tgtcatgacg
     7741 gaagcagata aagccttacg cgaagcgaaa agcgaggggg gcaacaaagt gattattcat
     7801 catatttaag tgcaaaaata ttcagagcca tgctttttgc gtggcttttg catacaattt
     7861 attactatta cccctaaatt tcccctcccc agcggcgcag agatgagtat aattagcgcc
     7921 cctgtgccag gccgcaatcg aactttatct ggttttctcg tttcactaac tgaaggagtg
     7981 ccatttatca tgaaattgca ccatagaatg ctccggcatt ttatcgccgc aagtgtcatt
     8041 gtgctgacat cttccttcct tatttttgaa cttgtcgcca gcgacagagc aatgagtgcc
     8101 tatctgcgct atatcgtgca gaaagcagac tcctcctttc tttatgataa gtatcagaat
     8161 cagagtattg ccgcgcatgt gatgcgcgct ctcgctgctg agcagtcgga agtgtcgcca
     8221 gaacagcggc gcgccatctg cgaggctttt gagtctgcca ataataccca tggcttaaac
     8281 ctgactgctc ataaataccc gggcttacgc ggcacactac aaaccgcatc cactgactgc
     8341 gacacaattg tggaagctgc agcactatta cccgcttttg atcaggcagt ggaaggcaac
     8401 cgccaccagg atgattacgg ttcaggtctt gggatggccg aagagaaatt tcactattat
     8461 ctcgatctca atgaccgcta tgtctatttt tatgagccgg ttaatgttga atactttgcg
     8521 atgaataact ggtccttcct gcagtcagga agtattggta tcgatcgcaa agatattgaa
     8581 aaggtattta ccgggcgtac cgtattgtcg agcatttacc aggatcagcg tactaaacag
     8641 aacgtgatga gtttgctgac gccggtgtat gttgcagggc agctaaaagg gattgtgctg
     8701 ctggatatta acaaaaacaa tttgcggaat atcttttaca ctcatgaccg ccctctcctc
     8761 tggcgttttc tcaatgtcac gctaaccgat accgattcgg ggcgtgaagt ggccaaattc
     8821 agtgtgccac ttc
//
LOCUS       DAJTNN010000166         8681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTNN010000166 DAJTNN010000000
VERSION     DAJTNN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.166"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            187..1020
                     /gene="ppsR"
                     /locus_tag="OO150_003411"
     CDS             187..1020
                     /gene="ppsR"
                     /locus_tag="OO150_003411"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5517438.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRTANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            1176..2222
                     /gene="aroH"
                     /locus_tag="OO150_003412"
     CDS             1176..2222
                     /gene="aroH"
                     /locus_tag="OO150_003412"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5517439.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARTSHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2354..2545
                     /gene="hemP"
                     /locus_tag="OO150_003413"
     CDS             2354..2545
                     /gene="hemP"
                     /locus_tag="OO150_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5517440.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2549..3985)
                     /locus_tag="OO150_003414"
     CDS             complement(2549..3985)
                     /locus_tag="OO150_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5517441.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNIELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLIQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(4048..4761)
                     /gene="ydiV"
                     /locus_tag="OO150_003415"
                     /pseudo
     CDS             complement(4048..4761)
                     /gene="ydiV"
                     /locus_tag="OO150_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            complement(5007..5471)
                     /locus_tag="OO150_003416"
     CDS             complement(5007..5471)
                     /locus_tag="OO150_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5517442.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5549..6298)
                     /gene="btuD"
                     /locus_tag="OO150_003417"
     CDS             complement(5549..6298)
                     /gene="btuD"
                     /locus_tag="OO150_003417"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5517443.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            complement(6298..6849)
                     /gene="btuE"
                     /locus_tag="OO150_003418"
     CDS             complement(6298..6849)
                     /gene="btuE"
                     /locus_tag="OO150_003418"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5517444.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(6912..7892)
                     /gene="btuC"
                     /locus_tag="OO150_003419"
     CDS             complement(6912..7892)
                     /gene="btuC"
                     /locus_tag="OO150_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5517445.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISLGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELLIGVVTATLGAPVFIWLLLKARR"
     gene            complement(8101..8430)
                     /locus_tag="OO150_003420"
     CDS             complement(8101..8430)
                     /locus_tag="OO150_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517446.1"
                     /translation="MNDDLERRVSCLESDVTEIKNNLITLTTRSESFATKSDVLEIRE
                     GLRLEMAESRQSLKSEMADLRQSLKVEMAEHRTELQKSFANQTWLLTGIVLSAMAVLV
                     AVVTVIK"
BASE COUNT         2286 a         2300 c         1984 g         2111 t
ORIGIN      
        1 aacctgctct attcgacaat ttccacataa accatcggta taaacgaaac cataaaaatg
       61 aaatgctgtt ttcataaaaa ataaaattga aggttcattt tataaaccag acattacctc
      121 ctacgcttct gtgcgttttt aatttatgct ttcatagaat tatgtctgca tcacgggaag
      181 aacaaaatgg ataatgctgt tgatcgccac gttttttata tttctgatgg tacggcaata
      241 actgcggagg tattaggaca cgcagtaatg tcacaatttc ccgtcactat cagcagcatt
      301 acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca gattgacgca
      361 atttatcacc agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt gccggagatt
      421 cgcgccatca tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc gctggttgcc
      481 ccgctacaac aagagatgaa actggatcca acgccgattg ctcatcgtac ccatggcctt
      541 aaccctaata atctcaataa atatgatgcg cgcattgcgg cgattgatta cacccttgcc
      601 cacgatgacg gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt
      661 tctcgctgtg gtaaaacccc caccagtctg tatctggcaa tgcaatttgg tatccgcacg
      721 gcaaactacc cctttattgc cgacgatatg gataatctgg tgctacccgc gtcgctcaaa
      781 ccgcttcagc ataaattgtt cggcctgact atcgacccgg aacgtctggc ggcgattcgc
      841 gaggaacgtc gggagaacag tcgctatgcc tcgcttcgtc agtgcagaat ggaagtcgcg
      901 gaagtggaag ccttgtaccg taaaaatcag atcccgtgga ttaacagtac caattattcg
      961 gtagaagaga ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg aatgtactag
     1021 agaactagtg cgctggtttg ttttttgtta tcatgctaac cacccggcga ggtgtgacac
     1081 acctcgcact tgaaatcagc tgcgattggt ttatcgtgat gcgcatcact tcccggtagt
     1141 cctgccgtag aagcaacaaa tttctgagac ttgtaatgaa cagaactgac gaactccgta
     1201 ctgcgcgtat tgagagcctg gtaacgcccg ccgaactcgc gctacggtat cccgtaacgc
     1261 ctggcgtcgc cacccatgtc accgactccc gccgcagaat tgaaaaaata ctgaatggtg
     1321 aagataagcg actgttggtc attattggcc cctgctcgat ccacgatctc accgctgcaa
     1381 tggagtacgc cacccgtctg cagtcgctgc gcaaccagta ccagtcacgg ctggaaatcg
     1441 taatgcgcac ctattttgaa aaaccacgaa ctgttgtcgg ctggaaaggg ctaatctccg
     1501 atccggattt aaacggcagc tatcgggtaa atcacggtct ggagctggcg cgcaaattac
     1561 ttttacaggt aaatgagctg ggtgtcccta ccgcgactga gtttctcgat atggtgaccg
     1621 gtcagtttat tgctgattta atcagttggg gcgcgattgg cgctcgtact accgaaagtc
     1681 agatccaccg cgaaatggct tcagcactct cctgtccggt aggttttaaa aatggtaccg
     1741 atggcaacac gcggattgcc gtggatgcta tccgcgcagc ccgcaccagc catatgttcc
     1801 tctcgccaga caaaaatggt cagatgacca tctatcagac cagcggcaac ccgtatggcc
     1861 acattattat gcgtggcggc aaaaaaccga attatcatgc cgatgatatc gccgcagcct
     1921 gcgatacgct gcacgagttt gatttacctg aacatctggt ggtggatttc agccacggta
     1981 actgccagaa gcagcaccgt cgccagttag aagtttgtga ggatatttgt cagcaaatcc
     2041 gcaatggctc tacggcgatt gctggaatta tggcggaaag tttcctgcgc gaaggaacgc
     2101 aaaaaatcgt cggcggtcag ccgctcactt acggtcaatc cattaccgac ccgtgtctgg
     2161 gctgggaaga taccgaacgc ctggtcgaaa aactcgcctc tgcggtagat acccgcttct
     2221 gaatgcgtgc ccatgcctga tgggatgggc atttctgcgc aacttgttgt cttctcaaca
     2281 cattactgct tgctctggcc tgccataata ttgataataa gaatcattgt tatatcaatt
     2341 attattaatt tttatgcgtt atacggatag cagaaaactc acgcctgaaa cggatgccaa
     2401 tcacaagacc gcttccccgc agcccatccg gcgaattacc agccagacac tgttaggtcc
     2461 ggatggcaaa ctgattatcg atcatgacgg gcaagaatat ctgctccgta aaactcaggc
     2521 tggcaagctg ctgttaacta agtagccttt aactcgagca gctgacttcc agccgtttac
     2581 cccagtcagg tggacgactg acatagtcat catctctgtc gctgaaagga tttcgcaacg
     2641 cctcatgcag gcggtgcaat tccatcatat cacccttttc tgccgcttca atcgcccgtt
     2701 gcgccaacca gttgcgcaat accagagctg ggttaacgct ttgtatcagt tgctgacgct
     2761 cactatcgct aacctcgtct tgttgcaaac gcccccgata acgggcaaac cagtcatcaa
     2821 atgccgcacg atcaataaac tcatcacgta gcggtgacgc cgcgctgtgc tgctcggtca
     2881 gactcagcat gcggaatgtg cgggtataat cgctgcgctc tcgcgccatc agactgaata
     2941 attcattcag tagcgcgtta tcctcttttt gctccgtcat gaagcccagt ttctgccgca
     3001 tccgttgtcc ataatgcgtc aacaaaacct gctgatagct gtccagggcc tcattcaggg
     3061 catctacggc aacaaatggt gacaatgtct gcgccagacg ctgtaaattc cacaacgcga
     3121 cagcaggttg attatcaaag ctataacgcc cttgatgatc cgagtgatta caaataaaac
     3181 cgggttcgta atcatcaaga aaaccaaacg gcccgtaatc aagcgtcagt cccagcagcg
     3241 acatgttgtc ggtattcatc accccatgtg caaagccgac cgtctgccat tgggcaatta
     3301 acgacgcggt acgtgcgaca acatcgctaa accagagacg gtatttgtcc tcatcatctt
     3361 caagatgtga ccagtaatga cgaatagcaa agtcagctaa ctgacgaacc ttttccgact
     3421 cgcggcggta gtaaaaatgt tcgaaatgac caaagcgcag atgacttggt gccacacgca
     3481 tcagcatcgc gcctggctcc gccgtttccc gatacactgg cgaatcgctg gtgacgatac
     3541 ttaacgcgcg ggtcgtcgga atgcccagat aatgcatcgc ctcactggcg agactttctc
     3601 gtatcgtcga acgtaaaacc gcccgtccat cacccattcg cgaataaggc gtcaggccag
     3661 cacctttcag atgccagtcc attgtagtgc catcagcaag cagttgttcg ccgagtaaaa
     3721 tgccgcgccc atcacccagt tggcccgccc agacgccgaa ctgatgacca ctgtaaacct
     3781 gcgccagtgg tgacatacca ggcagtaggg cttcgccgcc ccagacacct gcgccatttt
     3841 taaacagcga cgatggaata ctcagcgtgt tagccagttc gatattatgc caaatcagcc
     3901 gggcattatt taaaggcgta ggggaaagtg ctgtataggt ttctggcaat tcatcgcgcc
     3961 agcgggtaac aaaagacagg gtcatagatc ctcctgtttg atagtgtaga cggttactct
     4021 cgtcttaaac accagcaaac agaagggtta tcgctgaacc aacgtcgtta tctgatttat
     4081 gggaacggca ggccacaggc atccctggag agcatggaaa tcgaaggggg taatctgtgc
     4141 caatatttct gccgtatcaa tgcctcccgc aatgatgcaa ttgcaacaag gggaaatttg
     4201 cgcctagatc gcacgaataa acggttcgaa ggagcggtgc gtgatttgtt gctgaataaa
     4261 acttttatcc agcatgacgc gggtgaaaag accatcaaaa accgctttca ttgtgctatt
     4321 gcctgctccc aaattaccta acacaagggg atacacttgc gataaggata aaagaccgcg
     4381 attatctttt ccttcattga gatgcgggta attctcatta atcaacagtt caataaacgg
     4441 atattttaat agttcgcctg cgtaattatc cctctctaat aataatgtag ccacttgtgg
     4501 cgttaaatta agccaggcaa agagtttatg ttgaataaaa aaatgttggc atgattttag
     4561 taattccaac tgctctgaaa ataattgcca atgttgctcc tcggtaagtt gcgctataac
     4621 ccgactcgtc gggatacgca ccgtgccatc ttcactggag aaatgggtaa ttaactcaac
     4681 gccgacaagg acttgctggt tatctcttat cggcaggaaa tagcaatcag aatgataaag
     4741 attctccaaa aaaatcttca tggtaaccgt ccctctcgaa ggatgttttt cagtatccgg
     4801 ctaaaaggga taaggccata agacataaca attacagaag gagtaacttt catttgttcc
     4861 atgttaacca ctttttcagg ggtctttttt tagattatcc tgattataaa cgaataatcc
     4921 atttacggaa tttttgtctg caaaatacta ctgtattcag gagtaaatgg acggcgatgt
     4981 atcatcgccg tccatttttc gacatactag attcgtctcg cctgccagaa atttttctgc
     5041 caatagacat tatcaagtga ggaacgcatc actcccttgc tggtagaggc gtggataaat
     5101 tggttgttgg tatcgtaaat acctacatgc aaaccatttt gtccggaccc cgttttgaaa
     5161 aagaccaggt cacctggcag caacttgtct ttatcaattt gcgtgccaat agaggcttgt
     5221 tgtttggttt cacggggcag ctgcaaatcg aaacgatcgc gcatcgtcac aaccacaaat
     5281 cccgaacagt ccacaccgcg ccgcgtcatg ccaccataac gatacggcgt gccatgccag
     5341 ctttgtagct ggtcgttcaa accggcaata acggtaatcg aatcagaaag tctggcattt
     5401 ggcggcggtg ctttatggtg gctacacccg gccagaaaca gtgctgtgat caaaataagg
     5461 cagaaacgca ttccgtacgg ttcctctgtt ttttattctt gcattaattt agcgtcgtaa
     5521 ttacccgatt ttcaagatac taatgaaatc agatggtcaa aatcagcatt ctgtgacctt
     5581 cgatatccag acggcgaaaa ttcatcccat aggcctgcgc cagatttggc ggcgtgagca
     5641 cctcttctct gcgtccactg gccagcattt ttccaccttt tagcaaccac gcccgatgcg
     5701 catgacgcaa cgtgtggttg agatcgtgac tgctcatcac aatcgccagt ccttgctgac
     5761 acagcgcgct cagaattttg tctaacgcac tttgttgcgc aacatcaaga ctgttcatcg
     5821 gctcatcaag aagcagcaat tggcctgcgg gattggcttg tggtgtgatt tgcaacacca
     5881 ccgcagcaag acgtacgcgt tgccattcac cgccggaaag ttgattggtg ctacgaccga
     5941 gtttgtcatc aagagccagc gcccctgcga catcattcag tagttcggta cgcgttttat
     6001 cgtgctgatg cagtgtcagg tagtgccaga ccggcattgc aaacggcggc gtctgctgtt
     6061 gtgaaagata ggcgcgatgc agcgcgagtt ttgttgcgga ccatgcttcc aggggttgcc
     6121 ccgcgaactg aatgcttccc ttaccgctgg tcattccggc cattcgtgca agcaaggtac
     6181 tcttacccgc gccattcggc cccaccaggt gcaggatctc cccagcccga acctcgccag
     6241 aaagtggccc caggcgggta gattccgcaa catcctgtaa ctgcatcaca atagacatta
     6301 ttttgccaac gccagtttaa tgctttccat cacaatgggg tcttccggcg tcatatccgg
     6361 ggaaaaacgc tggatgacca atccgtccct gccaactaaa aatttctcaa aattccataa
     6421 aatatcatca gggtacagcg gtgcacgacc tttgctgacc atacgggcat agaatccgct
     6481 ctcttccggc gcgactgcgg tcggcgctgc ggcaatcaat ttttgataca gcggatggcg
     6541 tccttcgccg ttaacttcaa tcttactgaa catcgggaac gtcacccccc atgtggtggt
     6601 acagtaagtt ttaatctctt catcgctgcc cggttcttgt tccagaaact ggttgcacgg
     6661 gaatcccagc accacaaaac ctcgatcggc ccaggctttc tgaatattct ccaactgctc
     6721 atattgcggc gttaagccac actttgaggc gacattgaca atcaacagca cattaccggc
     6781 gtacttctcc agcgtggtca cttcaccgtc gatatctttc actacggtcg tcagaatgga
     6841 atcttgcatc gtttctcctg ggtgtggtca gtaaaaatct tagcttttaa tcatagaccg
     6901 tctttttgcg gctaacgtct tgcttttaac aataaccaga taaacaccgg cgcacccaac
     6961 gttgcggtga ccacgccaat aagcagctct gcggcagcta atgccaagcg cgctacaata
     7021 tcggccagca gcaatgcgct cgcccctgcc agcgcgcagc cgggaagtaa tacgcgatga
     7081 tcggttaaac cacacaaccg aagaatatgt gggatcacca gaccaataaa gccgatagca
     7141 cccgccagcg ccacactgac gccaaccatc cagccggtcg cggccaccag cacattgcgc
     7201 cagaaccaca ggggtaaacc cagttgccgc gccgagatct cgccaagtgc taacatattc
     7261 atcggcctgg actgacaaca gatccacaac aacacgggga tcaatgccag catcagccag
     7321 ctttgccgcc agtctacgcc gccaaaaccg cccatcatcc agtacatcag ctgacgtaaa
     7381 tcaacagagg tggaaaagta gatagcccac gtcattagtg cgctacagat aatccctaat
     7441 gcaacgccag ccagcaataa ccgactggtc gaaagatgac gacgggcgaa acgtaagagt
     7501 attaaagtaa tgataagcgc gccagcaatc gcacacagcc ctagcgccca gttggggagt
     7561 tgcccttgcc caagcaatac cgcggcgata agccccacgc ctgcgccgtt agagacgcca
     7621 agtagtccag gttctgccag aggattttca aacaacgcct gcattacagc gccggatata
     7681 gccagcgccg caccaaccaa caatacagcc agcgtacgtg gcaggcgaat ttgccagacg
     7741 aacagttcgc cacgaggagt aaaccagtca cctagcgaga tccattgttc accggcgcaa
     7801 aggcttaaaa gaagcgccag cagcatcaaa actgacaggc ataataacca gcgaatattt
     7861 tgtcgctgtt gttggcgggc aagtgtcagc atggtatccg ttctgctgaa gtgtcatggc
     7921 gttgatttta cggtgactct tcgacagtga aaagaaaaaa ggccgcagag cggccataaa
     7981 cacaagtaaa aatcaacaag ttagataatt atcaaagact tacagacaca caaaaacaca
     8041 gccaaccaca acaaataaca gggatgtggt cactttgtgg atcatatcgc cgccataaat
     8101 ttacttaata acagtaacca cagccacaag cacggccatc gcagacagga caatacctgt
     8161 gagtaaccaa gtctgatttg caaatgattt ctgaagttca gtacgatgct cagccatctc
     8221 aactttcaga gactggcgta aatcagccat ctcagacttc agggattggc gcgattctgc
     8281 catctcaagc ctcaaccctt cgcgaatctc cagtacatca gatttggtag caaaggattc
     8341 gcttcttgtg gtgagagtaa tcaggttgtt tttgatttca gtcacatcac tttccaggca
     8401 actgacccgc cgttcaaggt cgtcattcat gccatcacct ccatcattac cacttcctgt
     8461 tacttggttg ttggatacat tataggcatt ctctttcttt tttctgaacc gcgaaatatg
     8521 tgcaacattc tctgccatca cttctcatca ccctgcttgc ctacccagtt cataaaggcc
     8581 cgggccgagt aacgatgaac aaacccacat ctgttacagg taagtcggaa ttcatagtta
     8641 tggatatttt cccgatcatg tccctcatat cccggatacc c
//
LOCUS       DAJTNN010000167         8663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTNN010000167 DAJTNN010000000
VERSION     DAJTNN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.167"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..181
                     /locus_tag="OO150_003421"
     CDS             <1..181
                     /locus_tag="OO150_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5517447.1"
                     /translation="MMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLAT
                     LPSGDKVAAAVGITP"
     gene            complement(286..822)
                     /gene="rcdA"
                     /locus_tag="OO150_003422"
     CDS             complement(286..822)
                     /gene="rcdA"
                     /locus_tag="OO150_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5517448.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            906..2114
                     /locus_tag="OO150_003423"
     CDS             906..2114
                     /locus_tag="OO150_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5517449.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            2114..2929
                     /gene="ybjI"
                     /locus_tag="OO150_003424"
     CDS             2114..2929
                     /gene="ybjI"
                     /locus_tag="OO150_003424"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5517450.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            3015..3299
                     /locus_tag="OO150_003425"
     CDS             3015..3299
                     /locus_tag="OO150_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517451.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3340..4570)
                     /gene="mdfA"
                     /locus_tag="OO150_003426"
                     /pseudo
     CDS             complement(3340..4570)
                     /gene="mdfA"
                     /locus_tag="OO150_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            4855..5451
                     /gene="ybjG"
                     /locus_tag="OO150_003427"
     CDS             4855..5451
                     /gene="ybjG"
                     /locus_tag="OO150_003427"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5517452.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5509..6267
                     /gene="deoR"
                     /locus_tag="OO150_003428"
     CDS             5509..6267
                     /gene="deoR"
                     /locus_tag="OO150_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5517453.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6314..7516)
                     /gene="dacC"
                     /locus_tag="OO150_003429"
     CDS             complement(6314..7516)
                     /gene="dacC"
                     /locus_tag="OO150_003429"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5517454.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKDVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            7763..8388
                     /gene="gstB"
                     /locus_tag="OO150_003430"
                     /pseudo
     CDS             7763..8388
                     /gene="gstB"
                     /locus_tag="OO150_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(8385..>8663)
                     /locus_tag="OO150_003431"
     CDS             complement(8385..>8663)
                     /locus_tag="OO150_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HCR5517455.1"
                     /translation="PVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSV
                     NGDKVTEDGRILTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
BASE COUNT         2100 a         2217 c         2134 g         2212 t
ORIGIN      
        1 catgatgtcg ggtggaaaca ttctggcctt acaaaaaatt ctcggacacc acgatataaa
       61 aatgactatg cgttacgcac atctggcacc ggatcatctg gaaacggcgc tccgtttcaa
      121 tcctctggca acgctgccaa gtggcgacaa agtggcggca gcggttggca ttaccccgta
      181 ataaccacca ctgaccacca acctaactta ttgtttttaa tctaacttat tgttttcatt
      241 aacccgttta cataaatggg ttttttgtta tctaaaattt atctactacc ctgcaaccct
      301 ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta cggtcggtga caaaatgcag
      361 cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa accattgttc
      421 gagcgtttgc tgactgcgct gcatccagtt ttgcattacc gtttttaata gcggttttcg
      481 gctagccagc gcgtagagct ggtacatcag ctccatatta tccggcgttg caacctgtga
      541 gctgtagatc atatcggtga tagcctggca tgcgccttga gcatcactaa catcgctaaa
      601 aaatgcctga tattgccggg acataatctc agtaaaactg ctgaacgcct ccagcaacaa
      661 ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac ggtaccccgg caagggtagc
      721 aattttgcgg tgcgtaacag catgtattcc gtaaagtttc accgcctcca gcgtggcctg
      781 gataattttt tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc ctctgtctgc
      841 aattgtgtac aaatgtacac aaccttgcta atgttgtctt ccctcttctt attttgacgt
      901 gatctatgac cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg gcgctgttta
      961 tgttcttctt tttgccaggc ctgttaatgg cgtcctgggc aacccgtacg cctgctatcc
     1021 gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt tctctttggt ctgtcgatcg
     1081 gttcgatgag cggtattctc tgctcggcgt ggttagtgaa acgctttggg acacgtaatg
     1141 tcatcctggt cacgatgtcc tgcgcattga tcgggatgat gatattaagt ctggcactct
     1201 ggctgacatc gcccctgctc tttgccgttg gtctcggcgt ctttggggca agttttggtt
     1261 ctgcggaagt ggcgataaac gttgaaggtg ccgccgttga gcgagaaatg aataaaacgg
     1321 ttttgccgat gatgcacggt ttttatagcc tgggcacgct ggcaggcgct ggtgtcggga
     1381 tggcactgac ggcctttggc gttccggcaa cggtgcacat tttattggcg gcgctggtag
     1441 gtatcgcgcc tatttatatc gccattcagg caatccctga cggtacgggc aaaaatgctg
     1501 ccgatggcac ccagcatggc gaaaaaggca ttccttttta tcgcgatatc cagttgctgt
     1561 tgattggtgt tgtagtgctg gcgatggcct ttgccgaagg ttctgccaac gactggttac
     1621 ccttattaat ggttgacggt cacggtttta gccctacttc cggctcgctg atttatgccg
     1681 gttttaccct ggggatgacc gttggacgct tcactggcgg ttggttcatc gaccgttaca
     1741 gtcgcgttgc cgtggttcgg gccagtgcac taatgggggc gttgggtatt gggctgatta
     1801 tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt actctgggga ctgggtgcct
     1861 cgctgggctt cccgctgacc atttctgccg ccagcgatac cggccccgat gcaccaaccc
     1921 gcgtcagtgt ggtagcaacg accggttatc tggctttcct cgtcgggccg ccgctgctgg
     1981 gctatctcgg cgaacattat ggattacgta gtgcaatgct ggttgtactg gcgctggtta
     2041 ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg cagacggcga
     2101 tggagaatag ttgatgagca ttaaattaat tgcggtagac atggatggta ctttcttaag
     2161 cgatcaaaaa acctataacc gtgagcggtt tatggctcag tatcagcaaa tgaaagcaca
     2221 aggcattcgc tttgtggtcg ccagcgggaa tcaatattat cagttgatct ctttcttccc
     2281 tgaaattgct aatgaaattg cctttgtggc tgaaaacggc ggctgggtag tgagtgaagg
     2341 caaagatgtt tttaatggcg agctgtcgaa ggatgccttt actactgtcg tggaacattt
     2401 gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa aatagcgcct atacgctcaa
     2461 aaaatatgac gatgccatga aaacggtggc ggaaatgtat tatcaccgtc tggaatacgt
     2521 tgataacttt gacaacttag aagatatttt ctttaagttt ggcctgaatc tttccgatga
     2581 actgattcca caagtacaaa aagcattaca tgaggccatc ggcgatatta tggtgccagt
     2641 ccacaccggc aacggcagca tcgatctgat tatccccggc gtacataaag ccaatggcct
     2701 tcgccaactg cagaaattat ggggaataga cgacagcgaa gtggtggtct ttggcgatgg
     2761 cggtaacgat attgagatgc tgcgtcaggc aggctttagt tttgcaatgg aaaatgccgg
     2821 cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg aaggcgtact
     2881 ggatgtgatc gataaagtgc ttaaacacga agcgccattt gaccaataaa aacggcagca
     2941 cgcccggcaa cacaatacct gaatgaaggc tgcataattc gcggaaatta acccgctgca
     3001 ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt gggcgcgctt ttaatgggct
     3061 ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt gccgagtgga tacaaagtgg
     3121 ttgtagttcc tgattcagtc agcgttccac aagcggtcag cgtcgcgact gtgccgcaaa
     3181 ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc gcatccttat gctcgccatc
     3241 ttgccagcgt aggtgaaggg atggttatcg aacaccagat tgacgaccat caccattaat
     3301 tcgagaatgc ctgatcgcac aaagcagtca ggcatttttt tacccttcgt gagaatttcc
     3361 catctgttta tcttttaaaa agataaccat cagcgacagc cacaaaattc cgttgacaag
     3421 gttgaagaga ttaaactgtc cgttgccccc gttcagccag gcatgtttgc tgatttcaat
     3481 accaacggta aagatcagca tttgcagcat tcccatcgcc gcagaaaccg tacctttact
     3541 catatcgctg gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac caataccgaa
     3601 agcataaata cttaacccgg cagtcatcca taaatacgcg tgcgatgaga taaccgttgc
     3661 cgcagcagcg accaacagac caatcataat cggccagccg cccataatta tcagcgaacg
     3721 tacggtgcgg cacgaggtca gacgcgctaa cagcaagtta cccgcaatta acgccccgaa
     3781 aataggcact tgcagcaagc catattcata gctgctcaac tgctcgccgg taatgatgat
     3841 aatcggcgac tgggcgatcc acgccagcaa tggcaggcta acgaatccca gcgccagcgc
     3901 ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga gttctttcag
     3961 tgacagtttc tcgcctatac gcgtggcggt ttcaggcata gctcgttgca gaccgaaaaa
     4021 ggagatcgct gccaatgcgg caaacaaaac aaacatcccc tcccagggca gcacatggat
     4081 ccacgccgcg cccaccagcg gaccaagtag cggagcaatc agcgccacgt tcgccatcag
     4141 cgcggtgatc ttgatacaaa ccgcctcttc gaaagattcc tgaattgcgg cgtatcccac
     4201 agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagagtga attgttcaat
     4261 gttttgcgcc agtaatattg ccagacaggt gatgataaac cacaccaccc ccgccagcat
     4321 caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta aaaacatccc
     4381 gcccgccaga tatgcggtca ttgaagtagg aacccaatca atgcccgcct gatattgttc
     4441 caccacggcc aacataccgg gttgaatcat atctgccgat ataggttgaa aattcgtaaa
     4501 gcaccagaca gagagggaaa agtaacgcct gacgtccaag cctggcaccg gaagctaatt
     4561 tattttgcat gcaatttctt cgccaataat aatcgcgcag agtttaataa aagcgcagct
     4621 aacgagaaag cgaattttgt agctgaaacc acggttaagc acattcttac attattgcga
     4681 gtatagctac gctttcttta agttttattt aacctatgcc cgttacaatc acccaccgta
     4741 aacaggccgc ttgagggaaa taagacgatg ccgctttacc cagtttaacc tgcactttat
     4801 tctcaacgac ttgcctgtat tggctccctt ttaatcactt tgcgtcggga agttatgctg
     4861 gaaaatttga atctctctct attctctctt attaacgcga cgccagactc ggctccgtgg
     4921 atgatctcgt tggcgatttt tattgctaaa gatttgatta ccgtggtgcc gctgctggcc
     4981 gcggtgcttt ggttgtgggg gcttacagca caacggcaac tggtgataaa aatcgctatc
     5041 gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac atctttttcc gcacgaccga
     5101 ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg cggcggatga ctcattccca
     5161 agcgaccacg gtacggtgat tttcaccttt gcactggcat ttttatgctg gcatcgcctg
     5221 tggtccggct cacttttaat ggtgctggcc gtcgtcattg cctggtcgcg cgtttatctt
     5281 ggcgtccact ggccgctgga tatgctcggt ggattgctgg caggtatgat tggctgcctt
     5341 agtgcccaga ttatctggca agcgatgggg cataaactct atcaacgtct gcaatcgtgg
     5401 tatcgcttct gttttgcatt accgatccgc aaaggctggg tgcgtgactg atctctgcta
     5461 aaaagtgtag tattgagcgg ctcgcttcaa taactattca gagggattat ggaaacacgt
     5521 cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac gcagcgataa gttacatctt
     5581 aaagacgccg ccgccctgct tggggtttcg gagatgacga ttcgtcgcga tctgaacaac
     5641 cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc tggaaccgca tagtgccagc
     5701 cattacctgt taagcgatca aaaatcccgc ctggtggaag aaaaacgccg ggcggcaaaa
     5761 ctggctgcga cactggtaga acccgatcag accctctttt ttgactgtgg caccaccacg
     5821 ccgtggatta ttgaagcgat tgataatgaa atccctttta ccgccgtttg ttattcgcta
     5881 aatacctttc tggcgctgaa agagaaaccc cattgccgcg catttctgtg cggtggtgaa
     5941 tttcacgcca gcaacgccat tttcaaaccc atcgattttc agcaaacgtt gaataatttt
     6001 tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg tcagtaaagg cgctacctgt
     6061 tttaatcttg aagagttgcc ggtaaaacac tgggccatgt cgatggcgca aaagcatgtg
     6121 ctggttgtcg accacagtaa atttggcaag gtccgtccgg cgcgcatggg tgacctgaaa
     6181 cgctttgata ttgtggtgag tgattgttgc ccggaagatg agtatgtgaa gtacgcgcag
     6241 acgcaccgta ttaagttgat gtattaatga cgtataaccg gatgacgttt cgcgccatcc
     6301 ggttatcaga agattaagag aaccagctgc cgaaccactg atggaatttc atcatcacga
     6361 aatcccacat ccgaccaaag aatccgccct cttccacatt ttccatcacg atcagcggac
     6421 gctgctcaat ggatttaccg ttaagctgga aatcaatggt cccgacaacc tgaccttttt
     6481 tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct cgccttcagg tttttcagct
     6541 gcccacgcgg tatggtcact gagcccgctt cgcctgcccc gagattcact tcgctcttat
     6601 caccaaacca gacgcgctga gtcacaaagg tggcatcagg tttaattggc gtcacggttt
     6661 caaagaagcg gaaaccccag gtcaataatt tctcagactc attaaaacgg atacggtcag
     6721 ttttcgcccc caacactacg gagattaaac gcatatcgcc ctgggtagcc gaagcaacca
     6781 gattatatcc cgcgcctgcc gtggttcctg tcttcatgcc atcaacattc agattgctgc
     6841 tccacagcag acggttacgg ttaggctgac gaattttgtt gaaggtgaac tctttctctt
     6901 tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc tttacccagc aatgccatat
     6961 cgcgcgcggt actgaactgc cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt
     7021 tggtcagacc cagttttttg gcataaccat tcatcagacc aataaatgac tcctggctcc
     7081 cggcaacgta atcggccagc gcaatacagg cgtcattacc ggactggata atcacatctt
     7141 tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag gaacattacc gatgaaccac
     7201 gcagtgccgg atttcccgtc gcccaggcat ctttaccgac cgtcaccata tcggtgagtt
     7261 taatcttatc ggccttaagc gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc
     7321 tcgcgggatc cagtttctca tccgcattgc cttctgccag cactttaccg ctggcgtaat
     7381 ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc aacggtttgt tcggccgcga
     7441 atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc tgcaagacca cgaaggagag
     7501 aggagtattg cgtcataaat gccacccgag tatccattca aaaaaaatac gttatatcgc
     7561 cgtatgataa gttcggtgat taataacgca cacacaaact taaagaaacc gtaagttggt
     7621 aaagttttta aagtttatgc aatatcctgc cgcttcgctg cttctggtgt catttttttg
     7681 ccagctgttg cttaaaagtt aactttactt caccatcgga gtcctgttca gcctgtggct
     7741 gcctgtcgga aaaggggtga gtatgattac gctgtggggt cggaataatt caaccaacgt
     7801 aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat tctcgcgggc
     7861 cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa cgggctggtg
     7921 ccgttgttgc gtgacgacga aagtgatctc attctttggg aatcaaacgc cattgtccgc
     7981 tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg
     8041 gaagcggaaa aatggatgga ctgggcaaac cagacgctca gtaatgccca tcgagggatc
     8101 ctgatgggat tagtcagaac accaccggaa gagcgcgatc aggccgccat tgatgccagt
     8161 tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg
     8221 ttctctggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat ctacaatttg
     8281 tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta tcagcaactc
     8341 actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt gcgtgggcta
     8401 actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca
     8461 gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc ttctgtcact
     8521 ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc aataaataat
     8581 ttttgctgcc actgggggaa tttatcgctg ttatagaagg ccatgccgct cacagcgggc
     8641 gaatctttcc agtaaaaaac agg
//
LOCUS       DAJTNN010000168         8652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTNN010000168 DAJTNN010000000
VERSION     DAJTNN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.168"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..271)
                     /locus_tag="OO150_003432"
     CDS             complement(<1..271)
                     /locus_tag="OO150_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5517456.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(365..927)
                     /locus_tag="OO150_003433"
                     /pseudo
     CDS             complement(365..927)
                     /locus_tag="OO150_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1131..1337)
                     /locus_tag="OO150_003434"
     CDS             complement(1131..1337)
                     /locus_tag="OO150_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5517457.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1444..2007)
                     /locus_tag="OO150_003435"
     CDS             complement(1444..2007)
                     /locus_tag="OO150_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5517458.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3382..5217)
                     /locus_tag="OO150_003436"
     CDS             complement(3382..5217)
                     /locus_tag="OO150_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517459.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5318..5605
                     /locus_tag="OO150_003437"
     CDS             5318..5605
                     /locus_tag="OO150_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5517460.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5577..7106
                     /locus_tag="OO150_003438"
     CDS             5577..7106
                     /locus_tag="OO150_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5517461.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7276..8493)
                     /locus_tag="OO150_003439"
     CDS             complement(7276..8493)
                     /locus_tag="OO150_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5517462.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
BASE COUNT         2682 a         1955 c         1617 g         2398 t
ORIGIN      
        1 tacaccagac atcgttatta agtagcagca ccataagata aggagtggca tcgttagcca
       61 cagcttccct actccagagg taatataaag gggtgggctc gactgattta tctttacgtc
      121 gcttacactg caaatgttca gaaattagtc tatgcagctc accagtaaaa tcagcaatca
      181 gagaaggaat tgccttattc ataccagggc aaggtattaa tttaaactgt aataatttaa
      241 ttttaggatg tgtagcagca gcccgataca gagttgcaag gacacacttt tgccagaggg
      301 cgttacagga aagcttaatg tttgattctg tatacataat aaatcacctt atagttacaa
      361 taggtcaaaa accgctgtag ccaaagttac gctggcctga tgctttagtg tcgagcttcg
      421 tcaaataatc cagacgtacc aataagcgct ggtactgctc tgggaaatta ggatcgttaa
      481 tatccaggat gtaacgtcca ttagcaggga aatgaactaa tccctggtga tcttccagtt
      541 ccaaccctaa ggcactgtac caggctccgg taattaaccc tctcagggtg tcagagcgct
      601 catagtttcc cagatggtaa taggcgtctt tgttaaaaaa aggcagacgt gataatgaca
      661 tttaaaggac tgcgtatatt ctttcgccca gataatacaa accgacgttg cgtaggtgcg
      721 tttgccttgt tgttttttac ggtgttgatc tgcattaatt ttcgcattca gcgagttacg
      781 gaaacgagat atctctccgg ggatcaggtt agggaaacag gtaatgatat ccccgttgtc
      841 aatcacagga gggtaatgga gatccccccg tattgctgtt attctggaag tgtttttaat
      901 attcgtattg atagttctca gagatatatc acactctaga tattttaatt aagtatcagt
      961 tcgatatcgg catattgtac actacctcag gtagatgatg agatcagata cagggatcaa
     1021 ctgacggtga gcgcagtccc cacattttat ccagggctta ctgcatattc ccaacagcca
     1081 ttcgttagcc aggtagacga ataccctgat ttgccgctgg ttttgctttc ctatcgaagg
     1141 gcccatacat catcacgccc acgaaacaac cgacgaaata acgcaacttt ctcatccgtg
     1201 gataatacga aaacgcactg cacagggctc tgcggtttac gtcgccattc aatcccatga
     1261 gcttcaagta atgagaccag ccgggtattt tctgcccgca gtactgctag ttcatcgctg
     1321 tcagatttat aaaccatatg cgttatcact tgtacctgtc tcccggcggc accacgactg
     1381 aaattcgtgt gaaggcaaat ccacgcgata acttacgata gttcggacat aagcccccat
     1441 aattcagtta tgtcgtggtc gaccccggtt acttttttcg ggaagcctgc tacggcccag
     1501 aataataccg tgttctttct gatcctgttt agccagatag ctaagggcgt aacgtaagtc
     1561 gtctaccgca gatttatcac tatagtgaat cacgtgatcg atacgcaccg ggtatttgtc
     1621 tttagcccgg cacagatgaa aataaccctc cccttccgtg atccgcctcc agatatctcc
     1681 cagttgcctg gatatctgat aagagttctt gtgacgttgt ccatccaggt agccgataaa
     1741 atgaatatga agccctttgt ctggtgtata ttccattacc cagtaatatc ccgccaacat
     1801 cgtctgggtt tcgctaagta aacggtatat ttccatacac atactgtgct tacaggaatg
     1861 cccgaaactg ggcgtgtctt tcctgtaagc aaaatcaatt ctgaaaggta acagtttaga
     1921 aaaacgttga aacataccat ccatgtgttc attcacgtct ttcagaatca tgaaatccat
     1981 tttgtaattg ggattggcat tatacatttt atagtcctta ctttataaaa ctcacaggaa
     2041 gagagaatta cgtaaatact gagagtagag aaacgccgta ccacggcatt aataaacgaa
     2101 gtaaaccagc agcacagaca acctgataaa actgttttgt attaccgcat tactgtgatg
     2161 aggtaatact tcagaataca gtgagactct cttaaactga gaaccagtac gaattacaat
     2221 ggaaaaacac acctcataga attgagtttc aggaagcacc actaaattct ttacaggaag
     2281 taatctgaca ttcacaaaac tcagtatcgg ctagctacag gcaacatcgc tattaatatt
     2341 gtcgaaccat taagtaaagc cgagagagac cacgggtatt taatcattag cataaaagaa
     2401 taatggtata cagaacatac ccacacacca taagaagatt gttccataca gaaaagatca
     2461 tatttaccta tacctataaa ctgtaacgga gtcataacac gctctaatag tgttccgata
     2521 cgtaaccagt gttaagtacg tacaggacaa cattacacac ataattcacc atacagcttc
     2581 ccccatatgc ttcactgcgt agtgtatgga gatatatgca ttacacacat acagaatact
     2641 tagcataaag aggagtgaca ttaccacgta aagagataag ttatacaaca aacatacaaa
     2701 caatacggca cagacacaca ataccataac tccaactaat aatactaatt aaccttaatt
     2761 atttatggta tatgaaataa atgtgattct cccggaacta actaacctaa ccagacagat
     2821 gatcactgta taaaaacttc agttaatact catttgtttt gtcgactcag tatataatga
     2881 gtgaccagta atcaggataa tcacacttac ataacctgta aaaaagaaca agaagaataa
     2941 gccactaaca taatgcaaaa tggcatctga atcgatcgca gtttggcatt cgaatcgatc
     3001 atgatttggc ccaatccgtg atcgaataga gcggaatacg caacataatt caccagacaa
     3061 gagcttacac cgaattacag attatgtatt aatgtcaaaa ctggcatatt ttacagatac
     3121 aatgaacatg caacccatca atctaatagg ggcggtatgt acatctgccc atgaatggga
     3181 tggcgctaag aatacaaaac aattagacat agatcacaaa aacacaccat tctaattgaa
     3241 aaacaaaaca aacaaataaa tcatactatt atagaatttt ctcttgccag acatagataa
     3301 tacccctgct aattttgcta atggttagca aaattagcag ggtatataaa gattattgac
     3361 ccaaaacaca ctcagatgaa ttcacgacag ctcaggaggg ttattcctga tgtcatcttc
     3421 acaaataatg gttaacgcat catatatcat tctgcgtctg ttcctgtttt cggccttatc
     3481 gccatacaac atcatcacat ttttaatttc atcgaccggt gatttcacat agtgaactaa
     3541 tacaccaagc ttctcaacac attgcagtaa agtatatttc ttactgcctg tgaaaagccc
     3601 tatcatagtt tctttacgtt cgcgactgga taatactttg ttttccataa tgacttctgc
     3661 cagcgtctta ccatcataaa catactcttt atctatcaga atatttatct tatccagcac
     3721 cttctctacg tcatcaagca aacctgtaat atatgggggc gcaatatatg gtttcgtact
     3781 gatcttttca agcatacaat tatacatctt aataacacgc atcagggtaa aattgttcgc
     3841 atcatactgt atttcaccct ccttatatac aggatcaata agtggtattt tttcaataaa
     3901 tgcatcctga taggctttta catggtcaag ccaataaaac aacgagccat actttatatt
     3961 ttttctattt actttaacct ccagtgctgt ccgaagcaac gataatgccc ccctgacata
     4021 tcctgaaggt aattcaccaa acaatccttt tctttccatg tcatcataat gctgcagcac
     4081 atcattaatt ttatttttcc ttatcagaca aatgtacacc gcaagtgttt cgtagcagct
     4141 ggtcacatga acagcctcat tctgcaactg cccaaaatag cttataatct ttttcagata
     4201 tgcgtcatca caaccattct tatcattaat attaaataca agatctttca gacgggaaat
     4261 atattcctga gatttatcat caagtgaaac actataacga gacaatgttc cttctgcact
     4321 gttttgaaac ttataaaaca tttccagtaa tttttttgca taaggcgttg tatcaaagct
     4381 ggtaaagtca taaagatatt gcgcagcgta accatacaaa ccatgaatcc agagtccttc
     4441 tctcaccaac actctgagat aatatgcaag gctttgaagt atcttcgtct ttcgttgtat
     4501 atcaagtaag acacttaatg aataaaatgc attatagaat ttatcaatat tcctgagcac
     4561 tttctccagt tcatccttat ccagcttctt ctcccggtaa tcctttttat attcattttt
     4621 gatagccatg atatattgtt gcatcaggct cttgcttttc ccgttatcct tccgtatagt
     4681 tcctgtatat ttccccagag catacagctt tccattttca tgaaaatctt ccttgttgaa
     4741 tcccagttca gccagatatt cccgaagata acgtttaaca gcagcttcat cgctttctgt
     4801 atcagcgtga tcaagacaag gacaatacga tttctgtaaa aaattataaa tatgataata
     4861 aaaagcagag tcattctctg gtctgtatgt attatacata tctacaacaa gaaaaataac
     4921 cccaaaagtg cgcagacaat agctgcgaag gaattcaggt tttagtgtgg aatgcactgg
     4981 ataagaattc atcatgtaat aaaaatgttc atatcgggca tatatgatcc gccgttgctc
     5041 ctgactgaac accccttcag catcaataat acgactgatc tcattataac gttgatccca
     5101 gaatttttga tgcatttcaa aattggtact cttccgcttg tgtgccggat tctcgccttc
     5161 cttaaacaac cccaaaacag tacaaatatc atacaacata tttgtaaatg tgcgcatatg
     5221 ctgtgctctc cggttggttt gcattttatc gcagaggaca ttctgagctt aacgtatcaa
     5281 actgacacaa cgacatgcaa tgcatatgga gaacaatatg tcaaatcaaa ccggacagct
     5341 ttacacgcct tcacgcatcc tgcgtatggc tgaacttacc cgactgctgg gtatatcaag
     5401 atctactatt tacgtgaaaa taaatccggc ctcgaaatac cacgacccta cctttcccaa
     5461 accaataagg cttggtagtg catccgtagg ctggcgggca tctgccatcg atgagtggct
     5521 gatgttacat acggcaccag cctggtctga acctgaaaac aaactaaagg aacttaatga
     5581 tgacgacaaa actatcactc cctgaacttg acacaactaa actgcctgac aggtgccagg
     5641 cattacttga tgaaatgcat gaagaaacag gaatacgccg tgaaatactt ctgtctgtta
     5701 tgctgacggt catggctgcc tcggtccagg atacgcatga agttgaactt tccggagggc
     5761 aacgtaccag ccttcagata tttatgtgtc tttcatcagc atcaggcagt ggaaaaacct
     5821 ctgcctgcgc aaaattaatc gctcctgttc atgaaacaga agaagaacta catcaggcat
     5881 atatcgacga taaaaaaaac tatgaccgaa tgatggaaat gtggacaaca gataaaaaaa
     5941 tcctggagcg gagatataaa aaggaaattg aaagatcccc ggaaaatgct gctgctgcac
     6001 gagcagcact tgaaaagtgt atagcaaata aacccgaacc tcccgtacaa caggttctca
     6061 tcgtgaatga cgcgacacca gaagggatag ctttgaaact cagccagtca ccctctcttc
     6121 tgttgctgtc tgatgaagga ggtactattc tggataagcg tttcgaacgc aaatcggcac
     6181 tgtacaacac attatggagt gggcaaccag tcaacgttga acgggcatcc agacccgggt
     6241 tccggctgaa tgatgtccgt ctcactatac ttatactgac tcaaccggta atatttaata
     6301 agttttttac tctcactggc gatcagatcc ggggcaatgg ctttctggcc cggttgctat
     6361 tttgcgaacc cggagcaatt aaaataatga cgacagaacc gcatactgcc actcctgttg
     6421 tgacgcaaca atgtgcaagc tgttttcgta agaaaagttt tggcagtcag atcagagact
     6481 ctctgagagc atccagagaa agacgtgcaa aaagtaaaga gcgcatctgc atgacattat
     6541 caaatgcagc atcccgcgca ctggagcgct ttcacaaaga aaatatggat actgtcagac
     6601 agaatcctca tatgggtaca tttgaagaca ttattgtcag aaaaagagag caggttgtca
     6661 ggatagcagc acttctggaa ctggagaaag atccagactc gacagtaata acgctggaaa
     6721 gtactaatag cgccatttat ctcattgaat tttatttcaa gcatcttata tataaacttg
     6781 aatcccttcg ggaaatatcc ccggctgaga aacttgataa atggcttcag aaaagaatta
     6841 tcacaacagc aggttacata ttccagaaaa gttatatttt acaatatgcc ccatatgctc
     6901 tcaggaaaaa atgtgttctg gatgaagcac ttgacatact ggcggaacaa aggaaaataa
     6961 ggattgatga taacctggtt gtttatatag gaaacacgat tacaccatcc gaactggcaa
     7021 aaaaactaaa tattcctgca ttcgatgccg gagttttcat ttgtgatcat caaaacatac
     7081 tgaagtatca ccgaaacaga ctatagatac tatcggacaa aaaataaatt atcacaagtg
     7141 tattattcgt tataataatg aataatcgtt atctgagtgc aatgccaggt tttcattacg
     7201 aactggcatt gcatttattt ttcaaaaaag aaagcagtaa ccatttggct actctgagta
     7261 tgccagtcca tgaaactaag cgaattcctt agtgttttta gcaagttgag acatcacata
     7321 atccgcccat gcctgcatca tcggtacacg ctgctccagt agatcagtac gatgatatgc
     7381 cgcctcaacc ttatttttca gcgtatgagc gagcgccctt tccgccagat cccgcgaata
     7441 cccctgttca ctacaccagt ccctgaatgt tgagcgaaaa ccatgtgccg tggcaactcg
     7501 ccccggaatg tcactgacgg ctttcttttt acgcagaaaa cttgtcaaca ccatatcgga
     7561 aaggatctgc tgctttctgg gtgaagggaa caccagttca tcatgcaggc cacgtatatt
     7621 ttccagaatg taaatagcct gccgggataa aggaacacga tgctgtagcc tagctttcat
     7681 tctttctgca ggtatagtcc atacccgctt atgaaaatca atttcagccc agcgcattcc
     7741 cctggcttcg cccgagcgag ttgctgtaag tatcaccatt aataacagtg cgcgggtaac
     7801 attataaggt tcatcactat acacactggt cgccacaaaa agcggtaact gcctccaggg
     7861 cattgcgggt tggtgttcat cacgtcctct tgtctgctga ggaagcaaat ggtcaaccac
     7921 atcaacagga tttgccacac aaaaaccgtg cgcccatccc cactgcataa caacatgaat
     7981 gcgctgttta acccggcttg ccgtttctga caaggttaac cagactggac gcagtgtttc
     8041 tgccacgtcc gcagccgtaa tcgaatccag cgtttttgct cccagttgag gaaacgcgta
     8101 attctcaagc gtcgataacc actgccttac atgctttgga ttctcccatc caggagacag
     8161 ttctgcatgt acacgcctgg ctgcatcggc aaatgttggg ataacgactt tctcagattc
     8221 agccttttta atctccagag gatcatcacc tgcagcaagt tgctctcgca ttatccgggc
     8281 agtacgtgca gcttcagcaa tactgacctc tgggtaagtt cccaatccag cattacgtct
     8341 tttttgtgtc accggactta cataacgaaa aacccatttc ccccgcccct ttactgaaga
     8401 aggatgaagg gtcagtccgg taattccccc atggggcaat ggtttgtcat caggtttgat
     8461 atgtcttgct ttcgtatccg tcaatactgc catacgctaa ttcccgtcac tctggtatgc
     8521 catccagtat gccattagtc cctcgcttca ctaagaatac attagataac atcggacaac
     8581 aaaaagtgta atacattgtt tataaagaaa ttatataaca cccctggatt aagtcagatt
     8641 tatttcaggc gg
//
LOCUS       DAJTNN010000169         8647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTNN010000169 DAJTNN010000000
VERSION     DAJTNN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.169"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            157..1068
                     /gene="ttdA"
                     /locus_tag="OO150_003440"
     CDS             157..1068
                     /gene="ttdA"
                     /locus_tag="OO150_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR5517463.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1065..1670
                     /gene="ttdB"
                     /locus_tag="OO150_003441"
     CDS             1065..1670
                     /gene="ttdB"
                     /locus_tag="OO150_003441"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5517464.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRGKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1719..3182
                     /gene="ttdT"
                     /locus_tag="OO150_003442"
     CDS             1719..3182
                     /gene="ttdT"
                     /locus_tag="OO150_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5517465.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVFGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3225..4238)
                     /gene="tsaD"
                     /locus_tag="OO150_003443"
     CDS             complement(3225..4238)
                     /gene="tsaD"
                     /locus_tag="OO150_003443"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5517466.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4475..4690
                     /gene="rpsU"
                     /locus_tag="OO150_003444"
     CDS             4475..4690
                     /gene="rpsU"
                     /locus_tag="OO150_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5517467.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4801..6546
                     /gene="dnaG"
                     /locus_tag="OO150_003445"
     CDS             4801..6546
                     /gene="dnaG"
                     /locus_tag="OO150_003445"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5517468.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6741..8582
                     /gene="rpoD"
                     /locus_tag="OO150_003446"
     CDS             6741..8582
                     /gene="rpoD"
                     /locus_tag="OO150_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5517469.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2086 a         2311 c         2315 g         1935 t
ORIGIN      
        1 ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac gaactgttaa
       61 ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt ttatggcggt
      121 gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag taataagcaa
      181 caggcagtga ataagttgac agagattgtc gctaacttta ccgccatgat ttctacccga
      241 atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg
      301 gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga cctgaatcgt
      361 cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc ccgcttccca
      421 ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac cgtcaaagcg
      481 ccactacgtc acaatgcggt agaaattttt gacgaagtaa acaccggcaa aaataccggt
      541 agcggcgtac cgtgggtcac ctgggacatc atccccgaca atgacgatgc ggaaatcgaa
      601 gtttacatgg caggtggcgg ctgcacgcta cctggccgct cgaaagtgtt aatgccgtca
      661 gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac
      721 gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac cgccgccgta
      781 ctctcgcgta aagccatttt gcgcccgatt ggcagccgcc atcccaatcc aaaagcggca
      841 gaactggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc acaagggctg
      901 actggcaaca gttcagtgat gggcgtacat atcgaatctg ccgcccgcca tccgtcaacc
      961 atcggcgttg ctgtctctac cggctgctgg gcgcatcgtc gcggcacact gctggttcat
     1021 gccgatctca cctttgaaaa tctgtctcac acccggagcg cgttatgaaa aagatcctga
     1081 caaccccgat caaagctgaa gatctgcaag atattcgcgt cggcgatgtg atctacctga
     1141 ccggtacgct ggtgacctgc cgtgacgttt gtcaccgccg cttgatcgaa ctgaaacgtc
     1201 cgatccctta cgatctcaac ggcaaagcga ttttccacgc tggccccatc gtgcgcaaaa
     1261 acggcgataa atgggagatg gtttccgttg gcccgacaac cagtatgcgt atggaaagtt
     1321 ttgaacgtga atttatcgag cagaccggcg tgaaactggt ggttggcaaa ggcggaatgg
     1381 ggccgctgac cgaagaaggc tgccagaaat tcaaggcgct acatgtgatt ttcccggcag
     1441 gctgcgcggt gctggcggca acccaggtgg aagagattga agaagtgcac tggacagagc
     1501 tcggaatgcc ggagtcgctg tgggtctgcc ggggcaaaga gttcggcccg ctgattgtct
     1561 ctattgatac ccacggcaac aatctgatag ccgaaaacaa aaagctgttc gccgaacgcc
     1621 gcgatcccat cgtggaagag atctgcgagc acgtccatta catcaaataa ccctcccgga
     1681 gaggctcgcc ccctctcctt tttcgcaggc ataacacgat gaaaccttcc actgaatggt
     1741 ggcgatacct tgcgccgctg gcggtcatcg ccattattgc tctaattccg gttcccgcag
     1801 ggctggagag tcatacctgg ctctactttg ccgtttttac tggcgtgatc gttggactga
     1861 tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc atcgccatac
     1921 tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt aagttcaccg
     1981 ccaaatccct ctcctgggca gtttttggtt tttctaattc ggttatctgg ctcattttcg
     2041 ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc attgcgctga
     2101 ttctggtgaa aaagatggga catcgcacgc tgtttctcgg ctatgcggtg atgttctccg
     2161 agctgatcct cgcacctgta acaccgtcca actcggcgcg tggtgcgggg attatctatc
     2221 ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc agttcgcgca
     2281 gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc agcgccattt
     2341 tcttgacggc gatggcacca aacttgctgt taattggact gatgaaaagc gcatctcacg
     2401 ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagtatt ttgctggtgc
     2461 tgctggttcc ctggctggct tatgtgctgt acccgccggt actgaagtct ggcgatcagg
     2521 tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg cgtgaaaaac
     2581 ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt gattatatcg
     2641 atgccgcgat ggtcggttac agcgtggtgg cactgatgct gcttctgcgc attatcagtt
     2701 gggacgacat tgtcagtaat aaagcggcgt ggaacgtttt cttctggctg gcctcgctta
     2761 tcaccctcgc taccggactc aacaacactg gttttattag ctggtttggc aaactgttag
     2821 caggcagctt aagcggttat tcgccaacga tggtgatggt ggcgttgatt gtggtgtttt
     2881 atctgctgcg ctactttttc gccagcgcca cggcgtatac ctccgccctc gcaccgatga
     2941 tgattgccgc tgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc ctaatggttg
     3001 gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc agtccgattt
     3061 actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg atttttgggc
     3121 tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg gtgttgcttt
     3181 aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc agccggtaac
     3241 tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc gcctgcttta
     3301 aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa ctccggacgc
     3361 gcgtagaaca cttcgccgcg gcgttttttc atcatttccg ccagcttcgc ccgtaacgtg
     3421 cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg atccaacgct
     3481 cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg ggcgatatca
     3541 gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa ggttttcaga
     3601 ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa aacaaagcgc
     3661 ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata atccagcccc
     3721 agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga ctcgccgagc
     3781 agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc ggacaccagc
     3841 agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa cagatgccct
     3901 tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga acgccccacg
     3961 gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc cacagcatca
     4021 atgtcttttg ccgttaagcc agactccttt agcgccgcct ggatcaacgg tacggttttg
     4081 cgcacgtgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc gtgcaatttc
     4141 acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat ggcgatgccg
     4201 gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac ctcgctttat
     4261 taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc ctggagaaag
     4321 cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaggccgcg gtgctttaca
     4381 aagcagcagc aattgcagta aaattccgca ccattttgaa ataagctggc gttgatgcca
     4441 gcggcaaacc gaattaatca aaggtgagag gcacatgccg gtaattaaag tacgtgaaaa
     4501 cgagccgttc gacgtagctc tgcgtcgctt caagcgttcc tgcgaaaaag caggtgttct
     4561 ggcggaagtt cgtcgtcgtg agttctatga aaaaccgact accgaacgta agcgcgctaa
     4621 agcttctgca gtgaaacgtc acgcgaagaa actggctcgc gaaaacgcac gccgcactcg
     4681 tctgtactaa ttccccgaga gcgttgctct ccgatcagac cgagttgtag ttgtaaggcc
     4741 gtgcttccga aaggaatgcg cggcttattt tcgtttatga attgctaaaa atcggggcct
     4801 atggctggac gaatcccacg cgtattcatt aatgatctac tggcacgcac tgacatcgtc
     4861 gatctgatcg atgcccgtgt gaagctgaaa aagcagggca agaatttcca cgcgtgttgt
     4921 ccattccaca acgagaaaac cccgtcattc accgttaacg gtgagaaaca gttttaccac
     4981 tgctttggat gtggcgcgca cggcaacgcg atcgacttcc tgatgaacta cgacaagctt
     5041 gagttcgtcg aaacggtcga agagctggca gcaatgcaca atcttgaagt gccatttgaa
     5101 gcaggcagcg gccccagcca gatcgagcgc catcagaggc aaacgcttta tcagttgatg
     5161 gacggtctga atacgtttta ccaacaatct ttacaacaac ctgttgccac gtctgcgcgc
     5221 cagtatctgg aaaaacgcgg attaagccac gaggttatcg ctcgctttgc gattggtttt
     5281 gcgccccccg gctgggacaa cgtcctgaag cggtttggcg gcaatccaga aaatcgccag
     5341 tcattgattg atgcggggat gttggtcact aacgatcagg gacgcagtta cgatcgtttc
     5401 cgcgagcggg tgatgttccc cattcgcgat aaacgcggtc gggtgattgg ttttggcggg
     5461 cgcgtgctgg gcaacgatac ccccaaatac ctgaactcgc cggaaacaga cattttccat
     5521 aaaggccgcc agctttacgg tctttatgaa gcgcagcagg ataacgctga acccaatcgt
     5581 ctgcttgtgg tcgaaggcta tatggacgtg gtggcgctgg cgcaatacgg cattaattac
     5641 gccgttgcgt cgttaggtac gtcaaccacc gccgatcaca tacaactgtt gttccgcgcg
     5701 accaacaatg tcatttgctg ttatgacggc gaccgtgcag gccgtgatgc cgcatggcga
     5761 gcgctggaaa cggcgctgcc ttacatgaca gacggccgtc agctacgctt tatgtttttg
     5821 ccggatggcg aagaccctga cacgctggta cgaaaagaag gtaaagaagc gtttgaagcg
     5881 cggatggagc aggcgatgcc actctccgca tttctgttta acagcctgat gccgcaagtt
     5941 gatctgagta cccctgacgg gcgcgcacgt ttgagtacgc tggcactgcc attgatatcg
     6001 caagtgccgg gcgaaacgct gcgaatatat cttcgtcagg aattaggcaa caaattaggc
     6061 atacttgatg acagccagct tgaacgatta atgccaaaag cggcagagag cggcgtttct
     6121 cgccctgttc cgcagctaaa acgcacgacc atgcgtatac ttatagggtt gctggtgcaa
     6181 aatccagaat tagcgacgtt ggtcccgccg cttgagaatc tggatgaaaa taagctccct
     6241 ggacttggct tattcagaga actggtcaac acttgtctct cccagccagg tctgaccacc
     6301 gggcaacttt tagagcacta tcgtggtaca aataatgctg ccacccttga aaaactgtcg
     6361 atgtgggacg atatagcaga taagaatatt gctgagcaaa ccttcaccga ctcactcaac
     6421 catatgtttg attcgctgct tgaactgcgc caggaagagt taatcgctcg tgagcgcacg
     6481 catggtttaa gcaacgaaga acgcctggag ctctggacat taaaccagga gctggcgaaa
     6541 aagtgattta acggcttaag tgccgaagag cgatcgggaa gcccccgaca gccgcactga
     6601 gaggcagcgg caaatatata agtacgccct cgtaattatc gttggcggta aacaaccgtt
     6661 ggatttcagc gttaacggct gaaggacatc gggtcaatcg cccaacacca acctcatgaa
     6721 ataagtgtgg ataccgtctt atggagcaaa acccgcagtc acagctgaaa cttcttgtca
     6781 cccgtggtaa ggagcaaggc tatctgacct atgccgaggt caatgaccat ctgccggaag
     6841 atatcgtcga ttcagatcag atcgaagaca tcatccaaat gatcaacgac atgggcattc
     6901 aggtgatgga agaagcaccg gatgccgatg atctgatgct ggctgaaaac accgcggacg
     6961 aagatgctgc cgaagccgcc gcgcaggtgc tttccagcgt ggaatctgaa atcgggcgca
     7021 cgactgaccc ggtacgcatg tacatgcgtg aaatgggcac cgttgaactg ttgacccgcg
     7081 aaggcgaaat tgacatcgct aagcgtattg aagacgggat caaccaggtt caatgctccg
     7141 ttgctgaata tccggaagcg atcacctatc tgctggaaca gtacgatcgt gttgaagcag
     7201 aagaagcgcg tctgtccgat ctgatcaccg gctttgttga cccgaacgca gaagaagatc
     7261 tggcacctac cgccactcac gtcggttctg agctttccca ggaagatctg gacgatgacg
     7321 aagatgaaga cgaagaagat ggcgatgacg acagcgccga tgatgacaac agcatcgacc
     7381 cggaactggc tcgcgaaaaa tttgcggaac tacgcgctca gtacgttgta acgcgtgaca
     7441 ccatcaaagc gaaaggtcgc agtcacgctg ccgctcagga agagatcctg aaactgtctg
     7501 aagtattcaa acagttccgc ctggtgccga agcagtttga ctacctggtc aacagcatgc
     7561 gcgtcatgat ggaccgcgtt cgtacgcaag aacgtctgat catgaagctc tgcgttgagc
     7621 agtgcaaaat gccgaagaaa aacttcatta ccctgtttac cggcaacgaa accagcgata
     7681 cctggttcaa cgcggcaatt gcgatgaaca agccgtggtc ggaaaaactg cacgatgtct
     7741 ctgaagaagt gcatcgcgcc ctgcagaaac tgcagcagat tgaagaagaa accggcctga
     7801 ccatcgagca ggttaaagat atcaaccgtc gtatgtccat cggtgaagcg aaagcccgcc
     7861 gtgcgaagaa agagatggtt gaagcgaact tacgtctggt tatttctatc gccaagaaat
     7921 acaccaaccg tggcttgcag ttccttgacc tgattcagga aggcaacatc ggtctgatga
     7981 aagcggttga taaattcgaa taccgccgtg gttacaagtt ctccacctac gcaacctggt
     8041 ggatccgtca ggcgatcacc cgttctatcg cggatcaggc gcgcaccatc cgtattccgg
     8101 tgcatatgat tgagactatc aacaaactca accgtatttc tcgccagatg ctgcaagaga
     8161 tgggccgtga accgacgccg gaagaactgg ctgaacgtat gctgatgccg gaagacaaga
     8221 tccgcaaagt gctgaagatc gccaaagagc caatctccat ggaaacgccg atcggtgatg
     8281 atgaagattc gcatctgggg gatttcatcg aggataccac cctcgagctg ccgctggatt
     8341 ctgcgaccac cgaaagcctg cgtgcggcaa cgcacgacgt gctggctggc ctgaccgcgc
     8401 gtgaagcgaa agttctgcgt atgcgtttcg gtatcgatat gaacaccgac cacacgctgg
     8461 aagaagtggg taaacagttc gacgttaccc gcgaacgtat ccgtcagatc gaagcgaagg
     8521 cgctgcgcaa actgcgtcac ccgagccgtt ctgaagtgtt gcgtagcttc ctggacgatt
     8581 aatcggtagg ccggatcagg cgttacgccg cacccggcac tagtccctct gcacaaacgc
     8641 caccttt
//
LOCUS       DAJTNN010000170         8634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTNN010000170 DAJTNN010000000
VERSION     DAJTNN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.170"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..66
                     /locus_tag="OO150_003447"
     CDS             <1..66
                     /locus_tag="OO150_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5517470.1"
                     /translation="RYQAKYVSAIWQKLIPSQADF"
     gene            125..328
                     /locus_tag="OO150_003448"
     CDS             125..328
                     /locus_tag="OO150_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCR5517471.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENELPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            328..1353
                     /locus_tag="OO150_003449"
                     /pseudo
     CDS             328..1353
                     /locus_tag="OO150_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1381..1470)
                     /locus_tag="OO150_003450"
     tRNA            complement(1381..1470)
                     /locus_tag="OO150_003450"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1434..1436),aa:Ser,seq:cga)
     gene            1564..2361
                     /gene="mtfA"
                     /locus_tag="OO150_003451"
     CDS             1564..2361
                     /gene="mtfA"
                     /locus_tag="OO150_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5517472.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2462..2537
                     /locus_tag="OO150_003452"
     tRNA            2462..2537
                     /locus_tag="OO150_003452"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2495..2497,aa:Asn,seq:gtt)
     gene            2851..>8634
                     /locus_tag="OO150_003453"
     CDS             2851..>8634
                     /locus_tag="OO150_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5517473.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYILGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFNNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPSWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVMVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGVAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLKALNTNENGIAEVTLKGTVLGVYTAEAILLNGKSDTKIVNIVPDTSNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGMHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTKPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     VEATLPNGNGYTTTVNIAPDTSNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNGVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEKTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGETTSLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQLTATLENGDSMQQTVTYVPNVANAEIKLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTAS
                     MTGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKA"
BASE COUNT         2471 a         2069 c         2106 g         1988 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagctatc tggcaaaaac tgatcccgag tcaggctgat
       61 ttttaatgtt caaccctaat tgccgctaac cgtatatagt tagcggcggt tatgagatat
      121 agctatgagc agcttatttt taaccgaaga tgaattgcta atattaacgg gctgcaaata
      181 tgcaagccac cagcgaaaat ggttaatgga aaacgagctt ccgttctata ccaatcgtag
      241 tggcaaaccg attgtcagcc gggatctatt tacctgcaat aaaactttac caccacgcga
      301 ggtagagccg aattttggtg cgatctgatg ggaagacgaa ggaaaaatcc tgagcacgaa
      361 aaattacctc caaatgtata cccaaataaa tatagttatg tatggaaacc aacatccagc
      421 gaatctgtaa cactaaccgc catcaaggat ggtttagctg ctttatggaa aaagtatgag
      481 gaaactgtaa ataatcgcga tcgtgcaatg acattcggtc gcttgtggga aaaattcctc
      541 gccagcgcct attacagtga cctcagtcca agaacacaaa aagattatct gcaacatcaa
      601 aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca gaacacatcc
      661 gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat gaagtcccgc
      721 gtttacagtt gggggtatga gcgagggtac gtgaaggcta acccatgtgc aggtgtaagt
      781 aaattcaagg ccaaaaaccg cgaacgatat gtaaccgaca aagaatacca ggcagtatta
      841 agcgttgcac ctcttcctgt ttttatcgca atggaaattg cctatctgtg tgcagcgagg
      901 gtttccgatg tgttatcgct gaaatgggag cagattggaa acgacgggat ctttatccag
      961 caagggaaaa caggaaaaaa acagataaaa gcatggagtc cacgattaca ggcggcgatc
     1021 gaaaaagcaa aacagttacc aacatccgcc tatgtaatca gcaatcaata cggcaaccga
     1081 tatatgtaca aaggctttaa cgaaatgtgg gtagaagcaa gaaatcgcgc aggcaaaatt
     1141 tcaggtattt taaccgactt cacctttcat gatctgaagg cgaaaggaat ttcagactat
     1201 gaaggaagca gtcgggataa gcaacttttc tctggtcaca aaaccgaggg gcaagtgcta
     1261 atctatgaca ggaaggttaa agtttcaccg acacttgatg tcccgttacc tgaaaatatt
     1321 ccaagtaatt ccacgtgtga tttttgtcac tgacttaatg atgtataagt gattgaattt
     1381 tggcggagag agggggattt gaacccccgg tggagttgcc cccactccgg ttttcgagac
     1441 cggtccgttc agccgctccg gcatctctcc gttcagatgg ttgccatgat gccaggaaat
     1501 ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa gtgacgagtt tgcgagcaaa
     1561 acgatgatta agtggccctg gaaagtacaa gaatcagcac atcaaactgc ccttccctgg
     1621 caggaagcac tatcgatccc ccttttaacg tgtctgacgg aacaggaaca aagcaaatta
     1681 gtcactcttg ccgaacgttt tttacagcaa aagcggcttg ttcctttaca gggctttgaa
     1741 ctggattcat taagaagctg ccggatagca cttctatttt gcctacccgt tctggagtta
     1801 ggactggaat ggctggatgg ttttcatgaa gtcttaattt atcctgcgcc atttgtggtc
     1861 gatgatgaat gggaagacga tatcggtctg gtgcataacc aacgtattgt tcagtcaggt
     1921 cagagctggc agcaagggcc tatcgttttg aactggttgg atatacaaga ttcttttgat
     1981 gcttctggtt ttaacctgat tattcatgaa gttgctcata agctggacac ccgtaacggc
     2041 gatcgcgcca gcggagttcc ctttattccg ttgcgtgagg ttgctggctg ggaacacgat
     2101 cttcatgctg caatgaacaa cattcaggaa gaaatcgaat tggttggcga gaatgcggcg
     2161 agcattgatg cttatgcagc cagcgatcct gctgaatgtt ttgccgtact ttctgaatat
     2221 ttctttagcg ccccagaact ttttgctcct cgtttccctt cattgtggca acgtttctgc
     2281 caattttatc aacaagatcc tttgcagaga ctgcatcacg ctaatgatac agactcgttt
     2341 tcggcgacga atgttcatta attaacaact ttgcatatta attaatcaat tgaaatgact
     2401 tatgaaattt agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc gttcacacga
     2461 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     2521 tccagtcaga ggagccaaat tctaaaaatt cgctttttta gcgcaatgtc actgacctta
     2581 gttgaacatt gttttttaac ggatagcggg tttttaacat cttaagcgcc ctcgaccttt
     2641 atggttgagg gcgttttgct atgaacgcca tcaccatttt cccctcgatt ataaaacttg
     2701 agttattcag tagtctcccc tcttgcaact cacacccaaa actgcctaac gaaaagttat
     2761 taattttcaa tcatattgct atcagtattt acattttttc gctgtgctag aaagggcgca
     2821 tttatgttag ctcgttcagg gaaggtaagc atggctacga agaagagaag tggagaagaa
     2881 ataaatgacc gacaaatatt atgcgggatg ggaattaaac tacgccgctt aactgcgggt
     2941 atctgtctga taactcaact tgcgttccct atggctgcgg cagcacaagg tgtggtaaac
     3001 gccgcaaccc aacaaccagt tcctgcacaa attgccattg caaatgccaa tacggtgccc
     3061 tacatccttg gagcgctgga atcggcccaa agcgttgccg aacgtttcgg tatttcggtg
     3121 gctgagttac gcaaactcaa ccagtttcgt acgtttgctc gaggttttaa taatgtccgc
     3181 cagggtgatg aactggatgt cccggcacaa gttagtgaaa aaaaattaac cccgccgccg
     3241 ggtaatagca gtgacaacct cgagcaacag atagccagta cttcacagca aatcgggtct
     3301 ctgctcgccg aagatatgaa cagcgagcaa gcggcaaata tggcgcgtgg atgggcctct
     3361 tctcaggctt caggcgcaat gacagactgg ttaagccgct tcggtaccgc aagaatcacg
     3421 ctgggcgtgg atgaagattt tagcctgaag aactcccagt tcgattttct ccatccgtgg
     3481 tatgaaacgc ctgataatct ctttttcagt cagcatactc tccatcgtac tgacgagcgt
     3541 acgcagatta acaacggctt aggttggcgt catttcactc ccacatggat gtcaggcatc
     3601 aacttctttt tcgaccacga tcttagccgt taccactccc gcgccggcat tggcgcggag
     3661 tactggcgcg actatctaaa attaagcagt aacggctatt tgcgactgac caactggcgc
     3721 agcgcacctg aactggacaa cgattatgaa gcacgcccgg ccaatggctg ggatgtacgc
     3781 gcagaaagct ggctaccctc ctggccgcac cttggcggta aactggtcta tgaacagtat
     3841 tatggcgatg aagtggccct gttcgataaa gacgatcggc aaagtaatcc tcatgccata
     3901 accgctggac ttaactatac ccccttcccg ctgatgacct tcagcgcgga gcaacgccag
     3961 ggtaaacagg gcgaaaatga cacccgtttt gccgtcgatt ttacctggca acctggcagc
     4021 gcaatgcaga aacagcttga cccgaatgaa gtcgctgcac ggcgtagcct tgcaggcagc
     4081 cgttatgatc tggtggatcg caacaacaat atcgttctgg aatatcgcaa aaaagaactg
     4141 gttcgcctga ccctgacaga ccccgtgaca gggaagtcag gagaagtgaa atcactggtt
     4201 tcgtcgctac aaaccaaata tgccctgaaa ggctataacg tcgaagccac cgcactggaa
     4261 gctgccggtg gcaaagtggt cacaacgggt aaagatattc tggttaccct gccggcttac
     4321 cggttcacca gtacgccaga aaccgataac acctggccga ttgaagtcac cgccgaagat
     4381 gtcaaaggca atttgtcgaa tcgtgaacag agcatggtgg tcgttcaggc acctacgcta
     4441 agccagaaag attcctcggt atcgttaagt acccaaacat tgaacgcgga ttcccattca
     4501 accgccacac tgacttttat tgcgcatgat gcagcaggta atcctgttgt cgggctggtg
     4561 ctctcgacgc gtcacgaagg tgttcaggac atcacccttt ctgactggaa agataatggt
     4621 gacggaagct atacccagat cctgaccaca ggagcgatgt ctggcacgct gacgctgatg
     4681 ccacagctga atggtgtgga tgcggctaaa gcccccgccg tggtgaatat catttctgtt
     4741 tcgtcatccc gaactcactc gtcaattaag attgataagg accgttatct ctccggcaat
     4801 cctatcgagg tgacggtaga actgagagat gaaaatgaca aacctgttaa ggaacaaaaa
     4861 cagcaactga ataacgcagt cagcatcgac aacgtgaaac caggagtcac tacagactgg
     4921 aaagaaaccg cagatggcgt ctataaggcg acctataccg cctataccaa aggcagtgga
     4981 cttactgcga agctattaat gcaaaactgg aatgaagatt tgcataccgc tggatttatc
     5041 atcgacgcca acccgcagtc agcgaaaatt gcgacattat ctgccagcaa taatggtgtg
     5101 ctcgccaatg agaatgcagc aaacaccgtc tcggtcaatg tcgctgatga aggaagcaac
     5161 ccaatcaatg atcataccgt cacgtttgcg gtattaagcg gatcggcaac ttccttcaac
     5221 aatcaaaaca ccgcaaaaac ggatgttaat ggtctggcga cttttgatct gaaaagtagt
     5281 aagcaggaag acaacacggt tgaagtcacc cttgaaaatg gcgtgaaaca aacgttaatc
     5341 gtcagttttg tcggcgactc gagtaccgcg caggttgatc tgcagaagtc gaaaaatgaa
     5401 gtggttgctg acggcaatga cagcgccaca atgaccgcga ccgtccggga tgcaaaaggc
     5461 aacctgctca atgacgtcat ggtcactttc aatgttaatt cagcagaggc gaaactgagc
     5521 caaaccgaag tgaatagcca cgacgggatc gccacagcta cgctgaccag tttgaaaaat
     5581 ggtgattata gggttacggc ctctgtgagc tctggttccc aggctaatca acaggtgaat
     5641 tttatcggtg atcaaagtac tgctgccctg accctcagtg tgccttcagg tgatatcacc
     5701 gtcaccaaca cagctccgca acatatgact gcaaccttgc aggataaaaa tggcaaccca
     5761 ctaaaagata aagaaatcac cttctctgta ccaaacgacg tcgcaagtcg gttctcgatt
     5821 agcaacagcg gaaaaggcat gacggatagc aacggggttg caatcgcctc cctgaccggc
     5881 acgttagcgg gcacgcatat gatcacggct cgtctggcta acagcaatgt cagcgataca
     5941 cagccaatga cgtttgtggc ggataaagac agagcggttg tcgttctgca aacatcgaaa
     6001 gcggaaatca ttgggaatgg cgtggatgag acaactctga cagcaacagt gaaagatcct
     6061 tttgataacg tggttaaaaa tctgtcagta gtcttccgca cctcccccgc agacacgcaa
     6121 ctgagtctga aggcgcttaa tactaatgag aacggtattg ctgaagttac ccttaagggc
     6181 acggttttgg gtgtttatac agccgaagcc atactgctta acggtaaaag cgatacgaaa
     6241 atcgtcaata ttgtgcccga taccagcaac gcgcaggtca ccctgaacat cccagctcaa
     6301 caggtggtga cgaataacag cgacagcgtg cagctgacgg cgacggtgaa agatccgtcg
     6361 aatcatccgg tggcgggaat aacggtgaac ttcaccatgc ctcaggacgt ggcggcaaac
     6421 tttacccttg aaaataacgg tattgccatc actcaggcca atggcgaagc gcatgtcacc
     6481 ctcaaaggta aaaaagcggg catgcatacg gttaccgcaa cgttgggtaa caataatgcc
     6541 agcgatgcgc aacctgtcac attcgtggcg gataaggaca gcgcagttgt cgttctgcaa
     6601 acatcgaaag cggaaatcat tgggaatggc gtggatgaga cgactctaac agcaacagtg
     6661 aaagatcctt ttgataacgc agtaaaagat ctacaggtta ccttcagtac caagccggca
     6721 gatactcaac ttagtcagag cacaagcaat actaacgaca gtggtgttgc tgaagttacc
     6781 cttaagggca cggttttggg cgttcataca gtcgaagcca cacttcctaa cggcaacggc
     6841 tatacaacga cagtcaatat tgcgcccgat accagcaacg cgcaggttac gctgaacatc
     6901 cctgctcaac aggtggtgac gaataacagt gacagcgtgc agcttacggc gatggtgaaa
     6961 gatccgtcga atcatccggt ggcggggata acggtcaact tcaccatgcc acaggacgtt
     7021 gcggcaaact ttaccctcga aaataacggc attgccatca cccaagccaa tggggaagcg
     7081 catgtcacgc tgaaaggtaa aaaagcgggt acgcatacgg ttaccgcaac gctgggtaat
     7141 aacaatacca gtgattcgca gccggtaacg tttgtggcgg acaaaacctc ggctcaggtt
     7201 gtcctgcaga tgtcaaaaga tgagatcaca ggtaatggcg tcgataacgc aacgctaact
     7261 gcaacggtta aagatcagtt cgacaatggg gtgaataatc ttccggtaac attcagctca
     7321 gcctcttcag gactcaccct gaccccggga gtaagtaata ccaatgagtc tggcatcgcg
     7381 caggccactc tcgcaggcgt tgcctttggt gagaagacgg ttactgcatc actggctaat
     7441 aatggtgcca gcgacaacaa aactgtgcat tttattggcg acacagcggc ggcaaaaatt
     7501 atcgagttga cgcctgtccc agacagcata atcgccggta ccccgcagaa cagctccggc
     7561 agcgtcatca ccgccacagt cgttgataat aatggctttc cggtgaaagg tgtgactgtg
     7621 aacttcacca gcaacgcagc gacagccgaa atgacgaacg gcggtcaagc cgtgacgaac
     7681 gaacagggta aggctaccgt cacttatacc aatacccgct cctcgataga atcaggagcg
     7741 agaccggata ccgttgaggc cagtctggaa aatggtagct ccacgcttag cacatcaatt
     7801 aatgtcaacg ctgatgcgtc tacggcacat ctcaccttgc tacaggcact ttttgataca
     7861 gtctccgcag gcgagacaac cagtctgtat attgaggtga aggataatta cggcaacggt
     7921 gtcccccagc aggaggtaac cctcagcgtt tcaccaagtg aaggcgtgac ccccagtaat
     7981 aacgctatat atactaccaa ccacgacggc aatttttacg caagctttac cgctacaaaa
     8041 gccggggttt atcaattgac ggcaaccctc gaaaatggcg attcgatgca acaaacagtg
     8101 acctatgtgc cgaacgtcgc gaatgctgaa atcaagctgg cagcctcgaa ggatccggtg
     8161 attgccgaca ataacgatct cacgacacta acagcaacag tcgctgatac agagggcaat
     8221 gcgatagcca acactgaggt aacatttact ctgccggaag atgtgaaggc gaacttcacg
     8281 ctgagcgatg gcggtaaagc gattactgat gctgaaggca aagcgaaagt cacgctgaaa
     8341 ggtacaaaag caggcgctca tactgttaca gcatcgatga ctggcggtaa gagtgagcag
     8401 ttggtggtga actttattgc ggatacgctc actgcgcagg ttaatcttaa cgttaccgag
     8461 gacaatttta tcgctaataa cgtcgggatg accaggctgc aggcaacagt gactgatgga
     8521 aacggcaacc cgttagccaa tgaggcggtg acattcacgc taccggcaga tgtgagcgca
     8581 agctttactc tcggacaagg cggttccgcc attactgata tcaacggcaa ggct
//
LOCUS       DAJTNN010000171         8599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTNN010000171 DAJTNN010000000
VERSION     DAJTNN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.171"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..190
                     /locus_tag="OO150_003454"
     CDS             <1..190
                     /locus_tag="OO150_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517474.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="OO150_003455"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="OO150_003455"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5517475.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="OO150_003456"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="OO150_003456"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5517476.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO150_003457"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO150_003457"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5517477.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /gene="dgkA"
                     /locus_tag="OO150_003458"
     CDS             4544..4912
                     /gene="dgkA"
                     /locus_tag="OO150_003458"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5517478.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="OO150_003459"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="OO150_003459"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5517479.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5703..7028
                     /gene="dinF"
                     /locus_tag="OO150_003460"
     CDS             5703..7028
                     /gene="dinF"
                     /locus_tag="OO150_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5517480.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7144..7353
                     /locus_tag="OO150_003461"
     CDS             7144..7353
                     /locus_tag="OO150_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5517481.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO150_003462"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO150_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5517482.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8228..8599
                     /locus_tag="OO150_003463"
                     /pseudo
     CDS             8228..8599
                     /locus_tag="OO150_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
BASE COUNT         2025 a         2044 c         2306 g         2224 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattct acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcact ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttatgtt gccttgtcgt
     8041 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8101 tcgctattta tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt
     8161 ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag
     8221 atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc
     8281 aaatcaattc gataatgaag atgcaatcaa agataaatta gataatagag gttcaatcaa
     8341 aggtaaatta aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga
     8401 tataattatt gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc
     8461 acgtaaaaaa atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag
     8521 tatattgacg attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt
     8581 gaccgggcta tttttcatt
//
LOCUS       DAJTNN010000172         8598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTNN010000172 DAJTNN010000000
VERSION     DAJTNN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.172"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..183
                     /locus_tag="OO150_003464"
     CDS             <1..183
                     /locus_tag="OO150_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5517483.1"
                     /translation="EAIKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQ
                     KGEVVGVDDQCKEHKE"
     gene            402..644
                     /locus_tag="OO150_003465"
     CDS             402..644
                     /locus_tag="OO150_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517484.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="OO150_003466"
     CDS             complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="OO150_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5517485.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="OO150_003467"
     CDS             complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="OO150_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5517486.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2532..3434)
                     /gene="fdoH"
                     /locus_tag="OO150_003468"
     CDS             complement(2532..3434)
                     /gene="fdoH"
                     /locus_tag="OO150_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5517487.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="OO150_003469"
     CDS             complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="OO150_003469"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5910..5912),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5517488.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVANYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6691..7524
                     /gene="fdhD"
                     /locus_tag="OO150_003470"
     CDS             6691..7524
                     /gene="fdhD"
                     /locus_tag="OO150_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5517489.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7678..>8598
                     /locus_tag="OO150_003471"
     CDS             7678..>8598
                     /locus_tag="OO150_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCR5517490.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYVDWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKIFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPY"
BASE COUNT         2049 a         2175 c         2273 g         2101 t
ORIGIN      
        1 gaggcaatta aacggctgaa caagcttcgg ggctggcgta aggtttcacg atctgcaatt
       61 ttacgcgaag cggttgagca atatctggag cggcagcaat ttccggtgcg taaagcaaag
      121 ggaggcaggc aaaaggggga ggtagttggt gttgatgatc agtgtaagga gcataaggaa
      181 tgaaaaaaat ttttcattct tgaatataaa aaaacagatg cccttattct ggtattaata
      241 caaggctgtt ttacttgaac ttataataac tgcaactgtt acatcgtatc tggaaaacgc
      301 ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact
      361 tttctctcca tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta
      421 tagatatggg cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc
      481 tcgaggtgca gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc
      541 tgataaatca atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg
      601 aagatggtgt cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt
      661 caatcagatt gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc
      721 cggcctacaa agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc
      781 agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt
      841 tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa
      901 tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg
      961 aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg gaactgcggg
     1021 catagccttc ttgctccatt cgcgcgtcca gtaccagaga agcgaggtca tctgccacgg
     1081 cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc
     1141 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
     1201 tctgttcaca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc
     1261 agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
     1321 aaccacaaac cgggcaatat tgacgttgct cgccgtattc agcgcgggct ttgccgggga
     1381 tcagattggc catctgcgcc cagtagagcg acagtgcagc ccagataaac ggcgctttat
     1441 cgctgctgac ggatgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
     1501 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
     1561 tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
     1621 gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca
     1681 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
     1741 gcaggtaatc atccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt
     1801 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     1861 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     1921 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac
     1981 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     2041 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     2101 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     2161 cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     2221 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     2281 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     2341 aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat
     2401 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgctaggat
     2461 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt
     2521 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     2581 tccgcacggt tcggaccgac acccacgtag tggaagatac tggctgcgaa ggtagccgca
     2641 aagccaacag ctgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     2701 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac
     2761 ataacgtgtg taccaccgac gcctgccgga tcgtacaggc ccgcattgtc gtaaccgcgg
     2821 gttttcagct ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     2881 tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg
     2941 tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg
     3001 aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg
     3061 ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag acagcctgga
     3121 tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc
     3181 acttccgaga agcgcatcac cgtccacgat ttggcactta aatcattggg gttgtcgtac
     3241 accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga
     3301 caggctttac agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag
     3361 tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttga
     3421 gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta
     3481 aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaaagtatt ggcaataaag
     3541 ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct
     3601 ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag
     3661 ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc
     3721 gactccccga tttccacaaa ctgctctggc tgcaaaattg cgttcaacag cgcgtgtttg
     3781 gtccagtagt ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg
     3841 gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagccgggtt cgagataacg
     3901 tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac
     3961 ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata
     4021 aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc
     4081 cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga
     4141 tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc
     4201 agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc
     4261 ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat
     4321 tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg
     4381 gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga cttcggctca
     4441 tgcggaatgg cgtagttcca ggtcatgttc agcacctgat ccgggttcgc gccaccctgt
     4501 tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcacc gtcagtcagc
     4561 gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg
     4621 attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc
     4681 ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg
     4741 ttcagcgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt
     4801 ttgttcggga atgaggcaac agggttaaaa ccctggcaga tatagccatt gaccttgccc
     4861 tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag
     4921 tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag
     4981 acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc
     5041 gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa
     5101 cctggcaggc tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg
     5161 cgcagggcgt taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc
     5221 gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg
     5281 aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac
     5341 gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc
     5401 tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag
     5461 ccgttttcgt ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca
     5521 tagccggtga aaaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg
     5581 gtataggctt cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg
     5641 cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtt agctaccgcc
     5701 gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg aattttggct
     5761 tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg
     5821 acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt
     5881 ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc
     5941 atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa
     6001 gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca
     6061 atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc
     6121 caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg
     6181 ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg
     6241 accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg
     6301 tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg
     6361 gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca
     6421 aaacctagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc
     6481 cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc
     6541 gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc
     6601 cagaattggc tcgatttccc tccatccagg aagggtttgt aacagaatac cataatgttg
     6661 gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa
     6721 aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa
     6781 cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt
     6841 tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg
     6901 ctttccgagg ggattatcga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc
     6961 tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag
     7021 cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat
     7081 gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg
     7141 gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact
     7201 cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt
     7261 cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag
     7321 caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg
     7381 tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct
     7441 gagcgctgta atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc
     7501 catccgcagc gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg
     7561 acttattcat ttcgtgaatt ttattatttt tatttataaa taaaacgcgt gtagtggcgt
     7621 tttctttttt catttattat gttcactctc ttatctatat atttataagg caattaaatg
     7681 aaaaggaatt tattatcttc cgctattata gtcgccatca tggccctcgg tctgacgggt
     7741 tgtgatgata aaaaagccga aacagaaacg cccccgcctg ccaatagtca acctgccgca
     7801 ccagctcctg aagctcccgt tgcaaaagca gaagctaaac ctgaaacacc tgcgcaaccg
     7861 gtggtcgatg aacaagcggt tttcgacgaa aaaatggatg tctatatcaa gtgctataac
     7921 aagttacaga tccctgtaca gcgcagtctg gcgcgttatg ttgactggct gaaagatttt
     7981 aaacaggggc ctaccggtga agagcgtact gtttatggca tctacggcat tagtgaatcc
     8041 aacctcgctg agtgtgaaaa aggcgtaaaa agtgctgtgg cgttaacgcc tgcgctgcaa
     8101 ccaattgatg gcgtggcggt gagttatatc gatgctgccg tggcgttggg taataccatt
     8161 aacgaaatgg ataaatatta cacccaagaa aattataaag acgatgcgtt tgcgaaaggg
     8221 aaaacgctgc accagacatt cttaaaaaat ctggaagcct ttgaacctgt agcagaatct
     8281 tatcatgcgg cgattcagga aattaacgat aagcgccagc ttgcggaact gaaaaatatt
     8341 gaagaaagag aagggaaaat attccactac tattctctgg cagtcatgat ttcagcgaaa
     8401 caaattaata acctgatatc gcaagagaag tttgatgttg atgcggcaat gaagaaagtg
     8461 tctgaactgg aaacgctggt ggcgcaggcc aaagaagcgg ataaaggcgg catgaatttc
     8521 tcgtttatta attcggcagg ccagtatcaa ctcgaggcta aaaaatacgt tcgccgcatc
     8581 agagataaag tcccgtac
//
LOCUS       DAJTNN010000173         8452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTNN010000173 DAJTNN010000000
VERSION     DAJTNN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.173"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO150_003472"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO150_003472"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5517491.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKHWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO150_003473"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO150_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5517492.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGGAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="OO150_003474"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="OO150_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5517493.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO150_003475"
     CDS             complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO150_003475"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5517494.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLVAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO150_003476"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO150_003476"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5517495.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7823..8137)
                     /locus_tag="OO150_003477"
     CDS             complement(7823..8137)
                     /locus_tag="OO150_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5517496.1"
                     /translation="MNVQTFFKLSYARQFFFPFIAIAGSTAQGGVIHFYGQIVEPACD
                     VSTQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIE
                     YN"
     gene            complement(8254..>8452)
                     /gene="csgC"
                     /locus_tag="OO150_003478"
     CDS             complement(8254..>8452)
                     /gene="csgC"
                     /locus_tag="OO150_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5517497.1"
                     /translation="LREGGSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVV
                     TVSDGQALHLSQQWPPSSEKS"
BASE COUNT         2106 a         2210 c         2002 g         2134 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg cggcgtgaac gccttaccca
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctctccgg ggaattccag
      241 acacggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgcg gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acaccgcgtg cataaaacca tcatcaaggg agcgttgacg attcatctca
      481 aggaagcgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccagt gtttggttcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaaccct aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcacccc aggaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaaa gcacttccag tagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atagtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgtactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttcacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggtaat
     1261 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacaaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaac ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca ggcgcaccag cccgcacaaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactaagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca ggccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccattaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaagaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacgacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccgacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt cagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca agcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     2581 gcatccagcg cctgatgtac ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 accccactcc cctttcggag tcacccatgc gcttggacga agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccgaagcctt gcgggttctc
     3301 catcgagaag ctgctaaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattaccg gcatggatag agagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caccccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaagg cggtatcaat tgccaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catatcgcgg aaaacggagg gcaagttgct
     4081 tttgggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaattt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat tatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgctggt atttttcgtt
     4801 atatccggct acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccttact gacattgccg
     4921 caatttatta tgctgcaata cgtcaaaggg aaagcggaaa gttggcctgg gctgtcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgcgtatgg atatttaaac gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc gcccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttgcc gttctttatc
     5281 ctcggtgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc ttgcagcagc attggcattt gtcgcttatt tactcaacca acgctatggc
     5401 agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggtctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttactttg ttaacgcatc gctatttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tttctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg ggttcttttt tgagaataat ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcatactggc
     5941 tctgtataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtc gaacgtggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaggtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgttttcgc ccagataagc ggcaacgttc
     6481 catcaaccag atgatttata aacggactgg attccatttg gcgtaagtaa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacgtc ctctaccacg ggtcctattg ccataacatc taaatccgaa aaaagtggct
     6721 cttcccctgc cccaaaatag gcatcaccaa tatttcgtcc tcccaccaag gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatac ggcgattgag acgggaaaag tcggtgatat
     6841 aaccaagcgg acgcagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcggg
     6901 gatggctgtc aagcaagcgt aaaatatcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cgactaacag ggcggaaaac agtaaccgac
     7021 ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt ttttactgcg
     7321 atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc aggaaatgcc
     7381 actgacgtat agctgtttgc cgccaccagt cgcagactat tgagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg tactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttaa
     7741 caccttcttg tttcttaagt gtacagcagg ctttttggtt aaatcgcatt tttctactca
     7801 gcaacgtgcg ttgttcagaa acttagttat attcgatgtt cattacagcc agctttttct
     7861 gtttatcaat atactgcact tttactgagg ctacttgcgc gtttttgaca ttgccgctca
     7921 ttaacgcttt gctggaatag gtttttcccg gaacagaacc attctgtggg cagttcattt
     7981 ctacgggtga tgactgggtg ctgacgtcac atgccggttc cacaatttgg ccataaaaat
     8041 ggatcacgcc cccttgcgcg gtgcttcctg cgatggcaat aaaaggaaaa aaaaactgcc
     8101 gagcataaga gagtttaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg acatttcaac aaattaagac ttttctgaag agggcggcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaata ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaaccg ccttcgcgca gg
//
LOCUS       DAJTNN010000174         8414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTNN010000174 DAJTNN010000000
VERSION     DAJTNN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.174"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            27..638
                     /gene="ruvA"
                     /locus_tag="OO150_003479"
     CDS             27..638
                     /gene="ruvA"
                     /locus_tag="OO150_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5517498.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            647..1657
                     /gene="ruvB"
                     /locus_tag="OO150_003480"
     CDS             647..1657
                     /gene="ruvB"
                     /locus_tag="OO150_003480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5517499.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1707..2492)
                     /gene="znuB"
                     /locus_tag="OO150_003481"
     CDS             complement(1707..2492)
                     /gene="znuB"
                     /locus_tag="OO150_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5517500.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLALDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2489..3244)
                     /gene="znuC"
                     /locus_tag="OO150_003482"
     CDS             complement(2489..3244)
                     /gene="znuC"
                     /locus_tag="OO150_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5517501.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3323..4255
                     /gene="znuA"
                     /locus_tag="OO150_003483"
     CDS             3323..4255
                     /gene="znuA"
                     /locus_tag="OO150_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5517502.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4271..5593
                     /gene="mepM"
                     /locus_tag="OO150_003484"
     CDS             4271..5593
                     /gene="mepM"
                     /locus_tag="OO150_003484"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5517503.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5713..6684
                     /gene="lpxM"
                     /locus_tag="OO150_003485"
                     /gene_synonym="msbB"
     CDS             5713..6684
                     /gene="lpxM"
                     /locus_tag="OO150_003485"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5517504.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRRPGEIQPYKRKDLYPIK"
     gene            complement(6773..>8414)
                     /locus_tag="OO150_003486"
     CDS             complement(6773..>8414)
                     /locus_tag="OO150_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5517505.1"
                     /translation="IDGLPHVDFSRVDKFFTYADAFRESLPIISGELYFEAHQGCFTS
                     ESATKAHNRNMENKLHDAEFFTTITNNMTSILRSEFDEIWKEMLTLQFHDILPGSCIS
                     RVYLETEKEYIKLEAKTEKIISDTQSTLLSKIDTSSYKDPHILFNTTCFARNEWININ
                     NNWLKARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSENGDLISLYDKRYGK
                     EYITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIY
                     HYNSSYLRFAFTLTQDSPLVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVID
                     RPTHSDDSFAFAKDEIPAHHWVDLSDRETGIALIAKNKYGYRVKDQTLKLTLLRSQHK
                     PGEVVKTDNYDNFLINNFADIGKQKFIFSLFPHHGDYRVGGVINQAYIMNHPLQVVPV
                     KPHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESHGLEISAMLSWVCNYH
                     YVQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2279 a         1953 c         2088 g         2094 t
ORIGIN      
        1 tttgattcat tacgcaggag cgtcatgtga taggcagact cagaggcatc atcattgaaa
       61 aacaaccccc gctggtgtta attgaagtgg gcggcgtagg ctatgaagtg catatgccga
      121 tgacctgttt ttatgaactc cctgaagcgg gtcaggaagc gatcgttttc acccactttg
      181 tggtgcgtga agacgcgcaa ctgctgtacg gttttaacaa taaacaagag cgcacattgt
      241 tcaaagagtt gatcaaaacc aacggcgtcg gcccgaagtt ggcgctggcg atcctctccg
      301 gaatgtcagc gcagcagttc gttaatgccg ttgaacgtga agaagtgggg gcactggtga
      361 aactgccggg tattggcaaa aaaaccgccg aacgcttgat tgttgaaatg aaagaccgat
      421 ttaaaggttt gcatggcgat ctgtttaccc ctgccgccga cctggtactc acatcacctg
      481 ccagcccggc gaccgacgat gctgaacagg aagcggttgc cgcgctggtg gcgctgggct
      541 ataaaccaca agaagcaagc cgcatggtga gcaaaatcgc tcgccctgac gccagcagtg
      601 aaacattaat tcgcgaagcc ctacgcgccg cgttatgagg taaaggatga ttgaagcaga
      661 ccgtctgatt tctgccggta ccactttgcc ggaagatgta gcagatcgcg ccattcgccc
      721 taaattactg gaagagtatg ttggtcagcc gcaggttcgt tcacagatgg agattttcat
      781 caaagcagcg aaactgcgcg gcgatgccct cgatcatttg ttgatttttg gtcctccggg
      841 gttgggtaaa actacgcttg ccaacattgt cgccaatgaa atgggcgtta atttacgcac
      901 gacttctggt ccggtgctgg aaaaggcggg cgatctggca gcgatgctca ctaaccttga
      961 accgcatgac gtgctgttta ttgatgagat ccaccgttta tcgccagttg ttgaagaagt
     1021 gctgtacccg gcaatggaag actaccaact ggatatcatg attggtgaag gtccggcggc
     1081 acgctccatt aaaattgatt tgccgccgtt taccctgatt ggcgcaacca cgcgcgcagg
     1141 ttcgctgaca tcaccgttgc gcgaccgttt tggtattgtg caacgtctgg agttttatca
     1201 ggtgccggat ctgcaatata tcgttagtcg cagtgcacgc tttatggggc ttgagatgag
     1261 tgatgacggc gcgctggaag ttgctcgtcg cgctcgcggt acgccacgta ttgccaaccg
     1321 tctgctgcgt cgagtgcgtg atttcgccga agtgaagcac gatggcacca tctcggcaga
     1381 tatcgctgct caggcgctgg acatgttgaa tgtcgatgct gaaggtttcg attatatgga
     1441 ccgcaaattg ttgctggcgg taatcgataa gttctttggt gggccggtag gtctggataa
     1501 cctggcggcg gctattggcg aagaacgtga aaccattgag gatgtgctgg aaccttattt
     1561 gattcagcaa ggctttttgc agcgtacacc gcgtgggcgt atggcgacga cgcgggcgtg
     1621 gaatcacttt ggcataacgc cgccagaaat gccgtaagtc ggattggtcg ctcgcgccgc
     1681 atccgacaaa tgtgttcagc gatagattag ctggcctgct ttttcatcat actgataata
     1741 aataacagtg ctgcacatag caccaccgac ggacccgccg gcgtatcgta aaatgcggaa
     1801 aaggttaaac cgcccgttac ggcaaccatc cccaccaaca cagcgacacc tgccatctgt
     1861 tccggcgtgc gggcaaagcg acgtgcagta gcagcaggaa taatcagcag tgaagtaata
     1921 atcaacgcgc caacaaattt catcgctaca ccaatcgtca atgccgtcac cagcatcaac
     1981 aacaatttca cgcgctgtaa tttcacacca tcaacaaacg ccagatccgg gctaatcgtc
     2041 atcgacagca aattgcgcca ttgccagaac aaaatagcca ccacgatgac cacgccaatc
     2101 gcaatagcga tgagatcttc tggcgtcact gccagcaaat caccgaacag gtaagccatc
     2161 aaatcaacac gaatgttaga catcagacta acgaccacca ggcccagcga cagggcactg
     2221 tgcgccataa tccctaataa cgtgtcaagc gccagctgtg gacgtttctc cagccatacc
     2281 agaccgcccg ccaggagcag cgtaacggca attaccgcat agaatggatt cacgtccagc
     2341 aacaaaccaa acgcgacgcc aagtaatgag gcatgagcca gcgtatcacc gaaataagac
     2401 atacgacgcc agactacaaa cgaacccagc ggacccgcgg cacaggcgag catgatcccg
     2461 gctaaccaac cgggaaataa taattcaatc atgagcgatc atttccccga cgcaaaacaa
     2521 ttcgtccctg taaatcgtga cgatgattat gatgatggcg atagataccc agttgttcag
     2581 caccacgagg accaaacata gaaataaact ccggatgtag ggaaacaact tccggtgtgc
     2641 cggaacaaca aatgtggtga ttcagacaca gcacttcatc ggtttttgcc attaccagat
     2701 gcagatcgtg agagaccatt aaaacgccac aatccagttc gcgacgcaac tggtcaataa
     2761 ggtcatataa cgccacctgg ccatttacat ccacgccttg agtgggttca tccagcacta
     2821 ataattgcgg tcgatttaac aatgctcgcg ctaacagtac acgctgtgtt tcgccaccag
     2881 agagcttttg catcggtgcg ttaatcagat gcccggcctg gacacgtttc agtgcaggca
     2941 aaatatcttc tttatgtgtg ccagggcgta agcgtaaaaa acggtttacg gtcagtggca
     3001 acgtggtgtc gagatacagc ttctgcggta catagccgat gcgtagtttt ccgttgcgct
     3061 tgataacccc ttcatcgggt gttaccagcc cgagcactac ccgtaccagt gtcgacttac
     3121 ctgcgccatt tggcccaagt aaagtcaaaa tttttccagg tttaagttcc agcgacacat
     3181 cagagaggac gcggcgttgg ccaaaagaaa ccgagacatt ttccagggaa accagacttg
     3241 tcatgttaat tttagtcttg cagtagtcat gagatgttat aatatcacac ttctcatatt
     3301 cattacgatg attggtcgca ttatgttaca taaaaaaacg cttcttttcg cagcattatc
     3361 cgccgctctc tggggaggtg caacacaggc cgcagatgcc gccgttgtcg cttcgcttaa
     3421 acccgttggg ttcatcgctt ctgccattgc tgatggtgta acagaaacgg aggttttact
     3481 gcctgacggc gcttcagaac atgattattc actgcgtcca tcggatgtaa aacgcttaca
     3541 gaacgcggac ttagtcgttt gggttggccc ggagatggaa gcgtttatgc aaaaaccggt
     3601 aagcaaatta ctaggagcga agcaggtaac gattgcgcag cttgaagatg tgaaactgct
     3661 actgatgaaa agtattcacg gcgatgatga tgatcacgac cacgcggaaa aaagtgaaga
     3721 agatcaccat cacggcgatt tcaacatgca tctttggctt tccccagaga tagcgcgggc
     3781 tacagcggtt gcaatccatg gaaaattagt ggaacttatg ccgcaaagtc gagccaaact
     3841 tgacgccaac ctgaaggatt ttgaggcaca attagcctca accgaaacgc aggttggtaa
     3901 cgagctcgcg ccgctcaagg ggaaaggtta tttcgttttt cacgatgctt acggctattt
     3961 cgaaaaacag ttcggactga caccgcttgg tcattttacc gttaaccctg agattcaacc
     4021 tggcgcgcag cgtttacatg aaataagaac acagttggtt gagcaaaaag caacctgcgt
     4081 ttttgctgag ccacagttca ggccagcggt cgttgagagc gtcgcacgag ggacatccgt
     4141 tcgtatggga acgttggatc ccttggggac gaatatcaaa ctgggtaaaa caagctattc
     4201 agaattcctg agtcaattag ccaaccagta tgcgagctgc ctgaaaggag attaatgagg
     4261 aagtgattac gtgcaacaga tagcccgctc tgtcgccctg gcgtttaata atttaccgcg
     4321 accacaccgc gttatgttgg ggtcgctcac cgttcttact ctggccgtcg ctgtctggcg
     4381 gccctatgtt tatcaccgcg atgccacgcc aattgtcaaa accattgagc tggaacagaa
     4441 cgaaattcgt tcgctcttac ctgaagccag tgagccgatt gatcaagctg cacaagaaga
     4501 tgaagccatt ccccaggacg aactggatga caaaatcgcc ggtgaagcgg gcgtgcatga
     4561 atatgttgtt tccactggcg atacgctaag cagcattctc aatcagtatg gtattgatat
     4621 gggtgatatc acccaactgg ctgcggccga caaagaattg cgtaacctga aaatcggtca
     4681 acaactctcc tggacattaa ccgcggacgg cgaactgcag cgcctcacct gggaagtgtc
     4741 tcgtcgtgaa acccgaacct atgaccgtac tgccgctaac ggttttaaaa tgaccagcga
     4801 aatgcagcaa ggagagtggg ttaacaatct gctgaaaggt accgtcggag ggagctttgt
     4861 tgccagcgcc agaaacgccg gtttaaccag cgccgaagtg agcgcagtga ttaaagccat
     4921 gcagtggcaa atggatttcc gcaaactgaa aaaaggcgat gaatttgcgg tgttaatgtc
     4981 tcgagagatg cttgatggta aacgtgagca aagccagctg ctgggcgtac gtttgcgttc
     5041 agaaggtaaa gattattacg caatccgcgc tgaggatggc aaattctacg accgtaacgg
     5101 tactggtctg gcgaaaggat tcttgcgatt cccaacggcg aaacagttcc gtatctcgtc
     5161 taactttaac ccgcgtcgta ctaatccggt gaccggtcgc gttgcaccac acagaggtgt
     5221 tgatttcgcc atgccgcagg gtacgccagt gctttcagtg ggtgacggtg aagtggtggt
     5281 tgccaaacgc agtggcgcag caggttatta tgtggctatt cgtcatggtc gcagctacac
     5341 cacgcgttat atgcacttgc gcaagattct ggtgaaaccg ggacagaagg tgaaacgtgg
     5401 cgaccgtatc gcgctttccg gtaataccgg acgttcaacc gggccgcatc tgcactatga
     5461 agtatggata aaccagcagg ccgtaaaccc gctgacggca aaactgccgc gtaccgaagg
     5521 gctgaccggc tccgatcgtc gcgaattcct ggcgcaggcc aaagagattg tgccgcagct
     5581 acggtttgat taattaacat ccattcgcag ccggtacgca gtcagtaccg gcttttttta
     5641 tttggtgcgg ggcaagttgc gccgctacac tatcaccaga ttgatttttg ccttatccga
     5701 aactggaaaa gcatggaaac gaaaaaaaat aatagcgaat acattcctga gtttgataaa
     5761 tcctttcgcc acccgcgcta ctggggagca tggctgggcg tagcagcgat ggtgggtatc
     5821 gctttaacgc cgccaaagtt ccgtgatccc attctggcac ggctgggacg ttttgccgga
     5881 cgactgggaa aaagctcacg ccgtcgtgcg ttaatcaatc tgtcgctctg ctttccagaa
     5941 cgtagtgaag ctgaacgcga agcgattgtt gatgagatgt ttgccaccgc gccgcaagcg
     6001 atggcaatga tggctgagtt ggcaatacgc ggaccggaga aaattcagcc gcgcgttgac
     6061 tggcaagggc tgcagatcat cgaagagatg aggcgtaata acgagaaagt tatctttctg
     6121 gtgccgcacg gttgggccgt cgatattcct gccatgctga tggcctcgca agggcagaaa
     6181 atggcagcga tgttccataa tcagggcaac ccggtttttg attatgtctg gaacacggtg
     6241 cgtcgtcgct ttggcggtcg tctgcatgcg agaaatgacg gtattaaacc attcatccag
     6301 tcggtacgtc aggggtactg gggatattat ttacccgatc aggatcatgg cccagagcac
     6361 agcgaatttg tggatttctt tgccacctat aaagcgacgt tgcccgcgat tggtcgtttg
     6421 atgaaagtgt gccgtgcgcg cgttgtaccg ctgtttccga tttatgatgg caagacgcat
     6481 cgtctgacga ttcaggtgcg cccaccgatg gatgatctgt tagaggcgga tgatcatacg
     6541 attgcgcggc ggatgaatga agaagtcgag atttttgttg gtccgcgacc agaacaatac
     6601 acctggatac taaaattgct gaaaactcgc agaccgggcg aaatccaacc gtataagcgt
     6661 aaagatcttt atcctatcaa ataaaaaaaa gcctctcaaa ttattgagag gctgtactcg
     6721 ataaaaaatc tcaatgagtg ttcccactat tttgagtttt taggttagta cgctattgag
     6781 ttaatcgtag cgtgataatt tcaaaaggtt tgaactcaag attgcaatat tgattgcaat
     6841 aaaaactgtc atctaactta ttttcttgaa gatcgacata ttgaacgtag tgataattac
     6901 atacccacga aagcatggct gaaatttcta atccatgact ctcatataat cttacgatta
     6961 taccgtcgcc gtcttcagct aattttattg tttcgatgat aacgctgtta gtatcgatag
     7021 caaaaaatga tagtgaaggg ggtaattcac ccgggtgcgg tttgacaggg acgacctgta
     7081 agggatgatt cattatatag gcttgattaa taacaccacc cacacggtaa tctccatgat
     7141 gaggaaataa ggagaatatg aatttttgtt taccaatatc tgcaaaatta ttaatcagga
     7201 aattatcata attgtctgtc ttaacaactt ccccaggttt atgctgcgaa cgcagcaaag
     7261 ttaatttaag cgtctggtct ttgacacgat agccatattt gttttttgct attaaagcta
     7321 tgccagtttc tcgatcagaa aggtcgaccc agtgatgtgc tggtatttca tctttggcaa
     7381 aagcgaacga atcatcgcta tgcgttggtc tgtcaattac accgaactga atttgacatt
     7441 gtgcaagaga tgtttgtact gacaccggga atttgacttt gagagaaaca ttaggttcgt
     7501 gccaatcaat aaaagtttgg acatcaacga gtggactgtc ctgtgtcagg gtaaatgcga
     7561 agcgaagata agaggaattg tagtgataaa tcagcgtcat cgtagtttta ggcccgctga
     7621 ttacagttgt cattttttca gcgagtaaaa catatgattc cccatcacga taatttgaag
     7681 caaaatccca ggccgcgaaa aaacctgcat cttcgtggta tgcacggatc tcactgtgca
     7741 tattttcagt aatgtattct ttaccatatc ttttatcata tagcgaaatt aaatcaccgt
     7801 tttctgaaaa aagaagttta atgtaattat tttcaatact tcgagattct gccttcagac
     7861 catttacgat cttattcttt ggatcaatca cagcataacc ataagagttt actcgtgctt
     7921 tgagccagtt gttgtttata ttgatccatt cgttacgagc aaaacaggtg gtattaaata
     7981 aaatgtgcgg atctttataa gaagaagtat caattttact taataaagta gactgcgtat
     8041 cagaaataat tttctcggtt tttgcttcta gtttaatata ttccttttca gtttcaagat
     8101 aaactcttga aatacatgat ccgggaagga tgtcatggaa ttgcaaggtc agcatctctt
     8161 tccatatttc atcaaactca ctgcgaagaa ttgatgtcat attattagta atagttgtaa
     8221 aaaattcagc atcatgtaat ttattttcca tatttcgatt atgagcttta gttgctgact
     8281 ctgatgtaaa gcacccttga tgagcctcga aataaagttc acctgaaata atgggcaagc
     8341 tttcgcgaaa agcgtcggcg taagtgaaga acttatcaac acggctgaaa tcaacatgag
     8401 gtaagccatc aata
//
LOCUS       DAJTNN010000175         8162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTNN010000175 DAJTNN010000000
VERSION     DAJTNN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.175"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..783)
                     /locus_tag="OO150_003487"
     CDS             complement(<1..783)
                     /locus_tag="OO150_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5517506.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            875..961
                     /locus_tag="OO150_003488"
                     /pseudo
     CDS             875..961
                     /locus_tag="OO150_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1262..1351
                     /locus_tag="OO150_003489"
                     /pseudo
     CDS             1262..1351
                     /locus_tag="OO150_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            1375..5889
                     /locus_tag="OO150_003490"
                     /pseudo
     CDS             1375..5889
                     /locus_tag="OO150_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            5890..7539
                     /locus_tag="OO150_003491"
     CDS             5890..7539
                     /locus_tag="OO150_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5517507.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIHTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            7544..>8162
                     /locus_tag="OO150_003492"
     CDS             7544..>8162
                     /locus_tag="OO150_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517508.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQV"
BASE COUNT         1994 a         1964 c         2289 g         1915 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggt aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatgtt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgctcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct
      901 ggccgaaaat gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg
      961 ttggtcgccc taccttttca tcaagccagt agtctgccca ccactgcatc atttctctgc
     1021 gcttatcgag atactgagca tggttgtaaa ttcaactaac ccagtgcgtt gacaaaaaat
     1081 tagcgcaaga agacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca
     1141 gctaagtagt tgattcatag tgactgcata tgttgtgctt tgtcgcattt tgtagtctat
     1201 tatttaatct gtaacaacga taatacaatg aattttaatt ggtttttgtg attttgtcct
     1261 tcttctgaca tggaaggaca cccgatggat acccaacgat tccaatccca atttcattgg
     1321 catttatcgt ttaaattttc tggcgcgata gcagcgtgct tgtctctgtc tcttgtggga
     1381 acagggcttg ctaatgctga tgattcgctt ccttccagta actatgcgcc gcccgccggg
     1441 ggaacattct ttttgcttgc tgacagcagt tttagcagca gtgaagaggc gaaagtgcga
     1501 ctggaagcgc cggggcgtga ttatcggcgc tatcagatgg aagagtacgg cggcgtggac
     1561 gttcgcctgt atcgtattcc tgacccgatg gcatttttgc gccagcagaa aaacctgcat
     1621 cgcattgtgg tgcaaccgca atatctgggc gacgggctga acaatacgct gacctggctg
     1681 tgggataact ggtacggcaa atctcgccgc gtaatgcagc gtactttctc ttctcagtca
     1741 cggcagaatg tgactcaggc attacccgaa ttacatctcg gcaatgccat tattaaacct
     1801 tcccgttatg tacagaacaa ccagttttcc ccgctgaaaa aatatccctt ggtggaacag
     1861 ttccgttatc cactatggca ggctaaaccg gtcgagccgc agcaaggggt aaaactggaa
     1921 ggcgcatcca gcaatttcat ctcgccgcag ccgggtaaca tttatattcc tctcggccaa
     1981 caagagccgg gactgtacct cgtcgaggcg atggttggtg ggtatcgggc gacgacggtg
     2041 gtgtttgttt ccgataccgt ggctcttagc aaagtgtcag gcaacgagct tctggtgtgg
     2101 accgcgggta aaaaacaggg tgaagcgaag cccggctcag agatcttgtg gactgacggt
     2161 cttggcgtga tgacccgcgg tgtgaccgat gacagcggta ccttgcagtt acaacatata
     2221 tcgccagaac gttcatacat tctgggtaag gatgctgaag gcggcgtttt tgtctccgag
     2281 aacttcttct acgaaagcga aatctacaac acccgcttgt atatttttac cgatcgcccg
     2341 ctatatcgcg caggcgatcg tgtcgatgtt aaagtgatgg gccgcgagtt ccacgatccg
     2401 ttgcattcat cccccatcgt cagcgccccg gcgaagcttt cggtgctgga cgctaacggc
     2461 agtctgttgc aaaccgtcga tgtcacgctg gatgcgcgca atggcgggca gggaagtttc
     2521 cgcctgccag aaaatgccgt tgccggaggt tatgagttac gtcttgctta ccgcaatcag
     2581 gtctatagca gcagttttcg cgtggcaaac tacatcaagc cacatttcga gattggttta
     2641 gctctcgaca aaaaagagtt caaaactggc gaagcggtca gcggcaaact gcaactgctc
     2701 tacccggatg gcgagccggt aaaaaatgcc cgcgtgcagt taagtttgcg cgctcagcaa
     2761 ttatcaatgg tcggtaacga tttgcgttat gccggacgtt tccccgtgtc gctggaaggc
     2821 agcgaaacgg tgtccgacgc cagcggtcat gtggcgttaa atctccccgc cgccgataaa
     2881 ccgagccgtt atttgttaac cgtctccgcc agtgacggcg cggcgtatcg tgtcaccacc
     2941 accaaagaga tcctcattga acgcggtctg gcgcattact cattaagtac tgccgcacaa
     3001 tacagtaata gcggcgagtc ggttgtgttc cgttatgccg cgctggaatc ttcaaaacag
     3061 gttcctgtta cgtatgaatg gttgcgtctc gaagaccgca cgagccatag cggagagcta
     3121 ccgtcaggcg gcaaatcctt taccgtcaat ttcgctaaac ctggcaacta caatctgaca
     3181 ttacgcgata aagacggctt aattctcgct gggttaagtt atgccgtcag cggtaagggc
     3241 agcacggcgc atacgggtac ggtagatatc gtggcggata aaacgctgta ccagccaggc
     3301 gaaaccgcga agatgctgat tacctttccg gagccaattg atgaagcatt attgacgctg
     3361 gaacgcgatc gcgtggaaca gcagtcgctg cttttgcatc cggcaaactg gctaacgcta
     3421 caacgtttaa acgataccca gtatgaagcc cgggttccag tgagcaattc ctttgcgcct
     3481 aacatcactt tttcggtgct gtatacccgt aatggtcagt acagttttca gaacgccggg
     3541 atcaaagttg ccgttcctca gcttgatatc cgggtgaaaa cggacaaaac ccattaccag
     3601 ccaggtgaac tggtcaatgt cgaattaacc tcgtcgctga aaggtaaacc tgtttctgcg
     3661 cagctaacgg taggcgtggt cgatgaaatg atctacgcgc tgcaaccaga aatcgcgccg
     3721 aatatcggca aatttttcta tccgctgggg cgtaacaatg tgcgtaccag ctccagtctg
     3781 tcgtttatca gctacgacca ggcgctctcc agcgagccgg ttgcgcctgg cgcaactaac
     3841 cgcagcgagc ggcgagtaaa aatgcttgaa cgtccacggc gtgaagaggt ggataccgcg
     3901 gcatggatgc cgtcactcac aaccgataaa caaggcaaag cgtatttcac gttcctgatg
     3961 cctgattcgt taacccgctg gcgtatcacc gcgcgtggga tgaacggcga cgggctggtc
     4021 gggcaggggc gtgcttatct gcgttcggaa aaaaatctct acatgaagtg gagtatgcca
     4081 acggtgtatc gcgtgggcga caaaccggcg gcaggactgt ttatcttcag tcagcaggat
     4141 aacgaaccgg tggcgctggt gactaaattt gcaggcgctg agatgcgcca gacgctgacg
     4201 ctgcacaaag gggcgaatta tatttcgctg gcgcagaata ttcagcaatc tggcttgtta
     4261 agtgcagaac tgcaacaaaa tgggcaagtg caggacagca ttagcacaaa actgtctttt
     4321 gtggacaaca gcaggcccgt tgaacagcag aaaaatgtca tgctcggcgg tggcgagaac
     4381 gcgctgatgt tgcccgagca ggcgagtaat atccggctac aaagtagtga aacgccgcag
     4441 gagattttcc gcaacaatct tgatgcgtta gtcgatgaac cgtggggtgg ggtgatcaac
     4501 accggtagcc gtctgatccc gctcagtctc gcctggcgtt cgcttgccga tcatcaaagt
     4561 gccgccgcta acgacattcg tcagatgatt caggataacc gtctgcggct gatgcaactg
     4621 gcggggcccg gagcgcgctt tacctggtgg ggtgaagatg gcaatggtga cgccttcctt
     4681 acggcatggg catggtacgc cgactggcag gccagctagg cgctcggcgt aacgcaacaa
     4741 ccggaatact ggcagcatat gctcgacagt tatgccgagc aggcggataa catgccgtta
     4801 ttgcatcggg cgctggtgct ggcgtgggca caggagatga atctgccgtg caaaacgttg
     4861 ttgaaagggt tggatgaagc tatcgcccgg cgcggaacta aagatgaaga tttctctgag
     4921 gaagacatcc gcgatatcaa tgacagcctg atcctcgata caccggaatc tccactggca
     4981 gatgcggtgg caaacgtctt aaccatgacg ttgctgaaaa aagcgcagtt gaagtccacg
     5041 gtgatgccac aggttcagca atatgcgtgg gataaagcgg caaacagcaa tcagccgctg
     5101 gcgcacacgg ttgtgctgct caatagcggt ggtgacgcta cccagacggc cgctatttta
     5161 agtggtttga ccgctgagca atccactatt gagcgcgcgc tggccatgaa ctggctggcg
     5221 aaatatatgg cgacaatgcc tccagttgtt ttgcctgcgc ctgcgggcgc atgggctaaa
     5281 cataagttaa ctggaggggg cgaatactgg cgttgggttg gtcagggcgt gccggacatt
     5341 ctctcttttg gtgacgaatt atcgccgcaa aatgtgcagg tccgctggcg tgagccggca
     5401 aaaatggctc aacaaagtaa cattccggtg accgttgaac gccagttgta tcggcttatc
     5461 cctggtgaag aagagatgag ctttattctg caaccggtga ccagcaatga gattgacagc
     5521 gatgcgctgt atctcgatga catcacgctt accagcgagc aggatgcagt tctgcgctac
     5581 ggtcaggtgg aagtaccgct gccaccggga gccgacgttg agcgcacaac atggggcatt
     5641 tcggtcaata aacccaacgc cgcgaaacag caggggcaat tgctggaaaa agcgcgaaat
     5701 gaaatgggcg aactggccta tatggtgccg gtgaaagaac tgacgggaac ggtcactttc
     5761 cgccatttgc tgcgcttctc gcaaaaaggg caattcgttc tgcctcctgc tcgttatgtg
     5821 cgttcctatg cacctgcaca gcaaagtgtt gcggcaggga gtgaatggac cgggatgcag
     5881 gtgaaataag tgaactggcg cagaatcgtc tggctattgg cgttggttac tcagccaacg
     5941 cttgcggagg agacgccgtt acaactggca ctgcgtggtg cgcagcacga tcaactgtat
     6001 cagctgtcgt catcaggcgt gaccacagtc tcagcattac ctgactcgct caccacgccg
     6061 ttagggagtt tgtggaaact gtatgtttac gcctggctgg aggataccca tcaaccggag
     6121 cagccctacc agtgtcgcgg aaactccccg gaagaggttt attgttgtca ggctggggag
     6181 agcataacgc gcgataccgc gctggtacgt tcctgtgggc tctattttgc gccgcagcgg
     6241 ttgcatattg gcgcagatgt gtgggggcaa tactggcaac agcgtcaggc gcctgcgtgg
     6301 ttagcgtctc ttactacgct gaagccagaa acctcggtaa cggttaaatc gctgcttgat
     6361 tcattagcga cgttacctgc gcaaaacaaa gctcaggaag tcttactgga tgtggtgctg
     6421 gacgaggcga aaatcggtgt cgcatccatg ctcggtagtc gggtacgggt gaaaacctgg
     6481 agctggtttg ccgatgataa acaggaaatc cgtcagggtg gttttgccgg ctggttaacc
     6541 gatggtacac cgctgtgggt tactggcagt ggcaccagca aaacggtact aacccgttat
     6601 gcgacagtgc ttaatcgggt tttgccagtg ccgacgcagg ttgccagtgg gcaatgtgta
     6661 gaggtggagc tgtttgcccg ctatccgctg aagaaaatca cagcggaaaa gagtacgacg
     6721 gcagtaaaac ccggcgtact taatgggcgc tatcgtgtga cgtttactaa tggtaaccac
     6781 attacgtttg tcagccacgg tgagacgacc ttactgagcg aaaagggcaa actgaaattg
     6841 cagtcgcatc tcgatcgtga agagtacgtt gccagagttc tggatcgcga ggcgaaaagt
     6901 acgccccctg aggcagcgaa agccatgacc gtggcgattc acactttttt acagcaaaac
     6961 gccaatcggg aaggcgattg cctgaccatc cccgacagca gcgccacgca gcgtgtttct
     7021 gcttcgccag cgaccacggg ggcgcgaacg atgaccgcct ggacgcagga tctcatctat
     7081 gcaggcgatc cggttcatta tcatggcagt cgagctaccg aagggacact ttcctggcgg
     7141 caagccacgg cccaggcggg gcagggagag cgttacgacc agatactcgc gtttgcttat
     7201 cccgacaaca gccttagccg ctggggagcg ccacgctcaa cctgccagtt attacccaaa
     7261 gcgaaagcct ggctggcgaa aaaaatgccg cagtggcggc gtatattaca aggcgagacg
     7321 gggtacaacg aaccagacgt gtttgcggtc tgtcgtttag tctcaggttt cccctatacc
     7381 gatcggcagc agaaacggct gtttattcgt aacttcttta cgcttcagga tcggctggat
     7441 ttaacccatg agtatctgca cctggccttt gatggttatc ccaccgggct tgatgagaac
     7501 tatatcgaaa cgctgacccg acaattatta atggactaat gctatgcgaa agatttttct
     7561 tccgttgtta ctggtggcac tttcgcctgt cgctcacagt gaaggtgtgc aggaagtcga
     7621 gattgacgca ccgctctctg gctggcatcc ggcagagggc gaagatgcca gtttttcaca
     7681 atcgatcaat tacccggcgt cgtccgtcaa catggcagat gatcaaaata tctcagcgca
     7741 gatccgcggc aaaataaaga attatgctgc ggcgggtaaa attcagcagg gccggctggt
     7801 ggtcaacggt gccagtatgc cgcagagagt tgaatccgat ggttcatttg cacgccctta
     7861 tattttcact gaaggcagca acagcgtgca ggtcatcagc ccggatgggc aaagccgaca
     7921 aaaaatgcag ttttactcaa cgccgggtac aggaacgatt cgtgcacgtt tacggctggt
     7981 tctctcgtgg gatacggaca ataccgatct cgatctccat gttgtcacgc ccgatggcga
     8041 acacgcctgg tacggtaaca ccgtgctgaa aaacagtggt gcactggata tggatgtcac
     8101 gacggggtac ggacccgaga ttttcgccat gccagcgcca gttcacggac gctatcaggt
     8161 gt
//
LOCUS       DAJTNN010000176         8071 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTNN010000176 DAJTNN010000000
VERSION     DAJTNN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.176"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..260
                     /locus_tag="OO150_003493"
     CDS             <1..260
                     /locus_tag="OO150_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5517509.1"
                     /translation="VVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRF
                     YRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(399..1028)
                     /locus_tag="OO150_003494"
     CDS             complement(399..1028)
                     /locus_tag="OO150_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5517510.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1151..2797)
                     /gene="fumB"
                     /locus_tag="OO150_003495"
     CDS             complement(1151..2797)
                     /gene="fumB"
                     /locus_tag="OO150_003495"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5517511.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGIAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDTCHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2875..4215)
                     /gene="dcuB"
                     /locus_tag="OO150_003496"
     CDS             complement(2875..4215)
                     /gene="dcuB"
                     /locus_tag="OO150_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5517512.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="OO150_003497"
     CDS             complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="OO150_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5517513.1"
                     /translation="MSNVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="OO150_003498"
     CDS             complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="OO150_003498"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5517514.1"
                     /translation="MRHSLPYRMLLKRPMKLSTTVILMVSAVQFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVVSGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYSLKTANNYKEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7314..7544
                     /locus_tag="OO150_003499"
     CDS             7314..7544
                     /locus_tag="OO150_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR5517515.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7556..7828
                     /locus_tag="OO150_003500"
     CDS             7556..7828
                     /locus_tag="OO150_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5517516.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT         2006 a         1957 c         2073 g         2035 t
ORIGIN      
        1 cggttgtgtt agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat
       61 taggtatgca gtttattatg attgctctac caactctgtt tttcatggta acgctgattc
      121 tctacttccg tttctatcgc ctcaatggtg acacgctgcg caggatccag atccatctgc
      181 tggataaata tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag
      241 tgagtgatgt gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt
      301 ttatcaggcc acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg
      361 aattcacgct gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag
      421 cgccgtagct ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc
      481 agcgcgcaga acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca
      541 tccgctccct gacccattgc cagcgccgcc caccactcga ttaattcata cattgcgctt
      601 atcgccagcg ccacgcagca gactaggaac gccaccattt tacgtccgcg cacgtacatc
      661 ccgcgaacga gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaagtgcccc
      721 agcttgtcat acggattacg gctcaacccc agccattcct gcacctcaaa accaacgggg
      781 actttcgcgt aggtgtattg tccgccgacc atcaggatga tggcgtgaag gaaaatgagc
      841 gtatagagca gcggcgttaa cggataacgt ctggcggtgg caagcagtag cggcacgaca
      901 ataatcaccg gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta
      961 taaatcagga ttaatgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg
     1021 cgggtcatgg tgagttcgca aatcagggaa aatactgact attcaccagc aagcgtgaaa
     1081 ttacaatcgc aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg
     1141 gccgaagagg ttacttagtg cagttcgcgc actgtttgtt gacgatttgc tggaagaagt
     1201 cgttaccttt gtcatcgacc aagataaacg ccgggaaatc ttctacttcg attttccaga
     1261 tagcttccat acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg
     1321 ccagtaccgc cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg
     1381 tgtcggtaac ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg
     1441 attgcagcag atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg
     1501 atggataacc ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt
     1561 actgcggaag ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc
     1621 ggcccacgat aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct
     1681 gggcgaggat ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg
     1741 cctggcgcag ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga
     1801 taccttcgcg gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc
     1861 cgaccgggca ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt
     1921 atttaccgcc aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt
     1981 ccagctggac atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt
     2041 aatagtgagc gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa
     2101 tcacaaacgc gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga
     2161 cgaggaagtt tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg
     2221 ttttgttggc agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta
     2281 ccgcgtacag gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt
     2341 ccagcgccgc attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt
     2401 tcgacagcgc ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga
     2461 tgatcgcgat gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggaat
     2521 ttcttaagaa ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa
     2581 tagccgcaac ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt
     2641 gctgcgccag cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt
     2701 cggcaacgct aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg
     2761 ggaaaggtgc ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta
     2821 tttgttattc gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa
     2881 gaacccgtac atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa
     2941 ccccggcaga ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg
     3001 gtcaaactga atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca
     3061 agccggtgct gaagccacga tgtatgccgg atcaacgccg atcgccaacg cgaccggaac
     3121 aatcgccgcc agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat
     3181 ggcataggcc cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat
     3241 atgcgcaccg aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat
     3301 cataccggaa cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat
     3361 aataatcagc gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg
     3421 tttgccgccg aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat
     3481 tgccccgagg aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatccagcag
     3541 cgtcgcggta tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa
     3601 ctcttcgtct ttatccagat ctttaccacg gaaccagctg aagataccga tcgccaggat
     3661 accgattaac gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc
     3721 atcaaaggtg acattaccca gcatcgcaac cagagacacg accgcaaccg acaccggact
     3781 ggcgataatc cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat
     3841 gttgttctta atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt
     3901 accgcaaaga atggtcagcg tacaggtcac aaacggcgcg acaattgaga catatttcgg
     3961 gttgcggcgc agcaacttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg
     4021 caaggtcgcc gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt
     4081 acctggctga aggtggaaga caaagaccag aatgaccaga ccgataccgc ctaataaacc
     4141 cagcgcgata cccccctttc tggcaccata aaacagacat atcagtatta tgataagttg
     4201 gatagtaaat aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga
     4261 tcacagtcac gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg
     4321 tcgttaccgg ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa
     4381 tttatgcact gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata
     4441 gtttgtactg cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc
     4501 tattttttgt tctcagggca gatatcatta ggtttaaaga ttaatcttat ctacccattt
     4561 gtgggtgaaa atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt
     4621 tgaaattaaa aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata
     4681 attatatttg atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt
     4741 ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg
     4801 tttcagtagt gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac
     4861 gccataatgg atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg
     4921 gcaggaaaca cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc
     4981 ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg
     5041 caagcgacga ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc
     5101 ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc
     5161 ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag
     5221 cgaatcttta atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca
     5281 acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc
     5341 gagcaatatc aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag
     5401 cgtcgaggct gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag
     5461 ctccgcgacc attgcgtcgt catcgataat taatacattg ctcatctgtt tgacctctcc
     5521 ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg
     5581 atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg
     5641 cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc
     5701 ggtccatcat cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta
     5761 atttcgccac cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat
     5821 gtggtaatca gcgtcgcgac ctggtcctca ctgccgctgt ctggcagcag gctttcactg
     5881 tttaaaatca gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca
     5941 gcgataaccg gagatttgat cttacccagc agagagccaa tctcttcctt atagttattg
     6001 gctgttttga gactgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga
     6061 atcacatgca atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg
     6121 accagaccgt cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa
     6181 atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg
     6241 tctttaatcg taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca
     6301 tctaccacct gtgaccatga gtggcttagc gtcgacagtt tctcatcgtc ctgcgactta
     6361 cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc
     6421 acggcaacga cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg
     6481 gagatttcgt agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag
     6541 gtgccaatca gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg
     6601 tcattgattt gttgggtcac gcggcttaac tcaaggccgc tcaccaccac gccaatttgt
     6661 ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga
     6721 ttgatagcga cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc
     6781 tgaccaatac gctgggcttc aggatgcgag tagcgaagac tttgcatatc cgtaacgaca
     6841 ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc
     6901 tgcggttttt tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact
     6961 gccagtgcct tgttggccag cccgtctcgt gtcatatcac tgatttgcga gaagtaaatc
     7021 agatgcacca ccaatagcac cgagaactgt accgcgctga ccattaagat cactgtggta
     7081 ctcaatttca tcggacgttt gagtaacatg cggtagggca atgaatgtct catcagcttc
     7141 cttgtgtgac aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa
     7201 gaatgattgg ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa
     7261 gattccaggg ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc
     7321 aggcgctact ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca
     7381 acactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc
     7441 tcaaacgaaa gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga
     7501 ttgatacgtg cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga
     7561 aatacgcgaa ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga
     7621 aattgtcttt gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga
     7681 aagcctgaaa gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg
     7741 tcgggaaaaa cgaaaaatta tcccactatg cccatttgcg aaacacgaat ttgataaaac
     7801 gcgggagtat gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg
     7861 ctctccgcgg agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc
     7921 agagagcgcg tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc
     7981 ctatatgaga atcatcaatc ggggttaata agttttgcgt ccccagagca ttcaatattg
     8041 ataggagtca tattatggaa ggtaaaaaca a
//
LOCUS       DAJTNN010000177         7910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTNN010000177 DAJTNN010000000
VERSION     DAJTNN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.179"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(123..953)
                     /locus_tag="OO150_003501"
     CDS             complement(123..953)
                     /locus_tag="OO150_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5517517.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO150_003502"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO150_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5517518.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO150_003503"
     CDS             2379..2729
                     /locus_tag="OO150_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5517519.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO150_003504"
     CDS             2855..3415
                     /locus_tag="OO150_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5517520.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO150_003505"
     CDS             3418..6384
                     /locus_tag="OO150_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5517521.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO150_003506"
     CDS             6451..6828
                     /locus_tag="OO150_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5517522.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO150_003507"
     CDS             complement(7029..7688)
                     /locus_tag="OO150_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5517523.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2242 c         2133 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
//
LOCUS       DAJTNN010000178         7854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTNN010000178 DAJTNN010000000
VERSION     DAJTNN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.180"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(497..859)
                     /locus_tag="OO150_003508"
     CDS             complement(497..859)
                     /locus_tag="OO150_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5517524.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            1144..1353
                     /locus_tag="OO150_003509"
     CDS             1144..1353
                     /locus_tag="OO150_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517525.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1365..1952)
                     /gene="mtlR"
                     /locus_tag="OO150_003510"
     CDS             complement(1365..1952)
                     /gene="mtlR"
                     /locus_tag="OO150_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5517526.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1952..3100)
                     /gene="mtlD"
                     /locus_tag="OO150_003511"
     CDS             complement(1952..3100)
                     /gene="mtlD"
                     /locus_tag="OO150_003511"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5517527.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(3330..5243)
                     /gene="mtlA"
                     /locus_tag="OO150_003512"
     CDS             complement(3330..5243)
                     /gene="mtlA"
                     /locus_tag="OO150_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5517528.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLT
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5780..6142
                     /locus_tag="OO150_003513"
     CDS             5780..6142
                     /locus_tag="OO150_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5517529.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6145..7281
                     /locus_tag="OO150_003514"
     CDS             6145..7281
                     /locus_tag="OO150_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5517530.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGGLQSLTVVPGTDGVQGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1962 a         1908 c         1903 g         2081 t
ORIGIN      
        1 tgtccattgt attattggtg atttatgttg ctttacatta atgacagtga tgtgtgtcga
       61 ttaaatattg ttttaagcat tcatcataaa aatgtatcat tcgtttcaaa agatgtctgt
      121 gtttgtatga atatattatt ttcgtggatt ctaatgatga ttattaataa atgtcaaata
      181 tgaaagaaac catgtctggg aaaaaattta cagataattt attgtatgaa tatgatgatt
      241 aaaatttcac aattaaatcc gaaaaaaaga gacgtaactg cttgttaatt aataaatgat
      301 atcttctata tatttttatt gtttataggt ggctatttcg gtaaaaatgc attttcagaa
      361 tatcaaccag cggtaaatcg acgtaaaggg agtgaattgt ttggtaatta ggcgagtaga
      421 caggagagtc cccgcattga ccagctaagg caagccagat gcgggttgat atgatcgaac
      481 cagtcgtctg aggcgcttaa aacgcagtct tagagaatcc agtcatctcc tgcaggccca
      541 tttcacggcc cagcgcggtc attgggtgca caaccaccag tccgcgaaca cttttcttca
      601 gcttgcccat atcagcctgc tcttttttgg taattgcgcg ctgaaacggc atcttcatca
      661 gcttctgtgc ttctttactc agtttttggc tatgaacctc acgcaggcga gcaatttctg
      721 cttccagcgt ggctttctct ttttccagct cggcatattt gtctgcggct tcaaccagcg
      781 acagatcggc ctgctggtgg cggatggcgt ccaggcgatc gctgagacgt ttgatttcgt
      841 ttttttcgac ttctttcatg tgatggcttc tgataaagat aaatttatag cccctagtat
      901 gcgcgaccag gtgctttgtt cacaactgat gacattgtaa tagttctgca acgtaatcat
      961 taacatgctc aaaaactacg caattcgttg attaattaag aaatctatag aaaaaagata
     1021 ataaacagca gcggatgatt attgaggatt cagcacgttt tccttaatgt catctttacc
     1081 gggaacctaa actaaaattg gtggccgcgc attcccggcg cggggtaata cggagatatc
     1141 atcatgggca aattaggtga aaacgttccg cttcttatcg ataaagccgt agatttcatg
     1201 gcatcaagcc aggcgttccg ggagtatctg aaaaaacttc ctccccgtaa cgcgattccg
     1261 tccggaatac ccgatgaaag cgtgccgtta tatctacaac gtctggagta ttatcgtcgg
     1321 ctttatcggc cgaagcaggt agaggggcag taagcagacg ttgcttactt ttgaaaggct
     1381 ttttttaagc tgattttgga aatcagctca gtcagtgaaa ggaccattgt tgatcgcaca
     1441 gcctgttgat agcgctgaat ttgcattgca tacaaactgg agtctgctgg ttcaaactgt
     1501 ggcggcggtg gtaacgccgc cacgcagtgc agttcaccaa agggtccaag gatttcgtcg
     1561 tcggtaaagg cgtactcgtt gccgtcgtga tttagctctt cacgcaatgc catcagcagt
     1621 tccgcatctt cgtattcctg gcggttaatg acgcccaacc cgtaaatgag ttttaaacgc
     1681 acagaaagat cgcccagcgg accgtcgccg tcgagtaacg gttctacagc atacttcacc
     1741 gcgtaatcgt ctttgcggaa tacctgaagc accagaagat ttaccgcctc ggtcaaaagc
     1801 tcgacggcgg tgatcagaaa gcttcgcacg gttttgccag cattcagacg ctcaagcaca
     1861 cggttttcaa aggcctgggt ttgttccatt gttgcctgca tatctgacaa tctgttattc
     1921 gggcgcaggg tgtcctgcgc ctggtccacc attattgcat tgctttataa gcggttaccg
     1981 cctcggatac aacctcactg ttggcatcaa gaccggaaat ctgtgccagc gccgcctgcg
     2041 gacctttgtc agcgatcagt gctgccagtt cctgagcctg cggatcatct tcactgcgga
     2101 agtgcattgc accagcaatc ccctgaatca gattcttgtg cggaaggctg tattccagcg
     2161 taccgagcag tggcttgatc agacggtcgc cagcactcag tttacgcagc ggctgacggc
     2221 ctacgcgctc tacatcatct ttcagatacg ggttctcaaa acgaccgagg attttctgga
     2281 tgtacgccgc atgtttgtct gcgtcaaagc cgtagcgctt gatcagtacc gcaccacttt
     2341 cttccatcgc accttttacc accgcgcgga ttttctcgtc gagaatagcg tcacgaatgg
     2401 tctgatgacc ggccagtttt ccgaggtacg cggttatagc atgacccgtg ttcagggtga
     2461 agagtttacg ttcgacaaat gccatcaggt tgtcggttaa ctccatgctt gggatgttcg
     2521 gcagtgcgcc tttgaactgc gttttatcga caatccattc gctgaaagtt tctaccgtca
     2581 cttccagcgg atcgttagtt gccgaagccg aaggcggtac gatgcggtca acggcggaat
     2641 cgacaaagcc aacgtgttct tctacccacg ctttggcgtc ttccggcagg gcgttcatca
     2701 catggccttt cagctgcgtg gtaccgcgta ccatgttttc acaggcgatg atgttcagcg
     2761 gggattcatt accttgttct ttgcgtttca ccagcccttt ggcgatagcc ggagcaatac
     2821 gttccagcac aaccgggcca acggcggtag tgactaaatc gacctgagca atcagatcaa
     2881 cgacatcatc accaatgctg ctgacagcat tgacgccgga aacggtatct acctgctcgg
     2941 tttcaccaac cacatgaacc tgatagctat gacgggcatt cagggcatca agtaccacct
     3001 gattgacatc ggcaaacgtc agttggatac ccgcgtctgc cagcagttta ccgataaagc
     3061 cacgaccgat attacctgcg ccaaaatgta atgctttcat agtattaacc ttcatcaatg
     3121 tttttacccg agagggctgg ggtgaggctt ttccctcacc caaaccctct ccgtgaggag
     3181 aggggcagcg ccggatgcgg cgtaagcgcc ttatccggcc tacaagaacg tgcaaattca
     3241 acaaatcgtg atgcatcctg taggcctgat aagcgtagcg catcaggcaa ttaaccccac
     3301 cttctccatg tggagagggt gggattggat tacttacgac ctgccaacag ttccagcact
     3361 tcatccacgc tggtggtgtg tgccagacgc tcgatgacgg actcatcatc cagtgcattg
     3421 gtcaggctgg tgataacctg aatgtgctcg ttgttacggg ctgcaatacc aatcaccaga
     3481 cgggcaatgt catcttcctc ttcaccgaag cgcacgcctt ccgggtactg gcagaacacc
     3541 acgcccgttt tcagtacgcg atctttcgct tcaaccgtac cgtgtggcac cgcgatagac
     3601 tcacccagat aagtcggggt cagtttttca cgatccagca tcgcctgaac gtattccggc
     3661 tcaacgtaac cgcctttcac cagctgctcg ccagcaaaac gaatcgcttc ttctttggtt
     3721 gctgctttgc gaccgaggaa gatgttctcc gcgcccagtt tgaacaggtt agcactggaa
     3781 tcgtcaaagc tgtctttcag actgtctttc actttcactt cgttttcagt atggcgctgg
     3841 gcagcaacca gacgttcggt taggctggtg tacaggccgc tgtcgaggaa gttggtcagc
     3901 gaaatatgct gtgcctgcgg aacctggcgc atagcgcgtt cggtcaggtc acggtgagtg
     3961 atgacgaggt ccacatctgg cggcaggttg ttgatcgcgc tgttagtgac cgaaatctgc
     4021 gacagacctg catcctgaat tttcttacgc agcacgcctg cgcccatcgc acttgaaccc
     4081 ataccggcat cacaggcaac gatgatttta cgtacgtggc tcaggtcgtt agtcacatcg
     4141 cccgcagaca gcggagatgt gcctttagac tcagctttca tgtcctgcat acgacgagtt
     4201 gctgcttcaa tatcatcttc ttctttcact ttgctggttt tcagcaaaat agcagagaca
     4261 acgaaggaga cagccatcgc cgcacacaca cccgcgatgt tagcgaaata agcccctttt
     4321 ggtgtcatcg ccagtacagc aaggatagaa cccggagatg ccggagaaac cagaccaccg
     4381 cccaggatag tcagcgtgaa cacgccagtc ataccgccga ggatgactgc gaggatcaga
     4441 cgcggattca tcagcacata cgggaagtag atttcgtgga taccacccag gaagtggatg
     4501 attgccgcac cgcccgcaga ctgtttagcg ctaccacgac caaagaacat gtacgccagc
     4561 agcacgccca tacctggacc tgggttagct tcaatcagga agaagattga tttacccagt
     4621 tcatgggact gctgaatacc cagcggcgag aagataccgt ggttaatggc gttgttgagg
     4681 aacaggattt tcgccggctc aacaaagata gacgccagcg gcagcatgtc atggacaacc
     4741 atgaagttaa cgcccgcagc cagcattttg gacagggctt caacaatcgg gccaatgccg
     4801 aggaatgcca gaatagcgag gatcatcccg atgatgcctg cggagaagtt attcaccagc
     4861 atctcaaaac cggatttgat cttaccgtct acccagcggt cgaagtgctt aatgcaccag
     4921 ccgcccagcg gacctgcaat catagaaccg aggaacatcg gcatgtctgc gccgacgata
     4981 acgcccatgg tggtgatggc accgactacg ccgccacgtt cgccgcctac cagcttacca
     5041 ccggtataac cgatcagcag cggcaggaga taagtgatca tcggcccgac cagcttcgcc
     5101 agcgtctcgt tcgttaacca ccctgttgga ataaataacg cggtgatgat accccacgcg
     5161 ataaacgcgc cgatatttgg catcaccatg ttgctgagga aacgaccaaa gctttgcact
     5221 ttgatcttaa tatcggatga cataaaaaca ccccttctta tgtttgctgt cgcgcaggct
     5281 ggaagcccga ggttttttgt taatgtggcg gcagaggtag ccggaccttg tagatactgc
     5341 gaaatctggc actgaatcgg ttaactgtcc agtcgacggc cttttgtgtg atattaatca
     5401 cataaatata ggaggtagag aggtaattga cgtgacgttc atcacaaaac gcttgtgtaa
     5461 aaaagcaaca aggcggcgat tttcgctaaa aatggcattt aaatgtgagt agtgtcacat
     5521 ttttgttttg atggtttgtt gaatctttgt gatctgaatc acaagatatt ttcttacggc
     5581 gttcacctga tgtgatctac agcatgttat gccttcgtca tacaatcgtt atgtaactaa
     5641 gcaactcatc acctctaaaa attattcttt ggtgttatgg cgagaatcgc gaacccggat
     5701 tattgtcaaa atatcagcct gactcccttc tggctgccag cccaaaaaca acactgataa
     5761 acgtgaatcc gaggcagata tgtttctaaa ctatttcgcg ttgggagtgc tgatcttcgt
     5821 ctttctggtg attttttatg gaatcatcgc gatacatgac atcccttatc tgattgccaa
     5881 aaagcgcaat cacccccatg ccgacgctat tcatacggcg ggctgggtga gcctgtttac
     5941 tctgcatgtt atctggccgt ttctgtggat ctgggcgacg ctctatcaac cggagcgtgg
     6001 ctggggtatg cagtcacatg ttgcgtcgca ggagaaagcg actgacccgg aaatcgcctc
     6061 actttctgac cgaatttccc ggctggagca tcaactcgcc gccgagaaaa agactgacta
     6121 ttccacgttc ccggagatct aattatggat ctattgattg ttttaactta cgtggcgctg
     6181 gcgtgggcgg tctttaaaat cttccgcatt ccggtaaatc agtggacgct ggcgacggcg
     6241 gcgctgggag gcgtgtttct ggtgagtggt ttgattttgt tgatgaacta caaccaccct
     6301 tacactttta ccgcgcaaaa ggcagtgata gcgatcccta tcacgccaca ggtgacggga
     6361 attgttactg aagtcactga caagaataat cagcttattc aaaagggcga ggtgcttttt
     6421 aagctcgacc cggttcgtta ccaggcgcga gttgacagac ttcaggctga cctgatgacg
     6481 gcgacgcata atataaagac gctgcgtgcg cagctcactg aagcgcaggc caacaccacc
     6541 caggtttcag cggagcgcga ccgtctgttt aaaaattatc aacgttactt gaaaggcagc
     6601 caggcggcgg tgaatccgtt ctcggaacgt gacatcgacg atgcgcggca aaatttcctc
     6661 gcgcaggatg cgctggtgaa aggctcggtg gcggagcagg cgcagatcca gagccagctc
     6721 gacagtatgg ttaacggcga gcaatcgcag attgtgagct taagagcgca acttactgaa
     6781 gcaaaatata accttgagca gactgtcatt cgcgcgccga gcaatggcta cgttactcag
     6841 gtactgatcc gcccaggtac atacgcagct gccttgccgc tgcgtccggt gatggtcttc
     6901 atccccgagc aaaaacggca aattgtcgcc caatttcggc aaaactcgct gttacgtctg
     6961 aaacccggcg atgatgcgga agtggtgttt aacgcgctac ctgggcaggt gtttcacggc
     7021 aaactgacta gtattttacc tgtcgtgcca ggcggttctt atcaggcgca ggggggattg
     7081 caatcattaa cggtcgtgcc cggcacggac ggtgtgcagg gaaccattga actggaccct
     7141 aacgatgata tcgatgcctt acccgacggc atctacgccc aggtggcggt ttactccgac
     7201 catttcagcc atgtttcggt gatgcggaaa gtgctgctaa gaatgaccag ctggatgcat
     7261 tatctttatt tggatcattg agggggaggg gggagattat ggaactttgc tccttgtaag
     7321 gactaattat ttttgcatat tacttcatta gctgcgatga tttttcttta atattcatta
     7381 tgttaataag ttctggatga gtgtgctctc attggatttt ttatccgtct tgttctgccg
     7441 caggatgaga ctatacatcg tcgagatcgc aatgatgata tgagttgttc ctcctgcgac
     7501 ggctctaatc aaaaaagagc aggagtgctt ttccatctat tgcgatgatg gctttattat
     7561 ctgaaggatg acagtcacat ttaaaaaata tttaatctat agatcacaat gtagggtcat
     7621 ggaatatatg tatacaacta ttttcttctt cacttttaat tatcgtattc attataaaac
     7681 taagttaaaa aaactcctgg agagtgagtt attaatatat ttttaaataa aattaagtta
     7741 tcattcatcg atgcatttta tagaatcatc aatggccata atagtactat taactctcaa
     7801 ttttatattt ttatcatcac tacaaatatt aactgaattt atatcaatga tttt
//
LOCUS       DAJTNN010000179         7809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTNN010000179 DAJTNN010000000
VERSION     DAJTNN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.181"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            259..402
                     /locus_tag="OO150_003515"
     CDS             259..402
                     /locus_tag="OO150_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5517531.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            415..624
                     /gene="cspE"
                     /locus_tag="OO150_003516"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             415..624
                     /gene="cspE"
                     /locus_tag="OO150_003516"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5517532.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            790..1599
                     /gene="rlmA"
                     /locus_tag="OO150_003517"
     CDS             790..1599
                     /gene="rlmA"
                     /locus_tag="OO150_003517"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5517533.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1596..2162)
                     /gene="mntP"
                     /locus_tag="OO150_003518"
     CDS             complement(1596..2162)
                     /gene="mntP"
                     /locus_tag="OO150_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5517534.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2590..3048)
                     /locus_tag="OO150_003519"
     CDS             complement(2590..3048)
                     /locus_tag="OO150_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5517535.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLERIYKLIVLTQ"
     gene            complement(3103..3954)
                     /gene="manZ"
                     /locus_tag="OO150_003520"
     CDS             complement(3103..3954)
                     /gene="manZ"
                     /locus_tag="OO150_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5517536.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3967..4767)
                     /gene="manY"
                     /locus_tag="OO150_003521"
     CDS             complement(3967..4767)
                     /gene="manY"
                     /locus_tag="OO150_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5517537.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4830..5801)
                     /gene="manX"
                     /locus_tag="OO150_003522"
     CDS             complement(4830..5801)
                     /gene="manX"
                     /locus_tag="OO150_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5517538.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6264..>7809
                     /gene="yoaE"
                     /locus_tag="OO150_003523"
     CDS             6264..>7809
                     /gene="yoaE"
                     /locus_tag="OO150_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5517539.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDVDNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDED"
BASE COUNT         1984 a         1911 c         1938 g         1976 t
ORIGIN      
        1 aataacttct cccactggcc tggaacaact gaacttattg aactatgtta gaaaatacgc
       61 cagtttaagt atctgcctga actggcaagg ttaagcacaa tgatatatcg gcgcgtattc
      121 cgttgcataa gtgtgcaaaa aaagtggaag acgtatcgag atttgtgcgt ctgatcgaga
      181 catgtttaaa aatggcttgc cataattaac gttgtatgtg ataacagatt tcgggttaaa
      241 cgaggtacag ttctgtttat gtgtggcatt ttcagtaaag aagtcctgag taaacacgtt
      301 gatgttgaat accgcttctc tgccgagcct tatattggtg cctcatgcag taatgtgtca
      361 gttttatcta tgttatgcct gcgggcgaag aaaacaatct aaggaatttt tcaaatggca
      421 aagattaaag gtcaggttaa gtggttcaac gagtctaaag gttttggctt cattactccg
      481 gctgatggca gcaaagacgt gttcgtacac ttctccgcta tccagggtaa tggcttcaaa
      541 actctggctg aaggtcagaa cgttgagttc gaaattcagg acggccagaa aggtccggca
      601 gctgttaacg taacagctat ctgatcgaat ccactgatct gaagtgtgaa tacgcttcaa
      661 tctcgctata aagcctcgtc gaatgcgagg ctttttacta tgctttatct tcgctcctgg
      721 cgttcggata tttgcccgcc gcgtgattcg cgttacactt gcggccttta gtatcctgcc
      781 ggagttgtca tgtctttttc ctgtccactt tgccatcagc ctctttcgcg tgaaaaaaac
      841 agctatatct gtccccagcg acatcagttt gatatggcga aagaagggta tgtcaatctg
      901 ctgcccgttc agcataaacg gtctcgtgat ccgggcgaca gcgcggaaat gatgcaagca
      961 cgccgcgcat tcttagatgc cggacattat cagccgctgc gtgatgcaat tgtcgcccaa
     1021 ctgagggaac ggcttgatga taaggccacg gcggtgctgg atattggctg tggtgaaggg
     1081 tattacacac acgcatttgc cgatgcgttg cccgaaatca ccacgtttgg tctggatgtt
     1141 tcgaaggtag cgataaaagc ggcggcgaaa cgctatccgc aggtcacttt ttgtgtcgct
     1201 tccagccacc gtttgccgtt ttccgatacc agtatggacg ccataatacg tatttacgcg
     1261 ccgtgtaaag cagaagaatt agcacgagta gtgaagcccg gcggctgggt cattactgcc
     1321 acgccgggac cgcgacattt gatggagctg aaggggctga tttacaatga agtacatctt
     1381 catgcacctc atgcagaaca actggaaggt tttacattac agcagagtgc ggagttgtgt
     1441 tatccgatgc gtcttcgcgg tgatgaagcc gtcgcattat tgcagatgac gccgtttgcc
     1501 tggcgtgcga agccagaagt ctggcaaaca ctggcagcaa aagaagtgtt cgactgccag
     1561 acggacttta atattcacct ctggcagcgt tcttattaac cgtggaagtg cgtccagagg
     1621 atctggacgc cgatgccgat cagtaccagc ccgccgagaa tttccgcttt tttcccaata
     1681 attgagccga taaagcgacc aaccatcatc cctaatgttg acataatcaa ggttgcacaa
     1741 ccaatggcca atgcggtcgc gataatgttg acctgcagga aagcaagacc aacacccaca
     1801 gccatggcat ccaggctggt ggcaatcgcg gtggttacca gtagccagaa accgtgtcga
     1861 cggcgcggct cttcatcttc atcatctgcg ccacgaaaac cctcaataat cattcgcccg
     1921 ccgaggaata tcagcagcac aaacgcaatc cagtggttcc attcaaggac aaaccggctg
     1981 gctaacatgc ccattcccca gccgatcagc ggcgtcaggg tttcgacggc accaaaaata
     2041 aggccggttc gcaatgcttc agaaaatttc ggtttatgga gggtggcacc tttaccgatt
     2101 gatgcagcaa atgcatccat cgacatacca aacgcaagaa gaacagtagc agtgatattc
     2161 atgacaatgt cctgaccggg gtatccataa aacacatcat cgcccccagc aaacatgcat
     2221 acatgaacaa caagccgtga tggttttcgc gttcatgcag gcatagcgat tgatgtgcat
     2281 atggtctcgc ctgactgaaa gtattcagtc cgtacgtgcc acgttttgca acgagtatgt
     2341 tgacacgtac atttccggaa tctggaaatc ggctactccc caaatgaggg cgcaacctta
     2401 acatattttg agaatataaa acaactgttg gaatatatta tttcattgct ttattgataa
     2461 cgaattcatt tagatttata tatcaatgaa acgttaaaat aaataacaaa taagggaaga
     2521 ggtaatatag cctaagctat gtctgattat taaaaataca ctaaacatag ctttggctaa
     2581 attcataact tattgagtta aaacgataag cttataaatt ctttccagat tgtcgatatt
     2641 tcgaacgcgg attaacagcc gacgctgttc taattgcatc accaacacgc catcttccga
     2701 caagttcatc gctttgattc ggctatattc aatccagaca ttggcgaaga aaaaaccttt
     2761 ttgtttaaag atgatcttcg gaacgcggat ccagaatata taaaaaccca tcagagccag
     2821 tgcgcttaat aaccatgtcg ttattaatgc accatgattc gtgacgttgt tatagataag
     2881 aatgacaatc aatccgacga agataacgct atcgatgcga ccacgccgga gcaaaggaat
     2941 tgccagcagg gtggggccgt tacggcgggg catgatgaac tgatcgtaga tcgcgaaggc
     3001 caggagtgcg gcgatgaaaa gaatcagtac caggtccgtg attgtcatta atcctccaga
     3061 taaaaaaacg gggccaaaag gccccggtag tgtacaacag tcttacagtc ccagcaggcc
     3121 gcaagcgtaa ccagcgatac cgatgacgaa gaagccaacg atgatccaca gcgggttaac
     3181 ttttttgcgc agtagccaca tacaagcaaa ggtcagcagc agtggtacca ggcctggcat
     3241 taactggtcc agaatagtct ggacagtagt aacgtgttct ttgcccgtct ggtcagtaat
     3301 gcgagagaca accagcggga tgttgacatg tgtccacttg ttaaccaatg cccccatgac
     3361 aaacaggccg aggatagacg ccccttccgt cagtttttgc aggaagccac cacccatatc
     3421 tttaacgata tcgatacctt tggagtaacc atacgctacg ccgtagtaac gggttgccag
     3481 acgcaccagg ttaaacagga tgaagaacag cagcggacct aacaggctgc cgctcatcgc
     3541 gataccggca cccagtgctg caaataccgg acgtacggtt ccccagaaga tcgggtcgcc
     3601 tacaccagcc agcggcccca tcaaaccgac tttgataccg ttgatagcac cgtcgtcgat
     3661 ctctgcgcca ttagcacgct gttcttccag cgccagggtt acgccgagaa tcggcgcagc
     3721 cacgaacggc tgggtgttaa agaactccag gtgacggcga atagcttgtt tacgagcttc
     3781 gttgttctca gggtagaggc gacgaattgc cggtaccata gagaagcaga aacccagtgc
     3841 ctgcatacgt tcgaagttcc atgaaccctg gaagaggtta gaacgcagga agacgccacg
     3901 aatatcactt tgagtgagtt ttttctcggt ggtagtttga gttgtatcaa ccatttcgct
     3961 cacctgttag tccagttcgt tatcgagatc gttgttacca gcagcctgag caggcgcacc
     4021 ggctacgcgg ttgtatttcg ggctaagttg gatgtagagc actgccataa cagtaccgat
     4081 cacacccaga gcaaccaggt taaagttggt gaatgctgcg gttacgaagc cgaggtagaa
     4141 gaacggcatc aggtagccag cacgcatcat gttgataacc atcgcataac caaccaccac
     4201 gatcatgcca ccggcgatat tcagaccatt ggtcaccact tccggaatcg cattcagcat
     4261 gttctgtact tcgctggtac caacagacag cgcaacgatg acggccggaa tagccacacg
     4321 cattgcttgc aggaacagag aagaaacgtg gatccaggaa atcgctgtca ggttgccgtt
     4381 atcagcagcc ttatccgcag cgtgctggaa agcaacggta atagtacgaa cgatgatggt
     4441 cagtacctgg cccgcagcgg ccagagggat tgccagtgcg atacctgcac caatgctctg
     4501 atgacctgcg ataaccagaa tggtagaaat gatagaagcc agagcggcgt caggcgcaac
     4561 tgcagcaccg atgttcatcc agcccagcgc gatcatttcc agcgtaccac cgataataat
     4621 accggttttc atatccccaa gaacgatacc caccagggta cacgcgatta gcggacggtg
     4681 aaactgaaat tcatcgagga ttgatcccat acctgcgata caggctacga taaataccag
     4741 cacaatttga agagtggtaa tctccattgt acttctcctg tttacgtaag acttaagtgt
     4801 gaaaactgag tgataatcaa cacaatacgt tacttatcga ttttgctgat cagatccatc
     4861 attttcagtt tcggatcggt ggaaacctta cggacttcca gctcaatacc gcgcgcattc
     4921 agtttcttga acgcctcgat atctttttca tcaaccgaaa ccgcgttatt cacctgggtt
     4981 ttaccctgac ggaatgccat accaccgacg ttaacagagg tgattttcac gccgccttca
     5041 acgagacgct ctacatctgt tgggttggta aataacagca ttacgcgttc gccagcatat
     5101 ttcgggttgt tgtagacgcg aatcattttg gcaacatcaa ctacgtgtgc tgttacgccc
     5161 ggaggtgcaa cctgggtgag cagtgtctta cgcacggtat ccgcagccac ttcatcacta
     5221 acaacaataa tacgggagac attggtttct ttggtccagc gggtggcgac ctgaccgtga
     5281 atcagacggt cgtcgatacg cgcaaggcca ataaccatgt agtcgtttgg ccccattggt
     5341 tttgccggag ttggagccgc ttttggtgct gcggcagcgg gtgctggcgc ggctttttca
     5401 accggtttgg ctttcagtgc tttcacacct tcacggcctg tttctactgc cagcgcaacc
     5461 agttcatcaa agcttgggtc atcatcacga gccattaacg tttccacgag cattggaatg
     5521 ttaacgcctg caatgacttc ataatgctct ttgtcgacga caatgcggct ggcagcattg
     5581 aacgggctgc ctccccatgt atcaacgaga aacagcacgc ctttagtggt gtcgagtttt
     5641 gccaactgag cgttgtactt ttcaatcagc gtttcggcat tttcacctgg aacgaaatcg
     5701 atccagccga cgttttcctg ctcgcctaac agcatttctg ccgttttaag caactgctct
     5761 gcagcccaac catgtgtgcc tataacaata gcaatggtca cttgctacct cctttattat
     5821 cgttaacacc tcaacgtgcc agatgtattt ttgaatcgtc gtccacaatc gaatcgattc
     5881 agataagggc tacaaccaaa aacccctgcg tttcgcgatt tattttagat atcgaaaaaa
     5941 ttattttatg tgatgaagat ccgtaattta actttcgatt agcagaaatt tcgaaaggta
     6001 aaatatcctt gtcacattcg tttgcaaagg aaggtaaatc tttgccaaat cagaggcgtc
     6061 tctgatatgt ttaactcccg tttaacaacc atggagtata gggcagtagc ccgcagtatg
     6121 gatcgtcacc gacgtcattt cagcatcagg ccttttaacg cctgcctttc tggcactcta
     6181 tgccgcacct ttcgtttgca ttttgtcgtt acgcctgcat tatttctggc gtcgaatagc
     6241 tattccttaa gcaggagctt gtcatggaat tcttaatgga cccctcaatt tgggcggggc
     6301 tactcacgct tgttgttctc gaaattgtgc tgggtatcga taacctggtc ttcatcgcca
     6361 ttcttgctga caaactgccg ccaaaacaac gcgataaagc gcgtttgctg gggttatcac
     6421 tggcgctgat tatgcgtctg gggctgctgt cgctgatttc atggatggtc acgctgacca
     6481 aaccgctatt taccgtcatg gatttctcct tctccggacg cgacctgatt atgttgttcg
     6541 gggggatatt cttgctgttc aaagcaacaa ccgaactgca tgaacggctg gaaaaccgcg
     6601 atcatgattc cggccacggt aaaggctacg ccagtttctg ggtggtcgtc acacagattg
     6661 tcatccttga cgccgtcttc tcgttggatg cggtaattac tgcagtaggg atggttaacc
     6721 atctgccggt gatgatggcg gcggtagtga ttgcgatggc ggttatgttg ctggcatcca
     6781 aaccgctgac gcgattcgtt aaccagcacc ccacggtggt ggtgctctgt ctgagcttcc
     6841 tgttaatgat tggtcttagt ctggtggcag aaggtttcgg tttccacatt ccgaaaggtt
     6901 acctgtatgc cgcgattggc ttctcgatca tcatcgaagt gtttaaccag attgcgcgtc
     6961 gcaactttat tcgccaccag tcgactttgc cgctgcgagc gcgtactgcc gatgccatcc
     7021 tgcgtttgat gggcgggaaa cgtcaggcca atgttcagca cgatgtcgat aacccgatgc
     7081 cgatcccgga aggtgcattt gccgaagaag aacgttacat gattaacggc gtactgacgc
     7141 tggcgtcgcg ttctctgcgc gggatcatga cgccgcgtgg tgaaataagc tgggttgacg
     7201 ctaatctcgg ggtcgatgaa atccgcgagc aactgctctc ttcaccgcac agtctgttcc
     7261 cggtatgtcg cggtgaactg gatgaaatca tcggtattgt acgtgctaaa gaactgctgg
     7321 tggcgctgga agagggcgtt gatgtggcgg cgattgcttc ggcgtctccg gcgattatcg
     7381 tcccggaaac cctcgatccg atcaacctgt tgggcgtgct gcgtcgtgct cgcgggagct
     7441 ttgttatcgt gaccaacgag tttggtgtgg tacaaggtct ggtcacgccg ctggatgtgc
     7501 tggaagccat tgcgggtgaa ttcccggacg ctgacgaaac gccggaaatc attactgatg
     7561 gtgacggctg gctggtaaaa ggcggtacag atttgcatgc cttgcagcag gcgcttgatg
     7621 ttgagcacct tgccgatgac gatgatatcg cgacggtcgc gggcctcgtg atctcggcaa
     7681 atggtcacat tccccgtgtg ggcgatgtga ttgatgtagg gccactgcat atcaccatca
     7741 ttgaagccaa tgattatcgt gttgatctgg ttcgcattgt taaagagcaa ccggcgcacg
     7801 atgaagatg
//
LOCUS       DAJTNN010000180         7745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTNN010000180 DAJTNN010000000
VERSION     DAJTNN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.182"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..695)
                     /gene="dnaQ"
                     /locus_tag="OO150_003524"
     CDS             complement(<1..695)
                     /gene="dnaQ"
                     /locus_tag="OO150_003524"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5517540.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLV"
     gene            760..1227
                     /gene="rnhA"
                     /locus_tag="OO150_003525"
     CDS             760..1227
                     /gene="rnhA"
                     /locus_tag="OO150_003525"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5517541.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1224..1946)
                     /locus_tag="OO150_003526"
     CDS             complement(1224..1946)
                     /locus_tag="OO150_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5517542.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            1980..2735
                     /gene="gloB"
                     /locus_tag="OO150_003527"
     CDS             1980..2735
                     /gene="gloB"
                     /locus_tag="OO150_003527"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5517543.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            2807..4165
                     /gene="mltD"
                     /locus_tag="OO150_003528"
     CDS             2807..4165
                     /gene="mltD"
                     /locus_tag="OO150_003528"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5517544.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4214..4810)
                     /locus_tag="OO150_003529"
                     /pseudo
     CDS             complement(4214..4810)
                     /locus_tag="OO150_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(4841..5641)
                     /locus_tag="OO150_003530"
     CDS             complement(4841..5641)
                     /locus_tag="OO150_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5517545.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            5882..6796
                     /locus_tag="OO150_003531"
     CDS             5882..6796
                     /locus_tag="OO150_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5517546.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(6793..7586)
                     /gene="dkgB"
                     /locus_tag="OO150_003532"
                     /pseudo
     CDS             complement(6793..7586)
                     /gene="dkgB"
                     /locus_tag="OO150_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
BASE COUNT         2076 a         1852 c         1887 g         1930 t
ORIGIN      
        1 accagatcaa gacgggcttc atgagctgca agctcttcat ctgtcgcaaa aacaacgcgt
       61 aacttacttg cctgacgtac aatgcgctga attgttgctt caccttgttg ctgctgtgtc
      121 tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg tcatcgccag ataaacttcc
      181 gcaaggatct gtgcatcgag taatgccccg tgtagcgttc gtttactgtt atctatttcg
      241 tagcgggcac ataacgcatc gaggctgttg cgcttaccag gaaacatttt cctcgccacc
      301 gcaaggctat cggtgacctt acagaaggta ttggtcttcg gaatatcgcg cttaagcaac
      361 gaaaactcgt agtccataaa gccgatatcg aacgctgcgt tatggatcac caactccgcg
      421 ccgcggatat agtccatgaa ctcatcggct acttcggcaa acgtgggctt atcgagcaaa
      481 aattcatcgg caataccatg tacgccaaag gcttccggat ccaccagccg atcgggtttg
      541 agataaacat ggaagttatt gcccgtcagg cgacggttca ccacttcaac ggcaccaatc
      601 tcaatgatct tgtggccttc atagtgcgca ccaatctggt tcataccggt ggtttcggta
      661 tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg tcatttatgt cagacttatc
      721 gttttacagt tcgattcaat tacaggaagt ctaccagaga tgcttaaaca ggtagaaatt
      781 ttcaccgatg gttcgtgtct gggcaatcca ggacctgggg gttacggcgc tattttacgc
      841 tatcgcggac gcgagaaaac ctttagcgca ggctacaccc gcaccaccaa caaccgtatg
      901 gagttgatgg ccgctattgt cgcgctggag gcgttaaaag aacattgcga agtcattttg
      961 agtaccgaca gccagtatgt ccgccagggt atcacccagt ggatccataa ctggaaaaaa
     1021 cgtggctgga aaaccgcaga caaaaaacca gtaaaaaatg tcgatctctg gcaacgtctt
     1081 gatgctgccc tagggcaaca tcaaatcaaa tgggaatggg ttaaaggcca tgccggacac
     1141 ccggaaaacg aacgctgtga tgaactggct cgtgctgcgg caatgaatcc cacactggaa
     1201 gatacgggct accaagtcga agtttaagct tgtggtttac gacattgccg ggtggctcca
     1261 accgcttggc gaattcgtgg cttgttttta ctctgtttca tcgggtttag cgttaaagga
     1321 atagtccgtt tccgggcaac aataagttgt aagcaaccaa gcgcaggaat atgcgcattc
     1381 aatagttttc ctccgtgttt gttccacggg agaacgtgga aacggctggc gtgcagcact
     1441 tcaaaattca acaaagagag ccagtccagt tgccgcatca gagtaaacat ccggctgtta
     1501 tagggcgagg tttttcgcaa taccggcaca agtttgcgta atcccattaa actaatggga
     1561 ttgaagccac taatcaccag ccagccatca tcaatcaata cccgatcggt ttcacgcaat
     1621 aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca gtagacaaac atcaacggat
     1681 ttatcggcaa aaggaagatg aagtggatcc gcctgcacct ggacgggcat tccttgcgca
     1741 gaaacattca cttgatgaga aaccgcgcac gcttcgcaat tgatttctgc gcttaaattg
     1801 ccaatcttaa gcagatgaaa accatacatt ttagtgaacc atgggttgag ctggcgctcc
     1861 agcgccttgc gataaagctc tccccagggc aaatcgcccc agcgatcagg agccacgaca
     1921 gtttgaggga ctcttgccgg tttcatcaca accttccgtt tcacactaag aggtaatcta
     1981 tgaatcttaa cagtattccc gcctttgatg acaattacat ctgggttttg aatgatgaag
     2041 caggtcgctg cctgattgtc gatcccggag acgcagagcc agtattaaac gccattgccg
     2101 ccaataactg gcaaccggag gccatatttc tcacccacca tcatcacgat cacgttggcg
     2161 gcgtaaaaga actggtggaa aagtttccgc aaattgtggt gtatggtcca caagagacac
     2221 aagataaggg aacaacacag gtagtcaaag atggcgaaac tgccttcgtt ttggggcatg
     2281 aatttagtgt aattgccaca ccgggtcaca ctttaggaca tatctgttac ttcagtaaac
     2341 cttatctatt ttgcggcgac acgctgtttt ctggtgggtg tggtaggttg tttgaaggga
     2401 cagcatcaca aatgtatcaa tcacttaata agttaagtgc gttacctgac gatacattgg
     2461 tatgttgtgc tcatgaatat acgttatcaa atatgaagtt tgctttgagt attcttccgc
     2521 acgatttgtc cataaatgat tattatcgta aagttaagga gttacgggca aaaaatcaaa
     2581 taacactacc cgtaattctg aaaaatgagc ggcaaattaa tgttttttta agaacggaag
     2641 atattgattt aattaatgta attaatgaag aaacattatt gcaacaacct gaagagcgtt
     2701 ttgcatggtt aaggtcaaag aaagataggt tctgataaaa ctttcttgtc atcggctccg
     2761 ttcgccgtta tgatcggtcg tcttttaagc aactattgac acacacatga aggcaaaagc
     2821 gatattactc gcctctgtcc tgctcgtggg ttgccagagt accggcaacg ttcaacagca
     2881 cgcacagagc ctttctgcag ctggtcaagg ggaagcagca aagtttacaa gtcaggcacg
     2941 atggatggac gatgggacgt ctatcgcgcc agatggtgac ttgtgggctt tcattggcga
     3001 cgagctaaag atgggaattc cggaaaatga ccggattcgc gaacagaaac agaaatattt
     3061 acgcaataag agctatctcc acgatgtaac tttacgggca gagccgtata tgtactggat
     3121 agcagggcaa gttaaaaaac gtaacatgcc tatggaactg gtactactac ccatagtgga
     3181 gagcgctttt gatcctcacg caacgtctgg cgccaatgcc gcaggcatct ggcagatcat
     3241 tccgagcacg gggcgcaatt atggtttgaa acagacccgc aattatgacg cgcgtcgcga
     3301 tgttgttgct tcaacaactg ccgcgctgaa catgatgcag cgtctgaaca agatgtttga
     3361 cggcgactgg cttctgaccg tagcggctta taacagcggc gaaggtcggg tcatgaaggc
     3421 aattaaaacg aacaaagcgc gtgggaaatc cacggacttc tggtcgttac cgttgccgca
     3481 ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt gatattctca aaaacagcaa
     3541 gcgttatggc gtacgtctgc caacgaccga tgaaagccgt gctctggcgc gtgtgcacct
     3601 gagcagcccg gttgaaatgg cgaaggttgc agatatggcg gggatttccg tcagcaagct
     3661 gaagacattc aacgctggcg taaaaggctc cacgctgggc gcaagtggcc cgcagtacgt
     3721 gatggtgcca aagaagcatg cagatcaact gcgtgattct ctcgcttcag gcgaaattgc
     3781 tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt aacagccgtg tttacaccgt
     3841 acgctctggc gacacgcttt caagcatcgc ttcacgtctc ggcgtaagca ccaaagattt
     3901 gcagcagtgg aacaaactgc gcggatctaa gctgaagcca ggccaaagtt tgacgattgg
     3961 tgcaggcagt agcgcacagc ggttggcaaa caacagcgat agcattacgt atcgtgtgcg
     4021 caaaggcgat tcgctttcaa gcattgctaa acgccacggc gtgaacatca aagatgtgat
     4081 gcgctggaac agtgacactg cgaatctgca accaggcgat aagctgacgt tgtttgtgaa
     4141 aaacaacaac atgccagatt cctgacaaac cagataataa aaaaggcacc gattcccccg
     4201 gtgccttttt tatttatgcc gctttatgcg cttctaccat gatgatatca ctggtgaaag
     4261 agccatcagt ctgcaaggaa aaatacgttc tcacctctgt cgatgcgctc tgttggtaaa
     4321 tgcgaatagc gtctactaac gcttctggcg tacgcattct cacgacccat gaagaaaatt
     4381 ccagcggtaa cttatctgta attaaattat caactatcag attggcttca ttgattaacg
     4441 tcaaccacgc accgctggcg tagtttcgta cgtgagaggt atcgcgtaat gcttctaccg
     4501 cctgtaacca gatgtcgcgc actgggtgac ccggagacat tacatccatc acaatcagcc
     4561 taccgccagg tttcaatatc ctattcactt ctcgcagtgc tgcaccaaca tcatgccagt
     4621 gatgggcaga ataacggctg ataacaatat caaatgcgtt atcggcaaat ggcagacttg
     4681 cggcatatcc ctggcgggtg gtgatatttt tcagttgccg ggcttcggca gcttgtgcca
     4741 caacatccag catgtgggcc gataagtcat acgccaccac cgcgctcacg ttttgcgcag
     4801 cgccaaacgt tggtctgccc tgtggcagac ctgacatacc ttatttatca ggcttgccgg
     4861 gactgaattc aacgagtagc ggattgtgat cggaagcgcg cgtaaccagt acagaagctt
     4921 cactgacgtt cagaccacgg tagaaaacaa aatcgagcgg gcgaccaaac gcccgacggc
     4981 gctgatcatc ggtaaaacgc acctggcgca gcgacatttc ccgcgcaaag cgatataacg
     5041 cgttcatcct tctacggctc caggcattga aatctcccgc cataatgacc gggccgctgt
     5101 ggtgagctat ctgatcacca ataggaagta actgcttact atagacatcc acgcccagac
     5161 tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg ggtgtcaggt aatggataga
     5221 ccgtcaccag tgccgacttc gccagacgca aaatgggttc tcgttcacgt aacgggcagc
     5281 aatacactgg atgtgccgcc gaaagggtca ttacgccaga aggatgttgt ggcagcacga
     5341 aagcgggtac ctgatcggcg gcaagatagt tagcggtcgc aaactgtact aactctggcg
     5401 ttgtctgcgc ttcctgcaat aacaccaggt gtgcatcttt gccgtagttc tttaataccg
     5461 acaaccattc agcgcgttgc tgtttgtata tgttccacac caggatccga atacgctctt
     5521 cggtacttaa cggttcccca ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg
     5581 gtaagatcct ttccgcaggt tgtccggcaa catagcgcat ggcataggtg ttttttcgca
     5641 cttttgactt tgaaacctct attacgtcaa cccttccgca ctcgggaggg ttctactatt
     5701 atagggattt tagctcactc tttcaacgag caattgtata ttgttatgta agcaagtgct
     5761 tacaccggtc agaattttgc tcattatgga gaacattaaa tcgcatgaca tatcacgcat
     5821 cctgatggtt taatgacaaa taaattcaaa atatctcttc tattttgcaa caggagcaaa
     5881 aatgaaagcc acgtcggaag aactcgccat ttttgtttcg gtcgtcgaaa gcggcagctt
     5941 tagccgggca gcggaacaat tagggcaagc aaactcagcg gtaagccggg cggtgaaaaa
     6001 gctggagatg aaacttggcg ttagcctgct taatcggacc acgcgacaac ttagcctgac
     6061 ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt ttgcaggaga tggcagcggc
     6121 agaatcagaa attatggaga cgcgtaatac accgcgtgga ctgttacgga tcgatgccgc
     6181 aactccagtg gtgctgcact ttctgatgcc gttaattaag cctttccgtg aacgctatcc
     6241 ggaagtcact ttgtcactag tctcctccga aacgattatt aatttgatcg aaagaaaagt
     6301 ggatgtcgcg atacgcgctg gtacgttaac ggattccagc ttacgtgcca ggccgttatt
     6361 taacagttat cgaaaaatta tcgcctcccc cgattatatt tcccgctacg ggaagccaga
     6421 aacgatcgac gatttaaagc aacatgtttg cctgggattc actgaacccg cttccctcaa
     6481 tacctggccg atagcctgta gtgatggaca attacatgag gtgaaatacg gtttgtcatc
     6541 caatagtggg gaaacactga aacagctttg cctgagtggc aacgggattg cgtgtttgtc
     6601 cgactacatg atcgacagag aaatcgctcg cggagaattg gtggagttaa tggcagataa
     6661 agtgttgcca gtggaaatgc cattcagtgc cgtctattac agcgaccgtg cggtaagtac
     6721 gcgcatccgg gcttttatcg atttccttag cgagcatgta aaaacagctc ccggaggagc
     6781 tgtcagagag gcttaatccc attcaggagc cagaccttcc gggctaacca ggcggtcgtt
     6841 gcaatccagt gcggcgatcg cttttttatc ttcggcatca agctgtaaat tttgtgcctt
     6901 aagattactt tccaggtttt tacgtttagt agaagaagga attactgagt aaccttcccc
     6961 catagcccac gccagaatca cttgtgccgg agtcgcattg tgtttagctg cgatacgagc
     7021 aataacctca tctttcaggg ccttaccata cgccagcgtc atataggaag taatatgtat
     7081 gccgtgctgt ttagcccagg caaccacttt acggttttgc agataaggag agagttcaat
     7141 ctggttagta gcgatgtttt cagcgccaac agcagcaatc gccttttcca tcaatgggat
     7201 cgtgaagttg gaaataccga tctcacgcgt cagcccttgt tttttggctt ccagcagcgc
     7261 ctgcataacc ccttcaacag agacttcatc gtttggtgac ggccagtgga ttagcgtcag
     7321 atcaacataa tcggtacgca atttttgcag gctctctttc agacttggga tcaatttgtc
     7381 tttgctgaga ttttcaatcc agattttagt ggtgatgtag agttcatgac gtggcacgcc
     7441 actttctgca atcgcctgac ctactgcggc ttcgttatca tagatttgtg cggtatcaat
     7501 tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa ataacaacgt agtctttcaa
     7561 cctaaaccaa atgcagggat agccataata tgcctctttt aatgctattt tcgttaactg
     7621 aggtgattat gggcttcaga ctgttgagga aaaaagcgta aaatgcagag gatttttgcg
     7681 attctggcaa taatagatat acagacgaaa aaaaacctcg agcgttaact cgaggcttct
     7741 taata
//
LOCUS       DAJTNN010000181         7629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTNN010000181 DAJTNN010000000
VERSION     DAJTNN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.183"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            554..3967
                     /locus_tag="OO150_003533"
     CDS             554..3967
                     /locus_tag="OO150_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5517547.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRN
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQIARLQKIVEELGVVTDAATV"
     gene            4031..5665
                     /locus_tag="OO150_003534"
     CDS             4031..5665
                     /locus_tag="OO150_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5517548.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5674..6744
                     /locus_tag="OO150_003535"
     CDS             5674..6744
                     /locus_tag="OO150_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5517549.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLIGF"
     gene            complement(6869..7585)
                     /locus_tag="OO150_003536"
     CDS             complement(6869..7585)
                     /locus_tag="OO150_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517550.1"
                     /translation="MLSETKKRIPAHLFRMIKDELYELVLTDDPKGKLYVRDSEKIEE
                     GVSTTEFVVGYGAISMVKKSESMATKGLVGLERVDLIREVVFENGYYDWGCVVNDVLP
                     NICKGNARIPIFHFLNHAKLINPDGSIINETELSGGVLSRLNITPISFQSQGWDKRRS
                     ETVPEVKVGVNELYLAYDFSFFLRMMPYMEPGLIKRDMGELLKILKKHIDEAMNTQVL
                     SSNFCKLVCIYDYIKNSHRY"
BASE COUNT         2221 a         1735 c         1790 g         1883 t
ORIGIN      
        1 ccaaatatca tggtattgat acgggagatt atgccgtaca ggtacgacac gttatgtcgt
       61 gccaaagggg agtggataga aaaaatggcg aagatgcgaa aagttgatta atgcattgaa
      121 aaaaatgaat taataatgaa cttgggtaaa agcgccatca aaatacgaca cgacctgtcg
      181 tattttgtgt tagccgggaa aagatatctg ctacttacta aaatgattta gtgctgaaaa
      241 tatttataag ccaataaata gacaggtatt acggagtaac gcccaggcta ttttaacgag
      301 cctgctgttt tattggctca aaaaacatat cccatagatg atcagccata cccgcgttag
      361 tgagcaaggt atcccttgag tagccagggc tgaagacgcc cattgatggg cgaaaaccaa
      421 ggttctggcc gttaaagcgg ctatgtcgtt aaacacatca ccaccatatg ccatccggct
      481 cacttcgtcg gccaaattag tgtgatattc tttccagaat ttctgttgta tccacaacat
      541 aaggcgatga ttgatggagc agtctctcaa ttttgaaatg ttgcgcagcc agtggccgga
      601 actggcagaa ctcgcatgta tggcagagcg ctatgttcac tccgatccgg aaagctgcct
      661 ggttaagctg cgcaactaca ccgaattgat ggtgcgctgg ttgtatcgtc aggagcggtt
      721 gccggaaggt attaaggcta atctttacga tttaatgaac gctgatgtct ttaccagcat
      781 gatgccggaa gccatcatca tgaaaatgga tgcgctgcgt atccatggca accgtgccgc
      841 gcacggcgga cgtatcaaag ctaaagatac ttactggctg ctcaaagaag cgtatttgtt
      901 gggaatttgg ctgtatgttc gctacgccca cggtaatgtt gatgactgcc ccaaatttac
      961 actccctcca ttaacacaat cttcaggtcg tgcagatgaa aaacgtctgg aagatgcaat
     1021 caaggctcag gatgaaagcc gtgagcgcga actggcgcta caacgcgcac tacagcaaga
     1081 acaggaaaag gccgaacacc tcactcaacg cctgaatgaa gccagagcgc gtaaccagca
     1141 tgttgccgat attctctcta ttgatgaagc ggaaacccgc cgtcgcctga ttgactctcg
     1201 cttacttgca gccgactgga atgtagggga agaacttaaa aacacggatc aagtcacaca
     1261 agaacatcca gtcaaagagc aacccaccac ctctggtgac ggttatgcag actatgtctt
     1321 gtgggatgag gcacacaaac cgttggcggt ggtggaagca aaaaaaacca gcgtcaatgc
     1381 cgagcaagga cgaattcagg cccggctgta tgcagactgg ctggaaaaag aatacggtca
     1441 gcgtccggtc atcttctaca ccaacggcta tgatatctgg ctgtgggatg accataaaac
     1501 tcatggttat cccccgcgtc gggtgtttgg cttctacagc aaggaaagcc tgcaatatct
     1561 tattcagcag cgtgaaaccc gtctgccgct gaacagagtg ccacacgtaa aagataacga
     1621 aggtaaggcc gttgccggac gtttgtacca gctcgaaacc attgcccgcg tcagtgaacg
     1681 gtttaccaac aagtaccgac aatcgttaat tgttcaggct acgggtacag gcaaaacccg
     1741 cgtggcaatt gcactaagca aactgatgat tgatgcccgc tgggtaaaac gcgtcctgtt
     1801 tctttgcgac cgtaaagaac tgcgtaaaca ggcggcaaat gcctttaatc aattcaccaa
     1861 tgagccgctg tatgtggtcg ggaaatcgaa aaaagcagac aggcagaatg ccagaatcta
     1921 catcgctacc tatcccggta tgatgaaaat catggaccat ttcgatgtcg gttatttcga
     1981 tttgatcatc gccgatgaat cccaccgctc tatctacaac gtctacggcg atctgtttaa
     2041 gtattttgat gccctccaga ttggcctgac ggccacgcca attgacatgg tgagtaaaac
     2101 cactttcggc ctgtttggtt gtgaaggacg tattcctacc gccaactaca gcctggaaga
     2161 tgccatcgcc gataacaatc tggttcccta tgaggttgtc acacacacaa cagaattcct
     2221 gcgcgaaggt atcaaacggg agaaattaag cgacgcacaa atccgcgagc tggaagaaca
     2281 aggcatagat cccaatacgc tggagtttga tggcaaggcg ctggatgagg cgatctacaa
     2341 caaagacacc aaccgctata tcctgcgtaa cctgatggaa aatggcctga aagatcggga
     2401 cggccagttg cctggtaaaa ccatcatttt cgcccgtaac cacaaacacg cgctgctgtt
     2461 gaatgaattg ttcgacgata tgtacccaca gtttgccggg cgcttctgcc aggtcatcga
     2521 caactacgat ccccgcgccg agcaactgat tgatgatttt aaagggctgg atgaaagcac
     2581 caacaaagag ctgaccatcg ctatctccgt tgacatgctc gataccggta ttgatgtccc
     2641 ggaaattgtg aatctggtct ttgccaaacc ggtcaaatca aaagtgaagt tctggcagat
     2701 gattggccgt ggtacgcgcc tctgtccggg gttgtacggt tacgacgata acggcaaacc
     2761 gctggataaa cagaaattcc gcattttcga tcactggggc aattttgagt atcacgagct
     2821 gcataccgaa gaggcggaag tcacagcgac aaaatcgctt gcgcaaaaac gcttcgaagc
     2881 gtgggttatg ctgggggccg ctgcgcagcg taaattcgac aaacaggcgg tggatttagt
     2941 tgctcaccag cttcgcgagc aaatcaacgc actggatgaa aagtcgattg ccgtacagga
     3001 aaaatggcag cagaaagcgc agtacagtga tgaaaaggtg ctgcgccagc tttctccgaa
     3061 aacacagcag gatctgctat ctgttctggc cccgttgatg cagtggctgg acgtgcgcgg
     3121 gcaaagcgat gccatacgct ttgatatgga tattctggcg gcgcaaactg cccgttatac
     3181 caacccggaa gagctggatg ttctctggcc gatcatcgtc gaaaaagtgg agcgcctgcc
     3241 gccgcatttg gcgcaggtgc agcaacaggg acaacgaatc aatcagcttc gtaatttaag
     3301 ctggtggaag caggccagcc tggaagagct ggaggatatt cgcattcatt tgcgcggtat
     3361 catgcacctg atggaaaaag acgcgacgcc taaatttggt tcgatacagg tggatattac
     3421 cgaagatgcg aacctgatcc agacggagac ccgcaaaact aatatccgct cgattgattt
     3481 caaactctat cgccagcagg ttcagggggc gctggagccg ctgttccagc aaaacccggt
     3541 tctgaagaaa atccgcaacg gcgagccagt cacgcaaaac gagctggatg agctggcgaa
     3601 gctggtgctg atccagaacc ccaacgttga tattcgtgcg ttgaaagagt tttatccgca
     3661 ggcaaccgcc agcctggata aacttttgcg taccatcatc gggatggaca gcgacgcggt
     3721 ggaagtgcgt tttgcccagt tcgccgctga taacagcctg accagccaac aactgcgctt
     3781 cctgtcattg ctgaaaaacc acattcgcga ttacggcacc attgaaatgc ggcagctctt
     3841 tgaacagcct tttacgcata tccacaacga aggcgttacc ggtgtgttcc ctgatatagc
     3901 gcaaatcgcc cgcctgcaaa agatagtcga agagctgggt gttgtgaccg acgcagcgac
     3961 ggtataatac acgcatgaca agccccggca gcggggcttt tgtatttaaa acaggatata
     4021 aacgctaaca atgattactg gcgacttaaa aagtaaaatc gacggactat gggaagattt
     4081 ctgggtgggt ggtatcacca acccgctgac cgtaatcgaa cagatcacct atctgatgta
     4141 ttcccggatg ctggataccc aggaacaacg cgacgaaaag cgtaaacaaa tcgcgggtat
     4201 tgattttaaa ccgcgttttg cgccagaaca gcaggagttc cgtttcagtc actatagcaa
     4261 ccttggctcg gatgagatga tggaagtggt gcgcgatggc gtattccagc atttccgtca
     4321 gctcggccag gccgatgctt cgaaggtgac gctgctgggc aactttatga aagatgctcg
     4381 tctggagatt gttaagccgt cattgctgac caaagcggtt gaggtaatca aaaacctgcc
     4441 attggatcgc ggcgacacca aaggcgacct ttacgaatac ctgttaagca agctgacaac
     4501 tgccggaatc aacggacagt tccgcacacc gcgccacatt atccgcacga tggttgaaat
     4561 gatggagccg aacccggccc gcggcgagac gatttgcgat cccgcctgtg gcaccggtgg
     4621 ttttctggca accagctatg aatatctgct ggagaagtac agctcgctgg aatccattca
     4681 tactgagatt ggcaccaacg aacgtggcga gctggaagag caaaaaatct ttaccggcga
     4741 tctgctgaca ccgtggcgta accatgtgga taacaacatg ttccacggtt acgactttga
     4801 caccacaatg ctacgtatcg ccgccatgaa cctgattatg cacggcgtgg atgcgcctga
     4861 tatccactat caggacacaa tgagccagag tttcagcaca aacttcccgc aggccagtaa
     4921 aaacgccttc aacctgattc tggcgaaccc gccgtttacc ggttcactgg acgaggaaga
     4981 tatcgactcc acgctgtcgg caatggtgaa aaccaaaaaa accgaactac tgttcctggc
     5041 gcgtattctg caaatgctga aagtgggcgg gcgcagtgcc actatcgtgc cgcagggcgt
     5101 gctgtttggc tctagcaagg cgcaccagtc acttcgcaaa acgctggtgg aagataacca
     5161 actggaaacg gtgatcaatc tgccttctgg tgtatttaaa ccttacgctg gcgtggcgac
     5221 ggcgatcctg atctttacca aaggcggtca aacggatgag gtctggttct acgatctaca
     5281 aaatgacggc tacagcctgg atgataagcg caacccgata aaagacaacg atctgccgca
     5341 tctgctggca agctggaagc attaccgtac tttacgcggg ctaccggttg ataactttat
     5401 gggtaagaag ttagcctcgt tgcttaaaca gcagtacccg gaagggatta atgctggcgt
     5461 tgattttaaa gatcgcacgc aggcggcgtt tgttgtaccg aaagcggata ttgctgcgca
     5521 gaaatacgat ctatccatca accgttataa agaagtcgtg tatcaggcgg aggaatatga
     5581 agatccgaag gtgatattga agcggttaaa ggatctggaa aaagagattc tggcggattt
     5641 ggatgagctg gaggggatgc tgtgagttcg gcgatggtga agttagggga tgttattaat
     5701 gttcactacg gaaaagcatt aaaagctgat cagcgtgtat cgaatggaag tgtccatgtg
     5761 tttggtagtt caggaattgt cggcaaccac gataaaacgc tttgcagcta tcccacgata
     5821 attattggcc gcaaaggcag cgttggtgct attacctggg ctccaagcgg aggctggatc
     5881 attgatactg cttattacgt tgagatcaaa gataataata agttagatct ccgatacttg
     5941 ttttatattc tgagcggaat tgatctcact aagaaaacga ttaccacaag tattcctgga
     6001 ttaaatagag acgatctcta tgatacattt attaaattac caccttttga agagcagaaa
     6061 cgaattgtgg atcttctcga taaagctgag ggtatacgcc aaaaacgtga gcaatccatc
     6121 aaactggctg atgatttttt gcgtgctacg tttgctacaa tgtatgggaa tcctattaca
     6181 aaccccaaaa aatggcctgt ccatcttatg ggagaaatta ttgaatttaa aggtggaaat
     6241 caaccaccta aaagtgattt tatttttgaa cctaaacagg gttatattag attagtacaa
     6301 attagagatt ttaaatccga taaatatgcg acgtacattc cgcaagaaaa agcaaaacgt
     6361 attttcgaag ttgatgatgt tatgattgct cgttatgggc cacctgtgtt tcaaatactt
     6421 agaggtttat ccggcagcta caatgtagct ttaatgaaag cttcacccaa agaaaatatt
     6481 aggaaggggt ttatattcta tcttctacaa cttccggaat atcatgatgt tgttgtaaaa
     6541 aactctgaaa gaacagcagg acaaaccggt gtaaatcttg aactattaaa caaattcaat
     6601 gttcccctcc cacctatata ctatcaagat gagatattag atagattagc tagaattgaa
     6661 aaatttaaag aaaaaataga aatatcatta aatcatttag aaatacaatt tttatcacta
     6721 cagaagagat taataggttt ttaaatcctt agtatcgaaa taaacattaa tttattatat
     6781 taaattaaga tattttagga aattaaggcg tcactcttag gtaacgcctt tttttactat
     6841 ctcgccccct aagaaggtta tatcttactt aatacctgtg actatttttg atataatcat
     6901 aaatacacac taatttgcaa aaattacttg acaatacctg agtattcata gcttcatcta
     6961 tatgtttttt caatattttg agaagctctc ccatatcgcg cttgattaat ccaggctcca
     7021 tataaggcat cattcttaaa aagaaactga aatcataagc aagatataac tcattgaccc
     7081 ccaccttgac ctcaggaacg gtttcagagc gtcgtttatc ccatccttga gactgaaaac
     7141 taataggagt gatatttaat cgtgataaaa cgccacctga gagttctgtt tcattaatta
     7201 tactcccatc aggattaatt aatttagcgt gatttaagaa gtgaaaaata ggaatgcgcg
     7261 cattgccttt gcatatattt ggcaaaacat cattaacaac acatccccaa tcataatatc
     7321 cattttcaaa tacaacctct cggatgagat ctacacgctc cagtccaacc aatcctttag
     7381 ttgccatcga ttccgatttt tttaccatcg aaatagcacc atatcccaca acaaactcag
     7441 ttgtactgac cccttcttca attttctctg aatccctcac atataatttt cctttcggat
     7501 catcagttaa gaccaactca taaagttcat cttttatcat tcggaataaa tgtgctggaa
     7561 tccttttttt agtttcactc agcacctcaa atatttcttt ataggatggt gcctgaacat
     7621 attttaccg
//
LOCUS       DAJTNN010000182         7621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTNN010000182 DAJTNN010000000
VERSION     DAJTNN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.184"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..453
                     /locus_tag="OO150_003537"
     CDS             <1..453
                     /locus_tag="OO150_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HCR5517551.1"
                     /translation="AIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETV
                     YINENNESYLKAQFEKLSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDP
                     DILSKMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            816..1853
                     /gene="dusA"
                     /locus_tag="OO150_003538"
     CDS             816..1853
                     /gene="dusA"
                     /locus_tag="OO150_003538"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5517552.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1987..2229
                     /gene="pspG"
                     /locus_tag="OO150_003539"
     CDS             1987..2229
                     /gene="pspG"
                     /locus_tag="OO150_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5517553.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2394..3377)
                     /locus_tag="OO150_003540"
     CDS             complement(2394..3377)
                     /locus_tag="OO150_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5517554.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3460..4875
                     /gene="dnaB"
                     /locus_tag="OO150_003541"
     CDS             3460..4875
                     /gene="dnaB"
                     /locus_tag="OO150_003541"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5517555.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4928..6007
                     /gene="alr"
                     /locus_tag="OO150_003542"
     CDS             4928..6007
                     /gene="alr"
                     /locus_tag="OO150_003542"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5517556.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6260..7453
                     /gene="tyrB"
                     /locus_tag="OO150_003543"
     CDS             6260..7453
                     /gene="tyrB"
                     /locus_tag="OO150_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5517557.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1836 a         1830 c         2001 g         1954 t
ORIGIN      
        1 gcaataggtt ctggtttttt aacgtttgtt aatgcctgcc aggaacatga tgacaacagc
       61 ttaaaattat ttgcttcgct gttaatttca ctttcatatt ctagtgccta cgcagattta
      121 tcagaaacag tgtatattaa tgaaaataat gagagctacc tgaaagctca gtttgaaaaa
      181 ttatctcaac gtgatatgaa gaagtacctg ggagagatga agcgtctggc tgatggggga
      241 gaaatgaatt ttgatggcta tctggataag atgtcacatc tggtgaatga agggacgctc
      301 gatcctgata ttttaagcaa aatgcgagat gctgcaccac aattaattag cttcgcgaag
      361 tcgtttgacc caacctcaaa ggaagagatt aaaatactta cagacacttc taaattaatt
      421 tatgatttgt tcggggttaa atcggagaaa taatatgtga agttcttcga tagtatggaa
      481 ggcattatat aaaaggaccc aatatttatt gggtcctttt ttctctatca atgctattag
      541 cagggagata tatcaccaaa gtttaatgtg tgatttttta tttatcgtcg aacctggatt
      601 gtttatcatt ggccttaaca aagttaacgg ctaataagat tatttccatc acttcgtgag
      661 agcttcatgc cttgagagga tctcaatttt cttttgcaat gagacaggcg cttcctgttg
      721 ttatggtata gtaccccgct attgagcctc ctgaatagtg atgctgaata acataacccc
      781 atgatatatc gataaaataa tctctacatt tgaaaatgca cggtaattct gaaatgcaaa
      841 aaatcaacca aaccagcgca atgcctgaaa aaactgacgt tcactggagt ggtcggttta
      901 gcgttgcacc aatgctcgac tggacggaca gacattgccg ctatttcttg cgtctgcttt
      961 cccgcaatac gttgctgtat accgaaatgg tgaccacagg ggcgattatt cacggtaaag
     1021 gtgattacct ggcgtacagt gaagaagaac atccggtagc gttgcaactc ggcggtagcg
     1081 atccggcggc gctggcacag tgtgcgaagc tggcagaagc gcgtggatat gatgagatca
     1141 acctgaatgt cggctgcccg tctgaccggg tgcagaacgg catgtttggt gcgtgtctga
     1201 tgggtaatgc gcagctggtt gccgattgcg tgaaagcgat gcgcgatgtg gtgtcgattc
     1261 cggtgacggt gaaaacgcgt attggcatcg atgaccagga cagctatgaa tttctctgcg
     1321 atttcatcaa caccgtttcc ggcaaaggcg agtgtgggat gttcatcatc catgcacgta
     1381 aagcctggct ttcggggtta agcccgaaag aaaaccgtga aatcccgccg ctcgattatc
     1441 cgcgtgtgta tcaactgaag cgtgactttc cgcatctgac aatgtcgatt aacggtggta
     1501 tcaagtcgct ggaagaggcc aaagcacacc tgcaacatat ggatggcgtg atggtcgggc
     1561 gcgaggcgta tcagaatccg ggtattctgg cggcggtaga ccgggagatc tttggttcct
     1621 cggataccga tgcctatccg gtggcggtag tgcgcgccat gtatccgtac attgagcgtg
     1681 aactcagcca ggggacgtat ctcggccata ttacccggca tatgttgggc ttgttccagg
     1741 gtattcctgg cgcgcggcag tggcggcgtt atttaagtga aaatgcccat aaagcgggtg
     1801 cagacattaa tgtgctggaa cacgcgctca aactggtggc ggataagcgt taacttttca
     1861 ccaaaaagta gtcaaattca ccacgccctg cgcaccgtcg cggggcgttt tgctgttaaa
     1921 tcaatagatt atttttggca tgattcttgt aatgccagca agagatttca tatttgggag
     1981 agcatcatgc tggaactact ttttgtgatt ggcttttttg tcatgctgat ggtcaccggc
     2041 gtttcgttgc tgggcattat cgccgcgctg gttgtggcga cggccattat gttcctcggc
     2101 ggtatgctgg cattgatgat taagttgctg ccgtggttac tactggcgat tgcggtggtg
     2161 tgggttatta aggcgattaa agcaccaaaa gtgcctaaat atcagcgtta tgaccgctgg
     2221 cgttactaag ggattgtgcg gatgatcaca acctaaggtt ttatccttag aacaaaatag
     2281 gaattgataa tcaaatctgt cactattgcg cctctaacag attcatcgtg ctgtacccta
     2341 catacagccg actataaaaa gaaagggctt cccaggtgga agccctattt cttttatgga
     2401 atcagcaggc tggaaccttg cgtcgcccgg ctttccagaa tctcatgcgc acgctgcgca
     2461 tccttcagcg gatatttctg ctgctcggcg acatcgacct taatcacacc gctggcaatc
     2521 aaagagaaca gttcattact ggcctcggtt aattcctccc gtgtggtgat atagccttgc
     2581 agggaagggc gtgtcacata caacgagcct ttttgattga gaatgcctaa gttcacaccg
     2641 gtaaccgcac ctgatgagtt gccaaaactg accattaagc cgcggcgttg caggcaatcc
     2701 agcgaccgtt cccaggtgtc tctgcccacg gaatcgtaca ctacgcgcac tttcttaccg
     2761 ccggtgatct ctttcaatcg ctcgaccaga ttctcttcac gatagttaat aacctgccac
     2821 gcgccggctt ttagcgcgct ctgcgctttt tgcgcggttc ctacggtgcc gataagtttc
     2881 gcgcccagag cttttgccca ctggcaggca atcaagccaa cgccgccagc cgctgcgtgg
     2941 aacaggaact gttcatcggg tttaatttca taggttttgc gcagcagata ataaaccgtt
     3001 aagcctttca ggaaagatgc tgcagcttgc tcaaaagaaa tagccgcagg cagaatcgcc
     3061 gctttatccg cattaatgtt atgcacagag ctgtaagcgc ctaacgccga ctgcgcatag
     3121 actacacgat cgcctgcctt aatatgcttt acaccactgc cgactttact cacgatgcct
     3181 gccgcttcgg tacctgatcc gctgggtagt gatggcggcg ggtaaaggcc gctgcggata
     3241 tacgtgtcga taaaattgat gccgatggct ttattttcga cctggatttc attctctgcc
     3301 ggatcggcag gagtgaactc tacggcttga agtacttccg ggccaccgtg cttgtgaaat
     3361 tcaattcgtg ttgccatgtg tcctccagaa cgtatcgtca gggtctgctt catatggtaa
     3421 agtttcgacc cattctttat ctcggtaact ccattcacta tggcaggaaa taaacccttc
     3481 aacaaacagc aggctgaacc ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct
     3541 ccgcactcga tcgaagcgga gcagtcggtg ttgggcggtt taatgctgga taacgaacgc
     3601 tgggacgatg tagccgagcg tgtggtggca gacgattttt acacccgccc acaccgccat
     3661 atctttactg aaatggcgcg tttgcaggaa agcggtagtc ctatcgatct gattaccctt
     3721 gcggaatcgc tggaacgcca ggggcaactc gatagcgtcg gtggttttgc ttatctggca
     3781 gagctgtcaa aaaatacgcc aagtgcggcg aacatcagtg cttatgctga catcgtgcgt
     3841 gaacgtgccg ttgtccgtga gatgatctcg gttgcgaatg agattgctga agccggtttt
     3901 gatccgcagg ggcgtaccag cgaagatctg ctggaccttg ctgaatcccg cgtctttaaa
     3961 attgccgaaa gtcgtgcaaa caaagacgaa gggccgaaga acatcgccga tgtgctcgac
     4021 gcaaccgtgg cgcgtattga gcagttgttt cagcagccac acgatggcgt taccggagta
     4081 aacaccggtt atgacgatct caacaaaaaa accgctggct tgcagccgtc ggatttgatc
     4141 atcgtcgccg cgcgtccgtc gatgggtaaa acaacatttg cgatgaacct cgtcgaaaac
     4201 gcggcgatgt tgcaggataa accagtactt atcttctcgc tggagatgcc ttcagaacag
     4261 attatgatgc gttctctggc gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg
     4321 cagctcgatg atgaagactg ggcgcgcatt tccggcacca tgggtatttt gctcgaaaaa
     4381 cgcaatatct atatcgatga ctcctccggc ctgacgccaa cggaagtgcg ttcccgcgca
     4441 cgacgtattg cccgtgaaca cggcggcatc gggcttatca tgatcgacta cctgcaactg
     4501 atgcgcgtac cggcgctttc cgataaccgt acgctggaaa ttgcagaaat ctctcgctcg
     4561 ctgaaagcac tggcgaaaga actgaacgtg ccggtggtgg cgctgtccca gttgaaccgt
     4621 tctctggaac aacgtgccga caaacgcccg gtcaactccg acctgcgtga atctggctct
     4681 atcgagcagg atgcggactt gatcatgttt atctatcgtg atgaggtgta tcacgaaaac
     4741 agtgatttaa aaggcatcgc ggaaattatt atcggtaaac aacgtaacgg cccaatcggg
     4801 acggtacgcc tgacctttaa cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag
     4861 tacgacgacg aataataatt attttatgaa ttaggcaatt aaagcaaaca cttatcaagg
     4921 aacacaaatg caagcggcaa ctgttgtgat taaccgccgc gctctgcgac acaacctgca
     4981 acgtcttcgt gaactggccc ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta
     5041 tggtcacggt cttcttgaga ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc
     5101 ccgtctcgaa gaagctctgc gactgcgtgc ggggggaatc accaaacctg tactgttact
     5161 cgaaggcttt tttgatgcca gagatctgcc gacgatttct gcgcaacatt ttcataccgc
     5221 cgtgcataac gaagaacagc tggctgcgct ggaagaggct agcctggacg agccggttac
     5281 cgtctggatg aaactcgata ccggtatgca ccgtctgggc gtaaggccgg aacaggctgg
     5341 ggcgttttat catcgcctga cacagtgcaa aaacgttcgt cagccggtga atatcgtcag
     5401 ccattttgcg cgcgcggatg aaccaaaatg tggcgcaacc gagaaacaac tcgctatctt
     5461 taataccttt tgcgaaggca aacctggtca acgttccatt gccgcgtcgg gtggcattct
     5521 gctgtggcca caatcgcatt ttgactgggt gcgcccgggc atcattcttt atggcgtctc
     5581 gccgctggaa gatcgctcca ccggtgccga ttttggctgt cagccagtga tgtcactaac
     5641 ctccagcctg attgccgtgc gtgagcataa agccggagag cctgttggtt atggtggaac
     5701 ctgggtaagc gaacgtgata cccgtcttgg cgtagtcgcg atgggctatg gcgatggtta
     5761 tccgcgcgcc gctccatccg gtacgccagt gctggtgaac ggtcgcgaag taccgattgt
     5821 cgggcgcgtg gcgatggata tgatctgcgt agacttaggt ccacaggcgc aggacaaagc
     5881 cggggatccg gtcattttat ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac
     5941 aaaagtgagc gcttacgaac ttattacgcg cctgacttca agggtcgcga tgaaatacgt
     6001 ggattaatcg ttctgtaata tttgattgtc tgtgccggat gcggcgtgaa tgccttatcc
     6061 ggccaataaa atcctaaaaa ttcaataagt tgatgttctt tcatgctctt ataaaggtcg
     6121 tgcctctggc ggatgttcgt ttgtcatgag tctcactctg ttgctaattg ccgttcgctc
     6181 ctgaacatcc actcgatctt cgccttcttc cggtttattg tgttttaacc acctgcccgt
     6241 aaacctggag aaccatcgcg tgtttcaaaa agttgacgcc tacgctggcg acccgattct
     6301 tacgcttatg gagcgtttta aagaagaccc tcgcagcgac aaagtgaatt taagtatcgg
     6361 tctgtactac aacgaagacg gaattattcc acaactgaaa gccgtggcgg aggcggaagc
     6421 gcgcctgaat gcgcagcccc atggcgcttc gctttattta ccgatggaag ggcttaacag
     6481 ctatcgccat gccattgcgc cgctgctgtt tggtgcggac catccggtac tgcaacaaca
     6541 gcgcgtagca accattcaaa cccttggcgg ctcaggggca ttgaaagtgg gcgcggattt
     6601 cctgaaacgc tacttcccgg aatcaggcgt ctgggtcagc gatcctacct gggaaaacca
     6661 cgtagcaata ttcgccgggg ctggattcga agtgagtact tacccctggt atgacgaagc
     6721 gactaacagc gtgcgcttta atgacctgtt ggcgacgctg aaaacattac ctgcccgcag
     6781 tattgtgttg ctgcatccat gttgccacaa tccaacaggt gccgatctca ctaatgatca
     6841 gtgggatgcg gtgattgaaa ttctcaaagc ccgcgagctt atcccattcc tcgatattgc
     6901 ctatcaagga tttagtgccg gtatggaaga ggatgcctac gctattcgcg ccattgccag
     6961 cgctggatta cccgctctgg tgagcaattc gttctcgaaa attttctccc tttacggcga
     7021 gcgcgtcggc ggactttctg ttctgtgtga agatgccgaa gccgctggcc gcgtactggg
     7081 gcaattgaaa gcaacagttc gccgcaacta ctccagcccg ccgaatttgg gtgcgcaggt
     7141 ggtggctgca gtgctgaatg acgaggcatt gaaagccagc tggctggcgg aagtagaaga
     7201 gatgcgtact cgcattctgg caatgcgtca ggaattggtg aaggtattaa gcacagagat
     7261 gccagaacgc aatttcgatt atctgcttaa tcagcgcggc atgttcagtt ataccggttt
     7321 aagtgccgct caggttgacc gactacgtga agaatttggt gtctatctca tcgccagcgg
     7381 tcgcatgtgt gtcgccgggt taaatacggc aaatgtacaa cgtgtggcaa aggcgtttgc
     7441 tgcggtgatg taatgcagga aagcaggctg gagttaccca gcctgcagtg aaattaaact
     7501 gtcgtcgctt tcactatttc tttgtagatg atttttttga tgccgttgtt ctacgtgaga
     7561 gataataaaa cgttgtcagt tcttttattg ttaagtttat cccaattaac tggaattcct
     7621 t
//
LOCUS       DAJTNN010000183         7572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTNN010000183 DAJTNN010000000
VERSION     DAJTNN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.185"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..194
                     /locus_tag="OO150_003544"
     CDS             <1..194
                     /locus_tag="OO150_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5517558.1"
                     /translation="RGAFFISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGT
                     NTLLACCCLLRPEEVNSEY"
     gene            184..357
                     /locus_tag="OO150_003545"
     CDS             184..357
                     /locus_tag="OO150_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5517559.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(406..1478)
                     /locus_tag="OO150_003546"
                     /pseudo
     CDS             complement(406..1478)
                     /locus_tag="OO150_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1550..4294)
                     /gene="torS"
                     /locus_tag="OO150_003547"
     CDS             complement(1550..4294)
                     /gene="torS"
                     /locus_tag="OO150_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR5517560.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4377..5366
                     /gene="torT"
                     /locus_tag="OO150_003548"
     CDS             4377..5366
                     /gene="torT"
                     /locus_tag="OO150_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5517561.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMIWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(5339..6031)
                     /gene="torR"
                     /locus_tag="OO150_003549"
     CDS             complement(5339..6031)
                     /gene="torR"
                     /locus_tag="OO150_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5517562.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQT
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGECYFLAADVC"
     gene            6151..7323
                     /gene="torC"
                     /locus_tag="OO150_003550"
     CDS             6151..7323
                     /gene="torC"
                     /locus_tag="OO150_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5517563.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            7323..>7572
                     /locus_tag="OO150_003551"
     CDS             7323..>7572
                     /locus_tag="OO150_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase"
                     /protein_id="HCR5517564.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRSATAAQAAD
                     VISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSK"
BASE COUNT         1847 a         1954 c         1945 g         1826 t
ORIGIN      
        1 ctcgtggagc cttttttata tctgccttat ttttcttcaa cgctgtatgt atagtaagcg
       61 ataacctgtt gattattgaa tctttcgggg agatggctta taacatttct tacctgacca
      121 gggtaccggg aaccaacacc ttactggcgt gttgctgtct tttaagacca gaagaggtta
      181 acagtgaata ttgaagagtt aaaaaaacaa gccgaaacgg aaatcgccga ctttatcgcg
      241 caaaaaatcg ccgagctgaa caagaataca gggaaagaag tctctgaaat tcgcttcacc
      301 gcacgagaaa aaatgaccgg gcttgaaagt tatgatgtca aaatcaaaat aatgtgattt
      361 tgtgaacatc accccgtgcg aggtgatgtt ccgcttgttg ctaatttagt gatcaatcat
      421 tggcgcttgt ggaattaagc gtcggtacaa ttcctccggc accgggcttt gccatactcc
      481 cgcatacatt gcgtaaccaa tcaccgcgaa cataatcccc agaaccagta gcgtcattaa
      541 ccagccagac aacgcaaaag cttttttatt tgccgcaggt ttttgcagtg aaaaggtcaa
      601 tgttgaggct accggacatg actctacgca agtcatacag ccggtacatt ccactgttcg
      661 tacctgaatt aatttatcta ccgggatccg tgatgggcaa ttttttgcgc atttgccaca
      721 gccgatacaa ctttcggcat tgcgacgaat cttaaacggc gataatagcg aaaccacgcc
      781 catcagcgcg ccatatgggc aaagataacg acaccaggca tggcgaataa acaggctggc
      841 aatcagcaaa acggtcacgc tgattaatgt cgcggtcccc atatgacgaa agaaatcgag
      901 catttttacg tccatcacca cgctgtaggg cgacaacata aaatagtgaa tcgcctgagc
      961 gggcatcaat aacgcgatat agagaaaaaa actcaacagc aaatacttca cgccgcgcag
     1021 aggaatatcc agccagcggg gaaggacaca ttgccgacca aacagtttgt taccgagatc
     1081 gccgattaat tcagaaagcg taccaaccgg gcataaccat gagcaaaagg cctttttgag
     1141 taatagactg atgacgataa aagcgaccaa taacagcatc gcggagcgtg gacggacggt
     1201 aactgacctg ttgtaaggct atatttcaga ttcatcagac cggcaatcgg tagccagcct
     1261 tcgatacctc ccggtctggc gacaaatgtc gtgctacttg ccgtttcgta atagcgcacc
     1321 caataccaga acgtgatggc aatataaata ttcattgcca acagtaataa ttgcgtcgct
     1381 ttacgccagg tcgtggcatt gcgccagtca ttccacggta atttgccgcc cgtcgtgcct
     1441 ggccgccgct gccagcgggt tctttttttc tctgccatga ttttgccagt ccgttaagtt
     1501 gcataccaaa tgccactatt ttagttgttc ttaactggct gatattgatt caaatcgcgt
     1561 tcaggtcttt cttatgcaac catgcttcca gagcggcaac actgcgtgta atttcttcgt
     1621 gtggaagggg ggcagataat ggctgctgct ccagttgtgc gcatagctgg ctggcgatac
     1681 gcattcccaa actcgagcaa ctgcttttta gctgatgcgc ggcacgcttt attttctcgc
     1741 tatcctgact ggcgcgggca atgtcgattt catcgagaag cggcagggca tgttgtgtaa
     1801 ataataccag ccattcgtgg atcttctccg tccccattaa ctgagcatct tcattgagtt
     1861 gcgatacatc cagcgattga tcgttattga cttgcagttg gagatagtgc gccagtaact
     1921 gaccgagcac ttcacgcggc accggtttag ggataatccc gcggaataat gaactggtac
     1981 gctggcgcag cgtttcgtca atgacatggg cgctaaagcc aatcaaaacc agcgacggat
     2041 attgctgtgc cagttgtcgg gcaagcgtaa tgccgtcgac atccggcaga tcaaaatcca
     2101 ccagtgcggc agcaaacggt tcgctatttt gcagtgtctc taaagcctgc gcggcattgc
     2161 caacagcaac aacctgcgca ccactggtgt tcagcatctc aacggtaatt cgctgggtta
     2221 gcgggttatc ttcaattaac agcaaacgta aaccgtcaag acgcaccgcc tgattgactg
     2281 tttttggcac gggtgccgtg gcaacacgta acggcaagcg taaacaaaaa cagcttccaa
     2341 cctccggcgt gctggtggcg ctcagttcgc cgcccatcgc ctgggccaga cggctactga
     2401 tagtcagtcc cagcccggtg ccgccgcgtt tgccgcttac ctgcacaaat ggctggaaga
     2461 tttctgccag tttcgcggga tcaataccgc agccgctgtc ttccacttcg accagccatt
     2521 gctcgccatc agtgcggcta cgtaaaacga tctgcccttc gtcagtaaaa cgcagggcgt
     2581 tgctcaacag gttggttata acctgacgaa tacgtcgcgg atcgcccatt aacgcggtcg
     2641 gtacatcgtc ggcaattgct gttgccaggc gaatcgggcg acctttcacc cgtccgctca
     2701 ttaattgcag ggtactttcc agcagcgggc gcggttcaaa gggctcatcg ctgaccgaaa
     2761 cattcttgcc acctgcttcg atagccgaat aatcgagaat atcgttgagg atggtcagca
     2821 acgattcgcc agagtcggta atcgcccgca aatcatcacg ctgggcgtta agtgcggggt
     2881 tatctgccag cagttgagca gtgccgagaa taccgtacag cggtgtgcgg atctcatggc
     2941 tcatcgccgc cagaaaggcc gattttgcct ggctggcttt ttctgcttcc gcccgtgcct
     3001 gtcggtgttc tatcaccagt tcctgcaatt cagctgtacg cgctttgacc tgcgccgcca
     3061 gctgttcacg gtggcgattc agtgcatgaa cagtgctgcg aaacgcatcc atcagccgcc
     3121 cgatggtatc cagctcccgt acgccagcgg tttccgggaa aggggagtca atatcaccgt
     3181 ccagcagccg ttgcagcgcc tgcgtttgtt cggcaagtgg acgcgtgact gagcgataaa
     3241 ccacgcgcca aaggatcaga atcagtgcgc aaagtgaaac catccccagc aataacaggc
     3301 tgtattgccc gcgtgcactg gctttttcca gatgcgccag tccgtgctga ttacgcagct
     3361 caatggtgtc gaccagctga ctgacttcgc tactaaactg cgcgaactgg gcgatgttat
     3421 tttgtgcgag agtttgtagg tgattgctga tttcactgtc ctgctgatac agcgccagca
     3481 aatcgctata ttggctaacg gtagttaacg ttgttgcgac ctgcgcacga acacccggat
     3541 cttcaatgcg tatttgccga cgttgcagaa ttttcaccgc attattgagc tgcttttcca
     3601 gcattggtgc atttttctgg atctgctcca gccccagatt catcaccatt tgctgcaccc
     3661 gcagagcgct aaggcgcagt tcattcatct ggttaacata ctcaagatcg atatcaatca
     3721 gccgatcgag tgcactttca gcagcctgac gctgatcttg ttcgatcaaa tcgtaaatcc
     3781 cggcctgggt cgctccagcg gaagttgtcg cattattcgc ctgaccttgc gccaggcgtg
     3841 cgatctcatc ggcggcagcg actatctgct gactgagttg ctgttgttgc tggcgtagtt
     3901 gcagacgccg ccccaccagt tccccttgct gacgtaacga acgggagatc tcctgctcct
     3961 gttgttcaat agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa
     4021 tcttcaggct ttgtgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg
     4081 cactggtcag gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg
     4141 ccatattcat ggtaggaatc aatgcctgag tgttgtcttt ttccacctgg ctgataaagc
     4201 gcaggttgta ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc
     4261 ccatccagag tcttcgggtc agggttaaat tcacggtcgg tgcactttag gtgaaaaagg
     4321 ttgagtcgca aagcagaatg catctagcat aaagccttat tattgattag gctatcatgc
     4381 gcgtactgct atttttactt ctttcccttt tcatgttatc ggcattttcg gctgataacc
     4441 tgttgcgctg gcatgatgcg cagcattaca cggtgcaagc ctctacgccg cttaaagcca
     4501 aacgcgcatg gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt
     4561 tgaactatgg tatgcaggag gcaggcggct acagccagtt ggctacccag caagcacaaa
     4621 tcgaccagtg taaacagtgg ggcgcagagg ccattttgct cggtagtagc actacgtcat
     4681 ttcccgacct gcaaaagcag gtagcaaatc tgccggtgat cgaactggta aatgctcttg
     4741 atgatcccct ggtgaaaagc cgcgttggtg taccctggtt tcagatgggc tatcaacctg
     4801 ggcgatattt ggtgcaatgg gcgcacggta aaccactgaa cgtgctgttg atgcccggac
     4861 ccgataatgc cgggggcagt aaagagatgg tcgagggttt tcgcgcagcc attgccggaa
     4921 gcccggtgcg tattgttgat attgcgcttg gtgataacga tattgaaatc cagcgtaacc
     4981 tgttgcagga gatgctggaa cgccacccag aaatcgacgt cgttgccgga acagccattg
     5041 cggcagaggc ggcaatgggt gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt
     5101 tttatctttc acatcaggtg tatcgcgggc tgaagcgggg aagagtgatt atggctgcca
     5161 gcgatcaaat gatctggcag ggggaactgg cggttgagca ggccatcagg caattacagg
     5221 ggcaatcggt gtctgataat gtcagcccac cgattttagt tctgacgccg aaaaatgccg
     5281 accgtgaaca tattcgccgc tcgctgtcac cagggggatt tcgtccggtc tatttttatc
     5341 agcacacatc agcggctaag aaataacatt caccatgttg cgtcaccagt aaatccgcgc
     5401 tgagtttatg acgtaaacga cgaattaaca catcgacggt gcgcaggtca gggttttcca
     5461 cccgacgcgc agaaagcata cgtagcagac gttcacggct gagaatttcg cccggattcg
     5521 tcacaaatgc caccaacatt tcatactctg cgcgggtcag tttaatcggc tcgccatccc
     5581 gctccagcgt atggcgcgac acattcaggc aataaccggc aaagcgatag cagttgtcct
     5641 gagtgtgcgg ttgagcttgt cgcgcgaggt cgattcgcca gagcagattt ttcacccgta
     5701 ctaccagttc gcgcagttcc agcggtttgg tgacgtaatc gtctgcgccc atttccagcc
     5761 caacaatacg gtcaatccga tcgctgcgtc cggtaaccag aataatcccc accgttgagc
     5821 gttctcgcag ggcgcgggtt aacatcaggc cattttcatc gggtaagttg atatccagca
     5881 gaattaaatc taccggctga gtctgcataa tttcccgcag cccggcaccg ctcgctgtaa
     5941 cggaaacggt atacccctcc tgagtgaagt aagattgtaa tcgcgcctgg gtaaccggct
     6001 catcttcaac aataacaatg tgatgtggca tcagagggtt ttactcattc tgttcatatt
     6061 ctgccgtaag ccgttcatcc tgaccagtgc cgctgttcat atttgctcat taagatcgct
     6121 tcactaaacc ataattttac aggggctatt atgcggaaac tctggaacgc gctacgccga
     6181 cccagtgctc gttggtcggt actggcgctg gtcgcaattg ggattgtgat tggcattgcg
     6241 ctgattgtat tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt
     6301 tgccacagta tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc
     6361 tccggcgtgc gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg
     6421 aagcgcaaac tggaagcgag caatgatatc taccagacct ttattgctca ctccattgat
     6481 acacctgaaa aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg
     6541 aaagaaaaca actcggcaac ctgccgctcc tgccataact acgatgcgat ggatcatgcg
     6601 aagcagcatc ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc
     6661 atcgactgtc ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag
     6721 cagttcgatg agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat
     6781 attaagccaa tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct
     6841 tcggaagtga aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg
     6901 acggaaagcg ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt
     6961 gcctcgattc gtggtgatgt gcagcagcag gtaaaaacgc tggagaaaac caccgttgcc
     7021 gacaccaata ccgagtggag caagttgcag gccactgcgt ggatgaagaa aggtgatatg
     7081 gtgaacgata tcaaaccgat ctgggcttat gcggattcgt tgtacaacgg cacctgtaac
     7141 cagtgccacg gcgcaccgga aatctctcac tttgatgcta acggctggat tggcacgctc
     7201 aacggcatga ttggctttac cagtctcgat aaacgtgaag aacgcacctt gttgaaatat
     7261 ctgcaaatga atgcgtctga catcgcaggt aaggctcacg gcgataagaa ggaagaaaaa
     7321 taatgaacaa taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg
     7381 gcttaacagt cgccgggatg ctggggccgt cattgttaac gccgcgcagt gcgactgcgg
     7441 cgcaagcggc ggatgtcatc tcgaaagagg gcattcttac cgggtcgcac tggggggcta
     7501 tccgcgcgac ggtgaaggat ggtcgctttg tggcggcaaa accgttcgaa ctggataaat
     7561 atccgtcgaa aa
//
LOCUS       DAJTNN010000184         7520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTNN010000184 DAJTNN010000000
VERSION     DAJTNN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.186"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..983)
                     /gene="hscC"
                     /locus_tag="OO150_003552"
     CDS             complement(<1..983)
                     /gene="hscC"
                     /locus_tag="OO150_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR5517565.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEIHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQS"
     gene            complement(1067..2002)
                     /gene="rihA"
                     /locus_tag="OO150_003553"
     CDS             complement(1067..2002)
                     /gene="rihA"
                     /locus_tag="OO150_003553"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5517566.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTDNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2120..2845)
                     /gene="gltL"
                     /locus_tag="OO150_003554"
     CDS             complement(2120..2845)
                     /gene="gltL"
                     /locus_tag="OO150_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5517567.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(2845..3519)
                     /gene="gltK"
                     /locus_tag="OO150_003555"
     CDS             complement(2845..3519)
                     /gene="gltK"
                     /locus_tag="OO150_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5517568.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNIFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(3519..4259)
                     /gene="gltJ"
                     /locus_tag="OO150_003556"
     CDS             complement(3519..4259)
                     /gene="gltJ"
                     /locus_tag="OO150_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5517569.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            complement(4429..5337)
                     /gene="gltI"
                     /locus_tag="OO150_003557"
     CDS             complement(4429..5337)
                     /gene="gltI"
                     /locus_tag="OO150_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5517570.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            5792..>7520
                     /locus_tag="OO150_003558"
     CDS             5792..>7520
                     /locus_tag="OO150_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR5517571.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYV"
BASE COUNT         1895 a         1838 c         1888 g         1899 t
ORIGIN      
        1 taactttgat acggtaattt gccaaacaga cgcacggcga ttcgctgcac cagcggcatt
       61 tgtgacgcgc cgccaaccag caccagactg tcgatttgac tcggcttcag acgcgcatcg
      121 cgcaacgcct gttcaatcgg cactcgcaag cgattgagca gcggcaacca caaatcttcc
      181 agttcgttct cgtaaaattc gcattcccgc gtttcttcct gatactgcca gcgaatgtgc
      241 aatggcgatt gattgctaca ttttgccgct tccacacagg cgtacaaggc tgccagttca
      301 ctctcgttaa gcgtggtcct ggcaacttcc gcgcgtttta aaacctcatc gaccagcata
      361 tgggtaaaat cttcgccacc gaggaagcta tcgccagcgg aggcgtgaat ttcaatcacc
      421 ggcgtggcgt actcaagcac cgtaacgtca aacgtgccgc caccgagatc aaaaaccagc
      481 gagcgggtat tttgttgcgt atgcaggcca tatgccatcg cagctgctgt gggttcatta
      541 attaagcgta ccgcatttaa cccggctaac tccgcagcta aacgggtatg cttgcgttgt
      601 tcatcgctga aataagccgg aacggagatc accacatctt taatcggacg ttgcagaaat
      661 tcttcggcat cttcttttaa tgagcgtaat accaaagagg acagttctgg cgcgttaaat
      721 gtgtcgctgc ctaaccgcca gttggtatta ctgcccattg cgcgtttaaa taacgctgcc
      781 gttttatccg gatgcgaagt acgccgtgat acagccggtt ttccgactaa aatatgatta
      841 ttttcatcca tgctaattat ggatggtgtt aaatattcac cgaacttatt tggaattaat
      901 tgcgcggcac cgtctttcca gacggcaatt aaactattgg tagtgccgag atcgatacca
      961 atggtgagtt ctgcattatc cattgcattt atatcttgaa agaaaaagag ataaacagat
     1021 taagaccacc caggtaacgc gtggtgatct taatcaatga cgtgtgttaa gcgtaaaatt
     1081 tcagacgatc agccagtaaa tcaacaaagc cctgacgatc aacatcgacc attacagtgg
     1141 cattcggttt attgtcagtc agataataat aatcaacaac cgtcataccc tgggtatatt
     1201 tcccctgtgt ttccacgcca acccagcgct caacagtggt aaacaactcg ggtttcaaca
     1261 gccaggcgat ggtgcatggg tcatgcagcg gtgcaccgac aaagccccat ttttcgtctt
     1321 tatgatattc gaggaagaaa tccagcagtt cggcaacaat ggttgaaaca gggttgccaa
     1381 tcgcgcggaa acgctcggtg tcttcaatgt ggatttgcgc tttatgagta acatccagac
     1441 cggccatcac cactgggatc cctgactgga agacaatttc tgccgcttcc gggtcaacgt
     1501 aaatattaaa ttcagccgca ggcgtccagt taccaagccc cattgcgccg cccataatca
     1561 cgatacgggc aattttgcta tgcagttccg gatggctatt gagcagcaag gcaacgttag
     1621 tttgcggtcc ggtagacaca atcgtgacag gttccgcact ttcgcgcagc gttttcgcca
     1681 tcagctctac cgccgtacag ttttgcggtg cgaacgtcgg ttccggtaat gccgggccat
     1741 ccagaccgct ttcgccgtgc acattgtccg cgataatcaa ctcacgcatt aacggtttta
     1801 ccgcgccgcc tgctaccgga atatcggtgc gattaagcaa ggtcagcata cgcagaacat
     1861 tgcgtaaggt tttttctggt gtctggtttc cggcggaaga cgtaattgct ttgacatcaa
     1921 gctctggtga ggcgagggcg agaactattg cgatagcgtc gtcatgacct gggtcgcaat
     1981 ctaacagaat tggcagtgcc attgttgctc cttgttgtgt gcttctttgc gacaagggta
     2041 acgccaggat gtaacagata cgaggggcga aacgataaag cgtgagatgg cgcgcacttg
     2101 ggtatgcgcg ccagagtgat taatgcagga ttttcgcgag gaagtctttc gcgcggtccg
     2161 atttcggatc atcgaagaaa gcgtctttcg gcgagtcttc gacaatttta ccctcgtcca
     2221 taaagatcac ccgattcgcc actttgcggg caaagcccat ttcgtgggtc accaccatca
     2281 tggtcattcc ttcgttcgcc agttccacca tcacgtccag cacttcgttg atcatctccg
     2341 gatccagcgc cgatgtcggt tcgtcgaaca gcatcgcaat aggatccata cacaacgcgc
     2401 gagcgattgc cacacgctgc tgctgaccgc cggaaagctg cgccggaaat ttattggcat
     2461 gtgcggaaag tcccacgcgc tccagcaatt tcaatgcttt ttcacgagcg ggcgctttat
     2521 cgcgtttaag cactttcacc tgcgccagag tcaggttttc aataatcgac aaatggggga
     2581 acaactcaaa atgctggaac accatcccga cgcgggaacg cagctttgcc agatcggttt
     2641 tcttgtcatt aaccacgata ccatcgacgg tgatttcacc ttgctgcact ggttcgaggc
     2701 cgttgacggt tttaatcagc gttgatttgc cggaaccaga cgggccgcaa accaccacca
     2761 cttcgccttt tttcacttcg gttgagcagt cggtcagcac ctgaaagtga ccataccatt
     2821 ttgaaacatt tttcagggta atcattatgc tgtccttctt ttcaagtagc tgaccaacaa
     2881 cgacgcgcta agactaataa cgaaataaac aaatccggca aacaggatca tctcaacctg
     2941 cgtaccatca cgctcaccaa tggttgaggc ggtacggaag aaatcggcca gagataacac
     3001 atacaccagt gaggtatcct ggaacagtac gatgccctga gtgagcagaa gcggcaccat
     3061 cgcgcggaac gcctgcggca gaataatcag tttcatcgac tgccagtgag tcattcccaa
     3121 cgccagcgcg gcgctcgact gaccacgaga aatactttga ataccagcac ggataatctc
     3181 tgaatagtag gccgcttcaa acatcgaaaa cgccaccatc gccgaaatta aacggatatc
     3241 attttttggt gataatccca gcacgttttg cagaaaacct ggcacgatca ggtaaaacca
     3301 cagcaaaacc ataactaaag gaatcgagcg gaatatgtta acgtaggctt tggcaaacca
     3361 cgctacgggc gcaaagctgg acaagcgcat caccgccagc atggtgcccc acaaaatacc
     3421 aatcacgact gccgtgacgg tgattttcag cgtgatcacc agcccgtcga gcagatatgg
     3481 cagggaaggg acaatggaac tccagtcaaa ctcgtacatt atttgccccc catgttgcca
     3541 ggcaggcgaa ctttacgttc aaccagcgtc attaccagca tgataatcgc gttaatcaac
     3601 acatacgcca gcgtaatagc ggtaaacgac tcccaggcgt gggctgagta atcaagcaat
     3661 ttacccgcct gcgccgccat atccaccaga ccaatagtcg aggcgatggc ggagtttttc
     3721 accaggttca tcatctcgga agtcatcggc gggacgataa cgcgataagc attaggcagc
     3781 agtacgtatc gataagcctg cggtagcgtc aggcccatcg ccagcgcggc atttttttgc
     3841 ccacgcggca acgactgaat ggcggcgcgt acctgttcgc aaacacgggc ggcggtaaac
     3901 agccccaggc agagcatgga tgaaaggaaa aactgaatat tgggatccag ctcggcctta
     3961 aaccacatgc cgattttctc cggcaacagc tccgggatca ccagatacca ggtaaagaat
     4021 tgcacaatca acggcacgtt gcggaacagt tcgacataca acgtacccag accggagaga
     4081 aaacggtttg gaacggtacg taaaatgccg aaaaatgaac cgacgaggaa agcgataatc
     4141 caggcgcaga tcgacaaagc gatcgtcacc tgaaaaccac tccagatcca accaagatag
     4201 gtggtgttgc cgaacggggc ttgttgtaaa aaaatacccc agttccagtc tatagacata
     4261 aatctactcc agaaaaaaga gggtagcagc gttaactgct accctcgaag attgttaccc
     4321 agcgtattgc ggttttcagg cccgatgggg aacgaccatc aggcgtatag tctgtccgtg
     4381 ctacgtaaca atcgagaggg ctggaatttc cgcccctggt tcttgtaatt agttcagtgc
     4441 tttgtcattc ggttctttga acagtgcttt catttcgtct gacagttcga aattcatgtt
     4501 caggtttttc ggcggaattg gatttttgaa ccatttatca aaccattttt ccgcttcacc
     4561 ggaagtctgc acctgagcga tagtgtcatc catcagcttt ttgaactgcg gatcatcttt
     4621 acgcaacatg caaccgtagg cttcctgaga ctgcggcttg ccgacgatat cccagttgtc
     4681 tggtttcttc gctttcgcac gttcaccggc cagcagggca tcatccatca taaaggcaac
     4741 ggcacgaccg ctttccaggg tgcggaaaga gtcaccgtga tctttggcgc tgatgatgcg
     4801 catattcatt ttttgctctt cattcagttt gttgagcaaa acttcagagg tagtgccgga
     4861 agtgacgact acggctttgt ctttcaggtc ggcaaaatct ttgatatcgc cgcccttttt
     4921 ggtcagcagg cgcgtaccga ccacgaaaat agtatcagag aaagccgcct gtttttggcg
     4981 ttcgacgttg ttggtggtag aaccacattc aaaatcgaaa gtgccgtttt gcagcagtgg
     5041 aatacggttt tgtgaggtaa tcggaatcag ttttacctgc aagtccggtt tgttgagttt
     5101 ctttttcact gcttcaacaa tggcgttgga gtaatcctgc gagtaaccca ccactttttg
     5161 ctgattgtcg taataagaga aaggtactga agattcacgg tgaccgacga caatcacacc
     5221 gtttttggcg attttgtcca gcgtactgcc tgctgccggg gcggcgtcat ctgcctgtgc
     5281 cagtcctgcg gaaagcgcca gggcgaggat tgctgtggca ggtttacgta attgcatatc
     5341 caactccttt atcttctgcg ttaagaacgc atggataccc gttgtgagtg tttgtgttgt
     5401 tatcgtctgc aactttattg tgcagtgttg tgcctgttag gtaacattta gtttggctaa
     5461 atgtaaagat attgctgttt tattgtttgt ttttgcgaga tgcgccgcac cattccgaag
     5521 caaaattctt aaaccgcacc tttttggtgc taccgctgat ttgtggtggt gcaacctggt
     5581 tgcgtcgcgg ctgatttcat cgcttacaca gtaaataaag caacttacat gccaaaaaat
     5641 acttaggggc aaattaatta cgattgtaat tttaggctga aaaagtcata tttcggacag
     5701 aattatcttt tcggcatggg acgtttgccg ggatgataac tgcacacggg aaaaatgctt
     5761 tttacctgtc ttttcaagga agaaacacct tatgtctgca tcttcggtta agccgttaaa
     5821 tgttcaatta cccgcaataa cccttatcct ttttgcactc tgtattggga tattttgtta
     5881 tctcgcacaa tggatgagtt atgaagaggt cgatcaatcc gcacttatcc atctcggcgc
     5941 aaacgttgcg cctcttactt tgtcgggtga accctggcgc ttattgagca gtatctttct
     6001 gcacagtagt gtttctcatc tgctgatgaa tatgtttgca ttcctggtcg tgggaggcgt
     6061 ggcggaacag attctgggga aatggcgact cctgattacc tggttattct ccggcgtctt
     6121 tggtgggctc atcagcgcct gttatgcgtt acgcgatagt gatcagatag tcatcagcgt
     6181 tggggcatcc ggggcaatta tgggaatagc tggcgctgcg atagcaacac agcttgcttc
     6241 aggtgcgggc acataccata aaaaccagcg gcgagtattt tctctgttgg gtatggtggc
     6301 gctgacactg ttgtatggtg cccggcaaac aggaatagat aatgcttgtc acattggcgg
     6361 cctgattgcg ggtggcgcgt tgggttggct gagcgcgcgt ttatctgggc aaaaccgact
     6421 cgttacggaa ggcgggatta ttgttgcggg cagtcttctt ctgaccgggg ctatctggct
     6481 tgcgcagcag cagatggatg agtcagtttt acaagtcagg caaagcctgc gtgaagcgtt
     6541 ttatccacag gagattgaac aagagcgacg gcaaaaaaag cagcagttag cggaggaacg
     6601 caacgccctc agggaaacat tatccgctcc ggtaagtcgt gaacaggcca gtggtgattt
     6661 gctcgctgag attgccgata tccatgatat ggcgatcagt cgggatggta atatgttgta
     6721 tgccgcaatt gaaaacacga acagcattgt tgttttcgac ctcggacaaa agaaaatcct
     6781 gcataccttt acagcgccca tagcgaaaga aaagtcagtc aaacattgtg gtggctgtaa
     6841 agatcagggc gtcagatcgc tggcattaag cccggatgaa aagttgattt atgcgacttc
     6901 atttgaagcg aatgcgttat cggtcattaa cgtggcgaca ggggagatta ttcagtcgat
     6961 taccaccggt gcacatcctg acagccttat cctctcgcgt gatggcacaa aagcctgggt
     7021 gatgaatcgc accagtaata gtgtgtcagc gattgatctg gtgacttatc agcatgtggc
     7081 ggatatcccg ctggagaaat acgacggggc ggggacgagc ggtaaaccag gcgcctgggt
     7141 tatggcactt tccccggatg aaagaacatt actggttccg ggagcaggca gaggtaacat
     7201 cgtgcggatc aataccatca cgcatcagaa agaagacttt cccgcaggtg atgcgcgtgg
     7261 aacgatatcg gcgatgcgtt ttcgacctga aaacggcgag gttatttttg cagatagtca
     7321 ggggatttca cgtataagcg taggggatca acaagccagc attatgacgc aatggtgtag
     7381 caggagcgtt tattccgttg agggtattag cccggacggt caatatttag cgttggtgtc
     7441 atatggcctg caaggttatg tcatcctgct caatattaat gccgggcaga ttattggcgt
     7501 ttatcctgcc agctacgtta
//
LOCUS       DAJTNN010000185         7472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTNN010000185 DAJTNN010000000
VERSION     DAJTNN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.187"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..339
                     /locus_tag="OO150_003559"
     CDS             <1..339
                     /locus_tag="OO150_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5517572.1"
                     /translation="QTEAFLNAYGLSRFAPLGYDPRDLPIRDLAGYRKKGNHDGDPII
                     FYTFPAAFEQEIAKGFNTKQFAEVLKNAGMLTPPTSGRGYQGRVREDGRQIRVYVLNF
                     MAEESSQPEE"
     gene            714..1148
                     /locus_tag="OO150_003560"
     CDS             714..1148
                     /locus_tag="OO150_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR5517573.1"
                     /translation="MTAQISAYGRLVADPQTRTTTNGNNMAMARLAVSLPCNAAEAGE
                     STFWLGVIAFGKQADALAKHQKGDLVSVAGNMQLNQWTGQDGGTQQGYQVVADSVISA
                     RTVRPGGKAGQRGQATDALRRAQQPSRDEYDQRPPFDDETPF"
     gene            1159..1800
                     /locus_tag="OO150_003561"
     CDS             1159..1800
                     /locus_tag="OO150_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517574.1"
                     /translation="MSTQPIIIEFGSYRWPRHLAIHEADHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWHANYPRNVAEVKSYTLSNDDTRLRLIDQVCRAMVIS
                     LAGIVAQSRYTGDDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEAL
                     IALKWWDVIALAKILENRNHMPASNFQRIMGDIDKPVPKPMTLAELDELVGEE"
     gene            1797..2147
                     /locus_tag="OO150_003562"
     CDS             1797..2147
                     /locus_tag="OO150_003562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517575.1"
                     /translation="MKPYILAAGLISSAPALSAIHCGGFTIQPDRYRTTINGDVVHIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRIETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            2841..3452
                     /locus_tag="OO150_003563"
     CDS             2841..3452
                     /locus_tag="OO150_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517576.1"
                     /translation="MRDRTRSYTTDLPRIGLPFLANMRSRLADAEPGTQLYTQTESGT
                     LYAFRQADSYAMTINGVTRAIRTTTTKAGYGVREWYACPHCMKRAAKLYIGKKDIGCR
                     ACWKLHYKSQSADILDRMRMKIRQQRCAIWGSNDLANNLFNDIRMFPKPKGMRWATFD
                     RKRAELSVMEMAYWQAFSPVVDRITGVIERKAGKAARGIGLTL"
     gene            3990..4532
                     /locus_tag="OO150_003564"
     CDS             3990..4532
                     /locus_tag="OO150_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCR5517577.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSSINDRLQTLN"
     gene            complement(4525..4884)
                     /locus_tag="OO150_003565"
     CDS             complement(4525..4884)
                     /locus_tag="OO150_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517578.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            5318..6142
                     /gene="dinD"
                     /locus_tag="OO150_003566"
     CDS             5318..6142
                     /gene="dinD"
                     /locus_tag="OO150_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5517579.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            6434..7051
                     /locus_tag="OO150_003567"
     CDS             6434..7051
                     /locus_tag="OO150_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5517580.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7048..7472)
                     /gene="ligB"
                     /locus_tag="OO150_003568"
                     /gene_synonym="yicF"
                     /pseudo
     CDS             complement(7048..7472)
                     /gene="ligB"
                     /locus_tag="OO150_003568"
                     /gene_synonym="yicF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
BASE COUNT         2000 a         1834 c         1907 g         1731 t
ORIGIN      
        1 cagacggagg cgtttttgaa tgcctatggg ctgagtcgat tcgcgccgct gggttatgac
       61 ccgcgcgatt tacccatccg tgatttagcc gggtacagga agaaggggaa ccacgacggc
      121 gacccgataa ttttctacac cttcccggca gcgtttgagc aggagattgc aaagggcttc
      181 aataccaaac agtttgccga agttctaaag aatgcaggaa tgcttacacc accaacgagc
      241 gggcgaggct atcaggggcg agtaagggag gatgggcgac agattcgcgt ctatgtactc
      301 aatttcatgg cagaggaaag cagccagcca gaagaataat tatttcttca catgcgtagt
      361 tttagtgttg tctcggttgt ctttgttgtc tcaatttgta attaagattg atttataagg
      421 attttatttt aatttctgag acaacaccga gacaacattt agccattttg agacaacagg
      481 gcgaaagtgt gtttttagtc tggcaggcag tgagacaaca tttatgccgg gtgagacaac
      541 acgaaattgc aaatgttgtc tcagcaaaac ccagacgtgg cgcggcctgt agagccgtga
      601 gacaacaaag acaacagaga caacactatt tttgcttact atgaaaaaaa atcggcaggt
      661 gtcccacatt aagaaatcgt cattagcaaa actaataatc cagtgagggg aaaatgacag
      721 cacaaatttc agcatacggg cggctggtgg cagatcccca gacccgcacc acaacgaacg
      781 gtaacaacat ggctatggcg cggctggcgg tgtctctccc ctgtaatgcg gcagaggcgg
      841 gagagtcaac cttctggctg ggcgttattg cttttgggaa gcaggccgat gcgctggcaa
      901 agcaccagaa aggcgacctt gtgagcgtgg caggcaatat gcaactcaac cagtggacag
      961 ggcaggacgg cggtacacag caggggtatc aggtagtagc cgacagtgtg atcagcgcca
     1021 gaacggtacg ccccggcggg aaggcaggac agcggggaca ggcaacggac gcgctgagac
     1081 gtgcgcagca gccatcccgt gatgaatacg accaacgccc accattcgac gatgaaacgc
     1141 cattctgaga gggaagatat gagtacgcaa cccattatta ttgagttcgg cagctatcgc
     1201 tggcccagac atcttgcaat acatgaagca gatcatgcga tagccgcatg gtttttcggc
     1261 atacaggagg tagaaattgc gttatcgggt aataaccgag tggcaaaaac ctaccttagc
     1321 ggcgatgttg atgaatgtgg agctgtcgcg gtatggcacg ccaactatcc acggaatgtg
     1381 gctgaggtta agtcttacac cctctcgaat gatgacacca ggttacgtct gatagaccag
     1441 gtatgccggg ctatggttat ctctctggct ggtatcgtcg cgcaatcacg ctatacgggg
     1501 gatgatatta acgcactgat ggaaacgtcc gggaaagcag acatgaaaaa tgttcgtgat
     1561 ttatcagagg tataccgggt ggctggtggg gaggatgctg atatacagag aatatccctt
     1621 tcccgtgctg aggcgctgat tgccctgaaa tggtgggatg ttatcgcgct ggcgaaaata
     1681 ctggagaacc gtaaccatat gcctgccagc aacttccagc gcattatggg tgatattgac
     1741 aagcctgtac caaagcctat gacgctggct gaactggatg aattggtggg tgaagaatga
     1801 agccatacat tctggcagcc ggcctgatct cctcggcacc ggcactatct gctatccatt
     1861 gtgggggctt taccatacag ccagaccgtt atcggacaac gattaacggc gatgtggttc
     1921 atattgttga tgcgcaattt tacggcgcac cgcgagactt tagccaggcc gccataaagt
     1981 tattgccgga tgccattacc actaccgata ggcaataccg catagagacg cagggaggca
     2041 atgccttgct tgagttgatg accagagaaa acccgccacg tgtgcttaat cgtgagcgct
     2101 gcgatgccgg gttaacaagt ttcggttggt acacttctgc gccgtgatgt tcgttccgag
     2161 cgcagcctaa aatattggac tgttcttgac ggatttaaaa ctctaatgga gccaagtcct
     2221 tgccagtagc gggctaggag cctaattgtg attcaggtcg tcaaaaatat tcaggtttgc
     2281 ggcattggtt cgcagtacgc agtccggtac gcggcaaaac tgcgcattta acgtgcgtac
     2341 cacctgacga attctaacgc cggcattgca aacagatgcg tgcgcaaaat ggctaagacg
     2401 tctgatattg ctggctttgg ctcaatcagt ttggaggtgg cggtatcagt acgatgctaa
     2461 tttttgctaa ggttttagta cgcaactcag gtacgcactc tcaccagata cgaaaccgat
     2521 tcgcagttgc gtaccagcct gtaaggcgca ttatcgctgg gtttctacag tcgaatgcag
     2581 tacgcaaagt ggaaaatcct ccgaatacga aacccgagca ttgcgcagta aattgcgaat
     2641 tgcgcagtta acaaaatatc tccgcaaaac gaaacggata tcaatgcgca aaccatcctc
     2701 aacccccgcg cggttactgg ctttattgat gtgaaattgc aaaattggtg cgcaatttac
     2761 agtaaaatac gcatattatt tcgtattcaa tttgcgcacc gttcgcagtt aatgcgcagt
     2821 aattcgcagg aggccagcca gtgagagaca gaacgcgcag ctataccacc gacctgcccc
     2881 gtatagggtt gccattcctt gcgaacatgc gtagcaggct ggcggatgct gaaccgggta
     2941 cgcagcttta tacccagacc gaaagcggaa cgttgtatgc cttccggcaa gccgacagct
     3001 acgccatgac aatcaacggc gtgacgcggg ctattcgcac cacaaccacg aaggccgggt
     3061 atggcgtccg ggaatggtac gcttgccccc actgtatgaa gcgggctgca aagctgtata
     3121 tcggcaagaa agacattggc tgccgggcgt gctggaaact tcactataaa agccagagtg
     3181 ccgacatact ggacaggatg cgcatgaaga tacgccagca gcgatgcgcg atatggggca
     3241 gcaatgacct tgccaataat ctgtttaatg acattcgcat gttcccaaaa ccaaagggga
     3301 tgcgctgggc tacgtttgac aggaagcggg cagagttatc agttatggag atggcttact
     3361 ggcaggcatt cagcccggta gtggacagaa tcacgggcgt tattgagcgt aaagcaggaa
     3421 aagcagccag aggcattggc ctgactctat aaaagcaaaa cgcccgcacg gggcgtcaga
     3481 gaacgggata gacttctgcg acaatttgca ttcgccgcca atatagcaag ccgtccacat
     3541 caagcaataa actatcaccg ggagggggag gccaaatctc tgcaatcctg agccttaagg
     3601 actgcccgcc ccatcgtttt ttcatacccg cgaaaaatga aatttaatcc gggcgtgttt
     3661 catccttcca gagggtatga cgtatgacac cacgcgaaat agccttattg accatcgcca
     3721 aacttgagca tggaggccac cagcttacac aggcagatca acgggagata gaacgctcag
     3781 ttaatgccga tatagcccgg cgcgacaggt tccgcgaaat gatgcgagca cctgcctacc
     3841 agaggaagaa gccagcgccg cgcaggtaga tacgggatta ttccgcatgt aacttatgcg
     3901 tattaagcaa atttccttta ttcaccttta cagaattgtg agtacgtcag tatcgttatc
     3961 cttccgattc gcatttattc aggatttgca tgtaccagct aaagataacc atccgagaca
     4021 gcaaaccacc tatatggcgg cgtgtgctgg ttccagagca aatccccttt agtgaacttc
     4081 acgcggtgat ccagttggct tttggctgga atgacgaaca cctgtatatg ttcgagaaag
     4141 ggcgtaaagg tgatcccggt agcgagtatc gcgtatgggg tgaggatgaa agcatgggta
     4201 atgcggcaat cacaccacta tgggcagcgc tccagaatga tggtgacaag ctggtttaca
     4261 cgtatgactt tggcgactgg tgggattgcg tcattgtgct ggagaagcaa acacacgata
     4321 cgagcaatca gcccattagc tgcctgcgtg ggaaaggcac caccccggtg gaaaactcag
     4381 gcggcttgca tggttacaat gaattgctat tacaggccag agagtctgat aatcctgagc
     4441 aggccgagat ccacaatttc ctgatgctgg atattgagcg ccgggtttac gacctgagca
     4501 gcatcaacga cagattgcag actcttaact gacccgcata tattcgcctg atattgcgtt
     4561 atcagcgccg ccagtatcgc gcataacagc gcactgaccg ggcaaaccgt agcgcacgac
     4621 cgttgcgtta gtctcaccgc cgcgccgtat ctggcgcgat tcatacactg tcacccgctc
     4681 aagcagtcca ccagcaacca tgctttccag tgtgcgccgg gttgattcga gctggtgacg
     4741 cttatcgaac gacaccatgc catgaagcag gtaggccaca cccgacacat cgaacggcgg
     4801 cgcaccaatc tcaccagtca cccattcgag gttatccggt tcaaagtagc taagtatctc
     4861 ttttttgcgg ctggtcattc tcatagctgg caggttcctt attgtgggat agcactatca
     4921 tacaataagg cgcaaaagat aagaggccag aacagcaagc atgagagcat aaaacgagca
     4981 gccaaagaca gcgcctcaaa ataccgtgta cacaagggtg tacctactga gaaaacgcat
     5041 tacaagaaac acagcaacca cgggcgttta aagccactac tcaataaaca gattcaactc
     5101 gtaacgccaa ttcttacttt tcagccttca caaatgccgc ccactcaaac agagcggcat
     5161 ttttcttccc cgcaacttca attctgtttt gcgtgcctgc tccagatttt gcgatgtttt
     5221 tttgcccagc acactgagaa cgtgagatac tcacaactgt atataaatac agttacagat
     5281 ttactttctt tgcaatcgat atcacatgga gtgggcaatg aacgaacatc atcaaccttt
     5341 tgaagaaata cggcattatg gcactgaagg gcaagaattt tggtcagcta gagaattggc
     5401 accgttactg gattatcggg actggcgcaa ttttcaaaag gtactggccc gcgcgactca
     5461 agcgtgcgaa gccagcaatc aagctgcatc tgaccatttc gttgaaacca ccaaaatggt
     5521 tgtattaggt tcaggggctc aacgagaact tgaagatgtt catctctccc gctatgcctg
     5581 ttacctggta gtacaaaacg gcgaccctgc gaaaccggtt attgcggcag ggcaaactta
     5641 ttttgctatc cagacccgac ggcaggagct ggctgacgat gaagctttca ggcaacttcg
     5701 tgaagacgaa aagcgtcttt ttctgagaaa tgaattgaaa gaacataaca aacaattggt
     5761 tgaggcagcg cagcaagctg gggtagcgac agctatcgat ttcgccatat ttcagaatca
     5821 tggttaccgg gggctgtatg gtggattaga tcagaaagct attcatcagc ggaaggggct
     5881 gaaaaagaat cagaatatcc tggatcatat gggttcaaca gaactggcgg ctaatctctt
     5941 tcgagctacc caaacagaag aaaaactcaa gcgggatggc gtgaattcaa aacagcaagc
     6001 aaacaccaca cactttgacg tgggtcgcaa ggtgaggcaa accattcagg aacttggcgg
     6061 aaccatgcct gaggagttgc cgaccccgca ggtcagcatc aagcagttgg aaaacagtgt
     6121 aaaaattaca gagaagaaat agcagaaact aaaaactctt tttcttaatt gagatacccg
     6181 atgcgtaagc caaagtccag gtgcagtgaa cacaatggct aaacattgca cctttctttt
     6241 ccacccctca attttaacct attttttctt atgcattttc tcagacaaga actcagaata
     6301 atgcatctac gctacagaaa atagcgattt cacataacta caagttatca atttcctctt
     6361 accttaaaaa atctcaatcg tgacaatgcg cacaaatcgc taccctgcca gacagatttt
     6421 tagggagaga accatgctgt tacacatttt gtatttggtt ggcatcactg ccgaagccat
     6481 gacgggggcg ctggcggccg gacgacggcg catggataca tttggcgtaa ttattattgc
     6541 gaccgccacc gcaattggcg gagggtcagt gcgcgatatt ctgctgggcc actatccgct
     6601 cggctgggtc aaacacccgg aatatgtgat tatcgtcgct accgccgcag tactcaccac
     6661 tatcgttgct cctgtaatgc cttacctgcg caaagtgttt ctggtgctcg atgcgctcgg
     6721 actggtggtc ttttctatca tcggcgcaca ggttgcactg gatatggggc acggcccaat
     6781 tattgccgtt gtcgcggcgg taaccaccgg cgtgtttggc ggcgttttgc gcgatatgtt
     6841 ctgtaaacgc atcccactgg tattccagaa agagttgtat gctggtgtct catttgcctc
     6901 cgccgtgctg tacatcgcgc tgcaacacta tgtttctaac catgatgtgg tgattatctc
     6961 caccctggta ttcggctttt ttgcccgctt actggcgcta cgtcttaagc tgggattacc
     7021 ggtcttttac tacagccacg aaggtcacta aggttcaaaa cctgtgatct gctgggcagc
     7081 cagccaactg cccagcttct tgatttgcgc attttccttc cattcaataa cctgtctggc
     7141 gcgcccagat ccagtccccg gcagctgctg tcagaactgc tccgtgctaa ataaaagttg
     7201 cgactaggac cgttcatcac tggcattaag cgccgcccgg gttagcggta ttcccattgc
     7261 catcacccag cgagtaaaag gctgcttacg agccagatta aactgatgcc ataactgcgc
     7321 acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc tctggcgtta ataaaagcca
     7381 agaaaagata tgctcaaagc gatgagtctg atgcagcgcg cgccaaccgg cctcaccaat
     7441 gccatccagc ccaagaacct gttttgaccc ca
//
LOCUS       DAJTNN010000186         7428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTNN010000186 DAJTNN010000000
VERSION     DAJTNN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.188"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            157..765
                     /locus_tag="OO150_003569"
     CDS             157..765
                     /locus_tag="OO150_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5517581.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            863..2254
                     /gene="selA"
                     /locus_tag="OO150_003570"
     CDS             863..2254
                     /gene="selA"
                     /locus_tag="OO150_003570"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5517582.1"
                     /translation="MTTETRSLYSQLPAIDRLLHDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2251..4095
                     /gene="selB"
                     /locus_tag="OO150_003571"
     CDS             2251..4095
                     /gene="selB"
                     /locus_tag="OO150_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5517583.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFSDEPWW
                     VRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            4285..5436
                     /gene="yiaY"
                     /locus_tag="OO150_003572"
     CDS             4285..5436
                     /gene="yiaY"
                     /locus_tag="OO150_003572"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5517584.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            5601..7139
                     /gene="aldB"
                     /locus_tag="OO150_003573"
     CDS             5601..7139
                     /gene="aldB"
                     /locus_tag="OO150_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5517585.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT         1764 a         1846 c         2078 g         1740 t
ORIGIN      
        1 aagtgagata tccatcagag tgaaataatt aggaaaatat ttatatcacc atgataaata
       61 tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc catcaccagg
      121 ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg tagctacacc
      181 agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac tttcgaattt
      241 attaatgaac tgccctataa cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa
      301 gtgccggtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat catcgctgaa
      361 tatattgagt taatgaatgt cgctccggcg atgttgccgc gcgatccgct ggagtcgttg
      421 cgggtgcgca aaattgaggc gctggcggat ggcattatgg atgccgggct ggtatcggtg
      481 cgtgaacagg cgcgtccggc ggcgcagcag tctgaagatg aattgttacg ccagcgggag
      541 aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac actcaaaacc
      601 gatacggtca atctggcgac tatcgccatt gcctgtgctg tcggatatct caatttccgc
      661 cgcgttgcgc caggttggtg tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg
      721 tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct
      781 cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc atgtgagagg
      841 gccttgatta gccaggtttc ctatgacaac cgaaacgcgt tccctctata gtcaacttcc
      901 ggctattgat cgcttattgc acgatagctc cttcctttct ttgcgtgata cttatggtca
      961 cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtga
     1021 cagccagacg cttcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac
     1081 gaaagaggcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg tgctgcatac
     1141 caaccttgga cgagctttac aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg
     1201 ttcgccagtg accctcgagt acgatctgga cgacgctgga cgcggacatc gcgatcgggc
     1261 gctggcgcaa ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg tcaataacaa
     1321 tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg tggtagtatc
     1381 tcgcggcgaa ttggtggaga ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc
     1441 aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg attatcgtca
     1501 ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact acagcattca
     1561 ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag agctggatgt
     1621 tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt acggtttgcc
     1681 gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga gcttctccgg
     1741 cgacaagttg ttaggcgggc cgcaggcagg gattatcgtt ggtaaaaaag agatgatcgc
     1801 ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc
     1861 gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa aattaccgac
     1921 cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac gtttacaggc
     1981 tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat gtctttcgca
     2041 gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa cgtttacacc
     2101 ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg aattgccagt
     2161 gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct gccttgaaga
     2221 tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg acacgttgac
     2281 cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg tctgccggaa
     2341 gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca gccggatggt
     2401 cgcgtgccta gttttatcga cgtacccggt catgaaaagt ttctttccaa catgctggcg
     2461 ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggcg
     2521 cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct gacagtggcg
     2581 ctgaccaagg ccgatcgcgt ggacgaagcg cgtgttgatg aggttgaacg ccaggtaaag
     2641 gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa
     2701 ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt taccaggacg tgaacacgcc
     2761 agccaacata gtttccgcct cgcgatcgac cgcgcattta ccgtaaaagg tgccgggctg
     2821 gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact ctggctgact
     2881 ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcac aaaaccagcc aacagaaacc
     2941 gcccatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt
     3001 aaccgtggcg actggctgct tgccgatgcg ccgccagagc cgttcacacg ggtgattgtc
     3061 gagcttcaaa cccatacacc gctgacccag tggcagccgc tgcatattca ccacgccgcc
     3121 agccacgtca cgggacgcgt ttcactgctg gaagataacc ttgctgaact ggtcttcgac
     3181 accccgttat ggctggcaga taacgaccgc ctggtattgc gcgatatctc tgcccgcaac
     3241 acgctggccg gagcgcgcgt cgtgatgctt aacccgccgc gtcgcggtaa acgtaaacca
     3301 gagtatctgc aatggctggc ttccctcgcc agggcacaga atgatagcga agcgttagcc
     3361 gttcacctgc aacgcggcgc ggttaatctt cctgatttcg cctgggcgcg ccagctcaac
     3421 ggcgaaggga tgcgcgaact gctgcaacag cctggctata ttcaggctgg ttatagcttg
     3481 ttgaatgcgc cggttgccgc tcgctggcaa cggaaaattc tcgacacatt agcgacttat
     3541 catgagcaac atcgcgatga acctgggcct gggcgcgaac gtctgcgacg tatggcgttg
     3601 ccaatggaag atgaagcgct agtactgttg ctgattgaaa agatgcgcga aagcggcgac
     3661 atcctcagtc atcacggttg gctgcatctg ccggatcaca aagcgggctt cagcgaagag
     3721 cagcaggcca tctggcaaaa agcagagccg ctgtttagtg acgagccgtg gtgggtgcgt
     3781 gatctggcaa aagagacggg taccgacgag caggcaatgc gcttgacgct aagccaggcg
     3841 gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt
     3901 gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg
     3961 gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct ggaatatttt
     4021 gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga cgcattatta
     4081 tttccggaaa aataaggaaa tgattaatta atttttaaaa taaattaata caaatttctt
     4141 atgattttaa aaaaagcaca ttgtttaatg aatacaatgt gctttttatt agattaattt
     4201 tgataattaa taataatgat catgttttcg aatgaaaatc ctcagtaagc ttcccaccct
     4261 tttttacact ttcaggagtg tgttatggca tcttcaactt tctttattcc ttctgtgaat
     4321 gtcatcggcg ctgattcatt gactgatgca atgaatatga tggcagatta tggatttacc
     4381 cgtaccttaa ttgtcactga cagtatgtta acgaaattag gtatggcggg cgatgtgcaa
     4441 aaagcactgg aagaacgcaa tatttttagc gttatttatg atggcaccca acctaacccc
     4501 accacggaaa acgtcgccgc aggtttgaaa ttacttaaag agaataattg cgatagcgtg
     4561 atctccttag gcggtggttc tccacacgac tgcgcaaaag gtattgcgct ggtggcagcc
     4621 aatggcggcg atattcgcga ttacgaaggc gttgaccgct ctgcaaaacc gcagctgccg
     4681 atgatcgcca tcaacaccac tgcgggtacg gcctctgaaa tgacccgttt ctgcatcatc
     4741 actgacgaag cgcgtcatat caaaatggcg attgttgata aacatgtcac tccgctgctt
     4801 tctgtcaatg actcctctct gatgattggt atgccgaagt cactgaccgc cgcaacgggt
     4861 atggatgcct taacgcacgc tatcgaagca tatgtttcta ttgccgccac gccgatcact
     4921 gacgcttgtg cactgaaagc cgtgaccatg attgccgaaa acctgccgtt agccgttgaa
     4981 gatggcagta atgcgaaagc gcgtgaagca atggcttatg cccagttcct tgccggtatg
     5041 gcgttcaata atgcttctct gggttatgtt catgcgatgg cgtaccagct gggcggtttc
     5101 tacaacctgc cacacggtgt atgtaacgcc gttctgctgc cccatgttca ggtattcaac
     5161 agcaaagtcg ccgctgcacg tctgcgtgac tgtgccgctg caatgggcgt gaacgtgaca
     5221 ggtaaaaacg acgcggaagg tgctgaagcc tgcattaacg ccatccgtga actggcgaag
     5281 aaagtggata tcccggcagg cctacgcgac ctgaacgtga aagaagaaga tttcgcggtt
     5341 ctggcgacta atgccctgaa agatgcctgt ggttttacta acccgatcca ggcaactcac
     5401 gaagaaattg tggcgattta tcgcgcagcg atgtaatcat catttccaca acggctggca
     5461 aattgtcagc cgctttttca actatctctg taacccttgc ccgtaaattc gtgatagccg
     5521 tcgtaaagct gttaccgact ggcgaagatt tcgccagtca cgtctaccct tgttatacct
     5581 cacaccgcaa ggagacgatc atgaccaata atcccccttc agcacagatt aagcccggcg
     5641 agtatggttt ccccctcaag ttaaaagccc gctatgacaa ctttattggc ggcgaatggg
     5701 tagcccctgc cgatggtgag tattaccaga acctgacgcc ggtgactggg cagctgctgt
     5761 gcgaagtggc gtcttcgggc aaacgagaca tcgatctggc gctagatgct gcgcacaaag
     5821 tgaaagataa atgggcgcac acctcggtgc aggatcgcgc ggcgattctg tttaagattg
     5881 ccgatcgaat ggaacaaaac ctcgagctgt tagcgacagc tgaaacctgg gataacggca
     5941 aacccattcg cgaaaccagt gctgccgacg tgccgctggc gattgatcat tttcgctatt
     6001 tcgcctcgtg tattcgggcg caggaaggtg ggatcagtga agttgatagc gaaaccgtgg
     6061 cctatcattt ccatgaaccg ttaggcgtgg tggggcagat tatcccgtgg aacttcccgc
     6121 tgctgatggc gagctggaaa atggctcccg cgctggcggc gggcaactgt gtggtgctga
     6181 aacccgcacg tcttaccccg ctttctgtac tgctgctaat ggaaattgtc ggtgatttac
     6241 tgccgccggg cgtggtgaac gtggtcaatg gcgcaggtgg ggaaattggc gaatatctgg
     6301 cgacctcgaa acgcatcgcc aaagtagcgt ttaccggctc aacggaagtg ggccaacaaa
     6361 ttatgcaata cgcaacgcaa aacattattc cggtgacgct ggagttgggc ggtaagtcgc
     6421 caaatatctt ctttgctgat gtgatggatg aagaagatgc ctttttcgat aaagcgctgg
     6481 aaggctttgc actgtttgcc tttaaccagg gcgaagtttg cacctgtccg agtcgtgctt
     6541 tagtgcagga atctatctac gaacgcttta tggaacgcgc catccgccgt gtcgaaagca
     6601 ttcgtagcgg taacccgctc gacagcgtga cgcaaatggg cgcgcaggtt tctcacgggc
     6661 aactggaaac catcctcaac tacattgata tcggtaaaaa agagggcgct gacgtgctca
     6721 caggcgggcg gcgcaagctg ctggaaggtg aactgaaaga cggctactac ctcgaaccga
     6781 cgattctgtt tggtcagaac aatatgcggg tgttccagga ggagattttt ggcccggtgc
     6841 tggcggtgac caccttcaaa acgatggaag aagcgctgga gctggcgaac gatacgcaat
     6901 atggcctggg cgcgggcgtc tggagccgca acggtaatct ggcctataag atggggcgcg
     6961 gcatacaggc tgggcgcgtg tggaccaact gttatcacgc ttacccggca catgcggcgt
     7021 ttggtggcta caaacaatca ggtatcggtc gcgaaaccca caagatgatg ctggagcatt
     7081 accagcaaac caagtgcctg ctggtgagct actcggataa accgttgggg ctgttctgat
     7141 ataagaagct ggtcgcattg ggtattcatt gcctgatgtg acgcttacgc gtcttatcat
     7201 gcctacggga acctgaccgt aggccggata aggcgtacac gccgcatccg gcataaacaa
     7261 agcgcacgtt gttacaattt acgttttaaa cctacgtgcg caataatcca gactacgcca
     7321 acagaagtgc gtttctgggg cgtgttttgt caggagttta atctcgcgtt tcgatggtga
     7381 gcgggcgaac taggccgtaa atcaccattg gtgatccgag gccaatca
//
LOCUS       DAJTNN010000187         7391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTNN010000187 DAJTNN010000000
VERSION     DAJTNN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.189"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..1459
                     /locus_tag="OO150_003574"
                     /pseudo
     CDS             1..1459
                     /locus_tag="OO150_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1470..2543
                     /locus_tag="OO150_003575"
     CDS             1470..2543
                     /locus_tag="OO150_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5517586.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVITHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2791..3831
                     /gene="pstS"
                     /locus_tag="OO150_003576"
     CDS             2791..3831
                     /gene="pstS"
                     /locus_tag="OO150_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5517587.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3918..4877
                     /gene="pstC"
                     /locus_tag="OO150_003577"
     CDS             3918..4877
                     /gene="pstC"
                     /locus_tag="OO150_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5517588.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4877..5767
                     /gene="pstA"
                     /locus_tag="OO150_003578"
     CDS             4877..5767
                     /gene="pstA"
                     /locus_tag="OO150_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5517589.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5858..6631
                     /gene="pstB"
                     /locus_tag="OO150_003579"
     CDS             5858..6631
                     /gene="pstB"
                     /locus_tag="OO150_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5517590.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            6646..7371
                     /gene="phoU"
                     /locus_tag="OO150_003580"
     CDS             6646..7371
                     /gene="phoU"
                     /locus_tag="OO150_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5517591.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1856 a         1718 c         1931 g         1886 t
ORIGIN      
        1 cagatgttga tgtcagcgtg ggtgaattgc gcgatgaaga cggatttaca ccggacgtcc
       61 ttcaggcgca aatacttcat ggtttttccc acgggatcac tttatatgga ggtatgcagg
      121 ctgctgaaaa ttatggttct gcagctctgg gtgtcggtaa agatcttggc gctttgggcg
      181 caatttcttt cgatgtgaca catgctcgtg cgaattttag ccatgatgat acagaaacgg
      241 gtcagtcata tcgctttctc tattcaaaac gatttgacga cacagacact agcttgcgcc
      301 tggttggcta tcgttactcc accgagggct actataccct caatgagtgg gcatcgcggc
      361 gcaacagccc tgaagacttt tgggaaacag gtaaccgacg tagtcgcgtg gagggaacgc
      421 taacgcagtc gttggggaga gattatggca atttatacct gacattaagc cggcaacaat
      481 actggcatac cgatgatgtc gaacgattaa tgcaatttgg ctacagcagt agctggaagc
      541 gtctctcgtg gaacgtctcc tggagttatt ccaatactgc cagacagggg acggggaaca
      601 accatgccag tgataacacc agtgagcaga tctacatgct ctctttatct gttcctttat
      661 cgggctggtg gggtaatagt tacgccacct attctgtttc gcaaaacgat aattccggta
      721 gctcacatca agcggtacgg cgctggaaag aaataacctt tcatggaatt taatgcagtc
      781 ctataacagt catgatgatg aggttggcgg taatatgtcc ctgacctatg atggctctta
      841 tggcacggtg aacggcagct ataactacag ccaaaattcc cagaggctga attatggtat
      901 cagaggggga attctggcac acagcgaagg ggtaacgtta agtcaggagt taggtgaaac
      961 tattgctctt gttaaagcac ctggggccgc cgggttagaa atagataata tgcgcggtgc
     1021 tgcgacggac tggcgcggct atacggtcaa gacacagcta aacccttatg atgaaaatcg
     1081 ggtagcaatc agcgataact atttctcgaa gtcgaatata gaacttgata ataccgtcgt
     1141 tacgatggtt cccacgcgtg gtgcagtggt taaagcggag tttgtgactc atgtgggtta
     1201 tcgcgttctc ttcagagtgt taaatgcaaa tggtaaaccg gtaccttttg gagccattgc
     1261 tgcgatacaa gatgcaagtt tggcagattc aggaattgtc ggtgaccgtg gcgaacttta
     1321 tctttctggt ctaccagaaa aaggacaggt tacgttatcc tggggagaaa acgcctcaac
     1381 aaaatgcatc ttcaattatt cactttcgac accagaaagt gagagcggat taattgaaca
     1441 gggtgtgaca tgtcattaaa aggattttta tgaacaagta tatcaaacag tggtgctttg
     1501 ctgtgtttat gctctcgtta agtagtgtag cacttgcagc tcctaaaggt atctgtaccc
     1561 ctgataatgg cgtatttcac agtacgcttg atttctcggg atatcttatt accgcgaacg
     1621 aaaataaggt tggaacgaca tttaacacca ctgtaactaa tggatcctca tatcctggac
     1681 gctgtcattg tgatactggt aacgtaggag aattccccta tatctattat acgtcaaaaa
     1741 taaaccaggc attaacttat gctggggttc actctaatat taattattat gatcttaatc
     1801 cgaaccttga tgttgggatt gctatagata ttcttggggt tgggtatgtg aatgcacctt
     1861 ttgaatatca tgctaacaac ccgtctggta atacaaagta caattgcaac cgcattgaac
     1921 ctttaagtat atctagtggg gctaaagcga tagtatattt ttatatcaag aagacatttg
     1981 caggaaaatt gattattcct gaaacaaaga tagtgacatt gtatggaaca attagccgtg
     2041 acaccccggt ggattactca cagccgatgg ctgatgttta tattcggggc gatattacag
     2101 ctccgcaaag ttgcgaaata aataatttac agccagttta ttttgatttt aaagaaatac
     2161 cagctgcaga tttttcatct gttgttggaa gtgcggtaat aacacataaa attactaaaa
     2221 cggtcactat tgagtgtgaa aacctgggga tactgaatac cgatgatatc agtacgtctt
     2281 tttatgctac cgaacccaat acggacaatt caatggtcgt aacttcaaac tctaacgtgg
     2341 ggataaaaat ttacgacaaa aataataagg aaatcaaagt gaacggtggt gagttgccaa
     2401 cagacatggg taaatcaaca gtgtatggtg aaaaatcagg tagtgtaact ttttcagctg
     2461 ctcctgcgag ccttactgga gctcgcccag ccccggggca atttactgca acagcgacga
     2521 taactgttga gattgtgcgc taataggtct gacaacgatt gttcatcaca tcgttgtcat
     2581 ctcacccatt tctttatcac ttttaaatgc atcatgacaa aaagatgaca tgtgggattt
     2641 tcatcttcac atttaaaatc agtaagttat ttggtaattc ctttctgtca taaaaatgtc
     2701 atattcctta catataactg tcacctgttt gtcctatttt gcttctcgta gccaacaaac
     2761 aatgctttat gaatcctccc aggagacatt atgaaagtta tgcgtaccac cgtcgcaact
     2821 gttgtcgccg cgaccttatc gatgagcgct ttctctgtgt ttgcagaagc aagcctgaca
     2881 ggtgcaggtg caaccttccc tgcgccggtg tatgccaaat gggctgacac ttaccagaaa
     2941 gaaaccggta ataaagttaa ctaccagggt atcggttctt ccggtggcgt aaaacagatt
     3001 atcgctaata ccgttgattt tggtgcctct gacgcgccgc tgtctgacga aaaactggct
     3061 caggaaggtc tgttccagtt cccgaccgtg attggcggcg tggtgctggc ggttaacatt
     3121 ccagggctga agtctggcga actggtgctg gatggtaaaa ccctcggcga catctacctg
     3181 ggcaaaatca agaagtggga tgatgaagcc atcgccaaac tgaatccggg tctgaaactg
     3241 ccttcacaaa acattgctgt agtacgccgc gcagatggct ccgggacttc cttcgtcttc
     3301 accagctacc tggcgaaagt gaacgaagag tggaaaaaca acgttggtac tggctctacc
     3361 gtaaaatggc cgatcggtct gggcggtaaa ggtaacgacg gtatcgccgc gttcgttcag
     3421 cgtctgccgg gtgcaattgg ttatgttgaa tatgcttacg cgaagcagaa caacctggcg
     3481 tacaccaaac tgatctccgc tgatggtaaa ccggttagtc cgaccgaaga aaacttcgct
     3541 aatgcagcaa aaggtgcaga ctggagcaaa accttcgctc aggatctgac caaccagaaa
     3601 ggcgaagatg catggcctat tacctctacc acgttcattc tgatccacaa agatcagaag
     3661 aaaccagaac aaggcacaga agtgctaaaa ttcttcgact gggcgtacaa aaccggggct
     3721 aaacaggcga acgacctgga ttacgccagc ctgccggata gtgtagttga acaggttcgc
     3781 gctgcgtgga agaccaatat taaagacagt agcggtaagc cgctgtacta ataaaactcc
     3841 aggccgggta cggtgtttta cgccgcatcc ggcattacaa aatgactttg taaacgcgtt
     3901 taactgaaga gtaacttatg gctgcaacca agcctgcttt taacccaccg ggtaaaaagg
     3961 gcgacataat tttcagcgtg ctggtaaaac tggcggcgct gattgtgcta ttgatgttgg
     4021 gtggcattat tgtctctctg atcatctcct cctggccgag cattcagaaa tttggtctgg
     4081 ctttcctatg gaccaaagag tgggatgcac cgaacgatat ctacggggcg ctggtgccga
     4141 tctacggtac gttggtgact tcgtttatcg cgctgctgat cgccgtcccg gtgagtttcg
     4201 gtatcgccct gttcctgact gagcttgcgc ctggctggct gaaacgcccg ctgggtatcg
     4261 ccattgagct gctggcagcc attccaagta tcgtttacgg catgtggggc ctgtttatct
     4321 ttgcgccgct gttcgccgtt tactttcagg agccggtcgg caatatcatg tcgaatatcc
     4381 cgattgttgg cgcgctgttc tctggccccg catttggtat cggtatcctc gcggcaggcg
     4441 tgatcctcgc catcatgatt attccgtaca ttgcggcggt aatgcgtgat gtgttcgaac
     4501 aaaccccggt gatgatgaaa gagtcggcct acggtattgg ctgcaccacc tgggaagtta
     4561 tctggcgtat cgttcttccg ttcaccaaaa atggtgttat cggcggcatc atgctggggc
     4621 tgggccgcgc gctcggtgaa accatggcgg tgacctttat catcggtaac acctaccagc
     4681 tcgacagcgc ctcgctgtac atgccgggca atagtatcac ctctgcgttg gcgaacgaat
     4741 ttgcggaagc ggaatccggt ctgcacgttg ccgcactgat ggaactgggc ctgatcctgt
     4801 ttgtgattac cttcatcgtc ctcgccgcat cgaagtttat gattatgcgc ctggctaaga
     4861 atgagggggc acgctaatgg ctatggttga aatccaaacc actgcggcgc tggctgaatc
     4921 tcgccgcaaa atgcaggcgc gtcgccgcct caaaaaccgt attgcgctga cgctctcgat
     4981 ggcgacgatg gccttcggcc tgttctggct gatctggatt ttaatgtcca ccatcactcg
     5041 cggtatcgac ggtatgtcgc tggcgctgtt cactgaaatg acgccgccgc ccaatacgga
     5101 aggtggtggt ctggcgaacg ccctggcggg tagcgggctg ttaattttgt gggccacggt
     5161 attcggtacg ccgctgggca ttatggcggg gatttatctg gcggaatatg gtcgtaaatc
     5221 ctggctggca gaagtgattc gcttcattaa cgacattctg ctctccgcgc cgtcgattgt
     5281 ggttggtctg tttgtttaca ccatcgtggt ggcgcagatg gagcacttct ctggctgggc
     5341 gggcgtgatt gccctggcgc tgttgcaggt gccgattgtt atccgcacca ccgagaacat
     5401 gctgaaactg gtgccggaca gcctgcgtga agcggcttat gcgctgggta caccgaagtg
     5461 gaagatgatc tctgcgatta cgctgaaagc gtcggtgtcc gggatcatga ccggtatcct
     5521 gctggcgatt gcccgtattg ctggtgaaac cgcgccgctg ctgtttaccg cgctctccaa
     5581 ccagttctgg agcacggaca tgatgcagcc gatcgccaac ctgccggtga cgatctttaa
     5641 gtttgcgatg agcccgtttg cggaatggca acaactggcc tgggccgggg tattgattat
     5701 taccctgtgc gtactgctgc tgaacattct agcgcgcgtt gtttttgcga agaataaaca
     5761 cggttaacac tttgccggat gcggcgtgaa cgccttatcc ggcctacggt aagcctgatt
     5821 agcgaagtgc atcaggcacg atgaggaaaa gattgcaatg agtatggttg aaactgcccc
     5881 gagtaaaatt caggttcgta atttgaactt ctactacggc aaattccatg ccctgaaaaa
     5941 catcaacctg gatatcgcta aaaaccaggt tacggcgttt atcgggccgt ccggctgcgg
     6001 taaatcgacg ctgctgcgta ccttcaacaa aatgtttgaa ctgtacccgg agcagcgtgc
     6061 ggaaggtgaa attctgcttg atggcgacaa catcctgacc aactctcagg atatcgcact
     6121 gctgcgtgcg aaagtgggca tggtgttcca gaaaccgacg ccgtttccga tgtccatcta
     6181 cgacaacatc gcttttggcg ttcgtctgtt tgagaagctc tcccgtgccg acatggacga
     6241 gcgcgtgcag tgggcattga ccaaagccgc attgtggaac gaaaccaaag ataaattgca
     6301 ccagagcggt tactctctct ctggtggtca gcaacagcgt ctgtgtattg cgcgtggtat
     6361 cgccattcgc ccggaagtgc tgctgctcga cgaaccgtgt tcggcgctcg accctatctc
     6421 taccgggcgt attgaagagc tgatcaccga actgaagcag gattacaccg tggtgatcgt
     6481 cacccacaac atgcagcagg ctgcgcgttg ttccgaccac acggcgttta tgtacctggg
     6541 cgaattgatt gagttcagca acacggacga tctgttcacc aagccagcga agaaacaaac
     6601 agaagactac atcaccggtc gttacggttg attcaggagt gcgttatgga cagtctcaat
     6661 cttaataaac atatttccgg ccagttcaac gccgaactgg aaagtatccg cacgcaggtg
     6721 atgaccatgg gcggcatggt ggagcagcag ctttctgatg caatcaccgc gatgcataac
     6781 caggacagcg atctggcgaa gcgcgtgatc gaaggcgaca agaacgtcaa catgatggaa
     6841 gtggcgatcg atgaagcctg cgtgcgcatt atcgccaaac gtcagccgac ggcgagcgac
     6901 ctgcgactgg ttatggtgat cagtaaaacc attgccgagc tggagcgtat tggcgacgtg
     6961 gcggacaaaa tctgccgtac tgcgctggag aaattctccc agcagcatca gccgttgctg
     7021 gtaagtctgg agtcgctggg ccgtcatacc atccagatgc tgcacgacgt gctggacgcg
     7081 ttcgcgcgga tggacattga cgaagcggta cgtatttatc gtgaagataa aaaagtcgat
     7141 caggaatacg aaggtattgt tcgtcaactg atgacctaca tgatggaaga ttcgcgtacc
     7201 attccgagcg tacttactgc gctgttctgc gcgcgttcta tcgaacgtat tggcgaccgc
     7261 tgccagaata tttgtgagtt tatcttctac tacgtgaagg ggcaggattt ccgtcatgta
     7321 ggcggtgatg agctggataa actgctggcg ggaaaagata gcgacaaata attcaccaga
     7381 caaatcccaa t
//
LOCUS       DAJTNN010000188         7083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTNN010000188 DAJTNN010000000
VERSION     DAJTNN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.190"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..338
                     /locus_tag="OO150_003581"
                     /pseudo
     CDS             1..338
                     /locus_tag="OO150_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            335..2335
                     /locus_tag="OO150_003582"
     CDS             335..2335
                     /locus_tag="OO150_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR5517592.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSGRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAV
                     GFYRLAHVLVQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            2460..2921
                     /locus_tag="OO150_003583"
     CDS             2460..2921
                     /locus_tag="OO150_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR5517593.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASISATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(2962..3432)
                     /locus_tag="OO150_003584"
     CDS             complement(2962..3432)
                     /locus_tag="OO150_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5517594.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3479..4198)
                     /gene="yehT"
                     /locus_tag="OO150_003585"
     CDS             complement(3479..4198)
                     /gene="yehT"
                     /locus_tag="OO150_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5517595.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(4195..5880)
                     /gene="btsS"
                     /locus_tag="OO150_003586"
     CDS             complement(4195..5880)
                     /gene="btsS"
                     /locus_tag="OO150_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5517596.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLQLPWRDEA"
     gene            6102..6167
                     /locus_tag="OO150_003587"
                     /pseudo
     CDS             6102..6167
                     /locus_tag="OO150_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            6395..6643
                     /locus_tag="OO150_003588"
     CDS             6395..6643
                     /locus_tag="OO150_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5517597.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(6759..7028)
                     /locus_tag="OO150_003589"
     CDS             complement(6759..7028)
                     /locus_tag="OO150_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5517598.1"
                     /translation="MLLQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNCVDTSTAPDIEWPEEPDTM"
BASE COUNT         1812 a         1807 c         1726 g         1738 t
ORIGIN      
        1 agagttatta atgaaagtac agttgggcgg cgggaccaat atcgccagtg ccgtggagta
       61 tggtcggcaa cttattgaac aaccagcgaa aagcgtcatt atcctcgtga gcgattttta
      121 cgaagggggt tcatcatcat tactgacgca tcaggtgaaa aagtgtgtcc agagcggcat
      181 caaagtgcaa agtgctggga ctggcagcgc tcgatagcac agcaacacct tgctatgacc
      241 gcgatacggc ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acaccgggcg
      301 agctggcatc atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt
      361 agaactgaca ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct
      421 taaggaactg gaaaatggca acgtcccgga gatcagccat gagaacggcg ctttaatcgc
      481 caccttcagt gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca
      541 gtgcagctgc ggggccagcg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca
      601 acgactttgt gctaccgccc agcctacggg aaaagaagag gaatgggatc cggcaatctg
      661 gctggaagaa ctggctaccc ttcccgatgc cacccgcaaa cgcgcacagg cgctggtcgc
      721 taaaggcatc accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc
      781 gatgagcgat gtgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat
      841 tgaaggcaca ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc
      901 gcagcaagcg gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc
      961 tgacgatccg tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag
     1021 ccaggcatta tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag
     1081 ccgcgcgcag caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca
     1141 actgcgcgcc agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg
     1201 cttacgtcag cttgcggcat taaacagtcg attaaactgc gcacaagaga tggcccggcg
     1261 cgacagtgtt ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc
     1321 cggagaagca aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga
     1381 tattgagcat tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca
     1441 tctttcgcgt agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct
     1501 gtttagtttt caggctggcg cactggcggg tgggcaaatt gtttcacaag cagcaaaacg
     1561 cagtgccgat ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct
     1621 gtcgccagac gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt
     1681 gcgggaatat ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtggataa
     1741 cttatttatt ctgccggtcg ctgagtgtat ttcactcggt tgggacagcg gccgccagac
     1801 gctggatgcg caggtaatca gcggcgaagg tgaagataat ctgctgacgt tatcccttcc
     1861 ggcatctgcc tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga
     1921 cgaccccgtg agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga
     1981 accacgggtg atgatgacaa aaacccgtgc ctgggcgctg gacgcggaaa ctgcgcctgt
     2041 ggcaccgcta ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat
     2101 gcgctgccag gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag
     2161 cgctattagt caggcagagt tgctggcgaa tgacctcacc gcggtgggtt tttatcggct
     2221 ggcacatgtg ttggtacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa
     2281 taacggtgtt ttgctttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag
     2341 tgctttttac taagagttct actccagttc cggactgctc acgccacggt attaggcata
     2401 tcctatatag cccctggtga gagtcaccag tttcttgatt aaataaaatg gagttttaca
     2461 tgaaggcttt caataagctg ttttccctcg ttgttgcatc tgttctggtt ttctctcttg
     2521 ctggctgcgg tgacaaagaa gaatcgaaga aattcagcgc caatctgaac ggcactgaaa
     2581 ttgccattac ctatgtctac aaaggtgaca aggtgcttaa gcaatcttct gaaaccaaaa
     2641 ttcaatttgc ctccattagt gcaaccacca aagaagatgc tgccaagaca cttgagccgt
     2701 taagcgccaa atacaaaaac atcgcgggtg ttgaagaaaa attaacctat accgatacct
     2761 acgcgcagga aaacgtgact atcgatatgg aaaaagtgga ttttaaagcc ctgcagggta
     2821 tttcaggaat caacgtttct gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg
     2881 aactggtgat gaaagccgct ggttttaaag aagtgaaata atctgtcggc ggccatgttt
     2941 cgtatggccg ccatacccgt cttagctttt cttcacatgc tggcgcgctg ccagtccacg
     3001 cagaaaataa cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat ccggtgcgcg
     3061 catcatcgct gtaatttccg gcatcgaaac gcggaactgc tgttcggtga ggatagccag
     3121 aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt
     3181 aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat
     3241 caggccattg aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc
     3301 ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattac ccagcgccag
     3361 aatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc
     3421 gttacttagc atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt
     3481 acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc
     3541 cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg
     3601 cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt tccagggtac
     3661 gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca
     3721 tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg
     3781 tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc
     3841 tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca
     3901 gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga
     3961 gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga
     4021 tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga
     4081 tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa
     4141 atacacgcag gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc
     4201 ctcgtccctc catggtagtt gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac
     4261 gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc
     4321 cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat
     4381 caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga
     4441 tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg
     4501 ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt
     4561 ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga
     4621 aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg
     4681 ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg
     4741 attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc
     4801 atattgcccg gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg
     4861 gtttattgaa ctgaataaac ggtttttggc ttcatacaat ttgatggtgc ccatcacccg
     4921 ctgattttca ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa
     4981 agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc
     5041 ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc
     5101 agtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg
     5161 atacagcacc tgtgccactt tcatgctgtt cacttcgtta aacccctggc gcaaaatgcc
     5221 ttccgtcgag gcagccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa
     5281 catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac
     5341 catcattggt gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat
     5401 cgccaggatg atcaacattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat
     5461 ggggttaaag actttatcag tacgcccgcg acgaatcagg atgctgtgta ccaggccgcc
     5521 gagcaatcct tcaacgatgg tcgagatcat gcaacttagc gcggtcatgc cccccatcga
     5581 atatcgatgt aacccgccgg tcaggccaac cagcccaccg acgaccggac cgccgagtaa
     5641 gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc
     5701 aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg
     5761 cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc
     5821 aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat
     5881 acccgcaaac cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct
     5941 ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa
     6001 aataaaatca ccctatagat gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc
     6061 gtctaaagtt aaaccgggac ctcgcgagca agggtgagac gatggcgctt tacacaattg
     6121 gtgaagtggc gttgctttgt gatattaacc ctgtcacgtt acgcgcgtga cgcaacattt
     6181 taaaaaatcg acgaagatcg tccaggagcg ccgtttttca agggttggat agatgattcg
     6241 tttaccatgt cgacgaactt cgacgaattt cgccgataaa aagtgtccca tacatgcccc
     6301 attgatgcac cataaacaat atcagaaatt gatactggtt atccatacag ataaaaataa
     6361 cacctgtaca taactacagt tacaggaggg tgttatgcgt gttgagatct gtatcgctaa
     6421 agaaaaaatc actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag aattaacccg
     6481 acgcatcagt aaacgttatg acgatgtaga ggtgatcgta aaagccacca gcaacgatgg
     6541 cctttctgtt acacgcaccg cagataagga ttctgcaaaa acatttgttc aggagactct
     6601 gaaagatacc tgggagtctg ctgacgagtg gtttgttcac taataaacac gtaaaatcgg
     6661 taacggctgg aaatcattca atactcgcac tatcgaaagt ttaccagcca accgcagcac
     6721 gtcctgcata cgtcgtgtct acggtttttc tttttcgctt acattgtgtc tggttcttcc
     6781 ggccactcaa tatcaggtgc agttgatgta tcaacacagt tcagcaacac ccgatacttc
     6841 ttccaggctt ccagcaacga gttttcttcc tccgttgcga tctccagatc tacagcatcc
     6901 tgaagtggcg caatatgctc actgaattcc tggatgtaga actgtgtggt gacggtcttc
     6961 cagccattcg gctcctgctg tatcgaagca taccaggcta tttcaatatc gctatgctgc
     7021 agcagcattt aaccccttgt aattcatcgc cataattgat ttaattcaca aataaaacta
     7081 taa
//
LOCUS       DAJTNN010000189         7026 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTNN010000189 DAJTNN010000000
VERSION     DAJTNN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.191"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..247
                     /locus_tag="OO150_003590"
     CDS             <1..247
                     /locus_tag="OO150_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517599.1"
                     /translation="HARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILN
                     TLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(216..1952)
                     /gene="aceK"
                     /locus_tag="OO150_003591"
     CDS             complement(216..1952)
                     /gene="aceK"
                     /locus_tag="OO150_003591"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR5517600.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASELWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2233..3530)
                     /gene="aceA"
                     /locus_tag="OO150_003592"
                     /pseudo
     CDS             complement(2233..3530)
                     /gene="aceA"
                     /locus_tag="OO150_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(3560..5161)
                     /gene="aceB"
                     /locus_tag="OO150_003593"
     CDS             complement(3560..5161)
                     /gene="aceB"
                     /locus_tag="OO150_003593"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR5517601.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFGDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            complement(5430..6359)
                     /gene="metA"
                     /locus_tag="OO150_003594"
     CDS             complement(5430..6359)
                     /gene="metA"
                     /locus_tag="OO150_003594"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5517602.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6516..6963
                     /locus_tag="OO150_003595"
                     /pseudo
     CDS             6516..6963
                     /locus_tag="OO150_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1684 a         1807 c         1826 g         1709 t
ORIGIN      
        1 ccatgcacgc ctgtttggat ttaccgctga agatattatg gatttttggc aacacaaagc
       61 gccacaaaaa tactctgcct ttgagttggc ttttgaattt ggtcaccggg ttattgctga
      121 attaatcctt aatacattaa ataagatggc tgaaagcttt ggctttacgg ataaccctcg
      181 atacattgcg gagaaaaatt atatggaagc tttactcaaa aaagcatctc cccataccgt
      241 acgctaaatc tttgcctgcg ccgatacgca taaacatctt ccacatgccc ttcacgtatg
      301 cggttttgta gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac
      361 agcggaccaa tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg gaaaacatcg
      421 cccggcgaga cgctgtacca cagttcgctg gcaagttcgt cttccggata gcgcggcggc
      481 gggatgtcgc ggaaattcac ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc
      541 acacgcccgt gacgggtgac accgaagttt ttaaacagca tgtcgccagg aaaaatgtta
      601 gcagcggcaa gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg caactgctga
      661 ccttccactt gttccagcca gatattgagc ggcaccatcc gccgctcgat atagagatgg
      721 cgaattacaa tctgctcacc aagatcggtg attttttccg ctgcttcctg gagcagtaat
      781 tccattaatg ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg
      841 tccgccattc ggcccacacg gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga
      901 gcggcagaca tctctttctg cggcgcgaac ttgtctttga tgactttgaa tacccgatca
      961 aagcccggca gcgtaaacac caacatcacc attccacgaa tacccggcgc ttcaatgaac
     1021 tgctcattac agccctgtag ataaacgaga tattcgcggt agctttcggt tttggcgtgc
     1081 ttctggcagc cgatagccat atacaattca gcagtggttt tacctggcag aatttcccgt
     1141 agccactcga ccagcgctgc gggcagcggt gcataaacca taaaataaga acgcgcaaag
     1201 ccaaaaacaa tgctcgcttc ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg
     1261 tcgtccgtct ggtggatcgg cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg
     1321 cccaccagcc aggcggcttt attgcggtaa aacagttcgt tcgccacctg taaatgactt
     1381 tccgcgaggt tgtctgtccc cagcgtttcc gtcagatggc gaatgatgta atgaatgtca
     1441 cggcttttat tctgccagcg caggcgcagc ggcaggtcgc tgataacgcg catcagtaga
     1501 gattcccagc cgtgatcggg gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag
     1561 cggcgctctg gctgagagct aaagataaaa agccgctcgg gagtaagcga gcggtggtca
     1621 aataaccgac agtacacgga gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc
     1681 ggcaacagcc gcgtgtaatg ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg
     1741 ccgttagtaa tgcagcgcag ttgctccacg accagaccaa cgtgatgatc gtaaagatgg
     1801 atacggtttt tcatcgccag ctggacagca tgccagtcgg cctgttcgaa acgctgctgc
     1861 gccccggaag tcacttcgag gaatcgacca tactgagcgt cgaagccttg caaaatggtt
     1921 tgagcaatca gtaattccag gccacgcggc atttagcgcc ctcatcagga gcagagaatt
     1981 gccggatgcg gcgtaaacgc cttatccagc ctacgttcgg caacggttgt aggcatgata
     2041 agacgcgcaa gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtaaatgcct
     2101 tatccggcct acgttcggca acggctgtag gcatgataag acgcgcaagc gtcgcatcag
     2161 gcatcgtgca ccgattgccg gatgcggcgt gaacgcctta tccggcctac agtcagcaac
     2221 ggttgttgtt gcttagaact gcgattcttc agtggagccg gtcagcgcgg tgactgaaga
     2281 cgtgccgccc tgaataatag tcgtcacttt atcgaagtaa cctgtaccca cttcctgctg
     2341 gtgagatacg aaggtatagc catctttcgc ggcggcaaat tccggctgct gcactttctc
     2401 aacgtagtgc ttcataccct cgccctggtc ataggcgttt gccaggtcaa acatgttgaa
     2461 ccacatgctg tggatacctg ccagggtgat gaactggaac ttgtagccca tatccgacag
     2521 ctgctgctgg aagctggcaa tagttttgtc gtcgaggttt ttctgccagt tgaacgacgg
     2581 cgagcagtta taagccagca gtttgcccgg atatttcgcg tggatagctt gtgcaaagcg
     2641 acgcgccagt tccagatccg gcgtggaggt ttcacaccag accaggtcag catatggcgc
     2701 atacgccagg ccacggctga tcgcttgctc aatgcccgca tgagtacgga agaagccttc
     2761 actggtacgc tcgccggtaa taaattcgct gtcatacggg tcgcaatcgg aggtgatcag
     2821 atccgcggca tcggtacgag caaccagtag ggttggaacg cccgtcacgt cagctgccag
     2881 acgcgccgcg accagtttct gaatagcttc ttgagttggc actaaaactt tgccgcccat
     2941 gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg ctgcaccggc
     3001 ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac cggcttccgc
     3061 atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct caatgcccgc
     3121 ggaccattgg atctgatccg cacggcggaa agtattgttg atccgctcca ccacagctgg
     3181 caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca ggttagcgtc
     3241 cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg cctgttgcag
     3301 cgcctgaccg ccagtcagtg caccgaggct gttgatgtag cttttttcga ctcgccatgc
     3361 agcagacgcc acattctcgc cgcgcccagt tgcgccaggg tgcattcagg attgactgaa
     3421 tcgcgtaatt tcaccacatc ttccgcgctg tatgggcgag taatgccttc ccaacgcggt
     3481 tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc
     3541 tccatagtta tgtggtggtt tacgctaaca ggcagtagcc tggcagggtc aggaaatcaa
     3601 ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc ggcatcgcca aaacgcccct
     3661 gggagaaacg ttcttcgccc agttcgctgg caatgacttt catctcttcg cccagcatct
     3721 ggcggaacaa ggctttagtt accggtttgc cattgctcaa cattttttga tgatggatcc
     3781 actgccagat tgaggtacgg gaaatttcag ccgtcgccgc atcttccatc aggccataaa
     3841 tcggcacaca gccgttgcca gagatccacg cttcgatgta ctgcacggct acgcgaatat
     3901 ttgcgcgcat accttcttcg gtgcgttcac cgtcacaagg tgccagtagc tgatcggcag
     3961 taatcggcgc gtcttgttcg cgcatcactt caagctgatt tttacgggag ccgagaatgt
     4021 cgttgaatac cgccattgcc gtatccgcaa ggcctgggtg agcgatccat gtgccatcgt
     4081 gaccgttatt ggcttccagc gatttatccg cttttacttt gttgagcacc tggttattgt
     4141 gctcttcatc tttgctcgga ataaacgccg ccatgccgcc catcgcaaaa gcaccgcgtt
     4201 tatggcaggt tttaatcaac aggcgtgagt aagcattcag gaatggttta tccatcgtca
     4261 ctgcctgtct gtctggcagg acgcgatcgg gatagttttt caacgttttg atatagctga
     4321 agatgtaatc ccaacgaccg cagttcagac caacaatatg gtcacgcagc gcgtgaagga
     4381 tttcatccat ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc
     4441 cgcgcggcag attaaagcga tcttctgcat agctgaagac ttcgctccac caggccgctt
     4501 cctgccagga ctgggttttc ggcagataga aatagggacc actgcccttt gccaacagag
     4561 cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag gctgccgggg attgcctcac
     4621 cacgccaggt gacatgtttt tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg
     4681 ctggattggg cttgagctgg taaattttgc ctgcttcatt ggtatagctg atggtaccgt
     4741 taactgcatc acgcaggtta atttgcccgt cgatcacttt gttccagtcc ggggccagtg
     4801 aatcttcgaa atcggccata aagactttca cattggcgtt gagcgcgttg atcaccatct
     4861 tgcgctctac cgggccagtt atctctacgc ggcggtcttc taagtccgca ggaatcccgc
     4921 gaattttcca atcagcatcg caaatggaag ctgtttccga aataaaatca ggcaacgttc
     4981 cgttatcaat atcttgctgc tgctgaatgc gcgctgccag aagtttattt cgttgtggcg
     5041 taaaatgcgt caccagctca gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct
     5101 cctgctcgcc atacggcctt gtgaaagcca gttcatcggt tgttgttgcc tgttcagtca
     5161 tcgtgcagct cctcgtcatg gatccgaaaa cttccccact gtgaacgaag gatcgttgtg
     5221 cgcttttcgt tcagcacaac taagactact catttaaaat gcaaaatcaa aaacaatttc
     5281 cattttaatt gaaaatgctt gataacttat tgataacaat gtgaataaaa tttattcacc
     5341 tcctgaggtg cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt aaggtgctga
     5401 atcgcttaac gatcgactat cacagaagat taatccagcg ttggattcat gtgccgtaga
     5461 tcgtatggcg tgatctgata gacgtaatag ttgagccagt tggtaaacag caaattaccg
     5521 tgactacgcc agctcgctcg cggtgtattt tgcggatcat tgtgcgggaa atagttatac
     5581 ggtacttccg ggtctagtcc ggcttccaca tcgcggaaat attcctgcgc cagcgtttgc
     5641 gcatcatatt cgggatggcc cgtcacaaag gcaatgcgct tatctttgct ggcaaacaga
     5701 tatgcatccc cttcttccgt ctctgccaga atttccagat cggtgtaatc acgaatcaac
     5761 gctgccggaa agtcagcata gcgcgaatgt ggtgccagga atgaatcatc aaagccacgc
     5821 gtcagaagcg catgaggatg gagaatatga tgctcgtaaa cgccagagag tttgtcggtg
     5881 cgagtttgct taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc ccagcagaca
     5941 aacagcgtcg aggtgacgtg atctttcgac cactccagca cctgtttgat ctgcggccag
     6001 taagcgacat cattaaactc caccaggccc agcggcgcac cggttacaat cagaccgtca
     6061 aagttctgtt cctgaatatc ttcaaagtta cagtagaagt tgttcagatg ctctgcgggc
     6121 gtgttgcgcg attcacggga atcgatgcgc aacagctgaa tatcgacctg caaaggtgag
     6181 tttgaaagca ggcgcagaaa ctgattttca gtttcaatct tcttcggcat caggttaagg
     6241 atcagaacct taagtggacg aatttcctga ccagacgcac gagaagttgt catcacaaag
     6301 acgttttctt cacgcaagaa attgacggcg ggtagctcgt ccggcacacg aatcggcata
     6361 acctgattac ctcactacat acacttatac gtttagacat ccagatagct gaagataacc
     6421 agaaaatttg ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa ggaaaagact
     6481 acgttagaat ataagaatga ctaaaaggag agaaaatggt tattagtatt cgccgctcac
     6541 ggcatgagga aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat gccacacacg
     6601 attttttatc agcagagtat cgggccgagc tggaggtgct agctagttcg ttccttcctg
     6661 ccggaagcgc cgttgtggat cgcggttaat gaacgggatc agccggttgg gtttatgttg
     6721 ttaagtgggc agcatatgga tgcgctgttt atcgatcctg atgtgtgcgg ctgcggcgtg
     6781 ggtcggatgc tggtggagca tgcgctctcg atggcaccgg aactgacaac caacgttaat
     6841 gagcaaaatg agcaggcggt tgggttctat aagaaggtgg gttttaaggt tacgggacgc
     6901 tctgaggtgg acgatttggg gaaaccgtat ccgttgctga atctggcgta cgtgggggaa
     6961 taagccgccc ctgccgtgcc tgcccaggct ggagaagacg cgccagcgtc gcacccggca
     7021 tttttt
//
LOCUS       DAJTNN010000190         6991 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTNN010000190 DAJTNN010000000
VERSION     DAJTNN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.192"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..561)
                     /locus_tag="OO150_003596"
                     /pseudo
     CDS             complement(1..561)
                     /locus_tag="OO150_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            719..1903
                     /locus_tag="OO150_003597"
     CDS             719..1903
                     /locus_tag="OO150_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCR5517603.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            1903..2413
                     /locus_tag="OO150_003598"
                     /pseudo
     CDS             1903..2413
                     /locus_tag="OO150_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            2469..2843
                     /locus_tag="OO150_003599"
     CDS             2469..2843
                     /locus_tag="OO150_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5517604.1"
                     /translation="MKDIDTETRNNTVADNVTAVEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            2852..2956
                     /locus_tag="OO150_003600"
     CDS             2852..2956
                     /locus_tag="OO150_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR5517605.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVS"
     gene            2994..5801
                     /locus_tag="OO150_003601"
     CDS             2994..5801
                     /locus_tag="OO150_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5517606.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVAFGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGCFYDKD"
     gene            5814..6302
                     /locus_tag="OO150_003602"
     CDS             5814..6302
                     /locus_tag="OO150_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5517607.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(6331..6929)
                     /locus_tag="OO150_003603"
                     /pseudo
     CDS             complement(6331..6929)
                     /locus_tag="OO150_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1586 a         1753 c         2033 g         1619 t
ORIGIN      
        1 attgccgctt tttaccgtgg cgattgctcc cagcatttcc gccatgcgcg taagaaagga
       61 catgtcgctt tcttcggtct ggtcggcgtg gtcgatttcg atatccatca gcatttcgct
      121 gatttgcggt ttcagaccgt accgatgagt gatggcggat accacacgct caacggtcac
      181 atcatgccag gacacctcac gtttaacgtt aaattcatcc cgaaaatctg cgcttctggc
      241 tgaaacagtc agcctgtccg gcggtccttc gtgagcgatt tcatcaacaa tgtaagtgcc
      301 tttttctgtc agcggttccc ctttccagcc aatgagaacc gtcagacgcg cgccccgtgg
      361 cggtagctgc aactgaccat ccgcatcatc cagcgtgatg gtgagctggt ccgcctcaaa
      421 tccccggttg tcggtcagtt acaggctcat caggcgctcc gccacgcctg acagcgtttt
      481 accctccgag agaatatcaa aatccggcat tttcacgggg gccgtgccct gactgagcaa
      541 ttgcatggtg gtgtcggtca tctgctccct ccctgtgcgg catggtcgca tgtgcgtgcg
      601 gagggggtta ctgctttttg ttgtcgccgt ggcgggagaa cggcgcaggg gtgagattac
      661 gcgcgtggtg ggtgatgatt gttgctgaat catttaacgg atacaagggg ctgaagctat
      721 gagtgaaact cgttttcatg gtgcccgtgt tacggagaat accgacctgg taacagcgat
      781 taacgatgtt gattccagcg ttatcggtat cgtggcaacg gcggatgatg cggacgcgaa
      841 gctgttcccg ctgaacaagc ccacactgct gacccgcgtc aatgacgtgc tgggaaaatg
      901 cgggacaacg gggacgcttt atcgtgcgct taaggccatc gcagaccagg tgagcacaaa
      961 ggtgatcgtc gttcgcgtgg ctgaacacaa agaagaagac ggaaagacgc aggatcaact
     1021 ggttatcggt ggttctgagg atgacggcag ctatacgggg atgtatgcgc tgcttgttgc
     1081 agagcaggat gaaagcatcg gataccgtcc gcgtattctg gccgcgccgg agctggacac
     1141 ggaggcggta acaaaatccc tgtgcgtgat tgcagataaa ctgcgcgcgt ttgtgtatgc
     1201 ctcatgtcat ggctgtaaca cgatggccga ggcaattacc taccgccaga aattcaacga
     1261 acgtgaggtg atgctcttat ggccggactt catcgcctac aacccgaaaa atggcaaaaa
     1321 cgaaacgttc cccgcgcctg cctatgcgtg cggccttcgt gcgtacattg accatgaaca
     1381 gggatggcac aaatcgctgt ccaacgttcc ggttaaaaat gtgctgggga tgtcgaggca
     1441 tgtgttctgg tcgttgcagg ccgaagacag cgatgccaac agcctcaaca acaaagaaat
     1501 cacgaccatt attcgtcgca acgggttccg cttctggggt aaccgcacac cggaaacgaa
     1561 cgcctacatc tttgaggtgt atacccgaac cgcacaggtg ctggctgatt caattgcgga
     1621 agcgcagttt gaaaccatcg acagtccact gacgcctgcg aatgtgaagg atgttatcag
     1681 tgccatcagg gcaaaactgg attcactggt gactgccggg aaactgattg gcgcggagtg
     1741 ctggtatgac gtggtggata acagcaccac ggatttacgt cagggacgtg tgcgtattcg
     1801 ctacaaatat acgcccgttc cgccactgga agacatggag ctttaccaga cgtttactga
     1861 tgaatacttt gaacccgcat ttgcggtgct gggaggtgcc tgatggctgt gccaaaacat
     1921 cttcgctttt ttacgctgtt tgtggatggt gaaaacgaag tgggtaatgt gacgtccgtc
     1981 actctgccta agctgacgcg caaaaccgac agctaccggg gtggtggcat gatgggtgcg
     2041 gtaagtattg atctcggtct ggacgactcc gcgcttgatg cgagctttgt catgggggcg
     2101 cagttcgtga gctgttcctt aagtatggcg gcacgattga cggcacgctg ctgcgttttg
     2161 cgggtgaata ctacaccgat gcagaaagcg acctgtatga agtcgaaatg cgcggacgtg
     2221 tgacggaaat tgatatgggg gaagccaaac agggcgaagc cacatcacac acttacgcca
     2281 ttaaaacacc tactacaagc tgagtgttaa cgatcgcccg ttgtgggaga ttgacctgct
     2341 gaacttcatt taccggaagg acggcaagga cattgtgccc gatcgcatcc gttccgcgct
     2401 tgggcttggc tgataagtaa tatgctggcg gcgcagtgcg tcgcctctga ctgaaaagga
     2461 gacaacagat gaaagacatc gatactgaaa cccggaataa caccgtggcg gataatgtga
     2521 cggcagttga ggatatggct gtcgaacgtg gcgtaaaact gacccgacca attgagcgtg
     2581 gtggcgaaaa aatcacgtat gtggagatca ccggggctat tgagcaggct ggatccctgc
     2641 gtggtctgtc gctgtctgat gtgctgaatc tgaaagcgga taccatgttc acgctgttgc
     2701 ctcgcgtgac ctcgccacga ctggatgaag tgatgattaa aaaaatgtcg tcacgcgatt
     2761 ttattcagtt gtgcgctgtg gctgtaaatt ttatgagcga gccagactct ggcgcgaaga
     2821 gcgtgcagga gacggcagcg taatcaccct ggtgtgcttt gagcacatcg aagatctggt
     2881 ggcggatatt gccgccattt ttaactggtc gcccgccgaa atcttcatga tgacgcccgg
     2941 cgaagtggtt agctgacgtg agcgggcggc acttcgtagc gggaatgcag acaatgaaga
     3001 ctcttgatat ccgggtcgct ttcagcgccg ttgacaggct gacccggcct gccgaaaacg
     3061 cccgccgcct gatggggcag tttggtgact ccatccagcg aacgcagggg gcgatcaaaa
     3121 atctcgagcg tcaggcgcga tcatttgagc gcgcccgtga cgctgtcagt aaagcggatg
     3181 ctggcatcgt gaaagcacga cgccagctta acgcccttaa tcagttacaa cgcacgggta
     3241 cagtgctcag cgaaaaacaa caaaagctga tgcagcagtt aagcacccgg cttgaacgcc
     3301 tgaatgaatc gcgcacacgg gaaattcaga aaatgcggga gcttggcgga gagctgaaac
     3361 gccacggcat ttccctgaca ggcagcgata acaccatcca gcaggccatc agacgcaccg
     3421 aacagtacaa caaccagctt gaacgcgaac ggcaggcgct tgcgcgtgta acacgggcgc
     3481 gtgagcggta ttcgcgcgcg caggaaacag cgggaaaact gaaaacaggt ggtgcgctgg
     3541 caattggtgc ggcagcggcg ggcggctatg ctgccgggcg ttttttgcag cctgcgatcg
     3601 ggttcggcaa agagatgtcc cgcgttcagg cactgacgcg aatcgaccag aacagcccgc
     3661 agtttaaggc gctgcgtgag caggcgttaa aacttggctc tgaaacgcag ttcaccgcag
     3721 gcgatgccgc cagtggacag gcatttcttg caatggctgg ctttacacca caggccattc
     3781 aggctgcgct ccctggcgta ctgagcatgg caacggctgg cggtatggac ctcggtgaga
     3841 cggcggatat tggctcaaat attctgacgc agttcggtct ttctgctgac cagatggacc
     3901 gggtcggcga cacactcacc gcagcgttta cccgtaccaa cactgacctt cgcgcactgg
     3961 gtgaaaccat gaaatatgca ggtccggtgg cgggtaagct gggaatatct ctggagcagg
     4021 ccgcagcgat ggcgggcgtg ctggcgaata tgggtatcag ggggagtgat gctggtacgg
     4081 caatgcgtgc cagcctggct cgtctggcat caccgccaaa ggcggcagca gaggcgctga
     4141 aagagcttgg cgtgtctgtc tcggatgccg ggggcaaaat gcgcccgatg gaggatgtgc
     4201 tggctgacct ttataaagct acccgcaaat acggggaagt tgaccgggta tcgttcttta
     4261 aggacattgc cggagaagag gctttcacat catttatggc cctcgttgat gcggcaggtg
     4321 acggctcctt acccaaactg agaaaagaac ttgaaggcgc gcgcggtgag gctgaacgca
     4381 cggcaaaggt tatggccaac aaccttgacg gcgatctgaa atcactcagc agtgcatggg
     4441 aagggttgcg catccgcatt gcagatctga ttgacggtcc gctgcgttct gtcacgcagt
     4501 ggctcacgcg tgtggtatca aaggtgacgg cgctggcgca ggcccatccc gcactgacgc
     4561 gccagctact gattgcaggc ggtgcattgc tggcaatgac tgcaacggtt ggctcgttgt
     4621 cgctggctat tggtgtgctt gctggcccgc tggcaaaact gcgtcttggt ttttccctcc
     4681 tgaccggatc aatgaatgct gtcagggttc tgccagcact atggggaatg gtgacgggtt
     4741 ccgtttcttt actgggaggc gctatcgggg cgttgttcag tccggttggt cttatcgtgg
     4801 ctgcgcttgc cggagctgcc gttcttatct ggaaatactg ggatcccatc agggcatttt
     4861 ttgccggggt gttcagcggg attatggaaa ggctgacccc gttgcgcgaa acctttgaac
     4921 ggtttggtcc tgtttttgac gcaatcggga gtgggatcag ccaggtgttt aactggttta
     4981 aatcgctgct gtcaccgatg gagtccagca aggaaactct ggataaatgt accagtgctg
     5041 gcgagatatt cggtaacgtt cttggcggtg cgttacaact tgttctgaca cctgcaaaaa
     5101 tgttgctgga tacgctggcg tggatacttg aaaagcttgg tgtgcttccg gatgaagcgg
     5161 aaagggcgcg caagaaaatc gaagacgcac agcgtgcggc cattcttcag gacaaggttg
     5221 ccttgcttca gggggatctg gcgaaaatca atccgccgaa gcctgtggaa aatggcaatg
     5281 gcaccggagg tgataacccc aaagacaata aaccgctcac agacagcaat accggtacgc
     5341 tgcgcagact cagcaaaatt gctgataaca caggtaagct ggttgaggag acgaaaaaac
     5401 gcattggccc cggcgatatt gtctttaaaa acctgccccg agcacttgct gttcgtgggg
     5461 agtggcagga gcggaagatt gcgcaggtca gtaagcctgc ccccgcaatt aatatcacac
     5521 ccgtggttcc ggctccgctg cctccggcgc tggtccctgt tgttgcggcc agctcccgcc
     5581 cggtggcgga ggctatacga tctccagtgg catcagttcc tgtaacttcc cgtaaccggg
     5641 agcctgttgc cttcggattt ggtggtgaaa ttcatgttca tctgcataac gttgttacgc
     5701 agaatccccg cgaactggcg aaactggtcg gtgaaatggt cagggcagaa atggaacggc
     5761 gcgcccgtgc cgggcgtggc tgtttttacg ataaagattg aggagtcatg gccatgatga
     5821 tgatctacgg catgtttgtt tttgagctgc gcacgctgcc gcatcagcag ttacagcaaa
     5881 acaaaagctg gcggcatgtg aaaaatgaac gcgttaaccg ttcagcaagc tggcagtata
     5941 tcggtgcagg tgatgatcgc atcgttcttt ctggtgtgct ttatcctgaa attacaggtg
     6001 gcgaagtgtc gctgtcgctg ctgactacgc aggcgtatac aggacgaccc tggcctttga
     6061 ttgatggcgt cgggcagatt tacggcatgt atgtcctgac tgaaacgaat acgacccgct
     6121 ccgagtttga tcgctacggt aaggcgaaaa agatagaatt ttcactgact cttgaacgct
     6181 gtgatgagga tttgcgggag cgcctgcaat cctcatcgtt cagtgatatg ctgtccggct
     6241 tcaaagataa ggtgacatca tctcttaaca gcgcggccag ttcagttaaa gggctgttct
     6301 gatttaacac aaaaaccgct aatggtcaga ttagcggttt tcattttcct gagtctgccc
     6361 ggttgtttct tcagtctgta tatcgcctac agggtgataa cgataaatcg tcgatgtgcc
     6421 aatgccgtaa attattgcca gttgttttct gtcgtgcccg tttttgatta gccttgctat
     6481 ttgctcatgc tgttcttttg tcagcttcgg tcgacgtccg cctgtgcgcc cccttgcgcg
     6541 cgctgccgcc agcccggcca gtgtacgttc aacaattaat tcacgttcca tttcagccag
     6601 ggcacccatc acgtggaaga aaaaacgccc cattggagaa gatgtgtcta tgctgtcggt
     6661 cagactgcga aaattaatcc ctcgctcccg tagttccccg acgagagaaa tcagatgttt
     6721 catgcttcgc ccgaggcgat ccagtttcca gacaaccagc gtgtcacctt ttgaaggcgc
     6781 tttaaagcgc gttttaatcc cggtcggtct gtctttgtcc cgcttaattt atcttcaaat
     6841 atttgttcac atcctgcaca aacaagagcg tttcgttgca ggtctgtatt ctggtcattt
     6901 gttgataccc ttacatagcc aatcagcaca ctgaatctcc cgtccaaaag cgcaaatcat
     6961 gccatgcagg ccagaaacgg ccattattta a
//
LOCUS       DAJTNN010000191         6939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTNN010000191 DAJTNN010000000
VERSION     DAJTNN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.193"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..287
                     /locus_tag="OO150_003604"
     CDS             <1..287
                     /locus_tag="OO150_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5517608.1"
                     /translation="FLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(438..3761)
                     /gene="mscM"
                     /locus_tag="OO150_003605"
     CDS             complement(438..3761)
                     /gene="mscM"
                     /locus_tag="OO150_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5517609.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(3783..4751)
                     /gene="psd"
                     /locus_tag="OO150_003606"
     CDS             complement(3783..4751)
                     /gene="psd"
                     /locus_tag="OO150_003606"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5517610.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQSVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(4848..5900)
                     /gene="rsgA"
                     /locus_tag="OO150_003607"
     CDS             complement(4848..5900)
                     /gene="rsgA"
                     /locus_tag="OO150_003607"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5517611.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            5995..6540
                     /gene="orn"
                     /locus_tag="OO150_003608"
     CDS             5995..6540
                     /gene="orn"
                     /locus_tag="OO150_003608"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5517612.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDSFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            6751..6826
                     /locus_tag="OO150_003609"
     tRNA            6751..6826
                     /locus_tag="OO150_003609"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6784..6786,aa:Gly,seq:gcc)
     gene            6863..6938
                     /locus_tag="OO150_003610"
     tRNA            6863..6938
                     /locus_tag="OO150_003610"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6896..6898,aa:Gly,seq:gcc)
BASE COUNT         1605 a         1865 c         1869 g         1600 t
ORIGIN      
        1 tgttcctcgc gctggctttc ccattcttta aagcacgtca ggatctcgac agaccatttg
       61 tgattttcaa aacgcgtatg tcggcaatga ttgcgacggt ggttgtcgta ctggtggtga
      121 catttgcgaa cgtcttcacc attattccgc ctgtggttga agccggagac tgggacagca
      181 cattgtggat gattggcggc cctgtcttct tctcgctgtt agcgatggcg atttaccaga
      241 actattgcag tcgcatggcg aataaacctg agttagctct cgactgatca tatgaaatcc
      301 tgcaactcat cgtctctccg gcgatgagtt gtcaggaagt aatgcgtatg gatgatactc
      361 tccagggccg gtgataactg aagatgatgt taagctgcta aatgagccac gttgacatca
      421 tttggcagct tcatgcatta caaacttccc gcctgacgac ctttgcccgc agacgtcagc
      481 gttctccccg tttgtttacc gttgaggctt tccagacgca tctggaaggg cgggaatggc
      541 atatcgatac cgtgggcatg gaagccagcc agaatcagct ggtggatctc atggcgtagc
      601 ggcatacggt gacccatctc agcggcataa atacgcagct cgaaaatctg aatcccttgt
      661 tgcagatcca ccaggaagac ttccggtgcc gggttgtcga tcaccagcga gcagcgacgc
      721 gctgcggtga gcaggatttc cgtcacttct tcactgttgg catcagcagg ggccggaatc
      781 gtcaacacta cgcgcgtaac cgagtcagag agcgaccagt tgataaactg ctcggtaata
      841 aacgccttgt tcggcacgat tatctcttta cggtcccagt cgctgatggt ggtggcgcgg
      901 gtgttaattt tcgtcacgct accggtgaga tcgcgaattg tcaccgtatc gccaatgcga
      961 atcggttttt cgaacaggat aatcagacca gagataaagt tggcgaaaat ttcctgcaaa
     1021 ccaaaaccga gaccaacacc gagcgcggcg accagccact gcaatttcga ccattcaata
     1081 ccaatcatcg agaagccgac cagcccgcca atcagcatca gcagatattt ggtgatggtg
     1141 gtgatggcgt agcctgtacc cggcgttaaa tccaggtgct gcaaaatcgc cagttccagc
     1201 agcgcgggca ggttgcgcac cagctgcgtg gtgatgataa acaccagaat ggcaatcagc
     1261 accgcaccga gggtaattgg ctccagactt tctacgcctt gtaccgtgga ggtgacatcc
     1321 cacagcgaaa tattttcgag gaagccgaaa gcggaatgga tttctgacca cagcacaatg
     1381 acagaaagca gggcgatcaa catcaaaatt gagcgcacca gccgcaagga ttgcgcactg
     1441 atggcatcga gatcgacttc gctttcatcg acttcaattg ctccttccgg gctactgtga
     1501 tgatgcgctt cctcttcgcc acgcgcacgt tgcgctaaca tctctgcccg gcgatgcttc
     1561 gcccggtcaa acgccagcct gcggcgctgg atcagcatcc agcggcggat aacgtgataa
     1621 accaccagta gcaggaacca gatggcaacc gaggtttcaa gcctcgctaa cagcgcctgt
     1681 gccgttgcca gataacccac agccgacgcc agaatggcaa ccaacggcgc gccaatcatc
     1741 atgttccaca gcatatggtt ggtaatgttg tcgccgctgc cctctttgtt gagatacagt
     1801 gggatcccgg cctttttcag gctgagggtg accaccgcca gcgcaccgca aatgaggata
     1861 aagcaaagcc gtcccagcga accggagaat tcacggtcgt cgaggttatc gaacatcatc
     1921 agcgccataa tcagcggcac aataagcccg atgctcatca ggtagtagcg catcccacgg
     1981 gaaacacgtt cgcgcggcca gccaaaatga gcgataaaca agccgttcgg gcgggcaaag
     2041 gtggcgcaaa tcatcactac ccacagcagc ggcacggtag ccgttacacc atcgccaatc
     2101 gcgaccgcca gcggataagg ccacgcctcg cgcaagccgt aacccagcgt catccacagc
     2161 accggtaacg gtgacgcgac gagaatcgac cagaaaagag tgcgcaacgt cagccagaag
     2221 tgatcctgag tcactttgcc aactttcgcc gccgaacgtt caagaaaacg ggtgaaatag
     2281 cggcgcgagt aaatactgca accgaccaga atcaacgcgc caaacagcgg caaaattgtc
     2341 tctttgctgg tcagcatcat cacactggct ttgcccaact gactgaaggt gtccagcgaa
     2401 atgagacgac gcagatcctg ggcgatttcc agcggccagg cgatggtcat cgggcgcacg
     2461 tcagaggtcc agaacagata gcggtgcgtt gcttcgttca cctctttcag cgcatcctcc
     2521 agttgcccgt tggagacttt cagcttggtc agttccaata gtagcgtgtc gccaccctgc
     2581 aacaatgagt tcagcaactc acgctgagtg cgcagttgtg cttccagaat acggttttgc
     2641 tcggcggtca gcggctgacc gtcggcctga tggatttgcc gtagcagcgg ctgtttatta
     2701 agcagatcct cataacgtaa ccgttgcaca cgcaactgcg ccatttcggt atcaagctgt
     2761 tgtggttttg gcatttctgg cagccgtgcc acctgcgccc gcagcgcttc gccgagcaga
     2821 ttggacgatc ccagccattg cgactgttca cgcagcgtat tcaacgcctg ccggacctgt
     2881 aacgtctggc tggcagcctg acgctgttgc gaggcaacga gatccatccg ctgcgcctgt
     2941 tgattcaaag ccgccgatag ttcgcggtta attttgaatt gcgcgacgat atctttcggc
     3001 aaatcggcgc tgttttctgc cagcaattcg gtactttcca gcgcccgctc cgcctcaagc
     3061 tgacgttggc tgtttaattg attacgcaag gcctgcaaat acgcatccag ttgctggctc
     3121 tctttttccg ccagctctga gcgtaagcgc gctaattcct ggcggttatt ggcagacagc
     3181 tgcgccagct ccagttcatc aacgagcgcc ttaagacgtg cagagtcaga ctgcaacgcg
     3241 aaattttgtg cctgattgag cggagtattg ccggtaagcg ttcccaggcg gcgctcgatc
     3301 tcatttaact gacggcgggc gtcggtttgc tgttgcggca gttgattcag cgaatcggca
     3361 atctcgcggg tgcgctcctg ctcttgctgg gcctgacggc ttttatccag caactggctg
     3421 ctgacctgga gaatttcctg attcagcgcg tcggtagaca ttcccggcga cacgctgcgc
     3481 ggctcgtcac gcatgttgtt taattgtgcg cgcagagtag cggagagttt cggataatta
     3541 tcgataactt gctgatattg tttgatgcgc tcaagggaac cttttcgttc ctcaagcgca
     3601 tttaaggcag actggagcgc ctctacgact tccggctgtg cgggtttcgc cgcttttgcc
     3661 tgctccagtt cctgagtgat ttgtttgcta tcgggggccg tcgcggcgta cgccccccaa
     3721 ctgaggcacc aggccatcag aaaagtgata atcaggcgca cgtcagcgtt tcctttgatg
     3781 gattagacct ggtctttttt atcgtcaacc aatgggctgg cgtcgtgttc tgcttcgatc
     3841 tcttcagcag gaagtggggc gggttcagcg tctggcgtaa caaaggtttc ggtagatact
     3901 gccagcggct ggccaatttt cgtgacagac aggctttcca gttgctcaac cagattcact
     3961 ttacccggtg caaacaggtt gataacggtg gaaccgagtt taaagcgacc catttcctgg
     4021 cctttcagca gtgccacaga accgtcgttt tccccggcag gccaggtcca gcgcttgatg
     4081 ataccttcgc gcggcggcgt aatggtgccc gcccagaccg tctcaatgct gccaacaatc
     4141 gtcgctccga ccagaatctg cgccattggg ccaaattcgg tatcgaaaag gcaaatcacg
     4201 cgttcgttac gggcaaacag attcggcacg ctctgagcgg tgagatggtt aacggagaag
     4261 agatcgcccg gcacgtagat catctcacgc agaataccgt tgcacggcat gtgtacgcga
     4321 tggtagtcac gcggggagag gtaagtggtc acaaacgtac cgttgcggaa caggtccgcc
     4381 atcagatagt tgcctgccag cagggcttcg aggctgtagt tgtggccttt ggcttgcagg
     4441 attttatctt cttcgatttt acccaactgg ctgataacgc catcggcagg catgaccagt
     4501 acattcggat cggtatcgat tgggcgtact tcgtcacgca gcggacggac aaagaattcg
     4561 ttaaaggtgc ggtagctggc ggtgtccggc ttttgcgcct ctttcatgtc gaccttgtag
     4621 tatttaacga acagatcgat aaccagtttt gtcagccatc ctgcccgctt gcttgcgccc
     4681 caacccgcca ggcgagtaag ccatagtttc ggcagaatgt actgtagcga aagtttaaat
     4741 gaatttaaca aggtagcctc caggccattg ttttgtcgtt cctgatccgg ccaacatgcc
     4801 ggatcctgaa aaaaagggga cgattctaac gacggttagc ttaattgtca gtcatccgta
     4861 tcagaaaagt ttttacgcgt ttttacctgc gccatgcttt ccagaatacg gtgatagttt
     4921 tcgaaacggg tttccgcgat tttcccttcc tcaaccgctt cccggatagc gcagcccgga
     4981 tcggtatcgt gtttgcaatc gcgatattta cacagaccta aatagtcatg gaattcgaca
     5041 aagccctgag tgatttgttc cggctccagg tgccagaggc cgaactcacg cacaccaggg
     5101 gaatcaatca catcaccgcc gtgcgggaag tgatacagcc gagcggcggt cgtggtgtgc
     5161 tgaccgagcc ccgagttgtc agagacatcg ttggtcagga tctctttttg cagccccagc
     5221 aacgcattca gcaggctgga tttaccgacg ccagactgcc cggcaaaaat gctgatgcgc
     5281 ccggtcaacg cctcttccag cggttttagc ccatcctgag tatggctgga aaccatcaat
     5341 acgcgataac cgatattgcg gtagatatcc atctgctcgt tgacgaacgc catgccttcg
     5401 tcgtccagca ggtctatctt gttgagcaca ataatcggct caatctgcaa ggtttcgcag
     5461 gccaccaggt aacggtcgat aatattgagt gacagctccg gcaaaatggc ggagacaatg
     5521 acaatctggt cgatgttggc ggcaataggt tttacgccgt cgtagaaatc cggacgcgtc
     5581 aacaccgagg tacgctcatg caccgcttcc acgatccctt tgacatttac gccttccgcc
     5641 gccggtttac ccggacgcca gactacacgg tcgccggtca ccagcgaacg gatggtacgg
     5701 cgaatattgc agcggtgaac gtcgccatcg gcggattcca catcagcgtg cataccaaag
     5761 cggctgatga cgataccttc atcaggctcg ccaaacagat tgtcgtcgta gtcgggcttc
     5821 tccttagacg ttttaagacg acgctggtga ttggcgttca cgcggcgctg ctggcctttg
     5881 gagagtttat ttttactcaa tcgtacagac tcctggtcgc ccctgatggg caaaacatct
     5941 atgatacacg caattgtgga tcaatatagt cactgtgaat gggtggaaaa tagcatgagt
     6001 gccaatgaaa acaacctgat ttggatcgat cttgagatga ctggtctgga tcccgagcgc
     6061 gatcgcatta ttgagattgc cacgctggtg accgatgcca acctgaatat tctggcagaa
     6121 gggccgacca ttgcagtaca tcagtccgat gaacagctgg cgctgatgga tgactggaac
     6181 gtgcgcaccc ataccgccag cgggctggta gagcgcgtga aagcgagcac gatgggcgat
     6241 cgggaagctg aactggcaac gctcgaattc ttaaaacagt gggtgcctgc gggaaaatcg
     6301 ccgatttgcg gtaacagcat cggacaggac cgtcgtttcc tgtttaaata catgccggag
     6361 ctggaagcct acttccacta ccgttatctc gatgtcagca ccctgaaaga gctggcgcgc
     6421 cgctggaagc cggaaattct ggatagtttt accaagcagg ggacgcatca ggcgatggat
     6481 gatatccgtg aatcggtggc ggagctggct tactaccgcg agcattttat caagctgtaa
     6541 aatttttcct ggtcacgtaa gcgccggaat ccacttgcgg cggcgtgatt ttgacgctaa
     6601 attgaacgtc ttgctgataa tttcagcact tgagataaaa acgcaaaaaa aacttttttg
     6661 gggggttgca gagggaaaga tttctcgtat aatgcgcctc ccgtaacgac gcagaaatgc
     6721 gaaaattacg aaagcaaaat taagtagtac gcgggaatag ctcagttggt agagcacgac
     6781 cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc gctccaaaat ttgaaaagtg
     6841 ctgcaaagca cagaccaccc aagcgggaat agctcagttg gtagagcacg accttgccaa
     6901 ggtcggggtc gcgagttcga gtctcgtttc ccgctccaa
//
LOCUS       DAJTNN010000192         6840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTNN010000192 DAJTNN010000000
VERSION     DAJTNN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.194"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            40..246
                     /locus_tag="OO150_003611"
                     /pseudo
     CDS             40..246
                     /locus_tag="OO150_003611"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            991..2904
                     /locus_tag="OO150_003612"
                     /pseudo
     CDS             991..2904
                     /locus_tag="OO150_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3032..3754)
                     /locus_tag="OO150_003613"
     CDS             complement(3032..3754)
                     /locus_tag="OO150_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5517613.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            3901..6528
                     /gene="gyrA"
                     /locus_tag="OO150_003614"
     CDS             3901..6528
                     /gene="gyrA"
                     /locus_tag="OO150_003614"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5517614.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDAAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6677..>6840
                     /locus_tag="OO150_003615"
     CDS             6677..>6840
                     /locus_tag="OO150_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517615.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVMHNNLQIDL"
BASE COUNT         1642 a         1683 c         1974 g         1541 t
ORIGIN      
        1 ggctatggag atggtccttc cactgcggcg ggtggcttta tgtatctcgg cttaagtgaa
       61 gttacctttg atattgccga cggaaaaacg ctggttatcg gcaatacaga gaatgacgga
      121 gctgttgact ctattgctgg taccgggttg atcaccaaaa caggttccgg cgatctggta
      181 cttaatgcag ataacaatga ctttactggc gagatgcaga ttgaaaacgg tgaagttacc
      241 ctgggccgca gcaactccct gatgaatgtc ggcgatacgc attgccagga cgatccgcaa
      301 gactgctacg gtctgacgat agggagtatt gataagtacc agaatcaggc agagctaaac
      361 gttggctcca cccaacaaac ctttgtgcac tcattgacgg gctttcagaa tggcacttta
      421 aatatcgatg ctggtggcaa tgttactgtt aatcaaggta gttttgctgg caccatcgaa
      481 ggtgctggtc agctcaccat tgcgcaaaac ggcagctacg tgctgtcggg ggcgcagtcg
      541 atggcgctaa ccggcgatat agtcgttgat gatggtgcgg tgctttcgct ggaaggcgac
      601 gcggcagatc tcgccgctct ccaggacgat ccgcagtcga tcgtgttaaa cggcggtgtg
      661 ctcgatctct ctgatttctc cacctggcag agcggcacat catacaatga tggccttgaa
      721 gtcagtggca gcagcggaac ggttatcggc agtcaggatg tggtagatct tgcaggtggc
      781 gacaatttgc atatccgcgg cgacgggaaa gatggcgtct acgtggtggt cgatgcgagc
      841 gacgggcagg taagtctggc aaacaataat agttatttgg gcacaacaca aatcgcctcc
      901 ggtacgctga tggtgagcga caactcgcag cttggagata cccactataa ccgccaggtt
      961 atctttaccg ataagcaaca agaaagcgtg atggagatta ctgccaatgt cgatactcgc
     1021 tctacgacga ctgagcatgg gcgtgatatt gaaatgcgcg ccgacggtga agtggcagtt
     1081 gatgcggggg tagacacgca gtggggcgca ctgatggctg acagcagcgg gcagcatcag
     1141 gatgagggta gcacatttac taaaacgggg gcgggtacgc tggagctgac cgccagcggt
     1201 acaacgcagt cggcagtacg tgtcgaagaa ggcaccctga aaggcgatgt tgcggatatc
     1261 tttccttatg cttcgtcact gtgggtcggt gatggggcga cgttcgttac tggcgcggat
     1321 caggatattc agtctatcga tgctacttcc agcggcacta tcgatatcag cgatggtacg
     1381 gttttgcgtc tgaccgggca ggatacttcc gtcgccctta atgcctcatt gtttaacggc
     1441 gatgggacgc tggtgaatgc taccgatggc gtgacgttga caggtgagct taataccaac
     1501 cttgaaactg acagcctgac ctatcttgcc gacgtgacgg ttaatggtga tctgaccaat
     1561 acgtccggtg cggttagcct gcaaaatggc gtcgctggcg atacgctaac ggtaaacggt
     1621 gattatactg gcggcggtac gctactgttc gacagcgaat taaacggcga tgactcggta
     1681 agcgatcaat tggtgatgaa cggtaatact gctggcaaca caactgtggt ggttaactcc
     1741 attacaggga ttggtgagcc gacatcgaca ggcattaaag tggttgattt cgcagctgat
     1801 cccacgcagt ttcaaaacaa tgcacagttc agtctggctg gcagtggcta cgtcaacatg
     1861 ggagcgtatg actacacgct agtggaagat aacaacgact ggtatctgcg atcgcaagaa
     1921 gtaacgccgc catcgccacc tgatccagac ccggacccaa cacccgatcc tgacccaaca
     1981 cccgatccaa tccccgctta ccagccggtg ctgaatgcca aagttggcgg ttatctcaat
     2041 aacctgcggg cggcaaatca ggcgtttatg atggagcgac gcgatcacgc tggtggcgat
     2101 ggtcagacgc tgaatttacg tgttatcggc ggacgttatc attacacggc ggcagggcaa
     2161 ctggctcagc aggaagacac ttctacggtg cagcttagcg gcggcttgtt tagcgggcgc
     2221 tggggcacgg atggcgagtg gatgcttggg gcggttggtg gctacagtga taatcagggc
     2281 gacagccgct cgaatatgac cggaactcgc gccgataacc agaaccacgg ttatgccgtt
     2341 gggctgacct caagctggta tcagcacggt aattagaagc agggggcctg gctggatagc
     2401 tggctgcaat acgcgtggtt taacaatgat gtttccgaac atgaagatgg cgtggatcgt
     2461 taccattcgt cggggattat cgcctcgctg gaagccgggt atcagtggtt accggggcgt
     2521 ggtgtggtga ttgaaccgca ggcgcaggtg atttatcagg acgtgcagca ggatgatttt
     2581 accgccgcta accatgcgcg cgtgtcacaa tcgcaggggg atgatattca gacgcggctg
     2641 ggtttacaca gcgaatggcg taccgcggtt ggtgtcacac caacattaga tctgaattat
     2701 tatcacgatc cccatgcgac ggaaattgaa gaagatggca gcactatcag tgacgatgcg
     2761 gtgaagcgac ggggtgaaat aaaagtggga gtcacgggca atatcagtca gcgagtgtcc
     2821 ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt ttgcccagac ggcagggttt
     2881 ttgtcgatga cggtgaaatg gtaatcactg tctagtcggc acggttgccg gatgcggcgt
     2941 aaacgcctta tccggcctac ggggagcatt tgtaggcctg ataagacgcg gcaagcgtcg
     3001 catcaggcat tggctcggcc aaacaacaac ctcacttatt ctgcgtgtgc agcatatagt
     3061 tcacatccac gccggggccg agtttaaaag tattagtgag cgggttgtaa tgtagcccag
     3121 ttatatgtcg ctctttaagg cttgtctgat ctacccagcc aagcagttct gccggtttaa
     3181 taaacttctt cacatcatgc gtgcctttgg gcaccatgcg caaaatatat tccgcgccaa
     3241 ccaccgccat cagccatgac ttgccgttgc ggttaagcgt cgagaaaaag acatcgccgc
     3301 ctggtttcac cagttgcgca caggctctga ccactgactg cggatcgggg acgtgctcca
     3361 gcatctccat gcaggtcacc acatcatact gcccggcatg ttttgccgcg tgctcttcca
     3421 cggtttcctg cacataatcc acctgaatgc cgctttccag cgcgtgcagt tttgccacct
     3481 gcaatggctc aaagcccata tccagaccgg tcaccgtcgc gccttcgcgc gccatactct
     3541 cggccagaat gccgccgcca caaccgacat cgagcacctt tttgccaaat aaaccgccag
     3601 cacgctcggc aatatagccc agacgcagcg ggttaatgcg gtgcaacggt ttgaactcac
     3661 cttcaagatc ccaccagcgg gaggcgacgg cttcaaattt agcgatctct tcgtggtcta
     3721 cgttatggtt ttccggcgat ttttcggcat tcattggcac ttctactccg taattggcaa
     3781 gacaaacgag tatatcaggc attggatgtg aataaagcgt ataggtttac ctcaaactgc
     3841 gcggctgtgt tataatttgc gacctttgaa tccgggatac agtagaggga tagcggttag
     3901 atgagcgacc ttgcgagaga aattacaccg gtcaacattg aggaagagct gaagagctcc
     3961 tatctggatt atgcgatgtc ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc
     4021 ctgaagccgg tacaccgtcg cgtactttac gccatgaacg tactaggcaa tgactggaac
     4081 aaagcctata aaaaatctgc ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat
     4141 ggtgacgcgg cggtttatga cacgatcgtc cgtatggcgc agccattctc gctgcgttac
     4201 atgctggtag acggtcaggg taacttcggt tccatcgacg gcgactctgc ggcggcaatg
     4261 cgttatacgg aaatccgtct ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa
     4321 gagacggtcg atttcgttga taactatgac ggcacggaaa aaattcccga cgtcatgcca
     4381 accaaaattc ctaacctgct ggtgaacggt tcttccggta tcgccgtagg tatggcaacc
     4441 aacatcccgc cgcacaacct gacggaagtc atcaacggtt gtctggcgta tatcgatgat
     4501 gaagacatca gcattgaagg gctgatggaa cacatcccgg ggccggactt cccgacggcg
     4561 gcaatcatta acggtcgtcg cggtattgaa gaagcttacc gtaccggtcg cggcaaggtg
     4621 tatatccgcg ctcgcgcaga agtggaagtt gacgccaaaa ccggtcgtga aaccattatc
     4681 gtccacgaaa ttccgtatca ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg
     4741 gtaaaagaaa aacgcgtgga aggcatcagc gcgctgcgtg acgagtctga caaagacggt
     4801 atgcgcatcg tgattgaagt gaaacgcgat gcggtcggtg aagttgtgct caacaacctc
     4861 tactcccaga cccagttgca ggtttctttc ggtatcaaca tggtggcatt gcaccatggt
     4921 cagccgaaga tcatgaacct gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa
     4981 gtggtgaccc gtcgtactat tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt
     5041 gaagcattag ccgtggcgct ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg
     5101 ccgacgcctg cagaagcgaa aactgcgctg gttgctaatc cgtggcagct gggcaacgtt
     5161 gccgcgatgc tcgaacgtgc tggcgacgat gctgcgcgtc cggaatggct ggagccagag
     5221 ttcggcgtgc gtgatggtct gtactacctg accgaacagc aagctcaggc gattctggat
     5281 ctgcgtttgc agaaactgac cggccttgag cacgaaaaac tgctcgacga atacaaagag
     5341 ctgctggatc agatcgcgga actgttgcgt attcttggta gcgccgatcg tttgatggaa
     5401 gtgatccgtg aagagctgga gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa
     5461 atcaccgcca acagcgcaga catcaacctg gaagatctga tcactcagga agatgtggta
     5521 gtgacgctct ctcaccaggg ctacgttaag tatcagccgc tttctgaata cgaagcgcag
     5581 cgtcgtggcg ggaaaggtaa atctgccgca cgtattaaag aagaagactt tatcgaccga
     5641 ctgctggtgg cgaacactca cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat
     5701 tcgatgaaag tttatcagtt gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc
     5761 aacctgctgc cgctggagca ggacgaacgt atcactgcga tcctgccagt gaccgagttt
     5821 gaagaaggcg tgaaagtctt catggcgacc gctaacggta ccgtgaagaa aactgtcctc
     5881 accgagttca accgtctgcg taccgccggt aaagtggcga tcaaactggt tgacggcgat
     5941 gagctgatcg gcgttgacct gaccagcggc gaagacgaag taatgctgtt ctccgctgaa
     6001 ggtaaagtgg tgcgctttaa agagtcttct gtccgtgcga tgggctgcaa caccaccggt
     6061 gttcgcggta ttcgcttagg tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc
     6121 gatggcgcaa tcctcaccgc aacacaaaac ggttacggta aacgtaccgc agtggcggaa
     6181 tacccaacca agtcgcgtgc gacgaaaggg gttatctcca tcaaggttac cgaacgtaac
     6241 ggtttagttg ttggcgcggt acaggtagat gactgcgacc agatcatgat gatcaccgat
     6301 gccggtacgc tggtacgtac tcgcgtttca gaaatcagca tcgtgggccg taacacccag
     6361 ggcgtgatcc tcatccgtac tgcggaagat gaaaacgtag tgggtctgca acgtgttgct
     6421 gaaccggttg acgaggaaga tctcgatacc atcgacggca gtgccgcgga aggggacgat
     6481 gaaatcgctc cggaagtgga cgttgacgac gagccagaag aagaataatt ttacttcttc
     6541 atgccaaaag ggagctatct cccttgtttg aattgaaaag tccaggctgc aaagtctggg
     6601 cttttgtcgt attagggcac ggtaaagttt ggctgtgccc gtaaaaaatg gctggctata
     6661 cacaaggaat gtggcaatga gtggtgaaaa aaaggcgaaa ggctggcggt tctatggtct
     6721 tgtaggtttt ggcgcaatag tactgctttc cgctggcgtc tgggcgttgc aatatgctgg
     6781 cagtgggcca gaaaaaacgt tgtcgccgct ggtgatgcac aacaatctgc aaatcgatct
//
LOCUS       DAJTNN010000193         6804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTNN010000193 DAJTNN010000000
VERSION     DAJTNN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.195"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            190..2106
                     /gene="dnaK"
                     /locus_tag="OO150_003616"
     CDS             190..2106
                     /gene="dnaK"
                     /locus_tag="OO150_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5517616.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2195..3325
                     /gene="dnaJ"
                     /locus_tag="OO150_003617"
     CDS             2195..3325
                     /gene="dnaJ"
                     /locus_tag="OO150_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5517617.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3429..3581)
                     /gene="mokC"
                     /locus_tag="OO150_003618"
     CDS             complement(3429..3581)
                     /gene="mokC"
                     /locus_tag="OO150_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5517618.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            4167..5333
                     /gene="nhaA"
                     /locus_tag="OO150_003619"
     CDS             4167..5333
                     /gene="nhaA"
                     /locus_tag="OO150_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5517619.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5399..6298
                     /gene="nhaR"
                     /locus_tag="OO150_003620"
     CDS             5399..6298
                     /gene="nhaR"
                     /locus_tag="OO150_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5517620.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(6336..>6804)
                     /locus_tag="OO150_003621"
     CDS             complement(6336..>6804)
                     /locus_tag="OO150_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5517621.1"
                     /translation="RFNYNLNTILLVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHI
                     RFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDG
                     TNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT         1739 a         1601 c         1757 g         1707 t
ORIGIN      
        1 atttctgcgc aaaagcacaa aaaatttttg catctccccc ttgatgacgt gggttacgac
       61 cccatttagt agtcaaccgc agtgagtgag tctgcaaaaa aatgaaattg ggcagttgaa
      121 accagacatt tcgcccctat tacagactca caaccacatg atgaccgaat atatagtgga
      181 gacgtttaga tgggtaaaat aattggtatc gacctgggta ctaccaactc ttgtgtagcg
      241 attatggatg gcaccactcc tcgcgtgctg gagaacgccg aaggcgatcg caccacgcct
      301 tctattattg cctataccca ggatggtgaa actctggttg gtcagccggc taaacgtcag
      361 gcagtgacga acccgcaaaa caccctgttt gcgattaaac gcctgattgg ccgccgcttc
      421 caggacgaag aagtacagcg tgatgtttcc atcatgccgt tcaaaattat tgctgctgat
      481 aacggcgacg catgggtcga agttaaaggc cagaaaatgg caccgccgca gatttctgct
      541 gaagtgctga aaaaaatgaa gaaaaccgct gaagattact tgggtgaacc ggtaactgaa
      601 gctgttatca ccgtaccggc atactttaac gatgctcagc gtcaggcaac caaagacgca
      661 ggccgtatcg ctggtctgga agtaaaacgt atcatcaacg aaccgaccgc agctgcgctg
      721 gcttacggtc tggacaaagg tactggcaac cgtactatcg cggtttatga cctggggggt
      781 ggtactttcg atatttctat tatcgaaatc gacgaagttg atggcgaaaa aaccttcgaa
      841 gttctggcta ccaacggtga tacccacctg ggtggtgaag acttcgacag ccgtctgatc
      901 aactacctgg ttgaagaatt caagaaagat cagggcattg acctgcgcaa cgatccgctg
      961 gcaatgcagc gcctgaaaga agcggcagaa aaagcgaaaa tcgaactgtc ttccgctcag
     1021 cagaccgacg ttaacctgcc gtacatcact gcagatgcga ccggtccgaa acacatgaac
     1081 atcaaagtga ctcgtgcgaa actggaaagc ttggttgaag atctggtaaa ccgttccatt
     1141 gagccgctga aagttgcact gcaggacgct ggcctgtccg tatctgatat cgacgacgtt
     1201 atcctcgttg gtggtcagac tcgtatgcca atggttcaga agaaagttgc tgagttcttt
     1261 ggtaaagagc cgcgtaaaga cgttaacccg gacgaagctg tagcaatcgg tgctgctgtt
     1321 cagggtggtg ttctgactgg tgacgtgaaa gacgtactgc tgctggacgt taccccgctg
     1381 tctctgggta tcgaaaccat gggcggtgtg atgacgacgc tgatcgcgaa aaacaccact
     1441 atcccgacca agcacagcca ggtgttctct accgctgaag acaaccagtc tgcggtaacc
     1501 atccatgtgc tgcagggtga acgtaaacgt gcggctgata acaaatctct gggtcagttc
     1561 aacctggatg gtatcaaccc ggcaccgcgc ggcatgccgc agatcgaagt taccttcgat
     1621 atcgatgctg acggtatcct gcacgtttcc gcgaaagata aaaacagcgg taaagagcag
     1681 aagatcacca tcaaggcttc ttctggtctg aacgaagatg aaatccagaa aatggtacgc
     1741 gacgcagaag ctaacgccga agctgaccgt aagtttgaag agctggtaca gactcgcaac
     1801 cagggcgacc atctgctgca cagcacccgt aagcaggttg aagaagcagg cgacaaactg
     1861 ccggctgacg acaaaactgc tatcgagtct gcactgactg cactggaaac tgctctgaaa
     1921 ggtgaagaca aagccgctat cgaagcgaaa atgcaggagc tggcacaggt ttcccagaaa
     1981 ctgatggaaa tcgcccagca gcaacatgcc cagcagcaga ctgccggtgc tgatgcttct
     2041 gcaaacaacg cgaaagatga cgatgttgtc gacgctgaat ttgaagaagt caaagacaaa
     2101 aaataatcgc cctataaacg ggtaattata ctgacacggg cgaaggggaa tttcctctcc
     2161 gcccgtgcat tcatctaggg gcaatttaaa aaagatggct aagcaagatt attacgagat
     2221 tttaggcgtt tccaaaacag cggaagagcg tgaaatcaaa aaggcctaca aacgcctggc
     2281 catgaaatac cacccggacc gtaaccaggg tgacaaagag gccgaggcga aatttaaaga
     2341 gatcaaggaa gcttatgaag ttctgaccga ctcgcaaaaa cgtgcggcat acgatcagta
     2401 tggtcatgct gcgtttgagc aaggcggcat gggcggcggc ggttttggcg gcggcgcaga
     2461 cttcagcgat atttttggtg acgttttcgg cgatattttt ggcggcggac gtggtcgtca
     2521 acgtgcggcg cgcggtgctg atttacgcta taacatggag ctcaccctcg aagaagctgt
     2581 acgtggcgtg accaaagaga tccgcattcc gactctggaa gagtgtgacg tttgccacgg
     2641 tagcggtgca aaaccaggta cacagccgca gacctgtccg acctgtcatg gttctggtca
     2701 ggtgcagatg cgtcagggtt tctttgccgt gcagcagacc tgtccacact gtcagggccg
     2761 cggtacgctg atcaaagatc cgtgcaacaa atgtcatggt catggtcgtg ttgagcgcag
     2821 caaaacgctg tccgttaaaa tcccggcagg ggtggacact ggagaccgca tccgtcttgc
     2881 gggcgaaggt gaagcgggcc agcatggcgc accggcaggc gatctgtacg ttcaggttca
     2941 ggttaaacag cacccgattt tcgagcgtga aggcaacaac ctgtattgcg aagtcccgat
     3001 caacttcgct atggcggcgc tgggtggcga aatcgaagta ccgacccttg atggtcgcgt
     3061 caaactgaaa gtgcctggcg aaacccagac cggtaagctt ttccgtatgc gcggtaaagg
     3121 cgtcaagtct gtccgcggtg gcgcacaggg tgatttgctg tgccgcgttg tcgtcgaaac
     3181 accggtaggc ctgaacgaga agcagaaaca gctgctgcaa gagctgcaag aaagcttcgg
     3241 tggcccaacc ggcgagcaca acagcccgcg ctcaaagagc ttctttgatg gtgtgaagaa
     3301 gttttttgac gacctgaccc gctaacctcc ccaaaagcct gcccgtgggc aggcctgggt
     3361 aaaaataggg tgcgttgaag atatgcgagc acctgtaaag tggcggggat cactccccgc
     3421 cgttgctctt actcggattc gtaagccgtg aaaacagcaa cctccgtctg gccagttcgg
     3481 atgtgaacct cacagaggtc ttttctcgtt accagcgccg ccactacggc ggtgatacag
     3541 atgacgatca gggcgacaat catcgcctta tgctgcttca ttgctctctt ctccttgacc
     3601 ttacggtcag taagaggcac tctacatgtg ttcagcatat agggggcctc gggttgatgg
     3661 taaaatatca ctcggggctt ttctctatct gccgttcagc taatgcctga gacagacagc
     3721 ctcaagcacc cgccgctatt atatcgctct ctttaaccca ttttgtttta tcgattctaa
     3781 tcctgaagac gcctcgcatt tttgtggcgt aattttttaa tgatttaatt atttaacttt
     3841 aatttatctc tttatcgcaa ttattgacga caagctggat tatttttgaa atattgtcct
     3901 aacaaataac gccgactgac aagaaattaa ttattacttt tcctaattaa tccctcagaa
     3961 atcctcacct taagctatga ttatctgagc ttagggtcac tcgtcagcgc ttacagccgt
     4021 caaaaactca tctcaccgct gatggcgaaa attctacaaa ggctcgtaaa aaacgaatta
     4081 ttcctacact ataatctgat tttaacgatg attcgtgcgg ggtaaaatag taaaaacgat
     4141 ttattcacct gaaagagaaa taaaaagtga aacatctgca tcgattcttt agcagtgatg
     4201 cctcgggagg cattattctt atcattgccg ctatcctggc gatgatgatg gccaacagcg
     4261 gcgcaaccag tggatggtat cacgactttc tggagacgcc ggttcagctc cgggttggtt
     4321 cactcgaaat caacaaaaac atgctgttat ggataaatga cgcgctgatg gcggtatttt
     4381 tcctgttagt cggtctggaa gttaaacgtg aactgatgca aggatcgcta gccagcttac
     4441 gccaggccgc atttccagtt atcgccgcta ttggtgggat gattgtgccg gcattactct
     4501 atctggcctt taactatgcc gatccgatta cccgcgaagg gtgggcgatc ccggcggcta
     4561 ctgacattgc ctttgcactt ggagtgctgg cgctgttggg aagtcgtgtt ccgttagcac
     4621 tgaagatctt tttgatggct ctggctatta tcgacgatct tggggccatc attatcatcg
     4681 cattgttcta cactaatgac ttatcgatgg cctctcttgg cgtcgcggct gtagcaattg
     4741 cggtactcgc ggtattgaat ctgtgtggtg tacgccgcac gggcgtctat attctggttg
     4801 gcgtagtgtt gtggactgcg gtgttgaaat cgggggttca cgcaaccttg gcgggggtaa
     4861 ttgtcggctt ctttattcct ttgaaagaga agcatgggcg ttctccagcg aagcgactgg
     4921 agcatgtgtt acacccgtgg gtggcgtatc tgattttgcc gctgtttgca tttgctaatg
     4981 ctggcgtttc actgcaaggc gtcacgctgg atggcttgac ctccattctg ccattgggga
     5041 tcatcgctgg cttgctgatt ggcaaaccgc tggggattag tctgttctgc tggttggcgc
     5101 tgcgtttgaa actggcgcat ctgcctgagg gaacgactta tcagcaaatt atggcggtgg
     5161 ggatcctgtg cggtatcggt tttactatgt ctatctttat tgccagcctg gcctttggta
     5221 gcgtagatcc agaactgatt aactgggcga aactcggtat cctggtcggt tctatctctt
     5281 cggcggtaat tggatacagc tggttacgcg ttcgtttgcg tccatcagtg tgacaggacg
     5341 gtttaccggg gagccataaa cggctccctt ttcattgtta tcagggagag aaatgagcat
     5401 gtctcatatc aattacaacc acttgtatta cttctggcat gtctataaag aaggttccgt
     5461 ggttggcgca gcggaggcgc tttatttaac tccacaaacc attaccggac agatccgggc
     5521 tctggaagag cgcctgcaag gcaaattatt taagcgcaag ggacgtggtc tcgaacccag
     5581 cgagctggga gaactggtct atcgctatgc cgataaaatg ttcaccttaa gccaggaaat
     5641 gctggatatt gtgaactatc gcaaagaatc caatttattg tttgacgttg gcgtggctga
     5701 tgcactttcc aaacgcctgg tcagtagcgt gctgaatgct gcagtggtag aaggcgagcc
     5761 gattcatctt cgctgcttcg aatccaccca cgaaatgcta ctggagcaat taagccagca
     5821 taaactggat atgatcattt ccgactgtcc gatagactct acgcagcagg aaggcctgtt
     5881 ctccgtgaga attggcgaat gtggcgtgag tttctggtgt acaaatccac caccagaaaa
     5941 accgttcccg gcttgtctgg aagaacggcg acttttgatt cctgggcgac gttcaatgtt
     6001 agggcgcaaa ttgcttaact ggtttaactc ccagggatta aacgtagaaa tcctcggcga
     6061 gtttgatgat gccgcgttga tgaaagcttt tggtgcaatg cacaatgcaa tcttcgttgc
     6121 cccaacgctt tatgcatatg acttttatgc cgataaaact gtcgtagaaa ttggtcgcgt
     6181 cgagaatgtg atggaagagt accatgctat ttttgctgag cggatgattc agcacccggc
     6241 ggtacagcga atctgcaata cggattattc tgcgcttttt agtccagcgg cgcgttaatc
     6301 ggcagctccc ccaaagttaa agtggaggag atagattagt tgtacataac cacgattttg
     6361 actcggctca ttatttgccc gcttgagaca ttatttccat atgtacgcgg gcgaataaat
     6421 agaggaatct gattacttcc ttcatgggga tgctgaaaag agtagtaatt gcttttacca
     6481 tactggattt cattcgtacc atcattggag agttgaaagc ctacttgacc cgcaccgtcc
     6541 tcactgtttt ctaactcatt ttttaatatg ctggttcctg actcctgtat cgtcactggt
     6601 tcaaagtaat attcaacatc actgggttca aaaactaatt caccttgctc gccctggatg
     6661 cagtcaaatc ttatatgatg aaactgataa ttttcattat tgatacgaga cagtacaaat
     6721 cgtccagtgt cgatggattg atcttcaaaa ccacaggtca ttaaatttac cagtaaaata
     6781 gtatttaaat tataattaaa gcgg
//
LOCUS       DAJTNN010000194         6801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTNN010000194 DAJTNN010000000
VERSION     DAJTNN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.196"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            175..903
                     /gene="mpaA"
                     /locus_tag="OO150_003622"
     CDS             175..903
                     /gene="mpaA"
                     /locus_tag="OO150_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5517622.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPSSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(878..1843)
                     /gene="ycjG"
                     /locus_tag="OO150_003623"
                     /pseudo
     CDS             complement(878..1843)
                     /gene="ycjG"
                     /locus_tag="OO150_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
     gene            1962..2468
                     /gene="tpx"
                     /locus_tag="OO150_003624"
     CDS             1962..2468
                     /gene="tpx"
                     /locus_tag="OO150_003624"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5517623.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2512..4053)
                     /gene="tyrR"
                     /locus_tag="OO150_003625"
     CDS             complement(2512..4053)
                     /gene="tyrR"
                     /locus_tag="OO150_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5517624.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4201..5262)
                     /locus_tag="OO150_003626"
     CDS             complement(4201..5262)
                     /locus_tag="OO150_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5517625.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREICRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5259..6656)
                     /locus_tag="OO150_003627"
     CDS             complement(5259..6656)
                     /locus_tag="OO150_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5517626.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
BASE COUNT         1625 a         1936 c         1662 g         1578 t
ORIGIN      
        1 acgacttaca tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa
       61 cttccggcaa aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata
      121 atccgcaaaa tttgcacaac actgcctgta atacttcctg aaccagagac gatcatgacc
      181 gtaacccgcc cacgcgccga acgcggcgca tttccgcccg gaacagaaca ttacggacgt
      241 tcattattgg gcgcgccgtt aatctggttt cctgcccctg cagccagccg cgaaagtggt
      301 ttgattctgg ctggcactca cggtgatgaa aactcttcgg tcgtcacgct ctcctgtgct
      361 ctgcggacgt tgacaccttc tttgcgccgc catcatgtgg tgctgtgtgt gaatcctgac
      421 ggctgccagc tggggttacg ggccaatgct aatggtgtgg atttaaacag aaactttccg
      481 gcggcgaact ggaaggaagg tgaaacggtt tatcgctgga acagcgccgc tgaagaacgt
      541 gatgtcgtat tactgacagg tgacaaacct ggctcagaac ctgaaaccca ggcgctgtgc
      601 cagcttatac accgcattca acctgcctgg gtggtctcat tccacgatcc actggcctgt
      661 attgaagatc ccagacacag cgaattaggc gaatggctgg cccaggcgtt tgaattgcct
      721 ctggtaacca gcgtcggtta tgaaacgcct agctctttcg gcagttggtg tgcagatctc
      781 aacttgcatt gcatcaccgc agagtttccg cccatctcct ccgacgaagc cagcgaaaaa
      841 tatctctttg cgatggcaaa cttgctgcgc tggcatccta aagatgcaat tcgcccgtcg
      901 tgaactgaag cgccggttcc acatctaccg ccagccaggt cggtccgtca agatcggcga
      961 aactgacctg cggcaccagc ggtaaagcgg cgctaatggc acgagaggta cacaacatgc
     1021 agcccagcat cagactgaaa ccttgtgcac gcgcttcagt cgccagcgcc agcgcttccg
     1081 tcagaccacc ggttttatcg agcttaatgt taaccatctc atagcgccct ttcagcgcct
     1141 tcagattgct acgtgtatga caactctcat cagcacaaat cggcaacgga tgaataaaat
     1201 tctccagcgc cgcatcgtcc tgcgcaggaa ggggttgttc aagcatcgca acgcctaaat
     1261 ccgccaatag ctggcaacgc gccgccaacc cttatgcacg ccaggattca tttgcatcaa
     1321 cgatcagcgt cgcatcgggc acagctgtgc gaattgccac catccgctca ctaataagat
     1381 ggttatccag cttcactttc agtaatttcg cgcctgcctg ccagagtgtt gatgcactat
     1441 tggccatctg atcaggcgta ccgatgacta ccgtctgtgc agtgataact gtctcgggaa
     1501 gcgttatgcc gatcaaatca gccagcgatt gctgctgttt tcgcgcggcc agatcccaca
     1561 atgcacaatc cagcgcatta cgtgctgcgc cggcagggag aattttttgc aactcctccc
     1621 gtgtcagccc tttctctaat tgcgacacga cgctcataat ttgcgccatt accgaggcat
     1681 cactttctcc ataacgcgga tacggcgtgc attcgccggt gcctttaata ccctcttctt
     1741 ccagttcaac caccaccacg cgcgcttcac tgcgacttcc ccgggcaatc acaaacgggg
     1801 tatgtaaggg ctaggcttcc tcaaatacct taacggttct catctttact ccttgttgtc
     1861 gcggtggtct gcggattaag cgcaaatagc gtttgctgtg ttattgacag ttagcataaa
     1921 ctaggtgtga cgttaactat atgtaaacag gaaagataat catgtcacaa accgttcatt
     1981 tccagggcaa cccggttaca gtcgccaatt ccatcccgca ggcgggtagc aaagcgcaga
     2041 cttttactct cgtggcaaaa gatctgtctg acgtcaccct cggtcagttt gcgggtaaac
     2101 gcaaagtgct gaacattttc ccgagtattg ataccggtgt ttgcgccgca tcagtacgta
     2161 agtttaacca actggcaacc gagatcgaca acaccgttgt gctgtgtatt tctgccgatc
     2221 tgccgttcgc ccagtctcgt ttctgcggcg cagaaggtct gaacaacgtt atcaccctct
     2281 ccactttccg taacgctgaa tttctgcaag ccttcggtgt ggcaattgct gatggcccac
     2341 tgaaaggtct ggcagcgcgt gccgttgtgg ttattgacga aaatgacaat gtgattttca
     2401 gccagctggt ggatgaaatc accaccgagc cggattacga agcagctctg gctgtactga
     2461 aagcataagt taatatgcct gatggtgttg caccatcagg catattcgcg cttactcttc
     2521 gttcttcttc tggcttaagc catattcccg caacttattg gcaatcgcgg tatgtgaaac
     2581 gccgagacgt tttgccagtt tgcgcgtgct gggataattg cgataaagct gggttaatac
     2641 cgagcgttca aaacggctgg tgatttcgtc cagcgaacct tccatcgcat cttcgcccac
     2701 ggctaccgtt gcggcgtcat aatccggcaa caaaatatcc tgtggacgca gctcataacc
     2761 gtccagttgt gtcagtgcgc gatatatagc gttctttaac tgccgcacat ttcccggcca
     2821 cgcataacgc gtaagtacag tattcaggtc agcggccagt ttcggacgcg gcacgccctg
     2881 ctcgtcggca aagcgggcga cgaacagctc agttaacggc atgatgtcct gcggacagtc
     2941 acgtagcggc ggcagattga gcgtcaacac gttcagacga taatagagat cttcacggaa
     3001 catgcctttt tgcaccagtt cgaccagatt cttctgcgta gcgcaaatca cccgcacatc
     3061 gacatgcacc tcatggtctt cgccaacccg acggaaagtg ccatcattaa gaaaacgcag
     3121 taatttcgcc tgcatccgtg gtgacatttc ccctatttca tccaacagca ccgaaccacc
     3181 gttcgcctgc tcaaagaatc ctttcttccc ttccggagca tgaccaaaca gttcactctc
     3241 gaccgcatct tccggtatag acgcacagtt cagcgccagg taaggtttgc ccgctctggg
     3301 gcttgcctga tggcaggcgt aggcaaagag atctttacct gtacctgtgt cacccgtaat
     3361 cagcagcggc gcgcttagca tcgccagttt ctgcgcctgt tcgacaacat gcttcatttt
     3421 cgggctgacg gcgacaattt gactgaaggc gctgacgtcc tgggcggcga cattttgcaa
     3481 ctggcggccc atacgaatcg ttgatcgcaa catcaccacc gcaccggtca ggacgtgttg
     3541 atcattttca tcctgaagat aaacaggcgt aatctccatc aggaaattct gcccattaat
     3601 aacgacatgc tcgttatgcg aatcttgcgg ttcgctttcc agccaacgta aaaaattaaa
     3661 gccgttaatc aattgtgcgg cggtatggtt gcgcaggcga tccaattttt gcccaaaaag
     3721 ctgacagctc gccgggttcg ccatatccac tttgcttttc atatcgacag agagcacagg
     3781 ttcgggcaac gcctccagta acgcgctcaa cgccagatgc tcacgttcgg aaggcatcca
     3841 cgggacagta cgcacatcgg taacacctgc aatacggcgt atttcggcca tcagactgct
     3901 gaaactctca aactccagtt cagcaaaatt gaggtagatt cgcccaatgg gatcaatctc
     3961 aataccgcgt aaatcaatgc ctcttagcac gagtagatcg agtaattcgc gggtcagacc
     4021 gagtcggtct tcacaaaaga cttccagacg catgggaacc ttcacctgaa aaaagaacaa
     4081 ttaatatgat gatatgacac tatttggata gcaggaaggt ttctgtcaac aatcattgac
     4141 acaaagacgg aaaaacgggc acttcggcgt aaagatatcc ggtcagcacc cgatattgca
     4201 ttatttttcg ctgggcgttt tgcctttttg cagcgtttct ttcacctgac cgataagctg
     4261 acgacggaaa tccccgaggc gaggtttgtc atcgtcaatc cacggcagcg ggcggcaaag
     4321 ctccatagct ttaatcccga gtcgtgccgt cagaagtcca gcaccaattc cctgagctgc
     4381 gcgggtagac aaacgagcag cgagatcttg cgacatccag tccatcccca cttcgcgcac
     4441 cagttcgctg gctccggcaa aagcgatatt cagcaatacc agcttaaaca gacgcaaacg
     4501 gctgtaatac cccagttcaa tgccatacag cgtggcgatg cgattaatta aacgcagatt
     4561 gcgccaggcg ataaacgcca tatcgaccaa cgccagcggg ctgaccgcaa tcatcaacgt
     4621 tgattccgcc gccgaacggc agatttcgcg ccgcgcctgg gcatctaaaa ctggctggac
     4681 caaatgcgca tacaaactga ccacttcacg gtcgttttgc gtttcatgga ttgaggcata
     4741 ccagcgttgc agcgctggat gagactgatc aatacccgcc tgctgcgcca gtttttcgca
     4801 aaatgcgcgg cctttgcccg tgccgtggct gtgcaacaaa tcgcgcgctt cgtcgcgttc
     4861 atgggcgcgc tgtcgcaagc gccataagcg ccgccactct gttaccacag aacctacgcc
     4921 agcgccgata atcaatgccc cagcggcaca tccacccagc gccacccagt cctgagtttg
     4981 ccaggcattc attgtccact gtacaccctg cccgacaacg cttgcgccaa acagagccag
     5041 cccgcccatc accattttgc gccacaggct gcgtttcgga cgtaacgctg catccattac
     5101 cgcttcaact tgcccctctt cttcaggcgc ttcatcgagc gtggccgggg caaaattttg
     5161 cgcctgattt tcgtcaaagg tctgctgcgc cctgaatttt ggattctgat cgacctccag
     5221 cggaccgtcg aaatcaatac gtggttttaa cggttcggtc atcgcaattt atctcctatt
     5281 aaaaattcca gcgcggcatc aagacgaata tgcggcagcg gtttgtcgac atccatcacc
     5341 tgcgggcgaa acgcttcaaa ctggaaccct tgtttgtccc agaacgcctg accgggcaaa
     5401 cgcgcaggaa cttcaccggg ataaacggtg agtggcgcac catcgctgag ccggttaccg
     5461 cgcaatgccg gaattttctc gccgttgacg tcaataatgc cgctggtggt cgcctgcacc
     5521 gaggccagcc ccaggcaatc catactaatc ccttcaaacg ccgcattttg ccaggcatcc
     5581 tgaatcagtt gttgcagtaa tgaaaccata ttggcgtgct gatcgatggt cacatggtcc
     5641 gctttagtgg cagcaaacaa tagcttatcg ataaccggcg aaaacaaacg ccggaacaag
     5701 gtacgctgcc cgtagtggaa actttgcatc agctgcgtca gtgccagacg catatcatta
     5761 aatgcctgtg gcccactgtt gagaggttgc aggcaatcaa ccagcacaat ctggcggtca
     5821 aagcgcagaa aatgattctt atagaacccc ttcaccacct tctcgcagta ataattaaac
     5881 cgctcgcgca gcattccggc attggtgtgc ttatcggcct gcgccagttt ggactcgccc
     5941 caggtatcga catccggcca cgggaagaat tgcagcgcgg gcgcacctgc catatctcct
     6001 ggcaagacaa agcgccctgg ctgaataaag tgcagcccct gctgtttaca gtggtggaga
     6061 taatcggtcc acgcggcggc aatgtctgcc agccggtttt cgtcggcagg tgctagcggg
     6121 tccagccctt cgcacatcat tcgccatttg accgaccatt cgccgcgctg accattgagt
     6181 aagcccgtca tctggcgtga ccagcttaaa tagtcctgcg ccagcatcgg caggtcgagc
     6241 aaccattcgc cgggataatc cacaatctcc agatacagcg tggaggtgtc cttgaagtgg
     6301 cgtagcagcg aatcgttcga tttaaaacgt agcgccaggc ggatttcact gacgccgcgc
     6361 gttggcgtcg gccaggcggg aggatcgcca tataactgcg ccagtccttc atcatatgtg
     6421 aagcgcggaa tgccaaagtc acgctgagga atgcgtttta cgcccagcag gcgctcttca
     6481 cgcaccgcgc ttaacagcgg caagcgtgct ccggcgtgaa tattgagcaa ctgattgacc
     6541 atagcagtga taaacgctgt tttgccgctg cggcttagtc cggttacggc gaggcgcaga
     6601 tgtctgtcga caccccgatt caccagcgca ttaagttcat ttttaagtcg cttcattgct
     6661 gtcctgtgct gctctgaaga ccagaaaaaa atcattcgta aggatatgaa tgtgggggcc
     6721 aggccattga ttatcaactg cccgtgttgt catcgacggg cgaactccat aagatgtgca
     6781 gcattcacgt tcggacggag t
//
LOCUS       DAJTNN010000195         6775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTNN010000195 DAJTNN010000000
VERSION     DAJTNN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.197"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..200)
                     /locus_tag="OO150_003628"
     CDS             complement(<1..200)
                     /locus_tag="OO150_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5517627.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQW
                     VTAARKGLNTPGSRTVAELESEV"
     gene            complement(890..2599)
                     /locus_tag="OO150_003629"
     CDS             complement(890..2599)
                     /locus_tag="OO150_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5517628.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(3031..3750)
                     /locus_tag="OO150_003630"
     CDS             complement(3031..3750)
                     /locus_tag="OO150_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5517629.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3875..4438)
                     /locus_tag="OO150_003631"
                     /pseudo
     CDS             complement(3875..4438)
                     /locus_tag="OO150_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4422..4680
                     /locus_tag="OO150_003632"
                     /pseudo
     CDS             4422..4680
                     /locus_tag="OO150_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4789..5367)
                     /locus_tag="OO150_003633"
     CDS             complement(4789..5367)
                     /locus_tag="OO150_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517630.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKDLYKCV"
     gene            complement(5788..6025)
                     /locus_tag="OO150_003634"
                     /pseudo
     CDS             complement(5788..6025)
                     /locus_tag="OO150_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6212..6641)
                     /locus_tag="OO150_003635"
                     /pseudo
     CDS             complement(6212..6641)
                     /locus_tag="OO150_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1843 a         1555 c         1252 g         2125 t
ORIGIN      
        1 acttcagatt ccagctcagc cactgtgcgg gagcctggtg tattgagccc ttttcttgcg
       61 gcggtaaccc attgtcctaa agtgccttca ggaacagata atcgggaagc gccttcactg
      121 atcgaaagct gattttcagg aaccgttttg acggcttcgg ctttaaactc cgtagagtaa
      181 cgttgggttt ttctgctcat tattggctcc ttctgatgcc attctatttc aggaaggagt
      241 gtccgttaaa ctcaggctac ctcagataag cttgatacaa ctggtctggt atatacagtt
      301 tctttgagtg cggttacgtc cacagaggta acggtaaaag taaccccttg tcgggaccac
      361 tccgaccgcg cacagaggca ttttcatcgc tgacaggccg cattgcctcg cagggctgga
      421 ggaggatcgg tgatatccgg acgaccgtac cggcttacgc cgggcttcgg cggttttact
      481 cctctgccat gtgcaacaac ggagtgccgc cttacatgcc gctggcgcgg catatcctgg
      541 taacattttt gttgatacca ccttccggcc ttatcctgac cagtgggctt tcctggcatc
      601 gatgataccg atgaacgcgc atgatattga gccgacgata ttacgggcaa ctggaaatac
      661 tcaccctctg gatgtgacct ttatccatga agaagatttg gccataccat ggaaacctga
      721 acaatcatca gtttacgccc atgtaaaccc ctacgggata tttgaactcg atatggaaac
      781 aaggttgcca atcgaggtgg ttgcctgaaa gttggcagtt acccggtcac tacaccttgt
      841 tggagtgaac atatcaaaaa caggtagtta cacaatttaa attacagtct cataaaaaat
      901 gattaaatct atttattatt tcaataaacg tttttttcat cttctttgaa aattgttctg
      961 ctatagtatc cggttccaga ttgttctctt ttattattct acaatactct aatattgttt
     1021 ctttgtctct atttactaca gatacaaata caccaggaat accatcttta ttagttgcgg
     1081 acagcatttc gaccaatcgt cttgtggtaa aactatagtt ttttagaata ttacaaaagg
     1141 cacgtattgt ttcggtatgg ccattataaa atgccataaa aagtcctgat gtaccgttgg
     1201 gattttttat ccataataat tcatctatat attcctggtg aggggggagt atttttagta
     1261 tatctccata cgccataatg gcctttgtgt gcccattttg aagtgccata aacaacccat
     1321 gctttacttt caataattct ttaatttgtt cactactaag cattttctta ttaagcagac
     1381 ttccgaacat acgaatacta tctgcatgtc cattctgcaa tgcaaaaaat aacccaggta
     1441 aatcatggct gtctttcgca gctaatagat ataatctctt atcggaagtt aacggggttt
     1501 taaagactat ttccccatag gcttttattg tgtctacatg tccgttcata aaagacatgc
     1561 ataaacccgg cacattgttt gcccctaccg cagcaagcaa tgtaagtatt gtttcttgac
     1621 taagattgca catatttaat atcttaccat atacttgaat aatgtctgaa catccgtttt
     1681 gaagtgctat ataaaatgca ggagtaccat cagagcttct cgtggccaga aggttcacca
     1741 gtttagattg agaaatttca ccatttttgt atttttccat cagtataaat aatacttctg
     1801 tcagattctt tgttatcgca tgatatataa ctctggaatt gatcaattca tctggtaaaa
     1861 gtttcactgt gtttgttaga ggggaattat gcctatcttc aaatattacc tgcccgggtt
     1921 gctccagacc ataaattttc caataaaaat catcaaaaaa atccttagcg gataattgac
     1981 tcagttcgaa atggctatca caagtaaata cagtacgttt atgagtaaca gttcgattag
     2041 gatcgaaaaa ctgaacaacc catttttcac tttcttgtac gcgctttatt ttcaacccta
     2101 acgccatttg gtggtttaat gtgcatgctg ataatgttct gacaaagtct ccattttttt
     2161 tcatgttata aaaaaaatta gtaataactg ttccccactt tttattttcc acaaaataaa
     2221 tataagctgg tgattttgaa aaatcttccg ttttttccaa acatacattt tttgcaatag
     2281 catcaggggt gctaagaagc tgatagttta ccttcccatt tgtttccata aaattcattt
     2341 cccaatagga agctaggtga cggcaaacta tttgtcttcc atatatatct tttgccactc
     2401 catttatatt taccttcaca tttctctttt ctgaaaaata aggtgctgtt ttattcttaa
     2461 actgaattag ttcactggag tttgagaccc tcataataga acttgttatt gttctattat
     2521 ctctgttaac agacatatct gttgataacg aattttttgt tgaaaatata ctagatgaaa
     2581 atgttttatt taacggcata gaagacaacc atagagatgt tataatacta ttagattgca
     2641 gttactaata gtaaaagcct ccttacaagg aggtagctta ttcagaatgc tcctataaga
     2701 tagcgagctc gtcaacgata cagatgatct gattatattg tgcaaaagga gaacaggaaa
     2761 ataggcactg gaaaagatga aggcctgatg ggagcattaa ctaatgcaaa atccgacagg
     2821 ttaatatccc gaaaagcaat caaacgactc aaacaggaga tgcacgctga agtggtggac
     2881 aactgttcgg atttcagatg agtagctcag cagtttatga gccgaatgct ctgtcacggt
     2941 caggaatcag gctgcatacg ctgtgccaaa tctaatcgta gttattctaa ccagttttcc
     3001 agctacaagg tggtgtagct ggcatcttct ctactctatc atcaaacgat aaaatggttc
     3061 ctctcgtaac atttgctctt gcctttcact tccatctcga tcgcttcgta gttcattacg
     3121 atagctgaca tatttccagt cttcaggtct aacatctgac gccggatact ccccgggagc
     3181 gattttgctt cttaatagat tagactcgag acacattata aattgtcgta ttttatgaag
     3241 taataaagca ctatattgcg agcattcctg atctgatttt acatatagcg taaactgagc
     3301 tcctatcccc acacgagatt gctgagaaac tcgattcata tcagttatct tccatttatc
     3361 tataggactg tcctctgaaa ataataaacc agacaaaatt tggaatgcca gaggaacttg
     3421 ctccctagct atactaatat gaaacttgtc cccaacaaaa tcccccttac tctgaggatt
     3481 ttcacgacat gcatgaataa acacatctcc actatggttc acaatctgga atccttggta
     3541 catgacagaa aaaaattgac atatatcaga gtaagttggc acgttcgatg ctactttgaa
     3601 atagtacgct gggtattgcc gtatttggtt gtacaatata ttgaagttat ttttcaatct
     3661 ttcattcgca gaaagcatct gcggtatttc agacctcaca acattcaaag aatctaaatt
     3721 tagttttaga cagggctttt ttatgggcat agaaaacgtc ctctataaaa tagatatatt
     3781 atcttatatt gattgtttat catcttttat aaaacgatac attatatgta atgtatgtaa
     3841 gcgtaagggt cttctccgat catggtggga aggcttagaa cgccatattc agtttcccat
     3901 agtggaacat cactcccagc ttaaagcgct cccggttgcg gtagcccttg gcttttattc
     3961 tcagcagcct gatcttgctg ttcagtgctt ccgcatttcc gtttgatacg ccatggcgca
     4021 tggcgttcag gataccgtac aaccttttcc ctatcgtttt agccacattc ttcatcatgg
     4081 ggacgtcact gttggccgcc agcgacagcc atctctgcca gtcgttgcgt ctttcctcgc
     4141 tccatggtct gctccagata tcctttgcca gttctttcag cgcccagcac aggctcgtca
     4201 gcttcatctg cgcacgcagc catatcagct tttcctgcct ggattcggtc atccatttat
     4261 cgctgtgctg ccacaggaag cgagttccct ttgcctgacg acgactttca acaggaagat
     4321 gcggatgttc attctggcgg gttttatcca ccacctcacc cggttgtttc gccacatgaa
     4381 agcgatcgaa ggctattttc tcaaccgcat tgggtaagtg gatgcgcgct gctcttagtt
     4441 tgctgagcca gtttagcatt gccttctaca tgaccgatgc ctgagccggt ataccgaact
     4501 ttattgtcct caaccagtca tgttataagc aagaaatgca ctcagcggat agagcgacat
     4561 aatctgaacc tgcggacaca tcttaagcgg cttacccgca aaaccatatg cttctcaaag
     4621 tctgatgata tgcactataa gatcatcggc tggtatctga caatcaacca ccaccactaa
     4681 atctgcgtca cgacctgcaa ctgagtctgg ttgaacaggc tgaggccctg ctgaagcaac
     4741 gcgaacctct cgactcttgt tggattttac caaatagttt ttctaaaatt aaacacattt
     4801 atataagtcc tttgctgaac aaaacgcagc ccgccatcct ttttcattca taaattcagc
     4861 aatggcagat aaaacttttt catcaagcat ataaaaaata atgcaagtag actcaatatg
     4921 cctcatacaa ataaggccgc acatatccgt aatatatttg tcaatgctgc tataatttgc
     4981 atggtaatat gccatgctat tatctgatga ttttacatat gcagtatgac atatttttcc
     5041 ttcacgatta acaaaactga caaaaccact ttcaccagga gtaatatata aagatgaatt
     5101 taacgtgcgc tcactaatat tcattaattg ttgaaattca atggaatagt tttcagttaa
     5161 tgtatctatt atcttggctt tgtctttctt tgttatctca ttgtcttttt cggcaagaaa
     5221 aattgccact ttatgacatg cattcagctc gttaatttta cctgataaaa ctgcttttct
     5281 taccacatcg ttgaactcat cgatagtgtg ttttttatta tatatatcat gaaaaatata
     5341 gcctaaagac ttaaacgaga ctttcatttt atattctcct taaatattaa caggatatat
     5401 atatcacatt ttcctctata ttttcagaaa ccagctatca ttttccccat tactggtacg
     5461 tcgcttcaga tatttccgca gcaagcgata accctgcgca ctatgaggat gccattcatt
     5521 ataatgtttg aacaccttcg cgagactact ttactgctgt taacacgtca ggttcgaaca
     5581 taacactgat ttaatcatat ttcatcgttt tgacgaagtt ttctgccatt ccattttgca
     5641 ccgccgtgtg tttgggggca aggccgacca tctgtgtttc attcgccaga tataagtgat
     5701 gagatggcat tctccctcct tcacggcaga agcattttga ccgtttaggg gatgcggacg
     5761 attttcatgc ctgctaagat actttcgtta ctgatgctgt ccgttttcca ccatttcatc
     5821 atcactgatt ggcgttgtgt ttttggtcag cactccagct ttacgtttct cttttaaccg
     5881 gtaactctct cctttgatat tcagcgtgta gcagccgatc taggatcgct gttgccagca
     5941 cgtgatctcc gaacatttct ccccagtctg cgaccccttt gtttgacgtc agtatgatgc
     6001 tcgctttttc atatcgacag ttcatcgata ggaatttaaa tccccaaaag attaaaaaag
     6061 caccgaagaa tgcgcgttta ttcaacacaa cctgatcatc aagtaatcgg tatagacact
     6121 taatattgct tacattgatt gattcaatgt atctctgatg gttttttgct ccgttcccct
     6181 caacatggac gcaacttccc ctctgcgact ctcagccgca ccaccgtatt cgagccagcg
     6241 gctcatgcat cataacctgc tctgccagac ggtaacccct cttcagcccc gtaaaccgca
     6301 tctgacctcc gcacagctcg cacttcagcg ggtcaacctt cagtcactgc tgatacatcc
     6361 ctctccttgt gatttgcctc gccgtttttc tcactgtctc cgttatggcg tacaccacat
     6421 cttccagtaa tcgccgcttc accggactca ggaccccgta tcaccatccg gaacccctta
     6481 tccgccacat gccaggagaa cctttccata aactcgtcgc cactcatcac aaggttttct
     6541 tctcgttttg tccggtggct gttgtaacgc aggccaattt catcctgacc ggcataatgt
     6601 tccaggcggc tcatctgcac cggctgcttc tccagccagt taagccatcg gtttccctgc
     6661 tgaaaaatat cggcaaaacg gggaagcatc agaaaggcgg aataacgcca tccggccagt
     6721 gaaccgtacc gcattcaggg caatacatgc cgtcggcgtt gataccggca ggaat
//
LOCUS       DAJTNN010000196         6698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTNN010000196 DAJTNN010000000
VERSION     DAJTNN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.198"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            167..274
                     /locus_tag="OO150_003636"
     CDS             167..274
                     /locus_tag="OO150_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517631.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(327..1085)
                     /locus_tag="OO150_003637"
     CDS             complement(327..1085)
                     /locus_tag="OO150_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5517632.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVTYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1372..2301
                     /gene="pfkB"
                     /locus_tag="OO150_003638"
     CDS             1372..2301
                     /gene="pfkB"
                     /locus_tag="OO150_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5517633.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2402..2692
                     /gene="ghoS"
                     /locus_tag="OO150_003639"
     CDS             2402..2692
                     /gene="ghoS"
                     /locus_tag="OO150_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5517634.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2798..3658
                     /locus_tag="OO150_003640"
     CDS             2798..3658
                     /locus_tag="OO150_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5517635.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3699..4235)
                     /locus_tag="OO150_003641"
     CDS             complement(3699..4235)
                     /locus_tag="OO150_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5517636.1"
                     /translation="MTYQQAGHIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4382..5050
                     /gene="hxpB"
                     /locus_tag="OO150_003642"
     CDS             4382..5050
                     /gene="hxpB"
                     /locus_tag="OO150_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5517637.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5213..5791
                     /locus_tag="OO150_003643"
     CDS             5213..5791
                     /locus_tag="OO150_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5517638.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKH"
     gene            5936..>6698
                     /gene="tcyP"
                     /locus_tag="OO150_003644"
     CDS             5936..>6698
                     /gene="tcyP"
                     /locus_tag="OO150_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR5517639.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNL"
BASE COUNT         1728 a         1619 c         1628 g         1723 t
ORIGIN      
        1 attttcgata tatatgttga tgcataatat tattttttca taaaacgcag aaacagtgac
       61 caggtgtaca acgccaccca acggctgtaa taacaaaaaa tcccttgatg cccgtccctt
      121 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggaatgc cgactaaacg
      181 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
      241 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
      301 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
      361 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtaac gttgtaagtc
      421 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
      481 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
      541 catgcataag ccccggaggc atagcccagc ggctgagttt cactggcatt gtcggtatat
      601 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
      661 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
      721 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
      781 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
      841 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
      901 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
      961 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1021 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     1081 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     1141 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     1201 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     1261 atatatattt atctgcaaaa ttttaaataa agctccaata aatcatattg ttaatttctt
     1321 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     1381 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     1441 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     1501 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     1561 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     1621 gaagccaaag actggacccg gcagaattta catgtacatg tggaagcaag cggtgagcag
     1681 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     1741 caagttctgg aaattgaatc cggggccatc ctagtcataa gcggaagcct gccgccaggt
     1801 gtgaagctgg aaaaattaac ccaactgatt tccgcttcgc aaaaacaagg gatccgctgc
     1861 atcgtcgaca gttctggcga agcgttaagt gcagcactgg caattggtaa catcgagttg
     1921 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccggac
     1981 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     2041 tcccttggtc cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca
     2101 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     2161 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     2221 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     2281 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     2341 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     2401 tatggccagt ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt
     2461 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     2521 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     2581 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     2641 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     2701 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     2761 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     2821 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     2881 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     2941 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3001 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     3061 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     3121 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     3181 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     3241 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     3301 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     3361 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     3421 tttcgaccct gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca
     3481 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     3541 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caaggttatt
     3601 tggcggtcag catttggtta ttgctcagca gtcactggat agattattag cagcatgata
     3661 tgggttgaag ataatggccg ctctgtgcgg ccttttgatt aaataaaccc aagcagagaa
     3721 aagaacacat aacctgcagc aatacaaata accggcagga tatacagtga gaaaaactgc
     3781 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     3841 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     3901 gcctggcgac tcattcgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     3961 aaaatcaccc agaacataac gttcacgcca ccattaaaat tgggcgtcgg ggagttatac
     4021 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag
     4081 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     4141 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     4201 aaaacagcaa tgtgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     4261 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     4321 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     4381 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     4441 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat
     4501 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     4561 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     4621 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     4681 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     4741 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     4801 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     4861 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     4921 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     4981 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     5041 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     5101 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     5161 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     5221 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     5281 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     5341 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     5401 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     5461 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     5521 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     5581 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctag ttccccaaaa
     5641 gggcaaccaa ctggaacgtt ttatctgtat ggcattattt gtctggtcgg tatggatgcc
     5701 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaataatca ataccttgca
     5761 gatccagttt catcggctta ttaagcatta ggttgaatac taaaaagaca aaaatcacct
     5821 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     5881 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     5941 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6001 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     6061 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     6121 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     6181 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     6241 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     6301 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     6361 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag
     6421 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     6481 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     6541 gaaactgctg aaggatgatg cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct
     6601 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     6661 ggctctaatg accaaagtgg ttgcaggttc taacttgc
//
LOCUS       DAJTNN010000197         6653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTNN010000197 DAJTNN010000000
VERSION     DAJTNN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.199"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(538..1068)
                     /locus_tag="OO150_003645"
                     /pseudo
     CDS             complement(538..1068)
                     /locus_tag="OO150_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1411..2061)
                     /gene="zinT"
                     /locus_tag="OO150_003646"
     CDS             complement(1411..2061)
                     /gene="zinT"
                     /locus_tag="OO150_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5517640.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2318..2953)
                     /gene="msrQ"
                     /locus_tag="OO150_003647"
     CDS             complement(2318..2953)
                     /gene="msrQ"
                     /locus_tag="OO150_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5517641.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(2954..3958)
                     /gene="msrP"
                     /locus_tag="OO150_003648"
     CDS             complement(2954..3958)
                     /gene="msrP"
                     /locus_tag="OO150_003648"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR5517642.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4067..4480)
                     /gene="hiuH"
                     /locus_tag="OO150_003649"
     CDS             complement(4067..4480)
                     /gene="hiuH"
                     /locus_tag="OO150_003649"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5517643.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4613..5284
                     /gene="hprR"
                     /locus_tag="OO150_003650"
     CDS             4613..5284
                     /gene="hprR"
                     /locus_tag="OO150_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5517644.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5284..6642
                     /gene="hprS"
                     /locus_tag="OO150_003651"
     CDS             5284..6642
                     /gene="hprS"
                     /locus_tag="OO150_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5517645.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASLGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYLNKHNVFRITLPQ
                     RN"
BASE COUNT         1882 a         1352 c         1511 g         1908 t
ORIGIN      
        1 cttacttttc ctaaacagaa caatcgtaga atattcttga agggttagat cgtcactgtt
       61 ttctgttcga tactgtgaca ttcagcactt gattcgctat ggatctgaca ggaagctttc
      121 gagcgaaaat gtgcagttat tcagtcgttt tcttatcgct caccattatt cttttagaca
      181 ttcatcctat gaaactgccg caaagttggt ggtgggaact gaagttgcgt agagaagggg
      241 tcaataattg agtcaaaaaa ggaacgcggc cgtcagatta aagccgcggg acaaagtcca
      301 taatttccaa gagttgttga atttcccagc ttttttgaaa gcctttgtat catactaaca
      361 agtaaaaaag gtttaaatac caaaattaac ctgtattttt gataagtctt accattgtac
      421 gatttacaca gtttctgctc ctccttgttg atagtggaaa gggtgcattg tgcacttgcg
      481 taaacattta tgacgcgttg atgtccatgg ttcacatttc cttgtcaggg gatcgactta
      541 tttgcggcga ggcatcattc gtaacagagt attatctttc tgaatgtagt gatgaaagag
      601 tgctgcgcca gcgtgagctg cgattaaaaa gtaacctata tttgcccagg tttcgtgaat
      661 attttatatc agtgctttaa tttcgctgtt tggggtaaca aagggagaca cattgaaacc
      721 aaggaaactc cacgattttc cactgtaagc catcaaagca atccccagca gaggaagtgc
      781 aaggaaggtt atataaagca tgatgtgcat tagcgaagcc gctttcattt gccaggcagg
      841 tggcggtggc acaatagaag gatcactata acggtgtttt ataatcaggc gtgaaaacat
      901 taacacccaa acgaatatac ccgcattgta atgtgtttct cgcatcagca gataagtact
      961 actaccttta ggaaaccagc cacggagttc catcgcggca taggttattg caacgaaaag
     1021 cagcgttatc caatgtaaat aaatttgagt ttttgagaat cggttcattt ttgtagctcc
     1081 tgtaattttt acagtcaagc tatcccacga accttaaaaa ttccttatac agatgtggtt
     1141 ttaagaattt gttatttaaa aaattgatag tgcacattgt aaccagccaa tataagcaat
     1201 ggcgtgagca caggtatctt tctctgttgg ccgtattgtg tatggaatcc gttattggtt
     1261 aactcaatgc gggttagctt attaatatac agacacgtca tagtgatgag cgtgtctgta
     1321 gctagcttgc gttcagtggc gtttgtggat ggaccagtgg cagttatgat gacttactaa
     1381 agcggcatcg aggcgttatc atgagaatac tcaatgagac atcatttcct cgaccacttc
     1441 ttcgctactc aactgatatg gataatacgt tggccagttt tccatttcat ttaataaaga
     1501 ttgctgtgaa tcattaccca taaaaatgtg aaaatgagat gattttcgtg gtgcaattat
     1561 atgatcgcta aattgtatat atttaggggc tttgctttca ggatctttac attcgaataa
     1621 gtaacgaacg cctttcttgc ctgatttata ggtgagtatt ttgtatccat cgtaatcata
     1681 tttacaggat gttgtttcat tatttctatg gaattcaaca atgccgtcct caatgccaat
     1741 catctcgata tctgttgcat aacctttgtg ataataatct ttaatttcag caaatgtttt
     1801 agttttatct gcatccgctt tcttctgaaa gacggggtca agtttgccac tttgcagtaa
     1861 aggataaacg gattgccaga ccccatccca gtcactgagc gttcggtttt gtacattggc
     1921 atcatcaaaa acaccattag cagctttttg ttcgacctct gttaagggtt tgccgtgtga
     1981 gtgatgacca tgcgaaaagg caggagcgct aacaataaag acacctaacg caacagccag
     2041 tttgtgaaga cgaatcgcca aaacagtttc ctccagagtc attgtatatg atgtgtaatg
     2101 ttatattgta acatatggca aaatgcaaca actcttagga gatgagagca tagttttctc
     2161 aggtgtactt atctatctta ttgaaataaa atgaattata ttgataattt gaggttgatg
     2221 acaaacgaaa cataatctga tgtgatctgc gtaggccgga tagggcgctc gcgccgcatc
     2281 cggcagggat aacgcgtact ttgtcggcaa tatgaagtta tacagataat ttgttgtgaa
     2341 cctgcttgcg taaccggtta aaaagggaac gcaacttctt ataccgtaag gctaaaagca
     2401 gtacagccag cccagcgtag atgagcggct gcggtgaaat aatcttcact gaccacagat
     2461 aatgtattgg ggccaggatc gcgacaagat agacgaaatt atgcaactgt tgccagtgct
     2521 tgccgagttt tcgctgcatc gcctgggttg aagtaaacgc taaagcaagc aaaataaccc
     2581 agctgataat tcctaacgtt aaataaggcc gggtaattaa ctccttgcct aataacgcca
     2641 gattattcac gcccagctcc agcaatgcgt aactggttaa atgcagtgtc gcccaggcaa
     2701 agcaccataa gcctaacagg cggcgagtgc gtatcaataa cggctgtttt gcgtagcgtg
     2761 ccagaggtgt gattaacaag gtcgccagca aaaatttcag tgcagtgcga ccggtaaaat
     2821 gctgaatatc tttcaccgga tcggcaccca gtccaccgtg attgatcgcc cagaccagcc
     2881 agagaaacgg caacaatccg gcaagatgca ggctaacttt cagccatgtc acctgttttg
     2941 ctgtcagacg cattcagaaa ttctcccgca aatccaggcc acgatacagc gatgccacct
     3001 ggtcggcgta accattaaac agtagcgttg gctggcgctg aacatcgagg atgccgcctg
     3061 aaccaataaa tcgttcggta gcctgtgacc agcgcgggtg atcaacatgc ggattaacgt
     3121 tggcgtaaaa accgtattcg tcaggcgctg ccagattcca ggtggttggc ggacgctcgc
     3181 gggtcagctt aatactgact atcgatttaa tccctttaaa gccatatttc cacggcacaa
     3241 tcagtcgtac cggcgcgcca ttttgtggcg gtaacgcctt gccataaaca cccacggtca
     3301 tcagtgtgag cggatgcatt gcttcgtcga gacgcaatcc ttcgacataa ggatatttca
     3361 gcccgccgcc gataaagcgg tcctgctggc ctggcatttg ttccggtgca taaattgttt
     3421 cgaaagcgac atacttcgca ttgctggtgg gttcggcaag cgccagcaat ttgtgcagcg
     3481 gaaaaccaat ccacggcacc accatcgacc atgcctccac gcagcgcata cgataaatac
     3541 gctcttccag cgggaagcga cgggttaaat catcgtgatc gagggtcaat ggttttgcca
     3601 cttcgccgct gattttcagt gtccatggat cggttttcag gctaccagca ttagcggcgg
     3661 gatcggcttt atccagcccg aattcataga agttgttata accggagact ttatctactg
     3721 gcgtcagtgg caggttattt tgccaggcgg caggcttgct gaactccagc ggttttccgg
     3781 cgggggcggg cgggcgatcg ttccctttaa accagctaag cagatcggca tgcgcagcgt
     3841 gaggcaaaga aagtgcagct gcactgatgc ccagtgcttt taacacctgc cgacgcttca
     3901 taaagaatac cgactcggcc gtaacatctg attcttttaa aaattgattc tttttcataa
     3961 gccatcacac ttttttgtcg ttagccagca agagccgttt actgttaatc tgtcagggag
     4021 tgtcctccct aacagtagca gagatctgct tttacatatt tatgcattaa ctgccacgat
     4081 aggttgaata cccatattgg ctaagtaata aaggcacatg ataatgctcg ttcactttat
     4141 taatatgaaa ttcaacgggg atctcaggga agaaactttc aagattttgt ttcttgaaat
     4201 agtccccggt tttaaatacg acacggtaat cgcccgtagt tgcagtttgc tcgggccaca
     4261 gtgctttaat tcgtccatcc ttatctgttt tggcggtatt aagttgtaac cagccgttat
     4321 ccgccttctt ttcaagagtg actgtcacgt cagcagcggg ctttccggtt tgctggttca
     4381 aaatgtgcac gctaagaatg ttttgttgtg cggcataaac caaagaaggt aatgaaaatg
     4441 ctgccgttgc taccgagagt actaaataac gctttaacat gtttatatcc ttgtcatgtg
     4501 aatgagtttt gaggatagcg aaaaagcggc cagggtaacc ttacagcagc attacaattt
     4561 tgtaatgaac caggctgttt ctataacata tgatttatgg catattattt tcatgaagat
     4621 tttacttatt gaagataatc aaaggaccca ggagtgggta acgcaggggc tttccgaagc
     4681 gggttatgtc attgatgccg tttctgatgg cagagatggg ctttatcttg cgctgaagga
     4741 tgattatgca ttgataattc tggatattat gcttccgggt atggatggct ggcagatctt
     4801 acaaacgtta agaacagcaa agcaaacccc tgttatttgc cttactgcaa gggattctgt
     4861 cgatgacaga gtcagagggc tggacagtgg agcaaatgat tatctggtaa aacctttttc
     4921 attttctgag ttgctggcaa gggttcgggc acaattaagg caacatcacg ctttgaattc
     4981 aacattagaa atcagtggct taagaatgga ctctgtaagt caaagtgtga gcagggacaa
     5041 tatcagtatt acactgacgc gcaaggagtt tcagttactt tggctactgg cctccagagc
     5101 tggcgaaatt atacccagaa cggttattgc gagtgaaatt tggggaatca actttgatag
     5161 tgataccaat acggtggatg tcgccattcg caggctccgc gcaaaagttg atgatccttt
     5221 tcctgaaaag ctaatcgcca caatccgggg gatgggctat tcattcgtag cggtaaaaaa
     5281 ataatgaaaa gattgtctat aaccgtccgt ttaaccttgc tttttatatt gctgctgtct
     5341 gttgctggcg ctggaattgt ctggactctc tataatggcc tggcaagtga gttgaaatgg
     5401 cgcgatgata caacactcat taaccggaca gcacagatca agcagttgtt aattgatggg
     5461 gtaaatccag atacgttacc tgtatacttt aaccggatga tggatgttag tcaggatatc
     5521 ttgatcattc atggtgatgg catcaataaa attgttaacc ggacaaatgt cagtgatgac
     5581 atgttaaata acatacctgc tagtgagaca atcagcgcag ctggcattta cagaagcatt
     5641 attaatgata ctgagattga tgctttacga attaatattg atgaagtttc gccatcatta
     5701 acggttactg tggctaaatt ggcttcagct agacataaca tgcttgaaca gtataaaatt
     5761 aatagcatta taatttgcat tgtcgccatt gtactttgct cagtattaag tccgctgtta
     5821 atcagaacgg gattacgaga gatcaaaaag ttgagtggtg taacggaagc gctgaattat
     5881 aacgatagcc gagagcctgt tgaggttagc gcattaccga gagaactaaa acctcttggg
     5941 caggcgttga ataaaatgca tcaagcctta gtcaaagatt ttgaacgcct aagtcaattt
     6001 gctgacgatc tcgctcatga acttagaacg cccattaatg cattactggg tcagaatcag
     6061 gttacgctca gtcaaaccag aagtatcgct gaatatcaaa aaacaattgc cggtaacatt
     6121 gaagagttgg aaaatatttc gcggttaaca gagaacatac tgtttcttgc ccgggcagat
     6181 aaaaacaatg ttttggtgaa actggactcg ctttctctca ataaggaagt cgaaaatttg
     6241 ttggattatc ttgaatatct ttcagacgag aaagagattt gctttaaggt cgagtgcaat
     6301 cagcaaatct ttgcggataa aattttactg caacgaatgt tatcgaatct tattgttaat
     6361 gccattagat attcaccaga aaaatcgcgt attcatataa ccagttttct tgataccaac
     6421 ggctatctta atattgatat cgccagtctt ggaacgaaaa tacatgagcc tgaaaaactc
     6481 ttccgtagat tttggcgggg agataattcg cgtcattccg taggtcaggg acttggtctt
     6541 tctttagtca aagcgattgc cgaattgcat gggggaagtg cttcatatca ctatctcaat
     6601 aagcataatg tgttccgaat tacgttaccg caaagaaatt aacatagggc ttc
//
LOCUS       DAJTNN010000198         6394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTNN010000198 DAJTNN010000000
VERSION     DAJTNN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.200"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..209)
                     /locus_tag="OO150_003652"
     CDS             complement(<1..209)
                     /locus_tag="OO150_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR5517646.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNS"
     gene            688..2466
                     /locus_tag="OO150_003653"
     CDS             688..2466
                     /locus_tag="OO150_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCR5517647.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(2506..2985)
                     /gene="xdhC"
                     /locus_tag="OO150_003654"
     CDS             complement(2506..2985)
                     /gene="xdhC"
                     /locus_tag="OO150_003654"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5517648.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2982..3860)
                     /gene="xdhB"
                     /locus_tag="OO150_003655"
     CDS             complement(2982..3860)
                     /gene="xdhB"
                     /locus_tag="OO150_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5517649.1"
                     /translation="MFDFASYHRAATLADAINLLANNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAAMSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3871..6168)
                     /gene="xdhA"
                     /locus_tag="OO150_003656"
     CDS             complement(3871..6168)
                     /gene="xdhA"
                     /locus_tag="OO150_003656"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5517650.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAIAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
BASE COUNT         1595 a         1511 c         1617 g         1671 t
ORIGIN      
        1 gagttgtcgc ggaatacgga aatacctaat ccactattaa agactttggt tgagatgttt
       61 tcagcacgca gtgcttttaa tgctgcggca acgtccagta cttgtttcag ttcatctggc
      121 gtctgttccc acgttaacaa aaaatctttc tcgtgaaggt gagaggtcag cgaattgata
      181 tccttaatca gctcattaac agttttcatc ataaagtccc ggtagtggaa tgacaggcaa
      241 agtggtttat ttcactgtga cttgccaaag tgattggtgt tttcacagct atacctggga
      301 ctaacaataa gtgtgccaga attatccgcg cggctgcaaa agactaattt gacggataat
      361 gtgtggggtt gatcacaaat tgatgccagc ttctggttgt ttgtaaaata cgaaaacgtt
      421 tggctttttt tatcgcgtta tttatttgct gctaagtatc agaaataaga taaaggggca
      481 ggctatctct cttcagtttg ataaactcac cgccgtaatt ctcatattga tatagaaatg
      541 tgatatctct tccataattt cattagaaaa tgataaaaat cttatgtttt tatcaaagcc
      601 aatttcgcct gagttaatat catcatgatc tgtgaaccat caacgtcttc aatgatcgac
      661 ttttaagtaa gtctggaagg taaagctatg gagcttgcta ctacgcagtc agtattgatg
      721 caaattcaac cgacaattca gcgttttgcc agaatgcttg ccagcgtttt gcagcttgag
      781 gttgagatcg ttgatgaaaa cttgtgtcgc gtcgccggaa cgggcgcgta tgggaagttt
      841 cttggtcgcc agttgagcgg caactcacgc ctgctccgcc acgtcctgga aacgaaaact
      901 gaaaaagttg tgacacagtc tcgcttcgat cccctttgcg aaggttgcga tagtaaagaa
      961 aattgccgtg aaaaagcatt tctgggtacg cctgtcattt tacaggatcg ttgtgttggg
     1021 gtgataagtt tgattgccgt tacccacgag caacaagagc atatcagtga taatttacgc
     1081 gaattttctg attatgttcg ccatatatcc accatttttg tttcgaaact tctggaggat
     1141 caggggccag gagataacat cagtaaaata ttcgcgacca tgatcgataa tatggatcag
     1201 ggcgtattag ttgttgatga tgaaagtcgg gttcagtttg ttaatcagac tgccttaaaa
     1261 acacttggtg ttgtacaaaa taatattatt gggaaaccta tccgtttcag accattaaca
     1321 tttgagagta attttactca tggacatatg cagcatattg tttcgtggga cgataaaagt
     1381 gaattaatca ttggtcaatt gcataacatt cagggccgac aattattttt aatggcattt
     1441 caccaatcgc ataccagttt ttctgtagca aatgcacctg atgaaccaca tattgaacaa
     1501 ttggttggcg agtgccgtgt tatgcggcaa ttaaaacgac tcattagccg tattgcaccc
     1561 agcccatcca gcgttatggt ggttggtgaa agcggcacgg gtaaagaagt cgtcgcccga
     1621 gcaatccata agttgagcgg aagacggaat aaacccttta ttgctatcaa ctgtgccgcg
     1681 attccggagc agcttctgga aagcgaactg ttcggttatg ttaaaggcgc atttactggc
     1741 gcttctgcca acggtaaaac agggttgatt caggcggcga atacgggcac gctgtttctc
     1801 gatgaaatag gtgatatgcc attaatgttg caggctaaat tactgcgcgc tattgaggcg
     1861 cgtgaaattc tgccgattgg tgccagtagc ccaatacaag tcgacattcg catcatttct
     1921 gcaactaatc agaatttggc ccagttcatt gccgaaggta aattccgcga agatctcttc
     1981 taccgactta atgttatccc gataactctg ccaccgctgc gtgaacgtca ggaagatatt
     2041 gaactattgg tgcattactt tttacatctg catacccgtc gtctgggatc ggtttatcct
     2101 ggcattgctc ccgatgtcgt cgaaatattg cgtaagcatc gttggcccgg aaacctgcgc
     2161 gagttaagca atttgatgga atatctggtt aacgtagttc cttcaggtga agttatcgac
     2221 agcacgctat tgccgccaaa tctgctgaat aatggcacaa cggagcaaag tgatgtaaca
     2281 gaggtcagtg aggcgcacct gtcactcgat gatgcgggcg gcacggcgct ggaggagatg
     2341 gaaaagcaaa tgatccgcga ggcgctttca cgtcataaca gcaagaagca agttgctgat
     2401 gaactgggca tcggcattgc tacgctctat cgcaagatta agaaatatga gttgttaaac
     2461 acataacctc agtgaatccg gcctgaattc aggccggata tccttttact tcgttttctc
     2521 gcaatccaga actgtattta caatcatctg ataccccgtg cagcgacaaa gatttcccgc
     2581 cagtccgcga cgaatttccg taatggttaa tggcttctcg cgtggtttcg ccagcattgc
     2641 cgtggtagcc ataatcaggc caggcgtaca aaacccgcac tgcactgcgc cggatttcgc
     2701 ataagcctgc tgaacatgag aaagttttcc gcctttcgct tcaccttcca gcgtgcggat
     2761 ctcttttcct tcagcccagg cggcaaggta taagcaactg tctattgctg tgccgtcgac
     2821 caacaccgta caggcaccac attcacccac gcagcaccct tgtttgacac ttagcagtcc
     2881 ttgttcgcgg agtaattccg agagcggcgt gcctggtgcg gcgtgaagct gaaaaggcat
     2941 cccgttaatg gtgcattcga tggtaattgt ttcgctgtga ttcattgcaa ttttcccccc
     3001 gccgcggcga cggcttcgct aatcactttt ttggtcatcg tctggatgag atgcagacga
     3061 aactctttac tggcccgcca tgaagaacgc ggggcgacat cttgcaggac agattcgctg
     3121 atagcttcca gcgtttgcag gtttaatggc gcattttgtg cagtctgttc ggcatgttgg
     3181 cagcgaatcg gcgttggcgc ggcaacacca aatgccaggc gtaattcgct gaaattgccg
     3241 ttatccagtc ggcaatgtgc ggcgcagcca atcgttgaaa tatccattgc gtcgcgcatg
     3301 gcatatttaa aatgcgcgct gcccgcgtgt tctttcggct gtggcggaaa atgaaaggcg
     3361 acgaggattt cgtcatgctc aagagacact ttgcccggcc cggtgtgaaa gccattaatc
     3421 gggacgaaac gaacaccgcg cggggagtgg atctccagtt tcgcgtcata aattagcgtt
     3481 ggcgtggcag aatctgcgct ggtggcaccg ttgcaaatat ttccaccgta ggtagcgacg
     3541 ttacggatct gcagtccagc aatggacatg gccgcagcac ataacgccgg gagatgacgt
     3601 tgagttatag gatcttctat tagctgggta aatgtcgttg cagagccgat acgtagcgag
     3661 ccatcttccg ccagcgtaat tccccgcagc tccgccagat tatgaatatc aacaatatgg
     3721 cgataacggt cattgtgatg gtggagctga atcagtacgt cagtgccacc ggcgagcagt
     3781 ttggcctgcg ggttgttagc cagcaggttg atggcatcgg caagggttgc tgcgcgatgg
     3841 taagaagcaa aatcaaacat gatgttatcc tcaaatcaat cctgccagat ggaactcttc
     3901 atataaccgt tttggcgtca gcggcagtgt attgattgca acaccggtag ccatcttcac
     3961 cgcgttacga atagcagcgg caacaggaat tattggtggc tcacccagtg acttatgtcc
     4021 gtatgcggat tgcggctcat tgatttcgac gaacgcgctt tccagttgtg gcagatccgg
     4081 catggtcggc attttgtaat ccagcagatt ggggttacgg accacgccgc ttttagcatc
     4141 gatgatcatc tcttcaaata gcgcccagcc aatgcccatt cccattccgc cgtgtacctg
     4201 accttctgcc agcagtggat taagaatatg ccctgaatca tgaacgttga ggatgcggtt
     4261 gatggtgact ttgcacagcg caatatcgac cgtcagatca acaaaggtac agccaaacgc
     4321 cggtgggtta gtggtggttt tgatggagct ttcagcagag agctgcccgc cgcgttcagg
     4381 gtggtagaaa gcgtccatcg ccaaatcttt taacgacatt aacggctctt ccggtcgttc
     4441 aaccagcacg atatggcctt ttatcagggt cagattcatc gctgactgat gtagcatgac
     4501 tgcggcgtga gcgatgattt tctcttttaa taatagtgcc gcactgcgca gcgcaggcgc
     4561 ggcaacatag ctctggcgtg aggcaaatgt gccgggatcg aacggcgtaa cgtcggtatc
     4621 ttgagttgaa ataacgcgaa cgtcgctgac cggaaccccc acggtttctg ccaccatttg
     4681 cgagaagacg gtgtcggcac cctgaccgat ttccgtcgcg ccgctttgca cgttgatggt
     4741 tccatcctga ttcatcagaa ggcgcgcgcc tgctatttct acgccgacag gccaggtgtt
     4801 agaggtgtag ctaaaacagg cgacgccaac gccgcggcgc aaattgcctt gctggttctg
     4861 gcattctgca cggcgttttt cccattcaaa gattttccgg cctttttcaa gacactccgg
     4921 caaccctgcg ctgtaaatac gtttgcccgt gagcggatta gcatctcctt cgcgggcggc
     4981 gttgcgtaaa cgaatttcaa caggatcaat acctaacgct gtcgcggcgt catcaagcat
     5041 agactcaacg gcaaatacga cttgtggcgc gccataacca cgcatcgcac cagccgaggg
     5101 gaggttggta tagcaggtct ttgaactgta agcgtaggca caacgaggat aaaggtaagc
     5161 gactttattc ccccccgcag aagcgatgga gtgcccgtga gatgcataag cgccggtgtt
     5221 agacagaaca tccagactat aacctttcaa tgttccgtcg cggttcacgc ccatttgccc
     5281 gtcaatggta aaagcgtggc gggtacgggt tgcgaggaaa cactcttcac ggctaaggga
     5341 aactttcacc ggaatgccgc caagcttgct ggtcaggaat gccgccattg gctcttccag
     5401 tacatcctgt ttattaccaa aaccgccacc gacaaatggt ttgatgactc gtacgcatga
     5461 ccagggaata tccagcgcct gaccaaccac gcggcgaaca atgtgcggga tctgggtgct
     5521 ggaaacgatg gtaattcgcg agtcatcctc catccacgcc agcgatgtta cgctttccat
     5581 gtgacaatgt tgaataacgg gggtctgata gtgcccctgt acctggtagt cggcggcatc
     5641 gattgtttgt tggacattac ccgtcgacat cgtgctttgt ttcagtaaat tgccaccgtt
     5701 atggattggt gcagcgtctt ctgccagcgc cgcttctggc gtggtgataa cgggtaattc
     5761 ttgccactca atgctgacca attgcgccgc tttttctgcc gtgagttcat cgcgggccac
     5821 gacgatggca atggcgtcac catgatgacg aacatggcga gttagcagtg cgcgatcggc
     5881 ggtatcgcgc ttgttttcgt caagtgtcca ggcatgccca gctgtagcga atggaatatc
     5941 aggcacatct tcccaggtaa aaatcgccag tacgcccggc aaacttctgg cttgttcatc
     6001 attaatactt acggcataac catgtgcgat agggctacgt acatatttcg cataacacat
     6061 gcccgccata acataatcgt cagtatatcg tgcccgcccg gtgaccttag caatggcatc
     6121 gacgcgcatg catgattcac ccgtagcggt tgcttcccgc gcttccatag aaatcccctc
     6181 gatttgaaca tttggttctt atggcaaaga tttagtctaa gcaggcaaga tttacgccag
     6241 aaacgacgat aagcgttagg ggaggtagga agtgtgatga cgttaaaaaa acacagatat
     6301 gatacgaaac aggagtctga ataggggcag tgatacgtat caatatgagt ggcgataaaa
     6361 tcaatgtgat aaattgggat tattacgcat cagg
//
LOCUS       DAJTNN010000199         6340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTNN010000199 DAJTNN010000000
VERSION     DAJTNN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.201"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..357
                     /locus_tag="OO150_003657"
     CDS             <1..357
                     /locus_tag="OO150_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517651.1"
                     /translation="AITALNFYQGDVPSVMIRIQPENMSPLIIDISTGEHDNYIIQTL
                     DVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDC
                     WQILFFSPDHFNNDFY"
     gene            379..1584
                     /locus_tag="OO150_003658"
     CDS             379..1584
                     /locus_tag="OO150_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517652.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPEIPIENNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVILYNDSFLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNTTFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNPFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPV
                     LISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDIIIEDYENIKENAYLVVPQINYTMD
                     LNIEDSSSEELIPSRKTKILRTMALKSKNITHMKHITQRIYILPERNIRMITTFQMQY
                     RLNNFLQNL"
     gene            complement(1944..2320)
                     /gene="rnpB"
                     /locus_tag="OO150_003659"
     ncRNA           complement(1944..2320)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO150_003659"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2353..3498)
                     /gene="garK"
                     /locus_tag="OO150_003660"
     CDS             complement(2353..3498)
                     /gene="garK"
                     /locus_tag="OO150_003660"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5517653.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTVL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3595..4485)
                     /gene="garR"
                     /locus_tag="OO150_003661"
     CDS             complement(3595..4485)
                     /gene="garR"
                     /locus_tag="OO150_003661"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5517654.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4515..5285)
                     /gene="garL"
                     /locus_tag="OO150_003662"
     CDS             complement(4515..5285)
                     /gene="garL"
                     /locus_tag="OO150_003662"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR5517655.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(5301..>6340)
                     /gene="garP"
                     /locus_tag="OO150_003663"
     CDS             complement(5301..>6340)
                     /gene="garP"
                     /locus_tag="OO150_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5517656.1"
                     /translation="QGFVDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPT
                     KERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIYN
                     PTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQ
                     YFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRG
                     LSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTA
                     PKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFV
                     VGDIKRMELQK"
BASE COUNT         1723 a         1661 c         1376 g         1580 t
ORIGIN      
        1 gcaattactg cattgaattt ctatcaaggc gatgttcctt ctgtcatgat ccgaatccaa
       61 ccggaaaata tgagtccatt aatcatagat atttctacag gagaacatga taattatatc
      121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
      181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
      241 acccaaaaag aagccatctt aacagcccct acgcacttta ataaaacctg cattacttca
      301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
      361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
      421 ttagtccgtc gaaaaatgaa tgaccttcct gaaatcccca tagaaaataa tctcgaagca
      481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
      541 gcgttacaac caggtggaaa atgtattatc actggtgatt ttcaaaaaga attagcgtac
      601 ttacaaaatg taattcttta taatgactcg tttcttcgtc tggatttttt aggttataac
      661 gcccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
      721 aaccaggaaa tatctacagg caatatcaat gttaattgtc cattaaaaga tatttacaat
      781 gaaatcagga ggttgaacac aacctttagt tgtgggactg gagatatagt tgatctatcc
      841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
      901 aatactattc taaatccttt taaattggat tcaacaaatt ttactaatgc caacatgttt
      961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
     1021 ataacgcctg ttttaataag tattagcgat atgtattctg aagaaaaaat caagtttgtc
     1081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
     1141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
     1201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
     1261 gagatgaaag aattctcatc aaaaaaaaca tatgattaca tcgatataat cattgaagat
     1321 tatgaaaata tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat
     1381 ttaaacatag aagactccag ctcagaagag ttaataccct cgagaaagac gaaaattctt
     1441 cggacaatgg ctttgaagtc gaagaatata acgcatatga agcatataac tcagagaatc
     1501 tatattttac cagagaggaa tatacgtatg attacgacct ttcaaatgca atatagactt
     1561 aataacttct tacaaaatct ataattaata ccgtagatat tcattcatct taccctgtgc
     1621 accaaaagca aaaactcact gacgcgggtt tgaaactgat ggcaaccgca aaaatgcctg
     1681 atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg cgtgcttttg
     1741 taggcaggat aaggcgttca cgccgcatcc ggcatgaata aagcgcactc aacaatctga
     1801 aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag ccgctttctt
     1861 ttgggtatag tgtcgtggac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc
     1921 cgaagcgggt ttttacgtaa atcaggtgaa actgaccgat aagccgggtt ctgtcgtgga
     1981 cagtcattca tctaggccag caatcgctca ctggctcaag cagcctaccc gggttcagta
     2041 cgggccgtac cttatgaacc cctatttggc cttgctccgg gtggagttta ccgtgccacg
     2101 gactgttacc agccgcgcgg tgcgctctta ccgcaccctt tcacccttac ctgatcccgc
     2161 ttgcgcgggc catcggcggt ttgctctctg ttgcactggt cgtgggtttc ccccccaggc
     2221 gttacctggc accctgccct atggagcccg gactttcctc ccctccgccc gtctcccccg
     2281 aagaggacga cgacgaagcg gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt
     2341 tgcgcgccct tgtcaccccg cgttgcgcat tccaatcgcc agtgtcgcgg cgatattacg
     2401 tgaagcacgg cagatattgt cataagcccc gcggaatgct tcgtccaacg tacctatgct
     2461 ggtcaatacg ctgaagaccg catcaatgcc atgctgatgt acaacgccaa catcatcggt
     2521 caggctaccc gcaatgccaa tcaccggttt atggtacttc ttcgccacgt ttgcgacacc
     2581 aatcggtacc ttgccgtgaa tactctggct gtcaatacgc ccttcaccgg tgatcaccag
     2641 cgtacaatcg tgaatatgtt cttccagatt cagcaccgta gtgacgattt caataccact
     2701 tttcagttcc gcaccaagaa acgccatcag cgccgcgccc ataccgcccg cagctcctgc
     2761 gccggggaca tctttcacat caacatgcag cgctttttta atgacctcgg catagtgaga
     2821 gaggttattg tccagctcaa caatcatcgc ttcactggct cccttttgtg ggccaaagat
     2881 gcgcgatgcg ccgttatcgc ccaccagcgg attggtgaca tcacaagcga cgcgaatgac
     2941 gcaatctttt aagcgcggat cgaggccgga aatatcaata tcattcagag tattaagact
     3001 accgccgcca aaaccaattt cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg
     3061 taccatgcct gcgccgccat catttgtagc gctgccgcca atgccgataa taatgtttgt
     3121 cgcaccgctc tccagcgcct gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag
     3181 tggatcgcgt ttttccgcag gtaccagctc cagcccactg gccgccgcca tttcaataaa
     3241 cgcggttttg ccatcgccgg agatccccca actggcattc actttctcgc ccaacggccc
     3301 tgtaacccag gcgtgacgtt cggaaccctg ggtggctgca atcatcgctt ccaccgttcc
     3361 ttcgccaccg tcggcaaccg gaacagaaac gtactgtgca tcaggaaaaa tttcccgaaa
     3421 tcctttttct atcgcctgcg caacctcgct ggcagataaa ctttctttat aagagtctgg
     3481 ggcgattacg attttcatac ctatgcctgt taccacatga cgccggaggg cgtttctctt
     3541 attcggcgtg gattccaggc ccggattgca atacgccatc cgggcacgac gtcattaacg
     3601 agtaacttcg actttcgcca gtttttcgta gtagcacgcc agagcgctat gatccgccgt
     3661 tcctaaacca tctgcacgca gtgcctgcat catctccata accgcagctg tgagcggcag
     3721 ttgcgcgccg acgccgtgag aagtatccag cgcattcgcc agatccttaa tatgcagatc
     3781 aatgcggaag cccggcttga agttgcggtc catcaccatc ggcgctttgg catccagcac
     3841 ggtactgccc gccagcccac cgcgaattgc ctgataaacc aggtccgggt taacgcccgc
     3901 tttagttgcc agcgttaacg cttctgacat cgcagcaata ttcagcgcca caatgacctg
     3961 atttgccagt ttggtgacgt tacctgcacc gatttccccg gtatgcacca cggaacccgc
     4021 catcgctttc atcaaatcat agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac
     4081 tgacagcgta ccgtcgatgg ctttcggttc accgccgctc accggagcat ccagcatatc
     4141 aatgcctttc gctttcagcg cttcgctgat ttcacggctt gccagcggtg cgatagaact
     4201 catatcgatc aataccgtac ctggcttcgc gccttcaata atgccattct cacccagcgc
     4261 cacctctttc acatgagggg agtttggcag catggttatg atgacgtcgc actgttcagc
     4321 gatcgcttta gccgtagacg ctgtttctgc acctgcagca atcacgtcag caatagcttc
     4381 tgggttacgg tcagcaacca ccagcgagta acctgctttc agaaggtttt tactcattgg
     4441 tttacccata atccccaggc caataaaacc aactttcata gtcatatcaa tcatctctct
     4501 tgttgcggtg gtggttattt tttaaaggta tcagccagtt tctgagtggc agagcggaag
     4561 acgccaagat cgctgccgac agccacaaac gtcgcgcccc attccagata acgacgcgca
     4621 tcggcttcga ccggcgcgag aataccgctg ggtttgccgt gcgcactggc acggttaaaa
     4681 atatgctgaa ttgctttttg tacatccgga tgcgaggcat tgccgagatg gcccaatgcc
     4741 gcagccagat cgctggggcc gacgaagata ccgtctacac cttcggtagc ggcaatagca
     4801 tcgacgttat ctacgccctg ctgactttct atctggacca gaatagtgat gttcttgttc
     4861 gactgagcga aataatccgc cacggtgcca aacatattgg cgcggtgaga aacggagacg
     4921 ccgcgaatgc cttccggcgg gtaacgagtt gatgccaccg cctgctctgc ttcctctttt
     4981 gtttctacaa aaggaatcag gaagttatag aaaccgatat ccagaagacg cttaataatt
     5041 accggctcgt tggtcggcac tcgcactact ggcgcgctgg cgctgccttt cagggccatt
     5101 aactgcggaa taaacgtgga aatatcgttt ggcgcatgtt cgccatccag caccagccag
     5161 tcaaacccag ccaaaccaag aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa
     5221 ccaatttgta cctgtttcgc agccagtgcg gctttgaatt tattcgggaa aacatcgtta
     5281 ttcatcgctt atacctttgc ttatttctgc aattccatac gtttaatgtc gccaactacg
     5341 aagaggtagc agaccatcgc catcagcgct gaacatccca cgaaaaccag tgctgcattg
     5401 aaggagtgca gttcacttac caggtagcca atcaccagtg gagtgacaat ggaggcaaca
     5461 ttgccaaaga cgttaaagac gccgccacag aggccaacaa tctctttcgg cgcggtgtca
     5521 gaaatcaccg gccagcccag agcaccaaat cctttgccaa aaaaagccag cgccatcagc
     5581 atgaccacca gcgtggtgtt gttggtgtag ttacataaga tgatggtgga agccagcaac
     5641 attcccagca caatcggtag cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga
     5701 taatccgaga agacacctcc cagcacgccg cccgcaaaac cacacagtgc cggaatcgag
     5761 gcgaccagac ccactttcag aatcgacatg cctttttcct gcaccagata aatcgggaac
     5821 caggtgagga agaaccaggt gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac
     5881 atcatgcggt tagagagcaa ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg
     5941 ctgcccggct ttttgtggtc catatcgacc accgcgccat tttcagagat aaacttcagc
     6001 tcttccgcag acatacgtgg gtgatctgtc gggttataaa tcaacttgat ccacagcgcc
     6061 gtcagcacaa aaccaatcac ccccataacg gtgaagacgt gctcccagcc ccaggcgaaa
     6121 gtcagccagc caagcagagg cgaaaagagc gccagcgaga aatattgcgc cgagttaaag
     6181 atggcggagg cagtaccacg ttctttcgtc gggaaccagg cggcgacaat acgggcgttc
     6241 gccgggaatg atggcgcttc cgagaagccg agcataaagc gcataaagaa catagagatc
     6301 cctgcccagg ccagcgggaa catatcaaca aagccttgca
//
LOCUS       DAJTNN010000200         6336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTNN010000200 DAJTNN010000000
VERSION     DAJTNN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.202"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            31..153
                     /locus_tag="OO150_003664"
     CDS             31..153
                     /locus_tag="OO150_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR5517657.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDT"
     gene            169..1014
                     /locus_tag="OO150_003665"
     CDS             169..1014
                     /locus_tag="OO150_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5517658.1"
                     /translation="MRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDA
                     IILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLSN
                     AAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDK
                     AISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELE
                     NNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATH
                     VDGLP"
     gene            1037..2548
                     /locus_tag="OO150_003666"
     CDS             1037..2548
                     /locus_tag="OO150_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5517659.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGGRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2573..3571
                     /locus_tag="OO150_003667"
     CDS             2573..3571
                     /locus_tag="OO150_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5517660.1"
                     /translation="MSASSLSLPLGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3604..4698
                     /locus_tag="OO150_003668"
     CDS             3604..4698
                     /locus_tag="OO150_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5517661.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4710..5582
                     /locus_tag="OO150_003669"
     CDS             4710..5582
                     /locus_tag="OO150_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5517662.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5630..6052)
                     /locus_tag="OO150_003670"
     CDS             complement(5630..6052)
                     /locus_tag="OO150_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5517663.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIILGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6149..>6336)
                     /locus_tag="OO150_003671"
     CDS             complement(6149..>6336)
                     /locus_tag="OO150_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenoxybenzoate dioxygenase"
                     /protein_id="HCR5517664.1"
                     /translation="LCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGK
                     TFDAKLLIDENIALKSL"
BASE COUNT         1466 a         1515 c         1836 g         1519 t
ORIGIN      
        1 gttgttaata aaaaccctac aggaggcact atgcctaaaa aaatgagaac aacccgtaat
       61 ctattattaa tggctacgct attaggtagc gcactatttg ccagggctgc ggataaagaa
      121 atgaccattg gtgcaatata ccttgatacc tagggatatt acgctggagt gcgtcagggc
      181 gttcaggatg cggcaaaaga ttcttcagta caggtacagt taattgaaac taacgcccag
      241 ggtgatattt cgaaagaaag tacctttgtt gataccctcg tggcgcgtaa tgtcgatgcc
      301 attattttat cggcagtgtc tgaaaatggc agtagccgta ccgttcgtcg cgccagtgaa
      361 gcgggtattc cggtgatttg ctacaacacc tgtattaatc aaaagggtgt cgataaatat
      421 gtctcggcgt atctggtcgg cgatccactg gaatttggta aaaaactgag taacgctgcc
      481 gccgattatt ttattgccaa taaaattgac cagccgaaaa ttgccgtcat caattgcgaa
      541 gcctttgaag tttgtgtgca gcgacgtaaa ggatttgaag aagtattaaa atcccgcgtt
      601 cccggcgcgc aaattgtcgc taatcaggaa gggactgttt tagataaagc gatttccgtt
      661 ggtgaaaaac tgattatctc cacgccggat ctcaacgcca ttatggggga gtcgggcggt
      721 gcgacactcg gcgcggtaaa agcggtacgt aatcaaaatc aggccggaaa aattgctgtt
      781 ttcggttcgg atatgacaac cgaaattgct caggagctgg aaaacaatca ggtgctgaaa
      841 gcggtagtgg atatttccgg taagaaaatg ggcaatgctg ttttcgcgca aacattgaag
      901 gttatcaata aacaagccga cggtgaaaaa gtgattcagg tgccaatcga tctctatacc
      961 aaaacggaag atggtaaaca gtggctggca acgcacgttg atggtctgcc ctaatcgctg
     1021 attcgcagag gtccctatgt tcacggcaac agaggcagtc ccggtagcaa aagtggtggc
     1081 aggaaataag cgttaccccg gcgtcgttgc gctggataac gttaacttca cgctcaataa
     1141 aggcgaagtt cgtgcgctgt taggtaaaaa cggcgcgggc aaatcgactc tcattcgaat
     1201 gcttaccggc agcgaacgcc cggatagcgg tgatatctgg attggcgaga cgcgactgga
     1261 aggtgacgaa gctacgttga ctcgccgtgc cgctgaactg gggggtcgtg cggtttatca
     1321 ggaattaagt ctggtggaag ggctgacggt ggcagaaaac ctctgccttg gtcaatggcc
     1381 tcgccgcaac ggcatgattg attacctgca aatggcgcag gatgcccaac gttgcttaca
     1441 ggcgctgggc gttgacgtta gtcctgaaca acttgtttca acgctaagcc cggcgcaaaa
     1501 gcaactggtg gaaattgcac gggtgatgaa gggcgagccg cgcgtggtca ttcttgatga
     1561 acccaccagc tcgcttgcca gtgcagaagt tgaactggtg atcagtgcgg tgaaaaagat
     1621 gtcggcactg ggcgtggcgg tgatttatgt cagccaccgg atggaagaaa tccgccgcat
     1681 tgcctcctgt gccaccgtta tgcgcgatgg tcaggtggcg ggcgatgtga tgctcgaaaa
     1741 cacctccacg catcatattg tgtcgctaat gctagggcgt gatcacgttg atattgcgcc
     1801 ggttgcccct caggaaattg tggatcaggc cgtactggaa gtccgtgcgt tacgccataa
     1861 gcccaagctg gaggatatca gctttacgtt acgtcgcggc gaagtgcttg gcattgctgg
     1921 cctgctgggg gcagggcgca gtgaattgct gaaggcgatt gttgggctgg agacgtatga
     1981 acagggcgaa attgttatca acggcgagaa aatcacgcgc cccgattacg gcgacatgct
     2041 gaaacgcggc attggctata cgccagaaaa ccgcaaagaa gcggggatca ttccctggtt
     2101 gggcgttgac gaaaatacag tgctgaccaa tcggcaaaaa atcagcgcca acggagtgct
     2161 gcaatggtcc accatccgcc gcctgaccga agaggtgatg cagcggatga cggtcaaggc
     2221 cgccagtagc gaaacgccca tcggcacgct ttctggtggc aatcagcaaa aagtggtgat
     2281 cggtcgttgg gtctatgccg ccagccagat tttgttgctc gacgagccaa cgcgcggcgt
     2341 cgatatcgaa gccaaacagc agatttaccg tattgtccgt gagctggctg ccgaaggaaa
     2401 aagcgtggtg tttatctcca gtgaagtgga ggagttgccg ctggtgtgcg accgcatcct
     2461 gttgttacag cacggtacgt tctcgcagga gtttcactcg ccggtcaatg tggatgagct
     2521 gatgtccgcc attctgtctg tgcactgatg aattttaccg aggaacctga agatgtctgc
     2581 ttcgtcatta tcattgccgc tgggcaagag cgtctcgctc aaacaatttg tcagtcgcca
     2641 tattaatgag atcggtttgc tggtggtaat cgccattctt tatctggtct tctccctgaa
     2701 tgcgcctggc tttatctcat tgaataacca gatgaacgtg cttcgcgatg ctgccaccat
     2761 cgggatcgcc gcctgggcaa tgacgctgat tattatctcc ggtgaaattg atgtcagcgt
     2821 tggaccgatg gtggcttttg tctcggtgtg cctggcattt ttgctgcaat ttgaagttcc
     2881 gttggcgatt gcttgtctgc tggtgttgct gttaggcgcg ctgatgggaa cgctcgccgg
     2941 ggtgctgcgc ggcgtgttta acgtgcccag tttcgttgcc acactggggc tgtggagcgc
     3001 cctgcgcgga atggggctgt ttatgaccaa cgcactgcca gtgccgattg acgaaaacga
     3061 ggtgctggac tggctgggcg gacaatttct cggtgtgcca gtatccgcgc tgatcatgat
     3121 ggtgttgttt gcgctgtttg tgttcattag ccgaaaaacc gccttcgggc gctcggtttt
     3181 tgccgttggc ggtaatgcca cggcggcgca gttgtgcggt attaacgttc gtcgagtgcg
     3241 cattcttatc tttacccttt cgggattatt agcggcggtg accggcattt tgctggcggc
     3301 acgcctcggt tccggtaacg caggtgccgc aaacggtctg gagtttgacg tcatcgccgc
     3361 ggtcgtggtc ggcggtacgg cactttccgg cggtcgcggt tccctgttcg gtacattgct
     3421 tggtgtgctg gtgattacgc taatcggtaa cggtctggtg ctgctcggta ttaactcctt
     3481 tttccagcag gtggtacgcg gcgtcatcat cgtggtggcg gtgctggcga atatcttgct
     3541 gacccagcga agcagtaaag cgaaacgcta aaccttaacg cccctggcgc gggtgcggca
     3601 gggatgaaaa ttaatcgtat tgttaatgag ggatttatga aaacgatgct ggcagcttat
     3661 ttaccaggaa attcaaccgt cgatctgcgg gaagttgcgg tgccgacgcc ggggattaac
     3721 caggtactga tcaaaatgaa atcctccggg atttgcggaa gcgatgtcca ctatatctat
     3781 catcaacacc gtgccacggc ggcggcaccc gataaaccgt tataccaggg atttatcaac
     3841 ggtcatgaac catgcgggca gattgtggcg atggggcaag gctgtcgcca ttttaaagag
     3901 ggtgatcgcg tgctggtgta tcacatttct ggctgtggtt tttgcccgaa ctgccgccgc
     3961 ggctttccta tctcttgtac tggcaaagga aaagcggctt acggctggca gcgtgacggc
     4021 ggtcatgccg aatacttgct ggcggaagaa aaagatctga tcctcctgcc ggatgcgctt
     4081 agctacgaag atggtgcgtt tatcagttgc ggcgttggta cagcgtatga aggaattttg
     4141 cgcggcgaag tttccggcag tgataacgtg ctggtggtcg gtctggggcc agtcggcatg
     4201 atggcgatga tgctggcgaa aggtcgcggt gcaaaacgga tcatcggcgt tgatatgctg
     4261 ccggaacgtc tggcgatggc aaaacagtta ggggtgatgg atcacggcta tttagcaacc
     4321 accgaaggtc tgccgcagat tatcgccgaa ctcacccacg gtggcgcgga tgttgcgctc
     4381 gattgttctg gtaatgccgc aggtcgcttg ctggcactgc gatccaccgc tgattgggga
     4441 cgggtggttt acatcggaga aaccggaaaa gtggagttcg aggtcagcgc cgatctgatg
     4501 caccatcaac ggcggattat cggctcctgg gtgaccagtc tgttccatat ggaaaaatgc
     4561 gcccatgatc tgacggactg gaaactgtgg ccgcgtaacg ccattaccca tcgcttctcg
     4621 ctggaacagg ctggagatgc ctatgcgctg atggcgagcg gcaaatgcgg gaaagttgtg
     4681 attaacttcc cggattaagg agttagcgga tgaccatcta taccttatct cacggaccgc
     4741 taaagctgga tgtctctgac cagggcggcg taatcgaagg cttctggcgt gacacgacgc
     4801 cgcttttacg tcctggtaaa aaaagcggtg tggcaaccga tgcttcctgt tttccgctgg
     4861 tgccgtttgc aaaccgggtg agcggcaatc gttttgtctg gcaggggcgt gagtatcaac
     4921 tgcaaccgaa tgttgagtgg gatgcgcact atctacacgg cgatggctgg ctgggcgagt
     4981 ggcaatgtgt ctcgcacagc gatgatagct tgtgtctggt gtatgaacat cgcagcggtg
     5041 tctatcacta tcgggtaagt caggcgtttc atttaacggc ggatacgctg acggtgacgc
     5101 tctctgtcac caatcaaggg gcagagacgc tgccatttgg taccggctgg catccttatt
     5161 ttccgttgtc accacaaacg cggattcagg cgcaggcgag cggttactgg ctggagcggg
     5221 agcagtggct ggcgggtgag ttttgcgagc agctaccgca ggaactggat tttaaccagc
     5281 tcgcgccgtt gccgcgccag tgggtgaaca atggttttgc cggatggaat ggtcaggccc
     5341 gcatcgagca gccgcaggaa ggttatgcca tcatcatgga aacgacgcca cctgcaccgt
     5401 gttatttcat ctttgtttcc gaccctgcgt ttgataaagg atatgcgttt gatttcttct
     5461 gtctggaacc gatgagccat gcgccggatg atcatcatcg cccggaaggg ggtgatctca
     5521 ttgcgctagc accaggggaa tcaacaactt cagagatgtc gttgcgggta gagtggctgt
     5581 aatttattaa atgccggata aggcgttcac gccgcatccg gcatctttcc taacgccgat
     5641 cgagagaaat tgcccccggc ccggtaattg ccaatagcaa gaacgcgcca gcgatactga
     5701 cattcttcca gaaattaatc atatttggcc caaccgcatc gccggtcata tcccagtaat
     5761 ggtgaccaat caccgccgta cccagcgtgt agaaaataaa cagcaccgcc agcggacggg
     5821 tgaaaaagcc aagcacgatt aatatcgcgg cgggcacttc cataactacc gcaataatcg
     5881 ctgccagcat cggcattggc gcgcccaacg aggccatata ttggaccgta ccgtcaaagc
     5941 ccatcatttt gggaaaaccc aaaataatga aaattaagac cacggcgata cgggcaatta
     6001 ataacaaaac ggggcgggca gctccaaaat caaaataacg tagtgtgttc ataaccaatc
     6061 cctttgcaag ataatgtgat ttaaacgtaa tacacactct tgcacctcgc cacgccccag
     6121 gcgttgagat attcgctaat tatcctggtt acagtgattt aagcgcgatg ttctcatcta
     6181 tcagcagttt cgcatcaaac gttttgccgc tctggatcca tttgcgaatt gggcgaatct
     6241 cacgcccctg attcagcgtg accgcaccga taagcacgcc gttttgcaga ttaaaccaaa
     6301 tcgccttctg agtttccggg ttgccacgac aaagcc
//
LOCUS       DAJTNN010000201         6330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTNN010000201 DAJTNN010000000
VERSION     DAJTNN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.203"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..649
                     /locus_tag="OO150_003672"
     CDS             <1..649
                     /locus_tag="OO150_003672"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5517665.1"
                     /translation="TYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
                     QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGH
                     AAFVDAGKLLPNIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAE
                     LLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            740..1987
                     /gene="tnaB"
                     /locus_tag="OO150_003673"
     CDS             740..1987
                     /gene="tnaB"
                     /locus_tag="OO150_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5517666.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2119..3294
                     /gene="mdtL"
                     /locus_tag="OO150_003674"
     CDS             2119..3294
                     /gene="mdtL"
                     /locus_tag="OO150_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5517667.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3269..4228
                     /gene="yidZ"
                     /locus_tag="OO150_003675"
     CDS             3269..4228
                     /gene="yidZ"
                     /locus_tag="OO150_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5517668.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4385..5134
                     /locus_tag="OO150_003676"
     CDS             4385..5134
                     /locus_tag="OO150_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5517669.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5156..5722
                     /gene="yieF"
                     /locus_tag="OO150_003677"
                     /gene_synonym="chrR"
     CDS             5156..5722
                     /gene="yieF"
                     /locus_tag="OO150_003677"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5517670.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5776..>6330)
                     /gene="adeP"
                     /locus_tag="OO150_003678"
     CDS             complement(5776..>6330)
                     /gene="adeP"
                     /locus_tag="OO150_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5517671.1"
                     /translation="LVNLFDSSGTLIGVTDKAGLADEKGKFPRMKQALYVDSISSVTG
                     SFIGTSSVTAYIESSSGVSVGGRTGLTAVVVGLLFLLVIFLSPLAGMVPGYAAAGALI
                     YVGVLMTSSLARVNWQDLTESVPAFITAVMMPFSFSITEGIALGFISYCVMKIGTGRL
                     RDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1463 a         1594 c         1614 g         1659 t
ORIGIN      
        1 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat
       61 gggcggcttg ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac
      121 cctttgcgtg gtgcaggaag gcttcccgac atatggcggc ctggaaggcg gcgcgatgga
      181 gcgtctggcg gtaggtctgt atgacggcat gaatctcgac tggctggctt atcgtatcgc
      241 gcaggtacag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
      301 cggtcacgcg gcattcgttg atgccggtaa actgctgccg aatatcccgg cagaccagtt
      361 cccggcacag gcgctggcgt gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat
      421 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga
      481 actgctgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
      541 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttaccta
      601 cgaaccaaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca
      661 gagtggctat aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc
      721 ttcctctaaa ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg
      781 tgttatggtt atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct
      841 tgccggtgcc tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct
      901 tcattccggg ttattgttat tagaagcaaa tttaaattat cctgtcggct ccagttttaa
      961 caccatcacc aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc
     1021 cttcgttctc tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga
     1081 aacgatatca atgaatttgg gctatcacgc taatccacgt attgtcggga tctgcacagc
     1141 cattttcgtt gccagcgtat tgtggataag ttcgttagcc gccagtcgca ttacctcatt
     1201 gttcctcggg ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt
     1261 cgattactcc atcctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta
     1321 tatctttatg gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag
     1381 cctgattatt tgctatggaa aacgcaaaga taagttaatc aaaagcgtgg tatttggttc
     1441 gctgctggcg ctggtgattt atctcttctg gctctattgc acgatgggga atattccgcg
     1501 cgaaagcttt aaggcgataa tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt
     1561 cctcggcacc aaacagcacg gcattatcga gttttgcctg ctggtgttct ctaacttagc
     1621 tgttgccagt tcgttctttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt
     1681 taagattgat aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc
     1741 tgcgttgttg tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccgggct
     1801 gtgcgccacc atctgggcgg tcattattcc cgcagtgctt gcaatcaaag ctcgcaagaa
     1861 gtttcccaat cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct
     1921 ctttggtata accgtgattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt
     1981 tggctaaatc cttcaagaag ccagccattc gctggcttct tgcctctcag gaaatcactt
     2041 atgtccaaat ggcaactcgc ctgatcctcc ttcaccacgt atgctttgcg tcaccttact
     2101 atcaggacgc tttagcccat gtcccgcttt ttgatttgta gttttgccct ggttttactt
     2161 tatcccgccg ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat
     2221 gccagcgaag cgcagttgca tattgcgttc tccgtatatc tggcggggat ggcagctgcg
     2281 atgttatttg ccggtaaagt ggccgatcgt tcagggagaa agccggtcgc catacccggc
     2341 gcggcgctat ttattattgc ctcggtgttc tgttcactgg ctgaaaccag cacgttattt
     2401 cttgcaggcc gatttctaca ggggttgggc gcaggctgtt gttacgtagt ggcgttcgcc
     2461 attttgcgcg acacgctgga tgatcgacgt cgggctaaag tgctgtcatt actcaacggt
     2521 attacctgca tcattccggt gttagcgcca gtgctcggac atctgattat gcttaaattc
     2581 ccgtggcaga gtctgttctg ggcgatggca atgatgggca tcgcggtact gatgttgtct
     2641 ttgtttattt taaaagaaac gcgcccagcg tcccccgctg cttcggacaa accacgagaa
     2701 aatagcgagt cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc
     2761 gtttcggtga tcctcacttt cgtcaataca tcgccggtat tgctgatgga aatcatgggt
     2821 tttgagcgcg gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt
     2881 tcattctcca cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg
     2941 caggtgttat tccttgcagc ggggatcacc cttgccgttt caccttccca tgcggtttct
     3001 ctgtttggta tcacactgat ttgcgccggt ttctcggtag gttttggcgt agcgatgagt
     3061 caggcgttag gaccattttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg
     3121 caggtttgcg gttcgtcact gtggatttgg ctggcagcgg tggttggtat cggcgcatgg
     3181 aatatgctga tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc
     3241 gtcgcgcctg gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa
     3301 tctgctgctc tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg
     3361 gatgaacgtg accccttcag cggtgagtaa gtcgctggca aagttaagag cgtggtttga
     3421 cgacccgctc tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat
     3481 ggagcaaaat ctggcggaat ggatgcaaat gagcaatctg ctgctggata aaccgcacca
     3541 ccagacaccg cgcggcctga agtttgagtt ggcggcggaa tcgccgctga tgatgatcat
     3601 gcttaatgcg ctgtcgaaac ggatctacca acgttacccg caggcgacca tcaaactacg
     3661 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc
     3721 cggtcgcgaa agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga
     3781 ttatgaagtg ctgtttagtg atgtaccctg cgtctggtta cgcaaagatc atccggcact
     3841 gcatgaaacg tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga
     3901 acagagcgac acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat
     3961 tgctatgagc ctgccggaat ttgagcagtc actgtttatg gctgcgcaac ccgacaatct
     4021 gctactggcg accgcgccgc gctactgtca gtactacaat caactccatc aactgccgtt
     4081 ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag ttccttttac
     4141 cctgctgtgg cataaacgga acagtcataa tccgaagatc gtctggttac gggaaaccat
     4201 taaaaacctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc caatttcaca
     4261 ataaaatgta aaaaagttgt aataagcttg tctgaatcga acttttagcc gctttagtct
     4321 gtccatcatt ccagtaaatg attactctta tattcataat ggaccattaa gcatggagcg
     4381 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     4441 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt
     4501 tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     4561 gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agtgataaaa atttgccttc
     4621 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     4681 tggcctcgat atggaactac agcgtgcgac gcgcgggttt catagcccac acgcgcccga
     4741 taaccacacc ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga
     4801 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     4861 gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca
     4921 tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt
     4981 cacgcccgcg attgaaaagc tcagtgtctg ggagttagat ggcaaacaca gctggaaaag
     5041 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     5101 gctctctacc gtgaagtctt tttcgccaaa ttgatagaca accacaggag tcattatgtc
     5161 tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     5221 ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     5281 cattgccgac attcccctgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac
     5341 ggttgaagct ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga
     5401 atataactac tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     5461 ggatcaaccg ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg
     5521 cggcgcgcgc tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat
     5581 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatcggc aaaccggaga
     5641 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt
     5701 tattcagcga gttaagatct aaataaaaaa cccgccagta atcatgcatg gcgggttttt
     5761 aatgcgctat cgattttaat gagcgtcgat aaatacaatc ttcaggataa acagcagcgc
     5821 aacgatgatt acgcacgggc taaggtcacg cagacgcccg gtaccaatct tcatcacgca
     5881 gtaggagata aagcccagcg caataccttc ggtaatcgaa aagctgaacg gcatcatcac
     5941 ggcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact
     6001 tgaggtcatc aacacgccaa cgtagatcaa cgcgccagct gcagcgtagc ctggcaccat
     6061 tcccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     6121 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     6181 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt
     6241 catgcgcggg aatttcccct tctcatccgc cagacctgct ttatcggtca cgccaatcag
     6301 cgtaccggag gagtcaaaca agttgaccaa
//
LOCUS       DAJTNN010000202         6308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTNN010000202 DAJTNN010000000
VERSION     DAJTNN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.204"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            275..616
                     /gene="raiA"
                     /locus_tag="OO150_003679"
     CDS             275..616
                     /gene="raiA"
                     /locus_tag="OO150_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5517672.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    719..844
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            720..767
                     /gene="pheL"
                     /locus_tag="OO150_003680"
     CDS             720..767
                     /gene="pheL"
                     /locus_tag="OO150_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR5517673.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            867..2027
                     /gene="pheA"
                     /locus_tag="OO150_003681"
     CDS             867..2027
                     /gene="pheA"
                     /locus_tag="OO150_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5517674.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2070..3191)
                     /gene="tyrA"
                     /locus_tag="OO150_003682"
     CDS             complement(2070..3191)
                     /gene="tyrA"
                     /locus_tag="OO150_003682"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5517675.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLKQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3202..4272)
                     /gene="aroF"
                     /locus_tag="OO150_003683"
     CDS             complement(3202..4272)
                     /gene="aroF"
                     /locus_tag="OO150_003683"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5517676.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDLETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4480..4845
                     /locus_tag="OO150_003684"
     CDS             4480..4845
                     /locus_tag="OO150_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5517677.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4995..5513
                     /locus_tag="OO150_003685"
     CDS             4995..5513
                     /locus_tag="OO150_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5517678.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAKQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5503..>6308
                     /gene="dgcN"
                     /locus_tag="OO150_003686"
     CDS             5503..>6308
                     /gene="dgcN"
                     /locus_tag="OO150_003686"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCR5517679.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINT"
BASE COUNT         1712 a         1610 c         1417 g         1569 t
ORIGIN      
        1 agtcagttgc ttaactgact ggcattacag cccttgagct gccgtttttt tattctgtta
       61 gttgtgaaac tgaagcgatt tagtcactat cgatctcatc aaatatggct cgctttgaga
      121 tattcctcaa gtaaaaaaac acctcttcct gcgatttctc acaaaaaaga ttcgttgaca
      181 aaaagtgaca aaattatgag attcccatca cacattttga catcaggaac ggtatgctga
      241 attcaccaag acgggaagac aagaggtaaa atttatgaca atgaacatta ccagcaaaca
      301 aatggaaatt actccggcca tccgccaaca tgtcgcagac cgtctcgcca aactggaaaa
      361 atggcaaaca catctgatta atccacatat cattctgtcc aaagagccac aagggtttgt
      421 tgctgacgcc acaatcaata cacctaacgg cgttctggtt gccagtggta aacatgaaga
      481 tatgtacact gcaattaacg aattgatcaa caagctggaa cggcagctca ataaactgca
      541 gcacaaaggc gaagcacgtc gtgccgcaac atcggtgaaa gacgccaact tcgtcgaaga
      601 agttgaagaa gagtagtcct ttatattgag tgtatcgcca acgcgccttc gggcgcgttt
      661 tttgttgaca gcgtgaaaac agtacgggta ctgtactaaa gtcacttaag gaaacaaaca
      721 tgaaacacac accgtttttc ttcgcattct tttttacctt cccctgaatg ggaggcgttt
      781 cgtcgtgtga aacagaatgc gaagacgaac aataaggcct cccaaaccgg ggggcctttt
      841 ttattgataa caaaaaaggc aacactatga catcggaaaa tccattactg gcgctgcgag
      901 agaaaatcag cgcgctggat gaaaaattat tagcattact ggcagagcgg cgcgaactgg
      961 ccgtcgaggt gggaaaagcc aaactgctct cgcatcgccc ggtacgagat attgatcgtg
     1021 aacgcgattt actggaaaga ttaattacgc tcggtaaagc gcaccatctg gacgcccatt
     1081 acattactcg cctgttccag ctcatcattg aagattccgt attaactcag caggctttgc
     1141 tccagcaaca tctcaataaa attaatccgc actcagcacg catcgctttt ctcggcccca
     1201 aaggttctta ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt gagcaattca
     1261 ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca ggtggaaacc ggccaggccg
     1321 actatgccgt cgtaccgatt gaaaatacca gctccggtgc cataaacgac gtttacgatc
     1381 tgctgcaaca taccagcttg tcgattgttg gcgagatgac gttaactatc gaccattgtt
     1441 tgttggtctc cggcacgact gatttatcca ccattaatac ggtctacagc catccgcagc
     1501 cattccagca atgcagcaaa ttccttaatc gttatcctca ctggaagatt gaatataccg
     1561 aaagtacgtc tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt
     1621 tgggaagcga agctggcggc actttgtacg gtttgcaggt actggagcgt attgaagcga
     1681 atcagcaaca aaacttcacc cgatttgtgg tgttggcacg taaagccatt aacgtgtctg
     1741 accaggttcc ggcgaaaacg acgttgttaa tggcgaccgg gcaacaagcc ggtgcgctgg
     1801 ttgaagcgtt gctggtgctg cgcaaccaca atctgattat gacccgtctg gaatcacgcc
     1861 cgattcacgg taatccatgg gaagagatgt tttatctgga tattcaggcc aatcttgaat
     1921 cagcggaaat gcaaaaagca ttgaaagagt taggtgaaat tacccgttca atgaaggtat
     1981 tgggctgtta cccaagtgag aacgtagtgc ctgttgatcc aacctgatga aaaggtgccg
     2041 gatgatgtga atcatccggc actggattat tactggcggt tgtcattcgc ctgacgcaat
     2101 aacacgcggc tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg
     2161 cggaaactgt caataaacgc ctgcttatcg ccctgcttca gcaactcaat cgcctcgccg
     2221 aaacgcttat agtaacgttt gattaacgcc agattacgct ctgacgacat aataatgtcg
     2281 gcataaagct gcggatcctg agcaaacagt cgcccgacca tcgccagctc aaggcggtaa
     2341 atcggcgaag agagcgccag aagttgctca agctgaacat tttcttctgc cagatgcagc
     2401 ccataagcaa aagtagcaaa gtggcgcaga gcctgaataa acgccatatt ctgatcgtgc
     2461 tcgacagcgc taatacgatg cagccgagcg ccccagacct gaatttgctc cagaaaccat
     2521 tggtatgctt ccggcttacg tccatcacac cagaccacaa cttgctttgc caggctaccg
     2581 ctgtccgggc cgaacatcgg gtgtaacccc agtaccgggc catcgtgcgc cgccagcatg
     2641 gcctgtaatg gtccattttt cactgatgcc agatcaacca gaatacaatc tttcggtaaa
     2701 ggcggtaatt tgccgataac ttgctcagta acgtggattg gcacactaac aatcaccatt
     2761 ccggcatcgg caacaatatc agccgctcga tcccagtcat gttgctccag aatccgcacc
     2821 tgataacccg agagtgtcag catcttctcg aacaggcgtc ccatctgacc gccaccgccg
     2881 acgataacca ccggacgcag tgacggacaa agtgttttaa atcctttgtc gttttcactg
     2941 gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg cggtaccccc
     3001 agagcttccg cctctgcacg acgcgaggcc aacatagatg cctcgcgctc cggaacataa
     3061 ataggcagtc caaagcggct tttcacctcg cccacttcag caaccagttc cagacgcttc
     3121 gctaataaat tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa tgcggtcaat
     3181 tcagcaacca taataaacct cttaagccac gcgagccgtc agctgcccgt tcagatcctg
     3241 atgaatttca cgcagcaagg catcggtcat ttcccagcta atgcaggcat cggttacgga
     3301 tacaccgtat ttcatttcac tgcgcggttg ctcagaagac tgattgccct cgtggatatt
     3361 actttcgatc atcagaccaa taattgagcg attgccatct ttgatttgag caaccacgga
     3421 ttctgccacc gcaggctgac ggcgataatc tttattggaa ttaccgtggc tgcaatctac
     3481 catcagagac gggcgcagtc ccgcctgttc catctctttt tcacattgcg caacatccgc
     3541 agggctatag ttcggcgctt taccaccgcg caggatgaca tgcccgtccg gattcccctg
     3601 agtttgtagc aacgcaacct gccctgcctg gttaatgcca acaaaacggt gcggctgggc
     3661 ggcggcgcgc atagcgttaa ttgctgttgc cagactgccg tcggtgccgt ttttaaaacc
     3721 aaccggcatg gaaagcccgg aggccatttc acggtgagtt tgcgattccg ttgtacgagc
     3781 accaattgct gaccagctaa acagatcgcc caggtattgc gggctattcg gatctaacgc
     3841 ttccgtcgcc agtggcagtc ccatattcac cagctcaagc agcaatttac gcgcgatctg
     3901 cagcccggct tctacatcaa aagagccatc catatgggga tcgttaatta accctttcca
     3961 gccgacagtg gtacggggtt tttcaaaata gacgcgcatt accagataga ggctatcgct
     4021 gacctctgcg gcaagggctt taaatcgacg agcatattcc agagcagttt ccagatcatg
     4081 aatggaacaa ggaccacaaa ctaccagcag acgaggatcg cgcccggcga taatatctga
     4141 aatggtttta cgcgagtcag caatctgggc ttcttgttgc aggctcaatg gaaaagcggc
     4201 cttcagttgt tccggagtca ttaaaacctg ttcgtcggta atatgtacgt tattcagcgc
     4261 gtctttttgc atgatggcga tcctgtttat gctcgtttgc gatagttgat cctcagcgag
     4321 gatgacgtaa cgataacaca taaagtaaag ttttcaatcc atatttcgta cgcttttctt
     4381 tacacgaaca atttactcgc gctttgatgc ttatttcgta caaataaatt tacaccccct
     4441 ttaaaaacaa tccgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa gtttatcgcc
     4501 cccttattgg ctttactggt tagcggatgt cagattgatc cttatactca cgcgccaacc
     4561 ttgaccagca ccgactggta tgatgtcggt atggaagatg cgatatcggg cagcgccata
     4621 aaagatgacg atgcatttgg cgattcacag gcggatcgcg gtctatacct taaaggatat
     4681 gccgaaggac aaaagaaaac ttgccagacc gattttactt atgcccgggg actttccggt
     4741 aaaagctttc ctgccagctg taacaatgtt gaaagtgcca gccaactgca tgaagtatgg
     4801 caaaaagggg ctgatgaaaa tgccagcaca atacgattaa attaattaaa accccacaaa
     4861 taaattaaac ataagatttt actcattgtg ttttatttct cacattgatg acgatcgcat
     4921 gaaaaaatga tattttaatg agcattctga aaaaatatcg atgcatttcg agcgaagaag
     4981 gtggggatcc ctgaatgcgt ttttctcacc gactgttctt acttctgatt ctcctgctga
     5041 cgggagcacc aatactcgct caggagcctt ctgacgttgc gaaaaacgtg cgtatgatgg
     5101 tttccggtat tgtcagttac acccgctggc ctgcgctatc agggccacca aagttatgta
     5161 tcttttcatc ttcacgtttt agtaccgccc tacaagagaa tgccgcgaca tctttgcctt
     5221 atctgcctgt catcattcac actcaacaag aggcgatgat ttcaggctgc aatggttttt
     5281 attttggcaa cgagtcaccg acattccaga tggaattaac cgaacaatat ccgtcaaagg
     5341 cgttattatt aattgccaaa caaaataccg agtgcattat tggtagcgcc ttttgtctga
     5401 tcatccacaa taatgacgtc agatttgccg taaacctgga tgccttatcg cgtagcggtg
     5461 taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa aaatgatgga taacgataat
     5521 tctcttaata agcgccccac gtttaaaaga gcattacgca acatcagtat gaccagcata
     5581 tttatcacta tgatgctgat ctggttgctg ctttccgtga cctcggtgct gaccctgaaa
     5641 cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa tgacttacag tctggaagca
     5701 gctgtcgttt ttgccgatgg ccctgcagca actgaaacac tggcagcgct gggccagcaa
     5761 gggcaatttt caactgcaga agtacgtgat aagcagcaaa atattctggc gtcctggcat
     5821 tacacccgta aggatccagg cgatactttc agcaatttca taagccactg gctcttcccc
     5881 gcccccatca ttcagccgat tcgtcacaat ggtgaaacca ttggcgaagt acgcttaacc
     5941 gctcgcgaca gttcaatcag ccattttatc tggttttcgc tcgccgtact gaccggttgt
     6001 attctgctgg catcaggcat cgcaattacc ctcacccgcc atttgcacaa tggcctagtg
     6061 gaagcgctga aaaatatcac cgatgtcgta catgatgtgc gttccaaccg caatttttcc
     6121 cgacgagttt ctgaagaacg tatcgctgag tttcaccgct tcgctctcga cttcaacagt
     6181 ctgctggatg aaatggaaga gtggcagctt cgtttacagg ccaaaaatgc gcagcttctg
     6241 cgtaccgcgc tacatgaccc attaaccggg ctggctaacc gcgcagcgtt tcgtagcggt
     6301 atcaatac
//
LOCUS       DAJTNN010000203         6294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTNN010000203 DAJTNN010000000
VERSION     DAJTNN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.205"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(86..622)
                     /gene="ssb1"
                     /locus_tag="OO150_003687"
     CDS             complement(86..622)
                     /gene="ssb1"
                     /locus_tag="OO150_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5517680.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            876..3698
                     /gene="uvrA"
                     /locus_tag="OO150_003688"
     CDS             876..3698
                     /gene="uvrA"
                     /locus_tag="OO150_003688"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5517681.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3733..4089)
                     /locus_tag="OO150_003689"
     CDS             complement(3733..4089)
                     /locus_tag="OO150_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5517682.1"
                     /translation="MTISELLQYCMAKLGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLRPEEKRKLLVQL"
     gene            complement(4093..4509)
                     /locus_tag="OO150_003690"
     CDS             complement(4093..4509)
                     /locus_tag="OO150_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5517683.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLANMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4620..5333)
                     /gene="aphA"
                     /locus_tag="OO150_003691"
     CDS             complement(4620..5333)
                     /gene="aphA"
                     /locus_tag="OO150_003691"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5517684.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5735..5957
                     /locus_tag="OO150_003692"
                     /pseudo
     CDS             5735..5957
                     /locus_tag="OO150_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1593 a         1556 c         1568 g         1577 t
ORIGIN      
        1 ccataacgga ggcttctttt ttatggctgg cagatgcttt aatcatccac cttaaaacaa
       61 tataacctat tgttttaatg acaaatcaga acggaatgtc atcatcaaag tccatcggcg
      121 gctcgttaga cggcgctgcc ggagcggact gctgcgggcg agactgcgcg ccgccgctga
      181 actgattgcc accctgcggc tgctgaggct gaccccaacc gccctgcggc tgaccaccac
      241 cgatattacc acctgccgga gcgccaccac cctgacgacc acccagcatc tgcatggtgc
      301 cgccaacgtt caccacgact tctgtggtgt agcgatcctg accggattga tcggtccatt
      361 tacgggtacg cagctgacct tcgatataaa cctgagaacc tttacgcaga tattcgctgg
      421 ccacttctgc cagtttgccg aacagcacaa cgcggtgcca ttcagtctgt tctttcatct
      481 cgccggtcgc tttatcacgc caggattcgg aagtagccag cgtaatgttg gcaactgcgc
      541 caccatttgg catgtagcgt acttccgggt cctgacccag attaccaacg agaataacct
      601 tgtttacgcc tctgctggcc atgttcgtgt ctcctgaaaa aaatcgttct gaataagtgt
      661 aaacgcgcga ttgtaccatt accaatagcg cttttactat gttgtgacct cggttccggg
      721 aaacaaacct ggccagacat tgttacacaa cactccggat aatgcattcc aatactgtat
      781 attcattcag gtcaatttgt gtcataatta accgtctgtg atcgccggta gcaccatgcc
      841 accgggcaaa aaagcgttta atccgggaaa ggtgaatgga taagatcgaa gttcggggcg
      901 cccgcaccca taatctcaaa aacatcaacc tcgttatccc ccgcgacaag ctcattgtcg
      961 tgaccgggct ttcgggttct ggcaaatcct cgctcgcttt cgacacctta tatgccgaag
     1021 ggcagcgccg ttacgttgaa tccctttccg cctacgcgcg gcagtttctg tcactgatgg
     1081 aaaagccgga cgtcgatcat attgaggggc tttctcctgc catctcaatt gagcagaaat
     1141 cgacgtctca taacccgcgt tctacggtgg ggacaatcac cgaaatccac gactatttgc
     1201 gtttgttgtt cgcccgcgtc ggcgaaccgc gctgtccgga ccacgacgtc ccgctggcgg
     1261 cgcaaaccgt cagccagatg gtggataacg tgctgtcgca gccggaaggc aagcgtctga
     1321 tgctactcgc gccaatcatt aaagaacgca aaggcgaaca caccaaaacg ctggagaacc
     1381 tggcaagcca gggttacatc cgtgctcgta ttgatggcga agtctgcgat ctttccgatc
     1441 cgccgaaact ggaactgcaa aagaaacaca ctattgaagt ggtggttgat cgcttcaagg
     1501 tgcgtgacga tcttacccaa cgtcttgcgg agtcgtttga aaccgcgctg gagctttccg
     1561 gtggtacagc ggtagtggcg gatatggacg acccgaaagc ggaagagctg ctgttctccg
     1621 ccaacttcgc ctgcccaatt tgcggctaca gtatgcgtga actggagcca cgactgtttt
     1681 cgtttaacaa cccggcaggt gcctgcccaa cctgtgacgg ccttggcgta cagcaatatt
     1741 tcgatcctga ccgcgtgatc caaaaccccg agctgtcact ggctggcggt gcgatccgtg
     1801 gctgggatcg ccgcaacttc tattacttcc agatgctgaa atcgctggca gatcactata
     1861 agttcgacgt cgaagcgccg tggggcagcc tgagcgcgaa cgtgcataaa gtggtgttgt
     1921 acggttctgg caaagaaaac attgaattca aatacatgaa cgatcgtggc gatacctcca
     1981 tccgtcgtca tccgttcgaa ggcgtgctgc acaatatgga gcgccgttat aaagagacag
     2041 aatccagtgc ggtacgtgaa gaattagcca agtttatcag caatcgccca tgcgccagct
     2101 gcgaagggac gcgtctgcgt cgggaagcgc gccacgttta tgtcgagaat acgccgctgc
     2161 ctgctatctc cgacatgagc atcggtcatg cgatggaatt cttcaacaat ctcaaactag
     2221 ctggtcagcg ggcgaagatt gcagaaaaaa tccttaaaga gatcggcgat cgtctgaaat
     2281 tcctcgttaa cgtcggcctg aattacctga cactttcccg ctcggcagaa acactttccg
     2341 gcggtgaagc ccagcgtatc cgtctggcga gccagattgg tgcgggcctg gttggcgtta
     2401 tgtatgtact ggacgagccg tctatcggcc tgcaccagcg cgataacgag cgcctgttgg
     2461 gtacgcttat ccatctgcgc gatctcggta ataccgtgat tgtggtggag catgacgaag
     2521 acgcaattcg cgccgctgac catgtgatcg atatcggtcc tggtgcgggt gttcacggcg
     2581 gtgaagtggt cgcagaaggt ccgctggaag cgatcatggc ggtgcctgaa tcgttgaccg
     2641 ggcagtacat gagcggtaaa cgcaagattg aagtgccgaa gaaacgcgtt ccggcgaatc
     2701 cagaaaaagt gctgaagctg acaggtgcac gcggcaacaa cctgaaggac gtgacgctca
     2761 cgctgccagt cggtctgttt acctgcatca caggggtttc aggttccggt aaatcgacgc
     2821 tgattaacga cacactgttc ccgattgccc aacgccagtt gaatggtgcg accatcgccg
     2881 aaccggcacc gtatcgcgat attcaggggc tggagcattt cgataaagtg atcgatatcg
     2941 accaaagccc aattggtcgt actccacgtt ctaacccggc gacctatacc ggcgtgttta
     3001 cacctgtgcg cgaactgttt gcgggcgtac cggaatcccg tgcgcgtggt tatacgccag
     3061 gacgtttcag ctttaacgtc cgtggcgggc gctgcgaagc ctgtcagggc gacggtgtga
     3121 tcaaagtgga gatgcacttc ctgccggata tctacgtgcc gtgcgatcag tgcaaaggta
     3181 aacgctataa ccgtgaaacg ctggaaatta agtacaaagg caaaaccatc cacgaagtgc
     3241 tggatatgac catcgaagag gcgcgtgagt tctttgatgc ggtgccagct ctggcgcgta
     3301 agctgcaaac gttgatggac gttggtctga cgtacattcg cctggggcag tccgcaacca
     3361 cactttctgg tggtgaagcc cagcgcgtga agctggcgcg tgagctgtca aaacgcggca
     3421 ccgggcagac gctgtatatt ctcgacgagc cgaccaccgg tctgcacttc gccgatattc
     3481 agcaactcct cgacgtgctg cataaactgc gcgatcaggg caataccatt gtggtgattg
     3541 agcacaatct cgacgtgatc aaaaccgctg actggattgt cgacctggga ccagaaggcg
     3601 gcagtggcgg cggcgaaatc ctcgtctccg gtacgccaga aaccgtcgcg gagtgcgaag
     3661 cttcgcatac ggcacgcttc ctcaagccga tgctgtaatc gttaaggccg ctttctgagc
     3721 ggccttttcc tttcagagtt gtaccagcaa tttacgcttt tcttccggtc gtaaattcac
     3781 tgcctgctga taagacgcat ccaccagata atagatttgc gaatccggca gcgaaccgtc
     3841 gagatacacg gtgctccagt gcgctttatt cagatggcgg ctaggacgca catcgctgtg
     3901 ctgctgacgt agcagctccg ccagctccgg gctggttttc agcgaaacag ctgggcgatt
     3961 ttctacttct ttcaccatcg caaacagtac atcttccact ttgatctgcg tcgctttcca
     4021 gtcgttatgc acgctctgtt ctgcgcctag ttttgccatg caatattgta gcaactccga
     4081 aatggtcatt ttttactccc cttgtagtgt cgcgatgatg cgacgcgatc cgccgtggat
     4141 gcgatgttcc cccagccaga tgccttgcca ggtgccggtc tgaatacgcc ctttatgtaa
     4201 cggcaataca agcgatgttc cctgcattga ggatttgata tgagaaggca tatcgtctgc
     4261 tccctcatag tcatgctcat aatttccgtt gtcgggaacg gtgcggagga aaaaacgctc
     4321 catgtcgtgg cgtacggtgg gatcgcagtt ctcattaagt gtcagagagg cggaggtatg
     4381 ttgcagcaac agatgcagta agccgatgtt aacgcgcggc atattagcca gctgattcag
     4441 aatttcatcc gttaccagat gaaacccacg agatttggcg ctaagcgtga gcgtcttttg
     4501 ataccacatg tgctgctcct tgataaaact ctcttaatca gtttgcagca agacagcgaa
     4561 aggataaagg tgtgattaaa aaaacagcat tcgggagagc atcacgctct cccgctctgt
     4621 cagtattctg aattgacgat cacctcttca ccaaacgcac ccgcttgtgg caagggtttg
     4681 taggtagagt tggaggcgcg cagaatgcgg ataccacgag cgccgacatc gcgtgcggcg
     4741 gtaatatcat tatcagaatc gccataaaaa attcggatat ttttatcctg cagccattgc
     4801 gattttgtat tttgccctgg tttatcgccc gcaaagatca ccggattcat gttggtggca
     4861 ggaatatgaa aattatccgc cagcgttttt gaaaccgttt ctgttttcgt cggactacga
     4921 ccagtcacaa agaagatcgc gtcaccgcgg cgtacatgca tatcaatcag ctggcgagcg
     4981 acctcttttg gaatgctgaa ttcatcccag ccattgttca ttttttccca gaacacagga
     5041 tttttcagat aatcttcgct ttctggcgag aaggtttttt tgccgcgcca gaagcccggg
     5101 ctggaaaaaa gtaccgtgtc atcgatatca aaccccaccg ccattggcgg acgccctgcg
     5161 aggctatttt caatttgtgc gaccgaaacc caatgaattg gtgcctgttc agcaagcctg
     5221 gcaacgttag taccagggtt aagcggtgaa ggagatgagg ccagggcaac agcggaactg
     5281 tttagagcga acaataagca aacggcactg attgcctgtg tgatcttgcg catatttttc
     5341 cctaaatagt cagtatgttg aaacttttta ttgtgagatt tgttgcaaaa accatctgac
     5401 cataacgcca gctgcagggg aaaggaagga ttatctgcgg tttttgtgat gaaaagcagg
     5461 atgattatat ttaacccagt aaatacgttc ctgtcttttt ttgtataaaa aatgtttatt
     5521 actcgatgta aggatacatt aattttacgt ttattatgat gaatcctctg caaatgagcg
     5581 aaatttgata gaacatttat ctgctgtacg atttaatgaa cttaatgata cagcattaca
     5641 aaaacaatcg aagtttataa agatgattcc tgattgacca gaaccctatc ctacaatggt
     5701 gattaatttt attactcgcg atggagatga tgatatgaat atatcatata tgaatagcaa
     5761 caaaacgaca tctttacctg ttgagctaga tgcactaaat aataaagata tctcttatgc
     5821 caaagatttt tcttatgtca aagatttctt tttatacata gaaacacagt taaaaattgc
     5881 aaaagatttt tgtagacttg gagaagaagt atcaagttct attgcaagta aagtttttca
     5941 cgcatttatt gatttagtta acaaaatcag gggtaagaaa gactttatgt atatttgcac
     6001 gctttgctgt tttgccgagg aggttaaagg tgattattct cattacagga cctttttatt
     6061 tgatattggt aatcaatata aggttaaact tacacaaagc ggaaaaaaag agttctcttt
     6121 aacattagaa tttaacgata ctataattga atctcagaaa gtcacaggca ataaggcaaa
     6181 gcatattctt gaggatatag aaatattcta tcgtaacaaa cccgatactt attattaaat
     6241 ttaataatgg ttagatgtgc agaaattatt actccacacg aaaaaatagg gaaa
//
LOCUS       DAJTNN010000204         6284 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTNN010000204 DAJTNN010000000
VERSION     DAJTNN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.206"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..135
                     /locus_tag="OO150_003693"
     CDS             <1..135
                     /locus_tag="OO150_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5517685.1"
                     /translation="LTRLAGSDSGDEHAAVLFSLIGTCRLNNVEPEKWLRYVIEHIQD
                     "
     gene            complement(397..963)
                     /gene="ipgB2"
                     /locus_tag="OO150_003694"
     CDS             complement(397..963)
                     /gene="ipgB2"
                     /locus_tag="OO150_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5517686.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1245..1559)
                     /locus_tag="OO150_003695"
     CDS             complement(1245..1559)
                     /locus_tag="OO150_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517687.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSAQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(1637..1747)
                     /locus_tag="OO150_003696"
                     /pseudo
     CDS             complement(1637..1747)
                     /locus_tag="OO150_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2071..2748)
                     /gene="ospD1"
                     /locus_tag="OO150_003697"
     CDS             complement(2071..2748)
                     /gene="ospD1"
                     /locus_tag="OO150_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5517688.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGVPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(3119..3256)
                     /locus_tag="OO150_003698"
                     /pseudo
     CDS             complement(3119..3256)
                     /locus_tag="OO150_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3338..3636)
                     /locus_tag="OO150_003699"
                     /pseudo
     CDS             complement(3338..3636)
                     /locus_tag="OO150_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4225..4384)
                     /locus_tag="OO150_003700"
                     /pseudo
     CDS             complement(4225..4384)
                     /locus_tag="OO150_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4388..5542)
                     /locus_tag="OO150_003701"
                     /pseudo
     CDS             complement(4388..5542)
                     /locus_tag="OO150_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5562..5909)
                     /gene="tnpB"
                     /locus_tag="OO150_003702"
     CDS             complement(5562..5909)
                     /gene="tnpB"
                     /locus_tag="OO150_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5517689.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(5909..>6284)
                     /locus_tag="OO150_003703"
     CDS             complement(5909..>6284)
                     /locus_tag="OO150_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5517690.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
BASE COUNT         1615 a         1443 c         1344 g         1882 t
ORIGIN      
        1 ctgactcgac tcgcaggttc cgacagcggt gacgaacatg cagcggtgtt gttctcgctg
       61 atcggcacat gccgtctgaa caatgtggag ccagaaaaat ggctgcgtta cgttattgaa
      121 catatccagg actgaccggc aaaccgggta cgcgatctgt tgccttggaa agttgatctg
      181 acttctcagt aaatatcaat atgattctga cgagccgctt acctttgagc agagaaaggg
      241 gagagggatc ccacatccat gagctaggaa aatacaaaaa ctggctatac aacaattcaa
      301 tatattagga cttacttgcg aggagaaata tagcaccgct caggcaattc ctgggtattt
      361 acttttctta gtttctttac tcgggatgtt ctgtcatcag aaaggcgatt ctaaatttgt
      421 aatataatca ctcatattcc cacgtaattt ctttagatca atatattgca catttttctc
      481 aaaaaactga tcggaaataa gatttgcaac attgcttgtt gtatcatttt taacgcttct
      541 attcatccct tcacaaagtt cgtccatttt ctttttgaaa taactgcttc ctgatacaag
      601 atgatttatg gaactttgtg cagcattgat gtctagagaa atgtgataat ctttctcaac
      661 ctgtttaaat atctgtttac ggagattatc gccgattttt cggctgtgtt ctgcacaaaa
      721 cttatcaata cattggttta ccacccggga tacgtagact tttttttcat catttttcca
      781 atctgtaata ttttcaccga tttgttctga aacagagctc aaaatactag tatttatttt
      841 ggttgatgat atttttacta tccttttccc cattgtacag cggataatct catcaacctt
      901 tcctgaaaaa tatcttttgt gagataaact gattccaaaa ttattaaaag atgttccaag
      961 cattataatc actttgtcat gtaaaatagt aacattgtca ttattttagc tgattcaaac
     1021 aattctgtaa atgctttgag ttctttactc ccttggctca atgatggacc gtctggaatc
     1081 ccagagacta aactctctga gaaagagagt atgaaaaaac ttggggcagt ccctaaatga
     1141 agggaccaac agcggtaaaa taaccggcat cattcataat gatgccctgt tagttgtagt
     1201 tatgaagatt tacgtcatta aaattgctgt tcgtagtgtc agtcctatgg tctggcatca
     1261 gctgaggatc gtagccgata catcactggc ttcacgacat tcgtgttgag gctattcatg
     1321 aaaactccac gctgaaaacg cctttttgta taaacggtca cggtatgccc ggagccactg
     1381 ttgctgatga attcgataaa accttggcgt ttcttgaagc tatcgttaat gcggataatg
     1441 aaacaacgat tggtgagatt cgctcttttg ccgacaccct gggcgctgtc cggttcaatc
     1501 gcaacaaaat caaccggcag ctgagcaaac ccaaccttgc ctctctggcg ctggagcatg
     1561 aagtgattta gctgggacgt agccactgac agcttctata aaagtatgat cctacccacg
     1621 taatatgaac acaggcttaa gtgaggttct ggttttcaaa ttgttcagga ctgagacctc
     1681 cacaccaact gtgccgtcgt cagcgattgt aatcgcactt gatataatta aacactgtat
     1741 atttcatttg tggagtctcc agtctgaggg ggctttatgc attccgtatt gggcactatg
     1801 atgccagaaa tctgcaaggc tccacacctt cctgtatttc ccttcctcta tcacagtgca
     1861 ctttacttaa tagggtggga tgtgttcatt acatttattg tgttttttat gattgttttt
     1921 gcgtccagtg tcgttcgtca tgggaaactg gttctatggg tagtatcgct cagtctagtt
     1981 aactgtttgt ttctttttag aaattaagca gatcacacaa ttcggggaga gctccttcta
     2041 agtgcaattc tttgcagcta tttagcacac ttaaaacaac tcttgtattt tgtcagaaat
     2101 aatattaaac atgtcttgtt cattattgtt aagtctgtct gctatttctg taagttttat
     2161 cccactatga tggcatatat ttatatatgc catcatagcc tcgatattcc ctttccctgc
     2221 ggccagaaat aatcctggta ccccctcatt atctcttaca tccaatagtt taatcgtttc
     2281 ttttggtgat aaatcaaaag ttttaataaa atctccataa gcttgaatcg cagcggaatg
     2341 accttcttga agagctatga acaaagcaga ttccccagca ttatttcgtg ctgcaagaac
     2401 ttctagtttt tcctctttgg gtatttcggc tgccaacagc gattcaccgt atttacgcgc
     2461 tgcatcagca tttccatgcc aaatcaatga aaacaaatta tggcggttat actccttatt
     2521 attctttgtc ggaatggata tgctctgtat tttggccaat tttctgaaag aaacaccact
     2581 atatcccccc cctgattttt cttcattgat ggcgtgggat atagcggtat ttgtcacgtt
     2641 aatgatatta cgtttcattc ctttattttc attagcagta ttgctgccta ttattaggtg
     2701 catatttttg ttgtttgctg gatgtaatcc atagttattt attgacattt ttgttcatcc
     2761 tttttgctaa aaaaaacaat aacctatttt tgtaatgtaa tagatattag tctattgcta
     2821 atctaaaaaa accattgcca cattgaatgg ctatattcac caataaattc tttcgatttc
     2881 gtatattgac atatcgtaga tatatcttat ccctaagatt caataatcga gtccgtttac
     2941 cattctgtgc aagcgccttc actcagaagt gaccgtcaag gcgatcacca aacttgataa
     3001 taaagcagct cattgccata cgccagtcct taaggggcgt tgtccatttc tgtgatgctg
     3061 cctagattta actgcctgct ccggcttatc tacatctaaa tatgtgagcc taataccgct
     3121 acactttggc cagactatgt ttttctccgg gaagtgggct aaccgtctcc ataaagtgac
     3181 gaactttttt caacagtggc catattgaac ggcactgatg attacgggtt attgtctcat
     3241 gaagagccgg ccacatcact gacagcagtg acttcaggcc ttcagcacca gacagcctga
     3301 accagttaat ccatcgcccg acagatttag taatctaggg ctggtttttc ttcagctgtt
     3361 gcggttcgaa agtgccatta cgatcacgca gagtacggat ttccagaggt tcatctccag
     3421 tagtgataat cttcgttaag caaccattgc gggaattgct gccagatcta agctgatttt
     3481 tctcgcaccc aagatgatga gtcatattag cattgagtac ggtttcattt tcagcatcca
     3541 atcaaactga ctgagatctt caggattttt gagacttttg gctagttcat tagctaaagc
     3601 ccgcaactta ttagatgtca actgtttttc attcataaat tcaccttcat ttgatatcaa
     3661 attaaatagt tcaacatcag agaattatac aaacttatgt acaggctcct atgatcttcg
     3721 aatcctgaag attatagtga aaataagttt tcacgttgaa atcccacata gttagtatat
     3781 gataaataat catggatgac ctgattaacc tcttggggta gtgcttttat agaagaataa
     3841 atattctact aatttaatac atcacctaaa atagtattgt tttttatggt tggttgaagg
     3901 tcgtggtgat gatgttagtc cttttgagaa tattccagtg ccagatctta cttataataa
     3961 aggggcttgg cagtttggat aaactattgt tcttattcca ctaatacaaa ctttttgtaa
     4021 agtatattaa atatacttca tttcatactg aaatccccgg aagtattcaa tcaaacaatt
     4081 acaccataaa aggaagaggg ggccctaatt aagttgtcaa gcatgtttat gatacctgca
     4141 gggtataaat tttctggctg caagcagaca catataaaac gtctttattg atcctaccca
     4201 cataatatgg acacagtcgt aagtgaggct ctggttttca aattgttccg gactgagacc
     4261 accacaccaa cggtgccgtc gccagcgatt gtaatcgcac tcgatataac taaacactgt
     4321 tgcccgcact atttcccgat ggataatagt gttctccgtg gagacattcc attttcagcg
     4381 agtgaatttt catctgttgc tgtatccagt catacagtga ctgcatcagt ggcgcggctc
     4441 tggcttttct tgccgccaga cgctgttctg ctgaacatcc ccggacttct gcctcgatgg
     4501 catacagttc accgatacgc agcagggctt ccgttattct gccggattcg tacaacgccc
     4561 ggtaaccacc gtaagcatcg gcctgaagca ctccgctgta accggccaga tgattctgtg
     4621 gatggatacc tttccggtcc ggtgagtacg cgaaccagac cgccggaggc atttccgaac
     4681 cggcgttacg gtcatcacgg acatagaccc acagccgggc agttcttgtt ttaccactgc
     4741 ccggctcctg gaccggaacg gggatatcat ccgcatgaac tttaccgggc atcagcacat
     4801 actggcgcag gacgtcatac agcggctcca gcagttcagc aacggcaccg gtccagcgac
     4861 ccagtgtggc acggctcagc tccacgccct gacgacggta tatttctgac tggcggtata
     4921 acggcagata gcctgcatat tttccagcga caacatgggc cagaagcccc gctccggcat
     4981 aactgcgtgc aatgggtttt gaaggcaccg gtgcctgcac gatatggtca caccggcaac
     5041 aggccagttt cggacgttgt gtttcgataa ccttaaaggc gctgctgata agctccagct
     5101 gctctgacac atcacatccc agagaactga gttcgccacc acaggccggg cagcattcct
     5161 cttccggacg gatgacccgg gtttcacggg gaagtgaggc aggtaacggt ttacgggctg
     5221 aagactggcg caggggagat ggcagtaccg ggtcatattg ctcacccagc gtttccgcca
     5281 tttcttcctg aagtgcgctg attcgctcct gtgcttccag tatctgccgt tcggtttttg
     5341 cgcgaagttt ttctgagctt taaccgaact gcatgcgctg cagtttcgca accagcgcct
     5401 tcagccggtt gatttcggaa gcataagccg ccacccgctg tgagagcagg cggttgtatt
     5461 tcgccatctg gcggatggtg tcctgttgcg tctgcaacag tgcccgcagg cgggcattct
     5521 catgagcaag tgaggtgtcc atatcctcac tttacaacgg gttatatgcg gatgccagcg
     5581 cgttccgttc gtttcgggtg tttccagtcg atgccctcaa gaagcatgga taactgggcc
     5641 ggagtaaggt gcaccttgcc gtcacgggtg accggccaga cgaagcggcc ccgctccagg
     5701 cgtctggtaa agaggcacag tccgtcactg tcagcccaca acacttttat ctggtcaccc
     5761 cggcgtccgc ggaagatgaa cagatgcccg gagaacgggt catccttcag gacgttctga
     5821 acttttgagg ccaggccgtt aaagccattt cgcatatcgg tgatacctgc aaccagccag
     5881 atacgcgaac ctgcagggaa agatatcatc agtggctgct cccttttatt tcgcggataa
     5941 gtatctgtaa taacgccggc gtcagtttac ctttaagcct gagagttccg gccggcagaa
     6001 ccagctcaca gcacagactg tcggacggtg tatttatctg ctctggttcc tgtgccggag
     6061 ccgggatttt attatccggc tccggcgtta acgtcacggg aagcagtgca ggcatatttt
     6121 ttccggaagg cagcaggcca cctttccggt attgatggcg ccagttgaag agcaggttat
     6181 cgttgattcc gttttcacgg gcgatctgcg ccacacaggc tccgggctgc agtgactgct
     6241 ccactaaggc gattttaaat tcataaggaa aattaggccg ccgg
//
LOCUS       DAJTNN010000205         6242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTNN010000205 DAJTNN010000000
VERSION     DAJTNN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.207"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(182..967)
                     /gene="fliR"
                     /locus_tag="OO150_003704"
     CDS             complement(182..967)
                     /gene="fliR"
                     /locus_tag="OO150_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5517691.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(976..1245)
                     /gene="fliQ"
                     /locus_tag="OO150_003705"
     CDS             complement(976..1245)
                     /gene="fliQ"
                     /locus_tag="OO150_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5517692.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(1255..1991)
                     /gene="fliP"
                     /locus_tag="OO150_003706"
                     /pseudo
     CDS             complement(1255..1991)
                     /gene="fliP"
                     /locus_tag="OO150_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            complement(1991..2356)
                     /gene="fliO"
                     /locus_tag="OO150_003707"
     CDS             complement(1991..2356)
                     /gene="fliO"
                     /locus_tag="OO150_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5517693.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(2359..2772)
                     /gene="fliN"
                     /locus_tag="OO150_003708"
     CDS             complement(2359..2772)
                     /gene="fliN"
                     /locus_tag="OO150_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5517694.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMHRLSR"
     gene            complement(2769..3773)
                     /gene="fliM"
                     /locus_tag="OO150_003709"
     CDS             complement(2769..3773)
                     /gene="fliM"
                     /locus_tag="OO150_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5517695.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(3778..4242)
                     /gene="fliL"
                     /locus_tag="OO150_003710"
     CDS             complement(3778..4242)
                     /gene="fliL"
                     /locus_tag="OO150_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5517696.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALNTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(4347..5474)
                     /gene="fliK"
                     /locus_tag="OO150_003711"
     CDS             complement(4347..5474)
                     /gene="fliK"
                     /locus_tag="OO150_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5517697.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(5471..5914)
                     /gene="fliJ"
                     /locus_tag="OO150_003712"
     CDS             complement(5471..5914)
                     /gene="fliJ"
                     /locus_tag="OO150_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR5517698.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERLSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(5933..>6242)
                     /gene="fliI"
                     /locus_tag="OO150_003713"
     CDS             complement(5933..>6242)
                     /gene="fliI"
                     /locus_tag="OO150_003713"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5517699.1"
                     /translation="RLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFKQLLSSFQ
                     RNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLERIFPTVS
                     "
BASE COUNT         1468 a         1638 c         1656 g         1480 t
ORIGIN      
        1 tttcaggcgg acttactatc ccgtaaggtg ttgtataatt tgcctggaat tgtcttaaag
       61 taaatgttgc gatatgtgag tgagcttaaa acaaatattt cgccgcagga gtatcctgga
      121 agatgttcgt aggtattatt tttcggataa tccttaggat aacatgataa acgttacgga
      181 attatattaa tggcaattca ctaataatat cagccagcaa attaaacatc tcactgaata
      241 aatgttcgca aaaaggtgca attaacggca ttaatgccgc cattaaagag ataccgacag
      301 tcagagttaa tggaaatcca ataacaaaaa tggataattg cggggccata cgattcagta
      361 aacctaatgc cagattcagg gtcagcagca gagtaatgag cggtaacgcc agcatcagcc
      421 cgttaaggaa aatcaaactc cccgctttgg tgagtgccag aaacgcattg ctgttcaacg
      481 gttcgccacc aattggcagg gtgtgaaagg tatcgaccag cagtgaaatc aaccataaat
      541 gaccgttaaa tgtcaggaac agcagtaacg ccagcatatc catgatacgc gctaaaacgg
      601 gcatattgag atggctggcc ggatcgacaa acgtcgcaaa tgacagcccc atttgcagac
      661 cgataatttc gccagcggtt cgcacagcgg caaaggcaaa ttgcatggta aaaccaagcg
      721 caatgccgat caggatctgc tgcacggcca gccacagagc aaagaacgaa aaaacaggaa
      781 catcgttggc aggtaatgat ggggcaatga cgaacgtgat catcattgcc agccccagtt
      841 ttacccgttt cggcacgctg cgttcgctca gaattggtgc ggtagaaatg agcgccagca
      901 cgcgcagtaa cggccagaag tacaggctta accaggaaag ccattgatcg cttgtcacct
      961 gcatcattgt acggcctacc cgatgatata cggcaggtta gtgaacaagg tgcggacgta
     1021 atccagcaac agattgagca tccacggtcc ggcaataata atggcaataa atacggcgat
     1081 gatttttgga ataaacgaca gcgtcatttc gttgatctgc gtggcggcct gcaaaatact
     1141 gatgataagg cccgtgacca gcgccaccag caacagcggg gcagccagtg ccagcgcgac
     1201 tttcatcgct tcagtcccca tcatcatgac cgattcaggt gtcattttgc ctctctagct
     1261 gtaaaagctc tgcgccagcg aaccgaccag caattgccag ccatccacca gtacaaacag
     1321 catcagttta aagggcagag caatggtggc ggggggacca tcatcatccc caatgccatc
     1381 aacacgctgg caatcaccag gtcgataatc aaaaaaggaa tgaaaatcgt gaagcctatc
     1441 tgaaatgcgg ttttcaactc gctggtcacg taggccgggc gcaaaatgcg catcggcacg
     1501 gcttcaggtc cctgcaacgg gccggtattc gctagtctgg caaacaaccc taaatctgcc
     1561 tcacgggtct gacgcagcat aaactcacgt agcggctgcg cccctttttc cagcgcctcc
     1621 tgcattgata ttttctcttc gctgaatggc tggtacgcat cgacataaat tttgtcgatc
     1681 accggtgaca taataaaaaa ggtcaaaaat agtgccagcc ccagcaatac ctggttaggt
     1741 ggcgcggagg gcgttcccag cgcgttacgc aataaaccaa aaacaatgat gatgcgggtg
     1801 aaactggtca tcatcagtaa aattgccgga ataaacgtca acgaggtgat gaacaccagc
     1861 gtctgcaccg ggagcgacca gctttgtcca ccgccaggca gcggctggct ggtgataccc
     1921 ggcagttgcg cgaaggcgag gggcgtaacc agccagagaa ggacaggtgc gacagacaac
     1981 aaacgacgca tcaggatctc ccactacgct taagcaaatt tttcatgacc gactgaaaat
     2041 cggtctgcgg tatctcttct gttggtgctg aagggggaag tttatgcagc agattgattt
     2101 gccctgcggt aacgccgagc accagccgtg catcttccac atcgaccacc acaacccgtt
     2161 cacgcgcgcc cagcgaagca ctggcgctaa ttttcagacc gttaacgcca gtgcgtttcg
     2221 gggcaaatcc caaccgtttt acctgccagg cagcagcgag gatcagggca ataatggcga
     2281 tgagtgcgcc gctgacctgc agcagtggcg cagcagaaac cggcgtggaa gattgcacag
     2341 tagcgtggtt attcatcact aacggctcag gcgatgcatt cgctcagacg gagtaatgat
     2401 atcggtgatc cgcacgccat atttatcggc aacgaccacc acttcgccct gggcgattaa
     2461 ataaccgttg atcagaatat ccagtggttc gcccgccaga ccgtccagcg ccacgacgga
     2521 cccttgcgtc agacgcaaca gctctttgat ggtcatccgc gtacggccca gctcgacggt
     2581 cagcttgacc ggaatatcca ttatcaggtc gatatcctgc aacgttccgc tgacatcacc
     2641 accgccaaat tgctggaaca ccgcgtcggc agcgcttttg ctgctggttg atttttgttc
     2701 gctcaacgct tcagcccaca gatcgtccat tgcgccgttg ttgtcatcgg ccggattatt
     2761 catgtcactc atttgggctg ttcctcgttc agagaattta aaatcgggtt aatcaaatgt
     2821 tctatccgta acgcatactg accgttgaga gtgccgtact gactggtcag caccgggacg
     2881 ccgtcaacat gggcgatgat gcgatcaggt ttttctatcg gcaggacgtc gccgggtttc
     2941 agttttaaaa tctgcgacag gcgtagcgag atatcggcaa agttggcgac cagctccagc
     3001 tgtgaatgct gcacctggcg caccaggtta tcgcgccagt tctgatcttc attacgcgag
     3061 ttttccagcg gcgggttaac caacaattcc cgtagcggct cgatcatgct gaatggcaga
     3121 cagatattaa attcgccggt caggttgcca atctccacat ggaacggcgt gttaaccaca
     3181 atgtcgttcg gcgaggtggt gatattggta aatttcacct gcatttccga acgcacgtac
     3241 tcaacttcca gcggattaat cgccttccag gcgtcgctat agccttcaag cgccagcttt
     3301 aacatgcggt tgatgacgcg ctgttcggta tgggtaaact cgcgaccttc cactttggtc
     3361 gggaagcgtc catcgccgcc aaacaggtta tccacggcga taaacaccag actcggtgaa
     3421 aacaccacca gccctgtgcc gcgcagcggt ttcagatgga taaggttcag gttggtcggc
     3481 accggcaggt tgcgggcaaa ttcatggtac ggctgaatgc ggatggcccc gacggttata
     3541 tccgggctac gacgcagcag gttgaacagc cccatacgaa aatggcgggc aaagcgctca
     3601 ttaatgattt ccagcgcctg caaacgttcg cgcacaaccc gtcgttgggt attcggatcg
     3661 tacggacgaa tgtcactttc gccgctaaca ctggctgtcg gttcgtcttt gacttcgctg
     3721 tcaccattca acagcgcatc aatttcagct tgagaaagaa tactatcgcc catgtcgtta
     3781 tcgcagaata aaagcggtat acagcacgtc ggtgacatcc tgtttcggtt gcccggcaac
     3841 aagcggggtg gaaagcgtgg ttttaatctc ggcaatcagg tttttcttgc cttcttctgt
     3901 cgccagtacg gcagcatcct gacgcgaaaa cagtaacagc aaacggctac ggacttccgg
     3961 caaatactca ctcagccgcg agcgggtagc ttcatctttc aggcgcaggg ttatgccgat
     4021 ataaagcacg cgatccgcat cgcccaaatt gaccgtgaag gtattcagcg cgtagaagac
     4081 cggggagggt acgacgcgtt gctgcgcttt gtcgtcagcg gcaacctgat gcgaatgcca
     4141 gtagctgtaa cctgcgctgg cacaggccgc gagggtaatg aataccagaa tcgggatcca
     4201 aagcgatcgc ttgcttttct tgcttatcgc gtaatcagtc atgtgttgcg gtcttcctgt
     4261 gtcgctactg cttatcggct aattatcctg cgttttgagc gcaacaaagc ggggaaaaga
     4321 cgcggattac ggtgctacct ctgacgttag gcgaaaatat caacgccgct gttgcctgtt
     4381 acacgccctt gtaaagagac gggaaccgga agcgtatcgt cgtcttcccc cgccagaggt
     4441 tcatggttta ctgtgcgttg gctttgctgt tgttgggaag cggcctgctg ctgaccacta
     4501 aagctttcgc cactgatgtt gctttgccct aactgaatgc cactttcggc cagctgcgta
     4561 cgcagtaccg gaagcgctgc ttccagcgcg gcgcgtacat gctgatgcgg tgaaaccatc
     4621 tggatttgcg cctggttatc atccactttg agggagattt gcacttcacc taaatcctgc
     4681 gggtgcagac gcaactctgc actttgttgc ccctggcggg tgaacagcga aatatgctgg
     4741 cttaatgatt gttgccattc gtgagaaccc agcggtgcac tcaaaacagg cgcggcgacg
     4801 gtgggcagtg gctgtgtctg gtgtggagta attagcgggc tggcggcagc ggtcaccggt
     4861 gacggtgtgc tgatgacttc cgctttactc tgggcttctg ctaccagcgg tgttaatggc
     4921 tgagctggcg tgccgggggc atcatcaggc tgtgctgttg tgagttgcgc agaagtcagt
     4981 tttgtgaaga gcgttggttt ctctgccggt aacacggttg acggcacatc agtcactttg
     5041 ggcgtattgt caaaacccgg caacatcgca aaaagggcgc tcaggcttgc ggtgacgtct
     5101 tcattcagat catccgcttt ttcgtctttt gtcgtatttt ggtcagccac cgcagccaac
     5161 gtcatcgtct gagcggtggt taacggtgtt gatgtggatt gttcgtcgtt gatgacaggc
     5221 agtgtttcat ccacggggat cagtaaatcc gcttgttgcg cgtcggcaag aatatcgctg
     5281 atcagcggct cgccttttgt cgtgggctta tctgttgcca ccagcaactg gggggccgct
     5341 ttgtcggtag ttgtctcgcc tgctaatgct tcgctcaaca acgcgagaaa atcttgtgca
     5401 gcatcgctgg ctttgccgcc aggcaatgtg gtggtgtcaa cgtcggcggt aattaagggc
     5461 gctaagcgaa tcattcaggt ttcctcatgg cggcgcgctg ggcgaactca tccatctttt
     5521 tctgatcgag gcggttttct gcaagcagtg ccgccgtgga tagccgttcc tgcagtgtct
     5581 gccaggcctg caaacgttgt ttcttttctc gccagctgtt cagggcaatg tcaactttct
     5641 gcgtccactg attaagttgc tggcgatgct gagtaatggc tttttccagc gtctggataa
     5701 actgctgata gttgatccag cggttgctgg tcatcccggc actcatatcg ctgttgaggt
     5761 tattgcgata ttcattctga taatcgatca gcattttgag ctgttcttcc gcctgctgac
     5821 acccgcgacg catttcaccc agcaggcgcg cggcatcctc tacctctttt tctgccagat
     5881 ctttcagggt cgccagcgcg ccatgttctg ccatctgccg ttatctcctg ggttatgaca
     5941 ctgtcgggaa aatacgctcc agcccctgga gagacgcttc ccagtccgcg cgttcaaaaa
     6001 tgccttgttg caaatagccc tccagctgcg gccacagggc gatggctttg tcgagcatcg
     6061 gatcgctgcc tttggcatac gcgccgacgc taaccagatc gcggttacgc tgaaaactcg
     6121 acaacagctg tttgaaggtg cgcactcgcg cgtaatgttg ctcactgatc aacgccgtca
     6181 ttgcgcggct gatcgacgct tcaatatcga tagccggata gtgcccggct tccgccagtc
     6241 gg
//
LOCUS       DAJTNN010000206         6086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTNN010000206 DAJTNN010000000
VERSION     DAJTNN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.208"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..226)
                     /gene="yneK"
                     /locus_tag="OO150_003714"
     CDS             complement(<1..226)
                     /gene="yneK"
                     /locus_tag="OO150_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5517700.1"
                     /translation="MVTPISISNYIFLPDDFPVRNIAPQVKEVLKNFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFR"
     gene            complement(304..1185)
                     /locus_tag="OO150_003715"
     CDS             complement(304..1185)
                     /locus_tag="OO150_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5517701.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTIRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            1286..2674
                     /gene="sad"
                     /locus_tag="OO150_003716"
     CDS             1286..2674
                     /gene="sad"
                     /locus_tag="OO150_003716"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR5517702.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2738..3664
                     /gene="glsB"
                     /locus_tag="OO150_003717"
     CDS             2738..3664
                     /gene="glsB"
                     /locus_tag="OO150_003717"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5517703.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3664..4023
                     /locus_tag="OO150_003718"
     CDS             3664..4023
                     /locus_tag="OO150_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5517704.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            4162..5580
                     /locus_tag="OO150_003719"
     CDS             4162..5580
                     /locus_tag="OO150_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5517705.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            5807..6046
                     /locus_tag="OO150_003720"
                     /pseudo
     CDS             5807..6046
                     /locus_tag="OO150_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
BASE COUNT         1452 a         1400 c         1630 g         1604 t
ORIGIN      
        1 cccgaaaaga agtcctctga atatatgata agttatcaag gttgttaaat atcttttttg
       61 tggctgagtt ggtgtttaaa gacgtgcgcc actcttcatc acatattatg gtactgagtg
      121 catcaataaa attctttaaa acttctttta cctgaggtgc aatattacgt acgggaaaat
      181 catcaggaag aaatatgtaa ttactgatgc ttattggcgt aaccatattt gccccatata
      241 gtcataagtc atcggaacat cactatatag ggcatttgag ctgcaagaac tatcaaaaat
      301 agctcattgg taaccctgtt ttgccgagtc aggcacatcc agtagttgaa taaatgcctc
      361 aagcggacgc gttttcgcac cacgacgcca gaccagccag gttgttaacc aacgccattg
      421 ctcagctaac ggccacgctt caacctggtg atgccccggc atactttcca gcatagagcg
      481 tggaataagc gcaatgcctg ctcctgcgac cacacaggcc aacatcccgt gatacgactc
      541 catctcgtga atagttcccg gagcggcacc gtcagcatga aaccagctct cgaagtggcg
      601 acgatacgaa caattggcgc ggaaggcata aatgttactg ccgttaacct gactggcgcg
      661 gattactggc gcatgacctt gtggcgtgac gatcatcagc tcctcgcggt ataccggtat
      721 cccgtcaatg gcagtatggt taatgggccc atcaataaat gccgcattca gttttccctc
      781 cagcactcct tccagcatag tgccggaagg gccggtggaa agtgaaaact gaattttggg
      841 ataacgatgg ttgaattccg ccagcgtggc cggaatgcgc actgctgcgg tgctttccag
      901 cgaaccaaga gaaaataaac cttgcggctc atcgccagcg acaacgctcc gcgcttcatc
      961 cactaacgcg agaatttgtt ggctatagcg taaaaagtta tgcccggcag gagagagacg
     1021 taaacgctga ttctcgcgaa taaacagatc tacccccagt tctgtttcca gctggcgtag
     1081 acggatagtg agattcgacg gcacgcgaag cactattgct gctgcctggg ttatgctgcc
     1141 agcttcggca accgcgttga acatctccag ttgggtcaga tccataatat tctcgattcg
     1201 tgaataagtg gcttaatatt attcatttta aagcaagagt aaatctgcgt atcttcatac
     1261 catgactcat aaaggagata ccccgatgac cattactccg gcaactcatg caatttcgat
     1321 aaatcctgcc acgggtgaac aactttctgt gctgccgtgg gctggcgcta acgatatcga
     1381 aaacgcactt cagctggcgg cagcaggctt tcgcgactgg cgcgagacaa atatagatta
     1441 tcgtgctgaa aaactgcgtg gtatcggtaa ggctctgcgc gcccgtagcg aagaaatggc
     1501 gcaaatgatc acccgtgaaa tgggcaaacc aatcaatcag gcgcgcgctg aagtggcgaa
     1561 atcggcgaat ttgtgtgact ggtatgcaga acatggtccg gcaatgctga aggcggaacc
     1621 tacgctggtg gaaaatcagc aggcagttat tgagtatcga ccgttgggga cgattctggc
     1681 gattatgccg tggaactttc cgttatggca ggtgatgcgt ggcgcggttc ccatcattct
     1741 tgcaggtaac ggctacttac ttaaacatgc gccgaatgtg atgggctgtg ctcagctcat
     1801 tgcccaggtg tttaaagatg cgggaatccc gcaaggcgta tatggctggc tgaatgccga
     1861 caacgacggt gtcagtcaaa tgattaaaga ttcgcgcatt gctgctgtca cggtgaccgg
     1921 aagtgttcgt gcgggagcgg ctattggcgc acaggctgga gcggcactga aaaaatgcgt
     1981 actggaactg ggcggttcgg atccatttat tgtgcttaac gatgccgatc tggaactggc
     2041 ggttaaagcg gcggtagccg gacgttatca gaataccgga caggtttgtg cagcggcaaa
     2101 acgctttatt atcgaagagg gaattgcttc tgcatttacc gaacgttttg tggcagctgc
     2161 ggcagccttg aaaatgggcg atccccgtga tgaagagaac gctctcggac caatggctcg
     2221 ttttgattta cgtgatgagc tgcatcatca ggtggagaaa accctggcgc agggtgcgcg
     2281 tttgttactg ggcggggaaa agatggctgg ggcaggtaac tactatccgc caacggttct
     2341 ggcgaatgtt accccagaaa tgaccgcgtt tcgggaagaa atgtttggcc ctgttgcggc
     2401 aatcaccgtt gcgaaagatg cagaacatgc gctggaactg gctaatgata gtgagttcgg
     2461 cctttcagcg accattttta ccaccgacga aacacaggcc agacagatgg cggcacgtct
     2521 ggaatgcggt ggggtgttta tcaatggtta ttgtgccagc gacgcgcgag tggcctttgg
     2581 tggcgtgaaa aagagtggct ttggtcgtga gctttcccat ttcggcttac acgaattctg
     2641 taatatccag acggtgtgga aagaccggat ctgacgcgac atattcagct ctgatatact
     2701 cgcaggtctt ttcagacctg cggtccagga gtagaaagtg gcagtcgcca tggataatgc
     2761 aattttagaa aacatcttgc ggcaagtgcg gccgctcatt ggtcagggta aagtcgcgga
     2821 ttatattccg gcgctggcta cagtagacgg ttcccgattg ggcattgcta tctgtaccgt
     2881 tgacggacag ctttttcagg ccggagacgc gcaagaacgt ttttccattc agtctatttc
     2941 caaagtgctg agtctcgttg tcgccatgcg tcattactcc gaagaggaaa tctggcaacg
     3001 cgtcggcaaa gatccgtctg gatcaccgtt caattcttta gtgcaactgg aaatggagca
     3061 gggtattccg cgtaatccgt ttattaatgc tggtgcgctg gtggtctgcg atatgttgca
     3121 agggcgatta agcgcaccac ggcaacgtat gctggaagtc gtgcgcggct taagcggtgt
     3181 gtctgatatt tcatacgata cggtggtagc gcgttccgaa tttgaacatt ccgcgcgaaa
     3241 tgcggctatc gcctggctga tgaagtcgtt tggcaatttc catcatgacg tgacaaccgt
     3301 tctgcaaaac tactttcatt actgcgctct gaaaatgagc tgtgtagagc tggcccggac
     3361 gtttgtcttt ctggctaatc aggggaaagc tattcatatt gatgaacctg tggtgacgcc
     3421 aatgcaggcg cggcaaatta acgcgctgat ggcgaccagt ggtatgtacc agaacgcggg
     3481 ggagtttgcc tggcgggtgg ggctaccggc gaaatctggc gttggtggcg gtattgtggc
     3541 gattgttccg catgaaatgg ccatcgctgt ctggagtccg gaactggatg atgcaggtaa
     3601 ctcgcttgcg ggtatcgccg tccttgaaca attgacgaaa cagttagggc gttcggttta
     3661 ttaatgcagt ctctcgatcc actcttcgcg cgtttatccc gttcaaaatt tcgctctcgc
     3721 tttcgtctgg gcatgaaaga gcgtcagtat tgcctggaga aaggcgcgcc agtcatcgaa
     3781 caacatgcgg cggattttgt cgctaaacgt cttgctccgg cattaccggc taatgacggt
     3841 aagcaaacac ccatgcgcgg gcatccggtg tttatcgctc agcacgccac cgcgacctgc
     3901 tgtcgcggtt gtctggctaa gtggcataac attccgcagg gtgtttcgtt aagcgaagaa
     3961 caacaacgtt atatcgtggc ggtaatctat cactggctgg ttgtacagat gaatcagcca
     4021 tgagaattgc ggaaaacgat aaaatagcat tcccagggta cctttatttg ccgccgctgt
     4081 tagcgaacat gctatccttt atgagaattt acttattacg ttgagataat tgccacccgc
     4141 aattgtatgg acagataatt aatgcatgta caccccattt caactttccg tttgttccag
     4201 gaaggtcatc tgctacgtaa tagcatcgct atttttgcgc taaccacgct gttctatttt
     4261 attgttgcag agttacggct ggttcacgaa ctttctcttt tctggccgct gaatggcgta
     4321 atggcggggg tgtttgcccg ctatgtctgg cttaatcgac tgcattacta tgcgatcagt
     4381 tatgtggcga tgctggttta tgatgccata accaccgaat ggggactggt ttcactggtt
     4441 atcaatttct ccaatatgat gtttattgtt accgtcgcct tactggtcgt gcgggataag
     4501 cgtctgggga aaaataagta tgagccagta agtgccttac ggctatttaa ttactgtctg
     4561 attgccgcat tattatgcgc tattgtcggg gcgattggtt cggtcagtat tgatagtctg
     4621 gatttctggc ctttgcttgc ggactggttc agtgagcaat tctcaacggg cgtgttgatc
     4681 gtgccttgta tgctgacgtt ggctattcct ggagtgctgc cgcgctttaa agcagaacag
     4741 atgatgcccg ccatcgcgct tattgtgtcg gttattgcct cggtagtcat tggcggagcg
     4801 gggagtctgg cgttcccgct gcctgcatta atctggtgtg cagtgcgcta tacgccgcag
     4861 gtaacatgtc tgttgacctt tgtcaccggt gcggtggaaa tcgtactggt ggcaaattcg
     4921 gtgattgata tctcggtcgg ttcgccgttc tccattccgg aaatgttctc cgcacgtctc
     4981 ggtattgcca cgatggcgat atgcccaatt atggtttctt ttagcgtggc agcgatcaat
     5041 tcgctaatga agcaagttgc gctgcgagcc gactttgatt ttctgactca ggtttactca
     5101 cggtccggtc tttatgaggc gctgaaaagt ccatcgctga aacagacaca acatctgacg
     5161 gtcatgctgc ttgatatcga ctatttcaaa agcattaacg ataactatgg acatgaatgt
     5221 ggcgataaag tgttaagcgt gtttgcccgg catattcaga agattgtcgg tgataagggg
     5281 ctggtggcgc gcatgggcgg cgaagaattt gctgttgcag tgccgtcggt gaatcctgtc
     5341 gatggtctgc taatggcgga aaaaatccgt aaaggcgttg aactgcaacc attcacctgg
     5401 caacaaaaaa cgctttatct cacggtaagt attggcgtcg gtagtggtcg cgcatcgtac
     5461 cgaacgctga ccgatgattt taataaattg atggtcgaag ccgatacgtg tctgtatcgc
     5521 tcgaagaaag atgggcgcaa ccgtaccagc actatgcgtt acggtgagga agttgtctga
     5581 catctttatt cattccgcgc gatttgaatg caatgtgttc taccacttta tgatccagga
     5641 aaaggcgtat ttcctcgatt aaaccatgat ccgcgccaca cttttctcat aaaactgctc
     5701 attaatcatc aggagttgat caaaacaggg gttcggcgta ttaaattttg ctaacctgtc
     5761 gtaccaattc agacgggttg tatgactaat cagaagggaa cccattgtga aaacactaaa
     5821 tcgtcgcgat attcccggtg cacagtatcc agaacgtatc attcagtttg gtgaaggtaa
     5881 cttcctgcgc gcctttgttg actggcaaat cgatctcctg aatgagcaca ccgatctgaa
     5941 ttctggcgtg gtcgttgttc gtccgattga aacttcattc ccgccgtcac tgagcacgca
     6001 ggatggtctg tacaccacca ttatccgtgg cctgaatgag aaggggtgaa gtggtcaaca
     6061 aaaactggcc accgagttag agtttt
//
LOCUS       DAJTNN010000207         6060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTNN010000207 DAJTNN010000000
VERSION     DAJTNN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.209"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1357
                     /locus_tag="OO150_003721"
     CDS             <1..1357
                     /locus_tag="OO150_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5517706.1"
                     /translation="QLALGRYTLTVRAVNAWGQQGDPASVSFRIAAPVAPSRIELTPG
                     YFQITATPHLAVYDPTVQFEFWFSETRITDIRQVETTARYLGAGLYWIAASINIKPGH
                     DYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDN
                     ASRLDEFSKEWKDANDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAA
                     NRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTA
                     KNADISGNVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGT
                     ITVRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQS
                     TTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISI
                     S"
     gene            1424..2023
                     /locus_tag="OO150_003722"
     CDS             1424..2023
                     /locus_tag="OO150_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5517707.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            2088..3492
                     /locus_tag="OO150_003723"
                     /pseudo
     CDS             2088..3492
                     /locus_tag="OO150_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            3441..3593
                     /locus_tag="OO150_003724"
                     /pseudo
     CDS             3441..3593
                     /locus_tag="OO150_003724"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            3570..3749
                     /locus_tag="OO150_003725"
                     /pseudo
     CDS             3570..3749
                     /locus_tag="OO150_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            4464..5111
                     /locus_tag="OO150_003726"
                     /pseudo
     CDS             4464..5111
                     /locus_tag="OO150_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            5273..5695
                     /locus_tag="OO150_003727"
     CDS             5273..5695
                     /locus_tag="OO150_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5517708.1"
                     /translation="MAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVT
                     WLSPEGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIA
                     TEEETSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(5750..>6060)
                     /locus_tag="OO150_003728"
     CDS             complement(5750..>6060)
                     /locus_tag="OO150_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCR5517709.1"
                     /translation="SISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDG
                     VVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK
                     "
BASE COUNT         1601 a         1500 c         1686 g         1273 t
ORIGIN      
        1 gcagctggcg ctgggacggt acacgctgac agtccgggcg gtaaatgcgt ggggacagca
       61 gggcgatccg gcgtcggtat cgttcaggat tgccgcaccg gtagcaccgt cgcggattga
      121 gctgacgccg ggctattttc agataactgc cacgccgcat cttgcggttt atgatccgac
      181 ggtacagttt gagttctggt tctcggaaac gcggattacc gatatcaggc aggttgaaac
      241 cacagcccgc taccttggcg cggggctgta ctggatagcc gccagtatca atatcaaacc
      301 gggccatgat tattactttt atatccgcag tgtgaacacc gttggcaaat cggcattcgt
      361 ggaggccgtc ggtcgggcga gcgatgatgc ggaaggttac ctggattttt tcaaaggcaa
      421 gataaccgaa tcccatctcg gtaaagagct gctggaaaaa gttgatctga cggaggataa
      481 cgccagcaga ctggatgagt tttcgaaaga gtggaaggac gctaacgata aatggaatgc
      541 gatgtggggc gtcaaaattg agcagaccaa agacggcaaa cattatgtcg cgggtattgg
      601 cctcagcatg gaggacacgg aggaaggcaa gctgagccag tttctggttg ccgctaaccg
      661 tatcgcgttt attgacccgg caaacgggaa tgaaacgccg atgtttgtgg cgcagggcaa
      721 ccagatattc atgaatgacg tgttcctgaa acgcctgacg gctcccacca ttaccagcgg
      781 tggaaatccg ccggcatttt ccctgacacc agacggaaag ctgaccgcta aaaatgcgga
      841 tatcagcggt aatgtgaatg caaattcagg gacgctcaac aatgtcacga ttaatgaaaa
      901 ctgtcagatt aaggggaaac tgtcagccaa tcagattgaa ggcgatattg tcaaaacggt
      961 cagcaagtct ttcccccgca cgaacagtta tgccagtggc accatcacgg taagaatcag
     1021 tgatgatcag aaatttgacc ggcaggtcat gataccgcca gtgttattcc gcggtggtaa
     1081 gcatgagaat ttcaacagta ataaccaaca gtcatactgg tattcaacct gccggttaag
     1141 agtgacccgc aatggtcagg agatttttaa tcagtccacg acggatgctc agggcgtatt
     1201 ttcctcagtt atagatatgc ctgccggaca ggggacgctg acactgacat tcaccgtatc
     1261 ttcatcagga gcgaataact ggacaccaac aaccagtatc agcgatctgc tggttgtggt
     1321 gatgaaaaaa tccacagcag gtatcagtat cagctgaatt ttataaccca gaacgggcgt
     1381 cagaaatgac gcctttttta ttgcagaaaa gcgagaggta attatgcgta aactttatgc
     1441 cgccattttg tccgcagcca tttgtctggc cgtatccggt gcgcctgcat gggcgtctga
     1501 acatcagtcc acgctgagcg cggggtatct tcatgcccgg acgaacgttc ccggcagtga
     1561 tgatctgaac gggattaacg tgaaataccg ttatgagttt acggacacac tggggatggt
     1621 gacgtcgttc agctatgcag gagacaagaa tcgccagatt acccgttaca gcgatacccg
     1681 ctggcatgaa gattccgttc gtaaccgctg gttcagcgta atggcggggc cgtctgtgcg
     1741 cgtgaatgaa tggttcagcg cgtatgcgat ggcgggtgtg gcttacagcc gtgtgtcgac
     1801 tttctccggg gattatctcc gcgtaactga caacaagggg aaaaagcacg atgtgctgac
     1861 cggaagtgat gacggtcgcc acagcaacac gtctctggcg tggggagctg gcgtgcagtt
     1921 taacccgacc gaatccgtgg ccattgatgt cgcttatgaa ggctccggca gtggtgactg
     1981 gcgcactgac gggttcatcg tgggtgttgg ttataaattc tgattagcca ggtaacacag
     2041 tgttatgaca gcccgccggt tcaggcgggc ttttttgtgg ggtgaatatg gcagtaaaaa
     2101 tttcaggtgt actgaaagac ggcacaggaa aaccggtaca gaactgcaca atccagctga
     2161 aagcaaaacg taacagtacc acggtggtgg tgaacacgct ggcatctgaa aatccggatg
     2221 aagccgggcg ttacagcatg gacgttgagt acggtcagta cagcgttatt ctgttggtgg
     2281 aaggattccc gccgtcacat gccgggacca tcaccgtgta tgaagattcc cgacccggta
     2341 cgctgaatga ttttctcggt gccatgacgg aggatgatgc ccgtccggag gcactgcgcc
     2401 gttttgagct gatggtggaa gaggtggcgc gtaacgcgtc cgtggtggca cagaacacgg
     2461 cagccgcgaa gaagtcagcc agtgatgcca gcacatcagc ccgtgaggcg gcaacccatg
     2521 cgactgatgc tgcaggctca gcacgcgcag ccagcacgtc agccggacag gccgcgtcgt
     2581 cggctcagtc agcgacttcc agcgcaggaa cggcatcaac aaaggccact gaagcatcaa
     2641 aaagtgctgc cgctgcagag tcctcaaaaa gcgcggcagc taccagtgcc agtgccgcga
     2701 aaacgtcaga aacgaatgct gcagcgtcac aacaatcagc agccacttct gcatccaccg
     2761 cgactacgaa agcgtcagaa gctgccacct cagcccggga tgcggcggct tcaaaagagg
     2821 cggcaaaatc atcagaaacg aacgcatcct cgagcgccag tagcgcagct tcctcggcaa
     2881 cagcggcagg aaattccgcg aaggcggcaa aaacgtcaga gacgaacgcc aggtcttctg
     2941 aaacggcagc gggacagagc gcctcggctg cggcaggctc aaaaacagcg gctgcgtcgt
     3001 ctgccagtgc agcgtcaaca agtgccgggc aggcctcagc cagtgccacc gccgccggaa
     3061 aatcggcaga aagcgccgca tcgtctgctt caacagccac aacgaaggct ggcgaagcca
     3121 ctgaacaggc cagcgcagca gcgaggtctg cttccgcagc gaagacatcc gaaacgaacg
     3181 cgaaagcgtc ggaaaccagc gcagaatcct caaaaacggc tgccgcatcg tcagccagtt
     3241 cggcgtcgtc atcggcctca tcggcgtctg cttcaaaaga tgaggcgacc agacaggcgt
     3301 cagcagcgaa gggtagcgcc acgacggcat ccacgaaggc gacagaggca gctggcagtg
     3361 cgacggcggc agctcagagc aaaagtacgg cggaatctgc agcaacgcgc gccgagacag
     3421 cggcaaaacg ggcagaggat attgcatccg ccgtggcgct tgaggatgcg agcacgacga
     3481 aaaaggggat agtacagctc agcagtgcga ccaacagcac ttccgagtca ctggcggcaa
     3541 cgccaaaagc cgttaaggcc gcgtatgacc tggctaacgg gaaatacacc gcacaggatg
     3601 caacgacagc acagaaaggg ataatccagc taagcagcgc gaccaacagc acgtctgaaa
     3661 cgctggcggc aacgccaaag gcagtaaaag cagccaatga caatgctgag aaacgtctgc
     3721 agaaagatca gaacggtgcg gatatccctg gcaaagacac ctttacgaaa aatattggtg
     3781 cctgccgtgc cttcggtggg tcagtaagca caacaacagg aaactggacg actgcacagt
     3841 ttatcgagtg gctggattct cagggagcat ttaaccatcc atactggatg tgcaagggtt
     3901 cctggtctta tggcaataat aaaatcatta ctgatactgg ctgcggtaat attcatctcg
     3961 ccggagctgt cattgaagta atggggataa agtcagcgat gacgatccgc attaccacac
     4021 cgaccacctc cactggtggt ggaacaacta acgcccagtt tacctatatt aatcacggaa
     4081 cagattattc acctggctgg cgaagggact ataactccag aaataagcca acggcatcag
     4141 agatcggggc gttaccgtca ggtggaacag cagtatcatc agttaatctg tcttcaaaag
     4201 gtcgggtaac cgcgctgaca gacaatacgc agggggcaac aggtcttgag ttatacgagg
     4261 tgtataacaa cggatatcca acagcgtatg gaaatatcat tcacctgaaa gggatgacag
     4321 ccgttggcga aggtgagtta ctcatcggct ggagtggtac aagcggtgct catgctccgg
     4381 catttattcg ttcacgacgg gatacgaccg acgcaaactg gtcgccgtgg gcgcagcttt
     4441 acacctcggc tcatcctcct gcagagtttt atccagtcgg tgcaccaatc ccgtggccat
     4501 cagataccgt tccgtctggt tatgccctga tgcaggggca gacttttgac aaatctgctt
     4561 acccgaaact tgcagccgct tatccgtcag gcgtgatccc tgatatgcgt ggctggacga
     4621 ttaagggcaa gcccgccagt gatcgagccg tattgtctca ggaacaggac ggcattaaat
     4681 cacacaccca cagcgccagc gtatccagta cggatttggg gacgaaaacc acatcgtcgt
     4741 ttgattacgg cactaaatcc acgaataaca ctggtgcgca tacccatagt gttagcggta
     4801 cggctgcttc agccggtgca catacccatt cgatgacatt tgtttcaggt ggttccagtg
     4861 gtgctccggg aagtggatca cctgattatt ctaaatacag tgttaacact tcttctgcag
     4921 gcgctcatac gcactctgta tcgggtactg ctgcaagcgc aggtgcacac gcacatactg
     4981 tcggtattgg tgctcatacg cactccgttg cgattggttc acatggacat accatcaccg
     5041 ttaacgctgc tggtaacgcg gaaaacaccg tcaaaaacat cgcatttaac tatattgtga
     5101 ggcttgcata atggcattca gaatgagtga acaaccacgg accataaaaa tttataatct
     5161 gctggccgga actaatgaat ttattggtga aggtgatgca tatattccgc ctcatacagg
     5221 tctgccagca aacagtaccg atattgcacc gccagatatt ccggcaggct tcgtggctgt
     5281 tttcaacagt gatgaggcat cgtggcatct cgttgaagac catcggggta aaacggttta
     5341 tgacgtagcg tcaggggacg cgttatttat ttctgaactc ggtccgttac cggaaaatgt
     5401 tacctggtta tcgccggaag gggagtttca gaagtggaac ggcacagcct gggtgaaaga
     5461 tgcagaagca gaaaaactgt tccggatccg ggaggcggaa gaaacaaaaa acagcctgat
     5521 gcaggtagcc agtgagcata ttgcgccact tcaggatgct gtagatctgg aaatcgcaac
     5581 ggaggaagaa acctcattgc tggaagcctg gaaaaaatat cgggtgttgc tgaaccgtgt
     5641 tgatacatca actgcacctg atattgagtg gcctacgaac cctgtcaggg agtaatcatt
     5701 gggattatgc cgcagcacgt cttaagcaag aacgtgctgc ggttggatgc tattttttcc
     5761 ctgaagcgga aaacattact acagtacctt gaaccttggt tttaacattc tcgaaatgct
     5821 ctgagagtat atgtgttaag ccttcttcgg aatcttttgt gtttgaaaag atgcctttct
     5881 gattgtaaat gcgcatcagt ttttgaccga agctattgtg cacaactcca tcgccaagaa
     5941 ttgtggctcc gtatagagtt ccatcgtcag ttaaggcctg cgccgcattg cgtattacac
     6001 agctttttgt agatatattt ccaggcaggc agtgaagaag gtaaaacatg gaaatggaat
//
LOCUS       DAJTNN010000208         6036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTNN010000208 DAJTNN010000000
VERSION     DAJTNN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.211"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(127..276)
                     /locus_tag="OO150_003729"
     CDS             complement(127..276)
                     /locus_tag="OO150_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517710.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="OO150_003730"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="OO150_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5517711.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="OO150_003731"
     CDS             2367..2669
                     /locus_tag="OO150_003731"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517712.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2845..4338)
                     /locus_tag="OO150_003732"
     CDS             complement(2845..4338)
                     /locus_tag="OO150_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR5517713.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4382..4771)
                     /locus_tag="OO150_003733"
     CDS             complement(4382..4771)
                     /locus_tag="OO150_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517714.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4989..5429
                     /locus_tag="OO150_003734"
     CDS             4989..5429
                     /locus_tag="OO150_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5517715.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1432 a         1663 c         1583 g         1358 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg tattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctggaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatgggttt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca ctagcggctc
     2761 cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca gcattttgat
     2821 tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct cagccggtgg
     2881 cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct tcaggtgatc
     2941 aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca tggcgccgcc
     3001 gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt
     3061 catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga ccttgttgcc
     3121 cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc
     3181 gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa
     3241 atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta
     3301 gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg ggcggctgat
     3361 gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg
     3421 caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac tggtcggcag
     3481 agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat
     3541 cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg ccagatagct
     3601 gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca cccggtgggc
     3661 gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc agcggaaacg
     3721 cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt ggaagtgaac
     3781 attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg tcttggccac
     3841 ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc
     3901 cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct gaaacgggaa
     3961 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4021 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4081 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4141 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4201 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4261 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4321 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4381 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4441 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4501 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4561 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4621 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4681 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4741 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     4801 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     4861 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     4921 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     4981 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5041 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5101 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5161 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5221 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5281 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5341 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5401 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5461 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5521 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5581 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5641 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5701 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     5761 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     5821 taagtgatgt atgaactgaa ggagcgatgt aattctgtag tcaggaagaa taacccgttc
     5881 atgcaggcat gaacgataca gtgaggaacg ctttttttgt cacgatctcc ccttaacctg
     5941 cgtaaatcac ccgccatggt tgattatttt tcaacctcaa aatggtcata cacctaacgc
     6001 agccgcgatt ttcatcgcaa ctgcatgaat taaaag
//
LOCUS       DAJTNN010000209         6026 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTNN010000209 DAJTNN010000000
VERSION     DAJTNN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.212"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..593
                     /gene="eutT"
                     /locus_tag="OO150_003735"
     CDS             <1..593
                     /gene="eutT"
                     /locus_tag="OO150_003735"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR5517716.1"
                     /translation="EHPQACCELCRQPVAKKPDTLTHLSAEKMVAKSDPRLGFRAVLD
                     STIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGCQAIVGLSDEDLHRLS
                     HQPLRYLDHDHLVLEASHGRDAALLNLLRTKVRETETVAAQVFITRSFEVLRPDILQA
                     LNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            583..1599
                     /gene="pta"
                     /locus_tag="OO150_003736"
     CDS             583..1599
                     /gene="pta"
                     /locus_tag="OO150_003736"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5517717.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSFIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YNIAQRLGGYRAVGPLIQGLAVPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            1638..1931
                     /gene="eutM"
                     /locus_tag="OO150_003737"
     CDS             1638..1931
                     /gene="eutM"
                     /locus_tag="OO150_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR5517718.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            2038..2325
                     /gene="eutN"
                     /locus_tag="OO150_003738"
     CDS             2038..2325
                     /gene="eutN"
                     /locus_tag="OO150_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR5517719.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            2337..3740
                     /locus_tag="OO150_003739"
     CDS             2337..3740
                     /locus_tag="OO150_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR5517720.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            3751..4587
                     /gene="eutJ"
                     /locus_tag="OO150_003740"
     CDS             3751..4587
                     /gene="eutJ"
                     /locus_tag="OO150_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR5517721.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVAELLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            4577..>6026
                     /locus_tag="OO150_003741"
     CDS             4577..>6026
                     /locus_tag="OO150_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR5517722.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVT
                     RLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSN
                     ADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAV
                     IGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAPGGLIFQS
                     ICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQV
                     TMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDC
                     CYTNHADADQNLNENLMILLATAGCNYIMGMPL"
BASE COUNT         1315 a         1614 c         1857 g         1240 t
ORIGIN      
        1 atgaacatcc gcaggcgtgc tgcgaactgt gccgccagcc ggtggcaaaa aagccggata
       61 cgctgactca cctgtcggcg gaaaaaatgg tcgccaaaag cgatccgcgt ctgggttttc
      121 gcgcggttct cgacagcacc attgcgctgg cagtgtggct gcaaattgaa ctggcggaac
      181 cgtggcagcc gtggctggcg gatatccgtt cgcgtcttgg caacattatg cgcgccgatg
      241 cgctggggga accgctgggc tgccaggcga ttgtcgggct ttctgacgaa gatctgcatc
      301 ggctttctca tcagccgctg cgctatctcg accacgatca tctggtgctg gaagccagcc
      361 acgggcgcga tgctgcgttg ctcaatctgc tacgcaccaa agtgcgggaa accgaaaccg
      421 tagcggcgca ggtctttatc acccgcagct ttgaagtatt acgcccggac attttgcagg
      481 cgctgaaccg cctttccagc acggtctacg tgatgatgat cctgagcgta acgaagcagc
      541 cgctaacggt gaagcagatc caacaacgac tgggggaacg caatgattat tgaacgttgt
      601 cgtgaactgg cgttgcgagc gcccgccaga gtggtttttc cggatgcgtt agatcaacgt
      661 gtgctgaaag ccgcgcaata tttacatcaa caaggtctgg caacgcccat tctggtcgcc
      721 aatccgtttg aacttcgtca gtttgcgctc agtcacggcg tggcgatgga cgggctacag
      781 gtgattgatc cgcatggcaa cctcgcaatg cgggaagaat ttgctcatcg ctggctggcc
      841 cgcgcgggcg aaaaaacgcc gccggatgcg ctggaaaaac ttaccgaccc tctgatgttc
      901 gccgcagcaa tggtcagcgc cggtaaagcg gatgtctgta tcgcaggcaa cctctcttcc
      961 acggcaaatg tgctgcgtgc cggattacgc attatcggct tgcagccagg ctgtaaaacg
     1021 ctctctttca ttttcctgat gttgccacag tacagcggcc cggcgttggg ctttgccgat
     1081 tgcagcgtgg tgccacagcc gacggcggcg cagctggcgg atatcgcgct tgccagtgcc
     1141 gaaacctggc gcgccatcac cggagaagag ccgcgcgtgg cgatgctgtc gttttccagc
     1201 aatggtagcg cccgtcaccc ctgtgttgct aacgtccagc aggcgacaga aatcgtccgt
     1261 gagcgcgcac caaagctggt ggtggatggc gagttacagt ttgacgccgc cttcgtgccg
     1321 gaagtggcgg cgcaaaaagc gcctgccagc ccgctacagg gcaaggccaa tgtgatggtt
     1381 tttccgtcgc tggaagccgg aaatattggt tacaacatcg cacaacgact cggcggatat
     1441 cgtgccgtcg ggccactgat acaaggactt gccgtgccga tgcacgatct ctctcgtggt
     1501 tgtagcgtgc aggaaattat cgagctggcg ctggtggcag ctgtgccgcg tcagacagaa
     1561 gtgaaccgcg aaagcagttt acaaacactg gttgaatgaa cggtcccgtt ctggaccctg
     1621 ataaagagga aagcacaatg gaagcattag gaatgatcga aacccggggc ctggttgcac
     1681 tgattgaggc ttctgacgcg atggtgaaag ctgcgcgcgt caagctggtt ggtgttaagc
     1741 agattggcgg cggcctgtgt actgccatgg ttcgtggtga tgttgcagcc tgtaaagccg
     1801 cgaccgatgc aggtgccgca gcggcacagc gtatcggcga gctggtatct gtacacgtta
     1861 tcccgcgtcc gcacggcgac ctggaagaag tcttcccgat cggcctgaaa ggcgatagca
     1921 gcaacctgta atcaccctgt gtgacgtcga ttcgggcgtt acgctctctc ctttttcatt
     1981 ttaccttctg cggagggtag ggggagcttt tacctgaaat acgccacgga ggcgggtatg
     2041 aaactggcag tcgtcactgg acaaattgtt tgtaccgtac gccatcacgg actggcgcat
     2101 gacaaattgc tgatggtgga aatgattgat ccacaaggta atcctgacgg gcaatgcgcc
     2161 gtcgccatcg acaatattgg cgcgggaacc ggggagtggg tgttactggt gagtggcagt
     2221 tccgcccgcc aggcgcataa aagcgaaacg tcaccggtcg atctgtgcgt gattggcatt
     2281 gtcgatgagg tggtgtctgg cggtcaggta attttccaca aataaggcag aacatcatga
     2341 atcaacagga tattgaacag gtggtgaaag cggtactgct gaaaatgcaa agcagtgaca
     2401 cgccgcccgc cgccgttcat gagatgggcg ttttcgcgtc cctggatgac gccgttgcgg
     2461 cagccaaagt cgcccagcaa gggttaaaaa gcgtggcaat gcgccagtta gccattgctg
     2521 ccattcgtga ggcaggcgaa aaacacgcca gagatttagc ggaacttgcc gtcagtgaaa
     2581 ccggcatggg ccgcgttgaa gataaatttg ccaaaaacgt cgctcaggcg cgcggcacgc
     2641 caggcgttga gtgtctctct ccgcaggtgc tgaccggcga caacggcctg actttaattg
     2701 aaaacgcgcc ctggggcgtg gtggcttcgg taactccgtc cactaacccg gcggcaaccg
     2761 taattaataa cgccatcagc ctgattgccg cgggcaacag cgtcattttt gccccgcatc
     2821 cggcggcgaa aaaagtctcc cagcgggcga tcacgctgct caaccaggcg attgttgccg
     2881 caggcgggcc ggaaaactta ctggttactg tggcaaatcc ggatatcgaa accgcgcagc
     2941 gtttgttcaa gtttccgggc atcggccttc tggtggtgac cggcggcgaa gcggtagtag
     3001 aagcggcgcg taaacacacc aataaacgtc tgattgccgc aggcgctggc aacccgccgg
     3061 tagtggtgga tgaaaccgcc gacctcgccc gtgccgctca gtccatcgtc aaaggcgctt
     3121 ctttcgataa caacatcatt tgtgccgacg aaaaggtact gattgttgtt gatagcgtag
     3181 ccgatgaact gatgcgtctg atggaaggcc agcacgcggt gaaactgacc gcagaacagg
     3241 cgcagcagct gcaaccggtg ttgctgaaaa atatcgacga gcgcggaaaa ggcaccgtca
     3301 gccgtgactg ggttggtcgc gatgcggcga aaatcgcggc ggcaatcggc ctgaacgtgc
     3361 cgcaagaaac gcgtttgctg tttgtggaaa ccactgcaga acatccgttt gccgtgaccg
     3421 aactgatgat gccggtgctg cccgtcgtgc gcgtcgccaa cgtggcggat gccattgcgc
     3481 tggcggtgaa actggaaggc ggttgccacc acacggcggc aatgcactcg cgcaacatcg
     3541 aaaacatgaa ccagatggcg aatgctattg ataccagcat tttcgttaag aacggaccgt
     3601 gcattgccgg gctggggctg ggcggggaag gctggaccac catgaccatc accacgccaa
     3661 ccggtgaagg ggtaaccagc gcgcgtacgt ttgtccgtct gcgtcgctgt gtattagtcg
     3721 atgcgtttcg cattgtttaa ggagtaacag atggcgcacg acgaacaatg gctcacccca
     3781 cgtctgcaaa cggcggcaac gctgtgtaac cagacgcccg ccgcgacgga atctccgctg
     3841 tggctggggg tggatctggg cacctgcgat gtggtgtcga tggttgtcga ccgcgacggt
     3901 cagccggtgg cggtatgcct cgactgggcc gatgtggtcc gcgacggcat cgtctgggat
     3961 ttcttcggcg ctgtcaccat tgttcgtcgc catctcgaca cgctcgagca gcaattcggt
     4021 cgccgtttta gccatgcggc gacttcattt cctccaggta cggacccgcg tatttccatc
     4081 aacgtgctgg agtctgctgg actggaagtg agccatgtgc tggatgagcc aaccgcagtt
     4141 gcggagctgc tgcaactgga caacgccggt gtagtggata tcggcggcgg cactaccggc
     4201 attgccatcg tgaaaaaggg caaggtgacg tactcggcgg atgaagcaac cggcggacat
     4261 cacatctctc tgactctcgc cggaaatcgc cgtatttcgc tggaagaggc ggagcagtac
     4321 aagcgcggtc acggtgaaga gatttggcct acggtgaaac cggtgtacga aaaaatggcg
     4381 gacattgtcg cccggcatat tgaagggcag ggcattactg atttatggct ggcgggcggc
     4441 tcttgtatgc aaccgggcgt ggcggaactg tttcgcaaac aattcccggc gttacaggtg
     4501 catttaccgc agcacagttt gtttatgacc ccgctggcga tcgccagtag tgggcgagag
     4561 aaagcggagg ggctatatgc aaaatgaatt gcagaccgcg ctctttcagg cgttcgatac
     4621 cctgaatctg caacgggtaa aaacatttag cgttccaccg gtgacgcttt gcggtccggg
     4681 ctcggtgagc agttgcggac agcaagcgca aacgcgtggg ctgaaacatc tgttcgtgat
     4741 ggcagacagc tttttgcatc aggcagggat gaccgccggg ctgacgcgca gcctggctgt
     4801 taaaggcatc gccatgacgc tctggccatg tccggtgggc gaaccgtgca tcaccgacgt
     4861 gtgtgcagcc gtggcgcagt tgcgtgagtc aggctgtgat ggggtgatcg catttggcgg
     4921 cggctcggtg ctggatgcgg cgaaagccgt ggcgttgctg gtgacgaacc cggatagcac
     4981 gctggcagag atgtcagaaa ccagcgttct gcaaccgcgc ttgccgctga ttgcctatga
     5041 agatgactgc gtgacgcggc tgattcagga cgatgttaac gaaacggcct acaaccagat
     5101 taaaaactgg agcatcagcg aactgcgtga gtatgtgctg agcgatgaaa ctagcgtgga
     5161 cgacattgcc tttacccgca aagggctgac ctcggaagtg gtcgcggcgg tagcgaagat
     5221 ttgctccaac gcggacctga tctatggcgc gaagaaaatg ccggtaatca aaaaggccaa
     5281 taccaccatc ggtattccgg gcaccttcag cgcccgtttg cagccaaatg acactcgtga
     5341 cgacgtgcaa agtatcgccg cgcaaatcta cgaagggctt tccttcgggg tgggcgatgc
     5401 ggtgatcggc gtcaacccgg taactgacga cgtggaaaac ttaagccgcg tgctggatac
     5461 catttatggc gtgatcgaca aattcaacat cccaactcag ggctgcgtac tggcgcacgt
     5521 caccacccag atcgaagcga ttcgtcgcgg cgcgcctggc ggactgattt tccagagtat
     5581 ctgtggcagc gaaaaagggc tgaaagagtt tggcgtggaa ctggcgatgc tcgacgaagc
     5641 gcgcgcagtg ggtgcagagt tcaaccgtat cgccggggaa aactgcctct acttcgaaac
     5701 cggacaaggc tctgcgctat ccgctggcgc taacttcggc gctgaccagg tgacgatgga
     5761 agcacgtaac tatgggctgg cgcgtcatta cgatccgttt atcgtcaaca ccgtggttgg
     5821 ctttattggg ccggagtatc tctacaacga ccgccagatt atccgtgctg gcttagaaga
     5881 tcactttatg ggcaagctga gcggcatctc tatgggctgt gactgctgct acaccaacca
     5941 cgctgacgct gaccagaacc tcaacgaaaa cctgatgatc ctgctcgcca ccgcaggctg
     6001 caactacatc atggggatgc cgctgg
//
LOCUS       DAJTNN010000210         5990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTNN010000210 DAJTNN010000000
VERSION     DAJTNN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.213"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..157
                     /locus_tag="OO150_003742"
     CDS             <1..157
                     /locus_tag="OO150_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5517723.1"
                     /translation="ILQLCRNDSLMIGRESFSVDRITPDDGGCSYIRLRREGAGNVRA
                     GGYYEGA"
     gene            144..731
                     /locus_tag="OO150_003743"
     CDS             144..731
                     /locus_tag="OO150_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5517724.1"
                     /translation="MRGLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            728..1129
                     /locus_tag="OO150_003744"
     CDS             728..1129
                     /locus_tag="OO150_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5517725.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1140..1880
                     /locus_tag="OO150_003745"
     CDS             1140..1880
                     /locus_tag="OO150_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5517726.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1939..2325
                     /locus_tag="OO150_003746"
                     /pseudo
     CDS             1939..2325
                     /locus_tag="OO150_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
     gene            2334..2662
                     /locus_tag="OO150_003747"
                     /pseudo
     CDS             2334..2662
                     /locus_tag="OO150_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            2634..5675
                     /locus_tag="OO150_003748"
     CDS             2634..5675
                     /locus_tag="OO150_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5517727.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALVEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAVGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5675..>5990
                     /locus_tag="OO150_003749"
     CDS             5675..>5990
                     /locus_tag="OO150_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5517728.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVACRKWSASVGALWVTVTA
                     DFE"
BASE COUNT         1452 a         1369 c         1942 g         1227 t
ORIGIN      
        1 tattttacag ctgtgtcgca atgattcgct gatgattggc cgggagtctt tcagcgtgga
       61 tcgtattact cctgatgacg gcggatgtag ttatatccgg ttgcggcgtg aaggagcagg
      121 aaacgtaagg gcagggggat attatgaggg ggcttgagaa tgccatccgc aatctgaaca
      181 gtcttgatac ccgtatggtg ccgcaggcca gcgcatgggc agtaaaccgg gttgctgcaa
      241 aagttgtctc ggttgccacc cggcaggtcg ccaggaatac cgttgcggga gataaccagg
      301 tgaagggggg ccccctgaaa ctggtgcgtc agcgcgtccg ggtatttaaa gccagtccgg
      361 caggaaaaat gtatgcccgt atccgtgtta accggggcaa tctgcccgcc attaagcttg
      421 gcacaacatg ccggcgtgcc ggtgacggac tgaaggtggg gaaatacttt tttcgtggtg
      481 cgtttatcca gcagctggcg aacggtcgct ggcatgttct caggcgtctt cctgaagcgt
      541 gttttgaaac aggaaaggac agtaaggggc ggcccagaaa aaatcgtctg cccgtggagg
      601 ttgtgaaaat tccgctggcc ggaccgctga cacaggcatt tgaggatgcc cgcgaccgca
      661 tcattgccgc ggaaatgccg aaacagctgg ggtatgccct gaaacagcaa ctgaggatac
      721 atttgggatg aacaggcaca cacaaatccg ccaggcagta ctggcaagcc tgaagggcga
      781 atgcgggggg aatgtcgtgc tttttgacgg gcttccggca tttattgatg cgcaggaact
      841 gcccgccgtg gcggtgtggc tgagtgatgc gcagtacacc ggaaaaatga cggatgagga
      901 cgactggcag gctgttctgc atattgccgt tttcgtccgt gcacaggcgc cggattctga
      961 gctggatacg tggatggaga acaccatatt tccggccatg aaggatattc cggtgctttc
     1021 cggactcatc gacaccatga tcccactcgg ttttaactat caacgtgata atgagatggc
     1081 cacctgggcg atggcggaaa tcacgtacca gatcacgtac acgaattaag gaggtggcaa
     1141 tgaccacacc aaatccactg gcaaagacga aaggtgcggg aacgacgttc tggatgtaca
     1201 ccggcaaggg cgatgcgttt gcgaaccctt tgtcggacac tgactggctg cgtcttgcga
     1261 tggtgaagga tctgcaacct ggcgaaatga ccgctgatgc agaagatgac acttatctcg
     1321 atgatgaaga tgcagactgg aaaacgacaa cccaggggca gaaatccgtc ggtgatactt
     1381 tggcgacgct ggcctggcgt ccgggtgaca gcgggcagaa aaaactggtt cagttgttcg
     1441 attccggtga agtctgcgcg tttcgtatca aataccccaa cggtacggtt gatgttttcc
     1501 gtggctggct gagttcactg ggtaaaacca ttacctcaaa agaggtgatg acccgtactg
     1561 taaaaatcag cggtgtgggg cgtccgtatc tggcagagga gggcgctgaa accgtgggtg
     1621 ttaccggact gacggtggca ccggcatctg tcagtgtcaa agcgggagca accaccacac
     1681 tgacctttac agtaaaacct gacggggcca gtgacaaagc gatcagtgtg cattcgtcag
     1741 atcctcagac tgcttcggtg accctgagcg ggcttgtggc cacggtgaaa ggcgtgaagc
     1801 agggcagtgt cagcatcgtg ggcatgacct ctgacggaga gtttgtggca gtggctgcgg
     1861 tgaccgtcag cgcaccataa caggacgata ctcatcattg ccccggttat ccggggcttt
     1921 ttgcatccgg agaacatgat gtttctgaaa caggatacgt ttaattatga aaaacagtcc
     1981 gtggtgctca gtgagctgtc cgggctgcag agaattgaat atctgacgtt tgttcagcag
     2041 cgaacggcaa agtttgatgc acaggaggga gaactgccgg aggctgaacg acagattgct
     2101 tttctgcgta tgggaatgga tatcaatgcc tggctggttt cccgctcact gtggaatgct
     2161 gagcagtctc aggatgttga gacgctttga gcatccatta tgacaacatg gtcgtatgat
     2221 gcgctgggcg cgggggcgga gagggttctg tcgctgagcg gtatggggac cattgagaat
     2281 gccggggatg atgatcatga ggcgctgacg ccggaaaagt cctgacgcgg gaaatgcagt
     2341 ttgtcatgcg gcttgcccgg gagttccggc gggcagactg gcggcggatg ctgtcggaaa
     2401 tgtcggccac tgagcttggt gagggggcga ttatttccgg atgcagagct tcagtgatgt
     2461 gtggatggat gcgcagtttg cctcgctgaa ggcattgatc gtgagaatgg tgtccggcag
     2521 cagtgatgct gcggtggctg atttcagcct tttaccggaa gagaacggga taccggagcg
     2581 aacggacgaa gaactgatgc atcttgggga aggtatttcc ggaggtgtgc gttatggacc
     2641 agatagccaa cctggtcatt gatttgggga ttgatgcggc agagtttaaa aatgaaattc
     2701 cccgtatcaa aaaccttctg aatggtgcag ccggtgatgc agaacggtct tctgcccgta
     2761 tgcagcgttt tatggagcgt cagactcagg cggcccggca gacaatgcat gcggcttctt
     2821 cggctgcaac agcagcatca gcccatgcgc agacggtgga gaagaatgca cgggctcatg
     2881 aacgcatggc ccgtgaggtg gaacaaaccc gtctgcgcgt ggatgccctg aatcagaaaa
     2941 tgcgcgagga acaggcgcag gccagggcac tggtggaggc gcaggataaa gcggctgccg
     3001 ccttttatcg ccagattgac agtgtgaaac aggccggtgc ggggcttcag gaattacagc
     3061 gtattcagca gcagatccga caggccagaa acagtggcgg ggttggtcag caggattatc
     3121 tggcgctgat ttcggagatc acggcgaaaa cccgtgccct gacgcaggca gaggaacagg
     3181 ccacccggca gaaagcggcg tttatccgcc agcttaaaga gcaggcaacc cgccagaatc
     3241 tgtcgtcttc tgagttgctt cgcgccaggg cggctcagct gggggtaagc agtgctgcag
     3301 aagtgtatat ccgcaaaatg gagcgggcag gaaaagccac acattcgctg gggctgaaaa
     3361 gtgcggcagc ccggcgggag ctgggggtgt taatcagtca gatggcgcgc ggcaattttg
     3421 gtgcgctgag gggatccggg ataacgctgg ctaaccgtgc cggatggaca ggcgcactga
     3481 tgtcgccgaa aggcatgatg actggcggcg ttattggcgg acttgtcgcg gcggtcctgg
     3541 gtctgggtaa agcctggcat gacggccgga aggagggcga ggaatttaac cgtcagctgg
     3601 cgctgacggg acattatgcc ggtgtcactg tcgggcagtt gtggaaactc agccgggcca
     3661 tatccgggaa tggtatcacg caacatgcgg cagccggtgc gctggcacag gtggtgggca
     3721 gtggggcatt tcatggaaac gatattggta tggtggcgaa agctgccgca cagatggagc
     3781 gatcggttgg ccagtcggtc agcgatacca taaatcagtt taagcggctg aaggatgatc
     3841 ctgtaaatgc cgcgaaggcg ctggacaatg cgctgcattt tttgactgcc actcagcttg
     3901 agcagatacg cgtccttggg gagcaggggc ggtccagtga tgcggcccgg atcgccatgt
     3961 ctgcgctggc agaggaaacc ggtaaacgca catctgatat tgataataat ctcaatgcgc
     4021 tgggtagtac gctgcaaacc ttgtctgact ggtggaagca gttctgggat gcggccatga
     4081 atattggtcg tgaagactcg ctggatgcgc agattgatgc gttacaggaa aaaattcagc
     4141 gcgcgaaaaa atatccgtgg acaaacgcct ccacacaggt ggagtacgat cagcagcgtc
     4201 ttaacgatct tcaggagaaa aaacgccgga aggatttgca ggatgcaaaa gcgcaggcag
     4261 aacggaatta ccaggagcaa cagaaacgcc ggaatgctga aaatgccgcg ctgaaccgga
     4321 tgaatgaaac ggaagctgca cgacatcagc gggaaattgc gcgtattaat gccatgcagt
     4381 acgccgacca ggctgtcagg gatgcggcga tacagcgtga aaatgaacgt tacgaaaaag
     4441 ccattaagaa aaatacacgg gcaacccgta atgatgaggc cacccggtta ttgctgcagt
     4501 acagtcagca gcaggcacag gtggaagggc agattgctgc tgccagacag tcagcaggca
     4561 ttgccactga aaggatgact gaagcgcata aacagcttct ggccctgcag cagcgcatca
     4621 gcgacctgga cgggaaaaag ctgacggcag atgaaaagag tgtgctggcc cgtaagaatg
     4681 agctgattca ggcgctgacg ctgctggatg tgaaacagca ggagctgcag aaacagacag
     4741 cgcttaacga cctgagaaaa aaaacggttc agctgaccag ccagctggca gacaaagaac
     4801 gtgcactgcg tgagcagcac aatctggata ttgccactgc aggtatgggg gataagcagc
     4861 ggcagcgcta ccaggcacag ttgcgcatcc ggcaggaata ccggcaacag ttgcaacagc
     4921 ttgagaatga cagtcgccag aaaggcactt acgggacgga ggactaccgg agggctgagg
     4981 aggtgctgaa ggggagcctg aagcgacaac tgaatgaaaa caaacgctac tggcaggaac
     5041 tggaagtggc gcagggcgac tggaaaaacg gtgccatgcg ggcgtttcag aattttacgg
     5101 cggatgcgga taatgcggtg ggaacggcag aacagatgtt tacagtggca ttcagcagtg
     5161 ccggtaatgc actggcgaca ttctgtacca ccggtaagct gaattttaaa tccttcacct
     5221 cttccctgtt gtcagatatg gccagaatta tggcacagat ggccatgatg caggcggtaa
     5281 agggcgtcgg ctctttattc ggcttcacga ctaatgctga tggcggtgtt taccagtctg
     5341 ctgatttgag tcgctacagt ggcacggtgg ttaaccgtcc gacgtttttt gcttttgcaa
     5401 aaggcgcggg tgtgatgggg gaagcgggac ctgaagccat tctgccactg cgtcgcggtg
     5461 ctgatggtaa gctgggggtt gtggcggata ctggtggttc aggcatggtg atgtttgccc
     5521 cgcagtacaa catcgggatc aataacgacg gcacgaacgg gcagataggt ccggcagcac
     5581 tgaaggtggt ttatgacctc gggaaaaaag cggccgcgga ctttatgcaa cagcaggccc
     5641 gtgatggtgg ccggttaagt ggagcatacc ggtaatggag acgtttcgct ggaaggtgcg
     5701 cccggatatg aatgtggtat cagagccaaa agtggtggca gtgaagctgg gtgatggtta
     5761 tgaacagcgt cgcccggcgg gactgaataa tctgctgtcg acttacagcg tgacgatacg
     5821 tgttcgtaaa ggtgaacacc cacctttaaa agcctttctg gaacggcacg gtggcgtccg
     5881 cgcatttcag tggacgccac cttatgactg gaaaccgatc agggtggctt gtcgtaaatg
     5941 gtcggcaagc gtgggggcgt tatgggtgac tgtaacggcc gattttgaac
//
LOCUS       DAJTNN010000211         5912 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTNN010000211 DAJTNN010000000
VERSION     DAJTNN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.214"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..406)
                     /gene="dsbG"
                     /locus_tag="OO150_003750"
     CDS             complement(<1..406)
                     /gene="dsbG"
                     /locus_tag="OO150_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5517729.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQ"
     gene            778..1341
                     /gene="ahpC"
                     /locus_tag="OO150_003751"
     CDS             778..1341
                     /gene="ahpC"
                     /locus_tag="OO150_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5517730.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1512..3077
                     /gene="ahpF"
                     /locus_tag="OO150_003752"
     CDS             1512..3077
                     /gene="ahpF"
                     /locus_tag="OO150_003752"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5517731.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3198..3626)
                     /gene="uspG"
                     /locus_tag="OO150_003753"
     CDS             complement(3198..3626)
                     /gene="uspG"
                     /locus_tag="OO150_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5517732.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLDRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            3847..5085
                     /locus_tag="OO150_003754"
     CDS             3847..5085
                     /locus_tag="OO150_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR5517733.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEEAQKAVSGLVNAMPGGTI"
     gene            complement(5316..5726)
                     /gene="rnk"
                     /locus_tag="OO150_003755"
     CDS             complement(5316..5726)
                     /gene="rnk"
                     /locus_tag="OO150_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5517734.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
BASE COUNT         1525 a         1408 c         1533 g         1446 t
ORIGIN      
        1 cctgctgcca gaactgttta caatatgggc agaacggatc ggcgaagacg tagacaatga
       61 ccggcgcatc ttttttaccg tcgaggagcc agtgggattg ttccatccgt tgccacattt
      121 cgcgtccggc tggtgcgtaa atttcttttt cgataagtgt gttactcagg ttttcacctt
      181 tctcgttgta catgtaacca gagatagcgt gcttaccatc tggagtcagg tagatggtga
      241 cgcccatatc ctgatacttt ccgagataac ctttcattcc tccgggggca tcgaacgttt
      301 tgatgattgt aatgccctgt ttttcaatcg cttttactgg agcaggaagt tcctctgcga
      361 aggcgattgc aggaagcaga gccagtaaaa gtatcttttt taacattaat ttgtcctttt
      421 cagtcagtgc aaaagtcgag taaaaggcat aacctatcac tgtcataggt aagagcttag
      481 atcaggtgat tgccctttgt ttatgagggt gttgtaatcc atgtcgttgt tgcatttgta
      541 agggcaacgc ctcagcctgc aggcaggcgc tgaagatacc aaagggtagt tcagattaca
      601 cggtcacctg gaaagggggc cattttactt tttatcgccg ctggcggtgc aaagttcaca
      661 aagttgtctt acgaaggttg taaggtaaaa cttatcgatt tgataatgga aacgcattag
      721 ccgaatcggc aaaaattggt taccttacat ctcatcgaaa acacggagga agtatagatg
      781 tccttgatta acaccaaaat taaacctttt aaaaaccagg cattcaaaaa cggcgaattc
      841 atcgaaatca ccgaaaaaga taccgaaggc cgctggagcg tcttcttctt ctacccggct
      901 gactttactt tcgtatgccc gaccgaactg ggtgacgttg ctgaccacta cgaagaactg
      961 cagaaactgg gcgtagacgt atacgcagta tctaccgata ctcacttcac ccacaaagca
     1021 tggcacagca gctctgaaac catcgctaaa atcaaatatg cgatgatcgg cgacccgact
     1081 ggcgccctga cccgtaactt cgacaacatg cgtgaagatg aaggtctggc tgaccgtgcg
     1141 accttcgttg ttgacccgca gggtatcatc caggcaatcg aagttaccgc tgaaggcatt
     1201 ggccgtgacg cgtctgacct gctgcgtaaa atcaaagcag cacagtacgt agcttctcac
     1261 ccaggtgaag tttgcccggc taaatggaaa gaaggtgaag caactctggc tccgtctctg
     1321 gacctggttg gtaaaatcta aatttccttc gtctttcgcg ctaccaactc cggcgcgaaa
     1381 tacttcggaa attcacctaa ttcttcgggt gctgcggcac ccgattttct tccccgcact
     1441 atgatgcaag ctgcatccag gtagccgtag aggccgcttg catgatgatg tttaaagccc
     1501 aggagataaa catgctcgac acaaatatga aaactcaact caaggcttac cttgagaaat
     1561 tgaccaagcc tgttgagtta attgccacgc tggatgacag cgctaaatcg gcagaaatca
     1621 aggaactgtt ggctgaaatc gcagaactgt cagacaaagt cacctttaaa gaagataaca
     1681 gcttgccggt gcgtaagccg tctttcctga tcaccaaccc aggttccaac caggggccac
     1741 gttttgcagg ctccccgctg ggccacgagt tcacctcgct ggtactggcg ttgctgtgga
     1801 ccggtggtca tccgtcgaaa gaagcgcagt ctctgctgga gcagattcgc catattgacg
     1861 gtgattttga attcgaaacc tattactcgc tctcttgcca taactgcccg gacgtggtgc
     1921 aggcgctgaa cctgatgagc gtactgaacc cgcgcatcaa gcacactgca attgacggcg
     1981 gcaccttcca gaacgaaatc accgatcgca acgtgatggg cgttccggca gtgttcgtaa
     2041 acgggaaaga gtttggtcag ggccgcatga cgttgactga aatcgttgcc aaaattgata
     2101 cgggcgcgga aaaacgtgcg gcagaagagc tgaacaagcg tgatgcttat gacgtattaa
     2161 tcgtcggttc cggcccggcg ggtgcagcgg cagcaattta ctccgcacgt aaaggcatcc
     2221 gtaccggtct gatgggcgaa cgttttggtg gtcagatcct cgataccgtt gatatcgaaa
     2281 actacatttc tgtaccgaag actgaagggc agaagctggc aggcgcactg aaagttcacg
     2341 ttgatgaata cgacgttgat gtgatcgaca gccagagcgc cagcaaactg atcccagcag
     2401 cagttgaagg tggtctgcat cagattgaaa cagcttctgg cgcggtactg aaagcacgca
     2461 gcattatcgt ggcgaccggt gcaaaatggc gcaacatgaa cgttccgggc gaagatcagt
     2521 atcgcaccaa aggcgtgacc tactgcccgc actgcgacgg cccgctgttt aaaggtaaac
     2581 gcgtagcggt tatcggcggc ggtaactccg gcgtggaagc ggcaattgac ctggcgggta
     2641 tcgttgagca cgtaacgctg ctggaatttg cgccagaaat gaaagccgac caggttctgc
     2701 aggacaaact gcgcagcctg aaaaacgtcg acattattct gaatgcgcaa accacggaag
     2761 tgaaaggcga cggcagcaaa gtcgttggtc tggaatatcg agatcgtgtc agcggcgata
     2821 ttcacaacat cgaactggcc ggtattttcg tgcagattgg tctgctgccg aacaccaact
     2881 ggctcgaagg cgcagtcgaa cgtaaccgca tgggtgagat tatcattgat gcgaaatgcg
     2941 aaaccaacgt caaaggcgta ttcgcagcgg gtgactgtac gacggttccg tacaagcaga
     3001 tcatcatcgc cactggcgaa ggtgccaaag cctctctgag tgcttttgac tacctgattc
     3061 gcaccaaaac tgcataagaa gaagtaagat tcacctgcaa ttgcttagcc gccggggtca
     3121 aacctagcgg cttttttatg gcattaaaaa gccctgccgg gatgttccag gcagggcggc
     3181 ataaatactt ttatctgtta gcgaacgacc agtacaggca gattggcgtg gcggatcacg
     3241 ctcgaggcgt tagaacctaa cagatgggtc gaaatcgatg ggttgcgaga accgataact
     3301 acaacatcag cccccagttc ttctgccaac tcattgactt catcccgcac gctaccaaaa
     3361 cggacatgtt gtttaatgcg ggaaggatcg atggtgaagt ggctgaccat cgtttgcaga
     3421 cgttcttctg cttcatgttg cagatgctct tcaaaacgac gcacatcagc ggcaaaacgg
     3481 tccaggctca ggctggctga cccgggtagt acgtgaagta gatgaataac tccgtcatcc
     3541 tgggtgagga attcagcgtg gcgaacagct ttgtcgctca attccatttc aaatacatca
     3601 actggcataa tgattgtctt atacataacc ctttctccct gttaatcatg aacaaatcat
     3661 tcgccatgat tataatattt atccctgata tttgtctggt tctttttcct tacgaacggt
     3721 ttctgtgatt aatatattct cactgaacac caggaattct cccaaaacct gcggtaccgc
     3781 ccgttttccc gctgtgatag ctacccttaa agactgactc ttttttgaac tgtctctgga
     3841 ggttgcatga aagcattgac ttatcacggc ccacatcacg ttcaggtaga aaatgttccc
     3901 gatccgggca ttgaacaggc agatgatatt attctgcgta ttacggcaac ggcgatctgt
     3961 ggctctgatc tccatcttta tcgaggcaaa ataccccagg ttaaacatgg cgatattttt
     4021 ggtcatgaat ttatggggga agtcgttgaa accggaaagg acgtaaaaaa tttgcaaaaa
     4081 ggcgaccggg tggtaattcc gtttgtcatt gcttgtggcg actgtttttt ctgtcgaatg
     4141 cagcaatatg ccgcctgcga aaataccaat gcgggtaaag gcgctgcgct caataaaaaa
     4201 cagataccag ctcccgcggc attgtttggt tatagtcacc tgtatggtgg cgttcctggt
     4261 ggacaggcgg aatatgtccg cgtccctaaa gggaatgtgg ggccgtttaa agtaccgcct
     4321 ttgctttcag atgataaagc ccttttcctt tctgatattc tgccaacggc atggcaggca
     4381 gcaaaaaatg cgcagatcca acaaggttca agcgttgcag tctatggtgc cggtccagtg
     4441 ggattgttga caatcgcctg tgcacggttg ctcggtgcgg aacagatttt tgttgttgat
     4501 catcatccct accgcttgag tttcgccgct gaccgctacg gcgcgatccc gattaatttc
     4561 gatgaagata gcgatccggc acagtcaatt attgaacaaa cggcaggtca ccggggcgtg
     4621 gatgcagtaa tagacgccgt cggttttgaa gcgaaaggca gcaccacgga aacggtgctg
     4681 actaacctga aactggaggg cagcagcggt aaagcgttgc gccagtgtat tgcggcggtc
     4741 aggcgtggcg gcattgttag cgtaccgggc gtctacgctg gatttattca cggtttcctg
     4801 tttggtgacg cctttgataa agggttgacg tttaaaatgg gacagaccca cgttcacgca
     4861 tggctgggag aattattacc gttaattgag aaaggattac tgaaaccaga agaaattgtt
     4921 acccactata tgccgtttga agaggccgcc cggggatatg agattttcga aaaacgtgaa
     4981 gaggagtgcc gtaaggtgat tctggtaccc ggtgcacaaa gcgcagagga ggcgcagaag
     5041 gcagtttcag gtctggtgaa tgcgatgccg gggggaacaa tatgatcgtc aggagtggtt
     5101 ttcgaggtaa aggacagcca tgacgataat cgccgccata atcagaaatc ctatcaggat
     5161 gtaaaatgct tctgccatgg ttattcccac aaacgaaacg gaataatttt gcagcaaaag
     5221 tgaacagtga gaaccaggaa aaattgctga ttttgcgtaa agaggatgcg agtgcatcct
     5281 ctgggcaaag cgagttatcg cttgtgctga tgggattaaa gcaggtagtc gccagcagct
     5341 tctggctggt attcgagttc cagcacttca aggtgggttg caacgccgcc cggaagttcc
     5401 cagtgaatgg aatcgccaac gcgcagcccc agcagtgcgg cacctaccgg agccataacg
     5461 gaaagctgag tattgctatc ggtcattttt gccggataca ccagcgtgcg cacacgcact
     5521 tcgccatcgc taagattgcg gaatttaacc cggctgttca ttgtcaccac gtcgtgtggc
     5581 atctcttctg gcgaacacat ttgggcgcga tccaactctg cgtttaacgc gtcggcgatt
     5641 ggcaaaccag cataggcggg ttgctccagc agaatatcga tgcgttcggc atccaggtcg
     5701 ttaatgatga tagttggtct ggacattttt actccatgtc gtcggtactg cgagtgtcgc
     5761 agataaacat acccaaaaga aaaccctcac cgtcaggcgg cgagggttta actcacatga
     5821 tgatactgac tgttgctcac tctttgaagt gatttgcgtc acattcaggg aattcctcaa
     5881 tgcagcgcat tatgtataat tcttaatcgt ca
//
LOCUS       DAJTNN010000212         5908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTNN010000212 DAJTNN010000000
VERSION     DAJTNN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.215"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..308
                     /locus_tag="OO150_003756"
     CDS             <1..308
                     /locus_tag="OO150_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HCR5517735.1"
                     /translation="RKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRL
                     KGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            305..568
                     /locus_tag="OO150_003757"
     CDS             305..568
                     /locus_tag="OO150_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5517736.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            561..947
                     /locus_tag="OO150_003758"
     CDS             561..947
                     /locus_tag="OO150_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5517737.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            955..1641
                     /locus_tag="OO150_003759"
     CDS             955..1641
                     /locus_tag="OO150_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5517738.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1619..2242)
                     /gene="tetR(B)"
                     /locus_tag="OO150_003760"
     CDS             complement(1619..2242)
                     /gene="tetR(B)"
                     /locus_tag="OO150_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5517739.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2324..3529
                     /gene="tet(B)"
                     /locus_tag="OO150_003761"
     CDS             2324..3529
                     /gene="tet(B)"
                     /locus_tag="OO150_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5517740.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3642..4235)
                     /gene="tetC"
                     /locus_tag="OO150_003762"
     CDS             complement(3642..4235)
                     /gene="tetC"
                     /locus_tag="OO150_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5517741.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4368..4739
                     /locus_tag="OO150_003763"
     CDS             4368..4739
                     /locus_tag="OO150_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5517742.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELKLTKKTILEIALQYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4749..>5908)
                     /locus_tag="OO150_003764"
     CDS             complement(4749..>5908)
                     /locus_tag="OO150_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5517743.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1624 a         1106 c         1310 g         1868 t
ORIGIN      
        1 tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc
       61 ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc tggccgctgt
      121 atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg cagcgaattg
      181 agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag tcgctgtcgt
      241 tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact
      301 atcaataagt tggagtcatt accgaccact attaagtcat gtagctggtt ttatttcaat
      361 tgaacgtttt caaagtctag ctacagaagg aaaaatgtta tcgctatctt ggtgggaaaa
      421 cgaatacgca gttctgcaat ggaaaaatca tgttttacat gcgaaagctc aacaagaagg
      481 gcgagagtca atatttgatt tttacaaaat tagtattgct catattactc gcgaatattc
      541 atttaaaaag gacaaggata atgtttgatg ttcacgttgt tttagataat caaataggac
      601 aattagcatt actaggaaaa acattaggta ataaaggtat tggattggaa gggggaggga
      661 tatttacggt tggtgatgaa tgccatgctc attttcttgt tgaacaagga aaggaagcta
      721 aaatagcgct agagcaagct ggactgttag tacttgcgat ccggacacca ttaattcgta
      781 agttaaaaca ggaaaaaccg ggggaacttg gcgaaatagc acgagtattg gcggagaata
      841 acattaatat tttagtgcaa tacagtgacc atgctaacca actgatatta ataacggaca
      901 atgatagtat ggctgcatct gttacgctcc cttgggcaat aaagtgaact tgcgatggct
      961 aatttaatac gaaaagaggt tacctttgag tcctcaatag ccgcgatagg ggcggctatg
     1021 tctgacattt cacgagttaa aatactcagt gctttgatgg atgggcgagc ttggacggcc
     1081 actgagctaa gttctgtggc gaatatatca gcttcaacgg cgagcagtca tttatctaaa
     1141 ttattagatt gccagctaat cacagtagta gctcaaggca agcatcgtta ttttcggcta
     1201 gcaggaaaag atattgctga attgatggaa agtatgatgg ggatctcctt aaaccatggc
     1261 gtacatgcca aagtttccac gccagtgcat ttacgaaaag cacgtacttg ctatgatcat
     1321 ttagctggcg aagttgccgt taagatctat gattcccttt gtcaacagca atggatcact
     1381 gaaaatggtt caatgatcac attaagtggt attcaatatt ttcatgaaat gggaattgac
     1441 gttccttcca aacattcacg taaaatctgt tgtgcgtgtt tagattggag tgaacgccgt
     1501 ttccatttag gtgggtacgt tggagccgca ttattttcgc tttatgaatc taaagggtgg
     1561 ttaactcgac atcttggtta ccgtgaagtt accatcacgg aaaaaggtta tgctgctttt
     1621 aagacccact ttcacattta agttgttttt ctaatccgca tatgatcaat tcaaggccga
     1681 ataagaaggc tggctctgca ccttggtgat caaataattc gatagcttgt cgtaataatg
     1741 gcggcatact atcagtagta ggtgtttccc tttcttcttt agcgacttga tgctcttgat
     1801 cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat aatgcattct
     1861 ctagtgaaaa accttgttgg cataaaaagg ctaattgatt ttcgagagtt tcatactgtt
     1921 tttctgtagg ccgtgtacct aaatgtactt ttgctccatc gcgatgactt agtaaagcac
     1981 atctaaaact tttagcgtta ttacgtaaaa aatcttgcca gctttcccct tctaaagggc
     2041 aaaagtgagt atggtgccta tctaacatct caatggctaa ggcgtcgagc aaagcccgct
     2101 tattttttac atgccaatac aatgtaggct gctctacacc tagcttctgg gcgagtttac
     2161 gggttgttaa accttcgatt ccgacctcat taagcagctc taatgcgctg ttaatcactt
     2221 tacttttatc taatctagac atcattaatt cctaattttt gttgacactc tatcattgat
     2281 agagttattt taccactccc tatcagtgat agagaaaagt gaaatgaata gttcgacaaa
     2341 gatcgcattg gtaattacgt tactcgatgc catggggatt ggccttatca tgccagtctt
     2401 gccaacgtta ttacgtgaat ttattgcttc ggaagatatc gctaaccact ttggcgtatt
     2461 gcttgcactt tatgcgttaa tgcaggttat ctttgctcct tggcttggaa aaatgtctga
     2521 ccgatttggt cggcgcccag tgctgttgtt gtcattaata ggcgcatcgc tggattactt
     2581 attgctggct ttttcaagtg cgctttggat gctgtattta ggccgtttgc tttcagggat
     2641 cacaggagct actggggctg tcgcggcatc ggtcattgcc gataccacct cagcttctca
     2701 acgcgtgaag tggttcggtt ggttaggggc aagttttggg cttggtttaa tagcggggcc
     2761 tattattggt ggttttgcag gagagatttc accgcatagt ccctttttta tcgctgcgtt
     2821 gctaaatatt gtcgctttcc ttgtggttat gttttggttc cgtgaaacca aaaatacacg
     2881 tgataataca gataccgaag taggggttga gacgcaatcg aattcggtat acatcacttt
     2941 atttaaaacg atgcccattt tgttgattat ttatttttca gcgcaattga taggccaaat
     3001 tcccgcaacg gtgtgggtgc tatttaccga aaatcgtttt ggatggaata gcatgatggt
     3061 tggcttttca ttagcgggtc ttggtctttt acactcagta ttccaagcct ttgtggcagg
     3121 aagaatagcc actaaatggg gcgaaaaaac ggcagtactg ctcggattta ttgcagatag
     3181 tagtgcattt gcctttttag cgtttatatc tgaaggttgg ttagttttcc ctgttttaat
     3241 tttattggct ggtggtggga tcgctttacc tgcattacag ggagtgatgt ctatccaaac
     3301 aaagagtcat cagcaaggtg ctttacaggg attattggtg agccttacca atgcaaccgg
     3361 tgttattggc ccattactgt ttgctgttat ttataatcat tcactaccaa tttgggatgg
     3421 ctggatttgg attattggtt tagcgtttta ctgtattatt atcctgctat cgatgacctt
     3481 catgttaacc cctcaagctc aggggagtaa acaggagaca agtgcttagt tatttcgtca
     3541 ccaaatgatg ttattccgcg aaatataatg accctcttga taacccaaga gggcattttt
     3601 tacgataaag aagatttagc ttcaaataaa acctatctat tttatttatc tttcaagctc
     3661 aataaaaagc cgcggtaaat agcaataaat tggccttttt tatcggcaag ctcttttagg
     3721 tttttcgcat gtattgcgat atgcataaac cagccattga gtaagttttt aagcacatca
     3781 tcatcataag ctttaagttg gttctcttgg atcaatttgc tgacaatggc gtttacctta
     3841 ccagtaatgt attcaaggct aattttttca agttcattcc aaccaatgat aggcatcact
     3901 tcttggatag ggataaggtt tttattatta tcaataatat aatcaagata atgttcaaat
     3961 atactttcta aggcagacca accatttgtt aaatcagttt ttgttgtgat gtaggcatca
     4021 atcataatta attgctgctt ataacaggca ctgagtaatt gttttttatt tttaaagtga
     4081 tgataaaagg cacctttggt caccaacgct tttcccgaga tctcatctat tgaaacagct
     4141 tgatagcctt tttcaacaaa caatattcgt gctgagttaa ccagtgattg ataggtactc
     4201 ttaaaatttt cttgttgatg atttttattt tccatgatag atttaaaata acataccgtc
     4261 agtatgttta tggtatcatg atgatgtggt cgtgacaatc ttaagaacat ttaggttatt
     4321 ttatgtatat tgaacagcat tctcgctatc aaaataaagc taataacatc caattaagat
     4381 atgatgataa gcagtttcat acaacggtta tcaaagatgt tctattatgg attgaacata
     4441 atttagatca gtctttactg cttgatgatg tggcgaataa agcgggttat accaagtggt
     4501 attttcagcg gctgttcaaa aaagtaacag gggtcacact ggctagctat attcgtgctc
     4561 gtcgtttgac gaaagcggct gttgagttga agttgacgaa aaaaactatc cttgagatcg
     4621 cattacaata tcaatttgat tcccaacaat cttttacacg tcgatttaag tacattttta
     4681 aggttacacc aagttattat cggcgtaata aattatggga attggaggca atgcactgag
     4741 agatcccctc ataatttccc caaagcgtaa ccatgtgtga ataaattttg agctagtagg
     4801 gttgcagcca cgagtaagtc ttcccttgtt attgtgtagc cagaatgccg caaaacttcc
     4861 atgcctaagc gaactgttga gagtacgttt cgatttctga ctgtgttagc ctggaagtgc
     4921 ttgtcccaac cttgtttctg agcatgaacg cccgcaagcc aacatgttag ttgaagcatc
     4981 agggcgatta gcagcatgat atcaaaacgc tctgagctgc tcgttcggct atggcgtagg
     5041 cctagtccgt aggcaggact tttcaagtct cggaaggttt cttcaatctg cattcgcttc
     5101 gaatagatat taacaagttg tttgggtgtt cgaatttcaa caggtaagtt agttgctaga
     5161 acccatggct cctttgccga cgctgagtag attttaggtg acgggtggtg acaatgagtc
     5221 cgtgtcgagc gctgattttt tcggccttta gagcgagatt tatacaatag aatttggcat
     5281 gagattggat tgcttttagt cagcctctta tagcctaaag tctttgagtg actagatgac
     5341 atatcatgta agttgctgat aggtttccag ttttccgctc ctaggtctgc atattgtact
     5401 tttcctctta ctcgacttaa ccagtaccaa cccagcttct caacggattt ataccatggc
     5461 actttaaagc cagcatcact gacaatgagc ggtgtggtgt tactcggtag aatgctcgca
     5521 aggtcggcta gaaattggtc atgagctttc tttgaacatt gctctgaaag cgggaacgct
     5581 ttctcataaa gagtaacaga acgaccgtgt agtgcgactg aagctcgcaa taccataagt
     5641 cgtttttgct cacgaatatc agaccagtca acaagtacaa tgggcatcgt attgcccgaa
     5701 cagataaagc tagcatgcca acggtataca gcgagtcgct ctttgtggag gtgacgatta
     5761 cctaacaatc ggtcgattcg tttgatgtta tgttttgttc tcgctttggt tggcaggtta
     5821 cggccaagtt cggtaagagt gagagtttta cagtcaagta atgcgtggca agccaacgtt
     5881 aagctgttga gtcgttttaa gtgtaatt
//
LOCUS       DAJTNN010000213         5835 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTNN010000213 DAJTNN010000000
VERSION     DAJTNN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.216"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(87..2189)
                     /locus_tag="OO150_003765"
     CDS             complement(87..2189)
                     /locus_tag="OO150_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5517744.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPSDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAVGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2431..3492
                     /locus_tag="OO150_003766"
     CDS             2431..3492
                     /locus_tag="OO150_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR5517745.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3605..5104)
                     /gene="ansP"
                     /locus_tag="OO150_003767"
     CDS             complement(3605..5104)
                     /gene="ansP"
                     /locus_tag="OO150_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR5517746.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5371..>5835
                     /locus_tag="OO150_003768"
     CDS             5371..>5835
                     /locus_tag="OO150_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5517747.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLTLENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQL"
BASE COUNT         1550 a         1502 c         1390 g         1393 t
ORIGIN      
        1 gaaattatgc aacaaaatat tgctatttta taccagcaat acaatcgtta aagtaaatgc
       61 ccggcaaata ccgggcatca aggtaattac tcaaatctcc acgcaatatt catgccgaca
      121 ccataatttc gtccgggcga aggttcgtaa tatcgcccgt ttgactcatt gacaatgaca
      181 gaaccaacgt attctttatc gaataaatta tcgacacgac caaataaatc cacagttaaa
      241 ttgtggtaat tgtatttata cccggtgaat aagccgacga gagtataaga cggcgctttt
      301 gccgtatttt catcatctgc cataatatcg cccatataac gcgcttctgt gcctgcatac
      361 caaccatctt ccggtacata acctatcgac gcaaagccca tattacgggc gatccctggc
      421 atccgattac cgttacaatc ctgttcattg caaacattgc tgcgataggt cgcatcaagc
      481 caggtccagg acgcgtttac gcgaaaatct cctgcgaaac gttgatccca tgccagttca
      541 gcgccttgac gacgggtctt tccggcattt ttgtaagtcg tacgcccacc gctactgcta
      601 tcgacaacaa tttcatcatc agtgtcggtc tgaaacaatg cgagactaag cagcccatca
      661 ccaatacgcg ttttactgcc gatctcaatt gtatcgttgg tggatggttt taaaccaaag
      721 ttcataccgc tttgcccatc agcacgataa gacagctcat taatcgtcgg cgtttcaaaa
      781 cctcgcccga ctgccagata gatattccag gcatcggtca ttgcatattt taacgaaccg
      841 gcaggtagcc atttatgata actggcatca ccgctgtcat cgccgttacc cggagtaacg
      901 taatggtcgt tggaatcaaa ccacacggag ctgtagcgca cgccagcatc cagcgacagt
      961 ttttcgctca gctgccactg cgtctgtaaa tagggatcga tgttccacat cagattgcgt
     1021 tcgtcgcgac gcaactcacc tttttgcccg tactccggca tgccgctatt caggcggaag
     1081 ttattgtagc ccttgcggtt ttcactcatg ttttcgtagt tcaggccggt agtgaacgtg
     1141 accggaacgc ccagttcacc acggtgtgtc cagcggctgt ctatcccctg gtaatggcgt
     1201 tgcagggtaa tcacgccgcc cgcatgtgac gggttaagtt gtggtgccat gggtattgac
     1261 tggtactggg tcgtttctcg ctctccggca tacatcatca cactcatatc atcttgcgcg
     1321 ctcaggctac gctcatagcg caaccctgcc tgagtttgct tgatggtttt tcgcgtgtcg
     1381 tactgttctg cacgaggcgc ttgttgtgga ttagccttcc attctgcttt ggttagccca
     1441 cctgggtcat ctgctttgat atccacacta ttgaaaatca gacttaattt gctggcttca
     1501 tcaatgcgta cgcccagttt ggcattggct aaatttttct gtgcgccact atggtcacga
     1561 tagccgtggg tcgtaaaacg cgtggttgag acggtgtaat cgacatcgct aggctgtgtg
     1621 ccgtctcccg ttgcgcccgt tgctttcagc ccatagcgcc agctgccaaa actgccgtag
     1681 taactactgg cttcaatggt tggtggctgt tgtccggtct gggtggtgac attcattacc
     1741 ccaccagacg cgttgccata cagggcagag aaggggccac gcagcacttc cacattttgc
     1801 acactgctta aatcgatgtt ggatgtttgc ccttgcccgt cgggcatggt ggcgggaata
     1861 ccgtccacat acaggcgaat accgcgaata ccgtaagtgg agcgggagcc aaatccgcga
     1921 atcgacagct gtaaatcttg cgcatagttc tgccggtttt gtacctgcaa accaggcacg
     1981 ccggtcagtg attcggacaa gttaatgcgc ggtgttgcca ggcgcatctc ctcgccatcc
     2041 accacgctta ctgctgctgg ggtatccagt tctgaaacca cctgcggtgc ggcactgaca
     2101 atcatagtct gttcatcagc ggcaaaaaca acgggggaaa ggacaagcag tgcgggcaaa
     2161 acggtctgtc ggacggaaaa aatcttcatg aaaaaagcca ggttaagaat gggaaaacgc
     2221 tgtcatggta ataaaattgt aaacttatgc aaaacgaaac ggcacgatac gttaattaat
     2281 tgagaaaatt gtagtcgtaa cggcaagaaa tgctccacat ttgagaaaat aatgattacc
     2341 attcccattt ataacaagag cgtgacgatg attacgctta gcgaagcatt gtgaagcagc
     2401 aaaaatattg gttcatcaaa gggagtcgtc atgcatttac gtcatctgtt ttcatcgcgc
     2461 ctgcgtggtt cattactgtt aggttcattg cttgttgctt catcattcag tacgcaggcc
     2521 gcagaagaaa tgctgcgtaa agcggtaggt aaaggtgcct acgaaatggc ttatagccag
     2581 caagaaaacg cgctgtggct cgccacttcg caaagccgca aactggataa aggtggcgtg
     2641 gtttatcgtc ttgatccggt cactctggaa gtgacgcagg cgatccataa cgatctcaag
     2701 ccgtttggtg ccaccatcaa taacacgact cagacgttgt ggtttggtaa caccgtaaac
     2761 agcgcggtca cggcgataga tgccaaaacg ggcgaagtga aaggccgtct ggtgctggat
     2821 gatcgtaagc gcacggaaga ggtgcgcccg ctgcaaccgc gtgagctggt agctgatgat
     2881 gccacgaaca ccgtttacat cagtggtatt ggtaaagaga gcgtgatttg ggtcgttgat
     2941 ggcgagaata tcaaactgaa aaccgccatc cagaacaccg gtaaaatgag taccggtctg
     3001 gcgcttgata gcaaaggcaa acgtctttac accactaacg ctgacggcga attgattacc
     3061 atcgacaccg ccgacaataa aatcctcagc cgtaaaaagc tgctggatga cggcaaagag
     3121 cacttcttta tcaacatcag ccttgatacc gccaggcagc gtgcatttat caccgattct
     3181 aaagcggcag aagtgttagt ggtcgatacc cgtaatggca atattctggc gaaggttgcg
     3241 gcaccggaat cactggctgt gctgtttaac cccgcgcgta atgaagccta cgtaacgcat
     3301 cgtcaggcag gtaaagtcag tgtgattgac gcgaaaagct ataaagtggt gaaaacgttc
     3361 gatacgccga ctcatccaaa cagcctggcg ctgtctgccg atggcaaaac gctgtatgtc
     3421 agtgtgaaac aaaaatccac taaacagcag gaagctaccc agccagacga tgtgattcgt
     3481 attgcgctgt aatagatatg gcgagggtga aaacggtgag acttgcctgc cttttatcac
     3541 ggcgtatccg gcactttcgt cagtaatctg aatttgcccg gcacgtagcc gggcaatttt
     3601 gcaattaact cgccgtttca ggcttaaaca caatttcctg tttttcttca tcttcctcga
     3661 cgactggcgc agtgctgtga atttcagcaa cgcgtttgcg cacaccaaac cagcctataa
     3721 ccagcagaat accgataatc ggcagcgccg cgatagtata agtcccgttc gggtaatcga
     3781 acgccatcag cacaaggaca ctcagtaaaa acagtaatgt cagccaggaa gtgaagggcg
     3841 cgccaggcag tttaaaactg acatccgctg ctttgccttc tttaatcgct ttacgcaggc
     3901 gcatctggca cacgatgata aacgcccatg aagcgatgat tcccagcgac gcgaagttca
     3961 acacaatctc aaatacgcgc gacggcacca gatagttgag gaatacgccg acgacataca
     4021 caaccagtgt cgctagaatc ccggcatacg gcacatgctg acgactcatt ttcgccataa
     4081 aactcggtgc ggaaccgccc atcgccattg agcgcagaat acgtccggtg cagtacagac
     4141 ctgagttcag gctggagagg gcagcggtca gcaccacaat gttcataatg ctgccgatat
     4201 atggcacacc cagtttagag aaaaacgtca cgaacggact ttgccccgcc tgatacgcgc
     4261 tccacggcaa taacataacc agcaacacca cggagccgac gtaaaacagg ccaatacgcc
     4321 aaatcacact gttaatggct ttaggcacca tggtctgcgg atctttacat tctccggcag
     4381 ctgtacccac catttcaatg gaggcaaaag caaacactac gccctgaatc aacaccagcg
     4441 caggcagcaa accgtggggg aagaagccgc cattatcggt gattaaatga aagccagtgg
     4501 tgttgccatc cagcggctga ccactaccga ggaacactgt acccacgacc aaaaaggtca
     4561 caatggcgag cactttaata agcgcaaacc agaactccat ctccgcaaac catttcacac
     4621 cgatcatatt catggtgcca acgatggtaa gtgcagcgag cgcaaagacc cactgcggca
     4681 cgccgccaaa cgcaccccag taatgcatat acagagcgac ggcggtaata tcaacaatcc
     4741 ccgtcatcgc ccagttgatg aagtacatcc agccagcaac ataagcggct ttctcaccca
     4801 aaaactcacg ggcataagag acaaaactgc cactggaagg gcggtgtagc accagctcac
     4861 ccaatgcacg cagaataaaa aacgaaaaca agccacaaat taaataaacc agtgccagtg
     4921 ctggacccgc catttgcagt cgggctcctg cacctaaaaa caagccggtg ccaatcgcgc
     4981 cgccaatggc gatcatctgt acctggcgat tgcccatcgc tttgtgatac ccctcttcgt
     5041 gggcattaag ccagcggcgt ttcgcggcgt gttgatctga agtgtcggtg tcgtgtttac
     5101 tcattgctct ccctgattgc tttaatgaaa aagtcatata agttgccatg aacaatgttt
     5161 attcatcctg gcgatgatag ttatcaccat tattctttat gggtataaaa ctatcggcgc
     5221 agcatcctac ccgttatcga tatacgatgc aaaacatccc cttacaatcc tgaaggggat
     5281 tcatacaact gacgaaaaaa tgacaaatac ttttgctggt taacctgtgt actgtcctac
     5341 acttaatctt taaaagattg tgaggggcat atgattaagg tgtatggcgt acccggatgg
     5401 ggctctacaa tcagtgagct gatgctaaca ctggctgata ttccttatca gttcgttgat
     5461 gtcagtggtt ttgaccacga gggaacttca cgcgagttat tgaaaaccct gaacccgttg
     5521 tgtcagattc cgacactgac gctggaaaat gacgagatta tgacggagac agcggcgatt
     5581 gcattgatgg ttctcgatcg tcgcccggac cttgcgccac cggttgggcg cgccgaacgt
     5641 cagcagtttc aacggctatt ggtctggctg gttgctaatg tctatccaac attcactttc
     5701 gccgattacc ctgagcgttg ggctcctgac gcacctgaac agttaaagaa gaatgttatt
     5761 gaatatcgta aatcgcttta tatctggctg aattcacaat tgaccgctga accttatgcg
     5821 tttggtgagc aacta
//
LOCUS       DAJTNN010000214         5805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTNN010000214 DAJTNN010000000
VERSION     DAJTNN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.217"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..846
                     /gene="pdeC"
                     /locus_tag="OO150_003769"
     CDS             <1..846
                     /gene="pdeC"
                     /locus_tag="OO150_003769"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5517748.1"
                     /translation="PAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQPI
                     IDIKTEKCIGAEALLRWPSEQEQVMNPAEFIPLAEKEGMIEQVTNYVIDNVFRDLGDY
                     LATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMTP
                     IILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHI
                     IELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPARE
                     LTRGQ"
     gene            complement(849..1172)
                     /gene="soxS"
                     /locus_tag="OO150_003770"
     CDS             complement(849..1172)
                     /gene="soxS"
                     /locus_tag="OO150_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5517749.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1258..1722
                     /gene="soxR"
                     /locus_tag="OO150_003771"
     CDS             1258..1722
                     /gene="soxR"
                     /locus_tag="OO150_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5517750.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYRRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2268..3617
                     /gene="ghxP"
                     /locus_tag="OO150_003772"
                     /gene_synonym="yjcD"
     CDS             2268..3617
                     /gene="ghxP"
                     /locus_tag="OO150_003772"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5517751.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3769..5418
                     /locus_tag="OO150_003773"
     CDS             3769..5418
                     /locus_tag="OO150_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5517752.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5572..>5805)
                     /locus_tag="OO150_003774"
     CDS             complement(5572..>5805)
                     /locus_tag="OO150_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5517753.1"
                     /translation="MFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1258 a         1375 c         1599 g         1573 t
ORIGIN      
        1 cccgccggta ttttggggag tcttgttctg ctattactct ggctacgtat tcgacaaaac
       61 tatttgtctc ccaaacgcaa attgcaacgc gctctagaaa aacatcaact ttgtctttat
      121 taccagccaa taatcgatat caaaacagaa aaatgtatcg gcgctgaagc gttgttacgt
      181 tggcctagtg agcaggagca agtaatgaat ccggcagagt ttattccgct ggcagaaaag
      241 gaggggatga tagaacaggt aactaattat gttattgata atgtcttccg cgatctgggc
      301 gattacctgg caacacatgc agatcgctat gtttctatta acctgtcggc ctccgatttt
      361 catacgtcac ggttgatagc gcgaatcaat cagaaaacag agcaatacgc ggtgcgtccg
      421 cagcaaatta aatttgaagt gactgagcat gcatttcttg atgtcgaaaa aatgacgccg
      481 attattctgg ctttccgcca ggctggttac gaagtggcaa ttgatgattt tggtattggc
      541 tactctaact tgcataacct taaatcattg aatgtcgata ttttgaaaat cgataaatcg
      601 tttgttgaaa cgctgaccac ccacaaaacc agtcatttga ttgcggaaca catcatcgag
      661 ctggcgcaca gcctggggtt aaaaacgatc gctgaaggcg tcgaaactga ggagcaggtt
      721 aactggctgc gcaaacgcgg cgtgcgctat tgccagggat ggttttttgc gaaggcgatg
      781 ccgccgcagg tgtttatgca atggatggag caattacccg cgcgggagtt aacgcgcggg
      841 caataaaact acaggcggtg gcgataatcg ctgggagtgc gatcaaactg ccgacggaaa
      901 acgcgggaga aggtctgctg cgagacataa cccaggtcca ttgcgatatc aaaaatcgga
      961 cgctcggtgg tgcgcaactc aacggcggcc agtaacaggc gacgttggcg aatgtaatcg
     1021 ccaagcgtct gatgcgtcac cgtgcggaac attcgttgca agtaccactt tgaatagcct
     1081 gatttttttg cgactacatc aatgttaagc ggctggtcaa tatgctcgtc aatccatgcg
     1141 ataagatcct gaataatttt ctgatgggac ataaatctgc ctcttttcag tgttcagttc
     1201 gttaattcat ctgttgggga gtataattcc tcaagttaac ttgaggtaaa gcgatttatg
     1261 gaaaagaaat taccccgcat taaagcgctg ctaacccccg gcgaagtggc gaaacgcagc
     1321 ggtgtggcgg tatcggcgct gcatttctat gaaagtaaag ggttgattac cagtatccgt
     1381 aacagcggca atcagcggcg atatagacgt gatgtgttgc gatatgttgc aattatcaaa
     1441 attgctcagc gtattggcat tccgctggcg accattggtg aagcgtttgg cgtgttgcca
     1501 gaagggcata cgttaagtgc gaaagagtgg aaacagcttt cgtcccaatg gcgagaagag
     1561 ttggatcggc gcattcatac cttagtggcg ctgcgtgacg aactggacgg atgtattggt
     1621 tgtggctgcc tttcgcgcag tgattgcccg ttgcgtaacc cgggcgaccg cttaggagaa
     1681 gaaggtaccg gcgcacgctt gctggaagat gaacaaaact aaagcgccac aagggcgctt
     1741 tagtttgttt tccggtcttt gtctttctct ctatcccgct ggtacacagg agggtttccc
     1801 ccgacgtcaa cacacctcat tcgagcacgt ggtggaggtt ccggttggtg ttgatgcttt
     1861 aattgtatgt caccgacgtt tcttcgccag tgtaaaagta tactttttaa ccgcaatatt
     1921 tttgtcatct cagacgattt tttatcgcaa tcctgaacgg tatacggctc gataacgctg
     1981 caatcttgcg caccgacgat aacgtttgcg catcaattgc ctgttttttc atcgtcaaga
     2041 caataaaaga gaaaaaagca gcaaacttcg gttgaaaaag ccgctatgat cgccggataa
     2101 tcgtttgctt tttttaccac ccgttttgta tgcgcggagc taaacgtttg cttttttgcg
     2161 acgcagtaaa ttgtcgcaaa cctggagcag gaagataacg tttcgctggc aggggattgt
     2221 ccgccacgca tcttgacgaa aattaaactc tcaggggatg ttttcttatg tctacgccat
     2281 cagcgcgtac cggcggttca ctcgacgcct ggtttaaaat ttcacaacgt ggaagcactg
     2341 tccgtcagga agtggttgcc gggttaacaa cgtttctggc gatggtctac tcggtcatcg
     2401 tcgttccagg tatgttgggt aaagcaggct tcccgcctgc ggcagttttc gttgcaacct
     2461 gtctggttgc cggactcggt tctatcgtga tgggtctgtg ggctaatctg ccgttggcga
     2521 ttggttgcgc catctccctg acggcgttta ccgcattcag cctggtgctg gggcaacata
     2581 ttagcgtacc tgtcgcgctg ggtgccgtgt tcctgatggg tgtgctgttt acggtaattt
     2641 ctgccacggg tatccgtagc tggattttgc gcaacttgcc gcacggtgtg gcgcacggca
     2701 cggggatcgg tatcggtctg ttcctgctgc tcattgccgc taatggtgtt ggtctggtga
     2761 ttaaaaaccc gcttgatggt ctgcccgttg cgctgggtga tttcgcgacc ttcccggtga
     2821 ttatgtcact ggtaggtctg gcggtgatca tcggcctgga aaaactgaaa gtccctggtg
     2881 gcattctgct gaccattatc ggtatctcaa ttgtcggttt gatcttcgat cctaacgtcc
     2941 atttctccgg cgttttcggc atgccttcat tgagcgatga aaacggcaat tcactgattg
     3001 gcagcctgga cattatgggc gcgctgaatc ctgtagtcct gccaagcgtt ctggcgctgg
     3061 tgatgacggc agtatttgat gccaccggaa ctatccgtgc cgtcgccggc caggcgaacc
     3121 tgctggataa agatgggcag atcatcgacg gtgggaaagc actgaccact gactccatga
     3181 gcagcgtttt ctctggcctg gtgggtgctg ctccggcagc ggtatacatc gagtctgcgg
     3241 cgggtacggc ggcgggcggt aaaaccggtt tgacggctat caccgttggc gtgctgttcc
     3301 tgctgattct gttcctctct ccgctctctt acctcgttcc ggggtatgca acggctccgg
     3361 cgctgatgta cgttggcctg ctgatgctga gcaacgtggc gaaaatcgac tttgctgatt
     3421 ttgttgatgc gatggcgggt ctggttacgg cggtattcat cgtgctgacc tgtaacatcg
     3481 taacaggcat catgatcggc ttcgcgactc tggtgattgg tcgtctggtt tccggcgaat
     3541 ggcgcaagtt gaacatcggt acggtcgtta tcgccgtggc gctggtgacc ttctatgcgg
     3601 gtggctgggc tatctaatct ttcatcggat tctgaaaacg ggtggcaatg gctgcccgtt
     3661 tttattttct ccgcacattg tgtgagcttt tgcgatattc tgaaaaaatg agaattcagg
     3721 cataacgcct ttctcagatc ataaaaaaac atcgcaaaca gggaacgcat ggaaatcttc
     3781 ttcaccatac tgataatgac cctcgtggtc tcgctgtccg gggtggtcac tcgtgtcatg
     3841 ccctttcaga tcccgcttcc gcttatgcaa atcgccatcg gtgcgctact ggcgtggccg
     3901 acgtttggtt tgcatgtgga gtttgatcct gaactctttt tagtcttgtt tatcccgccg
     3961 ttgctgttcg ctgatggctg gaaaacgccg acccgtgaat ttctcgaaca tggtcgagag
     4021 attttcggcc tcgcgctggc gctggtggtg gtcaccgtgg tcggcattgg cttccttatt
     4081 tactgggtgg tgccgggcat tccgctgatc cccgcctttg cgctggcggc ggtactttct
     4141 ccgaccgatg ctgtggcgct ctccggaatt gttggcgaag ggcgcatccc gaaaaaaatc
     4201 atgggcattt tgcagggcga agcgttgatg aatgacgcct ccggcctggt gtcgttgaag
     4261 tttgccgtgg cagtggcgat ggggacgatg atcttcaccg tcggcggtgc gacggtcgaa
     4321 tttatgaaag tagccattgg tggtattctt gccggttttg tggtgagctg gctgtatggt
     4381 cgctcgctgc gattcctcag ccgctggggc ggtgatgaac ccgcgacgca gatcgttctg
     4441 ctgttcttgc tgccattcgc ttcttatctg attgccgaac atattggcgt ttccggcatc
     4501 ctcgctgcgg ttgccgccgg gatgaccatc acccgctccg gtgtgatgcg ccgtgcgccg
     4561 ctggcaatgc gcctgcgcgc aaacagcacc tgggcgatgc tggaatttgt ctttaacggc
     4621 atggtgttcc tgctgttagg tctgcagctg ccgggtattc tggagacgtc gctgatggcg
     4681 gcagaaatcg accctaacgt cgaaatctgg atgctgttta ccgatattat tctgatatat
     4741 gcggcgctga tgctggtccg tttcggctgg ctgtggacga tgaaaaagtt cagcaaccgc
     4801 ttcctgaaga agaagccgat ggagtttggt tcgtggacca cacgagaaat cctgatcgcg
     4861 tctttcgccg gggtgcgtgg ggcgatcact ctggccggtg tgctctctat cccgctgctc
     4921 ttgccggatg gtaacgtctt cccggcgcgc tatgagctgg tgttcctggc ggctggcgtc
     4981 attctcttct cgctgtttgt cggtgtggtg atgttgccta ttctgctaca acacattgaa
     5041 gtcgcggatc attcgcaaca attgaaagag gaacgtattg cgcgagcggc aacggcagaa
     5101 gtggcgattg tggcgatcca gaaaatggag gagcgtctgg cggcggatac cgaagagaat
     5161 atcgataacc agctgcttac ggaggtcagt tctcgcgtca ttggtaacct gcgtcgtcgc
     5221 gccgatggac gtaacgacgt tgaaagttcc gtgcaggaag agaaccttga gcgtcgcttc
     5281 cgtctggcgg cattgcgttc tgaacgtgct gaactgtacc acctgcgcgc cacgcgggag
     5341 atcagcaacg aaacgctgca aaaattactg cacgatctcg atttgcttga agcgttgcta
     5401 attgaggaaa atcagtaagc cgaaagcgac gtaaccaggc ccgaggtaat ctgaaaaagg
     5461 tgcaatcttt tgcaccttta acaggttagc tcgggctaac attcatgatt ctaaaacaaa
     5521 taagttatct ctcttgtaaa gtcaattcat tggctgttct tcgacttaac ctcaatctgc
     5581 gtgttgcata atatctgcag taatcttcat agcgactgca ttaatattat cctcagtttg
     5641 tgacatgcca gtatgagagg tacaatcttc ttcaaagaat ttctgtagtg ttaccagggt
     5701 atcactatca atggggatat tttcctctcc acggagtatg gaaatgccca ttccctggac
     5761 gctagcctca tcaaaattaa cccgatagag acgggtatta aacat
//
LOCUS       DAJTNN010000215         5755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTNN010000215 DAJTNN010000000
VERSION     DAJTNN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.218"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..393)
                     /locus_tag="OO150_003775"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO150_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO150_003776"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO150_003776"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5517754.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISHSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO150_003777"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO150_003777"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5517755.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQLNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1779 a         1301 c          952 g         1723 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tgctcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaacta
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgac cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagtagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac cgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgta atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagct tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tatagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg tagattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggaa tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctaaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcatcaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac gttttactga aaataataac tttcggaata tattctccac
     4561 acgaaactct agccgaaaaa tatagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt tcccgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaat taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc cgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccgg
//
LOCUS       DAJTNN010000216         5680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTNN010000216 DAJTNN010000000
VERSION     DAJTNN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.219"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..182)
                     /locus_tag="OO150_003778"
     CDS             complement(<1..182)
                     /locus_tag="OO150_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR5517756.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLG"
     gene            708..1403
                     /gene="minC"
                     /locus_tag="OO150_003779"
     CDS             708..1403
                     /gene="minC"
                     /locus_tag="OO150_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5517757.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1427..2239
                     /gene="minD"
                     /locus_tag="OO150_003780"
     CDS             1427..2239
                     /gene="minD"
                     /locus_tag="OO150_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5517758.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2243..2509
                     /gene="minE"
                     /locus_tag="OO150_003781"
     CDS             2243..2509
                     /gene="minE"
                     /locus_tag="OO150_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5517759.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2988..3107)
                     /locus_tag="OO150_003782"
     CDS             complement(2988..3107)
                     /locus_tag="OO150_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5517760.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3068..3253)
                     /locus_tag="OO150_003783"
     CDS             complement(3068..3253)
                     /locus_tag="OO150_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517761.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3354..3527
                     /locus_tag="OO150_003784"
     CDS             3354..3527
                     /locus_tag="OO150_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517762.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVRATYLIPDI"
     gene            3529..3873
                     /locus_tag="OO150_003785"
     CDS             3529..3873
                     /locus_tag="OO150_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5517763.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3877..4212
                     /locus_tag="OO150_003786"
     CDS             3877..4212
                     /locus_tag="OO150_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5517764.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4269..>5680)
                     /locus_tag="OO150_003787"
     CDS             complement(4269..>5680)
                     /locus_tag="OO150_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5517765.1"
                     /translation="DSIINLSYDDGQTFTQGKTLTVKGNYVGNNGQLNIRTVLGDDKS
                     ATDRLIVEGNTSGSTTVYVKNAGGSGAATLNGIELITVNGDESPAEAFRQGDARIAAG
                     AFEYQLKQQGKNWYLTSYQSVNEEDNSSEGNSEPTETPTPVLRPEAGSYVANLAAANT
                     LFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSHRYVSQLG
                     ADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYCAGLYATWFADDI
                     SKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKY
                     TWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGD
                     DFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGNLNPAASVWGNVGVQ
                     LGDNGYNDTAVMVGLKYKF"
BASE COUNT         1549 a         1322 c         1319 g         1490 t
ORIGIN      
        1 tcgccgagat atttccttgt taactctggg gaaataccat ctgagttcac acaaatatac
       61 gggtcgcaaa caacgccgca cgccggagat tgaagtccgc cagcgtcatg tccagacgcc
      121 attgatgaga acatcatccc cacacctgac aatccgatat aaaaaatacg catcatattc
      181 atagcacatc ctgataatcc aaccgtacaa cacttatgct catttcatca tcaatccgta
      241 atcaaaaccg ctgacctgga cgagttacga tgacctgaca gaagtatcag acgtcttctg
      301 cggtttctat gattatgata gagcaattct atggcggata ttaagataac gggtatctgt
      361 tctttaatga tgctgcaaaa aatggaaagt taagaaagcc tggcgtggtg aaaaaataca
      421 cttgcctcaa tactggatat ttaaacaggt ctggagatgc tgtcacgatt agggtgcgga
      481 aaaaggaagc gtaaataaga acaatcaaaa gtacgacggc aatgggttga ttgacaaggg
      541 tattttttaa gctatgaatc agcgccattt atcacagaat agacttttac tctgaataaa
      601 tgggagggtg acttgcctca atataatcca gactataaca tgccttatag tcttcggaac
      661 atcatcgcgc gctggcgatg attaatagct aattgagtaa ggccaggatg tcaaacacgc
      721 caatcgagct taaaggcagt agcttcactt tatctgtggt tcatctgcat gaggcagaac
      781 ctaaggttat ccatcaggcg ctggaagaca aaatcgctca ggcccccgca tttttaaaac
      841 atgcccccgt tgtactcaac gtcagtgcac tggaagaccc ggtaaactgg tcagcgatgc
      901 ataaggcggt gtcggcaacc ggtttgcggg ttattggcgt aagtggctgc aaagatgcgc
      961 aacttaaagc cgaaattgaa aagatggggc tgcctatcct gacggaagga aaggaaaaag
     1021 cgccgcgtcc agctcccgca ccacaggctc cagcgcaaaa tacaacgccg gtcacaaaaa
     1081 cgcgtttaat agataccccg gtgcgttccg gtcagcgtat ttatgctcca caatgtgatc
     1141 tgattgttac aagccacgtt agcgctgggg ccgaattgat tgccgatggg aacattcatg
     1201 tctatggcat gatgcgcggt cgtgcgcttg caggggcaag tggtgaccgg gaaacgcaaa
     1261 tattttgtac gaacctgatg gcggaactgg tgtccatcgc aggtgaatac tggctgagtg
     1321 atcaaatccc agcagaattt tatggcaaag cggcgcgact gcagttagtc gaaaacgctt
     1381 tgaccgttca accgttaaat tgatcccttt ttaacaagga atttctatgg cacgcattat
     1441 tgttgttact tcgggcaaag ggggtgttgg taagacaacc tccagcgcgg ccatcgccac
     1501 tggtttggcc cagaagggaa agaaaactgt cgtgatagat tttgatatcg gcctgcgtaa
     1561 tctcgacctg attatgggtt gtgaacgccg ggtcgtttac gatttcgtca acgtcattca
     1621 gggcgatgca acgctaaatc aggcgttaat taaagataag cgtactgaaa atctctatat
     1681 tcttccggca tcgcaaacac gcgataaaga tgccctcacc cgtgaagggg tcgccaaagt
     1741 tcttgatgat ctgaaagcga tggattttga atttatcgtt tgtgactccc cggcagggat
     1801 tgaaaccggt gcgttaatgg cactctattt tgcagacgaa gccattatta ccaccaaccc
     1861 ggaagtctcc tcagtacgcg attctgaccg tattttaggc attctggcgt cgaaatcacg
     1921 ccgcgcagaa aatggcgaag agcctattaa agagcacctg ctgttaacgc gctataaccc
     1981 aggccgcgta agcagaggtg acatgctgag catggaagat gtgctggaga tcctgcgcat
     2041 caaactcgtc ggcgtgatcc cagaggatca atcagtgttg cgcgcctcta accagggtga
     2101 accggtcatt ctcgacatta acgccgatgc gggtaaagcc tacgcagata ccgtagaacg
     2161 tctgttggga gaagaacgtc ctttccgctt cattgaagaa gagaagaaag gcttcctcaa
     2221 acgcttgttc ggaggataag ttatggcatt actcgatttc tttctctcgc ggaagaaaaa
     2281 cacagccaac attgcaaaag aacggctgca gattattgtt gctgaacgcc gtcgcagcga
     2341 tgcagaaccg cattatctgc cgcagttgcg taaagatatt cttgaggtca tttgtaaata
     2401 cgtacaaatt gatcctgaga tggtaaccgt acagcttgag caaaaagatg gcgatatttc
     2461 tattcttgag ctgaacgtga ccttaccgga agcagaagag ctgaaataag cccgctgtaa
     2521 aaacgcattt atcttcaagg cagagtcatt tctgccttga gtttttcatc cctctcatcc
     2581 acgttgtggt aaagcggcga gtattcttgc tgatactcct cattgctatt tccacctccc
     2641 cctttacacc ttaaggctgt tcccacgcac agaacaaaat gtttatgcct ggttgagtaa
     2701 ataacctcat tgttagtgtg gatacactgg gggatgataa ccccgcaatt tcattattca
     2761 ttgttctccc ataggatgag gtttcccgcc gtaactggcg ggcttttttt gccccagaat
     2821 tttccctttt agccccctgt aaacgaaaac tgcgccgaag cgcagtttaa tcgttgatgg
     2881 tcagaacgac cagttcacga tatatactgc gggtatcgac tgatttcgat tccatgccat
     2941 tggataaatg atagtagcgt ctaagtgacc taagctctgc tgcaacatca gcccgcaggc
     3001 accagaccag gggattcatc ctgaagagac agcgcaggtg tattgtgttc accactcacc
     3061 aaagacatca tgatgaaatg atgatatccc gcataagaaa gaggcatttt ttaaacgcag
     3121 tgcgctgaag tgtagttgga taaaaaatca ctcttttcag ataatactgt cgtacgctta
     3181 tctttcgaca atgaggccgt cggcaaaata aaatgactaa tgtaatcgtt gctgatgaat
     3241 atcttctgct cacataaaaa tcacacaata atattgataa atcagattat cttaccttta
     3301 caacattgcg ttaacagtca atgacacttc ttgaataaaa atattgccaa tcaatgagtt
     3361 attcctcatt taagattatt ctcattcacg taaaaaacat aattcctatc atcacagcca
     3421 cgctcatttt gaattatctg aataatagcg agcgttcttt agttaagcag atcttaattg
     3481 aagacgaaat tattgtgcgt gcaacttact taataccgga catctaagtt gaagaaaaaa
     3541 atacttgcct tcgggcttat ctcagcattg ttctgctcca cgccagcgat ggcggatatg
     3601 aatcgcacca cgaaaggtgc attgctaggc gcaggcgttg gtttacttac tggcaatggt
     3661 gttaatggcg tactgaaagg tgcagctgtt ggcgctggtg ttggtgcagt aacagaaaaa
     3721 ggccgcgacg gtaaaaatgc gcgtaaaggt gcaaaagtag gtgccgccgt tggtgctgtc
     3781 actggcgttc tgaccggcaa tggcctcgaa ggggcgatta aaggtgccgt gattggcggt
     3841 acgggtggtg caattctggg taaaatgaaa taaggaatga tgatgaaaaa atttgcttta
     3901 ctggcgggcc tgtttgtttt cgcaccaatg acctgggcgc aggactacaa tattaaaaac
     3961 ggtttaccgt cagaaaccta tattacttgc gcagaagcca acgaaatggc aaaaactgac
     4021 agcgcacagg ttgcagaaat tgttgcggta atgggtaatg ccagcgttgc cagccgtgat
     4081 ttaaaaattg agcaatctcc ggaactgagc gccaaagtgg tagaaaaact gaatcaggtt
     4141 tgcgctaaag acccgcaaat gctgttgatc acggcaattg atgacaccat gcgtgctatc
     4201 ggcaaaaaat aatatttcat atgtaccgcg tcgatgaata cgacgcggtt ttttagctaa
     4261 cggcgggatc agaacttata tttcaggccc accatcactg cggtgtcatt gtagccatta
     4321 tcacccagct gcacgcccac attcccccag acgctggccg ctggattcag gttgccattt
     4381 acgccaagtt taatttcccc caaattactc gccccatctt gttcgacttt cacaccgttc
     4441 attgagacag caaaatcttt actgttatgg atccagttca tttcaacaaa aggttcaaag
     4501 tcgtcaccgt gcggaccgct gtttttctcc tgagtacgaa taaaggtgcg gaaaccaagt
     4561 cgggtctgga tgttgttatt tgcgtcattc tcaatacgtg agccattggc ttcggtgtgc
     4621 gcattatgat cgacgcccat ccagatagcc tgtgcctgtg gctggaatat ccacgtgtat
     4681 ttcgccgctt ccagaccaaa ggatttattc agggcaaagc cgtaacccgc ttccagcgag
     4741 acggttgcgc ctttcgcact ataggattca taggctaatt catccccttt caccgagttt
     4801 ttaaaccagc tatattgcgc ccaggcgtca atgtatgcgc cttttttact gatgtcatcg
     4861 gcaaaccaag tggcatacag ccctgcgcaa tagcctctga cactcccttt cgaacgataa
     4921 tccgacacgc tggaatgagt tgagttgtag tcgcgggcat aaccagccat cactcccaaa
     4981 cgccaactgt cactgtcggt aaaaccaccg gttaacagat cggcccctaa ctgcgagacg
     5041 tagcgatgcg aggtcgttct caactgtccg ttgctgtcac gccaggcatt atgcccgcca
     5101 atttgacgta gccaaagtcg gcttgaacgc tcctgttcag ttacaggatc gatgtagcgc
     5161 atttcacccg cacggtcgtt cagacgcata acaaaaagag tgttagcggc tgccaggttg
     5221 gcgacgtaac ttccagcttc ggggcgcagg acgggggtag gcgtttctgt tggttctgaa
     5281 tttccctctg agctgttatc ctcctcattc actgactgat aacttgtcaa ataccagttt
     5341 ttgccttgtt gttttagttg atattcgaaa gctccagctg caatccttgc atcaccttgt
     5401 ctgaaggctt ctgctggaga ttcatcgcca ttcacagtta tgagttcgat cccgtttagc
     5461 gtggccgcgc cgcttcctcc agcatttttc acatagacgg tagttgaacc cgaagtatta
     5521 ccctcaacaa taagtctgtc cgtagccgat ttatcatcac ctaatacggt gcggatatta
     5581 agctgaccat tattaccgac ataattacct tttacggtta atgtttttcc ttgggtaaat
     5641 gtttgaccat catcatacga aagattgata atactgtctg
//
LOCUS       DAJTNN010000217         5625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTNN010000217 DAJTNN010000000
VERSION     DAJTNN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.222"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1161
                     /locus_tag="OO150_003788"
     CDS             <1..1161
                     /locus_tag="OO150_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5517766.1"
                     /translation="QMDGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPM
                     VAADVQNPHQPKTATGVIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKA
                     VDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIE
                     DNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDE
                     HYRYAAYGLMLQEQAPTLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQ
                     RMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSA
                     EGKARVQSLLAIKAIFGDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1197..1400)
                     /gene="ydfZ"
                     /locus_tag="OO150_003789"
     CDS             complement(1197..1400)
                     /gene="ydfZ"
                     /locus_tag="OO150_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5517767.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1578..2262)
                     /gene="ydfH"
                     /locus_tag="OO150_003790"
                     /pseudo
     CDS             complement(1578..2262)
                     /gene="ydfH"
                     /locus_tag="OO150_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
     gene            complement(2351..3097)
                     /gene="ydfG"
                     /locus_tag="OO150_003791"
     CDS             complement(2351..3097)
                     /gene="ydfG"
                     /locus_tag="OO150_003791"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5517768.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3234..5279
                     /gene="dcp"
                     /locus_tag="OO150_003792"
     CDS             3234..5279
                     /gene="dcp"
                     /locus_tag="OO150_003792"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5517769.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5324..>5625)
                     /locus_tag="OO150_003793"
     CDS             complement(5324..>5625)
                     /locus_tag="OO150_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HCR5517770.1"
                     /translation="KILSVSQQSLERYSAVSEKVAAEMATGAIERADADVSIAITGYG
                     GLEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAVRFAHVQLLQLLL"
BASE COUNT         1394 a         1363 c         1457 g         1411 t
ORIGIN      
        1 cagatggatg gcttagaaac cgtgttggct gcgatgtgtg aaccgcaaat cgcgattgtc
       61 tctctgacaa tcaccgaaaa agggtatttc cactctccgg cgaccggaca gttaatgctc
      121 gatcacccta tggtcgctgc cgacgtgcaa aatccccacc agccgaaaac agcaacaggg
      181 gtgattgttg aggcgctggc ccgccgtaaa gcggcaggac ttcccgcatt taccgtcatg
      241 tcatgtgaca acatgccaga aaacggtcat gttatgcgtg acgttgtcac ttcatacgcg
      301 aaagctgttg atgtaaaact ggcacaatgg atcgaagata acgtgacttt cccatcaaca
      361 atggtggacc gtattgtgcc cgcagtgaca gaggatacgc tggcgaaaat cgaacaactt
      421 accggtgtgc gcgatcctgc gggcgttgcc tgtgaacctt tccgccagtg ggtaatagaa
      481 gataactttg ttgccggacg tccagaatgg gaaaaagcgg gagccgaact ggttagtgat
      541 gtgctgcctt atgaagagat gaagttgcgc atgctcaacg gcagtcattc attcctggcg
      601 tatttgggtt atcttgccgg atatcagcac attaatgact gcatggaaga tgaacattat
      661 cgttatgcgg cgtatggctt gatgttgcag gaacaagcgc cgacgctgaa agtgcagggc
      721 gttgatttgc aagattacgc taaccgatta attgaacgct atagcaatcc ggcgttacgt
      781 catcgaacct ggcagattgc gatggatggt agccagaaat tgccacagcg gatgttggat
      841 tctgttcgct ggcatctggc gcatgacagc aagttcgatc tgctggcgct gggcgtcgcg
      901 ggttggatgc gttatgtcgg tggtgttgat gaacagggaa atccgataga aatcagtgac
      961 ccactgttac ctgttattca gaaggctgta caaagtagtg ccgaagggaa agcgcgcgtc
     1021 cagtcattgc tggcgattaa ggcaatcttt ggtgatgatt tgccagacaa tagcttgttt
     1081 actgcaaaag tgacggaagc gtacttgtct ttattagcgc atggcgcgaa agcgaccgtg
     1141 gcgaaatatt ccgtgaagta aaaaaagcaa tgccgccatc ggcggcattc acacacttag
     1201 ttcatgccga gacgaatcag gtccagaggt gccagtttct cttcacaacc ttcaacaact
     1261 acggtttttc cgcggcggat ttgctcagcg gtcagacctt cagtatcaat cgacaggatc
     1321 ttgccagtgt gaccggtgcc gctaaccata acacggctgc cagtggtgat tgcgttacgg
     1381 ttacgatcgt aggtcgtcat aggtgttttc tcctttctga tttacagtcg aagcccacgt
     1441 aacgggcgca atataaatac tcctgtcgag tgaaatattt tttgtttttg atcaagctca
     1501 catctttttt gatgcatgca tgagccgtaa ggcgggggga aaagcctctc atcccggatg
     1561 ggatgagagt aaatgaatta ctcttcgcta aaccagtcgc tgttttcctg gcggatctgg
     1621 ccacggattc gctgatttcc tgcaaatgtt gcgtcattgc acgttctacc gcatcgccat
     1681 cacgtttttg cagggcagag aaaatatcaa gatgctggcg taacagcatt tctggtggag
     1741 aaacatggtc gaaactcata tagcgcacgc gatcaacggt cgctttcagg ttctcaatgg
     1801 tatcccacgc cagttgacag tcggcaatct gcgtcaggag ttgatggaag ttgtcatcaa
     1861 gttcaaaaaa tcatccagtt gcttgtgctc aatggcaatg cgttgctggt gaagattttg
     1921 ttccagttgg tagcactggc tttcggtaat catgctcgcc gcccgacgcg ccaccgcgca
     1981 ctcaatggcc tgacggataa aactgccgtt gcgcacctgg gccatggaaa ttttgttgac
     2041 gtagctgcca cgctgtggac gaatttgaat caggccgttt tccgccagtt taataaaggc
     2101 ttcacgaacc ggctggcgtg acacattgaa acgaacagaa acttcttttt ccgacaacgg
     2161 tgtgcctgga gcaatcaggc aatggacaat gtcgcgacga aggatacgat aaatttgctg
     2221 attgacaggc tgtgtgggat taagttgcgt ttcgacggtc attcgttctt gcttgtgagt
     2281 gagttaactg catgagtcta ccactttttt gtgacaagca ataacccggc agttacgccg
     2341 ggtataaaaa ttactgacgg tggacattca gtccggcata gctttgggta accggcatca
     2401 tttccagggt attgatattg acgtgagcag gcagcgttga cacccaccaa acggcttcgc
     2461 tgacatcttc tggcgtcaat gcaacggtat tttgataggt tttttccgct ttaccgtcat
     2521 cgcctttaaa gcggacattg gaaaactcgg tgccacccac cagacccggt tcgatgtcgg
     2581 tgacgcgcac cgccgtacca tgcaggtccg tacgcagatt caggctaaac tgacgaacaa
     2641 acgctttcgt cgcaccgtaa acgttaccac cggcataagg ccagctacct gccgttgagc
     2701 caatgttaat aatatgacca tgattacgtt caaccatacc cggtaagacg gcgcgcgtca
     2761 tatataccag gcctttgttg ttggtatcaa tcatcgtttc ccagtcttca acgctggctt
     2821 tatgcgcagg ctccatgccc aacgccaggc cggcattatt taccaggata tcaatattgc
     2881 accactcggc aggaagcgat gccagcatct cttcaatagc ggcgcggttg cgaacgtcca
     2941 gttgggcgat gtacagatta tctcccagtt cgtcttttaa ctcctgcaac cgttcctggc
     3001 gacggccagt ggcgataact ttatgccctt gttgaataaa acgacgagta atgcattcac
     3061 caaaacctgc cgttgctcca gttactaaaa cgatcatctc actgttcctc aacgcttttg
     3121 tgtgtcaata acatagcacg tgatgcttaa gagcgggtaa tactggtttg taagattgca
     3181 ctccgttatc agtaagccta cgctatggaa cacctcgcgt tcaggagaag aaaatgacaa
     3241 caatgaatcc tttccttgtg caaagcacac taccgtatct ggctccccat tttgatcaaa
     3301 ttgccaatca tcactatcgc ccggcattcg atgagggaat gcagcaaaag cgggcagaaa
     3361 ttgctgccat cgcgcttaac ccgcaaacgc ctgatttcaa caatactatt ctggcactgg
     3421 aacaaagcgg agaattactt acccgcgtta ccagcgtctt ttttgcgatg actgcggcgc
     3481 ataccaatga tgaattacag cgtcttgatg aacagttttc cgctgaactg gcggaactgg
     3541 ctaatgatat ctatctgaac ggtgaattat tcgcgcgggt agatgctgtc tggcagcgcc
     3601 gtgaatccct ggggcttgat agtgaatcca tccgcctggt ggaggtgatt catcaacgtt
     3661 ttgtccttgc cggagccaaa cttgcgcaag ctgataaagc aaaattaaaa gtactgaata
     3721 cagaagctgc gaccctgacc agccagttta accagcgatt actggcagca aataaatccg
     3781 gcggtctggt tgtgaacgat atcgcgcagc tggcaggaat gagtgagcaa gagattgcgc
     3841 tggcggcaga ggcggctcgc gagaaaggtc tggataacaa atggctgatt ccgctgctga
     3901 ataccaccca acaaccggcg cttgccgaga tgcgcgatcg tgcgacgcgt gaaaaactgt
     3961 ttattgcggg ctggacgcga gcggaaaaaa atgatgccaa tgatacccgc gctatcattc
     4021 aacgtctggt agagattcgc gcacagcagg cgaaactgct tggttttcct cattatgccg
     4081 catggaaaat cgccgatcag atggcaaaaa cgccagaagc agcactcaac tttatgcggg
     4141 aaattgttcc agcggcgcgt caacgtgcta gcgatgaatt agcctccata caggcggtta
     4201 tcgataagca gcaaggcggg tttagcgcgc agccgtggga ctgggcattt tatgccgaac
     4261 aggtacggcg ggagaaattt gatcttgatg aggcgcagct caagccatat tttgaattaa
     4321 acacggtgtt gaatgaaggt gtattctgga ccgcgaatca gctcttcggt attaagtttg
     4381 tcgaacgttt tgatattcct gtctaccatc ctgacgttcg tgtgtgggaa atttttgatc
     4441 ataatggcgt gggactggcg ttattttacg gtgatttctt cgcccgtgat tcaaaaagcg
     4501 gcggtgcatg gatgggcaat tttgttgagc aatcaacgct taatgaaacg catccggtaa
     4561 tttataacgt ttgcaattat cagaaacccg ctgccggtga gcctgcgttg ttactctggg
     4621 atgatgtcat aaccttattc catgaatttg gtcatacgct gcacggcctt tttgcccgcc
     4681 agcgttatgc cacgctttcc ggcaccaaca cgccgcgtga ttttgtcgaa tttccgtcgc
     4741 aaatcaacga acactgggca acgcatccgc aggtattcgc tcgctacgcc cggcattatc
     4801 agagcggggc agcaatgcct gacgaactgc aacagaaaat gcgtaatgcc agcctgttca
     4861 acaaagggta tgagatgagc gaactgctta gcgccgcact tctcgatatg cgctggcatt
     4921 gcctggaaga aaacgaagca atgcaggatg tcgatgattt tgaattgcgg gcgctggtgg
     4981 cggaaaatat ggatcttcct gctataccgc cacgctatcg cagcagttat ttcgcccata
     5041 tttttggtgg cggatatgct gcaggttatt acgcttatct gtggacgcaa atgttggccg
     5101 atgatggtta ccagtggttt gttgagcagg gcggattaac gcgtgaaaat gggcagcgtt
     5161 ttcgcgaggc gatcctttcc agaggtaaca gcgaagatct ggaacgcctg tatcgacaat
     5221 ggcgcggtaa ggctcctcag attatgccga tgctgcaaca tcgtggcttg aatatataaa
     5281 caccaccaat ggctggcctg gagatatcgc caggccagcc tggttatagc agtaattgca
     5341 gcagctggac gtgggcaaac ctcaccgcta aagctaatac cgtttcgcag tcgccagcaa
     5401 aatgcataac cgcagtgtag gtctggcctt taatatgcca cgcgaaccag acggtacccg
     5461 ctggcgtacc atcttcaccg ccctccagtc cgccgtagcc ggtaatggca atactgacat
     5521 cagcatccgc acgctctatg gcacctgttg ccatttctgc tgccactttc tcactcaccg
     5581 cagaatatcg ttcaagagat tgctggctta cgctgaggat tttca
//
LOCUS       DAJTNN010000218         5591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTNN010000218 DAJTNN010000000
VERSION     DAJTNN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.223"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO150_003794"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO150_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5517771.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO150_003795"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO150_003795"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5517772.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO150_003796"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO150_003796"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5517773.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO150_003797"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO150_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5517774.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1391 a         1337 c         1515 g         1348 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagtggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcaccac gttctgtata gtactcggca acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggttgcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgtg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 atgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggt ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tcccgctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tggtggtaca tcctggcgcg ggtaacctgg atggcacggt actgaatgcg
     1261 ttgcttcatt attatccgcc cattgccgat gtaccgcgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaaaggcg tgaaattact cgtgagtatg aagcggtggc aattggtcat
     1441 atgaccgctg gcggtacagt agatgaacca atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atcccatggg caaaccggcg gtcactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaaccggac gtacgcacca gatccgcgtg
     1621 catatggcgc atattaccca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcgtttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ctatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cagaagatat ggtggagttg attgaggtta tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tctacccgta tcggcggcgt gagcttgcca ccttacgact
     1981 ctctcaacct tggcgctcat tgtggtgata acccggagca cgtagaggag aatcgcaagc
     2041 aactttttgc tgcgggaaat ttgccttcaa aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagctcact ggcgaacctt atgcctcaaa acgggcggat gcctcttata
     2161 gcaatacgcc cggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgattcggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgaggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaaa
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gacgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg agtttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaacat tctgttgcag gtactggatg atggtcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatttgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtaa
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
     5341 ctcgtttttg tctataagtt agacggaaaa gactatattt aagatgtttt gcctgaaaaa
     5401 tgagcgaacg ataaagtttt tatatttttc gcttgtcagg ccggaataac tccctataat
     5461 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
     5521 gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt agcgggaaag cgcattatgt
     5581 acaccccgcg c
//
LOCUS       DAJTNN010000219         5547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTNN010000219 DAJTNN010000000
VERSION     DAJTNN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.224"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1810)
                     /gene="dhaR"
                     /locus_tag="OO150_003798"
     CDS             complement(<1..1810)
                     /gene="dhaR"
                     /locus_tag="OO150_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5517775.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVT"
     gene            2038..3147
                     /gene="dhaK"
                     /locus_tag="OO150_003799"
     CDS             2038..3147
                     /gene="dhaK"
                     /locus_tag="OO150_003799"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5517776.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLGNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWDKLGESNVTEQNSNC"
     gene            3119..3751
                     /gene="dhaL"
                     /locus_tag="OO150_003800"
     CDS             3119..3751
                     /gene="dhaL"
                     /locus_tag="OO150_003800"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5517777.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            3762..5180
                     /gene="dhaM"
                     /locus_tag="OO150_003801"
     CDS             3762..5180
                     /gene="dhaM"
                     /locus_tag="OO150_003801"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5517778.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLYTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDYIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1395 a         1416 c         1372 g         1364 t
ORIGIN      
        1 ctgtgacctg ggctgcgtta ataattgcct ctttttcaac ttccgcaaat gacagactgg
       61 tggaaaggcg ggtggcgctg acatcatctg ttgcctgctc ggtaaacaga tgttccggca
      121 aatcactgac gcgaatgcgc ccattatcac tactcagagc aagattctcg atgacgctgt
      181 aaagttcaaa atcgttgcct ggccatgcac aagaaaccag gcgagcgagg gcatcgtcat
      241 caattttcag ccgcgtagag aagcgttttt caagactgcg taatttgtta ttcaccagcg
      301 ccggaatgct gccacgccgc atacgcagag gcgggatggt aatttcaaat gcatgcagcg
      361 cgtaatacag ctggcgacta aaacgatttt gttccaccag cattgcgagg tccgcggtcg
      421 ttgttgcaat cactttgaca tcaattggta ttaaacgccg cgcatccagt cgcgtgataa
      481 caccctgctt gataacctga agcaaagcag actgtaactc caccgccaga tattcaatct
      541 tttcaagaaa cagcgtgccg ccgtgcgcca gttccagccg actcagacgg ccattttcat
      601 tgtccgtacg atcgccacca ataaattctt ccgccagtgc cgcatcacca tataactcac
      661 aattgacggc gatataagga cctgcagcac gctcgctttc attatgaatt gcctgactta
      721 gcagtgcctt gcccacgccc tcttctccac aaagcaggac aggaaagcta ctgcgcgccg
      781 cctggcgacc aaaatgaatc aagcggcggg tttgcggatc gtcctgtggc atatgagcaa
      841 aggtatggct gacttttcct aattgactgg tcatcaactg ccgcatctgt tccacaggat
      901 ggagcaacaa aataaagctg gttccctgcg tttctattat cggtttaagg gttatcaccg
      961 catcaataaa ctgatgctga ctttcaaagg ttgcttctac gtgtttgagc ggatgtgcct
     1021 gttttattgc ttgttgcaat acggcgggta acgttaagag ttcagtgatt gcccgtccct
     1081 gacttgccgt cgcgtcaagg cgcaagaccc gcgccgcctg ggcattgata aattgcaaat
     1141 taccctgctc gtcccagcta atcacgccat catccatact ttctaacagg gcattaagtt
     1201 gatttaaatg acggttagtt tcagcgagca aactgtccgt cagcagtaaa tttccgacct
     1261 cgcgggcgat tgccaacgtc aacggcaaat cagctgcggt agtttgctca accggacacg
     1321 ccagcgctat tgttcccgtc aatcggccct tgctgtcaaa caacggcgtt gcacaaaagg
     1381 cccagttcca gagtgcctgt ttgaaatgtt gatcggccat cgttttcacg gcctgaccag
     1441 agatagccgc taacgatagc gcacaagtac caataattcc ctcggcgcaa tacgtgccgt
     1501 catggaatcc aagtgcactt agctgctgca aggtttgcgg atcgccatta cggctgagaa
     1561 tgcaggcggt ttcatcgagg ataaacagcg cacactctcg cggtgccata tattcccagg
     1621 catcttccag cgcagcctgc ccgagcgtca gcatcgcttt cttacgccga taaatagaag
     1681 caaatgtcac gccctgggcc tgatgtggta cgctccatgt ctcccgtttc atcagcttat
     1741 tgcatcgctc ccaggaggtt gcaattaagg gagatatgcc ccgaccatcg ttgttaaaag
     1801 cgccactcat atcccgcatc ccttatgaca aaaggtgttc cggctcgcaa ttttaagtat
     1861 taatacacgt aaaagtttca atatggaaca ttcattaaag catatgtttc actatgaaac
     1921 ataatttggg ctaattttct tttcccttgc ctgatgcaca atggattcat cggcagtcag
     1981 tggtcgccgt gtcgttgaac atcatccatg ccctaccgta attgctggag caaaataatg
     2041 aaaaaattga tcaatgatgt gcaagacgta ctggacgaac aactggcagg actggcgaaa
     2101 gcgcatccat cgctgacact gcatcaggat ccggtgtatg tcacccgagc tgatgcccct
     2161 gttgcaggaa aagtcgccct gctgtcgggt ggcggcagcg gacacgagcc gatgcactgt
     2221 gggtatatcg gtcaggggat gctttcgggg gcctgtccgg gcgaaatttt cacctcaccg
     2281 acgcccgata aaatctttga atgcgccatg caagttgatg gcggcgaagg tgtactgttg
     2341 attatcaaaa attacaccgg cgatattctt aactttgaaa cagcgaccga gttactgcac
     2401 gatagcggcg taaaagtgac cactgtggtc attgatgacg acgttgcggt aaaagacagt
     2461 ctttatactg ccgggcgacg cggcgttgcc aacaccgtat taattgaaaa actcgtaggc
     2521 gcagcggcgg agcgtggcga ctcactggac gcctgtgcgg aactggggcg taagttgaat
     2581 aatcaaggcc actcaatagg tatcgctctc ggtgcctgta ccgttcctgc cgcgggcaaa
     2641 ccttctttta ccctggcgga taatgagatg gagtttggcg tcggcattca tggtgagccg
     2701 ggtattgacc gccgctcctt ctcttccctt gatcaaaccg tcgatgaaat gttcgacacc
     2761 ctgctgggaa atggctcata ccatcgcact ttacgtttct gggattatca acaaggcagt
     2821 tggcaggaag aacaacaaac caaacaaccg ctccagtctg gcgatcgggt gattgcgctg
     2881 gttaacaatc ttggcgcaac tccgctttct gagctgtacg gcgtctataa ccgcctgacc
     2941 acacgttgcc agcaagcggg attgactatc gaacgtaatt taattggcgc gtactgtacc
     3001 tcactggata tgaccggttt ctcaatcacc ttactgaaag ttgatgacga aacgctggca
     3061 ctctgggacg ccccggtcca caccccggcc cttaactggg ataaattagg agaaagcaat
     3121 gtcactgagc agaactcaaa ttgttaactg gctcactcgt tgtggcgata ttttcagcac
     3181 cgagagcgag tatcttaccg gactggatcg cgaaattggc gacgctgacc acgggctaaa
     3241 tatgaaccga ggctttagca aagtggtgga aaaactccct gctatcgcag ataaagatat
     3301 cggtttcatt ctcaagaata ccggtatgac gctgctttcc agcgtcggtg gtgccagtgg
     3361 tccgctgttc ggtaccttct ttatccgcgc cgcacaggcg acccaggcac ggcaaagcct
     3421 gacactggaa gagctttatc agatgttccg cgatggcgcg gacggcgtaa tcagtcgcgg
     3481 gaaagccgaa cctggcgata aaaccatgtg tgatgtgtgg gtgccggtgg tggaatcgtt
     3541 acgtcagtcc agcgagcaaa atctctctgt tccggtggcg ctcgaagctg ccagtagcat
     3601 cgccgaatcc gctgcacaaa gtacgattac gatgcaagcc cgcaaaggcc gcgccagtta
     3661 tctcggtgaa cgcagtattg gtcaccagga tcccggcgcg acctcggtga tgtttatgat
     3721 gcaaatgttg gcgttagccg caaaagagta aggaattggt gatggtaaac ctggtcatag
     3781 tttcacatag cagccgactg ggagaaggtg tcggtgaatt agcccgtcag atgttaatga
     3841 gtgatagttg taaaatcgcc attgccgcgg gaattgacga tccacaaaat cctattggta
     3901 ccgatgccgt caaagtgatg gaggccatcg aatctgttgc tgatgccgac catgtgctgg
     3961 tcatgatgga tatgggtagc gcattattga gtgctgaaac tgcgctggaa ttgctggctc
     4021 ccaagatcgc cgcaaaagta cgtttgtgtg ctgcgccgtt ggtcgaaggt acactggcag
     4081 caacggtcag cgcggcctcg ggagcggata tcgacaaagt tatctttgac gctatgcatg
     4141 cgctggaagc caaacgtgaa caactgggtt taccgtcctc cgacactgaa atctctgaca
     4201 catgtcctgc gtacgatgaa gaagcccgtt ctctggcggt ggtcataaaa aaccgtaacg
     4261 gcctgcatgt acgtccggcc tcccggctgg tttatacctt atcgacattt aatgccgata
     4321 tgttgctgga aaaaaacggc aaatgcgtca caccagagag tattaaccag attgcgttac
     4381 tacaagttcg ctataacgat acgctgcgcc tgattgcgaa agggccagaa gctgaagagg
     4441 cactgatcgc tttccgtcag ctagctgaag ataactttgg tgaaacggag gaagtcgctc
     4501 cacctactct gcgtcccgtt ccgcctgttt cgggtaaagc cttttattat caaccagttt
     4561 tatatacggt acaggcaaaa tcaaccctga ccgtggaaga agaacaagat cgattacgcc
     4621 aggctattga cttcacgtta ttagatctga tgacgttaac agcgaaagca gaagccagcg
     4681 ggcttgacta tattgccgca atcttttctg gtcaccatac actgttagat gatccggaac
     4741 tgctggcggc ggcaagcgaa ctccttcagc atgaacattg cacggcagaa tatgcctggc
     4801 agcaagttct taaagaactt agccagcaat accagcaact ggatgatgaa tatctacaag
     4861 ctcgctatat tgatgtggac gatcttctgc atcgcaccct ggtccacctg acccaaacga
     4921 aagaagaact cccgcagttt aactcgccaa ctattctact ggcggagaac atttatcctt
     4981 ccacagtact gcaactggat ccggcggttg taaaaggtat ctgccttagc gccggaagtc
     5041 cggtatccca cagcgcccta atcgcccgtg aactggggat tggctggatt tgccagcagg
     5101 gtgagaaact gtatgcgata caaccagaag aaacgctaac gctggacgtt aaaacgcaac
     5161 gtttcaaccg tcagggttaa gccactgttc ggatggcatc gttctgatgt catccggcaa
     5221 tttactcagc actaatggtt tttaactacc tgttatattt ttcaccaatt tatgatgatt
     5281 tttcttcgct aatcacgaac ccaacgccgc tggctggcta ataaacagac gttatttgat
     5341 cactctattt cgccgttatt tatcccttta attccctttt ctaaaatgcc tgacagttcg
     5401 cagaatgaga tttcgatcat gcagctagtg cgatcctgaa ctaaggtttt ctgatacttg
     5461 aataccgttt tattcggttt cgccaaagga gaatgattga tgaaatcccc cgcaccttct
     5521 cgcccgcaaa aaatggcgtt aattcca
//
LOCUS       DAJTNN010000220         5492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTNN010000220 DAJTNN010000000
VERSION     DAJTNN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.225"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(114..3971)
                     /gene="sigA"
                     /locus_tag="OO150_003802"
     CDS             complement(114..3971)
                     /gene="sigA"
                     /locus_tag="OO150_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5517779.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEVGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4332..5336)
                     /locus_tag="OO150_003803"
                     /pseudo
     CDS             complement(4332..5336)
                     /locus_tag="OO150_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5321..5492
                     /locus_tag="OO150_003804"
                     /pseudo
     CDS             5321..5492
                     /locus_tag="OO150_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1367 a         1238 c         1223 g         1664 t
ORIGIN      
        1 ataatgtcag aaaaagaaag ctaataatga gagcaagagc gatatagtct gtcacagggt
       61 aatacatatt ctctccggta gaagaagggc cgcaaacgcg gcccgggctg ttatcagaaa
      121 gagtaacgga agttggcgtt gatcgcgtta tccacgttgt atttaccaaa tgccgatttc
      181 tcaaactcaa gaccaaagcg aagattatcg cgaatttcgg cgttgagacc aacattcatg
      241 agaatacgac cgtctttttc acctttgata cgtttctcac cggacgcatc acgcagtacg
      301 gtttcaccgt tggcaaacag gtcaaactgg tagccaaggc cggcgcgggc tgtgactttc
      361 cagtccttac cggagaagga tttacccaca tcaacaccgg tacgcccaat cagcggatta
      421 aagtccttat ccttcatggt cacgctcatt ccgcgatcct gccagtcgaa ccgtttacct
      481 gatacggccc cataaaccag ttctgcctgc ggctcaatcc aggagtcttc tgttacatga
      541 tagcggtagc ctgcttcagc acccacatac aaggaatgag aactgtagtc ttttgttccg
      601 agcccagcaa aggtcgccga gtactcatta tcatggtgaa catacttact aatcaggtcg
      661 atataggcac cagagtcaaa tatagcggaa gcatacagac ctgccccgac ggacttcgtc
      721 ttgccactga atgcattact gctggcatga ctgtcggtat acgtcatagt cagaccagtg
      781 aaaaggtcaa gtccatccag ctcatgtttt ttatccacac caatctgcac atgggtgtag
      841 ttgtcactgt atccaccaga agctgaacct gctccgctca tgatgcgggc ccatgcaccg
      901 acctcgccat tggtgtcacg cagatccccc attcgtttat tcaggttgtt gacttctgtc
      961 aggaaggctt tgtaattacc ggacatatac gattttgctg cactggaaga tgctgtattt
     1021 tcagccacct tatatccggt cagggtccac gtggtatgtt cctgatcctc tttcgtgctg
     1081 atcacaggag atatgttgct gaaacctatg gcctgagtac tggctttaaa tatctcttta
     1141 ttgcttcctt ttggggctgt tatcaggtca acgtttaatc ctgagttatt atttcccact
     1201 ttctgtatat cgaccgacag actgttattg cttccggtta actgattttt aacaatgagt
     1261 ttatccgatt gatttacaga ctggctcaga tcagcccgca taatcacagc agtattagtg
     1321 gcatccagtg tgtttactgt cacagtatgg aaacccgaat tctctatgtt actaaacaac
     1381 aaaagactgt cgcgggtttt taatgattgc agggctgact gtccgtttac tctccataag
     1441 gtgttataca tgctggctga ggcagcagga gcatcaatac ctccggtata agaagtatca
     1501 aagccaccca gcaaacctgt agccagcggt gtgtgtgtgg ttttgtccag gttatcttct
     1561 ttatactggt caacaccaaa cagaatagat gcttttttgt ctgattttat attaccattg
     1621 acccaggcat aattttgagc ctttaacgtg gcaccatctt ctgtcatatt atatccatct
     1681 gacatataga ttgccgggct gtatttatta ttttctgtag gggtgcctgt taatgtcagg
     1741 gttccggcca gttcaatagc tttatctaca tacatttttc cctgggcggt aacagtagct
     1801 cctttgttga taatcaggtc tgtattaatt aaggataatg caccagtcat agtaacagtt
     1861 gatttatcac caacagttat tttgctgttt tgagcactca gactgccgtt gaaactactt
     1921 tcgcctgctg aatccccgga gcgaagctgt tgtctgaatc caaaaccttc gccagtaatg
     1981 tttcttcctg agaacatgtc aatgtatgca gttttatttc ccagaacaat ttgagagttt
     2041 ttagcctcta tgtgtccttt tacattagca tcccggccta tactcagggt tgcgttttct
     2101 aaatttaatt tccggaaatt aaatttacga ctttcccagt cgggctggga aaaagaggct
     2161 atctgattgt tggacttata ttccgtattg taacgtttat ttacagtgct ttcctggtcc
     2221 tttatggcag cagaatagtc tttttgacat aaaaaaggaa ttggacagtt gttattgcct
     2281 tctttaaata tggcatgttc tgtcgcatgt ccttgtaatg ttaactgtgc attccggaca
     2341 gagatatcgt ttttgatatc gactgagcca tcaaagatca gattggcatt attgttatgt
     2401 tgctgggtag tattgataat atgatttatg cttatattac ctgatacatt cccatggtac
     2461 atatatgcat cagtattagt aagtgataat gttgattgct tcacgtttga gttcgttacg
     2521 gttgttcccg catctgttgc tgcgattttc tgaaatgact ggtcatagcc gtttagatcc
     2581 agtgttccgc catttctggt aaaatagatt tcgccattac cgctttcgct taaagcgtct
     2641 ttggcattta tttttaccgt acctttacct ccggccatgt aaattttatt gaaggctttt
     2701 tcagcactaa ggatgacagt cccattacct attttaaggt tatttccctg tgctattttt
     2761 acatccagag taccactacc tattttatgc agattatctc cggcaacgcc cttaacattc
     2821 cattcaacgg tggtattatt atcaatatct atcccggcac ctttataaga cttatctttt
     2881 ccgtacactt tatatgtctg gttcttatcg aatataagcc cgccaatgcc aagatcggta
     2941 tcctgcttga gcgttaattc ccctcctcct gagacagaca ggtccttatc ttttataatc
     3001 gatgataact cttgtttttt accatccagc tgcatagtgt ttgtgaaatt cttttttaaa
     3061 ttgtttacca gaatattatt aaataacgag tagtttgtcc aggctatatt ggtactactg
     3121 ataaaaccaa ctgtactggt aacaccatgg aaaacccatt tttgttgttc tttatcatat
     3181 agataagaac cagaaccact atcgccaaaa attggtgagt ttttaaaagg ggttctgttg
     3241 gtataaatgt aactcttgtt tttccagtcc agattaaaaa gacttgcgct taacaagtcg
     3301 ggattatagt tttgtccaaa cagatattgt ttgccgttta atatcgttga ggttgttccg
     3361 gcacctgctc tgaagccaat tatttgttcc ttaccgttgt agtttacgcc atatcttttc
     3421 atcgcatctt cgggagaaag tgagaagtcg gcactctccg taacccctgt tgtttccacg
     3481 acgaatttat tcagacgatg aaccgaaaag tcgccagatg atactctgtt acttgcttta
     3541 tatgctgtct gatcccatga ctgtgttgtt attgcatgat gctgtgtacc gttatgagtc
     3601 gcagtaacac tgtacgcacc tccaattgat gttgttgcac ctttgttgga aacagcagaa
     3661 aaatcaggta caggtagttc tggaaaatga aatatctttc catctttcat cattaattga
     3721 acatttgttg cacccggtct gaactctcct ctgttttgag caaggtcaag ataatcgcgg
     3781 gtccatatat tatttatatc cagcattgct gcaaatgctg tttcaccata acttccatat
     3841 attgaggata aaataatacc gaggataact ttctttcttg atgtaccgac actaactttc
     3901 cgggtcagtt cagaaacagc aactaatcca cctgtaatat gactatattt cagtgaataa
     3961 attttattca tacgtgaact ccgatatttc tgagttgtgg ttatgggata gcatttaatt
     4021 acaacgggga actatcagtc atggtacgta actgataagg gtataatgat aatcactgtc
     4081 agttatattt gtatgacaga tgtaacaaaa tgaaaaaaaa gataagcaca agtgattgat
     4141 tggtcagata ttgatcacct ggtgtttgta ttttgacctt tgcgatcaaa atttgatctt
     4201 tgttgtttcc ggtcaatcct ttacaggttt tctgtattat ccgggggagt taagtctgtc
     4261 gatgtccgac agacttttga tgatggaaac aacatgcagc gataatgtgc atgctgtttt
     4321 gcaggtggaa ctcagaaggt gaactggaac tgtgcgaccg caccgtaagt gttgtccgtg
     4381 ccgatcaggc ctgcaacatc aaggtgaacc acatcaaacg gtgagatccc caccccggcc
     4441 gtgaatgcac tgccgtcgtt accggccaga ttctgacggt aaccggcgcg cagctgtgcc
     4501 catttccagg cattaaattc tgcgccaatg gcggcaaact gacggttgct gtcggaggtg
     4561 aaaccgctgg ccggggtcag atccacatca aatgcggtgg tgaacatcgc gttgtgccag
     4621 gagacaccgg ccgtcacctg cgggcggatc ctgaaggttt ccgtgatacc gttcacttct
     4681 tttgtctcaa tactacgggg gatcaggttc tgtgcgacca gtcccagcgt ccagttgtca
     4741 tccaggttcg tactggcacc gatgtcggcg ttgatgccat ttttcgtgtt gtggtagcgg
     4801 tcgcctttga aggcgctgct gtcataattt ctgaccagca cgttgtagtt gaacaggtcg
     4861 acgcgctgga atttgggggg gaagcccaga gaccatttct gcccggccgt ttccagttcc
     4921 gtagccagag caatacccac atcagtgatc agtgccgcgc gggcaaaggc ctgtgatgtc
     4981 aggcggcttt tgtccacctc ctgcccggtg ctgtttgcca cctcttccag gtaagtgagg
     5041 tcagcatcag agactttacc gtcgacactg acggtgccgt gtgatttaac catcagtgcg
     5101 gcagagaggc gatcaccggg aagggccgcc acagcagaaa cgcccagctg tgcggcggca
     5161 tgtgtgttac ggaagtcccg gaggtgttct ttcaggcgtg cggcagcctc aggaacaccg
     5221 tggttacttt cgatggcgcg gtcgaaggcc ttccactcat cactgatccg gtcggcgttg
     5281 tcggtgatat tatccggatt ggagagctgc gcgccgacgg acggaagcag gatgctgggc
     5341 tcatgcgcga cgcaaaatcc acgatgtata tatcagtact aaaagcgcga cggcggaaga
     5401 agctctgaaa ctaatcggtg aactgtacgc catagagcac gatatacgcg ggttaccggt
     5461 gtctgaacgc ctggcggtca ggcaaatgca ga
//
LOCUS       DAJTNN010000221         5430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTNN010000221 DAJTNN010000000
VERSION     DAJTNN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.226"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..915
                     /gene="adeQ"
                     /locus_tag="OO150_003805"
                     /gene_synonym="yicO"
     CDS             <1..915
                     /gene="adeQ"
                     /locus_tag="OO150_003805"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5517780.1"
                     /translation="IPLSLRIGITSGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSH
                     GVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIG
                     EVDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDS
                     VSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYA
                     TAGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMK
                     VCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            968..1420
                     /locus_tag="OO150_003806"
     CDS             968..1420
                     /locus_tag="OO150_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5517781.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRISDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1631..2821
                     /gene="nepI"
                     /locus_tag="OO150_003807"
     CDS             1631..2821
                     /gene="nepI"
                     /locus_tag="OO150_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5517782.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRCYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(2862..3155)
                     /locus_tag="OO150_003808"
     CDS             complement(2862..3155)
                     /locus_tag="OO150_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517783.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3377..4195
                     /gene="nlpA"
                     /locus_tag="OO150_003809"
     CDS             3377..4195
                     /gene="nlpA"
                     /locus_tag="OO150_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR5517784.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLHTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4222..5121)
                     /locus_tag="OO150_003810"
     CDS             complement(4222..5121)
                     /locus_tag="OO150_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5517785.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(5232..>5430)
                     /locus_tag="OO150_003811"
     CDS             complement(5232..>5430)
                     /locus_tag="OO150_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5517786.1"
                     /translation="YFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
BASE COUNT         1298 a         1234 c         1390 g         1508 t
ORIGIN      
        1 attcccttaa gtttacgtat tggtatcacc agcggaattg gattatttat cgccttaatg
       61 ggattaaaaa atactggcgt tattgtcgcc aataaagaca cgctggtgat gattggcgat
      121 ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac cgtgttgtca
      181 tcacgtcatt ttcatgccgc ggtgctggtt tctatagtgg tgacgtcttg ctgtggatta
      241 tttttcggtg atgttcattt tagcggcgtc tattccattc cgcctgatat tagcggcgtc
      301 attggtgaag tagatttgag cggcgcgtta acacttgaac tcgccggtat cattttctcc
      361 tttatgctga tcaacctatt tgattcatca ggaacattaa ttggtgtaac tgataaagcg
      421 ggcttaatag atggtaacgg taaattcccc aatatgaata aggcgctgta tgttgatagc
      481 gtcagttcgg tggcgggtgc gtttatcggc acctcgtctg ttactgccta tattgaaagt
      541 acttctggtg tggcagtcgg tggccgcacg gggctgactg cggttgtggt tggcgttatg
      601 ttcctgttgg ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta cgcaaccgcc
      661 ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc tggcgcgcgt taactgggat
      721 gattttaccg aatcggtgcc tgcgtttatt accacggtga tgatgccctt tactttctcg
      781 atcaccgaag ggattgcact cggctttatg tcgtactgca tcatgaaagt gtgcaccggg
      841 cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag ctctgtttgc actgaagatt
      901 attctggtgg attagtaact ggcgagggtt tccagatatc atgagttctg attacgcagg
      961 agaactcatg atctggataa tgctcgccac gctggcggta gtgtttgtgg ttggttttcg
     1021 ggtgctgaca tccggggcca gaaaagcgat tcgccgtatc agtgatcggc tgaatatcga
     1081 tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag tcagccggtg acgaattttt
     1141 acgttatttg catcgcccgg atgagtcgca cctgcaaaac gccgcgcagg tgttgctcat
     1201 ctggcaaatt gtcattgtcg atggtagcga acagaacctg ctgcaatggc atcggatttt
     1261 acaaaaagcc cgtcttgccg cgccgattac cgatgctcaa gtcaggctgg cgctgggttt
     1321 tctgcgcgaa accgaacctg aaatgcagga tattaatgct tttcagatgc gctataacgc
     1381 gttctttcag cctgccgagg gcgttcactg gctgcattaa aaccgcttcg tcttatcagc
     1441 ctacagaccg cgcctgacct gtcgggtgga taagacgttc acgccgcatc cggtaatctt
     1501 tgccctgttc cgtttcgatc ttaactccag ctcaagcaac accgtaaaag ttgcaaaaac
     1561 gttaaatgca tcacacattt caatgttaaa gtggtcggct tttcccctga aacatgccac
     1621 gggtaacacc atgagtgaat ttattgccga aaaccgcggc gcggatgcca tcacccgacc
     1681 gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc tgtctgatta tcgttgagtt
     1741 tttgcccgtc agtttgttga cgccaatggc ccaggattta ggcatttcgg aaggggttgc
     1801 cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt gccagtttgt ttattaccca
     1861 gacaattcag gctactgacc gctgctacgt tgttattttg tttgccgttt tgctgacgct
     1921 ctcctgcttg ctggtttcct ttgctaactc attcagtttg cttttaatcg gtcgtgcctg
     1981 tctggggctg gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca tgcgtctggt
     2041 gccgccgcgt acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt ctattgcgct
     2101 ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt atcggttggc gcaatgtctt
     2161 taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg attatcaaat cattgccttc
     2221 actgccaggc gaaccctcgc atcagaaaca aaatactttc cgcttattac aacgtccggg
     2281 tgtgatggca gggatgatcg ccatcttcat gtctttcgcc gggcagtttg ctttcttcac
     2341 gtatattcgc ccggtgtata tgaacctggc gggattcggc gtggatggct taacgctggt
     2401 gctgttgagt tttggtatcg ccagctttat tggtacgtcg ctttcgtcgt tcattcttaa
     2461 acgttcggta aaactggcct tagcaggcgc gccgttaata ctggctgtga gtgcgttggt
     2521 actgacgttg tggggaagcg ataaaatcgt tgctaccggc gtggcgatta tctgggggct
     2581 aacttttgca ttggttcccg tcggctggtc aacgtggatc acccgctcgc tggccgatca
     2641 ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag cttgctaata cctgtggcgc
     2701 ggcaatcggc ggttatgcgc tggataatat tggtctgact tcgccgttga tgttgtccgg
     2761 cacattgatg ttgctgactg cattgttggt tactgcaaag gtgaaaatga agaaatcctg
     2821 atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt cttacatatc cgggcattct
     2881 accttaccca acgcttccca ataattgctc tgattattac gttccatcgc cacgatggcc
     2941 tcgcgtatat gttgtttatt gttttcatct gacaacatta acttttccca cgcttcatct
     3001 gataatgacg cctgtggcgt gcaaatttta cgtaaatctt gtaataccgt ggttttctct
     3061 tttgcggact gcagtgagct aaaaggcgac tcggcgtata cgatgcctgg catagcgaaa
     3121 aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt caacctttta caaatgtgta
     3181 tcgaatgaag cataagtgta gtttgctttc atcgctctca tcattctgta cgaaacgcgc
     3241 ttgcgtggtc agtaagaagt gccagacttt atattccact tttattcctt tttattctta
     3301 tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg ctatagtcag gtaatcatta
     3361 ataaaaggat aaaaaaatga aactgacaac acatcatcta cggacagggg ccgcattatt
     3421 gctggccgga attctgctgg caggttgcga ccagagtagc agcgatgcaa aacacattaa
     3481 agttggcgtt ataaatggcg cagaacaaga tgtcgcggaa gtcgctaaaa aggtggcaaa
     3541 agagaaatat ggcctcgatg ttgaactggt aggatttagc ggttcgttgc taccgaacga
     3601 tgcgacaaat catggcgaac ttgatgcgaa tgtcttccag catcgaccct ttcttgaaca
     3661 agataatcag gcgcatggtt ataaactggt agcggtggga aatacgtttg tcttccctat
     3721 ggcgggttat tccaaaaaaa tcacaaccgt ggcgcaaata aaagaaggcg cgacagtggc
     3781 gatccccaac gacccgacca accttggtcg cgcactttta ctgttgcaaa aagagaagct
     3841 aattactctg aaagagggaa aagggttatt acataccgcg ctggatataa ccgataatcc
     3901 tcgtcatttg cagattatgg aactcgaggg ggcgcagctt ccgcgcgtac tggatgatcc
     3961 caaagttgat gtagcgatta tcagcaccac ttacattcag cagacagggc tttctccggt
     4021 gcacgacagc gtatttattg aagataagaa ttcgccgtat gtgaatattt tggtggcacg
     4081 ggaagataat aagaatgcag aaaacgtgaa ggaatttctg caatcttatc aatcacccga
     4141 agtcgctaaa gcggcagaaa ccatttttaa cggtggcgcg gtgcctggct ggtaattctc
     4201 actcatgccg cgccggacga tttatttttc tggccggcga cgggaatcca ttgaaatcaa
     4261 aatcaccgat gacagaatca gcagcgttcc cagccagtcc ggtaatgtga acgtgatccc
     4321 cagcaacagc aaagagagta gcgcgctact taatggttct gcacagctca aaatgctggc
     4381 ttttggaccg ccaattaatt gtgctccttt caggtacaga ctaaatgtca gggacgtacc
     4441 aatgaccacc aaataaaaaa acgccagaat caaactgccg ttaacgacaa agtttgttcc
     4501 ttgtctggca taaaaaggca acagaatcag accgccaatc agcatactcc agccgacgac
     4561 tggtaacgtg ccatagcggg caattagcgt tgagggatag gtggtataga atgcagcagc
     4621 aaatgccgag gcaatgcccc agaacaacgc ggcaggagag agcgataatg acgtcggatt
     4681 accgtgtgtc accaataaaa aagtcccgac cagcgatgtc aaaatagcgc agaaaaccag
     4741 aatgcccggg cgcgatttac gcaccagtga gaaccaggcg acgataatcg tcggtgagag
     4801 gaattgcagc accgttgccg tggctgcgtt cgatttttcg atggttagca aaaaagtgag
     4861 ctgtacagtt agcgcgccaa ccacggaaaa aatcagcagg ctaatggcat ctttatgatt
     4921 gttaataata gaaaagattt tatcgccatg aacaaatgac agcgtcagta gaatcaaacc
     4981 ggcgaatatc aaacgcgtca tagtcaaaaa ctgcgacgac atctggcttt gctccatgat
     5041 gtattgcgcg cagacccctg aacttcccca caacacggcg gcaatcagaa cgttcagcat
     5101 cccctttctg gtggaaccca tttttccctc tgcatactgt tttgtcgttt atgtatgcag
     5161 gcatgatagc aaaaatggcg aggatggctc tgtttgcgcc acgccgattt ttcttagccc
     5221 agactggtga gctaaatatc tttaataaac agcaggcaaa tcatcgcaat acccaacatc
     5281 cctatcgcca gccagaacaa cgcatggtag ctccagattt caaccataat tccgtcaaca
     5341 gagccggcga taatccagcc gacgcgtgaa gtatttgcat ataacgttgt cgcagagcct
     5401 attttttcag gcattaagtc ctgaaaataa
//
LOCUS       DAJTNN010000222         5339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTNN010000222 DAJTNN010000000
VERSION     DAJTNN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.227"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(890..1831)
                     /gene="fabY"
                     /locus_tag="OO150_003812"
                     /gene_synonym="yiiD"
     CDS             complement(890..1831)
                     /gene="fabY"
                     /locus_tag="OO150_003812"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5517787.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1876..2313)
                     /gene="dtd"
                     /locus_tag="OO150_003813"
     CDS             complement(1876..2313)
                     /gene="dtd"
                     /locus_tag="OO150_003813"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5517788.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2310..3183)
                     /locus_tag="OO150_003814"
                     /pseudo
     CDS             complement(2310..3183)
                     /locus_tag="OO150_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
     gene            complement(3177..3776)
                     /gene="yihX"
                     /locus_tag="OO150_003815"
     CDS             complement(3177..3776)
                     /gene="yihX"
                     /locus_tag="OO150_003815"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5517789.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3917..4804)
                     /locus_tag="OO150_003816"
     CDS             complement(3917..4804)
                     /locus_tag="OO150_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5517790.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4794..>5339)
                     /locus_tag="OO150_003817"
     CDS             complement(4794..>5339)
                     /locus_tag="OO150_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5517791.1"
                     /translation="FGIVYISSQLVLSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQ
                     KSLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFG
                     TKVRYTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLI
                     EPTDEQETASYRYIREQSHEN"
BASE COUNT         1443 a         1345 c         1253 g         1298 t
ORIGIN      
        1 tgataaatga agaaaaaccg tattcatcgc cattaccacc accttaccta tattactcat
       61 taatgtatgt gcgaaaatta cgccggaaaa tcattaaaaa aaagcggatt tttgcatgtt
      121 gttttgtttt tgcgaggcgt tttccagaaa taataagagt gttgcagttg tttcatttat
      181 tactcaaaaa tggtttagtt atcgcccagg atgaattatg ggtgctatga atatattata
      241 ttcagagata tttatttgca taatctgaat ggtgtagccg aaaaacgtca gcatcctaac
      301 agcaccagga tttaaggtga aattaatctt tcatcacaga ccggtcagta ctgccaccaa
      361 ccggtcttta cttgctcatc gttataattt tgttggtaac agttcgggaa agttgttttt
      421 ctaaattaac aacagaataa ctttcttgtt taatgagatt aatattggca accattatag
      481 cctccttccc acggtttaag agcaaacata caacataatg attcataaca tacagagcag
      541 taaatgaatg atactgacaa tgatgccagc ccattatctt tgcataatgt ttgtgagcta
      601 tagattttaa aaatatttat gaggaaagta tggcaccaga ggaggttttg actatcaaaa
      661 atcgctcaaa cctgaactaa gatgatgagt taagcattat ctgcgataat tgccactcag
      721 ctctacaaat aatatttaaa tgctcctgaa cagtgatgta gcaagacacg tatggtgcca
      781 acgaagaata aaaatttata gagttcttat tcaatattac cggcatagac agggaatgat
      841 gtaaatgacc ggaatgaatg agagaggcag caccgcccct ctccataagc tactcttctt
      901 cgttcccgcc ctcttcatac gggccaaatg gcttcgcggg cagaacgata tacgtgcctt
      961 caaacactgc acccggcgtc tcgtcgccaa agatttcaac ttgcatctgc acccgtgctt
     1021 ttcgtccgcg cgccagacgg tcgagatcgc cgcttaaggc accaaggtcg gctaccgcat
     1081 gaggtttacc gctaatcggc ttgctgtagc ggatatgcgc atcggccaga ataatcgttc
     1141 cgccgaggtg gcgttcgcgc agcatcagcc agataagccc ccaaccggtg agcgttgcca
     1201 gcgagaataa actcccggca aacagcgtat ggtgcggatt ctgattaccg gtttccggca
     1261 tggtggtgat aaatttttgc ccggtatatt gctgaatgcg cacgcccatt ttttcactaa
     1321 gcgggatgtg ttcgtaccac gcctgttgca gctgcgcgca ccagtcgccg cgatgcaaaa
     1381 tatcatccag agtggcaaca ggtttaatca tcaaaaaatg gcgaatcggc gtggtggttg
     1441 gtgtggtgat ttctccctga ttaacaaacc ccagcttggc gaaaaactcc accgcgtctt
     1501 cacgggcgct acaggtcacg cgcttaacgc cttcctgacg cgccaccgac tccagggtca
     1561 tcgccatcaa cgtccccagc cctttgtcct gcacgtcggg atgaacggcc ataaagcgaa
     1621 tggacgcttc attgtcggca ttaatataca gtcggcctac cgccaccaga ttcccctgct
     1681 cgtcgacgac catctgatga tgcgccatcg catcccacgc gtcgcgttcc gaaccttttg
     1741 gttgatgcag gggcttacgc aacatttccc agcgaaactg atagtaacgc tctaactctt
     1801 cttctgtttg tggaacccga aggtgataca tagcgatact ctctcttgtt tcccgtgaca
     1861 accctggaag ctggctcata cctgcaacca gaatgtcacg gggccatcat tgaccagcga
     1921 tacctgcata tcggcagcga agcgtcctgt ttgcgtgttc atctcttgct ggcggcagcg
     1981 ttcgacgaaa tagtcatata acgcctctgc gcgatccggt gatgcgcctt tggagaaact
     2041 tgggcgcatc ccccgttcgg tatctgcggc gagggtaaac tgggaaacca ccagtacact
     2101 gccgcccgcc tgttgcacgt tgagattcat cttgccttcg gcatcgctaa agatgcggta
     2161 gccgagcaca cgctcgcaca gacggtttgc tttctgttcg tcgtcatcct tttcgacacc
     2221 caataacacc aaaagtcccg cgccaatttc gcccgtcact tctccctcca cggtgacgct
     2281 ggcacgggtt acgcgttgaa ttaatgcaat catggttcgt cgtcttcttc ttgttcagct
     2341 gcttgtttta gtttgcggta ttccccgaga gtgacagtaa tttccgcgcc aagcaagacg
     2401 atacaccatg tccagtagac ccaaacaaag agaatgggga tcaccgccag cacaccgtaa
     2461 atgagctgat atgacgggaa catggtgata taaagcgcga aacctttctt tcctgcttcg
     2521 aacaggagtg cggcgacaaa cgcgccgaca atcgcgtcgc ggttaggtac gcggatggta
     2581 ggaacaatgc tgtacagcaa ccagaaggag atccacgaca acagcaacgg aaaaatacgc
     2641 agcacgttgt cgatgacagt attgagatcg ctcgcccagc gcagggagag caaataggaa
     2701 ctgatcgcca gactggcccc tgccagcagc ggccccagcg ttaaaatcat ccagtacacg
     2761 gcgaacgagt aaatttttgg tcgcgctcgt ttactgcgcc agatggtatt caacgcgcta
     2821 tcgatggagt acatcaacaa taacgcggtg acgatcagcc cgcacgcccc aacggcggtc
     2881 atcttgttgg aattggcaac aaattgttcg atataccgct gaataacatc gccagtagca
     2941 ggcagaaagt tggcaaaaat aaagtgacgt aactgaatgc tgacgtcgga aaacatggga
     3001 aaagcggcga aaagcgcaaa aacaacggca accagcggca ctaatgagag caacgaccac
     3061 ataggcaagg ttacctgcca gggttgtcat gttgtcctca tcaatacgtt gccagagtag
     3121 ttttagccag gcccatagtg gacgggtacg gtgcctggct ttgtcctgaa tggtttttag
     3181 cataacacct tcgcgaaata gtccgggatg gtggttttat ctttcaccag aatactggta
     3241 atgcccagct gattggctcc ttctatatta tcggcgttat cgtcgaaaaa gaccgtatcg
     3301 ctgggtgaaa aaccttccgc ctgcaaaaca tgctggtaaa ttcgtgcttc aggtttgcgc
     3361 atccccagat cttgcgacag atagatatgg tcagcagcat cacgaatttc cgggtattct
     3421 tccggccaga aggtggtatg caggcggttg gtattggaaa gcaccaccac gcgatgcccc
     3481 tgctcacgca gtttatgcat gatggcgatc acttccgggc gcagcgcaac aaacaccgcc
     3541 tgccagccgt gagagaactg ctcgtagctt agcggtagag ccatctcatg acacagcgcc
     3601 tctgcgaacg cttcgtcgct aatttcccca cgctcatgct gatgaaacgc ctcccccata
     3661 tgaaaactct tcttaagcgt agccagtgga acacgcgtta aatcgctcca ggctcccagc
     3721 acacggttaa agtcgatatc gacaatcaca ttacctaaat caaagatata gagcattttt
     3781 gcctcctttc gcgccacaag aaattaactg tagcgggaaa ggatgggttt ggctatgtgg
     3841 caataagggt aatcttgtga tgctgattgc gaaaaagcag agtaatttca ctggcttttt
     3901 cgcaatagat gaatggttaa gattcaggtg taaattgcca acgagtttcg atgaccagcg
     3961 gcctatctgg tgtcaccaat tccggcgtta gatttattgc gttaggtgcc tgagtcagcg
     4021 ggttaataca ggtggcatcg ggctgtttat caaataccac cagactgttt gcagaagaga
     4081 tcatggtcat cgcgagttta ccaggccaca gtaacttaac cttcaccccg ttataaaaac
     4141 caaaacagtc atcccaggga ccggcttgag gagagatacg atttccagtt ggcagttcat
     4201 cactacccgc ttcttcttgc cagtccgcat caaaaagcac ctgcaacgat tctgtatttt
     4261 gcggagtcag ctttttcgca aaccacggat gccagccagc ggaggccggg aatgtatcgg
     4321 catatgaatg gacttccagt tgcaaaacca gcgcatcgtt ctccagcaaa aaagtctgaa
     4381 tcacttcccc ttgccagggc cacggtgaag ccaggggcat acgtaaagtc agggaatctg
     4441 ctgttttgtc gatgatttcc cacgttgagt aacaagccat accatgcaac gcatgtggtg
     4501 gtttattggc gggtaaagaa taacattgcc caccagcatt cagcgtcgca ttccccagtc
     4561 gccctgccca cggcaccatt gggaaacagc catactgaaa agctctgcgc tgcggctgcc
     4621 attggcgtaa tacttcagaa ccaaatgcct ttaacgaggt tatcctggca ccatcatgcg
     4681 gataaatctg caggtgaata ttgccttttt ccagcgtcac taactctcct acaggcccag
     4741 gccatggcgc gtcaaacgcc tgttgtagcg ccaggtaatg ctcgtttttt cgttcagttt
     4801 tcatgagatt gttccctgat gtagcggtaa gaagcggttt cttgttcatc ggtcggctca
     4861 atcagccaga cgcaaacaaa ggaaagaact gagacagcaa ataaaaacag agcgacatac
     4921 cacggttttc ctccccctag cgccagcaaa cttgaggcga taagggggct tagtccgcca
     4981 agaatcgcgg aaaattgata agcaaaagaa aggccggtat atcgtacttt ggtaccgaac
     5041 atgcgggtga aaagtgttgg ctgcacggca aacatcatcg tcggacctaa gttgtagccg
     5101 aggatcatac tgcaccagat gatgagcgta cttttgctat ccagtaataa gaagaaagga
     5161 aacgcgaaca ggacacaaaa tcctgctccc gacaaataca gacttttttg acctatacga
     5221 tctgataacc atccaaccaa tggacaactg aaaataccga tcgccgaggc aatcaacata
     5281 cccgtcaggg gaatatctcg cgacaatact aattggctgg aaatatagac aataccaaa
//
LOCUS       DAJTNN010000223         5198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTNN010000223 DAJTNN010000000
VERSION     DAJTNN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.228"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..107
                     /locus_tag="OO150_003818"
     CDS             <1..107
                     /locus_tag="OO150_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR5517792.1"
                     /translation="EQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(157..1905)
                     /locus_tag="OO150_003819"
     CDS             complement(157..1905)
                     /locus_tag="OO150_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR5517793.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLVLLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPDIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFLITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(1905..2975)
                     /locus_tag="OO150_003820"
     CDS             complement(1905..2975)
                     /locus_tag="OO150_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5517794.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQRPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQMD"
     gene            complement(2965..4416)
                     /locus_tag="OO150_003821"
     CDS             complement(2965..4416)
                     /locus_tag="OO150_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5517795.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4427..4873)
                     /locus_tag="OO150_003822"
     CDS             complement(4427..4873)
                     /locus_tag="OO150_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5517796.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1315 a         1342 c         1312 g         1229 t
ORIGIN      
        1 aagagcaact tcaggacgca aactcaagct ggatggtcga agactctttc ccgagagcat
       61 tacgcgagta caacgaaatg gttgatgact ataatagcct gcgttaaacg tttttgatga
      121 taaatgcagt gtcggatgcg gcgcatctga cacagttcac cccttgtgaa gtaactccat
      181 aaatgcctca tatcctgtta acccggcaat aacctctgcc ggatgctgac acaatatgct
      241 tgccagatag ctaaatattt ttagctcatg acgcgcatcg gcggcggcgc aggcgatcaa
      301 gacatggtta atgacttcgt tattcacctc aactggttgg gcgagggtaa taagaaatcc
      361 acgaaaacgt cgctcctgtt cgctccagca atgtgggatg gcgaggcgat taacaatcag
      421 gttttcacct tcgccttcgc gggcgatgat gcgttgtgct tcatcggcgg taatatggtg
      481 ttgtgcgact aattgcgcac aaatttgccg ggtaatatgt tgccagcttt caccgcgtac
      541 attcgaataa tgaaaacttc cgggggcaga gaaaaaacgc tccggttgct ggcgaataaa
      601 tgccgtcgcc agaaaatgtt ttatttgttc gataccggca gccgtaatga tattttttac
      661 ggtgatgtaa ttatttaccg cgtcttccag taaataatgg ctattgttaa tgatcaataa
      721 cggctcaatc tcttcgcgaa tggcaacaag ttcgcttaag ctacgggcaa taattacccg
      781 acaatttaaa acatcgcgct caatggcgag ctgattaata gtggcaatcg cgttctggtc
      841 cgagagcaaa atgatcggct ggcgttcgtt ttgatgccgc tccagcgcac aggcaaaata
      901 caaaccgatc agatcgctgt cgaaaagggg aatatcgagc tgttcacgca gtagcgtaat
      961 aaagtgcaga ctcatatcaa aggccgcagg ccaggctgct ttcaggttat tcatgctact
     1021 ttggcggtgc tcagcaatcc acaccggtgc ggcaaggcaa cgtaataaat gcccggtaat
     1081 attctcaacc aactgcgtat caataatatt caatgacgcg atgtctggca ccctttgcag
     1141 caaccccagc acatgatcgg ctgaaattac ctgcgcttgc tgatgctgtt tctctatcaa
     1201 acctgtcagg gttcttacag cgttatcacc aagaaacatg ccgctttgtt caacgatttg
     1261 ttttatctcg tcaccgggat attgcggcca ctcatcggtc agttgattac gcagggcgta
     1321 aatcgccaga atcaggctgg agagataatc accctgcatg agcggccagc ggtgttggtt
     1381 gtcgcaggcc gtggataaat gctgaaggtt gtctcgtgtt acgcccgcca gcggaattaa
     1441 aaacggatct ttgcgcagca agttcgccag caagataacg cgcttctctt ctgtctcatc
     1501 aatgaaatgg cccaaaccgg ggcggctggc caggcaacaa gcgcgttcat aacgctgttt
     1561 taaccggggc agacgctctg cgacccacgt ttctggcaaa ttaagcgccg aggcgagttg
     1621 cgcacgggga gtaaaggtat tcagtaataa cagtaccagc agccgatagt cgttatcgtg
     1681 cttttgcagc aactgaaaaa agctgcggcg ctcgaagatt tccagctgat agcccgcact
     1741 accactggcg gaaatgcggg ctttgccgtt aagggtaaag ttgagatagt caatatcacg
     1801 caggatggtc ctgcctgaaa cgccagtctg ttgcgccagc tcaccaggcg tgcgcggctg
     1861 ttgctccagc agatcgacaa tctttaactg gcgttcgttt agcattaatc catctgacgg
     1921 aactggctaa acgcgttgac tttctccgcg gttaaggtgg tcagaaaatc ccgtatcaac
     1981 gtgagcagag catcgtaatc ggcttttgaa atcataccgc tgttagcgtg cagatagcgg
     2041 gttggcaggc acagcgcaac aaccggacgc ccgccgccca tcacgttgta gcgcccgcca
     2101 tccgtcgcac cggttttcat ggtggaaaat tgcagcggta aatcgttatg tgcggcacag
     2161 ctttttaacg ctgctaccag tttctggttg gggaagtagc gcttgtcaaa cagcatcagc
     2221 cccggcccct ggcccagttt cagcgggtat ttaatgttat caatgcccgg aacatcgcct
     2281 gcgacagcgg tatccagcac gatcactaca tccggtttaa tgtgttccgc cgaggtttgt
     2341 gccccgcgta gcccaacttc ttcttccacg ctgccgacgc catacagcgt aatttcggga
     2401 ttattcaccg tctgcaatag ttcagccatc attgcgcagc cgatgcggtt atccagcgcc
     2461 ttgccgacca ctttatcttc gccccagcag gcaaaattgg cttccgggct aataaaatcg
     2521 cccatcgcca caccgcgttt ttccacctca tcgcgactgt ttgcgccaat atcaatgaac
     2581 atctcatcaa atgacagcgg ccgttgcctt tgcttttccg ttaacgcatg aggcgcgacg
     2641 gaaccaatca caccagggat tttcactccc ttgtgtgtgc gtatggttac ccggtggttg
     2701 agcatcgact gattccacca gccaccaatg gtggtgaaac gcagaaaacc gctctcgtcg
     2761 atgtgggtga ccataaagcc gacttcatcc atatgcccga caacggcaac ttttggacct
     2821 ttattcccct tacgggcaac aaagctgccc agaccatcaa aggtgatctc attaacgcaa
     2881 ggttccagcg tgtttatcag aatgtcgcga acttcctgtt catcgccgct gacggcgctg
     2941 gcttcgcaca actgttgcag taactcaatg ttcatggaca agctcctgtt gcgcggcttt
     3001 cgctttgcgg cgtaaccaca atcctttcag gacgataatg aatgaaatgt tcagcgcgag
     3061 gccaatcgcc agcaccagat aaaacgaacc caccggcgac atcaggccga tcagcgggtc
     3121 aaagataccc aaaccaggtg ccagacgttt gattccgaaa gcgatcacca gcacgccggt
     3181 aatgccaccc gccagcgtat tggcggtaat catcggcaac ggagctgcca gagcgtaagg
     3241 tatagccggt tcggttgcga ctgtggcacc gaccacaatg gcactactgg cagcctcttt
     3301 ttcctgtcgg gtaaacagtt tgggcgcaat gaacgtcgca agacccgccg caacgggcgg
     3361 catcaacgcg acaacgccga cgatggcgta ccagtcataa atgtgttttt ccagcagtga
     3421 gaagcagaag aaccaggcgg ttttattgat ggggccgccc atatcgaacg ccagcatcgc
     3481 accgaccaga aacgctgctg caaacttcat tgatggagga atggtattca ggaagtggag
     3541 cagcccgccc atgaggtcgg acatcaccgg cccgataacg tagtaagtga gcacgccgaa
     3601 aaccagcaga gtaacgaacg ggatcagcat tgaaccgagc agcggttgta atgctttgcc
     3661 aaggcgcact ttgcggaacc agaagacgaa atagccaatc gccagcccca gcaccaccgc
     3721 gccgaggaat cccgcacccg actgggtgcc gagcagcgct ttgtcgttcg ccagatagca
     3781 aaccagaaac gccggagcga aagccggttt atcggcaatc gacgaggcaa tataagcgcc
     3841 catgatgggg atcataaagg tgaagccaag gtaaccgatg gattcaacta cccaggtaaa
     3901 cgacggcgcg cctttcgaca tatcggtata cggcaaaccg aactgcacca gcatgttggc
     3961 aagcgccacc aggataccac cgccgatgac aaacggcagc gccgcagaga cgcccgccat
     4021 caggtggctc atcacgctgc cgctttgcat ttcctgtttg ccaagcttca cgccactatc
     4081 tgcggcaaaa agctgcgagt ttgtcggtaa ttcgctgaaa atctggtcga tatttttcaa
     4141 cgcctgggag atggcaatct cataaacttt cttcccggta aaccgcgcgc gatcatcacc
     4201 gctcaggcca cgcccggtag cgagaatgac gtaatcggca gcggcaatct cttcgctgga
     4261 taagcggttt tcaacgccac tggacccttg agtttccact tttatggtat ggccgagaga
     4321 acgggctttc tgttccaggg cttccgccac catgtaggtg tgagcgatcc cggcggggca
     4381 gttggtgatc gcgacaatac gtaaggatga ctccatcgcc acttccttat gagctgaggg
     4441 tttgatttaa caaggcaagc acttggtcgg tatcgccctg ttgcagttga tgaataaatt
     4501 ccttgtgaat aattttgcga cacagtgtgc cgatgatttt gacctgatcc tcttcgccgc
     4561 tttgcggcac gccgaggcag atccagcaat tgacgtcttc gccatcgctg gctttccagt
     4621 caatagcctg cgctttgcgg gcgaataata cgaacggttg tttaacgcag gcgcttttac
     4681 cgtgcggcac ggcgacaccg gaaccaaatc cagtagagtg cattttttcg cgcagcagca
     4741 gggtttgcag gaactggtgt gagtcggtga taaaaccgtt ttgtaacgct attgtcgcca
     4801 actgcttcag aacggaataa gcgccattgc cctgaatatt caggtcaata cagcttgcag
     4861 taagagctgc cattaatcgg actccataaa atatatttta atcaattgta atggtggagc
     4921 gattaagtga tgagaggcga tatgtcggta aacaatgaca atttgagaca gagtgaaagg
     4981 tcagatttgc ggagtaatgc acataatggt tatttaaata aaccacatga atcattaatg
     5041 ggttattcat tatttttgtg atttactcac aatgtatcag ggaaatataa cttaccggga
     5101 gatgtaatat gtttaatttt tcaaatcgaa atttaaaata ttgtgccgga ggcatctccg
     5161 gcacattggg caattacggc aggtaaaaca cttcttgc
//
LOCUS       DAJTNN010000224         5180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTNN010000224 DAJTNN010000000
VERSION     DAJTNN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.229"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..340)
                     /locus_tag="OO150_003823"
     CDS             complement(<1..340)
                     /locus_tag="OO150_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517797.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLC"
     gene            complement(345..995)
                     /gene="hyfE"
                     /locus_tag="OO150_003824"
     CDS             complement(345..995)
                     /gene="hyfE"
                     /locus_tag="OO150_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5517798.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(1007..2114)
                     /locus_tag="OO150_003825"
                     /pseudo
     CDS             complement(1007..2114)
                     /locus_tag="OO150_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(2126..2236)
                     /locus_tag="OO150_003826"
     CDS             complement(2126..2236)
                     /locus_tag="OO150_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR5517799.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(2253..3200)
                     /gene="hyfC"
                     /locus_tag="OO150_003827"
     CDS             complement(2253..3200)
                     /gene="hyfC"
                     /locus_tag="OO150_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5517800.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(3211..5174)
                     /gene="hyfB"
                     /locus_tag="OO150_003828"
                     /pseudo
     CDS             complement(3211..5174)
                     /gene="hyfB"
                     /locus_tag="OO150_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
BASE COUNT         1436 a         1469 c         1357 g          918 t
ORIGIN      
        1 cgcacagtgt aacgggtgaa agctcgccgt gtgccacttc atgacgcatg tagccaatcg
       61 agtaaacacc ggtgagaaag ccaatcacac caagaatggc gaggaacaaa ccgcccagac
      121 catcaatatg aagccacagt cccgcagcga atatttctcc tgcatcagcg gcagtttgga
      181 ccacccagag tgccagaatc agcagcagtg tgatccctaa gctatgtaat acagtcaccg
      241 tacaagtcgc agagagcccc cgtttccggc aggcaaaaca gagcagcgaa aaaagcagcg
      301 gcgtgagcag gagtaaagcg aacatcacag aataactcat ctcattatcc cttcagcgcg
      361 gtcaagttgt tcacgtccag cgtgccgtgg gtacgccata tttttcttgc cagtaacacc
      421 atcacaatga cggcgaagat ggcgtcggta gcgataccta tttccaccag ttccggtgct
      481 cgccaggcaa gaagcgccag caccagatgg gagccgtttt ccatcaggca gtaaccaaaa
      541 atttgccgca ggatattgcg ctggctgaca atgcacagca ggccaagcag aaaatgacct
      601 aacgctaccg ccagcgccgg tttcagcccg gtagccatcg gtagcttcac gggctgaacg
      661 acaaatgcgc aaagcaggac aattaacgcc gcgagcagtg ccatcattgc cggaccgaat
      721 aacgcttttt ccgggatgtt ctggggaata tttcgtgcag cgtaagtcat gattaacggt
      781 accagcagca ctttggtgat aaaggcgctg gcggaccaga tcagcagttg ctctgcggcg
      841 aacaggcacg agagagtggc gaaaatagac accagcacca gcgactggca ggcgtaaaat
      901 ccgcatgaca ggcgatagct tttgacgcta atcacaaaca gcgatgtcag catcatcagt
      961 cccgccagat tatttacgat catagaaccg atcattcatc atctccttac tgcaaccagg
     1021 tcgcggcgat tacgctggaa atcagcgaca tcacaatcag tacaatcaac accaggcgca
     1081 ttgaccctgg cagcggtgcg gcatcggcga cggcctcgct cggtttgcca ggcacaacgc
     1141 gaccaaacca gcgaataaac caggcgaaac tggcgaccga ttcaatcatc agcagaatca
     1201 tggcgggcag caggatccag tactccactg acaacgcaaa accggcggca aacagcggga
     1261 atttactgaa gaagccgttg aacggcggca caccggtaat cgccagcgct gccacgcaga
     1321 aacccacgcc tggcaacggc agggagtgca atacgccacg cagacgcggc aacaagcgcg
     1381 tgccgcagct gtaactcagc gcacctgcta caaggaaaaa caggctttta gcgaacgcgt
     1441 ggttgacgat gtaggcgatg ctgccctcca gtgccagccg cccactttca ccatcgatgc
     1501 ggcgtggaga taggctctga tcggtgttgg cgcttccatt gcgtccggta gccacgcttg
     1561 catcggtagc tgggccgatt tcccccacgc ggcaaacagg atgccgccat aaaccagata
     1621 acgtgcgtcg ccatgtaact cgctcatcgc gctaagcaca aacgttccgg tctgcaaaaa
     1681 cagcgtggcg gcggcaagat acaaccccaa cgaaccgata tgggtgataa gcaacgcttt
     1741 tagtgttgaa cgctgcgctt tatcgctctg gtaataactg atcaacgccc aggagcagcc
     1801 gccctaattt caaaaaacaa caactgaccg agcagcgtcg aggagagtac cagtcccgcc
     1861 atcgcgccga taaacaccag caaaaatgcg taataacgat tcgtgccgtt atgcgggtgt
     1921 tcgcgatttt tatccgtcag ataacccgtg gagtagatcg tgaccagcaa accgagaaac
     1981 accaccacaa acagaatcag cgtactcacg cgatcaatga ccaggccaaa caatgccaca
     2041 tcccccacgt tgaccaacgt caacgtgacg gcaactttat cggcctgata aaacgcggag
     2101 atcagcgaca acatgcacag cgtggtcagt gcggcgaaca aaacccccat tcggcggccc
     2161 gacgttgtgg cgaaaacgaa acgaccagtg cgccgataaa aggcagcatt aacgtcgtca
     2221 gagcaagatt ttccatattc cgtcagtgct ccttacagac cggttaacca gaagacccat
     2281 gcaagcgcag caaggctgaa gccaagccag gtcacatggt gaatgagtaa aaaacgcccg
     2341 cgtgccagcg tgttttctgc gattgaggcc agtacaaaaa tcagcaaaac cttgagcagc
     2401 gtaacgacaa gtgaagtcag caggcaggcg agagaaagtt cttgcgcgcg cccaaagggc
     2461 agaaacaggg ccacaaacag tgctgccatc acgacctgtt tcagccccag cccccatttc
     2521 gctagcgcca gcccggcacc ggaatattcg gtcagcgggc cttcctgtaa ttcctgttct
     2581 gcttcagcaa catcaaaggg aatttttccc atctcaatga agcaggcaaa accacaggcc
     2641 agtaacgcca gtacggtggt tagtggcgag ttccagcccg ttgccagcgt attgctgatc
     2701 atctcgatat gcgtggaacc tgctatcagc gccaatacca gcagtgagag aatgagcatg
     2761 ggttcgacca gaatgccgag cgtcaactcg cgactggcac cgactcccgc aaacgggctt
     2821 ccggtatcca gtccggaaag agcaaagaaa aaacgaaaca gggcaagaag atagataagg
     2881 gtgatcagat cgccgccgcc cgcaaaaggg gaaacggtaa taaaaagtgg tagggccatc
     2941 gccagcacca gcatgctgct gattaatacc cacggcatca ggcggaacat cagacctgaa
     3001 gatataggcg caacttcctg gcgtttaaac agtttgtgga tctcgcgata atcctgccag
     3061 atccccggcc cgcggcggga gtgcatacgc gcgcgtatct gccgggaaat acccgtaaaa
     3121 agcggggtta gcagcagcag aatcacggcc tgtgctaacg caaaaatgac cagatatccg
     3181 tcgcaaagag tttgtctcat ggtgatttcc ttagacggca atagcgatta gcagcacaac
     3241 cagtgcggca actagataca ggcaatagag acgaaagtcg ccgctttgca gatgctgtat
     3301 ttctttggcc agccgttggg tggcgctcac gatggggcgg atcacccgct catcccagaa
     3361 gggttctgtg ctctgagccc tggcggtgac gtgcgcaaga tctttattta gcctcagcgt
     3421 agggtcgagc tgttgtcgta gacgaaataa cgcagagaag accacacgca gcggctgcat
     3481 cacgccattg cctgacggag ccatcgcgtt ttcccagccg tagccgcatg cccaggcatc
     3541 tcctgtgcga cgaaacgctg cgcggcgcga acgacaccac agccagtaaa ggccaggcag
     3601 caaaggcatc gccagtagta acagcaacag taatgaagga gtgacccgtg tatgaaacgt
     3661 gccgggtaca agtgctattc cgctggcgac agtgacggga ggggtattgg taaacgcatg
     3721 ggcaatatgc attatcttcg gtgccagcca actggcacta agcgcaatta atacgcagag
     3781 tgccgcgagc agtagcatgg cgacgatcat cgtatttggc acttcctgtg cctcttcagc
     3841 gtgtgtactg cgcggcgcac cacagaaagt aataccgtac attttgacga agcacattac
     3901 tgccagccca ccagtgactg ccagcattac catagcaata ggacccgcaa gttgtagcgc
     3961 tacggcttcc acacgactta gtgagaacag cgactgccag gtgtaccatt cgctgataaa
     4021 accattcagc ggaggaatgg ctgatatcgc gaggcaacca atcaggcatg ctgcggctgt
     4081 ccacggcatc cgtttcgcca gtgcccccat tttttccatg tcgtgggtat gcaaacgcga
     4141 aataatcgcg cccgcgccga gaaatagcag ccctttgaac agcgcatggt tgagcagatg
     4201 aaacagtgcg ccgagcagtc caaccacggt gagcagcggg tcgtgcagtg acagaccgtc
     4261 catcgccaca ccgactgcca gcaaaataat gccgacgttt tcgacggtac tccaggccag
     4321 cagccgtttg atatcctgtt ccgccagcgc atatagcacg cccaggagcg cggagattgc
     4381 gccgatcgcc atcaccagaa tgccccacca cagaggcaaa cccgtttgcg ccagcagatc
     4441 catcgctact ttcaggatgc cgaaaatacc gattttgacc attacgccag acatcaacgc
     4501 cgaagcgtgc gatggtgcgg cagggtgagc gcgcggcaac cagctgtgca acggcatcat
     4561 cccggctttc gcgccaaaac cgaaaaaggc cagcagaaac atcgccgacg ccagccccgg
     4621 agaaagtgaa agcgtgcgaa actggcaaaa tcgaggctgc cgctttcgcg ccccatcagc
     4681 aagaaggcga tcataatcag caccgatccg gcgtgggcga taaaaaagta gagcatgcca
     4741 gcgttgatcg acgttttatc ctgcctggca atgaccagaa accaggaaga cagcgacatc
     4801 atttcaaaca gcacgatgaa ccaaaaagcg ttgtccatca ccagcagggc aaccatcgat
     4861 gcgatgaaaa gattcataaa gaagcccatc gccgccgcgc ctttgccctc gtattcgcgc
     4921 atataagtca atgaatagag cgaacaaacc accaccagca aggagataac cagcaccata
     4981 aataccgaga ggctatccat tcgcagggtt aaatcggcaa agtcaaaagg ggtggcaaaa
     5041 cgggcgacta aagttacacc agcatgtaat tgcgtaattc cgctgatgat gccaatcacg
     5101 ccgcccacca gcgatgtgat gccggaaagc ttaatagcca gtctgtccag accgagtaaa
     5161 aacagcgaag ccagactggc
//
LOCUS       DAJTNN010000225         5164 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTNN010000225 DAJTNN010000000
VERSION     DAJTNN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.230"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1075
                     /locus_tag="OO150_003829"
     CDS             <1..1075
                     /locus_tag="OO150_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR5517801.1"
                     /translation="VEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETA
                     RLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPV
                     SLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELG
                     QQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPE
                     LFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQA
                     YPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYE
                     GVAMEYAFLVALAQGTLPATPAD"
     gene            complement(1072..1734)
                     /gene="exoX"
                     /locus_tag="OO150_003830"
     CDS             complement(1072..1734)
                     /gene="exoX"
                     /locus_tag="OO150_003830"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5517802.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWSFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(1758..2414)
                     /locus_tag="OO150_003831"
     CDS             complement(1758..2414)
                     /locus_tag="OO150_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR5517803.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLACDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(2516..2746)
                     /gene="holE"
                     /locus_tag="OO150_003832"
     CDS             complement(2516..2746)
                     /gene="holE"
                     /locus_tag="OO150_003832"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5517804.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            2885..3259
                     /gene="yobA"
                     /locus_tag="OO150_003833"
     CDS             2885..3259
                     /gene="yobA"
                     /locus_tag="OO150_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5517805.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3263..4135
                     /gene="copD"
                     /locus_tag="OO150_003834"
                     /pseudo
     CDS             3263..4135
                     /gene="copD"
                     /locus_tag="OO150_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            4148..4489
                     /locus_tag="OO150_003835"
     CDS             4148..4489
                     /locus_tag="OO150_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5517806.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(4871..>5164)
                     /locus_tag="OO150_003836"
     CDS             complement(4871..>5164)
                     /locus_tag="OO150_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5517807.1"
                     /translation="CRMVSRGDYLISAGIRKNSPDGSIHPDGLTKKFVAARKLTGIQF
                     SENPPTFHEVRSLAGRLYKETCGEEFAQRLLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT         1276 a         1228 c         1293 g         1367 t
ORIGIN      
        1 ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa attaaccgca agacccggga
       61 agtcattggt attgcctttg atgatccagc ctatgtgacc tggattgcct acaatccaga
      121 acctgaaacc gcgcgattgc gttatggtta ttcttccatg accacaccag acactttgtt
      181 tgaactggat atggataccg gtgagcgtcg tgtattaaaa caaacggaag ttcctggttt
      241 tgatgcggcg aattaccgca gtgaacacct gtggatagtc gcccgtgatg gcgtcgaagt
      301 tccggtttcg ttggtctacc atcgcaaaca ttttcgcaaa ggacacaacc cgttgctggt
      361 gtatggctat ggttcttacg gcgcaagtat tgatgccgat ttcagtttta gccgcttgag
      421 tttgttagat cgtggctttg tctacgccat tgtccatgtt cgcggcggtg gtgagctggg
      481 gcaacaatgg tacgaagacg gaaaatttct gaagaagaaa aatacgttta atgattatct
      541 tgatgcctgc gatgcattgt taaaactggg ctatggctct ccttcgcttt gttatgcgat
      601 gggcgggagt gcggggggca tgttgatggg cgttgcaatt aatcaacgcc cggaattatt
      661 ccacggcgtt atcgcccagg taccgtttgt tgatgttgta acaacgatgc ttgatgaatc
      721 aattcctctt accactggtg agtttgaaga gtggggtaac ccgcaggatc cgcaatatta
      781 cgagtacatg aaaagctaca gcccgtatga caacgtcacc gcacaggctt atccgcattt
      841 actggtaacg accggtttac acgattctca ggtgcaatat tgggaaccgg caaaatgggt
      901 cgctaaattg cgcgagctga aaaccgataa ccatctttta ttgctctgta ccgacatgga
      961 ctcaggccat ggcggtaaat ctggccgctt taaatcgtac gaaggcgtag cgatggaata
     1021 tgcttttctg gtcgcgctgg cgcagggaac attacccgct acgcctgcgg attaagtatt
     1081 ttccagataa tgtttcagtg ttaaacgcag ctccgggctc atgctgtcca ggttattaaa
     1141 tgaccagcgc agatagcccg gatcgcgttc ggcaacgtcg gaaaccgctt tgccacggta
     1201 tttgccaaag gtgaaggtcg tcatcaacga cggacgtccg gtgatatcgg ccatctgttc
     1261 tgccgtccag ccggaggtgt tcataatatc gataagcaac gcggcggtga tataacagtc
     1321 atacaacgcg cggtgatgat gcaggcccgg cggcgtctgt acattgagct tccgtgtttt
     1381 atataacgcc atattgctgt acttgatccc tggccacaaa cgacgggcca gtttcatagt
     1441 gcaaatccac tcaccgggca tctcaggcag tacgcggcgg tcaaagctgg cgttgtgcgc
     1501 gacataccat tcactaccgt aatagtgtgg gatcacatct tcaatccacg gtttatcggc
     1561 gaccatggct tcggtgatgc gatgaatcgc catcgcttgt ggactaatag gacgatcggg
     1621 gcgcaccagg tggctcatgg ggttgacgat ttttccgtca atgacatcaa cagaggcaat
     1681 ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg ataatgcgca acatggcatg
     1741 ctccaggccg aaaaagccta gcgtaatgga atgatatcgc cttgccaacc ctgtgatgaa
     1801 cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa cgatgagacg accatattca
     1861 tcccatagtg cactgttacc acgggcattt gccatcagca cggcaatcga gtactgatgt
     1921 gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg gttctcccag acattgccct
     1981 gtagtaaaca gtgaacaggt cgggtccata tccatacctt gcggttgttc atcgaccacc
     2041 gtgatggttc tggaacgacg gcccagacat gcaccatgac tctgatggta gatcccaggc
     2101 gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa agcgatcgtt atattcaacg
     2161 ggaaggccgg caatgatggt cattcgccag gtcgttgcgg cataacaaag cgggtctaac
     2221 agtgagagat cgggtggggc aggaagggca cgtcgcgaat aatcacacgc cagtaaagaa
     2281 agcgatggaa aaactaacag ctgacattgt tgcctggcgg cggccctcac aaactccaga
     2341 tgatgagcta cctgttcggt gagtgaggtt ttacgaggct catattgcgc tgctgcaact
     2401 ttccagaacg acataatgac ttccttttca aaatgactgc aaattatggg tgcggtttag
     2461 aaaatactca taatttagca ggcgttatgt aagaaagtgt aactctgtat aagttttatt
     2521 taagtttggg ctcgtaaggt aaacgtgaca gtttgaccga agccaaacgg tgggcaataa
     2581 gcctctcgcg aaaccagctg cgcaaatgtt caggctgttc acgttcaacc gcttcagcga
     2641 tcaccggcat attgtagcgt tctttaaatg ccaccccggc cgccgccaaa tcgacattca
     2701 ctttatccat ttctgtttga tccagtttag ccagattctt cagcattctt aatctcctca
     2761 tcattcgcgg gcaaaagtta ctaagagtga gccaggatgg caagccggaa ttgatgatcc
     2821 tcgactcaga cattagacaa ggttatgctc tgtaaaaaag ttattacaaa aagtaataga
     2881 taatatggct tcaactgcac gctcccttcg ctacgcgctg gcaatcctga ccacttcgtt
     2941 ggtgacacct tccgtctggg cacatgcgca tttaacgcat cagtatcctg cggcaaacgc
     3001 gcaagtgaca gctgcaccgc aggcaatcac tttaaacttc tcggaaggtg ttgaaacagg
     3061 attcagtggt gcaaaaatca cggggccaaa aaacgaaaat attaaaacat taccggcgaa
     3121 gcgaaatgag caggaccaaa agcaactgat tgttccactg gccgattcgc tgaaacccgg
     3181 aacctacacc gttgactggc atgttgtttc ggtggacggg cataaaacga aaggacacta
     3241 cacctttagc gtgaaataaa tcatgctggc gtttacctag atcgcgctgc gatttatcca
     3301 tttcacctcg ctgatgctgg tttttggctt cgcaatgtac ggtgcctggc tggccccctt
     3361 gacgattcgt cgcttgctgg cgaagcgttt tctgcgttta caacagcacg ctgccgtctg
     3421 gagtttgatc agtgccactg caatgctcgc cgttcagggc gggttgatgg gaacgggatg
     3481 gacagatgta ttttcgccaa acatttggca ggctgttttg caaacgcagt ttggtggcgt
     3541 ctggttatgg caaattgttc tcgccctcgt aacgttgatc gtcgccctta tgcaaccgcg
     3601 gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa tttattctgc tggcgggagt
     3661 ggggcatgcg acgctgaatg aaggggtaac ggcgaaaatc catcagacta atcacacgat
     3721 tcacctgatt tgtgcggcgg cgtggtttgg gggattactg ccggtgctct ggtgtatgca
     3781 actcatcaaa ggccgttggc gacaccaggc tattcaggcg ctgatgcgtt tttcatggtg
     3841 cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg cttagtgcat tgttgattac
     3901 cggatttccc cccacgctta cgacttactg gggccaactc ttattgctca aagccatcct
     3961 ggtgatgatt atggtggtca ttgcgctggc taatcgttat gttcttgtac cgcgcatgag
     4021 gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg actaaactcg aatgggcgat
     4081 aggtgcggta gtgctggtga tcatcagcct gcttgcgact ctcgaacctt tttgatggat
     4141 tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta ctcaccagtt ctgcagcggc
     4201 gctggctgca ccgcaggtga ttaccgtcag tcgttttgaa gtgggtaaag acaaatgggc
     4261 gtttaatcgc gaagaggtga tgctgacttg ccgaccgggt aatgctttgt atgttattaa
     4321 tccgagtacc ctcgtgcagt atcctttaaa cgatatcgca caaaaggaag ttgccagtgg
     4381 gaagaccaat gcccaaccca tttcggtgat tcagattgat gatcctaaca atcccggcga
     4441 aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa ctctgttaat tacctaaaat
     4501 agccttttga tttccaataa aaaaaccgcc tcagttcttt caccagaacg ggcggtttta
     4561 acatttcagc tgatgaccac cacgcttttt attgaccatt ttgcacgcaa actggaaaac
     4621 ctggcgtcgt catctattct taaagggcaa ggcaactaag cctgcattaa tgccaacttt
     4681 tagcgcacgg ctctctccca agagccattt ccctggaccg aatacaggaa tcgtattcgg
     4741 tcttttttta attgcatttg aaatcaatta gttgcaaatg tctccccgaa atttactcga
     4801 atttctgtat tccggtcttt tttggttata tcacaaccaa aatacattta acaatccatt
     4861 tacgttaaaa tcagagcagt aagtacgttt tttctctctc atcaagatac atttttgttg
     4921 tcttctccga tgtgtggcca agtagacgct gagcaaattc ttctccacat gtttctttgt
     4981 acaatcgtcc agccaggctt ctgacttcgt gaaaagttgg tgggttttca ctgaactgga
     5041 tacctgttaa ttttctggct gcgacaaatt tctttgtcag gccgtccggg tgaatgctgc
     5101 cgtcagggct gttttttcta atcccggcac tgattagata atccccccta cttaccatgc
     5161 ggca
//
LOCUS       DAJTNN010000226         5142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTNN010000226 DAJTNN010000000
VERSION     DAJTNN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.231"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            95..2092
                     /gene="betT"
                     /locus_tag="OO150_003837"
     CDS             95..2092
                     /gene="betT"
                     /locus_tag="OO150_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR5517808.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2155..3432
                     /locus_tag="OO150_003838"
     CDS             2155..3432
                     /locus_tag="OO150_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5517809.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
     gene            complement(3712..4874)
                     /locus_tag="OO150_003839"
     CDS             complement(join(3712..4613,4613..4874))
                     /locus_tag="OO150_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HCR5517810.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYTSGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRTRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            4941..>5142
                     /locus_tag="OO150_003840"
     CDS             4941..>5142
                     /locus_tag="OO150_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5517811.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVKNANVPKGSFYYYLKSKEDY"
BASE COUNT         1213 a         1227 c         1305 g         1397 t
ORIGIN      
        1 tacgattaac gccgcatagc cagttgaact ttgctacttt gtgagaggta gtaccttcta
       61 tccagtgcga atttaattaa tggaataaat gattatgagt gaaaatgata caatcccaaa
      121 gaagtctaca agtcagatta acaaagcggt attctttaca tctgctttgc taattttcct
      181 tcttgtcgcc tttgccgccg tattcccgga tgtcgccgac aaaaatttta aactacttca
      241 gcaacaaatc ttcacgaatg ccagctggtt ctacatcctt gctgtggccc tgattttact
      301 gagtgtcacg ttccttggac tctcacgcta cggtgatatc aagctgggcc cggaccatgc
      361 gcagcctgat ttcagctacc actcctggtt tgcgatgctt ttttcggcag ggatggggat
      421 cggcctgatg ttctttggcg ttgccgaacc tgtaatgcat tatctttcgc cacccgttgg
      481 cactccagaa accgttgcgg cagctaagga agcaatgcgt ctgacctttt tccactgggg
      541 actgcacgca tgggcaattt atgccattgt ggcgctgatt ctggcgttct tcagttaccg
      601 tcacggtctg cctttaactc tgcgctccgc actctatccc attattggcg atcgcatata
      661 cggacctgta ggacatgcgg ttgatatttt cgctgttata ggcacggtct ttggcgttgc
      721 gacatcactg ggttacggtg ttttgcaggt gaatgccggt ttgaaccatc ttttcggggt
      781 gcccatcaat gaaacggtgc aggtaattct gatcgtggtc atcacggggt tagcgacgat
      841 ttcagtggtg tccggtctgg ataagggaat acgtatcctg tctgaactca atctgggtct
      901 ggctttgttg ctcctggcgc tggtcctgtg tctgggacca accgtgcttc tgctgaagtc
      961 atttgtggaa aatacgggcg gttatctttc ggaactggtg agtaaaacgt tcaaccttta
     1021 cgcgtatgag cccaagtcga gcaactggct ggggggctgg acattactgt actggggatg
     1081 gtggctttca tggtcgccgt ttgtggggat gttcatcgca cgggtctccc gcgggcgaac
     1141 cattcgcgag tttgtcaccg gcgtgctgtt tgttcccgcg ggttttacgc taatgtggat
     1201 gacggtgttt ggtaacagcg cgatctatct cattatgaac cagggggcca cagacctcgc
     1261 caatactgtt cagcaggatg tggcgctggc cctgtttaat ttcctggagc atttcccgtt
     1321 ctcttctgtg ctgtcattca ttgcaatggc gatggtcatc gtcttctttg taacgtctgc
     1381 tgattcgggg gcaatggttg tggatactct ggcatcaggt ggagtggcaa acacacccgt
     1441 ctggcagcga atattctggg cctcgctcat gggcattgtt gcaattgcgc ttctccttgc
     1501 cggagggcta agtgcgctgc aaacggtgac aatagcgagt gcattgccct tctcagtgat
     1561 cttactaata tctatatacg gacttttaaa agctttgcgc cgggatttga ccaagcgtga
     1621 aagcctgagc atggcgacaa ttgctcctac ggctgcacgt aacccaattc cttggcagag
     1681 aaggttacgc aatatcgcgt atctgccgaa gcgatctctt gtgaaacgtt ttatggacga
     1741 cgttatccag cctgccatga cgctggttca ggaggaactg aacaagcagg ggacgataag
     1801 ccacattagt gatgcagtcg acgatcgtat tcgtcttgaa gtcgatttgg gcaacgagct
     1861 gaattttata tatgaagtga ggcttcgcgg gtatatctca ccgaccttcg cgctcgccgc
     1921 aatggataat gatgagcagc agactgaaca acatcgatat tatcgcgctg aggtttatct
     1981 caaagaaggc ggtcaaaatt atgatgtgat gggctggaac caggaacagc tgattaatga
     2041 catactggac cagtacgaaa aacacctgca cttcctgcac ctggttcgtt aatagcaaca
     2101 tgccgtcctg ggggcggcaa ttattatccc ggccgcaata tgagggaatg cagaatgatt
     2161 tcacgctgga aatggatgct gaagcagaca attaaaaaac tatggttcag ggcaacgtta
     2221 ttcgcaattg tcgcgataat aacggccctt ttatcaattc tttttaaatc aatgatacct
     2281 gagtcggttt ccgtgaaggt tggtgcggaa gcagtcgata acattctgaa catactggca
     2341 tcgagtatgc tggcagtgac cacattttcg ctgagtatca tggtcacagc ctacggttca
     2401 gccactacta atgtgactcc cagagctacg cgtttagttg ttgaagacgt caccacacaa
     2461 aatgtactgg ccaccttcat cggttctttt ctcttcagtc tggtagggat tattgccctc
     2521 aatatgggag cttatggaga aagggggaga gtcattttat tcattgtcac actggttgtc
     2581 attgccttaa tcctcatcac attgcttcgc tggatacagc atttgacctc tctggggagg
     2641 gttggtgaga caacggcaca agtagaacag gcggccatcg aaacatttat tgcgagagca
     2701 agaaatccct gtctcggtgg atatccatgg cttgagaaca atgaacagcc gaaaggaacg
     2761 gttgcagttt atccgaagaa gattggctat gttgaatata ttgatatggt gaaacttagc
     2821 aagctgctga ccaatgatcc ccgtcatgta tacctcgtgg cgcagccagg cagtttcata
     2881 catccgtcca tgccagtttt gtacctgagt cagggccagg agtcgtcaat caacaccgat
     2941 ttacttgaga cgattattgt ctcggatgca cgttcatttg ctcaggatcc tcgattttgt
     3001 cttagcgtca tggccgaaat agcctgccgg gccctttccc ctgcagtgaa cgatcctgga
     3061 accgccattg atgtcattgg cagaggtgtt cgtatacttt ccacttacgc gcagaataaa
     3121 tctgatgaaa tagaagtgaa atatccttca gtacatgttg caccacttca gaataacgat
     3181 ctactggaag actttttctc acctgtcgcg cgcgatggtg ccagtatgag ggagattcag
     3241 ataagagtcc ttaaaggact gtcgatgctg agtaaaggtt ggcctgagat atttgctgaa
     3301 gccgcacata ccctggcatt tgaaacatta gagcatgcaa ctcgtgctga ccatatagat
     3361 tctgatagat atctaataaa atcaacttat tataatttat ttagtggcga atattctaat
     3421 aaaaaaacat agctgcagag caagcaattc ggttgttggc aagtcaaggg caaagcattc
     3481 gcgtgagaat gggcgtatgc tttgtgccaa aagcaaacgt tgctaattct gcactgttca
     3541 gcgatggcgc agtagagaca ggcactgcca ctgtccagtc ggcaaaagcg aacactttgc
     3601 cactgcgggg ataaactgat tgaagctctc gcgcaaaatc acttattctt ttgcgttaat
     3661 aaaatgtagg agatgggtca ttgatcctac ccacgtaata tggacacagg cctaagcgag
     3721 gttcttgttt tcaaattgtt ccggactgag gccgccacac caactgtgcc gccgccaccg
     3781 attgtaatca cattcgatat aattaaacac cgttgcccgc attatttccc ggctgataaa
     3841 gtgttctcca tggatacatt ccactttcag cgaatgaaag aagctttcca cgcaggcatt
     3901 atcgtagcag caaccttttg cgctcatact tccacgcaga ttatgccgct tcagttgcgc
     3961 ctgataatct gctgaacagt actggcctcc acggtccgtg tgaacgataa cgttccgggg
     4021 cctcgtacgc cgccatagcg ccatctgcag ggcatcgcag gccagttgcg ccgtcatgcg
     4081 tggcgacatt gaccagccaa taacggcacg tgaccacagg tcaatgacca ctgccagata
     4141 cagccagcct tcatctgtac gtaagtacgt gatgtctcct gcccacttct ggttcgggcc
     4201 actggtgtaa aaatcctgct ccaacagatt ttctgataca ggcaggccgt gtgcgcggta
     4261 gctgaccggg ctgaacttcc gggaggcctt tgccctcagt ccctgacggc gcaggcttgc
     4321 cgccacggtt tttacgttaa aggggtaacc ctgagcacgc agttcatccg tcaggcgtgg
     4381 ggcaccgtaa cgctgttttg accgggtaaa agccgcgagg acaacgctgt cgcagtgttg
     4441 gcggaactgc tgacgcgtgc ttatccttgt ccgccgctga caccacgtat accagccgct
     4501 gcgggccacc cggagcacgc ggcacattgc tttgatgctg aactcagcct gatgtttttc
     4561 aataaagaca tacttcattt caggcgcttc gcgaagtatg tcgcggcctt ttggaggata
     4621 gccagctctt catcccgttc tgccagctgg cgtttgagac gtgcaatctc ggtagacatc
     4681 tccagttcac gttcagaaga cgtctgctga ttttgctgtt tactgcgcca gttgtagagc
     4741 tgtgattcat acaggctgag ttcacgggct gcggcagtaa caccgatgcg ttcagcaagc
     4801 ttcagggctt cactgcgaaa ttcaggcgaa tgctgtttac ggggtttttt actggttgat
     4861 actgtttttg tcatgtgagt cacctctgac tgagagttta ctcacttagc cgcgtgtcca
     4921 ctattgctgg gtaagatcac atgacaggcg aaaatcgtgg ccggggaagg ccgcgaaaga
     4981 cagaaagtac ctatgcagat actcgtaatg atctgatccg cagtggcctt gaactgctga
     5041 cgcagaatgg ttttcttgcg acgggagtcg atgccattgt taaaaatgcc aatgtcccta
     5101 aaggctcatt ttattactac cttaagagta aagaggatta tg
//
LOCUS       DAJTNN010000227         5089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTNN010000227 DAJTNN010000000
VERSION     DAJTNN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.232"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..244)
                     /locus_tag="OO150_003841"
     CDS             complement(<1..244)
                     /locus_tag="OO150_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HCR5517812.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEK"
     gene            complement(641..880)
                     /locus_tag="OO150_003842"
     CDS             complement(641..880)
                     /locus_tag="OO150_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5517813.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(883..1602)
                     /gene="sanA"
                     /locus_tag="OO150_003843"
     CDS             complement(883..1602)
                     /gene="sanA"
                     /locus_tag="OO150_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5517814.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1752..2636)
                     /gene="cdd"
                     /locus_tag="OO150_003844"
     CDS             complement(1752..2636)
                     /gene="cdd"
                     /locus_tag="OO150_003844"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5517815.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2766..3461)
                     /locus_tag="OO150_003845"
     CDS             complement(2766..3461)
                     /locus_tag="OO150_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5517816.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYGSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(3458..3856)
                     /locus_tag="OO150_003846"
     CDS             complement(3458..3856)
                     /locus_tag="OO150_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5517817.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            4095..5045
                     /gene="dusC"
                     /locus_tag="OO150_003847"
     CDS             4095..5045
                     /gene="dusC"
                     /locus_tag="OO150_003847"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5517818.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         1362 a         1267 c         1294 g         1166 t
ORIGIN      
        1 atttctccgt cgggcacact ctggcacaaa cggcaccgag ggcattattt tcgcgaattg
       61 tctcggcagc gcctttaaaa ttacgaaagt agattgagcg aataaatttc cccggatcgg
      121 tctgtgccgg gcaagcctga ctacagggag cgtcgtgaca taataaacag cgagaggctt
      181 ctttaatcgc cagtaacggc gtaaaagccg gagtgagttc atccagataa ttttgttgcg
      241 gcatgtgatc ttccttaatg ctatgagtaa ttaaggcgaa cgctaaatat actcgtcata
      301 cttcaagttg catgtgctgc gtctgcgggt tattcggccc atccatgggc ctcacccctt
      361 atggggccgc tgcaggcagc gttcaaatcc gctccatgcg gatttgtcgc tcaccccagt
      421 cacttactta tgtaagctcc tggggactca ctcacttgtc gccttcctgc aactcgaatt
      481 atttagagta taaatattgc gtgctgatat taaggctatg cgcgtgggga aatttatact
      541 gtgaaaggat tcacaaatag ctgcggttca gaatgttata tccgcagctt attgagtgaa
      601 gtaataatta ttctcaataa ttaacagaaa agagaaaata ttaacgatag aaaataagat
      661 aaacaatcat gccgatagat aaaattccga agccgatata tgccaccata taagcgaaaa
      721 agagtccggg attttttgct cgccagacat acaccggttc cggtgcattt ttaacccgtt
      781 tatcaatcgc gcctgcgcgc cagcctttcc atgcttcgcg gaaacaaaat atcggaataa
      841 ggaaaaaaac ggcaacgaca aaaaactgct gtacatccat aactactttc cttgtttctt
      901 ttgtaattca agtaactgtt cgggtgtgac ggcgggatac ccctgcgcat cttccggcac
      961 ctggtgcata gccggaatag ggaccagcgg ccctaaaaaa cgcggttcac gtttaaaaat
     1021 ataaaggtca gccagcgcac cgaaacgggc ggcaaattcg cgaatacgta ctgacagcat
     1081 atctttcggt gacggtacgg cataacactg agcctgaatc cccatatgca gcgcaataaa
     1141 taatgcccgc tcacagtgga aacgttgggt gataataatg aaatcattag tatcgaaaac
     1201 tttgcgtgta cgcacgatgg aatccagcgt acgaaagcct gcgtaatcga gaacaatatc
     1261 tgatgggtcg acaccagcag cgattaaatc tttgcgcatg gtcatcggct cattataact
     1321 ttgcaatgcg ttatcgccgc tcagtaatag ataatttacc ttaccgctgt tataggcatt
     1381 aatcgctcct tgaatgcggt agcgataata ctgattaatt acgccagtac gataatattt
     1441 tgctgttccg agcaccacac cgacctggcg gtaggggaga tcctgcaatt cgtcgtagat
     1501 ataaggcgcg gttttccagc tcatccagcg atcgaggccg agcacagtca acaacagcaa
     1561 gccgatcagg actaacaggc tgaggaacac gcgctttaac atgcaggaga ctcgatctaa
     1621 ggaaaatttc catcaggcta ctttacccgc tggttttgcg caagaaacgg caaactgttt
     1681 gccgggaatt tcgacaccag gagagggctg gcaagccgga caaggcgttc acgccgcatc
     1741 cggcaccagg cttaagcgag aagcactcgg tcgatactgt gacagccgag agctttcagc
     1801 gttgcggagg tggcatccca ctgaatcaac ggcgcatcgg ctttttctgc cagaaccgcg
     1861 cgctggatat ccgggtaatc ataacccttg agattcaaca gaattaacgc tccctgcaac
     1921 ggtggcagag tcgggttgaa tgcggcgttt tcagcgtagc tgccactgaa aatacgaccg
     1981 tctttacatt ccagcgcgac accgcttggc gacttactgt aaggcatgtg cgaacggttt
     2041 gccgctgcaa tcgccgcctg agaaagcgca tcaccagtca gcgcatagcc gtgatcctgc
     2101 tcgtccatca gcagcgtttt aatctccaga tctttcggcc caaaggcatc cggcagatag
     2161 tcacgcagcg cgtgtgcctc gcggcccggc agatgaatac gcagatccag accgctgttc
     2221 agttcattca taaactgacg gcagtgacca caaggcgtgt agttaacggt gatggctgca
     2281 agcgcttttt caccactcaa ccaggcgtgg ctgatcgcgc tttgttcggc atgaacggtt
     2341 tgctgcattg tcgcaccaat aaattccata ttggcaccga aataccaggt tccgctcaca
     2401 ccgcgcgcaa ttgcgccaac attaaaattc gacaatggcg tacgcgcaca ggccgctgcc
     2461 agcggaagta gtgcgaatgc cagcgcgtct tcgtccagcc ccgttgcgct cttcagcgat
     2521 gagacttgct ccccggtcaa caaagcgggg aagtacttgt ctgccagaat aggttccagt
     2581 gcagattgca agttatccgc aagttgggca aaagcggttt gaaaacgtgg atgcatggcg
     2641 ttgcctcata atcatgtaat gggataacag tttacgaacc cgttatggct ttatatgtga
     2701 tctgaatctc attaattatg caacgcgtga aacttacatc aaaaatgcgc gacgcatcgc
     2761 aaattttagc ccattactgc cagaataatc gggaaaagga acggtgcgat cagcgaagta
     2821 attatcccgc ataacaccag cgctagcgaa ctaaatgcac cttcctgata atccagctcg
     2881 gcgcagcgcg ccgtaccgag ggcgtgcgag gcagtcccca ttgccagacc gcgcgcagct
     2941 ttggtacgaa tacgcatcgc attaagcaat gtatggccaa ataccgcgcc gaggatgccg
     3001 acgaaaatca cgcaaactgc gctgattgcc ggaataccgc caatactgcc gccaaccgcc
     3061 attgcaatag gcgtggtgac tgatttcggc aggattgacg cggcgatttc cggtgaagcg
     3121 cccatcaaca atgccacgga agtccccgtc accatcgcaa ccacgctgcc gataaaacag
     3181 atggtgatga tcgatttcca gcgcgcgcgg atctggtgta gctgctcata taaaggatag
     3241 gccagcgcga cgaccgccgg ttgcagcaga tcgtttagca cttcgctacc tttaaagtag
     3301 ctgccgtaag agatgccggt cagcattaaa aaagggatga tcaccaccat tgctaccagc
     3361 aacgggttaa gcaagggaaa cttataccgt gccgccagtt tgcgggcggc aaaaaagacg
     3421 atcaatgtca gtggtaatga ccaccagata ttagccatca ttcttctgat cctttctgac
     3481 ctacgacttt acgttcaccg tgcaccagtt gcgaactcca gctcaccacc agaaaaacca
     3541 ccagtgtact gacagcacag gaaaccacta ccgggccaaa ctgtgcgcgg agcaaatcaa
     3601 aatattgcat gacgcctacg ccaatcggca caaacaatag cgccatatag cgaatcagta
     3661 cgtagcaccc cggattgacc cattttgccg gaagaatttg caaggccagc aggacaaaca
     3721 ggatcagcat cccgatgatg ctgcccggaa tggttaccgg tagcagggaa gcaatgaaaa
     3781 tgcctgcata caggcaggca taaatcagga cgaaagcgcg taaatattgc cagataatgt
     3841 tcagtgtctt actcataatc aacaccccag atgagatgaa ttcatcatac aattaattcg
     3901 taaaacgtgc taccgatcac attaagaaat cagcgcttat tttttcgctc cccggcatta
     3961 cgcaatgcgc aacgaactca ggcatacatc aagcatagag agggattaca gtttgcagca
     4021 atccctcttt gcagaccttt aatccgttga ccataggcgc taacatagcg cctcattttt
     4081 ttgcgggtga agatatgcgt gtgttactgg ctccgatgga gggtgtgctt gactctctgg
     4141 tgcgtgaatt gctgaccgaa gttaacgact acgatctgtg catcaccgag tttgtccgcg
     4201 tggtggatca actgctgccg gtaaaagttt ttcatcgcat ttgccctgag ctacaaaacg
     4261 ccagccggac accatccggt acgctggtgc gcgtgcagtt gttgggtcag ttcccacaat
     4321 ggctggcaga gaatgccgcc cgtgcggtgg aattaggttc ctggggcgtg gatctcaatt
     4381 gcggctgccc gtcgaaaacg gtcaacggta gcggtggtgg ggcgacgtta ctcaaagatc
     4441 ctgaactcat ctaccagggt gcaaaagcga tgcgtgaagc tgtaccggcg catttgcccg
     4501 tcagcgtgaa agtgcgtctg ggctgggaca gcggtgagaa gaaatttgaa atcgccgatg
     4561 cggttcaaca ggctggtgct acggagctgg tggtgcatgg acggacaaaa gagcagggtt
     4621 accgcgcgga gcatattgac tggcaggcga ttggcgagat tcgccagcgg ctgaatattc
     4681 cggtgattgc caacggtgaa atctgggact ggcagagtgc gcaacaatgt atggcgatca
     4741 gcggctgcga cgcagtgatg attggtcgcg gggcgctcaa tattcccaac ctgagccggg
     4801 tggtaaaata taacgaaccg cgaatgccgt ggccggaggt ggttgctttg ctgcaaaaat
     4861 atacccgtct ggaaaagcag ggcgataccg ggttatatca cgttgcgcgg attaaacagt
     4921 ggttgagtta tttgcgtaaa gaatacgatg aagcaacgga attatttcag catgttcggg
     4981 tgttgaataa ttcccctgat attgcaaggg ctattcaggc aattgatatc gataagctgc
     5041 gataatagtg gaatcgcagt ttatcagaga caatgaatcg cttattcag
//
LOCUS       DAJTNN010000228         5015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTNN010000228 DAJTNN010000000
VERSION     DAJTNN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.233"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1001
                     /locus_tag="OO150_003848"
     CDS             <1..1001
                     /locus_tag="OO150_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCR5517819.1"
                     /translation="ADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFD
                     ETLKPFVRDASGSRLKDLPKPNKSDDESRSNDAVNRYKLLKKDARIVAAQQVARQESA
                     MCLRRRWSPENFKLFLVEHPLVRHLTRRLIWGVYSAENQLQACFRVAEDNSYSTADDD
                     LFTLPEGGISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTGAERNAS
                     ELTRWAGRKCPSGRVMGLANKGWIKGTPQDGGWIGWMIKPLGRWSLVMEIDEGFAVGM
                     SPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVIDAITASELINDIEALFE"
     gene            1011..1253
                     /locus_tag="OO150_003849"
     CDS             1011..1253
                     /locus_tag="OO150_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5517820.1"
                     /translation="MDKELPWLADNAQLELKYKKSKTPLSHRNWPGEPVSVITESIIQ
                     TLGDELLQKAEKKKRLSGVMKIFHWSGSPPSRRPST"
     gene            2777..4642
                     /locus_tag="OO150_003850"
                     /pseudo
     CDS             2777..4642
                     /locus_tag="OO150_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            4704..>5015
                     /locus_tag="OO150_003851"
     CDS             4704..>5015
                     /locus_tag="OO150_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517821.1"
                     /translation="MIVQKELVAIYDYEVPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDERKLRGESPEPVPVIFNCELQNIFNQ
                     LI"
BASE COUNT         1293 a         1270 c         1284 g         1168 t
ORIGIN      
        1 ttgccgacat tgccgagagc cgcgaactca cggtggcgga gcttgaagat cggttagcac
       61 cggatctcgg tctggatgat aacggttcgt tgctgctgga tttcggccca cgccagttca
      121 ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga cgcttccggc agccgcctga
      181 aagacctgcc caaaccaaac aaaagcgatg atgaatcgcg gtcgaacgat gcggttaacc
      241 gctacaaatt gctgaaaaaa gatgcgcgta tcgtcgccgc ccagcaggta gcaaggcagg
      301 aatccgccat gtgcctgcgt cgccgctggt cgccagaaaa ctttaagctc ttcctggttg
      361 agcatccgtt ggttcgccac ttaacccgcc gtctgatttg gggcgtttat agcgccgaaa
      421 accagctaca ggcgtgcttt agggtggcgg aagataacag ctacagcacc gctgacgatg
      481 atcttttcac cctgccggaa ggcggtatct ctatcggcat tcctcacgtt ctggaaatat
      541 caccgacgga tgctgccgcc tttggtcagc tttttgccga ctacgaactg ctaccaccat
      601 tccgtcagct tgaccgtaac agttacgccc tgacaggagc cgagcgcaac gccagcgaac
      661 tgacccgctg ggcaggcaga aaatgcccga gcggtcgggt catggggctg gcgaataaag
      721 gctggataaa gggcaccccg caggatggag gctggatcgg ctggatgatc aaacccctgg
      781 gccgctggtc gttagtcatg gaaatcgatg aaggttttgc ggtaggcatg tcgccagccg
      841 aactcagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa gcagaaagct
      901 atggctgggg gagtaattca acacaggaag cgcagttctc cgtaatcgat gccatcaccg
      961 ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc atggacaagg
     1021 aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa agcaaaacgc
     1081 cgctcagtca tcgcaattgg ccgggcgaac cggtgtctgt tatcactgaa agcatcattc
     1141 agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaagattg tctggcgtta
     1201 tgaaaatttt tcactggagt ggcagtccgc catcacgcag gccatcaact tgatcggcga
     1261 acacaaaccc tcagtcccgg cccggacaat ggcggcgcta gcctgtatcg cgcaaaatga
     1321 cagccaacag ttgctcgacg aaatcgtcca acaagagggg ctggaatatg cgactgaggt
     1381 ggtaattgca cgcctgttta ttgcgcggtg ttatgagagt gatcctctgg tagtgacatt
     1441 gcagtatcag gacgaggatt atggctatgg ttatcgctca gaaacctata acgaattcga
     1501 tctccgactg cgtaagcatc tctctctggc agaggaaagc tgctggcagc gttgcgccga
     1561 caaactcatt gccgcactac caggaataac caaagttcgc cgccctttta ttgcgctgat
     1621 cctcccggaa aaaccagaaa tagccaatga gttggtaggc cttgaatgcc cgcgaactca
     1681 ttttcattct aagaagtggt taaaagttgt tgctaatgac cccacagcgg tgagaaaact
     1741 cgaacactac tggagccagg atatatttag cgatcgagaa gccagctaca tgtcgcatga
     1801 aaaccacttc ggctacgcgg cctgcgccgc ccttttgcgc gaacaaggac tggcagccat
     1861 tccgcgcctc gcgatgtatg cccataaaga agattgcggc agtctgctgg tacaaattaa
     1921 ccatccgcaa gtcatccgca ccttgctact ggtggctgat aaaaacaaac ccagcctgca
     1981 acgtgtagct aaataccata aaaacttccc ccatgcgacg ctcgccgcac tggcagaact
     2041 gctggcgtta acagaaccac cagcccgccc tggttatcca atcatcgaag acaaaaagct
     2101 gcctgcacag caaaaagcac gcgatgaata ctggcgtacg ctgttacaaa cgctgatggc
     2161 atcgcagcca caactggcag aagaggtgat gcagtggtta agtactcaag ccagggcagt
     2221 gctgaatagt tatttatcgg caccgcccaa accggttatt gatagtaccg ataacagcaa
     2281 tctgcctgaa atcctcgttt cgctaccgtg gcgtagtaag aaaaaaatga cagctccacg
     2341 tcttgatttg gcaccgctcg aattaactcc gcaagtttac tggcaaccag gcgaacaaga
     2401 gaggcttgcc gccactgagt ctgcccgtta tttcagcacg gaatctcttg cgcaacgcat
     2461 ggaacaaaaa agtggacgag ttgtattaca ggaactgggt tttggggatg atgtatggct
     2521 gtttctgaat tatatactcc ccggaaaact ggatgctgca cgcaattcac tcattgttca
     2581 gtggcattac taccaggggc gggttgaaga gatcctgaat ggctggaact ccccggaagc
     2641 acaattagca gaacaggcgc tccgcagcgg tcacatagaa gcgttaatta acatatggga
     2701 aaatgacaac tactcacgtt atcgtccgga aaagagtgtc tggaacctgt atttattggc
     2761 acagttgccg cgtgagatgg ctttgacctt ctggctgcgt atcaatgaga aaaagcatct
     2821 gttcgcgggt gaggactatt ttctcagtat cctcggattg gatgcgctac caggtctgct
     2881 gttggctttt tcacatcgtc caaaagaaac atttccgtta attttaaatt ttggcgcaac
     2941 agaactggcg ctgcctgttg cccgcgtctg gcgacgtttc gcggcgcagc gtgatctggc
     3001 tcgccagtgg attttacaat ggccggaaca tacggctagt gcacttatcc ctcttgtctt
     3061 taccaaaccc agcgataata gcgaagccgc attacttgcc ctgcgtttac tgtacgaaca
     3121 gggacatggc gaattgctac aaaccgtggc aaaccgctgg cagcgtacag atgtatggtc
     3181 tgccctggag cagttgctta aacagggtcc aatggacatt tacccggcac gcattccaaa
     3241 agcccctgat ttctggcatc cggcaatgtg gtccaggcct cgacttatca ctaataatca
     3301 gcctgttacc ggtgacgctc tggaaattat cggcgaaatg ctgcgcttta cctagggggg
     3361 acgtttttat agcgggctgg aacaactgaa aacgttctgc cagccacaaa cgctggccgc
     3421 ttttgcctgg gatctcttca ctgcctggca acaagcgggg gcccccgcaa aagacaactg
     3481 ggcatttctg gcattacgtc tctttggtga cgaaagcacg gcacgggatc tgacgacaca
     3541 gatcctcgcc tggccacaag aaggcaaatc tgcccgtgct gtcagtggtc tgaatatact
     3601 taccctgatg aataatgata tggcgctgat gcagctgcat catgtatcac aacgggcgaa
     3661 atcacgcccc ttacgtgaaa acgcagcgga atttcttcag gtggtcgcag aaaatcgcgg
     3721 gctaagccag gaagagttag cggacagatt agtcccaacc ctgggccttg atgatccgca
     3781 ggcgttgagt tttgattttg gtccccggca gtttaccgtt cgcttcgatg aaaacctcaa
     3841 cccggttatc tttgatcagc aaaacgttcg ccagaaaagc gttccccggt tgcgcgccga
     3901 tgacgatcaa ctgaaagcgc ccgaggcact ggcccgacta aaagggctaa aaaaagatgc
     3961 tactcaggtg agcaaaaacc tgctcccgcg tcttgaagct gccctacgta ccacccgacg
     4021 ctggtcgctg gcagattttc attctctgtt tgttaatcat ccctttaccc gtctggttac
     4081 ccagcgatta atatgggggg tttatccggc aaatgaaccg cgttgtttac tcaaagcctt
     4141 tcgtgtggcc gcagaggggg agttctgcaa tgcgcaagat gagccaattg acctgcctgc
     4201 ggacgctctg attggcattg cccacccgtt agaaatgaca gtagaaatgc gcagtgaatt
     4261 tgcacagctt tttgccgatt acgaaattat gccgcctttt cgccaattgt cgcgccgcac
     4321 ggtgctgctc acacctgacg agtcaaccag taacagcctg actcgctggg aaggtaaatc
     4381 cgctaccgtt gggcagctta tgggaatgcg atacaaaggc tgggagtcag gttatgagga
     4441 cgcatttgtc tatgacctgg gtgagtaccg gctggtcctt aagttttcac ccggttttaa
     4501 ccactacaat gttgatagca aagcgctaat gagcttccgt tctcttcgag tgtaccgtga
     4561 caataaatcc gtcacttttg ccgaacttga tgtgtttgat ttgagtgagg cgttaagcgc
     4621 acctgacgtc attttccatt aaaaacacag cgggcagtga tgcaactgcc cgttatcaac
     4681 acgacttacc agcggggaaa gcgatgatcg tgcaaaaaga gctggttgct atttacgatt
     4741 atgaggtccc tgtacctgaa aatccgtttt ccttcagact tgagatccat aaatgctctg
     4801 aattatttac aggttccgtc tatcgactgg aacggttccg gctacgtcca acatttcatc
     4861 aacgtgatcg agaggatgtt gacccgctaa taaatgatgc gttgatttat ataagagatg
     4921 agtttattga tgagcggaaa ttacgaggtg aatcacctga acctgtacca gtaattttta
     4981 attgtgaact acagaatata ttcaaccaac taata
//
LOCUS       DAJTNN010000229         5002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTNN010000229 DAJTNN010000000
VERSION     DAJTNN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.234"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1151
                     /locus_tag="OO150_003852"
     CDS             <1..1151
                     /locus_tag="OO150_003852"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5517822.1"
                     /translation="DEQEPNEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKY
                     GGWRNRKLIDFFIRFASTVFTCYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSP
                     EEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCMIAMYPIYPLTCVPNDMM
                     MATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSY
                     YMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPL
                     FIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLV
                     SAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(1208..1426)
                     /gene="marB"
                     /locus_tag="OO150_003853"
     CDS             complement(1208..1426)
                     /gene="marB"
                     /locus_tag="OO150_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5517823.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1458..1841)
                     /gene="marA"
                     /locus_tag="OO150_003854"
     CDS             complement(1458..1841)
                     /gene="marA"
                     /locus_tag="OO150_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5517824.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(1861..2295)
                     /gene="marR"
                     /locus_tag="OO150_003855"
     CDS             complement(1861..2295)
                     /gene="marR"
                     /locus_tag="OO150_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5517825.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2507..3172
                     /gene="marC"
                     /locus_tag="OO150_003856"
     CDS             2507..3172
                     /gene="marC"
                     /locus_tag="OO150_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5517826.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3197..4387)
                     /gene="ydeA"
                     /locus_tag="OO150_003857"
     CDS             complement(3197..4387)
                     /gene="ydeA"
                     /locus_tag="OO150_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5517827.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4537..>5002)
                     /gene="yneK"
                     /locus_tag="OO150_003858"
     CDS             complement(4537..>5002)
                     /gene="yneK"
                     /locus_tag="OO150_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5517828.1"
                     /translation="EVHRWFVNKDPNLITAILDITDGELKEDLLQSLMDSLGSNKHVL
                     PEVCICFLSLLAEQESPHFQDFFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKI
                     IKHMARRTLKLFVKNETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
BASE COUNT         1337 a         1133 c         1221 g         1311 t
ORIGIN      
        1 gtgacgaaca ggagccgaat gaagagggtt tacaatttta tgatgatctg ttcgatgaat
       61 gccttaagca gggaatggaa cctgtggtga cgctttcaca ttttgagatg ccttatcatc
      121 tggtgacaaa atatggtggc tggcgaaacc gtaaactgat cgactttttt atccgcttcg
      181 catcaacggt cttcacgtgc tataaagaaa aagtaaagta ctggatgacg tttaacgaaa
      241 tcaataatca ggtgaatttc agcgaaagcc tgtgtccatt tactaattcc ggtatcttgt
      301 attcgccaga ggaagatatc aatgagcgcg aacaaataat gtaccaggcg gtacattacg
      361 agttagttgc cagtgccctg gcggtacaga ctggaaaatc gatcaatcct gaatttaaca
      421 ttggctgtat gatcgccatg tatcccatct atcctctgac gtgtgtaccc aacgatatga
      481 tgatggccac gaaagcaatg catcgtcgtt actggtttac tgatgttcat gctcgtggat
      541 attatccgca acatatgctg aattactttg ccaggaaagg attcaacctc gatatcacac
      601 cagaagataa cgcgattctt gccagtggtt gtgtcgactt tatcggcttt agctactaca
      661 tgtcttttac gacacaattt tcgcccgata acccgcaact ggattatgtt gaaccacgag
      721 atttggtcag caacccttat atcgatacat ccgaatgggg atggcaaatt gatccggcag
      781 ggctacgtta ttcactcaac tggttctggg atcatttcca gttgccgctg tttattgtcg
      841 aaaatggatt tggtgcggtt gaccagagac aagctgacgg cacggtgaac gatcactatc
      901 gcattgatta ctttgcttcc catattcggg aaatgaaaaa agccgtagtt gaagatggtg
      961 ttgacttaat tggctacacc ccgtggggct gcattgacct ggtttctgcc ggaacagggg
     1021 aaatgaaaaa gcgctacgga atgatttatg tcgataaaga caacgaaggg aagggaacgc
     1081 tggaacggat acgtaaagcg tcgttttact ggtatcggga tctcatcgcc aacaatggcg
     1141 aaaatatttg agacttgagg aagatattac ggagatgtca ggctctgtga tcagagcctg
     1201 acaataacta catagcgtgt tgattataat agggcacgcc gagcgcatcc gacttatcac
     1261 tgccagtacc catgtgattt aaatcaaacg gtggttgttc ctgcgatggg ggaacaacca
     1321 cgacattggc acaagaagta acaactggct gcgtggtttg ttccgcaacg ccctgcgcgg
     1381 aaaagagaat aagcgcagct gctattgcgg atgaaagtgg tttcatgatt gcctcagtga
     1441 cgttgtcacg ttatcaacta gctattgtaa tgatttaatg gatgtaaaaa gcgcgattcg
     1501 ccctgcatat tggtcatccg gtatttatgt ggcggaacat caaagtaatt tttgaaggtt
     1561 cgggtcagag tttgttgcga ctcgaagcca tatcgttctg ccagatagag tatcggctcg
     1621 ttactttcct tcagcttttg cgcgatttcc gtcatcttac ggctgcggat gtattggcct
     1681 aatgaatgac cggtttcttt tttaaacatc cgttgcaggt gccatttaga gtaacctgaa
     1741 cgctctgaca ctttctccag tgacagtggc gattccaggt tgtcctcgat ccagtccaaa
     1801 atgctatgaa tggtaatagc gtcagtattg cgtctggaca tcgtcatacc tcttttttgt
     1861 ttacggcagg actttcttaa gcaaatgctc aagtgttgcc acttcgtccg ccgtcaggtt
     1921 ttttgttaat tcttgatgca ggtcctggcc aactaattga tggcattgtt cgcatattgc
     1981 cgcgccgctg gtggtaagtt ttaccagtac gccgcgctta tcattcgggt tcggcaacct
     2041 ttcaacccag cctttacaga ccaggcgatc cagcatacgg gtcagtgctc caaggtcgac
     2101 agacaacact tttttcagtt caaccggagt aatacacgcc gcgcagcgga tagagcagag
     2161 caccttaaac tgtgccgcgg taatatccag cggagacaga tactcgttaa gcagacgatc
     2221 tttcttctga ttaaccatat ggattaagcg acccaatgga ataatttcat tgaacagatc
     2281 gctggtactt ttcacattag ttgccctggc aagtaattag ttgcaggggg taatattgcc
     2341 caggcaagta taagtcaaat aaatgaattg accgatgcca cgttttgcta aatcggttca
     2401 agttttggtc aatgcgatgc aaggttagct aaagagtgac actttatcct tgtttacccc
     2461 ttatactttt cgctgataat ccagatacac tggataacaa ccaccaatgt tagatttgtt
     2521 taaagcaatt ggcctggggc tggtggtgtt actgccgtta gctaacccat taacaactgt
     2581 cgcgttattt cttggcctgg caggcaacat gaacagtgcc gaacgtaatc gtcagtcgtt
     2641 gatggcctcg gtgtacgtat ttgccatcat gatggtggcg tattacgccg ggcaactggt
     2701 gatggataca tttggcattt cgattcccgg tctgcgaatt gcaggcggct taatcgtcgc
     2761 gtttatcggt tttcggatgc tctttccgca acagaaagcg attgattcac cggaggcgaa
     2821 aagcaagtcg gaagagctgg aagatgaacc cagtgccaat attgcctttg tgccgctagc
     2881 aatgccaagt accgctggtc cgggaaccat agcgatgatt attagctcgg cgtccacagt
     2941 gcgtcagagt tcaaccttcg ctgattgggt actgatggtt gcgccgccgc tgatcttttt
     3001 cctggtggcg gttattttgt ggggaagcct acgcagctcc ggcgcaatta tgcggctggt
     3061 gggcaagggt ggcattgaag cgatctcccg tctgatggga ttcctgctgg tatgtatggg
     3121 tgtgcagttt attattaacg gcatcctgga aatcattaaa acgtaccatt aaaaaaggcc
     3181 cgaatgggcc tttcaactat tgcgtctgtt cttcgagtgt cactggccag cggcgaaata
     3241 taatgattga ccatattaac gcggcaaaag caggcaccgc gcccacataa ccaatcatcg
     3301 acattgacca gtgcaaactc acctgattac ctaccaacgc acccgccccg attccaatat
     3361 taaatatgcc ggagaatagc gccatcgcga cgtcggtagc atctggtgcc agcgccagca
     3421 ctttaacctg cataccaagc ccgatgatca tcatcgcgat cccccagaaa atactcagca
     3481 ccccgaggtg tatttcactg ttcgccgcag gtagcagcag tgccagacac accagcaaca
     3541 gcgcaatcgc cgtactcacc aacgcagacg catactgatt acccagttta ccgaaaatca
     3601 cgctgccaat aatgcccgca ccaccgagta ataacagtaa tgccgtggca aagttggcgc
     3661 tgaatcccgc aatgttttgc acaaaaggct cgatatagct gtatgccgtg taatgggcgg
     3721 tgacaaccac cacagttaac aaataaatgc tcatcaatgc cgggcggcgg aacaatagcg
     3781 ggaggctttt caatgaaccg gaatgctcac tgggcagtaa gggaagtaac ttaatcaggc
     3841 acaaaagggt gataagcgcc ccaataccaa tcgcgaagaa ggtcattcgc caaccgaaat
     3901 actgccccac aatgcgcccg agaggtaaac ctaagaccat cgccagtgct gtaccggtgg
     3961 caattaaact caaggcctgt gcccgcttcc cggccggagc catacggatc gccagagacg
     4021 ccgtaatcga ccagaaaatc gcatgtgcaa aagccacacc aatgcgactg atcaccagaa
     4081 cggtaaagct ccacgataaa aacgacagta cgtggctggc aataaacacc acaaacaggc
     4141 agatcagtaa tttgcgccgt tcaacctgac tggtcattaa cataaaaggc aatgacatta
     4201 gcgctactac ccatgcgtaa atggtcaaca tgatgccgac ctgagcggtt tgcatgtgaa
     4261 aactttgcgc aatgtcagag agcaggccaa cagggacaaa ttcggtggtg ttgaagatga
     4321 aggcggcgac tgccagcgta acgacccgta gccacgccac tttgcgggaa acagtgtttg
     4381 ttgtcataac agattatcgg gtgagttgcg aaaaagtgat gaaatgatct taaaacggtt
     4441 aatgatgtga atacaatgca tttgtgatct ggatcgcgtt ttccgtttct ctgacgctga
     4501 ttctgttccg cgtcagagag tcggtaacat cacggtttaa tgacgttcag ggaggtctgg
     4561 cgttttggcg gttgcgggta taggtggtct gacaggggag cgaggcctgt ttttcaccag
     4621 gtcttcgtga gtaacttttg gcggcgtttc attttttaca aacagtttga gggtcctgcg
     4681 cgccatatgc ttgataattt tatcatcact taatgatgct ggcatcaaca cgtcattcag
     4741 atcactttca ttgggattca taaattggtg atagtgcaat aacatattgg cgaaaaataa
     4801 aaaaaagtcc tgaaaatgag gagattcttg ttctgctaaa agggataaaa agcagatgca
     4861 gacttcgggt agtacatgtt tgttagaacc taatgaatcc atcaggcttt gcaataaatc
     4921 ctcttttaat tcgccgtctg ttatgtcgag aatggcggtg atcaagttag gatctttatt
     4981 gacgaaccat ctgtgaacct ca
//
LOCUS       DAJTNN010000230         4847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTNN010000230 DAJTNN010000000
VERSION     DAJTNN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.235"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1250)
                     /locus_tag="OO150_003859"
     CDS             complement(<1..1250)
                     /locus_tag="OO150_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGCS family amino acid carrier protein"
                     /protein_id="HCR5517829.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMA"
     gene            1529..2482
                     /gene="tal"
                     /locus_tag="OO150_003860"
     CDS             1529..2482
                     /gene="tal"
                     /locus_tag="OO150_003860"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5517830.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2597..3184
                     /gene="mog"
                     /locus_tag="OO150_003861"
     CDS             2597..3184
                     /gene="mog"
                     /locus_tag="OO150_003861"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5517831.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSVLTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(3219..3785)
                     /gene="satP"
                     /locus_tag="OO150_003862"
     CDS             complement(3219..3785)
                     /gene="satP"
                     /locus_tag="OO150_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5517832.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3934..4647)
                     /gene="msyB"
                     /locus_tag="OO150_003863"
     CDS             complement(3934..4647)
                     /gene="msyB"
                     /locus_tag="OO150_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5517833.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4673..>4847)
                     /locus_tag="OO150_003864"
     CDS             complement(4673..>4847)
                     /locus_tag="OO150_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5517834.1"
                     /translation="SASQSLNIPSEIKEGQTTDWININSDNDNKRCVSKITFSGHTVN
                     SSDMATLKIIGDD"
BASE COUNT         1275 a         1247 c         1226 g         1099 t
ORIGIN      
        1 gccattatga tatctgccag ttgccacatc agcggaaggc ttagcaaggt gccgccgatg
       61 accgttgcga aggtgcagat ccgcaaacac cagatcgctt taggggtgtt caggcgtaaa
      121 aagaagagat tgttttcggc ataaatgtag ttggcaacga tggagctgaa ggcaaacaga
      181 ataaccacga gggtaacaaa ctcagcaccc caggaaccca ttagcacccg catcgccttc
      241 tggataagct gaataccttc cagcggcatg taggttgtgt cgttacccgc cagtaatatc
      301 agcatggcgc ttgccgtaca gatgaccagg gtgtcgataa aaatgccaat catctggaca
      361 atcccttgcg ctgccggatg cggaggccag gacgccgctg ccgctgccgc gtttggcgtc
      421 gaacccattc ccgcctcatt ggaaaacata ctgcgctgaa aaccgttagt aatcgcctgg
      481 cttaaggtat atcccgccgc gccgcctgcc gcttcctgcc agccaaaagc actctcaaaa
      541 atagaccaaa tgacgtgggg aagttgcccg atatttatta cgcaaatcac catgctggtc
      601 agcacccaga ttatcgccat caacgggaca aacccctgca tgagccgggc gacgccatga
      661 agaccgcgag tgattgccag cagagcaaag acagcgagaa taatgcctgt caccagcggg
      721 ggaaaatcaa aagaaaaact cagggctcgg gcaacggcgt tcgcctgaac tccgctgaaa
      781 attatgccgt aggcgatgag caaaaagacg gcgaacagaa cgcccatcca acgcatcccc
      841 agcccgcgcg ccatatacca tgccggtccg ccacgaaact gcccattgac gtcacgttct
      901 ttataaagtt gtgcaaggga acattcggca aacgaggtcg ccatgccgat aaacgcggca
      961 acccacatcc aaaagacggc tctaggtcca ccggtggtaa tagccagcgc aacgccggcc
     1021 aggttgccgc tacccacgcg cgccgcaaga ctggtacaca atgactgaaa tgaggttaaa
     1081 ccgcctggct gtggatgaat gctattttta agacttttgc caaactggcg gatgtagcga
     1141 aactgcacaa atccggtgcg aaaagtgaac caacaacctg cgccgaagag caggtaaatc
     1201 attaccgatc cccaaaggac gctgttaata aaggagaaaa aatctggcat gcatatccct
     1261 cttattgccg gtcgcgatga ctttcctgtg taaacgttac caattgttta agaagtatat
     1321 acgctacgag gtacttgata acttctgcgt agcatacatg aggttttgta taaaaatggc
     1381 gggcgatatc aacgcagtgt cagaaatccg aaacagtctc gcctggcgat aaccgtcttg
     1441 tcggcggttg cgctgacgtt gcgtcgtgat atcatcaggg cagaccggtt acatccccct
     1501 aacaagctgt ttaaagagaa atactatcat gacggacaaa ttgacctccc ttcgtcagta
     1561 caccaccgta gtggccgaca ctggggacat cgcggcaatg aagctgtatc aaccgcagga
     1621 tgccacaacc aacccttctc tcattcttaa cgcagcgcag attccggaat accgtaagtt
     1681 gattgatgat gctgtcgcct gggcgaaaca gcagagcaac gatcgcgcgc agcagatcgt
     1741 ggacgcgacc gacaaactgg cagtaaatat tggtctggaa atcctgaaac tggttccggg
     1801 tcgtatctca actgaagttg atgcgcgtct ttcctatgac accgaagcgt caattgcgaa
     1861 agcaaaacgc ctgatcaaac tctacaacga tgctggtatt agcaacgatc gtattctgat
     1921 caaactggct tctacctggc agggtatccg tgctgcagaa cagctggaaa aagaaggcat
     1981 caactgtaac ctgaccctgc tgttctcctt cgctcaggct cgtgcttgtg cggaagcagg
     2041 cgtgttcctg atctcgccat ttgttggccg tattcttgac tggtacaaag cgaatactga
     2101 taagaaagag tacgctccgg cagaagatcc gggcgtggtt tctgtatctg aaatctacca
     2161 gtactacaaa gagcacggtt atgaaaccgt ggttatgggc gcaagcttcc gtaacatcgg
     2221 cgaaattctg gaactggcag gctgcgaccg tcttaccatc gcacctacac tgctgaaaga
     2281 gctggctgag agcgaagggg ctatcgaacg taaactgtct tacaccggcg aagtgaaagc
     2341 gcgtccggcg cgtatcactg agtccgagtt cctgtggcag cacaaccagg atccaatggc
     2401 agtagataaa ctggcggaag gtatccgtaa gtttgctatt gaccaggaaa aactggaaaa
     2461 aatgatcggc gatctgctgt aatcattctt agcgtgaccg ggaagtcggt cacgctacct
     2521 cttctgaagc ctgtctgtca ctcccttcgc agtgtatcat tctgtttaac gagactgttt
     2581 aaacggaaaa atcttgatga atactttacg tattggctta gtttccatct ctgatcgcgc
     2641 atccagcggc gtttatcagg ataaaggcat ccctgcgctg gaagaatggc tgacatcggt
     2701 gctaaccacg ccgtttgaac tggaaacccg cttaatcccc gatgagcagg cgatcatcga
     2761 gcaaacgttg tgtgagctag tggatgaaat gagttgccat ctggtgctca ccacgggcgg
     2821 aactggcccg gcgcgtcgtg acgtaacgcc cgatgcgacg ctggcagtag cggaccgcga
     2881 gatgcctggc tttggtgaac agatgcgcca gataagcctg cattttgtac caactgcgat
     2941 cctttcgcgt caggttggtg tgattcgcaa acaggcgctg atccttaact tacccggtca
     3001 gccgaagtct attaaagaga cgctggaagg tgtgaaggac gctgcgggta acgttgtggt
     3061 acacggtatt tttgccagcg taccgtactg cattcagttg ctggaagggc catacgttga
     3121 aacggcacca gaagtggttg ctgcattcag accgaagagt gcaagacgcg acgttagcga
     3181 ataaaaaaat ccccccgagc ggggggatct caaaacaatt agtgggattc accaatcggc
     3241 agaacggtgc gaccaaactg ctcgttcagt acttcaccca tcgccagata gattgcactg
     3301 gcaccacaga tcagaccaat ccagccggca aagtggatga ttgcggcgtt accggcaatg
     3361 ttaccgatcg ccagcagggc aaacagcaca gtcaggctaa agaaaacgaa ttgcagaacg
     3421 cgtgcgcctt tcagcgtgcc gaagaacata aacagcgtaa atacgcccca cagacccagg
     3481 tagacaccaa ggaactgtgc atttggcgca tcggtcagac ccagtttcgg catcagcaga
     3541 atcgcaacca gcgtcagcca gaaagaaccg taagaggtga atgcggttaa accgaaagtg
     3601 ttgccttttt tgtactccag cagaccagca aaaatttgcg cgatgccgct gtagaaaatg
     3661 cccatggcaa gaataatacc gtccagagcg aaataaccca cgttgtgcag gttaagcaga
     3721 atggtggtca tgccgaagcc catcaggccc agcggtgccg gattagccaa cttagtgttg
     3781 cccataattc ctcaaaaatc atcatcgaat gaatggtgaa ataatttccc tgaataactg
     3841 tagtgttttc agggcgcggc ataataatca gccagtgggg cagtgtctac gatcttttga
     3901 ggggaaaatg aaaattttcc ccggtttccg gtatcagacc tgagtggcgc taaccatccg
     3961 gcgcaggcag gcgatttgca gtacggctgg aatcgtcacg cgataggcgc tgccgctgac
     4021 cgctttaacc ccatttagtg ccgcacctac agggcctccc agccccgcgc cgcgcagcaa
     4081 accatgccca agtacgctca ttgctgcgtg ggtgcgtaaa atgcgggtca gttggctgga
     4141 aagcaaatgc gacacgcctt ttgccaataa tttgtctttc atcagcagcg gcagcagctc
     4201 ttccagctca ttcaccctcg catcgaacgc gtgcagaaac tcctgcttat gttcctcgtc
     4261 cattttcttc caggtattac gcagaaattg ttccagtaac tgttgctcaa tttcaaacgt
     4321 agacatctct ttgtcggctt tcagcttcaa tcgttttgaa acatcgagca aaatggcccg
     4381 atacaattta ccgtgtccgc gcagtttgtt ggcgatacta tcgccaccaa aatgctgtaa
     4441 ttctccagca atcagctgcc agttgcggcg atgttgctcg ggatgccctt ccatcgattt
     4501 aaacagttcg ttgcgcatca gtacgctgga gaggcgagtt ttgccttttt cattatgggt
     4561 gagcaatcgg gcgaaatttg ccaactgttc ctcactacaa tgctgaagaa aatccagatc
     4621 tgaatcattc aggtaattaa cattcatttt ttgtggcttc tatattctgg cgttagtcgt
     4681 cgccgataat tttcagcgtg gccatatccg atgagttcac cgtatgaccg gaaaaggtga
     4741 tttttgagac gcagcgttta ttgtcgttat cactgttaat gttgatccag tcagtggttt
     4801 gcccttcttt tatttctgaa ggaatattca gactctgact ggcgcta
//
LOCUS       DAJTNN010000231         4736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTNN010000231 DAJTNN010000000
VERSION     DAJTNN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.236"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            281..1060
                     /gene="deoC"
                     /locus_tag="OO150_003865"
     CDS             281..1060
                     /gene="deoC"
                     /locus_tag="OO150_003865"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5517835.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            1187..2509
                     /gene="deoA"
                     /locus_tag="OO150_003866"
     CDS             1187..2509
                     /gene="deoA"
                     /locus_tag="OO150_003866"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5517836.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDARALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            2561..3784
                     /gene="deoB"
                     /locus_tag="OO150_003867"
     CDS             2561..3784
                     /gene="deoB"
                     /locus_tag="OO150_003867"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5517837.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            3864..4583
                     /gene="deoD"
                     /locus_tag="OO150_003868"
     CDS             3864..4583
                     /gene="deoD"
                     /locus_tag="OO150_003868"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5517838.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1142 a         1208 c         1297 g         1089 t
ORIGIN      
        1 gcattttcat gagaagtggg catcttcttt tccttttatg ccgaaggtga tgcgccattg
       61 taagaagttt cgtgatgttc actttgatcc tgatgcgttt gccaccactg acgcattcat
      121 ttgaaagtga attatttgaa ccagatcgca ttacagtgat gcaaacttgt aagtagattt
      181 ccttaattgt gatgtatatc gaagtgtgtt gcggagtaga tgttagaata ctaacaaact
      241 cgcaaggtga attttattgg cgacaagcca ggagaatgac atgactgatc tgaaagcaag
      301 cagcctgcgt gcactgaaat tgatggacct gaccaccctg aatgacgacg acaccgacga
      361 gaaagtaatt gctctgtgtc atcagaccaa aactccggtc ggcaataccg ccgctatctg
      421 tatctatcct cgctttatcc cgattgctcg caaaacgctg aaagagcagg gcaccccgga
      481 aatccgaatc gctacggtaa ccaacttccc acacggtaac gatgacatcg aaatcgcgct
      541 ggcagaaacc cgtgcggcaa tcgcctacgg tgctgatgaa gttgacgttg tgttcccgta
      601 ccgcgcgctg atggcgggta acgagcaggt tggttttgac ctggtgaaag cctgtaaaga
      661 ggcttgcgcg gcagcgaatg tactgctgaa agtgatcatc gaaaccggcg aactgaaaga
      721 cgaagcgctg atccgtaaag cgtctgaaat ctccatcaaa gcgggtgctg acttcatcaa
      781 aacctctacc ggtaaagtgg cagtgaacgc gacgccggaa agcgcgcgca tcatgatgga
      841 agtgatccgt gatatgggcg tagaaaaaac cgttggtttc aaaccggcgg gcggcgtgcg
      901 tactgcggaa gatgcgcaga aatatctcgc catcgcagat gaactgttcg gtgctgactg
      961 ggcagatgcg cgtcactacc gctttggtgc ttccagcctg ctggcaagcc tgttgaaagc
     1021 gctgggccac ggtgacggta agagcgccag cagctactaa gcaagatgct ttacgcctga
     1081 tgcgctgcgc ttatcaggcc tacgagacgt atctacccgt aggccggata aggcgtagac
     1141 gcatccggca aaagccgcct catactcttt tcctcgggag gttaccttgt ttctcgcaca
     1201 agaaattatt cgtaaaaaac gtgatggtca tgcgctgagc gatgaagaaa ttcgtttctt
     1261 tatcaacggc attcgcgaca acactatctc cgaagggcag attgccgccc tcgcgatgac
     1321 cattttcttc cacgatatga caatgcctga gcgtgtctcg ctgaccatgg cgatgcgaga
     1381 ttcaggaacc gttctcgact ggaaaagcct gcatctgaat ggcccgattg ttgataaaca
     1441 ttccaccggc ggcgtcggcg atgtcacttc gctgatgttg gggccgatgg tcgcagcctg
     1501 cggcggctat attccgatga tctccggtcg cggcctcggt catactggcg gtacgctcga
     1561 caaactggaa tccatccctg gcttcgacat tttcccggat gacaaccgtt tccgcgaaat
     1621 tattaaagac gtcggcgtgg cgattatcgg tcagaccagc tcactggctc cggcggataa
     1681 acgtttctac gcgacccgtg atattaccgc aaccgtggac tccatcccgc tgatcactgc
     1741 ctcgatcctg gcgaagaaac tggcggaagg tctggatgcg ctggtgatgg acgtgaaagt
     1801 gggtagcggc gcgtttatgc cgacctacga actctctgaa gcccttgccg aagcgattgt
     1861 tggcgtggct aacggcgctg gcgtgcgcac caccgcgctg ctcaccgaca tgaatcaggt
     1921 actggcctcc agtgcaggta acgcggttga agttcgtgaa gcggtgcagt tcctgacggg
     1981 tgaatatcgt aacccgcgtc tgtttgatgt cacgatggcg ctgtgcgtgg agatgctgat
     2041 ctccggcaaa ctggcgaaag atgacgccga agcgcgcgcg aaattgcagg cggtgctgga
     2101 caacggtaaa gcggcagaag tctttggtcg tatggtagcg gcacaaaaag gcccgaccga
     2161 cttcgttgag aactacgcga agtatctgcc gacagcgatg ctgacgaaag cagtctatgc
     2221 tgataccgaa ggttttgtca gtgaaatgga tgcccgcgcg ctggggatgg cagtggttgc
     2281 aatgggcggc ggtcgtcgtc aggcatctga caccatcgat tacagcgtcg gctttactga
     2341 tatggcgcgt ctgggcgacc aagtagacgg tcagcgtccg ctggcggtta tccacgcgaa
     2401 agacgaaaac agctggcagg aagcggcgaa agcggtgaaa gcggcaatta aacttgccga
     2461 taaagcaccg gaaagcacac caactgtcta tcgccgtatc agcgaataac ggtatactga
     2521 tctgatcatt taaatttgaa gcactgagta cggagaacat atgaaacgtg catttattat
     2581 ggtgctggac tcattcggca tcggcgctac agaagatgca gaacgctttg gtgacgtcgg
     2641 ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa ggcgaagctg ataacggtcg
     2701 taaaggcccg ctcaatctgc caaatctgac ccgtctgggg ctggcgaaag cacacgaagg
     2761 ttctaccggt ttcattccgg cgggaatgga cggcaacgct gaagttatcg gcgcgtacgc
     2821 atgggcgcac gaaatgtcat ccggtaaaga taccccgtct ggtcactggg aaatcgccgg
     2881 tgtcccggtt ctgtttgagt ggggatactt ctccgatcac gaaaacagct tcccgcaaga
     2941 gctgctggat aaactggtcg aacgcgctaa tctgccgggt tacctcggta actgccactc
     3001 ttccggtacg gtcattctgg atcagctggg cgaagagcac atgaaaaccg gcaagccgat
     3061 tttctatacc tccgctgact ccgtgttcca gattgcctgc cacgaagaaa cctttggcct
     3121 ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag ctgaccaacg gcggctacaa
     3181 catcggtcgt gttatcgctc gtccgtttat cggtgacaaa gccggtaact tccagcgtac
     3241 cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg accgtgctgc agaaactggt
     3301 tgatgaaaaa cacggtcagg tggtttctgt cggtaaaatt gcagacatct acgccaactg
     3361 cggtatcacc aagaaagtga aagcaactgg cctggacgcg ctgtttgacg ccaccatcaa
     3421 agagatgaaa gaagcgggtg ataacaccat cgtcttcacc aacttcgttg acttcgactc
     3481 ttcctggggc caccgtcgcg acgtcgccgg ttatgctgcg ggtctggagc tgttcgaccg
     3541 ccgtctgccg gagctgatgt ctttgctgcg cgatgacgac atcctgatcc tcactgctga
     3601 ccacggttgt gatccgacct ggaccggtac tgaccacacg cgtgaacaca ttccggtact
     3661 ggtttacggc ccgaaagtaa aaccgggttc actgggtcac cgtgaaacct tcgcggatat
     3721 cggccagact ctggcaaaat attttggtac ttctgatatg gaatatggca aagccatgtt
     3781 ctgataatgg gtgcggcttg atgccctcac cccggccctc tcccacaggg agagggtgaa
     3841 aacatgttga taaggataaa acaatggcta ccccacacat taatgcagaa atgggcgatt
     3901 tcgctgacgt agttttgatg ccaggcgacc cgctgcgtgc gaagtatatt gctgaaactt
     3961 tccttgaaga tgcccgtgaa gtgaacaacg ttcgcggtat gctgggcttc accggtactt
     4021 acaaaggccg caaaatttcc gtaatgggtc acggtatggg tatcccgtcc tgctccatct
     4081 acaccaaaga actgatcacc gatttcggcg tgaagaaaat tatccgcgtg ggttcctgtg
     4141 gcgcagttct gccgcacgta aaactgcgcg acgtcgttat cggtatgggt gcctgcaccg
     4201 attccaaagt taaccgcatc cgttttaaag atcatgactt tgccgctatc gctgactttg
     4261 acatggtgcg taacgcggta gacgcggcta aagcactggg cattgatgct cgcgttggta
     4321 acctgttctc cgctgacctg ttctactctc cggacggcga aatgttcgac gtgatggaaa
     4381 aatacggcat cctcggcgtg gaaatggaag cggctggtat ctacggcgtc gctgcagaat
     4441 ttggcgcgaa agccctgacc atctgtaccg tgtctgacca catccgcact cacgagcaga
     4501 ccactgccgc tgagcgccag accaccttca acgacatgat caaaatcgca ctggaatccg
     4561 ttctgctggg cgataaagag taattgtgtt tcgctgaaag gcgattgtcg tgtgaagccg
     4621 gagtgggaga ctgctccggc tttttagtat ctattcatca atagcaactg atttatagat
     4681 ttactattat ttttcatatg gatagaaaat aaaatctatt caagtatcta ttcacg
//
LOCUS       DAJTNN010000232         4695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTNN010000232 DAJTNN010000000
VERSION     DAJTNN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.237"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..791)
                     /locus_tag="OO150_003869"
     CDS             complement(<1..791)
                     /locus_tag="OO150_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5517839.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWFNSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIPSAVMAACLWQLPGIRTHLVAFDTSVVDLTA"
     gene            complement(784..3063)
                     /locus_tag="OO150_003870"
     CDS             complement(784..3063)
                     /locus_tag="OO150_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517840.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQIALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQRNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLANAVTGCYLTPYSEKHLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASIADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DANFINALPDLRAVMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(3074..4162)
                     /locus_tag="OO150_003871"
     CDS             complement(3074..4162)
                     /locus_tag="OO150_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5517841.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1090 a         1245 c         1283 g         1077 t
ORIGIN      
        1 tctgccgtga gatcaacgac gcttgtgtca aacgccacca gatgggtacg aatgccgggt
       61 aactgccaca aacaggccgc catcaccgca gaggggatca ccgaatcgac catcgagccg
      121 ctttgatcaa ccagtaagac cagttgccat tgttcgcttt ggcgtttaat gcggctgtta
      181 aagcgggggg attcgatata caacttgccg tgttgcgggt gccagtgttg caggttggcg
      241 cgcagagtac ttttgaaatc aaagtttcgc gccagtggaa taaatgagcg gcgacggcga
      301 tcgcggacac cagaaaaagc ctgacgaact tcctttgcca gtcgcgccat aatttcttca
      361 acaacctggc gcacaatcct gcgggcggca gccagtactt cggggttcat cagatgtttg
      421 gtgtgcaaaa cggcgcgtag caggctttcg gaaggctgca tacgttccag cacgtcgaga
      481 tttgtcacca catcttcaat gccgtagcgc agtacggcat cgctttccag ccgctcaatc
      541 acctgttgcg gaaacagcgt gtggatactg ttgaaccact caggagtggt gagatttgag
      601 ccacctaatc caccagagcg ttcaccacgc tggagccgtt caggatcgcg cccatacagc
      661 cactccagcg cgtgatctat ctgccgggcg ttgtcatcca gcccacaaag cgtcgtttct
      721 gccgcttcgc caagaattaa tcgccagcgt tgtagctcac gggtggtcag aagatcgttc
      781 agttcagaca tggaaaactc cccagtgttg cagcgatgcc agtgcctgct gttcgagttg
      841 ttgatgatgt gcaatggctt gcggtggaca atgcaacggc atttgcagtg ccgaaacggg
      901 aagttgcgcc agttgataat gctcaagcac ctgatgcgcc agcgtcccgc gttctcgtgg
      961 cggtagccag gccatcaccg cgcgtaaatc gggcagggca ttgataaaat tggcatcact
     1021 cagttgattc aaatggctgc tgaaaccggc gataaatgca ggttgacagg ccagttgatg
     1081 acgggctaat gccagcaaac cgtgcagcgc ctcgccggat tgtgccgggg ataactgcgc
     1141 cagcatcgtc agcgccgctt cggcactggc gttgggatgt tccagacgca ttagtgcgcc
     1201 aagagccgcg ccgcgatcca gcgccggggc atgaatcgcc tgactgcgtc gctccagtaa
     1261 ggctaccgcc gcagaaagcg aaacgccgga taaattaacg ccgctatgta gatcgcgcag
     1321 aatatggcaa agcgcttgcc agctatgcag gtgagcgtga aactcctttt catccggtct
     1381 gccgttagat tcacacagcc acagcgtgcg atcgatagcc gcgcataacg tcgtttgtaa
     1441 tatctgcgcg ccttgcatac cgctaatttc atccagccgc cataaggcat atagcacttc
     1501 cagcgcctgg cccatttcgg caaattgatt ttcctgggcg attaataatg tgagttgctc
     1561 cagtaattgt tgactgaagg acgccagacc cgctaacgcc gcctggctaa gacatgtggt
     1621 gatactgccg atgccacccg cgtccagcat atcggcttct aatttatggc gtgcggcttc
     1681 ctgaagtgtt gcaccaaagc atgcggcctc aatcaatgcc gcatgctggc taagcgggcg
     1741 ggttaatttc cagtgttctt caccgttgcc tgcaagtgtc agtgtacttc cctgctggcg
     1801 tacaatccca gggatctcca gtattgccag ccgatgtaac acctgacttt gcgccagccc
     1861 atcgggggta aagcgattta aggtaagctc cgccggaaga gagattgcgg tacgttccag
     1921 ttcgcaggtg acatcttttg gcagaggcgg ttgtggcgtg gaaggggcaa gtttaccgaa
     1981 tccgtcaccc gctaatgtgt ctatcaacgt tagcagaatc ggatcggtat cgggatgaat
     2041 aacgccgcga tagctccacg gcaacggtgc attcagggct tctttaatca gcgagcctgc
     2101 tatggcatcc agccagtcag tgcgtaacgg tagtgtatga ccgcgcaact gtgccagtgc
     2161 cattgcatgc agatgtgcgg cagccatatc cgcgatcgaa gcaggcaagt tgtgctggcg
     2221 caaacgggtg agaaccgttt ttagcagttg ttcaccggcc tgctgtaagc cccactgcca
     2281 gcaccagttt tgccagaccg gggcaggcat accggaaagg tatcctgcca gcacatcaag
     2341 gtgcttttca ctgtaggggg tgagataaca acctgtaacg gcatttgcca gcgagggcaa
     2401 ttctggcttg ttaatttcct gcggacattc gcgccacatt tttgccagtg caggagcgtg
     2461 ccagccaccg cagacgacta acacgtcgcc attatttcgc tgcaccgccc atgcaatcca
     2521 gcgagccata aaggcttcgc gctgacgatt gagtgcatcg ccggggaaat cgccccgcag
     2581 ttgggcaaaa tagtgcgcca gcgcagaggg taatgcaatt tgctggcttt catcttcgaa
     2641 caagtgatcc cacagggtgt cgctgttatc catgcgggtg gcacgcagca gcaaggcctg
     2701 gctttcctct tgcgtatcag gcgaatcgtc cacttcttca ctctgcgccc agcaaggcaa
     2761 atcgatgaag taagtttgtg cctgaatacg acgtgcggct tgtagcgcct gccactccgg
     2821 cgaaaattca gcaaatggcg tccaggcacc acgcccgggg gctgcaccgt cctgatactg
     2881 gcaataactg taaatagcta ccggaagctg gtgggctaaa aacagttcgt ctacccggtc
     2941 attaaaatca gccgggcctt caatcaatac gtatcgtggc cgctggcttt cgattaacga
     3001 tttcaccagc cgggcgcagg ccggactatg atgccggatg ccgacaatta acggctcgct
     3061 catgcatctt tcctcacggc aggcggtggc gggcttgata ataagcctgc cagtgagctt
     3121 ctttatgtgt ggcgactcgt tgttcgaagt aacggcgcag acgagcgcga tcttcttcgt
     3181 tatctttgac gatggttccg gcaatgcagt ccaccaggtc ggctggctca cctgcgcgat
     3241 tcgctaaaaa ccaggcgcgg acgcctacgg cgtgggccac gttcacggct tcagcggtgg
     3301 acataatcgc tgttagtgta tccattgacg ttttcttttc gccgttggca cgcagatcgc
     3361 ggaaggtgcg gaccagtaac tccagtaccg cgtcaggcac tttatgcgga ataccgctat
     3421 gagccagcaa acgcgccgaa gcactggcga ccagttccag ctcctgggcg aaatccataa
     3481 tcggaaagac agtttcaaaa tcgaagcgac gctttagcgc agcactcatt tcattgacgc
     3541 cgcgatcgcg ggtattggca gtggcaataa tattaaagcc ttctcgcgca tagagctgac
     3601 tggcttcacc agtgagttcc ggcaccgtca tcaccctgtc agaaagcata cctaacagac
     3661 agtcttgcac ttccagcggt gtacgcgtta tctcttcaaa acggacgatt ttgccgtcgc
     3721 gcattccctg gtaaagcggg gcagggacca gcgcttccgt tgatggacca tgattgataa
     3781 gcagggcgta gttccagccg tacttgattt gatcttcggt agtagatgcg ccgccctgga
     3841 tggttaaccc ggcatcgccg ctaatagcgg ttgccagtaa ttcagacagg agagattttg
     3901 cggtgccagg ttcccccacc aacatcaaac cgcgtccggt ggcaagagtg actaacatac
     3961 gttcgacagc ggagggacta atcaccactt tacggctaat attttgcgcg ctatcgccaa
     4021 ggataaaagc acgggccgca ggcaaactta actgccaacc gggcgggcaa ggtgcgttat
     4081 cattttgttt taattttgcc agttcatcgg cgtataacac agcagcaggc ggacgctgca
     4141 gatgattatt ctgcggtgac atatattcat ccctgaaatg gattaatgaa ataaacgata
     4201 ttttacgcgc aataaatgtt tggcgcatta gttgtttgag cgaaaaatat ctggaaagca
     4261 gatcacagta aataaaaaaa cctgctggtg aggcaggttc ttttatttac tactttcagg
     4321 cctaacacca cagcctgtgc aacatcgtgt tccaacgaac gaattataac cacggctgga
     4381 ccccgttgca attaagataa tgagatttat aatccagacc gttgggacgt tgcgccggat
     4441 cgaatctatt tcaagtcctg gctaccggac gggtggtgct gcaggtggtg ccctgatccg
     4501 gagtgcaaca tcgtggtcca acatacgtaa accagtatcg acaggcagtg taagggtaag
     4561 gtgctggtgt tgcgctcctt aataaggaga gcggatggac acgctgacac aaaagttaac
     4621 cgtgctcatt gctgtactgg agctattagt ggctctgtta cggttgattg atttgttgaa
     4681 gtaacgggca acttg
//
LOCUS       DAJTNN010000233         4449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTNN010000233 DAJTNN010000000
VERSION     DAJTNN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.238"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1733
                     /gene="cysJ"
                     /locus_tag="OO150_003872"
     CDS             <1..1733
                     /gene="cysJ"
                     /locus_tag="OO150_003872"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5517842.1"
                     /translation="ATTDLTPTQLAWVSGYFWGVLNQQPAALAATPAPAAEMPGITII
                     SASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPP
                     EEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFFCQSGKDFDSKLAELGGERLLDR
                     VDADVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVASL
                     SVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGD
                     EPVTVEGKTLPLNEALQWHFELTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAAT
                     TPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENEVHVTVGVVRYDVEGR
                     ARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQ
                     RAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKDGVLTRIDLAWSRDQKEKVYVQDK
                     LREQGAELWRWINDGAHIYVCGDANRMAKDVEQALLEVIAEFGGMDIEAADEFLSELR
                     VERRYQRDVY"
     gene            1733..3445
                     /gene="cysI"
                     /locus_tag="OO150_003873"
     CDS             1733..3445
                     /gene="cysI"
                     /locus_tag="OO150_003873"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5517843.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPHHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3520..4254
                     /gene="cysH"
                     /locus_tag="OO150_003874"
     CDS             3520..4254
                     /gene="cysH"
                     /locus_tag="OO150_003874"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5517844.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
BASE COUNT         1097 a         1157 c         1278 g          917 t
ORIGIN      
        1 cggccacgac cgatttaacg cctacccagc ttgcctgggt ttctggctat ttctggggcg
       61 tactcaatca gcagcctgct gcgcttgcag cgacgccagc gccagccgca gaaatgccgg
      121 gtataactat tatctccgcc tcgcaaaccg gcaatgcgcg ccgggttgct gaagcattac
      181 gcgatgattt attagcagca aaactgaacg ttaagctggt gaacgcgggc gactataaat
      241 tcaaacaaat cgccagcgaa aaactgctca tcgtagtgac gtcaacgcaa ggggaagggg
      301 aaccgccgga agaagccgtc gcgctgcata agttcctgtt ctccaaaaaa gcgccaaagc
      361 tggaaaacac cgcgtttgcc gtgtttagcc tcggcgatag ctcttatgaa tttttctgcc
      421 agtccgggaa agatttcgac agcaagctgg cggaactggg tggtgaacgc ctgctcgacc
      481 gtgtcgatgc cgatgttgaa taccaggctg ctgccagcga gtggcgcgcc cgcgtggttg
      541 atgcgcttaa atcgcgtgcg cctgtcgcgg caccttcgca atccgtcgct actggcgcgg
      601 taaatgaaat ccacaccagc ccgtacagca aagacgcgcc gctggtggct agcctctctg
      661 ttaaccagaa aattaccggg cgtaactctg aaaaagacgt tcgccatatc gaaattgact
      721 taggtgactc gggcctgcgt taccagccgg gtgacgcgct gggcgtctgg tatcagaacg
      781 atccggcact ggtgaaagaa cttgtcgaac tgctgtggct gaaaggcgat gaacctgtca
      841 ccgtcgaggg caaaacgttg cctctgaacg aagcgctaca gtggcacttc gaactgaccg
      901 tcaacaccgc caacattgtt gagaattacg ccacgcttac ccgcagcgaa acactgctgc
      961 cgctggtggg cgataaagcg aagttacagc attacgccgc gacgacgccg attgtcgaca
     1021 tggtgcgttt ctctccggcg caactggatg ccgaagcgct gattaatctg ctgcgcccgc
     1081 tgaccccgcg cctgtattcc atcgcctcct cgcaggcgga agtcgagaac gaagtacacg
     1141 tcaccgttgg tgtggtgcgt tacgacgtgg aaggccgagc ccgtgccggt ggtgcctcca
     1201 gcttcctcgc ggatcgcgtg gaagaagagg gcgaagtccg cgtatttatc gaacataacg
     1261 ataactttcg cctgcccgct aacccggaaa ccccggtgat tatgattggc ccaggcaccg
     1321 gtattgcgcc gttccgcgcc tttatgcagc aacgcgccgc cgacgaagcg ccaggtaaaa
     1381 actggctgtt ctttggtaat ccgcacttta cggaagactt cctgtaccag gtggagtggc
     1441 agcgctacgt caaagatggc gtgctgacac gtatcgatct tgcctggtcg cgcgaccaaa
     1501 aagaaaaagt ttacgtacaa gacaaactgc gcgaacaggg cgcggagctg tggcgctgga
     1561 tcaatgatgg tgcccacatt tatgtctgcg gcgacgctaa tcgcatggcg aaagacgttg
     1621 agcaggcact tctggaagtg attgccgaat ttggtggcat ggacatcgaa gcggcggatg
     1681 aatttttaag tgagctgcgc gtagagcgcc gttatcagcg agatgtctac taatgagcga
     1741 aaaacatcca gggcctttag tggtcgaagg aaaactgaca gacgccgagc gcatgaagct
     1801 tgaaagcaac tacctgcgcg gcaccattgc ggaagattta aacgacggtc tgaccggcgg
     1861 ctttaagggc gacaacttcc tgctgatccg cttccacggc atgtatcagc aggatgaccg
     1921 cgacatccgc gccgaacgtg ctgaacagaa gctggagccg caccacgcga tgctgcttcg
     1981 ctgtcgtctg ccgggtgggg tgattaccac taaacagtgg caggcgatcg acaaatttgc
     2041 cggtgaaaac accatctatg gcagcattcg cctgaccaac cgccagacat tccagttcca
     2101 cggcattctg aaaaagaacg tcaaaccggt gcaccagatg ctgcactcgg tcggtcttga
     2161 tgcgctggcg acagctaacg acatgaaccg taacgtactc tgcacctcga acccttacga
     2221 gtcgcagctg cacgcggaag cgtacgagtg ggcgaagaag atttctgagc atctgttgcc
     2281 tcgtacccgc gcgtatgcgg agatctggct cgaccaggaa aaagtcgcca ctactgatga
     2341 agaaccgatc cttggtcaga cctacctgcc gcgtaagttc aaaaccacgg tagtgatccc
     2401 gccgcagaac gatatcgatc tgcatgccaa cgacatgaac ttcgtggcaa tcgccgaaaa
     2461 cggcaagctg gtaggcttca acctgctggt gggcggtggg ctttctatcg aacacggcaa
     2521 caagaaaacc tacgcccgca cggcgagcga gtttggctat ctgccgctgg agcatacgct
     2581 ggcggtggcc gaagctgtcg tgacaactca gcgtgactgg ggtaaccgaa ccgatcgtaa
     2641 aaatgccaaa accaaataca cgctggagcg cgtgggggtt gagacgttta aagcggaagt
     2701 ggagcgtcgc gcggggatca aatttgaacc gatccgtcca tatgagttca ccggacgagg
     2761 cgatcgtatt ggctgggtta agggcattga tgataactgg cacctgacgc tgtttatcga
     2821 aaatggtcgc atccttgatt atccggggcg tccgctgaaa accggcctgc tggagatcgc
     2881 gaagatccac aaaggcgatt tccgcattac ggcgaaccag aatctgatca tcgccggtgt
     2941 accggaaagc gagaaagcga agatcgagaa gatcgccaaa gagagcgggt taatgaatgc
     3001 cgtcacgccg cagcgtgaaa actcgatggc ctgcgtgtca ttcccgactt gcccgctggc
     3061 gatggcggaa gcagagcgtt tcctgccgtc ttttatcgac aacatcgata atttaatggc
     3121 gaaacatggt gtcagcgatg agcatatcgt gatgcgtgta acaggctgcc cgaacggttg
     3181 tggtcgcgcg atgctggcgg aagtgggcct ggtgggtaaa gcgccgggtc gctacaacct
     3241 gcatcttggc ggcaaccgca ttgggacacg tatcccacgg atgtataaag aaaacatcac
     3301 cgagccggaa atcctggcgt cgcttgatga actgataggg cgctgggcga aagagcgcga
     3361 agcgggtgaa ggcttcggcg actttacggt gcgtgcgggc atcattcgcc cggtgctcga
     3421 tccggcgcgc gatttgtggg attaaccatc agcccggtct tgtaggcctg ataagaacgc
     3481 gtgagcgtcg catcaggcaa ggcaaacagt gaggaatcta tgtccaaact cgatctaaac
     3541 gccctgaacg aactgccgaa ggtagatcgc attctggcgc tggcggaaac taacgccgaa
     3601 ctggaaaaac tggacgctga aggccgcgta gcctgggcgc tggataatct gcccggtgaa
     3661 tatgtacttt cttccagctt tggcattcag gcggcggtga gcctgcatct ggtgaatcaa
     3721 attcgcccgg atattccggt gatcctcacc gatacgggtt acttgttccc ggaaacctac
     3781 cgctttattg acgagttaac ggacaaactc aagctcaacc tgaaagtgta ccgtgctacc
     3841 gaaagcgcag cctggcagga agcacgctac ggaaaactgt gggagcaggg cgttgaaggc
     3901 attgaaaagt acaatgacat caacaaagtc gaaccgatga accgggctct gaaagaactt
     3961 aacgcgcaaa cctggtttgc tggcctgcgc cgtgaacaat ccggcagtcg cgccaattta
     4021 ccggtgctgg caattcagcg tggcgtattt aaagtgctgc cgattatcga ctgggataac
     4081 cgaactattt atcagtacct gcaaaaacat ggcctgaaat atcacccatt atgggatgaa
     4141 ggatatttat cggtcggtga tacccataca acccgtaaat gggaacctgg catgtcggaa
     4201 gaagaaacac gtttctttgg cttaaaaagg gaatgtggat tgcacgaagg gtaaatattg
     4261 ccggataaat taaacgcctc tgtcagaaat gatgggggcg tttttatttt gcgagcagta
     4321 ttcctgattt taaacgtgaa cgccgctgga agaattatca ggtatagaat ggcgtcgggt
     4381 gcttgaggct gtctggcgtt aagcattcgc gaggttccag atggacaaaa gccccaggcg
     4441 atatttcta
//
LOCUS       DAJTNN010000234         4429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTNN010000234 DAJTNN010000000
VERSION     DAJTNN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.239"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            254..616
                     /gene="queD"
                     /locus_tag="OO150_003875"
     CDS             254..616
                     /gene="queD"
                     /locus_tag="OO150_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5517845.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            694..1962
                     /locus_tag="OO150_003876"
     CDS             694..1962
                     /locus_tag="OO150_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5517846.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMGMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1953..2213
                     /locus_tag="OO150_003877"
     CDS             1953..2213
                     /locus_tag="OO150_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5517847.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFSITYRFG"
     gene            2230..2805
                     /locus_tag="OO150_003878"
     CDS             2230..2805
                     /locus_tag="OO150_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR5517848.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2953..3813)
                     /locus_tag="OO150_003879"
     CDS             complement(2953..3813)
                     /locus_tag="OO150_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5517849.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGIQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(3810..>4429)
                     /locus_tag="OO150_003880"
     CDS             complement(3810..>4429)
                     /locus_tag="OO150_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5517850.1"
                     /translation="RKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLR
                     FAPEFVARHIAEWQHQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWTCFTQVERFTLD
                     ALFITLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAA
                     EMPAMQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
BASE COUNT         1023 a         1181 c         1128 g         1097 t
ORIGIN      
        1 aagataaggt taacggggtg aacggtgtgg attaaagacg ggatagcgat aactaataac
       61 caaatcgact aacctgtttt agcaatagtc tttaacaaca aaatagatta accaacccaa
      121 tgaaaaacaa atgaatttag ccaatcatta agataaatca gcgattttgc gcaacaaagt
      181 cgttttagat aatgcgaaaa aacagccttt ccggtactct acggcggttt tatcgtcctg
      241 tagagaaatt atgatgtcca ccacgttatt taaagatttc accttcgaag ccgctcaccg
      301 cttaccacac gtcccggaag ggcataaatg tggtcgcctg cacgggcatt cctttatggt
      361 gcgactggaa attaccgggg aagtcgatcc gcatacgggc tggattatcg atttcgctga
      421 actaaaagcg gcgtttaagc caacttatga gcgcctcgat caccattatc tcaatgatat
      481 tccaggtctg gaaaacccaa ctagcgaagt tttagcaaaa tggatttggg atcaggttaa
      541 acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa acctgcaccg ctggttgtat
      601 ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt cccttaagta acgctatgtt
      661 agggtattgt gttcaggata tctggggcat gacatggaag acgactgcga cattattatt
      721 attggtgccg gtattgcagg caccgcttgc gcgttacgct gcgcgcgagc gggtttatcc
      781 gttttgttac tggaacgcgc tgaaatcccc ggcagcaaaa atctttccgg cggacggtta
      841 tatacccatg cactcgcgga actcctccct cagtttcatc tgaccgcgcc tcttgaacga
      901 cgcatcactc acgaaagcct ttccctgtta acgcccgatg gcgtaacgac gttttccagc
      961 ttacagcccg gcggtgaatc ctggagtgta ttacgtgcac ggttcgatcc gtggctggtt
     1021 gccgaagccg aaaaagaagg tgtcgaatgt atccccggag cgacggtgga tgcactgtat
     1081 gaagaaaacg gcagagtctg tggcgttatt tgtggtgacg atattctccg cgcccgttat
     1141 gtggtgctgg cagaaggtgc caacagcgtc ctggctgaac gtcacgggtt agtgactcgt
     1201 cctgctggcg aagcgatggc gttggggatc aaagaagtgc tgtcgctgga aacatccgct
     1261 attgaagaac gttttcatct ggagaataac gaaggcgcag cgttgctgtt cagcggcgga
     1321 atctgtgatg acttacccgg cggcgcattt ctttatacta atcaacaaac gctctcgtta
     1381 gggattgttt gcccgctctc ttcccttacg caaagtcgtg ttccggcaag cgagctgctg
     1441 actcgcttta aagcgcatcc ggcagtgcgc ccgcttatca aaaacacgga atcactggag
     1501 tatggtgcgc atctggtgcc agaaggtggc ttgcacagta tgccggtgca atacgccggt
     1561 aacggctggc agctggtggg cgatgcgttg cgcagttgcg tcaataccgg aatttccgtg
     1621 cgcggcatgg gtatggcgct gactggcgcg caggcggcac aaacgctgat aagcgcctgc
     1681 cagcaccgcg agccgcaaaa tctgtttgcg ctttatcatc acaacgtaga gcgcagcctg
     1741 ctatgggatg ttctacaacg ttatcagcat gttccggcgc ttttgcaacg ccctggctgg
     1801 tatcgggcgt ggcctgcgtt aatgcaggat atttcccgcg atttatggga tcagggtgat
     1861 aaacctgttc caccgctgcg ccagttattc tggcgtcatt tacgtcgtca tggcctgtgg
     1921 catctggcgg gcgatgttat caggagtctg cgatgtctgt agcccataat ctctggcgcg
     1981 ttgctgatgc gccgcacatt gttccggctg actccgttga gcgtcagatg gtacaatggt
     2041 tgattaacgc ctgtccggca ggtctttttt cgctcacacc ggaaggtgac ttacgcgttg
     2101 attatcacgg ctgcctggag tgtggcacct gccgtttgct gtgcgacgaa tccacactac
     2161 aacagtggcg ctatccgcct tccggattca gcatcaccta ccgctttgga taaataataa
     2221 ggataattta tgcccctctt acacctgctc cgccagaatc cggtgattgc tgccgttaaa
     2281 gataatgcca gcctgcaact ggcaatcgac tctgaatgcc agtttatttc cgtgttgtac
     2341 ggcaatatct gcaccatcag taatatcgtt aaaaagatta agaacgccgg gaaatatgct
     2401 tttattcatg ttgatttgct ggaaggcgcg tcaaataaag aagtcgtgat tcagtttttg
     2461 aaactggtga ccgaggcgga cggcattatc agtaccaaag cctcaatgtt gaaagcggca
     2521 agagcggaag gttttttctg tattcatcgc ctgtttattg ttgattcaat ttcgtttcac
     2581 aacattgata agcaagttgc gcaatcgaat ccggattgta ttgagatcct gccaggctgt
     2641 atgcccaaag tgctgggctg ggtgacagag aaaatccgcc aaccgctgat tgccggtggg
     2701 ctggtgtgcg atgaagaaga tgcgtgtaat gcgattaacg cgggtgtcgt ggcgctttcc
     2761 accacgaata ccggggtctg gacgttagcg aaaaaattac tttgacggga taatcgtaac
     2821 caattgaatt tggtttgatt tgtaggccgc acgccacatc cgacattcag cgcctgatgc
     2881 gacgcttgac gcgtcttatc aggcctacaa gtccccaggc cagaaccgta ggcaggatca
     2941 tccggcaaca cattattggc aatctgcgtg aatgtttgtc accagtgcct ccagcaccac
     3001 tttccagtca tcaaccacgc caacatctgc ctgtgaaaat accgcagcgc tggcgtcatg
     3061 attaatcgcc accacaaact tgctattgcg tacgcccgcc attaacgctg ctgcgccgga
     3121 agcgccaaca acaatacaca cctcaggtgc cagcaaatgt ccggaaatac caatcacttt
     3181 ttcagcatca acgccaccgt tcatcactcg cgcccgactg tagcccactt cggctcccag
     3241 cttttctgcc agcatagcga tttcctggtt atctgcctct ccgccctgcc ccaccaccag
     3301 cacccgtctg gcctcagcta atgggtcgcg agtaacattt ttcagatcct ctgtgctaac
     3361 cagccagtcg ggaagcgcac ccgggacaat gttcagttgt tgtattccag agggcaacgt
     3421 ggcgtttttc gctgctcccg cctgacgcgc cagagaaagg catagcgggc gtttttctgt
     3481 ttgcagcgtc gccgttagcg catttcccca gtgggatttg cgcacgctta cggttggaat
     3541 atctaacgaa gtcacctggc agatgcttgc accatgtaaa cgccaggcca gccgggtcga
     3601 cagttcatcg ccaaacgttc ccggcggaaa gagaacaacg tccgcaggcg ttcgctgcca
     3661 ctgttccacc aacgcatcca gcacctgttc cgccactaca ggctgaggtt caatctgcca
     3721 gtgcgccagg gtacaaccac tgaaatcctg cgccgccagc cagcttgcta ttgcggcatt
     3781 ctcctgattt attgtcacaa ttgcgatatt catggctgca tcctttggcg taaatagtcc
     3841 tgccatagct tttgagcttt ttctgctacc gtttgcccat caatcagcgt cgctccgcgt
     3901 cgttgctctg ctcttgccag ttgcagacac tgcatcgctg gcatttcggc agcaacagtt
     3961 ttacggataa tttctgcttt ccccgcagcc attcgctggc gcattcccgg tactggcaag
     4021 gccacttcac cgcactgacg cacggcaatc accgcaggca agcgaacccg gcaacaacgc
     4081 aacccatgtt cagtacgctg ttcgagggtg ataaacagcg cgtcgagagt gaaacgctcc
     4141 acctgggtga agcaggtcca gcccagcatt tccgccagca aaaatggcgt ttgcccattc
     4201 tgcccttcgc tgctttggct gccggtaatt atcagatcca gcggattctg atgctgccat
     4261 tcggcaatat ggcgagcgac aaattccggc gcaaagcgca gatctgccgc cgtctccagc
     4321 aatacagctt cctcaaaccc aagagccatg agatagcgca gccagtgcaa tgcccgttca
     4381 tcccccatgc tcaacgcggt taaagacatc ggcgtaccgt tttttctct
//
LOCUS       DAJTNN010000235         4403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTNN010000235 DAJTNN010000000
VERSION     DAJTNN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.240"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            227..599
                     /locus_tag="OO150_003881"
                     /pseudo
     CDS             227..599
                     /locus_tag="OO150_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1161..2108)
                     /gene="icsP"
                     /locus_tag="OO150_003882"
     CDS             complement(1161..2108)
                     /gene="icsP"
                     /locus_tag="OO150_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5517851.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGTHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3489..3641)
                     /locus_tag="OO150_003883"
                     /pseudo
     CDS             complement(3489..3641)
                     /locus_tag="OO150_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3697..4389
                     /gene="ospZ"
                     /locus_tag="OO150_003884"
     CDS             3697..4389
                     /gene="ospZ"
                     /locus_tag="OO150_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR5517852.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDCLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RFSQIKARMSVT"
BASE COUNT         1353 a          862 c          849 g         1339 t
ORIGIN      
        1 gcgatctggt tcaacaatat gcagatggta cacaacctct ttaaccagcc catccggatt
       61 attcagagcc cttccgcaat ccggaataaa agtgctgttt taccgtgaac cctctgaaaa
      121 tcagcgagca gtgttttaac cagccgacgt tcagagcgta ctgacagacg gtggacaatt
      181 tgaataaaca gttcaatcag accgtagatg ctctcccgtt ggcgacatgc tgggagcggt
      241 ggaacaccgc ttcggcaacg agcttccggc gtctccagtg aagtgactga cggataatgg
      301 ttcatgctac cgggctaatg aaacacgcca gttcacccgg atgttgggac tttgttgaac
      361 cgaagaacac ggcggtgcgg agtccggaga gtaacagaat agcaaagagc ttcgtgaaaa
      421 caataaagtg tgactacatc agtatcatgc ccaaacctga cgggttaacg gcagcaaaga
      481 accttgcaga ggcattcgag cattataacg aatggcatcc gcatagtgcg ctggattatc
      541 gctcgccacg ggaatatctg cggcagcggg ccaatgataa caggtgtctg gaaatatagg
      601 ggcaaagcca cccacgaacg atccagcata taatcaagaa attagatttc atttaacata
      661 agatagatta tgtgcaccaa gaggcatttc cttaatttct gaatttacag gctcttttgc
      721 tctctttgct atgctgtgga tttggaaaga tagagtgctg gctgattatt taggtatttt
      781 catcagcatg tccctgatag cactgttcca tcagcgcttt caggcgataa tagtagacct
      841 tgggtagcct ctgctcatgt gtaacttgat accccgagaa cagccacgat gatctaagcg
      901 gttcttattt ctccggtctc agggtcggta atgccaacag taagtccaca gtaatcaacg
      961 aagagttttt tgaaccgccc cggaaatcct ggagactaaa cgccctgaga aagaggtaaa
     1021 cagaatgact aaaaatacac gtttttcccc cgaagtccgt caacgggcag ttcgtatggt
     1081 tctggaaagt cagggcgaat atgactcaca atgggcggca atttttagcc atgaaaacgt
     1141 aatcaactcg gtgaaccaat tcaaaaaata tactttatac ctgcggaagt gagaaaacca
     1201 atatattcaa tacttgcact atttttaatg gtgccagata tattggtact ttgctcataa
     1261 agagatgtat cacctttttt attagaaatg taatttctgg agccttctat ataaaatttt
     1321 gcactggggg tgacatagta tccaatattc gcagcaacag agcagtaatt ttggtttttt
     1381 atttcatctt taaaaattgt ctcagtctga tagtgtttat ctgtatcaga tgaacgaacc
     1441 caaccactat atttcagttc tgctccaaac tcaaaattct catggcggta attagcagtt
     1501 aatccaatat aaggtatttt gaagagctgt ttgtaaccta ttgttctttc accactagga
     1561 tgtgtccctt ttttattcct gttccccccg ttttcactat aaatataact ccctcccatt
     1621 gcattaaaac tgtaacgact ctcttggtaa cctgctatta gtccaagtcg ataatccaaa
     1681 ttatttaata accaaccttt aagattcagc tcgaattcgt tagcatcacg gagatgcgta
     1741 ttgggatgcc agctctggtc ggtccatacc tgaggggtat tggaattgtt ccagtcttta
     1801 tcaaccatgc ttgctttctg gttacctaaa gtagtccaac cggaaactcc gaaagacact
     1861 tttggaatta gcttccaatc gatgccacct ctaacaatag tcgcattact gtatttccag
     1921 tccagttgac taatttttct ccccccttcc ttgggatgat aaacgcgttc ttttgttttg
     1981 ccactcagag atcctagact aatatccgta ctgatgttat ccggtatgaa aagtggatag
     2041 ttagtggtag catgtgtagt aaagatcgca ggtacacaaa gtgcaagtac aaggaatttt
     2101 aatttcatcg agaactctac ttttttggtt gaaatgtcca tgatcttctt actttataag
     2161 ataaacgtca atgccaagca ttattttaat taaatatttg tttatgttac caagaacata
     2221 tcttttatta taatatttat tgaacttttg taaaaataaa aaaatacctc attgtgcgaa
     2281 taaagtaacg ggggccatag atttctctac ctaccttttg gtgttcatac ctgaggctga
     2341 tagtttcagg attccgcgac aattctactc accttcttgt tctctcgcca atatgaaatc
     2401 aatcagaaaa gcgttccata agaacaacga taagacaata aattgtcatc acggctgcaa
     2461 tagccaacgt gcctttcagt accaacatga caacctcctg tatttgacgt aacgtgtgct
     2521 gataaatatg tatttgtatc tcaactgact caatacaaca cttaagtata atttttcaga
     2581 ttttgttttt cccgtgaaat attatggaaa tggcagaagc aatacattga attccgattc
     2641 gagtgaatac ggctagcggg cacctcactt tagcactgaa gccatcaact tttatttgac
     2701 ttatttcaat ggaaagcttt ctcactatca gtacaggtaa ttcccatcct tttaaagtcg
     2761 agagtcagac tgcgactaat acagttaaag tgaccaaact cagttatcct ctacacattt
     2821 tacctcatgt ttacttggac aattcattgg tgcttttata aaagaataaa tattctacta
     2881 atttaatgca tcacctaaaa tagtattttt tataatagaa ctgtatctat agtatattat
     2941 taaagcttaa ttatttatta tgctttaatg tggttgtccg attaaggact aatccttgaa
     3001 aacgccgtat agggcgggag ttcactctat tgcgataacg taaccgtaca atattgtgcg
     3061 tttcccaaat tgaccactac tgaactattc tgggatttaa atgcaataaa atggttggtt
     3121 gaaggtcgtg gtgatgatgt tagtcctttt gagaatattc cagtgccaga tcttacttat
     3181 aataaagagg cttggcagtt tggataagct attgtcctta ttccactaat acaaactttt
     3241 tgtaaagtat attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa
     3301 caattacacc ataaaaggaa gagggagccc caattaagtt atcaagcatg tttatgatac
     3361 ctgcagggta taaattttct ggctgcaagc agacacatat aaaacgtctt tattgatcct
     3421 acccacataa tatggacgca gtcgtaagtg aggctctgtg agcctaccca cgtaatatgg
     3481 acacaggcct aagcgaggtt ctggttttca aattgttccg gactgaggcc accacaccaa
     3541 cggtgccgtc gccagcgatt gtaatcgcac tcgatataat taaacactgt tgcccgcact
     3601 atttcccgat agataaagtg ttctccgtgg agacattcca cttaggaaca attatattgt
     3661 tcatggtaat gtttatattt gagtatagag attaaaatga ttagtcccat caagaatatt
     3721 aagaacgtat ttccaataaa tactgcaaat actgaatata tagtgagaaa tatttatcct
     3781 agagttgaac atggttactt taatgaatca ccaaacatat atgacaagaa gtatatctct
     3841 ggtataacca gaagtatggc acaattaaaa atagaggagt ttattaatga gaaatccaga
     3901 cgacttaatt atatgaagac aatgtattct ccatgtccgg aagattttca gcctatttcc
     3961 agagacgaag ccagtatacc ggaaggaagc tggttgacag ttatatctgg aaaacgtcct
     4021 atggggcaat tttctgtgga ttgtttgtac catcctgact tacatgcatt atgtgagctc
     4081 ccagagatta gttgtaagat cttccctaaa gaaaatagtg attttttgta tataattgtt
     4141 gtattcagaa atgattctcc ccaaggagaa ctacgggcaa atcggtttat agaactatat
     4201 gatataaaaa gagagattat gcaagtgtta agagatgagt cgccagagtt aaagtctatt
     4261 aaatctgaga ttattattgc tcgtgaaatg ggagaacttt ttagttatgc atcagaggaa
     4321 atagatagct acataaaaca aatgaatgac aggttttctc aaatcaaggc tcgaatgtct
     4381 gttacttaat gtatattaat tgt
//
LOCUS       DAJTNN010000236         4321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTNN010000236 DAJTNN010000000
VERSION     DAJTNN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.241"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..977)
                     /locus_tag="OO150_003885"
     CDS             complement(<1..977)
                     /locus_tag="OO150_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR5517853.1"
                     /translation="MIRKSATGVIVALAVIWGGGIWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGNFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVA"
     gene            complement(1132..1758)
                     /gene="dsbA"
                     /locus_tag="OO150_003886"
     CDS             complement(1132..1758)
                     /gene="dsbA"
                     /locus_tag="OO150_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5517854.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(1775..2761)
                     /gene="srkA"
                     /locus_tag="OO150_003887"
     CDS             complement(1775..2761)
                     /gene="srkA"
                     /locus_tag="OO150_003887"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5517855.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(2838..3107)
                     /locus_tag="OO150_003888"
     CDS             complement(2838..3107)
                     /locus_tag="OO150_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5517856.1"
                     /translation="MKCKRLNEVIEFLQPAWKKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            3177..3761
                     /gene="mobA"
                     /locus_tag="OO150_003889"
     CDS             3177..3761
                     /gene="mobA"
                     /locus_tag="OO150_003889"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5517857.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            3743..4270
                     /gene="mobB"
                     /locus_tag="OO150_003890"
     CDS             3743..4270
                     /gene="mobB"
                     /locus_tag="OO150_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5517858.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALYARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGARHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         1171 a         1020 c          965 g         1165 t
ORIGIN      
        1 gcaacttcat cgtttgctaa ctcagggtgt gcagcaagct gcttctgcat cgcaatgtta
       61 tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat caatgaccac
      121 tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta ggtcagattg
      181 acattgatat aatctttgtc tttaacgcga tccagcctgg tttttgcaac gaaagcatcg
      241 atttgggcaa cgtcttcccc ctgattgata atattaacct gattaaattt cgactcgctt
      301 tcaccaaccg gaaatttctt ttcttctaat cgtgtcagag aatcaatggt aaaacttttt
      361 gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt gccttcaata
      421 tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc accaaaagaa
      481 aaatttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc accaaacgcg
      541 aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag cggttgtgtc
      601 agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac attaccatgc
      661 attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatgc aactttctgg
      721 cctggcttga tattgagatc gggtgcaccg ttatcaaatg aaatttgcat ttgaaaatgg
      781 gaattaaaaa agcctttgtc aaagttttta taacttaacg tcatatcgta ggcatgttca
      841 cctttctttt tagcatcgtt aaaatcttta ataaattttt cgacaccagg ctgaatttgc
      901 gtacctgtgt accatatgcc accaccccag attacggcta acgcaacaat aacacctgta
      961 gctgacttac gtatcatata aatattccct gataaaacag caaaactttg aatatccact
     1021 tatgctgaaa taatgtccag cggcaggatg tattatcaga atgtatttat atttattaaa
     1081 taataaaaaa agcccgtgaa tattcacggg ctttatgtaa tttacattga attatttttt
     1141 ctcggacaga tatttcactg tatcagcata ctgctgaaca aaaacatcca tattgctggt
     1201 atccataccc tgcggattca gctgatattt accgttaaca aacatcgccg gaacaccgcg
     1261 caattgcacg tcagctgcag ctttttcctg ctgagctacc agagatttca ccacgaagct
     1321 gttccacgcc gcgtcgtact cttcaccttt aatacctgcg ttgataaata catcgcggat
     1381 atcagaagca gaacgaatgg tctgggtttt ctgtacgcct tcaaacagcg gaacagtcac
     1441 tttgtcttcc acgcccagcg ccatcgccac agcccatgcc tgagtcagat ctttgcccag
     1501 gtcaccaccc atgaagttga cgtggtattt agtcatcttc acgccttccg gcagtttttt
     1561 cttcacatta tcagaaatat gcagaacttc ttcaaactga tagcagtgcg gacagaagaa
     1621 agagaaaaac tccagcactt gcggcgcgcc agctaccggt ttttccaggg tagtgtactg
     1681 tttaccatct tcatactgcg ccgccgatgc gctaaacgct aaaactaaac cagccagcgc
     1741 cagccaaatc tttttcatga tctactctct ccgattaata cataggtgtt aattgtaaag
     1801 ggggttcttg tagaactttt gcctgttcta taaaagtcgc cgtttgtcgc agccagtaat
     1861 cttccccggt taaccacggg aaatttttcg ggaacgcggg atcagcccaa cgccgcatta
     1921 gccaggcaag ataataaacc aaacgcatgg cgcgtaaagg ttcaatcagt ccgatttcag
     1981 cggtgtcgaa ctcgctaaat tcttcataag cttcaataat agtttccagt tgcatccgct
     2041 gctcggcttt atcgccattg agcaacatcc acaaatcctg aatagctgga ccattacgtg
     2101 catcatccag atcaacaaac attggaccat cgcgccagag aatattcccg gcgtggcagt
     2161 ctccatgtaa gcgcagaacg gtggaatctt cccgccagtg cgctgtaacg gcggcgatca
     2221 gctcatctgt cgctttcagg aatgccgctt tcaacccgga aggtatcagt gtagcgtcct
     2281 caaacagctt gcgtggctca atgagatatt cattcagacc gatggtcggg cgatggataa
     2341 aaagctgttt gcgccccgtc tggtgcatac gccctaaata acgcccaacc gcttccatct
     2401 gatcgatatt atcagcttcg aactggcgac caccgacgct tggaaaaaca gcgaaataaa
     2461 atccctgatg attcaataaa gtctgaccgt taaaggccac aggtgctgcg accggaactt
     2521 catcatttac caactgcaac gcaaattgat gttcttcgag gatttgatcg gctgtccaac
     2581 gttcagggcg ataaaatttg acgacaatac gtcgacgatc ttcgtcctga aattgataga
     2641 cacggttttc atagctgtta agcggggtaa gaccggaatc cacccggatc ccttgctcaa
     2701 atagagcatc catgatggta tccgggtgta gtgtctggaa agtaaaagcg ctgttgttca
     2761 tcccatcatc cggaaaacat tacgaatgat gaaggatatc atttcacgat gattttggtg
     2821 gcgccgctta caagctttta ctctttaatt acgccgcgag cgcgtaacag cgccgttttg
     2881 aagtcttctt cataatcttt ctgcaaaccc gggatcaccg catctttcgc ggaatcacgc
     2941 attttcaggt gatagatcag aatgtcatcc gtcaaatccg ccagttcgcc gtcaaaacct
     3001 gactctttcg ccagtttctg caaaaattgc aacaggttaa ggtctggctc ttttttccag
     3061 gctggctgga ggaattcaat aacttcattc agacgtttac atttcatagt gatgctcctt
     3121 actcttgaga cagacacgtt agcagggtca atcccacaat aaaagaggcg atatcggtga
     3181 atctgatgac gacgataaca ggcgttgtgc tggcaggcgg taaagccaga cgaatgggtg
     3241 gcgtagataa aggattgctt gaattaaacg gcaaaccatt atggcaacat gtcgctgacg
     3301 cgcttatgac gcagctctct cacgtcgtgg ttaatgccaa tcgtcatcag gaaatctatc
     3361 aggcaagcgg tctgaaagtg attgaagatt cactggcgga ttacccaggc cctctggcag
     3421 gaatgctttc agtaatgcag caggaagctg gtgagtggtt tttgttttgc ccgtgcgata
     3481 cgccttacat tccccctgat ttagcagccc ggcttaatca tcagcgcaaa gatgcgcctg
     3541 tcgtgtgggt ccatgacggt gaacgcgatc acccgactat tgctctggta aaccgcgcta
     3601 ttgagccttt attactggaa tatctgcaag caggagaacg ccgggtaatg gcatttatgc
     3661 gtctggctgg cggtcatgcg gttgatttca gcgatcataa agatgcattt gttaacgtga
     3721 atacgccaga ggagcttgcc cgatggcagg aaaaacgatg ataccgttac tcgcctttgc
     3781 cgcgtggagt ggtaccggaa aaactacgct tttgaaaaaa ctgatcccgg cattatacgc
     3841 cagagggatc cgtccagggc tgattaagca tacgcaccat gatatggatg ttgataagcc
     3901 aggcaaagat agctatgagc tgcgcaaggc tggcgcggca caaaccatcg ttgccagcca
     3961 gcaacgatgg gccttgatga cggaaacacc agacgaagaa gagctggatc tacagtttct
     4021 cgcaagtcga atggatacct caaagctgga tttgattctg gtcgaagggt ttaagcatga
     4081 agagatcgca aagattgtgc tgtttcgcga tggagccaga catcgacctg aagaattagt
     4141 gatagacagg catgttattg ctgtagccag tgatgtgccg cttaatcttg atgtcgcatt
     4201 actggatatt aatgatgttg aggggctggc tgattttgtg gtggagtgga tgcaaaagca
     4261 gaacggataa gcaagagtag gctggatcag acgcagccag tgtcgcatcc gacctttttc
     4321 t
//
LOCUS       DAJTNN010000237         4296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTNN010000237 DAJTNN010000000
VERSION     DAJTNN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.242"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            49..2211
                     /gene="ybiO"
                     /locus_tag="OO150_003891"
     CDS             49..2211
                     /gene="ybiO"
                     /locus_tag="OO150_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR5517859.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2271..3197)
                     /gene="rlmF"
                     /locus_tag="OO150_003892"
     CDS             complement(2271..3197)
                     /gene="rlmF"
                     /locus_tag="OO150_003892"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5517860.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3473..3732
                     /locus_tag="OO150_003893"
                     /pseudo
     CDS             3473..3732
                     /locus_tag="OO150_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            3997..>4296
                     /locus_tag="OO150_003894"
     CDS             3997..>4296
                     /locus_tag="OO150_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5517861.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT          998 a         1133 c         1076 g         1089 t
ORIGIN      
        1 ccagcatcta tacttatctt tttgctctgt tttctcactg gaggagtcat gcggtggatc
       61 ctgttcatcc tcttctgcct gctgggcgca cctgcccacg cggtatccat acccggcgtt
      121 acaaccacaa cgacaacgga ctcaacgact gaaccggccc cggaaccgga tatcgaacaa
      181 aaaaaagcgg cctatggcgc actggcggat gtgctggata acgacacctc gcgtaaagaa
      241 ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg cggaaccggt cccgaagatc
      301 gtgccgccga cgctggttga agagcaaacc gtgctgcaaa aggtcaccga agtcagccgc
      361 cattatggtg aagccctttc cgcccgcttc gggcaacttt atcgcaatat taccggctcc
      421 ccgcataagc cgtttaatcc acaaacattc agcaatgcgc tgacccattt ttcaatgtta
      481 gcggtattag tgtttggttt ttactggctg attcgcctgt gcgcactgcc gctgtatcgc
      541 aaaatgggcc tgtgggcgcg gcaaaaaaat cgtgagcgca gtaactggtt gcagcttccg
      601 gcgatgatta tcggggcgtt tattatcgac ctactgttac tggcactgac attatttgtc
      661 ggccaggtat taagcgacaa cctgaatgca ggcagtcgca ccatcgcttt ccaacaaagt
      721 ttgtttctca acgcctttgc cctgattgaa tttttcaaag ctgtactacg cctgattttt
      781 tgcccaaacg tggctgaact gcgcccgttc acgattcatg acgagaccgc ccgttactgg
      841 agtcgtcgcc tgagctggtt aagcagtttg ataggttatg gcctgattgt ggccgtgccg
      901 attatctcta atcaggtgaa tgtacagata ggtgcgctgg cgaacgtcat cattatgctg
      961 tgcatgaccg tctgggcgtt gtacctgatc tttcgcaata aaaaagagat tacccaacat
     1021 ttgctcaact ttgcggagca ttcgctggcc tttttcagcc tgtttatccg cgcctttgcg
     1081 ctggtgtggc actggctggc aagcgcctat tttatcgtgc tgtttttctt ttcgttgttc
     1141 gatccgggca acagcctgaa atttatgatg ggtgcaacgg tgcgcagcct ggcgattatt
     1201 ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc tggcgaaaac catcaccctc
     1261 tcgccacata ctcagcgtaa ctatccggag ttgcaaaaac ggctgaatgg ctggctttcg
     1321 gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg cggtaatgtt gctgttgagc
     1381 gcatggggat tgttcgattt ctggaactgg ctgcaaaacg gcgcggggca gaaaaccgta
     1441 gatatcctga tccgtatcgc acttattctt tttttctcgg cggttggctg gacggtgctc
     1501 gccagtttga tcgaaaaccg gctggcttcg gatattcatg gccgcccgct acccagcgcc
     1561 cgtacgcgta ccctgctgac gctgtttcgt aacgcgctgg cggtgattat cagtaccatc
     1621 accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg cgccattgct ggcaggtgcc
     1681 ggggcattag gtctggctat ctcgtttggt tcgcaaacgc tggtgaaaga tatcattacc
     1741 ggggtgttta ttcagtttga aaacggcatg aacactggag atttggtgac tatcgggccg
     1801 ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg gcgtgcgcca ggataccggg
     1861 gcgtatcaca tcattccgtg gtcttcgata accacctttg ctaacttcgt ccgcggtatt
     1921 ggttcggtag tggcaaacta cgatgttgat cgccatgaag atgctgataa agccaatcag
     1981 gcactgaaag atgcggtagc ggaattgatg gaaaacgaag aaattcgcgg gctgattatt
     2041 ggtgaaccga attttgccgg gattgtcggc ttaagcaata ccgcgtttac actgcgtgtt
     2101 tcgttcacca cgctgccact caaacagtgg acggtacgct ttgccctcga cagccaggtg
     2161 aaaaaacatt tcgacctggt gggcgttcgc gcgccagtgc agacttatta ggtgctgcct
     2221 actccgggcg cgaccccggc tgaaccgtta ccgccggggg agccaacgct ttaacgctgg
     2281 cgattgacaa aacggcggcg ctgctcgtcg ttcataaagg tccaggcaat aaagcgactc
     2341 tgcttttgcc cctgggccat ctcttttttt accaccttca ccgcgcccac gtcggtcaga
     2401 gcacgataca acggcggtaa gttttcacca cgagatacca gcgaggtaaa ccacatcacc
     2461 tgcttcgcga agcctttgct ctcttcaatc atctttttga taaaggcgac ttcaccacct
     2521 tcacaccaca actcctgttg ctgaccacca aagttcagtg catcgtcttt gttcagcccc
     2581 aggttacggc gtttacgctc actacctgcc cgcgctgcag cggcggaatc gtggaatggc
     2641 gggttacaca aggtcgcgtc gtattgctcg tttttatgga tgatgccgtt aaaaattgcc
     2701 ccactctctt tttgccgacg cagacgaatg gcgcggttaa gccccggatt agcactgata
     2761 atcgcctgcg cactgcttaa cgcctggctg ctggtttcac taccggtaaa acgccagcca
     2821 tattcatgta cgccaatcag cggataaata cagttcgcac caacgccgat atccagaatg
     2881 ctggcattcg ctggaattgt tccgcttgct tcagccagta aatcggcaag gtgatgaata
     2941 taatccgccc ggccgggtac tggtgggcag agaaaaccgt cggggatatc ccagttcgct
     3001 acggcgtaaa aatgggccag caacgcctta ttgagcgcct tcaccgccag cggattggca
     3061 aagtctacgc tttgctcccc ggcgggtgta agcgtgagga attgcctgag ttcaggattg
     3121 acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac ggttgcgcgg atgcaacccc
     3181 ggtttctggg cggacatagc attctccttt aagcagcggc gtaagatacc cgcataacgc
     3241 gacagggtaa atatctggct gcgtttatca ttccccaaaa ccttaacatg gctatggttt
     3301 atggcaattc atcataaaaa tcagcattat gcatgtcatt gatttgtctt attttgtaaa
     3361 aatatatcgt tcaaaaaata agcacatctc tgtcaatttt ttgcgtcatt tgcatcgcgc
     3421 aggcgaccta cacttatcag gcactacccg ttgtatttat gaggatgtca ttatgaaaaa
     3481 gtgcctcaca ctactgattg ccaccgtcct gagcagaatc tctctcacgg cttatgccgc
     3541 gcaaccgatg agtaacctgg acagcggtca gctacggccc gcggtaccgt ttcggcaact
     3601 ggcgcatcaa acctaagcga tctggaggat aaactggcag aaaaagcgcg cgaacaaggc
     3661 gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc agatgttcgg tactgcaacc
     3721 atctacaaat aaccccacca acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt
     3781 ttgtaatcaa aaaacatcaa atatgaactc aatgtaaata aatgtatttc tttttcgcgc
     3841 aatgggtgat agaaaatcgc gccaagtgat aatgcttatc aaaattatta tcacttgcac
     3901 gaacactatc acgggattaa cagtggcatc gcatccgcag agatgctttc tcgtgacagt
     3961 gaaaatttca acatataaga aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct
     4021 gccagacaat ttcattcgct cacgttcttt gccggtcttt gtattggcat cacgcctgtg
     4081 gctcaggcac tcgccgccga agggcaaact aacgcggatg acacgctggt tgtcgaagca
     4141 tcaacgcctt cgctttatgc gccacaacaa tctgccgatc cgaaattctc gcgtccggta
     4201 gcggatacta cccgcacgat gacggtgatt tctgaacaag tgattaaaga tcagggcgca
     4261 accaacctta ccgacgcgct caaaaacgtc cccggc
//
LOCUS       DAJTNN010000238         4295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTNN010000238 DAJTNN010000000
VERSION     DAJTNN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.243"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..752)
                     /locus_tag="OO150_003895"
     CDS             complement(<1..752)
                     /locus_tag="OO150_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR5517862.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLQQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLL"
     gene            complement(883..2247)
                     /gene="sdaA"
                     /locus_tag="OO150_003896"
     CDS             complement(883..2247)
                     /gene="sdaA"
                     /locus_tag="OO150_003896"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5517863.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(2431..3009)
                     /locus_tag="OO150_003897"
     CDS             complement(2431..3009)
                     /locus_tag="OO150_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5517864.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVIGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(3013..>4295)
                     /gene="pabB"
                     /locus_tag="OO150_003898"
     CDS             complement(3013..>4295)
                     /gene="pabB"
                     /locus_tag="OO150_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5517865.1"
                     /translation="LPWAMLLHSGYADHPYSRFDIVVAEPICTLTTFGKETVVSESEK
                     RTTTTDDPLQVLQQVLDRADIRPTHNEDLPFQGGALGLFGYDLGRRFESLPEIAEQDI
                     VLPDMAVGIYDWALIVDHQRHTVSLLSHNDVNARRAWLESQQFSPQEDFTLTSDWQSN
                     MTREQYGEKFRQVQEYLHSGDCYQVNLAQRFHATYSGDEWQAFLQLNQANRAPFSAFL
                     RLEQGAILSLSPERFILCDNSEIQTRPIKGTLPRLPDPQEDSKQAEKLANSAKDRAEN
                     LMIVDLMRNDIGRVAVAGSVKVPELFVVEPFPAVHHLVSTITARLPEQLHASDLLRAA
                     FPGGSITGAPKVRAMEIIDELEPQRRNAWCGSIGYLSFCGNMDTSITIRTLTAINGQI
                     YCSAGGGIVADSQEEAEYQETFDKVTKILRQLEK"
BASE COUNT         1065 a         1115 c         1110 g         1005 t
ORIGIN      
        1 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
       61 ggaccattaa cgttaatggt aaaaggaaag cgttgcgaag aaacctgata gttttcattg
      121 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
      181 accgtcaggc tggcgctact gatttgctgc agttgtggct caaggagcat tgccgtcagt
      241 aagtcgatgt taatcatctc catgacccca gcattgctac tgcccgctgc aggcgtccat
      301 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
      361 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
      421 ctggagcaat agagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
      481 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
      541 aagacttcgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
      601 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
      661 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacgtcga
      721 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
      781 aaacgcctga cgcaacagtg gatgtgtagt ggggaatatt acagcagacg agaaagcggg
      841 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
      901 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
      961 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     1021 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     1081 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     1141 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     1201 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     1261 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     1321 cgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     1381 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     1441 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     1501 ggaaaccagc atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     1561 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     1621 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     1681 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     1741 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     1801 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     1861 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     1921 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttctac
     1981 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     2041 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     2101 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     2161 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     2221 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     2281 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     2341 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     2401 agtgttttgt agatgtaaag ataagtatag tcagggtttc acaccaattt gcagcgccag
     2461 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     2521 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     2581 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     2641 cagatcgggc gggataatgc cgatcactgg ggttacctga tagccagtta cgctatcgac
     2701 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     2761 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     2821 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     2881 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta gggtttcccg
     2941 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     3001 gtattccacg tcttacttct ccagttggcg taatatctta gtaactttat caaaagtttc
     3061 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     3121 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     3181 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     3241 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     3301 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     3361 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     3421 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     3481 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     3541 tggtagcgtg cctttaatcg ggcgggtctg gatttcacta ttatcacaaa gaataaaccg
     3601 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     3661 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     3721 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     3781 gcgaaatttt tcgccgtact gctcgcgggt catattggat tgccagtcgg aagtgagcgt
     3841 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     3901 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
     3961 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
     4021 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
     4081 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
     4141 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt taccgaaagt
     4201 ggttaaagtg caaatcggct cggcgaccac aatatcaaag cggctatacg gatgatcggc
     4261 atagccggag tgtaaaagca tcgcccacgg caggt
//
LOCUS       DAJTNN010000239         4264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTNN010000239 DAJTNN010000000
VERSION     DAJTNN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.244"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1089
                     /gene="nadB"
                     /locus_tag="OO150_003899"
     CDS             <1..1089
                     /gene="nadB"
                     /locus_tag="OO150_003899"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR5517866.1"
                     /translation="VVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSG
                     DGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPD
                     FDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLGID
                     LTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECL
                     VYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIV
                     RTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRG
                     LHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1074..1811)
                     /gene="trmN"
                     /locus_tag="OO150_003900"
     CDS             complement(1074..1811)
                     /gene="trmN"
                     /locus_tag="OO150_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5517867.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1943..3277
                     /gene="srmB"
                     /locus_tag="OO150_003901"
     CDS             1943..3277
                     /gene="srmB"
                     /locus_tag="OO150_003901"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5517868.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3310..4191)
                     /locus_tag="OO150_003902"
     CDS             complement(3310..4191)
                     /locus_tag="OO150_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5517869.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCIEECFLPG"
BASE COUNT         1079 a         1165 c         1082 g          938 t
ORIGIN      
        1 gttgtaggcg cgtgggtatg gaaccgaaat aaagaaacgg tggaaacctg ccacgcaaaa
       61 gcagtggtac tggcaaccgg cggtgcgtcg aaggtgtatc agtacaccac caatccggat
      121 atttcttctg gcgatggcat tgctatggcg tggcgcgcgg gctgtcgggt tgccaatctc
      181 gaatttaatc agttccaccc taccgcactg tatcacccac aggcacgcaa tttcctgtta
      241 acggaagcac tgcgcggcga aggcgcttat ctcaagcgcc cggatggcac gcgttttatg
      301 cccgattttg atgagcgcgg cgaactggcc ccgcgcgata ttgtcgcccg cgccattgac
      361 catgaaatga aacgcctcgg cgcagattgt atgttcctcg acatcagcca taagcccgcc
      421 gattttattc gccagcattt cccgatgatt tatgaaaaat tgctcgggct ggggattgat
      481 ctcacacaag aaccggtacc gattgtgcct gctgcacact atacctgcgg tggtgtaatg
      541 gttgatgatc atgggcgtac ggacgtcgag ggcttgtatg ccattggcga ggtgagttat
      601 accggcttac acggcgctaa ccgcatggcc tcgaattcat tgctggagtg tctggtctat
      661 ggctggtcgg cggcagaaga tatcaccaga cgtatgcctg atgcccacgg cgtcagtacg
      721 ttaccaccgt gggatgaaag ccgcgttgag aaccctgacg aacgggtagt aattcagcat
      781 aactggcacg agctacggct gtttatgtgg gattacgtcg gcattgtgcg cacaacaaaa
      841 cgtcttgagc gcgccctgcg gcgtatcaat atgttgcaac aggaaataga cgaatattac
      901 gcccacttcc gcgtgtcgaa taatttgctg gagctgcgta atctggtgca ggttgcggaa
      961 ttgattgttc gctgtgccat gatgcgcaag gagagtcgtg gcctgcattt cacactggat
     1021 tatccggaac tgctcaccca ttccggtccg tcgatccttt cccccggcaa tcattacata
     1081 aacagataaa aagcctgggt cagcgccgta tacgcttcgg aatagttctg gtctggccca
     1141 cgaatgacta agcgatcgct aaagcattct cccgcctgcg gggagaatgc cagcagcacc
     1201 cgatgcggca gtcgcgcttc gttttccgcc acatccgtcc gcaaacgtaa atgccagccc
     1261 atgcttaatg ccagctccgt aaaaccatta ccaatctgct ctggcagcac tacgcagaaa
     1321 aatccctctt cggtaatgca ctccgccgca caggtcagca gcgatgggtg atcaagcgta
     1381 gtggtatagc gagcctgttc ccgttgaggg atcgcgcact ctactccctg ctgatagtaa
     1441 ggtgggttac tgatgattaa atcgaagcgt actgtctgct gtgtgagcca ctgctgaata
     1501 tccgccgtat ggacgttaat ccgctctgcc cacggggact ggttgatatt ttcctgcgcc
     1561 tgcgcggcag cttcactttc cagctcaaca gcatcaatta tcacgctgtc actggttcgc
     1621 tgcgccagca tcagtgccag caacccgcta cccgcgccga tatcaaggca acgtttcacc
     1681 ccagccaccg gtgcccatgc gcccagcaaa atgccatccg ttcccacttt catcgcacag
     1741 cgatcgtgag caacaaaaaa ctgtttaaaa gtaaatccat tacgacgaag cacggatgta
     1801 gactgtgaca tgaaaataaa accttgcagg aaaaacggcg atagcaccgg gtgagaacaa
     1861 tacccgagaa gcgatatcca tacaaacaga tgaagattgc agccgtaacg tctataatca
     1921 gcgccccaca cagaggtaga acatgactgt aacgactttt tccgaacttg aactcgacga
     1981 aagcctgctg gaagccctcc aggataaagg tttcactcgc ccgaccgcca ttcaggctgc
     2041 cgccattccg cctgcgctcg atggccgtga tgtactcggt tctgcgccga caggcaccgg
     2101 taaaacggcg gcgtatctgc tgccagcgtt gcagcacctg ctcgatttcc cgcgtaagaa
     2161 atccggtccg ccgcgtattt tgatcctcac cccaactcgc gagctggcga tgcaggtggc
     2221 cgatcatgcc cgcgaactgg cgaaacatac gcatctggat atcgccacca ttaccggcgg
     2281 cgtagcctat atgaaccacg cggaagtgtt cagcgaaaat caggacatcg tggtcgccac
     2341 gaccggacgt ctgctgcaat acataaaaga agagaacttc gattgccgcg cggttgaaac
     2401 gctgatcctc gacgaagcag accgtatgct ggatatgggc ttcgctcagg atatcgaaca
     2461 tattgctggc gaaacgcgct ggcgtaaaca gaccctgctc ttttcggcaa cgctggaagg
     2521 cgatgcgatt caggactttg ccgagcgtct gctggaagat ccggtggagg tttctgccaa
     2581 tccgtccacc cgtgagcgca aaaaaattca tcagtggtat taccgcgccg atgacctgga
     2641 acataaaacc gcattgctgg tgcatctgtt aaaacagccg gaagcgaccc gctcaattgt
     2701 gtttgtgcgt aagcgtgaac gtgtgcatga gctggcaaac tggctgcgcg aagcgggcat
     2761 caacaactgc tatctcgaag gtgagatggt acagggcaag cgtaatgaag cgattaaacg
     2821 cctgaccgaa ggccgcgtaa acgtactggt agcaaccgat gttgccgcgc gcggtatcga
     2881 cattccagac gtcagccacg tctttaactt cgatatgccg cgcagtggtg atacttattt
     2941 gcaccgtatc ggacgtaccg cgcgcgccgg tcgtaaaggc accgcgattt cgctggtgga
     3001 agcccatgac catctgctgc ttggcaaagt tggccgttat attgaagagc cgataaaagc
     3061 tcgcgtcatt gaggaactac gcccgaaaac gcgtacgcca agcgagaagc agaccggcaa
     3121 gccatcgaag aaagtactgg caaaacgcgc cgagaagaaa aaagctaaag agaaagagaa
     3181 gccgcgggtg aaaaaacgcc atcgcgacac caaaaatatt ggtaagcgcc gtaaaccgag
     3241 cggaacgggc gtgccaccgc aaacgacaga agagtaatct taattccggg tttaagcccg
     3301 gcattatttt tatcccggca aaaaacactc ttcaatacac tgaataaaag tgtgcatcgc
     3361 cgggctaacc gcttttccag catggtgtgc gcacatagcc gtaatggtct gcgactgttc
     3421 gccaaaaggc aattcaatta attctccgct ctcaagctct ttttccaccg caaagcgggg
     3481 caagtaactg acaccgatat tcgccgcgac acaacgtttg atactttcaa tgcttaacag
     3541 ctcaatggtg ttttccaccg tgatccgccg ctgacgcagc gtgctctcaa atatctgccg
     3601 gaagacacat tgcggttcgt tgataataaa gctacaggcg ttatgtcttc ccggctcagt
     3661 aaaatcgaca tctgcaattt gcggtgaagc caccagcacc agcgattgtt cacccaactc
     3721 tcgtcggttc agggcatcat cattccctac gcgataaaag acgccgacat cggcctcatc
     3781 attcagtagc gcatcacgga tcacgtaaca gttcagcgac tgcaacgata aacgcacttt
     3841 tggtgcgcgt tgccgaaaac gctgcaacac ctgcggcatt cggtaagaga gcaacgtttc
     3901 gcccgaaaca acgcgtagtt ccccgtccgg atctgacccc tttttggcag cctcacgaag
     3961 cgtatccatc actcgggtaa gttcataaat gtgcggcagc aacttttttc cttcgcgggt
     4021 aaggcacatc cgtcggccaa ttttctcaaa taactggact gaaaactcct gctcaagctg
     4081 ctgaatatga aaagtcaccg tcgattgtgt acagcacaat ttttgcgaag ctcgcaaaaa
     4141 ggaaccctct tccaccacgg ttttaagcgt aataaaacgg cgcagatcca taaccccgaa
     4201 cctatcgaaa atatcgaatc tagaatataa aaacattcat ttttttaaat gttctgtgtc
     4261 gggt
//
LOCUS       DAJTNN010000240         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTNN010000240 DAJTNN010000000
VERSION     DAJTNN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.245"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO150_003903"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO150_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5517870.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO150_003904"
     tRNA            complement(324..399)
                     /locus_tag="OO150_003904"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO150_003905"
     tRNA            complement(406..480)
                     /locus_tag="OO150_003905"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO150_003906"
     tRNA            complement(597..681)
                     /locus_tag="OO150_003906"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO150_003907"
     tRNA            complement(690..765)
                     /locus_tag="OO150_003907"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO150_003908"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO150_003908"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5517871.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO150_003909"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO150_003909"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5517872.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO150_003910"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO150_003910"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5517873.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNVVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1079 a         1041 c          932 g         1131 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctaggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc acattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tag
//
LOCUS       DAJTNN010000241         4129 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTNN010000241 DAJTNN010000000
VERSION     DAJTNN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.246"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(37..594)
                     /gene="ymfL"
                     /locus_tag="OO150_003911"
     CDS             complement(37..594)
                     /gene="ymfL"
                     /locus_tag="OO150_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HCR5517874.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDIEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(638..838)
                     /locus_tag="OO150_003912"
     CDS             complement(638..838)
                     /locus_tag="OO150_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5517875.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTQGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            929..1603
                     /locus_tag="OO150_003913"
     CDS             929..1603
                     /locus_tag="OO150_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5517876.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPIGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALHPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3133..3669
                     /locus_tag="OO150_003914"
     CDS             3133..3669
                     /locus_tag="OO150_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HCR5517877.1"
                     /translation="MSFIQTLTGKKFNYINSTIDDIYIEDIAGALSNICRFAGHLPDF
                     YSVAQHSVLCSQLVAPEFAFEALMHDATEAYCQDIPAQLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPLLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            3660..4022
                     /locus_tag="OO150_003915"
     CDS             3660..4022
                     /locus_tag="OO150_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517878.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     LENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGDWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
BASE COUNT         1256 a          899 c          877 g         1097 t
ORIGIN      
        1 tcagttgcga gcgacggtag tgtgttgtta aactgttcat gcgttagttt ctccacaacc
       61 agaagcaatc gacgccacga cgcccggagc tgcacactcg cgggcgtcat tactttctga
      121 aatgcaaaaa attttgtaga caagtgctgc atgctcctgc agcttcgaaa ttgagagata
      181 cagctcgtcg ttaattgctg tcttctcatg cggttccact acaccgtctt cgattgctga
      241 acgaatctgt tttgaataac tgccgatctg ttcaatgact tccagcagac gctggttaat
      301 atcggcgttg tccacatcct cgatgtcagg aagagacaca aagacgccat ttgcagactg
      361 cgccacagca tcagcaatga agtgagtgcc accagcacgc tgtaaaacca ttgcccatcc
      421 cagcgggaaa atctgatcgc catctgcacg aaggcggttg aataaagcgt tttctgttac
      481 atcgagccag tcagccgctt cagcgtaacc acccggcaac tttgcgatag tttttctgac
      541 agctttcacg taccactcag gctgtttttc tattttccag tgatgcttac ccacgattag
      601 cctcatcgtt ctgtggttaa aaattgaaag tgttctgcta atctttcgga tagatatccg
      661 gtcttaagtc agatttcgta attgcacctg acgtgcattg ctcaagtttt ttagccagca
      721 caaaactggc ttttttatag ccattgaaaa ccagccgtaa gtagccttgt gttgagccaa
      781 cttttccggc caactcaccc tgctgttctt tggttaaaga gtcccaatac gctttcatac
      841 aatatgtacc tccagtgtac acatcacatg attgaaatga accttcaaga tacttgtacc
      901 ttagcggtac aagggtttta atttcgttat gaaaacaatc catgacatcc ggcggtctaa
      961 cgccagaaaa ctgagagatg gtgttggcgg gaattcttcc tttgccacta tgattgatcg
     1021 cgagccaacc cagaccagca ggtttatggg agatggtgct actaaaaata tcggtgacag
     1081 catggcacga cacattgaaa aatgtttcga cctgcctatc ggatggctcg atcaagaaca
     1141 ccagacaacg aacatcacaa aaaaacctga tgtttcaatc actaacaaac aaataacgtt
     1201 agtccctgtc atatcatggg tacaggccgg agcatggaaa gaagttggct attctgaggt
     1261 tgatttgagc acagcagaaa cttatccctg ccctgtaccc tgtggcgaaa tgacttatat
     1321 cttgcgggtg attggtgatt caatgattga tgagtaccgt ccgggagaca tgatttttgt
     1381 tgatcctgaa gtccctgctt gccacggtga cgacgttatt gcgttgatgc acgatacagg
     1441 cgaaaccacc ttcaagcgat tgatagaaga tggaacacag cgttatctca aagcattaca
     1501 cccaaactgg cctgagccct acattaagat taacggtaat tgctctataa ttggcactgt
     1561 gattttctcg ggaaaaccaa gaagatacaa aataaaggcc taatcaatat ttatgaacct
     1621 gcttcggcag gtttttttat acttgacaat gtaccccatg gacacacaat gtacccccat
     1681 ggacacacaa tgtaccaaaa cgaaacaaca aacaggcagg atgcccacga agtagccgcc
     1741 tggggcatat gaagtccaag atgattcgtt agcaacaaaa cgcccatagg acgcttcgct
     1801 ctttaacaat ttgaatattt tcagcgtcaa tgatctgttt agctccctac aagtaaatgt
     1861 agcgtagcac catgataaat cctgctagtg tttagttaaa aatcgcgcga gagcaagaac
     1921 gacgtgaggg caggcaatac tgatagtgtt attgctatta gtttatcaaa aatatttaga
     1981 cgacccagat gaatattttt aatacatact ccccctaatt taacaggagt aaatgttgat
     2041 gaataacgag acatctatac tcaacaattg tatgatatga acaattatat aacataatgt
     2101 aggaaaaata aaaatggatt ggcatgacct tatatcaaag gtaattatcg gacttgttac
     2161 aggctcaatc gcagcttact taactgcgcg atatgcactc ggccgttatt ataaagaaaa
     2221 atggtgggac aaaagaatag atttgtatac gtaattagtt gactgtgttt atcttctaaa
     2281 aaacacagct gactattggt ataaacatga gttacatcac gcttcacaat tcccaaccta
     2341 ctttagttac aaatcaaaag aagaaaatga aatccttagc aaatctttct ctaatgagct
     2401 tgaaaaactc agtagaatag gtgagctatc ttcattattc ttaacagaag agtgtgggga
     2461 taaaatccgt tcattcattg aaaatgaaga aaatctattt atacaatggg tgtttgatag
     2521 tatagagttg atagatgccc acaagtcaag acttgataaa ttaaaagtat tactagacga
     2581 tattatctct gaagcaaaga gagagcttaa ggtcaataaa aaacataaca gcctaaagaa
     2641 tataaagtgg tccataacta tgccagacca aaaaaatgat gagccaatta attatcacga
     2701 caatcattaa aacactttag aacccgctgg gcgggttcta ttcaaatata ctttgcacat
     2761 cacaaataaa actataacgt ccttcttctt ttcctaatat cttatatcta cttatttata
     2821 agcggcttac agatcttcaa cagataaacc taatgaaggc gacaatgttc tgatgaatag
     2881 ccaccttttt actcaactga aagcgcgttc tgtcccttgt cattaagtgc cagttcgtta
     2941 agtccaaaac ctccggaacg cgctttcaat tgagtgggga aaataactgg cgatggcagc
     3001 cgcccgttgc actaagtgcc tctggatggg gcatttactg aaacgaaacc aaaactttta
     3061 atcgccttat ggcgagggat tcgtgcaacc aaaaatcgtg cggtgcagcg caccaattaa
     3121 ggagaactaa cgatgagttt tatccagact cttaccggta agaagttcaa ttatatcaac
     3181 tccaccattg acgatattta tatcgaggat atcgccggtg cgctttcaaa tatctgccgc
     3241 ttcgcgggac acttgccgga tttttacagc gtggcgcagc attccgttct gtgcagtcaa
     3301 cttgtggcgc cagagttcgc ctttgaagcg ttaatgcatg atgcaacaga agcgtattgt
     3361 caggacatcc ccgcgcaact gaaacgcctt cttcctgact ataaacggat ggaagaaaaa
     3421 atagacgccg taatccgtga gaaatacggg ttacccccgg ttatgagcac gcccgtgaaa
     3481 tatgccgatc tcatcatgct ggcaaccgaa cgccgcgatc ttgggcttga tgatggctct
     3541 ttctggcctc tactagaagg tatcccagca acagagatgt tcaaagttat tccactgtcg
     3601 ccaggccatg cctacgggat gtttatggaa cgttttaacg aattatcgga gttacgcaaa
     3661 tgcgcatgaa tgttttcgaa atggaagggt ttcttcgcgg gaaatgtgta ccgcgagatc
     3721 tgaaagtgaa tgaaacggat gctgaatacc tggtgcgtaa attcgatgcg cttgaagcta
     3781 aatgtgcggc actggaaaac aaagtaatac cagtgtcaac tgaactgcca ccagcaaatg
     3841 aaagtgtttt gttattcgat gctaacggag aaggctggct aattggctgg cgttctctct
     3901 ggtacacctg gggacaaaaa gaaaccggag actggcagtg gacatttcag gtcggggacc
     3961 ttgaaaacgt caatatcact cactgggcag taatgccaaa agcaccggag gctggagcat
     4021 aatgaccact tttaccgaca aagaactgat taaagaaatt aaagagcgta tcagcagcct
     4081 tgacgtgcga gacgatattg agcgccgtgc ttatgaaatc gcactccta
//
LOCUS       DAJTNN010000242         4106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTNN010000242 DAJTNN010000000
VERSION     DAJTNN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.247"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(42..212)
                     /locus_tag="OO150_003916"
                     /pseudo
     CDS             complement(42..212)
                     /locus_tag="OO150_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
     gene            522..2294
                     /gene="aspS"
                     /locus_tag="OO150_003917"
     CDS             522..2294
                     /gene="aspS"
                     /locus_tag="OO150_003917"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5517879.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2412..2864
                     /gene="nudB"
                     /locus_tag="OO150_003918"
     CDS             2412..2864
                     /gene="nudB"
                     /locus_tag="OO150_003918"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5517880.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDSQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2893..3633
                     /locus_tag="OO150_003919"
     CDS             2893..3633
                     /locus_tag="OO150_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5517881.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3668..>4106
                     /gene="ruvC"
                     /locus_tag="OO150_003920"
     CDS             3668..>4106
                     /gene="ruvC"
                     /locus_tag="OO150_003920"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5517882.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAI"
BASE COUNT         1013 a         1012 c         1156 g          925 t
ORIGIN      
        1 tcaaccggct gttttaatgc ttcggcgtaa tcggcagatc agccaactcg caccagaaac
       61 acgggctgac cgctggcgcg aaatttcgcc gccagcttcc cggcgcgatt aactacctca
      121 tcggcagtat gtggaccacc ggcaaaaggt aagatgcctt cttgtaaatc gatcaccacc
      181 aacgcagtgg ttttagcatt aagttcaagc atgctgactc ctgaaaaaac aaaatcgtgc
      241 ctcacacctt aatgcgatta gccgatggcg gaacgacaaa ttttgttaat ttttatgagt
      301 tcgggcaaca cctttatagc aaactggcgt agagtctgat ttcaggcccg atataaagtg
      361 gtaacgaggc gttccagcgc gggctaggct cttacggtgc ggcagaattt ccagtataat
      421 agccgccttt tttcattcag ttgtgacata cagctaacgc tgcgacttgg tcacctgcgc
      481 agcagggggc atccgccggc agctaagtta agggatatct catgcgtaca gaatattgtg
      541 gacagctccg tttgtcccac gtggggcagc aggtgactct gtgtggttgg gtcaaccgtc
      601 gtcgtgatct tggtagcctg atcttcatcg atatgcgcga ccgcgaaggt atcgtgcagg
      661 tatttttcga tccggatcgt gcggacgcgt taaagctggc ctctgaactg cgtaatgagt
      721 tctgcattca ggtcacgggc accgtacgtg cgcgtgacga aaaaaatatt aaccgcgata
      781 tggcgaccgg cgaaatcgaa gtgctggcgt cctcgctgac tatcatcaac cgcgcagatg
      841 ttctgccgct tgactctaac cacgtcaaca ccgaagaagc gcgtctgaaa taccgctacc
      901 tcgacctgcg tcgtccggaa atggctcagc gcctgaaaac ccgcgctaaa atcaccagcc
      961 tggtgcgccg ttttatggat gaccacggct tcctcgacat cgaaactccg atgctgacca
     1021 aagccacgcc ggaaggcgcg cgtgactacc tggtgccttc tcgtgtgcac aaaggtaaat
     1081 tctacgcact gccgcaatcc ccacagttgt tcaaacagct gctgatgatg tccggttttg
     1141 accgttacta tcagatcgtt aaatgcttcc gtgacgaaga cctgcgcgct gatcgtcagc
     1201 ctgagtttac tcagatcgat gtggaaactt ctttcatgac cgcgccgcaa gtgcgtgaag
     1261 tgatggaagc gctggtgcgt catttgtggc tggaagtgaa gggcgtggat ctgggcgatt
     1321 tcccggtaat gacctttgcg gaagcagaac gccgttatgg ttctgataaa ccggatctgc
     1381 gtaacccgat ggaactgacc gacgttgccg atctgctgaa atctgttgag tttgcagtat
     1441 ttgcggggcc ggcgaacgat ccgaaaggcc gtgttgccgc tctacgtgtt ccgggtggcg
     1501 catcgctgac ccgtaagcag atcgacgaat acggtaactt cgttaaaatc tacggcgcga
     1561 aaggtctggc ttacatcaaa gttaacgaac gcgcgaaagg tctggaaggt atcaacagcc
     1621 cggtagcgaa gttccttaat gcagaaatca tcgaagccat cctggatcgt actgccgcgc
     1681 aagatggcga tatgattttc ttcggtgccg acaacaagaa aattgttgcc gacgcgatgg
     1741 gcgcactgcg cctgaaagtg ggtaaagacc ttggtctgac cgacgaaagc aaatgggcac
     1801 cgctgtgggt tattgacttc ccgatgtttg aagacgacgg tgaaggcggc ctgacggcaa
     1861 tgcaccatcc gttcacctca ccgaaagaca tgactgctgc agaactgaaa gctgcaccgg
     1921 aaaatgcggt ggcgaacgct tacgatatgg tcatcaatgg ttacgaagtg ggcggtggtt
     1981 cagtacgtat ccataatggt gatatgcagc agacggtgtt tggtattctg ggtatcaacg
     2041 aagaggaaca gcgcgagaaa ttcggcttcc tgctcgacgc cctgaaatac ggtactccgc
     2101 cgcacgcagg tctggcattc ggtcttgacc gtctgaccat gctgctgact ggcaccgaca
     2161 atatccgtga cgttatcgcc ttcccgaaaa ccacggcggc agcgtgtctg atgactgaag
     2221 caccgagctt tgctaacccg actgcactgg ctgagctgag cattcaggtt gtgaagaagg
     2281 cagagaataa ctgatatgac tcaaatacac gaaatcattc gcgttgcatc aaggcggcaa
     2341 ttgagtgaac tcccatgagc atagataact atgtgaatgg gatgagcgaa ggcagtcaac
     2401 gaagaggcag cgtgaaggat aaagtgtata agcgtcccgt ttcgatctta gtggtcatct
     2461 atgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg tgacgatccc gatttctggc
     2521 agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc atgcgcgaag
     2581 taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt gacagtcagc
     2641 gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg ggcgtgacgc
     2701 gtaatacgga atcatggttc tgtcttgcgc ttccgcacga gcggcagatc gttttcactg
     2761 aacatctggc ttacaagtgg cttgatgcgc ctgctgcggc ggcgctcact aagtcctgga
     2821 gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc ctgaacaggc agggctatct
     2881 ggagatattt ttatggcagg tcatagtaaa tgggccaaca ccagacatcg taaagctgcg
     2941 caggatgcta agcgcggtaa aatcttcact aaaatcattc gtgagctggt aaccgcggct
     3001 aagctgggcg gtggcgatcc ggacgctaac ccgcgtctgc gtgcggcggt ggataaagca
     3061 ctgtctaaca acatgacccg tgacacactg aaccgcgcaa ttgcacgtgg tgtgggcggt
     3121 gatgatgatg caaacatgga aaccatcatc tacgaaggtt acggtcctgg cggcacggca
     3181 atcatgattg aatgtctgtc tgacaaccgc aaccgtaccg ttgctgaagt gcgtcatgca
     3241 ttcagcaaat gtggcggtaa cctcggtaca gatggttccg tagcctatct gttcagcaaa
     3301 aaaggcgtga tctccttcga gaaaggcgat gaagacacca tcatggaagc agctctggaa
     3361 gcaggtgctg aagacgttgt gacctatgat gacggcgcga ttgatgtcta cactgcatgg
     3421 gaagaaatgg gtaaagtgcg cgacgctctg gaagcggcag gtctgaaagc agacagcgcg
     3481 gaagtttcca tgatcccgtc caccaaagct gatatggatg cagaaaccgc accgaaactg
     3541 atgcgtctga tcgatatgct ggaagattgc gacgacgtgc aggaagttta ccataacggt
     3601 gaaatctctg atgagattgc agcgactctc tgatgaggcc tgctaaacag caaaacggag
     3661 acgcgtgatg gctattattc tcggcattga cccgggttcg cgcgtgaccg gctatggcgt
     3721 catccgccag gtaggcaggc aactgtccta cctgggtagc ggatgcatac gcaccaaggt
     3781 ggatgattta ccgtctcgtc tgaagctcat ctatgcgggc gtgacggaaa tcatcaccca
     3841 gttccagcca gattatttcg ccattgaaca agtctttatg gccaaaaatg ccgactcggc
     3901 tctgaaactg gggcaggcgc gcggcgtggc gattgtggcg gcggtgaatc aggaattgcc
     3961 ggtatttgaa tatgcggcac gtcaggtaaa gcaaacggtg gtaggtattg gtagtgccga
     4021 aaaaagtcag gtgcagcata tggtccgcac cttgctgaaa ctgcccgcta atccacaggc
     4081 ggatgccgcc gatgcgttag ctatcg
//
LOCUS       DAJTNN010000243         4078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTNN010000243 DAJTNN010000000
VERSION     DAJTNN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.248"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..178
                     /locus_tag="OO150_003921"
     CDS             <1..178
                     /locus_tag="OO150_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5517883.1"
                     /translation="HDVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDIAYEGSGSGD
                     WRTDGFIVGVGYKF"
     gene            243..1650
                     /locus_tag="OO150_003922"
                     /pseudo
     CDS             243..1650
                     /locus_tag="OO150_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            1736..2248
                     /locus_tag="OO150_003923"
     CDS             1736..2248
                     /locus_tag="OO150_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517884.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            2324..2536
                     /locus_tag="OO150_003924"
     CDS             2324..2536
                     /locus_tag="OO150_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5517885.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
     gene            complement(2678..3003)
                     /locus_tag="OO150_003925"
                     /pseudo
     CDS             complement(2678..3003)
                     /locus_tag="OO150_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(3139..3387)
                     /locus_tag="OO150_003926"
     CDS             complement(3139..3387)
                     /locus_tag="OO150_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5517886.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(3742..>4078)
                     /locus_tag="OO150_003927"
     CDS             complement(3742..>4078)
                     /locus_tag="OO150_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5517887.1"
                     /translation="VLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRGI
                     DTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARVR
                     SVEEILHAL"
BASE COUNT         1092 a          878 c         1142 g          966 t
ORIGIN      
        1 gcacgacgtg ctgaccggaa gtgatgacgg tcgccacagc aacacgtctc tggcgtgggg
       61 ggctggcgtg cagtttaacc cgaccgaatc cgtggccatt gatattgctt atgaaggttc
      121 cggcagtggc gactggcgca ctgacggttt catcgtgggt gtcggctata aattctgatt
      181 agccaggtaa cacagtgtca tgacagcccg ccggttcagg cgggcttttt tgtggggtga
      241 atatgacagt aaagatttct ggtgtactga aagacggcac aggaaaaccg gtacagaact
      301 gcacaatcca gctgaaagca aaacgtaaca gtaccacggt ggtggtgaac acgctggcct
      361 cagaaaatcc ggatgaagcc gggcgttaca gtatggacgt tgagtatggt cagtacagcg
      421 ttattctgtt ggtggaggga ttcccgccgt cacatgccgg gactatcacc gtgtatgaag
      481 attctcaacc cggtacgctg aatgattttc tcggtgccat gactgaggat gatgcccgtc
      541 cggaggcact gcgtcgcttt gaactgatgg tggaagaggt ggtgcgtaac gcagaggagg
      601 cgaagaagaa tgccggagag gcggagacgt cagcgaggaa tgccggcata tcagccagtc
      661 aggcagaaga gagcgctgca aatgctgaca cttcagcagg ggatgcatcg gagtcagccc
      721 ggcaggcggc agaaagtgca gcctcagcaa agcagtcaga ggaggcgtcc tcgtcctcgg
      781 cctctgcggc cgctcaaaaa gccagtgagt cattacaaag tgcaacagat gctgagttgt
      841 caaaaaagac ggcagaaagt gcagccggta atgcagtcag ggatgcaacg accgcaacag
      901 aaaaagcccg ggagtcagca gaaagcgcac agtcagcgga acaaagcagg atagcggcgg
      961 aagaggccgt aaaccgaatc cccaccgtgg tgggacctcc cgggccaaag ggggaaccgg
     1021 ggcccgcggg tcctcagggg ccgaagggag ataaaggaga gcgtggcgac accggcccgg
     1081 caggggccaa aaggtgacaa aggcgatccg ggagtggctg gaccagcagg tccggcaggt
     1141 gcgccggggc cgaaaggcga taaaggtgat ccgggagtag caggtccagc aggtccggcg
     1201 gggccacagg gaccgaaggg agacacagga gcccccgggc aaggaacaga actgcttact
     1261 actgccaata catggactca ggcacaaact tttaatggtg gtattaatgg caatttgacg
     1321 gtgaccggaa acggctcatt taacgatatt cagatccgtt cggataaacg taacaagcga
     1381 aatctggtaa aactggataa tgcgttagat cgtctggagg cacttactgg ttatctttac
     1441 gagatacagt actctgccga cggttggcaa acgtcggttg gtttaattgc tcaggatgca
     1501 caaaaagcat tgcctgaact ggtaactgaa gacgcagacg ttatatctgg tgaaaaacgt
     1561 ctgcgtctta actacaacgg cataattgca ttgttagtcg agggctttaa aacacttcgt
     1621 catgagatta aagaactccg ggagaagtaa acgacagctg ttgtagtttc tggtttctac
     1681 tgaatttaaa ttgtggggtg acactcaccc cacgcattca gaagggggag atgagatggg
     1741 ggtaacatcg ggatgggtag gctcttcggc taagagcgaa acaggtgagc agtggatggg
     1801 agccgccgga accaaactgg gactgagtaa accttttatg atgagccaaa tggttgggcg
     1861 aactatgggc tgtaaaattg caactgagta ctataaatgg aaatcctctg acaaggttga
     1921 taactggggc gcagttggcg ctgactggcc tttagaagaa aaaagtaaag gtacaattac
     1981 aaacgccgca agctgcggat cagggaggct ggtaggggct gtcgttacac tttctcactt
     2041 tttgacgaac tctacaccga cagctgctgt ttatttagcc ggtggtaaag caggtaacat
     2101 caccgtaaac gtaggtggtg ctacacaaac catgatttat cagggcgttg ttagtgggtt
     2161 ccagtattac tggtcaggtt ctgttagttc cgctttcgtg gaggcaatga aaaagacggg
     2221 agtaccccag gatctaaaaa ttagttaaat ggtaaataaa taattttaaa aacttcacgt
     2281 tctacatacc ggagacgccg gatatatagg atattgtttt aagttgccag agaaattttt
     2341 ccggacggat gctgataata atgatgtgcc aatgacggca gcatcatgga tggcactgag
     2401 tgaagcgaca gagcaggcga tgtttgcgaa gggagtggag attaatacac ggcagttgca
     2461 gatgaaagcc gaggttgagg cgctgacaga cctgaaggcg atccgcagtt atgttgtcgg
     2521 atggcctgct ggctgaataa aaaaacggga ccacgaccag tcccggaacc atgagtttta
     2581 gggtattagt ttgttatcat agttagcgtg ctaagtatgc catgtcaggt tgattagtga
     2641 agtgatgttg ttcgcatttt tgcacggcgg agaatattca gatttttgga aatccatatt
     2701 tttcccgtgc gcgattacat gcttcattta cgatgctatt ttcgccggac attgcgaatt
     2761 ccctgcatgt ggacggtttt tgtaaacaga gcaatatgcg ttttctccgg ggggggccgg
     2821 caagagcgcc acatcgggga tttttctgat tggtgccgcg catacagcga tgaaaaggag
     2881 atatttgctc agtgagactg accgaaacct ttccgccagc atcgtcagct tctgcccagt
     2941 aaaaagagac gcggaaaatg cacaacaggc accacacgtc atgcatggat tcagattgct
     3001 cataattcac cagtacagct ataaatcgta aagagaacag cggtacatcg tatgtaagaa
     3061 cgtaccgcgg ctggctggtg aacttccgat agtgcgagta ttgaatgatt tccagccgtt
     3121 accgatttta cgtgctaatt agtgaacaaa ccactcgtca gcagactccc aggtatcttt
     3181 cagagtttcc tgaacaaaag ttttagctga atctttatcg gcggtgcgcg taacagaaag
     3241 gccatcgttg ctggtagctt ttacgatcac ctctacatcg tcataacgtt tactgatgcg
     3301 tcgggttaat tcttccttta acgcatccac agcaccggtt ggcattttag tcattttttc
     3361 tttggctatg cagatttcaa tacgcataaa agtccctcta cactgtgttt gtatacagta
     3421 tcatttttaa ctgtatggat aaacagtatt aagggtgaag gaaagtgatt ttttggggca
     3481 tgcatggggc aaaaatattt gttgtggggc attttggggc atgattggga cactttgtta
     3541 catatgaact ttgccgattt ttatatgaca tggaaataca acgctttgat tcttctaaat
     3601 aaaaaacatg ctcttgggcg ttctttagtg atttttaaaa attccgtatc acgcagttaa
     3661 agtggcgggc atactcttca aggctggtga tccccaaccg cacccattta ggatgcgacc
     3721 attgcggtag accgatgtaa atcataacgc gtgaaggatc tcttccacgc tacgcacacg
     3781 ggcgatgcgc gggtagatat gattaatgct gttattgtgt tgctcggcgc tggcggcact
     3841 acaggcgtct tcggcaatca ccagattaaa gccaagttcc caggcattgc gggcggtgga
     3901 ttcaacaccg atattggtcg agatcccaca taacactatt gtatcgatac ctcggcggcg
     3961 cagttgcaac tccagatccg taccgtaaaa cgcaccccac tgacgtttga tgatttcgat
     4021 atcactgtca gttgcaccta atgcagctgg atgttgccac cagttttcgg gcaacact
//
LOCUS       DAJTNN010000244         4064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTNN010000244 DAJTNN010000000
VERSION     DAJTNN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.249"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1042)
                     /locus_tag="OO150_003928"
     CDS             complement(<1..1042)
                     /locus_tag="OO150_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5517888.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSR"
     gene            1533..1643
                     /locus_tag="OO150_003929"
     CDS             1533..1643
                     /locus_tag="OO150_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5517889.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            1923..2918
                     /gene="pgl"
                     /locus_tag="OO150_003930"
     CDS             1923..2918
                     /gene="pgl"
                     /locus_tag="OO150_003930"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5517890.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2959..3911)
                     /locus_tag="OO150_003931"
                     /pseudo
     CDS             complement(2959..3911)
                     /locus_tag="OO150_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1088 a          828 c          952 g         1196 t
ORIGIN      
        1 cccgggaatt ctcctcggtg gaaaaagaag tccattgtgt agaaacctcc cgacgatctg
       61 cttcaggaaa ccaggctgcc acagtttcgg gaagaggggg gatctgaaag ttctgccccc
      121 cttcagtgat gactctgata tggcgcgaat gtaaattttg cggcaacatc tggcgtaaaa
      181 acagtctgtt ttggtctgac agggaaatac gaaactgagg cccgttaaaa tttggaaccg
      241 aagtttgctg tattaagaat agcagagttt gatctgataa ggggttattt gcaatctcaa
      301 tagttatatg aggatctaaa cgaaaaagat ttgtcgggat tgtagttatt tggttgtttt
      361 caaaataaaa aaatcttctc atgataatat tttcttggat gtttggtaag ttgcttaatc
      421 tattgctata tgcaaaaaga agttgcagtc tcctaggtaa agggggcagt tcagtaagat
      481 ggttgtgcgc aaccttaagt atcttaagat tagaaggcag agatggaaga gtttgcagtt
      541 cgttgttcct aacctcaagt ttttttaaat tcaagggtaa tgaaggcagt gtggataacc
      601 tattatactc tgcgcttagt gatttgatgg tgtctggtaa taacggcaga ttctctaatt
      661 gattttcgga cacatccaaa gttattagat aatgtggtaa tgcagatata tgtgttaaga
      721 aattatctct tgcaataagt tctttaatat tttcaggtaa atcagtaagg atggttaatt
      781 tattacagct cacatcaagc tttttaatgc aagcagggag tacaggtaac gaggaaagcg
      841 ccaaggctcg caggtccagt ttatgcccct tagaggtaag acatgcacga agtcttctta
      901 ccgcctcagt acgctgttct tccggtaacc cctgtttctc ccaatcatcc cattcggaca
      961 aataatggtt gggggatgta attgtgctga tatgtggaaa taaacaggat gaaatatttc
     1021 tgagcatatt aatttctcgc atatggatat ggaattagat tgtgaataaa ttgtatttaa
     1081 tgtatctctc ctaatgagga gggtgtttca gaatattcgc cataagggaa tgagtgttat
     1141 atcatgcttt ctatcctggt aagtatattt gcttttttcg tatatttggt tcatcttttt
     1201 ttgatgtttt acatattcac aatgcagagg atttatacca gaagacatta gtcttgagta
     1261 agatactaca acatctccaa ctattttagc cagcctgact acttgacttg gtaatatttg
     1321 ttgctaataa tcatgtataa aataagctta tatacatagt aacttcagca aaggatttag
     1381 taatgatcca aatcatatat caatgtactg tttttttctt taaaaaaaca gtacattcta
     1441 ttttataatg actgtgtatt cccatggaag gagatcaccc aattatcccc cccgactttt
     1501 gacgcagtgt cttgttaaca gggggattta ccatgcttat tggctatgta cgcgtatcaa
     1561 caaatgacca gaacaccgat ctacgacgta atgcgttgaa ctgtgcagga tatgagctga
     1621 ttgttgagcg aatgggagct tgattggaag ttgttagggt atggtgttta cggtcgttga
     1681 tatggcgaac aaacgcgggg agcgtcacgc tccctaaatg gtcgcgaaaa tatctgtggc
     1741 attgtcaaat ttgtgacggt gactaatatt ttaatgatat gccattcgaa atggtgtgct
     1801 aacgtattga tttaattgtg acgcgatgtt gtattttatc gcgttttgtt gtctgttttt
     1861 cattcgcttg atgttgctat ttacatgaaa aataaagaat ttattgtttt tgaggtgtgt
     1921 ttatgaagca aacagtttat atcgccagcc ctgagagcca gcaaattcac gtctggaacc
     1981 tgaatcatga aggcgcactg acgctgacac aggttgtcga tgtgccgggg caggtgcagc
     2041 cgatggtggt cagcccggac aaacgttatc tctatgttgg tgttcgccct gagtttcgcg
     2101 tcctggcgta tcgtattgcc ccggacgatg gcgcactgac ctttgccgca gagtctgcgc
     2161 tgccgggtag tccgacgcat atttccaccg atcaccaggg gcagtttgtc tttgtaggtt
     2221 cttacaatgc gggtaacgtg agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg
     2281 tcgatgtggt cgaggggctg gacggttgcc attccgccaa tatctcaccg gacaaccgta
     2341 cgctgtgggt tccggcatta aagcaggatc gcatttgcct gtttacggtc agcgatgatg
     2401 gtcatctggt ggcgcaggac cctgcggaag tgactaccat tgaaggggcc ggcccgcgtc
     2461 atatggtatt ccatccaaac gaacaatatg cgtattgcgt caatgagtta aacagctcag
     2521 tggatgtctg ggaactgaaa gatccgcacg gtaatatcga atgtgtccag acgctggata
     2581 tgatgccgga aaacttctcc gacacccgtt gggcggctga tattcatatc accccggatg
     2641 gtcgccattt atacgcctgc gaccgtaccg ccagcctgat taccgttttc agcgtttcgg
     2701 aagatggcag tgtgttgagt aaagaaggct tccagccaac ggaaacccag ccgcgcggct
     2761 tcaatgttga tcacagcggc aagtatctga ttgccgccgg gcaaaaatct catctcatct
     2821 cggtatacga aattgttggc gagcagggac tactgcatga aaaaggccgt tatgcggtcg
     2881 ggcagggacc aatgtgggtg gtggttaacg cacactaacc gctgatttaa ccggcgcagt
     2941 ctctcctgcg ccggtgtatt aacctatctc ctgtaacgca tgtctctggc gttcgacgat
     3001 attggtccac aaattgtctt tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg
     3061 ttcagctgcc ggagaaagca cggcatcttt gcggcgaata atccccaatg tccgacgtat
     3121 aacgggttcc accagtggga taccaataat cgaagaatag ggcgcatggg gcattgccag
     3181 accaggaagc gccgaaatcc ccagtcctgc ctccactagt cctaatgacg tcgaaagatg
     3241 acgcacttcg taaaaccagt ccagcttcca gggcttgtcg gccagctgtt gttctatcaa
     3301 cagtcggttg ccgctggagg agcgtacgcc aatcattttg taacccacta actcctgcca
     3361 ttctacaagc tgctttttgg ccaatggatg gtcacgccga caggcagcac gaacggctcg
     3421 ttgaccagtg gggtaaaatc aatggatgaa tttgtgacgt tgttcatgtt tatgccaaag
     3481 tctgattcgt tacacagcac cgattccatg caattattcg tgccttgttc cagaatccgc
     3541 actttaatat tgggatacag ctcattaaat ttaccgattg ccagcggtaa aaaataaaat
     3601 actgcggtcg gaatacacgc taacgtcacc ataccacgat gataagcatt catatcacga
     3661 atattaaaaa gcgtttcatc gaatttcttt attaattccc gcgcctccgg gagcaacctt
     3721 tttcctgctt ttgttaacgt aaccttgcga gttgtacgct caaatagttg aatatgtaaa
     3781 tcttcctcca tcttttttat tcttcgcgtt aatgcaggtt gcgtaatatt gagtaattta
     3841 gcggcattgt taaacgaact ggactctgcc agtatgacaa atgccttcat acttgataat
     3901 tcatgcttca ttattactcc ggaaaatgga agcgacgatt ttgggtggct ggccgttaaa
     3961 aattttaact gcatttagcc aacttaaatt aatgaaaaaa tgttattaat cgttgagcta
     4021 aagtcattag agatgctttg cccttaatgt aaccatatcg caat
//
LOCUS       DAJTNN010000245         4022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTNN010000245 DAJTNN010000000
VERSION     DAJTNN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.250"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..489
                     /locus_tag="OO150_003932"
     CDS             <1..489
                     /locus_tag="OO150_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5517891.1"
                     /translation="PAIIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAP
                     TLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPE
                     GDVPGLVKGNTYLTPQQQTAELTGPVNKVIIDTAQFLKEQGKVPAVANDYSQYVTSRF
                     VQ"
     gene            502..1269
                     /gene="tauB"
                     /locus_tag="OO150_003933"
     CDS             502..1269
                     /gene="tauB"
                     /locus_tag="OO150_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5517892.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1266..2075
                     /gene="tauC"
                     /locus_tag="OO150_003934"
     CDS             1266..2075
                     /gene="tauC"
                     /locus_tag="OO150_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5517893.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTP"
     gene            2090..2941
                     /gene="tauD"
                     /locus_tag="OO150_003935"
     CDS             2090..2941
                     /gene="tauD"
                     /locus_tag="OO150_003935"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5517894.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGITAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
     gene            complement(3047..4021)
                     /gene="hemB"
                     /locus_tag="OO150_003936"
     CDS             complement(3047..4021)
                     /gene="hemB"
                     /locus_tag="OO150_003936"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5517895.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT          866 a         1054 c         1193 g          909 t
ORIGIN      
        1 cccgcgatta tcgccgcatg gcagcggggt gatattgatg gtgcttatgt ctgggcaccg
       61 gcggttaacg ccctggaaaa agacggcaag gtgttgaccg attctgaaca ggtcgggcag
      121 tggggtgcgc caacgctgga cgtctgggtg gtgcgcaaag attttgccga gaaacatcct
      181 gaggtcgtga aagcgttcgc taaaagcgcc atcgatgctc agcaaccgta cattgctaac
      241 ccagacgcgt ggctgaaaca gccggaaaac atcagcaaac tggcgcgttt aagcggcgtg
      301 ccggaaggtg acgttccggg gctggttaag gggaatacct atctgacgcc gcagcaacaa
      361 acggcagaac tgaccggacc ggtgaacaaa gtaatcatcg acaccgcgca gtttttgaaa
      421 gagcagggca aggtcccggc tgtagcgaat gattacagcc agtacgttac ctcgcgcttc
      481 gtgcaataaa aggaggcgca gatgctgcaa atctctcatc tctatgccga ttatggcggc
      541 aaaccggcac tggaagatat caacctgacg ctggaaagcg gcgagctact ggtggtgctg
      601 gggccgtccg gctgcggtaa aaccacgttg ctgaatctga ttgccggttt tgtgccttat
      661 cagcatggca gcattcaact ggcgggtaag cgtattgagg gaccgggagc agagcgcggg
      721 gtggtttttc agaatgaagg gctgctaccg tggcgcaatg tacaggacaa cgtggcgttc
      781 ggcctgcaat tggcaggtat agagaaaatg cagcgactgg aaatcgcgca ccaggtgctg
      841 aaaaaagtgg ggctggaagg cgcagaaaaa cgctatatct ggcagctttc cggtggtcaa
      901 cgtcagcggg tggggattgc ccgcgcgctg gcggcgaatc cccagctgtt attactcgac
      961 gaaccgtttg gtgcgctgga cgccttcacc cgcgaccaga tgcaaactct gctgctgaaa
     1021 ctctggcagg agacgggcaa gcaggtgctg ttgattaccc acgatataga agaagcggtg
     1081 tttatggcga ctgaactggt tctgctttca tccggccctg gccgtgtgct ggagcggctg
     1141 ccgctcaact ttgctcgccg ctttgttgcg ggagagtcga gccgcagcat caagtccgat
     1201 ccacaattca tcgccatgcg cgaatatgtt ttaagccgcg tatttgagca acgggaggcg
     1261 ttctcatgag tgtgctcatt aatgaaaaac tgcattcgcg gcggctgaaa tggcgctggc
     1321 cgctctcgcg tcaggtgacc ttaagcattg gcacgttagc ggttttactc actgtatggt
     1381 ggacggtggc ggcgctgcaa ctgattagcc cgctgttttt gccgccgccg caacaagtac
     1441 tggcaaaact gctcaccatt gccggaccgc agggatttat ggacgccacg ctgtggcagc
     1501 atctggcagc cagcctgacg cgcattgtgc tggcgctact cgcggcggtg gtgatcggta
     1561 ttccggtcgg gatcgccatg gggctaagcc cgacggtgcg cggcattctg gatccggtaa
     1621 tcgaacttta tcgtccggtg tcgccgctgg cttatttgcc gctgatggtg atctggtttg
     1681 gtattggtga aacctcgaag atcttactga tctatttagc gatttttgca ccggtggcga
     1741 tgtcggcgct ggcgggggtg aaaagcgcgc agcaggttcg cattcgtgcc gcccagtcgc
     1801 tgggtgccag ccgtgcgcag gtgctgtggt ttgtcatttt gcccggcgcg ctgccggaaa
     1861 tcctcaccgg attacgcatt ggtctgggtg tgggctggtc tacgctggta gcggcggagc
     1921 tgattgccgc gacgcgcggt ttaggattta tggttcagtc agcgggtgaa tttctcgcga
     1981 ctgacgtggt gctggcggga atcgcggtga ttgcgattat cgcctttctt ttagaactgg
     2041 gtttgcgcgc gttacagcgc cgtctgacgc cctagcatgg agaagtacaa tgagtgaacg
     2101 tctgagcatt accccgctgg ggccgtatat cggcgcacaa atttcgggtg ccgacctgac
     2161 gcgcccgtta agcgataatc agtttgaaca gctttaccat gcggtgctgc gccatcaggt
     2221 ggtgtttctg cgcgatcaag ccattacgcc gcagcagcaa cgcgcgctgg cccagcgttt
     2281 tggcgaactg catattcacc ctgtttaccc tcatgccgaa ggggttgacg agatcatcgt
     2341 gctggatacc cataacgata atccgccaga taacgacaac tggcataccg atgtgacatt
     2401 tattgaaacg ccacccgcag gggcgattct ggcagctaaa gaattacctt cgaccggcgg
     2461 tgatacgctc tggaccagcg gtattacggc ctatgaggcg ctctctgttc ccttccgcca
     2521 gctgctgagc gggctgcgtg cggagcatga tttccgtaaa tcgttcccgg aatacaaata
     2581 ccgcaaaacc gaggaggaac atcaacgctg gcgcgaggcg gtcgcgaaaa atcctccgtt
     2641 gctacatccg gtggtgcgaa cgcatccggt gagcggtaaa caggcgctgt ttgtgaatga
     2701 aggctttact acgcgaattg tcgatgtcag cgagaaagag agcgaagcct tgttaggctt
     2761 tttattcgcc catatcacta aaccggagtt tcaggtgcgt tggcgctggc aaccgaatga
     2821 tattgcgatt tgggataacc gcgtgactca gcactatgcc aatgccgatt acctgccaca
     2881 gcggcggatt atgcatcggg tgacgattct tggggataaa ccgttttatc gggcggggtg
     2941 atacgagtgc ggacggttcc ctcgccccct tggggagagg gttagggtga gggggcgttc
     3001 accgtacttt caacaggtta actccccctt tctgagagga aaaaaattaa cgcagaatct
     3061 tcttctcagc caaatccagc gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa
     3121 ttgaacctaa gctttcgagc acgactttct cttcatctat agcacccgcc agcgcggcga
     3181 acttaatcat cgcgtactca ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt
     3241 cacgcagctc acgcacgatg tcgaggtacg ctccagcagg tttaaccatc aggcagtctg
     3301 cgccctgggc ttcatccagc agtgactcac gaatcgcctc gcgacggttc atagggttca
     3361 tctgatagct tttgcggtcg ccttttaatg cgcttccggc agcttcacgg aacgggccat
     3421 aaaaggaaga ggcgaacttg gtcgaatacg acataatcgc cgtatcttta aagcctgcag
     3481 cgtccagcgc ctgacgaatc gcctgtacct ggccgtccat cgcggcggaa ggggcgatga
     3541 agtctgcgcc tgcagcagct gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt
     3601 cgttgtcgac gccatgctcg cacagcacac cgcagtgacc gtgagaagtg tattcacaga
     3661 aacaagtgtc tgacatgacg atcatttctg gcacagtctg cttgcagatg cgcgacatac
     3721 gcgccaccag tccatcttcc cgccaggcgt cgctgccggt ttcatcggta tggtgagaga
     3781 tgccgaaagt catcacggaa cgaataccgg cgttggcgat gcgttcaatt tcgcgtgcca
     3841 gatgtttctc tggaatgcgc atcacgcctg gcatggcttc aacggctttg tagtcgtcaa
     3901 tttcttcttc aacaaagatc ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa
     3961 acatagcgcg cagcgcagga gatttgcgca ggcgacgagg gcgttggatt aagtctgtca
     4021 tg
//
LOCUS       DAJTNN010000246         3981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTNN010000246 DAJTNN010000000
VERSION     DAJTNN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.251"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            51..380
                     /locus_tag="OO150_003937"
     CDS             51..380
                     /locus_tag="OO150_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5517896.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            628..873
                     /gene="nrdH"
                     /locus_tag="OO150_003938"
     CDS             628..873
                     /gene="nrdH"
                     /locus_tag="OO150_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5517897.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            870..1280
                     /gene="nrdI"
                     /locus_tag="OO150_003939"
     CDS             870..1280
                     /gene="nrdI"
                     /locus_tag="OO150_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5517898.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1253..3397
                     /gene="nrdE"
                     /locus_tag="OO150_003940"
     CDS             1253..3397
                     /gene="nrdE"
                     /locus_tag="OO150_003940"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5517899.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENVQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3407..>3981
                     /locus_tag="OO150_003941"
     CDS             3407..>3981
                     /locus_tag="OO150_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5517900.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRD"
BASE COUNT          964 a         1022 c         1029 g          966 t
ORIGIN      
        1 aaaattatgg tctacactga aaattacatc gaattctaat gggggatcat atgtttaacc
       61 gaccgaaccg caacgacgtt gatgatggcg tgcaggatat ccagaatgat gtcaatcaat
      121 tagctgacag cctcgaatct gtattgaagt cctggggcag cgacgccaaa ggggaagctg
      181 aagccgcacg cagtaaagct caggcgttgc tgaaagaaac cagggcgcga atgcatggtc
      241 gtactcgcgt ccagcaagcc gcgcgcgatg ccgttggctg cgcggattct tttgttcgtg
      301 aaagaccctg gtgtagcgtg ggtacagcag ctgcggtagg tatttttatc ggcgcactgt
      361 taagcatgcg caaatcgtag tgcaaaaatg ataataaata cgcgtctttg accccgaagc
      421 ctgtcttcgg ggtttctttt tgcctggtga atcacaaaaa tccccctccc ccgtcacgct
      481 catatccagg gtaatttcga tcactatttg ctatatattg tgtagttgaa tcttttttca
      541 actacatcta gtgtctctgt atcaacagag agacaacccg acgcgtatca tcgcgccgta
      601 tcttcatttt aaacggaaat acgaatcatg cgcataacta tttacactcg taacgattgc
      661 gttcagtgcc acgccaccaa acgggcgatg gaaaaccggg ggtttgattt tgaaatgatt
      721 aatgtcgatc gcgttcctga agcagcggaa gcgttgcgtg ctcagggctt tcgtcagttg
      781 ccggtagtga ttgctggcga tcttagctgg tctggtttcc gtccggacat gattaaccgt
      841 ctgcatccag cgccacacgc ggccagtgca tgagccagct cgtctacttc tccagcagct
      901 ccgaaaacac gcagcgtttt atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca
      961 atgagcggga acggattcag gtagacgagc cttacatcct gatcgtgccc tcttacggcg
     1021 gcggcggtac ggctggcgcg gtgccacgac aggtaattcg ctttttaaac gacgagcaca
     1081 accgggcgtt gcttcgcggc gttattgctt ctggtaatcg caactttggt gaggcgtatg
     1141 gccgcgccgg agatgtgatt gcccggaaat gcggcgtgcc gtggctgtac cgttttgaac
     1201 tcatgggtac gcaaagcgat atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac
     1261 gacaaccgca gaatgcctga cgcaggaaac gatggattac cacgcgctga atgcgatgct
     1321 taacctctac gatagcgcag gtcgcattca gttcgataaa gaccgccagg ccgttgacgc
     1381 ctttatggcg acgcatgtgc gtccgaacag tgtgaccttc agtagccagc agcagcgcct
     1441 gaactggctg gtcaacgaag gttactatga tgaaagcgtt cttaatcgct actctcgcga
     1501 ctttgtcatt acgctgtttg cctacgcaca caccagcggt tttcgtttcc agacattcct
     1561 cggggcatgg aagttttaca ccagctatac gttgaagaca ttcgacggta aacgttatct
     1621 ggaagatttt gccgatcgag tcacgatggt ggcgctgacg ctggcacaag gcgatgcgac
     1681 gctggcgttg caactgaccg atgaaatgct gtcaggacgc tttcagccag ccacgccgac
     1741 attcctcaac tgcggtaagc agcagcgcgg cgaactggtt tcctgttttc tgctgcgtat
     1801 tgaagacaat atggagtcga ttggccgggc ggtaaattcc gcactgcaac tgtcgaaacg
     1861 cggcggcggc gtggcatttt tgctgtcgaa tctgcgagaa gcgggcgcgc caattaaacg
     1921 tattgaaaat caatcttctg gcgtaattcc ggtgatgaaa atgctggaag acgcattttc
     1981 ctatgccaac caactcggcg cgcgtcaggg ggcaggtgca gtctatttac atgctcatca
     2041 tcccgatatt ctgcgttttc tcgatacaaa acgggaaaat gccgacgaaa aaatccgcat
     2101 taaaacactg tcgctgggcg tggtgatccc ggatatcact ttccatctgg caaaagagaa
     2161 tgtgcagatg gcgctgtttt cgccctatga cgttgagcga gtttatggca agccgtttgc
     2221 cgatatcgcc atcagcgaac actatgacga actggttgcc gatgaacgca tccgcaaaaa
     2281 atacctcaac gcccgtgatt tcttccagcg gctggcagaa atccagtttg agtccggcta
     2341 tccctacatc atgtatgaag acacggtaaa ccgtgctaac cctgtcgccg ggcgcataaa
     2401 tatgagtaat ctctgctcag agattttgca ggtcaactgt gcctcagagt atgacgagaa
     2461 tctcgactat gcccgcacag gccatgatat ctcctgcaat ttaggttcgt tgaatattgc
     2521 gcacaccatg gattcccccg attttgcccg cacggtagag actgccgtgc gcggtttaac
     2581 ggcggtgtca gatatgagtc atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc
     2641 ctcacacgcc atcggactgg ggcagatgaa tttacacggc tatctggcgc gagaaggcat
     2701 cgcttatggt tcgccggaag cactggattt caccaatctc tatttctatg ccatcacctg
     2761 gcacgcactg cgtacctcga tgttgctggc gcgcgaacgc ggtgaaacct tcgccgggtt
     2821 caaacagtca cgttatgcca gtggtgaata ttttagccaa tatctgcaag gtaactggca
     2881 gccgaaaacg gcgaaagttg gcgaactgtt tgcccgcagc ggtattacgt tacctacccg
     2941 tgagatgtgg gcgcaactgc gcgacgacgt gatgcgctac ggcatttata accagaatct
     3001 tcaggcggtg ccgccaaccg gttctatctc ttatatcaac catgctacgt cgagtattca
     3061 tccgattgtg gcgaaagtag agatacgcaa agagggcaaa acaggacgcg tttactaccc
     3121 tgccccgttt atgactaacg agaatctggc gctgtatcag gatgcttacg aaatcggcgc
     3181 agaaaagatc atcgacacct acgccgaagc gactcgccat gtcgatcagg ggctgtcgct
     3241 gacgctgttt ttccccgata ccgccaccac tcgcgatatc aacaaagcgc agatttacgc
     3301 ctggcgcaag ggtatcaaaa cgctctatta cattcgcctg cgtcagatgg cgctggaagg
     3361 cactgaaatt gaaggctgcg tctcctgtgc actttaagga atatctatga aactctcacg
     3421 tatcagcgcc atcaactgga acaagatatc tgacgataaa gatctggagg tgtggaatcg
     3481 cctgaccagc aatttctggc taccagaaaa ggtgccgctg tcgaacgata ttcctgcctg
     3541 gcagacatta actgtcgtag aacaacaact gacgatgcgc gtttttactg gcctgacgct
     3601 gctcgacacg ctgcaaaatg ttatcggcgc gccttcgctg atgccctatg cactcacgcc
     3661 tcatgaagaa gcggtattat cgaatatcag ctttatggaa gcggttcatg cccgctctta
     3721 cagttcgatt ttctcgacgc tgtgtcagac caaagatgtc gatgccgcct acgcctggag
     3781 tgaagaaaac gcaccgttgc agcgaaaagc tcagattatt cagcaacatt atcgcggtga
     3841 tgatccgctg aaaaagaaaa tcgccagtgt gtttcttgaa tcttttttgt tctattccgg
     3901 tttctggctg ccgatgtatt tttccagccg cggaaagctg accaataccg cagatctgat
     3961 ccgtctgatt atccgcgatg a
//
LOCUS       DAJTNN010000247         3959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTNN010000247 DAJTNN010000000
VERSION     DAJTNN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.252"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            115..618
                     /gene="yeaK"
                     /locus_tag="OO150_003942"
     CDS             115..618
                     /gene="yeaK"
                     /locus_tag="OO150_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5517901.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(619..723)
                     /locus_tag="OO150_003943"
     CDS             complement(619..723)
                     /locus_tag="OO150_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517902.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            892..1338
                     /locus_tag="OO150_003944"
     CDS             892..1338
                     /locus_tag="OO150_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5517903.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1295..2116)
                     /gene="nimR"
                     /locus_tag="OO150_003945"
     CDS             complement(1295..2116)
                     /gene="nimR"
                     /locus_tag="OO150_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR5517904.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2213..3409
                     /gene="nimT"
                     /locus_tag="OO150_003946"
     CDS             2213..3409
                     /gene="nimT"
                     /locus_tag="OO150_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR5517905.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            3449..3796
                     /locus_tag="OO150_003947"
     CDS             3449..3796
                     /locus_tag="OO150_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5517906.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3818..>3959)
                     /gene="yoaF"
                     /locus_tag="OO150_003948"
     CDS             complement(3818..>3959)
                     /gene="yoaF"
                     /locus_tag="OO150_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5517907.1"
                     /translation="VYCQQKGGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAG
                     EK"
BASE COUNT          904 a         1015 c          993 g         1047 t
ORIGIN      
        1 ttacatgatg cgtataaagc ttccgatgag cgtttatatg tcaataagca gaacaaaaac
       61 agccgttcat gataaccttc tgtggttgtt tgcttgtaat ctcaggagcg tgaaatgact
      121 gaaatggcta aaggaagcgt gacgcatcag cggttaatcg ctttattatc acaagaaggt
      181 gctaacttcc gcgtcgttac ccatgaagcg gtaggtaaat gtgaagcggt gtcagaaatt
      241 cgtggcaccg ctttaggcca gggagctaag gcactggtat gtaaagtcaa aggcaatggc
      301 gtaaatcaac atgttctggc gatcctcgct gccgatcaac aggccgatct gagccaactt
      361 gccagccata taggcggatt acgcgcttcg ttagccagcc ctgctgaagt cgatgaactc
      421 acaggctgtg tcttcggcgc gatcccccct ttcagcttcc atccaaaact caaactggtt
      481 gccgaccctc tactcttcga acgatttgat gaaatcgcct tcaatgcagg gatgctggat
      541 aaatccgtta ttttgaaaac cacggattat ttgcgcattg cgcaaccaga actggtcaat
      601 ttccgccgca ctgcgtaact agcctgtccg ttcaataagc agaacggaca aaaccagcac
      661 aacagcaatg gcaaaaaacg atgacgtgat aatcagtgtc tcgacgaaca tttgatcgtt
      721 catagcatgc ccccgggaga tagctgtttt cacgttattg ttagcgtgca caaatggcag
      781 tttgatgaca gttcgcctga tttttatgca aaaaacgtaa agattttcta cttccttcct
      841 gcagcaagcg taaagtaagc aggcttatta ttttttggca aggaaaccac gatgtttgat
      901 gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca taacaccact
      961 gtcgccgttt caattctggt gttgatcatt gtccgcgtca caccgttaag cacctttttc
     1021 ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat tggcgttatg
     1081 gcccccatcg ccagcgggac gctaccgccc tcaaccttga ttcactcgtt tctgaactgg
     1141 aaatcgctgg tggcaattgc agtaggcgtg attgtctcct ggctgggtgg gcgcggcgtg
     1201 accttaatgg gcagccagcc gcaactggtc gccgggctgc tggtaggcac tgttttaggc
     1261 gtggcgctgt ttcgcggcgt accggtcggc ccgcttattg ccgcgggtct ggtttcgctg
     1321 attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt
     1381 tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga gcaactttct
     1441 gtacagtatc gcctttcact aatccctgaa gtgccataat cagctgtaac tgctgacgcc
     1501 actggcgaaa actcaacccc gtctctttta cgatcagtcg cgcaagattg cgttcactca
     1561 tcgcaaaaaa gccagcccat tgccctaatg acccccactc gacgggccct ttcgccatca
     1621 tctccaccat cgtgcggatt ttaggatgag aagaaacggg caggtgcagc tgttgttgtg
     1681 gctgctgagg gagttcatca aacaggacct gtattaatct gcgggtcatc ggctcagcac
     1741 gctgagtcgt cgtcctgttt gccagggtta aaatcaattc ccggcataag ggcgatattt
     1801 tcaatgtgca acatgttgtc ggcattgtca ctgcgctggg ttcgataaac agaaaacaga
     1861 gttcagcgtt agctgtcact tgattgctat gttccacccc gcccggtatc caaaccgcat
     1921 attgcggcgg caccatccac aaagcatttt ccaccgtaca ggtaattgca ccatgtaatg
     1981 ctagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca tccgttccgg
     2041 cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag ccattgagat
     2101 tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag cttgattcaa
     2161 cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc atatgacctg
     2221 ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc tgatgattgc
     2281 cacaacatta cgcgtcacct ttaccggcgc agcaccgtta ctggatacga ttcgttccgc
     2341 ttactcgctg acgacagcgc aaaccggctt attgaccacc ctgccattat tggcctttgc
     2401 gctaatctca cctttggccg ccccggtagc gcgacgtttt ggtatggaac gtagcctgtt
     2461 tgccgcgtta cttttgatct gtgctggtat cgcaattcgc tctctccctt cgccttactt
     2521 attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggcaatg tcttactgcc
     2581 aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg catattccct
     2641 gacaatggga gctgcggcgg cacttggatc ggctatggta gtgccgctgg ctttgaacgg
     2701 ttttggctgg caaggcgcgt tgctcatgct gatgtgtttt cctctgctgg ctcttttttt
     2761 atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc gcgccttaca
     2821 tactcggggt atctggcgtt cgccgcttgc ctggcaggtc acattgtttc ttgggagcaa
     2881 ctcactggtc tattacgtga ttattggctg gcttccggcg atcctcatca gtcacggcta
     2941 tagcgaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag cagctcccgg
     3001 tttgctgatc cctcttttct tacatcatgt gaaagaccag cgtggtattg cagcgttcgt
     3061 tgccttgatg tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc acgcgatcac
     3121 ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt tgacgttcat
     3181 tggtcttcgg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg cacaatccgt
     3241 cgggtatttg ctggcagcct gtgggccgcc gctaatgggt aaaatacacg atgctaacgg
     3301 taactggtcc gtaccactga tgggtgttgc aatactttca ctactgatgg cgattttcgg
     3361 actttgcgcc gggagagaca aagaaattcg ctcatatccg gtgctatagt gacgtgacaa
     3421 atcatgcgtg aaagggagaa caaacacgat gaatattcag tgcaaacgcg tttatgatcc
     3481 ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa
     3541 aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa cggaactgcg
     3601 caaagccttt cacggcgaag tcgtcgattt tacaaccttt cgcgagcaat atcttgcaga
     3661 actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa aacagccgct
     3721 gaccctgctc tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc tggccgactg
     3781 gctacgtagc ttgtgatttt agtacagcat ccggcggtta tttttcacca gccggatggt
     3841 cccgtcgcca caatgcccat tcatcaatcg tttcaccgcc cggtaatttg caattgttgc
     3901 tgaccccttg cgctgtcgac actggaatga gcgtcccgcc cttctgctgg caatagacc
//
LOCUS       DAJTNN010000248         3922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTNN010000248 DAJTNN010000000
VERSION     DAJTNN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.253"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            19..918
                     /gene="pgrR"
                     /locus_tag="OO150_003949"
     CDS             19..918
                     /gene="pgrR"
                     /locus_tag="OO150_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5517908.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1255..2868
                     /gene="mppA"
                     /locus_tag="OO150_003950"
     CDS             1255..2868
                     /gene="mppA"
                     /locus_tag="OO150_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5517909.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMKQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(2919..>3922)
                     /gene="ynaI"
                     /locus_tag="OO150_003951"
     CDS             complement(2919..>3922)
                     /gene="ynaI"
                     /locus_tag="OO150_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5517910.1"
                     /translation="LNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHAVQFLIYTIII
                     SAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDI
                     TSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSG
                     IMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVEN
                     PGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSL
                     NIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT         1099 a          862 c          927 g         1034 t
ORIGIN      
        1 attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc gtttgtcgtc
       61 gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc gcagtcagct
      121 ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct gacgcgaacc
      181 acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct tggccctatg
      241 ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg cccatccggg
      301 acaatacgta ttactactgt agaacatgca gcaaaaacga tattgttacc agcaatgcgc
      361 acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta tggtttgacc
      421 gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat ggataaagat
      481 atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc accggattat
      541 ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca ggcaattaat
      601 ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg tggacgtgaa
      661 gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat cattgatgct
      721 gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg ggctattaaa
      781 gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg ttatcacctg
      841 tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga taggctgaag
      901 tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttctc tcttaacgta
      961 ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg cgacagatgc
     1021 tgattttgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagta ttcggtgttc
     1081 tatgtgcttt gccgcatttt ggcgtcgatt atttacacta aatctgattt aatatattga
     1141 tacataaaac attttatgct tcctgtgtca cttttttgat ggaagttgtt tgcatttctt
     1201 taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa acttatgaag
     1261 cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct ttcgtatgct
     1321 gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg ccacattaaa
     1381 gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat tcaggtcatt
     1441 cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt ccccggcgtt
     1501 gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg cgataacgca
     1561 aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg gcagcgtctg
     1621 gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg aatcaacaac
     1681 gcacaggcga ttattgatgg taaagctacg cctgaccagc ttggcgtcac cgcagttgat
     1741 gcccatacct tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa tttaaccgct
     1801 aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga gtggacgaaa
     1861 cccggaaatc tgatcggcaa tggcgcttat gttcttaaag aacgcgtagt caatgaaaaa
     1921 ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact gcaaaaagtg
     1981 accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc gggggatatt
     2041 gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga tattccgggg
     2101 caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac gcaaaaaggg
     2161 ccgacggcag atcagcgcgt tcgcctggca ttaagtatga cgatagatcg ccgcctgatg
     2221 accgaaaaag tgttgggaac gggcgaaaag ccagcgtggc attttacacc agatgttacc
     2281 gccggattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga actgaatgcg
     2341 caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct gaagctgacg
     2401 cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc atcgatgtgg
     2461 aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac ctatatcgat
     2521 agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga ttataatgaa
     2581 ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc acgctttaac
     2641 aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt taaagcgcgt
     2701 aatgccgatt acaacgcggc agaaaaaatc ctcatgaagc aagcaccgat tgcaccaatt
     2761 tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc tattaataac
     2821 ccggaagatg tggcgtacag ccggattatg tatattgtga agcattgatg tgatgggaac
     2881 tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg ttccggcggt
     2941 gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc gtgtgactgc
     3001 acaatatcga taatcttcaa ataaacgtct tgttgtgcag caagccattc agcccatacc
     3061 gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa ctggttgaaa
     3121 taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat ctcacgtacg
     3181 gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat ggtcgtggta
     3241 atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa cagcgagttc
     3301 ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca gccaatttcc
     3361 gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat actgaaagga
     3421 cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt accggccata
     3481 ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct catgccgaaa
     3541 tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat gcgcgacata
     3601 atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa ctgattaatc
     3661 agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat aaaatcgata
     3721 acgcctgggg tgataaattt gagtttatag ttttctataa cataattaat aatgctacca
     3781 acagcactga taattatggt gtagattaaa aattgcaccg catggaataa aaaacctttt
     3841 cttttacgat ttccgcggcg aaaccaaaag ctcatcagaa tcaatgctgc gcagctaccg
     3901 aaaataatga ccagattaag cg
//
LOCUS       DAJTNN010000249         3853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTNN010000249 DAJTNN010000000
VERSION     DAJTNN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.254"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..440
                     /locus_tag="OO150_003952"
     CDS             <1..440
                     /locus_tag="OO150_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5517911.1"
                     /translation="FTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVR
                     ISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIA
                     EGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            448..1776
                     /locus_tag="OO150_003953"
     CDS             448..1776
                     /locus_tag="OO150_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5517912.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGSYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1823..3148)
                     /gene="rimO"
                     /locus_tag="OO150_003954"
     CDS             complement(1823..3148)
                     /gene="rimO"
                     /locus_tag="OO150_003954"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5517913.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3361..3745
                     /gene="bssR"
                     /locus_tag="OO150_003955"
                     /pseudo
     CDS             3361..3745
                     /gene="bssR"
                     /locus_tag="OO150_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
BASE COUNT         1007 a          857 c          952 g         1037 t
ORIGIN      
        1 gttttaccac catcatgaaa gaaaatggcc tgaaggcgaa cagcctctgt gttgagatca
       61 ctgaaaccgt gatcgagcga attaatgaac atttttatct caatattgaa caactgcgta
      121 aacaaggggt acggatatcg attgatgact ttggcaccgg tttgtcaaac ctgaaacgtt
      181 tttatgaaat taatccagac agcataaagg tggactcgca atttaccggc gatattttcg
      241 gtactgcggg aaaaattgtg cgcattattt tcgatctggc acgctataac cggatcccgg
      301 tgattgcgga aggcgtagag agcgaagacg ttgcacgcga attaattaaa ttaggatgtg
      361 ttcaggctca ggggtatctg taccagaaac ctatgccatt ctccgcctgg gataaaagtg
      421 gaaaattagt aaaagagtag tttacgtatg tccagaatca ataagttcgt acttacagtc
      481 agtctgctga tttttatcat gatttcagca gttgcctgcg ggatctacac tcaaatggta
      541 aaggaacggg tgtatagcct gaaacagtcc gttattgata ctgcttttgc ggtggcaaat
      601 attgctgaat atcggcgtag cgtggcaatt gatcttatca acacgctaaa tcccacggag
      661 gaacagctgt tggttggttt gcgcacagct tacgccgact cggtttcccc ctcttatttg
      721 tacgatgtcg gtccttatct gatttccagt gacgaatgta ttcaggtaaa ggagttcgag
      781 aaaaattatt gtgcagatat tatgcaggtt gtgaagtatc gacatgtcaa aaatacaggg
      841 tttatctctt ttgacggtaa aaccttcgtc tattacctct atccggtaac tcacaatcgt
      901 agtctgatat ttttgcttgg tctggagcgt ttttctttac tgtcaaaatc gctggcgatg
      961 gacagcgaga acctgatgtt ctctctattt aagaacggta aaccggtgac cggtgatgaa
     1021 tataatgcta aaaacgccat cttcaccgtt tcggaagcga tggagcactt cgcctatttg
     1081 ccgaccggat tgtacgtatt tgcgtataaa aaagatgttt atttgcgggt ctgtacattg
     1141 attattttct ttgccgcatt ggtggctgtg atatcgggtg ccagttgcct ctatctggta
     1201 cgcagagtga ttaatcgtgg tattgtggag aaagaagcca tcattaataa ccattttgaa
     1261 cgcgtactgg atggcgggct tttcttttcg gctgccgatg tcaaaaaact ctacagtatg
     1321 tataactcgg cgttcctgga cgacctgacc aaagcaatgg gcagaaaatc ctttgacgaa
     1381 gatttaaaag cgctgccgga aaaaggcagt tatttgtgcc tgtttgacgt cgataaattc
     1441 aaaaacatta acgacacctt cggtcatttg ctgggcgatg aagtgttgat gaaagtggtg
     1501 aaaatcctta aatcacagat cccggtagat aaaggtaaaa tctaccgctt cggcggtgac
     1561 gaatttgcgg tgatttatac gggtggaacg ctggaagagt tgctatcgat tctaaaagaa
     1621 atcgttcatt tccaggtggg aagcattaat ttaagtacca gtatcggtgt agcgcattca
     1681 aatgaatgta ccaccgtcga acgcttgaaa atgctggcgg atgagcggct gtataagagt
     1741 aagaaaaacg gcagggctca gattagctgg cagtaatcat tattcgcagg ccggacaaat
     1801 gattttgccc ggcctgaatt aattaaaccc ggctacccca caaatcgtac tcatcggcgt
     1861 gctcgacttt cacacgcagg atatcacccg gcttaacgtt ggtttcacca ttgagataaa
     1921 ccgcgccgtc gatttccggt gcatctgcca tgctgcgacc aatcgcgcct tcttcgtcca
     1981 cttcgtcgat aatcaccaga atttcacggc ccactttctc ttgcaggcgc tcggcggaaa
     2041 tctgctgctg cagctgcatg aaacggttcc agcgttcttc tttaacttct tccggaacct
     2101 ggtcaggcag ggcattggcg tctgcacctt caaccgggct gtatttaaag cagccaacgc
     2161 gatccagacg cgcttctttc aggaagtcga gtagcatctg gaaatcttct tctgtctcgc
     2221 cagggaagcc gacaataaag gttgagcgta gggtcagttc cgggcagatt tcgcgccact
     2281 gtttgatgcg cgccagttgg cgatctacag aacccggacg cttcatcagt ttgagaatgc
     2341 gcgggctggc gtgctgcaac ggaatgtcca gatacggcag gattttgcct tctgccatca
     2401 gtgggatgac gtcgtccaca tgcggataag ggtaaacgta gtgcagacgt gtccagatcc
     2461 ccagtttcga taactgttcg cacaggctga ccatgctggt ttttaccggc tcgccgttgt
     2521 ggaagccagt acgatgttta acatcaacgc cataggcgga agtatcctgc gagatcacca
     2581 gaatctcttt aacgcccgca tctaccagac gtttcgcttc acttaacact tcgccaatcg
     2641 gacggctcac caggtcgccg cgcatagacg gaataatgca gaaggtgcag cggtgattac
     2701 agccttccga aattttcaga taggcataat gacgcggcgt cagtttcaca ccttgttctg
     2761 gcaccaggct caggaatggg ttgtgtttcg gttttggcac gtagtgatga acgtgctcca
     2821 gaacctgctc atagctatga ggcccggtga tttccagcac tttcgggtgg acttcgcgga
     2881 tctgatcttc ttttgccccc agacaaccgg tcacaattac cttgccgttt tcattcaacg
     2941 cttcaccaat ggcttccagt gattcttgta ccgcgctgtc aataaagccg caggtgttga
     3001 cgatcaccat gtccgcatcg tcatagctcg gtaccacgtc ataaccttca gtgcggagtt
     3061 cggtgagaat acgctctgaa tcgacaaggt ttttcggaca gccaagggaa acaaagccga
     3121 ttttcggctg gggagttact ttgctcatag cttaaaaaat attcagttac aggaaaggtc
     3181 agggcaggga ttctacagag ttctggataa aatttgtatc gcaatctcat tcgctggcgg
     3241 aggcgaagga aatgtaaatt ttgttaattc ggcgtgaaga attgatcctg gacagcattt
     3301 tgctcaaaaa atagccatac tatttaattg caacaaggct ggaaagagga ggatcgaagt
     3361 atgttcgttg acagacagcg aatcgatctg ctgaaccggt tgatcgacgc acgcgttgac
     3421 ctcgctgcat acgtgcaact gaggaaggca aaaggataca tgtccgtcag cgaaagcaat
     3481 catctacgag ataacttttt taaaactgaa tcgcgaactg cacgataaat cgctgcggtt
     3541 gaatcttcat ctggatcagg aagagtggag tgctcttcat catgctgaag aagcattagc
     3601 gacagccgca gtatgtttga tgagtgggca ccatgattgc ccgactgtta ttaccgtcaa
     3661 cgccgataag cttgaaaatt gtctgatgag cttaacgctg agtatccaga gcctgcagaa
     3721 gcacgccatg cttgagaagg cctgaaaact aagggggaga aagcgtctcc cccttcatgt
     3781 ttaagttttt gtaaaaatga atttgttatc tcctccactg actacgcttt aagccagagt
     3841 caatccggag gcg
//
LOCUS       DAJTNN010000250         3837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTNN010000250 DAJTNN010000000
VERSION     DAJTNN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.255"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(521..712)
                     /locus_tag="OO150_003956"
     CDS             complement(521..712)
                     /locus_tag="OO150_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5517914.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            780..1103
                     /locus_tag="OO150_003957"
     CDS             780..1103
                     /locus_tag="OO150_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5517915.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            1066..1809
                     /locus_tag="OO150_003958"
                     /pseudo
     CDS             1066..1809
                     /locus_tag="OO150_003958"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1781..2341
                     /locus_tag="OO150_003959"
     CDS             1781..2341
                     /locus_tag="OO150_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5517916.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            2348..2581
                     /locus_tag="OO150_003960"
     CDS             2348..2581
                     /locus_tag="OO150_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5517917.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
     gene            complement(3051..3518)
                     /locus_tag="OO150_003961"
     CDS             complement(3051..3518)
                     /locus_tag="OO150_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517918.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          912 a          877 c         1146 g          902 t
ORIGIN      
        1 accaggggga aacgcctggt atctttaagt cctgtcgggt ttcgccacct ctgacttgag
       61 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc caccggcgcg
      121 gcctttttcg ggctcttgtt ttttgctcac atgtttcttt tggctttatc ccctgattct
      181 gtggataact gtgttaccgt ctttgagtgc gctgataccg ctcgccgcag ccgaacgacc
      241 gagcgtagcg agtcagtgag cgaggaagcg gaagagcgcc ggaagctaca ttttctcctt
      301 acgcatctgt gcggcatttc acacccggca tggtgcactt ttcatacaat ccgcactgat
      361 gccgcataag taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
      421 cccgacaccc gctaaaacct gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      481 ctcacaggcc agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccatcat
      541 ccccgaagcg tgcgaggcag ctgcggtaaa gctcgtcagc gtggtcgtga agcgcttcgc
      601 agatgtcagc ctggtcgtcg gcgtccagtt cattcagttt ttccagcagg gtcagcgtct
      661 ggcttctgat gaagcgggcc atgttaaggg cggttttttc ctgtttagtc acagttctgt
      721 ttctccatgt gtggggagtc tgtcggaggt ttttaatgat accggtgaaa cgcgaaagga
      781 tgctcacgat acgggtcacg gatgaggaac atgcgcggct gctggcacgt tgtgagggaa
      841 aacaacttgc gtcatggatg cgtaaggtct gcctgggggc accgccatcg aaaacgtcag
      901 gtcttccgac actggcaccg ccgctgttgc gccagttcgc ttcggtcggc aataacctga
      961 atcagatagc ccggaagata aacagcggac agtggtcggg gcatgaccgg gttcatgtgg
     1021 tggctgcact gatggccatc ggacgtgaac tgagtgagct gagggatgag gtccgtaaac
     1081 agggagagcg ggatgatagt taagtttcat gccaggggaa aaggcggtgg cagtggtccg
     1141 gttgattacc tgctgggccg ggagcgtaac cgggaagggg cgagagttct gcggggtgct
     1201 cccgaagagg tgcgggaact catcgatgcc acgccgtttg cgaaaaaata cacctcgggg
     1261 gttctgtctt ttgcagagca gaccctgcca ccgggagagc gtgaaagggt gatggagagc
     1321 tttgagcggg tactgatgcc cggtcttgaa aaaaatcagt acagcatcct gtgggtggag
     1381 caccaggaca aggggcgtct tgagctgaat tttgtcatcc cgaatatgga gctggcgagc
     1441 ggaaaacggc tccagcccta ttacgaccga gcggaccgtc cgcgaatcaa tgcctggcag
     1501 acactggtga atcaccatta cgggctgcac gacccgaacg caccggagaa caggcggaca
     1561 cttgtcaccc cgaataatct gccgaaagcg aaacaggagg ccgcagaagc gattacgagg
     1621 ggactggagg ctctttacca tgccggagcg ctaaaaacgc gtcaggatgt cactgaggcg
     1681 cttacagcgg caggttttga ggttgtgagg acaaccagga gcagtatcag tatcgccgac
     1741 ccggagggcg ggcgtaatct caggctgaaa ggggcgctgt atgagcaatc ttttacaaat
     1801 ggggacagac ttcgagaaga aactgaaaga gcgtgccgtc tctacagaga acatactgaa
     1861 cagcgagttc aggaagctcg aagcgtctgt agacagggaa ctgaacttaa acgggcagaa
     1921 aatcagagac gctatcagcg cacacacagc agcggtgaaa gaacaactgg agacgctgaa
     1981 cagtgccgtg aacgcgcagt tcagcgccac agagaacgaa ctggcccggc ggcaggaaga
     2041 gctgctctgg aagctggtca aagggcggat actgtacccg tcgctgacgg cgctttgcgt
     2101 cacgggcggg atttttctgg cgtcatgggg gctgattcag tggcaggaaa gccggattgc
     2161 ggcgaacatc ctggctatca gggaccagga agagacgctg gcgaaactcc gggaaaaaac
     2221 atggggtgtg acgtatcacg agggcaggaa cgggaaattt ctggtattgc cgtccggggt
     2281 gaagggggaa aacaactgga cggtggagaa gaagaacgca gtaagactgg tcagggagta
     2341 agtgagtatg acagagctgg aaatgcactt actgaacgcc ttagagcaat tgcagcagga
     2401 ctatacgcag cggctgaaag actgggagag cgccttcacg gatttgcagc atatgttcga
     2461 gctgacacga caggagaacg ggcaactgag ggaacagtgc gacgacttga gtcagcaggt
     2521 gcagagatta gtcgagcagg tggaccactt gagtcggtta tttatcacga acagcagata
     2581 agggatgaac aggaaaggca ggcacgcctg gaacgggagc gtgcgcggtc ctggcatgaa
     2641 gagcaggaac gggaagcacg ggtatggcgt ggcccgtcaa tagggatatg acggtgagtt
     2701 gttccgggag gttgccagat ggtaccggaa cgcgttaaaa acgcatccgg gcgcgattct
     2761 ggcgtgtcgt ttttgttgct gtgttttgcc tctttgccag atcgcgctca ttcctcagcc
     2821 tcatcccgcg atgtctgcgt atgtcatcaa ctgaaaagat ggaaaaaaaa cagtgtcatg
     2881 aggtttacgc aggcaaaacc agcgttattc acatggctga atagctatgc atacggttaa
     2941 aatttatcag gtgcgatcgc ggcagctttt cgggtggttt gttgctattt ttacctgtca
     3001 gcagccgtga tcgcgctgaa cgcatttcag cggtgcgtac aattaagata ttacgttaaa
     3061 ttgtgaggga atggtcccca tatactgact ttatcatagg gatagggttg gtagtgggtc
     3121 gattgggttt cggcattgtt tccgttaatg ccaattgcat ttttgtattt gatgcaacag
     3181 aaggcgtgag attctcctgg gcctggaaag gctaagtgtt tagaattgat ggcaaaatat
     3241 cttccgtccg atttttgaag tagttgttgt ttggctgtct cccaaagggt gctgttacaa
     3301 acttcttgaa tttgtagttt ttgcagggct cttattactg cgttgtcatg ttccaattct
     3361 ttaggggagt gtattattct ctcttttaat aagctgttta ccacagggtt tagttgttgt
     3421 ctaaagcacg cagataatgc cattgctaca caattttgtt cccgaggaag catgtttggc
     3481 ggggtatgcc gtgtttgatg tgatatgtct attctcatag aaggtctctg gcttatagca
     3541 tcaacaaata cggctataaa acaaaatttt catagagtga ttcaagatct tcttgagatc
     3601 cttttttgcg gcgcgtaatc tgctgcctgc aaacaaaaaa accaccgcta ccaacggtgg
     3661 tttgtttgcc ggaatctaga gctaccaact ctttcttccg aaggtaactg gcttggtgga
     3721 gcacagatac caaatactgt ccttctagtg tagccgcagt taggccacca cttcaagaac
     3781 tcttaatatc tcaatccacc ttgtctagtt accaatggct gctgccagtg gcgttaa
//
LOCUS       DAJTNN010000251         3794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTNN010000251 DAJTNN010000000
VERSION     DAJTNN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.256"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            232..543
                     /locus_tag="OO150_003962"
     CDS             232..543
                     /locus_tag="OO150_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5517919.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            543..830
                     /locus_tag="OO150_003963"
     CDS             543..830
                     /locus_tag="OO150_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5517920.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1367..2554)
                     /locus_tag="OO150_003964"
     CDS             complement(1367..2554)
                     /locus_tag="OO150_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5517921.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(2554..2919)
                     /locus_tag="OO150_003965"
     CDS             complement(2554..2919)
                     /locus_tag="OO150_003965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517922.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            3004..3333
                     /locus_tag="OO150_003966"
                     /pseudo
     CDS             3004..3333
                     /locus_tag="OO150_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3363..3446
                     /locus_tag="OO150_003967"
                     /pseudo
     CDS             3363..3446
                     /locus_tag="OO150_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            3446..3541
                     /locus_tag="OO150_003968"
                     /pseudo
     CDS             3446..3541
                     /locus_tag="OO150_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          975 a         1000 c          886 g          933 t
ORIGIN      
        1 aaggtgaaat ctgtcaatca gtaccggctt acgccgggct tcggcggttt tactcctgtg
       61 ccatatgcaa caacggagtg ccgccttcca tgccgctaac gcggcatcaa aaaatccctc
      121 ttctccagtt gcccccctcc gaactcagag tgtattttca tttattgatg aaatttactg
      181 tttgcgcaat tttcctgttt tagtgtattt tgtcattctg gaggctataa tatgtttaat
      241 gtgattactc acccggccgc actggatgaa cttctagaat taccagacaa tctgcgtggt
      301 cgtatgacca gactgattga acggctggaa caggaaggta acaaaatgaa aatgcctcac
      361 agccgtgtaa ttggcggagg gctttttgaa ctgagagtcg gggataaaaa tatcgcaagg
      421 acactgtacg cttatgcaac gggtaacgaa atctatctgc tgcatgcatt tgtgaaaaaa
      481 acgcaaaaga caccaacgaa agctattgaa attgccagga tgcgtctgaa ggagatgaac
      541 tgatgaaagt aaaaggtgtt ccattcagcc aggtaaagca aaaactactc agtactccag
      601 aagctgtcaa agggtatgag gatgctgaca aagaactgga gatggtggaa atgttatacg
      661 aaatgagaga aaaagcggga ttaacaaagt cagcactggc cgaacgaatg gggttacgcc
      721 cgtcagccat cagtcgcctg gaaagtaacc ctttaggggc cagcatgaaa actttgacaa
      781 aatatgctca tgcctgtggc gcaaccatcg atattcacgc tgtctattga ggggtccgct
      841 tggaattcgg aaaataaacc acgttaagac tattttttag ataaatgact ttcaggaatc
      901 gccaagatgg cgggataaca gcggttttgg tgtcctaaat cgtacaattc tgtacatcca
      961 cgcaagggaa tgtacggaag taagccagac ggttatcgga cggtcagaac atttcgtaca
     1021 gcctttacgt tcagtgagtt agctggcctg gacgtaacca ctcgttagct caattctctt
     1081 agcgtgggaa aatttccgaa ttccaagcgg acccctttat ccttgccccg ctgaatgagg
     1141 aaacgctgtt ccgggggatt atgctgaacg tcttccgttc atcgatagga attaaaaccc
     1201 caaaaagatt aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctcatatg
     1261 aacggaaccg acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt
     1321 ttgctccgtt tccctcaaaa tggacgctac ttaccctctg cggctctcag ccgcaccacc
     1381 gcatccgggc cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca
     1441 gccccgtaaa acgcatctga ctcccgcaca gcacgcactt cagcgggtca accttcagta
     1501 acctctgata catccctctc caggtgattt gcatcgccgt ttttctcact gtctccgtta
     1561 tgatgtacac cacttcttcc agtaaccgcc gtttcgccgg actcagaaaa ccgtagtacc
     1621 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccatg aactcatctc
     1681 cactcatcaa caggtattct tcccgttttg ttcggtgact gttgtaacgc agtccgattt
     1741 catcctgacc ggcataatgc tccagacgac tcaccggcac cggtggcttt ttcaggtaag
     1801 agccaaagta caccgccaca tgggtggcat tatccatcac ccgggatacg ttgacattcc
     1861 agccacggcg gtaatgcgtg tccaggaagc gattccattc ccgtttactg cttccttctg
     1921 ctgccagcgc atccggcatc accaggtcag ggtatttccg tgacagtaac cgtgttatcc
     1981 ggtagcgcca catgctcatc accttacggg cgtaaaaatg aagatttttc caggtgtggc
     2041 ccgacgtcac accaccggca gttgtcgata aatggatatg cggatgccac tgctggtcac
     2101 gcccccatgt gtggatcacc gtgaatatcc tcggctccac atctgcctga tggcagattt
     2161 ccagtatcac atccgctgca atgcggctca tctctgccag caaccaccgg ttgtggaaca
     2221 ccagggacca gtactggcag ggtaacgtga acacaatatg ctgccatggg cagtcgggga
     2281 ccaggctcag cagatactgt atccactgtg cgccagcctt caccccgcag tgcgggcagg
     2341 agcggctttt acaccggaag cagacctttt ttgtatggca acagtccggt gatgaacagc
     2401 accactgtgt ataccccatc agtgtggtcc cgcacgccat gattttggtc accgactcaa
     2461 tcaccaccgg acgtactgcc ccttccggct gcttctccag ccagttaagc cagcggtttc
     2521 cctgctgaaa gatatcggca aaacggggaa gcatcagaag ggcggggcga ctccgtccgg
     2581 ccagtgaacc gtgccacact ccgggcagta cataccgccg gcgctgatac cggaaagaat
     2641 ggtcgcaaat tcccgctccg tgcagcgggc aatttccgga tacccttcgt catcaacacg
     2701 tacaaaccag aagaccagtt ttttgtttcc ggcatccaca aagaacggaa tattcaggtc
     2761 tgcgcaacac tcaacggcat catcaaaact atcaaagcgc agaacttctg cgtcttcttc
     2821 gtcaaaaaaa tcatcttcgt gaagcttcac gacatagcgg ggaagtttgc ttctttgaga
     2881 ggcgggttta cgtttacggg gtttagctga acgggccata taaccacctg aaagacaatg
     2941 acattgcctg tttttataac ggtaattgca gaccatgaca agccgcagcc gtcaggctgc
     3001 ctactcgagc ttcagctcaa taaaggcgaa tatcgacata aattaccccg atggatcttt
     3061 tttgcaaatc agggtgaatt cacgaccggt gattacgaag aaatcatgaa taaggccagc
     3121 gccctgagtc tggtatcgaa tgcaatcctg tactggaata catcaagaat aagcaatatt
     3181 gttgaagggt tacgtgaaaa tggtgaggtt attgatgatg acgtattagc ccatatatca
     3241 ttactgccct ataaacacgt attacctaat ggtacttatt ttgttgatgg agaaccttac
     3301 ggtactgagc ctaccgaaag agcaaaaccg taagtttctg ttcaaatgct cccgctggtc
     3361 catccggaca gattatcgac catcaggctg tacaggcctg ggcggacagc ctcagtactg
     3421 acaatccgtt accggtgcca cgctgatgga acagaagtgg accagtaagg cgctttctga
     3481 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaatggt
     3541 acacgtatag gaaattgaaa aaccagacga taccgctgtc cacgccttat gccacgtgag
     3601 cttttctgct tttccctcca ctccggtgtt ttctctctct gcatggtcga ttcttatacc
     3661 gcaacaggct gttttttata cttgcaccga ttcccgggca cacttagcct gtgtcaggcc
     3721 ggcatatacc tcagcagact gatatcccgc gcgtttttct tcagccctcc acattcagtc
     3781 ccgcgatggc ccgg
//
LOCUS       DAJTNN010000252         3772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTNN010000252 DAJTNN010000000
VERSION     DAJTNN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.257"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..199)
                     /locus_tag="OO150_003969"
     CDS             complement(<1..199)
                     /locus_tag="OO150_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5517923.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVE"
     gene            complement(340..2373)
                     /gene="metG"
                     /locus_tag="OO150_003970"
     CDS             complement(340..2373)
                     /gene="metG"
                     /locus_tag="OO150_003970"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5517924.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQESRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2505..3614
                     /gene="apbC"
                     /locus_tag="OO150_003971"
     CDS             2505..3614
                     /gene="apbC"
                     /locus_tag="OO150_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5517925.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHCLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          906 a          966 c          969 g          931 t
ORIGIN      
        1 gctccacata ccccttcttc accttctccg caatcagctt aagctccgct tttgccgctg
       61 ccgcagcatc tgaaaaactt tttatctggc tttgcccttt ggtgccaact tttccccaac
      121 tgatatgcaa ctcgtttccc tgttgctcaa ctgcccagaa tttatgtgat ttttcgtcct
      181 gatagataaa gtgtctcatt ctgcatccct gtaattttat ccagttgaat gcagatgcta
      241 ccagtattta tgcgggttag agagagacaa aatcgcaaga aaacaacttt aggaatttat
      301 aaagaaaagg cgctgtcgat gcagcgcctt gaagggggat tatttcacct gatgacccgg
      361 tttagcaccg gcatccgggc ttagcaggaa aatatctttc ccgccaggac cggcagccat
      421 caccatgcct tcagagatac cgaagcgcat tttacgtggt gctaggttag ccaccataat
      481 ggtgtgacga ccaatcagtg cctgcggatc cgggtaagca gaacgaatac cggagaagac
      541 attgcgtttt tcaccgccga gatccagcgt caggcgcagc agtttgtcag aaccttcaac
      601 aaactctgcg ttttcaatca gcgccacgcg caggtcaact ttagcgaagt cgtcaaaggt
      661 gatggtttcc tgaatcggat catctgccag cgggccagtt accggcgcgg cagcggcttt
      721 tacttcttct ttagaggctt ccaccagtgc ttcaacctgc ctcatatcga tgcggttata
      781 cagcgccttg aacggattca ctttgtggcc cagcagcggt tgctggatac catcccaggt
      841 cagttccgta ttgaggaatg cttctgcacg ctcggtcagt ttcggcagta ccggcttcag
      901 gtaagtcatc agcacgcgga acaggttgat gcccattgag caaattgcct gcaggtcggc
      961 atcgcggctt tcctgtttcg ccaccaccca cggagcctgt tcatcgacat agcggttagc
     1021 cagatcagcc agcgccatga tttcgcgcac ggctttacca aattcacggc tttcccacgc
     1081 ttcaccaatc acttcagcgg catcagtgaa ggttttgtac aactgcgggt cagccagttc
     1141 gcttgccagc acgccgtcaa aacgcttgtt gataaagccc gcattacggg aggccaggtt
     1201 aaccactttg ttaacgatat cggcattcac acgctgaacg aaatcttcca ggttgagatc
     1261 gatatcatcg atgcgcgaag agagtttcgc agtgtagtag taacgcaggc tgtctgcgtc
     1321 aaaatgattc agccaggtgc tggctttaat aaaggtgccg cgagacttgg acatcttcgc
     1381 gccgttcacc gtcacatagc catgaacaaa caggttggtc ggcttgcgga agttgctgcc
     1441 ttccagcatg gcaggccaga acaggctgtg gaagtaaacg atatctttac cgatgaagtg
     1501 gtacagctcg gcggtggagt ctttcttcca gtattcatcg aagcttacgc tgtcgccgcg
     1561 cttgtcgcac agattcttga aagaacccat gtagccaatc ggtgcgtcca gccagacgta
     1621 gaaatatttg cccggcgcgt tcggaatttc aaaaccgaag taaggtgcgt cgcgggagat
     1681 atcccactgt tgcaggccag attcaaacca ctcctgcatt ttgtttgcca cctgctcctg
     1741 caacgcaccg ctgcgggtcc atgcctgcaa catttcgctg aaagagggca gatcaaagaa
     1801 gaagtgttca gaatcacgca ttaccggggt agcgccagaa accaccgatt tcggctcgat
     1861 cagttcagtc gggctgtagg tcgcgccgca gacttcgcag ttatcgccgt attgatccgg
     1921 ggatttacat ttcgggcagg tgcctttcac aaaacggtcc ggcaggaaca tgcctttttc
     1981 cggatcgtac agctgagaga tggtgcggtt tttaataaaa ccgttttctt tcaggcgaga
     2041 gtagataagt tctgacaact ggcggttctc ttcgctgtgt gtcgagtgat agttgtcata
     2101 gctgatgtta aagcctgcga aatcagtctg atgctcctga ctcatttcgc caatcatctg
     2161 ctccggggtg ataccaagct gctgagcttt cagcatgatc ggtgtaccgt gggcatcgtc
     2221 ggcgcagatg aagttgacct cgtggccgcg cattcgctgg taacggaccc agacatcagc
     2281 ctggatgtgc tccagcatat ggccgaggtg gattgagccg ttagcgtacg gcagtgcgca
     2341 cgtcaccaga attttcttcg cgacttgagt catagtaggc attacttctt aatagtgaaa
     2401 aggggttaga tagtaccaaa tgggaaaatg ttaagtaagt acttctctat attcttcagg
     2461 taaactatca gctgatggtt tatctataca aaaaaggagt cgggatgaac gaacaatccc
     2521 aggccaaatc accagaagcc ttgcgcgcaa tggtcgccgg gacactggcc aattttcagc
     2581 acccaaccct gaagcataac ctcaccacgc tgaaagcgtt gcaccatgtc gcctggatgg
     2641 acgacacgtt gcatgtggaa ctggttatgc cattcgtctg gcatagcgcg tttgaagagt
     2701 taaaagagca atgtagcgcc gaactgctgc gtatcaccgg ggcgaaagct atcgactgga
     2761 aactgtcgca caatatcgcc accttaaaac gcgtgaagaa tcagccgggt attaatggtg
     2821 tgaagaacat cattgcggtc agttccggta aaggcggcgt gggtaaatcc tccacggcgg
     2881 taaacctggc gctggcactg gcggcggaag gggcgaaagt cggcattctg gacgccgata
     2941 tctacggccc atcaattccc accatgctgg gcgcagaaaa tcagcgtcca acttcgccgg
     3001 acggtaccca tatggcaccg attatgtctc attgcctggc aaccaattca attggttatc
     3061 tggtcactga cgacaatgcg atggtgtggc gtggaccgat ggcgagcaag gcgttaatgc
     3121 agatgttgca ggaaaccttg tggccggatc tcgactatct ggtgcttgat atgccgccag
     3181 gcaccggtga cattcaattg acgctggcgc aaaacattcc agtaactggt gcagtcgtgg
     3241 tcaccacccc gcaggacatc gcgttgatcg atgcgaaaaa aggcattgtg atgttcgaga
     3301 aagtcgaggt tccggtgctt ggtattgtcg agaacatgag tgtgcatatt tgcagtaact
     3361 gtggtcacca tgaaccgatt ttcggtaccg gtggtgcaga gaaactggct gaaaaatacc
     3421 acacacagct gctggggcag atgccgttac acatctcctt acgtgaggat ctcgataaag
     3481 gcacgccaac ggttatcagc cgtccggaga gcgaatttac cgctatctac cgccagctgg
     3541 ctgaccgcgt tgcagctcag ctctactggc agggtgaagt cattccaggc gagatttcct
     3601 tccgcgcggt ctaacgccta ttaaccattc tggttattct tcatacaccg cagagatgtt
     3661 acattgatgc ggtgtattag tttttgccgc attaatgaac acttatttaa tactgcgaat
     3721 attctgcgga ccttcgtccg aaactctaca aagtcgattg atcatacagg ca
//
LOCUS       DAJTNN010000253         3770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTNN010000253 DAJTNN010000000
VERSION     DAJTNN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.258"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..2299
                     /gene="torA"
                     /locus_tag="OO150_003972"
                     /pseudo
     CDS             1..2299
                     /gene="torA"
                     /locus_tag="OO150_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            2296..2895
                     /gene="torD"
                     /locus_tag="OO150_003973"
     CDS             2296..2895
                     /gene="torD"
                     /locus_tag="OO150_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5517926.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(2988..3293)
                     /gene="cbpM"
                     /locus_tag="OO150_003974"
     CDS             complement(2988..3293)
                     /gene="cbpM"
                     /locus_tag="OO150_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5517927.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(3293..>3770)
                     /locus_tag="OO150_003975"
     CDS             complement(3293..>3770)
                     /locus_tag="OO150_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="functional analog of DnaJ; co-chaperone with DnaK,
                     molecular chaperone in an adaptive response to
                     environmental stresses other than heat shock; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5517928.1"
                     /translation="FGMIEQEIPKTLNVKIPAGVGNGQRIRLKGQGTPGENGGPNGDL
                     WLVIHIAPHPLFDIVGQDLEIVVPVSPWEAALGAKVTVPTLKESILLTIPPGSQAGQR
                     LRVKGKGLVSKKQTGDLYAVLKIVMPPKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT          867 a          987 c         1070 g          846 t
ORIGIN      
        1 ggattgccgg attgccggat tgccggatca cgtacacaac gcggcgcgta ttcgttatcc
       61 gatggtacgc gtggactggc tgcgtaagcg ccatctcagc gatacctccc agcgcggtga
      121 taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga
      181 acgcgtgcag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac
      241 ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag
      301 cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt
      361 cggttcgatg gaagtgtatg aacagcaaac ctcctggctg ctggtattgc agaacagcaa
      421 aaccattatg ctgtggggct ccgatttgct gaaaaaccag caagcgaact ggtggtgccc
      481 ggatcacgat gtttatgaat attacgagca actaaaagcg aaagtcgccg ccggtgaaat
      541 tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca
      601 tgtgaagcac attgcggtta acccgcaaac tgacgtgccg ctgcaactgg cgctggcgta
      661 tacgctgtac agtgaaaacc tgtacgacaa aaactttctc gctaactact gtgtaggttt
      721 tgagcagttc ctgccgtatc tgctgggtga gaaagacggt cagccgaaag atgccgcatg
      781 ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt gggctggcgc ggcagatggc
      841 ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca
      901 gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg
      961 tggttttggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
     1021 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
     1081 aggctacagc agcaccattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
     1141 agtgatcaac tggaacggta aatcggtaaa actgccgcca ctgaaaatgt gtatttttgc
     1201 cggaactaac ccattccatc gccatcagca gatcaaccgc attatttaag gctggcgcaa
     1261 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
     1321 cgtactgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
     1381 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg cagttcgagg cgcgcaacga
     1441 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgc gaagaagcct ttaccgaagg
     1501 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
     1561 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
     1621 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
     1681 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
     1741 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
     1801 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
     1861 gcagttatgt gagtcggaaa ctctgcgtca gcaatatacg gtagcgggta aagagccagt
     1921 attcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
     1981 ctttaacgct cgcggtcagg tgttggcagg ggcagtagtt tctgaccgct atgcacccgg
     2041 cgtggcacga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
     2101 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcggtactt cgcagctggc
     2161 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gagcagtgga
     2221 gcaggtgacg gcgtttaacg gccctgtgga gatagtggcg cagtgcgaat atgttcccgc
     2281 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
     2341 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
     2401 cagatggcgg aatggttttc gttgctgaaa agcgaaccgc cgctggctgc ggcggtgaat
     2461 gagctggaaa actgtattgc cacgctgaca gtacgtgacg atgcccgtct ggaactggcc
     2521 gctgactttt gcggcctgtt tttgatgact gataagcagg cggcgctgcc gtatgcatcg
     2581 gcctacaaac aggacgagca agagattaaa cgcttgttag ttgaggccgg gatggaaacc
     2641 agcggcaatt tcaacgaacc ggcagaccat ctggcgatct atctcgagtt gttgagtcat
     2701 ctgcattttt cgctgggaga ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa
     2761 aaaacactga cggcgctgtg gcaatggtta ccagagtttg ctgcgcgttg ccatcagtat
     2821 gacagctttg gtttttacgc ggcactaagc cagttattgc tggtgttagt ggagtgcgac
     2881 caccaaaaca aataacgtcg tttatgcgcc tgaaaagacg cgtttagcgt cgcatcaggc
     2941 attatggcgc agttgccgga tgcgtcatca catccggcaa acacacatca cggatgagct
     3001 acaaaccggg aaagccgctg gcgtagcagg cggttttcct gcttcaggtg tgcaatatca
     3061 tccattaacg tcagcgccac cgcgatcccc ggccagtcca gagccagttc atgacgcagg
     3121 cgtaccgcgc gttgcaccac aatggcggca tggtcgtcaa atacccaggt tgtttcctgg
     3181 atctcacgcg gttcaaccac ccccaaaccg acaatttcat tcaactcctc ttcagagatg
     3241 ccggtatgca ggcaaaattc ggtaatagta aaagtcaccg taacattagc catcatgctt
     3301 tcccccaatc tttacgtgga tcaaaagacg actgggcgtc tgccagttgc tgccacagcg
     3361 cggcagtgtt ttcatccggt ttcggcggca tcacgatttt cagtaccgca tacagatcgc
     3421 cggtctgttt tttgctcacc agacctttgc ctttaacgcg caatcgttgc ccggcctggc
     3481 tgcctggcgg gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg actttagcac
     3541 ccagcgccgc ttcccacggg ctaaccggca ccacaatttc cagatcctgg ccgacaatat
     3601 cgaacagcgg atgtggcgca atatgaatca ccagccacaa atcgccattt ggaccaccgt
     3661 tttcgcccgg cgtcccctgg cctttcagac ggatgcgttg accattgccg acgcccgccg
     3721 ggatcttcac attcagcgtt ttcgggattt cctgttcgat catgccaaag
//
LOCUS       DAJTNN010000254         3752 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTNN010000254 DAJTNN010000000
VERSION     DAJTNN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.259"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            315..3434
                     /locus_tag="OO150_003976"
     CDS             315..3434
                     /locus_tag="OO150_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5517929.1"
                     /translation="MIGQFLSATEILAKNYVRNKMVKNPFYSNLKWNFIEKNIIRLTS
                     SPVKSVLCISAFSFVLLYVGYLNELFIKNNLLHYFPFRHSLTEWQTTILSGQLTIIGI
                     VYPLVIGLVSVLFQKKADRKIAQTAYQRYSGFMLAGLSGLFLSGFILLSVLIKTVFGS
                     YLYGIACLISILWLLINIVLSIWFFIVSLEILDDVKRQIIIKRYIAFEIVMPHICNKI
                     SAKLRLYPIYQKHNYSNLEITQADYKGEYISVASSYSKEDELSLYHRPFQLTLNLINY
                     QLKKKNHFASFVIGDNRTKETESTGKILFSVKNIKPDSLLIKILKQCFYRAPIKGGDF
                     SVSLTMQAITADTYMYLRDSDLISFDNAISALINNFNNLCDLYFFQDDNTNNNFLLIT
                     TELFERSFQYEFSDEVYKISNNSMDKINLSERFFELCLWSGVRIINNRKHLISNELCI
                     YMGITRSQWSILTEWFRNNQSLLNASLRSRYNRILRTYITVWEQYQESINFRFCNTEN
                     SDLFELFCKTQLQELPSMIIDATQTRDPSTIDTAVDLINRWQHSMNIDSHSVEKYSYK
                     GQLFNPGFFISKKLNFNSDREWFNIAIINALTDMRICTCLYLTSRINTSDKLMTHYIK
                     LILEGKLIDQTGGYETPTEEIDNASQLIKILIRICLWTWSENMEHNGWMNSLARRLRD
                     YDKTDMVMGRVYSNVFDCGFIDMEQSWVQLLLIFSNKNDSVSKEIKEAIENNYITYRE
                     KQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKENLRKLLQEHINMLKKDLDMRLQDA
                     AIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSIDFKQASDCFTKHKISIKIDKEPYA
                     EGIESIPYINEGDIQADLILKDIQRIILSNLFSTGCSQHTVIEDFNMLIDHIKSSADL
                     AGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKNDDGSMNITTESGTRKVYFLPFVNQP
                     FSLVVKDNYFTKLIIREYDNNKLVNVTSENIKSDSDKFKLTLNYELNIVFEGNADLKI
                     AHSQRVTSE"
     gene            3570..>3752
                     /locus_tag="OO150_003977"
     CDS             3570..>3752
                     /locus_tag="OO150_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5517930.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASPIHSS"
BASE COUNT         1318 a          658 c          620 g         1156 t
ORIGIN      
        1 agcggcttta ccacgcagct tgaacttgaa gcaaaaatcc cggaatggat agcagaaaca
       61 gaataaatct cttaaaaata ttatttttga gatgtgcttc tacactgaca gtaagaacaa
      121 aagataatgt atcagcccac tagatgtcag aggttggggc atcaaccaga tgctaacaat
      181 aatacggatg tttttcccac atgacagagg aagtcaagta taatcttcac agtccatccc
      241 gtccaaggga atatgactca atagtaaaag ttccagttta gttagtagac ccacacttta
      301 tagaagagta attcatgatt ggacaatttt taagtgcaac agagatttta gcaaagaact
      361 atgttcgtaa taaaatggtt aaaaatccat tttattcgaa tttaaaatgg aattttattg
      421 aaaaaaacat cataagatta acatcctcac cggttaaatc agtactttgc atatcagctt
      481 tttcatttgt actactttat gttggatatt taaacgaatt atttataaaa aataatcttt
      541 tacattattt cccgtttagg cattcattaa ccgaatggca aacaaccatt ttaagtggcc
      601 aattgacaat tattggtatt gtataccccc ttgttatagg tttagtaagt gtcttattcc
      661 aaaaaaaggc agatagaaaa atagcccaaa ctgcttatca gcgttactca ggattcatgc
      721 ttgctggact cagcgggctt ttcttatcag gatttatact tctcagtgta ctaattaaaa
      781 ctgtctttgg aagttatctt tatggtatag cttgtctaat cagcatatta tggcttttga
      841 taaacatagt tctttctatt tggttcttta tcgtgagtct tgaaattctt gatgatgtaa
      901 aacggcaaat aattataaag cgatacattg ccttcgaaat agtaatgccc catatttgta
      961 acaaaatatc agccaagctt aggttatacc ctatctatca aaaacataac tattcaaatt
     1021 tagaaatcac gcaagccgat tacaaaggag aatacatatc tgtggctagc agttattcta
     1081 aggaagatga gttaagttta tatcaccgtc cttttcaact tactcttaat cttattaatt
     1141 accaactaaa aaagaagaat cattttgcgt catttgtcat tggtgataat cgtacaaaag
     1201 aaacagaatc cacgggtaag atacttttta gcgttaaaaa tatcaaacca gacagcttat
     1261 taataaaaat acttaagcaa tgtttttata gggctccgat taaaggagga gatttttccg
     1321 taagtcttac aatgcaggct ataacagcag atacgtatat gtatctgcgg gattctgatc
     1381 tcattagttt tgacaatgct atctctgcat taattaataa ctttaataat ctctgcgatt
     1441 tgtatttttt tcaagatgat aataccaaca ataacttctt actgatcact acagaattat
     1501 ttgaacggag ttttcaatat gagttttcag atgaagttta taaaatatca aataattcca
     1561 tggataaaat taatctttct gaaagatttt ttgaattatg tctttggagc ggagtgcgta
     1621 ttatcaataa tagaaaacat cttatcagca atgaactttg tatctatatg ggaattactc
     1681 gttcacaatg gtcaatatta acagaatggt tccgaaacaa ccaatcactc ttaaatgcct
     1741 cgcttcgttc acgttataat cgtatattaa gaacctatat cactgtttgg gagcaatatc
     1801 aagaaagcat aaactttcgt ttctgcaata cagagaattc agatttattt gaattatttt
     1861 gcaaaacgca actacaagaa cttccatcga tgattattga tgcaacacag actcgcgatc
     1921 cttctactat tgatacagcc gtcgatctta ttaaccgatg gcagcactca atgaatattg
     1981 acagccattc ggtagaaaaa tacagttaca aaggccagct ttttaacccg ggttttttca
     2041 tatccaaaaa actaaacttc aattcagaca gagagtggtt caatattgcc atcataaatg
     2101 cgttgactga catgcgtata tgcacatgtc tttatctgac atcaagaatc aatacttctg
     2161 acaaactaat gacacattac attaaactta ttttagaggg taaacttatt gaccaaaccg
     2221 gtggatatga aactcccact gaagaaattg ataatgcaag ccaactaatc aagatattaa
     2281 ttagaatctg cctgtggaca tggtctgaaa atatggagca caatgggtgg atgaatagtt
     2341 tagcaaggcg tttacgtgac tatgataaaa cagacatggt gatgggcaga gtttacagta
     2401 atgtttttga ctgtggattt attgatatgg aacaatcatg ggttcagcta ttattaattt
     2461 tttctaacaa gaacgatagc gtatcaaaag aaataaaaga agcaatagaa aacaactata
     2521 taacatatcg tgagaagcaa cgactcattg gagtattatc aaaaatttgt aactccatcg
     2581 aatacactaa aatcaagctg actttaacat tggacgatct ccaaacaaaa aaagaaaact
     2641 taagaaagtt gcttcaagag catattaaca tgctaaagaa agatcttgat atgagacttc
     2701 aggatgctgc catagatgta catcgactag atagcactgc acgtaaaaca tcagaacatc
     2761 ttcgtaaacg catcaagaaa acgctccctc tttcactttt caaatctatt gattttaagc
     2821 aggctagcga ttgtttcaca aaacataaga tatctattaa gatagataaa gaaccgtatg
     2881 ccgaaggaat agaatccatc ccttatatca atgaaggtga tattcaagca gacttaattc
     2941 tcaaggacat ccagagaata atactttcaa atttatttag cactggctgt tcgcaacata
     3001 ccgtaataga agatttcaat atgttgattg atcacataaa atcatctgct gacttggctg
     3061 gcaaactggt tcttgtgatg agtaaagaga tctttcagca atataatcgt atgcttttcg
     3121 ataatcccaa cctcagagaa ctaatgagaa aaaatgatga tggctctatg aacataacta
     3181 ctgaatccgg tacccgtaag gtttacttct taccatttgt taatcaacca ttttctcttg
     3241 tggtaaagga taactacttc actaaattaa ttattagaga atatgataat aataagttag
     3301 taaatgtcac ttcagagaat atcaaaagtg actcagataa attcaagcta actcttaact
     3361 atgagttgaa tatagtattt gaaggaaacg ctgatttaaa aatagctcac tcgcaaagag
     3421 tgacatcaga ataatatctt tgacggcgct ccttagagtg ccgttcgttt attaactaat
     3481 gctaaaaatc attatataga gaaagtcatg tttaaatctt agagaataca acctagaata
     3541 gtgtaagcac tacaataaga gaggtcgcta tgttccgttg tccgctttgt ggcgcatctg
     3601 cccgtatccg caccagtcgt ccggaaaatg attcaaacac cgtgagacaa aagtattatc
     3661 agtgtaacaa tctagaatgc ggcgtatgtt tctcaacact ggaagccttc cacaaattca
     3721 cttcaaagca cgcctccccc attcactcat ca
//
LOCUS       DAJTNN010000255         3738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTNN010000255 DAJTNN010000000
VERSION     DAJTNN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.260"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..861)
                     /locus_tag="OO150_003978"
     CDS             complement(<1..861)
                     /locus_tag="OO150_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5517931.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEI"
     gene            complement(886..1764)
                     /gene="yihU"
                     /locus_tag="OO150_003979"
     CDS             complement(886..1764)
                     /gene="yihU"
                     /locus_tag="OO150_003979"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5517932.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTQAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGTPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1937..2833
                     /locus_tag="OO150_003980"
     CDS             1937..2833
                     /locus_tag="OO150_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5517933.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYNLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            2873..3661
                     /locus_tag="OO150_003981"
     CDS             2873..3661
                     /locus_tag="OO150_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5517934.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDLNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
BASE COUNT          963 a          955 c          902 g          918 t
ORIGIN      
        1 aatttctgcc aaccgttgta aacgaggaac agagacatcg cgcagcatag tttgtggatc
       61 ttgtgcacca acaaccgacg cccaaaccgc tcgtccagcc aggaagcccg acgcaccacc
      121 tttcatggca atacttactg cacggccaaa aatatcggca tcaacgccag aagaaagaat
      181 tacccacggc atcttcatct gatcgttaag ttgctgacag gcggcaagta aggttttttc
      241 atcgcctttg ccccctaatg gcatctcggc tttgtaaaga tcagcctctg ttccaccaag
      301 ctctgcggcg gcggcaacaa tggcgctttc acgatcaaag tcccaaccgc gacgaggtgg
      361 acgcaccaca ggttcaataa tgctcaccag cccggcacta cggcagcgac gaacaaattt
      421 atcaaccatt gccagacgtt cctctgcggg ttcatcttca cgccagagta caagtaattt
      481 cattgcctta gcccccattt cacgggcttt atgcgggtcg acggacatat ctatttcaac
      541 gctatcaacc gggatgccat tacctgggat aaaaagatcg gcagcgacaa tcaggccgca
      601 agaattagca acagcgccct gctcgactat ttgctcaaga caaaattgtt tatccgccag
      661 aacggcagaa gcataaggag aaaggattcg ggtcgcggca actttaaaat cggtgagtac
      721 actgtcggcg atgggttttg gctgccctgc tgccgcaaac atcaagcgca tggcctcacg
      781 ctgatcgaca gctaacatgg caaatccacc acctgggcgg gagatatctt tcagcgtaaa
      841 tggggtgaag tgggatgaca tagaaactcc tgttaataga taaaattaaa gaccagcctg
      901 ctggcgaacc tgatttagca gggcactcca gtcctgccgc cctttgccgt cttgacttgc
      961 tgcagcgtaa taactttctg ctgcaatccc catattgagc ggtgtccccg tctcccgagc
     1021 gacgtcaact gcaatacgca gatctttcaa cgccagatcc accatgaagg cgggtgaaag
     1081 atcgcctttc agcactttgc caggccaggt ggtcgtaaaa tgtcctttcc ctgctgccgt
     1141 accactcatc accttaattg cgacatcaag atttaatccc agtgactcac ataacacagc
     1201 ggcctcggag gacagcgcat taagcgcaat gctcatatag ttgttaatta gcttcacgcg
     1261 gatccccatt cctgggccgc cagcctcgac cagctcatta cccatgcaca taagaatcgg
     1321 gcgtgcacgt gcaatctgct catcagtgcc acccgctaaa atcagcaatg tgccatcaat
     1381 cgcattcacg gacgttctgc ccacgggagc gtccatcata ctgatgcctt tttcttgtaa
     1441 ctcccgtatt aatgcatcgg tctgtaaggg atgaatagtc gacatatcaa tcaataacgc
     1501 gtcagatgaa acagcctcac agacgccttt ttcccccaac agaacctgtc gaacaatgtc
     1561 accattggga agcatagtga tgataaattc agcgcctgct gctgcttccg cttgggtttg
     1621 tgccgcagtg gccccctgcg taactagcgc atcgacggcc tgtgcattga catcaaatac
     1681 ctgcaatgaa tgcccttttt gcaatagatt gttagccatt gggctaccca tctgccccag
     1741 cccgagaaat gcaatggttg ccatgaatac tctcctgtct gacattgaca aaataatgac
     1801 aattgaaaat tcgtttttgt ctgtttttga gcatatttgt gatttatgtt caaaaacacg
     1861 acacgcatca ctaatttgat catttgcgtc atttaaattg accaaagacg acaaaattaa
     1921 acaggacaag acaaatatga ccagaattgc atgcgtgggt atcacagttc aggacagaat
     1981 ttattctttg cccactctgc cggagggcgg cgggaaatat caggcaaatc attatcttga
     2041 gattggtggc ggcccggcag caacagccgc tgttgcaatc gcaaagcttg gcgtggaggt
     2101 tgatttcatc ggtcgcgtcg gcgatgacag ttgtggaaat accttacttg cagaacttga
     2161 gggttggggc gtcaataccg cattttgccg ccgatatcct aacgcccgtt cgtcgcaatc
     2221 agcgatcctc gttgatcagc atggcgaacg gattatcgtt aattatccaa gtcctgatct
     2281 cggtactgat gccgaatggt tagaagcgat cgatttctct cgttataatc tgattctggc
     2341 cgatgttcgc tggcatagcg gtactgaaaa agcattctcg ctagcgcgcc ttgcgggtgt
     2401 gacaacgctt ttagacgccg atatgacacc gcaggatatt tctccacttg tcgcgcttgc
     2461 cgatcacgcc gtgttctcaa caccagggct gaaacgcatg actggcctgc agtctcctga
     2521 agaaggtttg ttccaggcaa cgacacaaac agcaggtaaa gtgtatgtta cgctgggttc
     2581 tgaaggatcg ttgtggattg aagatggtca tttgtgtcaa caggaggcat tttctgtcaa
     2641 tgtggttgat accactggag caggcgatgt ttttcacggc gcactcgcgg tagcgttggc
     2701 agaaaaaatg ccaacaaaag aggctattcg ttttgccagt gcggttgccg cgatgaaatg
     2761 tacccagcct ggcggtcggg ccggcattcc taatcgtgag caaaccgaat catttttgtc
     2821 actttatgcg taaaatggta aacatctacc gaggaggggc aagggagtat tcatgagtat
     2881 catcgaagtg acaggtaatc cacgccatga ccaactcgtt catctaatcg cagaacgcgg
     2941 ttatatgaat attgaagaac tggcacaact gctggatgtt tcaacgcaaa ccgtgcgccg
     3001 ggatatccga aaactcagtg agcagggctt gatcacccgt catcatggag gggccgggcg
     3061 cgtctccagc gtcatgaata ccgcttttga gcaacgggaa ctttcgctca ccgccgagaa
     3121 acgggcgatc gctgaggcag tcgccgatta ccttcccgaa cgctgtaccg tctttatcac
     3181 catcggtaca accgtagaag ccgttgccag ggcattactc aaccggcgtg atttacgcat
     3241 tatcactaac agcctacgtg tggcacagat tctttataag aatcaggata ttgaagtgat
     3301 ggtgccggga ggcactttac gcgctcataa cggcgggatt atcggcccag gagccgtgga
     3361 ttttattgaa ggcttccgcg cagattattt aatcaccagt ataggtgcca ttgaacatga
     3421 cggcacccta ctggaatttg atctaaatga agcgttagtc gcgagaacga tgattaaaca
     3481 tgcgcggaat acattgttag tggccgatca tacgaagttt gccgcgtctg ctgccgtttc
     3541 aattggcaat gcacggaatg tcagggcttt ttttactgat gccccgcctc ccaattcttt
     3601 ctgccagttg ttaagtgaag agaatgttga actggtggtt gccgagcaag aagtatcctg
     3661 aaattgaaat ttcggaaaat ccgctgggaa tttcagcgga ttttgttttc cataagcaaa
     3721 aaaaacctgc cagcgatg
//
LOCUS       DAJTNN010000256         3658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTNN010000256 DAJTNN010000000
VERSION     DAJTNN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.261"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1189)
                     /gene="gcl"
                     /locus_tag="OO150_003982"
     CDS             complement(<1..1189)
                     /gene="gcl"
                     /locus_tag="OO150_003982"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5517935.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKTVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTYFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLS"
     gene            complement(1279..2094)
                     /gene="allR"
                     /locus_tag="OO150_003983"
     CDS             complement(1279..2094)
                     /gene="allR"
                     /locus_tag="OO150_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5517936.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(2172..2654)
                     /gene="allA"
                     /locus_tag="OO150_003984"
     CDS             complement(2172..2654)
                     /gene="allA"
                     /locus_tag="OO150_003984"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5517937.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            2884..>3658
                     /gene="allS"
                     /locus_tag="OO150_003985"
     CDS             2884..>3658
                     /gene="allS"
                     /locus_tag="OO150_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5517938.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSR"
BASE COUNT          925 a          990 c          886 g          857 t
ORIGIN      
        1 gtgacagacc gatggtggtg acataacaaa catcgcgacc aaaggctttg ttcatctctt
       61 catacacgcg ctgcggtttc accggcacgt tgtcgaagta ggttttgcgc agcaaagtgc
      121 gtttgcgctg ctggcagtcg gcgacccatt ctttacgaca cggcagacga cccgcttttt
      181 gcatctcctg cgccacttca accagcagtg tcagcgccgc tttagcatca gagacaatgc
      241 cgagatccgg acacagcacg cgaccaattt gcgtcggctc aatatcgata tgaacgattt
      301 tgcgcccttc ggtgtatttc tctaccgaac cagtatgacg gttagcaaaa cggttaccga
      361 taccaaacac catgtcggac gccagcaacg ttgcgttacc gtaacgatgc gcggtttgca
      421 gaccgaccat cccggccatc agttcatgat cgtccgggat acagccccag cccatcagcg
      481 ttgggatcac cggaacgctg gtcagttcag caaactgttg taacagtgca gctgcgtcag
      541 cattgattac cccgcccccg gcaacaatca ccggacgttc ggcctggatt aacatttcta
      601 cagttttttc gatctgcata cggctggcag caggtttgta gaccggcagc ggttcgtaca
      661 tgtcaggatc aaactcgatt tccgcaacct gaacgtcgaa cggtaaatcc accagtaccg
      721 gacccggacg accagaacgc atcaggtgaa atgcctgttg cagcacgcga ggcaccagcg
      781 ccgcttcacg aactgtaacc gccattttgc tgaccggttt agcaattgct tcaatatcta
      841 cggcctgaaa atcttcttta tgcagacggg cgcgcggtgc ctggccggta atgcacaaaa
      901 taggaatgga atcagcagaa gcggaataga gcgcggtgat catgtccgtg cccgcaggac
      961 cggaagtccc cagacatacg ccgatattcc ctgccgttgc gcgggtataa ccttccgcca
     1021 tgtgcgaagc accttccaca tgacgcgcca gaatgtgacg aataccgccg tgcttacgca
     1081 tcgctgagta gaacggattg attgcagctc ccggaacacc gaaggcggtg gtgatacctt
     1141 ctttctccag cacatacatt gccgcgtcaa cggctctcat ttttgccatt ttattcctat
     1201 ctctatttat tggaaaattt ttccaacttt caaattttgt caaaaaaacc cgctccgcag
     1261 agcgggatgc gacagacatt atggatgtgc tttcagtccc aacgccgtgc tgatatcgcg
     1321 ggcggtgtct ctgaccagct caccctgact gacaaaacga tcttctgtca gtcttgatga
     1381 aggcccggag atggagatag cggcaacaac gctaccgaca tcatcgtaaa ttgctgaagc
     1441 tatgcaattc agacctacaa catgctcttc tttatctacg gtatagccca gttcacgcgc
     1501 ttgttccagg tccttcagca aggtgggcat atccacaagc gtagttggcg taaactgctg
     1561 caaaccggtt tgcagaatga tgctcatcaa ctcctcttcc gccagcggat aaagcagcgc
     1621 tttgcccgca ccggaagcat gcagcggcag acgactgccc agtggcgcac acatcctgac
     1681 cattgattta cactctaact gaccaattaa tactgcttca ttgccgttac ggatcgcgac
     1741 attgaccgtt tcgccggaaa gtaacatcag gcggcgcata aacggcccgg cgacggagag
     1801 gacatcgcgg ttatggatgt acgccgcacc gacgttaaag acacctaatc ctatatgcca
     1861 ccagcctaat tgactgtcct gatagacaaa atccgctgct tgtaaaacct tcagcaagcg
     1921 aaaggtcgtg gagagcggca aatccagatt gagagaaata tcgctaaccg acgaacttcc
     1981 cccacttttt tccagatatt gcagaatcgc aattccccgc tctaacgcct gtgcgccctt
     2041 ctgtgccaca ggctccgcct gtcctggcct gccgcgccgt ctaacttccg tcatacctac
     2101 gttaactccg acctacaagt gagcataaac aatgaacaat tagtttatct tatgcaagtc
     2161 ggttgcaggc gtcacgcaaa acagagttcc tgttcaggaa tactttcaac atcacagttg
     2221 tcactgccgc cgcgatcgat ggtcagaaaa tcggtgacgc gctgccaggc gaaaagtggg
     2281 tgatgccaga cgttacgatg gtaattcact ccctgttcgc cgttggtgat aaacgcccgc
     2341 agcgttgaca ggtctggttt gtcgtcgcct aacgccacga ccaccacaaa cacttcacct
     2401 ttcatcggga taaaggcctg agtgcccagc ggatgacgtt cgagttcgtg aatggtcagc
     2461 ggcagattcg ccggttgcgc gcggttaatg ctaataagcg tacggtcttg ctcaagaatc
     2521 tcaaccagcg ccaaatcgtg gtaacgctcc accaggccat tgttaatatg gaaaaaatcc
     2581 cgttgctgag tttcgattac gtcgccataa gcactaaagg cttcctgact taacggtaat
     2641 acctgaagtt tcatcttttt gtccttgtaa cataattccg ttcaaccacc gccgaccggt
     2701 cagcttgggt tcaataacag gcaatctaaa accttaagag aacaacatca atacaaaagt
     2761 tggaatattt ttccaaaact agatttagtc cacaaattga acagggttat catgtaaaaa
     2821 tactgataaa tcagaggata agaaaatcac gcaacttgcg gatttaaaag gggatacctg
     2881 aagatgttcg atccagaaac cttgcggact ttcattgcgg ttgctgaaac aggaagtttt
     2941 tcaaaagcgg cagaacgatt atgtaaaacc acggcgacga tcagttatcg cattaaactt
     3001 ctggaagaga ataccggagt agcgctgttt ttccgtacga ctcgtagcgt aacgttgaca
     3061 gcggctggcg agcatctact ttcccaggcc agagactggc tgagctggct ggaaagtatg
     3121 cccagcgagc tgcaacaggt gaatgatggc gtggaacgcc aggtgaatat tgtcatcaac
     3181 aacctgctct ataaccccca ggccgtcgcc cagttgctgg cgtggctgaa tgaacgttac
     3241 ccctttaccc agtttcacat ctcccgacaa atctatatgg gcgtctggga ctcgctattg
     3301 tacgaaggtt tttcgctggc tatcggcgtc acgggaactg aggcgctggc aaataccttt
     3361 agtcttgatc ccttaggatc ggtgcaatgg cgatttgtca tggcggcgga tcatccgctg
     3421 gcgaacgttg aagagccgct aacagaagcg cagttgcgtc gctttccggc ggtcaatatt
     3481 gaagacagcg cccgcacctt aaccaaacgc gtcgcctggc gattgccagg gcaaaaagag
     3541 attattgttc ctgatatgga aacgaaaatc gccgcccatc tggcgggcgt tggcattggt
     3601 tttttgccaa aatcgctttg ccagtcaatg atcgataatc aacaactggt cagccggg
//
LOCUS       DAJTNN010000257         3560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTNN010000257 DAJTNN010000000
VERSION     DAJTNN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.262"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1673)
                     /locus_tag="OO150_003986"
     CDS             complement(<1..1673)
                     /locus_tag="OO150_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5517939.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRVLQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSV"
     gene            complement(1734..2366)
                     /locus_tag="OO150_003987"
     CDS             complement(1734..2366)
                     /locus_tag="OO150_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5517940.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TCFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(2303..3046)
                     /locus_tag="OO150_003988"
     CDS             complement(2303..3046)
                     /locus_tag="OO150_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5517941.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            complement(3052..>3560)
                     /locus_tag="OO150_003989"
     CDS             complement(3052..>3560)
                     /locus_tag="OO150_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5517942.1"
                     /translation="GFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGTVVRRKV
                     YAHFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGR
                     IMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGF
                     LSINKLSQ"
BASE COUNT          731 a         1152 c          923 g          754 t
ORIGIN      
        1 acgctgtatc cggcaacgcc gtcaggaaca cggctcactt ttaccttcac gccgtcggtg
       61 acggactgga cctccacgct gaccggattg ccacttccgt caaccaggct tatcagcgtg
      121 gtaccggagg atggcagcgt gatttcacgg tcgagcgtca gcgtccgggt ctggctgttc
      181 accgccagca cgcgcccgcc gatgctgatc cccgcatagt catcatcgca gatttcaata
      241 acatcacccg gcacatggcg aagcccttct gcgccgacgc tgaaatccac ggtctgcgtc
      301 tccagcagct ccgttttaat cagccacagc ccggcgcggt gtgcctgccc ccggctggta
      361 cagccaaagg catccatctt cgtgacgtta cgaccgtaac ggagaatggc ctgcgtgtcc
      421 tccacaagct ctgtcgccgt ctcccagccg ttatccgggt caatccagtt cacctcaacg
      481 gcattatggc ggtccttcag ggcgctgaag ctgtagcgga acggcgcgcc atcatccggc
      541 atcaccacat tactgcggtt ataggtccac accttatctg atggtcggtc ctgcacgaac
      601 gtcagcgtct gcccgttcca taccggcata cagcgcatcg ccgagcagaa atcactgagc
      661 acatcccacg ccttacgctg cgtggtcagc caggcattac aggtgatgcg cggctctgtg
      721 ccgccaaagc cgtccggcac tgactggtcg cagtactggc cgatgacata cagcgcccat
      781 ttatccacat ccgccgcacc aagacgtttc cccatgccgt agcgcggatg ggtcagcata
      841 tcccacagac accaggccat gttgttgctg tatgccggtt taaacgttcc gtcccagata
      901 ccgctgtatt gccgcgtctg cgggttataa ttcgacggca cctgcagaac acgcccgcgc
      961 agatgataat tacggctcac cttctggctg ccgaactgct ccgagtccac ctgcacgccg
     1021 accagtgccg tgttcgggta gcactgtttc acatcgataa tttcggtgta tgacgaccag
     1081 agcgttttgt tctgcagctg gtctgtggtg ctgtccggcg tcatcctgcg catccggata
     1141 ctgaacgggc gcggcggcag gttatccacc accaccgagg ccagatactg tgaagtggtt
     1201 ttacccttaa tggtgatgtc tttttccgtc acccagccac cgttacgctg tatctgaacc
     1261 agcaggcgga cttccgatgg attcctgtcc ccctttgagg tggtttccac cagtgcctgt
     1321 acaccgaagg taaagcgcag acggtcgatg tttgcagacg taatggtgcg ggtgatcggc
     1381 gtgtcatatt tcacttctgt accgagcacc gtctcggaac cggaggattc aaatccctcc
     1441 ggcggtgtct gctcctgctc accggcccgg aacaccaccg tgacgccgga tatattggta
     1501 ttcccctcac tgtccagcac cggcgtactg ttcagcagca cgctttttaa tccatccacc
     1561 ggaccttcaa ccggcccttc gctgatggca tcgatcacac tcagcagctg cgtggacttc
     1621 aggttgtcct tcgcttcgcg cggggtatgc cccttactgc tgcctttacc cattcgtcat
     1681 gctccataaa cgataaaacc gcccggaggc ggtttcacat aaaacatttt gcatcagcga
     1741 ccaatcacca caacctgacc accgtcccct tcgtctgccg tgctgatctc ctgagaaacc
     1801 acgcgtgacc ccacgcgcat ttcaccgtac agaaccggca gaacattgcc ctgggcaacc
     1861 atgttatcca gtgaggagaa acaggtgttc tgtttgccgt tatccgttgt ctgtgtacgg
     1921 ggagttctgg ctttcggtgc cagcatctgc gccacaccac cgagcaccat actggcaccg
     1981 agagaaaaca ggatgccggt cataccaccg gccccaatgg ctgcccccca tgctgcaagg
     2041 gtggctccgg cggtaaagaa tgatccggca atggcggcag cccccaggac aatctggaat
     2101 acgccacctg acttggcccc ggcgactctg ggaacaatat gaatcacagc gccatcaggc
     2161 agagtctcat gtaactgcgc cgttaacccg gacgtgctga cgtcccgccc ggcaatccgt
     2221 acctgatacc agccgtcgct cagtttctga cgaaacgccg ggagctgtgt ggccagtgcc
     2281 cggatggctt cagcccccgt tttcacacga aggtcgatgc ggcgaccaaa tcgttgtaaa
     2341 tccccgtaaa ggcagatgcg cgccatgccc ggtgacgcca gagggagtgt gtgcgtcgct
     2401 gccatttgtc ggtgtacctc tctcgtttgc tcagttgttc aggaatatgg tgcagcagct
     2461 cgccgtcgcc gcagtaaatt gcggcgtgat tcggcactga tgaaccaaaa cagcacagca
     2521 gcacatcgcc cggctgtgcc gctgacaacg gcacctgata cagccccgtc gcctccagat
     2581 tatccagata gagattctgg ccgttacgcc accagtcatc ctcacgataa aagtccggca
     2641 tctcaatccc cgccagatga taagcatccc ggaacagtgt gtaacagtcc gtcacaccgt
     2701 gctcaaagcg ccgcccggtg agatgcggca cacagcggaa cttatgaatc gtcccccggc
     2761 agaccagcca ccacggcaaa tcactctgca cctgcagccg ccggtcggcc tcactcagcc
     2821 agggcagacc accggggtgg ctgtggacca gcgccacaat ctcaccctgc atttctgcct
     2881 gcagccagtc ttccggcgac atacggaaat acgcctccgg ctcaccggag atattcacgc
     2941 agggaaaata tctttccccc tccggcgtgc ttaccacgaa gccgcacgac tccgctggcg
     3001 cacatcgccg ggcgtgcgcc agaatcgctg attctgtctg tgtcatggga tttactgcga
     3061 aagtttgtta atggaaagga agccgccaaa gttgccgacg ttattgcgga acttacaacc
     3121 gctcaggcat ttgctgcatt tatccttcgt gatatcggac gttggctggt catattcatc
     3181 cgcgacagcc ggaccgctat aaccgcactc gtcaccgcga taggtccagg tgcaggtgtt
     3241 ggccagcatg atacgtcccg gaaaaacagc gccatccgtt tccgtcggcg tggacagtac
     3301 aaaggaggca ctcaccgcgc tcagttcgct gcactgctca atgcgccagc ggctgatcac
     3361 ctcctgctcc ggatcggcgt aactgtttcc gttgacgaag ttcaccgcat ccagaaaatg
     3421 ggcgtaaacc ttacgccgga ccaccgttcc gccgaccaga ctctgcatat cttccgccat
     3481 cccggtgacc ataccgtaca ggttagaaac cgtcagcgtg gggcgcgtac tggtgccttt
     3541 gccattcagt tcaaaaccgc
//
LOCUS       DAJTNN010000258         3418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTNN010000258 DAJTNN010000000
VERSION     DAJTNN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.263"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO150_003990"
     CDS             complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO150_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5517943.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(687..1160)
                     /locus_tag="OO150_003991"
     CDS             complement(687..1160)
                     /locus_tag="OO150_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5517944.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1492..2028
                     /locus_tag="OO150_003992"
     CDS             1492..2028
                     /locus_tag="OO150_003992"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5517945.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2143..2469
                     /locus_tag="OO150_003993"
     CDS             2143..2469
                     /locus_tag="OO150_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5517946.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(2657..2896)
                     /locus_tag="OO150_003994"
     CDS             complement(2657..2896)
                     /locus_tag="OO150_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5517947.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          943 a          761 c          722 g          992 t
ORIGIN      
        1 cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg tcggcttgaa
       61 tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt
      121 tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga gtaaagccac
      181 attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa
      241 ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt
      301 tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg
      361 agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt
      421 cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag
      481 atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt
      541 tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc atatgacgcc
      601 taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggtacaaaa gccgcgccag
      661 gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc agatttggta actataattt
      721 atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat tgctggggat ttcaggaaag
      781 taaacatcac cttccggctc gatgtctatt gtagatatat gtagtgtatc tacttgatcg
      841 atcaggcttt tgtatatctc cccaccacct gaaacaatga catgatccgt tattttcttt
      901 aggttggtta aagcatcttt aattgatgga aagatcaata cgttctcatt gtcagatgta
      961 aaacttgaac gtgttacgac cgcatacttt cggttgggta atgctcccat tgattcaaaa
     1021 gtcttgcgtc caaccaacag ccattggtta taggtaatag ctttaaacag gagctgttca
     1081 cctttggcac tccatggaat atcagggcca ttcccgataa ctccattctt cgatatagct
     1141 accattagtg atagtttcac aattcttcct cagaggttaa cattttatta gtaagcatgc
     1201 tcgttttgtc acccgctgat gcttaccgtt aattaatata atcacttgta ttaaatagac
     1261 tttctttaaa aatacaagac actctgttat tacaaatcgt gcatgccgtc tatcctgctt
     1321 gcacgatgca cattatctca cccaaagaac tcataacatt ttaatttatt tagcattaaa
     1381 ttaaaactct accggtaaac aagcatctct aggcgtaaaa aacaggcact tttttaaatc
     1441 ccagctagaa taggctgtat aggcagacag ttgcaagaca aggagcgttt tatgtctaac
     1501 agtccatttt taaattctat acgcacggat atgcgacaaa aaggttatgc gctgaaaact
     1561 gaaaaaactt acctgcactg gattaagcgt tttattctgt ttcacaaaaa acgtcatcct
     1621 cagaccatgg gcagtgaaga ggtcaggctg tttttatcca gcttagcaaa cagcagacat
     1681 gtagccataa acacgcagaa aatcgcttta aatgccctag cttttttgta caacaggttt
     1741 ttacaacagc cgttgggcga tattgattat atccctgcaa gcaagcctag acggctaccc
     1801 tctgttatct ctgcaaatga agtgcaacgc attttgcagg ttatggatac tcgcaaccaa
     1861 gttattttta cgctgctgta tggtgcaggt ttgcgcatta atgaatgctt gcgtttgcgg
     1921 gttaaagatt ttgattttga taatggctgc atcactgtgc atgacggtaa gggtgggaaa
     1981 agcagaaaca gcctactgcc cacgcgccta atcccagcaa taaaataact cattgagcaa
     2041 gcgcggctta ttcagcaaga cgacaactta caaggcgtag ggccatcgct gccttttgct
     2101 ttagatcaca aatacccttc tgcttatcga caagcggcgt ggatgtttgt ctttccctcc
     2161 agcacgctct gcaaccaccc gtataacggc aaattatgcc gccatcatct gcatgactcc
     2221 gttgcgcgaa aggcattgaa ggcagccgta caaaaagcag gcatcgttag caagcgtgtc
     2281 acttgtcata catttcgtca ctcgtttgct acgcatctat tacaagcggg gcgtgatatt
     2341 cgcactgtgc aagaactctt agggcataac gatgttaaga ccacgcaaat ctatacgcat
     2401 gtgttgggtc agcattttgc cggcaccacc agtcctgcgg atggactgat gctacttatc
     2461 aatcagtaaa agtagataaa tatctttatg tagccctgag tagcccctga tactttttat
     2521 ggtgaaaaag ataaacaatc atagagggga taaatatgag ccagttctac cattgttcag
     2581 ataagtttaa gcaagatact gtcaatcaca gtgaccattc atggtcatta cacttcctcc
     2641 agttgccaga caatgcctaa cataaaaacg caaaagccca aaagtcagag cttaggtgtt
     2701 ctaggcgttt tctaagcgct gaagaaatac gtcttaccgt agattgcgtg cgtttctgaa
     2761 gcacattttc cttatacatg gctttatccc aggcagcttc atcaagctct tggagtaaca
     2821 agccagtaat aatgcgacac tctcgggttt gtagcgagcc gctggtgaga tcggagttat
     2881 agcggaaagg ggtcatttgt aattatttgt gggtggagga tagttaactt attattgtag
     2941 ttggaagagg ctgctttcca aacattagca cctcgtaatg cttgttactg tatattaatt
     3001 tatgctctta gcgcatgtta atgggctatt acaatgcata atgtccacat caagataact
     3061 gtggcttaac acctgtacaa gcgaaaaaaa tcaatcacct gtccggaaat acgggatcac
     3121 tgtaaagtgt tggtgaggca gtctaggagg gggatttaat ttgggatact gatgaaagga
     3181 ttatgatcag ttggatatag gatcgtacta agctgagcgg caggttagct agattagtta
     3241 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     3301 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     3361 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagggct gccgaccg
//
LOCUS       DAJTNN010000259         3371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTNN010000259 DAJTNN010000000
VERSION     DAJTNN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.264"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            333..1253
                     /gene="lpxL"
                     /locus_tag="OO150_003995"
     CDS             333..1253
                     /gene="lpxL"
                     /locus_tag="OO150_003995"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5517948.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            1425..2649
                     /gene="mdtG"
                     /locus_tag="OO150_003996"
                     /pseudo
     CDS             1425..2649
                     /gene="mdtG"
                     /locus_tag="OO150_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            2732..3106
                     /locus_tag="OO150_003997"
     CDS             2732..3106
                     /locus_tag="OO150_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5517949.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(3107..3334)
                     /locus_tag="OO150_003998"
     CDS             complement(3107..3334)
                     /locus_tag="OO150_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5517950.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
BASE COUNT          818 a          847 c          868 g          838 t
ORIGIN      
        1 ggcgatgtgg aaatacttgt aaaacgaaat ggtggttcgc ggttcgctct cagccaacat
       61 tttggctttt agcgcgtcgt tggaaatgcg gttgtgtaac actggcatgg tgtacggttc
      121 ctgcgagatg ggaaagtaaa aatccgcggc atgatatagc aattatcgat aattaacatc
      181 cacacatttt acgctacatt tgcgcattaa aaattatttg ttatttacaa gcgcggcaat
      241 ttcgcccagt cttcagccac aaattttggt tgcgggcgaa aaaatgcgac aatacataca
      301 attgcccgaa taggttgaaa aacaggattg atatgacgaa tctacccaag ttctccaccg
      361 cactgcttca tccgcgttat tggttaacct ggttgggtat tggcgtactt tggttagtcg
      421 tgcaattgcc ctacccggtt atctaccgcc tcggttgtgg attaggaaaa ctggcgttac
      481 gttttatgaa acgacgcgca aaaattgtgc atcgcaacct ggaactgtgc ttcccggaaa
      541 tgagcgaaca agaacgccgt aaaatggtgg tgaagaattt cgaatccgtt ggcatgggcc
      601 tgatggaaac cggcatggcg tggttctggc cggaccgccg aatcgcgcgc tggacggaag
      661 tgatcggcat ggaacacatt cgtgacgtgc aggcgcaaaa acgcggcatc ctgttagttg
      721 gcatccattt tctgacactg gagctgggtg cgcggcagtt tggtatgcag gaaccgggga
      781 ttggcgttta ccgcccgaac gataatccac tgattgactg gctacaaacc tggggccgtt
      841 tgcgctcaaa taaatcgatg ctcgaccgca aagatttaaa aggcatgatt aaagccctga
      901 aaaaaggcga agtggtctgg tacgcaccgg atcatgatta cggcccgcgc tcaagcgttt
      961 tcgtcccgtt atttgccgtt gagcaggctg cgaccacgac cggaacctgg atgctggcac
     1021 ggatgtccgg cgcatgtctg gtgcccttcg ttccacgccg taagccagat ggcaaagggt
     1081 atcaattgat tatgctgccg ccagagtgtt ctccgccact ggatgatgcc gaaactaccg
     1141 ccgcgtggat gaacaaagtg gtcgaaaaat gcatcatgat ggcaccagag cagtatatgt
     1201 ggttacaccg tcgctttaaa acacgcccgg aaggcgttcc ttcacgctat taaatctccc
     1261 ctgccggatg cttcagaatg gcatccggca ttaccacagc aaatccccct gatttagcga
     1321 taaaagctct ctggattgcg ccccctggaa gtcgggcgca taattagtgt gcttatcttt
     1381 tcttcttatg ttcaccgcgc ctggcgcacc aacagcggat tgctatgtca ccctgtgaaa
     1441 atgacacccc tataaactgg aaacgaaacc tgatcgtcgc ctggctaggc tgttttctta
     1501 ccggtgccgc cttcagtctg gtaatgccct tcttacccct ctacgttgag cagcttggcg
     1561 ttaccggtca ctccgccctg aatatggtcc ggtattgtct tcagcattac atttttattt
     1621 tcggccatcg cctcaccgtt ttggggtgga ctcgccgacc gtaaaggccg aaaactcatg
     1681 ctattacgct ctgccctcgg catgggcatc gtgatggtgt tgatggggct ggcacaaaat
     1741 atctggcagt ttttgatcct gcgggcgctt cttgggttac ttggcggatt tgtccccaac
     1801 gctaatgctc ttatcgccac acaagtaccg cgtaataaaa gcggctgggc gctgggtacg
     1861 ctctccacag gcggcgttgg tggtgcgttg ctcggcccaa tggctggcgg cctgctcgcc
     1921 gatagctacg gcttacgtcc ggtattcttt attaccacca gtgtgctcat actctgcttt
     1981 ttcgtcaccc tgttttgcat cagagaaaaa ttccagccgg tcagcaaaaa agagatgctg
     2041 cacatgcggg aagtggtgac atcacttaaa aacccgaaac tggtactcag cctgtttgtc
     2101 actacgttaa tcatccaggt ggcgacgggc tcaattgccc ccattctgac gctgtatgtc
     2161 cgcgaactgg cgggtaacgt cagtaacgtc gcctttatca gtggcatgat cgcctcggtg
     2221 ccaggcgtgg cggctctgct aagtgcacca cgactcggca aacttggcga tcgaatcgga
     2281 cccgaaaaga tcctgattac agcgctgatc ttttctgtac tgctgttgat cccaatgtct
     2341 tacgttcaga cgccattgca acttgggatt ttacgttttt tgctcggtgc cgccgatggt
     2401 gcactactcc ccgccgtaca gacattgttg gtttacaact cgagcaacca gatcgccggg
     2461 cgtatcttca gctataacca atcgtttcgt gatattggca acgttaccgg accattgatg
     2521 ggagcagcga tttcagcgaa ctacggtttc agagcggtat ttctcgtcac cgctggcgta
     2581 gtgttattca acgcagtcta ttcatggaac agtctacgtc gtcgtcgaat accccaggta
     2641 tcgaactgat ttttcgcctt tcatacttgc aaaagcggag aatcagctat ccttttccct
     2701 gaaacctcat caactcaaag ggagaatcgt gatgaccatg tacgcaacgc ttgaagaagc
     2761 cattgacgct gcacgcgaag aatttcttgc agacaacccc ggcatcgacg ccgaagatgc
     2821 gaatgtgcaa cagttcaatg cccaaaaata cgttttgcag gacggcgaca tcatgtggca
     2881 agttgagttt tttgccgacg aaggggaaga aggtgaatgt ttacctatgc ttagcggtga
     2941 agccgcgcaa agtgtttttg atggcgacta tgatgagata gagatacgcc aggagtggca
     3001 ggaagagaat acattacatg aatgggacga gggggaattt cagcttgagc caccgctgga
     3061 taccgaggaa ggacgcgcag cagctgatga gtgggatgaa cgttaatcac tcatacgggc
     3121 catgatgaat gtcgatcggc agcagtaaag tatcaaaaac cagggagaat ggcagatcaa
     3181 ggattgtgac ataacgccag gcggagtcac gcacatccca ttgcacaccg ggataatatt
     3241 ggttgccatg cccctgcccc ggtatagtgc gactaataat gctgccacag ccactcagca
     3301 aggtcaccat gatgctcacc acaattaaac gcatttgata cctcttccgt aaaaaaatac
     3361 cggcataacg c
//
LOCUS       DAJTNN010000260         3362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTNN010000260 DAJTNN010000000
VERSION     DAJTNN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.265"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..274)
                     /locus_tag="OO150_003999"
     CDS             complement(<1..274)
                     /locus_tag="OO150_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517951.1"
                     /translation="MLEIVLSPVKAQQFTVTLDAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRL"
     gene            complement(274..984)
                     /locus_tag="OO150_004000"
     CDS             complement(274..984)
                     /locus_tag="OO150_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517952.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FIVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(981..3002)
                     /locus_tag="OO150_004001"
     CDS             complement(981..3002)
                     /locus_tag="OO150_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5517953.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRLANAKAAFR
                     AGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMRKLAVIFPRLTKSEQTAYGNALGF
                     SYAGQQFLGSGHALKDVDEFTSRSEVTPGRTALARKLRKELVELDQTWSGIGMTISTA
                     LMPYALKLSHWLETLGDWMQQHPEEVNRFITTFLNDVESVASLANKAAGELGGWQNVI
                     ITLIGLKVASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDWLDSHGFF
                     WASDGTFFFNKKEMETYQTKLDAGEKPGNITRAQSPTVWQQGMLDTQASQATGREAAF
                     GESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPPGLLRSVA
                     ITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKLGGLIRFF
                     HGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIRSLHPAPV
                     SQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(2986..3168)
                     /locus_tag="OO150_004002"
     CDS             complement(2986..3168)
                     /locus_tag="OO150_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5517954.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT          786 a          982 c          806 g          788 t
ORIGIN      
        1 agagccggta ccgggatccg agcccccgcc agtcagggtc actattcccc tccgtgtcgg
       61 taaaaaacag gtccccctga aacggcaggt atccgtaccg gacaatccgg ttattattca
      121 gacacagcac gccatacagg cacggttcac cattaacaat gatatcgata tacatccccg
      181 tagtacgctg attcaggcga atggtgcaga cctgagcgtc cagtgtcacc gtaaactgct
      241 gggctttaac gggagataaa acaatttcca gcatcaggtg atccccctgt tcgtggcact
      301 tcgtctgtca gcgtctgacg gttgtgtcac cgacgccgta actggctgcg ttttaaccga
      361 tgcggcccct tttgctttat cgttgtccgt gggagacgac ttatccgtac ttcccactga
      421 cacctctcct gtattcatta cggtctgaaa taccgcagta accgtcagta aggtcggacc
      481 attattactt cgcattcggt agtcatattt cacaaggtcg caggatgtcc atgtcttgtc
      541 cggcgtctca atatcgtaga gtcctgccgt ggtacgcatc cgttcaagcg tttccagcac
      601 attcgtccgc gaggtggtgg aaaaatttgt caggttcggg acggatccgg aaaaggccgt
      661 ccacccctct acaatgaaag tcacatgcag ttccggcgat ctctggattt tattaaaggt
      721 ggtataagcc ccctgctcca cggggacggt ggaaacagaa gcctccgcgc ccacctcaac
      781 gacaacaaaa gaatccgggg agaaaggttt gccacccttc tgatgggcac ctgccggatc
      841 attccgtgcg taataaatac cgaatgacgg tgccagtaca ctattaatga gtcccaggac
      901 accgccgcca cgaactgcac tcagtacgtt actttcactg agcgaaaagt tattcaggga
      961 aatattatcg aaagaaaaac tcatcctgtt accccgctgg aataaaccga cacaagcgcc
     1021 gaattcgtga tacgccgacg tgcgtcatcg gtaataccct tcacattgtc cgacgttgtg
     1081 gtgacattca gtgtcccgat atgcgtggtt tccgttacgg tggactgcga tacaggcgca
     1141 ggatggagcg accgtatggc cattgccgca cccggataag gcaggttcgc cagcacccgc
     1201 ggaatatagt tgcgggtctc ctccggagca gcagccagtc ccttacgctg aacatttccc
     1261 tctccccagt tatatgccgc cagagcctta gccagatcgc catgaaaaaa acgtatcagg
     1321 ccaccaagtt ttctcgcggc ggcatcagcg gctttttcgg gatcaaaggc atcgttcccc
     1381 ctcagaccaa attcactggc cgtcttcggc atgaactgaa acagtcccat cgcaccagca
     1441 ggtgagacgg caaactgatt accacgggat tcggtgatcg caacgctgcg cagcagtccg
     1501 ggcggcaggt tatatttttc ctccagttga gacagtttcg gttgcagcca gcctaacagg
     1561 gcttccccgg tcttcgtcgg tcgctggcgg ttttgcatgg cattaccgag tttttcctgc
     1621 gtcgcacgca taccctgtag ccaggattca ccgaaggctg cttccctccc ggttgcctga
     1681 gaagcctgag tatccagcat gccctgctgc catactgtgg gtgattgtgc ccgggtgatg
     1741 ttgccaggct tttctccggc atccagtttt gtctgatacg tctccatctc tttcttattg
     1801 aaaaagaaag tcccgtctga agcccagaaa aaaccatgtg aatccagcca gtccttattt
     1861 tccctgccaa caatggatgt cagtaatccg tcaacaaccg ggtaaagcgc cgttatcgca
     1921 aaaagaaggc ctccggggcc actgagggct tttgtcagcc cccgcaccca tgacgccact
     1981 ttcagcccga tcagcgtaat aatgacattc tgccagcccc ccagttctcc ggcagcctta
     2041 ttcgccaggg aggccactga ctcaacgtca ttcagaaatg tggtgataaa cctgttcact
     2101 tcctccggat gttgctgcat ccagtcaccg agtgtctcca gccagtggct gagcttcagc
     2161 gcgtacggca tcaacgcggt gctaatcgtc atcccgatgc cggaccaggt ctgatcgagc
     2221 tcaacaagct cttttcgtag cttacgggcc agcgctgtac gccccggcgt aacctccgaa
     2281 cgggaagtaa attcgtccac atctttcagc gcatgaccgg agccaagaaa ctgctgtccg
     2341 gcataactga agcccagcgc gttgccgtag gcagtttgtt cggactttgt cagtcgcgga
     2401 aaaataaccg ccagcttgcg catgatggct tcagtgctgt cagtatttaa atcaacacta
     2461 atgcccgccc tggctgcaac ctgaaacaaa tcctgtaaca caggatcaaa ggaccttccg
     2521 gctctgaagg ccgcttttgc atttgcaagc cgggaaaatg ccccagacat ttccccttca
     2581 tcaacattat ttgcccgcgc agcccgtaac catccgtcca gatttctggc cttcatgcca
     2641 aaagcatcgg attccacgga cagacggttc agatcacggg caaaacctgt taccaggttc
     2701 ttcagccccc ccagcgtcag actgacaccc gcaagagcca gaatctcgct acggatccct
     2761 gaaaaaaaac gggacgcccg tttcccggca tcttccattc gacgggccgt tttatcagca
     2821 tcttttccgg ttttttccag tgattcactg gttttcttct gtccctgact gaatgtgtca
     2881 gcgacacctt ccatcaccgt cgtgagccgg ttaagggcag catgtactgc ctgttccccg
     2941 gcagtaaagt ccctgtcatc aatcccgagg gacagaacaa gctcatcaag tacctgcgcc
     3001 atttctgttc tcctgtatca cctgttcgtt atgggcatcc acatgaatta tttccagcag
     3061 atcccataag tcctgcacac caaggacaga atccagttca gcttttgaag ccttaccgga
     3121 ggagataacg gtcgcaatgg tacggggaac attaacgtaa tccaccaccc cgaacggtct
     3181 gtcggggtcg agataacgcg gggggatatc tagctgacga cggtagcgaa aaaatccaca
     3241 tgcagcttaa agacctccgc acgcagatta agacgggtaa tgatttcttc aatatcctct
     3301 tcaacaagcg gtctccggat gctgcgtttt ttcggatcgg gaacaaactg cacacagtcc
     3361 at
//
LOCUS       DAJTNN010000261         3342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTNN010000261 DAJTNN010000000
VERSION     DAJTNN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.266"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1333)
                     /gene="maeB"
                     /locus_tag="OO150_004003"
     CDS             complement(<1..1333)
                     /gene="maeB"
                     /locus_tag="OO150_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5517955.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKSFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKR"
     gene            1622..2572
                     /gene="tal"
                     /locus_tag="OO150_004004"
     CDS             1622..2572
                     /gene="tal"
                     /locus_tag="OO150_004004"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5517956.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            2592..>3342
                     /locus_tag="OO150_004005"
     CDS             2592..>3342
                     /locus_tag="OO150_004005"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5517957.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTGYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGS"
BASE COUNT          803 a          877 c          852 g          810 t
ORIGIN      
        1 cgcgcttcgg cgctttgcga gcctgggaga aaatcggctt cataaacagg ttggttttgt
       61 aaacgaactc agtcagcttg tcgatgtaga cgtcgaaatc agcaatcgga cgcgtcgcca
      121 cgcccgactc catcgcggct ttagcgaccg caggagcgat cttaacgatc aagcgcggat
      181 caaacgattt tggaatgatg tattccggac caaagctcag atcctgatcg ccatacgctg
      241 aagccaccac ttcgctctgt tccgcatggg cgagttctgc aatcgcacgt accgccgcca
      301 gtttcatctc ttcgttgatg gcggttgcgc caacgtccag cgcgccacgg aagatgaacg
      361 ggaagcacag gacgttgttc acctggttcg gatagtcaga acgaccggtg caaatgatgg
      421 catccggacg cacttctttc gccagcggcg gcagaatttc cggttccggg ttcgccagcg
      481 ccaggatcat tggcgcacga gccattttct tcaccatttc ctgggtcagc actttcgggc
      541 cggaacatcc gaggaaaata tccgcgcctt caatcacatc atcgagggta cgtttgccgt
      601 cgtccaccac cgcatatgcg gctttggttt ccgccatgtt tggctcacgg ccctgataga
      661 taacgccttt tgaatcgcaa accacgatgt tatgtttttg cagacccagc gctaccagca
      721 ggttcataca ggcgattgct gcggcacccg cgccggaaac caccatccgc acgtcggaga
      781 tgtttttctc caccacgcgc aagccgttga ggatggcggc agtgctgatg attgccgtgc
      841 cgtgctgatc gtcgtggaat accggaatat tcatccgctc gcgcagtttc tgttcaatat
      901 agaaacattc tggcgcttta atgtcttcga ggttgattcc gccgaaggtc ggttcgagcg
      961 cagcgacaac ttcaataaat ttgtccgggt cgagttcatc aacttcaatg tcaaatacat
     1021 caatcccggc gaatttctta aacagaacgc ccttgccttc catcaccggt ttgccagcca
     1081 gcgcgccaat gttgcctaac cccagcaccg ccgtaccgtt agagatcacc gccaccaggt
     1141 taccgcgcgc ggtatatttg taggctttta acgggtcttt ttcgatttca agacaaggtg
     1201 cggcaacgcc tggtgagtag gccagcgcca gatcgcgctg cgttgccaga ggcttggttg
     1261 gagaaacctg gattttccct ggaactggaa attcatggaa atcaagtgca ctttgtttta
     1321 actggtcatc catttggttg ttcctttcac gtaacgttca caaataaagt gtgtgggcaa
     1381 cagcccctgc ccacaacgtg gcgcacatta ttaccccgcc ggagtctaca gactttgagc
     1441 aagtccaaac tctcaccatt aatataatgt tttggtaata atcctataac actgatgtta
     1501 cctgcttaat ccagcaatac catgcctgtc tgctatgctt ttttgatgcg tttagcgaaa
     1561 tttctcagaa gtgtgaatta acgcactcat ctaacacttt acttttcaag gagtatttcc
     1621 tatgaacgag ttagacggca tcaaacagtt caccactgtc gtggcagaca gcggcgatat
     1681 tgagtccatt cgccattatc atccccagga tgccaccacc aatccttcgc tgttgctcaa
     1741 ggctgccgga ttatcacaat atgagcattt aatagacgat gctatcgcct ggggtaaaaa
     1801 aaatggcaag acccaggaac aacaggtggt cgcagcgtgt gacaaactgg cggtcaattt
     1861 cggtgctgaa attctcaaaa tcgtacccgg tcgcgtgtca acagaagttg atgcacgcct
     1921 ctcttttgat aaagaaaaga gtattgagaa ggcgcgccat ctggtggact tgtatcagca
     1981 acaaggcgtt gagaaatcac gcattctgat caaactggct tcgacctggg aaggaattag
     2041 cgcggcagaa gagctggaaa aagaaggtat taactgcaac ctgacgctgc tgttttcttt
     2101 tgcccaggcg cgggcctgtg cggaagcagg cgtttttctg atttcgccgt ttgtcgggcg
     2161 tatttatgac tggtatcagg cacgcaagcc gatggacccg tatgtggtgg aagaagatcc
     2221 gggcgttaaa tcggtgcgca atatctacga ctactataag caacaccact atgaaaccat
     2281 tgtgatgggc gcgagcttcc gtcgcaccga acaaatcctc gccttaaccg gctgcgatcg
     2341 actgactatc gcaccgaatt tgctgaagga gctgcaggaa aaagtttcgc cagtggtacg
     2401 taaattaatc ccaccttctc agacgttccc acgcccagct cccatgagcg aagcggagtt
     2461 ccgttgggag cacaatcagg atgcgatggc ggtagaaaaa ctgtctgaag gcattcgtct
     2521 gttcgccgtt gatcaacgca aactggaaga tcttcttgcc gccaaactat aaaccagcca
     2581 cggagtgtta tatgtcccga aaagaccttg ccaatgcgat tcgcgcactc agtatggatg
     2641 cggtacaaaa agccaactct ggtcatcccg gcgcgccgat gggcatggct gatattgccg
     2701 aagtgctgtg gaacgatttt cttaaacata accctaccga cccaaccggg tatgatcgcg
     2761 accgctttat tctttccaac ggtcacgcgt cgatgctgct ctacagtttg ctgcatctga
     2821 ccggttacga cctgccgctg gaagagctga agaacttccg tcagttgcat tcgaaaaccc
     2881 caggccaccc ggagattggt tatacgcccg gtgttgaaac caccaccggt ccacttggac
     2941 aaggtttggc gaacgccgtc ggactggcga tagcagagcg tacactggcg gcgcagttta
     3001 atcagccgga tcatgaaatt gtcgatcact tcacctatgt gtttatgggc gacggctgcc
     3061 tgatggaagg tatttcccac gaagtctgtt cgctggcggg cacgctagga ctgggcaagc
     3121 tgattggttt ttacgatcac aacggtattt cgattgatgg agaaaccgaa ggctggttta
     3181 ccgacgatac ggcaaaacgt tttgaagcct atcactggca tgtgatccat gaaatcgacg
     3241 gtcacgatcc gcaggcggtg aaggaagcga tccttgaagc gcaaagcgtg aaagataagc
     3301 cgtcgctgat tatctgccgt acggtgattg gctttggttc gc
//
LOCUS       DAJTNN010000262         3341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTNN010000262 DAJTNN010000000
VERSION     DAJTNN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.267"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..145)
                     /gene="fliE"
                     /locus_tag="OO150_004006"
     CDS             complement(<1..145)
                     /gene="fliE"
                     /locus_tag="OO150_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="forms a junction between the M-ring and FlgB during
                     flagella biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5517958.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDT"
     gene            360..2018
                     /gene="fliF"
                     /locus_tag="OO150_004007"
     CDS             360..2018
                     /gene="fliF"
                     /locus_tag="OO150_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR5517959.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     LSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2011..3006
                     /gene="fliG"
                     /locus_tag="OO150_004008"
     CDS             2011..3006
                     /gene="fliG"
                     /locus_tag="OO150_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5517960.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     VATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            2999..>3341
                     /gene="fliH"
                     /locus_tag="OO150_004009"
     CDS             2999..>3341
                     /gene="fliH"
                     /locus_tag="OO150_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="binds to and inhibits the function of flagella
                     specific ATPase FliI; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5517961.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEADPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAKAKSQQAPIHARMQQLV
                     SEFQTTLDALDS"
BASE COUNT          838 a          879 c          952 g          672 t
ORIGIN      
        1 gtgtatcgct tatgcgatcg agcgcggcgt gcagctgccc ggcaaaacta atggtcggtt
       61 gcggcagtga atcctgcgca cgcgcactca tcgccgtagc ctgtaactgg ctgataaccc
      121 cttcaatccc ctgtatcgct gacattttcg tctcctggat aatttctggt agcaaagcct
      181 accagtaagt caataagaca aaggcgctaa atagcaacaa aaaaacgggt ttattggcgg
      241 atagaaaaaa acgaaagcac aaataatggc agcgtcaatt tttcgagttt gctgacccgg
      301 gagtgagtct tgttccactt tgccaataac gccgtccata atcagccacg aggtgcgcga
      361 tgaatgcgac tgcagcccag acaaaatctc ttgagtggct taatcgtctg cgtgcgaatc
      421 cgaaaattcc attgattgtt gccggttccg cggcagtggc ggtcatggtc gcactgatcc
      481 tgtgggcgaa agcccccgac taccgcacat tattcagcaa tctttccgat caggatggtg
      541 gcgcaattgt cagccaactg acgcaaatga atattcctta ccgcttcagc gaagccagcg
      601 gcgctattga agttccggca gataaagttc acgagctgcg tctgcgcctg gcgcaacaag
      661 gtttgcccaa aggcggcgcg gtcggtttcg aactactaga tcaggaaaag tttggtatca
      721 gccagttcag cgaacaggtg aattatcagc gggcgctgga aggcgagctt tctcgtacca
      781 tcgaaactat cggcccggta aaaggggcgc gcgtacatct ggcaatgccg aaaccgtctt
      841 tattcgtccg tgaacaaaaa tccccttctg catcggtgac ggtaaatcta ttacccggcc
      901 gcgcactcga tgaagggcaa attagcgcca ttgtacatct ggtttccagc gcggttgctg
      961 gtctgccgcc ggggaacgtc acgctggtgg atcagggtgg acatctgtta accctgtcca
     1021 ataccagcgg gcgcgatctt aatgacgctc agttgaaata tgccagcgat gtcgaaggcc
     1081 ggattcagcg gcgtattgaa gcgatcctgt cgccaattgt tggtaacggt aatattcacg
     1141 cccaggtcac ggcgcaactg gacttcgcca gtaaagaaca aacggaagaa cagtatcgcc
     1201 ctaacggtga tgaatctcat gcggcgcttc gttcacgcca gcttaatgag agcgagcaaa
     1261 gcggttccgg ttatccgggc ggcgtaccgg gggcgttgtc gaatcaaccg gcacctgcga
     1321 ataacgcgcc aatcagcacg cctccggcaa atcaaaataa ccgccagcag caggcgagca
     1381 ccaccagcaa tagcgggccg cgtagcacac agcggaatga aaccagtaac tacgaagtcg
     1441 atcgcaccat tcgtcatacc aaaatgaacg tgggcgatgt gcaacgtctg tcagtcgcgg
     1501 tagtggtaaa ttacaaaacc ttgccagacg gtaaaccatt gcctctcagc aacgaacaga
     1561 tgaagcaaat tgaagatctg acccgcgagg cgatgggctt ttctgaaaaa cgcggtgact
     1621 cgctcaatgt cgttaactcg ccgttcaata gcagtgacga aagcggcgga gaactgccgt
     1681 tctggcaaca gcaagcgttt atcgatcagt tgctggctgc cggtcgctgg ttgctggtac
     1741 tgctggtggc gtggctgctg tggcggaaag cggtacgccc gcagttaaca agtcgcgccg
     1801 aggcgatgaa agctgtacag caacaggcgc aggcccgtga ggaagtggaa gatgcggtgg
     1861 aagtccgcct gagcaaagac gaacaactcc aacaacgacg cgctaaccaa cgtctggggg
     1921 cagaagtcat gagccagcgt atccgtgaaa tgtctgataa cgatccgcgc gtggtggcgc
     1981 tggtcattcg ccagtggata aataacgatc atgagtaacc tgacaggcac cgataaaagc
     2041 gtcatcctgc tgatgaccat tggcgaagac cgggcggcag aggtgttcaa gcacctctcc
     2101 cagcgcgaag tgcaaaccct gagcgctgca atggcgaacg tcacgcagat ctccaacaag
     2161 cagctaaccg atgtactggc ggagtttgag caagaagctg aacagtttgc cgcactgaat
     2221 atcaacgcca acgattatct gcgttcggta ttggtcaaag ctctgggtga ggaacgtgcc
     2281 gccagcctgc tggaagatat tctcgaaact cgcgataccg ccagcggtat tgaaacgctc
     2341 aactttatgg agccgcagag tgccgccgat ctgattcgcg atgagcatcc gcaaattatc
     2401 gccaccattc tggtgcatct gaagcgcgcc caggccgccg atattctggc gttgttcgat
     2461 gaacgtctgc gccacgacgt gatgttgcgt gtcgccacct ttggcggcgt gcagccagcc
     2521 gcgctggcgg aactgaccga agtactgaat ggcttgctcg acggtcagaa tctcaagcgc
     2581 agcaaaatgg gcggcgtgag aacggcagcc gaaattatca acctgatgaa aactcagcag
     2641 gaagaagccg ttattaccgc cgtgcgcgaa ttcgacggcg agctggcgca aaaaattatc
     2701 gacgagatgt tcctgttcga aaatctggtg gatgtcgacg atcgcagcat tcagcgtctg
     2761 ttgcaggaag tggattccga atcgctgttg atcgcgctga aaggggccga gcagccactg
     2821 cgcgagaaat tcctgcgcaa tatgtcgcag cgtgccgccg atattctgcg cgacgatctc
     2881 gccaaccgtg gtccggtgcg tctgtcgcag gtggaaaacg aacagaaagc gattctgctg
     2941 attgtgcgcc gccttgccga aactggcgag atggtaattg gcagcggcga ggatacctat
     3001 gtctgataat ctgccgtgga aaacctggac gccggacgat ctcgcgccac cacaggcaga
     3061 gtttgtgccc atggtcgagc cggaagaaac catcattgaa gaggccgacc ccagccttga
     3121 gcagcaactg gcgcaactgc aaatgcaggc ccatgagcaa ggttatcagg cggggattgc
     3181 cgaaggtcgc cagcaaggtc atgagcaggg ctatcaggaa ggactggccc aggggctgga
     3241 gcaaggtctg gcaaaggcga agtctcaaca agcgccaatt catgcccgga tgcagcaact
     3301 ggtcagcgaa tttcaaacta cccttgatgc gcttgatagt g
//
LOCUS       DAJTNN010000263         3332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTNN010000263 DAJTNN010000000
VERSION     DAJTNN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.268"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..340
                     /gene="ldtE"
                     /locus_tag="OO150_004010"
     CDS             <1..340
                     /gene="ldtE"
                     /locus_tag="OO150_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5517962.1"
                     /translation="SVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTM
                     PYTLPAGFTQFKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEP
                     EQGNMLRARQ"
     gene            complement(404..640)
                     /gene="lpp"
                     /locus_tag="OO150_004011"
     CDS             complement(404..640)
                     /gene="lpp"
                     /locus_tag="OO150_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5517963.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(951..2363)
                     /gene="pykF"
                     /locus_tag="OO150_004012"
     CDS             complement(951..2363)
                     /gene="pykF"
                     /locus_tag="OO150_004012"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5517964.1"
                     /translation="MKKTKIVCTIGPKTESEEILAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2920..3129
                     /gene="fumD"
                     /locus_tag="OO150_004013"
     CDS             2920..3129
                     /gene="fumD"
                     /locus_tag="OO150_004013"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5517965.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELDKQAMETVINALVK"
BASE COUNT          909 a          700 c          802 g          921 t
ORIGIN      
        1 cagcgtgcgg acgggaacgc cggtgaaagt gatcaacgaa ccggtgaaat attccgtgga
       61 gcctaacggg atgcgttatg ttgaagtaca tcgaccacta tcggcagaag aacagcagaa
      121 cgttcagaca atgccataca cactgccagc aggctttacg caatttaaag acaataaggc
      181 tgtagatcag aagttagtcg ataaagcgtt gtatcgtcgg gcagggtatc cggtttcggt
      241 gagcagtgga gcaactcccg cagccagcaa tgcgccttca gtagagtcag cgcagaatgg
      301 tgaaccagag caagggaata tgttacgcgc gaggcagtag tggtaaatag cagacaaaaa
      361 aaatggcgca caatgtgcgc catttttcac ttcacaggta ctattacttg cggtatttag
      421 tagccatgtt gtccagacgc tggttagcac gagctgcgtc atctttagca gcctgaacgt
      481 cggaacgcat tgcgttcacg tcgttgctca gctggtcaac tttagcgttc agagtctgaa
      541 cgtcagaaga cagctgatcg attttagcgt tgctggagca acctgccagc agagtagaac
      601 ccaggattac cgcgcccagt accagtttag tagctttcat tattaatacc ctctagattg
      661 agttaatctc catgtagcgt tacaagtatt acacaaagtt ttttatgttg agaatatttt
      721 tttgatggga aggcacttat ttttgatcgt tcgctcaaag aagcatcgaa cgctgggttt
      781 tagataaaaa aattgagaga aaacaggatg cttccatcgg attcatctta gataaagtga
      841 gattatatag tgaaatccga tttgattttt tattgcttct ggttatcgat taaataaaaa
      901 aagcgcccat cagggcgctt cgatatacaa attaattcac aaaagcaata ttacaggacg
      961 tgaacagatg cggtgttagt agtgccgctc ggtaccagtg caccagaaac cataactaca
     1021 acgtcacctt tgtgtgccag accgctctgc agagccagtt ctttacccag acggtagaaa
     1081 tcatcagtag aagtgatctc tttaaccagc tgcggcacaa cgcctttgct cagtaccaac
     1141 tgatgagccg ttttttcgtt ggtggtcagt gccaggatgg tggcatccgg gaagtattta
     1201 cgtactgcgc gagcagattt accgccctga gtagcaacca cgatcagcgg agcatccagt
     1261 ttttcagcag tttcaacggc accacggcat accgcttcgg taatgcgcag tttacggttg
     1321 tcattgttga actcgagacg gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc
     1381 atgatagaaa ccgcttccag cgggtattta ccttttgcgg attcaccaga cagcatcact
     1441 gcatcagtac cgtcgaggat ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt
     1501 gggtttttga tcatggaatc cagcatctgg gtcgcagtga taacgacttt acgtgcacgg
     1561 atacattttt cgatcatcat cttctgggcg aagataactt cttctaccgg gatttctaca
     1621 cccaggtcgc cacgcgcaac catgatgccg tcagaggctt cgaggatttc gtcgaagttg
     1681 ttgaggcctt cctggttttc gattttggag atgatgtgga tgttttcgcc gccgtgcgct
     1741 ttcaggtgct cacggatttc gataacgtca gaacgcttac gaataaagga agcagcaaca
     1801 aagtctacgc cttgttcgca accaaagatc aggtcctgtt tgtctttttc agccagtgct
     1861 ggcagagcaa tggaaacgcc aggcaggttc acacctttgt tttcgcccag gtcaccgttg
     1921 ttcagcactt tacagataac tttgttacct tcaatggcgg taacttccat accgatcaga
     1981 ccatcgtcaa ccagtacggt gttgccaact gacaggtcag tagtgaaacc ttcatacgtt
     2041 accgcaacca tttcgctgtt gccgataaca gatttatcag tggtgaaagt aaaggtctga
     2101 ccagctttca gagaaacgtc gttaccgcct tccagtttca tggtgcggat ttccggacct
     2161 ttggtatcaa gcaggatagc ggcggtttta ccagttttgc tcatcacgtt gcgcagattc
     2221 tgaatgcgct gaccgtgttc tgcatagtca ccatgagaga agttcagacg cataacgttc
     2281 atgccagcgt ccagcatttt agctaatatc tcttcagatt cggttttcgg tccgatggtg
     2341 caaacaattt tggtcttttt catgacagtc ttagtcttta agttgagaag gatgggagaa
     2401 acttgctttc tgggcgcact gccggaaagc gaatccggta ttacgaaagt ggtggtgcct
     2461 cgctctaagg ataggtgaca tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc
     2521 ccaacgtgag tgcctgtttt tgagtcgttg agttctacag tccaaagtgc tgaaaccatt
     2581 caagagtcaa ttggcgcgca ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc
     2641 gtttcaaaaa atatacaact tgttagacaa taaattgtta tcaaagattt aacgttccag
     2701 ggaaaaggtt acgcaaaaac ttcgctgttt gcgcaacgtc tggtgccaaa gttgtcagtg
     2761 tgaggataag tgaaagttca tccggtgtat aaatatatca tcgctcaatt gctacagtta
     2821 aaataattta tcgcgttatt tgtctgattt tgtttgattt ttgtttaatt ggttagattg
     2881 atatatgctg atagttggca tgataaatag gtataaagaa tgggaaatcg gacaaaagag
     2941 gatgaactgt accgagaaat gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat
     3001 ctggggcagg aaccaaagca tattgttatc gcaggcgtat tacgtacagc attagccaat
     3061 aaacgcattc agcgcagtga gctggataag caggcgatgg aaaccgtcat taacgcactg
     3121 gtaaaatgac cattcaggat gaggcggtaa gcttcatcct gatattcttg cttcagttgc
     3181 ggtccagtta atcattctaa ataacaactg ctgaaattat acgaattgcg gtatgtattt
     3241 taatttataa cgataatatt atttttattt tctgcaatga acgtcacaaa taattgtcgc
     3301 ttaagttaaa tttatttcaa atgataataa at
//
LOCUS       DAJTNN010000264         3330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTNN010000264 DAJTNN010000000
VERSION     DAJTNN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.269"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..276
                     /locus_tag="OO150_004014"
     CDS             <1..276
                     /locus_tag="OO150_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5517966.1"
                     /translation="EYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQV
                     CGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(316..1530)
                     /gene="ydcO"
                     /locus_tag="OO150_004015"
     CDS             complement(316..1530)
                     /gene="ydcO"
                     /locus_tag="OO150_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR5517967.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVARATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAEILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTVASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITEMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNQY"
     gene            1583..2119
                     /gene="sutR"
                     /locus_tag="OO150_004016"
     CDS             1583..2119
                     /gene="sutR"
                     /locus_tag="OO150_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR5517968.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2192..>3330
                     /gene="rlhA"
                     /locus_tag="OO150_004017"
     CDS             2192..>3330
                     /gene="rlhA"
                     /locus_tag="OO150_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR5517969.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMNYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPA"
BASE COUNT          856 a          856 c          863 g          755 t
ORIGIN      
        1 gagtacaagc agctctggcg tggcggtcag gtgcttgctg ttccgccagc gtatacaagc
       61 cagcgttgcg cgtactgtgg tcatacagcg aaagagaacc gcctgtcaca aagtaaattc
      121 agatgccagg tatgtggata tacagcgaac gccgatgtaa atggcgctcg taacatttta
      181 gcggcggggc acgccgttct tgcctgtgga gagatggtgc agtcaggccg cccgttgaag
      241 caggaaccca ccgaaatgat tcaggcgaca gcctgaacgt agcagggatc cacgtctttc
      301 agggcgtgta ggatgtcaat attggtttct gtcagcgatt aaattcaaca ccacgtaaca
      361 aacgcctccg gcaattaatc cccagaacgc agaaccaatc ccgaccagcg tcaatccact
      421 tgccgttacc agaaatgcca ccaccgccgc gtcgcgctca cgctcattat gcagcgcctg
      481 atacaaactg ccgccaatgg tactcaacag cgccagacct gccagcatct ggatccaact
      541 tacgggcagg gcagccatca tctcggtaat ggcactacca aacagacctg cgagcaaata
      601 gaaaatgcct gcaacggcag ctgccagcca acgttgatct ttatccggat gcgcttccgg
      661 gctttggcaa atagccgcgg tgattgccgc aataccgacg gaataaacgc cgaaagggga
      721 aaaaaccagt gccagcaatc cagtaaatac aattaatggc gaaacaggag ccgaatatcc
      781 agctgctttc attgctgcga tacccggtgc gttttgcgat gccaccgtca ccagaaaaag
      841 ggggagtgca acgctcaggc tgtgagcaaa cgaaaaatca ggggtaatat aagtggggag
      901 aacgggttta aagacaacat cagttgtgac aacgtcacct tgcgcgatga cgatcacgat
      961 cccaataatc atcgcggcaa ttaccgcata gcgcggcgca acggccttgg ttgccagcca
     1021 taccagcaac atacttccac acaacgtaaa ttgaccgtcc agactggcaa acgcctgtaa
     1081 accaaagcgt aataaaatct cggcaagcat tgccgccgca agcgagtgcg gaataatgcg
     1141 catcagacga gcaaagagtc ccgttatgcc gcagaggact attaacgcgt tggtgacaat
     1201 aaaaacgccg atggcttcgt taagtgttag tccctgcaat ccggtgacca acaaagccgc
     1261 gccaggcgtt gaccatgcgg tgagaacagg tacgcgatac cataatgtca gagtcagcgt
     1321 actgacgccc attgccagcc ccagcgccgt catccagcca gagatttgtg cagtggtggc
     1381 tctggcgaca atcgctgctt gccagattat tgccgctgaa ctggcgtagc caattaatac
     1441 cgccagaaac cccgccagta gcgtgggtgg aggaatagaa aacagacgca tagtcacctc
     1501 gtacgttata gcgtacaggc accgtagcat ttgtccgtta taacgcacaa gtgataaact
     1561 tccgttttgc cggaggagtc gcatggaaaa tctcgctcgc tttttatcca ccacacttaa
     1621 acaactacgc cagcagcgcg gctggagtct ttcgcgactg gcagaagcga cgggcgtttc
     1681 taaagcaatg ttagggcaga ttgagcgtaa tgagtccagt ccgacggtcg cgacattatg
     1741 gaaaattgcc accgggttga acgtaccatt ttcaacattt atttctccgc cgcagtccgc
     1801 tacgccttca gtttatgatc cacaacagca ggcgatggtg atcacttcgc tgtttcctta
     1861 tgatccacaa ctctgctttg aacacttctc gattcagatg gcatctggtg caataagcga
     1921 atcgacgccc catgagaagg gggtaatcga acatgtcgtc gtcattgatg gacaacttga
     1981 tctgtgcgtt gatggggaat ggcagaccct taattgtggg gaaggcgttc gatttgctgc
     2041 agacgtcacg catatctatc gtaatggtgg ggagcaaacc gtacattttc attccctcat
     2101 ccattacccg cgcagttaag cgggaaaact atttcgcaac gtcgcgcttg tggctaaaat
     2161 agccgccatt tttcagctac tggataagaa tgtgaccgta tcttctcatc gacttgaact
     2221 gttaagcccg gcacgcgatg ccgccattgc ccgcgaagct attttgcacg gtgccgatgc
     2281 tgtttatatc ggcggccctg gttttggtgc ccgtcataat gccagtaata gcctgaaaga
     2341 tattgccgag ctggtgccgt ttgcccatcg ttatggtgca aaaattttcg tcacgcttaa
     2401 caccattttg catgatgatg agctggaacc cgcgcaacgg ctgattactg acctctacca
     2461 gaccggtgtc gatgcgctga ttgttcagga tatggggatt ctggaacttg atattccgcc
     2521 gattgaactg cacgccagta cgcagtgcga cattcgtaca gttgaaaaag cgaagttcct
     2581 ctctgatgtt ggcttcacgc agattgtgct ggcgcgagag ctgaatcttg atcagatccg
     2641 cgcgattcac caggctacgg acgcgaccat tgaattcttt attcatgggg cactgtgcgt
     2701 ggcctattcg ggtcagtgct acatttctca tgcgcaaaca gggcgtagcg ccaaccgtgg
     2761 cgattgctcg caggcgtgcc gtttgccata cacattgaaa gacgatcagg ggcgggtggt
     2821 ttcctatgaa aaacatctgc tgtcgatgaa agataacgat cagactgcca acctcggcgc
     2881 gctgattgat gctggtgtac gctccttcaa gattgaaggg cgttacaaag atatgaacta
     2941 cgtgaagaat atcaccgccc attatcgcca gatgcttgat gccattattg aagaacgtgg
     3001 cgatctggcg cgcgcttcat caggtcgtac tgaacatttc tttgttccat cgacggaaaa
     3061 gactttccac cgtggtagca cagattattt tgtgaatgcc cgtaaaggcg atattggcgc
     3121 gttcgattcg ccgaaattta tcggcctgcc ggtgggtgaa gtattgaaag tggcgaaaga
     3181 tcatctcgat gttgccgtca ccgagccact ggcaaatggt gatggcctga acgtgatgat
     3241 taaacgtgaa gtcatcggtt ttcgtgccaa tacggtcgag aaaaccggag aaaatcagta
     3301 ccgcgtctgg cccaatgaaa tgccagcaga
//
LOCUS       DAJTNN010000265         3242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTNN010000265 DAJTNN010000000
VERSION     DAJTNN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.271"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..197)
                     /locus_tag="OO150_004018"
     CDS             complement(<1..197)
                     /locus_tag="OO150_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5517970.1"
                     /translation="MGNYIRPLSDAVFTIASDDQWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGR"
     gene            complement(443..895)
                     /locus_tag="OO150_004019"
     CDS             complement(443..895)
                     /locus_tag="OO150_004019"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5517971.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(892..1944)
                     /gene="dinB"
                     /locus_tag="OO150_004020"
     CDS             complement(892..1944)
                     /gene="dinB"
                     /locus_tag="OO150_004020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5517972.1"
                     /translation="MKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTAN
                     YPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLD
                     EAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQ
                     FVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFG
                     RILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKV
                     KPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGLH
                     VTLLDPQMERQLVLGL"
     gene            complement(2003..2773)
                     /locus_tag="OO150_004021"
     CDS             complement(2003..2773)
                     /locus_tag="OO150_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517973.1"
                     /translation="MIYSNQLRYTGKDGVYFHLRDNVDAFLTLSATENMEFDSPMQVT
                     TQNMQSGQTVTDNVQRAPRTITISGVVVVGESGSILISRDTKLVENFIDTLENWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATG
                     KTATTNDGGATSKKNTGNTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSV
                     KNGESTFTASANEQASKVLKSGNALRKYSVNPNKKGTY"
     gene            complement(2931..>3242)
                     /locus_tag="OO150_004022"
     CDS             complement(2931..>3242)
                     /locus_tag="OO150_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5517974.1"
                     /translation="LKPAFTMDDVKGWFNSAAMNLANFQHPELNNIKQDSALTSADIQ
                     SLRDEISALSKRIQEPVKVSLGGEVAIKPDETSFMTFSSNIYDQYAEATLLSSSFPED
                     D"
BASE COUNT          858 a          751 c          716 g          917 t
ORIGIN      
        1 aaacgaccaa cagagaagaa cgctgcgcca atcggatagc cgcgtccggg atgtaaatct
       61 ggcatcccca ctacgcgctg catgttgggt aaatttgccg tggtgtgtaa ttgttggatc
      121 gctaaactct cgatccactg gtcatcagat gcgatggtaa ataccgcatc ggataaggga
      181 cgaatataat tgcccatata ccattccgta aatagactcg tcatacttca agttgcatgt
      241 gttgcgactt gaattattga gagtaacaat taaaaaatca ggaaatggca gacgaatcca
      301 gggcaagaaa aaaataatta atcctggaaa ggtctgcaaa gagtcattga taaaggattt
      361 gtcatgattt acctccttgc agtttgtgga ataagaaaac gcgcacatac taggcttgcg
      421 ttattttcat tgcaagctgg atttaatgtt gctgtttata acgcatataa aaattaataa
      481 accactctcc ccgacattca acgcgttgct gcttaactgt ctgaaaacca taacgttcaa
      541 aaaagggttt tgcggttatg cttgcgtcca ccgtaagttc ggattcagac ttaatcaaag
      601 gttttaacaa agcgctggca accccacggc gggtgtattc agggtcaaca aataacatat
      661 cgatatagtg ttcaacgcag gtaataaagc cgaccagttt tgcattaatg actgcaaccc
      721 gcacttgtga tttcgcgagt ttctccttcc agcgagattc gtcaatctgc gcccaggcgg
      781 caatttgttg tggtgaataa tgctgactgg cagtcatcgt aaccgctcta atgaaaatag
      841 cgcatagttg ctgaaaatcg ccaggctgat agtctcttat ttgtatgtta ttcataatcc
      901 cagcacgagt tgtctttcca tttgcgggtc aagcaacgtc acatgcagcc ccaccagtcg
      961 cacaccgcgc ccgccgcggc gttcatccca ggttttacgc gcggtggcga ttaagtcagc
     1021 tttattcagc cgcggccaga cgtgctcctg agtggtttgc tgaaaatcat caaactttaa
     1081 tttcaccccc tggcgagcaa tcagtaaatc aggtttcacc tttgccagac ggcgttcaag
     1141 ttccggatac agccgctcga taatcgcttc acattcagac cagtggtgga tatcttccgc
     1201 catcgtgcgt tccacgccga cggattttcg caaccgttcg ctgttaacgt cgcgctcgtc
     1261 aatcccctga ctacgctccc acaaaatgcg gccaaatttg ccaaagcgtt taagcagcat
     1321 caccagatca cacttttgta catcaccgca ggttcgtagc cccatcgctt ccagttttgc
     1381 cgccgagact ttgccgacgc cggggatttt tgccagtggt aaggtttgta aaaatgccgg
     1441 aacttctgcc ggcgtaatca caaactggcc gttgggttta ttcatgtcgg aggcgatttt
     1501 ggcgagaaac tttacgggtg ccacgcccgc agacgccgtc agttgcagct cgttgaaaat
     1561 cgtctggcgg atttcctggg cgatgagggt cgcagaaccg tggcaatgga cgctatcggt
     1621 gacgtcgaga taagcctcat ccagtgacaa cggttcaata cgcgaggtgt agcgcgagaa
     1681 gatttcgcgg atatgatttg aggcttcttt gtaggcgtca aagcgccccg gaagcaaggt
     1741 gagatgcggg cataatttga gcgccatccc tgtcggcata gcgctacgta cgccaaattt
     1801 acgcgcggga taattggcgg tactgatcac cccccgacgt tcgcggctgc cgccaatagc
     1861 aatagggata tcgcgcaggg cgggattgtc gcgcatctcc accgccgcga aaaagcagtc
     1921 catatccaca tgaataatct tcactttctt atacaccctg tatatttata catgcaaaga
     1981 gattatacac acaaaagcaa aatcaataag tgcccttctt attcggattc acagagtact
     2041 tccgtaaagc attacccgac ttaagcacct ttgaagcttg ctcattagct gaggctgtga
     2101 aggtgctctc accattttta acgctaacgc tcttagagca cgttacacgg gcttttagcg
     2161 cttcatctga taactcactt gccgaatagc taaaaagctc tttacaggtc agcatagggc
     2221 ttccaaggct cgttgtagtg ttgccagtat tctttttact ggtagcacca ccatcattcg
     2281 tggtagcagt cttgccagtt gcagccgaaa tatcagtctg acccacaata gctttgaagt
     2341 taatttcctg aaaagttagc tggattctta gaccatttga aataccaaca tctttagagg
     2401 ctttaaagct cgtgataatg gaatcatcaa ttttaattcc gtctttacag atgaccgaaa
     2461 taatctgctt ctggtcacgc cagttttcca gagtgtcgat gaagttctcc accagcttag
     2521 tatctcgtga gataaggata ctaccggatt caccaactac aacgacacca ctaatagtga
     2581 ttgttctggg tgctctttgc acattatctg tgacggtttg ccctgattgc atgttctgtg
     2641 tagtcacctg catagggctg tcaaattcca tgttttcagt tgctgataag gttaagaagg
     2701 catctacatt atctcttaag tggaagtaaa cgccatcttt gccagtgtaa cgaagttgat
     2761 tagagtaaat cattaaattc tcctttgatt tcaaaggctt agtgtatttt aatcattttt
     2821 aaccaaacct ttgatatata agtattttct ctgttaagcg tgagctacgt gaaaaataat
     2881 ttttggtgtg ggtattaact ttgctttatt cacgtagcat tcgactatta ttaatcatct
     2941 tctgggaatg atgaacttaa tagcgtagct tctgcatatt ggtcgtagat attactgcta
     3001 aaggtcataa agctggtttc atctggttta atggcaactt caccaccaag tgaaactttc
     3061 acaggctcct gaatacgttt gctcaaagcg gagatttcat ctctcaggct ttggatatct
     3121 gcactcgtta atgctgaatc ttgcttaatg ttgtttaatt ctggatgctg gaagttagca
     3181 agattcatag cagcactatt aaaccaccct ttaacatcat ccatagtgaa agcgggttta
     3241 ag
//
LOCUS       DAJTNN010000266         3233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTNN010000266 DAJTNN010000000
VERSION     DAJTNN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.272"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(2..218)
                     /locus_tag="OO150_004023"
                     /pseudo
     CDS             complement(2..218)
                     /locus_tag="OO150_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(474..1454)
                     /locus_tag="OO150_004024"
     CDS             complement(474..1454)
                     /locus_tag="OO150_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5517975.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(1454..2653)
                     /locus_tag="OO150_004025"
     CDS             complement(1454..2653)
                     /locus_tag="OO150_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5517976.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(2975..>3233)
                     /locus_tag="OO150_004026"
     CDS             complement(2975..>3233)
                     /locus_tag="OO150_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5517977.1"
                     /translation="CRELLALLTPFCIGMVTSDDWGSYAREVPKEKHLTGKIFTQRIE
                     RNNRTLRTRIKRLARKTICFSRSVEIHEKVIGSFIEKHMFY"
BASE COUNT          905 a          747 c          677 g          904 t
ORIGIN      
        1 attacatcat ggtcgagttt cctgatttcc cgggcccagt aatgagatgc cccgccagcc
       61 tccatcgcaa cagtcatttt aggctgttgc accagccagt taagcagttg attgcgagaa
      121 agtttggtct ttcctgcggg tttgccatga gcattgatac tgaaaagata aaagttcgtt
      181 ttagccagat caatgccggt gattttctgt tcagacatgg tatcccgctt acttttgtgg
      241 ttaaagttta tccatcatgg cgcaattgaa ggctgccgtg aaagaggggg aagactatct
      301 catcggttac tgttaaacgg ctaagagaaa ctatcttttt caagcaaaga agatacggtg
      361 aaacaggcga tttcaccgtg aaatactgtt caaaaaaaac gctaataaaa gcaaaatttc
      421 ttttaaaaca gccaattaaa aactggtgag ttgctggcga aaaacttgaa ttttcacagc
      481 gaattcagat tatcttttag tacaagagca atcatacgat ctaacttatc ctgaacttcc
      541 aacggtaatc tgccgaattc atatgagaac gtacgtccct ttactcgttt tcgagcaaat
      601 ttatctttac tatcaaattt ccataacaaa gttacaaccg ctttatcctt tactcctgaa
      661 tcaagcaatg aagacatttc cttggttata agccgcataa tactgttttt aacctcgtct
      721 tcagaaagat tgtcatcagc agtaataagc tcaatactcg gcgagatatt cttcacaact
      781 tcatcaatag atatatcacc cagcctcagt cgttcactta attcagccag ttgcctgtaa
      841 tcggcgtatg tcagctcaga ctgaacagga aaaagagaca caaaatcttt aggtacggac
      901 gctgcttgga gggcacgagt caccttagct gccgacaatc cctctctttc tgcaatttgc
      961 ttctggttca taccggcctc tttgagacgt actaaacgca acccaatttc acgaatatta
     1021 tgttcaagtg acgtctgcaa atctttcgcc agatgctgag cttcagaaac agtaagctca
     1081 tcatccgtta caagtacacg taatccaacc ttgcatagca aagctgctgc ccgacgacgg
     1141 gaaccatcca aaatctcaat aagatcgcca gtccgaatcc caatacatgg atagaattgt
     1201 tgtaaacgta tagttctggt gatgtctttt agtgagtccg gcgtcagggc tgtctgttcg
     1261 cggccattca cctcttcaac cacaaaagtt tgagtgtcca ctttgtcatg atcaaccttg
     1321 atctcagtaa attttgcctt tcggccagta ttgagggtaa atacatgaac tggagccgat
     1381 atttcttcag tattattcag gatatttgta ttcagcgtac ggccaatagt cggtcggtgc
     1441 tttctgtttt ccatcagtag ttggccctta taaattcaat acgatcaaaa actgctttag
     1501 cgaaatcttc tgctgccata cgtgcatttt tcaaagcttc accactgcca acataagtaa
     1561 caggattcgc agatatcacg gtatcaaacg actcgccgct tctctcaaaa ccatcaagac
     1621 gtggcattga aacatcaagc atgtcaccac cgaaaatctc ttttgtcagg ctatggcagt
     1681 atttatgatc tgacttattc gccagtttcg acatgaagcc aatatttgcc tgcaaacggc
     1741 atgaacatcc atcctgttca ataatctgta caagttcagg taaacgagcc aaatatttaa
     1801 gcgtggaatg gaaatcaact tgtgctggcg gcaccggtgt aaacatgata tctgcggcag
     1861 caatggcatt tttcaaaaat gcgtccagat gcgggcctgt atcaataagg atgaagtcga
     1921 aatcatgctt cagcttatca ataatattct cacgcaatat cgcgtgtttg ttttgtccta
     1981 atagatgctc ctcgcacaga gtatcccagt ttgatgcaat aaaggcgtcg tcaatagaag
     2041 ccggcataac ataaacgcca gggataactg acggaacgat aaaatcttcc agtaattcct
     2101 cacgactcac gttctgcaac atagcctgcg gtgcggttgt atcaaccagc cccaccgcat
     2161 gcaagtagtt cagaaacata gtggctgatg actggggatc taaatcaagg agcagaatac
     2221 gtaaatcttc tgacagcagg tgaggatgag cacgcagggc gtgagctacg gaaacactac
     2281 tcacggtttt agacacaccg ccttttagac taccgatgaa tatggaatat gcctcactat
     2341 aacgatcgcg atatttagga atgccacgat gtgcatagat atcgataatg ttctggatgg
     2401 ttaatgcata ttttgtcgcg gttcctgctg ggcgtttttc aaactgatag ccctgagcct
     2461 ccatctcacc gactgcacca tccacgcttg cgcgcgttaa ttttggaagc ttagctacag
     2521 cagactttga atagacttga taaaaaacat cagcctgaaa ctcctgcttt tgctcctgaa
     2581 tctgttttgt tagggcgagc aacattttat ctgcacgctg cgcaacctta cttaactgct
     2641 caaaactagt cattccccct ccgtgctatg taggttttta acttattaca aaaaaaactg
     2701 cattagcagg gtaatgcatg caagtgcata ttgtacatgc ttatattaaa aatgcgcgca
     2761 ttgctgattt attaatcatc ggaatgaata ttactgtaag atagtatgcc tttccgggag
     2821 aaagacgctg atttcccccg aataatccag ttcaccacca cactccggac aacagctttc
     2881 ttcaggctcc agatggtgta tttcgcggga aacatgttcc ggtaacggac ggcgatgacg
     2941 tgactgtcgc aacaggcggg ggtagtgctt ccaatcagta gaacatgtgc ttttcaatga
     3001 aggagccgat gactttttcg tggatttcca cggaacgcga gaagcagatt gttttacgtg
     3061 ccagccgctt gatgcgggtg cggagggtcc ggttgttacg ttcaatacgt tgcgtgaaga
     3121 ttttgccggt caggtgtttc tccttcggca cttctctggc gtagctgccc cagtcatcgc
     3181 tggtcaccat gccgatacag aacggagtca acagggctaa cagttcccga cag
//
LOCUS       DAJTNN010000267         3217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTNN010000267 DAJTNN010000000
VERSION     DAJTNN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.273"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..1000)
                     /locus_tag="OO150_004027"
     CDS             complement(<1..1000)
                     /locus_tag="OO150_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5517978.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCSLDNTDVNWCSVVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETIINPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GYLGIMTEGENFLCRLLTIEDEVLNVLLRENFNEQLDTNINTIISNLNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETN"
     gene            complement(1262..2852)
                     /locus_tag="OO150_004028"
                     /pseudo
     CDS             complement(1262..2852)
                     /locus_tag="OO150_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT         1032 a          589 c          506 g         1090 t
ORIGIN      
        1 aattcgtttc atgatttcca gctaatacat gaacattttc attgatactc attatgcgtt
       61 ttaacaaatc gatgataaat tgatcgccac agatattact aaatcgatcg ccggtttggt
      121 cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aactaaataa ggctgaagac
      181 tttctaaaac attatctctt ctgttcaggt tactgattat tgtattaata ttagtgtcaa
      241 gttgctcatt aaagttttcc cttagcaata cgttgagaac ttcatcttct atagtaagca
      301 accggcaaag gaaattttct ccttcagtca tgattcccaa atatcccgta gcaaaaagcg
      361 cgtacaaaac tgcaccaact gaaccatcgg tatccccaaa ataggtcggc ctggtacttg
      421 tgggatcaac aggtgaacaa ttggagaaat agattctgtc agcgaactgg gggtttataa
      481 ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc cagatgggtt
      541 tgtacgactc agtaactgca acatcattag cattatcatt tactgtcata ttattaagta
      601 aataaacaac actgcaccaa ttaacatcag tattatcaag agagcaaccg taatttttac
      661 tattaattgt cacaataaac tgataattat tatccctacg tagaatatga gcacgcatgc
      721 ccgatagcat actatatttt tctgaatgat ccgtttgtat ggtaaatgaa attttgtcat
      781 cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc acaacctcac
      841 cgagcattgt cctggcaata cttgatatat catcacctat accatttgca atatacccaa
      901 acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt gcgattgtcg
      961 ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc atattattca
     1021 ccgcgaggaa tacatagtta acattttatc ttatgacata cacctcatgt tcgatggaaa
     1081 aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat ttcttatttc
     1141 atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgtct ctacaatcga
     1201 ttaagaaaac ctatgaaata ctggcaggat taaaaagata taccacagca ctcatggagt
     1261 tttaatgtgg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca cgttcgttag
     1321 gagctatctt ccaggaattt agccccatgg tataattatc aattttttga tgctgatcta
     1381 cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt ccacatttga
     1441 agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattg tcatatacag
     1501 gtgtggatgc tatagcattg cttctaatta gatggataaa acgatcatcc atgtgttgtt
     1561 tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct ttcatctgat
     1621 taaatggaac ctgcaaagca cctagacaat aacattcatt ttcttcattt cttattattc
     1681 cgtgatgact gcttaaaact ttcgtttctg agtcataatt acaaacatcg agcgttttaa
     1741 ttaagttata cgtatcaccg gcagagagtt tatgatttgc tagtcgtgca gtatagttac
     1801 cattgaaatt gatttcataa tttccagcca gaacgacgtc atttttattt atacgactat
     1861 ctttattact ctccatattc cgcatagaat ttagcaaagt aaggatatat ttatcaccaa
     1921 aaatagtact gaatctatcg cctgtatgat ccccaagaaa gatacaaggg gtaatcgcat
     1981 cgccttttaa aaaaacatgt tgatgcaact tattcaagat catgtctctc gtatctaata
     2041 aagtaaagag tttttccaat tttttatttt catatgcgaa tgaagccatg tcttcgtgtt
     2101 ttaataattc acataataaa ctccacccct tttccctgat acctatatgt cctgaaacga
     2161 gcaatgcata aagtacagcg ccaactcttc catctgtatc gccaaaataa gccgtgttgg
     2221 gattttgtgg gttaatcaca ccacactgtt tataaaaatc tatttttgaa aacacagaat
     2281 taacatagct agcaaactta tccttaggaa acgtctcttt agagacatcc caacgctagt
     2341 cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata gaaaggccat
     2401 taactttata aattatgtct gatttatctc cggagaaact aataaaataa taaccatcct
     2461 tacactcaat cgtaacggta atgggagacg acaattttat tacatggtta ttgtctggta
     2521 taattgtaaa gataaacgtg tcagtagtat tatcggcgaa actattatat tcttgaatat
     2581 ctatagatgg ctcattattg taaaaccata tttttttcta tctgagaact atattcattt
     2641 accttccagc gaccatgttg caggaaatag atgatatgca ggcctaaatc gttatctatt
     2701 tgcgaatgtt tatccagctt atcaatttca actatctttg aacgatgttt tttatcaacc
     2761 ttgccatggt gttgatgatt gttaacttga tctgaatgct tatcttgatg gcttgaagag
     2821 atgtgcttaa atatattgtg aaatactttc ataaataacc cgcaatattt ataacttgtt
     2881 agtaacaaaa taaacgatac atgtctgaaa gacatattta cgacgataca aagtaataat
     2941 acttatatat ccgatgattt taagcaccga gactatccgg tcttttacta aataatggaa
     3001 aacaatccat ccctggaaga taattgaaaa aaacaacaga ataagaatta atcttaggat
     3061 aaacatttat ttatcatggc cttttgaacg ctgaaaagta gcgctctgta aagtaaaccc
     3121 cgctatagtt atgctatgca tttgtcttta atgatatctc gtcattgtat taacaggctg
     3181 aaattatcat taatgcacat ttcaatatta gcaggga
//
LOCUS       DAJTNN010000268         3210 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTNN010000268 DAJTNN010000000
VERSION     DAJTNN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.274"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            234..953
                     /locus_tag="OO150_004029"
     CDS             234..953
                     /locus_tag="OO150_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5517979.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVGHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            964..2391
                     /locus_tag="OO150_004030"
     CDS             964..2391
                     /locus_tag="OO150_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5517980.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2384..3079
                     /locus_tag="OO150_004031"
     CDS             2384..3079
                     /locus_tag="OO150_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5517981.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
BASE COUNT          833 a          678 c          911 g          788 t
ORIGIN      
        1 tttcttatgc caaaaatgat cgtcgatttc tccataaaat gtgagcgatg ccgaaagaaa
       61 taaaattagt tattgcattt gacgtttggg tgaaagatta tcatttgtca tacaaatgag
      121 gggtagtatg ttgctagcca attaaaaaag aacgccatat ttattgatga ttgatccccc
      181 ggtggggggg aaacatcggg cataaatggg catgaagtaa tggagtatta gttgtgaatg
      241 tcaatttctt tgtcacctgt attggtgacg ccctgaaatc aagaatggca cgagactccg
      301 tgctgctact ggaaaaactc ggctgtcgcg taaatttccc ggagaaacag ggatgctgcg
      361 gtcagcctgc gatcaatagc ggttatatca aagaagcgat tccagggatg aaaaatctga
      421 tcgccgcact ggaggataac gacgatccca ttatttcacc ggctggctct tgcacctatg
      481 ccgtaaaaag ttacccgacg tatctggcgg atgaacctga atgggcatca cgtgccgaaa
      541 aggttgccgc gcgtatgcag gatctcacct cttttattgt taataaatta ggggtagtcg
      601 atgtaggtgc cagtttgcaa gggagagctg tgtatcaccc atcttgtagc ctggcccgta
      661 agctgggagt gaaggacgag ccacttacgc tgctgaaaaa tgtgcgtgga ctggagctgt
      721 tgacctttgc tgaacaggat acctgctgcg gatttggcgg cacgttctcg gtcaaaatgg
      781 ccgaaatatc cggcgagatg gtgaaagaaa aggttgggca cctgatggaa gtccgccctg
      841 agtatttaat tggtgctgac gtgagttgcc tgctgaacat cagtgggcga ttacaacggg
      901 aagggcagaa agtcaaagtg atgcatattg ctgaagtgtt gatgagccgc tgaggatata
      961 aagatgtcga tcaaaaccag taatacagat tttaagactc gcatccgtca gcaaattgaa
     1021 gatccgatca tgcgcaaagc ggtggcaaac gcgcagcagc gtattggggc aaatcggcaa
     1081 aaaatggtcg atgaattggg gcactgggag gagtggcgcg atcgggccgc ccagatacgt
     1141 gatcatgttc tgagtaatct cgacgcttat ctgtaccagc tctcagaaaa agtgacgcaa
     1201 aacggcggtc acgtctggtt tgcaaaaaac aaagaagacg ctacccgcta cattttacag
     1261 gttgcccaac gcaaaaatgc ccggaaggtg gtgaaatcta aatcgatggt gaccgaagag
     1321 attggtgtca atcatgtgtt gcaggatgct ggcattcagg tgattgaaac cgatctgggt
     1381 gaatatattc tccagctgga tcaagatccg ccatctcatg ttgtggtccc ggcaattcat
     1441 aaagatcgcc atcagatccg tcgagtgcta cacgaacgtc tgggctatga ggggccggaa
     1501 acgcctgaag cgatgacctt attcatccgg caaaaaatcc gcgaagattt cctcagtgct
     1561 gaaataggta ttaccggctg taatttcgcg gtggcagaga caggttcggt atgcctggtg
     1621 accaatgaag gtaatgcgcg aatgtgtacc acgctgccta aaacgcatat tgcagtgatg
     1681 ggaatggagc gtattgcccc cacgtttgcc gaggtagatg tattgatcac catgctggcg
     1741 cgcagtgccg ttggtgcacg tttgacggga tacaacacct ggctgacagg accgcgcgaa
     1801 gcggggcacg ttgatggtcc tgaagagttt catctggtta ttgtcgataa cgggcgttct
     1861 gaggtgctgg cctctgaatt tcgggatgtg ctgcgctgta ttcgctgcgg ggcttgtatg
     1921 aatacttgtc cggcatatcg ccatattggc ggtcatggat atggctctat ttatccaggg
     1981 ccaattggtg cggtgatttc tccgctactt ggcggctata aagattttaa agatttaccc
     2041 tacgcctgct ctttatgcac tgcttgtgac agcgtgtgtc cggtgcgtat tccgctgtca
     2101 aaactgattt tgcgtcatcg tcgggtgatg gctgaaaaag ggatcaccgc aaaagcagag
     2161 caacgggcga taaaaatgtt cgcttatgcc aatagtcatc caggattgtg gaaagtcggg
     2221 atgatggccg gcgctcatgc ggcaagctgg tttatcaatg gcggcaaaac accactcaaa
     2281 tttggcgcga ttagcgactg gatggaagca cgcgatcttc ctgaagctga cggagagagt
     2341 ttccgtagtt ggtttaagaa acatcaggcg caggagaaaa agaatggata atcgaggcga
     2401 atttttgaat aacgttgctc aggcactggg tcgcccgctg cgacttgaac cgcaagcaga
     2461 agatgcgccg cttaacaact atgctaacga gcggcttacc caacttaacc aacagcagcg
     2521 ctgtgacgcg tttattcagt ttgccagcga tgttatgttg acgcgctgtg agctgaccag
     2581 cgaggcgaag gcggcagaag ctgcaatacg tctgtgtaaa gagctgggag atcagtcggt
     2641 cgtgattagc ggtgacacga ggctggagga attggggatt agtgaacgtt tgcagcagga
     2701 atgcaatgcc gttgtttggg atccggcgaa aggtgccgag aatatctcgc aggcagagca
     2761 ggctaaagtg ggtgttgtgt atgctgaata tggtttaacc gaatcgggag gcgtggttct
     2821 tttttccgcc gccgagcgcg ggcgttcatt gagcctgctc ccggaatctt ctctttttat
     2881 cctgcgtaaa agcacaatcc tgccgcgtgt agcgcaactc gcagaaaaat tgcatcagaa
     2941 agcgcaggcc ggtgaacgaa tgccgtcctg cattaacatc attagcggcc ctagttcaac
     3001 ggcggatatt gagcttatca aagtcgtcgg ggttcatggc ccggtgaaag cggtgtatct
     3061 gattattgag gattgctgag agagatatga gatctgaaca taaacctgaa gaaggcatac
     3121 gaggttgaaa atagcgttaa ggacaacctg acttaacccc gttccgtcat ggttatgctg
     3181 ctcttctgta aatatcctgg cattagtaat
//
LOCUS       DAJTNN010000269         3208 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTNN010000269 DAJTNN010000000
VERSION     DAJTNN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.275"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..213)
                     /locus_tag="OO150_004032"
     CDS             complement(<1..213)
                     /locus_tag="OO150_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517982.1"
                     /translation="MIRFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVCISLQELQLELMVEYALFFS"
     gene            complement(216..983)
                     /locus_tag="OO150_004033"
     CDS             complement(216..983)
                     /locus_tag="OO150_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517983.1"
                     /translation="MQPCPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESLGK"
     gene            complement(986..1696)
                     /locus_tag="OO150_004034"
     CDS             complement(986..1696)
                     /locus_tag="OO150_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517984.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1712..3208)
                     /locus_tag="OO150_004035"
                     /pseudo
     CDS             complement(1712..3208)
                     /locus_tag="OO150_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          911 a          770 c          707 g          820 t
ORIGIN      
        1 actgaaaaaa agtgcatatt caaccattaa ctcaagttgt aattcctgaa gagaaataca
       61 cacaaccagg agcggaaagg agaatattat ttctgccaga aaagttaacg tgaatataag
      121 tacggctaag acaataaccg tcggccatac caataatggc acgataacaa aaccgagaat
      181 tacaggggga atataaagcc ggaatctaat catttttatt ttcctagtga ttcggatgat
      241 gcgtcataca tcgcgcataa acactcatca attatccagt catcttccca gacctgatat
      301 tctgtcattg gttcgggctg ttttagcggg ttctcaatcg cttgccgcgc tctttctttc
      361 agtaaaggat gattatttct agcccaggca ttgatttggg tggtaatgac gttatcgata
      421 tcgtcctggt tatctctgat ataatcctca caatatctgt tcaataaaca ggcaagagaa
      481 acggtcgacc aaaagccggg aactgaatta atggcatcca gcaatggcgc aagagcgata
      541 tcggcacgga aaaacatttc cagcatatac tgtaattcga cagcttcatc gccggatacc
      601 caatcagtca tatactggca ggcaaatcgc tgtaaaattg cccgttcatc cgcacgccag
      661 ttttctttgt tggcaaggtt gatacggcgc agagaaaaaa ctacgtgaat attgtcaaaa
      721 tgcccctgtg caacaaattc cagtaaacgc ggcagaaaat agcggacctc atcgctattt
      781 tgcggatcag gccctgggct actattccag gcattgatta aggaatacgg gatcgctcgt
      841 aacgatgtac tccgtaatgc cttttgttcc tgctgactca ggcaatattc acaacaaacc
      901 acaaatttct gcggcacccg ataacgacga aaggtgtgat aaatctgctc aattaatgcc
      961 tgcatggcgg ggcagggttg catagttacc ccctcgcccg ctgatcgcgt gtaaacggtt
     1021 gatagacaaa acgagcattc gccgcgttca ggcgaataag ggcgctacgc gaaagggaaa
     1081 agctggggcg aatgcaacta ttgcagtctc gacagctttc ctctgcgcaa ttttccactg
     1141 agtaatgacg cgtgtagagt ttcagttcgg cctgcttatc gcgaaatccg cgtaaactgt
     1201 tattgttatg aaatttccag gcatctttaa agacctgaaa gatatagaca tcctcaatcc
     1261 aggagtcaca ttttttaagc gggcgctttg ctattatcac ccgctcatcc ggatttagca
     1321 tcgccgtaaa ttgtacggtg acgtcatttg tcgtgttcag gtcacattga acatctggta
     1381 catcactgat ataggggtca aatccaaaat cagcaccaca ggcacaaaga cggctaatgg
     1441 tggcggtatc gagggaaaat cccggccata ctttgctgga ggttaagcca atatttaatg
     1501 tgacatcata tcgaagcaat ccctcaagac cgtcagcacc gacgaacttt tccccttcat
     1561 ccagggcctg gttaataagt tgctgaacaa tgccttcatc ccagtatgcc gatttgcagt
     1621 tggcaatcgt aatatccacg cctgtgaagg gatcagtacc ggcaaggctt gcaaataatg
     1681 tattaactaa ttccattttt attatccagc tttaaggtag cgtcttatac atcgggacat
     1741 aacctacatc ttccaccaaa ctctgtccct gcggcgttaa aaaccactcc aacaattttt
     1801 gtgtctctga cgtcgtattt tcccgcgtta ccataaaggc atcgatgata tagggatatt
     1861 tgccgttgcg aatgttttcg gcagtcggtg caataccgtt aatcgctagc agttttatat
     1921 ttttatcggc gttcatttgt gttgcgtaat aacggaaggt gtaacctatt gcgttgtttg
     1981 tattacggta ttcggcaacg actttaatca ttccctccat catacttgcc acttctgttt
     2041 cctgcggcga gatcatgcgg acatttttca tgacctggga ttgcatcacc gtctggctac
     2101 cagagtcttc cgggcgttgc taggtctgga tctcctgatc gttaccgcca acagtacgcc
     2161 agttggtaat tgcaccactg aagatgtcac gcacctgttg ttcagtcagg gaattaaccg
     2221 gattatccgc attgacgatg aaaacaaacg cttcacgggc aaaaggcgtg tacagcaaag
     2281 taatgcccga ttcctcagcg cgttttttct gcccaccgga aggttgcgcc acaaaaataa
     2341 tatcagcatc acctttaaca atcctgttat acgcatcggg ggtacgagag ctctccagat
     2401 attccctggt gtgaaaatct tccggtataa cgctcaaagc ataaaatgca gaggcataaa
     2461 ttgggtacgc cgccgtagca ccatcgagtc gcggccagtt ttgcgtaaac tgaatttgcg
     2521 gtttaccacg cagtggtgtc agttgattat tgagtttatc gggtcgccag gcccaggtat
     2581 caatattttc gcgcacaccg acgccgggat tgagctgttt atataaataa ccctgataac
     2641 cagcgacaaa accaagcatg agaaggataa acaggaggga attacggcaa cgtagcccgg
     2701 atgtaccggt taaaggatgt ctggttggcc ggccgtaccc cagcgaaaaa ctgatatgcg
     2761 cgccaacggg tataatgact atccaccagt actcacctcc gactactgag actaacaaaa
     2821 cgcccagcca ggggagcata agatcagcca accaacccga tcccatctgg tgaaattgat
     2881 aatctgccag atgcagcgcg attatccagc caaccaggat taataaaccg ggtactaaaa
     2941 aaagacattg tcgccagacg gctatttctc ttttttctgg tgcactatcg cctgcccata
     3001 ctcgccccga gaaaaatagc attaatgcgc ccagcgccag cataaaaatg ttgagcagaa
     3061 ggtaacttgt tggcgtgata aggatattac aaacttcaat aataccggca agtccgacac
     3121 ccagcatagc aacaaccgcc gccaaaaatt gcgtcagtat ggggatgccg aaaaaagtca
     3181 ttaccagcga ggacaaaatc cattttct
//
LOCUS       DAJTNN010000270         3206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTNN010000270 DAJTNN010000000
VERSION     DAJTNN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.276"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(94..513)
                     /locus_tag="OO150_004036"
     CDS             complement(94..513)
                     /locus_tag="OO150_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5517985.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            593..847
                     /locus_tag="OO150_004037"
     CDS             593..847
                     /locus_tag="OO150_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5517986.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            844..1269
                     /locus_tag="OO150_004038"
     CDS             844..1269
                     /locus_tag="OO150_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5517987.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDALKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            1341..2411
                     /locus_tag="OO150_004039"
     CDS             1341..2411
                     /locus_tag="OO150_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR5517988.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPVGTTPEADSAYALKADSGAVQQAMTASPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            2452..2874
                     /locus_tag="OO150_004040"
     CDS             2452..2874
                     /locus_tag="OO150_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5517989.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRILSFYQGNFQEVMA"
     gene            2871..3176
                     /locus_tag="OO150_004041"
     CDS             2871..3176
                     /locus_tag="OO150_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCR5517990.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
BASE COUNT          875 a          696 c          874 g          761 t
ORIGIN      
        1 aaagtgctta tggtacattt tgtgtgtaaa aaaatgagat gggataaagt gaagcacaaa
       61 cccggaggaa ggcgctaccg gatttatgct ggtttaagag gctttttgtt ttttctttcg
      121 tgctaactct tcgtaaattg cattgtactt ctgttttttc tcttcaagag tttttaaaag
      181 ttcatctgtc tcactgtcag ggagctcgtc cagaaggtca atgatgattt tttgtcttgg
      241 atttaactcc tgatagaaac gtacctgtcc actttcttct gtatcctctc ccaaaagata
      301 ggttggtgtt gttcctatta gtgttgctaa ttcccttaat ttctcccggc gaggaattgt
      361 ttcgccatta aaccatttgc taaccgcttt tggtgttaat ttcattcgac gggcaatttc
      421 tgcctgcctt ccatgttgtt cataaccagc gttttcacag gctagcgcaa gcctactggc
      481 gaactcttta cgcgctttat cttcatgaac cataagttca atgatattcg ctcttgaatg
      541 tactgtcagt tctgttatag catgtactca aagttcacat tgtgagggtg atatgaacca
      601 gaaaacactt gaagatgtaa tcaaaactgt tcgcgttgct gttgtggccg acgtttgtgg
      661 tgtcagccaa agagcaatct ataaatggat ggataacgga aaattgcctc gcacagaata
      721 taccggcgaa acaaattacg ctgaaaaaat cgctcttgca tcaaacggat tattttctgc
      781 cgatgcaatt ttaactattg gcaggaataa aactactacg aaaaagctga tgggagttga
      841 ttcatgaaaa tcaagcatga acacatccgc atggcgatga atgcctgggc gcatccggac
      901 ggcgaaaaag taccggctgc gaaaattacc aaagcgtatt tcgagctggg aatgacgttc
      961 ccggaactgt atgacgacag ccatccggaa gccctggctc gcaatactca gaaaattttc
     1021 cgctgggtgg agaaagacac ccctgatgcg cttaaaaaaa ttcaggcgtt gttaccagct
     1081 atcgaaaaag caatgccacc tctgctggtg gcccgaatgc gcagccatag ctcagcctat
     1141 tttcgggaac tagtggagac gcgggaacga ctggtgagag acgctgatga ttttgtcgca
     1201 gtggcgatcg ctggtttcaa ccagatgaat cgtggtggcc ctgcaggaaa tattgtggct
     1261 gtgcattgac tcgcaatatt cataccggat cacttccggc aatttgtgag taaaaagatt
     1321 cggtatcaaa agaggtgagt atggctaacg cctggctcag attatggcat gacatgccaa
     1381 atgaccctaa gtggcgaaca attgccaggg tgtcagggca gccaattgca acagtgatgg
     1441 cagtgtatat ccacctcctg gtgagcgcgt cacgaaatgt cacgcgaggt cacattgatg
     1501 tcacgacaga agatttggca agtgcgctcg acgtgacaga agaggtaatt gattcaattt
     1561 tgcagacgat gcaggggcgg gtacttgatg gtgatttaat cactggatgg gaaaaacgcc
     1621 aggtgctgaa agaggacaac ggcaatattt cgcaaaccgc aaaatctcct gcagagcgca
     1681 agagggcgca gcgagagagg gaaagaaagc gggaacaaaa tggcgattgt cacggcgcgt
     1741 cacgatatgt cacgcacatg tcacgacgag tcacgacaga taaagataca gataaagata
     1801 cagatcaaga agatcaaaac actatggtcc atggcgtaaa aaacgccacg aaccaggcag
     1861 gggatgttca gaccgtcaat cctggtcagc cagtaggcac gacaccggaa gccgattcag
     1921 cgtatgcgct gaaagccgat tcgggcgctg tgcagcaggc gatgaccgca agtccggagc
     1981 aatcacacca actgcagcag cctgaagccg attccgccat tcagcgggaa gccgatcggg
     2041 tagtcccgga aaacaccggg cagtctgtgg gacgagtgga ttatccggat gtgttcgaac
     2101 aggtctggcg ggagtacccg ttgcgtgccg gggcaaaccc gaagaaatcc gctttcagtg
     2161 cctggaaggc cagattacgc gagggggtgc caccagaggc catgctggat ggcgtgaggc
     2221 gttacgcaag atacctggcg gctaccggga aaacgggaac ggaatttgtt cagcgagcga
     2281 cgacgttttt tggaccggac cggaattttg agaacccctg gttgctcccg gtaagcggca
     2341 cgaacaacca gcgttgtgtg aatcatattt ctgaaccgga taacgaaatt ccgccgggct
     2401 tcagggggta agtgttaatt tctggtcatg aggtaatttt caggagggct tgtggcaaaa
     2461 gtttttacac aagaagagcg ggaaaaaatt aaagggcagg ttcttgaact cgtacgccag
     2521 agtgggcgcg agacgttacg acaactggaa gctaaaactg gggcaacaag atatctgatg
     2581 agcgttctgg ccagagagct ggttgccagt ggcgatgtat acaactctgg ttacgggtta
     2641 ttcccgtctg aacaggcgcg taaggactgg caaaatgccc gtaaaaagct ctcaagggca
     2701 aagctgaaga aaccatctgc ggttgatccg gaccttatct ggtcattacc tgacggagaa
     2761 atacgtcgct acgacagtcg cctaaacata atctgtcgcg agtgccggaa gagcgaagtt
     2821 atgcagcgca tattgtcgtt ttatcagggt aattttcagg aggtgatggc gtgagggtga
     2881 gagtctatat cgccggtcca atgaccgggt ataaaaattt caaccgtgag gcgtttcaca
     2941 atgcggaaga ggaactgaaa cgggaagggc ataccgtctt aaacccggca gtacttccgg
     3001 acgggctgac acagccgcag tacatggata tctgcatggc gatgattcgt tgtgtggatg
     3061 cgatttacat gctgaaaggc tggcagcggt cagcaggcgc taaggcagaa ctggcgctgg
     3121 cggagaaact ggggcatgcg gtgatttttc aggagacagc cagtgtgcaa gactaattac
     3181 caggcattac gtgaacgtta ttcacc
//
LOCUS       DAJTNN010000271         3180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTNN010000271 DAJTNN010000000
VERSION     DAJTNN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.277"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            189..929
                     /locus_tag="OO150_004042"
     CDS             189..929
                     /locus_tag="OO150_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5517991.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(900..1667)
                     /locus_tag="OO150_004043"
     CDS             complement(900..1667)
                     /locus_tag="OO150_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5517992.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1873..2451)
                     /gene="lpcA"
                     /locus_tag="OO150_004044"
     CDS             complement(1873..2451)
                     /gene="lpcA"
                     /locus_tag="OO150_004044"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5517993.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2691..>3180
                     /gene="fadE"
                     /locus_tag="OO150_004045"
     CDS             2691..>3180
                     /gene="fadE"
                     /locus_tag="OO150_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="functions in fatty acid oxidation; converts
                     acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR5517994.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHR"
BASE COUNT          761 a          807 c          817 g          795 t
ORIGIN      
        1 ataaatgata catgcgcttt tcggactatt gaaaactgcc cctgtgctaa atttgtaaac
       61 cctgcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt ccaaatccag
      121 actattccga tttgggatcc attttgccgt cctggcgtgt gtaaccgttt tatcaaggaa
      181 taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg tgcgtttcgt
      241 ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag tataagcagc
      301 agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg ctcgatctct
      361 acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta taaaatctgt aaatattccg
      421 gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg ttttatagcg
      481 tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat attggtttcc
      541 ccaatgccta tgaccgtgcg catggttatg aagggaaata cctgatgatc cacggcgatt
      601 gtgtttccat cggctgctac gcaatgacca atcagggtat tgatgagatc ttccagttcg
      661 ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt gagtatttac ccgttccgca
      721 tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc tgggagcaac
      781 tgaagccggg ttacgactac tttgagcaaa cccgtaagcc accgaccgtt tctgtggtca
      841 atggtcgtta cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca caactggcat
      901 caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat cttttgccag
      961 gtttcattgc ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt cggtgtggtc
     1021 tgcgagctga agtcgatttc tacatcctga tccagcagcg ttgccacgcc aaacggtgcg
     1081 cggcgggtga tccagtgcag attcgtcgag caatacgcca ttacatagcg cccatccgaa
     1141 agcagcatgt tgaaaacgcc cttctgccgc agttcgtccg ccagtgaagc gatatattta
     1201 aacactgctg ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt atgcagcagc
     1261 cagcagaagg ctttttcgct gtcggtttcg cctaccgggc ggaagttgcc ggtttccagt
     1321 gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc ccataactcg
     1381 cgggtaaatg ggtgagtatt ttccaacgcc acctcgcccc gattagcctg gcgaatatga
     1441 gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat gggggaatta
     1501 aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa ggtaatgccc
     1561 cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt gaaactaaag
     1621 cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg taacctccac
     1681 aacagcggga gcgcatggcc cccggcgaag attgatttaa gaattttcag gtcggatgcg
     1741 gcgtaaacga attattcggc ctacggttcg cgtacagatt tgtaggcatg ataagacgcg
     1801 tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg ccttatccgg
     1861 cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga tatggatcac
     1921 tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc gaatttcgat
     1981 atccgccgtg ccagccattt tgccgccgtc tttaccggtc agggtgatca ctttcatccc
     2041 cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg aggtggagat
     2101 ccccagcagt acatcgcctt cgcgacctac cgcttcaacg tagcgggaga aaatatcatt
     2161 gaaaccgaaa tcattaccga cgcaggaaat atgactaacg tcagaaatag caatcgccgg
     2221 gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt gcatagcgtc
     2281 gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa agctgtctgc
     2341 taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta aaaagttagc
     2401 cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcttggtaca tgaggatatc
     2461 cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa agcgagaagt
     2521 acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa cagcaacaat
     2581 gtgagctttg ttgtaattat attgtaaata cattgctaaa tgtatttaca tccactacaa
     2641 ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt atgatgattt
     2701 tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat
     2761 ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct ggtctgtggt
     2821 cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt gcgcctatgc
     2881 gtaagtcgat gatttccgcg ccggtatttc gaggtttccg taaggtgatg ccgccgatgt
     2941 cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc gacttgttcc
     3001 agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg accgccgaag
     3061 agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat gatttccaga
     3121 ttactcatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa gagcatcgct
//
LOCUS       DAJTNN010000272         3163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTNN010000272 DAJTNN010000000
VERSION     DAJTNN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.278"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            336..495
                     /locus_tag="OO150_004046"
                     /pseudo
     CDS             336..495
                     /locus_tag="OO150_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(713..2803)
                     /gene="iha"
                     /locus_tag="OO150_004047"
     CDS             complement(713..2803)
                     /gene="iha"
                     /locus_tag="OO150_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HCR5517995.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          793 a          832 c          731 g          807 t
ORIGIN      
        1 gtaaatatca atacggttct ggcgagccgc ttacgtgcag cagtcttatc cggcagttta
       61 aaggggagga cggagaccaa tgaggcactg gagcagattc agtgcagagg tgacctgctt
      121 tatcaaactt aattgtgaca aaatggcgat tgagaaggtg caattgactt atccggattc
      181 ggtcgtggcg aggtggtgcc cctgcattct gctaaccggg ttatgagcca ggagtcagcg
      241 atagacaaag ggacttatct gaccgggccc ccggaaaaat ggcgggagag aatgatacct
      301 tcctgctgac gagaccggac tttgagatga cgagcgatag tgcggcagtg atgtacagcc
      361 tgatcggtag ctgcaaaact taacggaatc gagccggaaa cgtggctacg ccacgtgatc
      421 agtgtaatca acacctggcc tgccaaccgc atgaaagagt tgttgccctg gaacgtcact
      481 ctctctgtaa actaattttt accccacgtc cttcacggtg cgcttacact ctggacaagt
      541 atgtaaatga taagttaaaa ataatccgtg catttcctgt gacgatatta gttgttggta
      601 acgaaatctc attagcggat cggaaatgca cttaacctaa tacgttgcca ctgttccgcc
      661 aggagttgca gtggcaacgt attctaccgt cagtgatagc gttttgttat tatcagaact
      721 gatagttcag cgacatccag taatttcgct caggtatcac atatcctgtt gttgatgatc
      781 ccgtctggaa gtaatcaccg gcatacagcg tactcttacc ggcactgtac aggctcacgt
      841 cactgtaatc cttgttgagc aggttattca ccgcagcatt cagcgtcagg gcatccgtca
      901 tcttccagga cagacctgca tccaccaccg tccaggcttt caggtattct cctttctcat
      961 catacacttt cttctgtaca gcgctcagtg acgaataatt ctgggtgaaa cgtggtgttt
     1021 tcccgcgata acgggcaccc agccatgatg ccacctcttc ggtgatctgc cagttcagtt
     1081 tcgcattcac catgtgttca ggggtataac tcagcggcgc acctttgtta tcaccatcac
     1141 gttgttcact tcgggtccag gtgtaattca gtgacagcgt gacatcctct gaccacagcg
     1201 gcaatgtgcc ggcaaattcc acaccgtgca accgggcctt tccgctgttt acatagctat
     1261 tggtgttatc atttatggaa taagagacaa tcttgttgga gaagtcagtc ataaaacctg
     1321 tgacattggc attcagaccg gcggggttat cgtaatacac cccagcctca taactgacgc
     1381 tctcttccgg cttcaggtcg gggttaccaa gtagatttgt ttttccctgc ccggacacac
     1441 cactaatccc tttatgtagc tgccccattc tgggtgcctt atatcccgtg gtcacaccgc
     1501 ctttcagcgt ccaggcatct gccacatccc agaccagata tgcacgcgga ctgaagtgtc
     1561 ccccgaattg ctcatgatgt tcatagcggc tgcccgcagt cagcgcaagt gcatccgtga
     1621 gatgccactc atcctcagca aataccgacc agcttttctg ccggaaagtt tcacctgtgc
     1681 tggcaaggac aactccgtct ttcatggacg agctctgaaa ctcgcccccc accgtaacca
     1741 gatgagattc tcccagaggg gtaagcagta atgaattcag gataaggttc gattccttaa
     1801 gctcccgcgg ctgaccggca agcccccatt tgtcgcgctt cagtacactg cgtacgagct
     1861 cacgaccttt attttctgtc tcgttccagt tcagatacga tttccatgtt ccgaaggtga
     1921 aagtatgatc atagccagct gaaattttgt tccgctcata gcgcagggtc cggtcatatc
     1981 cccccgtcag actccccagt tgcccatccc ggttatcata acgctgccgg gtggtatcca
     2041 tatcaaacca gagcacatcc tgctccgacg ccttccagtc aagacgtgca ccaagattat
     2101 aattctgtga ctccgtggga taaggaatac gcgtggctgc tgtatcgctc agtgatgtga
     2161 ccgatgagcc ctgacgctgt tgtgtgctac cgcgtacctg caggctgaca gaatcatcca
     2221 caagggggcc actgctccag aaattaaact ggctgctgtt accccatttg ttgctttcct
     2281 gcagattcag ccctgcattg acggaagaga gccatttgtc tgcattcttt ctggtaatga
     2341 tattcaccac accgcccatc gcatcagagc catacagtgt ggacatcggc cccctgataa
     2401 cctcaatacg ctcaatggcg gccagagggg gcatgaaccc ggtattcatg gcagaaaaac
     2461 cgttgggagt cacgtcactg cttccgccct gacgaacacc atcaatcagt atcagcgtgt
     2521 aactggctgg cattcctcgg atgctgattt ccagcccacc ggttttaccc gtaccacttt
     2581 caacatccac accctctact gatctcagag cctccgccag atcgtggtac tggctggact
     2641 gcaattcctc ctggctaatc acagaaacac tggcggctgc gttagtcagc tttttctcat
     2701 agccggatgc cgagacaatc atcacatcct ctgcagcagc tatgctgctg gctgaaaatc
     2761 cgagacaggg aatgactacg gaagccagag tggttattcg catattacta atctccgatc
     2821 atgttaaccg gcaagacaat acacaaatac aaacaataat gagaataatt tatatttgcc
     2881 ttttacaaaa aataaagatt attaacaaaa ctaaatataa ataacaactg cctcggcatg
     2941 ccagttaaca gctatccgta tggcgtgttc ggttttgcga ataaaaaatg gttactgtag
     3001 aagcggattt ccaccacatg tcaaccatga atggtcagtg gtgcgagacg actgggatga
     3061 tttggtcgat gacttcttcc ggtgaatact gatagaaaca ttgaaatatt gtaagagaaa
     3121 gcgttcaaaa ttctgtggca cgtgaaaaat tctacagcgt cat
//
LOCUS       DAJTNN010000273         3145 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTNN010000273 DAJTNN010000000
VERSION     DAJTNN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.279"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..142
                     /locus_tag="OO150_004048"
     CDS             <1..142
                     /locus_tag="OO150_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="activator of the allDC-ylbA operon involved in
                     allantoin utilization; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5517996.1"
                     /translation="MRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNP
                     RS"
     gene            211..1305
                     /gene="mnmH"
                     /locus_tag="OO150_004049"
     CDS             211..1305
                     /gene="mnmH"
                     /locus_tag="OO150_004049"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5517997.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            1421..1786
                     /locus_tag="OO150_004050"
                     /pseudo
     CDS             1421..1786
                     /locus_tag="OO150_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2348..2797)
                     /locus_tag="OO150_004051"
     CDS             complement(2348..2797)
                     /locus_tag="OO150_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5517998.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFSHVAVDISIYTNVEDDYFFSYFSLTNDDGGRFKKTLTVR
                     AREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFLLVKLMLRIIL"
     gene            complement(2778..3145)
                     /locus_tag="OO150_004052"
                     /pseudo
     CDS             complement(2778..3145)
                     /locus_tag="OO150_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          841 a          727 c          713 g          864 t
ORIGIN      
        1 gatgcgccct ccttcgccat tgagtctggc atggcgcaaa tttggcagcg gcaaagcggt
       61 agaagatatt gtgaccttgt ttacccagcg caggccggaa atcagcggat ttttagaaat
      121 tttcggcaac ccacgcagtt aaaaatcaag tttccttgcg cgttggcgcg taaaatagcg
      181 cgcaagctga tttgaggttc acttttaact atgcaagaga gacacacgga acaggactat
      241 cgtgccctgc tgattgctga tacgcccatt attgatgttc gcgcccctat cgagtttgag
      301 caaggcgcaa tgcccgccgc tatcaatctg ccgttaatga ataacgatga acgcgccgcc
      361 gttggcacct gctataaaca gcaagggtca gacgcggcgc tggcgctggg gcataaactg
      421 gtggcgggtg aaattcgtca gcagcgcata gacgcctggc gggcagcgtg cctgcaaaat
      481 ccgcaaggta ttctctgctg tgcccgtggc ggtcaacgct cacatattgt gcaaagctgg
      541 ttgcatgcag cggggattga ttatccgctg gtggaaggcg gttataaggc actgcgccag
      601 accgcgattc aggcgactat tgaactggca caaaaaccga tagtgctgat tggcggttgt
      661 accggcagcg gtaaaacgct gttagtgcag caacagccga acggtgtcga tctggaaggg
      721 ctggcgcgtc atcgcggttc ggcgtttggt cgcacgttac aaccacaact tagccaggcg
      781 agttttgaaa atctgctggc tgccgaaatg ctaaaaaccg acgcccgtca ggatttgcgc
      841 ctgtgggtgc tggaagatga aagccggatg atcggttcga atcacctgcc ggaatgtctg
      901 cgcgagcgaa tgactcaggc ggcgattgcg gtggtagaag atccgtttga gatccgtctt
      961 gaacgcctga acgaagagta tttcttgcgt atgcatcatg attttaccca cgcgtacggc
     1021 gacgaacagg gctggcagga atattgcgaa tacctgcatc acggactttc ggctattaag
     1081 cgtcggctgg ggttacagcg ctataacgaa ctcgctgccc agctggatac ggcgctgaca
     1141 acgcaactca ccaccggcag caccgacggt catctggcct ggctggtgcc gttacttaaa
     1201 gaatattacg acccaatgta tcgctatcag cttgagaaaa aagcggaaaa agttgtcttt
     1261 cgcggtgagt gggcggaagt ggcggaatgg gttaaggcgc ggtaatgagg gacgtatttg
     1321 tattagtcgc gacttgatct gacacatggc cttgaaaggt tgagagttac cggttttgat
     1381 atgagtgtcg aatccttatc caaaacaaga ggtaactctc atgcttcata ctgccaatcc
     1441 cgtcatcaaa cacaaagccg gtttgctcaa tctggctgaa gaactcagca acgtatcaaa
     1501 agcctgtaaa atcatgggcg tttcgcgtga tacgttttat cgttaccgcg aactggtcgc
     1561 tgaaggcggt gtggatgcgc agattaatcg tagtcgccgc gctcctaacc ttaagaaccg
     1621 taccgatgag gcaactgaac aggctgttgt tgattacgcc gtcgctttcc cgacacatgg
     1681 tcagcaccgg gccagcaacg agctgcgtaa acaggggggt tttatctccg acagtggtgt
     1741 ccgttctgtc tggttactcc ataaccttga gaatctcaaa agacgatact gaacaaattt
     1801 tatcaggtga cgttccgtaa aaagttgtat ggagttctcg atgcagtcgg cgcgactatc
     1861 agtacccccg ctgaaccctg gactatcggg ccgccgtact ggggcatgtc accctggcgt
     1921 gccgctggtt ttccgctaat ggtttatttc ctctttcttt cccggcaaga atttatttta
     1981 atccggaagg attatttatt ttcagatagg gactcacatt aaacacgtaa cattttaatt
     2041 aatgcatggc tgaatatgta ttacacaatg tcagatatcg taaaagacca gtgcaattct
     2101 atgtaaactc tctgactgtt cattttattt gttgtttcag ggtcggaact caccctcaat
     2161 aaacaactga atacgagtag atattcatcg tctgagctat atggctttac acaatagccg
     2221 acatgtcttt tctcagtcag gaattttact tttcctcact acgaagttga cgacttcgat
     2281 atgggataga ctcttaattc aagcaattac tctgcacaag catcaggcaa tccagctcca
     2341 atatttgtta taaaataatg cgtaacatta atttcaccaa caaaaaaagg tttacaccat
     2401 ccccttgccc tggtatctta ccatatttac accacacttt ttttgtatct ggatcattct
     2461 ttgatacgat tttcgccact tgttccctgg ccctcactgt caatgttttc ttaaacctac
     2521 ccccatcatc atttgttagg gaaaaataag aaaaaaaata atcatcttct acatttgtgt
     2581 atattgatat atccaccgct acgtgcgaaa aaaaatccgg gagccatgta aaaactccat
     2641 cattttctgg tattgccata tagtttatgt aatgtttact tacaagagat tcatctttgt
     2701 ataaataaag ataagtaatt acgcataaaa caacaatgat tataatagca aaaataaata
     2761 ttatcatctt tgatagatta cttgagatag ccagcatctt gtaaagcctt tatcgttttt
     2821 ttatgctctg gattaatata atcactacat ctatctgagc aatctgttgt tgatggacat
     2881 gtcaacccat ggtcatttac agccaagtct tttttattat cttcaatcat ttcagaatgg
     2941 gatagcttta cttttctgcc gtacataccg aacagattta atccgtaatc tctaatctct
     3001 ttttcataac ccagcccttt cgccgatcgg ctaacaccag cgtcatttaa tttagacact
     3061 cgacaaaatg ccatacaatg gaaaaattga tcagtcccac ctaaatttaa tctcttcata
     3121 agtatcagat aatatatccc atgct
//
LOCUS       DAJTNN010000274         3127 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTNN010000274 DAJTNN010000000
VERSION     DAJTNN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.280"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            600..884
                     /locus_tag="OO150_004053"
     CDS             600..884
                     /locus_tag="OO150_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5517999.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            872..1357
                     /locus_tag="OO150_004054"
     CDS             872..1357
                     /locus_tag="OO150_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5518000.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            1391..2569
                     /gene="ltrA"
                     /locus_tag="OO150_004055"
     CDS             1391..2569
                     /gene="ltrA"
                     /locus_tag="OO150_004055"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5518001.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNTIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2652..2806
                     /locus_tag="OO150_004056"
                     /pseudo
     CDS             2652..2806
                     /locus_tag="OO150_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2860..3127)
                     /locus_tag="OO150_004057"
                     /pseudo
     CDS             complement(2860..3127)
                     /locus_tag="OO150_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          824 a          681 c          840 g          782 t
ORIGIN      
        1 ggcggcatcc ggcatatagg cagcatgaac gcaacacagg ctctcgtacg gaacgtggga
       61 acctgtcgtc tcgatgctaa gggagcggcg cggagcagag gcactgcaag cgcctgagta
      121 ccgatgcgag acacaggggc agatggcttc gtagtagtga ggaagtgcct gtaatgggca
      181 tggagcgaag ggggtcagtc gaggcgtttg aggttaccaa tcgaagctat acccggtaat
      241 ggcgcaggtt gacctgcact tggccaagtg gattgcccgc aaacacaagc gagcgcgagg
      301 tagtctggtg cgtgcattcg agtggttact gcgaattcgt cacgactgcc cgacgctgtt
      361 tgcacactgg tgcttagctt acgatacttg aatggttcaa cgacaagagc cgtatgacgc
      421 gagagtgtca cgtacggttc cgtgggagcg taggggggcg gtgcccctgc gccacccgat
      481 atcccctttg agtgcggtta cgtccacaga ggtaaatgcc gctgacgcgg catctgtaat
      541 ctgttacttt gtgtacatca tttgctatat attgtcaata tgctattgtt gtaaagcaaa
      601 tgatatacgg aggctttatg aaatcaggtg ttcagctcaa tctcagagca agagaatcac
      661 agcggatact cattgatgct gctgcagaaa ttcttcataa gtcgcgtaca gatttcattc
      721 tggaaatggc ctgtaaagct gctgaagatg ttattctcga ccggcgggtg tttaatttta
      781 atgacaggca atatgaagag ttcattgaaa tgcttgatgc tccggttgca gatgatcctg
      841 ccatcgaaaa attgctggca aggaaacctc aatgggatgt gtaactgcac cagaaccttt
      901 atcctcattt catcaggtcg ctgaatttgt cagcggtgaa gctgtacttg atgactggtt
      961 aaagcagaag gggctaaaaa atcaggcaat cggagccacc cggacttttg ttgtatgcag
     1021 aaaaggtacg caacaaatag tcggttttta ctcactggcc actggtagtg tcaatcatac
     1081 agaagctaca ggtaaccttc ggcgcaatat gccagacccc attcctgtaa ttatacttgc
     1141 acgcctggct gtagatgtgt catttcgagg aaaagggctt ggcgcggatt tacttcatga
     1201 tgcagttcgt cgttgttaca gggttgccga aaatatcggt gtgcgtgcaa ttatggttca
     1261 tgcgcttacg gaaaacgcaa aacagtttta tattcatcat ggttttaagc catcaaaaac
     1321 tcaggtgcaa acattgtttc ttaaactccc tcaataggga gtaatgcact ggtctgggca
     1381 tcatatctgg atgtgcgtcg caacaaggga gccccccgga tgcgacgggc agactttgaa
     1441 aatgttcgac cagcaacgtg atggcaatct atacaagatc tggaaccggc tatgttcggg
     1501 aacatggttt ccgccaccag tgctggaaaa acggatcccg aagtcgaatg gcaaggaacg
     1561 cattctgggg atcccaacag tatccgatcg aattgctcag ggagcgataa aacttttcat
     1621 ggaagaaaag cttgacccga ttttccatgc tgattcatat ggttaccgac caggcaaatc
     1681 agctcatgac gctctgaaac aatgtgccat ccggtgctgg cgttatagct ggatactgga
     1741 agtggacatc agtgcgtttt tcgaccatgt gaaacatgat ctggtgctca aagctctgga
     1801 acaccacggg atgcctaaat gggtcatcct gtactgtcga cgctggatgg aagcgccaat
     1861 gcagagttgt gaaaatggag aattaataac ccgaacacga ggcactccgc agggcggggt
     1921 cattagtccg ctacttgcta acttgttcct tcactatgca tttgatttgt ggatggaaag
     1981 agaatatcgg ggggtaccgt ttgagaggta cgctgacgat attgtagtac attgttcacg
     2041 aatgagtgat gcaacaaggc taaagaacag gttatcggag cgcttctcgg aagtcgggct
     2101 ggtcctgaat gcagggaaaa cgaatatcgc ctacattgac acgtttaaaa ggagaaacgt
     2161 cgcaacgagt ttcacctttc tcggatatga cttcaaagtg cgtacgctga agaatttcaa
     2221 aggcgaacgg taccgaaaat gcatgccggg tgcgtcaaat gcagcaatgc gcaaaataac
     2281 agaaacaatc aagaagtggc gtatacatcg ctcaacagct gagagtttgc tggattttgc
     2341 gaggcgctac aatacgatag tgagaggctg gatcgagtac tacggaaagt tctggtccag
     2401 aaatttcaac tatcgactgt ggagtgcaat gcagtcacgt ctgctcaagt ggatgcagtc
     2461 taaatacaga ctttcgaacc ggaaggctca gcgaaagctg acgctggtaa ggaaggagta
     2521 tccgaagcta tttgttcact ggtatttact acgtgcatca aatgagtgat caagagccgt
     2581 atgacgcgag agtgtcacgt acggttccgt gagcagccgg ggagtgaaat tctctccggc
     2641 tgactcgact cgcgggttcc gacagcgatg acgaacatgc ggcggtgttg tactcgctga
     2701 tcggcacatg ccgtctgaac aatgtggaac cagaaaatgg ctgcgttacg tcattgagca
     2761 tatccaggac tggccggcaa accgggtacg tgatctgttg ccctagaaag ttgatctgag
     2821 ctctcagtaa atatcaatac ggttctgacg agccgcttac ttttggcgta attctgctgc
     2881 gcctttactg aacacaatgc cgtattcagc aagaagtcca cgaatgtgat ttatcagttg
     2941 tgtcctttcc attgaaacca accgacgcat attcaaaaaa gtttggacgt cctgttgctc
     3001 cagagttttt attggtacgg gtcgtatagt tccatgttgg caggcctcag ctattgcctg
     3061 tgcgtcgtta taatcatttt tctggcaccg ctgataagct ttaacatgtt gtgccggaag
     3121 caatatt
//
LOCUS       DAJTNN010000275         3104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTNN010000275 DAJTNN010000000
VERSION     DAJTNN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.281"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            165..464
                     /locus_tag="OO150_004058"
     CDS             165..464
                     /locus_tag="OO150_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5518002.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            559..1470
                     /gene="glyQ"
                     /locus_tag="OO150_004059"
     CDS             559..1470
                     /gene="glyQ"
                     /locus_tag="OO150_004059"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5518003.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            1480..>3104
                     /gene="glyS"
                     /locus_tag="OO150_004060"
     CDS             1480..>3104
                     /gene="glyS"
                     /locus_tag="OO150_004060"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5518004.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLVESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYT"
BASE COUNT          745 a          815 c          850 g          694 t
ORIGIN      
        1 gtatctggtt aaaaaaagtc tgaccgataa atcatttggt tggcgcactg gaataatctg
       61 agttttatca ctacagctta tagaggctta aggaaattcg taagatatca gccactatac
      121 cgatataaat aataagactc acctgcaaac cagacggtaa tttaatgatg atgaacgctt
      181 tctttccggc aatggcgctt atggtgctag tgggttgttc tacaccgtca cccgtgcaga
      241 aagcacaacg ggtaaaggtt gatcctctgc gttcgttgaa tatggaagcg ttatgcaagg
      301 atcaggcggc aaaacgttat aacaccggcg agcaaaaaat cgacgtcacc gccttcgaac
      361 agttccaggg aagctatgaa atgcgcggtt ataccttccg taaagagcag tttgtctgtt
      421 cttttgacgc agatggccat tttttgcatc tttccatgcg ttaagccctg ctttttcccg
      481 tttcgtactg tatatcttcc atccagcggg tatactgatc ccttccttta aatccacacg
      541 tatccagcac gaaataatat gcaaaagttt gataccagga ccttccaggg cttgatcctg
      601 accttacagg attactgggc tcgccagggc tgcaccattg ttcaaccatt ggacatggaa
      661 gtcggcgcgg gaacctctca cccaatgacc tgtctgcgcg cgctggggcc agaaccgatg
      721 gcggctgctt atgttcagcc ttctcgtcgc ccgaccgatg gtcgctacgg cgaaaacccc
      781 aaccgtttac agcactacta tcagttccag gtggtcatta agccatcgcc ggacaatatt
      841 caggagctgt acctcggttc tctgaaagag ctgggcatgg acccgactat tcacgacatc
      901 cgtttcgtgg aagataactg ggaaaacccg actctgggtg cctggggact gggctgggaa
      961 gtgtggctaa acggcatgga agtgacgcag ttcacttact tccagcaggt tggtggtctg
     1021 gagtgtaaac cggttaccgg cgagatcacc tacggtctgg aacgtctggc catgtacatt
     1081 cagggcgtag acagcgttta cgacctggtc tggagcgacg gcccgctggg taaaaccacc
     1141 tacggcgacg tgttccatca gaacgaagtg gagcagtcca cttacaactt cgaatacgcg
     1201 gatgtggact tcctgttcac ctgctttgag cagtacgaga aagaagcgca gcagctgctg
     1261 gcgctggaaa atccgctgcc gctgccagcc tacgagcgta ttctgaaagc cgcccacagc
     1321 ttcaacctgc tggatgcgcg taaagccatc tccgtcaccg agcgtcagcg ctacattctg
     1381 cgcattcgca ccctgaccaa agcagtggca gaagcatact acgcttcccg tgaagccctc
     1441 ggcttcccga tgtgcaacaa agataagtaa gaggcggcta tgtctgagaa aacttttctg
     1501 gtggaaatcg gcactgaaga gctgccacca aaagcactgc gcagcctggt tgagtccttt
     1561 gctgcgaatt ttactgtgga gctggataac gctggcctcg cacacggcac cgttcaatgg
     1621 tttgctgctc cgcgtcgtct ggcgctgaaa gtagctaacc tggcggaagc gcaaccggat
     1681 cgtgaaatcg aaaaacgcgg cccggcgatt gcccaggcgt tcgacgctga aggcaaaccg
     1741 agcaaagcgg cagaaggttg ggcgcgtggt tgcggtatta ccgttgacca ggctgagcgt
     1801 ctgactaccg ataaaggcga atggctgctg tatcgcgccc atgtgaaggg cgaaagcacc
     1861 gaagcactgc tgccgaatat ggttgcgact tctctggcga aactgccgat cccgaaactg
     1921 atgcgttggg gcgcaagcga cgtgcacttc gtgcgtccgg tgcacaccgt gaccctgctg
     1981 ctgggcgaca aagtcattcc ggcaactatt ctgggcattc agtccgatcg cgtgattcgc
     2041 ggccaccgct ttatgggcga accggaattc accatcgaca acgccgatca gtatccggaa
     2101 attctgcgtg agcgcgggaa agtcatcgcc gattacgaag aacgtaaagc gaagattaaa
     2161 gccgatgccg aagaagcagc gcgtaagatt ggcggtaacg ctgacttaag cgaaagcctg
     2221 ctggaagaag tggcttcgct ggtggagtgg ccggtcgttc tgaccgcaaa attcgaagag
     2281 aaattcctcg cggtgccggc tgaagccctg gtttacacca tgaaaggtga ccagaaatac
     2341 ttcccggtgt atgcgaacga tggcaaactg ctgccgaact tcatcttcgt tgccaacatc
     2401 gaatcgaaag atccgcagca aattatctct ggtaacgaga aagtcgttcg tccacgtctg
     2461 gcggatgccg agttcttctt caataccgac cgtaaaaaac gtctggaaga taacctgccg
     2521 cgcctgcaaa ccgtgctgtt ccagcaacag ctgggtacgc tgcgtgacaa aactgaccgc
     2581 atccaggcgc tggctggctg gattgctgaa cagattggcg ctgacgttaa ccacgcaacc
     2641 cgtgcgggcc tgctgtctaa gtgtgacctg atgaccaaca tggtcttcga gttcaccgac
     2701 acccaaggcg ttatggggat gcactacgcg cgtcacgatg gcgaagcgga agacgtagcc
     2761 gtcgcgctga atgagcagta tcagccgcgc tttgccggtg atgacctgcc gtccaacccg
     2821 gtagcttgtg cgctggcgat tgctgataag atggatactc tggcgggtat cttcggtatc
     2881 ggtcagcatc cgaaaggcga caaagacccg tttgcgctgc gtcgtgccgc acttggcgtg
     2941 ctgcgaatta tcgttgagaa gaacctcaac cttgatctgc aaacgctgac cgaagaagca
     3001 gtgcgtctgt atggcgataa gctgactaac gccaacgtgg ttgatgatgt tatcgacttt
     3061 atgctcggtc gcttccgcgc ctggtatcag gacgaaggtt acac
//
LOCUS       DAJTNN010000276         3061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTNN010000276 DAJTNN010000000
VERSION     DAJTNN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.282"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..744
                     /locus_tag="OO150_004061"
     CDS             <1..744
                     /locus_tag="OO150_004061"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5518005.1"
                     /translation="FYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVA
                     INDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSA
                     WKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGG
                     DGVYYSDISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSS
                     MALSCEYTNNPDLSIANVISLVENAERLN"
     gene            711..1670
                     /gene="psuG"
                     /locus_tag="OO150_004062"
     CDS             711..1670
                     /gene="psuG"
                     /locus_tag="OO150_004062"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5518006.1"
                     /translation="MVSGERRTSELKISPELLQISPEVQDALKNKKPVVALESTIISH
                     GMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVS
                     RRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQEL
                     ANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDS
                     ASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKES
                     TLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1764..3014
                     /locus_tag="OO150_004063"
     CDS             1764..3014
                     /locus_tag="OO150_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5518007.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
BASE COUNT          723 a          692 c          828 g          818 t
ORIGIN      
        1 ttttatggtc aatcgctgct aacgcaaacc aatcaatctg gcgtttatgt cgataaatgc
       61 ctgattgtgc cgggagaaaa tacgtctagt tatttatcat tactcgataa taccggtgaa
      121 atgctggttg ctataaatga catgaatatt agcaacgcta ttacagctga atatctcgca
      181 cagcaccgtg aatttattca gagggcaaag gtcattgtcg cggactgtaa tatcagtgaa
      241 gaggcactgg catggattct ggataatgcc gccaacgtac ccgtatttgt cgatccggtt
      301 tccgcatgga aatgtgtcaa agtgcgcgac cgtctaaatc agatccacac tctgaagcca
      361 aaccgccttg aagcggaaac cctgagtggg attgcgctgt cagggcgtga agatgtggca
      421 aaagttgctg cctggttcca tcaacatggc ctgaaccgac tggtattgag catgggcggc
      481 gacggcgttt attacagcga tatcagcggt gaaaatagct ggtctgcgcc gatcaaaacc
      541 aatgttatta atgttaccgg agcgggcgat gccatgatgg cgggacttgc ttcgtgttgg
      601 gtagacggaa tgccgtttgc cgaatctgtt cgtttcgcac agggatgttc gtcaatggcg
      661 ctctcctgtg aatacaccaa taaccccgat ttatcgattg ccaacgttat atcgttagtg
      721 gagaacgcag aacgtctgaa ttaaaaattt cccctgaatt attacaaatt tccccggaag
      781 tgcaggacgc tttaaaaaac aaaaaaccgg ttgtggcgct ggaatcgacc attatttctc
      841 acgggatgcc gttcccacaa aatgcccaga ccgcaattga agtagaagaa actattcgta
      901 aacagggcgc tgtacctgcc actatcgcca ttattggcgg cgtgatgaaa gtgggtttaa
      961 gcaaagaaga aattgaatta ctgggtcgtg aagggcataa cgtgaccaaa gttagtcgtc
     1021 gcgatttacc ttttgttgtt gccgccggaa aaaatggcgc aaccactgtg gcttcaacga
     1081 tgattattgc ggcgcttgcc ggaattaaag tatttgccac cgggggaatt ggtggtgtgc
     1141 atcgcggggc ggaacatacc ttcgatattt ctgccgattt gcaagaactg gcaaatacta
     1201 atgtcaccgt tgtttgtgcc ggggcgaaat ctattctcga tttaggatta accactgagt
     1261 atttagaaac cttcggtgtg ccgttaattg gctatcagac taaagcgctg cctgcgtttt
     1321 tctgccgtac cagctcgttt gacgtcagta ttcgtctcga cagcgccagt gaaattgccc
     1381 gtgcaatggc ggtgaaatgg caaagcgggc tgaacggtgg cctcgtggta gcgaacccga
     1441 tcccggaaca gtttgcgatg ccggaggaat ctatcaatgc agccatagat caagccgtcg
     1501 ccgaagccga agagcagggc gttattggta aagaaagtac actgttcctg ctggcgcgcg
     1561 ttgctgagct gaccggcggt gacagcctga aatccaatat ccagctggta ttcaacaacg
     1621 ccattctggc gagtgaaatt gccaaagaat accagcgtct cgcgggttaa tttccgacgg
     1681 gcagaaagcc gtcgcccgta ctcatcaagc ataccaaaac ctggcgctga cgccgggttt
     1741 actggcatga agggaaaatc attatggata taatgagaag tgttgtgggg atggtggtgt
     1801 tactggcaat agcatttctg ttgtcagtga ataaaaagag catcagtttg cgcacggttg
     1861 gagccgcact gctgctgcaa atcgctattg gtggcatcat gctctacttc ccaccgggaa
     1921 agtgggcagt agaacaggcg gcattaggcg ttcataaagt gatgtcttac agtgatgccg
     1981 gtagcgcctt catttttggt tcgctggttg ggccgaaaat ggatgtcctg tttgacggtg
     2041 cgggttttat cttcgccttt cgcgtacttc cggcgattat tttcgttact gcgctcatca
     2101 gtctgctgta ctacattggc gtgatggggc tgctgattcg catccttggc agcattttcc
     2161 agaaagctct taacatcagc aaaatcgaat cttttgtcgc agtcacaact attttcctcg
     2221 ggcaaaatga gatcccggcg atcgttaaac cgtttatcga tcgcatgaat cgcaacgagt
     2281 tgtttaccgc aatttgtagc gggatggcgt ccattgctgg ttcgatgatg attggttatg
     2341 ccggaatggg cgtaccaatt gactacctgt tagcggcatc gctgatggcg atcccaggtg
     2401 gtattttgtt tgcacgtatt cttagcccgg ccaccgagcc ttcgcaggtc acatttgaaa
     2461 atctgtcgtt cagcgaaacg ccgccaaaaa gctttatcga agcggcggcg agcggtgcga
     2521 tgaccgggct gaaaatcgcc gctggtgtag cgacggtggt aatggcattt gtcgcaatta
     2581 ttgcgctgat caacggcatt atcggcggaa ttggtggctg gtttggtttc gccaatgcct
     2641 ctctggaaag tatttttggc tatgtgctgg caccgctggc gtggatcatg ggtgtggact
     2701 ggagtgatgc caatcttgcg ggtagcctga ttgggcagaa actggcgatt aacgaattcg
     2761 tcgcttacct gagtttctcc ccatacctgc aaacgggcgg cacgctggaa gtgaaaacca
     2821 ttgcgattat ctcctttgcg ctttgtggtt ttgctaactt tggttctatc ggtgttgtcg
     2881 ttggcgcatt ttcggctatt tcgccaaaac gcgcgccgga aatcgcccag cttggtttac
     2941 gggcgctggc agcagcaacg ctttccaacc tgatgagtgc gactattgcc gggttcttta
     3001 ttggtttagc ttgagtttca ttgccggatg tacgcgctta ttaagcgttg catccggcaa
     3061 t
//
LOCUS       DAJTNN010000277         2942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTNN010000277 DAJTNN010000000
VERSION     DAJTNN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.283"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            577..1293
                     /locus_tag="OO150_004064"
     CDS             577..1293
                     /locus_tag="OO150_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518008.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1845..2249
                     /locus_tag="OO150_004065"
     CDS             1845..2249
                     /locus_tag="OO150_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518009.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2312..2942
                     /locus_tag="OO150_004066"
                     /pseudo
     CDS             2312..2942
                     /locus_tag="OO150_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          824 a          605 c          667 g          846 t
ORIGIN      
        1 ttgtgggcca taaatattcc aaaaaaatag cccgctccgt ttattaccgg aacgggcgag
       61 agcatgacat tgtctgtaat tataatgaga ctgctgattc tacttatttt taatttcaag
      121 aaaagcgttt tctacaaaga attatgagat ttaatgtttt gtttagacaa tattgtcatt
      181 ctgttcaata atgaaagtct tcatcaacaa caagtaaaaa caacgtccct ctcatcgaac
      241 cggacttgca gatttcccgc atccggctct cttgcaacac atcagacctt cacgttcggg
      301 cggttatggg gaaggtccag caagctgtat gagccctaac tgtttgaata ccagtagatt
      361 accagaaact tctatcgatt gaagacatta ccaactagcg ttactaaatg ttgaccaagt
      421 tttgttcact tgtctggttt tgatagtagc attctgcaga gtattttgat atgataaata
      481 tgcattaaca atgatattat tgttaatttc ctagcatgaa aattaaaaga tcaacttttt
      541 atctcgaata ttttttacat tatcagttgg tttcttatga atgacaattc attgcttaga
      601 aattcttcat tattcatcgc ctatatggga tgtgtagggt gggtcagtgc ttattcttat
      661 ggctggggaa cctcttttta ctatggattt ccatggtggg ttgttggtgc aggtttggat
      721 gatgttgcca gaagtttact ctatgctatc atagtaatgg gtatactttt taccggttgg
      781 gggattggta ttttattttt tcttcttata aagaaaagaa gtaaaataca agacttgagt
      841 ttttttagac tattttttgc tattacccta ctgtttttcc ctgtaatatt cgaactttta
      901 attctcaaac aatatttcat acttccatta tcactttcat gcattattag ttcattggtt
      961 atttcaataa taattcgcat atatggacgg atcttttctg tatcatgttt ttcagatatc
     1021 ccttttgtta gagaacaccg cattaagtta attatggctg gttttttggt ttatttttgg
     1081 cttttttcct ttcttgttgg ctggtataaa cctcaattaa aaaaagaata tcagatgctt
     1141 tgttataata atagttggta ttatgttctt gctcgttatg attcacggct cgttttgtct
     1201 agttcattta aagatgacag taacagattc ttaatattta atacagaaca gtcgggattt
     1261 tatgaaatca atgatgttta tgtcagaaag taataatcct ctcccacaaa gtgaattaaa
     1321 cgaatatata atgcgttcga agtatatgta ttgagctgtc atactgagaa acaaacctta
     1381 gggcagcaca attaaagcca gaaacaacta tgcaagaaag ttactaaata agtgctcgga
     1441 acagtaaaac actcaggggg gactcagtcc agattatgta atcagataaa ttgccaaaca
     1501 tcacaaatca ccagcaggac tgagtgatgc cgagcattgc accaataccc cgtgatgaac
     1561 gatgcctgat gcagaaagct ccctataaaa caaagaacaa aaactacgac cggcgattca
     1621 ccgtcatact gatgctgcat cgtggtgaca ctgtctgtca ctttcatgtt gactgaacaa
     1681 ctctgctgcg aagaaggtgt accgccggcg ggatttgatt gtccggcgtc tagcttccac
     1741 ttccctgatg gaactgaaag ttgttatggc cggtcagctt gccctgtccg caggacatga
     1801 ggtgctgtga cggacagcac cagtaacgga aagtgacaat aaaaatgaat actgcgctga
     1861 tagttgcact aatgtgtatg tggtatgcgg tacccgctgc tgcaaaagag actttactgg
     1921 ctatgccacg taattcaaca gaacattgct atgcggagat taatgtgcac ggaccatatg
     1981 gggtgtattt ccgagtggtc ccgcatcccc ccgggggaaa gagctgggtc gaatgtaaca
     2041 gtgactacta ttactctgat aaaccaccgg gagtacagat actcggaacc cgtgcagggt
     2101 gtcgggtata cggaatctgc gggaccacct cgacactgca tgtggctgga agaggggttg
     2161 tgtgtattaa aaatatatgc agtccacgtg ggatgataat tcacaggata cggaaacgtc
     2221 ctgtagtggc tgtaagcgat gagatgtgat cttatccagc aatagtggac acgcggctaa
     2281 gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa ccagcaaaaa
     2341 aacacgtaaa cagcattcgc ctgaattttg cagtgaagcc ctgaagcttg ctgaacgcat
     2401 cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct atgcgtggcg
     2461 cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg ccgctgaaaa
     2521 tgcccgcctg aagcggcaac tggcggtgca ggcagaggaa ctggctatcc tccaaaaggc
     2581 cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct
     2641 gagttcagca tcaaggcaat gtgccgcgcg ctccgggtgg cccgcagcgg ctggtatacg
     2701 tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca ctgcgacagc
     2761 gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc acgcctgacg
     2821 gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc
     2881 cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat
     2941 gg
//
LOCUS       DAJTNN010000278         2908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTNN010000278 DAJTNN010000000
VERSION     DAJTNN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.284"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1312
                     /locus_tag="OO150_004067"
     CDS             <1..1312
                     /locus_tag="OO150_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5518010.1"
                     /translation="VFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPG
                     LAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKSDGSLIKEYSVVALQGLTLVLFIFF
                     MWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHI
                     DFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMG
                     MMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPL
                     KVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVQVAILIMLPTLGIGDMNNLYKWLL
                     NLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLT
                     GIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(1313..2221)
                     /locus_tag="OO150_004068"
     CDS             complement(1313..2221)
                     /locus_tag="OO150_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5518011.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINDARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQISQSQRHRPQ"
     gene            2321..>2908
                     /locus_tag="OO150_004069"
     CDS             2321..>2908
                     /locus_tag="OO150_004069"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCR5518012.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT          739 a          620 c          721 g          828 t
ORIGIN      
        1 tgttttttca tgggtgttta tctttgcgct ctatttcaca ccttatgcgc taattgttgg
       61 tcagttaggc tcgaccttca aagatgggaa gggcggggtc agtacctgga ttaaacacac
      121 gatgggaccc ggactggctt atctcgccgc gtggacctac tgggtggtgc atattcccta
      181 tctggcacaa aaaccccagg caattctgat tgcgctcggt tgggcgatga aaagcgacgg
      241 ttcgctaatc aaagaatatt cagtcgtagc gttacagggg ttaacgctgg tgctgtttat
      301 cttctttatg tgggttgctt cacgcggtat gaaatcgctg aaaatcgtcg gttctgtggc
      361 agggattgcg atgtttgtta tgtcgctcct gtatgtggcg atggcggtaa ccgcgcctgc
      421 gattactgaa gtgcatattg cgaccacaaa cattacctgg gaaacgttca ttcctcatat
      481 cgactttacc tacattacca ctatttcaat gctggttttc gcggttggcg gagcagagaa
      541 gatttctcct tacgttaatc aaacgcgcaa cccaggaaaa gaatttccaa aagggatgtt
      601 atgcctggcg gtgatggttg cggtttgtgc cattctgggc tcactggcga tggggatgat
      661 gtttgactcg cgtaatatcc cggatgactt aatgactaac ggtcaatatt acgcctttca
      721 gaagctgggc gagtattaca acatgggtaa tactttaatg gtgatttacg ccattgcgaa
      781 taccctggga caagtagctg cgttggtatt ctcgattgat gccccgctta aagtgctatt
      841 aggtgatgct gacagcaaat atattccagc cagtttatgt cgtaccaacg cttctggtac
      901 gcccgttaat ggctattttc tgaccctggt gcaggtggca attctgatta tgctgccgac
      961 tctcggcatc ggtgatatga acaatctcta taaatggctg ttgaatctta actcggtagt
     1021 gatgccgctg cgttatttat gggtatttgt tgcatttatt gcagtcgttc gcctggcgca
     1081 gaaatataaa ccagaatatg tctttattcg taataagcct ctggcaatga ccgtcgggat
     1141 ttggtgtttt gcctttaccg cctttgcctg tttgacgggg atcttcccga aaatggaagc
     1201 cttcactgca gagtggacct tccagttggc gctgaatgtt gcaacgccgt ttgtgctggt
     1261 aggattagga ctgattttcc cgctgctggc gcgtaaagcg aatagtaaat aattattgtg
     1321 gtcggtgacg ctgactctgg ctgatctgac ccgtttttac ataatcgaga aatgcgcgca
     1381 agccgcagga catatactgg cggtttggat aatagatctg gaagcctgga cgctctgtgc
     1441 tccagtctcc cagtacacat tctaaacgac cagtatcaag gtactccttg atctgttcat
     1501 agagcagata cccgatacca gcacccatca aaacggcctc cagttcagca tcaacatcat
     1561 ccagaatgat atttcctgcg acagcaattt ccagctcctt tgcaaattgc cagtggaaag
     1621 gtttgccgct gggataacga aaaacaacac attgatgatt aagcagatca tgaggatggc
     1681 ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat ttcactggcg
     1741 ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga acgccagcgt
     1801 caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg actttaatat
     1861 caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt gcagctaccc
     1921 gggcgtcatt gattttgagc gttcctgtcg gtgtcagacg aaaatcattc atctcatcta
     1981 acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta gaacccgctt
     2041 ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga cgttgttcca
     2101 gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg cccgctgcac
     2161 gaaagctttg attacgggcc acggcaaaga aagtggcaaa gtcagacata ttcatccgca
     2221 ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacaggat tatgccgcct
     2281 gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg aacgtattta
     2341 tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg atgcgatcca
     2401 tcagcgggca atggagcgga aaatacaggt gacggaactc gatttatatc ggcgtaattt
     2461 caacccagtg atgacgccgg aagatgaacc agactggaag aatatggata aacgttattc
     2521 tccagaggtt catcagcttt attcagagct gcttgaacat gacacgttag tggtggtttt
     2581 tcctctctgg tggtacagct tcccggcaat gctaaaagga tatattgaca gagtatggaa
     2641 taatgggctg gcttatggag atgggcacaa attaccattc aataaagttc gttgggtggc
     2701 gctggttgga ggagacaaag aatcatttgt ccagatgggc tgggaaaaaa atataagcga
     2761 ttatttgaaa aatatgtgca gttatcttgg tattgaagat gccgatgtca ctttcttgtg
     2821 taatacagtg gtattcgatg gggaagaact tcatgcgagc tattatcagt cgttattatc
     2881 tcaggtacgg gatatggtag atgcacta
//
LOCUS       DAJTNN010000279         2905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTNN010000279 DAJTNN010000000
VERSION     DAJTNN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.285"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..434
                     /locus_tag="OO150_004070"
     CDS             <1..434
                     /locus_tag="OO150_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5518013.1"
                     /translation="GMAEDLQSLVGGTVVRRKVYARFLDAVNFVNGNSDADPEQEVIS
                     RWRIEQCSELSAVSASFVLATPTETDGAVFPGRIMLANTCMWTYRSDECGYTGRAVAD
                     EFDKPTTDIRKDKCSKCMRGCELRNNTGNFGGFLSINKLSQ"
     gene            439..1182
                     /locus_tag="OO150_004071"
     CDS             439..1182
                     /locus_tag="OO150_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5518014.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            1119..1721
                     /locus_tag="OO150_004072"
     CDS             1119..1721
                     /locus_tag="OO150_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5518015.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1782..>2905
                     /locus_tag="OO150_004073"
     CDS             1782..>2905
                     /locus_tag="OO150_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5518016.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFSVQALVENTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMVWCR
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRS"
BASE COUNT          622 a          720 c          949 g          614 t
ORIGIN      
        1 ccgggatggc ggaagatctg cagagtctgg tcggcggaac ggtggtccgg cgtaaggttt
       61 acgcccgttt tctggatgcg gtgaacttcg tcaacggaaa cagtgacgcc gatccggagc
      121 aggaggtgat cagccgctgg cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct
      181 cttttgtgct ggccacacca acggagacgg atggcgcggt tttcccgggg cgtatcatgc
      241 tggcgaatac ctgtatgtgg acctaccgtt ctgatgagtg tggttacacg ggcagggcag
      301 tggctgacga gttcgacaaa ccaacgacgg atatccggaa ggacaaatgc agcaagtgta
      361 tgcgcgggtg tgagttgcgc aacaataccg gtaatttcgg cggtttcctt tccatcaata
      421 aactttctca gtaaatccat gacacagaca gaatcagcga ttctggcgca cgcccggcga
      481 tgtgcgccag cggagtcgtg tggcttcgtg gtgagaacgc cggaggggga gcggtatatc
      541 ccttgtgtga atatttctgc agagccggag gcgtattttc gtatcgcacc ggaagactgg
      601 ctgcgggcag agatgcaggg ggagattgtg gcactggtcc acagtcatcc cggtggtctg
      661 ccctggctga gtgaggctga ccggcggctg caggtgcaga gtgatttgcc gtggtggctg
      721 gtctgccggg gggcgattca caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt
      781 gagcacgggg tgacggactg ttacacgctg ttccgggatg cttatcatct ggcggggatt
      841 gagatgccgg attttcatcg cggggatgac tggtggcgtc acggtcagaa tctctatctg
      901 gataatctgg aggccacagg gctgtatcag gtgccgttgt catcagcaca accgggcgat
      961 gtgctgctgt gctgttttgg ttcatcggtg ccgaatcatg ccgccattta ctgcggcgac
     1021 ggtgagctgc tgcaccatat tcctgaacaa ctgagtaaac gagagaggta taccgacaaa
     1081 tggcagcgac gcacacactc cctctggcgt caccgggaat ggcacgcatc tgcctttacg
     1141 gggatttgca acgatttggc cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca
     1201 tccgcgcact ggccacacag ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc
     1261 aggtgcgcat tgccgggcgt gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc
     1321 cgctggcaaa tggtgcagtg atccacatag taccgcgtct ggtgggagct aaaagtggcg
     1381 gtgtgtttca ggcggtgctg ggggcagctg ttatggcggt tgctatatgg atgccggggg
     1441 taggaattat ggcgagtaat ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg
     1501 ttgcacagat gctggcaccg aaagccagaa ctccccgtac acagacaacg gataacggca
     1561 aacagaacac ctgtttctcc tcactggata acatggttgc ccagggcaat gttctgcctg
     1621 ttctgtacgg tgaaatgcgc gtggggtcgc gggtggtatc tcaggagatc agcacggcag
     1681 acgaagggga tggtggtcag gttgtggtga ttggtcgctg atgcaaaatg tttcatgtga
     1741 aaccgcctgc gggcggtttt gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta
     1801 aggggcatac cccgcgcgaa gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga
     1861 ttgatgccat cagcgaaggg ccggttgacg gtccggtgga tggattaaaa agcgtgctgc
     1921 tgaacggtac gccagtcctg gacagcgagg ggaagacaaa cttttccggt gttacggtgg
     1981 tgttccgcgc cggcgagcag gagcagacac cgccggaggg gtttgaatct tccggctcag
     2041 agactgtgct gggtacggaa gtgaaatacg acacgccgat cacccggacc atcacgtcgg
     2101 caaacattga tcgtctgcgc ctgaccttca gtgtgcaggc actggtggaa aacacctcaa
     2161 agggggaccg gaatccgtcg gaagtccgcc tgctggttca gatacagcgt aacggtggct
     2221 gggtgacgga aaaagacatc accattaagg gcaaaaccac ttcgcagtat ctggcctcgg
     2281 tggtggtggg taacctgccg ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg
     2341 acagcaccac agaccagctg cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg
     2401 atgtgaaaca gtgctacccg aacacggcgc tggtcggcgt gcaggtggat tcagagcagt
     2461 tcggtaacca gcaggtgagt cgcaattatc atcttcgcgg gcgcattctg caggtgccgt
     2521 cgaactataa cccgcagacg cggcaataca gcggtatctg ggacggaacg cttaagccag
     2581 catacagcaa caacatggtc tggtgtcggt gggacatgct cactcatccg cgctacggca
     2641 tggggaaacg tcttggtgcg gcagatgtgg acaaatgggc gctgtatgtc atcggccagt
     2701 actgcgacca gtcagtgccg gacggatttg gcggcacgga gccgcgcatc acctgtaacg
     2761 cttacctgac cacacagcgt aaggcgtggg atgttctcag tgatttctgc tcggcgatgc
     2821 gctgtatgcc ggtatggaac gggcagacgc tgacgttcgt gcaggaccga ccgtcggata
     2881 aagtgtggac ctataaccgc agtaa
//
LOCUS       DAJTNN010000280         2851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTNN010000280 DAJTNN010000000
VERSION     DAJTNN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.286"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..203
                     /locus_tag="OO150_004074"
     CDS             <1..203
                     /locus_tag="OO150_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518017.1"
                     /translation="VNNNPGYVVEIFEEKMGNELKIRTRMMEILRDGKINICEYINKE
                     QLEKLNPPEDLRIAIKKLGWNN"
     gene            complement(275..1279)
                     /gene="argF"
                     /locus_tag="OO150_004075"
     CDS             complement(275..1279)
                     /gene="argF"
                     /locus_tag="OO150_004075"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5518018.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1441..1857
                     /gene="rraB"
                     /locus_tag="OO150_004076"
     CDS             1441..1857
                     /gene="rraB"
                     /locus_tag="OO150_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5518019.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1903..2405)
                     /locus_tag="OO150_004077"
                     /pseudo
     CDS             complement(1903..2405)
                     /locus_tag="OO150_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2598..2795
                     /locus_tag="OO150_004078"
                     /pseudo
     CDS             2598..2795
                     /locus_tag="OO150_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
BASE COUNT          773 a          636 c          656 g          786 t
ORIGIN      
        1 ttgtaaataa caatccgggt tatgtcgttg agatttttga agaaaagatg ggtaatgaat
       61 taaaaattag aacaagaatg atggaaatcc tccgggatgg gaaaataaat atatgtgaat
      121 atattaataa agaacagtta gaaaaattaa atcctccgga agatttacgt atagcaataa
      181 aaaaattggg gtggaataat tgaactatta ctgcataaaa taatgtgatg ccgggatgtt
      241 tttgtatttc ccggcatctt tattgcgata tcaattattt actgagcgtc gcgaccatca
      301 ccgctttgat ggtatgcatg cggttttccg cctgatcaaa aacaatgctg gcggcagatt
      361 cgaagacctc atcagtcact tccattccgc catgtaggcc aaactcttcc gccattttct
      421 taccaagcgt cgtctggtcg tcatgaaacg cgggcaggca gtggaggaat ttgacctccg
      481 ggttaccggt caactgcatc atcttgctgt tcacctgata atcacgcagt aatgcaatcc
      541 gttctgccca tttctctttt gcttccccca tcgacaccca tacatcggta tagataaagt
      601 cagcaccttc aactcctttg gctacatctt ccgtcagcgt aatattcccg ccattttgct
      661 gtgccaaggc gcggcattcc gtaaccagcg cagcttccgg ccagcatgcc tgtggcgcga
      721 ccagacgcaa atccagaccg gtaagcgccg cagcttcgag catcgaattg cccatgttgt
      781 tacgtgcgtc acctgcatag accagcgtca tttcgttgaa tgctttaccg ggtaaatgct
      841 cctgcatggt gaggagatcc gccagcaact gcgtggggtg aaactcattg gtcaggccgt
      901 tccataccgg cacgccagcg tattccgcca gtgtttcggc aatctcctga ccatagccgc
      961 gatactgaat accgtcatac atgcgaccca gcacgcgggc agtgtcttta atcgactctt
     1021 tatgaccaat ctgactgccg cttgggccga gataagtaac gcgagcaccc tggtcatatg
     1081 cggcaacttc gaaagagcat cgggtacggg tcgagtcttt ttcgaagatg agcgcgatgt
     1141 ttttaccagt gagtttggct tcttctttgc cgcttttctt atcggctttc agcttcgcgg
     1201 ctaactgcag caggctgttg agttcagctg gcgtgaaatc gagtaatttc aggaaatgct
     1261 tatgataaaa cccggacata gatccctcct gtggctaacg cctcaatgaa ttaaaattca
     1321 atttatatgg atgattattc atttgcaagt ctaaagcata aatctttgtc acaaaggtgg
     1381 aggcaatgtc agtggtatgt gacaataaga gtatcggcag gacattaaga ggaatgagcc
     1441 atggcaaacc cggaacaact ggaagaacag cgtgaagaaa ctcgtttgat tattgaagaa
     1501 ttactggaag atggcagcga tccggacgcg ctgtacacca tcgaacacca tctttccgca
     1561 gacgatctgg aaaccctgga aaaagcagca gttgaagcgt ttaaactcgg ttacgaagtg
     1621 accgatccag aagagctgga agttgaagat ggtgatatcg tgatttgctg cgacatcctc
     1681 agcgagtgcg cgttgaatgc cgatctgatc gatgcccagg ttgaacaact gatgacattg
     1741 gcggagaaat ttgacgttga gtacgacggt tggggcactt attttgaaga tcctaatggc
     1801 gaagacggcg acgatgaaga ttttgtcgat gaagacgatg acggggttcg ccactaatta
     1861 attgattaaa atgcccgata gcacacacta gcgggcattt ttttagagtt cgcgcaacat
     1921 ccgcacttca caatcaacat ggcccgtgca gccaagtgca tagtcgatat gctcaaagcc
     1981 caaatgctca taaagcgcaa tggcttcctt taaaaagcgg tcgtttccag atagcagcgt
     2041 ttgaagccca tctctcgtgc ctgctccatc gccattaagg ccagtttttt cgccagccct
     2101 ttaccgcgaa tagccggaag aaaatacatc ttttgcagtt cgcaaatgtc cgattcacta
     2161 cctgctaatg gcgcaatccc accgccgccg accacttcac cctcgtactc aaccacccaa
     2221 tatgcatgac ccggctgact atacacttga tacagttcgt caagattcgg atcggcgacg
     2281 gtgtagcctt tatcagcggt aagaccgtat tcggcggata cctgacgaat gacgcgggca
     2341 atagcaggat tatcctgtag cgtcaggcga cgcattacag gtgattgcgg cgcaatgtta
     2401 ttcatagttt aacccaataa aaataaagta attatacatt tgtttaatac cactctcttc
     2461 ttacgtgcgc aagcatctta taaagcaata aagtttttgc cagcctatta ctgataagag
     2521 tattgtaacg tggcagaaca ttttactgtc tgtatatata aattatatca atggattttt
     2581 taattcaggg aatttttatg gctcaagtta ttaatgaaat ggatgttccg tcccattcgt
     2641 ttgtttttca tggtacaggt gagagatatt ttcttatttg tgtggtgaat gtgttgttaa
     2701 cgattataac gctaggtatc tatttaccat gggcattaat gaaatgtaag cgttatctct
     2761 atgctaatat ggaagttaac ggacaacgat tttcggtaat gctgccaact tactgattta
     2821 gtgtatgatg gtgattttaa ggtgcttgcg t
//
LOCUS       DAJTNN010000281         2832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTNN010000281 DAJTNN010000000
VERSION     DAJTNN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.287"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..136
                     /locus_tag="OO150_004079"
                     /pseudo
     CDS             1..136
                     /locus_tag="OO150_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            386..1252
                     /gene="ospB"
                     /locus_tag="OO150_004080"
     CDS             386..1252
                     /gene="ospB"
                     /locus_tag="OO150_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5518020.1"
                     /translation="MNLDGVRPYCRTVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1582..2322
                     /gene="phoN2"
                     /locus_tag="OO150_004081"
     CDS             1582..2322
                     /gene="phoN2"
                     /locus_tag="OO150_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5518021.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(2675..2832)
                     /locus_tag="OO150_004082"
                     /pseudo
     CDS             complement(2675..2832)
                     /locus_tag="OO150_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
BASE COUNT          929 a          515 c          525 g          863 t
ORIGIN      
        1 agcggtaaga gaagttcatc atacttggcg cagttacgcg atgtttctgc tagaggtgat
       61 gccgtaaaga acggcagaac attaccggaa caagatagcg gtctacctgc actggtatca
      121 gacccggggc ttccccggat gatctccacg gtactttagg tactgtgtct atattatgta
      181 ggtaggatca attaactttg tagttgaaat tctttcattg aaataatgaa taaaaaagac
      241 aaaccgcatt aaacaatgcc agaatataat tgcatttttg ttccgttttt ttaaatcata
      301 gtatacaaag aagcttgcat actatatgaa aatataacac cctttactat gatgtttata
      361 aaacaatata tggagtcatg taggtatgaa tttagatggt gttagaccat actgtagaac
      421 agtcaataaa aagaatgaaa gcatatcaga tattgcattt gcacatataa taaaaagggt
      481 aaaaaattca tcatgtactc acccaaaagc agcattggtt tttttaggag agaaaggttt
      541 ttgtgatagc aatgatgttc tatctattat gggacaacaa ataccaagag tatttaagaa
      601 caagatgtta tatgattatg tttttaaaaa tgaaaaaagt aaaaatgatt ttctaaaaat
      661 ggctgaatca tggctaccac agagtgaacc aatagtaata aataatgatg atgacgcatt
      721 gaatgctgct gcttattttt ctgtaaaaaa agcgaaaata aaaacagtaa acgatactga
      781 ttttaaagag tataataagg tttatattct tgggcacggt agtcctggtt ctcatcaatt
      841 aggccttggt tcggaactta ttgatgtaca aacaatcatt tcaagaatga aagactgtgg
      901 tattctaaat gtgaaagata tccgttttac ttcatgcggc tccgctgata aagtggctcc
      961 taaaaatttt aacaatgccc ctgctgaaag tctttcttgt atccttaact ctctgccttt
     1021 ttttaaggaa aaagaatctt tgctagagca gataaaaaaa caccttgaaa acgatgagtc
     1081 attgagtgat ggtctaaaaa tatccggcta tcatggatat ggagttcact atggtcaaga
     1141 gctttttccc tactcacatt atcgttcaac ttcaattcct gctgatccgg agcatacagt
     1201 aaaaagaagc tctcagaaaa agacttttat tattaataaa gaactggatt agtataaaat
     1261 ttttaaccta tagttaattg tttgcattta agatgaagtt atttttttct ttagtagaaa
     1321 agtgcttccc tatgagggaa aatcacaaat catcataata cagagacaaa acgatacgaa
     1381 caaatagata aaaaacatca ttcttttact acattatttt tcgtgaatat gaaaaataat
     1441 gttattactt taatataaga ctattttttg tttttccatc actctgttca aatttttccg
     1501 catgacttgt gttttttgta acacagctcg ttttttacag ctgaccaaaa tcatcaatta
     1561 attatgctaa ggaaataaat tatgaaaacc aaaaactttc ttcttttttg tattgctaca
     1621 aatatgattt ttatcccctc agcaaatgct ctgaaggcag aaggttttct cactcaacaa
     1681 acttcaccag acagtttgtc aatacttccg ccgcctccgg cagaggattc agtagtattt
     1741 caggctgaca aagctcatta tgaattcggc cgctcgctcc gggatgctaa tcgtgtacgt
     1801 ctcgctagcg aagatgcata ctacgagaat tttggtcttg cattttcaga tgcttatggc
     1861 atggatattt caagggaaaa taccccaatc ttatatcagt tgttaacaca agtactacag
     1921 gatagccatg attacgccgt gcgtaacgcc aaagaatatt ataaaagagt tcgtccattc
     1981 gttatttata aagacgcaac ctgtacacct gataaagatg agaacatggc tatcactggc
     2041 tcttatccct ctggtcatgc atcctttggt tgggcagtag cactgatact tgcggagatt
     2101 aatcctcaac gtaaagcgga aatacttcga cgtggatatg agtttggaga aagtcgggtc
     2161 atctgcggtg cgcattggca aagcgatgta gaggctgggc gtttaatggg agcatcggtt
     2221 gttgcagtac ttcataatac acctgaattt accaaaagcc ttagcgaagc caaaaaagag
     2281 tttgaagaat taaatactcc taccaatgaa ctgaccccat aaagctggac agcctgtatc
     2341 aggctatgga gggcccatag acaaatctac cctatatgag cataggagga gtctatgggc
     2401 acaccacgtt ttacccctga atttaaggga ttactggaaa ggctgggaca tatccctccg
     2461 gcagaagcag aaaaagctta ttatgctgcc atcggaaacg atgatctggc aacctgagtt
     2521 cacagataaa acattctcta ggaaactcgg ggcggttccg ttcaccacat gcaatgtggt
     2581 gttgcagggg aacggtctgc accatcccct atgtcgatca atataacaga aatgacaact
     2641 tcagattcag ggcacaacct aaatatattt tagatcacct ctcaaatcgt ttgcctgata
     2701 ccgctccatt ttttaataaa aaaatcaatc atttttgaat ctttgctttt cattgcattg
     2761 tcaagcatag tgtccccact atttgctttt tgaaattttt tgtttacatc aaccaatcca
     2821 ttattgataa aa
//
LOCUS       DAJTNN010000282         2783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTNN010000282 DAJTNN010000000
VERSION     DAJTNN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.288"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..579
                     /locus_tag="OO150_004083"
     CDS             <1..579
                     /locus_tag="OO150_004083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518022.1"
                     /translation="KKNNVPMIQNNGNLQPFSMGNLPSIAKTFPMEIHTTILQQLNPL
                     EHISNAISRICYYKEQCKALELESLKIKEQASFMHLKIDTNFKKAMYELETRRKSNEL
                     KLIAAANDFKQISITKSDLIKEISKLVSSICSNDVSLESKKAMNDTLIIMKGMLSDQR
                     SESYEKFKSLLSSIDSSYNDALLIEDHQDNYV"
     gene            572..1153
                     /locus_tag="OO150_004084"
     CDS             572..1153
                     /locus_tag="OO150_004084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518023.1"
                     /translation="MFNIILGVAAVGVGLYILNELDDRAGEEKKRWENQRESVCKSIE
                     EHKRYIEQHVEYVQSMYDFHQLVDLHYSCMKVANEAYKLLNDAKKSMGAIYDAINKLK
                     AHRSKLFNDKKESDDYEKKKEIQAEINMIKEIIDGLYSELDLIKKDKEHFYQELKAFN
                     LKTSSIKYRIRDNTGYKGFDWYNRLQERTLLRR"
     gene            2133..2609
                     /locus_tag="OO150_004085"
     CDS             2133..2609
                     /locus_tag="OO150_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5518024.1"
                     /translation="MFIDSEKRLKQLSDEAKKSTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          906 a          545 c          624 g          708 t
ORIGIN      
        1 aaaaaaaata acgtgccaat gatacaaaat aatggaaatc tccaaccatt tagcatgggt
       61 aatttaccat ctattgctaa aacattccca atggaaatcc atacaactat acttcaacag
      121 cttaacccat tggagcatat atccaatgct ataagtagga tttgttacta taaagaacag
      181 tgtaaagcac ttgagcttga gtcactaaaa ataaaagaac aagccagttt tatgcacctt
      241 aaaatagata ctaattttaa aaaggcaatg tatgagctag aaacaagaag aaagagtaat
      301 gagttgaagt tgatagctgc tgcaaatgat tttaagcaaa tatcaataac aaaatcagac
      361 ttaattaaag agatatcaaa gcttgtttca agcatatgta gcaatgacgt ttcgttggaa
      421 agtaaaaagg cgatgaacga tactcttatc attatgaaag gcatgctcag tgatcaacgg
      481 tctgagtcat atgaaaaatt caaatctttg ttaagtagta ttgattctag ttacaatgat
      541 gcgttgttga tagaggatca tcaggataat tatgtttaat atcatactcg gagttgctgc
      601 tgtaggtgtt ggtttatata tcttgaacga acttgatgat cgcgccggag aagaaaaaaa
      661 gcgatgggaa aatcaacgag aatcagtatg taaaagcatt gaagaacata agagatatat
      721 agaacaacat gtcgaatatg ttcaatcaat gtatgatttt catcagttag ttgaccttca
      781 ttattcatgt atgaaagtgg caaatgaagc atataaactt cttaatgatg ctaaaaaatc
      841 aatgggcgct atttatgatg ctatcaataa actcaaagct caccgctcta aattatttaa
      901 tgataaaaaa gaaagcgatg attatgaaaa gaaaaaagaa atacaagcag aaataaacat
      961 gatcaaagaa atcatagatg gcctatactc tgaattagat ttaattaaaa aggataaaga
     1021 acatttttat caggaattaa aagcttttaa cttaaaaact agcagcatta aatacagaat
     1081 tagagacaat accggatata aaggctttga ttggtataat cggttacaag aacgaacttt
     1141 acttcgacgt tagttttgca ctgaaactat atcagataac aatcctccac ggttccggca
     1201 gcgccttgag cgcaaccgga aaccgtctgg ggagaacccc agacccccat cgctgcaaca
     1261 tcgacggtct gcaccacctg ccaaccgagt aactgcctgt tccgtgcgaa tgggctttgc
     1321 agggggctgg cgcggttcct ggcgacctga aagcccctgg ttaggggtat cggcgcgcct
     1381 gacctcctga gagcctctgt aacgcatttt cgcgcctccc atgccctggc gggcatttaa
     1441 aacccgtttc gtgcgtctga gagcgtttga agggctatct gacgactcat ccccgaaaga
     1501 acaacgacgg aatcaccgac aacatgcgag aggtggcacc gctcgccgca gccccagcga
     1561 ccgaacgtta gtgagcgagt gggcgaggaa gcggaagaga accggtagtt agattgagca
     1621 cgtacgcact gatgggtgtc ggggcgcagc cctgaccaga tggcaaacgg aacatcgtcg
     1681 tgggtgacgg tattacattt gcacatcctg tcccgatttt tgaggcattt tagccttcat
     1741 ccccctttta gtggtgaacg gacggcaaaa acatgatgga actgttgaac gtggagagcg
     1801 cgaaatcgga caaaaatcac ggttgacgag gcttgcagag gagtaagcca gtatacactc
     1861 cgctatcgct acgagactgg gtcagggctg cgccccgaca cctccaaagg gtgttgcgtt
     1921 gagcgcaaca cccttgccta gaatagatct tttacgccga tttgtaagtt gttgtttttt
     1981 tagtgtgttt tttgtttacc tgttgcatcc agggtgacac aacgttgtcg catcctggat
     2041 gcgacaacac gcaacaaggt gttgcacaga tgtatcatga tggcttagat ttgaaccaac
     2101 gttagtgcat gggattttca gagggaaaaa tcatgtttat tgattcagaa aaacggctga
     2161 aacaactttc agatgaggca aagaaaagca ccgaggatct cgaagaagca aagaagaatt
     2221 caaggtttac acaggtatcc ccaaaaggtt gggaacgcgt tcgagagctg ctgaaggata
     2281 gccaaggcat atcagcactg aagctgtact catttttagc ggagcatatc gatccaacgt
     2341 gtggcgctgt cgttgcggat cagcaattcc tagctgaaaa acttggagtt agcagaagca
     2401 caattattcg gtggctcaat tacttagaat caaaaaatgc attagttaga atccccgttg
     2461 ctggtaaggt ttgtgcgtat gccctcgatc cacatgaagt ctggaaggga tacaacacta
     2521 cgaaaaacca tgcagcgttt gtcactaaaa cactggtcaa caaagacggt gatattcagc
     2581 gccgaatcat ggccatgttt tcaaattgag ccagcggcag gcggacaatc aggggctacg
     2641 tgttaacgtt ttgaccatga ttgtctatcc tgcattgctc ttttaccacc tagacagcac
     2701 catagtgata agataaacat gctcaaacgt tttatgtaac attagaaaaa ttcagttaat
     2761 taaaaaaggt tttctaaatg agc
//
LOCUS       DAJTNN010000283         2750 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTNN010000283 DAJTNN010000000
VERSION     DAJTNN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.289"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..2552
                     /locus_tag="OO150_004086"
     rRNA            <1..2552
                     /locus_tag="OO150_004086"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2648..>2750
                     /gene="rrf"
                     /locus_tag="OO150_004087"
     rRNA            2648..>2750
                     /gene="rrf"
                     /locus_tag="OO150_004087"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          713 a          606 c          865 g          566 t
ORIGIN      
        1 catattgtga gctcgatgag tagggcggga cacgtggtat cctgtctgaa tatgggggga
       61 ccatcctcca aggctaaata ctcctgactg accgatagtg aaccagtacc gtgagggaaa
      121 ggcgaaaaga accccggcga ggggagtgaa aaagaacctg aaaccgtgta cgtacaagca
      181 gtgggagcac gcttaggcgt gtgactgcgt accttttgta taatgggtca gcgacttata
      241 ttctgtagca aggttaaccg aataggggag ccgaagggaa accgagtctt aactgggcgt
      301 taagttgcag ggtatagacc cgaaacccgg tgatctagcc atgggcaggt tgaaggttgg
      361 gtaacactaa ctggaggacc gaaccgacta atgttgaaaa attagcggat gacttgtggc
      421 tgggggtgaa aggccaatca aaccgggaga tagctggttc tccccgaaag ctatttaggt
      481 agcgcctcgt gaattcatct ccgggggtag agcactgttt cggcaagggg gtcatcccga
      541 cttaccaacc cgatgcaaac tgcgaatacc ggagaatgtt atcacgggag acacacggcg
      601 ggtgctaacg tccgtcgtga agagggaaac aacccagacc gccagctaag gtcccaaagt
      661 catggttaag tgggaaacga tgtgggaagg cccagacagc caggatgttg gcttagaagc
      721 agccatcatt taaagaaagc gtaatagctc actggtcgag tcggcctgcg cggaagatgt
      781 aacggggcta aaccatgcac cgaagctgcg gcagcgacgc tgatgcgttg ttgggtaggg
      841 gagcgttctg taagcctgtg aaggtgtgct gtgaggtatg ctggaggtat cagaagtgcg
      901 aatgctgaca taagtaacga taaagcgggt gaaaagcccg ctcgccggaa gaccaagggt
      961 tcctgtccaa cgttaatcgg ggcagggtga gtcgacccct aaggcgaggc cgaaaggcgt
     1021 agtcgatggg aaacaggtta atattcctgt acttggtgtt actgcgaagg ggggacggag
     1081 aaggctatgt tggccgggcg acggttgtcc cggtttaagc gtgtaggctg gttttccagg
     1141 caaatccgga aaactaaggc tgaggcgtga tgacgaggca ctacggtgct gaagcaacaa
     1201 atgccctgct tccaggaaaa gcctctaagc atcaggtaac atcaaatcgt accccaaacc
     1261 gacacaggtg gtcaggtaga gaataccaag gcgcttgaga gaactcgggt gaaggaacta
     1321 ggcaaaatgg tgccgtaact tcgggagaag gcacgctgat atgtaggtga agtccctcgc
     1381 ggatggagct gaaatcagtc gaagatacca gctggctgca actgtttatt aaaaacacag
     1441 cactgtgcaa acacgaaagt ggacgtatac ggtgtgacgc ctgcccggtg ccggaaggtt
     1501 aattgatggg gtcagcgcaa gcgaagctct tgatcgaagc cccggtaaac ggcggccgta
     1561 actataacgg tcctaaggta gcgaaattcc ttgtcgggta agttccgacc tgcacgaatg
     1621 gcgtaatgat ggccaggctg tctccacccg agactcagtg aaattgaact cgctgtgaag
     1681 atgcagtgta cccgcggcaa gacggaaaga ccccgtgaac ctttactata gcttgacact
     1741 gaacattgag ccttgatgtg taggataggt gggaggcttt gaagtgtgga cgccagtctg
     1801 catggagccg accttgaaat accacccttt aatgtttgat gttctaacgt tgacccgtaa
     1861 tccgggttgc ggacagtgtc tggtgggtag tttgactggg gcggtctcct cctaaagagt
     1921 aacggaggag cacgaaggtt ggctaatcct ggtcggacat caggaggtta gtgcaatggc
     1981 ataagccagc ttgactgcga gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga
     2041 tccggtggtt ctgaatggaa gggccatcgc tcaacggata aaaggtactc cggggataac
     2101 aggctgatac cgcccaagag ttcatatcga cggcggtgtt tggcacctcg atgtcggctc
     2161 atcacatcct ggggctgaag taggtcccaa gggtatggct gttcgccatt taaagtggta
     2221 cgcgagctgg gtttagaacg tcgtgagaca gttcggtccc tatctgccgt gggcgctgga
     2281 gaactgaggg gggctgctcc tagtacgaga ggaccggagt ggacgcatca ctggtgttcg
     2341 ggttgtcatg ccaatggcac tgcccggtag ctaaatgcgg aagagataag tgctgaaagc
     2401 atctaagcac gaaacttgcc ccgagatgag ttctccctga ctccttgaga gtcctgaagg
     2461 aacgttgaag acgacgacgt tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta
     2521 accggtacta atgaaccgtg aggcttaacc ttacaacgcc gaaggtgttt tggcggatga
     2581 gagaaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     2641 gaatttgcct ggcggccgta gcgcggtggt cccacctgac cccatgccga actcagaagt
     2701 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
//
LOCUS       DAJTNN010000284         2645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTNN010000284 DAJTNN010000000
VERSION     DAJTNN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.290"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            21..857
                     /locus_tag="OO150_004088"
     CDS             21..857
                     /locus_tag="OO150_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5518025.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSY
                     MWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALNILVNAVINGSLGVGSTNALGGSS
                     IVLGDNDTGFKQNGDGILDVYANSQRVFRFQNGVAIAFKNIQAGDGKKFTLSSSNNST
                     KNATFNLWGASTRPVVAELGNEAGWHFYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYV
                     KDVRLGTRVVQLMARGGRYEKAGHAITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVA
                     QV"
     gene            826..1389
                     /locus_tag="OO150_004089"
     CDS             826..1389
                     /locus_tag="OO150_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5518026.1"
                     /translation="MAHGITSHRCKLCSILKNIKSGNPKTKEQYQLTKNFDVIWLWSE
                     DGKNWYEEVNNFQDDTIKIVYDENNIIVAITKDAPTLNPEGFSVVEIPDITANRRADD
                     SGKWMFKDGTVVKRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMAMDEERT
                     RLESWERYSVLVSRVDTANPEWPQKPE"
     gene            complement(1418..1951)
                     /locus_tag="OO150_004090"
     CDS             complement(1418..1951)
                     /locus_tag="OO150_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5518027.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYYHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1954..2511)
                     /locus_tag="OO150_004091"
     CDS             complement(1954..2511)
                     /locus_tag="OO150_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5518028.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNALIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGVELGWSDFYTT
                     TRKPSAGDVGAYTRAECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
BASE COUNT          755 a          567 c          586 g          737 t
ORIGIN      
        1 taaacagggc tggtaacgcc gtgcaaaaaa atggcgatac cttgtccggt gggcttactt
       61 ttgaaaatga ctcaatcctt gcctggattc gaaatactga ctgggcgaag attggattta
      121 aaaatgatgc cgatggtgac actgattcat acatgtggtt tgaaacaggc gacaacggca
      181 atgaatattt caaatggaga agccgccaga gcaccacaac aaaagacctg atgacgctga
      241 aatgggatgc actaaatatt cttgttaatg ctgtcattaa tggcagtctt ggagttggtt
      301 cgacgaatgc gttaggtggt agctctattg ttcttggtga taatgacacc ggatttaaac
      361 agaatggaga cggtattctg gatgtttatg ctaatagtca gcgtgtattc cgctttcaga
      421 atggagtggc tattgctttt aaaaatattc aggccgggga tggtaaaaaa ttcacgctat
      481 ccagctccaa caactccaca aagaatgcaa cgtttaattt atggggtgct tcaacccgtc
      541 cagtagttgc agagttaggc aatgaggcag gatggcattt ctatagccag cgaaatacag
      601 ataactcggt aatattttct gttaacggtc agatacagcc cagcaactgg gggaattttg
      661 attcacgcta tgtaaaagat gttcgcctgg gtacgcgtgt tgttcaattg atggcgcgtg
      721 gtggtcgtta tgaaaaagcc ggacacgcaa ttaccggatt aagaatcatt ggtgaagtag
      781 atggcgatga tgaagccatc ttcagaccaa tacaaaaata catcaatggc acatggtata
      841 acgtcgcaca ggtgtaaatt atgcagcata ttaaaaaata ttaagtccgg aaatccaaaa
      901 acaaaagaac aatatcagct aacaaagaat tttgatgtta tctggttatg gtccgaagac
      961 ggtaaaaact ggtatgagga agtaaataac tttcaggacg acaccataaa gattgtatac
     1021 gacgaaaata atattattgt tgccataacc aaagatgccc caacgcttaa tcccgaaggc
     1081 tttagtgtcg ttgagattcc agatataaca gccaatcgtc gtgccgatga ttcagggaag
     1141 tggatgttta aggacggaac tgtggttaag cggatttata cagcagacga acagcaacaa
     1201 caggccgaat cacaaaaggt cgcgttgctt tctgaagctg aaagcgttat tcagccactg
     1261 gaacgcgctg tcaggctgaa tatggcgatg gatgaggaac gcacacgact ggagtcatgg
     1321 gaacgctaca gcgttctggt cagccgtgtg gatacagcaa atcccgaatg gccacaaaag
     1381 cctgagtaaa aattaaggcc caatatcggg ccttctctca ttctggttgt tcgggaaacg
     1441 ttactggcag gctggaggtg tctgtagatt cgactttctg cgcatagagc atccactcgg
     1501 ttaatttttg tttattctcg tcggaaatga tgcccagccg tagctgtgag tcccatagct
     1561 gggttttatc cctgacaagt tgcaacaggc tttgcttttc attttccgct tgttgcctct
     1621 gctcttcctc ggtataagtt cgctttatca ctacgccatc tttgaacaac catttccccg
     1681 aaatatcagc ccggcgattt gctgtaatat caggtaattc aacgacgctt gcgccttccg
     1741 gattaattgc tgaaacatcc ttttcaatac aaataataac gccgttatgg taatagacca
     1801 ttttcaaagt gtctggctgg aaattctttt gttcctcata ccagtttttc ccatcatctg
     1861 aataaagcca tttgatgtta aattgctttg ttagctggta ttgctctttt gttttagggt
     1921 tgccagcagt aatgtttttt aagtgcatca tcgttaaata ctccccgcgt tataccacgt
     1981 cccattaatg caatactgaa ttggccttgc ctgtgttgta tcaattaatt catcacggtt
     2041 tccgttaact gaacccgtaa cgacataacc tgacctgtca gaccagccgg gaccattcca
     2101 tgtctgaaca gatgacagac cgccaaggcg aatacctgta ataaaccttg agttacattc
     2161 tgcccgcgta tatgcaccaa catctcccgc tgaaggtttt cgtgtcgtgg tatagaaatc
     2221 tgaccaacct aattcaaccc cataaccatc acgagccgac ctgtaggaaa tgccaccatt
     2281 tttataattc acacggaatt gtacggcagg acagctacca gcatttatat taaagtgaag
     2341 aattaatgca gatgcaccac caatcagggc gttatatgca ccactatccc aattccatcc
     2401 aacggatgta tcaccattaa cagtattacc tgtttgtcgg agtgcaaatg cattgacatt
     2461 tttggcacta attgaaatat cgctagaacc atcaaaacca actccagcaa tttttcgtgc
     2521 tgttttcagc tttgttgcag tagcggcatt gccggacagt tccccagaaa ggccaccgct
     2581 gaatgtttgt cgattggtcc aggtattcgc tgtactgagt aacggtattt tctccccgct
     2641 tgtgc
//
LOCUS       DAJTNN010000285         2558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTNN010000285 DAJTNN010000000
VERSION     DAJTNN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.292"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..356
                     /locus_tag="OO150_004092"
     CDS             <1..356
                     /locus_tag="OO150_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5518029.1"
                     /translation="KPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQKAG
                     IASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVS
                     RRDQESALACWDIDR"
     gene            512..694
                     /locus_tag="OO150_004093"
     CDS             512..694
                     /locus_tag="OO150_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518030.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            1021..1173
                     /locus_tag="OO150_004094"
     CDS             1021..1173
                     /locus_tag="OO150_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001228697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCR5518031.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPEWIMQPAPDWQTPLNGII
                     SPSESG"
     gene            1259..1483
                     /locus_tag="OO150_004095"
     CDS             1259..1483
                     /locus_tag="OO150_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518032.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            1828..2154
                     /locus_tag="OO150_004096"
     CDS             1828..2154
                     /locus_tag="OO150_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCR5518033.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(2286..2486)
                     /locus_tag="OO150_004097"
     CDS             complement(2286..2486)
                     /locus_tag="OO150_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR5518034.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
BASE COUNT          629 a          554 c          693 g          682 t
ORIGIN      
        1 gtaaacctgt cgtcccgggc atgaagttgt cgaaggaaaa atgcgaccag gtcaacgcca
       61 ttgagcgtga taaggcgctg gcatgggtgg agcgcaatat taaagtgccg ctgaccgaac
      121 cccagaaagc gggtatcgcg tcattttgtc cctataacat tggccccggt aagtgtttcc
      181 cgtcgacgtt ttacagacgg attaatgctg gtgaccgcag gggagcatgc gaagcgattc
      241 gctggtggat taaggacggt ggcagagact gccgtatccg ctcaaataat tgctacggtc
      301 aggtatcccg gcgagaccag gaaagtgcgc tggcgtgctg ggacatcgac agataagcag
      361 aatattttgc tgaaaataag gcatgagcac gcggacggat aacacgaaat cctgcgaact
      421 ggcgaaacgt aagtgaataa aagtaaaaac cccgtttgtt ggcaccaggc ggggttttgt
      481 gtttctgacc ttgagtaagg caagggagaa catggcgaag tataaacgaa ttctgttgag
      541 gttgaccatg aaaaacggcc ttgaactgaa agcgcctgta actgatgaca tcagcagagc
      601 actggctttt gccattaagt gggtggcggt cggtgttgct gtgtccccga tgctgtatgg
      661 gctggcaaaa ctggtcattg cgttgaaatc gtgaagggag gattaagcat gtcagacaaa
      721 ctcataacgc tggcgaagat cctctgtgta attgtcggca tttcattttc actaatgctg
      781 gttgctcttt ttctttccat ggcctggatg atgttgtctt cgtcggggct gctggggtga
      841 acataaaccg aatgctttcc gcgtttaccg ttattctgct ggtggtctgt ggtgcgctgt
      901 ggctggcaac agaccattac cgtgataacg ccatcaccta caaagcgcag cgcgataaaa
      961 aagccagaga gctggagctg gcaaacgcaa ccattactga catgcagcag cgccagcgtg
     1021 atgttgccgc tggtcgtaag cgcctgcgga tcaacgccac ctgctccggt accgtgcgtg
     1081 aagccaccgg cacctccgga atggataatg caaccggccc ccgactggca gacaccgctg
     1141 aacgggatta tttcaccctc agagagcggt tgatgacaat gcagaagcaa ctggaagggg
     1201 cgcaggaata tatccgcact cagtgtatta actagtattt ttgttatccg gagaatgcat
     1261 gaagaaatta ctggtaaccg taaagccttt tcagggaaca attccgttcc gtattttgca
     1321 gcgtggtcgt gttcttgttg aaggttcgtt cagtggtaaa tgtacgcaat tacactcccg
     1381 gacctttcag gtgaatgcca cgaatgaaga gctaaccgtt gagtgtacga tgaatgccgc
     1441 taaatgccgc atggtatccg ctgcattaca gccagtgtgt tgagcgacct tattaaccat
     1501 gcgcggtatt gtcgccgtat ccccgcatta acagagaccg cagcccgacc gggagactcc
     1561 tctgcgcgag tgtgcgggga taatcaaaaa cgatacacac cggggtttac cgcgttaacg
     1621 gagcgcggcg ttgtcccctc atggtcgctg gtccggtgcg atggtggaag aaaccggacg
     1681 atgtgttacc tcgcaagctc tgttatgtca tgtgtctgat ttgtgattta agtcggataa
     1741 ttgtcgttgc cattaagcag aggattgatg accgacaggg tggcattgtt agaataagac
     1801 ttattcttat ctgtgccggg aatgaaaatg aaaagaaacc ttccgttaat tattttgttg
     1861 tcttctctgg ttatgggctg tacgcaacat aaaacagata tgccccgaca gttggttaag
     1921 gcattaccac aatatccggc ctatgcagcg gcaaattata taaagggacg ggttgatgtg
     1981 aagtttgata ttggtgctga tggtactgtc acccgaattg agtttatccg ttcagagccg
     2041 caccatttgt ttgatgagca ggttgtaaag gcgatggcaa aatggcgatt tgagaaggac
     2101 aggccgtgta aaggcgtgaa gaaaacgttt atctttagtc cttctgcacc ctgattattt
     2161 catcagaaat taattatcac tctgttgtta ttctgtacat cccggcaggg taagtcttgt
     2221 tccgtcggat atgaagatga aatattgttg gaggacagtg ggtacctgct cctgtaaccg
     2281 aacgttcatt tctcgttatt tttcatgctg gccgggcgca gatgcgttgc atctgttgcc
     2341 agccttctcc tgcaggcttc aataacccac gctgaaaagt ttcctgaacc tttcagatca
     2401 agagcgatgt taatttgttc aatcatctgg tttggaaatc ggatgttgcg ggttgttgtt
     2461 ctgcgggttc tgttctttga tgacataatg tttccccata ttcagtgttg ctgatttgta
     2521 ttatctgaag ttgcttttac gttaatttga tgcagatc
//
LOCUS       DAJTNN010000286         2552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTNN010000286 DAJTNN010000000
VERSION     DAJTNN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.293"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..2466)
                     /locus_tag="OO150_004098"
                     /pseudo
     CDS             complement(1..2466)
                     /locus_tag="OO150_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          593 a          639 c          622 g          698 t
ORIGIN      
        1 ggggacaggt tgaacctctg cccggcttct agggagatgc tcattccatt cgcagtgccg
       61 ttggcgttaa cgccgttgtt atgactgtcc agtacgtgga aactattttt ctggcgcgat
      121 gcttttataa cgagatcgct gtaaaaaccg ttttgtgcca tgtaactggc gtacattccc
      181 atgtagtctg aacgtgcggt accgtcgcct ccgctatagt ccggcgatgc atgtgttgag
      241 ccaatataca gaccgacata caacggcatt tcatcagaga gacgtttatc cccaccaaac
      301 tggataccgc tgtaacccat gtcaaagccg ctcagtttgc cactggcaaa ggagtccagg
      361 cttcccccat aactgcgcaa ccagatatta ccgtctttac tctgatttcg caggtcaccc
      421 atccgttgca tcagcgtacg gttttcaaca tagttcaata agtaaccgac attgagataa
      481 ttgccgccag catctgcggt cgtggtgggt ttaggcgtgg gagcgggttc aggcgtagga
      541 tcggggttga ctatgggagg ctgagcggga gctggtgtgg gttcaggatt aggagtcggt
      601 tccggaactg tcccggaagc gtaaagctcc cagttagtgc catttttacg cacatcgtac
      661 agatatcccc cccaactcaa cctgagaaga cgcgctgaac gaggccgcgc cgtcagtggt
      721 ttttaccact gtcagaactt catttcccgt tgtggcctcg ctgccctggt tgcggatagc
      781 caatacgtga ttaccagcac tactcccgct gatattcaat aaatcccctt tattattaac
      841 gccattaccc tcgccaacaa catcagcacg cataataaag gtactgttac cgctcaggtt
      901 ctctacgttt aatgtggcaa atgtgccggc agttgacccg tggctggcaa aatcgacagt
      961 tgaatggctc agcgccagcg tgtcgagatt agaattactt gttacgttcc agacgctgtt
     1021 attcattgca acgtccagtt ttccgccatt gacattatcg ctgagggagg aacctgtcca
     1081 aaccgaccca gggtgcatat ccagattgat tagcccacct ttggaaaaaa tgctaccgtt
     1141 gataagcata cgacccgagg cgttgatgcg gctggcggca taaccatcgt catgttgcgt
     1201 tgcaatggcc atcttgtcgg gtgtactcat atcaatgacc agatcgctcg tgaggtcgat
     1261 ctggctgttg gtcatggcat aaattcccct ggctcctgcc gcgccggtga tagccaaact
     1321 gtctccggtt attctaccgc cgctgagcgc ccacaaaccc agcgccagac tgccattacg
     1381 atcaactgta atatcggtat tttgcatgtt gacaacagcc gttgccgtct gggccgaggc
     1441 accataagaa ccgccggaaa agatgctgtt tcgttgcgct gccgtgccag aaaaattgat
     1501 tgtcgcgtct gaaccactgg tgacgaggcc accgccctgc gcggaagaaa tatggctatc
     1561 tgcaccgata gtggttgtac caccacgaac ttcgacgcca ttggccgcgg ctccagttac
     1621 atcaacagtg agtgcattcg cgctaacctg gccaaagctc cagaggccgt gtgcattatc
     1681 gccattggtt ttaatggaac tgtttgttcc gagatcgaca acagagtttt tctgtacgtt
     1741 tatccccatg gcgctgtagc cctgaacatc gattgtcagg tctgtagccg taaaacgcgc
     1801 agcaccattg gcgttattgc cgttgagacc accgatataa acacctgtac ttccatcggt
     1861 cgtgatttta cttccgcttc caagatcgac actggtgcca tagtcattga tggttaggcc
     1921 tataccgttc gagttttcaa tggtgaattg agtggctgtt agtgttgagc tatgcccaat
     1981 aatgatgccg tcatcgttac tcttaatggt gctgcctgtg cctaagtcag catgggtata
     2041 gtcaccaatt aagttaatgc cgatggcaga ggtttgacca acaacatcta ctgtgagtcg
     2101 gttagctgtc agggagctgt ttttgccctg aataatgatt ccttttgcat atccagaggc
     2161 gtcgttgaca ttgacggtga catcattacc caggttgata ttgccaggcg tgtttcctgc
     2221 tggggtcatt acaccataga ggtttcctga tggatcggcg gtatctcctg taattctgtc
     2281 accatcatta atagtaatat tggtgccata ttgacttcct gtatcagcag ctatcagcag
     2341 gggggaaaag ataattgcat tcaaaccaat agcgccaaat tttatcagtt gaattgaggg
     2401 cgaacggatt tttaattcag cgagacgatc tgctttggta ttcccattgt gttgtttgat
     2461 gcccatacca ctcctatata gtacccagac tatgggtcta tggattttgt cattttgttt
     2521 aaatcaatga ataatatcct cttatcatca tg
//
LOCUS       DAJTNN010000287         2531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTNN010000287 DAJTNN010000000
VERSION     DAJTNN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.294"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1533
                     /locus_tag="OO150_004099"
     CDS             <1..1533
                     /locus_tag="OO150_004099"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR5518035.1"
                     /translation="AEDLGITCALIPTTTPGVEIGRRHFPLNVPFQNGPTRGKDVFVP
                     IDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALATGAYAHIRRQFKIS
                     IGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLLAIVKYHCTHRGQQSII
                     DAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMIFGQGAIRCHPYVLE
                     EMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSSTPTGDATKRYYQHLN
                     RLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAVLKRYDDEGRNEADL
                     PLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRHYLAPSDKLDHKVAK
                     ILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQRICKELGKNLPFTR
                     LDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELATKPVKLPEKVRKVE
                     AA"
     gene            complement(1576..2022)
                     /gene="ivy"
                     /locus_tag="OO150_004100"
     CDS             complement(1576..2022)
                     /gene="ivy"
                     /locus_tag="OO150_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5518036.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            2203..>2531
                     /gene="yafV"
                     /locus_tag="OO150_004101"
     CDS             2203..>2531
                     /gene="yafV"
                     /locus_tag="OO150_004101"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5518037.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHL"
BASE COUNT          583 a          655 c          690 g          603 t
ORIGIN      
        1 gcagaagatt taggtattac ctgtgcgctg atcccgacca ccacgccggg cgtggaaatt
       61 ggtcgtcgcc acttcccgct gaacgtaccg ttccagaacg gaccgacgcg cggtaaagat
      121 gtcttcgtac cgatcgatta catcatcggt gggccgaaaa tggccgggca aggctggcgg
      181 atgctggtgg agtgcctctc ggtaggccgc ggcatcaccc tgccttccaa ctcaaccggc
      241 ggcgtgaaat cggtagcgct ggcaacgggc gcgtatgctc acattcgccg tcagttcaaa
      301 atctctatcg gaaagatgga agggattgaa gagccgctgg cgcgtattgc cggtaatgcc
      361 tacgtgatgg atgctgcggc atcgctgatt acctacggca ttatgctcgg cgaaaaacct
      421 gccgtgctgt tggctatcgt taagtatcac tgtacccacc gcgggcagca gtcgattatt
      481 gatgcgatgg atatcactgg tggcaaaggc attatgctcg ggcaaagcaa cttcctggcg
      541 cgtgcttacc agggcgcacc gattgccatc accgttgaag gggctaacat tctgacccgc
      601 agcatgatga tcttcggaca aggagcgatt cgttgccatc cgtacgtgct ggaagagatg
      661 gaagcggcga agaacaatga cgtcaacgcg ttcgataaac tgttgttcaa acatatcggt
      721 cacgtcggta gcaacaaagt tcgcagcttc tggctgggcc tgacgcgcgg tttaaccagc
      781 agcacgccaa ccggcgatgc cactaaacgc tactatcagc acctgaaccg cctgagcgcc
      841 aacctcgccc tgctttctga tgtctcgatg gcagtgctgg gcggcagcct gaaacgtcgc
      901 gagcgcatct cggcccgtct gggggatatt ttaagccagc tctacctcgc ctctgccgtg
      961 ctgaagcgtt atgacgacga aggccgtaat gaagccgacc tgccgctggt gcactggggc
     1021 gtacaagatg cgctgtatca ggctgaacag gcgatggatg atttactgca aaacttcccg
     1081 aaccgcgtgg ttgccgggct gctgaatgtg gtgatcttcc cgaccggacg tcattatctg
     1141 gcaccttctg acaagctgga tcataaagtg gcgaagattt tacaagtgcc gaacgccacc
     1201 cgttcccgca ttggtcgcgg tcagtacctg acgccgagcg agcataatcc ggttggcttg
     1261 ctggaagagg cgctggtgga tgtgattgcc gccgacccaa ttcatcagcg gatctgtaaa
     1321 gagctgggta aaaacctgcc gtttacccgt ctggatgaac tggcgcacaa cgcgctggcg
     1381 aagggcctga ttgataaaga tgaagccgct attctggtga aagctgaaga aagccgtctg
     1441 cgcagtatta acgttgatga ctttgatccg gaagagctgg cgacgaagcc ggtaaagttg
     1501 ccggagaaag tgcggaaagt tgaagccgcg taattgaata acggagccga aaggctccgt
     1561 ttctttatcc gctaattatt taaaattaaa gccatccgga tggttttcca ggctgccggt
     1621 caacgccgcg aacagcaccg ttttaccatc aatcgaaagc gcatcgttca cattcagcca
     1681 ggtgagtttc tcttgcgacg ttttctcatc aatagtcgag aacagccccg tcatctgatt
     1741 agatttctcg gaccacatca cagcgatacg ttgcgagcca cagtcatgcg gtttgcacgc
     1801 gctcatcacc tgatacgtct catctcccaa cgttacggtt tgtgcgggag tataagtacc
     1861 gcctttcatc acccaggcag gcagcttatg cccttgtacc atctgattaa atgcagcttt
     1921 ggtggtttcg ccctttgcaa ggctgctaat ggttaaatca tcctgcgcca ttgcactggt
     1981 ggcgatgacc agagcggcga ctgtcgttat tgccttaaac atcattcctc ccgagcttat
     2041 cctgcccatg ttattaacct tctaaagcct ggcgataaca cgataatttg caaatcagat
     2101 atttccaata tcagcatttg ttatcaattt attctgcgct aaggcaactg cctgataagg
     2161 tgttaaagtt ataaaaccct tcgcataaag gagttgtaca ccgtgcctgg tttgaagatt
     2221 acgcttttgc agcaaccact ggtgtggatg gatggccctg ccaacctgcg tcattttgat
     2281 cgtcaactgg aaggtattac cgggcgcgat gtgatcgttc taccggagat gtttaccagc
     2341 ggctttgcca tggaagcggc agcttcgtcg ctagcacaag atgacgtagt gaactggatg
     2401 acagctaagg cgcagcagtg caatgcgctg atagcaggca gtgttgcatt acaaacggag
     2461 tctggttcgg ttaaccgctt tttgctggtt gagccgggcg gcacggtaca tttttatgat
     2521 aagcgtcatc t
//
LOCUS       DAJTNN010000288         2525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTNN010000288 DAJTNN010000000
VERSION     DAJTNN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.295"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..165)
                     /locus_tag="OO150_004102"
     CDS             complement(<1..165)
                     /locus_tag="OO150_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5518038.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTL"
     gene            complement(231..575)
                     /locus_tag="OO150_004103"
     CDS             complement(231..575)
                     /locus_tag="OO150_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCR5518039.1"
                     /translation="MTEADLYPHLAHLAGGQVSPYVAPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(572..1018)
                     /locus_tag="OO150_004104"
     CDS             complement(572..1018)
                     /locus_tag="OO150_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCR5518040.1"
                     /translation="MIEILLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRTPVRRGKLRRNVVILSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(1015..1335)
                     /locus_tag="OO150_004105"
     CDS             complement(1015..1335)
                     /locus_tag="OO150_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5518041.1"
                     /translation="MISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGRSSRERMQSGA
                     EMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDATGGRLEILCRL
                     GGEK"
     gene            complement(1375..1701)
                     /locus_tag="OO150_004106"
     CDS             complement(1375..1701)
                     /locus_tag="OO150_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5518042.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVLREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1698..2297)
                     /locus_tag="OO150_004107"
     CDS             complement(1698..2297)
                     /locus_tag="OO150_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5518043.1"
                     /translation="MPAGKSLTPDDVRPMLEQMVTEAVSHIPVPRDGRDYDPDVLQKA
                     VLDAVSALPAPQDGRDATALEILPAIDVQKSFPRGTYATHQGGLWRAYEKTHGMRGWE
                     CLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTV
                     TWGGSLWHCNSMTEDKPREAHSSAWTLAAKRGRDAGGGK"
     gene            complement(2237..2494)
                     /locus_tag="OO150_004108"
     CDS             complement(2237..2494)
                     /locus_tag="OO150_004108"
                     /inference="COORDINATES: protein motif:HMM:NF014428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5518044.1"
                     /translation="MVAQDVAVKAHVNDQFGNPVAHQPVTFSAEPSSQMIISQNTVST
                     NTQGVAEVTMTPERNGSYMVKASLPVKVSPRMMCVRCLNRW"
BASE COUNT          534 a          823 c          626 g          542 t
ORIGIN      
        1 gagcgtggtc acatcaatat cctgtttctg accggcggtg aactggactt ccttgatggt
       61 gcagtccagg cgcagatatt ccgccttatc catagtttca gcagtcgccg gggcagatga
      121 aatcatcacc tgcgtcagct gtgagcgttc atacaaagca gacattctgc ctcctgataa
      181 taaaaaaccc gcacgcggcg gggtatggtt ttgtagaaaa aaagaaaaag tcacaccgtg
      241 acctgaaact ccagggttgc acggtaacag cggttttccg gaatatagtc ctgcatttca
      301 ctgacggatc ccggggccag cagcattatg gcttcacggg cgtcctgacg tatctgacgc
      361 gcctgcgtca cagtcccggc ataaacgtct atctgcaccg acactgagga ctccgcctgc
      421 ccgcccatca cgtccgctga caccgatgaa atcaggctga aaaccaccca cggaagcgcc
      481 accgacggcc tgccatccag caggggggcc acatacgggg acacctgccc gccggcaaga
      541 tgcgccagat gaggatacaa atccgcctcc gtcatcgtct cagtacctca tcaatggccc
      601 ggttcatccg cgcaatcgcc acctgagctg cctgttcact gcgcacatca aacgccgggc
      661 gcacaaacgg gtgcggtggc atattcacgg tccccatttc cacaaaccgc cagtagaaag
      721 cattgcgcgg gttatccgcc ttcatggtgt tatcgctgtt accggtgtcc ggattaacac
      781 cacggatatg gacaccggat tccatcccgc catcgcggga gcgccgggaa aggatcacca
      841 cattgcggcg cagttttccc ctgcgtaccg gtgtccgtga caccacttct tctttcagca
      901 cattcgcacc cgcacgggtt gcctcacgca gcacccggtt attttccgca ccactcagaa
      961 gctgcaaatc gcggctgatg tcctccagcc ccgaaaaatc cagcaggatt tcgatcattt
     1021 ttcccctccc agccgacaga gaatttccag acgcccgccg gtcgcatccg gcacgggcag
     1081 cccgacaacg ttcaggatcc ggtcacgcca tggaccactc agcacatgaa gtcgtgacgc
     1141 tgccgtgatt tcccgaccgg actgaccgcg cacccagatg cggatttccg cctgcgccat
     1201 ttccgcaccg gactgcatcc gctcccggct gctcctgcct cggatatccg catgaatttt
     1261 cccgcatgac acccattctt ccgtcatttc tccggcagcg ttacgggtta acaccgggtt
     1321 cagaacactt atcatctatg tcagacgacc tgcagatatt gccattcccc ctcctcataa
     1381 caccgtcgga caacgcaaat cgtaaatcag cacggaaaca gaaaacggca gctccccctg
     1441 aagcagttct tcccgctccg caagatccgg attccggtac agcatcccgg tcagtcgcat
     1501 ggcagccccc ttcatccggg ttaatgcctc gcccgggatc agttcaccgt cctcacggag
     1561 cactttatcc cggctgccct gaatgtaggc cagcagcacg gcggtagcct gacgaacctt
     1621 gtccatcagc atgtcatcat ccgcgtcatg gtcgacacgc agatgtgcct tgatctcttc
     1681 cagtgtcagt aatgccgtca ttttccgcct cctgcatccc gcccacgttt tgcagccagg
     1741 gtccaggctg atgaatgagc ttctcggggt ttatcttcgg tcatactgtt gcagtgccac
     1801 agcgagcccc cccacgtcac cgtatcgccg gggtggtagg tttcaccggc tctgaacaca
     1861 ccgcggtaga gcatcaccgg cagggaaaat gttttttccg tacgctggcc actgctctgc
     1921 cggaccacca cagagaacaa ccgctcaccc gtcatgctga cgtcaatatc cgccaccccg
     1981 tcaaccaggc attcccatcc ccgcatcccg tgcgtttttt catacgcccg ccagagtccg
     2041 ccctggtgtg tggcatacgt gccccgggga aaggattttt gaacgtcaat ggcggggagt
     2101 atttccagag ccgtggcatc acgcccgtcc tgcggagccg gcagggcact caccgcatcc
     2161 agaaccgcct tctgcagaac atccggatcg tagtcacgac catcacgcgg aacagggata
     2221 tggcttacag cctccgtcac catctgttca agcatcggac gcacatcatc cggggtgaga
     2281 cttttaccgg cagggatgct ttcaccatat acgaaccgtt tctttcgggc gtcatggtga
     2341 cctcggctac accctgcgta ttagtagaga ccgtattctg gctgatgatc atttgcgagg
     2401 atggctctgc actgaatgtc acgggttgat gcgcaaccgg gttgccaaac tggtcgttaa
     2461 cgtgagcttt aactgctacg tcttgtgcga ccattacctg accttccggt atctccagac
     2521 tggta
//
LOCUS       DAJTNN010000289         2513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTNN010000289 DAJTNN010000000
VERSION     DAJTNN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.296"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..957
                     /gene="torZ"
                     /locus_tag="OO150_004109"
     CDS             <1..957
                     /gene="torZ"
                     /locus_tag="OO150_004109"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5518045.1"
                     /translation="WTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQ
                     FEARNDFDVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNA
                     FWQQNKLIEMRRSEKNEQYVRYADFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPA
                     HPTWLAPDEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDA
                     ARFGIANGDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVL
                     TADIPSSQLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
     gene            complement(1122..2093)
                     /gene="cmoB"
                     /locus_tag="OO150_004110"
     CDS             complement(1122..2093)
                     /gene="cmoB"
                     /locus_tag="OO150_004110"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5518046.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2090..>2513)
                     /gene="cmoA"
                     /locus_tag="OO150_004111"
     CDS             complement(2090..>2513)
                     /gene="cmoA"
                     /locus_tag="OO150_004111"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5518047.1"
                     /translation="PTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIY
                     QGLNPGGALVLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTD
                     SVETHKARLHKAGFEHSELWFQCFNFGSLVALKAEDAA"
BASE COUNT          649 a          630 c          645 g          589 t
ORIGIN      
        1 tggaccgcag ctgctaaaca tgcagatatc gtattaccga tcaccacatc gtttgagcgc
       61 aatgacttga cgatgaccgg tgattacagc aaccagcata ttgtgccgat gaagcaggct
      121 gtcgctccgc aatttgaagc gcgaaacgat tttgatgtgt ttgccgatct tgcggaatta
      181 ctgaaacctg gcggaaaaga gatctatacc gaaggtaaag atgaaatggc gtggctgaaa
      241 tttttctatg atgccgctca gaaaggtgcc cgtgcgcaac gcgtgactat gccgatgttt
      301 aatgccttct ggcagcaaaa taaactgatc gaaatgcgcc gcagcgagaa gaacgaacag
      361 tacgttcgtt atgctgattt ccgcgccgat ccggtgaaaa atgcgctggg tacgccaagc
      421 ggcaaaattg agatttactc caaaacgctg gaaaaatttg gctataagga ttgcccggca
      481 catccaacct ggcttgcgcc tgatgagtgg aagggtaccg ccgacgagaa gcagttgcag
      541 cttctgaccg cacatccggc acaccgttta catagtcagc ttaactatgc ggaactgcgt
      601 aaaaaatatg cggttgcaga tcgtgaacca atcactattc ataccgaaga tgctgctcgc
      661 tttggtattg cgaatggtga tctggtgcgt gtgtggaaca aacgtggtca gattctgaca
      721 ggcgcggtgg tgactgacgg gatcaaaaaa ggcgtggtat gcgtgcatga aggtgcatgg
      781 ccagatctgg aaaatggctt gtgtaaaaac ggcagtgcga acgtgttaac ggcggatatc
      841 cccagctcgc agctggcaaa tgcctgtgcc ggtaactctg cgctggtgta tatcgaaaaa
      901 tatacgggta acgcaccgaa gttaacggca tttgataagc ctgctgttca ggcgtaagaa
      961 gtgaatagcg cccggaaacg ggcactgagg ttatgcaaac gtaaaactgc ctgatgcgct
     1021 atgcttatca ggcctacata gtccctgcaa tagattgaat tagcaagggt ttgtaggccg
     1081 gataaggcat tcacgctgca tccggcagta ataccagacc tttacggctt gcgcgcaatc
     1141 agcaccgcgc gtttaggcgc aggataacct tcaactgtct tactcggatc atgtgggtcg
     1201 aggaaatcgg acagagattc ggtaaccatc cattcagtgc gtcgctgctc ttcagtagtg
     1261 gtaacgcaca catccacaac gcggatatct acaaaaccac acttcttcag ccagtttttc
     1321 agcgccagcg cggaagggat gaaatagaca ttacgcattt gcgcgtaacg atcgcctggt
     1381 accagcaccg tgttctcatc gccatcaata accagcgttt ccagcaccag ttcaccctca
     1441 ttcaccagtt gatctttcaa ctgccagaga tgctccagcg gcgaacggcg atggtaaagc
     1501 acgcccatcg aaaagacggt atcaaaggct ttcagtgccg gaagctgttc aatacctaac
     1561 ggtaacaaat gtgcgcgctg atcgttaccc agtagtttac gcaccgcttc aaactggcac
     1621 agaaacagtt gcgtagggtc gatccccacc gcgaggtgcg cgcctgcgcc aatcatgcgc
     1681 cacatgtgat aaccgctgcc gcagccgaca tccagaatgg tgcgtccggt taaatcagaa
     1741 agatggggca gaacacgatc ccatttccag tcggaacgcc attcggtatc gatgttgacg
     1801 ccatacagtg agaacggccc tttgcgccat ggcatcaggt tccgcataag cgtttcaatg
     1861 cgtttaattt gcccggtgct cagtggctct tcgctttcgg cggttacgct atgcaataaa
     1921 tccagacgat acggtttaat ttcaggcaga aattccaccg cgttggacca ctgcttaaac
     1981 agcccgtgct gctgctcgcg ctgccagtta gcaatctgcg cgggcagcgt ttcgagccag
     2041 tgtgaaagat gatttttggc aatcagagaa taaaagttac caaagtcgat catgcagcgt
     2101 cctctgcttt taatgccacc agtgaaccaa agttaaagca ctggaaccac agctcgctat
     2161 gctcaaaacc ggctttatgc aggcgtgctt tatgggtttc cacggaatcg gtcagcatca
     2221 cgttttccag catgctgcgc ttctggctga tctccagttc gctgtaaccg ttggcacgtt
     2281 taaagtcgtg gtgcatgttg aacaacagtt caccaacttt ggcatcttcg aaactgaatt
     2341 tttccgaaag caccagcgcg ccgcccgggt tcagtccttg ataaatttta tccagtaatg
     2401 cctggcgctc ggaaggttcc aggaattgca gggtaaaatt cagcaccacc atcgatgcgt
     2461 tttcaatggc gatatcgcga atatcacctt caatgacgtc tactggcgta ggg
//
LOCUS       DAJTNN010000290         2508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTNN010000290 DAJTNN010000000
VERSION     DAJTNN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.297"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..443)
                     /gene="curA"
                     /locus_tag="OO150_004112"
     CDS             complement(<1..443)
                     /gene="curA"
                     /locus_tag="OO150_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5518048.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVRLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQP"
     gene            624..1142
                     /gene="mddA"
                     /locus_tag="OO150_004113"
     CDS             624..1142
                     /gene="mddA"
                     /locus_tag="OO150_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5518049.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1139..1588
                     /locus_tag="OO150_004114"
     CDS             1139..1588
                     /locus_tag="OO150_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5518050.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1589..1822)
                     /locus_tag="OO150_004115"
     CDS             complement(1589..1822)
                     /locus_tag="OO150_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5518051.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1908..2081)
                     /gene="ortT"
                     /locus_tag="OO150_004116"
     CDS             complement(1908..2081)
                     /gene="ortT"
                     /locus_tag="OO150_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5518052.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2276..2371
                     /gene="yncL"
                     /locus_tag="OO150_004117"
     CDS             2276..2371
                     /gene="yncL"
                     /locus_tag="OO150_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5518053.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          604 a          615 c          612 g          677 t
ORIGIN      
        1 ttaggctgac cgatatccag cagccccata taagcggtaa agcctggcat ccccagcaca
       61 cccagcgacc acgatggatt ttgcggatga tcgccaagtc tcaccagatc atcaccactg
      121 gatatgtcat agtcctgcca tccactgtag cccagcaccc agtcgccagg ctgataatca
      181 ggatgattcg actccacaac atggctcacc gtgccgccga ccatcacgcc gccaatatca
      241 acaggcggtg aataagatgg ctcatcgctc atacgtccac gcatatacgg gtctagggac
      301 aaataaactg tgcgcagtaa cacctgtcct tcacccggtg tggcgacatc atcttcttca
      361 agacggaaat tctccggaac cggtgcgcca tgtggacgcg aggccagaac ccaacgtcga
      421 ttacgctgct tttgttgccc cataatgctc tcctgtgctt ttggcatgaa tccatcaata
      481 gtagcaggct cacgggattg gttttgtttg cagccgtcac atcccctctg caacacgcaa
      541 taatttcatc gaggaatccg ttgcttcggg ctgttttcga tagtattgat aacatcatca
      601 tcattatttt atcgaggcca gacatgtcca tccgttttgc ccgcaaagcc gattgtgctg
      661 ccattgcgga aatttataac cacgccgtgt tgtatacggc ggctatctgg aatgaccaaa
      721 cggttgatgc agataaccgc attgcctggt ttgaagcgcg gaccatagca ggttatccgg
      781 tgctggtaag tgaagaagat ggcgtagtga cgggatatgc ctcgtttggc gactggcgta
      841 gtttcgatgg ttttcgccat accgtggaac attcggttta tgtccatccc gatcatcagg
      901 gcaaaggtct ggggcgtaaa ttgttaagcc gattgattga tgaagcgcgg gatagcggga
      961 agcatgtcat ggtcgccggg atcgaatcgc aaaatcaggc ctcgctgcat ctgcaccatt
     1021 cgctgggatt tgtcgtcacc gcacaaatgc cgcaggtggg cactaaattt gggcgctggc
     1081 tggatctgac atttatgcag ttgcaactcg acgaacgcac tgaaccggac gcgattggat
     1141 gaatcagtcg ctcacccttg cttttttgat tgccgccgga attggtctgg tagtccagaa
     1201 cacgctgatg gtgcgcatca cccagacctc ctctaccatt ctcatcgcca tgttgctgaa
     1261 ctcactggtg gggattgtgc tctttgtttc tattttatgg tttaaacaag gcatggcggg
     1321 gtttggcgaa ctggtgtcca gcgtgcgctg gtggacactt atacccggct tgctgggatc
     1381 gttttttgtc ttcgccagta tcagcggata tcagaatgtg ggagcggcga caactattgc
     1441 agtactggtc gccagccagc taattggcgg tctgatgctg gatatcttcc gcagtcatgg
     1501 cgtaccacta cgggcgttgt tcggcccaat ttgtggcgca attttacttg tggttggcgc
     1561 ctggttggta gccagacgct cattttaatc agaggatggt gccgcctttg gttaactgtt
     1621 cgtggcgcgc ttccatatct tctttatgct tgcgaccgtg atgagcaata gcggtgcgca
     1681 aacgctgctg ctgggtgtag cgatcttccc gacttaactc cgcgtcatcg ctcagggcga
     1741 ttaataattc gttcatatgg gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga
     1801 tgacttcatc cagatgcgac atactttctc cttaaccact tgtgaaattt atcgattcat
     1861 ccaaatgata taagtctgcg tggtgccgtc aagccagtga gtaagattta aaacagtgaa
     1921 aacaacagcg ccaccgggaa actcatcggc catgttgccc ccaccagaaa ggcgcttaag
     1981 aaacggatgc gtttcttatc gtgagaaaga aaccaggtga taagaaatgc gattgctgcc
     2041 ataaccgcat aaaaaacaag catatgttga tagagagaca tgtacgggcg tccagccaga
     2101 tgaataaaac gcgcgcaaat atgcgtcatt ttttgacgtg catcaagttt tttcaccagt
     2161 ttgagaaaaa atcatcaaac agcaacttgc ccttatccat cttcagaatg agtattattg
     2221 cggattttct taacccgtac tatacacata ggggcaatga taaaaggtgg caaaaatgaa
     2281 tgtttccagt agaactgtag tactgataaa tgtctttgct gctgttggtt tgtttactct
     2341 tatctctatg agatttggct ggtttatttg atgtagtgat tttgcacgca gcacgtactg
     2401 tcagagaaat caactctctg acagtacgca agatcttctc atactctcca ggtttacaac
     2461 attatcctta atatttactt ctttttgctg gcatctacat tgaaatga
//
LOCUS       DAJTNN010000291         2451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTNN010000291 DAJTNN010000000
VERSION     DAJTNN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.298"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            545..1084
                     /locus_tag="OO150_004118"
     CDS             545..1084
                     /locus_tag="OO150_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5518054.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(1310..1633)
                     /locus_tag="OO150_004119"
     CDS             complement(1310..1633)
                     /locus_tag="OO150_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR5518055.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1917..2111
                     /locus_tag="OO150_004120"
     CDS             1917..2111
                     /locus_tag="OO150_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5518056.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
BASE COUNT          834 a          577 c          512 g          528 t
ORIGIN      
        1 ttatcgccac tggcagcagc cactgataac cgaattagag gagtaagact tgaagtcatg
       61 cgcctgttaa ggctaaactg aaagaacaag ttttggtgac tgcgctcctc caagccagtt
      121 acctcggctc aaagagttgg tatctctgag aaccttcgaa aaaccgcctt gcagggcggt
      181 tttttcgttt acaggcagta gattacgcgc agaaaaaaag gatctcaaga agaaccttta
      241 attttttttc acacttggta tcctacttta acccataaaa taaatactta caccgacacg
      301 cccttaaaca aaagaaaaaa aaacaataaa aaacaccgca aaaatggatt gttaacaaat
      361 gaaaatactc aattcgaatc tcataacaaa catatcatca acaataagaa atcaccttct
      421 aacatcatcc aaaaatataa aaacacaccc atcaccaaac atggacgtaa caagtcatcg
      481 tcgtttattc cagaaagacg gaataggatt gagccaaaca ggaaaagcaa cacaacttgt
      541 cattatgtcg catggtggat ggaaagagat ctcccatcca caaacactat tcacgcgtca
      601 aaaaggcgat ggatggactg tagtccccaa aaacctaaga attgattttt atacaaaaga
      661 caacgacttc acaaagggac taagcgttct ttcagaggta aacaaacgcc atactgaagc
      721 tcaaaaaggg ttaacaccat cactaaacat aagcaaagat gacctaaaag ttctagcgaa
      781 cgcgcgcaat atacctcaat caaaattaga agaagaaatg atgtcacaag ccatatatag
      841 aaaagaatac gcaacaagta atgagaagat aaaaaactat gctctatatt accatgaaca
      901 agcccaaaat attatacaaa gacatcaaga tggcttagga aacaaaaata ttgatatagc
      961 atttataact gataaaaatc ataagaagca cctttctgac atatttaaag taataaatga
     1021 atcaggagta aaatatgatg taattcactt tggcgcttgc cgagtaaata gagaggagaa
     1081 ataatcatca tttaccataa tcccttaatt gtacgcacca ctgaaacgcg ctgaacgcga
     1141 ttctgacgca gacaccacaa aaatagatgt tttaccccgg aaaaacagga aggatgcgtc
     1201 aggatcgttt tcagctcgct gcatagctat gcatgaaagc gaatggagat cactttggga
     1261 gcttaccgtg ttcataccgt ctgttttcaa cagttttctc cccgggaagc taatctgcca
     1321 taagcctgga taacagggca cggtgatact ccgtaatagc gatcagcacc tccgcatact
     1381 ccctgtcccc gatacgcttg ccgtcgataa agagtttttt ctgtaacgcc cccagtctca
     1441 taacttcttt cagtacccga tcatcagtca gtgagttaac gttcttaccg agagctgccg
     1501 cccgtaaaaa accggaaacg gtcttgccgc attcagcagc cctttttctg atttcctggt
     1561 cttcttctgc cgtcagacga acagggcgac tgatagcccg cctgcgcgta ttacttccac
     1621 ttcgttttgt cataaaacat ggctccgtat ctgacatggg tgtcggggca aagccctgac
     1681 cagggcaatt gtaatagcgt gcatgtatgc gcggtataac aattgcacat cctgtcctgt
     1741 cagcaagctc gaattaccga caaaggcacg gcagtttttt ggcagttaca gtgttgtctg
     1801 acagaaatgc ccgtcaaaaa gccaggcggc agaatagcgg cagcaaaaca gtcgaaaagt
     1861 ggtacgcgat ataatcacat aacgcagatg acagacagaa cggagaaaac gaaaagatga
     1921 acaagcagca gcaagccgta ctcaacatgg cgggattcat aaaaagccag agcctgacgc
     1981 tgctcgaaaa actggacgca ctcgatgctg acgagcaggc caccatgtgt gagaaactac
     2041 acgaactcgc agaagaactc cagaacagca tacagacacg ctttgaagta gaaaatagca
     2101 cagagagata acgctcagga caggatgtcg aacggcacaa cagagcgtaa ggagaaaatg
     2161 tcacataagg cgctcttccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
     2221 gcggcgagcg gtatcagctc actcaaaggc ggtaatgcgg ttatccacag aatcagggga
     2281 taacgccgga aagaacatgt gagcaaaaag ccgaaggcag ggacaaaggc cgcgcaggtg
     2341 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2401 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg a
//
LOCUS       DAJTNN010000292         2420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTNN010000292 DAJTNN010000000
VERSION     DAJTNN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.299"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(10..1383)
                     /locus_tag="OO150_004121"
     CDS             complement(10..1383)
                     /locus_tag="OO150_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5518057.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            1560..2351
                     /gene="kdgR"
                     /locus_tag="OO150_004122"
     CDS             1560..2351
                     /gene="kdgR"
                     /locus_tag="OO150_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5518058.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
BASE COUNT          703 a          567 c          639 g          511 t
ORIGIN      
        1 gcgctttttt tatgccctgg atcgtggctg agtgatacgt aaaccactga cacaggcagc
       61 aatcactgcc agaatagccg cagccatcag cgaaacgtgc gtaccattat caccgaactg
      121 atttagcatc agcgccacca gtgccgcgcc gctactctga cccagtagac gagccgttcc
      181 taacatgcca ctggctccac cgctacgttc gcgaggagcg gaggtaataa tggtgtggtt
      241 attgggtgac tggaacaagc caaatccagc gccacataag atcatcggcc agataatatt
      301 gatatccgca ggtgacgcgg gcagcagaac cagggaaaaa agccccgcag ccatgatgaa
      361 caaccctaga gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccaacgg
      421 agccatcacc atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact
      481 acgcccgagc acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca
      541 gaaagagcaa acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac
      601 cggtagcagc ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac
      661 cattaccacc agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa
      721 cgcagtgata agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg
      781 gggtttactg gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat
      841 aatacctaac ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc
      901 tgcagcaatt gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat
      961 ccctctcccc agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat
     1021 caacgctgca ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc
     1081 agaaagggcg cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg
     1141 tcgatagcca aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat
     1201 ttgataggcg ttcactaccc agatggaact ggctggcgtg gcatgaaggt ccgtggcgat
     1261 tgttggcagg gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac
     1321 aatggttaat atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg
     1381 cataggaatt ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt
     1441 cacggtggat gtcgcagaat tgtaactaac cggcaaaaga tggattgcga cccccctggt
     1501 gcgaaattat aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa
     1561 tggctaacgc agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg
     1621 gcattttgca ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg
     1681 tcatgatgtc aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg
     1741 tggcgcagga aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg
     1801 ctcgcgcgtt acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct
     1861 cccgcctgac caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca
     1921 ttcacaaaat tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc
     1981 cgctgtacag caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga
     2041 agcaaattct tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag
     2101 aagcgttatt acccgttctg gaccaggtgc gcgagcaggg gtatggcgaa gataacgaag
     2161 agcaggaaga agggctgcga tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca
     2221 ttgccggttt gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat
     2281 atgtcgcaat gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact
     2341 atccgttctg atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag
     2401 catgagaaaa ataaagttga
//
LOCUS       DAJTNN010000293         2368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTNN010000293 DAJTNN010000000
VERSION     DAJTNN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.300"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..809)
                     /locus_tag="OO150_004123"
     CDS             complement(<1..809)
                     /locus_tag="OO150_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR5518059.1"
                     /translation="MTIEKSRFSSGAAPQPNVNTGVINGNDFAAIVPVIAGRIGGREA
                     NIVSAKALHKALGVGRDFTNWIKGRIDQYGFIAGTDFIRVENLSTPKRASAKTRQQIE
                     HDYLLSLDMAKEVAMVERNEQGRAIRRYFIQCEEELQRSVPEIAARYRRQLKARLSAA
                     NNFKPMCDALNMARAEMGKTTQQRHYSNESNMIARIVLGGMTAKQWAQANGITGEPRD
                     SMNAAQLEHLAYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEVNHA"
     gene            complement(823..1254)
                     /locus_tag="OO150_004124"
     CDS             complement(823..1254)
                     /locus_tag="OO150_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518060.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYASELERRLDIQLERIDEKNPDGIGSHLRYRFTGR
                     GDVLKVIRLVNHNADAGGYQGLSQPEIADILNLYPDNLTAA"
     gene            complement(1254..1457)
                     /locus_tag="OO150_004125"
     CDS             complement(1254..1457)
                     /locus_tag="OO150_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR5518061.1"
                     /translation="MNIDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(1570..>2368)
                     /locus_tag="OO150_004126"
     CDS             complement(1570..>2368)
                     /locus_tag="OO150_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518062.1"
                     /translation="LLEIDIDDLLIMAAAESIYLSAYLCGYDGTLHSIASLYRNCSLF
                     PDVKEDCLLLADIITEVEDEKSFKAGVVNHIDCHVLGLWHVNASYILDIAGLDSIDTP
                     ARIFSPAHRKRELDVSANYYFFLDYTKHAFHEISVEDLYITRPWVEYIGECMDKNQPI
                     YSPETRINLQALYEDVKNVEHGNRERHRQKREICLLALIYVKRHYPEECKGKNGKETN
                     EAWANATINHWALLGGGYDEPSIDYLKKLIADADRTPEERTTAGKKR"
BASE COUNT          509 a          590 c          571 g          698 t
ORIGIN      
        1 tacgcatggt ttacctccag acgtttagcg agccagcgtt gcgacaggcg ggtgagttct
       61 cctttgcgct gctcatattc catgcccata tcaattaacg tgatattggt gctttcgaga
      121 taggcgaggt gttcaagctg tgctgcgttc atgctgtccc gtggttcgcc agtaatgcca
      181 tttgcctgcg cccactgctt cgccgtcatg ccacccagca cgatacgggc gatcatgttg
      241 ctctcgttgc tgtagtggcg ctgctgcgtc gttttgccca tctcggcacg ggccatattc
      301 agcgcatcgc acattggctt aaagttgttt gcggcactga gacgggcttt tagctggcga
      361 cgatagcggg cggcgatttc aggcacgcta cgctgtaatt cttcctcgca ctggatgaaa
      421 taacggcgga tagcgcggcc ctgctcgttg cgttcaacca ttgccacttc tttagccata
      481 tccagcgata gcaggtaatc atgctctatt tgctggcgag tttttgcgct cgcccgtttt
      541 ggcgtgctca aattttcaac acggataaaa tcggttccgg caataaaacc gtactggtca
      601 attcggcctt taatccagtt agtaaagtcg cggcccaccc ccagcgcttt atgcaacgcc
      661 ttagcgctca caatattggc ttcacgcccg ccaatgcgtc cggcaataac gggaacaata
      721 gcggcaaagt cgttgccgtt aattactccc gtgttaacgt tgggttgcgg ggcggccccc
      781 gaactgaatc ggcttttttc gatagtcata ttttcggctc cgttatgcgg cggtgagatt
      841 gtccgggtat agattgagaa tgtcggcaat ttcaggctga gaaagtccct gatagccgcc
      901 agcgtcggcg ttatggttca caagccgaat aacttttaga acgtcaccgc gcccggtaaa
      961 gcgatagcgt aaatgtgacc ctatcccgtc ggggttcttt tcgtctatac gctcaagctg
     1021 aatatcaagc ctgcgctcta actcgctggc gtagttcctt ccagacgaaa ggcggcaata
     1081 acgcagaatg tcattttctg tccatccctc tataccagta cgcagcatat agactctggc
     1141 acgatgcttt ttcggtgtgc gctttggtgc ttgaagggat tttgtaaggg gcgtaacatc
     1201 agatacgcga atatctgaat tagccgcctg taatgggcgg tttttctttt ccatcaggcc
     1261 accttcccgc ggcgttctgc cagccagttg tgaatctcaa ccgcatcaaa cgcagttatt
     1321 ttttctccca gctttaccgg acgtggtagt gaaccgtttc ttactttacg gtcgatggtg
     1381 gatacactaa cgcccagcaa ttcagccagg cggaagcgtc tgatatagcc ttttgtaggg
     1441 atagtgtcga tgttcatgat tcccgtaacc tcttagcgat agttgttgct tcgttacggg
     1501 tatttaatgg cgtgcacgct caaaaaggaa tgatcgtatt ttttactatc gtgatagtaa
     1561 aatttactat cacctttttt tccctgccgt ggtgcgttct tccggtgttc tgtctgcgtc
     1621 ggctatgagt ttcttcaggt aatctatgga tggctcatca tagccgcccc ccaaaagcgc
     1681 ccagtggttt attgtggcgt tcgcccatgc ttcgttggtt tccttcccgt tcttcccttt
     1741 acactcttct gggtagtgcc gcttcacata aatcaacgcc aagaggcaaa tttcgcgctt
     1801 ttgcctgtgg cgctctctgt ttccgtgctc aacgttttta acatcctcat aaagagcttg
     1861 taagtttatg cgagtttcgg ggctgtatat gggctggttt ttgtccatgc attcgccgat
     1921 atattcaacc cacggccttg ttatgtacaa atcctctacg gatatttcgt ggaaggcatg
     1981 ttttgtataa tcgagaaaaa aataataatt cgcacttacg tctagttctc tttttctgtg
     2041 tgcggggctg aatatgcgtg cgggagtgtc aatagaatca agccctgcga tgtcgagtat
     2101 ataggaggcg ttaacatgcc atagcccaag cacatggcaa tctatatgat ttacaacgcc
     2161 tgccttaaag cttttttcgt cctctacttc agtgattatg tcggcaagta ataagcagtc
     2221 ttctttgacg tctgggaata aagagcaatt gcggtaaaga ctcgcgatgc tatgtaatgt
     2281 gccgtcgtag ccgcaaaggt acgcgctcaa atatatagat tctgccgccg ccataatcaa
     2341 aagatcatca atatctattt ctaataat
//
LOCUS       DAJTNN010000294         2368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTNN010000294 DAJTNN010000000
VERSION     DAJTNN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.301"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..305)
                     /locus_tag="OO150_004127"
     CDS             complement(<1..305)
                     /locus_tag="OO150_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5518063.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYA"
     gene            complement(315..1697)
                     /locus_tag="OO150_004128"
     CDS             complement(315..1697)
                     /locus_tag="OO150_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR5518064.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1990..2084
                     /locus_tag="OO150_004129"
     tRNA            1990..2084
                     /locus_tag="OO150_004129"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2024..2026,aa:Sec,seq:tca)
     gene            2163..2327
                     /locus_tag="OO150_004130"
                     /pseudo
     CDS             2163..2327
                     /locus_tag="OO150_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280578.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
BASE COUNT          637 a          595 c          557 g          579 t
ORIGIN      
        1 tcagcataac gttctgtgtt ttcgattgtg accttcacgt cctgcaaaat attgagcgga
       61 taatgaggac cgttattcag cgctccctga aaatgctcaa tccgcacacc gacaatacct
      121 tcctgtgggg agaaaaagcg cagcgtaaat aaaggcgtat caagctgcca ggtacgttca
      181 cgcacatcac ggggggcagc atagaccacc atttcattat cctgctgttc aacctcgaac
      241 acctgaagcg ggtgaatcaa attgaggcca ggttgaatca accagtttcc atcgctaatt
      301 ttcattgccg ttccttagtt ctgcacttct tgagaggtga attgttgctg gcaacggcgt
      361 ttaccctgag ccagctgttc cataacggtt ttcagattgt gcgtcgtgag tgagtagtag
      421 cgtttagcga taatcgcgct cagcaaataa cagatcgccg gaacaatcgt gaatagcgca
      481 ataatgatgc taatcgtggc gctgttctgc gctttttctg ccgcatcata tccgccataa
      541 gccagcatcc agccgataag agcgccgccg aaggccaacc ccagtttgag cacaaacagc
      601 gtgccagcaa aactgatccc ggtcaggcgt ttaccattgc accactcgcc gtagtcgacg
      661 gtatcggaca tcattaccca ctggataggt gtcaccagtt gatgcaacac accaatcacg
      721 aagatgaaga caaacatagt gatgctggcc tgcatgggaa caaagaacat cgcgaggcta
      781 atcactgcca gcagggcgtt cgtccaccag aagatagtga ctttacattt ccagtcggtc
      841 agaggttttg ccagtgcgga accaatcagg ttacccacgc aataagtggt gagaaaagcg
      901 acaaacactt ccggcgtgcc caaaatccat gtgacgtaat acatcatcgc cccaccgagt
      961 acgcacaccg ccaggatatt gaaaatggtt agtaaaccga caatccgcca ctggtcgttt
     1021 tgccagatat cacgtaaatc ttcccgcata gacgttgttg taggtggtgc ttcaacgcgt
     1081 tctttagtgg tgaagaaaca aaatgccagc atcatgaatg ccaccacgga aaggaccgcg
     1141 ataccgccct ggaaaccgag tggtttatta tcaccgccaa ttaaattaac cagtggcatc
     1201 atcagaacag tagaaagcat gcctcccgcg gtcgccagca caaaacgcca ggattgcagc
     1261 gagatacgct gagtcgggtc attggtgatt acaccaccca atgcgcagta agggatattg
     1321 acgacggtat aaagtaaggt aagtagggtg taagtaattg ctgcatagat cattttgccg
     1381 ttcatactga gatctggcgt gctataggcc agtacacaga cgatcccgaa tggcagtgcg
     1441 ccaaacagta cccacggacg aaatttaccc cagcgagagc gcgttcgatc ggccaacaac
     1501 cccatgcaag gatcggaaat cgcatccagt gcacgagcga ccaaaaacat ggttccgaca
     1561 aaaccggcag gaatgccaaa aatatcggta taaaagaaca tcatatataa cattacgtta
     1621 tcgaaaataa tgtggctggc ggcgtctccc atgccataac caattttctc tttaacggac
     1681 aacacttcac tcttcatctg cttttccttg atgctatagg ggtgctgaga ccgataacgc
     1741 attttgtaaa ccatccctgt gagagcgaat attccgatat ctggttatta atttatgatt
     1801 cttgttttat gtgatcgtgg tagcgttaat tccgctcata tgtcattgta aaatatgggt
     1861 tttatatgaa ctataatgct ttcgtgataa tacgctgcgt gtattaggcg gaaaaaactg
     1921 atctggggga tgtagaaact caaggaagta gctataatgc gccccgcctc catgtagcaa
     1981 tcgaggcgcg gaagatcgtc gtctccggtg aggcggctgg acttcaaatc cagttggggc
     2041 cgccagcggt cccggacagg ttcgactcct gtgatcttcc gccaaaatgc ctcttctgac
     2101 gttctccaac cccttgtaat actgtcaatc cagatatttt ctactgctca atattcaatc
     2161 ggcttcagga atacctggtc taaggttttg tgatggacga tgagcggctg aagaatccgc
     2221 ctgtcggttc ttctgtcctc gaacgcatcc gcgatatgcg cgccagcatg tgccgtgttt
     2281 atttgcaggt gatttgcccc ctgctggctc tcagccaact gtatcgccat taaggtgaag
     2341 atgttgtttt acacgctcca taacaccc
//
LOCUS       DAJTNN010000295         2314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTNN010000295 DAJTNN010000000
VERSION     DAJTNN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.302"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(44..380)
                     /locus_tag="OO150_004131"
                     /pseudo
     CDS             complement(44..380)
                     /locus_tag="OO150_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(913..1239)
                     /locus_tag="OO150_004132"
     CDS             complement(913..1239)
                     /locus_tag="OO150_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518065.1"
                     /translation="MFKYAEMLMAEEDNKVCELIFKQQDNLILKINDRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLILCALGVNSWRGKSKYLDYYLLRDEMPVRLVSIIFPTLL
                     ALFLLA"
BASE COUNT          760 a          446 c          403 g          705 t
ORIGIN      
        1 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
       61 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
      121 acgaccacag tattctggct cctgtgtggt catgctttaa ttctgcgttc cggacagata
      181 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
      241 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
      301 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
      361 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
      421 ccatacagga taattagagg tcagtttgat tattcacaat tcattatcag cattttctgt
      481 ttctgacgaa atcaacatga aaagaaccat atatgataat tattataata acggctttaa
      541 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
      601 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
      661 attgataaaa aaacgccagc aacacatagt gccggcggag ggaatacccc atggagaaaa
      721 tgtgatgcct agagcaccat gaatatataa ttaaaaatag ttagagttgt caatactttc
      781 acaaaaataa ttttcgtagt ttcgtagtat agaaagatat ttttatgcat gacctacctg
      841 aatttgctcc gggtagaggt tataaataaa aattgaatca cgacaaacac aatattcata
      901 gtatggcgat gcctacgcca gcaagaatag cgccaataat gttgggaata taatagacac
      961 cagacgcaca ggcatctcat ctcttaacaa ataataatca aggtatttac ttttgcctcg
     1021 ccagctgttt acgcctaaag cacacaagat taaccattca ataacaaatg gcaatatcca
     1081 tagccatacg actttacctt gtaatgttcg gtatttcttt ataattattc tgggaaatct
     1141 atcatttatt tttaaaatca aattatcttg ttgtttaaaa ataagttcac atactttatt
     1201 atcttcttcc gccatcaaca tctctgcata cttaaacatt tcagaacgtt cctttagcac
     1261 agaagagtaa ttatatgtcc agttcccagg caataatgct tatggatatt taactaataa
     1321 tttagagaat attttgcaat aatatttggc tgtattcaga aatacctgaa aaatcatact
     1381 atacagcccc agggaatgga taaagattct aacaaagcat ttcaacaata tatgcttgtt
     1441 aaaaatccat atcgaaatgc cgttgcagca ttaatatatg cctcattcat aaaatgtaaa
     1501 agagcaagct cgtaccagta ggggggaaat tcattgacat gtcctgtcaa tacgtacaga
     1561 gccctgtcat gcttgccagc acgtaacaat tccgtcccca gtaaccagcg tgtcccctga
     1621 ttattctcag ggttatatgt tagtatttta tcaatgagta taacggcatc ctgatgacgc
     1681 tgtaaatgaa cattcgccag aattgcagca taaagagcgc aaatgcatat gccagatgag
     1741 cgtgaatatc aatgatgtca ggagcatgtc aggcaagacg cctgagtttg ttgacgtaat
     1801 ttgtttcgtt tatttcatca ctatcgtatg cgtcaagagt tttttcaaac tcatcccgaa
     1861 atgggccaag cctggtttca ggaaataaga aataccccta gttattgttc acttaaaagt
     1921 ataatttgta attcaaatcc tgagcctgta aaacggggag taacatattc tattctgaag
     1981 agaataaaag tcgtgatgcg atgtatcaag cccggattgt aatcccagat taacatagat
     2041 cacaaaacaa cttattcttc actaacgtga agataaatat cgttgtatgc cttatcacga
     2101 ctacgaatac cagcaagata caactgacat acggaaagat accatgtttt ttactcccga
     2161 aaataacgct aaaaagctac ttccccatcg tttgtcctta gtattgtcag cgccaacaat
     2221 gtgggctgac atgataaagc tgtctaggaa aacgttcgcc tcctcagcgg acaatccaaa
     2281 tggtgattgt ccctattaaa cgtttatttt gaag
//
LOCUS       DAJTNN010000296         2313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTNN010000296 DAJTNN010000000
VERSION     DAJTNN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.303"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            368..2020
                     /locus_tag="OO150_004133"
     CDS             368..2020
                     /locus_tag="OO150_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5518066.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFTR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2007..2207)
                     /locus_tag="OO150_004134"
     CDS             complement(2007..2207)
                     /locus_tag="OO150_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5518067.1"
                     /translation="MTKKLAALQHQLSRKVKFSNNRQKQKHKIQQLHSHIANIRRDYF
                     HKVTATVSKKHVMIVNYVITAN"
BASE COUNT          719 a          431 c          541 g          622 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtcggtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 cacccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 ggggcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataacaaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtga gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg gaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gcctgttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccgtat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac aga
//
LOCUS       DAJTNN010000297         2290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTNN010000297 DAJTNN010000000
VERSION     DAJTNN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.304"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO150_004135"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO150_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5518068.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO150_004136"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO150_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5518069.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO150_004137"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO150_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5518070.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          612 a          487 c          540 g          651 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agcaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaggg caacagatcg cgtacccagt
     1021 ttgccggcca gtcttggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattca ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatacgataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aaccacggca gcctggcggc
     2161 tgcaaatcgg ggtgtggcag aatatgagct ttcagagtaa ccatccagaa tgagttccca
     2221 tgtcacacaa cctggagcac cagaaagtcc acaccagaat ggtgaaagag gttctgaaag
     2281 ccgtcgccag
//
LOCUS       DAJTNN010000298         2282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTNN010000298 DAJTNN010000000
VERSION     DAJTNN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.307"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..359
                     /locus_tag="OO150_004138"
     CDS             <1..359
                     /locus_tag="OO150_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518071.1"
                     /translation="CPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDRMPD
                     SPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASVRRI
                     VNMVALAVVEAQTQPL"
     gene            651..986
                     /gene="eutS"
                     /locus_tag="OO150_004139"
     CDS             651..986
                     /gene="eutS"
                     /locus_tag="OO150_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5518072.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            999..1478
                     /gene="eutP"
                     /locus_tag="OO150_004140"
     CDS             999..1478
                     /gene="eutP"
                     /locus_tag="OO150_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5518073.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            1453..2154
                     /gene="eutQ"
                     /locus_tag="OO150_004141"
     CDS             1453..2154
                     /gene="eutQ"
                     /locus_tag="OO150_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR5518074.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGVLHVRHEGQTMIAKAGDVMFIPKGSSIEFGMTSS
                     VKFLYVAWPANWQSL"
     gene            2151..>2282
                     /locus_tag="OO150_004142"
     CDS             2151..>2282
                     /locus_tag="OO150_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="catalyzes the conversion of cob(I)yrinic acid
                     a,c-diamide to adenosylcob(III)yrinic acid a,c-diamide;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HCR5518075.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     "
BASE COUNT          579 a          560 c          623 g          520 t
ORIGIN      
        1 actgcccgtc gtcgagcaaa atgcgtcagg cgctggaact ggtcagggat cgtgcgccag
       61 atctgatgat tgatggtgaa atgcacggcg atgcagcgct ggtggaagcg attcgcaacg
      121 accgtatgcc ggacagccct ttgaaaggtt ctgccaatat tctggtgatg ccgaacatgg
      181 aagctgcccg cattagttac aacttactgc gcgtttccag ctcggaaggt gtgactgtcg
      241 gtccggtgct gatgggcgtg gcgaaaccgg ttcacgtgtt aacgccgatc gcatcggtgc
      301 gtcgtatcgt caacatggtg gcgctggccg tggttgaagc gcaaacccaa ccgctgtaat
      361 tcttttttaa ctctcacgct taccctgaat attcagggta agcggtttgg ctgcaatata
      421 ttagtaaagc ttattactga gtttgcgaat aataaaaaag cagtctatat aatatctcga
      481 tattatttta tttatattca tgcgttgcat atgaaagttt atgcaccaca gcgaatatct
      541 ctccattctt agtgatctac ctcacctttt aaacgcgctt gccgaatttt gttatttact
      601 ctgacgaaaa attgtcacga tacacgaaag tttttcacag gcggcgactc atggataaag
      661 aacgcatcat tcaggaattt gtgcccggca aacaggtcac actggcgcat ctcatcgcgc
      721 atcccggtga ggaactggcg aagaaaatcg gcgttccgga tgcgggcgca attggcatta
      781 tgacgctaac tcccggcgaa acggcgatga tcgccggtga tttagcgctg aaagccgctg
      841 atgtgcatat cggttttctc gaccgcttca gcggtgcatt ggtgatttat ggctcggtgg
      901 gcgcagtaga agaagcgtta tcacaaaccg tcagcggttt aggtcgctta ttaaattata
      961 cgctttgtga aatgaccaaa agttaatacg aggcattcat gaaacgaatt gcgtttgtgg
     1021 gtagtgtcgg ggcaggaaaa acaacgttat ttaatgcgtt gcaggggaat tataccctcg
     1081 ccagaaaaac acaggccgtg gaatttaatg ataagggcga tattgatacg ccgggtgaat
     1141 attttagcca tccccgctgg tatcacgcct taattaccac gctacaggat gtagacatgc
     1201 tgatctatgt ccatggcgca aatgacccgg aaagtcggtt gcctgccggg ttgctggata
     1261 ttggcgtcag caaacggcaa atcgccgtca tcagtaaaac ggacatgcca gatgctgacg
     1321 tcgccgcaac gcgaaagtta ttgcttgaga cgggtttcga agagccgatt ttcgagctaa
     1381 acagccacga cccgcaaagc gtgcagcaac tggtggatta tctggcatct ctcaccaaac
     1441 aggaggaagc aggtgaaaaa actcatcaca gcgaatgata ttcgtgaagc acacgcgcgc
     1501 ggcgaacagg caatgtccgt ggttctgcgc gccagcatta ttaccccgga agcccgtgaa
     1561 gtggcggatc tactgggttt taccatcacc gaatgtgatg aatcgatccc ggtaacggcg
     1621 tccgtgcccg ccagcgtgcc agccgacaaa accgaaagcc agcgcatccg cgaaaccatc
     1681 attgcccagc tgccggaagg ccagtttacc gaaagcctgg tcgcacagct gatggaaaaa
     1741 gtgatgaagg aaaaacagtc gctggagcag ggcgcaatgc agccgagctt caaatcggtg
     1801 accggcaaag gcggcatcaa agtgattgat ggcagcagcg tcaagtttgg tcgtttcgac
     1861 ggcgcagagc cgcattgcgt gggcttgact gatcttgtga ccggagacga cggcagcagc
     1921 atggccgccg gttttatgca gtgggagaac gcattcttcc cgtggaccct gaactacgac
     1981 gaaatcgaca tggtgctgga aggggtgctg catgttcgcc acgaaggcca aaccatgatc
     2041 gccaaagcag gcgacgtgat gtttatcccg aaaggctcca gcatcgaatt tggtatgaca
     2101 tccagcgtga aattcctgta tgtcgcctgg ccggctaact ggcaatccct atgaaagatt
     2161 tcatcaccga agcatggcta agagcgaacc atacgctcag cgaaggcgca gagatccatc
     2221 tccctgcgga cagtcgcctg acgccctctg cccgggagtt gctggaaagc cgccatctgc
     2281 gc
//
LOCUS       DAJTNN010000299         2264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTNN010000299 DAJTNN010000000
VERSION     DAJTNN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.308"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            376..519
                     /locus_tag="OO150_004143"
                     /pseudo
     CDS             376..519
                     /locus_tag="OO150_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            666..934
                     /locus_tag="OO150_004144"
                     /pseudo
     CDS             666..934
                     /locus_tag="OO150_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1327..>2264
                     /locus_tag="OO150_004145"
     CDS             1327..>2264
                     /locus_tag="OO150_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5518076.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLAMANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEHSLADAVTAWFPENKQSDVSQIWHAFEHEE"
BASE COUNT          754 a          484 c          386 g          640 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcaaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacgtaaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaatttttta ccgaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttcccaa aaaacaagaa aatatcatcc
      721 ctgaagtatc aaatactata ttagtatctt ctactgttac tacagatgga tgtactgttt
      781 ttaataaaca aaaacagcca tatacgaatt gcaaatacct gctaccaacc gaacgaaaaa
      841 gttgaaattc acagctacag aaatacaatg gttgacaaag ataaatgaag ccggaattga
      901 tgaaaaacaa agccaacggt attctgattt ctgaagttaa tttcagactc gtttaagctc
      961 cattttcaaa aaaaaatata tcagggtttg ttactaataa cttctgaaaa cagaaaaaaa
     1021 atagagcagt aattctattt cagagaggag taactccatc tatcctcgat acgtactgaa
     1081 cgttatcgtg atatattcat catgctttct tcaccattat tcgagtacag ggagagacgc
     1141 aatgacctta aatacctttt tatggctgaa ttaccattct caggaataac agcagactcc
     1201 ttcccataga cggcttaggg ggaaagcaat acccggagaa agggtacaaa gcaaactgtt
     1261 ttaaaattaa taagctatat tctatttcac tagaattctt atcgctgata agaaatttta
     1321 gttataatga ttaaatcaac caatatacag gcaatcggtt ctggtattat gcatcaaata
     1381 aacaatatat actcgttaac tccatttcct ttacctatgg aactgactcc atcttgtaat
     1441 gaattttatt taaaagcctg gagtgaatgg gaaaagaacg gtaccccagg cgagcaacgc
     1501 aatatcgcct tcaataggct gaaaatatgt ttacaaaatc aagaggcaga attaaattta
     1561 tctgagttag atttaaaaac attaccagat ttaccgcctc agataacaac actggaaata
     1621 agaaaaaacc tattaacaca tctccctgat ttaccaccaa tgcttaaggt aatacatgct
     1681 caatttaatc aactggaaag cttacctgcc ttacccgaga cgttagaaga gcttaatgcg
     1741 ggtgataaca agataaaaga attaccattt cttcctgaaa atctaactca tttacgggtt
     1801 cataataacc gattgcatat tctgccacta ttgccaccgg aactaaaatt actggtagtt
     1861 tctggaaaca gattagacag cattcccccc tttccagata agcttgaagg gctggctatg
     1921 gctaataatt ttatagaaca actaccggaa ttacctttta gtatgaacag ggctgtgcta
     1981 atgaataata atctgacaac acttccggaa agtgtcctga gattagctca gaatgccttc
     2041 gtaaatgttg caggtaatcc actgtctggc cataccatgc gtacactaca acaaataacc
     2101 accggaccag attattctgg tcctcgaata tttttctcta tgggaaattc tgccacaatt
     2161 tccgctccag aacactccct ggctgatgcc gtgacagcat ggttcccgga aaacaaacaa
     2221 tctgatgtat cacagatatg gcatgctttt gaacatgaag agca
//
LOCUS       DAJTNN010000300         2221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTNN010000300 DAJTNN010000000
VERSION     DAJTNN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.310"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            72..393
                     /locus_tag="OO150_004146"
                     /pseudo
     CDS             72..393
                     /locus_tag="OO150_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(427..633)
                     /locus_tag="OO150_004147"
     CDS             complement(427..633)
                     /locus_tag="OO150_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518077.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1131..1204)
                     /locus_tag="OO150_004148"
     tRNA            complement(1131..1204)
                     /locus_tag="OO150_004148"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1170..1172),aa:Gly,seq:ccc)
     gene            complement(1283..2038)
                     /locus_tag="OO150_004149"
     CDS             complement(1283..2038)
                     /locus_tag="OO150_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5518078.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKTGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
BASE COUNT          589 a          522 c          486 g          624 t
ORIGIN      
        1 tgctaagtta atgcattaat ctaaaaacgc tttaaatcaa tacataacca ttatctcttg
       61 ggattattta catgcgcaaa agtttaatgc tgttacttgc ttcaacattg attatttctg
      121 gatgtgcaac aaaaaaacaa ctcgttccaa ctggtggaag caaatcagac ggcacagtta
      181 ggatgagtta ttcttacggt atgtttgaaa gtcctgttgt agatccacaa caaggcatga
      241 atgcagccaa agcgcgttgt gccgcttagg ggatattcag gagccgagcc ttttggtgga
      301 ttcacttctc aatgttcaca accttcttca tctggttgta tgcaaacact tgtgactatc
      361 gaatatcaat gcactggcga tattaagaag taataattag gggcttccag ccccttctat
      421 aaccattcat ggaacaggga caaccgaaat cactttaaac cctgaaacag ccgttactcc
      481 attagatgga gaattaaatc tcacagtatc ttttcttact gcatcatgaa tttcatcttt
      541 tgttggttca tgctctgaat ggaataaaac ctcttccatt ctaatttgat caccgtctct
      601 atgctcataa gtaatcttgt agctaacagt catatcaacc tcatgcaggg gaattgtgag
      661 attattatag acaatagaaa ccataaagcc ccgttggtga tacagcaaca agcgggagcg
      721 tagcgacttc atcagattgt cagaaagatc acaccttatc aggataaccg tgtacccgtt
      781 gggagcgtgg ctgaaacgct gatagagctt gcatatggtt tgcaaggacc aatatcacca
      841 taaacctgtt tacgcagcca caaggccgca catcgggctt aaatgcgtgt gttcagtctg
      901 tcgatgacat tacaatgcca ggatgggctt tatcttcacg ttgtgctatc gcggtgtgag
      961 aggtgcaact gccgccaacg ttaggttata ctgtcatctc accgtgtaac ttaacgtgac
     1021 agttactaag ggcggatcac aatggtagaa ctcctaagca aattaaatag gtaactgttt
     1081 aagttgctga cagagaagac ttttttagtc ttctccacct ggggagattc tggagcgggc
     1141 gaagggaatc gaaccctcgt atagagcttg ggaagctctc gttctaccat tgaactacgc
     1201 ccgcttcgag atgcgtaagg cattataaac cttacgctct ccttagcaag tgccgcgctg
     1261 ctgactgctg attaattcgc catcagcatt ttggcttgct gccctgcggc ggcaagtaac
     1321 gtagcggatc aattgccgtt gcacggtaac gaatctggaa atgcaggcga acagatgccg
     1381 catccgtgct ccccatagtg gcgatttttt gcccagtctt cacgctttgc ccattattta
     1441 ccagcatcgt gtcattatgg gcgtaagccg taatgtaatc ttcactgtgt ttaatcatga
     1501 tgagattacc gtagccacgc agctggttgc ccacatacac cacctttcct gcacccgcgg
     1561 cgtaaatagg tgtaccccgt ggagctgaga tatcaatccc tttattgccg ccatctgctg
     1621 tcgaatacgg catgataact ttccctgtcg ttggccataa ccaacaacgt tgccctactg
     1681 gcggccagga agatttcggt accgctgatg acggtgtaac cgatgcggtt ttggtcgttg
     1741 atttggcggt tgatttacgt gtactactgc tacttttcgc cccacccagt tttagtttct
     1801 gaccaacttc aatggtgtaa gggggggaaa tgccgttcag tcgcgccagt tcttttacgc
     1861 tggttcccgt ggtgcgcgaa atacgatata gcgtatcccc ccgtttcacg gtgtaaacgg
     1921 agccggaata tgttcctgta tcggatgatt tgctacccga acagcccgcc aaaagcagtc
     1981 caaccgataa caacatcacg atacccagag attttttatt caggcgtccc gcactcaaaa
     2041 tctttcctcg cttaaaaaac aagacgcctt atcatagcag ccagcctctt gatacccaat
     2101 ctcttgcttc tggaaaaacg tggtaataga gaaagaggtt aatttcattt ggcaaaggga
     2161 agtatcatca gcaaaatatt cgctgtagag gtatcgaact tcctgacttt ttaccctatt
     2221 t
//
LOCUS       DAJTNN010000301         2215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTNN010000301 DAJTNN010000000
VERSION     DAJTNN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.311"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(454..825)
                     /locus_tag="OO150_004150"
     CDS             complement(454..825)
                     /locus_tag="OO150_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518079.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(886..1383)
                     /locus_tag="OO150_004151"
     CDS             complement(886..1383)
                     /locus_tag="OO150_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518080.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1459..>2215)
                     /locus_tag="OO150_004152"
     CDS             complement(1459..>2215)
                     /locus_tag="OO150_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HCR5518081.1"
                     /translation="QLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
                     VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
                     RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
                     LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
                     AYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          526 a          584 c          537 g          568 t
ORIGIN      
        1 aacttgtcac aaagtgcata ggcaagagct ttggcggcag acacgtgcgt ttcctcttaa
       61 acataggtcg aacggatacc cgaggcaaag cggagcatta gtcccaaagc atctccggta
      121 gatccggagg ctccatactg aaggcatggc atcttgggca ttgatatttg aaacttctct
      181 ctagatagca gggatttctt ggccttgcct catgctgata gcttgtaaat atcacacgat
      241 ccttagcccc acaaagtgag catactccgt agctttcatt tatagaaatc gtcgttagat
      301 tgtcgcagca tgtgcacgtt ccgactattt gctcaggaag catcgccata ccttggccta
      361 ggtaaagaag atcagactcg tagccacatc ggtggcaact gagcttgtac gcaatgccca
      421 tctggttgtc tcacctgaat cgtaacgtca gagctattca tccatccatt cggcatcaat
      481 gtatgccact aagccgacca gctgaccgtt tggactgaag tatgttgcct gcaccccact
      541 tgacatctca tcaataccac cgcactgcgc taggcgccgg tcccacttgc ccacagaagc
      601 gtcaggatcg gctgagttcc ccaagaccct tgtgagccat gcctccttta actcaacctc
      661 tcgaatgatt gcgtcggcca gctcctccgc gttatctagt tcgacatagt ctggaattcg
      721 gacgtacgtt ctgaacacag ctggatactt acttgggcga ttgtcataca tgacatcaac
      781 aatgtacccg tttgtgtaac cgtctcttgg aggttcgtat gacactagtg gttcccctca
      841 gcttgcgact agatgttgag gcctaacatt ttattagaga gcaggctagt tgcttagata
      901 catgatcttc aggccgttat ctgtcagggc aagcgaaaat tggccattta tgacgaccaa
      961 tgccccgcag aagctcccat ctttgccgcc atagacgccg cgcccccctt ttggggtgta
     1021 gaacatcctt ttgccagatg tggaaaagaa gttcgttgtc ccattgttgg caatgacgta
     1081 gtagccggcg aaagtgcgag acccatttgc gctatatata agcctacgat ttccgttgcg
     1141 actattgtcg taattggatg aactattatc gtagttgctc tcagagttgt cgtaatttga
     1201 tggactattg tcgtaattgc ttatggagtt gtcgtagttg cttggagaaa tgtcgtagtt
     1261 ggatggggag tagtcatagg gaagacgagc ttcatccact aaaacaattg gcaggtcagc
     1321 aagtgcctgc cccgatgcca tcgcaagtac gaggcttaga accaccttca acagatcgcg
     1381 catagtcttc cccagctctc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
     1441 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
     1501 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttgtc caagataagc
     1561 ctgcctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
     1621 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
     1681 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
     1741 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
     1801 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
     1861 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
     1921 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
     1981 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag
     2041 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
     2101 atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
     2161 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttga
//
LOCUS       DAJTNN010000302         2210 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTNN010000302 DAJTNN010000000
VERSION     DAJTNN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.312"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>2210
                     /locus_tag="OO150_004153"
     CDS             <1..>2210
                     /locus_tag="OO150_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5518082.1"
                     /translation="KYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLLQPQFTVEQL
                     RELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQIALYRDARG
                     YVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPWRLRNYSTWT
                     RNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLASDDNMLPNS
                     QRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSGDLEVTIEES
                     DGTQRRFIQPYSSLPMMQRPRHLKYSATAGRYRADANSDSKDPEFAEATAIYGLNNTF
                     TLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGYQWRTQYIKD
                     IPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTIFDGVSLYAS
                     GSQQDYWGNNEKNRNISVGVSGLQWGIGYSLNYQYSRYTDENNDRALSLNLSIPLERW
                     LPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHNSSLNASYRS
                     PYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDANGASGVRIQN
                     YPGIATDPFGYAVIPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFN
                     ANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISSKSQSWT"
BASE COUNT          611 a          538 c          514 g          547 t
ORIGIN      
        1 aaatacgcac aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt
       61 tcacagaaaa aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg
      121 gtagaacaac tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa
      181 gatgacgata gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc tgctgaattt
      241 gatttcaatc atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca
      301 agaggttacg tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac
      361 tcgtttacag gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg
      421 aatatgcaaa atggtgctaa ttttggcccc tggcgattac gtaactattc tacgtggaca
      481 cgcaacgatc aggcgtcaag ctggaacact atcagtagtt atttacaacg tgatatcaag
      541 gcgttgaagt ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc
      601 tacaccttta ctggcgtgca actcgcttcc gacgataata tgttgccaaa cagccagcgc
      661 ggatttgccc caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa
      721 aatggttatg tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc
      781 tacccctctt ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacacaa
      841 cgtcgcttta tccagcctta ttcttcatta cccatgatgc agcgacctag gcatctaaaa
      901 tatagcgcga ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa ggaccccgaa
      961 tttgctgaag ccacggcaat atatggtttg aataatactt ttacgctgta tggcggcctg
     1021 ctcggttctg aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg
     1081 ggcgcgttgt cgatggatat caacagagct gacacccaat tcgataacca gcactctttt
     1141 catggctatc aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc
     1201 gctgttagct actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc
     1261 cgcaattggg actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca
     1321 atatttgatg gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac
     1381 gagaaaaaca ggaatatctc tgttggggtt tccggcctgc aatggggaat tggttacagc
     1441 ctgaattatc aatacagccg ctacactgat gaaaataatg accgtgcact ctctttgaat
     1501 ctcagtattc cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc
     1561 cagaaagatc gcccaaccca acatgaaatg cgtcttgatg gctcactgct ggatgatggt
     1621 cgcctgagct atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg
     1681 aacgccagtt accgttcacc ttatggaacc ttcagtgccg gatacagtta cggtaatgac
     1741 agcagccaat acaattacgg cgttaccggc ggcgtggtta tccatcctca tggcgtgacg
     1801 ctctcgcaat atctgggcaa cgcttttgcg cttatcgatg ctaacggggc ttctggcgtg
     1861 aggatacaaa actatccggg gattgctact gatccctttg gctatgcagt gattccttat
     1921 ctcacgactt atcaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc
     1981 gatcttgaac aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt
     2041 ttcaacgcca atatcggtta tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg
     2101 ttgccctttg gcgctcttgc cagcaacgat gatacggggc aacaaagtat cgtcgatgag
     2161 ggcggcatac tatatctctc tgggatatcg agtaaatcac aaagctggac
//
LOCUS       DAJTNN010000303         2181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTNN010000303 DAJTNN010000000
VERSION     DAJTNN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.313"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            131..1747
                     /locus_tag="OO150_004154"
     CDS             131..1747
                     /locus_tag="OO150_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5518083.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(1803..1955)
                     /locus_tag="OO150_004155"
                     /pseudo
     CDS             complement(1803..1955)
                     /locus_tag="OO150_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1978..>2181)
                     /locus_tag="OO150_004156"
     CDS             complement(1978..>2181)
                     /locus_tag="OO150_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518084.1"
                     /translation="IGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT          636 a          375 c          566 g          604 t
ORIGIN      
        1 ttttattaag cttaaacagg aagatgttga gcttaggcgc tggcgattat taagaagtgc
       61 aacgttatct aaagagcgga taactgagga agcacaaaga ttcttggaaa tggtttatgg
      121 ggaagagtga gtggttaggc tagctacatt taatgacaat gtgcaggttg tacatattgg
      181 tcatttattc cgtaactcgg gtcataagga gtggcgtatt tttgtttggt ttaatccaat
      241 gcaagaacgg aaatggactc gatttactca tttgccttta ttaagtcgag ctaaggtggt
      301 taacagtaca acaaagcaaa taaataaggc ggatcgtgtg attgagtttg aagcatcgga
      361 tcttcaacga gccaaaataa tcgattttcc taatctctcg tcctttgctt ccgtacgcaa
      421 caaggatgga gcgcagagtt catttattta cgaagctgaa acaccatata gcaagactcg
      481 ttatcacatc ccacagttag agctagctcg gtcattattt ttaattaact cctatttctg
      541 tcgaagctgt ttgagcagta ccgctttaca gcaagagttc gacgttcagt atgaggttga
      601 gcgagatcat ttagagataa ggatcttacc cagttcatcg tttcctaaag gggcgttaga
      661 gcagtcggcc gtagtgcagc ttttggtttg gttgttttcg gatcaagatg ttatggattc
      721 gtatgaaagt atttttaggc actatcaaca aaatagagaa attaagaacg gcgttgaaag
      781 ctggtgcttt agctttgacc ctccgcccat gcagggttgg aaattacatg taaaaggacg
      841 ttcttctaac gaggataagg attatttagt tgaggaaata gtaggtttag aaatcaacgc
      901 tatgcttcct agcacaacag ctattagcca tgcctctttt caggaaaagg aggcaggtga
      961 tggtagtacg cagcacatag cggtttcaac agagtcagtt gttgatgatg agcatctaca
     1021 gttggacgat gaggaaacag ccaatataga cacagacaca cgagtcatag aggctgagcc
     1081 gacatggata agttttagta gacctagtcg aattgaaaaa tctcgcaggg caagaaaaag
     1141 tagccaaact attttagaaa aagaagaagc aacaacaagt gaaaatagta atttggttag
     1201 tactgatgag ccacacttag gtggtgtcct agcagcggca gatgtgggtg ggaagcagga
     1261 tgcaaccaat tacaactcta tttttgctaa tcgatttgct gcttttgatg agctactttc
     1321 aattctaaaa actaaatttg catgtcgggt gctttttgaa gaaaccttgg ttttgccaaa
     1381 agttgggcgt agccgattac atctgtgtaa agatggctca ccaagagtga ttaaagccgt
     1441 tggggtgcaa cgtaatggca gtgaatttgt attgctagag gtggatgcat cggatggggt
     1501 gaaaatgctt tctaccaaag tgttgagtgg cgttgatagc gaaacatggc ggaatgattt
     1561 tgaaaagata cggcgtggag tggtgaagag ctcattgaat tggccaaata gtttgtttga
     1621 tcaattatat ggacaagacg ggcatagagg ggtgaatcat ccaaaggggt tgggggagct
     1681 gcaagtatcg agagaggata tggaagggtg ggctgagaga gtggttagag agcaatttac
     1741 gcattaaagg aatgactgaa agagcctgta aacccttttg tgtaagtgct tttgccggtc
     1801 agttaaaggt ggccatttaa acggtcacca aattcgatca taaaacggtt catggccggc
     1861 ttccagttgc ggatcggcat cgtccatttc ttggtcgccg cctggatagc caggtacacc
     1921 accttcatcg ctgattcgtc cgtagggaac accttgagtt catattaatg gaattttcta
     1981 caaatagcct ccgtggtttt gaggggggat tacagacgat ccatagtagt aatccaatga
     2041 gttcttgagc gcggcgacat gtttggacgc cttggcaaaa attagagcct gcttgaagtg
     2101 caggcgagca cgtgcttgga cgattgatcc attcgcggtc aaaaactcaa tcttggatga
     2161 cagcgtgtca ggaaatccaa t
//
LOCUS       DAJTNN010000304         2095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTNN010000304 DAJTNN010000000
VERSION     DAJTNN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.314"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..116
                     /locus_tag="OO150_004157"
     CDS             <1..116
                     /locus_tag="OO150_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5518085.1"
                     /translation="VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            113..463
                     /gene="tnpB"
                     /locus_tag="OO150_004158"
     CDS             113..463
                     /gene="tnpB"
                     /locus_tag="OO150_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5518086.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            494..2086
                     /locus_tag="OO150_004159"
     CDS             494..2086
                     /locus_tag="OO150_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5518087.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNSRKVHTDDTPVKVLAPGRKKAK
                     TGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLSPFRGILQADAFNGYDRLF
                     SAEREGGALTEAGCWAHARRKVHDVYISTKSATAEEALKLIGELYAIEHEIRGLPVSE
                     RLAVRQMQSKPLLTSLYKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFSDDGLAEA
                     DNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRYILSV
                     LPEWPSNRVDELLPWNVALTNK"
BASE COUNT          527 a          529 c          602 g          437 t
ORIGIN      
        1 cagttagtgc cagctcctgc aaggtggagt tccgtcacgg taacatgacg ctggaaaatc
       61 cttcaccaga gctgctcaca gtgttgatcc gtgaactgac cgggagggga cgatgatctc
      121 actcccgtca ggcacccgca tctggctcgt tgctgggata accgatatgc gtaagtcttt
      181 caacgggctg ggtgaacagg tacagcatgt gctgaatgat aatcccttct ccggtcacct
      241 gttcatcttc cgtggccgac ggggtgacat gattaaaatc ctgtgggctg atgctgatgg
      301 tctgtgcctg ttcaccagac gcctggagga aggccagttt atctggcctg ctgtgcgtga
      361 cggcaaggta tccattaccc gctcgcaact ggcaatgctc ctcgataagc tggactggcg
      421 tcagccaaaa acatcccgcc ttaatgcact gacaatgttg taaaaatgtc atggccggat
      481 tataaaaacg gccatgaatc aaaaatacct cattcgcatt gcagaactgg aatgccagct
      541 ccgtcagaaa gaccagcaac tgagtctggt tgaagagacg gaggccttcc tgcgctctgc
      601 actggcccgc gccgaagaaa agatcgaaga agatgaacgg gaaatagaac atctgcgggc
      661 tcagatagaa aaactgcgcc ggatgctgtt cggtacccgt tctgaaaaac tgcgtcgtga
      721 agttgaacag gctgaggccc tgctgaaaca acgcgaacag gacagtgatc gttacagtgg
      781 gcgggaagac gatccgcagg ttccccgcca gttgcgacag tctcgtcatc gtcgcccgtt
      841 accggagcat ctgccccgcg aaataaatcg cctggagcca gaagaaagct gttgcccgga
      901 gtgtggcggt gagctggatt atctggggga agtcagcgca gaacaactgg aactggtgag
      961 cagcgctctg aaagtgatcc gcacagaacg ggtaaaaaaa gcctgtacaa aatgtgactg
     1021 catcgttgaa gcaccggcac catcccgtcc gatagagcgt ggtatcgcgg gcccggggtt
     1081 acttgcccgc gtgttaacgg gaaaatactg cgaacacctg ccactgtatc gtcagagtga
     1141 aatttttgcc cgtcagggtg tcgaactgag ccgtgcatta ctctccaact gggttgacgc
     1201 gtgctgccag ttaatgacgc cgctgaatga tgctctgtac cgttatgtaa tgaacagccg
     1261 caaagttcac actgatgaca caccagtaaa agtgctggca ccgggcagga agaaggcgaa
     1321 aacaggatat atctggacgt atgtccggga tgacaggaat gccggttcgc cagagcctcc
     1381 ggcggtctgg ttcgcctact caccggacca tcagggtaaa catccggagc agcaccttag
     1441 tcccttccgg ggtatcctgc aggcagatgc gtttaatggt tacgatcggc tgttcagtgc
     1501 cgaacgagaa ggcggcgcgt tgacggaagc aggatgctgg gctcatgcgc ggcgcaaagt
     1561 ccacgatgta tatatcagta ccaaaagcgc gacagcggaa gaagccctga aactaatcgg
     1621 tgagctgtac gccatcgagc acgaaatacg cgggttgccg gtgtctgaac gcctggcggt
     1681 caggcaaatg cagagtaaac cgctactgac ttccctgtat aagctgatgc aggagaaaga
     1741 acacacgtta tcgaaaaaat gccgtctgag agatgcgttc cggtatatca ggaagcactg
     1801 ggttgcgttg tgcaacttca gtgatgatgg tctggctgag gcggataata atgccgcgga
     1861 aagagcgctt cgtgcagtct gtctcggaaa gaaaaacttt atgttcttcg gcagcgatca
     1921 cggtggagag cgtggtgcgc tactgtacgg gctgatcggc acctgccgac tgaacggtat
     1981 cgatccggaa gcgtatctgc gctatatcct gagcgtactg ccggaatggc cttccaaccg
     2041 tgttgacgaa ctcctgccat ggaacgtagc actcaccaat aaataagcgt caata
//
LOCUS       DAJTNN010000305         2074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTNN010000305 DAJTNN010000000
VERSION     DAJTNN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.315"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>2074)
                     /gene="pdeF"
                     /locus_tag="OO150_004160"
     CDS             complement(<1..>2074)
                     /gene="pdeF"
                     /locus_tag="OO150_004160"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5518088.1"
                     /translation="PIFAHINTFAHISSGEVFLFYLPLALMISMMMFFSWAALPGIAL
                     GIFVRKYAELGFYETLSLTANFIIIIIIIIIIILCWGGYRVFTPRRNNVSHGDTRLIS
                     QRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLINYQALLVGNLIGV
                     PLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLALGALLLLLCMPLNEKS
                     TIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIPIYPGYTTQLT
                     ITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLPNVRALNRALRDAPWS
                     ALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTE
                     SHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSI
                     VTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEIL
                     LRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLS
                     PTSVCQARFPFEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIA
                     IDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVA
                     EYVENEEIR"
BASE COUNT          618 a          490 c          474 g          492 t
ORIGIN      
        1 cgcggatctc ttcgttttca acgtactctg ccactacccg cattttcttc attcgcgcca
       61 ggtggcaaat cgatgccact atctgataat ccagactatt tgacacaata ttgcggataa
      121 aactgccgtc aattttaagc agatcggcat tcacattttt aagccgtgca tagctggcgt
      181 agccggtgcc gaaatcatca atcgcaatct ggcagcctaa ttcctgaaga tgctgcaagg
      241 tgatttgcgc ctgcttaaca ttggtcagag cattactttc ggtgacttca aaaataagtt
      301 gccacgcttc aatctgatat ttagccagca actgactgac ttcaaaagga aaacgagcct
      361 gacataccga ggttggagac agattaatag caaaacggtg agcgggcatc ttcgctctgt
      421 tttcagccat aaattgcagc gtatgctcaa tgacccacat gtcgatactc gacgataaac
      481 caaattcgtg cgcgaccggc aagaagctat cggggctgat cagttcatca ttctcacctt
      541 tcatgcgcag aagaatttca tggtaaacat caccacgcat accggtaatc ggctgggcca
      601 tcaggaaaaa atggttgtgt tccagcgcct gctgtagtcg attcatcatc gcgactttat
      661 ctttcaattc gcgttgcaaa tacattgccc cgcgacgctg catattttcc ggggcgttgg
      721 tcacgatgga aagttcggcg accgtattta gctctcccag cagcaggtag atatgattca
      781 ctggcgagcg cacatagcag taactgacgc caatctgcgg ttgcatcggc atgccatccc
      841 aaaagaaacg aaattgcttg agatggctat ccagtgcggt aatgcgctcc tggtgcgatt
      901 ctgtattcag tcgcagcgcg agatcgttac ccgaaagctg ataaacatct tcacccggtt
      961 ccagcaaggg tgacagccag tgagaaagtt tttgcttgta ttgaatccgc agcatgatgc
     1021 catagttctt aaccagcatt tccatgccag ggatgcgtaa ataacaaagc gcagaccagg
     1081 gggcatcacg caacgcgcga ttcagggcgc gaacatttgg cagatgaacc accgggtcca
     1141 catacgcaag ccgctgaatg cgtctgacta ccgctctctg acgggttgcc agtacagcca
     1201 tataattgac aataaaagag aataccagat aactggagga ggttatggtc agctgcgtgg
     1261 tatagccagg ataaatggga atgtaatttt gatagctgtg gatgctgatc atcaacacga
     1321 ccgcccagag cagagaaatc agcttataac catagcgcat cgctccccac atcatcaggg
     1381 gcagcaataa tgacaaggta taattggtgc taaaaattgt gcttttttca tttaacggca
     1441 tgcataacag caacaataaa gcacctaatg ccagtagcca gagcgcgaac tcttttttgg
     1501 tgactttggc atcaacctgc tgttttaatt gcgaataata gctacgtaaa taaaatggat
     1561 ttcgcactac ccgaatgatg aagtagcaca gcgggacacc gatcagatta cccaccagta
     1621 aggcctgata attgattaaa gtcccgaggt taaaaggcat gacaccgacc agattttctc
     1681 tgctcgccaa taatcctaca aatgcagcaa actggaaaag tatcagaaac agcgttgcag
     1741 gaaacacaat ctgccagaat atacgctggg aaattaaacg ggtatcacca tgtgaaacgt
     1801 tgttacgccg gggagtaaag accctgtaac cgccccaaca gagaatgata atgataatga
     1861 taatgataat gataataaaa ttagccgtta atgatagcgt ttcgtaaaaa cccagctctg
     1921 catatttgcg gacaaaaatc cctaaggcga tccctggcaa tgccgcccag ctgaaaaaca
     1981 tcatcatgct gatcatcagt gccagaggca gataaaagag aaaaacctca ccggaagaaa
     2041 tatgcgcgaa agtattaatg tgggcgaaaa tggg
//
LOCUS       DAJTNN010000306         2016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTNN010000306 DAJTNN010000000
VERSION     DAJTNN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.316"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(50..234)
                     /locus_tag="OO150_004161"
                     /pseudo
     CDS             complement(50..234)
                     /locus_tag="OO150_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(396..470)
                     /locus_tag="OO150_004162"
     tRNA            complement(396..470)
                     /locus_tag="OO150_004162"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(435..437),aa:Arg,seq:cct)
     gene            complement(546..1478)
                     /locus_tag="OO150_004163"
     CDS             complement(546..1478)
                     /locus_tag="OO150_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5518089.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1772..>2016
                     /locus_tag="OO150_004164"
     CDS             1772..>2016
                     /locus_tag="OO150_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HCR5518090.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPA"
BASE COUNT          490 a          487 c          484 g          555 t
ORIGIN      
        1 ccatctttta cggggtgaag gtagatttcg tatgaagggt atctgttgtc tcctgcaaag
       61 tcataagcgg atattttcct acggtcagta ttttttcctt accgtcaatc ttgtagcgaa
      121 gttgccatac cttttcccgg atacaggaac ataaaggtac aggccattac catcgagaag
      181 gcggtatggt ttttctttcg gctttgctgc ttcaatctgc ttaacggtga gcatgggtaa
      241 aaatccggtg ggtaaaatta ttttatccac tttttacccg tcatggagtg cggctgtcaa
      301 cgatctgacg cgaaccatga cgaaccgtga atatacggaa ggcttgatat tcaggggatt
      361 ttgcggactg gtacggatgg gagcgaactg ataaatggtg tcccctgcag gaatcgaacc
      421 tgcaattagc ccttaggagg ggctcgttat atccatttaa ctaagaggac aatgcggcat
      481 gagtataccc gctaatggac tgcggagtaa gtacactgcc gctcgattgc ttaaaccctc
      541 gccatttatg ctgggttttt atcatttttc ttaatgtttt ccgcacgttc tgctttttgg
      601 cgtgcttctg ctttgcgctt gttgctcatg tcgttacgga tctgtgcatg actcattaac
      661 gcgaagataa aggtgccgcc gcagatattc cccgctaaag taggtagtgc gaagggccaa
      721 atgaaatcgc tccagtgcag cgtgccgtta aacaccagat agaggatttc tacagaaccg
      781 acaacgatgt gggtggtgtc acccagtgca ataagccagg tcatcaatat aatcaccaca
      841 atctttgccg cacccgctgc aggaaacatc caaaccatag tggcgatcag ccagccggaa
      901 atgatcgcgt tggcaaacat ctcgctgggg gtgttcttca tcacatccat gccgattttg
      961 acaaatgcat cgtgagtttc ttcattgaag ataggcatat attcaaatgc ccatgcagca
     1021 atacctgtcc cgagaatatt acccagcagc acgacgcccc ataatcgcat aagtaagccg
     1081 acgttgctca ttgtcggttt ttgcatgacg ggtagtaccg cagtcacggt gttttcggta
     1141 aataattgct ggcgggccat aatgacgata ataaaaccaa aggtataacc gagattctcc
     1201 aacaagaagc tgcccggcac gccttccagt tcgacatgaa atatcccttt tgccagtaac
     1261 gaagcgccca tcgacagacc cgccgcaatg gctgaccaca gtagtgccat tgcgtcgcgt
     1321 tccagctctt tttcaccatc ctggcggata tgttcatgaa ttgccatcgc ccgggagggg
     1381 agtcgatctt catctatttc tatttttttg ccgcgctctt tttcttcgct ctcaacttca
     1441 atttcgtcgc tgtgttgatc aattttgtcg ttgtccatgg tctcttctct tgaattagca
     1501 cgtatagcta aagcgtagcg gcttttttgc tcgcaactgg cgggagttac tctgaaaatg
     1561 tagaaaaggc cgcgtttgcc tttttctgtt tctatagaat caagtagcct acagggcggc
     1621 gattaccagg ctatgatcaa atcagcaaat cagggcgtct ggacatcagt tgacgtgctg
     1681 ttacaatcgc ccacacctaa acaggcggat acggtatcgt tccgtcatgg atggcaaact
     1741 gcataagcca taaaaaaaca gggagacatt tatgaagctt cgcctgtcgg cgcttgctct
     1801 gggaactacg cttctggtgg ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga
     1861 cccgttagaa gggttcaacc gcaccatgta caacttcaac ttcaatgtat tagacccgta
     1921 tattgttcga ccggtcgctg tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaacgg
     1981 tttgagcaac tttaccggca accttgaaga acctgc
//
LOCUS       DAJTNN010000307         1979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTNN010000307 DAJTNN010000000
VERSION     DAJTNN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.317"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..447
                     /gene="yeeE"
                     /locus_tag="OO150_004165"
     CDS             <1..447
                     /gene="yeeE"
                     /locus_tag="OO150_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5518091.1"
                     /translation="VLIGLIALLAWPLSEATGRMFGLGITSPTANILQFLVAGDVKYI
                     NWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIGN
                     GLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            461..688
                     /locus_tag="OO150_004166"
     CDS             461..688
                     /locus_tag="OO150_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5518092.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(731..>1979)
                     /gene="sbcB"
                     /locus_tag="OO150_004167"
     CDS             complement(731..>1979)
                     /gene="sbcB"
                     /locus_tag="OO150_004167"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5518093.1"
                     /translation="PGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNN
                     VRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGLP
                     SFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMAL
                     IDVPQMPPLVHVSGMFGAWSGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDT
                     LRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLKI
                     LRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPAL
                     DITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQ
                     QYADDKEKVALLKALWQYAEEIV"
BASE COUNT          468 a          493 c          514 g          504 t
ORIGIN      
        1 gtactcatcg gtttgattgc gcttttagcc tggcccctga gtgaagcaac cggacgcatg
       61 tttgggttag gaatcacttc cccaacggcc aatattctgc aatttctggt cgcgggtgac
      121 gtgaaataca ttaactgggg cgttttcctg gtgttaggga tcttcgtggg gtcatttatt
      181 gcagccaaag cgagccgtga gttccgcgtt cgcgcagctg atgcacaaac aacattacgt
      241 agcgggttag gtggtgtact gatgggcttc ggtgccagta ttgcaggtgg ttgctctatc
      301 ggtaatggac tggttatgac tgcaatgatg acctggcagg gctggattgg ccttgtattt
      361 atgattctcg gagtctggac tgcatcctgg cttgtgtatg ttcgaccgca gcgtaaggcg
      421 cgactggcga ctgctgcggc aaattaagta aggtgtaaag atggcgatta aaaagctgga
      481 tgtggtgacg caggtctgtc cgtttccact catagaagca aaagcagcac tggcagaaat
      541 ggccagcggc gatgagttgg taattgagtt tgattgcacc caggcaactg aagcaatccc
      601 acagtgggcg gcagaagaag gacatgccat caccgattat cagcagattg gcgatgcagc
      661 gtggagcatc accgttcaaa aagcctgact caacattgtc ctccgccgta ccagcggcgg
      721 aggcttcaaa ttagacgatc tcttccgcgt actgccaaag tgcttttaac agcgccactt
      781 tttctttgtc atcggcatat tgttgtacca gcatctgcaa ttcatcagca taaccctgca
      841 aaaactctgg cgtgaatacc tggcggcggt gctccagcca gcgttgctgc tcggcataat
      901 ccagcgtccc cgggaagttg cgtgcccgat aattgaacaa cagcttttca atccgtttat
      961 caacaaaagt gatatccagt gccggtaaat tacgtggctc ggtttccagc acaattttca
     1021 ttgctgcacg atctgcgtca ctgaaaaagc cgttataaag ctgtgcatcc acgttatctg
     1081 taggcgtaaa cggttcggct tccgcgaata tcgccaccac tttttcacgc acttgcggat
     1141 tttcacgcag aattttcagg ttatcgaggc aatgctgacg attaattccc agtcggtcgg
     1201 catcttccgg gcgtagcgta ttcgcctggg ccagcaccgg acatttattg atgtgcacca
     1261 gcttaaccgg aacggcggcg ttatcgccaa gatcggtttt tgcggtatat aaacgctcgc
     1321 gcaatgtgtc gctatccagt tccagtaatg gcgaaatgtc tcctgccaaa tccaccataa
     1381 ttacagcatt gcgattttca ggatgccacg ccagcggtgc cacccagctg gtattgccgc
     1441 tccatgcccc aaacattccg gaaacgtgaa ccaggggggg catctgcgga acatcaatca
     1501 acgccatcag tttgtgttta ttacgatggg taaagagata atcaaacagg cgtggctgac
     1561 gcgtttttac cagtttcgcc atcgcaatag tggcgtacac atcagccatc gcatcgtggg
     1621 cgttgctatg ttcaatacca ttcgctttgg ttaaatgctc aaggcgaaag ctcggtagac
     1681 cgtcatcatt ttcaggccag tttattcctt ccgggcgcag ggcataacag gcacgcataa
     1741 catccaacaa atcccagcgc gagttatcat gctgccagct ccaggcgtaa ggatcgtaga
     1801 aattacgata aaaaacgttg cgtgtgactt cgtcgtcgaa acgcacattg ttgtagccca
     1861 gaatacaggt cttcggtacg gtaaaaagcg agtgaatacg ggcggcaaac gcggcttcgt
     1921 tttctccttt cgcccgtgct tcctgcgggg taataccggt aattaatact gctccaggc
//
LOCUS       DAJTNN010000308         1937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTNN010000308 DAJTNN010000000
VERSION     DAJTNN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.318"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="OO150_004168"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="OO150_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5518094.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1149..1520)
                     /locus_tag="OO150_004169"
                     /pseudo
     CDS             complement(1149..1520)
                     /locus_tag="OO150_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1519..1937
                     /locus_tag="OO150_004170"
                     /pseudo
     CDS             1519..1937
                     /locus_tag="OO150_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          611 a          347 c          386 g          593 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc cttttggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ctagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac
     1261 gactggagtt gcttctgcgg tgctctggtg gcgaatgtga agtcatactc ttcgaacgtt
     1321 ttcaccgccg ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc
     1381 agtttttctt catgaagcag atgctccagg aagtccatat aactccattc ctggtctact
     1441 gcctgttgtg acagcgcagg cgctgcgctt atgtactgca cccattttgt tggacgatga
     1501 aatggaatag cccctaatat gtcaaagcca aaataccctt ttgaaaagcg ccttgaagtc
     1561 gtgaatcact acttcactac tgatgatggt tacaggatca tctcggcacg ttttggtgtc
     1621 cccgaaccca ggtcaggaca tgggttgccc tctatgaaaa acatggagaa aaaggcttaa
     1681 ttcccaaacc taaaggtgtt agtgctgatc cagagttgcg tattaaggtc gtgaaagctg
     1741 tgattgagca gcacatgtcc cttaatcagg ctgctgctca ctttatgctt gctggtagtg
     1801 gttctgtagc caggtggctg aaggtttatg aagagcgcgg agaagctggt ttacgcgcgc
     1861 tcaagattgg caccaaaaga aacattgcaa tatcagttga tccagaaaaa gcggcatcag
     1921 cattggagct gtcaaaa
//
LOCUS       DAJTNN010000309         1909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTNN010000309 DAJTNN010000000
VERSION     DAJTNN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.319"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            225..518
                     /locus_tag="OO150_004171"
     CDS             225..518
                     /locus_tag="OO150_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5518095.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            590..1249
                     /locus_tag="OO150_004172"
     CDS             590..1249
                     /locus_tag="OO150_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5518096.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1341..1787
                     /locus_tag="OO150_004173"
     CDS             1341..1787
                     /locus_tag="OO150_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5518097.1"
                     /translation="MNDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          505 a          409 c          501 g          494 t
ORIGIN      
        1 gaatgagtac gatgaactgt tataatataa caatccctaa cgggagaatt ccagtggctt
       61 ttctgcgttc ggtaaaatcc cggcgcggca ttttttgccg gtgggattac ttataaaggg
      121 gcatggactc aacaaaccgc agaaatctac agctggctgg cttgtggcgc gggtttcatt
      181 gatgttagct tatgccaaaa cctggcatct taaaaagtaa aagtatgttt tgtgtgattt
      241 atcgaagcag caagcgtgac cagacctatt tatatgtcga aaaaaaagac gatttttcgc
      301 gtgttcctga ggaactgatg aaaggttttg gtcagcctca gttagcgatg attctgccgt
      361 tggatgggcg gaagaaactg gtcaatgctg atattgaaaa ggttaaactg gcattaaccg
      421 agcaaggtta ctatttgcag ttaccgccac cacccgaaga tttgctgaag caacatcttt
      481 ccgtcatggg gcagaaaaca gacgacacga acaaataacc gatatccggc ggtggcatta
      541 tctttgtcgg cgcggctttt catatccacg ataaggtgag gggaacgtta tgtatcaaca
      601 tcacaactgg caaggtgcgc tgctggatta tccggtgagt aaagtagtct gtgttggcag
      661 taactatgcc aaacatatta aagagatggg cagcgcagtg cccgaagagc cagtgctgtt
      721 tattaaacca gaaacggcac tgtgcgatct gaggcagcca ttggcgatcc catccgattt
      781 cggttcagtt catcatgaag tcgaactggc ggtgttgatt ggtgcgacgc tgcgtcaggc
      841 aacggaagag catgtccgca aagccattgc cggttacggc gtggcgcttg atctgacatt
      901 gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg tgggaaaagg ctaaagcgtt
      961 tgataactct tgtccgcttt ccgggtttat ccccgcggcg gaatttaccg gcgatccaca
     1021 aaatacaacg ctgggcctga gcgtaaacgg cgaacaacgc cagcaaggta cgactgcgga
     1081 catgatccat aaaatcgttc cgttgatcgc ttatatgagc aagtttttta ccctcaaggc
     1141 cggtgacgtt gtgttgacag gcacgcctga tggcgtcggc ccgttgcaaa gcggtgatga
     1201 gctgacagtc actttcgatg ggcattcttt gacaactcgc gttttgtaat actttttgcc
     1261 gcctgaaagc ggcggcaaaa cttgcatcgc tgtgccagac tggttataag gtgcgtttta
     1321 acgtaatggc ggaacacctg atgaacgatg tacctttctg gcaaagtaaa accctggacg
     1381 aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg cggtcagtgt tgcctgcata
     1441 aactgatgga tgaagacacc gacgaaatct acttcactaa cgtcgcctgt cgccagctca
     1501 atattaaaac ctgtcaatgt cggaactacg aacgtcgttt cgagtttgaa cccgactgca
     1561 ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct gccaatgacc tgcgcttatc
     1621 gtttgctggc ggaaggtaaa gatttacctg tgtggcatcc gctacttact ggttcgaaag
     1681 cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc agtgaaagaa tcagaagtga
     1741 ttgactggca ggatcatatc ttaaataaac ctgactgggc gcagtgataa atttaataga
     1801 tgttgatggg ggctttctta atatacgtct ggatgctcgc catttcgtag ccctgttttt
     1861 gaaagcgctc tgtcataatg agagttaccc gataccttat tatcaacag
//
LOCUS       DAJTNN010000310         1894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTNN010000310 DAJTNN010000000
VERSION     DAJTNN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.320"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..918)
                     /gene="virK"
                     /locus_tag="OO150_004174"
     CDS             complement(<1..918)
                     /gene="virK"
                     /locus_tag="OO150_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5518098.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSSSGFDREGELMLSLFYNKTVITRLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAY"
     gene            complement(923..>1894)
                     /gene="capU"
                     /locus_tag="OO150_004175"
     CDS             complement(923..>1894)
                     /gene="capU"
                     /locus_tag="OO150_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5518099.1"
                     /translation="NSKIAFEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAI
                     VCHSGHDSNIVGLVRLFTWKHPFRIIRQKTYLTQKTKVFSINHFCDEVIVPGTSMKTH
                     LEQEGCRTRVTVVPPGFDFQKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKG
                     HEFMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAP
                     VYRVASLVVLPSENESFGMVLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKH
                     AWMCALNDFFNDPGRFYQMARQAKQDIEERFDINKTALKILTLAKHK"
BASE COUNT          575 a          434 c          369 g          516 t
ORIGIN      
        1 atatgcatcc aaagagcgga tagtagtttc atggatataa tccagcaaag cgtatcgttt
       61 tcggtactca gagcgttttt tactggctat atcgcttagc ggctttcgaa tcacctgggt
      121 tggtaatctg taccagtcac cacaggtttt tcctgctaca gactcccaga aatcactata
      181 gaccgcaaca aaggttttgc gtttctgata ccagtatctc aattgccgga aaacatgact
      241 gtgctcactg actgccagac actcagagat attgcaagct ttcatcaacg cacaaaatgc
      301 ttcaaaaata atccgttttg ggaaaatccc ataacaggcc cgggttgcgc accgaataac
      361 atcaggtccg gtatttttag gggctccctg taatccgcca ataaaagcaa tgtgtccatt
      421 ttgagtcaga atgactgaaa atgttaaccg ggtaataact gttttattgt aaaacaatga
      481 caacatcaac tctccttccc ggtcaaaacc actggaagaa cactggatat caaaattctc
      541 tccatctttc ccgatgaatt ggactaatga taccgatttt tgtggaaaca aaaatttaga
      601 atatttttct ggcaaatttt gcacaaagcg ataatgctca agaatatatt gtccacgggt
      661 ccaggcgcgc ccccctttat gcaaataagg gcgatgaatt cttgctggca acgtggggtg
      721 tattgccaga atatcctcaa tgcacggcaa ggaacgcagt tcgtaataat atcgaatact
      781 ggcaaccgga tttatagcac atcgcaaaat gtacatgaac cggaattttc tgtgttccca
      841 cttccccggg agtgaatctg aggaaaaaac aatcctttta aggaaaccga taaatgataa
      901 gttacttaca gaaaacatat tgttacttgt gcttcgctaa tgtgagtatt tttaacgcag
      961 ttttattaat atcaaaccgc tcttctatat cctgttttgc ctggcgagcc atctgataaa
     1021 aacgcccagg gtcattaaaa aaatcattca gggcgcacat ccatgcgtgc ttattacctg
     1081 ctggtaacaa tgtcccggtc tggttgttct gaataacatc agggattcca ccaatctgac
     1141 tggccagtac aggcacagaa aatgccgatg cttctgccag taccatacca aacgattcgt
     1201 tttctgaagg cagaaccacc agactggcaa cccgatatac gggggcggca ggaaaaacat
     1261 tgtcagcaat aaaaacatca tcatgcatgc ccatactgtc aatctgatac tgtaaatgct
     1321 cccgcagttc aggcgaacca gaccccacaa tcaaccaaca gaactgtcgt ccattcattt
     1381 ttaaatggaa cagtaaattc aacataaatt cgtgcccttt ttccgggcgc aacattccta
     1441 cctgagcaat aacggggcat ccccttcggg acgccagcca agaaagaaca tttggaggca
     1501 aactgtttcg ggaatcaacg tataatttct ggaaatcaaa gcctggaggc acaacagtaa
     1561 cccgggttcg acatccttcc tgctccagat gtgtcttcat acttgttccg ggaacaatca
     1621 cctcatcgca aaaatgattt attgaaaaaa cttttgtttt ttgtgtcaaa tatgtctttt
     1681 gcctgataat tctgaatgga tgtttccaag tgaataaccg tactaaacca acaatattgc
     1741 tatcatgccc actgtgacaa acgattgcat tgggctgaaa accatgaact attccgagta
     1801 atctccatgc agtagggatg tgaagactgt ttcgaaataa cgcgaatgtg atatcaattc
     1861 ccaatttact ggcttcaaaa gcaattttgc tgtt
//
LOCUS       DAJTNN010000311         1885 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTNN010000311 DAJTNN010000000
VERSION     DAJTNN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.321"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            193..468
                     /locus_tag="OO150_004176"
     CDS             193..468
                     /locus_tag="OO150_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5518100.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            465..1022
                     /locus_tag="OO150_004177"
     CDS             465..1022
                     /locus_tag="OO150_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5518101.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1149..1319)
                     /locus_tag="OO150_004178"
     CDS             complement(1149..1319)
                     /locus_tag="OO150_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518102.1"
                     /translation="MVRLPPVVMSAKSATARTTIARENAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(1434..1703)
                     /locus_tag="OO150_004179"
     CDS             complement(1434..1703)
                     /locus_tag="OO150_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5518103.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            1772..>1885
                     /locus_tag="OO150_004180"
     CDS             1772..>1885
                     /locus_tag="OO150_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCR5518104.1"
                     /translation="MKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTK"
BASE COUNT          516 a          449 c          392 g          528 t
ORIGIN      
        1 acatgtttta aaaaatgcaa taagacattg tatctgaaag aaaaaacagg attttgaggc
       61 gcttttttat accgtttttc cgtgttttta actgcctgat tttattgaaa tcttttttag
      121 ttttgcagaa aggatttttt gctcgatcgc tttaatttta tacattcctg ataacggaaa
      181 tttttttgcc cgttgacagt ttttgttctc cggggctata ttcctcacac aaccgcaaaa
      241 tcggttgtcg ggattggcgt ctcggataac atcacaacgc atagccgcgt ttgcggtttt
      301 ttttgcgtta agcattgcca cattcgcatt atggtggggc gtgcaggggc ggagaaatcc
      361 gcgccgggtt ttgtgatgac cggttacgcc aaccctgtac gtctcaccac ctccctgatt
      421 ggcgtcagag gtggtgatca tccaattaat tggagtcatc acatatgaat acagctataa
      481 ctttttctaa cgagtcacca aaagtctttt tcaaagatgg tcgcctggtt accacctctc
      541 aagctgttgc cgactacttc gataaacaac ataaacacgt tttagctaag attgacacac
      601 tcgactgctc tcccgaattt acatcagcca acttttcggc cgatgttcag actgtcgaaa
      661 ttggcaacgg cgcagaacgt gaatcacgat gctacctgat cacaaaagac ggcttcatgt
      721 tcctcgtaat gggatttact ggcaaaaaag cagccagact aaaagaagct tacattgaaa
      781 aatttaacgc aatggaagaa gaactccaca aacgtcctcc tgcagcacaa aactcccccg
      841 ccccaaataa tggatgcgca ttactgatcc acttcgataa acacggtcag gtcgagttca
      901 cagaaaaagt ccccgccgat gcgatggtat gcacgctgga acggtttaaa ttttatctgg
      961 agcaacatgg gtggatcgtt gcccgtaaag aacaattggt ggaaaggttg atgcggtttt
     1021 aatgaatgca aaaccccgga tgatcaccgg ggttaatttt atcgagcgtg attgccgcta
     1081 atcgccatcg ctctgattga gcgtaaatca ttcaggttat tcagttcttc cttctgctct
     1141 cgctgacgtc aataaatccc atcattgcga tcaacctcat ccttaaccat tgatgcgagc
     1201 agttcttcca gtttctgcgc tgacagcttt acctggtgat cttcagcatt ttcccgggct
     1261 attgtggttc gcgcagtggc tgattttgct gacatcacca cagggggcag acggaccatt
     1321 gaaccatggc ctccggaaca aataagggca aaaataacaa ccactatcga aagctcacaa
     1381 actaaccgca gcacgttcct gcatacgacg tgtctgcggc ataatcccaa tgattactcc
     1441 ctgacaggat ttgcaggcca ctcaatatca ggtgcagttg atgtatcaac acgattcaac
     1501 aatacccgat atttattcca tgcctccagc aacgatcttt cttcctccgt tgcgatttcc
     1561 agatctacag catcctgcag tggcgcaata tactcactga attcctggat gtagaactgt
     1621 gtggtgacgg tcttccagcc attcggctcc tgctgtatcg aagcatacca ggctatttca
     1681 atatcgctat gctgcggcag catttaaccc cttgtaattc atcgccataa ttgatttaat
     1741 tcacaaataa aactataaca cggtgaaatc aatgaaaaaa aacacagatg atggggctaa
     1801 aatttacaca ccacttaccc taaagcttta tgactggtgg gttttgggag tatcaaatcg
     1861 gcttgcatgg ggatgtccta caaag
//
LOCUS       DAJTNN010000312         1845 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTNN010000312 DAJTNN010000000
VERSION     DAJTNN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.322"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..762
                     /gene="potA"
                     /locus_tag="OO150_004181"
     CDS             <1..762
                     /gene="potA"
                     /locus_tag="OO150_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5518105.1"
                     /translation="PRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIARAVVNKPRLL
                     LLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRIVVMREGRIE
                     QDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGRECNIYVNFAVE
                     PGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELENGKMVMVSE
                     FFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            746..1609
                     /gene="potB"
                     /locus_tag="OO150_004182"
     CDS             746..1609
                     /gene="potB"
                     /locus_tag="OO150_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5518106.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
BASE COUNT          425 a          443 c          476 g          501 t
ORIGIN      
        1 ccccgcgtga tggaagccct gcggatggtg caactggaaa ccttcgctca acgtaaaccg
       61 catcagctct ctggtggtca acagcaacgc gtcgccattg ctcgcgcggt ggttaacaag
      121 cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa
      181 atgcagaacg agctgaaagc gttacagcgt aagcttggca ttacattcgt cttcgtgact
      241 cacgaccagg aagaagcact caccatgtca gacaggattg tggtgatgcg cgagggtcgc
      301 attgagcaag acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc
      361 ggcttcattg gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag
      421 cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa
      481 ccggggcaaa aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac
      541 gacgacaacc acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg
      601 acgctggagt cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc
      661 aatgaagacg atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta
      721 gaaagctggg aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat
      781 tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg
      841 caccagcttt ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cgctggataa
      901 ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc
      961 gctgatcgcc accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa
     1021 gctgccacac aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa
     1081 ctcattgatt cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga
     1141 gtttttgctc tggctgggcg ttatcgacac accaatccgt atcatgttca cgcccagtgc
     1201 ggtgattatc ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtactccag
     1261 tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt
     1321 acagactttt atccgcatca ttattccact gacgatgccg gggattattg ccggatgtct
     1381 gctggtgatg ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa
     1441 aaacctgctg atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt
     1501 tggtgcagcc accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg
     1561 gcgcgcttct cgtttgctga ataagaaggt gagcgaatta gatgattaat catttatatt
     1621 caatgcatta agtttgtttt tgagtggttt tgccgtttaa ggtaacggcg ttatagatcc
     1681 tatcaaaatc aatcaattcc attctggttt tggagaaaga tttttcgaga gcttcggtct
     1741 ttaactaaaa ctgattgcgt ataaccccaa tacccctccc cgactatctg aatggtcggg
     1801 gaggcacttc acgcacaaaa tatcatccct gcgccgttga ttact
//
LOCUS       DAJTNN010000313         1740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTNN010000313 DAJTNN010000000
VERSION     DAJTNN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.323"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(891..1364)
                     /locus_tag="OO150_004183"
                     /pseudo
     CDS             complement(891..1364)
                     /locus_tag="OO150_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1292..1658)
                     /locus_tag="OO150_004184"
                     /pseudo
     CDS             complement(1292..1658)
                     /locus_tag="OO150_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          391 a          485 c          417 g          447 t
ORIGIN      
        1 tcaaaattat caaagcgcag aacctcagct tcttcttcgt caaaaaaaat catcttcgtg
       61 aagttttacg acatagtgga ggagtttgct tctttgaggt gcaggttttc gtttgcgtgg
      121 tttagctgaa cgggccatat aactacctga aagacaatga tattgtctgt ttttataacg
      181 gtaatcacag gccatgacaa gccgcagccg tcaggccgcc tactcggggc ggtttagaac
      241 gcttatttag taccagatct tacttataat aaaggggctt ggcagtttgg ataaactatt
      301 gtccttattc cactaataca aactttttgt aaagtatatt aaatatactt catttcatac
      361 tgaaatcccc ggaagtattc aatcaaacaa ttacaccata aaaggaagag ggggccccaa
      421 ttaagttgtc aagcatgttt actgcgcaat cgcttggtgc tccgtccctg taccccgcat
      481 tggtactctg gcgctcatgg ggcttccact tgcgcttctc ccttcacatc ggggtgacag
      541 gtccacgttc cccacaagag cctgaatcga cttcacgccg cttttatgcc ggatgccgcg
      601 caggcagtaa acatatccca caggaacgac tccaccattg ttttgacatc atctatatgc
      661 tttcgacaca tcatcagcgg ttcactttcg ttcgtctctt cgaaccatac ctgatcggct
      721 cacgtccgac cttttcccat ctcgctcacc accacatctc ttaaatgcag cagcagtgga
      781 tggtttggtg cctgcgcctg cacgccgaca ccgaggggcc ttccctcatc tcttgtgccg
      841 catggctgca cagggactca cgtccccagg acgcgccttc gtggcacaca gtcatcggca
      901 tagcaacagt aagcaacggc aggtttccat tgccagtttt ctttgacagc agtacttcgg
      961 cctcgctgga tactgtgatt ccagtaccag cgatcctttc tggcttttcc actcaggtag
     1021 cgtttattca gatactggtc gaactcattc agtatgatgt tcgacagcaa cggcgagatc
     1081 acaccgccct gtggcatact ttcactgacc gcccggaaga gaccaacatc aacacggcgt
     1141 tttccacagc agtctcataa aacgtgcatc actgatcctg cggcagacgg ctttcatcag
     1201 cagccggtga tgtactgtgt cgaagtagct ggacaggtca ccttcaataa cccggcgccc
     1261 cgtgtttccc cgcagtcggt gagctgtaat agtacgtgat gtctcccgcc cacttctggt
     1321 tcgggccact ggcgtaaaaa tcctgcgtca gcagattttc tgatacaggc aggccgtgtg
     1381 cacggtaact gatcgggctg aacttctggg aggcttttgc gctcagttcc tgacggcgca
     1441 gactggctgc cacggttttt acgttaaagt ggtaaccaga gcacacagtt catccgtcag
     1501 gcgtggggca ccgtaacgct ggactgggta aaagccttgc ggacaacgct gtcgcagtgc
     1561 tgacggaact gctgacgtgt gtttatcctt gtccgccgct gacaccacgt ataccagccg
     1621 ctgcgggcca cccggagtac gcggcacatt gccttgatga cggtttacgc ggtcgcctgc
     1681 ggcaacggct ttccggctga cggtagtgtg gaccttttta ccccggagaa caccggcaag
//
LOCUS       DAJTNN010000314         1708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTNN010000314 DAJTNN010000000
VERSION     DAJTNN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.324"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(17..889)
                     /locus_tag="OO150_004185"
                     /pseudo
     CDS             complement(17..889)
                     /locus_tag="OO150_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(903..1043)
                     /locus_tag="OO150_004186"
     CDS             complement(903..1043)
                     /locus_tag="OO150_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518107.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVSVFNDNESKITDAAVNLFI
                     QI"
     gene            1182..>1708
                     /gene="queE"
                     /locus_tag="OO150_004187"
     CDS             1182..>1708
                     /gene="queE"
                     /locus_tag="OO150_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5518108.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLA"
BASE COUNT          505 a          345 c          380 g          478 t
ORIGIN      
        1 attttttatg gttgagttac cagcggccgg atgcgccgcc acctccagaa gaaccgccac
       61 cgccgctaaa tccgccgcca cttgagcctc caccggaacc gctgttgtct gaatgataac
      121 ttttacctga tgctcttttc tgaagtacac ataaacaggt aaagaccata aatatggtga
      181 aagtaaaaaa gaaggaaaat acagcaatgg aaaaaatttt atcggagaac aggaataaat
      241 ctaaaagata gattgctgaa atacagactc cacttttaca tgcgcgacaa aaattactgc
      301 cttgatgaaa aaaccagaaa gggaagaaca tcactgcaaa tacccaaaaa atagcaaagt
      361 aataatgatc actggaagac gctgattcac tttctgaagg attagtcgga tattggtgtt
      421 gtgaagtgag ttggttattc aaagcgttta tagctagctc taatccctga gcaaattttt
      481 gttgtttaaa tgcgggtatc atgttgctac ggatgatatc tccagccaga gcatcggtta
      541 ccttttcttc cagcccataa cctacctgga tgcggacagt acgatctgac caggcaacaa
      601 tgatcagtat cccatcatta cgtttggcat ctcctaaacg ccaattgtca aaaactcttg
      661 tagcatattg ttcaattgtc tcgtctttgg ttgtgggaac aattaataca gcaatttgca
      721 cttgggtatt attgaaaata gattctaact tgtgcgtcag ttctttcttt tcttgtgagt
      781 ttaagctact cgtaagatcc gtaacttgtt actgtaattg tgggacaata gttggttggg
      841 ctgcaacaaa ggaacatacg aaagtgaaca ttagtatgaa atagcgcatt ataaatttct
      901 cattaaatct ggataaataa attgacggcg gcatcggtaa ttttagactc gttatcatta
      961 aaaactgata cagaaaaaat aaatataatt ttggtgaatg ttttgacatg attaaatcca
     1021 ttttctttat tttcttctga cataatacgt tactcccttt aattattaac gatatgtttc
     1081 gcatttttct ttaaatctgc atgtaacaga atgttaataa ggcatgattg ttgcggcagg
     1141 atctgcaata attaacaatt atccacttca cagagaatgc tatgcagtac ccgattaacg
     1201 agatgttcca gaccctgcaa ggtgagggtt actttaccgg cgtccccgcc atttttattc
     1261 gtttacaggg atgcccggtt ggctgtgcct ggtgcgacac caaacacacc tgggaaaagc
     1321 ttgaggatcg ggaagtctcc cttttcagca ttctggcgaa gaccaaagag agtgataagt
     1381 ggggggcggc gagcagtgaa gatttgctgg ctgtcattgg tcggcaggga tataccgcgc
     1441 ggcatgtggt gattacgggt ggtgagcctt gcattcatga tttgctgcca ctgactgacc
     1501 tgctcgaaaa gaacggtttt agctgccaga tcgaaaccag tggaactcat gaagtacgct
     1561 gcacaccgaa tacctgggtt accgtatcgc caaagctgaa catgcgcggc ggctatgacg
     1621 tgttgtcaca ggcactggag cgagccaacg aaatcaagca tccagtgggg cgcgtacgcg
     1681 atattgaagc actcgatgaa ctattggc
//
LOCUS       DAJTNN010000315         1698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTNN010000315 DAJTNN010000000
VERSION     DAJTNN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.325"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..674)
                     /locus_tag="OO150_004188"
                     /pseudo
     CDS             complement(1..674)
                     /locus_tag="OO150_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            860..1698
                     /locus_tag="OO150_004189"
                     /pseudo
     CDS             860..1698
                     /locus_tag="OO150_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
BASE COUNT          448 a          350 c          385 g          515 t
ORIGIN      
        1 cgaactgtcg ctcaagatga ttcgggatag caacgtgctc atgaccgcca cgcttatacc
       61 ggtgagtcgg ctgctggcaa ctgaccagcc ccagctcttt catgagtctg ccagcaagcc
      121 agcgccccat ctggtagcct ctctgggttg ccattgtggc gatgcttctt gctccggcag
      181 agccgtggct gatgccatgc agttcaagta cctggctgcg taatacagcc cgtctgccgt
      241 ctggtttttc aggacggttt ttccagtatt tgtagctgct gcgatggacc ccgaacacat
      301 ggcagagagt ggccacagga taacgcgccc tgagtttccc gattatcgaa aactgttcag
      361 ggagtctgac atcaagagcg cggtagcctt tttaatattt cattttccat ttcaatacgt
      421 tgtagctttt tcctgagctc acggatttca atttgttccg gggtaatggg ggaggctttt
      481 ggtgtttttc cctgccgctc atcacgtaat tgtttcaccc atcgcgtcat tgtggaaagg
      541 ccgacatcca tagcgctggc tgcatctgcc acggtgtatt tctggtcaac gaccagttga
      601 gcggattcgc gtttaaactc tgcgctgaaa tttctttttt cattatggca cctgtgttgt
      661 tctgaggtga gcatatcacc tctgttcagg tagctaaatt tagtgtgcca cctcaaggtt
      721 cttagtgagc cgtttttaaa agatatgata atatattatt gtttataaat tgttgatgtt
      781 tgatttagta gtatataaag ttcaccacct ttattaggct ttccttttat ctctacttac
      841 tgggtgccag aaactttaaa ttttaaacgg agggggcatt ttgtttaaag caaatcatgt
      901 tccggtatta atgtatcatc atgtcagcca ttgcccagga ctggttacac tttctcccga
      961 gacattccgg aaacaaatga aatggttggc tgaaaacaac tggaaaacac tgtcatcaga
     1021 tgaactggag tttttttatc ggggcggaaa acttccccgt aaaagcgtta tgttgacatt
     1081 tgatgatgga taccttgaca actggtttca ggtatatcct ttattaaact agtttaatct
     1141 caaggcacat atttttttaa ttaccagttt tattggaaat ggtccggttc gtcactctcc
     1201 tggaaaggaa tattctcatc gcgattgcga acatcaaata gcaaccggga atgctgataa
     1261 tgttatgctt cgctggtctg aagtgaacga aatgttgcaa tctggtctgg tcgaatttca
     1321 tgttcataca catactcata cacgttggga taaaaagttc acttcacgag aagaacagtg
     1381 taaacatctt cggcaggact tattgagcgg gagagaatat ttaaaaaaaa tgacaggaaa
     1441 atgcagtaaa catttgtgtt ggccggaagg ttattataac aaggattata ttcagatcgc
     1501 tgaagaactt ggattccatt atttatatac aacagaaaga agaatgaatg ctcctgctaa
     1561 aggaactaca cggataggaa gaataagcac caaggaacgg gaaaattgcg tatggctgaa
     1621 aagacgactt ttttattata caacaccgtt tttttcatcg ttgctggccc ttcataaggg
     1681 gccaagatta ccggatga
//
LOCUS       DAJTNN010000316         1688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTNN010000316 DAJTNN010000000
VERSION     DAJTNN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.326"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            197..991
                     /gene="panB"
                     /locus_tag="OO150_004190"
     CDS             197..991
                     /gene="panB"
                     /locus_tag="OO150_004190"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5518109.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1003..>1688
                     /gene="panC"
                     /locus_tag="OO150_004191"
     CDS             1003..>1688
                     /gene="panC"
                     /locus_tag="OO150_004191"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5518110.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIA"
BASE COUNT          402 a          445 c          454 g          387 t
ORIGIN      
        1 gccacgctac aacaggatgg aactatacca atagaagctg gtgagtttaa agccaccagt
       61 actttccagg taacctaccc ttaataagtc caccgcaccg ccatcatctg gcggtgcggt
      121 aattgataaa tcattgcccc caccaatgtg actgataaaa tgccaggcaa cacgacatca
      181 tttatcagga cacgttatga aaccgaccac cattgcttca cttcagaaat gtaagcagga
      241 taaaaagcgt ttcgcgacca tcaccgctta cgactacagc ttcgcgaaac tatttgctga
      301 tgaaggactt aacgtcatgc tggtaggcga ctcgctgggc atgacggttc agggtcacga
      361 ctccaccctg ccggtgaccg ttgccgatat cgcctaccac actgcctccg tacgtcgtgg
      421 tgcgccaaac tgcctgctgt tggctgacct gccgtttatg gcgtatgcca cgccggaaca
      481 agcctttgaa aacgccgcaa cggttatgcg tgccggtgcc aatatggtca aaattgaggg
      541 cggtgagtgg ctggtcgaaa ccgtaaaaat gctgaccgaa cgtgccgttc ctgtatgtgg
      601 tcacttaggt ttaaccccac agtcagtgaa tattttcggt ggctacaaag ttcaggggcg
      661 cggcgatgaa gcgggcgatc aactgctcag cgatgcatta gccttagaag ccgctggggc
      721 acagctgctg gtgctggaat gcgtgccggt tgaactggca aaacgtatta ccgaagcact
      781 ggcgatcccg gttattggca ttggcgcagg caacgtcact gacgggcaga tcctcgtgat
      841 gcacgacgcc ttcggcatta ccggcggtca cattcctaaa ttcgctaaaa atttcctcgc
      901 cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt
      961 ttatccgggc gaagaacaca gtttccatta aggagtcacg ttgtgttaat tatcgaaacc
     1021 ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg
     1081 gtgcctacta tgggtaatct gcacgatggc catatgaagc tggtcgacga agccaaagcc
     1141 cgcgccgatg tggtcgtcgt cagtattttc gttaacccga tgcagttcga ccgcccggaa
     1201 gatctggcac gttatccacg gaccttgcag gaggactgcg agaagctaaa caaacgtaaa
     1261 gtggatttag ttttcgcccc ttcggtaaaa gagatctacc cgaacggtac tgaaacccat
     1321 acttacgttg acgttcccgg gctttcgacc atgctggaag gcgcgagccg tccggggcat
     1381 tttcgcggcg tttcgactat cgtcagcaag ctgtttaacc tggtccagcc ggacatcgcc
     1441 tgcttcggtg aaaaggactt tcagcaactg gcgctaatcc gcaaaatggt tgccgatatg
     1501 ggcttcgata ttgaaattgt cggcgtcccg attatgcgcg ccaaagatgg ccttgcgctc
     1561 agttcccgta atggttatct gacggcggaa caacgcaaaa ttgcgcccgg tctgtacaaa
     1621 gttttaagtt caattgccga caaattgcag gctggcgagc gggatcttga tgaaattatt
     1681 gccattgc
//
LOCUS       DAJTNN010000317         1684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTNN010000317 DAJTNN010000000
VERSION     DAJTNN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.327"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..173
                     /locus_tag="OO150_004192"
     CDS             <1..173
                     /locus_tag="OO150_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HCR5518111.1"
                     /translation="EVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLRKDIM
                     RRLTQDVCEEIE"
     gene            complement(230..>1684)
                     /gene="ppsA"
                     /locus_tag="OO150_004193"
     CDS             complement(230..>1684)
                     /gene="ppsA"
                     /locus_tag="OO150_004193"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5518112.1"
                     /translation="IEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTL
                     HSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASA
                     IVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLE
                     FSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRA
                     LLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKS
                     NEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVE
                     IMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSI
                     GSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDH
                     EDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          401 a          486 c          406 g          391 t
ORIGIN      
        1 aagaggtggt ggcttttttt agccgtaaac gggtcgcaaa atataaatat cctgaacata
       61 tcgtggtaat cgaaaaacta ccgcgcactg cctcaggtaa aatacaaaag tttttgttaa
      121 gaaaagatat tatgcggcga ttaacgcagg atgtctgtga agagattgaa taagtttcat
      181 cttcggggat cacataaacc cggcgacaaa acgccgccgg gtatttattt tatttcttca
      241 gttcagccag gcttaaccag gtttgcacca cggtgtccgg attcagagac aggctatcga
      301 tcccctcttc catcaaccat gcggcaaaat cttcgtggtc ggacggaccc tgaccgcaaa
      361 tcccgacata tttgccctgt ttcttcgcgg cacggatagc catcgacagc aatgctttca
      421 ccgcatcgtt gcgctcatcg aacagttcag acaccacgcc ggagtcacga tccagaccga
      481 gcgccaactg cgtcatgtcg tttgagccaa ttgagaagcc gtcgaaatat tcgaggaact
      541 gctcggccag caaggcgttg gacgggattt cacacatcat gatgattttc agcccgttct
      601 caccacgctt cagcccctga cgcgccagtt cttcaaccac cgctttcgcc tggtctacgg
      661 tacgcacgaa cgggatcatg atctcaacgt tggtcagccc catgtcgttg cgcacacgtt
      721 tcactgcttc acactccagc gcgaagcagt cgcggaagct gtcggaaaca tagcggcccg
      781 cgccacggaa gccgagcatc gggttctctt catctggctc gtaacgctca ccaccgacca
      841 ggttggcata ttcgttcgat ttaaaatcag agagacggac aatgacgcgc ttcggataaa
      901 acgcggcacc cagcgtcgcg atcccttcag tcagacgacc aacgtaaaat tcacgcggag
      961 aatcaaaacc tttcatcatc tcgcggattt cgttttgcaa ctgcggttcc tgatcgtcaa
     1021 actcaagcag tgcgcgtggg tggacgccaa tcatacggtt gatgataaat tccagacgcg
     1081 caagtcccac gccttcgttc ggcagacagg cgaagtcgaa agctcgatcc gggttaccga
     1141 cgttcatcat cactttcaac ggcagatccg gcatggtttc tacgctggag cttttcacgc
     1201 taaattccag caactccgca tagacgtaac cggtatcacc ttcggcacaa gaaacagtga
     1261 cgttctcacc gtctttcatc cgttctgttg catcaccaca gcccacgacc gccggaatac
     1321 ccagttcacg agcgatgatc gccgcgtgac aggtacgacc gccacggttg gtgactatgg
     1381 cagatgcttt cttcatgatc ggttcccagt ccgggtcggt catgtcagtg accagcacgt
     1441 caccaggttc gatgcggttc atttcgctga tgtcatggat gactttcacc ggacccgcac
     1501 cgatgcgatg accgatagca cggccttcgg cgataatctt accctgtgaa tgcagcgtat
     1561 aacgctccat gacctgaccg cgtgagcgca cggtttccgg acgcgcctgc acaatgaaca
     1621 gtttgccggt gtggccatct ttcgcccact caatatccat cgggcgaccg tagtgtttct
     1681 caat
//
LOCUS       DAJTNN010000318         1657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTNN010000318 DAJTNN010000000
VERSION     DAJTNN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.329"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..256)
                     /locus_tag="OO150_004194"
     CDS             complement(<1..256)
                     /locus_tag="OO150_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518113.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCT"
     gene            197..382
                     /locus_tag="OO150_004195"
     CDS             197..382
                     /locus_tag="OO150_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518114.1"
                     /translation="MPAGRRGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(424..579)
                     /gene="hokD"
                     /locus_tag="OO150_004196"
     CDS             complement(424..579)
                     /gene="hokD"
                     /locus_tag="OO150_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5518115.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1231..1647)
                     /locus_tag="OO150_004197"
     CDS             complement(1231..1647)
                     /locus_tag="OO150_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518116.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
BASE COUNT          408 a          414 c          359 g          476 t
ORIGIN      
        1 atgtgcagga cggacgcggt ggccgttcgg aataagggag cctgacgaag attatccagt
       61 gacgataatc gagactgagg gctttcttaa tctcgtatcc gtgtctgcgg tagcgctgaa
      121 tcagccattc agcctgttct tcggtgcagg ggtcgtgctg ataccagtca gatttgaatg
      181 catgagaacg ccgcccgtgc ctgctggcag gcgcggcaga gttatccgaa ttgtaaaatt
      241 tggtatcgtg cgccatctgt tttctctgct ggcgcagcag gtgccagttg ttcaggctga
      301 cggatggatt gtaaaccaga acgaccagaa aaaacaaaac ccgccgaagc gggttaagtg
      361 cgggtgcgtt gaggatgcct gacacatcag cggtggcgag ggatttctcc cccgccgggt
      421 ctcttactcc tcaggttcgt aagctgtgaa gacagcgacc tccgtctggc cggttcggat
      481 tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc actatgacgg ttaaacagat
      541 gacgataagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt
      601 cggcacgtaa gaggctaacc tacatgtgtt cagcatggat tgagcctcag attaatgtta
      661 agcgtcttgc aggacgcgta gtgttaactg gggcttttct ctatctgcct tttggtgttc
      721 atgcctgaga cagatagcct caagcacccg cagtcattct acttaactaa gatttccctg
      781 caaaccgttt ttgtccggca cagtaaatat ccaactaaac caatagcgtt cgctgtattt
      841 accgccagta ttcaatgcac atgaccgcca tgaacacccc taaaaaaagg gcatttatat
      901 gtccaaacat taatatcaaa acatcaattt tttccatata ccttgctgtg aagatgatgg
      961 gcatacatga tgcgaacaac cagaacgcaa caaacaaaaa ctgcaatgcg tttttcatta
     1021 ttccccctac aatcaatgtg caataacatt taaacacacc tcaatttggc cggacatata
     1081 aatatctaaa ccagaaaaaa tcacttacat agcgttacaa actctttagt ctaaatactc
     1141 atcgtaaaac attccccata cttatcagtc cgttccgcgc caggtagctt attgccttat
     1201 ctggcaacct ataatcaggt ttccgctttt tcagttggct ggtcgtttaa ccgacatagt
     1261 taacccatta atctggttgc cggatgctgg tggattttcg cgttttagtt gttcataaaa
     1321 gtgcacagct ttaaccagtt cttctgatgt aaccgggact ggtggggcag tgaataaggc
     1381 ctgaatttca tagttcggcc tgtcgttaca atcctctttt ttcggtacat atttccagtc
     1441 accaacccac aactccccct gagagtccat aacacctttt ttcacgtagc gatatcgcca
     1501 cgctatcggc tctgcttcca gcgatgcccg tgcaattttg aataactcgc cctctactcg
     1561 cgccatccct gaattggggt ggcatttcgt aatcgctatt tttaatttgg cttcttcgat
     1621 taattgttct tttgttaatt cagtcatttt cattacc
//
LOCUS       DAJTNN010000319         1612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTNN010000319 DAJTNN010000000
VERSION     DAJTNN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.330"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..1566
                     /locus_tag="OO150_004198"
     CDS             <1..1566
                     /locus_tag="OO150_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCR5518117.1"
                     /translation="IRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQ
                     PEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIR
                     AKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDE
                     WENLHALKEKRLNGMPKTTPKSGQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIE
                     RSIIPPLKNYNERLHNTNLFNPKYQKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSA
                     IDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFL
                     FNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCST
                     YQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNE
                     EVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALL
                     LGKTGEPPNYLYL"
BASE COUNT          555 a          304 c          312 g          441 t
ORIGIN      
        1 atcagggaaa atatgtctga gtttgcacgt tgccattatg ggtatataca aatccctcca
       61 gtgacaactt tccgcgccga cggaccagaa actcccgaag aagaaaaagg ttactggttt
      121 catgcttatc aacccgaaga tctttgtacc atccataacc caatgggaga tttgcaggat
      181 tttatcgcat tggttaaaga tgctaaaaaa tttggtatcg atatcattcc tgattataca
      241 tttaacttta tgggaatcgg gggtagtggt aaaaatgacc tggattatcc ctctgctgat
      301 atacgagcga agatcagtaa agatatagaa agcggtatcc ctggctattg gcaaggtcag
      361 gttttgattc cattcattaa agatccagta acaaaagaac gtaaacaaat ccatccagaa
      421 gatatacatc tcactgcaaa agacttcgaa gcaagtaaag ataacatctc taaggatgaa
      481 tgggaaaacc tccatgcatt aaaagaaaag cgtttaaatg gaatgcctaa aacaacaccc
      541 aaaagtggcc aggttattat gttgcaaaat caatacgttc gagaaatgcg aaaatatggc
      601 gtacgaggtt tacgttatga tgcggcaaaa cactcaaaac atgaacaaat agagaggtcg
      661 ataatcccac cgcttaaaaa ttataatgag cggttacaca atactaattt atttaaccca
      721 aaatatcaaa aaaaagccgt tatgaattac atggaatacc tggtaacttg tcagttggat
      781 gaacaacaaa tgtcatcact gctttatgag agagatgatt taagtgccat tgatttttct
      841 ttactcatga aaacgataaa agccttttca tttggtggag atctccaaac ccttgcatca
      901 aaaccgggtt ccacaatctc aagtatccca tcagaaagac ggatattgat taacattaac
      961 cacgattttc ctaacaatgg taatcttttc aatgactttc tatttaacca tcaacaagat
     1021 gaacaattag caatggcata tatggccgct ctcccgttca gcagaccttt agtttactgg
     1081 gatggccaag tattaaaatc aacgactgaa attaaaaatt atgatgggtc cacgcgtgtc
     1141 ggcggtgagg cgtggcttaa taaaggttgc tctacctatc agcagctcta caatgaattc
     1201 cacgcattat atatagataa agcaggaata tggagcgcat ttgagggtgt atttgcaact
     1261 aaaaacgttc tggcctttag tcgtggggat tctgtgaaca ttaatcactc tcctcatgat
     1321 gggctagtta taataaataa aggaaacgaa gaagttgaag gtacctggcc taacaaattg
     1381 caacctggaa tatacaaaaa catggggagt aatagcgtta acattattat taataatacc
     1441 cgaaaaatta tcccccctgg taaagtattt acgcttagag gcggaactct aaatatcaat
     1501 attcctgggc gtagcgctct tcttttaggg aaaacaggag aaccgccgaa ctatctctat
     1561 ttataattta atgttatatc tgccccgata aaacggggca gataatatgt tt
//
LOCUS       DAJTNN010000320         1579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTNN010000320 DAJTNN010000000
VERSION     DAJTNN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.331"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..587
                     /gene="argO"
                     /locus_tag="OO150_004199"
     CDS             <1..587
                     /gene="argO"
                     /locus_tag="OO150_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5518118.1"
                     /translation="PLGPQNAFVMNQGIRRQYHIMIALLCAISDLVLICAGIFGGSAL
                     LMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAEVLKQGRWKIIATMLAVT
                     WLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWFFGLAILAAWLAPRLRTA
                     KSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            680..1420
                     /locus_tag="OO150_004200"
     CDS             680..1420
                     /locus_tag="OO150_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5518119.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
BASE COUNT          373 a          371 c          435 g          400 t
ORIGIN      
        1 tgccgctcgg cccacaaaat gcttttgtga tgaatcaggg cattcgtcgt cagtaccaca
       61 ttatgattgc cttactttgc gctatcagcg atttggtcct gatttgcgcg gggatttttg
      121 gtggtagcgc gttattgatg cagtcgccgt ggttgctggc gctggtcacc tggggcggcg
      181 tagtcttctt gctctggtat ggttttggcg cttttaaaac agcgatgagt agtaatattg
      241 agttagctag tgccgaagtc ctgaagcaag gcagatggaa aattatcgcc accatgttgg
      301 cagtgacctg gctgaatccg catgtttacc tggatacttt tgttgtactg ggcagccttg
      361 gcgggcagct tgacgtcgaa ccaaaacgtt ggtttgcgct cgggacaatt agcgcctctt
      421 tcctgtggtt ctttggtctg gctattctcg cagcctggct ggcaccgcgc ctgcgcacgg
      481 caaaatcaca gcgcattatc aatctggtag tgggatgtgt catgtggttt atagccttgc
      541 agctggcgag agacggtatt gctcatgcac aagccttgtt cagttaggcg ctatctgatg
      601 gaaaaataaa acagaggcgc taagcttgcc tccagaggtc ctgaattttg ggcaagtcaa
      661 ttaacacgga gagactaacg tgaagttcaa agttatcgcc ctagcggcat taatgggtat
      721 tagcgggatg gcagcgcagg ctaacgaatt gccggatgga ccgcatattg tcacctccgg
      781 tacggcaagt gtggatgcgg tgccagacat tgccactctt gcgattgaag ttaacgtggc
      841 cgcgaaggat gccgctacag ccaagaaaca ggcagatgag cgcgtcgcac aatacatttc
      901 cttccttgaa ctcaatcaga tcgcgaaaaa agatatcagc tcagcgaatt tacgcaccca
      961 gccagattat gattatcagg atggtaaaag tatcctgaaa ggctaccgcg ctgtgagaac
     1021 ggtggaagtc acgctccgtc agttagacaa actgaattcc ttgctggatg gcgcgctgaa
     1081 ggcgggtctt aacgaaattc gttcagtgtc gctgggcgtg gcgcagccgg atgcctataa
     1141 agacaaagcg cgtaaggcag cgattgataa cgcgattcat caggcgcagg aactggcgaa
     1201 cggctttcat cgtaaactgg ggccggtata tagcgtgcgc taccatgttt ccaactatca
     1261 gcccagccca atggtgcgga tgatgaaagc cgatgccgcg ccggtgtctg cccaggaaac
     1321 ttacgagcag gccgctattc agtttgatga tcaggtcgat gtggtcttcc agttagaacc
     1381 tgtggatcaa caacccgcta aaacacctgc agcacaataa tacttgtagc ccgacgtatt
     1441 cgtcgggcta atgcctttac ccgatattgc ctcctttttc tgtaattttc tgtaaaacca
     1501 tgacgtaatc acctatcttt gctcgcggtt tcatcaagga taggctgact gaaacggtta
     1561 agcggacaag gatgcgggg
//
LOCUS       DAJTNN010000321         1557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTNN010000321 DAJTNN010000000
VERSION     DAJTNN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.332"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..627
                     /locus_tag="OO150_004201"
     CDS             <1..627
                     /locus_tag="OO150_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5518120.1"
                     /translation="DQKSVNVRLENKGNNPLLVQSWLDTSDDNAEPGSITVPFTATPP
                     VSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRT
                     RIKLFYRPDGLKGNPSEAPLALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKR
                     YPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGTIEGNASL"
     gene            662..1557
                     /locus_tag="OO150_004202"
                     /pseudo
     CDS             662..1557
                     /locus_tag="OO150_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          456 a          338 c          339 g          424 t
ORIGIN      
        1 gatcaaaaaa gtgtcaacgt acgtctggaa aataaaggga ataacccgtt gcttgtccag
       61 agttggttag atactagcga tgacaacgct gagcctggca gtattacagt cccttttact
      121 gccacgccgc cagtatcgcg tattgatgcc aaacgtgggc aaacaatcaa attaatgtac
      181 acagccagca cctcactgcc taaagacaga gagagcgtgt tctggtttaa cgtactggaa
      241 gttccaccaa aaccagatgc agaaaaggtc gcgaatcaaa gtctgctgca actggcattt
      301 cgcacacgta taaaactttt ctatcgcccg gatggattga agggaaatcc ctctgaagcc
      361 ccgttagccc tgaagtggtt ctggtcaggt tcagaaggta aggcgtcatt gcgagtgacc
      421 aatccaaccc cttactacgt ctcttttagc agtggtgatt tagaagctag cggtaaacgc
      481 tatccgattg atgtgaaaat gattgcacca tttagtgatg aggtcatgaa agtcactggc
      541 cttaatggca aagcaagttc tgcaaaagta catttttacg ccattaatga ttttggtggc
      601 accattgaag gtaatgccag tctgtaatca ggcaaggata taattccgca ggaagcataa
      661 cgtgactata aaaccaacta aaaattatca taatcatctg acccgtatcg ctacgttttg
      721 cgcgctgctg tattgcaata gtgctttctg tgctgaactt gttgaatatg accatacctt
      781 cctgatgggg cagaatgcct caaatattga tctcagccgg tatagtgaag gtaaccctgc
      841 tataccgggt atgtacgacg tcagtgttta tgtcaacgat caacctatta ttaaccaaag
      901 cattacattt attgaaattg aaggaaaaaa aacgcacaag cctgtatcac acttaagaat
      961 ttattgcagt ttcatataaa ttctcccgat ataaataacg aaaaagccgt tctgcttgcc
     1021 agggatgaaa cactcggcaa ttgccttaat ttgacggaaa ttatccctca ggcttctgtt
     1081 cgttatgacg ttaacgagca acgtctggat atagacgttc ctcaggcatg ggtaatgaaa
     1141 aattaccaaa actatgttga tccatcgtta tgggaaaatg gcattaatgc ggccatgttg
     1201 tcatacaacc tcaacggata tcatagtgaa actcctggtc gaagaaatga cagcatttat
     1261 gctgctttta acggtgggat gaatttaggt gcatggcgac tgcgtgcctc aggcaactac
     1321 aactggatga ccgattctgg cagtaattat gattttaaga atcggtatat acagcgtgat
     1381 atcgcctcgc tgcgttctca actcattctt ggtgagtctt atacaacagg cgaaaccttt
     1441 gattctgtca gtatccgcgg cattcgttta tacagcgaca gccgcatgtt gcctccgaca
     1501 ttagcgagtt ttgcgcctat cattcatggc gttgctaata ccaacgccaa agtcact
//
LOCUS       DAJTNN010000322         1527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTNN010000322 DAJTNN010000000
VERSION     DAJTNN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.333"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..1386)
                     /locus_tag="OO150_004203"
                     /pseudo
     CDS             complement(1..1386)
                     /locus_tag="OO150_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          525 a          263 c          224 g          515 t
ORIGIN      
        1 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
       61 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
      121 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
      181 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
      241 tatttttatc aatcagccct ttattcatat taaacctgtt aataaaatgg atcatctgtt
      301 gggtatcgaa atccaccttg ctgaatcgat gttgacgcat aaaatcatcg aacttgttat
      361 cttcaccaga atggatataa ctgcgtataa ttggatcttt tatataagga caatggctaa
      421 atgttttctt catattctca ataaagtttt tattaacaat attaagaatt tttaattgca
      481 taaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
      541 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
      601 aatcaatagt taattccaat tgttctgtaa tgcaatcttt aaaattccct gtcgacattc
      661 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
      721 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
      781 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
      841 tatcaagaat atcttggtta aaatcaacat attcgccact cgttttgaga aaaatcacat
      901 tgtgtaaacg tcgacattta aatatgacat tatcaactgt tgccttagta aaattgcaat
      961 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
     1021 tagaaataac gtcaccttta aaatgacaat ttgttacatc aaaatcagac aggtcgatat
     1081 acccctgcga atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
     1141 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
     1201 gattttttcg ttccgttgtt ttatatttaa aaatatatta ctgatataaa taaagttatc
     1261 tctgtttaaa cctgtcaccc cattgaaatt acgtagtaaa tcaagatcta ctatattatt
     1321 tccttgaacc acatcatgtt tcatcaaaga gttattgcca ccagaatttg tgatatgacc
     1381 tatcataaaa cctctgtaat acagaaataa aataacctgg aaaagtagcc tgagcgtcca
     1441 gacactaccg gcgaaaggaa aagaatctct gataaggcat tgagataatg gacattctta
     1501 gtgaagaaga agtgaagtac tataaaa
//
LOCUS       DAJTNN010000323         1495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTNN010000323 DAJTNN010000000
VERSION     DAJTNN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.334"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO150_004204"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO150_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5518121.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
     gene            1117..1495
                     /locus_tag="OO150_004205"
                     /pseudo
     CDS             1117..1495
                     /locus_tag="OO150_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          451 a          267 c          292 g          485 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcagaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaaga gagctaaata gctgcgcgga atagtaggct gtgtccctta atcatctgag
     1081 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     1141 gaggcatgga ccatcatcaa gcccctgtta cctcctgaac ccgccacacc acgggccgga
     1201 cgcccatggg ctgagcaccg taaaatcatc aatggcatgt tctgggtgtt atgttccggt
     1261 gcaccatggc gcgatttacc cgaacgatat ggggcatgga aaactgttta taaccgcttt
     1321 aaccgatggt caaagtcagg cgtgattaat attattttca acaggttgct ttcgctactt
     1381 gatgcaaacg gctttattga ctggtctgcc accgcgctgg atggcagtaa tatccgggcg
     1441 ctgaaatgtg ccgccggtgc tcaaaaaaac atcccgatat cgacggagat aatgg
//
LOCUS       DAJTNN010000324         1477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTNN010000324 DAJTNN010000000
VERSION     DAJTNN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.335"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..732)
                     /gene="pdeC"
                     /locus_tag="OO150_004206"
     CDS             complement(<1..732)
                     /gene="pdeC"
                     /locus_tag="OO150_004206"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5518122.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFSSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLI"
     gene            1162..1443
                     /locus_tag="OO150_004207"
     CDS             1162..1443
                     /locus_tag="OO150_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5518123.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          427 a          324 c          330 g          396 t
ORIGIN      
        1 aataagatga ttataaaaat gggttataag acgttgatat gaagtcgcaa caatggctgc
       61 aattgggcgt tttgtcgaat aaactgtcgc atataaatag ccatttcttt gtacggttaa
      121 atccttcaaa tgaatcagcg gagaaaacgt tgctgccgag gcctctttgc ttaacgagaa
      181 aaaggttttc gtcaccgtat catacacacc ccattgcaat gtcggatcat cagacatcac
      241 ttcactccag aagagagggt tgataaccgc cacataattt ccccgctgca tataggtcat
      301 tttatagcca gaggaaaaag gcgtatcgcg gtaataatag atagaaacgt taggttcacg
      361 cttataatcg gccggtgcaa tcgtatagcc gtttacaggc gctatcagcg atgagcataa
      421 aaaatggtta tcacgggcat agatcaattc attaatataa agatagccac gaataatatt
      481 caacattcgc ttttgatggg ctggagtgca aacttgcccc tgatagcgtt cagctgcatc
      541 gctcactaaa tctgcctggc gaatgaccag ctcggattta tccagtgcca gttgagcaaa
      601 ggttcggagc tgattattca cttctgattt tgcccaaagg aaggcaatcc atagcgaaag
      661 aatgatggga aagagaacta aaaagataat gcccggcaac gccagtaatt ggtgtcgtgc
      721 acgatgactc attgctatca atttcctgcc tgtttatacc cggccattgt aaggtgaaaa
      781 ccctgggtac cgacgtcttt agataaatct tacggtataa atagcacttt tgcctgattt
      841 gaaattgata cctttctaat cgtcacctgg atgaaaataa ttcattgata ttcaaaatta
      901 atttcctaca ggaatgcaga ggcggcggga agatatgccc ttaatgccac tgaaaaacca
      961 cataatctgt acagcaataa aagtcacggc ctaatatggc taacaaacaa tcaattgtga
     1021 tatatttcac aaaattaatg aaacaaacag agtgtttcat ttttgtgttc tgcaagcccc
     1081 gcgatagcca gagtgtacca cgcctcccgt gaacaacgcc gcgctgtcca gggcatcggc
     1141 tcttttcaga ggaattgttt gatggctacc ctcaccactg gcgtggttct tcttcgctgg
     1201 caacttctta gtgccgtaat gatgtttctg gccagcacac tcaacatccg ttttcgtcgg
     1261 tctgattatg tcgggcttgc ggtgatcagc agcggtctgg gcgttgtttc cgcctgttgg
     1321 ttcgcaatgg ggttgcttgg catcacaatg gcggacatca ccgctatctg gcacaacatc
     1381 gagtcggtga tgatagaaga gatgaatcag acaccgccac aatggccaat gattttgacc
     1441 tgatacgtag aagcctccat aacggagtaa tgccgat
//
LOCUS       DAJTNN010000325         1477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTNN010000325 DAJTNN010000000
VERSION     DAJTNN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.336"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>1477
                     /gene="pdeB"
                     /locus_tag="OO150_004208"
     CDS             <1..>1477
                     /gene="pdeB"
                     /locus_tag="OO150_004208"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5518124.1"
                     /translation="LSIWLAHQQVETSFIEELDTYSSRVAIRANKVATQGKDALQELE
                     RWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNVPQCSSLEHESPPDTFPEPGKISK
                     DGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGNAHNV
                     VITSSDEIAQGIITRLQKTPGEHIENNGIIYDILPFPEMNISIITWASTKMLQKGWHR
                     QVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDAIENRDICVHYQPIVSLANGKI
                     VGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMGDWLRQHPQQH
                     ISINLESTVLTSEKIPQLLREMINHYQVNPRQIALELTEREFADPKTSAPIISRYREA
                     GHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVDALEYKNVTPHIIEMAKTLKLKMV
                     AEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDFLRWAEQ"
BASE COUNT          395 a          347 c          361 g          374 t
ORIGIN      
        1 gcttaagcat ctggctggcc catcagcagg tagaaacatc gtttattgaa gagctggata
       61 cctattcctc ccgcgtcgct attcgggcca ataaggtggc gacacaaggg aaagatgcgc
      121 tgcaagagct ggaaagatgg caaggcgctg cctgtagcga agcccatctc atggaaatgc
      181 gtcgggtatc ttacagttat cgctatattc aggaagtggt ttatatcgat aacaacgttc
      241 cccagtgttc gtctctggag catgaaagtc cgcccgatac cttccccgag ccaggtaaaa
      301 tttcgaaaga tggttatcgt gtctggttaa catcgcataa cgatttaggc attatccgtt
      361 acatggtcgc catgggaacg gcacattatg tcgtcatgat cgaccccgct tcctttattg
      421 atgtcattcc ctatagctca tggcaaattg atgccgccat tattggcaat gcccataacg
      481 ttgtcataac cagcagcgat gaaattgctc agggaattat taccaggcta caaaaaacac
      541 ccggtgagca tatcgaaaat aatggaatca tttacgatat cctgcccttc ccggagatga
      601 atatttcgat catcacctgg gcttcaacga aaatgttgca gaaaggctgg catcggcaag
      661 tctttatttg gttaccgctc gggttggtga ttggcctgct ggcagcgatg tttgtgctgc
      721 gtattttgcg ccgtatccag tcaccgcatc atcggctgca ggatgctatc gaaaatcgtg
      781 atatttgcgt gcactatcag ccgattgtct ccttagccaa tggcaaaatt gtcggtgctg
      841 aggcactggc gcgctggccg caaacagacg gtagttggtt gtcaccagat agttttattc
      901 cgctggcaca gcaaacaggc ctttctgagc cattgacgct actgattata agaagcgtct
      961 ttgaagatat gggcgactgg ctgcgtcagc atccacagca gcatatttcg atcaatcttg
     1021 aatccaccgt gctcacctcg gaaaaaatcc cgcaattgct gcgtgaaatg atcaatcact
     1081 atcaggttaa tcccagacag atcgcgcttg aactcactga acgcgagttt gccgatccga
     1141 aaaccagcgc cccgataatt tctcgctacc gggaggcggg ccatgaaatt tatcttgatg
     1201 attttggtac gggatattca agtttaagtt atttacagga tctggatgtc gacattctga
     1261 agatcgataa atctttcgtt gatgcgctgg aatataaaaa tgtcacgccg catatcatcg
     1321 aaatggcaaa aacactgaaa ctgaaaatgg tagcggaggg aatcgaaacc agtaaacaag
     1381 aagagtggct acgccagcat ggcgtgcact acggtcaggg ctggctctac agcaaggcat
     1441 taccgaaaga agatttctta cgctgggccg agcaaca
//
LOCUS       DAJTNN010000326         1466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTNN010000326 DAJTNN010000000
VERSION     DAJTNN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.337"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..839)
                     /locus_tag="OO150_004209"
     CDS             complement(<1..839)
                     /locus_tag="OO150_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5518125.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPD"
     gene            complement(1203..1319)
                     /locus_tag="OO150_004210"
     CDS             complement(1203..1319)
                     /locus_tag="OO150_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5518126.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
BASE COUNT          433 a          336 c          262 g          435 t
ORIGIN      
        1 tcatccggta atcttggccc cttatgaaag gccagcaacg atgaaaaaaa cggtgttgta
       61 taataaaaaa gtcgtctttt cagccatgcg caactttccc gttctttggt gcttattctt
      121 cctatccgtg tagttccttt agcaggagca ttcattcttc tttctgttgt atataaataa
      181 tagaatccaa gttcttcagc gacctgaata taatccttgt tataataacc ttccggccaa
      241 cacaaatgtt tactgcattt tcctgtcatt tctttcaaat attctctccc actcaacaag
      301 tcctgccgaa gatgtttaca ctgttcttct cgtgaactga actttttatc ccagcgtgta
      361 tgagtatgcg tatgaacatg aaattcgacc agaccagatt gcaacatttc gtttacttca
      421 gaccagcgaa gcataacatt atcagcattc cctgtcgcta tttgatgttc gcaatcgcga
      481 tgagaatatt cctttccagg agagtgacga accggaccat tcccaataaa accggtaatt
      541 aaaaaaatat gtgctttgag attaaactct tttaataaag gatatacctg aaaccagttg
      601 tcaaggtatc catcatcaaa tgtcaacata acgcttttcc gggggagttt tccgccccga
      661 taaaaaaact ccagttcatc tgatgacagt gttttccagt tattttcagc caaccatttc
      721 atttgcttcc ggaatgtcac gggagaaagt gtaaccagtc cggggcaatg actgacatga
      781 tgatacatta ataccggaac atgattggct ttgaacaaaa tgcccccttc gtttaacatt
      841 taaagtttct ggcacctagt aagcagagac aaaaggaaaa cccaataaag ctggtgcatt
      901 ttatttccag atactggatg aataacatag caaacccatg gcatacttga tatattttac
      961 taaattaaac atcaacaatt cataaacaat gatatattat catatctttt aaaaacgact
     1021 cactaagaac cttgaggtgg cacactgaat ttggctacct gaacagaggt gatatgttca
     1081 cctcagaaca acacaggtgc tccaatgaaa aaaaggaatt tcagcgcaga gtttatttac
     1141 ggggtgacgc tttcattaac ctgtacacga acttctattc cggcatcatg acaggcctgc
     1201 agctactgcg ccatttccag cggatcgccc tcccggcgta ccaccccacc ttctttattc
     1261 cataactgca gagcattttt ctgattgttc catctgattt cggggggtat tccgggcaag
     1321 gttgttcagt aaggcattcc ttcgtaaaat cagcatgttt tttgcacgac ggaaaccggc
     1381 agccatgaaa atctgtcttt atcatactct gaatcctgac acgatcccgg gttataaaaa
     1441 atttgcacag gctattgcca ccgata
//
LOCUS       DAJTNN010000327         1418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTNN010000327 DAJTNN010000000
VERSION     DAJTNN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.338"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..1418)
                     /locus_tag="OO150_004211"
                     /pseudo
     CDS             complement(1..1418)
                     /locus_tag="OO150_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          356 a          364 c          310 g          388 t
ORIGIN      
        1 aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc aatatatccc
       61 gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt ccagtcccat
      121 tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc tggataagca
      181 ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt gatggtcagt
      241 gcttgtttcg ctactcctgc caatttttgg ccttcatctg atgagtccag gccagcaaag
      301 acgccacctc tttaccatcc catgcgcggg atttagcgta agccgtttgt gtcgtctggt
      361 cagtttggag aatgttgcca tgcaccgtac gccagacagt aaaggtctct gcctgaccat
      421 ttttcaccgt aatggtttcc tcacggctta acattttcac ccacttacaa ttatgtaagt
      481 agtagcctgg tttctctgcc gacagccgtt cagcaaaaat atcgacatca tcgccgaaac
      541 ctgccgttga tccccaggaa atcacaccat tatgaccaaa aaccagccca ggataggcaa
      601 atggtgtatt gccagtgaca tcataaccag caccgtgcag accaatacca taagtatacg
      661 caggcgcata ccagccaaac tgcggaccat ttaccatgat tgctttcgca tcctgggctt
      721 tgcttttgcc gatcacccac atattgctgg tcgttggata ccccgccaga ccattggcac
      781 caccctgtgc aaattgtgca gcaatagttt cccggttctt ccctgctgtt aacgccagca
      841 gtgcgccatc cgcccctttt gctggtcggt caagcattgg tgcaggtaaa tcgtagcgtg
      901 gcaacagagc tgctgtttgc gagttttgct gattaaattt aagtgggtag ctactctctt
      961 gtacggcaat agtggttggc gctgatgggt ttaccagcca tttcaactga ttaaataccg
     1021 ccatgccttg tgatacacca tatttatctt ttaaagccgt tagcagtgcc agattatcaa
     1081 tttcgctagt gctatcagag aagcggtttg ccatggtgcc cacaaatatc atcgcgacat
     1141 caaacggttc ccagcgctta ggagtaaagc caaatgtatt aaactgtttt ggtaagagcg
     1201 tctctggatt ggtatttacc ttatcaatcc aggcattcat tccatcagcg tagccttgca
     1261 gaatggacat atcctctggg gaaagggcat caatttgcgc ccggatagca tccggccagt
     1321 agttacgacg gatatcttta tcaaatttca caaaatcttt gccaagcact tccgcgacag
     1381 tcccttgagt actgcgacgt gccatttcca tctgaaaa
//
LOCUS       DAJTNN010000328         1409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTNN010000328 DAJTNN010000000
VERSION     DAJTNN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.339"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(340..606)
                     /locus_tag="OO150_004212"
     CDS             complement(340..606)
                     /locus_tag="OO150_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518127.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPATNRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(788..1186)
                     /locus_tag="OO150_004213"
                     /pseudo
     CDS             complement(788..1186)
                     /locus_tag="OO150_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          362 a          286 c          294 g          467 t
ORIGIN      
        1 aaagccgtct atgggaagga gtctgctgtt attccagaga atggtaattc agtcatatat
       61 ttctccatct atgacttgtc aataccatcc tgatatttgt gggtcagcaa tatattctac
      121 taaaaggtat ttaaggtcat tgcgtccctg cactcgaata atggtgaaga aagcatgatg
      181 aatatatcac gataacgttc agtatgtatc gaggatagat ggagttactc ctctctgaaa
      241 tagaattact gctctacttt ttctgtttcc agaaattatt agtaacaaac cctgatatat
      301 tcttttgaaa atggagctta aacgagtctg aaattaactt cagatattag aatgccgttg
      361 gctttgtttt tcaacaattc ctacttcatt tatctttgtc aaccattgca tttctgtagc
      421 agtgaatttc aacgttttcg tccggttggt agcaggtatt tgtaattcgt atatagctgc
      481 ttttttattg aaaacagtat atccatctgt agtaacagta gaagataata atacaggatt
      541 tgacacttca aggatgatat tttcttgttt ttgtggaagg caagggaata tagtttgtgt
      601 aagcatattg cttccttatg ttatatttta gtaaagaatt tattttttac attatagtcg
      661 gctaaatgaa gtttactgta aaaaaacgat acttttttcg taagcgatga gatgtggagc
      721 cagaaaaatg gctgcgttac gtcattgaac tgtccttgac ccacgatatt cagacagtcg
      781 ttgtatctta agttgacatt acccgctgac gtcgatattc ccttggtgag agataaccca
      841 acgcactatg cggatggttt tcattgtaat gctcgatcgc cactgcaagg ttatgcaatg
      901 ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt catcgttttc acgaaccgtt
      961 ctgctattcc attactctga gggctactga ctgccgtcgt gcagggctcc agattcaact
     1021 ctctggcgaa ctgccgtgtt tcatgagccg tataagccga accgttatcg gccactggat
     1081 tggccgttcc ggtcctttat cgccatatcg ctgacatgac atcctgcacc gttgctttgt
     1141 cataatctcc cgtgcctgct gcccagtcta tggcttcacg atcgcaatat ggaacgtaac
     1201 gctgtcatcg ggagaatcgt caatgcggca ttcggttaac gctgatatga ctttccgtgt
     1261 caaaccggaa tctctaattt tgacgatttt ctttttaggt ttaattccct gagggcgacg
     1321 tagcagtaaa tccgacggtg aatcacgctg atattcgcgg gttggatacc gcattacaga
     1381 ggtccgtcat atgagcctgt cactttctt
//
LOCUS       DAJTNN010000329         1399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTNN010000329 DAJTNN010000000
VERSION     DAJTNN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.340"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO150_004214"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO150_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5518128.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTNV"
     gene            complement(1183..>1399)
                     /locus_tag="OO150_004215"
     CDS             complement(1183..>1399)
                     /locus_tag="OO150_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5518129.1"
                     /translation="AEEQKYEMLENEYSQRVADRLKASGLSGDTDAEREAGAQVMRET
                     EQQIYRQLTDEVLALRLSENGSRLHHS"
BASE COUNT          378 a          286 c          298 g          437 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgtcaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagcctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtcg
     1201 tgagccgttt tcagacaatc gcagggccag tacctcgtca gtcaactgac ggtaaatctg
     1261 ctgttcagtc tcacgcatca cctgtgcacc ggcttccctc tccgcatccg tatcaccgct
     1321 cagacctgat gctttcagcc ggtcagccac cctctgagag tactcattct ccagcatctc
     1381 atacttctgc tcttctgcc
//
LOCUS       DAJTNN010000330         1381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTNN010000330 DAJTNN010000000
VERSION     DAJTNN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.341"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..166
                     /locus_tag="OO150_004216"
                     /pseudo
     CDS             1..166
                     /locus_tag="OO150_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(183..362)
                     /locus_tag="OO150_004217"
                     /pseudo
     CDS             complement(183..362)
                     /locus_tag="OO150_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            693..>1381
                     /gene="ipaH7.8"
                     /locus_tag="OO150_004218"
     CDS             693..>1381
                     /gene="ipaH7.8"
                     /locus_tag="OO150_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5518130.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
BASE COUNT          454 a          290 c          206 g          431 t
ORIGIN      
        1 aggagaaaca cctgaccggc aaaatcttca cgcaacgtat gaacgtaaca acctgaccct
       61 ccgcccccgc atcaagcggc tggcacgtaa aacaatctgc ttctcgcgtt ccgtggaaat
      121 ccacgaaaaa gtcatcggtt ccttcattga aaagcacata ttctaatgat tggaagtact
      181 acccggcaaa actcattcag tgcattccag tgattcagga tatagttgaa cgcctttacc
      241 atttcagcat ggtttgacaa agttttcctc tggagcttta tccagtcata caaggacttc
      301 attcgcggca cacatttctc ctgccttacc gcaagccgtt tttctgccgg cctccctcat
      361 attaaatgtg tatcgttttt ttacagtaat tttaatttat cattctataa taggaataaa
      421 tgagcttggc taaaatatga tgtaaaacaa atatacttac tcggattaca tttccttgct
      481 cactggaatg ttgaattcag tgttattatt gactgcagtc acaataaaaa aaaagaattc
      541 catagaaaac ctcaacaaat aaattacaac taacttctgt tatgtgtaaa atggaaacta
      601 ttaaaactta atatcgaaaa tggtaagtga aatttgtata aatatacaat tttaaatatt
      661 tattctcaca aatataaggt tgccctagca ttatgttctc tgtaaataat acacactcat
      721 cagtttcttg ctccccctct attaactcaa actcaaccag taatgaacat tatctgagaa
      781 tcctgactga atgggaaaag aactcttctc ccggggaaga gcgaggcatt gcttttaaca
      841 gactctccca gtgctttcag aatcaagaag cagtattaaa tttatcagac ctaaatttga
      901 cgtctcttcc cgaattacca aagcatattt ctgctttgat tgtagaaaat aataaattaa
      961 catcattgcc aaagctgcct gcatttctca aagaacttaa tgctgataat aacaggcttt
     1021 ctgtgatacc agaacttcct gagtcattaa caactttaag tgttcgttct aatcaactgg
     1081 aaaaccttcc tgttttgcca aaccatttaa catcattatt tgttgaaaat aacaggctat
     1141 ataatttacc ggctcttccc gaaaaattga aatttttaca tgtttattat aacaggctga
     1201 caacattacc cgacttaccg gataaactgg aaattctctg tgctcagcgc aataatctgg
     1261 ttacttttcc tcaattttct gatagaaaca atatcagaca aaaggaatat tattttcatt
     1321 ttaatcagat aaccactctt ccggagagtt tttcacaatt agattcaagt tacaggatta
     1381 a
//
LOCUS       DAJTNN010000331         1355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTNN010000331 DAJTNN010000000
VERSION     DAJTNN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.342"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            21..542
                     /locus_tag="OO150_004219"
     CDS             21..542
                     /locus_tag="OO150_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5518131.1"
                     /translation="MDVYANSQRVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRSVVAELGDDSGWHFFSQRNTDNSITFAVNGQVIPLNYGNFDARYKYRT
                     EGVQDVRYGHEMYYSPGSNTVSWRFRAPSGHGLSGIAISDTGRDSADNVNGVYYRPLQ
                     KLINGTWYNVASV"
     gene            552..1091
                     /locus_tag="OO150_004220"
     CDS             552..1091
                     /locus_tag="OO150_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5518132.1"
                     /translation="MLKNITAGNPKTVEQYQLTKQYDVTWLFSKDGKNWYEELKNFAS
                     DTIKITYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFD
                     YRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSVI
                     TDKTSYNAIEWPASPEISS"
     gene            1115..>1355
                     /locus_tag="OO150_004221"
     CDS             1115..>1355
                     /locus_tag="OO150_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5518133.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKPGWHNEYNVGDIAVYED"
BASE COUNT          377 a          295 c          352 g          331 t
ORIGIN      
        1 aacagaatgg agacggtatt ctggatgttt atgctaatag tcagcgtgta ttccgctttc
       61 agaatggagt ggctattgct tttaagaata ttcaggccgg aactgccaga aaattcacgt
      121 tatccagcgc caacaactcc acgaaaaatg cagcgtttta tttgtggggt aatccatcca
      181 ggtctgttgt tgcagagctt ggcgatgatt caggctggca ttttttcagc cagaggaata
      241 cagataacag catcacgttc gccgtaaacg gacaggtaat tccgttaaat tacggaaact
      301 tcgatgcccg ctataaatat cgaacagagg gggtacagga tgtacggtat ggccatgaaa
      361 tgtattacag ccccggcagt aacaccgttt cgtggagatt tcgcgcacct tcgggacacg
      421 ggctgtcagg gatagcgata tcggataccg gccgtgactc agcggataac gtcaacggcg
      481 tgtattaccg cccgcttcag aaactgatta atggcacctg gtataacgtg gcgagtgttt
      541 aacaatgttg catattaaaa aatattactg cgggcaatcc gaaaaccgtg gaacaatatc
      601 agctgacaaa acaatatgat gtcacctggc ttttttcgaa agacggaaaa aactggtatg
      661 aagagctgaa gaatttcgcc agcgacacaa ttaaaataac ttacaccgga gacggtcgcg
      721 tggtgtgggt cggtaaggat gtgactggca ttgaaccccg taacgccagt gttattgaag
      781 ttcctgatat taccgccaac cgccgcatta ccgcgccggg ttactggttt taccgcaatg
      841 atgaatttgt ctttgactac agactcaaag cggaagatga gcgtgatgcc cttctggctc
      901 aggtcagtgc ccggacaggg gaatgggaag aagacctgct gctggggcta atcagcgacg
      961 aagaccggga aaaactgaaa gcgtaccgca tttacgcgaa atcgctgcag gcgatggatt
     1021 tcagtgtcat cactgataaa acctcataca acgccattga atggcccgcc tctccggaaa
     1081 tttcttcctg atttaattta tcgcgagaaa aactatgtct gtagtgatat caggtgcgct
     1141 gactgatggc gcaggtatcc ccatgtccgg ataccatatt attctgaaat cccgggtaaa
     1201 caccccggaa gtggtgatgc acactgttgc tgatgtgatg acaggaaacg atggtgaata
     1261 ctgtttccat gcgcggactg gaaaatatgg tgtgtatctg aaaccgggct ggcacaacga
     1321 gtacaacgtt ggcgacattg ctgtatatga ggact
//
LOCUS       DAJTNN010000332         1348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTNN010000332 DAJTNN010000000
VERSION     DAJTNN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.343"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..330
                     /gene="fliH"
                     /locus_tag="OO150_004222"
     CDS             <1..330
                     /gene="fliH"
                     /locus_tag="OO150_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5518134.1"
                     /translation="LMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQL
                     RVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQELCR
                     LAAPGVV"
     gene            330..1348
                     /locus_tag="OO150_004223"
                     /pseudo
     CDS             330..1348
                     /locus_tag="OO150_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
BASE COUNT          277 a          375 c          405 g          291 t
ORIGIN      
        1 ctgatgcaga tggcgctgga ggcagcacgt caggtcatcg gtcagacgcc aacagtggat
       61 aactcggcac tcatcaaaca gatccaacag ttgttgcagc aagaaccgtt attcagcggt
      121 aaaccacagc tgcgcgtgca cccggatgat ctgcaacgtg tggatgatat gctcggcgct
      181 accttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccatcc tggcggctgt
      241 aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg gcaagaactc
      301 tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg cctgactcgc tggctaacca
      361 cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc tacgggcgat
      421 taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg ctcggcgcaa
      481 cctgtgtgat tgagcgccag aacggcactg aaacgcacga agtagaaagt gaagtcgttg
      541 gctttaacgg tcaacggctg tttttaatgc cgctggagga agtcgaaggt gtcctgcccg
      601 gcgcgcgtgt ttatgccaaa aacatttcgg cagaagggct gcaaagcggc aaacagttac
      661 cgctcggtcc ggcgttatta ggtcgtgttc tggacggcag cggtaaaccg ctcgatggcc
      721 tcccccgata cgacggaaac cggtgcgctg attaccccgc catttaaccc gttgcaacgt
      781 acaccgattg aacatgtgct ggacaccggc gtgcgcccaa tcaatgccct gcttaccgtt
      841 gggcgtgggc agcgtatggg gctgtttgcc gggtccggcg ttggtaaaag tgtgctgctg
      901 gggatgatgg cacgttacac ccgcgccgat gtcattgtcg tgggtttgat tggtgaacgc
      961 ggacgcgaag taaaagattt tattgagaat atcctcggtg ccgaagggcg tgcacgctcc
     1021 gtggtgattg ccgctccggc ggatgtttct cctctcctgc gaatgcaggg tgccgcctat
     1081 gccacgcgca ttgccgaaga ttttcgcgat cgtggtcagc atgtgttgct gattatggac
     1141 tccctcaccc gctacgcgat ggcccagcgt gaaattgcgc tggcgattgg cgaaccaccc
     1201 gccactaaag gttatccccc gtcggtgttt gccaaattac cggcactggt cgagcgtgcc
     1261 ggaaatggca ttagcggcgg cggatcgatt accgcgtttt ataccgtgct cactgaaggc
     1321 gatgaccagc aggaccccat tgcagact
//
LOCUS       DAJTNN010000333         1330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTNN010000333 DAJTNN010000000
VERSION     DAJTNN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.344"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>1330
                     /locus_tag="OO150_004224"
     CDS             <1..>1330
                     /locus_tag="OO150_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR5518135.1"
                     /translation="KGGKDGITMTFIGPSPSVQDKSASTDNTNINGEDMTEIEESMLL
                     PVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVTALAISHKTSLLDSL
                     EIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYTDRGLLTKEWMKGNRVSRI
                     TRTASGANAGGGNKTDRNPNLVHTLDTLDVEIAAATLPMDFNIYEIPGSVYRRAKEVV
                     LKKESPFKEWSAALRAPPGILDYSRAAIFALIRSAHPEFYHYPGRLQGYINAYLTETD
                     HENPSKETLTATRHTPEKDILEEINREVVTERETEEEKPQPSDAMAGEQATTETMEPD
                     TTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIMQAANISQPDADKLLAVSRGEFVEG
                     ISDPNDPKWVKGIQTRDSVNQNQHESERNDQKAEQNSPNALQNEPETKQS"
BASE COUNT          403 a          344 c          313 g          270 t
ORIGIN      
        1 aaaaaggcgg gaaagatggc atcacaatga catttatcgg cccttccccc tctgttcagg
       61 acaaatcagc cagcactgat aataccaaca tcaacggcga agacatgact gagattgagg
      121 agagcatgct tctacctgtc tccggtcagg aactgcccat tcgttggctt gctcaacacg
      181 gcagcgaaaa accagtaacg cacgtttcac gcgacgaact ccaggcatta cacattgcac
      241 gggctgaaga actaccggct gttactgccc tggctatttc gcataaaacc agtctgctcg
      301 actcgctgga gattcgcgac ctccacaaac tggttcgtga cactgacaaa gttttcccta
      361 atcctggtaa ttcagacctg ggactaataa ctgctttttt cgaagcatac ttggacgctg
      421 actacactga tcggggcctg ctgacaaaag agtggatgaa aggaaatcgt gtttcacgca
      481 tcacccgcac ggcttccggt gctaatgctg gtggtgggaa caaaaccgat cgcaatccga
      541 atttagtaca caccctcgac acactggatg tggagattgc agcagccaca cttccgatgg
      601 attttaatat ttatgaaatt ccgggcagcg tttatcgtcg cgcaaaagaa gtagtcctga
      661 aaaaagaaag tccgttcaaa gaatggtccg cagcacttcg tgcacccccg ggtattctgg
      721 actattcccg cgccgctatt tttgcactta tccgaagcgc acaccctgaa ttttatcact
      781 acccgggacg ccttcagggg tatatcaacg cctatttgac ggaaactgat cacgagaacc
      841 ccagcaagga aactctcaca gctacccggc atacgccgga aaaagatatc ctggaagaaa
      901 ttaaccgcga ggtggttact gagcgtgaaa cagaagaaga aaaaccacaa ccatctgacg
      961 caatggcagg tgaacaggca acaactgaaa caatggaacc ggatacaact gaacattgcc
     1021 agaacgcgca gtcgctggat gctcagtcgc aggtgagttc cgctaaccaa gtaaaagtca
     1081 ccgctgacga agtaaacaaa attatgcagg cagccaatat cagccagcct gacgccgata
     1141 agttacttgc tgtatcgcgt ggtgaatttg ttgaggggat tagcgaccct aatgatccga
     1201 aatgggtcaa ggggatccag actcgcgatt ctgtgaacca gaaccagcat gaatcggaac
     1261 ggaacgacca aaaagcggaa caaaacagcc caaatgcgtt acaaaacgag ccagaaacga
     1321 aacaatccga
//
LOCUS       DAJTNN010000334         1326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTNN010000334 DAJTNN010000000
VERSION     DAJTNN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.345"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..483)
                     /locus_tag="OO150_004225"
     CDS             complement(1..483)
                     /locus_tag="OO150_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5518136.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCIQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="OO150_004226"
     CDS             complement(474..776)
                     /locus_tag="OO150_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5518137.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1048..1320
                     /locus_tag="OO150_004227"
     CDS             1048..1320
                     /locus_tag="OO150_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518138.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          392 a          331 c          281 g          322 t
ORIGIN      
        1 tcagacctta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctggata caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgcctgaa ccacggcagc ctggcggctg caaatcgggg tgtggcagaa tatgagcttt
     1021 cagagtaacc atccagaatg agttcccatg tcacacaacc tggagcacca gaaagtccac
     1081 accagaatgg tgaaagaggt tctgaaagcc gtcgccagag ccaataatca cccttataag
     1141 tccgtatttg cagactttat caccggtcat ccctcctgca cggtctgttt ctgggaaacc
     1201 ttccacaaaa tgtacccgga ttcaccgtac gaatacgtca cgttctgcca tacctgccgt
     1261 cgttttgatt tatacgaaac agaagcggag atgaaggcgg atgacccgaa gtggtggtga
     1321 ttttca
//
LOCUS       DAJTNN010000335         1325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTNN010000335 DAJTNN010000000
VERSION     DAJTNN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.346"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..77)
                     /locus_tag="OO150_004228"
     CDS             complement(<1..77)
                     /locus_tag="OO150_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5518139.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPE"
     gene            complement(128..472)
                     /locus_tag="OO150_004229"
     CDS             complement(128..472)
                     /locus_tag="OO150_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5518140.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            complement(477..692)
                     /locus_tag="OO150_004230"
     CDS             complement(477..692)
                     /locus_tag="OO150_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5518141.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(769..1116)
                     /locus_tag="OO150_004231"
     CDS             complement(769..1116)
                     /locus_tag="OO150_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR5518142.1"
                     /translation="MIKGPLRKSAPVSQPEANPKNPLLLNNPFQRENPMSEITSLVTA
                     EAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDAILDELLGVAAVPEPEGIAGDGSAS
                     DGGEPTPDSDMMM"
     gene            complement(1040..1222)
                     /locus_tag="OO150_004232"
     CDS             complement(1040..1222)
                     /locus_tag="OO150_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCR5518143.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
BASE COUNT          320 a          382 c          277 g          346 t
ORIGIN      
        1 atttcaggcg caggcgcacc tgcggcaatc agcgccagaa cggcagccga caggccgtat
       61 ttgattttgg tgttcatgga tatttatcag ggtttatcga tttcaaatcc ctggatatgt
      121 taagtcttca ggccagcggt ggagtcttca gagaaccagt aattattccc ggtagttttc
      181 ctctgtaggt tatcaacaca tcctgcgcct ctaaaattac ggggcgcttt tccggcaacg
      241 gaccatcccc ttcacataac ccggcagcaa catccatgaa aaactgcttc gcctgctttt
      301 tcgcctcagc ttcgtaaaac tccagcgtgg catcttcagt acggtcaaga ctaatcgcca
      361 catctggcaa caacagtgac ggatacccac caatttccag tgccacagta acagtaatct
      421 tattcgggta attatttatc cctttaacaa ccagttcgta ttttttcttc atcgctttac
      481 tctccccgcg ccgccttacg acggtcctct ctgattttga aatacaggtt agtcagatat
      541 gtcagcagcc caaacaacag actccccagc acgcctattg ccgcccactg agacggggaa
      601 aacctgtcca gcaactgcag gaaccagtag cccgttccca ccgctgacgt ggtgtatgac
      661 acacctgttg tgattttttc catctggtac ataccccgtc tcccgcaatc cggaagctca
      721 caacaatata aagaccaccg gcacacaccg atggtccctt gcgcatgctt acatcatcat
      781 gtcgctgtca ggtgtaggtt caccgccatc tgaagcactc ccgtcacccg cgataccttc
      841 cggctcagga accgctgcta cgcccagcag ctcatccaga atggcatcca cttctgcatc
      901 aagacgcgac tcaagattct ggcgaagttt ctgtttcagt gcgctccgga cttcttcaga
      961 gcgcaggact tccttcactg cctctgcagt gaccagggat gtgatttctg acatgggatt
     1021 ttctcgctga aaggggttgt taaggagtaa cgggttcttc gggtttgctt ccggctgact
     1081 gactggcgct gattttctca gcggcccttt tatcaatctg cctgcaccag aaatcgcgca
     1141 cagccctgta cccacccgaa agaagataca gcacacagac cgccgtacag aagtacagca
     1201 tcacctgatg aataaatgtc ataatttctt accgttatgg ttgacaatga gaattgtttt
     1261 catttaaaaa accaatgtac gaaagcatct tttctttaca ttctccattg ggattacctc
     1321 cgcca
//
LOCUS       DAJTNN010000336         1321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTNN010000336 DAJTNN010000000
VERSION     DAJTNN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.347"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..695)
                     /locus_tag="OO150_004233"
     CDS             complement(<1..695)
                     /locus_tag="OO150_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5518144.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
     gene            complement(1011..>1321)
                     /locus_tag="OO150_004234"
     CDS             complement(1011..>1321)
                     /locus_tag="OO150_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCR5518145.1"
                     /translation="SISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDG
                     VVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK
                     "
BASE COUNT          385 a          227 c          282 g          427 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac gttaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagttct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatatcctg
      721 ataaaatgtg atactgggaa ataataagct aaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaagt gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa tgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc
     1021 cctgaagcag aaaacattac tacagtacct tgaaccttgg ttttaacatt ctcgaaatgc
     1081 tctgagagta tatgtgttaa gccttcttcg gaatcttttg tgtttgaaaa gatgcctttc
     1141 tgattgtaaa tgcgcatcag tttttgaccg aagctattgt gcacaactcc atcgccaaga
     1201 attgtggctc cgtatagagt tccatcatca gttaaggcct gcgccgcatt gcgtattaca
     1261 cagctttttg tagatatatt tccaggcagg cagtgaagaa ggtaaaacat ggaaatggaa
     1321 t
//
LOCUS       DAJTNN010000337         1304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTNN010000337 DAJTNN010000000
VERSION     DAJTNN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.348"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            342..>1304
                     /locus_tag="OO150_004235"
     CDS             342..>1304
                     /locus_tag="OO150_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5518146.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAALNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWSIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVA"
BASE COUNT          264 a          285 c          363 g          392 t
ORIGIN      
        1 ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc agtggtacgt
       61 ctgaaatcga tcagacgcga tgcattgctc tgaaagcata gacgggaaat atgagtttgc
      121 tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag ttcaataaaa
      181 acaatcagat gagactaatc atctcttcag cattagcgtt atgaatgagt aatataaatc
      241 gataataccg ccgctgggat atcgcgtatt ttcactcatt gacaatgttt tggcggtggc
      301 atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc
      361 ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgattgcg gcgttaaata
      421 cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta ggcgttgtca
      481 gctcatccta ttttaccggc aaccttgtcg gtacattgct gacagggtat gtcattaagc
      541 gcattggctt taaccgcagc tattatctgg cctccttcat ttttgccgct ggctgtgccg
      601 gccttggcct gatgattgga ttctggagct ggttggcttg gcgttttgtc gcgggcgtcg
      661 gctgtgccat gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg acgtcacgta
      721 accgtgggcg tttgcttgct gcgtatatga tggtttatta cgtgggaacg tttttaggcc
      781 agttactggt cagcaaagtt tcaaccgagc tgatgtccgt attgccgtgg gttacaggtt
      841 tgacgttggc agggatctta ccgctgttgt ttacgcgtgt gctgaatcag caggctgaaa
      901 accatgattc gacgtcaatt acgtcaatgc taaaactccg tcaggcgcgg cttggcgtga
      961 atggctgcat tatctcagga atcgttctgg gatctctata tggcctgatg ccgctgtacc
     1021 tcaatcacaa aggggtgagc aatgccagca ttggtttctg gatggcggta ctggtcagtg
     1081 cgggtatcct tggacaatgg tcgattggac gtctggcgga taagtttggt cgactgctgg
     1141 tgttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat cgcgatgctt agccaggcgg
     1201 cgatggcccc agcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga
     1261 tggcatgggc ttgcgagaaa gttgaacatc atcaactggt ggcg
//
LOCUS       DAJTNN010000338         1288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTNN010000338 DAJTNN010000000
VERSION     DAJTNN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.349"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            209..1156
                     /locus_tag="OO150_004236"
     CDS             209..1156
                     /locus_tag="OO150_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5518147.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
BASE COUNT          347 a          252 c          315 g          374 t
ORIGIN      
        1 cgtcatgctc gtctactgtt gttctggttg ttggtgatgc ggtggcgctt gcactctctg
       61 aattgaaaaa atttactcgt gctgatttcg gcttatatca tccaggaggt gcactcggca
      121 ttaaagcaaa ttcataattt caaagtataa atacagtgac cggtcacgcc atcttttggg
      181 gtggcgttca ttgaagtgag tgtcagctat gaaaaatgtt ctggtcaccg tccccagctt
      241 ttcagcacgc tgtgtttccg ccagcaagtt gctaagagag aataatttta atcttattat
      301 taagaataat gtggagcatc tgcttaaatc agaatccact gcattacgag aatctatttg
      361 cgcggtcatc gccggaaaag attgttatca ggcggacacg ctatcattgc tgcctggtgt
      421 gaaaataata tcgagattcg ggacggggat agataatatt gatcttcgtg cagcacaaca
      481 atccggtatt gtagtcaata atgcggttgg gattaattca aatgctgtcg cggaatttat
      541 cattggactt atcttcgcga gcatgagaaa tattcccggc agctatcatg cgatgcaaaa
      601 tggctactgg ggtgagtcgc atggctatga attacaaggc aaacggattg gcatggttgg
      661 ctacggcaat ataggtaaaa ccctggcaaa aaggttatcc ggttttgatg ttgagctttt
      721 agcttatgac aaacaacccg attatcaggt tgctgacaaa gttggagttc agtttgtatc
      781 aattgaagat atctttatgc agtcgcatgt catcattgtt cttttgcctt tttctcctga
      841 gctggagaac ttcattagtc ataaatattt gtcgatgatg cgcaatggtg ctctaatcat
      901 caatgccgca aggggaaagt tgctggatga gggcgcttta ctacaggtga ttgaagaacg
      961 aaatgtgttt gcggcgctgg atgtgtttag ctctgaaccg ttggcgcaat ttagcccact
     1021 gctacatgcc aaaaatatca ttacaacgcc gcatattgcg gccgccaccg ttgagtcata
     1081 ccagcaaacc ggtacacacg ttgcccagtc gataattgat tacttcgcag gaagggagat
     1141 aaaaaacgtg ttgtgagttc agcgaacgaa gtttttaaat acaaaaaaac cgcaatttgt
     1201 ggaacacatt gcggtttatc agcattctaa gtattggttg cgggggccgg atttgaaccg
     1261 acgaccttcg ggttatgagc ccgacgag
//
LOCUS       DAJTNN010000339         1283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTNN010000339 DAJTNN010000000
VERSION     DAJTNN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.350"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            221..>1283
                     /locus_tag="OO150_004237"
     CDS             221..>1283
                     /locus_tag="OO150_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5518148.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPGTYDRPDPRIKQLARRST
                     VTPGGAACKYNDIILADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALE
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIR
                     GIGYYGSKWRDSSATWTSQD"
BASE COUNT          310 a          312 c          381 g          280 t
ORIGIN      
        1 aaaaagcccg cgctgggaga cgcgggcggc aaggaataaa caataaaacg tgaagtaata
       61 tttcagctgg cgaataatac cccatagtag tcactctgcg caactgcgcg gtctttttcg
      121 aattgcgggc tgtcgtctct cttctgccat tgtcctgtaa cttccggact tcagcccgct
      181 cctcatttta ctcacaatat tatcccggcc gggaggattc atggctttta aacactatga
      241 tgttgtcagg gcggcgtcgc cgtcagatct tgcggaaaag ctgacacata aactgaaaga
      301 gggctggcag ccgtttggta gtccggtggc cataacccct tatactctga tgcaggcgat
      361 tgcagcagaa ggtgcagtaa tcagcgccac cagcgacccg gagtattact ttgttgtggt
      421 tctggcaggg cagtcaaacg gcatgtcgta tggtgaaggc cttccgctgc cggggacata
      481 tgaccgtccg gacccgcgta ttaagcaact ggcgcgtcgc agtacggtga caccgggcgg
      541 tgcagcatgc aaatataacg acatcattct ggcggaccat tgtctgcatg atgtgcagga
      601 catgagccgt cttaaccatc cgaaagcgga cctgtcaaag gggcagtacg gaaccgtggg
      661 gcaggggctg catatcgcca aaaaactgct gccgtttata ccggcgaatg cgggcattct
      721 gctggttccg tgctgtcgtg gtggttcagc gttcaccacc ggagctgatg gcacatacag
      781 tgacgcgagt ggtgcctcgg agaattcaac ccgctggggt gtggacaagc cgctgtataa
      841 ggaccttatc ggtcgaacaa aagcggcact ggagaagaac ccgaaaaatg tgctgtttgc
      901 cgtggtgtgg atgcaggggg aatttgattt tggcggtacg ccggcaaatc atgccgcaca
      961 gtttggtgcg ctggttgata aattccgtgc agacctggcg gatatggcag gccagtgcgt
     1021 cggtggctct gctggcggtg ttccctggat atgcggggac acgacgtatt tctggaagca
     1081 gaagaacgaa tccacgtacc agacggtgta cggcagctat aaaaataaaa cggaaaagaa
     1141 tatccatttc gtaccgttca tgaccgatga gaacggggtg aatgtgccga cgaacaaacc
     1201 ggaagaagac ccggacattc ggggtatcgg ttattacggt tcgaaatggc gtgacagctc
     1261 agccacctgg acgtcacagg aca
//
LOCUS       DAJTNN010000340         1278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTNN010000340 DAJTNN010000000
VERSION     DAJTNN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.351"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..581
                     /locus_tag="OO150_004238"
     CDS             <1..581
                     /locus_tag="OO150_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCR5518149.1"
                     /translation="EFGVSERTLQRDFQQRLIHLDLEHSSGSYRLTCRTGREHSPNTF
                     AFIRNSGISGIIPAQNRRLIRLITSNSGSSPCLIGHGGLPSPVTQTACFLELAEAIRD
                     YRAVTLLVNGKSYEAVAPRRLIFQSKNWFLVATRTRNLQVFRMEDVNAVTVLDTSFRR
                     KPELDALTASEEFISALPHFRFISDVIQTFRE"
     gene            620..>1278
                     /locus_tag="OO150_004239"
     CDS             620..>1278
                     /locus_tag="OO150_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR5518150.1"
                     /translation="MNDPVKGEGRMITDASGVTRREGGILGMWSNIFPERNGSNEQKG
                     DSKPHIPDIRDILDIFPQPLREKLLNKISEAIDYEPVIGVMGKTGAGKSSVCNALFKG
                     EVCAVSDVEACTREVQELRIRFGKHSLKIIDIPSVGENARRDKEYEYLYRNLLPSLDL
                     ILWVIKGDDRAFSADEHFYNNVLLPAGGGERVLFVLNQVDKIEPFREWDTHLHQPSPA
                     QR"
BASE COUNT          334 a          298 c          321 g          325 t
ORIGIN      
        1 atgaatttgg tgtctcagaa cgcaccctac agagggattt tcagcaacgc ctgatccatc
       61 tggatctcga gcattccagc ggaagctacc gacttacctg ccgaacgggc cgggagcact
      121 cacccaacac atttgcgttc atccgtaatt ccgggatttc agggattatt ccggctcaga
      181 atcgcaggtt aattcgactg attaccagta actccggaag ctcaccgtgc cttatagggc
      241 atggagggct gccatctcca gttacacaaa ccgcctgttt tctggaactg gcggaagcta
      301 tccgtgacta ccgcgccgtt acattattag ttaacggtaa aagctacgaa gcagtagctc
      361 cccgccgtct gatatttcag tccaaaaact ggtttcttgt tgctacccgc accagaaatt
      421 tacaggtgtt tcggatggaa gatgttaacg ccgtaaccgt tctggatacc tcttttcgca
      481 gaaagcctga acttgatgct ctgactgcca gtgaagagtt tatttcagcc ctccctcact
      541 ttcgtttcat aagtgacgta attcagacct tcagagagta acagacaacg ttcatttcaa
      601 attaaaaagg agacctcgta tgaatgaccc ggtaaagggc gaaggccgga tgattactga
      661 tgccagtggc gtcaccagaa gggaaggcgg gattttggga atgtggagca atatatttcc
      721 agaacgtaat ggtagcaacg agcaaaaagg tgatagcaag ccgcacatac ccgacatcag
      781 ggatattctt gatattttcc ctcagccact gcgggaaaag ctgttgaaca aaatcagtga
      841 agccatcgac tatgaacccg tgattggtgt catgggaaaa actggcgcgg ggaaaagcag
      901 cgtatgtaat gcgctgttta aaggggaggt ttgtgcagtt agcgacgttg aggcatgtac
      961 tcgcgaagtt caggaattgc gtatacgctt cggcaaacat tcgctaaaga tcattgatat
     1021 acctagtgtc ggtgagaatg cccggcggga caaggagtat gaatatttat accgtaattt
     1081 gcttccctcc ctggatctta tcctgtgggt aataaaagga gacgatcggg ccttttctgc
     1141 ggatgagcat ttctacaata atgttctgct ccctgctggt gggggtgagc gcgttttatt
     1201 cgtcctcaac caggttgata aaattgagcc attccgcgag tgggatactc atctgcatca
     1261 gccatctcct gcacagcg
//
LOCUS       DAJTNN010000341         1265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTNN010000341 DAJTNN010000000
VERSION     DAJTNN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.352"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            161..751
                     /locus_tag="OO150_004240"
     CDS             161..751
                     /locus_tag="OO150_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR5518151.1"
                     /translation="MDSLQDDYTKLLYGLMQPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPVDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            738..>1265
                     /locus_tag="OO150_004241"
     CDS             738..>1265
                     /locus_tag="OO150_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5518152.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRFATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTIN"
BASE COUNT          316 a          277 c          367 g          305 t
ORIGIN      
        1 gtatgtatct gtccagaatc agtgaggcca tcagtttgtc tgctggtgaa gtggcccacc
       61 ggttaatcgt cccttcatcg gatattgacc tgggggagac cgagcttccg gtgctcggcg
      121 agatcacctg gcaggcttat gacccggcaa ggagtaaata atggattcgt tacaggatga
      181 ttatacaaag ctgctgtacg gactgatgca gccggggcct gcatggtcag ataccgacgg
      241 tgtacttgac ggtctggcac catcgcttgt gcgtgttcac cagcgggctg atgaactggt
      301 gattgaaatc gatcccggtc agtccacaga gctgattgaa cgttatgaag aattgtatgg
      361 tttacctgat tcctgttccc ctgtcggaac ccagacatta cgccagcgtc agcaacgtct
      421 tgaagcaaaa gccaatgtgg ctggtggcat aaatgagcag ttcttcctgg atcagcttga
      481 ggcgctggga tataccggcg taacgatcga acagttccag cacctggatg caagccccga
      541 tccggaatgg ggagatcgct ggcgttattt ctggcgtgtg acgttgccgg tggatgccgg
      601 tgctcagtgg cagacatgca cggacgcctg caacacaccg atccggacgt ggggggatac
      661 ggttgcggag tgcgtgatta ataaattatg tccgtcacac accgtcgttt tattttccta
      721 tccagatgag gatgaagatg cacaggattg atacgctgac tgcggtaaaa gataagtttg
      781 gtccgggtaa gaacggattt actgacggaa atcttcgcac aggacgtttt gctacctggc
      841 tgaacagtgc catgtggaat gccattcagg aggaaatctg tggtgtcatt gagaaggccg
      901 ggatagaact gaataaagaa gaacacgacc agctatataa ggctatatta ttgctggtgg
      961 gcggtgcaat taacgaagag gcattgctga taaaaaataa cctttcggat gtggaagaca
     1021 gggatgaggc tgttgaaaac ctcggattaa aacccacggt ggataaggca aaaaatgccg
     1081 ttcagcgtga tggcgacacc atgaccggag agctgaaaat ccgtggtgtt aatgcgctga
     1141 ggattttcaa cgaagctttt ggcctgattt ttcgtcgttc ggaagagtgc ctgcacctta
     1201 ttcccaccag tgaaggtcag ggggaaaatg gcgatattgg tcccctgcgc ccgttcacca
     1261 ttaat
//
LOCUS       DAJTNN010000342         1260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTNN010000342 DAJTNN010000000
VERSION     DAJTNN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.353"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..562)
                     /locus_tag="OO150_004242"
     CDS             complement(<1..562)
                     /locus_tag="OO150_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR5518153.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWK"
     gene            932..>1260
                     /locus_tag="OO150_004243"
     CDS             932..>1260
                     /locus_tag="OO150_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518154.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
BASE COUNT          328 a          324 c          309 g          299 t
ORIGIN      
        1 ctttccagcc gatatcttcc agttccgcca gcgtggagag cgcctcatcg tactcatcgc
       61 cctgatgccc ttccataatc acccggctca actgctccag gtcgctatag atattttcaa
      121 tttcatctgc caactgttcg attttggttt cgaacagatc cagcagcagc tcgtaggcgt
      181 taccgtctac catcgactgg ctacgggcac gcatacgata cagacgaaaa gcgggcagtt
      241 cacgctcacg cagagtaaac agacgaccat cacggatggt aaatgccaca gtggagttac
      301 cggcgtgatc ttccgcatct tcaaagaaga agaaggagtg aatatgcagg ccgtcgtcgt
      361 cttcaaagaa acgtgccgat gcttcgatgt cttccagttc cgggcgggtt gccaggctct
      421 ggccaagttc agattgtaca cgcagtcgct cgtcgtcgtc cggttcgaca agatcaatcc
      481 atactgcatt tacaaggggt tgtgactctt cgacttccag ccgggtcagt cggttatttt
      541 ccagttgaaa tgcgctcagc atgaccggga ctcccaatgc ttaaaatatc ggacagttcg
      601 gtgggcacac agaaacaaat tgggtttcag accattaaac agcctgactc agcgcgacgg
      661 gaaaaattga ggtcgctgac aaccgctaag gctatcagca aaaagggata gccttaggag
      721 ttgatcctgg atgacaggat agtgagccag tatctactgg gtgtgtccaa ggcgaatgtc
      781 ctcttagaat gatcgtgcgc gcatgttacg ccagcaaaat tttgccgtca acacgcaaca
      841 gaacaccaca gaacaatagt tgaacttcac gctttttggt actcctcaag gtcgctaaca
      901 ggataaaaaa atcccacatc caggacctat aatggcgcat cggctactca ttggtaaagg
      961 aatgatcacc ttgaatctca aacggatttt tctcaccctc accttacttc ccttgttttc
     1021 tgttgccgct gatgattgcg cactctcaga tccgacgctg accgtacagg cgtataccgt
     1081 taatcctcaa acagagcggg tgaaaatgta ctggcaaaaa gccaatggcg aagcgtgggg
     1141 aacgttacat gctctgctgg cggatattaa tagtcagggt caggtgcaga tggcgatgaa
     1201 cggcggcatc tatgatgaaa gctatgcgcc gctcggtttg tacatcgaaa acggtcagca
//
LOCUS       DAJTNN010000343         1213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTNN010000343 DAJTNN010000000
VERSION     DAJTNN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.354"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..876
                     /gene="lpxM"
                     /locus_tag="OO150_004244"
                     /gene_synonym="msbB"
     CDS             <1..876
                     /gene="lpxM"
                     /locus_tag="OO150_004244"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5518155.1"
                     /translation="TWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKARRRATIN
                     LSLCFPEKSDTEREIIVDKMFATALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIR
                     YNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFHQQRNPVIDYVWNSVRRKFGGRLH
                     SREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAM
                     IIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMIARQMNKTVEILVGSHPEQYIWVL
                     KLLKTRKSNEADPYP"
     gene            complement(1030..>1213)
                     /locus_tag="OO150_004245"
     CDS             complement(1030..>1213)
                     /locus_tag="OO150_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5518156.1"
                     /translation="IYQPVYSPWVNHAERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
BASE COUNT          337 a          248 c          291 g          337 t
ORIGIN      
        1 acatggttcc ttttgggaat gattgcaggt atttcaatgt ttcccccttc attcagagat
       61 cctgtcttgg caaaaatagg gcgttgggcg ggcagattga gcaaaaaagc tcgtcgcagg
      121 gcgacgatta atttatcgct ttgtttccca gaaaagagtg atacagaacg ggaaataatt
      181 gtcgacaaaa tgtttgccac agcattgcaa tccatagtga tgatggcaga actggcaatt
      241 cgtggtccgg aaaagttcca gaaacgagtg ttctggaaag gcctcgaaat tcttgaggaa
      301 attcgataca ataacagaaa tgtgattttt ctggttcccc atggctggag cgtggatatt
      361 cctgcaatgt tgctggcagc tcagggggaa aaaatggccg ccatgtttca tcagcaacga
      421 aatccagtga ttgattatgt ctggaattct gtacggcgta aattcggggg gcgcttacat
      481 tcccgggagg atgggataaa accatttatt cagtcagtac gccagggata ctgggggtat
      541 taccttccag atcaggatca tggtcctgaa tacagtgaat ttgctgattt ttttgcgacc
      601 tataaagcga cattaccaat tattggacgt ctgatgaaca tcagtcaggc tatgattata
      661 ccgcttttcc cggtttatga tgaaaaaaaa catttcctga ctattgaagt tcggccacca
      721 atggatgcat gcattgccag cgcggacaat aaaatgattg cccgacaaat gaacaaaaca
      781 gtggaaattt tggtggggtc acatccggaa cagtatatct gggttttaaa attgttaaaa
      841 acgcgcaaat caaacgaagc ggacccgtac ccttgaaatt ataaagtaca ggtatttcca
      901 ctagttgttt cttacaggtt accaatcgaa acacatcccc cttccgtgtg ttgcccgttt
      961 ggcacggagg actgtgtaga tgcagttcgt taaatttaat ttatgcctaa atagctgcgc
     1021 ttaataccgc tacacttttg ccagtccatg tttgcctccg gggaatgggc tgacggtttc
     1081 cataaaatgg cgaacttttt tcaacagttg ccacattgag cggcactgat gattacgcgt
     1141 tattgtgtcg tgaagtgcct gccatagccg ttcagcatga ttcacccatg gcgagtaaac
     1201 cggctgataa atg
//
LOCUS       DAJTNN010000344         1180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTNN010000344 DAJTNN010000000
VERSION     DAJTNN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.355"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..717
                     /gene="rlhA"
                     /locus_tag="OO150_004246"
     CDS             <1..717
                     /gene="rlhA"
                     /locus_tag="OO150_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR5518157.1"
                     /translation="ELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNAEKAMNNLKD
                     GLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASYQRGSRKPVA
                     EPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPVMITKHCLRF
                     AFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKNHILKMPLPG
                     SVVASVSPDELLKTLPKRKG"
     gene            complement(809..1039)
                     /locus_tag="OO150_004247"
     CDS             complement(809..1039)
                     /locus_tag="OO150_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCR5518158.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT          301 a          305 c          285 g          289 t
ORIGIN      
        1 gaactgggcg gctggcagga acaactgatt ctgaccctca ccagtgaaga gggtgtcagc
       61 atcacgcata cgctggacgg gcagttcgac gaagccaata acgccgaaaa agcaatgaac
      121 aatctgaagg atggtctggc aaaactgggg caaaccctct attacgcccg cgatgtgcaa
      181 attaatttgc cgggggcgct gtttgtacca aacagtctgt taaaccagtt ccgccgtgaa
      241 gctgctgaca tgctggatgc tgcgcgtctt gccagttacc agcgcggcag ccgtaaaccg
      301 gttgctgagc ctgcgccggt ttatccgcaa acgcatctga gtttcctcgc gaacgtatac
      361 aaccagaaag cgcgtgaatt ttatcatcgc tatggtgtgc agctgattga cgcggcgtat
      421 gaggcacatg aagagaaggg cgaagtcccg gtgatgatca ccaagcattg tctgcgcttt
      481 gcctttaatc tgtgcccgaa acaggcgaaa ggcaatatca aaagctggaa ggcgacgcca
      541 atgcaactgg ttaacggcga tgaagtatta acgctaaagt ttgattgccg cccatgcgag
      601 atgcacgtca ttggcaaaat caaaaatcac atactgaaaa tgccgttacc gggaagcgta
      661 gtggcatccg taagtccgga tgagctgctg aaaacattgc ctaagcgaaa agggtaaaac
      721 gccagttttc tggttactca caactcattg aatctgcatg atattgcctg ccgggtaagg
      781 cgtcacgccg catccggcat caaacgactc aacgttgtcc cgcttctggt ttgcgcgatt
      841 tttgccagta aaaatgctcg cgcaaacctt ccgccgactc ttccgccaca gcacgcaatt
      901 catcgctgtc cgcttcatga cgcagctgat gatccacatt ctttacccac acaaattcat
      961 gtcctttgtg ccctgccatg agttgtcctg agaacagcgc acacgttaat aagacaaccg
     1021 ataacgcctt cgtaaacatc ctgccacctt tttcttacct tttgccgcta tgatgccgat
     1081 cgtttcttga ggttattatt cagttttgca aattagcgca aagaaattct ggaatcttcc
     1141 ttcctgattt tgcattgcat tctgtcgttg cggcgattta
//
LOCUS       DAJTNN010000345         1154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTNN010000345 DAJTNN010000000
VERSION     DAJTNN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.356"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..265)
                     /locus_tag="OO150_004248"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="OO150_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO150_004249"
     CDS             complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO150_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5518159.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          337 a          284 c          176 g          357 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactctt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tgttttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gagtctaaaa
     1141 agtgatccac ttac
//
LOCUS       DAJTNN010000346         1136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTNN010000346 DAJTNN010000000
VERSION     DAJTNN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.357"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..112)
                     /gene="ariR"
                     /locus_tag="OO150_004250"
     CDS             complement(<1..112)
                     /gene="ariR"
                     /locus_tag="OO150_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCR5518160.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQA"
     gene            complement(141..413)
                     /locus_tag="OO150_004251"
     CDS             complement(141..413)
                     /locus_tag="OO150_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCR5518161.1"
                     /translation="MKTSDNERIKYEITGQVVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(456..692)
                     /locus_tag="OO150_004252"
     CDS             complement(456..692)
                     /locus_tag="OO150_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCR5518162.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANWPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            1006..>1136
                     /locus_tag="OO150_004253"
     CDS             1006..>1136
                     /locus_tag="OO150_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HCR5518163.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     "
BASE COUNT          340 a          235 c          187 g          374 t
ORIGIN      
        1 cagcctgccc taacactgct gactcttctt ctaacaaatt acccgaactg cgaaagtaac
       61 ttgctaacgc tttatcagtt attgcattat gaattgtagt atcttcaagc atggtaacct
      121 ctcatcttac ttatgaaatt ttaatgtatt ctgtttattt tcttaccatt gaacatactt
      181 ttattgtcag ctgtcgctgt tcttcggttt ggccctcctg aatcactatt gctaaaatca
      241 cgaattatgc tgtcaattaa atcgcgtcgg aaagcatctt cctcagccga tttcattacg
      301 agtaattgct taataagagt ctgcaatgaa aaatttatct tcatgcgcag tatctggagc
      361 accacctggc cagtaatttc atattttata cgttcattat cagatgtctt cataacgact
      421 tcatagctaa cctgttaaat aatcaggctg ttgcattatt caaaaagcaa cccaattagt
      481 gcattataat gtttctgttc ctcttcccca gtcgcgtgtt ccagtcggca aagaagtttg
      541 gcacaaagcg actttcgact cagattacgt ccatctttca aaatctcagt cacaatttcc
      601 ccgagagttt cctgctgagt aggccagtta gcttttgcaa agtaacgagt gattgctccc
      661 gcagaatcta aagtcactga attttgatgc atgctacgcc tctgttaaaa atgttaatca
      721 atgttaagtg aggtaacaat agcaaacttg tacgaaatat gtgtacagaa tgataaatta
      781 attttttgca attagtgcca atacatataa aacaatgtgt tatgataaaa catattgcat
      841 catttaactg acaatctata cgagattttt cgccaactag tgttcaactt tttccatttt
      901 ttatcaatgt aaagaaacgg ttatgttttg gtattaaaag aaagacacaa gaagatatgt
      961 ctgttaccgt cttactctcg cctcacccat taccctggat tgttaatgct taccaccctt
     1021 atttatcgta gccatatacg tgacgacgaa cctgtcaaaa aaatcgaaga aatggtttcg
     1081 atagcaaatc gcaggaacat gcagtctgac gtaacaggga tcttactgtt taatgg
//
LOCUS       DAJTNN010000347         1124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTNN010000347 DAJTNN010000000
VERSION     DAJTNN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.358"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..170
                     /locus_tag="OO150_004254"
                     /pseudo
     CDS             1..170
                     /locus_tag="OO150_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            187..705
                     /locus_tag="OO150_004255"
                     /pseudo
     CDS             187..705
                     /locus_tag="OO150_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          325 a          263 c          247 g          289 t
ORIGIN      
        1 tcgtcggtcg tgacagactg gcacgtcttc gtaaggagct gaggctgcac tgtaagcaga
       61 aacgcaagtt cagggcgacc acaaactctg accacaatct gccggttacg ccgaatctgc
      121 tgaatcagaa ctttacgccc acagcaccta atcaggtctg ggtcgcagat tcagttgtcc
      181 aggccttccg aaatcaaccg actgaaggcg ctggtcgcga aactgcagcg tatgcagttc
      241 ggtaaaagct cagaaaaact tcgcgcaaaa accgaacggc ggatacagga agcacaggag
      301 cgaatcagcg cacttcagga ggaaatggcg gaaacactgg gtgagcaata tgacccggta
      361 ctgccatctt ccctgcgcca gtcttcagcc cgtaaaccgt taccagcctc acttccccgt
      421 gccccccggg tcatccggcc ggaagaggaa tgctgtccgg cctgtggtgg tgaactcagt
      481 ccactgggat gtgatgtgtc agagcagctg gagcttatca gcagcgcctt taaggttatc
      541 gaaaaacaac gccctaaact ggcctgtcgc cggtgcgacc atatcgtgca ggcaccggtg
      601 ccttcaaaac ccattgcacg cagttatgcc ggagcggggc ttctggccca tgttgttacc
      661 ggaaagtatg cagaccatct gccattatac cgccaaagtg atctactatt ccacacagct
      721 atttagctct ctttttgttg agctcccatg attctttcat ttattgttac taattatttt
      781 atgttatcat gatgttagaa cacgctctgc tctgtgtctc tattttatct atcatattct
      841 gaggattaac tacataacaa ggcgcaagat atatatccaa aaacaaataa taccgttgca
      901 ataattcctt tcagtaaagg aggtgtgatt aatttcaggg gatttcagag tgaaaattgt
      961 acaacatatt gaaatatgtt catttcgtta taatacacac aagaaaatat taagttagat
     1021 aatatattct aaataaccat agataaaaat gcacataatg gggaatattt tatgaaaata
     1081 cctgaagcag taaatcatat taatgttcag aataatattg atct
//
LOCUS       DAJTNN010000348         1105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTNN010000348 DAJTNN010000000
VERSION     DAJTNN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.359"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            154..514
                     /gene="yeaR"
                     /locus_tag="OO150_004256"
                     /pseudo
     CDS             154..514
                     /gene="yeaR"
                     /locus_tag="OO150_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
     gene            518..700
                     /locus_tag="OO150_004257"
     CDS             518..700
                     /locus_tag="OO150_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR5518164.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            848..1096
                     /locus_tag="OO150_004258"
     CDS             848..1096
                     /locus_tag="OO150_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5518165.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
BASE COUNT          295 a          234 c          278 g          298 t
ORIGIN      
        1 cgcgggcttc ccacttgtaa tcctccctga tttttctctg atatggtgct aaaaagtaac
       61 caataaatgg tatttaaaat gcaaattatc aggcgtaccc tgaaacggct ggaataaacc
      121 gttttcagcg cattcaccga aggagggaaa aggatgctgc aaatcccaca gaattatatt
      181 catacgcgct caacgccttt ctggaataaa caaactgcgc ctgccggaat aattcgaacg
      241 tcatcttgat aaaggaacgc gccagggggg ttacccacgc ctttccgtta tgcatggggc
      301 ggtcaaatat ctcggctacg ctgatgaaca cagtgcagag cctgatcagg tgatccttat
      361 cgaagcgggg cagtttgcgg tgttccctcc agaaaagtgg cacaacattg aagccatgac
      421 tgacgatact tatgtcaaca ttgacttctt cgtggctcct gaagtcctga tggaaggtgc
      481 gcaacaacgg aaagtcattc ataacgggaa atgaatcatg ggcaaagcaa cgtataccgt
      541 gaccgtcacc aataacagta atggcgtttc tgtcgattat gaaacagaga cgccgatgac
      601 tttgctggtg ccagaagtgg cggctgaagt gataaaagat ctggtgaata ccgtgcgttc
      661 ttatgacacg gaaaacgaac atgatgtttg tggttggtaa ttatcactga tcgttgatgg
      721 cgcaatttct gcgccatcgc aaaaaaatcc tccgtgatgc tcgtcacaaa tcttgccctc
      781 ccgatgttgt gctatgcttt ttatcagcga ctaacccgct aatgcaaaac agataagtga
      841 ggaaacaatg ggcattctgt catggattat ttttgggctt attgccggta ttctggcgaa
      901 gtggatcatg ccaggtaaag atggtggtgg attctttatg actatcctgc tggggatagt
      961 cggtgccgta gtcggcggat ggatcagcac gctgtttggc tttggtaaag tcgatggctt
     1021 caattttggc agcttcgtgg ttgccgttat tggtgcgatt gtcgtgctat ttatctacag
     1081 gaagattaaa agttaacgct taaat
//
LOCUS       DAJTNN010000349         1068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTNN010000349 DAJTNN010000000
VERSION     DAJTNN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.360"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..320)
                     /locus_tag="OO150_004259"
     CDS             complement(<1..320)
                     /locus_tag="OO150_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5518166.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNG"
     gene            complement(492..>1068)
                     /locus_tag="OO150_004260"
     CDS             complement(492..>1068)
                     /locus_tag="OO150_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518167.1"
                     /translation="WPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQK
                     LLTDAVKMRGLIAPASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLL
                     NAQKLRDTQHMMQQILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVV
                     ETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          243 a          256 c          252 g          317 t
ORIGIN      
        1 tcgccgtttt tcatttcgcc catagctttc agctttttgg agatatcgcg gcgttctttg
       61 gagagctcgg catttttgat gatgtaatcg tcaacgcgat cttcatagtc acctttcatg
      121 ctggcgatga tgccctggat tgcttcaacg ctcatccccg gcttgatgta atcgctcagg
      181 ttgtccagca gcaggacgcg cttctggttg tcacggatct ttttctcaac gtcctgaatt
      241 tcacgttgca gtttgttctt acgacggaac agacgaacaa attccagtac gtcctggaat
      301 gaaggcttgg tagtttccat ttttataccc ctgataatgt gagagtcgga ttcgtttaag
      361 caaccgctat ccgttagagc caacattact catagctgcc gcagatgaca atgcttttat
      421 cccttttcac tatcataccc tttatccttg ctgaatcgaa gcagcagcaa gatgattctg
      481 tagttcagga attatttgcg caaggcccgg caaatcagat tcgatagcaa ctcaagctga
      541 tgtgccgttt ccatgttcag ccacacataa ccatagattg gtgtttccac aaccttcagg
      601 tcatggtggt tattgagcaa ctgacgcagc tcttccactg catcgttcaa tttttccgta
      661 ttggcaaaaa ccggctgcgg attaccttcg tacaacgcat gaacaaggct cagcagtatt
      721 tgctgcatca tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac gaaatggctg
      781 gggcgcgtgg cccagtacgc attgatttgc aactccagca tacaaaccag attgcggtta
      841 atggtctgga taccttcata tatcgatttt ggaatacggg tttctttgct ggcgggcgca
      901 atcagcccac gcattttcac ggcatcggtc aggagttttt gtagatggct ttccagacgt
      961 gggcgttcga gtaagttcgg tgagaatgca gattgataga cccgattata ctcggtcaga
     1021 cttttcgcca gttgaatgcg ccagtggatg aacgcccgtt gtggccag
//
LOCUS       DAJTNN010000350         1065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTNN010000350 DAJTNN010000000
VERSION     DAJTNN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.361"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..504
                     /locus_tag="OO150_004261"
     CDS             <1..504
                     /locus_tag="OO150_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR5518168.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPISEIDPQGLNPLILGVVALMSC
                     GLFSSGGVIQSGQQRREQYGRKVNPEADKLTDEHKSGVNPGGNCSPQNLSDLQNEKNR
                     LCNQSRACAPQMLRKEILRRYEINLACASVRKQINNQCFGGGDKAHMEEENKAYKTAA
                     DCSGLIK"
     gene            501..1004
                     /locus_tag="OO150_004262"
     CDS             501..1004
                     /locus_tag="OO150_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518169.1"
                     /translation="MKKIIFSNESAVYDELMIHFPCQPLPHISNDIIGLNELDIVYNF
                     FQKKQWNEIANNLKIKDDSYALELGITFLPEKVFCYYIPLYIYVSLFNKNDFWVFESD
                     FIQQYLCPEYRDYDDFLNFVFNFSDIQLSIIAQFMSYESDAGFFYASKACMDFWEDYS
                     PLLHKKI"
BASE COUNT          354 a          157 c          216 g          338 t
ORIGIN      
        1 tatatcactc aggacccaat tgggctgaag gggggatgga atttttatca gtatccgttg
       61 aatccgatct cagagataga cccacaaggg ttaaatccgt taattcttgg tgttgtagca
      121 ctgatgagtt gcggtttgtt ttcatcaggt ggtgttatcc aaagtgggca acaacgcaga
      181 gagcaatatg gacgtaaagt taatccagaa gccgacaaat taacagatga acataagtca
      241 ggtgtaaatc ctggaggtaa ctgtagtccc cagaacctga gtgacttaca aaatgaaaaa
      301 aacagattat gtaaccagtc tagagcttgc gctcctcaga tgctgagaaa agaaatattg
      361 agacggtacg agataaattt ggcgtgcgct tcggtcagaa agcagattaa taatcagtgc
      421 tttggtggtg gagataaggc ccatatggaa gaagagaata aagcatataa aacagcagct
      481 gattgcagtg gcttaataaa atgaaaaaaa taattttctc taacgagagt gcagtctatg
      541 atgaacttat gattcatttc ccatgccagc cattacctca tatttcaaat gatattatag
      601 ggctgaatga actggatatt gtatataatt ttttccaaaa gaaacaatgg aatgaaatag
      661 ccaataattt aaaaataaag gatgactctt atgctttaga gttaggaatt acattcctcc
      721 ctgagaaagt attctgctat tacatccctc tgtatattta tgtctcgttg tttaataaga
      781 atgatttttg ggtttttgaa tccgatttta ttcaacaata tttatgtcct gagtatcggg
      841 attacgatga ttttttgaat tttgttttta atttttcaga tatacaacta tctattattg
      901 cacagtttat gtcttatgaa tcagacgctg gattctttta tgcaagtaaa gcttgtatgg
      961 atttttggga agattattca cctctattac ataagaaaat ttaatcgtaa aaggtataaa
     1021 tggtttaata aaaaacttgt ctactcgttg gagtagatga aaaat
//
LOCUS       DAJTNN010000351         1044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTNN010000351 DAJTNN010000000
VERSION     DAJTNN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.362"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>1044
                     /locus_tag="OO150_004263"
     CDS             <1..>1044
                     /locus_tag="OO150_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5518170.1"
                     /translation="EHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRIT
                     TPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIIRYRYDNPHS
                     DLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYR
                     AYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQGEL
                     TRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSE
                     DEGLVTHWHYDEADRLTHRTVNGETAEQWQYDKRGWLTDISHLSEGHRVTVHYRYDEK
                     GRLTGERQTVHHPQ"
BASE COUNT          261 a          306 c          317 g          160 t
ORIGIN      
        1 gaacacgcgg acggcagcgt cacgcagagt cagtttgacg ccgtgggcag gctcaggaca
       61 cagacggatg ccgcaggccg gacaacagaa tacagcccgg atgtggtgac gggcctcatc
      121 acgcgcatca ccacgccgga tggcagggca tcggcgtttt actataacca ccacagccag
      181 ttaacgtcag ccaccgggcc tgacgggctg gaaatacgcc gggaatatga tgaatggggc
      241 cgtctgattc aggaaactgc ccctgacggc gatatcatcc gctaccgtta tgataatcca
      301 cacagtgact taccctgcgc aacggaagat gccaccggta gccggaaaac catgacgtgg
      361 agccgttacg gtcagttgct gagcttcacc gactgttccg gttatgtaac ccgttatgac
      421 catgaccgct tcgggcagat gacggcggtg caccgcgagg aagggctgag tcagtaccgc
      481 gcatacgaca gccgtggaca gttaattgcc gtgaaagaca cgcagggcca tgaaacgcgg
      541 tatgaataca acgccgccgg tgacctgacc gccgtcattg ccccggacgg cagcagaaac
      601 gggacacagt acgatgcgtg gggaaaggcc atccgtacca cgcagggcga gctgacgcgc
      661 agtatggaat acgatgctgc aggacgggtc atccgcctga ccagtgaaaa cggcagccac
      721 accaccttcc gttacgatgt actcgaccgg ctgatacagg aaaccggctt tgacggccgc
      781 acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga tgaggggctg
      841 gtcacccact ggcactatga cgaagcagac cgcctcacgc accgcaccgt gaatggcgaa
      901 accgcagagc agtggcagta tgacaaacga ggctggctga cagacatcag ccatctcagc
      961 gaagggcacc gggtgacggt gcattacagg tatgatgaga aaggccggct gaccggtgag
     1021 cgccagacgg tgcatcaccc gcag
//
LOCUS       DAJTNN010000352         1010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTNN010000352 DAJTNN010000000
VERSION     DAJTNN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.363"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..481
                     /locus_tag="OO150_004264"
     CDS             <1..481
                     /locus_tag="OO150_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HCR5518171.1"
                     /translation="PYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGST
                     LGHYGVGYGPYVQLPFYGSFTLRDDGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRA
                     QLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(663..998)
                     /locus_tag="OO150_004265"
     CDS             complement(663..998)
                     /locus_tag="OO150_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518172.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTEYQNEINQLS
                     APGVNFDDI"
BASE COUNT          268 a          213 c          216 g          313 t
ORIGIN      
        1 cccttatcag gggatggtcc actttacccg ctttttcctg aacaccattt tggggatggg
       61 cggctttatt gatgttgcag ggatggcgaa cccgaaactg caacggactg aacctcaccg
      121 cttcggtagt acgcttggtc attatggcgt gggttatggg ccttacgttc agttaccgtt
      181 ctacggtagc ttcacgctgc gtgatgacgg tggtgatatg gcagatggtc tttacccggt
      241 tctttcctgg ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag ggatcgaaac
      301 ccgcgctcag ctgctggatt ccgatggtct gctgcgtcag tcgtccgatc cttatattat
      361 ggtgcgcgaa gcgtacttcc agcgtcatga tttcatcgct aatggcggcg aactcaaacc
      421 gcaggaaaat ccgaacgcac aagcgattca ggatgattta aaagatattg attctgaata
      481 agaaacaaat aaaaaaggtg agtcgcaaaa ctcacctttt ttgttgttat ccctcaatta
      541 ccacaagtaa aaggagataa accgacaatg acccggacgt aatgatgtaa aaatactgat
      601 gttcgtcact gactatttcc gatattcagg cgttgaaaaa tcaaaaaact ggaaaaataa
      661 ttttaaatat catcaaaatt aacacctggc gcagatagtt gattaatttc attttgatat
      721 tccgtctggc gattttgaag ccttttttca actttatctt cgagatcctt aaagtctttg
      781 tatttatttt caggatggtc aacgtcaact ttgttagcga ataaagtaga aattatttct
      841 tttacattat cgcgtctaaa ttttgcatcg tccacttgaa aattatatct tattgctcga
      901 cctaaacgtt taaaaacttc gttaggttct ctcagtcttt ccccgttgag ctcgtttaca
      961 acctgatgat cggaacctgt tgcaaatgat tctgccatga tagctttact
//
LOCUS       DAJTNN010000353         1009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTNN010000353 DAJTNN010000000
VERSION     DAJTNN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.364"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO150_004266"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO150_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5518173.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO150_004267"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO150_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5518174.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGTPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..>1009)
                     /locus_tag="OO150_004268"
     CDS             complement(941..>1009)
                     /locus_tag="OO150_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5518175.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          301 a          205 c          231 g          272 t
ORIGIN      
        1 gctaaagttc tgggttaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataatcat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaac ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaattg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaagg
//
LOCUS       DAJTNN010000354          994 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTNN010000354 DAJTNN010000000
VERSION     DAJTNN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.365"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..217)
                     /locus_tag="OO150_004269"
     CDS             complement(<1..217)
                     /locus_tag="OO150_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5518176.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNL"
     gene            343..912
                     /locus_tag="OO150_004270"
     CDS             343..912
                     /locus_tag="OO150_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HCR5518177.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEVTIRDITRLPGGRIFV
                     EGGNRAFECKIEDIEIIGKIISLTVKYVR"
BASE COUNT          296 a          175 c          225 g          298 t
ORIGIN      
        1 taaggtttag attgcattct gaaagcgcat caacggtcat tgctgctatg ttgatcatca
       61 ctttttcgcg ttttttatct ttgcgcagac ggtgacggat aaggcgtcca tcagccaaca
      121 tgtcattgat ggtatcgatg gatagccctg tcagctcgct atagcgttca atagtcacat
      181 gaggcgtggt aagagtgatt gaaatgttag gtctcatgat gcaacattcc tcgtttaatg
      241 atgattaatc aggacgaata cggatcgttt gtattttgtg aacaccataa acatacgatt
      301 gcacagtgaa atcgtcaaga taaaagttca cttggagtga ccatgaattt ggagaaaggc
      361 ggacgaggcg ccatagagcg catggtagaa gcttatggat tcaagactcg acaggcgttg
      421 tgcgatcatt taggaatctc taaaagtaca ctcgccacac gctacatgcg tgactcattc
      481 ccagcagaat gggtaatcca gtgcgccctt gaaacgggca cctcgcttaa ttggctcaca
      541 accggacatg gttcaaagca aacttcaggt aatacaaata ctatggaagt tgctaaatat
      601 gtattatctg atggtgcctt gcgtgaagac ggtttttata tttttgataa gggatttcta
      661 ccctctacgt ttaaaaaacc ttttgtcatc acagataaca attctgaatt tatttgtgat
      721 aaagaatttg atgacatacg tgatggtaaa tgggtaataa gtattgatgg cgaagtaacg
      781 atccgtgaca ttactcgttt acccggtgga agaatcttcg tcgagggtgg aaacagagcc
      841 ttcgaatgta agatagaaga cattgaaata attggtaaaa ttataagttt aacagttaaa
      901 tatgttagat aataccggga ggaaactatg cttggtaaag tattttttgt ggttttatca
      961 tgctctttgt tattaagccc actaactacc tatg
//
LOCUS       DAJTNN010000355          992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTNN010000355 DAJTNN010000000
VERSION     DAJTNN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.366"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..139)
                     /locus_tag="OO150_004271"
     CDS             complement(<1..139)
                     /locus_tag="OO150_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518178.1"
                     /translation="MADKAILWALISASTKEERKACSLSYFACKAAEAELGLAYMAAN
                     DN"
     gene            complement(847..>992)
                     /locus_tag="OO150_004272"
     CDS             complement(847..>992)
                     /locus_tag="OO150_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5518179.1"
                     /translation="LSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNK
                     ENP"
BASE COUNT          289 a          215 c          166 g          322 t
ORIGIN      
        1 tgttatcatt cgcagccata tatgccagtc cgagttcagc ctctgcggcc ttacaggcaa
       61 aatagcttag tgaacacgct tttcgttcct ctttagtact ggctgatatt agtgcccata
      121 atattgcttt gtctgccaaa tctaactctc tctgaaattg tcttgcagta actgcactcg
      181 cagaaataga gaaaaaaata caattgttaa taaccttgcc atattttttt agtccgtaac
      241 tgttaatgtg ctatctggct ctgaaaatat taactcattt aatttttgca tgtcattaga
      301 taaaccagtt atacaaccct cggaacagaa tcgaggtcct aaagacggac caaaagcgtt
      361 taaacaacta tgtattgaaa atccattgcg attataagtt cctgtattaa ctgacggtac
      421 taatcgtatg ccgtcaggtt ttctattgct tgctcccggg tcattaacat tccaactcca
      481 taggccttga ggtattggcc atctatttat aaatgtgtac aacggggatt atttttacat
      541 tgcatattat taccagaagc gactggaata ctaagcacat cataaaaatt atcatcacat
      601 ggtatatctg aaacacacga aagcatacct aaagttatgc tgtataaaca ggtggctaat
      661 cccaagggga tcgattccag aaatcgggtt caacggatac tgataaaaat tccacctacc
      721 ttccagccca atcggagcct gagtgttata ccgcccctgc tgcggttaat gttaccgata
      781 atagttatag tacagccctg attcatcatc atactactgc tgcggcagtc tgatgaacta
      841 ttgcttttac ggattctcct tattcttcct tctatccatt cgctttagct ttttgagctc
      901 ttgaagtttg ctctttgcat caatattacc gttatttaca gcgaattcca tccaataaat
      961 agctctattc aaattataat attctgataa tg
//
LOCUS       DAJTNN010000356          989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTNN010000356 DAJTNN010000000
VERSION     DAJTNN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.367"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..680)
                     /locus_tag="OO150_004273"
     CDS             complement(<1..680)
                     /locus_tag="OO150_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5518180.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYF"
BASE COUNT          283 a          139 c          236 g          331 t
ORIGIN      
        1 tcaaaataac cgttccttaa attttccggc aggtatggaa tatcggttaa catattgtcg
       61 attgcccata tggagtgaat gttgtgaggt agagggggga gttcactaag aagattccct
      121 attgcgctaa tctcttgtaa agaaaatggt aaaggtggta aaactgccag cccattacat
      181 gatacatcta atgtttccag tagttctggt aaaacaggaa gagaacataa ttgattattt
      241 gagacatgaa gctccttcaa agatatgggg agtgtgggta gtgtgattag ttgattgtgg
      301 gacgcattca ataatttaag tccttgaggc aaagcaggca gttcaataag tctgttatag
      361 ctgacattaa gctgtgtaag ggacgcaggc aatggggaga ttaagcttaa attattttta
      421 cttatattaa ttgatttaat tcccgggggg atttcaggta atgttgtcag gcctaattca
      481 gacaagtcta ggttcgtctc ttggttttgt agacatgata ctagtcgctg aaaagcgatg
      541 tctcgttgtt cttcttgtat gcggttattt ttccattcag tccaatgggt tagataattt
      601 tcatatgcgc ggctagtgtc gattgaataa gtggagaacg aatttgaaat taatctgtga
      661 ttgttatttg tcgggagcat aaatatcagg gcgtattctt tatgttgaga ggtgttggat
      721 tcttttttat ctgctttgca atgttattgg ttcccttcag cagtagacag gaacttctgg
      781 aggaggcgga tatatagttt gtgtaagcat attaactcca tatgttatat attgaagaac
      841 ttctgcttta cactatccta aactggatcg gtttaatgta aaaaaacgat actttttggg
      901 ggagggggca atttttcctt aaggtatgaa aaaaggagct gaagctatat aatagctatt
      961 aatgccactg gtatcagtga atcatgtat
//
LOCUS       DAJTNN010000357          952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTNN010000357 DAJTNN010000000
VERSION     DAJTNN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.368"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..905
                     /locus_tag="OO150_004274"
                     /pseudo
     CDS             1..905
                     /locus_tag="OO150_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          264 a          223 c          234 g          231 t
ORIGIN      
        1 gtcagcttca ctaacagcaa taatccaacc atctctttgt ctgcagtgat ctatttccct
       61 ccaaaatttg atgaaacccg tcagtatcag gcaattgtgg tatctcaccc aggtggggcg
      121 ttaaagaaca aacggccgga acctatgcca aaaaactggc ggaaaaggga tttgtcacta
      181 ttgcttatga cgcatcttat caaggtgaaa gtggcggcga gccgcgtcag ttagaaaacc
      241 catacattcg tactgaagac atcagtgccg tgattgatta cctgacaacg ctttcttatg
      301 tcgacaatac ccgaattggt gcgatgggaa tttgtgccgg tgcaggatat acagccaatg
      361 ctgcaattca ggatcgccgc atcaaggcca tcggtaccgt tagtgcggtc aatattggtt
      421 cgatgttccg taatggttgg gaaaacaatg tgaaatctat tgatgccctg ccatacgttg
      481 aagcgggttc aaacgcccga accagcgata ttagcagcgg agaatatgcc gttatgccac
      541 tggcaccaat gaaggagtcc gatgcgccga acgaagaact gcgtcaggcc tgggagtatt
      601 accacacccc tcgcgcgcag tatccaacag caccgggtta cgctactttg cgcagcctta
      661 accagattat tacctttgat gcttaccata tggcggaagt gtacctgact cagccgatgc
      721 agattgtggc gggcagccag gcagggagca aatggatgag tgacgatctg tacgatcgag
      781 catcaagcca agataaacgc tatcacattg ttgaaggcgc aaaccatatg gatctttatg
      841 acggcaaagc ctatgtggct gaagcaattt cagtattagc tccgttcttt gaggaaactc
      901 tgtaatgaaa catattcaaa tccgcaatag tgatatggcc tggcatattg ct
//
LOCUS       DAJTNN010000358          947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTNN010000358 DAJTNN010000000
VERSION     DAJTNN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.369"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..181
                     /gene="tnpB"
                     /locus_tag="OO150_004275"
     CDS             <1..181
                     /gene="tnpB"
                     /locus_tag="OO150_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5518181.1"
                     /translation="LWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGI
                     AWQHPKRTERPGIRI"
     gene            201..>947
                     /locus_tag="OO150_004276"
     CDS             201..>947
                     /locus_tag="OO150_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5518182.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIP
                     LYRQSEIYARQGVELSRNTMVRWVSEMADKL"
BASE COUNT          262 a          249 c          251 g          185 t
ORIGIN      
        1 actgtgggcc gatcgtgacg ggttatgcct gttcgccaaa cgcctggaac ggggccgctt
       61 cgtctggccg gtgacccggg aagggaaagt gcacctgacg ccagctcagt tatccatgct
      121 actggagggg atcgcgtggc aacatcccaa acggacagaa cggcctggca tccggatata
      181 acccgtgata aaacaaggga atgaacaatg aactccccga tgatattgag ctgcttaaag
      241 ccatgttgcg taagcaacag agtcggcttc gacagtatgc ctgtcaggtc gcgggctatg
      301 agcaggaaat tgaacggctg aaagcgcaac tcgacaggtt gcgtcgtatg ctgttcggcc
      361 agagttcaga gaaaaagcgt cataagcttg aaaatcagat ccgacaggca gaaaaacgac
      421 tgtcggaact ggaaaaccgg ctgaacacag ccagaaatct tctggaagat gcatcgtcag
      481 tcacagattc acctgacacc agtcccccgt cagaaaaccc gatcgccagt aagcctgaat
      541 ccccgggacg aaaatcttca cgaaaaccgc tgccggcaga acttccccgg gagacacatc
      601 gccttctgcc tgctgaaacc agttgcccgg cctgtggagg tgttctgaaa gaaatggggg
      661 aaacaatctc agagcaactg gatatcatta ataccgcctt taaagttatc gaaaccatac
      721 gtcccaaact ggcctgtagc cggtgtgatg tcatcgttca ggcaccactt ccccctaaac
      781 cgatcgaacg cggttatgcc agtgcagggt tacttgcacg gatcctggtc agcaaatata
      841 tggaacatat ccctttatat cgccagtcag aaatatacgc gcgacagggc gtggagctga
      901 gccgtaatac catggtgcgc tgggtatcag aaatggcaga caaactc
//
LOCUS       DAJTNN010000359          945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTNN010000359 DAJTNN010000000
VERSION     DAJTNN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.370"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>945
                     /gene="cusB"
                     /locus_tag="OO150_004277"
     CDS             <1..>945
                     /gene="cusB"
                     /locus_tag="OO150_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR5518183.1"
                     /translation="RKILFWYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVR
                     IDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVG
                     DKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLI
                     ATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWL
                     VKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWL
                     QLNTASEPMLLIPSQGLIDTGSEQRVITIDADGRFVPKR"
BASE COUNT          220 a          259 c          273 g          193 t
ORIGIN      
        1 acgtaaaatc ttattctggt acgacccgat gtaccccaat acgcggttcg ataaaccagg
       61 taaatcgccg tttatggata tggatctggt gccgaaatat gccgatgaag agagttctgc
      121 gtctggtgtg cgcattgacc cgactcagac gcagaatctg ggggtgaaaa cggctaccgt
      181 cacgcgcgga ccgctgactt ttgcccagag tttcccggcg aatgtcagtt acaacgagta
      241 tcagtatgcc attgtgcagg ctcgcgctgc cgggtttatc gacaaggtgt atccgcttac
      301 cgtgggcgat aaagtacaaa agggcacacc gcttctcgac ctgaccattc ctgactgggt
      361 ggaagcgcag agtgagtatt tactgctgcg cgaaaccggc ggtacggcga cccagactga
      421 aggcattctt gagcggctgc gactggcggg aatgccggag gcggatattc gccgcctgat
      481 cgccacgcaa aaaatccaga ctcgctttac gctcaaagcg cccattgatg gcgtgatcac
      541 cgcgtttgat ctgcgcgcgg gaatgaatat cgccaaagat aacgtggtag cgaaaattca
      601 gggtatggac ccggtgtggg tcactgctgc gatcccggag tctatcgcct ggctggtgaa
      661 agatgcctcg cagtttacgc tcaccgttcc ggcacgaccg gataaaacac tcaccatccg
      721 caaatggacg ctgctacccg gcgtggatgc cgcgacccgc acgctgcagc tgcgtctgga
      781 agtcgacaac gccgacgagg cgctaaaacc gggcatgaac gcctggttgc aactcaatac
      841 cgccagcgaa ccgatgctgc tcattccgtc acaagggctg attgataccg gtagcgaaca
      901 gcgggtgatt accattgatg ccgacgggcg ctttgtaccg aaacg
//
LOCUS       DAJTNN010000360          945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTNN010000360 DAJTNN010000000
VERSION     DAJTNN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.371"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            8..571
                     /locus_tag="OO150_004278"
     CDS             8..571
                     /locus_tag="OO150_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5518184.1"
                     /translation="MCLAVSGAPAWASEHQSTLSAGYLQPHTDMPGSDDLKGINVKYR
                     YEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAY
                     AMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESV
                     AIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            635..>945
                     /locus_tag="OO150_004279"
     CDS             635..>945
                     /locus_tag="OO150_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5518185.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     M"
BASE COUNT          204 a          222 c          302 g          217 t
ORIGIN      
        1 cgcagccatc tgtctggctg tatccggtgc gcctgcatgg gcgtctgaac atcagtccac
       61 gctgagcgcc gggtatcttc agccccatac tgatatgcca ggcagcgatg acctgaaggg
      121 cattaacgtg aaataccgtt atgagtttac ggacacgttg gggctggtga cgtcattcag
      181 ctatgccaac gctgaagatg agcaaaaaac gcattacagc gatacccgct ggcatgagga
      241 ttccgtgcgt aaccgctggt tcagcgtgat ggcggggccg tctgtgcgcg tgaatgaatg
      301 gttcagcgcg tatgcgatgg cgggcgtggc ttacagccgt gtgtcgacct tctccgggga
      361 ttatctccgc gtaactgaca acaaggggaa aacgcacgat gtgctgaccg ggagtgatga
      421 caaccgccac agcaacacgt ctctggcgtg gggggctggc gtgcagttta acccgaccga
      481 atccgtggcc attgaccttg cttatgaagg ttccggcagt ggcgactggc gcactgacgg
      541 tttcatcgtg ggtgtcggtt ataagttctg attagccagg taacacagtg ttatgacagc
      601 ccgctggttc aggcgggctt tttgtggggt gaatatggca gtaaagattt caggtgtact
      661 gaaagatggg gcgggtaaac ctgtcgtaaa ttgtgcgatt gaactgcggg ccagaagaac
      721 cagtccgacc gttgtggcac acgttgttgc cacttgcgtg acggacaata acggtgctta
      781 tgtgattgag gctgagccgg ggtattacga ggttgcgctt cactgtaacg gctggcagcc
      841 aacccgtgtc ggggatattg atgtggcacc gactgatgca ccggggacac tgaacgcgtt
      901 tctgaatgca ccaaaggatg gtgatttacg tccggaggtg atgaa
//
LOCUS       DAJTNN010000361          943 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTNN010000361 DAJTNN010000000
VERSION     DAJTNN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.372"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>943
                     /locus_tag="OO150_004280"
     CDS             <1..>943
                     /locus_tag="OO150_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5518186.1"
                     /translation="IAELQSQFAGFGASAKESMGIQGSEELFRNMIGYSRLMGRSEEE
                     IKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKSEQELFDAMKKGALK
                     SADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSENGLQDF
                     TRSLTTLLNSLGGSGKSLGESLGSLMTSMSSGVDSLTDISYRVRAFFDEVTLAYRGLT
                     DEQKKVVDALGSGLINSLKVLAGVIAVNKVVGVTRSIYGLMTAITRLGQVADTTADNV
                     NRKNGKPSRLNPMKMLLAGSAIYAGYERLASTPERMEA"
BASE COUNT          288 a          149 c          241 g          265 t
ORIGIN      
        1 atatcgctga actgcaatca cagtttgctg gttttggtgc ttcagctaaa gagtctatgg
       61 gtattcaagg ttctgaagag ctttttagaa atatgattgg ctattctcgt ttaatgggtc
      121 gaagtgagga agagattaag cgagcactga cagcattatc acagatggct ggtaagggtc
      181 agattatggc tgaagagctt aaggggcaac tagctgaagc tgttcctggt atggttcagg
      241 tattcgctaa agcaaccgga aagagtgaac aggaattatt tgatgccatg aagaaggggg
      301 ctttaaaatc tgccgatact cttcagaagg tcactcagga actgaataaa caaatcacag
      361 ctaaaggtgg ctggaaagct atttcagaaa gcacacaagc acaactgggg aacctgaaaa
      421 actcttggaa taccacgctt gatagtatct tcagaggttc tgaaaatggt ctgcaagatt
      481 ttacacgaag cctaacaacg cttttaaaca gtttaggtgg aagtggtaag agtcttggtg
      541 aatctcttgg tagcttgatg acttctatgt caagtggtgt ggatagcctg accgatatca
      601 gttacagagt gagagcattc tttgatgagg tgacattagc ttatcgtggt ttaaccgatg
      661 aacaaaagaa agttgttgat gctttaggtt ctggcttgat taatagttta aaagtcttgg
      721 ctggtgttat tgctgtgaat aaagttgttg gtgtaaccag aagtatttac gggcttatga
      781 ctgctatcac aagattaggt caggtagctg atactacagc ggataacgtg aatagaaaga
      841 atgggaaacc atcaagacta aaccctatga agatgctatt agctggtagt gctatctatg
      901 caggttatga aagattagct tctacacctg aacgaatgga agc
//
LOCUS       DAJTNN010000362          925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTNN010000362 DAJTNN010000000
VERSION     DAJTNN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.373"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>925
                     /locus_tag="OO150_004281"
     CDS             <1..>925
                     /locus_tag="OO150_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91-like element ISSbo1 family transposase"
                     /protein_id="HCR5518187.1"
                     /translation="RVEAVTKMLACGTRILGVKEYICDKPECPHVRYVTNSCGSRACP
                     SCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLY
                     AARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWM
                     WNMRQLLLKAWSEGLAMPESLSHITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTAR
                     YLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATETLTQRELVARLKQHIPEK
                     FFKMVRYFGFLANRVCGEKLPQVYRALGMDKQ"
BASE COUNT          216 a          232 c          298 g          179 t
ORIGIN      
        1 cagggttgag gctgtcacaa aaatgctggc ctgcggcacg cggatactgg gagtgaagga
       61 atacatctgc gataaacctg agtgccccca cgtcagatac gtcactaact catgcggcag
      121 ccgtgcctgc ccgtcctgcg gaaaaaaggc cacagacctg tggatagcga cacagctgaa
      181 tcgtcttcct gactgcgact gggtacacct ggtcttcacc ctgccggaca cgctgtggcc
      241 ggtgttcgaa agcaaccggt ggctgctgaa tgacgtgtgc cgtctggcgg tggagaatct
      301 gctgtatgcc gcccggaaac gggggcagga acccggtatc ttctgcgcca tccacacgta
      361 tggccgtcgt ctcaactggc atccgcatgt acatgtgtct gtaacctgtg gaggtctgaa
      421 taagcatggt cagtggaaaa agctgagctt cctgaaagac gcgatgcgtt cacggtggat
      481 gtggaatatg cggcagctgc ttctgaaagc gtggtcagag gggctggcga tgccggagtc
      541 gttgtcacat atcacgacgg aatcacagtg gagaagcctg gtgctgaaag ccggaggaaa
      601 atactggcat gtgtacatgt cgaaaaaaac ggccggcggg aggaatacgg cgcgctacct
      661 gggtcgttat ctgaagaagc cgccgatagc ggcctcccgg ctggcacatt acaacggagg
      721 ggcgagcctg agcttccgtt acctggacca caaaacggga gaaacggcga cggaaacgct
      781 gacacagcgt gagctggtcg cgaggctgaa acagcacatc ccggagaagt tttttaagat
      841 ggtgaggtac ttcgggttcc ttgccaaccg ggtgtgtgga gagaagctgc cgcaggtgta
      901 ccgtgctctg gggatggata aacag
//
LOCUS       DAJTNN010000363          914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTNN010000363 DAJTNN010000000
VERSION     DAJTNN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.374"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>914
                     /gene="tuf"
                     /locus_tag="OO150_004282"
     CDS             <1..>914
                     /gene="tuf"
                     /locus_tag="OO150_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5518188.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          212 a          230 c          264 g          208 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccg
//
LOCUS       DAJTNN010000364          909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTNN010000364 DAJTNN010000000
VERSION     DAJTNN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.375"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>909)
                     /locus_tag="OO150_004283"
     CDS             complement(<1..>909)
                     /locus_tag="OO150_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5518189.1"
                     /translation="AFLTGIAGALQTPTLSIFLADELKARPIMVGFFFTGSAIMGILV
                     SQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNRNYFILLSTGVLLSSFASTANPQ
                     MFALAREHADRTGRETVMFSTFLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAF
                     VVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRDTRLLFVVCSMMWAANNLYMINM
                     PLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYYMKRIGKRLLMLIAIVSGMCFYA
                     SVLMATTPAVELELQILNAIFLGILCG"
BASE COUNT          280 a          214 c          213 g          202 t
ORIGIN      
        1 ccacagagaa taccaaggaa gatggcattt agaatttgca attccagctc aaccgccgga
       61 gtcgtcgcca tgagtacgct ggcgtaaaaa cacataccac tcacgatagc aatgagcatt
      121 aatagtcgct tgccaatacg tttcatgtaa tagcctgcga ttaacatcat cggaatttcc
      181 agaccggcag cgataccaat catttctcca gccagtttat cggttagatg cagttcatca
      241 ataataaata gcggcatatt tatcatgtag agattattcg ccgcccacat cattgaacag
      301 accacaaaaa gtagccgcgt atcccgcttc ctatgggtgg agggtaaaat ttctacgggt
      361 tgggtaacga caggaatatt tctttgtatt gatggcaaaa acaaccagac tatcagcccg
      421 caaacaacaa atgcgatggc agcggtgaga tacatcactt taaaactaaa ccccattgcc
      481 agttcataag cgagcggtgg cccgataacc caggcaagcg agatctgagc acgtaaaaat
      541 gtactgaaca tgaccgtctc acggcctgtt ctgtcggcgt gttcacgggc gagggcgaac
      601 atttgcgggt ttgcggtaga agcaaaacta ctcagaagta cgcccgtaga gaggagaatg
      661 aagtagttgc gattccacgc aaaaagcgtg caggccagca ctccaaataa gcaacatagc
      721 agaatcagta atttacggtc gccttgttta tcggagtgcc ttgccagaaa ttgactgacc
      781 agaattccca taatagcgct accggtgaag aaaaaaccta ccattatagg acgggctttc
      841 agttcatctg cgaggaatat acttagggta ggagtctgaa gagcgcccgc aatacccgtc
      901 agaaaggcg
//
LOCUS       DAJTNN010000365          909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTNN010000365 DAJTNN010000000
VERSION     DAJTNN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.376"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..316)
                     /locus_tag="OO150_004284"
     CDS             complement(<1..316)
                     /locus_tag="OO150_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5518190.1"
                     /translation="MKACLLLFFYFSLIDQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNP"
     gene            complement(328..>909)
                     /locus_tag="OO150_004285"
     CDS             complement(328..>909)
                     /locus_tag="OO150_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5518191.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDLIH
                     SVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCN
                     TSKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMA
                     ITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          264 a          191 c          146 g          308 t
ORIGIN      
        1 ttggatttgt atctgataaa aagaccccat aattaaactg cgctgtaaca ttcccctgag
       61 atgctgatct ttccaagaat cgcaattgtt tatcaatatt atttttagta aagcagtaat
      121 attgatatag tcgaaatgat gcctctgaat caccatgttg tgatttatac ttcagtttca
      181 tcagcttttc ttcacttaaa tcataggtca ttgctgtaga tcccatcaca ctttcgtttt
      241 cttttatttt cacatcagca ccatgcaatt gatcaataag agaaaaataa aaaaatagta
      301 acaagcatgc tttcatacat ctacaaccta tcttatatca ctgggttttt ctggcgtacc
      361 ttgcatatta gaaatggggt gcccagatgg cccatatgtt atccaattac caaggtcttt
      421 tgttgttata gccattagat ccttatttga attaaggttc gttggtgctg ttagcactgt
      481 atccattttt aacttaccat tagttcctcg cccccaccac gtccatagcg tgtcaggtcc
      541 tattacattt gtatgtaact ctttagctaa ttggctagca aatgaatttt gtcctttact
      601 tgtattacaa gaaaataacc aaactgtcat cccatcttta taattcccat caaatttaat
      661 tagatatgct agatctttag ctgtcatgat actgcgcgtt gcggattcaa tagatgtggg
      721 ggtaccatgc cccccgattg tgaaaacgcc tgggatatta atctcatcag ctacagaatg
      781 gataagatca ctttcggggt ataaatttat atcaaccaaa ccttgcggat ctacatttat
      841 gaccggattc aacggatact gataaaaatt ccatcccccc ttcagcccaa tcggatcctg
      901 agtgatata
//
LOCUS       DAJTNN010000366          904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTNN010000366 DAJTNN010000000
VERSION     DAJTNN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.377"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..264
                     /locus_tag="OO150_004286"
     CDS             <1..264
                     /locus_tag="OO150_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5518192.1"
                     /translation="SGRALRNELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNV
                     VERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            379..>904
                     /locus_tag="OO150_004287"
     CDS             379..>904
                     /locus_tag="OO150_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR5518193.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEH"
BASE COUNT          242 a          231 c          242 g          189 t
ORIGIN      
        1 tcagggcgcg cattgcgcaa cgagctgaaa aataacggta taaaggcagt aatcccccga
       61 aagtcaaatg agaaaatggc atcggatgga cgtgcacagc ttgatcgtga tgcgtactgc
      121 aatcgtaatg tcgttgaacg gtgctttggg cggctgaaag aatatcgccg catcgccacg
      181 cgttacgaca aaacggcgag aaattacctg gcgatggtga aactgggctg catccgactc
      241 ttttatcaac gcttacgtaa ttaagggaca cagcctaaat agctgcgcgg aatagtagat
      301 ccctttgagg gaacttagcc cggattgtgc gatctgatca atcgccaaat caaaacaaat
      361 caccaaccgg actgagcaat gccgatcata gcaccaattt cccgtgacga acgacgcctg
      421 atgcagaaag ccatccataa aacacacgat aaaaattatg cccgcagact gactgccatg
      481 ctgatgctgc accggggcga ccgtgtcagc gacgttgcca gaacgctctg ctgcgcccgt
      541 tcctctgttg gacgctggat taactggttc acgcagtcgg gtgttgaggg actgaaatca
      601 ttacctgccg ggcgtgcccg tcgctggccg tttgagcata tctgcacact gttacgtgag
      661 ctggtaaaac attctcccgg cgactttggc taccagcgtt cacgctggag tacagaactg
      721 ctggcaataa aaatcaatga gataaccggt tgccagttaa atgccggaac cgttcgccgc
      781 tggttgccgt ctgcggggat tgtgtggcga agggctgcgc caactctgcg tatccgtgac
      841 ccgcataaag atgaaaagat ggcagcaatc cataaagcac tggacgaatg cagcgcagag
      901 catc
//
LOCUS       DAJTNN010000367          903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTNN010000367 DAJTNN010000000
VERSION     DAJTNN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.378"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>903
                     /gene="preT"
                     /locus_tag="OO150_004288"
     CDS             <1..>903
                     /gene="preT"
                     /locus_tag="OO150_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5518194.1"
                     /translation="LQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLT
                     NQGYDVTIYEKEAQPGGWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLT
                     LEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIG
                     GGDVTMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVE
                     GNKVTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVF
                     AAGDIVEGDKTMVYAVKTGKEAAE"
BASE COUNT          241 a          217 c          255 g          190 t
ORIGIN      
        1 gcttacagcg ttttgttact gattttgaac aacaaaccgg gatggaaatt tatcagcccg
       61 gtactaaaac gctcggcaaa gtcgcgatta ttggcgcagg tcctgccgga ttgcaggcca
      121 gtgtgacact gacaaaccag ggttatgacg tcacgatcta tgagaaagaa gcacaacccg
      181 gtggctggtt gcgtaacggc attccgcaat tccggttacc gcaatcagta ctggatgcag
      241 agatcgcccg tattgaaaaa atgggcgtga ccattaagtg caacaacgaa gtcggtaaca
      301 cactcaccct tgagcagctg aaagcagaaa accgcgcggt actggtcacc gtggggttat
      361 caagcggttc cgggctaccg ctgtttgagc atagtgacgt tgagattgcc gtcgacttct
      421 tgcaacgtgc acgacaggcg caaggcgata tcagcattcc acaaagcgca ttaattatcg
      481 gcggcggtga tgtcacgatg gacgtagcca gcacgctgaa agttctcggc tgtcaggcgg
      541 taacttgcgt agcgcgtgaa gagttagatg agtttccggc aagcgaaaaa gagtttacca
      601 gcgcccggga gctgggcgtt tcgatcatcg atggattcac gccagtagcc gtggaaggca
      661 ataaagtcac gtttaagcat gtacggttat cgggcgaact gacgatggcg gcagataaaa
      721 ttattctcgc cgtcggtcag catgccagac tagatgcctt tgcggagtta gagccgcagc
      781 gtaacaccat caaaacacaa aattaccaga cccgcgaccc gcaagtcttt gctgctggcg
      841 atattgttga gggtgacaaa accatggtct atgccgtgaa aaccgggaaa gaagccgccg
      901 agg
//
LOCUS       DAJTNN010000368          892 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTNN010000368 DAJTNN010000000
VERSION     DAJTNN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.379"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>892)
                     /locus_tag="OO150_004289"
     CDS             complement(<1..>892)
                     /locus_tag="OO150_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR5518195.1"
                     /translation="GTKGVNIADRQEMKRLNDYMMTELIGANNNHVVPSTPQSWAEYS
                     DPKSRWTARKGLFWGAAEGGPIETGEIPPGNQNTVGFRTYKSVFDLGPAAEKYTVKMS
                     GCHSCPIRCMTQMNIPRVKEFGVPSTGGNTCVANFVHTTIFPNGPKDFEDKDDGRVIG
                     NLVGLNLFDDYGLWCNYGQLHRDFTYCYSKGVFKCVLPAEEYAEIHWDQLEAGDVNFI
                     KDFYYRLAHRVGELSHLADGSYAIAERWNLGEEYWGYAKNKLWSPFGYPVHHANEASA
                     QVGSIVNCMFNRDCMTHTHI"
BASE COUNT          228 a          244 c          193 g          227 t
ORIGIN      
        1 ttgatatggg tatgtgtcat gcagtcacgg ttgaacatac agttaacaat ggaaccgacc
       61 tgcgctgacg cttcattggc atggtgaacc ggatagccaa acggcgacca gagtttattt
      121 ttcgcgtagc cccagtactc ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca
      181 tcagccaggt gactcagctc acccacacga tgcgccagac ggtagtaaaa atctttaatg
      241 aaattaacgt cacccgcttc cagttgatcc cagtgaattt ccgcatactc ttcagcaggc
      301 agaacacact tgaacacccc tttgctgtaa caataagtaa agtcgcgatg caactgcccg
      361 tagttacacc ataggccgta gtcatcgaac aaattcagac caaccaagtt accaatcaca
      421 cggccatcgt ctttatcttc aaaatctttc ggaccgttcg ggaagatggt ggtatggaca
      481 aagttggcta cacaagtgtt accacctgtg ctgggcacgc caaactcttt cacccgagga
      541 atattcattt gggtcataca acggatcggg caagagtggc agccactcat ttttactgtg
      601 tatttctctg ccgccggacc taagtcaaaa acggatttat aggtacgaaa gccgaccgtg
      661 ttctgattgc ctggcggaat ttcccccgtt tcaatcggac caccttcagc cgcgccccaa
      721 aacagccctt tacgtgctgt ccaacgtgac ttgggatctg aatactctgc ccacgattgc
      781 ggcgtacttg gcacgacatg gttgttattc gcaccaataa gttcagtcat catgtaatcg
      841 ttgagacgct tcatctcctg acgatcggca atgttgaccc ctttcgtccc tt
//
LOCUS       DAJTNN010000369          884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTNN010000369 DAJTNN010000000
VERSION     DAJTNN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.380"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..221)
                     /locus_tag="OO150_004290"
     CDS             complement(<1..221)
                     /locus_tag="OO150_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5518196.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHEN"
     gene            298..705
                     /locus_tag="OO150_004291"
     CDS             298..705
                     /locus_tag="OO150_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5518197.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
BASE COUNT          270 a          219 c          167 g          228 t
ORIGIN      
        1 tgattttcat gaatcacctt cccaggtcgt tttgccttac gatattcgtc ataaactttg
       61 gggtcgtact gaagttcccc gccagatgcc tcttgcaggc gcatcgcgcg accttcagga
      121 accagttccc cccatgcagc aatgcttgcc agcctaactc ctgcggcatt ggcaagcttt
      181 gttttgctgc caaaaaacgc tatagcatca attttcaaca tatcgagctc cttagatttt
      241 cctaaggaaa ctagatcgta gagaaaccta agtcaagaaa gattagaatt ccctaatatg
      301 aaaaacgaaa ccttcggtgc tcgcctctta cataggcgta aaaaattaaa actgtctcag
      361 gccgcattag gtaagctggt caaagtggct cacgtaacaa tttctcaatg ggaaagagat
      421 gaaacacagc cggcggggaa aagattattc gcactgagcc aagcgcttca gtgctcaccg
      481 acttggcttc tttttgggga tgaagataaa caaccaggcg aaccgatccc ggataatcag
      541 ccagccattc tgacagaaga tcaaaaagag ttacttcaac tgttcgacgc actgcctgag
      601 tcagagcaaa aggccctgtt gtcagagatg cgtgctcgag ttgaaaattt caacaaactt
      661 tttgaagaac tactcaaagc tcgcaaaaga agcgcaaaca aataatcccc ttttttctcc
      721 acaccctgta ataaaaagca caaactttca aacacttgtg ttttttacat caaaaaaact
      781 taggtttttt atacataaaa tcttgactcc acgtcttagg ttattctaaa ttccattcat
      841 caagacaccg cacggtgttc tcagcaaaca gttccactac cccg
//
LOCUS       DAJTNN010000370          864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTNN010000370 DAJTNN010000000
VERSION     DAJTNN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.381"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(217..504)
                     /locus_tag="OO150_004292"
     CDS             complement(217..504)
                     /locus_tag="OO150_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5518198.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(579..722)
                     /gene="mgrB"
                     /locus_tag="OO150_004293"
     CDS             complement(579..722)
                     /gene="mgrB"
                     /locus_tag="OO150_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5518199.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
BASE COUNT          272 a          201 c          157 g          234 t
ORIGIN      
        1 aataccacgc ttaatcacaa atccgatgta aacatcctaa taaattaatg aggaaaatat
       61 ctttttctga tatcccgtta cgcaaataaa taatatgata attatttgaa gtcagaccag
      121 agttttaatt tgaagcagtt gcaccatgac agtgcgtata atcaggatga attgagtaac
      181 ccttcaacga cggggttact caagattgag gaaggattat cgttcggcga ccaggcgaat
      241 aatatcttcg tcatcaaccg atttttgact ctcaatggtc tttttatctg ctttctctgg
      301 ttcgtttgct tcgcacaaac gacgtaacag tgcattttga cgtttttgct gatccagcaa
      361 cgcctcaagc agttcaatct gttcgttagt ccgtgaactg gcacgattga taaaaaacca
      421 caagatgagc ccgataagaa gaactaccac cgatacaacc aaagacgcaa tgttcatcac
      481 gcctgaattt acaacttcgt tcatttcacc acctctgagt agaggcgcta ttctaccact
      541 gctggagagg aagaaaatct agtgctgaaa aaatgatatc accacgggat aaactggtta
      601 atggcacaaa ttccgctgaa aaattgtaca tcctgatcgc acatcatgtt gaatacctgc
      661 gcccaaagca gcaagcaagc caacaccacg acaaccagaa cgacccatcg aaactttttc
      721 actccaatct ccgcttcacc taccttatgt cattaaacta gcatgcgtta gttaaacagc
      781 gcctaactat gtcgatattc gtgctttttc ggaatgagta gcttgcttca gcgcatattg
      841 ccgctacgat taagcgaaca taaa
//
LOCUS       DAJTNN010000371          861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTNN010000371 DAJTNN010000000
VERSION     DAJTNN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.382"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            35..313
                     /locus_tag="OO150_004294"
     CDS             35..313
                     /locus_tag="OO150_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5518200.1"
                     /translation="MSDAVYRRLILMKAMSNITDCSVPDINRMLRFMFGKKRRAYVLN
                     NGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            300..>861
                     /locus_tag="OO150_004295"
     CDS             300..>861
                     /locus_tag="OO150_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5518201.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTVRDN
                     LQVYSKEQSDQRYVHREGDKITGELKI"
BASE COUNT          224 a          198 c          241 g          198 t
ORIGIN      
        1 ttacaacgga aaatcggata cccggactgt tgacctgtct gatgctgtat accggcggct
       61 gatactgatg aaagccatgt cgaacattac tgactgttcc gttccggata ttaaccggat
      121 gctgagattt atgttcggaa aaaaacggcg agcttatgtt ctgaataatg gtgggctgag
      181 gatgagttac atctttgagt ccgcgctctc gttggcagaa ctggcgatta tccagtcgtc
      241 gggtgcactg ccgtccccgc cgggtgttta tgtttcagta gttttaaagg agtcccgtaa
      301 tgaaggccag tgataaaccc cgccagctgg cggtcccctt tgcgagtacc ggagataaaa
      361 accgtatccc ggacaaggcg acacagcaga ccagagagag cggtaatgct gcgtatgatt
      421 caggttttcc tccggtgacc atgacagcgg tctcagcggg aggtataccg ccacacggca
      481 aggattttaa cggtctgatg tacgatatta ccgcagcaat acggttcgcc caggctggcg
      541 gtttgtacac gtataatgcc ggttttgcgg gggccattgg tggatatgca aaaggagcca
      601 ttctcgccgg agtcgcaaca acagcggtct ggctgaatac cacagacgat aacctgaccg
      661 atcctgaagg ctccgacagt gcgggctggg taaatcttct tgaggatccg aaaaggatat
      721 tcctgcggca gaagaacaat ctgtcagacc ttcagaataa agggacggta cgggataatc
      781 ttcaggttta cagtaaagag cagtcagatc aacgctatgt tcatcgggaa ggcgataaaa
      841 taaccggaga gctgaaaatc c
//
LOCUS       DAJTNN010000372          855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTNN010000372 DAJTNN010000000
VERSION     DAJTNN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.383"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..831
                     /locus_tag="OO150_004296"
     CDS             <1..831
                     /locus_tag="OO150_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5518202.1"
                     /translation="YIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQCIGV
                     EILLRWNNPRQGRISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHVGIN
                     VAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVDYRIARELHRKNVKLAI
                     DDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIELVAE
                     GVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIMPLR
                     "
BASE COUNT          199 a          218 c          217 g          221 t
ORIGIN      
        1 tacatcgcct ggctggcgac cgcttaccgg atgagctttt cccgcgaaat caatctgggc
       61 ctggcgcaac atgagttcga attgttctgt cagcctttgc ttaatgcgcg cagccagcaa
      121 tgtattggtg tagagatttt gctgcgctgg aacaatccgc gtcagggccg gatttcaccg
      181 gatgtgttta ttcctatcgc ggaagaacat catttaattg tgccactgac ccgctatgtg
      241 atggcagaaa ccattcgtca gcgccatgtt ttcccgatga gtagtcagtt tcatgttggc
      301 attaacgtcg cacccagcca ttttcgccgt ggtgtgctga taaaagatct caatcagtac
      361 tggtttagcg ctcacccgat tcagcaactg atcctcgaaa tcaccgaacg cgatgcctta
      421 ctggatgttg attatcggat tgcccgcgag ctacatcgta aaaacgtcaa actggcgatt
      481 gatgacttcg gcaccggcaa cagttcattt tcctggcttg aaacattacg tcctgacgtg
      541 ctgaaaattg ataagtcatt taccgcagct ataggttctg acgcggttaa ctcgacggtg
      601 accgatatca tcatcgcgct ggggcaaaga ctgaatattg aactggtggc ggagggcgtg
      661 gaaacgcaag aacaggcgaa gtatttgcgc cgtcatggcg tgcatatttt gcaagggtat
      721 ttgtacgcac agccgatgcc gctacgcgat tttcccaaat ggctggcggg cagccaaccg
      781 ccgcccgccc ggcataatgg acatatcacg cccattatgc cgttacgtta acgctgatat
      841 gactttccgt gtcaa
//
LOCUS       DAJTNN010000373          846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTNN010000373 DAJTNN010000000
VERSION     DAJTNN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.384"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..722
                     /gene="istB"
                     /locus_tag="OO150_004297"
     CDS             <1..722
                     /gene="istB"
                     /locus_tag="OO150_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5518203.1"
                     /translation="LISAAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMYTR
                     MAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAM
                     GYEAVRAGIQVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     KEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
     gene            738..>846
                     /locus_tag="OO150_004298"
     CDS             738..>846
                     /locus_tag="OO150_004298"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518204.1"
                     /translation="MGRWWRYKWITFHPSLTQHWLWYAYNTKTGGVLAYT"
BASE COUNT          237 a          209 c          210 g          190 t
ORIGIN      
        1 gccttataag cgcagcgcct gcgctgtcac aacaggcagt agaccaggaa tggagttata
       61 tggacttcct ggagcatctg cttcatgaag aaaaactggc acgtcatcaa cgtaaacagg
      121 cgatgtatac ccgaatggca gccttcccgg cggtgaaaac gttcgaagag tatgacttca
      181 cattcgccac cggagcaccg cagaagcaac tccagtcgtt acgctcactc agcttcatag
      241 aacgtaatga aaatatcgta ttactggggc catcaggtgt ggggaaaacc catctggcaa
      301 tcgcgatggg ctatgaagca gtccgtgcag gtatccaagt tcgcttcaca acagcagcag
      361 atctgttact tcagttatct acggcacaac gtcagggccg ttataaaacg acgcttcagc
      421 gtggagtaat ggccccccgc ctgctcatca ttgatgaaat aggctatctg ccgttcagtc
      481 agaaagaagc aaaactgttc ttccaggtca tcgctaaacg ttatgaaaag agcgcaatga
      541 tcctgacatc caatctaccg ttcgggcagt gggatcaaac gttcgccggt gatgcagcac
      601 tgacctcagc gatgctgggc cgtatcttac accactcaca tgtcgttcaa atcaaaggag
      661 aaagctatcg actcagacag aaacgaaagg ccggggttat agcagaagct aatcctgagt
      721 aaaacggtgg atcaatattg ggccgttggt ggagatataa gtggatcact tttcatccgt
      781 cgttgacaca acactggctc tggtatgcgt acaacactaa aactggcggt gtgttggctt
      841 acactt
//
LOCUS       DAJTNN010000374          836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTNN010000374 DAJTNN010000000
VERSION     DAJTNN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.385"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..601
                     /locus_tag="OO150_004299"
                     /pseudo
     CDS             1..601
                     /locus_tag="OO150_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          208 a          188 c          217 g          223 t
ORIGIN      
        1 cagtgagcaa attgatctta cccagcaata cggtcagtta ccgcgcacat ggcctgcctg
       61 tgtcagaaaa tctgctggag caggattttt acgccagtgg tccgaaccag aagtgggcgg
      121 gagacatcac gtacttacgt acagatgaag gctggctgta tctggctgtg gtcattgacc
      181 tgtggtcatt gacctgtggt cacgtgccgt tattggctgg tccatgtcac cacgcctgac
      241 ggcgcaactg gcctgcgatg ccctgcagat ggcgctgtgg cggcgtaaga gaccccggaa
      301 cgttatcgtt cactcggacc gaggaagtca gtactgttca gcagattatc aggcgctgct
      361 gaagtggcat aatctgcgtg gcagtatgag cgcaaagggc tgctgctacg ataatgcctg
      421 cgtggaaagc ttctttcatt cgctgaaagt ggaatgtctc cacggagaac actttatcag
      481 ccgggaaata atgcgggcaa cagtgtttaa ttatatcgaa tgtgattaca atcgctggcg
      541 acggcacagt tggtgtggcg gcctcagtcc ggaacaattt gaaaaccaga acctcgctta
      601 ggcctgtgtc catattacgt gggtaggatc acttacgccc tgtggttgaa acgtgagcat
      661 attgttttca gatgctgttg tcttgcttca gagtgatctg tcctccggat ttacgcaaag
      721 ttatccacag gcactgtgca tttaaaaaac ttatcttttc ttcttgattt taaaaactca
      781 gatcgtcata tcattatgat aaacataata aaaaactcta aacatgagaa ggtttt
//
LOCUS       DAJTNN010000375          833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTNN010000375 DAJTNN010000000
VERSION     DAJTNN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.386"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            357..>833
                     /locus_tag="OO150_004300"
     CDS             357..>833
                     /locus_tag="OO150_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HCR5518205.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGE"
BASE COUNT          211 a          217 c          206 g          199 t
ORIGIN      
        1 actaacctgt tttttatcat ttcaggagga atgcggccat cgtaatacgt ggtgctgaac
       61 tgtccattct ggctaacagg atagttacca aagttgcatt ccagtatttc accaacctta
      121 ggactgaaat taatcgccac gagtacacct ctccttagta aactctgaaa aagtaataac
      181 acaacgttac gacccgatat tttctaagtc taatggattc acgatgaact ccggtttcgg
      241 tcttccctct ctgatttaaa catccgcagc caaccggtta gcggcttaca cgcggtcaca
      301 ttcaaatgcg attctgctac aatcctcccc ccgttcgaag attgagcaat acacctatgc
      361 gtctaaaccc cggccaacaa caagctgtcg aattcgttac cggcccctgc ctggtgctgg
      421 cgggcgcggg ttccggtaaa actcgtgtga tcaccaataa aatcgcccat ctgatccgcg
      481 gttgcggtta tcaggcgcga catatcgcgg cggtgacctt tactaataag gcggcgcgcg
      541 agatgaaaga gcgtgtcgga cagacgctgg gtcgcaaaga ggcgcgtggg ctgatgatct
      601 caaccttcca cacgctgggg ctggatatca tcaagcgtga gtatgcggcg ctggggatga
      661 aggcgaactt ttcgctgttt gacgataccg atcagctggc gttgctcaaa gagttgaccg
      721 aagggctgat tgaagatgac aaagttctcc tgcaacagtt gatttcgacc atctctaact
      781 ggaagaacga tctcaaaaca ccgtcccagg cggcagccag tgcgattggc gag
//
LOCUS       DAJTNN010000376          831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTNN010000376 DAJTNN010000000
VERSION     DAJTNN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.387"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(23..211)
                     /locus_tag="OO150_004301"
     CDS             complement(23..211)
                     /locus_tag="OO150_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5518206.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            472..807
                     /gene="iraM"
                     /locus_tag="OO150_004302"
     CDS             472..807
                     /gene="iraM"
                     /locus_tag="OO150_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5518207.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          297 a          187 c          129 g          218 t
ORIGIN      
        1 gggccagtac gatgacgtag tatcactccg gctcagttgc accactgacc accacatcac
       61 cttctgctgc aatcgcctgc atcagggtat aaggggttat ggccaccgga ctaccaaacg
      121 gctgccagcc ctctttcagt ttatgtgtca gcttttccgc aagatctgac ggcggcgccg
      181 ccctgacaac atcatagtgt ttaaatgcca tggttctttc caccatctga aaaataattc
      241 tttaaaatac ctgacatgta atacagaaaa aacacaaaac cataccttaa ataaaaacct
      301 gattatcaag cagatatgca tggataaact acaagacgag atataaacca ccctgcattt
      361 aaataaacaa taaacaacat cagaaaaata attctgctct atggtttaca ttcaaaaata
      421 tcatttatac ttttcagaac atcaccagca aggcataaac aaggaaacta aatgaagtgg
      481 attgtgattg atacagttat ccagccatca tgcggaatat ctttttcagt catatggagt
      541 aaaataaaat taataatctg gtatcaatcg gatgctttct tacctcctga aagtatattt
      601 acactgactc acacaggcat catgctcaat aacaaagtgc tacctgtaac catttacaac
      661 gtagtaccat tcaataaaac attctggaat ttaatcaaaa acagccagga atgccctaca
      721 aatacagata acgtattgaa tgaatgcttt aataaccgtt gcactctgca aatatgtcct
      781 tatggactaa aacaacaaag tccataagga gtttactcac atctgacaaa a
//
LOCUS       DAJTNN010000377          823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTNN010000377 DAJTNN010000000
VERSION     DAJTNN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.388"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>823
                     /locus_tag="OO150_004303"
     CDS             <1..>823
                     /locus_tag="OO150_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HCR5518208.1"
                     /translation="QLALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPG
                     YFQITAVPKLAVYDPTVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGH
                     DYYFYVRSVNTVGKSAFVEAVGRASDDAEGYLSFYKGLINNTHLGKELWTQIDNGQLA
                     PDLTEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMK
                     DGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMND"
BASE COUNT          213 a          179 c          247 g          184 t
ORIGIN      
        1 gcagctggcg ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca
       61 gggcgatccg gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga
      121 actgataccg gggtattttc agataacggc ggtcccgaaa ctggctgtat acgacccgac
      181 ggtgcagttt gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac
      241 cagcgcccgc tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc
      301 gggccatgat tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt
      361 ggaggccgtc ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt
      421 gatcaataac acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc
      481 gccggacctg actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac
      541 cgtcaataaa aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt
      601 tgatacaaat aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg
      661 acgcctttat attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag
      721 tcaggtgctg ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa
      781 gccgatgttt gttggtcagg gcgatcagat attcatgaac gac
//
LOCUS       DAJTNN010000378          822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTNN010000378 DAJTNN010000000
VERSION     DAJTNN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.389"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..561
                     /gene="ospC4"
                     /locus_tag="OO150_004304"
     CDS             <1..561
                     /gene="ospC4"
                     /locus_tag="OO150_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5518209.1"
                     /translation="QGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIK
                     LRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQDV
                     AEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDN
                     AMKSKNPKMIDFLLKNGAILGKRFEI"
BASE COUNT          291 a          132 c          160 g          239 t
ORIGIN      
        1 caaggattca gagagttttg ttataacaaa aatattgatc ctgttagtct ggatagaatt
       61 ataaactttg tgtttcagct tgagtatcat ataccaagaa tgctaagtac agataacttc
      121 aaaaaaataa aactcagaga catatctttg gaggatgcga ttaaagcatc taattatgaa
      181 gagattaaca ataaagtgac tgataaaaaa atggctcatc aggcccttgc ttattcttta
      241 ggcaataaaa aagctgatat agcactttat ttactatcta aatttaattt tacaaaacaa
      301 gatgtcgcag agatggagaa aatgaacaac aatagatatt gtaatctgta tgatgttgaa
      361 tacttactta gtaaagatgg tgccaactat aaagtgctag aatattttat caataatgga
      421 ttggttgatg taaacaaaaa atttcaaaaa gcaaatagtg gggacactat gcttgacaat
      481 gcaatgaaaa gcaaaaatcc aaaaatgatt gattttttat taaaaaatgg agcaatatta
      541 ggcaaacgat ttgagatatg acctaaaagc tcggccaaat aataatgtcg tcgattatcg
      601 aagtcatcat gagtgattct gtccccacag tgcagcatca gcatgacagt gaattgtctg
      661 gcgtggtcca tttaatgaga tggagatggt ccctctttcc taaatagctg tgccgaatac
      721 cgctacactt ttgccagccc atgtttgcct ccggggaatg ggctgacggt tccttcaaaa
      781 tccattgcac gcagttatgt cggagcggga acttgactcc tg
//
LOCUS       DAJTNN010000379          800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTNN010000379 DAJTNN010000000
VERSION     DAJTNN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.390"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..234
                     /locus_tag="OO150_004305"
     CDS             <1..234
                     /locus_tag="OO150_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5518210.1"
                     /translation="IGVPVIATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQA
                     IFGTTLAGFSQRSRAAYSGQQMLEEYVNFYQNL"
     gene            209..>800
                     /gene="wcaD"
                     /locus_tag="OO150_004306"
     CDS             209..>800
                     /gene="wcaD"
                     /locus_tag="OO150_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5518211.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIK"
BASE COUNT          194 a          182 c          201 g          223 t
ORIGIN      
        1 attggcgtgc cggtgattgc cacccatagc gacgcggcgc gggaagtgtt gcaaaaatcc
       61 ggcggtaaaa ccgtcagcga agaagaggtg ctgcaactag tgcaattaag caaaccggaa
      121 atcgcgcagg cgatatttgg taccacgttg gccgggttca gccagcgcag ccgcgccgcc
      181 tacagtggac aacagatgct ggaggagtat gtcaacttct atcagaatct gtagctacct
      241 gctgctgccg ctgatctacc tgctggttaa cgtcaaaatc gcccagcttg gtgaaagttt
      301 ccccatcact atcgtcactt ttttacctgt cttgttactg ctgtttttag aacgcatcag
      361 cgttaaaaaa ttgatgattg ccttagggat tggtgcggga ctcacggcgt ttaactacct
      421 gtttggtcag tcgctggatg ccagtaaata cgtcacttca accatgctgt ttgtctatat
      481 tgtgatcatt attggcatgg tgtggagtat tcgttttaaa acaatttcgc cacacaacca
      541 tcgcaagata ttacgtttct tttatctggt ggtagggctg gtggtggcgc tggcggcagt
      601 ggagatggcg caaattatcc tcaccggtgg cagcagtatt atggagtcga tttcgaaata
      661 tctgatttac agcaacagct atgtgctgaa tttcattaaa ttcggcggca agcgcacgac
      721 agcactttat ttcgaaccgg catttttcgc tctggcatta atctcaattt ggctcagcat
      781 caaacagttt ggtatcaaaa
//
LOCUS       DAJTNN010000380          790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTNN010000380 DAJTNN010000000
VERSION     DAJTNN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.391"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..414)
                     /locus_tag="OO150_004307"
                     /pseudo
     CDS             complement(1..414)
                     /locus_tag="OO150_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            337..588
                     /locus_tag="OO150_004308"
     CDS             337..588
                     /locus_tag="OO150_004308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518212.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            complement(608..>790)
                     /locus_tag="OO150_004309"
     CDS             complement(608..>790)
                     /locus_tag="OO150_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5518213.1"
                     /translation="IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
BASE COUNT          210 a          184 c          195 g          201 t
ORIGIN      
        1 aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagggc cagtgttgtt
       61 gcgccttgct gccaacgaag cgccactgct catcaagttc acagataagg gcaacatcag
      121 cgtgagcgac tggcgcagat gttatccgcc ggggcgagag ctttttaatg tccgtatgac
      181 ggtattgatg ccgattttca gtgttctggc ggtataacag ccccctgcgc cgttatgtgc
      241 catttcaacg atctgctcct taacgccgag ttttctggct tcataagagt aagtgagctg
      301 gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac tacggccatg
      361 tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag tcatggaaaa
      421 cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac cttttgccgc
      481 aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg aaaaaaatta
      541 tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taatctaaat agctgcgctt
      601 aataccgcta cacttttgcc agcccatgtt tgcctccggg gaatgggctg acggtttcca
      661 taaaatggcg aacttttttc aacagttgcc acattgagcg gcactgatga ttacgcgtta
      721 ttgtgtcgtg aagtgcctgc catagccgtt caacatgatt cacccatggc gagtaaaccg
      781 gctgataaat
//
LOCUS       DAJTNN010000381          783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTNN010000381 DAJTNN010000000
VERSION     DAJTNN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.392"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(2..313)
                     /locus_tag="OO150_004310"
     CDS             complement(2..313)
                     /locus_tag="OO150_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5518214.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            476..572
                     /gene="ffs"
                     /locus_tag="OO150_004311"
     ncRNA           476..572
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO150_004311"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            692..>783
                     /locus_tag="OO150_004312"
     CDS             692..>783
                     /locus_tag="OO150_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HCR5518215.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLF"
BASE COUNT          170 a          210 c          214 g          189 t
ORIGIN      
        1 gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat accatcacac
       61 cttctgccag taatgcctgt cgctgacgct gtaaatccgg tccggttagc gaaattgtgc
      121 cgtggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga cgctttaaca
      181 caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca tcaccgtaag
      241 tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg ggaaatgaat
      301 cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga aaaacgcccg
      361 agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc gatgggttgc aattagccgg
      421 ggcagcagtg ataatgtgcc tgcgcgttgg ttctcaacgc tctcaatggg ggctctgttg
      481 gttctcccgc aacgctactc tgtttaccag gtcaggtccg gaaggaagca gccaaggcag
      541 atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc tcccaccgtt
      601 tcgtcaaaaa aacccaacat ggctaaactt taaccacaac tgacgtcgca agaattgtct
      661 ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt ttgtggttgc
      721 cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggctg cgccattgtt
      781 taa
//
LOCUS       DAJTNN010000382          776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTNN010000382 DAJTNN010000000
VERSION     DAJTNN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.393"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>776
                     /locus_tag="OO150_004313"
     rRNA            <1..>776
                     /locus_tag="OO150_004313"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          188 a          176 c          250 g          162 t
ORIGIN      
        1 cagccatgcc gcgtgtatga agaaggcctt cgggttgtaa agtactttca gcggggagga
       61 agggagtaaa gttaatacct ttgctcattg acgttacccg cagaagaagc accggctaac
      121 tccgtgccag cagccgcggt aatacggagg gtgcaagcgt taatcggaat tactgggcgt
      181 aaagcgcacg caggcggttt gttaagtcag atgtgaaatc cccgggctca acctgggaac
      241 tgcatctgat actggcaagc ttgagtctcg tagagggggg tagaattcca ggtgtagcgg
      301 tgaaatgcgt agagatctgg aggaataccg gtggcgaagg cggccccctg gacgaagact
      361 gacgctcagg tgcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc
      421 gtaaacgatg tcgacttgga ggttgtgccc ttgaggcgtg gcttccggag ctaacgcgtt
      481 aagtcgaccg cctggggagt acggccgcaa ggttaaaact caaatgaatt gacgggggcc
      541 cgcacaagcg gtggagcatg tggtttaatt cgatgcaacg cgaagaacct tacctggtct
      601 tgacatccac ggaagttttc agagatgaga atgtgccttc gggaaccgtg agacaggtgc
      661 tgcatggctg tcgtcagctc gtgttgtgaa atgttgggtt aagtcccgca acgagcgcaa
      721 cccttatcct ttgttgccag cggtccggcc gggaactcaa aggagactgc cagtga
//
LOCUS       DAJTNN010000383          767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTNN010000383 DAJTNN010000000
VERSION     DAJTNN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.394"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            109..>767
                     /locus_tag="OO150_004314"
     CDS             109..>767
                     /locus_tag="OO150_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCR5518216.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLKSFLLVHGVHY
                     VGRADWGPAHRRWLSKYSFESPWRQLAFDEHRRTIEDRQAQCERLESALKEAVTEWRL
                     YP"
BASE COUNT          184 a          187 c          208 g          188 t
ORIGIN      
        1 gagacagatg ctggccaacg tgggatattt aacgcgggtt aacatcgttg tcagagccgc
       61 atgggtgagc tgttgctaca ccccggtatt tccaccacgg aggccagcat gaacaacaat
      121 aacacattgt acgtcgggtt agatgttcac aaagaatcga ttactgtcgc ttatgctatc
      181 aattcagaac ctgttgaact gatgggtaaa attggcacat cacctactga tattcagaat
      241 ctttgtaaac gtctcaggtc aaagtcatcg caggtcagta tcgtttatga agcggggccc
      301 tgtggttatg gactttatcg tcggctggtg aaatccggtt ttgactgcat ggtctgcgcc
      361 ccctcactta tcccgaaaaa accgggggag cgggttaaaa ccgatcgccg tgatgccatc
      421 agacttgtgc gttcactgcg tgcaggagat ctttctgctg tctacgtacc cggcattgaa
      481 gatgaggcat tccgggattt ggcccgggca tgggcatctg cccgcgatga tttacggcat
      541 gcaaggcaac gtctgaaatc gtttcttctg gttcatgggg tccattatgt cgggcgagca
      601 gattggggcc ctgcacaccg acgctggctc agcaaatact cattcgaaag tccctggcga
      661 caattagctt ttgacgaaca ccgtcgtaca atagaagacc ggcaggcaca atgtgaacgg
      721 ctggaatccg ccctgaagga ggccgttacc gaatggaggc tgtatcc
//
LOCUS       DAJTNN010000384          764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTNN010000384 DAJTNN010000000
VERSION     DAJTNN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.395"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="OO150_004315"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="OO150_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5518217.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFINNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLRNGAVSGKRFGR"
BASE COUNT          279 a           99 c          151 g          235 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattaa taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa gaaatggagc ggtatcaggc aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctgca acaacacatt gcatgtggtg aacg
//
LOCUS       DAJTNN010000385          737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTNN010000385 DAJTNN010000000
VERSION     DAJTNN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.396"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..710)
                     /locus_tag="OO150_004316"
     CDS             complement(<1..710)
                     /locus_tag="OO150_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5518218.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESFECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTET"
BASE COUNT          185 a          171 c          184 g          197 t
ORIGIN      
        1 gtttcggtga attcgacatt gccgaatttg tcgaagtgaa caaccagagt gcgaccatca
       61 cccggaatca tcttgtcagc aggtggggtg ttattctggc gcagttcggc ctccatgcgg
      121 tcgaactcag caatgtaggc ttccttgaat gtggcggctt ttttgcccgt gaagcccatc
      181 accaggaaaa cgaagccgtt tttggtgatt tggtaggcgt tataagaatt gccgcggtgt
      241 tcgaatttaa cccgcgaaaa gttgctggtt aaaaatagtt cagaacattc gaaggattct
      301 attttttgaa caacatggtg atgctgcttg ccaaaaaact cagcaatcga aatagaagta
      361 gtgacggcgc gaccattttc gatggttacg tcagggtgag aaagggtagt agccataatg
      421 acatcctcaa gtgataagtt aattaactca ccagcagagg tgctaatctc atgggtggtg
      481 agacgcacag ggttagcact accggtcact cgagaacccg gccagccttg cggctgcccc
      541 gcacgcctca ccataatttg aatgtggctg tgcattacgc ataaaaaaac cgcttcagcg
      601 cggttatgcg ctcgagtaac tttcggggtg ctaatcccgg cacccgtttt atgaggtgca
      661 ggtgcactat aattccaccc gttctggttt tcaatagcta cattcaacat tttctctttc
      721 ctttcatcac cgaagtg
//
LOCUS       DAJTNN010000386          736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTNN010000386 DAJTNN010000000
VERSION     DAJTNN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.397"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            122..736
                     /locus_tag="OO150_004317"
                     /pseudo
     CDS             122..736
                     /locus_tag="OO150_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          285 a          130 c          116 g          205 t
ORIGIN      
        1 ggctattgtg cgctcataca ctcaaattaa agataggttc taaataaatg agcgtttttt
       61 gatagtctat ttcattaggt aatatatatt tgtaacaaat caatcaaaat ggaataaaat
      121 catgctacca tctatttcaa tcaacaatac cagcgcaact tacccagaat ccattaatga
      181 aaataacaat gatgaagtta atggattagt acaagagctc aaaaaccttt ttaatggtaa
      241 ggaataagca cctgtattaa acatctactt gagcttataa aaaacgccat acgagtaaac
      301 gacgatcctt atagatttaa tattaataat tcctcagtta cttatattga tattggctcc
      361 aatgatacag accatattac tattggtatc gacaaccaag aaccaataga attacctgcg
      421 aactataaag acaaagaact cgtccgtact atcattaatg acaacatagt tgagaagact
      481 catgatatca ataacaagga aatgatcttc agcgcattaa aagaaatata tgatggagct
      541 cctagtttta tcttcgataa gatatcacac aaactcagac atacggtaac ggaatttgat
      601 gagagcggga aaagcgaacc aacggactta tttacctggt acggtaaaga taaaaaaggc
      661 gactctctcg ctattgtaat taaaaataaa aacggaaatg attacttatc cctcggttac
      721 tacgatcagg acgact
//
LOCUS       DAJTNN010000387          723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTNN010000387 DAJTNN010000000
VERSION     DAJTNN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.398"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..630
                     /gene="hscC"
                     /locus_tag="OO150_004318"
     CDS             <1..630
                     /gene="hscC"
                     /locus_tag="OO150_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR5518219.1"
                     /translation="SEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSR
                     VETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDING
                     LLEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKQE
                     ELWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
BASE COUNT          197 a          165 c          195 g          166 t
ORIGIN      
        1 agtgaagata ttgaagaggt aatcctcact gatatttgcc cttactcgtt gggcgttgaa
       61 gttaaccgcc agggcgtttc cggcattttc tcgccgatta ttgaacgaaa caccacagta
      121 ccggtgtcgc gtgtagaaac ttactcgacc atgcacccgg agcaggattc aattacggtt
      181 aacgtctatc agggagaaaa ccacaaagtt aaaaataaca ttctggttga atccttcgat
      241 gtgccgttga agaaaaccgg ggcttatcag tcgattgata ttcgctttag ttatgatatc
      301 aacgggttgc ttgaagttga cgtgcttctg gaagacggca gcgttaagtc cagagtgatt
      361 aaccacagcc cggtaacatt gagcgcgcag cagattgaag agagtcggac gcggttatcc
      421 gcattgaaaa tttatccgcg cgatatgctc atcaatcgca cctttaaagc caaacaggaa
      481 gagttgtggg cgcgagcgct gggtgacgag cgagaagaga ttggccgggt gatcaccgat
      541 tttgatgcgg cgctacagtc aaacgacatg gcccgcgtgg atgaagttcg gcggcgggcg
      601 agcgattatt tagccattga gatcccataa acacggctgc cggatgcggc gtaaacgctg
      661 catccggcat cggcgcaaaa aacacatcgt caactaaaca acatcgcgat tttgccgaga
      721 gca
//
LOCUS       DAJTNN010000388          719 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTNN010000388 DAJTNN010000000
VERSION     DAJTNN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.399"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>719
                     /locus_tag="OO150_004319"
     CDS             <1..>719
                     /locus_tag="OO150_004319"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5518220.1"
                     /translation="NQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPF
                     QPELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLV
                     AVKHVFASLFNGRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFD
                     QVVFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQE
                     HGKQVKIEDVPQEQRDIFSLT"
BASE COUNT          150 a          205 c          198 g          166 t
ORIGIN      
        1 ttaaccagtt tctggaccaa agcggcgtaa accagcgcat ttatgaactg ctggataaaa
       61 cggatattga cgatgttact cagcttgcga aagcgggcgc gcaaatccgc cagtggatta
      121 tcgacactcc cttccagcct gagctggaaa acgccatccg cgaagcctat gctcagcttt
      181 ccgccgatga cgaaaacgcc tcttttgcgg tgcgctcctc cgccaccgca gaagatatgc
      241 cggacgcttc ctttgccggt cagcaggaaa ccttcctcaa cgttcagggg tttgacgccg
      301 ttctcgtggc agtgaaacat gtatttgctt ctctgtttaa cggtcgcgcc atctcttatc
      361 gtgtgcacca gggttacgat caccgtggcg tggcgctctc cgccggtgtt cagcggatgg
      421 tgcgctccga cctcgcatct tctggcgtga tgttctccat tgataccgaa tccggctttg
      481 accaggtggt gtttatcact tccgcatggg gccttggtga gatggtcgtg cagggggcgg
      541 ttaacccgga tgagttttac gtgcataaac cgacactggc ggcgaatcgc ccggctatcg
      601 tgcgccgcac catggggtcg aaaaaaatcc gcatggttta cgcgccgacc caggagcacg
      661 gcaagcaggt taaaatcgaa gacgtaccgc aggaacagcg tgacatcttc tcgctgacc
//
LOCUS       DAJTNN010000389          710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTNN010000389 DAJTNN010000000
VERSION     DAJTNN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.400"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..137
                     /locus_tag="OO150_004320"
     CDS             <1..137
                     /locus_tag="OO150_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5518221.1"
                     /translation="RTGEISMSHKVSVGGGSQVNGALGIGVQNALGGIQLLSGITIQV
                     "
     gene            158..679
                     /locus_tag="OO150_004321"
     CDS             158..679
                     /locus_tag="OO150_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518222.1"
                     /translation="MDVYANGQHVFRFQNGVAIALKNIQAGNAKKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFYSQRNTDGSITFAVNGQVIPLNYGNFDARYKHRT
                     EGVQDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQ
                     KLINGIWYNVASI"
BASE COUNT          196 a          142 c          189 g          183 t
ORIGIN      
        1 tgcggacggg tgaaatatcc atgtcgcata aagtgtctgt tggcggtggt tcgcaggtca
       61 atggtgcgct gggtatcggc gttcagaacg ccctgggggg aattcaattg ctttcgggga
      121 taacgataca ggtataaaac aaaacggcga cggcattctg gatgtttatg cgaatggaca
      181 gcatgtattt cgtttccaga atggtgtggc gatagcgtta aaaaatattc aggccggaaa
      241 tgctaaaaaa ttcacgttat ccagcgccaa caactccacg aaaaatgcag cgttttattt
      301 gtggggtaat ccatccaggc ctgttgttgc agagcttggt gatgattccg gctggcattt
      361 ctacagccag agaaataccg atggtagtat cacattcgct gtaaacggac aggtaattcc
      421 gttaaattac ggaaacttcg atgcccgcta taaacatcga actgaggggg tacaggatgt
      481 gcggtatggt tatgaaatgt attacaaccc tggcagcaac accgtttcat ggacgtttcg
      541 ctcaccttcg ggacacgggt tgtcagggat atcgatatcg gataccggcc gtaactcagc
      601 ggataacgtc aacggtgtgt attaccgacc gctgcaaaaa ctgattaatg gcatctggta
      661 taacgtagcg agtatttaac aatgttgcat ttaaaaaata ttacggcagg
//
LOCUS       DAJTNN010000390          702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTNN010000390 DAJTNN010000000
VERSION     DAJTNN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.401"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..679
                     /locus_tag="OO150_004322"
     CDS             <1..679
                     /locus_tag="OO150_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HCR5518223.1"
                     /translation="TALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIRHNNRNVIF
                     LVPHGWSVDIPAMLLAAQGKKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHAREDGIKP
                     FIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVY
                     DEKKHLLTIEIRPPMDACIASADNKTIARQMNKTVEILVGPHPEQYVWVLKLLKTRKS
                     NEADPYP"
BASE COUNT          216 a          142 c          163 g          181 t
ORIGIN      
        1 cacagcattg caatccatag tgatgatggc agaactggca attcgtggtc cggaaaagtt
       61 ccagaaacga gtgttctgga aaggcctcga aattcttgag gaaattcgac acaataatag
      121 aaatgtgatt tttctggttc cccatggctg gagcgtggat attcctgcaa tgttgctggc
      181 agctcaggga aaaaaaatgg ccgccatgtt tcatcagcaa cgaaatccag tgattgatta
      241 tgtctggaat tcagtacggc gtaaattcgg ggggcgctta catgcccggg aggatgggat
      301 aaaaccattt attcagtcag tacgccaggg atactgggga tattacctcc cagatcagga
      361 tcatggtcct gaatacagtg aatttgctga tttttttgcg acctataaag cgacattacc
      421 aatcattgga cgtctgatga acatcagtca ggctatgatt ataccacttt tcccggttta
      481 tgatgaaaaa aaacatctac tgactattga aattcggcca ccaatggatg cttgcattgc
      541 cagcgcggac aataaaacga ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg
      601 gccacatccg gaacagtatg tctgggtttt aaaattgtta aaaacacgca aatcaaacga
      661 agcggacccg tacccttgaa attataaagt acaagtattt cc
//
LOCUS       DAJTNN010000391          698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTNN010000391 DAJTNN010000000
VERSION     DAJTNN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.402"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>698)
                     /gene="dmsC"
                     /locus_tag="OO150_004323"
     CDS             complement(<1..>698)
                     /gene="dmsC"
                     /locus_tag="OO150_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR5518224.1"
                     /translation="AEAQQRVIACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGA
                     SALSNEIASGSIFFAVGGIGWLLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNS
                     IDTVPTWYSIWTPMGFFLTMFMGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGV
                     VSVMQGAELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPL
                     LSVSFILLLAGELI"
BASE COUNT          203 a          215 c          166 g          114 t
ORIGIN      
        1 cgattaattc ccctgccaga agcagaatga atgatacaga aagtagcgga accgcaggct
       61 gataaccttt tagctgtggt gcaatccaca agcacagggc aacggccaaa agcacgatcc
      121 gccaggacat cagcgcacca tagtccggca ccagcgctgc ggcctgctgc acagaactat
      181 gaatagtcgc cagctctgcg ccctgcatca ctgacaccac gccactcact accagtgcca
      241 gtacagaaat tgctggcagc agacgcatcg cccagccatc gacgccagcc agactcaaca
      301 acaggtaacc cagtaacggg ccgcccataa acatcgtcag gaagaagccc atcggcgtcc
      361 agatgctgta ccaggtcgga acggtatcaa tgctgttata cacacgcacc atcatccaga
      421 caaagatgac gccaagaacc atcgtcacta tcagccacag cgtacgcaat gccggtgaca
      481 gctttttcag cattgccagc aaccagccga tgccgcctac ggcaaaaaag atcgaaccgc
      541 tggcgatttc gttactgagt gctgaagccc ctacccggtt gagcgagtta aaagcgcgca
      601 ttggtgaacc aagatggagc atagaggcga taaagccaat gcccatcagc acccataaac
      661 caaacatgca ggcgataaca cgctgctggg cttctgct
//
LOCUS       DAJTNN010000392          680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTNN010000392 DAJTNN010000000
VERSION     DAJTNN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.403"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>680)
                     /locus_tag="OO150_004324"
     CDS             complement(<1..>680)
                     /locus_tag="OO150_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5518225.1"
                     /translation="VGCVLTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGF
                     WWVTFFLPILMAIGMGTVFFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYS
                     LVMSFLWSNTSFGIHRFKVKLDTAYCIKYAILAFLALLPFLAVAGYIIFEQILNAYDS
                     SVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRI
                     RFRSTLTY"
BASE COUNT          263 a          145 c          103 g          169 t
ORIGIN      
        1 gtacgttaaa gttgagcgaa aacgaatacg cccatcattc agtgacaggt tactcataaa
       61 atgatttcgc aacgacaccg ttaaataact ggtgctaaca gcaatcccaa aataatagat
      121 taactgcgcg attatcattt tacgttgcat ttccataaat tgctgtaaat tctcaatatc
      181 atcatttgca tacacagaac tatcatacgc atttaatatt tgttcgaaga taatataacc
      241 agcaacagcg agaaaaggta ataaagctaa aaatgcgagg atggcatatt ttatacaata
      301 cgcagtatct aatttcacct tgaaacgatg tataccgaaa ctggtattgc tccagagaaa
      361 actcattacc aggctatata aagtaccatt aaaaatacca atggaaacaa taccaactat
      421 tgccatcaga accacagata taataacact acttgaacta ttggcatgta gcatctttgt
      481 cgagataaag aaaacagtcc ccatcccaat ggccattaaa atcggcaaga aaaaggttac
      541 ccaccagaac cctttcatag agcaattaaa actaaatctt acgccgttga gactagtcat
      601 caaggcctga taacgcagtc cttttgctgc cataaatata agcaaaacca acagtgacaa
      661 agtcaaaaca cagccaacaa
//
LOCUS       DAJTNN010000393          675 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTNN010000393 DAJTNN010000000
VERSION     DAJTNN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.404"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            193..462
                     /locus_tag="OO150_004325"
     CDS             193..462
                     /locus_tag="OO150_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518226.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..675
                     /locus_tag="OO150_004326"
                     /pseudo
     CDS             459..675
                     /locus_tag="OO150_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          170 a          154 c          197 g          154 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaa
//
LOCUS       DAJTNN010000394          633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTNN010000394 DAJTNN010000000
VERSION     DAJTNN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.405"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>633)
                     /gene="ipaH3"
                     /locus_tag="OO150_004327"
     CDS             complement(<1..>633)
                     /gene="ipaH3"
                     /locus_tag="OO150_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5518227.1"
                     /translation="RNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQ
                     NALISLPELPASLEYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINA
                     DNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLPESLPAVPVRNHHS
                     EETEIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          163 a          107 c          176 g          187 t
ORIGIN      
        1 gggcggtttg ttgcgacaga gactccctga tccgtgagga cagaggattg tcttcgagga
       61 tgatagtgca ggtcggatca aggctaagta tattttccgg aatgtgtgtg atgcgattct
      121 cgcggcaccg gaaaaatatc tcggtttcct ctgagtgatg atttcttaca ggtacggctg
      181 gtaggctttc cggaagatta gtacttacat cgagcgcttc cagtgattca ggtaactcag
      241 ggagaaatgt cagctggtta tttcttactg agagcacttc cagcgatgta ggtaattcag
      301 gaagcatggt tagctgattg ttatctgcat taatatattc cagcaatgca ggcaattcag
      361 gaagcatggt tagttggttg ttatctacat caagatgttt cagagatgcg ggtaattcag
      421 gaagtgttga caggtgattg tcacaggcgt caaggtattc cagcgatgct ggcaattctg
      481 gtaatgatat tagggcattc tgagtaattt ccagaacagt gatttgaggt ggtaagttgt
      541 caggtagcga ggacagattt aaacgattca gttgaagctc actgaactga ttgatgagac
      601 attctttaag tagggagacc gcttcattcc gat
//
LOCUS       DAJTNN010000395          624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTNN010000395 DAJTNN010000000
VERSION     DAJTNN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.406"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>624
                     /locus_tag="OO150_004328"
     CDS             <1..>624
                     /locus_tag="OO150_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR5518228.1"
                     /translation="MTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVTSGHPVNPLLGA
                     KVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQLRDNTLIL
                     SDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELRLSPH
                     RYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRT"
BASE COUNT          102 a          196 c          211 g          115 t
ORIGIN      
        1 atgacgcagt atggcggtag cattgttcag ggttcagccg gggtgcgcat tggtgccccc
       61 accggcgtgg cctgttcggt gtgccccggc ggagtgacgt ccggccatcc ggtcaatccc
      121 ctgctcggtg caaaggtcct tcccggtgaa accgacatcg ccctgcccgg cccgctgccg
      181 ttcatcctct cccgcaccta cagcagttac cggacaaaaa cgcccgcgcc ggtggggagc
      241 ctcggccccg gctggaaaat gcctgcggat atccgcttac agctgcgcga taacacactg
      301 atactcagtg ataacggcgg cagaagcctg tattttgagc acctgtttcc cggtgaggac
      361 ggttacagcc gcagcgagtc actgtggctg gtgcgcggcg gcgtggcgaa actggatgaa
      421 ggtcaccggc tggccgcact ctggcaggcg ctgccggaag aactccgctt aagtccgcat
      481 cgttatctgg cgacaaacag tccgcagggg ccgtggtggc tgctcggctg gtgtgagcgg
      541 gtgccggaag cggatgaggt gctgcctgcg ccgctgccgc cgtaccgggt actgaccggg
      601 ctggtggacc gcttcgggcg caca
//
LOCUS       DAJTNN010000396          624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTNN010000396 DAJTNN010000000
VERSION     DAJTNN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.407"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..90)
                     /locus_tag="OO150_004329"
     CDS             complement(<1..90)
                     /locus_tag="OO150_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5518229.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFR"
     gene            complement(103..>624)
                     /locus_tag="OO150_004330"
     CDS             complement(103..>624)
                     /locus_tag="OO150_004330"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="catalyzes the formation of
                     2-hydroxy-3-oxopropanoate (tartronate semialdehyde) from
                     two molecules of glyoxylate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5518230.1"
                     /translation="LGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFNI
                     PYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKA
                     IRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNA
                     VDAPTETCFMHYE"
BASE COUNT          170 a          167 c          137 g          150 t
ORIGIN      
        1 gcgaaaacca cactgcgcag ctttctcaaa acgggcgaga aaatcatatt caccaaataa
       61 catcgataaa ttagcagaga aacgtaacat tatttatctc ccttattcat agtgcatgaa
      121 gcaggtttca gtcggtgcgt ccactgcgtt atcggcgata tcttcaaatt ccatgacgtt
      181 atccagttcg ctgcccatcg aaatattggt cacacgctcg agaataactt ccacgactac
      241 cggtacccga tattgcgcca ttaaggcttt cgcctgttca aaggctggcg caatatcttc
      301 cggtttgaag acgcgaatcg ctttacaacc taaaccttcc gctactttta cgtggtcgac
      361 gccgtaaccg ttcacttcgc tggagttgat attctcgaaa gcgagttgca cgcagtagtc
      421 catgtcaaag gcgcgttgtg actgacgaat caggccgaga taggcgttat tcaccagcac
      481 atggatgtac ggaatgttga actgcgcgcc aacggctaac tcttcaatca ggaactggaa
      541 gtcaaagtcg ccagaaatcg ccaccacatt gcgtttcgga tcagcggcac aaacccctag
      601 cgcagccgga atcgtccagc ctaa
//
LOCUS       DAJTNN010000397          620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTNN010000397 DAJTNN010000000
VERSION     DAJTNN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.408"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            198..581
                     /gene="panM"
                     /locus_tag="OO150_004331"
     CDS             198..581
                     /gene="panM"
                     /locus_tag="OO150_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5518231.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT          162 a          135 c          163 g          160 t
ORIGIN      
        1 ataacggcac aaagggcgga ataattcact accattcagg ggattatgct gggtattttt
       61 cattctctaa tgttttaact ttgtaattat tgctgttaaa aaattaatca cctgccaaaa
      121 gaaataaaaa agagaaagcc tccgattaaa ttatttcgct acactggttc cacttttgtg
      181 atttacacgg gttacccatg aagctgacca tcattcgatt agaaaacttt agcgaccagg
      241 accggattga cctgcaaaag atctggccgg agtattcccc ttcctcttta caggttgacg
      301 ataaccaccg tatctacgcc gcgcgtttta acgagcgcct gctcgctgcc gtacgggtaa
      361 ccttaagcgg caccgaggga gcactggatt ccctgcgtgt gcgggaagtc acccgacgtc
      421 gcggtgtggg gcaatatctg ctggaagagg ttttgcgtaa caatcctggc gtttcatgct
      481 ggtggatggc ggatgcaggc gtggaagatc gcggtgtgat gacggcgttt atgcaggcac
      541 tgggatttac ggcgcaacag ggcggctggg agaagcgtta atcgtcaagt tgatttcaaa
      601 agtgatgacc aacgagaaat
//
LOCUS       DAJTNN010000398          620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTNN010000398 DAJTNN010000000
VERSION     DAJTNN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.409"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..125
                     /locus_tag="OO150_004332"
     CDS             <1..125
                     /locus_tag="OO150_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5518232.1"
                     /translation="SPSQWAAIGVLGSLLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            130..480
                     /locus_tag="OO150_004333"
     CDS             130..480
                     /locus_tag="OO150_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5518233.1"
                     /translation="MTQNYKLIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            544..>620
                     /locus_tag="OO150_004334"
     CDS             544..>620
                     /locus_tag="OO150_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5518234.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPD"
BASE COUNT          167 a          136 c          172 g          145 t
ORIGIN      
        1 tttccccgtc tcagtgggcg gcaataggcg tgctggggag tctgctgttt gggctgctga
       61 catatctgac taacctgtat ttcaaaatca gagaggaccg tcgtaaggct gcacggggag
      121 agtaattcaa tgactcaaaa ctataaactg attgtgaaag ggatccgcaa ttttgagaat
      181 aaagttacgg taactttagc gttacgggac aaaaaacgct ttgacggtga aatttttgac
      241 ctggacatct cgctggaccg tgttgaaggt gccgcgctgg agttttatga ggcagcagcc
      301 agaaggagca tcagacaggt cttcctggat gttgctgccg ggttatgtga aggggatgag
      361 cagtcgccgg aaaagcgccc cgtaatttta gaggcgcaga atgtatggat aacctacaaa
      421 ggaaagctac cgggaagaat tactggttct ctgaagactc ctccggaatc acaaccttaa
      481 gtcactgacc ggaacagata aacctgtccg tgggcagaaa ccgataaatc ctgataaata
      541 tccatgaacg caaaaatcag atacggcctg tcggctgccg ttctggcgct gattgccgct
      601 ggtgcgcctg cgcctgacat
//
LOCUS       DAJTNN010000399          590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTNN010000399 DAJTNN010000000
VERSION     DAJTNN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.410"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            325..>590
                     /locus_tag="OO150_004335"
     CDS             325..>590
                     /locus_tag="OO150_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518235.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLS"
BASE COUNT          225 a           99 c          104 g          162 t
ORIGIN      
        1 gactctatca tcgccaatca tcagacattg aaatagacga cactgagatg acgcttacaa
       61 ttcttttggt ttctgtaata agtaatctga catcccccgt gcccagaaga ggagagggat
      121 atcaagccca agggttaata attgatacta cggttaaaat tgccttcagt aaaggaaacg
      181 tgattaaatt caggggattt cagagtgaaa accatacaac atattaaaat ctgttcattt
      241 agctataata catacaagaa agtgtccagt tagataatgt tatctaaata accacagata
      301 aaaacgcaca taattggggg ggggatgaaa atacctgaag cagtaaatca tattaatgtt
      361 cagaataata ttgatcttgt tgatggaaaa acgaatccga ataaagcaac aaaagcatta
      421 caaaaaaata tattgcgcgt aacaaactca tcttcttctg gcataagtga aaaacattta
      481 gatcactgtg ctaatactgt gaaaaacttt ttaaggaagt ctatagcagc gcaaagttat
      541 agcaaaatgt tttctcaagg gaccagtttc aaatcactaa atctttctat
//
LOCUS       DAJTNN010000400          579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTNN010000400 DAJTNN010000000
VERSION     DAJTNN010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.411"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..447
                     /locus_tag="OO150_004336"
     CDS             <1..447
                     /locus_tag="OO150_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518236.1"
                     /translation="LPISAADKRRFYYYFQEKNTPNIERFFVFDSSEYRYALNMREVV
                     FHQFLSDGLRPIVDEDDYFNVHITLVNGGPVIPLSVEPDAPQNEETDDIGQLNAFFDA
                     LDCEPETTDRFMITDEDGEDAFIRIGSIAMVRVALDVLEPVEDDDE"
BASE COUNT          154 a          106 c          142 g          177 t
ORIGIN      
        1 ttgcccattt cagcagcgga taagcggagg ttttattatt attttcagga aaaaaacacc
       61 ccaaacatag aacgcttctt cgtttttgat tcttccgagt atcgttatgc gttaaatatg
      121 cgtgaagtgg tatttcatca gttcctttct gatggtctgc ggccaatcgt tgacgaagac
      181 gattatttta atgtccatat cacgctggta aacggcggac cagtgattcc actgagtgtt
      241 gagccagatg cgccgcaaaa cgaagagacg gatgatatcg gccagttgaa tgcatttttt
      301 gacgcgctgg attgcgagcc tgaaaccacg gatcgtttta tgataaccga cgaagatgga
      361 gaagatgctt tcattcgtat tggttctatc gctatggtgc gagttgcgct tgatgtgctg
      421 gaaccagtgg aagacgatga tgagtagtat tgaatggatt gatttcttaa gaaatggtac
      481 tgagtataca tgtgagtata caaagtaact tcgtattttt aaaaatccat tgatttcata
      541 ttgttgagtc tggaatcgcg gtgcggcctt cgcaccaca
//
LOCUS       DAJTNN010000401          571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTNN010000401 DAJTNN010000000
VERSION     DAJTNN010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.412"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..571
                     /locus_tag="OO150_004337"
                     /pseudo
     CDS             1..571
                     /locus_tag="OO150_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          128 a          140 c          179 g          124 t
ORIGIN      
        1 ctcctcaaag aagccgttga atatggacgg gcaaaaaagt ggatagcgca cgcgccctta
       61 ttgcccgggg atggggagta agcttagtca gccgttgtct ccgggtgtcg cgtgcgcagt
      121 tgcacgtcat tctcagacga accgatgact ggatggatgg ccgccgcagt cgtcacactg
      181 atgatacgga tgtgcttctc cgtatacacc atgttatcgg agagctgccc acgtatggtt
      241 atcgtcgggt atgggcgctg cttcgcagac aggcagaact tgatggtatg cctgcgatca
      301 atgccaaacg tgtttaccgg atcatgcgcc agaatgcgct gttgcttgag cgaaaacctg
      361 ctgtaccgcc atcgaaacgg gcacatacag gcagagtggc cgtgaaagaa agcaatcagc
      421 gatggtgctc tgacgggttc gagttctgct gtgataacgg agagagactg cgtgtcacgt
      481 tcgcgctgga ctgctgtgat cgtgaggcac tgcactgggc ggtgactacc ggcggcttca
      541 acagtgaaac agtacaggac gtcatgctgg g
//
LOCUS       DAJTNN010000402          561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTNN010000402 DAJTNN010000000
VERSION     DAJTNN010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.413"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            87..>561
                     /locus_tag="OO150_004338"
     CDS             87..>561
                     /locus_tag="OO150_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR5518237.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPANAPSWCSDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     EAVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNER"
BASE COUNT          131 a          153 c          153 g          124 t
ORIGIN      
        1 tgcgcactta tacaatctct actccgtttc gattgtgtgc ggtctgcgac gctaaaagaa
       61 aacggcaaaa aggtattacg gcagaaatgg cgatttatca tctcagcatg aaaatcattt
      121 cgcgaaaaaa cggctacagt gccgttgctt ctgctgccta ccgttccggc tctgtcatac
      181 ccgatgaccg taccggatta acccacgatt acacccgtaa acgcggcgtt gatgatgcgg
      241 tcattctcac ccctgcgaat gcaccgtcct ggtgtagtga ccgttccgtt ctctggaatg
      301 cggtcgagaa agccgaacag cgccggaact cccagctggc aagggagatt gaactcgcca
      361 ttccccgtga gatttcccgc gaggccgcac gggaggccgt tctcgctttt gtccgggaaa
      421 actttgtcag tcggggcatg attgccgatg tggcgtttca tcacatggac cggaccaatc
      481 cccatgcgca catcatgctg accacgagag ctgtcgggga aacaggattt gcaggaaagg
      541 tcagggactg gaacgagcgg g
//
LOCUS       DAJTNN010000403          547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTNN010000403 DAJTNN010000000
VERSION     DAJTNN010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.414"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>547
                     /locus_tag="OO150_004339"
     CDS             <1..>547
                     /locus_tag="OO150_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5518238.1"
                     /translation="HPETEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWL
                     AGMKLGDTPLVDFTRDRLHRETLRRFGRYELSTAYTPAGQLQSQHLNSLQYDRDYTWN
                     DNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPD
                     STLSMWPDNRIARDAHYLYRYD"
BASE COUNT          120 a          198 c          150 g           79 t
ORIGIN      
        1 cacccggaga cggaagcact gctctggcag catgagacca gacatgcgta caacgcgcag
       61 ggactggcga accgctgtat accggacagc ctgcccgccg tggaatggct gacctacggc
      121 agcggctggc tggcaggtat gaagctcggc gacacaccgc tggtggattt cacccgcgac
      181 cgcctgcacc gggaaacgct gcgccgcttc ggccgttatg aactctccac cgcttacacc
      241 cctgccgggc agttacagag ccagcacctg aacagcctgc agtatgaccg cgattacacc
      301 tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg gagttacagc
      361 tacagcacca ccggcaggct gaccggcgtt cacaccaccg cagcgaacct tgatatccgc
      421 atcccgtatg ccacagaccc ggcaggtaac cgcctgcccg acccggagct gcacccggac
      481 agcaccctca gcatgtggcc ggataaccgt atcgcccgtg acgcgcacta tctttaccgg
      541 tatgacc
//
LOCUS       DAJTNN010000404          546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTNN010000404 DAJTNN010000000
VERSION     DAJTNN010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.415"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..209
                     /locus_tag="OO150_004340"
                     /pseudo
     CDS             1..209
                     /locus_tag="OO150_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
     gene            191..546
                     /locus_tag="OO150_004341"
                     /pseudo
     CDS             191..546
                     /locus_tag="OO150_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT          127 a          129 c          176 g          114 t
ORIGIN      
        1 gcgagctggc taccgtgaat cctggtaccg atctcaccgt cgttgatgcc agtggcaaac
       61 agccgatcgt tctgttgcag ggttatcaaa tgcagggcag tgaaaacacg ctctacctgg
      121 cggcaggtca acggctggcg ctagccacat taagtgaaga aggtatcaag gcgctcacgc
      181 taaacgggga atggcaggct ggggaattca gcgccagcaa tatggtgaac agaaacactg
      241 gatgctggtg acgctggcgg cggtgttagg gcagattggt acaccgggcg gcggttttgg
      301 tttctcttat cattactcca atggtggcaa cccgacacgt gttggcggcg tgctgccgga
      361 aatgtctgcg gcgattgccg gacaggccag cgaggccgct gatgatggcg gaatgagggc
      421 gattcctgtt gcccgtatcg tcgatgcgct ggaaaatcct ggcggcaaat atcagcataa
      481 cggtaaagag caaacgtatc cgaatatcaa aatgatctgg tgggcgggtg gcggtaactt
      541 taccca
//
LOCUS       DAJTNN010000405          545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTNN010000405 DAJTNN010000000
VERSION     DAJTNN010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.416"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(110..>545)
                     /gene="trpA"
                     /locus_tag="OO150_004342"
     CDS             complement(110..>545)
                     /gene="trpA"
                     /locus_tag="OO150_004342"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5518239.1"
                     /translation="SVLVADVPVEESAPFRQAALRHNVAPIFICPPNADDDLLRQIAS
                     YGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPDQVKAAIDA
                     GAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT          137 a          136 c          150 g          122 t
ORIGIN      
        1 gacaggggta agcgaagcgg taaaaagata aatattaaat gaatttagga tttttccggc
       61 ttcattaaag aaagttaaaa tgccgccaac ggaactggcg gctgtgggat taactgcgcg
      121 tcgccgcttt catcggttgt acaaaaactt tcagtgccgc cagcattttc tctggctcat
      181 taatatgttg ctcgatgatt ttaacaatgg ccgaaccaga aatcgcgccc gcagctcctg
      241 catcaatcgc tgcttttacc tgatccgggg cggaaatacc aaacccctgc aatggaggtg
      301 cagcgttgta ctctttcagc ttcgcaacca gatgattgag gggtaacgcg gcgcggtttt
      361 ctgcgccggt cacgcccgct cgtgacagca aataggtgta accacgaccg taagaggcta
      421 tctggcgcag caggtcgtca tcggcatttg gcgggcagat gaagataggt gcgacattat
      481 ggcgcaacgc ggcctggcgg aagggcgcgg actcttcaac tggcacatcg gcaaccagca
      541 ccgaa
//
LOCUS       DAJTNN010000406          526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTNN010000406 DAJTNN010000000
VERSION     DAJTNN010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.417"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(123..503)
                     /locus_tag="OO150_004343"
     CDS             complement(123..503)
                     /locus_tag="OO150_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003860136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518240.1"
                     /translation="MKAVSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLK
                     EPEEIKLAMQVVVLRDKLEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELR
                     LNRLTMLEKLRELFLRVADISLLQ"
BASE COUNT          144 a          137 c          114 g          131 t
ORIGIN      
        1 aatctcataa aattaatata tgagataatc tttattcagc agaagattat taaaggttgc
       61 tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta ataacggcgt
      121 tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag tttctccagc
      181 atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac tttatcgaag
      241 aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg gtaacgacct
      301 tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc cagtttaatt
      361 tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc gtcagatttc
      421 gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc atccagggta
      481 cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggac
//
LOCUS       DAJTNN010000407          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTNN010000407 DAJTNN010000000
VERSION     DAJTNN010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.418"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            245..525
                     /locus_tag="OO150_004344"
                     /pseudo
     CDS             245..525
                     /locus_tag="OO150_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          137 a          140 c          126 g          122 t
ORIGIN      
        1 caaatccaca tccccgccag cccggctaag atggaggcac ggcacagccc gcacacgacg
       61 ctgaggctac agagctgaaa acccacaagg ccatgccccg catggccggg taaagcggag
      121 ccggcggcga cttcctttgt gctctgtccg ggaccggcag gaaaaaggcg gggataatga
      181 gatggtcacc cccaccctaa cagcagtacg ctgttaaggt gaatccctta acggagagac
      241 catcatacag aacatcacac ttattggtat cgatctcgga aagcattcct tccacattca
      301 ttgtcaagac aagtctggta aggctcttct gcgtaaaaaa tttacccagt actaaactga
      361 tgcagtttct ggcaacatgt ccgccctcag ttgttgtaat ggaagcctgt gcgggtgcac
      421 actttttggc tcgccgtatc tctgaatttt ggacatgaaa ctaagctgat ttctccacag
      481 ttcgttcgcc cgtttgtcaa aagtaataag aatgattttg ttgat
//
LOCUS       DAJTNN010000408          524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTNN010000408 DAJTNN010000000
VERSION     DAJTNN010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.419"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(317..472)
                     /locus_tag="OO150_004345"
     CDS             complement(317..472)
                     /locus_tag="OO150_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518241.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT          168 a           85 c           94 g          177 t
ORIGIN      
        1 ttcgaaacag aaataccagc cctagctaaa gcgtccatgt agagtaatga cgaccgggag
       61 atctgcatca tcaaggtaat ggttcgcttt cgcgaatgcg tcgtgataag taatcataat
      121 tataataaat attattgttg agtgttatat tattattcct gtgtgataca ttgagcaaag
      181 acgcgttcat cttcgtcaaa gatattatta aatgctggtt ttgaaaaaag ttcatcggat
      241 aagatatagt cacgaaataa gtatctgcta atattttatg atttcttttt cagaacgtcg
      301 aacgggattt tcttatttaa gatatcagaa tcttatgatt cgggtaaaat tctaaataaa
      361 tcaacatgtc attaaaatca tcatagccat tagctatttc ggctaaaata gagactacat
      421 gtcttcggtc catctcactt aaggagtgta gttccgttgt aagtttttcc atagcttgca
      481 ctgctaaatt tcgaacaagg aattttctgc tggtaatctc taaa
//
LOCUS       DAJTNN010000409          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTNN010000409 DAJTNN010000000
VERSION     DAJTNN010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.420"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(149..>516)
                     /locus_tag="OO150_004346"
     CDS             complement(149..>516)
                     /locus_tag="OO150_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HCR5518242.1"
                     /translation="DVALPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLG
                     EFGNYSNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLTVGLNALTLTDITKNA
                     TYGVEIESLVLEINAPASS"
BASE COUNT          114 a          147 c          116 g          139 t
ORIGIN      
        1 ccccgtctcc cgttatccgg aagctgacaa caataaaaaa gccaccagtt aagtactgat
       61 ggctctgata actcatgcag gcgtctcaga cgacccactg acactaccgg tgagtttaac
      121 gataccttcc atttgactgg ctcacttttt atgatgatgc cggtgcattt atctccagca
      181 ccagactttc tatctcaacg ccatacgttg catttttggt aatatccgtc agcgtcagtg
      241 catttagtcc cactgtcaga ctgtctttta tgacctggaa tgccgggcca gccactccat
      301 tcagtttcgg agtaaccgtg gcactgccgg cggtgaacac cagctccagc gtctgccagt
      361 cgttactgta attcccgaac tcgcccaact ttgtgtttcc ggctttcttg tgatgcatca
      421 gattcagttt gccgtctgtg gtctgggtga agaacgacat caggaacggg ttaccagtcc
      481 cggtcatcgc cacgacgtca ggtaacgcta catcgg
//
LOCUS       DAJTNN010000410          515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTNN010000410 DAJTNN010000000
VERSION     DAJTNN010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.421"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..280
                     /locus_tag="OO150_004347"
     CDS             <1..280
                     /locus_tag="OO150_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5518243.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          125 a          115 c          139 g          136 t
ORIGIN      
        1 tagcctgaac gtgtactgtc gtaatggcca ggtggaaatc gacaacaaca tcggtgaaaa
       61 cgcgctgcga tcggtggcgg ttggaagaaa aaattatctc ttcttcggtt ctgacaacgg
      121 tggagaaagc gcggcgatta tctacagtct gctggtcacc tgcaaactga acggagtgga
      181 gccggaggac tggttgcgcg aagtgatcgt gaagctcaat gactggccgt cgaaccgagt
      241 gcatgaactg ctgccctgga acttctcagc tgtaaaataa tccttccgct acgtacttct
      301 ctgggcgctt accactcaac ggcatcgtca aaattatcaa agcgcagaac atagatttat
      361 catgtgtttt atggatggct ttctccatca ggcatcgttc gtcacgggaa attggtgcta
      421 tgatcggcat tgatcagtcc ggttggtgat ttgttttgat ttggcgattg atcagatcgc
      481 acaatccggg ctgagttccc tctaagtgat ctagg
//
LOCUS       DAJTNN010000411          510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTNN010000411 DAJTNN010000000
VERSION     DAJTNN010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.422"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..510)
                     /locus_tag="OO150_004348"
                     /pseudo
     CDS             complement(1..510)
                     /locus_tag="OO150_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          118 a          127 c          118 g          147 t
ORIGIN      
        1 cttccccttc gttctgacaa aggtaattcg gtatttggtc acctgtgagc gggtaagatt
       61 tacggcttca cgctagcgtg cgcctgtgct taagcatatc ttaactacca gtgccagaat
      121 tgggtcctga cgtttgcaat cagccagcaa ttcaacaatc tgctcatggg taagccatgc
      181 catctctttt tctgcgatgg tgaattttcg catgttctcc agtgggttcg gatacgacca
      241 ttcgcccaga cgggatagtt cgctaaaaac actacttaga tagctttgct ccaggttaat
      301 ggtgaccggg cttgctcctt tcttccattt ctcgctgaag tagatcccgc ctgtcaggcg
      361 tttatctcga tagtgggcaa acattttaga ggtgagatcg gttgcaagag gattgcccag
      421 agcgtcaacc atcaacagca atttgtcata gacatgctgc ccagctgtca gagatttacc
      481 atgtagtttg aaccatagct caaccacgtc
//
LOCUS       DAJTNN010000412          505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTNN010000412 DAJTNN010000000
VERSION     DAJTNN010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.423"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>505)
                     /locus_tag="OO150_004349"
     CDS             complement(<1..>505)
                     /locus_tag="OO150_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR5518244.1"
                     /translation="DGAGRHFQLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTE
                     YGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDD
                     KYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREV
                     LHTQGEAG"
BASE COUNT           81 a          161 c          154 g          109 t
ORIGIN      
        1 ccggcttcac cctgcgtgtg cagcacttca cggcggttca ggctgtcggt gatggtgatg
       61 cggtccttct catactgata cgtgtagctt aagcctgccg ggttaagctg ttctgtcacc
      121 cgcccgtcgc tgtcgtaacg gtaacggatt tccggccggc ccgtgtgacg gtgcgccacc
      181 atccggcccc ggtatttatc atcgtaagta aagctacgca cctgtgtatt gctgcggtca
      241 tacactgccg ccagttcgcc gcgcggcgtc cagccatagc gcaccagcgg cgcggcaggt
      301 aaattctccg ggtattccgg gtcgtgcgtc agccacacgg cagacagacg gatgccgttg
      361 tcccggccat attcggtgta acccggcagg gtatcaggaa aagcggacgg ttccgtcccg
      421 ccggaaatgg cctgctgccg ggcttcttct gcccgctgcg cctgcgtggt cagtaccagt
      481 tggaagtgac gcccggcacc atccg
//
LOCUS       DAJTNN010000413          497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTNN010000413 DAJTNN010000000
VERSION     DAJTNN010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.424"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>497
                     /locus_tag="OO150_004350"
     CDS             <1..>497
                     /locus_tag="OO150_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5518245.1"
                     /translation="GDYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALNA
                     MASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKM
                     LFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLV
                     SLPDF"
BASE COUNT          132 a          107 c          136 g          122 t
ORIGIN      
        1 tggtgactat gccattaatg ccgcgctgac agaaaaacga attaaagcag tagtgagtat
       61 tacgggtgtt aatatcgggc gtctttttcg ggaaggtttc agcaattacg atccgattgg
      121 cgcattgaat gcaatggcgt cgcaacgaac caaagaggct cgtggtggag aattacagat
      181 caacgaatta cttcccgcca gcctggatgc agcaaaagcc catggactga ccgaacgcga
      241 tgtttatgaa gcaactgact actataaaac accacgcggg caacaaccgg gaggtgcgac
      301 aaaaatgttg ttctcacatg cacagaaaac gctggcctgg gatgccttcg catttactga
      361 agtgttactg actcagccgg tgatggtagt cgttggtgaa aaagtcggtg ccttcggtgc
      421 ttatcgtgat ggactggaag tctatggccg ggcgatggtt tcgcaggatc gtcagttagt
      481 ttcattaccc gatttct
//
LOCUS       DAJTNN010000414          490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTNN010000414 DAJTNN010000000
VERSION     DAJTNN010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.425"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>490
                     /gene="ytfB"
                     /locus_tag="OO150_004351"
     CDS             <1..>490
                     /gene="ytfB"
                     /locus_tag="OO150_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCR5518246.1"
                     /translation="LVVGFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVT
                     PQNDPDQVAPVAPEPIQEGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLF
                     RDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFT
                     RQP"
BASE COUNT          131 a          137 c          135 g           87 t
ORIGIN      
        1 attggtggtc ggatttctgc ttccatctga tgatacgccc aacgcgccgg tggtgacgcg
       61 tgaagcgcag ttagatattc agtcgcaatc acaaccgccg acagaagaac agctgcgggc
      121 gcaattagtc acaccacaaa atgatcctga tcaggtggct ccggtcgctc cagaacctat
      181 acaggaaggc cagccagaag agcagccgca aactacccaa acgcaaccat tccagccaga
      241 tagcggaatt gataatcaat ggcgctctta ccgtgtcgaa cccggtaaaa cgatggcgca
      301 gctgttccgc gaccacggac tgcccgctac cgatgtctat gcaatggcgc aggtagaagg
      361 cgcgggtaag ccgctgagta atttgcaaaa cgggcagatg gtgaaaatcc gccagaacgc
      421 cagcggcgtg gtgacgggct taaccattga tacggggaat aatcaacagg tcctgttcac
      481 ccgccagccg
//
LOCUS       DAJTNN010000415          486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTNN010000415 DAJTNN010000000
VERSION     DAJTNN010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.426"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>486
                     /locus_tag="OO150_004352"
     CDS             <1..>486
                     /locus_tag="OO150_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5518247.1"
                     /translation="NAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIA
                     AAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDT
                     KSDFLAWVRNALPGEYSVAGPYDIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTF
                     YG"
BASE COUNT          126 a          129 c          137 g           94 t
ORIGIN      
        1 aatgcagccg cagccggaca gagcgcacag gatgccgcag gttatgcccg cgcagcagaa
       61 caggccaaaa atgacattga tgctgcgctg accggcactc tgaaaatggc taaccatctg
      121 tcagaaatcg cagcagcagg cgaaaaggca caacagaagt cccgggataa tctggggctg
      181 aaaagtgcgg ccacgatgga agcacagagc gatatttacg accggacaaa aggccgtctg
      241 gcgatacccg gcgcattcgg ctttgggtgt gcttttctgc ctgaagatgt tatccgtttt
      301 gacactaaga gtgatttcct ggcctgggta aggaatgcgc tgccaggtga atattccgtt
      361 gctggcccct acgacatcat catacccgac acacggtttg aaggggtgct cagcatccgg
      421 tggactgatg cacgccctga gacaacagaa ccgcggtaca gagccaaatc ccttactttt
      481 tacggc
//
LOCUS       DAJTNN010000416          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTNN010000416 DAJTNN010000000
VERSION     DAJTNN010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.427"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>480
                     /locus_tag="OO150_004353"
     CDS             <1..>480
                     /locus_tag="OO150_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5518248.1"
                     /translation="VEVNWIDPDNGHETATELVEDAQAILRYGRNVTKMDAFGCTSRG
                     QAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNS
                     QTRTLTLDREITLPSSCTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSV
                     "
BASE COUNT          103 a          124 c          160 g           93 t
ORIGIN      
        1 cgttgaggtg aactggattg atccggacaa cggtcatgag acggcgacag agcttgtgga
       61 ggacgcgcag gccattctcc gttacggtcg taacgtcacg aagatggatg cctttggctg
      121 taccagccgg ggacaggcgc accgcgccgg gctgtggctg attaaaacgg aactgctgga
      181 aacgcagacc gtggacttca gcgtcggcgc agaagggctt cgccatgtac cgggtgatgt
      241 cattgaaatc tgcgatgatg attatgccgg tatcagcacc ggtggtcgcg tactggcggt
      301 gaacagccag acccggacgc tgacgctcga ccgtgaaatc acgctgccgt cctcctgtac
      361 cacgctgata agcctggttg acggaaatgg caatccggtc agcgtggagg ttcagtccgt
      421 caccgacggc gtgaaggtga aagtgagccg ggttcctgac ggcgttgcag aatacagcgt
//
LOCUS       DAJTNN010000417          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTNN010000417 DAJTNN010000000
VERSION     DAJTNN010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.428"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..117
                     /locus_tag="OO150_004354"
     CDS             <1..117
                     /locus_tag="OO150_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5518249.1"
                     /translation="GLSRTQAKAFMAGGYGALSLRDAEDVSSALNALKNLNF"
     gene            132..>472
                     /locus_tag="OO150_004355"
     CDS             132..>472
                     /locus_tag="OO150_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR5518250.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGRGDGLRDL
                     ERKALQVGTDE"
BASE COUNT          137 a          106 c          147 g           82 t
ORIGIN      
        1 ggactctccc gtacccaggc caaagcattc atggccgggg gctatggcgc actgtccctg
       61 cgcgacgctg aggatgtgag ctctgcactg aatgcactga aaaatctgaa cttctaatca
      121 ggagaaatac gatggcggta gatattaaag atgtcgaaca ggtcgcgcag gagctgcagc
      181 agaagtttga cgacttcaaa gcaaagaacg acaagcgcgt ggatgcgatt gagcaggaaa
      241 aaggcaagct tgccgggcag gtggaaaccc tgaacgggaa actcagcgag ctggaaaacc
      301 tcaaaagcga tcttgaaaaa gagctgcttg agctgaaacg tccggcaggt ggtgcgcaaa
      361 ataaactggc caccgagcat aaagaagcgt ttgtgggctt cctgcgtaaa ggccgtggag
      421 atggtctgcg cgatctggag cgcaaggcat tacaggtggg caccgatgaa ga
//
LOCUS       DAJTNN010000418          466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTNN010000418 DAJTNN010000000
VERSION     DAJTNN010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.429"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            107..466
                     /locus_tag="OO150_004356"
                     /pseudo
     CDS             107..466
                     /locus_tag="OO150_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          121 a          115 c          110 g          120 t
ORIGIN      
        1 tttaacgtaa ggtgagagga tttttaagtg tatccataga ttaaaaatga caatgcctcc
       61 aattagttga attaggggtt ataatacccg cttttaaggt tctttcatgg tcacagttaa
      121 ctttcattgc ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaagg
      181 acatgatcga ttccgttgcc gtgactgtca ccgggtgttt cagctcactt actgttacga
      241 ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg gcttcaacgg tgccggagtt
      301 cgcgatactg cccgtacgct gaaaatcggc atcaataccg tcatacggac attaaaaagc
      361 tcccgccccg gcggataaca tctgcgccag tcgctcacgc tgatgttgcc cttatctgtg
      421 aacttgatgg gcagtggagc ttcgttggca gcaaggcgca acaaca
//
LOCUS       DAJTNN010000419          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTNN010000419 DAJTNN010000000
VERSION     DAJTNN010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.430"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>450
                     /gene="curA"
                     /locus_tag="OO150_004357"
     CDS             <1..>450
                     /gene="curA"
                     /locus_tag="OO150_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5518251.1"
                     /translation="GPVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHH
                     AGDFAEQLVKACPKGIDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPG
                     PDRLPLLMATVLKKRIRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKI"
BASE COUNT          110 a           92 c          137 g          111 t
ORIGIN      
        1 caggtcctgt gggagcaacg gtggggcaaa tcggcaaact taaaggttgc agggtggtgg
       61 ggggggccgg tggcgcggaa aaatgccgcc atgctatcga ggtgctgggc ttcgatgttt
      121 gtcttgatca ccacgcgggt gattttgccg aacaactggt gaaagcgtgc ccaaaaggta
      181 ttgatatcta ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta
      241 atacatctgc gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac
      301 cacccggtcc ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct
      361 tgcaagggtt tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga
      421 tggggcaatg ggtgaaagag gataaaatcc
//
LOCUS       DAJTNN010000420          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTNN010000420 DAJTNN010000000
VERSION     DAJTNN010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.431"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..405
                     /gene="nudI"
                     /locus_tag="OO150_004358"
     CDS             <1..405
                     /gene="nudI"
                     /locus_tag="OO150_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5518252.1"
                     /translation="CPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEYGERIEEALR
                     REIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKINE
                     EFQDYTWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          125 a           90 c          119 g          116 t
ORIGIN      
        1 tgccctttaa ttcaaaatga tggtgcttat ctgctgtgca aaatggccga cgatcgcggc
       61 gttttccccg gtcaatgggc gctttcgggt ggcggcgtgg agtatggcga acgaattgaa
      121 gaggcactac gccgcgaaat tcgcgaagaa ctgggagaac agctgctttt gacagaaatc
      181 acgccgtgga ccttcagcga tgatattcgc accaagacgt atgcagatgg tcgcaaggaa
      241 gagatttata tgatttacct gatttttgac tgcgtttctg ccaaccgaga agtgaaaata
      301 aacgaagagt ttcaggacta cacgtgggta aaacctgaag atctggtgca ttatgatttg
      361 aatgttgcca cccgaaaaac gttacgtttg aaaggtcttc tttaacaatg atgacagcca
      421 tcactggctg tcagattgaa aatttagtgg
//
LOCUS       DAJTNN010000421          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTNN010000421 DAJTNN010000000
VERSION     DAJTNN010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.432"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            210..>450
                     /locus_tag="OO150_004359"
     CDS             210..>450
                     /locus_tag="OO150_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR5518253.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGT"
BASE COUNT          113 a          117 c          113 g          107 t
ORIGIN      
        1 ttcacaaggt ctggaagcga tgattcgcgc aattcgcgcc aaagccgctg cacttagcta
       61 aactaaaggg acagctttca tccggttggc tcacattgcc ggatggtcaa tgtttacact
      121 gacctgcaca ggagtgtttt ggtttcaggt gaacataagc gccatccgac gtttcagcgt
      181 gagtctccgg caaatacagg aggtttacaa tgaaacgcgc gtctttgctt acactcacgc
      241 ttatcggcgc ttttagcgcc atccaggctg cctgggcggt tgattatccg ctaccaccaa
      301 ccggaagccg actggttggg caaaatcaaa cgtatacggt gcaagaaggg gataaaaacc
      361 ttcaggctat tgcccgacgt tttgatactg cggcaatgtt gatccttgaa gccaataaca
      421 ctatcgcccc ggtgccaaaa cctggtacga
//
LOCUS       DAJTNN010000422          448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTNN010000422 DAJTNN010000000
VERSION     DAJTNN010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.433"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..332)
                     /locus_tag="OO150_004360"
     CDS             complement(<1..332)
                     /locus_tag="OO150_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5518254.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPE"
BASE COUNT          140 a           95 c          108 g          105 t
ORIGIN      
        1 ctttccggta aaaataaaaa cggtaaatag agcgcaatat cggaaataac atcgccagtt
       61 tcattcaaaa tcgcccccag acgtgtttgc tgattgcact cacgcgccaa catgccatcc
      121 agcgcattga gcgccatacg gataaaaagc acgatgggca atagcaaaaa gaggatgggt
      181 tgtgccacca acaccaacag caatccggta aaaagagaaa gcgccagtgc cgtaagagtg
      241 atgtgattcg ctgtaacgtg gtgcttataa agccaaaaca tcgtcggcct taacagcgac
      301 tgaaagagcg gttttatctg gtagagcgtc atttatcatt ccgtgatatt cagtttgctc
      361 tatggtgcat caattgtgat ggcggagata tcacaacgcg gagcatcaac gtaaattcgg
      421 aatatatcaa gatgtggtgg atggtttt
//
LOCUS       DAJTNN010000423          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTNN010000423 DAJTNN010000000
VERSION     DAJTNN010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.434"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            35..447
                     /locus_tag="OO150_004361"
                     /pseudo
     CDS             35..447
                     /locus_tag="OO150_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          101 a          115 c          121 g          110 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacagtggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcac
//
LOCUS       DAJTNN010000424          446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTNN010000424 DAJTNN010000000
VERSION     DAJTNN010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.435"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>446
                     /locus_tag="OO150_004362"
     CDS             <1..>446
                     /locus_tag="OO150_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5518255.1"
                     /translation="GGGNYPDCGAVMDDATYQKTFDEENQVEVQENDPEEMEGAEHPH
                     KENAGSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHLMIDLETMGKNPDAPII
                     SIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREA"
BASE COUNT          140 a          102 c          118 g           86 t
ORIGIN      
        1 ggtggcggca actaccctga ttgtggcgcg gtgatggacg acgcaacata ccagaaaaca
       61 ttcgatgaag agaatcaggt tgaagttcag gaaaatgatc cggaggaaat ggaaggcgct
      121 gaacatccac acaaggagaa tgctggcagc gctcaggacc acgccagcga tagtaaaact
      181 ggcgagacgg cagatccctt aattacggtg aatggtcatc acgaaatcac atccaccagc
      241 aggacgtgtg accatctaat gatcgacctt gaaaccatgg gaaaaaatcc tgatgccccg
      301 atcatctcaa taggtgcaat atttttcgat ccgcaaaccg gagatatggg acccgaattt
      361 agtaagacta tcgatctgga aactgctggc ggagtcattg atcgggacac cattaaatgg
      421 tggcttaagc aatcacgcga agcgca
//
LOCUS       DAJTNN010000425          445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTNN010000425 DAJTNN010000000
VERSION     DAJTNN010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.436"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>445
                     /gene="pocR"
                     /locus_tag="OO150_004363"
     CDS             <1..>445
                     /gene="pocR"
                     /locus_tag="OO150_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HCR5518256.1"
                     /translation="CHAGLTDFSIPLVIAGHLVGFVLCGQVRLRNDYVDLVDILNVDD
                     CWQADPELLNEFRKVPEMDYSRVMASADLLKLIVENCLKKQLNFVVIKENKSQSDSVR
                     QTRAPSPHDNKMKKALRYIDAHLSDDLRLEDVASHVYLSPYYFSKL"
BASE COUNT          117 a           86 c           98 g          144 t
ORIGIN      
        1 tgtcatgccg gattaaccga tttctctatt ccactggtaa tcgccgggca tttagtcggt
       61 tttgtattgt gcgggcaagt tcgtctgcgt aatgattatg tcgatttggt cgatattctg
      121 aatgtcgatg attgctggca ggcagatccc gaattactta atgaatttcg caaagttcct
      181 gagatggatt actcaagagt tatggcctct gctgatttgt tgaagttgat tgtagaaaat
      241 tgcctcaaaa aacaactcaa ttttgtggtt attaaagaga acaaatctca gtcagattcg
      301 gtgcgccaga ctcgtgcgcc tagtccccac gacaataaaa tgaaaaaagc tttacgctat
      361 attgatgcgc atttatcaga cgatttgcgt ctggaagatg tcgcctcgca cgtttatcta
      421 agcccttatt atttcagtaa gttat
//
LOCUS       DAJTNN010000426          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTNN010000426 DAJTNN010000000
VERSION     DAJTNN010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.437"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(165..>439)
                     /locus_tag="OO150_004364"
     CDS             complement(165..>439)
                     /locus_tag="OO150_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5518257.1"
                     /translation="RIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMP
                     ELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
BASE COUNT           90 a          147 c          104 g           98 t
ORIGIN      
        1 cacagaccgg actgactccg gaaaaacaga ggcccgcccc cgggcctccc cggattcatc
       61 cgtttccctg ttcagcctga cagcacgccc ccgcggccgg atgacagact ccgcttcggt
      121 aagcaaagcg gtcttctgtg attccgccag ttgcggctta ttcattactc aacgtcaaac
      181 gcccgaattg aagccaaatc atccagaccg ctcagctcct ctttcatttc acgctgacgg
      241 cgataaatct catcgttgcg atcgacctgc gcctgcacca ttgctgccgc cagttcttcc
      301 agttccggca tcgacagttt cacctgctga ttatcggcat cgctccacgc catatgtgtt
      361 tgtgctgtga cagattttgc cagcatgact accggggaca ggcggcccag tgagtcggga
      421 ccagcattcc agatacgac
//
LOCUS       DAJTNN010000427          437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTNN010000427 DAJTNN010000000
VERSION     DAJTNN010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.438"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..396)
                     /locus_tag="OO150_004365"
     CDS             complement(<1..396)
                     /locus_tag="OO150_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HCR5518258.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETL"
BASE COUNT           99 a          111 c          110 g          117 t
ORIGIN      
        1 cagcgtttct tcgaggaata ccgccacttc gatttcaata tcgtggccgc gtgcggcggg
       61 gcgttgacgg ctctggcggg catgctgacc gaaaattgac gagaagatat cgtcaaaatc
      121 ttcggcatta aaactctgac cgtcgccatg atggaactga cggttaaatt gcggatcgtt
      181 gcgatgttgc cacatctgat catactcagc gcgacgttgt tcatcactta acacttccca
      241 ggcttcagcg acctctttga agcgggcttc ggcatccggt tctttgctga catcaggatg
      301 gtatttacgg gccagtcgac gataggcggt cttgattgtc ttgagatcgt ccgtcggttt
      361 cacgcccatg atggcgtaat aatcctttaa ttccatagcg ttatctcgcg taaatcaaca
      421 caaattgaag gaacccc
//
LOCUS       DAJTNN010000428          437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTNN010000428 DAJTNN010000000
VERSION     DAJTNN010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.439"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>437)
                     /locus_tag="OO150_004366"
     CDS             complement(<1..>437)
                     /locus_tag="OO150_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCR5518259.1"
                     /translation="IPLWDDATLSLMAKYSAPNKTDQQQDNENDNSYFEMKDSWMLTS
                     VLRQNLQRDTFNEFTLQVANNSYASSFASFSDASNTMAHGRYYYGDHTNGIAWRLISQ
                     GEMYLTDNIIMANALVYSHGEDVYSYESGAHSDFDSIRTVIRP"
BASE COUNT          132 a           92 c           83 g          130 t
ORIGIN      
        1 cggtcttatt acagtgcgaa tactgtcaaa atcactatga gcaccacttt cataactata
       61 aacatcttcg ccgtgagaat agacaagcgc gttagccata ataatattgt cagtaagata
      121 catctcgccc tgagagatta aacgccaggc gatcccattg gtatgatcac catagtaata
      181 gcgaccatgc gccatcgtat tactggcatc tgagaaactg gcaaaactgc tggcatagga
      241 attattggca acctgtaacg taaattcatt aaacgtatcg cgttgcaggt tttgccgtaa
      301 aacagaagtc agcatccagc tatctttcat ttcaaaataa ctgttgtcat tttcgttatc
      361 ttgttgttga tccgttttat taggtgcgga atatttagcc attaatgata atgtcgcatc
      421 atcccataac gggatat
//
LOCUS       DAJTNN010000429          436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTNN010000429 DAJTNN010000000
VERSION     DAJTNN010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.440"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>436)
                     /gene="adeP"
                     /locus_tag="OO150_004367"
     CDS             complement(<1..>436)
                     /gene="adeP"
                     /locus_tag="OO150_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5518260.1"
                     /translation="FTTFLTMVYIVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMG
                     LFANLPVALAPAMGLNAFFAFVVVQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMI
                     ANIPVSLRVGITSGIGLFIGMMGLKNAGVIVANSETLVSIGNL"
BASE COUNT          137 a          125 c          100 g           74 t
ORIGIN      
        1 agattaccga tgctcaccag cgtttccgag ttagcgacaa tcacacctgc gtttttcagc
       61 cccatcatgc cgataaacag gccgataccg ctggtaatac ccacacgcag acttaccgga
      121 atgttagcaa tcatccagta acgaacgcgg aaaatggtca aaagaagcag tccgacagcg
      181 ccccagaaga ttgcgcccat accgatctgc cacggcagcc ccattgcctg gacaaccaca
      241 aaagcgaaaa aggcgttaag ccccatcgca ggagccagcg ccaccggaag gtttgcaaac
      301 aatcccatca taatgctacc gaaagcagca atcagacagg ttgttacgaa gacggcactg
      361 gtgtccatgc cagcaacgcc cagaatttgt gggttaacaa aaacgatgta aaccatcgtc
      421 aggaaggtgg taaaac
//
LOCUS       DAJTNN010000430          432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTNN010000430 DAJTNN010000000
VERSION     DAJTNN010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.441"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>432
                     /locus_tag="OO150_004368"
     CDS             <1..>432
                     /locus_tag="OO150_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5518261.1"
                     /translation="NKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAY
                     KNFFQNRAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVK
                     NVTVSQSCGKWYISIQTESEVSTPVHPSASMVGLDAGVAKL"
BASE COUNT          117 a          105 c          112 g           98 t
ORIGIN      
        1 gtaataaata catcccttac gggaaaatgg cttcctggct ggttgagtgg aaaaatgcca
       61 ctgaaacgca atggcttaaa gatgccccgt cacagccatt gcaacagtca ctgaaagacc
      121 ttgagcgggc ttacaaaaac ttcttccaga atcgggctgc ttttccccga ttcaaaaagc
      181 ggggacagaa tgatgtattc cgctacccgc agggcgttaa gctcgatcag gaaaacagcc
      241 gtatttttct gccgaaactg ggctggatgc gctaccggaa cagccgtcag gtcacgggtg
      301 ttgtgaaaaa tgtcactgtc agccagtcct gcggtaagtg gtacatcagt attcagacag
      361 aaagtgaagt atcaactccg gttcaccctt cagcatcaat ggtcgggctg gatgctggcg
      421 tggctaaact cg
//
LOCUS       DAJTNN010000431          426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJTNN010000431 DAJTNN010000000
VERSION     DAJTNN010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.442"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>426
                     /locus_tag="OO150_004369"
     CDS             <1..>426
                     /locus_tag="OO150_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5518262.1"
                     /translation="HHPDKRSARAQRDDWLKKEILRVYDGNHQVYGVRKVWRQLLREG
                     IRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADF
                     TYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDA"
BASE COUNT           91 a           98 c          136 g          101 t
ORIGIN      
        1 acatcatcct gataaacgca gtgcccgtgc gcagcgcgat gactggctga agaaagagat
       61 actgcgcgta tacgatggga atcatcaggt atacggtgtg cgtaaagtct ggcgtcagtt
      121 gttacgggaa ggtatcagag tggccagatg cactgtggca cgtctcatgg cggttatggg
      181 acttgccggt gttctccggg gtaaaaaggt ccgtacgacc atcagccgga aagccgttgc
      241 cgcaggcgac cgcgtaaacc gtcagttcgt ggcagaacga cctgaccagc tgtgggtggc
      301 tgattttact tacgtcagca catggcaggg cttcgtctat gtggcgttca ttattgatgt
      361 gtttgccgga tacatcgtgg ggtggcgggt ctcatcgtcc atggaaacga cattcgtgct
      421 ggatgc
//
LOCUS       DAJTNN010000432          426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTNN010000432 DAJTNN010000000
VERSION     DAJTNN010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.443"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>426)
                     /locus_tag="OO150_004370"
     CDS             complement(<1..>426)
                     /locus_tag="OO150_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR5518263.1"
                     /translation="VGGIYQIAAEFERYALRMASCSGLLRFGWSTIMETGETRLRLRS
                     AQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVDYPSSRWLFLTLTVRNCEIGELGTV
                     LTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDGSAHP"
BASE COUNT          108 a          124 c          101 g           93 t
ORIGIN      
        1 gcggatgtgc gctgccatcc ttacctcgcg tcacctccgt agccctgatc cacccctgaa
       61 caggtgatag ctcctttcgc ttttccattc gcttaaacgc cgcattcatt gctgtaagga
      121 ctgttccaag ttcacctatc tcgcagttcc tgacggttaa cgtcagaaac aaccatcggg
      181 aagacgggta atccacaacg attttcggta gagcctgata aaaacgggct tgccacatga
      241 gggttcttct ccactggcag acagggcaat gacggacacg gcaaaattgc gcactacgaa
      301 gccgtaggcg cgtttctccg gtttccatga tggtagacca accaaaacgt aacaaaccgc
      361 tacaggaggc catacggagt gcatagcgct cgaattcagc agcaatctgg tagatacccc
      421 cgacag
//
LOCUS       DAJTNN010000433          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJTNN010000433 DAJTNN010000000
VERSION     DAJTNN010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.444"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..380)
                     /locus_tag="OO150_004371"
     CDS             complement(<1..380)
                     /locus_tag="OO150_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5518264.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATP"
BASE COUNT          108 a          107 c          127 g           82 t
ORIGIN      
        1 tgcggcgtgg cgttatgcca gagctgcgct gttttcgtga acacgcggcg gacagcctca
       61 cttcccaggc gctggcgggc ctgaataacg gcactggggg caacgaaggg gcgattgccc
      121 ggcagcatga tgtccaggcg attcacaatc tggtgaagag gttctttacg ctcaagcgcc
      181 atgccaacaa tacaccagac catcatttcg aggggaagac ggcgcttgcg tagcgttaca
      241 gtacctgatt cggcaaggca acgagagatg agttcggggt cgaggtaatc ccccagagaa
      301 gtcagtgggt tacgcagaga atcgtaacgg gataccagat caagagcctg tccaatgtgc
      361 ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc
      421 aact
//
LOCUS       DAJTNN010000434          420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJTNN010000434 DAJTNN010000000
VERSION     DAJTNN010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.445"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            26..>420
                     /locus_tag="OO150_004372"
     CDS             26..>420
                     /locus_tag="OO150_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCR5518265.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPA"
BASE COUNT          101 a          114 c          109 g           96 t
ORIGIN      
        1 ccgtttctta cctgattatt cccccatgaa tcgtgattcc ttttatccag ccatcgcctg
       61 ttttccgctg ttattgatgc tggccgggtg tgcgcctatg catgaaaccc gccaggcgtt
      121 aagccagcaa acgcccgctg cacaagttga caccgcatta cccacggcgc tgaaaaatgg
      181 ttggccagac agccaatggt ggctggagta tcacgataat cagctcactt cattaattaa
      241 caatgcacta cagaacgcac cggatatgca ggtggctgaa cagcgcattc agctagctga
      301 agcccaggcg aaggcagttg ccactcagga tggtccgcag atcgactttt cggcggatat
      361 ggagcggcaa aaaatgtcgg cagaaggctt aatggggccg tttgctctga acgatccggc
//
LOCUS       DAJTNN010000435          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJTNN010000435 DAJTNN010000000
VERSION     DAJTNN010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.446"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>410
                     /locus_tag="OO150_004373"
     CDS             <1..>410
                     /locus_tag="OO150_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5518266.1"
                     /translation="RAHYPVVTLCQVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLEL
                     HGISHGSAGARSIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAI
                     PNYLERQFAVTEPNQVWCGDVTCIWTGKRWAYLA"
BASE COUNT           97 a          106 c          125 g           82 t
ORIGIN      
        1 cagagcgcat tatcctgtgg tcacactctg ccaagtgttc ggggttcatc gcagcagcta
       61 cagatactgg aaaaaccgtc ctgaaaaacc agacggcaga cgggctgtat tacgcagtca
      121 ggtacttgag ctacatggca tcagccatgg ttcggccgga gcaagaagca tcgccacaat
      181 ggcaacccgg agaggctacc agatgggacg ctggcttgct ggcaggctca tgaaagagct
      241 ggggctggtc agctgtcagc agccgactca ccggtataaa cgtggtggtc atgaacatgt
      301 tgctatccct aactaccttg aaaggcagtt cgccgtgacc gagccaaatc aggtgtggtg
      361 cggtgatgtg acctgtatct ggacgggtaa gcgctgggcg tacctcgccg
//
LOCUS       DAJTNN010000436          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJTNN010000436 DAJTNN010000000
VERSION     DAJTNN010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.447"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..309)
                     /locus_tag="OO150_004374"
     rRNA            complement(<1..309)
                     /locus_tag="OO150_004374"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a          114 c           84 g          125 t
ORIGIN      
        1 tccagacgct tccactaaca cacacactga ttcaggctct gggctgctcc ccgttcgctc
       61 gccgctactg ggggaatctc ggttgatttc ttttcctcgg ggtacttaga tgtttcagtt
      121 cccccggttc gcctcattaa cctatggatt cagttaatga tagtgtgacg aatcacactg
      181 ggtttcccca ttcggaaatc gccggttata atggttcata tcaccttacc gacgcttatc
      241 gcagattagc acgtccttca tcgcctctga ctgccagggc atccaccgtg tacgcttagt
      301 cgcttaacct cacaacccga agatgtttct tacgattcat catcgtgttg cgaaaatttg
      361 agagactcac gaacaacttt cgttgttcag tgtttcaatt ttcagcttga
//
LOCUS       DAJTNN010000437          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJTNN010000437 DAJTNN010000000
VERSION     DAJTNN010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.448"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            108..>410
                     /gene="hlyE"
                     /locus_tag="OO150_004375"
     CDS             108..>410
                     /gene="hlyE"
                     /locus_tag="OO150_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR5518267.1"
                     /translation="MTEIVADKMVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLF"
BASE COUNT          138 a           68 c           84 g          120 t
ORIGIN      
        1 ttgacgcatc ccacccggct aactatgaat tagatgaagt aaaatttatt aatagttgta
       61 aaacaggagt ttcattacaa tttatatatt taaagaggcg aatgattatg actgaaatcg
      121 ttgcagataa aatggtagaa gtagttaaaa acgcaatcga aaccgcagat ggagcattag
      181 atctttataa taaatatctc gatcaggtca tcccctggca gacctttgat gaaaccataa
      241 aagagttaag tcgctttaaa caggagtatt cacaggcagc ctccgtttta gtcggcgata
      301 ttaaaacctt acttatggat agccaggata agtattttga agcaacccaa acagtgtatg
      361 aatggtgtgg tgttgcgacg caattgctcg cagcgtatat tttgctattt
//
LOCUS       DAJTNN010000438          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJTNN010000438 DAJTNN010000000
VERSION     DAJTNN010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.449"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            5..>408
                     /locus_tag="OO150_004376"
     CDS             5..>408
                     /locus_tag="OO150_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518268.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVII"
BASE COUNT          163 a           65 c           78 g          102 t
ORIGIN      
        1 aaaaatggga aaaatgaata ttaactttaa acatatcctt cctttattgt cgtcattttt
       61 tatttcagga tgcgactctg atggaataag caaggtaaag tctttcgtat attacgacat
      121 tgacgactca atgactgttg gtaaagcctt cgatacaagg agtgattgca tcaatggcac
      181 atggagtgaa aaagaggatg atcgaggaag aaccatcgtc acatatacct gtgatattag
      241 tgaaagtggc ctaaaaataa taaattcagc cattatgcaa gaacctgaag ataaggctga
      301 atattctata gcacaaaata acaacagcat aaaatcaacc aatgaatcac tagaggaata
      361 caaacagaaa acgcctctgg tggaaaaaac aaaagaggtc ataataga
//
LOCUS       DAJTNN010000439          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJTNN010000439 DAJTNN010000000
VERSION     DAJTNN010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.450"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>397
                     /locus_tag="OO150_004377"
     CDS             <1..>397
                     /locus_tag="OO150_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5518269.1"
                     /translation="ENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNL
                     LNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKA
                     LFMALRSQRPPAGLIHHSDRGSQYCAYDYR"
BASE COUNT          102 a          107 c          109 g           79 t
ORIGIN      
        1 agagaatggc atcatcgttg gtcgtgaccg actggcacgt cttcgtaagg agctaaggct
       61 acgctgtaag cagaaacgca agttcagagc gactacgaac ccgaaccaca atctgccagt
      121 tgcgccaaat ctgctgaacc agacgttcgc tcctacagca ccaaatcagg tctgggtggc
      181 ggacctgacg tatgttgcca cacaggaggg atggttgtac ctcgctggca tcaaagatgt
      241 ttatacgtgc gaaattgtcg gctacgccat gggagagcgc atgacaaaag agctgacagg
      301 taaagccctg tttatggcgc tcaggagcca gcgcccacct gccgggctaa tccaccactc
      361 tgatcgaggt tcacagtact gcgcatacga ttaccgg
//
LOCUS       DAJTNN010000440          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJTNN010000440 DAJTNN010000000
VERSION     DAJTNN010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.452"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>394
                     /locus_tag="OO150_004378"
     CDS             <1..>394
                     /locus_tag="OO150_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005042364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late control protein"
                     /protein_id="HCR5518270.1"
                     /translation="QFRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSS
                     SREGDYMEGAEGNVFVLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYP
                     EMHGTVTGFKSEIDYQDWTIAKAEHTIDN"
BASE COUNT          121 a           95 c          112 g           66 t
ORIGIN      
        1 cagttccgca ttgctgaccg cgaggcgtat acgggggtac gcgcctactg gcttgatctt
       61 aattacggga aaaagaaaaa agtcagcgtg aaacgccgca aaccgccaaa acccaaaaag
      121 gagaaaagca gcagtcggga aggcgactat atggaaggtg cggaaggcaa cgtgtttgtg
      181 ttacgcaaga cttatcagaa cgagcaggca gcaagacgcg cagcggcggc aaagtggcag
      241 cagctacaac gcggagccgc atcattctcc atcacgctgg cgcgtggtcg tgcagaactc
      301 tatcccgaaa tgcatggcac agtgacaggc tttaaaagcg agattgatta tcaggactgg
      361 actattgcaa aagccgagca caccattgat aaca
//
LOCUS       DAJTNN010000441          392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJTNN010000441 DAJTNN010000000
VERSION     DAJTNN010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.453"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..260)
                     /locus_tag="OO150_004379"
     CDS             complement(<1..260)
                     /locus_tag="OO150_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="catalyzes the conversion of 5-methoxyuridine to
                     uridine-5-oxyacetic acid in tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5518271.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIA"
BASE COUNT          122 a           99 c          103 g           68 t
ORIGIN      
        1 gcaataattt tgcaattatc atgatgaatg ttgcgacgca ccgagagcgt cgccgcgccc
       61 agagaacaac ccagatcgta aacctgcgta ccaggttgaa cgaagcgctc ggctaacata
      121 ctaatcatgg aaataatatt ggaatagccg ggaacggaac gctggatcat atccgggaag
      181 acttcagcta cccgttcatc aaaggtccag tcgcccagtc tggcgatagg ggcagaaaat
      241 agcgtgtcgc ggtgagacat aacgtaaaaa tccgggaaaa agaaagtggc gtattgtgcg
      301 ctaacgcagg gagaaaacca actcccaggg catataccaa agattcgcca gcaccatcag
      361 caacagcgca caccaggtgg cgctcatgcc gg
//
LOCUS       DAJTNN010000442          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJTNN010000442 DAJTNN010000000
VERSION     DAJTNN010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.454"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            187..342
                     /locus_tag="OO150_004380"
                     /pseudo
     CDS             187..342
                     /locus_tag="OO150_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          122 a           84 c           97 g           82 t
ORIGIN      
        1 acagagagca gaacgggatc tatacttttg cgggtcaaca taagaggatg agtccttaat
       61 aaaacaggtc ctcagagaat acatattctg cggagcgtgc ggcaacaaca ggacgaacag
      121 gggcggtttt caatttccag ccgtcaggct gccgtggttc actccaacaa cggagacagg
      181 taaattatgg acgaaaagaa gcttaaagcc cttgcggctg aactggctaa aggcctcaaa
      241 accgaggccg acctcaatca gttttcccgt atgcagacga aattaactgt cgaaacggtg
      301 cttaatgagg aactaactga ccatctcggg catgaaaaaa gctacatccg gtagtaaaca
      361 ttatcagtgg gttatgcgat atgat
//
LOCUS       DAJTNN010000443          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJTNN010000443 DAJTNN010000000
VERSION     DAJTNN010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.455"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            14..313
                     /locus_tag="OO150_004381"
     CDS             14..313
                     /locus_tag="OO150_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5518272.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMEFGERTVIKNPEYFSTYMQEELKALV"
BASE COUNT          131 a           68 c           90 g           96 t
ORIGIN      
        1 taaggataag aatatgcctg ctgtaataga taaagccctg gatttcattg gtgccatgga
       61 tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcaca gcgaagggaa tctttaaata
      121 tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt acggcgcgag ctgaccagga
      181 aggctggaac cctgggttca cggaaaaaat ggttggatgg gcaaaaaaaa tggagtttgg
      241 tgaacgcact gtaattaaaa atcctgaata cttttcaaca tatatgcagg aagaactaaa
      301 agcactggtc tgagtttaat ttatatcagc ataaatgggt caggacgctt ttaatcacat
      361 ataaaaagcg tcctttttct cacaa
//
LOCUS       DAJTNN010000444          383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJTNN010000444 DAJTNN010000000
VERSION     DAJTNN010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.457"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>383
                     /locus_tag="OO150_004382"
     CDS             <1..>383
                     /locus_tag="OO150_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5518273.1"
                     /translation="LSANQIEGDLVKTVGKPFPRDSRAPERWPSGTITVRVYDDQPFD
                     RQIVIPAVAFRGAKHERKNNNIYSSCSLIVKKNGAEIYNRTTLDNTLIYTGVIDMPAG
                     HGHMTLEFSVSAWLVNGWYPTASIS"
BASE COUNT           99 a           86 c          104 g           94 t
ORIGIN      
        1 ttgtccgcga accagattga aggcgatctc gttaaaacag tgggcaaacc tttcccacgg
       61 gactcccggg caccggagag gtggccatca gggaccatta ccgtcagggt ttatgacgat
      121 cagccgtttg atcggcaaat tgttattccc gcggtggcgt ttcgcggtgc taaacatgag
      181 cggaagaata acaatattta ttcgtcatgc agcctgatag tgaagaaaaa cggtgctgaa
      241 atttataacc gaacgaccct ggataatacg ctgatatata cgggtgttat tgatatgcct
      301 gccggtcacg gtcacatgac gctggagttt tctgtatcgg catggctggt aaatggctgg
      361 tatcccacag caagtatcag cga
//
LOCUS       DAJTNN010000445          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJTNN010000445 DAJTNN010000000
VERSION     DAJTNN010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.458"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            257..>375
                     /locus_tag="OO150_004383"
     CDS             257..>375
                     /locus_tag="OO150_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518274.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEF"
BASE COUNT          118 a           88 c           70 g           99 t
ORIGIN      
        1 ggcatcagcc acttcttgtg ggcccgaata tggtttacca taaatactac gcagttttgt
       61 gtcgcttata gccataaata atatttggta cgctcagaaa taatggtttg gtacacatct
      121 ggtacacaat atcacatgga caacagcgca cagtaaacaa ctatatttga cgttcgtaga
      181 catactcagg tgaaaaaatg gttgtttttc gagttataac agacagttaa ttaactacag
      241 tggacaatgc tagacaatga cagacacaga taaacaacct accttcctct ttcacgatta
      301 cgaaaccttt ggcacgcacc ccgcgttaga tcgccctgca cagttcgcag ccattcgcac
      361 cgatagcgaa ttcaa
//
LOCUS       DAJTNN010000446          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJTNN010000446 DAJTNN010000000
VERSION     DAJTNN010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.459"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>372)
                     /locus_tag="OO150_004384"
     CDS             complement(<1..>372)
                     /locus_tag="OO150_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR5518275.1"
                     /translation="RREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELGRA
                     REPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREGM
                     PESHETMARPERVRMYSGATVR"
BASE COUNT           65 a          136 c           84 g           87 t
ORIGIN      
        1 cggaccgtcg caccggaata cattctcaca cgctcaggcc gcgccatcgt ctcatgagat
       61 tctggcattc cctcgcgtat atccgagacc atcacagccc cgcctggcga caggtaaaca
      121 ttcaccgggt catgaacact ctgggggtaa tgcgccagct catcacccca taccgtgaat
      181 tctccggctc tgttactcaa catcatgtag cgggttatcc tgcccgttcc cggctctctg
      241 gcacgcccca gctccgtaat aaccccgact aatgccgttt cacggctcag acggtggtta
      301 tcatgacgtt tcagccgcgc ctcatgctcc tgttccttcc ggcgcgcttc cgcttcggct
      361 tcctccctgc gc
//
LOCUS       DAJTNN010000447          367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJTNN010000447 DAJTNN010000000
VERSION     DAJTNN010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.460"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>367
                     /gene="ldtE"
                     /locus_tag="OO150_004385"
     CDS             <1..>367
                     /gene="ldtE"
                     /locus_tag="OO150_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5518276.1"
                     /translation="VNLAELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIP
                     NPTWTPTAGIRQRSLERGIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDSV
                     GLRVSSGCIRMNAPDIKALF"
BASE COUNT           83 a           94 c          101 g           89 t
ORIGIN      
        1 cgttaacctt gcagagctgc gcctttatta ttatccgccg ggagaaaata tcgtgcaggt
       61 ttatccgata ggtattggct tgcaggggct ggaaacgccg gtgatggaaa cgcgtgtggg
      121 gcagaaaatc cctaatccaa cctggacgcc tacggcaggc attcgtcaac gttcgctgga
      181 gcgtggcatt aaattactgc cagtcgttcc tgccggacca aataacccgc taggacgtta
      241 cgcactgcgc ttcgcgcatg gtaatggcga atacctcatt catggtacca gtgcgccgga
      301 cagcgtcggt ttacgcgtca gttcgggctg tattcgcatg aatgcaccgg atattaaagc
      361 cttattc
//
LOCUS       DAJTNN010000448          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJTNN010000448 DAJTNN010000000
VERSION     DAJTNN010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.461"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..300
                     /locus_tag="OO150_004386"
     CDS             <1..300
                     /locus_tag="OO150_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5518277.1"
                     /translation="KTGRTFADEHYLKSRVTVVDVSAPDVLALLKELEVKDKRIAELT
                     DALTQMINAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
BASE COUNT          122 a           69 c           83 g           90 t
ORIGIN      
        1 aaaactggtc gaacttttgc ggatgagcat tacctgaaat cgcgcgtaac cgtcgttgat
       61 gttagcgctc ctgacgtact ggctttactg aaagagctgg aagttaaaga caaacgcatt
      121 gcagaattga cagatgcgct tacacaaatg attaatgcgc acaaaaccac aattcgtttt
      181 ggtcatgaac gcataactga atgtggtggt gattgcgact cgccggaaaa gatgatttca
      241 gaaaatccgg atatcagaat ggcagaggct gttttgagag caggaataaa aactgaataa
      301 ttaaatttag cacagcaaat aaaatttaat ccttaaccgg agggatttct gcaccctcag
      361 aaca
//
LOCUS       DAJTNN010000449          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJTNN010000449 DAJTNN010000000
VERSION     DAJTNN010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.462"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..334
                     /locus_tag="OO150_004387"
     CDS             <1..334
                     /locus_tag="OO150_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5518278.1"
                     /translation="RIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLAQIDA
                     VLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNPKYKV
                     EIKIVAAV"
BASE COUNT           98 a           85 c          103 g           78 t
ORIGIN      
        1 tcgtatcgat gctgaagccc gctggtctga tgtagtaatc cacaacaaca cgctctacta
       61 cactggtgta ccggaaaacc tcgacgccga tgcttttgag caaaccgcca acacgctggc
      121 gcagattgac gccgtgctgg aaaaacaggg cagcaataaa tcgagcattc tggatgccac
      181 cattttcctg gctgataaaa acgacttcgc ggcgatgaat aaagcgtggg atgcctgggt
      241 tgtggcgggt catgcgccgg tgcgctgcac ggtgcaagcg gggctgatga acccgaagta
      301 taaagttgaa attaagattg tggctgcggt gtaagcttta tcgaagcaaa ataagtcaga
      361 cgat
//
LOCUS       DAJTNN010000450          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJTNN010000450 DAJTNN010000000
VERSION     DAJTNN010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.463"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>361
                     /locus_tag="OO150_004388"
     CDS             <1..>361
                     /locus_tag="OO150_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5518279.1"
                     /translation="KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHL
                     KGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPR
                     VVKERPWKYPTAPKKSQS"
BASE COUNT          108 a           71 c          111 g           71 t
ORIGIN      
        1 aaacagacga tgcaactgag caggctgacg ctgagaagta aaaagccgga gcttgtggag
       61 caagagctgt ggggtgtctt actggcttat aatctggtga gatatcagat gattaaaatg
      121 gcggaacatc tgaaaggtta ctggccgaat caactgagtt tctcagaatc atgcggaatg
      181 gtgatgagaa tgctgatgac attgcagggc gcttcaccgg gacgtatacc ggagctgatg
      241 cgcgatcttg caagtatggg acaacttgtg aaattaccga cgagaagggg aagggccttc
      301 ccgagagtgg taaaggagag gccctggaaa taccccacag ccccgaaaaa gagccagtca
      361 g
//
LOCUS       DAJTNN010000451          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJTNN010000451 DAJTNN010000000
VERSION     DAJTNN010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.464"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>361
                     /locus_tag="OO150_004389"
     CDS             <1..>361
                     /locus_tag="OO150_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5518280.1"
                     /translation="QIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQL
                     CKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVE
                     QRVLAHPLYPVLTSMPGV"
BASE COUNT           77 a          116 c           97 g           71 t
ORIGIN      
        1 cagatacatc cggcactgga gcgcgttctc ggtccgagac ttgagcaccc ggcggtactc
       61 gatcttctcc agcgatatcc ctcaccagaa aaactcgctt cgctgggtga gaagaagctg
      121 gcagcccagc tctgcaaact tgcgcctcgt ctgggtaaac gccttgcagc agacatagct
      181 caggcactgg ccgaacaaac cgtcgtcgtt cccggcacga atgccgctgc cgtagtactg
      241 ccacgtctgg cactccagct catcacgctg cgtaagcaaa gagacgaggt ggcgcttgag
      301 gtagaacagc gagttcttgc tcaccctctt tacccggtcc tgaccagtat gcccggagtc
      361 g
//
LOCUS       DAJTNN010000452          356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJTNN010000452 DAJTNN010000000
VERSION     DAJTNN010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.465"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            68..>356
                     /locus_tag="OO150_004390"
     CDS             68..>356
                     /locus_tag="OO150_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5518281.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTG"
BASE COUNT          103 a           87 c           86 g           80 t
ORIGIN      
        1 acaccggatt cagaaccaac aacctctgta ttacaaggca cagcgcagtg ccattaagga
       61 taaaaaaatg aaaagagcgc ctcttataac aggacttttg ttgatatcca catcctgcgc
      121 ttatgcctcc tcaggagggt gtggagccga cagcactagc ggtgcgacaa attacagcag
      181 tgtggttgat gatgttacgg tgaaccagac agataacgtg acaggacggg agtttacctc
      241 tgcaacgcta agtagcacta actggcaata cgcctgttcc tgctctgcgg gtaaggcagt
      301 taaacttgtc tatatggtca gccccgtact taccaccact ggacatcaga caggat
//
LOCUS       DAJTNN010000453          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.466, whole genome shotgun sequence.
ACCESSION   DAJTNN010000453 DAJTNN010000000
VERSION     DAJTNN010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.466"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>353
                     /locus_tag="OO150_004391"
     CDS             <1..>353
                     /locus_tag="OO150_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5518282.1"
                     /translation="TLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQAGNTFAKESGA
                     SVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALV
                     VKKTISKPEDLIGKR"
BASE COUNT           84 a           96 c          104 g           69 t
ORIGIN      
        1 acacttcttg ccgcactggc attcatcgct tttcaggcac aggcggtgaa cgtcaccgtg
       61 gcgtatcaaa cctcagccga accggcgaaa gtggctcagg ccggcaacac ctttgctaaa
      121 gagagcgggg cgagcgtgga ctggcgtaag tttgacagcg gagccagcat cgtgcgggcg
      181 ctggcttctg gcgacgtgca aatcggcaac ctcggttcca gcccattagc ggttgcagcc
      241 agccaacagg tgccgattga agtcttcttg ctggcgtcaa aactgggtaa ctccgaagcg
      301 ctggtggtaa agaaaactat cagcaaaccc gaagatttga ttggcaaacg cat
//
LOCUS       DAJTNN010000454          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.467, whole genome shotgun sequence.
ACCESSION   DAJTNN010000454 DAJTNN010000000
VERSION     DAJTNN010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.467"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..274
                     /locus_tag="OO150_004392"
     CDS             <1..274
                     /locus_tag="OO150_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="oxidoreductase, Fe-S subunit; terminal electron
                     transfer protein for the reduction of DMSO; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HCR5518283.1"
                     /translation="YNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELR
                     KKHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            276..>353
                     /locus_tag="OO150_004393"
     CDS             276..>353
                     /locus_tag="OO150_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5518284.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIV"
BASE COUNT           85 a           81 c          105 g           82 t
ORIGIN      
        1 atataacgaa acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga
       61 gggtaaaaag ccgatctgtg ttgaatcttg tccgctgcgg gcgctggatt tcggccctat
      121 cgacgagctg cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca
      181 ctttaccaaa ccgaatattg tgatcaaacc caatgccaat agccgcccga ccggggatac
      241 cactggctat ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc
      301 cgctgatgat cttcacggtc ttcgggcaat gtgtagcagg tggttttatc gtt
//
LOCUS       DAJTNN010000455          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.468, whole genome shotgun sequence.
ACCESSION   DAJTNN010000455 DAJTNN010000000
VERSION     DAJTNN010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.468"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>351
                     /locus_tag="OO150_004394"
     CDS             <1..>351
                     /locus_tag="OO150_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR5518285.1"
                     /translation="IMRKQIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMA
                     SDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPE
                     AARVVFTSGVPLVM"
BASE COUNT           82 a           93 c           92 g           84 t
ORIGIN      
        1 tattatgcgt aaacaaatcg ttgccgataa tattcacggt gaaaccggac tggatggtcc
       61 ggtattcgag tcgctgaccc gccaggcaga aagtacccac gcggtgaaat acatcattga
      121 taccctgatg gcaagcgatg gcgatatcac tctggtgccg gttggtccgc tttcaaatat
      181 cgcggtggca atgcgtatgc aacccgcgat cctgcccaaa atccgtgaaa ttgtgctaat
      241 gggcggcgct tacggtacag gcaacttcac gccatctgcc gagttcaaca tctttgctga
      301 tccggaagca gcccgcgtgg tgttcacctc cggcgttcca ttagtgatga t
//
LOCUS       DAJTNN010000456          349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.469, whole genome shotgun sequence.
ACCESSION   DAJTNN010000456 DAJTNN010000000
VERSION     DAJTNN010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.469"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(62..145)
                     /gene="yohP"
                     /locus_tag="OO150_004395"
     CDS             complement(62..145)
                     /gene="yohP"
                     /locus_tag="OO150_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5518286.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT          117 a           72 c           53 g          107 t
ORIGIN      
        1 caaatcaagt acacaaaata tctagttcct gacctgacaa cttatatgcc atatctgatt
       61 tttaaaatat catcttaaat acgccagtca ccaccagtag cccaatcagg aaaataatca
      121 atacagccca gagtataatt ttcattggcg ttcctttatt tgtgagtgtt gaataagtat
      181 aggaaaaaac gaagcccttt gccgttatcc ataaacgatt aagataaatc ggaagatatc
      241 attcacttag tctatatttt tctgggtatg tcgttttatt gctactgatg tattgccacg
      301 cgaaactaaa aaatatatcg caacacacgg taatctccgc gcaccgttt
//
LOCUS       DAJTNN010000457          346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.470, whole genome shotgun sequence.
ACCESSION   DAJTNN010000457 DAJTNN010000000
VERSION     DAJTNN010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.470"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..69)
                     /locus_tag="OO150_004396"
     CDS             complement(<1..69)
                     /locus_tag="OO150_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR5518287.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYW"
BASE COUNT          115 a           79 c           76 g           76 t
ORIGIN      
        1 ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cccgttgaaa ttttgtccat
       61 ggatttcata gcctcacctc cgcaaataac ggatggcgta gttttacact gagaaatgaa
      121 agggatttga aaagaaaaaa acgcaaaagc gggcgaaacg atatatacag taaggaaagc
      181 actctatcca acaaaccacc cacagttaat cggaataaaa gcagagtgct tatgaatgat
      241 cgcacgcccg aaggttagta tttctgcaca gcaattttgc aaaaaaaggc gatcattcat
      301 aacttaaacg tctttcagtc actccgggat ttcccatcat cgcaga
//
LOCUS       DAJTNN010000458          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.471, whole genome shotgun sequence.
ACCESSION   DAJTNN010000458 DAJTNN010000000
VERSION     DAJTNN010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.471"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..327
                     /locus_tag="OO150_004397"
     CDS             <1..327
                     /locus_tag="OO150_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518288.1"
                     /translation="GCFKKEYSIVSHMLVTDTITEATLYENGLYTRHIRLSRPVSDSK
                     YPLPPGSQLIKNMTLRLRLQDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKM
                     LQINHA"
BASE COUNT          108 a           88 c           67 g           77 t
ORIGIN      
        1 gggtgcttta aaaaagaata cagcattgtc tcccacatgc tggtcactga caccataaca
       61 gaggccaccc tgtacgaaaa cgggctgtat acgcgccaca tcagactcag tcgcccagta
      121 tctgacagca aatacccctt gccacccgga tctcagctca ttaaaaatat gaccttacga
      181 ctgcgcctgc aggatcagca ggaagaaaca tccgtgacat tatttcaggg aaaaatgagt
      241 accgacggaa ataactatta catcatcaaa ggtaaagtaa gctcagtact tctgttatta
      301 aaaatgctgc agattaatca cgcctgatct ctgtcccggt
//
LOCUS       DAJTNN010000459          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.472, whole genome shotgun sequence.
ACCESSION   DAJTNN010000459 DAJTNN010000000
VERSION     DAJTNN010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.472"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(1..58)
                     /locus_tag="OO150_004398"
     tRNA            complement(1..58)
                     /locus_tag="OO150_004398"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31..33),aa:Thr,seq:tgt)
     gene            complement(69..145)
                     /locus_tag="OO150_004399"
     tRNA            complement(69..145)
                     /locus_tag="OO150_004399"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(109..111),aa:Arg,seq:tct)
     gene            complement(147..223)
                     /locus_tag="OO150_004400"
     tRNA            complement(147..223)
                     /locus_tag="OO150_004400"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(187..189),aa:Ile,seq:cat)
BASE COUNT           83 a           93 c           84 g           77 t
ORIGIN      
        1 taccggaatc gaactggtga cctactgatt acaagtcagt tgctctgcct gctgagctaa
       61 gtcggcgctg gcccaccacc gaggactcga acctcgcacc gtcaacttag aaggttgatg
      121 ctctatccgg atgagctagt ggtggttggt ggcccttgct ggacttgaac cagcgacctg
      181 gcgattatga gtcgctcgct ctgaccaact gagctaaagg gccggaggca gaataataac
      241 catatgtcat cacatctgca aacccatctg accaccagca cgtttaacgt cctgtgccgt
      301 ttttcaggta taaaaaaacc cgcataaagc gggctct
//
LOCUS       DAJTNN010000460          329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.473, whole genome shotgun sequence.
ACCESSION   DAJTNN010000460 DAJTNN010000000
VERSION     DAJTNN010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.473"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..>329)
                     /gene="speG"
                     /locus_tag="OO150_004401"
     CDS             complement(<1..>329)
                     /gene="speG"
                     /locus_tag="OO150_004401"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCR5518289.1"
                     /translation="TTSHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIIS
                     PEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHE
                     FFINGQY"
BASE COUNT           87 a           82 c           67 g           93 t
ORIGIN      
        1 gatattgacc attaataaaa aactcatgca tcaattcacc ttcaaccgta aagccaagct
       61 tgcggtaaat gtgaatcgct ttttcattct ctttatcgac gatcagatac agcttataga
      121 gattgagaac ggtgaagcca tagtccattg ctaatttggc ggcacgggtt gccagacctt
      181 tcccctgata ctccggggag ataattatct gaaattctgc ccggcgatgt acatggttaa
      241 tttccaccag ctccaccaga ccggcttttt cgccgtcaca ttccaccaca aagcgtcgtt
      301 cgctctgatc gtgaatatgt gaagtggtc
//
LOCUS       DAJTNN010000461          326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.475, whole genome shotgun sequence.
ACCESSION   DAJTNN010000461 DAJTNN010000000
VERSION     DAJTNN010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.475"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(189..296)
                     /locus_tag="OO150_004402"
     CDS             complement(189..296)
                     /locus_tag="OO150_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5518290.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWHNRK"
BASE COUNT           73 a           87 c           81 g           85 t
ORIGIN      
        1 aagagtgcat tccggttttt caacagctgt tacagtcgtt tcatgagtgc tctggatgat
       61 gcttccagct cgggttgcca atatttactt gtggaagaga taaagacaaa aatggccgca
      121 ggctgttacc cctgcggccg gtttcgggcg catattgcca tcacggcagc ctgacgcccg
      181 ttttcacctt acttccggtt atgccaccag ccgacaatcg ctgcggtaat aatccccgcc
      241 aggatcggtg ctgccaggtc gtgccagaaa atcatggcaa acttcgcgag cgtcatatag
      301 ccgccttgtt gtaatgacaa catttt
//
LOCUS       DAJTNN010000462          326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.476, whole genome shotgun sequence.
ACCESSION   DAJTNN010000462 DAJTNN010000000
VERSION     DAJTNN010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.476"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>326
                     /locus_tag="OO150_004403"
     CDS             <1..>326
                     /locus_tag="OO150_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5518291.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEA"
BASE COUNT           74 a           85 c           97 g           70 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagc
//
LOCUS       DAJTNN010000463          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.477, whole genome shotgun sequence.
ACCESSION   DAJTNN010000463 DAJTNN010000000
VERSION     DAJTNN010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.477"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..>325
                     /locus_tag="OO150_004404"
     CDS             <1..>325
                     /locus_tag="OO150_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518292.1"
                     /translation="LRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQ
                     SGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERL
                     VSTART"
BASE COUNT           66 a           91 c          117 g           51 t
ORIGIN      
        1 ttgcgccagc gcctgttccg ctgcgtgagt atccgtgaga acgatgacgg cacgtatgcc
       61 atcaccgccg tgcagcatgt accggaaaaa gaggccatcg tggataacgg ggcgcacttt
      121 gacggcgacc agagcggcac ggtgaatggt gtcacgccgc cagcagtgca gcatctgacc
      181 gccgaagtca ctgcagacag cggggaatat caggtgctgg cgcgatggga cacgccgaag
      241 gtggtgaagg gcgtgagctt cctgcttcgc ctgaccgtgg cagcggatga cggcagtgag
      301 cggctggtca gcacggcccg gacgg
//
LOCUS       DAJTNN010000464          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.478, whole genome shotgun sequence.
ACCESSION   DAJTNN010000464 DAJTNN010000000
VERSION     DAJTNN010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.478"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..310
                     /locus_tag="OO150_004405"
     CDS             <1..310
                     /locus_tag="OO150_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5518293.1"
                     /translation="YFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGK
                     ENVKAVKLTCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPSNCGKTFVIDKVGY
                     "
BASE COUNT          100 a           75 c           72 g           76 t
ORIGIN      
        1 atacttcggt acgatggtac gcctgaatca agaaattaaa gagagcgaag ccggaaaatt
       61 ccttgcggat aattacggta aaacagtgag ccgccgtgac tttgacgccg cctttgccaa
      121 aagctgggga aaagagaacg tgaaagcagt taagctaacg tgccagggta accctgcgta
      181 tttgactgaa attcagatct cgatcaaagc tgacgccatc aacgctccgc tttctgcaaa
      241 ctcattcttg ccacaaccac acccaagcaa ctgtggcaaa acctttgtga ttgataaagt
      301 gggttattaa tcatctgatt tac
//
LOCUS       DAJTNN010000465          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.479, whole genome shotgun sequence.
ACCESSION   DAJTNN010000465 DAJTNN010000000
VERSION     DAJTNN010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.479"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            52..>321
                     /locus_tag="OO150_004406"
     CDS             52..>321
                     /locus_tag="OO150_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5518294.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEILKKATAL"
BASE COUNT          118 a           73 c           72 g           58 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct c
//
LOCUS       DAJTNN010000466          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.denovo.480, whole genome shotgun sequence.
ACCESSION   DAJTNN010000466 DAJTNN010000000
VERSION     DAJTNN010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.denovo.480"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            1..304
                     /locus_tag="OO150_004407"
                     /pseudo
     CDS             1..304
                     /locus_tag="OO150_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           66 a           70 c           88 g           80 t
ORIGIN      
        1 gtttttgggc gagtgtatct ggcgcatgag ggcattaacg cgcaaatcag cgtacctgcg
       61 agcaatgttg aaacatttcg cgcgcagctc tgccttcgac ccggctttag agggtttacg
      121 cctgaatatc gcgttggatg atgacgggaa atccttctgg gtactgcgca tgaaggtacg
      181 tgatcgcatt gttgccgacg gtattgacga tcctcacttt gatgccagca atgttggtga
      241 gtatctgcaa gcggcggaag tgaacgccat gcttgacgat cccgatgcac tatttatcga
      301 catg
//
LOCUS       DAJTNN010000467         7922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.guided.177, whole genome shotgun sequence.
ACCESSION   DAJTNN010000467 DAJTNN010000000
VERSION     DAJTNN010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.guided.177"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..2234)
                     /locus_tag="OO150_004408"
     CDS             complement(<1..2234)
                     /locus_tag="OO150_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5518295.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDL"
     gene            complement(2237..2797)
                     /locus_tag="OO150_004409"
     CDS             complement(2237..2797)
                     /locus_tag="OO150_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5518296.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(2923..3273)
                     /locus_tag="OO150_004410"
     CDS             complement(2923..3273)
                     /locus_tag="OO150_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5518297.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(3476..4489)
                     /gene="intI1"
                     /locus_tag="OO150_004411"
     CDS             complement(3476..4489)
                     /gene="intI1"
                     /locus_tag="OO150_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5518298.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            4699..5529
                     /locus_tag="OO150_004412"
     CDS             4699..5529
                     /locus_tag="OO150_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5518299.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            5642..6433
                     /gene="aadA1"
                     /locus_tag="OO150_004413"
     CDS             5642..6433
                     /gene="aadA1"
                     /locus_tag="OO150_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5518300.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            6509..7006
                     /locus_tag="OO150_004414"
     CDS             6509..7006
                     /locus_tag="OO150_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518301.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            7067..7438
                     /locus_tag="OO150_004415"
     CDS             7067..7438
                     /locus_tag="OO150_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518302.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
BASE COUNT         1839 a         2110 c         2231 g         1742 t
ORIGIN      
        1 aggtcggatt cgtggtacag caggccgtcg agcacgtagg ttgagtcgcg caggccgaca
       61 ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga tgtgggtgta gaaagtccgt
      121 cctgggctgc tgccatattt tgggttgatg tgccccgtgc tctttgcctt gctagcggtt
      181 cggaaattct gtccgtccga tgatgatgtg gtgccatcgc cccagtgccc ggcaaaggga
      241 tgccgaaact gagcgttgac cagttcagcc aacgctgtcg agtacgtttc gtcgcgggta
      301 tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc cggggcagga ctcggccatc
      361 ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg tcaacaacag gttcttgtcc
      421 ttggccagat cgcccgattt caagtgcgtg aagtgccggg tgaagcccgt ccactcatcg
      481 acttcgagca gcagttcggt gatcttgacg tgcggcagga ccatggctgt ctggtctatc
      541 agcgcctgcg cggtgtcggg caccgccgca tccagcggcg tgatcttcaa gcccgactcg
      601 gtgatgatgg catccggcag gtcgttggct gccgccatgc ggttgacggt ggcaagttgt
      661 gcttccagca gcgtcagccg ctcatgcaga tattgttcgc agtcggtggc cacggccagc
      721 ggcaattcgc tggactgctt gaggctggtg aacttctcgg gcggtaccag gtagtcctcg
      781 aagtccttga actggcgtga accctgcacc cagatgtcgc ccgagcgcag ggagttcttc
      841 aactcggaca gcgcgcacag ttcgtagtag cgccggtcga tgccggcgtc ggtcatcacc
      901 agtttctgcc agcgcggctt gatgaagccg gtcggtgcat cggctggcag cttgcgggcg
      961 ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa gcacgttttt ggcggcgggc
     1021 gcggcccgca gcttgagcac ggcaaggaat tccggtgcat agcggcgcag ggtggcgtag
     1081 ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt gcgcgagctt ctgcgcctcg
     1141 gtgacgctct cggcaaagga atcccaggac atgacggcct cgatggcggc aaacgcatcg
     1201 cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc gcccgtacag acgtaccttg
     1261 gcgttgatgg ccttgcctga cgcctggaac tgctgctgat gcttattctt ggcagcgtta
     1321 aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt cgtcggtgac ggtggccatg
     1381 ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt gcggctcgaa tttggccagg
     1441 tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga gcaggcggtt ctggtgaacc
     1501 agccgctcga tgccggtagg cagatcgagt gcctgccatg ccttgaggcg ttcgatgtgt
     1561 tccagcatat gccgcgaatt tggcttggcc ggagactggc gcaaccaagc caaccaggtc
     1621 gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc ggcggcgatg cgcgtccgcc
     1681 agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac gggtgatcgc ctcggcactc
     1741 gcccgctcga cggcgttgag ggcgggcaga atgaccgact gccgccgcag gtgcccgatc
     1801 aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg ccagctcggt cagcatctgg
     1861 acggcctgcc ggtaatggct catggtgaag ggccggaaac cgaacacggt ttgcagctcg
     1921 ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact cgttccagct ttcgacgccg
     1981 accttgagct ggtcggcgac cagcttcaac aagggcggga acggtagttc atcgacgccc
     2041 aggatgacgc cgggaaagcg caggtaacag agctgcaccg cgaagcccag ccgattggct
     2101 ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt tgaatgtgta atgtcggatc
     2161 aggtcgtcct tggagtccgg caacgccagc aggctttccc gctcggcggc ggacaggatg
     2221 gaacgacgtg gcatatttac tgatccgttc tcaagtattg atacagggtt tcgcgactga
     2281 ttccgaattc acgagcaagc ttggtctttt gctcgccagc ctcgacacgt tggcgcagtt
     2341 cggcaatacg ctcagacgac agggatttct tcctgccacg gtaagccccg cgttgcttgg
     2401 cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc gcgctcgaac tcggcgaacg
     2461 cgcccatcac cgagagcatc aggttcgcca tcggagagtc ttcgccagta aaactgaggt
     2521 gttccttgac gaattcgata tgcacgccgc gttgtgtcag cgtttgcacg atccggcgca
     2581 aatcatcgag attgcgcgcc aggcgatcca tgctatgcac caccacggtg tcgccggtgc
     2641 gggcgaagct tatcagcgct tccagttgcg gacgcttgac atccttgccg gatgccttgt
     2701 cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg ttccgggttc tggtcgaagg
     2761 tgctgaccct gatataccca atgcgctgtc cagtcatgga attccctgca aaatgtcagg
     2821 gaagactcta tgaccttcaa cgagatatgt caataaattc aaaattcaat cctatcctga
     2881 cgcaatttac acatggcatc tgacatcagg ttagggtatg cctcaacctg acggcggcga
     2941 atcacaagcg tccggtttga cgctggtttc ggtcgcagtg ctggcccgcg cctggaagcg
     3001 ctgatagcac tccagcccgc agaaatgctc cacgtactcg gccccttccg gcgtgaaggc
     3061 ggcatcgagc gggatttcct tgcagcacac gcagcaactg gtgctcggtt cattggcgtt
     3121 catggtggtg ttcctccatt ggttgacgaa gccgacgaag gccgccgccg gcatcggcct
     3181 ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc cgcaaccacg caaggcagtc
     3241 gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt ttcagaagac ggctgcactg
     3301 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatccatcag gcaacgacgg
     3361 gctgctgccg gccatcagcg gacgcaggga ggactttccg caaccggccg ttcgatgcgg
     3421 caccgatggc cttcgcgcag gggtagtgaa tccgccagga ttgacttgcg ctgccctacc
     3481 tctcactagt gaggggcggc agcgcatcaa gcggtgagcg cactccggca ccgccaactt
     3541 tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga atggccgagc agatcctgca
     3601 cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc gaacgagtgg cggagggtgt
     3661 gcggtgtggc gggcttcgtg atgcctgctt gttctacggc acgtttgaag gcgcgctgaa
     3721 aggtctggtc atacatgtga tggcgacgca cgacaccgct ccgtggatcg gtcgaatgcg
     3781 tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc ggcgcgcgga tacttccgct
     3841 caagggcgtc gggaagcgca acgccgctgc ggccctcggc ctggtccttc agccaccatg
     3901 cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg
     3961 cccgatcctt ggagcccttg ccctcccgca cgatgatcgt gccgtgatcg aaatccagat
     4021 ccttgacccg cagttgcaaa ccctcactga tccgcatgcc cgttccatac agaagctggg
     4081 cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg aaccacttca tccggggtca
     4141 gcaccaccgg caagcgccgc gacggccgag gtcttccgat ctcctgaagc cagggcagat
     4201 ccgtgcacag caccttgccg tagaagaaca gcaaggccgc caatgcctga cgatgcgtgg
     4261 agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc
     4321 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     4381 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     4441 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     4501 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     4561 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     4621 aaacaaagtt gggcgaaccc ggagcctcat taattgttag ccgttaaaat taagcccttt
     4681 accaaaccaa tacttattat gaaaaacaca atacatatca acttcgctat ttttttaata
     4741 attgcaaata ttatctacag cagcgccagt gcatcaacag atatctctac tgttgcatct
     4801 ccattatttg aaggaactga aggttgtttt ttactttacg atgcatccac aaacgctgaa
     4861 attgctcaat tcaataaagc aaagtgtgca acgcaaatgg caccagattc aactttcaag
     4921 atcgcattat cacttatggc atttgatgcg gaaataatag atcagaaaac catattcaaa
     4981 tgggataaaa cccccaaagg aatggagatc tggaacagca atcatacacc aaagacgtgg
     5041 atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc aaaaaattgg attaaataaa
     5101 atcaagaatt atctcaaaga ttttgattat ggaaatcaag acttctctgg agataaagaa
     5161 agaaacaacg gattaacaga agcatggctc gaaagtagct taaaaatttc accagaagaa
     5221 caaattcaat tcctgcgtaa aattattaat cacaatctcc cagttaaaaa ctcagccata
     5281 gaaaacacca tagagaacat gtatctacaa gatctggata atagtacaaa actgtatggg
     5341 aaaactggtg caggattcac agcaaataga accttacaaa acggatggtt tgaagggttt
     5401 attataagca aatcaggaca taaatatgtt tttgtgtccg cacttacagg aaacttgggg
     5461 tcgaatttaa catcaagcat aaaagccaag aaaaatgcga tcaccattct aaacacacta
     5521 aatttataaa aaatctaatg gcaaaatcgc ccaacccttc aatcaagtcg ggacggccaa
     5581 aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc ttatttcaaa cgttaaacat
     5641 catgagggaa gtggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     5701 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     5761 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     5821 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     5881 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     5941 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     6001 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     6061 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     6121 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     6181 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     6241 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     6301 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     6361 ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg aaaggcgaga tcaccaaggt
     6421 agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     6481 ctcaagcgtt agagagctgg ggaagactat gcgcgatctg ttgaaggtgg ttctaagcct
     6541 cgtacttgcg atggcatcgg ggcaggcact tgctgacctg ccaattgttt tagtggatga
     6601 agctcgtctt ccctatgact actccccatc caactacgac atttctccaa gcaactacga
     6661 caactccata agcaattacg acaatagtcc atcaaattac gacaactctg agagcaacta
     6721 cgataatagt tcatccaatt acgacaatag tcgcaacgga aatcgtaggc ttatatatag
     6781 cgcaaatggg tctcgcactt tcgccggcta ctacgtcatt gccaacaatg ggacaacgaa
     6841 cttcttttcc acatctggca aaaggatgtt ctacacccca aaaggggggc gcggcgtcta
     6901 tggcggcaaa gatgggagct tctgcggggc attggtcgtc ataaatggcc aattttcgct
     6961 tgccctgaca gataacggcc tgaagatcat gtatctaagc aactagcctg ctctctaata
     7021 aaatgttagg cctcaacatc tagtcgcaag ctgaggggaa ccactagtgt catacgaacc
     7081 tccaagagac ggttacacaa acgggtacat tgttgatgtc atgtatgaca atcgcccaag
     7141 taagtatcca gctgtgttca gaacgtacgt ccgaattcca gactatgtcg aactagataa
     7201 cgcggaggag ctggccgacg caatcattcg agaggttgag ttaaaggagg catggctcac
     7261 aagggtcttg gggaactcag ccgatcctga cgcttctgtg ggcaagtggg accggcgcct
     7321 agcgcagtgc ggtggtattg atgagatgtc aagtggggtg caggcaacat acttcagtcc
     7381 aaacggtcag ctggtcggct tagtggcata cattgatgcc gaatggatgg atgaatagct
     7441 ctgacgttac gattcaggtg agacaaccag atgggcattg cgtacaagct cagttgccac
     7501 cgatgtggct acgagtctga tcttctttac ctaggccaag gtatggcgat gcttcctgag
     7561 caaatagtcg gaacgtgcac atgctgcgac aatctaacga cgatttctat aaatgaaagc
     7621 tacggagtat gctcactttg tggggctaag gatcgtgtga tatttacaag ctatcagcat
     7681 gaggcaaggc caagaaatcc ctgctatcta gagagaagtt tcaaatatca atgcccaaga
     7741 tgccatgcct tcagtatgga gcctccggat ctaccggaga tgctttggga ctaatgctcc
     7801 gctttgcctc gggtatccgt tcgacctatg tttaagagga aacgcacgtg tctgccgcca
     7861 aagctcttgc ctatgcactt tgtgacaagt tggaaagcaa aaaagttgat gaagtcgtac
     7921 ga
//
LOCUS       DAJTNN010000468         7919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.guided.178, whole genome shotgun sequence.
ACCESSION   DAJTNN010000468 DAJTNN010000000
VERSION     DAJTNN010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.guided.178"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..2234)
                     /locus_tag="OO150_004416"
     CDS             complement(<1..2234)
                     /locus_tag="OO150_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5518303.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDL"
     gene            complement(2237..2797)
                     /locus_tag="OO150_004417"
     CDS             complement(2237..2797)
                     /locus_tag="OO150_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5518304.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(2923..3273)
                     /locus_tag="OO150_004418"
     CDS             complement(2923..3273)
                     /locus_tag="OO150_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5518305.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(3476..4489)
                     /gene="intI1"
                     /locus_tag="OO150_004419"
     CDS             complement(3476..4489)
                     /gene="intI1"
                     /locus_tag="OO150_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5518306.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            4699..5529
                     /locus_tag="OO150_004420"
     CDS             4699..5529
                     /locus_tag="OO150_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5518307.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            5642..6430
                     /gene="aadA1"
                     /locus_tag="OO150_004421"
     CDS             5642..6430
                     /gene="aadA1"
                     /locus_tag="OO150_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5518308.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            6506..7003
                     /locus_tag="OO150_004422"
     CDS             6506..7003
                     /locus_tag="OO150_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518309.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            7064..7435
                     /locus_tag="OO150_004423"
     CDS             7064..7435
                     /locus_tag="OO150_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518310.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
BASE COUNT         1836 a         2110 c         2231 g         1742 t
ORIGIN      
        1 aggtcggatt cgtggtacag caggccgtcg agcacgtagg ttgagtcgcg caggccgaca
       61 ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga tgtgggtgta gaaagtccgt
      121 cctgggctgc tgccatattt tgggttgatg tgccccgtgc tctttgcctt gctagcggtt
      181 cggaaattct gtccgtccga tgatgatgtg gtgccatcgc cccagtgccc ggcaaaggga
      241 tgccgaaact gagcgttgac cagttcagcc aacgctgtcg agtacgtttc gtcgcgggta
      301 tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc cggggcagga ctcggccatc
      361 ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg tcaacaacag gttcttgtcc
      421 ttggccagat cgcccgattt caagtgcgtg aagtgccggg tgaagcccgt ccactcatcg
      481 acttcgagca gcagttcggt gatcttgacg tgcggcagga ccatggctgt ctggtctatc
      541 agcgcctgcg cggtgtcggg caccgccgca tccagcggcg tgatcttcaa gcccgactcg
      601 gtgatgatgg catccggcag gtcgttggct gccgccatgc ggttgacggt ggcaagttgt
      661 gcttccagca gcgtcagccg ctcatgcaga tattgttcgc agtcggtggc cacggccagc
      721 ggcaattcgc tggactgctt gaggctggtg aacttctcgg gcggtaccag gtagtcctcg
      781 aagtccttga actggcgtga accctgcacc cagatgtcgc ccgagcgcag ggagttcttc
      841 aactcggaca gcgcgcacag ttcgtagtag cgccggtcga tgccggcgtc ggtcatcacc
      901 agtttctgcc agcgcggctt gatgaagccg gtcggtgcat cggctggcag cttgcgggcg
      961 ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa gcacgttttt ggcggcgggc
     1021 gcggcccgca gcttgagcac ggcaaggaat tccggtgcat agcggcgcag ggtggcgtag
     1081 ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt gcgcgagctt ctgcgcctcg
     1141 gtgacgctct cggcaaagga atcccaggac atgacggcct cgatggcggc aaacgcatcg
     1201 cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc gcccgtacag acgtaccttg
     1261 gcgttgatgg ccttgcctga cgcctggaac tgctgctgat gcttattctt ggcagcgtta
     1321 aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt cgtcggtgac ggtggccatg
     1381 ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt gcggctcgaa tttggccagg
     1441 tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga gcaggcggtt ctggtgaacc
     1501 agccgctcga tgccggtagg cagatcgagt gcctgccatg ccttgaggcg ttcgatgtgt
     1561 tccagcatat gccgcgaatt tggcttggcc ggagactggc gcaaccaagc caaccaggtc
     1621 gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc ggcggcgatg cgcgtccgcc
     1681 agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac gggtgatcgc ctcggcactc
     1741 gcccgctcga cggcgttgag ggcgggcaga atgaccgact gccgccgcag gtgcccgatc
     1801 aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg ccagctcggt cagcatctgg
     1861 acggcctgcc ggtaatggct catggtgaag ggccggaaac cgaacacggt ttgcagctcg
     1921 ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact cgttccagct ttcgacgccg
     1981 accttgagct ggtcggcgac cagcttcaac aagggcggga acggtagttc atcgacgccc
     2041 aggatgacgc cgggaaagcg caggtaacag agctgcaccg cgaagcccag ccgattggct
     2101 ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt tgaatgtgta atgtcggatc
     2161 aggtcgtcct tggagtccgg caacgccagc aggctttccc gctcggcggc ggacaggatg
     2221 gaacgacgtg gcatatttac tgatccgttc tcaagtattg atacagggtt tcgcgactga
     2281 ttccgaattc acgagcaagc ttggtctttt gctcgccagc ctcgacacgt tggcgcagtt
     2341 cggcaatacg ctcagacgac agggatttct tcctgccacg gtaagccccg cgttgcttgg
     2401 cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc gcgctcgaac tcggcgaacg
     2461 cgcccatcac cgagagcatc aggttcgcca tcggagagtc ttcgccagta aaactgaggt
     2521 gttccttgac gaattcgata tgcacgccgc gttgtgtcag cgtttgcacg atccggcgca
     2581 aatcatcgag attgcgcgcc aggcgatcca tgctatgcac caccacggtg tcgccggtgc
     2641 gggcgaagct tatcagcgct tccagttgcg gacgcttgac atccttgccg gatgccttgt
     2701 cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg ttccgggttc tggtcgaagg
     2761 tgctgaccct gatataccca atgcgctgtc cagtcatgga attccctgca aaatgtcagg
     2821 gaagactcta tgaccttcaa cgagatatgt caataaattc aaaattcaat cctatcctga
     2881 cgcaatttac acatggcatc tgacatcagg ttagggtatg cctcaacctg acggcggcga
     2941 atcacaagcg tccggtttga cgctggtttc ggtcgcagtg ctggcccgcg cctggaagcg
     3001 ctgatagcac tccagcccgc agaaatgctc cacgtactcg gccccttccg gcgtgaaggc
     3061 ggcatcgagc gggatttcct tgcagcacac gcagcaactg gtgctcggtt cattggcgtt
     3121 catggtggtg ttcctccatt ggttgacgaa gccgacgaag gccgccgccg gcatcggcct
     3181 ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc cgcaaccacg caaggcagtc
     3241 gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt ttcagaagac ggctgcactg
     3301 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatccatcag gcaacgacgg
     3361 gctgctgccg gccatcagcg gacgcaggga ggactttccg caaccggccg ttcgatgcgg
     3421 caccgatggc cttcgcgcag gggtagtgaa tccgccagga ttgacttgcg ctgccctacc
     3481 tctcactagt gaggggcggc agcgcatcaa gcggtgagcg cactccggca ccgccaactt
     3541 tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga atggccgagc agatcctgca
     3601 cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc gaacgagtgg cggagggtgt
     3661 gcggtgtggc gggcttcgtg atgcctgctt gttctacggc acgtttgaag gcgcgctgaa
     3721 aggtctggtc atacatgtga tggcgacgca cgacaccgct ccgtggatcg gtcgaatgcg
     3781 tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc ggcgcgcgga tacttccgct
     3841 caagggcgtc gggaagcgca acgccgctgc ggccctcggc ctggtccttc agccaccatg
     3901 cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg
     3961 cccgatcctt ggagcccttg ccctcccgca cgatgatcgt gccgtgatcg aaatccagat
     4021 ccttgacccg cagttgcaaa ccctcactga tccgcatgcc cgttccatac agaagctggg
     4081 cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg aaccacttca tccggggtca
     4141 gcaccaccgg caagcgccgc gacggccgag gtcttccgat ctcctgaagc cagggcagat
     4201 ccgtgcacag caccttgccg tagaagaaca gcaaggccgc caatgcctga cgatgcgtgg
     4261 agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc
     4321 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     4381 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     4441 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     4501 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     4561 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     4621 aaacaaagtt gggcgaaccc ggagcctcat taattgttag ccgttaaaat taagcccttt
     4681 accaaaccaa tacttattat gaaaaacaca atacatatca acttcgctat ttttttaata
     4741 attgcaaata ttatctacag cagcgccagt gcatcaacag atatctctac tgttgcatct
     4801 ccattatttg aaggaactga aggttgtttt ttactttacg atgcatccac aaacgctgaa
     4861 attgctcaat tcaataaagc aaagtgtgca acgcaaatgg caccagattc aactttcaag
     4921 atcgcattat cacttatggc atttgatgcg gaaataatag atcagaaaac catattcaaa
     4981 tgggataaaa cccccaaagg aatggagatc tggaacagca atcatacacc aaagacgtgg
     5041 atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc aaaaaattgg attaaataaa
     5101 atcaagaatt atctcaaaga ttttgattat ggaaatcaag acttctctgg agataaagaa
     5161 agaaacaacg gattaacaga agcatggctc gaaagtagct taaaaatttc accagaagaa
     5221 caaattcaat tcctgcgtaa aattattaat cacaatctcc cagttaaaaa ctcagccata
     5281 gaaaacacca tagagaacat gtatctacaa gatctggata atagtacaaa actgtatggg
     5341 aaaactggtg caggattcac agcaaataga accttacaaa acggatggtt tgaagggttt
     5401 attataagca aatcaggaca taaatatgtt tttgtgtccg cacttacagg aaacttgggg
     5461 tcgaatttaa catcaagcat aaaagccaag aaaaatgcga tcaccattct aaacacacta
     5521 aatttataaa aaatctaatg gcaaaatcgc ccaacccttc aatcaagtcg ggacggccaa
     5581 aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc ttatttcaaa cgttaaacat
     5641 catgagggaa gtggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     5701 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     5761 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     5821 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     5881 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     5941 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     6001 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     6061 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     6121 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     6181 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     6241 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     6301 tcagcccgtc atacttgaag ctaggcaggc ttatcttgga caagaagatc gcttggcctc
     6361 gcgcgcagat cagttggaag aatttgttca ctacgtgaaa ggcgagatca ccaaggtagt
     6421 cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
     6481 aagcgttaga gagctgggga agactatgcg cgatctgttg aaggtggttc taagcctcgt
     6541 acttgcgatg gcatcggggc aggcacttgc tgacctgcca attgttttag tggatgaagc
     6601 tcgtcttccc tatgactact ccccatccaa ctacgacatt tctccaagca actacgacaa
     6661 ctccataagc aattacgaca atagtccatc aaattacgac aactctgaga gcaactacga
     6721 taatagttca tccaattacg acaatagtcg caacggaaat cgtaggctta tatatagcgc
     6781 aaatgggtct cgcactttcg ccggctacta cgtcattgcc aacaatggga caacgaactt
     6841 cttttccaca tctggcaaaa ggatgttcta caccccaaaa ggggggcgcg gcgtctatgg
     6901 cggcaaagat gggagcttct gcggggcatt ggtcgtcata aatggccaat tttcgcttgc
     6961 cctgacagat aacggcctga agatcatgta tctaagcaac tagcctgctc tctaataaaa
     7021 tgttaggcct caacatctag tcgcaagctg aggggaacca ctagtgtcat acgaacctcc
     7081 aagagacggt tacacaaacg ggtacattgt tgatgtcatg tatgacaatc gcccaagtaa
     7141 gtatccagct gtgttcagaa cgtacgtccg aattccagac tatgtcgaac tagataacgc
     7201 ggaggagctg gccgacgcaa tcattcgaga ggttgagtta aaggaggcat ggctcacaag
     7261 ggtcttgggg aactcagccg atcctgacgc ttctgtgggc aagtgggacc ggcgcctagc
     7321 gcagtgcggt ggtattgatg agatgtcaag tggggtgcag gcaacatact tcagtccaaa
     7381 cggtcagctg gtcggcttag tggcatacat tgatgccgaa tggatggatg aatagctctg
     7441 acgttacgat tcaggtgaga caaccagatg ggcattgcgt acaagctcag ttgccaccga
     7501 tgtggctacg agtctgatct tctttaccta ggccaaggta tggcgatgct tcctgagcaa
     7561 atagtcggaa cgtgcacatg ctgcgacaat ctaacgacga tttctataaa tgaaagctac
     7621 ggagtatgct cactttgtgg ggctaaggat cgtgtgatat ttacaagcta tcagcatgag
     7681 gcaaggccaa gaaatccctg ctatctagag agaagtttca aatatcaatg cccaagatgc
     7741 catgccttca gtatggagcc tccggatcta ccggagatgc tttgggacta atgctccgct
     7801 ttgcctcggg tatccgttcg acctatgttt aagaggaaac gcacgtgtct gccgccaaag
     7861 ctcttgccta tgcactttgt gacaagttgg aaagcaaaaa agttgatgaa gtcgtacga
//
LOCUS       DAJTNN010000469         6058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.guided.210, whole genome shotgun sequence.
ACCESSION   DAJTNN010000469 DAJTNN010000000
VERSION     DAJTNN010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.guided.210"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            980..1795
                     /gene="sul2"
                     /locus_tag="OO150_004424"
     CDS             980..1795
                     /gene="sul2"
                     /locus_tag="OO150_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5518311.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1882..2184
                     /locus_tag="OO150_004425"
     CDS             1882..2184
                     /locus_tag="OO150_004425"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518312.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2360..3853)
                     /locus_tag="OO150_004426"
     CDS             complement(2360..3853)
                     /locus_tag="OO150_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR5518313.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(3897..4286)
                     /locus_tag="OO150_004427"
     CDS             complement(3897..4286)
                     /locus_tag="OO150_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518314.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4504..4944
                     /locus_tag="OO150_004428"
     CDS             4504..4944
                     /locus_tag="OO150_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5518315.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5678..5827)
                     /locus_tag="OO150_004429"
     CDS             complement(5678..5827)
                     /locus_tag="OO150_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518316.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1434 a         1670 c         1589 g         1365 t
ORIGIN      
        1 tgactcgcta cgctcggtcg ttcggctgcg gcgagcggta ccggcttacg gacggggacg
       61 gaaatttcct ggaagatacc aggaagacac ttagcaaaga agagataagg ccacggcgaa
      121 gccgtttttc cataggctcc gcccccctga caagcatcgc aaaatctgac gctcaaatca
      181 gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct
      241 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      301 gtttctcatt ccacgcctga cactcagttc cgtgaaggca gttcgctcca agctggactg
      361 tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg tccggtaact atcaacttga
      421 gtccaacccg gaaagacacg acaaaacgcc actggcagca gccattagta acaggagtgc
      481 acgagaagat agattaagag gtcttgaagt agaggcctga ctgcggctac actgaaagaa
      541 cagttttggt aatggcggtc tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga
      601 cttaaccttc gaacaaaccg cctccccagg cggttttttc gtttacagag caagagatta
      661 cgattcattg aaaaggatct caagaagatc ctttgtagat tactatcaat taatcttccg
      721 aacatgacac cagtcactgt tgattacggt taaaccgctc cgtaacaggt ttccctcgta
      781 ttggtgactt gcagcaccaa agaagcaaaa aagataaata tatctactta ttgagagatg
      841 atatatttat ttccaaaatg ctacagatac atcatcatga aacggatcac cgcaaacagg
      901 ttactcgcct ggggattccc tttcgacccg agcatccgta tgagactcat gctcgattat
      961 tattattata gaagccccca tgaataaatc gctcatcatt ttcggcatcg tcaacataac
     1021 ctcggacagt ttctccgatg gaggccggta tctggcgcca gacgcagcca ttgcgcaggc
     1081 gcgtaagctg atggccgagg gggcagatgt gatcgacctc ggtccggcat ccagcaatcc
     1141 cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc
     1201 gctcaaggca gatggcattc ccgtctcgct cgacagttat caacccgcga cgcaagccta
     1261 tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc ggttttccag acgctgcgtt
     1321 ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt atgcattcgg tgcaagacgg
     1381 gcaggcagat cggcgcgagg cacccgctgg cgacatcatg gatcacattg cggcgttctt
     1441 tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga
     1501 tcccggcatg ggtttttttc tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg
     1561 gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc
     1621 ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc ggggccgcga cactcgctgc
     1681 agagcttgcc gccgccgcag gtggagctga cttcatccgc acacacgagc cgcgcccctt
     1741 gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc gcaagaattc gttaactgca
     1801 cattcgggat atttctctat attcgcggtt cagcaggcat gtcccctttg agggcgaccc
     1861 gacgacagga taatcgacct tatggtgcgc aaatatttcg gcacagacgg tattcgtggc
     1921 aaagccaacg aaggcgcgat gacggcggaa accgccttgc gcgtcggcat ggcggctggc
     1981 cgtgtctttc gtcgcggtga ccaccgccat cgtgtcgtga tcggcaagga tacgcgcctg
     2041 tcgggctata tgcttgaacc cgcgctcaca gccggtttca cctcgatggg catggacgta
     2101 ttcctttttg gcccgctgcc gacaacgtat aggaagaata aacgcccttt tcacccaagt
     2161 ccaacagctt tggaccgcag ttgactcttt cgacacccct gcgatgcaac ccaatccggc
     2221 tgacggggag ccagcaacgc tgaaaattta ccctcctctt tcccactagc ggctcctttt
     2281 ccgacaacca gcacggcgga tccctgccgc ggcgctgtga acgcagcatt ttgattggta
     2341 tcgttggcct tcaggctcgt cagtcaaaca gacccaggag cagctcagcc ggtggcgccc
     2401 ggctttcggg taacgccctg gtcccgctgg tttcggcttt gtgcttcagg tgatcaagga
     2461 tctgcttgat cactataggg tcttcaatgc aggcgatgac tttcatggcg ccgccgcagc
     2521 cgctgcaggt ctcgatgtcg atattgaaaa cacgcttgag ccgttgcgcc catgtcatcg
     2581 acgctcgccg ttgtgctggt gttgccggtt catcagccac cctgaccttg ttgcccctgc
     2641 cccgttttgc cggcgtgacc aacgcccggt gccgactgtt gggtgcgaac accccgtgga
     2701 agcgggttag gttgactctg ggcttcggta ccagggcggc cagccttgca atgaaatcca
     2761 atggttcgaa aatgacgtgc gtggtgccgt cccggtacgg cgtcttgagc tggtagcgca
     2821 cgttgccgcc tcgtgttaac gacagccgct tctcggatac cgccgggcgg ctgatgtacc
     2881 ggcacagccg ttcgagcttc ttgcgttcat cggccctggc cgccacgccg gcgtgcaggc
     2941 tggacccggc taccttgcca atcccgtcac cgaacggatc accactggtc ggcagagttt
     3001 gcaaagtgaa cacctttcgc cccgcctgtg aaccgacagc gatacggtaa gtgatcgagt
     3061 gccccagcag gggtgtcatc gggtcgtcat ccaccgcatc cgaggccaga tagctgtttt
     3121 cgacatcccg ttccagcagg ccttgccgtt ccagatagcg acccacccgg tgggcgatgg
     3181 tgtgcgtcag ctgggtgagc tctgggctgg tcggcgcctt gacccagcgg aaacgcgctg
     3241 agccgtggga ttgctcgaca tacacaccgt cgagaaacag catgtggaag tgaacattca
     3301 gattgagcgc cgatccaaaa cgctggatca gggtgaccgc gcccgtcttg gccacttggt
     3361 gggtatggcc cgctttcttg accaggtgcg tggcaatgac gcggtaaacg atgcccagca
     3421 cccaccccat gatctcgggc cggctggcaa acaggaaacg cagctgaaac gggaagctca
     3481 acacccactg acgcatgggt tgttcaggca gtacttcatc aaccagcaag gcggcacttt
     3541 cggccatccg ccgcgcccca cagctcgggc agaaaccgcg acgcttacag ctgaaagcga
     3601 ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc tccagccgcc
     3661 cgcattggag aaattcttca aattcccgtt gcacatagcc cggcaattcc tttccctgct
     3721 ctgccataag cgcagcgaat gccgggtaat actcgtcaac gatctgatag agaagggttt
     3781 gctcgggtcg gtggctctgg taacgaccag tatcccgatc ccggctggcc gtcctggccg
     3841 ccacatgagg catgttccgc gtccttgcaa tactgtgttt acatacagtc tatcgcttag
     3901 cggaaagttc ttttaccctc agccgaaatg cctgccgttg ctagacattg ccagccagtg
     3961 cccgtcactc ccactcttac attgttcgca gtatctaggt acatgttatt gcgtgcacct
     4021 tcatgatgta taatgttatt aatgacagca gaaagatcga cagaattaat attcttcgta
     4081 cctgggacag ttatcgggtg tcggccagtg atgagaccaa aggatgcagt ataagatgaa
     4141 ccactactat tatcatggcc accattacca ccaccgttaa aactacctcc cgaacttcca
     4201 gagccgctat gaccatcatt gtaggggtca tttggtccca tgtgaccaaa accaccgaca
     4261 gtaccgctat ttaactctgt actcataaaa ttatcctctt tgacttttaa aaacagcaaa
     4321 aactgtattt ataacaatgt gtttatatac agtattcgaa atactacgcc cgtcaaaacc
     4381 acaccgtcaa caggtttttt gtgtattttt cacgcctcac gggcagcctc agaccttttg
     4441 tgttaaaaca gtaatatgtt ctgctgttac aatacggtac acacaggaga cactgtcact
     4501 tttatggcat ggcacacagt tcaagagaca ctgaaattaa caggaaagtc gcgctcgcaa
     4561 ttatatcgtg acatggctaa aggccttgtc agctatcgca caggaaaaaa tgatcgccgg
     4621 gagttcgaga ccagtgaact gattcgggcc tatggcgagc tgcgcacttc tgagacacca
     4681 aaaaggcaca aggagggaca ctccaaatct gtcgttagcc aggcggaaaa tgctcagcta
     4741 gacgtcattc agcagaaact ggacgctctg cagcagactg ttaccctaat gcttgaagac
     4801 aagatggcgc gggaagcaga aaaccgcctg cgaaatgagg agagggaaca actacaggcc
     4861 gaaattagcc gtctggctga cgcgctggca caggaaaaaa agcggggatt ctggtcgcgg
     4921 ctgtttcgct ccggttcaga gtaacagtgc aggtgactct gatttaccat aacatctgtt
     4981 atcagcaccg cctgagaggc cctcagggcg tttctgacgg gaaaccggta aaacaggcaa
     5041 caaaccaccc gaaaacccgc cagaaacgcg caggctctgt ttttacggca tgcatgagta
     5101 tgcgcacaag tgcatagcgc ggaaattcac accattcagc cggggtaagt cctgtttccg
     5161 gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg
     5221 ttgccggcgc agccggaaaa cccgctgtca gcacgttcag gaagggggtg atgtacaaat
     5281 ggcacagagg atgaaagtga cgtatgagct gaaggaatga tgtaaaagtg atgtataagt
     5341 gatgtatgaa ctgaaggagc gatgtaattc tgtagtcagg aagaataacc cgttcatgca
     5401 ggcatgaacg atacagtgag gaacgctttt tttgtcacga tctcccctta acctgcgtaa
     5461 atcacccgcc atggttgatt atttttcaac ctcaaaatgg tcatacacct aacgcagccg
     5521 cgattttcat cgcaactgca tgaattaaaa gaaaaaaaag gaatatttcg ttttcacgag
     5581 taatacccgg gtattaaaaa tccactgtaa ttacatgaaa ataaagaaat aaaaggtaat
     5641 accggagtat taccacgtac gcgggcaagc gaaaaagcta taacgcttcc cggccgacag
     5701 gcgacccaaa atccacaggg gcatgcaggt tctcttcagt aacctgcgcc attaaatcaa
     5761 aaaggtcata ttcgccggga agctgcgtat cagtcgaatc cagttgttca tcatattcag
     5821 atggcatggg gtgatatcca gtagctgaga ggttgttagc gggtttcggg gcgcagccct
     5881 gaaccagtca tgtagcgcta gcggagtgta tactggctta ctatgttgcc acgattgcga
     5941 aaatcagtga agtgcttcat gtggcaggag aaaaaaggca gcaccggcgc gtcagcagaa
     6001 tatgcgatac aggagatatt ccgcttcctc gctcactgac tcgctacgct cggtcgtt
//
LOCUS       DAJTNN010000470         5666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.guided.220, whole genome shotgun sequence.
ACCESSION   DAJTNN010000470 DAJTNN010000000
VERSION     DAJTNN010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.guided.220"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            70..285
                     /locus_tag="OO150_004430"
     CDS             70..285
                     /locus_tag="OO150_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5518317.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            278..664
                     /locus_tag="OO150_004431"
     CDS             278..664
                     /locus_tag="OO150_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5518318.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            672..1358
                     /locus_tag="OO150_004432"
     CDS             672..1358
                     /locus_tag="OO150_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5518319.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="OO150_004433"
     CDS             complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="OO150_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5518320.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2041..3246
                     /gene="tet(B)"
                     /locus_tag="OO150_004434"
                     /pseudo
     CDS             2041..3246
                     /gene="tet(B)"
                     /locus_tag="OO150_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
     gene            complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="OO150_004435"
     CDS             complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="OO150_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5518321.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4085..4456
                     /locus_tag="OO150_004436"
     CDS             4085..4456
                     /locus_tag="OO150_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5518322.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELKLTKKTILEIALQYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4466..>5666)
                     /locus_tag="OO150_004437"
     CDS             complement(4466..>5666)
                     /locus_tag="OO150_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5518323.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1570 a         1046 c         1229 g         1821 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta
       61 gctggtttta tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg
      121 ctatcttggt gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg
      181 aaagctcaac aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat
      241 attactcgcg aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt
      301 agataatcaa ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg
      361 attggaaggg ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga
      421 acaaggaaag gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg
      481 gacaccatta attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg
      541 agtattggcg gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact
      601 gatattaata acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa
      661 gtgaacttgc gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg
      721 cgataggggc ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg
      781 ggcgagcttg gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga
      841 gcagtcattt atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc
      901 atcgttattt tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga
      961 tctccttaaa ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac
     1021 gtacttgcta tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc
     1081 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
     1141 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
     1201 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
     1261 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
     1321 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     1381 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     1441 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     1501 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     1561 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     1621 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     1681 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     1741 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     1801 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     1861 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     1921 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
     1981 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     2041 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     2101 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     2161 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     2221 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     2281 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     2341 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     2401 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     2461 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     2521 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     2581 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     2641 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     2701 caatagatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     2761 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     2821 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     2881 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     2941 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     3001 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     3061 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     3121 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     3181 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     3241 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     3301 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     3361 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     3421 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     3481 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     3541 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     3601 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     3661 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     3721 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     3781 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct
     3841 catctattga aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca
     3901 gtgattgata ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt
     3961 taaaataaca taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta
     4021 agaacattta ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa
     4081 taacatccaa ttaagatatg atgataagca gtttcataca acggttatca aagatgttct
     4141 attatggatt gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc
     4201 gggttatacc aagtggtatt ttcagcggct gttcaaaaaa gtaacagggg tcacactggc
     4261 tagctatatt cgtgctcgtc gtttgacgaa agcggctgtt gagttgaagt tgacgaaaaa
     4321 aactatcctt gagatcgcat tacaatatca atttgattcc caacaatctt ttacacgtcg
     4381 atttaagtac atttttaagg ttacaccaag ttattatcgg cgtaataaat tatgggaatt
     4441 ggaggcaatg cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata
     4501 aattttgagc tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag
     4561 aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg
     4621 tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac
     4681 atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg
     4741 ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt
     4801 caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag
     4861 gtaagttagt tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg
     4921 ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat
     4981 acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct
     5041 ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta
     5101 ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa
     5161 cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac
     5221 tcggtagaat gctcgcaagg tcggctagaa attggtcatg agctttcttt gaacattgct
     5281 ctgaaagcgg gaacgctttc tcataaagag taacagaacg accgtgtagt gcgactgaag
     5341 ctcgcaatac cataagtcgt ttttgctcac gaatatcaga ccagtcaaca agtacaatgg
     5401 gcatcgtatt gcccgaacag ataaagctag catgccaacg gtatacagcg agtcgctctt
     5461 tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttatgt tttgttctcg
     5521 ctttggttgg caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaatg
     5581 cgtggcaagc caacgttaag ctgttgagtc gttttaagtg taattcgggg taatgactcc
     5641 aacttactga tagtgtttta tgttca
//
LOCUS       DAJTNN010000471         5666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.guided.221, whole genome shotgun sequence.
ACCESSION   DAJTNN010000471 DAJTNN010000000
VERSION     DAJTNN010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.guided.221"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            70..285
                     /locus_tag="OO150_004438"
     CDS             70..285
                     /locus_tag="OO150_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5518324.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            278..664
                     /locus_tag="OO150_004439"
     CDS             278..664
                     /locus_tag="OO150_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5518325.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            672..1358
                     /locus_tag="OO150_004440"
     CDS             672..1358
                     /locus_tag="OO150_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5518326.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="OO150_004441"
     CDS             complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="OO150_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5518327.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2041..3246
                     /gene="tet(B)"
                     /locus_tag="OO150_004442"
     CDS             2041..3246
                     /gene="tet(B)"
                     /locus_tag="OO150_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5518328.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="OO150_004443"
     CDS             complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="OO150_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5518329.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4085..4456
                     /locus_tag="OO150_004444"
     CDS             4085..4456
                     /locus_tag="OO150_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5518330.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELKLTKKTILEIALQYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4466..>5666)
                     /locus_tag="OO150_004445"
     CDS             complement(4466..>5666)
                     /locus_tag="OO150_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5518331.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1569 a         1046 c         1229 g         1822 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta
       61 gctggtttta tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg
      121 ctatcttggt gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg
      181 aaagctcaac aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat
      241 attactcgcg aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt
      301 agataatcaa ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg
      361 attggaaggg ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga
      421 acaaggaaag gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg
      481 gacaccatta attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg
      541 agtattggcg gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact
      601 gatattaata acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa
      661 gtgaacttgc gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg
      721 cgataggggc ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg
      781 ggcgagcttg gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga
      841 gcagtcattt atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc
      901 atcgttattt tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga
      961 tctccttaaa ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac
     1021 gtacttgcta tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc
     1081 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
     1141 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
     1201 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
     1261 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
     1321 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     1381 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     1441 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     1501 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     1561 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     1621 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     1681 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     1741 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     1801 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     1861 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     1921 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
     1981 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     2041 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     2101 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     2161 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     2221 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     2281 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     2341 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     2401 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     2461 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     2521 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     2581 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     2641 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     2701 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     2761 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     2821 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     2881 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     2941 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     3001 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     3061 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     3121 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     3181 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     3241 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     3301 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     3361 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     3421 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     3481 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     3541 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     3601 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     3661 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     3721 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     3781 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct
     3841 catctattga aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca
     3901 gtgattgata ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt
     3961 taaaataaca taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta
     4021 agaacattta ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa
     4081 taacatccaa ttaagatatg atgataagca gtttcataca acggttatca aagatgttct
     4141 attatggatt gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc
     4201 gggttatacc aagtggtatt ttcagcggct gttcaaaaaa gtaacagggg tcacactggc
     4261 tagctatatt cgtgctcgtc gtttgacgaa agcggctgtt gagttgaagt tgacgaaaaa
     4321 aactatcctt gagatcgcat tacaatatca atttgattcc caacaatctt ttacacgtcg
     4381 atttaagtac atttttaagg ttacaccaag ttattatcgg cgtaataaat tatgggaatt
     4441 ggaggcaatg cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata
     4501 aattttgagc tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag
     4561 aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg
     4621 tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac
     4681 atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg
     4741 ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt
     4801 caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag
     4861 gtaagttagt tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg
     4921 ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat
     4981 acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct
     5041 ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta
     5101 ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa
     5161 cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac
     5221 tcggtagaat gctcgcaagg tcggctagaa attggtcatg agctttcttt gaacattgct
     5281 ctgaaagcgg gaacgctttc tcataaagag taacagaacg accgtgtagt gcgactgaag
     5341 ctcgcaatac cataagtcgt ttttgctcac gaatatcaga ccagtcaaca agtacaatgg
     5401 gcatcgtatt gcccgaacag ataaagctag catgccaacg gtatacagcg agtcgctctt
     5461 tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttatgt tttgttctcg
     5521 ctttggttgg caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaatg
     5581 cgtggcaagc caacgttaag ctgttgagtc gttttaagtg taattcgggg taatgactcc
     5641 aacttactga tagtgtttta tgttca
//
LOCUS       DAJTNN010000472         3330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.guided.270, whole genome shotgun sequence.
ACCESSION   DAJTNN010000472 DAJTNN010000000
VERSION     DAJTNN010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.guided.270"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            <1..571
                     /locus_tag="OO150_004446"
     CDS             <1..571
                     /locus_tag="OO150_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HCR5518332.1"
                     /translation="RAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMG
                     LAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIELGHGLNV
                     AECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITT
                     LPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            564..1298
                     /gene="frdB"
                     /locus_tag="OO150_004447"
     CDS             564..1298
                     /gene="frdB"
                     /locus_tag="OO150_004447"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5518333.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1309..1704
                     /gene="frdC"
                     /locus_tag="OO150_004448"
     CDS             1309..1704
                     /gene="frdC"
                     /locus_tag="OO150_004448"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5518334.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1715..2074
                     /gene="frdD"
                     /locus_tag="OO150_004449"
     CDS             1715..2074
                     /gene="frdD"
                     /locus_tag="OO150_004449"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5518335.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            2137..3270
                     /locus_tag="OO150_004450"
     CDS             2137..3270
                     /locus_tag="OO150_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5518336.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT          826 a          866 c          886 g          752 t
ORIGIN      
        1 gcgtgcagca actgccggta atggcaacga agaggcaatt gaagcgcagg cagctggcgt
       61 tgaacaacgt ctgaaagatc tggttaacca ggatggcggc gaaaactggg cgaagatccg
      121 cgacgaaatg ggcctagcaa tggaagaagg ttgcggtatc taccgtacgc cggaactgat
      181 gcagaaaacc atcgacaagc tggcagagct gcaggaacgc ttcaagcgcg tgcgcatcac
      241 cgacacctcc agcgtgttca acaccgacct gctctacacc attgaactgg gccacggtct
      301 gaacgttgct gaatgtatgg cgcactccgc aatggcacgt aaagagtccc gcggcgcaca
      361 ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc aacttcctca aacacaccct
      421 cgccttccgc gatgctgatg gcacgactcg cctggagtac agcgacgtga agattactac
      481 gctgccgcca gctaaacgcg tttacggtgg cgaagcggat gcagccgata aggcggaagc
      541 agccaataag aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt gaggtggtgc
      601 gctataaccc ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg ccttatgacg
      661 caactacctc attactggat gcgctgggct acatcaaaga caacctggca ccggacctga
      721 gttaccgctg gtcctgccgt atggcgattt gtggttcctg cggcatgatg gttaacaacg
      781 tgccaaaact ggcatgtaaa accttcctgc gtgattacac cgacggtatg aaggttgaag
      841 cgttagctaa cttcccgatt gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa
      901 gtctggaagc gatcaaaccg tacatcatcg gcaactcccg caccgcggat cagggtacta
      961 acatccagac cccggcgcag atggcaaagt atcaccagtt ctccggttgc atcaactgtg
     1021 gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc ggtccggctg
     1081 ccattacgct ggcgcatcgt tataacgaag atagccgcga ccacggtaag aaggagcgta
     1141 tggcgcagtt gaacagccag aacggcgtat ggagctgtac tttcgtgggc tattgctccg
     1201 aagtctgccc gaaacacgtc gatccggctg cggccattca gcagggcaaa gtagaaagtt
     1261 cgaaagactt tcttatcgcg accctgaaac cacgctaagg agtgcaacat gacgactaaa
     1321 cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga aaaaattgcc gttttatcgc
     1381 ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat tgaactgatt
     1441 ttcgggctgt ttgccctgaa aaatggcccg gacgcctggg cgggattcgt cgacttttta
     1501 caaaacccgg ttatcgtgat cattaacctg atcactctgg cggcagctct gctgcacacc
     1561 aaaacctggt ttgaactggc accgaaagcg gccaatatca ttgtaaaaga cgaaaaaatg
     1621 ggaccagagc caattatcaa aagtctctgg gcggtaactg tggttgccac catcgtaatc
     1681 ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt aatccaaatc caaagcgttc
     1741 tgacgaaccg gtattctggg gcctcttcgg ggccggtggt atgtggagcg caattattgc
     1801 gccggtgatg atcctgctgg tgggtattct gctgccactg gggctgtttc cgggtgatgc
     1861 gctgagctac gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg tattcctgtt
     1921 cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg cgatgcacga
     1981 tctgaaaatt cacgtgcctg cgggcaaatg ggttttctac ggtctggctg ctatcctgac
     2041 agttgtcacg ctgattggta tcgttacaat ctaacgtatc gccaatgtaa atccggcccg
     2101 cctatggcgg gccgttttgt atggaaacca gaccttatgt tcaaaacgac gctctgcgcc
     2161 ttattaatta ccgcctcttg ctccacattt gctgcccctc aacaaatcaa cgatattgtg
     2221 catcgcacaa ttaccccgct tatagagcaa caaaagatcc ccggtatggc ggtggcggta
     2281 atttatcagg gtaaacctta ttactttacc tggggctatg cggacatcgc caaaaagcag
     2341 cccgtcacac agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt tacgggcgtg
     2401 cttggtggcg atgctattgc tcgaggggaa atcaagttaa gcgatcccgc aacaaaatac
     2461 tggcctgaac ttaccgctaa acagtggaat gggatcacac tattacatct tgcgacctac
     2521 accgctggcg gcctgccatt gcagatgccg gatgaagtga aatcctcaag cgacttgctg
     2581 cgcttctatc aaaactggca gcctgcatgg gctccaggaa cacaacgtct gtatgccaac
     2641 tccagtatcg gtttgttcgg cgcactggct gtgaagccgt ctggtttgag ttttgagcag
     2701 gcgatgcaaa ctcgtgtctt ccagccactc aaactcaacc atacgtggat taatgtaccg
     2761 cccgcagaag aaaagaatta cgcctgggga tatcgcgaag gcaaggcagt tcatgtttcg
     2821 ccaggggcgt tagatgctga agcttatggt gtgaagtcga ccattgaaga tatggcccgc
     2881 tgggtgcaaa gcaatttaac cccccttgat atcaatgaga aaacgcttca acaagggata
     2941 caactggcac aatctcgcta ctggcaaacc ggcgatatgt atcagggcct gggctgggaa
     3001 atgctggact ggccggtcaa tcctgacagc atcattaacg gcagtgacaa taaaattgca
     3061 ctggcagcac gcccggtaaa agcgattaag cccccaactc ctgcagtacg cgcatcatgg
     3121 gtacataaaa cgggggcgac cggcggattt ggtagctatg tcgcgtttat tccagaaaaa
     3181 gagctgggta tcgtgatgct ggctaacaaa aactatccca atccagcgag agtcgccgcc
     3241 gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt ttcggacctt
     3301 ttctgcgctt ttccttgctg tcatctacac
//
LOCUS       DAJTNN010000473         2586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.guided.291, whole genome shotgun sequence.
ACCESSION   DAJTNN010000473 DAJTNN010000000
VERSION     DAJTNN010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.guided.291"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..770)
                     /gene="pepD"
                     /locus_tag="OO150_004451"
     CDS             complement(<1..770)
                     /gene="pepD"
                     /locus_tag="OO150_004451"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HCR5518337.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNA"
     gene            1031..1489
                     /gene="gpt"
                     /locus_tag="OO150_004452"
     CDS             1031..1489
                     /gene="gpt"
                     /locus_tag="OO150_004452"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5518338.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1581..>2586
                     /gene="frsA"
                     /locus_tag="OO150_004453"
     CDS             1581..>2586
                     /gene="frsA"
                     /locus_tag="OO150_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5518339.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     L"
BASE COUNT          600 a          665 c          674 g          647 t
ORIGIN      
        1 gcgttacgca gtgtgccgcc gttgaaatcg ataaggcgca ggtccagttc ttctgcatga
       61 cccgccagga agcgcaccag cagtttgttg gcattaccca ggccaacgtg gatttcaccg
      121 ccggagtgac cgcctttcag accttttaag gttaacttga aggtttcaaa accagctgga
      181 accgcttcac gatctaaatg caggttggag gtgaagtcga taccccccgc acaacccatg
      241 tagatttcac cttcttcttc ggagtcggtg ttaatcagaa tatcagcctg caaccagttg
      301 ctctgtaagc caaaagcacc gtccataccg gcttcttcgg tcatggtcag cagcacttcc
      361 agcggaccgt gaaccacgtt ttcgtcagcc agaaccgcca gtgcagaggc cataccaatg
      421 ccgttatccg cacccagcgt ggtgccgcgc gctttaaccc attcgccatc aatataaggc
      481 tggataggat ctttcgtgaa gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg
      541 aggtgggcct gtaagacgac cggtttacga ttttccatac ctgcggtagc aggtttacga
      601 atcaggatat tacctacctg atcgcgttcg acatggaaac ctttctcttt tgcccaacca
      661 acaatgtatt cagcgagttg ctcttcatga taggacgggt gcggaataga acagattttg
      721 gcaaaaatat cccacagcgg ctgtggagat aattgagaca gttcagacac gttaagtctc
      781 cttgtcgatc acccgcaaaa cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa
      841 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
      901 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
      961 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     1021 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     1081 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     1141 tggcggtctg gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     1201 cgtttgtatt tccagctacg atcacgacaa ccagcgagag cttaaagtgc tgaaacgcgc
     1261 agaaggcgat ggcgaaggct tcatagttat tgatgacctg gtggataccg gtggtactgc
     1321 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     1381 tggtcgtccg ctggttgatg actatgttgt tgatattccg caagatacct ggatcgaaca
     1441 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     1501 cctggcactg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
     1561 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     1621 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     1681 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     1741 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
     1801 tgatgagcga tgctgaacgt accgacgatg atttatacga tactgtgatt ggctaccgtg
     1861 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgtg
     1921 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
     1981 ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa
     2041 accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta
     2101 ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt
     2161 tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt
     2221 atgaacgtta ttttgcgccg cgcggcattg tgatgctgac tattgatatg ccgtcggtgg
     2281 gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa
     2341 aggcgctgcc taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt
     2401 tcggcgctaa cgttgccgtg cgtctggcat atcttgaatc gccgcgtctg aaagcggttg
     2461 cctgtcttgg tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc
     2521 cggaaatgta tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag
     2581 cgttac
//
LOCUS       DAJTNN010000474         2290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.guided.305, whole genome shotgun sequence.
ACCESSION   DAJTNN010000474 DAJTNN010000000
VERSION     DAJTNN010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.guided.305"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            10..801
                     /gene="aadA1"
                     /locus_tag="OO150_004454"
     CDS             10..801
                     /gene="aadA1"
                     /locus_tag="OO150_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5518340.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            877..1374
                     /locus_tag="OO150_004455"
     CDS             877..1374
                     /locus_tag="OO150_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518341.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1435..1806
                     /locus_tag="OO150_004456"
     CDS             1435..1806
                     /locus_tag="OO150_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518342.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
BASE COUNT          598 a          548 c          605 g          539 t
ORIGIN      
        1 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
       61 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
      121 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
      181 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
      241 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
      301 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
      361 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
      421 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
      481 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
      541 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
      601 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
      661 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
      721 cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa aggcgagatc
      781 accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg
      841 cggcttaact caagcgttag agagctgggg aagactatgc gcgatctgtt gaaggtggtt
      901 ctaagcctcg tacttgcgat ggcatcgggg caggcacttg ctgacctgcc aattgtttta
      961 gtggatgaag ctcgtcttcc ctatgactac tccccatcca actacgacat ttctccaagc
     1021 aactacgaca actccataag caattacgac aatagtccat caaattacga caactctgag
     1081 agcaactacg ataatagttc atccaattac gacaatagtc gcaacggaaa tcgtaggctt
     1141 atatatagcg caaatgggtc tcgcactttc gccggctact acgtcattgc caacaatggg
     1201 acaacgaact tcttttccac atctggcaaa aggatgttct acaccccaaa aggggggcgc
     1261 ggcgtctatg gcggcaaaga tgggagcttc tgcggggcat tggtcgtcat aaatggccaa
     1321 ttttcgcttg ccctgacaga taacggcctg aagatcatgt atctaagcaa ctagcctgct
     1381 ctctaataaa atgttaggcc tcaacatcta gtcgcaagct gaggggaacc actagtgtca
     1441 tacgaacctc caagagacgg ttacacaaac gggtacattg ttgatgtcat gtatgacaat
     1501 cgcccaagta agtatccagc tgtgttcaga acgtacgtcc gaattccaga ctatgtcgaa
     1561 ctagataacg cggaggagct ggccgacgca atcattcgag aggttgagtt aaaggaggca
     1621 tggctcacaa gggtcttggg gaactcagcc gatcctgacg cttctgtggg caagtgggac
     1681 cggcgcctag cgcagtgcgg tggtattgat gagatgtcaa gtggggtgca ggcaacatac
     1741 ttcagtccaa acggtcagct ggtcggctta gtggcataca ttgatgccga atggatggat
     1801 gaatagctct gacgttacga ttcaggtgag acaaccagat gggcattgcg tacaagctca
     1861 gttgccaccg atgtggctac gagtctgatc ttctttacct aggccaaggt atggcgatgc
     1921 ttcctgagca aatagtcgga acgtgcacat gctgcgacaa tctaacgacg atttctataa
     1981 atgaaagcta cggagtatgc tcactttgtg gggctaagga tcgtgtgata tttacaagct
     2041 atcagcatga ggcaaggcca agaaatccct gctatctaga gagaagtttc aaatatcaat
     2101 gcccaagatg ccatgccttc agtatggagc ctccggatct accggagatg ctttgggact
     2161 aatgctccgc tttgcctcgg gtatccgttc gacctatgtt taagaggaaa cgcacgtgtc
     2221 tgccgccaaa gctcttgcct atgcactttg tgacaagttg gaaagcaaaa aagttgatga
     2281 agtcgtacga
//
LOCUS       DAJTNN010000475         2287 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.guided.306, whole genome shotgun sequence.
ACCESSION   DAJTNN010000475 DAJTNN010000000
VERSION     DAJTNN010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.guided.306"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            10..798
                     /gene="aadA1"
                     /locus_tag="OO150_004457"
     CDS             10..798
                     /gene="aadA1"
                     /locus_tag="OO150_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5518343.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            874..1371
                     /locus_tag="OO150_004458"
     CDS             874..1371
                     /locus_tag="OO150_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518344.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1432..1803
                     /locus_tag="OO150_004459"
     CDS             1432..1803
                     /locus_tag="OO150_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5518345.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
BASE COUNT          595 a          548 c          605 g          539 t
ORIGIN      
        1 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
       61 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
      121 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
      181 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
      241 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
      301 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
      361 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
      421 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
      481 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
      541 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
      601 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
      661 gcccagtatc agcccgtcat acttgaagct aggcaggctt atcttggaca agaagatcgc
      721 ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc
      781 aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg
      841 cttaactcaa gcgttagaga gctggggaag actatgcgcg atctgttgaa ggtggttcta
      901 agcctcgtac ttgcgatggc atcggggcag gcacttgctg acctgccaat tgttttagtg
      961 gatgaagctc gtcttcccta tgactactcc ccatccaact acgacatttc tccaagcaac
     1021 tacgacaact ccataagcaa ttacgacaat agtccatcaa attacgacaa ctctgagagc
     1081 aactacgata atagttcatc caattacgac aatagtcgca acggaaatcg taggcttata
     1141 tatagcgcaa atgggtctcg cactttcgcc ggctactacg tcattgccaa caatgggaca
     1201 acgaacttct tttccacatc tggcaaaagg atgttctaca ccccaaaagg ggggcgcggc
     1261 gtctatggcg gcaaagatgg gagcttctgc ggggcattgg tcgtcataaa tggccaattt
     1321 tcgcttgccc tgacagataa cggcctgaag atcatgtatc taagcaacta gcctgctctc
     1381 taataaaatg ttaggcctca acatctagtc gcaagctgag gggaaccact agtgtcatac
     1441 gaacctccaa gagacggtta cacaaacggg tacattgttg atgtcatgta tgacaatcgc
     1501 ccaagtaagt atccagctgt gttcagaacg tacgtccgaa ttccagacta tgtcgaacta
     1561 gataacgcgg aggagctggc cgacgcaatc attcgagagg ttgagttaaa ggaggcatgg
     1621 ctcacaaggg tcttggggaa ctcagccgat cctgacgctt ctgtgggcaa gtgggaccgg
     1681 cgcctagcgc agtgcggtgg tattgatgag atgtcaagtg gggtgcaggc aacatacttc
     1741 agtccaaacg gtcagctggt cggcttagtg gcatacattg atgccgaatg gatggatgaa
     1801 tagctctgac gttacgattc aggtgagaca accagatggg cattgcgtac aagctcagtt
     1861 gccaccgatg tggctacgag tctgatcttc tttacctagg ccaaggtatg gcgatgcttc
     1921 ctgagcaaat agtcggaacg tgcacatgct gcgacaatct aacgacgatt tctataaatg
     1981 aaagctacgg agtatgctca ctttgtgggg ctaaggatcg tgtgatattt acaagctatc
     2041 agcatgaggc aaggccaaga aatccctgct atctagagag aagtttcaaa tatcaatgcc
     2101 caagatgcca tgccttcagt atggagcctc cggatctacc ggagatgctt tgggactaat
     2161 gctccgcttt gcctcgggta tccgttcgac ctatgtttaa gaggaaacgc acgtgtctgc
     2221 cgccaaagct cttgcctatg cactttgtga caagttggaa agcaaaaaag ttgatgaagt
     2281 cgtacga
//
LOCUS       DAJTNN010000476         2234 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.guided.309, whole genome shotgun sequence.
ACCESSION   DAJTNN010000476 DAJTNN010000000
VERSION     DAJTNN010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.guided.309"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            264..923
                     /locus_tag="OO150_004460"
     CDS             264..923
                     /locus_tag="OO150_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5518346.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="OO150_004461"
     CDS             complement(1124..1501)
                     /locus_tag="OO150_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5518347.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..>2234)
                     /locus_tag="OO150_004462"
     CDS             complement(1568..>2234)
                     /locus_tag="OO150_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR5518348.1"
                     /translation="LGFRFAPRIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRA
                     HWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSV
                     ELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNT
                     VYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPL
                     QPA"
BASE COUNT          537 a          563 c          592 g          542 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caag
//
LOCUS       DAJTNN010000477         1683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703122
            SAMEA8900975-rid15821783.guided.328, whole genome shotgun sequence.
ACCESSION   DAJTNN010000477 DAJTNN010000000
VERSION     DAJTNN010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900975
            Sequence Read Archive: ERR6005296
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478210.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478210.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,465
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 183 of 324
            Pseudo Genes (incomplete)         :: 76 of 324
            Pseudo Genes (internal stop)      :: 101 of 324
            Pseudo Genes (multiple problems)  :: 34 of 324
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900975-rid15821783.guided.328"
                     /serovar="S. flexneri 6"
                     /isolate="703122"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-02"
     gene            complement(<1..212)
                     /locus_tag="OO150_004463"
     CDS             complement(<1..212)
                     /locus_tag="OO150_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HCR5518349.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQK"
     gene            544..1017
                     /locus_tag="OO150_004464"
     CDS             544..1017
                     /locus_tag="OO150_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5518350.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1112..1636
                     /gene="sat2"
                     /locus_tag="OO150_004465"
     CDS             1112..1636
                     /gene="sat2"
                     /locus_tag="OO150_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5518351.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
BASE COUNT          493 a          348 c          377 g          465 t
ORIGIN      
        1 attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa aaacagcctg
       61 acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat aaaacgctta
      121 atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg catatccgtg
      181 cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg caactgtctg
      241 cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg cctagagatg
      301 cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta tgagttcttt
      361 gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt gtaataacag
      421 agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta attaacggta
      481 agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct ctgaggaaga
      541 attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg gaatggccct
      601 gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac ctataaccaa
      661 tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa ccgaaagtat
      721 gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat ctttccatca
      781 attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt ttcaggtggt
      841 ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc tacaatagac
      901 atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag gccagttttt
      961 acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa gggttaacaa
     1021 gtggcagcaa cggattcgca aacctggcgc ggcttttgta ccaaaagccg cgccaggttt
     1081 gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc ctgagcaggt
     1141 ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg atgtgcacct
     1201 atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga aggattacat
     1261 ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg accaagagct
     1321 tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg aacacattgt
     1381 tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa
     1441 gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga acaatgtacc
     1501 tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc tgttcacgta
     1561 taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt tctcgggagc
     1621 acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg gcttaattca
     1681 gga
//
